LOCUS       DAOMPD010000001        31007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_659_length_31007_cov_3.397196, whole genome shotgun sequence.
ACCESSION   DAOMPD010000001 DAOMPD010000000
VERSION     DAOMPD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 31007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31007
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_31007_cov_3.397196"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PLY93_00005"
     CDS             <1..1389
                     /locus_tag="PLY93_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP67903.1"
                     /translation="TLWFGLLCVVIAFRVLLMGERFLHTIIPYSYWELLLKVEYLSMN
                     LGVIFFLLFLQFLFPFEMPRRALKILFVLTAILMLPILIFPTRVFSRLLVGYEVVILY
                     SVILCIVTLTTAIIRKRPGAFVIALSSFFLAFSVVNDVLYSQKLIFTFQMTGLGLFIF
                     IFSQSFILSMKFSKAFSTNEILSENLTRYNIAYERFVPREFLQYLAKDSILDIELGDQ
                     VQADMTIVFIDIRSFTALSEKMTPKENFNFLNNYLGRVSPIIRKYNGFIDKYLGDGFM
                     ALFPKRAEDAVLAVLEIQTAVQELNDEIRDKNLPQITIGAGIHTGRLMLGIVGEKERM
                     DGTVISDAVNLASRVEGLNKRFGSTLVISEDTLFQLDDPTRFSFRFLGRVYVQGKSAP
                     VSIFEFYDADPKDVRIVKTVTRTEFEKGVTAYYEEQLDDAHNIFQSILRANPTDRAVI
                     EYLRILTEKKIA"
     gene            1411..3207
                     /locus_tag="PLY93_00010"
     CDS             1411..3207
                     /locus_tag="PLY93_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRP67904.1"
                     /translation="MHSYGVSQQNNHLHFSVFVTDADNVELCFFDEKGNLITKHRLNA
                     DGNRWSLSLNAQKDIYRYAYCITRGKKSIFLADPFATNMNDLAAWRRKKTWPAPVSRV
                     EETPFDWQNDRAPNTPWNKTVIYEAHVKGLTQLLEEIPKTQRGTYLALAHKKIIAHLQ
                     SLGITALELLPVHGKSEDFFLHTKGLVNYWGYNTLAYFAPESEYALKDAVTEFKTMVR
                     ALHSAGIEVILDVVYNHTGEGGPDAPTVCFRGLAEKVYYRVNDDGAYVDYTACGNTLN
                     TDHPHTQEMVLASLRYWVETMHVDGFRFDLAPTIFREGETVDFTHALHQAIMSDPVLK
                     NVKFIAEPWDIGPNGYQRGRFPEPWREWNDIFRDAARRFWKGEGLARELAHAMTEKTE
                     NAIQFVTCHDGFTLHDLVSYEKKHNHANGEENRDGTDHNHSFNCGVEGTSANADVLAL
                     RQKQMRNFLFTLFFAQGTPMLRAGDEIAQTQQGNNNAYCQDNEISWIHWTTQDTALLQ
                     FLETLMRVRAEVKTTMARYGVSTEIKSDTAFEMFSGNYIFFFNAAPVSKIFSVKNFTL
                     KEILHTARNPHKERISGIILVSPQSAVLCELS"
     gene            3258..3674
                     /gene="arsC"
                     /locus_tag="PLY93_00015"
     CDS             3258..3674
                     /gene="arsC"
                     /locus_tag="PLY93_00015"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801920.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRP67905.1"
                     /translation="MVKLTGATAQGGSTTGGSPASAHAKVTIYYNHKCRKSREALQIL
                     KEKGIEPEIRNYMDEPPSKEELSEVLRKMGRRPRDIFRKSEPLYKDLGLKNKDLSDDE
                     LLGYLNEYPILIERPIVVKGQRAVLGRPPEDVRRII"
     gene            3708..4373
                     /locus_tag="PLY93_00020"
     CDS             3708..4373
                     /locus_tag="PLY93_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRP67906.1"
                     /translation="MAAQKIVVTSGPTREYFDPIRFLSNPSTGRMGHAIATAALKKNF
                     DVVFISGPVDADFLTVKGATNISVVSTQDMLQAVDTHVGAGDILVMAAAPADYRPLER
                     SPIKLKKSESPQIHLTPNPDILKHIAQKNKTLPVRATLVGFAAETHDTEKYALNKLKD
                     KELDMIFLNDVSKHGAGFGSTTNEFTVFFKDGSRLELANTTKAELGQKIIDCIIEKQN
                     LRL"
     gene            4370..5074
                     /locus_tag="PLY93_00025"
     CDS             4370..5074
                     /locus_tag="PLY93_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67907.1"
                     /translation="MIGILVATKNEAYFFLRYLTPVKKEGIYHYRGAIDGKKIALYLT
                     RPGIAAKEQLRRFLRLYSWQKIISTGACANLTSALKQRDVVRIGTAKAISKPTLFIPH
                     GEYTVVSAPVLVTDDSTKADIYRRTQADVLDMETYAIAAILSESEFNKIPWCAVRVVD
                     DTPGEEKYLYKERMLREMTVRTLSGRLKWRDIWAFGLWDYFRITTRRHRIAKAIFTVS
                     ICEGRRRPNLSKISRF"
     gene            5190..6482
                     /locus_tag="PLY93_00030"
     CDS             5190..6482
                     /locus_tag="PLY93_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRP67908.1"
                     /translation="MHTLGEKIRIYALTFFLAFYTATSSASISLQQIFYALALLAWLV
                     SFFLLRKSGAALPAFKWRTYYWLPVLWVVWRLFHVAISEFPIKELIQAREVWLILMLP
                     LTADIMAALRVMPQDKKPIQLFGLSPLYIVLIFLVISGALIGLFNAIEVVKSGGFENF
                     RAAGLNNNNALTYSGTSALTWMLALGFLLTVRKWQARPRFLLPLLWACFILMALAFIL
                     AKSRGGFIALILMGGFMSLVILRKKAILAWGAILVLSLGAWFGSSNLRNLFYAAIPKE
                     GSHTGTFEQRLDMWRAGVKMIQAKPIVGFGDAGYEAHYHEYKVEGAVNVAAAASHLHN
                     DFLNTWALYGAVGFTLFLLFFLWPLYDFVQIDTQVRSHPYWPLMAAALAGVWFMFFNG
                     LSQCHFTDEEVQTLLWFTIGVFYGTRDQILMSSLQKPT"
     gene            complement(6528..6860)
                     /locus_tag="PLY93_00035"
     CDS             complement(6528..6860)
                     /locus_tag="PLY93_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67909.1"
                     /translation="MIKKIVKRVSQLVVLLLLGVGIYLAVNFRHTAAFPKIISAFYSK
                     EFCSCYFVTEGTVDECHNFARQWVPIQSFSLDGENRSVTVKGLFVTTTARYTGDRYGC
                     VLEEWKWR"
     gene            complement(6853..8064)
                     /locus_tag="PLY93_00040"
     CDS             complement(6853..8064)
                     /locus_tag="PLY93_00040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRP67910.1"
                     /translation="MNPNTKVGVFFFIVLFVSLTACNPPPLIPAQVREALPVTFEVQA
                     KTNSYPGAEWSTTIPEKTGINREKLKVLEDYLFTTTGKEEERKGIRTDVFVLVRNGNL
                     VFERYGRNFHKNRKHLIWSTTKSFINTLLGMAVLDGKLKVEDTAAQYYTPLNVEGKNK
                     ILIDHLLRMSSGLYWSEGYEASPLKSSVIAMLYTRGRNDMAAFAAAQDLDYPPGMHWY
                     YSSGTTNILTAILKNVYTSDFENLIFHRLFKTIGMRFTTFERDGAGTPVGSSYIYTTG
                     QDLARLGYLYLHDGVWQGRRLIPEKWVRYTTTMAPAYYTTQLNEGDVDDNPGAQWYIN
                     QGIPERNQRPPWPDAPADTFAALGHWGQSLFVIPSLDIVAVRLADDRDGTFDKNKYLG
                     LLTQSVEVRHD"
     gene            complement(8064..8591)
                     /locus_tag="PLY93_00045"
     CDS             complement(8064..8591)
                     /locus_tag="PLY93_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRP67911.1"
                     /translation="MWIATLDHQIFEYLTLFANPPWLTALCERIGDMKSYAIPVIVFW
                     AVYLYRERMRALRFLFFLIAAFALSEFTAHLLKELFQRPRPAVEWLIYIDPKALGFPS
                     AHAMNTMVLAVLLADWFKKSFLWFLPLPLVVGASRVFANYHYPFDVIGGWFIGYVLGI
                     VVMRLKASANSEQWR"
     gene            8670..9593
                     /locus_tag="PLY93_00050"
     CDS             8670..9593
                     /locus_tag="PLY93_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67912.1"
                     /translation="MDNLKSEPLIALKGRFRAPNARYYGQFFILINQKNDCIDAAQVL
                     ITSNTETYNILKTTDNWKKFESLINRLKYEGKYSQSITSEFQERLKQLTLDKLFFSDL
                     VKAVHGNNENNLKFLVGGFYQRIIKDEEFSIDLLVDKASLNDESFENVGTTSPADSAL
                     ENGSYLPIKLDLDPISGKDVKQVRPGDKIFVRILPQSDQANAFIDSTGLRSESGFIKS
                     APFIITSVVYPGVGVELVGKLSDGVYGKIVEEQNVLIRVAETVKKITKPNGQANVSTP
                     QAEKSSILVMAIGVLGIVLLALILYFILAGN"
     gene            complement(9595..10866)
                     /locus_tag="PLY93_00055"
     CDS             complement(9595..10866)
                     /locus_tag="PLY93_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRP67913.1"
                     /translation="MSQLIIFKIVLGIIGALLFVFQIALWKRLLPWTRHLKWIKFYRY
                     LYFSILFFSQALIWIGVLYPGRGSSHAYPEWYIPIHKILLAINYTHLFLVLPFLFLWF
                     VGAIAKRFIKYRQKKNAVLEKNNTAENENLSRGDFLRKAGVTLYTGANLLPAVSSAAA
                     ISGMFLGSKEIWVNEKNITIQNLHNDLKGVRIAQISDSHIGNLIHEKYLNAAVDLIRA
                     AKLDYIFLTGDIIDNNNLFLPVAGKFIAALRKLVPWGHVVGVMGNHDYIDNGDKAAQS
                     FGDAGMNVLRNEIALFTRGKGKLQVVGLDYPPIRTSSRLTASKQYFDTVKKKLKEENP
                     TFVLNHHPSDFEYLKNEKIDFVFSGHTHGGQVRLSHNRESFLNSGSWFYKYYVDLYNE
                     NGVQLYVNRGLGHWFPLRIDCPPEITVFTLV"
     gene            complement(10870..13227)
                     /locus_tag="PLY93_00060"
     CDS             complement(10870..13227)
                     /locus_tag="PLY93_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP67914.1"
                     /translation="MANSVEKIHQRAFDIARDYKNSIITFEHLLLALTEDEIGLDILT
                     DLHCDIHALRKDLIGHIQTYDQDLDAINEITESAALSNFREMLLVRLILNNNREISIA
                     DIFDGIFRQHDSYAAYFLEKQGITRTKVLRYITHGINPDPLGDFEEDMTIPDGDVHDD
                     NMAPSKKQGDPDKILKQFTVDMTAEAAAGKYDKLIGREDELNRTMEILCRRNKNNALH
                     IGEPGVGKTAIMRGLAQRLVEDKVPDRIKGYQVFSLDVSSLVAGTRFRGDFEERVQMI
                     MAALAAKKKVILYIDEIHQIVGVCATRGDSMDAANMLKPVLTRGDMRCVGATTHEEYR
                     RYFEKDRALSRRFQKIDINEPSRDDAYAILLGLRKLYEDYHRVTYSDMILRRAVDLSA
                     EHINERYLPDKAIDVVDEVGASLHIYKPNQTEVTEDDLRGIVSRLAHVNLEKYSNAES
                     ASLKSIAENLKKRIFGQDAAIEKIARAVKRHRAGLGNKQKPIGSFLFTGPTGTGKTEL
                     TRAIAEELDVELIRFDMSEYMEAHSISRLLGSPPGYVGFDQGAQLTDAIRKHPRAVLL
                     LDEIEKAHIDIFNSLLQIMDYATITDATGKKADFRHVLLIMTSNAGSSELSTARIGFE
                     DKAKGAGHIKSALKTYFSPEFLNRLDDVVVFDRLGDTVLEKIVDKNLALLNEQIKEKE
                     IEVHLDAAARAHLAQKGYDPVMGARPMARLFQDTLRDLVTDAIIDERVQAGNDIFVTC
                     KNDTLIIKKIQDTKVVENNKKNLTTAKKITKAKGKQRSKKIHDAV"
     gene            complement(13229..13534)
                     /gene="clpS"
                     /locus_tag="PLY93_00065"
     CDS             complement(13229..13534)
                     /gene="clpS"
                     /locus_tag="PLY93_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRP67915.1"
                     /translation="MSTSIRHDDDTDVLTQIKKPKLYKVILLNDNYTTMEFVVYILQK
                     YFQKREDEAKRVMLDVHNNGSGIAGVYPREVAETKSKQVMAHAEQEGFPLACKTEPE"
     gene            13612..13947
                     /locus_tag="PLY93_00070"
     CDS             13612..13947
                     /locus_tag="PLY93_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP67916.1"
                     /translation="MDKVQIKEIAAGQAVAYLHGRLDVAVSSEIEEALLNAIDSKGIK
                     RLVLNMSDVEYMSSSGFRVMIAMLRKLKDLQGSLKICCVSPAVKRIFDVIELTTLFDM
                     YDTDEAALA"
     gene            13956..14783
                     /locus_tag="PLY93_00075"
     CDS             13956..14783
                     /locus_tag="PLY93_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRP67917.1"
                     /translation="MKQVFLLSKTSFCITFFLCFLPHCSSEAKNKALSHSNDHETNKN
                     LSISNEFLAGKFSPAKNQNFIEISSQYSSRKQYIDKRTFAAFEKMAIAAQKDGFDLTI
                     ISATRNFHTQKIIWDEKFNGKRKVGGENIAQTIPNEKKRALRILEFSSMPGTSRHHWG
                     TDIDLHEKKIKAPALYNSTFKSGRGKALHDWLTKNAPKFDFCQPYTGDPATRNGNSFQ
                     HGYREESWHWSYTPLSKLYLQQYTKRAKALKPSGYAGDNVAAHFYLDFVQNVDPTCR"
     gene            14786..16069
                     /locus_tag="PLY93_00080"
     CDS             14786..16069
                     /locus_tag="PLY93_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HRP67918.1"
                     /translation="MAIEKSLTLPCGSTLKNRLAKSAMSENLAVNGVPGDKLIRLYEK
                     WGQGNIGLVITGNIMVDGSELGEPNNMVLENDHSLDAYRKMTTAAKKHGSQIWAQINH
                     PGRQSPAAISKTTVAPSAVKLKIANIAFRTPHELTDAEILDIIQKFGRTAELCKEAGF
                     DGVQLHGAHGYLTSQFLSPLSNQRTDRWGGSIENRMRFPIEVMRAMRKAVGHKYPIGI
                     KINSADFQRGGFDEDDAVAVVKALEAEGVDLIEISGGTYEAAVMMRGAQEVPGSTRDT
                     MSLQAATGAQVRASTAAREAYFLPFAEKIKKSVKTPIMLTGGFRTSLAMNQAIEGGIV
                     DVVGIARPLAMEPLVCIRLLRDANARIEMQPVKTGIAILDRMGVLEIGYYSLQLKRIG
                     EGKDPDKNLSPLLALPLLGLNYSSGILSRLLNRAS"
     gene            16122..16952
                     /gene="dapF"
                     /locus_tag="PLY93_00085"
     CDS             16122..16952
                     /gene="dapF"
                     /locus_tag="PLY93_00085"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRP67919.1"
                     /translation="MKFTKMHGLGNDFIVVDERTQTINADFVSLAKKLCDRHQGIGAD
                     GLIRVLPSTKGDFKMEIINSDGSIPEMCGNGIRCFAKFVFDNEVNPKKEYKIETGAGV
                     LTPSLEIKNGKVIGVTVDMGEPILTPAKIPMLGYDSHLNQKITAGGVSYTFTAVSMGN
                     PHIVIFEPDLSHVELSHVGPFLERHAAFPARTNVHFVSIVSPTEIRVRHWERGAGETK
                     ACGTGACACVVAGSLTDRTERNVLVHVPGGTLKINWDETTNHVFMQGAAESVYQGELF
                     "
     gene            16984..18171
                     /locus_tag="PLY93_00090"
     CDS             16984..18171
                     /locus_tag="PLY93_00090"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRP67920.1"
                     /translation="MSPLTKAIRAKRISNTPPYLFAEIDKKKAAAIQKGINLISFGIG
                     DPDLPTPKHIVDAMHAAIDDAKTHDYPPYEGTKEFREAVAVWMYKRFGVSIDPVKEAM
                     ALIGSKEAIAHIHTALVDKNDVCLIPSPSYPVYRVATIFSEGIPYNMPLKEANGFLPD
                     LKKIPADVLAKAKLIWVNYPNNPTGAVCDLSFYEELVAFAKKNNIIICSDNAYSEMTY
                     DGFIAPSILQIPGAKDVAIEFLSLSKAYNMTGWRLGMAVGNEELIAALGIVKTNTDSG
                     QFKAIQKAGAMALTAPQESIREMNAIYQKRRDAMVGGLNSLGWNIQPIKGTFYIWAKI
                     PPRFNSSADFCGHVLDETGIVLVPGNGYGDEGEGYFRAAITVSVEKIEEGIRRLKEKK
                     ITF"
     gene            18233..18547
                     /locus_tag="PLY93_00095"
     CDS             18233..18547
                     /locus_tag="PLY93_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67921.1"
                     /translation="MRTILLFLVVACVVGVVHANPIASNCTFKGHKLYGKVKIVESFP
                     DIKIKIVESFPDLKVKQVSSFPDRCGKWQFVESFPDVKIKFVESFPDIKIKYVESFPG
                     IN"
     gene            18550..19482
                     /locus_tag="PLY93_00100"
     CDS             18550..19482
                     /locus_tag="PLY93_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP67922.1"
                     /translation="MKKKIFVIILILLVALAAGVVSQIRFDIPAAEIEAKYKLPESKF
                     IDVNGLRTHYTDEGVKKGSHETVVLLHGTAASLNTWQAWMPELKKHFRVVRVDLPAFG
                     LTGPSPKRDYSIKAYVKFLEDFLSTLKIKQIYLVGNSLGGEIAWHYAAEHPDDVYKLI
                     LIDSAGLQRKGKMPLAFWLARKKYLQGLVKYFSPRFLIKKSLKDVYFDKSKVTDALVD
                     RYFLMTLREGNRQAFIDRANQIESATDDDLRQVRSPTLILWGRHDAWIPVEQAEIFRK
                     KIMIPQLKIYENAGHVPQEEIPDETLVDVIKFLD"
     gene            19488..20279
                     /locus_tag="PLY93_00105"
     CDS             19488..20279
                     /locus_tag="PLY93_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRP67923.1"
                     /translation="MQTLYEKKQLRATLDKNDVCRVYLNNPAQLNALNTTMAEGAVEL
                     ATIIKASPARVVVFSGEGRAFSAGGDLDYLLHLTTLPKEESAAAMLKFYQSYLTLFTL
                     PLATIAHINGPCVGAGFCLALACDLRYVVSGTKVGMNFVRIGLNPGMAAEAWAVRSDE
                     VLLREMLLTGKIYSSSEPRLRTLFNLVASPEEIRTAVEDTATQITSASPQSIRLSLPL
                     LRNAHLTREAVMHAEAEGQGISMSTGEIKEAVAAQRAGKIFRFNL"
     gene            complement(20274..20996)
                     /locus_tag="PLY93_00110"
     CDS             complement(20274..20996)
                     /locus_tag="PLY93_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP67924.1"
                     /translation="MCTSNDTRDKRLRASLFFQSKEGNVLVDSGPDLREQMLRKKLTT
                     VNAVLYTHFHFDHLDGLPDLRPFTFDSKDELKCYANPQTHEVILTRYPYIRNSAIYTN
                     VPRIGLHLYPGDEEDGYRAVEIAGMEFQPIRLVHIPQSGVLSTGFVVNKKFGYLTDFK
                     EIHHNDEKYLYNLDILYLGSPIDHPHVSHIQQQEALELFKKFNAKRGVIGHLSHQYMH
                     EELLARWQGQAEPAFDGQEFHL"
     gene            complement(21069..22091)
                     /locus_tag="PLY93_00115"
     CDS             complement(21069..22091)
                     /locus_tag="PLY93_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRP67925.1"
                     /translation="MRKLLSCCVIMAAVFTTTVCGGNPQDEVLATFDGGKITQKDVEK
                     DNAQEFFEIRQKEFEIRQQAAYENARDKIFAAEAKKQNLTVEKYVETELAKRRGAPVT
                     DASVKQFYEMNKHRINMPFETIKDRIRQQLLNNQEKSARDGLTAELFKQYNFKFTLTE
                     PEAPKLNLVNDGKPFWGKSDAKVVITEFSDFECPYCRQMQPDVQRLKAEYSNKVKWVF
                     RNFPLDFHAQAMPTHIAAECAGKQNKYFEFQKKAFEIPYQGRSLDLSTTQLDKIAAAI
                     GVNMNAYKQCIADKDGVVRASIEDDMRYGQKIGVRGTPTIFINGVLYQQERSYDAIKK
                     YIDKLL"
     gene            22434..24092
                     /locus_tag="PLY93_00120"
     CDS             22434..24092
                     /locus_tag="PLY93_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67926.1"
                     /translation="MTTTHQAGFRYRWIGIGDLNAFFGLMLDNVTNLVFLAQILILVF
                     GFPANLVYGKMFPGTAFGVLVGDLIYTAMAFRLAKRTQNPNVTAMPLGLDSPSTIGIA
                     FAVIGPAFIAAKNSGLDPQAAGLLAWKVGMATLVIIGVVKFILSFFGDFVEKLIPTAG
                     LLGSLAGVGIALLGLLPLEHVFGTPVAGLVALGIIIYALVAKVRLPFSIPSVFLSVIA
                     GTVIYYILSALHFVHPPEISLKFIIAFPMPTLEFLGGFKEAFAYLPVAIPFALLTVIG
                     GINVTASARLAGDSYRTSSILLTEAVATLAAGVTGGVAQSTPYIGHPAYKAMGGRAGY
                     TLATGLFIGLGGVLGFISFLAQLLPQSALAPILIFVGIDIVEQSFHATPKRHAMAVAM
                     AILPAMPYLAKIKMQGLVFQIQTLYASLAQASAETAAKLKPTLDFFGSAFQESWFTIV
                     ALGNGFIVTAMIWGAFTAKLIDREFYKAAAYLFVGAFLSLFGLMHSIKIDGDMYLPWS
                     ITVNASGPTPYSIAAAYITLAIVIIMLSYSKGAKEAKAAKLDLD"
     gene            complement(24084..24887)
                     /locus_tag="PLY93_00125"
     CDS             complement(24084..24887)
                     /locus_tag="PLY93_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67927.1"
                     /translation="MPTNRLVERTVSRLNSQKAVRLQINALTKEGRKAIQSVLLEFFE
                     KESLDKKHVLPIHYAIVELVFNALKANIKFVAFREEIRKELNRFEMTEIEDFLQLILN
                     ERALREFAATRILPDVLRGEVQNVFNLEEKYRAGMSKKMSDDQIQLIAKFRELIRSIN
                     ADVLLEIHATPDEIRIQVLNNVPMLQRDLERIQNSRIRHAALHKQGKGGEFFSYDNMD
                     TTESAGFGIAMVDQGFYQLGLDPFEHMKIEAKNKETEATLIYPRSSLSI"
     gene            complement(24978..26372)
                     /gene="flgE"
                     /locus_tag="PLY93_00130"
     CDS             complement(24978..26372)
                     /gene="flgE"
                     /locus_tag="PLY93_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRP67928.1"
                     /translation="MMRTLYAGVSGLKNHQVRMDVIGNNISNVNTNGFKSERVTFQDM
                     ISQLMSGASEPRQNVGGVNPKQVGLGAMTASIDKLMHQGALQTTSKNTDLAISGEGFF
                     VLADGSKKFYTRNGNFDVDKEGWLVNPANGMRLQGWNATKSADGKMGVNTAADTENLK
                     VPLYQKEPAKETSFVRFRSNLNSSVEAIPAGASDADRVKMIMDADPSKRRGHITSINV
                     FDDQGVEHTVKMYLWKSDTNKWTASVAMQGAEQLSIDVAGSNGQNTQVAGNTRMTLGF
                     SPDGRLTSVSDGADVRNQDNLTANISFRLAGNPAVKNIELRLGEAGQVDGITQFSSAF
                     TTKAVEQDGYTMGYMEAFEINDTGVITGVYSNGARNTLGQVALANFANPEGLTKSGQN
                     NFVESINSGAALVGTSGIQGLGGINAGFLEMSNVDLADQFTDMIVTQRGFQANSRSIT
                     TADQMLQELLGLKR"
     gene            complement(26369..27073)
                     /locus_tag="PLY93_00135"
     CDS             complement(26369..27073)
                     /locus_tag="PLY93_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRP67929.1"
                     /translation="MPDINNVQTQDADVTRLREFQRSRQRQPVSHNGKTPLVRKSGYD
                     ALDKNSFLKLLVTQLSKQDPTNPMNDREFISQMAQFSSLEQMNNVASSMNKLRNAQAN
                     TLIGKMVEGKDFVTEKHLQGIVTAAIYEPNGDVLLKVGGRFTKFDDITSVTEVSPSSA
                     NIPNGQRPSPLSNGAESGMSRQPVPNVSRETSITPIKTSTSEVAKAYEENQTQKVVSP
                     STVEATRKVSKGEQEK"
     gene            complement(27066..28484)
                     /locus_tag="PLY93_00140"
     CDS             complement(27066..28484)
                     /locus_tag="PLY93_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67930.1"
                     /translation="MLSLNILNSTPTHVSALPKNNAKTQAANGDKSVMDFSSYIAMVA
                     DAAHNAATQPANTTTTTAPAEKGYESSPVDGTAAQRANEDIVSKNEKPQHAQKTGINE
                     NSPATTKASTNTRPENQLSESLGLLGKNAATQVSKAAQKHLAIKKEDPTLAVNLSHTI
                     PLHSHKGTIKNAAISTALLQLQKTPDTLLLNVDGLRRLSEKLGVSFLQKVEEKLNAKK
                     DSQKSHTPQSKTTSKEWVSTEALNNRVQKAGDTPPQNNAKNTTFLSKAEMHGKKAHIV
                     SRETISTNVIEKQPTVNTVELKPITHEKNNQTPVDIRLSDSVSSVRTQTIHRTAIENT
                     TTPAALSRQLTDVMARAHVLIADSQNAQFTVKLFPREIGRMEIDLKMVEGEIRGKIVV
                     ENEEVKSELQNFLNQNNNKEGESVDLNRVNIEVRNGNQQAANPEKSRTEEALLQDLVV
                     RAATSAYEASAGEVVKNGGLYA"
     gene            complement(28668..29633)
                     /locus_tag="PLY93_00145"
     CDS             complement(28668..29633)
                     /locus_tag="PLY93_00145"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRP67931.1"
                     /translation="MRIESRQIVVNDTESGLRLDLFLTRRFTKFSRNKWQTRIDEKTI
                     RINGIVPKQARKLNVGDTIEFSYTMREEPEVNTRIETIYEDKDYFVVDKPAGLPVHPS
                     GIYKTQTITTLLVERRLLTTPYLLHRLDRETSGVLALAKNRTAAVAFHRILRAGALKK
                     EYVVAVEGSVDTPIDAEGFIFRLPTSRLPRQRYFSTNPPAVAMEIQRCHTLFTPLLTN
                     NNLTLLRAQLFTGRMHQIRATLSSLGYPVVGDKLYGINHDFYFKFADDTMTHDDWDSL
                     RIARSALHCEKLTLAHPVSGKPWPLLAEIPADIQSIFSNTPKESK"
     gene            29771..>31007
                     /locus_tag="PLY93_00150"
     CDS             29771..>31007
                     /locus_tag="PLY93_00150"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRP67932.1"
                     /translation="MVELNRYSKTLTQDPSQPASQAMFYGIGFKDEDFSKAQVGIVSM
                     GYDGNTCNMHLNGLAAEAKKSVWENGMVGLVFHTIGVSDGMANGTDGMRFSLVSRDVI
                     ADSIETVCGAQYYDALIAIPGCDKNMPGSLIAMGRLNRPAIMLYGGTIAPGKYKGKDL
                     NIVSSFEALGEKIAGKISDEEFHEIVCHSCPGAGACGGMYTANTMAAAIECLGMSLPY
                     SSSNPALSPEKIGECKEVGAAVKNILEKNILPRDIMTKKAFENAITLIMVIGGSTNAV
                     IHIIAMAKSVGISVTQDDFQRISDKTPLLADFKPSGKYMMQDLHEKGGIPAVMKYLLN
                     RGMLHGDCLTVTGKTIAENLKDVAEIDFTKQDIIYPIEKPLKATGHLQILYGNLAKGG
                     SVAKITGHEGERFTGPARVF"
BASE COUNT         8184 a         7150 c         7059 g         8614 t
ORIGIN      
        1 actttatggt ttgggcttct ttgcgtggtt atcgcgtttc gtgttttgct catgggggaa
       61 agatttttgc acacaattat tccttatagt tattgggaac tcttacttaa ggttgaatac
      121 ctatcaatga acttgggggt aattttcttt ttactgtttt tacagttttt gtttcccttc
      181 gaaatgccac ggcgagcttt aaaaattctt tttgtgctca cagcaatact aatgctcccg
      241 attcttattt ttccgacacg ggtattttca cgcctccttg tcggttacga agtggttatt
      301 ttatacagcg taattttgtg catcgttacg ttgaccaccg caatcatacg caaacgtccg
      361 ggcgcattcg taattgcctt atcgagtttc ttcttggcgt tctctgtggt gaatgatgtg
      421 ctctactcac aaaaattaat tttcacattc cagatgacag gtttggggct ttttattttt
      481 attttttcac aatcctttat tttgtcgatg aaattctcaa aagcattttc aacaaacgag
      541 atcctttctg aaaatttaac gcgctacaac attgcctacg agcggtttgt gccgcgtgag
      601 tttttgcagt atttagccaa agatagcatt ttagatattg aactgggcga ccaagtacaa
      661 gccgacatga cgattgtttt tatcgacatc cgttctttca cagcgctttc tgaaaaaatg
      721 acaccgaaag aaaactttaa ttttttgaat aattatttag gccgcgtcag tcccatcatc
      781 cgtaaataca acggctttat cgataaatac ttaggcgacg gttttatggc tctcttccct
      841 aaaagggcag aagacgccgt gctcgcggtg ttggaaatcc aaaccgctgt tcaagaactc
      901 aacgatgaaa tccgcgataa aaatttaccg caaataacaa tcggtgctgg cattcacacc
      961 ggccgcctca tgctgggcat cgtcggtgaa aaagaacgca tggacggcac agtcatttcc
     1021 gacgcggtga atttagcatc gcgtgtcgaa gggctcaata agcgttttgg ttcgacactc
     1081 gtcataagcg aagatacgct ttttcaactc gacgatccca cacggtttag ttttcgtttt
     1141 ttgggacgcg tttatgttca ggggaaaagt gcaccggttt cgatttttga attttacgat
     1201 gcagatccca aggatgtgcg tatcgttaaa acggtgacgc gcacagagtt tgaaaaaggg
     1261 gtcaccgcgt attacgaaga acaactcgac gatgcacaca atatcttcca aagtatttta
     1321 cgcgccaacc ccacagacag agccgttatt gaatacctgc gtattttaac cgaaaaaaaa
     1381 attgcttaaa gagcgtaggt tgagtcgata gtgcatagtt acggcgtttc gcagcaaaat
     1441 aatcatttac atttttctgt ttttgtcacc gacgccgaca acgtcgaact ttgttttttt
     1501 gatgaaaaag gaaatcttat aacaaagcat cgcttaaacg cggacggcaa ccggtggtcg
     1561 ctttcgttga acgctcagaa agatatatac cgttacgcgt attgtatcac acgcggaaaa
     1621 aaatccatct ttttggccga tcctttcgcc acaaatatga acgaccttgc ggcatggcgt
     1681 agaaaaaaaa cttggccagc gccggtatcg cgcgtggaag agacaccctt cgattggcag
     1741 aacgaccgtg cccccaacac accgtggaat aaaacagtta tttacgaagc gcacgttaaa
     1801 gggctcactc agttattaga ggaaatacct aagacgcaac gcggtacgta cttagccctt
     1861 gcacacaaaa aaataatcgc gcatcttcag tcgttgggga tcacagcact tgagctttta
     1921 cccgtacacg gtaaaagcga agattttttt ctacacacaa aagggctcgt caactattgg
     1981 ggctataaca cgctcgcgta ctttgcgcca gagagcgaat atgcacttaa agacgctgtg
     2041 actgagttta agacgatggt gcgtgcgctc cacagcgccg gtatcgaagt cattcttgac
     2101 gtggtctata accacaccgg cgaaggtggg ccggatgctc caacagtgtg ctttcgcggt
     2161 cttgcggaaa aagtatatta ccgtgttaac gacgacggcg cgtacgtcga ctacaccgca
     2221 tgcggcaaca ccctcaacac cgaccacccg cacactcaag aaatggtttt agcgagtttg
     2281 cgctattggg ttgagacaat gcacgtcgat ggttttcgat ttgatttagc gccgactatt
     2341 tttcgcgaag gagagacagt cgacttcacg cacgcgctac accaagccat catgagcgac
     2401 cccgtgctca aaaatgtaaa atttatcgcc gagccatggg atattggccc caatggttat
     2461 cagcgcgggc gcttccccga gccgtggcgc gaatggaacg atattttccg cgacgccgca
     2521 cgccgctttt ggaaaggcga aggccttgct cgcgagctcg cacacgcgat gaccgaaaaa
     2581 acggaaaacg ccatccagtt tgtcacatgc cacgacggct tcactctcca cgatttagtg
     2641 agctacgaaa aaaaacacaa ccatgccaac ggcgaagaaa accgcgacgg tacagaccac
     2701 aaccacagct ttaattgcgg cgtggaaggt acaagcgcga atgcagatgt cctcgcgcta
     2761 cggcaaaaac aaatgcgtaa ttttttgttc actctttttt tcgcacaagg cacgccgatg
     2821 ctgcgcgcgg gcgatgagat tgcacaaacg caacaaggaa acaacaacgc gtactgccaa
     2881 gacaacgaaa tttcgtggat ccactggaca acgcaagata cagcgttact ccaattttta
     2941 gagacgctca tgcgcgtccg cgccgaagta aaaacaacaa tggcgcgtta tggagtttct
     3001 acagaaataa aatcagatac tgcttttgaa atgttttccg gcaattatat tttctttttt
     3061 aatgccgccc ccgtcagtaa aatattttct gtgaaaaatt ttaccctcaa agaaatatta
     3121 cacaccgcac gcaatcctca taaagagaga ataagcggca ttattcttgt gtctccccaa
     3181 agtgctgtac tttgcgagtt gagttgagag gactgtattc accttgacgg cgtgtctcga
     3241 cgagagtttc agtcattatg gtgaaactta ctggagctac ggcgcaaggc gggagcacca
     3301 caggcggttc acccgccagt gctcacgcaa aggtaactat ctactacaac cataaatgcc
     3361 gcaaaagccg cgaagctttg caaatcttga aagaaaaagg cattgaaccc gaaattcgca
     3421 attacatgga tgagccccct tcaaaagaag aactttctga agtgttgcgt aaaatgggac
     3481 gtcgaccacg cgatattttt cgtaaatcag aaccgcttta caaagattta ggcttaaaga
     3541 ataaagactt gagcgatgac gaattactcg gttatttaaa cgaatacccc attttaatcg
     3601 agcgacccat cgtcgtaaaa gggcagcgtg ccgttttagg ccgtccccct gaagatgttc
     3661 gtcgcatcat ttgatcgact ttctttgatc gcaatacgag gataatcatg gcggcacaaa
     3721 agattgtcgt cacctctggc cccacccgtg aatatttcga tcccattcga tttttgtcga
     3781 acccatcgac agggcgcatg gggcatgcta ttgccacagc agcgctaaaa aaaaattttg
     3841 acgttgtttt tatttcaggt cctgtcgatg cagacttttt aacagtgaag ggtgcgacga
     3901 atatatcagt tgtcagcacc caagatatgc ttcaagctgt ggatacacac gttggcgctg
     3961 gagacatact tgtgatggca gcggcaccgg cagattatcg accactggaa cgctcgccga
     4021 ttaaactaaa aaaatctgaa agtccacaaa tccacctaac acccaacccc gacatcttaa
     4081 aacatatcgc acagaaaaat aaaactttgc ctgtacgcgc aactcttgtg ggctttgccg
     4141 ctgaaactca cgatacagaa aaatatgcgt tgaacaaact caaagataaa gaactcgata
     4201 tgattttttt aaacgacgta tcaaaacacg gcgctggttt tggctcaaca acgaatgagt
     4261 tcactgtgtt ctttaaagac ggtagtcgtc ttgagctcgc taatacgaca aaagctgagt
     4321 tggggcaaaa aattatcgac tgcatcattg aaaaacaaaa cctccgcttg tgattggtat
     4381 tcttgtcgca accaagaacg aagcgtactt ttttttgcgc tatttaaccc ctgtaaaaaa
     4441 agaaggtatt tatcattatc gtggcgccat cgatggtaaa aaaattgcac tctatctgac
     4501 gcgcccaggt attgcagcca aggaacaatt acgccgattt ttgcggcttt attcgtggca
     4561 aaaaattatt tcgacaggag catgcgccaa tttaaccagt gcattaaaac agagagacgt
     4621 ggtgcgtatc ggtactgcaa aagccatcag caaacctacg ctctttatcc ctcatggcga
     4681 atacaccgtc gtttcagcgc cagtactcgt gacagacgac tccacaaagg cagatattta
     4741 tcgacgcacc caagcggatg tgctcgacat ggaaacttac gccattgcgg caattctttc
     4801 cgaatctgag tttaataaga taccatggtg tgcagtgcgg gttgtggacg atacccctgg
     4861 tgaagaaaaa tatttatata aagaaagaat gttgcgcgag atgacagtgc ggactctatc
     4921 gggtagattg aaatggcgtg acatttgggc gtttgggttg tgggattatt ttcggataac
     4981 aacacgacgg catcgtattg ccaaggcgat atttacagtt tcgatctgcg agggacgaag
     5041 acgcccgaat ttgtccaaaa tttcacgctt ttgagcgaag cgcgtacaaa aatttgcaaa
     5101 aaaattgcct ctttctacac actgcatgag ttggcgcaca ggctcttagg cattgacacc
     5161 ttatagaaca tcaccgcacc gtatgttcta tgcacactct cggtgaaaaa atacgcatct
     5221 acgcgctaac tttttttctg gctttttaca ccgccacttc ttcggcttca atttctctac
     5281 aacaaatttt ttacgcgctc gctctattgg cgtggctcgt ttcgtttttt cttttacgca
     5341 aaagcggcgc agcgctacca gcgttcaaat ggcgcacata ctattggttg ccagtgttgt
     5401 gggtggtatg gcgtttattt cacgtcgcta tttcagaatt tcctatcaaa gaactcatcc
     5461 aagcgcggga agtgtggctc attttgatgc tgccactcac tgccgatatt atggccgcgc
     5521 ttcgggtgat gccgcaagat aaaaaaccta tccaactttt tggattatcc cctctttata
     5581 ttgttttaat ttttttggtt atctccggtg cactcatcgg cttgttcaac gcgattgaag
     5641 tggtgaaaag cggcgggttt gaaaacttcc gcgccgctgg tctcaacaac aacaacgcgc
     5701 tcacttattc aggcacttcg gcgctgacgt ggatgcttgc gttgggtttt cttttaaccg
     5761 tgcgtaaatg gcaggcgcgg cctcggttcc ttttgccgct tttgtgggcg tgctttattc
     5821 tcatggcttt ggcatttatt cttgcgaaat cgcgcggcgg ttttatcgct cttatcctca
     5881 tggggggctt tatgagcctt gtgattttac gcaaaaaagc aatccttgcg tggggtgcaa
     5941 ttttagtact ctctcttggc gcatggtttg gttcttcaaa cttacgcaat ttattctatg
     6001 cagcgatacc taaagaaggc tcgcatacag gtacattcga gcaacgcctc gatatgtggc
     6061 gcgccggcgt caaaatgatc caagccaaac ccatcgtagg atttggtgac gcagggtatg
     6121 aggcacacta ccatgaatac aaagtcgaag gcgctgtcaa tgtggctgcc gctgcatcgc
     6181 acctccacaa cgatttcttg aacacatggg cgctttatgg cgcagtggga ttcacactgt
     6241 tcctactttt ttttctgtgg ccgctttacg attttgtgca gattgacact caggtgcgtt
     6301 cgcacccata ttggccactc atggcagcgg cgctcgcagg agtctggttt atgtttttta
     6361 atggactctc gcagtgccac ttcaccgacg aagaagtgca aacactgtta tggttcacca
     6421 tcggtgtgtt ttacggcacc cgcgatcaaa ttcttatgtc atcgcttcaa aaacccacgt
     6481 agaaattaat gccgtttatc ctaatcgcag tgcgaattaa ttttttatta cctccatttc
     6541 cattcttcca acacacaccc ataccgatcg ccggtgtagc gcgcggtggt agtcacaaat
     6601 aaacccttca ctgtcacgct gcggttttca ccgtcgagtg aaaacgattg aataggtacc
     6661 cactgccgcg caaagttgtg gcattcatcc accgtacctt ctgtaacaaa atagcatgag
     6721 cagaattctt tgctgtaaaa tgccgaaata attttgggaa atgccgctgt gtggcgaaaa
     6781 ttgacggcaa gataaatacc aactcctaaa agaaggagta ccaccagttg cgatacccgc
     6841 ttcacaattt ttttaatcat ggcgcacctc aacgctttgg gttaaaagcc ccaaatattt
     6901 atttttatca aacgtaccgt cgcggtcgtc cgccaagcgc accgccacta tgtcgagaga
     6961 cggtatgaca aagagcgatt gcccccagtg ccccagcgca gcgaatgtgt ctgcgggagc
     7021 atccggccaa ggcggccttt ggttacgctc gggaatgcct tgattgatat accactgcgc
     7081 acccgggttg tcatccacat cgccttcgtt aagctgagtg gtataatacg ccggcgccat
     7141 ggtggttgtg tagcgtaccc acttttcagg gatgaggcgc cttccttgcc aaacgccatc
     7201 gtgcagataa agatacccca aacgcgccaa atcttggccg gtggtataaa tgtacgacga
     7261 gcccaccggt gtgcccgcgc cgtcacgctc gaatgtggtg aagcgcatac cgatggtttt
     7321 gaaaagacgg tgaaaaataa ggttttcaaa atcgctcgta taaacatttt tgagaatcgc
     7381 cgtcaaaata tttgttgttc cgcttgaata gtaccaatgc atgccgggtg ggtaatccaa
     7441 atcctgcgcg gcggcaaatg cagccatgtc gttacgcccg cgcgtgtaga gcattgcgat
     7501 gaccgacgac ttcaaaggtg atgcttcata gccttccgac caataaagcc ccgagctcat
     7561 gcgcaacaaa tgatcgataa ggattttatt tttcccttca acattgagcg gcgtgtagta
     7621 ttgcgcagcg gtatcttcga ctttgagttt accgtccaaa acggccatgc ccaaaagcgt
     7681 attgataaaa ctttttgtcg ttgaccaaat aagatgctta cggtttttat ggaagttccg
     7741 accataccgc tcaaacacaa gattaccgtt acggaccaac acaaacacat cggtgcgtat
     7801 tcctttgcgc tcctcttctt taccggtggt ggtaaataga taatcctcta aaacttttaa
     7861 cttttccctg ttaatccccg ttttctcggg gattgtcgtc gaccattcag cgccgggata
     7921 actattcgtt ttggcttgta cttcaaacgt gaccggcaag gcttcgcgta cttgagcggg
     7981 aataagcgga ggcggattgc aagcggttaa agaaacaaat agaactatga aaaaaaagac
     8041 accgacttta gtgtttggat tcatcaacgc cactgttctg aatttgcact ggctttcaat
     8101 cgcattacta cgatacccaa aacataccca atgaaccatc cccctatgac gtcaaaagga
     8161 tagtggtagt tcgcaaacac acgcgaagca ccgacaacaa gtggtaatgg taaaaaccag
     8221 agaaatgatt tcttaaacca atcggctaat agaaccgcga gaaccatagt attcatggca
     8281 tgagcggatg gaaaaccgag cgcttttgga tcaatgtaaa taagccactc caccgcaggg
     8341 cgcggtcttt gaaaaagttc tttgagcaga tgtgctgtaa attcactcaa cgcaaaggct
     8401 gctataagga agaaaagaaa acgcagtgcg cgcattcgtt cgcgataaag ataaactgcc
     8461 caaaaaacaa tcactggaat cgcatacgac ttcatgtcac cgatacgttc gcaaagcgcc
     8521 gtaagccacg gtgggttggc aaaaagtgtg agatactcaa aaatttgatg gtcgagcgtg
     8581 gcaatccaca caggatatgc gcacaagtaa gcaccgccat gtcaaccgct aaacgcttga
     8641 cgcaatcgcc aaggattata cacggcgcaa tggataatct caagagcgaa cctttgattg
     8701 cgctcaaggg tcgctttcgc gctcctaacg cgcgctacta tggtcagttt ttcatactta
     8761 tcaatcaaaa aaacgactgc atcgacgctg cccaagttct catcacctca aatactgaaa
     8821 cctacaatat acttaaaacc accgacaact ggaaaaaatt tgagtcgctc atcaatagac
     8881 tcaaatacga aggtaaatat tcacaatcga tcacgagtga atttcaagaa cgactcaaac
     8941 aactgacatt ggataagcta tttttcagcg atttagtaaa agcagtgcac ggcaataacg
     9001 aaaataatct taaatttctt gtcggcggat tttaccaacg cattatcaaa gacgaagaat
     9061 tctcgattga cttactcgtc gataaggcat ctttaaatga cgagtctttc gaaaatgttg
     9121 gaaccacctc ccccgcggac tcggctcttg aaaacggttc gtacttgcct atcaaactcg
     9181 acctcgatcc aatttcagga aaagacgtga agcaagtgcg ccctggcgat aaaatctttg
     9241 tgcgaattct gccccaaagt gaccaagcga atgcattcat cgattctacg gggttgcgct
     9301 ccgaaagtgg atttattaaa tcggcgcctt ttattataac gagtgtggta tacccaggcg
     9361 tgggcgttga attggtggga aaactctccg acggcgttta tggaaaaatt gtcgaagaac
     9421 aaaacgtact catccgtgtt gccgaaactg taaagaaaat aacaaagccc aatgggcagg
     9481 cgaacgtatc gacgcctcaa gcagagaaaa gctccatttt agtgatggca atcggcgttt
     9541 taggtattgt tcttttggcg cttattcttt attttatttt agcaggtaat taatttacac
     9601 taaagtgaac accgttatct cgggtggaca atcaatacgc aacggaaacc aatgtcccaa
     9661 ccctcggttg acgtaaagct gcacgccatt ttcgttgtat aaatcgacgt aatacttgta
     9721 aaaccaacta ccactattca aaaacgattc gcggttgtgc gaaagtcgca cttgcccccc
     9781 atgcgtgtga ccagaaaaga caaaatcaat tttttcgttt tttaaatatt caaaatcgga
     9841 cggatggtga ttcaacacaa aagtgggatt ttcttctttc aacttttttt tcaccgtatc
     9901 gaaatattgt tttgaggccg tcaaacgcga actggtgcgg atgggtgggt aatccaaccc
     9961 cacaacttga agtttacctt tcccgcgcgt gaagagcgct atctcgttgc gcaaaacatt
    10021 catccccgcg tcgccaaaac tctgcgcagc tttatcgccg ttgtcgatgt aatcatgatt
    10081 gcccatcacg cccacgacat gcccccacgg gacaagtttg cgtaacgcgg cgataaactt
    10141 acctgcgacg ggcaaaaata aattattgtt gtcaatgatg tcgccggtca aaaaaatata
    10201 atcgagtttc gccgctcgga ttaaatcgac cgctgcattc aaatattttt cgtgaattaa
    10261 gttgccgatg tggctatcgg aaatctgcgc aatacgcacc cctttgaggt cgttatgcaa
    10321 attttggatg gtgatatttt tttcattcac ccaaatttct ttcgaaccta aaaacatccc
    10381 actaatggcc gccgcactcg acacagccgg caaaagattt gcgccagtat agagagtcac
    10441 tcccgcttta cgcaagaaat ctcccctgga gagattttca ttttcggctg tattattttt
    10501 ttcgagaacg gcattttttt tctggcggta ttttataaac cgcttggcaa tcgcacccac
    10561 gaaccaaaga aaaagaaacg gcagcaccaa aaaaagatgg gtatagttaa tcgctagtaa
    10621 aattttgtgt atcggtatat accactctgg gtatgcatga ctgctaccgc gtccggggta
    10681 caaaacgcca atccaaataa gcgcttgcga gaaaaaaaga atactaaaat aaagatagcg
    10741 ataaaattta atccatttga ggtggcgcgt ccacggcaac agtcgtttcc aaagtgcgat
    10801 ttgaaaaaca aacaacaacg cgccgattat acccagaacg attttgaata taatcagttg
    10861 gctcatacgc taaacggcat cgtgtatttt ttttgagcgt tgtttgccct ttgcttttgt
    10921 tatttttttt gccgtcgtta aatttttttt attgttttcg actacttttg tatcttgaat
    10981 ttttttgatg atgagcgtgt cgtttttgca cgtcacaaaa atatcgttcc ccgcttgaac
    11041 gcgctcatcg atgattgcat cggtgactaa atcacgcagc gtgtcttgga aaaggcgcgc
    11101 catcggtcgg gcgcccataa cagggtcgta tcctttttgg gcgagatgtg cgcgcgccgc
    11161 cgcatcgaga tgcacttcaa tttctttttc tttgatttgt tcgttgagga gtgccaagtt
    11221 cttatcgaca attttttcca gtacggtatc acccaagcgg tcgaacacca ccacgtcgtc
    11281 aaggcggttc aaaaattcgg gactaaaata tgttttaagc gccgacttta tatgccctgc
    11341 acctttggct ttgtcttcaa agccgatgcg cgcggtactc agttcagacg accccgcgtt
    11401 cgaggtcatg atgagcaaca catggcggaa atcggctttt ttgccagtcg cgtcggtgat
    11461 ggtcgcgtag tccataattt gcaaaaggct attgaaaata tcaatgtgcg ctttctcaat
    11521 ttcatcgagg agcaacaccg cacgcggatg cttgcgaatt gcgtcggtga gttgcgcgcc
    11581 ttggtcaaat cccacgtaac cgggaggcga acccaaaagc cgtgagatag aatgtgcttc
    11641 catgtattcg ctcatgtcaa agcggatgag ttccacatcc aactcttcgg cgatcgcgcg
    11701 tgtgagttcg gttttgcctg taccggtggg gcctgtgaaa agaaaactac cgatgggttt
    11761 ttgtttattg cccaagcctg cgcgatggcg tttcaccgcg cgtgcaatct tttcaatcgc
    11821 tgcatcttga ccaaaaattc gttttttcaa attttcggca atcgacttga gcgatgcgct
    11881 ttcggcatta ctatattttt caagattaac gtgcgccaag cgcgacacga tcccgcgtaa
    11941 atcatcctcg gtgacttcgg tttgattggg tttgtagatg tgcaatgacg cgcccacttc
    12001 gtccaccacg tcgatagctt tatcgggcaa ataacgttcg ttgatatgtt ccgcagaaag
    12061 atcaactgca cggcgcaata tcatatcgct atacgtgaca cggtgataat cttcgtaaag
    12121 ttttctgagt cccaaaagaa tggcatacgc atcgtcgcgc gacggttcgt tgatatcaat
    12181 tttttgaaaa cgacgcgaga gagcgcggtc tttttcaaaa tagcggcggt actcttcgtg
    12241 cgtggtggcg ccgacgcagc gcatatcgcc acgggtgagc actggcttca acatattcgc
    12301 cgcgtccata ctgtcgccgc gcgttgcgca tacaccgaca atctggtgga tttcatcgat
    12361 gtataaaatg actttctttt ttgcggcgag cgcagccata atcatttgta cccgctcttc
    12421 aaagtcgcca cggaaacgag tgccggcgac gagcgaagag acatctaatg aaaaaacctg
    12481 gtagcctttg atacgatcgg ggactttatc ctcgacaagg cgctgcgcca aaccacgcat
    12541 gattgcggtt ttacccaccc ctggttcgcc aatgtgcaac gcgttgttct tgttacgtcg
    12601 acaaaggatt tccatcgtac ggttgagttc atcttcgcgg ccaatgagct tgtcgtattt
    12661 acctgctgct gcctcggcgg tcatgtcgac ggtaaactgc ttcaaaattt tatcggggtc
    12721 accttgcttt ttcgatggcg ccatattgtc gtcgtgcaca tcgccatcgg gtattgtcat
    12781 atcttcttca aaatcaccca acggatcggg attgattcca tgtgtgatat accgcaatac
    12841 tttagtacgg gtgatgcctt gtttttccaa aaaataagca gcgtatgaat cgtgctggcg
    12901 gaaaataccg tcaaaaatat cggcgataga aatttcgcgg ttgttgttca agataaggcg
    12961 caccaacagc atttcacgaa aattggagag tgccgccgat tcggtaattt cattgattgc
    13021 gtctaaatct tggtcgtacg tttggatgtg cccgatgagg tctttacgta gagcatgtat
    13081 atcgcaatgt aaatcggtta aaatatccaa gccaatttca tcttctgtga gtgcgagtaa
    13141 gagatgttca aaagtgatga tagagttctt gtaatcccgc gcaatatcaa acgcccgctg
    13201 gtggattttt tctactgagt ttgccatttt attccggttc tgttttacac gccaggggga
    13261 atccctcttg ctcagcgtgc gccatcactt gtttagattt tgtttcggca acttcgcgtg
    13321 ggtacactcc tgcaatgcca ctaccattgt tatgaacgtc taacatgacc cgttttgctt
    13381 cgtcttctcg cttttggaag tacttttgta aaatatagac gacaaactcc attgtagtat
    13441 aattatcgtt caacaaaatt actttgtaga gtttcggttt ttttatctgc gttaatacat
    13501 cagtatcgtc atcgtgccgg atgctcgtcg acataattcc aatatagtat aaccttcaca
    13561 agaaatcaaa aatatcttga aaagccgact ttcttttagg ttatgactgc catggataag
    13621 gtgcagataa aagaaattgc cgccggccaa gctgtcgcat acctgcacgg ccgcttagac
    13681 gttgcggttt ccagtgaaat tgaagaagcg ctgctaaacg ccattgattc taaaggtata
    13741 aaacgactgg tgctcaatat gtctgatgtt gaatacatgt cgtcttcagg ttttcgggtg
    13801 atgattgcaa tgctgcgcaa gttgaaagac ttacaaggct cgctcaaaat ttgctgcgtg
    13861 agtcccgccg ttaaacgaat tttcgacgtg attgaactca ccacactttt cgacatgtac
    13921 gacaccgacg aagccgcact ggcctaagtg ggtgcatgaa acaggttttc cttctttcaa
    13981 agacgagctt ttgtattact ttttttttgt gctttttgcc tcattgtagt tccgaggcaa
    14041 aaaataaagc gctctcgcac tcaaacgacc acgaaaccaa taaaaactta tctattagca
    14101 acgaattttt agcgggtaaa ttttcacctg caaaaaatca aaactttatc gaaatatcat
    14161 cgcagtattc atcgcgcaaa caatacatcg acaagcgcac tttcgccgcg tttgagaaaa
    14221 tggcgatagc agctcaaaaa gacggctttg acctcaccat catttccgct acacgcaact
    14281 tccataccca aaaaattatc tgggatgaaa aatttaacgg taagcgcaaa gtgggcggcg
    14341 aaaacattgc acaaacaatc cccaacgaaa aaaaacgcgc cttacgtatt ttagagtttt
    14401 catcgatgcc cggcacatcg cgccaccact ggggtaccga catcgatttg catgagaaaa
    14461 aaataaaagc ccctgcactc tataactcga cgttcaaaag cgggcgcggc aaagcgttgc
    14521 acgattggct cacaaaaaac gcaccgaaat tcgatttttg ccaaccgtac accggcgatc
    14581 ccgccacacg caacggtaac tcattccaac atggatacag agaagaaagc tggcattggt
    14641 catacacccc tctctcgaag ttgtatttac aacaatacac aaaacgtgca aaagcattaa
    14701 aaccttcggg ctatgcgggc gacaatgttg ccgcacactt ttatttagat ttcgtacaaa
    14761 atgtagatcc cacttgtagg tgaatatggc tattgaaaaa tcgttaacgc taccctgtgg
    14821 cagcacatta aaaaacagat tagcaaaatc ggcgatgagt gaaaacttgg cggtcaacgg
    14881 tgtacctgga gacaaactca tccgtctcta tgaaaaatgg ggacagggta acatcggcct
    14941 cgtcatcacc ggcaacatca tggtcgacgg gagcgagttg ggcgaaccca acaacatggt
    15001 tttagaaaat gatcattcgt tagacgcata ccgtaaaatg actacggcag ccaaaaagca
    15061 cggttcacaa atttgggcac aaatcaacca cccgggccga cagtctcccg ccgcgatttc
    15121 caaaacgacg gttgctccct ctgcggtgaa actcaaaatt gccaacattg ctttccgcac
    15181 cccacacgaa cttaccgacg cagaaattct ggatattatt caaaaatttg gccgcacggc
    15241 agaactttgt aaagaagcgg gattcgacgg cgtacaactc cacggcgcac acggctacct
    15301 caccagccaa ttcttgtccc cgctttccaa tcaacgcacc gatcgttggg gtggctcaat
    15361 tgaaaaccga atgcgttttc ccatcgaagt tatgcgcgcg atgcgcaaag cggtggggca
    15421 taagtacccc attgggatta aaatcaactc ggctgatttt cagcgcggcg gctttgatga
    15481 agatgacgcg gtcgcggtgg tgaaagcgct cgaagccgaa ggggtcgacc tcatcgaaat
    15541 ttcgggaggc acgtatgaag cggcggtgat gatgcgcgga gcacaggagg tgccagggtc
    15601 gacgcgcgac acgatgtcgc tccaagcggc gaccggtgcg caggtgcgtg cctcgaccgc
    15661 cgcgcgtgaa gcgtactttc ttccctttgc agaaaaaatt aaaaagtcgg tcaaaactcc
    15721 catcatgctc accggtggct ttcgcaccag tcttgcaatg aaccaagcta tcgaaggcgg
    15781 aattgtcgat gttgtgggta tcgcacgccc tctcgcgatg gaacctttag tttgtatccg
    15841 tcttttgcgc gatgcgaacg cacgcattga aatgcaacct gtcaaaaccg ggattgcaat
    15901 tttagaccgc atgggtgttt tagaaatcgg atattattcg ttacagctta aacgcatcgg
    15961 tgaaggtaaa gatcccgata aaaatttaag cccacttttg gcgttgccgc tcttggggct
    16021 caattactca tcgggtattt tatcgcggtt gttgaatcgg gcttcgtgat tgacactctg
    16081 ctgtcacttc caaatccaaa acgcagtagt ggaacctttt tatgaaattt accaaaatgc
    16141 atgggcttgg caacgacttt attgttgtcg atgaacgaac ccaaacaatc aacgctgatt
    16201 ttgtctctct tgcaaaaaag ctctgcgacc gacaccaagg catcggcgcc gacgggctca
    16261 ttcgcgtgct cccttcgaca aagggcgact tcaaaatgga gattatcaac tccgatggct
    16321 cgattcccga aatgtgcggt aatggtatcc gttgcttcgc taaatttgtt ttcgacaacg
    16381 aagtcaatcc caagaaagaa tacaaaattg aaaccggcgc gggtgtttta accccctcgc
    16441 ttgaaatcaa aaacggcaaa gtgattggcg tcaccgtcga catgggcgag ccgattttaa
    16501 caccggctaa aatacccatg ctggggtacg atagccactt aaaccaaaaa attaccgctg
    16561 gtggagtatc ttacaccttc actgccgtct cgatgggtaa cccgcatatt gtcatttttg
    16621 aaccggattt atctcatgta gaactatcgc acgtagggcc ttttttagag cgccacgccg
    16681 ctttccccgc acgcaccaac gtgcattttg tgagtatcgt ttcccccact gaaatacgcg
    16741 tccgccattg ggagcgtggc gccggtgaaa caaaggcgtg cggtaccggt gcatgcgcgt
    16801 gcgttgttgc aggttcgctc acagatcgca ctgaacgcaa cgtgttggtg cacgtgccgg
    16861 gtggcacact aaaaataaat tgggatgaaa ctaccaacca tgtttttatg cagggtgccg
    16921 ctgaatcggt ttaccaaggc gagctatttt aaagcaaaac aaaatttaca ttaaggaaaa
    16981 aatatgagtc ctctcacaaa agcaatccgc gcaaaaagga tttcaaacac ccctccatac
    17041 ctgtttgcag aaatcgacaa gaaaaaggca gcagcgattc aaaaaggaat taacttaatt
    17101 agttttggta tcggcgatcc cgatttgcct acaccaaagc acatcgtcga tgcaatgcac
    17161 gcagccatcg acgatgccaa aacgcatgac tatccccctt acgagggcac taaggaattt
    17221 cgcgaagcgg tggcggtgtg gatgtataaa cgtttcggcg taagcatcga ccctgttaaa
    17281 gaagcgatgg cgctcatcgg ttcaaaagaa gcgattgcgc atatacacac tgcgctcgtc
    17341 gataaaaacg acgtgtgctt gattccttca ccgtcgtatc cggtgtatcg agtagcgaca
    17401 attttttcag aaggcattcc gtacaatatg cctctgaaag aagcgaatgg ttttttaccc
    17461 gacttaaaaa aaattccggc ggatgtactg gcgaaagcaa aattaatttg ggtgaattat
    17521 cccaacaacc ccactggcgc agtgtgcgat ttaagtttct acgaagagct ggtggcgttt
    17581 gctaaaaaaa acaatatcat tatttgtagc gacaacgctt atagcgaaat gacttacgat
    17641 ggttttatcg cgccttcgat tttgcaaata cccggcgcaa aagacgtggc tattgagttt
    17701 ttatcgctct cgaaagcgta caatatgaca ggctggcggt taggtatggc ggtggggaat
    17761 gaagagctca tcgcggcgtt gggcattgta aaaacgaata ccgactctgg ccagtttaag
    17821 gcaattcaaa aagcgggggc aatggctctc accgcaccgc aggagtcaat ccgtgaaatg
    17881 aacgccatct accaaaaacg ccgcgatgcg atggtaggag gactcaattc gttggggtgg
    17941 aatattcaac ccatcaaagg gacattttac atttgggcta aaataccgcc gcgctttaac
    18001 tcctccgccg atttttgtgg ccacgtttta gacgaaaccg gtattgtgct cgtaccgggg
    18061 aatggttacg gcgatgaagg cgagggatac tttcgtgcag caatcacggt gagcgttgaa
    18121 aaaattgaag agggtatccg ccgcttaaaa gaaaaaaaga ttacattcta atgctttaat
    18181 aaatcctaca aaacttatag acaaactcac tacttctcag agaatcgaaa gtatgagaac
    18241 gattctgtta tttttggtcg tagcatgtgt cgtgggtgtc gttcatgcta atccgattgc
    18301 ttctaattgc acgttcaaag gacacaagct atacggaaaa gttaaaatag tcgaatcctt
    18361 ccctgatatt aaaataaaga ttgtggagtc gttccccgac ttaaaagtta aacaagtttc
    18421 cagttttcct gaccgttgtg gtaaatggca attcgtcgaa tccttccctg acgtaaaaat
    18481 aaaatttgtt gaatcattcc ccgatataaa gataaaatac gtggagtctt tccctgggat
    18541 aaactaagca tgaaaaagaa aatttttgta ataattttaa tcctactcgt cgctcttgcc
    18601 gctggtgtcg tttcacaaat tcgcttcgac atccccgctg ccgagatcga agcgaaatat
    18661 aaattgcccg aatcaaaatt tatcgacgtg aatgggctgc gtacacacta taccgacgaa
    18721 ggcgtaaaga agggctcgca cgaaacagta gtgttgctac acggcacagc ggcgagtcta
    18781 aacacttggc aagcatggat gcccgaactt aaaaaacatt tccgcgtggt gcgcgtcgat
    18841 ttacctgcgt ttggtttaac aggcccttcg ccaaagcgtg attactctat caaagcgtat
    18901 gtaaaatttt tggaagattt tctctccact ttgaaaatta aacaaattta tttagtgggc
    18961 aactctctcg gtggtgaaat tgcatggcac tatgccgctg aacaccccga cgatgtgtat
    19021 aagcttatac tcatcgatag cgcagggctt caacgcaaag gaaaaatgcc gttagcgttt
    19081 tggttagcgc gcaaaaaata tctgcaaggt ttggttaagt attttagccc acgattctta
    19141 ataaaaaaaa gccttaaaga cgtttacttc gataaatcca aagtcaccga cgcacttgtc
    19201 gatcgctact tcctgatgac gctgcgtgaa gggaaccgcc aagcgtttat tgatcgcgcc
    19261 aaccagattg aaagcgcaac cgatgacgat ctacggcaag tgcgcagccc cacgcttatt
    19321 ctgtggggta gacacgatgc gtggattccc gtcgaacaag cagaaatttt tcgtaaaaaa
    19381 ataatgattc ctcaacttaa aatatatgag aatgcgggtc atgtacctca agaagaaata
    19441 cccgacgaaa cccttgtcga tgtgataaaa tttttagatt aaaaatcgtg caaaccctct
    19501 atgaaaaaaa acaactacgc gccactctcg acaagaatga cgtatgccgc gtttatctaa
    19561 acaatccggc acaactcaac gcgctcaaca ccaccatggc ggaaggcgcc gttgagcttg
    19621 ctaccatcat taaagcgtca ccagcgcggg ttgtggtatt ttccggtgaa ggacgcgcat
    19681 tttctgcggg cggtgatttg gattatcttt tgcatttaac gacgctaccc aaagaagaat
    19741 cggcggcggc aatgttaaag ttttatcaat cgtatctgac gctctttact ctgccattag
    19801 caacgatcgc tcatatcaac ggcccttgtg tgggtgcggg gttttgcctt gcgcttgcct
    19861 gcgatttacg ctacgtcgtt tctggaacta aagtcggcat gaactttgtc cgcattgggt
    19921 tgaatccagg gatggcagcc gaagcatggg ctgtgcgcag cgatgaagta ttgttgcggg
    19981 agatgctcct tacaggaaaa atttattcat catccgaacc gcgtttaaga acacttttta
    20041 atttagtggc gtctcccgag gaaatacgaa ccgctgtgga agataccgcc acacaaatta
    20101 ccagcgcatc gccgcaatcc atccggctgt cgttgccgct tttacgcaac gcgcacttaa
    20161 cgcgcgaagc agtgatgcac gccgaagccg aaggccaagg tatttcaatg agtaccggag
    20221 aaataaaaga agcggtggcc gctcaacgcg ctggaaaaat ttttagattt aatttataga
    20281 tgaaattctt ggccgtcgaa cgcgggttcg gcttgccctt gccaacgcgc taaaagttct
    20341 tcgtgcatgt attgatgcga aagatggccg atgacgccgc gctttgcatt aaatttttta
    20401 aaaagttcaa gtgcctcctg ctgctgaatg tgcgaaacgt gggggtgatc gatgggcgaa
    20461 cctaaataga gaatatctaa gttatataaa tacttttcat cgttgtggtg tatctctttg
    20521 aagtcggtga gataaccgaa ttttttattc acgacaaatc cggtgctcaa cacacccgac
    20581 tgcggaatgt gaaccaaacg aatcggctga aattccatac ctgcaatttc aaccgcacga
    20641 tagccgtcct cttcgtcgcc tgggtataaa tgcaagccga tgcgcggcac gttggtataa
    20701 atagccgagt ttcgtatata aggatagcgc gttagaatta cttcgtgggt ttgcggattc
    20761 gcgtagcact taagctcgtc tttgctgtca aacgtaaacg gccgtaaatc gggcaatcca
    20821 tccaagtgat caaaatgaaa atgggtgtat aacactgcgt tgaccgtcgt taattttttg
    20881 cgcagcattt gctcgcgcaa atcggggcca gaatcgacaa gaacattacc ttctttgctt
    20941 tgaaaaaaga gcgatgcacg gaggcgtttg tcgcgcgtgt catttgaggt acacacttta
    21001 cacgggcaag taatgattgg cacccctgtt gaggtgccac tacctaaaac aacaactttc
    21061 aatattaact ataaaagttt gtctatatat tttttgatcg catcgtacga acgttcttgc
    21121 tggtacagta cgccattgat gaaaatagtg ggagttcccc tcacaccaat tttttgaccg
    21181 taacgcatat cgtcttcaat gctggcgcgt actacgccat ctttgtcggc aatacattgt
    21241 ttgtatgcgt tcatgttaac accaatggca gcggcaattt tatcgagttg ggtagtcgat
    21301 aagtccaacg atcgtccttg gtatggaatt tcaaaggctt tcttttggaa ctcaaagtat
    21361 ttattttgtt tacctgcaca ttcggcggcg atgtgtgttg gcattgcttg tgcgtgaaaa
    21421 tctaaaggaa agttgcggaa cacccattta actttatttg aatattctgc tttcaaacgc
    21481 tgtacatcag gctgcatttg gcggcaataa gggcattcaa aatctgaaaa ttcagtgatg
    21541 acaacttttg catcactttt accccagaac ggtttaccat cattgacaag attgagtttg
    21601 ggcgcctcgg gttcggttaa agtaaatttg aaattatatt gcttgaaaag ttcagcggtt
    21661 aatccgtcgc gcgcgctttt ttcttgatta tttaataatt gttggcggat gcgatcttta
    21721 atggtttcaa acggcatgtt gatgcggtgt ttattcattt cataaaattg tttcacgctt
    21781 gcatcggtaa caggcgcacc acggcgtttc gctaactctg tctccacata tttttcaacg
    21841 gtgagatttt gttttttagc ttcggctgca aaaattttat cgcgtgcgtt ttcgtacgcc
    21901 gcttgttgtc gtatctcaaa ttctttttgg cggatttcaa aaaattcctg cgcattgtct
    21961 ttttcaacgt ctttttgcgt aattttacca ccatcaaacg tggccaacac ttcgtcttgt
    22021 ggattaccac cgcatactgt ggttgtaaat actgccgcca tgataacaca acagcttaat
    22081 aattttctca tacgtacatg cagccttttc aagagtcgcc gaaaagcact tttatctatt
    22141 tagaatataa aagtataatt atacacaatt ttacacaaaa agcaatggtt ttacacaaaa
    22201 ttttcggcga tttattttta ttaaaaattt tcttaacaag agtgaagaaa tccacacgtt
    22261 cattttttaa taaaataata aatcttcttt aattaaattt gataaacaac tttatccact
    22321 tttccacact attattatta ttactatata taagtattat ataatataat aaataagaga
    22381 gtggaaatct tttatgagtt gacccctttt attaaagctc taatttgtta tttatgacaa
    22441 caacccatca agcaggattt cgatatcgtt ggataggcat cggcgacctc aatgcgtttt
    22501 ttggtcttat gctcgataac gtgacaaacc ttgttttcct tgcacaaatt ttaattcttg
    22561 tttttggatt tcccgccaac ttagtatacg gcaaaatgtt tcctggtacg gcgtttggtg
    22621 tgcttgtggg cgatctcatc tataccgcaa tggcgtttag acttgcaaag cgcacccaaa
    22681 acccaaacgt taccgcgatg cctctagggc ttgattcgcc ttcgacgatt ggtattgcct
    22741 ttgcagtcat cggccctgca ttcattgctg cgaaaaattc aggactcgac ccgcaagctg
    22801 ccggacttct cgcgtggaaa gttggtatgg caacgctggt aattatcggc gtagtaaaat
    22861 ttattctttc tttttttggg gattttgttg agaaattgat acccaccgca ggacttttag
    22921 gttcacttgc gggtgtcggt attgcactct taggactact cccattagaa catgtttttg
    22981 gtaccccagt agcagggctt gtggcgttgg gtattattat ctacgcactg gtggcaaaag
    23041 tgcgattgcc ttttagtata cccagcgtgt ttctttcggt gatcgccggt acagtcattt
    23101 attacattct ctctgcgctc cactttgtac atccacctga aatatcactt aaatttatta
    23161 ttgcattccc catgccaacg cttgaatttt taggcggctt caaagaagct tttgcatatt
    23221 tacccgtcgc gatcccgttt gcactcctta ctgtaattgg tggcatcaac gtgacggcga
    23281 gtgcacgtct tgcgggtgat tcgtaccgca ccagcagtat tcttttaacc gaagcggtag
    23341 ccacgttggc tgctggagtt accggcggcg tcgcgcaatc gacaccgtat attggccatc
    23401 ctgcatacaa ggcaatggga gggcgtgccg gttacacgct agccacagga ctttttatcg
    23461 gtttaggtgg cgtgttagga tttatcagtt ttctggcgca actgttgcct caatccgcgt
    23521 tggcgcccat tctcattttt gtcggtatcg atattgtgga gcaatcgttc cacgcaactc
    23581 caaaacggca cgcgatggcg gtggcgatgg caattttgcc ggcaatgcct tatttagcta
    23641 aaataaaaat gcaagggctt gtttttcaaa ttcaaacatt atacgcttca ctcgcacagg
    23701 cgtctgcgga aacagcggct aaacttaaac ccacgttaga tttcttcggt agcgcatttc
    23761 aagagtcgtg gtttacaatt gtcgcgttag ggaatggttt tattgtcact gcgatgatat
    23821 ggggtgcgtt caccgccaag ctcatcgacc gtgaatttta caaagcggca gcgtaccttt
    23881 ttgtcggtgc gtttttatcg ctttttggtc ttatgcattc gattaaaatt gatggtgata
    23941 tgtacttacc atggagtatc accgtgaatg catccggccc aacgccttat agcattgcag
    24001 cggcatacat aactttagcg atagtgataa ttatgctttc gtactcgaaa ggggctaaag
    24061 aagcgaaagc ggcaaaactg gatttagatt gataacgagc ttcggggata gattaacgtc
    24121 gcttcggtct ctttattttt tgcttcaatt ttcatgtgtt caaaagggtc gagtcccaac
    24181 tggtaaaatc cttggtcaac catggcgatg ccaaaccccg ccgattcggt ggtatccata
    24241 ttatcataac taaaaaactc gccacctttg ccttgtttat gcagcgcggc atggcgtatg
    24301 cggctatttt gaatccgctc taaatcgcgt tgtaacatgg gaacattatt caaaacttga
    24361 atgcggattt cgtctggagt tgcatgaatc tctaaaagca catcggcgtt aatcgaacga
    24421 attaactctc gaaactttgc aatgagttga atttgatcgt cgctcatttt tttactcatg
    24481 cctgcgcggt acttttcttc taaattaaaa acattttgta cttcgccgcg caacacatcg
    24541 ggcaagatgc gggtcgctgc aaactcgcgt aaagcacgtt cgttgagaat caattgtaaa
    24601 aaatcttcaa tttccgtcat ctcaaaacgg tttaattctt tacgaatttc ttctcgaaac
    24661 gcgacaaatt ttatattcgc tttgagggcg ttaaatacta attcgacaat cgcataatga
    24721 atgggcaaga catgcttttt atcaagactt tctttttcaa aaaattctaa aagaacgctt
    24781 tgaatagcct tacgcccttc tttagtgagt gcgttgattt gtaatcgaac cgctttctgc
    24841 gaattgaggc gcgagacggt tctttcaacg agtctgttgg taggcataat tatagtatcg
    24901 atatttttag gttgcgcagt ttaatgactt atatcaattt catcgccgcg ttgacgatga
    24961 aattgatact aaaattgtta tcgtttgagt cccaaaagct cttgcagcat ttgatcggct
    25021 gtcgtgatgc tacgcgaatt agcttgaaaa ccacgttgcg tcacaatcat gtcggtgaac
    25081 tggtcggcca agtcaacgtt tgacatttct aaaaatccag cgttgatacc acctaatcct
    25141 tgaataccag aggtacccac taatgcggcg ccagagttga tcgattctac aaagttattt
    25201 tgccccgatt tggtaagccc ctctggattt gcaaagtttg ccaatgccac ctgccccaac
    25261 gtgttgcgcg caccattcga gtaaacacca gtgatgacac cggtgtcgtt gatttcaaac
    25321 gcttccatgt aacccatagt gtagccgtct tgttcgaccg ccttggtggt gaacgcggat
    25381 gagaactggg tgatgccatc gacttgaccg gcttcgccta aacgtagttc gatgtttttc
    25441 accgcgggat ttccggctaa acgaaagcta atgttcgcgg ttaaattatc ttggttacgc
    25501 acatccgcac cgtcagaaac cgatgtcaat cgtccgtcgg gtgaaaatcc caacgtcatg
    25561 cgtgtgttgc ccgccacttg tgtgttttgg ccgttagaac ccgccacgtc gatggataac
    25621 tgttcagcgc cttgcatcgc caccgacgcc gtccacttat tcgtgtctga tttccataaa
    25681 tacattttca cagtgtgttc aacgccttga tcgtcgaaca cgttgatgct ggtgatgtga
    25741 ccacggcgtt tagagggatc ggcgtccata atcattttca cgcggtcggc gtcggatgcg
    25801 ccagccggga ttgcctccac cgaagaattg agattacttc tgaaacgaac aaaactcgtt
    25861 tccttagccg gctctttttg ataaagaggc actttcaaat tctctgtatc ggcggcggtg
    25921 ttgacgccca ttttaccatc ggcggatttt gtggcattcc atccttgaag acgcataccg
    25981 ttcgcaggat ttacgagcca accttcttta tccacgtcaa aattaccgtt gcgcgtataa
    26041 aactttttac taccatcggc taagacaaaa aacccctcgc ccgaaattgc taaatcggta
    26101 ttcttagatg ttgtttgtag cgcgccttga tgcatgagtt tgtcgatcga agcggtcatc
    26161 gcgcctaaac ccacttgttt tggattgaca ccgcccacgt tttggcgggg ttctgaagcg
    26221 cccgacatga gctgtgaaat catatcttga aacgtgacgc gttcgctttt gaagccgttg
    26281 gtgttcacat tcgagatgtt attcccaata acatccattc gtacttggtg gtttttaagg
    26341 ccggacacac cggcataaag tgttcgcatc atttttcttg ttctcctttt gaaactttgc
    26401 gtgtcgcttc gactgtgctc ggcgacacaa ctttttgtgt ttgattttct tcgtatgctt
    26461 tcgccacctc tgaagtagag gttttaatag gggtgattga tgtttcacgt gaaacattgg
    26521 gcaccggctg tctcgacata ccgctttcgg cgccatttga caacggagag ggtcgctgtc
    26581 cgttgggtat gttcgcggac gaggggctaa cttccgttac tgaggtaata tcgtcgaact
    26641 ttgtgaatcg cccgccgact ttgagaagga catccccgtt gggttcgtaa atcgccgccg
    26701 tgacgatgcc ttgtagatgt ttttcggtga caaagtcttt gccttcgacc atttttccaa
    26761 tgagcgtgtt tgcttgcgca ttacgcaatt tattcattga gctggcgacg ttattcattt
    26821 gttcgagcga cgagaattgc gccatttgtg aaatgaattc acggtcgttc atggggttgg
    26881 tggggtcctg cttagacaac tgcgtcacca acaatttcaa gaaactattt ttgtcgagcg
    26941 cgtcgtaacc ggatttacgc acaagcggcg ttttgccgtt atgcgaaaca ggttgtcttt
    27001 ggcgcgaacg ctggaattca cgcaaacgcg tcacgtccgc gtcttgcgtt tgcacgttat
    27061 ttatatcagg cataaagacc gccattttta acaacttcgc ctgcgcttgc ttcgtaagct
    27121 gacgttgccg cacgcacgac taaatcttgg agtaacgcct cttcggtgcg cgacttctca
    27181 gggttggccg cttgttgatt accgttgcgc acctcgatgt tgacgcggtt taggtcaact
    27241 gactcgcctt ccttgttgtt gttttggttt aagaaatttt gtagctcgct ttttacttct
    27301 tcgttttcaa cgacgatttt tccacgaatt tcaccttcga ccatttttaa atcgatttcc
    27361 atacggccaa tttctcgtgg aaaaagcttc actgtaaatt gcgcgttttg gctatccgca
    27421 ataagaacgt gcgcgcgcgc cataacatcg gtgagctgac gcgagagggc agcaggagtt
    27481 gttgtgtttt caatcgctgt tcggtggatt gtctgtgttc tcaccgacga aacagaatcg
    27541 ctcaaacgaa tatctaccgg tgtttgatta tttttttcat gcgtaatcgg ctttaactct
    27601 accgtattta ccgttggttg tttttcaata acgtttgttg aaattgtttc acgtgaaaca
    27661 atatgcgcct ttttaccgtg catctctgct ttagagagga aagttgtgtt cttagcgttg
    27721 ttttgtggcg gtgtgtcgcc cgctttttgt acgcggttgt ttagcgcctc ggtggatacc
    27781 cattctttcg aggtagtttt actttgtggg gtatgcgatt tttgagaatc tttttttgca
    27841 ttcagctttt cttctacttt ttgtaaaaac gatacgccta gtttttcact taaacgccgt
    27901 aatccatcaa cgttgagtaa aagcgtgtcg ggggtttttt gcagttgcaa caatgccgtt
    27961 gagatagccg cgttttttat agtacctttg tgtgaatgga gtggaattgt atgcgataaa
    28021 tttaccgcca aagttgggtc ttctttttta atcgcaagat gcttttgtgc cgctttactt
    28081 acttgcgttg cagcgttttt acccaaaagc cccagacttt ccgaaagttg attttctggg
    28141 cgtgtgtttg tagacgcctt tgtcgtcgct ggggagtttt cgtttatacc cgttttttgt
    28201 gcgtgttgcg gtttttcatt cttgcttacg atgtcttcgt tggctctttg cgctgctgtg
    28261 ccgtcgacgg gagatgattc atatcccttt tcggcgggag ctgttgtcgt cgtcgtgttt
    28321 gccggttgag ttgctgcatt atgagcagcg tctgcaacca ttgcgatata gcttgaaaaa
    28381 tccataaccg acttatcgcc atttgcggct tgtgtttttg cattattttt gggtaatgct
    28441 gaaacgtggg tcggcgtaga attgagtata ttcagagaga gcatctgtat tttgatatat
    28501 cgggttcttc tcaaaaaaat cgagcggaat ttctaaaagg tttgtcgttt tttagaaaaa
    28561 atatctcaag cttttctaaa atgtgcacat aatgtatgtt tcaaaaagtg acataaaccc
    28621 ggaggcaatc cacatacttt tccacaaatg tggaaaaccc atgtggttta ctttgactct
    28681 tttggggtgt ttgaaaagat ggattgaata tctgcaggta tttctgctaa aagcggccat
    28741 ggtttgccag agacggggtg cgccagtgtg agtttttcac aatgcagcgc cgaccgtgca
    28801 atacgtagag aatcccaatc gtcatgcgtc atggtgtcgt cggcaaattt aaaatagaaa
    28861 tcgtgattta ttccgtaaag tttatcgccc acgacaggat atcccaaaga agagagagtt
    28921 gctcgaattt ggtgcatccg acctgtaaaa agttgcgctc tcaagagagt gagattgttg
    28981 ttagttaata acggcgtaaa gagcgtgtgg cagcgttgga tttccatagc tacagcgggt
    29041 ggattggtac tgaaataccg ttggcgcggt agacgcgagg tcggcaggcg gaaaataaac
    29101 ccttcggcgt cgataggggt atctacagag ccttcaaccg ctacgacgta ttctttcttt
    29161 aatgcccccg cgcgtaaaat ccggtggaac gccactgctg cagtgcggtt tttggcgagc
    29221 gccagcacgc cactcgtttc gcggtcgaga cgatgtagta agtagggagt agttaataaa
    29281 cgccgctcga caagtagcgt agtgatagtt tgggttttat agatacccga agggtgtacc
    29341 ggcaagccag cgggcttatc caccacgaag taatctttat cttcgtaaat ggtttcgatg
    29401 cgggtgttta cctcgggttc ctcgcgcatg gtatagctaa actcaatggt atctcccacg
    29461 ttgagtttgc gtgcctgttt tggcacaata ccgtttatac ggattgtttt ttcgtcgatg
    29521 cgcgtttgcc acttgttgcg ggaaaatttt gtaaaacggc gggttagaaa caggtcgagt
    29581 cggaggccac tttctgtatc gttaactaca atttgtctgc tttcgatgcg catgaatact
    29641 atctcgcaaa gtcgtttatg gtaccttcta cgacaatcaa aattaataaa atattgtctc
    29701 agcgagtcgg agctaggctc ttaattggta aagctcttgc cgcgctgttt cgctcctgcg
    29761 cagtttcgct atggtagaac tcaatcgtta cagtaaaact ctcacccaag atccatccca
    29821 gcccgcatcg caagcgatgt tctatggcat tggtttcaaa gacgaagatt tcagtaaagc
    29881 gcaagtcggc attgtgagca tggggtacga cggcaataca tgtaacatgc acctcaatgg
    29941 tctcgccgcc gaagcgaaaa aatcggtatg ggaaaacggt atggtcggtt tggttttcca
    30001 taccatcggc gtgagcgacg gcatggcgaa cggtaccgac ggcatgcgct tttcgttggt
    30061 aagccgcgat gtgattgccg actcgattga gaccgtctgt ggcgcacaat actacgacgc
    30121 actcatcgca atccccgggt gcgataaaaa tatgccgggt tcgctcatcg caatggggcg
    30181 tctcaaccgc cctgcgatta tgctctacgg cggcacgatt gcaccaggta aatataaagg
    30241 aaaagatctc aacatcgttt cttcgtttga agcattgggt gaaaaaattg cagggaaaat
    30301 tagcgacgaa gaattccacg aaattgtatg ccacagttgc ccgggcgcgg gcgcatgcgg
    30361 tggcatgtac actgcgaaca ccatggcagc ggcgattgaa tgcttaggca tgagtttacc
    30421 ttatagctcg agcaaccctg cgctcagccc cgagaaaatc ggtgagtgta aagaagtggg
    30481 cgctgcggtg aaaaacattc ttgaaaaaaa tattctacca cgcgatatca tgacaaaaaa
    30541 agcgtttgaa aacgcaatca ctctcattat ggtcattggc ggctcgacca acgcagtgat
    30601 ccacatcatt gcgatggcaa agagcgtggg catttctgtt actcaagatg attttcaacg
    30661 catcagcgac aaaacgccgc tcttggcgga ttttaaacca agcggtaaat acatgatgca
    30721 agacttgcac gagaaaggtg gaatccctgc ggtgatgaaa tatcttttga atcgtggtat
    30781 gcttcacggc gattgcctca ctgtcacagg aaagacgatc gcagaaaatt tgaaagacgt
    30841 tgctgagatt gatttcacca agcaagacat catttatccc attgaaaagc cattgaaggc
    30901 taccgggcac ctacaaattt tatacggcaa tttggccaaa gggggcagcg tggcaaaaat
    30961 caccggccat gaaggcgagc gcttcaccgg tcctgcgcgc gttttcg
//
LOCUS       DAOMPD010000002        22027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1016_length_22027_cov_3.524122, whole genome shotgun sequence.
ACCESSION   DAOMPD010000002 DAOMPD010000000
VERSION     DAOMPD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 22027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22027
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1016_length_22027_cov_3.524122"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..571)
                     /locus_tag="PLY93_00155"
     CDS             complement(92..571)
                     /locus_tag="PLY93_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67933.1"
                     /translation="MKKLIILLSACFFVVGVGARSNNKASQNLQKLLGLYQSDMVAFF
                     ASVQNIDNALPAFFCDVSFANDSQVENFLRQVSTSTPRGQMYSQVSYTEAVSINGIVR
                     AVSYNLTNDRGNVKFVKSANVNGQLLRAVYTLDPSTKKLKTATFDGKKAIRSNEYSL"
     gene            complement(636..2645)
                     /locus_tag="PLY93_00160"
     CDS             complement(636..2645)
                     /locus_tag="PLY93_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRP67934.1"
                     /translation="MLKTRLLNLSFTTKRVLQVTADIVLVWLSLALAFVLRVGWQPSI
                     DAMQVHLWLFVAAPAVVVLVFIPMGMYRAVIRYLGREALAGIAKAVTFSALLLALLIY
                     WYQGSRFNIATGASNIPRSFILNYWVLSFVFMGGLRLFMRYYFVGDWSTLGVNLMPFM
                     GKYAHLPKVAIYGAGSAGNQLVTALRMGKLMHPVAFIDDDVKLQRRTIAGLRVYPPTQ
                     IERLIADTEITQVLLALPSVGRSRRREIITSLEAYPLHVRTIPGFMDLASGRIAVQDI
                     QEVDIGDLLGRDPVAPNKELFEKCIKEQIVMVTGAGGSIGSELCRQILVNSPSALVLY
                     EHSEFNLYSVHQELARRIERAALPVRIIPILGSIRNAKRLREVMETYKVNTIYHAAAY
                     KHVPLVEHNIVEGVVNNTLGTLYTAQAAIAAQVRNFVLVSTDKAVRPTNIMGATKRVA
                     ELVLQALAKEKDIALFNKDTTVRYPNRTRFTMVRFGNVLGSSGSVIPLFREQIQNGGP
                     VTVTHPNITRYFMTIPEAAELVIQAGSMGKGGDVFVLDMGEPISIVDLARKMILLTGL
                     TIRSDENPKGDIAIEFTGLRPGEKLYEELLIGENVESTEHPKIMRANEKMHAWGELEE
                     YLLYFKQLTEQENTIQLRQLLREVVEDFNPQSDIVDLVYREKRTG"
     gene            complement(2649..3242)
                     /locus_tag="PLY93_00165"
     CDS             complement(2649..3242)
                     /locus_tag="PLY93_00165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRP67935.1"
                     /translation="MLKRSFDFLASLIGLILLCPLFVVIAIAVKISSAGPVFFRQLRV
                     GFAGKLFQIHKFRTMRIDAEKKGKLTIGKDSRVTRVGYFLRKTKLDELPQLLNVLKGD
                     MSLVGPRPEVPEFIEYYSPIAREKILSVRPGITDMASIEMVDENKILAEYENPHQAYI
                     EKILPLKEKYYLYYVEKQSLWFDIYLIFKTFIKILTR"
     gene            complement(3229..4398)
                     /locus_tag="PLY93_00170"
     CDS             complement(3229..4398)
                     /locus_tag="PLY93_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRP67936.1"
                     /translation="MTQPFLPFALPEIGDEEIHEVIDSLKSGWVTTGPKAKKFEENFT
                     EFLGDNRLQSIAVNSATSGLHLALEALGIGPGDEVVVPVYTFTATAEVVRYLGATPVF
                     VDVDPVTFNITAPDLKAAVTSKTKAVVPVHFAGLPCEMDEILQVAKEYKLYVVEDAAH
                     ALPSKYKGKLIGTLDSDFTVFSFYANKTMTTGEGGMVVTRNEDFAKRCRVMRLHGISR
                     DAFDRYTSTKPAWFYEVIAPGFKYNMPDIAAAIGLHQLKRLNGFHEKRVKMAQVYQKE
                     LAHESLILPFSGNNDKTHAWHLYPIRLSKEAKISREDFIVEMSKLGIGCSVHFIPLHL
                     QPYWRDTYRLNSEMYPHAQESYSQEVSIPLYTNMSDADQQRVIDAIKKVLHCAQT"
     gene            complement(4395..5627)
                     /locus_tag="PLY93_00175"
     CDS             complement(4395..5627)
                     /locus_tag="PLY93_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRP67937.1"
                     /translation="MNILLINHYAGSPQYGMEFRPYYMGREWQRMGHEVKILAGSYSH
                     IRSKQPEMSSGKHKDEIIDGISYRWYKTTVYTGNGIKRVLSMLVFLRHLKRDAKKIAQ
                     EFKPDLVIASSTYPMDIWPARRIAKLSKAKLVYEVHDLWPLSPIQLGNLPRWHPFIIW
                     VQMAEDYAYRHADKVVSMLPKTLEYMQSRGLAPKKFVYVPNGINLDEWKKPESLNNEV
                     LQIIKNLKKKKQPIVGYVGTHGLANALDTLLDAMKILRGRVQLLSVGTGPEKEKLIKR
                     VKDEEISNVIILPSIPKKEVPNLLQLIDIAYIGLKPEALFQYGISPNKLMDYMVSEKP
                     IVCAIKAGNDPVGEAGCGITVPPGDAQLVADAIVKLSKLSPTQRKTMGTKGKSFILKN
                     QTYPVLAKRFLAAMDGEL"
     gene            complement(5627..5833)
                     /locus_tag="PLY93_00180"
     CDS             complement(5627..5833)
                     /locus_tag="PLY93_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRP67938.1"
                     /translation="MPCVTLRDETEWVELVDAGWNRLAPPTDSHTVHDVFMSALGSQG
                     KSVEPYGAGDAAEKIVARLGKDLD"
     gene            complement(6147..>7000)
                     /gene="wecB"
                     /locus_tag="PLY93_00185"
     CDS             complement(6147..>7000)
                     /gene="wecB"
                     /locus_tag="PLY93_00185"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRP67939.1"
                     /translation="QQTGQMMAGIESIILSEKPDAVLVYGDTNSTLAGALAAAKLHIP
                     IAHVEAGLRSFNMQMPEEINRILTDQMSDILFCPTDAAVANLSAEGFAKKAVQVLKVG
                     DVMQDAALFFARQAIPPQKFNLADKFILATLHRAENTDNPERLASIVAALNKIHTEIA
                     PVVLPLHPRTRKIIQEKGLTLTANVINPIGYFEMIWLLQHCGLVLTDSGGVQKEAYFF
                     GKPCVTMRDQTEWIELVEVGANELVGADTQKIVDAVKRKNGQAIVDEAQLYGGGRAAA
                     EVVRHLV"
     assembly_gap    7001..7010
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7011..7271)
                     /locus_tag="PLY93_00190"
     CDS             complement(<7011..7271)
                     /locus_tag="PLY93_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRP67940.1"
                     /translation="MKKILTIIGARPQFIKASVVSRAIQQTDRLTEILLHTGQHFDAN
                     MSDVFFNQLGIPRPHYQLDIHGGNHGEMTGRMLIEIEKVLLRE"
     gene            complement(7680..8804)
                     /locus_tag="PLY93_00195"
     CDS             complement(7680..8804)
                     /locus_tag="PLY93_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP67941.1"
                     /translation="MKIALLSSASSIHTVQWAQWLNKAGHEIHIISQHPLTESLMQGI
                     TLHKIPYRGILGYFTMVPRVKKILKQIKPDIVNAHYASGYGTTARVVNYQPWLLSVWG
                     SDVYDFPYTSPLHKWLVKTNLYAANAVASTSVCMAKQTRIIAPKLKDIAITPFGVDIQ
                     IYRNVKPAFAHNNKQIVVGTVKTMASKYGIDILIEAFAILFKRFQKSDKNIADRLTLR
                     LVGDGPEIKQYKQQVERLKISNRVEFVGRVPHSQVPQELAKFDIFAALSRLDSESFGV
                     AVIEASATARPVVVSDVGGLPEVVLANKTGFIVPREDSQAAAVALEKLVLNPKLRQRV
                     GLAGRGHVEKTYNWPRCVEQMVKVYSGVVSSYKLAYNKAK"
     gene            complement(8801..9886)
                     /locus_tag="PLY93_00200"
     CDS             complement(8801..9886)
                     /locus_tag="PLY93_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67942.1"
                     /translation="MKKILFHHPLPLDPNAKSASGIRPQRMLQAFKDLGYEVELITGY
                     AKERKTAIRQIKKNIRNGVKYEFVYAESSTMPNILTEKHHLPTHPFIDCRFFQFCKKN
                     KILIGLFYRDIYWRFEEYRKALNMVKVLVAKLAYRFDLYVYQRTVYKFYLPSLEMAKY
                     IPTINQKKFRALPPGHASPVTQFKPVSTKLKLFYVGGFSSHYQMHVLFNVLKQKPTVE
                     LTLCTREDEWLKMRAEYPEPSANIKIIHKVGHEMERELLAADIAVLFVKPQEYREFAA
                     PVKLYEYIGFKKPILASKKTLAGKFVDNYRIGWAINYDENALIGLLNALEKHPDRIRR
                     KVDNLEKISTEHSWRARAKQVIQDLTQ"
     gene            complement(10129..12009)
                     /gene="asnB"
                     /locus_tag="PLY93_00205"
     CDS             complement(10129..12009)
                     /gene="asnB"
                     /locus_tag="PLY93_00205"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRP67943.1"
                     /translation="MCGITGGWWQSPEQAIQRLPAVLQKMKHRGPDDSGFEVYPLHKA
                     AVVLGHTRLSIIDLSNAGHQPMHSGDGTLAITFNGEIYNYRELRIELKALGHHFITDT
                     DTEVLLAAWREWGVNCLSRLAGMFAFAILDKNANTLTCVRDAFGIKPFFYATNSSDFS
                     FASELPALLELLPSKPQLNWQRAYDYLVHGDYDSTPETFYAELRHLMPGHYLQVNTLT
                     GKTDIPKRWWQPNITERGDWNFDDAVEQVREQFLQNIRLHLRSDVPLGAALSGGIDSS
                     AVVCAMRYVEPKMPIHTFSFIAEGSRVNEESWVDCVNTHVGAAVHKVRVTSQELAHDL
                     DDMIRTQGEPFGSTSIYAQYRVFQHAKANGITVTLDGQGADEMLAGYNGYPGPRLRSL
                     LETGHLIDAWRFHKEWARWPGRSRIQALKFVVSEMTQGSLHSLLRYMTGKSAEPSWLN
                     GSILQEHGVKLVKARIRPENNIAGRRVIDELALSLSHRGLVSLLRHGDRNAMRFSIES
                     RVPFLTLDMVNLLLSMPEKYLVSPQGETKHVFRAAMRGIVPDTILDRKDKIGFETPEK
                     NWLLSMAPTVREWLKTELDLPFLKQEKILQEFDAVVAGKKAFSWQVWRWINFGRWYVL
                     NF"
     gene            complement(12014..13333)
                     /locus_tag="PLY93_00210"
     CDS             complement(12014..13333)
                     /locus_tag="PLY93_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67944.1"
                     /translation="MHYSNKIFLGVTEIAGYYAGLQKGLCELGYQCNHYLYFSHPFKY
                     SQPSRSLLCNIIRMLIQLHNRLSHKNHFFELLKLLLFPSRLIFQALFFFSCIFKFDIF
                     IFGYCTSFFSPLPKIFSCFEFSELYLLKLLGRKIIFVMHGSDVRPPYMDGTYQGVDIK
                     TFVSLNKERKKRITRLEKLANYIVCDPAYAAYFTKSVINRSYIMCPASVIYKADDILQ
                     RTDNKAPFSIVHAPSAPSLKGTLEIQKAIQNLKRQGYRIRYTEIINQSNQKVIETILS
                     SDMVIDQLYSDIPLPGLASESALLGRAVVMGIYDQKFVLGQIDAKARPPVCYVYPESI
                     EEELLKILKKGRHFIFKQGKKLQKFAYQNITPLVVAKRFELIFENRIPGKWWYNPNAY
                     DYIFGCVITKKRLFSNIKKIINEFGISALQMNDKPQYLKKLLSRLKN"
     gene            complement(13351..14565)
                     /locus_tag="PLY93_00215"
     CDS             complement(13351..14565)
                     /locus_tag="PLY93_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67945.1"
                     /translation="MKKILSRIYYFSLFQFVIKGVILVVVLPFLIILIIISRYKRKPI
                     QIGFGPEPLINNLYHKRALEEFGFTAETFVNQVYFITDEFDIRVDKLPFPINILSNYL
                     LGFMVLWRYECLYIYYNGGPFAWTLLAKWEPLIYRFAKIKIHVTAYGGDVHDLRRVVN
                     LNFKRALDNDYPMFYLQQVKTEKQVARWSKYGTHISSGCDWVYYVPHWHTLMLGHFCI
                     DLAKIHFNSNESIPKKFNSKRPLRLFHAPNHKAIKGSNFFINAVKELREEGCAIELNL
                     KQGVPNSEIQESIQDCDVVLDQLIIGWYAMFSIEAMSHGKPVVCYLSEELLEFYTVAG
                     LITPHEVPIINASALNVKSIICDIYTGKIPLKNLGINGRKFVEKHHSLEVIGEKFAYI
                     NQLMDINPVVKK"
     gene            complement(14584..16398)
                     /gene="asnB"
                     /locus_tag="PLY93_00220"
     CDS             complement(14584..16398)
                     /gene="asnB"
                     /locus_tag="PLY93_00220"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRP67946.1"
                     /translation="MCGIAGLFGQVDGARHRVVNMTDQLYHRGPDDYGIYSEDALGVH
                     LGHRRLSILDLSTAGHQPMVHPESGNVICYNGEIYNYRDIRADLLKLGYTFHSNSDTE
                     VALKAYHAWGNKCVERFNGMWALVIFDKERQVIFGSRDRIGVKPLYYALSKEFLIIAS
                     EVKAILSTGLIHAKPDMEGMNEYFTFQNIISDRTLFAGVRMLEAGHNFEYQLREKDLQ
                     IKRYWDLVYAPQQLDEQEYVELIHEAFHKSLDRHLLSDVDIGATISGGMDSSAIIALA
                     SQKIKGMHTFTGYFDTSAIETNDRAVSEKDDARLISNMFGTTHHERLISPQDMIDNLP
                     QIVWYLEDPKVAMCYTFYTIAQKVSQRVKVNLSGTGGDEIFAGYPWRYHLFQNVESES
                     EFRNIYYNYWCRLLNDKERITLFKPQFANQIDFTRSRQVFNHIIDNAGNVSFLNKALY
                     FENKTFLHGMLMVEDKMGMAFSVETRVPFLDNELININVLIPDAYKYRNGVSKYILKR
                     AFDKLLPKEIIHKRKQGFTPPDQTWYRRELSDYLRNLLLGHKTVCHEYIEPKVIEKIL
                     NRHALGSDERMLIWSLAFFEGWCRVFLAKTEIPRSLQF"
     gene            complement(16407..>17034)
                     /locus_tag="PLY93_00225"
     CDS             complement(16407..>17034)
                     /locus_tag="PLY93_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRP67947.1"
                     /translation="EYKVRKFVHASTGSVYGEPTIFPTKEDHPLRPVSYYGVSKLAGE
                     RYVDVFHHLYGLDTTILRYFHVYGPRQESNEFGGVVSIFLRNIMENKPMTIFGHGDQM
                     RSFTWVKDLAKVNLAAAMNPKARGAVYNAASGIQVTINDLALRMKEMMQSGVEIIHGE
                     PLLGDIMFFDVSNHAVCTDLNVSFEKDFWGTLNRTLDEFRDYLSKVVQ"
     assembly_gap    17035..17044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17045..17367)
                     /locus_tag="PLY93_00230"
     CDS             complement(<17045..17367)
                     /locus_tag="PLY93_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP67948.1"
                     /translation="MDLKGRKAIVTGGAGFIGSHICEELLNAGAEVISIDDYSAGKER
                     NIEFMKANPKFKAYKKDVCDKDGWDEILTDCDTIFHNAASKKNICLINPQRDLDVNAG
                     GTLNLL"
     gene            complement(17406..18698)
                     /locus_tag="PLY93_00235"
     CDS             complement(17406..18698)
                     /locus_tag="PLY93_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP67949.1"
                     /translation="MKTVIKVENLSKSYRLGSISTGSLHKDLQSFWARTRGKEDPNRR
                     IGESSQHRANDDFFWALKDVSFEVQQGEVLGILGRNGAGKSTLLKILSRITAPTKGSV
                     KIRGRIASLLEVGTGFHPELTGRENVYLNGAILGMTRREIGRKFDEIVDFAEIEQFID
                     TPVKRYSSGMYVRLAFAVAAHLEPEILIVDEVLAVGDVQFQKKCLGKMGELRREGRTV
                     LFASHQMATLTNLCQRGILLENGRVSSDSEITSVIQTYNRSLASARITREAAHALGLK
                     ESQKAQFISAICRHGETQAEQQRYTLTDSVLIEIDYEILQDTDEVIIPNLHVINSLGI
                     LVFITWPFSSAPLTKGRYTAQCRIPDNLLNEDEYGINLYINSYTKHASGNEVKLENVI
                     NLLITDDKKLDNPKRYSVGARIGGVIHPDLPWRIQKIN"
     gene            complement(18706..19584)
                     /locus_tag="PLY93_00240"
     CDS             complement(18706..19584)
                     /locus_tag="PLY93_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP67950.1"
                     /translation="MSSSSSEEKWDIVIRSKKSWFSIDWRELWRYRDLISMFVRRDFV
                     AFYKQTILGPLWYLIQPLFTTMIFVVIFSRIASISTDGVPAVLFYLSGLVLWNYFSDC
                     LVSTSSTFLDNQNIFGKVYFPRLSIPISIIFSKLIKLAIQLVLLALIYLYFLFKGEAV
                     QPSPWLPLVLPMLIIQMAFLGLGTGILISSLTTKYRDLNYLVGFGVQLWMYATPIIYP
                     ISQIPEKYRILVNLNPAAQVVTNLRFILFGGSASTSLVPAIGNDLVISLVITLVILLL
                     GLFMFARVEKSFIDTI"
     gene            complement(19657..21384)
                     /locus_tag="PLY93_00245"
     CDS             complement(19657..21384)
                     /locus_tag="PLY93_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP67951.1"
                     /translation="MLVIVMATMELVGIASIGPFMALVGDMNLINTNATLQKVYALSG
                     LTSPKDFLFFSGLTVLFLLGFSALVSIVTTWRLSFYGFAVGTEMSNRLYRFYLQRDWL
                     FHVSSSSSQLINHVQSETSRVTHGILLPMLQLNARVALAVFIGVSLFVFNPWVAILGL
                     LVFSSGYFIVYKLVKNRLMRYDKNLSRASEIRYRLMIEGFGGIKDILMLQRGVTFTEE
                     FANQGVPVTRTQALISAIGQIPRYFMEFLAFCTMVSLILVLVRFYNGDLSTVLPLIAI
                     YGLAGFKLLPALQQIYASVVTMKGNAAAFHAIKDDLKSALLVHEGKMTLNSKKIDLVM
                     GSIQLKDVSFTYPTKSEPALIQANIIIPANSTVGLVGATGSGKSTAIDLVMALILPQK
                     GSLLVGGVPITVENREAWQRQIGFVPQSIFLTEGTIAENVAFGLRVSEIDAARVNRAL
                     QLAHLADFIATLPEGIQTKVGERGVRLSGGQRQRIGIARALYNDTKVLVFDEATSALD
                     GITEKIIMNAIDELSGERTIILIAHRLKTVMNSDIIYILEGGRVVDSGTYRELVDKNV
                     KFREMALNA"
     gene            complement(21650..>22027)
                     /locus_tag="PLY93_00250"
     CDS             complement(21650..>22027)
                     /locus_tag="PLY93_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP67952.1"
                     /translation="QAGQNFNSIGKLEVSQIALEELLQNALVHRDYSKNSPVRVMIFD
                     DRIEILSPGALPNSLTIENIKLGNAVVRNNLIVSYSSKMMYYRGFGSGITRALQAQPN
                     IELINDIEGEQFRVIIPRPEKQN"
BASE COUNT         6509 a         4962 c         4119 g         6417 t
ORIGIN      
        1 ccgatcgact tttaacgtcg ggcctcacag tgcctttgaa aatcggcagc gtaaaaccca
       61 acgcactcga cgtgactgtg gagtgattat tctataaaga gtattcgttc gaccgaatgg
      121 cttttttgcc gtcgaacgtt gccgttttta actttttggt gctggggtcg agcgtataga
      181 ctgcacgcaa caattgaccg ttgacatttg cactctttac aaacttcaca ttgcctctgt
      241 cgttggtgag gttatacgac acggcgcgca caatgccgtt gatcgacacc gcctcggtat
      301 aactcacctg gctatacatc tggccgcgcg gcgtagacgt agagacttgg cgcaaaaagt
      361 tttccacttg gctatcgttc gcaaacgaca catcgcaaaa aaatgccggt agcgcattgt
      421 cgatattctg cacactggca aaaaaagcga ccatatcgct ttggtaaagc cccaagagct
      481 tttgtaaatt ttggcttgct ttattattcg aacgagcgcc caccccaaca acaaaaaaac
      541 aagcactgag aagtatgatt aactttttca ttttccctcc atttgtttag cagaccctgc
      601 ttgggataaa atcgtttcaa ggggggatta tggagtcaac ctgtacgctt ttcacgatac
      661 accaagtcaa caatatcgct ttgggggtta aaatcctcaa ccacttcacg caaaagttga
      721 cgtagttgaa tggtgttttc ttgttccgtt agttgcttaa aatacaaaag gtattcctca
      781 agttctcccc aagcgtgcat tttttcgttg gctcgcatga ttttaggatg ttcggtgctc
      841 tctacatttt cgccgatgag taactcttcg taaagttttt cacccggcct aagaccggta
      901 aattcgatgg cgatatcgcc tttcggattt tcatctgagc gaatagttaa tcccgtcaat
      961 aagatcatct tccgggcaag atcgacaatg gatatcggtt ctcccatatc gagaacaaag
     1021 acatcgcccc ctttacccat cgaacctgct tgaataacca actctgccgc ctcggggatt
     1081 gtcataaaat aacgcgtgat attcgggtgt gtgaccgtta ccggcccgcc attttggatt
     1141 tgttcccgaa aaagcggaat caccgatccc gacgaaccca gcacattacc aaagcgcacc
     1201 atcgtaaagc gggtacggtt ggggtaacgc actgttgtat ctttattaaa aagtgcaata
     1261 tctttttctt tggccaatgc ttgtaaaaca agttctgcaa cccgttttgt cgctcccata
     1321 atgttggtgg gacgcactgc tttatcggtt gagaccagta cgaaattacg cacttgggct
     1381 gcgattgccg cttgcgcagt gtagagtgta cctagagtat tatttaccac gccctcgaca
     1441 atgttatgct ccaccagagg gacgtgctta tacgccgcag catgataaat agtgttcact
     1501 ttgtacgttt ccatcacttc acgcagccgc tttgcattgc ggattgaacc caaaataggg
     1561 attattctca ctggaagcgc tgcgcgttca atgcgccgcg ccagttcttg gtgcacgcta
     1621 tataaattaa attcgctatg ctcgtaaagc accaacgcgc tgggagaatt gaccaaaatt
     1681 tggcggcata actccgaacc aatggagcca cccgctccgg tcaccataac gatttgttct
     1741 ttgatacatt tctcaaataa ttctttattc ggcgcgacag gatcacgccc taataaatcg
     1801 ccgatatcca cttcttggat atcttgcacc gcaattcgtc cactggctaa atccataaaa
     1861 ccagggattg tacgcacatg caagggatat gcctcaagag aagtaatgat ttcacggcgg
     1921 cgagagcgac cgacggaagg cagcgccaat aacacctgag taatttctgt atccgcaatg
     1981 agcctttcta tttgcgttgg gggatagaca cgaagcccgg caatcgtgcg tctttgcaat
     2041 tttacatcat cgtcaataaa cgcgacgggg tgcatcaact tccccatacg caacgctgtg
     2101 accagttggt ttcccgcaga gcccgcaccg tagatcgcca ctttgggtaa gtgtgcatat
     2161 ttacccataa aaggcatgag atttacaccc agcgtgctcc aatcgcccac aaaataataa
     2221 cgcatgaaaa ggcgcaatcc ccccatgaaa acaaaactca gcacccagta gttcaaaatg
     2281 aacgagcgcg gtatattcga cgcgcctgtc gctatattaa accgcgaacc ttgataccaa
     2341 taaataagca gagccaaaag aagagctgaa aaagtgaccg ccttcgcaat acccgcgagc
     2401 gcttcgcgcc ccaagtaacg tattaccgca cggtacatac ccatcggtat aaataccaga
     2461 acgactaccg ccggcgcagc gacaaaaagc cacagatgca cttgcattgc gtctatcgac
     2521 ggctgccacc ccacacgcag cacgaacgcc aacgccaacg aaagccatac caatacaata
     2581 tcggctgtga cttgcaaaac gcgcttggtt gtgaaggaga gatttaagag gcgggttttt
     2641 agcatgcgtt agcgggtgag gattttaata aaggttttga agataagata tatatcaaac
     2701 caaagacttt gcttttctac atagtaaaga taatactttt ctttaagtgg taaaattttt
     2761 tcaatatatg cttggtgtgg attctcatac tctgctaaaa ttttattttc gtcgaccatt
     2821 tcaatcgacg ccatatcggt aatgccgggt ctaacagaaa gaattttctc acgagctatc
     2881 ggcgagtaat attctataaa ttcagggacc tccggtcttg ggcctacaag gctcatgtca
     2941 cctttgagaa cattaagaag ctggggtaat tcgtctaact tggttttacg taaaaaatat
     3001 cccactcgcg taacccgtga gtccttaccg attgttaact tgcctttttt ttcggcatcg
     3061 atacgcatgg tgcgaaattt atgaatttga aataatttac cagcaaaccc cacacgcaac
     3121 tgtctaaaaa aaactggtcc tgccgaactt attttaacgg ctatggcaat cacaacaaat
     3181 aatggacaaa gaagaattaa ccctataagc gacgctaaaa aatcaaaact acgtttgagc
     3241 acaatgcaac acctttttga tagcatcaat gacgcgttgt tgatccgcat cgctcatatt
     3301 agtgtataat ggtatcgaaa cttcttgact atacgactct tgtgcatggg gatacatttc
     3361 agaattaagg cgatatgtat ctcgccaata gggttgtaaa tgaaggggta taaaatgcac
     3421 cgaacagcca atacctaact tagacatttc tacaataaaa tcttcacgag aaatttttgc
     3481 ctctttggat aagcgaattg gatacaaatg ccacgcatga gttttatcgt tattacctga
     3541 aaaaggcaaa atcaaagatt catgagccag ctctttttga tatacctgcg ccattttgac
     3601 tcttttttcg tgaaaaccat tgagccgctt tagctggtgt aaaccaatgg ccgctgcaat
     3661 atccggcata ttgtatttga aaccgggggc aataacttcg taaaaccacg ccggtttggt
     3721 ggatgtatat ctatcaaacg catcgcggct aataccgtgc agccgcatca cccgacagcg
     3781 ctttgcaaaa tcttcatttc gagtgaccac cataccgccc tcaccggtag tcatagtttt
     3841 gtttgcataa aaactaaata cggtaaaatc cgaatcaaga gtaccaatca gtttaccttt
     3901 gtatttagag ggcaatgcat gggctgcgtc ttcgacaaca taaagtttgt actctttggc
     3961 tacttgcaaa atttcatcca tttcacaggg taagcctgcg aagtgaaccg gtacaaccgc
     4021 ctttgtcttt gatgtaacgg ctgcctttaa gtcaggtgct gttatattaa atgtaacagg
     4081 gtctacatcc acaaagacag gagtagcccc caaatagcgc accacctctg ccgtggcggt
     4141 aaaagtataa acaggcacaa cgacctcatc gccagggcca atgccaagtg cttcaagcgc
     4201 taagtgcaaa ccggacgttg ccgaattaac cgcaatcgat tgcaaccggt tatcacccaa
     4261 aaattcggta aaattttctt caaatttttt agctttgggg ccagtggtaa cccaacccga
     4321 cttaagagaa tcaatcactt cgtgaatttc ttcatcgccg atttcaggta aggcaaaggg
     4381 taagaagggc tgggtcataa ttctccgtcc atagccgcca agaatcgttt tgccaaaacg
     4441 ggataggtct gatttttcaa aataaaactc tttccttttg tccccattgt ctttcgttgc
     4501 gttggcgaaa gtttagatag ctttactatg gcatcggcaa ccaattgtgc atcaccaggt
     4561 ggcaccgtta taccacaacc tgcttcacct acgggatcgt taccagcttt gatagcgcac
     4621 actatcggtt tttcagacac catataatcc attaacttgt taggtgaaat accatactgg
     4681 aataatgctt ctggttttaa accaatgtag gcaatatcaa tcaattgcag caaattaggt
     4741 acttcttttt tgggtattga gggtaatata attacattag aaatttcttc atctttgaca
     4801 cgttttatta atttttcttt ttccggacct gtaccaaccg ataacaattg cactctacct
     4861 cttaaaattt tcattgcatc taaaagggtg tcgagcgcat ttgccaaacc gtgggtgcca
     4921 acatagccaa ctatcggttg tttctttttt ttaagatttt ttattatttg caaaacctca
     4981 ttattgagac tttctggttt tttccattca tcaagattaa taccatttgg cacatataca
     5041 aattttttag gagctaatcc tctcgattgc atatactcga gcgtcttagg caacatggat
     5101 acaaccttat cggcatggcg atatgcgtag tcttctgcca tctgtaccca aataatgaat
     5161 ggatgccaac gcggtagatt accaagttgt atcggtgata gcggccataa atcatgcact
     5221 tcataaacta gtttggcctt actcaatttt gcaatacgtc ttgctggcca aatatccatc
     5281 ggataggtac ttgaagcaat gactaaatca ggtttaaact cttgtgctat tttcttagca
     5341 tcgcgtttta aatgacgtaa gaataccaac atactcaaaa cgcgttttat tccattacct
     5401 gtataaactg ttgtcttata ccatctatat gaaattccat caataatttc atctttatgc
     5461 ttcccactcg acatctctgg ctgtttactg cgtatatgtg aataggagcc tgctaagatt
     5521 ttaacctcat gccccattcg ctgccactca cgacccatat aataaggacg aaattccata
     5581 ccgtattgtg gcgaacctgc ataatgatta atcagcaaaa tattcattaa tccaaatcct
     5641 tccccaacct tgccacaatt ttttctgcgg catcgcctgc tccataaggc tctacgcttt
     5701 ttccttggct gcctaatgcg ctcataaaaa catcgtgtac agtgtgcgaa tctgttggtg
     5761 gggcaaggcg gttccagccc gcatcgacaa gctccaccca ctccgtttca tcgcgcaggg
     5821 ttacgcaagg cactttgcac ttttcttgcg ccctgatttc acttcaatcg catagacttt
     5881 attggcatgc gtatacataa aatcaacttc gtcgttgcgt tctcgccaat aaaataactt
     5941 tccgggctac tgcatcaact cagctcccac gacaagttca aacgcctgcc aaaatagtac
     6001 agggttggta acccgcctac tttgtatctc tatatgagat tttacaaaat aataggctga
     6061 cctactattt ttaataagtg aagcttgtaa aatgtattta gagaatatca gttatgctta
     6121 attaggagcc tatgcgccaa ggtagttcaa accaagtgcc gcacaacctc cgctgccgca
     6181 cgcccaccgc cataaagctg cgcttcgtcc acgatagcct gtccgttctt tcgctttacc
     6241 gcatcgacaa tcttctgtgt atctgcacct acaagctcgt ttgcacccac ctcaaccaac
     6301 tcaatccact cggtttgatc gcgcatggtg acgcatggct taccaaaaaa atacgcttct
     6361 ttttgaacgc cgccgctatc cgttaaaaca agaccacaat gttgtaatag ccatatcatc
     6421 tcaaaataac cgatggggtt tataacgttc gcggttaaag ttaaaccctt ttcttgaata
     6481 attttcctag tgcggggatg caacggcaac accaccggcg ctatttctgt atgaatttta
     6541 ttcagagcag caacaataga ggccaatcgt tcaggattat cggtattttc agcgcggtgc
     6601 aatgtcgcta aaataaattt atcggcaagg ttaaatttct ggggtgggat tgcttgacgt
     6661 gcaaaaaata acgcagcatc ttgcatgaca tcccccactt ttaatacttg aaccgctttt
     6721 ttagcaaaac cttcggcact taaattagcc accgccgcat cggttgggca aaataatata
     6781 tcgctcattt gatcggtcaa aatacgatta atctcttcgg gcatttgcat attaaaactg
     6841 cgaaggccag cctcaacatg cgcgatgggt atatgcaact ttgccgcagc cagtgcgccc
     6901 gccaaagtag aattggtgtc gccatacaca agtacggcat ccggcttctc ggacaggatg
     6961 atgctctcga tgccagccat catctggccg gtctgctgtc nnnnnnnnnn ttcacgaagc
     7021 aatacttttt cgatttcaat aagcattctg cctgtcattt caccgtgatt gccgccatga
     7081 atgtcaagct ggtagtgcgg gcgcggaata cccaattggt taaaaaaaac atctgacata
     7141 ttggcatcaa aatgctggcc agtgtgtaat aaaatttcag ttaacctgtc tgtttgttgg
     7201 atggctcttg agacaacgct cgccttaata aattgtgggc gtgcgccaat gatggtaagt
     7261 atttttttca tattactata ttatccccac aacgacaaaa atggcacaca ccaaatgtta
     7321 ttttacctga ttgcctaggc caagccagta aaatcaccgg agtatcaagc atagcctcgg
     7381 caatacgcaa atcgataaga cgcctatata ttttttcatc gggcatgtag cctattatcg
     7441 acttttagcc attacttctg tcgggaactt cggaaaacag gggcgtgaat tttggaattt
     7501 ggagtcgggg atttcagatt ttatttacgt aaatcttgga atatagagtc gtaaaactcg
     7561 gaatctaaat tcgtgaaagg gatttctata tgtccgaaac tgtcttgtac aaatccagaa
     7621 actccttctc ttcaatatcc caattataat ttgtatttgt taatctaaaa ataaacttat
     7681 tatttagcct tattgtaagc aagtttgtaa ctgctaacaa caccactata aacctttacc
     7741 atctgctcaa cacagcgtgg ccaattataa gttttttcta catgccccct gccagctagc
     7801 cccactcttt ggcgtaattt agggtttagc actagcttct caagagcaac tgctgctgcc
     7861 tgtgagtctt cacgaggaac tataaaacct gttttattgg ccaacaccac ttctggcaag
     7921 ccaccaacat ctgaaacgac cactggccta gctgttgcac ttgcttcaat aacagcaaca
     7981 ccgaaactct cactatccaa acgactcaaa gcagcgaaaa tatcaaattt cgctaactct
     8041 tgtggcactt gtgagtgcgg cacacggcca acaaactcta ctctgttaga gatttttaac
     8101 ctttcgactt gttgtttata ctgttttatt tctggcccat caccaaccaa acgtaaggtt
     8161 aatctatccg caatattttt atcgctcttt tggaatcttt taaacaatat agcaaatgct
     8221 tcaatcaata tatcaatacc atatttgctc gccattgtct ttactgttcc aacaacaatt
     8281 tgcttattgt tgtgtgcaaa tgctggtttt acattgcgat aaatttgtat gtcgacacca
     8341 aatggtgtta ttgcaatatc ttttaatttt ggtgctataa ttcgggtctg ttttgccata
     8401 cacacacttg ttgaagctac cgcattagcg gcatataaat tagttttaac caaccatttg
     8461 tgcaaagggg atgtatatgg aaaatcataa acatcactgc cccaaaccga aagcaaccac
     8521 ggttgataat taacaacccg agccgtagtg ccatagccgc tggcataatg agcatttaca
     8581 atatcaggtt taatttgttt taagattttt tttactcttg gaaccatagt aaaataccct
     8641 aggataccac gatagggtat tttatgtaag gtaatccctt gcattaatga ttcagtaagc
     8701 gggtgctgtg atataatatg aatttcatga cccgctttat tcagccactg tgcccactgc
     8761 acagtgtgta ttgatgatgc agacgacaga agtgcgattt tcattgagtc aaatcttgaa
     8821 tgacttgctt ggctctagct cgccacgaat gttcagtcga tattttctca agattatcga
     8881 cttttctacg tattctgtcc gggtgttttt ctaaggcatt caacaaacca ataagcgcat
     8941 tttcatcata atttattgcc caaccaatac ggtagttatc tacaaactta ccagccagag
     9001 tctttttact tgccaaaata ggcttcttaa aaccaatata ttcatacaat tttactggtg
     9061 cggcaaactc ccggtattct tgtggtttca caaaaagcac cgcaatatct gctgctaaca
     9121 gttctcgctc catttcatga ccgactttat gaataatttt tatatttgca gacggctcag
     9181 ggtattccgc cctcattttt agccattcat cttcgcgtgt gcacaaagta agctctactg
     9241 ttggtttttg ttttaaaaca ttaaataaaa cgtgcatctg gtaatgagat gaaaaaccac
     9301 ccacataaaa caatttaagc ttggtcgaca caggtttgaa ttgtgttact ggcgaagcat
     9361 gccctggagg cagtgctcta aattttttct gatttatcgt tggtatatat ttcgccattt
     9421 ctaaagatgg cagataaaat ttatatactg ttctttgata cacatataaa tcgaagcggt
     9481 atgccaattt tgcaaccaac acttttacca tatttaaagc ttttctatat tcttcaaaac
     9541 gccaataaat atctcgataa aatagaccta ttaaaatttt atttttttta cagaactgaa
     9601 aaaatctaca atctataaag ggatgagtcg gcagatggtg cttttcggtt aaaatgttag
     9661 gcatcgttga actttctgca tagacaaatt catactttac tccatttcta atgttttttt
     9721 taatctgtct aattgcagtt ttacgctctt tggcgtaacc agtgattaac tcaacttcgt
     9781 agcctaaatc tttaaatgct tgcaacatac gctgagggcg aatgccacta gccgacttag
     9841 cattaggatc aagtggtaat gggtgatgaa agagaatttt tttcattttc ttgaagcgta
     9901 tcagaaataa ttaacaaaag taatattata gatactgctt gagaccaaaa aatactgaat
     9961 tagtccgcat attaaaggac aaagaaatca ttataagaac gagaaaaact taattcgaca
    10021 ttggcatcag gaccagtaag tatcaacctt gaatcagttt tcgcaacaac accatggcca
    10081 tttcatgcaa agagaacggg tattgaagcc taaatcggag acctactctc aaaaattcag
    10141 cacataccaa cgcccaaaat ttatccaacg ccatacctgc caagaaaagg cttttttgcc
    10201 cgcaacaacc gcatcaaatt cttgtagaat tttttcttgc tttagaaaag gcaaatctaa
    10261 ctcggtttta agccattctc taacggtagg tgccatactc aacaaccaat ttttctctgg
    10321 tgtttcaaag ccaattttat cttttcgatc taaaatagta tcaggaacaa taccacgcat
    10381 agcagcgcga aacacatgtt tagtttcgcc ctgtggcgaa acaagatatt tttctggcat
    10441 cgaaagcaat aagtttacca tatctaaggt tagaaatgga acacggcttt cgatactaaa
    10501 acgcatggca ttacgatccc catgacgcaa aagtgaaaca agcccccggt gcgatagtga
    10561 caatgccaat tcatcgataa cccgcctacc cgctatatta ttttcagggc gaatgcgtgc
    10621 cttgaccagt tttacgccat gctcttgtaa gatactccca tttagccatg acggctccgc
    10681 tgacttacca gtcatataac gtaataacga atggaggcta ccttgagtca tttcagatac
    10741 aacaaatttc aaagcttgta tacgactgcg tcctggccat cttgcccact ctttatgaaa
    10801 tcgccacgca tctattaaat ggcctgtttc taaaagacta cgtagacgtg gccccggata
    10861 gccattgtaa ccagcaagca tttcatcagc gccttggcca tctaaagtaa cggtaatacc
    10921 attggctttt gcatgctgaa agacacggta ttgtgcataa atactggtac taccaaaagg
    10981 ttcaccctgt gtacgaatca tatcatctaa atcgtgagct aattcctgtg atgtaactcg
    11041 cactttatgc acagcggcac ccacatgagt atttacgcaa tcgacccaag actcttcatt
    11101 aacgcggctg ccttcagcga taaaactaaa agtatgtatt ggcattttag gttcaacata
    11161 ccgcatagcg caaacaaccg ctgaagaatc tataccgcca gataaggctg cacccaaagg
    11221 tacatcacta cgcagatgca agcgaatatt ttgcaaaaac tgttcacgca cctgctcaac
    11281 agcatcgtca aagttccaat ccccacgttc ggtgatatta ggctgccacc accgcttagg
    11341 tatatctgtc ttacctgtaa gcgtattcac ttgcaaataa tgcccaggca taaggtgacg
    11401 gagctctgca taaaaagtct caggagtgct atcgtaatcg ccatgcacga gatagtcata
    11461 agctcgctgc caatttagtt gtggtttaga aggtagcagc tctaacagcg ctggtaattc
    11521 tgaggcaaaa gaaaaatcgg agctatttgt tgcgtaaaaa aatggcttaa taccaaaagc
    11581 atcacgcaca caagtaagtg tgttcgcatt tttatctaat atcgcaaaag caaacatgcc
    11641 cgctaaacga gataaacaat taacgcccca ttctcgccaa gcggccaata aaacttcggt
    11701 atcagtatcg gtaataaaat gatgccctaa tgctttaagc tcaatgcgaa gttcacgata
    11761 attataaatc tcaccattaa aagttattgc aagggtgcca tcaccagaat gcatgggttg
    11821 atggcctgca ttacttaaat caataatcga taatcgtgta tgccccaata ctacggctgc
    11881 cttatgcaag gggtacacct caaagccgct atcgtcagga ccacgatgtt tcatcttttg
    11941 caaaaccgcg ggtaaccgtt gtattgcttg ttcggggctt tgccaccagc cgcctgttat
    12001 gccacacata tgattaattt tttagtcttg ataataattt ttttagatat tgaggtttgt
    12061 cattcatctg taaagcagat ataccaaatt cattgataat ttttttgata tttgaaaaca
    12121 atcgtttctt agtaatcaca caaccaaaaa tatagtcata cgcattcgga ttataccacc
    12181 acttccctgg aattctattt tcaaagataa gttcaaaacg tttagcaacg actaaaggag
    12241 ttatattttg atatgcaaat ttttgtaatt tttttccttg tttaaaaata aaatgcctac
    12301 ccttcttgag aatctttaac aattcctctt ctatactttc aggataaacg tagcaaacag
    12361 gtggtcttgc ttttgcatca atttgtccca aaacaaattt ttgatcataa atacccatga
    12421 ctaccgctct acccaaaaga gcactttctg aagcaaggcc tggtaaagga atgtcactgt
    12481 aaagctgatc aattaccata tctgatgaaa gaatcgtctc tataactttt tgatttgatt
    12541 gattaataat ctcggtgtat ctaattctat acccttgccg ctttaaattt tgaatagctt
    12601 tttgaatctc tagtgtccct ttaagacttg gtgctgaagg tgcatgcaca attgaaaatg
    12661 gagctttatt atctgttcgc tgtagtatgt catctgcctt ataaatcaca ctcgctgggc
    12721 acataatata ggaacggtta atcacggatt ttgtaaaata agctgcatat gctggatcac
    12781 atacaatata gtttgcaagc ttttctaagc gagtaattct tttttttctc tctttgttta
    12841 gagaaacaaa agtttttata tctacacctt gatatgtgcc atccatataa ggtggccgaa
    12901 catcactacc atgcatcaca aaaataattt tacgccctaa taattttaaa agataaagct
    12961 ccgaaaattc aaaacaagaa aagatctttg gcagtggtga aaaaaatgaa gtacaatatc
    13021 caaaaataaa aatatcaaac ttgaaaatac aactaaaaaa aaatagagct tgaaaaatta
    13081 aacgagaagg gaagagaagt aattttaaga gttcaaagaa gtgattttta tgagaaagcc
    13141 gattatgtag ttgaattaac attcgtataa tattgcataa aagggaacgt gatggttggc
    13201 tatatttaaa tggatgagaa aagtaaagat aatgattgca ttgataacct aattcacata
    13261 aacccttttg aagacctgca tagtaacctg caatttctgt tacacctaag aagattttat
    13321 tcgaataatg cataatagct ttttgattta ttattttttt acaactgggt ttatatccat
    13381 tagctgattt atataagcga atttctcacc aattacttcg agtgaatgat gtttctcaac
    13441 aaattttctt ccattaattc caagattttt caagggtatc ttaccagtat aaatatcgca
    13501 aatgattgat tttacattta atgcagaggc attaattatt ggaacctcat gaggagtaat
    13561 taaaccagca acagtataaa actctaataa ttcttctgac aaataacaga caacaggttt
    13621 accatgcgac attgcctcta ttgaaaacat ggcataccac ccgattatca attgatctaa
    13681 aactacatca cagtcttgta ttgattcttg aatttctgaa tttggtacgc cctgttttaa
    13741 attcaactct attgcgcatc cttcttctct aagctctttg actgcattga tgaaaaaatt
    13801 actgccttta attgctttat gatttggtgc atgaaacaag cgaagcggtc gttttgaatt
    13861 gaatttttta ggtatgcttt catttgagtt aaagtggatt ttcgctaaat caatacaaaa
    13921 atgacctaac attaaagtat gccaatgcgg tacataatac acccaatcgc accccgaact
    13981 tatatgagtg ccatactttg accagcgtgc cacttgcttt tctgttttaa cttgctgaag
    14041 ataaaacatt ggataatcat tatctaaagc gcgtttgaaa ttaaggttca caactctgcg
    14101 taaatcatgc acatctccac cgtaagctgt aacgtgaatt ttgatttttg caaatcgata
    14161 aatcagtggt tcccacttcg ccaaaagcgt ccatgcaaat ggaccaccat tataatagat
    14221 ataaaggcac tcataccgcc atagcaccat aaacccaagt aagtaattag agagaatatt
    14281 tattggaaaa ggcaatttat ccacacggat atcaaattca tccgtaataa aatatacttg
    14341 atttacaaat gtctcagcag taaatccaaa ttcttcaaga gcacgtttat ggtataaatt
    14401 attaatgaga ggttcaggac caaacccaat ctgaataggc tttcttttat atcgtgatat
    14461 aataatcaaa attataagaa atggcaatac cacaaccaat ataacaccct ttattacgaa
    14521 ctggaaaaga gagaaatagt aaatccgact aagaatcttt ttcatttttt ttattccgat
    14581 atttcaaaac tgcaagcttc ttggtatctc agtttttgct aaaaaaactc gacaccatcc
    14641 ttcaaagaat gcaaggctcc aaattaacat tctttcatca ctgcctaaag catgtctatt
    14701 caatattttt tcaattactt ttggttcaat atattcatga catactgtct tatgtcccaa
    14761 caacaaattt cttaaatagt cacttaactc tctacgatac catgtttgat ctggtggagt
    14821 aaagccttgt tttcttttat gaattatttc ttttggcaat aatttatcaa aagcacgctt
    14881 caaaatatac tttgacacac cattacgata tttatacgca tcgggaataa gaacattaat
    14941 attaatcaat tcattatcaa gaaacggaac ccgagtttca actgaaaagg ccatccccat
    15001 tttatcttca accatcaaca taccgtgcaa aaacgttttg ttttcaaaat aaagagcctt
    15061 attcaaaaat gaaacatttc ctgcattgtc aataatgtgg ttaaatacct gccgtgagcg
    15121 ggtaaaatct atttgattag caaattgagg tttaaagagt gttattcgtt ctttgtcatt
    15181 aagcaatcga caccagtaat tataataaat attacgaaac tcactttctg attctacatt
    15241 ctgaaaaagg tggtatcgcc atggataacc tgcaaatatt tcatctcctc cagttcctga
    15301 taaatttact ttaacgcgct gtgagacttt ttgagctatt gtatagaaag tataacacat
    15361 tgctacttta ggatcctcta aataccaaac aatttgtggc aaattatcaa tcatatcctg
    15421 aggcgatatt aaacgctcat gatgcgttgt accaaacata tttgaaatta gtcgtgcatc
    15481 atctttttca cttacagccc tatcattagt ttcaattgca cttgtatcaa aatatccagt
    15541 aaatgtgtgc atccctttta ttttttggct agctaatgca ataattgcag atgagtccat
    15601 accacctgat attgtagcac caatatcgac atcactaagc aaatgacgat ctagtgactt
    15661 atgaaaagct tcatgaataa gttcgacata ttcttgttca tctaattgct gtggtgcata
    15721 cacaagatcc caatatcgct ttatctgcaa atctttttct cgtaattgat attcaaaatt
    15781 atgacctgct tcaagcatac gtacgcctgc aaacaaagtc cgatctgata tgatattttg
    15841 aaaagtaaaa tattcattca ttccttccat atctggcttt gcatgtatta ggcccgtaga
    15901 taaaattgct tttacttctg aagcaattat caaaaactct tttgacaggg cataatacaa
    15961 cggtttaaca ccaatacgat cgcgcgaacc aaagatgacc tggcgttctt tgtcaaaaat
    16021 gacaagcgcc cacatcccgt tgaagcgctc gacgcatttg ttaccccagg catggtaggc
    16081 cttcagggcg acttcggtat ctgaattgga atggaaggtg tagccaagtt tcaacaggtc
    16141 agcgcggata tcccggtaat tgtagatttc gccgttatag catatgacgt ttccggattc
    16201 cgggtgcacc atgggctggt gacctgccgt agatagatcg agaatcgaaa gccggcggtg
    16261 acccaaatga acacccaggg cgtcttctga gtagatgcca taatcatcgg gaccccgatg
    16321 gtacagctgg tcggtcatat tgacgacgcg gtggcgcgcg ccatcaacct gtccgaatag
    16381 gccggctatt ccacacatat aatctcttac tgtaccactt tcgagaggta atctctaaat
    16441 tcgtcgagtg ttcggttcag tgttccccaa aagtcttttt caaagctgac attcaggtca
    16501 gtgcagacag catgattcga gacgtcaaaa aacatgatgt cgcccagcag tggttcaccg
    16561 tgaatgatct ccacaccgga ctgcatcatt tctttcatgc gcagggccag gtcattgatt
    16621 gtcacctgta ttcccgaagc ggcattatag acagcgccgc gagctttggg attcatcgcc
    16681 gccgcaaggt taactttcgc gaggtctttt acccatgtaa agctacgcat ctggtcgcca
    16741 tggccaaaga tggtcatggg cttgttttcc ataatattgc gcaggaagat agagaccaca
    16801 ccgccaaact cgttcgattc ctgccgtggt ccatagacgt gaaaatagcg cagaatggtg
    16861 gtgtcgaggc catataggtg atgaaagaca tcgacatacc tttcgcctgc cagctttgac
    16921 acgccgtagt acgaaacggg ccgcagagga tgatcttctt tagtcgggaa tattgtcggt
    16981 tcaccataaa cggaaccggt tgaggcatgt acgaacttac gaactttgta ctccnnnnnn
    17041 nnnnagcaaa ttcagcgtcc cgcccgcatt gacgtcgagg tcacgctgcg ggttgatgag
    17101 acagatattc ttttttgaag cggcattgtg gaaaatcgtg tcgcagtcgg tgaggatttc
    17161 atcccaacca tctttgtcgc agacgtcttt cttgtaggcc ttaaatttcg ggttcgcctt
    17221 catgaactcg atgttccgct ctttgccagc cgaataatca tctatgctga tgacctcggc
    17281 accggcatta agtagttctt cgcagatatg ggagccgatg aaaccggcac ctccggttac
    17341 tatagctttt ctgcctttta aatccatttt tgatttctcc taggtttttg gtaatattct
    17401 aaagttcagt taatcttctg gatacgccag ggtaaatccg gatgtatgac tccgcctatc
    17461 cgagcaccaa ctgaatagcg ttttgggttg tccagtttct tgtcgtctgt tataagaagg
    17521 ttgatcacat tttcgagctt tacttcattc ccagatgcat gtttggtgta tgaattgata
    17581 tagaggttta taccatattc atcctcattt agcagattgt ccgggattct gcattgcgcc
    17641 gtgtatcgcc cttttgtcaa cggagcggac gaaaacggcc atgttatgaa taccaagatt
    17701 cccagactat tgatcacatg caaattcgga attataactt catcagtatc ctggagaatt
    17761 tcataatcaa tctcgatgag aacgctatca gtgagtgtat agcgttgctg ttcggcctgc
    17821 gtttccccat gcctgcaaat ggcagaaata aactgtgctt tttgtgattc ttttaggcct
    17881 aatgcatgtg cagcttccct ggttatccgc gcacttgcaa gggacctgtt ataagtctga
    17941 atgacgctag tgatttcgga atcagaagaa actctgccgt tctccagcag tattccgcgt
    18001 tggcatagat tggtcaaggt tgccatctga tggctggcga aaagaaccgt cctaccttct
    18061 ctccgtaatt cgcccatctt ccccaagcac ttcttctgaa attgcacatc gcccactgcc
    18121 agtacttcat cgacaatcaa tatttcgggt tccaaatgcg cagcaacggc aaaagccaga
    18181 cgcacataca tgccgctgga atagcgcttt accggtgtgt cgatgaactg ctcgatttct
    18241 gcaaaatcga cgatttcatc gaacttgcgc ccgatttcac gccgcgtcat gcccaaaata
    18301 gcgccgttga gatacacatt ctcgcgtccg gtaagctcag gatgaaaccc cgtaccgacc
    18361 tcgagcaggc tggcgatacg gccccgaatt ttcaccgaac ctttcgttgg ggcggtgatg
    18421 cgcgacaaga tcttgagtag ggtggatttt cctgccccgt tgcggcccaa gatacccaat
    18481 acctcgcctt gttgtacctc gaaagagaca tcttttaacg cccagaaaaa atcgtcgtta
    18541 gcccggtgtt gagagctctc accgatgcgg cggtttgggt cttctttacc acgtgtacgc
    18601 gcccaaaaac tctgtaagtc tttgtgtagc gacccagtgg atatactacc caaacgatat
    18661 gactttgata gattttcaac tttgattact gtcttcatac caatgttaaa tagtgtcaat
    18721 aaagctcttt tctactcgag caaacataaa aagaccaagc aataaaataa caagagttat
    18781 cacaaggctg ataaccaagt cgttaccaat tgctgggaca agagaggtac tcgcagaacc
    18841 gccaaaaagt ataaatcgta aatttgtgac aacctgtgct gcagggttta ggtttactaa
    18901 aattcgatat ttttcaggta tttgtgaaat cggataaata attggcgtgg catacatcca
    18961 tagttgaaca ccgaaaccaa ccagataatt aagatcacgg tattttgtag tcagtgatga
    19021 aatcagtatg ccagtaccta aacctaaaaa cgccatttgt ataataagca tcggcaatac
    19081 caagggtagc caaggtgatg gttgaactgc ttccccttta aagagaaaat aaagataaat
    19141 cagagcaagt aaaacaagct gaatcgctaa tttgattagt ttggaaaaaa taattgatat
    19201 gggtatcgat agacgaggaa aatacacctt accgaatata ttctgattat cgagaaaagt
    19261 gcttgaagtt gaaactagac aatcggaaaa atagttccac agcaccaacc ctgaaagata
    19321 aaatagaaca gcaggtactc cgtctgttga tattgatgca atccgactaa aaattactac
    19381 aaaaatcata gtcgtgaaaa gtggctgaat aagataccac aagggcccca aaatagtctg
    19441 tttataaaat gctacaaaat cacgtcgcac aaacatagat attagatcac ggtatctcca
    19501 cagttctcgc caatcaatac tgaaccacga tttttttgat cgaataacta tatcccattt
    19561 ttcttctgaa ctagaactac tcacttgtcg acgcctactt tattattttt tagatatttc
    19621 acctttatac tactttttga atatattggt taccctctat gcgttcaatg ccatttctcg
    19681 aaattttacg ttcttatcga ccaactcacg atacgtgccc gaatcgacca ctctaccacc
    19741 ctcgagtata taaataatgt ctgagttcat cacggtcttt aggcgatggg caatcaaaat
    19801 aatggtgcgc tcaccgctga gttcatcaat cgcattcatg ataatttttt ctgtgatgcc
    19861 atcaagcgcg ctggtggctt catcgaacac cagcaccttg gtgtcgttgt aaagcgcacg
    19921 cgcaataccg atgcgttgcc gctgcccgcc cgacaaacgc acacccctct cgcccacttt
    19981 ggtttgaatg ccttcgggca gcgtggcgat aaagtccgcc aagtgcgcaa gctgcaacgc
    20041 acgattcacc cgtgccgcat cgatttcact gacacgcaaa ccaaacgcca cgttctcagc
    20101 aatagtacct tcggttaaaa aaatactctg cggcacaaaa ccaatttgcc tttgccatgc
    20161 ctcgcggttc tctacggtaa taggtacacc gcccaccagc aggctgccct tttgcggtag
    20221 aattaacgcc atcactaaat caatcgctgt tgacttaccc gaacccgtcg cccccaccaa
    20281 acctactgtg ctgttggcgg ggattataat attggcttgg atcagagccg gttcactttt
    20341 tgtgggataa gtaaaactta catctttaag ttggattgat cccattacta aatcaatttt
    20401 tttactgtta agtgtcattt tcccttcgtg aacgagtaaa gctgatttta aatcgtcctt
    20461 aatcgcatga aaggccgcag cgttgccttt catggtaaca acgcttgcat aaatctgctg
    20521 taacgcaggc aagagcttaa aaccagcgag gccataaatg gcaattagcg gcaataccgt
    20581 acttagatca ccattgtaaa agcgtaccaa gactaaaatc aaactaacca ttgtgcaaaa
    20641 cgccaaaaac tccataaagt accgaggtat ttgaccaatg gcacttataa gtgcttgtgt
    20701 acgggtaacc ggcacacctt gattagcaaa ctcttctgta aaagtcacac cacgctgtag
    20761 cattaaaata tctttaatgc caccaaagcc ctcgatcatc aaccgatacc gaatctcaga
    20821 tgcacgtgac agatttttat catagcgcat caatcgattc ttaaccagtt tataaacaat
    20881 gaagtagccg ctactaaaaa ctaaaaggcc taaaatagct acccatgggt taaacacaaa
    20941 aagtgacaca ccaataaaaa cagctaatgc aactcgtgca ttgagttgca gcatcggtaa
    21001 taaaattcca tgcgtaactc gacttgtctc cgattgaacg tggttaatga gttgcgaact
    21061 gctactcgat acatgaaaca accaatccct ttgtaaataa aaacggtaca aacgattact
    21121 catttcagta ccaacagcaa aaccataaaa cgaaagccgc caagtggtga caatcgatac
    21181 caaggccgaa aagcccaaca aaaaaagaac agtgagccct gaaaaaaata aaaaatcttt
    21241 ggggctcgtc aaaccgctga gcgcatacac tttttgcaat gtcgcgttgg tgtttattaa
    21301 attcatatca cccaccaatg ccataaacgg cccaatcgag gcaatgccca ccagttccat
    21361 cgttgccatg acgatgacca acaattgcag tataaaccac tgccgtcttt gagtaggtgt
    21421 caaaagacca aatatttctt ttataagccg aatcaatttt ctacctatat gcgctatgtg
    21481 ctacaacaat aataagcttg aatgcagcct tcaccccacc ctatacttct gcttcggact
    21541 attcggcttc tcagggattg tctgcacaag tagccccttt gccaatagcg gatctaaaat
    21601 ttcatatttg aaatgttggc ggtgctttaa gcctaaaaaa ttcattattt cagttctgct
    21661 tttcaggcct tgggataatc acccgaaatt gttcaccctc tatatcgtta atcagttcta
    21721 tattgggctg tgcttgtaat gcacgggtaa tgccagaacc aaaacctctg taatacatca
    21781 tcttagaact atacgaaact ataaggttat ttctgacgac tgcgtttccc agttttatat
    21841 tttcaatggt taggctgtta ggcaaggctc ctgggcttaa aatttcaatt cgatcatcaa
    21901 aaatcatcac acgtactgga gaattttttg aatagtctct atgcaccaaa gcattctgca
    21961 ataactcttc aagtgcaatt tgcgaaactt ctaatttgcc aatcgaatta aaattctgac
    22021 ctgcctg
//
LOCUS       DAOMPD010000003        18713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1287_length_18713_cov_3.261979, whole genome shotgun sequence.
ACCESSION   DAOMPD010000003 DAOMPD010000000
VERSION     DAOMPD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 18713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18713
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_18713_cov_3.261979"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLY93_00255"
     CDS             complement(<1..413)
                     /locus_tag="PLY93_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRP67953.1"
                     /translation="MMFFYNSLLCFFYPLLWLVSRFHKRLGENFALRAHLPDFAAAKG
                     KTHIWFHASSAGEFEQVRAVAVEFKKNYPQTKFFFSFSFFSDSAYRAKKNDSIPDILF
                     ALPFDFSWRMKRLVRAMRPDALVIGKYDAWANQVL"
     gene            complement(410..700)
                     /locus_tag="PLY93_00260"
     CDS             complement(410..700)
                     /locus_tag="PLY93_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRP67954.1"
                     /translation="MKKGENLHRRYCISGKVQGVGFRQFTQQTAQKLNIAGWVRNLPD
                     GRVETEAEGDAVQLEAFEKALREGPPLGRVDGVGVNESTVKGNLPTPFEVRR"
     gene            complement(744..1775)
                     /locus_tag="PLY93_00265"
     CDS             complement(744..1775)
                     /locus_tag="PLY93_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRP67955.1"
                     /translation="MAFSLSSMLKVFFSKKKRHNPSDPLFTELDQPKKLNRPITRDEF
                     LRVYETMPETARVNLFISLNAAQKERLLHLLAHQNEVKDKSHREEEKQWLAEDFLPVK
                     DEKRVEKLRQVKELKDLKELYSSFEKYTPKVVADILSGEAAHVAAVVLLQFEKRFASQ
                     VVRFLPELSRGEIVRQMASERHIASDALLALGKKIATLLTEHPQQVGETIDGIKHVND
                     ILKLMGVDEAERITREISHVDDTLASHLEKSRYVFDDLVELNSRDFRTVFSAIPDTEL
                     WARALKAFDQSKRKSLLGKLPIKRAAAIVEAMAQVKTTRLDSIDKARGKILREALRLA
                     AVHEIQLGR"
     gene            complement(1779..2105)
                     /locus_tag="PLY93_00270"
     CDS             complement(1779..2105)
                     /locus_tag="PLY93_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP67956.1"
                     /translation="MLRTKEVGSYIIAYLKGRLDVHLSGEIEKDLMALSEKNPEADFI
                     LNLEGIEYMSSSGLRVLVALMRHLKTKKRQLKLCNMNQPVRKIFEVVELMDMFDIYDT
                     EEAATK"
     gene            complement(2112..2537)
                     /locus_tag="PLY93_00275"
     CDS             complement(2112..2537)
                     /locus_tag="PLY93_00275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP67957.1"
                     /translation="MPRLKKNFTAERAAIPIVHDFVKSALKDHGVSVETATDFILACD
                     ETATNIVEHSFVDKINVKEAKPQFILTLCCRKNKVTATFFDSGASFNLKDVEAPDISK
                     NLRGEKRGGYGVFLIRKLVDKIVYSARRRLNVTRLIKET"
     gene            complement(2551..4920)
                     /locus_tag="PLY93_00280"
     CDS             complement(2551..4920)
                     /locus_tag="PLY93_00280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP67958.1"
                     /translation="MSSEELTTYSSSFTHLLQQKDDRVFVEATAELNKATLYILKAAN
                     SLMHSKHCYLALRTAQGVKTYRTAMQQLTVLEKKIMQKSLVEGETFAIEKRQRLFNSV
                     QVSMGEAETTYLAAPLITDDKAALGSLLWVTKQGEELFSPEDLSLASTFARTFSRMLA
                     SCIRSPDNTALLVKFSSNLVTIFENLYLYRHNVENNFLLSEMIKVSKMINSTLDLNSL
                     LESIMDSAKIVLKAEASSLMLIDRKTNELFFNVISGEKERDLKEIRIPLGVGIAGIVA
                     ENCKPLIVNDAQNDPRVFKEADAKINFVTENMIAVPLMVRNRIIGVIEVINSIGRDEF
                     SEKDLELFNTFSEQAALASHNRELIDSLKDINRELKKKVHELSSLHEISKVLMSTLNE
                     KDLFDSIVRIIADEMQADRVSIMIYSAEYDALEVVSHHGLNLAPFDRSFVPLEKSLAG
                     KAYETSKVIYTNALEKTPYSAFRNIERYHTGGCIIQPLMHGRDIYGVLNISDRQSTLT
                     HEFNDDDLRLVATIASQITRSIQNFRLLEEMLQKRSYEKELEITSSIQKSILPTRFQK
                     SSWFDMGVVSESAKLMGGDFYDFLSFDGDQVMFSVADVSGKSLPAALFMAVTSSILRT
                     ISREIHAPAEVLLKANDLIYQDSQSGMFVTLFYTFYDAPEHRLYFSSAGHNEQLIFRH
                     DTHTFEKLGTKGRPLGVIDSATHGPYGEGSAFLNVGDILVLYTDGVVEALNDREEEFG
                     MERFCRVINDNMLLSSDQLAKKIFREVKTFAGNVAQYDDFTLMVVKVLK"
     gene            complement(4923..12560)
                     /locus_tag="PLY93_00285"
     CDS             complement(4923..12560)
                     /locus_tag="PLY93_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67959.1"
                     /translation="MRRLALLLFAAFLAAHCASGNKRDDSRFSYENLYQDRYQRGDGG
                     IIPVTIDRGESYSGSISRDQKYLYFSSNTAGNYDLYLRDLADVFSIPVVSKVTNQREP
                     TISPNGKYLAYVDDELDPDGDIMLLKVNPKKLIQNYQDKDTSYDERFTPRAENLTNSE
                     KHRIRARDANPAWSPDGDFLAWSTDLNLKKADDLGAGAGALQNIWIMPASSPEKKRQL
                     TTEGGVMPSFSPDSKRIVYISYQDQNSNGAVYELEIATGKTRRLTSGKALDLYPSYTP
                     DMKAVVLTRIARDTNGDGSLDRKDAGQIIRIQPKNFESTKPFVDDESVFVEDNERVAL
                     TAENDSVFDSRVSNFIGGSIILAQVKGEDINIGFIPLSGAVPVKGDILQQEEYLTTLI
                     RKTKNKNLPCLGFEQLPSAFTNSPDLVVYRELSFMRESACNKSAAQRLQHVLNQSGEK
                     EKVLYRLFNDLSIISPDYADLKGVLPLPPLADTRNHAEYFSRILVKKDLWNNYNNEPI
                     EFEKQTPVISPEAIDSKSEIKSEVESIKPKQVDPRFIAVQSFIRYEQAKVLIREGKHR
                     EAVTVIRKILQLNPTYIGLDTLFLENGILDSTSTPAPEIVFLLAEKPDAKLLPAYFSK
                     LPIQTIPIRVAVRRKAERFLSTFYRTQFAGGNIVVQEQYLVDLPTKNYRMLHAMFALQ
                     KARAKIKEGEYDEASTEAKRTLELSTKGTINYFDGQLQLARIAEVKEGAAAAFKIHES
                     AIANFRDTEIPQYAKLIISKNRIYHTERAAQFSRERNDRAAATEYMALLDLFLSTHAA
                     KLTSELNSGEILETALNLDDIALRAAREEGGDEKLAKDIIQFYDSRIDLARRYLVTEF
                     IFGRGYFRAQLGINKHSAAEREGLTKSVKKEVFVHFLKAEIDFNWSFFANARFADAYI
                     MLGWMYQFIDEKREVVLDASSGKKDREIFESLYKAYFPDYLFEKNIRLYQKTLTIFAN
                     AASKRVKNSFHLNIANNYFLLNNYSQAEEHYAAILDKKGNPDFRFETPEQEMMFYYHF
                     GRTLYFSGKNEVAMRYLQFVEDNLNSRYPIAGVSAEIQKTNAARRAIAYKTFALNSEY
                     AGQVSRAIYYHRTILSEAKSISSDTPRSLSHLELARLYLKQDNYGAALWQTNNAEKEL
                     KKEKEISIPKFKIRIKWLWVYEPWTTIVGWIYKLSYDDIYIGENHLAFELPTVNRYQL
                     LYSIRADVYKHKGLIQEASDSLAKLVEYAEKDKTKHGKETLAAAVSRRGELEFQLQNW
                     DSAMSLYQTAFKQAEKDKNVHAAQTFRKNILLCKLRKLETGNESIASAIKTAERNAAE
                     VQEYENDFVAARLKAERKRLKELEEGEIRELTDERATKLTLAAQQEIQSILYFKAMNL
                     AHAAILYDFRERFEAREESFDDYLKHKKTTFDRFSSVLNYFRGYTQESTVNIDKVYEP
                     SLKNNALRLKLAMNRAKVLQEMSLVDSAIAEIKEIEERSQEFRADLIYAIAAYRSYRI
                     YEDAGMEENANLGQYKNLFNFFLKHQNFLRQNSDLFERISYIVIDRALKSRNYAEAIR
                     VEDLRRQMLGLQVYFDNLKFDNTKDDHFDKLLVLEQRRYTLAKQIELERFARTNVAPL
                     EKELVAIDNRVEQLRGQLQQADALGYHYNTFFSLGYSDNDLQSLADGGFIYALKPRND
                     IIYIFAKAEGGRKKEITYSTTPVEADDDAIVVLEELLQKTQAKTVVLAPEILTAAMES
                     ISVRLLDNQTTLRSAINFSKNIAEAKRNVLQFVKTSTFLGFSSSNEVRYKTNLTVDYV
                     SKRNDFLDLKIHRNVIDYEGELVKKGVIADTASRTPAELFSVRGKPNYAVASFVARNK
                     MNDADNFRFAAASDLYYSALGARQVLYTFASRKDSQTVIESYLNVDDAPSSALVTGAS
                     IDTSAVEEKSFERQRSYYLKQIREARQKRDYEIATSYAQDMLSFFPDNVDYQLLAAEL
                     NFIGGNNEAAKRYISMANVSEWTPLAARKSYARLLLRAGSLVEARAFIEADDAVANAL
                     RRESAEAEGLYQLSSFESGNFENLTGDFPWGRSVSPSAKTLRKKIPAGLKDSEMLNDI
                     CTAAVASLEYALVANACVSIKNATEQDKENRLQIRRWFNESQINLQIPADADNLDFLQ
                     TLSLMKSGYIGDSLDFAKKYLLRTELSVSENLLIFSLLRVFATRQANTEEIKQLAQIL
                     AQYGKNASKKAKNFQAKTFYELLSLAEDVSKKGVSALDEIAKKKTTPWSSGLQSAYNN
                     LYFAALTVNPNDEILSVWQKTGFVADKKIESDFQFYVSTKENDEFEAINCDNASCALF
                     IKRYLVLDENNKALKLALQSQGKANVAITSLLDGAFGYIEVFHDEVYEWFKDGQKIKF
                     SRLKKFDEKYFTQLSRSRKYLAYTPRRNLFARHKIPVPRKAVLFDVSLSSSKNNVDFQ
                     TLSLAAPEASTLYSALYARWGSSRDKEKAAVIQIKPPFHTAVGALNIYTRNISLDDLP
                     RLKPAAYHLFCLDTESYNSFALFAQSIVNLMVEKGLSVEDAYEVAFTKGAVNKTKIRP
                     FYYLYRN"
     gene            complement(12570..13316)
                     /locus_tag="PLY93_00290"
     CDS             complement(12570..13316)
                     /locus_tag="PLY93_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67960.1"
                     /translation="MKKNKKDIFRKAIADDLGENREDASALGDFVSDMQRKRGDTGDN
                     YASESKNGASAENQKSKNDLPEQKEEKRTGSVMESRLKRLTSGEQSPVQPAAFADDDE
                     PPRWRRYALFAVVGLLALTILYWVIFSTAQKPTIYLANTAIEEANTRHLGTTNLVFAR
                     GKTLYLLFSSGGRLGQDKIIAKLTEVYADASNMIKEESVAQVEGSVKPTWRYFTTQFQ
                     KDNFTHAGRYRVHVLGANGEVIASQEFRVQ"
     gene            complement(13313..14101)
                     /locus_tag="PLY93_00295"
     CDS             complement(13313..14101)
                     /locus_tag="PLY93_00295"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRP67961.1"
                     /translation="MAVVLSITNQKGGVAKTTTALALAYAFARRETEKKILLVDLDSQ
                     RNATSILLGSREITPENSVYALFQGKKNVGAMLHPTKLKNFFIIPSSLHLVEVESQLT
                     GAIDGFFRLNDAIETLRHEFDYIIFDCPPSLSIITINAMVAADFLLVPLQISKFSIDG
                     IQNIRDAVSTVNRRYNAHLKIAGGCLTLHDNRTTLSQAMIPEVEKILPILKTTIPRSV
                     AVEEAHLLRQDIFEYAPENKVAKAYDALAEELKTIFVMPSQHEP"
     gene            complement(14129..14758)
                     /gene="fsa"
                     /locus_tag="PLY93_00300"
     CDS             complement(14129..14758)
                     /gene="fsa"
                     /locus_tag="PLY93_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRP67962.1"
                     /translation="MEFFIDTADVGEIKAAHELGILDGVTTNPSLIAKTGKPFKAVIQ
                     EICSIVDGPVSAEVLAVEKDAILKEGRELAKLADNVVVKVPLIPEGLKAVRVFTDEGI
                     QTNVTLCFSANQALLAAKAGATYISPFVGRLDDISTDGMDLIEDIKTIYDNYEFVTKI
                     LVASIRHPIHLKQAAIIGADVATIPYSVFPQLVKHPLTDAGLEKFLADA"
     gene            14985..15832
                     /locus_tag="PLY93_00305"
                     /pseudo
     CDS             14985..15832
                     /locus_tag="PLY93_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
     gene            complement(15833..16570)
                     /locus_tag="PLY93_00310"
     CDS             complement(15833..16570)
                     /locus_tag="PLY93_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRP67963.1"
                     /translation="MRYFFHIFFLLYLVLPLSAGGNKTILSFAKAKRSAWQLIEKHPY
                     TFYCNCRIEEREIQRGSCGYVPRRDNSRANRIEWEHVVPAENFGRSFIEWREGDERCV
                     RRDGRTFKGRQCARKVNTTFRRMEADLYNLYASIGELNGDRSNYRYGIVSKKVQAYGA
                     CEFYIDEKIVEPRASIRGDIARVYFYMNDAYPNRGILGDKMRRLMEAWDREDPIDEAE
                     CRRATLIEKLQGNENRFVSSVCRGKGW"
     gene            complement(16577..17767)
                     /locus_tag="PLY93_00315"
     CDS             complement(16577..17767)
                     /locus_tag="PLY93_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67964.1"
                     /translation="MNNGSFVNSLDLLTEVEKLAHVLKVSPEKLQFLKNLSIDEFQEL
                     NQAISDRLLTDSSNVWSKLASVAKFMPNFINAQVAKSILGPNITANLTYHIDVKQAVS
                     IAKSLNIDFLAEVTENLVPARAKHIIEAFPMDMLINLTRVLTREKKFFTMGAFVDYMD
                     IDKINALSKEIPDTESLLRVGLYTQNKAHVAKLVDSFSDDKIVDMVETAERRDLWPAV
                     IGLLAYFTDEQHQRLARLVGRVSVDALLGMVPFAIQHGALVYIFAFLKHLSGDMYAHM
                     ATICAKLDSEALARLIEAADESGELSTALKIADHLAERDVERLSAMSHRFEDALLKKV
                     ILTAHAEGLIPFGLRLLALLPETEIERAERIAQSIDRKVIEDAQAQIDQALLKDRLGW
                     LRKL"
     gene            complement(17804..17877)
                     /locus_tag="PLY93_00320"
     tRNA            complement(17804..17877)
                     /locus_tag="PLY93_00320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17841..17843),aa:Val,seq:tac)
     gene            complement(18030..18305)
                     /gene="rpsT"
                     /locus_tag="PLY93_00325"
     CDS             complement(18030..18305)
                     /gene="rpsT"
                     /locus_tag="PLY93_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRP67965.1"
                     /translation="MPNIASAKKRTRQNEKRRRQNQSQKSEIRTQEKKLRKLVAEKKV
                     EEITVAQNKLASLVDKAAKTNLVHKNAAARKKSRIANLVKKATTPAV"
     gene            complement(18373..>18713)
                     /locus_tag="PLY93_00330"
     CDS             complement(18373..>18713)
                     /locus_tag="PLY93_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRP67966.1"
                     /translation="KVDRSAAYMMRYVAKNVVASGIANQCEIQVAYAIGVAKPVSLVV
                     NTFGTGKIGDDQIEKIIAANFDLTPRGIRETLNLRNIKYRPTAAYGHFGRTGQDSFTW
                     ENTDKAAVFK"
BASE COUNT         4925 a         4328 c         4130 g         5330 t
ORIGIN      
        1 tcaagcactt ggtttgccca cgcgtcgtat ttaccaatga caagggcgtc gggtcgcatc
       61 gcacgcacca aacgtttcat ccgccaagag aaatcaaacg gtaaggcaaa aagaatatcg
      121 ggtatggaat catttttttt cgcgcggtat gcgctgtccg agaaaaaact aaatgagaaa
      181 aaaaattttg tttggggata atttttttta aattcaaccg ccaccgcgcg cacttgctcg
      241 aactctcccg ccgaggaagc gtggaaccaa atatgcgtct tccccttcgc tgcagcaaaa
      301 tcaggcaggt gcgcacgcaa tgcaaaattt tcacccaaac gcttgtggaa acgggagacg
      361 agccacagca acggatagaa aaaacaaagt aaactattgt aaaaaaacat catcgacgca
      421 cttcaaaagg ggtaggtaaa ttacctttaa ctgtggattc attcacaccc acaccatcca
      481 cacggcctaa aggcggtcct tcgcggagcg ctttctcaaa tgcctccaac tgcactgcat
      541 ccccctctgc ctctgtttcc acacgaccgt cgggtaggtt gcgcacccaa cctgcgatgt
      601 taagtttttg agcggtctgc tgagtaaact gtcggaaacc aacgccctgt actttgcctg
      661 agatacaata gcggcggtgt agattttctc cttttttcat agttaataag gccgttgtgc
      721 gcgcccacgt caatagggat aaattatctt cccagttgta tttcgtgcac tgcggctaaa
      781 cgcaacgctt cgcgcagaat tttaccgcgc gctttgtcga tggaatctaa ccgtgttgtt
      841 ttaacttgcg ccatcgcttc gacaatcgcc gcagcacgct ttattggcag ttttcctaag
      901 agcgattttc gtttactttg atcgaacgct ttgagcgcgc gcgcccacag ctcggtgtca
      961 gggattgcgc taaacactgt gcgaaaatcg cgtgagttca attcgacaag gtcatcaaaa
     1021 acataacgac ttttttctaa gtgagacgcc aacgtgtcgt cgacgtgcga aatttctcga
     1081 gtgatgcgtt cggcttcgtc gacgcccatg agttttaaga tatcgtttac atgttttatg
     1141 ccgtcgattg tttcgccgac ttgttgtggg tgttcggtta atagtgtggc gatttttttg
     1201 cccagcgcaa ggagcgcatc gctggcgatg tgacgttcac tcgccatttg acgcacaatt
     1261 tctccgcgcg acaactcggg taaaaaccgc accacttgcg aggcgaaacg tttctcaaat
     1321 tgtaaaagaa ccactgccgc aacatgcgcc gcttcgccgc tcaaaatatc ggcgacgact
     1381 ttgggggtat acttttcaaa cgaggaatag agttctttca aatctttgag ttcttttact
     1441 tgccgtaatt tttcgacgcg cttctcgtct ttgacaggca aaaaatcttc cgccaaccat
     1501 tgcttctctt cttcgcggtg ggatttatct ttcacctcgt tttgatgcgc gagtaaatga
     1561 agtaatcgtt ctttttgcgc agcgttcaac gaaatgaaaa gattcacccg tgcggtttcg
     1621 ggcatggttt cgtagacgcg cagaaattcg tcgcgggtga tgggccgatt taatttttta
     1681 ggttgatcga gttcggtgaa gagtggatcc gacggattat gtctcttttt tttggaaaaa
     1741 aaaactttaa gcatcgatga cagtgaaaac gccatgcttc acttggttgc tgcttcttcg
     1801 gtgtcgtaga tgtcgaacat atccatgagt tcgaccactt caaaaatttt acgcaccggt
     1861 tggttcatgt tacacaattt aagctgacgt tttttagttt taaggtgacg catgagcgca
     1921 accaacacgc gtagaccgct tgacgacata tattcgatgc cttcgaggtt taaaataaag
     1981 tcggcttctg gatttttttc actgagcgcc attaaatctt tttcgatttc accacttaaa
     2041 tgaacatcga ggcgtccttt tagataagcg atgatatacg aaccaacttc ttttgttctg
     2101 agcatttttc cctaagtttc tttaattaac ctcgttacat tgaggcgacg gcgtgcactg
     2161 taaacaattt tatcaaccaa ctttcggatt aaaaacacac catacccacc gcgtttttcg
     2221 ccacgcaaat tttttgagat gtctggcgcc tcaacgtctt tgagattaaa cgacgcaccc
     2281 gaatcaaaaa atgttgcagt gactttattt ttgcggcagc acagcgttag gataaattgt
     2341 ggcttcgctt ctttgacgtt aattttatcc acgaatgaat gttcgacaat gttggttgca
     2401 gtttcgtcgc acgccaaaat aaagtcggtc gcagtctcca ccgaaacgcc gtggtctttc
     2461 aatgcagatt tcacaaaatc atgcacaatg gggattgccg ctctttcggc ggtaaaattt
     2521 ttctttagac gtggcatact catcgtaaaa ctatttcagc acctttacta ccatgagagt
     2581 aaaatcgtcg tactgggcga cgtttcccgc aaaagttttt acttcacgaa aaattttctt
     2641 cgctaactgg tctgaagata gaagcatgtt gtcgttaatg actcgacaaa agcgttccat
     2701 tccaaactcc tcttcgcgat cgttcaatgc ttcaacaacg ccatcggtgt aaagaacaag
     2761 aatatcaccg acgttcaaga atgcgctccc ttcaccgtaa gggccatgcg tggccgagtc
     2821 gataacaccc aagggacggc cctttgtacc aagtttttca aatgtgtgtg tgtcgtggcg
     2881 gaaaatgagt tgttcgttgt gccccgccga tgaaaaataa aggcggtgtt cgggggcatc
     2941 atagaaggta taaaaaagtg tcacgaacat ccccgactgg ctatcctgat agatgaggtc
     3001 gttcgctttc aacaacacct cggcgggggc gtgtatttcg cgactaattg tgcggaggat
     3061 ggaactggtg acagccataa aaagtgcagc gggtaaactt ttcccactca catcagcgac
     3121 agaaaacatt acctgatcgc catcgaacga aagaaaatcg taaaaatctc cacccatgag
     3181 tttcgccgat tccgagacca cgcccatgtc gaaccacgac gatttttgaa agcgtgtcgg
     3241 caaaatagac ttttgaatac tcgaagtaat ctccaattct ttttcgtacg atctcttctg
     3301 caacatttct tcaagaaggc gaaaattttg aatactgcga gtaatttggc tcgcaatcgt
     3361 ggcgactaag cgcaagtcgt cgtcgttgaa ctcgtgagta agcgtcgatt gtcgatctga
     3421 aatgttgagc acgccataaa tgtcgcgccc atgcatgagc ggttgaataa tgcaaccacc
     3481 agtatgatac ctctcgatat tgcgaaaagc cgagtatgga gttttttcta atgcatttgt
     3541 ataaatgact ttgctggtct catacgcttt accggcaagc gatttttcga gcggcacaaa
     3601 actacgatca aacggtgcaa ggttgagacc gtggtgcgac accacctcaa gcgcatcgta
     3661 ctcagcagaa taaatcataa tagaaacgcg atctgcctgc atttcgtcgg cgataatacg
     3721 cacaatcgaa tcgaataaat ctttttcatt gagcgttgac atgagcactt tgctaatttc
     3781 gtgcagactt gaaagttcgt gcactttttt cttgagctcg cggttgatgt ccttgagcga
     3841 atcgatgagt tcgcggttgt ggcttgccaa cgccgcttgt tcggaaaatg tattgaaaag
     3901 ctccaagtcc ttttctgaaa attcatcgcg gccgattgag ttgatgactt cgattacacc
     3961 gatgatacgg ttgcgcacca tgagcggcac tgcaatcatg ttttcagtca caaaatttat
     4021 ttttgcgtcg gcttctttga acacacgcgg atcgttttgt gcgtcgttca caataagcgg
     4081 tttacagttc tcagcaacga tgcctgcaat ccccacaccc aaaggaatac gaatctcttt
     4141 taagtcgcgt tctttctccc ctgaaataac gttaaaaaac agctcgttcg tttttcgatc
     4201 gatgagcatg agcgaggacg cctcggcttt caaaacgatt ttagccgaat ccataatcga
     4261 ttccagaagg ctgtttaagt cgagtgtcga gttaatcatt ttcgacactt taatcatttc
     4321 agaaagcaag aagttgtttt caacgttatg gcggtaaaga tacaaatttt caaaaatagt
     4381 caccaagtta ctgctaaact tcacgagaag cgcagtgttg tccggcgagc ggatgcaact
     4441 ggccaacatt cgagaaaatg tacgcgcaaa agtactcgcc aaacttaaat cttcgggcga
     4501 aaacaattct tcgccttgtt tggtcaccca taaaagagaa cccaacgccg ctttatcatc
     4561 ggtaataagc ggcgctgcca agtatgttgt ttccgcttca cccatggaaa cctgcaccga
     4621 gttaaatagt cgttgtcttt tttcaatggc aaacgtctcg ccttccacaa gacttttttg
     4681 cataatcttt ttttccaaga ccgtaagttg ctgcatcgcc gtgcgatacg ttttcacccc
     4741 ttgcgcggtg cgtaacgcca agtaacagtg tttggagtgc atgagcgaat tggccgcttt
     4801 taaaatatag agcgtcgctt tattgagttc tgcggttgct tcaacgaata cacgatcgtc
     4861 tttttgttgc aataagtggg tgaagcttga cgaatacgtt gttaattctt cactactcat
     4921 ttttaattac ggtataaata gtagaaaggt ctgatttttg ttttattcac agctcctttt
     4981 gtaaatgcta cttcataagc gtcttcaacc gataatcctt tttcaaccat taaattgact
     5041 atcgactgtg caaataatgc aaaactatta tacgactctg tgtcgagaca aaaaagatga
     5101 tacgccgccg gttttaaacg cggtaaatca tctaagctga tattccgtgt ataaatattt
     5161 agcgcgccca cagcggtgtg gaaaggaggc ttgatttgta ttaccgccgc cttctcttta
     5221 tcacgggaac tcccccatcg cgcatacagc gccgagtata gtgtcgacgc ttcaggcgcg
     5281 gctaaagaaa gagtttggaa atcaacgttg ttttttgaac tcgagagaga aacgtcaaaa
     5341 agcaccgctt ttcgtggtac aggaatttta tgccgagcaa aaagattacg gcgtggcgta
     5401 taagctaaat attttcgaga acgcgataat tgagtaaaat atttttcatc gaattttttc
     5461 aaacgcgaaa atttaatttt ctggccgtct ttgaaccatt catatacttc gtcgtgaaaa
     5521 acttcgatgt acccaaaagc gccatctaat aatgaggtta tggcaacatt cgctttgcct
     5581 tgtgattgca gagcaagttt taacgcctta ttgttttcat cgagtactaa atacctttta
     5641 ataaataacg cacacgaagc gttatcgcaa ttaatggctt caaattcatc gttttctttt
     5701 gttgagacat agaactgaaa atcactttcg atttttttat cggcgacaaa accggtcttt
     5761 tgccacacgg ataaaatctc atcgttggga ttgactgtca aggccgcgaa gtataaatta
     5821 ttatacgccg attgcaaacc gctgctccat ggcgttgttt tctttttagc aatttcatct
     5881 aacgccgaaa cacctttttt agaaacatct tctgcgagac tcaataattc ataaaatgtt
     5941 tttgcttgaa agttttttgc tttttttgag gcatttttac catactgcgc caaaatttgt
     6001 gccaactgtt taatctcttc tgtattcgct tggcgtgtcg cgaaaacacg caaaagagaa
     6061 aatatcaaca aattctcaga gacagagagt tctgtacgta aaagatattt ttttgcaaaa
     6121 tctaacgaat caccgatata gccgcttttc atgagcgata atgtttgtaa aaaatctaag
     6181 ttgtccgcgt cggcaggtat ctgtaaattg atttgcgatt cattaaacca tctacgaatc
     6241 tgaagcctgt tttctttatc ttgttctgtt gcatttttta tagaaacgca agcgttggca
     6301 acaagagcgt actcaagcga ggcaaccgca gcggtacata tatcgttcaa catttcactg
     6361 tcttttaatc ccgcaggaat ttttttgcgc agagtttttg ctgatgggct aacgcttcta
     6421 ccccacggaa aatcgccggt taaattttca aaattaccag actcaaacga agatagctga
     6481 tacaatcctt ctgcttctgc actttctcga cgcaacgcat tggcgactgc atcatcggct
     6541 tcaataaacg cacgcgcttc gactaaacta cccgcacgca agagtaaccg cgcataactt
     6601 ttacgagcgg caagcggagt ccattcagaa acattcgcca tcgatatata ccgtttagcc
     6661 gcttcgttat tgccgccaat aaaattcaac tcagcggcca gaagttggta atccacattg
     6721 tctggaaaaa acgaaagcat atcttgagcg tacgatgtag caatttcata gtcacgcttt
     6781 tgccgcgctt cgcgaatttg ttttaagtag taagaacgct gccgctcaaa agatttttct
     6841 tcaacggcac tagtgtcgat ggaagcgccg gttacaagtg cagacgacgg agcatcgtcg
     6901 acattgaggt acgattcgat aactgtttgt gagtctttgc gtgaggcgaa cgtataaagc
     6961 acttgccgtg cgcctaacgc cgaatagtag agatcggaag cagcagcaaa acggaaatta
     7021 tctgcatcgt tcattttatt tcgagcgaca aaagaggcta ccgcgtaatt tggtttccca
     7081 cgcacgctaa aaagctcggc aggagttcgc gaggctgtat ctgcaataac gccttttttc
     7141 accagctcgc cttcgtagtc gataacatta cggtggattt ttaagtcgag aaaatcgttt
     7201 cttttactta cataatcgac cgttaagtta gttttatagc gcacttcatt agacgacgaa
     7261 aatcctaaaa acgtacttgt ttttacaaac tggagcacat tacgttttgc ttcggcaata
     7321 ttttttgaaa agttgatagc agagcgtaat gttgtttgat tatcgagcaa gcgaacgctt
     7381 atgctttcca tcgcagcggt taaaatttca ggagcaagca ctactgtttt tgcctgtgtt
     7441 ttttgaagaa gttcttcaag aacaacaatc gcatcgtcat ccgcctcaac aggggtggtt
     7501 gaataagtaa tttctttttt tcttccaccc tcagctttcg cgaaaatata aattatatcg
     7561 ttacgtggtt tcaacgcgta gataaatcca ccgtccgcca aactttgcaa atcattgtct
     7621 gaataaccca atgaaaaaaa agtgttgtag tggtacccaa gcgcatccgc ctgttgtagt
     7681 tggccacgta attgctctac acgattatcg attgcgacca attctttttc aaggggtgca
     7741 acatttgtgc gtgcgaagcg ttccaattca atttgttttg ccagcgtata gcgtctctgt
     7801 tctaaaacga gcaatttatc aaaatggtcg tccttcgtat tgtcaaattt caagttgtca
     7861 aaatagactt gcaatccgag catttggcgg cgtaaatcct cgacacgtat tgcttctgca
     7921 tagtttcgag atttaagagc gcgatcgatt acaatataac taatgcgctc aaaaaggtcg
     7981 ctattctgac gcaaaaaatt ctggtgcttt aaaaagaagt tgaataaatt tttgtactgt
     8041 cccaagtttg cattttcttc catgcccgcg tcttcgtaga tgcggtacga acgataagcc
     8101 gcaatggcat aaattaaatc ggcacggaac tcttggctac gctcttcaat ttcttttatt
     8161 tcggcaatag cactgtcgac aagactcatc tcttgcaaaa cctttgcgcg attcattgcc
     8221 agctttaacc gtagcgcgtt atttttcaaa ctgggctcgt atactttgtc gatatttact
     8281 gtgctctctt gcgtgtatcc acgaaaataa ttcaacaccg aagaaaaacg atcgaatgta
     8341 gtttttttat gcttgagata atcatcgaat gattcttcgc gtgcctcaaa acgctctctg
     8401 aaatcgtaaa gtatcgctgc atgcgctaaa ttcatcgcct tgaagtataa aatcgactga
     8461 atctcttgtt gcgccgcaag agtcaacttc gtagcacgtt cgtcggtgag ttcgcgaatt
     8521 tcgccttctt cgagctcttt taatcttttg cgctctgctt tgagacgggc agcgacaaag
     8581 tcattctcat actcttgtac ttcggcagca tttctttcgg cggtctttat tgcgcttgca
     8641 atcgactcgt tgcctgtttc aagtttacgc aatttacaca acaaaatatt tttacgaaat
     8701 gtttgggcgg cgtgtacatt tttatccttt tccgcttgtt tgaacgcggt ttggtaaagc
     8761 gacatcgcgc tatcccaatt ctggagttga aactctaact cgccacgccg actcaccgcc
     8821 gctgccaacg tttctttgcc gtgcttggtc ttatctttct ctgcgtactc caccagcttt
     8881 gccaatgaat cggatgcttc ttggatgaga cctttgtgct tatacacatc ggcgcgtatc
     8941 gaatataaaa gttgataacg atttactgta ggtaactcaa aagctaagtg attttcaccg
     9001 atgtaaatat catcgtaact cagtttgtaa atccatccaa cgatagtagt ccacggttca
     9061 taaacccaaa gccatttgat gcggatttta aattttggaa tagagatttc tttttctttt
     9121 ttcaattctt tttcagcgtt attcgtttgc cataaagccg cgccatagtt atcctgtttc
     9181 aaataaagac gcgccaactc caaatgagaa agcgaacgcg gcgtatcgga tgaaatcgat
     9241 tttgcttcag agaggatggt gcggtggtag taaatagccc gcgatacttg tcctgcgtat
     9301 tcgctgttga gagcaaacgt tttatacgca atggcgcggc gggcggcgtt agttttttgt
     9361 atttcagcag aaactccggc aatggggtaa cgcgagttga gattgtcttc gacaaattga
     9421 agatagcgca tcgccacttc gtttttgcca gagaagtaaa gcgtacgtcc gaaatgatag
     9481 taaaacatca tttcttgctc gggtgtttca aagcgaaaat ctggatttcc ttttttgtct
     9541 aaaatagcag cgtagtgttc ttcggcttgg ctataattat tgagtaaaaa ataattattt
     9601 gcaatgttta agtgaaagct attcttaacg cgctttgaag ccgcgttggc aaaaatcgtc
     9661 agtgtttttt gataaagtcg aatatttttc tcgaataagt aatcgggaaa atatgctttg
     9721 tacaacgact caaaaatctc acggtccttt ttcccagaag acgcgtctaa cacaacctca
     9781 cgtttttcgt cgatgaattg atacatccat ccaagcataa tgtacgcatc ggcaaagcgg
     9841 gcgttggcaa aaaaactcca gttgaaatca atctccgctt tgagaaaatg aacaaacact
     9901 tcttttttga cagacttggt gagtccttcg cgctctgccg cactatgttt attgataccc
     9961 aactgcgcac ggaaataacc gcgcccaaaa ataaattcgg ttactaaata gcgacgcgct
    10021 aaatctatgc gagaatcata aaattgaata atgtcttttg ccagcttttc gtctccgcct
    10081 tcttctcgcg ctgcacgcag cgcaatgtcg tctaagttaa gcgccgtctc aaggatttct
    10141 ccggagttta attcgctcgt tagcttagca gcatgcgtcg acaaaaataa atccaagagc
    10201 gccatgtatt cggtagcggc agcgcggtcg tttcgttcgc ggctaaattg agccgcacgt
    10261 tcggtgtgat aaatgcggtt cttggatata atcagttttg catattgtgg aatttcagtg
    10321 tcgcgaaaat tagcaatcgc cgattcgtga atcttaaacg cagccgctgc gccttcttta
    10381 acctcggcga tacgcgccaa ctgaagttgg ccatcaaagt agttaatcgt acccttagtc
    10441 gacaattcaa gggtgcgttt agcttccgtg ctcgcctcgt cgtattcgcc ttcttttatt
    10501 ttagcgcgtg ctttttgtaa tgcaaacatc gcatgcaaca tacgataatt tttcgttggt
    10561 aaatcgacca agtattgctc ttgcacaaca atgtttccgc ccgcaaattg agttctataa
    10621 aacgtcgaca gaaaacgctc tgccttacgc cgcactgcaa cccgaatcgg tatcgtttgt
    10681 ataggtagtt tactaaagta ggcaggtaaa agtttagcat cgggtttctc ggcgagcaag
    10741 aacacaattt caggtgcagg tgtactcgtt gaatcaagaa taccgttttc taaaaaaaga
    10801 gtatcaagtc cgatatacgt cggatttaat tgtaatattt tgcggataac tgttactgct
    10861 tcgcggtgtt taccttcgcg gattaaaacc tttgcttgtt cgtaacgaat aaacgactgc
    10921 actgcaataa acctgggatc aacctgttta ggttttatag attctacttc agattttatt
    10981 tccgattttg aatcgatggc ttcaggagaa attacaggcg tttgcttttc gaattcgata
    11041 ggctcattat tgtagttatt ccacaaatct tttttaacaa gaatgcgact aaagtactct
    11101 gcatggtttc gagtgtcggc caatggtggc agcggtaaaa ctcctttaag atcggcgtaa
    11161 tcgggagaaa ttatcgacaa atcgttaaaa agacgataaa gtactttctc cttctcgccg
    11221 ctttgattca agacgtgttg gagtcgctgt gccgcgcttt tattgcaggc ggattcacgc
    11281 atgaaactca actcacggta caccactaaa tcgggtgagt ttgtaaatgc gctgggcaat
    11341 tgttcaaacc ccaaacacgg caaattttta ttttttgttt ttcgtatcag cgtagtgaga
    11401 tactcttctt gttggagtat atccccctta acgggcaccg caccagatag tggaataaaa
    11461 ccgatattga tgtcttcgcc tttaacttgc gctaaaataa tgctgccgcc gataaagtta
    11521 ctgacgcgtg aatcgaaaac actatcgttt tcggcggtta gcgccacgcg ctcattatct
    11581 tctacgaaaa cgctttcgtc gtcgacaaac ggctttgtgc tctcaaaatt ttttggctga
    11641 atacggatga tctggcctgc atcttttcta tcaagagaac catcgccgtt agtgtctctc
    11701 gcgatacgcg tgagcacaac cgccttcata tcgggcgtat aactggggta caaatcgagc
    11761 gctttgccag aggttaagcg gcgggttttg cccgtggcga tttctaattc atagaccgcg
    11821 ccgtttgaat tttgatcttg ataagaaata tagacaatac gtttactgtc gggcgaaaag
    11881 ctgggcatca caccaccttc tgtagtgagc tgccgctttt tctcgggact cgacgcgggc
    11941 ataatccaaa tgttttgcag cgcgcccgcg cccgcgccca agtcgtcagc tttctttaaa
    12001 ttgaggtctg tgctccatgc aagaaaatcg ccatcgggcg accaagcagg gtttgcatcg
    12061 cgcgcacgga tacggtgctt ttcactattg gttaaatttt ctgcacgcgg agtaaatctt
    12121 tcatcgtaag aagtgtcttt atcttgataa ttttgaatca acttctttgg attcacctta
    12181 agcaacatga tgtcgccgtc gggatcgagt tcatcgtcca cgtacgcgag atatttacca
    12241 ttggggctga ttgtcggttc gcgttggttg gtgactttag agacaacagg aatcgaaaac
    12301 acatcggcta aatcgcgcaa gtaaagatcg taattacccg ctgtgttcga ggagaaataa
    12361 agatatttct gatcgcgcga aatagagccg ctgtacgatt cacccctatc gattgttaca
    12421 ggaatgatac caccgtcacc gcgttggtaa cggtcctggt ataaattctc gtaactaaac
    12481 cgcgagtcat cgcgcttgtt acccgatgca caatgcgcgg ctaaaaacgc agcaaataaa
    12541 agtaatgcaa gccggcgcac gaaaaaatcc tactgaacgc gaaactcttg cgacgcaatg
    12601 acttcgccat ttgcgccaag tacatggact cggtaacgac ccgcatgcgt gaaattatct
    12661 ttttggaact gtgttgtaaa ataccgccac gtcggcttca cgctaccttc aacctgagca
    12721 acactttctt cttttatcat attgcttgca tccgcataaa cctcggtgag ttttgcgata
    12781 attttatctt gccccaaacg cccaccgctg gaaaaaagca agtagagagt tttgccgcgc
    12841 gcaaaaacta aattcgtcgt gcctaagtgg cgcgtattgg cttcttcgat ggcggtgttt
    12901 gcaagataaa ttgtgggttt ttgcgcggta gaaaaaatta cccagtacaa aattgtcagc
    12961 gcaagcaacc ccaccactgc aaacaacgcg taacggcgcc aacgcggcgg ttcatcgtcg
    13021 tcggcaaaag cagcaggttg aacggggctt tgctcccccg atgtgagacg ttttaagcga
    13081 ctttccataa ctgagccggt tcttttttct tctttttgtt ccggcaagtc atttttgctt
    13141 ttttgatttt ctgccgaagc gccgtttttg gattctgaag catagttatc ccctgtatcg
    13201 ccgcgcttgc gttgcatatc gctcacaaag tcacccaacg ccgaggcgtc ctcgcggttt
    13261 tctcccaagt cgtcggcgat cgctttgcgg aaaatgtctt ttttattttt tttcatggct
    13321 cgtgttgtga cggcataaca aaaatggttt tgagttcttc ggcgagtgca tcgtacgctt
    13381 ttgcgacttt attttcggga gcgtactcaa aaatatcttg gcgtaacaaa tgcgcttctt
    13441 cgaccgccac cgaacgaggg atcgtcgttt tcaaaattgg taaaattttc tccacctcgg
    13501 gtatcatcgc ctgcgaaagc gtagtgcggt tgtcgtgcag cgtgaggcat ccacccgcta
    13561 ttttgagatg cgcgttgtag cggcggttta ccgtggaaac cgcatcgcgt atattttgga
    13621 taccgtcgat ggaaaatttg gaaatctgca acggtacaag taaaaaatcg gcagcaacca
    13681 tcgcgttgat ggtgattatc gaaagcgacg gcgggcaatc aaaaatgata taatcgaatt
    13741 cgtgccgcag agtttcgatt gcatcgttta aacgaaaaaa accgtctata gcgccggtga
    13801 gttgtgactc cacttcaact aaatgcaagc tcgaaggaat aataaaaaaa tttttaagtt
    13861 tggtgggatg cagcatcgca cccacatttt tttttccttg aaaaagagcg tacaccgaat
    13921 tttcaggggt aatctcacgg ctgcctaaaa ggatggaagt ggcgttgcgt tgcgaatcta
    13981 aatcgacgag aagaattttt ttttctgttt cacgacgggc aaacgcatac gccaaggcta
    14041 acgccgtcgt tgttttggcg acaccgcctt tttggttggt gatggaaaga acgaccgcca
    14101 atggaacaga ttaatcccac ttaaattttt aagcgtcagc caaaaatttt tctagccccg
    14161 cgtcggttaa aggatgtttt accaactgcg gaaagacact gtaagggatt gtggcgacat
    14221 ccgcaccgat gattgccgct tgctttaaat gtatgggatg gcggatactt gcgaccaaaa
    14281 ttttagtgac gaattcgtaa ttatcataaa ttgtcttgat gtcttcgatg agatccatac
    14341 cgtcggtaga aatatcatcg aggcgtccca caaacggcga aatgtacgtc gcaccagctt
    14401 tagccgccaa aagtgcttgg ttcgccgaaa aacagagcgt cacgttggtt tgaatccctt
    14461 cgtcggtaaa aacgcgcacc gcttttaatc cctcggggat gagcggcact ttcaccacca
    14521 cgttgtcggc aagtttagca agctcgcggc cttctttaag gatagcgtct ttctccacag
    14581 cgagcacttc agccgaaaca ggaccgtcga caatcgaaca gatttcttgg attaccgctt
    14641 taaacggctt accggttttc gcaatgagtg atgggttggt cgtcacacca tccaaaattc
    14701 ccaactcatg cgctgctttg atttcgccga catcggcggt atcgataaaa aattccatac
    14761 ggcaaagcgg tacacgaaac gcgcccgtca actataagag attatgagcg aaatggagcc
    14821 tatgagcctg tacgtcgact cgtgcagcgt gtataaatag gaaattttta tggaaaaatc
    14881 gtgttttttt ttgaataaat cactcatgtg gatagttacc ccctaatgag acataatctg
    14941 atttttttta agagaaaaat cccccacatc tccgacaata aaatatgcta cgaggcatct
    15001 acaccggcgc gtcgggaatg acggcgatgc aaaccaaaat ggacacggtc gccaacaacc
    15061 ttgccaatgt cgataaaacc gcattcaaag aagacgtgac gattttccgc gccaaccccg
    15121 aggtgttgat ccaccgcacc cgcgacaacg gtgtgggttg ggtgccgatg ggcggttttg
    15181 acattgcgcc cttggtgggt aaactcggca ctggaagcga agtgaatgaa atttttaccc
    15241 gctttgacca aggcgcattg aagcgcaccg aacgcgacca agatatcgcc attaacggcc
    15301 aaggtttttt cgtggtgcaa agcgaagcgg ggccccgtct ctcacggagc ggtgcgttat
    15361 cctcgaccgc aacggcatgc tcgtcactcc cgatggattt ccgctcatgg gtgaaaaagg
    15421 accaatccaa gtcaatcata acaactacat catcaagtcc aacggcgaag tgtggatcaa
    15481 ccaagcggtg gggaataatc cccgtaattt tacgagtaaa gacatcaatc agtgggaaaa
    15541 tccagtattg ctcgatacgt tacagatacg cacagtagat cacccgcgcc acttggataa
    15601 agtaggccag tcgttttaca acactactcc cgaatcgggc gatatgcgtc cggtggatca
    15661 agcaaaggca cccgaaatat tgcagggatt tttagaagcc tcgaacgtaa atatcgtgac
    15721 cgaaatggtg aatatgattg aagtgcagcg gatgtacgag gcgaatcaaa aagccatcac
    15781 cacgcacgac ggtatgttgg gtacactcat caatcaagta ttgcgggtat agttaccacc
    15841 cttttccacg acaaacgcta ctaacaaaac gattttcatt cccctgtagt ttctcgatga
    15901 gcgtagcgcg gcgacactcg gcctcatcga tgggatcttc cctgtcccac gcctccatga
    15961 ggcggcgcat tttgtcgcct aaaataccac gattcgggta tgcgtcgttc atgtaaaagt
    16021 aaactcgcgc aatatcccca cggatggatg cgcggggttc gacaattttt tcatcgatat
    16081 aaaactcgca tgcgccatac gcttgtactt ttttgctcac aataccataa cggtagttac
    16141 tccggtcacc gttgagttcg ccaatcgagg cataaagatt atagaggtct gcctccatac
    16201 gccggaatgt ggtattcact ttgcgcgcac actgccgccc cttgaaagta cgaccatcgc
    16261 ggcgcacaca acgttcatcc ccctcgcgcc actcgataaa actgcgccca aagttttcgg
    16321 cgggcacgac gtgctcccat tcgatgcggt tggcgcggga gttatcgcgg cgcggaacat
    16381 agccacaact accgcgttgg atttcacgtt cttcgatgcg gcagttgcaa taaaacgtgt
    16441 aagggtgttt ttcgataagt tgccacgcag aacgcttcgc ctttgcaaaa gataaaatag
    16501 tcttgttgcc gccagccgat agcggcaaga ccaaataaag aagaaaaaat atgtgaaaga
    16561 aataccgcat tacgatctaa agtttgcgca accaacccaa gcggtctttg aggagcgctt
    16621 ggtcgatctg cgcttgcgcg tcttcaatca ctttacggtc aatactctgc gcaatgcgct
    16681 cggcgcgttc aatttctgtt tcggggagga gcgctaaaag ccgcaatcca aaaggaatca
    16741 atccttcggc gtgggcagtc agaatcactt ttttcaaaag cgcatcctcg aaacggtgcg
    16801 acatcgccga aaggcgttct acatcgcgct cggccaaatg gtctgcaatt ttgagagcgg
    16861 tggatagctc ccccgattcg tccgctgctt cgatgagacg cgccaacgct tcggagtcga
    16921 gtttcgcgca gatggtcgcc atgtgcgcgt acatatcgcc gctgagatgt tttaagaaag
    16981 caaaaatata aaccaacgct ccgtgctgaa tcgcaaacgg caccatacct aagagcgcat
    17041 ccaccgatac gcgccccaca agacgcgcca ggcgttggtg ttgttcgtcc gtgaaatacg
    17101 ccaagagacc aatcaccgca ggccacaagt cacgccgctc agcagtttca accatatcga
    17161 cgatcttgtc gtcgctgaaa gaatcgacga gcttcgcgac gtgcgcttta ttttgggtgt
    17221 aaagtcccac gcgcaacaac gattccgtgt cggggatttc tttcgagaga gcgttaattt
    17281 tatcgatgtc catataatcg acgaacgcgc ccatcgtgaa aaattttttc tcgcgcgtta
    17341 aaacgcgtgt gaggttgatc aacatatcca tggggaatgc ttcgataata tgcttggcgc
    17401 gcgctggcac taaattctcc gtaacctcgg ccaagaaatc aatatttagg cttttagcaa
    17461 tagaaaccgc ttgtttaaca tcgatgtggt aagtcaggtt agcagtgatg tttggaccca
    17521 agatagattt tgctacttgt gcgttgataa agtttggcat gaacttcgcc acgctcgcga
    17581 gcttcgacca cacgttcgag gagtcggtga gcaagcgatc agaaatggct tggttgagtt
    17641 cttgaaattc atcgatcgat aaatttttca aaaactgtag tttttcaggt gatactttta
    17701 aaacgtgtgc cagcttttca acttcggtaa gtaaatctaa actattcaca aatgaaccat
    17761 tgttcactat tgcggtgtta tggcgagcga aaataaagaa gtgtgggcga tgcaagattc
    17821 gaacttgcga ccccctgctt gtaaggcagg tgctctaacc agctgagcta atcgcccttt
    17881 atttttcatc aatcaaccat caggtgctct aacctgatgc gagcgtcttc gctctccgga
    17941 gcaaggccgc gccttacggt ggcgctaatc gccctttagg aacagttatt cgatttttta
    18001 cacgcgacgc acataaaaaa tcgaaaatat taaacagcgg gagttgttgc cttctttaca
    18061 aggtttgcaa tgcgcgattt ttttcgtgct gccgcgtttt tatggactaa gtttgttttt
    18121 gccgctttgt cgactaaaga agccaattta ttttgcgcta cagtaatctc ttctactttt
    18181 ttttcagcaa ctaatttacg caactttttt tcttgtgtac gtatctctga tttttgtgac
    18241 tgattttgac gacgacgctt ttcgttttgg cgtgtgcgtt ttttggcaga agctatattc
    18301 ggcatattat tttcctaatc cctaaagtag cggcaaagtt gggttttgaa tcatcgcgtc
    18361 aatcgttagt cgctacttaa agactgctgc cttgtcggtg ttttcccaag taaacgaatc
    18421 ctgtcctgtg cggccaaaat gcccgtacgc cgctgtgggc cggtatttga tattacgcaa
    18481 atttagcgtt tcacggatac cacgaggggt gaggtcaaaa ttggcggcga taattttttc
    18541 aatttggtcg tcgccgattt taccagtacc aaaagtattc acaaccaatg aaaccggttt
    18601 ggccacacca atcgcgtacg ctacttgaat ttcgcactgg tttgcaatcc ccgatgcaac
    18661 gacgttcttt gccacatagc gcatcatgta cgctgcgcta cggtcgactt tcg
//
LOCUS       DAOMPD010000004        16844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1497_length_16844_cov_3.117398, whole genome shotgun sequence.
ACCESSION   DAOMPD010000004 DAOMPD010000000
VERSION     DAOMPD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_16844_cov_3.117398"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1520
                     /locus_tag="PLY93_00335"
     CDS             165..1520
                     /locus_tag="PLY93_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP67967.1"
                     /translation="MKLFPLLTISSLLVSGSPFFATEKVDEAKVIELVREKNLSLIIE
                     KFNVDIAKAERVQAALWANPSLGLSSTLNPFHKEYEQTTSAGPRQLDLALAIPIDANG
                     KRSLRKKSADIAIRLAEALYVNKTRMVIRDSRLAYVQALYLRDKLNLVQQKKNAMQSL
                     VRILERRIGTASGGRQPLLLDRTRLAVENTLVEERLVDTQYAAILVELRILCGYSGQD
                     NLAPTGILRIKNSPLTDSLPQLIEKARANRPDLRAALLAITFSKEQKDLALAEKWDDI
                     EFSLGLTRQLGFAANPNDVDALGNVQTKSLPGEYSFGFGVRIALPIIDRKQGNVEKTE
                     QQIKQSEFASRALEREIENEVIKAYNKIVATRASLSEYEKTSLKRAQRVKDAQQRLFG
                     TGGVNLLEYFDSYGAYFDTISGYYETASEFRKACIELETAIAAALEGEATVKEANDAS
                     R"
     gene            1507..2316
                     /locus_tag="PLY93_00340"
     CDS             1507..2316
                     /locus_tag="PLY93_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67968.1"
                     /translation="MLPAKWKSRTFFTVVVVCTTLAVWLMLPKLFQTKDRTPKKNYEI
                     TSNNTIRFLDSDEFLSSLVIKEAVAEPKDNYKLSSVGQIVLLIESGMVATGERLHPLH
                     LDENYSKKIGLNSLSWRAGNAFVVSEISSAYKIKPGMPIQIARYGVAIADSRAVVRRI
                     IKNKDDSESITLIFEIFKGQDWYPGANCKLIFPTLHEKPVAIAKRALLHLGDKDYVFI
                     EKSFGEFKLQTIHVVDEGDIALSVTGLLAGDRILTKGSILLKNFIPELLQR"
     gene            2322..5498
                     /locus_tag="PLY93_00345"
     CDS             2322..5498
                     /locus_tag="PLY93_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRP67969.1"
                     /translation="MSFIEKSVKAAVHAPFIAILIAACMGGLGIFSLTRLNIDAYPDI
                     SGVQVQLITELSGRAAEEVEKQITIPVERGMLGIQGIEIIRSRTIFGLSLVQILFREG
                     ITDHFAREQVYQKIGELDLPDGVKPSMASLSTAYGEIFRYELVHTQKQDPMELRTLND
                     WVVIPRLMQIPGVVEVANFGGMGKRYAIHLDTQKLLQYGIKLQEVIEAVQANNSNGGG
                     SILERGSTALIIRGLGRINDYHELEKIFVKNVYGTNIFVSDLGKVVVDHPPRTGVFGK
                     DENADSVEGIVRLRRGENPSQVLKRINDAVKDINVTTLPKDIQIKPFYDRTQLIHETV
                     MTVVENTAVGILLVIATLAIFLKDIRMALIVAVTIPMSLLFALLLMYFWNIPISLLSV
                     GAIDFGIVVDGAVIVAENIIRRMGHRTHFSEREFGEHIYESAVEVQKPMLYSMLLVII
                     AYIPLLSLSSIEGLLFRPMAITLCFALLGALLFALFLVPSLTNYFYRKKSNIAEKPEG
                     ALFSRFKEFYARTLPVLIQKRKRIVVIFLSLFLVIISVIVPNLGTEFLPYLDEGNFWV
                     RANFPEGSSLSETSEFAAEIRTLVREFQEVSFVSSQTGRSDAGNDPFPMNRIEFMVGV
                     KPRSAWKNYKTKLDLEAAIRTKLQKRFPTLRLNITQPIIDSVTEDTNGTSANLAIDIM
                     GGDMDTLRQIAKNTVALLRKIPGNENVNIEQEAPQPQLQIKIDKEKLARYRLSAQTVN
                     TVVNTAIGGLPVSEIYEGERRFNILVKFRPSQMKSPRDISLLPVFNDHGEAVPLGQVA
                     AIRVIDGETLIARAKNMRRLTVRCDIRNRSQGDFVADAQHRFKKEIQIPAGYSVEWLG
                     MFENLARARNHFALLIPFTVVLIFLLMLAIFRSVGKAALVIMTVPFAMTGSIVAMAVR
                     GMHFSVSAAVGMTTLFGIATMHAILMLSYIAQLEAEGRPRLEAIIEGAKLRLRPVLMT
                     AVVAFLGLLPASLATGIGSDVQRPIATVMVWGIFSSAFLTLFLLPVFYALFFPIDIVT
                     PEVMPVSEEIPTKAKFNGRRKVSKKLGS"
     gene            5495..6100
                     /locus_tag="PLY93_00350"
     CDS             5495..6100
                     /locus_tag="PLY93_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRP67970.1"
                     /translation="MKLYGSITSPFVRRLRFVCNELGVNFTFVDTATDAGQAEMRTKN
                     PIWKVPCVEVDGQILWDSHTIIEYISEKNSGHPKLLRTPQGAEKWREKNLVAAADSCV
                     ESAINVFYLNKDGVKVNEVPYMVKQKARVASILAWLKTQLHDNYFTAEKKIGLSELTL
                     YCILDWMRFREMYPVNDDAALKAYLAFHANHPSIAQTKLPG"
     gene            6048..8006
                     /locus_tag="PLY93_00355"
     CDS             6048..8006
                     /locus_tag="PLY93_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRP67971.1"
                     /translation="MRSTQTTPASLKQNCRGNSNTPPAHADNLTTVRHLKLGCYLFFL
                     STALFADSGSEIDPVHNVLMHIGLAIFASAVLAYFAFLAKQPVILAYLAAGVLLGPNF
                     GLGLIAREKDIATISEIGLILLLFMIGMEIDLKKIKASGKSLITSGVTQFAISVALGL
                     GFFALLGYSFSGSKYDLFYLAVCCGISSTAIVVKLLYSKFELDTLSGRLTLGILVFQD
                     IWAIIVLGVQPNLANPQILDILLTFAKAGALVAASVAMSKYILPYAFSRIAKIPELVL
                     LTSLGWCLAVCAAALALGLSLEMGALIAGVSISTFPYNLDVIAKITTMRDFFITIFFV
                     SLGMKIPHPIENLSLVGMAFIVALFLIVSRFLSIYPVLFSLKNGNRVSILTSLSLANM
                     SEFSLVIAALGLASGHIDQNLLSIIIFTFVITSILAPYMIKYNHQIQLFLTRYLEKLG
                     FRDRAKFEEPPASTLDKEIAILGFFRVASSLVLDIHNEKSKDSLKDKIVVVDFNQAVH
                     EKLHEYGVAAVYGDIAHVDTLHHIGLHDTKIAVSTIPDNILVGTSNTKLVKQIQDIAP
                     HAKIVVTAENPSSALKMYEAGADYVFLPRMVASQHLMPILRDMLAAQDSEDKSKTAVA
                     KRKKAIERLTKLKKAEMDFLRQRPEVIK"
     gene            8029..9660
                     /locus_tag="PLY93_00360"
     CDS             8029..9660
                     /locus_tag="PLY93_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRP67972.1"
                     /translation="MIIRGDNVAKKYFDALLLIVTLFAAIEVPLHLALAYETPHWVHI
                     INFVYPILFGSDIILTFFTTITVDGQEITSKRTIALKYLRSWFIVDLLAAVPFDLIFT
                     QGWPSEASNMARSLRLFSPRYINILLQVRMLRLYHIFPFLEKTTKKELFNPSIVRLIF
                     TVFIVLIVAHWISCGWLALGKVDATLDTFTNYIKALYWTMTTITTTGYGDIVPTTNAQ
                     SAYTIFVQLLGAGMYGYIIGNLASMLANSDLARTQFRAKMEKIQTFMEYREVPDELQE
                     SIRTYYDYLWNNRRGFDESSVLDELPASLKLQVAMHLNRDIIEKVPMFSNAPQELIQE
                     IVVSLKPMLYTPGDYICRKGEMGDEMYFISRGRVEIVSEDGATVYATLSDGSFFGEIA
                     LLFSSERTASVRAADYCDVYTLDKFTFDAMLAKFPDVARQVQVMAEERKQRAETENRN
                     KASVDASTAIVRELIARVSQRYVFLDWHTVAGADGYQVERWDDAAGGWNFLSKSVSIS
                     EFYDVIPLKTTNTYRVRAILGTEFGPWSHAVHIDV"
     gene            complement(9657..10286)
                     /locus_tag="PLY93_00365"
     CDS             complement(9657..10286)
                     /locus_tag="PLY93_00365"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRP67973.1"
                     /translation="MFNVDALVGWIQSIHEPYSYLLVFAVLLLCGFGLPIPEDITLIT
                     GGLMAYYGKADVYVMIVVGLVGVMLGDGTMFHIGKRYGYRVFEWKWMAKVMHTDRIEL
                     VKHRLEHHGNKVIFSARFMPGVRSLVFLSSGALGIPFRVFFLYDGLAALISVPAIVYS
                     CYFFGDQIHHAVEVIRSVENGILILIGLGILYFVVKYFIKKRREKAETY"
     gene            10415..10648
                     /locus_tag="PLY93_00370"
     CDS             10415..10648
                     /locus_tag="PLY93_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRP67974.1"
                     /translation="MDLSVRVYEKEGEFVASCPELDVYTYGVDAEEARRRLKHVIAFY
                     ASTAQDLGYKIDASELLSNLKAPAHTRSESFLN"
     gene            10699..11604
                     /locus_tag="PLY93_00375"
     CDS             10699..11604
                     /locus_tag="PLY93_00375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRP67975.1"
                     /translation="MPEALPPRILIFDDSEVERLYLEALLKNEFHVETAKDLIDFWAH
                     IAEDAPDLILLDVYLPQISGFELCKQLKKNSEYASIPIIFVTSLDNANDIEEGFDVGA
                     HDYVTKPVMQRELKARIRAALRIKHLEQELRLRSVTDYLTGPYNRRYFYEVVIANMSY
                     AIRMRRNLCIAMLDIDFFKKVNDNYGHEAGDAVLKHFTGTIKYQIRKYDILARYGGEE
                     FVIQFFDCTLERGLEFLNRIRDALATSPCVYESHTIYYTFSAGIVSLEEVDADSPLER
                     MIEMADKRLYSAKKSGRNRNVSIDV"
     gene            11642..12532
                     /gene="cyoE"
                     /locus_tag="PLY93_00380"
     CDS             11642..12532
                     /gene="cyoE"
                     /locus_tag="PLY93_00380"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRP67976.1"
                     /translation="MRDLIRLAKGGIIFTVVITGFTGMLMAKRWVPPVWDVLWVVASL
                     TLSSGASALMNNLFDYDLDKNMKRTLWRSGLIDRFGKRNLWILAAVASVLSLVPLIVF
                     QRWWALFFTVAAIFSYAVWYTIYLKRRGPFGAIVGGLPGALPVLIGAYAVSDRFAPDV
                     WLLFAFMMLWQPAHFWALALKIQGEYAKGGIPVLPLVYGNEYTRLYILLYGFSLPPLA
                     LGVGLSGGYSLFYLIASSVASVHFLYRTIRGIYPQTNKDANGEVAPPQYGRAFFASII
                     YMMVVMLLVIADIFLSPLFH"
     gene            complement(12529..12885)
                     /locus_tag="PLY93_00385"
     CDS             complement(12529..12885)
                     /locus_tag="PLY93_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67977.1"
                     /translation="MQSILCFFYMLRKLCSLFFIAFLVHPLYAYTDPGTGSLALQVFL
                     ASALTAWVSVKLYGRKIKNYFSHFFDKEQNDSVDEVPALKPATMQTKERYSRATNSAR
                     KKKAPAQKLSKKKSKS"
     gene            12888..14783
                     /locus_tag="PLY93_00390"
     CDS             12888..14783
                     /locus_tag="PLY93_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRP67978.1"
                     /translation="MLSLSIPSLIIFIASLLAFVFLEGLFFFTRPSFISLTEPATVFS
                     AVFVASFFVVFIGVFLWGFFWGLEFLLRRYLDSHLRLTLLPSAFVLALLALLLLDNFT
                     YTVFHIGIISTDILGRLVYVGLFSYTIFYLFHRLTFFVHEKKELLSSNHALLTLALPL
                     GLAFIFFMSKAAVPLKASLLETGVLTKRTRPNIIFFASDGVDAQHLKLYGYARNTTPT
                     LNRFAHDRKPLLCKNAFSNGARTTGSLTAMLTGVSPATNKVFFPPHILNATNAHRHLP
                     GILKQLGYINFQETIRYYADAGDLNLQGAFDVANNRSLKGQNFLGRRFVTESYFLAAL
                     FERLSSRLLHITGVRSMENFAAQMNNEDSPNEVYGTIDDTRMERLEGFIEQVKEPFFA
                     HVHLLDSHCCTFKFKHAHFSRRTAEEKETKTQPLERDLIDDAILASDAQFARVLKVLK
                     ERGILERTIVVYSSDHNRGWTIKRKVPLVFFFPDSHYARIEKKNCQLLDVAPTLLDYL
                     DIKTPHWLEGRSLLDEANLDNADRIFGFLNIRRHSIQVGKSKVSELVDAGAPYYGLTS
                     YGMMSCNKWYELNLNNGKMKSGKISGYSGGCEKGILPTELNAKKEIVQYLSSKKLGMH
                     FQKTSAK"
     gene            complement(14904..15392)
                     /locus_tag="PLY93_00395"
     CDS             complement(14904..15392)
                     /locus_tag="PLY93_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP67979.1"
                     /translation="MKLNKFFAIALIALVGFTMACGSTQKTEEVRSVSSGFGIDAANE
                     QLAKIPVVGFPPYQSSMPMSQFDKYGENAATVAKGVVATLPEGYKIQLTGHANANSGR
                     SAAFVKNISTQRAVFVKNYLVKKGLAANKLVTVGAGDSQQDPNLSKENNRRVTFSVVE
                     AK"
     gene            15563..>16844
                     /locus_tag="PLY93_00400"
     CDS             15563..>16844
                     /locus_tag="PLY93_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRP67980.1"
                     /translation="MSDWKNEILKNLNEPQQHAVQTTQGPLLILAGAGSGKTRTIIHR
                     MAYLIHVEKVPAYKLAAVTFTNKAANEMRERLLNVAGPIGSDSTVRTFHSLGLFLLRR
                     NAAALEYPENFSIWDDADQQSALSQILEKFEGKFNRTETRYFANSISSFKDRLVTPQN
                     LVDEVDLDAYEFGDILQEVYQLYELKKKASFAVDFADLISLPCFLFDKNPDLLTHWQN
                     RYPYWLIDEYQDTNFAQYRFVQHVAARDRNLCVVGDDDQAIYGWRGADVKNILDFSLD
                     FKDATVVKLEENYRSTKPILDVANAVIQNNLERMEKTLFTNRAGGTPPLLISTSDDAA
                     EARQIAALIQKAQQAGVPNSEIAVLYRTNAQSRLIEEACLNAKIPYRVYGGLSFFARK
                     EIKDILAYFRLIVNPHDETAFLRVINTPSRGIGEK"
BASE COUNT         4336 a         3958 c         3980 g         4570 t
ORIGIN      
        1 ttttgatttt tagaagggaa gaagggcttg gtgtgcgagt tggcgcacag gctccccgcc
       61 gagggtaagg atattctgga tgtctttacg cgctatgtta ttctataagc ctgcatggta
      121 agcgtaaaat ttaactctat ttttagccac ataggtagaa atcattgaaa ttatttccat
      181 tattaactat ttcttctcta ttagtgagtg gttcaccgtt ttttgctaca gaaaaagtcg
      241 atgaagcaaa agtaatcgaa cttgtgcggg aaaaaaatct ttccttaatt attgaaaagt
      301 tcaacgtcga tattgcgaaa gccgaaagag tgcaggcggc actttgggca aacccatcgc
      361 tgggattatc gtcgacactc aacccattcc acaaagaata cgagcaaacg acatcagcag
      421 gaccacggca gttagatctc gctttagcca tacctattga cgccaatggt aaacgttcat
      481 tacgtaaaaa atccgcagat attgctatcc gtctcgcgga agcattatac gtcaacaaga
      541 cacgaatggt catccgcgat tcacgcctgg cttatgttca agcgttgtat ttgcgcgata
      601 aactcaatct cgttcagcag aaaaaaaatg ccatgcaaag tttggtgcgc attctcgaac
      661 gtcgcatcgg cacggccagc ggtgggcggc agccattatt gctcgaccgt acacgcttgg
      721 cagtagaaaa tacgcttgtc gaagagcggt tggtcgacac acaatacgcg gcaatccttg
      781 tcgaactgcg gattctctgc ggttattccg gccaagacaa cttggcgccc acgggaattt
      841 tgcgcataaa aaattctcca ttgacggaca gtttaccgca gcttattgaa aaagcgcgtg
      901 cgaaccgacc ggatttacgg gcggcgttgt tggcaattac tttttcaaaa gagcaaaaag
      961 atcttgctct tgccgaaaaa tgggatgaca tcgaattcag tttagggcta acgcggcagt
     1021 tgggttttgc cgcaaacccc aacgacgtcg acgcgttagg caatgtgcaa acaaaaagtt
     1081 tgccgggcga gtattcgttt ggctttggtg tacgcattgc cctgcccatc atcgacagaa
     1141 aacaaggtaa tgttgaaaag accgaacagc aaataaagca atccgagttc gcctctcgcg
     1201 ctttagagcg cgagattgaa aacgaagtca ttaaagcata caacaagatt gtagcaaccc
     1261 gagcaagttt atccgagtat gaaaaaacat cgctcaagcg tgcgcagcgt gtcaaggatg
     1321 cgcaacagcg tctttttggt acgggtggcg tgaatctgct tgagtatttc gattcatacg
     1381 gcgcctattt tgatacgatt agcggttatt atgaaacggc atccgagttt cgtaaagcat
     1441 gcatcgaatt ggaaaccgct attgccgcag cgcttgaagg cgaggcaaca gttaaggaag
     1501 caaacgatgc ttcccgctaa atggaaaagc cgcacttttt ttactgtggt ggttgtttgc
     1561 acaacgcttg cagtgtggct aatgctcccc aaactgtttc aaacaaaaga cagaacgcca
     1621 aaaaaaaatt atgaaataac cagtaacaat accatccgct ttctcgattc tgatgaattt
     1681 ttaagttcgc tcgtcatcaa agaagcggtt gcagagccta aagacaacta taagctttcg
     1741 agcgtggggc agattgtgct gctcatcgag tcgggtatgg tagcaacggg cgagcggttg
     1801 catccactgc acctcgatga aaattactca aaaaaaattg gattgaattc gctttcgtgg
     1861 cgagctggca acgcattcgt cgtctccgaa atctcaagcg cgtataaaat caaaccaggc
     1921 atgcccattc agattgcacg ctatggtgta gctattgctg attcgcgggc agtggtgcga
     1981 cgaatcatca aaaacaaaga cgattctgaa agcattactc ttatttttga aattttcaaa
     2041 ggccaagact ggtacccggg cgcaaattgt aaattgatct ttcccacgtt gcacgaaaaa
     2101 ccggtggcga ttgccaagcg tgcattattg cacttgggcg ataaagatta tgtttttatc
     2161 gagaaaagct ttggcgaatt taaactgcaa acgatacacg ttgtggatga aggcgacatt
     2221 gcgttatcgg tgaccgggct tctggcgggc gaccgcattc tcacaaaagg ctcaatttta
     2281 cttaaaaatt ttatccccga gttgttacag agataagcgt tatgtcattc attgaaaaaa
     2341 gcgtcaaagc ggcggtgcat gcgcctttca tcgcgatatt gattgcggca tgtatgggcg
     2401 gcttgggtat tttttcgctc acgcggctta acatcgacgc gtatcccgat atatcgggcg
     2461 tgcaagtgca actcatcacc gaactctcgg gacgcgctgc agaggaagtc gaaaagcaaa
     2521 ttacaatccc cgttgagcgc ggcatgttgg gaatccaagg tatcgagatt atccgctcgc
     2581 gcaccatttt tggtttgtcg cttgtgcaaa ttcttttccg cgaaggcatc accgatcact
     2641 ttgcacgcga gcaagtgtat cagaaaatcg gcgagctcga tttacccgac ggcgtaaaac
     2701 cgagcatggc gagtctttca acggcgtacg gtgaaatttt tcgctatgag ttggtgcata
     2761 ctcaaaagca agacccgatg gaattgcgca ccctcaacga ttgggtcgtg ataccgcgcc
     2821 tcatgcaaat accgggtgtc gttgaagtcg ctaactttgg cggcatgggt aaacggtatg
     2881 ccatccattt agacacccaa aaacttttgc aatacggcat caaactacaa gaagttatcg
     2941 aagcggtgca ggctaacaat tcaaacggcg gcggcagcat tttggagcgg ggttcgacgg
     3001 cgctcattat acgaggcttg gggcgcatca acgattacca cgaactcgaa aaaatttttg
     3061 taaaaaacgt gtacggtacc aatatttttg tgagcgattt aggaaaagtc gttgtcgatc
     3121 atccgccgcg cacgggggtt tttggtaaag acgaaaacgc cgatagcgtc gagggtatcg
     3181 tgcgtttgcg tcggggagaa aatccgtcgc aagtattgaa gcgcatcaac gatgcggtaa
     3241 aagatattaa cgtaacgacg ctgcccaaag atatacaaat caagccgttt tacgaccgca
     3301 cccaactcat ccacgaaaca gtgatgacgg ttgtggagaa taccgccgtg ggtattcttc
     3361 tcgtcattgc gacgttggca attttcttaa aagacatccg catggccctc atcgtcgcgg
     3421 tcacgatacc aatgtctctc cttttcgcgt tgcttctcat gtatttttgg aatatcccta
     3481 tcagtctttt atcggtgggg gcgattgact ttggtatcgt tgtcgacggc gccgttatcg
     3541 tcgctgaaaa tatcattcga cggatggggc atagaacaca tttttcagaa cgggagtttg
     3601 gtgaacacat ttatgaatcg gccgtcgaag tacaaaagcc tatgctctac tcgatgctgc
     3661 tcgtgattat cgcatacata ccgctccttt cgcttagtag catcgaagga ttgctgtttc
     3721 gtccgatggc aatcacgcta tgctttgcac ttttgggcgc gctcctcttc gcgctttttt
     3781 tagtaccttc gctcaccaat tatttttacc gtaaaaaatc caacattgct gaaaaacccg
     3841 agggagcgct tttttcgcgc tttaaagaat tttatgcacg aacgcttccc gtgcttattc
     3901 aaaaaagaaa gcgtattgtc gttatttttc tttctctttt tttggtcatt atttctgtaa
     3961 ttgtgcccaa cctcggtact gaatttttac cctatctcga cgaaggtaat ttttgggtgc
     4021 gcgccaattt tcctgaggga agctcgctga gcgagacatc cgaatttgcc gctgagatcc
     4081 gcacgctggt gcgcgaattc caagaggtgt cgtttgtaag ctcgcaaacc gggcgcagcg
     4141 atgccggcaa cgatcctttc ccgatgaacc gcatcgagtt catggttggg gtgaagccgc
     4201 gctctgcatg gaaaaattat aaaaccaaac tggatttaga agcggcgata cgcaccaaat
     4261 tacaaaagcg attccccacg ttgcgtttga atatcacaca accgattatc gacagcgtca
     4321 ccgaggacac gaatggcacc tcggcgaatt tggcaatcga catcatgggt ggcgacatgg
     4381 ataccttgcg acaaatcgct aaaaacacag ttgcgctttt acgaaaaatt cccggaaacg
     4441 aaaacgtgaa tatcgagcag gaagcgccgc aaccgcaact ccagattaaa atcgataaag
     4501 aaaaattagc gcgttaccgt ttgtcggcac aaacggtgaa caccgtcgtg aataccgcca
     4561 tcggcggttt gccggtgagt gaaatttacg aaggtgaacg gcgttttaac attctagtaa
     4621 aatttagacc gtcgcagatg aaatcccctc gtgacattag cctactgccc gtttttaacg
     4681 accacggtga agcggtaccc ttgggtcaag tcgcggcgat tcgagtgatc gacggcgaaa
     4741 cgctgattgc acgcgcaaaa aatatgcgtc gcctcaccgt ccgctgcgac atacgcaacc
     4801 gttcgcaagg cgattttgtc gccgacgcgc aacaccggtt caaaaaagaa atccaaattc
     4861 cggcgggcta ttcggtcgaa tggttgggca tgtttgagaa tttagcgcgc gcaagaaacc
     4921 acttcgcgct ccttattcca tttaccgttg tgttgatatt tcttttgatg ctcgccattt
     4981 ttcgttcggt aggtaaagcc gcgctcgtga ttatgacggt accgtttgcg atgacgggca
     5041 gcatcgttgc gatggcggtg cgcggtatgc acttcagcgt ttctgccgcc gtcggtatga
     5101 cgacgctttt cggtatcgcg acgatgcacg ccattttgat gctgagttac atcgcgcagt
     5161 tggaagcgga aggacggccg cgtctcgaag cgattatcga aggcgcaaag ttgcgtttgc
     5221 gccccgtgct catgacggcg gtcgtcgcct ttttggggtt gttgcccgcg tcgcttgcca
     5281 ccggcatcgg ctcggatgtg caacggccta tcgccacggt gatggtttgg ggaatttttt
     5341 catcggcgtt tttgacgttg tttttattac ccgtcttcta tgctcttttt tttccgatag
     5401 atattgttac gcccgaggtt atgccggtgt cagaggaaat cccaacaaaa gcaaaattca
     5461 atggtcggcg caaagtctca aaaaaactgg gttcatgaaa ctttatggaa gcatcacttc
     5521 gccgtttgta cgccgtctgc gttttgtgtg caacgagctg ggtgtaaatt ttacttttgt
     5581 cgacaccgcc accgatgcgg gccaggctga aatgcgcact aaaaacccga tttggaaagt
     5641 accctgtgtt gaagtcgacg gacaaatttt gtgggatagc cacaccatca tcgaatacat
     5701 ttccgaaaaa aactcggggc accccaaact gttacgcacc ccgcaaggcg cagaaaagtg
     5761 gcgtgaaaag aatttagttg ccgctgccga tagttgcgtt gagtcggcga taaacgtctt
     5821 ttatttgaac aaagacggcg ttaaagtgaa cgaagtgccg tatatggtga aacaaaaggc
     5881 gcgtgtggca tccatcctcg catggctaaa aacccaactt cacgacaatt atttcaccgc
     5941 agagaaaaaa atcggacttt ccgaactcac gctttactgc attctcgact ggatgcgttt
     6001 tcgtgagatg taccctgtga acgacgacgc ggcgctgaag gcataccttg cgttccacgc
     6061 aaaccacccc agcatcgctc aaacaaaatt gccggggtaa ttcaaataca ccccccgcac
     6121 acgccgataa tttaaccact gtgcgtcatc tcaaactagg gtgttattta ttttttttaa
     6181 gcacggcgct ttttgccgat tcggggagtg aaatcgaccc ggtgcataac gtgcttatgc
     6241 atattggttt ggcaattttt gcatccgcgg ttcttgcgta ttttgctttc ctcgctaagc
     6301 aaccggtgat tttggcgtac cttgccgccg gtgtgctctt gggccccaac tttgggttgg
     6361 gactcatcgc gcgcgaaaaa gacatcgcga ctatttctga aatcggtcta attctgctcc
     6421 tcttcatgat cggtatggaa atcgatctga agaaaatcaa agcgtcggga aaatcgctca
     6481 tcacgtcggg agtgacacag ttcgccatca gcgttgcatt ggggttggga ttttttgcgt
     6541 tactgggtta ttcattcagt ggaagcaaat acgacctctt ttatttagcg gtgtgctgcg
     6601 gcatttcttc gaccgccatt gtggtgaagt tgctttattc caagtttgag ctcgatacgc
     6661 tatcggggcg tttgacgctc ggcattcttg tctttcaaga tatttgggcg attatcgttt
     6721 tgggtgtgca acccaattta gcgaacccgc aaattctcga cattcttttg acgtttgcca
     6781 aggcgggcgc gttggtggcg gccagcgtcg cgatgagtaa gtatattcta ccgtatgcat
     6841 tttcgcgcat cgccaaaatc cccgagctcg tgcttttaac gtcgttgggg tggtgcttgg
     6901 cggtgtgcgc ggcggcgttg gcgctggggc tttctctcga gatgggtgcg ctcatcgccg
     6961 gtgtttcgat ttcaacgttt ccttacaacc tcgacgtgat tgcaaaaatc accacgatgc
     7021 gcgatttttt tattacgata ttttttgttt cgttggggat gaaaatcccc cacccgattg
     7081 aaaatttatc gctcgtgggg atggcgttca tcgtggcgct ttttctcatc gtgtcgcgtt
     7141 ttttgtcgat ttaccccgtg ctcttttctc tcaagaacgg caacagggtg agtattttaa
     7201 cttcgcttag tttggcaaac atgagtgagt tttcgttggt gattgcggcg ttgggattgg
     7261 cctcggggca tatcgaccaa aatttactct cgattattat tttcacgttt gttatcacgt
     7321 cgattttagc gccgtacatg attaagtaca accaccagat acagcttttt ctgacgcgct
     7381 atttggaaaa attaggtttc cgcgacagag caaaatttga agagccgccc gcatcgacgc
     7441 tcgacaaaga aattgcaatc ttgggttttt ttcgcgtcgc cagttcgttg gtgttggata
     7501 tacacaatga aaaaagtaaa gattcgttaa aagataaaat tgtcgttgtc gattttaatc
     7561 aagcggtgca tgaaaaattg cacgaatacg gagtggctgc ggtgtacggt gacattgcgc
     7621 acgtcgatac gctgcaccac attggactcc acgacaccaa aattgccgtt tcgacaatcc
     7681 ccgacaatat tttagtcggc acgagcaaca cgaaattagt aaaacaaatc caagacattg
     7741 cgccgcacgc caaaatcgtg gttacggcgg aaaatccgtc gtcggcactc aagatgtacg
     7801 aagcgggtgc cgactatgta tttttgccgc gcatggtcgc ctcgcaacat ttgatgccca
     7861 ttttgcgcga catgctcgcc gctcaggaca gcgaagataa atcaaagacg gcggtggcga
     7921 aacgtaaaaa agccatcgaa cgattaacaa aactcaaaaa agcagaaatg gattttttac
     7981 gccagcggcc agaagtgatt aaataaaaaa attgatgcac ggttacctat gattattcgt
     8041 ggcgataacg ttgcaaaaaa atattttgac gcgctgcttt taatcgtcac tctctttgcg
     8101 gccatcgaag tgccgcttca tttggcgctg gcgtatgaga cgccgcactg ggtacatatc
     8161 atcaattttg tctaccccat tcttttcggt tcggacatta ttctcacatt tttcactacg
     8221 attaccgtcg acgggcaaga gataacttca aagcgcacga ttgcgctcaa gtacttgcgg
     8281 tcgtggttta tcgtcgacct tcttgcggct gtgcccttcg atttaatttt tacacagggt
     8341 tggccttccg aggcgagcaa tatggcgcgc tcgttgcgct tgttttcgcc gcgctatatc
     8401 aacattttgc tgcaagtgcg catgctgcgg ctttaccaca tttttccttt tttggaaaaa
     8461 accacaaaaa aagaattgtt caatcccagc attgtgcgtt tgatattcac cgttttcatc
     8521 gtgcttatcg tcgcgcactg gatttcgtgc gggtggcttg cgttgggaaa agtcgacgca
     8581 acgctggata cattcactaa ttatattaaa gccctctact ggacgatgac gaccatcacg
     8641 acaaccggct acggcgatat tgtgccgacg acaaacgctc aatcggcgta caccattttt
     8701 gtgcagcttt tgggcgccgg tatgtacggt tacatcatcg gtaacttggc gagcatgctc
     8761 gccaacagcg atttagcgcg gacccagttc cgcgccaaaa tggaaaaaat acagaccttc
     8821 atggaatacc gcgaagtacc ggacgagctc caagagagca tccgcactta ttacgattat
     8881 ctgtggaata accgccgtgg ttttgacgaa tcgtcggtgt tggacgaact gcctgcgtcg
     8941 cttaaattgc aagtagcgat gcacttaaac cgcgacatta tcgaaaaagt accgatgttt
     9001 agcaatgcgc ctcaagaact catccaagaa atcgtggtga gtttgaaacc gatgctttac
     9061 acacccggtg attatatttg ccgcaaagga gaaatgggcg atgagatgta tttcatcagc
     9121 cgaggccgtg tggaaattgt ctctgaggac ggcgcgacgg tgtacgcaac gctttcggac
     9181 ggcagcttct ttggtgaaat tgcactcctc tttagtagtg agcgtaccgc gagtgtgcgc
     9241 gccgccgatt actgcgatgt gtacaccttg gataaattta cattcgatgc gatgttggcc
     9301 aaattccccg atgtcgcacg acaagtccaa gtgatggctg aagagcgcaa gcagcgcgcc
     9361 gaaacagaaa atcgaaataa agcatccgtc gatgcatcca cagctatcgt gcgtgaattg
     9421 atagcgagag tttcacaacg ttatgttttt ctcgattggc acacagtcgc tggtgcggat
     9481 ggttaccaag tcgaacgctg ggacgacgca gcgggtggct ggaatttttt atcgaagtcg
     9541 gtttcaatta gcgagtttta cgatgtcatt ccactaaaaa cgaccaatac ttaccgcgtg
     9601 cgcgcaatcc tcggcactga atttgggccg tggtcgcacg ctgtgcatat cgatgtttag
     9661 tacgtttctg ctttttcgcg gcgtttttta atgaagtact taacaacaaa gtacaggata
     9721 cctaaaccga tgaggattaa aataccgttt tcgacgcttc ggatgacttc taccgcgtgg
     9781 tggatttggt cgccaaaaaa ataacacgaa tacacaatcg ctggcaccga aatgagcgcg
     9841 gctaaaccgt cgtagagaaa aaatacgcga aagggtattc ctaacgcccc cgacgaaaga
     9901 aaaactaacg atcgcacacc gggcataaat cgtgccgaaa aaataacttt gttcccgtgg
     9961 tgctccaagc ggtgctttac caactcgatg cgatcggtgt gcataacttt ggccatccac
    10021 ttccattcaa agacacggta gccataacgc ttgccaatgt ggaacattgt accgtcgccg
    10081 agcatcacac ccactaaacc tacaacaatc atcacgtaca cgtcagcttt accgtaatac
    10141 gccatgagtc cacccgtaat tagagtgatg tcttcgggga tgggtaatcc aaaaccacat
    10201 aaaagaagta ctgcaaacac aaggagataa ctatatggtt cgtggatcga ttgaatccag
    10261 ccgacgagcg catcgacgtt aaacatgcta agggcgttcg ggcttttggg aagccgcgtc
    10321 aatgtattat taagttaaga atgacaaatg tttatgtcgc atctatagct gctttttcct
    10381 catgctaaca ggggtaatac accgaaaata gaatgtggac ttaagcgtac gtgtatatga
    10441 aaaagaaggc gagtttgtcg catcctgccc cgagctcgat gtgtatacgt atggcgtcga
    10501 cgccgaggaa gcgcgccgtc gcttaaagca cgttatcgct ttttatgcgt ccaccgctca
    10561 ggacttgggc tataaaatcg acgcatcgga actcttgtct aacctcaagg cgcccgcgca
    10621 cacacgtagc gagtcgtttt tgaattaaag cgaatatcaa tttacaccaa gtaaccgttc
    10681 tgtcgtataa attccataat gcccgaggct ttaccacccc gtattctaat ttttgacgat
    10741 tcagaggtgg agcgtcttta tcttgaggca ttgctcaaaa atgaattcca cgtcgaaacg
    10801 gctaaagacc tcatcgactt ttgggcgcac attgccgaag atgccccaga cctcatttta
    10861 ctcgacgttt atttacccca aatttcgggg tttgaacttt gtaagcagtt gaaaaaaaat
    10921 tctgaatacg catctattcc cattattttt gtaacatcgc tggataacgc caacgacatt
    10981 gaagaaggtt ttgacgtggg tgcccacgat tatgtgacga agccggtgat gcagcgtgaa
    11041 ttaaaagcgc gcatccgagc ggcgttgcgc atcaaacatt tagagcaaga gctgcgcttg
    11101 cgctcggtaa ccgactattt gacgggcccc tataaccgcc ggtattttta cgaagtcgtg
    11161 atagcaaaca tgagctacgc cattcgcatg cggcgcaatt tgtgtatcgc catgctcgac
    11221 atcgattttt ttaaaaaagt aaacgataac tatggccacg aagcgggcga tgccgtgctc
    11281 aaacatttca ccggtacaat aaaataccag atccgcaaat acgatatttt ggcgcggtat
    11341 ggcggcgaag aatttgtgat tcaatttttc gattgtacgc ttgagcgcgg acttgagttc
    11401 ttaaatcgta tccgcgatgc gctggcaact tcgccttgcg tgtacgaatc gcacaccatt
    11461 tactatactt ttagcgcagg tattgtgtct ttagaagaag tcgatgctga ttctccgctt
    11521 gagcgcatga ttgaaatggc cgacaaacgt ctttatagcg caaagaaatc tggtagaaac
    11581 cgtaacgtct caatagacgt ttgagttttc agcatatcgc ctgtttcaac ttaatcattc
    11641 aatgcgcgat ttaatccgtt tagcaaaagg tggcatcatt ttcaccgtcg taatcactgg
    11701 ctttacggga atgctcatgg caaagcgctg ggtgccgccc gtgtgggatg ttctttgggt
    11761 ggtggcgtcg ctcaccctct cgtcgggtgc atcggcgctc atgaacaatc ttttcgatta
    11821 cgacctcgat aaaaacatga agcgcactct ctggcgttcg gggctcatcg atcgtttcgg
    11881 caaacgcaac ctttggatac tcgcagcggt ggcgtcggtg ctgtcgttgg tgccgttgat
    11941 tgttttccag cggtggtggg ctctattttt taccgtcgct gcaatttttt catacgcggt
    12001 ttggtacacc atttacttga aacggcgcgg gcctttcggc gcgattgtcg gcggtttgcc
    12061 gggcgcgttg ccggttctca tcggtgctta tgcagtgtcg gatcgtttcg cgcccgatgt
    12121 gtggcttctc ttcgcgttta tgatgctctg gcaacctgca cacttttggg cgctcgcgct
    12181 aaaaattcaa ggcgagtacg ccaaaggtgg cattccggtg ttgccgcttg tctacggcaa
    12241 cgagtacacc cgcctctata ttttgcttta tggattttcg ttaccgccgt tagcgttggg
    12301 agttggtctg tcgggggggt atagcctttt ttatctcatt gccagttctg tcgcttcagt
    12361 gcactttctt taccgcacta tccgtggcat ttatccccaa acgaacaaag acgcgaacgg
    12421 cgaagtcgct ccgcctcaat atggtcgcgc tttttttgcg tcgattattt acatgatggt
    12481 ggtgatgttg ctcgtgattg ccgatatttt tttatcgcca ctttttcact aagatttgct
    12541 ttttttctta cttaattttt gggcaggagc tttctttttt ctcgcgctgt ttgtagcgcg
    12601 agaatatctt tctttcgttt gcatcgttgc cggttttagc gcaggcactt cgtcgaccga
    12661 atcattttgt tctttatcaa aaaaatggga aaaataattt tttatttttc ttccatataa
    12721 ttttacgctc acccacgccg ttagcgcgct agccaaaaaa acttgtagcg caagactacc
    12781 cgtgcctggg tcggtgtatg cataaagggg atgaaccaaa aaagcgataa aaaaaagaga
    12841 acagagttta cgcagcatgt aaaaaaaaca aagaatactt tgcattcatg ctctcattgt
    12901 caattccttc tttaattatc tttattgcct cacttttggc gtttgtcttt ttggaaggat
    12961 tattcttttt tacacgccct tcgtttatct cgttgacaga accagcaacg gtgttctctg
    13021 ccgtttttgt agcgagtttc tttgtcgtgt ttatcggcgt ttttctctgg ggattttttt
    13081 ggggtctaga atttctactg cgccgctatt tggactccca cttacggcta acgctccttc
    13141 cctcggcgtt tgtattggcg ctgcttgcat tattgctctt ggacaatttt acttacacag
    13201 tttttcatat cggtattata tccaccgata tactgggacg actcgtctac gttggccttt
    13261 tttcatacac tattttttac ttgtttcatc gcctcacttt cttcgtccat gaaaaaaaag
    13321 aactattaag cagcaaccat gcactcttaa cgttagctct cccacttggt cttgcattta
    13381 tttttttcat gagcaaggca gcagtacccc tcaaagcgtc gctacttgag acaggggtac
    13441 tcacaaaaag gacacgccct aacattattt tttttgcttc cgatggggtg gacgcccagc
    13501 acttaaagct atacggatat gcacgcaaca caacgcccac tctcaatcgc tttgcgcacg
    13561 atcgtaaacc gcttctttgc aaaaacgcat tttccaacgg cgcacgcacc accggttcct
    13621 taacggcgat gctcacaggg gtctctcccg cgaccaataa agtatttttt cccccgcata
    13681 ttttaaacgc aaccaatgcg caccgccatt taccgggcat acttaaacag ttgggatata
    13741 tcaattttca agaaacaatt cgatattacg ccgacgcggg cgatttgaat ttgcaaggtg
    13801 cgttcgatgt cgcaaacaat cgcagtctta aaggacagaa ttttctggga cgccgattcg
    13861 tgactgaaag ttattttctt gccgcacttt tcgagcgctt atcgagccgc cttctgcata
    13921 tcaccggcgt gcgttccatg gaaaattttg ccgcacagat gaacaatgaa gacagcccca
    13981 acgaagtgta tggcacaatc gatgacacgc gcatggaacg ccttgaaggt ttcatcgagc
    14041 aggtgaaaga acctttcttc gcacacgtcc atttactcga ctcgcattgc tgcacgttca
    14101 agtttaagca cgcacacttt tcacgccgta ccgcagagga aaaagaaaca aaaactcaac
    14161 ctctcgaacg cgatttaatc gacgacgcta ttttagcaag cgacgcacag tttgcgcgtg
    14221 tgctgaaagt ccttaaagaa cgcggtattc tagagcgtac tatcgtggtt tatagctccg
    14281 accataaccg cggttggaca ataaaacgaa aagtcccact ggtgttcttt tttcccgata
    14341 gccactatgc gcgcatagaa aaaaagaatt gtcagctttt agatgtcgct cctactttac
    14401 tcgattattt agatattaaa acaccgcatt ggttggaagg acgctccctt ttggacgaag
    14461 caaatctcga taacgctgat agaatttttg gttttttgaa tatacgccgc cactcgattc
    14521 aagtcggtaa atcaaaagta agcgagcttg tcgatgccgg tgcgccgtat tatggattga
    14581 cttcgtatgg aatgatgagc tgtaataagt ggtatgagct caacttaaac aacggcaaaa
    14641 tgaagtccgg taagatttca gggtactctg gggggtgcga aaaaggaatt ttacccactg
    14701 agctaaacgc aaaaaaagaa attgtgcagt atttaagttc aaaaaaattg ggaatgcatt
    14761 ttcagaaaac gagcgcgaag tagtcaagtt gtttgattta tcttgcgctt ttatagaaat
    14821 gagttgattt tacttgggac cgaccatttg gtacaaagac acgtgaggtc agtcccatag
    14881 attatgtaaa gacgttctcg aagttatttt gcttcgacaa cagagaaagt aacgcgacgg
    14941 ttattttctt tagaaaggtt ggggtcttgt tgtgaatcac ccgcaccgac ggtcactaat
    15001 ttgttcgcgg cgagaccttt ttttactaag tagtttttta cgaacacagc acgctgtgtt
    15061 gaaatgtttt ttacaaacgc cgccgaacga ccactgtttg cgttagcgtg gccggtgagt
    15121 tggattttgt aaccttcggg caatgttgcc acaacgcctt tagcgacagt tgccgcattt
    15181 tcaccgtatt tatcaaattg gctcatgggc atcgacgact gataaggagg aaaaccaaca
    15241 acaggaattt tagcgagttg ttcgttcgcg gcatcaatac cgaaaccgct gctgacagaa
    15301 cggacttctt ctgttttttg cgttgaaccg caagccatag taaaaccaac aagagcaata
    15361 agtgcaattg caaaaaattt atttaatttc atttgacctc tcaaaaattt atttgtacac
    15421 taacggggtt tcgcgatttc cagtctgccc acacatgtac actaacataa ataaaattta
    15481 agacatgcgg tcaaacagct ctttagttgc gagttctcaa agtcatagac gctcagtcta
    15541 aaaaaataat tgtaaatgag cgatgagcga ctggaaaaat gaaatcctca aaaatttgaa
    15601 cgagccgcaa cagcacgcag tgcaaaccac acaaggaccg cttttaattt tagccggtgc
    15661 ggggtcgggg aaaacgcgca ccatcatcca ccgcatggcg tatctcatcc acgttgaaaa
    15721 agtgcctgcg tataaactcg ctgctgttac gttcaccaac aaagcggcga atgaaatgcg
    15781 cgaacgtttg ctcaacgtcg caggccccat cggcagtgac tcaaccgtgc gcaccttcca
    15841 ctcgttgggg ctttttcttt tacgccgcaa cgccgccgcg ctcgaatacc ccgaaaattt
    15901 ttcgatatgg gatgacgccg atcaacaatc ggcgctttcg caaattttag aaaagtttga
    15961 gggtaagttc aaccgcactg aaactcgcta ttttgcaaac agcatttctt cttttaaaga
    16021 ccgtttagtg acaccgcaaa atttagtcga cgaggtggat ttagacgcgt acgagttcgg
    16081 cgatatttta caagaagtgt atcagcttta cgaactaaaa aagaaggcgt cgtttgccgt
    16141 cgactttgcc gatttaattt cactgccgtg ttttcttttc gataaaaatc ccgatctttt
    16201 aacgcactgg caaaaccgct atccctattg gctcatcgac gaatatcaag acactaattt
    16261 tgctcaatac cgttttgtgc agcacgttgc cgcacgcgac agaaatttgt gtgtggtggg
    16321 cgacgacgac caagcaattt atggctggcg tggggcggat gtcaaaaata ttctcgattt
    16381 ttctttggat ttcaaagacg ctaccgtcgt taaacttgaa gaaaactacc gctcgacaaa
    16441 accgatttta gacgtagcaa atgccgtcat tcaaaacaat ttagagcgca tggagaaaac
    16501 actctttacg aatcgcgcag gtggcacacc accactcttg atttcaacga gcgacgatgc
    16561 ggcagaagcg cggcaaattg ccgcgctcat ccaaaaagcg caacaagcgg gagttcccaa
    16621 ctcagagatt gccgtgctct accgcaccaa cgcacagtcg cgtctcatcg aagaagcgtg
    16681 cctcaacgcc aaaattccct accgcgttta cggtgggctt tcgttttttg cgcgtaaaga
    16741 aattaaagac atactggcat acttccgcct cattgtcaat ccacacgacg agacggcgtt
    16801 cttgcgcgtc atcaacaccc cctcgcgcgg catcggcgaa aaat
//
LOCUS       DAOMPD010000005        16727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1509_length_16727_cov_2.926823, whole genome shotgun sequence.
ACCESSION   DAOMPD010000005 DAOMPD010000000
VERSION     DAOMPD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_16727_cov_2.926823"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLY93_00405"
     CDS             complement(<1..385)
                     /locus_tag="PLY93_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67981.1"
                     /translation="MAQSIRQFHLRQRLDRRLTGLTIFTIAMLGILFLRIGDLQLVRG
                     DDFFEKSQRVIRRVVALSAPRGEMFARNYTSRDKATYIVSNSSAVSLSVIPSHFPKKA
                     ELKESVAKLERLLRYSEGSLTVKVAD"
     gene            complement(391..945)
                     /gene="mreD"
                     /locus_tag="PLY93_00410"
     CDS             complement(391..945)
                     /gene="mreD"
                     /locus_tag="PLY93_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRP67982.1"
                     /translation="MILEKVVIGIAIFVSYFLQTSVDFFALGSIKPDFILILTVYFAL
                     YRGPFAALWIGFWGGILQDINLGGYTNLVQQDMRYYIGTSALAKTIVGFVTGKIAHNI
                     NKDATSLIVIVVFLACLAKGVIMFFTVAFFHSSAHASALITIVIPEALFTAILSIVWF
                     RLLRWAFPTIEKTEGQSIIRRMGR"
     gene            complement(942..2036)
                     /gene="mreC"
                     /locus_tag="PLY93_00415"
     CDS             complement(942..2036)
                     /gene="mreC"
                     /locus_tag="PLY93_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRP67983.1"
                     /translation="MLADILYRYKAASSLVFTVVFSLSCLIWKSNFLSRTANSASRTL
                     DFFSATFSSFGKGVSRFVDSYGTYENVRNERDALREKVKQNLDMQIELVRLREENNKL
                     RQFLQLPPVSSYPVLQAEVISQDPDNWFRTIIINKGSVDGVAAYMPVVALQTVTQPAT
                     GDGKSEANLVVGVVGKVIQVNPHSARILPLTDQFSRIGVTVKRTGHWALLNGQSPQQD
                     LPLLDYLSLGVFISTGDEIVTSGGDGIFPRGLPIGRIGKKIERLGSFQKAEINPAIDF
                     KKLDFVIVIQKKVETEALNFLPLRPDTVEEPKLPPPVQAPAKPNPGAATERLKELASP
                     NANPQKAEPKNDGAPKKEPVEDADDEDAAP"
     gene            2152..2877
                     /locus_tag="PLY93_00420"
     CDS             2152..2877
                     /locus_tag="PLY93_00420"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRP67984.1"
                     /translation="MCLIVLSYQPTADFPLVVAANRDEFYARPSAPLSWWHDEPTILA
                     GRDLKEGGTWMGVTIQGSFAAVTNFRTAEDVNLLSLSAKHLSRGALVRDFLQSGNTLE
                     FSKYLVANATRYKGFNLFFGNINHLYYFSNHSQKGVEELTPGVYGLSNHLLNTPWYKV
                     TQAKKMFQQTDPSDSEAVFSLLKNDTPAPDDQVQQTGFPFVMEKMLSSIFIKAPGYGT
                     RVSSHLCLSKNEIRFAERTYSPT"
     gene            2912..4726
                     /gene="recQ"
                     /locus_tag="PLY93_00425"
     CDS             2912..4726
                     /gene="recQ"
                     /locus_tag="PLY93_00425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRP67985.1"
                     /translation="MISGTTTIKDARHILKHVFGYETFRFEQERIIESVLKKEDTLVV
                     MPTGGGKSLCYQIPALLFEGLTIVVSPLISLMQDQVSQLMQLGIPAVCLNSALDLMEY
                     EENSTQIRTGKARMLYLAPETLFLSRTQTLLREVRADCLTIDEAHCISDWGHDFRPEY
                     RQLAEMRKKFSGAVTVALTATATAQVRHDIKEQLAITDSHEFIASFDRKNLSLHVMPK
                     SAPESQVLSILREHPNESGIIYCFSKAQVDDLAHMLYSRGFSVKPYHAGLADSLRAKN
                     QELFIRDDVQIIVATIAFGMGINKPNVRFVIHYNLPKNIESYYQEIGRAGRDGIKADC
                     FLLYAHSDSQKIKFFIAQKEEKEKRTALRQLATLVDYAESDCCRRQTLLAYFGEASPE
                     YCGACDVCVNPPKEKIDLTVAAQKLMSCVKRSGERFGATYIVDVLRGSRLEKILANGH
                     DKLLTYNIGKEFSKKGWMSLVRQLVQQGLLTQDAEFGSLMLTADAVAVLKNEKVFMGN
                     PLESAVPTFYRERGTDDAVEKNGELFEILRKRRKELADEQNLAPYMIFSDKSLIDMCV
                     RLPQNRDEFLKVHGVGETKMRRYADEFLPYLRACAPTR"
     gene            4811..5786
                     /locus_tag="PLY93_00430"
                     /pseudo
     CDS             4811..5786
                     /locus_tag="PLY93_00430"
                     /inference="COORDINATES: protein motif:HMM:NF027965.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
     gene            complement(5859..6896)
                     /locus_tag="PLY93_00435"
     CDS             complement(5859..6896)
                     /locus_tag="PLY93_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67986.1"
                     /translation="MSIQLEKTYATANFTRFKGVASGGHYESYFMRANDAATGRAFWI
                     RYTIFQSEKNANTKAPMGELWAVYFDKGKMPIAAKSEIPLSAISYSNTNFNLNFDGAT
                     LNDYALKGSAGNLGPNRIEWNMSLQRMPDAKNTNPVLPLPLSAYDLPVPRAKLLVSQP
                     FVMFKGTLTINGKTIAIADWQGSQNHNWGSRHTDEYAWGQVAGFDGAPDSFLEVASAK
                     MQLGFFTTPYLTPIVVRHEGKEYALNGYWQSIRNKATVDYFDWKFWGENDALKIEGHI
                     HADGKDVVALRYYNPPGDSKACLNTKVATCSLAVQEKGRLGLTFELTTKNRAAFEILT
                     SKDNHGLLPLF"
     gene            7098..7463
                     /locus_tag="PLY93_00440"
     CDS             7098..7463
                     /locus_tag="PLY93_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67987.1"
                     /translation="MKKIVIVLVGLFLVSSLSAVDDALAIARREIGVQFDLLKKGKTT
                     QLKTHFTDRQKARVVDMRVKRAQKETAQYKITDLVHSAQEISHNGRRTIKVKMRNERT
                     LTTLVEIDGKWYADTVWFK"
     gene            complement(7555..7626)
                     /locus_tag="PLY93_00445"
     tRNA            complement(7555..7626)
                     /locus_tag="PLY93_00445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7591..7593),aa:Arg,seq:ccg)
     gene            7765..7929
                     /locus_tag="PLY93_00450"
     CDS             7765..7929
                     /locus_tag="PLY93_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67988.1"
                     /translation="MKKLVMILAAVSFLAGFSVAAKTNTQVKMEARAKAQKEKSAQKR
                     AANKAKSKKR"
     gene            8320..9459
                     /locus_tag="PLY93_00455"
     CDS             8320..9459
                     /locus_tag="PLY93_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67989.1"
                     /translation="MLLQTMAANLALYSLRRFVRLQNLVIACAALAFILFPFLLALLI
                     GFDTGLFFYVLAFLPIGFLAFPNNIFRAATTLAINIAFLVAMHVLALKWKPHYPAPDY
                     IKIELNAIVIVLSILLFGIQILYLGFLTQVFQRIREVWMQYAQLGAEKYTDKNRKRNH
                     IISNQIIMVFTIILVVMAVAQAVLSIILLVRQFETYIYYSLYYLVPTLLCIFLFLQIF
                     HLKVKSNTNTRFEAIIFTTGNLYTLYLAYIQGREIGLHYVFLPLILVPTFFSIASARF
                     KYAMTILPITFLDSVFSRCKTAGIPYPTTLLNLSVKYSRLVISLFFSLLSFIFGLNRD
                     LKSDFSLFGASGVCAVCISLIRKLIENTALCKTNYYWCLSSSRHS"
     gene            9456..9875
                     /locus_tag="PLY93_00460"
     CDS             9456..9875
                     /locus_tag="PLY93_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67990.1"
                     /translation="MIINEIKILVSLIQLGFNDTLGFILHYVVPTFFSLLLLTACFYA
                     NQFTRSFVPISIAYLWGILLLGYVGISVQADSFFYIFALALLPLPFIALSGKTPWEKM
                     LLGLGFSALALIFFSRIDFCNGTHRFSLCPMTRLSRS"
     gene            9887..10531
                     /locus_tag="PLY93_00465"
     CDS             9887..10531
                     /locus_tag="PLY93_00465"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP67991.1"
                     /translation="MATLIATFIVYFFYTTAQARLSESEIETERMKSDQLLLNILPID
                     IARELKDKGATEPILFHSATVFFTDFVGFTKITETMTPHELVGELDKCFSYFDNVCEH
                     YRLEKLKTIGDSFMAAGGIPIPNNTHAIDCCLAAMEIRDFMDQMQQIKEQQNQPYWQL
                     RIGINTGNLVAGVAGEKKIRIRCVGRHGEHCQPHGIGRCSWRNQHLRSNVSTSA"
     gene            10467..10691
                     /locus_tag="PLY93_00470"
     CDS             10467..10691
                     /locus_tag="PLY93_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP67992.1"
                     /translation="MESAGVPGEINISEATYLQVRKFFVCKTRGKISAKNKGKITMYF
                     LHGIKTEYSVDGKGRVPNERFKEIIAAERL"
     gene            complement(10669..11112)
                     /locus_tag="PLY93_00475"
     CDS             complement(10669..11112)
                     /locus_tag="PLY93_00475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRP67993.1"
                     /translation="MLTFHKARRTNRADLLRLDQIIFREAAYSVESWHQELLNPASHV
                     YLAYEENEPVGFISILLSGGDAEVVKVGVVPSFRRRKVASGLINHALHALAAERCLID
                     VSEKNTAALLFYQKLRFVDLARRRSYYNDGSDAVVMELVKVARPQ"
     gene            complement(11113..11487)
                     /locus_tag="PLY93_00480"
     CDS             complement(11113..11487)
                     /locus_tag="PLY93_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRP67994.1"
                     /translation="MLLIKLIIIFAAFCFLLYALSSAISDFQISNGAIPIVALILVGV
                     NYAVTWIFTALNFITLGIVKWLLMVLTLGLFYLLAGFILNVIIFWIADRFTDKLTIKS
                     KKALFISAFALQITTYIIDKIF"
     gene            11590..12150
                     /locus_tag="PLY93_00485"
     CDS             11590..12150
                     /locus_tag="PLY93_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRP67995.1"
                     /translation="MSKKVNIFLVVGVCFIAFFSSCASIPEKAKPIENFDVNRYLGTW
                     YELARFDFRFERNLDNTSAHYALDKKGNVIVLNSGYNYVEKKWSKAVGLAKFRDAKNV
                     AALKVNFFGPFYSGYNVIALDDNYQYALIAGKNLDYLWILSRTKKLPDEIKAKYLKIA
                     EEIGYDISRLIWVKHDNDNNPFLNEN"
     gene            12140..13453
                     /locus_tag="PLY93_00490"
     CDS             12140..13453
                     /locus_tag="PLY93_00490"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRP67996.1"
                     /translation="MRTKITVFWFRRDLRLKDNKGFFEALSESFPVYPIFIFDKNILT
                     KLEDKHDRRVDYIHQALTNINHELKTVQSTLNTFYGNPLAVFQQLSKDYVIKAVYCNR
                     DYEPSAILRDTEIYNFFKNQNVPFKAFKDQVIFDKNDVLKNDGAPYTVYTPYSKKWKE
                     RLTVLHYKANTYTLKNLYKNKFTEIHSLKDVGFEKTEITFKKPELNVSIISEYDAYRD
                     YPAMGRTTQLGITLRFGTISVRDCVAFAVEHNQTWLNELIWREFFMQILYHFPRVVYQ
                     SFKEKYDYIQWLNNEREFELWRKGKTGYPIVDAGMRELNQTGYMHNRVRMIAASFLCK
                     HLLIDWRWGEAYFATKLNDYDLSANNGNWQWAAGSGCDAAPYFRVFNPTLQTEKFDKN
                     LEYIKKWVPEYGTEEYPKPMIEHSFARERALQMYNQGLDRSKNYE"
     gene            13446..14348
                     /locus_tag="PLY93_00495"
     CDS             13446..14348
                     /locus_tag="PLY93_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRP67997.1"
                     /translation="MSKVLIAGATGFIGRYLIGYLLEKGDSIHALTRRKQNSEQSNIS
                     YFEWDVATGFIDGNAFDKVSSIINLTGANIGDQRWTNKRKTEILESRTKPIQLLYNYV
                     REHSLKIDAFISSSAVGYYGAVTSDEVFIENSVKGADFLSEVCQKWEHAAFQFSALGA
                     RTVVLRKGVVIGKDGGIYQKLAPLAKRSLNTALGDGKQYLPWIDVRDVVRLYEFILTH
                     REVSGVYNAVATEHITMNDFSEALLASFGKKSILPNVPKFMIQIAVGEMSVMLLKGSK
                     VSNQKLRNTGFEFEYEKIKNALDL"
     gene            14516..>16727
                     /locus_tag="PLY93_00500"
     CDS             14516..>16727
                     /locus_tag="PLY93_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP67998.1"
                     /translation="MFLKQIALISASTLFILSCARLGEAKSLTSAQEAVATQLIIPTD
                     ATKPGMGQDGGPLTWSFWVKNRQASAELMLGSFTPFYKINTLRRAVSQHFVLYADTAS
                     AALVSSSVNRIAVLTSGSTTVAGLSATGDLAVGMRVTGGGLPCATYIASITNSAVVEI
                     TNPAPTSGTPTLTFAHGPSVILDSMECAYSNLLHVYGGDKHPYANNNARIVILANDIL
                     DDYATTGGYVGGYFAPRDLYSNAFTTSLFTNPAAIQQNAQLVGQLGGYSNEMSVVYYD
                     LNPGYTTAPNQVNDIVLHELSHLFTYNYRVIQQRYKNLDLWIAEGVAENAPPQTNGSG
                     NLLTLRLSQMGSPALFASYQAAPQLVDFQTWGPKIVGYLQSNLFFSYLRHRLELNSTG
                     SAASFVHDAVTQIDTSMNGINALISTYLPSENFSTIYQDFVTTHYLMLLGIQIEDTNG
                     MNGGGINLQQKYSFCKSEIGISNTGVNNCTSIKARYSSNIPFNYDAPRCSDNTYGLKP
                     NSYIIYRHKVAGDRTHFDIPGTDPVAGELPLKIIINTTPEATLISGPPATVNLYTYNA
                     GDNIPFTSSLGFVNGDIAQIIVVNPNPTGSCRKLDETWLLKRNHSKWVGQNSFGVHAS
                     PDFYWQSDAGASWLTDTDGAYYRPAGLAADLQGYPNNRLYISDYINMSVQRLNLDTGE
                     PLGRLGSPSNTCPTTGMPWSTSTNRYLNGYCAHNFNSPQGLAVHKDHNLYVADTD"
BASE COUNT         4725 a         3810 c         3704 g         4488 t
ORIGIN      
        1 tatccgccac ttttacggtt aaagaaccct cgctataccg taacaatctt tcgagctttg
       61 ccaccgattc tttgagttct gcttttttag ggaagtgaga cggtatcacg cttaaagaca
      121 ccgccgatga attggaaacg atgtacgtcg ctttatcgcg gcttgtatag ttacgcgcaa
      181 acatttcacc acgcggcgca ctcaacgcca caacgcggcg gataacgcgt tgtgactttt
      241 caaaaaaatc atcgccacgt acaagctgta aatcgccgat gcgtaagaaa agaataccca
      301 gcatggcaat cgtaaaaata gttaaaccgg tgaggcgtct gtcgaggcgt tggcgtaaat
      361 gaaattgtcg aatcgattgc gccattgatg ttatctaccc atgcggcgga tgatactttg
      421 accttctgtt ttttcaatcg tggggaaagc ccagcgcaac aaacgaaacc acacaatcga
      481 aaggatggca gtgaagagag cttcgggtat aacgatggtg atgagcgccg atgcatgcgc
      541 gctggagtga aaaaatgcca ccgtaaaaaa cataatcacg ccttttgcaa ggcacgccaa
      601 gaaaacaaca attacgatga gcgaggtggc gtctttgttg atgttgtgtg caattttgcc
      661 ggtgacaaag cccacaatcg tttttgctaa agcggatgta ccaatgtagt aacgcatgtc
      721 ttgttgcaca agattcgtgt acccacccaa attgatgtct tgtaaaatac cgccccaaaa
      781 gccaatccag agcgcggcaa aaggaccacg gtacagcgca aaatatacag ttagaattaa
      841 aataaagtcg ggtttaatcg aacctaaggc aaaaaaatcg acactcgtct gtaaaaaata
      901 tgaaacgaag atggcaatcc caataacgac tttttccaga atcatggtgc ggcatcctca
      961 tcgtcggcat cttcaacggg ctcttttttg ggggcaccgt cattttttgg ttcggctttt
     1021 tgtgggttag cgtttggaga cgccaattct ttaagccttt cggtggcggc accgggatta
     1081 ggtttagccg gcgcttggac aggcggcggg agtttgggtt cttcgacggt gtctggtcta
     1141 agcggtaaaa aatttaacgc ttcggtttcg actttctttt gaatgacaat cacgaaatct
     1201 aattttttga aatcaatcgc cggattgatt tctgcttttt ggaaactacc gagacgttcg
     1261 atttttttcc caatcctccc gatgggaagt ccgcgtggga aaataccatc tccacccgat
     1321 gtaacaattt catcgccggt ggaaataaaa acacccaaac tcaaataatc caaaagcggc
     1381 aaatcttgct gtgggctttg gccgttcaag agagcccagt gaccggtgcg cttcactgtg
     1441 acaccgatgc gcgaaaattg atcggtgagt ggtagaatac gcgccgaatg tggattgact
     1501 tggatcactt tgccaacgac accgacgact aaattcgctt cgcttttgcc gtcgcccgta
     1561 gccggctgcg tcaccgtttg caacgcaacc accggcatgt atgccgctac gccgtcgaca
     1621 ctccccttgt tgatgatgat ggtcctaaac cagttgtcgg gatcttgtga aataacctcg
     1681 gcctgtaaaa cgggataact cgagacgggg ggtaactgta aaaactgccg caacttatta
     1741 ttttcttcgc gcaaacgcac caactcaatt tgcatatcga gattctgctt tactttttcc
     1801 cgcaacgcat cgcgttcatt gcgcacgttt tcatacgtac catagctatc gacaaaacgc
     1861 gaaacccctt taccaaatga cgagaacgtc gccgaaaaaa aatcgagagt acggcttgcg
     1921 ctattggcgg tgcgtgagag aaaattcgat ttccatatta gacagcttaa agaaaaaaca
     1981 acagtaaaca caagcgatga cgctgctttg taccggtaaa gaatgtctgc gagcatggcg
     2041 ggttaaggaa tttaaattat gtctcattat ttaagacgat acgtcaactt tattaagcag
     2101 ttataaaatc aaactttacg gttgatgcac aagttctgaa gttctaaaaa aatgtgtctc
     2161 attgtgctct cgtaccaacc tactgcggac ttccccctcg ttgttgcagc aaatcgtgac
     2221 gaattttatg cgcggcccag tgccccgctt tcatggtggc acgatgaacc caccattttg
     2281 gcggggcgcg acttgaagga aggtggcact tggatggggg taactatcca aggcagcttt
     2341 gcagcggtga caaattttcg tacagcggag gatgtaaacc tcttgtcgtt gtcggcaaaa
     2401 catctttcac gcggcgctct cgtgcgcgac tttttgcaga gcggcaatac tcttgaattt
     2461 tcaaaatatc ttgtagcaaa tgcaacaaga tacaaaggat tcaatctgtt ttttggtaac
     2521 ataaaccatc tttattattt tagcaaccac tctcaaaaag gcgttgaaga attgacgcca
     2581 ggcgtttatg gtctctccaa tcatttatta aatacaccgt ggtacaaagt tactcaagcc
     2641 aaaaaaatgt ttcagcaaac ggatccatcc gacagcgaag ccgtgttttc acttttaaaa
     2701 aacgacactc ccgctcccga cgatcaagtg caacaaaccg gatttccttt tgtcatggaa
     2761 aagatgcttt catcgatatt tattaaagcg ccagggtatg gcacgcgcgt gtcgagccac
     2821 ttatgtcttt ccaaaaacga aatccgcttc gccgaacgga cgtactcacc aacataactt
     2881 tacgagcact ttcacatcga gactctgccg cgtgataagt gggactacca ctatcaaaga
     2941 cgctcgacat attctcaagc atgtctttgg ttatgaaacg tttcgttttg aacaagagcg
     3001 tatcattgaa agtgtgctga aaaaagaaga cacgttggtg gttatgccca ccggtggcgg
     3061 taaatctctt tgctaccaaa tacccgcgct gctttttgaa ggtcttacca tcgtcgtctc
     3121 gccgctcatt tcgctcatgc aagaccaagt atcgcaactc atgcagcttg gcataccggc
     3181 ggtgtgtctc aacagcgcgc tcgacctcat ggagtacgaa gagaattcca cacaaatccg
     3241 cactggaaaa gcgaggatgc tttaccttgc ccctgaaaca ctttttttaa gccgcaccca
     3301 aacgctctta cgcgaggtac gcgccgactg cctcacgatc gacgaagcgc attgtatttc
     3361 agactgggga catgattttc gccccgagta ccgacaactc gccgagatgc gcaaaaaatt
     3421 tagtggtgcg gtaacggttg cgcttactgc aacggcgacc gctcaagtac ggcatgacat
     3481 caaagaacag ttggcgatca ccgactctca cgaatttatc gcaagcttcg accgcaaaaa
     3541 tttatcgttg catgtgatgc ccaagagtgc gcccgaatcg caagtacttt ctattttacg
     3601 cgagcatccc aatgagtcgg ggattatcta ctgcttctcg aaagcgcaag tcgacgattt
     3661 ggcgcacatg ctttattcgc gtggtttttc tgtaaagccc taccacgcgg gtcttgccga
     3721 ttcgttacgc gcaaaaaacc aagaactatt tattcgtgac gacgtgcaga ttatcgttgc
     3781 gacgattgcg tttggcatgg gtatcaataa gcctaacgtg cgctttgtga tccactacaa
     3841 tctccccaaa aatatcgaaa gctactatca agaaattggg cgggcaggac gcgatggcat
     3901 caaggcagat tgttttttgt tgtatgcgca tagcgacagc caaaaaatta aattttttat
     3961 tgcgcaaaaa gaagagaagg aaaagcgcac tgcgctgcgg cagcttgcaa cgcttgtcga
     4021 ttacgccgaa agcgattgtt gccgtcggca aacattgctc gcgtactttg gcgaagcctc
     4081 gcccgagtac tgcggagcat gcgacgtgtg tgtaaatcct cccaaagaaa aaattgacct
     4141 cacggtagct gcccaaaaat tgatgtcgtg cgttaaaaga tcgggcgagc gtttcggcgc
     4201 cacttatatc gtcgacgtgt tgcgaggctc gcggctcgaa aaaattctcg ccaacggaca
     4261 cgacaagctt ttgacgtaca acatcggcaa agagttttca aaaaaagggt ggatgtctct
     4321 cgtacggcaa ttagtgcaac aggggctatt gacgcaagac gccgaatttg gttcgctgat
     4381 gctcaccgcc gatgccgtgg cggttttgaa aaatgaaaaa gtgtttatgg gtaatccgct
     4441 cgaaagcgcc gtacccacct tctaccgcga acggggaacg gacgacgccg tggagaaaaa
     4501 tggcgaactg tttgagatac tgcgtaaacg acgcaaagaa ctggcggacg aacaaaacct
     4561 cgcaccgtac atgatttttt cagataaatc gctcatcgac atgtgcgtac gcttaccgca
     4621 aaaccgcgat gagtttctta aagtacacgg cgtaggcgaa acaaaaatgc gccgttacgc
     4681 cgacgagttt ttaccttact tacgagcctg tgcgccaact cgctgagaca cgctgcgcaa
     4741 gttggcgcac aggcttttaa gcaaaaaata tttacgcgac aaagcctcat ggcattacgt
     4801 aaagtttttt atgctccatt caatccgagt cgccatcgtc ggttacggta acgtaggccg
     4861 tggtgttgca acagccctca cccaaaataa agacttaacg ctcgcagcca ttctgtcgcg
     4921 ctcgcccgaa cgcgtcaaaa aagaaattac aacaacccct gtgcattctg tggacgacac
     4981 cgccattttg aaagacatcg atgtggcgat tttatgcggg ggctctaaag acgatttacc
     5041 ccaacaatgc ccccattttg ctgcgtacgt caacaccgtc gatagtttcg acaaccacag
     5101 caagattccc gaatattttg cgaatatcga caatgtttca cgaaagagtg gacgcgtttc
     5161 aattatctcc gcagggtggg atccaggcac tttttcaatc gaacgcctca tgggcacggt
     5221 agttattccg aacgcaaaga catacggatt ttatggacta tcccctcgtg gtggattgag
     5281 catgggacat tcggatgcat tgcgtacaat ccgtggcatc aaagatgcac ggccccgaat
     5341 cgattgaaaa aattaggaac ggtgaaaatc cgacattgac aaaccgcaat atgcactggc
     5401 gcgaatgttt tgtcgtactc gaagaaggcg cggacaaaga aaaaattagg caagaaattg
     5461 tgtcgatgcc cggttatttt gaaccataca aaaccgtagt gaattttatt tcgcaagaag
     5521 agctcgaccg caactattcg ggcttcccac atgatggtat agttttgggc tcgggaaaac
     5581 ttggcgacgg tactaccgct cttttagaat accgcaacac ttgggcaagc aaccccagcg
     5641 gcacagcgca cattttagtc gctgctgcgc gtgcggcaca ccgtttggcg gtagaaaaaa
     5701 aatcgggggc gttcacgctt ttagacatac cgcccgcgta tttatcgcct aagtcgcgtg
     5761 acgaactgtt ggcgcatttt gtataagcag ttagactttc tacgacgagt cacagagagt
     5821 ctatttttgc aaatttttca atgccttgca gcaaaagctt aaaacaacgg cagcagaccg
     5881 tggttgtctt tgcttgtcaa aatttcaaac gctgcacggt tctttgttgt aagctcgaag
     5941 gttaatccca accgtccttt ttcttgcacg gcgagggaac acgttgcgac tttggtgttg
     6001 aggcatgctt tgctgtcacc gggcggattg taatagcgca gtgcaacgac gtctttgcca
     6061 tcggcgtgga tatgtccttc gattttgagc gcatcgtttt cgccccaaaa tttccagtca
     6121 aaataatcca cggtagcttt attgcgtatc gattgccaat aaccattgag cgcatactct
     6181 ttgccttcat gcctcaccac aatgggcgtt aaatacggcg tggtgaaaaa tcccaattgc
     6241 attttggccg acgccacttc cagaaagctg tcgggtgcac cgtcaaaacc agcgacctga
     6301 ccccacgcgt actcgtcggt gtgacggctg ccccagttat ggttttggct gccctgccaa
     6361 tcggcgatgg cgattgtttt accgttgatg gtgagtgtgc ctttaaacat gacaaacggt
     6421 tgcgacacca agagcttagc gcgcggcacc ggtaaatcgt atgccgaaag cggcaaaggg
     6481 agaacgggat ttgtattttt cgcgtcgggc atccgttgta gcgacatgtt ccactcgatt
     6541 ctgttggggc caaggttgcc cgcgctccct ttcaaggcat agtcgttgag cgttgcgccg
     6601 tcgaaattca gattgaaatt tgtgttcgag taagaaatag cgctcagtgg gatttccgat
     6661 tttgcggcga tgggcatttt tcctttatca aaatataccg cccacaattc gcccatcggc
     6721 gctttggtgt ttgcattttt ctcactttgg aaaatggtat agcgtatcca gaaggcacgg
     6781 ccggtggcag cgtcgttggc gcgcatgaaa taactttcgt aatggccgcc cgaggcaact
     6841 cccttaaaac gggtgaaatt ggctgtagcg taggtttttt cgagttggat agacataggg
     6901 ctttattgca aaagtgaata cactgtcatc aaaattatga catcagtgtc attaaaataa
     6961 tgatgcaaag tgtatagaag cctgtaagcc aactcgttga gataattttt ttgcacgctg
     7021 tgcgagttgg cgcacaaact ctaagctaat ccattttttc ctttacaatc gattggtcgg
     7081 tggaacaata tattcttatg aaaaaaattg tgatagtcct cgttggattg tttctcgtct
     7141 cgtcgttgag cgcagtggat gacgcactcg caatcgcacg gcgtgaaatc ggcgtgcagt
     7201 tcgatttact caagaaagga aaaacgacac aacttaaaac gcattttacc gaccgccaaa
     7261 aagcgcgtgt tgtggatatg cgtgtcaaga gggcacaaaa agagacggct caatataaaa
     7321 tcaccgacct cgtccacagc gcccaagaga tctctcacaa tggccgccgc acaatcaaag
     7381 taaaaatgcg caacgaacgc actctcacca cacttgtgga aatcgacggg aagtggtacg
     7441 ccgatacggt gtggtttaaa taatcgaagc gacgacggca aggtcttgca tctggccccc
     7501 tctacgaagt gagaatatgg catccttgcc agccgcacaa cggggcacat cgtgtgccct
     7561 cgacaggaat cgaacctgtg cacatggctt cggagaccaa cgctctatcc actgagctac
     7621 gagggcaaat tgtgaagacg tggctaattg tgctgtcgag atgccgcgta aagaagtctg
     7681 tacataaatc gcctgagagc ctgtgcgccc gcttaaaata aataattatt tacatatagt
     7741 aagaagtttt agcccgtgat gtttatgaaa aaattagtaa tgatactcgc tgctgtaagc
     7801 ttcttagcag gctttagcgt tgcagcaaaa acaaacaccc aagtaaaaat ggaggcgcgt
     7861 gcaaaagcgc aaaaagaaaa atctgcacaa aaacgcgctg ccaacaaagc aaagtctaag
     7921 aagcgttgag ctgatttaga caaacttgtt tggagggggt ggctaacgag actgtctaat
     7981 tagccgctaa cgcacatatt gggaacccgt gtaccaattc ccgcagagtg tacagagagg
     8041 caatttttct gtgcaaattt ttgtacgcgg ttcgctcaaa aacacaaaat tttgcacaaa
     8101 ttcgggcgtc ttcgccccgt gcagagcaaa ttgaaacaat ttgccatcgg tcaaagagcc
     8161 gtcgccctca cggaattcta cgccgctttg gccaaggaat ttgtgcaaaa tagcgtacaa
     8221 aaatttgcaa aaaaaattgt ctcagcgagt tagcgcacag gctcttagat aatcccgatt
     8281 agtcatcgcc aaattataga atgataaaat ccttctttga tgctattaca aactatggcg
     8341 gcgaatctcg ccctctacag tttgcgacgg tttgtgcgtt tgcaaaacct tgtcattgcc
     8401 tgcgcagctt tagcgtttat tctttttcct ttcttactcg cgctcctcat cggttttgac
     8461 acaggccttt tcttttatgt attggcattt ttgccgattg gatttttagc ttttccaaac
     8521 aacattttcc gagctgcgac aacgcttgct atcaatatcg cctttttggt ggcgatgcat
     8581 gtgctcgcgt taaagtggaa acctcactat cccgcacccg attacataaa aattgaactc
     8641 aacgccatcg tcatcgtttt gagtattcta ttgttcggta ttcaaatcct ttacttggga
     8701 tttctcactc aagtgttcca gcgcattcgt gaagtatgga tgcaatatgc ccaactgggc
     8761 gctgaaaaat acaccgataa aaacagaaag cgcaaccaca ttatctccaa ccaaatcatt
     8821 atggtattca ccatcatact tgtcgtcatg gcggtggcgc aagcggttct ctcaattata
     8881 cttctcgtcc gccaatttga aacgtatata tattactcgt tgtactatct tgtgccgacg
     8941 cttctttgta tttttctttt tctacaaatt tttcacctta aagtaaaatc gaacactaac
     9001 acccgctttg aagcaattat ttttaccaca ggcaatctct acactcttta cctcgcttac
     9061 atccaaggcc gtgaaattgg tttgcactat gtttttctgc cgcttatttt agtgcctaca
     9121 tttttttcaa tcgcgtctgc gcgcttcaaa tacgcgatga cgattttacc catcactttt
     9181 ttggactctg ttttttcacg ctgcaagacg gctggcatcc cttaccccac gacattacta
     9241 aatctgtcgg tgaaatactc acgtttagta atatcgctat ttttttcgtt actctcgttt
     9301 atctttggat taaatcgcga cttaaagagc gatttttcgc tatttggcgc cagtggagtt
     9361 tgcgcggtgt gcatttcttt gattcggaaa ttgatagaaa ataccgcatt atgcaaaacc
     9421 aattactatt ggtgtttatc gtcctcacgt cattcatgat tatcaacgaa attaaaattt
     9481 tagtttcgtt gatccaactg ggctttaacg acacgttggg atttatcctc cattatgttg
     9541 tcccaacgtt tttttcttta ttactactca ccgcttgctt ttatgcaaat caatttaccc
     9601 gttcctttgt gcccatatca atagcatact tgtgggggat attgctgctt ggctatgtcg
     9661 gtatatccgt gcaagccgat tcgttttttt atattttcgc actggcgctt ttgcctttac
     9721 cttttattgc tctctcgggt aaaaccccgt gggaaaaaat gcttttgggt ttggggtttt
     9781 cggcattagc tttaatattc ttttctcgca ttgatttttg caacggtact caccgctttt
     9841 ccctctgccc aatgacgcga ttatcgcgca gttaggcttt gttgtcatgg caacgctgat
     9901 tgccactttc atcgtttatt ttttctacac cactgcgcag gcacgccttt ctgaatcgga
     9961 aattgaaaca gaacgaatga aatctgacca gcttttactc aacatcttac ccattgatat
    10021 cgcccgtgag ctcaaagaca aaggagccac cgaacccata cttttccatt cagcaactgt
    10081 cttttttacc gactttgtcg gctttactaa aatcaccgaa acaatgactc cgcacgagct
    10141 cgtcggtgaa ttggataaat gtttttcata tttcgataac gtctgcgaac actaccgatt
    10201 ggaaaaattg aaaacaatcg gcgactcgtt tatggcagca ggcggcatcc ccatccccaa
    10261 caacacacac gccatcgact gctgccttgc cgccatggaa atccgagact tcatggacca
    10321 gatgcagcaa attaaagaac agcaaaatca accgtactgg cagttgcgca tcggcatcaa
    10381 caccggcaac cttgtcgctg gcgtcgcggg tgaaaaaaaa attcgcatac gatgtgtggg
    10441 gagacacggt gaacactgcc agccgcatgg aatcggcagg tgttcctgga gaaatcaaca
    10501 tctcagaagc aacgtatcta caagtgcgta aattttttgt ctgcaaaacg cgtggtaaaa
    10561 tttcggcaaa gaacaaaggt aagattacga tgtatttttt acatggtata aaaacagagt
    10621 attcggttga cggtaaagga cgcgtgccca acgaacggtt caaagaaatt attgcggccg
    10681 agcgacttta actaactcca tcaccactgc atcgctgccg tcgttgtagt aactccgacg
    10741 gcgggctaaa tccacaaacc tcaacttttg ataaaacaag agcgctgcgg tatttttttc
    10801 actcacgtcg atgaggcacc gctctgcagc caacgcgtgg agcgcatggt tgattaaccc
    10861 actcgccact tttctacgcc gaaacgaagg aacaacccct actttcacaa cctcggcatc
    10921 cccaccgctc aaaagaattg aaataaaacc aacaggttcg ttttcttcgt atgctagata
    10981 aacatggctc gcagggttga ggagctcttg atgccaactt tcgacagaat acgctgcctc
    11041 tctaaaaata atttgatcga ggcggagtaa atccgcccga ttggtacgcc tagctttatg
    11101 aaaagtcagc aactaaaaaa ttttgtcgat gatgtaggtc gtgatttgta aagcgaatgc
    11161 actaatgaac aacgcttttt tagacttaat ggtgagcttg tcggtaaaac ggtcggcaat
    11221 ccaaaaaata atcacgttca agatgaaacc cgcaaggaga taaaaaagtc ccaacgtcaa
    11281 aaccatcaac aaccacttaa caataccaag tgtaataaaa ttgagcgcgg taaaaatcca
    11341 tgtcaccgca taattgacgc cgaccaaaat gagcgcaacg ataggaatag ctccattgga
    11401 aatttgaaaa tcagatatag cgctgcttag cgcatagagt aaaaagcaaa aagctgcaaa
    11461 tataataatg agtttaatta ataacataag ttggctacta tgctgcttca acaacgatga
    11521 gtcaacctaa aaatataaaa aaactgaata tctcttgaca gaattaaaag gttaatttaa
    11581 aataagagcg tgagcaaaaa agtaaatatt tttttagttg taggcgtttg ctttatagcc
    11641 tttttcagtt catgtgcctc cattcccgaa aaagcgaaac ctattgagaa ttttgatgtg
    11701 aatcgctact tgggtacgtg gtatgagctg gctcgttttg attttcgttt tgaaagaaac
    11761 ttagacaata cttcagccca ctatgcctta gataaaaaag gtaatgttat tgtattgaat
    11821 agtggctaca attatgtaga aaagaaatgg agtaaagccg ttggtttagc aaaattcaga
    11881 gatgcaaaaa atgtggctgc tctaaaggtt aacttctttg gtccttttta ttcgggttac
    11941 aatgttatcg ctttagacga taattatcaa tacgccttga ttgcaggtaa aaatttagac
    12001 tatttgtgga tactatcacg aacaaaaaaa ttacctgatg aaattaaagc gaagtatttg
    12061 aagatagccg aagaaatagg gtatgatatt tcacgattga tttgggtaaa gcacgacaat
    12121 gacaataatc catttctgaa tgagaactaa aataaccgta ttttggtttc gacgtgattt
    12181 gcgcttaaaa gacaataaag gattttttga agcactttca gaaagctttc ctgtttatcc
    12241 gatatttatt tttgataaaa atattctgac taaattagaa gataaacacg atcgaagagt
    12301 cgattacatt catcaagcgc ttacaaacat taaccatgaa ttaaaaacag ttcaatcgac
    12361 attgaatact ttttatggta atccgttagc tgtctttcaa caactttcaa aagactatgt
    12421 tattaaagcg gtctattgta atcgagatta tgaacccagc gccattctcc gagacactga
    12481 gatttacaat ttttttaaga atcaaaacgt tccgtttaag gcattcaaag atcaggtcat
    12541 ttttgacaaa aatgacgttc taaaaaacga cggagcacct tatactgttt atactccgta
    12601 ttctaaaaaa tggaaagaac gtttgacggt actccattac aaagcgaata catacacatt
    12661 aaaaaattta tataaaaata aatttacaga aattcattcg ctcaaagacg ttggttttga
    12721 aaaaacagaa attactttta aaaaaccaga attaaatgtt tctataatta gcgaatacga
    12781 tgcataccgc gattatccag caatgggacg aaccactcag ttgggaatta cattgcgttt
    12841 tggaacgata agcgttcgtg actgtgtggc atttgctgta gagcataatc aaacgtggtt
    12901 gaatgaactc atttggcgtg aattttttat gcagattctg tatcattttc caagagtagt
    12961 atatcaatct tttaaagaga agtatgatta tatccaatgg ctcaataatg aacgcgaatt
    13021 tgaattatgg cgcaaaggaa aaacaggcta ccccatcgta gatgcaggaa tgcgcgaact
    13081 caatcaaacc ggttatatgc ataatcgagt gcgaatgatt gccgccagct tcttatgcaa
    13141 acatttgctc atagattggc gttggggcga agcttacttt gctacaaaat taaatgatta
    13201 cgacttatcc gccaacaatg gtaactggca atgggcggct ggttcgggtt gcgatgcagc
    13261 gccgtatttc agggtgttta acccgacatt gcaaaccgaa aaatttgata aaaatttaga
    13321 atacatcaaa aaatgggtgc cggaatatgg aaccgaagaa tatccgaagc ctatgataga
    13381 gcacagcttt gcccgagaaa gagcattgca aatgtataac caaggattag acagaagtaa
    13441 aaattatgag taaagtttta attgcaggag caaccggttt tatcggcagg tatcttatcg
    13501 gttatttgtt agaaaaaggg gattctatcc atgcgttaac aagaagaaaa caaaatagcg
    13561 agcagtcgaa tatctcgtat ttcgaatggg atgttgcgac tggatttatc gatggtaatg
    13621 cttttgataa ggtaagtagc atcataaacc tcacgggcgc taatattggc gatcaacgat
    13681 ggacaaacaa acgaaaaacc gaaattttgg aaagtagaac gaaaccgatt caattgttat
    13741 acaactatgt tcgcgaacac agtttgaaga ttgacgcgtt tatttcttct tctgcggttg
    13801 gctattatgg agccgttacc tctgatgaag tttttatcga aaattcagtc aaaggcgctg
    13861 attttttatc cgaagtgtgt caaaaatggg aacacgccgc ttttcaattt tcagctttag
    13921 gggcaagaac ggtcgttctt agaaaaggcg ttgtcatcgg taaagatgga ggtatttatc
    13981 aaaagttagc gcctttggca aagcgaagct taaatactgc tttgggggat ggtaaacaat
    14041 atttaccatg gatagatgta cgagatgtgg ttcggttgta tgaatttatt ttaacgcacc
    14101 gcgaagtttc gggtgtttat aatgcagttg ctaccgaaca tataacgatg aacgattttt
    14161 cagaagcctt attggcctca tttggtaaaa aaagtattct gcctaatgtt cctaaattta
    14221 tgattcagat tgcagtcgga gaaatgtccg ttatgctgtt aaaaggctcg aaagtgagta
    14281 accaaaaatt gagaaatact ggttttgaat ttgaatatga aaagattaaa aatgctcttg
    14341 atttgtaatc ggtttagatt ttaaggtgtt ttccccggaa ttatgtcaca ttagggggta
    14401 actatcccta ccgctgcagg gataactatt cctcaaaagt atgggagaga ctcataagta
    14461 atcgatcctt ttttggctta ttgctctgcc gaaaaacgat aatattagag gacaaatgtt
    14521 tttaaaacag atcgccttaa tttccgcttc gactctcttt atactttcct gcgcgcggtt
    14581 gggcgaagca aaaagtctca cgagcgcgca agaggcggtc gcaacgcagt taatcatacc
    14641 gaccgatgca acaaagcccg gcatggggca agatggcggc cctttgactt ggtcattttg
    14701 ggtaaaaaac cgccaagcgt ccgccgaact catgctagga agcttcaccc cattctataa
    14761 aattaacacg ttacgccgcg cggtttcaca acactttgtg ctttatgccg acacggcgag
    14821 cgccgcgctg gtttcttcga gcgtaaaccg tatcgccgtg ttgacctctg gcagcacgac
    14881 tgtcgcaggc ctttccgcca caggcgattt agccgtcggt atgcgcgtta ccggcggggg
    14941 attaccctgc gccacgtata tcgcgagcat caccaacagc gctgtagtag agattactaa
    15001 tcccgcgcca acaagcggta caccgacgct cacgttcgcc catggcccca gcgtcatcct
    15061 cgatagtatg gaatgcgcct actcaaattt actccatgtt tatggagggg ataaacaccc
    15121 gtacgcaaac aataatgcgc ggatagttat ccttgcgaac gacattttag acgattacgc
    15181 aaccactggc ggttatgtgg ggggatattt tgccccgcgc gatttatact cgaatgcgtt
    15241 caccacatcg ctttttacca accctgcggc gatccagcaa aacgcacagc tcgtgggcca
    15301 gttgggtggc tatagcaatg aaatgagcgt cgtctattac gacttaaatc cgggctacac
    15361 caccgcaccc aaccaagtga acgatatcgt gttgcatgaa ctctcgcact tgttcacgta
    15421 taactaccgc gtcatccagc aacgctataa aaatctcgat ctgtggattg ccgaaggggt
    15481 ggcggaaaat gctccacccc aaacgaatgg ctctggcaat ttgctcacgc tgcgccttag
    15541 tcaaatgggt tcgccagcgc tttttgcaag ttaccaagcc gcgccgcaat tggtcgattt
    15601 ccaaacgtgg ggaccaaaaa ttgtgggcta tttacaatcg aatctctttt ttagttatct
    15661 ccgccatcga ctagagctca atagcacggg tagcgctgcg tcgttcgtgc acgatgccgt
    15721 cacgcaaatc gacacgtcca tgaatgggat taacgcttta attagtactt atttaccgag
    15781 tgaaaatttc tccaccattt atcaggattt tgtcactacg cattatttaa tgctcttggg
    15841 gatacagatc gaggatacca acggcatgaa cggcggagga atcaacctac aacaaaaata
    15901 tagtttttgt aaaagtgaaa tcggtatctc gaatacggga gtgaataatt gcacgagtat
    15961 caaagcgcgt tactcctcaa acatcccttt taattacgat gcgcctcgat gctccgacaa
    16021 tacctatggg ttaaaaccca attcgtatat tatttaccgt cacaaggtcg ccggcgaccg
    16081 cactcatttt gatatccctg gcacagatcc cgtggcaggc gaactcccgt taaaaattat
    16141 catcaacacc acccccgaag caacgcttat cagcggacca ccggcaacgg ttaatttgta
    16201 tacctacaac gccggcgata acattccttt tacctcgagt ttgggttttg tgaacggcga
    16261 cattgcgcaa atcatcgtgg tgaatcccaa cccaacgggt agttgtcgta aactcgacga
    16321 aacgtggtta ctcaaacgca accatagtaa gtgggtgggg caaaactctt ttggtgtgca
    16381 cgcttctccc gatttttact ggcaaagcga tgcgggggct tcgtggctga ctgacaccga
    16441 tggcgcttat tatcgccctg cgggccttgc cgccgattta caaggatatc ccaataatcg
    16501 gctttatatc agcgactaca tcaacatgag cgtgcaacgc ctcaatttgg atacgggtga
    16561 gcccctcggt cgtttgggga gtccatcaaa tacatgccca acaaccggta tgccgtggag
    16621 tacatcgaca aaccgttact taaacgggta ttgtgcgcat aacttcaact caccacaagg
    16681 attggctgtg cacaaggatc ataacctcta cgttgccgat accgata
//
LOCUS       DAOMPD010000006        14672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1768_length_14672_cov_3.333174, whole genome shotgun sequence.
ACCESSION   DAOMPD010000006 DAOMPD010000000
VERSION     DAOMPD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1768_length_14672_cov_3.333174"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLY93_00505"
     CDS             <1..754
                     /locus_tag="PLY93_00505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRP67999.1"
                     /translation="EKFFAARETRLQSTLVALLATDDHPLSGKAAAEAQKLAGTIEAF
                     AKKTTTDLGFFLDELLEKTGLDKLYEEEDRLLQSGRMENIAELRNSLLLYQRQSENAS
                     LGDYLQQISLLTSTVDDDEAKETVSLMTVHNAKGLEFETVIIAGFEKNFFPHYLAERD
                     DTVSEERRLFYVAVTRAKNQLYFTSAQRRMAQGSYESTQPSPFLREIPLDLIRTEKKY
                     DTSNFSSPVKKSFMPSAFGSGNKKSIDFHSHF"
     gene            840..977
                     /locus_tag="PLY93_00510"
     CDS             840..977
                     /locus_tag="PLY93_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68000.1"
                     /translation="MEHVNFGKGRVLRVEGDGDGARIHIYFDDGKTRKFLLKFTQLKR
                     V"
     gene            complement(984..2168)
                     /locus_tag="PLY93_00515"
     CDS             complement(984..2168)
                     /locus_tag="PLY93_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRP68001.1"
                     /translation="MAYKYDVDALKADLEMLLGNPWYRATELEVRQLIEANLRQGGSV
                     AQAAIFNAWKSEKQYHANTAPPLAQQESRSYYQEQKPKLQKIFLETRSNPNNHYRIPL
                     PLGDIPRRKPEKVVYKMDAYFTTLEQAMRLRNYARATMRQYEFLLRHFAAYCDALDEK
                     FSPAVNEKLVVDYMLKRRDDGWSTTTLCTFRAALRLYCEVNGSPRDFAFIRNMKKEKR
                     LPVVLTASEVARLLATIKNPKHWLMVSLMYASGLRVSEVVRLRVQNVDCEKHTLTVRQ
                     AKGRKDRVTVISEKQVALLGEFMAGKNKGDLLFTSSHAPHRALAVRSLQKVVERALLH
                     AGIRKGASAHSLRHCFATHLLENGTDIRFIQKMLGHSSLSTTAIYTKVATHQIGKIKS
                     PL"
     gene            2949..4157
                     /locus_tag="PLY93_00520"
     CDS             2949..4157
                     /locus_tag="PLY93_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68002.1"
                     /translation="MTNKTDKYSAPQQALGYMYQPRYALHRILMLPEDTTCLLEADDD
                     IDFNDPEEGKLLASLKHKAPGDKLTDLSPDFWKSVRIWATFVIANPNRISDTSFFLIT
                     TGEIQIGSFLSNFIAGNDERKAPDKLRETAMEVISKSDAKLLKSIHAQLISMDVRLLE
                     KLFSKITVFDCQERIDDIPSKIMALLRAVRPQHRKPVFERLEGWWNDQCVNIMTGKRS
                     EPLLGSEVSEMLAHISEQFRDDNLPLDFFDAEPAEGVDPERDNRIFVHQLREIGLKSD
                     RIRRAILDYYRAYEQRSVWARENITITGEVERYDDRLVDEWQRLREVVCEELEAESPE
                     EILCQAGRKIYTDLSNGLNPNLRIRPQVTESYVTVGSYHILANEPTPRVYWHPQFLNR
                     MQNILEAVDK"
     gene            4154..4642
                     /locus_tag="PLY93_00525"
     CDS             4154..4642
                     /locus_tag="PLY93_00525"
                     /inference="COORDINATES: protein motif:HMM:NF039805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6521 family protein"
                     /protein_id="HRP68003.1"
                     /translation="MKAWNNRPRELRNLFNPAFLGVIMLRAIHEYGNENGKPMPFSLC
                     LLIPALSLHKDTREILRKGIKTYFVKIVEDNPKLLVELPDRLRGLFPYTMDSLGYLLS
                     QGLIVINENGHISAIEGSVQKTISGTQETQDCQKVARSLGRKFAQISDRATVYTMLGI
                     RP"
     gene            4639..6579
                     /locus_tag="PLY93_00530"
     CDS             4639..6579
                     /locus_tag="PLY93_00530"
                     /inference="COORDINATES: protein motif:HMM:NF023946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3732 domain-containing protein"
                     /protein_id="HRP68004.1"
                     /translation="MKIKKILLYSHKGELRQLTFNLQGLNIITGRSSTGKSAISEIVE
                     YCMGRSTFQVPEGTIRDKVSWYSVIFQFTGEQVLIAKPAPASNASSCSRAMIRRGGNI
                     EVPEFGDLLCNADDDAVVSLLSEMLGIPENQTAVATENSRSSFSANVKHTFYYLFQKQ
                     GLIANKDLLFYRQNEPHIPQAIKDTFPILMGIAPDDSYEKETKLRTARRDLKLLQRQL
                     SDAQDFSDQLNIRAVALLSEAQQVGIVSMGAMPDTTEALLSALRDVESWKPAPVPDED
                     VARIVSIEDEIDQLRKLRRSLEENLKATIHFTERENGFTDEASEQKSRLESIKALPRN
                     RQTGEWQWPFSEPNLGMQTSIAQAILGELKSLEIELEAITGERPKMEKLIRELTEERD
                     RVSERYRAKHEELAAAIAANEKIAQMGNRNAAAARTVGRVSLFLETYTPENDFRELEE
                     RIEDKIRQVKLLENNIGAENSQERLISILNIISNQMRTYSEYLDAEFSDRPLRLDLSR
                     LTVVIDRNERPILMNNTGGGANHLAYHLAALLSIHHYSFTHSRPIPSFLMLDQPTQVY
                     FPSEERYKASSGSVEDTERDSDLEKVRSLFEMLYRFCTAECKGFQIIVSEHANMRDKW
                     FQESLVEEPWSKPPALIPDDWL"
     gene            7139..7519
                     /locus_tag="PLY93_00535"
     CDS             7139..7519
                     /locus_tag="PLY93_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68005.1"
                     /translation="MRLVRYIFSFYFCTALPLSAFSQVHYDHSINEGHAPHICFGENT
                     DCDCATEKHAEESLWEHVFESGCLKTLSVQLLEPVLSVFAPPLSLDFKPQVIIYYLPE
                     SPDLLFEPPIIGSPPSRAPPSISI"
     gene            7639..8790
                     /locus_tag="PLY93_00540"
     CDS             7639..8790
                     /locus_tag="PLY93_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP68006.1"
                     /translation="MAVSQANSAFIDAKNLQIKAAGLMADQARLWGNPTLSGDFGGIT
                     QNDISGFSYSFRISQPVFFPGKQRLRASLAAHNQQFEMLNLRERKQLIQFNVIQSAYR
                     YKLTEDMLKEFQARLNRFKMLRLAIASKPAASPEIRAQKYIIENALRNSERDYFNLQK
                     ELAIALQNLQVYFDKTENLSLELPLFSGNAALDREPLLTEVLANNLLIQKIGVQVAQA
                     ETNSTLLSREAYPDISIAAYYDKDTGPFQEYRFGGGLSLPIPLFNRNQHAAAAADEYA
                     KAARKEREYALSILKRDFNAVFEEYLVSLRLIEKFPIDQVASTNRNLKSLTAEFIRSR
                     LATLTYADAEQRMQDSIVSAYMAQMAYVNAVLQIALFRGSDDFAAYFHK"
     gene            8800..11937
                     /locus_tag="PLY93_00545"
     CDS             8800..11937
                     /locus_tag="PLY93_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRP68007.1"
                     /translation="MLSKIVEFSLNHRLSIIVTLVAVLGGGIFVGFQLPVDAVPDITN
                     VQVVVNAKTGGLDPQQVEKSVTYFIETEIAGIPRVQEIRSLARFGLSQTVVIFKDGTD
                     IYWARQQVGEKMRNASDRLPAGVSMEMAPISTGLGEVLMYVLLPKSGSELEKKSELER
                     LLYLRTIQDFVLRPYIKSNAIGIAEVDSLGGFKKQIHIDFEPARLEQYGVTFDEIVQK
                     LKTVGENFGGGYIEKDTQQWIVRTKGQVSLKQFEQIPVRNNIFGSPVRLKEVATIRED
                     HAQRVGAATYKGNEAVLGTVLMLMGENSREVALNAEQVIKNAPLPDDVVAEIVYTRSF
                     LVNATLKTVAKNLAEGAAIVILILLLFLGNLRAALFVSLSIPLAMAFALIGMKVFHIS
                     ANLMSLGALDFGLIVDGAIVMIENLLAKMEHQPPKNIAQKLKMAKDAALEVLAPMTTG
                     MIIIMSVYIPILTLEGTEGKLYYPMAMTVIFALTGALLMAIVAIPVLGYYVRVKSEPQ
                     ATKLFQAVYKAYEPILNFPLRNIRKLVLPVVAFLLICGWIFARLGSDFMPPLNEGDMA
                     LNLVHDAKISLSESLTRQRKIESKLATYPEIERVFSRIGTSEAATDPMGVNLSDLFVI
                     LKKDTSQWRKDAKGKTLDKDGVFKLIEADAKELLEKTDKLAESELVQTQPIAMRFNEI
                     LEGSRADISLRIYGSDLDTLAELQDRSVEILKKIKGAREVELDALTALRKSLVFDASI
                     KYNKVAYYGVSLGDVNFSLQTAMAGMTIGSYYEQAWQFPIVVRLSEENREKLSEINRI
                     PIALPTGGTIPLSKVAEFSVKENVTSIARSGLERYAGVAVFLGDRDTLSFVEEAKAKI
                     KEELKIPKGYRLSWGGQFKNLERARVRLAIIVPLILLVILFLVYRTFHSIKQTLLIFL
                     TIPFAWTGGVLALWFTGIHFSVSAAVGFIALSGVAVLNGLVKVSYLNQLRAQGKSVNE
                     AVHIGALGRLRPVLMTASVASLGFLPMVLNTGIGAEVQRPLAIVVLGGLITATLMTLL
                     LLPSFYQWMEKGDEK"
     gene            11934..>13713
                     /locus_tag="PLY93_00550"
     CDS             11934..>13713
                     /locus_tag="PLY93_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRP68008.1"
                     /translation="MKQVFKDKKFISLIVAIAIVVSLEVLSLLGIHIPMPYAPFVFSA
                     FIIGIGYGVIWKGLKALATLRFSSINLLMLIAVIGAFYLREFPEAAVVIVLYVLGERL
                     EDIGIEQSKSALDELVSKSPKTAVVKAIGEAVEIDTIPIGTIIAIKPGDLIPLDGKIT
                     GGETAVDEAAITGEPIPKDKRPGDLVFAGTLNKSGYIEVETTKLATDTTFAKIIKLTF
                     EAQANKSDTQKFIQKFASIYTPGIILLATGLLIVPVFLLKQDFSHWLQQAITLLVIAC
                     PCALVISTPVAIFAAIGNASTKGALVKGGKFIEALARIRVLALDKTRTITFGNPIVSD
                     VIPLNGISREELLACTAGAEIFSEHPLAQAVVDAARREGFEPHRAELFKSIAGKGATA
                     KCLVCEDETIYLGKLSYIHESQPSDAEAENIVKRLSGQGKTSVAVSFGQGVAGIIGLA
                     DEIKPDSAAAIDELKAIGVEPVMLTGDSEHAGKYVADKVGIGSVYGSLMPDDKAEQIK
                     RLRAQYEYVGMVGDGINDAPALAISSVGIAMGAAGSDAAIESANIALMNDKLSLIPFL
                     IRLSRAALLRIKINTVAAVFVKLAFVG"
     assembly_gap    13714..13723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13836..14537
                     /locus_tag="PLY93_00555"
     CDS             13836..14537
                     /locus_tag="PLY93_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRP68009.1"
                     /translation="MNEILIRETPILDFNHPSLKALIDRCGWKALSERDRILAIYNFV
                     RDEIQFGYNVSDDIPASAVLADGYGQCNTKGNLFMALLRAVGIPCRFHGFTINKELQK
                     GAITGIWYRLAPQEIVHSWVEILFEGKWRNIEGFILDKQYLTALQVRNPEVRGYFCGY
                     GVATDNFANPQVDWNGGDTYIQKEGIKQDFGIFDSPDDFYRGHRQKLNWLKRLIFRWL
                     TRHSMNRNVSRIRRN"
BASE COUNT         3921 a         3463 c         3552 g         3726 t
ORIGIN      
        1 tgaaaaattt tttgccgcac gtgagacgcg gttacaaagt actttggttg cgctccttgc
       61 caccgacgac catccgctct cgggtaaagc cgccgccgaa gcacaaaaat tggcggggac
      121 tatcgaagcg ttcgccaaaa aaacgacgac ggatttagga ttttttttgg acgagctttt
      181 agaaaaaact ggactcgata aactttacga agaagaagac agattgttgc aatcggggcg
      241 catggagaac attgccgagt tgcgcaattc gcttttgctt taccaacgcc aaagcgagaa
      301 tgcaagcctc ggtgattatt tgcaacagat ttctctcttg acctcaacag tggacgacga
      361 cgaggccaaa gaaacggtga gcctcatgac ggtgcataac gcaaaaggac tcgaatttga
      421 aacggtcatc attgcgggtt ttgaaaaaaa tttttttccg cactatttgg ccgagcgcga
      481 cgacacagtg agtgaagagc gtcgactttt ttacgtcgcc gttacgcgtg caaagaacca
      541 actttatttc acgagtgcgc aacgccgcat ggcacagggc tcttacgaat cgacgcaacc
      601 ttcgcccttt ttgcgtgaaa tcccactcga tttgatacgt acggaaaaaa aatacgacac
      661 gagtaatttt tcttctcccg taaaaaagtc gttcatgcct tcggcattcg gtagcggcaa
      721 taagaaatcc atcgacttcc attcccactt ttgactctcc tcaaccttcc aaaacgccct
      781 cattcggcag cgcagttact ccccaaaaaa gcggcgctac gttcgcctcg ggtgaaaaag
      841 tggagcacgt caattttggc aaagggcgtg tgttgcgggt cgaaggcgac ggcgatggtg
      901 cccgtatcca catttatttc gacgacggca agacgcgtaa attcttactc aaatttacgc
      961 aactgaagcg tgtataaaat aactcaaagt ggacttttaa ttttaccaat ttggtgcgtc
     1021 gcgactttgg tgtaaatcgc cgttgtcgaa agcgaactat ggccgagcat tttttgaata
     1081 aaacgaatgt cggttccatt ttcgagcagg tgcgtcgcga agcaatgcct aagcgaatgc
     1141 gcactcgcgc ccttacggat tcccgcatga agaagcgcgc gctcgacaac cttttgcagc
     1201 gagcgcacgg cgagcgcgcg gtgtggcgca tgcgacgaag taaagagtaa atccccctta
     1261 ttttttcccg ccataaattc accgagcaac gccacttgtt tttctgaaat caccgtcacg
     1321 cggtctttac gtcccttcgc ttggcgcacc gtcaaagtat gtttttcaca atccacattt
     1381 tgtacgcgca aacgcaccac ctccgacacg cgcaaacccg acgcgtacat gagcgaaacc
     1441 atcagccaat gcttgggatt tttgatcgtc gccaaaagcc gcgccacctc ggatgcggtg
     1501 agcaccaccg gcagacgctt ttcttttttc atattacgaa tgaatgcaaa atcgcgcgga
     1561 gaaccattca cttcacaata aagcctgagt gccgcacgaa acgtacacag cgtcgtcgta
     1621 gaccatccgt catcgcgccg cttaagcatg tagtcaacaa ccaacttttc gttcaccgcc
     1681 ggtgaaaatt tttcatccaa cgcatcgcaa tacgcagcaa agtggcgcag caaaaattca
     1741 tactgacgca tcgtcgcgcg cgcgtagttg cgtaaccgca tcgcttgttc taaagtcgta
     1801 aaatacgcat ccattttata aacaactttc tcaggcttgc gccgtgggat atcccccagc
     1861 ggcaacggga ttctatagtg attattcgga ttgcttcgtg tctccaaaaa tattttttga
     1921 agtttgggtt tttgctcctg atagtaggaa cgactctctt gctgcgcaag cggcggtgcc
     1981 gtgttcgcgt ggtattgttt ttccgatttc caagcgttaa atatggccgc ttgcgccacg
     2041 ctaccgcctt gacgtaagtt tgcttcgatg agctgcctta cctcaagttc cgtcgcacgg
     2101 taccacgggt tacccaagag catctccaga tccgccttca acgcatcaac atcatactta
     2161 tacgccatac ccaacaatat acacgtaaaa cattttgtaa actatcatta tacgtataat
     2221 atcttacact ttaatctaaa aatgtcttga ctgtcacaat taaatgcaat acagagaaaa
     2281 atagttaggt tcaattgccc cgcccttcct tgggaaggca aatatggaaa gccgacctcc
     2341 atgtcggcta aacgaagtta atgaaaggag taaacaaaat aatgggtagt tccgtttata
     2401 ataaatttcc gtcttttaag tgggaaaaat ccacattatg gttcgtatct aagactatgt
     2461 tctacaagaa attatgaaaa tcattgcatt acggggaaga gcgaattcag gtaagacaac
     2521 cacaatacat atgttgcatt caatcctact taacaatgga tactgtcaag taccaggagc
     2581 gcgcaagaat tttggaagag atttcttaga tgtatacctg agccccaaga atgaaaaaat
     2641 aggtgttact agttcaggag atactcatga ttctgttaat gatcgattgc aagatttaat
     2701 atcacaaaag tgcgcaactt gtgtatgcgc ttgcagatca tatgatagat tgccaccagg
     2761 aacagttgca gccactcaaa atttcccaaa ttatactccg cagtacattg caaagactat
     2821 aagtaaagtt gcatcacaag aacagcgaga caatataaat gatgcaaatc ggatatatca
     2881 atccttataa ggtatagcaa gtctgtgtac aagagtatat taacaatgat taaatgactg
     2941 tatatcccat gacaaacaaa acagacaaat attcagctcc gcagcaagca cttggctata
     3001 tgtatcagcc acggtacgca ctacaccgta ttttgatgtt accagaagat acaacgtgtt
     3061 tgctcgaagc agatgatgat atagacttta acgatccaga agagggaaag ctgttggcgt
     3121 ccctaaaaca taaagctcct ggtgataaac tcactgatct ctcacctgat ttttggaaat
     3181 ctgttcgcat ttgggcgact ttcgttatag ccaacccgaa taggatttcc gacacatcct
     3241 tttttttgat tacgacaggt gaaatccaga taggatcttt cctttccaat ttcattgcag
     3301 gtaatgatga gcggaaggca cccgataagc ttagagaaac ggccatggag gtcattagta
     3361 agtcggatgc gaaactcctg aaatcgattc atgctcaact tatcagtatg gatgtgaggc
     3421 tattggaaaa gcttttctca aagattacag tctttgattg tcaagagaga atcgacgata
     3481 ttccttcaaa aataatggca ctattaaggg ccgtgcgacc tcaacacaga aaaccagttt
     3541 tcgaacgcct tgaaggatgg tggaacgatc aatgtgtcaa tataatgaca gggaagcgat
     3601 ccgagccact cctgggtagc gaagtgagtg aaatgttagc ccatatttcc gagcaattta
     3661 gggatgataa ccttccgtta gatttcttcg atgctgaacc ggcggaaggg gtcgatcctg
     3721 agcgcgacaa cagaatcttt gtacatcaat tgagagaaat tggcctgaag tctgaccgaa
     3781 ttcgccgtgc aatcctagat tattaccgtg cctacgaaca gcgatcagta tgggctaggg
     3841 aaaatatcac gattaccggt gaggttgaaa ggtacgatga tcgcttagta gatgagtggc
     3901 aaagactcag agaagttgtg tgcgaagaac tcgaggccga gagtccagag gaaatactgt
     3961 gccaagctgg ccgaaaaatc tatacagacc tctctaacgg actgaatccg aacctcagaa
     4021 ttaggccgca ggtcacagag tcatacgtga cagttggtag ctaccatatt ttggccaacg
     4081 agcctacccc tcgtgtctac tggcatcccc aatttctcaa tcgaatgcag aatatattag
     4141 aggcggttga taaatgaagg catggaacaa tcggccgcgc gaattgcgca atctattcaa
     4201 tccggcattt cttggagtga tcatgctaag ggcgattcat gaatatggga atgaaaacgg
     4261 caagccgatg cctttctcgc tctgtttgct gatacccgct ttgtctctgc acaaggacac
     4321 gcgtgaaatt ttgagaaaag gtataaagac gtatttcgta aagattgtcg aagataatcc
     4381 aaagcttcta gttgaactac cggatcggct cagaggactc tttccctaca cgatggattc
     4441 actcggttat ctcctctcgc aaggcttaat agtgattaac gagaatggtc acatttcagc
     4501 gatcgagggt tccgtccaaa aaaccatttc aggaactcaa gaaacccaag actgtcaaaa
     4561 agttgcgcga tccttggggc gtaaatttgc gcagatctca gacagagcaa cggtctatac
     4621 aatgttagga attcgaccat gaaaattaaa aagattctgc tttacagtca caaaggtgaa
     4681 ttgcggcagc ttacgttcaa tcttcaaggg ctcaatataa tcacaggacg ttcgtcgacc
     4741 ggaaaatctg ctatctcgga aatagtcgaa tattgtatgg gtagaagcac gtttcaggtg
     4801 cctgaaggta caattcgaga caaggtcagc tggtacagtg tcatatttca attcacaggt
     4861 gagcaagtgc tcattgcaaa accggcccca gcatcgaacg cttcaagttg ttctcgagcg
     4921 atgatccgcc gaggcgggaa catcgaagtg ccagagtttg gtgaccttct gtgcaacgca
     4981 gatgatgatg ctgtggtatc gctactatcc gagatgcttg gtattcccga gaatcagact
     5041 gccgtcgcta cagagaacag ccgatcaagt ttcagcgcaa acgtgaagca cactttctat
     5101 tatctatttc agaagcaagg gttaatcgcc aataaggatc ttcttttcta tcggcagaat
     5161 gaacctcaca taccgcaggc gataaaggac acattcccaa tacttatggg gattgcacct
     5221 gacgattcat acgaaaagga aacgaaactt cgaacagctc gcagggacct aaaattgctc
     5281 cagagacaac tttcagatgc ccaggacttt tctgatcaat tgaatattcg tgcagttgca
     5341 ttactctctg aagcacagca agtcggcata gtatcgatgg gagcaatgcc cgacacaact
     5401 gaagcattac tttcggcgtt gcgggatgtc gaaagttgga agcctgcgcc ggttcctgac
     5461 gaagacgttg cccgcattgt ctcaattgaa gatgagattg accagttgcg caagcttcgt
     5521 cgttctttag aagagaatct aaaagccacc attcatttta cagaaagaga aaatggattt
     5581 acagatgaag cgagtgaaca gaagagccgt ttagagtcaa ttaaggcact ccctcgaaat
     5641 cgacaaactg gggaatggca gtggcctttc agcgaaccga atctaggaat gcagacatca
     5701 attgctcaag ctattctcgg cgaactaaaa tcattggaaa tcgaactcga agcgataacg
     5761 ggtgagcgac ccaagatgga gaaactgatc cgggagctga ccgaagaaag agatcgagta
     5821 agtgagagat atcgagcaaa acatgaagag ctagcggcag caatcgccgc aaacgaaaaa
     5881 atcgcgcaga tgggtaatcg aaatgctgcg gctgcgagaa cagtcggtag agtcagccta
     5941 ttccttgaaa cctacacccc agaaaacgat ttcagggagc tagaggagcg cattgaggat
     6001 aagatcagac aagttaaact gcttgaaaat aacataggag cagaaaacag ccaggaaagg
     6061 ctgatatcta ttctaaatat catctcaaat cagatgagaa cctattcaga atacttggat
     6121 gctgaattct ctgaccgccc tttgcgactc gacctaagtc gcctaactgt ggtgattgac
     6181 cgcaatgagc gtcctattct catgaataat accggggggg gggcaaacca cctcgcatac
     6241 cacctcgcgg cattactttc cattcatcat tattctttta cgcacagccg cccgattcca
     6301 tcatttttga tgctagatca accgactcag gtctatttcc catcagaaga gcgttacaaa
     6361 gcatcttccg gttcggtgga agatactgag agagattctg atctagaaaa ggtccgcagc
     6421 cttttcgaaa tgctctaccg gttctgcacg gcagaatgca agggtttcca aattattgta
     6481 tccgaacacg caaatatgcg ggataaatgg tttcaggagt ctcttgttga ggaaccttgg
     6541 tctaaacctc ctgctcttat tcctgatgat tggctatgat tcagaaccag gttgccagca
     6601 tgccaaatac attccggtaa acagtactgc tgattttctt tgctaactga ataagatttc
     6661 cataaaatga agagtgtgaa tttcaagctc gacatccgtg tctcgcttga agtgagggcg
     6721 gggcaactgc acctaactcg gtttagaagc tgcgggcggc gggtccccaa ccccgccgtc
     6781 ctcggtcgca acccttagag acatcccaag cgaagtggac tcgccaaggt gaaattttcg
     6841 gttgccggcc tgaaaggccg ggtttacttt gtaaacgggt ttgcgacaca tttctcatgc
     6901 gccggtgggc gcattcgaaa cgtcgtctaa accgggacgt taggcgaaca tgcctcgctg
     6961 atttttgttg ttacttccgg tcatccatga ccggttcttt cctgatagct tattttgttg
     7021 tcgccgtctc agtcatcgaa ggattttttt attgagaatc taatttcaac aattagatag
     7081 cgtgccgaat tcctgcttat tgctttaccg gctgtgtgat tttgaagaaa agagattcat
     7141 gaggcttgtc cgatacatct ttagcttcta tttttgcacg gcgctgccgc tttctgcctt
     7201 ctcgcaagtt cactacgatc acagcattaa cgaagggcat gcgccgcata tctgcttcgg
     7261 agaaaatacc gattgcgact gcgcaacaga aaaacacgct gaagaatcac tctgggagca
     7321 tgttttcgag agcggttgct taaagacgct ttctgtgcaa ctgctcgagc ccgtattgtc
     7381 ggtctttgcg cctccattgt ccttggattt taagccacaa gtcattatct actatttacc
     7441 cgagtcgcca gatctacttt ttgagccgcc cataatcggt tctccgccca gcagggctcc
     7501 tccttcaatt tccatctaac tcaccctttc taaatctctc attacccaga aggaggacaa
     7561 atgagaacta ttatattatg tttattcacg tgctcatatt tttcggtcta tgccgagcag
     7621 tatgatttga ggagccttgt ggccgtttct caggcaaatt cggccttcat agacgccaag
     7681 aatttgcaaa tcaaggcggc cggcctcatg gccgatcagg cccgcctttg gggaaacccc
     7741 acgctttcag gggactttgg cggcatcacg caaaacgata tttcaggttt ttcgtattcg
     7801 ttcagaatct cgcaaccggt ttttttccct ggaaagcaaa gattgcgtgc ctcgctcgcg
     7861 gcgcacaacc agcaatttga gatgctaaac cttcgcgagc gcaagcaact gattcagttt
     7921 aatgtgatac aatcagccta ccgatataag ctgactgaag atatgctcaa ggaatttcaa
     7981 gcccgcctca atcggtttaa gatgctgcga ttggctatcg catcaaagcc ggccgcctct
     8041 ccggagattc gggcgcaaaa gtatatcatt gagaatgcct tgagaaactc ggaacgcgat
     8101 tattttaatc tgcaaaaaga actggcaatc gctcttcaaa atctccaggt atacttcgat
     8161 aaaacagaaa acctgagttt ggagcttcct ttgttctccg gaaatgcggc gcttgatcgc
     8221 gagcctcttt tgaccgaagt tttggcgaat aaccttctca tccaaaaaat cggcgtgcag
     8281 gttgcccagg ccgaaaccaa ttcgacactt ctgagtagag aagcttatcc tgacatcagc
     8341 attgccgcct attacgacaa agatacgggc ccttttcagg aataccgatt tggcggcgga
     8401 ctatctttac cgatcccgct ctttaatcga aaccagcacg cggcagcagc agccgatgaa
     8461 tatgcaaagg cagcccgaaa ggaacgggaa tatgctttga gcattctcaa acgggatttc
     8521 aatgcggtat ttgaggagta tttggtgtcg ctccgcctta tcgaaaaatt cccgatcgac
     8581 caggtcgcaa gtacgaaccg caacctgaaa agcctgacgg cagagttcat ccgcagtcgc
     8641 ctcgccactc tgacctatgc cgacgccgaa cagcggatgc aagactcgat tgtatcggct
     8701 tacatggcgc agatggcata cgtaaatgcg gtactgcaaa tagcattatt tcgcggtagt
     8761 gacgatttcg ctgcgtattt ccacaagtaa gaggcatata tgttatctaa aatcgtagaa
     8821 ttttctctca atcaccggct gtcgataatt gttaccctcg tcgccgttct cggcggaggt
     8881 attttcgttg gctttcaatt gccggtcgat gccgtaccgg acatcaccaa tgttcaggtt
     8941 gtggtcaatg ccaaaactgg cggcctcgac ccgcagcagg tggagaaatc cgtcacgtat
     9001 ttcatcgaaa ctgaaattgc cggtatcccg cgagttcagg agatacgctc gctggctcgc
     9061 ttcggtttat cgcaaactgt ggtgattttc aaggacggca cagacatcta ctgggcgcgg
     9121 cagcaagtgg gggaaaaaat gcgtaatgcc tcagaccgct tgcctgctgg agtaagcatg
     9181 gaaatggctc cgatctcgac gggattggga gaagtgctga tgtatgtact cctgcccaaa
     9241 agtggcagcg agttggagaa gaaatccgag ttggagcgat tgctctactt gcgaacaatc
     9301 caggatttcg ttttgcgccc ctacatcaaa tcgaacgcga tcggaattgc ggaggtagat
     9361 agtctcggtg gcttcaaaaa acagatccac atcgattttg aaccagcccg actcgaacaa
     9421 tatggagtta ctttcgacga aattgtccaa aagctcaaga ctgtcggaga gaattttggc
     9481 ggcggttata tcgaaaaaga tacgcagcag tggattgtgc ggacaaaagg acaggtaagc
     9541 ctaaaacaat tcgagcagat tccggtacgc aacaatatat tcggctctcc ggttcgcctt
     9601 aaagaagtgg cgacaatccg cgaagaccat gcgcagagag tcggcgctgc gacctataaa
     9661 ggaaatgagg ctgtgctcgg gactgtgctc atgctcatgg gggagaatag ccgtgaggtg
     9721 gcgctgaacg ccgaacaggt cataaaaaac gcaccgctgc ccgacgatgt cgtcgcggaa
     9781 attgtctata cccggagctt cctcgttaat gcgacgctca aaactgttgc gaagaatctc
     9841 gcggaaggtg ccgcgatcgt catccttatc ctgctgcttt ttctcggaaa tctgcgagcg
     9901 gctcttttcg tctcactttc aattccattg gctatggcct ttgctcttat tggcatgaaa
     9961 gttttccata tttccgcaaa tctaatgagc ctcggtgccc tggatttcgg ccttattgtc
    10021 gatggggcga ttgtgatgat tgaaaatctg ctcgctaaaa tggagcatca gccgccaaaa
    10081 aatatcgccc aaaagctcaa aatggccaaa gacgccgcgc tggaagtttt ggctcccatg
    10141 acgaccggaa tgattattat catgtcggtc tatatcccca ttttgaccct cgaaggcacg
    10201 gaaggcaaac tttactaccc gatggccatg acggtgattt ttgcgctcac gggggcgctc
    10261 ttgatggcca tcgtggcgat tcctgttttg ggatattacg tgcgcgttaa gagcgaaccg
    10321 caggccacga agctttttca ggcggtttac aaggcctatg agccgattct gaattttcca
    10381 ttgcgcaata tccggaagct ggttttacct gttgtcgcat ttttgctcat ctgcggctgg
    10441 atttttgcgc gcttaggttc ggattttatg ccgccgctta acgaagggga catggctctc
    10501 aacctcgtgc acgacgcgaa aatatcgcta agcgaatctc tgacgcgcca gcgcaaaatt
    10561 gaaagcaaac ttgcgactta cccggaaatc gaaagagttt tttcgcgcat tggtacgagt
    10621 gaagccgcta ccgatccaat gggcgtaaat ctttctgatc tgttcgtaat tctgaaaaag
    10681 gatacgtccc agtggcgtaa ggatgctaag ggtaagacgc tcgacaaaga tggcgttttt
    10741 aaactgattg aggctgacgc taaagaactg cttgaaaaaa ctgataagct cgccgaatcc
    10801 gaacttgtgc agacgcaacc cattgcgatg cgtttcaatg agattctcga aggcagtcgc
    10861 gccgatattt cgctgagaat ctacggaagc gatctcgata ccttggcaga actacaggat
    10921 cgctcggtgg agatattgaa aaaaatcaag ggtgcacggg aggtcgagct cgatgcgctg
    10981 acggcattgc gcaaaagtct ggtttttgac gcttcgatta agtacaataa agtagcttat
    11041 tacggcgtct cgctgggaga tgtgaatttc agtctgcaaa cggcgatggc gggtatgacc
    11101 atcggcagtt actatgaaca ggcgtggcag tttccgattg tggtgcggct cagcgaagaa
    11161 aatcgcgaaa aactcagcga aattaaccgt attcccattg cgctaccgac gggaggcacg
    11221 atacccctct cgaaagtcgc ggagttcagt gtcaaagaga acgtcacctc catcgcccgc
    11281 agcgggctgg agcgttatgc tggcgtagcc gtgtttttag gcgaccggga cacattatct
    11341 ttcgttgaag aagcaaaggc caagatcaaa gaagaactaa aaattccgaa aggctatcgc
    11401 ctgagctggg gtggccagtt taagaatctg gaacgagcac gggtgcgact ggcgattatt
    11461 gttccgctta ttttgctggt gattctgttt ctggtgtacc gcaccttcca ctcaattaaa
    11521 caaaccctgt tgatttttct caccataccg ttcgcatgga cggggggtgt cctcgcgctg
    11581 tggtttaccg ggattcactt ctctgtctcc gcggctgtgg gtttcattgc actctccggt
    11641 gttgcggttt tgaacggcct cgttaaggtg agctatctta accagctacg agcgcagggt
    11701 aaatcggtga acgaggcggt acacattggt gcgctggggc gtttgcgccc tgtgttaatg
    11761 accgcttcgg ttgccagcct cggctttctg ccgatggtat taaacacggg catcggagct
    11821 gaggtacaaa ggccattggc aatcgttgtc ctgggcggat tgattacggc aaccctgatg
    11881 acgctactgc tcttgccctc attctatcaa tggatggaaa aaggagatga aaaatgaaac
    11941 aggttttcaa agataagaag tttatttcac tgatcgtagc tattgcgatc gttgtttcgc
    12001 tcgaagtgct ctctttgctt ggcatacata ttccaatgcc ctacgcgccg ttcgttttta
    12061 gtgcgttcat tatcggtatt ggctatggtg taatttggaa aggcttaaag gcgctcgcca
    12121 cgctgcgttt cagcagcatc aatctcctga tgctcatcgc tgttattgga gcgttttatt
    12181 tacgcgagtt tcccgaagcc gcggtcgtga ttgtgctcta cgttctcggc gaacgtctcg
    12241 aagacatcgg tatcgaacag agtaaatccg cacttgacga actggtgagt aaatccccca
    12301 aaactgccgt cgttaaagct atcggggaag ctgttgaaat cgatacgatt ccaatcggta
    12361 caatcattgc cattaaaccg ggcgatctta tacctctcga cgggaaaatc accggcggag
    12421 aaaccgcggt cgatgaagcg gcaatcaccg gcgaacctat tccaaaagac aagcgccccg
    12481 gcgatctggt ttttgctggc acgctcaata aaagcggcta tatagaggtt gagaccacaa
    12541 agctcgccac cgacacgact tttgcaaaga ttattaagct gacttttgag gctcaggcca
    12601 acaaatcgga tacccagaaa ttcatccaga aatttgccag tatttacacg ccgggtatta
    12661 tccttctcgc tactggactc ctcattgttc cggttttttt gttaaagcag gattttagcc
    12721 actggttgca gcaggcaatt acgcttctgg tgattgcgtg cccgtgcgct cttgtgatct
    12781 cgacgcctgt cgctatattt gccgcgatcg gtaacgcttc gaccaaagga gcgcttgtca
    12841 agggcggcaa gttcatcgaa gctcttgcgc gcattcgcgt actggctctc gataaaacgc
    12901 gcaccattac ttttggtaat ccgatagtgt cggatgttat ccctttaaac ggcatttccc
    12961 gtgaagagct tttggcctgc actgccggtg cggaaatatt ttcggaacat ccattggctc
    13021 aagcagtagt cgacgccgcc cgccgcgagg gctttgaacc gcaccgtgcc gaattattca
    13081 aatccatagc gggtaagggt gcaacggcaa aatgtcttgt gtgcgaagac gagacaatct
    13141 atttgggcaa actctcctat attcacgaaa gccaacccag cgacgctgaa gcagagaaca
    13201 tagtcaaacg cctttcaggg caaggaaaaa ccagcgtcgc cgtcagcttt ggtcagggtg
    13261 tcgcaggtat aatcggtctt gccgacgaga ttaaaccgga cagtgctgcg gcaattgatg
    13321 agttgaaggc aatcggcgtt gagcctgtca tgctgaccgg cgacagcgag catgcaggta
    13381 aatatgtcgc cgataaggtt gggattggca gcgtctatgg cagtctcatg cccgacgaca
    13441 aagccgagca gatcaaacga ctcagagcac aatacgaata cgttggtatg gtcggggatg
    13501 gcatcaacga cgctcccgcc ctcgccattt cctctgtcgg tattgctatg ggtgcggcgg
    13561 gcagcgatgc ggcaatcgag tcggcaaata tcgcattgat gaatgacaaa ctttcactga
    13621 ttccatttct cattcggtta agccgcgcgg ctcttttacg gatcaagatc aatacggttg
    13681 cggctgtttt tgttaaactt gcctttgtcg gccnnnnnnn nnnagtaatc ttgtgtttgc
    13741 gattgccgct gatgtggggg ttaccctggt ggtaattatt tttagtttga ggctattgaa
    13801 atttggcggc gccgagaggt gattccggag gaaaaatgaa tgagatacta attcgcgaaa
    13861 ctcccatctt ggatttcaac cacccatcgt tgaaagcgct catcgaccgc tgcggctgga
    13921 aggcattgtc tgaaagagac cgcatcctcg caatttacaa cttcgtgcgc gacgaaatcc
    13981 aattcgggta caatgtatca gacgatatcc ctgcgtcagc tgtgctggcc gatggttatg
    14041 gccagtgcaa tactaaaggt aatctcttca tggcgctatt gagggctgtc ggtattccct
    14101 gccgatttca cgggtttacg atcaataagg aattacaaaa aggagcaatt acgggaatct
    14161 ggtatcgcct tgctccgcag gaaatcgttc atagttgggt agaaattctt tttgaaggca
    14221 aatggcgaaa catagagggt ttcattctcg ataagcagta cctgaccgcc ttacaggtgc
    14281 gtaacccgga agtcagagga tatttctgcg gttacggcgt tgccaccgat aattttgcca
    14341 atccgcaggt agattggaac ggtggagata cgtacatcca gaaggaaggt ataaagcagg
    14401 attttggaat tttcgatagc ccggatgatt tttatagagg ccaccggcag aaattgaatt
    14461 ggctaaagcg tttgattttt cgctggctaa ccaggcattc catgaacagg aacgtgtcac
    14521 ggattcgtcg aaattaatat ccagaggtga aaaaatccaa tattctccta aatattgtca
    14581 atctgccttc gccctccgtg gctcggcagc gtcgtcgcta ggatgcgagg cacgtcgcct
    14641 aacaaaattt acctgctgcg ctcgctgcgg tg
//
LOCUS       DAOMPD010000007        14101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1857_length_14101_cov_2.715293, whole genome shotgun sequence.
ACCESSION   DAOMPD010000007 DAOMPD010000000
VERSION     DAOMPD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14101
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_14101_cov_2.715293"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /gene="ftsH"
                     /locus_tag="PLY93_00560"
     CDS             complement(<1..1436)
                     /gene="ftsH"
                     /locus_tag="PLY93_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRP68010.1"
                     /translation="MTPQKQGPLGAPTPNKTMKNIALILLIVIMAALTYEVFQEQQAK
                     VTEIHYSKFLNLVDTESLSVTEQAPLVIQGNVVSGKYFDKEKKADVLFKTVIPYVDDK
                     LLDNLKAHKVKFFKGAPVEESFFWRTLMPLLPWIAIVAFFWFMMARQVQSTGNRALSF
                     GKSRPKVGPDMKNRITFADVAGVEEAKEELKEVVDFLKAPQKFQAIGAKIPRGVLLVG
                     PPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFGQAKRQTPCIVFI
                     DEIDAVGRLRGAGLGGGHDEREQTLNQLLVEMDGFEENEGVIIIAATNRADVLDPALL
                     RPGRFDRQVIVDVPDVKGREAILKIHSKKVPIEEGTTMDKIARGTPGFTGADLANLIN
                     EAALLAARNDRKKVSEHDLDDARDKVLMGPERRSFLIIPEEKKIIAYHEAGHAILGEL
                     LEHSEEIHKVTIIPRGRALGITQHLPEQ"
     gene            1658..1906
                     /locus_tag="PLY93_00565"
     CDS             1658..1906
                     /locus_tag="PLY93_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68011.1"
                     /translation="MSTMKEELKEKAKIKLYLGHDEDEDELDDDDESEDESEREIAVS
                     YACEDCDYRWEIYIEEEDDEMDDTQYCPMCGSANTSQI"
     gene            complement(2258..3184)
                     /gene="htpX"
                     /locus_tag="PLY93_00570"
     CDS             complement(2258..3184)
                     /gene="htpX"
                     /locus_tag="PLY93_00570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRP68012.1"
                     /translation="MGKRILLFIVTNILVVITISILINLLGVRYYIENTASGYGLNLQ
                     ALLIFCLIWGFVGAFISLALSKVMAKMMMGLSVIDPRNASGIEAQLVATVHRLAKAAG
                     LTQMPEVAMYESEEVNAFATGPTRSSSLVAVSSGLLRKMEWSEVEGVLGHEVAHIANG
                     DMVTMALLQGVVNAFVMFLSRVVAFAISTAMSRSDDREGASFPSLLNFILTIVFDIIF
                     TLLGSLVVAAFSRYREYRADAGGASLAGSSKMIAALERLKSTQELVDTNTAPSMASMK
                     ISNKKGFLALFSTHPPLKDRIQRLQNSAAGWV"
     gene            3289..4635
                     /locus_tag="PLY93_00575"
     CDS             3289..4635
                     /locus_tag="PLY93_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRP68013.1"
                     /translation="MDIYTDRIRRVRARMQENSILLIKAAPPVIRNNDTYYPYRAHSD
                     ILYLTGVNEEELSLLITQDAVYVFARVRDAERERWTGFVKGHAFYQQLFSALGDKNIF
                     LHETKDFSKIFSDQILSKQILYFDFGIDPALDAQILGVLQNARRFARRGVQVPPTITQ
                     ASTILSECRLFKDAHDLLCMREAARISSEAHNKTIEFIESWRGNTLDEFCVKALIEHE
                     FMKLGADRLAYPSIVAGGKNATILHYENTEGTAPKGAFILIDAGCEYKGYASDITRTT
                     VTGGKNFESALQKELYALTLKAQTEAIRQTKAGSTLDSVHDAAVDVLARGLLEMGFFK
                     SVPLRKKDEKVDTQTLISVTSIDEIKELEYYNLFYMHRTSHYLGLDVHDVGSYYHNGK
                     SRALENGMVITVEPGLYFPEEYDFIPAEARGIGIRIEDDVLVTQEGNEVLTQACRK"
     gene            4641..5420
                     /locus_tag="PLY93_00580"
     CDS             4641..5420
                     /locus_tag="PLY93_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68014.1"
                     /translation="MFWRRKCSLVVLCCICISTEILSSETITPNKENETKEVKPEPNP
                     QIQFEDWQSPLSEENTERHLVTMAVNPTPKIVEATKEVYPIYVGVALVLAKHLEEGVD
                     QSKGDYHVGYTAFGGALSGEYEYHAFLFQGAFTFMRLFQMNVNGYSIDMRERTQWHLL
                     FSANMFYSIYPSMALGVGLTHLTETSMYVNDTKVADSSFQHIFLDIAARYMPRLTDKM
                     NLDIAAVVGLNMLPGRRHTYSIFDLVHARFQINVGVRYRVF"
     gene            5420..6241
                     /locus_tag="PLY93_00585"
     CDS             5420..6241
                     /locus_tag="PLY93_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRP68015.1"
                     /translation="MKTVLLRILALGVLATLLIFFWDLPLLKPFKLFVVFLHETSHAM
                     AALLTGGRLSAISIAWDESGATYTSSGEGIFFVIAIAGYIGSILWGQAMLRASLSGRY
                     VRTVSVVVGLTVIFFGFFPDGDGVAKNSDKLLKYGISGIWGLSLILSAFLLPRFNHVL
                     LFFLGGLTALYSLYDLNDFIHGDVMRTDAGILAHYLLGSSFLVKPMAYIIAGFISAIS
                     IYIFWRLVRSTFHVEEEYDDEKVDLAEWQEQNPDIEITPEMLAWLKDKAKHNSRE"
     gene            6276..7415
                     /locus_tag="PLY93_00590"
     CDS             6276..7415
                     /locus_tag="PLY93_00590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRP68016.1"
                     /translation="MRVSSGIQSELDLINQVFANDDADEEEIIAAADILFKSGEKNIY
                     ARLLHVMCHLRLSPSDARKTWQSILKHRDILEEKLERHVGFRVAMLDYFVNESHELKN
                     PKVIEIRVYAETARQAAVDELTGLFNRRFFDTAIERELKQAARRHRQFSLLILDIDNF
                     KKINDTHGHTTGDAVISALGKLLKKLIRSEDTPCRIGGEEFAIIFPETAADGAMTVSE
                     KIRREFSKLKIAATDLSLCGGLTTYPDDGTTVTELIDRADQMLYFAKYSGKNRVICYS
                     QNKRRHIRYPLEWEIAITQKDQTSSKGKSLDVSIEGISFELDVKTEPGEKFNLWIDID
                     HNPVELPVEIVWVDNKAKSRKLRAGARFLALKARDLTTLKEHLPH"
     gene            complement(7566..8378)
                     /locus_tag="PLY93_00595"
     CDS             complement(7566..8378)
                     /locus_tag="PLY93_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP68017.1"
                     /translation="MHSSKKMILPPKQIQRDASKTQKIIRLLLSLSLLVLSARCISSQ
                     TTDNGTHRITLEEFNTLDWQQQVETIEKMNTSFFTDEDKKIIASALLDDDGAVIVAAL
                     EAVWRLKISDFLSQAVVLVDSPDPIIRWRSLLAIEKLGFDDTYRDLVAKAINDKEWMV
                     RETAYRTLRNFKKEHNEKKYFYSVLFKLNEQNPQVVAEIYRTLVWYKDTAAYPYMIKR
                     SYHCKSVSELILVMRELARTQTRDAQVRLKTLTRSQSAMVRTEADKLLDEYY"
     gene            8437..9075
                     /locus_tag="PLY93_00600"
     CDS             8437..9075
                     /locus_tag="PLY93_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68018.1"
                     /translation="MSIQTPQFIDRLERKYYKLGIENLGLILVIMQAFGFLAFQISPD
                     AGMKFALIPQLLLAGEIWRAITFIALPLSTGFWIIIVLFFLYWIMQIIEQAWGPFKTT
                     LYFLMGLIFSIIYSIATGYPITTFMPLEMSLFFAVAALYPTTQVLLFFIIPVPLWILA
                     AFQAVLVIIIMLSSDWLTRGYYAVVYLNYFLFFGLHHYKQLQQRMRRNTWRG"
     gene            9080..9664
                     /locus_tag="PLY93_00605"
     CDS             9080..9664
                     /locus_tag="PLY93_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRP68019.1"
                     /translation="MRVVKYILLFGLITHALSARTEWYDIDYAKSQITFLAKSRLVRA
                     NGIFRKWNFAGKIGASFYVVGDALIDCVSIDTDNERRDNHLKSVDFFDCAKYPQHVFR
                     VRSVKPNNKNSLKATHFEITGDLTMHGVTQPIALTLHREGKDEKPSLVGSFILNRDNF
                     GITYNSVLNPIEKEVRIELKIYLLRRTTKGPNGS"
     gene            9675..>9900
                     /locus_tag="PLY93_00610"
     CDS             9675..>9900
                     /locus_tag="PLY93_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68020.1"
                     /translation="MRIIISLLIVFAVVAFWVLRPIRDTDFSLVGFIPLRKDALAARF
                     APLILPHEMYGTATRLYYRMAKDDEGKIYIA"
     assembly_gap    9901..9910
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9911..10362
                     /locus_tag="PLY93_00615"
     CDS             <9911..10362
                     /locus_tag="PLY93_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68021.1"
                     /translation="HPYFEDEMNPHKGFGATMSRIIYTGGLRLKDFIFGKADLELIEV
                     VLDKNLTPLELSWEGVENYQPKNFGVKHKNQTEKNAKPPYCFSVITWNHMVARTDASA
                     CMAAPRLTPQYFAEEAWQEFRMVKKTEAILRRNRAHRAYERKAASTR"
     gene            complement(10372..11046)
                     /locus_tag="PLY93_00620"
     CDS             complement(10372..11046)
                     /locus_tag="PLY93_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68022.1"
                     /translation="MKKILSTIFFVLSVGTLFADSARPEILDRIRDYDLRHTDLLVKI
                     VRDSETLRYAKVRALEKIAILYRQSKTEAEAVTPRLFEGIQAGLAHKAPDVREAACNA
                     SVVFNESKIAPLLTNALAKTLKEETQPTVLYACAHTMRVFTKDTEAAVLAPALLAQVN
                     RYLVTLLDDSKNEKALREICLALGAAKDRKSFISLLKILQSRYDESVKDAAQEALQAI
                     RVPERK"
     gene            complement(11043..11639)
                     /locus_tag="PLY93_00625"
     CDS             complement(11043..11639)
                     /locus_tag="PLY93_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68023.1"
                     /translation="MASENQGESKKPHFWQRKYFIDKRFQSKFMLRYSMIVAVSAILI
                     LGGLFALKNKAYSLLPDGASVLAQMDVDNHKNLEKEADGKWVVVPEGKGEEYFPLKVT
                     QGGYKFYNAFNLYIWPVVVVTILNILIILIYSLFFSHKMAGPVFKIKKILNQYLATGE
                     FTDIKLRNGDNFDDLARLINRAIRRRENKPDTENNVGE"
     gene            complement(11672..12427)
                     /locus_tag="PLY93_00630"
     CDS             complement(11672..12427)
                     /locus_tag="PLY93_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68024.1"
                     /translation="MRLGVVNFLNAWPLWGALENSSAVTLVPDIPSRLVAYLREGKVD
                     AALISSIEFFRLGKGYTYHKGVCVGAKNRVASIRFFTADASLSFRETLTRLNIIYTDA
                     ASRSSVAQLKVILKELGINIPFQEIDNADEKIPQLAKGEGLIAIGDTALAHRTRLSFD
                     VQQEYYSLFKRGFVYALWAYPTALQNSLDTLFTTAYKNYLANEEQLLKEAIRRFGFDA
                     DFTQEYLTKIIQHTLTPERANDLFFFVEKHKNI"
     gene            12512..>14101
                     /gene="ligA"
                     /locus_tag="PLY93_00635"
     CDS             12512..>14101
                     /gene="ligA"
                     /locus_tag="PLY93_00635"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRP68025.1"
                     /translation="MTKKNFKAKEKMTRADYQALIDTIYHHNRKYYRDDAPEISDADY
                     DELMRLLQELEAKHPDWQQSDSPTLKVGGEVREDFAAVKHDPPMMSLDNAMDVGELKA
                     FHERLVKAGLKNPTYHVEPKFDGLAIELLYIDGKLAVGSTRGNGDVGEDITHNIRTVA
                     NVPLRLLCENPPELLSVRGEVIIKLADFLKLNERQEREGKKLFANPRNTAAGALRQQD
                     SKEAAGKSLSFFPYSLGKIVDAKNRFAKALRSQQKIWQEVFVELGFQIAAYHKKTNLA
                     GIEAYYQEMQEERASLPYDLDGLVVKLDDTSEWKDFGATTKAPRWAIALKFPPRVGFT
                     RLQNVAYQVGRTGVVTPVAELLPINLGGVLVSRASLHNADEIERLDLHEGDSVEVVRS
                     GDVIPKVVRVFPEKRAQNARPIEFVAACPACGRKLMREDVFYRCVNPTCEGINEAALQ
                     FFVSKGGIDVEFLGQEWVAKLYKAGVIQDFADFFRLTQIDLLTFEGMGEILAAKILKS
                     IAARRTIPLAKFLTAIGIANVG"
BASE COUNT         3701 a         3197 c         3361 g         3832 t
ORIGIN      
        1 tcttgttcgg gcaagtgttg tgtgatcccc agcgcacgac cacgcggaat aatggtgact
       61 ttgtggattt cttccgaatg ctctaaaagc tcacccaaga ttgcatggcc tgcttcatgg
      121 tacgcgataa tttttttctc ttcggggata atgagaaacg atctccgttc tggccccatg
      181 agcactttgt cgcgcgcatc gtccaagtcg tgctcagaaa cttttttgcg gtcattccgc
      241 gcggctaaaa gtgcggcttc gttaatgagg ttggctaaat cggcgccggt aaagccaggg
      301 gtgcctcggg caattttatc catcgtggtg ccttcttcga tgggcacttt tttgctgtga
      361 attttcaaaa ttgcttcgcg ccctttcaca tcgggtacgt cgacaatcac ttgtctgtca
      421 aagcggccag ggcgcaaaag ggcagggtcg agcacgtcag cgcggttggt ggcagcgata
      481 atgataacgc cttcgttttc ttcaaatcca tccatttcaa caagaagttg attgagcgtt
      541 tgctcgcgct cgtcgtggcc accgcctaaa ccagcgccac gcaagcggcc cacagcatcg
      601 atttcgtcaa tgaaaacgat gcatggagtt tggcgtttcg cttgcccaaa aagatcgcgc
      661 acacggcttg cgccgacacc cacaaacatc tccacaaagt cggaacctga gatggagaag
      721 aaaggcactc cagcttcgcc cgcaacggct tttgccaaga gcgtttttcc cgtgcctgga
      781 gggccaacga ggagaacgcc acggggaatt tttgcgccaa tcgcttggaa cttttgtggg
      841 gctttaagga aatccaccac ttctttgagc tcctctttgg cttcttcgac accagcaacg
      901 tcggcaaacg tgatacggtt tttcatgtcc ggccccactt taggccgtga ttttccaaat
      961 gaaagcgcgc ggttgccggt cgactgtacc tggcgggcca tcatgaacca aaaaaatgcc
     1021 acgatcgcaa tccacggtaa aagcggcatg agggtgcgcc agaaaaaact ttcttcgacg
     1081 ggggcgcctt tgaagaattt tactttgtgc gcttttaagt tatcgaggag tttatcgtcg
     1141 acgtatggaa taactgtctt aaaaaggaca tccgcttttt tttctttatc aaagtattta
     1201 cctgaaacga cattcccttg aatcacaaga ggggcttgtt cggtgacgga gagagactcg
     1261 gtatcgacga gatttaagaa ctttgaataa tgaatttctg taacttttgc ctgttgctct
     1321 tggaatacct cgtaggtgag ggcggccatg atgacaatga gtaatatgag tgcgatattt
     1381 ttcattgttt tattgggcgt cggcgcgccc aaaggccctt gcttttgtgg tgtcatgcga
     1441 tatcatctcc ataccaaaat tgataaaaac cgatacagtt tttttgacaa gcggtcaata
     1501 gggattacct tcttcaaaat atgcgcaacg cttgcggcat atcttttgaa gaagccttta
     1561 ttgacgaaca agtctataat cagaagaata ctaaaaagtg gataaaatgt caaaaatact
     1621 tgaccggtgt tatcgaaaat gaggcacgcg ttcaagcatg tcaacaatga aagaagaact
     1681 taaagaaaag gcaaaaataa agctttattt agggcacgat gaagacgagg acgaactcga
     1741 cgacgacgat gaatcggaag acgaaagcga gcgcgaaatt gcagtgagct acgcatgtga
     1801 agattgcgat tatcgttggg aaatttacat cgaagaagaa gacgatgaaa tggacgatac
     1861 gcaatactgc cccatgtgtg gtagcgccaa cacgagccaa atatagcgat gcagcgcctt
     1921 aatgttttgg cgcttttatt tatccttttg gtgcttttgt tattggtgaa tagccagaag
     1981 gtgcagattc acatcttctt tgtgcaaaca atattgcctt taggcggtat catggcggcg
     2041 tgtgttgcct ttggcgtcgt attgacgctt ttgtttttta gcctgggacg ttcgtataag
     2101 aaattagtgg cgcgacttaa aaatatttat agatgagggt atctaaaaat tcgaaatttt
     2161 tatattgatt ggggggcagt cccaatacaa taaattttaa cttttaggct gagtaatagg
     2221 cgcgccaaca actgattatc cagccccctt tattctatta cacccaccct gccgcagagt
     2281 tctgtaaacg ttggatacgg tctttgagcg gtgggtgcgt cgagaaaaga gcaagaaatc
     2341 cctttttatt tgatattttc attgatgcca tcgacggtgc ggtgttggta tcgacgagtt
     2401 cttgcgtgct tttcaaacgc tctaaagcag cgatcatttt ggaactcccc gcaagcgagg
     2461 cgccacccgc atcggcgcgg tattcgcggt aacgcgaaaa cgccgctacg acgagcgaac
     2521 ccaagagagt aaaaataata tcgaaaacaa tggtaagaat aaaatttaag agcgatggaa
     2581 aagatgcccc ttcgcggtcg tcgctacgac tcatcgctgt gcttatggcg aaggcaacca
     2641 cgcgagacaa gaacatgacg aaagcgttga caacgccttg taaaagagcc atcgttacca
     2701 tgtcgccgtt tgcaatgtgc gccacttcgt ggcccaacac cccttcgact tcgctccact
     2761 ccattttacg caaaaggccg cttgagactg cgacgagaga actggagcgg gtgggcccgg
     2821 tggcgaacgc attgacttct tctgattcat acatcgccac ctcgggcatt tgggtgaggc
     2881 cagcggcttt agcaagacgg tgtacggtgg ccaccagttg tgcttcaatg ccggaagcgt
     2941 tgcgtggatc gatgacggaa agccccatca tcattttggc cattactttt gagagtgcga
     3001 gtgaaataaa cgcacccaca aaaccccaga tgagacagaa aatgagcaat gcttgtagat
     3061 tgagcccata acccgaagcg gtgttctcga tgtaatagcg aacacctaac aggttaatca
     3121 aaatggaaat cgtgatcact accaaaatat tggtgacgat gaaaagaagt attcttttac
     3181 ccattcgtat aatttatcat aaagcaaatg tattggccaa ttttcacgaa tttttacacc
     3241 ccgaaaccgc tttacgggcg atttttgcat cacttatcgg catagttcat ggatatttat
     3301 accgaccgca tccgccgtgt acgcgcacgg atgcaagaaa atagcattct tctcattaaa
     3361 gcggcacccc ccgtaatacg taataacgat acgtattacc cttaccgtgc gcattctgat
     3421 attctctatt taaccggcgt taacgaagag gagttatctc tcctcataac tcaagacgcg
     3481 gtgtatgtct ttgcgcgtgt gcgcgatgcc gagcgcgaac gttggacagg ctttgtgaaa
     3541 ggccatgcgt tttaccaaca gcttttttcg gcgttagggg ataaaaatat tttcctccac
     3601 gaaacaaagg atttttcaaa gatctttagc gatcaaattt tatcaaaaca aatcctttat
     3661 tttgattttg gcatcgatcc ggctttggat gcacaaattt taggggtgtt acaaaacgcg
     3721 cgccgctttg cccgccgtgg cgtccaagtg ccgccaacca tcactcaagc atcgacgatt
     3781 ctcagcgaat gccgtctttt caaagacgcg cacgatcttc tttgcatgcg cgaggcagcg
     3841 cggatttcct cggaggcgca caacaaaacc atcgagttta ttgaatcgtg gcgcggcaac
     3901 accctcgatg agttctgtgt gaaagcgctc atcgagcatg agtttatgaa attgggcgca
     3961 gatcgtttag cgtatccatc gattgtcgct gggggtaaaa atgccacaat tttgcactat
     4021 gaaaacactg aaggtaccgc tcccaaggga gcctttatct taatcgatgc ggggtgcgag
     4081 tataagggct atgcctctga catcacgcgc acaacggtca ccggtggaaa aaattttgag
     4141 agtgcgcttc aaaaggaact ttacgcgctc acgctcaagg cgcaaacaga agcaatccga
     4201 caaacgaaag cgggtagtac cctcgatagc gttcatgatg cggcggtcga tgtgttggcg
     4261 cgcggcttac tcgagatggg tttctttaag agcgtgccgt tgcgcaaaaa agacgagaag
     4321 gtcgacaccc aaactttaat ctccgttacg tcgatcgacg aaataaaaga actcgaatat
     4381 tataatttat tttatatgca ccgcacgtcg cattacttgg ggcttgatgt gcacgatgtg
     4441 gggagttatt accacaacgg caaaagccgc gctctggaaa atggcatggt catcaccgtc
     4501 gaaccggggc tttatttccc cgaagagtac gattttatac ccgctgaggc gcgcggtatc
     4561 ggcatccgca tcgaagacga tgtgctcgtc acccaagagg gcaacgaagt tttgacgcag
     4621 gcatgccgaa aataaataag atgttttgga ggagaaagtg ctctcttgta gtgttgtgtt
     4681 gtatttgcat ttccacagaa attctttcaa gtgaaacaat aactccaaac aaagaaaacg
     4741 aaacgaaaga agtcaaacca gaaccgaacc cccaaataca atttgaagat tggcaaagcc
     4801 ctctttctga agaaaatacg gagcggcatc ttgtaacgat ggcagtgaat ccaactccga
     4861 aaattgtaga agcaactaaa gaagtgtatc ctatttatgt gggcgttgca ttggtccttg
     4921 ctaaacatct tgaagaaggc gtcgatcaat cgaaaggaga ttatcacgtc ggttacactg
     4981 ccttcggtgg cgcactatcg ggtgaatacg agtaccacgc gtttcttttc caaggcgcct
     5041 ttacgtttat gcgacttttt caaatgaacg tcaatgggta tagcatcgat atgcgagaac
     5101 gcacgcagtg gcatctttta ttttcagcga atatgtttta cagcatttat ccatcgatgg
     5161 cgttaggtgt gggacttacg cacctcaccg aaacgagtat gtacgtgaac gatactaaag
     5221 tcgccgactc cagctttcaa catatttttt tagacatcgc cgctcgttat atgcctcgcc
     5281 tgaccgacaa aatgaattta gacatcgctg cggtggtggg gctcaacatg ctccctggca
     5341 ggcggcacac gtattctatt tttgatcttg tacatgcacg ctttcaaata aatgtaggag
     5401 tacgctaccg cgttttttga tgaagacagt tttgttacgt attttggctt tgggcgtttt
     5461 ggcaacgctg ttgatttttt tctgggatct ccctcttttg aagccattta aactcttcgt
     5521 tgtttttctc cacgaaacct cgcacgcgat ggctgcgctc ctcaccggcg ggcggctttc
     5581 ggcaatttcg attgcgtggg atgaatcggg ggcgacatac acttccagcg gtgaaggtat
     5641 cttttttgtt atagcaatcg ccggttatat cggcagtatt ttgtgggggc aggcgatgct
     5701 gcgcgcgtcg ctttcagggc gttatgtgcg cacagtgtcg gtcgtggtcg ggctcaccgt
     5761 tatttttttt ggctttttcc ccgacggcga tggggtggca aaaaattcgg ataaactctt
     5821 gaagtacggc atcagtggta tttgggggtt atcgctcatc ctttctgcgt ttttactccc
     5881 tcggtttaat catgttttgc ttttcttttt aggtggcctt actgcgcttt attcgctcta
     5941 cgacttgaac gattttatcc acggcgatgt gatgcgcacc gacgcgggca ttttagcgca
     6001 ctatctttta gggagttcgt ttctcgtaaa accgatggcg tatatcatcg ccggttttat
     6061 tagcgccatt tcgatttata ttttttggcg tttggtgcgc tccacattcc atgttgaaga
     6121 agaatacgac gatgaaaaag tggatttagc cgaatggcaa gaacaaaatc cagacattga
     6181 gattactcct gagatgctcg catggctcaa ggataaagcc aaacataatt cgcgggaata
     6241 actttacatc acgtaaatca atcctacggt atgccatgcg cgtttcgtct ggaatacaaa
     6301 gtgaattgga tttaatcaac caagtgtttg cgaacgacga tgccgacgaa gaagagatca
     6361 tcgccgccgc cgatattctt tttaagtcgg gagaaaaaaa tatttacgct cggcttttgc
     6421 acgtcatgtg tcacttgcgg ctcagcccca gcgatgcacg taaaacatgg cagagcattc
     6481 tcaagcaccg cgacatcctt gaagaaaaac tcgagcgaca cgttggtttt cgggtggcga
     6541 tgctcgacta ttttgtgaac gaaagccacg aactcaaaaa ccccaaagtc atcgaaatcc
     6601 gagtgtatgc cgaaaccgcg cggcaggctg cggtcgacga actcacaggt ttattcaacc
     6661 gccgtttttt tgataccgcc atcgagcgcg agttgaaaca agcggcacgc cgccaccggc
     6721 agttctcact cctcatcctc gacatcgaca atttcaaaaa aattaacgac acgcacggcc
     6781 acaccaccgg cgacgccgtg atttcggcac tgggtaaact cttaaaaaaa ttgatccgca
     6841 gcgaagacac gccgtgccgc atcggaggcg aagagtttgc cattattttt cccgaaaccg
     6901 ccgccgacgg tgcgatgacg gtgagtgaaa aaattcgacg tgaattttca aagctcaaaa
     6961 tcgccgccac cgatttgtcg ctctgcggcg gactcaccac gtatcccgac gacggcacga
     7021 cggtaactga acttatcgac cgcgccgatc aaatgcttta ttttgcaaaa tactccggca
     7081 agaaccgtgt catctgctat tcgcaaaata aacgccgcca catccgctat cctctggagt
     7141 gggagattgc catcactcaa aaagatcaaa cttcatccaa aggaaaatcg ctcgatgttt
     7201 caatcgaagg gatttcgttt gaacttgacg ttaaaacaga accaggggaa aagtttaatc
     7261 tatggatcga tatcgaccac aaccctgtcg aattacccgt tgaaattgtt tgggtggaca
     7321 acaaagccaa gtcacgcaaa ttacgtgcgg gtgcgcgttt tcttgccctc aaagcgcgcg
     7381 atttaacgac actcaaagag catttaccgc attagcgggt tttgggtttt tgagtctgtg
     7441 cgccaactcg ctgagacaat tttttttatg ctgtttttgt tgatatatgt agcgctgttt
     7501 aagtctactt ttgattgcct taaatcatct atggtaagaa tgtaaccaat ttcgattctt
     7561 tgaacttagt aatactcgtc taacagcttg tctgcttcgg tacgcaccat tgcgctttga
     7621 ctgcgggtga gagtttttaa gcgtacttgc gcatcacgag tttgggtacg cgctagttcg
     7681 cgcattacta aaatgagctc cgataccgac ttgcaatggt aactgcgctt aatcatataa
     7741 gggtatgcgg cagtgtcttt ataccaaacc aaagtgcgat aaatttcagc gacgacttgc
     7801 gggttttgct cgttgagttt gaagagcacc gaatagaaat attttttttc attatgctct
     7861 tttttgaagt tgcgtaatgt ccggtaagcg gtttcgcgca ccatccactc tttgtcgttg
     7921 attgctttgg caacaagatc tcggtaggtg tcgtcaaaac ctaatttttc gatggctaaa
     7981 agtgagcgcc agcggataat tggatcgggg ctatctacaa gaacgactgc ttgcgataaa
     8041 aagtcgctga tcttgagacg ccacactgct tcgagcgctg cgacgataac tgcgccatcg
     8101 tcgtcgagaa gagcggaggc aataattttt ttgtcttcgt cggtaaaaaa tgacgtattc
     8161 attttttcga tggtttcaac ttgttgctgc caatccaatg tattaaattc ttcaagggta
     8221 attcggtgtg ttccgttatc cgttgtttgt gacgatatac atcttgcaga aagaacaagt
     8281 agggatagcg ataaaagaag tcgtataatc ttttgtgtct ttgaagcgtc tctttgtatc
     8341 tgtttagggg ggaggatcat ttttttagaa gaatgcatgg atttttaata cattacaata
     8401 tcgattggaa attattgttc gtatttaata aaccctatgt caattcaaac accacaattt
     8461 atagaccggc ttgagcgcaa gtactacaaa ctcggcattg agaatttagg acttattttg
     8521 gtcatcatgc aagcgtttgg ttttcttgca ttccaaattt cacccgatgc ggggatgaaa
     8581 tttgcgctca ttccacaact tttgctcgcc ggtgaaattt ggcgtgccat cacttttatt
     8641 gcgcttcctt tgagcactgg tttttggata atcattgttc tcttttttct ttattggatt
     8701 atgcaaatca ttgagcaagc atggggacca ttcaaaacga cgctttattt tttaatggga
     8761 ttaatttttt cgattattta ctccatcgcc acgggatatc ccataaccac attcatgcca
     8821 ttggaaatgt ctcttttttt tgccgttgcc gcgctctatc ctacaacgca agtgcttctt
     8881 ttctttatta tccctgtgcc gctgtggatt ttggcggcgt tccaagccgt tcttgttatc
     8941 atcattatgc tctcgagcga ttggcttaca cgagggtatt atgcggtggt gtacctcaat
     9001 tattttctct tttttgggtt acatcattac aaacaattac agcaacgtat gcgtagaaac
     9061 acttggcgtg gttaaaccta tgcgagtcgt aaaatatatt ttactcttcg gattaattac
     9121 acacgcgtta tcggcgcgca ccgaatggta cgatatagat tatgcaaaat cacagattac
     9181 atttttggca aaatcgcgcc ttgtacgagc aaatgggatt tttcgtaagt ggaattttgc
     9241 cggtaaaatc ggcgcgagtt tttatgttgt gggcgacgct ctcatcgatt gcgtctctat
     9301 cgataccgat aacgaacgcc gcgataatca tttgaagagc gtcgattttt ttgactgtgc
     9361 aaaataccca cagcatgtgt tccgtgtccg ctcggtcaaa cctaataata aaaattcact
     9421 taaagcaacg cattttgaaa taacgggcga tttaacaatg catggcgtta ctcaacctat
     9481 agcgctcacg ttacaccgtg aaggcaaaga tgaaaaaccc agccttgtcg gtagttttat
     9541 cttaaatcgc gataattttg gcatcactta caatagtgta cttaacccta tcgaaaaaga
     9601 agttcgtatt gaactaaaaa tttatctctt gcggcgcacg accaaaggcc ccaatggttc
     9661 ttaagcatcg cttattgcga ataattatct cgttactaat tgtctttgcc gttgttgcgt
     9721 tttgggtatt gcgccccatt cgggacaccg atttttcatt agtgggattc ataccgctgc
     9781 gtaaggacgc tctggcagca cggtttgcac cgcttatttt accacacgaa atgtacggca
     9841 cggcaacgcg tctttattat cgtatggcga aagacgatga gggaaaaatt tatattgcct
     9901 nnnnnnnnnn accatcctta ttttgaagac gaaatgaatc cgcacaaagg ttttggcgca
     9961 acgatgagcc gtatcattta cacgggtggt ttgcgtctca aagattttat ttttggtaag
    10021 gcagaccttg aacttattga agtggtgtta gataaaaact taactccgct tgaactttcg
    10081 tgggaggggg ttgaaaatta ccaacccaaa aattttggag tgaaacacaa aaaccaaacc
    10141 gagaagaacg caaagccacc gtattgcttt tcggtaatca cttggaatca catggttgcg
    10201 cgcacagacg ctagcgcctg catggcggcg cctcgactta caccacaata ctttgcggaa
    10261 gaggcatggc aggaatttcg tatggtaaaa aaaaccgagg cgattttacg gcgcaaccgt
    10321 gcacaccgcg catacgaacg aaaagcggca tcgacgcgct aagtcagcgc tttactttct
    10381 ttcgggaacg cggatggctt gcaatgcttc ttgtgcggcg tctttgacgc tctcgtcgta
    10441 gcgcgactgc aatattttaa gcaaagagat aaaggattta cgatcttttg ccgcccctaa
    10501 tgcaaggcat atttcgcgta atgctttttc gttcttgctg tcgtctaaga gtgtcactaa
    10561 gtaacgattc acctgcgcaa gtaacgcggg cgctaaaaca gcggcttcag tatctttagt
    10621 gaaaacacgc attgtgtgcg cacaggcata gaggacggtg ggttgtgttt cttctttaag
    10681 ggtttttgcc aacgcattcg tcaatagtgg ggcaattttg gattcattaa aaacaacact
    10741 ggcattgcat gctgcctcgc gtacatccgg tgctttatgc gcaagaccag cctgaatgcc
    10801 ttcgaacagg cgtggagtga ccgcttcagc ttcggttttt gattggcggt ataaaatagc
    10861 aatcttttca agcgcgcgta cttttgcata gcgtaacgtt tcgctgtcgc gcacaatttt
    10921 gaccagaaga tcggtgtgcc gcaaatcgta atcgcggatc cggtctaaaa tttcagggcg
    10981 cgctgagtcg gcgaaaagtg tacctacact caatacaaaa aatattgtgg ataaaatttt
    11041 tttcattcgc ctacgttatt ttctgtatcg ggtttgtttt cacgccgtcg tattgctcgg
    11101 ttgatgagcc gcgctagatc gtcaaaatta tcgccgttac gtaatttaat atcggtaaat
    11161 tcgcccgtcg ctaagtattg atttaagatt tttttaattt tgaaaactgg gcctgccatt
    11221 ttatgcgaaa agaaaagact ataaatgaga ataatgagga tgttgagtat agttacaacc
    11281 acaacaggcc aaatatacaa attaaatgca ttataaaatt tatatccgcc ttgggtcact
    11341 ttcaacggaa aatattcttc gcctttaccc tcgggtacaa ccacccactt accgtcggct
    11401 tctttttcca agttcttgtg gttgtcgaca tccatttggg cgagcaccga tgcaccatcg
    11461 ggcaaaagac tgtatgcttt gttctttaat gcaaacaatc caccaagaat gagaattgct
    11521 gaaaccgcga caatcataga gtatcgtagc atgaacttac tttgaaatcg tttgtcgata
    11581 aagtatttac gttgccaaaa atgcggtttt ttactttcgc cttgattttc cgatgccatt
    11641 agggtacagg tctaagtcac aaacaagccg gtcaaatgtt tttgtgtttc tcgacaaaaa
    11701 aaaataaatc gttggcgcgc tcgggtgtga gtgtgtgctg gataattttc gttaagtatt
    11761 cttgagtgaa atcggcatca aatccaaacc ggcggatggc ttctttaagc agttgttctt
    11821 cattcgctaa ataattttta tatgcagtcg tgaataacgt atccagggaa ttttgtaaag
    11881 cagtcggata tgcccagaga gcatacacaa acccacgctt gaaaaggctg tagtattctt
    11941 gctgcacgtc gaacgaaagg cgtgtacggt gggcgagcgc ggtgtcgcct atagcaatga
    12001 gaccttctcc tttcgcgagc tgtgggattt tttcgtcggc gttatcgatt tcttgaaacg
    12061 gtatatttat ccccaattct tttaagatga ctttaagctg cgccacggag ctgcgcgagg
    12121 cggcatcagt gtagataata ttgagacgtg ttagcgtttc gcgaaatgac aacgaggcgt
    12181 ctgcggtaaa aaaccggatc gatgcgacac ggtttttcgc ccccacgcaa acgcctttgt
    12241 ggtaagtata gcctttgccc aaccgaaaaa actcaatgga ggagatgagc gcggcatcga
    12301 ccttgccttc gcgcaaatac gcaaccagac gcgatggaat gtccggcaca agagtcacgg
    12361 cggaagagtt ttcgagcgca ccccatagcg gccacgcgtt caaaaaattt accacaccca
    12421 agcgcacacg gcgttgtcga tagacgaaac atcgagtaaa tgtttactgt atatggtgtc
    12481 attgtggaat gtatagagcc tgcgctcgtt catgacaaaa aaaaacttta aagcgaaaga
    12541 aaaaatgacc cgtgcggatt accaagcgct catcgacact atttatcatc acaatcgaaa
    12601 atattatcgc gacgacgcgc ctgaaatcag cgacgccgac tatgacgaat tgatgcgtct
    12661 tttacaggag ctcgaagcca agcacccaga ctggcagcag agcgattcgc ccacgctcaa
    12721 ggttggaggc gaagtgcgcg aagatttcgc cgcagtcaaa cacgatccgc cgatgatgag
    12781 cctcgacaac gcgatggatg taggtgaact caaagcgttc catgaacgcc tggtgaaagc
    12841 ggggctcaag aatcccacgt accatgtgga gcccaagttc gatggactgg caatcgaact
    12901 tctttatatc gatggaaaat tagccgtcgg ctcgacacgc ggcaacggag acgtggggga
    12961 agacatcacg cacaacatac gcaccgttgc aaatgtgccg ctgcgccttt tgtgcgagaa
    13021 tccccccgaa cttttaagcg tgcgcggcga agtgatcatc aaactcgccg atttcttgaa
    13081 gctcaacgag cggcaagagc gcgaaggaaa aaaacttttt gcgaacccgc gcaacaccgc
    13141 cgctggcgct ttgcgtcagc aggattccaa agaagcggcg ggtaaatcgc tttcgttctt
    13201 tccttattca ttgggtaaaa ttgtcgatgc aaaaaatcga ttcgcgaaag cgctacgctc
    13261 gcagcagaaa atttggcaag aggtgtttgt ggaattgggg tttcaaattg ccgcgtacca
    13321 caaaaaaaca aaccttgcag ggattgaagc gtattatcaa gaaatgcagg aggaacgtgc
    13381 atcgctgcct tatgacttgg atggccttgt cgtaaaactc gacgacacca gcgaatggaa
    13441 agattttggc gcgacgacca aagcgccgcg ttgggcgatt gctctcaaat ttccgccccg
    13501 cgtgggtttt acacgtttgc agaatgtggc gtaccaagtg ggacgcactg gcgtcgtcac
    13561 ccccgtggcg gagctcttgc cgattaactt aggtggcgtg ctggtttcac gcgcgtcgct
    13621 ccacaacgcc gatgaaatcg aacgcttgga tttacatgaa ggcgacagcg tcgaggtggt
    13681 gcgttcgggc gatgtgatcc ccaaagtggt gcgtgtattt cccgaaaagc gcgcacaaaa
    13741 tgcccgcccc atcgagtttg tcgccgcgtg ccctgcgtgc ggaagaaaat taatgagaga
    13801 agatgtgttc taccgctgtg tgaatcccac ttgcgaaggc atcaacgaag ccgcgttgca
    13861 attctttgtg tcgaagggcg gcattgacgt ggagtttctg gggcaagaat gggtggcgaa
    13921 actttacaaa gctggagtca tccaagattt tgccgacttt ttccgcttaa cgcaaatcga
    13981 ccttttaacg ttcgagggta tgggagaaat tttagcggca aaaattttga aatctatcgc
    14041 cgcaagacgc accattccct tggcaaaatt cttaaccgcc atcggtatcg ccaacgtggg
    14101 t
//
LOCUS       DAOMPD010000008        14051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1862_length_14051_cov_2.812161, whole genome shotgun sequence.
ACCESSION   DAOMPD010000008 DAOMPD010000000
VERSION     DAOMPD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 14051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14051
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1862_length_14051_cov_2.812161"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLY93_00640"
     CDS             <1..646
                     /locus_tag="PLY93_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRP68026.1"
                     /translation="LAVAYTLHPHPPFTLPKRDNYTAENSPRKEYYKALKYSDDTLKD
                     FFTAAAREMWFKDTVFVLMADHAYEETQGRDAFHIPLLVYAPGIVTPRVDNRIVSELD
                     VLPTLVDLLQISTFHASMGKSALAEGERVAYLDVEHAAGIVQHKDGFSQVALLGSDTL
                     VGAYDLVNDAQWKTKLKDNLYTKEEIQQMLDYVGVMGYAIRENKIAERTPKSLF"
     gene            949..2154
                     /gene="tuf"
                     /locus_tag="PLY93_00645"
     CDS             949..2154
                     /gene="tuf"
                     /locus_tag="PLY93_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRP68027.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITTVLAHKFGGKN
                     KAIAYAEIDNAPEERARGITIATSHQEYETEKRHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVSAVDGPMPQTREHILLAKQVGVPQIVVFLNKIDMLPEADRKEMQELVEMEVR
                     ELLTKYGFPGDDIPFVAGSGLKAVEGDTSDIGEGAILKLAETLDSYVKDPVRITDKPF
                     LMPVEDVFSITGRGTVCTGRVERGKVSVNEEIEIVGLRDTQKTIVTGIEMFRKLLDFG
                     QAGDNVGLLLRGTKKEDVERGQVIVKPGSVTPHKKFSGEVLVLTKDEGGRHTPFFDKY
                     RPQFYFRTTDVTGTVNLPQGVEMVNPGDNVTITAELISPIAMEEGLRFAIREGGRTIG
                     AGVVAKIIE"
     gene            2161..>2425
                     /gene="rpsJ"
                     /locus_tag="PLY93_00650"
     CDS             2161..>2425
                     /gene="rpsJ"
                     /locus_tag="PLY93_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRP68028.1"
                     /translation="MAAQKIRVKLKSFDQRLLDQSTGDIVGTVQRTRAKIAGPVPLPT
                     KVEKFTVLRSTFVYKDARDQYEMRTHSRLIDILEPTEDTIDALM"
     assembly_gap    2426..2435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2524..3150
                     /gene="rplC"
                     /locus_tag="PLY93_00655"
     CDS             2524..3150
                     /gene="rplC"
                     /locus_tag="PLY93_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRP68029.1"
                     /translation="MQKGLLGKKIGMLSIWTDEGEQLAVTAIQAGPCPVLQVKTNEKD
                     GYAAVQIGFGAKADKSTTKAQKGHQKTIKEKSGSFVDEIVELRDYSTDKVVGDFLTCD
                     LFAPGEKVFVSGTSKGKGFAGVVKRYKFSGGRMTHGSKFHRAPGSIGNRKIPGEVQKG
                     KRMPGHKGAKAATIKNIEIVKIIPEDNVVLLKGAVPGHKGSALFIYQN"
     gene            3158..3853
                     /gene="rplD"
                     /locus_tag="PLY93_00660"
     CDS             3158..3853
                     /gene="rplD"
                     /locus_tag="PLY93_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRP68030.1"
                     /translation="MATIKTYSNTGAANGEAQITDKLTSLKVNNALIWEIIKAEQANQ
                     RQGTHKTKTKAEVSGGGRKPWKQKGTGNARQGSTRSPQWRHGGIVFGPLPRDYRENIS
                     RQKKQVGLAHILAKKLQENKVVVYDGLAQEKVSTKGAFETISKITLGAKLQTEGRAKK
                     TLRANSNRARHNIAVVMASNETKDVKSLRNIPWVKPVNATRLLAASLFYNEGLVVSKA
                     ALAELDKKFSALL"
     gene            3862..4146
                     /gene="rplW"
                     /locus_tag="PLY93_00665"
     CDS             3862..4146
                     /gene="rplW"
                     /locus_tag="PLY93_00665"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRP68031.1"
                     /translation="MNVYDIIKKPLVSEKAEQLRAQNCYVFEVALASNKKMIKDAVRK
                     IFNVTPEKINTLILRSDAKANRHNIGYTKRRKKAYVFLKKSDKISLFEGV"
     gene            4150..4992
                     /gene="rplB"
                     /locus_tag="PLY93_00670"
     CDS             4150..4992
                     /gene="rplB"
                     /locus_tag="PLY93_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRP68032.1"
                     /translation="MPIKKFKPNTPTQRYKSVLKSSEVTESTPHKALLTFKKESAGRN
                     NQGRITSRRRGGGHKRRYRVIDFRRNKFNIPATVETVEYDPNRSANIALICYADGARS
                     YIIAPQGLKIGQKIISAPKVEIEVGNAAPLSSLPIGAHIHNIELTLGRGGQLARSAGT
                     FAIISGRDGDYTIIKLPSGETRKVHNRCIATLGVVGNHEHELVSLGKAGRSRWLGLRP
                     RVRGVAMNPVDHPLGGGEGKTSGGRHPVSPWGQPTRGYKTRNKRKTSERFIISRKVRR
                     RGGE"
     gene            4992..5267
                     /gene="rpsS"
                     /locus_tag="PLY93_00675"
     CDS             4992..5267
                     /gene="rpsS"
                     /locus_tag="PLY93_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRP68033.1"
                     /translation="MSRSLKKGPFIDQHLFKKVEKAKAANDKKPIKTWSRRSTIFPEM
                     VGMTFLVHNGNKFIPVYVNENVVGHKLGEFAPTRTYKGHTSLDKKKA"
     gene            5285..5623
                     /gene="rplV"
                     /locus_tag="PLY93_00680"
     CDS             5285..5623
                     /gene="rplV"
                     /locus_tag="PLY93_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRP68034.1"
                     /translation="MEAKSSVDQVRMSARKMRLVANEVRGEGYATAAGILKAMPLKGA
                     RIILKALNSAAANARVLNPNVDENALYVRKIFVDGGPLMKRFMPRARGRADRIQKKTS
                     KLTIVLSDED"
     gene            5652..6329
                     /gene="rpsC"
                     /locus_tag="PLY93_00685"
     CDS             5652..6329
                     /gene="rpsC"
                     /locus_tag="PLY93_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRP68035.1"
                     /translation="MGQKTNPIGLRLKITRTWDSVWYHKKETYAPTLHEDLRIRKHIK
                     ERFNKSGIVKVGIERFPEKIHVKLHSTKPGLVIGQQGKKIEALKAQLRTMVTKPLDVK
                     IIEVAKPDGSAQALAESVALQLEDRMAFRRAMKMALRSAIRAGALGVRVMVSGRLNGA
                     DMARREQYLEGRVPLHTLRAIIDYGTAEADTTFGKIGVKVWIYTGDYLEAKENEEDKY
                     TVKRREA"
     gene            6339..6746
                     /gene="rplP"
                     /locus_tag="PLY93_00690"
     CDS             6339..6746
                     /gene="rplP"
                     /locus_tag="PLY93_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRP68036.1"
                     /translation="MLAPKRLKWRKPHTLNLRGFSKGGNTVAFGDIGLKAVSGERITS
                     RQIEAARVAMMRHVRRGAKVWIRIFPDRPVTKKPAETRQGKGKGSVDHYVAIVKPGKV
                     MFEMGGADEKVMTEALTRAASKLPIKTKIIKRV"
     gene            6831..7079
                     /gene="rpmC"
                     /locus_tag="PLY93_00695"
     CDS             6831..7079
                     /gene="rpmC"
                     /locus_tag="PLY93_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRP68037.1"
                     /translation="MAKADKKKATKKIDFAELTDAELMRHHGDFRRELQEIRFGMVTA
                     SFTNNARVGSLKKDIARILTVLSQRQQKGKASKVAGKV"
     gene            7076..7357
                     /gene="rpsQ"
                     /locus_tag="PLY93_00700"
     CDS             7076..7357
                     /gene="rpsQ"
                     /locus_tag="PLY93_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRP68038.1"
                     /translation="MSEVKKSKKLIQGVVVSAKTPKTIVVSIAFRKQDTRFKKTIRLT
                     KNLMAHDEKGEAREGDTVIVEESKPFSKQKKFALAQIVTRKRDGAALVG"
     gene            7387..7779
                     /gene="rplN"
                     /locus_tag="PLY93_00705"
     CDS             7387..7779
                     /gene="rplN"
                     /locus_tag="PLY93_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRP68039.1"
                     /translation="MLQMLSWLKVADNTGARQVQVIKVLGGSKKRYAGVGDIIVVAVK
                     SATPAYGIKDSMGKKDHKKQVQKAVIVRTKKEVRRADGSYIRFDENACALLDKDNNPK
                     GTRIFGPVARELRDRQFMKIISLAPEVL"
     gene            7779..8123
                     /gene="rplX"
                     /locus_tag="PLY93_00710"
     CDS             7779..8123
                     /gene="rplX"
                     /locus_tag="PLY93_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRP68040.1"
                     /translation="MKPAPVKTKTRLKLKDEVVVIAGKNRKQRGEVLFLNLEKQQVVV
                     KGVNLRKRFARPTQENPKGGVIEIEMPIALSNVQYYDSKEKKPTRIAYGIDKNGKKTR
                     LSIASGRKKVLD"
     gene            8126..8692
                     /gene="rplE"
                     /locus_tag="PLY93_00715"
     CDS             8126..8692
                     /gene="rplE"
                     /locus_tag="PLY93_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRP68041.1"
                     /translation="MAAAKKDVARLRQLYNEKFRGELKEELAVKSIMQVPVLKKIVLN
                     VGAGFAVANPKSIDKVVTELGLITGQAPVKTRAKEAISNFKLREGLAIGVMVTLRGNR
                     MYEFLDRLVNISLPRVRDFNGLSPKSFDTRGNYSLGIKEQIIFPEINFDNVDNIHGMD
                     VTLVIQSEGPEHSLKLLEKFNFPIRKGK"
     gene            8695..8880
                     /locus_tag="PLY93_00720"
     CDS             8695..8880
                     /locus_tag="PLY93_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRP68042.1"
                     /translation="MAKKSMEIKAARKPKFSTRGYHRCPLCGRAHGYMRRFQMCRICF
                     RENASNGLIPGVSKSSW"
     gene            8888..9280
                     /gene="rpsH"
                     /locus_tag="PLY93_00725"
     CDS             8888..9280
                     /gene="rpsH"
                     /locus_tag="PLY93_00725"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRP68043.1"
                     /translation="MTNDLISDMLTRIRNAGRAGHETTEVRINNTCEKILDILKSEGF
                     IDGFVSFKSGASRMAKIEMRYYKNKAVFSAIERVSKPGRRIYLPWKEIRPTRNNLGLS
                     IFSTPKGVLSEKEAKFQHVGGEYLCRIY"
     gene            9289..9831
                     /gene="rplF"
                     /locus_tag="PLY93_00730"
     CDS             9289..9831
                     /gene="rplF"
                     /locus_tag="PLY93_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRP68044.1"
                     /translation="MSRIGNKIIQIPTGVSITASAESVTVKGPKGTLVSPLFQGLKLE
                     VNGSEAKLSRANDEKELRAKHGLVRSLIYNNVVGTSTGFTETLILQGVGFRAQKKGKD
                     IVMNLGFSHDVVFHEPTDVTIDVVENTKLKINGIDKQRVGQVAAQLRAFREPEPYKGK
                     GIRYETEHVRRKAGKTGSKK"
     gene            9840..10226
                     /gene="rplR"
                     /locus_tag="PLY93_00735"
     CDS             9840..10226
                     /gene="rplR"
                     /locus_tag="PLY93_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRP68045.1"
                     /translation="MASIARLNRQKSKRVARVRAHLRTANRAGKPRIAFHVSGRHLYA
                     QVFDDATGKTLVTCSTLEKSLQEAGVKSFTNKSFSAKLAEALVGKMQSKNLKLDTGYI
                     FDRGAKLYHGRVQSFAEKLREKGVKI"
     gene            10311..10886
                     /gene="rpsE"
                     /locus_tag="PLY93_00740"
     CDS             10311..10886
                     /gene="rpsE"
                     /locus_tag="PLY93_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRP68046.1"
                     /translation="MDIDGQTIELTERVVSMARVAKVVKGGRRFSFNALTVVGDGQNY
                     VGLGFGKAKEVPEAIRKSIEDAKKAVVKIPKKGTTVPHEVIGRFKSARVMLKPAAPGT
                     GIIAGASVRAVIELAGIHDILTKAVGSTNPLNIVKATLDGLRQMNSIEETKLRRGIPL
                     PVLFGAYAKERRDEKRKAMAAQAAVAAQPTT"
     gene            10897..11079
                     /gene="rpmD"
                     /locus_tag="PLY93_00745"
     CDS             10897..11079
                     /gene="rpmD"
                     /locus_tag="PLY93_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRP68047.1"
                     /translation="MKKVRVTQTRSEIRKSDKQKATLVALGLRGVGMSREHAVTPQLE
                     GMLKKVNFLVKVETIA"
     gene            11076..11582
                     /gene="rplO"
                     /locus_tag="PLY93_00750"
     CDS             11076..11582
                     /gene="rplO"
                     /locus_tag="PLY93_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRP68048.1"
                     /translation="MNQLKPNPGANKRIKRVGRGTSSGFGKTSGRGGKGQTARNGGNI
                     PARFEGGQWPLYRRIPKRGFQNPFRKEYSPVNVQSLAYLIVSGALTESAINIDALKKA
                     GFLPKSALAWKLLGKANGKESALSGKSIQVDAVSESAKKIVEAAGAKVELIEVKSKVL
                     IKGKKAAQ"
     gene            11648..11942
                     /locus_tag="PLY93_00755"
                     /pseudo
     CDS             11648..11942
                     /locus_tag="PLY93_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
     assembly_gap    11943..11953
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11954..12944
                     /gene="secY"
                     /locus_tag="PLY93_00760"
     CDS             <11954..12944
                     /gene="secY"
                     /locus_tag="PLY93_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRP68049.1"
                     /translation="QAYGITVYITSLHHEAMQGGKGMLISAGPGMSFTALTMLTITAG
                     TILLMWMGELMTERGVGNGTSLLIFAGIIAGAPAAVGQFLGDIKSNQADAILAIILLV
                     LFVTMIALSVLLNEGQRKIPLQYGKRIVGRRMVQGASQTLPIKVNAANVMPIIFAASL
                     MIFPSQIASYFGGRYQGFAEAIQKYLAPGAVTYFIIYIVLIIFFAYFYTSIQYNPKEI
                     AEALKKNGGYIPGVRPGAHTEETLERILNRITLSGAIFLAFIAIAPDIFIKLWSLEKY
                     ASLIHLFGGTSLLITVGVALDTLKQVESQLIMRHYDGFMDKNKKSTGRPRMVR"
     gene            12968..13531
                     /locus_tag="PLY93_00765"
     CDS             12968..13531
                     /locus_tag="PLY93_00765"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRP68050.1"
                     /translation="MQLIFLGPPGAGKGTQAKIFLERAGIVQISTGDILRAAVAKGTE
                     LGLQAKGFMDKGELVPDSIVIGIIEQRIQEDDCKKGFILDGFPRTIEQAKALDEILTK
                     QSIQLEHVINFNVPDEELVKRLLGRATAEGRSDDNPESIKNRLQVFKDKTQPLIRYYE
                     EKKILRHIDGVGSTTEIADRVKIVVGV"
     gene            13533..>14051
                     /gene="map"
                     /locus_tag="PLY93_00770"
     CDS             13533..>14051
                     /gene="map"
                     /locus_tag="PLY93_00770"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRP68051.1"
                     /translation="MGVNIYTNKEVTQIMESGRIAYETHMRIREKIKPGVTTAELDEV
                     AREFIYSKKAKPAFLGLYGFPASICTCVNDGVVHGIPNKKQVLQEGDIISVDLGVALN
                     GYFSDTAWTWPVGRVSEEAAKLVTVTQQCLFKGIAEAKPDNRIGAIGEVVQKHAEGNG
                     YGVVRQLVGHGIG"
BASE COUNT         4144 a         2970 c         3539 g         3377 t
ORIGIN      
        1 ccttgcggtc gcgtacactc tccatccgca cccgcctttc acgttgccca agagggataa
       61 ctataccgct gaaaattcac cgcgtaaaga gtactacaaa gcgctcaagt acagcgacga
      121 taccctcaaa gattttttta ccgccgcagc acgcgagatg tggttcaaag ataccgtctt
      181 tgtactcatg gccgatcacg cttatgaaga gacgcaagga agggacgcat tccacatccc
      241 actcttagtt tacgcgccgg ggatagttac cccccgcgtt gataaccgca ttgtttctga
      301 gttggatgtg ttgccgacgc tcgtcgattt actccaaata tcgacgttcc acgcgtcgat
      361 ggggaaatcg gcgttggcag aaggggagcg agtcgcctat ctcgatgtcg agcatgcggc
      421 aggcatcgtg caacacaaag acggattttc ccaggttgca cttttaggca gcgacacgtt
      481 ggtgggcgca tacgatttag tcaatgacgc gcagtggaaa actaagctca aggataatct
      541 ttatactaaa gaagaaatcc agcaaatgtt ggactacgtc ggtgtcatgg ggtatgccat
      601 tcgcgaaaat aaaattgcag aaagaacccc gaaatctcta ttttgattgt cagcctgacg
      661 caaatctcaa aactgcctat ctgtacttgg tggttcgggc aaagctcact ctttgccgag
      721 gacatggatt acaaaaccag tgcgagtttt aataaattga ggctttgccg tgtaaacggc
      781 ctcaatcaaa gttttgcgag gctgtagcgg tttcttaaaa ctcgatgagg cgcagtatag
      841 ggcagagcca aatgttttga gcgggcggca tcgatgaatt cgtgcctgcc gttaaacaaa
      901 gtgcctcgtg catcaaggtg cctcggcacc attaaaagga gaccggaaat ggcaaaagaa
      961 aaatttgaca gaagcaaacc ccacgtaaac gtcggtacaa tcggccacgt tgaccatggt
     1021 aaaaccactt tgacagcggc aatcacgacc gtgcttgcac acaagttcgg tggtaaaaac
     1081 aaagcgattg catacgcaga aattgacaac gcgcccgaag agcgtgcacg tggtatcacc
     1141 atcgcgacat cgcaccaaga gtacgaaact gaaaagcggc actacgcaca cgtcgactgc
     1201 ccaggccacg ccgactatgt aaaaaacatg atcaccggcg cagcgcagat ggatgcggca
     1261 attttagtcg tttcagcggt agacggtccc atgccacaaa cgcgtgaaca catccttctt
     1321 gcaaaacaag tgggtgttcc tcagattgtt gtgtttttaa ataaaattga catgctgccc
     1381 gaagcagatc gcaaagaaat gcaagagctc gttgagatgg aagtgcgtga attgctaacg
     1441 aagtacggtt ttccaggcga cgacattcct ttcgttgctg gttctggcct caaagcggtt
     1501 gagggcgata caagcgacat tggcgaaggc gcaatcctta aactcgctga aacgttagac
     1561 agctatgtaa aagatccggt tcgtatcact gataaaccat tcctcatgcc tgtggaagac
     1621 gtgttctcta tcaccggtcg tggtactgta tgtactggcc gtgttgagcg tggtaaggtt
     1681 tctgtgaacg aagaaattga aatcgttggt ttgcgcgata cgcaaaaaac catcgtcacc
     1741 ggtatcgaaa tgttccgtaa actcctcgac ttcggtcaag cgggtgacaa cgtgggcttg
     1801 ctgctccgtg gtacaaagaa agaagatgtc gagcgcggac aagtaattgt taagcctggt
     1861 tcggtaactc cacacaagaa gttttcaggt gaagttctcg tgctcacgaa agacgaaggt
     1921 ggtcgccaca ctccattttt tgacaagtat cgtccacagt tctatttcag aacaaccgac
     1981 gttacaggta ccgttaacct ccctcaaggt gttgagatgg tgaatcctgg tgacaacgtt
     2041 acaatcactg cagaactcat cagcccgatt gcaatggaag aagggctccg ttttgcgatc
     2101 cgcgaaggtg gtcgtaccat cggtgcgggt gttgtagcga aaattattga gtaagcaaac
     2161 atggccgctc agaagatacg tgttaaatta aagtcgttcg accagcggct gttagaccag
     2221 tcgacagggg atattgtggg gacagtgcag cgcacgcggg cgaaaatcgc gggtcctgta
     2281 ccgctaccaa caaaagtaga gaaatttaca gtcctccgtt cgacgtttgt ttacaaagat
     2341 gcgcgcgatc agtacgaaat gcgtacgcac tcgcgcctta tcgatattct tgaacctaca
     2401 gaggatacta ttgatgcttt aatgannnnn nnnnngcttc agttgcctgc tggagtgatg
     2461 gtttatatta aatcataatc ggatataggt ttcggttata taaagtgaag taaagggaca
     2521 acaatgcaaa agggattact tggtaaaaaa ataggtatgc tcagcatctg gacagacgag
     2581 ggcgagcaac ttgcggttac agcaattcag gctggtccct gtcctgttct tcaagtgaag
     2641 acgaatgaaa aagacggtta tgcagccgta cagattggct ttggcgcgaa agcggataag
     2701 tcgacgacga aagcgcaaaa gggacatcag aaaactatca aagaaaagtc aggttctttt
     2761 gtcgatgaaa ttgttgaatt gcgtgactat agcaccgata aagttgtggg agactttctc
     2821 acctgcgacc tttttgctcc tggtgagaag gtttttgtct ctggtacaag caaaggcaaa
     2881 ggctttgcgg gcgttgttaa gcgttataag tttagcggtg gtcgcatgac gcacggttca
     2941 aagttccatc gcgctccggg ctctattggt aaccgtaaaa ttcctggtga agtacagaaa
     3001 ggaaagcgta tgcccggcca taaaggggca aaagcagcca ctatcaaaaa catcgaaatt
     3061 gttaaaataa tccctgaaga caacgtcgtc ttattgaaag gtgcagtgcc cggtcataaa
     3121 ggcagcgctc tttttattta tcaaaattag gatttatatg gcaacaataa agacatactc
     3181 gaataccggc gcagcaaacg gcgaagcgca gattactgac aagttgactt cgcttaaagt
     3241 caataatgcc ctcatttggg agattatcaa agccgaacaa gcaaaccaac gccaaggtac
     3301 gcataaaact aaaactaagg ccgaggtctc cggtggtggc agaaagccgt ggaagcaaaa
     3361 aggcacaggg aatgcacgcc aaggttcaac gcgctcgcca caatggcgtc atggcggtat
     3421 tgtttttggt ccattaccgc gcgactaccg tgagaatatt tcacgtcaaa aaaagcaagt
     3481 cggtttggcg cacattttag caaagaagtt gcaagaaaac aaagttgtcg tgtacgacgg
     3541 tttagctcaa gaaaaagttt ccactaaagg cgcttttgaa accatcagca aaataaccct
     3601 gggcgcaaaa ttgcaaaccg aaggtcgcgc aaagaagacg ttgcgtgcca acagcaatcg
     3661 tgcacgccat aatatcgccg tggtgatggc atccaatgaa accaaggatg tgaagtcgct
     3721 gcggaatatc ccgtgggtta aaccagtgaa tgcaactcgt cttttagctg catcgctctt
     3781 ttataacgaa ggtttagtag tgagtaaggc ggcattggca gagttggata aaaagttttc
     3841 tgcgctgctt taagagggaa tatgaacgtt tatgatataa ttaaaaaacc attagtctct
     3901 gaaaaggcag agcaattacg cgcacagaat tgttatgttt ttgaagtggc cttggcttca
     3961 aacaaaaaga tgataaaaga cgccgttcgt aaaatattta acgtaacgcc tgaaaaaatc
     4021 aatacgctta tactgcgttc agacgctaaa gcgaatcgtc ataatattgg ctatacaaag
     4081 cgccgtaaga aggcatacgt cttcttgaaa aaaagcgaca aaattagcct ttttgaaggg
     4141 gtgtagtaaa tgccaattaa aaaatttaag cctaatacac cgacgcaacg ctataagtcg
     4201 gtactcaagt catccgaggt cacggaaagt actccgcaca aggcccttct tactttcaag
     4261 aaagaatcgg caggccgcaa caaccaaggc cgcattacaa gccgccgccg cggaggtggt
     4321 cacaaacgcc gctaccgcgt catcgatttc cgccggaaca aattcaatat tcccgcaacg
     4381 gttgaaacgg tcgagtacga tccaaaccgc tcggcgaata tcgcacttat ctgctacgcg
     4441 gatggcgcac gttcttacat tattgctcca caaggtctta aaatcgggca aaaaattatt
     4501 tctgccccca aggttgaaat tgaagtgggg aacgcagcgc ctttaagctc tctgcctatc
     4561 ggtgcgcaca tccacaacat tgagttaacg ttggggcggg gtgggcaatt ggctcgttca
     4621 gcgggaacat ttgctattat ttctggccgt gacggcgact acactattat caagttaccc
     4681 tcgggtgaga cgcggaaagt gcataatcgt tgcattgcaa cgcttggtgt cgtcggcaat
     4741 cacgagcacg aactggtctc tttgggaaaa gcgggacgtt ctcgctggtt aggtttacgc
     4801 ccccgcgtac gcggcgttgc gatgaaccct gtggatcacc cattaggtgg tggtgaaggt
     4861 aaaacatcgg gtggacgcca ccctgtgtcg ccatggggtc aaccaacccg tggttacaaa
     4921 acacgcaata aacgaaaaac atcagaaaga tttataattt cacgcaaagt gcgtcgtcgg
     4981 ggtggtgaat aatgtcacgt tcattaaaga aaggtccttt tatcgaccaa catcttttta
     5041 agaaagtcga aaaagcgaaa gcggcaaacg ataaaaagcc gattaagacg tggtcacgcc
     5101 gttccacaat ttttcctgaa atggtgggga tgacattcct agtacataat gggaacaagt
     5161 ttattcccgt gtacgtcaac gaaaacgtag tagggcacaa actgggcgag tttgcaccca
     5221 ctcgcactta taaagggcac acgagcctgg ataaaaagaa ggcgtagtta gttaaggaaa
     5281 taatatggaa gcgaaatcct cagtagatca agtacgcatg tcggcacgca agatgcgcct
     5341 cgtggcaaac gaagtgcgtg gcgaaggtta tgcgacggca gcgggcatcc tgaaagcgat
     5401 gccgcttaag ggagcgcgta ttatcttaaa agcgttaaac agcgccgccg ctaatgcaag
     5461 ggtgttgaat cctaatgttg atgaaaatgc gctttatgtg cgtaaaattt ttgtcgatgg
     5521 ggggcctttg atgaaacggt ttatgccgcg tgcacgaggc cgtgcagatc gtatccaaaa
     5581 aaagacatca aaattgacta ttgtgttgtc ggatgaggat taaaaagttt tgaacttaaa
     5641 ttaaggtata tatgggacaa aaaacaaatc cgattggctt acgcttaaaa ataacccgca
     5701 catgggatag tgtttggtat cataagaaag aaacgtatgc gccaacatta cacgaagact
     5761 tgcgtattcg taagcatatc aaagagcgct tcaacaaaag cggtattgta aaagtaggga
     5821 ttgaacgttt tcctgaaaag atccacgtta aattgcatag cactaagcct ggccttgtga
     5881 ttggccagca gggtaaaaaa attgaagcgt taaaagcgca gttgcgcacc atggtgacta
     5941 agcccctcga tgtaaaaatt atcgaggttg caaagcctga tggctcggcg caggcattgg
     6001 ctgagtcggt ggcgttacag ctcgaagacc gcatggcctt ccgtcgggcg atgaagatgg
     6061 cgctccgctc ggctatccgc gcgggtgcgt tgggtgtgcg cgtcatggtt tctggtcgtt
     6121 tgaacggcgc tgatatggcg cgccgcgaac aatacctgga aggccgtgtg ccgctgcata
     6181 cattgcgtgc tatcattgac tatggcactg ccgaggcaga taccaccttc ggtaaaattg
     6241 gagtcaaagt gtggatttat accggcgact atttagaagc aaaagaaaac gaagaagata
     6301 agtataccgt gaaacggcgg gaagcgtaag gataaaccat gcttgcacca aagagattaa
     6361 aatggcgtaa accacacacc cttaacttac gtggtttttc aaagggcggc aatactgttg
     6421 cgtttggaga tatcggctta aaggctgttt ctggcgagcg cattacttca agacaaatcg
     6481 aagctgctcg tgtggcgatg atgcggcatg ttcgccgtgg cgcgaaagtg tggattcgta
     6541 ttttccctga tcgcccagtg accaaaaaac cggctgaaac ccgccaaggt aaaggtaaag
     6601 gtagcgttga tcactatgta gcgattgtga aacccggtaa agtgatgttt gaaatgggtg
     6661 gcgcggatga aaaagtgatg actgaggcgc taacacgcgc agcgtcaaaa ctacctatca
     6721 aaacaaagat tatcaaaagg gtatagatat gcagcaaagt gacgctataa atccccgttc
     6781 tatcactctc attgacgggg tgtctaaaaa agagagaatg gatgtcttac atggctaaag
     6841 cagataaaaa gaaagcaaca aaaaagatcg actttgctga gttaaccgat gcagagttga
     6901 tgcgccacca tggtgacttc cgccgtgagt tgcaagaaat acgctttggc atggttacgg
     6961 catcgtttac aaataacgcg cgtgtgggtt cattaaaaaa agatattgca cgtatcttaa
     7021 ccgtgctctc gcaacgtcaa caaaagggta aagcctcaaa agtggccggt aaagtatgag
     7081 cgaagtaaag aaatcaaaga aattgattca aggtgttgtt gtttcggcaa agacacccaa
     7141 gaccattgtt gtgtcgatag cattccgtaa gcaagataca cgttttaaga aaacgatccg
     7201 cttgacgaag aaccttatgg cgcacgacga aaaaggcgaa gcacgcgaag gcgataccgt
     7261 cattgtggaa gagtcgaagc cattttcaaa gcaaaaaaag tttgcattag cgcaaatcgt
     7321 cacgcgtaag cgcgacggtg cggcgttggt aggctaacga taggaaacag gaaaacagga
     7381 taagatatgt tacagatgct ttcatggctc aaagtagccg acaatacagg ggcacgccaa
     7441 gttcaggtga ttaaagtctt gggtggttca aaaaagcgtt atgcaggtgt aggtgacatt
     7501 attgttgtcg ctgtaaaatc tgcgactccg gcgtatggca ttaaagattc gatgggtaaa
     7561 aaagaccata agaaacaagt acaaaaagcg gtgattgtgc gtactaaaaa agaagtgcgt
     7621 cgtgccgacg gctcgtatat ccgctttgac gaaaatgctt gtgcgttatt agataaagat
     7681 aacaacccaa agggcacccg tattttcgga ccggttgccc gggagttacg cgaccgtcaa
     7741 tttatgaaaa ttatttcttt agcaccggag gtgttgtaat gaaaccagcc cccgtgaaaa
     7801 caaaaacacg actgaaatta aaagatgaag tagtggtgat tgcaggtaaa aaccgcaagc
     7861 agcgcggcga agtgttgttt ttgaatttgg aaaagcagca agtcgtcgta aagggtgtca
     7921 atttacgcaa gcgttttgcg cgccccacgc aagaaaatcc caaaggcggg gttattgaaa
     7981 ttgaaatgcc gattgcgctt tccaatgtgc aatactacga ttcaaaagaa aagaaaccga
     8041 cgcggattgc gtatggtata gataaaaatg gaaaaaagac acgcctttca atagcgtcag
     8101 ggcgtaaaaa ggtgttggat taattatggc agcagcaaag aaagatgtgg cgcgtctccg
     8161 ccagctttac aacgagaaat ttcgtggcga gctcaaggaa gaactcgcag tgaaatcaat
     8221 tatgcaagtg cccgttctga aaaaaattgt cttaaacgtg ggcgccggtt ttgcggtggc
     8281 aaatcccaag tcaatcgaca aggttgtgac tgagttagga ctcatcactg gccaagctcc
     8341 ggtgaaaaca cgcgcgaagg aagcgatttc aaacttcaaa ttgcgcgaag gcttggcgat
     8401 tggcgtcatg gtgacattgc gcggcaaccg tatgtacgaa tttttagatc ggcttgtgaa
     8461 tatttcactc ccccgtgtgc gtgactttaa cgggttaagc ccaaagtcat tcgacacgcg
     8521 cggtaactac agtttaggga ttaaagaaca aatcattttc cccgaaatta actttgataa
     8581 tgtggataat atccacggaa tggatgtcac gttagttatt caatcggaag ggcctgagca
     8641 ttcgttaaag cttcttgaaa aattcaattt tccaattcgg aagggtaagt aattatggca
     8701 aaaaaatcga tggaaatcaa agcggcgcgt aagccgaagt tttcaacaag aggttatcac
     8761 cgttgtcctc tttgcgggcg tgcacacggc tatatgcgtc gttttcaaat gtgccgtatt
     8821 tgttttcgcg aaaacgcttc aaacggattg attccgggcg taagtaaatc ttcttggtga
     8881 gtataatatg acgaacgatt taatatcaga tatgttaacg cgcatccgca acgcgggtcg
     8941 tgcaggccat gaaaccacag aggtgcgtat caacaatacg tgcgaaaaga ttttagatat
     9001 tctaaaatca gaaggcttta tcgatggctt tgtcagcttc aagagtggag catcacgcat
     9061 ggcgaaaatt gaaatgcggt attataaaaa taaagcagtg ttttcggcga ttgagcgcgt
     9121 atcaaagcct ggccgccgga tctacttgcc ctggaaagaa atacgtccca ccagaaacaa
     9181 cttggggtta agtatttttt caacgcctaa aggcgtgcta tcagagaaag aagcaaagtt
     9241 tcaacatgtt ggcggagaat acctctgccg catctactaa gggaaaaaat gtcgcggatt
     9301 ggtaacaaaa ttattcaaat ccccacaggg gtttcaatta cagcaagcgc tgaaagtgta
     9361 actgtcaaag ggcctaaagg gactttggta tcgccacttt ttcaagggct taaattagag
     9421 gtgaacggca gcgaagctaa attatcgcgt gcgaacgacg aaaaagagtt gcgcgcaaaa
     9481 catggtttag tacgttcatt gatttataac aatgtcgtag gcacttcgac tggttttaca
     9541 gagacgctca tcctacaagg ggttggtttt cgtgcgcaaa agaagggcaa agacattgtc
     9601 atgaatcttg gtttttcgca tgatgttgtt tttcatgaac caaccgacgt gacaatcgat
     9661 gttgtcgaaa acaccaaact taaaattaac ggcattgata aacaacgcgt cggccaagtg
     9721 gctgcacaat tacgtgcatt tagagagccg gagccttata agggtaaggg tatccgttac
     9781 gaaaccgaac atgtccgccg caaagcgggt aaaacaggtt ctaagaaata agagggatta
     9841 tggcatcgat agcaagactt aatcgacaaa aatcaaagcg ggttgcgcgc gtgcgtgcac
     9901 acttacgcac tgcaaaccgc gcaggtaagc ctcgcattgc atttcatgtt tcaggccgtc
     9961 atctttatgc acaagtattt gacgatgcaa caggtaaaac tcttgttact tgttccacat
    10021 tagaaaaaag tttacaagaa gcgggagtga aatcgttcac caataaatct ttttctgcaa
    10081 aattggcaga agctcttgtc ggtaaaatgc agagcaaaaa tttgaaattg gatacaggat
    10141 atatttttga tcgcggtgca aagctgtacc acggtcgagt gcagagcttt gccgaaaagc
    10201 tccgcgaaaa gggagttaaa atttaattta tgagcgaaga agttaaacca cagggtaaaa
    10261 agcccggtcg taaaaaaaat gctcccaaag aaggcataaa aaagaacctt ctggatattg
    10321 acgggcagac cattgaatta accgagcgtg ttgtgagcat ggctcgcgtc gcgaaagtcg
    10381 ttaaaggtgg tcgtcggttt tcttttaatg cactaacagt ggtgggtgac ggccaaaatt
    10441 acgtcggttt aggctttggc aaagcaaagg aagtccccga agcgatccgt aaatctatcg
    10501 aagacgcaaa gaaagcggtg gtaaagattc ctaaaaaagg aacgacagtt ccgcacgaag
    10561 tgattggccg ctttaaatcg gcgcgcgtga tgcttaaacc agcggcgcct ggaactggta
    10621 ttattgccgg tgcatcggtt cgtgcggtga ttgaacttgc aggtatccac gatattttaa
    10681 caaaggcggt tggttcgaca aatccactga acattgttaa agcaaccttg gacggtctgc
    10741 gtcagatgaa tagtattgaa gaaacaaaat tacgtcgtgg cattccactt cctgttcttt
    10801 ttggtgctta tgcgaaagaa cgccgcgacg aaaaaagaaa agcgatggcg gcgcaggcgg
    10861 ccgtggctgc tcaaccaacg acgtgagggt tattgaatga aaaaagtacg tgtgacacaa
    10921 acgcgcagcg aaattcggaa aagcgacaag caaaaagcga cactcgtggc tttaggcttg
    10981 cgtggtgtgg gcatgtcgcg cgaacacgca gtgactccac aattagaagg aatgctgaag
    11041 aaagtaaatt tcttagtaaa ggtagagaca atagcatgaa tcaattaaaa ccaaacccag
    11101 gcgcaaataa gcggattaaa cgcgttggcc gcggtacatc ttccggcttt ggtaaaacat
    11161 cagggcgtgg cggtaaagga caaaccgcac gtaacggcgg caatatccct gcgcgctttg
    11221 aaggtggtca atggccgctc taccgccgta ttcctaaacg tggttttcaa aatccattcc
    11281 gcaaagaata ctcaccggtc aacgtgcaat ctttggcgta tttgattgta tcgggcgcgc
    11341 tgactgagag cgcaatcaat atcgatgcac tcaagaaagc gggctttttg cctaaaagtg
    11401 cgctggcttg gaaactttta ggtaaagcca acgggaaaga gtctgcactt tccggtaaaa
    11461 gcattcaagt agacgccgtc agcgaaagcg caaagaaaat tgtcgaagct gccggcgcaa
    11521 aagttgaact tattgaagtg aagtcgaaag ttttaataaa aggtaagaaa gccgctcaat
    11581 gatccgttcg atagtcacca ttttttccgt cgccgaattg cgtaaaaaaa ttctttttac
    11641 gttgggtatg ctttttcttt ttcgtattgg tgcacacatc acagtaccag gattagactt
    11701 taaggtgttg caagattatt ttgctaaagc ctcgtccagc ggcgccggta gtttgaccga
    11761 ctatatcgat ctttttgccg gtggagcttt taagcgtctt tcggtttttg cgttagggat
    11821 tatgccctat atctcggcgt cgattatcat gcaaatcatg atggtggcag tgccgtcgct
    11881 gcaacgttta gcaaaagaag gcgattacgg tcgtaaaaaa ataaaccagt atagtcgtta
    11941 tgnnnnnnnn nnnccaagcg tatggtatca ccgtttacat cacctcgctc caccacgaag
    12001 cgatgcaagg cggcaaaggc atgctaattt cagcaggccc agggatgtcg ttcactgcgc
    12061 tcacgatgct caccatcacc gcaggcacta ttttactcat gtggatgggt gagctcatga
    12121 ccgaacgcgg tgtgggtaac ggtacttcac ttttgatttt tgccggtatc atcgcgggtg
    12181 caccggctgc ggtaggccag ttcttgggtg atattaagtc gaaccaagca gacgccattt
    12241 tagcgattat tttactcgtc ctttttgtga cgatgatcgc cctttcggta ctcctcaacg
    12301 aaggccaacg aaaaatccct ctacaatacg gtaagcggat tgtaggccgc cggatggtcc
    12361 aaggagcaag tcaaacgcta ccgattaaag tgaacgctgc aaacgtgatg ccaattattt
    12421 ttgccgcatc gcttatgatt tttccctcac aaattgcgag ctattttggc ggacgttacc
    12481 aaggttttgc cgaggcgatt caaaaatatt tagcgccagg cgcagtcaca tattttatta
    12541 tctacattgt actcattatc ttctttgctt acttctacac ttcgattcaa tacaacccta
    12601 aagaaattgc agaagcgttg aagaaaaacg gagggtacat cccaggagta cgcccaggcg
    12661 cgcacacaga ggaaacactc gaacgcattt tgaatcgcat tactttatcg ggtgcaattt
    12721 ttctggcttt tatcgccatc gcaccggata tttttattaa actctggagc ttagaaaaat
    12781 acgcgtcgct catccatctc tttggtggca catcgctcct cattactgtg ggtgtggcgt
    12841 tagatacact gaaacaagtg gaaagccaac tcatcatgcg ccactacgat ggtttcatgg
    12901 acaagaataa gaaaagtact ggtcgacccc gaatggttcg ttaacctatc atgaatggag
    12961 gttacacgtg caattgatat ttttaggccc gccaggggca ggtaaaggaa cgcaggcaaa
    13021 gattttttta gagcgcgctg gtattgtaca aatttcaacc ggtgacattt tgcgtgcggc
    13081 tgtagcaaaa ggcacagagc ttggtcttca ggcaaagggc tttatggata aaggtgagtt
    13141 agtccccgat tccatagtta ttggtataat tgagcaacgg atacaagaag acgattgcaa
    13201 aaaaggcttt attctcgacg gttttccccg taccatcgaa caagccaaag cgttagatga
    13261 aattctaaca aaacagagca ttcagttaga gcacgttatt aattttaacg tgcccgacga
    13321 agagttagtg aagcgtcttt tggggcgcgc cactgccgaa ggccgttcgg acgacaaccc
    13381 cgaatcgata aaaaatcgct tgcaagtttt caaagataag acacaaccgc ttatccgtta
    13441 ttatgaagaa aagaaaatcc tgcgtcatat cgatggcgtg ggatcgacca ctgaaattgc
    13501 cgacagagtt aaaattgtag tgggagtgtg atttgggcgt taatatttat acaaataaag
    13561 aagtaacgca aatcatggaa tcggggcgta ttgcgtacga aacgcacatg cggatccgtg
    13621 aaaaaataaa acccggcgtg acaactgctg agctggacga ggttgcacgc gaatttattt
    13681 attctaaaaa agcaaaaccc gcttttttag gactttatgg ttttcctgca agcatctgca
    13741 cctgcgtcaa cgatggtgtc gtgcatggaa ttcccaataa aaagcaggtc ttgcaagaag
    13801 gcgatattat cagcgtcgac ttgggagtgg cgttgaatgg ttatttttca gacaccgcgt
    13861 ggacatggcc ggtgggtcgt gtcagcgaag aagcggcgaa attggtgact gtcactcagc
    13921 aatgcctctt caaaggtatt gccgaggcaa agccagacaa ccgcatcggt gcaattggcg
    13981 aagtggtgca aaagcacgcc gaaggtaatg gctatggtgt ggtgcgccag ttagtggggc
    14041 atggtattgg c
//
LOCUS       DAOMPD010000009        13992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_1872_length_13992_cov_3.515462, whole genome shotgun sequence.
ACCESSION   DAOMPD010000009 DAOMPD010000000
VERSION     DAOMPD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1872_length_13992_cov_3.515462"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLY93_00775"
     CDS             <1..1074
                     /locus_tag="PLY93_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009882 - blue light photoreceptor
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HRP68052.1"
                     /translation="MKRLTYISTFSNPLSPEEIETIAERSIANNTRDNLSGVLFCFNN
                     IFYQILEGPEDTLNRCYARILRDPRHKNIFCLEVESNIEKREFGDWAMKTVRLDESAD
                     SLIRPIRTMLNSMARTHYVLERYSPHEILRGLQTGENPLYWKIKTSDKVILFSDIFSS
                     STFAEALGGDQFEDLLQTYYGITNRSIEESGGTISKLTGDGFMAYFDGSQVEKALDAS
                     LEICRRLDDVRKSAPEGSPLRYLYSGIGLSAGLVREGNIGSKIKYDYTLLGDSVNTAA
                     RLESVTRKTDYLLVFDEKILAKIPKPSSLRPLGRYIPKGKTERINIYSIDYPYTKRNI
                     STDAIKKSILQLHNPQDNSPQLQ"
     gene            1090..2124
                     /locus_tag="PLY93_00780"
     CDS             1090..2124
                     /locus_tag="PLY93_00780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRP68053.1"
                     /translation="MKNTIDSIVSRGILPDAILRLAIRKLNRRRLKEISFDDIELAQA
                     KTQEVVQILRSSEIAVLTEKANEQHYEVPADFFNLVLGKHLKYSSCYFLTGKESLDEA
                     EFLMLNETIEMARIQDGDEILELGCGWGSLSLFMAQKFPRSKITAVSNSKSQKAFIDA
                     KAKQSNLKNLNVVTVDINNFNPKKKFDRVVSVEMFEHMRNYETLFSRIHSWLKTNGTL
                     FIHIFAHRDYPYFFENKDADDWMSRYFFSGGIMPSNHLFSYFSRQFSLKEHRVFKGTH
                     YHLTAEHWLRNLDTNRLQVLDIFSNVYGKNNARRWLHYWRVFFLAVSELFRTKSGQVW
                     CVNHYLYKKL"
     gene            2121..2912
                     /locus_tag="PLY93_00785"
     CDS             2121..2912
                     /locus_tag="PLY93_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRP68054.1"
                     /translation="MKLTAKRLSELTAVPVANIRKWRERYGVLTPERGDNGYLYYTSE
                     DYRLLLAIKNLLAKGMKISHIMRKGREILLEQIPTTHYRYEDMAFLEKVIDNRFSDLA
                     HELNATYRTNDLAYLINKVIHPQVVLVGQAWEAGLITVAAEHAYSRWLMGYLHQLTYK
                     YYPSTKQTSLFISFPGDEHELGAMMHFALMATEGQGGRFSGALPIDRLSEELGAGKYE
                     ELHVSVTIPKPRSEIKKFINRIYTLDKRIKIKIGGSGIESREELK"
     gene            2909..4150
                     /locus_tag="PLY93_00790"
     CDS             2909..4150
                     /locus_tag="PLY93_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68055.1"
                     /translation="MKNLAIVGSGIAGLGAAYLLRNDFNITILESGTFLGGHTNTIDI
                     PLSTGEKQSVDTGFIVFNEKTYPNLVKLFNELGVEYADSDMSFALYNTQTNLQWCSNG
                     LNGLFAQRKNIFSPQHYKFLLEANRFNRTLELDLKHGEVDGAFADYLHQRKFSDYFIA
                     NYIIPMSAAVWSTPPQKMLNFPAATFARFFVNHGFLSLYSGLQWKYVVGGSRVYVEKI
                     RSHFKGKVLLNTPVEKIEDLSEGVALSIPTGILHFDACLIATHADQALRMLAQPTVEQ
                     KRLLSPFQYEKNIAVLHSDTSVMQPLRRTWSSWNFKSGSDKSTVVYYMNKLQRISGDK
                     DYFVSLNDFQAIAPEKTYGIFHYEHPLFDSNTEKVQSELSLLNNSKRLFFSGSYFRYG
                     FHEDAYTSAILAAEAIKRQVL"
     gene            4150..4881
                     /locus_tag="PLY93_00795"
     CDS             4150..4881
                     /locus_tag="PLY93_00795"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRP68056.1"
                     /translation="MQEGIYEIDIKHTRTTPKTMKFRVRGYCFFIDVARFDSTDNFPG
                     YLHRFNRSDYSLLSDNSLSAELAAKKHLENSAKISADKVFLLANPRILGYVFNSVSLF
                     YYYKSGVHVATIVEVNNTFGEQKHYTLIGESQRSATKNFYVSPFISSFDQFNMHIEAP
                     NEKLTVKITTSNYKEVLLVASMTGLRHKLTPKNIIYFLFKYPWHTARIIILIHWYALR
                     LFFARVPFYGKKVSEAALLYKKVRR"
     gene            4911..6158
                     /locus_tag="PLY93_00800"
     CDS             4911..6158
                     /locus_tag="PLY93_00800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRP68057.1"
                     /translation="MGLSKISYGQLKVVELFPNGENYVLKGTQPGYTAELHVVNPEFY
                     RNTILYGEVGFGEAFVADNWRSPNLNVTLRWFAQNAQHLPGFSGSSVSKYAMNSLGFI
                     YRIKHFIRPNTLRVSRKNISEHYDLGNSFYELMLGKTMAYSSGIYYNANNSLDDAQEN
                     KFEVLCKKLQLNSEDNLLEIGSGWGGFAIYAAKKYKCRITTVTISKQQFDYVSAKIIA
                     EKLESLIEVRFADYRTLKGSYSKIVSIEMLEAVGIENLDTFFGVCNRLLQKDGLIALQ
                     YITYPEAKYEQYLKNNDFIQIHIFPGSCLLSNLEVLKSVHRTGDLMLVDLESIGQHYA
                     TTLRQWRLNIEKEESQILKMGYSDEFLRKWIYYLTYCEAGFAQRAISDVQIVFSRANS
                     TKSADFHEPKLIYRDNLQHTIEG"
     gene            6265..6897
                     /locus_tag="PLY93_00805"
     CDS             6265..6897
                     /locus_tag="PLY93_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68058.1"
                     /translation="MRYTGNAYDLNTGNFLYSENHSEFYENNRHIYSRVSYQYKDGKE
                     FASKFIRFASSKTQPTYELRDLRDGYVEGIKRNKQGTLYYLQKNFKEALNEKKITTPQ
                     PAVFDAGFDYFVRENFDAICSGINKSFYFAVPSKLDYYKFRLVRQNEKPLCRVNLEVQ
                     NILLRQLVKPIKLWYDMKTKRLLKYEGISNINGPDSKSLKVRIIFSYPKK"
     gene            6897..8216
                     /locus_tag="PLY93_00810"
     CDS             6897..8216
                     /locus_tag="PLY93_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP68059.1"
                     /translation="MSWSKISLYALAESGAFAVEFFVRLQLLIFYTDTLGIDSSLASL
                     AAGIGLFWDAFIDPFVGSYSDTFKSRWGNRKPFLIAGGILIIPSLYWIFSPSLSENTL
                     LIFIQIAAANLILNTGITLVTVPHAAISAELSRDAKVRIRIFAARLIFANIGLILGLL
                     VPAAILKWGREVGYTDSLIYPRIALVIALIAMATIVICVLSLGKSTTPNVHGHRSFIK
                     DIKIALKNRLFIGLFVSGFIAYIAVAINSALARYYYEHFLHLREDEFVLVLIVFILVW
                     SSSVVFWVKIADYFGKDRPATLGVIGLGLMSIVVYPLLPTGQLVGPILAAIVGGVFTG
                     CIILFDSYVADVADLDRNKSGESREGLYFGVWKFGIKASRGIALVVAGFLLTAIDYHA
                     GESPNQVVSFRIALLFGPGVGSVFVIAAIVFYFFVGKTIRTSNTIHS"
     gene            complement(8211..9083)
                     /locus_tag="PLY93_00815"
     CDS             complement(8211..9083)
                     /locus_tag="PLY93_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRP68060.1"
                     /translation="MDNEERKAIFIILFSTILGAALAFVAADATSSYSPIYTAVIAAY
                     GIQWLAFIPAFILKTERFYDLVGSLTYTSIVLYAVVVRPHLDIRSSVIAIFIIVWALR
                     LGSYLFLRVRRDRGDKRFANVRQSFWRFLMAWTLQGLWVTFSLCAALAALLSEREILP
                     DIFFWNGAFLWFAGFSIEIIADNQKRKFRKEKNNIDKFITTGMWAWSRHPNYFGEIVL
                     WIGIAVLAFPVLEGWQLLTLISPVFIFLLLTKGSGIPLLEAAADKRWGDQTEYEKYKK
                     STSILIPLPPRKKL"
     gene            9306..10577
                     /gene="hisS"
                     /locus_tag="PLY93_00820"
     CDS             9306..10577
                     /gene="hisS"
                     /locus_tag="PLY93_00820"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRP68061.1"
                     /translation="MTQLSTQPYKGTRDFFPDAMRFREKMFAVMHKTVRSYGFDQIDA
                     PILEPIDIYLAKTSEEIVGQQIYSFTDRGERKIAIRPEMTPTVARMVAARFHETPKPI
                     RWYSIPNVWRYEQPGKGRLREHWQLNCDIFGAPNEKLADLEILNLAIDLMRGYGANES
                     HFKLYLSHRGILNGVLGKELKLPPEKWAGVARIMDKRAKIKPDAYIEMLRAEGVGDNE
                     QQELDLFLNEKITYLKKKSHLEGASYVVEMLQSLEEIGLNAFVEYDPSVVRGFDYCTG
                     LVFEVFDAHPDNRRSLFGGGRYNNLVGAFGKENLNAVGFGMGDVTLEDFLRTHQLLGD
                     VPRKVELYIALMDEKLLEHAMQLASTLRARNLSVEVSLGAGKLGKQFQEAEKKGIPRV
                     LILGEDEAKSGVYSVKTLTTGEQVKLKATEI"
     gene            10704..11063
                     /locus_tag="PLY93_00825"
     CDS             10704..11063
                     /locus_tag="PLY93_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRP68062.1"
                     /translation="MSVKKYTGSCHCGEVRYEVQLDLTQPVTACNCSMCGRSGTYLTF
                     VSENDFVLKSGSDSLQDYQFNKKHIHHLFCKVCGIKPFARGAAPDGSSTIAVNVRCLD
                     GIEFDKLQVQHYNGKSL"
     gene            11161..12018
                     /locus_tag="PLY93_00830"
     CDS             11161..12018
                     /locus_tag="PLY93_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68063.1"
                     /translation="MAKTNTRANQRTTNQDRNTKTTLSLADKLFAITTRNYENSAEKF
                     LPKKHKNKLAIHDFQRTHKETLTKLEPITTASLPIKETEGKLLTLGRTVARRTMVVMA
                     RAALFIIAISAGLTLISYIADDSIRIEDERGKNALNNEIRAKEKAVSLNVMRKRKKIG
                     EFTVAKGSRLRIVKATNGDTFRPEVAFTGESADFKLKYKGKTALIVTNGPFHAKVKIA
                     QGANNDVHLRFKETGTLLPVGEPEFSIEVVKGDVQIAETDDNVYEEYKTGEQAVFTLS
                     SSDSGSENL"
     gene            complement(12030..12596)
                     /locus_tag="PLY93_00835"
     CDS             complement(12030..12596)
                     /locus_tag="PLY93_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP68064.1"
                     /translation="MTGKSFHERPAYSRQQREADFQRIYNKSKNRVLNYISRTVRDYD
                     EALDLLQEVFITFYDKLPDLDISTDRIESWLVKTARNVILTYVRDTHRRLTRDLQNTQ
                     ESDTWEKDIQVGKVYQKELEKHIDDFLDSLNDSERNLFILQKIEQVKYNDLEKILNIP
                     QRTMKRMAANLLQRLKERTTFDKDYLEN"
     gene            12664..13854
                     /locus_tag="PLY93_00840"
     CDS             12664..13854
                     /locus_tag="PLY93_00840"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRP68065.1"
                     /translation="MDPQGQWQNKFQALIDAFVNNFSQKREHGAALCVFQNGEKVVDV
                     WAGMADLKTRKMWQQDTLVTIFSVTKALTALCFLILALRKKFDYDKPVAYYWPEFALR
                     GKNNISCRLLLEHRASLYAIDTPLMLYDYVENPTKVYNTLIHQQPLAVPGSLQGYGAQ
                     AWGMYAGALFQKITGESVGVFFAREVAAPLKVDAFIGLPHGLQNKMATIYPVTAIERL
                     VGVIPDLLCGDTTEGRIARAFISGNTSIKQAYMNPSTGDKGIDVLNDPILREIELPWV
                     NGVSNARSLAIIMAVFAQGGKAYGVTFADAPLMQTLIRENKLRDDLVLQKRLGWNLGF
                     LKEETHLFSPNTETFGHSGMGGSLAFADPKVRVSFAYTCNKMDAKIRPDKTLALCHAL
                     YKCV"
BASE COUNT         4191 a         2968 c         2935 g         3898 t
ORIGIN      
        1 atgaaaagat taacctacat cagcactttt tctaatccgc tatcgcctga agagatcgaa
       61 acgatcgccg aacgatcgat tgcgaataac acccgtgaca acctttccgg cgtactcttc
      121 tgctttaata acatatttta tcaaatcctg gaaggccctg aagacaccct caaccgttgc
      181 tatgcccgta ttctgagaga cccccgacac aaaaacattt tttgcctgga ggtagaaagc
      241 aacatagaaa aacgagaatt tggcgactgg gctatgaaaa ctgtgcggct cgacgaatca
      301 gcagacagct taattcgccc cattcgaacc atgttaaatt caatggccag aacgcattat
      361 gtgcttgaaa gatattcgcc gcatgaaatc ttgcgggggt tacaaactgg agaaaaccct
      421 ctttattgga aaattaaaac cagcgacaaa gtaatattgt tttcagatat attttcttcc
      481 tccacttttg cagaagcgtt agggggcgat caatttgaag atttgttaca aacgtattat
      541 ggcatcacta atcgctctat cgaagaaagc ggaggcacta tttcaaaatt aacgggcgac
      601 gggttcatgg cttactttga cggttcgcaa gtagaaaagg cactcgacgc atcgcttgaa
      661 atttgccgcc gtttagacga tgtgcgtaaa agtgcgccag aaggtagccc tttacgctat
      721 ctttactcag gtataggcct ttcagcgggt ttagttagag agggcaatat cggttcaaaa
      781 ataaagtacg attacactct actgggcgat tctgtcaaca cagcggcgcg tctcgaaagc
      841 gtgacgcgaa aaactgatta tcttttggtc ttcgacgaga aaatactcgc taaaattcca
      901 aaaccttcgt cattacgtcc gttaggtcgt tatataccta aaggcaaaac cgaacgtata
      961 aacatttatt ctatagacta cccttacacg aagcgtaaca tcagtacaga tgcaataaaa
     1021 aagagcattt tacaattaca taacccgcaa gacaattcac cacaattaca ataattcagg
     1081 acacctttta tgaaaaacac aatcgactca atagtatctc gcggcattct acccgatgcg
     1141 attcttcgtt tagctatacg caagttaaat aggcgccgtt taaaggaaat tagttttgac
     1201 gatatcgagc ttgcgcaggc taaaacacag gaagtcgttc aaattttgcg ctcatcagaa
     1261 attgccgtct tgacagagaa ggcaaatgag cagcattacg aagtgcccgc tgactttttt
     1321 aacttagttt taggaaaaca tcttaagtat agtagttgtt attttctaac cggaaaagaa
     1381 agtttagacg aggcagaatt cttgatgctg aacgagacaa tcgaaatggc gcgcattcaa
     1441 gacggcgatg agattttaga attaggctgt ggttggggtt cgttgagtct ttttatggct
     1501 caaaaatttc cacgtagtaa aattacggca gtatcgaact cgaaaagtca aaaagctttc
     1561 atcgacgcca aggcaaagca aagcaatttg aagaacttaa atgtcgtcac tgtcgatatc
     1621 aacaacttta acccaaagaa aaaatttgat cgtgtcgttt ctgtggaaat gttcgagcac
     1681 atgcgcaatt acgaaactct tttttctcgt attcactcat ggctcaaaac aaatggtact
     1741 ttatttatac acatttttgc ccacagagat tacccttatt tttttgaaaa taaggacgct
     1801 gacgattgga tgagtcgata tttcttttcg ggaggcatca tgccctctaa tcatctgttt
     1861 tcttattttt ctcgtcaatt ttcacttaaa gagcatagag tattcaaagg tacgcactat
     1921 catctgaccg ctgagcattg gcttcgtaat ttagacacga atcgtttgca agtattagat
     1981 attttttcca atgtttatgg aaaaaataac gctcgacgtt ggttgcacta ttggcgtgta
     2041 ttttttttgg cggtgtcaga gctttttcgt acaaaatcgg ggcaggtttg gtgcgttaac
     2101 cattatcttt acaaaaaatt atgaagctta ccgcaaagcg tctaagtgaa ttgaccgcag
     2161 ttcctgtggc aaatataaga aaatggcgcg aaagatacgg tgttctaaca cccgaacgtg
     2221 gggataacgg ctatctctac tatacgtcag aagactaccg tttattgtta gcgataaaaa
     2281 acttgctcgc aaaggggatg aaaatttctc atataatgcg caaaggacgc gagatactac
     2341 tcgaacaaat tccgacgacc cattatagat acgaagatat ggctttttta gaaaaagtca
     2401 ttgataatcg cttctcagac ttggctcatg aactcaacgc aacttatcgc acaaatgatt
     2461 tggcctatct tatcaataaa gtcattcatc cacaagttgt tcttgtgggc caagcttggg
     2521 aagctggttt gataaccgtc gcagccgaac acgcatattc gcgatggcta atgggatacc
     2581 tccatcagtt aacgtacaaa tattacccca gtacgaagca gaccagtctt ttcataagtt
     2641 ttcccggcga cgaacacgaa ttaggagcaa tgatgcattt tgctttgatg gcaacagaag
     2701 gtcaaggtgg acgttttagc ggagcattac caatagatcg attatctgaa gaattaggag
     2761 cgggtaaata cgaagaatta catgtttcgg tcactatacc aaaaccgcgt agtgaaatca
     2821 aaaaatttat aaatcgaatc tataccttag ataaaagaat taaaataaaa attggtggtt
     2881 ctggaattga atcacgagag gaattaaaat gaaaaattta gccattgttg gtagcggaat
     2941 cgcaggctta ggcgccgcat atttgttacg caatgatttc aatataacaa tcttagagag
     3001 cggaacgttt ttaggagggc acaccaatac gatagacata ccgctttcaa cgggtgaaaa
     3061 acaaagcgtc gacacgggtt ttattgtttt caatgaaaaa acgtacccga atctcgtaaa
     3121 attattcaac gagctgggcg tcgaatacgc agatagcgac atgtcgtttg cactctacaa
     3181 tacacaaaca aatcttcagt ggtgctccaa cgggttaaac ggacttttcg cgcaaagaaa
     3241 aaatatattt tcgccgcagc actacaagtt tttattagag gccaatcgtt tcaatcgtac
     3301 tttagaatta gacctaaaac acggtgaagt ggacggcgct tttgccgatt acctacatca
     3361 aagaaaattt agcgattatt ttatcgccaa ttatataatt ccgatgtcgg ctgccgtatg
     3421 gtcgacccca ccgcaaaaaa tgctcaattt cccggcagct acctttgccc gcttctttgt
     3481 gaatcatggt ttcttaagct tatattccgg cttgcaatgg aaatacgtcg tgggcggtag
     3541 tcgggtttat gtcgaaaaga tacgttctca tttcaaaggc aaagttctcc ttaatacgcc
     3601 ggtagaaaaa attgaagatc tctctgaagg cgtcgcactc tctatcccaa cgggaatatt
     3661 acattttgac gcttgcctta tcgctacgca tgcagaccaa gctctccgca tgttggcgca
     3721 acctacagtc gaacaaaagc gacttttgtc gcctttccaa tatgaaaaaa acatagcagt
     3781 cttgcatagc gacacctctg ttatgcaacc gttacggcgg acttggtctt catggaattt
     3841 taaatccggt tctgataagt cgacagttgt ctattacatg aataaattgc agcgcatttc
     3901 aggcgataaa gattatttcg tatcgctcaa tgattttcaa gccattgcac ccgaaaaaac
     3961 ttacggtatc tttcattacg aacatccttt gttcgactca aatactgaaa aggtacaatc
     4021 cgagctttcg ctactcaata attcaaagag actctttttt agcggtagct attttcgtta
     4081 tggatttcat gaagacgctt acacatcggc aattttggcg gcagaagcaa ttaaaaggca
     4141 ggttctctaa tgcaagaagg tatttacgaa atagatatta aacatacgcg cacgactccg
     4201 aaaacgatga aatttagagt ccgtggttac tgttttttta tcgacgtagc tcgattcgat
     4261 tctaccgaca attttcccgg gtatttgcat cgctttaatc gttccgatta ctcgttgtta
     4321 tcggataatt ctttaagcgc agaattagcg gcaaagaaac atcttgaaaa ttcggctaaa
     4381 atttcagcgg ataaagtatt tcttttagct aacccgcgta ttctgggtta tgtattcaat
     4441 tctgttagct tattttacta ctataaatca ggggtacacg tggctactat tgtagaagtg
     4501 aataacacgt ttggcgaaca aaagcattat accctcatcg gagaatcgca gcgttcagca
     4561 acaaaaaact tctacgtttc gcctttcatt tcttctttcg atcaattcaa tatgcatata
     4621 gaagccccca atgaaaaatt aacggtaaaa attacaacat ccaattataa agaagttttg
     4681 ctggttgcct cgatgacggg tttacgccat aaattgactc caaagaatat tatttacttt
     4741 cttttcaaat acccatggca tacggcgcgt ataataattc tcatccactg gtatgcgctg
     4801 cgcctcttct ttgctagagt ccctttttac ggtaaaaaag tgtctgaggc ggcgctactt
     4861 tataaaaaag tacggagata atataaatga ttttcaaaaa aatatttcaa ttgggtttat
     4921 ctaaaatctc ttacggccaa ctaaaagttg tcgaactatt tccaaacggc gagaattatg
     4981 tacttaaagg tactcaaccg gggtacacgg ctgaactaca tgtggtcaac cctgaatttt
     5041 atcgcaacac gatactctac ggtgaagtgg gttttggaga agcttttgtc gctgataatt
     5101 ggcgatctcc aaatctcaat gtgactctac gctggtttgc tcaaaacgca caacacttac
     5161 ccggtttctc gggatcgagt gtctccaaat acgctatgaa ttctctgggg tttatctatc
     5221 gtataaaaca ttttatcaga cccaatacgc tgcgtgttag tcgtaaaaat atttcagaac
     5281 attatgattt agggaactct ttttacgaac tcatgttagg taaaacgatg gcgtattctt
     5341 ctggaatata ctacaatgct aacaattcgc tcgacgatgc tcaagaaaat aaatttgaag
     5401 tcctttgtaa aaaactacag ttgaactcag aggataattt actcgaaatc ggtagcggat
     5461 ggggtggctt tgctatttat gcagccaaaa agtacaaatg ccgcatcact acagtgacaa
     5521 tatccaagca gcaatttgat tacgtaagcg caaaaattat cgcagaaaaa cttgaatctt
     5581 tgatcgaggt gcgttttgcc gattatcgta ctttaaaggg tagctatagc aaaatagtat
     5641 caatagaaat gctcgaagcc gttggcatag aaaatttaga cacttttttc ggtgtgtgta
     5701 atagactatt acaaaaagac ggattgatag cgttacagta catcacttat cctgaagcga
     5761 aatacgaaca gtatcttaaa aataacgatt ttatacagat tcatattttt cccggttcgt
     5821 gtttactgtc aaatcttgaa gttttaaaat cggtacatcg cacaggcgat ctcatgcttg
     5881 tcgatcttga aagtattggg caacactatg cgacaacatt gagacaatgg agattaaaca
     5941 tcgaaaaaga agaatcgcaa attctaaaaa tggggtatag cgatgagttt cttcgaaaat
     6001 ggatttatta cttaacgtat tgcgaagcgg gctttgccca gagagcgatt agcgacgtac
     6061 agattgtttt ttcccgtgca aactcgacaa aaagcgcaga cttccatgaa ccaaaattaa
     6121 tttataggga caatttacaa catacgattg aaggataacc ggatgcgact tacactattt
     6181 tcttggatat ttttacaagt taagaaaatt ttagctcttg caattttatt tgtatttgca
     6241 agtcaaatag aggcgaaaaa tgtcgtgcgt tatacaggca acgcatacga tttaaacact
     6301 ggaaatttct tatattctga aaatcatagc gagttttatg aaaacaatcg acatatatac
     6361 tcgcgcgtct cataccaata taaagacggc aaagaatttg cgtcaaaatt cattcgcttt
     6421 gcatccagta aaactcaacc tacgtacgag ctgcgtgatt tgcgtgacgg gtacgtagaa
     6481 ggcataaagc gtaacaaaca aggcactctc tattatttac aaaaaaactt caaagaagcc
     6541 ttaaatgaaa aaaaaataac aacgcctcaa cctgctgttt ttgacgcagg tttcgattat
     6601 tttgtaagag agaattttga cgctatttgt tcgggtataa acaaatcgtt ttactttgcc
     6661 gttccgagta aactcgatta ttacaaattt cgattagttc gtcaaaatga aaaaccttta
     6721 tgtcgagtaa atcttgaggt tcaaaatata ctgcttaggc aactcgtaaa acctataaag
     6781 ctttggtacg atatgaaaac aaaaagacta ttaaaatacg aaggcatctc taatatcaat
     6841 ggacccgaca gcaaaagtct aaaagtccgc atcattttta gctatcccaa aaaataatga
     6901 gctggtcaaa aatatcgctc tacgcgctgg ccgaatcggg cgcctttgcc gttgaatttt
     6961 tcgtccgctt acaacttctt atattttaca cagatacact gggtatcgat agttcattag
     7021 cctctctagc tgcagggatc ggcttatttt gggatgcctt tatcgatccg tttgtcggtt
     7081 cttattcaga cacttttaaa agccgctggg gaaatcgtaa accatttctt attgccggtg
     7141 gtattcttat aataccgtct ctatattgga ttttctcacc cagtttgagt gaaaacactt
     7201 tacttatttt tattcaaata gcggcagcaa atttaatatt aaatacggga ataacattag
     7261 ttaccgtacc acacgcagct atatctgccg aactctcgcg tgacgcaaaa gtgcgtattc
     7321 ggatttttgc ggcacgatta atcttcgcta atattggttt aatacttggc cttctagtgc
     7381 ctgctgcgat tcttaaatgg ggtagagaag taggctatac ggattctctg atataccccc
     7441 gtattgcttt ggtgatagcg cttattgcaa tggcgacgat tgttatctgt gttctatctc
     7501 tcggtaaatc gacaactccc aatgtgcacg gccaccgttc atttataaaa gacataaaaa
     7561 ttgctttaaa aaatcgatta tttatcgggc tattcgtttc tggttttatc gcctatattg
     7621 cggttgctat aaattctgct ttagcccgtt attattatga gcatttttta catttgcgcg
     7681 aagacgaatt tgttttggta ttaatcgttt ttattctcgt gtggtcgtcg tcggttgttt
     7741 tttgggtaaa aattgccgat tacttcggaa aagataggcc ggccactctt ggcgtcatag
     7801 gtttaggtct tatgagcatt gttgtctatc cactgctgcc aacgggacag ttagttggtc
     7861 ccattctggc ggcaatcgtc ggtggagtgt ttaccggttg cattatctta ttcgattcgt
     7921 acgttgccga cgtggccgac ctcgatagaa acaaaagtgg tgaaagccgt gaaggcttat
     7981 acttcggagt ctggaagttt ggtatcaaag cttcgcgggg gattgcgctg gttgtcgctg
     8041 gttttttgtt gacggctatc gactatcatg ccggcgaaag cccaaaccaa gtcgttagtt
     8101 tccgaattgc gttgcttttt gggccaggcg tcggtagtgt ttttgttatc gccgcgattg
     8161 tgttttactt ttttgtcggc aaaacaatcc gtacatcaaa cacaatacac tcataatttt
     8221 ttccgaggtg gcaaaggaat taatatcgaa gtcgatttct tgtatttttc atactctgtt
     8281 tgatcgcccc atcgtttatc ggcagctgct tcaagtaaag gaatgccgct gcctttcgtc
     8341 aataataaaa aaataaatac cggcgagatt aatgtcaata gctgccatcc ttcaagaaca
     8401 ggaaaagcca agactgcaat acctatccag agtacaattt ccccaaaata attcggatgc
     8461 cgcgaccacg cccacatgcc ggtagtaata aatttatcta tattattttt ttcttttcta
     8521 aattttcttt tttgattatc tgcaataatt tcaatactaa atccagcgaa ccataagaac
     8581 gctccattcc aaaaaaatat atccggtaga atctcgcgtt ccgataagag ggcggctaag
     8641 gcggcacaaa gagaaaaggt aacccaaagg ccttgtagtg tccacgccat taagaatcgc
     8701 cagaacgatt ggcgcacgtt tgcgaagcgc ttgtctcctc tgtcgcgacg aacacgcaag
     8761 aaaagataac tcccaagcct taatgcccaa acgataataa atattgcaat gacactcgac
     8821 cgaatatcta ggtgtggacg tacaacaaca gcgtacaaaa caatcgaggt ataagttaga
     8881 ctaccgacaa gatcgtaaaa tctctctgtt ttgagtataa aagcaggaat aaatgcaagc
     8941 cactgtatac catacgccgc aattactgca gtgtagatgg gtgaatacga ggaagtcgca
     9001 tcggctgcaa cgaatgctaa agccgcacct aaaatcgtag aaaatagaat aataaatatt
     9061 gcttttcttt cctcattgtc catagactat tttgatattc attaaagcgc tgtcaatact
     9121 gattgggtcc tcaactcgcg cagcgtgtgc aaaaaaattg tctcaacgag ttggcgcaca
     9181 ggctctatgc caagaagtca taattattga cacagcgttt atggacaaca ttgcgtacgt
     9241 atccaaaatg ttactctcgc gagagtgaga ctgcaaaaaa ataactatac atcgaaatta
     9301 aatccatgac acaactttcc acccaaccct ataaaggcac gcgcgatttt tttcccgatg
     9361 cgatgcgctt ccgcgaaaaa atgtttgcgg tgatgcacaa gactgtgcgc agttatggtt
     9421 tcgatcaaat cgacgcacct atcctcgaac cgattgatat ttatcttgca aaaaccagcg
     9481 aagagattgt ggggcagcaa atttattctt tcacagacag aggtgagcgc aaaattgcca
     9541 tccgccccga gatgacgccg acggtggcgc gcatggttgc cgcgcgcttc catgaaaccc
     9601 ccaagcccat ccgttggtat tcgatcccca acgtgtggcg ttacgagcaa ccgggcaaag
     9661 gacgcctgcg cgaacactgg cagcttaact gcgatatttt cggtgcacca aatgaaaaac
     9721 ttgccgattt agagatcctc aacttagcaa tcgacctcat gcgcggctac ggcgccaatg
     9781 aatcgcattt caaactttac ctctcacacc gtggcatttt gaacggtgtg ttggggaagg
     9841 agctcaaact accaccagag aaatgggcgg gcgtcgcgcg catcatggac aagcgcgcga
     9901 aaattaaacc cgacgcgtac atcgagatgc tccgtgccga aggtgtgggc gataacgagc
     9961 agcaagagtt agatttattc ttaaacgaaa aaataactta tctaaagaaa aaatctcact
    10021 tagaaggcgc aagctatgtc gtcgaaatgc tgcaaagcct cgaagagatt ggcctgaatg
    10081 catttgtcga gtacgacccg tcggtggtgc gcggctttga ttattgcacg ggccttgtgt
    10141 tcgaggtgtt cgacgcacac cctgacaacc ggcgttcact ttttggcggt ggacggtaca
    10201 acaacctcgt cggcgcattc ggcaaagaaa atttgaacgc agtgggtttt ggtatgggcg
    10261 acgtaacgct cgaagatttt ttgcgtacgc atcaactctt gggcgatgtg ccgcgcaaag
    10321 tggagcttta tatcgcactc atggacgaga aacttctcga acatgcaatg cagcttgcaa
    10381 gtacgcttcg tgcgcgcaat ttaagcgtgg aagtgtcact cggcgcaggg aaactgggca
    10441 aacaattcca agaagcggaa aagaaaggca tcccgcgcgt gttgattttg ggcgaagacg
    10501 aagcgaagtc gggcgtttat agtgtgaaga cgctgacaac cggcgaacaa gtaaaactca
    10561 aagcaactga aatttaagtt ttccccgttg cttttaccgc tacgctacta aactcgtgaa
    10621 aatacgggca atagtcgatt tggcgcacag gctctcagat acttacaata ttgacgtgga
    10681 gcttgcaaaa tcctccatga cgtatgagcg ttaaaaaata tacaggtagc tgccactgcg
    10741 gcgaagttcg ttatgaagta caattagatt taacacaacc ggtcactgcg tgcaactgct
    10801 ctatgtgtgg ccgcagcgga acgtatttga catttgtgtc cgaaaacgat tttgttttga
    10861 aatccggtag cgactcttta caggactatc agtttaataa aaagcatatc caccatttgt
    10921 tttgtaaggt ctgtggaatt aaaccctttg cacgtggtgc tgcgcccgat ggttcatcca
    10981 ctatcgccgt taacgtgcgc tgtttagatg gcatagaatt cgataaactg caagtacaac
    11041 actacaacgg caaatcatta tagtttttca gtcagcggca taattcaaaa acgagacaca
    11101 gtgggtgaaa aaaatgtcct ttaatgccga taacgacgta tatactataa agagggaatt
    11161 atggctaaaa caaatacaag agccaatcaa cggacaacaa atcaagatcg taatacaaaa
    11221 actacacttt cccttgccga taaacttttt gcgatcacaa cacgcaatta tgaaaatagc
    11281 gctgaaaaat ttttaccgaa aaaacataag aataagttag caatccacga ttttcaacgt
    11341 acacataaag aaactctcac aaagcttgaa cccatcacca ctgcttcttt acccattaaa
    11401 gaaaccgaag gcaaactcct cacactcggc cgtaccgttg cacgccgcac catggttgtc
    11461 atggcgcgtg cagcgctttt tatcatcgcc attagcgcgg gactgacact cattagctat
    11521 atcgccgacg attcgattcg catcgaagac gaacgcggaa aaaatgcgct caacaacgaa
    11581 atccgggcca aagaaaaagc ggtctcgctg aacgttatgc gcaagcgcaa aaaaattggt
    11641 gagtttaccg ttgcaaaagg ctcacggttg cgcatcgtaa aagcaaccaa tggcgatacc
    11701 ttccgccccg aagtggcgtt taccggtgaa tcggcagatt tcaaactcaa gtacaaaggc
    11761 aaaacagcgc tcatcgtaac caatggccca ttccatgcaa aagtgaaaat tgcgcaaggc
    11821 gccaacaacg acgtgcattt acgtttcaaa gaaacaggca cgctattgcc cgtaggcgaa
    11881 cctgaattta gcattgaagt cgtcaaaggc gatgttcaaa ttgccgagac cgacgacaat
    11941 gtgtacgaag aatataaaac aggcgagcag gccgtgttca ctctttcttc gagcgattct
    12001 ggcagtgaga atctgtagat aatctaactt taattttcta agtaatcttt atcgaacgtg
    12061 gtccgctctt tgaggcgttg caaaaggtta gcagccatgc gcttcatggt gcgttggggt
    12121 atattcaaaa ttttttctaa atcgttgtat ttgacttgtt caattttttg taagataaaa
    12181 aggttgcgtt ccgaatcgtt gagtgaatcc aagaaatcgt cgatatgctt ttccaattct
    12241 ttttgataga ccttgccgac ttgtatgtct ttttcccatg tgtcgctttc ttgtgtgttt
    12301 tgtaaatcgc gcgttaaacg gcggtgggtg tcgcgcacat aagttaaaat aacgttgcgt
    12361 gcagtcttca cgagccacga ctcgatgcgg tcggttgaaa tatccagatc gggcaacttg
    12421 tcgtaaaaag tgataaacac ttcttgcaat aaatccaacg cttcgtcgta atcgcgcact
    12481 gtacgtgaaa tatagtttaa aacacgattt ttacttttat tgtaaatacg ctggaaatca
    12541 gcctcacgtt gctgccgact ataagccggt ctttcatgga aggatttacc ggtcatagtt
    12601 aataccaatc taaaaaaagg agatacatag tatagagctt gtgaacttgt acgaataaaa
    12661 acaatggacc cccaaggaca gtggcaaaat aaattccaag cattgatcga tgcttttgta
    12721 aacaattttt cacaaaaaag agaacacggc gctgcgctct gtgtatttca aaatggcgag
    12781 aaagttgtcg atgtatgggc gggtatggca gacctcaaaa cgcgcaaaat gtggcaacaa
    12841 gacactctcg tgaccatttt ttcggtgacg aaagccctca ccgcactttg ctttcttatc
    12901 ctcgcactac gcaaaaaatt tgactacgac aaaccggtag cctactactg gccagaattt
    12961 gcactgcgtg gtaaaaataa tatttcctgt cgtttgcttc tcgaacaccg tgcgtcgctc
    13021 tatgccatcg acacaccact tatgttatac gactacgtgg aaaatcccac caaggtgtat
    13081 aacacactga tccaccaaca accacttgcc gtacccggtt ccttacaagg atacggtgcg
    13141 caggcgtggg ggatgtatgc tggcgcgctc tttcaaaaaa ttaccggtga aagtgtggga
    13201 gtattttttg cgcgtgaagt ggcagcccca ttaaaggtgg acgcatttat tggcttaccg
    13261 catgggttgc aaaataaaat ggcaacgatc tatcctgtaa ccgcaattga acggctggta
    13321 ggagtaatcc cggatcttct gtgtggtgac accaccgaag gccgtatcgc tcgtgcgttc
    13381 atttcgggca atacatcgat taagcaggcg tatatgaatc cttccactgg ggacaaagga
    13441 atcgatgtgc tcaacgatcc catccttaga gaaattgagc taccttgggt aaacggtgtc
    13501 agtaatgcac gctctctggc aataattatg gctgtgtttg cgcaaggggg taaagcgtat
    13561 ggtgttacat ttgcggatgc accgctcatg caaacactca tacgcgagaa taagttgcgc
    13621 gacgatttag tgctacaaaa acgcctcggc tggaatttgg gttttctcaa agaagaaaca
    13681 catctttttt ctcccaacac cgaaacgttc ggtcactccg gcatgggtgg ctcactcgca
    13741 tttgccgatc cgaaagttag ggtgtcgttc gcgtacacct gcaataaaat ggacgctaaa
    13801 atccgtccag acaaaacgct cgcactttgc cacgcacttt ataagtgtgt atgaataatg
    13861 aaggtggtta tggccttcgt ggtgcgaaga agtggggccc tacccgtctg aaagagagct
    13921 tttcctcaaa atttactctc cttttcgacc actccagcac tcggggcgca tagttacccc
    13981 caacgatgaa tt
//
LOCUS       DAOMPD010000010        13011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2057_length_13011_cov_2.320161, whole genome shotgun sequence.
ACCESSION   DAOMPD010000010 DAOMPD010000000
VERSION     DAOMPD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 13011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13011
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_13011_cov_2.320161"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLY93_00845"
     CDS             complement(<1..893)
                     /locus_tag="PLY93_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68066.1"
                     /translation="MPIKFLRQLIAQWILKYCFIRYCFFFFFFAGASLFALAELQIAP
                     AYNIPLATMGSYFNNATGVEIHARGTTRFTWLQWIEKRGFYFAAGLAIVPYSMRERQA
                     SKLTVYQLWGGVDYPLPLPYGLTFIPRLGVGAYYGNYLKSGRNENLSIVNPLIHTSFA
                     VQYFLTRELSVELAHGYTFAYMGNPTWFSQAHLSLGVSFKIGVPADKLSPASQLRDDI
                     AQHYDEGDYVRASKEIDALEKITPDDLIVEKYRSLIYQQKKYQEAKKQYAAGKTLRAI
                     ALLRQVTDIPEAQEDLALLRG"
     gene            complement(897..2390)
                     /locus_tag="PLY93_00850"
     CDS             complement(897..2390)
                     /locus_tag="PLY93_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP68067.1"
                     /translation="MNRYVLYSLLSFTACQIDLEREIQQDIDTTQPTVQSSIPQDENV
                     RRLTTLELTFSETIKGGTEVGAYTLAGGGVGTLVITAVDTNSSNARTLHFTGVPHDGT
                     LTLSIAGVTDHVGNPLQNNVLTWQGDGLPKWHYLGRKLSRGFIASNQIRIGSLGDEVV
                     LVYSDEECRANYGFHNLTALAFNVKTKVARALGQACIVGGDTAEPLASNILIIDNKIY
                     FAILDTSTTDKKATVLTFNGSAWVAVGSERFTPAAQTGSSQLSYIDGFLYLTYRDGAT
                     SNYLRVYRKNLSTDDDWIYLDVLPMTDTNPFAAQKLKKYGNQLFVGVRDTACKAPSVR
                     YYDFGSNSYSTKIKLESTSSNTSCDEVQMLESANTPLLAFSIRLNASTRAIRFLKWDT
                     LGWADTNWQEEYATYDTGSTSALNYLDCQLRGTQPFCNYVEGAGFNYTLTYTVVDGWQ
                     HVKSPTETKSAINGGHPGFLVLNDVIYLAVRDKDVGYSDTLSLLSYE"
     gene            complement(2404..4875)
                     /gene="sppA"
                     /locus_tag="PLY93_00855"
     CDS             complement(2404..4875)
                     /gene="sppA"
                     /locus_tag="PLY93_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRP68068.1"
                     /translation="MKKNLVYFFSIVVPLQFLSAQQVVPVQSDALHTNRMIDTPNLPQ
                     KSVATSDDTTSLYFNPAGLGYHALQLGYFYGRNPDNKLDDHMVFLNLLGIAFSTQWRF
                     GDTTQDYARRYTLGMGIINTPFLSIGTSYSWYNSSFAYLDRYAQWDIGFILRPWRRFS
                     LGAVGRAINEPIYRGQIFQTNWDVGVGFRPLPGKYTENLTLSIDTNLSPLTTWQNLSP
                     KFLVEATLWPGNSLYAGYGETNGAFFGLRFAQYISQISLHVSTPSDSGTFMGGGILIG
                     REQYSTGISALGRYLEIRLDDKIDERKQQGFLFLSENFTFLELLRTIETAKSDAQIRG
                     ILITGRSFEGGWGQAEELRHALLSFQQKKPVYAYLENAGNKEYFIASMADRIYMPESA
                     MLDISGLKAETYFVKDLLNKVGVKADFVAIGDYKSAPDRLTRSEPTKFDKEQLEALLR
                     SNVEELKAAILQARLKIKGENLDSLFDRGYYSATRAIGAGLIDEVAYMSDVEEQLTAK
                     TTTAPQWRVNAAEYAKTRFYDETWGEKPSIAVLVLSGEIISGTSRSDGLFTRGSIGSD
                     TIQGVLDDIRDSSNIKGLVVRIDSPGGSSLASDLLWNKIREVKKVRGQDFPIVVSMGD
                     TAASGGYYMAVAGDEILASNTTVTGSIGIFTGKINVKGLYDWLGVKKHTFKTHKSSAL
                     FSETESFTPEERVLIREQLAEFYDLFLQRVADNRGESIERIRPRAGGRVYSGKDARAQ
                     KLVDKQGGLLLALEIVRTKTGIDENYVNVQIYPNEAKDILALGDPKKLLLPSILREAT
                     RIITQTETIKNEKMLFLLPYDLTIR"
     gene            complement(4901..5446)
                     /locus_tag="PLY93_00860"
     CDS             complement(4901..5446)
                     /locus_tag="PLY93_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68069.1"
                     /translation="MLQKNTLIVFALFFFAPLLAAENGKKGMDTKGVKEYVHRKDRFS
                     FFYPETWTIDTTKKDVSVVVVDSKRNAAVSVHTQYPISGKKACQLLQDFEKTNPSINF
                     NLIPEAQQHATADELRFVHATDSCIGVYKYVAKNTELLQGVVVYIRGAKAWFVTQLLV
                     APPQSQYLDIVGSIAKSFRLQ"
     gene            complement(5453..6451)
                     /gene="gap"
                     /locus_tag="PLY93_00865"
     CDS             complement(5453..6451)
                     /gene="gap"
                     /locus_tag="PLY93_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRP68070.1"
                     /translation="MIKIGINGFGRIGRNTFRAAWGRNDVEVIAINDLTDTATLAHLL
                     KYDSIVGVWNHDVTYDKENIIVDGKKIHATAIAKLEEQKWGAFGCDLVIESTGRFTDA
                     TVAKTHIDKGGAKAVIISAPATNEDVTVVLGVNEKSFDKSKHKIISNASCTTNCLAPV
                     AKVIMENFGIKKGLMTTIHSYTNDQQILDLPHKDLRRARAAAQNMIPTSTGAAKALAL
                     VIPELKGKLDGISIRVPTPNVSVVDVTLETEKPTTKEAVNAALKSAAEGPLKGILGYT
                     DAPLVSNDFRGNPLSSIVDGQMTTVIQDNMIRVLTWYDNEWGYSNRVVDLAALVGK"
     gene            complement(6715..7791)
                     /locus_tag="PLY93_00870"
     CDS             complement(6715..7791)
                     /locus_tag="PLY93_00870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP68071.1"
                     /translation="MNFNESALSYTLIDKNRRMMKMRISFVVVFFSCFVFCAGKSTRT
                     PQGTERGLARAQANVSTFPEGADKSLPQVKKYTHEDITFLAWSKDFGRGHAVLFEIES
                     TASLSNLSLFVDGKALPLIQGDGCLFALFANSPESTLKINKVDIHRGGKKLVAFDVPV
                     VAYKYPVAKHKLNVTLTSNTTKPMSEETKQFIAQSWQKKKKAFVSASENYFSSVLRYP
                     RDAHKITSPFHIKRVYERFKMVKGKRKSLKGRVSYHGGTDLKGLTGAPIFAVADGVVN
                     LAEHLYYEGNITLIDHGYGVMTGYMHQSVILVKQGERVKAGQLIGKTGATGNVTGPHL
                     HIFLLIHGVKTEALSLLALPLRPK"
     gene            7829..9529
                     /locus_tag="PLY93_00875"
     CDS             7829..9529
                     /locus_tag="PLY93_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRP68072.1"
                     /translation="MLRFILLLILFPLRFLFFWYVKLRSIHRGLVLTITLKGNYSEAP
                     LSHGLWAYLKPSKDRFYLLALDLAAALHAAEQKKIRLKHVRVLLEENDLGWAQAWEIR
                     DLLQQFRQLGIEVSAYLLVSDRTAVFIASAATTTAAPESAAFDFSPFTGESIFLQNLF
                     SKVGVRPQFLSVGDFKSAAEIFTKTKMSPAARKQTEELLSDIETTFVTAIEEKMKKKL
                     AKSERTIQDTASAKKLGFIDQTCSVSEFKDTPETDALRSVDAYELSQIILRKNFRLFN
                     FKKLKRVALVTAEGNIIESAQPRAGTINWADYAEVGETLKENDFAAMLLRVNSPGGSA
                     LVSQLLWREWMLSTGNIQSVVESEEKEDEKKKAIPIFVSQGNVAASGGYYLSATANRI
                     FSTPLSITGSIGVVGGKFNVTPLLQKLGVTTDRAPLKNPSPLFSPYSDFSKENKKALE
                     KNMLSIYEQFLRDIAVGRRTTVKSIIPHASGRVFSGQRAQTHALVDNLGGITHTLRAL
                     REELKLKPSENIELMILPAIKESLFNRGALPMGLQRLLAWSDFSKFGVYTIEPRFLFL
                     "
     gene            9590..11368
                     /locus_tag="PLY93_00880"
     CDS             9590..11368
                     /locus_tag="PLY93_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68073.1"
                     /translation="MALDTPPFYINLSPTEFREFAHNAGLLEDEYIVLEAYAVYAREN
                     DTLSFSAARITDSLVARGEEENIPKLRTLPNVRRLMTQTAYTLRDKNFLTMDIENGEI
                     LKIYLIDPIETLLSDYYLQMQSNSTMPFPEDDLLQKYITATNTVEVLLSESGGGTDER
                     LQEEKRTIRLRFNESIPPVYVTSKTLDPLLDACFLKLKYVILNAAQNRLGEDLVKDMQ
                     HIMPQKTTTIDRINRILSRTESEAPLYIVNLANRLAAYFTIDKDKKGMMTLIQAARII
                     EAFKGYEAWIEAEKNNKDKLKENALKLASRMADFPELLTRSDIAQKIIRGAVGLENLS
                     SSTPTNEVDALVNLMLADFTVFQAEERELLPMIVKVKISDEDFYIHREAVLIFFESER
                     KRVRDELLARFRKMWYALMLRNESRSAMEFDEFFAEDVQKYIKNHEPVFSALLVNPQL
                     IVNAFHVVSRHPINASLQETYFVFGNRVIFRPLHSLLNLSRREILSRIRSELPFLFRY
                     PFLRWLLSIFGIGSANAPPPVEHAESEPVTHGQTVLQDTEWHRILTMVETKLIGEKSA
                     TEVMRKLQDMWNQKLGEARKQMDEIQ"
     gene            11365..11532
                     /locus_tag="PLY93_00885"
     CDS             11365..11532
                     /locus_tag="PLY93_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68074.1"
                     /translation="MTRKLPEVSQNFMADEVINAASQIARRIGNDSSDQKALEQFTQL
                     GLLEELKVIHG"
     gene            11553..12083
                     /locus_tag="PLY93_00890"
     CDS             11553..12083
                     /locus_tag="PLY93_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRP68075.1"
                     /translation="MIEELSSLKGKLLVASSALLDGYFNQSVILIIEHDSRGTLGITL
                     NNPLPGDRGRKHPLFKGGPVDTDHRSLLHCAAHLTSNAAVVDNVFFENSDDLLDTLAE
                     KDEPYRRFAGYAGWGTGQLEYELHTKSWILAEARADLIFYHVEVDLWRQCLAEKGGLF
                     RYFARTHRNILLNGTL"
     gene            complement(12063..>13011)
                     /locus_tag="PLY93_00895"
     CDS             complement(12063..>13011)
                     /locus_tag="PLY93_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68076.1"
                     /translation="PVYVVCIWLGVQWWASWYPGFEPGGGGYVVQRLLSTKNENHAVG
                     AAILFNILHFVVRPWPWIITALAAAVVLPVGSDKEMLYPQAIVAWLPPGLKGLLIASF
                     LAAFMSTIATHLNWGASYIVNDVLQGTRFLPQSDKGKIWFSRIVILFLAVGAVGVSQL
                     MTSVSQGWELILMFSAGTGPVYLLRWYWKKITAASEIVAMGASFIFSLVAASFAWPQE
                     LRLAFVALCATVSWVLVTLLIAQEKSSHLKKFEALVRPSPWKLREVLVFIAAVVTIYS
                     AFFALGELLKGNYDWAALHGILVAVFSFLISRLLQRTVE"
BASE COUNT         3532 a         3247 c         2818 g         3414 t
ORIGIN      
        1 tgtccacgca acaacgctaa gtcttcttgc gcctcgggga tgtcggtgac ttgccttaaa
       61 agcgcaatgg cgcgcaacgt tttaccggcg gcgtattgtt tcttcgcctc ttggtatttt
      121 ttctgttgat agatgaggct tcggtatttc tcaacgatca aatcgtcggg tgtaattttt
      181 tctaacgcat cgatttcttt acttgccctg acgtaatccc cttcgtcgta atgctgcgca
      241 atgtcgtcgc gcaactgcga tgcaggactt agcttatcgg cgggcacacc aatcttgaaa
      301 ctaaccccca gcgaaagatg cgcttggcta aaccaagtgg gattacccat gtaagcaaac
      361 gtgtaaccgt gcgcaagctc taccgagagc tcgcgcgtta aaaaatattg tactgcgaaa
      421 gaagtgtgta tgagaggatt cactatcgat aaattttcat tgcgacccga ctttaagtaa
      481 tttccgtagt acgctcccac tcctaagcgc ggtataaacg tcagtccata gggcaaaggg
      541 agcggatagt cgacgccgcc ccacagttgg taaacggtca gtttgcttgc ttgccgttca
      601 cgcatactat aaggaacgat cgctaaacca gcggcaaaat aaaaaccacg cttctcaatc
      661 cactggagcc acgtaaaccg cgttgtcccg cgcgcgtgaa tttcaacccc cgtcgcgtta
      721 ttgaagtaac tccccatagt tgcaagcggt atattgtaag cgggagcaat ttgtaactca
      781 gcgagtgcaa aaagagaggc gcctgcgaaa aagaaaaaga aaaagcaata tcgaataaaa
      841 caatacttca aaatccattg agcgataagt tgccttaaga actttattgg catgcgttat
      901 tcataactta aaagcgaaag cgtgtctgaa tatcccacgt ctttatcgcg cacggccaga
      961 taaataacat cgttcagcac gagaaaacca gggtggccgc cgttaatagc tgatttagtc
     1021 tctgtagggg atttcacatg ttgccacccg tctaccacag tatacgtcaa cgtataatta
     1081 aaaccggcgc cttctacata attacagaaa ggttgagtgc cgcgcaattg acagtccaaa
     1141 taattaagcg ctgaagtgct acctgtgtca taagtggcgt attcctcttg ccaatttgta
     1201 tctgcccacc ccaaagtgtc ccatttcaaa aaacggatag cgcgagtgga agcgtttaaa
     1261 cgtatcgaga atgccaatag cggagtattt gcgctttcaa gcatttgcac ttcgtcgcaa
     1321 gacgtatttg acgatgtgct ctctagttta atttttgtac tataactatt gctaccaaaa
     1381 tcataatagc gtacagaggg agctttacaa gccgtatcac gcacccccac gaatagttga
     1441 ttcccgtact tttttagctt ttgcgcggca aaggggttgg tgtcggtcat cggcaacaca
     1501 tctaaataaa tccagtcatc gtctgtcgaa agattttttc gataaacgcg tagatagtta
     1561 ctcgttgccc catcacggta tgtcaaatac agaaatccat cgatgtacga aagttggcta
     1621 ctgcccgttt gtgctgctgg tgtaaaccgt tcgctcccca ccgcaaccca agcggaaccg
     1681 ttgaaagtca gtaccgttgc ttttttgtcg gtagtgcttg tatccaaaat agcaaaatat
     1741 attttattat ctataattag tatattactc gccaatggct cggcagtatc tccacctaca
     1801 atacaagctt gtcctaacgc gcgggctact tttgttttta cgttaaacgc cagtgcggtt
     1861 aagttatgaa atccataatt ggcgcggcat tcttcgtcgg aatagacaag aacgacctcg
     1921 tcgcctaaag aaccaattcg aatttggttt gaagcaataa acccacgcga taatttacgt
     1981 cctaaataat gccatttggg taaaccatcc ccctgccacg tcagtacatt attttggaga
     2041 ggattaccca cgtggtctgt tacaccggca atactcaaag taagagtgcc gtcgtggggg
     2101 acacctgtaa aatggagagt gcgtgcattg ctactattag tatccactgc ggttatcaca
     2161 agcgtaccga cgccaccacc cgccaacgtg tacgccccca cttctgtgcc gccttttatt
     2221 gtttcagaaa aagtaagttc gagcgtcgtt aaacggcgta cgttttcatc ttgaggaatt
     2281 gagctttgga cggtgggttg agtcgtatcg atgtcttgct gaatttctct ttccaaatca
     2341 atttggcagg cagtaaatga aagaagcgag tatagtacat acctgttcat caacgttcat
     2401 ccgttaacga atggttaagt cgtacggcaa caaaaaaagc attttctcgt ttttaatcgt
     2461 ttccgtctgt gtaataatcc gtgtcgcctc gcgtaagatg ctcggtaata ataatttttt
     2521 gggatcgccc aacgctaaaa tatctttagc ttcgttgggg tagatttgaa cattcacata
     2581 attttcgtcg ataccagttt tagtgcgcac gatttctaac gccaaaagaa gcccaccctg
     2641 tttatcgaca agcttttgcg ctctggcgtc tttgccgctg taaacgcgac caccggctct
     2701 gggacgaatt ctctcaatcg attcgcctcg gttatcggcc acgcgttgca gaaataaatc
     2761 ataaaactcg gcgagctgtt cccgaatgag cacccgctcc tcgggggtaa agctttccgt
     2821 ttcgctgaaa agcgcgctac ttttatgcgt tttgaatgtg tgttttttca ctcccaacca
     2881 atcgtaaagc cccttcacgt taattttacc ggtgaagata ccaatcgaac ctgtgacggt
     2941 ggtattgctc gctaaaattt catcacccgc caccgccatg taatacccgc ccgaggcggc
     3001 ggtgtccccc atcgacacca caatcggaaa atcttgccca cgcactttct tgacttctcg
     3061 aattttattc cacaataaat cagaggcaag gcttgagcct cccggcgaat cgatacgcac
     3121 aacaaggcct tttatattac tgctatcgcg gatgtcgtcc aacactcctt ggatggtgtc
     3181 tgagccaatg ctaccgcgtg taaataatcc gtccgaacgc gaggtccctg aaataatttc
     3241 acccgagaga accaacaccg caatcgacgg tttttccccc cacgtttcgt cgtagaaacg
     3301 ggtcttcgcg tactcggctg cattcacacg ccattgcggc gccgttgtcg ttttagcagt
     3361 gagttgctcc tcgacgtccg acatatatgc cacctcgtct atgagccctg caccaattgc
     3421 ccgcgtcgcc gaataataac cacggtcgaa aagagaatcc agattttctc ctttgatttt
     3481 aagccgcgct tggagaatgg ccgctttgag ttcttctaca ttcgagcgca aaagcgcttc
     3541 caactgttct ttatcaaatt tagtaggctc gctacgcgtc aatcgatcgg gcgccgattt
     3601 ataatcgcca atcgcgacaa agtcggcttt aactcctact ttattgagca aatctttgac
     3661 gaaatacgtt tctgctttga gccctgaaat atcgagcatc gccgattcag gcatgtaaat
     3721 tcggtcggcc atgctggcga taaagtattc tttattaccc gcattttcca aatacgcgta
     3781 caccggcttt ttttgttgaa aactcaaaag cgcgtggcgg agttcctccg cttggcccca
     3841 gccgccttca aaactacgcc ccgtaatcaa gatacctcga atttgtgcgt cgctcttggc
     3901 cgtttcaatg gtgcgcaaga gttcaaggaa agtaaaattt tcagaaagga aaaggaagcc
     3961 ttgctgtttg cgttcgtcga ttttatcgtc gaggcgtatc tccaaataac gccccaatgc
     4021 gctaatacca gttgagtatt gttcgcgccc aataagaata cctcccccca taaatgtgcc
     4081 cgaatcactg ggagtgctga catggagtga aatttggcta atatactgtg caaatcgaag
     4141 tccaaaaaat gcgccgttcg tttcgccata ccctgcataa agactattac ccggccacag
     4201 cgtcgcctcg actaaaaact tgggcgataa attttgccat gtggttagcg gtgaaagatt
     4261 cgtgtcgata gacaaggtca aattctcggt gtatttacca ggaagcggtc taaatccaac
     4321 accgacatcc caattggttt gaaaaatttg acctctatat atcggttcgt tgatagctcg
     4381 ccccaccgca cccaaagaaa acctgcgcca aggacgcaga ataaagccaa tatcccattg
     4441 agcatagcgg tcgagataag caaacgatga attataccac gaatacgatg taccaattga
     4501 aagaaaaggg gtattgataa tccccatccc caaagtatag cggcgtgcat agtcttgggt
     4561 tgtgtcgcca aaacgccatt gtgtagagaa agcaatcccc aacaaattca agaagaccat
     4621 gtgatcgtct aatttattat cggggttacg cccgtaaaaa taccctaact ggagtgcatg
     4681 ataccccaac ccagcggggt taaaataaag gctcgtcgtg tcgtcggaag tcgccacaga
     4741 tttttgtggt aaattgggtg tgtcgatcat acggtttgta tgcaacgcat cgctctgcac
     4801 aggtacgact tgttgtgcag ataaaaactg tagagggaca actatgctaa aaaaatagac
     4861 gagatttttt ttcattcttt atttatactc gactgtatct tcactgcaag cgaaacgact
     4921 ttgcaatact gccaacaata tctaagtatt gactttgggg cggagcaaca agtagctggg
     4981 tcacaaacca cgcttttgct ccacgaatgt agacgacaac tccttgcaaa agttctgtat
     5041 tcttagcgac atacttataa acgccaatac agctatcggt ggcatgcaca aaacgcaact
     5101 cgtccgccgt tgcgtgttgt tgcgcttccg gtattaaatt aaaatttata gatggattgg
     5161 tcttttcaaa atcttgaaga agttgacacg ctttcttacc cgatatggga tattgtgtgt
     5221 ggacactcac tgcagcattt cttttagaat caactaccac cacggaaaca tcctttttcg
     5281 tggtatcgat agtccatgtt tcaggataga aaaatgaaaa acgatccttc cgatggacat
     5341 attctttcac tcctttcgta tccatcccct ttttgccgtt ttcggcggca agaaggggag
     5401 caaagaaaaa aagggcaaac acgatgagtg tgttcttttg aagcactcgt gcttatttac
     5461 ccaccaaagc cgctaaatcc acaacgcgat ttgagtaacc ccattcgttg tcataccagg
     5521 tcaacacacg gatcatgttg tcctgaatga cggttgtcat ttgaccgtcg acaatcgacg
     5581 ataaggggtt ccctctaaag tcgttagaaa ccagcggtgc atcggtgtat cccaaaatgc
     5641 ctttgagcgg gccttcggct gccgacttta acgccgcatt caccgcttct ttggtggttg
     5701 gtttttctgt ttcaagcgtt acgtcgacta ctgaaacgtt gggtgtcggc acacggatgg
     5761 aaataccatc gagtttacct ttcagttcgg ggatgaccaa cgcgagcgct tttgccgcac
     5821 cggtcgatgt gggaatcatg ttctgcgctg cagcacgcgc gcggcgtaaa tccttgtggg
     5881 gtaaatccaa aatttgttgg tcgttggtat agctgtggat tgtcgtcatt agtccttttt
     5941 tgataccaaa gttttccata atgactttag ccaccggtgc aaggcagttt gtcgtgcacg
     6001 atgcgtttga aatgatttta tgtttagatt tatcaaaact cttttcattg acgcccaaaa
     6061 cgacggtcac gtcttcattg gttgctggcg ctgaaataat taccgctttc gctccgcctt
     6121 tatcgatgtg cgttttggcc acggttgcat cggtaaagcg gcctgtcgat tcgatgacta
     6181 aatcgcaacc gaatgcgccc cacttttgct cttcgagctt agcgattgcc gttgcatgga
     6241 tttttttacc gtcgacgata atgttttctt tatcgtaagt cacgtcgtga ttccacacgc
     6301 cgacaatgct gtcgtatttc aaaaggtgtg cgagtgttgc ggtatcggtt aaatcgttaa
     6361 tcgcaatcac ttcgacatcg ttacgccccc acgctgcgcg gaacgtattg cggccaatac
     6421 ggccaaaacc attaattcca atttttatca tggacttggt taaaaatcaa aaatggggtg
     6481 tcgagtgaaa tgtgattact gtggccaggc cggaactcgc tgaggcaaat tttttgcaca
     6541 ttttgaagcg ctattttgca caaattcctc cgcaaattgt accaatttgc tctgcacggg
     6601 gcgaagacgc ccgaatttgt gcaaaatttc gcatttttga gcgaaccgcg cttcaaaatg
     6661 tgcgaaaaat ttgctcttta tcttggcttc gcgagttccg gcctacccac attactattt
     6721 agggcgcagt ggcaatgcga gtaaactcag tgcttcggtt tttacgccgt gtatcagcag
     6781 aaaaatgtgc aagtgcggcc cggttacgtt gccagtcgct cccgttttgc cgatgagttg
     6841 gcctgcttta acgcgctcgc cttgttttac caaaatcacg ctctggtgca tatatcccgt
     6901 catcacgcca tagccgtggt cgatcaatgt gatattgcct tcgtaataga gatgctcggc
     6961 taaattcacc actccatcgg cgacagcaaa aatcggcgca ccggtgagtc ctttaagatc
     7021 ggtgccgccg tggtacgaaa cacgcccttt gagcgatttc cttttgccct tgaccatctt
     7081 aaagcgttcg tacacccgtt taatatggaa aggcgaagta attttatgcg cgtcgcgggg
     7141 gtagcgtaaa actgaagaaa aataattttc cgatgctgaa acaaacgctt tctttttttt
     7201 ctgccaactt tgcgcaataa attgttttgt ttcttcggac ataggcttgg ttgtatttga
     7261 agtgagagtc acattgagtt tatgctttgc cacgggatac ttatacgcga caacagggac
     7321 gtcaaacgcc accaactttt ttccaccacg gtgtatatcc accttgttga tttttaatgt
     7381 gctttcggga ctattggcaa aaagagcaaa aagacaacca tcgccctgaa tcaacgggag
     7441 tgctttaccg tcgacaaaaa gcgataaatt ggaaagcgat gctgtcgact caatttcaaa
     7501 aagtaccgca tgcccacggc caaaatcttt cgaccacgcc aagaaagtga tgtcttcgtg
     7561 cgtgtatttt ttaacttggg gcaacgattt atccgccccc tcggggaaag tagacacatt
     7621 cgcctgcgcg cgtgcaagtc ccctttcagt accctgcggt gtgcgtgtac ttttacccgc
     7681 acaaaaaaca aaacacgaaa agaatacgac gacaaacgaa atccgcattt tcatcatgcg
     7741 tctatttttg tcgatgagcg tgtacgacaa ggcagattca ttgaaattca cttttcggcc
     7801 tgtttcgctc gtttagatac tacactcgat gcttcgtttt attcttttat taatattatt
     7861 cccgctacga tttctcttct tttggtatgt taaattacgc tcgatacacc gtggtttagt
     7921 actcacgatt acacttaaag ggaattattc cgaagctccg ctatcgcacg gcttatgggc
     7981 ataccttaaa cccagcaaag accgcttcta tcttttagcg ctagacttgg ccgccgctct
     8041 ccacgcagca gagcagaaaa aaatccgcct aaagcacgtc cgtgttctct tggaagagaa
     8101 cgatttaggc tgggcgcaag cttgggaaat acgcgattta ttgcagcagt tccgccaatt
     8161 aggtatcgag gtgagcgcat accttttggt aagcgatcgc accgccgttt ttatcgcgag
     8221 cgccgctacc accaccgctg cgcccgaaag cgcggcattt gatttctcgc ctttcaccgg
     8281 ggaatcgatt ttcctgcaaa acctttttag taaagtggga gtgcgccctc aatttttatc
     8341 ggtgggagat ttcaagagcg ccgctgaaat tttcaccaaa acaaagatgt cccctgctgc
     8401 acgcaagcaa accgaagaac tcttgtcgga catcgaaact acttttgtga ctgcgatcga
     8461 agaaaaaatg aaaaagaagc tcgccaaaag cgaacgcaca atacaagata ctgcaagcgc
     8521 aaaaaaactg ggctttatcg accaaacttg tagcgtgagt gaatttaaag acaccccaga
     8581 aaccgacgcc ttgcgttctg tcgacgcgta tgaactatcc caaattattc tacgtaaaaa
     8641 ttttcgcctt ttcaatttca aaaaattaaa gcgggttgcg ttggtcaccg ccgaggggaa
     8701 tatcatcgaa agcgcacaac cccgcgcagg caccatcaac tgggcagact acgccgaggt
     8761 gggtgaaacc ctcaaagaaa atgattttgc cgccatgctc ttgcgcgtca atagcccggg
     8821 tggttctgcg ctcgtctcgc aactcttatg gcgcgaatgg atgctcagca cgggcaacat
     8881 ccaaagcgtc gtcgagagcg aagaaaaaga agacgaaaag aaaaaagcga ttcccatttt
     8941 cgtttcgcaa ggcaatgttg cggcatcggg ggggtattat ctttcggcga cagcaaatcg
     9001 cattttttca acgccgctct ctatcacggg gtctatcggc gtcgtaggcg gaaagttcaa
     9061 cgtcactccg cttttacaaa aattaggcgt cacaaccgat cgtgcgccgc tcaaaaaccc
     9121 atcccctctc ttttctccct attctgattt tagcaaagaa aataaaaaag cgctcgaaaa
     9181 aaacatgctc tcgatttacg aacaattctt acgcgacatc gccgtcggtc gaagaacaac
     9241 ggtaaaatcg attataccgc atgcttccgg tcgcgtcttt tccgggcaac gcgctcaaac
     9301 tcacgcgttg gtcgacaact tgggcggcat cacgcacacg ttgcgcgctt tgcgcgaaga
     9361 attgaagctt aaaccttctg agaacattga actcatgatt ctaccggcga tcaaagaatc
     9421 cctctttaat cgtggcgcgt tgccgatggg tttgcagcgc ctattggcgt ggagcgattt
     9481 ttcaaagttc ggtgtctata caatcgaacc gcgcttttta tttttatagg acacgcttta
     9541 ataccatgaa ctctttaatt tttcagccat taggtcgaaa atagaaagaa tggcattaga
     9601 cacaccgcca ttttatataa atctatcgcc aactgagttt cgtgaatttg ctcacaatgc
     9661 cggcctttta gaagacgaat atatagttct cgaagcgtat gccgtttacg cacgcgagaa
     9721 cgatacgctt tcctttagcg cggcgcgaat tacagactct cttgtagcgc gcggcgaaga
     9781 agaaaacatt cccaaattac gcactctccc caacgtgcgc cgattaatga cacaaaccgc
     9841 atacacactg cgcgataaaa atttcttaac aatggacatc gaaaacggcg agatattaaa
     9901 aatttatctt atcgatccca tcgaaacatt actctccgat tattatctgc aaatgcagtc
     9961 aaattcaacc atgccttttc cagaagacga cttattacaa aaatacatta ccgcgaccaa
    10021 caccgttgaa gttttactca gtgaaagcgg tggcggtacg gacgaacgtt tgcaagaaga
    10081 gaaaagaaca attcggttgc gctttaacga atccatccct cctgtttatg taacttctaa
    10141 aactctggat ccccttctcg atgcttgttt tctcaaactc aaatacgtca ttctcaacgc
    10201 cgcacaaaat cgcctcggtg aagatttggt caaagatatg cagcacatca tgccacaaaa
    10261 gacaacgaca atcgaccgca ttaatcggat tctctcgcgc acagaaagcg aagcccctct
    10321 ttatatcgtc aacttagcaa accgtttagc cgcatacttc actatcgata aagataaaaa
    10381 aggaatgatg acgcttatcc aagcggcgcg cattatcgaa gctttcaagg gttatgaagc
    10441 gtggatcgaa gccgaaaaga acaataaaga taaattaaaa gagaatgctt taaaactagc
    10501 cagtcgcatg gcagacttcc ctgaactttt aacccgctct gacattgcac aaaaaattat
    10561 acgtggggca gtaggacttg aaaacctgtc gtcgtctacg ccaacaaacg aagtcgacgc
    10621 cttggtgaat ctcatgctcg ccgatttcac tgtttttcaa gctgaagaac gcgaactttt
    10681 acccatgatc gttaaagtaa aaattagcga cgaagatttt tatatccacc gcgaggcagt
    10741 gcttattttt tttgagtcgg agcgaaagcg tgtgcgcgac gaacttttag cgcgctttcg
    10801 taaaatgtgg tacgcgctca tgctgagaaa cgaaagccgc tcggcgatgg agttcgacga
    10861 attttttgcc gaagacgttc aaaaatacat caagaaccac gaacctgttt tttctgcgtt
    10921 gttagtcaat ccccaactca tcgtgaacgc tttccacgtt gttagccgcc accccatcaa
    10981 cgccagtttg caagagacgt attttgtttt cggtaatcgt gtcattttta gacccttaca
    11041 ctcgcttctt aatttaagcc gtcgtgaaat tctctcccgt attcgcagcg aactaccttt
    11101 tttatttcgt tacccttttc ttcgttggtt actttctatt tttggtatcg gctccgcaaa
    11161 cgctccgcct cccgtagaac acgcagaaag cgaaccagtc acccatggtc aaacagtcct
    11221 acaagatacc gaatggcacc gtattttgac catggtcgaa acaaaactca tcggcgaaaa
    11281 aagcgccacc gaggtcatgc gaaaattaca agatatgtgg aatcaaaaat taggcgaagc
    11341 gcgcaaacaa atggacgaga ttcaatgaca cgcaaacttc ccgaggtgtc acaaaacttt
    11401 atggcggacg aagtcatcaa cgcagcatcg caaatcgcgc ggcgcatcgg caacgattcg
    11461 tcagatcaaa aagcgcttga acaattcacc caactgggtt tactcgaaga gctgaaagta
    11521 attcatggct aaagaggttg tgctctactc gtatgattga agaattgtca tcactcaaag
    11581 gtaaattact cgtcgccagc tctgcgcttc tcgacggcta tttcaatcaa agcgtcattt
    11641 tgatcattga acacgattca cgaggtactt taggtattac gctgaacaat ccgctaccag
    11701 gcgatcgagg tcgaaagcac cctctattca aaggcggccc tgtcgatacc gatcaccgtt
    11761 cgcttttgca ctgcgccgcg catttaacca gcaacgccgc cgtcgtcgac aatgttttct
    11821 ttgaaaattc cgacgactta ctcgacacac tcgccgaaaa agacgaacct taccgccgct
    11881 tcgcagggta cgccggttgg ggcaccggtc aacttgaata cgagctccac accaaaagtt
    11941 ggattctcgc cgaagcgcgc gccgatttaa ttttctacca cgttgaggtg gacctgtggc
    12001 ggcaatgcct cgcggaaaaa ggcgggcttt tccgctattt cgcgcgcacg caccgcaaca
    12061 ttttactcaa cggtacgctg taaaagacgg ctaatcaaaa aactgaatac tgctaccaaa
    12121 attccgtgca gtgctgccca atcgtaattt cctttgagta gttctcccaa tgcaaaaaaa
    12181 gcagaataga tagtcaccac cgctgcaata aacaccaata cctcgcgcag cttccatggt
    12241 gatggccgta ctagcgcttc aaacttttta agatgagagc ttttctcttg tgcgattaag
    12301 agcgtaacca aaacccacga caccgtggcg caaagagcca caaacgctaa acgtaattct
    12361 tgcggccaag caaaagacgc cgccacaaga gaaaaaataa acgacgctcc cattgccaca
    12421 atctcgctcg ctgccgttat ttttttccaa taccaacgta agagatacac cggccctgta
    12481 cccgcagaga acatgagaat gagttcccat ccttggctca ctgaagtcat gagctgcgac
    12541 acccccacag cccctacagc taaaaataaa attacaattc gggaaaacca aatcttgcct
    12601 ttatcgcttt gcggtaaaaa ccgcgtgcct tgcaaaacgt cgttcacaat gtaggatgca
    12661 ccccagttta agtgagtggc gattgtagac atgaacgctg ctaaaaaaga agcgatgagt
    12721 aaccctttaa gcccgggcgg caaccaagcc acaatcgctt gtggatagag catttcttta
    12781 tcgctaccga cagggagaac cactgctgcg gcaagcgcgg tgatgatcca cggccaaggg
    12841 cgtacgacaa aatgaagaat gttaaaaaga atcgctgcac ccaccgcatg attttcattt
    12901 ttggtcgata aaagccgctg caccacataa ccaccgccac cgggctcaaa ccccgggtac
    12961 cacgacgccc accactgcac acccaaccaa atacacacca cgtagaccgg c
//
LOCUS       DAOMPD010000011        12828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2105_length_12828_cov_2.670164, whole genome shotgun sequence.
ACCESSION   DAOMPD010000011 DAOMPD010000000
VERSION     DAOMPD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2105_length_12828_cov_2.670164"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="ychF"
                     /locus_tag="PLY93_00900"
     CDS             <1..1039
                     /gene="ychF"
                     /locus_tag="PLY93_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRP68077.1"
                     /translation="TKSHSAEAQNYPFCTIDPNVGVVAVPDARFDFLNEIVKPKNSVP
                     VAVEFVDIAGLVKGASQGEGLGNKFLDHIRKVNAILHVVRCFQDENVTHVSGNVSPRS
                     DVEIIELELILADADQIEKRLQNLLKKKKSGDKEASEQSAILERVLEYLKQGKPASAV
                     GLTADEKFAIKDLALLSLKPVLFIGNIDEALLATPEKSPEFVELTKLAHERGQEAIPI
                     SGKIEAELGQLSPDEEKEFLKDLGLTEPGLNRVIREAYKLLGYITFFTAGEVEVRAWN
                     VIAGSTAPQAAGEIHSDIEHGFIRAEVTPFDAVKEFGSQKKAQEAGKMRLEGKEYIVA
                     DGDVVYFRFNV"
     gene            1069..1662
                     /locus_tag="PLY93_00905"
     CDS             1069..1662
                     /locus_tag="PLY93_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68078.1"
                     /translation="MRISFGVVYFLVAALFFSLFALTIAVEKNPLPAEALAFPAFSEN
                     YRAKILDGDYEIWSEEKIERWCSAQGLAVTSDNPQGGLQYKRAAVLIAPGLTFSLTRP
                     VTEKGSEFANGWLLNLDIAAIRPRTGVYTNFLEADVFVDGLFFRKILQGGNRKEESPI
                     QIQIPHIASREGRIRVEIRLANHPRNFLFLYDAYLSR"
     gene            complement(1874..3127)
                     /locus_tag="PLY93_00910"
     CDS             complement(1874..3127)
                     /locus_tag="PLY93_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68079.1"
                     /translation="MKGAAGIIILAIFLMTAWGSVSAAPRKGTQPNRAAAKKNTKPLP
                     TKQQKTLPTQVAPTGKEEKEIAAPSPASKLPKREYKNLDISKNPTLGTFTIGFSAGLY
                     NFTTRTENSTLKTTRTYTLDSLDITALFGLDLAFRLGKDSEDNHDSKTGFHYYRSNRG
                     IFTDFEIGMKTQKTEEKPTRFNYTESSPSGTTTIPVDTGYDPTKGFSAIKQTNLTNAG
                     ISQQLRQSVGSSATEVTMVYASAYYHLTPLNFLLNFGSAFRWFDASVGPSLRVWYYRD
                     YADPVRQAVRNDDLTYSTFMIAYRQYIQFHPKVRLSTRFYFPALTYFTQMAKSGRFNE
                     NEYILNATLDIYALRFNEIGLIISVGYEGHWWYTNPYSSDRFVRTGFVNDSTTDAHYA
                     GYEHKDRTSWEAFATIAIDYHFMGN"
     gene            complement(3124..3600)
                     /locus_tag="PLY93_00915"
     CDS             complement(3124..3600)
                     /locus_tag="PLY93_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68080.1"
                     /translation="MVKLKSALVLFIALTFLLSCAFTEVAEEHSNPLDPIYDSGSFRL
                     SLVGEDTGTSAKLSWGHIFYKDAKGSVSELTEDEIKVSGTAQVLFKNTTPSAADITTV
                     KAAGALGGYTPVHTIPNAKESTQMVTGSYKGYYIIRFDYKYDGKTGRLYSNFVAVQ"
     gene            complement(3594..4460)
                     /locus_tag="PLY93_00920"
     CDS             complement(3594..4460)
                     /locus_tag="PLY93_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68081.1"
                     /translation="MPSIKNTALLLFFVFIPSFVDAAGGKSGETLGLDKEYFSTQKVV
                     RVRDAIGKGRSQEIAIYNAKIDAHNKALARSISSFRHMLTTSKDMQIADEQLITDVNA
                     KVIDEARCSDPKWEHGIDFSPFYVAVMDCQVTVSFFDLDYFTTEIIKTAEGACIRSIV
                     LSGWGQFYNKNYVTGTAMALITYGSIGYGYSRETKIAQAQKDYEAALTPGDAEKKYKA
                     MRNNRDVARTMYVIGALTWAYSVWDAFEDRERADDVLYQVHQKYFAGKLNYTPQLSFF
                     QEFMMKNTRPKW"
     gene            complement(4448..5239)
                     /locus_tag="PLY93_00925"
     CDS             complement(4448..5239)
                     /locus_tag="PLY93_00925"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRP68082.1"
                     /translation="MKYTQHFILLIALVAVFPLVAGEYTNSFKVKISTEDGVGESLNN
                     DPVEAFNNAVDQAMKVGTSLGESELVGRSESKDFVLQDTWVKQEAHLQVTDVQITNYR
                     FSRPAGEPLRVNLSATMQLDYVDLPKFMEDYEKTITGATYRSMAVPGWGQLYNRQYTT
                     SLLYGVGFWTFYVLFVQAAKSATTSDDLSSAALNFQLPALILWSFNVSEAATSRYMGK
                     QGLDNLRQAYRLNTQYEYVSLTERGVKFDFVLFQCSFGGGFSCHL"
     gene            complement(5256..5855)
                     /locus_tag="PLY93_00930"
     CDS             complement(5256..5855)
                     /locus_tag="PLY93_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68083.1"
                     /translation="MKILRFYILLFLAASFTANCRSSGSTIKEPRAGMPTTEFHRKPA
                     KWIPWFYANKSSRGIDRAGDVSFFNQVGAGLGVLVNSKLDDQWDGHKFFYYYLDLDFS
                     PQYTPVGPYPGTKEWTISSYPGVLFRTYLPFYLKMHFGFGVNLRYNQLFYDRWGLYGS
                     MGVELFGLTSTVVFIGHPGQPNWETEYRMGYMWVPVEWK"
     gene            complement(5852..6313)
                     /locus_tag="PLY93_00935"
     CDS             complement(5852..6313)
                     /locus_tag="PLY93_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68084.1"
                     /translation="MLLPFVQSVEAIEIGGGKFEYMNSKQTNFLGFGLFTSNKRFDAE
                     IEGFFSVQAFGDAADNVGPDYRFFFVAFSGYFHFIRTDKMSLFAGGGIMPWLPRTYAY
                     HLAVGMDFYFSENWRLFYTFRYMDNNAAEYRYPTGTSVAVGFKYAFRLLNI"
     gene            complement(6352..8061)
                     /locus_tag="PLY93_00940"
     CDS             complement(6352..8061)
                     /locus_tag="PLY93_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68085.1"
                     /translation="MVLFLALPLSVFAAKADGDSVPTLSAKNALNTPLGVRSYGMGMA
                     QTGNANDLSAIYYNPAGLAQINYVEYGLSYYSAANDVSGHSLLMSIPLPYGTLGLAGV
                     FNTVQDTEYTDKGSYSLPDRNKYSYTAALSYGMPIPRLERKLNFGTTVKWFGANFQDS
                     LPGATYPMQQKGLFIDVGLLGTFDPAQYSDALRWLPRISAGFAARNLHPLVAVDHEVQ
                     HPDNREEYNTGISLHFPYKLMLNVDAVNSPKETTRMRYGLEYWPAHFLALRGGLTKST
                     GDDLYTTIHWGIGFGETVQNSKLSFEYSGAKEFPVGVGVNFERPSSTYHRFAFHHSFE
                     VIGEERGRATPIRFTERYTHRYRFARALSPREIIADTISSLTPQEASYDSAIQAADAS
                     DVKPEIAPIEEQEEAPVTPGKPKPNQKPSYTPISLGKYVVAVYPVDVEFVAGRATSFP
                     LKEKLRGNFLLEVSRARVGRLINANKLTSAPAQRQGELESAYLKRLQQTLGADLIVFN
                     KLYVDGNYGELKLLTLYYKKGDTGLSARTEVIGSDAEETSFVAKATAQFAKSHKALLE
                     ELK"
     gene            complement(8164..8967)
                     /gene="trpA"
                     /locus_tag="PLY93_00945"
     CDS             complement(8164..8967)
                     /gene="trpA"
                     /locus_tag="PLY93_00945"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRP68086.1"
                     /translation="MHPMTRYLQERKKQKGYLFIPYIAYGDPDTKITFELICKFIEGG
                     AASVEIGLPFTDPVADGPALERAFRRSLKHEFKMSDVLAMLSKLHRKYPDFPFTVMGY
                     ANLFYQAGFKSILQKFANVGVQAVIIPDIPFDEKKYIIEEEDLDEALTRVAWIDFVTP
                     TTSAKKVNEIARLAEGFIYVVSYKGVTGQKNFSLKPLKPMIKSLRKLTRTPLLIGFGI
                     REKSQAAEASQVADGFIMGTIFHEMVEHNLERAPAIPHEIGWALPSMLP"
     gene            complement(9011..9739)
                     /locus_tag="PLY93_00950"
     CDS             complement(9011..9739)
                     /locus_tag="PLY93_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRP68087.1"
                     /translation="MPVVTHPTKQNLGRRVALGVFTIALTGASVTFLYYRFSDTPVEK
                     VEKLFLKRHLTELRDFSRKQLEKGNTNPVLYSYYAVGEFSTNPKSQLSSILSNIKAVD
                     ERPIFRREALTRILQIPSNYARAGEILTALVDFEENNDDAIKDLIARLVDGEAVLESR
                     ASNFIFLKETFPKRVKQVAAKELQWRTHPSTESDVIRRLEDGEELFVRKTEAGTIVSG
                     KKGHWAYVLDSAMQSGWVFNAYLK"
     gene            complement(9739..10377)
                     /locus_tag="PLY93_00955"
     CDS             complement(9739..10377)
                     /locus_tag="PLY93_00955"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRP68088.1"
                     /translation="MKIIKRYANRRLYDPETSRTITLDEVAQYVRDGLDIKVIDNTNG
                     EDITRKVLGQTFLKLNDSDRIANPVLQNQMLKLLIRESKDGVIEVLKKLVLAGVGMTQ
                     STSEERKTLLEMLINPSEAHEQEQRAFLKGLADKGQQEADRVLQSISGVVSSISQSIQ
                     ENMISVLDPNERARKLDGLLNQIENIQKSISKFSKSESESAKNNAVSNAKVG"
     gene            complement(10480..12828)
                     /locus_tag="PLY93_00960"
                     /pseudo
     CDS             complement(10480..12828)
                     /locus_tag="PLY93_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804124.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         3332 a         3132 c         2911 g         3453 t
ORIGIN      
        1 cacaaagtcg cacagcgcag aagcgcagaa ctaccccttc tgcactatcg atcccaacgt
       61 cggtgtggtc gccgtacccg atgcgcgttt cgacttccta aacgaaatcg tgaaacccaa
      121 aaatagcgtg cccgtcgctg tcgaatttgt cgatatcgcc ggcctcgtca aaggcgcgtc
      181 gcaaggcgaa gggctcggca ataaattctt agaccacatt cgtaaggtca acgcaatcct
      241 acacgtcgtg cgctgcttcc aagatgaaaa cgtcacgcac gtttctggca acgtgtcgcc
      301 ccgaagcgac gtggagatta tcgaactcga actcattttg gccgacgccg atcaaattga
      361 aaagcgactc cagaatcttt taaaaaagaa aaaaagtggc gacaaagaag catcggaaca
      421 aagcgcaatt ttagaacgcg ttctggaata tctcaagcag ggcaaacccg ccagcgcggt
      481 gggactcacc gccgatgaaa aatttgctat caaagatttg gcacttcttt cactcaagcc
      541 agtgcttttt atcggcaaca tcgacgaagc acttttagcc acacccgaaa aatcgcccga
      601 gttcgttgaa ctaacaaaac ttgcgcacga gcgtggtcaa gaggcaatcc ccatctctgg
      661 aaaaattgaa gcagagttgg gccaactttc ccccgacgaa gaaaaagaat tcttgaaaga
      721 tttgggtctc accgaacccg ggctcaaccg tgttatccgt gaggcgtata aactcttggg
      781 ctacatcacg ttcttcaccg caggcgaagt cgaggtgcgt gcatggaacg tcatcgccgg
      841 atcgaccgcc ccccaagcgg cgggagaaat ccacagcgac atcgaacacg ggttcatccg
      901 cgccgaggtg acgccgttcg atgcggtgaa agaatttggc tcgcaaaaaa aagcgcaaga
      961 ggcaggcaag atgcgcctcg aaggcaagga atatatcgtc gccgacggtg acgtggtgta
     1021 tttccgcttt aacgtataaa attaccataa aaaaaccgat aataaaccat gcggatttct
     1081 ttcggtgtgg tttatttttt ggtagctgcc ttattttttt ctctctttgc actaacgatt
     1141 gcagtggaaa aaaatcccct ccccgcagaa gcgttggcgt tccctgcctt tagcgagaac
     1201 tatcgtgcta aaattttaga tggcgactat gaaatttggt cagaagaaaa aatcgaacga
     1261 tggtgctcgg cgcaaggctt ggccgtcaca agcgacaacc ctcaaggggg acttcagtat
     1321 aaacgtgccg ctgtgctgat tgccccaggg cttacttttt ctttaacgcg ccccgtcacc
     1381 gaaaagggat cagagtttgc caacggctgg ctcctcaatt tggacattgc ggccatacgc
     1441 ccgcgcacgg gggtctacac caatttttta gaagcggatg tttttgtcga cggtcttttt
     1501 ttcaggaaaa ttctacaagg aggaaatcgt aaagaagaaa gtcccattca aattcaaatc
     1561 ccccacatcg ccagccgtga aggtagaatt cgggtagaga taaggcttgc aaaccaccca
     1621 cggaattttc tttttttata cgacgcgtac ctttcgcggt gattggaacg gtcttggcag
     1681 cagttatgga tagttatcct ctaatgtgac ataatccgag gaataggata gttatctcct
     1741 aatgtgacat aatccgagga ataggatagt tatcccctaa tgtgacataa tccgagaaat
     1801 aggatagtta tcccctaatg tgacataatc cgagaaatag gatagttatc cccactaccc
     1861 cattacgttt cccttagttg cccataaaat ggtagtcaat cgcaatggtc gcaaacgctt
     1921 cccatgaagt gcggtctttg tgttcataac ctgcgtaatg cgcgtctgtt gtgctatcgt
     1981 ttacaaaacc agtgcggaca aagcggtcgc tgctgtatgg gtttgtgtac caccaatgac
     2041 cttcataacc gacgctgatg ataagcccaa tttcattaaa gcggagcgca taaatgtcaa
     2101 gtgttgcgtt taagatgtac tcgttttcgt taaaacgtcc cgatttagcc atctgcgtaa
     2161 aataggttaa cgcggggaag taaaaacgcg tactcaagcg caccttagga tggaattgga
     2221 tatattgacg gtacgcaatc ataaacgttg aatacgttaa atcatcgtta cggaccgctt
     2281 ggcgcaccgg gtctgcataa tcgcggtagt accaaactcg aaggcttggt ccaactgagg
     2341 cgtcaaacca gcgaaaggca ctcccaaaat ttaagaggaa attaagcggc gttaaatggt
     2401 agtaagcgct tgcatacacc atcgtcactt cggttgcaga cgagccaacg ctctgccgta
     2461 attgttgtga aatacctgca ttggtcaagt ttgtttgttt gattgcacta aacccttttg
     2521 tgggatcgta tcccgtatcg acggggattg tcgttgtgcc ggaaggcgaa gattcggtgt
     2581 agttaaaccg agtgggcttc tcttcggtct tttgggtttt cataccgatt tcaaaatcgg
     2641 taaaaatacc tcgattcgaa cggtagtaat gaaagcctgt ttttgaatcg tggttgtctt
     2701 cgctatcttt gcctaaacga aaagctaaat cgagaccaaa gagcgcagtt atatctaaag
     2761 aatcaagcgt gtaggtgcgt gttgttttta gcgttgaatt ttcagtgcgc gtcgtaaaat
     2821 tatagagccc tgctgaaaaa ccaatggtaa acgtgcccaa cgtgggattc ttgcttatat
     2881 caagattttt gtattcccgc ttaggtaatt ttgatgctgg tgagggtgct gcaatttctt
     2941 tttcttcttt gcctgtcggc gccacttgtg tagggagtgt tttttgctgc tttgttggaa
     3001 gaggttttgt attttttttc gcagccgcac ggttcggttg ggttcccttg cgtggggcag
     3061 cactgacgct tccccaagct gtcattaaaa agatagctaa tattattatg cccgctgcgc
     3121 ctttcattgt accgccacaa agttggaata gagacggcct gtcttgccat cgtatttgta
     3181 gtcaaaacga attatataat aacctttgta agagccggtt accatttgag tgctttcttt
     3241 tgcattaggg atcgtatgta ccggtgtgta tccccctaaa gcacctgctg ctttcaccgt
     3301 ggtaatgtcc gctgccgagg gagttgtatt cttaaaaagg acttgagctg tgccgctcac
     3361 tttaatctcg tcttcggtga gctctgaaac actgccttta gcgtctttat aaaagatgtg
     3421 cccccaagat aatttagcgg aagtacctgt atcttcaccc acaagactta aacggaagga
     3481 accgctatcg taaatggggt ctaatggatt tgagtgctct tcggcaacct cggtaaaggc
     3541 acaagacaat aaaaaggtaa gagcaataaa aagaacgagt gctgatttta attttaccat
     3601 tttggcctcg tattcttcat catgaactct tggaaaaaag aaagctgcgg tgtataattg
     3661 agcttgcccg caaaatactt ttggtgcact tggtacagca catcgtcggc gcgctcgcgg
     3721 tcttcaaacg catcccacac cgagtacgcc catgtcagcg caccaatcac atacatggtg
     3781 cgcgccacat cgcgattgtt tcgcatcgct ttatactttt tttctgcatc cccgggagtc
     3841 aatgcagcct cgtaatcttt ttgagcctgt gcaattttgg tttcgcgtga ataaccatag
     3901 ccaatcgaac catacgtgat gagtgccatc gcagtaccgg tgacatagtt cttgttatag
     3961 aactgccccc aacccgaaag cacaattgaa cggatacacg ctccctcggc agttttgata
     4021 atctcagtgg tgaaataatc caaatcaaaa aaactcactg tcacttggca atccatcacc
     4081 gcgacataga atggtgagaa gtcaataccg tgttcccatt tgggatcgct acaacgcgct
     4141 tcgtcgatta ctttagcatt tacatcggta atgagctgct cgtcggcgat ttgcatatct
     4201 ttcgaggtcg tcagcatgtg gcggaagctc gaaatcgaac gcgccaatgc tttattgtgc
     4261 gcatcaattt tagcattata gatggcaatt tcttgcgaac gacctttacc gatggcatcg
     4321 cgcacacgca caactttttg cgttgagaaa tattctttat caagccccaa cgtctctccg
     4381 cttttaccgc ccgctgcgtc cacgaagctg gggataaaga caaaaaataa tagcaaggcg
     4441 gtgtttttta tagatggcat gaaaaacctc caccaaagct gcattgaaac aacacaaagt
     4501 caaatttcac gccacgctcc gtcaacgaaa catattcgta ttgtgtattt aagcggtacg
     4561 cttggcgtaa attatccagc ccctgcttac ccatatagcg cgatgtcgct gcttcagaga
     4621 cgttaaagct ccaaagaatg agcgcgggta actgaaaatt gagtgctgcc gagctcaaat
     4681 catcgctcgt cgttgccgac tttgcagcct gcacaaaaag cacatagaaa gtccaaaagc
     4741 ccaccccgta aagaagactg gtggtgtatt gccgattgta aagctgaccc catccgggca
     4801 ctgccatcga gcggtacgtc gctccggtga tcgttttttc ataatcttcc atgaatttag
     4861 gaagatcgac gtagtccagt tgcatcgtcg cgctcaaatt aacgcgcaac ggttcacctg
     4921 cggggcgtga aaatcgataa ttcgtgattt gcacatcggt gacttgaagg tgtgcctctt
     4981 gcttcaccca tgtgtcttgt aaaacgaagt ctttactttc gctacgtccc acgagctccg
     5041 attcacccag acttgtaccc actttcatcg cctgatcgac agcattatta aaagcttcga
     5101 cgggatcatt atttaagctt tcacccacgc cgtcttcagt actaattttc accttgaaac
     5161 tgttggtata ttcgcccgct acaagaggga aaacagccac taacgcaata agaagaataa
     5221 aatgttgtgt gtatttcata acttggttct gcatattatt tccactccac aggaacccac
     5281 atatacccca tccggtattc ggtttcccaa tttggctgcc ctgggtgacc aatgaacacc
     5341 acggtcgatg ttaatccaaa gagttcaaca cccatggaac cataaagacc ccagcggtca
     5401 taaaaaagtt gattatagcg taaattaacg ccgaagccaa agtgcatttt tagataaaat
     5461 ggtaaataag tgcggaacaa caccccaggg taacttgaaa ttgtccattc ttttgtgccc
     5521 ggatagggac ccaccggagt gtactgaggt gaaaaatcta aatctaaata ataataaaaa
     5581 aacttatgac catcccactg gtcgtcgagt tttgaattca ccaatacacc cagaccagcc
     5641 cctacttgat taaaaaaact aacatccccg gcacggtcga taccccgtga cgatttgttt
     5701 gcatagaacc aaggaatcca ctttgccggc ttacggtgga attccgtggt gggcattccc
     5761 gcacgtggtt ctttaattgt cgaaccgctc gaacggcaat ttgccgtgaa agaagcagct
     5821 aaaaagagga gtatgtaaaa gcgtagtatt ttcatatatt taacaaccgg aatgcatact
     5881 tgaatccaac agcgaccgag gtgccggtgg gataacgata ctctgcggca ttgttatcca
     5941 tataccgaaa cgtataaaac agacgccaat tctcgctaaa ataaaaatcc ataccaacgg
     6001 caagatggta cgcatacgtg cgcggcaacc aaggcataat accaccgcca gcaaaaagac
     6061 tcattttatc ggtgcggatg aaatgaaagt aacctgagaa agcaacaaaa aagaagcggt
     6121 aatcagggcc aacattatct gcggcgtcac caaacgcttg cacagaaaag aatccttcga
     6181 tttcagcatc aaaccgtttg ttcgaggtga aaagaccaaa gcctaaaaaa ttcgtctgct
     6241 tcgagttcat gtattcgaac ttgccgccac caatttcaat cgcttccacc gattgaacaa
     6301 aaggtaggag caaaaacgag aacaaacaga gctttaacca aggacgcagc attatttgag
     6361 ttcctccaaa agagccttgt gcgatttggc aaattgtgca gttgctttag cgacaaacga
     6421 agtttcttca gcatcgctgc cgataacctc agtgcgcgca gaaaggcctg tgtccccttt
     6481 tttgtaataa agtgtgagta acttcagttc cccgtaattg ccgtcgacgt agagtttatt
     6541 aaagacaatt aaatcggcgc ccaacgtttg ctgcaaccgt ttaaggtacg cactctccaa
     6601 ttcaccttgg cgttgagcgg gcgcagacgt taacttattc gcattgataa gacgtcccac
     6661 gcgcgctcgg ctgacttcca acaagaagtt accgcgtagc ttttctttta gaggaaacga
     6721 tgttgcgcgg ccagcgacaa actcaacgtc aacagggtac acagcgacga catacttgcc
     6781 caatgaaata ggcgtgtacg atggtttttg gtttggtttg ggctttcctg gagtaacagg
     6841 agcctcttct tgttcttcga tgggcgcaat ctcaggtttg acgtcgcttg catcggccgc
     6901 ttggatcgct gagtcgtaac ttgcctcttg cggagtgagg ctgcttattg tatcggcaat
     6961 aatttcacgc ggtgacaagg cacgggcaaa gcggtatcgg tgtgtgtaac gctcggtaaa
     7021 gcggatgggg gtcgcacggc ctcgttcttc accgatgact tcaaacgagt ggtggaatgc
     7081 aaaccggtgg taagtcgagc taggacgctc aaaattaacc cctaccccca ccggaaattc
     7141 ttttgcgccc gagtactcaa aagataattt actgttttgc acggtctcgc caaaaccaat
     7201 cccccagtgg attgtcgtgt acaagtcgtc accggttgat tttgtcaatc caccgcgcag
     7261 cgctaaaaag tgcgctggcc agtactctag accatagcgc atacgtgttg tttctttggg
     7321 gctgtttacc gcgtcgacgt tgagcatcag cttataagga aaatgcaaac taattcctgt
     7381 gttatactct tcacggttat cagggtgctg cacttcatga tcgactgcca ccaaggggtg
     7441 taaattgcgt gcggcaaaac ccgccgaaat gcgcggcaac caccgcagcg catcgctata
     7501 ttgcgcaggg tcgaatgtgc ctaaaagccc aacgtcaata aaaagacctt tttgctgcat
     7561 ggggtacgtt gcgcccggca atgaatcttg aaaattggct ccaaaccatt ttacggtagt
     7621 tccgaaattg agcttacgct caaggcgtgg aattggcata ccatacgaca gcgccgcagt
     7681 gtacgagtat ttattgcgat cggggagaga atacgacccc ttgtcggtat attctgtgtc
     7741 ctggacagtg ttaaaaacac cagcaaggcc aagtgtccca tacggaagag gaatagacat
     7801 gagcaacgag tgccctgaaa catcgttggc ggcgctataa tacgacaatc catattcaac
     7861 gtagttaatc tgcgctaagc ctgccgggtt gtagtagatg gcggataaat cgttggcgtt
     7921 accggtttgt gccatgccca taccatacga gcgcacccct agtggagtgt tcagtgcatt
     7981 ttttgctgaa agtgttggta cggaatcgcc atcggcttta gcggcaaaca ccgataaagg
     8041 cagagcgaga aacaatacca aaattaaaaa tatattccgt gtcgttttca cagggtgtaa
     8101 ttatccagtt ggccatctct ggcatttttt ttcttttgtc aacaaaaagt aaagtttttg
     8161 attctatggt aacatgcttg gcaatgccca gccaatttca tgcggtattg ccggtgcacg
     8221 ctcaagattg tgctcgacca tttcatggaa aatggtaccc atgatgaaac catcggcgac
     8281 ttgcgacgcc tctgccgctt ggcttttttc ccgaatacca aacccaataa gaagcggtgt
     8341 gcgggtgagt ttacgcaacg actttatcat cggtttgaga ggcttaagcg aaaagttttt
     8401 ttgccctgtg actcctttat aagagacaac gtagataaaa ccttccgcca agcgcgcaat
     8461 ttcgttcact tttttagcgc tcgttgtcgg cgtcacaaag tcaatccagg caacgcgcgt
     8521 gagcgcttcg tcgagatctt cttcttcaat gatgtatttt ttttcatcga aaggaatatc
     8581 tgggataatg actgcttgca ctcccacatt ggcaaatttt tgtaaaatcg acttaaatcc
     8641 cgcttggtaa aaaagatttg cgtaccccat aacagtaaaa gggaaatcgg gatatttgcg
     8701 gtgcaactta ctgagcatcg cgagcacatc gctcattttg aactcgtgct tgagagaacg
     8761 gcggaacgca cgctcgagcg cagggccatc ggctacggga tcggtaaacg gtagaccgat
     8821 ttcaacagac gccgcgccgc cttcgatgaa cttgcaaatg agttcaaacg tgatcttggt
     8881 gtccgggtcg ccatacgcta tgtaggggat aaaaaggtaa cccttctgtt tttttctttc
     8941 ttgcaaatac cgcgtcatcg gatgcatact ggtttataca tcggcaaaga aaaagcacgg
     9001 caagcaaaat ttatttcaaa tacgcgttaa aaacccaacc cgactgcatc gcgctatcga
     9061 gcacatacgc ccaatggcct tttttgcctg aaactatcgt acccgcttcg gttttgcgta
     9121 caaacaactc ttccccatct tctaagcggc ggattacgtc actctcggtg gagggatgcg
     9181 tgcgccattg gagttctttt gccgcaacct gcttcacccg cttggggaat gtttctttaa
     9241 ggaaaataaa atttgatgcc ctactctcca ataccgcttc tccatcgaca aggcgggcga
     9301 taaggtcttt tattgcgtcg tcgttatttt cctcaaaatc gacgagtgcg gttaaaattt
     9361 cgccggcgcg ggcgtagttt gagggtatct gtaaaatacg cgtcagcgct tcccgccgaa
     9421 aaatggggcg ttcgtccacc gctttaatgt tgctcaaaat actggataac tgcgacttag
     9481 ggttcgtgct aaactcaccc acagcgtaat aggaataaag cacggggtta gtattccctt
     9541 tttcaagctg cttacggctg aaatcgcgca attcggttaa atgccgttta agaaaaagtt
     9601 tctccacttt ttcaacggga gtatcgctaa agcggtaata taaaaaagtt acactcgccc
     9661 ccgttaaagc gatggtaaaa acacccaatg cgacacgtct tcccaaattt tgtttcgttg
     9721 ggtgtgtaac gacgggcatc agccgacttt ggcgttactt acagcattat ttttagcgct
     9781 ctcgctttca gacttactaa acttactaat cgacttttga atgttttcga tttggtttag
     9841 caatccatcg agctttcgtg cacgttcgtt ggggtcgagc actgaaatca tattctcttg
     9901 gatcgattgc gagatgctcg acaccacacc tgaaatactt tgtaaaacgc ggtcggcctc
     9961 ttgctgtcct ttatccgcca accctttcaa gaaagctcgc tgctcttgtt cgtgcgcttc
    10021 ggaaggattg atgagcatct cgagtagagt tttgcgttcc tcggatgtac tctgcgtcat
    10081 acctacgccc gctaaaacaa gtttttttag cacttcgata acgccgtcct tgctttcgcg
    10141 gattaaaagt ttgagcattt gattttggag cacgggattt gcaattctat cgctgtcgtt
    10201 taatttaaga aaggtttgcc ccaaaacttt acgggtaata tcttcgccat tggtgttgtc
    10261 gatcacttta atatcgagac catcgcgtac atactgcgcc acttcgtcga gagtaatcgt
    10321 acgcgatgtt tcggggtcgt agagacggcg gtttgcatag cgtttaataa tcttcataat
    10381 gttattcctt ctattttctg acgcgggtca cagaacccac tcgactttat agtgcattgc
    10441 acgaaacaaa gacactatgt caatgaagat agaaaactcc taaccaaaca ccacttgctt
    10501 gcccttacag tgcgcagtga ttttcttccc tcgctcaaaa ttaccctcaa gcagggtttg
    10561 tgccagaggg ttcaacagca agttttcaat cgctcgtttc atagggcgtg cgccgtactc
    10621 ttcatcaaaa ccttgctcga cgatgagttt ttttacgtcg tcacccaccg ttaagtcgat
    10681 gccacgcgcg cgtatttttg cgaccacctg atcgacctgc aagccggcaa ttttttctaa
    10741 gtggccttgg ttcagcctgt ggaactgaat gactgcatcg agacgattca agaattcagg
    10801 acggatgcac gcactgtgtt taacacacgc acctcttgct cctcgctgtt taagtcttta
    10861 ttcgctaagt gttccgaacc caagttagag gtcataataa gaatcgtatt cctaaaattc
    10921 accaatcgac cttttgcatc ggtgagtctt ccctcgtcta aaatttgcaa gaaaaggtta
    10981 aacacatcgg ggtgcgcttt ttcgatttca tcgaacaaga tgacgctata cggacggcgg
    11041 cgcacttttt ctgtgagctg gccgccttcg tcgtaaccaa tgtaacccgg cggtgaaccg
    11101 atgagcttgg ccaccgagtg tttttccata tactcgctca tatcgatacg caccatcgct
    11161 ttggcgtcgt cgaaaagata ttcggcgagc gccttggctg tttcggtttt gccaacgccg
    11221 gtggggccta agaataaaaa gctgccgatg ggtttatctt cgtcggctac acccgcacga
    11281 ttacgacgga tggcgtgcgc aattgcatcg acagcgtctt cttgccccac aacccgctgg
    11341 tgtaaaacgc tttcaatttt caagagcttc gctttttcac tcgtgagcat tttcgacaca
    11401 ggaatacccg tccaacgcga aacaatttcg gcaatgtctt cttcggtgac ttcctctttt
    11461 aagaggcgca tttcagaatt tttctttttc atgttttcga ctgtcacctc cagtttcttt
    11521 tggagtgtcg gtatttcgcc gtagcgcaac tcagcgacac ggtttaaatc ccccttgcgc
    11581 tctgccgcac tttctgcaat tttcaacttt tcgatttctt ctttaataat agacacttgc
    11641 gacaaatctt ctctttcgcg gtcaagctgt agcttcattg tgttgaaatt ttcacgtaac
    11701 tccgaaagct ctttgcgcac cgccgctaag cgttccactg aagcagcatc gttctctctg
    11761 ttgagcgcag tctcttcgat tgtaaggcca ataatttttc ggtttaagtc atccaactcc
    11821 tgcggcatcg agttgcgctc aattttaagt tttgccgccg cttcgtcgat gagatcaatc
    11881 gccttgtcgg gcaaaaagcg gtcgctgata taacggttcg agagcgtcgc cgccgacaca
    11941 atcgccgcat cagtgatgcg tatgccgtgg tgtacttcgt agcgttcgcg caatccacgt
    12001 aaaattgtga tggcatcttc caccgaaggc tcgttcacat acacagtttg aaaacgacgt
    12061 tcgagcgcgg cgtctttttc taaatgcttt tgatattctt taagggtcgt tgcgccgatg
    12121 aggcggattt ctccccgcgc cagcgctggc ttgagcatat tggccgcatc gagctgtcct
    12181 tcagtggcgc ctgcgcctac aacggtgtga atttcatcga tgaacaaaat aagtttatct
    12241 tggttcgctt tgacttcatc taaaacagcc ttgagtcgct cttcaaattc accacggtat
    12301 tttgcgccag cgataagaga acccatgtcg agtgtcacca cagttttatc gcgcaaaatt
    12361 tcgggtacat cccccgaaac aatccggccc gccaagcctt ccacaatcgc tgttttacca
    12421 gtacccggtt cgccaatcaa aagcgggtta tttttattcc ggcgtgaaag aatctgcacc
    12481 gtacggcgga tttcttcgtc acgcccaatc accggatcga gtttcccttg ccgcgctaag
    12541 tcgttcaaat tcttgccata tttttgaagc gcattgaatt tcgcttcggg attatccgaa
    12601 tcggcttttt ggcttccacg caattcttta agcagtggcg ccaacgaacg gcggtcaagc
    12661 cccaaactct ctaattcttt tttgagtgca aagttaccgt ttgcatacgc aagcagcaag
    12721 tgttcaagag agagataatc atcacccaaa ccgtcttttt cccgctcagc aagttgtaaa
    12781 aatttacgcg catcacccga aagattttgc cctgcgtcaa aatctccc
//
LOCUS       DAOMPD010000012        12671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2136_length_12671_cov_2.718136, whole genome shotgun sequence.
ACCESSION   DAOMPD010000012 DAOMPD010000000
VERSION     DAOMPD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12671
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2136_length_12671_cov_2.718136"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..591)
                     /locus_tag="PLY93_00965"
     CDS             complement(199..591)
                     /locus_tag="PLY93_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68089.1"
                     /translation="MFTDKATDAFRVGAAVSLPRAGGGSVVGTMIDVNGDGVPDGIDL
                     DGDGVPEILFKNLVAGESAGLDLNGDGAIDYYLSVDFQGNFYLSTAKPVGNATSGNVA
                     VTTDTNGTPTGFNTTGSTTVDDAVLGQI"
     gene            complement(678..1802)
                     /locus_tag="PLY93_00970"
     CDS             complement(678..1802)
                     /locus_tag="PLY93_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68090.1"
                     /translation="MSAAETIWANDTPTDLVPRRVLVLDFVNQANDKKSDYLSVSVAE
                     ALLDPLKQTGKFYLLPRDASVGPIPPKRRPEDGSRYDSPNGESLGTSSELRAGSTQKA
                     SITFDETEAVQIGKAADADVVVIGNFVSIGNRVQIQTKAVDVQTGQIAVAKTVSGKLD
                     ATIFDTIQKLATTMSQEMAVKLPPLPRREIIYGVGTSAYSATGDFIVRVPVVHGYLSG
                     SFVWGRESKYVLFGYGAAADVSFQFLHRLIQPYFAANASFASGVTRVTTMNFFGFDAG
                     LGYGITAPRYSPAGHSGQAPFLQGIQIRPVIGGGFQTGTIKTSYNIDYFVPSVFVGVV
                     GDFFIHEKFSVALALKQYILIEEDTSLKIFTVQVGVGVRL"
     gene            complement(2110..3417)
                     /locus_tag="PLY93_00975"
     CDS             complement(2110..3417)
                     /locus_tag="PLY93_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRP68091.1"
                     /translation="MRVKTERWPVPHEDAPYQKEVSALLHALLSQSTLEGLGGFILDA
                     AHEYLAADTALFFQVDSEEKFYTLNLSRGFPNPTHRLQRFNIRSGVAGRAITTGKTYY
                     TPNTVNDKHYAARQENMHSALAVPIRCGGETLAVVEFFSSRGAAFDRDRIQFVELLFA
                     YAAPQFEKLYALQRTQRRAALSNTLAETLSLFVQTKNAESVLQEMLRILAQSRLFQEA
                     AFFFYRAETKQLERIAAWPRKEAEAPHLSLKINESAAGYVFKTQQAYFLRDALHKKSP
                     LSAQEKHADALTPTGFIAVPIFIAGTVQAVLSLHTPFVSQALFTEYIAFAEGLAQNLS
                     FVLQNHWAYFEKTYGGQDNTTTDTDNTADGVLKSVRAFLKSTMAESPTGKIFENTVSA
                     IEKEVIRIELSQHQFNKSKTAEALGLNRVTLDKKIQQHKLFEV"
     gene            complement(3427..5937)
                     /locus_tag="PLY93_00980"
     CDS             complement(3427..5937)
                     /locus_tag="PLY93_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68092.1"
                     /translation="MKVSTLILLFFMGAVQLSARSWGDLDQNESRAYGREREEKYPVR
                     LFFVEREAWENHHAWHALFLWGQKNYPRYESTWMFPFFKSLESKIDKRSYFLTPLSYK
                     EEDITSWDFSLFWLYYSGEEKSPHSRYHALAPFYYYSTDEKKSYSILTPLFWYHRQLD
                     KEYSSLVTPVSFTLSRAGESDTSLLWIVYWGKSDTEKYFYHAIFPLYFALHETQGLQF
                     YSPLYWKSDTADSSFQMLLPIYLSYRREGYALHIDALGFSFAEENASALPVGVSVNDK
                     TLLVDTDFGWFYNLLRLSWRESIHLDADEKDTATTNAALPQTPALSQTRMRDRAHSED
                     FRGVYLLFGLFAYEHADTYRHIRLLPLSWLTWDKNSDTGVKALLPFYFNYKDESVEYK
                     IIAALLPLYGKQQQFFPECTSSTEAWLAIVYWSEYDCKKNWSEKTVLWPFVNTYNAPN
                     EGGYRIFPLFWYKWWVENNKRAYRHFSPLHITTGTENFTDTYTWLFYRARSEQKSTWG
                     VWALLHFSHNANQTLMEDTTYVLPFYYTTTTYLPSESNPRGSSSSLFSFAALFWRYAE
                     IRDAREDFTLHISPIYFYAQENQRSYFFSWVYYDFQKNTSRSLGIPLLFHRYTEESNE
                     YKNFYIFPLYQSWQQSAAGEIYTTWFFPLFYYEGAPQSVDFQAAFGLLGGFKKSTQVL
                     SWHAALLVGYKYVKDAYTDHYVLPLWYHSRYRTELGMSSETYIPILLSKFSSDNNGAR
                     VKRILFLGLLYYYNSERENFYQREHLLLGTLYYHNKKYERATDGHPRHFESYGSLWGL
                     LWHYETEENYKRFSFLTFVYARTERDGNVRHRVMGFSF"
     gene            6059..>6853
                     /locus_tag="PLY93_00985"
     CDS             6059..>6853
                     /locus_tag="PLY93_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRP68093.1"
                     /translation="MSDKKVLNWRAAIHTPKLFFFCAVIFFSRCKNFGHFWDSPPEHK
                     ANTSASVTYWRTADTGQTQCYDNTTTATCTGNAGSYPNQDADFVDTPRARSFTGPTQH
                     SIYWADYTTVDNVTGLIWKTCTEGKTGATCTGTAGNYTISPDTATPACTALNTANSGV
                     GYAGRTDWRLPTVSELQTLALYSGASIAIDATAFPGAVASSYWSSTSDVYTPANSWFV
                     SYIDGAVSSTAKSTAYQVRCVSPGPLRAAAYTDNGDGTVSDASTGLV"
     assembly_gap    6854..6863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6864..7208
                     /locus_tag="PLY93_00990"
     CDS             <6864..7208
                     /locus_tag="PLY93_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRP68094.1"
                     /translation="QQCSAGLSGNSCTGTLTPLVWSAALTYCKNLTLAGRSWRMPSAN
                     EIMTLADYTATNPAINLTYFPGTPAAENYWTSTTLPGTPANARHFFIQLGILHNMVKS
                     AAIQIRCVANDL"
     gene            complement(7290..7895)
                     /locus_tag="PLY93_00995"
     CDS             complement(7290..7895)
                     /locus_tag="PLY93_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRP68095.1"
                     /translation="MKLYYASGACSMATHILLEWIGKPYELQKVGLHPKSPELVAANP
                     FGAVPVLEDNGFTITQNNAILHYLTEKFPEAKLMGDGSLKVKAKINQYLGIINADLHP
                     LFKPFFGSTAYLEDAAAIEKSKKNAATQVRTKLELIDRSMSGVYLAGVRSIVDPYFFV
                     MLNWCNSMQIQFDDLKNVSMFFESMKKDPAVFKVLKDEGLI"
     gene            8000..8608
                     /locus_tag="PLY93_01000"
     CDS             8000..8608
                     /locus_tag="PLY93_01000"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP68096.1"
                     /translation="MAYRRMIGASASEDRLEKLIEDRLKEGADKEKIDSRIWDLFGES
                     WAVMFTDLSGFSRNVAKFGIIHFLQTIYESERLLIPIIEDNDGVLLKAEGDSLLVIFR
                     NVTKALHASIFMQRTLLAYNKDKSDEEKVLLCVGLGYGKVLRIGDVDVFGHEVNSASK
                     LGEDTAEAYEVLVTPAVAENAATQKEFNFEKTEAGHFKLLWR"
     gene            complement(8628..10727)
                     /locus_tag="PLY93_01005"
     CDS             complement(8628..10727)
                     /locus_tag="PLY93_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68097.1"
                     /translation="MRIFFVHITLALFCALAAQAKEVTFYLTSNLAGRFPIGANEKDA
                     DSTIIRIAGALRAAKESYPNAYHFDLGNAFYPGRLSRFSFGSLTADYFQMVRLDAGIV
                     AAADLNIGAESLEYIRRARGIRLVSANIFRDKTPLFEPYTVIKKGEVKAAIVGLTSAR
                     SLVAYEEAEALELRLALPKDVLTETIERVSTESPDIVVVLTSVSVSDAIQLLAKHRRI
                     DIMICGGDAESALGQSPIRTIELPDGRRLIALPPEASLTKVHLKQTRSGWQLTEQENV
                     GLLDIPKSAEPPPSFTRRLRLWQKWYTTDANGVRDASFSPVTLTPKFAADAVREESNC
                     DVSFIEASDIAVTDNSTLTDANQIRYNVENDYNIFVFGMTGEALRKFYTKYPGLVYSG
                     FDKNKITGYPIREKVKYRVCATQRGYELAERENGESLGGKTQWFGISDAVVNFAKNNE
                     ERTPAQTADSYFRLMTTFNLSNIYESGKISNPGAIETPPGQPTDSYIKWGLENDVNFL
                     FYNRRHAFSFNPYIFYMRQSNDIIRNLLRGEVTYTYNTEWYINPYQRNRIDTVVVKDP
                     TTGLRPSLLRETAGGEFSYGIFTGRLGVGLEKQILDPVNVAFGGIEATLKIQWEFIRG
                     VTYKLAFDSFSATSTNGEWRNRLELNNAMIFTIASPLTFTVSHRYYYFYLGYIQNFYD
                     ASVFTFSFDLRSTWKFP"
     gene            complement(10727..11293)
                     /locus_tag="PLY93_01010"
     CDS             complement(10727..11293)
                     /locus_tag="PLY93_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68098.1"
                     /translation="MRYQFLRLMHALSIFLFVFFLASCASWQDRSSDQIAAERALIQR
                     KTALLERENQILRDENLELTRAVEVAKAEAEKKAASFVAEQEKQAASMKALETHLTNL
                     TDKIAILESDSVGKIRKLTQLNEQLTQKSIEAQKKLQDELADAQQKAAKEKEKLQKEA
                     ADKQFAQEKELELLRRELSELKSKTAPH"
     gene            complement(11302..12318)
                     /gene="purM"
                     /locus_tag="PLY93_01015"
     CDS             complement(11302..12318)
                     /gene="purM"
                     /locus_tag="PLY93_01015"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRP68099.1"
                     /translation="MDYKSSGVDTDYAQQLIHKLKDKISATHRVPRVGTVVGKYGGFA
                     GRFEAVQIPGFDVVAATDGVGTKTQLLRRFNYLEGIGQDLVAMCVNDLFCSGATPAFF
                     LDYIACGHLDEKWYNRVLSSIVDACAQSPMALMGGETAEHPGVMPDDDFDLAGFAVGF
                     VPRSRVLPNFETQKAGDILVGVPSSGLHSNGFSLVRKILNEIEKSDPIGFQTMVANRA
                     ALIETWLQPTRIYHEIPRLLEKVPIKGIAHITGGGIHENLPRIIASGLGAVIENPEPF
                     RLKAIETLEKFVPREKLFHTFNMGVGIILIAEESELMAIREIFPKATVMGKLSRIEGV
                     AIHF"
     gene            complement(12382..12671)
                     /locus_tag="PLY93_01020"
                     /pseudo
     CDS             complement(12382..12671)
                     /locus_tag="PLY93_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071670.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
BASE COUNT         3249 a         3035 c         3086 g         3291 t
ORIGIN      
        1 gctaatcgtg accgtcgccg tattgcccgc caccacgacg ccgtttgcag gcgggccaaa
       61 gctgggtgtc gtgccatcgg tggtgtagac aatcgcattg caggcgacat cgtctgagca
      121 agtgagtgtc acggtttgcg cctcggtgaa tgtaccgcca gcgttgttac ccgccgtcgt
      181 aggcggcgtg gcgtcggtct aaatttgacc cagaacagca tcatccaccg tcgtactgcc
      241 ggtggtatta aaacccgtcg gcgtgccgtt tgtgtcagtc gtcaccgcaa cattgcccga
      301 cgtagcatta cccacaggct tcgcagtgct caaataaaaa ttaccctgaa agtcgacgct
      361 gaggtaatag tcgatggccc catcgccatt gaggtcaagc cccgccgact cacccgccac
      421 aagattctta aacaaaatct ccggcacccc gtcgccgtcg aggtcgatac cgtcgggcac
      481 cccgtcgccg ttcacgtcaa tcatcgtgcc aacaacgcta cccccgcccg cacgcggcaa
      541 cgacacagcc gcccccacac gaaacgcatc agtcgccttg tcggtaaaca caccgccgaa
      601 cccgcggcag gataataata ataatatcgt agggacgcag catgctgcgt ctctacgtat
      661 tctcataaat ttattcttca caacctaacc cccacaccca cctgaacggt gaaaattttt
      721 aatgacgtat cttcttctat taggatgtat tgcttcaacg ccagcgcgac gctgaatttt
      781 tcgtggataa aaaaatcacc caccacaccc acaaaaaccg acggcacaaa gtaatcgatg
      841 ttgtacgaag ttttaatcgt acctgtctga aaccctccgc cgataacagg gcggatctga
      901 ataccttgta agaaaggagc ctgccccgag tggcccgcag ggctgtatcg aggggcagta
      961 atcccatacc ccaaccccgc atcgaaacca aaaaagttca tcgtggtaac gcgcgtaacg
     1021 cccgaggcaa aagacgcatt cgctgcaaag taaggttgaa tcagccggtg cagaaattga
     1081 aaactcacat ccgccgctgc gccatagccg aacaatacat acttgctctc acgcccccac
     1141 acaaacgagc ctgaaagata accatgcaca accggcacgc gcacaataaa gtcaccggtt
     1201 gccgagtatg ccgaagtacc gacaccataa ataatttcac gccgaggcaa cggtggcaac
     1261 ttcaccgcca tctcttgcga catagtggtg gcgagttttt gaatcgtatc aaaaatcgtc
     1321 gcatccaact tgcccgacac agtcttagca acagcaattt ggccggtttg tacgtcgact
     1381 gccttggttt gaatttgcac gcggttgccg attgaaacga aattaccaat caccaccaca
     1441 tcagcgtcag cagccttgcc aatttgtacc gcctctgttt cgtcgaaggt aatggatgct
     1501 ttctgtgtgg agcctgccct aagttccgag cttgtcccga gtgattcgcc gttaggcgaa
     1561 tcgtaccgcg acccgtcttc gggtctccgc ttcggcggga taggccccac tgaagcatcg
     1621 cgcggcagta aataaaactt ccccgtctgt ttcaacggat ccaagagtgc ctctgcgacg
     1681 ctgaccgata ggtaatctga tttcttatcg ttcgcttggt tgacgaaatc gagcaccaac
     1741 acacggcggg gcaccaggtc ggtgggggta tcgtttgccc atatcgtttc ggctgcgctc
     1801 aacgatatga ttacagtgaa tgctattcgt agagccgcga tgaatcgcgg ctctacgaag
     1861 aaccatttat gtttgccgaa atgtctattt aatgacgaga gccgatttgt tgcgtgttgc
     1921 gtgttgcgtg ttgcgtgttg cgtgttgcga gcgccgagtg ccgagtgccg aaggcgtatc
     1981 gaggggtgcg ctgtgtgatc gtatcgttga gcgcagccga aacgatacaa gctgtctgtc
     2041 tatctatcac ggtagccttt atagaatttt cgatgaaaat gtcaaagata tttgtatgga
     2101 atctccccct tacacttcaa acaacttgtg ctgctgaatt tttttatcca gcgtgacgcg
     2161 gtttaagccc aacgcttcgg cggttttgct tttgttgaat tggtgttggc tcagttcaat
     2221 gcggatgact tctttttcta tggcactcac tgtgttttca aaaattttac ccgtgggtga
     2281 ttcggccatt gtgcttttga gaaaagcacg taccgattta agcacaccgt ctgcggtgtt
     2341 atcggtgtcg gtggtggtgt tgtcttggcc gccgtatgtt ttctcaaaat acgcccagtg
     2401 gttttgtaac acgaacgata gattttgagc aagcccttct gcaaatgcaa tatactcggt
     2461 gaacaaggct tggctcacaa agggagtgtg tagcgaaaga acggcttgca ctgtgcctgc
     2521 tatgaaaata ggtacggcaa taaagccggt cggtgtgagc gcatcggcgt gcttttcttg
     2581 cgcggataat ggcgacttct tatgtaaagc gtcgcgcaaa aaatatgctt gctgcgtttt
     2641 gaaaacgtag ccggctgcgc tttcgtttat ttttagtgaa aggtgtggtg cctcggcctc
     2701 tttgcgcggc catgcggcaa tgcgttcgag ctgttttgtt tcggcgcggt agaaaaaaaa
     2761 tgcggcttct tgaaagagtc ggctttgcgc cagtatgcgt agcatttctt gcaatacgct
     2821 ttcggcgttt tttgtctgca cgaagagtga taatgtttcg gcgagcgtgt tagaaagcgc
     2881 ggcgcggcgt tgggttcttt gtagcgcgta gagtttttca aactgcggcg cggcgtacgc
     2941 aaacaagagt tcgacaaatt gtattcggtc gcggtcaaac gccgcaccgc gactggagaa
     3001 aaattctaca accgcaagag tttcgccacc gcaccgaatc ggcactgcca acgccgagtg
     3061 catgttctct tgccgcgcag cgtagtgttt gtcattcacg gtattggggg tgtaatacgt
     3121 tttaccggtg gtgatagccc tacccgctac gccgctgcgg atattaaacc gctgtaatcg
     3181 atgcgtcggg ttaggaaatc cccgcgagag gttcaaggta taaaattttt cttcactatc
     3241 gacttgaaag aagagcgcgg tatcggcggc aaggtattcg tgcgcggcgt ctaaaataaa
     3301 accgccgagg ccttcgagcg tcgattgcga taaaagcgcg tgcagaagcg cagacacttc
     3361 tttttggtaa ggtgcatctt cgtgcggcac aggccagcgt tcagttttta cacgcacggc
     3421 aaagtatcaa aacgaaaaac ccataacacg gtgccgcacg ttgccgtcgc gttcggtgcg
     3481 tgcgtacaca aacgtcaaga aagaaaaccg tttgtaattt tcttcggttt catagtgcca
     3541 taagagcccc caaaggctgc cgtacgactc gaagtggcgg ggatgaccat ccgtagcacg
     3601 ttcgtatttt ttgttgtgat aataaagcgt acccaaaagc aagtgctcgc gttgataaaa
     3661 attttcccgt tcgctgttgt aataataaag aagccccaaa aaaagaatgc gctttacccg
     3721 tgcaccgttg ttgtcgcttg aaaattttga aagaagaatg gggatgtatg tctcggatga
     3781 catgccaagc tccgtgcgat atcttgaatg ataccacagc ggtaacacat agtggtcggt
     3841 gtacgcatcc ttcacatact tgtagccgac gaggagcgca gcgtgccaac tcaacacctg
     3901 tgtgcttttt ttgaaaccgc ctaagagacc gaacgcggct tgaaaatcca cgctctgcgg
     3961 agcgccttcg taataaaaaa gcggaaaaaa ccacgtcgtg tatatttcac cggcggcact
     4021 ctgctgccac gattggtaga gcggaaaaat ataaaaattt ttatactcgt tactttcttc
     4081 ggtataacga tgaaacagaa gtgggatgcc caaactgcgc gatgtatttt tttgaaagtc
     4141 atagtacacc cacgaaaaga aataactgcg ctggttttcc tgagcgtaaa aatagattgg
     4201 tgaaatatgc agcgtaaaat cttcccttgc gtcgcgtatt tcggcgtacc gccaaaaaag
     4261 tgcggcgaaa ctaaagagcg aagaagaaga gccacgggga ttgctctcgc tggggagata
     4321 tgttgtggtg gtataataaa aagggagtac gtacgttgta tcctccatca gcgtttggtt
     4381 tgcattgtgt gaaaagtgga gtaacgccca cacaccccac gtactttttt gttcgctgcg
     4441 cgcgcggtaa aaaagccagg tgtacgtatc tgtaaaattt tcggtgccgg tggtgatatg
     4501 caacggtgaa aaatggcggt acgcacgctt attattttct acccaccact tataccagaa
     4561 caatggaaaa atgcgatagc cgccttcgtt gggtgcgttg taagtattca caaacggcca
     4621 caagactgtt ttctccgacc aatttttttt gcagtcgtat tcgctccagt acacgatggc
     4681 gagccacgct tcggtactgc tcgtgcactc ggggaaaaat tgttgttgct ttccatagag
     4741 cggcaacagc gcggcgataa ttttatactc gacgctctcg tctttgtagt tgaaataaaa
     4801 cgggagaagt gccttaactc ctgtgtcgga atttttgtcc caggtgagcc aacttaaagg
     4861 cagcaagcgg atgtggcggt acgtgtcggc gtgttcgtac gcgaaaagtc caaagagaag
     4921 atagacaccg cgaaaatctt cgctgtgcgc acgatcgcgc atgcgcgttt gcgagagagc
     4981 tggcgtttgt ggaagtgctg cgtttgttgt cgcggtatct ttttcgtccg cgtcgagatg
     5041 gatactttcc cgccacgaga ggcgtaacag attgtagaac catccaaagt cggtgtcgac
     5101 aaggagagtt ttatcattca ccgacacgcc caccggcaag gcagaggcgt tttcctcggc
     5161 aaacgaaaat cctagcgcat cgatgtggag cgcatacccc tcgcgtcggt aacttaaata
     5221 aatcggcagt agcatttgaa aagaagaatc tgccgtgtcg cttttccaat agagcggaga
     5281 atagaactgc aatccttggg tctcgtggag cgcgaagtag agagggaata tggcgtggta
     5341 aaaatatttt tctgtgtcgg attttcccca gtacacaatc cacaacaacg aagtatcgct
     5401 ttcgcctgcg cgtgaaagcg tgaacgacac cggtgtgacg agggatgagt actctttatc
     5461 gagctgtcga tggtaccaaa acaagggagt aagtatcgag taagatttct tctcgtccgt
     5521 ggagtaataa tagaacggcg ccaacgcgtg atagcgcgaa tgtggacttt tttcttcccc
     5581 cgaataataa agccagaaga gagaaaaatc ccaactggtg atatcttctt ctttgtatga
     5641 aagcggcgtc aaaaaataag agcgcttatc gattttactt tctagcgatt tgaaaaacgg
     5701 gaacatccac gtactttcat agcgcggata atttttttgc ccccacagaa aaagtgcatg
     5761 ccacgcgtgg tgattttccc acgcttcgcg ctcgacaaaa aaaagccgca ccggatattt
     5821 ttcttcgcgc tcgcgcccgt acgcgcggct ttcgttttgg tctaaatccc cccacgaacg
     5881 tgcggaaagc tgcacagcac ccataaaaaa caaaagaatg agtgtgctga ctttcaaggc
     5941 aagctaagtt taacgatgtg cggtaagctg acaaatttat ttttcctttt gaaatttaga
     6001 gacggtcgtg tttcctcttg gcttgacatt atactggata catacgtata gtacgccaat
     6061 gtcagacaaa aaagtattaa attggagagc tgccatacac acgcctaaac tgttcttttt
     6121 ctgcgcagtt atctttttta gtcgctgcaa aaattttggt catttttggg attctcctcc
     6181 tgagcataag gcgaacactt ctgcatcggt cacgtattgg cgcaccgctg ataccggtca
     6241 aacgcagtgc tatgacaata ctacaacggc gacttgcacg ggcaacgctg gtagttaccc
     6301 caatcaagac gccgattttg tggatacccc gcgcgcacga tcttttacgg ggccgacaca
     6361 gcactctatt tattgggccg actacacgac ggtcgataat gtcacgggtc tcatttggaa
     6421 aacctgcacc gaaggcaaga cgggcgcaac gtgtaccgga accgcgggga actacacaat
     6481 ttcgcccgac acggcgacgc cggcatgcac cgctttgaat accgcaaact cgggcgtagg
     6541 ctatgcgggg cgcaccgatt ggcgcttgcc gacggtatcc gaattgcaga cgctcgccct
     6601 ttatagcgga gcaagtatcg cgatcgatgc gacggcgttt cccggagcgg tcgcgagttc
     6661 ctactggtcg tcgacgagcg atgtttatac acccgcaaat tcgtggttcg tgtcttacat
     6721 cgacggcgcc gttagtagta ctgcgaaatc tacggcgtat caagtacgct gcgtttcgcc
     6781 cgggccgcta cgcgcggcgg cgtataccga caatggggac ggtaccgtca gcgacgcttc
     6841 aaccggcctt gtgnnnnnnn nnncagcaat gcagcgccgg tctatcgggg aattcgtgta
     6901 cgggtacgtt gactccgctg gtttggtcgg cagcgctaac gtattgtaaa aatctcacgc
     6961 ttgcgggtcg ttcttggcgc atgccgagcg caaatgaaat tatgacgctc gccgattata
     7021 ccgcgacgaa cccggcgatc aatctgacgt attttccggg tacgcccgcc gccgagaatt
     7081 attggacgtc gacgacactg ccgggaaccc ccgcaaatgc acgccacttc tttattcagc
     7141 tgggtatatt gcataatatg gtaaaatcgg cagctataca aatacgctgt gtagctaatg
     7201 acctgtaggt taaggtatgg agcaagttag tttgatacgg ttttaactca tttaagactt
     7261 ttgggataat ctcaggttta ttcgctaatt taaattaagc cttcgtcctt gagtacttta
     7321 aaaaccgcag ggtctttttt catactttca aaaaacatag acacattttt taagtcgtca
     7381 aactggattt gcatagagtt acaccagttc aacatcacga aaaaataagg atcgacaatc
     7441 gaacgcacac cggcgaggta tacgccactc atgctcctat cgatgagttc caatttagta
     7501 cgcacttgtg tcgctgcatt cttcttcgac ttttcaatcg ctgctgcgtc ttctaaatat
     7561 gcagtcgaac caaaaaatgg cttaaaaaga ggatggagat cggcgttgat gattcctaag
     7621 tattggttga tttttgcttt tacttttaag ctaccgtcgc ccatgagttt tgcttcgggg
     7681 aatttctcag tcaagtaatg caaaatggcg ttgttctgag taatagtaaa gccgttgtct
     7741 tctaaaacag gcactgcacc gaaagggttt gcggcgacaa gttcgggtga cttggggtgc
     7801 agtccaactt tttgcagttc atacggttta ccaatccatt cgaggagaat gtgcgttgcc
     7861 atcgagcaag caccggaagc atagtaaagt ttcatgtgta ctattccttt gtgttttgtt
     7921 tttaataata tatgtcgaca taatttattc ttcaacaatt tatgtcttga aacaacaagc
     7981 gtatcgtgga aattgggtta tggcatacag acgaatgatt ggcgcgagcg ccagcgaaga
     8041 tagattagaa aagctcattg aagaccggct taaagaggga gcggataaag aaaaaattga
     8101 tagccgcatc tgggatcttt ttggcgaatc gtgggcggtg atgttcaccg atttatcagg
     8161 tttttcgcgc aacgtggcga agttcggtat tatccacttt ttacaaacta tttacgaatc
     8221 ggagcggttg ttaatcccca ttatcgaaga caacgacggt gtactgctca aagcagaggg
     8281 cgatagtctg ctcgttattt ttcgcaatgt taccaaggcg ttgcacgcat cgatttttat
     8341 gcaacgcact cttttagctt ataacaaaga taaaagcgac gaagaaaaag ttttgctttg
     8401 cgttgggttg gggtatggca aggtgttgcg tatcggcgat gtcgacgtgt tcggccacga
     8461 ggtcaactcg gcgtccaaat tgggcgaaga caccgccgaa gcgtacgaag ttttagtaac
     8521 gcccgctgta gcggagaacg cggcgaccca aaaagaattt aattttgaaa aaaccgaagc
     8581 agggcatttc aaacttttgt ggcggtaatc gactattacc taaaaattta cggaaatttc
     8641 caggtcgaac gtaaatcaaa agaaaaagtg aataccgacg catcgtagaa attttgaata
     8701 taccctaagt aaaaatagta gtaccgatgg ctcaccgtga aagtgagcgg cgaagcgata
     8761 gtgaaaatca tcgcattatt gagttcaagc cgattgcgcc attctccatt ggtcgatgtc
     8821 gcagaaaatg aatcaaatgc cagtttgtac gtgacgccac gaatgaactc ccactgaatt
     8881 ttgagcgttg cctcgatacc gccgaacgct acattcacag ggtctagaat ttgtttctcc
     8941 aaacccacac ccaagcgccc ggtaaaaata ccatacgaaa attcaccacc cgccgtttcg
     9001 cgcaaaaggc ttggacgcaa accggttgtg gggtctttca ccaccacggt atcgatgcgg
     9061 ttgcgttgat aagggttaat ataccattcg gtgttgtaag tgtatgtgac ttcaccccgt
     9121 aaaaggttac ggatgatgtc gttgctttga cgcatataaa aaatgtatgg attaaatgaa
     9181 aatgcatggc ggcggttgta aaaaagaaag ttgacgtcgt tttcaagccc ccatttaata
     9241 tacgaatcgg ttggttggcc gggcggagtt tcaatggctc ccggatttga gatttttccc
     9301 gactcataga tattcgagag gttgaaagtc gtcatcaatc taaaataaga gtcagcagtt
     9361 tgggcgggtg ttcgctcttc gttattctta gcaaagttca ccactgcatc gctaatgccg
     9421 aaccattgtg ttttacctcc caaagattct ccattttcac gctcagctaa ttcgtatccg
     9481 cgctgcgtag cacaaacacg gtacttaact ttttcgcgta taggataccc cgttatttta
     9541 tttttatcaa accctgaata aacgagtcct ggatattttg tatagaattt acgtagcgct
     9601 tcaccggtca tgccgaatac aaagatatta taatcgttct cgacgttgta gcgaatttgg
     9661 tttgcgtcgg ttagcgtaga attgtcggta acggcaatgt cagaagcttc gatgaaagac
     9721 acatcgcaat tcgactcttc acgcaccgcg tcggcagcaa attttggcgt caatgtaacg
     9781 ggcgagaaac ttgcgtcgcg cacgccgttt gcatcggtgg tataccattt ttgccaaagc
     9841 ctaagccggc gcgtaaaaga tggcggtggc tccgcacttt taggaatatc gagcaggcca
     9901 acattttctt gctcggtgag ttgccagccg ctgcgggttt gctttaaatg taccttggtg
     9961 agcgatgcct cgggcggcag cgcaataagc cgtcgcccat cgggcaattc aatcgtgcgg
    10021 atagggcttt gccccaacgc actttcagcg tcgccaccac aaatcatgat gtcgatacgt
    10081 cgatgttttg cgagtaattg aatggcgtcg cttactgaaa cgctggtcag cactacgaca
    10141 atatcaggac tctcagtcga tacgcgttca atagtttctg tcaatacatc ttttgggagc
    10201 gccagcctca attcgagcgc ttcggcttct tcataagcga caagcgagcg cgccgaggtt
    10261 aaccccacaa tggcagcctt tacttcccct tttttgataa ctgtgtatgg ttcaaaaaga
    10321 ggagtcttgt cacggaaaat attcgcagaa acaagccgta taccgcgagc cctgcggatg
    10381 tactccaaac tttcagcgcc aatgttgaga tcggcggcgg caacgatgcc tgcgtcaagg
    10441 cgcaccatct gaaaataatc ggcggtgagc gaaccaaaac taaaacgcga aagtctgcct
    10501 ggataaaaag cgttgcctaa atcaaaatga tatgcattgg gataactttc tttggcggcg
    10561 cgtagcgcac ccgcaatacg aatgattgtg ctgtcggcat ctttttcatt tgcgcctatc
    10621 ggaaaacgac cagcaagatt tgaagttaaa tagaacgtta cctctttagc ttgtgcagca
    10681 agcgcacaaa aaagtgcaag cgttatatga acgaaaaaaa tacgcattaa tgtggcgctg
    10741 ttttactttt taactctgaa agttcccgcc gcagcagctc aagctctttt tcttgtgcga
    10801 attgcttatc ggcagcttct ttttgcaatt tttctttttc tttggccgct ttttgttgag
    10861 cgtctgccag ctcgtcttgt aattttttct gtgcctcgat tgatttttga gtcaactgtt
    10921 cattgagttg agtgagttta cgaattttac ccacgctgtc cgattccaaa attgcgattt
    10981 tatcggttag attagtgagg tgcgtttcta acgctttcat gctggctgct tgtttttctt
    11041 gctcagcgac gaagcttgcg gctttttttt ctgcttcggc tttggctacc tctaccgctc
    11101 gtgtgagttc taagttttcg tcgcgtagaa tttgattttc gcgttcgagt aacgcggttt
    11161 tacgttgaat gagcgcacgt tctgctgcga tttgatcgct tgaacgatct tgccaactgg
    11221 cgcacgaagc taaaaaaaag acgaatagaa aaatcgacaa tgcgtgcatt agtcgtagaa
    11281 attggtagcg cataaataaa attaaaaatg aatcgccaca ccttcaatac gagataattt
    11341 gcccatcact gtcgcctttg gaaagatttc acgtatcgcc atcaattcgc tctcttcggc
    11401 gattaaaata atgccgacac ccatgttaaa tgtgtggaat aatttttcgc gtggaacaaa
    11461 tttctcaagc gtttcaatcg ctttaaggcg aaacggttct gggttctcaa tcactgcgcc
    11521 caacccagat gcaatgatac ggggcaaatt ttcgtgaatg ccaccgccgg tgatgtgggc
    11581 aattcctttt atcggtacct tttctaataa gcgggggatt tcatgataaa tacgcgtggg
    11641 ttgtaaccag gtttcaatga gcgctgcgcg attcgccacc atcgtctgaa aaccaatggg
    11701 atcacttttt tcaatttcat taagaatttt gcgcactaaa gaaaaaccgt tggaatggag
    11761 tcccgacgat ggcactccca ccaaaatgtc tccggctttt tgtgtttcaa aattaggcag
    11821 aacccgcgag cgtggcacaa agcccactgc aaaaccggct agatcaaaat catcgtcggg
    11881 cattacaccc gggtgttctg cggtttcgcc acccatgaga gccatcggcg attgcgcgca
    11941 tgcgtcgaca atgctcgaca gaacacggtt ataccatttt tcatctaaat gaccgcacgc
    12001 gatataatct aaaaaaaatg ccggtgtcgc tcccgaacaa aaaagatcgt tgacgcacat
    12061 tgccaccaaa tcttgtccaa tgccttcgag ataattgaac cgacgcaaca gctgtgtctt
    12121 tgtgccgacg ccatcggtcg ccgctacaac gtcaaagcct ggaatttgta ccgcttcaaa
    12181 acggccagca aagccgccat atttacccac gacagttcca acgcgaggca ctcgatgtgt
    12241 tgccgaaatt ttatctttca gtttgtggat taactgttgc gcatagtcgg tatcaacacc
    12301 ggaagattta taatccatgc gggagttaaa attactcaag acagggtgaa aaaaacaaaa
    12361 ttacttccat tttgaagttc tttattttct caggcgtagc gcgttgacaa tcaccgagac
    12421 cgagctaaaa ctcatcgccg ccgcaccgat catcggtgaa agtaaatatt gaaaaatggg
    12481 tacaatactc ccgccgcaat aggaactccc aacaagttat acacaaacgc gaaaaataaa
    12541 ttttggcgga tgttgcgcat cacgcgtcga ctcaatccga gtgcagcggt aatcttgttc
    12601 aagtcgccgc tcgccaaagt gatgcctgcg ctctccatcg ccacgtcggt gccgtgcccc
    12661 atggcaatgc c
//
LOCUS       DAOMPD010000013        12470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2191_length_12470_cov_2.558840, whole genome shotgun sequence.
ACCESSION   DAOMPD010000013 DAOMPD010000000
VERSION     DAOMPD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_12470_cov_2.558840"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1198
                     /locus_tag="PLY93_01025"
     CDS             104..1198
                     /locus_tag="PLY93_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68100.1"
                     /translation="MLYRVRLLALLSLPFLISCARFRTSTLKPEKIFAATIGSEKIVY
                     PKQSGIYHEIPSRVGVHKEWIALSEPSEKTIKLFRSGKLEAVIRAQDSGKTKNDAKGL
                     EALRIPGRIMMGVNDDFFVENYIPPEGENKEASSRGGYKILQFDFKGKLLRSIGRKGL
                     PELVFENLLWFDADSKGYLWVLYRYLDELHLDRYEGSRLAYEYRQTDCENATVDKKQI
                     EQLKSRNSIVHCEYIYPFYSGERIMFASRIDRIDTESDKKQMALAYRVVKVKNLGEAE
                     NKTVFDRLANAEDHPYLPQGGNYVGIWQNLENGRVRVQRYSLDGDLTNSIVLDMTGRV
                     SQWRQVWSTLSGNFYGIRIFSDQFEIYKFQ"
     gene            1285..2210
                     /locus_tag="PLY93_01030"
                     /pseudo
     CDS             1285..2210
                     /locus_tag="PLY93_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
     gene            complement(2442..2915)
                     /locus_tag="PLY93_01035"
     CDS             complement(2442..2915)
                     /locus_tag="PLY93_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRP68101.1"
                     /translation="MQVTRNKVVTLEYEVAEKDKGKLENGTLDYLHGFKNLMPALEKF
                     LTDKKEGFSGKVEMGPRDAFGEVNPDLVMEIPKAQFDTPEELKEGMQIELESEEEGII
                     PALVTKIEGDTIHVDGNHPYAGKTITFDVKILKIRDASTEELAHGHAHGAHGHHH"
     gene            complement(2915..3613)
                     /locus_tag="PLY93_01040"
     CDS             complement(2915..3613)
                     /locus_tag="PLY93_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68102.1"
                     /translation="MKANKAKDKKSAKIKTTQGEIRKRQSINAVKKQKTTSLSKKNAS
                     SNLPLAEPEKLDTQMSPNIIDEFAIANAWEILGDEEYILDDSIWNTLYSRVKLQLLQT
                     TYSFKVENEGTTLSPYAVTPMPAFRAESAPGYLQAGSFTILRPELPGVVFDLKITSGA
                     SSLLLDWQLLRKDTKSGTGHIALYINGILVEAANLQQSRCRLALQREDIGDATFYFLD
                     ADTGRQAKILELNL"
     gene            complement(3642..4490)
                     /locus_tag="PLY93_01045"
     CDS             complement(3642..4490)
                     /locus_tag="PLY93_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68103.1"
                     /translation="MNETDFQALFNAYRTAALAQKENPVPPALHLMVYNYARIRHQQS
                     SDVSGDFYLHVSNKLEGILHRYDASLLPFYQYMAQTLNFEFGHFLRRRKLPRSQIRLQ
                     SIEELREKRVELFENTDAASLTKEDILDAVIQSVPLKNQKYAKIALALPLTYADLRAI
                     IHKQQNNKNTAWQLLRAYREYLHFIEKKRHETQRKRDRLIETLMHLAHELTTASGKTR
                     TRLTQRKDATEKKFFSIDTRIPIRIVAATMGDSIATAQRQLKSAVIALRKAWLQREKQ
                     KLDTKK"
     gene            4621..5490
                     /gene="dapF"
                     /locus_tag="PLY93_01050"
     CDS             4621..5490
                     /gene="dapF"
                     /locus_tag="PLY93_01050"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRP68104.1"
                     /translation="MSGSKALRFAKLEGLGNDYLFTEDSPKNPQRAARLLCDRHYGVG
                     ADGLVLIMQPDNKNEADFQIRIFNPDGSEAEMCGNAIRCLGKYLYDRGNTKKQEINVQ
                     TLRGIIPCILMVRDGRVYRVKADMGEPILERAKIPMQCAAGEEEKKVLKEKIYLEDHN
                     TFEFMALSMGNPHVVIWVEDVDNFPVAKYGPLIENYSLFPNRTNVHFIQMLDEDHIKQ
                     RVWQRGVGETLASGTGACASVVAGVLAGKIKRKSKVEMPGGILEIYWSEKDNHVYMTG
                     PCKDVFEGQTLLI"
     gene            5451..7082
                     /gene="purH"
                     /locus_tag="PLY93_01055"
     CDS             5451..7082
                     /gene="purH"
                     /locus_tag="PLY93_01055"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRP68105.1"
                     /translation="MQRRFRRPDIVDLITPLDLQPIKRALLSVSDKTGLEDLARVLSE
                     RGVELLSTGGTLKFLREKGFTATAVEDYTGFPEMMDGRVKTLHPKIHGGFLARRDVPE
                     HLAAAVKHQIQLFDLVVVNLYPFESVIAKVETTFEEAIENIDIGGPAMIRSAAKNAAS
                     VTVLVSSTQYDALIQNLAAHNGATTRAFREECRLAAYRRTGEYDAAISAYLTARNNGN
                     ITESAPSTAPLQNVSLTLHEVQPLRYGENPHQSASYLVAKDADKIWQQRAGKELSFNN
                     ILDLDIAIRLAADFTQPVCAIFKHTNPCGVATGENQAQILQYAIDCDPVSFFGGIVVF
                     NRTLTLDAAKKLAPHFLELIIAPAFDAEALTLLAAKKNLRLIEVNFNRADKMHAKDLR
                     AVPGAGGYLLQEQDSALWQKENLKTVSKKEVDAATLADLEFAFRVAKFVKSNTVVFAT
                     GGRTLAIGAGQMSRIDAIHIAQKKAGEAKLSLKDAVMASEAFFPFRDSVDTCAQIGVR
                     AIVQPGGSIKDADSIAAADEHGIALVFTGMRHFRH"
     gene            7095..7304
                     /locus_tag="PLY93_01060"
     CDS             7095..7304
                     /locus_tag="PLY93_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68106.1"
                     /translation="MKDYHVNIFYSDEDEGYIADIRDLNHSSTFGNTPEEELCRVGTA
                     KTLWFETAKKIRKRYRLRYTNPRFI"
     gene            7394..8707
                     /locus_tag="PLY93_01065"
     CDS             7394..8707
                     /locus_tag="PLY93_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68107.1"
                     /translation="MSYFVDETLKEKFKPHVFFVWGVLPGERFVARLAKVKSKAAHGI
                     LARHDELLEEEKNKSTSEKNPTHLYSTPAFCHPTWALYLASKERLEPDCENFLRCGGC
                     KMRHVTYETSLRYKTEWLKTQLTHHKIPTDFLAVDYTDASERVHYRNHIQVHINKFGV
                     YGFYEPLSYHTRAFPEHGCLIFDEKSIRGEFPKFPLEVRTARIRQNIDGRSVFTVFNS
                     KEERSTTMRYTIAWPPHTHTHIDFPVTQFFQVHLRLLPRWLQTIASYLSSAISPKDAP
                     IRVLELFSGFGFISRMLAQVFNLEVVAADLLQKADVERVVLTSDAGEKLTEDFHKNYH
                     RVDLFLPERLDEKFLESVERHNPEVLLVNPPRAGLAEATWNALRQKALCNFSGPIIYS
                     SCNTATLARDLAYLLQEGWQIRQMQLFDFFPWTHHSETVVLLAKR"
     gene            complement(9134..9658)
                     /locus_tag="PLY93_01070"
     CDS             complement(9134..9658)
                     /locus_tag="PLY93_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP68108.1"
                     /translation="MKKILAIAAAMAISTAAFGANVKANKLGVNFAYDSGTSNIGVLW
                     HVADTVALRPFVGFKNKAIDQGTTTTTMTFGLDLPIYLANFNALDLYVAPGISYNSEK
                     TAPAIGSATTATTLDLKAMLGLQVKLADQVHLFGELGLSYSSYNDGTPAPGTTKSTTI
                     ATGRTAVGAIFYFN"
     gene            complement(9903..10769)
                     /locus_tag="PLY93_01075"
     CDS             complement(9903..10769)
                     /locus_tag="PLY93_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRP68109.1"
                     /translation="MQINNNLSAMAVNRHLHKVYGNFEKSIERLSTGERINRAGDDAT
                     NLAVSEKMRTQIRGLKQAERNAMNGLSFIQTAEGNMGQVNDILQRIRELAVQSANGIY
                     SDSDRQQIQVEVSQLVDEIDRIGTSAEFNRMKLLTGRYARGSKLGSIFFHVGPNKDQR
                     IQAFIKTMNGRAFGFYEGLGAKSAKKPLATVFQANEMIGTVDNAIDKLVRQRADLGAY
                     YNRIETTIDALRQGYENMVAADSRLRDTDMAEELLEFTKNQIQLQSGTAMLAHANFSS
                     RLVVSLIEHGSK"
     gene            complement(11173..11655)
                     /locus_tag="PLY93_01080"
     CDS             complement(11173..11655)
                     /locus_tag="PLY93_01080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRP68110.1"
                     /translation="MPSPLPYRPNVGIVVFNKEGLTLVGERLDNPGAWQYPQGGVDAG
                     EEFVVAAQRELAEETGIRDALFIFEAPELLYYNFPEGLVIPQMTDRYRGQMQKWYLAY
                     WNHPTNDTNLHTHTQEFARVAFRPMAEATNQIVPFKRTIYAALERLFLPKIQQFLQKL
                     "
     gene            complement(11709..12287)
                     /locus_tag="PLY93_01085"
     CDS             complement(11709..12287)
                     /locus_tag="PLY93_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68111.1"
                     /translation="MKLLKTLAVCVVAASFIACGSGNVRFPDKTTKIDLKNKSVLVVV
                     EDNASSDTKVSLAFKGAAAEKVGSKKVLPTIPDSVKGVTKIYTNFGLQDKGVVDPQNL
                     KALAIDEILKLSAKLGKFDTIVFVSAEKGSGMAVPQTTGVEFYASVYDIAGKKVLAAV
                     SDSASLVTTGLLAQMPLKARDIVTVLLEGQAD"
BASE COUNT         3114 a         2816 c         3048 g         3492 t
ORIGIN      
        1 aaattgtgga tttaccgacg ttgggtagtc caacaatacc gcattgtaat ggcatgagcc
       61 taaagtgatg tccgcgacag gtcgacaatt caaagcggct cgcatgcttt atcgcgtacg
      121 cttgctggct cttctttcac tgccttttct catctcatgc gctcgctttc gtacaagcac
      181 gctcaaacct gaaaaaatat ttgcggcaac gattggttca gaaaaaattg tctatcccaa
      241 acaaagtggt atttaccacg aaatacccag ccgcgtgggg gttcacaagg agtggattgc
      301 cctctctgaa ccttcggaga aaacgattaa attgttccgc tcgggtaaac tcgaagcggt
      361 gattcgcgcg caagatagcg gtaaaacgaa aaacgatgcg aaagggcttg aagcgttgcg
      421 tattcccggg cgcattatga tgggggtgaa tgacgatttt tttgtcgaga attatattcc
      481 tcccgaaggc gaaaataaag aagcgtcgtc gcgcggcggt tacaaaattt tacaatttga
      541 ctttaagggt aagctcttgc gctctattgg acgtaaagga ttgcccgagc tggtgtttga
      601 gaacttactc tggttcgacg ccgattccaa agggtatctt tgggtgctat atcgctattt
      661 ggacgagttg catctcgacc gttacgaggg cagtcgtttg gcgtacgagt atcggcaaac
      721 cgactgtgaa aacgccactg tggacaaaaa acaaatcgag cagttgaaaa gccgtaattc
      781 catcgtgcat tgcgagtata tttatccttt ctattcgggg gagcgcatta tgtttgcctc
      841 gcgtatcgac cgcatcgata ccgagtcgga taaaaagcaa atggcgcttg cgtatcgcgt
      901 tgtcaaagtt aaaaatttag gcgaagcgga aaataaaacc gttttcgacc gtttggccaa
      961 cgccgaagac cacccctatt taccccaagg gggtaactat gtcggtattt ggcagaattt
     1021 agaaaatggg cgggtgcgtg tgcaacgcta ctcgttagac ggcgacctca ccaactcgat
     1081 tgtgcttgac atgacaggtc gagtaagcca gtggcggcag gtttggagca ctctttcggg
     1141 taatttttat ggtatacgta ttttttcaga ccaattcgag atatataaat tccaatgaaa
     1201 caatttttga ttaaagagtg ggggcttttg ctctcggttg tggttttcac gggtgtgatg
     1261 attgccgact accatttcgg ttttatggca cactggacac cgctcacctc gggacgaact
     1321 tccgatttat ttttatcgtc gggaatcgtc acggttgtca ccttggcgtt tcttgaaggg
     1381 atattgggaa tcgataacgc cactgtgctc gccatccaag tgcggcacct aaaacccgac
     1441 gacgccgaaa aagcgctttt gtggggggtg tggggggcgc acctttccga tttttatttc
     1501 tcattgccgc cggtttcatt ctcgagcaaa agtggattat ggctttgggg ggcttttacc
     1561 tcgtgaacat ggcatcgatt tttttcttta accgctatat tgttttctta atcgatatac
     1621 ttttcttagg cattgttctt acagtctttt attttacaaa agatacgctc acgatttcaa
     1681 cgctgtcgat acccgtatgg atatttttta tcccgctggt tttatactcg gtgtatcgtt
     1741 tttggaaaca tagcaaccca gctttgaata aaaagaaaaa ggaatcccat gaagaggaag
     1801 aaggattgca agtctcgcgt tttaagtgga aaggacacgc tattttagcg gcgattatcg
     1861 ccgtcgagtg gacggatatt ttattttcat tcgactcgat tggtgcaggg gtcgcgatca
     1921 cgcgcgactt ttgggtactt ttctgggggg cattttttgg agttacctgc ttacgccttt
     1981 ttgctaaaaa atttgtgaag ctactcgata gattcccaca gttggaagcc gcggcgatgc
     2041 ttgcggtact tatcgtgggt gtgaagatgt cttttgaagc ggtgcaggat gcactacagc
     2101 ttcatcttta ccatatcaag aactggcaaa ccagtttagt gattattgtt atttttattg
     2161 gggcgttttt ctttaaggca aaaacgccgc acattagcga agaaaaataa gttacgagcc
     2221 gggtattttc tggggttgtc caataggccg gtcaccgagt gattcgtcgc ccacctccga
     2281 agacggagag cgaagacgct cacgatacaa aaaccttagc aaatgctaag gttttttact
     2341 cggcgaccag cgacgaatcg tatcgcgacc cgtcgctcgc accgagcaaa gtcgaggtgt
     2401 cgggtctctc gcagagaggt gactttttgg acagccccta gttaatgatg gtggccgtgt
     2461 gcgccgtgcg cgtggccatg cgccagttct tctgtcgaag cgtcgcggat tttgaggatt
     2521 tttacgtcaa aggtgatagt cttgcctgca tacgggtggt tgccgtcgac gtggatggtg
     2581 tcgccttcga ttttagtgac gagcgcgggg ataatccctt cttcttcgct ttccaattca
     2641 atttgcatcc cttctttgag ctcttcgggg gtgtcgaatt gagccttggg gatttccatc
     2701 actaaatctg ggttcacttc gccgaaagca tcgcgtggcc ccatttcgac tttgccggaa
     2761 aagccttctt ttttatcggt gagaaatttt tcgagcgcgg gcatgagatt tttgaagccg
     2821 tggagataat cgagagtgcc gttctcgagt ttacctttat ctttttccgc tacttcgtat
     2881 tcgagagtga ctactttgtt ccgtgttact tgcattatag gttgagttcc aaaatttttg
     2941 cctgtcggcc tgtatccgca tcgagaaagt agaacgtggc gtcgcctata tcttctcttt
     3001 gtaacgccaa acggcagcgg ctttgttgta agtttgccgc ctcgacaaga ataccgttga
     3061 tataaagggc gatatgccct gtacccgact tggtgtcttt gcgtaaaagt tgccaatcga
     3121 ggagtagtga agaagctccc gacgtgattt tcaaatcaaa gaccacccca ggaagttctg
     3181 gacgcaaaat ggtaaagctg cctgcctgca aatagccggg ggcgctttcc gcacggaatg
     3241 ccggcatggg cgtgacagcg tagggggaca gcgttgtgcc ttcgttttct actttgaatg
     3301 aatacgtggt ttgtaaaagt tgtaatttga cgcggctgta aagcgtgttc caaatgctat
     3361 cgtctaaaat atattcttca tcgcctaaaa tttcccatgc gttggcgatg gcaaattcgt
     3421 cgatgatatt gggggacatc tgtgtgtcca gtttctctgg ctcggcgaga ggcaaatttg
     3481 acgatgcatt ttttttagat aaactggttg ttttttgttt tttcaccgcg tttatgcttt
     3541 gacgcttgcg gatttctcct tgcgttgttt ttatttttgc tgatttttta tctttcgcct
     3601 tgtttgcttt caaaatacct cgctaccgta ctgtatacgt tttatttttt tgtatccagt
     3661 ttttgttttt ccctttgcaa ccacgctttg cgtaaagcaa tcactgcact ctttaactgt
     3721 cgttgcgcag tggctattga atcgcccata gttgccgcca cgatacgtat agggatgcgg
     3781 gtgtcgatgg aaaaaaactt tttttcggtg gcatctttgc gttgagtgag tcttgtgcgg
     3841 gtttttccgc ttgccgttgt caattcgtgc gcaaggtgca tcagcgtttc gatgagacga
     3901 tcgcgctttc tttgggtttc gtgccgcttt ttttcgataa agtgcaagta ttcgcggtaa
     3961 gcgcgcaaga gctgccacgc ggtattttta ttgttttgtt gtttatgaat aatggcgcgt
     4021 aaatcggcgt acgtcagtgg gagtgcaagc gcaattttgg catatttttg atttttaagg
     4081 gggacgcttt gtattaccgc atcgagaata tcttctttag ttaatgaagc cgcgtctgta
     4141 ttctcgaaaa gttcgacccg tttttcgcgc aactcttcaa tcgattgcag ccgtatttgc
     4201 gaacggggga gtttacggcg ccgcaaaaag tggccaaact caaaattgag ggtttgcgcc
     4261 atgtactgat aaaacggtag caaagatgcg tcgtagcgat ggagtattcc ctcgagctta
     4321 ttcgagacgt gtaaataaaa atctcccgaa acgtcgctcg actgctggtg gcggatgcgt
     4381 gcatagttgt ataccatgag atggagcgct ggcggtacag gattctcttt ttgagctaaa
     4441 gcggcagtac ggtacgcatt aaataaggct tgaaagtcag tctcgttcac agtatttggt
     4501 ctgctattat cggcattcta caaaagtttg aaaaaattgc aacagtttta tcaaaaataa
     4561 caaatccagt taacgaatga aacaaataga aattgaaaag ttaaaattaa ggagatttac
     4621 atgagtggtt ctaaagcatt gcgttttgcc aaattagaag ggttgggtaa cgattatctt
     4681 tttaccgaag attctcccaa aaacccacaa cgagcggcgc gccttttatg tgacaggcat
     4741 tatggagtag gggcggatgg cttagtgctc attatgcaac ccgacaacaa aaatgaggcg
     4801 gattttcaaa ttcgcatttt taaccccgat ggctcggaag ccgaaatgtg cggtaacgct
     4861 atccgttgcc ttggcaagta tctatacgac cgcggtaata ccaaaaaaca agaaatcaac
     4921 gtgcaaaccc tgcgcggtat catcccttgc atcctcatgg tgcgtgacgg tcgcgtgtac
     4981 cgcgtgaaag ccgacatggg tgaacccatt ttagaacggg caaaaatccc gatgcagtgc
     5041 gcagcgggcg aagaagagaa aaaagttctc aaagaaaaaa tttatctcga agatcacaac
     5101 actttcgagt tcatggcgct ctccatgggg aatccacacg tcgtcatttg ggtcgaagac
     5161 gtggataatt tccccgtagc aaaatatggc ccactgattg aaaattattc gcttttcccc
     5221 aaccgtacca acgtgcactt tatccaaatg ctggacgaag atcacatcaa gcagcgtgtg
     5281 tggcagcgcg gcgtgggcga aactcttgcc tcgggcacgg gcgcgtgcgc gtcggtcgtt
     5341 gcaggcgtgc tcgcaggcaa aattaagcgt aaatccaaag tcgagatgcc cggcggtatt
     5401 ttggaaatct attggtcaga aaaagacaac cacgtttaca tgactggccc atgcaaagac
     5461 gttttcgaag gccagacatt gttgatttga ttaccccgtt ggatttacaa ccgataaaac
     5521 gcgcgctgct ttctgtttcc gataaaacgg gactcgaaga tttagcgcgc gtactctccg
     5581 agcgtggcgt tgaacttctt tcgaccggcg gtacgcttaa gtttttgcgc gagaaaggtt
     5641 ttaccgccac cgccgtagaa gactacactg gttttcctga gatgatggat ggccgcgtga
     5701 agacattaca ccctaaaata cacggtggct ttttggcgcg ccgcgatgtg cccgagcatt
     5761 tggctgccgc ggtaaaacat caaattcaac tttttgattt agtcgtggtg aatctttacc
     5821 ccttcgagag cgttattgca aaagttgaaa cgacgttcga ggaagccatc gagaatatcg
     5881 acattggtgg tccggcgatg atccgctcgg cggccaagaa tgccgcttcg gtgacggtac
     5941 tcgtttcttc cacacaatac gatgcgttaa tccaaaacct tgcagcgcac aatggcgcca
     6001 ccacgcgtgc ttttcgtgaa gaatgccgtt tggctgcgta ccgccgcacc ggcgaatacg
     6061 acgcggcgat cagcgcgtat ttaaccgcac gtaataatgg aaatattaca gaaagcgctc
     6121 cctcaacggc gccgctccaa aacgtttcgt tgacgttgca cgaagtgcag ccgttgcgct
     6181 acggtgaaaa tccacaccag agtgcatcgt atttggtggc caaagacgcc gacaaaattt
     6241 ggcaacagcg tgcagggaaa gaactttctt tcaacaacat tcttgattta gatattgcca
     6301 tccgcttggc tgccgatttc acgcagccgg tgtgcgcgat ttttaaacac actaacccct
     6361 gcggcgtggc gacgggtgaa aaccaagcac aaattttaca gtatgcgata gactgcgatc
     6421 cggtgagctt ttttggcggc atcgtcgttt ttaaccgtac gctcacgctg gatgcggcaa
     6481 aaaaactcgc gccgcatttt ctcgaactca tcattgcgcc ggcattcgat gcagaagctt
     6541 tgaccctcct tgcggcaaag aaaaatttac gcctcatcga agtgaatttc aaccgtgcgg
     6601 ataaaatgca cgcaaaagac ttacgcgctg tgcccggtgc tggtggctat ttactccaag
     6661 agcaagatag cgcgctttgg caaaaagaaa atttgaaaac agtttctaaa aaagaagtcg
     6721 atgccgccac tctcgccgac ttggagtttg ctttccgtgt ggcgaaattt gtgaaatcta
     6781 acaccgtcgt ttttgccacc ggtggtcgga ccctcgccat cggtgcaggc caaatgagcc
     6841 gcatcgatgc aatccacatt gcgcaaaaaa aagcgggcga agcaaaattg tcgctcaaag
     6901 atgcggtgat ggcgtccgaa gcattttttc cgtttcgcga tagcgttgat acctgcgccc
     6961 aaatcggtgt gcgcgcgatt gtacagccag gcggttcgat taaagacgcc gacagtatcg
     7021 ccgccgccga tgagcatggt attgcgttag tttttaccgg tatgcgtcat tttcgccatt
     7081 aatgtgcgaa tagaatgaaa gattaccatg tgaatatctt ctatagcgat gaagacgaag
     7141 gttatattgc agatatacgc gacttaaacc acagttctac ttttggtaat actcctgaag
     7201 aagaattgtg cagagtggga actgccaaaa cgctttggtt tgagactgct aaaaaaataa
     7261 gaaagcggta ccgactccgc tatacaaacc cgcgtttcat ttagcggtgg gctgaattat
     7321 caacatcaga aaactcaaac ctggaacctt cgtagagctc gaatgcaagc acttcgtctc
     7381 cgaaggcgtc ggcgtgtcgt attttgtcga tgaaactctc aaagagaaat tcaagccgca
     7441 tgtttttttt gtttggggtg tgctgcccgg cgaacggttc gtcgcccgtt tagccaaagt
     7501 gaaatcgaaa gcggcgcacg gcattttggc gcggcatgac gagttgctgg aagaggaaaa
     7561 aaataaaagc acttccgaaa aaaatcccac ccacctttat agcacaccgg cgttttgcca
     7621 tcccacttgg gcgttgtatt tagcgtcgaa agagcgcctc gaacctgact gtgaaaactt
     7681 cttgcgttgc ggcggttgca aaatgcggca cgttacgtac gagacttcgc tgcgttacaa
     7741 aaccgagtgg ctcaaaacgc aactgacgca ccacaaaatc cctacagatt ttcttgccgt
     7801 cgattatacc gacgcaagcg aacgcgtcca ctaccgcaat cacattcaag tgcatatcaa
     7861 caaatttggc gtgtatggct tttacgaacc gctctcgtac cacacccgcg cattccccga
     7921 gcacggttgc ctcatctttg acgaaaaatc gatacgaggt gaatttccga aatttccgct
     7981 tgaggtgcgc actgctcgca ttcgccaaaa cattgacggg cgcagcgtct ttaccgtgtt
     8041 caacagcaaa gaagagcgtt cgaccacgat gcgttatacc atcgcctggc cgccgcacac
     8101 gcatacacac attgattttc cggtaacgca attttttcaa gtacacctgc gcctcttgcc
     8161 acgctggttg caaacgatag cgtcgtattt gtcatcggca atttccccca aagatgcgcc
     8221 catccgcgtg ctggaacttt tttcgggctt tggatttatt tcacgcatgc ttgcacaggt
     8281 ctttaatttg gaagtcgtcg ccgccgatct tttgcaaaag gcggatgtag agcgtgtcgt
     8341 gctaacatcg gatgcaggtg aaaaactcac agaggatttt cataaaaatt accatcgtgt
     8401 cgatttattt ttacccgagc gtttggatga aaaattttta gagagtgtcg agaggcacaa
     8461 ccccgaagtg cttttagtca atcccccgcg cgcagggctc gccgaggcga cgtggaatgc
     8521 actgcgccaa aaagcgcttt gtaacttttc cggtcccatt atttattcgt cgtgcaacac
     8581 tgccacgctc gcgcgcgatt tggcatacct tttgcaagag ggttggcaga ttcgacaaat
     8641 gcaattgttt gatttttttc cgtggacgca ccattcggaa acagtggtgt tgttggcaaa
     8701 aagatagcct tacggaagcc aacgcaataa acttccgtaa cgctatccat gcctatgcac
     8761 gtcaaaaatg gataggctgt gcgtataaag ggaagttttt tctttaaagt tttccgattt
     8821 taaccagtcc agtaccgtgg tcatagttat cccttcatgc gacataatcc gtaaaattgg
     8881 gatagttatc cctggcaact tgtcgcgtcg tttctaaatc tgctttaatg ccagctcgtt
     8941 ctcgcgtatt gaagtgatat ggttgtgatc tctttgcggc cttgtgggca ttttactcga
     9001 ctttaagcga cggtagattg taagactacc catcgagagt agtttggggg gtaaatatta
     9061 aactaaaaga aaagcccatt atcggatgcc aagcatccga taatgggcag aaagcgtgtt
     9121 ctacgtggtt aaattagttg aagtagaaaa tcgcgccaac ggctgtacgg cctgtcgcga
     9181 tggttgtgct ttttgtagtt ccaggagctg gcgtaccatc gttatagctg ctgtaggaaa
     9241 gtcctagttc gccaaaaaga tgcacttggt ctgcgagttt cacttgaaga ccgagcattg
     9301 ctttaagatc taaagttgtc gcagttgtag cactgcctat tgcaggtgca gttttttcag
     9361 aattgtatga aatccccggt gcaacgtata gatccaatgc attgaaattt gccaagtaaa
     9421 ttggtaaatc aaggccaaat gtcatcgttg ttgtggtagt accttgatca atagctttat
     9481 tcttaaagcc aacaaatggg cgtaatgcaa ctgtgtcggc gacatgccaa agaacaccaa
     9541 tattcgaggt gccgctatca taagcaaagt tcacacccaa tttattcgct ttcacattgg
     9601 caccaaacgc tgcagtcgaa attgccatcg ctgccgctat tgctaaaatt tttttcattt
     9661 gaactcctaa aagttattta ttcaatcgag gcactttgcg tctcgattga atatatacta
     9721 atttatagcg tgcagttgta aatcaatttt ttaggtaatt gttgactctt tgtcaacgta
     9781 tggttatgct atccccatga cgaacgacga actgcgcgag ttgcaataaa aaaaaacgga
     9841 tagcggacgg ctgcgtaagc ggctgtccgt tatccgtggt agtgaaccct attcagtttc
     9901 tattacttgc tgccgtgctc gatgagcgaa acaacaagcc gagaggaaaa gttggcgtga
     9961 gccaacatcg ccgtaccact ctgtaactga atttggtttt ttgtgaactc cagcagttct
    10021 tcggccatgt cggtgtcgcg cagccgcgaa tccgccgcca ccatattctc atagccttgg
    10081 cggagcgcgt caatcgtggt ttcaatacga ttgtaatacg cccctaaatc tgcccgctga
    10141 cgcacgagct tgtcgatcgc gttgtcgacg gtgccaatca tttcgttggc ttgaaaaacc
    10201 gtcgcgagcg gttttttagc cgatttggcg cctaagccct cgtaaaaacc aaacgcacgg
    10261 ccgttcatcg ttttgatgaa cgcttggatg cgctggtctt tgtttgggcc tacgtgaaag
    10321 aaaatcgaac ctaattttga gccacgcgcg taacgcccag tcaaaagttt cattcgatta
    10381 aattcggcgg atgtaccgat gcggtcgatt tcatcgacca gttgcgatac ctctacctga
    10441 atttgctggc gatccgaatc ggaataaatt ccgtttgcgg attgtaccgc cagttcacgg
    10501 atgcgttgca gaatgtcgtt gacttgcccc atgttgccct cggcagtttg aatgaacgaa
    10561 agcccgttca tcgcattgcg ctcggcctgt ttcaagcccc gaatttgcgt acgcattttt
    10621 tccgacaccg cgagattcgt cgcgtcgtcg cccgcacggt taatccgttc gccagtcgat
    10681 agcctttcaa tcgatttctc gaagttgcca tagactttgt gcaaatgccg atttaccgcc
    10741 attgcggata gattgttgtt gatctgcatc ttctccctcc ttggaaaatt tgaagagatt
    10801 tcctaaaagt tgaaaattta ctggtttttg tggcatttcg agctcaaaaa tcctgaaagt
    10861 tttaactttt taggttgtcg aatagccgta taagcggcgt atgagacaat ctcttcaccc
    10921 agttatgggc acacgcttct attcatgtgc ccagagcgac ggcgccgcgt gggcacctga
    10981 atccttttcc acaggaaggg gagttctttt tcaaacggta tgttttgccc gcagcataac
    11041 ttaccttaat cgaaaataac ggtataaagt gttattaact atacaaaaga gagagcaaat
    11101 ttagcgaagt tcaacaaagt tgacgttagt gagagttcgt taatgacaag tttaatgggg
    11161 ttgataaaaa aattacaatt tttgtaaaaa ttgttgaatt tttggcaaaa aaaggcgttc
    11221 taatgcggcg taaatcgtac gcttgaacgg cacaatctga tttgtcgcct cggccatcgg
    11281 acgaaatgca acccgtgcaa attcttgcgt gtgcgtgtgt aagttggtgt cgtttgtggg
    11341 gtggttccag taggcaaggt accatttttg catttgtcca cggtagcgat cggtcatctg
    11401 ggggataact agtccttcgg ggaaattata gtacagtaat tcgggcgctt caaaaatgaa
    11461 caaggcgtca cgaataccgg tttcttcggc gagctcacgc tgcgctgcca cgacaaactc
    11521 ttcaccggcg tcgacgcctc cttgcgggta ttgccatgca cccgggttgt cgaggcgttc
    11581 gcccacgaga gttaatccct ctttattaaa aacaacaata cctacattag gtctatacgg
    11641 caggggggat ggcataaata ggttacatat acttggccgt gtcggccaag tatatgtatg
    11701 aaacaagctt agtctgcttg gccttcgaga agtacggtta caatgtcgcg tgctttgagg
    11761 ggcatttgag cgagtaagcc ggtagtcacg aggctagcgc tgtcagaaac agcagctaaa
    11821 acttttttac ctgcaatgtc ataaacagaa gcgtagaatt caacgccggt agtttgagga
    11881 acagccatgc cgctaccttt ttctgcgctg acgaatacga ttgtgtcgaa tttaccgagt
    11941 tttgctgaga gtttcagaat ttcatcaatt gctaatgctt tcaggttttg gggatcaaca
    12001 acgcctttgt cttgtaagcc aaagttagta taaattttgg taacaccttt tacagaatcg
    12061 ggtattgtgg gtaatacttt cttggaaccg actttttcag cggctgcacc tttgaatgca
    12121 aggcttactt ttgtgtcgct agaagcgtta tcttcaacaa caacgagtac gcttttgttt
    12181 ttcaaatcaa tttttgtagt tttatcggga aaacgaacgt tgccgcttcc gcatgcgata
    12241 aaggatgctg cgacgacgca gacagccaag gtcttcaata gtttcataat tctcctaaaa
    12301 ttcttctaaa ttcctgatgt caggtgagta agcggattat caatatactt ggcgtcaata
    12361 aaaatgggtt ttctaagagc ctgtgcgcca actcacgcag gctcctaagt cgtacaatgt
    12421 agaaccccga gacatcggtg acagctatgc agccaatttt gctggtcgct
//
LOCUS       DAOMPD010000014        12408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2198_length_12408_cov_3.014490, whole genome shotgun sequence.
ACCESSION   DAOMPD010000014 DAOMPD010000000
VERSION     DAOMPD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2198_length_12408_cov_3.014490"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..1348)
                     /locus_tag="PLY93_01090"
     CDS             complement(455..1348)
                     /locus_tag="PLY93_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRP68112.1"
                     /translation="MKLAIIGDMHQQFDADDISFFNASDYDALLFVGDLASKNPESVF
                     KLLPHLESLTKPAYLILGNHDTTGIRQLIGEITHADFLVQLGTPAQTERMERLKNTLK
                     HVTLCGYSIHDLGDIRLLAARPFSMGDSRASKVAHPMRLTVNFKPYMQKNYNVATLQD
                     SALKLQSLINAVNPPYLILAHNGPYGLGKKVTDLFGADFLPEETDFGDRDLKAAIDYA
                     HKIGKPPLAVVAGHMHYPTKHGNIMKEWYKVQEDVHYINAARWPRIFRNEGRLVHHHV
                     ALSFEGKNCIVEAREIVYHSM"
     gene            complement(1358..1843)
                     /locus_tag="PLY93_01095"
     CDS             complement(1358..1843)
                     /locus_tag="PLY93_01095"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRP68113.1"
                     /translation="MVENIYAFNARRLNGEEIKLSEYKGKALLIVNTASKCGFTPQYD
                     GLEALYEKYSDKGFVVLGFPCNQFGSQEPGSSQDIESFCRLTYSVKFPMFEKIDVNGD
                     AAHPLYKYLTSEKPGVLGLEAIKWNFTKFLIDKKGNPVKRYAPTTKPEDIAGDIEKLL
                     A"
     gene            complement(1979..5113)
                     /locus_tag="PLY93_01100"
     CDS             complement(1979..5113)
                     /locus_tag="PLY93_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRP68114.1"
                     /translation="MIRIVDFSLKHRGWVLAIFVLIFIGGFVALKNLPIDAVPDITNV
                     QVIVNARTGGLDPQQVEKSVTFFIEAEMAGIPNVQEVRSLSRFGLSQTIIIFEDGTNI
                     YWARQQVAERIQSATEKIPQGVSVEMAPITTGLGEVFMYVLQLKEDSPHAKEGYNERL
                     VYLRTVQDFILKPYLKAHVKGAAEVDSLGGYEKQIDLDVDPEALERYGVTLNNIVQKF
                     KSLGENYGGGYIEEKKQQIIVRTEGGYSLNHLGEMPIKMSYDGKPIRLKKIAQVRTYH
                     PQRLGAATYRGEETVLGTVLMLMGQNSRDVAKSAANALKSAPLPNDIETQVVYSRSFL
                     VDATIKTVIKNLIEGAALVIIIILLFLNNTRAAFFVMLSIPFAMVFTLIGMKNLGVSA
                     NLMSLGALDFGLIVDGSIVMIENVITLMEKHKPKTNAEKLQLVKNSAREVIGPLATGL
                     IIIMGVYLPILTLEGVEGKLYYPMAITVLFALLGALVMALFVIPTLACFVNVKPSKNK
                     SAVMEKLYRAYEPLLNFSIRHTRKTLVSTVAFLAFCIFAFSRLGSDFMPPLNEGDITF
                     NFLHDAKISLSETLHREKAAEKKLLTYPEVDTVFSRIGTSEAATDPMGVNLTDLFVIL
                     KKDQSQWRKNARGKTISYAELIEKMRNDLQPLLKEEWNLAESELVETQPIAMRFNEML
                     EGSRADISLRIYGKDLDTLIDLQTRIIAILKQIRGASEIELDALTALRKSRILDARLK
                     RDRLNFYGIALQDANEALEMAMVGRNVGSYYEEDWRFRVDVIIADEKRKDLNLIKKIP
                     LALPEGGNIRFGDIAEFKYSENVTSIARSSLTRYAGIAIYLKDRDTLSFVDEAKTRIA
                     QEIQLPPMVHLGWGGQFRNLERAQKKLIVIVPLALLAIAFLIYRTFRSINQTFLIFLT
                     IPFAWTGGILALWISGLSFSVSAAVGFIALSGVAVLNGLVEVSYINQLIADGHPVADA
                     VHLGARGRLRPVLMTALVAGLGFLPMVLNTGIGAEVQRPLAVVVLGGLITATLMTIFL
                     LPALYNLLERKKSV"
     gene            complement(5188..5577)
                     /locus_tag="PLY93_01105"
     CDS             complement(5188..5577)
                     /locus_tag="PLY93_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68115.1"
                     /translation="MRLLASLNLFYLFFLAVSVFSSLHVDHPGSHAQICVANKITAEC
                     HSEMPHSHEKSHPGEDSLLEHAFEDGASLDKAFLPLYAIEHTIGKIVTDTTVITEPLL
                     EATTARWYSALDTPPISRGPPTPSKKA"
     gene            complement(5583..6278)
                     /locus_tag="PLY93_01110"
     CDS             complement(5583..6278)
                     /locus_tag="PLY93_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68116.1"
                     /translation="MLDTELTENNLTEIESGKRLFVKSYGLARTTDGFLRTAIKRIMH
                     RLKRPELTQAVEVILRELTMNAAKANFKKIFFAEKGFNSNDPETYALGMAEFRETMSE
                     DMFATYGQKAREADLNILITLDYDENRIILEVRNNVPMSINEEKRARDKLRTAMQATD
                     LAQLFVDNIDESEGAGLGLMLCLTTLRSSDIDPRMFSVATDFKKETVARVEFPLNKEY
                     VPARKLWQEKMAV"
     gene            6430..7713
                     /locus_tag="PLY93_01115"
     CDS             6430..7713
                     /locus_tag="PLY93_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HRP68117.1"
                     /translation="MLRLVLCLLSVLCISPSLYAWSNHFLFYHAALEVLPEVANTPKI
                     KVESLEDFLLKEQKGLVEEMAKMENEVKTTYAKYAPQPEALAFKGGDKKTIRENFLRA
                     LRVNPKTPFGFFLQELPGNKLPGAAANSLSASMFGDKDIKDKYALYDIRNGQSVDALE
                     VLATAGDEPDFGMDINLFVDNEGPLGKDYGFGTQSFGNPKLYFGTQAPFHIGYYHESW
                     IIFKAGSFLKRTYPKYRVHQYLTLARFAFKTGHPYWGYRFLGWGLHYIGDLTQPYHAR
                     VLPNNSTLRMLWINIKAMLGFEASKIAAIEDVSDRHLTLERYHFSVLRDAYVKHNLNH
                     PFIQSVKVTQKDAGYGAYNADYIENVLTQESYGISDELDTLIDKSNLLKGFVDGKVTA
                     EVNAPALKELDALIATHMQGIGAHVRNYVRAGIMK"
     gene            complement(7747..8439)
                     /locus_tag="PLY93_01120"
     CDS             complement(7747..8439)
                     /locus_tag="PLY93_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRP68118.1"
                     /translation="MDNKNTVSRKNQTNPPVNAAKPIRFTRRPALFFVGFATIALSAA
                     LFAAYPKWHKQDTENWVYSYVTDRFPGKGVENLQRLAQIIYRESNKLRLDWASDGAKY
                     DRAALLLGVIQLESEFNRKARSKVGARGFMQLMPATAALLKGKRLTNEELIDAETNIT
                     LGVRYLNDMIREMGSVEKALLAYNAGPGAVRRWGGVPAYYTQVVKGHNQFLAYRDEKS
                     NARMLVITQLIE"
     gene            8737..>12408
                     /locus_tag="PLY93_01125"
     CDS             8737..>12408
                     /locus_tag="PLY93_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRP68119.1"
                     /translation="MRRIYSIIILALVTMFFSCRTNPFGDTEKSFEEFNLYTTDFTAQ
                     TDGGAKVALLNVIQSSRATLHCVFSKLTLTDVTDALIARASAGVQVKVAFDRNIKDTD
                     AGSIALQASAKFTVITTPSDGNESQLLYGNANTNAFVRHNFCMADERFIYISTAAPDD
                     NQMRKMPNVALKIGSPQFGLARDFLREANMFGQLLFGNGKSKTDFTTKFTALDQVIGV
                     YWGPQESALDVLGTELSEANKSVDFYSTSFLATNTSATKADRDVPTTLKGLETTKGLP
                     LKKYFSSAALFAAGSQAYTLSNPHIYTGAAVVGANIFVVDRGAINAKTYIYTGALRKE
                     GNSSDDSVLIVLKGRRIADAMGAYLDSIGTASTIVTNTGDTSAAGEVVVSELMWMGSY
                     SNDLVSDSADEFIEFYNTTSSPINMSGWKFACTKDGITANSAITLPPGAIIGSGGYFV
                     VAKKDSGAFGPAVNHIDTKVGITDDSYQCKLGNGKTSAGVYGITDFGSVTLDTVGDGS
                     TKFNAAGWDRGSNKGSPTYIKRSMERIDTTVGSGVINGAWQTNVFEPSQNIYVVDNYR
                     TQTFATPGAAASNYLPSVAFERGVFFTTDNTKVNGRAKITVLSPSANTNAGVAEGVTV
                     TVTSTSDSTGISLTLTETGVNTGLFETGTLLNFTTSASVSNEIKVVSGDTITATYTFN
                     TVTYTATALWFPLVPVINEIGVSPGSNDFVEIFNPNTQAIDLAATKLYLQRDSACNLS
                     NGVTENLALSGTIPAGGYFTLAKSGHTLPNIDAATIGNIDVGYCVILSATNKETTAST
                     DNTIVDWVTIAGTGDAENSSYAPTTTTDGAISRIPNGADTDMNANDFKLKPATPGTIN
                     GTPTFTSTPTNGAAGVLTNANVVLTFNEAMNNGMGVLTLAGTSSGTQANQPCVWSVGD
                     TVCTVTPASAFQTNETVTVTLNNFKNTSLNLAPLVPGFNFTTVNPALTPTVTNVVVTS
                     TNPNNGTTPFNTGATTLTITGTNFTGTTAVNLDDLNGANVAVNTPLTSVTIDSATQIT
                     ATVPAGVRTNASTGWNVRVTNASGTNATSSVKFVPQAGLLISEVVQNAGAAANEFIEL
                     YNTTGLAIDIGPTGLNLRLHTRNSSGVDTNKTLTATGASCAINDTTIPAHGFYLIVST
                     AAFAQTWWPKRNMTYSAGITQDGAVYISLSSTTDLLVIDKVGWGTVPPLNYEGTVVSA
                     LGANQSIERKPAGDLGHSTD"
BASE COUNT         3301 a         2880 c         2907 g         3320 t
ORIGIN      
        1 atatatagag ggcaaatatg ttaaagacat cgattagttt acccaaagac tccgtcacaa
       61 cgtggcgcca aaaccactgg gagatcatgc gcatgtatgc acgattctta cgggttgtcg
      121 agcgcaagag aaagtttcgg cggaaggtga cgcggcaata caactggggg aatgggcatt
      181 gtgcgattgt gaaggtgcga ttcacagcga aggagtatga ttcgctgcat acaatggcag
      241 cgggaatacg ggtgagtgta tcgtatctta tttacgatct cattcaattt tgggaaaaag
      301 cgtcgaaaaa atgtgccaga ttcattgttg ggggtaacta tgcgtcccga gtgctggagt
      361 ggtcgaaaag gagggtgaaa tttgaggaaa aactctcttt caggtgtgta ggctcccgtt
      421 ttttcacccc actaagacca tagcacatct tatactacat ggaatggtac acaatctctc
      481 gcgcttcaac aatacaattt ttcccttcaa acgatagcgc aacatgatga tgaactagac
      541 gcccttcgtt acgaaaaatg cgtggccaac gcgccgcatt aatgtaatgt acgtcttctt
      601 gtactttata ccattctttc attatattgc cgtgtttagt ggggtaatgc atatgtcctg
      661 ccactaccgc taatggtggt ttgccgattt tgtgcgcgta atcaatagca gcttttaaat
      721 cgcggtcacc gaaatctgtt tcttcgggca ggaaatctgc gccaaataaa tcggtgactt
      781 ttttacctaa accatacggc ccgttgtgcg ctaaaatgag ataaggcgga ttgacagcgt
      841 tgatgagcga ttgtagttta agcgcgctat cttgtagagt cgccacattg tagttttttt
      901 gcatatatgg tttaaaattc accgtaagac gcatcggatg cgcaactttc gatgcacggc
      961 tatcgcccat cgaaaaaggg cgcgccgcta aaagtcgtat atcccccaga tcgtggatgg
     1021 aatagccgca cagtgtgacg tgcttcaacg tatttttcaa ccgctccatc cgttcggttt
     1081 gtgcaggcgt tcccagctgt actagaaaat cggcatgcgt aatctcaccg ataagttgtc
     1141 gtatacctgt ggtatcgtga ttccccaaga taaggtatgc gggtttggtt aaactttcta
     1201 aatgaggtag taatttaaaa acactctcag ggtttttaga ggccaagtca ccgacaaaaa
     1261 gcagcgcgtc gtaatcggac gcattaaaaa acgaaatgtc atcagcatcg aattgctgat
     1321 gcatgtcgcc aatgatggca agcttcatta aagactttta tgctaaaagc ttttcaatgt
     1381 ctccggcaat atcctcgggc ttggtcgttg gtgcatatct tttaactgga tttccttttt
     1441 tgtcgatgag aaattttgta aaattccatt taattgcttc gagccccagc acccccggct
     1501 tttccgaggt gagatatttg tagagcgggt gtgccgcgtc gccgttgacg tcaatttttt
     1561 caaacatggg aaatttcacc gaatatgtga ggcggcaaaa tgattctatg tcttgtgagc
     1621 taccgggctc ttgcgaacca aattgattac acgggaaacc caaaaccaca aaacctttgt
     1681 cgctgtattt ttcgtacaac gcttcaagcc cgtcgtattg tggggtgaat ccacatttgc
     1741 tggcggtatt cacaatcaag agcgctttac ctttatactc gctcaatttt atttcttcgc
     1801 cgttcaagcg gcgcgcgtta aacgcataaa tattttctac cataattaac ggcgttggat
     1861 ttcatcgtgc ggtgtaaagt agttaggagc ctgtgagaca gtgcgccaac tcatgcagtg
     1921 tgtacaaaaa tttgcaaaaa aattgcctca gcgaattggt gcacaggctc ttaggtggtt
     1981 aaactgattt ttttctctcc aacaaattgt agagtgcggg taaaagaaaa atagtcatga
     2041 gtgttgccgt gataagccca cccaaaacaa ccaccgcgag tggacgctgt acttctgcgc
     2101 cgatgccggt attgaggacc atcggtaaaa atcccagtcc tgccacgagc gcagtcatga
     2161 gcacggggcg taatcgtcct cttgctccca aatgcaccgc gtcggcaaca gggtgcccat
     2221 cagcgatgag ttgattgata taactgacct cgacaagacc gttcaaaact gccacccccg
     2281 atagcgcgat aaaaccgacg gcggcagaaa ccgaaaacga aaggccactt atccacaacg
     2341 caagaatgcc gcctgtccat gcaaaaggga ttgttaaaaa aattagaaat gtttgattta
     2401 tcgaacggaa tgttcgataa atcaaaaacg caatcgcgag gagcgccaac ggtacaatca
     2461 cgatgagttt tttttgtgca cgctctagat tacgaaattg accgccccat ccaagatgaa
     2521 ccatcggcgg cagttgaatt tcttgagcga tacgcgtttt tgcttcatcc acaaacgaga
     2581 gagtgtcgcg gtccttcaaa tagattgcga tgcctgcgta gcgcgtcaat gaactgcgcg
     2641 caatcgacgt cacattttcg ctatacttaa actcggcaat gtcgccgaaa cgaatgttcc
     2701 ctccttcggg taacgcaagt ggtattttct tgataagatt taagtcttta cgtttctcgt
     2761 cggcgatgat gacatcgaca cggaaacgcc aatcttcttc gtagtagctc ccaacgttgc
     2821 gccccaccat cgccatttcg agtgcttcgt tcgcgtcttg taaagcgatg ccataaaaat
     2881 tgaggcggtc gcgttttaag cgtgcgtcta aaatacggct cttgcgcaac gcagtgagcg
     2941 catcgagttc aatttcgctt gctccacgga tttgtttaag aatcgcaatg atgcgcgttt
     3001 gtaaatcgat gagtgtgtct aaatctttgc cgtagatacg caatgaaata tcggcacgac
     3061 taccttcgag catttcatta aagcgcatcg caatcggttg tgtttccaca agttcggatt
     3121 cagccagatt ccattcttcc tttaagagag gttgtaaatc gttacgcatt ttttctatca
     3181 actcggcgta acttattgtt ttaccgcgcg cattttttcg ccattgggat tgatcttttt
     3241 tcaaaattac aaacaggtcg gttaagttga cacccatggg gtcggtcgca gcttcgcttg
     3301 tgccgatgcg gctaaacaca gtatcgacct ctgggtaggt cagtaatttt ttctctgctg
     3361 ctttttcgcg gtggagagtt tcgctgagcg aaatttttgc atcgtgtaaa aagttaaacg
     3421 taatatcgcc ctcgttcaac ggtggcataa agtcgctccc taaacgcgaa aaagcaaaaa
     3481 tgcagaatgc gagaaacgcc acagtgctca caagagtttt gcgcgtatgt cttattgaaa
     3541 aattaagcag tggttcataa gcacggtaga gtttttccat aacagcgctt ttattttttg
     3601 aaggttttac gttcacaaag caagcaagcg tcggtataac aaaaagcgcc atcacgagcg
     3661 ctcctaaaag tgcaaagagc acggtaattg ccatggggta atagagttta ccctcgacac
     3721 cttcaagcgt caaaattggt aaataaaccc ccatgatgat gataaggccg gtcgccaacg
     3781 ggccgataac ctcccgcgct gaatttttta ccagttgcaa tttctcggcg tttgttttgg
     3841 gcttatgctt ttccataagt gtgataacat tttcaatcat gacaatcgag ccgtcgacga
     3901 tgaggccaaa gtcgagtgcg cctaaactca ttaggttagc ggaaactccc aaatttttca
     3961 tacctatgag cgtgaacacc attgcaaatg gaatcgacaa cattacaaag aacgctgcgc
     4021 gcgtgttgtt caaaaaaaga aggatgatga taatgacaag cgctgcgcct tcgattaaat
     4081 tctttataac ggtttttatc gtcgcatcca ctaagaagct ccgtgaatac acaacctgcg
     4141 tttcgatgtc gttggggagg ggagcgctct ttagcgcatt ggcggcgctt tttgcaacgt
     4201 ctcggctatt ttgccccatg agcatgagca ctgtgcccaa caccgtctct tcgccacggt
     4261 acgtcgctgc gcccaaacgt tgagggtggt atgtccgcac ttgcgcaatt tttttcagcc
     4321 gaataggttt accatcatag gacattttaa tcggcatttc gcctaaatgg tttaaagagt
     4381 atccgccttc tgtacgcacg atgatttgct gttttttttc ttcgatatat ccaccgccgt
     4441 aattctcgcc gagcgactta aatttttgaa caatgttgtt gagagtaact ccataccttt
     4501 ctaacgcttc ggggtcgaca tctaaatcga tttgtttttc ataccccccg agggaatcca
     4561 cttcggcggc gccttttaca tgcgctttta agtatggttt caaaataaag tcttgtacag
     4621 ttcgcaaata gaccaaacgt tcattgtagc cttcctttgc gtgtgggcta tcttctttaa
     4681 gttgcaagac atacataaaa acttcaccca agccggttgt gatgggcgcc atctcgacgc
     4741 ttacgccttg tggaattttt tctgtagcgc tttgtatacg ctcagcgact tgctgccgtg
     4801 cccaataaat attcgttccg tcttcaaaaa taatgatagt ctgcgaaagt ccaaaacgcg
     4861 aaagcgagcg gacctcttgt acgttgggga tgcccgccat ctcggcttcg ataaagaacg
     4921 tgaccgattt ttcgacttgt tgtggatcga ggccaccggt gcgtgcgttc acgattacct
     4981 gtacgttggt gatgtcgggc accgcatcga taggtaaatt ttttagagcg acgaatccac
     5041 cgatgaaaat aagcacaaaa atagcgagca cccaacctcg gtgctttaag gagaaatcaa
     5101 caatacgaat cataataccc tccgattgaa agccttgtgc gcagcttata gaaaacgcac
     5161 aggttttatt tttaagcgat gaatcgctta cgcttttttt gaaggtgtgg gtggaccgcg
     5221 tgagatgggt ggggtgtcga gagccgagta ccaccttgca gtggtggctt ccagaagggg
     5281 ttcggtaata accgtggtgt cggtaacgat tttacctatc gtgtgctcga tagcataaag
     5341 gggtaaaaaa gctttgtcta acgatgcgcc gtcttcaaag gcgtgttcaa gaagtgagtc
     5401 ctcacccggg tgcgatttct cgtgcgagtg aggcatctca ctgtggcatt ccgccgtaat
     5461 tttattggcg acacaaattt gtgcatggct accgggatga tcaacgtgta gcgaagagaa
     5521 aacagagacc gctaaaaaga aaaggtagaa taaattgagt gaagccagta agcgcacgta
     5581 aattatactg ccattttttc ttgccagagc ttccgtgctg gcacatattc tttattcagc
     5641 gggaattcga cacgcgccac tgtttctttt ttgaagtcgg tggcgacaga aaacatgcga
     5701 ggatctatat cggaagagcg taatgttgtc aaacaaagca tgagtcctaa gccagccccc
     5761 tcgctctcgt cgatgttgtc gacgaaaagc tgcgctaaat cggtggcttg catcgctgtg
     5821 cgtaatttat cgcgggcacg tttttcttca ttgatggaca tcggtacgtt gttacgcacc
     5881 tcgaggatga ttcgattctc gtcgtagtct aaagtaatga ggatgttgag atcggcctcg
     5941 cgcgcttttt gaccgtaagt ggcgaacata tcctcagaca tcgtctctcg aaactcggcc
     6001 ataccaagag cgtatgtttc gggatcgttg ctgttaaagc ctttttcggc aaaaaatatt
     6061 ttcttgaaat ttgcctttgc tgcgttcatg gtcagttcgc gcaaaataac ttcaaccgct
     6121 tgcgtcagct ctggcctttt gaggcggtgc ataattcgtt tgatggcggt tcgcaaaaaa
     6181 ccatcggtcg tgcgtgcgag accgtatgat ttaacgaaga ggcgtttacc cgattcaatt
     6241 tctgtcaggt tgttttcagt gagttcagtg tccaacatga gatttatctt tatctatgta
     6301 gtaattgcta tacaagcgct ttgatgccaa aaattatgca acctacagag gagcctgtgg
     6361 ctattttttc tctcgaagga aagaatttag agttgacgca cagactctca ggacttcttg
     6421 tagtgtgata tgctacgttt agttttatgc cttttgagcg ttctgtgtat atccccttct
     6481 ctttacgcat ggtcaaacca ctttttattc taccacgccg ctttagaagt gttgcccgag
     6541 gtggcgaata ctcccaaaat taaagttgag tctttggagg atttcctcct aaaagagcaa
     6601 aaaggtcttg tggaagaaat ggccaaaatg gaaaacgagg tcaagacaac gtacgctaag
     6661 tatgcaccac aacccgaagc gttagcattc aaaggtggcg ataaaaaaac aatccgtgaa
     6721 aactttttac gcgctttgag ggtaaatccc aaaaccccgt ttggtttttt tctacaagag
     6781 ttgcccggta acaaactacc cggtgctgcc gccaattcgc tttcggcaag tatgtttggc
     6841 gacaaagata tcaaagataa gtacgcgctc tacgacatcc gcaacggcca aagcgtcgat
     6901 gcgctcgaag ttttagccac cgctggcgat gaacccgatt ttggtatgga tatcaacctc
     6961 tttgtcgaca atgaaggccc cttgggtaaa gactatggct ttggtactca atcgtttgga
     7021 aatcccaagc tttactttgg aacacaggcg ccttttcaca tcggctatta ccatgaatcg
     7081 tggattattt tcaaagcggg ttcgtttttg aaacgcactt atcccaagta tcgtgtgcac
     7141 cagtacttga ctctcgcacg ctttgctttc aaaacaggcc acccctattg ggggtatcgt
     7201 tttttagggt gggggctgca ttacatcggt gacttaacgc aaccgtacca cgcacgggtt
     7261 ttgcccaaca acagtacgtt acgcatgctg tggattaaca tcaaagcgat gctaggtttt
     7321 gaagcgtcga agatcgccgc catcgaagat gtctccgacc gacacttgac gttggagcga
     7381 taccattttt ccgttttacg cgacgcgtat gtaaagcata atctcaatca tccttttata
     7441 caaagcgtta aagtaaccca aaaggacgcg ggatacggcg cttacaatgc agattacatc
     7501 gagaatgttt tgacccaaga aagttatggc atctctgatg aactggacac gcttatcgat
     7561 aaaagcaatt tacttaaagg ctttgttgac ggcaaagtga ccgcagaagt taacgcacct
     7621 gcactcaagg aactcgacgc gctcatcgct acacacatgc aaggcattgg cgcgcacgtt
     7681 cgtaactacg tccgcgccgg tataatgaaa tagcaacgag ataattaaag aacgattaca
     7741 cagcctttac tcgataagct gtgtaatcac cagcatacgc gcgttgcttt tttcatcgcg
     7801 gtatgcgaga aattggttat gccctttcac cacttgtgta tagtacgcgg gcactcctcc
     7861 ccaacgacgc acagcgccag ggcctgcgtt gtacgccaag agcgcttttt caacgctccc
     7921 catttcgcgg atcatgtcgt tcaagtagcg tactcctaac gtgatattcg tttcggcatc
     7981 gataagttct tcgtttgtta aacgtttgcc tttaagaagt gcagccgtgg caggcatgag
     8041 ctgcataaaa ccacgagcgc caactttgga gcgcgctttg cggttaaact cgctttccag
     8101 ttgaatcaca cccaagagta acgccgcccg gtcgtattta gctccatctg atgcccagtc
     8161 aaggcggagc ttattcgatt cacgataaat aatttgtgca aggcgttgca gattttcaac
     8221 ccccttgccg ggaaaacgat cggtgacata cgagtacacc caattttcgg tatcttgttt
     8281 atgccacttg gggtacgcgg cgaaaagcgc agctgaaagc gcaatggtgg caaaacctac
     8341 aaagaagagc gctggtctac gcgtgaagcg tattggcttt gcagcgttta cgggtggatt
     8401 agtttgattc ttacgggaaa cagtattttt gttgtccatg aaatcctcct aagtcttgtg
     8461 caatgcacta tgtgcaatgc acaaacccga gagaggctgt caactcgaat agggataact
     8521 atgccgtgag gccgacggca aattgttcca atttgctctg tatggtgcga agacaccgaa
     8581 tttgtgaaaa tttagcgctt ttgagccaat cgcatataaa atttgcaaaa aaattgctcc
     8641 ttttaaatac gctgcgcggg ttggcgcgca atatctgtgg ctgtccgttt ctgattaaat
     8701 tcttgcactg ttgagtctct caggccatat agtagtatgc ggcggattta ttctattatt
     8761 attcttgccc ttgtgacgat gtttttctca tgccgcacaa atccttttgg cgacactgaa
     8821 aaatcgtttg aagagtttaa tttatacacc accgatttta ctgcacaaac cgacggcgga
     8881 gccaaagtag ccctcctcaa cgtcatccaa agttcacgcg cgacgctcca ttgtgtgttt
     8941 tctaaattaa cgctcaccga tgtcaccgac gctctcattg caagggcaag tgcgggtgtg
     9001 caagtaaagg ttgccttcga tagaaacatt aaagacaccg acgcagggtc tatcgcgctg
     9061 caagcgagcg caaaatttac agttattaca actccctccg atggcaacga aagccagctc
     9121 ttgtacggca atgcaaacac gaatgcattc gtgcggcaca atttttgtat ggcggatgaa
     9181 cgctttattt atatttccac cgctgcgccc gatgacaacc aaatgcgcaa aatgccaaac
     9241 gtggcgctca aaattggtag tccgcaattt gggttggcgc gcgacttttt gcgcgaggca
     9301 aatatgtttg gccaactttt gttcggcaac ggtaaatcta aaacagactt taccactaag
     9361 ttcaccgcac tcgatcaagt catcggcgtt tattggggcc cgcaagagtc ggcgttggat
     9421 gtcttaggta ccgaactttc tgaagcaaac aaaagcgttg atttttattc gacttctttc
     9481 ttggcgacaa acacgagtgc aacaaaagca gacagagatg tgccaacgac tttgaaaggc
     9541 cttgagacaa caaagggctt gcctctcaaa aaatattttt ctagcgctgc cctctttgcg
     9601 gcgggttcgc aagcgtacac gttatccaac ccacacatct acacgggcgc cgctgtcgtt
     9661 ggtgcaaata tttttgtcgt cgatagaggc gcgataaacg caaaaacgta catttacaca
     9721 ggcgcgctac gcaaagaagg aaactcctcg gatgacagcg ttctcattgt acttaagggc
     9781 cgtcgtatcg ccgatgcaat gggcgcgtac ctcgacagca ttggcaccgc ttcgacgatt
     9841 gtcaccaaca ccggcgacac gtccgcagcg ggtgaagtgg tggtgagtga actcatgtgg
     9901 atggggagtt actcgaacga tttagtcagc gactccgccg atgaatttat tgaattttac
     9961 aatacaacga gtagccccat caatatgagc ggctggaaat ttgcgtgtac gaaagatggt
    10021 ataactgcca actcggcgat tacgctgcct cccggcgcta ttataggctc cggcggttat
    10081 ttcgtcgtgg ctaaaaaaga ttcaggtgcg tttggcccag cagtaaacca catcgacacg
    10141 aaagtcggta tcaccgacga ctcataccaa tgcaaattgg gcaacggcaa aacatcggcg
    10201 ggggtttatg gaattacgga ttttgggagt gtgactctcg acacggtggg agatggttcc
    10261 accaagttta acgccgctgg ttgggatcga ggctccaata agggaagtcc cacttatatt
    10321 aaacgcagca tggagaggat tgacaccacc gtgggttcgg gagtaataaa tggcgcatgg
    10381 cagacgaatg tttttgaacc gtcacaaaat atctatgtcg tcgataacta ccgtacgcaa
    10441 acatttgcaa cgcccggcgc agcggcgtcg aactatttac caagcgttgc ttttgaacgg
    10501 ggtgtttttt tcaccacaga caacaccaaa gtcaatggac gcgctaaaat taccgtacta
    10561 agccccagtg caaacacaaa cgctggtgtt gctgagggcg tgacagtcac cgttacctca
    10621 acgagcgaca gtactggcat tagcctaaca ctcaccgaga caggagtcaa caccggtctt
    10681 tttgagacgg gaacactctt gaattttaca accagcgcca gtgtaagtaa tgaaatcaaa
    10741 gttgtttcgg gcgacaccat caccgcaacg tacacattca acacagtaac atatactgcc
    10801 acagcgctct ggtttccttt ggtaccggtt atcaatgaaa tcggtgtgtc gccagggtcg
    10861 aacgattttg tcgaaatttt taatccaaac acgcaagcga ttgatcttgc tgctacaaaa
    10921 ctctatttgc agcgcgattc agcgtgcaac ctttcaaacg gcgttacaga aaatctcgca
    10981 ctctcgggta caataccagc aggaggatat tttactcttg ccaagagcgg tcacacttta
    11041 cctaatatcg atgcggcaac gataggaaat attgatgtcg gttattgtgt tattttgagt
    11101 gcaacaaata aagaaacgac agcaagtacc gacaacacta ttgtcgattg ggtgacgatt
    11161 gccggcactg gcgatgcaga aaatagttcg tatgcaccaa cgacaactac agacggcgct
    11221 attagtcgca tacctaacgg cgctgacacc gacatgaacg cgaacgactt taaattaaaa
    11281 cctgcaaccc ctggaactat aaacggtact cccactttta cttcgacacc cacaaacggg
    11341 gcagcgggcg tgctcactaa cgcgaatgtt gtgctcacgt ttaatgaagc aatgaacaac
    11401 ggtatgggag ttctcacgct cgctggtacc agttccggta cacaagctaa tcagccttgc
    11461 gtgtggtcag tcggcgacac ggtgtgtacg gttacacccg cgagcgcttt tcaaacgaat
    11521 gaaacggtaa cagtgacttt aaataatttt aaaaatacca gcttgaatct tgcgccactt
    11581 gttccgggtt ttaattttac cacggttaac ccagcgctca cgcccacggt tacaaatgtc
    11641 gtcgttacga gcaccaaccc caataacggc acgacgcctt ttaacacggg cgcgacaaca
    11701 ctcaccatta ccggcactaa ttttacggga acaaccgccg tcaatttaga cgacctgaac
    11761 ggcgcgaacg ttgcagtcaa tactccatta acttccgtta ctatagattc cgcaacacaa
    11821 attactgcga cagtacctgc gggcgtgcgc acgaatgctt caacaggttg gaatgtgcgt
    11881 gtcacaaacg caagtggtac aaacgcaacg agtagcgtga agtttgtacc tcaagcgggg
    11941 cttttaatta gtgaggttgt tcaaaatgcc ggcgcagcag ccaatgaatt tattgaactt
    12001 tataatacaa caggtttggc cattgatatt ggaccaacgg gactaaatct acgattgcat
    12061 acccgtaata gcagcggcgt agacactaat aaaacgctta ctgctaccgg agctagttgt
    12121 gcgataaatg acacgacaat tccagctcat ggattttatt tgattgtatc gactgcggca
    12181 ttcgctcaaa catggtggcc aaaacgtaat atgacatatt ccgcgggaat tactcaagat
    12241 ggtgctgttt atattagttt gagtagtaca acggatttgc tcgttattga taaagtcggt
    12301 tggggtacag tacccccact taactatgaa ggtactgttg taagtgcatt gggcgcgaat
    12361 caaagtattg agcgtaaacc tgccggcgat ttaggtcaca gtactgat
//
LOCUS       DAOMPD010000015        12236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2238_length_12236_cov_2.880634, whole genome shotgun sequence.
ACCESSION   DAOMPD010000015 DAOMPD010000000
VERSION     DAOMPD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_12236_cov_2.880634"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..307)
                     /locus_tag="PLY93_01130"
                     /pseudo
     CDS             complement(1..307)
                     /locus_tag="PLY93_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669475.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(311..1597)
                     /locus_tag="PLY93_01135"
     CDS             complement(311..1597)
                     /locus_tag="PLY93_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68120.1"
                     /translation="MEVGIAILLFVCMTALLYVLLSLKITKQVDLQIKEFYKTRIHQD
                     MQEFYREMESYAALFENRIQRFRTLVDKGDSLLGRLNNADAVEKSAGADEIKKVEKEA
                     PPVTAKEIKHTQKKGVVKSQARGAVNVAQKVKKNNAKPVQKANGKAITATIKTPKANT
                     LKLVPSVPVQEKLTVPPIAELPQQQFAEEYDITIAQELLKDIDSVESTHEQSIQKKSI
                     EVKPKEVSVEKAMTQSDATAPESESSIMEIFAQIGRKAKPLFFSEKEKTKLAALTPNA
                     QPARVANTNATPKTPQSGNQAAVSDFTEVLRRAEEIKAQKKAEREKAELDARATFYAQ
                     GDSFANATKFEKEQETNVRPVTTQENETQRNTLAVKKLDQHTINFLIDSLVKDNGYRK
                     QALRALTENNVPLSEIAWLSKIDIGELELMRQLGRF"
     gene            complement(1662..2105)
                     /locus_tag="PLY93_01140"
     CDS             complement(1662..2105)
                     /locus_tag="PLY93_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68121.1"
                     /translation="MRWAFYLSVLLFFLSCGTDIPFDQELYLKRPTGLSVTAIADKKM
                     SVTYYVQNQEATFDGYNLLIDRLTIGDGNAQTMEPLTIDGGVPTFQHSPSDYNINTPR
                     TAIISRFTNVLPFEVGTTYFFRLQAHSRKGVRSEASNEVSATAFQ"
     gene            2204..3229
                     /gene="lipB"
                     /locus_tag="PLY93_01145"
     CDS             2204..3229
                     /gene="lipB"
                     /locus_tag="PLY93_01145"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRP68122.1"
                     /translation="MQSTYAYWGLTPYPDAIARLEEHFEKALATPHAFVFGLEHPLVY
                     TAGLRTEKNHIVNPNIEIQTARRGGSVTLHNPGQLVVYFAMSFHETPGLETLVRLLEG
                     AIIETLWSYGVQAFIEPPHSGVFTQHGKIGFIGLGLKKQVVYHGIAININNNLSDYQA
                     IHSCGLTLPVTRLKDLIKEEIPLEEFGAKLFQKLENRFSMFDQVVYKTQASVSIDELP
                     FPFAAFRLGQLFFNERRFWLAHETWEHIWQDYRAVYGKENKTEYIMFFHGLIQLAMAA
                     YRAFEVVRVAGAESLLGKSLEKLPQNRYFPIYVKNHTDVLRWMHAAHDGLKKGNIPPF
                     PPPILTF"
     gene            3452..4564
                     /gene="recA"
                     /locus_tag="PLY93_01150"
     CDS             3452..4564
                     /gene="recA"
                     /locus_tag="PLY93_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRP68123.1"
                     /translation="MPMKMSEKTQTAAQKAEAAEKAKALDSVLLQIEKQYGKGAIMKM
                     NGDAMKEIPVISTGALSLDLALGVGGVPRGRIIEIYGPESSGKTTLTLHIVAEAQKLG
                     GMAAFIDAEHALDPSYARKLGVNIDELLVSQPDNGEQALEIAEALVRSNALDVIVIDS
                     VAALVPKSEIEGDMGQATMGVQARLMSQALRKLTGAIHKTNTVVIFINQIRMKIGVMF
                     GSPETTTGGNALKFYATVRMDIRRVESLTKGDEAIGNHVRVKVVKNKVAPPFRKAEFD
                     IYFNQGISREASIIDEGVSLGVLKKSGTWFSYEENRLGQGKENVRQYLIENPKIADEL
                     EAKVRAAHADGSAKKALEEKEKASKSAAKAEAKVEE"
     gene            complement(4569..5156)
                     /locus_tag="PLY93_01155"
     CDS             complement(4569..5156)
                     /locus_tag="PLY93_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRP68124.1"
                     /translation="MRFIDIDTQKPQFDAIKAAAELLADDYPLVFPTDTTFGILMRFT
                     VENAARLHALRRDDLSKPFLAVLSEDFNWKSLVNADFLSHENIHYVETYWPGTNTLLF
                     PKAKHLAYPTGNRIALRMPAVENNRAFHTLVQLCDFALMAPLFNRPGEPVITTRNEAA
                     AKFAEIEYAFWDDFYRPQNPSALWDLSQTPIVKVR"
     gene            5292..6731
                     /locus_tag="PLY93_01160"
     CDS             5292..6731
                     /locus_tag="PLY93_01160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP68125.1"
                     /translation="MNSNTPPKKYFAELGISQTTLQAITDMGFREASPIQEKAIPLLL
                     EGRDLIGQAQTGTGKTAAFAIPIVERVTTEKKLQAMILCPTRELAVQVADEIRKILKY
                     NKDILVATVYGGQKIGAQFRDLKLKPQIIVGTPGRVMDHLRRKTIKMQTIAFFVLDEA
                     DEMLAQGFREDIELILTDAPKERQTILFSATMSKEILNITEHFLTNPAKVDVLAGAHN
                     KPKIDQYFSIVQEKYRVEAAIRLMDFYDIKTALVFCNMRTQVDAITQAFNDRNFTAEG
                     IHGDLNQAQRDKVMARLKSGKVRLLVATDVAGRGIDISHIEAVFNFELPRDMESYIHR
                     VGRTGRAGKSGRAFAFIRATDTGKIKRLEKLHGIQVNPHDIPPVDALTGKRSGSVLEN
                     LKDVAQKANLSKILPYVQTLVEGGLTTTDIAAAAIKILLGSLDGEYDTHAQFISNKEE
                     MSKQNSDKKQHGSFKKGKGPRKFGGKPRR"
     gene            complement(6796..8793)
                     /locus_tag="PLY93_01165"
     CDS             complement(6796..8793)
                     /locus_tag="PLY93_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68126.1"
                     /translation="MCLVGVSPTYAFDYLEHSLFTDRACYAAQRILAENVAAGGVREK
                     SRYLALALVCPQKWQPTYCKSGKKNFDGQMRFNGKHTATLGDYSALVDHITEYGKIKS
                     LPNAKDRGFVDLLWKSFLPDAGTGVAGAIAQKGCDIDEDPHWYEIENDVAAFFGVWDA
                     EKRSLKLDTSLLSEDKRAPLSPGPVDPSTLFTIKNPRFLDLQLFNQNHFGKNAYRTWA
                     GMHYPALQVSAAKCEDTVDLDDSVAEDLAEDLPQFQNIDWDSDDNPTYAEDSCRLLRE
                     VLRRRLVEWHKRGERSLVEPVLPLLSLLENNPTSPEAHQVLMELPSNVAALALEGGGL
                     HYMQDALSGGHIRTEHTGRSLSVTRRYHDIDSKNGVFVAFATRENQFEFVAYGDTYLL
                     GKTERVKGESHLENCEDIPQGLRPSPARVSRCLLGYQRGMLTAVNAASLVDWNLGGIL
                     YRDASVLSKEDSPTGCPEKSSAASFICAYLPLQAFTAPPVVAVHGMGYLGREGLPPTP
                     PPIDFQSLRIESFLDAAGNGTQLGARLEFLTPFTRSAGDWLYSYDFGFLMTLGELKRQ
                     QLKHEFAFTFHYRLATRFLINAGPLTYMGFEGFSSQTNFFFGIGLGGGITILPEGWTK
                     IPLELTLSYRAPWRFIDTEHGFRGQRMEAHWVGLSIGLAFL"
     gene            8950..9210
                     /locus_tag="PLY93_01170"
     CDS             8950..9210
                     /locus_tag="PLY93_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68127.1"
                     /translation="MKKNNLLVIAALTGLAMGGLSAKKPKSKAKFGECHGINACKGQG
                     ECGSKDHACGGMNACKGQGWKKMTKAECKAKKGTFKPLSMDM"
     gene            9218..10036
                     /locus_tag="PLY93_01175"
     CDS             9218..10036
                     /locus_tag="PLY93_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68128.1"
                     /translation="MRYTRIYFALALLAALWVTFTLLPHEGTLYFAARHKLDFSIAWA
                     YQFRDDLLFSQVDKFMLSLLPFYGGKVWLVGLLLALPLVLSLLWNTPRAALYIALLSS
                     LILWFIFFSIANPLYYFKRLEVMGGSSLRDTLFATAPKDKIPLDENCVYVINPTDVRG
                     ISSVEALDKKSIMKQPRLYLLDLKVVEENYALLNLKNEVGTPLFSDAARAYATAEVEY
                     AKKIQKMLFQEKSICLVANFDKMPDIENKPYSRARRILDARKKMKVDTKIIQLL"
     gene            complement(10472..12199)
                     /locus_tag="PLY93_01180"
     CDS             complement(10472..12199)
                     /locus_tag="PLY93_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRP68129.1"
                     /translation="MHNVELILTLVAGFTAALIFGLITHRIGLSPIVGYLIAGIAISP
                     NTPGFVANHDIAEQLAEIGVVLLMFGVGLQFDFKELWSARRIALPVAFLQALAAVILA
                     SVTTHFFAWSWQSGIVFGLAISISSTVVVLRVLVDANDLHTPIGRLSIAWLVMEDIFT
                     VFVIVLLPVVFSSASLEQLEIALSVALIKIIALFVVAFVLGLWVIPRFLTAIAQTGSR
                     ELFTLAVLVLALGIAVGSTFFFGVSMALGAFLAGMVVGRSEFSLRAATDAIPMRDAFA
                     VLFFVSVGMLFDAQSLLKTPFLVLATVAVVIFVKPLGAFIILTLLGYPVRVAIAVSSA
                     LTQIAEFSFIMAVLGAQLKILPGDAANILVAVAIITVTLNPLIYRTAAPLEKWLRRFS
                     FLHRFFHFAARHTTSPHSSSEMLNDGATRHAIVIGYGPVGRTVSRLLAENGFIPIVID
                     LNLNTLHELKAHNMPVIYGDASHIETLKRAGLMNAHILILSASSIRGGENVIHEARQL
                     NPKIKVLARTAYLRDVPDLRAAGADAVFAGEGEVALSITEFILSTFGATAEQIDRERE
                     RVRAKLFFS"
BASE COUNT         3348 a         3009 c         2926 g         2953 t
ORIGIN      
        1 ctcccataag tgcgagtacg atttcaagct cgataaactt ctcgatgagt ccgggcaaat
       61 ttttttcttg ttgcgcgcta tcggtgtggt cttttgaaac gatgtagcgg ctcaaataag
      121 aaccgacgcc catggcaaaa agataaatac caatggttcg cgaaaactac gtgacgctgt
      181 cgcctaaaag atagctcgac accgtgccgg cgataagctc gtaaataagg ccgcacgctg
      241 cgatgagaaa cgcggagaaa agaagtaacg cgctttgaaa ttgcgccgaa cttacttttt
      301 tcaacatcga ttaaaaccgc cctaattgcc gcatgagttc aagttcgcca atatcgattt
      361 tgctcaacca tgctatttca gaaaggggaa cattattctc agtgagtgcg cgcaacgctt
      421 gctttcgata gccattgtct ttcacaagag aatcgatgag gaagtttatg gtatgttgat
      481 ccaatttttt gacggctaat gtatttcgtt gagtttcgtt ttcttgtgtc gttaccggac
      541 ggacattcgt ctcttgttct ttttcaaatt tcgtggcgtt ggcaaaagaa tcaccctgtg
      601 cataaaaagt cgcgcgtgca tcgagttccg ctttttcgcg ctctgctttt ttttgcgctt
      661 taatttcttc ggcacggcgc aacacttcgg taaaatccga aacggctgct tgatttccac
      721 tctgtggagt ttttggtgtc gcattggtat tggctactcg cgctggttgt gcgttgggag
      781 taagcgccgc cagttttgtt ttttctttct ctgaaaagaa cagcggcttt gctttgcgtc
      841 caatttgtgc aaaaatttcc ataatgctcg attcactttc aggcgctgtg gcgtcacttt
      901 gcgtcattgc tttttcgaca ctgacttctt tgggctttac ctctatcgat tttttttgaa
      961 tagactgttc atgcgtcgac tcgacagaat cgatgtcttt caaaagttct tgagcaatcg
     1021 tgatatcgta ttcttctgca aattgttgtt gcggtagttc ggcaatgggc ggcacggtta
     1081 atttttcttg cacaggaaca cttggcacaa gttttagcgt gttcgctttt ggggttttta
     1141 tcgtggccgt aatggcttta ccattcgcct tttgaacagg cttcgcatta ttttttttaa
     1201 ccttttgcgc cacattcact gcgccgcgtg cttgcgattt gacgactccc tttttttgcg
     1261 tgtgttttat ttcttttgct gtaacgggag gagcttcttt ttctactttt tttatttcgt
     1321 ccgcaccggc acttttttct accgcatcag cgttattcaa ccggcccaag agtgaatccc
     1381 ccttgtctac caacgtgcga aaccgttgaa ttcgattttc aaaaagcgcc gcataacttt
     1441 ccatttcacg ataaaactct tgcatgtcct gatggatgcg tgttttatag aattctttaa
     1501 tttgcaaatc gacttgttta gtgattttta acgaaagtag cacgtaaagg agtgccgtca
     1561 tacaaacgaa aagaagaatc gcgataccga cttccatggt aggtgcttat caacgcacgg
     1621 cgggcgtcaa gaattatgtc acattgttag atttgtaaat tttattgaaa cgcggtcgcg
     1681 ctcacttcgt tcgatgcttc agagcgcacc cctttgcgcg agtgcgcttg cagtcgaaaa
     1741 aaataagtag tgcctacttc aaacggcaac acattggtga aacgagaaat aatcgctgtc
     1801 cgtggggtgt taatgttgta gtcagaaggg ctgtgttgaa atgtgggcac tcccccatcg
     1861 atagtcaacg gctccatcgt ttgcgcattg ccgtcgccga tagtcaggcg atcgatgagt
     1921 aaattatatc catcgaacgt ggcttcttgg ttttgtacat aatacgtgac actcattttt
     1981 ttatccgcga tagcggttac ggaaagcccc gtggggcgtt taaggtatag ttcttggtcg
     2041 aaaggaatgt cggtgccaca tgaaagaaaa aaaagtagca cactcagata aaatgcccaa
     2101 cgcacgcgta attttctgca atgaaggtgt atagtcaaac agaagacaaa atttgccgct
     2161 ttgtctaatt cattgtattc taaaaaatag caacaccgcc accatgcaat cgacatacgc
     2221 atactgggga ttgaccccct accccgacgc catcgcgcgg ttagaagagc attttgagaa
     2281 agcactcgcc acaccgcatg catttgtatt tggcttagaa catccgctgg tgtacaccgc
     2341 cggtttgcgt accgaaaaaa accacatcgt gaatcccaat atcgaaatcc aaaccgcccg
     2401 gcgcggcggt tcggtgacac tgcataaccc tgggcaactc gtcgtctatt ttgcaatgtc
     2461 tttccacgaa actcccggct tggaaacgct ggtgcgtctt ctcgaaggcg caatcatcga
     2521 aacgctgtgg agctatggcg tgcaagcgtt tattgaaccg ccgcattcgg gggtgttcac
     2581 ccaacacggt aaaattggtt ttattggatt gggtctcaaa aagcaagtgg tgtatcacgg
     2641 cattgcgatt aacatcaata ataatttatc agactaccaa gccatccaca gttgtggatt
     2701 gactctcccc gtcacacggc tgaaagattt aattaaggaa gaaattccac tggaagaatt
     2761 tggagcgaaa ctttttcaaa aactggaaaa ccgcttttcg atgtttgacc aagtggtgta
     2821 taagacccaa gcgtcggttt ccatcgatga gctaccattt ccttttgcag cgtttcggtt
     2881 gggacaactt ttttttaacg agcgccgctt ttggctcgcg catgaaacgt gggagcacat
     2941 ttggcaagac taccgcgcgg tgtatggtaa agaaaacaaa accgagtata ttatgttttt
     3001 ccatgggctc atccaattgg cgatggccgc ataccgcgcg ttcgaggtgg tacgtgtggc
     3061 gggagccgaa agtctcttag gcaagtcgct cgaaaagtta ccccaaaatc gttattttcc
     3121 tatttatgtc aaaaatcata cggatgtctt gcggtggatg cacgcggcgc acgatggcct
     3181 aaaaaaaggg aatattcccc ccttcccacc accaattctt actttttgag gaaaattttc
     3241 actttttttt caaaaagtga gaaaattcga ctatttcctg gtatttaata tataaaggca
     3301 ccaagaaaca atcgcacaac ctttttatgt gctttgcgca taacaacgat tgtgcatttt
     3361 cttggccaac gagtgacgca acacgacgct cattgagcct caaaattaag gagacataat
     3421 tatggttgac aatgtagaaa aagatggtag catcccaatg aaaatgagcg aaaaaacaca
     3481 aaccgccgcg caaaaggcag aagcggctga aaaagcgaaa gcactcgatt cggtgctact
     3541 acaaatcgaa aaacaatatg gcaaaggcgc catcatgaaa atgaatggcg atgcgatgaa
     3601 agaaatcccc gtgatttcca ccggcgcgct ttcgctcgac ttggcgttgg gcgtgggcgg
     3661 tgtcccccgt gggcgcataa tcgaaattta tggtccagag tcttcgggta aaaccacact
     3721 cacgctgcac atcgtggcag aggcacaaaa attaggcggc atggcagcgt tcatcgacgc
     3781 cgagcacgcg ctcgatccgt cgtatgcacg taagctcggc gtgaacatcg acgagctttt
     3841 agtttcacaa cccgacaacg gcgagcaagc gctcgaaatc gccgaggcgc tcgtacgctc
     3901 gaacgcactc gatgtgattg tcatcgactc agtggcggcg cttgtgccca agtcagaaat
     3961 cgaaggcgac atgggccaag cgacgatggg tgtgcaagcg cgtctcatga gccaagcgtt
     4021 gcgtaaactc acgggggcaa tccacaagac aaacacagtg gttatcttta tcaaccaaat
     4081 tcggatgaaa atcggcgtga tgttcggttc gcccgaaacc accaccggag ggaatgcgct
     4141 caaattttac gccaccgtgc gcatggatat acgccgcgtc gaatcgctca ccaaaggcga
     4201 cgaggccatc ggcaaccacg tccgcgtcaa agtggtgaaa aacaaagtgg cgccaccgtt
     4261 tcgtaaagcc gaatttgata tttatttcaa ccaaggcatt tcgcgtgaag caagtatcat
     4321 cgacgagggc gtaagcttgg gagttctcaa aaaatcgggc acctggtttt cttatgaaga
     4381 aaatcgttta ggccagggca aagaaaatgt gcggcaatat ctcattgaaa atcccaaaat
     4441 cgccgacgag ctcgaggcaa aagtacgcgc tgcgcacgcc gacggctcgg caaaaaaagc
     4501 gttagaggaa aaagagaaag catcaaaaag tgcagccaag gctgaagcga aagtcgaaga
     4561 gtaagcattt accgcacttt cactatcggt gtttgcgata aatcccacaa cgccgatgga
     4621 ttctgtggac ggtagaaatc atcccaaaaa gcgtattcaa tttccgcaaa cttagccgcc
     4681 gcttcgttgc gcgtggttat gaccggttcg cccgggcgat tgaacaacgg cgccatgagg
     4741 gcaaaatcgc ataattgcac cagcgtgtgg aatgcgcggt tgttttctac ggcaggcatg
     4801 cgcagtgcaa tgcgattacc tgtggggtac gcgaggtgtt ttgctttagg aaaaagcaac
     4861 gtgttggtcc ccggccaata cgtttcaaca tagtgaatat tttcgtgcga gagaaaatcc
     4921 gcattgacga gcgatttcca gttaaaatct tcggaaagca cagcaaggaa aggtttagat
     4981 aaatcgtcgc ggcgcagtgc gtgcaggcgc gcggcgtttt caaccgtgaa acgcatcagg
     5041 ataccaaacg tggtatcggt ggggaataca agaggataat cgtcggcgag gagctcggcg
     5101 gcggctttga tggcgtcaaa ctggggtttt tgggtgtcga tatcgatgaa gcgcacacgg
     5161 ctatcatcga cggcgagtca gtgcgccaat cttattccag tgacacgatg tctcactttc
     5221 cggatgtgga aatgaacttg cgcgtcaaac tttacggttg cctctcaagc gcaatatagg
     5281 tcgattcacc tttgaactca aacacccccc ctaagaaata ctttgcagag ttaggtattt
     5341 cacaaaccac tttacaagcg attaccgaca tgggctttcg tgaagcttca cccatccaag
     5401 aaaaagcaat cccccttctt ttagaaggcc gggatctcat cggccaagca caaaccggca
     5461 ccggcaaaac cgctgcattc gcaatcccca ttgtcgagcg cgttaccaca gagaaaaaat
     5521 tacaagcgat gattctctgc cccacgcgcg agctcgcggt gcaagtcgcc gacgagatac
     5581 gcaaaatttt gaaatacaat aaagatattc ttgtcgcgac ggtatacggc gggcaaaaaa
     5641 tcggtgcgca attccgcgac ttaaagctca aaccgcaaat cattgtcggc acccccggac
     5701 gtgtgatgga ccacttgcgt cgcaaaacga ttaaaatgca aaccattgca ttttttgtcc
     5761 tcgacgaagc cgatgaaatg ctcgcgcaag gtttccgcga agatattgaa ctcattttaa
     5821 ccgacgcgcc taaagagcgg caaactattc ttttttcggc gacgatgtcg aaagaaattt
     5881 tgaatattac cgaacatttt ttgacaaacc ccgccaaggt agatgttctc gccggtgcgc
     5941 acaataagcc caaaatcgat caatattttt ctatcgtaca agaaaagtac cgcgtcgaag
     6001 cggccatccg cctgatggat ttttacgaca tcaagactgc cctcgtgttt tgtaatatgc
     6061 gcacccaagt cgatgcaatc acccaagcgt tcaacgaccg caacttcacc gctgaaggta
     6121 tccacggcga cttaaaccaa gcgcagcgcg acaaagtgat ggcgcgcttg aagagcggca
     6181 aagtacggct cttagtcgcc accgacgttg cgggacgcgg tatcgacatc agccacatcg
     6241 aggcagtatt taacttcgag ttaccgcgtg atatggagtc gtatatccac cgcgtcggca
     6301 gaacagggcg tgcaggtaaa agtggccgcg catttgcgtt tatccgcgcg accgacacgg
     6361 gaaaaataaa acgactcgag aagttgcacg gtattcaagt caacccgcat gacataccgc
     6421 ctgtggatgc actcaccggc aagcgctccg gttcggtatt ggaaaatctc aaagacgtgg
     6481 cacaaaaagc caacttatca aaaatactgc cgtatgtgca aacgcttgtc gaggggggat
     6541 tgaccacaac cgacatcgcc gccgctgcaa tcaaaattct cttaggctcg cttgacggcg
     6601 agtatgacac ccacgcgcag tttatttcca ataaagaaga aatgagcaaa caaaacagcg
     6661 ataaaaagca gcacggttcg tttaagaaag ggaaaggccc ccgcaagttc ggtggcaagc
     6721 cgagaagata aaattatcgc tcttgcgcat cacagtatac cgcagctata ttgggatgcg
     6781 ctctatgctt ggcgtttaca aaaaagccaa gccaatcgaa agaccaaccc agtgagcttc
     6841 cattctctgt ccacggaaac cgtgttctgt gtcgataaag cgccacgggg ctcgatagct
     6901 gagcgtcaat tcgagcggaa ttttagtcca tccttcgggg aggatagtta tccccccgcc
     6961 caacccaatc ccaaagaaaa agtttgtttg agaactaaac ccctcaaacc ccatgtacgt
     7021 cagcggacct gcgttgatta aaaaccgcgt cgccagacgg taatggaatg tgaaagcaaa
     7081 ctcatgcttc aattgttgcc gcttgagttc gcccaaagtc attaaaaaac caaagtcgta
     7141 actataaagc caatcccccg cgctgcgtgt aaagggtgtt aaaaattcca agcgcgcacc
     7201 caactgcgtg ccgttgcctg cagcgtctaa aaaactttcg atacgcagcg attgaaagtc
     7261 aatcgggggc ggcgtgggcg gtaatccttc ccgccccaaa tagcccatgc cgtgtaccgc
     7321 tacaaccggt ggcgcagtaa aagcttgtag cggtaaatag gcgcaaataa aactggctgc
     7381 actgcttttt tcagggcatc ccgtcgggga gtcttccttg ctcaaaactg atgcatcgcg
     7441 gtataaaatt cctcccaggt tccaatcgac aagcgaagcc gcattcactg cggtgagcat
     7501 gccgcgttgg tagcctaaaa gacaacgcga cacccgcgcc ggcgatgggc gcagtccttg
     7561 ggggatgtct tcgcaatttt ctaagtgcga ttcaccctta acccgctctg ttttacccaa
     7621 aaggtaagtg tcgccgtaag cgacgaactc aaattggttc tcccgcgtgg caaacgcgac
     7681 aaagacaccg ttcttggaat cgatgtcgtg gtaacgccga gtgacgctca acgaacgacc
     7741 agtatgctcg gtacggatat gcccaccgga gagggcatct tgcatgtaat gcaggccgcc
     7801 accttccaac gcgagtgcag cgacattcga cggcaactcc atcaacacct gatgcgcttc
     7861 gggcgatgta gggttatttt caagcaacga caacaacggt agcaccggtt cgacaagact
     7921 tctctccccg cgtttatgcc attcgacgag ccgacggcgt aatacctcgc gcaacaaacg
     7981 gcaactgtct tcggcgtaag tgggattgtc gtcgctgtcc cagtcgatat tttgaaattg
     8041 cggtaaatcc tcagccaagt cctcggcgac agaatcgtcc aagtcgacgg tgtcttcaca
     8101 ttttgctgcg cttacttgaa gcgcagggta gtgcatcccc gcccaagtgc gatatgcgtt
     8161 tttaccgaaa tgattttggt tgaaaagctg taagtccaaa aaccgtgggt ttttaatggt
     8221 aaagagtgtc gatggatcaa cgggaccggg agaaagtgga gcgcgcttat cctcgctcaa
     8281 aagacttgtg tcgagtttca aactccgttt ttccgcatcc cacacaccaa agaacgctgc
     8341 cacgtcgttt tcgatttcat accaatgagg gtcttcgtcg atgtcgcaac ctttttgggc
     8401 aatcgctcct gcgacgcctg tacctgcatc aggtagaaac gacttccaaa gcaaatcgac
     8461 aaaaccacga tctttcgcgt tgggtaacga ttttattttt ccatactcgg taatgtgatc
     8521 gaccagtgcc gaataatccc ccagcgttgc ggtgtgctta ccgttgaagc gcatctgccc
     8581 gtcgaagttc tttttgccac ttttgcaata cgtgggttgc catttctgag gacacaccag
     8641 cgccaacgct aaataacggc ttttctcacg cacacctcct gcggccacgt tttcagcgag
     8701 aatgcgctgc gctgcgtaac atgcacggtc ggtaaaaaga ctatgctcta aataatcgaa
     8761 tgcgtaagtg ggactcacgc cgacgaggca caaaattata ctgctatgat aaaatacacg
     8821 gcgtgtgaat aaatgtcgaa taaattgcac ctttggaaga accaacacac gcgacactat
     8881 ggaaattaaa ataattcaac tcaacaagag atttgctttt cagcataccg acgtttgcca
     8941 aatgcactca tgaagaaaaa taaccttctt gtcattgctg cgctcaccgg acttgcgatg
     9001 ggtggtcttt ccgcaaagaa acccaagagc aaagcaaagt tcggcgaatg ccacggtatc
     9061 aacgcatgca aaggtcaagg cgaatgcggc agtaaagacc acgcatgcgg cggtatgaac
     9121 gcgtgcaagg gccaaggttg gaagaaaatg acgaaagccg aatgcaaagc aaagaaaggc
     9181 acatttaagc cgctttcgat ggacatgtaa atacacattg cgttacacgc gcatttattt
     9241 tgcgctggca ttactcgccg cgctttgggt gaccttcact ctcttacctc acgaaggcac
     9301 attatatttc gcggcgcgcc acaaacttga tttttccatc gcttgggcat accaatttcg
     9361 cgatgatctt cttttttcgc aggtagacaa atttatgctt tctttactcc cgttttatgg
     9421 aggtaaagtg tggcttgtgg gtcttctttt ggctctgcct cttgttttat ctctcttgtg
     9481 gaacacgccg cgtgcagcgc tctatatagc gcttctttct tctttaatac tctggtttat
     9541 atttttttcc atagcaaatc cgttgtatta tttcaagcgc ctcgaagtga tggggggcag
     9601 cagtctgcgc gatacacttt ttgcgactgc accgaaagac aagataccac tcgatgaaaa
     9661 ttgcgtctat gtaatcaatc ccactgacgt gcgtggcatt agctctgttg aggcactcga
     9721 taaaaaaagt atcatgaagc aaccacgact ttacttgctc gatctaaaag tagtcgaaga
     9781 aaattatgca ctcttaaact tgaaaaacga agtgggaact ccactgttca gcgacgcggc
     9841 gcgcgcatac gcgaccgctg aagttgagta tgcaaaaaaa atacagaaga tgcttttcca
     9901 ggagaagtca atctgccttg tcgcaaactt tgacaaaatg cccgatatag aaaataaacc
     9961 gtactcgcgt gcacggcgca ttttagatgc gcgtaagaag atgaaagtcg atacgaaaat
    10021 aattcaactt ctttaaaatg atttttgaga ttatctaata ggcttacaac cgccctaaga
    10081 cacagccttg atgaaaataa aaaaataacc acctcatctt ttttagtaaa gctgaggtat
    10141 cactgcccat ccgtcacaca acgcagatgc atcgctactt gaactccaat aggcgtcaag
    10201 actcgtcgac gatattcgta ttcgccatag ttcactgaat ctgcgaattt tcgggaaacc
    10261 tccgccagct caggctcaag atcagcgtgt cgttcaagaa tgcattcgcg aatgacggcg
    10321 ccaacaccaa tactttattc gttgcgccgt aaacggcggt cggaataacc gcgataaact
    10381 ataagagcgt gatgagagac aatcgcagta tgcctattgt gctgtgccgt gttgaacaca
    10441 tattgtatat tgcaactgga tttgtgaagc gtcaagagaa aaagagcttc gcgcgcacac
    10501 gttcgcgttc gcgatcgatt tgttcggcag ttgcgccaaa ggtcgacagg ataaattcgg
    10561 tgatcgaaag cgccacctct ccctcgccgg caaaaacggc atccgccccc gccgcgcgca
    10621 ggtcaggtac atcgcgcagg taagcggtgc gtgcgagcac tttgattttt ggattgagct
    10681 gccgcgcttc atggatgaca ttttctccac cacgaatgct cgatgcgctc aaaataagaa
    10741 tatgcgcgtt catcagccct gcacgtttca aggtctcgat gtggcttgcg tcgccataga
    10801 tgacgggcat attgtgcgct ttaagttcgt gcagcgtgtt gaggtttaaa tcgatgacga
    10861 tggggataaa cccattttcc gccaaaagcc gcgagacggt acgccccaca gggccataac
    10921 cgatgacaat tgcatgccgc gtcgcgccgt cgttcagcat ttcactgctg gagtgtggag
    10981 aagtggtatg ccgtgcggca aaatgaaaaa agcggtggag gaaagaaaag cgtcgcaacc
    11041 atttctccag tggcgccgcc gtgcggtaga taagcggatt taaagtgacc gtgatgatcg
    11101 ccaccgcgac caaaatattt gccgcatcgc caggcaagat tttcagctga gcgcccaaga
    11161 ctgccataat aaaagagaac tccgcgattt gcgtaagagc agaactcacc gcgatggcta
    11221 cacgcacggg ataacccaag agagtgagaa taataaacgc acccaacggt tttacaaaaa
    11281 taacgacggc aacggtcgca agcaccaaaa atggagtttt gagaagactc tgcgcgtcaa
    11341 agagcatccc caccgacaca aaaaaaagta ctgcgaacgc gtcgcgcatc ggtatcgcgt
    11401 cggtggctgc acgcaaacta aactcggaac gaccaacgac catgcccgcc aagaaagcac
    11461 ccaacgccat cgacacgccg aaaaaaaatg tggatccgac ggcgatgccc aacgcgagca
    11521 cgagcactgc aagagtaaaa agttcgcgcg aaccggtttg tgcaatcgct gttaaaaatc
    11581 gagggattac ccaaagtccc aagacgaaag ctacgacaaa caacgcgata atttttatca
    11641 atgcaactga gagcgctatt tctaattgct ccaaacttgc tgaagaaaag acaacgggca
    11701 aaagcacaat gacaaagacg gtaaagatat cttccattac aagccatgcg attgataaac
    11761 ggccaatggg tgtgtgtaaa tcgttcgcgt ccaccaagac gcgcagcact acgacggtac
    11821 tcgaaataga aattgccaac ccaaacacaa tgccggattg ccaactccaa gcaaaaaaat
    11881 gtgtcgtaac gcttgctaaa attaccgcag ccaacgcttg taaaaaagca acagggaggg
    11941 caatccgccg tgcagaccag agttctttga aatcgaattg caatcccaca ccgaacataa
    12001 gaagtactac gccaatttct gcaagctgtt cggcgatgtc atgattggca acgaaccccg
    12061 gcgtgttggg gctaatggca atgcctgcga taagatagcc cacaatgggc gagagaccaa
    12121 tgcggtgtgt gattaaccca aaaataagcg cggcggtaaa accggcgacg agggttaaga
    12181 tcaactccac gttgtgcatt ggtgttcctc acaacgcaaa gcagagacac actgag
//
LOCUS       DAOMPD010000016        12120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2272_length_12120_cov_3.252632, whole genome shotgun sequence.
ACCESSION   DAOMPD010000016 DAOMPD010000000
VERSION     DAOMPD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 12120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12120
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_12120_cov_3.252632"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLY93_01185"
     CDS             complement(<1..639)
                     /locus_tag="PLY93_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HRP68130.1"
                     /translation="MANFFLVLLFLGLAALVGKKVGWIHRYRIPPSLTGGLSLLLLFT
                     FLPTYKTTDFFVHLKSLPSEFIALVFACFFLQKHDKAHSKRRSMRDVLAQTSMVWVAV
                     MGQVLIGLVTTVFIFKPFFNEPLAFASVLETGFSGGHGTAVAMGPILAQNGIAAGLEY
                     GLFSATIGLICGIVGGVWLIHHERRRMVKSESAMPESEGVSVDLHRLLVAALL"
     gene            740..1102
                     /locus_tag="PLY93_01190"
     CDS             740..1102
                     /locus_tag="PLY93_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68131.1"
                     /translation="MRRIIIPLSLLTTLSVFAFGAKVVKVIREPKAIILSLEKGFGVQ
                     KDARNELTFTSVKDGKQLKKVTALTGKTAVEDNHYFSRLNAVTLPTTQGKVRVQGRIF
                     YCSFAQKYCSVQKIDEEL"
     gene            complement(1131..1373)
                     /locus_tag="PLY93_01195"
     CDS             complement(1131..1373)
                     /locus_tag="PLY93_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HRP68132.1"
                     /translation="MARMIDLKIPDIGGNDGIELVQWNKKPGDTFRAGDELCELVTDK
                     AAFALEAPQDGKLIAVFQEAKSHPKVGDLAARVEVE"
     gene            complement(1475..2314)
                     /locus_tag="PLY93_01200"
     CDS             complement(1475..2314)
                     /locus_tag="PLY93_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68133.1"
                     /translation="MKKLIAVMAVLAICVSPALFAQNQGNQPNSGAGTATPPSPAEEK
                     EAKWYNPDYRPYVASFDELYKLGDDFANNKFRLALSNYQTGRSILDKMREEVQRNKEE
                     WADAKYLNEKWYWQTIDRKMREERILYEKKSRAKIKAVTYFTRAINHLDEIQNRSFRE
                     KQQMKDLTASVYRDWIISQYDLGNLPQTIDLLERYLKIDPAFETEISPHKYLASGYGF
                     KEAVAEKYNKGTEAERIFYKKNKNKHLLRATELKYKKDSPEYEHILELVNRDEIIATK
                     PSP"
     gene            complement(2524..3831)
                     /locus_tag="PLY93_01205"
     CDS             complement(2524..3831)
                     /locus_tag="PLY93_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP68134.1"
                     /translation="MNTIQEVSSQYNEEHLNKTLADFVIQRREKPDLFAWCEPFYESV
                     EYQYSVGHHMYRRVMLSANANRVVVEDQKTGKAKEMIMMGSNSYLGLNNHPKVKAAAV
                     AAAEKYGAGAGSPPHFSGYYDVHRELEQNLSELKGAEDTILYPSGYSTNVGVLSCFLG
                     PKDTIIIDKLAHASIIDGALLSGAKITTFRHNDMDSLERVLIQAKKGTGDRLVVVEGV
                     YSMDGDIAPLKEIYDLVKKHDAKLMVDEAHSTGVLGKTGKGSPEHFDLEGKIDLVMGT
                     FSKSLGGVGGFISAKKEVVRYLRFFSRAYFFAASPTPMQVAAQNAAVEVLKTEPQWTQ
                     RLWENIKYFHAELKKRGFDIERTQTAITPVVIGDTLVMREIAKFMDEQGVFVNPVPYP
                     AVARKKDRLRLSLSALHTREDLDKVISLLDEAETKFKFVKHEE"
     gene            complement(3874..5487)
                     /locus_tag="PLY93_01210"
     CDS             complement(3874..5487)
                     /locus_tag="PLY93_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP68135.1"
                     /translation="MNGFQKKLQRTWIKFVNGGRENFISAQEAKHLRIANQLLISIFF
                     INLWLNVIEIIAYVFMMRYSAELYRFYLVAYAIPSVILYIATAITYYFINRSGNMNWS
                     FFILPVVVIYIGLLNYFLGPNLMIHLFMFLLLPIPFFLYETRDWKNIVIQECMIVLGI
                     AFIFYAQAILTPPYSVPSFLEKPLAIAAVVTAIAVMLLVAGVMWKQTSVAENQLAIEK
                     EHVETLLGETIPKLERAEEKYRNLVEGTGDIVFSMKSTGEIITINRAVKTHLGFNPQE
                     LVGASIFSLFPDSSQKGLELEADFFRENLRELLENHKPVTSRVIFSGKYAREPVEFSL
                     RMECIETHGDLEILGKAVSVQDDLMIQFLHREKGSYSIGNFINHADVLSQKLTRNLKK
                     YLHPTDVQTLRVSLREILINAIEHGNLAITFDEKSAAQMNGDFLEFLASRRVNPQYEN
                     KKVKVDYYMDARRIAYRIHDDGDGFDHGAMMQKATDIANEDFLAHGRGISMTREFFDI
                     VRYNEKGNEVVLIKYTDKSKGESNLLQIA"
     gene            complement(5619..>6106)
                     /locus_tag="PLY93_01215"
     CDS             complement(5619..>6106)
                     /locus_tag="PLY93_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRP68136.1"
                     /translation="AAVGEEFDVFLGPDQRMQLKRTLKRGDVEKSGLFGSKVEIINQW
                     EIEVANFTKKPRQVLVYDQYPISADPNIATRYNGASRNEDTKDANGILSWKIDLKAGE
                     KTKFDFTYALTFPKETWERFSQGYNNEEAQVQQFYQNSSSKKNVPAAQRSYNLEKMLR
                     K"
     assembly_gap    6107..6119
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6120..7315)
                     /locus_tag="PLY93_01220"
     CDS             complement(<6120..7315)
                     /locus_tag="PLY93_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRP68137.1"
                     /translation="MNRFVVFFILTSVAAFAEVRVKGKIDSVIVFSDRALVKRVQPLE
                     GLEKTGTLRFIDLPQALLGDSVRASATGATVTAVSLRKIEKLYGEEWDEHPLKKKIIR
                     FEADIHHEKDNLETYRDQLKVLDQMMKLTTAQTDKEARVGVLNIDSWDKALVFLDAKK
                     QRYREKIRKSEDVLQTLHKELSKTTIEFNAVTQAAKTSGIEVEVSYAKTDETAKIELE
                     YLVNNVSWIARYDLRGSAEGNEFQLVSHAIIRQSTGEAWKQAKITLSTARPSTSMTPG
                     ILTPWRVDAHEFSSPTAKKQSSGRRLEGESTTIADEAMAETASESTDASETTSVSITL
                     PTRENILSDNADHRVTLGTSALKGTLTHVAVPSLSQYVYLKARLKNMSSAPMVGTMNA
                     FLDGSY"
     gene            7554..7625
                     /locus_tag="PLY93_01225"
     tRNA            7554..7625
                     /locus_tag="PLY93_01225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7586..7588,aa:Pro,seq:tgg)
     gene            7894..7968
                     /locus_tag="PLY93_01230"
     tRNA            7894..7968
                     /locus_tag="PLY93_01230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7929..7931,aa:Arg,seq:tct)
     gene            8118..8190
                     /locus_tag="PLY93_01235"
     tRNA            8118..8190
                     /locus_tag="PLY93_01235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8151..8153,aa:His,seq:gtg)
     gene            8219..8300
                     /locus_tag="PLY93_01240"
     tRNA            8219..8300
                     /locus_tag="PLY93_01240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8253..8255,aa:Leu,seq:tag)
     gene            8409..8480
                     /locus_tag="PLY93_01245"
     tRNA            8409..8480
                     /locus_tag="PLY93_01245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8441..8443,aa:Gly,seq:gcc)
     gene            8529..9977
                     /locus_tag="PLY93_01250"
     CDS             8529..9977
                     /locus_tag="PLY93_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRP68138.1"
                     /translation="MGCFESFSTMPTQQLLRLAIAFFLLTPFTCDTPSKETNLLTLPL
                     KYRAEILDPLYAVDESTAFVVNFLQRRLYTYTPEGALIPDLVSSETRQGHRIVIQMKE
                     NTASADDVVYALTRVARDANQAWVMEQIRSVKKIAPRQVEILLHQSDRPAKTDWMLAK
                     IKLTLPQCAIFRANEHKARGTFVPFSNYRIQEQTGDRILLRGINNYPSIQFLAMPDET
                     ARYFAFREARLDVYEALGIHRKLPYDSTRYEQQTMYDLVVLYAAMHVPKKSALAQSSL
                     RRAINLAFNRKALCEKTLLEACVPADYPVPSVLSSPPKENFPASEEFVLPKEIETVTL
                     FTLGDKERILIAQYMRSIFERFRIRLEFRIVDIPSMVRESNKGTHGIYLMKWAADYPH
                     AENFIVPLFYSKNAGIGGNRAHIKDALLDNLIERASYTPENTARIQQRIRYLSPWIFI
                     GFQNMQFYRLKSSPIRIPKIFAGWSERVIKPK"
     gene            complement(9995..11227)
                     /locus_tag="PLY93_01255"
     CDS             complement(9995..11227)
                     /locus_tag="PLY93_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68139.1"
                     /translation="MIQFVFRNRLWILFTAAFVVLLLAVFRSESNEPGAMQKYYELPI
                     TQLEKIEYAGTMNLTEKEQAQVHYTVIREENILQPKEPLYRIEIKNISSENKNLKTYV
                     ANLQTVKNFYASPLIKTIAQDWSTLDYLYELEHDAKRDEEYGFKNCTNKLTLIFRTQT
                     RIFCIGLASQSDTRRYIFDQSKNKTLIIPDFIARRIQNNIFAQRENSLYPHGVHGIDT
                     IEWSIGKDLIAKFPTLKEKTTGRIALRKMVKEEGKKKIDVWYVENHLGIKPSHAAEAM
                     QFITALRIQKLFATNVQPHDKPLTDFFAALGFTPSAIPAMTGVIKLKKADKQDIILTP
                     FSFFSPNVRPQRTPAFQLENERALSQESIAVSQYNAGYLSADMYPRLTAILTKFEGDL
                     LAEKEKKIGIEKKNKKPK"
     gene            complement(11224..>12120)
                     /locus_tag="PLY93_01260"
     CDS             complement(11224..>12120)
                     /locus_tag="PLY93_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP68140.1"
                     /translation="VRSLFADTLQMSAKEVEKKIPRELGVALGSSSFTPIQLTQMYAA
                     MLNRGERVEPRDLLRIEDNQGRQIWEAPEPPSPQRVMDPAAAYVTLTIMQAVVDNGGT
                     AAYALKSLRNADGMWYFDVAGKTGTTSKYVDAWFVGLVSDEATVMWVGNDNNTTLGAG
                     RSGSGLCAPRFADYIRATRMNDRPKPFAEVFDQTNIVRRSFCSSTGLLSRFPGSCEDE
                     VKDQAFIRGTEPTEFDPSGMPPEEPSPPIDPNAPTNESNPPPEGGESFQNPLDDTNVS
                     PTKKRESNTQKPKILEESGNHL"
BASE COUNT         3167 a         2830 c         2738 g         3372 t
ORIGIN      
        1 aaggagcgcg gccaccaaga ggcggtgcaa atcgacgctt accccttcgc tctctggcat
       61 tgcgctttca ctttttacca tacgtcggcg ctcgtggtgt atcaaccaca caccacccac
      121 gattccacaa atgaggccaa ttgtcgcgct gaaaagacca tattcaagcc ccgcagcaat
      181 accattttga gcaagtatcg gccccatcgc cactgctgtg ccgtgcccgc ctgaaaaccc
      241 cgtttccaac accgaggcga acgccagtgg ctcgttgaaa aatggcttga aaataaaaac
      301 ggttgtaaca aggccaatca atacttgacc catcactgcc acccacacca tcgaggtttg
      361 cgccaacacg tcgcgcatcg aacgccgctt tgagtgtgcc ttgtcgtgtt tttgtagaaa
      421 aaagcaagca aacaccagtg cgataaactc cgaaggcagc gattttaagt ggacaaaaaa
      481 atcggtagtt ttatacgtcg ggagaaaggt aaacagcaac aacaaggaca atccccccgt
      541 gagcgagggc ggaattcgat agcggtggat ccagcctact ttcttaccaa ctagtgccgc
      601 taaacctaaa aacaaaagca caaggaaaaa atttgccatt ttatacggtt aaatttttac
      661 gaatttgtgc gacaaccgta aagtttgtga gcctgctaaa tttttggcag attatagttt
      721 atttaagtat gcttaagtca tgcggcgcat tataattccc ctttctttat taactaccct
      781 ctccgtcttt gcttttggag caaaggtcgt gaaagttatc cgtgagccta aagcgattat
      841 cctttcgctg gaaaaagggt ttggtgtaca aaaagacgcg cgcaacgaac ttacattcac
      901 ctctgttaaa gacggcaaac aacttaaaaa agtaacggca ttgaccggca aaacagcggt
      961 tgaagacaac cactacttca gccggctcaa tgccgttacc ctacccacaa cgcaagggaa
     1021 agtacgcgtc caaggccgga ttttttattg tagctttgct caaaaatact gttctgttca
     1081 aaaaattgac gaagagttat agataatcac ccaacgcgta cgctgtatcg ttactcgact
     1141 tcgacgcgtg cagccaaatc gcccactttg gggtgagact tggcctcttg aaaaactgcg
     1201 ataagtttac cgtcttgcgg cgcttcgagt gcaaacgccg ctttgtctgt caccagctcg
     1261 cagagctcgt caccagcacg aaatgtatcg cccggttttt tattccactg tactaattca
     1321 ataccgtcgt tgccgccaat atcgggaatt ttaagatcaa tcatccgcgc catacttaac
     1381 aaagcagggt ggattgacgg cgtaaactaa aatatataaa taaaaaaacc cgcgctcgcg
     1441 cacgggtttt taagagacga tgagttaggt aaaattatgg cgatggcttg gtcgcaataa
     1501 tttcgtcgcg gttaacaagt tcgagaatgt gttcgtactc tggcgaatct ttcttgtact
     1561 tgagttcggt tgcacgtaac aagtgcttgt tcttgttctt cttgtagaag atacgctccg
     1621 cttcggtacc tttgttatac ttctctgcca cagcttcttt gaagccatac cctgatgcaa
     1681 gatatttatg cggagagatt tctgtttcaa acgcagggtc gattttcaga taacgctcga
     1741 gcaagtcgat cgtttggggt aaattaccca aatcgtattg cgagataatc caatcgcgat
     1801 aaacagacgc tgttaaatct ttcatttgtt gtttttcgcg gaaagaacga ttttgaattt
     1861 cgtcgaggtg gttaatagcg cgtgtgaagt atgtaacagc tttaatttta gcgcgacttt
     1921 ttttctcgta aagaatgcgt tcttcgcgca ttttgcgatc gattgtttgc cagtaccatt
     1981 tttcatttaa gtattttgca tctgcccatt cttctttatt gcgttgaact tcttcgcgca
     2041 ttttatctaa aatggagcgt cccgtttggt agttagaaag cgctaaacga aatttattat
     2101 tcgcaaaatc gtcgccgagc ttataaagtt cgtcaaacga agcgacatac gggcggtaat
     2161 cggggttata ccactttgct tctttttctt cggcgggtga cggaggtgtt gccgtacctg
     2221 caccgctatt gggttgatta ccctggtttt gcgcaaaaag cgctgggcta acgcagatag
     2281 ctagtacagc cattactgca atgagttttt tcatatagtt ctccttcaaa acttttcgtc
     2341 ccttctacct aaaattgctc aaaagataaa ttttgcttag aacaatcgta tagaatgcta
     2401 tattctgtgt cggcttttcc ggtgtaaatc ttaaattagg tttcataaaa aagtaacaac
     2461 agatttaagc gtgagcgctg gaaacacctc gcacccacct aacgacaata attaccaaaa
     2521 attttactct tcgtgcttca caaacttaaa tttcgtttcg gcttcgtcta agagagaaat
     2581 aactttatcc aagtcctcgc gtgtatggag cgcagagagc gataagcgta aacggtcttt
     2641 cttgcgtgca accgcagggt acggcacagg gtttacgaaa acaccttgtt catccatgaa
     2701 tttggcaatt tcgcgcatca cgagggtgtc tccgatgacc acaggggtaa tcgcagtttg
     2761 tgtgcgctcg atatcaaatc cgcgtttttt tagttctgca tggaagtatt taatgttttc
     2821 ccacaaacgt tgtgtccact gtggttcggt cttcagaact tctacagcag cgttttgcgc
     2881 agccacttgc atcggtgtcg gcgacgccgc gaaaaaataa gcgcgcgaga aaaagcgcaa
     2941 ataacgcacg acttcttttt tagcagagat aaatccaccc acgccgccta atgactttga
     3001 gaatgtcccc atcacgaggt cgattttgcc ttcgaggtca aaatgctcgg gtgaaccttt
     3061 gccggtttta cccaacacac ccgtcgagtg tgcttcgtcc accataagtt ttgcatcgtg
     3121 ttttttcacg agatcataaa tttcttttaa gggagcaatg tccccatcca tcgaatacac
     3181 gccttcgacg acgacgaggc gatcacccgt gcctttttta gcttggatga gcacgcgctc
     3241 gagcgaatcc atgtcgttgt ggcggaaagt tgtaattttg gcgcccgaca aaagcgcacc
     3301 gtcgataatc gacgcatgcg cgagcttatc gatgatgatg gtatctttag gccctaaaaa
     3361 gcaagagaga acacccacgt ttgtggaata ccctgaaggg tagagaatgg tgtcttcggc
     3421 gcctttgagt tcggaaagat tttgttcgag ctcgcggtgt acgtcgtagt aacctgaaaa
     3481 gtggggggga gagcctgcgc ccgcaccgta tttttccgca gcagcaaccg ccgccgcttt
     3541 cactttaggg tggttgttga ggccgagata tgagttcgag cccatcataa tcatttcttt
     3601 tgctttgccg gttttttggt cttcgacaac cacgcggttt gcgttggctg agagcatcac
     3661 acgacggtac atgtgatggc cgaccgagta ttggtattct accgactcat aaaaaggttc
     3721 gcaccatgcg aaaagatcgg gtttttcgcg gcgttggatg acaaagtcgg cgagcgtttt
     3781 atttagatgt tcttcgttat attgagaaga gacctcttgg attgtattca tacgttcgca
     3841 tcttttacgc accgcacaac gcgtcaagga agattacgca atctgtagca agttcgattc
     3901 tcccttagac ttgtcagtgt acttaattaa aaccacctcg tttccttttt cgttgtaacg
     3961 cactatatcg aaaaactcgc gcgtcattga aatgccacgg ccatgcgcca agaaatcttc
     4021 attagcaatg tcggttgctt tttgcatcat agcgccatgg tcaaacccgt caccgtcgtc
     4081 atggatacgg tacgcgatac gacgtgcatc catataatag tctactttga cttttttatt
     4141 ttcgtactgg ggattgactc tcctacttgc taaaaactcc aaaaaatcac cattcatttg
     4201 tgctgcgctt ttctcgtcaa aggtaatcgc aaggttgcca tgttcaatcg cgttgatgag
     4261 aatttcccgc aagctcacgc gcaatgtctg tacgtctgtc gggtgaagat atttttttaa
     4321 gttacgcgtc aatttttgac ttaagacatc cgcatggtta atgaaattac caatcgaata
     4381 actccctttt tcacgatgca aaaactgtat cattaaatcg tcttgcaccg aaacggcttt
     4441 accaagaatt tctaaatcac catgggtctc gatgcactcc atgcgcaacg aaaactcaac
     4501 gggttcgcgc gcatatttac ccgaaaaaat aacgcgactg gtgacaggtt tatgattctc
     4561 taaaagctcg cgtaaatttt cgcgaaaaaa atcggcttca agttcgagcc ctttttgcga
     4621 cgaatcggga aatagagaaa aaatgcttgc gccaaccagt tcttgcggat tgaaacccag
     4681 gtgtgttttc accgcgcggt tgatggtgat aatttcaccg gtagatttca ttgaaaaaac
     4741 aatatcaccc gtgccttcaa caaggttgcg gtatttttct tcggcgcgtt cgagtttggg
     4801 tattgtttca ccgagcaatg tttccacatg ttctttttca atcgccagtt gattctcagc
     4861 cacgctcgtt tgcttccaca tcacccctgc gacaagcaac attacagcga ttgccgtcac
     4921 taccgcagca atcgccaaag gtttttccag aaagctgggt acagagtaag ggggagtgag
     4981 tatcgcctgc gcgtaaaaaa tgaatgcgat gcctaacacg atcatgcatt cttgaatcac
     5041 aatatttttc cagtcgcgcg tctcataaag gaaaaacgga atcggcagaa gcaggaacat
     5101 gaataaatga atcataaggt ttggacccaa aaaataattc agtaaaccga tgtagataac
     5161 gacaaccggt agaatgaaaa aagaccaatt catattgccg ctgcggttaa taaaataata
     5221 agtgatcgcg gtcgcgatat aaagaatcac gctcggtatt gcgtacgcaa ccaggtagaa
     5281 tcgatatagc tcggcgctat agcgcatcat aaaaacatac gcaataattt caatgacgtt
     5341 tagccacagg ttgataaaaa aaatggagat gagtaactgg ttcgcaatac ggaggtgttt
     5401 tgcttcctgc gcagaaataa aattttcacg cccaccattg acaaatttta tccacgtgcg
     5461 ctgaagtttt ttctggaatc cattcatgtt taccgcctta aaagcacaga cgcagtgtaa
     5521 accaccgcat cgccaaatga aaaacagttg tctatcgatt tcagatgaga tatttcaaaa
     5581 tataaaactc accccccctg aatagagtga ttctcaagtt acttgcgtaa cattttttcc
     5641 aaattgtagg aacgttgtgc cgcgggtacg tttttcttgg acgacgaatt ttgataaaac
     5701 tgttgtactt gcgcttcttc gttattgtac ccttgtgaaa aacgttccca cgtttcttta
     5761 gggaaagtca acgcataggt gaaatcaaat tttgtttttt cgcccgcttt taaatcaatt
     5821 ttccatgaaa gaatgccatt ggcgtctttg gtgtcttcgt tacggctggc accgttatac
     5881 cgtgtcgcga tattgggatc ggcagagatg ggatactggt cgtatactag cacctgccgt
     5941 ggttttttag taaaattcgc aacttcgatt tcccattgat tgatgatttc cactttactg
     6001 ccaaaaaggc cgctcttttc tacatcgcca cgcttcaatg tgcgtttgag ttgcatacgt
     6061 tggtctgggc ctaaaaacac gtcaaattct tcacccacgg cagctgnnnn nnnnnnnnna
     6121 tatagctgcc atcgaggaat gcattcatgg tacccaccat aggtgcacta ctcatatttt
     6181 taaggcgtgc cttaagataa acgtattgtg aaagcgatgg cactgcaacg tgcgttaaag
     6241 ttcctttgag cgccgacgta ccaagggtaa ctctgtggtc ggcgttgtcg ctcaaaatgt
     6301 tttcgcgtgt cggtagcgtg attgaaaccg atgtcgtctc tgaggcgtcg gtactctccg
     6361 atgcagtttc cgccatcgct tcgtcggcga ttgtcgtact ctccccttcc aaacggcgac
     6421 cggagctttg tttttttgcc gtcggcgatg aaaactcgtg tgcgtctaca cgccacggcg
     6481 ttaaaatacc aggagtcatg ctcgtcgaag gtcgcgcggt tgataaagta attttagcct
     6541 gcttccacgc ttcacccgta gattgacgga tgattgcgtg cgacacaagt tgaaattcat
     6601 tcccctcggc agagccgcgc aaatcatagc gggcaatcca cgagacgtta ttgactaagt
     6661 attctaattc gatttttgcc gtttcgtcgg ttttagcgta cgagacttca acttcgatac
     6721 ccgatgtttt tgccgcctgt gtaacggcat taaattcaat cgttgtttta cttaactctt
     6781 tgtgtaacgt ttgtaacacg tcttcacttt ttcgaatttt ttcgcggtat ctttgctttt
     6841 ttgcgtcgag aaaaacaaga gctttgtccc acgagtcgat gttgagtacg cccacacgcg
     6901 cttctttgtc ggtttgtgcg gtggtgagct tcatcatttg atcaagcact ttaagctgat
     6961 cgcggtatgt ctccaaatta tccttttcat gatggatgtc tgcttcaaaa cgaattattt
     7021 ttttcttaag cgggtgttca tcccactctt ccccgtagag tttttcgatt ttacgcaacg
     7081 acactgcagt gaccgtagcg ccggttgccg aggcgcgtac cgaatcgccc aagagtgctt
     7141 gtggtaaatc gataaaccga agcgtacctg ttttttctaa gccctcaagc ggttgcactc
     7201 gcttgacaag cgcacgatca gagaagacaa tcactgaatc tatcttacct ttaacgcgta
     7261 cctcggcgaa tgcggcaaca gatgtgagaa taaaaaatac aacaaaacga ttcataagcc
     7321 ctccaaaaaa tggaaataat gtagagcata tattaaaaac ctttgccaca cggcaagccg
     7381 tgaaaaaact ataaattttt aaagcggctg tttcaaatct gaagtgcacg aaaaaaagaa
     7441 aaaatccctt tcaagagcct atgcgccaac tcatgcagtg tgcacaaagc ctctgaatag
     7501 gtatcgtcaa atttactcga cgtgatgtct caaactcctc tttcgacgcg atacgggatg
     7561 tagcgcagtg gtagcgcact tgctttggga gcaaggggtc gttggttcaa atccaatcat
     7621 cccgaaattg aacgggacaa tgccgcatag ttgcgcggcc acggagagcc aaggcgcaga
     7681 ccgattctct tcgtgcgcgc gcgtagcgcc gaagacggag aactcgacgt ttcagaggaa
     7741 acgtcgtcgt tcacagtagg attagagcaa cagccttcta agctgtgggt caggggtttt
     7801 aagcggaagg aaggcgccgc atagttgcgt ggctacggag agctaagcgc agaccgattc
     7861 tcttcgtgcg cacacgtaga gaaatcattt agagcgcacg tagctcagta ggattagagc
     7921 aacagccttc taagctgtgg gtcaggggtt cgattccctt cgtgcgcgca tataaatttt
     7981 tttgcatctg cggctatacc gcagatgcaa aagaagaata aaacatggcg gtcgtagcac
     8041 cgaagacgga gagtcgagcg ttttaaaaaa aacgccgcga ctcacagctt ccagcttcgc
     8101 tacaaccgcc aaacatggcg gtcgtagctc agctggtaga gcacaggttt gtggcacctg
     8161 gtgtcgcggg ttcaatcccc gtcgatcgcc cacttcattg aatttggttt acgctacagc
     8221 gagcgtggtg aaactggtag acacgcggga tttaggttcc cgtgccgaaa ggtgtggggg
     8281 ttcaagtccc tccgctcgca tacgatatgc ataggtcaga ggctggcacg aggccatcca
     8341 tgccgacgac cactcgcata attgactttt tttgttttta caaaacaaaa aaatagaata
     8401 cgacccacgc gggaatagct cagtggtaga gcatctcctt gccaaggaga gggtcgtggg
     8461 ttcaagtccc atttcccgct caattaaaag gcgccttttc gagacacctc attgcctcac
     8521 aaccggtaat ggggtgtttc gaatcattct caaccatgcc aacccagcaa cttttacggt
     8581 tagcgattgc cttttttcta ctaactccat tcacctgcga tacaccttct aaagaaacga
     8641 atttactgac gctcccgctc aaataccgcg ccgagatttt agatccactt tatgccgtcg
     8701 acgaatccac agcgtttgtt gtcaattttt tacagcggcg tctttatacg tacacacccg
     8761 aaggagcgtt gatccccgat ttagtttctt ctgaaacaag acaagggcat cggattgtaa
     8821 tccaaatgaa agaaaacaca gcgagcgcag acgacgttgt gtacgcactg acgcgagttg
     8881 cgcgcgacgc aaatcaagcg tgggtgatgg aacaaattag aagtgttaaa aaaattgcac
     8941 cgcgccaagt ggaaattcta ctgcaccaat cggatcgccc cgctaaaacc gattggatgc
     9001 ttgcaaaaat aaaattaaca ttgcctcaat gtgctatctt tcgtgcgaac gagcacaagg
     9061 cgcgcggcac gtttgtgccg ttttcaaatt accgcattca agaacaaacg ggagatcgta
     9121 ttcttttaag agggataaac aattatccga gtattcaatt tctggcgatg cccgacgaga
     9181 ccgcgcgtta ctttgcgttc cgcgaagcgc ggttggatgt ttacgaagcg ttaggcatcc
     9241 atcgcaagtt gccgtacgat tcaacgcgtt acgaacaaca aactatgtac gatttagtcg
     9301 tactctacgc agcgatgcat gtaccaaaaa aatcggcttt ggcgcaaagc agcttgcgcc
     9361 gtgcgattaa cctggcgttc aaccgtaaag ccctttgcga gaaaacattg ctagaagcgt
     9421 gcgtacctgc ggattaccct gtgccttccg ttttatcttc gccgcccaag gaaaattttc
     9481 ctgcatctga agagttcgtc ttacccaaag aaattgaaac agtaactctt ttcaccttgg
     9541 gcgataaaga acgtattcta attgcacaat atatgcgcag catttttgaa cgtttccgta
     9601 ttcgccttga attccgtatt gtcgatatac ccagcatggt gcgcgagagc aataaaggca
     9661 cgcacggcat ttacttaatg aaatgggcag ccgactatcc gcatgcagaa aattttatcg
     9721 tgccgctgtt ctattcaaaa aacgcgggga ttggcggcaa ccgcgcgcat atcaaagacg
     9781 cacttttaga caatctcatt gaacgtgcca gctatacccc tgaaaacacg gcgaggattc
     9841 aacaacgtat ccgttatctt tcaccgtgga tttttatcgg ttttcaaaac atgcaattct
     9901 atcgattgaa gtcgtcacca atccgcatac ctaaaatttt tgcagggtgg agcgagcgcg
     9961 tgataaaacc aaagtaacaa aattttgaat tttattactt tggtttttta tttttctttt
    10021 cgatgcctat ctttttttct ttttctgcaa gcaagtcgcc ttcaaatttt gtgagaatcg
    10081 cggtgaggcg tggatacatg tcggcgctca agtaacccgc gttgtattgc gacacggcaa
    10141 tactttcttg tgaaagcgct ctttcgtttt cgagttgaaa cgcgggtgtt ctttgcggtc
    10201 tgacattcgg tgaaaaaaaa gaaaacggcg ttagtataat atcctgctta tcggcttttt
    10261 tgagtttgat cactcccgtc attgccggaa ttgccgaggg ggtaaatccc aatgcagcaa
    10321 aaaaatcggt gagcggtttg tcgtgcggct gtacgttcgt agcaaaaagt ttttgaatac
    10381 gcagcgcggt aatgaattgc atcgcctcgg ctgcatgtga aggcttgatg cctaaatgat
    10441 tttcaacata ccacacatcg attttcttct taccctcttc tttcaccatt tttcgcagtg
    10501 caatgcgccc cgttgttttt tctttgagtg tagggaactt ggcgatgagg tcttttccga
    10561 tactccactc aatcgtgtcg atgccatgta cgccgtgagg ataaagtgag ttttcacgct
    10621 gcgcaaaaat attattttga atgcgcctcg caataaagtc gggaatgatt aaagttttat
    10681 ttttactttg atcaaaaata taacggcgtg tgtcgctctg cgaagcaaga ccaatacaaa
    10741 aaatccgtgt ctgcgtgcgg aatataagcg tcaatttgtt ggtacaattt ttgaaaccat
    10801 attcttcgtc gcgctttgca tcgtgttcaa gttcgtaaag ataatccaaa gtcgaccaat
    10861 cttgagcaat cgtcttaatg agagggcttg cataaaaatt tttcactgtt tgcaaatttg
    10921 caacatacgt tttaagattt ttattttcac ttgaaatatt ttttatctcg atgcgataaa
    10981 gcggttcttt gggttgaaga atgttttctt cgcggatgac agtatagtga acttgcgctt
    11041 gttccttttc ggtaaggttc atcgtccccg cgtattcaat tttttcgagc tgtgtaatag
    11101 gcagctcgta atacttttgc atcgcacctg gttcatttga ttctgagcga aacacggcga
    11161 gtaacaatac aacaaatgct gcggtgaata aaatccaaag gcggttacgg aaaacgaatt
    11221 gtatcataaa tggttgccac tctcttcaag tattttaggt ttttgagtgt tgctttccct
    11281 ttttttagtg ggacttacat tggtatcgtc caaagggttt tgaaaagatt cgccgccttc
    11341 cggtggtgga ttactttcat tcgtgggcgc attggggtct attggcggcg acggctcttc
    11401 cggaggcata ccggatgggt cgaactcggt gggctctgta cctcgaataa acgcttggtc
    11461 ttttacttcg tcttcgcacg acccagggaa gcgggaaagt aaacctgtcg atgagcagaa
    11521 agatctacgc acaatattcg tttgatcaaa aacctctgca aaaggtttgg gcctgtcgtt
    11581 catgcgcgtg gctcggatgt aatcggcgaa gcgcggagcg caaagaccac tccccgaacg
    11641 accagcgccc aacgtggtgt tgttgtcgtt acccacccac atcacggtcg cctcgtcaga
    11701 aaccaaacct acaaaccacg catcgacgta tttcgacgtg gtgccggttt tacccgcaac
    11761 atcaaagtac cacatcccat ccgcattgcg caacgatttg agcgcatacg ccgctgtgcc
    11821 gccattgtcg accaccgctt gcataattgt gagagtcacg tacgcagctg cggggtccat
    11881 cacccgttgc ggtgatggtg gttcgggagc ttcccaaatt tgcctgcctt ggttgtcttc
    11941 aatacgcaac aagtcgcgcg gttcaacacg ctcgcctcgg ttgagcatcg ccgcgtacat
    12001 ctgagttagt tgaatggggg taaaagaaga agaacccaac gccacaccga gctcgcgtgg
    12061 aatttttttc tccacttctt ttgctgacat ttgcagtgta tcggcaaaga gcgaacgcac
//
LOCUS       DAOMPD010000017        11756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2355_length_11756_cov_2.327152, whole genome shotgun sequence.
ACCESSION   DAOMPD010000017 DAOMPD010000000
VERSION     DAOMPD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_11756_cov_2.327152"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLY93_01265"
     CDS             <1..186
                     /locus_tag="PLY93_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68141.1"
                     /translation="EELKLVHAELKNIYKEIERRNGRFGLCAISSDVDHLLEITKLGS
                     VFDLYKNQDDALDSIED"
     gene            271..1119
                     /locus_tag="PLY93_01270"
     CDS             271..1119
                     /locus_tag="PLY93_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68142.1"
                     /translation="MRALISFFALIFVLSLSASENIPRVDALVDWKANKIFLRAEWRL
                     NGGYVQAARADIRSRLRAALLAKLSGVVEAIYARGEDNNPSHDLSAYWATVRLDSFQI
                     AENSASATLDVALRGSHSLLSYLPERKWDEAWTSSTEATPSVYDKRALNSAYDTDDEE
                     ALLYTGLVIDARELPFTPSLKTQIFTDSGRLLYGAQFLSRATYVKRGAVGFFTAETQS
                     EARRRAGERPLTVPALDLARTSQNSLVISDEDAAKLFAHEGSIQNLRRARVIILLAPN
                     KLQEKY"
     gene            complement(1130..2026)
                     /locus_tag="PLY93_01275"
     CDS             complement(1130..2026)
                     /locus_tag="PLY93_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68143.1"
                     /translation="MQLIIAFFFSLAFTFSLLSITPLSTRAAMHSVENDALRTYCAEK
                     GITIPCTFRFDTAVRHRDIWAFSFILTRGAKRDVERTALRTQLETPYRRVLEIDSDST
                     KAIQYFRETGDKLIPLEAQTFYKETINPKSQIVVKYAPDFLLKGGALDYTVPPLWESE
                     GVSPSTTSSLALQGKSPYRATITGLIRDLDDKPITKLTIESCHTQAGACPQDGFSVDA
                     QKEFMLDFQIKAARKLKHAQPVFKFVVQTAQEKKPIAEIAIPFKPQPNYFLFGVVGFL
                     VGGLIAVMTLMIRSNTKRQQGA"
     assembly_gap    2056..2113
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2377..2386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2387..4727)
                     /locus_tag="PLY93_01280"
     CDS             complement(<2387..4727)
                     /locus_tag="PLY93_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HRP68144.1"
                     /translation="MVYTPFKKRLRFLFLGIFILTFPPHCFNSAKSLSSAARAEGDGW
                     AAGDNVRYDFSITNVHATVTGATVQIAWESEYLSNAVVDWGLQNVGDNKITQNEHTKS
                     HTVSLGILPEGRYRYRITSTTPEGLQATVDNIGNMWFEIHIYPQVSAINATTNQATRQ
                     MTVTWTTDIPTKGFLALGSTTALSDLSADEGDGNATVSHSITIEIPAYDTTFYYQLRS
                     VDEQGDETVSNNAGAPYTVNIASPAPPPTPNGTQTLPYTINTSTIATTPVDYTHSADT
                     STSTQNFIDRYYPATQKEDGNEVYYTFTITKSVTFKAMLTVGGAPTATVDNDVHLLTD
                     LSTVTNKPGADFNTFRTATLYNNSPSFRGDVNIPTSGTITLPAGTYYVVVDAFKNSSG
                     TIKSGHFTLNVQMTEINAADITINIPSLPYVYTDSTHSTATGGTRSINYYPGYSQNES
                     GSEYVYTFTVPVGKKYKVEAALSGMTGGTDIDIHLLSSLSPLTVLGRNDTALTQTLDA
                     GTYYLVADTYVNASGVEKSGAYTLTVNFTDVTTPPVTNPVVAGYLTYWSTSTTSIQWD
                     KLTHLIYFCLEPNADGSVKTTNGWETTAAVTTAKSHNVTVLLSVCLFGASNIATMVNI
                     PSNSTRMVNNIVAQVKARGAHGVDLDFEIPGSSARARLTQFVHELRAAFSAEGNSPDG
                     KPYRIHMALMPIDWANAYDIANFIGDLDYAMVMSYEGHSASSTQAGPTNKLYSPVPPW
                     AQNFSYQYFFDHWLGKMGYQNAPKLLGGVGYYMQDFTTQN"
     gene            complement(4775..5293)
                     /locus_tag="PLY93_01285"
     CDS             complement(4775..5293)
                     /locus_tag="PLY93_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68145.1"
                     /translation="MGCGWGEFTRALAGHEKETLFIALEKKLGRILSSARVGSQQGIS
                     NIRYMVLDVQWFFSGVFTDATFDAVFINFPDPWPKKRHHKHRFITSEFAEELVRITSR
                     ASLFTFATDNYAYAREVCNVFEKSPSWQNTVGAYLAVDRIPQRPVSFFESIHRNEGAP
                     IYILRYKKIELR"
     gene            complement(5443..6036)
                     /locus_tag="PLY93_01290"
     CDS             complement(5443..6036)
                     /locus_tag="PLY93_01290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP68146.1"
                     /translation="MEVYTCDWSSAIQKKRLNAQHDRGICFIPADLSTIEHVRCYIES
                     VAHSFSLSEQSIFELSLVADELVANAILASYAKAGAENILLKWSVVNNVFTLAVLDYG
                     GGFKLSEVFSTLPQGDDLSTFLRSLKEYREARKATIPLNGNLVEHLRFGRGLRIISNL
                     VSRLDIEFHDADGKMLNTPTQNTLGSIVTIEYVAEAA"
     gene            complement(6136..7695)
                     /gene="gltX"
                     /locus_tag="PLY93_01295"
     CDS             complement(6136..7695)
                     /gene="gltX"
                     /locus_tag="PLY93_01295"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRP68147.1"
                     /translation="MKEVRTRFAPSPTGYLHVGGARTALFNYLYARAQKGKFILRIED
                     TDQARSTEQSYHQVLDSLKWLGLEWDEGPEVGGVHGPYRQSERLHLYKEYAEKLVHEK
                     KAYPCFCTTDELDRKKKQREAMGLPYVYDGKCRNLTDAEIHEKKAAGVEHTIRFRTPA
                     KEVVVDDMVQGSVRFDTALIGDFIIVKSDGFASYNYAVVVDDHLMDITHVIRGVGHLS
                     NTPRQIMIYNALGWQDPQWAHVSEIVGSDHKKLSKRHGATPITAFRDLGYTARAFNNY
                     MALLGWSSPDGREYMTIDEQKSLFDVARCSKSPAMFDVFDLTKAAEVDLAQVPIQELE
                     GYLFQKSKLNWLSNQHIRATEDNTYIHEVFPFVQKANVVPQAELNESNERLRGILLSL
                     RVYLDTLGQIKNYLPDFYLPFTPSATAYSASEYFLDEKFVLLSSTFLRLIEEMDGGEN
                     NDTLEENRIKEAIQQVGKELTIKGKPLFMGLRLASTGRLEGLELPVYLSLLGKARVVE
                     RLKKMISVKTA"
     gene            7793..9226
                     /gene="gatB"
                     /locus_tag="PLY93_01300"
     CDS             7793..9226
                     /gene="gatB"
                     /locus_tag="PLY93_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRP68148.1"
                     /translation="MKFIPTIGLEVHCQLNTQSKLFSPAATSFGADANTEVQTVCLGL
                     PGALPVLNREAVVKAVRAGLALGGFIHERSKFDRKNYFYPDLPKGYQISQFFEPYCTK
                     AKIDIDIESGKKTIGITRIHIEEDAGKLMHSEDPAVHESYVDLNRAGTPLIEIVSEPE
                     IDNSDEAVLYLTELKKILEYIEVSDCNMEQGSLRVDANVSVRPEGTSQLGTRVEIKNL
                     NSFKSVKAAIEYEIHRQSELIESGGAVVQETRLYNAARDMTASMRSKEEAHDYRYFPD
                     PDLVPLILKKEQIESIRKELPELAHEKRARYVSEFGLPEYDAGVLTAERATALYFEEA
                     LKAGAPAKKASNWVMVEMMAVIKEKHTALSALFPPTNLAELIHLIDSGMISSKIAKDV
                     FAAMVETGKKPKAIVEEKGMAQMSDEKQIREIILAVMSEHPASVEDFKAGKDRALKHL
                     QGEIMKKTRGKVNPQLANKILSEELAK"
     gene            complement(9241..10026)
                     /locus_tag="PLY93_01305"
     CDS             complement(9241..10026)
                     /locus_tag="PLY93_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68149.1"
                     /translation="MANELTKGLEYLTVLGPGKVIVLLSTMPVEKLTYLSRHIEHKKI
                     LGILREASVRQVSAIVKRTDTASLVQMMNALPAPLMVEILTKLDTDIIILFLSHLETT
                     KLSKIIQGIGVGRLVELLSTIGPERSLMLARTLPTDNLVVLVQRVPMRDLAILTTRLK
                     MEFLRSLIAADKADNLAILIKNVGAKNILDLIDYLSEQEVLRLTKSLKAQKMQGLIYL
                     IHSMKIKAPTKTKAKKKIKVVKKAKKKKSRPTKPKASRSPLPL"
     gene            complement(10069..10734)
                     /locus_tag="PLY93_01310"
     CDS             complement(10069..10734)
                     /locus_tag="PLY93_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68150.1"
                     /translation="MRYVRACFFLLLALNINAAQRISPLALFEFDKALTYHNANDYTA
                     AINHYLKAIALDGTLVSAVVNLAIVYEKVYDSQNARYYYNEAVRIAPYSFSTRYNRGQ
                     FFQKQGKLKAARADYLVALQSRTDDASLYINLAALTLTIFEKENDATLLKDAEKKLKH
                     AERLESNSPALYFNKARLLELKNSPSLARRYYAEAMRRYPLDSLEYKTSAMQCERLSQ
                     RLK"
     gene            complement(10748..11431)
                     /locus_tag="PLY93_01315"
     CDS             complement(10748..11431)
                     /locus_tag="PLY93_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68151.1"
                     /translation="MSTGEIRSSNILRIGTSSLGELEEKVKALRTINLNVKKRRFLVS
                     REDIKLTSGEVIVPKGSDIDISKVMLLKRHFKADHIIKTFQPDEGIVLVSDMSTPVGI
                     QFSMDLVTQVMNIGGGAYEAFIDRVDSFKEFAALYNKALFPKLAIIGYIPMQTERIKE
                     ELAFYQLIARTDPYIRMIEILHTVIKPNPIIPRLRNLPINPDDRDSWKRFILEIITEY
                     TKAYSIEQR"
     gene            11516..>11756
                     /locus_tag="PLY93_01320"
     CDS             11516..>11756
                     /locus_tag="PLY93_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP68152.1"
                     /translation="MIRNVVEDFGAVGVFLVRTLRGVVQKPFRGRKITFEIYSIGWQS
                     IAIILFTGFFTGMVLGLQGYHTLKRFGADGFLGSLI"
BASE COUNT         2914 a         2654 c         2969 g         3151 t
ORIGIN      
        1 gaggaactaa aattggttca tgctgagctt aaaaacatct ataaagaaat tgaacgccgc
       61 aacggccgct tcggcctgtg cgccatcagc tccgatgtgg atcacctgct cgaaattacc
      121 aagttgggct cggtattcga tctctataaa aaccaagacg acgcgttgga ttcaatagaa
      181 gactaaaccc tcgattaaaa actgagcacg aggtatggag cgcagtaaaa aagtgcacga
      241 ggtttatctc aatttgcacg aaaataaact gtgcgcgctt taatatcttt ctttgcgtta
      301 atttttgtac tctctctctc agcttctgaa aacataccgc gcgtcgacgc gctcgtcgac
      361 tggaaggcga ataaaatttt tctgcgcgcc gaatggcggt taaatggcgg gtatgtgcaa
      421 gctgcccgcg ccgatattcg atcgcgtcta cgcgcagcgc tcttggcaaa gttgagcgga
      481 gtggtcgaag ccatttatgc gcgcggtgag gataataatc catcgcatga tttatcggcg
      541 tattgggcga cggtccgctt agactcattt cagattgcag aaaactcggc atcggcgaca
      601 ctcgatgtgg cgttgcgtgg tagccattct ttgttgtcgt atttacccga aagaaaatgg
      661 gacgaagcat ggacttcttc gacagaggcg acaccctctg tttacgataa acgcgcgtta
      721 aatagcgctt acgacacaga cgatgaagag gcattattgt acacggggct tgtcatcgat
      781 gcgcgagaat tgccttttac gccttctctt aaaacccaaa tttttaccga ttccggacgt
      841 ctcctttatg gcgcacaatt tttgtcgcgt gcgacgtatg taaagcgtgg cgcggtgggt
      901 ttttttaccg ctgagacgca aagcgaagca cggcggcggg cgggagagcg cccccttact
      961 gtgcctgcgc tcgatttggc gcgcacgagc caaaattcac ttgtcattag cgacgaagac
     1021 gcggctaaac tcttcgcaca cgaaggcagc atacaaaatt tacggcgcgc tcgggtgatt
     1081 attctcttag cgcctaataa attacaagaa aagtattaaa agttttactt tacgcgcctt
     1141 gttgccgctt tgtgttactg cgaatcatca acgtcatcac tgcaataaga ccaccgacta
     1201 aaaaaccaac gacgccaaaa agaaaataat tcggctgcgg cttgaaagga atcgcaattt
     1261 cagcgattgg ttttttctct tgcgccgttt gaacgacaaa cttaaaaact ggttgcgcgt
     1321 gttttaactt acgcgccgct ttaatttgaa aatcaagcat aaattctttt tgcgcatcta
     1381 ctgaaaatcc gtcttgaggg caagcaccgg cttgagtgtg acaggattca atcgtgagct
     1441 ttgtaatggg tttgtcgtct aaatctcgaa ttagccctgt gatcgtcgct ctatagggag
     1501 atttaccttg aagcgcaaga gacgatgttg ttgaaggcga taccccttcg ctttcccaaa
     1561 ggggaggcac agtataatcc aaagcgccgc cttttaaaag aaagtcagga gcgtatttca
     1621 cgacgatttg gcttttgggg ttaatcgttt ctttatagaa ggtttgtgct tcgagcggta
     1681 taagtttgtc acccgtttca cgaaagtatt ggatcgcttt tgtcgagtcg gaatcgattt
     1741 ccagaacgcg tcgatacggc gtttcgagct gcgtacgcaa agctgtcctt tcaacatcac
     1801 gttttgcgcc tcgggtcagt ataaacgaaa acgcccaaat gtcccggtgg cgcactgccg
     1861 tgtcaaagcg gaatgtgcag ggaatagtta tccctttttc ggcgcagtag gttctgagag
     1921 catcgttttc aacgctgtgc atcgccgcac gggtacttaa aggggttatg gacaaaagcg
     1981 aaaacgtgaa agcgagcgaa aaaaagaaag caataataag ttgcatcgag gcatactttc
     2041 aaagaaactt gtgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnntgtcata gttgagtgcc cagataccga taccacccat ttgccgcgat
     2161 ttgatgtagt tgtacttggt gttgaggctt gcgcgggtgt cgtaccacac ttgatggtat
     2221 gcaccgtctt tcttgtagaa ataatatgga ttcttgatgg tgttttcata acctaagatg
     2281 acattcacgc catcgggtac agcggcgttt attatgggga tgatggatga atacgttttg
     2341 gttttaaccg tcgaagctcc taaatttttt gcagggnnnn nnnnnnaatt ttgggtagta
     2401 aaatcttgca tatagtagcc cacgccaccc aaaagtttag gcgcgttttg gtagcccatt
     2461 ttccccagcc agtggtcgaa gaaatactgg tatgagaaat tttgcgccca tggaggtacc
     2521 ggcgaataga gtttattggt ggggcctgct tgtgttgagg atgcgctatg cccttcgtag
     2581 ctcatcacca tcgcatagtc caagtcgcca atgaagttgg caatgtcgta tgcattcgcc
     2641 caatcgatgg gcatgagcgc catgtgaatg cggtatggtt ttccgtcggg ggaattccct
     2701 tcggcgctga acgcagcgcg tagctcgtgt acaaactggg tgagcctcgc cctggccgat
     2761 gagcccggta tttcaaagtc gagatcaacg ccgtgtgcac cgcgcgcttt tacttgtgcg
     2821 acgatgttgt tcaccatacg cgtgctgtta gagggaatat tgaccatggt ggcgatattg
     2881 ctggcgccaa aaagacacac cgacaaaagc acagtcacat tgtgtgattt agcggtcgtg
     2941 actgctgcgg tggtctccca tccgtttgtc gtttttacgc taccgtcggc gttaggttct
     3001 aaacagaaat aaataagatg cgtaagttta tcccactgga tcgatgtcgt cgatgttgac
     3061 cagtacgtca aatagcccgc aacgacggga tttgtcacag ggggggttgt cacatcggtg
     3121 aaattaacgg tgagcgtgta tgcgccactt ttttccaccc ccgacgcgtt cacgtacgta
     3181 tcggcgacaa gatagtatgt gcctgcgtcg agagtttgcg ttaatgcagt gtcattgcgc
     3241 cccaacacgg tgagcggtga aagcgacgat agcaaatgga tatcgatgtc ggtaccgcca
     3301 gtcatccctg aaagcgccgc ttcgacttta tattttttcc caaccggaac ggtgaatgtg
     3361 tacacatact cagaccccga ttcattttgc gaatagcctg ggtaatagtt gatactgcgt
     3421 gtgccaccgg ttgccgtgct gtgcgtgctg tcggtgtaaa cgtatggcaa cgacggtatg
     3481 ttgatggtaa tgtcggcggc attgatttca gtcatttgta cgttgagcgt aaaatgcccg
     3541 cttttaatcg tacctgagct atttttgaaa gcatcgacaa cgacgtagta cgtgccggca
     3601 ggtagcgtta ttgtaccgct tgtgggtata ttaacatccc cgcgaaacga aggggaattg
     3661 ttatagagtg tggcggttct aaatgtgttg aaatctgccc ctggtttgtt cgtaaccgtt
     3721 gagaggtcgg ttaagaggtg tacgtcgttg tcaacggttg ctgtgggcgc accgcctacg
     3781 gtgagcattg ccttgaatgt gaccgatttg gtaatcgtaa aggtgtaata cacctcgttg
     3841 ccgtcttctt tttgtgtcgc aggataatag cggtcgatga aattttgcgt gctcgtggag
     3901 gtatctgcgc tatgcgtgta atccaccggc gttgtggcta tcgtgctggt gttgatggta
     3961 tacggtaacg tttgggtgcc gttgggggtg ggcggcggtg cggggcttgc gatattcact
     4021 gtatagggcg cccctgcatt gttactcacc gtttcatcgc cctgttcgtc caccgaacgc
     4081 agttgataat aaaacgtcgt atcgtacgca gggatttcaa tggtgatcga gtgcgacacg
     4141 gtcgcattcc cgtcgccttc gtcggcgctt aaatcgctga gggctgtcgt gctccccaga
     4201 gccaaaaaac cctttgtggg gatgtccgtc gtccatgtta ctgtcatttg gcgcgtggct
     4261 tggttcgttg tggcgttgat cgccgacact tgagggtaga tgtgtatctc aaaccacatg
     4321 ttgccaatgt tatccacggt ggcttgaagt ccctcgggag tggtggaagt gatacggtag
     4381 cggtagcggc cttcgggaag aatccccagt gaaaccgtat gcgattttgt gtgctcgttt
     4441 tgagtaattt tgttgtctcc gacgttctgg agtccccaat cgacaacggc attcgataag
     4501 tactcgctct cccacgcaat ttgaacggtg gcgccggtca ctgtggcatg cacattggtg
     4561 attgaaaaat cgtagcggac attatcgccc gctgcccaac cgtcgccctc agcgcgtgca
     4621 gcgctggaaa gcgatttggc agaattaaag cagtgcgggg ggaacgtaag aataaatatc
     4681 cctaaaaaca aaaagcgtag tcgtttttta aaaggagtat aaaccacgct atatatattg
     4741 ccatgcgaac gtactcttgt gccaaatttg agatctaacg taattctatc tttttatacc
     4801 gcaaaatata aatcggtgcg ccttcgttgc ggtgaatact ttcaaaaaaa ctcaccggcc
     4861 tttgggggat gcgatcaacg gctaaatagg cgcccacggt attctgccaa ctcgggcttt
     4921 tttcaaaaac attacacact tcgcgtgcgt acgcatagtt atcggtggca aaagtaaaaa
     4981 ggctggcgcg cgatgtgatg cgcacaagct cctctgcaaa ctcagaggta atgaaacggt
     5041 gcttgtggtg gcgcttttta ggccatgggt cggggaaatt gataaacacg gcgtcaaaag
     5101 tagcgtcggt aaaaacccca ctaaaaaacc attgcacgtc gagcaccatg tagcggatat
     5161 tggagatacc ctgttgactc ccgacacgcg cactcgaaag aatgcgtcct aatttttttt
     5221 caagcgcgat gaatagtgtt tctttttcgt gcccagctaa cgcacgcgta aactcgcccc
     5281 agccgcagcc aatttcaaga gtgaaatttt tgtaaccggg gaaagcagcg cggatgtcga
     5341 gcgggaaatt agcttcatta aagggtaggg taaactcacc accacgcttt gctttctgcg
     5401 ttaaaattgt ggcaagcttt tgatgatacg tatttttggg attcaggctg cttccgccac
     5461 gtattcgatg gtgacaatag aacccaacgt gttttgtgtg ggtgtgttta gcattttccc
     5521 gtcggcgtca tggaactcaa tgtcgagtcg actgactaag ttggagatga tgcgcaagcc
     5581 acggccaaaa cgaagatgct cgacaagatt tccattgagt ggaatcgtgg ctttacgcgc
     5641 ctcgcggtat tctttaagcg agcgcaaaaa agtcgataaa tcgtcgcctt gggggagtgt
     5701 gctaaaaact tcggaaagtt tgaagccgcc gccgtaatcg agcacagcca gtgtaaaaac
     5761 gttatttacc accgaccatt tgagtagaat attctccgcg cctgctttgg cgtagcttgc
     5821 gagaatagcg ttcgcgacca attcgtccgc gacaaggctc agttcaaaaa tactttgttc
     5881 tgaaagagaa aacgagtgcg cgaccgactc tatatagcag cggacgtgtt cgatggtgga
     5941 taaatcggcc gggataaaac aaatgccgcg gtcgtgctgc gcgttgaggc gttttttttg
     6001 tattgctgat gaccaatcgc aagtataaac ttccatatta cttcttggag aacttgtgca
     6061 aaaatttgca ataatcatgg gcattgcatt tcttgtcgta tccacagttt cccccttttt
     6121 tcaatgttaa gtccatcaag cggttttcac gcttatcatc ttcttgagac gctctacaac
     6181 gcgtgcttta cccagtaaag ataaatacac gggcaactca agcccctcta aacggccggt
     6241 ggaagctaga cgcaacccca taaaaagagg ttttcctttt attgtgagtt ctttgcccac
     6301 ttgttggatt gcttctttta tgcggttttc ttcgagcgta tcgttgtttt ctcccccgtc
     6361 catttcttct atgagacgca agaaagtgga agataacaac acaaattttt cgtctaaaaa
     6421 gtattcagag gcgctatacg ctgtagcaga cggcgtgaag ggtaagtaga aatcgggtag
     6481 ataattttta atttgcccca acgtgtctaa atagacgcgc aaggaaagaa gaattccccg
     6541 caagcgctcg ttgctttcgt tcaactccgc ttgtggcacc acgttcgctt tttgcacaaa
     6601 agggaagact tcatggatat acgtattatc ctcggtcgcg cggatgtgtt ggttcgagag
     6661 ccagttgagt ttcgatttct gaaaaagata cccttcgagt tcttggatgg gtacctgcgc
     6721 taagtcgacc tcggcggctt tggttaagtc gaacacatcg aacatcgccg gtgactttga
     6781 acaacgcgcc acgtcgaaga gcgacttttg ttcgtcgata gtcatgtact cgcggccatc
     6841 gggcgacgac caacctaaaa gcgccatata attgttgaat gcacgcgctg tgtaccccaa
     6901 gtcgcggaac gcagtgatgg gggttgcgcc gtgccgcttt gaaagttttt tgtggtcgga
     6961 acccacaatt tcactcacgt gcgcccattg gggatcttgc caacccaacg cgttgtaaat
     7021 cataatttgg cgcggcgtgt tcgagagatg ccccactcca cggatgacgt gcgtgatgtc
     7081 catgaggtga tcgtccacca cgacggcata attataggaa gcaaagccgt cggatttcac
     7141 gatgataaaa tcgccgatga gcgcggtgtc aaaacgcacg ctcccttgca ccatgtcgtc
     7201 gacaaccacc tcttttgcgg gggtgcggaa acgaatcgtg tgctcaacac ccgctgcctt
     7261 tttttcgtga atctcggcgt cggttaaatt gcggcacttg ccgtcgtaca cgtagggtaa
     7321 ccccatggct tcgcgctgct tttttttgcg atcgagttcg tcggtagtgc aaaaacaggg
     7381 gtatgctttt ttttcgtgaa ccagtttttc cgcatattct ttgtaaagat gaagcctttc
     7441 cgattggcgg tacgggccgt gtacaccacc cacttcgggg ccttcgtccc attcgagacc
     7501 aagccatttc aaagaatcca aaacttggtg atagctttgt tcggtcgagc gcgcttggtc
     7561 ggtgtcttcg atacgcaaaa taaatttccc tttttgcgcg cgtgcgtaga gataattgaa
     7621 aagcgcggtg cgtgcaccgc cgacgtggag gtatccagtg ggcgaggggg caaagcgtgt
     7681 acggacttct ttcactcggt ttgagtaaga gatttagaca ggtggtcaat gaggttatag
     7741 attccttacg acttcttcgt cgacgcgctg tttcacgctt aaaaaatgag ccatgaagtt
     7801 tatacccacc atcggcctcg aagtccactg ccaactcaat acacaaagta aacttttttc
     7861 tcccgcagcc acgtcgtttg gcgccgatgc caacaccgag gtgcaaaccg tgtgtttggg
     7921 actccccggc gcattacccg tgctcaaccg cgaagcggtg gtgaaagcag tgcgtgcagg
     7981 ccttgcgttg gggggattta tccacgaacg cagtaagttc gaccgtaaaa attattttta
     8041 tcccgactta cccaaaggat accagatttc acaatttttt gaaccgtatt gtaccaaagc
     8101 aaaaattgat atcgacattg agtcgggtaa aaagacgatt ggaattacgc gtatccatat
     8161 cgaagaagac gcgggtaagc ttatgcactc ggaagatccg gcagtccacg aaagttatgt
     8221 ggacctaaac cgcgccggca caccgctcat cgaaattgta tcagagcctg aaatcgataa
     8281 ctccgacgaa gctgtgcttt atctcacgga attaaaaaaa attctggagt atatcgaagt
     8341 ctccgattgc aacatggagc aggggagttt gcgcgtcgat gcgaacgtga gcgtgcgtcc
     8401 cgaaggaaca tcgcaactcg gcacccgcgt tgaaattaaa aacttgaact cgtttaagtc
     8461 cgtcaaagcg gcgattgaat acgaaatcca ccgccaaagc gaactcatcg agtcaggagg
     8521 cgctgtggtg caagagacgc gcctttacaa cgcggcgcgc gacatgaccg catcaatgcg
     8581 ctctaaagaa gaagcgcacg actaccgcta tttccccgac cccgatctcg taccgctcat
     8641 cttaaaaaaa gaacaaattg aaagcatacg caaagagttg ccagagctag cgcacgaaaa
     8701 gcgcgcgcgc tacgtgagcg agtttggtct ccctgaatac gatgccggcg tgttgactgc
     8761 cgaaagagcg acagcgctct actttgaaga agcgcttaaa gcgggtgcgc ctgcgaagaa
     8821 agcgtcgaac tgggtgatgg tggaaatgat ggcggttatc aaagaaaagc acaccgcgct
     8881 atccgcactt ttcccgccga caaatctagc cgaacttatt cacctcatcg actcaggtat
     8941 gatttcaagt aaaattgcga aagacgtttt tgcagccatg gtggagacgg gcaaaaaacc
     9001 taaggccatc gtcgaagaaa aagggatggc gcaaatgagc gatgaaaaac agatccgtga
     9061 aattatactc gcggtgatga gcgagcatcc tgcgtcggtc gaagatttta aggcaggtaa
     9121 agatcgcgcg ctcaaacacc tacagggtga gattatgaaa aaaacacgcg gtaaagtcaa
     9181 tccgcagctt gccaataaaa ttttaagcga agagttagcg aagtaaaact gtcacgttcc
     9241 ctacaaaggc agaggtgatc tgcttgcctt cggcttcgtt ggacgactct ttttcttttt
     9301 tgcctttttg accactttta tttttttctt tgcttttgtc ttagtggggg ccttgatttt
     9361 catactgtgg attaagtaaa taagtccttg cattttttgt gctttcaagc tcttcgttaa
     9421 acgcaacact tcctgttcgc ttaagtagtc aatcagatca agaatgttct tcgcgcctac
     9481 attttttatg agaatagcta aattatccgc tttatcggcg gcaataaggc ttctcaaaaa
     9541 ttccatttta aggcgcgttg ttaaaattgc caagtcgcgc atgggcactc gttgtaccaa
     9601 aactacaagg ttgtccgtcg gtaatgtgcg tgccaacatc agcgagcgct ctggaccgat
     9661 tgtggaaagc aattcgacga ggcgtccaac accgatgcct tggataattt tagagagctt
     9721 ggtggtttct aagtgcgaca aaaaaagtat gattatatcg gtatcaagtt ttgttaaaat
     9781 ttcaaccatc agtggcgcag gtagtgcgtt catcatttgc actaaggatg cggtatcggt
     9841 gcgcttgaca atcgcactga cttgcctcac cgacgcttcg cgtaaaatac ccagaatttt
     9901 tttgtgttcg atgtgacgcg aaaggtaggt gagcttttca acgggcatcg ttgaaagcaa
     9961 tacaatgact ttaccgggcc ctaaaacggt gagatattcc aaacccttag tgagttcgtt
    10021 tgccatgcga cataaggtaa gacgcccaac agcgcgaaaa gttgcatttt attttaagcg
    10081 ttggctcaat cgttcgcatt gcatagcgct tgtcttatat tcgaggctat ctaaaggata
    10141 acgccgcatc gcttctgcat agtaacggcg ggcaagcgaa ggagaatttt ttagttctaa
    10201 aaggcgggct ttattaaaat agagggcggg gctattcgat tcaaggcgtt cggcgtgttt
    10261 gagttttttc tccgcatctt ttaataaagt agcgtcgttt tctttttcaa aaatggtgag
    10321 cgtcaacgcc gccaagttga tgtagagcga agcgtcgtcg gttctgcttt ggagcgcaac
    10381 gaggtaatct gcgcgcgctg cttttagctt tccctgcttt tggaaaaatt ggccacggtt
    10441 gtagcgcgtg ctgaaggagt atggagcgat acgcaccgct tcgttgtagt aatagcgtgc
    10501 gttttggcta tcgtatactt tttcatacac aatcgccaaa ttaacgacgg ctgagactaa
    10561 tgttccatct aaagcaatcg ctttgaggta gtgatttata gcggctgtat aatcgttggc
    10621 gttgtggtaa gtaagcgctt tatcaaattc aaaaagcgca agcggggata tcctctgcgc
    10681 cgcgttgata ttcaacgcga gtagaagaaa aaagcacgcg cgtacataac gcatacttta
    10741 ttatcgacta tcgctgttca atcgaatagg cttttgtgta ctcggtgata atctccaaaa
    10801 taaaacgctt ccacgaatcg cggtcatcgg ggttaatcgg taagttgcgc aaccgtggta
    10861 taatggggtt cggtttaatc acggtgtgta gaatttcaat catgcggata taggggtcgg
    10921 tgcgcgcgat gagttgatag aacgccagtt cttctttgat acgctctgtt tgcatcggta
    10981 tataaccgat gattgccaat ttagggaaga gcgctttatt gtagagcgcg gcaaattctt
    11041 tgaacgaatc gacacggtcg ataaacgctt catacgcacc gccgccgata ttcatcactt
    11101 gagtgactaa atccattgaa aactggatac ccaccggcgt actcatgtcg gaaaccaata
    11161 cgatgccttc gtcgggttga aaagttttga tgatgtgatc ggccttgaaa tggcgtttta
    11221 agagcatcac tttcgagata tcgatatcgc tccctttggg gacaatcact tcaccgcttg
    11281 tgagtttaat atcttcacgg cttactaaaa aacgccgttt ttttacgttg aggttaatcg
    11341 tccgtaacgc cttgactttt tcttccaatt cacctaaaga actggtacca atgcgtagga
    11401 tattggacga tcgaatttcg ccggtcgaca tagaaggtta ttccttactc ggtcatgggt
    11461 gtgacaatta aataatgact tttcactctt tctcaaaaat aggtctatac caagcttgat
    11521 acgtaacgtg gttgaagact ttggcgcagt gggggttttt ctcgtgcgca ctctgcgggg
    11581 cgttgtacaa aaaccgttcc gagggcgcaa aattaccttt gaaatctatt cgattggctg
    11641 gcaatcgatt gcgatcatcc tttttacagg tttttttacc ggcatggtat tgggactcca
    11701 aggttaccac accttgaagc ggtttggcgc cgatggtttt ttaggctcgc tcattg
//
LOCUS       DAOMPD010000018        11403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2453_length_11403_cov_3.016831, whole genome shotgun sequence.
ACCESSION   DAOMPD010000018 DAOMPD010000000
VERSION     DAOMPD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_11403_cov_3.016831"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLY93_01325"
     CDS             <1..345
                     /locus_tag="PLY93_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68153.1"
                     /translation="EATYRLIPNRNMFNTRFLDRVIVKGKSEPIPILEILDGKNDIRT
                     ERKLTHRAKFEEGAYAFHAGKADIALKIFQELSAALIEDPACELYLERCQLRIAGKKI
                     EGVDEEYKKKAL"
     gene            396..1250
                     /locus_tag="PLY93_01330"
     CDS             396..1250
                     /locus_tag="PLY93_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRP68154.1"
                     /translation="MINIIDGKIKDLGDGFQVRRILPHGSLRTVGPFVFVDHMGPVKI
                     ERDKALTVRAHPHIGLSTVTYLYEGVIHHKDSIGSDVLIYPNEVNWMTAGRGIVHSEH
                     SRLDPQFTNLHGMQVWVALPKEYEEVNPTFEHYDAAVLPEIKNDAYTLRIIAGSFMGQ
                     SSPVKTHSPLFYADLQLKKNIKIPLDFPRGHDAALYVARGEIRVSGQSVLTGAMIALT
                     KDTPCEVEATEDARIAILGGEPLKDPRHLWWNFVSTSKERIEEAKEAWRKNAMGAVAG
                     ETDRIPLP"
     gene            1296..1853
                     /locus_tag="PLY93_01335"
     CDS             1296..1853
                     /locus_tag="PLY93_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68155.1"
                     /translation="MEIKRSEMLQWAQSAQLNAESHNDVQTERAHTTSASRHTTVSID
                     ALRTKVLGLQQEVHDLQTGISSRQIQMSFLNQVGEGSAWQKELRRFMNEQFPSLYLEL
                     KENQNLDEFKAETTTSITQLRENLLKKEIQIQNIISAGIFEPTQGNDTDEMAAANNSE
                     IIKDYTASRDMFTKLRPDTVKNLLH"
     gene            1863..2156
                     /locus_tag="PLY93_01340"
     CDS             1863..2156
                     /locus_tag="PLY93_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRP68156.1"
                     /translation="MKNNVTRIDSTLLKDVLRACALEYKGEGAPIVKAYAEGEAAQRM
                     LEIARKHGVQIQEGQEESLLLALKSVKLNTQIPTEIYLAVARIYAYLIGKKKE"
     gene            2206..3399
                     /locus_tag="PLY93_01345"
     CDS             2206..3399
                     /locus_tag="PLY93_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRP68157.1"
                     /translation="MTRYQVKLLRPGVSFTKPVYIDPTNMLAAANVIVTQSDIDRLVK
                     WKIEEVLSEGEPVYANKKDESAFMDGQQKIDIENIKRELKNLLRAKPGFNNLTEASEA
                     ILKNFYERVNQNNNPVTNDVRNKAEEITNFVTDNPLLVIYVLQYIIETSTYKHVIACA
                     IYSVRLGLALDFSRPKIVELVFAILMMDVGMLKVPSTIIEKKEKLNETELQIIHAHTV
                     HGYQMLTQTAKIKNSIASVALQHHEHFDGSGYPRKIKGAEMAEYARVASILDSFCAII
                     SEKPYRKNRLPYEAMKELLTLGIYRYDPIYLKTFLDSQAIYPLGSIVELSDGSTGMVV
                     MASKGKPMRPVVWILRDAKNQRPKQLEFVHLLYTTDKFISRAKYPQDADLNLDLEFTQ
                     LSKLL"
     gene            complement(3401..4108)
                     /locus_tag="PLY93_01350"
     CDS             complement(3401..4108)
                     /locus_tag="PLY93_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRP68158.1"
                     /translation="MIQNPFEKFTNTIPFRFDDDDDDDDKKKKKDKESEGAAPLGERL
                     AERFLENRQIFLWGPVTDRSAQSIVERLLFLESNDPGKPIYFFINSPGGVITSGMAIY
                     DVMKMISSPVYTITMGMAASMGAILLTVGEKKHRYVFQHAKVMIHQPLISGQIVAPAL
                     DIKIQAEEIKKTREELNRIIAETTGQPLSRIEKDTDRDYYLDGKGAVEYGLADKVLTS
                     FSELGLFAKDKPKKSKK"
     gene            4264..5625
                     /locus_tag="PLY93_01355"
     CDS             4264..5625
                     /locus_tag="PLY93_01355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP68159.1"
                     /translation="MENLKNKVVLITGGAGGIGLNTAAFFAEAGCRLVLTDLNEAALV
                     NAERRLAKHNVLILTQAMDATDLKAVETLRDKIEFQFGGVDILVNNAGIGFNAEIADT
                     SITKWRQLMDLHFWAPLYHIQTFLPAMKARKSGHIVNVSSGQAYFRAPTWGAYSVTKL
                     AIGAMSEILSVEVARDNVHVSTVYPFLVNTGFYNTASASSFGEKLFLQFLPLYSHKPE
                     EVAALIFNAVRENKTVEMVSVFNQFFKYMRVVNPLASVIDWMTLTFMSKKQQDAFGFS
                     SLLERWTETMNSILVNIKNHAPKKGFIIHEVMTGEHEFTEGFGGKGKHEFFFEADWGV
                     DNLIDFLNIADNSKFLVSTLRGTVTVGGFCQNVPMQGTIELRYLQEQKIRYTFEFEVS
                     GERYRYIGEKRDIYPWNLLYSHTTCFGTVTKLSTGEVVSNSVTHFLMESLPQFLGSLS
                     ITG"
     gene            5772..6509
                     /locus_tag="PLY93_01360"
     CDS             5772..6509
                     /locus_tag="PLY93_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HRP68160.1"
                     /translation="MSIWQRFKRAMRSIFGGWVSEMEDPSLILEQNIRDMRDKIPKMN
                     ENIAMLKANAKLLEKEKQKLLSEQEKLVANVKAAIGQNREDIAGEYALQLEQNKTKVQ
                     QVEQNLAQAQTAAVKGEEAKVSFMKELDRKTKEALDAIESAKRAKWQKEVADTMEQFT
                     AGRVDHTHDEMVKRIERDGAMADAKLDMALNKEDKGRAKIEADAEKIRAAELVKQFKV
                     EMGVAGTSAPASTTSEGGSQEQAGVRN"
     gene            complement(6760..7515)
                     /locus_tag="PLY93_01365"
     CDS             complement(6760..7515)
                     /locus_tag="PLY93_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRP68161.1"
                     /translation="MQPTLKNTNQPMSENLLTVCGFSSVKAIAQHTPERIQRLFFDEA
                     SAPLFAQTCKYLAETHKIYRMVTTDELKRIAVTAHHQGVAAVITKTKPKKLDILNHHT
                     LCLHNVENPHNVGAILRTAAFFGVERICVSRSTYVAAETAAAWRVAEGGLTHVKLWVY
                     DNDEEFFTWAKEKNYRALAAVKPTAKTQRTLEEFLRDKKTSPLVVCLGNEEEGLPSLF
                     VAQCHGHFSIRGSGRIESLNVSVTAALCLDRFS"
     gene            complement(7648..8679)
                     /locus_tag="PLY93_01370"
     CDS             complement(7648..8679)
                     /locus_tag="PLY93_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRP68162.1"
                     /translation="MTATATLTKKDFTSNQTVRWCPGCGDYGILTAVQKTMPEIGVSK
                     ENIVFISGIGCSSRFPYYMETYGFHSIHGRAPAIASGLKISRPELSVWVITGDGDSLS
                     IGGNHLIHVLRRNIDINILLFNNEIYGLTKGQYSPTSARGTIAKTTPEGSIDTPFSAL
                     RLAQGAGGTFLARTYDKDMKHMEKIFKESHNHAGTSLVEILQNCVIFNDEVFEPIVGK
                     ENRANTMIFVEQGEKLLFGAEKEKGLHFNEKGTFDVVSAVDNPNAVSTFDAYDARFLQ
                     TFETINAKQTRGQAVENKIPVPFGVLYREERSVYEKDLEAQVQAVIQKKGKGDIAKLL
                     RAAETWRVD"
     gene            complement(8698..10557)
                     /locus_tag="PLY93_01375"
     CDS             complement(8698..10557)
                     /locus_tag="PLY93_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRP68163.1"
                     /translation="MQPEIKSKIKQKIPSATVRFTGDSGDGMQLVGGQFSQITGIAGN
                     DVMTFPDFPAEIRAPQGTIAGVSGFQVHFGDHTIYTPGDKVDALIAMNPAAFKANLRD
                     LKERGVLIANSDEFNEKNLSKVDYQTNPLDAEMLQQKYHVIQVPIPASTQRALAESGL
                     SSKEIDRCKNFFALGITYWMYQRDVDKTKAWIDTQFAKKPHLASANKLVLDAGYSYAE
                     TTELFEVAYEITKAHFKPGTYRNITGNSALALGLVAATELADLPALYAGYPITPASDI
                     LHEIAKYKNYNIRTFQTEDEIAAIAAAVGASYGGSIGVTVSSGPGIALKSEAINLAVI
                     TELPVVIVDVQRAGPSTGMPTKNEQTDLLMALYGRNGESPVPVIAPSSASDCFYAAIE
                     ATRLAVQFMTPVFILSEGFLGNSSEPWQLPAVEDLEKIHVDKVKVGESRETYQAYGRN
                     EKTLARRWAPPGVAGFEHRIGGLEKDDNGNISYDPDNHDRMVRLRQAKIDRIADFIPE
                     LKAYPENKGDLLVLGWGSTYGAIREAVERVRKVGHNVAHAHLKHLNPFPKNIEQVLKS
                     FNKVLIPETNLGQLVLLIRGKYLIPAKSFNQVRGRPFGVDDLEAAILSTLKEK"
     gene            10714..11142
                     /gene="ndk"
                     /locus_tag="PLY93_01380"
     CDS             10714..11142
                     /gene="ndk"
                     /locus_tag="PLY93_01380"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRP68164.1"
                     /translation="MFEKETTLIMVKPDAVRSGYSGKILARIEEEGFKIRAMKRVRLS
                     LEQAQEFYSVHAARPFYGELCNFMASGPVVAAILERDNAVLHWRDVIGATDPAEAKEN
                     TIRKLFAKSKGENAVHGSDSVENAKIESAFFFSGLELAGQ"
     gene            11163..>11403
                     /locus_tag="PLY93_01385"
     CDS             11163..>11403
                     /locus_tag="PLY93_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRP68165.1"
                     /translation="MENLKSRFERDFFLLLKKSSLKSAPSLLREATHYALSGRNKRFR
                     PVLALEAARLTHLDDTRALCVAAAVECLHTYSLVHD"
BASE COUNT         3132 a         2624 c         2679 g         2968 t
ORIGIN      
        1 gaagcgacgt atcgcttgat tcccaaccgt aatatgttca atacgcgctt tctcgaccgc
       61 gtcatcgtca aaggcaagag cgagccgata ccgattctcg aaattctcga cggtaagaac
      121 gatatacgca ccgaaagaaa actgacacat cgagcgaaat ttgaagaagg cgcatatgca
      181 ttccatgcgg gcaaggctga cattgccttg aaaatttttc aagaattgag tgcggctcta
      241 atcgaagatc cagcgtgcga gctttatctt gaacgttgcc aactgcgtat cgccgggaaa
      301 aaaatagaag gtgtcgatga agagtataag aaaaaggcgt tgtgaatagt cgcacaaaaa
      361 tactcataac taaaatagtt tagtattttt tctttatgat aaacattatc gacggtaaaa
      421 ttaaagactt aggcgacggc tttcaagtgc gacgtatttt accccacgga agtttgcgca
      481 cggttggtcc ttttgttttt gtcgaccaca tgggacctgt taaaattgaa agagataaag
      541 cgctcactgt gcgcgcacac ccgcacattg gtctttcaac agtgacgtat ctttacgaag
      601 gcgtgataca ccacaaagac tcgataggca gcgacgtgct gatttatcca aacgaagtaa
      661 actggatgac cgctggccga ggcatcgtac acagcgaaca ttcacgactc gatcctcaat
      721 tcacaaactt gcatggcatg caagtttggg ttgcgttgcc caaggaatac gaagaagtaa
      781 atcccacttt tgaacactac gacgccgctg tattgcctga aataaaaaac gacgcgtaca
      841 ccttgcgcat tatcgcaggc tcattcatgg ggcaaagctc gccggtgaaa acacactcgc
      901 cgcttttcta tgccgactta cagttgaaaa aaaatattaa gatcccgtta gattttcccc
      961 gtggtcatga tgccgcactc tacgtcgcgc gcggtgaaat tcgtgtgtcg gggcaaagtg
     1021 ttctaaccgg cgcgatgatt gctcttacca aagatacgcc ctgtgaagtc gaagcaaccg
     1081 aagatgcgcg cattgcaatt ttgggcggtg agccattaaa agatccacgc catctctggt
     1141 ggaactttgt ttcaacctcg aaagagcgca ttgaagaggc gaaggaagcc tggcgtaaaa
     1201 atgcgatggg cgccgtcgcc ggtgaaaccg atagaatccc tttgccgtaa ccatcaaacg
     1261 attgacaaac tgacgccacc tgttctattt acactatgga aattaagcgt agtgaaatgc
     1321 ttcaatgggc gcaaagcgct caacttaacg ccgaatcgca caatgatgtg cagacagagc
     1381 gtgcgcatac cacttcggcg tcgcggcaca ccaccgtttc tatagacgcc ttacgcacaa
     1441 aagttttagg attgcaacaa gaagtgcacg atttacaaac cggtatttca tcgcgtcaaa
     1501 ttcaaatgag tttcttgaac caagtgggtg aaggctctgc atggcaaaaa gaactacgcc
     1561 ggtttatgaa cgaacaattt ccttcactct atttagagct caaagaaaat caaaatttag
     1621 atgaattcaa agctgaaacg actacttcca tcacccagtt acgtgaaaat ttattaaaaa
     1681 aagaaattca gattcaaaat attatttcag cgggtatttt tgaaccgaca caagggaacg
     1741 acaccgacga gatggcggcg gctaataatt cggaaatcat taaagattat acggcaagtc
     1801 gcgatatgtt tacaaaatta cgccccgata cagtaaaaaa tctactgcac taaatagcat
     1861 tgttgaaaaa taatgtaacg aggatcgatt caacgcttct caaagacgta ttgcgcgctt
     1921 gcgcgttgga atacaaaggc gaaggcgcac ccatagtaaa agcgtacgcc gaaggcgaag
     1981 cggcgcagcg tatgcttgaa attgcacgca agcacggcgt acaaatccaa gaagggcaag
     2041 aggagtcatt gcttttggct cttaaatccg taaaactaaa tactcaaata ccgaccgaaa
     2101 tttatctggc ggttgcgcgt atttatgcgt atcttatcgg aaagaaaaaa gagtaaaata
     2161 atagattatg cttgaattta cacaaggaaa atccgaaaat aacttatgac ccgctatcag
     2221 gtcaaactat tacgcccggg tgtttcgttt acgaaaccag tctatatcga cccgacaaat
     2281 atgctcgcgg cggcaaacgt catcgttact caaagcgata tcgatcgcct cgtaaaatgg
     2341 aaaattgaag aagtgctttc cgagggcgag cccgtctatg cgaacaagaa agacgaaagt
     2401 gctttcatgg acggccagca gaaaattgat attgagaata ttaaacgaga acttaaaaat
     2461 ttgctacgtg ccaagcctgg gtttaataat cttaccgaag cctccgaagc gatactaaag
     2521 aatttttacg aacgggtaaa tcagaacaat aaccccgtga cgaacgatgt gcgtaacaaa
     2581 gcggaagaga ttaccaactt tgtcaccgat aaccctcttt tggtgattta tgtgcttcaa
     2641 tatattattg aaacaagcac gtataaacac gtcatcgctt gcgctattta ctctgtgcgt
     2701 ttaggtcttg cgttggattt ctcacgtcct aaaattgtcg aacttgtttt tgcaatacta
     2761 atgatggatg tcggtatgct caaagtaccc tcgacaatta ttgaaaaaaa agagaaactg
     2821 aacgaaaccg aattacaaat tatacacgcg cacacggtgc acggctatca aatgctaacg
     2881 caaaccgcga aaattaaaaa ttcgattgca tcggttgcgt tgcaacacca cgagcatttt
     2941 gacggctctg gctatccgcg caaaattaaa ggcgctgaaa tggcagagta cgcgcgagtg
     3001 gcctcaattt tagattcgtt ttgcgccatt atttcagaaa agccgtaccg caagaaccgc
     3061 ctcccgtacg aagcgatgaa agaactattg actttaggta tttatcgtta cgatcccatt
     3121 tatctaaaaa cattcctgga ttcgcaggcg atatatccct taggttcgat tgttgaactc
     3181 tctgatggtt cgacaggaat ggtagtgatg gcaagtaaag gtaaaccgat gcgccccgtc
     3241 gtttggattt tacgcgatgc gaaaaaccaa cgccccaaac aactggagtt cgtacatttg
     3301 ctctacacta ccgataaatt tatatcgagg gccaagtacc cgcaagatgc agacttgaac
     3361 ctcgatttgg aatttactca gctatcgaaa ctactctaag ttattttttt gattttttgg
     3421 gtttatcttt tgcaaacaac cccaactcgc taaaactggt gagcaccttg tccgccaaac
     3481 catattcaac cgccccttta ccgtcgagat aataatcgcg gtctgtgtct ttttcaatgc
     3541 gcgacaaagg ctggccagtg gtttcggcga tgatgcggtt gagttcctcc cgcgtttttt
     3601 taatttcttc ggcttgaatt ttgatatcga gagccggtgc aacaatttgc ccactgatga
     3661 gcggttggtg gatcattact tttgcgtgtt gaaacacata acggtgtttt ttttcaccca
     3721 ccgttaaaag aatcgctccc atcgacgccg ccatccccat ggtgatagta tataccggcg
     3781 atgaaatcat tttcataaca tcgtaaatcg ccatacccga tgtgatcact ccacctggtg
     3841 agttgatgaa aaagtaaatg ggctttcccg gatcgttcga ttcaagaaaa agtaaacgct
     3901 cgacaatcga ctgcgccgaa cgatcagtga caggccccca caaaaaaatt tggcggtttt
     3961 ctaagaagcg ttcggcaagg cgctcaccca gcggcgctgc gccttcactc tctttatctt
     4021 tttttttctt tttgtcatcg tcgtcatcat cgtcatcaaa acggaaagga attgtatttg
     4081 tgaatttctc aaatggattt tgaatcatag aatcaatata ttattttaac cgtaacatgg
     4141 acattatttt attgaagcgc ttgtggggat agttaccccc taatacgaca taatacagaa
     4201 aaactaatga cactaatgtc attttttaat tgacgcggca tgttgaggga taactatccg
     4261 tgcatggaaa acctaaaaaa taaagtcgtg ctcatcaccg gcggtgcggg cggtattggc
     4321 ctcaacacgg ctgcattttt tgccgaggct ggttgtcggc tggtgctcac cgacctcaac
     4381 gaagctgcgc tcgtcaacgc cgagcggcgg cttgcgaaac ataatgtact tattttaaca
     4441 caggcaatgg acgctaccga tttgaaggct gtggaaacat tgcgagacaa gattgaattt
     4501 cagtttggtg gtgtggatat tttagtgaat aacgcaggca tcggctttaa cgcggagatt
     4561 gccgacacat ccattacaaa atggcggcaa ctgatggatt tacacttctg ggcgccgctc
     4621 tatcatatcc aaacgttttt gccagcgatg aaggcgcgta agagcgggca cattgtcaac
     4681 gtgtcgagcg gccaggcgta ttttcgtgcg ccgacgtggg gcgcttattc agtcaccaag
     4741 cttgcgattg gtgccatgtc tgaaatttta tcggtggaag tggcgcgcga caacgtacac
     4801 gtttcgacgg tgtatccttt cctggtcaat accggttttt acaacacggc gagtgcaagc
     4861 tctttcggag aaaagctttt tttacaattt ttaccgctct attcgcacaa gcccgaagaa
     4921 gtcgcagcac ttatattcaa cgcagtacgc gaaaataaaa ccgttgaaat ggtaagcgta
     4981 ttcaaccagt ttttcaaata catgagagtg gttaatcccc ttgcgagtgt catcgactgg
     5041 atgacgctca ccttcatgtc aaaaaagcaa caagacgcgt ttggattttc ttcgcttctg
     5101 gagcgatgga ctgaaacgat gaactcaatc cttgtaaata ttaaaaacca cgcgcctaaa
     5161 aaaggattta tcatccacga ggtgatgacc ggtgagcatg agttcaccga agggtttggc
     5221 ggtaaaggta agcacgagtt tttctttgag gccgattggg gagtcgacaa tctcatcgat
     5281 tttttaaaca tcgccgacaa cagcaaattt ttagtgagca cccttcgggg cacggtgacc
     5341 gttggaggct tttgccaaaa tgtaccgatg caaggcacga ttgagctgcg ttacctacaa
     5401 gagcagaaaa tacgatacac gtttgaattc gaggtatcgg gcgaacgcta ccgttacatc
     5461 ggcgaaaaac gcgatattta tccatggaat ttgctttatt cgcacaccac ctgctttgga
     5521 acagtgacta aactcagcac gggagaagtt gtgtctaatt cggttactca ctttcttatg
     5581 gaaagtctac cacaattttt gggctcgcta tcaataacgg gataaattta gagtctgtgc
     5641 gccaactcgc tgagacaatt tttttgcaaa ttttttacgc gctgcgcgag ttggcgcata
     5701 ggctctttac tgagtcgacg gtcaaccgct aaaaatgctt gtcctgtgta cttcatactc
     5761 tatcgttgtc catgagtata tggcaacgat ttaagcgcgc aatgcgctct atttttggtg
     5821 gatgggtttc cgaaatggaa gatccttctt tgattcttga acaaaacatc cgcgacatgc
     5881 gcgataaaat tcctaaaatg aacgaaaata tcgcgatgtt aaaagcaaac gcaaaattac
     5941 ttgagaagga aaagcaaaag cttttgagtg aacaagaaaa gttagttgca aacgtgaaag
     6001 ctgcaatcgg tcaaaaccgc gaagacattg ccggcgaata tgcgttacaa ctcgaacaaa
     6061 acaaaacaaa ggtacagcag gttgaacaaa acttagcgca agcgcagacc gctgccgtca
     6121 aaggagaaga ggcaaaggta tcgtttatga aagaactcga ccgcaaaacc aaagaagctc
     6181 tcgacgcgat tgaatcggcg aagcgtgcga aatggcaaaa agaagtcgct gacaccatgg
     6241 agcaatttac cgctggccgc gtcgaccata cgcacgacga aatggtaaag cggattgaac
     6301 gcgacggagc gatggccgat gcaaaactcg atatggccct caacaaagaa gataaaggtc
     6361 gtgcaaaaat cgaagccgac gccgaaaaaa tccgcgcggc agaacttgtt aagcaattca
     6421 aagtcgaaat gggggtagcg ggtacatccg caccagcgtc gacaacttca gaaggtggat
     6481 cccaagagca agcgggagtg cggaactaaa ttttttactt caatccgcaa cgcgggctga
     6541 agtaaaacta caacaatgtt tgcaattctc taaaagccgc cgataagaga aagggtcgcg
     6601 ctgtgggcga ctcttatcca gagaacgagc ccggcatttc aattgtcgaa atgcatttat
     6661 cgaagacaga attttataca caagcgcaac ttccagtata tccaacttta ttgcgacacc
     6721 atttgcaggt aaaagaatgg cgctcagccg tatttttgcc taactaaaac ggtctaaaca
     6781 tagcgcagcg gtcaccgaca cattcagcga ttctatccgt cctgaacccc ggatactaaa
     6841 atggccatgg cactgcgcta caaataaaga agggagtccc tcttcttcgt tacccaaaca
     6901 gaccaccaaa ggggatgtct ttttatcgcg caagaattct tcgagcgtgc gctgtgtttt
     6961 tgctgtcggc ttgaccgcag cgagcgcgcg atagtttttc tctttcgccc acgtaaaaaa
     7021 ttcttcgtcg ttgtcgtaca cccaaagctt gacgtgcgtt aatcccccct cggcgacacg
     7081 ccacgcggca gcggtttcag ccgcaacgta agttgaacgg ctcacacaaa ttctttcaac
     7141 accaaaaaac gccgccgtcc gcagtatcgc tcccacatta tgcggatttt ccacgttatg
     7201 caaacaaagc gtatggtgat tcagtatatc caactttttc ggttttgttt ttgtgatgac
     7261 agcagccaca ccttggtggt gcgccgtcac tgcaatgcgt ttgagctcgt cggtggtaac
     7321 catgcgatat attttgtgcg tttccgcgag atacttacac gtctgcgcaa aaagtggcgc
     7381 ggaagcttcg tcaaaaaata gccgttggat gcgttcgggt gtgtgttgtg cgattgcttt
     7441 tacactcgaa aagccgcaga cggtgagaag attttccgac atgggttgat ttgtattttt
     7501 gagagtgggc tgcatgagaa ttttctatgt ttgcgcacaa gtaggatcgt ttttcctcaa
     7561 attttgctca tttttgacta ctacagcact cgggggaata gttaccccca aatgcgacat
     7621 aatacgatag gcaaccaata aaaaggttca atcgacacgc caagtttctg ctgcgcgtaa
     7681 gagttttgca atgtcgccct tccctttttt ttgaatgacc gcttgcactt gggcttctag
     7741 gtctttttca tacaccgaac gctcttcgcg gtataacacg ccaaatggca cggggatttt
     7801 attttccaca gcttgcccgc gcgtttgttt tgcattaatg gtttcaaatg tctgcaaaaa
     7861 acgtgcgtcg tacgcgtcga aggtggaaac tgcgttgggg ttatcgacag ccgaaacaac
     7921 atcaaaagtg cctttttcat tgaaatgcaa ccctttttct ttttcagcgc caaagagaag
     7981 tttttcgccc tgctcgacaa aaatcatggt gttggcacgg ttttctttac cgacgatggg
     8041 ttcaaaaacc tcgtcattaa aaatcacaca gttctgcaaa atttcaacta aagaagtacc
     8101 tgcatggttg tgtgattcct tgaagatttt ctccatgtgc ttcatgtctt tgtcgtatgt
     8161 gcgcgctaag aatgtgccgc ccgcgccttg tgcaagccgt aacgccgaaa aaggcgtgtc
     8221 gatagaacct tcgggggtcg ttttagcaat cgtaccacgt gcgctcgtgg gtgagtattg
     8281 gcctttcgtg agcccgtaga tttcattatt aaagaggaga atattgatgt cgatattgcg
     8341 acgcaggacg tggatgagat gattgccccc aatggaaagc gaatcaccgt cgccagtgat
     8401 gacccacacc gaaagctcgg ggcgtgatat tttaagcccc gatgcaatcg ccggtgcacg
     8461 cccgtgtatc gagtggaaac catacgtttc catgtaatac ggaaagcgcg acgagcaacc
     8521 aatccctgaa ataaaaacaa tgttttcttt ggagacacca atctccggca tcgttttttg
     8581 cactgcggtt aaaataccat agtcaccaca acccgggcac caacgtaccg tttggttcga
     8641 tgtaaaatct ttttttgtga gtgttgctgt agctgtcata gttattccta attttcctta
     8701 tttctcttta agggtcgaaa ggatcgccgc ttcaagatcg tcgacaccaa aagggcgccc
     8761 tctgacttgg ttgaaacttt ttgcgggtat aagatacttc ccccgaatga gtaggacgag
     8821 ttgtcccaag tttgtttcgg ggataagcac tttgttaaac gattttaaca cttgctcgat
     8881 atttttagga aaggggttta agtgtttgag gtgtgcatgc gcgacattat gtcctacttt
     8941 acgcactcgt tccaccgctt cgcggatagc gccgtacgtg gagccccaac cgagcacgag
     9001 caaatcgcct ttgttttctg gataggcttt aagttctggg ataaaatcgg cgatacggtc
     9061 aatttttgct tggcgcaacc gtaccatgcg atcgtggttg tcgggatcgt aactgatgtt
     9121 accgttgtcg tctttttcaa ggccaccgat gcggtgttca aaacctgcga ctccaggagg
     9181 agcccaacgc cgtgccaatg ttttttcgtt gcggccatac gcctggtaag tttcgcgcga
     9241 ctcgcccact ttcactttgt cgacatgaat tttttccaaa tcttcaacgg cgggcaactg
     9301 ccacggttcg gagctattcc ctaaaaaacc ttcggacaaa ataaagaccg gcgtcataaa
     9361 ctgcaccgca agtcgtgtgg cttcaatcgc tgcgtaaaaa caatccgacg ctgacgaagg
     9421 tgcaatcacc ggtacggggc tttcgccgtt gcggccataa agagccatga gtaaatcggt
     9481 ttgttcgttt ttggtaggca tccccgtcga tggccctgca cgctgcacat cgacaatcac
     9541 caccggcaat tcggtgatga ccgccaagtt aatcgcttcc gatttgagtg caatccctgg
     9601 ccctgaggaa acggtgacgc caattgaacc gccataagat gcgcccactg cggcggcaat
     9661 cgcggcgatt tcatcttcgg tttggaaagt acggatgtta taatttttgt attttgcaat
     9721 ttcgtgcaaa atatccgacg caggcgtaat gggatacccc gcgtagagcg ccggtaaatc
     9781 ggcaagttcg gtcgcagcga caagacccaa cgcgagcgcc gaattaccgg tgatgttgcg
     9841 gtaggtgccg ggcttaaagt gcgctttggt aatctcatac gccacttcaa aaagttcggt
     9901 ggtttcggca taactatacc ccgcatcgag gacgagttta tttgcgctgg ctaaatgtgg
     9961 tttttttgca aattgggtgt cgatccacgc tttggtttta tctacatcgc gctggtacat
    10021 ccaatatgtg atgccgagcg cgaaaaagtt tttgcatcga tcgatttctt tggaggaaag
    10081 ccccgattca gccagagcac gctgcgtcga tgcagggatg ggcacctgaa tgacgtggta
    10141 tttttgctgg agcatttctg catcgagcgg attggtttgg taatccactt tagataaatt
    10201 cttttcgttg aactcgtcgc tattggcgat aagcaccccg cgttctttga gatctctgag
    10261 gttggctttg aatgcagcag ggttcatggc aatgagcgca tcgactttgt cgcccggggt
    10321 gtaaatggtg tggtcgccaa agtgtacttg aaaacccgac acaccggcga tggtaccttg
    10381 tggcgcacgg atttctgcgg ggaagtcggg gaatgtcatc acgtcgttac cagcaatgcc
    10441 ggtaatttgt gaaaactggc cgccgacgag ttgcataccg tcgccggagt cgccggtgaa
    10501 acgcaccgtc gcgctgggaa ttttttgttt tattttagat tttatttctg gctgcatgtg
    10561 ccctcgagga ttgaattgtg cgcagacttt ttctattcag tgcgccgcaa agtactttat
    10621 ggaaattaag gtctgaaatt aagagtcaac tgaactttgc atagagccat gtcgtaacgt
    10681 ttgacgcggt gtctaaaaaa gcaaatttac cctatgtttg aaaaagaaac gacgttgatt
    10741 atggtgaaac ccgacgcggt acgctcgggc tattcgggca aaattttagc acgcatcgaa
    10801 gaagaaggct tcaaaatccg cgcgatgaaa cgtgtgcgtt taagcctcga acaagcgcaa
    10861 gaattttata gcgtgcatgc agcgcgcccg ttctacggtg agctttgcaa ttttatggca
    10921 tccggccccg tggttgcagc cattttagag cgcgacaacg ccgtcttgca ttggcgcgat
    10981 gtgatcggcg cgaccgaccc tgccgaagct aaagaaaata ccatccgcaa actttttgca
    11041 aaatcgaaag gcgaaaacgc agtgcatggt tcagactcgg tagaaaatgc taaaattgaa
    11101 tcggcatttt tctttagcgg tttagagctc gcaggtcaat aaaattcgtc ttgttggatt
    11161 tattggaaaa tctgaaatcc cgcttcgagc gggatttttt tttgttactc aaaaaatcat
    11221 cgctcaaaag cgctccatcg cttttacgtg aagcaacaca ctacgcgctt tcggggcgca
    11281 acaaacgctt ccgccccgtc cttgctttag aagccgcacg cctcacgcac ctcgacgata
    11341 cgcgcgcgct ttgtgtcgct gccgctgtcg aatgcttaca tacctactcg ttggtacacg
    11401 acg
//
LOCUS       DAOMPD010000019        11318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2474_length_11318_cov_2.835213, whole genome shotgun sequence.
ACCESSION   DAOMPD010000019 DAOMPD010000000
VERSION     DAOMPD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_11318_cov_2.835213"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="PLY93_01390"
     CDS             complement(<1..1587)
                     /locus_tag="PLY93_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRP68166.1"
                     /translation="MKFRRPFFQFFLSFFHRADSAFNHLFTEKGNFLYYLGALPIVLL
                     FVLLITGLFLFIYYNMSVHQSYDSVKYMTESALFGRFIRNLHRYAADAMMFFVLLHLA
                     RMLFEEKFQNHRTLAWVSGLIILFFMLMQGITGYILPLDANSRYVMEKTSELLAGLRV
                     FGDTLPRSFSSPALLGKWIMWVIMVMHLVVPLFFVLLIFIHTLRVTRAKLFPPRKVTI
                     AFLVVLSLFTIIFPISMVEKAAAEKMPELLQPDWFFLFFAAVFDTQWSWVAWAGMGTF
                     FLGLLALPWLLKKRKIITAGVIADNCTGCGLCAIDCPYQAMHMVERKSEAGQAIVQAQ
                     LPDGTPTEASQAADKFKYIVSINTDLCSGCGVCAGSCAYDAITHVSPGEKHEKIDWTP
                     AKDKWVTVVCQGRAHSLGLEDDGSVLRPYHDTLLHISPCTGKMGVSLTQDIEAAGAKG
                     VIVGACPEGDCWYREGNKWLMDRINIQRKPNFRKISPEFPILGHRFNGRQHASFTREI
                     GRIVAGDGSLVKRVFAFLKPS"
     gene            complement(1700..1834)
                     /locus_tag="PLY93_01395"
     CDS             complement(1700..1834)
                     /locus_tag="PLY93_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68167.1"
                     /translation="MSENNQKEKMHAPGSFVIALIFLAWFMTVYFTQWLALSKNWTVY
                     "
     gene            complement(1849..3411)
                     /locus_tag="PLY93_01400"
     CDS             complement(1849..3411)
                     /locus_tag="PLY93_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP68168.1"
                     /translation="MDLSIISNQFRTCPATGLKVHLPAQRLVWINAVSAVVFLLLGGV
                     LALLLALTRWPAVHLLPQDLFYRFITAHGVSMLVFWIVFFEMAGIVFAVQTILGARYP
                     GIKFAWFNYIMMLVGAIMTEVTIFMGKADVTFTAYPPLKAHPAFYLGIILFAVGALFN
                     CFQFIYALIVAKREGWHQGTLPLFVFGMLVAVIIAIFTLVSGAAAFIPAFLWSIGLIG
                     YVDPEYYRITFWGFGHSAQQLNLAAMVSIWYLLANLTTGAKPVNQRMCRLAFVLYLFG
                     INLGSIHHNLVDPGLSSTYRIFNTSYLFYAATLGSMIHAFSIPAAVEMAQRKKGFTRG
                     LFQWITNAPWSNPGFSGMVLSLVIFGFFGGPTGLILSVEQLNMLSHNNLRVPGHFHMT
                     VVGGTTLAFMAVTYYVIPLVLQRKLVGEKLAKWQPYVFGLGVLMFTTGMHFAGVMGAP
                     RRHWDVTFQNAVIQVPFDPAMYTMLALVGIGAIVAFTGLFMYIYIAVASVFFGKKAET
                     KMEPYMPPFAKN"
     gene            complement(3413..3991)
                     /locus_tag="PLY93_01405"
     CDS             complement(3413..3991)
                     /locus_tag="PLY93_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68169.1"
                     /translation="MPLETPKKNWWEPFNKEERIWLWISIAWCIIMFIMMPLGHLWNQ
                     NVSSETYKTNPEEYAKVVDNFIKKYERKDAQGKVVTHNGKAGGIPLVEAPTKDQGDTF
                     LVAQAWQFRPALVLKKNKTYRIHMSSLDYQHGFSLQPQNLNIQILPDYSFVITMSPNE
                     TGKFFLICNEYCMFAGERMGHDSMVGQVIVED"
     gene            complement(4001..4249)
                     /locus_tag="PLY93_01410"
     CDS             complement(4001..4249)
                     /locus_tag="PLY93_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68170.1"
                     /translation="MQEEQPAKRTLGQKMFDNVWFLLFVGMAFPGVFYLGWGLLEIFH
                     FNNVQLKDYLAKTNQTYLLQAEQPAAPAEVSKEAPEKK"
     gene            complement(4385..5032)
                     /locus_tag="PLY93_01415"
     CDS             complement(4385..5032)
                     /locus_tag="PLY93_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRP68171.1"
                     /translation="MIKKGQHKENNFISFWKAMPSELEGRLRKAAVHIHFKNGEHVFR
                     ENDKYRGVFFVESGYFKWYMLGEDRHSVVIKIFEAGEIAGLPPLFDAPPQQYIANLVS
                     IGKGSLFFWPASTFKQLIRIYPDYIFVFNRYITATVKQLALAKAAVTSKSVDMRVEDY
                     LQSLGASDAWVSLPMPKRQIAFALNTSAETFSRALARLQKQGRLLHNSGSYRLSV"
     gene            5200..5811
                     /locus_tag="PLY93_01420"
     CDS             5200..5811
                     /locus_tag="PLY93_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRP68172.1"
                     /translation="MTSINFAKLNSQKTELLQNISHYKRFAKNENVFEEGDVYRGPYI
                     VVEGQFKVFMLGDAGKESIMHVFREGELLAGGPLFLGGTYPASCASLSDGCLIAFEYD
                     RLKKIIDTDESIHNYFLQRTLKLLPRLKQKIESLALKSAEQRIINYFKSLGADRAPVA
                     LDIPKNQIAALLDLTPESVSRVLNQLIQREILSVDEKTYSLKK"
     gene            5961..6386
                     /locus_tag="PLY93_01425"
     CDS             5961..6386
                     /locus_tag="PLY93_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system-associated lipoprotein"
                     /protein_id="HRP68173.1"
                     /translation="MLKQTLFSLATALILLSCSSRFVKKEDVQEFSKEYEKTYVLKKN
                     IEVGNFETLNEGAKVRVYFKTAGDYIAVYAYPYSQKREEAVGKNILQVFDADFPDKKF
                     NPQVIRDKLAPLIAEEDAVKTEKKSTKTKKEKKKNKGKK"
     gene            6388..7647
                     /locus_tag="PLY93_01430"
     CDS             6388..7647
                     /locus_tag="PLY93_01430"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRP68174.1"
                     /translation="MKIGIFGLGTVGQGLVDIIRQKKSDLQIAAVVDRSFAKKEQILQ
                     GIPASADKDFILKDESIDTVVELMGGIDDSLYIVRSAIEAGKNVVTANKALLAEHGYS
                     LFHLAHRMADKGSPRILYEAAVAGAIPIIRNIDTIFRNEQITRLMGIINGTTNYILTR
                     MRKESLNYGDVLEAAQKQGLAEADPTLDVGGYDAQQKLALLLSLITGKWVEAKNIPVR
                     GITELSSQDVVWAERFQNRIRLVADYTYDNGQHYMHVTPALVDERNVLYDIEYENNAI
                     LFHGEYSGEHLFMGKGAGKYPTAFSVYSDLLTLKSQAGCALPQRELSTNYLAIGDIAD
                     TSSSFYLRLRVKDQPGVLAAVAQILGNNALSIASVHQDHSAVAAGEVDIVIHTHSAKR
                     SGFYKALQEIKNLKNFVLHQVYLPVLR"
     gene            complement(7660..8565)
                     /locus_tag="PLY93_01435"
     CDS             complement(7660..8565)
                     /locus_tag="PLY93_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRP68175.1"
                     /translation="MPLRSHNILALLIEIGKDLANNKNLATSQAFMQLIATEASWAEI
                     FQNIDAVPDFALRHKLRTLEIDYTTAFVYNQLGHVLPRSNNDVYAIERISYLTGLLAT
                     PFKLWDEYCAALDSLEVDLRQRLESVHLTPRRVKGRFDDLKIMHEILSALNETIFEKN
                     GISGSDNAINCTEAYSPQAVLCERIPGIPLSIAMIYLIAGLRLGFPIYGVNTPARFLV
                     KWQYGNLELFINVFDRGTVIDRDKLDQLMRENFPNYSNRLLDAVPFEIIARRAIANTA
                     ALAARNEDYDRSRKLEALSRTLFGF"
     gene            complement(8589..8918)
                     /locus_tag="PLY93_01440"
     CDS             complement(8589..8918)
                     /locus_tag="PLY93_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68176.1"
                     /translation="MQFPEIIWYVIIPTAAITSIVTAALVYFLARKLYYDEIEKPIDD
                     LPDRISLKVEEAGDRLLPRFEERVKKGFLAALKDSPKLGPEMTAGMIESALKGMDQII
                     TGRKKRE"
     gene            complement(8987..>9595)
                     /locus_tag="PLY93_01445"
     CDS             complement(8987..>9595)
                     /locus_tag="PLY93_01445"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HRP68177.1"
                     /translation="YTARLTSALGTTYETVWSVLGDRLFFKTCKEYIASHKSISYNLN
                     NYGATFPAFLRGKFKQFTFLGDLAASEFAFQQLFNTAPVVPSVLNFQSIHPERTQFQF
                     FETASLWSFTHRLYPLWRNRAKIPHGYIPRNFATAEFILMYKRHGEILIELLTENQYK
                     IAKALLKKKSLNRVLNTSKAKPKEVERLFQVLSNGGVVSKIR"
     assembly_gap    9596..9605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9606..10638)
                     /locus_tag="PLY93_01450"
     CDS             complement(<9606..10638)
                     /locus_tag="PLY93_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HRP68178.1"
                     /translation="MLGVGLRAKHFPYLAKHKANVDFFEVLSENYIDTEGYPREVVRR
                     LRRDYKMCMHGVSLSMGSATGVNNAYLKKLKQLADEIEPFLISDHLCFTGVKRENSHD
                     LLPLPFTMRMVDIVVRNIDTVQNFLKTRIALENVSSYLTWRASEMSEWDFINTIVQKS
                     GCKILLDINNVYVNAKNHGYNADDFLTRINPTHVGEIHIAGYSDMGTHLFDTHAEPVH
                     APVLELWKKHAARFHKIPMIVERDDAIPAFHVVEREVLRLGKIRENAYIKERKNRIVM
                     EVFLPPRVPEKRNNADVGKLQKVFLEGIQNQNFASLAQVKGGGKLSDKAALKVYHAGW
                     YIHTGRSFIF"
     gene            complement(10641..>11318)
                     /locus_tag="PLY93_01455"
     CDS             complement(10641..>11318)
                     /locus_tag="PLY93_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68179.1"
                     /translation="TRENVFRESLAPEIRFFRVRSIHKTGVAGAYSPTYPIDAYLAKP
                     YKEPSIPVVQQGQTEYLLGNRIELPTQPNLVTKYKLGDGEFIEYREPIVFEKPATYKM
                     VVNLENQNQEVVYTKNFVFKVELNPPVTRAILSNPVHSSRGITFGKESALLFLVNERE
                     SGLAKTFYRVLPLGKDLASVSTSEYKNRLTHADICTPEKITLVEFYSTDKAGNKEQPK
                     SQVFFCE"
BASE COUNT         3131 a         2618 c         2607 g         2952 t
ORIGIN      
        1 cgaaggcttt aagaaagcaa acacgcgttt gacaagagaa ccatcccccg cgacgatgcg
       61 tccgatttca cgcgtgaacg acgcgtgctg tctaccgttg aaacgatgcc caagaatggg
      121 gaactcgggg ctgatttttc taaagttggg tttgcgctgg atgttgatgc gatccataag
      181 ccacttgtta ccttcgcggt accaacagtc gccttcgggg caggcaccca cgatgacgcc
      241 ttttgctcct gccgcttcga tgtcttgcgt taaactcacg cccatttttc cggtacacgg
      301 cgaaatgtgg agcagggtat cgtggtaagg gcgcaataca ctgccatcgt cttcaagtcc
      361 caacgagtgc gcacggcctt ggcacacgac agtcacccat ttatctttag caggtgtcca
      421 atcaattttt tcgtgttttt cgcccgggct tacatgcgtg atggcgtcgt acgcgcacga
      481 accggcgcac acaccacagc ccgagcaaag gtcggtgttg atggacacga tgtacttaaa
      541 tttatccgcc gcttgcgatg cctcggtggg tgtgccgtcg gggagttgtg cctgcacgat
      601 ggcttgaccc gcttccgatt tccgttcgac catgtgcatc gcttggtacg ggcagtcgat
      661 ggcgcaaagg ccgcaaccgg tacagttgtc agcaatgacg ccagcggtga taattttacg
      721 ttttttgaga agccacggca atgcaagcaa ccctaaaaag aatgtcccca tgcccgccca
      781 tgcgacccag ctccattgag tgtcgaagac agccgcaaag aacaaaaaga accagtcggg
      841 ctgcaacaac tctggcattt tttctgccgc tgctttttca accattgaaa tcgggaaaat
      901 gatcgtgaat aacgataaca cgacgagaaa ggcaatcgtc actttgcgtg gtggaaaaag
      961 ttttgcgcgt gtaactcgca acgtgtggat gaagataagc aatacaaaga aaagaggcac
     1021 cacgaggtgc atcaccataa tcacccacat aatccatttg cccaagagcg cgggagaaga
     1081 aaatgaacgc ggtaatgtat cgccaaaaac acgtaatccg gcaagcagct cggaggtttt
     1141 ttccatgacg tagcgcgaat tggcatcgag cggcagaatg taaccggtga taccttgcat
     1201 aagcataaaa aataagatga tcaacccgct cacccacgcg agagtgcggt ggttttggaa
     1261 tttttcttca aaaagcatcc gcgccaagtg tagtaacaca aaaaacatca tcgcatcggc
     1321 ggcgtaacga tggagattac ggataaatcg accaaagagc gcactttcgg tcatgtactt
     1381 gaccgaatcg tagctttggt gtaccgacat gttatagtaa atgaataaaa aaaggccggt
     1441 gataagtagc acaaaaagga gcacaatcgg cagcgcgcct aaatagtaca aaaaattacc
     1501 tttttcggta aagaggtgat taaaagccga atcggcacga tgaaaaaagg aaaggaaaaa
     1561 ttgaaaaaaa gggcgacgaa atttcattaa aactccaaac tgggggcgga aaaaagcgga
     1621 accccattta gtctgtgcgc tttaatattt tttttaatta gtaaacgcac agacccttat
     1681 gggttcagtc tttttattct taatagactg tccagttctt gctgagtgca agccactggg
     1741 tgaaatacac ggtcataaac catgccagaa agatgagcgc gatgacaaaa ctgccaggcg
     1801 cgtgcatctt ttctttttga ttattttcac tcatagtttc ctcccgtttt aatttttagc
     1861 aaaaggcggc atgtacggtt ccattttcgt ttcggctttt ttaccaaaga aaacggacgc
     1921 taccgcaatg taaatataca taaacaatcc ggtaaacgct acaatcgcac cgataccgac
     1981 taatgcaagc atggtgtaca tcgcagggtc aaaagggact tggatcactg cattctggaa
     2041 tgtaacatcc cagtggcggc ggggagcgcc catcacaccg gcaaagtgca tacctgtggt
     2101 gaacatgagc acgcctaaac caaaaacata cggttgccat tttgcgagtt tttcgccgac
     2161 gagcttacgc tgcaacacga gcggaatcac atagtacgta accgccatga acgccaacgt
     2221 ggtaccgccc accacagtca tgtgaaagtg accaggcacc cgtaagttgt tgtgcgaaag
     2281 catgttaagc tgctctactg agagaatgag accagtgggg ccaccaaaga aaccaaaaat
     2341 cacaagtgaa agcaccattc ctgagaaacc agggttgctc cacggagcat tggtaatcca
     2401 ctggaaaagt ccgcgtgtga aacctttttt ccgttgcgcc atttcgaccg cagcaggaat
     2461 cgagaatgca tggatcatcg aacctaacgt cgctgcatag aaaaggtagc tggtgttaaa
     2521 aatacggtat gtggacgaaa gtcccgggtc gaccaagttg tggtggattg aacctaagtt
     2581 gataccgaaa aggtagagta cgaacgctaa acggcacatt ctttggttta ccggttttgc
     2641 gcctgtggtg aggtttgcga gcaaatacca gatggacacc attgccgcca agttgagctg
     2701 ctgcgctgag tgaccaaatc cccagaaggt gatacggtag tattcaggat ccacatagcc
     2761 aataagacca atcgaccaca agaatgcagg gataaacgcc gctgcacctg aaaccaacgt
     2821 aaagatggcg ataatcaccg caacaagcat accaaataca aagaggggta atgttccttg
     2881 atgccaacct tcgcgttttg ccacaatcag tgcatagatg aactgaaagc agttaaaaag
     2941 cgcacccact gcaaagagga taatccccaa gtagaaagca gggtgtgctt taagcggtgg
     3001 atatgcagtg aaagtaacgt ccgctttacc cataaaaatg gtgacttcgg tcattattgc
     3061 accgacaagc atcatgatat agttaaacca cgcaaattta atgccaggat aacgcgcgcc
     3121 taaaatggtt tgcacagcga aaacaatgcc tgccatctca aaaaacacaa tccaaaacac
     3181 tagcatactt acaccgtgag cggtgataaa tcggtagaat aaatcttggg gtaaaaggtg
     3241 cactgctggc cagcgggtta acgctaaaag caaagcaaga actcccccta agagtaagaa
     3301 aacgacggct gaaaccgcgt taatccatac gagtcgctgc gctggcaggt gaactttgag
     3361 tccggttgcc gggcaggttc taaactgatt tgatattata gatagatcca tgttaatcct
     3421 ccactatgac ttgacccacc atggagtcgt ggcccattct ttcgcccgca aacatgcaat
     3481 actcgttaca gataaggaag aattttcccg tttcgttcgg gctcatcgtg atgacgaagc
     3541 tatagtcggg caaaatttgg atgttcaaat tttgtggctg tagtgaaaac ccatgttgat
     3601 aatcgagtga cgacatgtga atgcggtatg tcttattttt tttgagcacg agcgccgggc
     3661 gaaattgcca tgcctgtgct acgaggaagg tatccccttg gtcttttgtc ggcgcttcaa
     3721 cgagcggtat accacctgct ttaccgttgt gcgtaacgac tttaccttgc gcatctttgc
     3781 gttcatactt cttaatgaag ttgtccacaa cctttgcgta ttcttcgggg tttgttttat
     3841 acgtttctga cgagacgttt tggttccaca gatgccctaa aggcatcatg atgaacatga
     3901 tgatacacca tgctatggaa atccatagcc aaatacgctc ttctttgttg aatggttccc
     3961 accaattctt tttgggcgtt tctaaaggca ttttttctcc ttattttttt tccggcgctt
     4021 cttttgatac ttccgccggt gccgccggct gttctgcttg cagtaaatat gtctggtttg
     4081 tttttgccag ataatctttt aactgcacgt tgttgaagtg aaaaatctcg agcagacccc
     4141 aaccgagata gaaaactccc ggaaacgcca tgcccacgaa caacaagaac cagacgttat
     4201 caaacatttt ctggcccaga gtccgctttg caggttgttc ttcttgcatt accttcctcc
     4261 ataaaattaa ggatgagcta ctcaccactt atgcggtgga tagtcaagct taactttgat
     4321 tgctattgcc ttatagggtg ctctaaaaaa ttgctttgat ttacatcaat ttgggacgaa
     4381 ttttttagac gcttaatcga taactgccgc tgttgtgcaa aagtcgtccc tgcttttgga
     4441 gtcgtgccaa tgcacggcta aatgtttccg ccgatgtatt gagcgcgaac gcaatctgcc
     4501 gcttaggcat aggtaaactt acccacgcat ccgatgcccc caacgactgg agataatcct
     4561 ctacgcgcat atcgacgctc ttggatgtca ccgctgcttt tgccaacgca agttgtttca
     4621 ctgtcgcggt gatataacga ttgaacacga aaatatagtc gggataaata cggattaact
     4681 gcttgaatgt cgacgctggc caaaaaaata aacttccttt accgatggaa accaaattgg
     4741 cgatatattg ctgtgggggt gcgtcaaaaa gcggcggcag tccggcaatt tcacccgctt
     4801 caaaaatttt aatgacgacg ctatgtctat cttcgcccag catataccac ttaaaatacc
     4861 ccgattcgac aaaaaaaaca ccacggtatt tgtcgttttc tcgaaataca tgttcgccat
     4921 tcttaaaatg gatgtgcacg gcggctttgc gcaatcttcc ctcgagttcg gaaggcattg
     4981 ctttccaaaa agaaataaaa ttgttttctt tgtgctgtcc tttttttatc atacgtgtaa
     5041 cgttatataa aaatacagaa gggtgcaagt tattagttat gagcatggac gacaacctta
     5101 aaattctatg gtgattattt ttgtagacgt ggccaaacct cacaatctaa ttctttcttg
     5161 ccgcttgctg tcaaaactgt aagtgctcca cactacgcca tgacttcgat caattttgca
     5221 aaacttaatt ctcaaaaaac tgaactcctg caaaatatat cgcactacaa gcgctttgca
     5281 aaaaacgaga atgtgtttga agagggcgat gtctacaggg ggccatacat tgttgtcgaa
     5341 ggccaattca aagtttttat gctgggcgat gccggcaaag aaagcatcat gcacgttttt
     5401 cgcgaaggcg agcttctggc tggagggccg ctttttttag gagggaccta ccccgcttcc
     5461 tgcgcttcgc tcagcgacgg ttgtctcatt gcatttgagt atgatcgctt gaaaaaaatt
     5521 attgataccg atgaatccat ccataattac tttctacagc ggacactaaa attattacca
     5581 cgtttgaaac aaaaaattga aagcttggcg ttgaaaagcg cagagcagcg gatcattaat
     5641 tactttaaat cgctgggagc cgaccgtgcg ccggtagctc ttgatatacc caaaaatcaa
     5701 attgctgcac tgttggattt aaccccggaa tcagtaagtc gtgtactcaa ccagcttatc
     5761 caacgggaaa ttttgtctgt cgacgaaaaa acgtactccc taaaaaaata gccgcccaat
     5821 agcaagagcg cgcccgaagc catccagaaa atgttaagct tttgattttt tacgcactgt
     5881 gcgcgtaaaa aatcaaaatt tagaactttt ctatttacta gttttagttt acgctgtgaa
     5941 aagaatataa agaagaatga atgcttaaac aaacgctttt ttccctcgcc actgcgctta
     6001 ttttactgtc gtgctccagt cggttcgtca aaaaagaaga tgtgcaagaa ttcagtaaag
     6061 aatatgaaaa gacgtatgta ctcaaaaaaa atatcgaagt cggtaacttt gaaacactga
     6121 acgaaggcgc caaagtgcgc gtctatttta aaacggcggg cgactatatc gcggtgtacg
     6181 cctatcccta ctcgcaaaaa cgtgaagaag cggtgggtaa aaacatccta caagtttttg
     6241 acgccgattt ccccgataaa aagtttaatc cacaggttat ccgcgataag ctcgcgccgc
     6301 tcattgccga agaggatgcg gtaaaaaccg aaaaaaagtc gacaaaaact aaaaaagaaa
     6361 aaaagaaaaa taaaggaaaa aaataatatg aaaatcggta tctttggttt aggcaccgtt
     6421 ggccagggcc tcgtcgatat aatacgccaa aaaaagagcg atctccaaat tgccgccgtg
     6481 gttgatcgga gctttgctaa aaaagaacaa attttacaag ggatacccgc atcggcagac
     6541 aaagatttta ttttgaaaga cgaatccatc gataccgtgg ttgaactcat gggtggtatc
     6601 gacgattcgc tctacattgt gcgatccgca atcgaagcgg gtaaaaatgt agtgaccgca
     6661 aacaaagcgc tcttggccga acacggctat tcactcttcc acttagcgca ccgcatggcc
     6721 gacaagggct ctccccgtat tctttacgaa gctgccgtgg caggggcaat ccccattatc
     6781 cgcaacatcg acactatctt tcgcaacgaa cagattaccc gtctcatggg gattatcaac
     6841 ggcacaacga actacatcct cacccgcatg cgcaaagaaa gcctcaacta cggcgatgtg
     6901 ctcgaggccg cacaaaagca aggcctggcc gaggccgacc ctactctcga cgtgggcggt
     6961 tacgacgcgc aacaaaaact cgcgctttta ctgtcgctta tcactggtaa atgggtcgag
     7021 gcgaaaaata tccccgtgcg cggcatcacc gagttgtcga gtcaagacgt ggtgtgggcg
     7081 gaacgttttc aaaaccgcat ccgcctcgtg gccgattata cgtacgacaa cggacaacac
     7141 tacatgcatg tcacacctgc gctcgtcgac gaacgtaacg tcctttacga cattgaatac
     7201 gagaacaatg cgattttatt ccatggcgaa tactcgggcg agcatctttt tatgggcaag
     7261 ggcgccggca aatatcccac ggcgttttcg gtgtattccg atttacttac gcttaaatcg
     7321 caagcaggtt gcgctctacc gcagcgggag ttgagcacca actatttagc cattggcgac
     7381 attgccgaca ccagctcgtc gttttattta cgcttacgcg tgaaagacca acccggtgtc
     7441 ctcgcggcgg tggcacaaat tttgggcaac aacgccctct cgattgcgtc ggtacaccaa
     7501 gaccattcgg cggtggctgc gggcgaagtc gacattgtca tccacacgca ctcggccaag
     7561 cgcagcggtt tttataaagc gctccaagaa attaaaaatt tgaaaaactt tgtgctccat
     7621 caggtttatt tgccggtgct ccgctaggat aagcgcagct caaaaaccaa acagcgtgcg
     7681 cgagagcgcc tccaacttgc gcgagcggtc gtagtcttcg ttgcgtgcgg cgagcgcagc
     7741 ggtgttggcg attgctcggc gtgcgataat ctcaaaaggc actgcatcta aaaggcgatt
     7801 agaataattg ggaaaatttt cacgcatgag ttggtcgagc ttgtcgcggt cgataactgt
     7861 gcctcgatca aagacattga taaaaagttc taagttgccg tactgccatt ttaccaggaa
     7921 acgcgcgggg gtattcacgc cataaatggg aaatcccaag cgcaagccag cgattagata
     7981 tatcattgct atcgagagcg gtatcccagg tatgcgttcg caaagcaccg cttgtggcga
     8041 ataggcctcg gtgcagttga ttgcgttatc gctgccgcta atgccgtttt tttcaaaaat
     8101 tgtttcattc aatgcagaaa gaatttcgtg cataattttc aaatcgtcga agcggccttt
     8161 aacccgcctt ggggttaaat ggacgctctc gagtcgttgg cgtaaatcca cttcaagact
     8221 atctaaagcg gcacagtatt catcccacag cttaaaaggg gtggcaagaa gaccggtgag
     8281 gtatgaaata cgttcaatcg cgtacacgtc attattcgag cggggcaaca catgtccaag
     8341 ctgattatac acaaaagcag ttgtgtaatc aatttcgagt gtacgcagtt tgtggcgcaa
     8401 ggcgaaatca ggcactgcgt cgatattctg aaaaatttct gcccacgacg cttcggtggc
     8461 aatgagttgc atgaacgctt gcgaggtagc caaatttttg ttgttcgcta aatcttttcc
     8521 tatttcgatg aggagtgcga gtatgttgtg tgagcgtagc ggcataatgt taattaaaaa
     8581 ggattgtttt attctcgttt ttttcgccct gtgataattt gatccattcc tttgagcgcc
     8641 gattcgatca tgccggctgt catctctggt cccagcttcg gcgaatcttt gagcgctgca
     8701 agaaatcctt ttttcacgcg ttcttcaaaa cgcggcagaa ggcgatcccc cgcttcttcg
     8761 actttgagcg agattctgtc gggtaaatcg tcgataggtt tttcaatttc gtcgtaataa
     8821 agtttgcgcg caagaaaata gacaagcgcg gctgtcacaa tcgacgttat cgctgcggtg
     8881 ggtataataa cataccagat aatttctgga aattgcataa ttgcccgtct atggagagat
     8941 acactacgca aaccattttt tgtcggtcac tcagtctatg gattccttat cttatcttac
     9001 tcactacgcc gccgtttgat aaaacttgga ataaacgctc aacttctttg ggcttcgctt
     9061 tcgatgtatt aaggactcta ttgagggatt ttttcttcaa gagcgctttt gcgattttgt
     9121 actgattttc tgtgagaagc tctatcaaaa tttcgccatg tcttttatac atcaaaataa
     9181 attcagcagt ggcaaaatta cgggggatgt acccgtgtgg gatttttgct ctatttctcc
     9241 acagtggata aaggcggtgt gtaaaagacc acaacgatgc ggtctcaaaa aattgaaact
     9301 gtgtccgttc gggatgaatg ctttgaaaat tcaacaccga tggcacaacg ggcgcagtgt
     9361 tgaagagttg ttggaatgcg aactcagacg cggcaaggtc ccctaaaaaa gtaaattgct
     9421 tgaacttacc ccgcaagaac gcagggaatg ttgcgccgta gttattcaaa ttatacgaaa
     9481 tcgatttatg cgacgctata tattctttgc acgttttgaa aaaaagacga tcgcctaaaa
     9541 cagaccacac ggtttcgtat gtcgtgccca atgcgctcgt caaacgcgca gtatannnnn
     9601 nnnnngaaaa ataaatgaac gtcctgtatg gatataccaa ccggcatggt atactttgag
     9661 cgcggctttg tcggaaagct taccgccgcc tttgacttgg gcaagcgacg caaaattttg
     9721 attttgaatg ccctctaaaa aaactttttg tagtttgccc acatccgcgt tattcctttt
     9781 ttctgggacg cgcggcggta gaaatacttc catcacgata cgattttttc tctctttaat
     9841 gtaagcgttc tctcggattt tccccaaacg caacacttct ctttcaacaa catggaatgc
     9901 gggtattgcg tcgtcgcgtt cgacaatcat cggtattttg tgaaaccgtg cagcgtgttt
     9961 tttccacaat tccaaaacag gcgcatgcac cggctcggcg tgcgtgtcga ataaatgcgt
    10021 acccatgtcg ctgtatcctg cgatgtgaat ttctcctaca tgcgtgggat tgatacgcgt
    10081 taaaaaatca tcggcgttat aaccatgatt ttttgcgttg acgtacacgt tgttgatgtc
    10141 gagaagaatt ttacatccac ttttttgaac aatggtattg atgaaatccc actcgctcat
    10201 ttcactcgcc cgccacgtca aataactcga gacattttca agagcgatgc gtgtcttcaa
    10261 gaaattctgt acggtatcga tgtttctcac aacgatatcg accatacgca tcgtgaaagg
    10321 tagcggcaac aagtcgtgcg agttctcgcg tttaacgccg gtgaaacaca aatgatcgct
    10381 tattaaaaaa ggctcaattt cgtcggcgag ctgtttgagt ttttttaagt atgcgttgtt
    10441 gacgcctgtg gccgatccca tcgacagcga cacgccgtgc atgcacattt tgtaatcgcg
    10501 gcgcagacga cgcactacct cgcgcggata gccttcggta tcgatataat tttccgaaag
    10561 aacttcaaaa aaatcgacgt ttgccttgtg tttggcgaga tacgggaaat gttttgccct
    10621 tagcccaacg ccgagcatgg ctattcacaa aaaaacactt gcgacttggg ttgttcttta
    10681 ttgcctgctt tgtcggtaga ataaaattcc accagcgtta ttttttctgg cgtgcagata
    10741 tctgcgtgcg tgagtcgatt tttatattcg cttgtcgaga ctgatgcgag atcttttcct
    10801 aaaggtagca cacgatagaa tgttttagcc aaacccgatt cgcgctcatt aactaaaaac
    10861 aacaatgcgc tttctttacc aaaagtaatc cctcttgagg aatgcaccgg attgctcaag
    10921 attgcgcgcg tgacaggggg attgagttcg actttgaaaa caaaattttt tgtataaacc
    10981 acttcttggt tttgattttc taaattcacc accattttgt acgtcgctgg tttttcaaaa
    11041 acaatcggtt cgcggtattc gataaactca ccatcgccga gtttgtactt ggtgacaaga
    11101 ttaggttgtg tgggtaactc gatgcggttg cccaaaagat attcggtttg gccttgttgc
    11161 acaacaggta tgctcggctc tttatatggt tttgccaagt aggcatcaat cggatacgtg
    11221 gggctatacg caccggcgac gcccgttttg tggattgagc gcactcgaaa aaagcgaatt
    11281 tcaggggcaa gagattcgcg gaacacattt tctcgagt
//
LOCUS       DAOMPD010000020        11141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2511_length_11141_cov_2.974021, whole genome shotgun sequence.
ACCESSION   DAOMPD010000020 DAOMPD010000000
VERSION     DAOMPD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_11141_cov_2.974021"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLY93_01460"
     CDS             <1..398
                     /locus_tag="PLY93_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68180.1"
                     /translation="NKFLLNNGLHTAHHEQMGLHWSQLPAAHDKIKHLVDPSLNHRSL
                     GWYIIRTYFLAPFVPSFREESMRLKRMTLSTGGSVSAAFEATAPAPSKSSGKKSARNT
                     PKRTTGKAVVKKSAVAKRAKPTAKKTAKK"
     gene            757..1248
                     /locus_tag="PLY93_01465"
     CDS             757..1248
                     /locus_tag="PLY93_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRP68181.1"
                     /translation="MAQDSSFDIVSETNWQELTNAIDQAQREIANRFDFKGTQVAITL
                     EDKEMVLIADDEGKLNQLKDVVESKLIKRGIALKSVDYQKMESAFGGNVRQKATFVNG
                     IAQEHAKKINVLIRDSKLKVRSQVEGDKVRVSGKSRDDLQAVMKLLNEAELPIPLQYT
                     NYR"
     gene            1307..2521
                     /gene="argJ"
                     /locus_tag="PLY93_01470"
     CDS             1307..2521
                     /gene="argJ"
                     /locus_tag="PLY93_01470"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRP68182.1"
                     /translation="MVIFIDHCYIKIVRNLSISENFKSKPLFDIPKGYRTFITNAGIK
                     DKTLDLGVVVSDATANAAAMFTSNKVKGNPVQVGIKHIKGGKVRAVVVNSKNSNVATG
                     KPGYDDCLRICKKAAQELNTPVTQIFPSSTGVIGRRLPTDKIIDALDSLSAKLIDVDF
                     AGFAEAIMTTDTFPKYASRKIGNATLLGVAKGSGMIEPNMATMLSYFFTDAEIPAASL
                     KAILKRVVEPTYNSLSIDSDMSTSDTVMVMANGVQGKVPLAAFEKAFREIATELTRML
                     ARDGEGATKLFTVTVKNAATELDARAIGKSIINSPLVKTAIYKGDPNWGRIFMAVGKT
                     PGVKVDPQKIKIRWGGKSYSNDELPALSEYLKANEELELEVSLSAGAKSWTVYGCDLT
                     EAYVRINAYYTT"
     assembly_gap    2564..2573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2574..3744)
                     /locus_tag="PLY93_01475"
     CDS             complement(<2574..3744)
                     /locus_tag="PLY93_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP68183.1"
                     /translation="MIAFFRSLVVAGEELDAHRRRYVVNTNVYALSVIFFSLPYPFVY
                     LYLHVDIDPLTLWGSVLFILAYATVLFFNRLKVYTLAKLLFYITALVQLFIICIFFGQ
                     AAGLDQFFYAAFIGVFFVMDRHQKVLIIPALVIIVAEYILLHFIYAHFAPVIKVEPET
                     AAIIHHLTSFAVFLLLGLQCLFFYWGNLRAEENLEEARRQSDRLLLNILPEPIADELK
                     EKGMVEPVEHDAVTVVFTDFVGFTSSSEFRNPVDLLKDLDDCFSAFDAIIEKHGLEKL
                     KTIGDAYMFAGGVPSDLVDNATCCVAAATEIIAYVKKRREENSRFWQIRVGIHTGHLI
                     AGLIGRKKFIYDVWGDTVNTASRLESSGEPGRINISHATYELVKEKFRCTPRGEIP"
     gene            complement(3741..5450)
                     /locus_tag="PLY93_01480"
     CDS             complement(3741..5450)
                     /locus_tag="PLY93_01480"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRP68184.1"
                     /translation="MRNFEESLAIAEAWTKPPFDTETQNQVRSLLKLAEKEREKEIFD
                     RFGYDLAFGTAGMRAVMDVGTARINEYTIRRVSCALAHVIKKENGKSIVVGYDGRRDS
                     AKFAEVARNEFLGMGLDVFAFDRILPTPLVPFAVRKLNADCGVMITSSHNAAPYNGYK
                     VYNAQGAQILSPFDTDVMAAMQNLPYSGAATASTGKLTLLDETLLREYVLWATSEADF
                     SPKENRNLKIVFTPLHGAAGAMMNSVFYRAGFKNFLPVPQQYEPRPDFPTVKAPNPEN
                     KDSLDMAIRMAKEENADLILATDGDGDRVGIAYQKDGSYVSLTGNQIGTLYLFYLLTK
                     LKEKNINLTKCFALSSVVSTPLTKKICDAFGVRYFATLTGFKNMGNKADAYIRANAGV
                     RMVLAFEEAFGVTIGDSRDKDGLVSCLLGAHIAADGGIEAWLKKMYSAHGYAAEDAVE
                     KEFLGAEGISAMNAVVDALRQKPITEFLGALVTSVRDFKTGVEKRAGKEIPIEDIPTQ
                     NLLYYTNSAGDWFALRPSGTEPKLKAYVGVREDEFSASSESRLRERLTALCHFAQALL
                     ESP"
     gene            5496..6257
                     /gene="tpiA"
                     /locus_tag="PLY93_01485"
     CDS             5496..6257
                     /gene="tpiA"
                     /locus_tag="PLY93_01485"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRP68185.1"
                     /translation="MANTRIPLIVGNWKMNLLLGEATALANTIKDAAAKYTDRDVLLY
                     PTFLQLTHVAEIIKGSRVLLGAQNCSAEKEGAFTGEIAAAQIKDAGAGYILIGHSERR
                     HILKENDAFLKTKIDRTLEQGLMAMLCVGETLDERESGRAETVVLNQLNAALSHLTKD
                     AWKHIAIAYEPVWAIGTGKNATPEDAQAMHAAIRKRIAALNADAAPVTRILYGGSVKP
                     DNVATLMAKEDIDGALVGGASLKADSFSELIKLAK"
     gene            6266..6724
                     /locus_tag="PLY93_01490"
     CDS             6266..6724
                     /locus_tag="PLY93_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68186.1"
                     /translation="MDDFIKLSPLLSAMLVSLGFILALIQYKRQLAYKKLENLQKIWK
                     DFLNDDHLFTFFSALDQAPDKRAMKRVADTPYQTKLRFIGLLDEVALLVFDGDVKKEH
                     AIYLFQWLYYYAFKNETTMLHLWKNLGGATEAQKPYWEKARNLAVECNPE"
     gene            complement(6727..8679)
                     /locus_tag="PLY93_01495"
     CDS             complement(6727..8679)
                     /locus_tag="PLY93_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP68187.1"
                     /translation="MIEISNLSKVYTMGGESVYALNDVSLAIAPDEFVAIMGRSGSGK
                     STLLQILGLLDTPTSGSYRLFGKEVAHLTEDELAIYRRETLGFIFQQFHLLPRLNALE
                     NVAMPLLYSGLTNLPESPERLLTDVSLGDRMHHRPSEMSGGQQQRVAIARSMVNSPRI
                     LLADEPTGNLDIASQTDILHLLSEMHNSGIGVVVVTHDDEVGEATNRIIRMQDGRIIS
                     DKSRKKIITRPEFIHVQKRNLEKHNPFTYFLQLLEEGYRAVLANKLRSALSMLGIMIG
                     VASVIAMLALGRGAQNSIESQLKGLGANVMVVRPGAARVGGVAQETGSPARISYDDIG
                     YIDSSVTGIKGVSGNVTSRAQISYANKNWNTQVQGSSVSYGEIRNAIPFVGRMFSEHE
                     NKTRARVAIIGLTVVRELYGEENPIGTFIKINKINFQVIGIMPAKGGTGWRDQDDIVV
                     VPLQTAMYRLMGKIYVDSLDIEGDGRTSLDDVGDAVKRALHSRHRVPLSMQDDAYQVM
                     NLADIRAAVSSTSSTMSVLLAAIAAVSLLVGGIGIMNIMLVSVTERTKEIGLRKAVGA
                     RRKDILYQFLIETLAVSISGGLLGILLGVAISLSLSSFAGWATSISSMSVFLAVFFSV
                     FTGIVFGIYPARKAARLNPIEALRHE"
     gene            complement(8688..9656)
                     /locus_tag="PLY93_01500"
     CDS             complement(8688..9656)
                     /locus_tag="PLY93_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRP68188.1"
                     /translation="MKKIFFIFTVLLIAAMTIYFSFGRKKKNEITYKEVKPVRTDIRI
                     TVTSTGTVSPQGRIEMKPPVPGRIDSVSVKEGDKVRKGQILAWMSSTERAALLDSARA
                     VSTEEYKRFQEYYKATPIFAPVSGQIILRSVEPGQTVAASDVLLVISDKLQVVAKVDE
                     TDIARVKPGVTAEIILDAYSKNVIKAKVSAIAFEAKTVNNVTTYDVEALPNKQPDFMR
                     SGMTANVTFILSERVGALALPMAAIQFNKESPFVLVKDAKGKPVQQAIETGESDGGLT
                     EIVAGLNDDAVVLIPGIQTKSDDEGSNPFMMKRGGNKKSGGKGAPH"
     gene            complement(9659..10912)
                     /locus_tag="PLY93_01505"
     CDS             complement(9659..10912)
                     /locus_tag="PLY93_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP68189.1"
                     /translation="MTRILLVSLLPAFLWAEHITLDFQTAVKNLSTQNRDILAARQNR
                     DTAQYNYRATYAAFLPQVNLGLNYTQGNSATTAQLANQSNRYELYSASMGVSQNVFSG
                     LADYYKIKAAKANMDLSEANFRQTAAKALYDLKAAWAQLALSYKQNELALSTLKRRKD
                     NRKMIELRFKGGSENKGSLLMAEAYETQANRDVNQSLRQRINAETELKRVLYLPQDAT
                     IAISGLFQRKNPPEEVLLVDTAKKTPEYLQALAKELNAEAGADQSMSVFFPTVTASGS
                     LFRQGPDFFPQGDRWSVTVGASYPIFTGGKDYYTVKAAKSAFEEAKQNTRSVEATRIS
                     RLQQTLQSYRAAEESAKISTSLLEASNVRAQIARVKYMSGLLSFDQWDIIENDLISRQ
                     KDALQTRYDLDVAQATWEQTLGEWD"
BASE COUNT         3002 a         2774 c         2575 g         2780 t
ORIGIN      
        1 acaacaaatt cttactcaac aatggactac acaccgcgca ccacgaacaa atgggcttgc
       61 actggagcca actgcccgcc gcgcacgaca aaattaaaca cctcgtcgat ccttcgctca
      121 accatcgctc tctcggttgg tatatcattc ggacgtattt cttggcgccg tttgttccct
      181 cattccgtga agaatcaatg cgcttgaaac ggatgactct ctccaccggc ggcagcgttt
      241 ctgctgcgtt tgaagccacc gcacccgctc cgtcaaaatc ttctggaaag aaaagcgcgc
      301 gcaacacccc caagcgtact acgggaaaag cagtggtgaa aaaatctgct gtagcaaagc
      361 gtgcaaagcc aacggcgaag aagactgcca agaagtagca gcgatagact tcaataacgc
      421 cgtagcgaaa acattctcaa cgctgaaaga aaaataagcc cgccgggtat tacgtaccta
      481 atcacactct ggggcaattt gtgaagctca aacccaaaag tgagactcag atattttcaa
      541 ccaaggtagc gcctattaac ggacaatttt cccttctttt ttgacccttc catcactcgt
      601 ggggatagtt accccctaat gtgacataat atgctaaaaa ctaccgtttc cacaccagtg
      661 ggttcaaaaa actccagaat ggatacatta agagcctgag attagagttg ccgcccactc
      721 aacgagttag gctatcaaca ctgcacggag aaaataatgg cacaagattc atcgtttgat
      781 attgtttcag aaaccaactg gcaggaactc accaatgcta tcgaccaagc gcagcgtgaa
      841 atcgccaacc gattcgattt taagggcact caagtggcaa tcactttaga ggataaagaa
      901 atggtgctta tcgccgacga cgaaggcaaa ctcaaccaac ttaaagatgt ggttgaatcg
      961 aagctcatca aacgcggcat tgcattgaag tcagtcgatt accaaaaaat ggaatcggcg
     1021 ttcggtggca acgtgcgcca aaaagcaaca tttgtcaatg gaattgcaca ggaacacgcc
     1081 aagaaaatca acgtcctcat ccgcgactcc aaactcaaag tgcgctcaca ggtcgaaggc
     1141 gacaaggtac gcgtgtccgg caagtcgaga gacgatttac aagcggtgat gaagctctta
     1201 aacgaggcag agctaccgat accgttacaa tacacaaact atcgataagc tttaaacact
     1261 acgttttaaa tttactaatc ggtatatcca tatttacaat acaataatgg tcatttttat
     1321 tgaccattgt tatattaaaa tcgtgagaaa tctctctatt agcgaaaatt ttaagtcaaa
     1381 gcccctcttt gacatcccta aaggctatcg cacttttatc acgaacgctg gcatcaaaga
     1441 caagacactc gatttaggcg tggtggtttc cgatgccact gcaaatgcag cggcgatgtt
     1501 cacctcaaat aaagtgaaag gtaatcccgt gcaagtgggc atcaagcaca tcaaaggcgg
     1561 caaagtgcgc gcggtggtgg tgaactctaa aaactccaac gtggcaacag gcaagccagg
     1621 ttacgatgat tgtctgcgta tttgcaaaaa agcagcgcag gagttgaata cccccgttac
     1681 acaaattttc ccttcatcta ccggtgtcat tggccgccga ttacctacgg ataaaatcat
     1741 cgacgcgttg gattcgctct ctgcaaaact gattgatgtc gatttcgccg gttttgccga
     1801 agcaatcatg accaccgaca cattcccgaa atacgcctcg cgtaaaattg ggaacgcaac
     1861 cctcctcggt gtagccaaag gttcgggaat gattgagccc aacatggcga cgatgctttc
     1921 gtattttttc accgatgcag aaatacccgc ggcgagtctt aaagcaatcc tcaaacgcgt
     1981 cgtcgagcct acgtacaatt cgctctctat cgatagcgat atgtccacct cggacacggt
     2041 gatggtgatg gccaatggcg ttcaaggtaa agtaccgctc gctgcgtttg aaaaagcgtt
     2101 ccgcgaaatt gccacagagc ttacgcgcat gctcgcgcgc gacggcgagg gagcgacgaa
     2161 actttttacg gtgacggtga agaacgccgc caccgaacta gacgcgagag cgattggaaa
     2221 aagtatcatc aattcgccac ttgtaaaaac agccatctat aaaggcgatc ccaactgggg
     2281 gcgtatcttc atggcagtgg gaaaaactcc aggagtgaaa gtcgatccac aaaaaattaa
     2341 aattcggtgg ggaggaaaat cctactcaaa cgacgaatta cctgcgctgt ccgagtacct
     2401 caaagcgaac gaagagttag agttggaagt atcgctcagc gcaggtgcga aatcgtggac
     2461 agtttatggc tgtgacctaa ccgaagcgta cgtgcggatt aacgcgtact acaccacata
     2521 agagttgaga ccatccacaa aatacatcgc gagcggcccc ttgnnnnnnn nnncggggat
     2581 ttcaccgcgt ggggtgcagc gaaatttttc tttcactaac tcgtatgtgg cgtgagagat
     2641 gttaatccgc cctggctctc ccgacgattc caaacggctg gcagtgttta ccgtgtcgcc
     2701 ccacacatcg tagataaatt ttttgcgacc aatgaggcct gcgataagat gtcccgtgtg
     2761 gatgccgacg cggatctgcc aaaaccgact gttttcttcg cggcgttttt taacgtaagc
     2821 gataatttct gtggcggctg ccacgcaaca cgtcgcgtta tcgaccaagt cggagggaac
     2881 gccgccggcg aacatatacg cgtcgccgat ggtcttgagt ttttctaaac cgtgtttttc
     2941 aataatcgcg tcaaacgcag aaaaacaatc gtcgagatct tttaacaaat ccacgggatt
     3001 acgaaactcg gaactcgagg tgaatcccac aaagtcggtg aagaccaccg tcaccgcatc
     3061 gtgctccacc ggttcgacca tacccttttc tttgagttcg tcagcaatcg gttcgggtaa
     3121 aatgttgagg agcagtcggt cggattgtct tcgtgcctct tcgagatttt cttcggcgcg
     3181 taaattcccc caatagaaaa aaagacattg caaccctaaa agtaaaaata ccgcaaacga
     3241 tgtcaagtgg tggatgatgg cggccgtttc aggttcaact ttaatgacgg gggcaaagtg
     3301 tgcataaata aaatgcagca gtatatattc ggcgacgata atcacaagcg cagggataat
     3361 aagaaccttt tgatggcgat ccatcacaaa aaaaacgccg ataaacgccg cataaaaaaa
     3421 ttgatcgagg ccagcggctt gtccaaaaaa aatacagatg ataaaaagtt gaaccaacgc
     3481 ggtgatataa aacaaaagtt tggcgagagt gtacactttg aggcggttga aaaagagtac
     3541 tgtcgcgtac gccagaatga aaagtacaga accccaaagc gtaagcggat cgatatcgac
     3601 gtgtaaatac aaataaacaa aggggtaagg taaactaaaa aaaattacgc tcaaggcata
     3661 aacgttcgtg ttgacgacat aacggcggcg gtgcgcgtcg agttcttccc ctgccacgac
     3721 gagcgatctg aaaaacgcaa tcatggggac tccaaaagag cttgcgcaaa gtggcagagc
     3781 gcggtgaggc gttcgcgtaa acggctctcg ctcgatgcgg aaaattcatc ttcgcgtacg
     3841 ccgacgtatg ctttgagttt aggctcggtg cccgaagggc gcagcgcaaa ccaatcaccg
     3901 gcagagtttg tataataaag aaggttttgg gttgggatgt cctcaatcgg aatttctttt
     3961 cctgcgcgtt tttccacgcc agttttgaaa tcacgtaccg acgtaactag cgcgcctaaa
     4021 aattctgtaa ttggtttttg ccgcagtgca tcgacaactg cgttcatcgc agaaatccct
     4081 tcggcgccta aaaattcttt ttcaactgca tcttcggcgg cgtacccatg cgcgctatac
     4141 atttttttga gccacgcttc aatgccgcca tcggcagcga tgtgcgcgcc caaaaggcag
     4201 ctcacgagtc cgtctttatc gcgcgagtcg ccaatggtga caccaaacgc ctcctcaaac
     4261 gcgagcacca tgcgcacacc agcgttagcg cggatatacg catccgcttt attccccatg
     4321 ttcttaaaac cggtgagcgt cgcaaaatag cgcactccaa acgcatcgca tatttttttt
     4381 gtaagcggcg tggagaccac actcgacagc gcgaagcatt ttgtaagatt gatatttttt
     4441 tctttgagct ttgttaataa ataaaaaaga tagagcgtac caatttggtt gccagtgagc
     4501 gaaacgtagg agccgtcttt ctgatacgca atccccacgc ggtcgccgtc gccgtcggtg
     4561 gcaaggatta aatccgcgtt ttcttctttc gccatgcgga tcgccatatc gaggctgtct
     4621 ttattctcgg ggttgggggc ttttaccgtg ggaaagtcgg ggcgcggttc gtattgctgt
     4681 ggcacgggga gaaagttttt gaaaccggcg cgataaaaaa ccgaattcat catcgcgccc
     4741 gcagcgccat gcaacggggt gaatacaatt ttcaaatttc gattttcttt gggggagaaa
     4801 tccgcctcgc tcgtcgccca caaaacatac tcgcgtaaaa gtgtttcatc caacaacgtg
     4861 agcttccctg tgcttgcagt agctgctcca ctgtacggta agttttgcat cgccgccatc
     4921 acatcggtgt cgaacggcga taaaatttgt gcgccttgcg cgttatacac tttgtaaccg
     4981 ttatacggtg cggcgttgtg gctcgatgtt atcatcacgc cgcaatcggc gtttaatttt
     5041 ctgacggcaa aaggaactaa cggtgtcggc aaaatgcgat cgaaggcaaa aacatcgagc
     5101 cccatgccta aaaactcgtt gcgtgccacc tcggcaaact tagctgagtc tctacggcca
     5161 tcgtaaccga cgacaatact tttaccgttt tcttttttta taacgtgtgc aagcgcacaa
     5221 ctaacgcggc ggatagtata ttcgttgata cgcgccgtac caacatccat cacagcgcgc
     5281 atgccagcgg taccaaacgc taaatcgtaa ccaaagcggt cgaagatttc tttttcacgc
     5341 tctttttcgg caagcttcag gagagatcgc acttggtttt gtgtttcggt gtcgaacggt
     5401 ggttttgtcc atgcctctgc gatggcgagt gattcttcaa aattgcgcat gtaatttttt
     5461 cgtttacagg catacgccgt aaaacactta actccatggc aaacacacgc attcccctca
     5521 ttgtaggtaa ctggaaaatg aatcttttac tcggcgaagc aacggccttg gcgaacacca
     5581 tcaaagacgc tgcggcaaaa tacaccgaca gggacgtgct actctacccc acgttcttgc
     5641 agctcaccca cgtcgctgaa atcatcaaag gctcgcgcgt tctactcggc gctcaaaatt
     5701 gctccgccga aaaagaaggc gcgttcaccg gtgaaatcgc cgctgcacaa attaaagacg
     5761 ccggtgcagg gtacatcctc attgggcatt ccgaacgtcg tcacatcctc aaagaaaacg
     5821 atgcattcct caaaacaaaa atcgaccgca ctttagaaca ggggctcatg gcgatgctct
     5881 gcgtgggtga aactttggat gaacgcgaaa gtggccgcgc agaaaccgtc gtgcttaatc
     5941 aactcaacgc cgcgctttca caccttacaa aagacgcgtg gaaacacatt gcgattgcgt
     6001 acgaacccgt atgggcgatc ggtaccggta aaaacgccac ccccgaagat gcgcaagcaa
     6061 tgcacgcggc catccgcaaa cgcatcgccg cgctcaacgc cgacgctgcg cctgtaacgc
     6121 gcattcttta cggcggctcg gtgaagcccg acaacgttgc aacactcatg gcaaaagaag
     6181 acatcgacgg cgccctcgtc ggtggggcga gtttgaaagc agatagtttt agcgaactaa
     6241 ttaaattggc gaagtgagtt taagaatgga tgattttatt aaactctcac cactcttgag
     6301 tgcgatgctt gtctctttgg ggtttatact tgcgttaata caatataagc ggcagttggc
     6361 gtacaagaaa cttgaaaatt tgcaaaaaat ttggaaagat tttcttaacg acgaccacct
     6421 ttttacattt ttttccgcgt tagaccaagc acccgataaa cgtgcaatga aacgtgttgc
     6481 agatactcct taccaaacaa aattacgttt tatcggcttg ttagacgaag tggcattatt
     6541 ggtatttgat ggcgatgtta aaaaagaaca cgcaatctat ctcttccaat ggctttatta
     6601 ctatgctttc aaaaatgaaa caacgatgct tcatctctgg aaaaatttag gaggtgctac
     6661 tgaagcacaa aaaccctatt gggaaaaagc acgaaatctg gcagtagaat gtaatccgga
     6721 ataacgtcac tcgtgtcgca acgcctcaat ggggttgagt cgtgccgctt tacgtgcagg
     6781 ataaatccca aacacaatgc cagtgaatac cgagaagaag accgccaaaa ataccgacat
     6841 tgaactgatg gatgttgccc aacctgcaaa agaagaaagt gagagcgata ttgcaactcc
     6901 aagaaggata cctaagaggc caccggaaat actcaccgct aaagtttcga ttaagaattg
     6961 ataaagaata tctttgcgcc gcgcacccac cgctttacgc agaccaattt ctttagtacg
     7021 ctcagtgacc gaaacgagca tgatgttcat aataccaata ccgccgacca agagcgacac
     7081 tgcagcaatc gccgccaaaa ggacagacat cgtcgaggaa gtcgatgaca cagcagcgcg
     7141 gatgtcggct aaattcatga cctgataagc gtcgtcttgc atcgagagcg ggacacgatg
     7201 gcgtgaatgg agtgcgcgct tcacggcatc acccacatcg tcgagactgg ttctaccatc
     7261 gccttcaata tctaatgagt cgacataaat ctttcccatg aggcggtaca tcgccgtttg
     7321 taacgggacc acaacaatat cgtcttgatc gcgccaaccc gtgccgcctt tagctggcat
     7381 gataccaatc acctgaaaat taattttgtt gattttaata aacgtaccga tgggattttc
     7441 ttcaccatag agttcacgca caacggtgag gccgataatc gcgacacgcg cccgcgtttt
     7501 attttcgtgt tcgctgaaca tacgcccgac aaaaggaatt gcgttacgga tttctccata
     7561 tgacacactg gaaccttgta cttgggtatt ccagtttttg ttagcgtacg aaatctgtgc
     7621 acgacttgtc acgtttcccg aaacgccttt gatgcctgta acgcttgaat caatataccc
     7681 gatgtcgtcg taactaatac gtgcagggct tcccgtttct tgcgctaccc cgccgacgcg
     7741 cgccgcccct ggacgcacca ccatcacatt tgcgcccaag cctttgagct gcgactcgat
     7801 ggaattttgc gcgccacgcc cgagagcgag catcgcgatt accgacgcaa caccaatcat
     7861 gatgcccagc attgaaagcg cgctacgcag tttattggcg agcaccgcgc ggtagccttc
     7921 ttctagaagt tgcaaaaaat aagtaaacgg gttatgcttc tcaagattcc ttttttgcac
     7981 atgaataaat tcagggcgag ttataatttt ctttctactt ttgtcgctaa taattcttcc
     8041 gtcttgcata cggatgatgc ggtttgtcgc ctcacccact tcatcgtcgt gcgtcaccac
     8101 aaccaccccg atgcccgagt tatgcatctc actcaagagg tgtaagatgt ccgtttgcga
     8161 cgcgatgtct aaattgccag tgggttcgtc ggcgagtaaa atgcgcgggc tattcaccat
     8221 cgagcgtgca atcgccacac gctgttgctg cccccctgac atttccgaag gacggtggtg
     8281 catccggtcg cccaagctga catcggtcag caggcgttcc ggcgattcgg gtaagtttgt
     8341 taagcccgaa taaagaagcg gcatcgcaac attttcaaga gcattcaagc gcggcaacag
     8401 atgaaattgt tgaaaaataa atcccaacgt ttcacgccga taaatagcca attcgtcttc
     8461 ggtgagatgc gcaacctctt ttccaaaaag gcgataactc cccgacgtgg gtgtatccaa
     8521 caaccctaaa atttgtaaga gcgtcgattt tcccgaaccc gagcgcccca tgattgcgac
     8581 aaattcatcg ggagcgatgg cgagcgaaac atcgttgagt gcatagacgg attcaccacc
     8641 catcgtataa actttactga gatttgaaat ttcaatcatg cctgatttta atgtggcgcg
     8701 cctttaccgc ccgacttctt gttgccgccg cgcttcatca tgaatgggtt gctgccttcg
     8761 tcgtcgcttt ttgtttgaat gccggggatt aaaaccactg catcgtcgtt gagtcctgca
     8821 actatttccg tcaacccacc gtcggattca ccggtctcga tggcttgctg tacgggcttt
     8881 ccttttgcgt ctttaacgag tacaaacggc gattctttat tgaattgaat cgctgccatc
     8941 ggtaacgcaa gcgctcccac gcgctcgctg agaataaacg ttacgttggc cgtcataccg
     9001 ctgcgcataa aatcgggttg tttattgggg agcgcttcaa cgtcgtacgt tgtaacgttg
     9061 ttgactgtct tcgcttcaaa tgcaatggcg cttacttttg ctttaatcac gtttttggaa
     9121 tacgcatcga gaataatttc agccgtcaca cctggcttta cacgggcgat gtctgtctcg
     9181 tcgacctttg caaccacttg tagtttatct gaaatcacca aaagtacatc ggatgcggca
     9241 acggtttgtc cgggttccac actacgtaaa ataatttgcc ctgaaaccgg cgcaaagatg
     9301 ggcgtcgctt tataatactc ttgaaaacgt ttatactcct cagtactcac cgcgcgtgca
     9361 gaatcgagga gcgcggcgcg ctcggtggag ctcatccatg ccaggatttg ccccttgcgg
     9421 actttatcgc cttctttgac agaaacacta tcgatgcgtc ccggcacggg gggtttcatt
     9481 tcaatgcgcc cctgcggtga aacagttccc gtgctggtga cggtgatgcg gatgtccgtg
     9541 cgtaccggtt ttacttcctt gtaagtgatt tcattttttt tctttcgccc aaaagaaaag
     9601 taaatcgtca tcgccgctat gagcaagaca gtaaaaataa aaaatatttt tttcataatc
     9661 aatcccattc accaagggtt tgttcccacg ttgcttgcgc aacatctaaa tcgtagcggg
     9721 tttgtaaggc atctttttgt cgggagatta aatcgttttc gatgatatcc cactgatcga
     9781 acgagagaag cccactcatg tatttaacgc gagcgatttg tgcgcgcaca ttactcgctt
     9841 ctaaaagcga cgtactaatt tttgcgctct cttccgccgc acggtacgat tgtagcgttt
     9901 gttgcaatcg ggagatacgt gttgcttcga cactacgtgt gttctgtttt gcttcctcaa
     9961 acgcgctttt agccgccttc acagtataat aatccttccc tccggtaaaa ataggatacg
    10021 aagctcccac tgtcaccgac caacgatcac cttgcggaaa gaaatccggc ccttgacgaa
    10081 agagcgagcc ggacgccgtt accgttggaa aaaataccga catcgattga tccgcacccg
    10141 cctcggcgtt aagttccttc gcgagtgctt gtagatactc aggtgttttt tttgcagtat
    10201 cgacaagcaa tacttcttcg ggaggatttt ttcgttgaaa taatcctgaa atggcgattg
    10261 tggcatcctg tggtaaataa agcacacgct ttaactccgt ttcagcgttg atgcgttgtc
    10321 tcagcgattg gttgacatcg cggtttgcct gtgtttcgta cgcttccgcc attaagagcg
    10381 aacctttatt ctcagaacca cccttgaaac gtaactcaat cattttacga ttgtcttttc
    10441 tacgtttcaa agtggatagt gcaagttcgt tttgcttgta agaaagcgca agctgcgccc
    10501 acgctgcttt caaatcgtaa agtgctttag cggcggtttg ccgaaaatta gcctcggata
    10561 aatccatatt cgctttagca gcttttattt tgtaataatc cgcaagccca gaaaaaacat
    10621 tttgactcac ccccatcgat gcagagtata actcataccg gttgctttga ttcgcaagct
    10681 gggctgtggt tgccgagttt ccttgagtgt agttcaatcc caaattcact tggggtagga
    10741 atgccgcata cgtggcgcgg taattatact gcgcggtatc tcggttttga cgcgcggcca
    10801 aaatatcgcg gttttgagtc gataagtttt taacggcagt ttgaaaatcg agagtgatgt
    10861 gttcagccca aaggaaggcc ggtaaaaggg aaacgagaag tattcgtgtc attagtggat
    10921 gaccccgttg agggtaaaag agttcccagt ttaacgcctg tacgtacccg cgaagataga
    10981 taaagcagtc gcaccactcc acagtgcgac ataattctta ttaactaaca acacttgcta
    11041 attttttttg acatttttcc catttctgca cttttttttc aaaaagtgag aaaaattaag
    11101 cgttttctgg tatttataat atagatgaga ttctttgata t
//
LOCUS       DAOMPD010000021        10652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2667_length_10652_cov_2.719260, whole genome shotgun sequence.
ACCESSION   DAOMPD010000021 DAOMPD010000000
VERSION     DAOMPD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_10652_cov_2.719260"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..894)
                     /locus_tag="PLY93_01510"
     CDS             complement(70..894)
                     /locus_tag="PLY93_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68190.1"
                     /translation="MPRVKLLVLLFTFVSPVYETGERALVKRVVAIAIDGVREVDARP
                     TIEVLRQKMEELGLHTFFRGKQQGCIPSQGTNFSLPAYATLFSGKVQADLRDNYFNGT
                     LIYKSLFDEYPDSQLFSAWPSIQKAVSNDVYTLGRVFIAGVGHKPYEDEYVMASFRLL
                     HNFENRLTFIHLGDADEFAHLGRWREYNAAIESEANYIHEIVQTSEIHAMKETVYFVF
                     TDHGRGEKVWQRHGPSLPESHTMWVLEISPFEKSTLGASCNHIAIHDAIRMDVGKP"
     gene            complement(940..2892)
                     /locus_tag="PLY93_01515"
     CDS             complement(940..2892)
                     /locus_tag="PLY93_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRP68191.1"
                     /translation="MIRKITIAFEAYRSNRFLHIFNFIFILTALVYRTALALGVRASE
                     AAPFGVEKTFSNAVALFLILGNDCIVLIVFNLIWLLLHFAWRFKNEAIVVLKRKHGFF
                     FLLLFAVFVFWNTVITASQYSSVFTMNSGFTYALFMELFSILGLHNFVTLLPVTDFLL
                     LLTPVLIYFALSLRKHWRVSRKYIATVLIAAAAVLILRPLFTMQYIPDELRLNPHEYF
                     MRDILRTLLRKKDTNKRDALSTANVYLDDPLFAKNNIDNGEGLYRSREYPNFVFIVLE
                     STGSEYIFDTEKYAEGKMPMPYLYSLTKKSLYMSQHFASNNSSPRSIFSIFSGLYESP
                     QTAFFSMKENLKVPHLINFLGSRYDAFLVTPADINWYFPKAWFKNRGFTRIYDYNTMK
                     NVPEYKAGPTPVRDEFKSVDYFLSLIRESQKPFLGIYYTFVGHWPYPDLTEEHKIVKS
                     NSSRHRYINNLFAQDQVIEKIIATLKETDKFNNTIFIIVGDHGEAFYQHPGNRVHSGE
                     SYNENIASPLVMYSPRYMISYKVKYPTTHADIVPTLLEVSGIPYKKEQFQGESLNTKR
                     VRRYVFTYGNENTLTAVSNDFIKMQILRKDEGGCRSFDLKNDWAETKNLGCNINAPQY
                     KAIEAFFRKQPDILKGYNDLCNKSGC"
     gene            complement(2889..4418)
                     /locus_tag="PLY93_01520"
     CDS             complement(2889..4418)
                     /locus_tag="PLY93_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRP68192.1"
                     /translation="MLLLILPLWPLQILRGRGFREPFLVYICLLWFLSFFTGGLNFAT
                     SIQLDETIYRFRFSSGIYTLIFAALLLVYIAEGINEARKVIIISIGCQIFLVVFQVFL
                     YQAKTAFLSPTDWTLVDQVLEPRVFNIGVSIFNTIVDLFLAVILFQFLVNRFSKIPLG
                     IIIFVALWITMMLDSAIYLGLTRPHDFMSHLTGHIIFKTSLLFLSVFPLAFFIRRFRT
                     NVNLNRGTLDIFKKLENLEKDLEKAHQELKEYAQNLEEMVEERTKEIRAKAEIPRREL
                     ELATEVQQAMLPAKNSLKTLQIHSFYKPCHEVSGDLYDFAELPDGRVFIFIADISGHG
                     VPSALVGAMCKMSLGSQDFTRANAGEILNGLSQNMQKVTTSHYLTGIILLIDPKNRII
                     EFANGGHVPCLLQSTTSSFWSIEATGTIIGSSIIAPYEFKKIQYPPDTRLILFTDGIT
                     EQKNNDREEYGNERFEKVLTRLRKKNGAQVIDEVYREVVEFSGTDKFSDDVTILIADL
                     P"
     gene            complement(4710..5057)
                     /locus_tag="PLY93_01525"
     CDS             complement(4710..5057)
                     /locus_tag="PLY93_01525"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRP68193.1"
                     /translation="MCGVPVLSESDQIATKLLANSDRWNDSGVFSRDIIDLAMIVPSK
                     KSFRLASEKAESAYGEAITVDLRKAIDHVLKTEGWLDRCIATMDIIVPQALLWKNTSR
                     LEKMYLLKLPLNK"
     gene            5474..7192
                     /locus_tag="PLY93_01530"
     CDS             5474..7192
                     /locus_tag="PLY93_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRP68194.1"
                     /translation="MRHVRAKSIFPGRVRGWMVEFKRERFIAETQIAQNPQLEYKNFR
                     SAMRAAQLQLVAEKKIMLDRRREAPHEMAALDDAIEVLESHILTLEDPYFLGEIKKRI
                     FTTGATAAAAVGTVIGAISRQFMFDSSSGIVNGQNRHFIQDMNDMKRRILYFLGIQQE
                     FTLPQNVSIVAAAKLTVSEVLALKRAGVKGIVIGETSETAHEMIMLRAMRIPCVSVSN
                     FRFFRLRKQVPAIIDADIGYIVLRPHKNLRFTESPKEETPLWSTTVKTKSGEKVQLSG
                     TLHFTSELNTLDDAPLRAQKIGIYRTEYQISEAGELPSTRQLIRHYEYLLTHYPKTEI
                     TFRLFDFSDDKNFLKSPRIEKKPDLRGIRFLLVEKKILESQLHALIKAAESTQRSIRI
                     LVPYLSETHELNSLLHIINGFGLPKNQPTIHLGAMLETPASIFTAPRWLKDIDFFYVG
                     TSDLLSALSLYNRSEAEAIQRALFSDASAALFNQLKKLSRKKPLTLCGEIVGEPWAVA
                     YLRHFEFNNFVVPVHRIATIVNILQALTKETCAHFVRQITKLDDETKRLEKARALIRD
                     ILYGQS"
     gene            7212..8153
                     /gene="pip"
                     /locus_tag="PLY93_01535"
     CDS             7212..8153
                     /gene="pip"
                     /locus_tag="PLY93_01535"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRP68195.1"
                     /translation="MRELYPEIQKVKTYTVKVSPLHTLYVEECGNPNGIPALFLHGGP
                     GAGLSTNHRRFFNPEKYRIILFDQRGSGKSTPYAELRENTLWDLICDIEVIRKKLNVA
                     KWLVFGGSWGSTLALTYAIQHPESILHLIVRGIFLCRQEEISWFYQYGAHQIFPDAWQ
                     NYVRPIPHEERHDMLGAFYRRLTSSDKKIQLEAARAWSLWEGSTICLRPDLNTSYDFN
                     EIAVSLARIECHYFMHNCFYPEDNYILNNAHKILHLSADIIHGRYDVVCPVKNAFDLK
                     EKLPKAKLTVVADAGHAAFEPGIRTALLDATDAFLLN"
     gene            complement(8150..9415)
                     /locus_tag="PLY93_01540"
     CDS             complement(8150..9415)
                     /locus_tag="PLY93_01540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRP68196.1"
                     /translation="MKKSRKINSRTVCSVFALLFIFYCRGEDKLQKAFIKVDATLNRY
                     VAENKVVGAVAVVWRDGKPFYERAVGFSDREVKVPMQMDSIFRIASMTKAITSVATLM
                     LVEEHKLKLSDKVSEYIPEFKNMQVLVKDPVTQAVSFEPAKEEITIHQLLTHTSGLSY
                     AWEKELGDLYRDAGLGRKLPMNWNLSVLKETICETAAKIGRLPLAAQPGGGFRYGYST
                     DILGCVIEKVSKKSLDKFFAERIFNPLEMHDTYFYLPKSKISRLTALYGSNKEGTAER
                     SKSKKTYPYFEGYRTNFSGGAGLVSTALDYAHFLEMIRQGGQWKDKTLLKKSTVKLMT
                     YSPYGEKASMPSTFTYGFDVTLYPNAKGQSAVQSYGWTGAYGTFYRIDPRQKMVIILM
                     TQLLPNNTDIRDTFWQALYDALAEARARY"
     gene            9525..>10652
                     /locus_tag="PLY93_01545"
     CDS             9525..>10652
                     /locus_tag="PLY93_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRP68197.1"
                     /translation="MDALFILSLVIYIIDILVLFYYGIHCFVMVRLFFKYRHNCETDS
                     SRLEDLTKKMKAWPRVTVQLPMFNEYYVAERLIDTTMLLNYPKNKLEVQVLDDSTDET
                     TELVRKKVMYYKKKGYDIKLIHRVNRQGHKAGALREAQEKAKGEFIAIFDADFMPAPD
                     FLEKTVPYFYEADDIGMVQTRWGHINADYSILTKGQSLGIDGHFTIEQIARGGSGLWM
                     NFNGTAGIWRKTCIYDAGNWSSDTLTEDFDLSYRAELKGWRFKYVVDVVNPAELPATV
                     SAYKSQQFRWCKGSIQTTVKLAPTIWRTNLPWKVKLEALTHLTNYLVHPLMIINILAT
                     LPVLYFSERLGNLPDAIIGVAAVFFSLGTFGPIAMYLVSQKV"
BASE COUNT         3000 a         2538 c         2311 g         2803 t
ORIGIN      
        1 cttcaaaaac tatggtttgg ccaaagattg tccgacctcc ccgccgaggg taagagagcc
       61 tgtgcttttt tatggcttac cgacatccat acggatggcg tcgtgtattg caatatgatt
      121 acacgatgcg cccagagtgg atttctcgaa aggactaatt tcaagaaccc acatggtgtg
      181 actctccggc aaactgggcc catggcgctg ccacactttc tcgccgcgcc cgtggtcggt
      241 gaaaacaaag tagacagttt ctttcattgc gtgaatttct gaagtttgca caatctcatg
      301 gatgtaattg gcttcgcttt caatcgcagc gttatattct cgccaacgac ctaaatgtgc
      361 aaattcatca gcgtcgccta aatgtataaa cgtcagtcga ttttcaaaat tgtgcaataa
      421 gcggaagctg gccatgacgt attcgtcctc gtacggctta tgccccacac cagcgataaa
      481 gacacgccct aacgtataaa cgtcattcga cactgccttt tgtatactcg gccatgctga
      541 aaagagttgt gagtcggggt attcgtcgaa gagggattta tagatgaggg tgccattaaa
      601 atagttatcc cgcaaatcag cttgcacctt tcctgaaaat aaagtcgcat acgctgggag
      661 cgaaaaattt gtaccttgcg aagggataca accttgttgc ttgccacgaa aaaaagtatg
      721 gagtcccaac tcctccatct tttggcgcaa aacttcaatt gtgggacggg cgtccacctc
      781 gcgcacgccg tcgattgcga ttgccacaac gcgcttaacc aacgcacgct cccccgtttc
      841 atagacgggc gagacaaacg tgaacaacaa aaccaagagt tttacacgag gcatcgcttt
      901 ctttattctc gacatttaaa agaaatgaat tgagcatcct caacaacccg acttattaca
      961 aaggtcgtta taacctttga ggatatcggg ttgtttgcga aaaaatgctt cgatagcctt
     1021 atactgaggc gcgttaatgt tgcaacctaa attttttgtc tcagcccagt cgtttttaag
     1081 atcaaacgag cggcatccgc cttcgtcttt gcgtaggatt tgcattttaa tgaaatcgtt
     1141 ggacaccgct gtcaatgtat tttcatttcc ataagtgaat acataccggc gcacgcgctt
     1201 tgtgttgagc gattcccctt gaaactgttc tttcttgtaa gggatgcctg aaacttctaa
     1261 aagcgtgggt acaatgtcgg cgtgcgtcgt ggggtatttt actttgtacg aaatcatata
     1321 gcgcggtgaa tacatcacca agggagatgc gatattctca ttatagcttt ccccagaatg
     1381 cacacggttt ccgggatgtt gataaaacgc ttccccatga tcgccgacga tgataaaaat
     1441 tgtgttattg aacttatcgg tttctttaag ggtggcgata attttttcaa tcacctggtc
     1501 ttgcgcaaag aggttattga tataacggtg gcgagacgaa ttacttttaa cgattttatg
     1561 ttcttcagtt aaatcggggt atggccagtg gccgacaaag gtgtaataaa tacccaaaaa
     1621 aggtttttgg ctttcacgga ttaacgacaa aaaataatca accgacttaa actcatcgcg
     1681 cacgggagtg ggtcccgctt tatactcggg cacatttttc atcgtattgt aatcgtaaat
     1741 acgcgtaaaa cctcggttct taaaccacgc tttggggaaa taccaattaa tatcggcggg
     1801 ggtaaccagg aatgcgtcgt atcgggaacc taaaaaattt attaagtgcg gcacctttaa
     1861 attttccttc atcgaaaaaa atgccgtttg tgggctttca taaagccccg aaaaaatgga
     1921 gaagatggaa cgcggcgaac tgttattcga ggcgaaatgt tgcgacatat acagcgattt
     1981 ttttgtgagc gaatagagat agggcatggg catttttccc tctgcatatt tttcggtatc
     2041 gaaaatatat tcagagccgg tgctctcaag aacgataaaa acaaaattgg ggtattcacg
     2101 gctacggtac aatccttcgc cattatcgat attatttttc gcaaaaagag ggtcatccaa
     2161 atagacgttt gcagtggaga gtgcatctcg tttattcgta tctttttttc tcaaaagcgt
     2221 acgcagaata tcgcgcataa aatattcgtg ggggttcaaa cgcaattcat cgggaatata
     2281 ctgcattgta aaaagcgggc gtaggattaa caccgccgct gcggcgataa gtaccgttgc
     2341 aatatactta cgcgatactc tccagtgttt tctcaaagag agcgcaaaat aaattagcac
     2401 cggagtcagt aaaagtaaaa aatcggttac aggtaacaag gtgacaaaat tatgcagtcc
     2461 taatatggag aagagttcca taaacaaagc atacgtaaac cccgagttca tagtaaaaac
     2521 cgagctatat tgtgaagcag tgataacggt attccagaac acaaaaacag cgaatagaag
     2581 aaggaaaaaa aaaccatgtt ttctcttcaa aacaacgatc gcttcatttt tgaaacgcca
     2641 cgcaaagtgg agtaacaacc agataagatt aaaaacgatg agaacgatac aatcgttgcc
     2701 caatatcaga aaaagggcaa cggcatttga gaatgttttc tccacgccaa aaggagctgc
     2761 ctctgaggca cgcacaccca acgcaagtgc tgtgcggtaa acgagcgcgg tcaatataaa
     2821 gatgaaatta aaaatatgta aaaaacggtt cgagcggtat gcttcaaacg cgatggtaat
     2881 tttgcgaatc acggtaagtc ggcgattaaa attgtcacat cgtctgaaaa cttatcggtt
     2941 ccggaaaact ccaccacctc tcggtaaact tcatcgatga cctgtgcccc atttttttta
     3001 cgtagccgcg tcaaaacctt ctcaaaacgt tcattaccat attcttctct gtcgttattt
     3061 ttttgttcag tgatgccatc ggtaaataaa atgaggcggg tgtcgggcgg gtattgaatt
     3121 ttcttaaact catacggagc aataatcgac gaaccaatta tcgtaccggt tgcctcaatc
     3181 gaccaaaaag aagaagtcgt gctctgcaaa agacagggaa cgtgcccgcc gttagcaaac
     3241 tcgataattc gatttttcgg atcgatgagt aaaatgattc ctgtgagata gtggctcgtc
     3301 gtgacttttt gcatattttg tgaaagccca ttcaaaattt cgcccgcgtt tgcccgcgta
     3361 aagtcctggc tacctaaaga catcttgcac atcgccccca ccaaggcgct cggcacccca
     3421 tgtcctgaaa tatcggcgat gaaaataaaa acacgcccgt cgggtaactc ggcaaaatcg
     3481 taaaggtcgc cagacacctc gtggcatggc ttatagaacg aatggatctg caacgtttta
     3541 agcgaatttt ttgcgggcag cattgcttgt tgaacctcgg ttgccaactc caattcgcgg
     3601 cgggggattt cagcttttgc gcggatttcc ttcgtgcgtt cttcgaccat ttcttccaag
     3661 ttttgagcat actctttaag ctcttggtgc gctttttcca aatctttctc taagttttca
     3721 agtttcttaa aaatatcgag agtgccacgg tttaaattca cattcgtgcg aaaacggcgg
     3781 ataaaaaacg ccaacggaaa cactgagagg aacaaaagcg atgtcttaaa aataatgtgt
     3841 cccgtcagat gactcatgaa atcatgcggg cgggtaagcc ccaaatagat ggccgaatcg
     3901 agcatcatcg tgatccaaag cgcgacaaag ataatgatgc ctaacggtat cttggagaac
     3961 cgattgacca aaaactgaaa taaaataacc gctaagaata gatcgacgat tgtattaaaa
     4021 atcgaaacgc cgatgttgaa cactcgcggt tcaagcactt gatcgacgag cgtccaatcg
     4081 gtaggcgaca ggaatgccgt tttggcttga taaagaaaca cctggaaaac aacgaggaaa
     4141 atttggcatc cgatagaaat aatgatgact ttacgcgctt cgttgattcc ctcggcgata
     4201 tagactaata acagtgcggc aaaaatgaga gtgtaaatcc ccgatgaaaa tcgaaagcga
     4261 taaatggttt cgtcgagttg tatactcgtg gcaaagttga gcccacccgt aaaaaaagaa
     4321 agaaaccaca aaagacaaat ataaaccaaa aacggttcgc ggaagccacg cccacgcaag
     4381 atttgcagtg gccaaagcgg caaaattaac agtaacatgc ttacgaaaaa taagatgagc
     4441 tttgagacat ccacaagcgc actaattgaa ttgagagtct gtgcgccaac tgtaaagtga
     4501 ccaggctgga acttagagcc tgtgcgccaa ctcgccgaaa acccaaaacg atcttgtaac
     4561 cctaacgttg tgtgaaataa ttgcgccttg gttctccgcc gaactcctgt cggcgccgcg
     4621 tcgaaaccgt atttaagcct acaagaaaat actattcaca gccattgata tcatcaaggc
     4681 tcttgagcta tgaagtgttt tcagcgtgat tatttattga ggggaagttt tagaagatac
     4741 attttttcca gtctgcttgt atttttccac aaaagcgctt ggggaacgat aatatccatc
     4801 gtggcaatac aacggtcgag ccaaccttcg gtctttaaca cgtgatcgat agccttgcgt
     4861 aaatctactg ttatcgcctc gccatacgcg ctttccgctt tttcgctggc gagtctgaaa
     4921 gatttcttcg agggcactat cattgccaga tcaataatat ctcgactaaa aacacccgaa
     4981 tcattccagc ggtcagagtt agcgagcaat tttgttgcaa tttggtcgga ttctgacaag
     5041 acgggtactc cacacagtgc ttcagcagca ggtttgcgac gggaaacggc tttttctcaa
     5101 aagccgtcgt ggcgtcaggc gacacgaata gatttcacga cctgcggcag gaccgatttt
     5161 tgccttcatt ttctgcgagt aatactgata cttgaataca gcagcgtcgc tagatatggc
     5221 atatcccgaa agcgatatgc ttttgagcag cttccgcgat cttgcgggga gagagaatgc
     5281 cccgcgaata cgcatcgaaa ataattttaa gaagaatacg cggattgtat gcgggtcaaa
     5341 attggatttc ttaaatctgg gcatggtatc ctccggcaag atattttctt ttctggaaca
     5401 cggattttta ccgaaagcaa gaactttttc tacagtttca acatggttta aatgccgaca
     5461 atataaagaa cctatgcgcc atgttcgtgc aaaaagtatt tttcccggac gagtgcgtgg
     5521 atggatggtc gagttcaagc gcgaacgctt tattgctgaa actcaaattg cacaaaatcc
     5581 acagttggaa tataaaaatt tccgcagcgc tatgcgggcg gcgcagttgc agctcgtcgc
     5641 tgaaaaaaaa attatgctcg atagacgtcg cgaagcgccg catgaaatgg ctgcgctcga
     5701 cgacgccatc gaagttttgg aatcgcatat tcttacttta gaggatcctt attttttagg
     5761 cgaaattaaa aagcgcatat tcacaaccgg tgcaaccgcc gccgctgccg tgggcacagt
     5821 catcggcgcc atatcgcggc agttcatgtt tgattcgtcg agtggtattg tcaacggaca
     5881 aaaccggcac ttcatccaag acatgaacga tatgaaacgc cgcattcttt actttttagg
     5941 tattcaacaa gagttcactc tacctcaaaa cgtatcgatt gtcgcagcgg caaaattaac
     6001 ggtaagcgaa gtgctcgcgc tcaagcgtgc tggcgtcaaa gggattgtca tcggggaaac
     6061 gagtgagacg gcgcatgaaa tgattatgct gcgtgcaatg cgtatcccct gcgtttctgt
     6121 gagtaacttt cgtttttttc gactacgcaa acaagtgccc gcgattatcg acgccgacat
     6181 tggttatatc gttttgcgtc cgcataaaaa tttacgcttt accgaatccc ccaaagaaga
     6241 aacgccgctt tggtcgacga cagtaaaaac aaagtcgggg gagaaagtac agctctctgg
     6301 gacactccat tttacctccg agctcaacac tcttgacgac gcaccgctgc gcgctcaaaa
     6361 aattggtatc tacagaaccg aataccaaat ttctgaggcg ggagaactcc cttctactcg
     6421 ccaactcatt cgccattacg agtatctact gacgcactat cccaaaacag aaatcacatt
     6481 tcgactattc gatttttctg acgataaaaa cttcttaaag agtccgcgta tcgaaaaaaa
     6541 gcccgattta agaggcattc gctttcttct agtcgaaaag aaaattttag aaagccagtt
     6601 acacgcactt atcaaggccg ctgaaagcac acaacgctcc atccgcattc ttgtgcccta
     6661 cctttctgaa acgcatgagt taaattcact gttacacatc atcaatggct ttggtctccc
     6721 taaaaatcaa cccacgattc atctgggcgc aatgttagaa accccggcct caatttttac
     6781 cgctcctcgt tggttaaagg acatcgattt tttctatgtt ggcacctctg atttactaag
     6841 cgcactatcg ctctacaacc gctcggaagc ggaagcgata cagcgtgcgc ttttcagcga
     6901 cgccagcgca gccctcttca accaactcaa aaaattatcg cgtaaaaaac cgctcactct
     6961 ttgcggtgaa attgtgggcg agccgtgggc ggttgcttac ttacgccatt ttgaattcaa
     7021 taactttgtc gtccccgtac accgcatcgc aacgatagta aatatactac aagcgttgac
     7081 gaaagagacg tgcgcgcatt tcgtgcgtca gattacaaaa ctcgacgacg agactaaacg
     7141 gcttgaaaaa gcgcgtgcgc tcattcgcga tattctttac ggtcagtcgt gataaataaa
     7201 gcgtaatcat catgcgcgag ctctatccag aaattcaaaa agtaaaaaca tatacagtga
     7261 aagtttcgcc gctacacacc ctctacgtcg aagagtgtgg caatcccaat ggcattcccg
     7321 ccctttttct acacggcggt ccaggagcgg gactttcgac aaaccaccgc cgctttttta
     7381 atccagaaaa ataccgtatt attcttttcg atcaacgcgg ctcaggcaag agcacgcctt
     7441 atgccgagct gcgtgaaaat acactgtggg atttaatctg cgacatcgaa gtcatccgta
     7501 aaaaactcaa cgtcgcgaaa tggctcgtct tcggtggttc gtggggcagc actctcgcgc
     7561 taacttacgc catccagcac cctgaaagta ttttacatct catcgtgcgc gggatttttc
     7621 tttgccgcca agaagaaatt tcgtggttct atcaatacgg cgcacaccaa attttccccg
     7681 acgcgtggca aaactacgta cgccctattc cacacgaaga acgccacgat atgctcggtg
     7741 cattttatcg gcgtttaacc agcagcgata aaaaaattca actcgaagcc gcgcgtgcgt
     7801 ggagcctttg ggaaggttcg acgatatgcc tacgccccga cctcaataca tcttacgact
     7861 tcaatgaaat cgccgtttcg ctcgctcgga ttgaatgcca ttatttcatg cacaactgtt
     7921 tttatcccga ggataactat atcttaaaca acgcgcataa aattctgcac ttgagcgccg
     7981 acattatcca cgggcgctac gacgtggtat gccctgttaa aaatgcgttt gaccttaaag
     8041 aaaaattacc caaagcaaaa ctcaccgtgg ttgccgacgc aggccacgcc gcgtttgaac
     8101 ccggcatccg cacggcgctc cttgacgcga ccgacgcttt tttattgaac taataccgcg
     8161 ctcttgcctc ggcgagcgca tcgtagagcg cctgccaaaa cgtatcgcgg atgtcggtgt
     8221 tgttgggcaa aagctgcgtc atgagtataa tcaccatttt ttgccgagga tcaatccgat
     8281 aaaacgtgcc atacgcccct gtccaaccat acgattggac ggcgctttgg cccttcgcgt
     8341 tggggtaaag cgttacatca aacccgtagg taaacgtcga cggcatactt gctttttctc
     8401 cataggggga atacgtcatg agtttaactg tgcttttttt caatagcgtt ttatccttcc
     8461 attgaccgcc ctggcgtatc atctccaaaa aatgggcgta gtcaagcgcg gtcgaaacaa
     8521 ggccagcccc acctgaaaaa tttgtgcggt acccttcaaa ataagggtat gtttttttgc
     8581 tcttggatcg ctctgcagtt ccttctttgt tactaccata aagcgcagtc aggcgcgaga
     8641 ttttactctt cggtaagtaa aaatacgtat cgtgcatttc taaagggtta aaaatacgtt
     8701 ccgcaaaaaa tttatcgagt gatttttttg aaactttttc aatcacacac cccaatatat
     8761 cggtgctata tccataacga aatcccccac ccggttgcgc ggctaacggc aaacgcccta
     8821 tctttgccgc cgtttcgcaa atcgtttctt tgagaacgga caaattccaa ttcatcggca
     8881 gtttacgacc caaccctgcg tcacggtaca aatctcccaa ttctttctcc catgcatacg
     8941 aaagtcccga agtgtgcgtg agtagttggt ggattgttat ttcttctttt gctggctcaa
     9001 aactaacggc ctgggttaca gggtctttta caagaacttg catatttttg aattcgggta
     9061 tatattcgct cactttatcg gaaagcttga gtttatgctc ttcaacgagc atcagcgttg
     9121 ccacgctcgt gattgctttc gtcatcgatg cgatacgaaa aatgctgtcc atttgcatcg
     9181 gtactttgac ctcgcggtcg ctaaaaccaa ctgccctctc atagaacggt ttaccatcgc
     9241 gccacaccac tgccaccgct ccgacaactt tattttcagc aacgtacctg ttgagcgttg
     9301 catcgacttt gataaacgct ttttggagct tatcttcgcc acggcagtaa aaaataaaca
     9361 gtaaagcaaa aaccgagcaa acggttcgac tgttaatttt tctacttttt ttcatacgag
     9421 cttccaacca aaggaattga aagcctctgt gtcaattcta aaatttaata aacaagcctt
     9481 ggaattattg acgaggtgtt tcatttttgc acaatgcact atccatggac gctctattta
     9541 tcttgagtct tgtcatctat attattgata tccttgtctt attttactac ggtatccatt
     9601 gttttgtaat ggtacggctt tttttcaagt accgccataa ttgtgagacc gattcttcgc
     9661 gtcttgagga tttgacaaaa aaaatgaaag catggccgcg cgtgaccgtg cagctaccga
     9721 tgtttaacga gtactacgta gccgaacgcc tcatcgatac cacgatgctt ttgaactatc
     9781 ccaaaaacaa acttgaagtg caagtgctcg acgactccac cgacgagacc accgaactcg
     9841 tgcgcaagaa agtaatgtat tacaaaaaaa agggttacga catcaaactc atccaccgcg
     9901 tcaatcgtca aggccataaa gcaggagcat tgcgtgaagc tcaagaaaaa gcaaaaggag
     9961 aatttatcgc tatcttcgac gccgacttca tgcctgcacc cgattttctc gaaaaaaccg
    10021 ttccttattt ttacgaagcc gacgacatcg gcatggtgca aacgcgttgg ggccacatca
    10081 acgccgatta tagcattctc accaaaggcc aaagtctggg gattgatggc cacttcacca
    10141 tcgaacaaat cgcccgtggt ggttctggcc tctggatgaa cttcaacggc actgcgggga
    10201 tttggcggaa gacctgtatc tacgacgcgg gtaactggtc gtcggacact ctcaccgagg
    10261 atttcgattt atcgtaccgc gcagaactca aaggctggcg cttcaaatac gtggtcgatg
    10321 tagtcaatcc ggcagaactc cccgccaccg tttcagcgta caaatcacaa caatttagat
    10381 ggtgtaaggg ctctatccaa accactgtga agcttgcccc caccatttgg cgtaccaact
    10441 taccgtggaa agtcaaactc gaagcgctca cacatctcac caattattta gtccaccctc
    10501 tcatgattat caatatcttg gcgacgctcc ccgttctcta tttcagcgag cggttgggta
    10561 atttacccga cgccattatc ggtgtcgcag cagtattttt ttcgctgggc acctttggcc
    10621 ccatcgcgat gtatttagtg tcgcaaaaag tt
//
LOCUS       DAOMPD010000022        10444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2740_length_10444_cov_3.551641, whole genome shotgun sequence.
ACCESSION   DAOMPD010000022 DAOMPD010000000
VERSION     DAOMPD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2740_length_10444_cov_3.551641"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLY93_01550"
     CDS             <1..613
                     /locus_tag="PLY93_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68198.1"
                     /translation="DIVLCAVGRKPYTEGLNLDKVGVKLTERGRIAVDFHSYATSVPN
                     IYAIGDCIDGAMLAHKAMEEGVACVEVMAGQAGHVNYGAIPGIVYTHPEVAWVGMGEE
                     ELKEKGIEYVVGRSFFKPNGRAKAMMESDGQLKLLSDKKTDRLLGATIVGARASELIA
                     ELTLAMEFGASAEDVARTCHAHPTLSEIIKDAAEQSGGWAIHQ"
     gene            595..4464
                     /gene="cas12a"
                     /locus_tag="PLY93_01555"
     CDS             595..4464
                     /gene="cas12a"
                     /locus_tag="PLY93_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HRP68199.1"
                     /translation="MGDPPVGGSGERNFMTGKKAKTMEQFTNLYSLSKTLRFELKPVG
                     KTLEHIEKKGLLKEDEKRANDYQLVKKIIDRFHKDFMEKALTDLKLAKNDLEDFKRLY
                     SIREKTDAEKEEFDGKKGKGGLKEKLRKQIATQFTKANGWGRLFAKELIKEDLIQIAD
                     NDAEKELIAEFKNFTTYFTGFHENRKNMYTSEEHVTGIAYRLIHENLPKFLDNLNIFE
                     AKIKNNAELKKVFGQLEKELLNNQKITEIFSLNFFNTTLTQTGISFYNTVLGGVAEEG
                     QKKIQGLNEYINLYNQKQDDKNKKIPKLKPLYKQILSDRESSSFQIEAFTEDNELLTA
                     LESFYQTLTVAFEKNGTELNVLSDFRVLATNISEFDGDKIFLRNDLSLTDISQKIFGD
                     WSVIKKALEHYYTSLINPSPQKVTKKYENEKERWLKKSHFSIQEIENAIIHYKDQTDE
                     MKEFKLTGNSQPICDYFKKFETTILVKNDKNENKEVKIDISRNINEKYSDIKDILNTE
                     YNKDKKLSQDKDAVGKIKAFLDAIQELLHFVKPLNPAKVEGEKDEQFYAVYVSLFDEL
                     NNIVSLYNKTRNYLTKKPYSVEKYKLNFENSTLADGWDVNKETDNTAVLLRKDGLYYL
                     AIMDKKHNRLFTEIPKPERDESVFQKVDYKLLPGANKMLPKVFFSEKGQATFNPPASL
                     LEKYKNETHKKGDKFNIADCHALIDFFKKSIVIHEDWKHFGFKFSDTKSYEDLSGFYR
                     EVESQGYKVTFRDVSQNWLNALVAEGKLYLFQIYNKDFSIFSKGRPNLHTMYWKMVFD
                     EKNLKDVVYKLNGEAELFYRKKSLNYDDKTLQEGHHVKELRGKFKYPIISNRRFALDK
                     FQFHVPITMNFKAKGESSNINIDVLKYLKANPDVSIIGIDRGERHLLYLTLVDQKRNI
                     IKQMTLNEIINTYVKDGNTIKVKTDYHEKLNQKEVERAKARESWGVIENIKKLKEGYL
                     SHVVHVIAKMMVEHNAIVVMEDLNFGFKRGRFKVEKQVYQKFERMLIEKLNYLVFKDK
                     KNDDAGGVLKAYQLTNKFESFKKLGKQSGFIFYVPAALTSKIDPKTGFVDYLKPRYES
                     IDKAKSFIKNFESIYYNSKQDWFEFVFNYTDFIPGFKNGKSEWVLCSTHEPRYRWNPK
                     LNQNKGAYEELKITSEFKALFQKHELDFSKGDNLKEKIAAQNDTNFFKELMRLLSTLL
                     SLRHNNGKKGDEERDSIISPVEPFFNSINATEKEPQNADANGAYHIAVKGLWVLNQIN
                     QAKSDDEIRKVKLAISNEEWLNFVQKK"
     gene            4611..4793
                     /locus_tag="PLY93_01560"
     CDS             4611..4793
                     /locus_tag="PLY93_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68200.1"
                     /translation="MIEQEMQVSFETALRELYNSRLFQDLADEPTKIWHFSDYLLLDL
                     FKQEKLTGKIDYPNVF"
     gene            5058..5618
                     /gene="cas4"
                     /locus_tag="PLY93_01565"
     CDS             5058..5618
                     /gene="cas4"
                     /locus_tag="PLY93_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HRP68201.1"
                     /translation="MEPYIPISFLNDFIFCPRSIYFHQLYGRTDTRYYQAKAQVAGKA
                     AHESIDNQTYSTRRDVLQGYEIFCEKYNLCGKLDTFDIKSGRLTERKREIQKIYDGYV
                     FQVYAQYFALCEMGYTVKEIVIYDITHNKNYPIPLPNDDAIMLKKFEQLVYDINHFDL
                     DKKDFVPNEAKCKCCIYNNLCDYSLA"
     gene            5656..6621
                     /gene="cas1"
                     /locus_tag="PLY93_01570"
     CDS             5656..6621
                     /gene="cas1"
                     /locus_tag="PLY93_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated endonuclease Cas1"
                     /protein_id="HRP68202.1"
                     /translation="MLGLPDFREKSIVICFASDGQKVAFKNDNLVVKDENDKIVLQIT
                     CHRIFSLWIIGRTIVTSGLLERSKKFAFSVYLLSYSHRLYGLWSSSVEGNFLLRRKQY
                     AYEGVDIAKHLVQNKIVNQSALLKKLRTKTVEIKEAIEKMNGYAKQVNEMQDLQDLLG
                     IEGVASRLFFGHWYGDLGWRGRKPRTKIDAINALLDIGYTYLFNLVECMLNLYGFDLY
                     QGFYHRCFYQRKSLVCDIVEPFRCIVDRQVRKAYKLKQFQFEDFEERKGQYFLKIEKN
                     KEYTRWLVQGLLEHKEAIFLYTQDFYRSFMKNRPISEYPVFRLDG"
     gene            6624..6896
                     /gene="cas2"
                     /locus_tag="PLY93_01575"
     CDS             6624..6896
                     /gene="cas2"
                     /locus_tag="PLY93_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRP68203.1"
                     /translation="MLLVSYDISEDKLRTKFAKYLSKFGARLQYSLFEIKNSDRVLQV
                     ISVDIENLFGKQFGETDSVMVFDLSKQCKITRFGYAKHDETDLIIF"
     repeat_region   7111..9727
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7175..7210
                     /rpt_unit_seq="gtctaagacaccctataaatttctactattgtagat"
     gene            complement(9798..>10444)
                     /locus_tag="PLY93_01580"
     CDS             complement(9798..>10444)
                     /locus_tag="PLY93_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRP68204.1"
                     /translation="HGYEGQGPEHSSARIERFLQLCAKNNMSVLNLTTPAQMYHALVR
                     QALDKQKKPLVIMTPKSLLRHPQAVSTLEDITQGRFNEILDDSAVKKGDVTRILLCSG
                     KVYYDLLKERDARKLKHISIVRMEQLYPLKYDAVADLLARYTNLRELIWVQEEPKNMG
                     AWFYIKDRFDERLADGKYGTMLRSVARKTSPSPAAGLMKVHEREQKELIDRALL"
BASE COUNT         3361 a         1897 c         2108 g         3078 t
ORIGIN      
        1 cgacattgtt ctctgcgcgg tggggcgtaa accgtacacc gaaggcttaa atctcgacaa
       61 agtgggcgtc aaactcaccg agcgtggtcg catcgctgtg gatttccata gctatgcaac
      121 gagcgtaccc aatatatacg ccattggcga ctgtatcgac ggcgcaatgc tcgcacacaa
      181 agcgatggaa gaaggcgtcg cgtgcgtcga ggtgatggca ggccaagccg gtcacgtcaa
      241 ctatggcgca atccccggca ttgtgtacac acaccccgaa gtggcgtggg ttggcatggg
      301 tgaagaagag ctcaaagaaa agggcatcga atacgttgtt ggtcgttcat tcttcaaacc
      361 caacggccgc gccaaagcga tgatggaaag cgatggtcaa ctcaaattgt tatccgacaa
      421 aaagaccgat cgcctgttgg gagcgaccat cgtcggcgca cgcgcatccg aacttatcgc
      481 cgagctcacc ctcgcgatgg agtttggagc ctctgccgaa gacgtggcgc gtacctgcca
      541 cgcgcatccg acgctctcag aaatcatcaa agacgctgcc gagcaaagcg gtggttgggc
      601 gatccaccag taggtggtag cggggagagg aactttatga ccggcaagaa agcaaagaca
      661 atggaacaat ttacgaattt atattcgtta agcaagacac tacggttcga gctaaagccg
      721 gtggggaaaa cgctcgagca tattgaaaag aaaggcttac tcaaagaaga tgaaaagcgg
      781 gcgaacgatt atcaacttgt gaagaaaatt atagaccgct ttcataaaga ctttatggaa
      841 aaggctttga cggatctaaa gcttgcaaaa aatgatttag aagatttcaa acgcttatac
      901 tctatacgag aaaaaacgga tgcagaaaaa gaagagtttg acggtaaaaa aggtaaaggt
      961 ggcttaaaag aaaagttgcg taagcaaata gctacgcagt ttacaaaagc aaatggttgg
     1021 ggacgccttt ttgcaaaaga acttattaaa gaagatttaa ttcaaattgc tgataacgac
     1081 gctgaaaaag aacttattgc cgaatttaaa aatttcacca cttattttac aggatttcac
     1141 gaaaatcgta aaaatatgta cactagtgaa gagcatgtta ctggcatagc ctatcggctc
     1201 attcatgaaa atttacctaa gttcttagat aatttaaaca tttttgaggc caaaataaaa
     1261 aataatgcag agctaaaaaa agtctttggg cagttagaaa aagagctttt aaacaatcaa
     1321 aaaataactg aaattttttc actgaacttc tttaatacaa cactgactca aacaggtatt
     1381 agtttttaca atacagtttt aggtggcgtt gctgaagagg ggcaaaagaa gattcaaggc
     1441 ttaaatgaat acatcaattt gtataatcaa aagcaagatg ataaaaacaa gaaaataccc
     1501 aaattaaaac cgctttacaa gcaaattttg agcgatcgtg aaagctcgtc gtttcaaatt
     1561 gaagcattta ccgaagacaa cgagctctta acggcgctgg agagttttta tcaaactttg
     1621 actgtcgctt ttgaaaaaaa tggcacagaa ttaaatgtgc tatctgattt tagagtttta
     1681 gcaacaaata tttctgaatt tgatggcgat aaaatttttc ttagaaatga tttatcctta
     1741 acagatatat ctcaaaaaat ttttggcgat tggagtgtca taaaaaaggc attagagcat
     1801 tactatacca gtctaataaa tccatcacca caaaaagtta caaaaaaata tgaaaatgag
     1861 aaagagcgtt ggctcaaaaa atctcatttt tcgattcaag aaattgaaaa tgcgattatt
     1921 cactataaag atcaaaccga tgaaatgaaa gaattcaagc ttactggtaa tagtcagcct
     1981 atatgtgatt attttaaaaa atttgaaaca acgattttgg ttaagaatga caaaaatgag
     2041 aataaagagg ttaaaattga tatttcaaga aatatcaatg aaaaatacag cgacataaaa
     2101 gatatactga acactgaata taataaagat aaaaaacttt cacaagacaa agatgcagta
     2161 ggtaaaataa aagcgttttt agatgcaatt caagaattat tgcattttgt taagccgtta
     2221 aacccagcta aagtagaggg ggaaaaggat gaacaatttt atgctgtgta tgtatcttta
     2281 tttgatgaac taaataatat tgtatcgctc tataacaaaa cccgaaatta cttaacgaaa
     2341 aaaccttatt cggttgaaaa atataaactg aattttgaaa atagcacttt ggctgatggg
     2401 tgggacgtta ataaagaaac tgacaataca gcggttcttt tgcgaaaaga tggtctttat
     2461 tatttagcta taatggataa aaagcacaat cgacttttta ctgaaatacc taaacccgaa
     2521 agagatgagt ctgtatttca gaaagttgat tataagcttc taccaggagc taataaaatg
     2581 ctgcctaaag tctttttttc tgaaaaaggc caagcaacat ttaatccacc tgcgtcactt
     2641 ttagaaaaat acaagaatga gacacacaaa aaaggagata aatttaatat agcagattgt
     2701 catgctctta ttgatttttt taaaaaatca attgtcattc atgaagattg gaaacatttt
     2761 ggctttaaat tttcagatac caaatcttat gaagatttaa gcggctttta tcgagaggtt
     2821 gagtctcagg ggtataaagt tacttttcgc gatgtttctc aaaactggct taatgctttg
     2881 gttgctgaag gtaaactcta tctgtttcaa atttataata aagacttttc aattttcagt
     2941 aaaggtcgac caaacttaca cactatgtat tggaaaatgg tttttgatga aaaaaatcta
     3001 aaagatgtag tgtataagct taatggtgag gctgaacttt tttaccgtaa aaaatcgctc
     3061 aattacgatg acaaaacatt gcaagaaggc catcatgtaa aagagcttcg gggcaaattt
     3121 aaatatccaa tcattagtaa ccgccgtttc gcattggata agtttcagtt tcatgtaccg
     3181 attaccatga acttcaaagc taaaggcgag agcagtaata ttaacattga tgtcttaaaa
     3241 tatttgaaag cgaaccccga tgttagcatc attggcatag accgtggtga gcgtcattta
     3301 ctttatttaa cattagttga ccaaaaacgt aatatcatca agcaaatgac attgaatgag
     3361 attataaata cttatgttaa agacggtaat acaatcaagg tgaaaacaga ctatcatgaa
     3421 aaactcaacc agaaagaagt tgagcgggcc aaggcacgcg agagctgggg agttattgaa
     3481 aatataaaaa aactcaaaga aggttatttg agtcatgttg ttcatgtgat agccaaaatg
     3541 atggttgagc ataatgccat tgtcgtcatg gaagacctga attttggttt taagcgtggt
     3601 cgattcaagg ttgaaaagca agtttatcaa aaatttgaga gaatgctaat tgaaaagctg
     3661 aactatcttg tcttcaaaga taaaaaaaat gatgatgcag gcggcgtgct taaggcatat
     3721 caactgacca acaagtttga aagttttaag aagttaggta aacaaagtgg atttattttt
     3781 tatgtgccgg cggctcttac tagtaaaata gaccctaaaa ccggctttgt cgattactta
     3841 aaaccccgct atgaaagtat tgataaggct aaaagtttta ttaaaaattt tgaaagtatt
     3901 tactataatt caaaacaaga ttggtttgaa tttgttttta actacacaga ctttatacca
     3961 ggttttaaaa atggtaaatc ggagtgggtg ttatgtagta cgcatgaacc tcgttaccgt
     4021 tggaatccta aacttaatca aaacaaaggg gcgtatgaag agttaaagat tacatctgaa
     4081 ttcaaggcgt tatttcaaaa gcatgagtta gatttttcta aaggcgataa tctcaaagaa
     4141 aaaatagccg cacaaaacga tacaaatttt ttcaaagaac tcatgcgatt gctttcgact
     4201 ttgctctctc tgcggcataa taatggtaaa aagggtgacg aagaacgaga ttcaattatt
     4261 tcacctgtag agccgttttt taattcaata aatgccaccg agaaagagcc gcaaaatgca
     4321 gacgcgaacg gtgcttacca cattgccgtg aagggacttt gggtattaaa tcagattaac
     4381 caagcaaaaa gtgatgacga aatacgaaag gtaaaattgg ctatcagtaa tgaagagtgg
     4441 ttgaattttg ttcaaaaaaa ataatccacg atgacacttt atcacgcaag caatgtagca
     4501 gttcaaaaac caattatcat tgatagcttg cgtggccttg atttattatg acgagtgtag
     4561 aagaattgta tcgagtttca ctgcggttga agctgcctcg tttggttagt ttgattgaac
     4621 aagaaatgca ggttagtttt gaaacggcac ttcgtgagct ttataactca cggctttttc
     4681 aagacttggc agacgagccg acaaagattt ggcatttttc agattattta ctgctggatt
     4741 tgtttaagca agaaaaattg actggtaaaa tagattatcc caatgttttc taatagcggc
     4801 atatcacaag ctcaaaagga tttcgcagtg ttctgcgttg aagagtaccg aacgcggcat
     4861 gggttatcgg gaaaaattgt gcatgaaatt tttgataaga cggggctact cgattacgtg
     4921 attgactgtt atgatgtttt gcattcgttc ggtgtcgaat atttaatgga tgattttcaa
     4981 gactatctaa aaaatcattc acaaaaactt actttgttgt caagtgaggc acggcgagcg
     5041 gtcgatgctt atagttagtg gagccgtata tacccatatc gtttttgaac gattttatat
     5101 tctgcccgcg ttctatttac tttcatcaac tttatggtcg taccgacact cggtattatc
     5161 aagcaaaagc tcaagttgcg ggtaaggcgg cgcatgaaag tattgataat caaacttatt
     5221 cgacgcggcg tgatgtattg caggggtatg agatattctg tgaaaaatat aatctttgtg
     5281 gtaaactcga tacctttgat ataaaaagcg gcaggcttac cgagagaaaa cgcgagatac
     5341 aaaaaattta cgacggttat gtctttcagg tttacgctca atatttcgct ctttgtgaaa
     5401 tgggttacac agtaaaagaa attgttatat acgatattac gcacaataag aattacccga
     5461 taccgttgcc gaatgatgac gcgataatgc tcaaaaaatt tgagcagctt gtttacgata
     5521 taaaccattt tgatctagat aaaaaagatt ttgtgccgaa tgaagccaaa tgcaaatgct
     5581 gcatttataa taatttatgc gattatagtt tggcatgaat tttgccgatg tgtaaaatgt
     5641 tttgcaaggt atgctatgtt ggggttacct gatttcaggg agaaaagcat tgttatctgc
     5701 ttcgccagtg acgggcaaaa agtagcattc aaaaatgata atcttgtcgt caaagacgag
     5761 aatgataaaa tagttcttca aataacctgc catcgtatat tttcactgtg gattatcggg
     5821 cgcacaatcg ttacttcggg tttgttagag cgcagtaaaa aatttgcttt ttcggtctat
     5881 ctcctttctt acagccatcg tctttacggt ttatggtcaa gtagtgttga gggtaatttt
     5941 ctattgcgcc gaaaacaata tgcatacgaa ggtgtcgata ttgccaagca cttagtacaa
     6001 aataaaatcg taaatcaatc cgccttgttg aaaaagctgc gcacgaagac tgtggagatt
     6061 aaagaagcca tagaaaaaat gaatggctac gcaaagcagg tcaatgagat gcaagaccta
     6121 caagacttgt tgggtataga aggcgttgcc agtcgtttat tttttgggca ttggtatggc
     6181 gacttggggt ggcgcggtag aaagccgcgg acaaaaattg acgctatcaa cgcattgctt
     6241 gatattggtt atacgtatct ttttaatttg gtcgagtgca tgcttaacct ttacgggttt
     6301 gatttgtatc agggatttta ccaccgttgt ttttatcaaa gaaaatcttt ggtctgcgat
     6361 attgtcgaac cgtttcgttg tattgtcgat cgtcaggtaa gaaaggcgta taaattgaaa
     6421 caattccagt ttgaagattt tgaggagcgc aaagggcagt attttttgaa aattgaaaaa
     6481 aataaggaat atacacgctg gttggttcaa ggattgcttg aacacaaaga agctattttt
     6541 ctttatacgc aagattttta tcgaagcttt atgaaaaata gaccgataag cgaataccct
     6601 gttttcaggc ttgacgggtg aaaatgcttt tagtctctta tgatatatca gaggataagc
     6661 tgcgcactaa gtttgcaaag tatttgagca agtttggggc aagattgcag tattcgctct
     6721 ttgaaataaa aaatagtgat cgcgtgcttc aagttattag cgttgacatt gagaatttat
     6781 ttggtaagca atttggtgaa accgatagtg ttatggtttt tgatttgagc aagcagtgca
     6841 agattacacg ttttggttat gcgaagcacg acgaaaccga cctaatcatt ttttgagtta
     6901 agggggcgag ttattcttag ttgcaaacct cagtcttaat gcagacttgg cttgctaaat
     6961 gtataaagta gggttcagaa gacaaagttt ttggccaaaa aaggggcaaa aaggtggatt
     7021 ttccgctcaa aaagcccttt gtgaggatgt ttggagacag ttatctgtct aagacaccct
     7081 ataaatttct actattgtag atccgaaaac gcagaatcgg caagcatctt gtctactatt
     7141 gtagatcagg atcctattga tctgaattat ttgagtctaa gacaccctat aaatttctac
     7201 tattgtagat cagcggtatc atatcaccgt gagtatgtct aagacaccct ataaatttct
     7261 actattgtag atctcgctca atggagagca ctgggtcaag gtctaagaca ccctataaat
     7321 ttctactatt gtagattttc cgctttttcg agtgctgcgg aagtctaaga caccctataa
     7381 atttctacta ttgtagatag tggcgaaaaa ttccctgacg cagtacgtct aagacaccct
     7441 ataaatttct actattgtag atagcctgtc aatcaaacct gccaaggcgg tctaagacac
     7501 cctataaatt tctactattg tagatgacct cgtttttttg tccttgtttt tggtctaaga
     7561 caccctataa atttctacta ttgtagattc cccgccttca cggtgaaatt tctccgtcta
     7621 agacacccta taaatttcta ctattgtaga tatccgtcgg tcgaacaata tatttcgagt
     7681 ctaagacacc ctataaattt ctactattgt agatagaaaa accaacctga gcattcgcaa
     7741 agtctaagac accctataaa tttctactat tgtagatgtt cgcggtattc gacaccgcgt
     7801 aacgtctaag acaccctata aatttctact attgtagata ctcaataaaa aaatcgtcga
     7861 ggggcaaggt ctaagacacc ctataaattt ctactattgt agatacatca cttactttcc
     7921 aaaaatcctt tgtctaagac accctataaa tttctactat tgtagataac gacgtcgact
     7981 tcatactgtc cgccggtcta agacacccta taaatttcta ctattgtaga taaaaatcgc
     8041 gtcggctatc tcgtggtgtc taagacaccc tataaatttc tactattgta gatgagaatg
     8101 gcctttgctt cttcgtcacg gtctaagaca ccctataaat ttctactatt gtagatggtg
     8161 gtagtcgaga tactatacaa gtggagtcta agacacccta taaatttcta ctattgtaga
     8221 tatgggcatg gactgggtcg cctctgagtc taagacaccc tataaatttc tactattgta
     8281 gatcacagca tcgtgagagt cttcgtcgag tctaagacac cctataaatt tctactattg
     8341 tagataagag gtgggttcgg agagcgagta aggtctaaga caccctataa atttctacta
     8401 ttgtagatga gtatgttttc tggctcgccc cacgtgtcta agacacccta taaatttcta
     8461 ctattgtaga ttcatattct tcttgcggcg tttgtaggtc taagacaccc tataaatttc
     8521 tactattgta gataatttca ttttttgacg cattaccaga gtctaagaca ccctataaat
     8581 ttctactatt gtagatcgct gtcttttttg tgttcgactc aatgtctaag acaccctata
     8641 aatttctact attgtagatg tgcaccgccg acaagcggcg caatgggtct aagacaccct
     8701 ataaatttct actattgtag ataatttcat tttttgacgc attaccagag tctaagacac
     8761 cctataaatt tctactattg tagatgtaat cttgagtacc ttggacatat cgtctaagac
     8821 accctataaa tttctactat tgtagattcg gacaacaacc gccacagctt gccgagtcta
     8881 agacacccta taaatttcta ctattgtaga tgcggcgtag agcttcttgt gactctgtct
     8941 aagacaccct ataaatttct actattgtag atgattatga gcgataatag agtgaaactg
     9001 tctaagacac cctataaatt tctactattg tagattagaa tgcgcacccg aacttttgcc
     9061 aggtctaaga caccctataa atttctacta ttgtagatgt attctgctgt tttattttta
     9121 gcattgtcta agacacccta taaatttcta ctattgtaga taaatcggga gtaccactaa
     9181 atgtaacgtc taagacaccc tataaatttc tactattgta gataagacac gtcaagtgac
     9241 agaactgaga ggtctaagac accctataaa tttctactat tgtagatgcg atgtccccgc
     9301 cgatgatgag tcgggtctaa gacaccctat aaatttctac tattgtagat gaaaactatt
     9361 cgggacttga atcgtggtct aagacaccct ataaatttct actattgtag atcttacggt
     9421 tgtcgatggg gcacgaacgg tctaagacac cctataaatt tctactattg tagatggcgg
     9481 tggttagaac agttgcaacc aggtctaaga caccctataa atttctacta ttgtagatga
     9541 gagcatttgc cctgcaggta aatgtgtcta agacacccta taaatttcta ctattgtaga
     9601 tctgtgtagc ctgcaccata accacggccg tctaagacac cctataaatt tctactattg
     9661 tagataaaaa ttaagtgagc aaacgcgagt agtctaagac accctataaa tttctactat
     9721 tgtagatggg agattcgtag gcgccgggct ctgctcccga aaaaaaactg cggctcggca
     9781 acccaccact aaccggacta caacagcgcg cgatctatca gctctttttg ctcgcgttca
     9841 tgcactttca tgagacccgc tgccggtgaa ggacttgtct tgcgcgccac cgagcgcaac
     9901 attgtgccgt acttgccgtc ggctaaacgt tcatcgaagc gatccttaat gtaaaaccag
     9961 gcgcccatat ttttgggttc ttcttgcacc caaatcaact cgcgtaggtt ggtatagcgg
    10021 gcgagtaaat ccgcaacggc atcgtatttt agcgggtaaa gttgctccat ccgcacgatg
    10081 ctaatgtgtt ttaatttgcg tgcgtcgcgc tctttgagta aatcataata cactttaccg
    10141 ctgcacaaaa ggatacgggt gacatccccc ttcttgaccg cgctatcgtc caaaatttca
    10201 ttgaacctcc cttgtgtaat gtcttctaat gtgctcaccg cttgagggtg ccgtaaaaga
    10261 cttttgggcg tcataattac caaaggcttt ttttgtttat ccaacgcttg ccgcacaaga
    10321 gcatggtaca tctgcgcagg cgttgttaaa ttcaacacgc tcatgttatt ttttgcacaa
    10381 agctgcaaga aacgctcgat gcgcgcgctc gagtgctccg gcccttgacc ttcataaccg
    10441 tgcg
//
LOCUS       DAOMPD010000023        10403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2756_length_10403_cov_3.515559, whole genome shotgun sequence.
ACCESSION   DAOMPD010000023 DAOMPD010000000
VERSION     DAOMPD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_10403_cov_3.515559"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLY93_01585"
     CDS             <1..259
                     /locus_tag="PLY93_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRP68205.1"
                     /translation="PVDSEAILRAAQKTHRLLILQEGTPVASFGSWLSHQIHEIAFDE
                     LDAPVKLISSDDVPMPYTFSLEPEVLPSVEKVVNAVKELIR"
     gene            complement(312..863)
                     /gene="def"
                     /locus_tag="PLY93_01590"
     CDS             complement(312..863)
                     /gene="def"
                     /locus_tag="PLY93_01590"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRP68206.1"
                     /translation="MAVKRVLTIQDERLYQVSEKITRFDKSLRDLVRDMFDSMAAEGG
                     VGLAAVQIGVLKRVLVIDLIEKGFLKGAFINPHLIEASQEMQDGEEGCLSVPGLSAEL
                     KRPRWVKIGYQDLSGNEKTVEGEMLLARAFLHEMDHLDGKVFVDQLEPKLHKQLMGDI
                     DRIKQGLSVKEPHLPKYRNKKAS"
     gene            1020..3497
                     /locus_tag="PLY93_01595"
     CDS             1020..3497
                     /locus_tag="PLY93_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68207.1"
                     /translation="MQSIAERGGANQQIPKGQLVVAKGHIVRSLIILQKGQVSINAVE
                     VGPMGQAIQRPLFNINAPCVLAGASLFSGTRQNFQITTSTTCEISVYPSNKEYLLKLI
                     QAKPNIGVLALKSVYKELTDIETKLEQAEQTWEQLSAFELGFSLSLNALLPEKFDDTD
                     KATGFTDPLLPIARAQITDFTERGGSIVKPLNINFLRSARNQIETSEEKKNDNSTIAL
                     VRRLVSLPQDALSLIASRDAQLFYAAAESIVDNLYAKNAALDAIFLKIQEYITRLLLG
                     DYAWLSKFALQAELLESNSNTASREDIIATCMFYSEAFSAVRDKLLTFAEITYPPFVQ
                     TALQKIQKVAAQKEATKPVVAAEQGASLSSDMSEFQNALETILLWCDIPEDKKQQFKK
                     DWDALKKFKNPLDGSDDDIRKLRRRVGVQYWEIFERGMLKYLRKRTDLPRVVRLFFDY
                     GFMDETQLDSEQLTTLANASYGKGESWLPVYTVTEWLAAIYDNKVRTSISEMGLTFFE
                     MLRQDPSNRDKKWKRESDLPPEVNSPEARVKFEIKEMLQMNCRLCSGNISSHLNILTR
                     HQITQALPKVMITKDTVVDQIKKILAVDFGAFHREILYTNEQMNIQREFIQVQVMPNI
                     ILVPSIGTVIQFWQEREGNDRTSRGRLIAPIMVTADLHTMLLKAVGTYRWEMCKTTAG
                     PDWNNISVSSLTADYTDYIQFYYKSKELSEEIKEKLSEDFKRFRDDRARFVNDYTIYM
                     RFESDGSQRMNRVSRKIFTKHIPFAKEIREKLLKLPSYTDVVQKSINIRKRKANELTP
                     RYTKYERENKTLPKELVETRKFYNMEY"
     gene            3514..3960
                     /locus_tag="PLY93_01600"
     CDS             3514..3960
                     /locus_tag="PLY93_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRP68208.1"
                     /translation="MRFCAAYTLLSLGVNAVYANSIVISGKIYSENNNAPIEFGTVVI
                     PEAKFKSRVQADGRYAMSVPNAGTYTIIITSPGLKTIQEKITITKSTTRDFRMELPIV
                     KTRTVRLRAERDIQKLGRNTLSVEQLKETRNIRRRVKRTGYATRRR"
     gene            3935..5953
                     /locus_tag="PLY93_01605"
     CDS             3935..5953
                     /locus_tag="PLY93_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRP68209.1"
                     /translation="MATLPGVVRPGGFFGPLVIRGAGDKANRYFIDDIPVPNPQHFGG
                     LQSIISNDLMREIDLYASAFPSQFGGALGAIIDIQTIDEVKELGGVIDVSLISANYLV
                     KNTWGKNYGGETTVTSAEKGDNTTEETSAKNSSAKGYWISSGRIGYLSLFVPPIYKLI
                     TGKSLDRLPQYYDYQLKGKVFLDTKGRHALSLLAFGSYDTLKFVRNLSEEEKAKRKAE
                     GDDPFLAGFNLSNDITSHSQGLYYDYIPSAQISNRLILFNSFTYSYFYANFTDTRTIG
                     PIDLKIYPSILGIKDKTKWEWASFATLRTALEYNLYYFKSMGQTQQQLNVSAGGPPDY
                     GNLAGFASVPVDFSTTNHLVSSYIENKFQFGGWNFVPGVRGEFLSRNRSAIIDPRALI
                     GYTFDSDTTVSAAGGQYQSFSQTNTFYFNQVFNYQPQVVVADYIKPERAFHRTLGIEQ
                     KIVNYTLKIEGFLNDFDRLLEAANNGQVTGKYFSNNGALKARGVEVIIRKDSEEKEFD
                     YYGWVSYTYTQGERLRYGAWGKYEFEQPHSLKLVGGIKWRAHTLGAQFQLFSGYPYTN
                     ITGSTCTTGFDCSSPSTARYSPLYSSNSYDGHFTASHRLDIRYTHKTAYKWGYFSWYI
                     EVINIYNQQAANQLKWNYNEPYSSSNPKIQIPDGIITIIPYFGLEWRF"
     gene            complement(5958..8069)
                     /locus_tag="PLY93_01610"
     CDS             complement(5958..8069)
                     /locus_tag="PLY93_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68210.1"
                     /translation="MVRVYIFVILAFALALGAYIAERFVLVNRATALSEVRFDSLRSD
                     IERIDQEINERNRQGEFFRYLGVRPHGNKFDQREKEVRELNPNHDLIESALVLTNHFK
                     TLVRSGRDLSLQERTLLSTQLTDTLLADTGLLRRYNITDFTGKRLGYAVFIIRPDLKK
                     STTALFVTSYDFETVAAFSHDAFNAEEKKLVEGVLSRLTSNREAFSIRGREYVALKQV
                     WLDANVYLFQVSPLPPIYTYWSIYFTLILFIVVFADILRVYRAKVMTRREVSGRILDN
                     HRRALAMHKQTFETLAKLNDENVEKEINANFLSAQPSDPILAVEEKIRLGRAHFVAKE
                     PPLQTEITIETNTEKRQFRFMNPALTEKASAPKILQLNEREEKIRGKAFSDELKTLMT
                     EVASPITKEAVKSVTTADYLLRIEQFENAHRYPSVDQYLYYLNELYFDDVTADELFEA
                     MRVASDGVQASAFAIMIYEPLNAVYRVGLSHDVPQNLKDYFYLLLKDSVFSIASDEYT
                     YIEFSAALRKNPFFTKRFPKGLTEKFKGIHVFSLNESFLKARLVFFDDTRGGALDDFE
                     LISNTKSYLKQIAPAIKMFFIEAYENKGDVFRLDNGAVRELKESLALIDCDAPLIFQY
                     VFERALSVQESTLLIQKIAAQLSNYCKAILLSPSHFIVLQTPAVSSTVEEVIRECGVE
                     FLVKESTFGHKTHNLFAFIEF"
     gene            complement(8076..8543)
                     /locus_tag="PLY93_01615"
     CDS             complement(8076..8543)
                     /locus_tag="PLY93_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRP68211.1"
                     /translation="MSNANFFSARPTIRHRIAGIFMRGDDILLVKHRKAGREYYLLPG
                     GGQELGESAIDALKREWYEELNLQVQVGKFLFCGESVPSEAKRTQVFQMVFQIDAIDG
                     RIEVKREGALAGYAWIPLGRIDKVAFFPDCLPQVKAFCSGKKFESYQRYRWLT"
     gene            complement(8540..9046)
                     /locus_tag="PLY93_01620"
     CDS             complement(8540..9046)
                     /locus_tag="PLY93_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRP68212.1"
                     /translation="MRRYLLRHDQTVLSRIVSNNQFTVGTAWNIKKALPFAVTHCDLK
                     KRVMTVRADGKEHIAHFHQTNDGIELVLDGFFYRFTNTPFSGSDSEKVHHSEIRSEIP
                     GRIVKICAAVDQVVEQGTALIIQEAMKMEITLVAPARVKIQEVLVEAGSQVDADVVLI
                     RFEAAPLS"
     gene            complement(9210..9896)
                     /locus_tag="PLY93_01625"
     CDS             complement(9210..9896)
                     /locus_tag="PLY93_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRP68213.1"
                     /translation="MSQATNSSPRFFSTSIGKKFLMALSGLFLVSFLPIHLAGNLLLF
                     KDDGGAAFNAYSLFMSTSIIIRVLEVTLLLGFLVHIIDGIILTRQNKAARPVGYSGGK
                     SYVKSFFSRYMDLTGLVILVYLIIHINSYTLKHRIYEPGNLAFYETVKASFETGWGGF
                     YPWFYVLAMLLISFHLNHGFQSAFQSLGLNHKKYTPFIKKLGLLYSIVIPLGFAAIPI
                     FFQLKYFNVI"
BASE COUNT         3043 a         2320 c         2218 g         2822 t
ORIGIN      
        1 gcctgtggat tctgaggcca tcttgcgtgc tgcccaaaaa acccaccgct tactcatttt
       61 gcaagaaggc actccggtgg cgtctttcgg atcgtggctt tcgcatcaaa tccacgaaat
      121 tgctttcgac gaactcgacg cgccggttaa acttatttct tctgacgacg taccgatgcc
      181 gtacacattt tctctggaac cggaagtatt acccagcgta gagaaggtcg tcaacgcagt
      241 gaaagaacta attcgctgaa atttaccacg cagcgagcgc gcgataaaat attctgcgtg
      301 tacgctttct attaagacgc ttttttattt ctatatttgg gtaaatgtgg ttcttttacc
      361 gacaaccctt gcttgatgcg gtctatatcc cccatgagct gtttatgcaa ctttggttct
      421 aattgatcaa caaacacttt gccatctaag tgatccatct cgtgtaaaaa agcgcgggcg
      481 agcaacatct cgccctcaac agttttttca ttgccgctta aatcttgata accaattttt
      541 acccaacgtg ggcgcttcaa ttctgccgaa agacctggta cagagaggca tccctcttcg
      601 ccgtcttgca tttcttgcga ggcttcaatc aaatgaggat tgatgaacgc acctttgaga
      661 aagccctttt cgattaaatc aatcacaagc actcgtttca agacaccgat ttgcacggca
      721 gccaaaccaa cgccgccttc ggcagccatt gaatcgaaca tatcccgcac caaatcgcgc
      781 agagatttat caaatcgggt aattttttcg gaaacttgat aaagtcgttc gtcttgtata
      841 gtgagaactc gtttgacagc cattggggta aatatagcaa aaattaaatc ggattgctct
      901 tttttttagc tgggcgaatt tttgtcccaa tgagttgccg cacaggctcg taaataaccc
      961 aatcgggtag tacattcgcc ttgacgggtt cgatacgaat ttagtatttg tgaaaatcaa
     1021 tgcagtctat cgctgagcgt gggggcgcaa atcaacaaat acccaaaggc caacttgttg
     1081 tagccaaagg ccatattgta cggtctctta tcattttgca gaaagggcaa gtctctatca
     1141 atgccgtgga agtcggacct atggggcaag caatccagcg cccacttttt aatatcaatg
     1201 caccttgtgt attggcaggg gcgtcgttat tttccggtac acgtcaaaat tttcaaataa
     1261 ctacatccac tacttgcgag atcagcgttt accctagcaa caaagaatac ttgttgaaac
     1321 tcattcaagc gaaacccaat atcggagtgc tcgcgttaaa atcggtatat aaagaactta
     1381 ccgatatcga aacaaaattg gaacaagccg aacaaacgtg ggagcaatta tccgcgtttg
     1441 aattaggttt ttctttatcc ctgaatgcgc tcttgcccga gaagtttgat gacaccgaca
     1501 aagcgacggg cttcacagac cccttgttac caatcgcacg cgcgcagatc accgatttta
     1561 cggaacgggg tggcagtatt gttaagccac tcaacatcaa ttttttacgt tctgctcgta
     1621 atcaaattga aaccagcgaa gagaaaaaaa atgacaactc gacgattgca ctcgttcgtc
     1681 gtctggtgtc gctaccacaa gacgcattaa gtctcattgc atcgcgcgat gcacagcttt
     1741 tttacgcagc agccgaatcc atcgtcgata acttgtatgc taaaaatgcc gctcttgacg
     1801 caatcttttt gaaaatacag gaatatataa cccgtcttct tttaggggat tacgcgtggt
     1861 tgtcgaagtt cgcactacag gccgaacttt tagaatcgaa ttcaaacacg gcaagccgcg
     1921 aagatattat tgctacgtgc atgttttata gtgaagcgtt ttctgcggta cgcgacaagc
     1981 tgctgacttt tgccgagatt acctaccctc cgttcgtaca aaccgcatta caaaaaattc
     2041 agaaagttgc tgcacaaaaa gaagcgacga agcccgttgt tgccgccgag cagggagcgt
     2101 cgctttcaag tgacatgagt gaatttcaga acgctcttga aaccattctt ttatggtgcg
     2161 atatcccaga agacaaaaag caacagttca aaaaagattg ggatgcgttg aaaaaattca
     2221 aaaatcctct cgacggctct gacgacgata tccgtaaatt acgccgtcgt gtgggagtgc
     2281 agtattggga aatttttgag cgcggcatgc tcaaatactt acgtaaacgc accgatttac
     2341 cgcgagtggt gcgtctcttt tttgattatg ggtttatgga tgaaacacaa ctcgactcag
     2401 agcaactgac caccctcgct aacgcgagtt atggcaaagg cgagtcgtgg ctccccgttt
     2461 acaccgttac cgaatggctc gccgcaattt atgacaataa agtaagaaca tcgattagcg
     2521 agatgggcct aacctttttt gaaatgttgc gacaagatcc gtcaaaccgc gacaagaaat
     2581 ggaaacgtga aagcgatttg ccccctgaag taaattctcc agaagcgcgt gtaaaatttg
     2641 aaattaaaga aatgctacaa atgaactgcc gactttgttc ggggaacatt tcaagtcacc
     2701 tcaatatttt gacacgccac caaattacgc aagcattacc gaaagttatg atcactaaag
     2761 acactgtcgt tgatcagatt aaaaaaattc tcgccgttga ctttggagca ttccatcgtg
     2821 aaatacttta caccaacgaa cagatgaaca tccaacgcga gtttattcaa gtgcaagtga
     2881 tgcccaatat tattttagtg ccttctattg gtaccgtgat tcaattttgg caagaacgcg
     2941 aagggaatga ccgtacttca cggggacgcc tcattgcccc cattatggtc actgccgatt
     3001 tacacacaat gctactgaaa gctgtcggca cataccgttg ggaaatgtgt aaaacaaccg
     3061 caggccccga ctggaataat atttcagtgt cttctttaac ggctgactac acggactata
     3121 tacagtttta ctacaaaagt aaagaactct ccgaagaaat taaagaaaaa ttaagtgaag
     3181 actttaaacg attccgcgat gatcgtgcgc gctttgtcaa cgattacact atctatatgc
     3241 gttttgaatc ggatggttcg cagcgcatga atcgagtttc tcgtaaaatt tttacaaaac
     3301 acatcccttt tgcaaaagag attcgagaaa aattattgaa attgcctagt tacaccgatg
     3361 ttgtccaaaa atcgataaac attcgtaaaa gaaaagcaaa cgagctgacc ccgcgatata
     3421 caaaatatga acgtgaaaac aaaactctcc ccaaagaatt ggtcgaaact cgcaagtttt
     3481 acaatatgga gtattagaaa tctaaatatc cgcatgcgat tttgtgcggc ttatacactc
     3541 ctgtccttgg gagtaaacgc tgtctatgca aactccattg tcatttcagg gaaaatttat
     3601 tctgaaaaca acaacgcccc tatagagttt ggtacggttg ttatacccga ggcaaagttc
     3661 aaaagccgag tccaagcaga cggtcgatac gcaatgagcg tacctaacgc aggtacatat
     3721 acaataatta tcacttcgcc ggggcttaaa acaatccaag aaaaaattac cattacaaaa
     3781 tcgacaaccc gtgattttcg tatggagtta cccattgtaa agacacgcac ggtacgtttg
     3841 cgggcagaac gcgatataca aaaattggga cgcaacacat tatcggtaga gcaactcaaa
     3901 gaaacccgca acattcggcg acgcgttaaa cgcactggct acgctacccg gcgtcgttag
     3961 acctggggga ttctttgggc cgcttgtcat tcgaggggcg ggcgataaag caaatcgata
     4021 ttttatcgac gatattccag tgcccaaccc acagcatttt ggcggcttac aatcgattat
     4081 ctcgaacgat ttaatgcgtg aaatcgattt atacgcatct gcattcccct ctcaatttgg
     4141 aggggcgctc ggtgcaatta tcgatattca aacgatagac gaagtgaaag aactgggcgg
     4201 agtcattgac gtatcgctta tttcagcaaa ctatttagtt aagaacacat ggggcaaaaa
     4261 ttatggcggt gaaacgaccg ttacttcggc agaaaaaggg gacaacacaa cagaggaaac
     4321 ctctgcgaaa aattcatcgg caaaaggata ctggattagc tctggccgca ttgggtatct
     4381 tagcttattt gtgccgccca tctataaact gatcacgggt aaatcgctcg accgcttacc
     4441 ccaatactac gattatcaac tcaaagggaa agtattttta gacacgaaag gtcgccatgc
     4501 gctttcgctt ttagcctttg gtagttatga tactcttaaa tttgtacgta atttatctga
     4561 agaagaaaaa gcaaaacgga aagcagaagg agacgacccg tttcttgcag gttttaattt
     4621 aagtaacgat attacctcgc atagtcaagg gctttattac gactatatac cttctgctca
     4681 gatttcaaat cgattaattc tttttaatag tttcacctat agctatttct acgcgaactt
     4741 taccgataca agaaccatcg gccccatcga tttgaaaatt tatcctagta ttttgggtat
     4801 caaagataaa acgaaatggg aatgggcgtc gtttgcaaca ttacgcaccg cgcttgaata
     4861 taatctttat tatttcaaat caatgggtca aacgcaacaa cagctgaatg tttcggcagg
     4921 aggcccaccc gattatggca atttggcggg gtttgcaagt gtgcccgtcg atttttctac
     4981 tacaaatcac ttagtatcat cgtatattga aaacaaattt cagttcggcg gatggaactt
     5041 tgtgccgggt gtgcgtggag aatttctttc gcgcaatcgc tccgctatta tcgacccacg
     5101 cgcgcttatt ggctatacgt tcgatagcga tactactgtt tcggctgcag gcggacagta
     5161 ccaatcgttt tctcaaacga acactttcta tttcaaccaa gtcttcaact atcaaccgca
     5221 agtagtcgtt gccgattaca taaagccgga acgagcattc cataggacgc tcggtatcga
     5281 acaaaaaatt gtaaactata ctctaaaaat cgaagggttc ttaaacgatt ttgacagact
     5341 attggaagcg gcaaataatg gtcaagtcac agggaaatat ttttctaaca acggcgcttt
     5401 aaaagctcgt ggtgttgaag ttattatccg caaagatagc gaagagaaag aatttgatta
     5461 ttacggatgg gttagttaca cctacacaca aggagagcga ctacgctatg gcgcttgggg
     5521 taaatatgag tttgaacaac cccattcgct aaaacttgtt ggcggtataa agtggcgcgc
     5581 acatacctta ggtgcgcaat ttcaactatt ttcaggttat ccttatacga atatcaccgg
     5641 tagtacttgt actaccggtt ttgattgctc atctccaagc acggcgcgtt attcgccatt
     5701 atattcgtct aatagttatg acgggcattt taccgcgtca caccgtctcg acattcgtta
     5761 tacgcataaa accgcttata aatggggtta tttcagttgg tatatcgaag taataaacat
     5821 ctataatcag caagccgcca atcagttaaa atggaattat aacgagccct attcgtcctc
     5881 aaatcctaaa attcaaattc cagacggtat tattacaatt attccttatt ttggattaga
     5941 gtggagattt taatccgtta gaactcaata aaagcaaaga ggttgtgcgt tttgtgcccg
     6001 aacgtacttt ctttcacaag aaactcaacc ccacattcac ggatcacttc ttcaactgtg
     6061 ctgcttaccg caggtgtttg gagcacgata aaatgcgaag ggcttaacag tattgctttg
     6121 caataattcg acagttgagc ggcaattttc tgtatcaaca aagtcgattc ttgaaccgag
     6181 agcgcccttt caaaaacata ttggaaaatt aacggcgcat cgcaatcgat gagagcaagc
     6241 gattctttaa gttcgcgcac tgcaccgtta tcgagccgaa atacatcccc cttgttttca
     6301 tacgcttcaa taaaaaacat ttttatcgct ggcgcaattt gcttcaagta gcttttcgta
     6361 tttgagatca actcaaaatc gtcgagcgca ccgccacgtg tatcgtcaaa aaaaacaagc
     6421 cgcgctttca ggaagctttc gtttaaggag aaaacatgta ttcccttaaa tttttcggtg
     6481 agccctttcg gaaaacgttt tgtaaaaaag ggattttttc gtaacgccgc cgaaaattca
     6541 atatacgtgt actcatcgct cgcgatggag aaaacggaat ctttgagtaa aagataaaaa
     6601 taatctttta aattttgagg aacgtcgtgt gaaagaccga cgcgataaac ggcattcagt
     6661 ggttcgtata tcatgatggc aaacgccgac gcttgtacac cgtcgcttgc cactcgcatc
     6721 gcttcaaaga gttcgtcggc ggtgacatcg tcaaaatata attcatttaa ataatataag
     6781 tattgatcca ccgagggata acggtgtgca ttttcaaact gttcaatccg caaaagataa
     6841 tctgcggtgg tgacgctttt gacggcttct tttgtaatag gcgaagcgac ttcggtcatc
     6901 agcgttttga gttcgtcgct aaacgccttg ccgcgtattt tttcttcgcg ctcgttcagt
     6961 tgaagaattt tgggcgcgct cgctttttca gtgagagcag gattcataaa acgaaattgt
     7021 cttttctctg tatttgtctc tatggtaatt tcagtttgaa gcggcggttc tttagcgaca
     7081 aagtgtgcac gccctaatcg aattttttct tcgacggcca aaatgggatc gctaggttga
     7141 gcggataaaa aattggcgtt tatttctttt tcaacatttt catcgttgag ttttgccaat
     7201 gtctcaaatg tttgcttatg catggcaaga gcgcgtctgt ggttatctaa aattcgccct
     7261 gaaacttcgc ggcgcgtcat caccttcgcg cgatacacac gcagaatatc ggcaaacaca
     7321 acgataaaaa gtatgagggt aaagtaaatg ctccagtaag tatatattgg cggtaatggc
     7381 gacacttgaa ataggtatac gtttgcatca agccacactt gctttagcgc aacgtattcg
     7441 cggccacgta tcgagaacgc ctcacgatta ctcgttaagc gcgacaacac tccttctact
     7501 aactttttct cttcggcatt gaaggcatcg tgagaaaatg ctgccactgt ttcaaaatcg
     7561 taagaggtga caaaaagcgc agtggttgat ttttttagat cagggcggat aataaatact
     7621 gcatagccca agcgtttacc agtgaagtcg gtgatgttgt atcggcggag aagtcctgta
     7681 tctgcaagga gggtgtcggt caattgcgta cttaaaagag ttcgttcttg tagagacaaa
     7741 tcacgccccg aacgtaccaa tgttttaaaa tggtttgtta ataccaaagc tgattcaatt
     7801 aaatcgtgat tggggtttaa ctctctgacc tctttttccc tctggtcaaa tttattgcca
     7861 tgcggccgga ctcctaaata tctaaagaat tccccttgac ggtttctctc attgatttct
     7921 tgatcgatgc gctcaatatc gctgcgtaaa ctgtcaaacc gaacttcgga aagcgccgtc
     7981 gcacgattca ccaaaacaaa ccgctcggct atatacgcac ctaaggcaag agcgaaagca
     8041 agtattacaa aaatgtaaac gcgcaccatg ataattcaag ttaaccaacg gtagcgctga
     8101 taactttcaa attttttgcc gctacaaaat gcttttactt gcggcaaaca atcggggaaa
     8161 aatgccactt tgtcaattct accgagcggt atccacgcat aaccagccaa tgctccttca
     8221 cgctttacct cgatacgccc gtcaatcgcg tcgatttgaa acaccatctg aaaaacttgg
     8281 gttcgcttcg cttcgctcgg caccgattcg ccgcaaaaaa gaaacttgcc tacttgaact
     8341 tgaagattca actcttcgta ccattcgcgc ttgagtgcat cgatcgccga ttcccccaac
     8401 tcttggccgc ctcccggtaa taaatagtat tctcttcctg ctttgcggtg tttaactaaa
     8461 agaatgtcgt cgccacgcat aaaaatgcct gcgatgcgat gccgaatcgt tggtcttgcg
     8521 ctaaagaaat ttgcattact catgatagtg gcgccgcctc aaagcgaatc aataccacgt
     8581 cagcatccac ctgtgagcct gcttcaacca acacttcttg aatttttaca cgagccggcg
     8641 cgaccagagt aatttccatt ttcattgctt cttgaataat caacgctgtc ccttgctcga
     8701 ccacctgatc taccgcagca cagattttca cgatgcgccc cggaatttct gaacgaattt
     8761 cagaatggtg tactttctca ctgtctgacc cactgaaagg agtgttcgta aaacgataaa
     8821 aaaaaccgtc aaggacaagt tcgataccgt cgttagtctg gtggaaatgt gcaatgtgtt
     8881 cttttccatc ggcacggaca gtcattacac gcttttttaa atcacagtga gtaacggcaa
     8941 aaggaagcgc tttttttata ttccatgctg tacctacggt aaattgattg tttgaaacga
     9001 ttcgagacaa aacggtttgg tcgtgacgta gaaggtagcg ccgcatgaga tttgggtacc
     9061 ttgcctgagg ctttcatggt gacaacaaaa gtattataat agaagtctgt gcgccaactc
     9121 gctgcgcgag ttggcgcaca ggttcacagc cccagaaaaa tgtaagaacc agtgtcgata
     9181 tctacgagca ccggcggggc tatgaaaatt tagatgacgt taaagtattt gagctgaaag
     9241 aaaataggga ttgccgcaaa acccagtggg atgacaatcg aatacaaaag tcccaacttt
     9301 ttaatgaaag gcgtatattt tttgtggttt aatcccaacg attgaaatgc gctttggaaa
     9361 ccatggttta agtggaatga aattaaaagc atagcgagga cataaaacca aggatagaat
     9421 ccgccccaac cggtttcaaa agacgcttta acggtttcgt aaaaagccaa attgcctggc
     9481 tcataaatgc ggtgtttcag cgtgtacgag ttgatatgaa tgattaaata aactaaaata
     9541 acaagtcctg ttaaatccat atagcgagag aaaaatgatt tcacataact tttgccgcct
     9601 gaataaccga caggacgcgc tgctttgttt tggcgtgtga gaatgatgcc gtcaataata
     9661 tgaactaaaa atcccaacag taaagtgact tcaagaactc gaataattat cgacgtgctc
     9721 atgaacaagg aataagcgtt aaacgccgca ccgccgtcgt ctttgaacaa aagtaaattg
     9781 cccgccaagt gaatagggag aaagctcacc aggaaaagac ctgagagtgc catcagaaac
     9841 tttttgccga tagatgttga gaaaaatcgg ggtgaagagt tcgttgcttg agacatagaa
     9901 ccagcatcga tgaaggaaac ggcgcgcaaa gcaaaacatg aaaaggatga tgtatatctc
     9961 tcaaattcaa gttctaaata caacgatttt gcctttgttg aatttactct gcaaaaccgc
    10021 tggtttggcc gtttatattc tctaccaggg gccaaatgcg gtctaatttt tgtcatctta
    10081 cgctgtcaca gcgaattgaa attcagaagc tatttgggtc acacgacccg cgtccattgc
    10141 tcgtgttctg ggcgtcagca agtcgacgat ttcgcgcgaa cgaaaagcgc acgccgttgc
    10201 agggcgatac gatgccgaac gggcacagcg tcttgctgac atgaaggcgt tgcagaaaat
    10261 ttttgcggcg ccgtgtttta atcgcgagcg gttcggagac catcgggctg ttatgcaggt
    10321 atttgcccaa aggcacaggc tccttagtta ttgtctaacc cacggacata cctgacaaat
    10381 gctacgccgc aattgggggg tat
//
LOCUS       DAOMPD010000024        10256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2818_length_10256_cov_2.882168, whole genome shotgun sequence.
ACCESSION   DAOMPD010000024 DAOMPD010000000
VERSION     DAOMPD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_10256_cov_2.882168"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2173
                     /gene="dnaE"
                     /locus_tag="PLY93_01630"
     CDS             <1..2173
                     /gene="dnaE"
                     /locus_tag="PLY93_01630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRP68214.1"
                     /translation="AVIKDVARVLGVDYQLINALSKNLPDTPGISLQDAVDASPEIKK
                     YFEQGEKEKQLFAIAKRLEGMPRNSGKHAAGVVIAPYPLDDIVPLAKDSKTDSIVTQY
                     DMTLLPKVGLVKMDFLGLKNLTIIQHCVEEIERRRGIKLNMAEIPLDDTKTYQLLTHG
                     QTRGVFQVESTGLTKLLMQAKPRVFEDIVAIVALYRPGPLESGMTESYVKRKTGEMRV
                     EYPHASLEPILKDTYGTFVYQEQIMLASQIVGGFTLAEADTLRKAMGKKKVDVMEKMK
                     AQFVAGAKEKNHKEKWASELFDNMAEFAKYGFNKSHSAAYGFITYQTAYLKVNYTIEF
                     LKASMDADIGDTDKIISYISHAKELGIEVLPPDVNESDVYFTILDEHRLRFGLLAIKG
                     LGESPAAAIVQARAANNNRFKSLVDFVESLDSKTLNRRVLDTLTFAGALDSLGYTRAS
                     LIHSADEILKFAAAAYLDRSSGQSSLFGGDEIEVERLQIPAIQEFSDDEKLLKEKQTL
                     GLYVTAHPLDKFADASAHMNVVQIKDLDDGTSKERRLSILAVVDSLRTVTSKRGAFYI
                     LKVADRTGQIEIRIFANLYEQVKARLQENVCAIFDLRVLAIREEEITTMNYTLTGVIP
                     QEQFVERVEKSLHLYLNVKTTTALQNIIGQVKTTLRKYSGNNPVYLYWREKDGGPLQS
                     IKAHSSFCVRYDKALLKDVGHLLGAEKYALFKVGGRMEAVG"
     gene            2282..3424
                     /gene="dnaN"
                     /locus_tag="PLY93_01635"
     CDS             2282..3424
                     /gene="dnaN"
                     /locus_tag="PLY93_01635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRP68215.1"
                     /translation="MKFQTNKDTIDKAVRRIDAIIPHRDMGTMLSNLLIAIETDHVAI
                     TASDMETTARIVVPATETVVGEFIIPAKQFLEYVSSLLSEDLIVEVEKDEENEEGLSA
                     FRVNLKGTKDTSARYIMPGNSPKSFPTLNHLSNNKLFNVSSAILEEMIRKTQYAISQE
                     DNRYIYNGLSFQGEGTQLTLVGTDGRRLAAITRKLAGPVIFPPEGDIVVHAKAIREIQ
                     KLIDVSDEVLIGVEQRDLFVRVGDAQLSSRLLEGKFPDYKRVLPRELGVLLDINRNNL
                     LNALQQIRPVTEPPSNQCRMILENSRMHITGQSTGSGSRGDVTLDVAYTGEQLEFGFN
                     ITYLIDILKALNCVKVQLGLNDANKPLVVHDMDDPDFIALVMPMKI"
     gene            3431..4576
                     /gene="recF"
                     /locus_tag="PLY93_01640"
     CDS             3431..4576
                     /gene="recF"
                     /locus_tag="PLY93_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRP68216.1"
                     /translation="MIQNLQLQNFRNCQRASYTLGDGLIFITGNNGAGKTTILEALGL
                     ISFLKSFRLAHDREMLHFGASFYRIEAEYQDGNETHKIAIAYGRNTLDEKAPAQKKYT
                     FDDHPNEKAANIIGRVPSVVLSPDDMRIVAGDHTDRRRFLDLLLSMLYPAYFMALQHY
                     HRTLKLRSQTLKTKPDEALLTAIDRELALAGVALLGKRRQFIPEFGTPFGESVKKISG
                     GRDVWRIVYKGDTGKVQTIDDYLQMLKEHRANDLRLKQTTSGIHRDRIYFLQEADFSK
                     GEGFTAADFTEREIRAIGSQGQKRTAALALKAAQFTYMREKLGKTPILLIDDILNELD
                     LSRRAQFIEFLGETKIGQVFFTTTDLVGMQDFLKSLQGTTPIQQIEL"
     gene            complement(4579..5514)
                     /locus_tag="PLY93_01645"
     CDS             complement(4579..5514)
                     /locus_tag="PLY93_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRP68217.1"
                     /translation="MARFRILFVLAIALFTSCSSAQTKAAEPTADKKNYLRGLYIPVR
                     KTGGARSVPFVKSLVERGKPLGLNAVALDAQTFKGMVNKINPEVVAYLKGESIYTIAR
                     VVCFQDGIQKLPVAANHMKKLRALIAEVSQMGFDEINLDYIRFEDAGTTYPLKKKYEF
                     LDNLLAEFRTLTAQHNIKLSADVFGRIVYNEKDSIGQNIENYAKHTDVIYPMLYPSHF
                     TKDKKRMSDPGFTVSEGTEKAIERLKGTTTEIMPWVQVFVYNIQYARVKLSEYVFLQI
                     EALEKTQARGWIAWNAKAEYKELFQAMEKLSSGRQ"
     gene            complement(5525..6715)
                     /locus_tag="PLY93_01650"
     CDS             complement(5525..6715)
                     /locus_tag="PLY93_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP68218.1"
                     /translation="MSIHAHDTNLLDFNISPELEEMGNLIREICEKEVVPRRADMDEH
                     EKYPFEVFEKFKEAGLPAVMYSEEYGGAGLGLFASIMFAEIVSEYCLGVNTAISATKL
                     GALPIEHGGTEEQKKKYISAFASGEKIGAFGLTEPNAGSDVPNLSTTAVKKGDRYILN
                     GTKQWISNSGVADVYTIFALTDKSKGPRGMSCFIVEKGTKGLSYGKLEQKMGIRCSHT
                     RQVILEDCEVPEENLVGLKPNRGFIHAFQTLSSSRPAVAASAVGLATGAFKEAVKYAR
                     QREQFGKNIINFQAVQHMLADMATRIEQARLITYRAAKYAMAGHPEAAKYSAMAKYAA
                     SETAVAVANDALQLHGGYGFTKDYPIEKMYRDAKILTIYEGTSQIQKNEIGAYIIKES
                     SQLK"
     gene            complement(6776..6919)
                     /locus_tag="PLY93_01655"
     CDS             complement(6776..6919)
                     /locus_tag="PLY93_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68219.1"
                     /translation="MQNKLEQFAVGLTALHAALPEEFVQNSVQKFAKKLFQRIGGQAP
                     QSL"
     gene            7053..7994
                     /locus_tag="PLY93_01660"
     CDS             7053..7994
                     /locus_tag="PLY93_01660"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRP68220.1"
                     /translation="MRKHLLSRMGETTKRIITGVLIAAAFIFTITYQGAYMVFLYAFV
                     LAAAIIGVRELYKLAEAKIQQRISSRLAIASTVIMLTLVYVGWQAQYYVDGAPLFNAS
                     VQKVLAILKLNYAFLGGLLFVFLFFILFFQLLKNHVENSFFIIGLFVLAVFYLPFTFS
                     HLFLLYGLENGLFYIWMVAWATAMADTGGYFMGRAFGRHKVGFAVSPNKTYEGYILGG
                     VLQNILVHIFYYIARTNFNVPQYGVIEITLFGMIIYLASIVGDLAESLLKRDAGIKDS
                     SHLIPGHGGVLDLLDAMLITIPAAYYYFYIVQELHRL"
     gene            7999..9132
                     /locus_tag="PLY93_01665"
     CDS             7999..9132
                     /locus_tag="PLY93_01665"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRP68221.1"
                     /translation="MRRKFILFGATGSIGDSTLDILRQHSEDFELVGFTWHKNEAKAS
                     QIAHEFEVHHAYSTSSLDALKNIQTLLDLPHDFVLMAIVGAAGITATYEAAKRGKTIL
                     LANKESLVIAGSVINHACRKNGAKILPVDSEHSSLWRLLSPTPAPHSYPCTENAHIHC
                     AYLTASGGPLLHIQGSEFENAPKSLVLKHPTWVMGQKITVDSAGLVNKALEVIEAHHL
                     FGLPYEKIQAVIHPQSYVHAMIEHTDGTITQHVSQPDMRYPIAHAMYYPNAARMNVPR
                     KEPNSYPALEFYPIDEKKFRAYALGRMAGTKGMYFPTVFNAANEVAVTAYLKEEIRFG
                     EIANLIEYALDARYDTYDMNSVDGLMQFDNAAREITRRRVFEV"
     gene            9136..9930
                     /gene="proB"
                     /locus_tag="PLY93_01670"
     CDS             9136..9930
                     /gene="proB"
                     /locus_tag="PLY93_01670"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRP68222.1"
                     /translation="MKLKQRIESATHVVLKFGTSVLTDHIEKRNKKYFQSIAKSCLEL
                     QKQGKKVIIVSSGAVGFGRVVLKERNNLKIPQASVGEKQALASLGQSLLIDTYQIAFA
                     KNKLAAAQILVTRTDFENKKHLQKLKSTLNQLLEWSAVPIINENDAVSNEEIKVGDND
                     NLSAQIALLYPKSVLVLLTSVDAFYLDEKPVTHITRITPELRRAAGDTAEGSGGTGGM
                     ITKLQAADKILNANQLMSIAAGKDMKIIRQLMAAKPVGTWFYKDKE"
     gene            9927..>10256
                     /locus_tag="PLY93_01675"
     CDS             9927..>10256
                     /locus_tag="PLY93_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68223.1"
                     /translation="MMSEKSHIGKLTAIHGPMFSGKTEELLRRVRRAKIARKKVQLFK
                     PAIDNRYHATQVLPHFLASAGSTLTVSTKRENDGSVESVQVGEEAHVVERARDILSLV
                     DSQTDIVA"
BASE COUNT         2747 a         2531 c         2368 g         2610 t
ORIGIN      
        1 agcggtgatt aaagacgtgg cgcgggtgtt gggtgtcgac taccaactca tcaacgcgct
       61 ctccaaaaac ttacccgaca cgccaggcat ctcgttacaa gacgccgtcg acgcctcgcc
      121 ggagatcaaa aaatactttg agcaaggcga aaaagaaaaa cagctttttg ccatcgccaa
      181 gcgtcttgag ggtatgcctc gcaactcggg caagcacgcc gctggggtgg tgattgcccc
      241 ctacccgctc gacgacattg tgccgttggc gaaagattct aaaaccgatt cgattgtcac
      301 acaatacgac atgacgcttt tacctaaagt gggccttgtc aagatggatt ttctgggatt
      361 aaaaaatctc acgattattc aacactgcgt agaagaaatt gaacgccgtc gaggtatcaa
      421 actcaatatg gcggagatcc ccctcgacga cacaaaaaca taccaacttt taacgcatgg
      481 ccaaacacgc ggtgtgttcc aagtggaatc gacggggtta acaaaattac tcatgcaagc
      541 caagccgcgc gtatttgaag atattgtcgc tatcgtcgcg ctctaccgcc cgggcccttt
      601 agagtcggga atgacggaga gttatgtaaa acgtaaaacc ggtgagatgc gcgttgagta
      661 cccgcacgcc tcgctcgaac ccattttgaa agacacttac ggcacgttcg tgtaccaaga
      721 gcaaattatg ctcgcgtcac aaatcgtggg tgggtttacc ctcgccgaag ccgacacgct
      781 tagaaaagcg atgggcaaga agaaagtcga cgtgatggaa aaaatgaaag cccaatttgt
      841 cgcgggcgct aaagagaaaa atcacaaaga aaaatgggca tccgaacttt ttgacaatat
      901 ggccgaattc gctaagtatg gttttaataa atcgcactcg gccgcttatg gattcatcac
      961 ttatcaaacg gcgtatttga aagtcaatta caccatcgaa tttttgaagg cgtcgatgga
     1021 cgccgacatc ggcgataccg ataaaattat cagttacatc agtcacgcaa aagagttggg
     1081 catcgaagtg ctgccgcccg atgtcaacga gtccgacgtt tactttacta ttttggatga
     1141 acaccggctg cgctttggtc ttttagcgat taaaggtttg ggcgagtcac ccgctgcggc
     1201 tatcgtccaa gcgcgcgcgg caaacaataa ccgcttcaaa tcactcgtgg attttgtgga
     1261 atcgctcgat tccaaaactt tgaatcgccg cgtgctcgac acgctcacct tcgcaggcgc
     1321 tctcgattcg ttgggataca cccgcgcgtc actcatccat tcggcggatg aaattttgaa
     1381 attcgctgcg gcggcatacc tggatcgcag ttcggggcaa tcctcccttt ttggtggcga
     1441 tgaaatcgaa gtggagcgat tgcaaatccc cgcaatccaa gaattttccg acgatgaaaa
     1501 actgctgaaa gaaaaacaaa cgctcggcct ttacgtcacc gcgcatccgc tggataaatt
     1561 tgccgatgca tcggcgcaca tgaacgttgt gcaaattaaa gacttagacg acggaacgag
     1621 taaagagcgc cgtttatcca ttttggcggt ggtcgattct ctccgtactg tcacgtctaa
     1681 acggggcgcg ttttatattc ttaaagttgc cgatagaacc ggacaaattg aaatccgtat
     1741 tttcgcaaat ttatacgaac aggttaaagc acgcctccaa gaaaacgtct gcgctatttt
     1801 tgacttgcgc gttcttgcta ttagagaaga agaaataacc acgatgaact atacactcac
     1861 gggggtaatc ccccaagaac agtttgtcga gcgagtagag aagtcgctcc acctgtatct
     1921 taacgtgaaa acaacaaccg cactgcaaaa catcattggg caagtcaaaa cgacgctacg
     1981 gaagtactcg ggcaacaacc ctgtgtatct ctactggcgc gaaaaagacg gtggcccact
     2041 gcaatccatc aaagcgcact cgagtttttg tgtgcgctac gacaaggcac tcttgaagga
     2101 cgtgggtcat cttttgggcg ccgaaaagta cgcgcttttc aaagttggcg gacggatgga
     2161 agcggtggga tgagggtgac tccttaccca taaattagct tcgaggttta gatgagattt
     2221 tacatttttt ggtttatgtc ttgtcgcacc tttatcgtct tgtgttatac taagggtact
     2281 catgaaattt caaacaaata aagacactat cgataaagca gtccgtcgca tcgacgcaat
     2341 cattccacac cgtgacatgg gcacaatgct ctccaatttg cttatcgcta ttgaaaccga
     2401 ccacgttgct atcaccgcgt cagatatgga aaccacagcg cgtattgttg tgcccgcaac
     2461 agaaaccgtt gtcggcgagt ttattatacc tgcaaaacaa tttcttgaat atgtatcgtc
     2521 gctcctgagt gaagacctca tcgttgaagt tgaaaaagac gaggaaaacg aagaaggact
     2581 ttctgcattt cgggtgaatc tcaaaggtac taaagacacc tctgcacgtt atataatgcc
     2641 gggtaattca cccaaaagtt ttcctacatt aaatcattta tccaacaata aactttttaa
     2701 cgtttcgtcg gcaatcctcg aagagatgat tcgtaaaacg caatacgcca tttcgcaaga
     2761 agacaatcgt tatatttaca atggcttaag cttccaaggc gagggcacgc aattgacgtt
     2821 agtcggtacc gacggccgcc gtcttgctgc gattacacgc aagttagcag ggccggtaat
     2881 tttccctccc gagggcgaca tcgttgtgca cgcaaaagca atccgcgaaa ttcaaaaact
     2941 catcgacgtt tccgacgaag tattgattgg cgttgagcaa cgtgatcttt ttgtacgtgt
     3001 tggcgatgca caactgtcga gccgtttact cgaaggtaaa ttccccgact acaagcgcgt
     3061 actgccgcgc gaactcggcg tgttgttaga catcaaccgc aacaaccttc tcaatgcttt
     3121 gcagcaaatt cgtcctgtca ccgaaccacc ctcgaaccag tgccgcatga ttttagaaaa
     3181 tagtcgtatg cacatcaccg gtcagtcgac gggcagcggt agtcgtggcg atgtcactct
     3241 cgacgttgcg tacaccggcg aacaactcga gtttggtttt aatatcacgt accttatcga
     3301 catcttgaaa gcgctgaatt gcgtgaaagt acaattagga ctcaacgacg cgaataaacc
     3361 cctcgtcgtg cacgatatgg acgatcccga tttcatcgct ctagtgatgc cgatgaaaat
     3421 ttaatcgaag ttgatccaaa atcttcaact tcaaaatttt agaaactgcc agcgtgcgtc
     3481 gtatacatta ggcgatgggt taatttttat taccggcaac aacggcgcgg gcaaaactac
     3541 aatcctcgaa gcgttaggcc ttatttcatt tttgaaatcg ttccgtcttg cacacgaccg
     3601 tgagatgctc cattttggcg caagtttcta ccgtatcgaa gccgagtacc aagatggtaa
     3661 cgaaacgcat aaaattgcga ttgcgtacgg gcgcaacacg ttagacgaga aagcgcccgc
     3721 ccaaaaaaaa tacacgttcg acgaccaccc aaacgaaaaa gccgccaaca tcattggccg
     3781 cgtgccgagt gtagtccttt cccccgacga catgcgcatc gtggcgggcg accacaccga
     3841 ccgtcgccgt tttttggatt tgcttttatc gatgctctac cccgcgtact tcatggcgtt
     3901 gcaacactac caccgcacgc tgaagttacg tagtcaaacg ttgaaaacaa aaccggacga
     3961 ggcattgctt accgctatag atcgcgaact tgcgcttgct ggagtcgctc ttttaggaaa
     4021 acgccggcag tttatccccg agtttggaac cccattcggg gaaagcgtaa aaaaaatttc
     4081 gggtgggcgc gatgtgtggc gcatcgtcta caaaggtgac acgggaaaag tgcaaaccat
     4141 cgacgattat ctacagatgc tcaaggaaca ccgtgcaaac gatttgcgcc tcaaacaaac
     4201 cacgagtggc atccaccgcg atcgaattta ttttttgcaa gaagccgact tttcaaaagg
     4261 ggaaggattt accgctgccg attttaccga gcgtgaaatt cgcgcgatcg gttcgcaagg
     4321 acaaaaacgc accgctgcgc tcgcgctcaa agcggcacag tttacctata tgcgcgagaa
     4381 gttgggtaaa acaccgatac ttctcatcga cgacattttg aatgaactcg atttatcccg
     4441 acgcgcacag ttcatcgaat ttttaggtga aacaaaaatc gggcaagtgt ttttcaccac
     4501 caccgattta gtcggtatgc aggacttctt gaagtcactc caaggcacca caccgattca
     4561 acaaatagag ttgtgagatt attgtcgccc gctactcaat ttttccatcg cttggaataa
     4621 ttctttatac tccgccttgg cattccacgc aatccaccca cgcgcttgag ttttttcgag
     4681 agcttcaatt tgtaaaaaaa catactcaga caacttcacg cgtgcgtatt ggatgttata
     4741 cacaaacacc tgcacccacg gcatgatttc tgtggtagtg ccttttaaac gctcgatggc
     4801 tttttcggtg ccttcgctca ctgtgaaacc cggatcgctc atacgctttt tgtctttcgt
     4861 gaaatggctg gggtacaaca tgggataaat cacgtcggtg tgctttgcat agttctcaat
     4921 attttgaccg atactgtctt tttcgttgta aacaatgcga ccaaacacat ccgccgacaa
     4981 tttaatgttg tgctgtgcag taagcgtgcg gaactcggcc agcaaattat ctaaaaactc
     5041 gtatttcttt ttcaatggat acgtggttcc tgcgtcttca aagcggatgt aatccagatt
     5101 gatttcatca aaccccattt gagacacctc tgcgatgaga gcacgaagct ttttcatgtg
     5161 atttgctgca accggcaact tttggatacc gtcttgaaaa caaaccacac gcgcaatcgt
     5221 gtaaatgctt tcgcctttaa ggtacgccac cacctcgggg ttgattttat tcaccatacc
     5281 tttaaaagtt tgcgcatcga gtgcaacggc gtttaacccc aacggcttgc cgcgctcgac
     5341 aagcgatttg acaaatggca cgctgcgtgc accgccggtt tttcgtaccg gaatatagag
     5401 gccgcgtagg taattctttt tgtctgctgt cggttcggcg gcctttgtct gcgccgaact
     5461 acacgatgtg aatagcgcta tagcaagaac gaagagtatg cggaatctcg ccatagcgtt
     5521 acacttattt taactgcgaa gattctttga tgatgtacgc accaatttca tttttctgaa
     5581 tttggctggt accttcgtaa atcgttaaaa tttttgcgtc gcggtacatt ttttcaatcg
     5641 gatagtcttt agtaaaacca taccccccat gtaactggag cgcgtcgttt gccacagcga
     5701 ccgcagtttc agacgcggcg tactttgcca tcgccgaata ctttgccgct tcgggatggc
     5761 ctgccatcgc gtactttgcc gcacggtagg tgatgaggcg cgcttgctcg atacgcgttg
     5821 ccatgtcggc gagcatgtgt tgcaccgctt gaaagttgat gatattttta ccaaattgtt
     5881 cgcgttggcg tgcgtacttc accgcttctt taaaagcacc ggtggctaaa cccacggctg
     5941 aagccgccac cgcaggacgc gacgaactca gcgtttgaaa cgcgtggatg aagccgcggt
     6001 tcggttttaa tcccactaag ttttcttcgg ggacttcgca atcttctaaa atgacttggc
     6061 gggtatgcga gcaacggatg cccatttttt gttcaagctt accgtagctg agaccctttg
     6121 ttcctttttc gacgatgaag caagacatac cgcgtggacc tttggattta tcggtgagcg
     6181 cgaaaatagt ataaacatcg gcaacgcctg agtttgaaat ccactgctta gtgccgttca
     6241 aaatataacg gtcgcctttt ttgacggcgg tggtcgaaag gttaggcaca tccgaaccgg
     6301 cattgggttc ggttaaaccg aatgcgccga ttttctcacc cgatgcaaaa gcactaatgt
     6361 atttcttttt ttgctcttct gtcccgccgt gttcgatggg gagcgcaccg agttttgtcg
     6421 cagagatggc ggtgttaacg cctaagcagt actcactcac aatttcggca aacataatcg
     6481 atgcaaaaag tcctaagccc gcaccgccgt attcttcgga gtacatcacc gcaggcaagc
     6541 ccgcttcttt aaatttttca aaaacttcaa atggatattt ttcgtgttcg tccatgtcgg
     6601 cgcggcgggg caccacttct ttttcacaaa tttcacgaat aagatttccc atttcttcta
     6661 attcgggaga aatattaaaa tcgagaagat ttgtgtcgtg tgcatgtata ctcataacgc
     6721 gtcgaaaact atcgacgcgt caggacgtaa aggatttcag cgtttatcgc accggttaca
     6781 acgattgagg agcctgcccg ccaatgcgct gaaataattt ttttgcaaat ttttgtacgc
     6841 tattttgcac aaattcctcg ggcaaagcgg cgtgcaacgc cgtgaggccg acggcaaatt
     6901 gttccaattt gttctgcacg gggcgaagag acccgaattt gtgcaaaatt tcgttttttg
     6961 agcgaaacgc gtacaaaaat ttgtaaaaaa attgcctctt tatacacgct gcgcgagttg
     7021 gcgcacaggc cctgagatat ttgacaccct gtatgagaaa acatcttctc tcccgaatgg
     7081 gtgaaacaac gaaacgaatt atcaccggcg tcctgatcgc cgctgcgttt atttttacga
     7141 taacgtacca aggcgcgtat atggtttttc tttatgcgtt tgttcttgcg gcggcaataa
     7201 tcggtgtgcg cgagctctac aaactggcgg aagccaaaat tcaacaacgt atatcttctc
     7261 gattagcgat tgccagcacc gttataatgc tcaccctcgt ttatgtcggt tggcaagctc
     7321 aatactatgt cgacggcgca ccgctcttta acgcgtctgt acaaaaagta ttagcaatac
     7381 tcaaactcaa ttatgcattc ttaggtggat tgctttttgt ttttctcttt tttattttat
     7441 tttttcaact cctcaaaaac catgttgaaa attccttctt tattataggt cttttcgtgc
     7501 ttgcagtatt ttatctacca tttacattct cgcacttatt tttgttgtac ggtctcgaaa
     7561 acggcctttt ctatatttgg atggttgcct gggcgacagc aatggccgat acaggcggct
     7621 acttcatggg gcgtgcgttt ggccgccaca aagtgggctt tgcggtgagt cccaacaaaa
     7681 cctacgaagg ctatatttta ggcggagtgc ttcaaaacat actcgtgcat attttttatt
     7741 atattgcacg caccaacttt aacgtgcctc aatacggcgt gattgaaatc accctcttcg
     7801 gcatgatcat ctatctggca tcgattgtgg gcgacctcgc cgaatcgctc ttgaaacgcg
     7861 atgcgggcat caaagattcc agtcacttga ttcccggcca cggcggtgtg ctcgatttac
     7921 tcgacgcgat gcttatcact atccccgctg cgtattatta tttttacata gtgcaagaac
     7981 tacaccgcct ctaaaatcgt gcgtcgcaag tttatacttt ttggcgcaac ggggtccata
     8041 ggcgactcga cgctcgacat tttacgccaa cacagcgaag actttgaact ggtgggcttt
     8101 acttggcaca aaaatgaggc taaagccagt cagattgcgc acgaattcga agtgcatcat
     8161 gcgtattcca cgtcatccct tgatgcactt aaaaatatcc aaacgctact cgatttgccg
     8221 cacgactttg tcctcatggc gattgtaggc gcggcaggca tcaccgcaac gtacgaagcc
     8281 gccaagcggg gaaaaaccat tttgctcgcc aacaaagaaa gcctagtgat tgctggtagt
     8341 gttatcaacc atgcttgtag aaaaaacggt gcaaaaattt taccagtgga ttcggaacat
     8401 tccagtttgt ggcggctttt atcccccacg ccagcgccgc atagttatcc atgcaccgaa
     8461 aacgcgcaca tccactgcgc ctatttaacc gcgagcggcg gaccactctt gcatatccag
     8521 ggcagtgaat ttgaaaacgc acccaagtcg ttagtgttga agcaccctac ttgggtaatg
     8581 gggcagaaga tcaccgtcga ttctgccggc cttgtgaata aagccctcga ggtgatcgaa
     8641 gcacaccacc tctttggttt gccgtatgaa aaaatccaag cagtgatcca cccgcaaagt
     8701 tatgtacacg cgatgataga gcacaccgac ggcaccatca cccaacacgt cagtcagccc
     8761 gacatgcgtt atcccattgc gcacgcgatg tactacccta atgccgcaag gatgaatgtt
     8821 ccacgcaaag aaccgaatag ttatcccgca ctggagtttt atcccatcga tgaaaaaaaa
     8881 tttcgtgcgt acgcactcgg gcgaatggct ggcacaaaag ggatgtactt tcccactgtc
     8941 tttaacgcgg cgaatgaagt ggcggtcacc gcgtacctta aagaagaaat ccgttttggt
     9001 gaaatcgcta accttattga atatgcactc gatgcgcgtt acgatacgta cgatatgaac
     9061 tccgtcgacg gcttgatgca atttgacaat gcggcgcgag agataacccg ccgtcgagta
     9121 tttgaagttt aacgtatgaa gctaaagcaa cgcatcgaga gtgcaacgca tgttgtctta
     9181 aaatttggta cgagtgtact taccgatcat attgaaaaac gcaacaaaaa atattttcag
     9241 tcgattgcaa aatcctgctt ggaactacaa aagcaaggca agaaagtaat catcgtttcg
     9301 agtggcgcgg tgggctttgg ccgtgtggtg ctcaaagagc gcaacaattt gaaaatccca
     9361 caagcgtcgg tgggtgaaaa gcaagcactg gcgtcattgg gacaaagcct cctcatcgat
     9421 acctatcaaa ttgcgtttgc aaaaaataaa ctggcggcgg cacaaatttt agtaacgcgc
     9481 accgattttg aaaataaaaa gcatttacaa aaattgaaat cgacactcaa ccaattactt
     9541 gaatggagcg ctgtgcccat tatcaacgaa aacgacgcgg tatcgaacga agaaatcaaa
     9601 gtgggcgata acgacaacct gtcggcacag attgcgcttt tataccccaa gtcagtgctg
     9661 gtgctcttga cctcggtgga tgctttttat ttagacgaaa agcccgtgac ccacattact
     9721 cgtatcacgc ccgagctgcg ccgtgctgcg ggcgacactg ccgaagggag cggtggcacc
     9781 ggtggtatga ttaccaaact ccaggccgcc gataaaatac tcaacgccaa tcaactcatg
     9841 agtatcgccg ccggtaaaga catgaaaatt atacggcaac tcatggcggc aaagccagtg
     9901 ggtacatggt tctacaaaga taaagaatga tgagtgagaa atcccacatt ggaaaattga
     9961 ccgcgatcca cggccccatg ttctccggta aaaccgaaga attattgcgg cgtgtgcgac
    10021 gggcaaaaat tgcacgcaag aaagtacaac tcttcaaacc tgccatcgac aaccgttacc
    10081 acgcgaccca agtgctgccg cattttttag caagtgcagg aagtacgctc actgtctcta
    10141 caaaaagaga aaacgatggt tcagtagaat cggtgcaagt gggcgaagaa gcgcacgttg
    10201 tggaacgcgc gcgcgatatt ttgtcgctcg tcgattctca gaccgacatc gtcgcc
//
LOCUS       DAOMPD010000025        10170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2843_length_10170_cov_2.603065, whole genome shotgun sequence.
ACCESSION   DAOMPD010000025 DAOMPD010000000
VERSION     DAOMPD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_10170_cov_2.603065"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLY93_01680"
     CDS             complement(<1..644)
                     /locus_tag="PLY93_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRP68224.1"
                     /translation="MREVYIIGADETEHGSFKDKNFRHLIAEAGNGAIQDAGIDRKEI
                     QAVYMGNYNGNGLNNQNTMAALAATSLGMPYAATLRFEAACASGGVAFRNAMIAVGAG
                     VYDTVMVLGCEKMNMPPEEVPYEDQMKAALSGVDYLYEAGSGMNGSTMFAMFAHRHMH
                     EFGTTRKQLAEVATKNKYHGSLNPYAYKQREVPLEKILKSPIIASLFSAQEVSL"
     gene            863..>1219
                     /locus_tag="PLY93_01685"
     CDS             863..>1219
                     /locus_tag="PLY93_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRP68225.1"
                     /translation="MKAPLHTAHPWHGVSIGSEAPATVTAFIEIVPSDTIKYEIDKPS
                     GIMKVDRPQKFSSLCPTLYGFVPQTYCGKNIATLAADPRVERGDGDPLDICVFTERVI
                     VANGFLVRASPIGGFRL"
     assembly_gap    1220..1229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1230..1499
                     /locus_tag="PLY93_01690"
     CDS             <1230..1499
                     /locus_tag="PLY93_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRP68226.1"
                     /translation="GVMRMIDQGEADDKIIAVMVDDPLYANVSTLNDMPSALVDRLRH
                     YFLTYKEIASEKAPIIEIGAVYERDTAYKVIQAAIEDYDTDFRGQ"
     gene            2124..2876
                     /locus_tag="PLY93_01695"
     CDS             2124..2876
                     /locus_tag="PLY93_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP68227.1"
                     /translation="MPAQIISLTNIRKEYPLGETTVSALKGVDFSVDEGDLLSVVGPS
                     GSGKTTLLNIIGCIDKPTSGTALIDGEDITTLKDDALTDLRLYTVGFIFQSFNLIPVL
                     NVLENVEFPLMLMKKNAFSKAEIRKRAEELIEKVGLLEQIEQRPAELSGGQRQRVAIA
                     RALVTNPKIVLADEPTANLDSETGFKILELMRQLNEERRTTFVFSTHDPDVMQYARRI
                     VKIKDGLIAHDAVSHSAKAKKKTASLKKRRTL"
     gene            2873..4399
                     /locus_tag="PLY93_01700"
     CDS             2873..4399
                     /locus_tag="PLY93_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRP68228.1"
                     /translation="MKIAFIIALRNLLRHRGKSLIVGVILFLGALIMTLGNAVVSGMN
                     RGLEENIVERFTGHIVVISTEQENPNILFIPMGKDVALIRDFSKVKTVLDKEKYIQQY
                     LPAARGFSLVLAEDGDIDLAMLLGVDFPKYNAMFHNNVQILEGKLLSEGERGMLVSSD
                     RRERYFNDQNIWLKPMGFPLDEKNFTDEARKYRDKMIIKEGIVLMGASNDSTAFDVYV
                     PIRGIHRYAFLDDVWKFFNIVDIDTFREAFQWVTNADAEVKLNKETTALLNDNQNLDD
                     LFKEETLVDASQVKNKIQTSLKNLKAKKSIPLKKTDTGAYNVVFVKLKHGDDIPTRIA
                     SLNAALTNAGAPARAVTWKDAIGQIGDMAGIIRGALMGFVFLIFFVAVIIIMNTLSMA
                     ALERTAELGMMRAVGAPKSFVTQMFFFETLALAFLFGGLGIGIGIAGVPILNALHITT
                     EHTILQLLFGGKTFAPYLSLSDIFTGVFELGMVTLVASLYPIFLARRMTPLDAVSRDT
                     "
     gene            4396..6162
                     /locus_tag="PLY93_01705"
     CDS             4396..6162
                     /locus_tag="PLY93_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRP68229.1"
                     /translation="MSRLKLLIEFALRNLMRQKRRNALLGVAIAFGTMILIVANSFSR
                     GITDTLLNKVVVRLTGHIELAISERSRPQNLIIRDKAKYLDLLKKNIPEIREVRENIG
                     ILARAIGNGKGENAMIYPMDLGDGSDFKNHIDTDYLLSRTESGNIEDILSTRYENPVA
                     IFADKAKALRVNLLDTLKVRLKTITGQEQSARFTVVMLLKSTSFFESNAILVPLKNLK
                     TLRGLRANETGALQVVLNKITSPEAVKKLADKIHTLFHPGIAALHGQLVAHSKETRIT
                     IAGLTQEYKKNPLVTQSISLTEGEYPRDNATDTMMLSAQMAKFLDVSVGDSVTLRFKT
                     IHQTNDAERNFIVAGIFSKPPFTDVGAFLPEAAFYKTWLEDFPEKPLLYEKSIPAEWK
                     NLLAPEFRLLPRTANLDELVKKQQGLRKTNFYGPAIDVRTMYETASHALELEKVLNLI
                     TFIAVLVIFFIILIGVVNTLRMTIRERTREIGTMRAIGVYAKDVRNIFILETLLLTLI
                     ASVVGLTLALLAMLSLSWITIDTQSALGILLDKKHLYFVPHALDILRNIFVILVIAAV
                     TAYFPSRRAAQLDPAAALRSYE"
     gene            6390..7145
                     /locus_tag="PLY93_01710"
     CDS             6390..7145
                     /locus_tag="PLY93_01710"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRP68230.1"
                     /translation="MKKIILTLCFTPLMILSAVDVNHILREIDNRMSQVSDFSAKVAL
                     TQQKAGQGVKNFETLFFRRDADDEFLMVTTAPQSEKGNGYLRTGSNFWMYRQNTRTFQ
                     HINRDESINGTDTKAGDFERKKLTTLYKPFADAHGREDLIETTLGNKKVWKFTVVAKV
                     RDVTYPKQIYWVEKDTFLQLKVESYSLSGTLMQTAYFTKYTQIQGKYILLKGIFVDEF
                     EKGNKTILDISGVSLKKLDRKIFTKAYLENLSK"
     gene            7146..8495
                     /locus_tag="PLY93_01715"
     CDS             7146..8495
                     /locus_tag="PLY93_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68231.1"
                     /translation="MRSLFFVIGFLSLLQPLFPVPKEIPLDENELFSEETVTPVEKLK
                     DETIQDVAEKEGVAFSGSINTRNAYAMNRNWLLGKNASTAQNQLNNRLQNNFTLEARA
                     RHGTRAIVNFVNNIYAQSKSELKTMTDTATGQNKTYSESTNADYRFTEAFVDTNIARA
                     VYFRVGKQVLVWGRGFFWNPSDVINAELRSATDAFQFREGTYGLRMHIPVGTFFNFYS
                     FTGVGNSNRAEDVLQALKAEFALKYGEVAFGTVVGKNKVPTLIAEGSARIATIDVRVE
                     TTFAKGDNRPRIDSQTFNTLNPTTYLLRDTPVFKGVLSVGRSFELFDVADRLGINLEY
                     FYNGAGYKENIFDKNAPLVNYFVQNGIYRPNYYGMHYLGFFVTVAKFFVNDMTLALNG
                     FTDLSDGTLVASEMLSYAPLFNFTLTWQNNIFTGNPRGEYTTSGMGWYTELSARVTF"
     gene            complement(8527..9174)
                     /locus_tag="PLY93_01720"
     CDS             complement(8527..9174)
                     /locus_tag="PLY93_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HRP68232.1"
                     /translation="MKNTQIFLPIWVMLLFLGCNNYSLLEKLENPAGITTTRTRCGGG
                     PCRIFLETNLGAGFTGNFVDVFGADAKCQASFDKPSSGIYKALISDDPVRRVCTTANC
                     SGATGMEQSKDWVLYPNQQYMRADGVTPIGMTNAAGIFDATLMNAIEPTKTPSAVWTG
                     FALSSWLVDVATCNKWTQTSGYYGRVGDATLPENVAFSNTQKSCTELASLYCVEQ"
     gene            9303..>9736
                     /locus_tag="PLY93_01725"
     CDS             9303..>9736
                     /locus_tag="PLY93_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP68233.1"
                     /translation="MVDKLRAEFPFFEANPGIVYLDSGATALKPQRVVDALMQYTARE
                     SVNIHRGVYHLSQRATDTVEEVRKKAANFVSHNANHVAVFARGTTEGLNLAAHILTSP
                     RSGLKDFFKSYASPKEPAILLSESEHHANIVPWQIYAVERGY"
     assembly_gap    9737..9746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9747..>10170
                     /locus_tag="PLY93_01730"
     CDS             <9747..>10170
                     /locus_tag="PLY93_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP68234.1"
                     /translation="PVDSDGNYNAPPHFLDDLSSRYSIRVVSLSLQSNVTGILHDTRA
                     YRDFAKATGAAFVVDGAQAVVHAPHAVGELNADFVAYSAHKLFGPTGIGALVADKSVL
                     DACDVYQGGGGMINLVEHDKSTYLDAPGKYEAGTQAIGE"
BASE COUNT         2721 a         2546 c         2336 g         2547 t
ORIGIN      
        1 attaaagaaa cttcttgcgc gctgaaaagc gaggcgatga tcggcgattt caaaattttc
       61 tcgagcggca cttcgcgttg cttgtacgca tacggattca aactaccgtg gtatttgttt
      121 ttagtcgcaa cttcggcgag ttgtttgcgc gtcgtgccaa actcgtgcat gtgccggtgt
      181 gcgaacatcg cgaacatcgt cgaaccgttc atgcccgacc ccgcttcgta gaggtagtcg
      241 acgcccgaaa gcgcggcttt catttgatct tcgtacggca cttcttcggg cggcatgttc
      301 attttttcgc agcctaaaac catcacggta tcgtagacgc ccgcgccgac ggcgatcatc
      361 gcgttacgaa acgcgactcc acccgaagcg catgccgcct caaagcgcag cgtggctgcg
      421 tacggcatac ctaaagatgt cgcggcaagc gcggccatcg tgttttggtt gttaagcccg
      481 ttgccgttat agttacccat gtaaacggct tgaatttctt tgcggtcgat acccgcatct
      541 tggattgcgc cgttgcccgc ttcggcgatt aaatggcgaa aattcttatc tttgaaactg
      601 ccgtgctcgg tctcgtcggc gccgataatg tagacttctc tcatatatcc tcctcgagtt
      661 tacgccagtg gcgccgtagc tatggaaatc cacagccagc ggacgccaat attgtagcat
      721 acgcgaaaga caaatccgaa aacttgaacg acgcgtcgac ccttttcgac gggttatcga
      781 cttcaatagc tcgcgagcct gtgcgccgac tcgcgcagcg tgaatcctct ttccattcta
      841 tcaaccagac ataagatgcc gtatgaaagc tcccttgcac accgcgcatc cttggcatgg
      901 cgtttcgatt ggcagcgaag cccccgcaac ggttacagca ttcattgaaa tcgttccatc
      961 cgatacaatc aaatacgaaa tcgacaaacc ttccggcatt atgaaagtcg accgtccgca
     1021 gaagttttca tcgctctgcc caacgctcta cggcttcgtg ccacaaacct actgcggaaa
     1081 aaatatcgct actctcgctg ccgacccgcg cgtcgaacgc ggcgatggcg atccactcga
     1141 catctgtgtt ttcacagaac gtgtgattgt cgccaatggt tttcttgtgc gcgcaagccc
     1201 catcggcggc tttcgtctcn nnnnnnnnng gagttatgcg aatgattgat caaggtgaag
     1261 ccgacgataa aattattgcg gttatggtgg acgaccccct ctatgccaac gtcagcacct
     1321 tgaacgatat gccgtctgcg ctcgtcgaca ggttacgcca ttatttcctg acatacaaag
     1381 aaattgcatc agaaaaagcg ccgattattg aaattggcgc agtctatgaa cgcgacactg
     1441 catataaagt aatccaagct gcaattgagg attacgatac cgatttccgc ggtcagtaat
     1501 tgaagttcca gcagaactaa cgctcgcaaa aacgatgcca acgggagctt tattatgcta
     1561 cgttaggaag ttctacacac ccgataaaat ttaaagcgac gcgcctttta ggcgagcgtg
     1621 tcgcgggagc tcctaaccgg tgttctcgcc tcactctcca ccgagggtaa aaaattcaag
     1681 ttgccatact gcgcccataa agttttaata acaaatagat atacccgata gagacgactt
     1741 ggaacaccac ttaggggctc catttcgttt ggatgacagt tttgcttcgc agtgggggat
     1801 tgaacgtatc ctcgaaacgg tgcgtcgcat gtctacttgg ccaaaacatg tcgcatagct
     1861 cagtcgatac atggccgatt tttttgcaga ggcattccat gccatgcaaa ttccattcac
     1921 ccgcactggt atcttcgcaa aattatctta tacgcctcgt tgctgcggag ttttgtaaac
     1981 aacaagcaat taagcccgaa aaccaaaatg atgcatccgc gtaccgcgaa tcaaaaggtt
     2041 ggcgcacagg ctcctaacta taactattcc taaaaattgt taataccatc agattttagt
     2101 attttcactt aactggataa aaaatgcctg cacaaattat ttctctcaca aacatccgca
     2161 aagaataccc actcggcgaa accacggtga gtgcgctcaa aggtgtcgat ttttctgtcg
     2221 atgagggcga tctcttgtcg gtggtgggac cgtcaggaag cggtaagacg acgctcctca
     2281 acatcattgg ctgtatcgac aagcccactt cgggcacggc gctcatcgac ggcgaagaca
     2341 tcaccacact caaagacgat gcactcaccg atttacgcct ctatacagtg ggatttattt
     2401 ttcaatcgtt caatttaatt ccggttttga atgttcttga gaacgttgag tttccactca
     2461 tgcttatgaa aaaaaatgcg ttctctaaag cagaaattcg taaacgcgcc gaagaactca
     2521 tcgagaaagt ggggcttctt gagcaaatcg agcaacgccc cgccgagctt tcgggagggc
     2581 aaagacagcg tgtcgcgatt gcgcgtgcgc tggtcaccaa cccaaaaatt gtgttggccg
     2641 acgaacccac cgcaaatctc gattcggaaa cgggcttcaa aattcttgag ctcatgcggc
     2701 aactcaacga agagcggcgc acgacattcg tgttttcgac gcacgatccc gacgtgatgc
     2761 agtatgcacg gcgtatcgtc aaaatcaaag atggacttat cgcgcacgat gcagtatcgc
     2821 attctgctaa agcgaagaag aaaacggcat ctctcaaaaa aagacgcact ctatgaaaat
     2881 cgcgtttatt attgcgctgc gtaatttact tcgacatcgg gggaaaagcc ttatcgtcgg
     2941 tgtgatactt tttttgggtg cgctcattat gaccctgggg aatgccgtcg tctcggggat
     3001 gaaccgtggt cttgaagaaa atattgtcga acgcttcacc ggtcatattg tggttatttc
     3061 caccgaacaa gaaaatccta atatcctttt tattcccatg ggaaaggacg ttgcgctcat
     3121 ccgcgatttt tctaaagtaa aaacagtcct cgataaagaa aagtatatcc aacaatactt
     3181 acccgctgcg cgtggattta gtcttgtgct ggccgaagac ggcgacatcg atttagcgat
     3241 gcttttgggc gtggacttcc caaagtacaa cgcgatgttc cacaacaacg tgcaaatcct
     3301 cgaaggcaaa cttttaagcg aaggcgaacg cggcatgctg gtgagttctg accgccgcga
     3361 gcggtatttt aacgaccaaa atatttggct aaagccaatg ggatttcccc tcgacgaaaa
     3421 aaatttcacc gatgaagcga gaaaataccg cgataagatg atcataaaag aaggaattgt
     3481 gctcatggga gcgtcgaacg attccaccgc gttcgatgtt tatgtgccga tacgcgggat
     3541 ccaccgctac gcattcttgg acgatgtgtg gaaatttttt aacatcgtcg acatcgacac
     3601 attccgcgag gcattccagt gggtgactaa cgccgacgca gaggtaaagc tcaataaaga
     3661 aacgactgcg ctcttaaacg acaaccaaaa tttagacgac ctctttaaag aagaaactct
     3721 ggtcgatgca tcgcaagtga aaaataaaat tcaaacgtcg ctcaaaaact taaaagcaaa
     3781 aaagagcatc ccgcttaaaa aaaccgacac tggcgcgtac aacgtcgttt ttgtgaaact
     3841 aaaacacggc gacgatatcc cgacgcgcat cgcatcgctc aacgccgcgc tcaccaacgc
     3901 gggagcgccg gcacgtgcgg ttacatggaa agatgccatc ggccaaatcg gcgacatggc
     3961 gggtatcatc cgtggtgcgc tcatgggttt tgttttccta atttttttcg tcgcagtgat
     4021 tatcattatg aacacactgt cgatggcggc gttggagcgc accgccgagt tgggcatgat
     4081 gcgcgcggtg ggtgcaccca aatcttttgt gacacaaatg tttttttttg agacgctggc
     4141 gctcgcattc ctttttggcg gtttaggtat tggcatcggc atcgctggtg ttccaatttt
     4201 gaacgcgctc catatcacca ccgagcacac aattttacag ctactctttg gcggtaaaac
     4261 tttcgcacca tatctttcgc taagcgatat ttttaccggc gtgtttgaac tgggaatggt
     4321 gactctcgtc gcatcgcttt accccatttt cttggcacgc cgcatgacac cgctggatgc
     4381 cgtatcgagg gacacatgag ccgcttaaaa ctccttatag agtttgctct ccgcaattta
     4441 atgcgtcaaa aaagacggaa cgctctttta ggcgttgcga ttgcattcgg tacgatgatc
     4501 ctcatcgtcg caaactcctt ttcacgcggc atcaccgaca ctctcttaaa caaagtggtt
     4561 gtgcgtctca caggccacat cgaactcgcc atcagcgagc gttcgcgccc acaaaattta
     4621 atcatccgcg acaaggccaa gtacctcgac ttgctgaaaa agaacatccc tgaaattcgc
     4681 gaagtgcgcg agaacatcgg catcttagcg agagcaatcg gaaacggtaa aggcgagaac
     4741 gcgatgattt accccatgga cttgggcgac ggcagtgatt tcaaaaacca tatcgatacc
     4801 gattatcttt taagccgcac tgaatcgggc aacattgaag acatactttc cacccgctac
     4861 gaaaacccgg tggctatttt tgcagacaaa gccaaagcgc tccgggttaa tcttttagat
     4921 acgcttaaag tacgattaaa gacgatcacc ggtcaagagc aaagtgcgcg ctttaccgtt
     4981 gtgatgcttc tcaaatccac cagttttttt gaatcgaatg caattcttgt accgctaaag
     5041 aatctaaaga cgctgcgtgg attacgcgct aacgaaaccg gagcgctcca agtggtgttg
     5101 aataaaatca cctcacccga agcggtcaag aaattggccg ataaaatcca tacgcttttc
     5161 cacccaggga ttgccgctct ccatggccaa ttagtcgcgc actctaaaga aacacgcatc
     5221 accattgcgg gtttaaccca agaatataaa aagaatccac tcgtgactca aagtatttct
     5281 ttaaccgaag gcgaataccc gcgcgacaat gcaaccgaca cgatgatgct ctctgcacaa
     5341 atggcaaaat ttctggatgt atctgtgggc gacagcgtaa cgctccgctt taagacgata
     5401 caccagacca acgacgccga aagaaatttt atcgtcgctg gtattttctc taaaccaccc
     5461 tttaccgacg tgggcgcatt cttacccgag gctgcgtttt acaaaacgtg gttggaagac
     5521 tttcccgaaa agccactcct ttacgaaaag tccattcccg ccgaatggaa aaatttactt
     5581 gcacccgaat ttcgattact cccacgcacg gcaaatttag acgagttggt taaaaaacaa
     5641 caaggcctac gaaaaacaaa tttttacggc cccgccatcg acgtacgcac gatgtacgaa
     5701 acagccagcc atgcgttgga acttgaaaag gtattaaacc tcattacgtt tatcgcagtg
     5761 ctcgtaattt tcttcatcat cctcatcggc gtggtgaaca ctctgcgtat gaccatccgc
     5821 gagcgcacgc gcgaaattgg cacaatgcgc gccattggtg tttatgcaaa ggacgtgcgc
     5881 aatattttca ttctggaaac attgctattg actctcattg catcggtagt gggactcact
     5941 ctcgcgcttc tcgctatgct ctctctttcg tggatcacaa tcgacaccca aagcgcgttg
     6001 ggtatcttgc tcgacaaaaa gcatttatat tttgtgcccc acgctttaga tatcttgcgt
     6061 aacatttttg ttattttagt cattgccgca gtgaccgcgt acttcccttc gcggcgggcg
     6121 gcgcagctcg accctgcggc ggcgctacgt agttatgagt aagtttgttt ggaaaggtag
     6181 gtagagttgg atacccaaaa ggtggcgaca tattgggagt ttttgtaccc gactcactta
     6241 aaattttatt cgaacccgtt caaacgcgct gtttttttaa aataattcct ttgaatccaa
     6301 ttcaagtcac ggcagttatt gacgatgcgg ctgtacgcca ttttagtgta cgtatcgttt
     6361 aacactttaa aaagaccaaa gataatctta tgaaaaaaat aattcttaca ctctgtttta
     6421 ctccgcttat gattttaagc gcggtggatg taaatcatat ccttcgagaa atcgacaacc
     6481 gcatgtctca agtgagcgat ttttcggcga aggttgcgct aacgcaacaa aaagcgggac
     6541 aaggggtaaa aaattttgaa acgctttttt tccgccgcga cgccgacgat gaatttttaa
     6601 tggtaactac cgcgccacaa tcggaaaagg ggaatggtta tctacgcacc ggcagtaatt
     6661 tctggatgta tcgacaaaac acacgaacat tccaacacat caaccgcgat gagagcatca
     6721 acggtacaga caccaaagcg ggcgactttg aaagaaaaaa actcaccacg ctttacaagc
     6781 catttgccga cgcgcacggc agggaagacc ttatcgaaac gacgctgggg aataaaaaag
     6841 tgtggaagtt taccgtcgtc gccaaagtgc gcgatgtgac ataccccaaa caaatttatt
     6901 gggttgaaaa agatactttc ttgcaactca aagtggaatc gtattcgctc tcaggaacgc
     6961 tgatgcaaac cgcgtatttt accaaataca cccaaatcca aggaaagtat attttgctca
     7021 aaggcatctt cgtagacgag tttgaaaagg gtaacaagac tattctcgat atttctgggg
     7081 tttcgcttaa aaaactcgac cgtaaaattt tcacaaaagc gtatctcgaa aatttaagta
     7141 aataaatgcg ttcacttttt tttgtaatcg gatttctttc tttactacaa cctctttttc
     7201 cggttccaaa agaaattcct ctcgatgaaa atgaactttt ctcagaagag acagtgactc
     7261 ccgttgaaaa attaaaagac gaaacgattc aagacgttgc tgaaaaagaa ggagtcgctt
     7321 tttcgggttc gatcaacacc cgcaacgcgt acgcgatgaa tcgtaactgg cttttgggta
     7381 agaatgcatc gacagcgcag aatcaactga ataaccgact gcaaaataat ttcactctcg
     7441 aagcgcgcgc acggcacggt acacgggcga ttgtcaattt tgtgaacaac atttatgcgc
     7501 agagtaaatc cgaattgaag acgatgaccg acactgcaac cggccaaaac aaaacgtata
     7561 gcgagagcac caacgcggac taccgattca ccgaagcgtt cgtcgacacc aacatcgcac
     7621 gcgcggtgta ctttcgtgtg ggaaaacaag tactagtgtg ggggcgcggt tttttttgga
     7681 acccctcgga tgttatcaac gctgagttga ggagcgccac cgacgctttc cagttccggg
     7741 aaggcacgta tgggttgcgc atgcacattc cggtggggac gttttttaat ttttattcgt
     7801 tcaccggcgt cggtaactca aaccgcgcgg aagatgtgtt gcaagctctt aaagcagagt
     7861 tcgcgcttaa atacggcgag gttgcgttcg gcacggttgt gggtaaaaat aaagtgccca
     7921 cgttgatcgc cgagggtagc gcacgcattg cgaccatcga cgtgcgcgtc gagaccacgt
     7981 ttgcaaaagg cgacaaccga ccgcgcatcg acagtcaaac ttttaacacc ctcaatccca
     8041 caacgtacct cttacgcgac acgccggttt tcaaaggcgt gttgagcgtg gggcgtagct
     8101 ttgagctttt cgatgtcgcc gaccgtttgg ggataaatct cgaatatttt tacaatggcg
     8161 cgggttataa agagaatata ttcgacaaga acgctccgct tgtgaattat tttgtgcaaa
     8221 acggcattta ccgtcccaat tattatggca tgcattactt aggatttttt gtgacagtgg
     8281 caaaattttt tgtaaacgat atgacgctgg ccctcaatgg atttacagat ttaagcgacg
     8341 gcaccctcgt cgcttcggaa atgctttcgt acgcgccact tttcaatttc accctcactt
     8401 ggcagaacaa tatttttacc ggcaaccctc gcggcgaata caccacaagc ggcatgggtt
     8461 ggtacaccga gctttcggcg cgggtaactt tttagtaaag ttaattttgc cggtcgttaa
     8521 acaaacctac tgctccacac aataaaggct tgccagttcc gtacaacttt tctgtgtatt
     8581 tgaaaaagca acattctccg gtagagtcgc atcgcctact cgaccataat acccgctcgt
     8641 ctgcgtccat ttattgcagg ttgcaacatc gacgagccat gaacttaaag caaaccctgt
     8701 ccatacagca ctgggggttt tggtaggctc aatggcgttc attaaagtag cgtcgaatat
     8761 acccgccgca tttgtcatgc caatgggagt tacaccatcg gcacgcatat actgttgatt
     8821 tgggtacaag acccaatctt tgctttgctc catgccagtc gctcccgaac aatttgcagt
     8881 ggtacagaca cggcgtaccg gatcgtcgct aatgagcgcc ttataaatac ctgacgaagg
     8941 tttgtcgaag gatgcctggc acttagcgtc tgccccaaac acatccacga aatttcctgt
     9001 aaagcctgca cctaagtttg tttctaagaa aatacggcac ggcccgccgc cgcagcgcgt
     9061 cctcgttgtc gttattcccg caggattctc gagtttctcc agaaggctat agttattaca
     9121 gcccaaaaac aagagcatga cccaaattgg taagaagatt tgtgtatttt tcataagata
     9181 taagacgaat aaaaatatta atttacgaca caatttattg gtaacgcttg cgtaggttcc
     9241 tgtgcgtcat tgccttattg ttgttgacat tattagaata tttgtgtata ataatagagt
     9301 ctatggtcga taaattacga gcagagtttc ctttttttga ggcaaatccg ggtattgtgt
     9361 atctggattc gggagcaaca gcgttgaaac cacagcgggt ggtggacgcg ctcatgcaat
     9421 acaccgcacg cgaatcggtg aatatccacc gtggcgtgta ccacctttcg caacgcgcta
     9481 ccgacaccgt cgaagaagtg cgcaaaaaag cagcaaactt tgtgtctcat aacgcaaatc
     9541 acgtcgcggt gtttgcgcgc ggcaccaccg agggcttaaa tttagccgcg catatcttga
     9601 cttcaccgcg ttcaggcctc aaggattttt ttaaaagtta tgcctcaccc aaagaaccgg
     9661 caatcttgtt gagtgaaagc gaacaccacg cgaacatcgt accgtggcaa atttacgccg
     9721 tcgagcgtgg ctataannnn nnnnnncccg tcgatagcga tggcaactac aacgcaccgc
     9781 cacatttttt ggacgattta tctagtcgtt actccatccg cgtggtgtcg ctcagtttgc
     9841 aatcgaatgt cactggaatt ctccacgata cgcgcgccta ccgcgatttt gcaaaggcca
     9901 cgggagcggc ctttgtcgtc gatggtgcgc aagcggtggt gcacgcgccc cacgctgtcg
     9961 gcgaactcaa cgccgacttt gttgcgtact cggcgcacaa actttttggt cccaccggca
    10021 ttggcgccct tgtggccgac aaaagtgtcc tcgacgcttg cgatgtgtac caaggcggtg
    10081 gcggcatgat caacctcgtc gagcacgaca aatcgactta tctcgatgcg cctggaaaat
    10141 acgaagcggg cacacaagcc atcggtgaaa
//
LOCUS       DAOMPD010000026        10061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2888_length_10061_cov_2.702279, whole genome shotgun sequence.
ACCESSION   DAOMPD010000026 DAOMPD010000000
VERSION     DAOMPD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2888_length_10061_cov_2.702279"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLY93_01735"
     CDS             <1..464
                     /locus_tag="PLY93_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRP68235.1"
                     /translation="HLCYLISRGLKTFPLRMREHNRSTLAIATWLEKHPKIEKVWYPL
                     LPSHPDYALAQKYLSGGGGVVSFCIKAEDVSQKTRMTLANTFLNRLKIPTIAASLGGV
                     ESLIHHVAIMSYADLTPEKREEIGIPDNLIRFAVGLEDTDDLIADLTQALG"
     gene            complement(473..1810)
                     /locus_tag="PLY93_01740"
     CDS             complement(473..1810)
                     /locus_tag="PLY93_01740"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRP68236.1"
                     /translation="MIVAVGVIGVVALLFGLPLFIGLGTLGTLYQTTNAIELGSTTVG
                     DLTRQLTVVFSDPLSKLTGDQSALFLTIPYFTLTGYLIARSQFAARVVNLYLIVIRRV
                     GKVGTVGLGLIAILIAALFTPLTGASGVTIVAIGGILFPILTGAGYSEKRALGLITAS
                     GSIGLLFSPSLPVILFGIMSNNKADINELYRSGVLPGIVLLLSLSLVVFFYSFSKKMS
                     VANTLHTSTAHISFTRSDAVKLTLEIAAIPLLYLLMHSRYMIITELGVFFLLYYFLLE
                     VVIFREIRFQKLMAVFEEAIAMVGSIILIIFFAMILTNALLYDGVPAKLFGFISQYIH
                     SPLTFLIVLNIFLLIVGSLMDIFSAIIVIVPLIIPIAESYQIPMLHLGILFLTNLEIG
                     YLTPPVGLNLFLSSLRFQKPMNEIYKSIIPYLLVLLLAQIAITYIPWLSLWWK"
     gene            complement(1807..2142)
                     /locus_tag="PLY93_01745"
     CDS             complement(1807..2142)
                     /locus_tag="PLY93_01745"
                     /inference="COORDINATES: protein motif:HMM:NF040082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68237.1"
                     /translation="MSEKKEITLRVVLDENAIPESITWDAPGASKGEEKAEAFMLSLF
                     SGEEKTTLGIDLWTKDMLVGDMNIFFYQVLKKMADVLDRATQDKEAAQMIKTFSREFG
                     KKVKLLEEP"
     gene            complement(2146..2745)
                     /locus_tag="PLY93_01750"
     CDS             complement(2146..2745)
                     /locus_tag="PLY93_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68238.1"
                     /translation="MKLSKYLALLFSCALCFLCACKSTGGPISELLKPNHPITPGHAS
                     IEVPNDAQIEAMREAFFNRPAESEELFRVFVTSDSYTRKQIAHIDTISLKEDKLGDQA
                     IADEFRKFDMINSLQEGQIRVELYPTTGKFYRIRQSKPSVMKETDRVMSDDTRRWEFQ
                     FQKDKIEQKDFRISYQVLLRKKITREQAAKILSEQNKKR"
     gene            2952..5024
                     /locus_tag="PLY93_01755"
     CDS             2952..5024
                     /locus_tag="PLY93_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRP68239.1"
                     /translation="MEIVQVAIYSVACIASFTYALLTVRKFWRLSHLAQPYAIKSTFM
                     QKVNNVIINVLLQKKLMKRPVRGLAHVFIFYGFIVYGAHTTSQIIGGFFGPYTMPWVG
                     TTGYEFSIPELIGKIIPHFAFIYGYALDIFSILVLAGLLFFALRRWVYRAYELDRPSM
                     QSMIVIGMISLLMLSTLVAEGAHALLHSNADISPIRQTVVNMLTAAGITQATAAAWFM
                     GGWWTHIMTVFAFMAFVPNSKHAHLIWAPANFWFEKETPKGQLSYLDTENATVWGSAN
                     VQDFTWKDHLNGLSCIECGRCTIECPANLTGKVLNPKSIMTDLKHALMEQMSKYDAAK
                     KAGKTDEEIAQMPELRVIDNYTSQEALWACTTCYACVEACPVGNNQVDAIVGMRRALV
                     LSEGALPSELQTALTNMENQSNPWGVGAHKREEWTEGLDIKTMAQWKEKGEEPEVLYW
                     VGCAGAFDDRNKKIARSIATVLNKADVKYGILGTEENCTGDSARRAGNEYLFQTLAGM
                     NIQTMNNYGVKKVVTGCPHCFNTLKNEYPQLGGNFEVEHHSTYFEKLVNDKKIELDPE
                     KAKELGSITYHDSCYLGRYNDTYDAPRAVISKATGAKVVEPNDHKQRGLCCGAGGAQM
                     WKEEEKGNERVNLKRTKQLVATEAKTIASACPFCLTMITDGVKELGQEEALRTLDIAE
                     ILERSLVK"
     gene            complement(5319..6968)
                     /locus_tag="PLY93_01760"
     CDS             complement(5319..6968)
                     /locus_tag="PLY93_01760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRP68240.1"
                     /translation="MKRDLQKFQNHTFDILIIGGGVSGASIAWDAALRGYSVALIERK
                     DFGHATSAATSKLIHGGLRYLAQGDIPVVRESLRERRYLEMNMPHQVFPMPFLMPIYD
                     FTPTPRWMLGLGLTLYDLLAWDRNYIDDPDKHLGRKRWLSKEKVLALEPTLNPHGLKG
                     AYHYYDALNRHPNRSNLEYILSAVEKGAVVANYVACDEFIVEENPNGKVVSGVLARDM
                     RSQRAFPIRAKVTVNASGPWGDIVLSKLHQKNEHKLTRSKGIHLLFPRFHKNTTIGLE
                     TRAKEHMFLIPWGNYTLLGTTDTEFKGDPDDVGVTVDDAQQFVSLYNEYFPQQKTLAD
                     VVNMYAGLRPLGAESSVSSTYKASRKHEIVYHGKDDKIGGLVSVFGGKWTTSRALAEE
                     TVDVIEKKFGFTHKPSLSRKTPVVGGNVGGRLAHFIRDAHVRWDHVFSVELIDHLIEI
                     YGALFERVLDYVIKDKSLLRRIDNEYEFIHAEIYYAVEQEMALTLDDFLRRRSGIGNM
                     GPMSDSALEAIAATMGDLLRWDSTRCKAEISQFKKSRSFIS"
     gene            complement(7019..8854)
                     /locus_tag="PLY93_01765"
     CDS             complement(7019..8854)
                     /locus_tag="PLY93_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRP68241.1"
                     /translation="MTTLNALLFAEENTVEDILANTIHEAGFSFAMTHIEDEKQLHDA
                     LSQKWDFFFYTLSSGENKIPLSVVQEKIRALLLELPLILILVNPEQEHEDIRAVKEGA
                     SDYFFLDKPERCRAILWREVRNQKKVQVIEEDYDTQLLQLHLLKAAVENINTGLVITD
                     ARQAERPVVYLNKRFEEISGYSSIEILGQSLRVLYGSEKNQPGFIELQKRLSHFEGGE
                     FYLHPYHKDGTRYSAREHISPIRNSEGRVQYFVIVHEDITKKKALEETLAEAWERTQL
                     VLYASKMGYIDANLTNGKIYYSSRYADILGYKQYEMHQLKINWLDLVHEDDKAELAQQ
                     IHAATQFKSHVIDHECRFVRKDNSLVWVRLRGRIIEHDLAGNWQRYVGTILDISERRA
                     QQERITDLTQKLMYVSEAELEKIASVLHDSVGQSLVLLKLNALQFLDRHQLRNEENIR
                     ALVDPITGVLEQVRHLSHSLTPIHMEQIGLDLVLEDMLDSASHLSGVQIQSRLSELVD
                     FFPNNWSLEFYRIVQEIITNILKHAEATRIRINASRDEKNLIFFITDNGRPFDSTKVK
                     NSFGMQMLNERIRHLNGKITFSAEKEGTTRTIVVPHRVLNGLRHV"
     gene            complement(8873..9730)
                     /locus_tag="PLY93_01770"
     CDS             complement(8873..9730)
                     /locus_tag="PLY93_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP68242.1"
                     /translation="MTNSVGQCLTFQIRGQVFGIDILHVKEIIEYGGVTKVPLVPTFI
                     HGILNLRGSVVPIVDVGGRFGWDIITPSRLTCIVVTEVEHEGARLHIGLLVDAVNEVV
                     SLTAENLESAPQFGANVRADFIKNIGKIGSKFVLLLDIAKLLDIEAVARSSSGGYTRM
                     AKKILTVDDSITMRRMIKMTLEKSGYKVHEAGDGLEGLSAVLKVAPDLIISDVNMPNM
                     KGIEFVKTLREDAAYVAHKFVPVLMLTTENSDAMRAAGASAGAQSWMQKPFTPDKLTM
                     AVEKLIGEA"
     gene            complement(9752..>10061)
                     /locus_tag="PLY93_01775"
     CDS             complement(9752..>10061)
                     /locus_tag="PLY93_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68243.1"
                     /translation="SDLVQEIKTASQQQKDGVDQMQRSVHHLDSLTQGNAASSEELAS
                     TAEELHAQAQSLWDGVSFFKLEEGDDTIHHLKESIKSKPIVTKTVRARDNGEDFIAFD
                     "
BASE COUNT         2683 a         2426 c         2303 g         2649 t
ORIGIN      
        1 cacacctttg ctatttaatt tcacgcgggt taaaaacatt cccattgcgt atgcgcgagc
       61 acaaccgctc cacactcgcg attgcaacat ggcttgaaaa gcatcccaag attgagaaag
      121 tatggtatcc cttactccct tcacacccgg attatgcact cgcgcaaaaa tatttatcgg
      181 gcggcggcgg cgtggtgagc ttttgcatca aggccgagga cgtctcgcaa aaaacgcgca
      241 tgactcttgc gaacactttt ttgaaccgct tgaaaatccc caccatcgca gcgtcgttgg
      301 gcggggtcga gtcgctcatc caccacgtcg ccatcatgag ttacgccgat ttaactcctg
      361 aaaaacgcga agaaatcggt atcccggaca acctcattcg gtttgctgtg gggttggaag
      421 acaccgacga tcttatcgct gacttaacgc aggcgttggg gtaatcctcg ccttatttcc
      481 accacaacga cagccacggt atatacgtga tggcgatttg cgccaagagt aaaaccagta
      541 aataagggat aatggatttg tagatttcgt tcatcggttt ttggaaacgc aacgagctca
      601 aaaaaagatt gagtcccacc ggtggggtca aatagccaat ttctaaattg gtgagaaata
      661 aaatccccaa gtgcagcatc gggatttggt agctttcagc aatcgggatg atgagcggca
      721 caataacaat aatagcagag aaaatatcca tcaacgaacc gacgatgagg aggaagatat
      781 taagcactat taaaaacgtg agaggagaat ggatgtattg gctaataaag ccaaagagct
      841 tggcgggcac tccgtcataa agaagcgcgt tggttaaaat catcgcgaag aaaatgatga
      901 ggataatcga gcccaccatg gcgatggctt cttcaaacac cgccatgagt ttttggaaac
      961 gaatttcacg aaaaatcacg acttctaaaa gaaaatagta aagaagaaaa aacactccca
     1021 gttcggtgat gatcatgtaa cgcgagtgca tcaataaata gagaagtgga atagccgcaa
     1081 tttctaatgt gagctttacc gcgtcgctgc gcgtaaaaga aatatgcgct gtcgatgtat
     1141 gcagcgtatt tgcaacgctc atttttttac tgaacgaata gaaaaagaca accaacgaga
     1201 gcgataacag aagcacgata cccggcaaaa cacccgaacg gtatagttcg ttgatgtcgg
     1261 ctttgttgtt gctcatgatg ccgaataaaa tgaccggcaa actggggctg aacaaaagac
     1321 caatcgaacc cgacgcggtg atgagaccca acgcacgttt ctcgctatac cccgcgccgg
     1381 ttaaaatggg gaataagatg ccgccgatgg cgacaatcgt taccccagac gcgcccgtga
     1441 gcggcgtgaa aagcgcggcg attaaaatgg cgataagccc caagcctacc gtgcccactt
     1501 tgccgacgcg gcggatgacg atgaggtata agttcaccac ccgcgccgcg aattgcgaac
     1561 gcgcgataag ataccccgtg agagtaaaat aagggatagt taaaaaaagc gcgctttggt
     1621 cgccggtgag tttagaaagt ggatcgctaa agacgacggt gagctgacgc gtcaaatcgc
     1681 ctaccgtcgt cgatcccaat tcgatggcat tggtggtttg gtaaagcgtg cccagtgtgc
     1741 ccaagccaat aaaaagtggc aggccaaaaa ggagcgcgac aacaccgata acgccaacgg
     1801 ctacaatcat ggttcttcta agagtttaac ttttttacca aattcacggc taaacgtttt
     1861 tatcatctgc gccgcttctt tgtcttgggt tgcgcggtct aaaacgtccg ccattttttt
     1921 tagcacttga taaaagaaaa tgttcatgtc tcccacaagc atgtctttgg tccacagatc
     1981 aatccccaac gttgtttttt cttcgccgct aaaaagagag agcataaatg cttctgcctt
     2041 ttcttcgcct ttcgatgcgc ccggggcatc ccaagtgatc gactcgggga ttgcgttttc
     2101 gtctaatacg acacgcagcg taatttcttt tttttcagac actatttacc tctttttatt
     2161 ttgctcgctt aaaattttag ccgcttgctc gcgggtgatt tttttccgta acagcacctg
     2221 gtagctgata cgaaaatcct tttgctcgat cttgtctttc tggaattgga actcccaacg
     2281 tcttgtgtcg tcgctcatca ctctgtcggt ttctttcatc accgatggtt ttgattggcg
     2341 tatacgataa aatttacccg ttgtgggata aagctccact cgaatttgtc cttcttgaag
     2401 gctgttaatc atatcaaatt tacgaaattc atcggcgatg gcttgatcgc ccagtttgtc
     2461 ttctttgaga gagatggtgt caatgtgtgc aatttgtttc cgcgtatagc tatcggacgt
     2521 gacaaaaaca cgaaagagtt cttcgctctc agcgggacgg ttaaaaaaag cttcgcgcat
     2581 cgcctcgatt tgtgcatcgt tgggcacttc gatggaggca tggcctggag tgattggatg
     2641 gttgggtttc aataattctg aaattgggcc tccggtggat ttacacgcgc acaggaagca
     2701 gagtgcgcaa gaaaatagta atgctaaata ctttgacaac ttcattagat gagaagcaaa
     2761 taatctaaac cgcccgtcaa acaaaaggta attattctgt atgtggttca acacatgcgt
     2821 tccatggccg taagctttcc gactttcgca ggaggtaccg tcgttgcatt caaaaacata
     2881 gggtgcggcc taaatgtcag tgatttgtca gaatcttctt tacacctgct cttcattctc
     2941 caataaaacg catggaaata gttcaggttg cgatttattc cgtcgcgtgt attgcttctt
     3001 tcacttatgc ccttcttacc gtacgtaagt tttggcgtct ttcacacctc gctcagccct
     3061 atgcaattaa atcgactttt atgcagaaag tcaataacgt tatcatcaac gtccttttgc
     3121 aaaaaaagct gatgaaacgt cccgtccgtg gacttgcgca tgttttcatt ttctatggct
     3181 tcatcgtcta cggtgcacac accacgagcc aaattatcgg cggctttttc ggcccttata
     3241 ccatgccttg ggtcggtaca accggctatg agttttccat cccagaactc atcggtaaga
     3301 tcatccccca ctttgctttt atttacggtt atgcactcga tattttctct atcctcgttt
     3361 tagccggtct cctctttttt gcgctgcgcc gctgggttta ccgtgcgtac gagctcgatc
     3421 ggccatcgat gcaatcaatg attgtcatcg gtatgatttc actcctcatg ctttctacgc
     3481 ttgtagcaga aggcgcacac gctcttttac attcaaatgc tgacatttca cccatccgcc
     3541 aaactgttgt gaatatgctc accgcagccg gaattacaca ggcaactgct gccgcatggt
     3601 tcatgggtgg ttggtggacg cacatcatga cggtgtttgc gtttatggcg tttgtcccca
     3661 actccaagca cgcgcacctg atatgggcac cggctaactt ctggtttgaa aaagaaaccc
     3721 ccaaaggcca actttcttac ctcgacaccg aaaacgccac cgtttggggg tcggcgaacg
     3781 tgcaggactt tacatggaaa gaccacttaa acggcctaag ctgtattgaa tgcggtcgtt
     3841 gcaccatcga gtgccctgca aatttaactg gtaaagtttt aaaccccaag tcaatcatga
     3901 ctgacttgaa acacgcgctc atggaacaaa tgagcaaata cgacgcggca aaaaaagcgg
     3961 gtaaaacaga cgaagaaatt gcccaaatgc ccgagctacg tgtgatcgat aactatacct
     4021 cgcaagaagc attgtgggcg tgtaccactt gctatgcgtg cgtggaagcc tgccctgtgg
     4081 gtaacaacca agtcgacgcg atcgtaggta tgcgccgtgc gcttgtactt tccgaaggcg
     4141 ctctcccgag cgaactccaa accgctctca ccaatatgga aaatcaatcg aacccatggg
     4201 gtgtgggtgc gcacaagcgc gaagaatgga ctgagggctt ggacatcaag accatggcgc
     4261 agtggaaaga aaaaggtgaa gagcccgaag tcttgtactg ggtaggttgt gccggagcat
     4321 tcgacgaccg caacaaaaaa attgcccgtt cgattgctac cgtcctcaac aaagccgacg
     4381 ttaaatacgg cattttaggc accgaagaaa actgtaccgg cgactcggcg cgccgtgcag
     4441 gaaacgagta tttgttccaa accctcgcgg gtatgaatat ccaaacgatg aacaactacg
     4501 gcgtgaaaaa agtcgtcacc ggttgcccgc actgcttcaa caccctcaag aacgaatacc
     4561 ctcagttggg cggtaatttt gaggtggaac accactcgac ctattttgaa aagctcgtga
     4621 acgataagaa aatcgaactc gatccggaaa aagcaaagga gttgggctcg atcacgtacc
     4681 acgattcgtg ttatttaggc cgctacaacg acacgtacga cgcaccacgc gcggtcatat
     4741 ccaaggcaac cggcgctaaa gtcgttgaac ccaacgacca caagcagcgc ggcctttgct
     4801 gcggcgcagg cggcgcacag atgtggaaag aagaagaaaa gggcaacgaa cgcgtgaact
     4861 tgaaacgcac caaacaactt gtggcgactg aggcaaaaac aatcgcaagc gcatgccctt
     4921 tctgcttaac gatgatcacc gatggtgtaa aagagctagg tcaagaagaa gcgctacgca
     4981 ctctcgacat cgccgagatt ttagagcgta gcctcgttaa gtagaatatg taaatcgggc
     5041 tggttaataa gtcgcctgcg tgcctattag ccagccagcc actgtgagcc tgtgtgtaat
     5101 actggcacag tatttttgca agttgaagta acaagctgcc aaggtgacta tgtcgatttt
     5161 ttgatttatg tcacattagg gggtaactat ccacgggggc gctggagtgg tcgaaaatca
     5221 gggtgaaatt taagaaaaag cttctttata tatgcgtaat ctattatgcg taatctatcc
     5281 atatagctga cagcgcttgt tgtatgcttg cgttcggatt atgaaatgaa actacgtgac
     5341 tttttaaatt gagaaatttc tgctttgcaa cgggttgagt cccaacggag taaatctccc
     5401 atggtcgcgg caatcgcctc taaagcgcta tcgctcatgg gccccatgtt accgatgccc
     5461 gaccgccggc gcaaaaaatc gtcgagggtg agcgccatct cttgctcaac ggcgtaataa
     5521 atttcagcgt ggataaattc gtattcgttg tcgatacgcc gtagcaacga tttatcctta
     5581 atcacatagt cgagcacgcg ttcaaaaagc gcaccgtaga tttcaataag gtggtctata
     5641 agctcgacag aaaaaacgtg atcccaacgc acatgcgcgt cgcggatgaa gtgcgccaag
     5701 cggccaccga cattgccacc cacaaccggt gtcttgcgcg aaagcgaggg tttgtgcgta
     5761 aaaccaaatt tcttttcaat gacgtcgacc gtctcctcgg cgagcgcccg agaggttgtc
     5821 catttaccgc caaaaaccga aacaagaccg ccgattttgt cgtctttacc gtggtacaca
     5881 atttcatgtt tgcgtgacgc tttgtaagtg ctcgagaccg aactttctgc gcccaacggg
     5941 cgtaaccccg cgtacatatt cacgacatcg gcgagcgtct tttgttgagg aaaatactcg
     6001 ttataaagcg acacgaattg ttgagcgtca tcgacagtca ctcccacatc gtctggatct
     6061 cccttaaact cagtgtcggt cgtacctaag agggtatagt taccccacgg gattaaaaac
     6121 atgtgttctt tagcacgagt ttcaagacca attgtggtgt tcttatgaaa acgggggaac
     6181 aaaagatgaa tccctttgga acgcgtcagc ttatgttcgt ttttttgatg tagttttgaa
     6241 agaacgatat cgccccacgg gcctgacgca ttcaccgtca cttttgcccg aatagggaaa
     6301 gcacgctggc tgcgcatgtc acgcgccaac acaccgctaa caaccttacc gttaggattt
     6361 tcttcaacga taaactcatc gcaggcgaca tagttagcga caaccgctcc tttttcgacc
     6421 gccgataaaa tatattctaa gttggatcga ttggggtggc ggttcagagc atcgtaataa
     6481 tgatacgccc ctttgagacc atgcggattg agcgttggtt ctaatgcgag gactttttct
     6541 ttagaaagcc agcgtttacg cccgagatgc ttatcggggt cgtcgatata gttgcggtcc
     6601 cacgcgagta aatcgtaaag cgtgagcccc aagcccaaca tccaacgtgg tgtgggtgtg
     6661 aaatcatata tcggcatcaa gaatggcatc ggaaaaacct gatgcggcat attcatttcc
     6721 agatagcgcc gctcgcgcag cgattcgcgc acaacgggta tatcgccttg cgctaaataa
     6781 cgcaaaccac catggatgag ttttgaagtg gccgccgacg tggcatgacc aaaatctttg
     6841 cgctcgatga gtgcgacaga gtagccgcgc agcgcagcgt cccacgcgat ggaagctcca
     6901 ctgacaccac caccaataat taaaatatcg aaagtgtggt tctggaattt ttgcaagtca
     6961 cgtttcattg cacgtttgag aagccccgca tacgcagaat aagcaacgct attttttgtt
     7021 aaacatgccg taaaccattt agaactcgat gcggaacaac aatggtgcga gtggttcctt
     7081 ctttttcagc gctaaaggtg atcttaccgt tgagatgacg gatacgttcg ttcagcattt
     7141 gcatcccgaa actatttttg acttttgtgc tatcgaaggg gcgtccgtta tcggtaatga
     7201 aaaagattag atttttctcg tctctgcttg cattaatgcg gattcgtgtc gcttccgcgt
     7261 gtttcaaaat gtttgtgata atttcctgca caatacggta aaattccaaa ctccagttat
     7321 tgggaaagaa atccactaac tcgcttaagc gtgattgaat ttgaactccc gaaagatggg
     7381 aggcgctatc gagcatatct tcgagcacca agtcgagacc aatttgttcc atgtggatgg
     7441 gagtcaacga atgcgacaag tggcgtactt gttctaacac gccggtgatg gggtctacca
     7501 atgcgcgtat attttcttca ttccgtaact ggtgtctatc taagaattgt agcgcattga
     7561 gctttagcaa cactaaactc tggccaaccg agtcgtgcag tacactcgca attttctcaa
     7621 gctcagcttc gctgacatac atgagctttt gggttaaatc agtaatacgc tcttgctgcg
     7681 ctcgacgctc tgaaatatcg agtatggtac caacataacg ctgccaatta cccgccaaat
     7741 cgtgttcaat gatacgccca cgcagacgca cccacacgag gctgttatct ttacgcacaa
     7801 agcggcattc atgatcgata acgtgactct tgaattgagt cgctgcatga atttgctggg
     7861 ctaattcagc cttatcatct tcgtgaacca aatcgagcca attgattttt aactgatgca
     7921 tttcatactg cttgtaccct aaaatgtcag cgtagcgtga gctgtaataa atcttaccgt
     7981 ttgtgagatt agcgtcaata tagcccattt ttgacgcata gagaacaagc tgtgttcttt
     8041 cccatgcctc ggctaaagtc tcttcaagcg ctttcttttt agtaatatct tcatgcacga
     8101 tgacaaagta ttgcactcta ccttctgaat tacgtatggg agaaatatgc tcgcgcgcag
     8161 aatagcgggt gccgtcttta tggtaggggt gtaaataaaa ctcgccaccc tcaaaatggg
     8221 acaacctctt ctgcaactcg ataaacccag gttgattttt ttcagaccca tacagtacac
     8281 gcaaactttg gcctaaaatt tcaatactcg aatatcctga aatttcttca aaacgtttat
     8341 tcaaataaac aactggacgt tccgcttgcc tggcatcggt gatgacaagt ccagtattga
     8401 tattttctac cgctgccttc aaaaggtgta gttgcaataa ttgagtgtca tagtcttctt
     8461 cgataacttg tacttttttt tgattacgta cttcgcgcca taaaattgca cgacaccttt
     8521 ccggtttatc taaaaagaaa taatcgctcg caccttcttt taccgcgcgg atgtcttcgt
     8581 gctcctgctc tgggtttacg agaatgagaa tgagaggcag ttcaaggagc agcgcacgta
     8641 ttttttcttg aacaacgctc aacggaattt tattttcacc ggaagaaagt gtgtagaaga
     8701 agaaatccca cttttggctc aaagcatcgt gtaattgttt ttcatcctcg atgtgagtca
     8761 tcgcaaaaga aaatccagcc tcgtggatgg tattggccaa aatgtcttcg acagtatttt
     8821 cttcggcgaa gagtaaagcg ttaagagtgg tcatcgtctt cgaaaaattg catcacgctt
     8881 cgccaatgag tttctcaacc gccatcgtta acttatccgg agtaaaaggt ttttgcatcc
     8941 aactttgcgc gccagcactt gcccccgccg cacgcatcgc atcgctgttt tcggtagtga
     9001 gcatcaacac cggcacaaat ttatgcgcga cgtatgcagc gtcttcgcgt aatgttttaa
     9061 caaattcaat ccctttcatg ttcggcatat tcacatcgga aataattaaa tcaggagcaa
     9121 ctttgagcac cgcgcttaat ccctctaaac catcgccagc ttcgtgtact ttatagccgc
     9181 ttttttctaa agtcatttta atcatacggc gcatcgttat cgaatcgtcg acagttaaaa
     9241 ttttttttgc cattcgtgta tatcctcctg agcttgatcg cgcaactgct tcaatgtcta
     9301 aaagttttgc gatgtccaac aataaaacaa atttactacc aattttgccg atgtttttga
     9361 tgaagtcagc gcgtacattc gccccaaatt gcggtgcgct ttctaaattt tcagcggtga
     9421 gactcaccac ctcgtttaca gcatcgacta aaagaccaat gtgcaaacgc gcgccttcgt
     9481 gttccacctc ggtgaccacg atacacgtta gccgcgatgg ggtgatgatg tcccaaccaa
     9541 agcggccgcc cacgtcgaca atcggcacca ccgaaccgcg taagttcaaa attccgtgga
     9601 taaatgtggg cacgagcggc actttggtga cgccaccata ctcaataatt tctttgacgt
     9661 gcaaaatgtc gataccgaac acctgcccac gaatttgaaa cgtcaaacac tggcctacgc
     9721 tattcgtcat attaactttt attgtttcct ctcagtcgaa cgcaataaaa tcttcaccgt
     9781 tgtcgcgcgc gcgtactgtc tttgtgacga tcggctttga ttttatactt tcttttaggt
     9841 gatgtatagt gtcatctcct tcttcgagtt taaagaagct gacgccatcc cacaaagatt
     9901 gcgcttgtgc gtgcaactct tctgcagtcg aagcgagttc ttctgatgaa gcggcgttgc
     9961 cttgggtaag cgaatcgagg tggtgtaccg aacgttgcat ttgatcgaca ccgtcctttt
    10021 gttgctggga tgcggtttta atttcttgca ccaagtcaga t
//
LOCUS       DAOMPD010000027         9802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_2986_length_9802_cov_2.576998, whole genome shotgun sequence.
ACCESSION   DAOMPD010000027 DAOMPD010000000
VERSION     DAOMPD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_9802_cov_2.576998"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /gene="secA"
                     /locus_tag="PLY93_01780"
     CDS             complement(<1..400)
                     /gene="secA"
                     /locus_tag="PLY93_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780757.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRP68244.1"
                     /translation="MLQFFLKAIFGSKYERDLKKMRPLVERINSMEPEMQRLSDAELA
                     AKTPAFKERLANGEKLIALLPEAFATLRETSVRTMKMRHFDVQLMGAIALNAGKIAEM
                     KTGEGKTLTATLAVYLNALVGRGVHLVTVND"
     gene            595..2064
                     /locus_tag="PLY93_01785"
     CDS             595..2064
                     /locus_tag="PLY93_01785"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP68245.1"
                     /translation="MANTAIKPDVKILIIGAGFSGLGMAIKLRDAGIEDFLIVEAASE
                     VGGTWRDSKYPGAAVDVPSHLYSFSFEQTNWSRMFAKSEEIQRYIVDTADRHNLRSKI
                     KFNTKIVEAKFDEANGLWEAKTAKGETMRARYVVSAIGPFAGSNIPNIPGVKDFKGET
                     VHTSSWNPKLDLRGKRVAVVGSGASAIQVVPAIQPLVKELKVFQRTPSWIVPKADYEY
                     SELEKFVYKYLPFTQKIRRGFIFGLTELLATAIVWDTPMTTFLENVCKRVINNKITDP
                     ELRRKVTPNYRLGKTRMLISNDWYPALAQANVELVDHGVESVTEKGLKANGKEYEVDV
                     IVWATGYKSPSQGFPFPLTGVQGRDLNTYWKDGAKGFKGVAIAGFPNLFTLMGPNTGP
                     GHTSVLVYTEAQMDYARQAIQACEEKQIKSVAVSDETQDQFNEELYKKMKRTTWGNGG
                     ESWYYAKNGLNTTLYPGFATEYVMSVRNFDLNDYQVKFA"
     gene            complement(2114..2497)
                     /locus_tag="PLY93_01790"
     CDS             complement(2114..2497)
                     /locus_tag="PLY93_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68246.1"
                     /translation="MVPKSKRILIIDDSALIRELLTQVVEALDFSTEVRVAKNGTDVV
                     ELFQKGYTFDLVTLDMNMPDMDGIHILKKIRLFDDAVKVIVISSNLKTSRTDEYIHLG
                     VEHFAAKPFDSDELKKQLTKIFEEE"
     gene            complement(2539..3393)
                     /locus_tag="PLY93_01795"
     CDS             complement(2539..3393)
                     /locus_tag="PLY93_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68247.1"
                     /translation="MKIYFDTGSGNQELNFSLEATLPFIEILKKLTEWAESQKVFIVD
                     YRIAAKPEFVENENLNSDEIDSIYLRLGDQTTLIESNLRELIDYTDRAGIHLAKTIQE
                     ARALSEQEKGDLNSGAAFIAESLEALSRHLRPESVEYMERAMNALLHQDDLIEKVNAL
                     GAVQHQLKLWLRQTEFSRITAEEAKEKVTRFREQISDLSHDLEQIAARFTQGKEQEAL
                     SKLEMVSQILVDAAMLMRIAKDSKSALVDKLVSLLGDLTQAVTARDLVTAADIADFDL
                     RDTLQEIA"
     gene            3496..5037
                     /locus_tag="PLY93_01800"
     CDS             3496..5037
                     /locus_tag="PLY93_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRP68248.1"
                     /translation="MKNIVLCLLLSLACAIIPLNAAPTLENAAVEFSNFFKENSQKTV
                     AVLPFVSKAAFETTAIQTKFIEALVASGKIIVIERDRIAQLLNEYQIEQSGLTDKERI
                     GRLLQADYLVLGNAAAKSKTVFEVNARLVDVATSQIVKTVNFEAERNEIEGPYPVHNP
                     YPSKKFLGEPLVQLAILLDTSSSMDGLLNQTRTQLWKIVNTLAGGEREGKKPRIEVAL
                     YEYGNSMLKESNHYVRQVLPFTTNLDQASEKLFELKTMGGLEYCGAALNDAFTNLEWK
                     NYDDVYRVVFIAGNEPFTQGPIPFKGALEKARQKGIFVNTIFAGSRQQGIATQWLSAA
                     RLAYGDFRVIDQNQMDQVMATPYDEEIRNLGEAYNATVIPLGEVGVIAQKRMQTQDRK
                     IAATGVENGALTERAQAKATEQYSAAHHWDLTSITHPDKALEEINKNDLPQTLREKPQ
                     GQIKAYVLQKQAERKKIQKRIDALTQKRNVYIGNASKSNRNDDFGRATQVTIRAQGKK
                     RGFIF"
     gene            complement(5045..6493)
                     /locus_tag="PLY93_01805"
     CDS             complement(5045..6493)
                     /locus_tag="PLY93_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68249.1"
                     /translation="MIKVSLRCLYSAAFFIPISIVFAAPQPAAKSGLAVVAIPQYIDT
                     TASKNYGYLSGSLTDAVNQSMQEKFDYSRANQTAVDAKTKQLWKKNQTPTDLEIKQLA
                     ISTQSDYVVVGSYSLTPNKKQIVFNTRIYIAPDKFISIAPLTNNADATLFDATNKVAT
                     EIVKEIEREALARATAQKSTPTKEGEKIALSKNENKSVAPAPKKEDDSYNYLRENDKR
                     HLNITAGYASNTVERQALFYQYPASAMYLRADARLQEELLLYAHLTWPSTDVKDTMPT
                     NYKNNANQTIGLEYGNRNEGYMVGLNYRNFSGTNPAGIFQRDLLNWSSSLYMKTSFHI
                     FNIQSISFGLDLDSYMDIFSIKQIDPTLSTTESDVGLLWNVEAAVRAGYYFSPLGVFA
                     VASVGGAYSIIDSTNRPMNAALTQTIDRSMSFVGLRYSMSLQKWFKNIRLKIAGYLTP
                     SLGADYQYLNSKNEKQTSLAYFDVSATYQFEF"
     gene            complement(6615..7793)
                     /locus_tag="PLY93_01810"
     CDS             complement(6615..7793)
                     /locus_tag="PLY93_01810"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP68250.1"
                     /translation="MSSTSHSYRNRVIRLALFLLVGLSLVFLFPSAQFRLTPYFLYGV
                     LWCIIIATVFWNGLLHALVVQLSIIAMLVFLIAFDLKYRSLDLKLLVVWQSVIFLSLC
                     AIGFLLLERSELREKVASQKKLAQELEGEKLNEINRLLAADHAVSFEELTILFSDIRG
                     FSSIAEVLPANTLYMALNVYYEGVVEIVEQHGGIIDKFLGDGIMVVFGLGASKKNAPL
                     KAIQCAIQMQEYQKGIEKISLPGKIQIGTGFGIATGRVMVGLVGATSRYEWTCLGDPV
                     NLSARLEQFTRKLPTDTLVSETTMMQLPENSGVMARAVGYFKVRGRFTEETIYEVFNN
                     DAEHIREGKNKTKEKFEKALKMQRRGFVDDARAIFREIHREYPDDLIARHYATNETVR
                     "
     gene            complement(7768..9312)
                     /locus_tag="PLY93_01815"
     CDS             complement(7768..9312)
                     /locus_tag="PLY93_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRP68251.1"
                     /translation="MKSFIERLLNASIATKFIGVTTSIFLIAIAFASVSIRSMQKKSA
                     VREAHLFADSTEKMALSGLTTLMITGQMEHREQLLSQINETGIVSDLRIISGPKVIAA
                     YGAGRESEKAKNEYENAVIRTGVIHTENENDEYLRIVKPIHAQANYLGKNCLACHQVK
                     PNEVLGAITMRVSLVEITKQSRRLSLEIAGVGLVVVAILIIGILLVSRFIISTPLEQV
                     LLSFQRLQQKDYTIKINPRYRDEIGRVGDGVNTFIEYSVELLTKLGKISHQLSSLANK
                     TRETAENFLRLSERQANVIEETSQAVEDLNTSLSTEKDELKDFHSLVRRTVEYAVAWG
                     DMYHKLSGKTASFRDAYEENTHDFKVVYKNLNDAIGNAETIFDEKIKEGQNNNLTEIK
                     TLQKLIADLSGVAVLLKRTLLMHIKASDSYMQTRNQLDEFAQSIDENEKDIGKLTTLH
                     DQIVEEAEAQNSASGEISKTISILSTEASSTAATAMDLTQMAHEASRLALLLKDLLDE
                     FNLPQL"
     gene            9598..>9802
                     /locus_tag="PLY93_01820"
     CDS             9598..>9802
                     /locus_tag="PLY93_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRP68252.1"
                     /translation="MQHNNYKPATLAVHGGERTYDGSPHTPVFNTTTFAFENTAALLD
                     VVDGRKTGPLYTRYGLNPSITSLE"
BASE COUNT         2645 a         2182 c         2239 g         2736 t
ORIGIN      
        1 aatcgtttac cgtcacgagg tggaccccgc gccccacaag cgcgttcaag tacaccgcga
       61 gcgtcgcagt caatgtttta ccctcaccgg ttttcatttc ggcaatttta ccggcattta
      121 aagcaatcgc gcccatgagc tgcacgtcga agtggcgcat tttcatggta cgcaccgagg
      181 tttcgcgcaa ggtcgcaaac gcttcgggta ataatgcaat gagtttttcg ccgttggcta
      241 aacgttcctt aaaagcagga gttttggccg ccagctcagc gtcacttaaa cgctgcatct
      301 caggttccat gctattgatg cgctcgacga gggggcgcat tttctttaaa tcgcgttcgt
      361 atttagaacc gaaaatcgcc ttcagaaaaa attgtaacat tgggtaattt ttgggtgaaa
      421 gacacggcgt cgacgcaaaa ccagtatagg cgtagaccgc cgcacaatgg agccaaaaaa
      481 aataacgtgg cgcacaacga aaagcgaaca aggtttcact tttttattta caatataaat
      541 ccgccttttt gaatacgctg aaatggtggc caaggccatc tatttggagg aattatggca
      601 aacacagcaa ttaaacccga tgtaaaaatt ctcatcatcg gtgcgggctt cagcgggctc
      661 ggtatggcaa tcaaactacg cgatgcaggc attgaagatt ttttgattgt tgaggcagca
      721 agcgaggtgg gcggcacttg gcgcgacagt aaatatccag gggcagcggt agacgttccc
      781 agccacctgt atagtttttc gttcgaacaa acgaattggt cgaggatgtt cgcgaaatcc
      841 gaagaaattc aacggtacat tgtcgatacc gccgatcgtc ataatttacg ctcaaaaatt
      901 aagttcaata caaaaattgt agaagcgaaa tttgacgaag ccaatggttt gtgggaagcc
      961 aaaactgcaa aaggggaaac gatgcgcgcg cgttatgtcg tcagcgctat cggccctttc
     1021 gcagggtcga atatccccaa catccctggc gttaaagatt ttaaaggcga aaccgtacac
     1081 acttcttcat ggaatcccaa gctcgacctc agaggcaaac gagttgcagt tgtgggtagt
     1141 ggcgctagtg caatccaagt ggtgcctgca atccaacctt tagtaaagga acttaaagta
     1201 ttccagcgca ctccatcgtg gattgtcccc aaggcagact acgaatacag cgagctggaa
     1261 aagttcgtgt ataaatatct cccgtttaca caaaagatcc gtcgtggttt tatttttgga
     1321 ctcaccgaac tactcgccac agcgattgtg tgggatacac ccatgaccac gtttttagaa
     1381 aatgtctgta aacgtgtcat caataacaaa atcacagatc ctgagctgcg ccgcaaagta
     1441 acccccaact accgtttggg caaaacgcgc atgcttatct caaacgattg gtatcccgca
     1501 ttggcgcagg cgaacgtcga acttgtcgat cacggcgttg agtcggtgac tgaaaaaggt
     1561 cttaaagcga acggtaaaga atacgaagtg gacgttatcg tttgggcgac tggctacaag
     1621 tcaccatcgc agggttttcc tttcccgctc accggtgttc aagggcgcga cctcaacacc
     1681 tactggaaag acggcgcgaa aggttttaaa ggcgtagcga ttgcgggctt ccccaatctc
     1741 ttcacactca tgggacccaa caccggcccc ggccacacct ctgtactcgt ttacactgaa
     1801 gcgcagatgg attacgcacg ccaagcgatt caagcgtgcg aagaaaagca aattaaatct
     1861 gtcgcagtga gcgacgagac tcaagatcaa ttcaacgaag aattatataa gaaaatgaag
     1921 cgcacaacgt gggggaacgg gggtgaaagc tggtattacg cgaaaaatgg tttgaacacc
     1981 acgctttatc cgggctttgc taccgaatac gtgatgagcg tacgcaactt tgatttgaac
     2041 gactaccaag taaaatttgc ttaacttaaa tccacgctta ctgcacacgg tgtgcgcagt
     2101 aagcgtgaag attttattct tcttcaaaaa tttttgttaa ttgcttttta agttcatcgc
     2161 tatcgaatgg ttttgcggca aagtgttcta cgcctaaatg tatatattca tcggtacggc
     2221 tcgttttcaa gtttgaacta attacaatca ctttcaccgc gtcgtcaaac aaacgaattt
     2281 tttttaagat atgaattcca tccatatcgg gcatgttcat gtcgagtgtc actaaatcga
     2341 atgtgtatcc tttctggaaa agctcaacca catcggtacc gtttttcgcg acacgcactt
     2401 ccgtcgaaaa atccaacgct tcaaccactt gggttaggag ttcgcggatt aacgctgaat
     2461 catcgataat taaaatacgt tttgatttcg gcaccatgag agactaaatt taagagtcta
     2521 tgcggcaacc gtaaagcttt atgcaatttc ttggagtgta tcgcgtaaat caaaatcggc
     2581 aatgtccgcg gcggtcacta aatcgcgcgc ggtcactgct tgggtcaaat cgcccaagag
     2641 cgaaaccaat ttatccacaa gcgcgctctt ggagtctttg gcgatgcgca tgagcatggc
     2701 ggcatcgact aaaatttggg aaaccatctc caattttgat aatgcttctt gttccttgcc
     2761 ctgggtgaag cgggcggcga tttgttcgag gtcgtggctc aggtcggata tttgttcgcg
     2821 gaagcgtgtg actttctctt ttgcttcctc ggcggtaatc cgtgaaaact ccgtctgccg
     2881 caaccatagt ttaagttggt gctgcactgc gcccaaggca tttacttttt caatgagatc
     2941 gtcttgatgg agaagggcgt tcattgctct ttccatatac tcgacgcttt ccgggcgtaa
     3001 gtggcgcgac agcgcttcca gcgactctgc gatgaaggcg gctcccgagt tcaaatctcc
     3061 tttttcttgt tcgcttaatg cgcgtgcctc ttggattgtt tttgctaagt gtatacccgc
     3121 tctgtcggta tagtcgatta actcgcgcaa attcgattcg atgagcgttg tttggtcccc
     3181 caagcgtaag tagattgagt ctatttcgtc ggaatttaag ttttcattct cgacaaactc
     3241 aggtttagcg gcgatacggt aatcgacgat gaaaactttt tgtgactctg cccactcggt
     3301 gagttttttg agtatttcga taaaaggcaa cgtcgcttca aggctgaaat taagttcttg
     3361 atttcctgag cctgtatcga aatagatttt catatttaag taacgacttc tttacagaat
     3421 ttcccaagcg ttttaatttt gaaaaaaatg aacgtctatg tatttttttc gtctaagaga
     3481 atagaccgag ggattatgaa aaatattgtt ctttgtttgc tcctttcgct tgcttgtgca
     3541 ataataccac ttaacgctgc accaacgtta gaaaacgccg ccgttgagtt ttcaaatttc
     3601 tttaaagaga actctcagaa aacggtggcg gtgctaccgt ttgtcagcaa agcagctttt
     3661 gaaaccacgg cgattcaaac aaagtttatt gaagcgttgg tggcatcggg taaaataatt
     3721 gtcattgagc gcgaccgtat tgcacaatta ctcaatgaat accaaatcga gcaatcaggg
     3781 ttaaccgata aagaacgtat aggccgtctt ttacaagcgg attatcttgt cttggggaat
     3841 gccgctgcga aatcaaaaac tgtgtttgag gtgaacgctc gcctcgtcga tgtggcaacc
     3901 tcacaaattg tgaagacggt aaattttgaa gcagaacgta atgaaattga gggaccgtat
     3961 cctgtacata atccataccc gtcaaaaaaa tttttgggcg agccgttggt tcaactggca
     4021 attttgttgg atacttcctc gagcatggat ggtcttctca accaaactcg gacgcaatta
     4081 tggaaaattg taaatactct tgccggtggc gaacgcgaag ggaagaaacc acgcatcgag
     4141 gtggcgctct acgaatacgg caacagtatg ttgaaagaga gcaatcacta tgtacgccaa
     4201 gttctccctt ttactacgaa tctggatcaa gcttctgaaa aattatttga actaaaaact
     4261 atgggtggtc ttgagtattg cggcgcggcg ctcaacgacg cttttacaaa cctcgaatgg
     4321 aaaaattatg acgatgtgta tcgcgttgtt tttattgcag gcaatgaacc tttcacacaa
     4381 ggccccattc cttttaaggg agcgcttgag aaagcacgcc aaaaaggtat ttttgtgaat
     4441 actatttttg ccggttcgcg ccaacagggt atcgcaacgc aatggctctc tgctgcacgc
     4501 ctcgcctatg gggatttccg tgtaattgat caaaatcaaa tggatcaagt gatggcgacg
     4561 ccttacgacg aagaaattcg taatttgggt gaagcgtata atgctacggt aattcctttg
     4621 ggtgaggtgg gggttattgc acagaaacgg atgcaaacac aagatcgaaa aattgcagca
     4681 actggcgttg aaaacggcgc gctgaccgag cgcgctcaag caaaagcgac agagcagtat
     4741 tctgccgccc atcactggga tttaacgagc atcacacacc ctgataaagc gttagaagaa
     4801 atcaacaaaa atgatttacc acaaacgctg agagaaaaac ctcaagggca aattaaagcg
     4861 tatgttttac aaaaacaagc cgaacgcaaa aaaatccaaa agcggattga tgcacttacc
     4921 caaaaacgga acgtttacat cggcaatgct tcaaagtcaa accgtaacga cgactttggt
     4981 cgagcaacac aagttactat ccgcgcacaa ggaaagaaac gaggttttat tttttgaata
     5041 atttttagaa ttcaaactga taagtcgccg agacatcaaa atacgccagc gaagtttgtt
     5101 tttcattctt gctattgagg tattgataat cggcgcccaa actgggggtt aggtatcctg
     5161 caattttgag gcggatgttc ttaaaccatt tttgcaagct catgctatag cgtaacccga
     5221 caaacgacat cgaacggtcg atggtttgcg tcagcgctgc attcatcggt ctattggttg
     5281 aatcgatgat tgaatacgca ccgccgacgc tcgccactgc aaataccccc aatggcgaaa
     5341 aataataccc cgcacgtacc gccgcttcga cattccacaa gaggcccaca tcgctttcgg
     5401 tggtcgagag ggttggatct atttgcttaa tcgaaaaaat atccatgtat gaatctaaat
     5461 ccaatccaaa cgaaatactt tgtatgttaa atatatgaaa ggaagttttc atataaagtg
     5521 agctactcca gttcaaaagg tcgcgttgaa aaatacctgc ggggtttgtt cctgaaaaat
     5581 tgcgataatt tagcccaacc atatacccct cgttgcgatt accatattcg agaccgatgg
     5641 tttggtttgc gttattctta tagttcgtag gcatagtgtc tttgacatcg gtcgatggcc
     5701 aggtcaagtg cgcatagagt aacaattctt cttgcagccg cgcgtcggcg cgtagataca
     5761 tggcgcttgc ggggtattga taaaagagcg cttgtcgctc gaccgtgttt gatgcgtaac
     5821 ccgcagtaat gttgaggtga cgcttgtcgt tctcgcgtaa ataattgtaa gagtcgtctt
     5881 cttttttggg tgcaggcgcg acgcttttgt tctcattttt tgatagcgct attttttctc
     5941 cttctttagt cggcgtgctc ttttgtgctg tcgcgcgcgc caacgcttcg cgttcaattt
     6001 ctttcacaat ctccgttgct actttattcg tggcgtcaaa gagagtcgcg tcggcgttgt
     6061 tggtcaaagg ggcaatgcta atgaatttat ctggcgcgat atagatgcgt gtgttaaaaa
     6121 cgatttgttt cttgttcggc gtgagcgaat acgaaccgac aacgacataa tcgctttgag
     6181 tggagatggc gagctgttta atttctaaat cggtgggtgt ttggtttttt ttccatagtt
     6241 gttttgtttt tgcatcgacg gctgtttggt ttgcgcggga gtaatcaaat ttctcttgca
     6301 tcgattgatt gaccgcatcg gtgagtgagc ccgatagata gccataattc tttgaggcgg
     6361 tggtgtcgat gtattgcggt atggccacaa cggcaagccc acttttcgcg gcgggttgcg
     6421 gcgctgcaaa aactattgaa attggaataa aaaacgcagc gctatagagg cagcgtaacg
     6481 atacttttat catgtgaagc gcgaacgtat tttctaaaat taccgtgtca actatattga
     6541 tttacgaacc gtgcacaaaa atccgcaaaa aaattgcctc tcgtaaacgc tacgcgagtt
     6601 ggcgcacaac ttccttaccg tacagtttca ttcgtcgcat aatgtcgtgc aatgagatcg
     6661 tcgggatatt cacgatgaat ttcacgaaag attgcacgcg catcgtcgac aaaaccccgc
     6721 cgttgcattt tgagagcttt ttcaaatttt tcttttgtct tatttttacc ttcgcgtata
     6781 tgttccgcgt cgttattaaa aacttcgtag atggtttcct cggtaaagcg cccgcgtact
     6841 ttgaaataac caaccgcacg cgccatcaca cccgagtttt ctggaagctg catcatcgtc
     6901 gtttcagaaa cgagagtatc ggtcggcagc ttgcgcgtga attgctcgag gcgcgccgat
     6961 aaattcacgg ggtcgcccaa gcacgtccac tcgtagcggc tggttgcgcc gacaagaccg
     7021 accatcacac gaccagtggc aataccaaag cctgtgccaa tttgaatttt accaggaagt
     7081 gagatttttt ctatcccttt ttggtattct tgcatttgaa tagcgcattg gattgcttta
     7141 agaggggcat tttttttact cgcaccgaga ccaaagacaa ccatgatgcc atcgcccaaa
     7201 aatttatcga taatgccacc gtgttgttca acgatttcaa ctacgccttc gtagtagaca
     7261 ttcaacgcca tataaagcgt gtttgcaggg agtacctcag cgatacttga aaatccacgg
     7321 atgtccgaaa aaagtatcgt gagttcttca aaactcactg cgtgatctgc cgccaaaagg
     7381 cggttgattt catttagctt ttcaccttct aattcctgtg cgagcttttt ttgggaggct
     7441 actttttctc gtaactcgct gcgttcaaga agcaaaaagc cgatagcgca aagcgataaa
     7501 aaaatgacac tttgccacac tactaaaagc tttaagtcga ggctgcgata ttttaaatca
     7561 aacgcgatta aaaaaacaag catagcgatt atcgataatt gcacgaccaa tgcgtgtaat
     7621 agcccattcc aaaaaacagt tgcgataatg atacaccaaa gcaccccgta gaggaagtag
     7681 ggtgttaatc gaaactgggc ggaagggaaa agaaaaacga gcgataaccc caccaacaga
     7741 aatagcgcca agcgaatgac gcggtttcta taactgtggg aggttgaact catcgaggag
     7801 atccttaagt agcaacgcta agcgcgatgc ttcgtgtgcc atttgcgtta aatccatcgc
     7861 ggtggctgcc gtcgaggacg cttcggtcga taaaatgctg atggttttac taatctcacc
     7921 gctggcgcta ttttgtgctt cggcttcttc aacaatttgg tcgtgtagag ttgtgagttt
     7981 tccaatatct ttttcatttt catcgatgga ttgagcaaat tcgtccaact ggtttcgagt
     8041 ttgcatatat gaatcactcg cttttatgtg catgagaagt gtacgtttaa gaagaacagc
     8101 cacaccgctt aaatcggcta taagcttctg caacgttttt atttctgtta gattgttgtt
     8161 ttgtccttct tttatttttt catcaaaaat tgtttcagca ttcccaatag catcgtttag
     8221 atttttataa acaaccttga agtcgtgcgt attctcttca taagcatctc gaaacgaagc
     8281 tgttttaccc gaaagcttgt ggtacatatc gccccacgca accgcgtatt cgacggtgcg
     8341 gcgcactagg ctatggaaat cttttagctc gtctttttcg gttgaaagcg aagtgttcaa
     8401 atcttcgacg gcttgcgatg tttcttcgat gacgtttgct tgcctctcgg agaggcgcaa
     8461 gaaattttct gcggtttcgc gcgtcttgtt tgctaaagat gaaagttgat ggctaatttt
     8521 tcccagcttt gtcaaaagtt caaccgagta ttcgatgaat gtgttaacgc cgtcgccgac
     8581 gcggccaatt tcatctctat aacgtggatt tatttttata gtgtagtctt tttgttgcaa
     8641 acgctgaaac gataacagca cttgctccaa tggagttgaa ataataaacc gtgacaccaa
     8701 taaaatacct ataataagaa tggcgacaac gacaagaccc acaccggcaa tttcaagaga
     8761 gaggcggcgc gattgttttg taatctcgac taaggaaacg cgcatggtga ttgctcccaa
     8821 cacttcgttt ggttttactt gatggcatgc gaggcaattt ttgccgagat aattggcttg
     8881 cgcatggatg ggttttacaa tccgcaagta ttcatcattt tcattttctg tatgaataac
     8941 tccggtgcgt atcactgcgt tttcgtattc gttttttgct ttttcacttt cacgccccgc
     9001 gccgtatgct gcaataactt taggtccact aataatacgc aagtcgctta caatgcccgt
     9061 ttcgttgatt tgactgagca gttgctctcg gtgctccatt tgtccggtaa tcatgagcgt
     9121 tgtgagtcca ctcaacgcca tcttctctgt gctgtcggca aaaagatgcg cttcccgtac
     9181 cgccgatttt ttttgcatgc ttcggatgct cacactggca aatgcaatgg caatcaaaaa
     9241 gatggaggtt gttactccga tgaactttgt cgcaatcgat gcgttcaaga gacgttctat
     9301 aaaactcttc acaaaaactc cccaaagtta attttcttgg gtggtaccta aaaaatcacg
     9361 gttataaagc gaaagagggc atctgagacc gctcggcgtt cgatttacaa agtaaatcgt
     9421 agcgttttct ggttataggt gatttttcga cactccctca atagcaaacc ttgacttagg
     9481 acaagactat ggtcgagata gcgcgtcagt gacttattat gagcctgtgc gcattttgat
     9541 tatagaatac aaaaaatgct tactttgctg agtccatttt aaggagagtc gatttatatg
     9601 caacataaca attataaacc tgctacgtta gccgttcatg gaggcgaaag gacctacgac
     9661 ggctcgccgc acacgccagt gtttaacacc acgacatttg cctttgaaaa caccgctgct
     9721 ttgctcgacg tagtcgatgg aagaaaaaca ggtccgctct atacacgcta cggtcttaat
     9781 ccatctataa ccagcctcga ag
//
LOCUS       DAOMPD010000028         9733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3012_length_9733_cov_3.359888, whole genome shotgun sequence.
ACCESSION   DAOMPD010000028 DAOMPD010000000
VERSION     DAOMPD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3012_length_9733_cov_3.359888"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1003
                     /locus_tag="PLY93_01825"
                     /pseudo
     CDS             28..1003
                     /locus_tag="PLY93_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
     gene            1020..2795
                     /locus_tag="PLY93_01830"
     CDS             1020..2795
                     /locus_tag="PLY93_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP68253.1"
                     /translation="MRFFVGIVDTIIDTGTGFTRDEKTRKLVRILNWALTIGVLIAAV
                     QAIAFFRFDFNGPTFGSLFFIVGCFLCLLLQYLGFIRLAPVLFIFIAVAVIAYQHLFF
                     GFKAGFWLILLNLTQATFALFPFLKIRYHFILAGFYVTSLIVLVMLTMSQPPLYNGVT
                     QELSDTYFRVNLIRSSILFVLLAYYLVYESLRSEKEVKFTAQKAMEAAEAKSFFLSNI
                     SHELRTPMNAIKGFSDILLDATNSITDEKQRLEFRTRLQQIEISAANLMTIINDMLDF
                     TRIEMRTISLHKKDFDLFRLCENIMQTAQFYGHKNTGIKTELFFAPRLPRFVYGDSTR
                     VSQILINLLSNAMKFTHQGTIALRVMLSEQRDNGYTICFEIEDTGIGIPSEKLTRMFE
                     RFSPVSRETAMRYGGTGLGLAISKQLVELQGGEISVMSSPGVGTLFLVNLPFEKAREN
                     YSKPAAGKYDLQQKRILVAEDNEVNQLLVKTLLESWNAEVTIVDNGLKALGSVTKRDY
                     DLILMDLQMPFIDGFEATEQIRALENKEKKITPIIALTADVLSETRQKVFAVGMNNIV
                     TKPINQAELFAAISQVLMTDLPKDI"
     gene            complement(2752..3792)
                     /locus_tag="PLY93_01835"
     CDS             complement(2752..3792)
                     /locus_tag="PLY93_01835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRP68254.1"
                     /translation="MKNILVIRLSAIGDILLTTPVLVRLKKLYPDVKIHFLVKEQYAS
                     FVEACPEVDSIILWKKNESLFSIRKQILATSILFDLVVDLQSNLKSRILTKLLRKKIS
                     RRYVKPYWPRLLLVYFKINRYEEIKKTTERYLDAIHDLTDAPLTETISLKRKKLPAAV
                     QRFIKKNTILIAPGARWFTKRWPQEYFASLASKILEKSPSDKIIWLGGPDELELFEFF
                     KKHPYLLRHTKRMLFLSNALSVEEMATLADSVQVMVCNDSGLMHLLSTSSTPLVGLFL
                     STVEEFGFFPLAQQGEVLMARNIECRPCNHKGLPACPKKHFRCARELTADQVYSMVLK
                     YLWANPSSIPAK"
     gene            complement(3789..4430)
                     /gene="tmk"
                     /locus_tag="PLY93_01840"
     CDS             complement(3789..4430)
                     /gene="tmk"
                     /locus_tag="PLY93_01840"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRP68255.1"
                     /translation="MKGLFVVFEGIDGSGKTTAIERIAQILQEQNFQTIVLREPTEKT
                     DASREIRRILKTVPTMDERIERELFDLFLIDRLWDIEHQIKPALSSGGIVLLDRYFIS
                     TAAYQANSSEKVRQIVMDYLDDTRILLPDIIFFLDIPVKTAIDRLSGRNSLDVFESEE
                     RLTAIQHRYNGALNILAEKQSGVKIERLSHALADKDFLSMSAKIAKAHTEGNS"
     gene            complement(4427..5317)
                     /locus_tag="PLY93_01845"
     CDS             complement(4427..5317)
                     /locus_tag="PLY93_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRP68256.1"
                     /translation="MAETQRHFEKTLTPLLRFKTLDALRDGIFTPIERGITPSGIATP
                     AALIEQIGRRIQDDIPFFKTIHNMFQVNPARQWEGDAALFYVHPTAQISQYAVLNTTE
                     GPIVIDAQARVSAFSFIKGPAYIGKKSQLDQCNFSNSICGTNCRLGGEIADSIIGDYS
                     NKHHEGFLGHSLVGDWVNLGALTTTSDLKNNYGEVRLRYKEEIFPTGTIKFGSILGDY
                     TKTAIGTLLSTGTIVDAGANLFGFPMVSGYIAPLRWGMHETYDVEKFLRDAARMMQRR
                     GASVTPTLEEAIRRMVKGIP"
     gene            complement(5324..6109)
                     /locus_tag="PLY93_01850"
     CDS             complement(5324..6109)
                     /locus_tag="PLY93_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68257.1"
                     /translation="MAETKKLFSKENKAGDKFNYTRKLFHLVGLIIPFGYFFNILDGI
                     SPWQFKETTRSLIFIITGSITIIFLIVEFLRFRFKFWQDLFLKIAGPMLKGKEADKMH
                     GSVPYMLALCLVVGFFPREVAILSVLFLTFGDPAAALIGGRFGTIRFYNGKSLQGTLG
                     GIAGAFLAGLLFLLLISIAQIQSPYLLWDMHGIRLENWVMLFMGAIVALLVEFISHEG
                     FFDDNLTIPVASSLTMIGVHTFLTSANITTYFYSFNDLIRLIS"
     gene            complement(6141..6494)
                     /locus_tag="PLY93_01855"
     CDS             complement(6141..6494)
                     /locus_tag="PLY93_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP68258.1"
                     /translation="MPAPAEQQFNVAEEHAQTYIFFRVTGAMNSQNTHEVRRKFLDAA
                     AKNHVLLDLSGVDLIVSTGIGLLFEMSEQLARNGKKLVLIQPSARVQQVISMTGFTDM
                     FSYAQSRVTAEKVIN"
     gene            complement(6542..8077)
                     /gene="guaB"
                     /locus_tag="PLY93_01860"
     CDS             complement(6542..8077)
                     /gene="guaB"
                     /locus_tag="PLY93_01860"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRP68259.1"
                     /translation="MARGTSGKSSSSFLDGFSAQAVFGKDQGITYKDFLILPGYIDFA
                     PQDVSLETRISRNIKVKLPIISSPMDTVTESQMAITMALLGGIGIIHNNLSIEDQAAQ
                     VEKVKRFENGFITDPIVLSPKNTIADVDAIAARYGFHGIPITEDGTLRTKVVGIVANR
                     DIDFEKNRKLPLSQVMTTELVTAKKGISLVEANEILRKSKKGKLPIIDKEGRLVALMS
                     RTDLKKNHEYPFSTKDEQKRLRVGAAVSTHPHDRERIDMLVARGVDILVVDSAQGYSK
                     FQIDLLKELKKKYPEVDVMAGNVVTREQGEALIKAGADALRIGMGPGCICITQDTMAC
                     GRAQATAVYYTALMAAKHKIPVVADGGIVNIGDIAKALAVGASSVMCGSLLAGSKEAP
                     GDYFYENGVRLKKFRGMASIEAMEGSGGGAKRYNVEDQKIRVAQGVSGAVVDKGSLLE
                     FVPYLMQGVRHALQDMGCRNVFELHHALYSEKLRFEPRSLAAQAQGGVHGLYSYKKPI
                     IGD"
     gene            complement(8114..9331)
                     /locus_tag="PLY93_01865"
     CDS             complement(8114..9331)
                     /locus_tag="PLY93_01865"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HRP68260.1"
                     /translation="MSADTTQVKCPECGTSIDVQDILKHQLEDELKKKYETRHAQAEK
                     KLEEEKEKLAQEKSELEKRINEEVKSKAKALEKEIQTKLTQEQSEQLESLQKELNEKS
                     EKIKELNRSKAEIEKLKREKEELKEEIEAESEKKLNEALIAEKEKIKKLEEQKTELRI
                     KELQKQLEDQKRLTEEMKRKQEQGSMQLQGEVQELAIEEWLKNKFPLDTIDEIKKGSK
                     GGDCLQTVNTDIRSGCGKIYYESKRTKEFQPNWIEKFKNDMREKEADIGVIVTEAMPK
                     ELERMGKISGVWICTYEEFKSLCFVLRESLIQISAALSSQENKGDKMTILFNFLTGNT
                     FRAQVEAIIEGFTAMQTDLTREKNAMQRIWKEREKQIEKVVYNTIQMHASIRGIAGNA
                     ILPISALELPDGG"
     gene            complement(9328..>9733)
                     /locus_tag="PLY93_01870"
     CDS             complement(9328..>9733)
                     /locus_tag="PLY93_01870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRP68261.1"
                     /translation="RIKAGDVVVIKNEGPKGAPGMPEMLKPTSALIGAGLGNSVALIT
                     DGRFSGGTHGFVVGHITPEAYEGGVIALVEDNDIIELDADKNTITLKVSDAVIEERRK
                     KLPPPKLKVSSGVLFKYAKQVKSAAEGCVTDE"
BASE COUNT         2453 a         2245 c         2205 g         2830 t
ORIGIN      
        1 tttgaatttg ccaccgtggg tggctgggtg tcggcgcgcg gcgcaggcca agcatccact
       61 ggttacggac gcatcgagga catcttagtg ggattaacac tcgtaacgcc ttccggcaaa
      121 attgaatgcg gcaattaccc tgcggcgtcg gtgggaccgg acatccgcca ttttgtttta
      181 ggtagcgaag gtacgttggg tgtaattaca aaagtgacgc tccgcgtgcg gcgttacaac
      241 gccgacaaca tggtgatgaa atcgtttatg tttaaatcgt ttgaaggcgc agtgacggcg
      301 atgcgcacca tgatgcaaag cgaggtatat ccccctcatt tttttcgtat ctccgacccc
      361 gaagaaacag acggcgcgct cgctatgaaa gccaaagaca aaggtctcac cggatgggcg
      421 ttgaaagctc tgggtttcaa agagaattcc cgctcgctta tgtatgctat ttttgaaggc
      481 gacaaagacc aagcaaaact cggtgcaaaa aaattgagtc gcatcgctaa aaaatgtggc
      541 ggaatgtcgc taggttctta cgccactgaa aaatggttgg aacaacggta ttcatccgcg
      601 tacatgcgcg accccatgat ggacgctggc atccgtatcg acacgctcga aacgtcggtg
      661 cattattcgc gtctttttga actgtggaaa gcggtgcgtg catacatcaa acaagacgga
      721 caaacgatgt gcctcacaca catttcacac acgtacgaaa ccggtgcaaa cttgtatttt
      781 atttttgcgt cgcccatgct tgccaaagac gaattgactc gctttgaaaa atttcataag
      841 ggcatcatcg acacttttct tgcacacggc ggcacgttat cacaccacca cggcgtgggt
      901 cgtcttgccg caacactcac ccacaactct cgcagcatgg cgcgcggtca aaaaagcgct
      961 cgatcccaaa gggattatga atccgggcgc gcttattttt tgatgtcatg tcagccgtag
     1021 tgcgtttttt tgtaggtatc gtggatacca ttattgatac cggtaccggt tttacccgtg
     1081 atgaaaaaac acgcaagttg gttcgtatcc ttaattgggc tttaactatt ggcgttttga
     1141 ttgctgcggt acaagcgatc gctttttttc gattcgactt taatggacct acattcggct
     1201 cgctattttt tattgtgggt tgttttttat gcttgttact tcagtacttg ggttttatac
     1261 gattagctcc ggttcttttt atctttatag cagtagcggt aatcgcatac caacatttat
     1321 tctttggctt caaagcgggg ttttggttaa ttcttttgaa tttaacacaa gcgacatttg
     1381 cattattccc gtttctaaaa atccgttatc attttattct tgctggtttc tatgttacat
     1441 cactgattgt tctggtgatg cttactatgt cgcaacctcc cttgtataac ggagtaacgc
     1501 aggagttgtc ggatacgtat tttcgtgtaa atttaatccg tagttcaatt ctcttcgttt
     1561 tactcgctta ctatcttgtg tacgaaagtc tacgctctga aaaagaagta aaatttactg
     1621 cacaaaaggc catggaagcg gcagaggcaa aatcgttttt tctctccaac atttcgcacg
     1681 agttacgcac tcccatgaat gccattaaag gattttcaga tattctcctt gatgccacaa
     1741 attcaatcac cgacgaaaag cagcgtctcg agtttcgcac gcgcttgcag caaattgaaa
     1801 tttctgcggc aaatttgatg acgattatca acgacatgct cgattttacg cgtatcgaaa
     1861 tgcgtacgat ttctttacac aaaaaggatt ttgatctttt tcggttgtgt gaaaatataa
     1921 tgcagacggc acaattttat gggcataaaa acacaggtat aaaaaccgaa ttattttttg
     1981 cgcctcgttt accccgtttt gtctatggcg attcaaccag agtgagtcaa attttgataa
     2041 acttgttgag taacgcgatg aaattcacac atcaaggcac gattgcttta cgtgtaatgc
     2101 tttccgagca acgcgacaat ggatacacaa tttgttttga aattgaagat acaggcatcg
     2161 gcataccttc agaaaaattg actcgtatgt ttgagcgttt ctcccccgtg tcgcgtgaga
     2221 ccgctatgcg ctacggcggc acaggtttag ggttagcgat tagcaaacaa cttgtcgaac
     2281 tgcaaggtgg ggaaatatcg gtgatgagca gtcctggcgt aggtacactt ttcttggtaa
     2341 atttaccgtt tgagaaagcg cgtgaaaatt actcaaagcc agcggcaggt aagtacgact
     2401 tacaacaaaa acgaatcctt gtcgccgaag acaacgaagt caaccaactt ttagtgaaaa
     2461 cccttttgga atcatggaac gccgaggtaa ctattgtcga taatggtctt aaagctctcg
     2521 gttctgtcac aaagcgcgat tacgatttaa ttctcatgga tttacaaatg ccttttatag
     2581 acggctttga ggcaacggaa caaatacgcg ctttagaaaa taaggaaaaa aagataaccc
     2641 ccattattgc attaaccgcc gatgtgttga gcgaaacgcg tcaaaaggtt tttgccgtag
     2701 ggatgaacaa catcgtcacc aaaccaatca accaagcgga actttttgcg gctatttcgc
     2761 aggtattgat gacggatttg cccaaagata tttgagtacc atagaataca cttgatcggc
     2821 agtgagttca cgcgcgcatc gaaaatgctt tttggggcat gcgggtagtc ctttgtgatt
     2881 gcagggacgg cactcgatgt tgcgcgccat caaaacttcg ccttgctgcg ctaaaggaaa
     2941 aaagccgaac tcttcgacgg tgcttaaaaa aagtccaaca agcggcgttg acgaagttga
     3001 aagtaaatgc atcagcccgc tatcgttgca caccatcact tgcacgctat cggcgagcgt
     3061 tgccatttct tctacactca atgcattcga gagaaaaagc attcgttttg tatgacgcaa
     3121 aagataaggg tgtttcttga aaaactcaaa aagttcaagc tcgtcggggc cgcccaacca
     3181 aataattttg tcgctggggc ttttctctaa aattttggaa gcaagtgaag caaagtattc
     3241 ctgcggccaa cgcttggtaa accaccgcgc acctggggca atgagaatag tattcttttt
     3301 gatgaatcgt tgaactgccg ccggtaattt tttgcgcttg agagaaattg tttctgttag
     3361 gggcgcgtcg gttaaatcgt ggatggcgtc gagataacgc tcggttgttt tttttatttc
     3421 ttcgtaacga ttaatcttga agtaaaccaa taataagcgc ggccaatacg gtttaacata
     3481 acggcggctt attttttttc ggagcaattt tgttaaaata cgcgatttga gattgctttg
     3541 taaatcgacg actaaatcaa aaagaatact ggttgccaaa atttgttttc ttatagaaaa
     3601 taacgattca ttttttttcc ataagataat tgaatccact tcaggacatg cttcaacaaa
     3661 agaggcgtat tgttctttga ctaaaaaatg aattttaacg tcagggtata attttttaag
     3721 gcgaaccaac accggcgtcg ttaaaagaat gtcgccgatg gcgctgaggc gaatgacgag
     3781 tatgtttttc atgagttgcc ttctgtgtgt gcctttgcaa tttttgcgct cattgataga
     3841 aagtctttgt cagcgagggc atgagataga cgctctattt tcactccgct ttgtttttct
     3901 gctaagatat taagcgcgcc gttatagcgg tgttgaatcg ccgtaagtct ctcttcgctt
     3961 tcaaacacgt ctaatgaatt gcgtccactg agccggtcga tagcagtctt tacgggaatg
     4021 tcaagaaaaa aaataatatc gggtaaaaga atacgggtat cgtcgaggta atccatcaca
     4081 atctgccgca ccttttcgct actatttgct tggtacgcag cggtggaaat aaaatagcgg
     4141 tcaagcaaaa cgatgccacc gctgctaagc gcaggtttaa tctggtgttc gatgtcccac
     4201 agacgatcga tgaggaataa gtcgaatagt tcgcgttcga tacgctcgtc catcgtgggt
     4261 actgttttca aaatacggcg gatttcccgc gacgcatcgg ttttttctgt gggttcgcgc
     4321 aagactatag tctgaaaatt ttgttcttgt aaaatctgcg cgattctttc gatagctgtt
     4381 gttttgccgc tgccgtcaat accttcaaaa acgacgaaca accctttcat ggaatccctt
     4441 ttaccatgcg gcggattgct tcttctaacg tcggtgtcac cgacgcgcca cgccgttgca
     4501 tcatacgcgc ggcgtcgcgg agaaattttt ccacatcgta agtttcgtgc ataccccatc
     4561 taagcggtgc gatataaccg gacaccatgg ggaatccgaa taggtttgcc cccgcgtcga
     4621 cgatggtgcc ggtggaaagt aaagtgccga tggctgtctt tgtgtaatca cctaaaatag
     4681 agccaaattt aatcgtgccg gttggaaaaa tttcttcttt ataacgcaat cgcacctcgc
     4741 cgtaattatt ttttaaatcc gacgttgtgg tgagcgcgcc taaatttacc caatcgccca
     4801 cgagcgaatg tcccaaaaat ccttcgtggt gtttattcga gtaatcgcca atgatcgaat
     4861 cggcaatctc gccacccaaa cggcaattgg tgccgcaaat ggaattcgaa aaattacatt
     4921 ggtcgagttg ggatttcttc ccgatgtacg ctggaccttt gataaaagaa aatgccgaca
     4981 cgcgcgcctg tgcgtcgatg acgatgggac cttctgtagt gttgagcacc gcgtactgtg
     5041 aaatttgagc ggtgggatgg acataaaaca acgcggcatc cccttcccat tgccgcgcgg
     5101 gatttacttg aaacatgtta tggatcgttt tgaaaaaagg aatgtcgtct tgaatacggc
     5161 gaccaatctg ctcgatgagc gcagcggggg tagctatccc actgggcgtt ataccccgtt
     5221 caatcggcgt gaaaattccg tcgcgcagcg catcgagcgt tttgaaccgt aaaagcggtg
     5281 taagtgtttt ttcaaaatgc cgttgcgttt cggccataac gattcacgaa ataagacgga
     5341 ttaaatcgtt aaacgaataa aaatacgtcg ttatgttagc gctcgttaaa aacgtatgca
     5401 cgccaatcat cgtcaaagac gatgcaacgg ggattgttaa attgtcgtca aaaaaacctt
     5461 cgtggctgat gaactccacc aagagcgcca caatagcacc cataaaaagc atcacccagt
     5521 tttctaaacg gatgccgtgc atatcccaca gaagatacgg ggattgaatt tgtgcgatag
     5581 aaatgagcag tagaaaaaga agtcctgcaa gaaacgctcc ggcgatcccc cccaacgtgc
     5641 cttgtaaact tttaccgttg taaaagcgga tggtgccaaa gcgaccgccg atcaaagccg
     5701 ccgccggatc gccaaacgtc aaaaaaagca ccgaaagaat cgccacctcg cgtgggaaaa
     5761 aacccaccac taaacagagc gccagcatgt aaggcacgct gccgtgcatt ttgtcagcct
     5821 ctttaccttt gagcatggga cctgcgatct ttaaaaaaag gtcttgccaa aacttgaaac
     5881 gaaaacgtaa aaattcgaca attaagaaaa taatcgtgat actgcctgta ataataaaaa
     5941 tgagcgaacg cgtcgtctct ttgaattgcc acgggctgat accgtcgaga atattaaaaa
     6001 aatagccaaa ggggatgata agccccacaa gatggaaaag tttgcgtgtg tagttgaatt
     6061 tgtcgcccgc tttattttct ttactaaata gttttttcgt ttctgccatg aaataccgtc
     6121 gtttgttgtg gagataaaac ttaattaatg actttttcgg cggttacacg cgattgcgca
     6181 tacgaaaaca tatcggtgaa accggtcatg ctaatcactt gctgcacacg cgccgaaggt
     6241 tgaatgagca ccaatttttt tccattgcgt gccagttgct cgctcatctc aaacaaaaga
     6301 ccgatacccg ttgaaacaat gagatcaaca ccacttaaat cgagaagcac atggttttta
     6361 gcggcggcat cgagaaattt acgccgtact tcatgcgtgt tttgcgagtt cattgctcct
     6421 gtaacacgaa agaaaatata cgtttgtgcg tgttcttcgg cgacattaaa ttgttgttcg
     6481 gcaggcgctg gcattgattt ggcaatctac tacgtcaagg cgtggtagca agtaaaaatt
     6541 actaatcccc aataattggt tttttgtaag aatataaacc gtgcacgcca ccttgcgctt
     6601 gcgccgccaa cgagcggggt tcaaagcgta gtttttcaga gtagagcgcg tggtggagct
     6661 caaatacatt gcgacaaccc atgtcttgga gtgcatggcg cacgccttgc atgagatacg
     6721 gcacaaattc gagaagacta cctttatcga ccaccgcacc agaaacgcct tgcgccacac
     6781 gaattttttg atcttcgacg ttgtagcgtt ttgcgccgcc gcccgagcct tccatcgctt
     6841 caatcgaagc catgccacgg aattttttaa ggcgtacgcc gttttcataa aaataatcac
     6901 ccggggcttc tttgctgccc gctaaaagcg aaccgcacat caccgaactc gcacccaccg
     6961 caagcgcttt agcaatgtcg ccgatgttga caatgccacc atcggccacg acggggattt
     7021 tatgtttcgc cgccatgagc gcagtgtagt aaacggcggt cgcctgtgcg cggccacacg
     7081 ccatggtgtc ttgagtaata caaatacaac ctggccccat gccaatgcgt agtgcatccg
     7141 cgcccgcctt aatgagcgcc tcgccttgtt cgcgcgtcac cacattgccc gccatcacgt
     7201 ctacttctgg gtatttcttt ttgagctctt tgagtaaatc aatttggaat tttgagtaac
     7261 cttgcgccga atcgacaaca aggatgtcca caccacgcgc gaccagcatg tcgatgcgtt
     7321 cgcggtcgtg agggtgtgtc gatactgccg ctcccacacg tagacgtttt tgttcgtctt
     7381 tggtggagaa cgggtactca tgattttttt taagatcggt gcggctcatg agtgcaacaa
     7441 gacggccttc cttatcgatt atgggcagct tgcctttttt tgatttccgt aaaatttcat
     7501 tggcttcaac aagggaaatc ccttttttcg cagtcacaag ttcggttgtc atcacttgcg
     7561 ataaagggag ttttcgattt ttctcaaaat caatgtcgcg gttggcaaca atccctacaa
     7621 ctttggtgcg cagcgtgcca tcttcagtga tggggatacc atggaagccg taacgcgcgg
     7681 cgatggcgtc gacatcggca atggtgtttt tgggggaaag cacaatcggg tcggtgataa
     7741 aaccattttc aaaacgcttt accttttcca cttgcgccgc ttggtcttct atcgaaagat
     7801 tattgtggat gatgccgata cctcccaaga gcgccatcgt aattgccatt tggctttcgg
     7861 taacggtgtc cattggcgag ctgataatgg gaagctttac tttaatgttg cgggaaatgc
     7921 gggtttcaag gctgacgtct tgcggagcaa aatcaatata cccgggtaag attaaaaaat
     7981 ctttgtaggt gatgccttgg tctttaccga aaactgcttg tgctgaaaaa ccgtctaaaa
     8041 aggaagatga acttttaccc gatgtaccgc gcgccatgtg ataggttagg gagaaaattc
     8101 acgttgtcaa gctctatcca ccgtccggca actctaacgc tgaaatcggt agtatagcgt
     8161 tacccgcaat ccctcggatg gaagcgtgca tctggatggt attatacacc actttctcaa
     8221 tttgtttttc gcgctctttc caaatccgct gcattgcatt tttttcacgc gtgagatccg
     8281 tttgcattgc ggtaaaacct tcgataattg cttcaacttg cgcacgaaac gtattgccgg
     8341 ttaaaaaatt aaaaaggatc gtcattttat cgcccttgtt ttcttgcgag ctgagcgcgg
     8401 cgctaatttg gatgagcgat tcacgcaaca caaagcaaag actcttgaat tcttcgtacg
     8461 tgcaaatcca aacgccgcta atttttccca tccgttccaa ctctttcggc atcgcttcgg
     8521 tgacaatcac ccctatatcg gcttcttttt cgcgcatgtc gtttttgaat ttttcaatcc
     8581 agttgggttg aaactctttc gtgcgtttac tttcgtagta aatttttcca cacccactgc
     8641 ggatgtcggt gtttaccgtt tgcaggcaat cgccaccctt cgagcctttt ttgatttcat
     8701 cgatggtgtc caaggggaat ttatttttga gccattcttc gatggctaac tcttgcacct
     8761 cgccttggag ttgcatagag ccttgttctt gcttgcgctt catttcttcg gtaagccttt
     8821 tttgatcttc gagttgtttt tgcaattctt tgatgcgcag ctctgttttc tgttcttcga
     8881 gttttttgat tttttctttc tcggcgatca acgcttcgtt gagctttttt tccgattcgg
     8941 cttcgatttc ttctttgagc tcttctttct cgcgtttgag cttttcaatt tccgctttgc
     9001 tgcggtttaa ctcttttatt ttttccgatt tttcgttgag ttctttttgc agcgattcta
     9061 attgttcgct ttgttcttgc gttaactttg tttgaatttc tttttctaac gctttcgctt
     9121 tggattttac ctcttcgtta atccgttttt ctaactcact tttctcttga gccaactttt
     9181 ctttttcttc ttctaatttt ttttctgcct gggcgtgcct cgtttcatat ttctttttta
     9241 actcgtcttc taactgatgc ttcaatatat cttgcacatc aatcgatgtg ccgcactcgg
     9301 ggcatttgac ttgtgttgta tctgcactca ttcgtcggtc acgcagccct ccgctgcgct
     9361 tttcacttgt ttcgcgtact taaataaaac acccgagctg actttaagtt tgggcggggg
     9421 tagtttttta cggcgctctt cgatgactgc atcgctcact ttgagcgtga tggtattttt
     9481 atccgcatcg agttcaataa tatcgttgtc ttcaacaagc gcgatgacac cgccctcgta
     9541 cgcttcggga gtaatgtgcc ccacaacaaa accgtgcgtg ccgccgctaa agcggccatc
     9601 agtgatgagc gcaacgctgt tgcctaaccc tgcgccgatt aacgccgagg tgggtttgag
     9661 catctctggc atgcccggcg caccttttgg cccttcgttt ttaatcacaa ccacgtcgcc
     9721 cgctttgatg cgg
//
LOCUS       DAOMPD010000029         9637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3051_length_9637_cov_3.042997, whole genome shotgun sequence.
ACCESSION   DAOMPD010000029 DAOMPD010000000
VERSION     DAOMPD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_9637_cov_3.042997"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1325
                     /locus_tag="PLY93_01875"
     CDS             120..1325
                     /locus_tag="PLY93_01875"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRP68262.1"
                     /translation="MRYFICCILAFTFVNTVDAKKHFKVRPGLDTLMQKSDKWLKGKR
                     IALIVNHSAVDSKGRHILDLLYPQYKVVKIFAPEHGVRGALDEHVDDEKDTKTGIPIV
                     SLYQRKKKAPTNEDLADVDVLIFDIQEIGLRYYTYASTMVMAMKAAKSAGKTMYILDR
                     PNMAGPLGVYGPLLEKKFHGGFAGYYPIPIAHGMTMGELALYYNKTFDIGANIRVMKM
                     ANYRRGMYYDETGLPWKNPSPSIVRMASVIGYHLAGAFETLNISVGRGTDHPFEVYGA
                     PFFDAQKITRELTQAKLPGLKFTPTTFTPTRSAFKGKACHGFKLAITNRRKIQPMRTV
                     ITIGRILYKNLPEETRKANWARVANGIGDTAFIERIAKGENYKKLLKLLKKEVHGFIK
                     SRKPYLLYR"
     gene            complement(1358..2533)
                     /locus_tag="PLY93_01880"
     CDS             complement(1358..2533)
                     /locus_tag="PLY93_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68263.1"
                     /translation="MGLAPGGFMKFQKLQVNENAQRVNDTLEAYVNFAVRGFDTLMGT
                     VGLGGRAADYDDLNYEFMGGANDELRKKHYDKSLRLLWKAEKHAEWSTFNDLNKEEAM
                     LLKMAGESLNQGEKKQLERMKSAEFKELLAREYTPAQKQALVNILSMIGHGEAYAWLV
                     SNEVLRDVKSTGAKAALTMQVMEEAKHFVVLRELIQAFEMPIPRLTGYEYLILEGTLK
                     SKGLEKFFGMNVLVEGIALSIFGALSKFPGLEILEMFHLDESRHCALPTNYLKDRPLT
                     WWERNNPAAMLKRFSLVLPAIPLAFKMEKDFAELGIDVFDFGGSLVRKVLNLAYRVGF
                     HLPMPQTMMKDFINMLFNAYCMATRDGHTFQAYLDREATMGEKERAVEQEVFMMASA"
     gene            complement(2642..3274)
                     /locus_tag="PLY93_01885"
     CDS             complement(2642..3274)
                     /locus_tag="PLY93_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68264.1"
                     /translation="MKLLSLTVFFALVFSACNNYGLLESLQNPGAISNTPPPVKRIFV
                     TTTLSNGNMSNFSCNGVGIQNANCLCQQTAQQYGFGGTYIAFLSDDLADMSCRLFSVA
                     SSACSMTSDAVWYNTKNEIVASGTSELFSIAHTAAIYDERGDAPMGAVWTGTTATGAL
                     ASSHCNRWTSNSSGVQGQTGTPSATTGDWTEGGAAACNTNQSFYCIAVVE"
     gene            complement(3376..3447)
                     /locus_tag="PLY93_01890"
     tRNA            complement(3376..3447)
                     /locus_tag="PLY93_01890"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3412..3414),aa:Glu,seq:ttc)
     gene            complement(3496..3568)
                     /locus_tag="PLY93_01895"
     tRNA            complement(3496..3568)
                     /locus_tag="PLY93_01895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3533..3535),aa:Lys,seq:ttt)
     gene            3767..5133
                     /locus_tag="PLY93_01900"
                     /pseudo
     CDS             3767..5133
                     /locus_tag="PLY93_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803368.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
     gene            5130..5735
                     /locus_tag="PLY93_01905"
     CDS             5130..5735
                     /locus_tag="PLY93_01905"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRP68265.1"
                     /translation="MKTLIIDNYDSFTYNLYQYVGEVLEEKNKVFTVDVFRNDEKSYE
                     EIKSAAYDRIIISPGPGHPADAKYFGVCTHILENATKDTHFKILGVCLGMQGMAHIFG
                     GKVVKAHQPMHGKTSPIKHDAKGVFAGLPQGIEVMRYHSLIAEEASLPPSLEITARTA
                     DGSHEIMGLRHKTLPIEGIQFHPESFATEGGKVMLRNFLSA"
     gene            complement(5737..6345)
                     /locus_tag="PLY93_01910"
     CDS             complement(5737..6345)
                     /locus_tag="PLY93_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68266.1"
                     /translation="MSSEKQMTRCASCGGTEFELLMSGKYKCKHCGSQQLPSAIQTYV
                     VDAKTPFFEGNIFKKYPKTLIAGGVGFAFLVYSLISFINARSALSDLTNAYNEVMLQR
                     QRTTDILQRNKIVLDQQYQIVFEKIPLEKIIAKLTIANDSVSQRILDELSGGYNREVV
                     ALRRYKKAQEEWRGQRNWLTAWMGGKYILKDAQDNRELIQRK"
     gene            complement(6361..7842)
                     /locus_tag="PLY93_01915"
     CDS             complement(6361..7842)
                     /locus_tag="PLY93_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRP68267.1"
                     /translation="MKKILPVLCFLSLSLHALPIEDAIQQIKNPRSSNAWITYDASVM
                     TAQQVGALNQLLEKINQKTGIEFAIIFKQNLPVESAKDYAHQIFNTWKIGNRETNTGL
                     LLVVLVNQRQWAFEVGYGLETALPDSFTGSLGRNTMAPEFRRGDYYKGIADAINEIEK
                     KIDATPKEELGAKAPFQLEIGKATYNLRDTVFIFAHIAIFLVVVFFILQTASKPNSLE
                     KAKTIHLFFMQLFVAAIGIILSFFYTSWFFKSFFLASEIIAMVIIHKNWNSITKAPVS
                     LKRFYVFLFILVGIAAVVPVFFFWRSPSEYYAAIPFLILAILIFPIYFWTRQAIRRGK
                     HICEKCKNLMELQSEETEDTWLKEYQIAEEKIKSVDYDVWVCRKCEYIRIEPYFHAGA
                     YDKCPKCSAIGLRFIQDKVITPATYSSSGSGVKIYKCSICKHEDKRNYTIPKKVKSSS
                     SSGGYSSGGGSSYSSSSSSSSSSSSSSSFGGGSSGGGGSSGSW"
     gene            complement(7940..>9637)
                     /gene="uvrB"
                     /locus_tag="PLY93_01920"
     CDS             complement(7940..>9637)
                     /gene="uvrB"
                     /locus_tag="PLY93_01920"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRP68268.1"
                     /translation="DYYQPEAYVPTTDTYIEKESSINDEIDRLRLRATASLIERKDVI
                     IVSSVSCIYGLGSPDTYKDAVLMIDTAVPASRDDIMMRLMRMQYTRNDTELLQGRYRV
                     RGDVLDVFPAYANDPIRVEFFGRDIEAIYRFDLLTGKKKEALKRVAVYPAKHFITSPA
                     TLERAIEQMKVELAEQLEYFKQVNKPLEAERLRQRTTYDMEMLREMGYCSGIENYSRI
                     LTGRSPGERPECLIDYFSDDILVIIDESHVTVPQIGAMFAGDRTRKQTLVDFGFRLPC
                     ALDNRPLKFEEFETLATNVLYVSATPAAYELEKSDTHVEQIIRPTGLLDPEIEIHPSE
                     GQIVHLILEIDKRVKVNERVLITTLTKKMAEELTDYLEKASIKVRYLHSDIDALTRLE
                     ILRDLRKGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSHRSLIQTMGRAARN
                     VNGKVILYADVVTDSMQSAIDETTRRRSLQVAFNTKHGITPQTIKKEVKDFVEREKKI
                     ENRQWQLVEENLKEFSRKKYKSTDAWKKAVEKAMFDAAKELNFERAAELRDLLKAED"
BASE COUNT         2520 a         2409 c         2175 g         2533 t
ORIGIN      
        1 catttgtcag gtatgtccgt gggttagaca aatcagtctt aaagaataaa aaacatctta
       61 acttccacaa attgatggca agttcaagag ttaacgcacc gatgttttag ttaggtatca
      121 tgcgttattt tatttgttgt attctcgcct ttacatttgt aaacaccgtc gatgcaaaaa
      181 agcatttcaa ggtaaggcca ggcctcgaca cgttgatgca aaaaagcgac aagtggctca
      241 agggcaaacg tatcgcgctt atcgtcaacc atagcgccgt cgactctaaa gggcggcaca
      301 tcctcgactt gctttatccc caatataaag tcgtaaaaat tttcgcaccc gagcacggtg
      361 tgcgtggcgc actcgacgaa cacgtcgacg atgaaaagga cactaaaacg gggatcccca
      421 tcgtctcgct ctaccagcgg aaaaagaaag cccccaccaa cgaagacttg gcggatgtgg
      481 atgtccttat tttcgacatt caagaaatcg gactgcgcta ctacacgtac gcaagcacga
      541 tggtgatggc gatgaaagcg gcgaagagcg cgggcaaaac catgtatatt ttagacaggc
      601 ccaacatggc aggtcccttg ggggtgtatg ggccactttt ggaaaaaaaa ttccacggtg
      661 gttttgcggg ctattacccc ataccgattg cgcacggcat gacgatgggc gagcttgctc
      721 tttattacaa caaaactttt gacattggcg cgaacatccg cgtcatgaaa atggcgaact
      781 accgtcgcgg catgtattac gacgaaaccg gtttgccgtg gaagaatccg agccccagca
      841 tcgtgcgcat ggcttcggtc attggctatc atttagcggg cgcgtttgaa acgctgaaca
      901 tcagcgtggg gcgtggcacc gaccatccgt ttgaggttta cggcgcgcct ttttttgacg
      961 cccaaaaaat cacccgcgaa ctcacccaag caaaacttcc cggtctaaaa tttacaccca
     1021 ccacgtttac gcccacccgt tctgcattca aaggtaaagc gtgccatggt ttcaagctcg
     1081 caatcacaaa ccgtcgaaaa atccaaccga tgcgcaccgt gatcaccatc ggccgcatcc
     1141 tctataaaaa tttacccgaa gaaacgcgca aagcaaattg ggcgcgggtg gcgaatggca
     1201 tcggcgacac tgcgttcatc gagcgaattg ccaaaggcga aaattacaaa aaacttttaa
     1261 aattactcaa gaaagaagtg cacggtttta ttaaatcgcg caaaccctat ttactatatc
     1321 gttaaagcgg gcgtcgcccg ctttaacgat atgcttatta agcgctcgcc atcatgaaga
     1381 cttcttgttc gacagcgcgt tctttttcgc ccatcgtcgc ttcacggtct aaatacgctt
     1441 gaaaggtatg gccgtcgcgc gtcgccatac agtacgcgtt aaagagcatg ttgatgaagt
     1501 ctttcatcat cgtttgcggc atcggcaggt ggaagcccac acggtacgcg aggttcaaca
     1561 ctttgcgcac gagcgaaccg ccgaaatcaa acacgtcgat gcccagctcg gcaaaatctt
     1621 tttccatttt aaaagccaat gggattgcag gcaacaccag cgagaaacgt ttcagcatcg
     1681 ctgcggggtt gtttctttcc caccaggtga gcgggcggtc ttttaagtag tttgtcggta
     1741 gcgcacagtg gcgggattcg tctaaatgga acatctccag aatttcaagc cctggaaatt
     1801 tgctcaacgc gccaaaaatg gagagagcga ttccctcaac gagcacgttc attccaaaaa
     1861 atttttcaag gcctttcgac ttgagcgtgc cttcgaggat taagtattca taccccgtga
     1921 ggcgcgggat cggcatctcg aacgcttgga tgagctcgcg caacaccacg aagtgtttcg
     1981 cctcttccat cacctgcatg gtgagcgcgg cctttgcgcc tgtgctcttc acgtcgcgca
     2041 acacttcgtt tgagacgagc catgcgtacg cttcgccgtg gccaatcatc gaaaggatgt
     2101 tcacaagcgc ttgcttttgc gcaggtgtgt actcgcgcgc caacaactcc ttgaactcgg
     2161 cggatttcat ccgttccagt tgcttttttt cgccttggtt taacgattcc ccagccattt
     2221 taagaagcat cgcttcttct ttatttaagt cattgaaggt cgaccattcc gcgtgcttct
     2281 ccgctttcca aagcaaacgc agacttttat cgtagtgttt tttacggagt tcgtcgtttg
     2341 caccgcccat aaattcatag ttcaagtcgt cgtaatcggc ggcacgaccg cctaagccga
     2401 cagtgcccat gagcgtgtcg aagccacgca cggcaaagtt tacatacgcc tcgagcgtgt
     2461 cgttgacacg ttgtgcattt tcattcactt gcagtttttg aaatttcata aatccccctg
     2521 gggcaagccc caaagaaatc taaaaaaggc gcacattttg tcaataaaaa aactataact
     2581 acctatatag tttttagagt ctatacacct ataattatgg aataaaaagt tcactcgtat
     2641 tttactcaac cacggcaatg caataaaagc tctggttggt attacacgct gccgcacctc
     2701 cctcagtcca gtcacccgta gtggcggaag gagtgcctgt ttgtccctga acaccacttg
     2761 aattggaagt ccagcggttg cagtggctac tcgcaagggc tcccgttgca gtggtacccg
     2821 tccataccgc ccccatgggc gcatctcccc tctcatcata aatggctgcc gtgtgcgcta
     2881 tactgaaaag ttcactggtt ccgcttgcga caatttcatt tttagtgtta taccaaactg
     2941 cgtcggacgt catcgaacaa gccgagcttg cgacggaaaa taagcgacag ctcatatcgg
     3001 caaggtcatc gctgaggaat gcaatataag taccgccaaa tccgtactgc tgcgccgttt
     3061 gttggcaaag acaatttgca ttttgtatac ccacgccatt gcatgagaaa ttactcatgt
     3121 taccgttaga cagggtcgta gttacaaaaa ttcgttttac cggaggtggg gtattgctta
     3181 tagcgcctgg attttgaagg ctttcgagta agccataatt attacacgcc gaaaagacaa
     3241 gtgcaaaaaa tacagttaaa gacaatagtt tcataagact actataactc tacaagaacg
     3301 attgtcaagg aaaagagatg cagagaatag tcgaccaccc aaccttttga tccggttcgt
     3361 aatcatccac taatgcgtcc cctaggggaa tcgaacccct gttgcgggaa tgaaaatccc
     3421 gagtcctaac ctctagacga aggggactaa tccttctatg ggcggcatgg atgcctacat
     3481 ccgttccgtc cactgtgaga agcccgggaa tcgaacccgg gacccactcc ttaaaaggga
     3541 gttgctctac cgactgagct agcttctctt aacatgcccg aactacacgg acaaagccct
     3601 caatcttgta gggcacaacg tcatggcaat tcctataaat tcgtcattgc gtcaaatctt
     3661 ttgattgtct gtaggaagaa atttttagag ttttgcataa ttctatcttc atttcatata
     3721 cgtgaatata atttgcgtcg tgtcgcaaaa gtccacagtg ccgaagatgc aaataccgca
     3781 tttacctgcc aagcctcact acttgtcctt cgacactgcg ctggactttt ttaccctttt
     3841 tgggcgtttg gaagcggcgt tttccaattg ttttttgctc gaatcgttgg gcgaacaaag
     3901 ttatgattcc cgctactcag tgattggttt cttacccgat tttgatttca tcgccacaca
     3961 cgacaaactc acaatccgca gtgtcgacac aaccgaagaa gtaatcacaa aaaatccata
     4021 ctactacttg cgtagtattc tgcagcaaga cgttttaaca cgacactatg ccggcggcct
     4081 tgtggggtat ttagggtacg acgcggccaa tttttttgaa ctgtcgctac aattaaaacc
     4141 gcaccccgat ttccccgcgt tccacatggg gctttacacc gacggactca tcttcgacaa
     4201 actcaccggc gaaacgacgt atttttattt cttgaacgac cgccgcaatg aaattgtgcg
     4261 ggcaatccaa acgccgcgcc ctcctaaacc aacgcgcgtg acgccgttgt cgcaatccaa
     4321 atcacgcgaa gaacacgcac gcatggtcga ggcggccaaa gaagaaattc tcgccggcaa
     4381 cacattccaa gtacaaattg gcttgagcga atcctataaa gtggaaggcg actggctacg
     4441 tctctacgaa agcctgcgca ccatcaaccc ctcgcctcac atgtttttta tgaaattttt
     4501 tgacggtgaa aaaaatcatt atcttatcgg tgcaagcccc gagctgttgt accgccaacg
     4561 gcaaggtgaa atggaatcgt tcccgcttgt gggcaccaca aaacgcggca ccaaaacgaa
     4621 gaccgcgaat ttgtgcgcca actcctctcc aaccccaaag aaattgccga gcacaacatg
     4681 ctcgtcgatt tgcaccgcaa cgacttaggt cgcgtggcac gtttcggttc ggttaaagta
     4741 cgacgtattt ttgacatcaa aaaattttcg cacgtacagc atatctccag cgaagtggtt
     4801 ggtctcatcg cccccaacca agatatgttc acagcgcttg catcgtcgtt tcccgcgggc
     4861 acactttccg gcgcgcctaa aattgaatcg atgaaaatca tcgagcgcat cgaacaagag
     4921 ccacgcggcc cttacggagg cgcgattggc cattttggtt tcaacggcga ttccacattt
     4981 gccatcccca tccgcacaat ttttggcgca cgggacaaag cgtttgtgcg cgcatcgggc
     5041 ggcgtggtgt atgactcgac cgccgaaggc gagtaccaag aaatccaaaa caaactcgcc
     5101 gcttcaaaaa aagcgctcgc ggagtttata tgaaaacgct catcatcgac aactacgatt
     5161 cgttcacgta taatttatac caatacgtgg gtgaagtttt agaagaaaaa aacaaagtat
     5221 tcacggtgga tgtttttcgt aacgatgaga aatcatacga agaaataaaa agtgcagcgt
     5281 acgaccgcat tattatttcc cctggcccag gccatcctgc cgatgcaaag tattttggcg
     5341 tgtgcactca cattttagag aacgcaacaa aagacacaca ctttaaaatt ttgggagtgt
     5401 gtttgggtat gcagggcatg gcgcacattt ttggcggcaa ggttgtaaaa gcacaccaac
     5461 caatgcacgg caaaacctct cccatcaagc acgatgccaa aggtgtgttt gccggtttgc
     5521 cgcagggcat cgaagtgatg cgttaccaca gcctcatcgc cgaagaagcg tcgctccccc
     5581 cgtcgctgga aatcaccgcg cgcaccgccg acggctcgca tgaaatcatg gggctgcgcc
     5641 acaagacact ccccatcgag gggatacaat tccaccccga atcgtttgcc accgagggcg
     5701 gcaaggtgat gctcagaaat tttttaagcg cttgaattat tttctctgta tgagctcccg
     5761 attgtcttga gcgtccttca aaatatactt tccgcccatc cacgcagtga gccaatttct
     5821 ctgccctcgc cattcttctt gtgctttttt ataccggcgc aacgccacca cctcgcggtt
     5881 atagccaccc gaaagttcgt ctaaaatccg ctggctgacg ctgtcgttcg caatcgtcag
     5941 tttggcgata attttctcaa gggggatttt ctcaaaaacg atttgatatt gttgatccaa
     6001 cacaattttg tttcgctgta aaatatccgt cgtgcgttgc cgttgcaaca taacctcgtt
     6061 gtacgcattt gttaaatcac taagtgcact gcgggcattg ataaaactaa tcaaggaata
     6121 aacgagaaag gcaaacccta cgccgcctgc aatgagcgtc tttggatatt ttttgaaaat
     6181 attgccttca aaaaaaggag tcttggcatc gaccacatac gtttgtatcg ctgaaggaag
     6241 ctgttgcgag ccgcagtgtt tgcacttata tttccccgac atcaagagtt caaattcagt
     6301 gccgccacaa ctcgcgcagc gggtcatctg cttttcactg ctcattttta ttcctttaat
     6361 ttaccagcta ccgctcgacc caccccctcc tgaagagcca ccgccaaaac tgctacttga
     6421 agaagaactg ctacttgaag aagaactgct gctatacgac gatccaccgc ccgaactata
     6481 accacccgat gatgaactgc tcttcacctt tttgggtatc gtataatttc gtttatcttc
     6541 atgtttacaa atcgagcatt tataaatttt tactcccgac cccgaggagg agtatgtcgc
     6601 aggagttata actttgtctt ggatgaatcg tagcccaatt gccgaacatt tgggacattt
     6661 atcataagcg ccggcgtgga aatagggctc gatgcggatg tactcgcatt tgcggcaaac
     6721 ccaaacgtcg tagtcgacgc ttttgatttt ctcttctgca atctggtatt ccttaagcca
     6781 tgtatcttcc gtttcttcac tctgcaactc cattagattt ttacattttt cacaaatgtg
     6841 tttgccacgg cggattgctt gacgtgtcca aaaatagatc ggaaaaataa gtattgctag
     6901 aataagaaaa ggtatagctg cataatactc cgaaggactg cgccagaaaa aaaatacggg
     6961 aacaacggcg gcgattccaa ccaagataaa tagaaaaaca taaaatcttt tgagagaaac
     7021 aggagcttta gtaatcgagt tccaattttt atgaatgatt accatagcga taatttcact
     7081 cgccaaaaaa aacgacttaa agaaccacga ggtatagaag aaactcaaaa tgattcctat
     7141 tgccgcaaca aagagctgca tgaaaaataa atgtatcgtt tttgcttttt caaggctgtt
     7201 gggttttgac gcggtttgca aaataaaaaa aactaccact aaaaaaattg caatatgcgc
     7261 aaaaataaaa acggtgtcac gaaggttgta cgttgccttg ccgatttcta actggaatgg
     7321 cgctttggca cccaactctt ctttgggggt tgcatcaatt tttttttcaa tttcgttaat
     7381 cgcatcggca atccctttgt agtaatcccc tcgccgaaac tcgggtgcca tcgtgttgcg
     7441 gccgagactg cctgtgaagc tatcgggcag tgctgtctct aacccataac cgacctcgaa
     7501 tgcccattgc cgttggttca caagcactac caacaatagc cctgtattgg tttctcgatt
     7561 gccaattttc caagtgttga aaatttggtg tgcataatct tttgcgcttt cgacaggtag
     7621 gttttgtttg aaaataatcg caaactcgat accagttttt tggttgatct tttctaaaag
     7681 ttgatttaat gcacctacct gttgtgcagt catgacgctt gcatcgtaag taatccacgc
     7741 attgctacta cgcggatttt ttatttgctg gatggcgtct tcaataggga gagcgtgaag
     7801 acttaaagag agaaaacaaa gtacgggcaa aatttttttc atcgtgtaaa atgccttcgt
     7861 gtgtatataa tgtcaagtgt gaatcatttt gccaggctgg cgcacaggtt ccctgtaagg
     7921 acgattttgg ggtgcgctct tagtcttcgg ccttgagtaa gtctctaagc tctgccgccc
     7981 gctcgaagtt gagctctttg gccgcatcga acatcgcctt ctcgacagct tttttccaag
     8041 catcggtgga tttatatttt ttacgagaaa attctttgag attttcttcg acgagctgcc
     8101 attgtcgatt ttcaattttt ttctcgcgct cgacaaaatc tttgacttct tttttaattg
     8161 tctgtggtgt gatgccgtgc tttgtattaa acgctacttg caagctgcgg cggcgcgtcg
     8221 tttcgtcgat agccgattgc atcgaatcgg tcacgacatc tgcatacaaa attactttgc
     8281 cattcacgtt acgcgcggca cgacccatcg tttggatgag agaacggtgc gaacgcaaga
     8341 aaccttcttt atctgcgtcg aggatggcga cgagcgatac ctcaggtaaa tcgagtcctt
     8401 cgcgcaacaa gttaatccca acgagtacat cgaactcgcc tttacgtagg tcgcgcaaaa
     8461 tttcaagacg ggtaagcgcg tctatgtccg aatgcaaata gcgcacttta atcgacgcct
     8521 tttcaaggta gtcggtgagt tcctcggcca tttttttggt gagagtggta atcagcacgc
     8581 gctcgtttac tttcacgcgt ttgtcgattt ctaaaatcaa gtgaacgatt tgaccttcgc
     8641 tgggatggat ttcaatttca ggatcaagta gccctgtggg tcgaatgatt tgttctacgt
     8701 gcgtatcgct tttttcaagt tcataagccg ccggcgtcgc agaaacgtag agcacgttcg
     8761 ttgccaacgt ttcaaattct tcaaacttga gcgggcggtt atcgagcgcg cacggtaaac
     8821 gaaaaccaaa atcgacgagg gtctgtttgc gcgtgcggtc gccggcaaac attgcgccaa
     8881 tttgcggcac ggtgacgtgg ctctcgtcga tgataacgag tatgtcgtcg ctaaaataat
     8941 cgatgaggca ttcgggtctt tcgcccggag atcgccctgt taaaatacgt gaataatttt
     9001 caataccgct gcaatagccc atttcgcgca acatctccat atcgtaagtg gtacgctggc
     9061 gcaaccgctc cgcttccaaa ggtttattca cttgtttgaa atactcgagt tgttcggcga
     9121 gctcgacttt catttgttcg atagccctct caagggtagc gggtgaagtg atgaagtgct
     9181 ttgcaggata caccgcgaca cgtttcaatg cctctttctt cttgccggtg agtaaatcga
     9241 atctgtaaat cgcttcgatg tcacgcccaa aaaactcgac ccgaatggga tcgtttgcat
     9301 acgccggaaa aacatcgagc acgtcgccgc gtacacggta acgcccttgc aaaagttcgg
     9361 tatcgttgcg cgtgtattgc atccgcatga ggcgcatcat aatgtcgtcg cggctcgcgg
     9421 gcacggcggt atcaatcatc aaaacggcat ctttatacgt gtcgggcgaa cccaaaccat
     9481 aaatgcagct caccgatgag acgatgatta cgtctttgcg ctcgatgagc gacgccgtgg
     9541 cgcgcaaacg caagcggtcg atttcatcgt taattgacga ttctttttcg atgtacgtgt
     9601 cggtggtggg gacatatgct tcgggttgat aataatc
//
LOCUS       DAOMPD010000030         9441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3128_length_9441_cov_2.606435, whole genome shotgun sequence.
ACCESSION   DAOMPD010000030 DAOMPD010000000
VERSION     DAOMPD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3128_length_9441_cov_2.606435"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>266
                     /locus_tag="PLY93_01925"
     CDS             15..>266
                     /locus_tag="PLY93_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRP68269.1"
                     /translation="MNLPCEVGGGIRRLEDFQFYFDNGFELGVDFIMVGSLPFLDRAE
                     FERIAQKFSSSLLMTVDAWGHEVKHSGWLKDSGYTVERLI"
     assembly_gap    267..288
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <289..546
                     /locus_tag="PLY93_01930"
     CDS             <289..546
                     /locus_tag="PLY93_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRP68270.1"
                     /translation="NYLVTQIKKDGMLEGPDFELYDFLLSRFPEIHLIASGGVSSMAD
                     LVKLNEKNLYGAIVGRAFYEGKITAPMMRAFSQSIRQQSKI"
     gene            complement(543..1355)
                     /locus_tag="PLY93_01935"
     CDS             complement(543..1355)
                     /locus_tag="PLY93_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68271.1"
                     /translation="MNSKRFDFQDLEEIDFSDELPAATRFADGEKLFLSTYTQDELWQ
                     ALEKAHIIAELKARGYNSLKLTIEFLTDRDHRFYLTYQDEKILHMRLRLSHFKLHRHP
                     LMSATKMLFIDWLQARHILKVHQKNPRRPWEERLFPGQDVPGLGIFNRLEIFLRNLVV
                     DTHAYGGFNLPEYFHDALLFHRAFRFYDPSREAFFRALLRDLKGHGAREISRALSAGK
                     ITDKKKNLVEWHGGEMLIFTHQPYEEIIFNERYYKDVENKMRDYKFTITGSS"
     gene            complement(1352..2236)
                     /locus_tag="PLY93_01940"
     CDS             complement(1352..2236)
                     /locus_tag="PLY93_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRP68272.1"
                     /translation="MLRKPGHLVYSPRYDFSDYNHVLSAEKYRMLYDALKYERWFWNE
                     PTLIDEEALARVHTKKYLKDFLSARLTEKTKRSEIPIDKRIVEGVLTATQGTLLAAEL
                     ALKHGVASNLSGGFHHSFADHAEGFCYLNDIVVAIRALRVKEPNMKVAVIDLDVHQGN
                     GTAVLLQKDENTYTFSMHEKDNYPLKEQSSHDVELASHIGDKEYIKLLDENLSILKEV
                     ITPDIIFYVAGVDVFQEDILGGLALTPRGIAARDRYVKNFMPKVPIVTVTAGGYARNI
                     ADSVELHAQTIRIFHDRL"
     gene            2437..3501
                     /gene="thrB"
                     /locus_tag="PLY93_01945"
     CDS             2437..3501
                     /gene="thrB"
                     /locus_tag="PLY93_01945"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRP68273.1"
                     /translation="MSSYPPLRISVPASTANLGPGFDIWGIALDLRNEFVCTRAARND
                     GSLRLSFQTGDAKTVPVSSLPKKTDADNLFIKTYNYLIKKAGQTPLSYDVNIVVNVPF
                     SRGLGSSSTAILAGLTLANETLRREYQLAYRIEQILEFALEFEPHPDNLTAALYGGWN
                     LCLPKITALIGETAGSSKDKKGTVPSNTAVPQSPLEDGPSFMRLPMKVRAPIQIAGVI
                     PDLKLETRDSRQLIPVTVSRSDLIFQTSRVAALVALLNEEKLSAAHKTAFRTAMEDRM
                     HTSQRAHLIPEMFQIFEDWYTEGALGCFLSGAGSTLLAFWPSSIDVSQLDLAKRFTAK
                     KIQATQRAFQLDSHGLMLLS"
     gene            3547..>4204
                     /locus_tag="PLY93_01950"
     CDS             3547..>4204
                     /locus_tag="PLY93_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRP68274.1"
                     /translation="MRRNTTLMNDLRERFYLLAEHHQVVALKTGTEVEDMDMDEIALM
                     REIAPKSPLTVKIGGPEARRDMRECLAVGVDVLLAPMVESVYSLINFVETAETIMCET
                     NKNVRLAMNLETALAIHNLDAMIASKAFVSLSQVTIGRGDLSKSMNLRVDDEEVLSLT
                     RNVLIKLARQNKLTSVGGGLSVQNISVMAEVLPASRFNTRHVVFNNSEEFAQNAAVNL
                     S"
     assembly_gap    4205..4214
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4399..5472
                     /locus_tag="PLY93_01955"
     CDS             4399..5472
                     /locus_tag="PLY93_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68275.1"
                     /translation="MSVKNLGASCAIVGGGYAGLAVAIELKRRAPHIQVTLFDERAQH
                     QKITQWHKLVRGKKASQYEVNFQTLSRRFHFTFIQKKIHLTALLQNPKSALYQKFDAV
                     VLAQGSHSPMTPANVVTLDTLRNDTAEQEHLRTLDSKKIAVIGGGPTGVQFAFELAAR
                     KNQVLLFEAQERLLPSFDASLGETAVAEAARAKIDLHLNTEFLRFENNTLYARSGSES
                     LRFNADYVLFVPGVRSTPAFNCDSYGRILEIQGKAPHTRFYAVGDNSYFSGRGLNSKS
                     AQAAIRKAQQVAETLRADLQGGNVPEYTYQELGYFVSLGPKSAVGYLFSQKTAFAGRA
                     ALLMKEAVERQYDLYLAGFNVYL"
     gene            complement(5478..6197)
                     /locus_tag="PLY93_01960"
     CDS             complement(5478..6197)
                     /locus_tag="PLY93_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68276.1"
                     /translation="MIRELRTLWGIHLHLKIGARSEKNPSPRGYVTLPLEKKNIIIYF
                     EGYSGNISDATREPISFAPYTDKDKSRALSQKFFIRFVASLNDMVALEKIQTLVLLMS
                     IGYILDKNQFSGIVDPDVFLFVPPTAVRAGLQQFHRKEQLLNTHFVSGFNFYQQNGAA
                     IFFTHGLNRFGAPDLLIDGGDKTLTTNDYATALKEAHTLFLSHIGNIKRQRKLSPLKR
                     SEAIPEQVRAMIGKEVRRVCI"
     assembly_gap    6239..6248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6376..9060)
                     /locus_tag="PLY93_01965"
     CDS             complement(6376..9060)
                     /locus_tag="PLY93_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRP68277.1"
                     /translation="MQIPKKRSALRRKINFLQLKLVEKIYQSPGKILLSVLAVSVVAA
                     FYTFQLFQDVRTDFATLLPENDRSVVHAHEATARIGGTGNIFVSIYSPDFEANKKFIE
                     AFAGELRKYPQDLIKFFEYRTNEAADFFKDYLFYYLTIQELKELRTALQKRFQKTRLG
                     ALGLDLEESNPDAELKKLLNRIFEKYKSQNPFGVNKDGYFTDATGQSLALIIRPAGDA
                     ADVGFSRKIIARINADIKKVNPTKFHSGMEVGLAGTYVALLENFSSIIQETIQTAGIT
                     IFLVLLSIWFFYRSVRMVAMMCAGILVGILVTFMLTYFKIGYLNQQTAFLASIIIGNG
                     INFGLIHLARYVEERAKGVHLRRALFRGVFYTISATFMAAVATSISYGIMSVTTFKGF
                     SQFGFIGGVGMLVCWLTLTLTLPCFIVFFERWRPQKEAKIRKFIETDRARIFSALVQR
                     YRRALTYVMYAIVPVSIIFTAIYFSADRFEYDLKKLSLKTTEAKGTESYYNKRIDKIL
                     GNGNNPSMLLAHSRQDANKIGLELEKRIAAAAASGKPMFISTVRWLDQFYPQQQKEKF
                     QVIRDLRKLFLPKYISFLAKDQRAMGRMALNALKANPFKIEEMPEMLLRNFRETDGTM
                     GRIIYVTASQKAVLSNIKDVIQIGNEISHSVKLAPGKKIGEGEVLFASESMIFSDILR
                     DVSREGPIVTLVCFLAVGAFIWLGFRRLREFLFIYLFLTFGLLSFIAALYLFGVKLNF
                     FNFITIPITIGIGVDYAINLYYRYKVDGEHSIQDALSSTGSAVFLCSWTTIIGYGSML
                     GAKNQAMASFGLMAVIGEISCLVFAMIFMTAYLGRREDKRAKLRESATGSEIPQSAAQ
                     SSSRESTDKKVTPRSAQRKSKSIPKKATKKKINPPKKR"
     gene            complement(9061..9177)
                     /locus_tag="PLY93_01970"
     CDS             complement(9061..9177)
                     /locus_tag="PLY93_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP68278.1"
                     /translation="MEDYPVARLYRDVKLAEIGGGTSEIMKSIIARSESKKS"
     assembly_gap    9178..9187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9188..>9441)
                     /locus_tag="PLY93_01975"
     CDS             complement(<9188..>9441)
                     /locus_tag="PLY93_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP68279.1"
                     /translation="KTFGKAIIKHQAVAFMLSDMIVKYEAARQYLHETAWILDQAGDE
                     LHYIHNGEKIELTARTASLKLMASTYAREVTNLAVQIFGG"
BASE COUNT         2499 a         2326 c         2120 g         2444 t
ORIGIN      
        1 atatacgccg gagcgtgaac ttgccgtgcg aggtcggcgg tggcatccgc cggcttgaag
       61 attttcagtt ttatttcgac aatggctttg agttgggcgt tgacttcatc atggtgggtt
      121 ctctaccttt tttagaccgt gccgagtttg aacgcatcgc tcaaaaattt tcatcgtctc
      181 ttctaatgac ggtcgacgca tgggggcacg aggtgaaaca ttccggctgg ctcaaagact
      241 cggggtatac cgtcgagcgt ctcatcnnnn nnnnnnnnnn nnnnnnnnaa ctaccttgtc
      301 actcaaatca aaaaagacgg catgttagaa ggccccgatt ttgaactcta cgattttctt
      361 ttatcgcgct ttcctgaaat tcatctcatc gcctcgggag gcgtcagctc catggctgac
      421 ttagtaaaac tcaacgaaaa aaatctctac ggtgcgattg ttggccgcgc gttttatgaa
      481 ggtaaaatca ccgcacccat gatgcgtgca ttcagccaaa gcatccgcca acaatcaaaa
      541 atttaagacg accccgttat agtaaactta tagtcgcgca ttttattttc tacatctttg
      601 taataacgtt cgttaaaaat aatttcttca taaggctggt gcgtgaagat gagcatctcc
      661 cctccatgcc attcaaccag attttttttc ttgtcggtga ttttacccgc actcaatgcg
      721 cgtgaaattt ctcgtgcgcc gtggccttta agatcgcgca gtagtgcacg gaaaaacgcc
      781 tcgcgggatg gatcgtaaaa acgaaatgca cgatggaata aaagcgcgtc gtgaaagtat
      841 tctggcaaat taaatccgcc atacgcatgc gtatcgacga ccaagttccg caaaaaaatt
      901 tcaagccggt taaaaatacc caaccccggc acgtcctgac cgggaaagag cctttcttcc
      961 cacgggcggc gcgggttctt ttgatgtact tttaagatat gccgcgcctg caaccaatcg
     1021 ataaaaagca ttttggttgc gctcatcagc ggatggcggt gcaacttaaa atgcgacagg
     1081 cgtaagcgca tgtgtaaaat tttttcatct tggtaggtta aataaaaccg atggtcgcgg
     1141 tcggttaaaa attcgattgt caacttcaag gagttatatc cccgcgcctt cagctctgcg
     1201 atgatgtgcg ctttttcgag cgcttgccaa agttcatctt gggtgtacgt ggagagaaaa
     1261 agcttttccc cgtcagcaaa gcgggttgct gccggtaatt catcgctaaa atcgatttcc
     1321 tccaaatctt gaaaatcgaa gcgttttgag ttcataatcg gtcgtgaaaa atacgaatcg
     1381 tttgcgcgtg tagttctacg ctatcggcga tgttgcgcgc ataaccgccg gcggtaacag
     1441 tgacaatggg tactttaggc ataaaatttt ttacataacg gtcgcgcgcg gcaatccctc
     1501 ttggagtcaa cgccaagcca cccagtatat cttcttgaaa gacatcgact ccggcaacat
     1561 aaaaaataat atcgggagtt atcacttctt taaggatgga taaattttca tcgagcagct
     1621 ttatgtactc tttgtcgccg atatgcgacg cgagctcaac atcgtgactg gactgttctt
     1681 tcaggggata gttatccttc tcgtgcatcg aaaaagtgta ggtgttctcg tctttttgca
     1741 aaagcacggc agtgccgttg ccttggtgga catccaaatc gataaccgcc actttcatgt
     1801 tgggctcttt gacacgcaac gcgcgtattg ccacaacgat atcgttgaga taacaaaatc
     1861 cttcggcgtg gtcggcaaac gaatggtgaa aaccccccga aagattcgaa gccacgccat
     1921 gtttaagcgc gagctcagcg gctaagagcg ttccttgagt cgccgtcaaa accccttcga
     1981 caatacgttt atcgatgggg atttcagaac gtttcgtttt ttcggtgagt cgtgccgaaa
     2041 gaaaatcctt aaggtatttt ttggtgtgta ctcgtgcgag cgcctcttcg tcgatgagcg
     2101 tcggttcgtt ccaaaaccaa cgctcgtatt tgagtgcatc gtaaagcata cggtattttt
     2161 ccgccgaaag gacgtgattg taatcggaaa aatcatagcg gggagaataa acgagatgac
     2221 cgggtttacg cagcatgttt tacacgtatg cgtcaaacgg ggtaatgagg tctatccgtc
     2281 aacaagtggt ttgattatga gcttgtgcgc caactcgctg aggcaatttt tacacactgc
     2341 atgagttggc gcacaagctt ttataagtcc ctctacctat taaacaaagt taaggtaagt
     2401 gcttgtaggc gagttttttc tcttgtttct ggctcggtgt catcgtaccc accgcttcgt
     2461 atatccgttc ccgccagcac cgcgaactta ggccccggct tcgatatttg ggggattgcg
     2521 ctcgacttac gcaatgagtt cgtctgcact cgagctgcgc gtaacgacgg ctcactgcgg
     2581 ctcagctttc aaaccggcga cgcaaaaacc gtacccgtgt cgtcgctccc caaaaagacc
     2641 gacgccgata atcttttcat caaaacatat aactacctca tcaaaaaagc aggccagact
     2701 cctctctctt acgatgtcaa catcgtcgtc aacgtaccct ttagccgtgg cttgggttcg
     2761 tcgagcactg ccattcttgc gggactcact cttgccaacg aaacactgcg ccgcgaatac
     2821 caactggctt accgcatcga acaaatttta gaattcgccc ttgagtttga gccccacccc
     2881 gacaatttaa ccgctgcgct ctatggagga tggaacctat gcctacctaa aattacagcg
     2941 ctcatcggcg agacagcagg cagttcaaaa gacaaaaaag gcacagtgcc atccaatacc
     3001 gccgtgcctc aatccccttt agaagatgga ccgtctttta tgcgattacc catgaaggta
     3061 cgcgcaccga ttcaaattgc cggagtaatc cccgacctta agttagaaac gcgcgattcg
     3121 aggcaactca tccccgtaac ggtttctcgc agtgatttga tcttccaaac gtcgcgcgtg
     3181 gctgcgctcg tcgcactcct caacgaagag aaactttccg ctgcgcacaa gacggcgttt
     3241 cgtaccgcaa tggaagaccg gatgcacacc tcccagcgcg cgcacctcat tcctgaaatg
     3301 tttcaaattt ttgaagactg gtataccgaa ggcgcgctcg gttgtttttt atcgggtgca
     3361 ggttctacac tcctcgcttt ttggccatcg agcatcgatg tatctcaact tgatttagca
     3421 aaacgtttta cggccaaaaa gatacaagcc acacaacgcg ctttccaact cgattcccac
     3481 gggcttatgt tgttatcata aaagtaattt acttacactc tctctaaaaa aagtcgatag
     3541 ataactatgc ggcgtaatac aactttaatg aatgatttac gtgaacggtt ttatctattg
     3601 gccgaacacc atcaagtcgt cgcgctcaaa acgggaacag aggtcgaaga catggacatg
     3661 gacgaaatcg cgctgatgcg tgaaatcgct cccaaatccc cgttgactgt aaaaattggc
     3721 ggtcccgaag cgcgccgcga tatgcgtgaa tgtttagcgg tgggcgtcga tgtgctttta
     3781 gcgcctatgg tcgaatcggt ttattcgctc atcaactttg tcgaaaccgc tgaaacaatc
     3841 atgtgtgaaa ccaacaagaa cgtgcgcctg gcgatgaatt tagaaaccgc attagcaatc
     3901 cataatttag acgccatgat tgcgagcaaa gcatttgtca gtttaagtca agtgacgatc
     3961 ggccgcggcg atttatccaa aagtatgaac ttacgcgtcg acgatgaaga agtgttatcg
     4021 ctcacccgca acgtgctcat taaattagcg cggcaaaaca agctcacctc cgttggtggt
     4081 gggttaagcg tacaaaacat tagcgttatg gcagaggtac tccctgccag ccgcttcaat
     4141 acccgccacg tcgtctttaa taacagcgaa gagtttgcac aaaacgcagc ggtgaatctc
     4201 tccgnnnnnn nnnnctttat tgtgagcagg cactctacga agcgttggca gagatgaatc
     4261 ccacgcgtgc gtctttttac aaagaacgca accgccagct cgaagaaaga cttccccgtt
     4321 tgagagtaaa gcaaacaaca accttgtgag tcaaaagcgc acctcctgtg ccatagattt
     4381 ttcaccaaga ggccatccat gtccgtgaaa aacttaggcg cctcctgtgc catagtcgga
     4441 ggtggatatg cgggacttgc ggtggcgatt gaactcaagc ggcgtgcacc ccatattcaa
     4501 gtcaccctct ttgacgagcg tgcacagcat caaaaaatta ctcaatggca taaattagtc
     4561 cgaggtaaaa aagcatccca atacgaagtg aacttccaaa ccctctcccg ccggtttcat
     4621 tttacattca tccaaaaaaa aattcactta accgctctcc ttcaaaatcc caaaagcgca
     4681 ctctaccaaa aatttgacgc tgtggtcttg gcgcaaggct cgcattcccc catgacgcct
     4741 gccaacgttg tgactctcga tactctacgg aacgacaccg ccgagcaaga acatctgcgc
     4801 acgcttgact caaaaaaaat tgccgtcatt ggcggcggac caaccggtgt acagtttgcg
     4861 tttgaacttg ccgcacgcaa aaaccaagtc ctgctctttg aagcacaaga acgccttttg
     4921 ccctcgttcg acgcgtcgct gggagaaaca gcagtggcag aagcggcgcg tgcaaaaatt
     4981 gatctccatc tcaataccga atttttacgt tttgaaaata acaccctcta cgcgcgctcg
     5041 gggagtgaga gtttgcgctt caacgccgat tatgtgctct ttgtgcccgg cgtgcgttcc
     5101 acccctgcgt ttaactgcga ttcgtatggc cgcattctcg aaattcaagg caaagcaccg
     5161 cacacgcgtt tttacgcggt gggggataat agctacttca gcgggcgtgg cctcaacagc
     5221 aaaagtgcac aagcggccat ccgcaaagcg caacaagtcg ctgaaacact ccgcgccgat
     5281 ttacaagggg gcaatgttcc cgaatacaca taccaagagt tagggtattt cgtttcctta
     5341 ggtcctaaaa gcgctgtggg gtatttattc tcgcaaaaaa cagcgtttgc gggacgcgct
     5401 gcgcttctca tgaaagaagc agtggagcgg cagtatgatt tatatctcgc tggatttaat
     5461 gtgtatttat agaaagatca aatgcaaacc cgccgcactt ctttacctat catcgcgcgc
     5521 acttgctcgg gtatcgcttc gcttctttta agcggggata gttttctttg ccgcttgatg
     5581 ttacctatgt gacttaaaaa tagcgtatgc gcttctttca gcgctgtcgc atagtcgttg
     5641 gtcgtgagtg ttttatcgcc gccgtcgata agtaaatcgg gtgcaccaaa ccgattcaac
     5701 ccatgcgtaa aaaaaatcgc cgcgccgttt tgttgataga aattaaaccc cgatacaaag
     5761 tgcgtgttga gtaactgctc tttgcggtgg aattgctgaa gtcctgcacg caccgcagtc
     5821 ggcggcacaa ataaaaaaac atcaggatcg acgatgccag aaaattgatt tttgtcgagg
     5881 atatatccaa tactcataag cagaactaat gtttgaattt tctccagcgc caccatgtcg
     5941 ttcaacgagg cgacaaaacg aataaaaaat ttttgtgaaa gcgcgcggga tttgtcttta
     6001 tccgtataag gagcgaaact aatcggttcg cgcgttgcat cggaaatgtt gccgctatag
     6061 ccttcaaaat aaattattat atttttcttc tccaaaggta atgtaacgta cccgcgcggc
     6121 gaaggatttt tttcagaacg cgcgccaatt ttgagatgca agtgaattcc ccacagcgta
     6181 cgtaattcgc ggatcatgtc gatgccgttg tatcagggag cggcgtcgta aagcgcccnn
     6241 nnnnnnnnta tttgcgacgg tggcttttac atttttttta gcagcgggta agcggttgtg
     6301 caaaaaaata ttttcgtatt cgcgttcgtt gaaatgagcg tctaaaggaa cgctccgttt
     6361 tttttgtaac ataggttatc ttttctttgg tggatttatt tttttcttcg tagctttttt
     6421 agggatactt ttgctttttc gttgggcgct ccgtggcgtc acttttttat ctgtactttc
     6481 gcgagaagaa ctttgcgccg cgctttgcgg tatttcagaa ccagttgcac tttcccgaag
     6541 ttttgcgcgc ttatcttcac gacgtcccaa atacgccgtc ataaaaatca tcgcaaaaac
     6601 aagacaactg atttcgccga tgaccgccat aaggccaaaa gaggccatcg cctgattttt
     6661 tgcgcccagc atactcccat acccaatgat cgtagtccat gaacaaagaa acaccgcaga
     6721 gcctgtggag gaaagagcgt cttgaatcga atgctcgccg tccactttgt aacggtagta
     6781 gagattgatg gcatagtcca caccaatacc aattgtgatg ggaatagtga taaaattgaa
     6841 gaagttaagt ttaactccaa acagataaag cgcagcaatg aaagaaagta aaccaaaagt
     6901 aagaaaaaga tatatgaaaa gaaattctct caaccttcta aaccccagcc atataaatgc
     6961 acccaccgcg agaaaacaca ccagagtgac aatcggccct tcgcgcgaaa cgtcgcgcaa
     7021 aatgtcggag aatatcatcg attcgctcgc gaagagcacc tcgccctcgc cgattttttt
     7081 cccgggtgca agtttcaccg aatggctaat ttcattaccg atttgaatga catctttgat
     7141 attcgagagg accgctttct ggctcgcggt cacgtaaatg atacgcccca tcgtgccgtc
     7201 ggtttctcta aaattgcgca gcagcatttc gggcatctct tcaattttaa aagggtttgc
     7261 tttcaacgca ttcaacgcca tccgacccat cgcgcgttga tctttcgcca aaaaagatat
     7321 gtacttcggc aaaaataatt ttcgtaagtc acgaataact tgaaactttt ctttctgctg
     7381 ctgtggataa aattgatcga gccaacgtac cgtactaatg aacatcggct ttcccgatgc
     7441 ggcggcggcg gcgatacgct tctccaactc caaaccaatt ttattggcat cttgcctact
     7501 gtgcgccaat agcatcgaag gattattacc gttccctaaa attttatcga tgcgtttgtt
     7561 gtaataactc tcggtgcctt tcgcttcggt ggttttaagg ctcaattttt taagatcgta
     7621 ttcaaaccgg tctgcgctaa aatagatcgc tgtaaagata atcgacactg gaacgatggc
     7681 gtacataacg tacgtgagag cgcgacggta cctctgtaca agggcgctaa aaatgcgcgc
     7741 tctatcagtt tcgataaact tgcgaatttt agcttctttt tgagggcgcc atctttcaaa
     7801 aaagacaatg aagcaaggga gtgtaagtgt gagagtcaac caacacacga gcatccccac
     7861 gccaccaata aatccaaact gtgaaaaacc tttgaaggtc gtcaccgaca tgataccata
     7921 cgaaatcgac gtggccaccg ccgccatgaa cgtcgccgaa atggtgtaga acacaccacg
     7981 gaaaagcgcg cggcgcagat gcactccttt cgcgcgctct tccacataac gcgccaaatg
     8041 gataagtcca aagttaatcc cattaccaat gatgatcgac gctaagaatg cagtttgttg
     8101 gttgagatag ccaatcttaa aataggtgag cataaacgtg acgagaatcc ccaccaagat
     8161 acccgcgcac atcatcgcca ccatgcgcac cgaacgataa aaaaaccata tcgacaaaag
     8221 caccaagaaa atagttatcc ccgccgtctg tattgtctct tggatgatgc tcgagaaatt
     8281 ctccaagagc gcaacgtacg tccctgccaa acccacttcc ataccgctat ggaattttgt
     8341 ggggtttacc tttttaatgt cggcattgat acgcgcaata attttacgtg aaaaacccac
     8401 gtcggcggca tcaccggcgg gacggataat gagcgccagc gattgtccgg ttgcatcggt
     8461 aaaatagccg tctttgttga cgccaaacgg gttttgggat ttatactttt caaaaatacg
     8521 gttgagtaat tttttaagct ctgcatcggg attgctttct tctaagtcca gccccaacgc
     8581 acccaagcgc gttttttgaa accgtttctg caacgccgtg cgtaattctt tgagttcttg
     8641 gatggttaag taataaaaca aatagtcttt gaaaaaatca gcagcttcgt tcgtgcggta
     8701 ctcaaaaaat ttaattaagt cttggggata ttttctcaac tcccccgcga acgcttcgat
     8761 aaatttttta ttcgcctcaa aatcgggaga atagattgaa acaaaaatat tcccagtgcc
     8821 gccaatgcgc gctgtggctt cgtgcgcgtg taccacgctg cggtcgtttt cggggagtag
     8881 cgtagcaaag tcggtacgca catcttgaaa aagttggaag gtataaaacg cagcaacaac
     8941 agaaacagcg agcaccgaca aaagaatttt accagggctt tgataaattt tttcaaccag
     9001 cttcaactgg agaaagttaa ttttccgccg cagcgccgac cgcttcttag ggatttgcat
     9061 ttacgacttc ttcgactccg agcgagcgat gatcgacttc ataatttctg aagtaccgcc
     9121 gccaatttca gcgagcttca catcgcggta aagccgcgcc accggataat cttcgatnnn
     9181 nnnnnnncgc cgccaaaaat ttgcaccgca agattggtga cttctcgcgc gtacgtcgat
     9241 gccatcaact tcaacgatgc ggtgcgcgca gtgagttcaa ttttttcgcc attgtgtata
     9301 taatgcaatt catcccccgc ttggtctaaa atccacgcgg tttcgtggag atattgccgc
     9361 gcggcttcgt acttgacgat catgtcggag agcataaacg ccactgcttg atgcttgatg
     9421 attgctttgc caaacgtctt g
//
LOCUS       DAOMPD010000031         9244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3210_length_9244_cov_2.726847, whole genome shotgun sequence.
ACCESSION   DAOMPD010000031 DAOMPD010000000
VERSION     DAOMPD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_9244_cov_2.726847"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLY93_01980"
     CDS             complement(<1..333)
                     /locus_tag="PLY93_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRP68280.1"
                     /translation="MNPEIIAEYQKKYPEIFAVVEGILERFPELDRFIKSQGLVKEQI
                     DAQAQEVLETIAKDMKPYRETFEVHRTLPKDGKAHGDVLHELKTMQETERKKWHDGYV
                     SGAVYHGDD"
     gene            complement(349..>1582)
                     /locus_tag="PLY93_01985"
     CDS             complement(349..>1582)
                     /locus_tag="PLY93_01985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRP68281.1"
                     /translation="DGLVKFQGYDVVKLAQWVMLTAGMPGLSGKDSIAHILYIKNEMP
                     HLYAQTYKFLEPKDYLNLKLTGLFATAQDAIALHWVTDNRDLNKVAYHPSLLKTLGVD
                     KTKLPDLYFSTDVLGTLLPDAAVDLGLPAGIKVVLGTPDVQSAALGSGAVDDFAAHLY
                     IGTSSWLTCHVPFKKTSAENNMATIPSAIPGKYIIGNEQETAGYVLTYLRDKVFFPQD
                     ALTSQGAPADVYALFNELATSVPAGSDKLIFTPWLIGERTPIEDHTIRGGFFNMSLNT
                     TRAHLVRAAFEGVAYNSRWLLEAVEDFIGRGVPFINMIGGGARSDLWCQIHADVLNRE
                     IRQVADPIQANSRGVALLAGVSLGRFRFSDISSRVAIKNTYKPNAANRKIYDELYGEF
                     KNVYEAHKKIHARLNATH"
     assembly_gap    1583..1618
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1619..1917)
                     /locus_tag="PLY93_01990"
     CDS             complement(<1619..1917)
                     /locus_tag="PLY93_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRP68282.1"
                     /translation="MIDQQYILTIDLGTSGPKVALVSEHGEVLGCEVEATDLILLEGG
                     GAEQNPDNWWQKIAAASKKLLAKELIPLDKISAVAVTAQWSGTVAVNKEGKPLHNA"
     gene            complement(2028..2587)
                     /gene="rnpB"
                     /locus_tag="PLY93_01995"
     ncRNA           complement(2028..2587)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLY93_01995"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2801..3790
                     /locus_tag="PLY93_02000"
     CDS             2801..3790
                     /locus_tag="PLY93_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRP68283.1"
                     /translation="MQSYEDLEIQGLLKLSRFKRRHFVPLHTSVKTNLYIPPFSNYFL
                     RSNAIFHGVDFKIPKTTRLPRVVTIHDLGFFEESFSTMSFLTQKRAYCNYLITRLKPN
                     RIIVPSEFIKEQLITRFPITREIVRVAYHGSEQAMEAVINNQWNKELEGLGQFILCPG
                     TVEIRKNCLRLIEAFEKTKASHTVSLIFAGGAGFGYEEIRERAAASPLSKRIHFYSEL
                     SRAKFSALYKNALFTIYPSVYEGFGLPIVEAFALGSPLITANIGAMLEVAGDAAFCVN
                     PYSVEELTGAIDILANDDSVRNDLAKRGHERAKFFTWKKAAIITRGVYEELRA"
     gene            complement(3954..4802)
                     /locus_tag="PLY93_02005"
     CDS             complement(3954..4802)
                     /locus_tag="PLY93_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRP68284.1"
                     /translation="MIINNNLSAINAHKVLKFNEWQTDKTIAKLASGERITKAADDAS
                     GLAVSEKMRTQVQGLRQAERNTEDGISFVQTADGYLNQLGSLLQRIRVLSIQAANGIY
                     TADDRQLMQVEISQLVDEVDRVASQAEFNRFKILQGDFARSSKTASMWFHMGANMHQR
                     ERVFVGTMTAAALQLKENNAIRSLSTTAKANNLIGFVDEALSRLMKQRADLGAYSNRL
                     EMSAKGLMNAYENIQASESRIRDADMAEEMVRFTTGQILQQSGTAMLAQANQKPVSVM
                     RLLGRE"
     gene            complement(5090..5927)
                     /locus_tag="PLY93_02010"
                     /pseudo
     CDS             complement(5090..5927)
                     /locus_tag="PLY93_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
     gene            complement(6157..>6612)
                     /locus_tag="PLY93_02015"
     CDS             complement(6157..>6612)
                     /locus_tag="PLY93_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68285.1"
                     /translation="DALQNFEVSELNLEEVMEFIGNATQVSTSSVLDDLAQLGKDEMH
                     RLRRLRTGFRSRVVKKASSNAALYLLIAKGQLASPALFDASARFIFPPYEINSSLEVL
                     RQVREKPVYDVDYDIDHIHLFEEKCLETWLQEAKAERAQVKIVCALHFV"
     assembly_gap    6613..6622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6623..8886)
                     /locus_tag="PLY93_02020"
     CDS             complement(<6623..8886)
                     /locus_tag="PLY93_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRP68286.1"
                     /translation="MIPQNTPLDVRRFFRHGAAKEAAIHTLHDCRVARIATAFFEPSG
                     WGILAETLKHKEVRLLIGREEGTSERIENLLDDFFVDVQNGLLRKNPELLRGVLQALK
                     NGQLAIKLNAQKVVTTLDARYIYHHAKLYIADEKRALVTSANFTRHGLELSREAGYVV
                     TDADDVSYFVERFDEYFGKAKSVTEEFIEALEELLELRNPNDVYCRSLLEIYDLPEFK
                     APRDLPMPALYQKPIISRLVHSIGEYGGAFLIASTGLGKTVIAAHTVAALRAQDKITN
                     VVVFSPAGLREMWRQWMHTARVVSEEFSYTALSDEKWAVSKYTSRLMEQLSRARSDTL
                     IILDESHHTRNESDGGDIRRRNQRVLDAVHKGTKILLLTATPYSRGIDDINSQLRLLP
                     KYDQAGEFFVAHKHWKVEGVQEISELSPCTVLTAPTVIRHFTRSDENGLRYIEFQDNA
                     KMYFPHKLNMHSLQFKNPYNNILKKLKQSELLVWRKEVHPEVQGLFGEEVNSGKRNYL
                     FEARLMHQFCSSAEQVKTALDQLSKEGGYTKIRFAKQKELTVLATEILANFKILKDEK
                     LHALIEVVEKHRNEKIVIFCIYRSTARYLEEKLSKKFKTLSIKATADVDADDLDILLD
                     TFAPIANGKIKPGGEENEFTLKIKEAKIDILVATEAISEGFNLQDARVLVNYDLPWSV
                     LHLAQRMGRLMRPWHEPRELFIYNFLPDTMFDEELRHGKAWHTRLERRNQEHQSFASL
                     PVILSKENEAIELES"
BASE COUNT         2292 a         2261 c         2125 g         2520 t
ORIGIN      
        1 atcgtcaccg tgatataccg cacctgaaac ataaccgtca tgccatttct tgcgctcggt
       61 ttcttgcatg gttttaagtt cgtggagcac atcgccatgc gctttaccgt ctttgggtaa
      121 agtgcggtgg acttcaaatg tctcacggta gggtttcata tcctttgcga tggtttccag
      181 cacttcttgc gcctgtgcgt cgatttgttc tttcaccaat ccctgtgatt taataaaccg
      241 gtcgagttca ggaaagcgct cgagaatgcc ttcgacaact gcgaagatct cgggatattt
      301 tttttgatac tctgcaatga tttctggatt catggtaatc tcctttattt aatgtgttgc
      361 gtttaagcgc gcgtggattt tcttatgcgc ttcgtaaacg ttcttgaact caccgtaaag
      421 ctcatcgtaa attttgcggt tagctgcgtt tggcttgtac gtgtttttta tcgccacgcg
      481 gcttgaaata tcgctgaaac gaaaacgccc caacgacaca cctgctaaaa gcgccacccc
      541 gcgggagttg gcttggatgg ggtcggcgac ttggcggatt tcacggttga gcacgtcggc
      601 gtggatttgg caccacaaat ccgaacgcgc accgccacca atcatattga tgaacggcac
      661 accgcggcca atgaaatctt caaccgcttc caaaagccag cgggagttat acgccacacc
      721 ttcaaacgca gcacgcacaa gatgtgcgcg cgtggtgttc agcgacatat taaaaaaacc
      781 gccgcgtata gtgtggtctt caatcggtgt gcgctcgccg atgagccatg gcgtgaaaat
      841 caatttatcg ctacctgctg gtacgctcgt ggcaagctcg ttaaagagtg catacacatc
      901 ggcaggtgcg ccttgagagg tgagcgcatc ttgaggaaaa aagactttgt cgcgtaaata
      961 cgtcagcaca tacccagcgg tttcttgttc attgccgatg atatatttcc cgggtatagc
     1021 cgagggtatg gtcgccatat tattttccgc cgaagtcttc ttaaacggta catggcaagt
     1081 gagccacgac gacgtaccga tgtaaagatg cgcagcgaaa tcatccactg cgccagaacc
     1141 caacgccgcc gactggacat ccggcgtacc gagcactact ttaatgccag cgggtaaccc
     1201 caggtcgact gcggcgtcgg gcaaaagagt tcccaacaca tcggtggaaa aatataaatc
     1261 agggagttta gttttatcca caccaagtgt ttttaagagc gaggggtggt atgccacttt
     1321 atttaaatcg cggttgtcgg tcacccagtg cagcgcaatc gcgtcttgcg cggtggcaaa
     1381 aagccctgtc aacttcaagt tcaaataatc tttgggttct aaaaatttat acgtctgcgc
     1441 atagaggtga ggcatttcat ttttaatata aagaatatgc gcgatggaat ctttccctga
     1501 aaggccaggc atgccagcgg tgagcatcac ccactgcgcc agctttacca catcgtagcc
     1561 ttggaatttg acaagaccat ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     1621 gttgtggagc ggctttcctt ctttgttgac agcgaccgtt ccggaccatt gcgcagtcac
     1681 cgcgacggcg ctaattttat ccagtgggat caactctttt gcgagtagtt tttttgaagc
     1741 ggctgcaatt ttctgccacc agttgtcggg attttgttct gccccaccgc cttcgagtaa
     1801 aatcaaatca gtggcttcaa cttcgcaccc caagacttcc ccgtgctcgc tcacgagagc
     1861 aaccttgggg ccggaggtgc ctaagtcgat ggtgagaatg tactgttgat cgatcatgtt
     1921 tctgtacatg agagcggcgc ggctctgtaa accgaaatat cgccgaactt ttaaaatgtc
     1981 tgatgccgat ataagcggcg ccatggaact cccaagagtg cctaaaaaaa atataagcaa
     2041 tcataagccg gattctgtat cgagtctgcc gaatgtaaga taaaatttat cgtttttttg
     2101 tgagctacgc gcacaaaaaa actaaattta actcttattc ggcatacctt tgcaaccatt
     2161 tatcttgccg ccgcgttata ccgtggcgtt ctttgctttc taccatttgc gtatctcggc
     2221 gggaacgcgc caagcaatgc tctaaagaga cacacaaaat cttgaaattg cacccggggg
     2281 agtttaccct gcctcgcgcc tctcggacga gcggtgcgct cttaccgcac cttttcacct
     2341 tttccctggt gccctgacgg gcaccggtag acaaaagatt ttaaggggat agctgcgcta
     2401 cccccttaac aacccccgcc tagggtagtt tgttttctgt ggcactgttc gttgccgtat
     2461 gcgtttatga tcgcgtagcg gtcataaacg tgatacaaca cccagatttc tctggccccg
     2521 tgttccgtgg tgtccggact ttcctcgctc cttcctaaag aaggccgcgc ggttgctcat
     2581 gcttgtagga aatatacaac aaaagagaca aagcgtcacg tagagtttga agtttttact
     2641 aaaaagagat ttacgtcatg cgcaatgaat tttcttcaat taaattgacg ttcagcgtgc
     2701 ggaacaaata cggattatgt ttatctattg ggatgcaaat gtttttgccg cacagcacat
     2761 caccggcatt ggcgtctatg ccaaatattt gctacgcgcg ttgcaaagtt atgaagacct
     2821 tgaaattcaa ggcctgctca aactgagtcg atttaagcgc cgtcattttg ttccactcca
     2881 cacaagcgtt aaaacaaatc tctatatccc tcctttcagt aattattttt tgcgttccaa
     2941 tgcaattttc catggcgtgg attttaaaat tccaaaaacg acacggcttc ctcgcgtagt
     3001 cacaattcac gatttaggtt tttttgaaga aagctttagt acgatgtcat tcttaacgca
     3061 gaagcgtgct tattgtaatt atttaatcac tcgattaaaa cctaaccgta tcattgttcc
     3121 atcagagttt attaaagaac agttaataac gcgctttcca attacaagag aaatagtccg
     3181 tgttgcttac cacggttcag aacaagcgat ggaagcggta atcaataatc aatggaacaa
     3241 agaactggaa ggtttgggac aatttatctt atgtccaggc actgtagaaa tccgaaagaa
     3301 ttgccttcgg cttattgaag catttgagaa gacaaaggcg agccatactg tttctttaat
     3361 ttttgccggc ggtgctggtt ttggatatga agaaattcgt gagcgcgccg ctgcctcacc
     3421 actgtcaaaa cgtattcatt tttattctga actttctcgc gctaaatttt cagcgttgta
     3481 taagaatgcg ctttttacta tttatccatc ggtttatgaa ggtttcggcc tacctatagt
     3541 cgaagcgttt gcgttaggct cccccctcat aacagcaaat atcggcgcaa tgcttgaagt
     3601 ggctggtgat gctgctttct gtgttaatcc ttattcggtg gaagagctta caggggcgat
     3661 agatattctt gctaacgacg attcagtacg caacgattta gcaaaacgag ggcacgaacg
     3721 tgcaaaattt tttacatgga aaaaggcagc cataataaca cgtggcgttt acgaagaact
     3781 ccgtgcgtag ctaagcgcct gcgcgtcaac tcgcgcagca tgtacaaaaa aattctctca
     3841 gcgagttggc gcacagactc ctaatcggtt gcacaaacaa aaaaagcccg ggcttacccc
     3901 ctcaacaacc accgaggcgg ttgtcaaaag aataagtacc cggactccgt caactattca
     3961 cgccccaaga gacgcatcac cgaaacaggt ttttggttag cctgggctaa catcgccgta
     4021 ccactctgtt gaagaatttg gccggttgta aaccgtacca tttcctcggc catgtccgca
     4081 tcgcgaatac gactttcaga cgcctgtata ttttcataag cgttcatcaa tcccttcgca
     4141 gacatttcaa gccgattcga gtaggcgccc agatctgccc gctgcttcat gagccgcgac
     4201 agcgcttcgt ccacaaagcc aattaggttg ttggcttttg cggtggtcga taacgaacga
     4261 atcgcattgt tctctttcag ttgaagagcc gctgcggtca ttgtaccgac gaacacacgt
     4321 tcgcgctggt gcatatttgc ccccatgtga aaccacatcg aagcagtttt cgaggacctt
     4381 gcaaagtcgc cttgcaatat cttgaaacgg ttgaattccg cttgcgacgc gactcgatcg
     4441 acttcgtcga caagttgtga aatttcaacc tgcatcagtt gcctgtcgtc tgcggtgtaa
     4501 ataccgttgg ccgcttggat cgacaagacg cgtatacgtt gcaacaaact gcccagttgg
     4561 tttaagtaac cgtcggcggt ttgtacaaag ctaataccgt cttcggtatt tctttctgct
     4621 tggcgtaacc cctgcacttg ggttctcatt ttttcagaga cagccagacc cgacgcatca
     4681 tcggcagcct tggtgatacg ttcacccgag gcaagcttcg cgatggtctt gtctgtttgc
     4741 cattcattga acttgagaac cttgtgtgcg ttgatggcgc taaggttgtt gttaatgatc
     4801 atagacactc ctttgtcttt attttgtggg ctatacaccc atcaatgtag tgtctgtgct
     4861 cgagacataa gagaggcatc cttgccgtcc tcttttgtct accttagaca agtctctccc
     4921 agtcagacac cttcgcggat taccaaagcg gtaatgcaat tacagttttg ataatccgtc
     4981 agacgcctga cgtgaaagag ggcacaataa aatttcagac acgaaaaggc agcctatgct
     5041 gcccatcgta gtaataaaaa ataatgagcg gctacgccgc tcattatttt taacgcagga
     5101 gttgcagcac cgactgcggt tttgcattcg cttgtgcgag catcgccgta ccgctctgga
     5161 tgagaatttg gttcttggtg aactttgtca tctcttctgc catgtcggta tcgcggatgc
     5221 ggctttccga cgcttgaata ttttcgtaag cgttcatgag tcccttcgcg gcatgctcta
     5281 agcggttgta atacgcgccc aagtttgccc gttggcgact caccatttgc aacgcatcgt
     5341 ccataaggcc aatcatgctg tttgccttcg acgcggtgga aatcgattcg ataccgcccg
     5401 cttgcgaacg taaattgagc gctgcagccg tcatggttgc aatgaaaaca cgctcgcgtt
     5461 ggtgcatgtt tgcccccatg tggaaccaca tcgatgcggt tgccgacgta cgccccttga
     5521 aaaagtttca tcgtgttgaa ctcggcttgc gaagcgatgc gatcgacttc atcgacaagc
     5581 tgcgagacct ccacttgcat catttgacgg tcttcggctg tgtagatacc gttggaggat
     5641 tgcacaccca acacgcgaat gcgttgcaac agctctgaaa gctcgttcaa atagccttcg
     5701 ctggtttgaa tcatgctcat gccgtcttcg gtgttccgct ctgcttgacg aaggcccgcg
     5761 acctgcgtac gcattttttc agacactgca agaccggatg cgtcgtcgcc accacggtta
     5821 atccgcatcc cgctcgacag cttttccata ctcttcgaca gttcttcgtt ttgaaatttg
     5881 agcacacgat gtgagttgat cgcgctcagg ttgtggttga taatcattta accctccgtt
     5941 gtccttaggt ggcccaccat ccttggtagg tcatgctcta actacggagt ttaggttatc
     6001 caagtggaac caactcacct caaaccaggc taaattgaac aaggttaaat taaattaatg
     6061 ccataaaaca cctatgtttc acccacttta gaaaaaaatt atcttttttt ccctaatggt
     6121 aatgtcggcg gattgcaaaa tagtttgagc atttttttac acaaaatgca acgcacagac
     6181 aatcttcact tgcgcgcgct cggccttcgc ttcttgaagc cacgtctcaa ggcatttttc
     6241 ttcaaacaag tggatgtgat cgatgtcgta atctacatcg taaacgggct tttcgcgcac
     6301 ctgccgcagc acttctaaag aagagtttat ttcataagga ggaaaaataa aacgcgccga
     6361 tgcatcaaag agcgcgggcg aagccagttg gcctttggcg atcagcaaat aaagagcggc
     6421 attggacgac gcctttttga cgacacgact tctaaagccg gttcttaatc gacgtaaacg
     6481 atgcatttca tctttaccaa gttgcgccaa gtcgtcgagt acactgctgg tggaaacttg
     6541 agtagcgttg ccgataaact ccatcacttc ttctaaattg agttcgctca cttcaaagtt
     6601 ttgtagtgca tcnnnnnnnn nngactccaa ctcaattgcc tcgttttctt tcgagagaat
     6661 caccggcagg ctcgcgaacg attgatgttc ttgattgcgt ctttctaagc gggtgtgcca
     6721 tgccttgccg tgacgcagct cttcgtcgaa catggtgtcg ggcaaaaaat tataaatgaa
     6781 aagctcgcgc ggttcatgcc aagggcgcat caaccgtccc atccgctgcg ccaagtgcaa
     6841 caccgaccac ggcaaatcgt agttaacgag gacacgcgcg tcttgtaaat tgaacccttc
     6901 cgaaatcgcc tctgtggcaa caaggatgtc gatttttgct tctttaattt tcagtgtaaa
     6961 ttcattctct tctccgcctg gttttatttt tccgttggcg atgggggcaa acgtatcgag
     7021 aagaatatcc aaatcgtcgg cgtcaacatc ggcagtcgct ttaatcgaga gagttttaaa
     7081 tttcttggaa agcttttctt ctaaataacg cgcggtgctg cggtagatac aaaaaattac
     7141 aattttttcg tttctgtgtt tctccacaac ctcaatgagc gcgtgtagtt tttcgtcttt
     7201 gagtattttg aaattagcaa gtatttctgt ggcgagtacg gtcaattcct tttgcttggc
     7261 gaaacgaatt tttgtataac cgccttcttt ggaaagttga tcgagcgccg tcttgacttg
     7321 ctctgccgac gaacaaaatt ggtgcatgag ccgcgcttca aaaagataat ttcttttccc
     7381 agaatttact tcctcgccaa aaagtccctg cacttcggga tgtacttctt tgcgccatac
     7441 gagcaattcg ctttgtttta attttttcaa aatgttgttg tacggatttt tgaattggag
     7501 cgaatgcata ttgagcttgt gcggaaaata catcttcgca ttatcttgaa actcgatata
     7561 gcgcaagcca ttctcgtcgc tgcgcgtgaa gtgtcgaatc accgtgggtg cagtgagcac
     7621 ggtgcatggc gaaagttcgc tgatttcttg tacgccctcc actttccaat gtttgtgcgc
     7681 aacaaaaaac tcgccggctt ggtcgtactt gggtagtaaa cgcagctggc tgttgatgtc
     7741 gtcgatacca cggctgtacg gcgttgcggt gaggagtaaa atttttgtac ccttatgcac
     7801 agcatcgagc acacgttgat tcctccggcg gatatcccca ccgtcgctct cgttacgggt
     7861 gtggtggctt tcgtcgagga tgatgagagt atcgctacgc gcacgggaaa gttgctccat
     7921 cagccgcgat gtgtattttg agactgccca tttttcatcc gagagcgccg tgtacgaaaa
     7981 ttcttcactc acaacgcgcg ccgtgtgcat ccactggcgc cacatctcgc gcaaacccgc
     8041 aggtgaaaaa accaccacgt tcgtgatttt atcttgcgcg cgcaacgccg ccaccgtgtg
     8101 cgcggcaatc acggttttgc caagaccggt ggaagcaatt agaaacgcac cgccatattc
     8161 gccgatgctg tgtaccaagc gcgagataat cggtttttga tacagcgcag gcatcggtaa
     8221 atcacgtggc gctttgaact caggcaagtc gtaaatttcg agcaacgaac ggcaatatac
     8281 atcgttggga ttgcgtaatt ctaaaagctc ttcgagcgct tcgatgaact cttctgtaac
     8341 tgatttggcc ttaccgaagt attcatcgaa acgctcgaca aaataactaa cgtcatccgc
     8401 gtcggtgacg acgtaacccg cctcgcggct caactccaac ccatgccgtg taaaatttgc
     8461 cgaggtgacg agcgctcttt tttcatcggc gatataaagt ttagcatggt ggtagatata
     8521 acgtgcatct aaggttgtca caactttttg cgcgttgagt ttaatcgcaa gctggccatt
     8581 tttaagcgcc tgcaaaacac cgcgcaagag ctcgggattc ttgcgcaaca atccattttg
     8641 cacatcgaca aaaaaatcat ccagcaaatt ttcgatgcgc tcgctggttc cttcttcgcg
     8701 gccaatgagc aaacgcactt ctttatgctt taacgtttcc gctaaaatac cccaaccgct
     8761 gggctcaaaa aatgcggtgg caatccgcgc cacacggcaa tcgtgaagcg tatggattgc
     8821 cgcttctttc gctgcaccgt gccgaaagaa acggcgcaca tcgagaggtg tgttttgtgg
     8881 tatcattgcc agtgtaaaag actatggatg cgttgaggta aactactcaa ggccgatagt
     8941 tggacataaa gatgaaagca ttctgcaaca tttttgtttt gtcaatactt ttgtgatttt
     9001 tgtttaagag cctgtgcgcc agctcggaca gcatatacaa gattttgcaa aaaatcgtct
     9061 cagcgagttc gcggacaggc gctcacaaca ctacttttcc ggttggcgta tagactctaa
     9121 gggatgaagt taccgttgat gcagaaaaaa aagccgacca ccgcaaagaa aaaggctacc
     9181 gcttcggtgt ctaaaaaaaa gaaaaagaac acacccaagc gaacaaataa aacgaagaaa
     9241 gaaa
//
LOCUS       DAOMPD010000032         9232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3214_length_9232_cov_3.034434, whole genome shotgun sequence.
ACCESSION   DAOMPD010000032 DAOMPD010000000
VERSION     DAOMPD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3214_length_9232_cov_3.034434"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="serA"
                     /locus_tag="PLY93_02025"
     CDS             complement(<1..1149)
                     /gene="serA"
                     /locus_tag="PLY93_02025"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRP68287.1"
                     /translation="MVKPKILIADNLDKDGVDILNASGLFEVDYRKKTTREELEAVVG
                     TVDGLIIRSASKVDKALVEKATNLKVVIRAGVGVDNIDIPACSQKGIVVMNAPAGNSI
                     STAEQAIALMFSLARKIPQAHGSMKEKKWEKSKFQGTQLTGKKLGVIGLGRIGKEVVK
                     RAKGLQMEVLGFDPYIPKEHLAHLEIQLMSVDKILKEADFVTVHTPLTETTKGLVNEK
                     NLSQLKPGIRLINCARGGIYDEDALVKGLKEGMIGGVALDVFTVEPPPGTSEFYTLEN
                     VVMTPHLGASTDEAQIEVAKETAESMVTFFKSGLARNSLNFPTIDPSAMDILAPWFTL
                     SAQVGAFIGQMMKRAIREVEITYRGTFTALNLGPLENALTKGLLSVAMG"
     gene            complement(1288..2070)
                     /locus_tag="PLY93_02030"
     CDS             complement(1288..2070)
                     /locus_tag="PLY93_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRP68288.1"
                     /translation="MSLQNDKVARALFGNIYTSFTQGKGIGSLATLLSMLRGLPKSAD
                     GKNYLAPVDIVDLILENEKSLNKMVGVFGELQELVSQNFALFDVTLGELHDIIRSIVV
                     DIKPFTDIRGHRIVVSSLPSRFAGRRLKLNWEFMRKVLYELLINALKFSSPQSTITVL
                     VEYLHTRILITVLNEPYTVNRGEIKGIPEEYRRIIFEPFFRVSRILNEAYDTLEYGLG
                     LTLVDKIIQMHKGSIRNLTIQDFGDGTDEKTDLVAFEIELPA"
     gene            complement(2051..2515)
                     /locus_tag="PLY93_02035"
     CDS             complement(2051..2515)
                     /locus_tag="PLY93_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68289.1"
                     /translation="MSNREPILIVEDDLVQQELIETILNEMPYALTFVDNTKDALDAF
                     DKMQYPVVLCDIYLKGGDSGVEVLKQLFEREIPPVVVMQTIEGGTRQVIECLRLGAYD
                     YIVKPIVPNELRKTIERAFDEVHKCMRLRTLEKERARQLETQQATQRVAAKR"
     gene            complement(2621..>3012)
                     /locus_tag="PLY93_02040"
     CDS             complement(2621..>3012)
                     /locus_tag="PLY93_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68290.1"
                     /translation="EGLRLIAGHVRNLYDLANTDQMVAVRGAIDTAVAPTGALQFMTT
                     EANNSGELIYQVVKDGKDTVMLTVKLQNYRPRPQFINLRRDGRLLQSLPLHEDFAWFA
                     QLTLGEYELELLNNSLNNGKKIDIHIV"
     assembly_gap    3013..3022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3023..3538)
                     /locus_tag="PLY93_02045"
     CDS             complement(<3023..3538)
                     /locus_tag="PLY93_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68291.1"
                     /translation="MQKDTKTQGKSGSKEYAHLLKLYLDEKSRAKAIALAQDDLKSLD
                     MIVQFETMLALNKRNAAAQRATKFALATHEVMEIAEQFFSVDDPHLMEKMLSKNKVWQ
                     KLKTSDADFSLFQDILSFLFTWFEKPLIPTPAYLKQIIMNRLQTAAPAPTTGAIVIRC
                     DRFVLAPMDLAA"
     gene            3638..4315
                     /locus_tag="PLY93_02050"
     CDS             3638..4315
                     /locus_tag="PLY93_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRP68292.1"
                     /translation="MFKSVTENDLKILSQLKKNNDREWFHKHKPKIEIAQKNFLDLVS
                     GLIFVLSEFDESVSNVEPKSAIFRIYRDVRYAHDKSPYKAHLAAFISVGGRLSDTEPG
                     YYFHFAPHGASLFGAGLYMSQKNTLRNVRTEIAHPQSRIVQMLADKVFRKSFPVLDED
                     LKLKRVPRGFDANHPQCELLKLRHHFVYTQLADKVVTSKNLLNTLAKGAEPLSRWCAV
                     LRQATRG"
     gene            4503..6092
                     /locus_tag="PLY93_02055"
     CDS             4503..6092
                     /locus_tag="PLY93_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP68293.1"
                     /translation="MKNYLVLFFVAFAYQPSFLAAEPTPKTSDEKVETKAEKSDKSSE
                     NKPTEATFTIAGTQLSIQDAINIVLEKNLTLQAAKYDVLMTDTNAMKLEKKYAPTLSG
                     EVQHLDFSNSPLDAFNGNYQNNAYVSISKLFATGTTVGGGYRYQQLHSEASNGFLGLP
                     IKTPESTNNFHGYFINVQQELLRNSFGYNDRKMDQIARKQSVAQRAYTINLLSGLVVQ
                     ALTDYWQVTIQKFALENAKLEEKSNRQVRAIVARNVNYGLGESYDLNNYNARVANSVA
                     KVAMTEQSLKNATRKLLRTVNMPVDTQIEGITNLVDVLPNLDADAALKAAMEKRVDLK
                     NAKTELEVAELQSDLYGNQALPSLTAYFNLVSNGTNNMIMFPGFPSAGALQSPQWQVG
                     VRASYPLWDQEIKANKRNANMQLSQARIKIANAEQEIRDDVLTRLENVRLTHEIFQNS
                     RTSRKESEAFYNRMLARTRTGKLNFQLVGQALENMVANRQRELESLVNYNIALLQFDL
                     AKNEVFERYRIDVEAILDRVK"
     gene            6153..6557
                     /locus_tag="PLY93_02060"
     CDS             6153..6557
                     /locus_tag="PLY93_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRP68294.1"
                     /translation="MHSLVKYFRTMPPVALIGASNDSTKYGNIIFRDLKSKGFTIYPV
                     NPKATSIEGVPALHTLAELKEKTSVELLIYVIPPQLTLDSLKEAKELGYKKVWLQPGA
                     ADAGVAEFLDTNDFQYIIGACVMVESRDLVAS"
     gene            complement(6630..7022)
                     /locus_tag="PLY93_02065"
     CDS             complement(6630..7022)
                     /locus_tag="PLY93_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRP68295.1"
                     /translation="MFGLEKKATVENESEKTQNTAVLTPAVDIVETEQGYTIAADVPG
                     MDRENVDVSLENGVVTLKASRNSDKEEKIGEAIHREFRHTSYERSFRVSDDVDGEKIT
                     AEIANGVLRVNLPRKASSAKKTIAVTSK"
     gene            complement(7052..7498)
                     /locus_tag="PLY93_02070"
     CDS             complement(7052..7498)
                     /locus_tag="PLY93_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRP68296.1"
                     /translation="MLVKTHPLFHPTATQLWSDFAEAQSELDRLFRFNFSPREEGIHG
                     YYPPVNISENDDAYTVEARVPGLANENITVELEGRKLTIRGEHKREEANYTREERASG
                     QFERTITFKHALATDAIEAKVKHGILKVTLPKAAEAKPRKINVVTK"
     gene            complement(7741..8190)
                     /locus_tag="PLY93_02075"
     CDS             complement(7741..8190)
                     /locus_tag="PLY93_02075"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HRP68297.1"
                     /translation="MRITRTFLLLMAVLSVTLFAKDAPAAQHTLTVSVKGFRNSQGNF
                     IATLYNRDGSIPDEKFQKFYMQKKTAITNNASVIVFKNLPAGDYAVGILHDENSNGKI
                     DKGLLLPVEGVGFSNFNSIGLTNKPNFRKASFNLNSDKKISIKLIYF"
     gene            complement(8212..8928)
                     /locus_tag="PLY93_02080"
     CDS             complement(8212..8928)
                     /locus_tag="PLY93_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP68298.1"
                     /translation="MVNSGTGITITGLKKRYGSGETAVDALKGVNMHVSPGEVVGLIG
                     PSGSGKSTLLKCLGAVIEPTAGEMQLGSETIFRDKWIIPDITVLRRDKIGFVFQAPYL
                     IPFLDILDNVALLPMLAGVPNKEARKRAMELLDILDVAHRAKAAPRQLSGGEQQRVSI
                     ARALINRPPVVLADEPTAPLDSERALAVMRILNEMAGMYQTAIIVVTHDEKIIPYFKR
                     IYAIRDGETHEQKGEGRKFK"
     gene            complement(8921..>9232)
                     /locus_tag="PLY93_02085"
     CDS             complement(8921..>9232)
                     /locus_tag="PLY93_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRP68299.1"
                     /translation="VAFIIYTLTMGKIREIAVLKLIGTKNWTIASMILKQALVLGLIG
                     FAVGKIAVSFWAPAFPKYVLMLSSDTFIGFVLVMVICALASVIAIRIALKVDPAAAIS
                     G"
BASE COUNT         2447 a         2221 c         2006 g         2548 t
ORIGIN      
        1 ccccatcgcc acagaaagca atcccttggt gagtgcgttt tccagcggcc ccaaattgag
       61 tgcagtaaac gtgccacggt aggtaatctc cacttcacgg atagcgcgct tcatcatttg
      121 gccgatgaac gcccccactt gtgcgctcaa cgtaaaccat ggcgctaaaa tatccatcgc
      181 cgaagggtca atcgtcggga aattgagcga gttgcgcgcg agacccgatt tgaaaaatgt
      241 aaccatcgat tcggcggttt cttttgccac ctcgatttgc gcttcatcgg tcgatgcgcc
      301 taagtgcggc gtcatcacca cgttttcgag tgtataaaac tcgcttgttc cgggtggggg
      361 ctccactgta aacacatcga gcgcaacgcc gccaatcata ccttctttca ggcctttcac
      421 aagcgcgtcc tcgtcgtaaa taccgccgcg tgcacagttg ataaggcgga tgcccggttt
      481 taattgcgat aaattctttt cgttcacgag accttttgtc gtctctgtca atggcgtgtg
      541 caccgttacg aaatccgcct ctttgagaat tttatccaca gacatcagtt ggatttctaa
      601 gtgcgctaaa tgttccttcg ggatataagg atcaaacccc aacacttcca tttggagtcc
      661 cttcgcacgc tttaccactt ctttgccgat gcgccctaaa ccgataactc caagtttttt
      721 gccggtgagc tgcgtgcctt ggaatttgga tttctcccat tttttttctt tcattgaacc
      781 atgcgcttgc ggaattttac gcgcgaggga aaacataagc gcaatggctt gctcggcggt
      841 tgaaattgaa ttgcccgccg gcgcgttcat caccacaatc cctttttgcg aacacgcggg
      901 gatatcgatg ttgtcgacgc ccacgcctgc gcggatgaca actttgaggt tcgttgcttt
      961 ttcgacaaga gctttgtcga ctttcgatgc cgagcggatg ataaggccat cgacggtgcc
     1021 gacgacggct tcgagttctt cgcgtgtagt ttttttgcgg taatcgactt caaaaagccc
     1081 agaggcgttc aaaatatcca cgccgtcttt atcgagattg tctgcaatca aaattttagg
     1141 ttttaccata acttccccac ctgaaccgat aatgagtaca ccgcgtaaat aagagttaat
     1201 tttacaacta tcttggttta agagcctgta caccaactcg ctgaggcaat tttttcatac
     1261 acgctgcgca agttgacgca caggctctta agcgggcaat tctatctcaa aagcaaccaa
     1321 gtctgttttt tcatccgtgc catcgccaaa gtcttgaatt gttaaattac ggattgaacc
     1381 tttatgcatt tgaattattt tgtcgacgag agtgagcccc aacccgtatt ctaaagtgtc
     1441 gtacgcttcg ttcaaaattc gcgagacacg aaagaatggt tcaaaaataa tgcgacggta
     1501 ctcttcgggg atgcctttaa tttcgcctct attcactgtg tagggttcat tgagcacagt
     1561 gatcaaaatg cgggtgtgta agtattcaac caaaaccgtg atcgtcgatt ggggggacga
     1621 gaatttaagt gcattgatga ggagctcata gagcactttt cgcatgaact cccagttaag
     1681 cttaaggcga cgaccagcga accgagacgg taacgaactg acaacaatgc gatggccgcg
     1741 aatgtcagta aatggtttta tatcaaccac gatacttcga ataatatcgt gcagttcacc
     1801 caaggtaaca tcgaagaggg caaaattctg cgagactaat tcttgtagct caccgaaaac
     1861 accgaccatt ttattgagcg atttttcgtt ctctaaaatt aaatcgacga tatcgaccgg
     1921 agcaagataa tttttgccgt cggcgctctt aggtaacccc cgcagcatcg acagaagcgt
     1981 cgccaaagaa ccgatgcctt taccttgagt gaacgaagta taaatatttc caaaaagagc
     2041 gcgcgccact ttatcgtttt gcagcgacac gttgggtggc ttgctgcgtt tccagttgcc
     2101 gcgcgcgttc tttttcgagt gtgcgtaatc gcatgcattt atgcacttca tcaaaggcac
     2161 gctcaatagt ttttctcaat tcgttgggta caatcggttt aacgatgtag tcgtatgccc
     2221 ccaagcgtaa gcattcaatc acttgccgcg tgcctccttc gatggtttgc atcaccacta
     2281 caggtggaat ctcacgttca aaaagctgtt taagcacctc cacacccgaa tcgccgccct
     2341 tcaagtaaat atcgcacagc acgacggggt attgcatttt atcaaatgcg tctaacgcgt
     2401 ctttggtgtt gtcgacaaag gttaacgcgt acggcatttc attcaagatc gtttcaataa
     2461 gttcctgttg tactaaatcg tcttcgacaa taagtatcgg ctctcgatta gacatagtat
     2521 gagctttgtt tatagagacg atgcgtcaat ctatgaaaac gtaataataa gatctttgga
     2581 gtggttgatg gcggcgccag cagtcaatta gacaccacca tcatacaata tgaatgtcga
     2641 tttttttacc gttgtttagg ctattattca aaagctcaag ctcatactcg cctaaagtga
     2701 gttgggcaaa ccatgcaaaa tcttcgtgca acggtaacga ttgcagaagg cgtccgtcac
     2761 ggcgtaaatt gataaactgc gggcggggac ggtaattttg caatttcact gtgagcatca
     2821 cagtgtcttt gccgtctttc accacttgat agatcaactc accgctattg ttcgcttcgg
     2881 ttgtcatgaa ttgtaaagcg cctgttggcg caactgctgt gtcgattgct ccacgtactg
     2941 caaccatttg atctgtattt gctaagtcgt atagattgcg aacatggcct gcaataagac
     3001 gcagcccttc ttnnnnnnnn nncgcggcga ggtccatcgg ggcgagcacg aaccgatcgc
     3061 atcgaatgac gatggcgccg gtcgtgggtg caggcgccgc cgtttgaaga cggttcataa
     3121 taatttgttt caaatacgcg ggcgttggta tcaaaggttt ttcgaaccaa gtaaataaaa
     3181 aagaaagaat atcttgaaaa agcgagaaat ccgcatcgct tgttttaagc ttttgccaca
     3241 ccttgttttt agaaagcatt ttttccatga gatgggggtc atcgacgcta aaaaactgtt
     3301 cggcaatttc catcacctcg tgtgtggcaa gggcaaactt tgtcgcgcgt tgtgcagcgg
     3361 cgttgcgttt attgagcgca agcatcgttt caaactgcac aatcatatcg agcgacttga
     3421 ggtcgtcttg cgccaaagcg attgcctttg ctcgactttt ttcgtctaaa tacaatttca
     3481 ataaatgggc atattctttt gagccggatt taccctgtgt tttagtatct ttttgcatgt
     3541 ccctaatccc tactgacttt caaatactta gacgcgcaga aaacctcttt tacgacaaaa
     3601 tttttctcgt ttttaggcaa aaccctctaa attaagtatg ttcaagagcg tgaccgaaaa
     3661 cgatctaaaa atcctttcac agcttaaaaa aaacaacgat cgagaatggt tccataaaca
     3721 taagcccaaa attgaaattg cacaaaaaaa ctttctcgat ttagtaagtg ggttgatttt
     3781 tgtcctcagc gaatttgacg aatcggtatc caacgttgaa ccaaagagcg ccatttttcg
     3841 tatctatagg gatgtacgct atgcacacga taaatctcct tataaagcgc atttggcagc
     3901 gtttatttca gtaggtggcc gtttgagcga caccgaaccc ggttactatt tccacttcgc
     3961 cccccacggt gcatcgcttt tcggcgccgg cctctacatg tcccaaaaaa atacgctgcg
     4021 gaatgtacgc acagaaatcg cgcatcccca aagccggatc gtgcaaatgc tcgccgacaa
     4081 agttttccgt aaaagctttc ccgtcctcga cgaagacttg aagctcaagc gggtgccacg
     4141 cgggtttgac gccaaccacc cacaatgcga gctgttgaag ctgcgccacc actttgttta
     4201 cacccaactt gccgacaaag tagtcacttc aaaaaactta ctcaacaccc ttgcaaaagg
     4261 cgcagagcca ctttcgcgct ggtgtgccgt gttaagacaa gcgactcgag gttgaatact
     4321 ccagcttcca gtgtagtact caataattta aaaaagccta agttttatac aaaaaacact
     4381 cgcaaaatgc taaaacttat aacccattaa gtcatgcgga tagttatccc acattcttaa
     4441 ccaaatggtt aatttttttt gccctttatt ttcgttttaa ttatattatc gtggtggtat
     4501 ccatgaaaaa ttaccttgtt ttattctttg ttgcattcgc ttaccagccg tcgtttttag
     4561 cagcagaacc tactcccaaa acatccgatg aaaaggtaga aacaaaagct gagaaaagcg
     4621 acaaatcctc tgaaaataaa cccactgaag cgacgtttac gattgccggc acacagcttt
     4681 ccattcaaga cgctattaat atcgtactcg aaaaaaatct cactcttcaa gcggcaaaat
     4741 acgatgtgct gatgacagac acaaacgcca tgaagctaga aaaaaaatac gcaccaacgc
     4801 tttctggcga agtacagcat ttagatttta gcaactcccc tttagacgcc tttaacggca
     4861 actaccaaaa caacgcctac gtatctattt ccaaactatt tgctacgggt acaaccgtcg
     4921 gtggcggtta tcgctatcaa caactccata gtgaagcatc aaacggattt ttgggtctac
     4981 caattaaaac acccgagtca accaataatt ttcatggtta tttcattaac gtacagcaag
     5041 aattgctgcg caactcgttt ggctataacg atcggaaaat ggatcagatt gcgcgtaagc
     5101 aaagcgtggc acaacgcgcg tataccatca acttgctttc tggccttgtc gtgcaagcgc
     5161 tcaccgatta ttggcaagtg acgatccaaa agtttgcgct cgaaaacgcc aaactcgaag
     5221 aaaaatcaaa ccgccaagtg cgtgcgattg tcgctcgtaa cgtcaactat ggattgggcg
     5281 aaagttacga tttgaataac tataacgcgc gtgttgcaaa ctcggtggca aaggtcgcga
     5341 tgaccgaaca aagcctcaag aacgcaacgc gtaaactttt acgcacggta aacatgccag
     5401 tcgataccca aattgaaggc atcaccaatt tagtggatgt attgcccaac cttgatgcag
     5461 acgccgcact taaagcagcg atggaaaaac gagtcgactt aaaaaatgca aaaacagaac
     5521 tcgaagtcgc cgagttgcaa tccgatcttt atggtaacca agcattgcca tcgctcactg
     5581 catattttaa tttagtctcg aatggtacca acaacatgat tatgtttcca gggtttccgt
     5641 cggcaggtgc gctgcaaagc ccacaatggc aagtcggtgt tcgggcatcg tacccattgt
     5701 gggatcaaga aattaaagcg aataaacgca acgcaaacat gcaactctcg caagcccgca
     5761 taaaaatagc aaatgcagaa caagaaatcc gtgacgatgt attgacgcgc cttgagaatg
     5821 tacggctcac ccacgaaatt ttccaaaata gccgcacctc gcgcaaagaa tcggaagcgt
     5881 tttacaatcg tatgttggcg cgcactagaa ccggtaagct gaatttccaa ctcgtcggcc
     5941 aagcgttgga gaacatggtg gccaaccgcc agcgtgaatt ggagtcgctt gtgaactaca
     6001 acattgcgtt gttgcagttt gatttggcaa aaaacgaggt ttttgaacgt taccgtatcg
     6061 acgttgaagc gatactcgac cgtgttaagt aaatctttaa cgactaactt ctcgcaaact
     6121 tgacgcgcgg tttctttttg taaatttgcc ttatgcattc tctggtcaaa tactttagga
     6181 ccatgcctcc tgtggcactc attggcgcaa gtaacgattc aaccaaatat ggtaatatca
     6241 tctttagaga cttgaaatcc aaaggattta caatttatcc tgtaaatcct aaagccactt
     6301 ctatcgaagg agtgcctgct ctacacacgt tagccgagct taaagaaaaa acctccgtcg
     6361 aattgttgat ttatgtaata cctccccagt tgactctcga tagtcttaaa gaagcaaaag
     6421 agttgggata taaaaaagta tggttgcaac cgggcgccgc cgacgcaggt gtggctgagt
     6481 ttttagacac gaacgatttt cagtatatta ttggcgcgtg tgtaatggtg gaaagccgcg
     6541 acttagtggc atcgtaagcc actcgcgata aaaacatgaa ccttctaatt gccgcatacg
     6601 cggcaattag aaggtagatt aagctacttt tatttcgacg taactgcaat ggtttttttt
     6661 gcgctactgg ctttacgcgg taagtttacg cgtaatacac cattggcaat ttcggcggta
     6721 attttttcac cgtctacatc gtcgctgacg cggaacgacc gctcgtagct tgtgtggcgg
     6781 aattcacggt gtatcgcttc gcctattttc tcttctttat ctgagttgcg cgacgctttt
     6841 aacgtaacaa ctccgttttc tagagagaca tcaacgtttt cacgatccat ccccggcaca
     6901 tcggctgcga tggtgtagcc ttgttccgtt tctacaatat caaccgcagg cgtaagtacc
     6961 gctgtatttt gggtcttctc gctctcgttt tcaactgtcg ctttcttttc taatccaaac
     7021 ataaatcctc ctttagagca tggctctatt gttactttgt gaccacgttt attttgcgtg
     7081 gtttcgcttc ggctgctttc ggcagcgtta ctttcaaaat cccgtgttta actttagctt
     7141 caatcgcatc ggtcgctaac gcgtgcttga aagtgatcgt acgttcaaac tgtccgctgg
     7201 cgcgctcttc gcgggtgtag ttcgcttctt cgcgcttgtg ctcgccacgg atggtgagct
     7261 tgcggccttc gagttcgacg gtaatattct cgttcgccag tccaggaacg cgcgcttcga
     7321 cggtataagc atcgtcattt tcgctgatgt tcactggtgg gtagtaaccg tggatgcctt
     7381 cctcacgagg actaaaatta aagcgaaaga ggcgatctaa ttcgctttgc gcctcggcga
     7441 agtcgctcca aagctgcgta gcagtggggt ggaaaagtgg gtgtgttttt actagcatat
     7501 tgcctccatt gcgctaacac cataattatt gaaagtgtta gcggttgttt tatttgattg
     7561 atagcactca atcatcaaca ctgctaatat agtaaataca gactataccg gcaaattttt
     7621 tttacattat tttatacaat tttatgtgaa gccgacaccg taggctattt tgagtattcg
     7681 gtctggctgg tcatttgtgc tttgcgactt tcgacatacc ccaagaattt ctcttctccc
     7741 ttaaaaataa atcaacttaa tggaaatttt tttatcgcta tttaagttaa aacttgcttt
     7801 gcgaaaattg ggtttgttgg ttaggccaat cgaattaaaa ttggaaaaac cgacgccttc
     7861 caccggtaag agcaaacctt tatcgatttt gccgttactg ttttcatcgt gcaaaatgcc
     7921 gactgcgtaa tcccccgctg gtaaattttt aaagacgatt acggaagcgt tatttgtaat
     7981 cgctgttttt ttttgcatat aaaatttttg gaacttctca tcgggtatcg aaccgtcgcg
     8041 gttgtaaagc gtggcgataa aattgccctg cgaatttcta aaccccttga cgcttaccgt
     8101 tagggtatgt tgcgcggcgg gcgcgtcttt agcaaaaagc gtcacagaaa gcaccgccat
     8161 caaaagtaaa aatgttcttg ttatcctcat tgtaatctcc ttattatttt gttacttaaa
     8221 cttgcggcct tcgccttttt gttcgtgcgt ctcgccgtcg cgtatggcat agatacgttt
     8281 gaaatacggg ataatttttt catcgtgcgt gaccacgatg attgccgttt ggtacatacc
     8341 cgccatttcg ttcaaaatac gcatcaccgc caatgcccgc tcgctatcca acggcgcggt
     8401 gggctcgtcg gcgagtacaa ccggcggacg gttgataagc gcacgagcga tcgacacgcg
     8461 ctgctgttcg ccacccgaaa gttgacgtgg cgccgctttt gcgcggtggg caacgtctaa
     8521 aatatcaaga agctccatcg ctcgcttgcg tgcttccttg ttgggaactc ccgccaacat
     8581 cggcaaaagc gccacgttgt ctaaaatatc caaaaatggt ataagatatg gcgcttgaaa
     8641 aacaaaacct attttatccc gacgcaatac ggtgatgtcg gggatgatcc acttatcgcg
     8701 aaaaattgtt tcgctgccta actgcatctc acccgctgta ggttcaatca ctgcgcccaa
     8761 acatttaagg agtgtgctct ttcccgaacc cgaggggccg ataagtccca ccacttcacc
     8821 cgggctgacg tgcatattga cgcctttgag tgcatccacc gcagtttcgc ccgaaccata
     8881 ccttttttta aggccggtaa tggttatgcc tgtacctgaa ttaaccactg attgccgccg
     8941 ccggatcaac tttaagagcg atacgaatgg cgataacgct cgccaacgcg cagattacca
     9001 tcaccaaaac aaaaccgatg aacgtgtcgc tcgaaagcat gagcacgtat ttcgggaaag
     9061 ctggcgccca aaaactgact gcaattttac ccacagcaaa accaatcaga cccaacacca
     9121 acgcttgctt taatatcatc gaagcgatcg tccagttttt agtgccgatg agtttaagca
     9181 ccgcaatctc gcggattttc cccatggtga gagtgtagat aatgaatgcg ac
//
LOCUS       DAOMPD010000033         9162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3254_length_9162_cov_2.733062, whole genome shotgun sequence.
ACCESSION   DAOMPD010000033 DAOMPD010000000
VERSION     DAOMPD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_9162_cov_2.733062"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>954
                     /locus_tag="PLY93_02090"
     CDS             <1..>954
                     /locus_tag="PLY93_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRP68300.1"
                     /translation="VATPRLPVEHTLLGEAAQRDFRIINAIFARSHATAFKQIPAGEA
                     PELFHAKERVPLPVFLSRILKYYGNLHADHTMVGFPPELIEKYGLKNRFFPFPLKFFD
                     KHAYLDTQLSGVPYGAELVDINGKSIHSILQSFAEFPRARATDEPWTSFYLSEQFASW
                     YFLTSKSGAPWRLQFAGLPQETQLVTPNILPQRVSSVRNYWKSILSSMFMDKKKIAYF
                     AINSFFPTNTPYATFEKWVEYFGAFNTEARHRGSTTLILDLRVNRGGVMSLAAASAAW
                     FIETTIVDKSTSRVHTRLLPYREFATAINGQAAKNSDFDQL"
     assembly_gap    955..967
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <968..1422
                     /locus_tag="PLY93_02095"
     CDS             <968..1422
                     /locus_tag="PLY93_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68301.1"
                     /translation="LYRDFGDTMTDGYFETRNPLARHVKLVPLENVYRFQKIYVLIGP
                     QTYSAAVYFVRLLKLANSNVTLVGGETGSPGDGHSAETLITYRLPESGILFDVPLARV
                     EFAPLVPGQLPGKGLMPDKAIVPTLADFRAGRDTELEAVVDWVEKKSE"
     gene            1419..2987
                     /locus_tag="PLY93_02100"
     CDS             1419..2987
                     /locus_tag="PLY93_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRP68302.1"
                     /translation="MIRRYTKIAPAIFFLLVVFFLLQCKSSLIKEHPLSFSQWRVDET
                     IKYVQTHYGRTITSLEMLPVMVVAHYTAMDSTEVSFNYMNKEEMESGRSLLKKAGLNN
                     IAVHFLVANRHAIGIENVGKDESALTMAQVNANAYIVRELVKKYPIRYLIAHSEYRTF
                     ENTPLWEEKDKSYRTPKIDPGESFMQPLRAQLADLNLSAAYDGSEIPDRIDDTLKKYF
                     VRKEFEGSVLVIRHGKIIFQKIYGDIPPQKSFYLASAAKSITATLVHKLIAQKKIRLN
                     TPVVRYFPELKHLLKGVEVRHLLAHTSGLEDYYRLEGVEPGFTNTDVLRLLQTQKKPL
                     MKAGKKFHYSNSNYVLLALVVEKITGQKFEEVAHAKIFTPLKMIHTFFASETTRDKDK
                     ISAINAAEKYFDYPYKTTGAGGLYTTAEDMARFDEALLNNTLMLRPQFIAMLRPQARV
                     EKKNTEYAFGWYVYPQRGILYHDGNFNGYHTMNWLDIKNNSAIILLSSRQTQAIQDIT
                     FELDSILNGIAAEK"
     gene            complement(3033..3440)
                     /locus_tag="PLY93_02105"
     CDS             complement(3033..3440)
                     /locus_tag="PLY93_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HRP68303.1"
                     /translation="MKHTLWLIPLLVVFTFGCNKKKEFKGKVWFGEPLDKAELASPVK
                     FTMHVEGMEVKPAGTMQEGTGHFHILINKTAVPAGEVVITDPVHIHYGKGQTEDTRAL
                     EPGDYTVTLQFADGAHVSYGPAWAQSIHIKVKK"
     gene            3633..>5367
                     /locus_tag="PLY93_02110"
     CDS             3633..>5367
                     /locus_tag="PLY93_02110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP68304.1"
                     /translation="MLLEVDHPQFENCRSEISRFAELEKSPEYLHTYRITPLSLWNAA
                     ASRLKAEQIVDALYKYSKYPVPQAVIADIYTQMGRYGKLKLERDDRGDLVLKSTDARY
                     IAEVIRNKKVQPYIEGPKTPNEVYVKADYRGHIKQALIKIGFPVEDVAGYDSGNDYKF
                     ELRTKLPSGNDFVIRDYQDSSIRAFYADGSVEGGSGVVVLPCGAGKTIVGIGAMHAVG
                     AQTLILVTNTLSIRQWKNEILEKTTIPESDIGEYSGDKKEVKPITIATYNILTHRKKK
                     GGEFTHFHLFSSNNWGFIVYDEVHLLPAPVFRMTSELQAKRRLGLTATLVREDGLEED
                     VFSLIGPKKYDVPWKELEKKQWIAEARCVEIRTPLRSLMRGMYSVADDREKFRLSSEN
                     PEKLRVIGTLLYHHRNDQVLIIGQYLDQLQRVSARFKIPLITGKTPLDERARLYEAFR
                     TKQIRALVVSKVANFSIDLPDANVAIQISGTFGSRQEEAQRLGRVLRPKANGNQALFY
                     SVITNESSEEQFCRNRQLFLTEQGYEYYIYSYQDFRDKFGTYVEIMKKMKELREAEEN
                     NIAEAAPLKKKN"
     assembly_gap    5368..5388
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5389..6622
                     /locus_tag="PLY93_02115"
     CDS             <5389..6622
                     /locus_tag="PLY93_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68305.1"
                     /translation="YKKMILRFGIAIVVAAWLIWQIPFSSTHEIRLPELVTFLSFENN
                     TLAPEENPALTFYRRTEKGQYRTYGVSEAGKLVRTLDLPGHEIIRPDGTSIRMQDKEG
                     YITGPENGAYYIWYPQLGSQVYVFNEKGAFLWEKEESHYLHTLPRGRYILAAAGDQSR
                     MLFMNPDFKIHADFQGVLFNRFILDDNPDLKSAQICLGTLDGDVIVAHLDKKLYFRKK
                     LGYALKSLQCDFSTSTLAAIIEERPISESKDKGAVAKDILLRAKFQLDPAEKQNTAET
                     MTAIKANIEIQSQIDLPALTQTASPMALTENTTCFLQAEGETVQIFYTPNRRAKIVKI
                     SLSVKNKQELSADTWRSARITVDTMQSCLFAHPSGHWLLANERGLLAERFDLPSIRLL
                     NNKSAVFMQTPTGIFKLQ"
     gene            complement(6641..8074)
                     /locus_tag="PLY93_02120"
     CDS             complement(6641..8074)
                     /locus_tag="PLY93_02120"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRP68306.1"
                     /translation="MIQRLMTFLQVPSITKPTTSLEKDYFFPKRLPEHLDVIFFEEYI
                     RTLRIPLVWGTVLFFGIVLLVICYYASNFAYFREWIYIVTFATLLTFVELYWGWEVTR
                     RFGYRNQLTILNIVLAFLVSTISWGIIGAWQTMSQSYIGMMTTIIGSILGAGAVILAY
                     SRVSYMTFMLGWIIPGLVFMFSKSDPFFLFMGILFTGGIFSFTFINIMEHAKQRQLFD
                     ANHQLTEINNELVRKENEIDQELLFASHIQMGIFPEPKTQIPYWKFQTTVLPLGRISG
                     DYCDIVTRDNVTFVIMADASGHGVPAALLTMAAKNVFANTLIERISPAFALAKCNREI
                     LKMIKTQDYMTGFLLKMYPSGLVEFSNAAHPTAFHLHSDGRVKALDTSGFILGGLEDV
                     DALYENGSKQLLPEERLFLYTDGVTESTNHQNKFYGVERLEKLLQKSATFEASKIIPT
                     VFSDVVGFCEGEPFRDDIAMMVIEYAP"
     gene            8135..8374
                     /locus_tag="PLY93_02125"
     CDS             8135..8374
                     /locus_tag="PLY93_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68307.1"
                     /translation="MKDIEIAKILYLAKSKKYEVACASFDVVDHLGKLDVPKNLKRRK
                     NAVQTLSLLADEQVKFGYERRAEVALADDDEDETT"
     gene            8437..>8928
                     /locus_tag="PLY93_02130"
     CDS             8437..>8928
                     /locus_tag="PLY93_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRP68308.1"
                     /translation="MVGTGRYPDLLTMQRCHEVAETQMVTVALRRIDLTAAKTPQGKS
                     TAIIDYIDRKKITLLPNTAGVSFAADALRYARIAAAMDCTHLKVEVTGDLTTLLPDPI
                     ETLKAVELMRGHEDTRSLYLMVYTNDDPVLARRLADAGADCIMPAASPIGSGRGIANE
                     ANLI"
     assembly_gap    8929..8938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8939..>9162
                     /locus_tag="PLY93_02135"
     CDS             <8939..>9162
                     /locus_tag="PLY93_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRP68309.1"
                     /translation="LLETMHGRAPVIVDAGIGLPSHATRAMELGADAVLLNTAIAKAK
                     EPVIMAAAMRDAVKAGRAGFLTGAIPPKLY"
BASE COUNT         2574 a         2241 c         2072 g         2231 t
ORIGIN      
        1 agttgcaacg ccgcgtctac cggtagaaca cacgctttta ggcgaggcag cacagcgcga
       61 ctttcgtatc ataaacgcca tttttgcccg ctcacacgcc accgcgttta aacaaatccc
      121 cgcaggcgag gcgcccgaac ttttccacgc taaagaacgt gtaccactac ctgttttcct
      181 ctcgcggatt ttgaaatact acggcaatct acacgccgac cacaccatgg tgggattccc
      241 tccggagctc atagaaaagt acggattaaa aaatcgattc tttcctttcc cattaaaatt
      301 ttttgataag catgcgtacc tcgatactca gttgagtggt gtgccgtatg gcgcagaact
      361 tgtggacata aatgggaaat ccatccacag tattttacag tcattcgctg aatttccacg
      421 ggcgcgcgct accgacgagc cgtggacatc tttttatttg agcgaacaat ttgcgagttg
      481 gtatttttta acgagtaaga gcggtgcacc ctggcgctta caatttgcgg gtttgccgca
      541 agagacacaa ctcgttacgc ccaacatatt accgcagcgg gtaagtagtg ttcgtaacta
      601 ttggaaatcg attttatctt cgatgttcat ggataaaaaa aagatcgcat acttcgctat
      661 aaactcattt ttccccacca acacaccgta tgcaacgttt gaaaaatggg tggagtattt
      721 cggcgcgttc aacaccgaag cgcgccatcg tggctccacg acactcattc tagatttgcg
      781 tgtgaaccgt ggcggcgtga tgtcgttggc ggcggcgtcg gcagcgtggt tcattgaaac
      841 taccattgtc gataaaagta ccagccgcgt acacacacgc cttctaccct accgcgagtt
      901 cgcgaccgcg attaacggcc aggccgccaa gaactctgac tttgatcaat tagannnnnn
      961 nnnnnnnttc tttaccggga ttttggcgac accatgacgg atggctactt tgaaacgcgt
     1021 aacccgttgg cgcgccacgt taaacttgtg ccactggaaa atgtgtaccg ttttcaaaaa
     1081 atctatgtac tcatcgggcc gcaaacgtat tcggcggcag tctattttgt gcgtcttttg
     1141 aaattagcca actccaacgt cactctcgtg gggggcgaaa ccgggagtcc tggcgatggg
     1201 catagcgcag aaacgctcat cacataccgt ctgcccgaat cagggattct tttcgatgtg
     1261 ccgttggcgc gtgtagaatt tgcaccgctc gttcctgggc aattgccggg caaaggcctt
     1321 atgccggaca aagccattgt gcccacactc gctgattttc gtgcggggcg cgacacggaa
     1381 cttgaagcag ttgtggattg ggtagaaaaa aaatcagaat gatacggcgc tacacaaaga
     1441 ttgcacctgc aatttttttt cttttagtcg ttttttttct attgcagtgt aaatcatcct
     1501 tgattaaaga acacccgtta tcgttttcgc agtggcgtgt ggacgaaacg attaagtatg
     1561 ttcaaacgca ttatggccgc accatcactt cgctcgaaat gctgccggtg atggtggtcg
     1621 cacactacac cgcgatggac tctaccgaag tttcgtttaa ctacatgaac aaagaggaga
     1681 tggaatcggg gcgctcgctt ttaaaaaagg cgggcctcaa caatatcgct gtgcattttt
     1741 tggtcgcaaa ccgccatgcg attgggattg aaaacgtggg taaagatgaa agcgcgctca
     1801 ccatggcgca agtaaatgcc aatgcgtata ttgtgcgtga gcttgtgaaa aaatatccga
     1861 ttcgttattt aatcgctcac tcagagtacc gcacgtttga aaatacaccg ctctgggaag
     1921 aaaaggataa atcgtaccgc accccaaaaa ttgacccggg agaatctttc atgcagccat
     1981 tgcgcgcgca acttgcagac ttaaacttaa gcgcggcgta cgatggcagc gaaatccccg
     2041 accgcatcga cgacactctc aaaaaatatt ttgtgcgtaa agaatttgaa ggtagcgttc
     2101 ttgtcatacg ccacggtaaa attatttttc aaaaaatcta tggcgacatt ccgccgcaaa
     2161 aatcgttcta tcttgcatca gcggcaaagt ctatcaccgc aacgctcgtg cataagctta
     2221 tcgcacaaaa gaaaatacgc ctcaacacac ccgttgttcg ctattttccc gaactcaaac
     2281 atctcctcaa gggcgtagaa gtgcggcacc tcttggcgca cacctctggc ttggaagatt
     2341 actaccgtct cgagggagtg gagcccgggt ttaccaacac tgacgtgctg cgcctgttgc
     2401 aaacgcaaaa gaaaccgctt atgaaagcag gaaaaaaatt tcactactct aactcaaact
     2461 acgttctttt agcgctcgtt gttgaaaaaa tcacagggca aaaatttgaa gaagtggcgc
     2521 acgcaaaaat ttttacacct ttaaaaatga tacacacctt ttttgcttcg gagactacgc
     2581 gtgacaaaga taaaatatca gctattaacg ccgcagaaaa atactttgac tatccgtaca
     2641 aaacaaccgg tgcgggggga ctctatacca ctgctgaaga tatggcgcgc ttcgacgaag
     2701 cgcttctcaa caatacattg atgctgcgcc cacagtttat agctatgcta cgcccccaag
     2761 cacgcgtaga gaagaaaaat acggagtacg cattcggttg gtacgtctat ccccagcgcg
     2821 gaatcctata ccacgacggc aacttcaacg gctatcacac gatgaattgg ctggacatca
     2881 aaaacaacag cgcgattatt ttactcagca gccgccaaac gcaggctatc caagacatta
     2941 catttgagct ggatagtatt ttaaacggga tagcggcaga aaaataaacg cgctcaatga
     3001 atttggaatg ccgcgcgtgt atgtatcctg tattattttt ttactttaat atgaatcgac
     3061 tgtgcccatg caggaccgta cgagacgtgc gcaccatcgg cgaattgcaa tgtcacggta
     3121 tagtcgccgg gctctaacgc gcgggtgtct tcggtttggc ccttaccata gtggatgtgt
     3181 acgggatcgg tgattacaac ttcacccgca ggcaccgcgg ttttgttgat gagaatgtgg
     3241 aaatggccgg taccttcttg cattgtaccc gccggtttaa cttccatacc ttcgacgtgc
     3301 atggtgaatt tcacaggcga cgcaagctca gctttatcta aaggttcgcc gaaccaaact
     3361 ttgcctttga actctttttt cttattgcaa ccgaaagtaa aaacaaccaa aagtgggatt
     3421 aaccaaagtg tgtgtttcat accccacagt gaaaggctat tactcttgta aagggatttg
     3481 ttatttgatt ttatcaatcc aaagcgcaac ttctttagta tattgtcctt tacacggcgt
     3541 ctttgtcttc gatgccatgg cgcccgaaat agcaaggaga aaacttgagc agtttagacg
     3601 aaaaaccact aattgtccaa tccgataaaa cattgttact tgaggtcgat catcctcagt
     3661 ttgaaaattg ccgcagtgaa atttcacgtt ttgcagaatt agaaaaatca cccgagtact
     3721 tgcatactta ccgcatcacg ccgttatctt tatggaacgc cgctgcgtcg cgtcttaaag
     3781 cagagcaaat tgtcgatgct ctttataaat actccaaata ccctgtaccg caagcagtga
     3841 ttgccgatat ttacactcaa atggggcgtt atggcaaact caagttagag cgcgacgacc
     3901 gtggcgacct tgtacttaaa tcgacggatg cacgctatat cgccgaggtg attcggaata
     3961 aaaaagtaca gccttacatc gaaggtccta agacgcccaa cgaagtgtat gtaaaggcag
     4021 attaccgagg ccacatcaaa caagcgctca ttaaaattgg ctttcccgta gaagatgtcg
     4081 cgggttatga cagcggcaac gattacaagt ttgagttacg cacaaaactc cccagcggta
     4141 acgatttcgt aatccgcgat taccaagatt cgtcgatccg cgcattttac gccgacggca
     4201 gcgtcgaagg cggttcgggg gttgtggtat tgccttgcgg cgccggcaaa acgattgttg
     4261 gcatcggtgc aatgcacgca gtcggtgcgc aaacgctgat tttagttacc aacacgcttt
     4321 ccatccgcca atggaaaaat gaaattttag agaaaacaac aatccccgaa agcgacatcg
     4381 gcgaatactc aggcgataaa aaagaagtta agcccatcac cattgcgacg tacaacattc
     4441 tcacgcatcg taaaaagaaa ggcggagagt tcacccactt ccatttattc tcatcgaaca
     4501 actggggatt tattgtgtac gatgaagtac atcttttgcc agcgccggtt ttccgtatga
     4561 cgagtgaatt acaagcaaag cgacgcttgg gcctcactgc aacgttggtg cgcgaggacg
     4621 gtctcgaaga ggatgtgttc tcgctcattg gtcccaaaaa atacgatgtg ccatggaaag
     4681 agctggaaaa gaagcagtgg attgccgagg cgcgctgtgt agaaatccgt acgccgttgc
     4741 gctcgctcat gcgcggcatg tattcggttg ccgacgaccg cgagaaattc cgcctctcct
     4801 cggaaaatcc agaaaaatta cgcgtcatcg gtactttact ttaccatcac cgcaacgacc
     4861 aagtgctcat cattggccaa tacctcgatc aactacaacg agtttcagcg cgttttaaga
     4921 tacctttgat taccggtaaa acacctctcg acgaacgtgc gcgactttac gaagcgttcc
     4981 gtacaaaaca aatccgcgcg ttggtcgtct caaaagtggc aaacttttca atcgacttac
     5041 ccgacgccaa cgtggccatc caaatttccg ggacgttcgg cagtcgccaa gaagaagcac
     5101 agcgtttggg acgggtttta cgccccaaag caaacggcaa ccaagcgctt ttttattcgg
     5161 tgatcaccaa cgaatcgagc gaagaacagt tttgccgcaa ccgccagctt tttctcaccg
     5221 agcaagggta tgaatattac atttactcat accaagattt ccgcgataag ttcggtacat
     5281 acgtggagat tatgaaaaaa atgaaagaac tccgcgaggc agaagagaat aacattgctg
     5341 aagcagcgcc tctcaagaaa aaaaaccnnn nnnnnnnnnn nnnnnnnntt acaaaaaaat
     5401 gattctccgc tttggcatag cgatagtcgt tgcggcgtgg cttatttggc aaataccatt
     5461 ttcgtcgacg catgaaattc gtttacccga attagttaca tttttatctt ttgaaaacaa
     5521 cacacttgct ccagaagaaa atcccgcatt aacgttttat cgtcgtacgg aaaagggcca
     5581 ataccgcacg tatggcgtca gcgaagctgg caaacttgta cgcacgctcg atttacccgg
     5641 ccacgaaatt attaggcccg acggcacgtc gattcgcatg caagataaag agggatatat
     5701 cactggccct gaaaacggcg cgtattatat ttggtaccca cagttaggct cgcaggttta
     5761 tgtttttaat gaaaaagggg ctttcctttg ggagaaagaa gagagccatt atttacacac
     5821 tctaccgcgc gggcggtata ttttagccgc tgcgggcgat caatcgcgta tgctctttat
     5881 gaaccccgat ttcaaaatcc atgcagattt tcagggagtc cttttcaacc gttttattct
     5941 cgacgacaat cccgacttaa aatctgcgca aatttgttta ggcacgctcg acggcgatgt
     6001 cattgtcgcg cacttggaca aaaaacttta ttttcgtaaa aaattgggct atgcattgaa
     6061 gtcgctgcaa tgcgattttt ctacatcgac gctcgctgcc atcatcgaag aacgcccgat
     6121 aagtgagtca aaggataaag gagctgttgc aaaagatata ctgctccgcg caaaatttca
     6181 acttgatccc gcagagaaac aaaacacggc agagacgatg acagcgataa aggcaaatat
     6241 cgagattcaa tcccaaattg atttacccgc actcacgcaa accgcctcac ctatggcgct
     6301 gacagagaac acaacgtgtt ttttgcaagc agagggtgaa acggtgcaaa ttttttatac
     6361 gcccaaccga cgagctaaaa ttgtaaaaat ttccctcagc gtcaaaaata agcaagaact
     6421 ctctgccgat acatggcgta gcgcgcgcat aaccgttgac acaatgcaaa gttgcctttt
     6481 cgcacaccca tcggggcatt ggcttcttgc caacgagcgg ggtttgttag ccgaacgctt
     6541 cgacttaccg agcatccgtt tattgaacaa taaaagcgcc gtgtttatgc aaacaccgac
     6601 gggaattttt aagctacaat aaataaattc gaatacaagc ctacggcgcg tattcgataa
     6661 ccatcatcgc gatatcgtca cggaagggtt cgccttcgca aaaccccaca acatctgaaa
     6721 acaccgttgg aataattttg ctggcttcaa aagttgccga tttctgcaat aatttctcaa
     6781 gacgttccac tccgtaaaat ttattttgat gatttgtact ttcggtaacg ccatcggtat
     6841 aaagaaaaag tcgttcctcc ggcaataact gcttactacc attttcgtag agcgcatcga
     6901 cgtcttcaag tccacccaaa ataaaccccg atgtatcgag tgctttgaca cggccatcag
     6961 agtgcaaatg aaacgccgtt ggatgcgctg cattagaaaa ctccactaat cccgatggat
     7021 acatttttag taagaagccc gtcatatagt cttgagtttt gatcattttc aagatttcac
     7081 ggttacattt tgcgagtgca aacgcaggag agatacgctc gattaaagtg ttggcaaaga
     7141 cattcttcgc ggccatagtg agtagcgcgg caggcacacc atgccctgac gcgtcggcca
     7201 taatcacaaa cgttacattg tcacgcgtta caatgtcgca gtagtcgcct gagatgcgcc
     7261 ccaacggcag cactgttgtt tgaaacttcc aataaggaat ttgagttttg ggttcgggga
     7321 aaatacccat ttgtatgtgc gaggcaaaaa gtaattcttg gtcgatttca ttttctttgc
     7381 gcactaactc gttatttatt tcggtgagtt gatggttagc gtcgaaaagc tgtcgctgtt
     7441 tcgcatgttc catgatgtta ataaatgtga atgaaaaaat accacccgta aaaagaatcc
     7501 ccataaaaag aaaaaaggga tcgcttttag agaacataaa aacaagcccg gggataatcc
     7561 aaccgagcat aaaagtcata taactcacgc gtgagtaggc gagaatcacc gcgcctgctc
     7621 ccaaaattga accgatgatg gtcgtcatca tgccgatata gctttggctc atcgtttgcc
     7681 atgcaccaat gatgccccat gaaatggtac tgactaaaaa cgctaaaaca atgtttaaga
     7741 tcgtgagttg attgcgataa ccaaagcgac gcgttacttc ccacccccaa taaagctcga
     7801 caaaagttag gagtgtcgcg aatgttacaa tgtaaatcca ctcgcggaaa taagcaaaat
     7861 tcgacgcgta gtaacagata actaaaagta caatgccaaa aaaaagaacg gtaccccaaa
     7921 ccaatggaat acgtaatgtg cggatatact cttcaaaaaa aataacatcg agatgttcgg
     7981 gcaaacgctt gggaaaaaaa tagtcttttt cgagcgaggt ggttggctta gttatagacg
     8041 gtacctgcaa aaaagtcatg agtcgttgga tcaaaacatt cgctccattt tagtttacac
     8101 gctgtgtcgt tgaattaggc tgtcaatcga ctccatgaaa gacatcgaga ttgcaaaaat
     8161 cctctacctc gcgaaaagca aaaaatacga agtggcgtgc gcgagctttg atgttgtaga
     8221 ccacttaggt aagttagacg ttcctaaaaa tctcaaacgc cgcaaaaatg cagttcaaac
     8281 gctgagtctt ttggccgacg aacaagtaaa attcggttac gaaagacgtg ccgaggttgc
     8341 gctcgccgac gacgacgaag acgaaaccac ttaacgacgg cggtagccac gaactctcct
     8401 cttacactcg gtacccttca actcacctcg cgccttattg tgggcacagg gagatacccc
     8461 gatttattaa ctatgcaacg ctgccatgaa gtggcagaaa cgcagatggt caccgtcgcg
     8521 ctacgtcgca tcgatttaac agcggcaaag acaccacaag gaaaatccac cgcaattatc
     8581 gactacatcg accgtaaaaa aatcacactc ttgcccaaca ccgctggcgt ttcgtttgcc
     8641 gccgacgcgt tgcgttacgc acgcattgcc gccgcgatgg attgcacgca cttgaaagtt
     8701 gaggtgacag gcgacctcac aacgctcctg ccagacccca ttgaaacctt aaaggcggtt
     8761 gagctcatgc gtggacacga agacacccgc tcgctctact tgatggttta caccaacgac
     8821 gatcccgtgc tcgcccgccg ccttgccgat gctggcgccg attgcattat gcccgccgca
     8881 agccccattg gttcgggtag ggggattgcc aacgaggcca acttgatann nnnnnnnntc
     8941 tcttggaaac aatgcatggc cgcgcccctg ttattgtcga tgcaggtatt ggtttgccgt
     9001 cgcacgccac acgcgccatg gagttggggg cggatgcggt gctcctcaac acagccatcg
     9061 caaaagcaaa ggagccggtc atcatggccg ccgcgatgcg cgatgcggtc aaagcaggtc
     9121 gtgcaggttt tttaaccggc gcgatacccc ccaaactata tg
//
LOCUS       DAOMPD010000034         9149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3261_length_9149_cov_2.563338, whole genome shotgun sequence.
ACCESSION   DAOMPD010000034 DAOMPD010000000
VERSION     DAOMPD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3261_length_9149_cov_2.563338"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLY93_02140"
     CDS             complement(<1..313)
                     /locus_tag="PLY93_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRP68310.1"
                     /translation="MSIEEREASLVENFETFDDWADRYRYLIDLGEELPTLDEAHKIA
                     ENKVQGCQSQVWLIARREGDKILFEADSDSAITRGLIALLVQLFSGATASEIASADLS
                     FI"
     gene            complement(447..3563)
                     /locus_tag="PLY93_02145"
     CDS             complement(447..3563)
                     /locus_tag="PLY93_02145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68311.1"
                     /translation="MDFLRSVYLDYYTIGAMIPVVFLFIAATIFYQIKDKSRATKALA
                     DGMLIYGFFVLSYVVASTLYHPYAAFHRWNTVLCVLWVLAVFVRVFFEYPEPRNTKFG
                     KILFYSLLILTAGFAAWFVTQTIHANFVYHFEGHYYDFDADVASYVISLAIIASFVLL
                     IAVGVWRAVLAKGRDRWSVIGMIFGFTCGALVPAALNTLSREGGMDRGTFQTAFDLFT
                     ILGLFVIVIIYLNSTKDKTTFMAKIILVCVVVFMTVVQAISYFSFQDKELAYDGVKAE
                     QTNRFRETGTEPESMRYAVAYDLVSKKLEALKSLNGSEPKTEEHINGFEFSYVWQKIL
                     FLKEDIAFKDSIERILRDAPATFGGYTKSLLPLLDVKETANKSAILHKIQDLQRPVIH
                     QRYKINKLPNFHFREKVTALLLKAPSKVRPFADAMLAYLKENTVDNGAELKSKVLQYI
                     EEVIPPGARHYRVDKKSNAHYVAYVSIDFSKNRVVEVGYDYLNYRKFVHPLALKFLIL
                     LVIAVVLIVVGFPIFFSRALVTPLNNLLSGVNQVNTGNLDIRIPIYVEDEIGYLSHSF
                     NGMVASIRDSKQKLQEYATTLEDKVKERTAELQKAFEDVTALKKQQDGDYFLTSLLTT
                     PLSSNTAESPTLNVDVLLEQKKKFEFRKWSRDIGGDTCITGTIILKDKPYTVFLNADA
                     MGKSMQGAGGILVLGSVFHAVVERTKVSPKEKDVFPEQWLKHTFLELHRAFEVFDGSM
                     LVSLIMGLVDDQTGLLYYINAEHPWSVLYREGKAEFIEKELVLRKLGTLGVSGHIEVM
                     TLQLEPKDVVIMGSDGRDDLLLGYESDGTRIINEDELLFLKVVEDSNGQLDRIRENLE
                     NYGELTDDLSLLRIGYQEGVHHDNTQLSQHVIQLVNRARSADKAGQLKVAIADLEEAN
                     RMDDSQPTVMRDLVKAYLKAKEYEKAAALAEDYIHIKPGDSEFLYLVSYCYKILGDYK
                     KAADFGERLYLRHHDMVKNLVNLADTYFQQKNYNRSRSFIDLAAKLDPHNIKVKRLIE
                     SLRRVEST"
     gene            3666..5015
                     /locus_tag="PLY93_02150"
     CDS             3666..5015
                     /locus_tag="PLY93_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRP68312.1"
                     /translation="MEIIHIIGAGGVGMSALALLAKRRGATITASDKNNSSYLQKLAS
                     HGIHTWVGSQPEKIPQDAHVYYSSAVKADDPERDFAETHGFLCEPRHSLLKRITADYF
                     TIAIAGCHGKTTTSAWLADLLIRAGKDPTALIGGTVPEWQSNYREGSGKLHGKPILVM
                     EADESDRSFLSIDVDIALITNVDLDHTDIHQSLAALENDFCAFADTARQNNGNIFLSS
                     ECDGALLARLTPAEKEARAQVIIAMEKHSIAAQGMEYPVGLAGVHNLKNATLIVQLGL
                     TLGIPPTVIAASLQEFSGVNRRMQRIATFPERHLTVIDDYAHHPHEVEATLAALEPQY
                     DRLLIFWEPHRLSRFVHFYREFAAVLEKYAAEHQLFLLPIYASGHKAEDYPHYEKLFQ
                     QFTKSHFVYIAQPSEFSLEPLALDGRNTAAVFMGAGASSEYAQAFVEFVRQQLTQKR"
     gene            5046..7439
                     /locus_tag="PLY93_02155"
     CDS             5046..7439
                     /locus_tag="PLY93_02155"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRP68313.1"
                     /translation="MLPLQTDLKTELKEGFHRHLNFSLGRTLDELTNEDAYISLSLSL
                     RDLMMRRLMKKRQRMRQKKRVYYLSMEFLMGRMLQVALVNMEMTTIAKEVLSEMKINL
                     DELYAIEPDAALGNGGLGRVAACVLDSMAHYDFSCIGAGLCYQFGLFEQSFHDGVQYE
                     SPDVWLTGNYPWLIDRHDKRYDIHFYGHVERQGARATWRHGDSVVAMARDMLIPGNGS
                     EGIATLRLWWAEHGSDFGLDYFQHGDYLKAMERQIYSESISKVLYPNDNNLSGQELRL
                     KQEYFLSSATIQDALSTFKFEGGNFSLLADRVFFQLNDTHPAIAIAELMRILVDREGI
                     EWGKAWSITQKCMGYTNHTIMPEALEKWNLDLFGRVLPRHLEIIYDINFRFLDELRAK
                     GFDQDAIGRASIIEEGHPKRVRMAALSVVASSAVNGVSALHSNLVKTRLFPDYAKLWP
                     EKFSNKTNGIAFRRWLIAANPNLANLIDNTIGDKWRSEHSLLAALATHKDDAALHNHW
                     KKIRTDNKKLLAAVIKERTGITIPTDSFFDIQVKRIHEYKRQLLNLMRVARCCLDILA
                     GTYTIETHRTFIFAGKAAPGYHRAKLMIRLANALSAYIEKNPLIKKRIQLVFLPNYAV
                     SLAERIFPAADLSEQISTAGTEASGTGNMKFMLNGVVTLGTLDGANVEIREEVGDANI
                     YIFGHTVETLNALQETGYNPVEFAKNPRLAEIFEMFEILHGDFREFFDALTYQGDPYF
                     HLADFKNYWEVQETIMTDYLNSASWLSKEIMNTAHSGKFSADRTIYEYARDIWKIVE"
     gene            7442..>9149
                     /locus_tag="PLY93_02160"
     CDS             7442..>9149
                     /locus_tag="PLY93_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRP68314.1"
                     /translation="MLFPVTGFPVPKNFQKSIPTHAGRIHLTSFQDAEQSAWRISKKT
                     DALFLTKAAHLSKLLYRILKKVSIQPSMSSEQYLSAAHSFAIPEASVGFPFCGLYFLS
                     IHNPTLLPLHPTLVVEKSAAVIGALDGDTTLERLLKLLNDNPTSIDAQIQALLKAHAD
                     FFTDEEKSHFLQIVHYTAGAKTQANAAPLYGYDAPVGAPQLRSHDKAWMQSLVLIAKI
                     VPVWLVDIRRRYGIDARRLDDVPDAELVHLKSFGVNGIWLVGLWQRSAASARIKNLSG
                     GKNYASAYSIFDYRVADEWGGEDALEQFYHRCARHGIRLGCDMVPNHTGIDSKWLREN
                     PDWFMQVEESPFPNYRFSGENLSADAPMEIRIEDGYYTHRDAAVVFEYKKNNRTRYIY
                     HGNDGTGLPWNDTAQLDHTRADVRQVIIDTALSLTRYFSVIRFDAAMTLTREHYHRLW
                     FPPPSAPPAIPSRSAATLSAQDFSAQMPREFWADLVEAFRKKSPETLLIAEAFWMTEM
                     FFVRNLGMHRVYNSAFMHALRDEKNHEFQKTLTTFREHDEGTLTRFVNYLSTPDEEAA
                     RAH"
BASE COUNT         2398 a         2312 c         2151 g         2288 t
ORIGIN      
        1 cgataaacga taaatcggcc gatgcaattt ccgatgccgt tgcaccggaa aaaagttgga
       61 ccaaaagcgc aatgaggccg cgggtaatcg ccgaatcgct gtcggcttca aagaggattt
      121 tatctccttc gcgacgcgcg atcaaccaca cttgcgattg acatccctgc actttgtttt
      181 cggcaatttt atgcgcctcg tctaaagttg gcaattcttc gcccaaatcg ataagatatc
      241 tatagcgatc cgcccaatcg tcgaaggtct caaaattttc tacaagactt gcttctctct
      301 cttcaatcga catgcgacca aaccaaaatt atgcgacgca ctgtaagcta agagttgacc
      361 tgcctacgag cctttgacca actcgcgcag cggatacaaa gaggcaattt tttgcaaatt
      421 tttgtacgtg gttggcgcac aggctcctac gttgattcta ctcgccgcaa cgattcgatt
      481 aaacgcttca ccttaatatt gtgcggatcg agctttgccg ccaagtcgat aaagcttctc
      541 gaacggttat aatttttctg ttgaaagtat gtatccgcca gattcactaa gtttttcacc
      601 atgtcgtggt gtcttagata aagccgttcg ccaaagtcgg ccgctttttt atagtcaccc
      661 aaaattttat agcaatacga aactaaatag agaaattccg aatcaccggg cttgatgtgg
      721 atgtagtcct cggcaagcgc cgccgctttt tcatactctt ttgctttgag gtatgctttc
      781 acaaggtcgc gcatcaccgt tggttgcgaa tcgtccatac ggtttgcttc ttcaaggtcg
      841 gcgatggcaa ccttgagttg gcccgctttg tccgccgagc gcgcacggtt caccaactgg
      901 attacatgct gcgaaagttg ggtgttgtcg tggtgcaccc cttcttgata accgatacgc
      961 aacaaggaaa ggtcgtcggt gagctcgccg taattttcta aattttcacg aatgcgatcg
     1021 agctgaccat tgctatcttc gacgactttc aagaaaagta gttcgtcttc gttgatgata
     1081 cgcgtgccat cgctttcata acccaataat aaatcgtcgc gtccgtccga ccccataatg
     1141 acgacgtctt tgggttccag ttgtaacgtc atcacttcga tgtgaccgct tacgcccaac
     1201 gtacccaatt tacgcagcac gagttctttt tcaataaact ctgctttacc ttctcggtag
     1261 agcaccgacc agggatgttc tgcgttgatg taatacaaca aaccggtctg atcgtcaacg
     1321 agtcccataa ttaacgaaac gagcatcgaa ccgtcgaaca cttcaaaagc gcggtgtaat
     1381 tcaaggaatg tatgtttcaa ccactgttcg ggaaacacgt ctttttcttt aggagaaact
     1441 ttcgtgcgtt cgacaacggc gtggaacacc gaacctaaaa cgagaatccc ccccgcgcct
     1501 tgcatcgact tacccattgc atcggcgttg aggaaaacgg tgtaaggttt atctttaaga
     1561 ataatcgttc ctgtaatgca ggtgtcgccg ccaatgtcgc gcgaccactt gcggaactca
     1621 aattttttct tttgttctaa aagcacgtcg acgtttagcg ttgggctttc agcggtgttt
     1681 gaactgagcg gtgtcgttaa aagactcgtt aaaaagtaat ccccatcttg ttgtttcttg
     1741 agtgcggtta catcttcaaa cgctttttgt aactcggcgg tacgctcctt cactttgtct
     1801 tccaacgtgg tggcgtactc ttgcaatttt tgttttgagt cgcgaatcga ggcaaccatg
     1861 ccgttaaacg aatgcgataa atagccaatt tcgtcttcga cataaattgg aatgcggatg
     1921 tccaaattgc cggtgttgac ttgattaacc cccgagagaa gattattgag cggagtcaca
     1981 agggcacggc taaaaaagat ggggaagcca accacgatta aaacgacggc gataacgagg
     2041 aggatcaaaa attttaacgc aagcggatgc acaaactttc gatagttgag ataatcgtac
     2101 cctacttcga caacgcggtt tttagagaaa tcgatgctca cgtatgcaac gtaatgcgcg
     2161 tttgattttt tgtcgaccct atagtggcgc gcaccggggg gaatcacctc ttcaatgtac
     2221 tgcaatactt ttgattttaa ttcggcgcca ttgtcgacgg tattttcttt tagatacgcg
     2281 agcatagcgt cagcgaaagg acgcacttta ctcggcgcct tgagaagcaa cgcagtcact
     2341 ttttcgcgaa aatggaaatt gggcaatttg ttaattttat accgttgatg gatgactggc
     2401 cgttgcaaat cttgaatttt gtgtaaaatt gcagatttat tggctgtttc ttttacgtcc
     2461 aaaagtggca gaagcgattt ggtgtatccg ccgaaagtcg ccggagcatc gcgtagaatt
     2521 ctttctatac tgtctttaaa ggcaatatct tctttaagaa ataaaatttt ttgccataca
     2581 tacgaaaatt caaagccgtt gatatgttct tcggttttgg gttcgctgcc gttgagtgat
     2641 ttcaacgcct ccagcttttt tgagactaaa tcgtacgcca ccgcgtaacg catcgattcg
     2701 ggttctgtgc cggtttcgcg aaaacggttc gtctgttcgg ctttaacccc atcgtacgca
     2761 agctctttgt cttggaatga aaaatagcta atcgcttgca caacagtcat aaaaaccacg
     2821 acgcacacaa gaataatttt tgccatgaac gtcgttttgt ctttcgtcga atttaaataa
     2881 ataatcacga tgacaaaaag acccaagatg gtgaataaat caaacgccgt ttgaaaagta
     2941 ccgcgatcca tccccccttc acgacttaaa gtatttagcg ccgctggcac caaagcgccg
     3001 caagtgaaac caaaaatcat accgatgacg ctccaccggt cgcgcccttt cgctaaaacg
     3061 gcgcgccaaa cgccaacggc aataagaagg acaaaggaag cgataatcgc aagactgatg
     3121 acatagcttg caacatcggc gtcgaaatcg tagtaatgcc cttcaaagtg atatacaaaa
     3181 ttggcatgaa tggtctgagt gacaaaccaa gcggcgaatc ctgccgttaa aataagtaac
     3241 gaataaaaaa gaattttacc gaatttagta ttgcgtggtt ctggatattc aaaaaagacg
     3301 cgcacaaaaa cagcaagtac ccacaaaacg caaagaactg tgttccaacg gtggaacgcg
     3361 gcgtatggat ggtacaacgt cgacgcgaca acgtaactca acacaaaaaa accgtagatg
     3421 agcatgccgt cggcgagcgc ttttgtcgcg cgcgatttat cttttatttg gtaaaaaata
     3481 gttgcggcaa taaaaagaaa aaccaccggt atcattgcac cgatggtata gtagtctaag
     3541 tacacgctac gtaagaaatc cataagttaa tgtgacaaaa gacattaggt ctgtcaagtg
     3601 taaataaaac gacgaattca aaaactgatt gaaacaatga gcacatacta cgttgaagat
     3661 accccatgga aataatccac atcattggcg caggcggcgt tggtatgagc gcgctcgctc
     3721 ttttagcaaa gcggcgtggt gcaacgatca ccgcttcgga caaaaataac tcatcgtatt
     3781 tacaaaaact cgcctcgcac ggtattcaca catgggtggg gtcgcagccc gaaaaaatac
     3841 cacaggacgc gcacgtttat tactcgagcg cggttaaagc agacgacccc gagcgagact
     3901 ttgctgaaac gcatggtttt ctatgcgagc cgcgccactc gctcttaaag cgcatcacgg
     3961 cggattactt cacgattgcg atcgccggct gtcatgggaa aaccactacc agcgcgtggc
     4021 tggcggactt actcatccgc gccgggaaag atcccaccgc tctcatcggc ggcacggtgc
     4081 cagagtggca gtcgaactac cgcgagggca gcggaaaact ccacggcaaa cccatccttg
     4141 tgatggaagc cgacgagtcc gatcgcagct ttttgtcgat cgatgtcgat atcgcgctca
     4201 tcaccaatgt ggatttagac cataccgaca tccatcaaag tctcgctgcg ctcgaaaacg
     4261 atttttgtgc gtttgcagac accgcgcggc aaaataacgg caacattttt ttaagcagcg
     4321 agtgtgatgg cgcgttactc gcacggctca ctcccgctga aaaagaggcg cgcgcacaag
     4381 taatcatagc tatggaaaaa catagcatcg ccgcgcaagg catggagtat ccagttggcc
     4441 ttgcgggtgt gcacaacctc aagaacgcga cattgatcgt gcaactcggt ttgacgttgg
     4501 gcatcccacc gacggttatt gctgcgtcgc tgcaagaatt ttcaggcgtt aaccgccgca
     4561 tgcagcgcat tgccacattc cctgaacgcc acctcacggt gatcgacgac tacgcacacc
     4621 acccacacga agtcgaagcg acactggccg cgcttgaacc gcaatacgat cgtcttttaa
     4681 ttttttggga gccacaccgt ctcagtcgct ttgtacattt ttaccgcgag tttgcggcgg
     4741 tgttagaaaa atacgcggca gagcatcaac tctttttact gccaatttac gcctcaggcc
     4801 acaaggcgga ggattatccc cactacgaaa aattattcca gcagtttacg aaatcccact
     4861 ttgtgtatat cgctcaacct agcgaatttt ctttggaacc attggcgttg gatggccgca
     4921 acacagcggc agtttttatg ggggcggggg caagcagcga atacgcgcag gcatttgttg
     4981 aattcgtaag acaacaattg acacaaaaaa ggtagcgagc ctcatttact ttttaatgtt
     5041 gggctgtgtt accacttcaa accgatttaa agaccgaact taaagaaggc ttccaccgcc
     5101 acttaaattt ttcgttgggg cgcactctcg acgaactcac caacgaagac gcgtatattt
     5161 ccctttcgtt gtcgttgcgc gatttgatga tgcggcggct gatgaaaaaa cgccagcgca
     5221 tgcgccaaaa aaagcgcgtc tactatctct cgatggaatt tctcatgggg cgcatgcttc
     5281 aagtggcgct cgtcaatatg gaaatgacca ctatcgccaa agaagttttg tctgagatga
     5341 aaattaattt ggacgaatta tatgccatcg aacccgatgc tgcgctcggc aacggcggct
     5401 tgggtcgtgt cgcggcgtgc gttttagata gcatggcgca ttacgatttt tcgtgcatcg
     5461 gcgcaggtct ctgctaccaa tttggtcttt ttgagcaaag ctttcacgat ggcgtacagt
     5521 acgaatcgcc cgacgtgtgg ctcaccggta attacccatg gctcatcgat cgccacgaca
     5581 aacgatacga catccatttt tatggtcatg tcgaacggca aggtgctcgg gcaacgtggc
     5641 gccatggcga cagcgtagtg gcgatggcgc gagacatgct catcccggga aacggcagcg
     5701 aaggtattgc aacgctacgc ctgtggtggg cagagcatgg cagcgatttt ggtttggatt
     5761 atttccagca cggcgattat ctaaaagcga tggagcggca aatttattcc gagtctatct
     5821 cgaaagtgct ctatcccaac gataataatt tgagtggcca ggagctccgc ctaaagcaag
     5881 aatatttttt aagtagcgcg accatccaag atgcgctttc gacgtttaag tttgaggggg
     5941 ggaacttttc gctgctggcc gatcgcgttt tcttccaact gaacgacaca caccctgcga
     6001 ttgcgattgc agaactcatg cgcattttag tcgaccgcga aggcatcgaa tggggaaaag
     6061 cgtggagcat cacccaaaag tgtatgggat acaccaacca caccattatg cccgaagcgc
     6121 tcgaaaaatg gaatctcgat ttattcggtc gtgtgttgcc gcgtcactta gaaattattt
     6181 acgacatcaa ctttcgtttc ttggacgagc tgcgtgcaaa aggttttgac caagacgcga
     6241 ttggccgtgc gtcgattatt gaggaaggtc atcccaaaag agtgcgcatg gcggcgcttt
     6301 cggttgtggc ctcctcggcg gtgaacggtg tttcggcgtt gcatagtaat ctcgtaaaga
     6361 cgcgcctttt ccccgattac gcaaagcttt ggccggagaa attctcgaat aaaaccaatg
     6421 gtattgcgtt tcggcgttgg ctcatcgcag caaaccccaa ccttgcaaac ctcatcgata
     6481 ataccatcgg cgacaagtgg cgcagtgaac atagcttgct tgctgcgctc gctacacaca
     6541 aggacgacgc cgcgctgcac aaccactgga aaaaaatccg cactgacaat aaaaagcttt
     6601 tagcagcggt gataaaagag cgcacgggga taactatccc caccgattct tttttcgata
     6661 ttcaagtcaa acgcatccac gaatacaaac gccagctttt gaatttaatg cgggtggcgc
     6721 gttgttgcct cgatatcttg gcgggcacgt acactatcga aacccaccgc acgtttattt
     6781 ttgcaggcaa agctgcgccg ggataccacc gcgccaaact catgatacgc ctcgcgaacg
     6841 ctctttccgc gtatatcgaa aaaaatccgc tcatcaaaaa gcgcattcaa ctcgtgtttt
     6901 tgcctaacta tgcggtgtcg ctcgccgaga ggatttttcc tgcggcagat ttatccgagc
     6961 agattagcac tgccggcacc gaggcgtcgg gtacaggcaa tatgaaattt atgctcaacg
     7021 gcgttgtcac cttgggcacg ctggacggcg cgaacgttga gatccgcgaa gaagtcggcg
     7081 acgccaacat ttatattttt ggccacaccg tcgaaactct caacgcattg caggagactg
     7141 gttacaatcc tgtcgagttt gcaaaaaacc cgcgccttgc tgaaattttt gaaatgtttg
     7201 aaattctgca cggcgacttt cgcgagtttt tcgacgcact cacctaccaa ggcgatccgt
     7261 atttccatct tgccgatttt aaaaattatt gggaggtgca ggaaacgatt atgacggatt
     7321 acttgaatag cgcttcttgg ctctcaaaag aaattatgaa caccgcacac tcgggaaaat
     7381 tttccgccga ccgtaccatc tacgagtacg cacgcgatat ttggaaaatt gtggagtgag
     7441 aatgcttttc cccgtcacag gttttcccgt tcctaaaaac tttcagaaaa gcatccccac
     7501 ccacgcggga cgcatccacc taacaagctt tcaggatgca gaacagagtg cgtggcgtat
     7561 atctaaaaag acagatgcac tttttttaac gaaagcggca catttaagta agctcttata
     7621 ccgcatttta aagaaagtct ctatacaacc ttccatgagt tctgaacaat acttgtccgc
     7681 tgcgcacagc tttgctatcc ccgaagcaag cgtgggcttt ccgttttgtg ggctttattt
     7741 tttgtcgata cacaatccca ctttgctgcc cctgcaccct acactggtgg tggaaaaaag
     7801 cgctgcggtc ataggcgctt tggacggcga taccacgctt gaacgtcttc ttaaactctt
     7861 gaacgacaac cccacctcaa tcgacgcaca aatccaagca ctccttaaag cgcatgccga
     7921 tttttttacc gacgaggaga aatcacattt tttgcagatt gttcactaca cagcaggcgc
     7981 caaaacacaa gcgaacgcag cgccattata tggttacgac gcgccggtgg gcgctcccca
     8041 actacgcagc cacgacaaag cttggatgca gtcactcgtg ctcattgcaa aaattgtccc
     8101 tgtatggctc gtggacatac gccgccgcta tggcatcgac gcgcgccgtt tggacgacgt
     8161 gcctgatgcg gaacttgtgc atctcaaaag ttttggcgtg aatggaatct ggctcgtggg
     8221 tttgtggcaa cgcagtgcag cgtcggcgcg tatcaaaaat ctttcgggtg gaaaaaatta
     8281 tgcatccgca tattccatct ttgactaccg cgtcgccgac gaatggggtg gagaagatgc
     8341 gctcgaacaa ttctatcacc gctgcgcccg tcatggtatc cgcttggggt gcgacatggt
     8401 tcccaaccac accggcatcg attctaagtg gctgcgcgaa aatccagatt ggtttatgca
     8461 agtagaagaa tctccttttc caaattatcg ctttagcggc gaaaatcttt cggcagatgc
     8521 tccgatggaa atccgcatcg aagacgggta ctatacccac cgtgacgcgg cggtggtgtt
     8581 tgaatataaa aaaaataacc gcacgcgcta tatttaccac ggcaacgacg gcactggcct
     8641 accgtggaat gatactgcgc agcttgacca cacccgtgcc gatgtgcggc aagtcatcat
     8701 cgacaccgca ctttcgctca cacgctattt ttccgtcatc cgctttgacg ctgcgatgac
     8761 actcacgcgc gagcactacc accgcctgtg gttccctccc ccaagcgctc cgcctgcaat
     8821 ccccagccgt tcggcggcga cactttccgc gcaagacttt tctgcacaaa tgccgcgtga
     8881 gttttgggca gatttggtcg aagcgttccg caaaaaaagt cccgagacgc ttctcatcgc
     8941 cgaggcgttt tggatgacag aaatgttttt tgtgcgcaac ctcggcatgc accgcgttta
     9001 taatagcgcg ttcatgcacg cactgcgcga cgaaaaaaat cacgagttcc aaaaaacgct
     9061 caccactttc cgtgagcacg atgagggaac gctcacacgc tttgtaaatt atttgagtac
     9121 ccccgacgaa gaagccgcgc gggcacatt
//
LOCUS       DAOMPD010000035         9056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3299_length_9056_cov_2.790801, whole genome shotgun sequence.
ACCESSION   DAOMPD010000035 DAOMPD010000000
VERSION     DAOMPD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3299_length_9056_cov_2.790801"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1143
                     /locus_tag="PLY93_02165"
     CDS             148..1143
                     /locus_tag="PLY93_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68315.1"
                     /translation="MDTLYIENAAKKYALTATIESRIAPQKTVFIDDYEEHFSKKKPY
                     LNKLFERNIFIALKKNNFIHEAINRQSVNPTEPHYDFTTAYNCSYGCEYCYLQSYFNS
                     PDLVFFVNHDDILNAMREKLVAQGKKRVWFHSGELSDALALSHLTGALPLYWDFFRFN
                     PTAFLELRTKSINVSVLRTLPPLPNVVVSYSLSPHEQARKLDHGAPTVLQRLTAMEQL
                     KQRGFRLGAHFDALMQHDRLGERVEHLVKIIAQRIGFDAFEFFSVGTLELDAPLYSEA
                     KQNYPNSLMWQSGLDENAATKIAYAAVVRERIHSEIQQILKKHGCPEEKIFFCKE"
     gene            1140..1781
                     /locus_tag="PLY93_02170"
     CDS             1140..1781
                     /locus_tag="PLY93_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRP68316.1"
                     /translation="MNPRYIIGITGASGVDYALRFLWRLSFVAGKSDVIVSPNFYQVL
                     SAETNRGQQPRTENVGIQAHDVFKGIKILIEEMYGKMANRHHFQAADYHNIGAEAASG
                     SVRYNAMVVLPCSMKTVASIAHGLSQNLIERAADVSLKERRPLILCPRETPLSPIHLR
                     NMLTLTEAGATVMPLMPGYYHKPSTFTDLYDFMCDRIFQHIGIDERVIQPWLS"
     gene            1769..2467
                     /locus_tag="PLY93_02175"
     CDS             1769..2467
                     /locus_tag="PLY93_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68317.1"
                     /translation="MAFVKLLFVFSLFFSLNAIPVEYELDKPLDFNKTGRFVHTPNLY
                     VQHQCSATQVKKLVQEIAEFSTKKSPLSVLDAPLQPLQVRLFKNAKVYRDEFHFSKHR
                     HAHYNERLEIVTSYCDAPFSEIEEQWILHRLAETRLRTWQKYFLAETIAHPTRAFRET
                     GQAKPVSFLYLFLSNHKPDNAERKTMHALFAYLMKKKVWDSFCEALLLQQNFDDTGIE
                     IFEKLTGEPITNGK"
     gene            complement(2474..3487)
                     /gene="rfaE2"
                     /locus_tag="PLY93_02180"
     CDS             complement(2474..3487)
                     /gene="rfaE2"
                     /locus_tag="PLY93_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRP68318.1"
                     /translation="MLNPFLYTVILDRDRTINEDPGYLNDPDKVVLMPHAAEGIHLLN
                     DFGLRIFVASNQSGIARGLITNAQLAAVNMRISALLAKEGAHLENFYICPHTDDDNCV
                     CRKPKPGLIQKIWKENTFDAMRTFLVGDRARDLLCGQALGMRGILVGDDELDEPENCV
                     FRAAHLGEAAAFILETIFEEATRQKIFTDAKDFLAYFENGRKNYHKIVFTNGCFDLLH
                     SGHVQLLSQARALGDYLILGLNSDRSVRVLKGDTRPLNKAIDRARVLAQLYSVDAVVI
                     FDEDTPIELLQKIRPDIQVKGGDYIKEDLPEYPIMQKMGKKIVILPFRKGYSTTSLIQ
                     KMQ"
     gene            complement(3487..3699)
                     /locus_tag="PLY93_02185"
     CDS             complement(3487..3699)
                     /locus_tag="PLY93_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68319.1"
                     /translation="MPLDSSRKKIEKESPMNEQQQFYECPECASIVERVYQNGVCRAC
                     LNKKLQAVRLVIDTNHILKAGKLAAY"
     gene            complement(3806..4930)
                     /locus_tag="PLY93_02190"
     CDS             complement(3806..4930)
                     /locus_tag="PLY93_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRP68320.1"
                     /translation="MESVATHTASRAKNQPIQYLRPTLSRNELKSVLESLVLEEVSFG
                     QGVLNFEKAFARTFDFNHAAAVDSYTSAFHLALIACDLAKDDEIILPVSSPLAMLDAI
                     HYTGARPVVIDLAKDSFHADVDTYLNAITERTKIIFLPYTFGSYKDYSTLYEKLSVAG
                     LRKPKERYIRLIEDISHTIGHEFHGRQAGENADIAIAGLNEDHLMTIGKGGVVLTDSH
                     NHFAVIKDLRVQGGNRPYRMRFDYAITDYQAAMGMEQLGNLSAMIERRRRIGGLYNEA
                     MEKSRLKTYFTSPDADVCGSFPIVSETDLAHTQSYFASLHIETRRIFPNGPLHQIMGL
                     NVLNFPNAERLYQRGVMLPLYPMLNKPSVEKITAAIRGFY"
     gene            complement(5129..7027)
                     /locus_tag="PLY93_02195"
     CDS             complement(5129..7027)
                     /locus_tag="PLY93_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRP68321.1"
                     /translation="MRIYSEHPIQSDLRLLYANALVDKAKALHLADTRAWRTLIHYDS
                     FLGITRSEARDKEKKGDFFLSPQGERNPESELKATILALFLDTQSSDPARCRFPAREI
                     YLTRALAIDTSRLGPVNCREYDGWRKNNAAKGASIVFASFFMGNPSSLFGHTFLRLHN
                     AHGNALLDASFNFAANAPDDENAIVYAWRGMTGGFPGLFSMHPYYVKINEYNDMESRD
                     LWEFTLDLTENELHFLEAHLWELSYASFPYYYLDENCSYQLLALLEAVRPQAHLNSRF
                     SYFVAPTDTLHTLFENNFFTGESKYRASSSTRYFTFYENATPVARSTMVLLKKNRALP
                     QTHTNNDTQAVDMLIEYYKYTNEYNMAAWPVENREHYQNLLSWRAQVPHENTLEKFVR
                     AHNEENPLTGFKSSSIALGYAYEKTLGGAVALDYRPALRTLADSWRGFSPWSQIQMLQ
                     VALSYFPHTQSLRLDEFNIVDIFALSPWQAHIHKISYRLRTGLYRHNALQSYNAATLS
                     WDSTAGIGITLLPLKRVGFFVLAEANAYVRPAWQSAVQLAPSLLTGFKFNWSPAFATT
                     LSYHIDYAFLSPQIIEQKTVIHTTLAVAQNTAIEVKFFYAWAAVETLPSATYLDAIRG
                     DFSLRLYF"
     gene            complement(7077..7571)
                     /locus_tag="PLY93_02200"
     CDS             complement(7077..7571)
                     /locus_tag="PLY93_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRP68322.1"
                     /translation="MKKISLSLAAVAAVAFAVPTAISAAYGAAGCGLGAIVFGSKPGI
                     VQVLAATTNGTFASQTFGITTGTSECGGGLVKVEAEQKVYAYNNFNQLQKEIAQGKGE
                     RLQTMAYLMGCNAESLNGFTTVAQKSYSTIFADKSTNSDMMLERLRTSVKADATLASQ
                     CSAL"
     gene            8071..>9056
                     /locus_tag="PLY93_02205"
     CDS             8071..>9056
                     /locus_tag="PLY93_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68323.1"
                     /translation="MKDGALLHVEYIAETDAKATVDIRAAILPDALCTGDALPKYLPK
                     NAFRSPNKLESGQCSVTGKPFIVRSVTLEPAQILKRHDVTLLTLSKSGEKKQLPFLIE
                     KNGYAWGYNFSNVEPSPVTPAPGLGLQAFPLDSTMETIQTVDVNGKREILLPSQNIFR
                     KWLRLDPRVGLYYTPLLPTANINLLNFGPLGFTLENSIAIPEMSFLPLKKYSLRLRGG
                     INVGYHTFAQELYQNNLAVADGHIMLLPVLLQATLYWDLKPKNMKLTVSPYLRLADGI
                     VFSTVSTQLKPQYVSLLNPANANDVSRSGSYIGNGFGASLGVELKPESWSVA"
BASE COUNT         2395 a         2043 c         2189 g         2429 t
ORIGIN      
        1 aaaccggccg cgagactttt tcgtatgcat tccacaataa cgcccctgtt tcgctttacg
       61 cgattagccg cgaagacgcg ctccagtttt ttgagcacaa tcccggcgtt aaaatgaagt
      121 tacaatacga attctaattt catgcgggtg gatacattat acattgaaaa cgcggcgaaa
      181 aaatatgcgc tcaccgcaac gattgagtcg cgcatcgctc ctcagaaaac agtttttatc
      241 gacgattacg aagagcactt ctctaaaaaa aaaccgtact taaacaaact ttttgagcgc
      301 aatatattta tcgctcttaa aaaaaataat tttatacacg aagcgataaa ccggcaaagt
      361 gtaaatccca ccgagccgca ttacgatttc accacggcgt ataattgtag ctacggctgc
      421 gagtactgtt atttacaaag ttattttaac tcacccgatt tagttttttt cgtcaaccac
      481 gacgatattc tcaacgccat gcgcgaaaag cttgtggcgc aaggaaaaaa acgcgtgtgg
      541 tttcacagcg gtgagttgag cgatgcgctg gcgctttcgc atttgacggg cgcgcttccc
      601 ctctattggg atttttttcg tttcaacccg acggcattcc tcgagttgcg cacgaagtcg
      661 attaacgtga gcgttttgcg tacgttgccg ccgctgccga atgtggttgt aagttacagc
      721 ctttcgccgc acgagcaggc gcgcaaactc gaccacggtg caccgacggt gctgcagcgc
      781 ctgactgcaa tggaacaact aaagcaacgc ggttttcgtt taggcgcaca cttcgacgcg
      841 ttgatgcagc acgacagatt gggcgagcgc gtggagcatc tggtcaagat tatcgcgcaa
      901 cgcattggct ttgacgcctt tgaatttttt tcagtgggca ctcttgagtt ggacgctccg
      961 ctttattctg aagcaaaaca aaattatcct aactcgttga tgtggcaaag cggcttggat
     1021 gaaaatgcgg caacaaaaat agcatacgcc gctgtggtgc gcgaacgcat acatagtgaa
     1081 atccaacaga tcttgaaaaa gcatggttgc cctgaggaaa aaattttttt ctgcaaagaa
     1141 tgaacccacg ttacatcatt ggcatcaccg gcgcatcggg ggttgactat gcgttgcgtt
     1201 ttttatggcg gctttcattt gtcgcaggca aaagcgatgt gattgtctct cccaattttt
     1261 atcaggtgtt gagcgcggaa acaaatcgcg ggcaacaacc gcgcacagaa aatgttggca
     1321 tccaagcgca cgatgtattc aaaggcatca aaatactcat tgaagaaatg tacggtaaaa
     1381 tggccaatcg gcatcacttc caagctgccg attaccacaa cattggagca gaggcggcga
     1441 gcggctcggt gcgatataac gcgatggtgg ttttgccgtg tagcatgaag acggtggcgt
     1501 cgattgcgca tggccttagc caaaatttaa tcgagcgcgc cgccgatgtt tcactcaagg
     1561 aacgccgccc gctcatcctt tgcccgcgcg agacgccgct gagcccaatc catttgcgca
     1621 acatgctcac cctcaccgag gcgggcgcaa cggtgatgcc tctcatgccg ggctactacc
     1681 acaagccgag cacattcacc gatttatacg attttatgtg cgatcgtatt tttcaacaca
     1741 tcggtatcga cgagcgagtg attcagccgt ggctttcgta aaattactat tcgtgttctc
     1801 tctatttttt tcattaaatg caatccccgt tgaatacgaa ctcgataaac cgttagactt
     1861 caacaagact ggccgtttcg tccatacacc caacttatac gtgcaacacc aatgcagcgc
     1921 tactcaagta aaaaaactcg ttcaagaaat agcagagttc tcgacaaaaa aatcgccgct
     1981 gagtgttctc gatgcaccgt tgcagccgtt gcaggtacgc ctattcaaaa atgcaaaagt
     2041 gtaccgcgac gaatttcatt tttcaaaaca ccgccacgcg cactataacg aacggttgga
     2101 gatcgtcaca agttattgcg atgcaccgtt ttctgaaatt gaagagcagt ggatactgca
     2161 tcgtctcgcg gaaacgcgat tgcgtacgtg gcaaaaatat tttttagcag aaacgattgc
     2221 tcaccccacg cgtgcattcc gagaaaccgg ccaagccaag ccagtatcgt tcctgtatct
     2281 tttcctgtcg aaccacaaac ccgataatgc tgaacgtaaa accatgcatg cgctttttgc
     2341 ttatctcatg aaaaaaaagg tgtgggactc tttttgcgag gctcttcttt tacagcaaaa
     2401 ttttgacgac accggcattg aaatttttga aaaactgact ggagagccaa ttactaatgg
     2461 caaataaaat aatttattgc attttttgaa taagactcgt cgttgaatag cctttacgga
     2521 aaggtaaaat gacgattttc ttgcccatct tttgcatgat agggtattcg ggtaaatctt
     2581 ctttgatgta atcgccgcct ttgacctgga tgtcggggcg gattttttgt aggagttcga
     2641 tgggagtgtc ttcgtcgaaa ataacaaccg cgtcaacgga gtagagttgt gcgagaacgc
     2701 gcgcgcgatc gattgccttg ttgagcgggc gcgtgtcgcc ttttaaaact cgcacactgc
     2761 ggtcggaatt taagccgagg attaaatagt ccccgagcgc gcgcgcttgc gacaaaagtt
     2821 gtacgtggcc ggaatgcaac aaatcaaaac aaccgttggt aaaaacaatt ttgtgatagt
     2881 tcttacgtcc attttcaaaa tacgcaagaa agtctttggc atccgtaaag attttttgtc
     2941 gcgttgcttc ttcaaaaatt gtttctaaaa taaacgccgc tgcttctccc aaatgcgcag
     3001 cgcgaaaaac acaattctct ggctcgtcga gttcgtcgtc gcctaccaaa ataccgcgca
     3061 tacccaaagc ctgaccgcat aacaaatcac gcgcgcggtc gcccacgagg aaagtccgca
     3121 ttgcatcgaa agtattttct ttccaaattt tttggattaa cccgggtttg ggtttgcggc
     3181 aaacgcagtt gtcgtcatcg gtgtgcgggc aaatataaaa attttcaagg tgcgcacctt
     3241 cttttgctaa aagcgcggag atgcgcatgt ttacagcagc taattgcgca ttcgtgatca
     3301 atccccgcgc aatacccgat tggttactcg cgacaaaaat acggagacca aaatcgttta
     3361 gcaaatggat accttcagcc gcgtgcggca tcaacacaac tttatcggga tcgttcaaat
     3421 agccgggatc ttcgttgatg gttctgtcgc ggtcaagaat tacggtgtat aaaaaaggat
     3481 tgagcatcaa tatgcagcaa gctttcccgc ttttaagatg tggttggtat caatcacgag
     3541 gcgcaccgct tgcaattttt tattgaggca tgcacgacag acgccgtttt ggtacacgcg
     3601 ctcgacgatg gatgcacact cagggcactc gtaaaattgt tgctgctcat tcataggcga
     3661 ttctttttcg attttctttc gagatgaatc gagaggcatc gaagtaaaga tgtttctgta
     3721 tgtgtaactc aacgtaaaga tgatttcaaa aaaaaatcaa ttttttttag ttattatgtc
     3781 gtacgacaaa cgacataata aaactctaat aaaagccacg aatcgctgcg gtaatctttt
     3841 ccactgaagg cttattgagc attggataaa gtggtaacat aacgccgcgt tggtataaac
     3901 gctcggcgtt agggaaattc aacacattga gccccattat ttggtgtaac gggccatttg
     3961 gaaaaatgcg gcgtgtttca atatgcagcg atgcaaaata actttgagtg tgcgctaaat
     4021 cggtttcaga gacgatagga aaagagccac atacatcggc gtcgggagag gtgaaataag
     4081 ttttcaatcg gctcttttcc atcgcttcgt tatagagacc gccgatgcgg cggcggcgct
     4141 caatcatcgc cgacaagttg ccgagttgtt ccattcccat cgccgcttga taatcggtga
     4201 tggcataatc gaaccgcata cgataaggac gattaccgcc ttgtacacgc aaatctttga
     4261 tgacggcaaa atggttgtgc gagtcggtga gcaccacgcc tcctttaccg attgtcatca
     4321 aatggtcttc gttgagaccg gcaatcgcga tgtcggcatt ttctcctgct tggcgtccat
     4381 ggaactcatg tccgatggtg tgcgaaatat cttcaatgag gcggatatac ctctctttag
     4441 gtttacgtaa acctgctacg ctgagttttt cgtaaagcgt actataatct ttgtaagagc
     4501 cgaaagtata aggcaggaaa ataatttttg ttctttctgt gatggcattc aaatatgtat
     4561 cgacatccgc atgaaaagaa tctttcgcta aatcgataac aacgggacgc gctccggtgt
     4621 aatggatggc gtcgagcatc gccaacggcg acgatacagg taaaataatt tcatcatctt
     4681 tggctaaatc gcacgcgatg agcgccaagt ggaatgcaga tgtgtacgaa tcgacggcgg
     4741 cggcgtggtt gaaatcaaac gtgcgcgcga atgctttttc aaagttgagt accccttgac
     4801 caaacgaaac ttcttcgagc accagcgatt cgagcaccga cttgagttcg ttacgcgaaa
     4861 gcgtgggccg cagatattgt atcggctggt tttttgcgcg tgaagcggta tgcgtcgcta
     4921 cagattccat agtattatat ctcatccaaa tgatgcgaca taatacgaca agctattttc
     4981 gtagttttgg atacgagctt gtgcgagtta ccgtacaggc tcatgcgtat ccacaaactc
     5041 acgctccgtt tggataggat cgcaatctct ctttaatttt ttaatgaatt atagtcgggc
     5101 aaagagtaca cagtggaatg cacgccctct aaaaatacaa tctaagagaa aaatctccgc
     5161 gtatggcgtc aaggtaagtt gcagatggca gtgtttcaac cgccgcccac gcgtaaaaaa
     5221 atttaacttc gatcgcagtg ttctgtgcga cggcaagcgt ggtgtggatt acagttttct
     5281 gctctataat ttgtggcgac aaaaacgcgt aatcgatgtg gtatgaaagc gttgtagcga
     5341 acgcgggcga ccagttgaat ttgaatcccg ttaaaagcga aggcgcaagc tgtactgcgc
     5401 tttgccatgc ggggcggacg tacgcgttgg cctcggcgag tacaaagaag cccacgcgtt
     5461 tcagcggcaa aagtgtgatg ccgatacctg ccgtcgaatc ccacgagagt gtggcggcgt
     5521 tgtagctttg gagcgcattg tggcgataaa ggccggtgcg tagtcggtac gatattttgt
     5581 ggatgtgcgc ttgccacggc gatagcgcaa aaatatcgac gatgttaaat tcatctaaac
     5641 gtaaggattg tgtgtgggga aaatacgaga gcgcaacttg cagcatttgt atttgcgacc
     5701 acggtgaaaa tccgcgccaa gagtcggcga gcgtgcgcaa cgctgggcgg taatccaacg
     5761 cgacagcgcc gccgagtgtt ttttcgtatg cgtacccgag agcgatgctc gacgacttaa
     5821 aacccgtgag cggattttct tcgttgtgag cgcgtacaaa tttttccagt gtgttttcgt
     5881 gcggcacttg cgcgcgccaa gagaggaggt tttggtaatg ctcccgattt tctacaggcc
     5941 acgcggccat gttgtattcg ttggtgtatt tatagtactc gattagcata tccaccgctt
     6001 gggtatcgtt gttcgtgtgc gtttgtggca gagcgcgatt tttttttaag agaaccatcg
     6061 tactacgcgc aacaggcgtg gcgttttcat aaaacgtaaa ataccgcgtg gagctcgacg
     6121 cacggtattt actttcaccc gtaaaaaaat tattttcgaa aagcgtgtgc agcgtatcgg
     6181 tgggtgcgac aaagtaagag aagcgactat tgagatgcgc ctgtgggcgt actgcttcta
     6241 agagcgctaa aagttggtac gaacaatttt catcgagata gtagtaagga aagcttgcgt
     6301 aactcaactc ccacaagtgc gcttctaaaa aatgcagttc attctcagtg aggtcgagcg
     6361 tgaactccca taagtcgcgg ctttccatat cgttgtattc gttaattttt acgtaatagg
     6421 ggtgcatcga aaagaggcca ggaaatcctc ccgtcatgcc gcgccacgcg tacacaatcg
     6481 cgttctcgtc gtcgggagca ttggcggcaa aattaaaaga cgcgtcgagg agcgcgttac
     6541 cgtgtgcgtt gtgcaagcgc aaaaacgtgt ggccaaagag cgaagaggga tttcccataa
     6601 aaaagctggc gaacacaatc gacgcgcctt tagccgcgtt atttttccgc catccgtcgt
     6661 attcgcgaca gttcactggt ccaagacggc tggtgtcgat agcgagcgcg cgcgttaaat
     6721 aaatttcccg tgcggggaag cggcagcgtg cgggatcgga gctttgtgta tctaaaaaaa
     6781 gagcaagtat cgttgcttta agttccgact cgggattgcg ctcgccttgt ggcgaaagaa
     6841 agaagtctcc ttttttttcc ttgtcgcgcg cttcgcttct tgtaatcccc aaaaaactat
     6901 cgtaatggat gagggtgcgc catgcgcggg tgtcggcaag atggagcgct tttgctttat
     6961 cgacgagtgc gtttgcgtag agaaggcgga ggtcgctttg aatggggtgt tcggagtaaa
     7021 ttctcatcgt acacagagag catacgatga gataaagaat tttattcagt ggccaattaa
     7081 agagcagagc actgagaagc gagggtggcg tcggctttta ccgatgtacg cagacgctca
     7141 agcatcatgt ctgaattggt cgatttatct gcaaaaatag ttgagtagct tttttgcgca
     7201 actgtggtaa aaccattcaa agattcagcg ttgcagccca tgaggtatgc catcgtttgc
     7261 agtctttcgc ctttaccttg agcgatttct ttttgcagtt ggttaaagtt gttgtaagcg
     7321 tacacttttt gttctgcttc aacttttact aatccgccac cgcattcgga tgtaccggtg
     7381 gtgataccaa aagtttggct ggcaaacgtg ccgtttgttg tagcggctaa aacctgaacg
     7441 atgcctggtt ttgaaccaaa aacgatggca cccaagccac aaccggctgc gccgtatgct
     7501 gctgaaattg ctgttggtac agcaaatgct accgctgcaa cagcggctaa agagagagaa
     7561 attttcttca tgattaaatc ctccaataag tcgggaaaat cccgctaaga cagtgaaaat
     7621 tatttagagt cgctgccaag ataatttagg tgtggagatt acagtaaaca tccaagatcc
     7681 tgtgcgccaa ctcgctggta caattttttc caagttttgt acacgctgcg cgagttgggg
     7741 catgtaagct ctcagggaca attatcctaa ttttttagat tatgtcgcat gaagggataa
     7801 ctattccagt ttttcgaatt atgtcgcatt aatggataac tatgcactgc gggctgtttg
     7861 tttgcagtat tatgaatact cacaagcacg atagttctcg caaacgtaat ttacggataa
     7921 ttttcaaaaa aatgtcgtaa tttttgattt tgttgcgtcg aaaatggtgt aatgtacatt
     7981 cacaattttc ttcgcgttct actgtttttt ctttttttag ggataactat cctttttgcg
     8041 acagcgccca aagtcgacgt agttctcgaa atgaaagacg gtgcactttt gcacgtggaa
     8101 tatattgcag aaaccgacgc caaagccaca gtggatatcc gcgctgcgat attgcccgat
     8161 gcgctatgta cgggagatgc tctaccaaaa tatttaccta aaaacgcgtt taggtcgccc
     8221 aataaacttg aaagcggaca gtgcagcgtg acaggtaaac cgtttatcgt acgttcggtg
     8281 acgcttgaac cggcacaaat tttaaaacgg catgatgtta cgcttttaac gctttctaaa
     8341 tcgggagaga aaaaacaact tcccttctta atagaaaaga atggttatgc gtgggggtat
     8401 aatttttcta acgtcgaacc atcgccggta acacccgcac ccgggttggg actacaagcg
     8461 tttccgttgg attcaacgat ggagactatt caaaccgttg atgtaaacgg caagcgcgaa
     8521 atacttttac ccagtcaaaa tatttttaga aaatggctac gtctcgatcc gcgcgtgggg
     8581 ctttattaca ctccgctttt gccaacggct aatatcaacc tgttgaattt tggtccgttg
     8641 ggattcacct tggaaaactc gattgccatt cctgagatga gttttttacc tcttaaaaaa
     8701 tatagcctcc gcttgcgtgg cggtatcaat gttggctacc atactttcgc acaagaactc
     8761 tatcaaaata atttggccgt tgccgacgga cacattatgc ttctgcctgt gttgttgcaa
     8821 gccactctct attgggatct aaaacccaaa aatatgaaac taacagtgtc gccttattta
     8881 cgtttagcag atggtattgt ttttagcacg gtcagtactc agttaaaacc gcagtacgta
     8941 tcactcctca acccagccaa cgccaacgat gtatcgcgtt cggggagtta catcggcaat
     9001 ggatttggcg cttcgttggg tgttgagcta aagcccgaaa gttggtcggt ggcgtt
//
LOCUS       DAOMPD010000036         8995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3326_length_8995_cov_2.687584, whole genome shotgun sequence.
ACCESSION   DAOMPD010000036 DAOMPD010000000
VERSION     DAOMPD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3326_length_8995_cov_2.687584"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..900
                     /locus_tag="PLY93_02210"
     CDS             103..900
                     /locus_tag="PLY93_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68324.1"
                     /translation="MRVQNIPLLWILFLASAIPLFANSPDRAFCDKILGENKRFIDFI
                     NVPISNFADLEELADFRNAALEHYTAHKEFLSGHYPKTHAHIRNSQRILKDLYYKVLG
                     KYYQKDAREMLERNSAMIIETRDVRAKHLMKLGYRDLKVADIYKQKGYNHNIHLYSTK
                     IWFYIDAIKYARQAKRYALLAIIESQTPYADKDDFKQQTIDDHFNKPEPDEKRDFDRV
                     QTKLINLIHRKLVNDNYNFFFHLFDNYGYINSEKRNQYDDFISQTQK"
     gene            945..1409
                     /locus_tag="PLY93_02215"
     CDS             945..1409
                     /locus_tag="PLY93_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRP68325.1"
                     /translation="MLYSSASKYAVRALSYMTECIDQTVFTVDELAKGANVPKPYLSK
                     ILKQLVSGKILKSSKGPGGGYQFARSVDKISLYDIKVTIDGIQEFVDCVLGLDACNDA
                     APCPAHFMWKELRAQTTVALQTTTLPVAQRIMHEKALHPEGAKKRTQKKRLQ"
     gene            complement(1401..2477)
                     /locus_tag="PLY93_02220"
     CDS             complement(1401..2477)
                     /locus_tag="PLY93_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HRP68326.1"
                     /translation="MLNATRILTEGDVFYKKALESIEQAKMTVAFMVYIWQDDEIGRR
                     FEKALAAAAQRGVYVRLVVDAVGSLELPRSVIARLREAGVHVFIHHRLKIFRWQWLRF
                     LIRRNHRKIIIVDETIAFTGGFNIMRECSRKFFGKARWLDIALVVKERALINPIMEQF
                     EDACRRARHEKWAARLLTRTGNRVIFSHRGRVLTYLLSRYMKHRLLRAQKRIVIAVPY
                     FVPYGFWRRVLKQKLRKGVRVEIILSERSDLPWIDAVSFSLAKRLQKKGARVFLYRGN
                     ESLPRFSHTKLLMIDEWVLTGSANFDYRSMVLNLETMLAFRHPAGRFNPVLNELYSGS
                     RLAVKDELKGGLRAFLLKPFHWLL"
     gene            2732..3055
                     /locus_tag="PLY93_02225"
     CDS             2732..3055
                     /locus_tag="PLY93_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP68327.1"
                     /translation="MAERNETTTISDDMIFKGSIETDHAMLIQGRVSGNVIARARIQI
                     DEKADVQANVSARDLEILGSLQGNVLNAESVTLQPSARLTGDIQTAQLQIQRGAKHNG
                     NTSMR"
     gene            3108..5180
                     /locus_tag="PLY93_02230"
     CDS             3108..5180
                     /locus_tag="PLY93_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRP68328.1"
                     /translation="MDLFTKWYGFLYEQKNSFAETIYHRFLERLDTYTEGRLPDEIKR
                     EHVLRYLLPSVHLLKSPFVFPDMARAVSLIREHAPEKSSDSGFLQNSAKKIVIYGDRD
                     ADGVTSAALLTLFCREQLGITAQNLTTLVPREDDKYGIVGGVVQRIIDMKPDLLITLD
                     CGSSNRNELQQIRQATGCKTIIIDHHFIPPDPNDFPDVEAFINPKRLAESEFARDYCT
                     AAIVLRFVQAILFSYTRNFDQEIYLHTAQGEIYLKNGVQIPKKDLHQIQKHYSLRVKS
                     PISNECHDLEKIFAHTARENFSMQRLFHFFQKAPDAFSPVEIFNAVESASYRKVHAIL
                     APYIPLASIGLVADLMPIYADNRILVREGLDVITRQLDTLPIGLVALIKKLNLASSIQ
                     EQDLGFSLCPVINAAGRMGNAALALDALVEKDPLNAAKAAHALIQTNDARKEASQAAL
                     TLLDDALDSRSTHSPIAVAVHAHFHRGISGLIASKIAEKLGKPSVVMVPDGDTYRGSV
                     RAYKNENVYSLLESLSAHLIQFGGHKQAAGFSVAEENVPAFIDAVFAKASALYGKAES
                     NGDGMMIDPFRSFDPPLEIDISRLRTALWNELLTHAPYGVINPHPIVSILEAGKVEIS
                     PLGKTGAHAKIKFAPPADASIEGVWFFHGGAAESIATRENLIVSGEPQVGKFMGRVQY
                     RLKITKIS"
     gene            5524..>6230
                     /locus_tag="PLY93_02235"
     CDS             5524..>6230
                     /locus_tag="PLY93_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68329.1"
                     /translation="MNLNRYVPLLLLLGFFACREKPPIRSSDYMASLPEGEVKRKLIM
                     GCTSCHQMGVPVALRNTREEWLGTINKMLKLDEDLKLHLIPLSKEDLVDWLTKHGSGP
                     LEKRNIKIYSDVPTAKADYEEFSLGNVKGFYHDVAISGGRVFVADYFGNKLYSIDLKT
                     RKTESYDIPVSSKPGKPGGAHAIDTTRDGKLWVTFTKSEQVMQFDTKTNKFRIYNGFL
                     RDGNVQYFVLDADRNIY"
     assembly_gap    6231..6259
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6260..6873
                     /locus_tag="PLY93_02240"
     CDS             <6260..6873
                     /locus_tag="PLY93_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68330.1"
                     /translation="SKEILSRVNRDSGETKIFQTTRTQNMDEAAVHLYAAVADSKGRL
                     WYTETHGNHMGFLDPATGKTWEKPMALAWAGPKRLAIDKNDMLWIPELATGKITLYDT
                     VKESIAGQIELPIKGDYIYAVRRNPYNGDMWANGSGSDSLYRINPVTKAVRVYRLPRL
                     GTFTRTVAFDQEGNVYTSYASLPNKHTQMPHESAVILKVTPKD"
     gene            complement(7001..7879)
                     /locus_tag="PLY93_02245"
     CDS             complement(7001..7879)
                     /locus_tag="PLY93_02245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP68331.1"
                     /translation="MRKIVFFLLGSLTVHTVWGQQFRNIQQIKASGANAELLLYQSAD
                     DAIVEFRAINRETKNFIVEVNMQLDNMKSEKALPVKMKLPASTTRELKLFQISRIDAN
                     KAFYFRNLSWYLHADFGLSDQSNKPVKHNGIYNLMWVKGKTFRIDNGFNSYGAHRGDW
                     AYALDFKMPEGTEICAAREGVVTETESKFSVGGNDPALGDKANYIYIKHEDGSIGRYL
                     HFRKGGVRVKPGDKIKLGQKIGSSGNTGWSTDPHLHFDVIVPKPNGGYKTVPFKLRTK
                     KKASIEPVQGMSLTQD"
     gene            8038..8226
                     /locus_tag="PLY93_02250"
     CDS             8038..8226
                     /locus_tag="PLY93_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68332.1"
                     /translation="MVESPNETAEKAAVAPPKTNRRTESNASWLQQVKTDDSLRLEAE
                     MVTLELKLFEMKSLPSVS"
     gene            8440..>8995
                     /locus_tag="PLY93_02255"
     CDS             8440..>8995
                     /locus_tag="PLY93_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRP68333.1"
                     /translation="MSASNKKRRRTPVKDIEPARVSSDDPHHLGILIKKLRLQNEKLK
                     KMIQVSPTAMMVVEAHTGALTEFNRAWSDILGVDGESFLQTPGFFKTLFCNAEEYKLV
                     EHALRTKGTIVDQLCQMEALSRHRKTVQLYGTTFDRNGIQYYLLNFCDLTDVISNERT
                     ARSFEQKIRNLIRALDASSLVSVTD"
BASE COUNT         2502 a         2245 c         2017 g         2202 t
ORIGIN      
        1 cgaagaaata tcgcgtttgg cagcgagctt tccgcattta gagaatgcgc cgcgtatgct
       61 cacgcgcaac gaactcgaat cgattttatt ggcggcgttt taatgcgagt acaaaatata
      121 cctttattgt ggattttatt tttagcaagc gcgatccccc tctttgccaa ctcgcccgac
      181 cgtgctttct gcgataaaat tttaggtgag aacaaacgct tcatcgactt catcaacgta
      241 ccgatctcaa actttgccga tttagaggag ctggcggatt ttagaaacgc ggccttggag
      301 cactacactg cgcataaaga atttctctcc ggccattacc ctaaaaccca tgcacacatt
      361 cggaattcgc aacggatact caaagattta tactacaaag ttttaggtaa atattaccaa
      421 aaagatgcgc gcgaaatgct cgaacgtaat tcggcgatga tcatcgaaac gcgcgacgtg
      481 cgcgcaaagc atctcatgaa gttgggttat cgcgacctca aggtggccga catctacaaa
      541 caaaaaggtt acaaccacaa catccatctt tactcgacaa aaatttggtt ttatatcgat
      601 gcgattaaat acgcacggca agcgaagcgg tacgcgctcc tcgccatcat cgagagccaa
      661 acaccgtacg ccgacaaaga cgactttaag cagcagacca tcgacgacca cttcaacaaa
      721 cccgagcccg atgagaaacg cgattttgac cgcgtgcaga ctaaactcat caatttaata
      781 caccgtaagc tggtgaacga taattacaat tttttctttc atcttttcga taactatggt
      841 tatatcaatt cggagaagcg caaccagtac gatgatttta tttcacagac tcaaaaataa
      901 tgttgacata tcaagtataa atatagaccg attactcctc aaatatgctt tacagtagtg
      961 ccagtaaata tgccgtgcgc gccctcagtt acatgaccga atgcatcgac caaaccgtgt
     1021 tcactgtcga cgaactggcc aagggagcca acgtacccaa accgtattta agtaaaattt
     1081 tgaaacagct cgtcagcgga aaaattctca agtcgagtaa aggtccgggg ggaggatacc
     1141 aattcgcgcg ctcggtcgac aagattagtc tttacgatat taaagtcacc atcgatggca
     1201 ttcaagaatt tgtcgattgc gttttgggtt tagacgcctg caacgatgct gccccctgcc
     1261 ctgcgcactt catgtggaaa gaattgcgtg cgcaaacaac ggtggcgctg caaacaacga
     1321 cgcttcctgt cgcgcaaaga attatgcacg agaaggcgct ccatcccgaa ggcgcaaaaa
     1381 aacgcacgca aaaaaaacgg ttacaatagc cagtggaacg gctttagcaa aaacgcgcgc
     1441 aatcctcctt tgagttcatc tttcaccgcg agacggctac ccgaataaag ttcgttcaac
     1501 actggattaa acctgcctgc cggatgacga aacgcaagca tcgtctctaa attgagcacc
     1561 atgctacggt aatcaaaatt tgcactgcct gtcaaaaccc attcgtcaat cattaacagc
     1621 tttgtatgcg aaaagcgcgg taaactttca ttgcctcggt acaaaaaaac gcgcgcccct
     1681 tttttttgca aacgctttgc taaagagaat gaaacggcgt cgatccacgg taaatcggat
     1741 ctttcggaca aaataatttc tacgcgcacc cccttgcgta atttctgttt tagtacgcgc
     1801 cgccaaaatc catacggcac aaaataaggc acggcaatca caatacgttt ttgagcgcgg
     1861 agtaagcggt gcttcatgta acgcgaaagt aaatacgtga gtacgcggcc gcggtgagaa
     1921 aaaataacgc gattaccggt gcgcgttaaa agccgcgccg cccatttctc gtggcgtgcg
     1981 cggcggcaag cgtcttcaaa ttgttccatg atgggattta tcaaggcacg ctctttcacg
     2041 actaacgcga tgtctaacca acgcgcttta ccaaaaaatt ttctcgagca ttcgcgcatg
     2101 atattaaaac caccggtgaa cgcaatggtc tcgtcgacga taataatttt acgatggttg
     2161 cgtcgtataa ggaagcggag ccactgccag cggaaaattt tcaaacgatg gtggatgaat
     2221 acgtggacgc ccgcctcgcg caaacgcgca atgacgcttc tgggtagttc aagactaccc
     2281 accgcgtcca caaccaaacg tacatatacc ccgcgctggg cagcggcagc caacgctttt
     2341 tcaaaccgcc gacctatttc gtcgtcctgc caaatataaa ccatgaacgc aaccgtcatc
     2401 ttggcttgct cgatagactc taatgctttt ttgtagaaga cgtcgccttc ggtaaggata
     2461 cgggtagcgt tcaacatctg tttactacct ctttttttca tgttatcttg gcaagagtga
     2521 tacacaatta tattcgatat gcagacagaa atgctctatc ttacatgcat taaactgtgg
     2581 taaccacatt gatctctccg cttttttacg cgatttgcac gccgctgccc ctgtaattaa
     2641 gaaaggactc aaagaagttt tggcaaatca atgctataag aatctcaaaa gttcttgaaa
     2701 gagtatatcc cactcaaaga ctcactctac aatggctgaa cgcaacgaaa caacgacaat
     2761 ctctgacgac atgattttca aaggctccat agaaaccgac catgccatgc ttatccaagg
     2821 ccgcgtttca ggcaacgtga ttgcacgggc acgcattcaa atcgacgaaa aagccgacgt
     2881 acaggccaac gtgagcgctc gcgatttaga aatcctcggc tcactgcaag gtaacgtgtt
     2941 gaacgccgag agcgtcacac tgcaaccgag cgcgcgttta accggagaca tccaaacggc
     3001 acagctccaa atccaacgcg gcgcaaaaca caacggcaac acgagtatgc ggtaaagcat
     3061 gtttcctata aacacggtaa tccagttttt caaggcgaca taatacggtg gatttattca
     3121 caaagtggta tggatttctt tacgaacaaa aaaatagttt tgcagaaacg atttaccacc
     3181 gttttttaga acgcctcgac acttatacgg aaggccgcct acccgacgag atcaaacgcg
     3241 agcacgtctt gcgctacctt ttgccgtctg ttcaccttct taaaagccca tttgtgtttc
     3301 ccgacatggc acgcgccgta tctcttatac gcgagcatgc ccccgagaaa agtagcgact
     3361 ccggtttttt acaaaacagc gcaaaaaaga ttgttatcta tggcgacaga gatgccgacg
     3421 gcgttacctc cgccgcgtta ctgactcttt tttgccgcga acaactcggt atcactgcac
     3481 aaaatttaac gacgctcgtg ccacgcgaag acgacaagta cggtatcgtc ggtggtgtcg
     3541 tccagcgcat catcgacatg aagcccgatc tactcattac tctcgattgc ggttcgtcga
     3601 accgaaacga acttcaacaa atccgccaag cgaccggttg taaaaccatc atcatcgatc
     3661 accactttat tcctcccgat cccaacgatt ttcccgacgt tgaggcgttc atcaacccca
     3721 agcggttggc cgagtccgaa ttcgcacgcg attattgcac ggcggcgatt gtgttgcgtt
     3781 tcgtgcaagc aattcttttt tcgtacacac ggaatttcga ccaagaaatt tacctccaca
     3841 ccgcacaggg agaaatttat ttaaaaaatg gtgttcaaat tcctaagaag gatttacacc
     3901 aaattcaaaa gcattattcg ctgcgtgtca aatctcccat ttccaacgaa tgccatgatt
     3961 tagaaaaaat ttttgctcac accgcgcgtg aaaatttttc gatgcaacgt cttttccact
     4021 tttttcaaaa agcccccgac gcgttttctc ccgttgaaat ttttaacgcg gtggaatcgg
     4081 cgagttaccg taaagtgcac gccattctcg cgccgtacat ccccttggca tcgattggcc
     4141 ttgtcgccga cctcatgccg atttacgccg acaaccgcat tctcgtacgc gaagggttgg
     4201 acgtcattac gcgccaactc gacacattgc cgatcggact tgttgctctt atcaaaaaac
     4261 tcaacctcgc ctcttcaatt caagaacagg atttaggttt ttctctctgc cctgtcatca
     4321 atgctgcagg ccgcatgggg aacgccgcgc ttgcgctcga tgcgcttgtt gaaaaggatc
     4381 cgctcaacgc cgccaaagcg gcgcacgcgc tcatccaaac caatgatgcg cgcaaagagg
     4441 cgtcgcaagc ggcgctcaca cttttagacg atgcgctcga ttcccgttcg acgcactctc
     4501 cgattgccgt cgcggtacat gcgcactttc atcgcgggat ctctggactt atcgcctcta
     4561 aaattgcaga gaaactggga aaaccatcgg tcgtcatggt gcccgacggc gacacatacc
     4621 gaggttcggt gcgcgcgtat aaaaacgaga acgtatacag cttgcttgag tcgctgtcgg
     4681 cgcatctcat ccaattcggc ggccacaaac aggcggcagg cttttcggtc gccgaagaaa
     4741 atgtaccagc gtttatcgac gcggtatttg cgaaggcaag cgcgctttat ggcaaggcag
     4801 agtctaatgg cgacggcatg atgattgacc ctttcagaag ttttgatccc cccttagaaa
     4861 ttgatatttc acgcctacgt accgctcttt ggaatgaact cttaacgcat gcaccgtacg
     4921 gggtcatcaa ccctcatccc attgtctcca ttcttgaagc gggtaaagtg gaaattagcc
     4981 ctttgggaaa gacaggcgca catgcaaaaa ttaaatttgc cccacccgcc gacgcaagta
     5041 tcgagggggt ttggtttttt cacggtggcg cggcagagag tatcgctaca cgggaaaatc
     5101 ttatagtaag cggcgaacct caagtaggca aatttatggg acgcgttcaa taccggttaa
     5161 agattacgaa gatttcatga gctaagaaaa caatcttgaa agcgtaccat tcaagatttc
     5221 taccgatacc tttaaaattt taaaccaagg cagcatctat tgtacaacaa tggatagcgg
     5281 tttctttaaa gactgagaaa ttccgaagga atgcgagggc ggttttttcc gactactcac
     5341 tcttgggatt gcctgcgcca agtagtgtag tttcgcgcaa tgcgccgtaa gaagggatac
     5401 tcaacgggtt aacgaggcag cggccaaatc tcttatactt cagatttgaa tcggctatag
     5461 gataaaaagg tctttttatt tattgaattg acgcgatgag cgctcaagga tgtcgactgc
     5521 tgcatgaatc tcaaccgcta tgtcccgctg cttttattgc tggggttttt cgcctgccgc
     5581 gaaaaaccac cgatccgctc gtctgattat atggcatccc tacccgaggg tgaagtcaaa
     5641 cgcaagctca tcatgggctg cacttcgtgc caccagatgg gtgtcccggt cgcgttgcgt
     5701 aacacgcgcg aagaatggct gggtaccatc aacaagatgc tcaaactcga tgaagacctg
     5761 aagctgcatc tgattccgct cagcaaagaa gacctggtcg actggctgac aaagcatggt
     5821 agcggaccgc tcgaaaaaag gaatatcaaa atctacagcg atgtgccgac cgcgaaagcc
     5881 gattatgagg agttctctct cggcaacgtg aaaggctttt atcacgatgt cgcgatttct
     5941 ggcgggcgcg tatttgtcgc cgattatttc ggcaataaac tctatagtat cgatctgaaa
     6001 acccgcaaga cagaatcgta tgatattccc gtaagttcaa aacccggcaa acccggcggc
     6061 gcgcacgcaa tcgacacgac gcgcgacggc aaactgtggg taacgttcac caagtccgaa
     6121 caggtaatgc agttcgatac caagacgaac aagttcagaa tctataacgg atttttgcgc
     6181 gacggcaatg tgcaatattt tgttctcgat gccgaccgca atatctatga nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnt ctcgaaggaa atcctctcgc gcgtgaaccg cgattcggga
     6301 gaaacaaaaa tattccagac tacccgcacg cagaacatgg acgaagccgc ggtgcatctt
     6361 tacgcagcgg tcgccgacag caagggcaga ctgtggtata cggaaacaca cggcaaccac
     6421 atgggttttc tcgacccggc gaccggcaaa acctgggaga agccgatggc attagcatgg
     6481 gctggcccaa agcggctggc gatcgacaag aacgacatgc tgtggattcc agaactcgcc
     6541 accggaaaaa ttacacttta tgatacggtg aaagaatcaa ttgcgggaca gatcgaattg
     6601 ccgattaaag gcgactacat ctacgcagtg cgcagaaatc cgtacaatgg cgatatgtgg
     6661 gcaaacggtt cgggatcgga cagcctatac cgcatcaacc cggtgacgaa ggccgtgcgg
     6721 gtataccgtc tgccacggct cggtactttc acacgcacag ttgcgttcga ccaagagggt
     6781 aatgtttata cgagttatgc ttcattgcct aacaagcaca cacaaatgcc gcacgaaagc
     6841 gctgtgattt tgaaggtgac gcctaaagat tagcagtttc ggaaaatcat ccgattcggc
     6901 gtatcaccag cagcgccagg ttgtagcccg ctcaagcccg cagagtctga acatccactt
     6961 ggaattattc aagcctgcga ggtaagaccg tcccttacgt ctaatcttgc gttaggctca
     7021 tgccctgtac gggctctatt gaagctttct tcttagttct gagtttgaaa ggtacggttt
     7081 tgtatccccc attgggctta ggtacaatta cgtcaaaatg taaatgcgga tcagttgacc
     7141 atcccgtatt gcccgatgaa ccaatcttct gacctaattt gattttatcc ccgggcttta
     7201 ctctcacacc gccctttctg aagtgcagat aacgcccaat agagccgtct tcgtgtttga
     7261 tataaatata atttgcttta tcgcctaatg cagggtcatt gccccctact gaaaattttg
     7321 attctgtttc ggtaacaacg ccttcacgag cggcacagat ttcagtacct tcgggcattt
     7381 tgaaatctaa tgcatatgcc cagtcgcctc tgtgggcgcc gtaactgttg aagccattgt
     7441 ctatgcgaaa agttttaccc tttacccaca ttaggttata tataccatta tgctttacag
     7501 gtttattgct ttggtcagat aaaccaaaat ccgcatgcag ataccacgat aaatttctaa
     7561 agtagaaagc cttgttggca tctatgcgag aaatctgaaa gagcttgagc tctcttgtgg
     7621 ttgaggcagg taacttcatt ttcacgggca aagctttttc gcttttcatg ttatctaact
     7681 gcatgttgac ttctacaatg aaatttttgg tttcacggtt tattgccctg aattcgacta
     7741 tagcatcatc agcgctctgg tataataata gctctgcatt ggcgccgctt gccttaattt
     7801 gctgaatatt tctgaattgt tgtccccaga cagtatgtac tgtcaaagag cctaaaagaa
     7861 aaaaaactat cttgcgcatg agacagcata ccgcggttgc atgcgggtac aagcagtttt
     7921 tcaagggtta gacattttgt tccttatcgc gttgtcgata cacagattca gatattgagg
     7981 gttttgcatg gggcgatgga ttagccaaaa gagttctgaa agtaatttct ttgacgcttg
     8041 gtagaatcac ccaacgagac tgcagaaaaa gccgccgtag ctccaccgaa gacaaatcgc
     8101 cgcacagaga gcaacgcgag ttggttgcag caagttaaga cggacgactc gctccggctc
     8161 gaagccgaga tggtaacgct tgagctaaaa ctttttgaaa tgaagagctt accatcggta
     8221 tcgtagggga tttgtccagc atgtactaat aatgtctcgt cgcgattaaa tataggtaag
     8281 taatcgtaac ctatcagctg cggattgaaa acgacgctta attttcaggt tcaaaagcta
     8341 cgtacgtaaa ctttcgtacg tatgaaagac gatatattaa aaaaaacact gtacacgcaa
     8401 taaatactac tggctctata tgttatagag ggtacgtttg tgtctgcatc caataaaaaa
     8461 agacgaagga ccccagtaaa ggacatagag cctgcaaggg tatcatcgga cgatcctcac
     8521 cacctcggaa tcctgataaa aaaacttcgc cttcaaaacg aaaagctgaa gaaaatgatc
     8581 caggtaagcc cgactgccat gatggtggta gaggcccata ccggcgccct taccgaattc
     8641 aaccgcgctt ggagcgatat attgggcgtc gatggcgaaa gttttctgca aacgcccggt
     8701 tttttcaaaa ctctgttctg caacgccgaa gagtataaat tagtcgagca tgctctcaga
     8761 acaaaaggca cgatagtcga tcaactatgt caaatggaag cgctgagccg acacagaaaa
     8821 accgtacagc tctatgggac aactttcgac cgtaacggta tccagtatta tctcttaaat
     8881 ttttgcgatt tgacagatgt aatttcaaac gagcgtaccg cccgcagctt tgagcaaaag
     8941 atcagaaacc tgatccgcgc actggatgct tccagtctcg tctcggttac ggacg
//
LOCUS       DAOMPD010000037         8805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3415_length_8805_cov_2.840914, whole genome shotgun sequence.
ACCESSION   DAOMPD010000037 DAOMPD010000000
VERSION     DAOMPD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_8805_cov_2.840914"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1570
                     /locus_tag="PLY93_02260"
     CDS             227..1570
                     /locus_tag="PLY93_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68334.1"
                     /translation="MKVHQNMPEDSFLYMEFQGSNQMRWAADYMKAKAEGRYSGVCVN
                     INYPPEEMNYGNNIQCGAVSILRVGGVKPDYFWDAFWDDLLFFSWHVPDMGLYANNFT
                     SWYHFVNLLKTNEDGNRLVTNNYNKFDGYGYNATYGFPNLGIDYTLATFMNNAQMSVN
                     LPGCTDSTCSEWQGISTAYRANPAVDYKQNGSTTPVSGGSGGKKTEQADGMNYNCFSD
                     WTILGNCPDNGAEVDGTTQVPNVVPGGGHILSGDEDWVIYEPATNAATFYYNEMFLEG
                     LQSKNHSLQTGPIVGRYYTIPGAELLYFSVVQHWMGDMAQQAHVWSTIGYNHGDYESY
                     ADETYGDRLIGQSVANRNLEDYVESQAYANGWQNRYNQPVGNIDRFIMEQAFRAYHIH
                     NRAGYNFMVSSNKSGVWMPALVWAVRNSTAAMAIVNEKAVLDLRRCRNSAVCTEF"
     gene            1653..8192
                     /locus_tag="PLY93_02265"
     CDS             1653..8192
                     /locus_tag="PLY93_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68335.1"
                     /translation="MILYNRMLNNKKPWYCGLLHGKYALFLLLSLLWNACAPNELAFQ
                     KFEPQSDTALSDLFNETPAIRAAFTTVDPTEFNNRMDKLLRKDPVMGVKLMHALGSLA
                     YQKPGDRPALPALVDDVSSTVDRFARFYMATEARKDEYNQAMDVISALLDIDPSAITD
                     GSYLGARVIKYLGENKWLYPLSDPNAPANLAFANEFYFPALTTWQTCDDPGSVFLNAK
                     PRSHETDLEGRDTLWSMYDMLRTGYCIYANRTGPRVNVVTSDTGVRDAKIEAVVTDGV
                     VTALNVIDPGSGYNTVTPILLIDSTPGPGSGATGFATVGVIQKPVVAVANGGAGYSTP
                     PTITVSAADCPDTSMKFGATVSGGAITAIDVQDVTFGCTNPSPTLIISPPDAGGTPAT
                     VTVTMDSSQKGIMSATLTTGGSNYVRKNDDTPGAYMYNATKVMKNSGNVELRIRNLIN
                     DLQNNLQDFTDAEKKIADWTVNRSSPANQAQVDLVDYVIEHVYPITQKDFVFVNGKEV
                     LDREAEHVSMLNPGGSVATKDEYLTEWLMKSLAEDAPRLDDLENFTALDTNSPLYKFG
                     KDMVQNPTYGLNRVTQRYPKPKLKGHLYTGFNFDPGCSGATPSCDFKGLLASDGYIKK
                     LLSASSTQKYKNPFRTYMSTRVNALVDVPLTYNNELYFESALKNLYYHLLERYYDQTT
                     KKWAFSPEDAATLFASDPNGTERNLQSYIGQMQYSMRNMAILDKAGRKPGDVGFDNVP
                     YLTSFIYTIAAANGFVDPVNAPAQLTLKTCLTSMGATDIADDGVRTISMPLGLPAMNS
                     QVTNMAIADGIPASSRNDQPYSTSLQMFAFELLSPGRFIPRADTDIHMSDLVNGKFRG
                     RFSPHQGDIESTKQKTSNWMVSEFALSAWEGYGPYSVKGKAANGSKVKYENDFYTDGY
                     RSKIATGAGDYATIAMGVNGGEGAGTYAGNGNYHIYEMIYRPLNSGDPCWADAQGATY
                     GYARYGYIRPQNNTSYSNVTNCLGWAKIRLDFDTRDEAIRANIEWVLKYKKYIFVIPM
                     YGYTNMWTLWNNGAAFAVFSTIHGNGLWGITTGRRACSAQTCNGQWLRSGVSMGANGD
                     GLIANSGNGFVTEASAGGQPGVTPITKTVRMGAQSYQAGDSMVLMEISFNQWGLTGLL
                     VNVFQQIWDSMGDGPITPAMVMDNFDSVLALADAVYKTDDIMAGAVATHNATPTMDSF
                     QKFYDTFFPTDETQCLGGNLVPGAGGGPDLFENFKNGCGITARELPPLPKVNPSMNGT
                     CSEFVQTACIKYPKTYKADGSVDTWYLYAGPSDGKLTGMLTPLIMLFGTMHEDGQVMK
                     VSSQVNDSIPGGFTPVRYNATALGAGEDRDSVTIRNFCTDGYPGCNSADLGYRDQLDT
                     LFTGLASLNDSKMTGGVGANKNLPFYNTTPGSEALTNLITETAPGLRNGFVPKLTSNK
                     YANTAYIDPLVRDLEGIIADNVRKFEDGFKITSGTEISGDYKNKDRLRYFATKNVINP
                     EIETSSTTTGIAHAKYHGLEEGDRVYLTGSVPPGLQTNNPSALDPLKAYFVVAPVTQD
                     TFKLSATRGGAAIAFSPASSWKVHPWLFRTNRFSASVNGVNLNGVPINMMKQFISYLR
                     GLTDDVQVVNAIKAAIPVINNYLQHVQNSSTTLTLSDQDIDHIVDFVHDKNSTDEYSI
                     DTFLDVLVSTKMDDLNTLRSFNFEQFKELGSYENVFEDINDKVSKYFDINLKKDILYS
                     PFMLGEPTCSGGSQNGFYDHNEDGKWDPGTFTFISQAYVPLKTFTDTANVGGSCSITT
                     KVPNIYHGSYYMLDMGGVARNMKRGVEDVTSTDIDATLNWLYGRNLTGAPSDVVYGPN
                     PQNGSKVECYIKEQSFCAAGGCIDFDKEVTAVKNLMFCNLYEKRVDEPHYDRNGNNII
                     EAGEFTDVSGDGNYNDKDSAGAVNIRKMIHYYLEGNGKPKGHPDYFGGYVSFYNENIQ
                     PDLLLSPDRNYIHFAAKMIPDLLAPTRCSADGLSCAGNSDYVTKDLVQARNALYKTTN
                     FTGAELKAVKNVIGNLLYDVDTNEYTRLFERIGPSLVGILREFEGDYEDLLNTGLEGF
                     KPDGFMTYFSTSLTNKPPYTSLDILSDIRTLFNTRTMRCYPPNTGDSGGYSADSYCRK
                     FRTNDTFWGQFGRLMQQFSTAAYNKYKGQWESEIPAPYFDKLVGIFE"
     gene            8220..>8805
                     /locus_tag="PLY93_02270"
     CDS             8220..>8805
                     /locus_tag="PLY93_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68336.1"
                     /translation="MKHFKKYVVAIAVFAGTPLLADIAESFGLGPRMVGTSGAGVASI
                     NDWTATFYNIAGVTAPYTERSAFGNQDASQTAAGKIKLLRDDGSLQANEKGNQDTIDK
                     KLAEEMTGENKPHHQLGMNYLFQASLPTLSPTRTNTKIAQNVGLATKNMVYGAVQMGL
                     VFDTRTLFNTPKNMPIRLGLALSLRDNGTIATVND"
BASE COUNT         2435 a         2018 c         2086 g         2266 t
ORIGIN      
        1 ttttcagtac gcttaacccc agttctttcg caaggcaaag tggttgcata ccaaggttcg
       61 ttctcagggg tggggcgctt ctccagtgca gtccaagggg caacgctcat acccattcaa
      121 aatggaagct tttacgtaaa ggtacagtat gccaattaaa aagagagccc atctttttat
      181 atatatttcc attttgagcg catgtatacc gcttcatgca ttcaccgtga aggtacacca
      241 aaatatgcct gaagatagtt ttctttatat ggaattccaa ggctcaaacc aaatgcggtg
      301 ggcggcggat tatatgaaag ccaaagcaga ggggcgttat agcggtgtgt gtgtgaacat
      361 caactatccc cccgaagaaa tgaattatgg caacaatatt caatgtggtg cggtgtcaat
      421 tttacgggtg ggcggcgtta aacccgatta tttttgggat gctttttggg atgaccttct
      481 cttctttagt tggcatgttc ccgatatggg tctttatgct aataatttta cttcttggta
      541 tcattttgtg aatctcctga aaacaaacga agacggtaat cgccttgtaa cgaataacta
      601 taacaagttc gacggttatg gttacaatgc cacgtatgga tttcccaatt taggtatcga
      661 ttataccttg gcgacattta tgaataatgc acaaatgtcg gtaaacttac cgggatgcac
      721 cgattcgact tgttccgaat ggcaggggat atcgacggca taccgagcaa atccagcggt
      781 cgattacaaa caaaatggat cgacgacacc ggtttcaggg ggaagtggtg gcaaaaaaac
      841 cgagcaagcc gatgggatga actacaactg cttcagcgac tggacaattc tggggaactg
      901 tcctgataac ggcgcagaag tagacggcac gacacaggtg cccaatgttg tgcccggcgg
      961 cgggcatatt ctctcaggtg atgaagactg ggttatctac gaacccgcga ccaacgctgc
     1021 gacattttat tacaacgaaa tgtttttaga agggctacaa tcaaagaacc actcgcttca
     1081 aactggcccc atcgtcggta gatattatac cataccaggt gcggaacttc tttattttag
     1141 cgtcgtgcag cactggatgg gcgacatggc acaacaagcg catgtttggt cgaccatcgg
     1201 ctataaccac ggcgattacg agtcgtacgc cgatgaaact tatggggatc gattgatcgg
     1261 gcagtcggtg gcgaaccgta acctcgaaga ctatgtcgaa agccaggcgt atgcaaacgg
     1321 ctggcaaaat cgctataatc aacctgtggg taacatcgac cgttttatta tggagcaggc
     1381 gttccgcgca taccatatcc acaaccgtgc agggtataac tttatggtta gctcgaataa
     1441 aagtggtgtt tggatgcctg cgcttgtttg ggcagtgcgc aactcaaccg ccgcgatggc
     1501 gattgtgaat gaaaaagcgg tgttagactt gcgccgctgc cgcaattccg cagtttgtac
     1561 tgaattttag cgggcgtaca ggctcctaaa cggcgtatac acttttttgg cacaacaatg
     1621 actttaatcg tcattaccta ttaggggttc ctatgatttt atataataga atgctaaaca
     1681 acaaaaaacc atggtattgc ggcctactcc acggtaaata tgcgctattt ttactcctct
     1741 cactcctgtg gaacgcctgc gctcccaacg aattagcgtt tcagaaattt gaaccccagt
     1801 ccgacaccgc gctttccgac ctctttaatg aaacccctgc cattcgtgcc gcgttcacca
     1861 cagtcgaccc cacggaattt aacaaccgca tggataaact gctccgtaaa gaccctgtta
     1921 tgggggttaa gctcatgcac gctttggggt cgttagcgta ccaaaaaccc ggtgaccgcc
     1981 ctgcattgcc agcgcttgtg gatgacgttt catccacagt cgaccgtttc gcgcggtttt
     2041 atatggcaac cgaagcccgt aaagatgagt ataaccaagc aatggatgtc attagcgctt
     2101 tactagacat cgatccttct gcgattaccg atggctccta tttgggggcg cgggtgatta
     2161 agtatttggg agaaaataaa tggctttatc ccttgagcga ccccaacgct ccggcaaatt
     2221 tagcttttgc aaacgagttt tacttccctg cgttgacgac atggcaaact tgcgacgatc
     2281 caggttcggt gtttttaaac gcaaaaccgc gttcgcatga aaccgattta gaaggccgtg
     2341 ataccctgtg gtctatgtac gatatgctgc gcacaggcta ttgtatttat gcaaaccgca
     2401 cagggccgcg tgtcaatgtt gttacttccg atacgggtgt acgcgatgct aaaattgaag
     2461 cagtggttac cgatggagtg gtcactgctc ttaacgttat cgatcctggt tctggttaca
     2521 acacagttac ccccatcctt ctcattgact caacccccgg cccaggttcg ggggccactg
     2581 gctttgccac cgttggggtt attcaaaaac ctgtcgtcgc cgtcgcgaat ggtggtgctg
     2641 gatattcaac ccctccaacg atcactgtga gcgcagccga ttgccccgac accagcatga
     2701 aatttggggc aacggtgagc ggtggggcta tcacagcgat tgacgttcaa gatgttactt
     2761 ttggttgtac caatccgtcc ccaacactca tcatctcacc acccgacgca ggaggcacgc
     2821 ccgcaaccgt tacagtgacg atggattcat cgcaaaaagg aattatgtca gcgaccttga
     2881 caaccggtgg aagcaattat gtacgcaaaa acgatgatac cccgggcgca tacatgtata
     2941 acgccacaaa ggtgatgaag aatagtggga atgtagaact gcgtattcgt aatctcatta
     3001 acgacttgca gaacaacctc caagatttta ccgacgcaga aaagaaaatt gccgactgga
     3061 cggtgaatcg gtcgtcacct gcaaaccaag ctcaagtaga tcttgtcgac tatgtcattg
     3121 aacatgtgta tccgattacc caaaaagatt ttgtatttgt caacggtaaa gaggtgttag
     3181 atagagaagc agaacacgtt tcaatgctaa atcccggagg gtcggttgca acaaaagacg
     3241 agtatctgac agaatggctt atgaagtcat tggctgaaga cgcgccgcgt ttagacgatt
     3301 tagaaaattt tacagcgtta gacaccaaca gtcctctcta taaattcggc aaggatatgg
     3361 ttcaaaaccc tacatacggt ttgaaccgtg taacgcagcg ctatcctaaa ccaaaactca
     3421 aagggcacct atacacaggc tttaatttcg accccggttg ttcaggagcg acaccgtctt
     3481 gcgattttaa aggccttttg gcgtcggatg ggtatataaa aaaattgctc agtgctagct
     3541 caacgcaaaa gtataaaaat ccattccgta catacatgag cacaagagtg aatgcacttg
     3601 tggatgtgcc tctaacctat aacaatgaac tgtattttga atcggcatta aagaaccttt
     3661 attaccacct tttagagcgg tattacgatc aaacgaccaa gaaatgggca ttttctcccg
     3721 aagatgcggc gacacttttc gccagcgacc ccaatggtac cgaacggaat ttacaaagct
     3781 atatcggcca aatgcaatat tcgatgcgta atatggccat tctggataaa gccggacgta
     3841 aaccgggtga tgtgggtttt gataacgttc cctacttaac ttcatttatt tatactatcg
     3901 ccgccgccaa tggctttgtc gatcctgtca atgcaccggc acaattaacc ttgaaaacct
     3961 gtcttacctc gatgggggca accgatattg ctgacgacgg agtgcgtaca attagcatgc
     4021 cattgggctt accggcaatg aattcacagg tgacgaatat ggcaattgcc gatggtatac
     4081 ccgcttcgtc gcgtaacgat caaccgtata gcaccagctt gcagatgttt gcttttgaac
     4141 tgctgtcacc cggccggttt atcccccgcg ccgatacgga tatccacatg agcgatctgg
     4201 tcaatggtaa attccgcggt cgtttctcac cccatcaagg cgatattgaa tcgaccaaac
     4261 aaaaaacttc caattggatg gtctctgaat ttgcgctaag tgcatgggaa ggctatggtc
     4321 cttattccgt aaaggggaaa gcagccaacg gcagcaaagt aaaatacgaa aatgattttt
     4381 atactgatgg ttaccgctca aaaattgcaa ccggcgccgg tgattatgca acgattgcaa
     4441 tgggagtgaa tggtggtgaa ggggctggta catacgctgg caatggtaat taccacatct
     4501 acgaaatgat ttaccgtcct ttaaattcag gcgatccttg ttgggcggat gcacaaggcg
     4561 ccacgtatgg ttatgcacgg tacggttata tccgtccaca aaataatacc agctactcca
     4621 atgtcacgaa ttgcttgggc tgggctaaaa ttcgtttaga cttcgacacc cgtgacgaag
     4681 ccatccgtgc caatatcgag tgggtgttga aatataaaaa atatattttt gtgataccca
     4741 tgtatggtta caccaatatg tggacgcttt ggaacaacgg cgctgcgttt gctgtttttt
     4801 caacaatcca cggtaatggc ctgtggggga tcacaacagg ccggcgggct tgtagcgcgc
     4861 agacgtgtaa tgggcagtgg ttgcgttctg gcgtgagtat gggagctaat ggtgacggtt
     4921 taatcgctaa tagcggtaat ggtttcgtga ctgaagcctc agcgggtggt caacccggag
     4981 ttactcccat caccaaaaca gtgcgcatgg gtgcgcagtc gtaccaagca ggtgactcca
     5041 tggtgctcat ggaaatttct tttaatcaat ggggtttgac cggcttgttg gttaacgttt
     5101 ttcaacaaat ttgggacagc atgggggatg gtccaattac cccggcgatg gttatggata
     5161 attttgactc ggttttggcg ttggccgatg ctgtttacaa aaccgatgat attatggcag
     5221 gtgcggttgc gacgcacaat gcaaccccca caatggatag ctttcagaaa ttttatgata
     5281 cgtttttccc aaccgatgaa acccagtgcc ttggtggtaa tttagttcct ggtgcaggtg
     5341 gtggtccaga tctttttgaa aactttaaaa atggttgcgg tattactgcg cgtgaattac
     5401 cccctttacc taaagtgaac cccagtatga atggaacttg ttcggagttt gtacaaacag
     5461 cttgtatcaa gtaccccaag acatacaagg ccgacggtag tgtggacaca tggtatcttt
     5521 atgcaggccc ttccgacggc aaactcaccg gtatgctcac cccattgata atgctttttg
     5581 gcacgatgca cgaagatggg caggtgatga aggtttcgag ccaggtgaac gactccatcc
     5641 ccggtggttt tacgccggta cgctacaacg ccacagcgtt gggcgccggt gaagatagag
     5701 atagtgtcac tatccgtaat ttttgtacag acggttatcc agggtgtaat agcgccgatt
     5761 tagggtatcg agatcaatta gatacgctgt ttacaggatt agcctcgctc aacgactcca
     5821 aaatgacggg tggggtgggc gctaacaaaa acttaccttt ttataataca acaccgggaa
     5881 gtgaagcatt gacaaacctc atcacagaaa cagcccctgg tttacgcaat gggtttgtac
     5941 ctaaattgac aagcaataag tatgcgaata ccgcgtatat cgacccactc gtccgtgatt
     6001 tagaaggtat tattgccgac aatgtgcgca aatttgaaga cggcttcaag ataacaagcg
     6061 gcacagaaat tagcggcgac tataaaaata aagaccgctt acgctatttt gccactaaaa
     6121 atgttataaa tccagaaatt gaaacctcgt caaccaccac aggcattgct cacgcaaagt
     6181 accacgggct tgaagaaggc gaccgtgtgt atttaacggg ttcagtgccc cccggcctac
     6241 aaaccaacaa ccccagcgca ttggatccac ttaaagcgta cttcgtggtg gcgcctgtga
     6301 cgcaagacac gtttaaattg agtgccactc gaggcggtgc agcgattgct tttagccctg
     6361 cctcctcttg gaaagtgcat ccatggcttt ttagaaccaa ccgctttagt gccagcgtca
     6421 atggcgttaa cctgaacggt gtgcccatca acatgatgaa gcagtttatt agttatttgc
     6481 gcggattgac cgacgatgta caagtcgtta acgccattaa agcggcgata ccagtgataa
     6541 ataactacct acagcacgtt caaaatagta gtacaacgtt gacgctgagc gatcaagaca
     6601 ttgaccatat tgtcgatttt gttcacgata aaaatagcac cgatgaatac tccatcgaca
     6661 cattcctcga tgtgctcgtc tctactaaaa tggacgattt gaatacattg cgtagtttta
     6721 actttgaaca attcaaagag ttaggctcgt acgagaacgt gttcgaagat ataaacgata
     6781 aagtaagtaa atattttgac atcaatctca aaaaagacat tctctacagc ccattcatgt
     6841 tgggcgaacc aacgtgttcg ggtggctcac aaaatggttt ttacgaccac aacgaagacg
     6901 gcaaatggga cccaggcact tttaccttta tctcacaggc gtacgtgccg cttaaaacat
     6961 ttaccgacac ggcaaatgtt ggcggttcat gctcaattac taccaaagtt cccaatatct
     7021 atcacggcag ctactacatg ctcgacatgg gaggggtggc gcggaacatg aaacgaggcg
     7081 tcgaagatgt cacttcgacg gacatcgatg ctactctcaa ctggctctat ggccgcaacc
     7141 tcacaggcgc tccttctgac gtggtgtatg gaccgaatcc tcaaaatggt agtaaagtcg
     7201 aatgttatat caaagaacaa agcttttgcg ccgcaggggg atgtatcgat ttcgataaag
     7261 aggtcacagc tgttaagaat ttaatgttct gtaatcttta tgaaaagcgt gtcgatgaac
     7321 cacactacga tcgtaacggt aacaatatta tcgaagctgg tgaatttacc gatgtgagtg
     7381 gcgacggtaa ttataacgac aaggatagcg cgggggctgt caatatccgt aaaatgatcc
     7441 attattattt ggaagggaat ggtaaaccca aaggccatcc ggattatttt ggcggttatg
     7501 tttcgtttta caacgagaac atccaacccg atttattgtt aagtcccgac agaaactaca
     7561 tccactttgc cgccaagatg atacccgact tactcgcgcc aacgcgttgc agtgccgatg
     7621 gcctttcgtg tgcaggaaac agcgactacg tcaccaaaga tttagtgcaa gcacgtaacg
     7681 cgctttataa aacaactaat tttactggcg ctgaactgaa agcggtaaaa aatgtgattg
     7741 gtaatctact ctacgatgtg gacaccaatg aatacacccg cctttttgaa cgcatcggtc
     7801 cgtcgttggt cggtatttta cgagagtttg aaggtgacta tgaagaccta ctcaacaccg
     7861 gcttggaagg attcaaaccg gatggcttca tgacctactt ttcaacgagc ctcacgaata
     7921 agccccctta tacgtcgttg gatatattgt ccgatatacg tacacttttt aacacccgta
     7981 caatgcggtg ttatccgcca aacacgggcg actcaggcgg ctatagcgcc gattcatatt
     8041 gccgaaaatt ccgtacaaac gataccttct ggggacagtt tgggcgtttg atgcagcagt
     8101 tttccaccgc tgcgtacaac aaatataaag gccagtggga aagtgaaatc ccagcacctt
     8161 attttgataa attagtaggc atatttgaat aaggtgaatt gcatctttaa ggagtcgatt
     8221 tgaagcattt taagaaatac gttgtggcaa tcgccgtttt tgcaggtaca cctcttcttg
     8281 cagacatcgc tgaaagtttt gggttaggtc cccgtatggt gggtacctcg ggggctggtg
     8341 tcgccagtat caacgattgg acggcaacgt tctacaacat tgcaggcgtg acagcaccgt
     8401 atacagagcg ttctgcattt ggaaaccaag atgcctctca aacagcggcg ggcaaaatta
     8461 aacttttgcg cgacgatggt tcgctacaag cgaatgaaaa aggcaaccaa gatacgattg
     8521 ataaaaaact ggctgaggaa atgacgggtg agaataaacc acaccaccaa ttagggatga
     8581 actatctttt tcaagcatca ttaccgacgc tttcgccaac gcgtacaaat acaaaaattg
     8641 cacaaaacgt tggcctggct acaaaaaata tggtttacgg tgctgtgcag atgggcttag
     8701 tattcgatac ccgcacgtta tttaatacgc ctaaaaatat gcctatacgt ttaggtctcg
     8761 ctctttcgtt acgcgataac ggtaccatcg ctactgtaaa tgata
//
LOCUS       DAOMPD010000038         8778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3429_length_8778_cov_2.257366, whole genome shotgun sequence.
ACCESSION   DAOMPD010000038 DAOMPD010000000
VERSION     DAOMPD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_8778_cov_2.257366"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLY93_02275"
     CDS             <1..452
                     /locus_tag="PLY93_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRP68337.1"
                     /translation="MTAAAENMTGGFAQSLFAPGQAERILREDVRELYLWHAAEEMEH
                     RDLAYDVFEKVSGDYPTRVLAMVLTYGIISGYVAVGTAYLMFTDREFPWAKLPKQAWD
                     LFTQENAIGRVFLEGMIDYLRPDFHPSQHPMHQGALEYLAKFEKAAS"
     gene            539..2101
                     /locus_tag="PLY93_02280"
     CDS             539..2101
                     /locus_tag="PLY93_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRP68338.1"
                     /translation="MQNAIVFFVVSFFSLTLLFAKKTAIHDTDIRHNMPVRGALHPYA
                     VSRKNAKRFEKKFRGRFRKKPRFSALTRKHKIYLLTKKEAQSRALKKFYIKSTGKEPP
                     YAFYDTTYRQRLKRGLKSLEDLLTGYKDARLNDKLLYAIADEYPEIARLYEIGRTRLG
                     REILAVRLGSFSFNSRIADDAKKIPMLFNCSIHGDEMTSAEHCYRILYSILKNQKKYQ
                     DVLQHIDLWIVPLVNPDGAHLFWHQSYAMGRKNGRLLDGQTKTTLGRGVDLNRNFPFL
                     WESGQTKASTDKPDAWNYRGDTPASEPETQALIDLFEERRFVYSISFHSAATKLLIPY
                     TIEHVQNPEPDYASVFGHEISKTVVSHNPKKKFIATKNLYPVDGTDQDFFYFKYGTLA
                     YILETSYHNCDYRYVQYYLEGYDALWEQFLRDYANNYKFALRVVDETGNPIEAEIQFS
                     DLIWHNKEKHTTNPENGTFFRMTKSIESIQVKITADGFKTTTLAIEPLGEGYPSPQKI
                     ILPRVDSLLMEK"
     gene            complement(2122..5037)
                     /gene="polA"
                     /locus_tag="PLY93_02285"
     CDS             complement(2122..5037)
                     /gene="polA"
                     /locus_tag="PLY93_02285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRP68339.1"
                     /translation="MSGTKTKPTKKQKETLVLVDGHALAYRAHFAFAGQNLTDENGNA
                     TETTFGFFRMLARLLIDRKPSHFVLCFDPGREENPRYEMFKDYKANRPPMPDALRRQI
                     DDIQTIAHEAGFTPLIVSGAEADDTMASIVEAFYKKFDKIEIASGDKDLYNVLYNNVV
                     MLRSKMGVTQMEEIDAAWVKKKLGIEREQVPDYMALVGDSADNIPGVKGVGEKTASKL
                     MVDFKSLDALYKNLETIKPDSLKKKLTENREMAYLSQKLVTLRKDIPLELDKEDLELH
                     LSPQGVQLFRDRKMPAVATEWEKVLNALWENSSNEIAGHPPQSIKSTVAAQFCTDFSI
                     LKSAADIDAFVKNLAQVKQICIDTETTGLEIHDSNIFGISIAWHDKKKMKSVYIPLKD
                     GDAASALNADYADVDSSAVLLEAIRPLLENGKITKVGQNIKFDIQVFRKAGIEVASYS
                     DDTMLLSYLLNPNVRRHNMDDMAADHLQYKTMTFTNLVGKGKGALPIGAVPLDKLAFY
                     AAEDAEVTLALKETVLPKVEAFGLTKVYHEIDVPMAKVIADIEYDGVCIDVPFLNNLA
                     REYKKEIAAAEKKIFKLAGGEFNVASTKQLQEILFDKLEITPLRKTDKGAKSTDQSVL
                     EELKDKHPIIEPILTYRFYTKLLSTYAEALPKYVSKSTGRVHTSLSQVTAATGRLSSL
                     DPNLQNIPIKGDEGAKLRTAFIAAKGKKLLSLDYSQIELRILAHYSGDKNLIAAYAHD
                     ADIHDRATYMLFRSRFDFSNNVFAEHPKSDESGVVFEVNEKELAQMKRAPHFADFRSQ
                     AKVLNFSIVYGVTEFGLSRNLGISRAEAKELIENYFTAFPGIKKYMESAVEDARKTGY
                     AENLFGRRRFIGDLTNVNRFVREGAERLAINTPIQGTAADLIKKAMIVIREKIHREKL
                     ASRMVLQIHDELLFEVEKGEENALEALAREAMENVAKLKVPLKASGALGDNWNECKG"
     gene            5126..>6209
                     /locus_tag="PLY93_02290"
     CDS             5126..>6209
                     /locus_tag="PLY93_02290"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRP68340.1"
                     /translation="MLPDYYQFSLPTKVRYGIGLASRLGEELADFPGSRALLVTDKII
                     VSAGLAQKVIDGLKGSKITIAATYDNVPPNSEIKVVTEAAELGVKNKCDMVIALGGGS
                     VIDSAKVINLLMVKGGPLQNHMGAQLLKERLLPTIAIPTTSGTGSEATQFAVISDDAN
                     SVKLPFAEDFFMPDVAILDPEMTQSMPGRVTAATGMDALTHAIESYVGLNNNPVSDAL
                     SLYAIDLIVKNILQAVAVPNDLNARGAMLTASFLAGVAFNHGGVGIVHAISHALGGVY
                     HIPHGLANSIILPFSMAHNMESSAARYARIAEILGDSGFYPVQELNKAVARFDNFALN
                     QAVTQLSLVDDWLIKRRAESLADKLSA"
     assembly_gap    6210..6227
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6228..6846
                     /locus_tag="PLY93_02295"
     CDS             <6228..6846
                     /locus_tag="PLY93_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68341.1"
                     /translation="DNLAKIDQLVQTALDDASMLNNPESATGEDMRAIIQKAYSQNLK
                     PIAVSKSELKAARTQSGGRKLKGIFKDTAALYDILGTYFLKVQADPKLFAPLKNSKLK
                     IRFNYTAPDGAIALDGSTDDKAKQILTADAARAFIPEVEFTLSSDMSHYFWHGQVNLM
                     QALARKEVIPKGNLPRAMRLLPLLEPLYELYPQYLRERDLGKLVL"
     gene            6941..8326
                     /gene="argH"
                     /locus_tag="PLY93_02300"
     CDS             6941..8326
                     /gene="argH"
                     /locus_tag="PLY93_02300"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRP68342.1"
                     /translation="MREKLWHGRFESAPSKITEEISESISYDHALYKVDIRASKAHAR
                     ILMQMGVLTPSEFQQIEKGLATVEEEIAAGKMIFSPALEDIHMHVESRLTQIIGEVGK
                     KLHTGRSRNDQVAVDTHLFVRAALDEHKKDLRKLLVTLHSLAEKHFGKIWIGYTHLQI
                     AQPALLSHYLLSYFWQFARDTELLTFVENSVRFSPLGSAALGGVNYPIDRAQTAKELG
                     FDAPYPNSMDAVSNRDYQLNYHFGAVRLFLHISRFCEDVIIYNSTEFGYVTLGDAVTT
                     GSSIMPQKKNPDIAELLRGKTGRVNGNLQALITNLKGLPMTYNRDLQEDKVYLFDSIN
                     QVRQGLLGLTEILNHIQFNEHRVLENLSRGFALATDVADFLVREKNVPFRDAHAVAGA
                     AVKFAETHDTRLENLTAADWQKIAGYTLTFPQDFFTPLASVARRQGYGSTNPDAVKMQ
                     LDEAKAFLEVY"
     gene            8373..>8778
                     /gene="mraY"
                     /locus_tag="PLY93_02305"
     CDS             8373..>8778
                     /gene="mraY"
                     /locus_tag="PLY93_02305"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801277.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRP68343.1"
                     /translation="MLQFLLTPLQNHISWFRIFNYTTFRLVAAAITALLLTYFLGPRF
                     IRFLQKLKFGESVRSDGPESHQSKAGTPTMGGLMIMASMSFSVLLWGNLANLYVMLLW
                     VCTLALSAVGFIDDYSKSVKKIPGGMRARTKFF"
BASE COUNT         2227 a         2237 c         2006 g         2290 t
ORIGIN      
        1 cgatgactgc tgccgctgaa aacatgaccg gtggttttgc ccaatcgctt tttgcacccg
       61 gtcaagcaga gcgtatttta cgcgaagacg tgcgtgagct ctacctttgg catgccgccg
      121 aagaaatgga gcaccgcgac ttagcgtacg acgtttttga aaaagtgagc ggtgattatc
      181 ccacacgcgt gctggcgatg gttttaacat acgggattat ttccggttac gtcgccgtcg
      241 gtaccgcata cctcatgttc accgacagag agtttccatg ggcaaagcta cccaaacaag
      301 cgtgggattt attcactcaa gaaaatgcga ttggtcgcgt gtttcttgag gggatgatcg
      361 attatttacg ccctgatttc catccgagcc aacaccccat gcaccaaggc gcattggagt
      421 acttagccaa atttgaaaaa gcagcgtcgt aagacaaaaa tcaaaacggt caaatttacg
      481 cacttcgagt gcgcaaattt gactatacat taaatctctt aaagcgaaca gcgcatttgt
      541 gcaaaatgca atagtttttt ttgttgtttc tttttttagt cttactcttc tttttgcaaa
      601 aaagaccgcc atccatgaca ccgacatccg tcacaacatg ccggtgcggg gcgccttaca
      661 cccctatgcc gtttcacgaa aaaatgcaaa gcggtttgag aaaaaattcc gtgggcgttt
      721 tagaaaaaaa ccccgtttca gtgcgctaac tcgaaagcat aaaatttact tactcactaa
      781 aaaagaagca cagagtcgcg cattaaaaaa gttttatata aaaagtacgg gcaaagaacc
      841 tccgtacgca ttttacgata ccacgtatcg gcaacgtctc aagcgtggat tgaagtcgct
      901 tgaagattta ctcacaggtt acaaagacgc gcgcctcaac gacaagctcc tctatgcgat
      961 tgcagacgaa tacccagaga tagctcgcct ctatgaaatt ggccgcactc gtttaggccg
     1021 tgaaatttta gccgttcggt tagggagttt ctccttcaat agtcgaatcg ccgatgacgc
     1081 aaaaaaaatc cctatgttgt ttaactgctc tatccacgga gacgaaatga catccgccga
     1141 gcattgctat cgcattcttt attccatctt aaaaaatcaa aaaaagtatc aagatgtttt
     1201 gcaacatatc gatttatgga ttgtgcctct cgtgaatcct gacggggctc atttattctg
     1261 gcatcaatcg tacgcaatgg gtagaaaaaa tggccgtctt ctcgacggcc agacaaagac
     1321 aactctcgga cgcggtgtcg atctcaaccg taattttccg tttctttggg agtcaggcca
     1381 aacaaaagcc tcaacagaca aaccggatgc ctggaattac cgtggtgaca cccccgcgtc
     1441 cgaaccggaa acgcaggcgc tcatcgattt atttgaagag cgtcgttttg tttactcgat
     1501 cagttttcac tcggcggcga caaaattatt aattccctac acgattgagc acgtccaaaa
     1561 cccagaaccc gattacgcaa gcgtatttgg gcatgaaatt tcaaagacag ttgtttcgca
     1621 caatcctaaa aagaaattta tcgccactaa aaatttatat cccgtcgacg gcaccgatca
     1681 agattttttt tattttaagt acggtacgct tgcgtacatt ctcgaaacga gctaccacaa
     1741 ctgcgattac cgttatgtgc aatactacct cgaaggttac gacgcattat gggaacagtt
     1801 tttacgcgac tatgcaaaca actacaaatt tgcgctgcgt gttgtcgatg aaacaggaaa
     1861 tcccatcgaa gcggaaatcc aattttcgga tttaatatgg cataataaag aaaaacacac
     1921 aacgaatccc gaaaacggta cattttttcg gatgactaaa agtatcgaat cgattcaagt
     1981 caaaattaca gcagacggct ttaagacaac aactcttgct atcgagcctt tgggtgaagg
     2041 atatccatca ccccaaaaaa ttattctccc acgcgtcgat tctctactca tggaaaaata
     2101 gaataacccg ttcataaaaa tttatccctt gcactcgttc cagttatccc ccaacgcacc
     2161 cgaggctttg agcggcactt taagctttgc cacgttttcc atcgcttcgc gcgccaacgc
     2221 ttcaagcgcg ttctcttcgc ccttttcaac ttcaaacaaa agctcgtcgt ggatttgaag
     2281 aaccatgcgc gacgctaatt tttcacggtg aattttttca cgaattacaa tcattgcctt
     2341 ctttattaaa tccgcagccg tgccttgtat gggggtattg atcgcgaggc gctctgcgcc
     2401 ttcgcgcaca aaacgattta cgttcgtcaa atcgccgatg aaacgccgcc ttccaaaaag
     2461 attttccgcg taaccggttt tacgcgcatc ttccacggca ctctccatat attttttaat
     2521 tcccggaaac gcagtgaaat aattttcgat gagctctttg gcctcggcgc gtgaaatccc
     2581 caagttgcgg ctcaagccaa attcggtgac gccatacacg atcgagaagt tcaacacttt
     2641 agcttgcgac cgaaaatcgg caaagtgagg cgcgcgtttc atttgtgcaa gttctttctc
     2701 attcacctca aaaactacgc cgctttcgtc gcttttagga tgttcagcga atacattatt
     2761 agaaaaatca aagcgcgaac gaaaaagcat gtacgtcgcg cggtcgtgga tgtcggcgtc
     2821 gtgcgcataa gcggcaatga ggttcttatc tccactgtag tgcgctaaaa tgcgcagttc
     2881 gatttgcgaa tagtcgaggc tcaagagctt cttgcctttt gccgcgataa acgcggtgcg
     2941 cagctttgcg ccctcatcgc ccttgatcgg gatgttttgc aaattgggat ccaaactcga
     3001 aagccgtccg gtcgctgcgg ttacttgtga aagcgaggtg tgtacgcgac cggtactttt
     3061 cgacacatac ttcggtaacg cttcggcgta cgtgctcaaa agcttggtgt aaaaccggta
     3121 cgttaaaatg ggctcgatga tgggatgttt gtctttgagt tcttcgagca cgctctggtc
     3181 ggtggatttc gcccctttgt cggttttccg cagcggtgtg atttccaact tatcgaataa
     3241 aatttcttgt agctgcttgg tcgacgcgac attgaactct cctcccgcga gtttaaaaat
     3301 cttcttctcc gccgcagcga tttctttttt gtactcgcgc gctaaattat ttaagaacgg
     3361 cacatcgatg cacacgccat cgtattcaat atcggcgatc accttagcca tcggcacgtc
     3421 gatttcatga tagactttcg taagcccaaa agcttctacc ttgggcaaca ccgtctcttt
     3481 aagagcgagc gtcacctcgg cgtcttcagc ggcataaaac gccaacttgt cgagcggcac
     3541 tgcaccgatg gggagcgcac ccttcccctt gccgacaaga ttggtaaacg tcatagtttt
     3601 gtattgcaaa tgatcggctg ccatgtcgtc catgttgtgt cgacgcacgt tgggattgag
     3661 taagtatgaa agtagcatcg tgtcgtcgct atacgatgcc acctcgatgc ccgctttgcg
     3721 aaaaacttgg atatcaaatt taatattttg ccccactttt gttatttttc cattttccaa
     3781 aagtgggcgg atggcttcta aaagcacagc cgacgaatcc acatcagcgt aatcggcgtt
     3841 caacgccgat gctgcgtccc cgtctttaag ggggatatac acgcttttca tttttttctt
     3901 gtcgtgccac gcaatggaaa taccaaaaat gttggagtcg tggatttcaa gccccgtcgt
     3961 ttcggtatcg atacaaattt gttttacctg cgctaagttt ttaacgaacg cgtctatgtc
     4021 cgctgcgctc tttagaatag aaaagtcggt acagaattgc gcagcgactg tagattttat
     4081 tgattgagga ggatgccccg ctatttcgtt cgacgagttt tcccaaagcg cattcaagac
     4141 cttttcccat tcagtggcca ctgcgggcat cttgcggtca cgaaaaagtt gtacgccttg
     4201 aggcgaaaga tgtaactcta aatcttcttt atcaagctca agcgggatgt ctttacgcag
     4261 cgtcaccagc ttttgcgaca gatacgccat ctctcgattc tcggtgagct tttttttaag
     4321 cgagtcgggt ttaattgttt ctaaattttt gtaaagagca tcgagacttt tgaaatcgac
     4381 catgagcttt gatgccgttt tttcgcccac accttttact ccagggatgt tgtcggcgct
     4441 gtcacccacg agcgccatat agtcgggcac ttgctcgcgc tcgatgccga gttttttctt
     4501 cacccacgcc gcgtcgattt cctccatttg agtgacgccc attttggagc gcagcatcac
     4561 tacgttgtta tagagtacat tataaaggtc cttatctccc gaagcaattt caattttatc
     4621 gaatttttta taaaacgctt caacaataga tgccattgtg tcatcggctt cggcgccgga
     4681 gacaataagc ggcgtaaagc ctgcttcgtg tgcaatcgtt tggatatcgt cgatttggcg
     4741 gcgtagcgca tcgggcatcg gtggacggtt ggctttatag tctttgaaca tttcatagcg
     4801 cggattttct tcgcggccag ggtcgaagca cagtacaaag tgcgatggct ttctgtctat
     4861 gagcaaccgc gcgagcatgc gaaaaaaacc gaacgtggtt tcggtggcgt tgccgttttc
     4921 gtcggttaaa ttctgtcctg caaacgcgaa atgcgcgcgg tacgccaacg catggccgtc
     4981 gaccaaaacc aacgtttctt tttgtttttt tgttggtttc gtttttgtac cggacatagc
     5041 gtacgcttgc tttagaaagg caggccgtca acgagatgtg gattgaatag agttgccgcc
     5101 gactctcaca ccttaaacgt atcgtatgtt acccgattac tatcaatttt cacttcctac
     5161 caaagtgcgt tacggcattg gcttggcgtc gcgcttgggc gaggagctgg cggattttcc
     5221 cggctcacgc gcacttcttg tcaccgataa aattatcgtg agcgcaggct tagcgcaaaa
     5281 agtcattgat gggcttaaag gttcaaaaat caccatcgcc gccacgtacg acaacgtgcc
     5341 acccaacagc gaaattaaag tcgtcaccga agcggcagag ctgggcgtca aaaataaatg
     5401 cgacatggtg attgcgctgg gcggcggttc ggtgatcgac tcggcgaaag tcatcaacct
     5461 cctcatggtt aaaggcggcc ctttgcaaaa ccacatgggc gcacaattat tgaaagaacg
     5521 ccttctgccc acgattgcta tccccactac ctctggtacc ggttctgaag ccacccagtt
     5581 tgccgtcatc tctgacgatg caaactcggt aaaactccct ttcgcggaag atttctttat
     5641 gcccgacgtc gcgatcctcg accctgagat gacccaatcc atgccggggc gcgtcaccgc
     5701 agcaacgggg atggatgcgc tcacgcacgc gattgaaagc tatgtgggat tgaataacaa
     5761 tcctgtttca gatgctcttt cgctttatgc aatcgatctc attgtgaaaa atattttgca
     5821 ggcggttgca gtccccaacg acctcaacgc gcgtggcgcg atgctcactg cgtcgttctt
     5881 agcgggagtg gcgtttaacc atggcggagt ggggattgtc cacgcgatta gccatgcgtt
     5941 aggcggcgtg taccacatcc cgcacgggct tgcgaacagt atcattcttc cctttagtat
     6001 ggcgcacaac atggaatcga gcgcggcacg ctacgcgcgt atcgctgaaa ttttaggcga
     6061 cagtggattt tatcccgtgc aagagctcaa caaagctgtc gcgcggtttg acaactttgc
     6121 gctcaatcaa gcggtgacgc agctttcgtt ggtggacgat tggctcatca aacgccgcgc
     6181 cgaatcgctc gcggataagc tctccgcgcn nnnnnnnnnn nnnnnnnaga taatttagcc
     6241 aaaatcgacc agcttgtgca aaccgcgctc gacgatgcgt caatgctcaa caaccccgaa
     6301 agcgcaaccg gcgaagatat gcgtgcgatc attcaaaaag catactcgca aaatttgaaa
     6361 ccgattgcgg tttcaaaatc agagctcaaa gccgcgcgca cccaaagcgg tggcagaaag
     6421 ctcaaaggca ttttcaaaga cactgccgca ctctacgata ttttgggtac gtactttttg
     6481 aaagtacaag ccgaccccaa gctcttcgca ccgctcaaaa attcaaaact caaaatccgc
     6541 ttcaactata cagctcccga cggcgccatc gcgctcgacg gctcgaccga cgataaagca
     6601 aaacaaattt taaccgccga cgctgcacgc gcatttatcc ccgaagtgga gtttacgctt
     6661 tcatcggata tgtcgcacta cttttggcac ggtcaggtca acttgatgca agctctagcg
     6721 cgcaaagaag taatccccaa gggcaaccta ccccgcgcga tgcgtctcct cccacttttg
     6781 gaaccgctct acgaactcta cccacaatac ctgcgcgaaa gagacttagg caaattagtg
     6841 ctatgacccc tcaccccgcc gcaagagcgt cgaccctctc ccctaaggag agggttggga
     6901 taatagttcc ccctcgccct gagggagagg gggttagagg gtgagggaaa aactgtggca
     6961 cgggcgcttt gaaagcgctc cctctaaaat taccgaggaa atatccgaat cgatttcgta
     7021 cgatcatgcc ttgtacaaag tcgacattcg cgcctcgaaa gcacacgcac ggatactcat
     7081 gcaaatggga gttctcaccc ccagtgaatt tcaacaaatt gaaaagggat tagccacagt
     7141 agaagaagaa attgctgcgg gtaaaatgat tttctccccc gcgttggaag atattcacat
     7201 gcacgtcgaa tcccgcctga ctcaaattat aggcgaggtg ggaaaaaaac tccataccgg
     7261 ccgttcgcgt aacgatcaag ttgctgtcga tacgcatctt tttgtgcgcg cagcgcttga
     7321 cgaacataaa aaagatttac ggaaactcct tgtgacgctg cactcgcttg cagaaaaaca
     7381 ctttggcaaa atttggattg gctatactca cttacaaatt gcacaaccgg cgctcctttc
     7441 gcattatctt ttatcctatt tttggcagtt tgcccgtgat acagagcttc ttacttttgt
     7501 agaaaactcg gtacgctttt ccccgttggg ttcggcggcg ttgggcggcg tgaattaccc
     7561 catcgaccgc gcacaaaccg caaaagagtt gggcttcgac gcaccgtacc ccaatagcat
     7621 ggacgcggtc tccaaccgcg actaccaact gaattaccat tttggggcag tacgactttt
     7681 cttgcacatc tcgcgttttt gtgaagacgt cattatctac aattcgaccg agttcggcta
     7741 cgtgacgctg ggcgatgccg tcaccaccgg ttcgtcgatt atgccgcaga aaaaaaatcc
     7801 cgacatcgcc gaacttttgc gtggcaaaac aggacgcgtg aacggcaacc tacaagcgct
     7861 catcaccaat ctcaaaggtc tgccgatgac gtacaaccgc gacttgcaag aagacaaagt
     7921 atatcttttc gattcgatta accaggtgcg ccaaggactt ttaggtttaa ccgaaattct
     7981 caaccacatt caattcaacg aacaccgcgt tttagaaaat ttatcgcggg gctttgcact
     8041 cgccaccgat gtggcagatt ttctcgtgcg cgaaaagaac gtgcctttcc gcgacgcaca
     8101 cgcggtcgca ggggctgccg ttaaatttgc cgaaacgcac gacacccgtc ttgaaaattt
     8161 gacagccgcc gattggcaaa agattgcggg ttatacgctc acgttccccc aagatttttt
     8221 caccccgctt gcatcggtag cgcgccgtca aggctacggt tcgaccaacc ctgacgcggt
     8281 gaaaatgcaa ctggatgaag cgaaggcgtt cttagaagtg tattaatcat aatttgtatt
     8341 ttcaccctgt tacagcgtat agattgtcgt ccgtgcttca atttttgctt acgccgctgc
     8401 aaaatcacat ttcgtggttc cgcattttta attatacgac tttccgtttg gtcgcggcgg
     8461 caatcacggc ccttcttcta acgtattttt tagggccgcg ttttatccgt tttttacaaa
     8521 aactgaaatt cggcgagtcg gtgcgctccg atggtcctga aagccatcaa agcaaagcgg
     8581 gaactcccac gatgggaggt ctcatgatca tggcaagcat gagtttttca gtgctcttat
     8641 gggggaattt agcaaacctt tatgtaatgc tcctttgggt gtgcacattg gcgctgagcg
     8701 ccgtggggtt tatcgacgac tattccaaat cggtaaaaaa aattcctggc ggcatgcgcg
     8761 cgcgcactaa attttttt
//
LOCUS       DAOMPD010000039         8746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3449_length_8746_cov_2.593603, whole genome shotgun sequence.
ACCESSION   DAOMPD010000039 DAOMPD010000000
VERSION     DAOMPD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3449_length_8746_cov_2.593603"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..616
                     /locus_tag="PLY93_02310"
     CDS             104..616
                     /locus_tag="PLY93_02310"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRP68344.1"
                     /translation="MHWYTLYTAPRAEKKLYAQLKKLSIEAYLPLIKQRNKWSDRYKW
                     VEEPAFPSYIFVRLDGGNKEDFLKILRLPHARRFIILEGQVSIIPDADIELLRLSIDQ
                     YATSLKIGGLDDILVGEKVLITEGVFLGREAIVDAKEGKATVSVVFPTINQSLRVSIP
                     FSYVSRQKVQ"
     gene            complement(610..1740)
                     /locus_tag="PLY93_02315"
     CDS             complement(610..1740)
                     /locus_tag="PLY93_02315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP68345.1"
                     /translation="MDNLEWRRITSLNGIDSKAWNLLVEEDNPFMRYEFLSALEETAC
                     IGKNTTWQVNAFAAFQNDTLCAVAPAFVRYDSYGEYIFDWAWANAYHNAGLSYYPKLT
                     VAAPFTPATGRRILTGSHNLVSIASAVHALKNFSKNTVSGIHFLCTTRAEQRALSQLG
                     FAPRVTHQYHWHNRQYKSFDDYLSHLHSHRRKEIKRERKKCSEHNLRIETFAGEAIRE
                     EHIRAMYVFYCNTYARKWGSPYLNERTFFLLWKNMTQSLVLNLAHDGKSWVAGALAFY
                     TPNKIFGRYWGAVNNYPYLHFELCLYRFIEFAIRKNIATFDAGAQGEHKFQRGFMPTP
                     IYSSHLLFHTQAFDMISAFLSQERHETIQLLKASRQSSAFRH"
     gene            complement(1733..2521)
                     /gene="ygiD"
                     /locus_tag="PLY93_02320"
     CDS             complement(1733..2521)
                     /gene="ygiD"
                     /locus_tag="PLY93_02320"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRP68346.1"
                     /translation="MAPRQPVFFIGHGSPMNAIEENTFTAAWKKIGKNLHVPQAVVCV
                     SAHWVTRGTEITAHPQPKTIHDFGGFPPALYEVEYPAPGSFEIAQLVQETLKPQINAR
                     LTDAWGLDHGAWSILMHIFPDAKIPVLQLSIDATLAPQAYFDMGTRLKKLRAENILFI
                     GSGNIVHNLRLVDWTRFAEKDYALPWARKADRRIVELIRSRNFLPLIQFQNLGEEARL
                     AINSAEHYVPLLYVLGLSDEDDTLSFFNEEAVAGALTMTSVRFG"
     gene            complement(2525..2797)
                     /locus_tag="PLY93_02325"
     CDS             complement(2525..2797)
                     /locus_tag="PLY93_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68347.1"
                     /translation="MKKNNCAFTNEFILRLESEDWNSQLVSRIQETKRNRAKRRIQWS
                     ALCTALLAAALAVTTLFTDDVATDNNFYTSIEEVSGGLQMQVSFIE"
     gene            complement(2820..3338)
                     /locus_tag="PLY93_02330"
     CDS             complement(2820..3338)
                     /locus_tag="PLY93_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP68348.1"
                     /translation="MQNSSNTNIDFSKIIAETKKTVLAAIRRYLDMKYAHAIDDVAQE
                     VYIRAYRALNDGKLKESSKLRSYLYTIAKNESLRMNARLTREEIKAERFIEEERERLS
                     ALPEIENPLGEMTNRIIEELRHIPKHYANVVELSLLGFTAKEIVERLDIKPGTVKSRL
                     SRGLALLRSRVA"
     gene            complement(3322..3786)
                     /locus_tag="PLY93_02335"
     CDS             complement(3322..3786)
                     /locus_tag="PLY93_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRP68349.1"
                     /translation="MAQEIVDKSTLTRIGWTFLFFALASFMVYCKPWKHKSPAERAEA
                     MVNRISKELSLNPSQLELLNTLKEKTLTQQRLDKAEHEKQLKDFSTLFLSPTIDKAKL
                     AQLKKQHDSLRTRNESLFLENVVLMHNALTPEQRIKAMDVMMNYGKKNAEFK"
     gene            complement(3836..>4924)
                     /locus_tag="PLY93_02340"
     CDS             complement(3836..>4924)
                     /locus_tag="PLY93_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP68350.1"
                     /translation="QTIKSAGTTSKRTVFHRVLLEAGAPEPTKGFPLEGTFTSLIRDE
                     KLRLAYSESEKYTASFFMKPTGEFIVQQGQYMPNIRSVPTFPENKDPQLVGDANLEIG
                     ATWESAGEEVMKFSKLISVPFNVRYEYRGIENIKSEEGEKSCHKFISNYELSYGNDET
                     GKPRVFGYVTAVWFWDAAQGIPYYATEEYNVILVNDEGVANEFKINSRSYYRKFRKRV
                     EENKIELVTNLKEKLTEQNLPLDVRITDNGVAISLPDVFFETDSVELNRAAKKTINQI
                     AENLKQIDFPHIRVRGHTDSVGDEAYNQELSERRAEQVADFLIDGGELNADKISYDGR
                     GSKDPIADNETIEGRAKNRRVEIILLDK"
     assembly_gap    4925..4934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4935..5196)
                     /locus_tag="PLY93_02345"
     CDS             complement(<4935..5196)
                     /locus_tag="PLY93_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68351.1"
                     /translation="MVNLPKFAVINYVQSTLMRKLISAMRFLQKMNSKAMLLFALLLS
                     IDIVEAAPRLRQSKKTAPNVFAEAQGKLLRWQFAQGEVLELKK"
     gene            complement(5222..6247)
                     /locus_tag="PLY93_02350"
     CDS             complement(5222..6247)
                     /locus_tag="PLY93_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68352.1"
                     /translation="MVHMKFKHIKAPARTLVVSTLDVCYNAFTVYFLKLWCERFATKP
                     DFNVDSILTHAYSVYQEIPTRYSTFLKFILTQKYKTVIFLNPNAREDRALRWATRTAL
                     IKNRVGFNPLKGVNPLNLSLPFNTENHHYVHQLKIFFEYITGEKVHHWENPSLPSIPE
                     NTNPVYSAAVLSLDAHATASKRLAGEFAKLINFVTRTTRLTLFISGSQEATQNLSRTL
                     SVPMTEKALTNTLLTLNPSEEEKLLAVRNALWVTGTDICTLNMAGLAQTPSLSIFGPL
                     NERVWQPFSTRARILAGEFDCRPCTHYPGKVECKNPLAWQCLSGVTAELILATLNGML
                     TKHASKP"
     gene            complement(6228..7271)
                     /locus_tag="PLY93_02355"
     CDS             complement(6228..7271)
                     /locus_tag="PLY93_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP68353.1"
                     /translation="MLCRISSNGEIPLGFGIYPTPIEKERQAVFDAVKRRFNGIVILE
                     EKADIPRIAHRLGTSWEHAASLTRRRRLQLLAKRFNAQIATGHNYSDYEETLELRRRR
                     GIPEAGIPPLRLVDSATQFMRPLYRMRRDTVRQKVTALGLPYFDDPENTNTDFERNRI
                     RYECNQKARRVRKPANRGRPSVSLPTVIPRRELRYNAARFHARTNVEKARLVFQAFRT
                     LAIVHPFTRNDFERARRLPFALPPFFAHEEIIHQKKYIVFRRGLGVFMPITKTPEKHC
                     VRGDSVTRSTSIAMPYGKKSVAKILSERKLSPRERRLTYVYLQSDSPHTCTKIHFSTG
                     EILSAWKDGSHEI"
     gene            complement(7319..7948)
                     /gene="pth"
                     /locus_tag="PLY93_02360"
     CDS             complement(7319..7948)
                     /gene="pth"
                     /locus_tag="PLY93_02360"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRP68354.1"
                     /translation="MALSDLKLVIGLGNPGDRYMNNRKNIGFKVVDVLANNTGIQIKT
                     KKKKSLLGQGDFEGHDIVLLKPQTFMNLSGEAVLYIASFLRVKVENTIVVHDDITMKY
                     GEVVVAQGPSEIPNVGVESIARGLKSDKFVRIRMGVGPVKIRGKEYPNPKENEINAFM
                     LSDFSLEENMKIIEVINDAEAALRSILTQDIKDVLQRFKLGAKFTRSRP"
     assembly_gap    8086..8095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8096..8604)
                     /locus_tag="PLY93_02365"
     CDS             complement(<8096..8604)
                     /locus_tag="PLY93_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRP68355.1"
                     /translation="MEKATLKATKRDGKIGGKNLLRSGIIPAVIYNHGKSESIQVFEK
                     DTRKLFAQGISESLLINLDIEGKVEQVFVKQYQAHPVNDVLQHLDFYRVSQDEKVRTR
                     IAVRLEGKAQGVKDGGILEVFLTEVPYETYPKHLTEALVIDISDLQIGASLHVANVQL
                     PPDSKILMDP"
BASE COUNT         2137 a         1898 c         2182 g         2509 t
ORIGIN      
        1 ccataaatcc gtaaatagta cttttcgcaa gacgcagttt ttttattttg tattatagaa
       61 tcctctgttc gacgctttgc gttacgggga tttgaaatta tcgatgcact ggtatacact
      121 ttacacagcc cctcgcgctg aaaaaaaact ctatgctcag ctcaaaaagc tttcgattga
      181 agcatacttg ccccttataa aacagcggaa taaatggtca gaccgctaca agtgggttga
      241 agaacccgct tttccttctt atatattcgt gaggctcgat ggtggcaaca aagaagactt
      301 tctcaaaatt ttgcgcctac cgcatgcgcg tcgttttatt atacttgagg ggcaggtatc
      361 gattataccc gacgcggata tagaactctt gcgcctgagt atcgatcaat acgcaacatc
      421 gctgaaaatc ggaggtcttg acgatattct tgtcggcgaa aaagtgttga tcaccgaagg
      481 agtattttta gggcgcgagg ctatcgttga tgccaaagaa ggcaaagcaa ccgtgagtgt
      541 tgtcttcccc acgataaacc agtcgttgcg cgtatcgatc cccttcagct acgtcagtcg
      601 acagaaagtt caatgacgga aagctgaaga ctggcgtgaa gctttgagca actggatggt
      661 ttcatgccgc tcttggctta aaaaagctga aatcatatcg aaagcttgcg tgtggaatag
      721 aagatgactg ctataaatgg gcgtcggcat gaatccacgt tgaaatttgt gctctccttg
      781 tgcgccagca tcgaaagttg caatattttt cctgatagca aattcaataa aacggtaaag
      841 acatagctca aaatggagat aagggtaatt gttgactgct ccccagtagc gcccaaaaat
      901 tttgtttggc gtgtaaaagg caagcgcacc ggcaacccac gattttccat cgtgcgctaa
      961 gttgagtaca agagattgcg tcatgttttt ccataataag aaaaaggttc gttcatttaa
     1021 gtaaggactc ccccatttgc gtgcgtacgt gttgcaataa aatacataca tcgcgcggat
     1081 gtgttcttct cgaatcgctt cccccgcaaa cgtttcaata cgcaaattgt gttcgctaca
     1141 ttttttgcgt tcacgcttaa tttctttgcg acggtgtgaa tgtaagtggg agagataatc
     1201 gtcgaaactt ttgtattgtc gattatgcca atggtattgg tgcgtgacgc gcggcgcaaa
     1261 acctaactgg gagagagccc gttgttcggc gcgggttgtg cataaaaagt ggattcctga
     1321 aacggtgttc tttgaaaaat ttttaagcgc gtggacggca gaggctatcg aaactaaatt
     1381 atgtgaaccg gtaaggatgc gacgtccagt tgcaggggtg aagggtgcgg cgacggttag
     1441 cttggggtaa taggaaagtc ccgcgttgtg gtatgcattc gcccacgccc aatcgaaaat
     1501 atattcgcca tacgaatcgt agcgcacaaa agcgggagcg accgcgcaca gggtatcgtt
     1561 ttgaaacgcg gcaaaagcgt tcacttgcca tgttgtattt ttaccgatac aggccgtctc
     1621 ttctaacgcc gacaaaaatt catagcgcat gaacggatta tcctcttcga caagtaagtt
     1681 ccatgcttta ctgtcgatgc cattgagcga cgtaatacgt cgccattcaa ggttatccaa
     1741 aacgaaccga tgtcatggtg agtgcaccgg caacggcctc ttcgttgaaa aatgaaaggg
     1801 tgtcgtcttc gtcgcttaat cccaaaacat aaagcaaagg gacgtaatgc tccgcgctgt
     1861 tgattgcaag acgcgcttct tctcctaaat tttggaattg gataagcggc aaaaaattac
     1921 gcgagcgtat caattcgaca atgcgacggt ctgctttgcg cgcccacggc aacgcataat
     1981 ctttttcggc aaaacgcgtc caatcgacga ggcgtaaatt gtggacgata ttaccgctgc
     2041 cgatgaataa aatattttct gcgcgtaatt tttttagtcg agttcccata tcaaagtatg
     2101 cttgtggagc gagggttgcg tcgatagata attgcaacac aggaattttt gcatcgggga
     2161 atatatgcat gagaatactc catgcgccgt ggtcgagtcc ccaagcgtcg gtgagccgtg
     2221 cattgatttg gggcttaagc gtttcttgaa cgagttgcgc aatctcgaaa gagcccggcg
     2281 ccggatattc aacctcgtaa agcgcggggg gaaagccgcc aaagtcatgg atggttttag
     2341 gttgtggatg ggcggtgatt tctgtgccgc gcgttaccca gtgtgcggaa acacaaacaa
     2401 ccgcttgagg tacgtgtaag ttcttaccga tttttttcca cgctgccgtg aatgtatttt
     2461 cctcgatagc gttcatcggc gaaccatggc cgataaaaaa tacgggttgt cgcggcgcca
     2521 aagtttactc gataaaagaa acttgcattt gtagaccgcc ggagacttct tcaatcgaag
     2581 tgtagaagtt attgtcggta gcgacatcgt cggtaaagag cgtggtcact gcaagcgccg
     2641 cagccaagag ggctgtgcat agagcactcc attggatacg tctttttgca cggttgcgtt
     2701 ttgtctcttg aatgcggctt acgagttgag agttccagtc ttcgctctct aagcggagga
     2761 tgaactcgtt ggtgaaagca caattattct ttttcatttt ttatttccac tatataactc
     2821 tacgcaactc tgcttcttaa aagtgcgagt ccccgcgata agcgtgattt cacagtgccg
     2881 ggtttaatgt cgagccgctc gacaatttct tttgctgtaa agcctaaaag cgaaagttca
     2941 acgacgtttg cgtagtgttt ggggatatgg cgcagctctt cgataatacg attggtcatt
     3001 tcaccgagag gattttcaat ttcaggcagt gcgcttaaac gttcgcgttc ttcttctata
     3061 aaccgctctg cttttatttc ttctcgggtt aaacgagcat tcatgcgcaa cgattcgttt
     3121 ttagcgatag tgtaaaggta tgagcgcaat ttcgacgatt ctttgagctt accgtcattt
     3181 aatgcgcggt aggctcggat atacacttct tgtgcaacgt cgtcgatagc gtgagcgtat
     3241 ttcatatcta aataacgacg gattgccgcc agaacagttt ttttagtctc ggcaataatt
     3301 tttgaaaagt ctatattggt gttacttgaa ttctgcattt ttctttccat aattcatcat
     3361 tacgtccatt gctttgatgc gttgttcggg cgtcaaggca ttgtgcatga ggacgacatt
     3421 ttctaaaaac aacgattcat tacgggtacg tagtgaatcg tgctgctttt taagttgagc
     3481 gagctttgct ttatcgatag tgggcgataa aaatagcgtt gaaaaatctt tgagttgttt
     3541 ttcatgttcg gctttgtcaa gacgctgctg ggtgagagtt ttttccttta atgtgtttaa
     3601 cagttcaagc tgcgaaggat tgagcgaaag ttctttcgat atgcggttta ccatcgcttc
     3661 ggcacgctcg gcaggtgatt tgtgtttcca gggcttacaa taaaccataa acgaagcaag
     3721 agcaaaaaaa agaaacgtcc atccaattcg agttaaagta ctcttatcta cgatttcttg
     3781 cgccatactg tgtagacctt gatagttcgg ttttggtttc cacattttgt acgacttact
     3841 tatctaataa aatgatttct acacggcggt ttttggcgcg tccttcgata gtttcgttat
     3901 cggcaatggg atcttttgag ccgcgtccat cgtaactaat tttgtcggcg tttaattcgc
     3961 cgccatctat taggaagtcg gcaacttgtt ctgcgcggcg ttctgaaagt tcttggttgt
     4021 atgcttcgtc gccgacagaa tcggtatgac cacgcactcg tatgtgaggg aaatcaattt
     4081 gttttagatt ttcggcgatt tggtttatcg tcttttttgc cgcacggttt agttcgacac
     4141 tgtcggtttc aaaaaaaaca tcaggtaaag aaatggcaac accgttgtcg gtgatgcgta
     4201 catcgagcgg gaggttctgt tcggtgagct tttctttaag attagtgact aattcaattt
     4261 tgttctcttc gacgcgttta cgaaacttgc gataatagct gcggctattg attttgaatt
     4321 cattcgcaac accttcgtcg ttgacgagaa taacgttata ttcttcggtg gcgtaatagg
     4381 ggatgccttg tgcggcatcc caaaaccaaa cggcggtgac gtaaccaaaa acgcgcggct
     4441 tacccgtttc gtcgttgccg taagaaagct cgtagttaga gatgaacttg tgacaactct
     4501 tttcgccttc ttcggattta atgttctcaa tgccgcggta ttcgtaacga acattgaacg
     4561 ggacagagat gagttttgaa aatttcatca cctcttcgcc ggcactttcc cacgttgcgc
     4621 cgatttctaa gttggcgtcc cccacaagtt gtggatcttt attttcgggg aaagtgggta
     4681 ccgatcggat gttcggcatg tactggcctt gttggacaat aaactcgccg gtgggtttca
     4741 taaaaaaact ggcagtatat ttttccgatt cgctatacgc caagcgcaat ttttcgtcgc
     4801 ggatgaggct tgtaaaagtt ccctcaagtg gaaagccttt tgttggctca ggcgcaccgg
     4861 cttccaaaag aacgcggtgg aaaacagtac gctttgaggt tgttcccgct gatttgatcg
     4921 tttgnnnnnn nnnnattttt taagctcgag cacttcgcct tgtgcaaatt gccagcgtaa
     4981 aagtttgcct tgcgcttcgg caaaaacatt gggggcagtt tttttgcttt ggcgcaagcg
     5041 gggcgcagct tctactatgt caatgcttaa taaaagggcg aagagaagca tcgcttttga
     5101 attcatcttt tgtaaaaaac gcattgcact tatgagtttg cgcatcaacg tggattgtac
     5161 atagttaata acggcaaatt taggaagatt gaccacaaat tgtatttgtg cggtgcgtaa
     5221 atcagggttt tgaggcgtgc tttgtaagca taccgttgag cgttgctaaa ataagctcgg
     5281 cggtcactcc cgaaaggcat tgccacgcaa gaggattctt acactcgacc ttgccaggat
     5341 agtgggtgca agggcggcaa tcaaattcac cggcgaggat acgcgcccgt gtcgaaaacg
     5401 gctgccacac gcgttcgttg agggggccaa aaatactcag cgagggggtt tgtgcgaggc
     5461 ctgccatgtt gagagtgcaa atgtccgtgc ccgtgaccca gagcgcgtta cgtacggcga
     5521 gcaacttttc ttcttcgcta gggttgagcg tcaacagcgt gtttgtgagc gccttttctg
     5581 tcatggggac gctcagcgta cgggataagt tttgagtggc ttcttgcgaa ccgctgatga
     5641 ataacgttaa acgcgttgtg cgcgttacaa aattgatgag ctttgcaaat tcaccagcaa
     5701 ggcgcttgct cgcggtagcg tgcgcgtcga gcgataacac cgctgcgcta taaaccgggt
     5761 tggtattttc gggtatcgaa ggcaaagagg gattttccca atggtgcact ttttcaccgg
     5821 tgatgtactc aaagaaaatt ttgagctggt gtacatagtg atggttttct gtgttgaaag
     5881 gcagcgataa atttaaggga ttaactcctt tgagcggatt aaagccgaca cgatttttga
     5941 taagcgctgt gcgtgttgcc catcgtaacg ccctgtcttc gcgcgcgttg ggatttaaga
     6001 aaatcacagt tttatatttt tgagtaagga taaacttcaa gaatgtacta tacctcgtcg
     6061 gtatttcttg gtatactgag taggcgtggg tgaggatcga atcgacgttg aaatcgggtt
     6121 tggtggcaaa acgttcgcac caaagcttca agaaataaac ggtgaaagca ttatagcaca
     6181 catcgagcgt tgaaacgaca agagttctcg cgggggcttt gatgtgctta aatttcatgt
     6241 gaaccatctt tccacgccga taagatctcc cctgttgaga aatgaatttt tgtgcaggtg
     6301 tgcggtgaat ccgattgcag atacacgtag gtcaatcgtc gttcgcgagg ggagagtttg
     6361 cgttcgctca agattttagc cactgatttt tttccatagg gcatggcaat cgatgttgaa
     6421 cgggtgacgc tgtctccccg cacacaatgt ttttctggcg ttttagttat aggcatgaaa
     6481 acacccaaac cacggcggaa gacaatatat tttttttgat ggataatctc ttcgtgcgca
     6541 aagaagggtg gtaaggcaaa gggaagccgg cgcgcccgtt cgaaatcgtt gcgcgtaaac
     6601 gggtgtacaa tagctaaggt acgaaacgcc tgaaaaacaa gacgcgcttt ttcaacgttt
     6661 gttcttgcat ggaagcgcgc cgcattgtaa cgcagctcgc gccgtgggat gaccgtgggg
     6721 aggcttaccg atggtctgcc acggttggca ggttttcgta cgcgccttgc cttttggtta
     6781 cattcgtagc ggattcgatt acgttcaaaa tcggtgttgg tgttttcagg atcgtcgaaa
     6841 tacggtaaac cgagcgcagt cactttctgc cgcaccgtgt cgcggcgcat gcggtaaaga
     6901 ggccgcataa attgagtggc gctatcgacg aggcgtaaag ggggaattcc cgcctcgggg
     6961 atgccgcgcc gtctacgcaa ctcgagcgtt tcttcgtagt cggaataatt gtgccctgtg
     7021 gcaatttgtg cgttgaatcg tttggccaaa agctgcaacc ggcgccgcct tgtgagcgat
     7081 gccgcgtgtt cccaactggt gcccaggcgg tgcgcgatac ggggtatatc cgccttttct
     7141 tcgaggataa ctatgccatt gaagcggcgc ttaaccgcat caaaaacggc ctggcgttct
     7201 ttttcaatcg gtgtggggta aataccaaag ccaagcggga tttccccgtt ggacgatata
     7261 cgacagagca agcacgctat cggcgccgcc cgaaaacgcg acgatgtgca accgtttttt
     7321 agggacggct tcgtgtaaac tttgcaccta acttaaaacg ctgtaacacg tctttaatgt
     7381 cttgcgttaa aatcgaacgc aaagcggcct cggcgtcgtt gatgacttcg ataattttca
     7441 tgttctcttc gaggctaaaa tcggagagca tgaatgcgtt aatttcattt tctttggggt
     7501 tgggatattc tttgccacgg attttaacgg gaccaacgcc catgcggatg cgcacaaact
     7561 tgtcggattt aagaccgcgt gcaatcgact ccacaccgac gttgggtatt tcagaagggc
     7621 cttgggctac cactacttcg ccgtatttca tggtgatgtc gtcgtgaacc acgattgtgt
     7681 tttcgacttt cacgcgtaag aacgaagcga tgtaaagcac cgcttccccc gacaaattca
     7741 taaatgtttg cggcttcaag agcacaatgt catgcccctc gaaatcgcct tggcctaata
     7801 gcgatttttt ctttttggtc ttaatttgaa taccggtatt gtttgctaaa acatcgacga
     7861 ctttgaaacc gatgtttttg cggttattca tatagcgatc gcctggatta cccaagccaa
     7921 ttactagttt aagatctgaa agagccatta cgctgttaga agttacctac aaatttattt
     7981 ttttgcctct gccttggggg cttctgcttt agccgcagct ggcgccgctg cagccggcgc
     8041 cgcttcggtc gcttcctctt ttgcttggtg cgtgatattg caaatnnnnn nnnnnggatc
     8101 catcagaatt tttgaatcgg ggggtagctg tacgtttgca acgtggagcg atgcaccgat
     8161 ttgtaaatcc gagatgtcga tcacaagcgc ttcggtcaaa tgcttagggt atgtttcgta
     8221 tggcacctcg gtgaggaaca cttcgagaat accgccgtct ttaacgcctt gcgctttacc
     8281 ttcgaggcgg acggcgatac gtgtgcgcac tttttcgtct tgcgacacac ggtaaaaatc
     8341 gaggtgttgc aacacgtcgt tgacagggtg cgcttggtat tgtttcacaa aaacttgttc
     8401 gactttacct tcgatgtcga gattgatgag cagcgattct gaaatacctt gtgcaaaaag
     8461 cttgcgggtg tctttttcaa aaacttgaat gctttcgctt ttaccgtggt tataaatcac
     8521 ggcgggtatg atgcccgaac gtaacaaatt tttaccgcct atttttccgt ctcgctttgt
     8581 cgctttaagt gttgcctttt ccattttatt ctcccttatt taattacaag aacattgaac
     8641 tataaaaata acgaactcac cgatttctct tcgtggatgc gcttaatcgc catgccaaaa
     8701 agcgccccta ccgataaaat gcgcatgcgc gggaaacgct tttctt
//
LOCUS       DAOMPD010000040         8731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3455_length_8731_cov_3.412633, whole genome shotgun sequence.
ACCESSION   DAOMPD010000040 DAOMPD010000000
VERSION     DAOMPD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3455_length_8731_cov_3.412633"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..437)
                     /gene="nuoK"
                     /locus_tag="PLY93_02370"
     CDS             complement(123..437)
                     /gene="nuoK"
                     /locus_tag="PLY93_02370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRP68356.1"
                     /translation="MTEFLSVQAIVVVAFILFAIGIVGVVARRNLIVILMSVEIMLNA
                     VNLIFIAFSRSFNDTIGQVMVLFVMAVAAAETAIGLALIIALFRNARSVETTAMQNLK
                     DL"
     gene            complement(443..1000)
                     /locus_tag="PLY93_02375"
     CDS             complement(443..1000)
                     /locus_tag="PLY93_02375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRP68357.1"
                     /translation="MEEISLRQTLFYCFAFVLIGSASGVVFHRNPIKSALFLITFFVS
                     LAATYALIGAEVLATFQVLVYVGAIMVLFLFVIMLIAVREENFESPGSHLGRTLIISG
                     LALAFLIQVFMLLQVRIGRVDLNPNAQPATAVKAGQNITDTTQVIGVSLFTDYLVAFE
                     LVSLLLLVAVVGAIMIAKHNRRGLE"
     gene            1145..2857
                     /locus_tag="PLY93_02380"
     CDS             1145..2857
                     /locus_tag="PLY93_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRP68358.1"
                     /translation="MLKYTLRTLSVIGALVFAIHCSSAKAKTTNESYAENFSFTKNDT
                     NNCGTGAKICLTSKVISRELVDAVSNNVLLDSRAVTAVEYQDCVDAKQCESKGRAKDA
                     SQPMTNLSFEHANRYCAWAGGRLPTIAELKAGMQNENFVKSDASEWSNDWAPVTGDKC
                     IVNKKNICEPRNPMGIASGIYPYPTLKLKQTLNTSTGTVASVETTSTKNHIFRCASDS
                     QPPTKAPPWMEANPPVALGDPDAPSSSELKKLHNLEATDTLNKPICKEKYTSPAHCKD
                     PMTYFTPNESQNYVFAPYIRNLGGGYAGVAADANYSYIALAKSRFVWLFDFDVNINAL
                     HRIIRAFILESENVPIFLTRFEKKNKEASLAIIKKYYADRDDVDFIQQVFRNNHEALG
                     KHYKKNATPDKRHPDFGWLAVAENYRYIRLLYTQDRIAIVPGDMLKDKTMQSIGKAAK
                     SIGIPIRVYYPSNAEEFWDYSTSNSNYKRNILSLPFDEASITFRTVHEYPWHPKWRKG
                     MQGFWHYVVHGAYNYQKKLMLPHFDYMDYFKMFRVVSSTRADFSTIEIPAKLPKNILE
                     QGNP"
     gene            2864..3685
                     /locus_tag="PLY93_02385"
     CDS             2864..3685
                     /locus_tag="PLY93_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP68359.1"
                     /translation="MRQLAQRVQRGNFFAVFFIGGILGFACHAAKKNQSDAPKEIYAL
                     KYGESLYSARLVNTRQTTSHVKLNWLAYLIIHHDGTRTLVDTGFSDKNLLKRFAIQKF
                     KSVPAILALLHIDSSQIQRIILTHTHFDHALDLDLFSNAKVFLHKKEFSAPQEKRVAD
                     KLKFFAMQGKVQTFQKEITHAGLTLEFVGGHTQGSIVVKTIANNNSLLFTGDECYFAE
                     SCFAGVALPSAAAFDVHANRRFIESIKPQTKILTGHEIQFSQGEWLNDYIFRFSF"
     gene            complement(3661..4782)
                     /locus_tag="PLY93_02390"
     CDS             complement(3661..4782)
                     /locus_tag="PLY93_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68360.1"
                     /translation="MSREHSNYLEYTSSGSYTNGSVEKLFNKLDKYGNIICDMLYHNI
                     EYDKGIATDFSEVLNFEKPKKHLWYADMLPALIAGCANFYRMNDRGVHKVFRILEGLY
                     PEKKDCFTVGFTQFYMMYEEMQCARMVSIDADWRILRAHYEFQKNVREKTPSDTFLTM
                     LQKINVNWLLSFDRPPRAKEVVRDISTFCMDTEYHFCKKAFENYITLDSIPPTELMLG
                     FIHDLTFTPEEKRTTSVIFMSNALDDDYTSLEHFDKLLSRTRETLHVGQKAAFVYQAG
                     DSEDIVVYELFKSDKDELNVSIRCRDNIRWSDQYVLSKRGKPFNTHFDDLLELRKLRR
                     IPRCHQGGTKDYNDPKKSKKYLDAEKKNKLKVQKEKRKI"
     gene            complement(4895..6079)
                     /locus_tag="PLY93_02395"
     CDS             complement(4895..6079)
                     /locus_tag="PLY93_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68361.1"
                     /translation="MNPTNDGLPHRKETKPVVVGVEEIYKHPITLKDKARFFDCSEYS
                     PVTRPWHSETPYFDVFNDTLNDERRFYIAEQNGYPFVVGYHDLKRVRPDTTWMVAMRG
                     ESSGFHSTAVAKGVRNLMEDLPGAALITIDEYKYKYSKTPLKDPKPLHPICHRTNYPE
                     GRIFTETPAFLNGGGVEFLKRVLPTANPLIFISYSNSTTPRGEFLTRRLLTDEKLNYR
                     QTKDPKAFLNEYILKTGFDERVYSGIKGFIDIEGNYEYNKALWDLLSYIKLEVERDPR
                     KFYYSIMRIEKVDAYPVQTTMIRALELKGVEEENGIIRFSNPTGNVIIDMVTNHYEPY
                     YSGNGGDIRKNTTVKVYPGKVKRTVSNHYMLAPLMRKRLLETTPFLKKEMQPPPETAR
                     PS"
     gene            complement(6249..7796)
                     /locus_tag="PLY93_02400"
     CDS             complement(6249..7796)
                     /locus_tag="PLY93_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68362.1"
                     /translation="MLYAVLLGILALLGVLTWLLTMRKSNAFHLAQKLMSEGNYDEAI
                     TRLTEIAENPNHYARANVYLAECHEKMDARDLARRHYRKAIDAGAFESNERVVEVFLR
                     IIELYRVDGDYEGIFETSLEILKLDPKNEQANQEVGLLALGDGQFAVAETYLAAALEN
                     SDDAQIQLAYAVASWQRGEQDNALSVMEQALESNPDNETIKLVFATMATSGSRLPQGK
                     KASLELIQSATNLSSLSLLFDLYLWQCYQAKAWREALDFLRQRNEQNKLPEEKKMEYQ
                     YILLVLYLHEEMFLEASRLLRDLDDGEQSYRDMKHLKTCIDQIDLNPHTESIKPFKQI
                     LSDTFSGLLPNDLTYTVSGFKKNRGIAFTKYFDFVAGEPVLRIEYDAVTPEKGTNLFQ
                     QMPPDEFQRFVLYIITRLEYNEPVKENSGEKDLILYSAISSKSKSIRALFAFYRLRGD
                     AHISDISLRNLQNKMQLLKADKTYIISGAQMTEGAQVVLAAESTMKQFAGDRLAEWLQ
                     DFYKARR"
     gene            7890..>8731
                     /locus_tag="PLY93_02405"
     CDS             7890..>8731
                     /locus_tag="PLY93_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRP68363.1"
                     /translation="MAMPSLLRGKSKDETQTIHSLLAHLPSYVTRSEKALVKKAYLLA
                     EDAHKFQKRLSGEPYIIHPLAVAHTLADLGLDHEVVCAGLLHDVLEDTFIKPDYMRQE
                     FGETITYLVESVTKISVLKSQSAENKQAVNIRKMIMATVRDNRVILIKLADKLHNMRT
                     LGFQPTAKGMHIAREVLEIYAPLAGRLGIYKIKSELEDLALYHLDRTAYATIKEQVAE
                     KKGQRDGRVSMVMSLLTTQLKEAKIKAKVEGRSKHFYSIYNKMQEQKKDFEDLYDLTG
                     VRVLV"
BASE COUNT         2420 a         2032 c         1970 g         2309 t
ORIGIN      
        1 ccagtactgc catttgtcgc gtccaaagcg ttcggatgca ccgtaaatcg cagcgttgac
       61 gaccgctgca atgaggggca tgagggggat agcaaacaga aaatagggga ttaactcggt
      121 tttcatagat cttttaagtt ttgcattgcc gtcgtttcaa cgctacgcgc gttacggaaa
      181 agcgcaataa tgagcgcgag cccaattgcg gtttcggcag ccgccaccgc catcacaaaa
      241 agcaccatca cttggccgat ggtgtcgttg aaactgcgcg agaacgcaat gaatattaag
      301 ttaaccgcgt tcagcatgat ctctaccgac atcaaaatga cgatgagatt gcgccgcgca
      361 acaacgccga caataccaat cgcaaacaaa ataaacgcaa cgacaacaat cgcctggact
      421 gacaaaaact ctgtcatttg tattactcca accctcgtcg gttgtgtttg gctatcatga
      481 ttgcccccac cactgcaaca agcaataata aagaaactaa ttcaaatgcc actaaataat
      541 cggtgaagag gctcacccca atcacctgcg tggtgtcggt gatgttttgg cctgctttaa
      601 ccgctgtcgc tggctgcgca ttgggattca aatcgaccct gccgatgcgt acttgtaaca
      661 acataaaaac ttgaatgagg aatgccagtg cgagtccgct aatgatgagt gtgcggccta
      721 aatggctgcc cggactttca aaattttctt cgcgcactgc gatgagcatg atgacaaaaa
      781 gaaagagcac catgatggcg ccgacgtaca cgagcacctg gaatgtcgcc agcacctctg
      841 cgccgatgag cgcatacgtt gcggcgagag aaacaaaaaa cgtaattaaa aacaaggccg
      901 actttattgg attgcggtgg aaaacgaccc ctgatgcaga gccgataagc acaaatgcaa
      961 aacaataaaa gagggtttgt ctgagactaa tttcctccac acgtgcggtg ttaataacct
     1021 gctttcggtg gaaagcattt tgtagtttag cggaataaaa ctcaagagcc tgtgagcccg
     1081 cacccggcgc aaaagcctca atacacctga attgactgca tgtcaaaaaa aatcaaacta
     1141 accgatgctt aaatataccc tgcgcacttt gtcggttata ggtgcgcttg tttttgcgat
     1201 tcactgttcg tctgcaaaag caaaaacaac caacgaaagt tatgccgaaa attttagttt
     1261 cacaaaaaac gacaccaaca attgcggcac aggcgcaaaa atttgtttaa cctcgaaagt
     1321 aatttcacgc gaactggtag acgccgtttc caataacgta cttctcgaca gccgcgccgt
     1381 taccgccgtc gaataccaag attgcgtgga cgcgaaacaa tgcgaaagca aaggccgcgc
     1441 aaaagacgca tcgcaaccca tgacaaacct ttcttttgaa cacgccaacc gttattgcgc
     1501 atgggcgggt ggccgcctac ccacgattgc cgaattaaaa gcaggtatgc aaaatgaaaa
     1561 ttttgtaaag agcgacgcct ccgaatggtc gaacgattgg gcgcccgtta ccggcgataa
     1621 atgtattgtg aacaaaaaaa atatttgcga gcctcgtaac cccatgggga ttgcgagcgg
     1681 tatttatcca taccccacgc taaaattaaa acaaacgctc aatacatcga caggcactgt
     1741 tgcatcggtg gaaacgactt ctactaaaaa tcatatattt cggtgcgcgt cggatagtca
     1801 accgcccaca aaagccccac cttggatgga ggcaaatccc cctgttgctt taggcgatcc
     1861 tgatgcgccg tcgtcgagcg agctaaaaaa actccacaac ctcgaagcaa ccgacacgct
     1921 caacaagcca atttgcaaag aaaaatacac gtcgcccgcg cattgcaaag accccatgac
     1981 gtacttcacg ccgaatgaat cacagaatta tgtctttgca ccgtacatcc gcaatttagg
     2041 cggcggctat gccggcgttg ccgccgatgc aaactattcg tatattgccc tcgcaaaaag
     2101 tcgtttcgtg tggcttttcg attttgacgt gaacatcaac gcactccacc gaattatacg
     2161 cgcattcatt ctcgaatcgg aaaacgtgcc gattttcctt acacgcttcg agaaaaaaaa
     2221 taaagaagcg tcgctagcta tcataaaaaa gtattatgca gaccgcgacg atgtggattt
     2281 catccaacaa gtgtttagga ataaccacga agcgctcggc aagcactaca aaaaaaatgc
     2341 aactcccgat aaacgccacc cggattttgg ttggctcgca gtggctgaaa attaccgcta
     2401 catccgctta ctctatacac aagatcgtat cgctattgta ccaggcgata tgctgaaaga
     2461 caaaacaatg caatcgatcg gcaaggcggc aaaatcgatc ggtataccaa tccgtgtcta
     2521 ctaccccagc aacgcagaag aattctggga ttactccacc agcaactcaa actacaagcg
     2581 caacatttta tcgctccctt tcgacgaagc ctcgattaca ttccgcacgg tgcacgagta
     2641 tccatggcac cccaaatggc gtaaagggat gcaggggttt tggcactatg ttgtccatgg
     2701 cgcatacaac taccaaaaaa aactcatgct cccacatttc gactatatgg attatttcaa
     2761 aatgtttcgc gtcgtttcat cgacacgcgc cgattttagc accattgaaa tccctgcaaa
     2821 actccccaaa aatatattag aacaaggcaa cccttaaaag cctgtgcgcc aactcgcgca
     2881 gcgtgtacaa agaggcaatt tttttgcagt cttttttatt ggtgggatac tcggctttgc
     2941 gtgccatgct gcaaaaaaga accaaagcga cgcacccaaa gaaatatatg cgcttaaata
     3001 cggagaaagt ctttactccg cgcgcttagt gaacacccgc caaacgacaa gtcatgtgaa
     3061 actaaattgg ttggcatacc tgattataca ccatgacggc acgcgtactc tcgtggatac
     3121 cggcttttcc gacaaaaatc ttttgaaacg ctttgcgatt caaaaattca aaagcgtacc
     3181 tgccatttta gcgttattac atatagatag ctctcaaatt caacggatta ttttgacgca
     3241 cacccatttt gatcacgccc tcgatctgga tttatttagc aacgccaagg tttttttgca
     3301 taaaaaagaa tttagtgcgc cccaagaaaa gcgcgttgcc gataaactaa aatttttcgc
     3361 aatgcaaggg aaagttcaaa catttcaaaa agaaataacc cacgccggat taactctcga
     3421 atttgtcggc ggccacacgc aaggctccat cgttgtgaaa acaattgcga ataacaactc
     3481 cctccttttc acgggggacg aatgctattt cgccgaaagc tgtttcgccg gtgttgcatt
     3541 gccgtctgcg gcggcgttcg atgtacacgc gaatcggcgt tttattgaga gcattaagcc
     3601 ccagacaaaa atcctcaccg gccacgaaat ccaattttcg caaggcgagt ggctcaacga
     3661 ttatattttt cgcttttctt tttgaacttt tagtttgttc tttttttctg cgtctaaata
     3721 tttttttgat tttttaggat cgttataatc ttttgtgccg ccctgatggc agcgcggaat
     3781 tctacgcaat ttacgcagct ccaataaatc gtcgaagtgc gtgttgaaag gtttgccacg
     3841 cttactgagt acatattgat ccgaccagcg tatgttgtcg cggcagcgga tggaaacgtt
     3901 gagctcgtct ttgtcgctct tgaagagttc ataaacaaca atgtcttcac tatcgcccgc
     3961 ttggtacaca aaagcggctt tttgccccac atggagagtc tcacgggtac gagagagaag
     4021 tttatcaaaa tgctccaacg aagtatagtc gtcgtctaaa gcgttactca taaaaataac
     4081 gctcgtcgtt cttttttctt cgggagtaaa agttaaatcg tggataaaac ccaacatgag
     4141 ttctgtgggc ggtatggagt cgagagtaat atagttctcg aacgcttttt tgcagaaatg
     4201 gtactcggta tccatgcaga aggtcgaaat gtctcgcacc acttcttttg ctctgggagg
     4261 gcgatcgaaa ctcaacaacc agttgacgtt gattttttgt aacattgtga gaaaggtatc
     4321 gctgggtgtt ttttcacgca catttttttg aaactcgtaa tgcgcacgga gaatacgcca
     4381 gtcagcatcg atagaaacca tacgcgcgca ttgcatttct tcgtacatca tataaaactg
     4441 agtaaaaccg acggtgaagc aatctttttt ttcggggtat agtccttcga gaatacgaaa
     4501 gactttatgc acgccacggt cgttcatgcg gtaaaaattc gcacaacccg caatgagcgc
     4561 aggtaacatg tcggcgtacc acaagtgctt ttttggtttt tcaaaattga gtacttcaga
     4621 aaagtcggtg gcgatgcctt tgtcgtattc gatattgtgg taaagcatat cgcaaattat
     4681 attaccatat ttatccaatt tgttgaagag tttttcaacg ctgccattgg tatagctacc
     4741 gctactcgtg tattctaagt aattgctatg ctcgcgactc actttgagcg ttgcataagt
     4801 cgcgcagttt agacaactga ggaatacaaa acataggaca cccaaggaaa aaattgttat
     4861 gcggcgataa ataaaaaagc gagagtccat ttttctatga aggtcttgct gtctcgggtg
     4921 gtggctgcat ttcttttttc aagaaaggcg tcgtttccaa aagccttttc cgcattaaag
     4981 gagcaagcat atagtgattg cttacggtgc gctttacctt ccccggatat acttttacag
     5041 tcgtgttctt gcggatatcg ccgccgttac ccgaataata aggttcgtaa tggtttgtga
     5101 ccatgtctat gataacattc cccgtaggat tggaaaatcg gataattcca ttttcctctt
     5161 cgactccttt gagctctaac gcacggatca tggtggtttg cacggggtat gcatcgactt
     5221 tttcaatacg catgatgcta tagtaaaact ttctggggtc tcgctctact tctaatttga
     5281 tgtaagagag taaatcccac aatgctttat tgtattcgta attaccttcg atgtcgataa
     5341 accccttgat acccgagtac acccgttcgt cgaaaccggt tttcaaaatg tattcgttca
     5401 aaaatgcctt ggggtctttg gtttggcggt aattgagttt ttcgtcggtg agcaaacggc
     5461 gcgttaaaaa ttcaccgcgc ggcgtagtcg aattgctgta cgaaataaaa atgagcgggt
     5521 tcgctgtggg caaaacgcgc ttcaaaaatt caacaccacc gccattcaaa aacgcaggcg
     5581 tttcggtgaa aatgcgccct tcgggataat tcgtgcggtg gcatattggg tgcagtggct
     5641 tggggtcttt gagcggcgtt ttgctgtact tgtatttata ctcgtcgatg gtgatcagcg
     5701 ccgcaccggg caaatcttcc ataagattgc gcactccttt tgcaacagct gtgctgtgaa
     5761 agcccgacga ttcgccgcgc attgccacca tccacgtcgt gtctggacgc acgcgcttca
     5821 agtcgtggta gccaacaaca aacggatatc cattttgttc ggcgatataa aaccgccgct
     5881 cgtcgttgag cgtgtcgttg aaaacgtcga agtatggcgt ttcagaatgc cagggtcgcg
     5941 tcaccggtga atactcagaa caatcgaaaa accgtgcttt atcttttaaa gtaatgggat
     6001 gtttgtaaat ttcctcaaca ccgacgacaa cgggttttgt ttctttacgg tgtggtaatc
     6061 cgtcgttagt gggattcatt ttgttctgtg ttgtagagca gtagttaaat gcggtaatca
     6121 aaaaaaacaa aaagcacaat ctgtgcgtac gaaaaagtct attcatcgaa ggatagcttt
     6181 taaattaaga gtctatatgc caaccttaaa gttttgcaag cgttacttcc aaaatgcgct
     6241 tgcatccgct atcgacgcgc tttgtaaaag tcttgtaacc attcggcgag gcggtcgcct
     6301 gcaaattgtt tcatcgtcga ttcggctgcg agcacgactt gcgcgccctc ggtcatttgt
     6361 gcgccggaga taatatacgt tttgtcggct ttgagtaatt gcattttatt ttgcaaattt
     6421 cgcagcgaaa tatcgctgat gtgcgcgtcg ccacgcaggc ggtaaaaagc aaagagggcg
     6481 cggatagatt tactcttcga cgatatagca gaatagagaa ttaaatcttt ttcgcccgaa
     6541 ttttctttca ccggttcgtt atattccagc cgcgtaatga tataaagcac aaaacgctgg
     6601 aactcgtcgg gaggcatttg ttggaaaaga ttcgtgcctt tctcaggagt aacggcgtcg
     6661 tactcaatcc gcagcaccgg ttcgcctgcg acaaaatcaa aatactttgt aaaagcaatt
     6721 cctctatttt ttttgaaacc agaaactgta tacgttaaat cgttggggag taatccagaa
     6781 aaagtgtcgc ttaaaatttg cttaaacggc tttatcgatt cggtgtgggg gttcaaatcg
     6841 atttgatcga tacaagtttt taagtgcttc atgtcacggt agctttgttc gccgtcgtct
     6901 aaatcgcgca aaagacgcga ggcctctaaa aacatttctt cgtgcaaata aagtacaagg
     6961 aggatgtatt gatactccat ctttttttct tcggggagtt tattttgttc gttgcgctgg
     7021 cgtaaaaaat cgagggcttc gcgccatgct ttcgcttgat agcattgcca taaataaaga
     7081 tcaaaaagga gcgacaacga actcaggttt gtcgccgact ggataagttc gagcgacgct
     7141 tttttaccct gtgggagtcg agagcccgag gtggccatgg tcgcaaaaac cagtttgatg
     7201 gtttcgttgt cgggattact ctcgagtgct tgttccatga cgctcaacgc attgtcttgc
     7261 tcgccgcgtt gccacgaagc gacagcgtat gctaactgaa tttgtgcgtc gtctgagttt
     7321 tcgagagcgg ccgccagata tgtctcagcc acggcaaatt gaccgtcgcc taatgcaaga
     7381 agtcccactt cttggtttgc ctgctcgttt ttcgggtcga gttttagtat ttcaagcgag
     7441 gtctcaaaaa taccttcgta atcgccgtcg acgcggtaga gctcgataat gcgtaagaag
     7501 acctccacga cgcgctcgtt tgactcgaat gcgcctgcgt cgatggcctt gcggtaatgc
     7561 cgccgcgcca aatcgcgagc atccattttc tcgtggcact cggctaagta gacattcgcc
     7621 cgcgcgtagt ggtttggatt ttcagcaatt tcggttaagc gggtgattgc ttcgtcatag
     7681 ttgccttcgc tcatcagttt ttgagcgagg tgaaacgcat tgcttttgcg catagtcaaa
     7741 agccatgtca gcacgcctaa aagcgcaagg attcccagta aaacggcata aagcatgcgt
     7801 tattttatta tcgactaaag cagcgttgtg taaaccgaat tctttgtttt ttgttgaccg
     7861 tgacttctca ccctaagtat cttatcttta tggcgatgcc ttcgttactg cgcggtaaat
     7921 caaaggatga aacacaaaca atccattccc ttttggcgca cttgccttcg tatgtaacgc
     7981 gttcagaaaa agcattggtc aagaaagcgt atcttttagc cgaggatgcg cataaatttc
     8041 agaagcggct ttcgggcgaa ccgtatatca ttcatccctt ggcggttgcg catacattgg
     8101 cagatttggg tttagaccat gaagtcgttt gtgcgggatt attgcacgac gttctggaag
     8161 acacgtttat caaacccgat tatatgcgcc aagaatttgg tgaaacgatt acgtatttag
     8221 tcgaatcggt gacaaaaatt tctgtcttga aaagtcagtc tgccgaaaac aagcaagccg
     8281 tcaatattcg caaaatgatt atggcgacgg tgcgcgacaa ccgcgttatt ttaattaaac
     8341 ttgcggacaa attacacaac atgcgcacgc tgggttttca acccaccgca aaaggaatgc
     8401 acattgcgcg cgaagtgctt gaaatttatg caccgcttgc gggcagactg ggtatttata
     8461 aaattaaatc cgaactggaa gacctcgcgc tctaccattt agaccgcacc gcgtacgcga
     8521 ccatcaaaga acaagtcgct gaaaaaaaag ggcaacgcga cggacgggtg tcgatggtca
     8581 tgtcgctact cactacgcaa cttaaagaag caaaaatcaa agccaaagtg gaggggcgct
     8641 caaagcattt ttattctatc tataacaaaa tgcaagagca aaaaaaggac tttgaagatc
     8701 tttacgattt gaccggcgtg cgggttcttg t
//
LOCUS       DAOMPD010000041         8711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3467_length_8711_cov_2.324977, whole genome shotgun sequence.
ACCESSION   DAOMPD010000041 DAOMPD010000000
VERSION     DAOMPD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_8711_cov_2.324977"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /gene="nadB"
                     /locus_tag="PLY93_02410"
     CDS             complement(<1..1232)
                     /gene="nadB"
                     /locus_tag="PLY93_02410"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRP68364.1"
                     /translation="MRTEPHFLVVGSGIAGLFFALKASEYGTVTIVTKDAADNAATTY
                     AQGGIAAALGAWDDPQKHFEDTLVAGAGLCDEKAVDILVREGVSRVQELIDLGMPFTR
                     DKEGKLHFGREGGHNESRVLHDHDHTGRSVEFFLLERIAQKKSIRMFSNHCVVELATE
                     HHLKERKNSTMVFGAYVLDTATGEITLHKADFTLLATGGAGRLYPVTTNPQVSTGDGI
                     AIAYRAGCRIRNMEFFQFHPTALAIKGKNPPGTPTFLITEALRGEGAILKNPRTNRAF
                     MQDYHEMRELAPRDIVARAIDAELKRDGLDYVWLDISHKDAQFIQEHFPLISGTLKEK
                     YNIDMTKEAIPVVPAVHYTCGGVLVDYDGQTDLKNLFAIGEVASTGVHGGNRLASNSL
                     LEGLVFAARACDRIVKNI"
     gene            1390..1797
                     /locus_tag="PLY93_02415"
     CDS             1390..1797
                     /locus_tag="PLY93_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68365.1"
                     /translation="MVKAKIIEQLHKKRARPKNLPVTGKETKCKHCSGFEDKLSIAEQ
                     TEIKEEITLMQKHEIIRQMSMCMGADIECLQHAEKLFDQFKYHITEYVLKHEFNCESN
                     LSLQMVLWLEEKCCPDSAKSLHSVMGKYLHRDL"
     gene            complement(1805..>2395)
                     /locus_tag="PLY93_02420"
     CDS             complement(1805..>2395)
                     /locus_tag="PLY93_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRP68366.1"
                     /translation="GKIIGGAFTLLDASTALSTSTPLKGVFAGGRMPFVEDKVGPPSR
                     AYLKLWEIFTLTGFYPNEKMQALDLGATPGGWSYVAASLGARVTMIDRSAPDAALLKK
                     FPSLQFIKADGLKPTQDLLNESDVILSDMACDVTKLFDSVMRWILLDKVRFIACTLKF
                     HGVSDKVIIQKFAELPNSIIYHLWNNGHELTWIWQR"
     assembly_gap    2396..2405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2406..2758)
                     /locus_tag="PLY93_02425"
     CDS             complement(<2406..2758)
                     /locus_tag="PLY93_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68367.1"
                     /translation="MKDNYINKDKLQVGGACLYFIREELRAEFLSEVKFARVRVEEIY
                     PQVFLNKETTRLYWVLDYWPNLQEISFTSISDASKKIRAQGKATAYVGLNAFRRGALI
                     ADALKIKKYNTISFP"
     gene            complement(2748..3752)
                     /locus_tag="PLY93_02430"
     CDS             complement(2748..3752)
                     /locus_tag="PLY93_02430"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP68368.1"
                     /translation="MLWVVVVLVFIFLLGHYGLPEEVNPHAFQEMPAVSQLMRGAEPF
                     RLTGQTEVGFLLVHGFEDSPYIMRNLANLLHSEGHTVIAPLLPGHGTNIKAFERTRYE
                     HWRQMVLDTYLRERPRFKYFFMIGFSMGGNLTLNVASRVAKHAPPTGVITIAAPVTLN
                     GFLNGRLMLHDPRLFFTGILKYFVRYIPKSKGALTEKVMVPWVGYEEAYTLAPLHSFK
                     RGVSEIKHRLYRIVVPTCLIHAQNDKTVDVENMHYIFRRIRSPEKCAYVINLEENLST
                     HHVLVTHQIAREKVFHYIIKFVHDTLRGFDLKPALFKTKGDKKSWLQRLKRVRTIFEG
                     "
     gene            3895..5355
                     /locus_tag="PLY93_02435"
     CDS             3895..5355
                     /locus_tag="PLY93_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68369.1"
                     /translation="MRSWFISIALAVLTLHCVGSDALYDPPGGRGFWARRMDMSSGSS
                     TFYRLTAMYAGGSKHADVYTEVGQDVSGDIVDNLLAAFENAVVPIEHFWLRPPTDIDK
                     NDKVILLLLNIQDGYSTQGSFVGGYFDPYNNYSDESVNAVNSSYHSNEAEMLYLDTNP
                     WLQEAKRTQDFKAYYATIAHEYQHLLQFGTYFRGEKENFEPPWVDEGMAEVMADLTGY
                     GPQLGRANNFRRSLLQNTSLIKENATFSLENYAHSYIYFRYLADVYGLGIIGSIFKGT
                     HNGVPGVNTALEATDTKLLANCGNTATLPYPHFSCSYRFMWGAMVNNTLGDSPAGVRV
                     VFNGAASANLAVSGTYTYALTPANIEYKNELANSLATASIVDGNGDTGALESYAIKLY
                     KNNKTGAEPNFSLCAACRLTLVAGNKYFAVFNHDVSTATTASGRVSDTVHEAPQQPAA
                     GALPVVPEETQSNNIGAHKINLHFSIPQNLQEFLTP"
     gene            complement(5361..6161)
                     /locus_tag="PLY93_02440"
     CDS             complement(5361..6161)
                     /locus_tag="PLY93_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRP68370.1"
                     /translation="MRAALLQLNSQENLTQNFAMIDALLHEASLQNAEYALLPENFSF
                     FGSEKEKLVRQQEISEETILFLKNSAQKYKMIITGGGFPVPTGNGKVFNSALTLSPTG
                     EAIHRYDKLHLFDATPGDNATYRESHGAQAGNTIAPLLTIGEFSLGVTICYDLRFPYL
                     YRALALSGATAFLVPAAFTELTGKAHWHTLLRSRAIENTCYVLAAAQCGEHVNGRRTF
                     GHSMLVDPWGKIVKSLSDEPGTLVVDLSLPLVESVRKRMPSLLHGRSL"
     gene            complement(6178..7653)
                     /locus_tag="PLY93_02445"
     CDS             complement(6178..7653)
                     /locus_tag="PLY93_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68371.1"
                     /translation="MRQRKHFILLLYLFAATLFPLFPQNSAAKKNPPPIENTKVAPEE
                     IEEEHEGFPLFWQVSLREEPGYAMPRKSALLNPGDVLGLPRIQNRVLASLDAKVPIVG
                     GSGINPILEFVIADTFLYLQYEGHETVAKPTLSHAQNFLKEAYLNLRPASFWLMSAGR
                     RNVTDGVGYSRNLVDYLATPTTLPGFNLDNRVRIKNREGTILWRNEFFWKGGSFAYTY
                     VPSIQATAYNDVRVATEIDRITQFNRTDAHWLKVYEYIAGFDVSLHYFYRERHKAGLA
                     LAKVLGDALELHGEVNVQQGTNVLVPYKIQDDAYIGSTLVQAALYNYTERGNNTYYVR
                     SLVGGQYTFENKLNFSLEYYYNGEGYSSAEQKLLYDALGVSGARYNNPAFALGPKNPY
                     WLFLMAANQNFNYLSTGSHYVFMRLFNPEFFGSQKWEAALYTSVLVTDGSGLLAGDVT
                     YKWTDYISLQFLVNSFWGNKKSEGGLFYESFSALAALEAQF"
     gene            complement(7660..8394)
                     /locus_tag="PLY93_02450"
     CDS             complement(7660..8394)
                     /locus_tag="PLY93_02450"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRP68372.1"
                     /translation="MSILKKIAFILLAITGSALFAESASQIIRHADAVRGPAGSFQMK
                     VRVVSTRPDQDAQEMTLETYVKDRTTSVVKFLNPPREKGKAMLMVNDDLWMYIPGSKR
                     TFRISPQQRLMGEVSNADVARVNYADDYDAKILKTKEIVDGKTCYLLELNAKNKVSYY
                     RIHYWVEHKSYKPVKATFFAISGKPLKYATYTAYKKIAGATRPTVVHIIDGIKPDWKS
                     EIHYTSMGKKKFPASIFQQSALPDLR"
     gene            complement(8411..>8711)
                     /locus_tag="PLY93_02455"
     CDS             complement(8411..>8711)
                     /locus_tag="PLY93_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRP68373.1"
                     /translation="GAEGKAILKLFILEGILLGVIGGFIGVIIGAGIAFGINHANLVM
                     PPPPGSSQEFILQLRLYPTSFLIGFAVAVCSSFVASIYPALKAAKLRVVDALRHV"
BASE COUNT         2321 a         2098 c         2071 g         2211 t
ORIGIN      
        1 ttgatatttt tgacaatgcg gtcgcacgcg cgcgcggcaa aaacaagccc ttcgagaagt
       61 gaattcgacg ccaaccggtt accgccgtgg acgccggtcg acgccacttc gccaatcgca
      121 aataaatttt ttaaatcggt ttgaccatca taatcgacca acacaccgcc gcaagtatag
      181 tgcaccgctg gcaccaccgg tatcgcttct ttcgtcatgt cgatattgta cttttctttg
      241 agcgtaccgg aaatcagcgg aaaatgttct tgtataaact gtgcatcctt gtgtgaaata
      301 tcgagccaca cataatcgag accatcgcgt ttgagttcgg cgtcgatggc acgcgccaca
      361 atgtcgcgcg gtgcaagttc gcgcatctcg tgatagtctt gcataaacgc gcggttggtt
      421 ctagggtttt ttaagatggc tccttcgccg cgcaacgctt cggtgataag gaatgttggc
      481 gtgccaggcg gatttttccc tttgatggca agcgccgtcg ggtggaattg aaaaaattcc
      541 atgttgcgga tgcggcatcc cgcacggtac gcaatcgcaa tgccatcacc cgtcgaaacc
      601 tgtgggttcg ttgtcaccgg atacaaccgc cctgcaccac cggtggccaa cagtgtgaaa
      661 tcggctttat ggagtgtgat ttccccggtc gccgtgtcga gaacgtacgc accgaaaacc
      721 atcgttgaat tttttctctc tttaagatga tgctccgtcg ccaattcaac cacgcaatgg
      781 ttagaaaaca tgcggatgga ttttttttgg gcaatccgtt cgagaagaaa aaattcaacc
      841 gagcgccccg tatgatcgtg gtcgtgcaac acgcgtgatt cgttgtgtcc tccttcgcgg
      901 ccaaagtgca atttaccttc tttgtcgcgg gtaaacggca tgcctaagtc gatgagttct
      961 tgtacgcgcg atacgccttc gcgcactaaa atgtcgaccg ccttctcgtc gcaaaggcca
     1021 gcccccgcca cgagtgtgtc ctcaaaatgt ttttgaggat cgtcccacgc ccctaaagct
     1081 gcggcgattc ccccttgcgc gtacgtggtg gcggcgttat cggcggcgtc tttagtgacg
     1141 attgtcaccg tgccgtattc ggaggctttg agtgcaaaaa agaggccggc aatgccagag
     1201 ccgacaacca aaaaatgagg ttctgtacgc acaaagatag atttaaaagc gcaaacaggt
     1261 cgacgagaat ttactcaacc aattagttaa taaaaaaatt gtcacttggg ttcccaaata
     1321 gtatattttt ttcatccaac gccgaaagat cattggctat gagggatatt aaaaagatat
     1381 gagggtgaca tggtgaaagc caaaattatc gaacagctac ataaaaagag agccagaccc
     1441 aaaaatctac cggtaaccgg caaagagacc aaatgcaaac attgctccgg ttttgaagat
     1501 aaactcagta tcgccgaaca aactgaaatt aaagaagaaa tcactctcat gcaaaagcat
     1561 gaaatcatcc gccaaatgtc gatgtgcatg ggcgcagaca tcgaatgtct gcaacacgct
     1621 gaaaaattat tcgatcagtt caaataccac attaccgaat acgtactcaa gcacgagttt
     1681 aactgcgaga gtaatttatc gttgcagatg gtgctttggc tcgaagaaaa atgctgcccc
     1741 gattccgcaa aatcgctgca cagcgtcatg ggtaaatatt tgcaccgcga tctttaaact
     1801 tccctcaacg ttgccaaatc cacgtcaatt catgaccatt gttccataaa tgataaatta
     1861 tcgagttggg tagttctgca aatttttgaa tgattacttt atccgaaacg ccgtggaatt
     1921 taagcgtaca cgcgatgaat cggactttat cgagtaaaat ccaacgcatg accgagtcga
     1981 aaagttttgt gacatcgcac gccatgtcgc tgagaatgac gtcactctca ttgagtaaat
     2041 cttgcgtggg cttgaggcca tctgccttga taaattgcaa cgaaggaaat tttttaagga
     2101 gcgccgcgtc gggtgcgctg cggtcgatca tcgtaacacg tgcgcccaaa gacgccgcta
     2161 cgtagctcca tccgccgggg gttgcaccta aatccagcgc ttgcattttt tcattgggat
     2221 aaaaaccagt gagagtaaaa atttcccaaa gttttaaata ggcacgactc ggaggaccca
     2281 ctttgtcttc gacaaatggc atccggccac cggcgaatac acccttgagc ggtgtgcttg
     2341 tcgagagcgc tgtcgaggcg tcgagcaacg taaatgcccc accgatgatt tttccnnnnn
     2401 nnnnntacgg aaaagaaatt gtgttgtatt ttttaatttt gagagcgtcg gcaatcagcg
     2461 cgccacggcg aaatgcattt aaacccacgt acgcagtcgc tttgccttgc gcccggattt
     2521 tcttcgatgc gtcgctaatg gaagtaaagg aaatctcttg aaggttgggc cagtaatcta
     2581 aaacccagta aagtcgtgtt gtctctttgt ttaaaaatac ttgcgggtaa atttcctcga
     2641 cgcgtacgcg cgcaaattta acttcgctta aaaattcggc gcgcagttct tctcgaatga
     2701 aataaaggca agcgccgccg acttgaagtt tatccttatt gatataatta tccttcaaaa
     2761 atagtccgaa cgcgcttaag acgttggagc cacgatttct tatcgccttt agttttaaaa
     2821 agtgcaggtt tcaaatcaaa ccctcgcagc gtgtcgtgga caaatttgat tatatagtgg
     2881 aacacttttt cacgcgcgat ttggtgcgtc actaaaacat ggtgcgtact cagattttct
     2941 tcgagattta taacgtatgc gcatttttca ggcgaacgaa tgcgtcgaaa aatatagtgc
     3001 atattttcaa cgtccacggt tttgtcgttt tgagcgtgga ttaaacacgt cggcactaca
     3061 atccggtaga ggcggtgctt aatttcactg acccctcgct taaaagaatg caacggcgca
     3121 agcgtgtacg cctcttcata ccccacccaa ggcaccatca ctttttcagt gagcgctcct
     3181 tttgatttgg ggatataccg cacaaagtat tttaagatac ccgtaaaaaa aagacgcgga
     3241 tcgtgcaaca tcaaccgccc gttcaaaaaa ccgttaagag taacgggcgc agcgatggtg
     3301 atcacccctg tcgggggcgc atgttttgcc acacgcgacg ctacattaag agtgagattt
     3361 ccccccatcg aaaagccaat cataaagaag tacttaaatc gtggacgttc gcgtaaatac
     3421 gtgtcgagga ccatttgacg ccagtgttca taacgcgtgc gctcaaacgc tttaatgttg
     3481 gttccatgcc cgggcaaaag cggtgcaatg accgtgtgcc cttcgctgtg gagcaagttt
     3541 gctaaattgc gcattatgta cggcgaatct tcaaacccgt ggacaagcaa aaaaccaact
     3601 tcggtttgcc cggtaagccg aaacggctcc gcaccgcgca tcagttgtga tacagcaggc
     3661 atttcttgga aagcatgtgg atttacctct tcgggcaaac cataatggcc gagaagaaaa
     3721 ataaacacca aaacgacgac gacccacagc acaagggaaa actataaacg gcgagtctgt
     3781 gcgccaaccg taaagtttga ctgcctaaga ggctgtgcgt caacgagcct aagcgagttg
     3841 gcgcacagcc tcctaaaaga gttgactcgc cacaaacagt acattttatc gaatatgcgg
     3901 agttggttta tatccatcgc tttagcggta cttactctgc attgtgttgg cagcgatgcg
     3961 ctctacgacc ctcccggtgg ccgtggtttt tgggcacggc gtatggatat gtcgagcggt
     4021 agctcgactt tctatcgact caccgcgatg tacgctggtg gctctaagca tgcagatgtt
     4081 tataccgaag tcggtcaaga tgtctcaggg gatattgtcg ataacctact tgctgcattt
     4141 gagaacgctg ttgttcccat tgaacacttt tggctgcgtc cccccaccga catagataag
     4201 aacgacaagg tgattcttct tttgctgaat atccaagatg gctatagtac acaaggttcg
     4261 tttgtcggcg gctatttcga tccatacaat aactatagcg atgaaagcgt caatgcagta
     4321 aactcttctt accacagcaa cgaagccgag atgctctatt tagatacaaa cccttggctc
     4381 caagaagcca agcgcactca ggattttaag gcgtattatg cgacaatcgc acacgagtac
     4441 caacacttgt tacaattcgg cacatacttt agaggagaaa aagaaaattt tgaaccaccc
     4501 tgggtcgacg aaggtatggc agaggttatg gccgatttaa cagggtatgg tccccagttg
     4561 gggcgagcga ataattttcg ccgatcgctt ttacaaaaca catcgctcat caaagaaaac
     4621 gcaacgttta gtttagaaaa ttatgcgcac tcgtatattt attttcgtta tttggcagat
     4681 gtctacggtc ttggtatcat cggttcaatt tttaaaggga cgcacaatgg tgttccaggg
     4741 gtaaacaccg cgcttgaagc aaccgatact aagctattgg caaactgtgg caacaccgcg
     4801 accctcccct atccccattt ttcttgcagc taccgtttta tgtggggggc aatggtgaac
     4861 aatactttag gcgattcgcc cgcaggtgtg agagttgttt ttaacggcgc cgccagtgca
     4921 aacttggcgg tatcgggcac ttacacatac gcgcttaccc cggccaacat tgaatataaa
     4981 aacgaattgg ccaattcgct cgctaccgcc tctattgtag acggcaatgg cgacaccggt
     5041 gcgctggaaa gctatgcaat aaaactttac aaaaataaca aaaccggagc agagccgaat
     5101 ttttcgcttt gtgctgcgtg tcgtctaact ttggtcgcag ggaataaata ctttgctgtc
     5161 tttaaccacg atgtttcaac ggcaacgacg gcgagtggtc gcgtttccga caccgtacac
     5221 gaagcgccac agcaaccagc ggcaggagct ttacccgtcg tgcctgaaga gactcaaagc
     5281 aataatatcg gcgcacataa aatcaatttg catttttcga taccgcaaaa cttacaagaa
     5341 tttttaacac cgtaaaaatc ttacaaagaa cggccatgca agagcgatgg catacgtttg
     5401 cggacacttt cgacaagcgg cagcgacaag tcgaccacaa gcgttcctgg ttcatcggag
     5461 agactcttga caattttccc ccacggatcg acaagcatac tatgcccaaa cgttctgcgg
     5521 ccatttacgt gctcaccgca ctgggctgcg gcgagaacgt agcaagtgtt ttcaatcgcg
     5581 cgtgaacgca aaagagtatg ccagtgcgct ttaccggtaa gttctgtgaa cgcggctggc
     5641 accaagaatg cagtggcgcc cgataaagcg agtgcgcggt aaagataagg gaaacggaga
     5701 tcgtaacaga ttgtgacgcc aagagaaaat tcgcctatag ttaataaagg tgcgatggta
     5761 ttgcctgctt gggcgccgtg cgattcgcgg taggtggcgt tgtcgcccgg ggtcgcgtcg
     5821 aaaagatgga gcttgtcgta acggtggatt gcttcccctg tgggggagag tgtgagcgcg
     5881 ctattaaata ctttgccatt tcctgtcggt acaggaaatc caccgccagt gattatcatt
     5941 ttgtactttt gtgcagaatt ttttaagaac aaaattgtct cttcagaaat ttcctgttgc
     6001 cgtaccagtt tttctttttc actaccgaaa aaagaaaaat tttcagggag aagcgcatac
     6061 tcagcgtttt gtaaagacgc ttcgtggagg agcgcgtcta tcattgcaaa attctgcgtt
     6121 aaattttctt ggctgttgag ttgtaaaaga gcggcgcgca tatattttat acaaaaacta
     6181 aaattgcgcc tcaagcgccg ccagcgccga gaacgactcg taaaacaatc caccttcaga
     6241 ttttttattc ccccaaaatg agttcaccaa gaactgtaac gatatataat ccgtccattt
     6301 atacgtcaca tcgcctgcca agagtcccga accgtcggtg acgagaacgc tggtgtagag
     6361 cgccgcttcc catttttgtg aaccgaaaaa ctcaggattg aaaagacgca taaaaacata
     6421 atggctgccg gtcgaaagat aattaaagtt ctggtttgcc gccatgagga aaagccaata
     6481 ggggtttttg ggccccagcg caaatgcagg attgttatac cgcgcaccgg aaactcccaa
     6541 cgcatcgtag agcaattttt gttcagcgga tgaatagccc tcgccgttgt aataatactc
     6601 gagagaaaaa ttgagtttgt tctcaaaagt atattgcccg cccaccaacg agcgcacgta
     6661 ataagtgttg ttgcctcgtt cggtgtaatt atagagcgcc gcttgcacaa gagtggagcc
     6721 tatatacgca tcgtcttgta ttttatacgg gacgagtacg ttcgtgccct gttgaacatt
     6781 cacttcaccg tgcagctcca aggcatcccc caagacttta gcgagggcaa gtcccgcctt
     6841 gtgccgctca cgataaaaat agtggagtga aacatcaaaa ccagcgatgt attcatagac
     6901 tttgagccag tgcgcatcgg tgcggttaaa ctgtgtgatg cgatcaatct ctgtagcgac
     6961 tctaacgtcg ttgtaagctg ttgcttggat cgacggcacg tacgtgtaag caaaagagcc
     7021 gcctttccag aaaaattcat tacgccacaa aatagtgcct tcgcgatttt ttatgcgcac
     7081 gcggttatcg aggttaaagc ccggcagcgt tgtgggtgtg gcgaggtaat cgacgaggtt
     7141 acgcgaatag cccacgccgt cggtgacgtt acgcctgccc gcactcatga gccaaaacga
     7201 tgcgggacgc aagttcaaat atgcttcttt caaaaaattt tgtgcgtggc taagcgtggg
     7261 cttcgccact gtctcgtgcc cttcgtactg caaatagaga aaagtgtctg cgattacaaa
     7321 ctctaaaatg ggattgatcc cgctaccgcc aacgatgggc actttggcgt caaggctggc
     7381 cagcacgcgg ttttgtatgc gcggcaatcc caacacgtcg cccgggttta agagcgcgct
     7441 tttacgaggc attgcgtaac ccggctcttc cctgagtgac acctgccaga aaagaggaaa
     7501 tccctcgtgc tcttcttcaa tctcttctgg tgcaactttt gtattttcaa tcggcggagg
     7561 attttttttc gccgcactat tttggggaaa aagcgggaat aaagtcgccg caaataagta
     7621 aagtaaaagt atgaaatgct ttcgctgtct cacaaaatac taacgtaaat cggggagcgc
     7681 cgattgctga aaaatactcg cggggaattt tttctttccc atcgacgtgt aatggatctc
     7741 gcttttccag tcgggtttaa ttccatcgat gatatgcacc actgtaggac gcgttgcccc
     7801 cgcaattttt ttataggctg tgtacgtagc gtatttaagc ggctttcctg aaatggcaaa
     7861 aaatgtcgct ttgacaggtt tatagctttt atgctctacc caatagtgga tgcggtagta
     7921 actcacttta ttttttgcat tgagttcgag aagataacac gtttttccat cgacgatttc
     7981 tttggttttc aaaatttttg catcgtaatc gtcggcgtag ttcacccgcg ccacgtcggc
     8041 gttagaaact tcacccatga ggcgttgctg cggcgaaatc cgaaacgtgc gctttgaacc
     8101 ggggatatac atccacaaat cgtcgttgac catgagcatc gctttacctt tttcgcgcgg
     8161 cggattcaaa aatttcacca ccgatgtggt gcggtcttta acgtacgttt cgagtgtcat
     8221 ctcttgtgcg tcttggtcgg ggcgggtgga aacgacacgc accttcattt gaaatgaacc
     8281 cgcagggccg cgcaccgcat cggcgtgacg gataatttga gacgccgact cggcaaagag
     8341 cgcgctacct gtaatcgcca aaagaataaa ggcgattttt tttagtatag acatcttttc
     8401 tctccttaaa ttatacatgc cgcagtgcat ccacgacacg taatttggcc gctttgagcg
     8461 cggggtaaat cgatgcgaca aagctggaac acaccgccac cgcaaagcca ataagaaaac
     8521 ttgtggggta aagccgcaac tgcaaaataa attcctgact actgcccggc ggtggcggca
     8581 tcacgagatt tgcgtggtta atcccaaaag caatcccagc gccgatgata accccaatga
     8641 agccgccgat gactcccaat aaaattcctt cgagaataaa gagcttgagg atggcttttc
     8701 cttctgcacc c
//
LOCUS       DAOMPD010000042         8661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3496_length_8661_cov_2.754241, whole genome shotgun sequence.
ACCESSION   DAOMPD010000042 DAOMPD010000000
VERSION     DAOMPD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3496_length_8661_cov_2.754241"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLY93_02460"
     CDS             <1..616
                     /locus_tag="PLY93_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP68374.1"
                     /translation="KQMAIAGEFPDVVIGSHGGGSNFAGLAFPFLREKIHGKNVRVVA
                     VEPAACPTLTRGQYTYDFGDTVGLTPLVKMFTLGHDFMPAGIHAGGLRYHGASALVSQ
                     LVHEGLIEANAVKQNDVFASAITFARNEGILPAPESSHAIHQAVLEAKRADEEGKSRV
                     ILFNLSGHGHFDLSAFTQYLAGQMKDSTISEEDLKKGLATIPAV"
     gene            758..892
                     /locus_tag="PLY93_02465"
     CDS             758..892
                     /locus_tag="PLY93_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68375.1"
                     /translation="MQSKLEQFAVGQRATGLTALHAGLAEEFMQNSVQKFAKKIVSAS
                     "
     gene            945..1640
                     /locus_tag="PLY93_02470"
     CDS             945..1640
                     /locus_tag="PLY93_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRP68376.1"
                     /translation="MTISTQTQKIRLHGFPISNYYNRIKMALQLKGLSFEEVRTSPSK
                     ESEYLKINPLGVIPTLEIDGVFFAETHPTIEYLDEAYPDTTPLLPQDAIDRHRVRQIV
                     NYIDMYIDRPLRTLRDFQDKYSGASDALIEMTRTELEKGIESLNRTVRFKPYIAGNEI
                     SAADCAAYCTLPWFAYISEQYLGENLLHKIQGFDEYKAFLDQNNDFVAIHTAAAKAIK
                     LLERAKKFAGGKL"
     gene            complement(1891..3000)
                     /locus_tag="PLY93_02475"
                     /pseudo
     CDS             complement(1891..3000)
                     /locus_tag="PLY93_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
     gene            3266..4306
                     /locus_tag="PLY93_02480"
     CDS             3266..4306
                     /locus_tag="PLY93_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRP68377.1"
                     /translation="MEKKSLDDGILSLIIGVNPTERENRNDCHRQKRWLSYPIMRKIA
                     LFLLVSFTFFCKEKGKPVAEVKVHAVAIVFVSGTNANVVNASGTKKAVKGALVNQSDR
                     IITGAKSVVDLLLPNKIVVRIDQNTTVEMRTLTTSPDGAQNDKLYLQRGLIFAKVAKL
                     GQHSTFAIQTPTVVAGVRGTEFMTASDDKGGGKVAVTEGRVAVQSGENEHAVNAGEQA
                     SVDPAGKVSEGKIDADTLAQMKQFTTVADARAEMTQMLDDAIRFQQEALERAGGTDKV
                     KEIIEQNDKMIQEKKAETQQKIDAQKGETTNKIQEMKQGTDQKKDEMQNSIEEQRKKR
                     DELFNKNKFPQK"
     gene            complement(4418..5488)
                     /locus_tag="PLY93_02485"
     CDS             complement(4418..5488)
                     /locus_tag="PLY93_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP68378.1"
                     /translation="MQKVKVLVIEDSETIALLQTAILEKNNYNVKRAETGNEGLKLIA
                     EWQPEILILDIMLPDTNGLEILKDLSVLDIEKRPDTIVLSGHDDPKITFESLRLGAVD
                     FIPKPFHNEEYLLRVSKAAELRHYREANEALKAQLESDLRKLSHYFSRDLIHGILDGS
                     ISTKPGGEIVTASLLFFDMRRSTTLAEQMGPQNFFQFLSSFFADISDLIHSCNGVINK
                     FTGDGFLVTFGLRNYTQDATLNAMDCALRIRAHIAMYNEVRSTELPDPIGFGIGITTG
                     EVFAGNIGNVHKLEYTILGDPVNLAARLESMTKNAQLDILIDERTRKICGDSLKVKRL
                     KTRSVRGKTEEVTIYYPVLWSS"
     gene            complement(5504..5809)
                     /locus_tag="PLY93_02490"
     CDS             complement(5504..5809)
                     /locus_tag="PLY93_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68379.1"
                     /translation="MHENNRIEVTQTPWQNCIAVCSKCSRKLNAMDKDKTCLRAGLKA
                     LIKERGLKNKVRAVDSSCFDICPEGRIVVVHFTENGITPITVTTDTAPTEVLSQFGY"
     gene            5968..>8661
                     /locus_tag="PLY93_02495"
     CDS             5968..>8661
                     /locus_tag="PLY93_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP68380.1"
                     /translation="MERKSFRKRIADTNRDITEEITPPSQTKEKKKQAILIQGWIAAL
                     ERGGVAAFSVKNPYLMLVICIMITILGGLAVFGMPKDLLPPSKQPAVQILSLYPGMAT
                     PNIETNLTYKFERYTGQAVGIERQESRSIPGVSVIKNFFDEKVNDQNSVMATTTGLIM
                     SVLRRLPPGAQPPIILPFDPMGAVPLTLVTVSGDFPVNKIYDFAQYYVRRDIQTNPGA
                     VAPTQIGGAEKQVILRLDPQKLQYYNMSASEVLDKVSRFNTFIPAGNVKIGKFDYQVY
                     TNGIADTIDSFNAFPLRTQEGVHVYLRDVGQAIDSNIVQTNVVTVNGKEQVYVPVFRQ
                     QGANSLDVVERTRKAMKTLEGEIGVKLDLIADQTVFIKHAIDAVGHEALLGGCLAALM
                     VFLFLGNPRAAGAVLLAIPLSALFTFMGLKVLGQTVNLMTLGGIALSIGLLVDNAIVA
                     IDNIMRHLRENDHPNRVQVIVSAVHEVTGPIISITLCILVVLLPLLLTSGVINVLFGA
                     IALTVMLAITGSLIAVATVIPLFASRYLSSASKVLPRILARIQEGLLKLSDAYARLLD
                     KVMHRLGWVVGAVSVLLVGGILLTPFIGSELFPRADAGNLVVEARFQSGLRIEETKKK
                     SIELENKIREFIDATDLKMVLANIGVYNGYSAAFTENSGTQDVTMTIELSENRKHTSQ
                     YYAKILRHKLPKLFPDMDLGIQLGGILTSALNGGSKAPIDIQIEGKDVLVSHKIAEEM
                     LPKIKALRGAVDVRIQERFDAPGIEVNINRVKADERGIYADEIVQNVVSSSTGSITYR
                     PSVWVDPKTGIDYFMGVRFPEREMSSRTAFGNIPITGQRQLRSITLKNLAEIKDINSI
                     TELSRVNMKRVINIWLDAEVRDVGSLSRAIQKQVINKTQFPE"
BASE COUNT         2348 a         1974 c         2079 g         2260 t
ORIGIN      
        1 caaacaaatg gccatcgcgg gtgaattccc cgacgtggtg attggttcgc acggtggcgg
       61 cagcaacttt gccggtttag cgttcccttt cttacgagaa aaaatccacg gcaagaacgt
      121 gcgcgtggtg gcggtggagc cggcagcatg ccccacactc acgcgtggtc aatacaccta
      181 tgattttggc gacacagtag gcttaacgcc gttagtaaaa atgtttacat tgggtcatga
      241 tttcatgccc gcaggaattc atgccggtgg attacgctac cacggcgcat cggcgctcgt
      301 gtcgcagtta gtccacgaag gtctcatcga agcgaatgca gtgaaacaaa acgatgtgtt
      361 cgcctcggcg atcacgtttg cgcgtaacga aggcattttg cccgcacccg agtcgtcgca
      421 tgctattcac caagcggtgc tcgaagccaa gcgtgcggac gaagaaggta aaagccgcgt
      481 gattctcttc aacctttctg gccacggcca ctttgacttg agtgcattta cacaatacct
      541 cgccggtcaa atgaaagact cgaccatcag cgaagaggac ttgaagaaag gtttggcgac
      601 aatcccagca gtataaagct tgctgtcaaa gtgccgtgtg tagggagcct gtgcgccaac
      661 tcgcgcagcg tgtacgaata agcaattttt ttgcaaattt ttgtacgcgg ttcgctcaaa
      721 aacgcaaaat tttgcataaa ttcgggcgtc ttcccccgtg cagagcaaat tggaacaatt
      781 tgccgtcggc caaagagcca ccggcctcac ggcattgcac gccggtttgg ccgaggaatt
      841 tatgcaaaat agcgtacaaa aatttgcaaa aaaaattgtc tcagcgagtt agcgcatagg
      901 ctctaagcgg ttgccgcttt gactcggcac taccatgcgc cactatgacg atctctactc
      961 aaacacaaaa aatacgattg catggctttc ccatcagtaa ttattacaat cgcatcaaga
     1021 tggcgctcca actcaaggga ctttcctttg aagaagtgcg tacatcgccg tcgaaagaaa
     1081 gcgagtactt gaaaattaac ccactcggcg ttattcccac attagaaata gacggtgtct
     1141 tttttgcaga aacacacccc acgattgaat acctcgacga agcgtatccc gacacgacgc
     1201 cgcttttgcc acaagacgcc atcgaccgac accgtgtacg gcagatcgtc aactacatcg
     1261 atatgtatat cgatcgaccg ctacgcacat tgcgcgattt tcaagataaa tactcgggtg
     1321 catcggacgc tttaatagaa atgacgcgta ccgaactcga aaaaggcatc gagagtctca
     1381 atcgcactgt gcgtttcaaa ccgtatatcg ctggcaatga aattagcgct gccgattgcg
     1441 cggcgtactg cactttgcct tggtttgcgt atatctcgga gcaatactta ggtgagaatc
     1501 tgctccataa aattcagggg ttcgacgagt ataaagcttt cctcgatcaa aacaacgatt
     1561 ttgttgcgat ccacacggcg gcggcaaagg cgataaaact cctagaacgc gcaaaaaaat
     1621 ttgcgggggg taagttgtaa tcttggatga tgtctatggc cttaatgcgg tgaaagaacg
     1681 gtatccgaat tgtctgaaag tgatttcttc ttcaaacgtg gttccctttt ttgaccactt
     1741 cagcgctcgc ggtggatagt taccccttaa tgtgacataa tctgaaaaaa ttgggatagt
     1801 taccccctaa tgtgacataa tctgaaaaat taggatagtt accccctaat gtgacataat
     1861 ctgaaaaatt aggatagtta tccccaccga ctactgagca acccgtggca gcgtggctaa
     1921 gtattgttga atttccggat cgttgtcttt ggtgagctgc caaaaagaaa atcctgcgag
     1981 gtggtgcttt tgcaaaagtg ccatcttatc ggcaaacgca gttttatcca taaagaaagc
     2041 gacgcgctgg cacgagcctg cggtatacca cagcgacggg tcttcataaa ttttatcttt
     2101 gtggacgcta cggaatttac gtaaattgcg ccatgattta gacgccttca cgctatctaa
     2161 aattttggtg atgttcgttg gttggcgcag tgtgtgcgca cctgctttga tgcgtaatac
     2221 atcgtgataa taaaccgact tcacgcgcga ggaacagttg agcccccaat cgtatccata
     2281 cgtcggtatt gccatataga gttttgacgc cgggatttta tcttgtgcgt acgctataat
     2341 ttcttcgagc cacacgtttg gtgcttgggg tcctggcccg gggttgcggt atttgcgcgg
     2401 gtgtagttcg tacgccataa tttttaccat atcggtgtgc ttacccaaaa atgcgtagtc
     2461 gtgcgtcaga gggccgcgcc aatcttcacg aaagtccatt gtgatggggt ttttcaatcc
     2521 tttacattct tttgtatcgg cttcttcgta ttcaatgggt gtcttaggat gtaccgcgac
     2581 cgagagcaat tttttacgcg cgtgtaattc agtcgagagc ttgagaataa actcttcaaa
     2641 tttttctttt ttaccgcagc tcatcccttc gtagtcgata tcgatgccat cataaccgta
     2701 tgtttccacc tctttcacaa tgatgccgat atgtttgtcg cggccattca tgccaatcgc
     2761 ttcttgaatt ttttcgccgg ggttttccca acgaaaaatg gtggggataa ttttcaaatt
     2821 tggatttttc gttctaagcg cttggacgcg ttgtagtttt tgggtatgcc cccacgacga
     2881 aactaagtcg cctgtgttgt gcaaaccgcc tttcaagcca taaataaagg gatgcaactc
     2941 gtcgtagagg tgcgcgtatt tgagcatcac atcgtaatcg aacgaccacg atgaggcacg
     3001 cagcggtcca ggtttgcctt gaggtggagc cacagtggga tttaccccgt taccgctttg
     3061 ggtgttttcg gttgcagtta ttgctttatc ggtcgtcacc tgcggcgtac cgccaatcat
     3121 acgaatggtt atggagaggg taacggcgag cgttaagaga gaaatccaaa atggccatgt
     3181 acgccgcttg ggtttatttt cttcggggcc tttctcttcg gggggtggtt ctgttggatt
     3241 attttcgacg gggagcacgg gttcaatgga aaaaaaatct ttagacgacg gcatactatc
     3301 gttgattatc ggtgtaaatc ccactgagcg agagaataga aatgattgcc accggcaaaa
     3361 gcgttggtta agttacccta ttatgagaaa aatagctcta tttcttcttg tgtcttttac
     3421 atttttctgt aaagaaaagg gaaagcctgt tgctgaagtg aaagtgcatg ccgttgctat
     3481 tgtgttcgtt tcgggcacaa atgcgaatgt cgtgaacgct tccggtacaa aaaaggcggt
     3541 caaaggtgcg cttgtcaatc aaagcgaccg catcataacc ggtgcaaagt cggttgtcga
     3601 tttacttcta cctaataaaa ttgtcgtgcg tatcgatcaa aacacaacgg tggaaatgcg
     3661 tacgttaaca acatccccag acggcgcaca aaacgataag ctctatttac aacgtggatt
     3721 aatttttgcc aaggtagcaa agttgggtca gcactcgaca ttcgcgattc aaacgcccac
     3781 cgttgtggcg ggggtacgcg gtactgagtt catgactgca agcgacgaca aagggggcgg
     3841 caaggttgcg gtgaccgagg gccgagtggc ggttcaatcg ggtgaaaacg aacacgcagt
     3901 gaacgcgggc gagcaagcaa gcgtcgaccc agcgggcaaa gtttccgaag gtaaaatcga
     3961 cgccgacacg ttggcgcaaa tgaaacaatt taccaccgtt gccgacgctc gtgcagaaat
     4021 gacacagatg ctcgacgatg caattcgatt ccaacaagaa gcgttagagc gcgcaggcgg
     4081 caccgataaa gttaaagaaa ttatcgaaca aaacgataaa atgatccaag agaaaaaggc
     4141 agagacccaa caaaaaatcg atgctcaaaa aggcgaaacg acaaacaaaa ttcaagaaat
     4201 gaaacaaggc accgatcaaa agaaagacga aatgcaaaat agtatcgaag agcaacgcaa
     4261 aaagcgggac gaactcttca acaaaaacaa gttccctcaa aaataaaagt caagaaagat
     4321 aattaaggct gtctaataag tcgctggtgc gcccaaaggc gcatcgcgcc gctggacagc
     4381 ccaaaaaaat gcaatatttt cgactcttta gataatctca actactccaa agaacaggat
     4441 agtatatcgt tacttcttct gttttgccac gcacgcttcg cgtctttaat cgttttactt
     4501 tgagcgagtc gccacaaatt tttcgtgttc tttcatcgat gagaatgtcg agttgcgcat
     4561 tctttgtcat cgactctaac cgcgccgcta aattgacggg gtcgcctaaa atagtatatt
     4621 cgagtttgtg gacattccca atgttgcccg caaaaacttc accggtggta atgccaatgc
     4681 cgaatccaat ggggtcgggt agctctgtcg agcgcacctc gttgtacatg gcgatatgcg
     4741 cacgaatcct caatgcgcaa tccatcgcgt tcaaggtggc gtcttgggtg taattacgca
     4801 ggccgaatgt caccaagaaa ccatcccccg taaacttgtt gataacgccg ttacaactgt
     4861 ggattaaatc ggaaatgtcg gcgaagaaag aactgaggaa ttggaaaaaa ttctgcggcc
     4921 ccatttgctc ggccagcgtt gtcgagcggc gcatgtcgaa aaaaagaagc gacgctgtga
     4981 caatctcgcc gccaggtttt gtagagatgc tgccgtctaa aataccgtgg attaaatcgc
     5041 gcgaaaaata atggctcaac ttacgtaaat cgctttctaa ctgcgccttg agcgcctcgt
     5101 ttgcctcacg gtaatggcgc agctcggcgg cttttgagac ccgcaataaa tactcttcgt
     5161 tgtgaaaagg tttaggaata aagtcaacgg cacccaagcg caatgactca aaagtaattt
     5221 taggatcgtc gtggcctgaa agcacaatcg tgtctggtct tttttcaata tcaagcacgc
     5281 tcaaatcttt taagatttca agaccgttcg tgtcgggcag catgatatcc aaaattaaaa
     5341 tttcgggttg ccactcggcg ataagcttca acccctcatt acctgtttcg gcgcgtttta
     5401 cgttgtaatt atttttttca aggatcgctg tttgtagtag agcgatcgtt tcgctatctt
     5461 caataacgag tactttaact ttttgcatag ataggactag ctcctaatat ccaaactgtg
     5521 ataaaacttc agtcggtgcg gtgtcggtag ttaccgtgat gggagtgatt ccattttccg
     5581 taaaatggac tacaacaata cgcccttcag ggcaaatatc aaaacaggaa gaatcgacag
     5641 cgcgtacttt gttttttaga ccacgttctt taatgagcgc tttcagtccc gcacgtaggc
     5701 aagttttatc tttatccatg gcgttgagtt tacgcgagca ctttgaacag acggcgatgc
     5761 aattttgcca tggcgtttgg gtgacttcaa ttctattgtt ttcgtgcatt gttgaacgct
     5821 attcttctaa ggataaataa taagaaaaac atcgatagaa ttctataaaa aatagaaaaa
     5881 gcgtatccat tcggggaagt taaaattttt ttgcagtcct ttgagtaaaa aaggacaggg
     5941 ggaccagtgt ttggccgaat ataatatatg gagagaaagt cctttcgtaa gcgcatagcc
     6001 gataccaaca gagatataac agaagagata actccaccat cgcagacaaa agaaaaaaag
     6061 aaacaagcaa tccttatcca agggtggatt gccgccttgg aacgcggcgg cgttgccgcc
     6121 ttttcggtaa agaatcccta ccttatgtta gtcatttgta tcatgattac tatcttaggc
     6181 ggtttagccg tctttggtat gccgaaagat ttattgccac cctcgaaaca acctgccgta
     6241 caaattcttt ccctatatcc aggcatggca acccccaaca ttgaaacgaa tcttacgtat
     6301 aaatttgaga gatacactgg tcaggcggtg gggatagagc ggcaagaatc gcgttctatt
     6361 ccaggagtaa gcgttataaa aaattttttc gacgaaaaag ttaacgacca aaactcggtg
     6421 atggcgacaa caacaggact tattatgtcg gtgctgcgtc gtttgccgcc cggcgcacaa
     6481 ccaccgatta ttttaccctt cgatcctatg ggagcagtac cattaacgtt ggtgacggtg
     6541 agcggggatt ttcccgtcaa taaaatttac gactttgcgc agtattatgt acgccgcgac
     6601 attcaaacca acccaggcgc cgtcgcaccc actcaaattg gcggagcaga aaaacaggta
     6661 atcctacgac ttgatcccca aaaattgcaa tactataata tgtcggcgtc agaagtgctc
     6721 gacaaagttt cacgcttcaa tacgttcata cctgctggca atgtaaagat aggtaaattc
     6781 gattaccaag tttacacgaa cggtatcgcc gacactatcg atagtttcaa tgcattccca
     6841 ttgcgtactc aagaaggcgt gcatgtgtat ttgcgagacg tgggccaggc gatcgactcg
     6901 aacattgtgc agacgaatgt cgtgacagtg aatggtaaag aacaggtcta cgtaccggtg
     6961 ttccgtcagc aaggagccaa ttctctcgat gtggtggagc gtacgcgcaa agcgatgaaa
     7021 accctcgaag gtgaaattgg agtaaagctc gatttgattg cagaccaaac tgttttcatc
     7081 aagcacgcca tcgatgcggt gggacacgaa gctctcttgg gtggatgtct tgcggcgctg
     7141 atggtctttt tatttttagg taacccacgt gcagccggtg cggtgctttt ggcgataccg
     7201 ctttcagcgc ttttcacatt catgggattg aaagtattgg gacaaacagt caatctcatg
     7261 actttaggcg ggattgcgct ttccattggt cttttagtcg acaacgcgat tgtggcaatc
     7321 gataatatta tgcggcattt gcgcgaaaac gatcacccca accgcgtcca agttattgta
     7381 agcgcggtac acgaggtgac aggtcctatt atttcaatca cgctttgtat tttagtagtg
     7441 ttgttgcctc ttctcttaac ctctggagtc atcaatgtac tttttggtgc aattgcgctc
     7501 acggtgatgc tcgccatcac aggctcgctt attgcggttg ccacagtgat tcctcttttt
     7561 gcgagccgtt atctttcgtc ggcatcgaaa gttttaccgc gtattttggc gcgcattcaa
     7621 gagggcttgc tcaagctcag cgatgcatac gcacgtctct tagataaagt gatgcatcgt
     7681 ttaggttggg ttgtcggcgc cgtttcggta ttgcttgtcg gcgggatatt gctgacccct
     7741 tttataggtt ctgaactttt tccgcgcgcc gatgccggaa atctcgtcgt cgaagcgcga
     7801 tttcagtcgg ggttgcgcat cgaagagacc aaaaagaaaa gtatcgaatt agaaaataaa
     7861 attcgcgagt tcatcgacgc taccgatttg aagatggtgc ttgccaacat cggtgtgtac
     7921 aatggctatt ccgctgcgtt caccgaaaat tcggggacgc aagatgtcac aatgacgatt
     7981 gaactctctg aaaatcggaa gcatacgtct cagtattacg caaagatatt acgccataaa
     8041 ctccctaaac ttttccccga tatggattta ggtatccaat taggcggtat tttaacgtcg
     8101 gcgctcaacg gtggttcaaa agcgcccatc gatatccaaa ttgagggcaa agatgtttta
     8161 gtgtcgcaca aaattgccga agaaatgtta cctaaaatta aagcattgcg cggcgcagtt
     8221 gacgtgcgca ttcaagaacg cttcgacgca ccgggtattg aggtgaatat aaatcgagtg
     8281 aaggccgatg agcgcggcat ttatgccgat gaaattgtac agaacgtagt gagctcgtcg
     8341 accggttcga ttacataccg ccccagcgtg tgggtcgatc ctaaaacggg gattgattat
     8401 tttatgggtg tccgttttcc tgaacgcgaa atgagttcac gcaccgcgtt tggaaatatc
     8461 cccatcaccg gtcaacgaca gttgcgatcg attactctaa aaaatttggc cgaaattaaa
     8521 gacatcaata gcatcactga gttaagccgt gtcaacatga agcgcgtgat caatatatgg
     8581 ctcgatgccg aagtgcgtga cgtgggctcg ctttcgcgcg caattcaaaa acaagttatc
     8641 aataaaaccc agttccccga a
//
LOCUS       DAOMPD010000043         8594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3532_length_8594_cov_3.339150, whole genome shotgun sequence.
ACCESSION   DAOMPD010000043 DAOMPD010000000
VERSION     DAOMPD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_8594_cov_3.339150"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLY93_02500"
     CDS             complement(<1..636)
                     /locus_tag="PLY93_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68381.1"
                     /translation="MIDCNNYHLADKLENPGNTTEANKCARDSIAAITLTTPGTNYTS
                     PPTIAISGSGSGAQAVAYLTPTSVGSFTVVNAGSGYICPFAGSITVSGGGGSGASATG
                     TTGCSITGVVLGSGGNGYVAPSFTVTFTGDGTGANAVATVPIPSGPITAVTLGALGSG
                     YTGMPMPMPDFSAGGGGSGATLTVILSNGLLQSVTGSGGSGYTSPPTVTVSG"
     gene            1486..1800
                     /locus_tag="PLY93_02505"
     CDS             1486..1800
                     /locus_tag="PLY93_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68382.1"
                     /translation="MEKSISNFVSFGIGAFESARQSVETLLKNLETEVNALIAQGETE
                     DSENVVRVRAAVTDASTQLMTFSERLKLEYSELNGRVQETLTAIQAHAKDLAASLPKR
                     SA"
     gene            1858..2739
                     /gene="sucD"
                     /locus_tag="PLY93_02510"
     CDS             1858..2739
                     /gene="sucD"
                     /locus_tag="PLY93_02510"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRP68383.1"
                     /translation="MAVLITEKTRIIVQGITGKEGSFHTGQMLEYNTRAKVVGGVTPG
                     KGGQKSEHGLPIFNSVKESVEKEQANAAVIFVPPPFAADAILEGISAGLALVICITEG
                     IPVRDMSRIVDFIRNSKTVVVGPNCPGVITPGVGKMGIMPGFIHKPGSIGVVSRSGTL
                     TYESVHQLTQVGLGQSTCIGIGGDPIIGLPHLQAVKLLAEDPATEGIVMIGEIGGSAE
                     EEAAEYIKAHVKKPVVGFIAGQTAPPGRRMGHAGAIISGSAGTAQTKMQAMKDAGIHV
                     CESPAQIGETMKKALGK"
     assembly_gap    2800..2829
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2830..3229
                     /locus_tag="PLY93_02515"
     CDS             <2830..3229
                     /locus_tag="PLY93_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP68384.1"
                     /translation="GKTLTPADIQGKLVLVSFFFSRCSGICPMVMACVWCIYQRITDH
                     SNLIFVSVSVDPERDRKLQLAEFRTRMLGKDGLPGKLPFKNWYFTTGDKTEIYALARG
                     VFQADGLCATQKKANHAIWRIFYTLSRFIY"
     gene            3486..4391
                     /locus_tag="PLY93_02520"
     CDS             3486..4391
                     /locus_tag="PLY93_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRP68385.1"
                     /translation="MGTIAKPFLKWAGGKTQLLPEIIKRLPANIKNQGFTYIEPFVGS
                     GAVLFWMLGHFDNLERAVINDINEDLVNTYRVVAQEPYELIKLLQTFQIEFHRLENND
                     LAKKKYYYQKRELYNSRRSDTIKQAALFIFLNRTCFNGLYRVNKQNEFNVPMGSYKKP
                     TICDSENLLAVNKILQKVKILCGDFENTLAYAHENTLFYCDPPYKPLNLTSSFNSYAK
                     DEFGDAEQIRLSNFCKRLEELNHAWILSNSDVSVVESNNFFNELYGKFEIQKVNARRN
                     INSISTKRGEVKELLITGNMAFANS"
     gene            4388..4735
                     /locus_tag="PLY93_02525"
     CDS             4388..4735
                     /locus_tag="PLY93_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68386.1"
                     /translation="MIFGGKGGGNTRTGLVFEGNTDLADFLNKQKNYKVQNGDVLYKE
                     EKIVRIFKKYSFYKFLDELKIDWCSRISKRLLPDDSIFVIIANTLFIIECKFQRVAGS
                     VDEKLQTCDFKRK"
     gene            4906..5727
                     /locus_tag="PLY93_02530"
     CDS             4906..5727
                     /locus_tag="PLY93_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRP68387.1"
                     /translation="MKNLHYESQDGMFRLFQNDSLEALSTINEKFDMVFVDPPYFLSN
                     NGLSIQSGEIVSVNKGAWDKSPGNAEIYTFNRAWITAVRETMKNDATIWISGTMHNIF
                     SIGQILQELRFKVLNVITWEKTNPPPNFSCRYFTYSTEFVIWARKSAKEKHYFNYDLM
                     KKIAGDRQMKDVWKLSAIQSWEKTCGKHPTQKPLALLTRIILASTKPNAQILDPFTGS
                     STTGIAANLAGRIFVGIDKEKDFLEISRRRKIEIEDTRIAHLYRDRINDYYALRS"
     gene            complement(6238..>7312)
                     /locus_tag="PLY93_02535"
     CDS             complement(6238..>7312)
                     /locus_tag="PLY93_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68388.1"
                     /translation="DIFPTLHTIDKPFREEIKEAPQELEKMEERNEIIENYRLWREGK
                     RIADKNRTKVQVNRQAKGSAGKLNFNAAERRKYFDETLSDPKEKQLDSFLRDYGNYDP
                     NEGDLPQAIRDLYYENLQRVASSFNSDMTYLAIDYFHEALNKEEFLRKALDVVSKKKG
                     TKYATEILFAIENIYEIQGRALKLLFDVKKHIDRLSPKERDNIRIKTLEKVVKRYLPL
                     AEKKGIRNEDAALQHYYLKRLEILDYLVETSPGGYRSKDATLAKGRIFWESIDPANPN
                     SWQNGNRAVDIWRTIDNLADDGDFIGEDLYKSMKEHLKRYRSLNSIESQRIQQALRQN
                     DTKLLQKKIERENRILWSSKNNK"
     assembly_gap    7313..7322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7323..7786)
                     /locus_tag="PLY93_02540"
     CDS             complement(<7323..7786)
                     /locus_tag="PLY93_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68389.1"
                     /translation="MAAFLKDSFLHFLGRQRWSLFLYLSAILHLSLFTHLWIKGLKTQ
                     EIEICNQPQISLMAQVTPSPKSPHSQEGDQKGEDGGGFNNGPMADAQGKGEGDFAEKF
                     GLKGDKWKEQIDRTEEGETLNDSYSDKDNEKGAINNDVPEEYRKRKRDYRNI"
     gene            complement(7817..>8594)
                     /locus_tag="PLY93_02545"
     CDS             complement(7817..>8594)
                     /locus_tag="PLY93_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68390.1"
                     /translation="NPDDASQTYLYVVDYGGKRISRRNRLTGAYGGFIGNGENAWNTT
                     ATTQSPAYGHTAGYLGYGSIGPRGVAIANGKLYVVDEGNQRIVRIDAASGNFERWLGS
                     GYDDWQVMTTAPNQTGNSAVKYFRYPSAVVTDGTYLYVADRGNNRIVKWRIDGLNCDK
                     PISSGKFCGWIGHGRVGWETTASAPSTNPADDPYVGSYYPSDYYAQPHGLVLVKASQK
                     GTRNNYLFMTSVYNGRVSRINLDCVDTPSGSECDPIYTIP"
BASE COUNT         2287 a         1953 c         2016 g         2298 t
ORIGIN      
        1 gcccgaaact gtgaccgtcg gcggtgaagt ataacccgag ccccctgaac cggttaccga
       61 ctgtagtaaa ccattactta aaataacggt cagtgtcgcc cctgagccgc cgccgccagc
      121 cgaaaaatca ggcattggca tcggcatgcc tgtatagccg ctgcccaatg cgccgagcgt
      181 gaccgcggtt attgggccag aggggatggg cacggtcgcc accgcatttg ctccggtacc
      241 atcgcctgta aaagtaacag taaaacttgg agcaacatat ccgttgccac cgcttccgag
      301 aaccacaccc gtgatactgc aaccggtggt tcccgttgcg gacgcgcccg aaccaccacc
      361 gccactcacg gtaatggaac ctgcgaaggg acaaatatat ccgctgccag cgttcacgac
      421 cgtaaaactg ccgacagatg tgggcgtaag gtacgcgaca gcctgcgctc cgctgccgct
      481 accggaaatc gctatcgtcg ggggtgacgt gtagtttgtt cccggcgttg tgagcgtgat
      541 tgcggcaatg ctatcccttg cgcatttatt cgcctccgtg gtgtttccgg gattttccag
      601 tttatcggct aaatgatagt tgttacagtc gatcataaag caaattaaca gcgtcattaa
      661 gcatatcaca taatgaattc gacagccgta atatttttgt cgatcaatca tcaacacctc
      721 cgagttatag tgtcataagc gctgaggcac acgcgaactc aagaaaactt atgcacttgt
      781 tcggccgaat acgcttgcaa ggtatggcca gcaacgatgg atttacctga cgttgatggt
      841 gcgccatcga tgtaccgcag gtggctccgt tacgtttgtt aatgaacctc aacagactac
      901 aggtgtcaac gtatttcaag tcaccgcacc cgatccgcaa accaacgagg agcctgtaca
      961 ccaactcgcg cagcgtgtac gaaaaagaaa tcccgggcat tgtgttgtta aacatagtag
     1021 atttacctgc tgcgccgtga gtttccggaa aactatgcgg tcgtatccgt gcatgtaaac
     1081 aggctcaatg attttctcac caccacccgc ttctgcttgt atcatgaagg cgccgaacac
     1141 ctacagccaa gctggggagt aaagttgctg cagtgtagat catatttggg acagctatct
     1201 cactgctatt cataatcatg ctacccaaca gcattaccag aaaagaaact ataatatttg
     1261 ctaagaagaa catccaatac tcgcgccgcc gcacccggca agtttactat aaagtgtaac
     1321 ttgccctcgc tggacgacga cgccgggctc ggaagacaga gactcgccta cggctcattg
     1381 ctcagacacc tcagcagagt aaatttaact tgccaccatg tgcgtcgcac tatatacttg
     1441 atatgacttg ttgctttgcg aaaaagcgac catgaaggag tggcgatgga aaagagtatt
     1501 agcaattttg tgagctttgg tattggcgct tttgagtctg cgcgacagag tgtggaaacc
     1561 ttactaaaga acttggagac cgaggtgaac gccctcattg cgcaagggga aaccgaagac
     1621 agcgaaaatg tcgttcgggt acgagccgca gttaccgacg cctctacaca attaatgacg
     1681 ttttcagagc gcctcaagtt agagtatagc gagctcaacg gcagagtcca agagacttta
     1741 actgccatac aagcacacgc caaagactta gcagcatcct taccaaaacg cagcgcataa
     1801 aagcttgagt acaagttcct ttacccgtgg tctgttcttc ggtgtttgtc cctaatcatg
     1861 gcagtactga ttacagaaaa aacacgcatt attgtacagg gaatcaccgg aaaagaaggc
     1921 tcattccaca caggccaaat gcttgagtat aacacgagag ctaaagtggt cggaggagtt
     1981 actccgggaa aaggcggcca aaaatcggaa catggccttc ctattttcaa tagcgttaaa
     2041 gaatctgtag aaaaagaaca agcgaacgcc gccgtcatct ttgtaccccc accttttgcc
     2101 gccgacgcca ttttagaagg catttcagcc ggacttgctc ttgtcatttg cattaccgaa
     2161 ggcattcccg tgcgtgacat gtcgcgcatt gtagacttca tacgaaattc aaaaactgtt
     2221 gtcgtgggcc ccaactgccc aggggtaatc acccccggcg taggcaaaat gggcatcatg
     2281 cctggattta tccataaacc tggttcgatc ggtgtggttt cgcgttcggg taccctcacg
     2341 tacgaatcgg tgcatcaact tacgcaagtg ggtttggggc agtcgacgtg catcggtatt
     2401 ggcggagatc ccattattgg tttaccacat ttgcaggcag tgaaactcct cgccgaagac
     2461 cccgcgaccg agggcatcgt catgattggt gaaattggcg gcagcgcgga ggaagaggcg
     2521 gcagaatata tcaaggcgca tgtaaagaaa cctgtcgttg gttttatcgc cgggcaaacc
     2581 gctcctcctg gccggcgcat gggacacgcg ggtgcaatta tttccggttc tgctggcaca
     2641 gcacaaacca aaatgcaagc gatgaaagac gcaggcatcc atgtatgtga atcccccgca
     2701 caaatcggcg agacaatgaa aaaagcatta ggtaaataat cactcgcgca gcgcgctcca
     2761 aagcttgcgc catttcttta agatcgtttt gggcagacgn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnna ggcaagaccc tcacccccgc cgatatacaa ggcaagctgg tgctggtgtc
     2881 atttttcttt tcgcgctgct cgggtatttg ccccatggtg atggcgtgtg tgtggtgcat
     2941 ataccaacgt attaccgacc actcgaattt gatttttgtt tcggtgagcg tcgaccccga
     3001 acgcgaccgc aagctgcaac tcgccgagtt tcgcacgcgt atgctgggca aagacggcct
     3061 gccgggtaaa ctgccgttca agaactggta ttttacaacc ggcgataaaa ctgaaatcta
     3121 cgcactcgcg cgcggtgttt ttcaagccga cgggttgtgc gcgacccaaa agaaggcgaa
     3181 ccacgcgata tggcggattt tctacacact gagtcggttt atctattaga taaagattca
     3241 tacctacgcg gcatctaccg cacgcgtggc gataccgatt tagcacggtt ggtgaaagaa
     3301 attaagcaac tataatagag cttggcagtg tgactaaaag aacagtttaa gcatttatgt
     3361 ggatggcggt cgatgccatg agcggtgatt acgttgctgc ccctttagtg cagggcgcag
     3421 tggccgctac aggcctgttc tttagaagaa ggcagtaaga tacgtaaagt tatgccggtg
     3481 gattgatggg gacgatagca aaaccatttt tgaagtgggc gggcggtaag acgcagttgt
     3541 taccagagat tataaaacgt ttacccgcca atataaaaaa ccaaggattt acttatattg
     3601 aaccgtttgt tggtagcgga gccgttctat tttggatgct cggtcatttt gataacttgg
     3661 agcgcgcagt tattaacgac atcaatgaag atttagtcaa tacatataga gttgtagcgc
     3721 aagaaccgta cgagttaatt aagttacttc aaacttttca aattgaattt catcgtttag
     3781 aaaataacga cttagctaag aaaaaatact attatcagaa gagggagttg tataatagca
     3841 ggaggtcaga tacgattaaa caagccgccc ttttcatttt cttaaacaga acttgtttta
     3901 acgggcttta ccgcgtaaac aagcagaatg aatttaatgt gcctatgggt agctataaaa
     3961 aaccgactat ttgcgattct gaaaacctac tggcagttaa taaaattctt caaaaagtca
     4021 agattctttg cggtgatttt gaaaatacgt tggcatacgc acatgaaaac actctatttt
     4081 attgtgaccc cccgtacaaa ccgttaaatt taacatccag ttttaattct tatgcgaaag
     4141 atgagtttgg ggatgccgaa caaattaggc tcagcaactt ttgtaaaaga ctcgaagaac
     4201 ttaatcacgc ttggattctc agtaattctg atgtgtcggt cgtagaaagt aataattttt
     4261 tcaacgagct ttacggcaaa tttgaaatcc aaaaagttaa tgcaagacgt aatattaact
     4321 ctatttcaac caagagaggg gaagtcaaag aattgctcat cacaggcaat atggctttcg
     4381 caaactcatg atctttggcg gaaaaggcgg cggtaatact cgtacggggt tagtcttcga
     4441 agggaatacc gacttggcag attttttgaa taagcaaaaa aactataaag ttcaaaatgg
     4501 cgatgtcttg tataaagaag aaaaaatcgt acgcatcttc aaaaagtaca gcttctataa
     4561 attcttagat gaactgaaaa ttgactggtg cagccgaatt tcaaagaggc tgttacccga
     4621 cgatagtata ttcgtaatca ttgccaacac tctctttata attgaatgta aatttcaacg
     4681 cgtagccggt tctgtagatg aaaagttaca aacctgtgac tttaaaagaa agtaatacca
     4741 gaaattattg gcgcctgcaa atattgaagt agaatacata tacttactta gcgattggtt
     4801 tcgtaagcct gaatataaag acgtattaga ttatattcat agcgttcatt gctattattt
     4861 ttttgaatat ataccgctga ataaacttgg tctgccagtg ccagaatgaa aaatttacat
     4921 tacgaatctc aagatggtat gtttcgtctt tttcaaaatg actcgttaga agccttatca
     4981 actattaatg aaaaattcga tatggtcttt gtcgatccac cgtatttttt gtctaacaac
     5041 ggactttcta tccagagcgg cgagattgtg agcgtcaata aaggagcttg ggacaagtca
     5101 ccggggaatg ccgaaatcta tacgtttaac cgcgcatgga ttacggctgt gcgagagacg
     5161 atgaaaaacg atgctacgat ttggattagt ggcacgatgc acaatatttt ctctattggg
     5221 caaattttgc aagaacttag atttaaggta ttgaacgtga tcacatggga gaaaacaaac
     5281 ccaccgccga atttttcttg ccgctatttt acgtactcta cagagtttgt aatttgggca
     5341 agaaaaagcg ccaaagagaa gcattatttt aactatgacc tgatgaaaaa aatcgcaggt
     5401 gatcgccaaa tgaaagacgt ttggaaactc tctgcaattc agtcgtggga gaagacctgt
     5461 ggtaaacacc cgacacaaaa acctttggca ttattgactc gtattattct tgcttcgaca
     5521 aaacccaatg cacagatctt agaccctttt accggcagct caacaacagg tattgcggca
     5581 aatttggccg gtaggatttt tgtcggtatt gataaagaaa aagactttct tgaaataagc
     5641 cgccgtcgaa aaattgaaat cgaagacact cgcattgcac atctctaccg tgatagaatc
     5701 aacgattact atgcgctaag gtcttaaact tgccgcctgc aaaattgcct cttcgataaa
     5761 tgcccctcac aggctcctgc gagcctgtga ggctgtgcgc caacttggat agcgcgctct
     5821 tcttttcctt ctaaaaatta agaattcgtg aaatttagtg gctcacgcga ctgcggagcg
     5881 gactaatttg ccaaaattcg ttggcgtggc ggtgagttta acttctatga gtgctttttt
     5941 ttgccctttg gcgaatacct cttatgaaaa tttttcaaat ctaaagaatc aataagccga
     6001 gcaaagccaa acacttcatg atccgccttc agactttctg caatcgcttg agaatgtaaa
     6061 accatttgtt ctctgttggc gcctataaga cgattcatgt tacataatta aaagccacct
     6121 ctatttgcgc acgaaaattg atagttggcg cataggcgca caggagcctg tgcgtcaact
     6181 cactgagaca atttttttgc aaatttttgc tttgttaaac tgtgttattt ctcttcttta
     6241 cttattattt ttcgaagacc ataaaatacg gttttcgcgt tcgatttttt tctgcaaaag
     6301 tttggtgtcg ttttggcgca gcgcttgttg aatgcgttgg ctctcgatgg agttgagcga
     6361 gcggtatctt ttgagatgct ctttcatcga tttataaagg tcttcgccga tgaaatcacc
     6421 gtcgtcggcg agattgtcga tggtgcgcca aatatcgact gcgcggttgc cgttctgcca
     6481 cgaattgggg ttggcagggt cgatgctttc ccaaaagatg cggcctttcg cgagtgtcgc
     6541 gtctttagaa cggtagcccc ccggtgaagt ttcaacgaga tagtcgagaa tttctaaccg
     6601 cttcaagtag tagtgttgca atgcggcatc ttcgttgcgg atgcctttct tttctgccaa
     6661 aggcaaatag cgttttacca ctttttcaag agttttgatg cggatgttgt cgcgttcttt
     6721 gggggaaagt cggtcaatgt gttttttgac atcgaacaat aattttaacg cgcggccctg
     6781 gatttcataa atattttcaa tcgcgaataa aatctctgtg gcgtatttcg tccccttttt
     6841 tttagaaacc acgtcgagcg ctttgcgtaa aaactcttct ttgttaagcg cctcgtggaa
     6901 gtaatcaatc gcaaggtatg tcatgtcgct gttgaacgac gatgcaacgc gttgcaagtt
     6961 ttcataatac aaatcgcgga ttgcctgcgg gagatcgccc tcattgggat cgtaattacc
     7021 gtagtcgcgt aaaaaactat cgagctgttt ctctttagga tcagataaag tttcgtcaaa
     7081 atatttgcgc cgttctgctg cgttaaaatt gagtttgccg gcgctgcctt ttgcttggcg
     7141 gttgacttgc actttggtgc ggtttttatc ggcgatgcgc ttaccttcgc gccacaaacg
     7201 gtagttctcg ataatttcat tgcgctcttc cattttttcg agttcttgcg gcgcttcttt
     7261 gatttcctcg cggaagggtt tatcaatggt gtgcagcgtg ggaaaaatat ctnnnnnnnn
     7321 nnatgatgtt gcggtagtcg cgcttacgct tacggtactc ttcgggcaca tcgttgttga
     7381 tggcgccttt ctcgttgtct ttgtctgaat aactatcgtt taaggtctcg ccttcttcgg
     7441 tacggtcgat ttgctctttc catttgtcgc ctttgagacc aaatttttcg gcaaaatcac
     7501 cttcgccttt gccttgcgcg tcggccatgg ggccattatt aaaaccgccg ccgtcttctc
     7561 ctttttgatc gccttcttgc gagtgcggcg atttggggct gggggtgact tgcgccatga
     7621 gcgaaatttg gggctggtta caaatttcga tttcttgcgt ctttaagcct ttaatccaca
     7681 agtgggtaaa aagcgacaag tggagtattg cagataaata taaaaataac gaccaacgct
     7741 gccggcccaa aaaatgcaag aagctgtctt ttaaaaaggc ggccatgggt tgcgtttcgt
     7801 ttctgcccca gccgtgtcaa ggaatcgtat agatggggtc gcactcactg cccgatgggg
     7861 tatccacaca atccaagtta atgcgtgaca cgcggccatt atataccgac gtcataaaga
     7921 gataattgtt gcgagtgcct ttttgactcg ctttcacgag taccaatcca tgcggctgtg
     7981 cataataatc ggagggatag taactgccaa cataaggatc gtcggcaggg ttggtcgagg
     8041 gggcggaagc tgtcgtctcc caacctacac gaccatggcc gatccaaccg cagaacttcc
     8101 ctgagcttat aggcttatcg cagttgaggc cgtcgatccg ccatttcacg atgcggttgt
     8161 tgccgcgatc ggcgacataa aggtatgtgc catcggttac caccgcggaa ggataacgaa
     8221 agtatttcac cgccgagtta cccgtttggt taggcgcggt tgtcataact tgccaatcgt
     8281 cgtagccgct acccaaccag cgttcaaaat tgccgctagc ggcatcgatg cgcacaatac
     8341 gctggttccc ttcgtcgaca acatagagtt ttccgttggc gattgcgaca ccacgaggac
     8401 caatcgaacc ataccccaaa taacccgccg tgtgaccata cgcaggggat tgtgttgtcg
     8461 ctgtagtatt ccacgcattt tcaccattcc cgataaaacc accatacgcg cctgtgaggc
     8521 ggttgcggcg actaatacgt ttaccgccat aatcgaccac ataaagatac gtttggctgg
     8581 catcgtcggg attg
//
LOCUS       DAOMPD010000044         8584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3540_length_8584_cov_2.527377, whole genome shotgun sequence.
ACCESSION   DAOMPD010000044 DAOMPD010000000
VERSION     DAOMPD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_8584_cov_2.527377"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..576)
                     /locus_tag="PLY93_02550"
     CDS             complement(151..576)
                     /locus_tag="PLY93_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRP68391.1"
                     /translation="MFFFIFVLLIVNEEPPWAARWTFIQAKAKQPEIGKLLDDAMDAI
                     EKDNTSLKGALPKNYARPALDKPRLGELIDLKADYVLANPPFNDSGWGGEHLREDVRW
                     KYGASPTGNVNFAWVQQKTIDFAGGFRYKPTTSAIVRVV"
     gene            complement(729..812)
                     /locus_tag="PLY93_02555"
     tRNA            complement(729..812)
                     /locus_tag="PLY93_02555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Leu,seq:cag)
     gene            896..1471
                     /locus_tag="PLY93_02560"
     CDS             896..1471
                     /locus_tag="PLY93_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68392.1"
                     /translation="MEAFQQDKYFAKMKFWRLWGNVIRVFDASNTKLLLYVKQKAFKL
                     KEEITIFADETQNTPVLKIKARSIIDFGATYDVADAVTGEKIGALRRKGLKSILKDSW
                     LILDASDNEIGKVEEGSVIMAMLSRTINFIPQHYQITMGDKLVGKIDQTWNIFMPQMR
                     IDFSMDTAKVLDRRLGIATVVLLQVIEGKQQ"
     gene            1592..1822
                     /locus_tag="PLY93_02565"
     CDS             1592..1822
                     /locus_tag="PLY93_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68393.1"
                     /translation="MRKPSIAASRNKFKKVLSQYVKHQGLDLEVTLKNGEKVTLSKMR
                     KIEGDHVVSRTARGAESLLAIRDIQHLEVFVS"
     gene            complement(1839..2585)
                     /locus_tag="PLY93_02570"
     CDS             complement(1839..2585)
                     /locus_tag="PLY93_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP68394.1"
                     /translation="MKTSVFFLTTILSVHLYAETSNDYRTQRAFEAGEDSESRQENAI
                     RECAVSQIQKCVYPIIAQLKKEGKENAPLRRESANALGRMHATEAREPLLQMLSKEDD
                     EFAKGAAIGALGLIANKEDIKTVAPYTTDKSRYIRRRTARALYDFDNKEASAEAANKA
                     AAEKDDLTRAELLNAALRYESGKVEHVLALTKILLSPDRAARLRAAEILGDYQNKEAL
                     ADLRRAHEIELDSGVRAALYRAIQTTLMGY"
     gene            2701..4047
                     /locus_tag="PLY93_02575"
     CDS             2701..4047
                     /locus_tag="PLY93_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HRP68395.1"
                     /translation="MLQKTVENFGFSDAEKNFRNAFIEKNRGKPLFLMLVENLHEMHI
                     DEFVKNIPEQIKRVNDEEYQSVQMYVLGDEARFYVGIVPSDDARGFLPNIDGAMGKLH
                     GEIGKGKAGDFDFGIGRTQCNYISDSQEIFEELIKAAHKNLEDNLVRWSWTYLNRAND
                     YFANIHANAVIQPIIYFDPKKELYSVKGGEVFVGGDLYSGYAELLSDIPKDQDINRIE
                     LLILEKIIMACHDAPGLLKFNVSPQTLIDTFPSAEKVFRFNELLLKQKLNPHNVRLEL
                     VEQPYEEKEIKLKDVCRYFFQFGISFAADDFGVKSQSHQVILDLGEMIKEFKLDPISF
                     KFKPEEDQTKFLDNLAFIDSCIRWADNREAIITAEAVDDYDTMRFLIAHQVFYFQSHI
                     FCKKMSLQYYKENFHRMQNLPESAVRKILSEDDLISRQKEEGNIFTLTDTLDLWEK"
     gene            4052..4609
                     /locus_tag="PLY93_02580"
     CDS             4052..4609
                     /locus_tag="PLY93_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68396.1"
                     /translation="MKKIILYFAVAFLFTLSAEGVAQTKKFADRGIFEIGGRLDFSYL
                     DKERGAGLGHETQGNFSFAPQLGYFLTANFEVSVLPTVNVNFYDNRATETLYGAMLAP
                     AYVLPYLRPFYPYIEGLVGSTLSKNNQTVSWGVGTGIKILVLENALLKLGFTYLNNNK
                     TSRVDSSIYEKENLLNFSVGFGIFF"
     gene            complement(4625..4996)
                     /locus_tag="PLY93_02585"
     CDS             complement(4625..4996)
                     /locus_tag="PLY93_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68397.1"
                     /translation="MRHFFIATLALLPFLTGCILTEKRATIRITDASGYADMCVKIEG
                     RQKTEAQSLPYTYTAEEGETVSLKAFISTNSTVIATGSITTRMTNDCENEVIYYAEIR
                     NGYFNITDDYVPVSDCKSTSK"
     gene            complement(5002..5403)
                     /locus_tag="PLY93_02590"
     CDS             complement(5002..5403)
                     /locus_tag="PLY93_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68398.1"
                     /translation="MRRAIAVFSVFVFLAGPIYSECNPPASILATCSSSTPNSSSSSN
                     SSISFDPKISSSLLIIGVTVLIGIALFYYLKDSLFKSSSAVAYHESTLYKSFNNLPAA
                     RDAFAVTRERLAHPIEVGFQNWNFNLPGLKW"
     gene            5486..6637
                     /gene="carA"
                     /locus_tag="PLY93_02595"
     CDS             5486..6637
                     /gene="carA"
                     /locus_tag="PLY93_02595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRP68399.1"
                     /translation="MAVMHDGVLVFADGTYFKGTLFGAPTTADKSLGEAVFQTAMSGY
                     QESLTDPSCRGQVVCFTYPSFGNYGVNSRDMESKKIWLSGVVVRDLCEIPSNFSATMT
                     VDEFLKKENTTGLSGVDTRAVVRKIREGGAQMAGIFSFSEETNIAALAQKVKAQPSMD
                     GQNLVADFDGVDANAFVKSYIAENKISVEGLIPLAVLDFGIKYNILRELIRAGFNPTV
                     FAGDTPLNAQKNFNIDGYRGFFFSNGPGDPAVVQNGIANIRQLMDSGKPCLGICLGHQ
                     MLALAMGANTYKLKFGHHGGNQPVKTDYRKQVIITSQNHGFAVDEASLKKATAKEACF
                     EVNANDLSAEGFRVINDTHKIMSVQYHPEAAPGPRDASIVFAEFHALFR"
     gene            6686..7486
                     /locus_tag="PLY93_02600"
     CDS             6686..7486
                     /locus_tag="PLY93_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68400.1"
                     /translation="MPLSEAQKTELLTLQKLARQYEGIIRTSRNADQQGKAKIELKKI
                     RDKIEAIAPGGAADEFLAANPQQTGGYGRKKELEDLTSTYPTLAKMPMARITPNSDEL
                     EINILGSVLMAWESTFYPALGKIKLDFALSAERDSHFTVLESLKRQMKILIETIEDVQ
                     RAVREDSKAQLRDMRLRQQGQFMFDGGSFLKKVYEFWKAVYEDIQANGPKCFNKDDSI
                     IVDTRLEVANYFHGKSVRETARFTVMFLHEAIEALNLPDLPVDKNKQR"
     gene            7492..>8584
                     /locus_tag="PLY93_02605"
     CDS             7492..>8584
                     /locus_tag="PLY93_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP68401.1"
                     /translation="MAAKKQNTNAVQKKELSKVDTPRTQSATDIIQFGTAWEYAESIE
                     SSSIVKIAPKYNLFINGKFTPPQSGKYFATINPATEQKIAEVAEANDADVDAAVRAAR
                     AAYDKVWSKLPGKERAKYIYRIARLIQDRARELAVLESIDGGKPIRESRDVDVPTAAA
                     HFFYYAGWADKLDYAFPGRKLKPLGVAGQVIPWNFPILMAAWKLAPALACGNTVVLKP
                     AETTPLTALFLAQILQEAELPDGVVNIVTGAGATGAAVVAHPDVNKVAFTGSTAVGKK
                     IQQALAGSGKKMTMELGGKAANIVFEDAAIDQAIEGIVNGIYFNQGHVCCAGSRLLVQ
                     ESIHDTVVKKLSRRLQTLRVGNPLDKNTDI"
BASE COUNT         2366 a         1931 c         2068 g         2219 t
ORIGIN      
        1 tcaagccaaa acaattgaag cgcgttcaag cagtgcttgc ccgaagtgat ggcctgaaag
       61 tagcccaggt cgtattaagc cagttagctc tgcgctggag gaaaaagttt gcaggctgac
      121 gatccaggag aagccaaaga atgcaacgca ctaaactaca cgcacgatgg ctgatgttgt
      181 tggtttatac ctgaatccgc ctgcaaaatc aatggttttc tgctgcaccc aggcaaagtt
      241 cacattgccg gtcggtgacg ctccgtactt ccaacgcacg tcttcgcgca aatgctctcc
      301 accccaaccc gaatcgttga atggcggatt tgccaaaaca tagtcggctt tgaggtctat
      361 cagctcgccg agccgcggct tatcgagcgc ggggcgggca tagttcttcg gtagcgcacc
      421 tttcagcgac gtattgtcct tttcgatggc atccatcgcg tcatcgagca acttgccgat
      481 ttccggttgt ttggctttgg cctgaatgaa agtccaacgt gccgcccagg gtggttcttc
      541 atttacaatt aggaggacaa agatgaaaaa gaacacaaac aaggttgatg actacgagca
      601 agttacttcg agagtcattg cgccactttg ctctggatga tctatgaccg aaggagacag
      661 cccgcaagca tagtcccgcc tataagttag atgagaactg ggatttgcga ggaatgtccc
      721 atcatttttg cccaaagggg gacttgaacc cccacggggt tgcccccact agtacctgaa
      781 actagcgcgt ctaccaattc cgccatctgg gctattatgc gttgggagtt tttaagattc
      841 gcgacacgcc gtcaattttg attgatatgt acactggtgt tttgaatgag tcccaatgga
      901 agcatttcaa caagataaat attttgccaa aatgaagttt tggcgccttt ggggtaacgt
      961 cattcgagtt tttgacgcct cgaataccaa gttattgctt tacgtaaaac aaaaagcttt
     1021 caagcttaaa gaagaaatca cgatttttgc tgatgaaacg caaaacactc cggttcttaa
     1081 aattaaagcg cgcagtataa tcgatttcgg cgcgacgtac gatgtggcag atgcagttac
     1141 aggagaaaaa atcggtgcat tacgccgcaa aggtctcaag tcgattctta aagattcgtg
     1201 gcttatttta gatgcaagcg ataacgaaat tggtaaggtt gaagaaggta gtgtaattat
     1261 ggcgatgctg agccgtacta ttaactttat tccacaacac tatcaaataa cgatggggga
     1321 taaacttgta gggaaaatag atcaaacttg gaatattttt atgccacaaa tgcgcatcga
     1381 tttttcgatg gatactgcca aggtgctcga ccggcgattg ggaattgcaa cggtagttct
     1441 tttacaagtt atcgaaggta agcaacaata aatcaatttc tcttcagtaa ttcttaagac
     1501 tgccgatact cattatgtca catagtggta ttcttcaagg atgaagaatt atgagcggca
     1561 tggatgccaa taaaaaaatt aaggaacatc catgcgcaaa ccatcgattg cggctagccg
     1621 caacaaattc aaaaaagtac tttcacaata tgttaaacac caaggcctcg atttagaagt
     1681 gacactcaaa aatggcgaga aagttacatt gtcaaaaatg cgaaaaattg aaggcgatca
     1741 cgttgtttca cgcaccgcac gtggggcaga atcgttgctg gcgatccgcg acatccagca
     1801 tttggaagtt tttgttagtt aataacgcat gttattaact aataccccat gagtgtcgtt
     1861 tggatagcgc ggtaaagcgc tgcacggaca ccgctgtcga gttcaatttc atgtgcgcgg
     1921 cgtagatcgg ccaacgcttc tttattttga tagtcgccta aaatttcagc ggcgcgtaat
     1981 cgggcggcac gatcggggct gagaagaatt tttgttaacg ctaagacgtg ttctactttt
     2041 ccgctttcat agcgcagcgc cgcatttaag agctctgcgc gcgtcaaatc gtctttttct
     2101 gcggcggctt tattcgccgc ttcggcggac gcttctttat tgtcaaaatc gtagagcgcg
     2161 cgtgcggtac gacggcggat ataacgactt ttatcggtgg tataaggcgc aaccgttttg
     2221 atgtcttctt tgttggcgat aagtcccagc gctccaatcg ccgcaccttt ggcaaattca
     2281 tcgtcttctt tagatagcat ttgtaaaagc ggttcgcgcg cttcggtggc gtgcatgcgc
     2341 cccaatgcgt tggcgctttc acgccgcagc ggcgcatttt ctttcccttc cttctttaac
     2401 tgtgcaatga tgggatagac acacttctga atttggctca ctgcgcattc gcggatagcg
     2461 ttctcttgcc gactttcgct atcttctccc gcctcaaaag cgcgttgggt gcggtagtcg
     2521 ttactggttt cagcgtagag gtgaaccgag aggatagtgg ttaagaaaaa tacactcgtc
     2581 ttcataggat taccagcaca attttcacgg cgcgccgtca acggtaatat ttcccgtaga
     2641 actattttca tttacggcaa acctcgcttc acaacctaag cacccgtggc gttccgagcg
     2701 atattacaaa aaaccgttga gaattttggc tttagcgacg ccgagaaaaa tttccgtaat
     2761 gcattcatcg agaaaaacag aggcaaaccg ttgtttttaa tgctcgtgga aaatttgcac
     2821 gaaatgcaca tcgatgaatt tgtgaagaat atccccgagc aaattaaacg ggtcaacgac
     2881 gaggaatacc aatcggtgca aatgtatgtg ctgggcgatg aagcgcgctt ttacgtgggt
     2941 atcgtaccgt ccgatgacgc gcgcggtttc ttgcccaaca ttgatggtgc gatgggaaag
     3001 ctgcatggcg aaatcggcaa ggggaaagcc ggggattttg attttggcat cggtcgcacg
     3061 cagtgtaatt atatttcaga ctcgcaagaa atttttgaag agttgattaa agcggcgcac
     3121 aagaacctcg aagataatct tgtccgatgg agttggacat acctcaaccg cgcgaacgat
     3181 tattttgcga acatccacgc caacgctgtg attcaaccca tcatttattt tgatcccaaa
     3241 aaagaattgt attctgtcaa aggcggtgag gtctttgtcg gcggcgatct gtattcgggg
     3301 tacgccgaac ttttaagcga catcccaaaa gaccaagaca tcaatcgcat tgaactttta
     3361 attttagaga aaattattat ggcgtgccac gatgcgccgg ggcttttgaa attcaacgtt
     3421 tcaccgcaaa cgctcatcga tactttcccc tcggcggaaa aagtatttcg ttttaacgag
     3481 ttactcctca aacaaaaact caatccacat aacgtgcgct tagagttggt cgagcaacct
     3541 tacgaagaaa aagaaattaa actcaaagac gtgtgccgct attttttcca gtttggcatc
     3601 tcttttgccg ccgacgattt tggagtgaaa agtcaaagcc accaggtgat tctcgatttg
     3661 ggcgaaatga ttaaggagtt taagctcgac ccgattagct tcaagtttaa accagaagaa
     3721 gatcaaacta aatttttaga caacttggcg ttcattgatt cttgcatccg ttgggccgac
     3781 aaccgtgagg caatcatcac cgccgaggcc gtggacgatt acgacacgat gcgctttttg
     3841 attgcacacc aggtgtttta ttttcaaagt catatttttt gtaaaaaaat gtcgttacaa
     3901 tactacaaag aaaacttcca ccgcatgcaa aatttgcccg agagcgcggt gcgtaaaatt
     3961 ctttccgagg acgatttaat ttctcgtcaa aaagaagaag gcaatatttt caccttaacg
     4021 gacactttgg atttgtggga gaaataagtt tatgaaaaag ataattttat attttgccgt
     4081 tgcgtttctt tttacgctat cggctgaggg cgtggcgcaa actaagaaat ttgccgaccg
     4141 tggtatcttt gaaattggcg ggcggctcga ttttagttat ttggataaag aacgcggcgc
     4201 gggtttaggc catgaaacac aagggaattt tagctttgcg ccgcagttgg gctattttct
     4261 taccgccaac tttgaggtga gcgttttacc aacggtgaac gttaattttt acgataaccg
     4321 cgccaccgag actttatacg gcgcgatgct tgcacctgcg tatgtgttgc cgtatttacg
     4381 tccattttat ccttatattg aagggttggt aggaagtacg ctcagcaaaa ataaccaaac
     4441 cgtgagttgg ggcgtcggaa ccgggattaa aattctcgtc ctggaaaatg cgttgttaaa
     4501 attagggttt acctatctca acaacaacaa aacgtcgcgc gtcgattcga gtatttatga
     4561 aaaagaaaat ttattaaatt tttctgtcgg ttttggaatt tttttctaaa gcggatataa
     4621 agcgctactt ggaagttgac ttacaatcgc ttactggtac atagtcgtcg gtaatgttga
     4681 aatagccgtt gcgaatctcg gcgtaataaa tcacttcgtt ttcacagtcg ttcgtcatgc
     4741 gcgtggtgat cgatcccgtt gcaatgacag tggagtttgt ggaaataaat gccttgagcg
     4801 acaccgtctc tccttcctcg gccgtgtacg tgtatggtaa cgactgcgct tcggtttttt
     4861 gccgcccttc gattttaacg cacatatcgg cgtatccact ggcatcggtg atgcggatgg
     4921 tcgcgcgttt ttcggttaag atacaaccgg ttaaaaacgg caacagagca agtgtggcta
     4981 taaaaaaatg acgcatgtga gttaccattt tagccccggt aaattaaaat tccaattttg
     5041 aaatcccact tcgattgggt gtgcaaggcg ttcgcgtgta acggcaaagg catcgcgcgc
     5101 tgcaggaaga ttattaaacg atttatataa agtggattcg tgataagcaa cggcagagct
     5161 acttttgaat aaactatctt tgaggtaata aaaaagtgcg atgccaataa gaacggtgac
     5221 gccgataatc aggagcgacg aacttatttt agggtcgaat gaaatcgaac tgttggacga
     5281 tgaactggaa ttgggagtag agctggaaca cgtcgctaaa atcgaagcgg ggggattgca
     5341 ttcgctataa atgggtcctg ctaaaaaaac aaatactgaa aagacggcga tggcgcggcg
     5401 cataccctat tttcccattg ttagtcggta tgccaactat aataaagctt tttacgacgt
     5461 atcgaaaatt ttaggtgtgt caactatggc tgtcatgcac gatggggttt tagtttttgc
     5521 ggatggtact tatttcaaag gcacactttt cggcgcaccc accaccgccg ataaaagttt
     5581 gggtgaagcg gtgtttcaaa ccgcgatgtc gggttatcaa gaaagcctca ccgaccccag
     5641 ttgccgtgga caagtggttt gtttcactta tccttcgttc ggcaattatg gcgttaatag
     5701 ccgcgacatg gagtctaaaa aaatttggct ctccggtgtc gttgtgcgcg acctgtgtga
     5761 aatccctagc aactttagcg cgacgatgac cgtcgatgaa tttttaaaaa aagaaaacac
     5821 cacaggctta agcggtgtgg atacacgcgc ggtggtgcgt aaaatccgcg aaggcggagc
     5881 gcagatggcg ggaatttttt ctttctcaga agaaacgaat atcgccgcgc tcgctcaaaa
     5941 agtaaaagcc cagccttcga tggacggtca aaatttggtt gccgattttg atggcgtcga
     6001 tgcaaacgcg tttgtgaaat cgtatattgc cgaaaataaa atttccgttg aagggttaat
     6061 cccactggcg gtgctcgatt ttggcatcaa gtacaatatt ttacgcgaac ttattcgcgc
     6121 aggttttaat cccactgttt ttgcgggaga cactccttta aacgcgcaaa aaaatttcaa
     6181 catcgacggc taccgtggtt ttttcttctc gaatggccca ggcgatcccg cggttgttca
     6241 aaacggcatc gccaatatcc gacaacttat ggattcggga aaaccctgcc tgggcatttg
     6301 cttggggcat caaatgctgg cgttggcgat gggcgccaat acatacaaac tcaaatttgg
     6361 ccaccacggc ggcaatcaac ctgtgaagac cgattaccgc aaacaagtta tcatcacttc
     6421 gcaaaaccac ggtttcgctg tcgacgaagc gtcgctcaag aaagccactg cgaaggaggc
     6481 gtgcttcgag gtaaacgcaa acgacttgag tgcagagggg tttcgcgtta tcaatgacac
     6541 gcataaaatt atgagcgtgc agtaccaccc agaagccgca ccagggccgc gcgatgcgtc
     6601 gattgtcttt gctgaattcc acgcgctttt tcgttagaag tcttcttttt gctttacact
     6661 cgcaccgccc gccgcgtcgt accttatgcc attaagtgaa gcccagaaga ctgaactttt
     6721 gaccttacaa aaacttgcaa ggcaatacga agggattatc cgtacatcgc gtaacgccga
     6781 tcaacaaggc aaagcaaaaa ttgaacttaa aaaaatccgc gataaaattg aagcgattgc
     6841 tccgggagga gcggcggatg aatttttagc ggcaaaccct caacaaacgg gcggctatgg
     6901 ccgtaaaaaa gaactcgaag atttgacatc cacgtaccca acgctcgcca aaatgccgat
     6961 ggcgcgcatt acacccaact cggacgaact ggaaatcaat attttaggca gcgtgcttat
     7021 ggcatgggaa agcacattct atcctgcatt gggtaaaatt aaactcgact ttgctctttc
     7081 tgcagaacgc gattcacact ttactgtgct tgaaagttta aagcggcaaa tgaaaatcct
     7141 catcgaaacg attgaagacg tgcagcgtgc ggtgcgcgaa gattccaaag cacagctccg
     7201 cgatatgcgt ttgcggcagc aggggcaatt catgttcgac ggcggttcgt ttttgaaaaa
     7261 agtatacgaa ttctggaaag cagtttacga agacattcaa gccaacgggc ccaaatgttt
     7321 caacaaagac gatagcatta tcgtcgatac ccgcctcgaa gttgccaact atttccacgg
     7381 taagtcggtg cgcgaaacgg cgcgttttac agtcatgttt ttacacgaag caatcgaggc
     7441 gttaaattta cccgatttac cggtagacaa aaataaacag aggtaacacc tatggcagca
     7501 aagaaacaaa atacaaacgc agtacaaaaa aaagaactct ccaaggtaga taccccacgt
     7561 acccaaagcg caaccgatat tattcaattt ggtacagctt gggaatatgc ggaaagtatc
     7621 gaatcgtcgt cgattgtaaa aattgctcct aaatacaatc tttttattaa cggtaaattt
     7681 actcctcccc aaagcggcaa atatttcgcc actataaacc ccgctaccga gcaaaaaatt
     7741 gccgaggtcg cggaagcaaa cgacgccgat gtcgatgcag cggtgcgtgc ggcacgcgcc
     7801 gcgtacgata aagtgtggtc aaaactgccg ggtaaagaac gtgcaaaata tatttaccgt
     7861 attgcacgcc tcattcaaga ccgtgcacgg gaactggcgg tgctcgaatc catcgacggc
     7921 ggtaaaccca ttcgcgaatc gcgagacgtg gatgtaccga cggcggcagc gcactttttt
     7981 tattacgccg gttgggcaga taaactcgac tatgcatttc ccgggcgtaa attaaagccg
     8041 ttgggagttg caggacaggt tatcccttgg aattttccga tacttatggc ggcgtggaag
     8101 cttgcaccgg cgctggcatg cggtaacacc gtcgtcttaa agcctgcgga aactactccg
     8161 ctcaccgcac tttttctcgc acaaatttta caagaagctg aactccccga tggcgtagta
     8221 aatatcgtca ccggcgcggg cgcaacggga gcggcggttg ttgcgcaccc cgatgtcaac
     8281 aaagtagcgt tcaccggatc gaccgctgtg ggtaagaaaa ttcagcaagc actcgccggt
     8341 tcgggtaaaa aaatgacgat ggagcttggc ggtaaagcgg cgaacattgt ttttgaagat
     8401 gctgccatcg accaagcgat cgaaggaatt gtcaacggga tttatttcaa ccaaggccat
     8461 gtctgctgcg ccggttcgcg gcttttagtc caagagtcca tccacgacac cgtggtgaaa
     8521 aaactgtcac ggcgcttaca aacgttgcgc gtgggtaatc cgctcgacaa aaacaccgac
     8581 attg
//
LOCUS       DAOMPD010000045         8478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3587_length_8478_cov_2.797103, whole genome shotgun sequence.
ACCESSION   DAOMPD010000045 DAOMPD010000000
VERSION     DAOMPD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3587_length_8478_cov_2.797103"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="deoC"
                     /locus_tag="PLY93_02610"
     CDS             complement(<1..615)
                     /gene="deoC"
                     /locus_tag="PLY93_02610"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRP68402.1"
                     /translation="MKSLASTAMRMYPSPKLDPMYIPVDQVAVEERVARFKTRSIKND
                     AKLAGIRLAISMIDLTTLEGKDSPGKVIQLCQKALRPSELPGVPQVAAVCVYPNMIPI
                     AKKALKGSKIHIACVSTAFPAAQVPLEIKLEDVRRSVDLGADEVDMVISRGQFLAGEY
                     NYVFDEIAAVKEACGKAHLKVILETGELETLDNVRRASFIVMRAG"
     gene            704..>1100
                     /locus_tag="PLY93_02615"
     CDS             704..>1100
                     /locus_tag="PLY93_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HRP68403.1"
                     /translation="MSSTLSFNTLRTSLFAEHQNLKAKFTPFAGYNMPVSYGAVKDEV
                     SAVRTACGLFDVSHMLPISLKAASRDALVEALSRVCVRVPNKLKVGRAQYNALYNENA
                     GLTDDVTLSALSDTHWLMVANAGNRQADVA"
     assembly_gap    1101..1118
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1119..1798
                     /locus_tag="PLY93_02620"
     CDS             <1119..1798
                     /locus_tag="PLY93_02620"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRP68404.1"
                     /translation="ISDTVKIEPWDNYTLLAVQGPDAPKLLEAKFPEIKNLYYYEVAF
                     RGEDTFIARMGYTGEDGFEIACPQAEGIALWKELIAAGAKPCGLAARDVLRLEALMPL
                     YGHELSSTLRPQESGLGFLYSDHTSIAPAALRNQATAQNSFGFQMQGEGIPRQGYKVV
                     RESGEVVGEVTSGTFSFTQNCGMGFMRIDIAQKDKALFVEIRGEKKPIAVLEKTPIKG
                     SVRRRPK"
     gene            1776..2687
                     /locus_tag="PLY93_02625"
     CDS             1776..2687
                     /locus_tag="PLY93_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68405.1"
                     /translation="MSVDDLNKNIEARTATPQKRAQELIREFVEFSEKYSAQIPLAIP
                     KGTLKTIDHYRDTYFKYLKDSEFKSNICYMLQLIEYQLWQYKLFKPQYSLENALFFQL
                     LITQGIVIEAVVFALIADPLVTSDKLDRSKGSTNDEYSLLLNLIRRRTFSSHVQQLAA
                     MEILSEPLREKLDTFRSEIRNLVHLQNWDGRLYRQITLVEYTKHLHEFDVLLKELNAQ
                     VTAVHTLDVLLQYYQLDGRSIKGIVKFYNRNSRRGQIDAKSVHDYIPFFYEDSFGDWL
                     PQKNDVVFFTLALNEHGVRAIQVKPNR"
     gene            complement(2689..3660)
                     /locus_tag="PLY93_02630"
     CDS             complement(2689..3660)
                     /locus_tag="PLY93_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP68406.1"
                     /translation="MAKQSTADRERDQIVLELIQGIKKGDSDALAAFFEIYSDDIYNF
                     PIRLFRFTEDEASEFYLYAFEHLRDGRKITSFQGKAKFTTWFYAVLRNLTIDFLRTQK
                     DKKNFVHNRKFDGTGKLLDAVDADVGTREEGLAEGSLFHKFSESLAAQPINQRVLFKL
                     AYAWYFELTVDEIEYLKDLKKTSEVEILKRVQKLKGIALERSNEVRDYEDKLTSNFQG
                     ISLIESKINAFFRENPEIERKREMWHENFFPQTMPSQIIDLIQSLMKRKKRQESLLTQ
                     QKRSLLSIRVPYKDLGEILNSTQGVLSVQLVRIVEKLSQSIGASFTD"
     gene            complement(3732..5561)
                     /gene="glmS"
                     /locus_tag="PLY93_02635"
     CDS             complement(3732..5561)
                     /gene="glmS"
                     /locus_tag="PLY93_02635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRP68407.1"
                     /translation="MCGIVGYIGPKSVESVLLVGLQKLEYRGYDSAGMAVLDGDEITT
                     RKAVGKIAELQKKLKSQPMFGSVGIGHSRWATHGEPSTVNAHPHINKDSTIAVVHNGI
                     FENYAKLRAELADDGYVFVSETDTEVAPHLMDKYLEEGFSPDEAFARTLRRLEGQYAL
                     AVISEKAHRKIYFARKGSPLIIARGNNEYILASDIPAVVPICDEAIYLENGEWGYITP
                     EGITIFNAAGDPVTPKWETVSVSVEDVDKGEFDYLMLKEIHEQPDVFERIFKERLDDT
                     FIHFPELKFGKEYLAQVGRILIQACGTSLNAGMIGRMYIEQFARISTETDYSSEWRYR
                     NPLTSGDSLVMGISQSGETADTLAGLREAKAKFMRVLSFLNNVNSTMARESDALVNLM
                     AGPEIGVASTKAFTAQVLNLFLFSLYVGSIKWLIPSEERAKLLSELRSIPDMMRKMLK
                     NHDSIKEMAKFLKDKNDTIFLGRTYNYPSALEGALKLKEISYIHASGYAGGEFKHGPL
                     ALISDNVPVVIIAPQGEIRTKMISNLKEAQSRKGKIISIITEGDDEIKKESDFYFEIP
                     ACDESISPLLVAIPLQLLAYYTAIERGCDVDRPRNLAKAVTVE"
     gene            5613..6119
                     /gene="tpx"
                     /locus_tag="PLY93_02640"
     CDS             5613..6119
                     /gene="tpx"
                     /locus_tag="PLY93_02640"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRP68408.1"
                     /translation="MAQVTFHEKPVQTAGNLPAVGSKIPDFRLTKQDLSDLKSADLIG
                     KKLVFNIFPSIDTPTCATSVRKFNAQAASLPNTVIVCVSSDLPFAQKRFCGAEGLTNV
                     VTASDFKDKKFGRDWGVYFTDGPLQGLMSRAVVVADATGKVVYTEQGAEVVNEPNYDK
                     ALAAVKSA"
     gene            complement(6292..6636)
                     /locus_tag="PLY93_02645"
     CDS             complement(6292..6636)
                     /locus_tag="PLY93_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68409.1"
                     /translation="MTTTLQMIFQLSAPARRALAAIASPQQLELMAERFYDTLQHERY
                     RDFSVTPTYYTTAHGHRKAQSLKQVEQREAYAAVLTLKTLQRMKKLTERGLSITWLME
                     EILYFGFCRGMI"
     gene            7009..>7391
                     /locus_tag="PLY93_02650"
     CDS             7009..>7391
                     /locus_tag="PLY93_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68410.1"
                     /translation="MPLLRKYVLFACCYLFIGVAVLYTHYLQTSPYQKDALDEKLTLV
                     AASHFSLPQLVDNLPTVFRENSHAAALKLTNFKGEFLGAMYDARRMRSDEYRQFLDTK
                     SFTVQKSPLIGYEILIWESKGRKLR"
     assembly_gap    7392..7440
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7447..>8478
                     /locus_tag="PLY93_02655"
     CDS             7447..>8478
                     /locus_tag="PLY93_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68411.1"
                     /translation="MSNTYALHYLIPIYVLFGGLLLFMYHWFWGSESHSRHLFSEKKK
                     RLPFFAPAKTTVDSAAALSPTEPKHRWQLKSGVIAEGGIHATLAQLRSLAGAESVRLF
                     AKLNNQWLPVAEVRGQLTVRGEAMELPSLLTQPFASTEEFLFDTVYGECLAFNKAPQN
                     AELCFVLKLNSTKEIAESEMRERIGTFVKTHSRSLLVEHYYESSILDAESGLYSFPYA
                     MFTLKEKILGGLTFSAAVFQFPESFFTSSHLSKIARTAIRAMREYFAADEAPVIARGS
                     GNRLFIIFNARREGAKDYAEEATLKLFTSYENIFKTEFQLQAGFVADAAGFGSSTRAI
                     KILERLLDES"
BASE COUNT         2120 a         2002 c         2016 g         2273 t
ORIGIN      
        1 gcccgcgcgc atgacgatga agctcgcgcg gcgcacgttg tctaacgttt cgagttcgcc
       61 cgtttcaaga ataactttga ggtgtgcttt gccgcacgct tctttcaccg ctgcaatttc
      121 atcgaacaca tagttatact caccggcaag aaattgaccg cgcgagataa ccatatcgac
      181 ttcgtcggcg cctaaatcga ccgagcgtcg cacatcttcg agtttaattt cgagaggcac
      241 ttgcgccgca gggaaagccg tcgacacaca agcgatgtgg atcttggaac ctttgagggc
      301 ttttttagcg atgggaatca tgttggggta cacacacacc gccgcgactt gtggaactcc
      361 aggaagctcc gagggacgca gcgctttctg gcagagttgg atgactttac ctgggctatc
      421 tttgccttcg agagtggtta agtcaatcat gctgatggcg aggcgaatcc ccgcgagctt
      481 tgcatcgttt ttgatgctgc gtgttttaaa acgagcgacg cgttcttcta ccgcgacttg
      541 atcgacgggg atatacatcg gatcgagttt ggggctggga tacatccgca tcgctgtgct
      601 cgcaagtgac ttcatggtgt tgagtcggtg cgccgcgagg ggcgtcaagt agaaagggaa
      661 ggttcctcag tgtacggcgt gtaactcttt tctattttta cctatgagta gtacactatc
      721 cttcaatacg ctcaggacat cgctctttgc agagcatcaa aatttgaaag ccaagtttac
      781 accatttgca ggctacaata tgcccgtctc ttatggcgcg gtgaaagacg aagtgagtgc
      841 agtgcgtacg gcgtgcggtc tttttgacgt gtcgcacatg ctgcccattt cacttaaagc
      901 cgcctcgcgc gatgcactcg tcgaggcatt gtcgcgcgtg tgcgtgcgtg tacccaataa
      961 actcaaagtg gggcgcgcgc agtataacgc tctctacaat gaaaacgccg gtttaaccga
     1021 cgatgtgacg ctctcggcat taagcgatac gcactggctc atggtggcga atgcgggtaa
     1081 ccggcaggcg gatgttgcgc nnnnnnnnnn nnnnnnnnat atctccgata cagtgaaaat
     1141 tgaaccgtgg gataactata cccttctcgc ggtgcaaggg ccagatgctc caaaactttt
     1201 agaggcaaaa tttccggaga ttaaaaatct ttactattac gaagttgcgt tccgtggtga
     1261 agacaccttc atcgcccgca tgggctacac cggcgaagac ggttttgaaa tcgcgtgccc
     1321 gcaggcagag gggattgcgc tttggaaaga gcttattgca gcaggggcaa aaccatgcgg
     1381 tttggccgca cgcgatgtgc tgcgcctgga agcgctcatg ccgctttatg ggcacgagct
     1441 ttcatcaacg ttgcgtccgc aagaatcggg actgggattt ctctatagcg accacacatc
     1501 gattgcgccg gcggcattgc gtaaccaagc tacggcgcaa aatagttttg gattccaaat
     1561 gcaaggtgag gggattcccc gccaaggcta taaagttgtc cgtgaaagtg gagaagtcgt
     1621 gggcgaagtc acgagtggta cgttttcatt cacccaaaat tgcggcatgg gttttatgcg
     1681 catcgacatc gcacaaaaag acaaggcatt atttgtggaa atccgtggtg aaaagaaacc
     1741 gattgccgtg ttagaaaaaa ctccgataaa gggcagtgtc cgtcgacgac ctaaataaaa
     1801 acatcgaagc acgtacagcc acgccccaaa aacgcgcgca agagctcatc cgcgagttcg
     1861 tcgagttttc ggaaaagtat agcgcgcaaa tcccgctggc aatacccaag gggactctca
     1921 agacaatcga tcactaccgc gacacgtatt ttaagtatct taaagactcg gagttcaaat
     1981 cgaacatctg ctatatgttg cagctcattg agtatcaact ttggcaatat aaacttttta
     2041 aaccgcaata ctccctcgag aatgcgcttt tttttcagtt actcatcacc caaggcattg
     2101 tgattgaggc ggtggtgttt gctctcatcg ccgacccttt ggtgacgagc gataaactcg
     2161 accgctcaaa aggctcgaca aacgacgagt attcgcttct tttgaatctc atccgccgcc
     2221 gcacgttctc ctcgcatgtg caacaactgg cggcgatgga aattttgagc gaacccttgc
     2281 gcgaaaagct cgacacgttc cgcagcgaaa tccgcaattt ggtacatctc caaaattggg
     2341 atggccggct ctatcgacaa attacgctgg tcgaatacac aaagcaccta cacgaattcg
     2401 atgtactcct caaagaatta aacgcgcaag tgacggcggt acacacgctc gacgtgctgt
     2461 tgcagtatta tcaactcgat ggacgtagca tcaaaggtat tgttaaattt tataaccgca
     2521 acagccgacg cggacagatc gacgccaaaa gtgtccatga ttatattccg tttttttatg
     2581 aagacagttt tggcgattgg ctcccacaga agaacgacgt tgtttttttt acactcgctt
     2641 taaacgaaca tggcgttcgt gcaatccaag ttaaaccgaa ccgttagttt agtcggtaaa
     2701 cgatgcgcca atactctggc tgagtttctc aacaatacgt acaagctgca ctgaaagtac
     2761 tccttgggtg ctgttgagta tctctcctaa atctttataa ggcacacgga tggataaaag
     2821 gctgcgcttt tgttgagtaa ggagactttc ttggcgtttt ttgcgtttca ttaaggattg
     2881 aatcaagtcg ataatttgtg acggcatcgt ttggggaaaa aaattttcat gccacatttc
     2941 gcgctttctt tcaatttcag gattctctcg aaaaaaagcg ttgatcttcg attcgatgag
     3001 tgaaatacct tgaaaattgg atgtcagctt gtcttcgtaa tcgcgcacct cgtttgatcg
     3061 ttcgagcgca atccctttta atttctgtac tcgctttaga atttcaacct cggaggtctt
     3121 ttttaggtct ttgaggtact caatttcatc gacggtgagc tcaaagtacc acgcgtacgc
     3181 taatttgaag agtactcgtt ggttgatggg ctgcgcggcg aggctttcgc taaatttatg
     3241 gaacagactc ccctcggcta aaccctcttc acgggtgccg acatcggcgt cgaccgcatc
     3301 gagtaactta cctgtgccgt cgaacttgcg gttatgcaca aaattttttt tgtctttttg
     3361 ggtacgcaaa aaatcgattg ttaaattacg caacaccgcg tagaaccagg ttgtgaactt
     3421 ggctttccct tgaaagctgg ttatttttcg tccatcgcgt aaatgctcga acgcatagag
     3481 ataaaactcg ctcgcctcgt cttcggtgaa gcggaagagg cgtattggaa agttgtagat
     3541 atcgtcggag tatatttcaa aaaatgcggc caacgcatct gagtcacctt tctttatgcc
     3601 ctggatgagc tcaagaacaa tctggtcgcg ctccctgtcg gctgtgctct gttttgccat
     3661 ctttcttttt ttacagagcc ttaatcgaga ttgttttttc tataggctgt ttaaaaagat
     3721 aggctttaat gttattcgac ggtgactgct ttcgccaagt tacgagggcg atccacatcg
     3781 cagccgcgct cgatggcggt gtagtacgcc aacaactgca aaggaattgc caccaaaagg
     3841 ggcgagatac tttcgtcgca cgcggggatc tcaaaataaa aatcggattc ctttttaatt
     3901 tcgtcgtcac cttctgttat tatcgaaata atcttgcctt ttcttgattg tgcttcttta
     3961 agatttgaga tcatttttgt acggatttcc ccttggggcg caatgataac aactggtacg
     4021 ttgtcgctga taagcgcgag aggcccgtgc ttaaactcac cgccggcata ccctgatgcg
     4081 tggatgtatg aaatttcttt aagtttcaac gcaccttcga gagcagaggg gtagttatac
     4141 gtgcgcccta agaaaatagt gtcgttcttg tcttttaaga actttgccat ttctttaatg
     4201 gagtcgtggt ttttgagcat tttgcgcatc atgtcgggta tgctgcgtag ttcgctcaat
     4261 aacttcgcgc gctcctcgga agggatgagc catttaatcg agccaacata aagagagaaa
     4321 agaaaaagat tcaagacctg cgcggtaaac gctttagtcg aagcgacacc gatttcaggg
     4381 cctgccataa ggttgacgag tgcatctgac tcgcgcgcca ttgtcgagtt cacgttgttt
     4441 aagaaagaaa gcacgcgcat aaattttgcc ttcgcttcgc gcagtcctgc gagagtgtcg
     4501 gcggtttcgc ccgattggct aatgcccatg acgagagaat cgcccgaggt gagagggttg
     4561 cggtaacgcc attcggagga atagtcggtt tcggtgctaa tccgcgcaaa ttgctcgatg
     4621 tacatacggc cgatcatgcc tgcattgagc gaggttccac acgcttggat gagaatgcgc
     4681 cccacttggg cgaggtattc tttcccgaat ttcagttcgg gaaagtgaat aaacgtgtcg
     4741 tctaaacgtt ctttgaaaat acgctcgaaa acatcgggct gttcgtgaat ttctttgagc
     4801 atcaagtagt caaactcgcc tttatcgaca tcctcgacgc tcaccgagac ggtttcccat
     4861 tttggggtga cagggtcgcc cgcagcgttg aagatagtta tcccttctgg ggtgatatac
     4921 ccccattcgc cgttttccaa ataaatcgct tcgtcgcaaa tcggtaccac cgccggtata
     4981 tccgaggcga ggatgtactc gttattcccg cgcgcgatga tgagcggcga gcctttgcgc
     5041 gcaaaataaa ttttgcggtg tgctttttca gaaatcaccg ccaaagcgta ctggccttct
     5101 aaacgacgca gtgtgcgcgc gaacgcttca tcgggagaaa agccttcttc taaatattta
     5161 tccatcaaat gcggcgcgac ttcagtgtct gtttcgctca caaaaacata accatcgtcg
     5221 gcgagttctg cgcgcagctt ggcgtagttt tcaaaaatgc cgttgtgcac caccgcgatg
     5281 gtgctgtctt tgttgatgtg cgggtgcgcg ttgactgtgg aaggttcgcc gtgcgttgcc
     5341 cagcgcgaat ggccgatgcc gacgctgcca aacatcggct gacttttcag ctttttctgg
     5401 agttcggcga ttttacccac cgctttacga gttgtgattt cgtcgccgtc caaaaccgcc
     5461 atacccgccg agtcgtaccc acggtactcc agcttttgca aacccactaa aagtaccgat
     5521 tctacggatt tagggccaat atagccgaca attccacaca tactttggca ttcttcttta
     5581 cgtagagtcc gcgcaaagta caaacggtca ttatggcaca agtaacattc catgaaaaac
     5641 ctgtccaaac ggcgggtaat ttaccagcgg tgggctctaa aatccctgat tttcgtctca
     5701 ccaaacaaga tctttccgac ttaaaatctg ctgatttaat aggcaagaaa cttgtattta
     5761 atattttccc cagtatcgac actcccacct gcgccaccag cgtgcgtaag ttcaacgcgc
     5821 aagcggcatc gttgcccaac acggtcattg tgtgcgtatc gtcggattta ccctttgcac
     5881 aaaagcgttt ttgtggtgca gaagggttga ccaacgtggt gaccgcttcc gatttcaaag
     5941 acaaaaaatt tggtcgcgat tggggtgtct atttcaccga cggtccactc caagggctca
     6001 tgtcacgggc ggtggtcgtt gccgacgcaa cgggcaaagt ggtttatacc gagcaaggcg
     6061 cagaggtggt caacgaaccc aactacgaca aagcactcgc tgcggttaaa agcgcataaa
     6121 atttaatcga taaacacgcc gagcaagagt tcggcgtgct tataatcttt ccgtattatc
     6181 tcctatagtc tttcaaaaat ataccgcgat gaagaaacga ccatcgattt gctccacttc
     6241 gaggccgata aattgaaatt gctcaaagca tggaacttac gaccttcttg cttaaatcat
     6301 acctcggcaa aatccaaaat ataagatttc ttccataagc catgtaatac tcaagccgcg
     6361 ttcggttaat tttttcatgc gttgcagcgt tttgagcgtc aataccgccg catacgcttc
     6421 gcgctgttcg acctgtttga gcgactgcgc cttacgatgt ccatgcgctg ttgtgtagta
     6481 cgttggagta acgctgaaat cacggtaacg ctcgtgctgt aacgtgtcgt aaaatcgttc
     6541 cgccataagc tcaagctgct gcggcgatgc aatagccgcc agagcccgcc gcgccggtgc
     6601 tgaaagctgg aatatcattt gtaatgttgt tgtcatatat agattaccct ctatatatta
     6661 aataccaaaa aacgctcaaa ttttctcact ttttgaaaaa aaagtgcaag ttttgtagaa
     6721 aaatactgat ttttttaaaa aatttgtctg ttaggatagt tatccctcaa tgcgacataa
     6781 taccggatag ttatccctca atgcgacata ataccggata gttatcccta atgcgacata
     6841 atacaataac cgtaatcgtt caccgccatg aatataaaaa acgtaaaatt tgctcgccta
     6901 tgagcctgcg tgagttggcg cacaggctcc tatacgtgat cactgttgtc cattccatgt
     6961 tgtgttaatt tttagtcata aatttccgat aatgaaaaaa gacataccat gccgctattg
     7021 agaaaatatg tactttttgc atgctgttat ctcttcattg gcgtagcggt tctctacaca
     7081 cactatttac aaacctctcc gtatcaaaaa gatgctctcg atgaaaaatt gactctcgtt
     7141 gccgcatcgc atttttcgct gccgcaactt gtcgataatt tgccaaccgt gtttcgtgaa
     7201 aattcccacg cggcggcgct caaactcaca aatttcaaag gcgagttttt gggggcgatg
     7261 tacgatgcac ggcggatgcg gagcgatgag tatagacaat ttttggatac aaaatcgttc
     7321 actgtacaaa aatctccttt gataggatat gaaattttaa tttgggagag taaggggcga
     7381 aagttgcgca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 ggggcgatga gcaatacata cgcgctgcac tatcttatac cgatttatgt tttattcggt
     7501 ggcttgcttc ttttcatgta ccattggttt tggggaagcg aaagtcattc tcggcattta
     7561 ttttcagaaa agaaaaagcg tcttcctttt tttgcacccg caaaaactac agtggattcg
     7621 gcagcggcgc tttctcccac agaacccaag caccgttggc agcttaagtc gggggtcatc
     7681 gccgaagggg gcatccacgc aacgctagct cagttacgct cgctcgccgg cgccgaatcg
     7741 gtgcggctgt ttgcaaaact aaacaaccag tggttgcctg ttgccgaggt gcgcggccag
     7801 ttgacagtgc gcggcgaagc aatggaatta ccttctctac tcacacagcc gtttgcatct
     7861 accgaggaat ttctttttga cacagtgtat ggcgaatgtc ttgcctttaa caaggcgcca
     7921 caaaatgcag aactctgttt tgttttgaaa ctcaattcca ctaaagaaat cgcagagagt
     7981 gaaatgcgag aacgcattgg tacgtttgtc aagacgcata gccgttcgtt gctcgttgaa
     8041 cattattatg aaagttcaat tctcgatgca gaaagcggcc tttacagttt tccgtatgca
     8101 atgtttactt tgaaagagaa aatattaggc gggctcactt tcagtgccgc tgttttccag
     8161 tttccagaaa gtttttttac gtcgagccat ctttcaaaaa ttgcacgcac tgcgattcga
     8221 gcgatgagag agtatttcgc ggcagacgag gctccggtga ttgcacgcgg cagcggcaat
     8281 cgcctcttta tcatttttaa cgcacgccgc gaaggcgcta aagattatgc tgaagaggca
     8341 acgctgaaat tatttacctc gtatgaaaat attttcaaaa cggaatttca actccaggca
     8401 ggctttgtcg ccgacgccgc agggtttggt tcaagcacgc gcgctataaa aattttagag
     8461 cgccttttgg atgaatcc
//
LOCUS       DAOMPD010000046         8235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3741_length_8235_cov_2.827506, whole genome shotgun sequence.
ACCESSION   DAOMPD010000046 DAOMPD010000000
VERSION     DAOMPD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_8235_cov_2.827506"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLY93_02660"
     CDS             complement(<1..245)
                     /locus_tag="PLY93_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68412.1"
                     /translation="MKKIFYIYFALTLASASTLFAAKNTTVKEMPPVTAAVPAAAPVE
                     SQRTPLPDATAWSFHTGSYAGNGILGGSLQFGLASWFR"
     gene            301..1494
                     /locus_tag="PLY93_02665"
     CDS             301..1494
                     /locus_tag="PLY93_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68413.1"
                     /translation="MKQPWIYNVWIDSLFILGIPFFITALILLLPASFRAPDSMTVVS
                     WALLVVAVDVSHVYSTLYRTYLDKETRNQHSVFLYIMPFFVFVGCFILYSLGAMVFWR
                     VVAYLAVYHFTRQQYGFLRIYSRSENASAWARRIDALTIYSAALYPILDWHLSGQKSF
                     NWFMQGDFYYFASSTAKAWALGAYLSVLGIYVVKEILQLWKNRTIAMEKQSYPLLFNI
                     PRNTIILGTIISWYIGIVYLNSDLAFTAINVVSHGIPYMALVWIWGRKKYENIVNNEM
                     GKEKTPESTTTKTAYHAQSAWAFIFRPRYVLLFIAFLFLLGYIEEGFWNIWVWHEKEH
                     VAFFSFFHKLSGNTIYGNDWLKFAVPLLSVPQITHYIIDGFIWKIRKDKYAWQVTLSS
                     TKEVK"
     gene            1719..2336
                     /locus_tag="PLY93_02670"
                     /pseudo
     CDS             1719..2336
                     /locus_tag="PLY93_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
     gene            2360..3259
                     /locus_tag="PLY93_02675"
     CDS             2360..3259
                     /locus_tag="PLY93_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRP68414.1"
                     /translation="MRTTLKILTLTALTLLYSVSAAPAQKSGSTIKTLNGDDTDGMEI
                     RSIQVEGFESEPWTATVSPGDPEAIPAVVDQQGVPQQGEKWNSPEVGLKGTTQQKCRP
                     QNLAYDETNNKCLGVRFKFIYPGNNVVTLTPPASRKIYRVLDELDHNNKPKVQEIHGL
                     KLPGKVKAISVWVLGRGNDYTLEAWVQDWKGSTHVLKMGSVNFVGWRPLTVRVPANVP
                     QDIDTYPQTKNLILTKLVLRSKPTANTEKVVIFLDAVKVLTDVYDVYFDGADVDFDEQ
                     DKKEKAEAKEYAKQLKKYSEGIK"
     gene            complement(3378..4873)
                     /gene="carB"
                     /locus_tag="PLY93_02680"
                     /pseudo
     CDS             complement(3378..4873)
                     /gene="carB"
                     /locus_tag="PLY93_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728238.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     assembly_gap    4874..4892
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4893..6764)
                     /gene="carB"
                     /locus_tag="PLY93_02685"
                     /pseudo
     CDS             complement(4893..6764)
                     /gene="carB"
                     /locus_tag="PLY93_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137995.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            6952..>8235
                     /locus_tag="PLY93_02690"
     CDS             6952..>8235
                     /locus_tag="PLY93_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68415.1"
                     /translation="MAYTFHVHQNMPEDAFLFMEFQASNQMRWAADYLKAKAEGRYSG
                     VCVTLGGDASGESLNIQCGAIGINRVGADKPDFVQDVFINHPILPFNWNAVGINVTSY
                     THFINLKKKNANGNDIVINNYNIFDGYAYNTTYGFDEFGSMDSLIAVGLNASDFSIDP
                     LGCTDSACSEYLGLSFALKENPTVDYMQNNSTTPLGNPSGSKRLGNDPLSNYNCLSDT
                     AVIGPCNDEGVKVGGIYQIPNVRRITDAIEIAESFFVNNQDWITWEPSSNAATFYFNE
                     AWLEGLQSRNHSLQTAKAVGRYYSVSGDQVLWYTPSHHYLGDLVQMTHVWISTGYNHT
                     DIEGWADDQYGKRQVGGSNPNQNYEDYVNTQAYANSLQNRYNLPIGEVYKILFEQAFH
                     TYHIRYRAGYDKMTTSNKTVWKNFVTWAVQNVHASM"
BASE COUNT         2170 a         1922 c         1939 g         2185 t
ORIGIN      
        1 cgaaaccaac ttgcaagacc aaattgtagc gaccccccta aaatcccatt gccggcatac
       61 gaaccggtgt ggaaactcca tgcggtggcg tcgggtagtg gagtgcgttg gctttcgacg
      121 ggtgcagcgg cgggcactgc tgctgtgaca ggaggcattt ccttcacggt agtattttta
      181 gctgcaaaga gagtcgatgc gcttgcgagt gtaagcgcaa agtagatata aaatattttt
      241 ttcattgtgt acgagagttc attttttagc tttactgtaa atagaattta gtaaatgaca
      301 atgaagcaac cgtggattta caacgtttgg atcgatagcc tttttattct cggcatccct
      361 ttttttatca cagcgttaat cctactttta cccgcgtctt tccgcgcgcc cgattcgatg
      421 actgtagtga gttgggcgct tctcgtcgtc gcggtcgatg tcagccacgt atatagcacc
      481 ctttaccgca catacctcga caaagaaacg cgcaaccagc acagcgtttt cctttatatt
      541 atgccttttt ttgtttttgt gggttgtttt attttataca gcttgggtgc gatggtgttt
      601 tggcgcgtgg tggcgtatct cgcggtgtat cattttacgc ggcagcagta cggttttttg
      661 cggatttatt cacgctccga aaatgcgtcg gcatgggcgc ggcggatcga cgcactcaca
      721 atttatagcg cggcgcttta ccccatcctc gattggcatt tgagcgggca aaaaagtttt
      781 aactggttca tgcagggaga tttctactat ttcgcgtcga gcacagccaa agcgtgggcg
      841 ttgggcgcgt atttatccgt tttaggaatt tatgtggtta aagaaatact ccagctctgg
      901 aaaaacagaa ctatagctat ggaaaaacag agctatccgc ttttgttcaa catcccccgc
      961 aatacaatca ttctcggcac catcatttca tggtatatcg gcattgtgta cctcaattcc
     1021 gacctcgcct tcaccgccat caacgtcgtg tcgcatggaa tcccctatat ggcgctcgtg
     1081 tggatttggg gacggaagaa atacgaaaac atagttaata acgaaatggg taaagaaaaa
     1141 acaccggaga gcacaacgac aaaaaccgcg taccatgcgc aatccgcctg ggcgtttatt
     1201 tttcgtccac gttatgtact cttgtttata gcgttcctct ttttattggg ctatatcgaa
     1261 gaaggctttt ggaacatttg ggtgtggcat gaaaaagaac atgttgcatt tttttctttt
     1321 tttcataaac tatctggcaa cacgatttac ggcaacgatt ggctcaaatt tgcagtgcct
     1381 cttctttcgg tgccacaaat cacgcattat atcattgacg gctttatatg gaaaatccgt
     1441 aaagacaaat acgcatggca agtgacgctt tcttctacga aagaagttaa gtaatgttga
     1501 atgttatccg atagtagtaa cagtataaaa ggcttttcaa gacgtataaa gcaaagtctt
     1561 taggtaaata catgggggaa tttatggatc gactcggttc agcgcgagta gttacaagcg
     1621 gtaaaaaatt acttttaatc acggtattgg cggcggcgtt tgtttcagta acaatcgttg
     1681 cgcaagatgc atctaacaca aaatcaaaga aagcagacgt gcaagacgtt ctcttgaacg
     1741 actttgaatc ttcagaagat tggcgcgcat tctcaacgac accgttgggg gttacgaaag
     1801 tacgtaaaac cgtccaactt gggccgattg aagacgtgtt taaccccaag gaattatccg
     1861 aagaagaaaa agcgcggttt aaagacggat taaacgaccc taaatcagcc aacaacgtat
     1921 tgggtgttaa aactttcttc aaagaaaaag gctttgaccg tgttgaaatc aaacccccgc
     1981 acgaatacct catcaaaggt ttggggcgtc agctttcggt gtgggtgtta ggacgcaatt
     2041 tccgccacac tctttttgta aaattacgcg actacaaagg caatctgcat aaacttaaat
     2101 taggcaaact cgactttttt ggatggcgca aactgacggt caccgttccc ggttggttac
     2161 cccaaagcac ccgccacgcg ctcttagaca aaaacttaca ctttgtttct ctttatgtag
     2221 agtgcgatcc acacgaaact tggggtgatt tctatttcta cgtcgacgat ctgcgtatta
     2281 aaacagattt gtctgacagc gaataccccg gcagcaaaat caaggataac tggtaagtca
     2341 tagatcacgg aggatcacga tgcgtactac attaaaaatt ttaacactca cagcgttgac
     2401 gctgctctat tcagtgtcgg ctgcaccggc acaaaaaagt ggcagcacca taaagacctt
     2461 aaacggcgac gacaccgacg gtatggaaat tcgttcgatt caagtcgaag gttttgaatc
     2521 cgaaccatgg actgccaccg tttcaccggg cgaccccgag gcaatacccg ccgtggtcga
     2581 tcaacaaggc gtgccacaac aaggtgaaaa atggaactca cccgaggtgg gtttgaaagg
     2641 caccacacaa caaaaatgcc gtccacaaaa cttagcgtac gacgaaacca acaacaaatg
     2701 cttgggcgtc cgctttaaat tcatctaccc cggcaacaat gtcgtgactc taaccccacc
     2761 cgcttcacga aaaatatatc gtgtgctcga cgaactcgac cacaacaaca aacccaaagt
     2821 acaagaaata cacggcttaa aattgccggg taaagtgaaa gcgatttcgg tgtgggtgtt
     2881 gggtcgtggt aacgactaca ctttagaggc gtgggtacaa gattggaaag gctccacaca
     2941 cgttttaaaa atgggcagcg tcaactttgt gggatggcgc ccactaacag tgcgggtgcc
     3001 tgcgaacgtg ccacaagaca tcgatacata cccacaaaca aaaaatttaa ttttaactaa
     3061 gttagtgttg cgctcaaagc ccacagcaaa cactgagaag gtagtaattt tcttggatgc
     3121 agtcaaggtg ctgaccgacg tttacgacgt atacttcgac ggtgccgatg tagactttga
     3181 cgaacaagac aagaaagaaa aagccgaagc aaaagaatac gcgaaacaac taaagaagta
     3241 cagcgaaggt ataaaatagt taaaatcaaa ttacgcctaa ttgccgcttg ctcggcgatt
     3301 aggcgctttt agtcgcgctg ggcaatgaaa tgggcgcgaa tttgtcgtgt ttaaaaaatc
     3361 gttctataac tagtattcta taaagcgtaa atttcttctt tcttgccgtt catctcgaca
     3421 atgcctacga gcgcttcgcg cgccgcctcg acggtggtaa aatacggtat cgaatactgc
     3481 agtgcggcaa gacggatttc caacgcatcg ttacgcgttt ttacgtctgt gggtatgttg
     3541 ataataattt ggatgtctcc cgacttgacg ctgtcgacaa tgtgcggtcg tccttcgtgg
     3601 actttattca cgcgctcggg ctttacgcct gcttgtgcaa gcacagttgc cgtgccatcg
     3661 gtggcgacga ttttatagcc cgctttttcg gcggctttga gcgcgggaat gagcggcgct
     3721 tttgcgatgt cgtttaccga aacaaagatt gcgcctttgt cggggagctt ttgccccgct
     3781 gcaagctgcg ctttgtagaa cgcctctgaa acggttttgc cgatacccat cacttcgccg
     3841 gtggatttca tctcgggacc taagatggtg tcgacccccg caaatttttt gaacggcaac
     3901 accacttctt tgacatgata aagcggtgga ataacttcgt gggttaaatt gagcgatgcg
     3961 agggtgtatc ccaacatgat cttggtggca atttttgcca aaggcaaccg cgtcgctttc
     4021 gagatgaaag gcacggtgcg cgatgcacgc gggttgactt cgagaatgaa acatccgacc
     4081 ctttcgcagt tgtaacgatt gcaaactgga tgttgataca acccaccact ttgagttcga
     4141 gcgcaatcgc acgcgtcacg cggcgcagct cgctaaggag ttcattggat atatttttgg
     4201 tcggcaggat gcacgcgctg tcccctgaat ggacacccgc ctcttcgacg tgctccaaaa
     4261 tgcccgcaat aaaaacatcg ttaccgtcgc agagtgcatc gacatcgact tcgagtgcac
     4321 ccgacaaaaa ttcatcgatg agcaccggac gttcgcggct cacctcgacg cttgtggcca
     4381 tgtacgtgcg caattgcgcc tcggtgtgta cgatcatcat cgcacgccct cccaacacaa
     4441 acgacggacg ggcgagtaca ggataaccaa tttcatcggc ggctttgagc gcctcttcga
     4501 gcgaagcggc catgcggcct ttgggttggt tgagttttaa tttttcaatg agttggttaa
     4561 acagttttct atcttcggcg cgttcgatac tttcaacgct ggtgcctaaa attgacacac
     4621 cttcttggct cagcgccttc gcgagcttca acggcgtttg tcctccgaac tgtaaaatca
     4681 cgcctttgac attacccgca gcgcactcac cgcgataaac atgcatcacg tcctcggcgg
     4741 tcaggggttc aaaatataag cggtcggaag tatcgtagtc ggttgaaact gtctcggggt
     4801 tggagttcac catgatactc tcaaacccaa tttcttggag cgcgaaactt gcgtggcaac
     4861 aacaataatc aaannnnnnn nnnnnnnnnn nngcagtagt cgaactcgat gccctggccg
     4921 atacggttgg ggccgccgcc caaaatcaac actttatcgc gagcagatat atccgcttcg
     4981 ttttcggttt cgtacgtcga gtacatgtac ggcgtaaacg attcaaactc ggcggcgcaa
     5041 gtatcgacac gtttatacac ggggatttct ttttttgcca agcggtcttt tgtaatggcc
     5101 gcctgtgttt ctaagagcaa tcggttgagc tcgtacattt tatccatgaa cgccgacgag
     5161 cctttgtcta accgcacaag ctcttcggca aagcgagcaa ccgtatcgaa gagtttgcag
     5221 tacgcgagtt ggcggtcgtg gaagcctgct tgctttgctt cacgtaaaag ttcctgtgga
     5281 agtgtaccat cttttttata agcgtcaatc acgcgcgatt caatcgttag aatacgctta
     5341 agctgtgtta aaaaccaagg gtcaatcgag ttggcacgat aaatttcttc gatgctaaaa
     5401 ccgcgcacca aggcttcgcg caagtagaaa atccgttgat cggtgggggt actaattttt
     5461 tataaaatat gtttgcgttg tgcggcttca tccccatgcc gcagaatttc aatcgcgata
     5521 ttgaggtcgc catccccccc ccaaccgaaa cggccaattt ccagcgaacg catcgctttt
     5581 tggaacgact ccataaacgt gcggccaatc gccatcgctt cacccaccga acgcatttgg
     5641 gtgtccaacg tgcgcggtga ttcgggaaat ttttcaaaag taaagcgggg tatcttggta
     5701 acgatgtagt cgatagtggg ctcaaacgac gcgggtgttt ttttagtaat atcgttggga
     5761 atctcatcga gagtataacc caccgacaag agcgcggcga ttttagcaat cggaaagccg
     5821 gtcgctttcg aggctaacgc cgatgagcgc gaaacgcgtg gattcctttc gacgaccatc
     5881 acttggccgt ctttggggtt caccgcaaac tggatattcg atccaccggt ttcgacacca
     5941 atttcacgga taatttttat cgagaggtcg cgtagatttt ggtattcggc gtcggtgagt
     6001 gtttgctgcg gcgcgacggt aatagaatcg ccggtgtgca cacccatcgg gtctaaattt
     6061 tcgatactgc acacaatcac cacgttgtcg gccttgtcgc gcatcacttc aagttcgtat
     6121 tctttccagc ctaaaatgct ttggtcgatt aaaacttggc tcgtgggact ttccgacagc
     6181 gcctttgaca ccattgaaat aaactcttct ttattgtagc aaacgccgcc gccggaacca
     6241 cccagcgtga acgaagggcg tagaatggca ggaagaccaa tggtgtcgag cagaacaagc
     6301 gcttcttcga gcgtgttggc aatccccgag cggggcatcg ctacgtctat cttttccatc
     6361 gcttctttga agagctttct gtcttcggct ttgttaatcg ctttttcgtt ggcgccgatg
     6421 agctcaacac cgtatttttc gagcacacct gctttagcca aagacagtgc agcgttgagg
     6481 gcagtttggc caccaaccgt gggtaaaaga gcatcggggc gttcttgttc aataatcttg
     6541 gttaaatagg gtagagttat gggttcaata taaacgcggt cggcgagttc agggtcagtc
     6601 ataatcgtcg ccgggtttga attgatgaga acgacttgaa atccttcttg tttgagggct
     6661 tttaccgctt gtgtgcccga ataatcaaac tcacaagcct gaccgataac tatcggcccc
     6721 gacccgataa tcatcacttt tttgatgtcg gtacggcgtg gcacacgtca taccatgttt
     6781 ttgagacaga ctgtcagctc ttagataaga agtttgtcag aatttatata aaacccacaa
     6841 aaatggccta ctttactctc cccgtgcaat atatagatga ggtaaataga cgtgtatact
     6901 cacttaagat tttttctact ttctttttca ctggctgcat acgctccact catggcgtat
     6961 acgttccatg tgcaccagaa tatgcctgaa gacgcatttc tctttatgga gtttcaagct
     7021 tcaaatcaaa tgcgttgggc ggctgattat cttaaagcta aggcagaagg ccgttactcg
     7081 ggggtttgtg taaccttagg aggcgatgcg tctggtgagt ccctcaatat acaatgcgga
     7141 gctattggga tcaaccgtgt cggcgccgat aaacctgact ttgtgcaaga tgtattcatt
     7201 aatcatccta ttcttccttt taactggaat gcggtcggta tcaacgtcac cagctacacg
     7261 cactttatca accttaaaaa gaaaaacgca aacggcaacg atatagtcat caataattac
     7321 aatatttttg acgggtatgc gtacaatacc acgtatggct ttgacgagtt tggtagcatg
     7381 gattctctca ttgcggtcgg tttaaatgct tcagattttt cgatcgaccc attgggatgt
     7441 accgactcgg catgttctga atatttgggg ctaagttttg cgctcaaaga gaacccaacg
     7501 gtagattaca tgcaaaataa ctcaacgaca cctttgggta atcccagtgg ctcaaagcgt
     7561 ttagggaacg atccactttc taattataat tgtctcagtg atactgcggt tattggccct
     7621 tgtaacgatg agggggtaaa agtcgggggc atttatcaaa taccgaatgt gcgccgcatc
     7681 actgacgcta tcgaaatcgc cgaaagtttt tttgtaaaca accaagactg gattacttgg
     7741 gaaccttctt ccaatgcggc aactttttat tttaatgaag cgtggcttga aggcttacaa
     7801 agccgcaacc atagcctaca aacagcaaaa gcagtggggc gttactatag cgtatcgggc
     7861 gatcaagtgc tttggtacac ccccagccac cattatttag gcgatttagt tcaaatgacc
     7921 cacgtatgga tttcaaccgg ttacaaccat acggacattg aaggatgggc tgatgaccaa
     7981 tatggtaaaa gacaagtggg tggttctaac ccaaatcaaa actacgaaga ttatgtgaat
     8041 acacaagctt acgctaactc gttacaaaat cgctataatt tacctattgg cgaagtctat
     8101 aaaattctct tcgaacaagc gtttcataca taccacatac gttaccgtgc cggatacgat
     8161 aaaatgacga catccaataa aacagtttgg aagaattttg taacatgggc ggtacaaaat
     8221 gtacacgcgt cgatg
//
LOCUS       DAOMPD010000047         8197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3772_length_8197_cov_2.463277, whole genome shotgun sequence.
ACCESSION   DAOMPD010000047 DAOMPD010000000
VERSION     DAOMPD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3772_length_8197_cov_2.463277"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>414
                     /locus_tag="PLY93_02695"
     CDS             21..>414
                     /locus_tag="PLY93_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68416.1"
                     /translation="MNLFKKIFNSSRELFVASDPMLADLGHEGEFIMARIRFILILLV
                     AVIPLKTILFEESSFENWVGVIVALIALLFATLILIGVRKKVPSRSISWISTQTDVLL
                     VTSAEVGFLLSPTPIIASNNMVAFGLYLI"
     assembly_gap    415..452
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            661..1112
                     /locus_tag="PLY93_02700"
                     /pseudo
     CDS             661..1112
                     /locus_tag="PLY93_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1158..2186
                     /locus_tag="PLY93_02705"
     CDS             1158..2186
                     /locus_tag="PLY93_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68417.1"
                     /translation="MEKNERILSLEDDVEYARVLSQALRIFGEVQFTAYPDRFLSLIE
                     TFKPTLLISDYNLNHPTLNGIKVTRMVRRDANSNFLPILLLSGEEDMEVIEEAYKSGI
                     DDYVLKPVIPRFLIAKIENLLFQTRKKLNAQALSGLPGNSAIETEFYLRWQSKKAFTA
                     AYTDLDNFKPFNDEKGVKKGDEAIQIVSEILYQLRAETERHRLFVGHIGGDDFFLMGG
                     LTSITSAVKKLHKRFALESRRFFSAPELKSGFYRGTDRQGNFCSFPLLTISTALIKNI
                     SYTTLGNFTQLTELAARAKKAAKKSTNKFVIYDVAVLSVPQKVQNLFELQQNKGQKKQ
                     QFRRQKLK"
     gene            2243..2542
                     /locus_tag="PLY93_02710"
     CDS             2243..2542
                     /locus_tag="PLY93_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68418.1"
                     /translation="MQYVLDLLVYVVLGAVLSWFFYYLKRLDIFGGFLGGALVAVIGA
                     ILGAFLLQVPLKFLIDALQNGFYISNVNVIAALIGGVASLWLLSKINNGRKRKDY"
     gene            2539..3018
                     /locus_tag="PLY93_02715"
     CDS             2539..3018
                     /locus_tag="PLY93_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68419.1"
                     /translation="MKRIGVFIACGILGCVPSKLTHPDLTRYFAKAEYIPSKGIGADG
                     WAYARRRRLLKRYLQALHIDFIEKDRVAPTPQNKYRDIVLAVSGKGDKKIMVTLALQA
                     HTSGNPTDEFIKAQHECDTYLFTFLSTLEQRLLRWESIEFTQTETCNRLTAHDGHEK"
     gene            3022..3957
                     /gene="ispH"
                     /locus_tag="PLY93_02720"
                     /gene_synonym="lytB"
     CDS             3022..3957
                     /gene="ispH"
                     /locus_tag="PLY93_02720"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRP68420.1"
                     /translation="MKEIVIAAPRGFCAGVDRAISIVERALEVFGTPLYVHHEIVHNR
                     YVVDSLRAKGVVFVDAIDEIPPNQNVIFSAHGVSPAVTAAAKEKNLKILDATCPLVTK
                     VHIEARRYARDGYQIVLIGHKHHIEARGTYGEAPEQITIVETLADIDALNFSPDAKLA
                     YITQTTLSVRDTKGLIERLKERFPQIEGPSSSDICYATTNRQEAVSVIAPEADIMFVI
                     GSKNSSNSNRLKEIAEAQGTKSYLIDNADDLKPEYFTDAVQKIGITAGASAPEILVKQ
                     VTDRLRNEFGFTGIKEVRLKNEDVTFKLPVELRAT"
     gene            3954..>4634
                     /locus_tag="PLY93_02725"
     CDS             3954..>4634
                     /locus_tag="PLY93_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68421.1"
                     /translation="MKRYFLFYIFFFTPLVAEVFDEVKSNTKAGIAAYQTRRLAEARQ
                     FLSTALNIDSTWMPAVLYHGEAFADYLTAELYIHNTQKYIHFENFPNAESALDEAEGY
                     YPAHPQIPILREKIRKAREANTKIILAKLPEAKRKKYEASMEAAKLAMNQSKYAEAMH
                     HYSLALKIAPQSLDAQMGYAEAERFFKQDGKEEKISTLFQQAEKHKKERRFAQAVSNY
                     DRILRLESN"
     assembly_gap    4635..4644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4645..5819
                     /locus_tag="PLY93_02730"
     CDS             <4645..5819
                     /locus_tag="PLY93_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68422.1"
                     /translation="NKEALTNKAALVDLMQQQLNAEERKNLAKEYLVSGNDAFKRHDF
                     AFAIEQYRLGKAMDPRIADWDALLKKAIAAKKKQDENLFVDKVKELEQRYQAATLQML
                     LENFALAVENLEAVIEIAKELKQNETQKQAEELLVIAKQAMLRQDQEYITRDSPYYSF
                     VQSLTALGLNSYTNKDCSATLKHFGAIVQIFPKHRVSNQHIMSCTIILHPERKESIIQ
                     ELIESIYRMKDANPFEARRYFDILKFIDPKNPKIPELERELAEKTQVLKKSVKSAEYI
                     DALYRRALVLSQSDPRAALDILRQLLAEDPANGKARALFARIEARIKRQEWADSDRPV
                     APEALKAYADGIVYYNTGQILQAKAAFSQALNLAPNFERAQVALKKCESYSKGARF"
     gene            5864..6844
                     /locus_tag="PLY93_02735"
     CDS             5864..6844
                     /locus_tag="PLY93_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68423.1"
                     /translation="MQKKRGLYVVVSDDFRYLLRIENTYKKANLTPWLRRKDTATLEI
                     PHRLKKNTPVPIDIHHMRNFITCARKGLAGDGVEVRIHAHLRRFIVHASHQGAIAVRV
                     VETLGELKALLTRFKLKYQNIRVALIDRDFTNIVAIFRFRLGLSLFFELERLFDLRLP
                     RVRTIREVLVLTNLAHGGIDALQTNLPFWSKGILGFRFRHVYGNLDKRRVEEMITQKQ
                     WDLLLYRGHAVVEKGCIHWRLADGNFKVPELNTLLYFHLSCLPNPEKLRLDKIPATQL
                     LTTLAVVEDFDDALLVDNFLTRYKLSMNIRSAMRSLQQEYPQFVYFSLSA"
     gene            6981..7646
                     /locus_tag="PLY93_02740"
     CDS             6981..7646
                     /locus_tag="PLY93_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRP68424.1"
                     /translation="MSPTNKPYIAFFDLDETLLSENSGRIFWNYCFKNGYYTPSENMF
                     LGLSLLKFILGVNDTEDFVRGWAKVFNGWSEDKMLALAEKVYHEAIQQTIRPLAVQEI
                     KKHQDNAAQVVILSASTPYVCNPVAQALNVNTVICTRLEIQEGTFTGNFASPYVFGEQ
                     KYLSALQYANEKGIDLQSCYYYGDAHTDRFVMEAVGLPVCVTPDKKLRTHAQEKNWPI
                     LMW"
     gene            7686..8195
                     /locus_tag="PLY93_02745"
     CDS             7686..8195
                     /locus_tag="PLY93_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRP68425.1"
                     /translation="MITGEDNNATYSFSEPFEKRFLELETKFPQRSSLVLWSLHLVQE
                     DIGYVSQAAIDYVAKRIGVAPSWIKGVLSFYSMFREKPIGKYHLNVCSNLMCQLKGSD
                     EIEACIHKKLGIKDGETTPDGKFTYTRQTECLAACGFAPAMLVNNDFYELLTPESIAA
                     LIDELSSKD"
BASE COUNT         2309 a         1996 c         1722 g         2122 t
ORIGIN      
        1 gatcgcgaaa aattaccgca gtgaatttat ttaagaaaat ctttaactca agccgagagc
       61 tttttgtcgc aagcgatccc atgctcgccg atttaggcca cgaaggcgag ttcatcatgg
      121 cacgcattcg ttttattctt atccttttag tcgctgttat tccgctgaaa acgattcttt
      181 ttgaagaatc gagctttgag aactgggtgg gtgtgattgt cgcgctcatt gcgcttttgt
      241 tcgccaccct cattctaatc ggtgtccgca aaaaggtgcc atcgcgctct atctcgtgga
      301 tttcaacaca aaccgacgtg ttgctcgtca cctctgccga ggttggtttt ttactctccc
      361 ctacccccat catcgccagc aacaatatgg tggcatttgg tctttatctc atccnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaagctca cgtatgcagc gggattgatg
      481 gcgctactcc aatacggcat cgttatcttt attgcctcgc gtaccaccgt tggcatgatg
      541 gaccctattt acggttcttt ttattgggat aaccaaatta gtcgtttcct tctcattgca
      601 ttggtgacgc tcttgaccgc cgccattgtg acacgtaacc gtaactattg gcgctcgtcg
      661 atgcacgata aactcacggg attgcataac cggcggtttt tcgatgaatt tttagaatat
      721 aaaattgcag aatgtgcccg ctataaaaga acatttgcgt taattttttt agacgtcgat
      781 tatttcaaga gcatcaacga cacgtacggc catagtaaag gagacgaaat tttatccaaa
      841 gtcggtcacg cgactccgat ttggtcgcgc gctatggcgg cgaagagttt accatcattt
      901 tatcgaacac aaaccacact aaaatccaaa agcggttgaa gcaattctcc ggcattttat
      961 ataaagtcga ctctgcaata aaaatatccg caaccatggg gattgctttt tttcccaacg
     1021 atgcaaccgc agggataaat ttaatacaaa tagccgatga acgcatgtac agcggcaaaa
     1081 aaaatggtag agactgcatc gtcgtaaatt agaatgccga ggagaaagtc tacattcaga
     1141 aaaaacgaca aacaactatg gagaaaaacg agcgcatttt aagtttagaa gacgatgtcg
     1201 agtacgcccg cgtactctcc caagcgctgc gtatttttgg cgaggtgcag ttcaccgcat
     1261 accccgaccg ttttttatcg ctcatcgaaa cctttaagcc gactctcctt atttctgact
     1321 acaacttaaa ccaccccacc ctcaacggta tcaaagtgac ccgcatggtg cgtcgcgatg
     1381 ctaactcaaa ctttttaccg attttattgc tttcgggcga agaggatatg gaagtcatcg
     1441 aagaagcgta taagtcgggg attgacgact atgttttaaa gccagttatt ccccgtttcc
     1501 tcatcgccaa aatcgagaac cttctttttc aaacgcgtaa aaaactgaat gcacaagcgt
     1561 tgtctggact tcccggtaac tcggcgattg aaactgagtt ctatttacgg tggcaaagta
     1621 aaaaggcctt taccgccgcg tataccgatc tcgacaattt taaacctttt aacgacgaaa
     1681 aaggcgtcaa gaaaggcgac gaggcaatcc aaattgtttc tgaaatttta taccaactgc
     1741 gcgccgagac tgaacgccac cgcctttttg taggccatat cggaggcgac gatttttttc
     1801 tcatgggggg attaacctcg ataacgagcg ccgtcaaaaa attgcacaag cgttttgccc
     1861 tcgaaagccg ccgctttttt tccgcacctg aattaaaatc tggtttttat cgaggcaccg
     1921 accgccaagg aaatttttgt tcgtttccat tgctaacgat ttccaccgct ctcatcaaga
     1981 atatttcgta caccactctc ggaaatttta cccaacttac tgagttggcg gcacgcgcaa
     2041 agaaagcggc caaaaaatcg acaaacaaat ttgtaattta cgatgtcgct gtgctctctg
     2101 tgccccaaaa agtacaaaac ttatttgaac ttcaacaaaa caaaggccaa aaaaaacagc
     2161 aattccgccg acaaaaattg aaataatgct ctctcccctg tgtcgatttt taatacgtaa
     2221 caattcttat cgttaggtgt ttatgcaata tgttttagat cttttggtgt atgtggtact
     2281 cggcgctgta ttgagttggt ttttctatta tttgaagcgt ctggatattt ttggtggctt
     2341 tttgggcggc gccctcgttg cagtcattgg ggccatttta ggcgcatttt tgctacaagt
     2401 gccgctaaaa ttcctcattg atgcgttaca aaatggattt tatatctcca acgtgaacgt
     2461 gatcgccgcg ctcatcggtg gcgttgcgag cctttggctt ttaagtaaaa tcaataacgg
     2521 gcgtaagcgc aaagactatt gaaaagaata ggcgtgttca tcgcatgcgg catccttggt
     2581 tgtgtgccgt caaaattaac ccaccccgat ttaacgcgct attttgcaaa agccgaatac
     2641 atcccctcaa agggaatcgg cgccgacggc tgggcgtacg ctcgtcgtcg acggcttttg
     2701 aaacggtatt tacaagcgtt acatatcgac tttatcgaaa aagatcgggt agcgccaacg
     2761 ccgcaaaaca aataccgcga tattgttctc gccgttagcg gtaaaggcga taaaaaaatt
     2821 atggtaacct tagcgctcca agcgcacacc agtgggaatc ccaccgatga gtttataaaa
     2881 gcacagcacg aatgcgacac ttatttattt acatttttat cgacgctgga acaacgttta
     2941 ttgcgttggg agagtattga atttacacag acagaaacct gcaaccgttt gacagcgcat
     3001 gacggacacg aaaagtaaac tatgaaagag attgtcatcg ccgccccccg aggtttttgt
     3061 gctggcgttg accgcgccat ttccatcgtc gagcgtgctc tcgaagtctt tggcaccccg
     3121 ctttacgttc accacgaaat tgtacataac cgttatgtcg tcgattcttt gcgcgccaaa
     3181 ggggtcgttt ttgtagacgc tatcgatgaa attcccccca accaaaatgt tatttttagc
     3241 gcgcatgggg tctcgccagc ggtcactgca gcggccaaag aaaaaaattt aaaaattctg
     3301 gatgccacgt gcccgcttgt gactaaagtg catatcgaag cgcgtcgata cgcgcgcgat
     3361 ggttatcaaa ttgtcctcat cggtcataag catcacatcg aagcgcgcgg cacatacggc
     3421 gaggcccccg aacaaatcac cattgttgaa acattagcag acatcgacgc actcaatttc
     3481 tctcccgatg caaaactggc gtacattacc caaacgacgc tttccgtacg cgatactaaa
     3541 gggttaatcg aacgcctcaa agagcgcttt ccccaaatcg aaggtccaag ttcgagcgac
     3601 atttgttacg ccaccaccaa ccggcaagaa gcggtgtcgg tgattgcccc cgaggcagac
     3661 attatgttcg tcatcggctc caaaaattcg agcaactcca accgcctcaa agaaatcgcc
     3721 gaagcacagg gcacaaaatc ttatctcatc gataacgccg acgatctaaa gcccgaatac
     3781 ttcaccgatg cagtacaaaa aatcggcatt accgccggcg cttctgcccc agaaattttg
     3841 gtaaaacaag ttactgatcg gctgcgcaat gaatttggct tcaccggcat taaagaggtg
     3901 cgccttaaaa acgaagacgt gactttcaaa ttgcccgtcg aattacgggc aacatgaagc
     3961 gctacttttt gttttatatt ttcttcttca ctcccctcgt tgcagaagtt tttgacgaag
     4021 ttaaaagcaa taccaaagcc ggcattgcag cataccaaac gcgccgcttg gccgaagcgc
     4081 gccaattttt atccacggcg ctcaacatcg attcgacgtg gatgcctgct gttttatacc
     4141 atggtgaagc atttgccgat tatttaaccg ccgaacttta tatccacaac actcaaaaat
     4201 atatccactt tgaaaatttc ccaaacgccg aaagcgcgct cgacgaggcc gaagggtatt
     4261 accccgcaca cccacaaatt cccattttac gagagaaaat ccgcaaggca cgagaagcca
     4321 atactaaaat tatattggca aaactacctg aggcgaaacg taagaaatat gaagcatcga
     4381 tggaagcggc aaagctggcg atgaaccaaa gtaaatacgc cgaagcgatg caccactaca
     4441 gtcttgcgct caaaatagct cctcagtcgc tcgacgcaca aatgggctat gctgaggcag
     4501 agcgtttttt caaacaagac ggcaaagaag aaaaaattag cactctcttt cagcaagcag
     4561 aaaagcacaa aaaagaacgc cgcttcgcgc aagctgtgag taattatgac cgcattttac
     4621 gcctcgaatc caatnnnnnn nnnnataata aagaagcgct cactaacaaa gccgcgctcg
     4681 tcgacctcat gcagcaacaa ctcaatgctg aagaaagaaa aaatttggct aaagagtatt
     4741 tagtgagcgg taacgatgcc ttcaaacggc atgacttcgc cttcgccatc gaacaatacc
     4801 gcctcggtaa agcgatggat ccccgcattg ccgactggga cgcactctta aaaaaagcaa
     4861 tcgctgcgaa gaaaaagcaa gatgaaaact tatttgtcga taaagtaaaa gagttagaac
     4921 aacgctacca agcagcaacg ctgcaaatgt tgctggaaaa ttttgctttg gcggtagaaa
     4981 atttagaagc agtcatcgaa attgcaaaag aattgaaaca aaatgaaacc caaaagcaag
     5041 cagaagaact cttggtcatt gcaaaacaag cgatgctgcg gcaagatcag gaatacatca
     5101 cccgcgactc accgtactat tcttttgtgc aatcactcac tgctctggga ttgaacagct
     5161 atacgaacaa agattgcagc gccaccctca aacactttgg cgcaatcgtt caaattttcc
     5221 ccaaacaccg tgtttctaat cagcacatca tgtcttgcac gattattcta caccccgagc
     5281 gcaaagaaag tattattcaa gaactcattg aaagtattta ccgcatgaaa gacgcaaatc
     5341 cttttgaagc acgtcgatat ttcgatattc taaaatttat cgatcccaaa aatcccaaaa
     5401 tccccgaact cgagcgggag cttgccgaga aaactcaagt gctcaaaaag tcggtgaaga
     5461 gcgccgaata tatcgacgcg ttgtaccgcc gtgctttggt cttatcgcaa agcgaccccc
     5521 gcgcggctct cgatatatta cgccaacttc tcgcagaaga tcctgccaac ggtaaagcgc
     5581 gcgcactttt tgcccgcatc gaagcgcgca ttaaacgaca ggaatgggcg gattcggata
     5641 gacccgtcgc tcccgaagcg cttaaagcgt acgccgacgg tattgtttac tataacaccg
     5701 gccaaatttt gcaagccaaa gcggcgtttt cacaagcgct taatttagcc cccaattttg
     5761 aacgcgctca agttgcgctc aaaaagtgcg aatcgtactc gaaaggagcg cgtttttgag
     5821 atactttctt gcctgcgaga cggtgtttca gaaaacactt ccattgcaaa aaaagcgcgg
     5881 gctctatgtc gtggtaagcg acgattttcg ttatttactg cgcatcgaga atacgtacaa
     5941 gaaagcaaac ctcacgccgt ggcttcgtcg taaagacacg gctacactcg agatacctca
     6001 tcgtctaaaa aaaaataccc ctgtacctat cgacatccac cacatgcgta attttattac
     6061 ctgtgcacgt aaaggtcttg ccggtgacgg cgtcgaagtg cgcatccacg cacacctacg
     6121 ccgctttatt gttcacgctt cgcaccaagg cgctattgcc gtgcgagttg tcgaaacctt
     6181 gggagaactg aaagcgctgt tgacgcgctt caagctcaaa taccaaaaca tccgcgtcgc
     6241 tctcatcgat agggatttta caaacatcgt cgccattttt cgctttcgtc ttggtcttag
     6301 tttgtttttt gaactcgaac gtcttttcga tttgcgcttg cctcgtgtgc gtaccatacg
     6361 cgaagtcttg gtgctcacaa atttagcgca cggtggcatc gatgcgttac aaaccaattt
     6421 gccattttgg tcgaaaggta ttttaggatt tcgtttcaga cacgtttatg gcaacctcga
     6481 taaaaggcgc gtcgaggaaa tgataacaca gaaacagtgg gatttactcc tctaccgagg
     6541 acacgccgtc gtggaaaagg gatgtatcca ctggcgtctg gccgacggta attttaaggt
     6601 acccgaactc aacaccctgc tctattttca tttgtcgtgt ttaccaaatc cagaaaaact
     6661 gcgtctcgac aaaataccag ccactcaact tttaaccaca ctcgctgtcg ttgaagattt
     6721 tgacgatgca ctgttggttg acaatttttt aacgcgttat aaattatcga tgaacatccg
     6781 ctcggcgatg cggtcactgc aacaagaata tccacaattt gtttattttt ctctttccgc
     6841 ttaagagcct gtgcaccaac gcgctcagac agttctttta cacgctgcac gagttggcgc
     6901 acaatctctt caatgttcaa ttttacagtt gtcgcacaga ctggcgatta tccttataac
     6961 catagctgaa taatttatat atgagcccta ctaacaaacc ttatatcgcc tttttcgatt
     7021 tagacgagac cttgctctca gaaaatagtg gtcgtatttt ttggaattac tgtttcaaaa
     7081 acggctacta caccccctcc gaaaatatgt ttttgggcct gtctctgctt aaatttattt
     7141 tgggcgtgaa cgataccgaa gactttgtgc gcggctgggc aaaggttttt aacggctgga
     7201 gcgaagacaa aatgcttgca ctggctgaga aagtatacca cgaagcaatc caacaaacca
     7261 tccgtcctct tgcggtacaa gaaatcaaaa aacaccaaga taatgccgcg caagtggtta
     7321 ttctgtcggc gtcaacgcct tacgtgtgta atcccgtagc gcaagctctc aacgtcaata
     7381 cagttatttg cactcgttta gaaattcaag agggcacatt cacaggtaat tttgcgagtc
     7441 cctacgtttt cggcgaacag aagtatttat cggctttgca gtacgccaac gaaaagggca
     7501 tcgatttgca atcgtgctat tattacggcg acgcacacac cgatcgcttt gtcatggaag
     7561 cggtcggttt accggtttgt gtaacccccg acaaaaaact acgcactcac gcgcaggaga
     7621 aaaactggcc tatcttaatg tggtaattgc tcgtcaccgt aacacgtttt ttatttcagt
     7681 cgactatgat aaccggcgaa gataacaacg ccacgtatag tttctcagaa ccttttgaaa
     7741 aaagatttct tgaacttgaa acgaaattcc ctcaacgctc ttctcttgtg ctgtggtcgc
     7801 tccacttagt acaagaagat attggatatg tctcacaagc agctatcgat tatgtggcta
     7861 aacgtatcgg tgtcgctcca tcttggatta aaggggttct ttctttctat tcgatgttcc
     7921 gcgaaaaacc aattggtaaa taccatttga atgtttgttc caatttaatg tgccagctca
     7981 aaggttctga tgaaattgag gcgtgtatcc acaaaaaatt gggtattaaa gacggtgaaa
     8041 caactccaga cggtaaattt acttataccc gccaaaccga atgcctcgct gcttgtggtt
     8101 ttgcgccggc aatgctcgtc aacaatgact tttacgaact cttgaccccc gaatcgatcg
     8161 cagcgctcat tgatgaactt agttcaaaag attgaca
//
LOCUS       DAOMPD010000048         8163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3792_length_8163_cov_2.480266, whole genome shotgun sequence.
ACCESSION   DAOMPD010000048 DAOMPD010000000
VERSION     DAOMPD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3792_length_8163_cov_2.480266"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLY93_02750"
     CDS             complement(<1..985)
                     /locus_tag="PLY93_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRP68426.1"
                     /translation="MEVYESEGGYTQLKRLLRDEKEKWTPNAIIDEVKKSNLRGRGGA
                     GFPTGMKWSFVPKDGPEPKYVVCNADESEPGTFKDRFMVEEIPHSIIEGMVIAALALN
                     SEQGYIYLRGEFYKGWERIEAAIKEAYAAGYLGDNILGTGRKFHLATYRGAGAYICGE
                     ETALLESLEGKRGHPRLKPPFPAVKGLYASPTVVNNVETFATVPSVFRLGAEAYSKLC
                     YAGNEKSGGTKIFSISGHVKNPGVYELPLGIPLLEVIQIAGGLLDGRTLKAVIPGGSS
                     TPILTPDEVAKAVLDYEGIAAVGSMLGSGGIMVLDNTTDLVELTYRVAHFYG"
     gene            complement(1198..2031)
                     /locus_tag="PLY93_02755"
     CDS             complement(1198..2031)
                     /locus_tag="PLY93_02755"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRP68427.1"
                     /translation="MIESITPQNIEAAANYLRTKITFPLQVGVVLGSGLSQFASEAGK
                     SVAIEYAKVPHFPQSTVEGHSGSFVVGFIEEASVMLMQGRVHFYEGYSMQQTAMGIRL
                     MASLGVRTLIVTNAAGAVNANFAPGDFMLIDDHLNLMGTNPLIGKHYSDWGDRFVDQT
                     DSYTPALREIALRVAAQNNLQLQRGVYAALSGPTYETPAEIRMLRTLGADACGMSTVP
                     EVIVARQSGMRVLGLSLITNMGAGMASSLNHTEVVEIAQRRKVDFQRFISGIIRAINA
                     E"
     assembly_gap    2220..2233
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2234..2812)
                     /locus_tag="PLY93_02760"
     CDS             complement(<2234..2812)
                     /locus_tag="PLY93_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68428.1"
                     /translation="MNWQTLKNKFLRSRSKDAVGVITPMKKTLIDRFLAWFSISRNRF
                     RFIVLLFVLDFVAFMSLTRSSYLQLLNPIAFLSVSKDIGMETMELYFPRSLSLTGIEK
                     IYSNTDDAPTIKIQNDPQGNPADDVIDQTKVLDDAQVKAEVIAIQKKVAHPIHEIDAL
                     NLTANEARARRVMYELIAGPSGDLDTLKARNLL"
     gene            complement(2787..3971)
                     /locus_tag="PLY93_02765"
     CDS             complement(2787..3971)
                     /locus_tag="PLY93_02765"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRP68429.1"
                     /translation="MGLVHRLVKVKILAIFYPQRGQKITIFTKIILVSLISLLGGALS
                     AEQIRSTLVYIEGEAYLSAGNLKRLHNNLSVHYNRNEKQGEVRFGKRIILFTLDKADY
                     QVQAEKHELDAVGVLDRKGLFFSQEFVEEILTELNLPVSYRFSKTHLFVEATEKRVSA
                     EKLDFIYIDAGHGGKDSGALGYFDVAEKDITLRLARKLYKQLKIDFPQTKVYLTRSRD
                     KFLSLEKRSQLANRQLRKKKDAPSFGLFVSIHCNSHLSPRVKGFEIYYLAQNSRNKNL
                     RELYLRENNRYEKSAYIRRLTSRLMNAQIQRESKNLARSVFLGIGNRLDGMIKSRKVK
                     KADFAVLRGSLMPAILIETGYLTNKDDLKKLRSAAYADAFVVGVSHGIGAFLRELANT
                     EK"
     gene            4035..4715
                     /locus_tag="PLY93_02770"
     CDS             4035..4715
                     /locus_tag="PLY93_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68430.1"
                     /translation="MRLLKKEHPTECIDPYLQFVVNTYVYRSRETYKNYFEQENTQPL
                     ADYFFEKIYTLDGKSERDALAEKTYNRFKGMLTEKSRGRIENLLLLNEITDRLDREMA
                     DFLRHHSKYLLSDIRTPHIDLDKLPLIYRKCNRMEDRVEQLRLVVQNLESFFELSKHP
                     LAGVIMRPVSLAARTVGFTSLYKIFEAGYDATKPISKEVFFEFTQYVRKRETQFLEKI
                     YKKRIHLP"
     gene            4712..5323
                     /locus_tag="PLY93_02775"
     CDS             4712..5323
                     /locus_tag="PLY93_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68431.1"
                     /translation="MIKIPIKSKKRDKAGFTHLVLAKPNNYTHKLGQYVYLKVSETQT
                     KALAIASHPSEPQLLFLFRGEFEHSIVPHLSQPQGDGFRCVLDKPQRMLFITHGTGIT
                     ALRGIIIERKRLGFTHDTLLYGSRHADEEPELDCLQADFGVKQLRAYSSPPEELHLQD
                     ILRGFDVDNFDAVLLAGSTKMLSSCRQILYSMKYSANKIFTNY"
     gene            complement(5329..6857)
                     /locus_tag="PLY93_02780"
                     /pseudo
     CDS             complement(5329..6857)
                     /locus_tag="PLY93_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
     gene            7004..7669
                     /locus_tag="PLY93_02785"
     CDS             7004..7669
                     /locus_tag="PLY93_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRP68432.1"
                     /translation="MPFGEQKLNLYFSGELYFSILPHMKKKTTNENTSDNLTGVVAEN
                     VKRLRSDMDLSLDKLASLSGVSKAMLSQIEQARSAPSINVLWKIARALDVPFAALISK
                     RNDASQQVMRADDMKTLSSNKGKFLSKALFPLDVSRRVEFYELTVKVGCVEKAVPHPP
                     GTSENLVMAQGSMEVDIDGAKNMLYEGDAIYFIADVPHVYRNDSNKDAIAYLVMTYAE
                     KRV"
     gene            7733..>8163
                     /locus_tag="PLY93_02790"
     CDS             7733..>8163
                     /locus_tag="PLY93_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRP68433.1"
                     /translation="MSQIKNIETLGVHAGQVVDPTTGSRAVPLYQTTSFVFNDTDHAA
                     NLFALKEFGNIYTRIMNPTTDVFEKRIAAIEGGVAALATASGMSASALAVLNLSRPGD
                     EIVSYDNLYGGTYQLFHHTIQLLGRTVKFVKSGDLDALKKA"
BASE COUNT         2136 a         2058 c         1817 g         2138 t
ORIGIN      
        1 gaccgtaaaa gtgcgccact cggtatgtga gttccactaa atcggtagtg ttgtcgagca
       61 ccatgatccc tcccgacccc aacatggagc ccacagcggc aatcccttcg taatcgagca
      121 ctgcctttgc aacttcatcg ggggtgagga tgggtgtgga actgcctcca ggaatcaccg
      181 ctttcaacgt ccgcccgtcg agtaatcccc ccgcaatttg gatgacttcc aaaaggggaa
      241 ttcccaacgg taattcgtac acacccgggt ttttcacatg gcctgaaatc gaaaaaattt
      301 tagtgccgcc cgatttttcg ttgcctgcat aacagagctt ggagtatgcc tctgcaccca
      361 agcggaaaac cgacggcacg gtggcgaatg tctccacgtt gttcaccaca gtcggtgaag
      421 cataaagacc tttcaccgca gggaaaggcg gcttcaaacg cggatggcct cttttccctt
      481 ctaaactttc gaggagagcg gtttcttcgc cgcagatata tgcaccagcg ccacggtagg
      541 tcgcaaggtg aaatttgcga ccggtgccta aaatgttatc gcctaaatat cctgcagcat
      601 acgcttcttt aatcgccgct tcgatgcgct cccatccttt ataaaattca ccgcgcaagt
      661 agatataacc ctgttcgctg ttgagcgcga gtgcagcaat caccatgcct tcgataatcg
      721 aatgtggaat ttcttcgacc atgaatcggt ctttgaatgt tcctggttca gattcatcgg
      781 cgttgcagac cacgtactta ggttcggggc catctttagg cacaaacgac catttcatgc
      841 ccgtgggaaa tccggcgccg ccacggcctc ttaaattcga cttcttcact tcatcgatga
      901 tggcgttcgg tgtccacttt tctttttcgt cgcggagaag ccgtttgagt tgggtgtatc
      961 ccccttcgga ttcgtagacc tccatcgtgt gcgagttttc tttgtggaaa taactcaaca
     1021 caacgcggtt ttctttttcg tgcggggggc gggctttagc agggtgcata tagttgcatc
     1081 tttagatgcg cgcaaagggc gtaaatgaaa aagaaaaatt tgcctctttg gagcctacgc
     1141 gagatggcgc acaggctctt agccacaacg ccgtgtcagc tcgttctgtg gtcgatatta
     1201 ctccgcatta atcgcgcgga taatgccaga gataaatcgt tgaaaatcga ctttgcgtct
     1261 ttgggcgatt tctaccacct ctgtgtggtt gagtgaagat gccatacctg cccccatatt
     1321 ggtgatgagg cttaatccta agacacgcat tccactttga cgcgccacaa tcacctcggg
     1381 tacggtgctc atgccgcacg catctgcccc cagtgtacgc agcattcgga tttccgccgg
     1441 cgtttcatag gtcggcccgg aaagtgcagc gtacacccct ctttgcaact gaagattgtt
     1501 ctgggctgca acacgcaaag caatttcgcg taacgccggt gtgtacgaat cggtctgatc
     1561 gacgaaacga tccccccaat cgctataatg tttaccgatt aaagggtttg ttcccattaa
     1621 atttaggtgg tcgtcgataa gcataaaatc accgggagca aaattagcat tcaccgctcc
     1681 cgctgcgttg gtgacaatga gcgtacgcac acccaaagaa gccatgagac ggatacccat
     1741 cgcggtttgt tgcatcgaat aaccttcata aaaatgcaca cggccttgca tgagcatcac
     1801 cgacgcttct tcgataaagc cgacgacaaa actgccgctg tgcccttcga cggtggactg
     1861 tggaaaatgc ggtaccttag cgtactcaat cgcaacactt tttcccgctt ccgacgcaaa
     1921 ctgcgaaaga cccgaaccta aaacaacgcc gacctgcaaa gggaacgtaa ttttagtacg
     1981 caaataattt gcagcggctt cgatattttg cggagtaatc gattctatca tgatttttgc
     2041 aggcttattc tttaagaagg acaaaatcga caccagctaa atttttcttg agagattcaa
     2101 gaatacaata ttcggctatt ttcctttcat tggggcgcat tttataccat acactctttt
     2161 cggttgtaat ataaagttta ccgtcatggg tccacaacga gcgtaagaac attcgttctn
     2221 nnnnnnnnnn nnnaagtagg ttacgcgctt tgagcgtatc cagatcgccc gatggccccg
     2281 cgatgagttc gtacatcacc cgccgcgcac gcgcttcatt tgcggtgagg tttaatgcgt
     2341 caatttcatg aatagggtgc gctacctttt tttgaatagc aattacctct gcttttactt
     2401 gtgcatcgtc taaaacttta gtttgatcga tgacatcgtc ggcgggattt ccttgcgggt
     2461 cgttttgtat ttttatggtg ggtgcatcgt ctgtattgct ataaattttt tcaatccccg
     2521 tgagcgacaa tgaccgtgga aaatagagtt ccatcgtttc cataccgata tcttttgaaa
     2581 ccgacaaaaa cgcgatcggg tttaagagtt gtaaataact gctacgcgtg agagacatga
     2641 acgcgacaaa gtcgagaaca aaaagaagaa caatgaagcg aaaccgatta cgggagattg
     2701 aaaaccacgc caaaaagcga tctatcaacg tctttttcat tggggtaatt acacccaccg
     2761 cgtctttcga gcgagagcgc aagaatttat ttttcagtgt ttgccagttc acgtaagaaa
     2821 gctccaatac catggctaac gcccacaaca aatgcatcgg cataagccgc gctgcgcagt
     2881 tttttcaaat cgtctttatt tgttaaatat cccgtttcaa tgagaattgc tggcattaaa
     2941 gaacccctga gtacggcaaa atccgctttt ttcactttac gagatttaat catcccgtca
     3001 aggcgattgc ctatacccaa gaagacactc cgtgcaaggt ttttgctctc gcgctggatc
     3061 tgtgcattca tgagacggct agttaagcga cggatatacg cgcttttttc atagcggtta
     3121 ttctcgcgca agtagagctc gcgtaaattt ttattgcggc tattttgtgc taaataataa
     3181 atttcaaacc ctttgacgcg cggcgacagg tgggaattac aatggatact cacaaaaagt
     3241 ccaaacgaag gagcgtcttt ttttttgcgc agttgtctat tcgctaactg actgcgcttt
     3301 tctaaactca gaaacttatc cctcgaacgc gttaaataaa ctttagtttg agggaaatct
     3361 atcttgagtt gcttgtagag ttttcgcgct aaacgtaatg taatatcttt ttccgcaaca
     3421 tcgaagtaac ccaatgcgcc cgaatcttta ccgccatggc cagcgtcgat ataaatgaag
     3481 tcgagctttt ctgcactcac ccgcttttct gtggcttcta caaacaaatg cgttttacta
     3541 aagcggtacg agacaggtag gttcagctca gttaagattt cttcgacaaa ctcctgcgag
     3601 aaaaaaagcc cttttcggtc taaaacccca acggcgtcaa gctcgtgttt ctccgcttgc
     3661 acttggtaat ctgccttgtc gagagtgaaa agaatgattc tttttccaaa gcgtacctcg
     3721 ccttgcttct cgtttcggtt atagtgcacg gacaaattgt tgtgcagccg ctttaagtta
     3781 cccgccgaaa ggtatgcctc tccctcgata tacaccaaag tgctacgtat ttgctcggcg
     3841 ctcaacgctc cccccaaaag cgaaattaaa cttacgagga taattttggt aaaaattgtt
     3901 attttttgcc ctctttgcgg gtaaaatata gcaagtattt taactttcac cagtcgatgt
     3961 acaagcccca ttgaatatgc cctattttgg attgtcctgt gccaccggaa agcagttttg
     4021 actctccaac tcttgtgcga ctactaaaaa aagaacaccc caccgaatgc atcgacccat
     4081 acctccagtt tgtggtcaac acttacgtct accgctcgcg tgaaacgtac aaaaattact
     4141 ttgagcaaga aaatacacaa ccacttgccg attatttttt tgaaaaaatc tacaccctcg
     4201 atggtaaatc cgaacgcgac gcgctcgctg aaaaaacata taatcgtttc aaaggcatgc
     4261 tcaccgaaaa gagccgtggc cgcattgaaa acttgttact cttgaacgag atcaccgacc
     4321 ggctcgatcg cgaaatggca gattttttac gccaccactc gaaatacctt ctcagcgaca
     4381 tacgtacccc acatatcgat ttagataagt tgcccctcat ctaccgcaaa tgcaaccgca
     4441 tggaagaccg cgtcgaacaa ttaagactcg ttgtgcaaaa tttggagtct ttttttgagc
     4501 tttcaaaaca ccccctcgcc ggggtcatca tgcgccccgt ttctttagcg gcacgcacgg
     4561 taggattcac ctcgctctat aaaatttttg aagcgggata cgatgccaca aaacccattt
     4621 caaaagaagt cttttttgag ttcactcaat acgtccgtaa acgcgaaaca caatttttag
     4681 aaaaaatata taagaaacgt atccatttac catgattaaa atccctatta aaagtaaaaa
     4741 acgcgacaaa gcgggtttta ctcatcttgt attagccaag ccgaacaatt atacgcataa
     4801 actgggacaa tatgtatacc taaaggtgag tgagacccaa acaaaggcgc tcgcgattgc
     4861 atcacatccc agcgaaccgc aactgctctt tctctttcga ggtgagtttg agcattccat
     4921 cgtaccgcac ctttcacaac ctcaaggaga tggttttcgg tgtgtgctcg acaaaccgca
     4981 acgcatgctt tttattacgc acggcaccgg catcaccgcg ctgcgtggta taattattga
     5041 aagaaaaaga ttgggattta cacacgacac cttactatac ggctctcggc acgccgatga
     5101 agagcccgaa ctcgattgct tacaggcaga ctttggcgtt aaacaactac gcgcctattc
     5161 aagtccaccc gaagagttac acctacaaga tattctacgt gggtttgatg tcgataactt
     5221 cgatgcagtg ctactcgccg gctcaacaaa aatgttatca agttgccgtc agattttgta
     5281 tagtatgaaa tattctgcca acaagatttt cactaattat tgaaaatctt acgccgctaa
     5341 gcttttcata ccctgaaaaa tgatcttatc gcggttatac cccaacgcct cgggtagcgt
     5401 cttaaattta ttcaacccta ccccaatttc aatttcttca ccggtgaatt gtgcgggatg
     5461 ctcgtagccg catgcatgcg ccaacgtcaa caactctttg cgaaagcctt tgatgtaccg
     5521 cgccgcacgg aaagttttgt ctttcacgtc gatgccgcgt tgcagccacc agttttgcgt
     5581 cgcgataccg gtggggcaat ggccggtgtg gcatttttgc gcttggatgc aaccgatggc
     5641 catcatcggc tcacgtgcta cgttaatgag gtcgcagcca tcgcgagcgc caccaccgcg
     5701 cggtcgggga atcccaattt ccccgaaccg atgaatgcaa tgcgctcaga aattttttct
     5761 ttttgaaaaa tttggtacac acgggcaaag cctgttttga aaggtaacgc aacgtggtcg
     5821 gcaaacgtca acggcgcagc tcccgtcccc ccttcgccgc cgtcgatggt gatgaaatcc
     5881 ggccctttgc ctgtttcacg catgcgcacc gccagctctt gccaaaacgg caattcacca
     5941 atcgcgcttt taatgcccac ggggcgaccg gtggcgctcg cgatgcgttc aatgaatgca
     6001 atcatttcat cgacattcga gaactcactg tgaaaattcg gagagataca atcttgtccc
     6061 ttcggcacgc cgcgaatcgc cgcaatttca gcggtgactt tggccgccgg tagaattcca
     6121 cctttgcccg gttttgcgcc ttgcgagagt ttgatttcaa caatgcgaat ttgtggattg
     6181 tcttcaatct ttttagcaag aatgtcgagg ttgaaacctt tttcatcgcg ggcaccgaaa
     6241 taccctgtgc ctatttgcca gcacacgtcg gcgccttttg catggtattt tgaaaatccg
     6301 ccctcgccgg tgttgtgcca acaattggct tcacgcgcgc cttgattgag cgcagtgacg
     6361 gcgcggtcgc ccaacgaacc gaatgacatc gccgaaatgt tgacaatcga aggaggccgc
     6421 cacgcgtttt tacggttgtg cccttcgccc aaaattttaa gcgcaggtat cgcagtgggg
     6481 tcgttgttga taagaaccgc ttttgattct gggaaaggaa agaccgcatg cttgatgatg
     6541 ggataccccg cttcgtagag ttgctcggtg gtaccaaaac caaaattcgt gttttggcct
     6601 ttggcggtcg catacaccca acgcctttcc gaacgattga aaggcatttc ttctttgtcg
     6661 ttggcaaccc agtactgccg taactcaggc ccaatgactt cgagcatata acgcaaatga
     6721 ccgaccaatg ggaaattccg ctggattgca cgcttacgct ggatcatgtc gcggatagca
     6781 acaaggagaa gaaaaactcc cataacaata aaaatgctcg cgacgatatg ttcttccatc
     6841 caataccaaa ttgccataaa tgacagtcaa taggcaaggg gttgtatgtc aacggaattg
     6901 ttagattttc ctagccaaaa gagtacgatc ctgtgcgtca acttgctgaa acaatttctt
     6961 tgcaaatttt tgtacgcgct gcgcgagttg ccgcacagac tctttgccct tcggcgagca
     7021 aaaactaaat ttatatttct cgggtgagct ttatttcagt atactcccac acatgaagaa
     7081 aaagacaacg aatgaaaata catctgacaa cttaaccggt gttgttgccg aaaacgtgaa
     7141 aaggttgcgc agcgatatgg atttatcgtt agataagctc gcatcgctat cgggggtttc
     7201 aaaagcgatg ctcagccaaa tcgaacaagc gcgtagcgca ccgtcgatta acgtactttg
     7261 gaaaattgcc cgcgcgctcg atgtgccgtt cgccgcactc atctctaagc ggaacgacgc
     7321 ctcgcaacaa gtgatgcgcg ccgacgacat gaaaaccctc tcatcgaata aaggtaagtt
     7381 tctttcaaaa gcgcttttcc cattagacgt gtcacgtcgt gtggagtttt atgaactcac
     7441 cgtcaaagtg ggttgtgtcg agaaggcagt cccccaccca cctggtacat ctgaaaattt
     7501 agtgatggcc caaggctcaa tggaggtgga tatcgatggc gcaaaaaata tgctctatga
     7561 aggcgacgct atttatttta tcgcagatgt accgcatgtg tatcgtaacg atagtaacaa
     7621 agatgcaatc gcatatctgg tgatgacgta tgcagagaaa agagtttaaa aaagatttga
     7681 catcgtgaac aaaatttcga tattgcgaac aaggaaaatt taaggagaat ctatgtcaca
     7741 aataaaaaat attgaaacac tcggagtaca cgctggtcaa gtggtggatc ccacaaccgg
     7801 ctcgcgcgct gttcccctat accaaaccac atcgtttgtg tttaacgaca ccgaccacgc
     7861 ggcgaatctt tttgcgctca aagagttcgg taacatctat acgcgcatca tgaaccccac
     7921 caccgatgtg tttgaaaaac gcatcgcggc gattgaaggc ggcgttgccg cactcgcgac
     7981 ggcatcgggc atgtcggcgt cggcgctcgc ggttcttaac ctctcacgcc cgggcgatga
     8041 aatcgtttcg tacgacaacc tctacggcgg tacgtatcaa cttttccacc acaccattca
     8101 acttttgggg cgcaccgtaa agttcgtaaa atcgggcgac ctcgacgcgc taaaaaaggc
     8161 gat
//
LOCUS       DAOMPD010000049         8106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3832_length_8106_cov_2.882375, whole genome shotgun sequence.
ACCESSION   DAOMPD010000049 DAOMPD010000000
VERSION     DAOMPD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3832_length_8106_cov_2.882375"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLY93_02795"
     CDS             <1..660
                     /locus_tag="PLY93_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP68434.1"
                     /translation="DWRTAFIALGIFSMFYGAFAAYRQKSFRSVIAYSSLSHMGLLLA
                     GSITAHEYALTGSIIQNVGHSLANALLFVLVCMPMVRGKSDTLGEMRAPKSFFYWGAF
                     VIAVFSAIGVPGTIGFVGEFFILFGLSFTSWPITIIAVFTIVISAAYMLRLFHKVRAT
                     EADTSWRPNIIERVCLVLLSASIIYFGVAPSTMGEMGQSAAKTILASPAQPVQQGNNQ
                     R"
     gene            657..2189
                     /locus_tag="PLY93_02800"
     CDS             657..2189
                     /locus_tag="PLY93_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP68435.1"
                     /translation="MNFSAKDIFATLPQLVLVVGALVVLTSQLILKKHRVRIAWQLTV
                     LTLIVTLLLVIFGISAADGKSTLLPAAFTPFDALSAFSGSLRYSSFSANSIVVFLALA
                     LAAAFMMRKTLADADLNFSENYFLLLMSVAGYSYAVCAEDLITFFIALELGSIPIIVL
                     VGMNRKSTANNEASLKYLLLSAFAMAFFLLGIALIYGVSGTVRLRDIQNISPHYMRTQ
                     AMMLGYIFIFVGFFFKIGAFPLHSYIADVYEGSQTMFTGILGSLSKAGGLFLMFKIYS
                     VMHDGFRAYMTPVLLVAAVGSLFYGAFASLGTRNLKRILAYSSISHAGFLLCFLVIPS
                     GSSYGLFASFKQEAAAGFYIYTVGYTTASLLGFGVIAYAEAVHLNKEVLTLDNLAQVF
                     NGKGLGSWSLSIATLSLLGMPPLAGFIGKFFLFKYLALSSNLTLAALAAIATAISLYA
                     YIAIVKPIFFDERSTEDNVQFSFGDAGKVTLVAFMAVVGFFAVFTSFLYNTGITAMQR
                     IF"
     gene            2208..3392
                     /locus_tag="PLY93_02805"
     CDS             2208..3392
                     /locus_tag="PLY93_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRP68436.1"
                     /translation="MFKKLNQRKFRLSTLGVANLTMFVFLIGVALSPFAGTCKRAATA
                     EGATDNPLLKSDTIQMAEKFQEAMRQIYKEVSPAVVLIATTAEVNNPMFNDPMFRRFF
                     QIPEGQGRKQSGLGTGFIISSDGYVVTNTHVVQIAQGAKIDKIIVKLTNGKEYQASIV
                     GSDARSDLALLKINAVKNLKTVHLGDSDKVEVGDFAIAIGNPHGLQSTLTTGVISAIN
                     RDLNHDGLPKLQTDASINPGNSGGPLINIRGEVIGINQMIYSQTGGSIGLGFAIPVNH
                     AKQVIEKLKAGKKIRYGYVGLQVDGEPTDEKLDALGVKGKTGLIINQVTLNSPAFKAG
                     LAAYDFITHVDGKPAENFGVLKSAVTQKGPGETIELKIIRQGKEITVKVKIAEAPVQD
                     AR"
     gene            3405..4181
                     /locus_tag="PLY93_02810"
     CDS             3405..4181
                     /locus_tag="PLY93_02810"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRP68437.1"
                     /translation="MTLTIDVGNSHTVVGFWREHALVHTLRLASDARRTADEWRLMLL
                     QALRENSFSQISKASLCSVVPAARLALNEAVRQLGVTEPLWLSSTTPLNFTFAYPQPQ
                     MLGADRLADMIAARHYFGTNCITIDFGTAVTFSVLTDGEFVGGVIAPGITSSLEALFS
                     ATAKLPQIAFRRTQNAVGQSTVESIEIGAYFGWRGMVREILTEIKRELPEHGKNHCLV
                     ATGGIVENIEFAPDFFDVVDKNLTLKGLYLALQNAKAVHD"
     gene            4184..5389
                     /locus_tag="PLY93_02815"
     CDS             4184..5389
                     /locus_tag="PLY93_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRP68438.1"
                     /translation="MPRFYILDRYLLKQFLPSLSVACVFFTFIILIFFLKETIRHAVE
                     KNLDVWVILELCWYALGWTLTLTIPMAFLLSTILVVGALNADSEIIALRAGGITYPRI
                     LRPFLFVAMAASIFLVWFSNFVVPRFADNMENVRKFILTTDPIAAIQPGQFVNLDRNE
                     SFVRKIYIERAVKDEVGNGETFKNIQVRKIASIGGIRRLSELIIAQEGRKILKKTIQG
                     NWIKALRLTKGYLFATSSDGTFQRIDFSNGMLDLNIQERSQVRQMKRIDDMRTLTLPE
                     LINSYEITKDYPNAKKLILKADLELHKRFALSLSTILFVLMGFPLAVVNRRSGKGMGL
                     GISVVFIFGYFAIFLSAEMVSVDRTLLSPFVAAHAANALLFLGALVFILRRLKEISLR
                     RLFGFGRVK"
     gene            complement(5396..6463)
                     /locus_tag="PLY93_02820"
     CDS             complement(5396..6463)
                     /locus_tag="PLY93_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP68439.1"
                     /translation="MATSSLADSQFFIFGESKIVADALEGYLTARKASVHFIHHFDEI
                     KTSDETDNILIAHYNEVKNLAEKLGQLKKKYASFRILLLHEFIDRGKISQPILSITDH
                     LLEKPFSRLSLDKALEQFRFKPLTGKKVLSLSWGGDTLTAKLLATLGATVYNTLPKSS
                     EAASVELIVITPIELGDSFRADLSAIREVYADVPIFMIYDPEAPGVLNSTILNEVAYL
                     LQQPVQRQVLRAKILDFFEQPQRDRRKNPRKKNINQVWISAYNAELKTPELFESPALI
                     DVSQSGLSFQSYTEYRENQAMTVWVVAEDNPDKILDLRAVIVWRRKDGNDDCFKYGIE
                     FTKENPQVYDIFARMIAMHLG"
     gene            complement(6494..6868)
                     /locus_tag="PLY93_02825"
     CDS             complement(6494..6868)
                     /locus_tag="PLY93_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68440.1"
                     /translation="MSEKKKILIVDDSGYMRQVIRRHLESQGFEIVGEATDGTQVVSM
                     AEELKPDLITMDLSMKNLGGIDATKLVKQAIPDAKVIVVSALGESRFIKEAVAAGAHD
                     FIIKPFTEERLLSSVRSALKMN"
     gene            complement(6896..7174)
                     /locus_tag="PLY93_02830"
     CDS             complement(6896..7174)
                     /locus_tag="PLY93_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68441.1"
                     /translation="MKRKEAAFMWQKIKTYFVAHVIGLVLILAGWAFSMNVVAINRFQ
                     MFSVPGGGVSERPIFQSWTFFGLALIIIGAYFPEMWIAMRNRKVNPPK"
     gene            7353..>8106
                     /locus_tag="PLY93_02835"
     CDS             7353..>8106
                     /locus_tag="PLY93_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP68442.1"
                     /translation="MATINEQDIRAKIAKRIVSAGARFAFNERTPLPTLRWIIDQIRF
                     IERLAPDVELTEGVVDLIEYDFFTPATFQPGRILLYSHGGAYTMFSHRTHRSLVSRLA
                     LEFNAQAIAHNYRLAPEHPFPAAVDDTIRIYQYLLSLGYAPANIIAAGDSAGGGLTFA
                     FMLAARDKGLPLPRFAIGISPWVDLTAVGDSMQENKATDVMLSPEGIKKFGLRYVQDV
                     NKLKHPYASPLFADLRGLPSVFLQAAGDEILRD"
BASE COUNT         1937 a         1891 c         1965 g         2313 t
ORIGIN      
        1 gactggcgta ccgcgtttat cgctttgggc atcttttcga tgttttatgg cgcgtttgcc
       61 gcgtaccgac aaaaatcgtt tcgctcggtc atcgcgtatt cgtcgctttc gcacatgggg
      121 cttttgctcg cgggcagcat caccgcgcac gagtacgcac tcacaggatc catcatccaa
      181 aacgtggggc attcgctcgc gaacgcgttg ctttttgttt tagtgtgtat gccaatggta
      241 cgtggtaaat ctgacacatt gggtgagatg cgcgccccca aaagtttttt ctattgggga
      301 gcgtttgtga ttgccgtttt ctcggcgatt ggcgttccag gaaccatcgg ctttgtgggt
      361 gaatttttta tcctgttcgg tctttcattc acatcgtggc ccatcacgat tattgctgtt
      421 tttacgattg ttatcagcgc cgcgtatatg ttgcgccttt tccacaaagt gcgcgccacc
      481 gaagccgata cttcgtggcg gcctaatatc atcgagcgcg tttgtttggt gcttttaagc
      541 gcgtcgatta tttattttgg cgtggcgcca tcgacaatgg gtgaaatggg acagagcgca
      601 gccaaaacta ttttagcctc acccgcacaa ccggtgcagc aagggaataa ccaacgatga
      661 atttttcagc gaaagatatt tttgcaactc tgccgcagtt agtgttggtg gtgggcgcgc
      721 ttgttgtcct aacgagccaa ctaattctta aaaaacaccg cgtacgcatt gcgtggcagt
      781 tgaccgtgtt gacgttgata gtaacgctgc tgcttgtcat tttcggcatc agcgccgccg
      841 acggcaagtc tacgctttta ccggcggcgt tcactccatt cgacgcgttg agtgcttttt
      901 cgggttcgtt gcgttattcg tcgtttagcg caaacagcat tgttgtgttt ttagcgcttg
      961 cgttggctgc ggcgttcatg atgcgtaaga cgctggcaga cgccgattta aatttttcgg
     1021 aaaattattt tctactcctc atgagcgttg ctgggtatag ttacgcggtg tgcgccgagg
     1081 atttaattac cttttttatc gcgctcgaat tgggttcaat ccccattatt gtgttggtgg
     1141 gcatgaaccg caaaagcacg gcgaataacg aagcgtcgct caaatacttg ttgctttcgg
     1201 cgtttgcgat ggcgttcttt cttttaggga ttgcgctcat ttatggcgtc agcgggacag
     1261 tccgattgcg cgacattcaa aacattagtc cgcactatat gcgcactcaa gcgatgatgt
     1321 tggggtatat ttttattttc gtcggtttct tttttaagat tggcgcattc ccgttgcaca
     1381 gttatatcgc cgacgtgtac gaaggctctc aaacgatgtt caccggtatt ttgggttcgc
     1441 tttccaaagc gggcggattg tttttgatgt tcaaaatata ttccgtgatg cacgatggtt
     1501 ttcgtgcata catgacgccg gtgcttttgg tggccgccgt gggttcgctt ttttatgggg
     1561 cgttcgcttc gttgggtacg cgcaacttga agcgtattct tgcgtattcg tcgattagcc
     1621 atgcgggatt tttgctttgc tttctagtta taccctcggg ctcgagctat ggccttttcg
     1681 caagttttaa gcaagaagcg gcggctgggt tttatattta tacggtaggt tacaccacag
     1741 cttcgctttt aggctttggc gttatcgcgt acgccgaagc ggtgcatctc aacaaagaag
     1801 ttttaacgtt ggataacttg gcgcaagttt ttaacggcaa gggattgggg agctggtcgc
     1861 tttcgattgc aaccttatct cttttaggga tgccgccttt agcggggttt attggtaaat
     1921 tttttctctt taagtacttg gcgctttcaa gcaatctcac gcttgcagcg ttggccgcga
     1981 ttgccaccgc tatatcgctc tatgcgtaca ttgcgatcgt aaagccaatt ttcttcgacg
     2041 agcgcagcac agaagataac gttcaatttt cttttggcga cgcgggcaaa gtaacgttgg
     2101 tagcgtttat ggctgtcgtc ggtttttttg ccgtgttcac ttcgttttta tataacacag
     2161 gaatcactgc gatgcagcgg atcttttgac acacactgga ggctactatg ttcaaaaaac
     2221 tcaatcaacg taagtttcgt ctttcgacgc tcggcgttgc caacctgacg atgttcgtct
     2281 ttttaatagg cgttgcgcta tcgccatttg ccggtacgtg taagcgcgcc gctaccgccg
     2341 agggtgcgac cgacaatcct ctcttaaaat cagacacgat tcaaatggcc gaaaaatttc
     2401 aagaagcgat gcgccaaatt tataaagagg tgagtcccgc agtggtgttg atcgccacca
     2461 ccgctgaggt gaataatcct atgttcaacg atcccatgtt ccgtcgtttt tttcaaatcc
     2521 cagagggaca agggcgtaag caaagcggtt tgggtaccgg ctttattatt tcaagcgacg
     2581 gttatgtggt gacgaacacg cacgttgtgc aaattgcaca aggggcaaaa atcgataaaa
     2641 taattgtgaa gcttacaaac ggcaaggaat accaagcatc gattgtcggc tctgatgcgc
     2701 ggtcagattt agctctcctg aaaatcaatg ccgtcaaaaa tttgaaaaca gtccacctcg
     2761 gcgattccga taaagtagaa gtgggggatt tcgccattgc catcggcaat ccgcacggtt
     2821 tgcagtcgac gctcacaacg ggcgttattt cggcaatcaa tcgggattta aatcacgacg
     2881 ggttacctaa attacaaacc gacgcttcaa ttaacccggg gaactcgggc ggcccgctca
     2941 tcaatatccg tggtgaagtc atcggtatca accaaatgat ttattcacaa acaggtggca
     3001 gtatcggctt aggctttgcc atcccggtaa accacgcaaa acaagtgatt gaaaaactca
     3061 aagcgggtaa aaaaattcgt tacgggtatg tcggcttaca ggtcgacggc gaacccaccg
     3121 atgagaagct cgatgcgctg ggagtgaaag gtaaaacagg ccttatcatt aaccaagtga
     3181 cgctcaactc gcctgcgttc aaagcgggtc ttgccgcgta cgattttatc acccacgtcg
     3241 acgggaaacc agcagaaaat ttcggagtgc tgaaatctgc agtgactcaa aaaggccctg
     3301 gcgaaacgat tgaacttaaa attatccgcc aaggtaaaga aataacggtg aaagtaaaaa
     3361 ttgctgaagc accagtacaa gacgcgcggt agtaacgagc ttcgatgacg cttaccatcg
     3421 acgtcggtaa ctcgcatacc gtcgtgggtt tttggcgaga gcatgcgctt gtgcatactc
     3481 ttcgtttggc gagcgatgca aggcgcaccg ccgatgaatg gcggctcatg cttctgcaag
     3541 cgctgagaga aaattctttt tcacaaattt caaaagcttc gctgtgctcg gtggtgcccg
     3601 cagcgcggct tgcgctgaac gaggcggtga ggcagttggg tgttacagaa ccgttgtggc
     3661 tctcgtcgac aacaccgctc aattttactt ttgcataccc acagccgcaa atgttggggg
     3721 cagaccgcct cgctgacatg attgccgcac gccattattt tggaacaaac tgcattacta
     3781 ttgattttgg tacggcggtt actttttccg tgcttaccga tggcgaattt gtgggtggtg
     3841 tcattgcacc tggtatcacc tcgtcactgg aagcgctttt tagcgcgacc gccaagttgc
     3901 cacaaattgc attccgccgc acgcaaaacg ccgtcggtca atcgactgtc gaatcgattg
     3961 aaattggagc atacttcggt tggcgtggta tggtgcgcga aattctcact gaaataaaaa
     4021 gagaattgcc cgagcacggg aaaaaccatt gcctcgttgc cacaggcggg attgttgaaa
     4081 atattgaatt cgcacccgat ttttttgatg tagtcgataa aaacctcact ctcaaaggac
     4141 tttatcttgc gcttcaaaat gcaaaggcgg tacacgattg aggttgcccc gtttttatat
     4201 tctcgaccgc tatctcttaa aacaattttt accgtcactt tcggtggcgt gcgtgttttt
     4261 tacttttatt attttaattt tttttcttaa agaaacaatc agacacgccg ttgaaaaaaa
     4321 tttagatgtt tgggtaattc tcgaactctg ctggtatgcg ttggggtgga cgctcactct
     4381 caccataccg atggcatttt tactttcaac tatactcgtt gtgggtgcgc tcaacgccga
     4441 ttctgaaatt atcgcgttac gggcgggcgg catcacatat ccgcgcatct tacggccttt
     4501 tctttttgtg gccatggctg cgtcgatttt cttagtttgg ttttcaaatt ttgtcgtgcc
     4561 tagatttgcc gacaatatgg aaaacgtccg caagtttatt ttgaccactg accccattgc
     4621 tgcaattcaa cccgggcaat ttgtaaatct cgaccgcaat gaaagttttg tgcgcaaaat
     4681 ttatatcgaa cgggcggtga aagatgaagt gggtaacggc gaaacgttca aaaatattca
     4741 ggtgagaaaa attgccagca tcggcggtat ccgtcggctt tctgaactca ttatcgcgca
     4801 ggaaggacgc aagatactta aaaaaactat ccaaggaaat tggataaagg cgttgcgcct
     4861 cacaaagggc tatctttttg ctaccagcag cgacggcaca tttcaacgca tcgatttttc
     4921 aaacgggatg ctggatctga atatacagga aaggagtcaa gttagacaaa tgaaacgcat
     4981 cgacgatatg cgcacgctca cactgcccga gcttatcaat agttacgaga ttacgaaaga
     5041 ctacccgaat gcgaaaaaac tgatactaaa agccgatttg gaattgcaca aacgatttgc
     5101 gctttcactt tcgacgatac ttttcgtgct tatgggattt cccttggcgg tggtcaatcg
     5161 gcgtagtggt aaagggatgg ggttgggaat ctctgtcgtt tttattttcg gttattttgc
     5221 gatttttcta tcggcggaaa tggtgtcggt tgatcgcacg ttgctttcac ctttcgttgc
     5281 ggcgcatgca gcgaacgcat tgctattttt gggcgcgctt gtttttatat tgcgtcgact
     5341 taaagaaatt tcactgcgcc ggctctttgg atttggccgc gttaaataaa agtttttatc
     5401 ccaagtgcat tgcaatcatg cgtgcaaaga tgtcatacac ttgaggattt tctttggtaa
     5461 attcgatacc atacttaaag cagtcatcgt ttccatcttt gcgccgccaa acgattacgg
     5521 cgcgtaagtc gagaatttta tcgggattat cttcggcgac aacccaaacg gtcatcgctt
     5581 gattttcacg atactctgtg tacgattgaa acgacaaccc actttgtgaa acgtcgataa
     5641 gcgctgggga ctcaaaaagc tccggtgttt ttaattcggc gttgtacgcg gaaatccata
     5701 cttggttgat atttttttta cgagggtttt tccgtcgatc gcgctgcggt tgttcaaaaa
     5761 aatcaagaat ttttgcacgc aacacttgcc gttgtaccgg ttgctgcaat agatacgcca
     5821 cttcgttcaa gatagttgag ttgagcaccc ccggtgcttc agggtcgtaa atcataaaaa
     5881 tgggtacgtc tgcgtatacc tcacggattg ccgataaatc agcgcgaaac gaatcaccca
     5941 actcaatcgg tgtgatgaca atcaattcga cagatgccgc ttcgctggat ttcggcaatg
     6001 tattgtaaac ggtggcaccc agcgtcgcga gtaatttagc ggtaagagtg tctccccccc
     6061 acgatagcga gagaaccttt ttaccggtta atggtttgaa gcgaaactgt tcgagtgctt
     6121 tgtcgagact tagcctagaa aaaggttttt caagcaggtg atcggtgatt gagagaatcg
     6181 gttgagaaat tttaccgcgg tctataaatt cgtgtaagag taaaatccga aacgaggcat
     6241 attttttttt gagctgaccc aatttttcag caaggttttt cacttcgtta tagtgcgcaa
     6301 taagaatatt gtcggtctcg tcgcttgttt tgatttcgtc aaaatggtgg atgaagtgta
     6361 cgctggcttt gcgtgcggtc aaataccctt ctaacgcatc ggcgacgatt ttactttcac
     6421 caaaaataaa aaattgggag tcggcgagcg acgaagttgc cacgaaacct atttttgaaa
     6481 cggtgcaacc gcgttaattc atttttaacg cactacgcac cgacgaaagc agacgctctt
     6541 cggtaaaggg cttgatgatg aagtcgtgcg caccggcggc gaccgcttct ttgataaacc
     6601 ggctttcgcc caacgccgaa accacaatca cttttgcgtc ggggatggct tgtttcacaa
     6661 gtttagtcgc atcgataccg cctaaatttt tcatggagag gtccatcgtg atgaggtccg
     6721 gttttaactc ttctgccatg ctcacgactt gtgtaccgtc ggtggcctcg cccacgattt
     6781 caaagccttg cgattccaga tggcggcgga taacttggcg catataaccc gaatcgtcga
     6841 caatcaagat tttctttttt tcagacatta ctgactcaat aactttattt ttaatttatt
     6901 ttggaggatt tactttacga tttctcatag ctatccacat ttctggaaaa taagcgccga
     6961 taatgatgag cgctaatcca aagaatgtcc atgattggaa gatggggcgt tcgctcactc
     7021 caccacccgg cactgagaac atttgaaatc gattgatagc aacgacgttc atgctaaatg
     7081 cccagcccgc taaaatgagc actaagccaa taacatgcgc tacaaaatag gtcttaattt
     7141 tttgccacat aaatgcagct tcttttcgtt tcatgccgct gcaagcgtaa aacaggtacg
     7201 cttgtccgtt ttctggaagc ctgtgcgtca actcgcttac gtcgacgctg tggttgcccg
     7261 attaccctct ttcgctgacg ggacgcacaa taaaacgaat caagtaatcg ttcttttctc
     7321 gacgcactgt ttcacgctca ctaaacgcaa tcatggcaac gattaacgag caagatatcc
     7381 gtgcaaaaat tgcaaaacgg attgtgagtg ctggggcacg ttttgcattt aacgagcgca
     7441 ctcctttgcc gacgctgcgt tggatcatcg atcaaattcg attcatcgaa cgccttgcgc
     7501 ccgacgttga actcaccgaa ggcgttgtcg atttaatcga gtatgatttt tttacccctg
     7561 ccacattcca acctgggcgc attttactct acagccacgg cggagcgtac accatgtttt
     7621 cccatcgcac acaccggagc ctggtgtcgc gtttagctct tgagttcaac gcacaagcta
     7681 tcgcacacaa ctaccgcctc gcccccgaac acccctttcc tgctgcggtg gacgacacaa
     7741 tacgcattta tcaatacctt ttatcgttgg ggtatgcgcc cgcaaacatc attgccgctg
     7801 gcgattctgc gggtggtggt ctcacgtttg cgtttatgct cgctgcgcgc gacaaaggat
     7861 tgcctttgcc acggttcgct atcggcatct caccgtgggt ggatctcacc gctgtgggcg
     7921 acagcatgca agagaacaag gctaccgacg tgatgctctc ccccgaagga attaaaaaat
     7981 tcgggctgcg ttatgtccaa gatgtaaata aactcaaaca cccgtatgca tccccgcttt
     8041 ttgccgactt acgcggattg ccttctgttt ttctgcaagc cgctggtgat gaaattttgc
     8101 gcgacg
//
LOCUS       DAOMPD010000050         8070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3851_length_8070_cov_2.586276, whole genome shotgun sequence.
ACCESSION   DAOMPD010000050 DAOMPD010000000
VERSION     DAOMPD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3851_length_8070_cov_2.586276"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..791)
                     /locus_tag="PLY93_02840"
     CDS             complement(48..791)
                     /locus_tag="PLY93_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68443.1"
                     /translation="MKPTLKLPDDNKLLAFIFARFIDGELTGLKEGNTLNYAPTLDAA
                     DFLAKQIRDEIRHARMYRTLYQSVSDTSTLSSPWLLKLIMNPIHGRLWTEHCFLDKAV
                     GERWVLYLMETIAAHIEDRKIKNYLNTIAKDERTHIAFGEEQTRLKAAQSAFWRFYLW
                     GLFLRVDYAMGLAYKLTRTLILKHYSAAAAEILTTFFTDARQKIRQESAVLLAVRGNV
                     SLWRMCFAQIIFWLRWPFCGWLQNPKKNF"
     gene            complement(805..1488)
                     /locus_tag="PLY93_02845"
     CDS             complement(805..1488)
                     /locus_tag="PLY93_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68444.1"
                     /translation="MVQFILNPTYGAVLIALNALFFFYALFKKNSLRTYLLFFVATTT
                     VELLFVSKILPVESAEVEATVAYIFTLTSDLRFILILAFLLYAQKNISDLGSLHITGD
                     VLKPAFIFTLFPTLIVTALGFLKPHWINQGGNRYLAYEIAFTVLTLLWVFVVLPQKTI
                     GTAEKFLMKKAALPVLAYYGLAVMADLGVMHDPHKQQLIKILSALTRYGIFLPWLVVQ
                     QKVIEKRSA"
     gene            complement(1494..2477)
                     /locus_tag="PLY93_02850"
     CDS             complement(1494..2477)
                     /locus_tag="PLY93_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP68445.1"
                     /translation="MQTLPRRRLTFFFLALFIFVGVFAPFIASSQPILIYDQTQYHKV
                     TGQTLKTSPLLYTFFYSDAQIEKVEYRKLALEGKIGAVFTLIPYSPYETELENYLKAP
                     SFTGRFKHFLGTDENGRDVAARLVRGTRNSLLVSLVAVGLSLLIGIAVGAIAGYFGGW
                     VDNLISRFIEVVICFPKLILIIAVMALKKPSLLNLMIVIGITSWTGVARLVRGEVMRI
                     RHYDYIDSARAMGAPSWRIIFLHILPATVGPLSVTAAFDVAEAVLTESALSFLGIGVP
                     IPEPSWGDVLKTAQDFPDIAWWMTLFPGILIFMTVITFNNLGDWLRSRFAK"
     gene            complement(2481..3482)
                     /locus_tag="PLY93_02855"
     CDS             complement(2481..3482)
                     /locus_tag="PLY93_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP68446.1"
                     /translation="MGYILKRFLLMVPTFIGIVTITFFLTKLRPDALTTASMGPDGMK
                     ESTGLDEFQKKMRAYYGLDKPLHEQYLQLWKNILTLDFSESRIDHRPVLAKIGEAVPI
                     TLFFNLITVFIVYTISIPLGIYMAIHDNSRREKFLATFLYVLYALPSFWVALMLLKYF
                     GSAEHLDLFPLSGLVSSYYDKLNWYQKIGDVLWHIALPIAVMVYGSFAFLSRYMKSSF
                     LEALKADYVRTARAKGLKNKTVIYIHALRNSVIPLVTLLGGLLPSLIGGSVILERIFS
                     IPGMGKLAYDSFYANDDTVIIAIVSISSILTMVGIFLSDIAYMLVDPRIRYGMTEDH"
     gene            complement(3719..4363)
                     /locus_tag="PLY93_02860"
     CDS             complement(3719..4363)
                     /locus_tag="PLY93_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP68447.1"
                     /translation="MTTASRYRHFIFTMFETLKDEEIIRHLKRAELSDTLLGKAESND
                     ATRLDAERIFAILVERHHASFTRLVTLRFGLDFSSADEITQDFWLECYTALPRYNPDR
                     PFLSWATTILFRTAERFLHKRRKEIQISPQSDFFAQQISPHESLEENAINDAQIKEML
                     AALRHLPQDLALLIELRFFQNKKIEDICEQTGLARSTVFEKLNLAYKKLRAHIQ"
     gene            4630..5172
                     /gene="pth"
                     /locus_tag="PLY93_02865"
     CDS             4630..5172
                     /gene="pth"
                     /locus_tag="PLY93_02865"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRP68448.1"
                     /translation="MAEKILVVALMNPGEKYRFTRHNAGAMALDLLFPDADFSLTKSL
                     EAEIAEARTPNENFYLLKPQTFMNLSGRSVQKALTKFSIDLKKTIVLHDEVELAVGEV
                     RYKFGGGHKGHNGLRSIMDILGKGDFHRIRIGVGRPQNDTRGIADYLLSFQSEKERAK
                     KEALLPVWETALKAIHPNNA"
     gene            5228..5659
                     /locus_tag="PLY93_02870"
     CDS             5228..5659
                     /locus_tag="PLY93_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRP68449.1"
                     /translation="MKKTLLTLLAFGLLTSGVMADDISGVWKTVDDETGQAKSYVNIW
                     VHNGTAYGKITKLLNRKPNEDPDPVCTECKGQNNGKKIVGLTIIWGLKEDGGEWTGGH
                     IMDPKNGKTYKCKIKREGNTLKVRGFIGFSLLGRTQTWHKL"
     gene            5742..7820
                     /gene="fliD"
                     /locus_tag="PLY93_02875"
     CDS             5742..7820
                     /gene="fliD"
                     /locus_tag="PLY93_02875"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRP68450.1"
                     /translation="MPAPQMSGLASGIDTKDIVRKLVEVERAPIVRLENNKRELSDTS
                     KALTELRKRTKTLQDSLHAMTSFEAAFEQKKLLTEPQGFLDGTVRKNATPSQHLVHVL
                     KLASKLSIATSPVESNKKLSAAKIKIGDTTSNFTGGTLQSLREHLQKYHSKQLTSSVV
                     RVKENESILVIDSVEEGEDALLKIEDPDGLLKSLGLLSGATLPDTGKKNDKPTQNPDS
                     KDAPQKNEKETEESERWSVNPTQIELVSGRAAQIADDKKSFALPDDSVSRYRFLASQL
                     TSPQKRLTKISLSAISATEQENNDNAPDSLSDGVRDNLNIKGIQLETYNITRTRQKEA
                     ARESDYGVVLHLASGDEKKISLKGKLGKQELLVDAALSSVDFYSSGIDVLVEPIELTY
                     AVKPQPKVSPADEKKSLTPLEEEQRKVFPNLIQAAQNAELKIDGILVTRKTNKNIGDI
                     IDGVSLNLLKPSPDNVKTEILNDTESAQKQVLNFVKAYNDLLQFAEENAKAARVKEAG
                     KFQEMRKESGILATNATIRQLINGLKMHTSNAYPALRDPHFRTLSQIGISTGAVGADR
                     EEVIKGYLQVDEAKLTQAFAESPRAVKELFASDTNNDLRIDNGYAYVTDKFLDPFTRF
                     TRGLIAEQIKSNAERTKQIDRDIKRQEEHVKNYEAKLKTKFGYMESSVMKNKSTGKFL
                     KQKLGISDAQ"
     gene            7864..>8070
                     /locus_tag="PLY93_02880"
     CDS             7864..>8070
                     /locus_tag="PLY93_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP68451.1"
                     /translation="MPAQKKISVVIPALNEERDLPELLSSLKAQSFRNFEVIVADAGS
                     KDKTREIAQEFGARVVDGGMPGVGR"
BASE COUNT         2380 a         1751 c         1993 g         1946 t
ORIGIN      
        1 gctttccgga gcgaaggagt tttgttttat aggggcgttc atagtaatta aaaatttttc
       61 ttaggatttt gtaaccagcc gcagaaaggc caacgtagcc aaaaaataat ttgcgcaaaa
      121 cacatccgcc acagcgatac attaccgcgc accgcaagta gtaccgcgct ttcttggcgg
      181 attttttgtc gagcgtccgt aaaaaacgtg gttaaaattt ctgccgccgc tgcagagtaa
      241 tgtttgagga tgagtgtgcg cgtgagtttg taagctaacc ccattgcata atcgacacgc
      301 aagaaaagtc cccacaaata gaaacgccag aacgcacttt gcgcggcttt cagtcgagtc
      361 tgctcttcgc caaaggcaat gtgcgtgcgt tcgtcttttg cgatggtgtt tagataattt
      421 tttatttttc gatcttcaat gtgcgcggca atcgtttcca tcaagtaaag tacccagcgt
      481 tcgccaacgg ccttgtctaa aaaacaatgc tctgtccaga gccggccatg gatggggttc
      541 atgatgagtt ttaagagcca cggggaagaa agtgtggatg tgtcggaaac actttggtag
      601 agagtgcgat acatgcgggc gtgacggatt tcatcgcgga tttgtttggc gagaaaatcg
      661 gcagcgtcta aagtgggggc atagttaagc gtattgcctt ccttgagccc tgtgagttca
      721 ccgtcgataa agcgcgcaaa aataaaggcc aagagtttat tgtcgtcggg gagttttaaa
      781 gttggcttca ttatattttt tactttatgc gctacgcttt tcaatcactt tttgctgaac
      841 cacgagccac ggtaaaaaga ttccatagcg ggtgagcgcg gaaaggattt taatgagttg
      901 ttgtttgtgt ggatcgtgca taacacctag gtctgccatc acagcgagtc catagtacgc
      961 caaaaccggt agcgcggctt ttttcatgag aaatttctcg gcggtaccga ttgtcttttg
     1021 tggcaacacg acaaaaaccc aaaggagggt taagacggta aaagcgatct catacgccaa
     1081 gtaacgattg ccgccttggt ttatccagtg tggttttaga aaaccgagcg cagtgacgat
     1141 gagcgtggga aaaagagtga agataaacgc gggtttgaga acatcccctg tgatgtgcaa
     1201 agaacctaaa tcggaaatat ttttttgtgc atagagtaaa aatgcaagga tgagaataaa
     1261 gcgcaaatca ctggtgaggg tgaaaatata cgcgacggta gcttctactt ctgcactttc
     1321 aacaggtaaa atttttgaga caaaaagaag ttcaaccgtg gtggtggcga caaagaaaag
     1381 taagtatgtg cgcaacgagt tctttttgaa aagcgcgtag aaaaaaaaga gtgcgttgag
     1441 agcgatgagc acagcgccgt aagtggggtt gagaatgaat tgtaccatag aatttattta
     1501 gcaaagcgcg aacgcaacca atctcctaga ttgttaaaag taatgactgt cataaaaata
     1561 agaataccgg gaaagagcgt catccaccag gcaatgtcgg ggaaatcttg cgctgttttg
     1621 agtacatccc cccatgacgg ctctgggatc ggcacgccga ttcccaaaaa gctgagcgcc
     1681 gattcggtga gcaccgcttc ggctacgtcg aacgcggcgg ttaccgagag tggtcccaca
     1741 gtcgcgggta aaatgtgcag aaaaataatc ctccacgacg gtgcgcccat cgcgcgcgcg
     1801 ctgtctatat agtcgtagtg gcggatgcgc atcacctcgc cccgtacgag gcgcgcaacg
     1861 cctgtccaac tggtgatacc gatgacaatc attaaattga ggagcgatgg ttttttgaga
     1921 gccatcactg cgatgataag aatgagtttg gggaaacaga tcaccacttc gataaaacga
     1981 ctgatgagat tatccaccca accgccgaaa tagcctgcta tcgcgccgac ggcgatacca
     2041 atgagaagag aaagaccaac cgccacgagc gaaactaaaa gcgagttgcg cgtgccgcgc
     2101 accaggcggg ccgccacgtc gcgaccattc tcatcggtgc ccaaaaaatg tttgaagcgg
     2161 ccagtgaagc tgggggcttt caaataattt tcaagctctg tttcgtaagg tgagtagggg
     2221 atgagcgtaa agaccgcgcc gattttgcct tcgagcgcaa gcttgcgata ttccactttt
     2281 tctatttgtg catcgctata aaaaaaagta taaagtaacg gggatgtctt taaagtctgc
     2341 cctgttactt tgtggtattg agtttggtcg taaatcaaaa ttggttgcga cgacgcgatg
     2401 aaaggcgcaa agacgccgac gaaaataaac agagccaaga aaaaaaacgt taatcggcgg
     2461 cgcggcagag tttgcatgcg ttagtggtcc tcggtcatgc cgtatcgaat gcgggggtca
     2521 acgagcatgt aggcaatgtc agaaagaaaa atgcccacca tggtgagtat cgacgaaatc
     2581 gagacaatcg caatgataac cgtgtcgtcg ttcgcgtaaa aactatcgta cgccagttta
     2641 cccattccgg ggatggagaa aatccgttct aaaattacag agccgccaat gagcgacggg
     2701 agtaatccac ccaagagggt gactaatggg atgactgagt ttctgagcgc gtggatgtaa
     2761 attaccgtct tatttttaag tcctttggcg cgtgcggtgc ggacgtaatc cgctttgagc
     2821 gcttccaaaa aagatgattt catatagcgt gataaaaagg cgaacgaacc gtaaaccatc
     2881 accgcgatgg gcagcgcgat atgccacagc acgtcgccaa ttttttggta ccagttgagc
     2941 ttgtcgtaat acgaactcac caggcccgac agcggaaaaa ggtcgaggtg ctctgccgaa
     3001 ccaaagtatt ttagcagcat gagcgccacc caaaaactgg ggagcgcgta gaggacataa
     3061 agaaaggtgg ctaaaaattt ttcacggcga ctgttgtcgt ggattgccat ataaattccc
     3121 agtgggattg aaatcgtata cacaatgaag actgtgatta gattaaaaaa gagagtgatt
     3181 ggtaccgctt cgccaatttt agcgagtacc ggcctgtgat cgatgcgact ttcggaaaaa
     3241 tcaagagtta aaatattttt ccatagctgt aaatactgtt cgtggagcgg tttatccaac
     3301 ccataatacg cgcgcatttt tttctgaaac tcgtcgagtc ccgtgctttc tttcatgccg
     3361 tctggcccca tcgaagcggt ggtgagcgcg tcggggcgca atttagttaa aaagaaggta
     3421 atcgtcacga tgccgataaa cgtcggtacc atgagcaaaa aacgtttgag gatgtaaccc
     3481 acgcgcctac gcaaacagag ccgtgaaagg gtgggtcaag ccttaaacga ctatactttt
     3541 acgtattgct caaattcaag ttctaaatgc aacgatttgg cttttgttga attttctctg
     3601 taaaactgct ggtttgtcag ttcaaaagtg caaacgtcgc ggtttaagag agcaagcaca
     3661 attaacagga cgttatgtag aagaagtaaa ttgatccttc cataagaagg tagggtaact
     3721 attggatatg cgctctcagt tttttataag ctaaattgag tttttcaaaa acggtcgagc
     3781 gtgcaagacc ggtttgttcg cagatatctt caattttttt attttggaaa aaacgtagct
     3841 caatgaggag tgcgaggtct tgtgggaggt ggcgcaatgc cgcgagcatc tctttgattt
     3901 gtgcgtcgtt gatagcattt tcttcgagac tttcatgcgg acttatttgt tgagcgaaaa
     3961 aatcgctctg cggtgaaatt tgaatttctt tacgccgctt gtggagaaag cgctctgcag
     4021 tgcggaaaag aattgtggtc gcccacgaca aaaaagggcg gtcggggtta tagcggggaa
     4081 gggcggtata gcattcaagc caaaaatctt gggtgatttc atcggcgctc gaaaaatcca
     4141 gcccaaagcg cagcgtgacg agcctcgtga aagaggcgtg gtggcgttcg actaaaatag
     4201 caaatattct ttctgcgtcg aggcgtgtcg cgtcgttact ttcggctttg cctaaaagcg
     4261 tatcggaaag ttccgcgcgt tttagatggc ggataatttc ttcgtctttt agcgtttcaa
     4321 acatcgtaaa gataaaatgc cgataccgag acgccgttgt catttgcaga attgaacccg
     4381 caaataatta tggaagccac gaggcgcgcg tgactcttcg gggtgtaccc ctacttttac
     4441 acgcgcccaa gttgggttta ggacggatta tccgcgcttc ccttaagctt ccaggtttgt
     4501 aacattggtt caaatctaga aaaagaaagc ccgaacgtct cagaaaccgg cgagcctata
     4561 taataggtat atttcacctt gcaagtacaa aacggttggc gcacagattc gaatttggat
     4621 gtcgtattcg tggcagaaaa aattcttgtt gttgcattga tgaatccagg agaaaaatac
     4681 cgtttcactc ggcataacgc cggtgcaatg gcgctcgatt tacttttccc cgatgcggat
     4741 ttttcattaa ctaagtcgtt ggaggcagag attgccgaag cccgcacccc caacgagaat
     4801 ttttatcttt tgaaaccgca aacgtttatg aacctttcgg ggcggagcgt tcagaaggcg
     4861 cttacgaaat ttagcatcga tttgaaaaaa acaatcgtgc tccatgacga agtcgaactc
     4921 gccgtcggtg aagtccgcta caagtttggg ggaggtcata aagggcacaa tgggctgcgc
     4981 agtattatgg atattctggg caagggcgat ttccaccgga tacgcatcgg cgtcggccgt
     5041 ccacaaaacg atacgcgtgg tatcgccgat tatcttttat cgtttcagag tgaaaaagaa
     5101 cgtgccaaaa aagaggcgct tttacccgtg tgggaaacgg cgctaaaagc aatacacccc
     5161 aataatgctt gaccgtacta aagagtctgt gcaaaaaacc cacacgaatt attttggagg
     5221 aaaaacaatg aagaaaactt tactaacctt actcgccttt ggtctattga catcgggcgt
     5281 tatggccgac gatatttcgg gcgtatggaa aaccgtcgac gatgaaaccg gccaagcaaa
     5341 atcgtatgtg aatatttggg tacacaatgg caccgcttac ggcaaaatta caaagttatt
     5401 gaaccgtaag ccgaatgaag atccagatcc ggtatgcacc gaatgcaaag ggcaaaacaa
     5461 cggtaaaaaa attgttggct tgaccatcat ttggggactc aaagaagacg gtggcgaatg
     5521 gacgggtggc catattatgg acccaaaaaa cggtaaaacc tataagtgta aaatcaagcg
     5581 cgaaggcaac acgcttaaag tacgtgggtt tatcggtttc tctcttttag gccgcacaca
     5641 aacttggcat aaactctaag agtactcatt tttatggtca ataggaaaaa cgctttagcg
     5701 ttttccctat tgaccgatac tcaagatagg ggacataaga catgccagct ccacaaatgt
     5761 ccggtcttgc atcgggaatc gataccaaag atatcgtacg aaaactcgtc gaagtcgaac
     5821 gcgctccgat tgtgcgttta gaaaataata agcgcgaact ttccgacacg agtaaagccc
     5881 tcactgaatt acgtaaacgc acaaaaacat tacaagattc tctccacgcg atgacctctt
     5941 ttgaggcggc gtttgagcaa aaaaaacttt taaccgaacc ccaaggtttc ctcgacggta
     6001 cagtccgcaa aaatgccacg ccttcgcagc atttggtgca tgtgttgaag ttggcctcaa
     6061 aattaagcat tgcgacgagc cctgttgaat cgaataaaaa actgtctgcc gcaaaaatta
     6121 aaatcggcga tacgacctca aattttacag gcggcacgct acaatcgttg cgcgagcatt
     6181 tacaaaaata ccatagtaaa caattaacat cgagcgttgt gcgggtgaaa gaaaacgaat
     6241 cgattttagt catcgatagc gtggaagagg gcgaagatgc actccttaaa attgaagacc
     6301 ctgatggatt gctaaaatcg cttggtcttt taagcggcgc cacgctaccg gataccggaa
     6361 agaaaaacga taagcctacg caaaatccag attccaaaga cgcgccacaa aaaaatgaaa
     6421 aggaaactga agaaagcgag cgttggtctg taaatcccac tcaaattgaa cttgtctcag
     6481 gtcgtgccgc gcaaatcgcc gacgataaaa aatcgttcgc tctccctgat gattcggtga
     6541 gtcgctaccg ttttcttgca agtcaactca cctcgcctca aaagcggtta acaaaaattt
     6601 cactttctgc gatttccgct acggagcaag aaaataacga caatgcccca gatagtttaa
     6661 gcgacggtgt gcgtgacaat ttgaatatta aaggtattca gttggaaacg tataacataa
     6721 cacgcacgcg tcaaaaggaa gcggcgcgcg aaagtgacta tggcgttgtg ttgcatttag
     6781 catcggggga tgaaaagaaa atttcattaa aaggaaaatt gggtaagcaa gagttactcg
     6841 tggatgctgc gctctcgtcg gttgattttt actccagcgg tatcgatgtg ctcgttgaac
     6901 cgatagagct gacgtatgca gtaaagcctc aacctaaagt ttccccagcg gatgagaaaa
     6961 aatcactcac ccctctcgag gaagagcagc ggaaagtgtt cccaaattta atccaagcgg
     7021 cacaaaatgc agagcttaaa atcgacggta ttttagtaac tcgtaaaacc aataaaaata
     7081 ttggcgatat tatcgacggc gtgtcgctca atcttctgaa gccttcgccc gacaatgtca
     7141 aaacagaaat tttgaacgac accgaaagcg ctcaaaaaca agtcttgaat tttgtgaaag
     7201 cgtataacga tctactacag ttcgccgagg agaacgccaa agcggcgcgc gtgaaagaag
     7261 ccggtaagtt tcaagaaatg cggaaagaat ctggtatttt agccaccaac gcgacaatac
     7321 gccagctcat caacggtctt aaaatgcaca cctcgaatgc atatcccgcg ttgcgcgacc
     7381 ctcactttcg cacactatcg caaattggta tctcaacggg agctgtcggt gccgaccgtg
     7441 aagaagttat caagggctat ttgcaagtcg acgaagcaaa gctaacgcag gcgtttgccg
     7501 aatcgccgcg tgcagtcaaa gaactttttg caagcgacac caataacgac ttacggattg
     7561 ataacggcta cgcgtatgtc accgataagt ttttagaccc ttttacgcgc tttacccgag
     7621 gcctcattgc cgagcaaatt aaatcgaacg ccgaacgaac aaagcaaatt gaccgcgaca
     7681 tcaaacgcca agaagaacac gtaaaaaatt acgaagcaaa gttaaaaaca aaattcggtt
     7741 atatggagag ctcggtgatg aaaaacaaat caacgggtaa attcttgaag caaaaattgg
     7801 gcataagcga tgcgcaataa aattatttct gtacagaaag tgtaaccttc accgcagtgc
     7861 tgtatgccag cacagaaaaa aattagcgtc gttatccccg cgttaaacga agagcgcgac
     7921 ttacccgaac ttttatcttc tctcaaagcg cagtcgtttc gcaattttga agtgattgtc
     7981 gccgatgcgg gctctaaaga taaaacgcgc gaaatcgcac aagagtttgg cgcgcgcgtc
     8041 gtcgacggcg gcatgccagg ggtggggcgc
//
LOCUS       DAOMPD010000051         7979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3900_length_7979_cov_2.724003, whole genome shotgun sequence.
ACCESSION   DAOMPD010000051 DAOMPD010000000
VERSION     DAOMPD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3900_length_7979_cov_2.724003"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..519
                     /locus_tag="PLY93_02885"
     CDS             13..519
                     /locus_tag="PLY93_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRP68452.1"
                     /translation="MVGALDDFISWGQSYSLWPFPFATACCGIEYMATAGADFDIARF
                     GAERPSFSPRQADMILVLGTITYKMAPVLRNIYEQLADPRYVVSVGACASTGGMFHTY
                     AVLQGIDRIMPVDVYVPGCPPRPEAILEAVMQLQKKLRSQTIEERREEVRKKLALIEK
                     QNRPVVVE"
     gene            516..881
                     /locus_tag="PLY93_02890"
     CDS             516..881
                     /locus_tag="PLY93_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRP68453.1"
                     /translation="MTSTQYYDIQDSRRIFGSEGELMRFLKTLLIQDYKIFEMQNEQG
                     TLVPYHAHAFNETILVLEGMVRFIVEEDIVDVYPGQIITIRPFAIHLAAFPKEEGARF
                     YMLFSERGRQNIFNEFQRK"
     gene            complement(869..1816)
                     /locus_tag="PLY93_02895"
     CDS             complement(869..1816)
                     /locus_tag="PLY93_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68454.1"
                     /translation="MLSLFVLFCSCYLSLLTTHLSAQTPEAVKEEIIYYLDNALYHYE
                     RRRFLNTLDNLRLVLDLLEKTKDERVLKLTAETLAAETLKNLGREEEAVNMYERALAH
                     GLNNNAANAYLALYFDRHKDFKRALGHFEKYFENDKKDVSTHIRYAALLGRLKKREQA
                     KKVLAEIDATPSPQKVSDCKTLEIKKKWRNAVTCFEVIRQALPASEEGYLGLYRAARV
                     LRDKALFQQNAELLYFIFGNETRYIWPLVEYNISQKEFYEARLLLEEIILLRGKDSEA
                     ERILANLKIDAEKAIKKPFRASDKELEFYEEMRKNNYLR"
     gene            complement(1833..3386)
                     /locus_tag="PLY93_02900"
     CDS             complement(1833..3386)
                     /locus_tag="PLY93_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68455.1"
                     /translation="MQRKYHFNFLSLFCAWGVCVFSLSAQAKKESSRDIAAEVDKLNW
                     EGIDLSNDARYPAAAEKFQKAITLDDTRSARSYHNIGYTYELAGDKNKALDSYQKAVT
                     RNPQQTITWQSLGKLQYQMGLYKDAVMSGENVLKLDPRNVPVQKWLPDAYAKLAEQKI
                     YDARNDLSGTASDNPNCEARPDVIAEVGTYFTAVGVIDKRATAFKYYIPHGALQSLAG
                     LYAEFHPLRELTFRLNGHTPYFGVLQPNFFAGQENIEVQYNFKNFFFGAGILFTQLNV
                     ADSIIEGETATYIQNSDYTSLSDTKFGLFLGSQDELTYFALKVYPRYLFRGPTSGPKS
                     NSLDTVKIDLEYRHNLRLSLGSTIDENQPISKGPFTDLIFKMSIDEVYITEFKPVAGT
                     DALGHFFGVYDISLGMEFGKLQRQFDKVGFTYGFLLTERLYFQDVKNTDTTGFGNGQG
                     YFGFNTSGMTSGNAFSSFRNSSFIVTLFAAQMFRNRYIVREKIFFEATPGNERNHAVG
                     VQLSFGVRF"
     gene            complement(3462..3824)
                     /locus_tag="PLY93_02905"
     CDS             complement(3462..3824)
                     /locus_tag="PLY93_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68456.1"
                     /translation="MQITFFTYMEDALKKALSTGVQTLISVTEEVRHGLTVLDKDIQN
                     FRRQISLSNATDDTTAKIEALIQKSIATIRDYEERAVEISRTIQKRLAEVDPMAVDNI
                     QELTKKISLLNKEIIKTK"
     gene            complement(3833..4192)
                     /locus_tag="PLY93_02910"
     CDS             complement(3833..4192)
                     /locus_tag="PLY93_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP68457.1"
                     /translation="MLDKVKGLSYTIDDESKWSVVRLNGDLNMFSAPDLRSALVKKFE
                     SGTKRFIFNLSELSFVDSSGIGILVSFVSLAKKSEGSKIILCGLNPQIRNIFEVTRLM
                     SVFTVLDTLDDARDAMG"
     gene            complement(4592..5674)
                     /locus_tag="PLY93_02915"
     CDS             complement(4592..5674)
                     /locus_tag="PLY93_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68458.1"
                     /translation="MNFRIRSITPESLILQQTKGFVLVRVIFKIFALILLGAGALFVL
                     GEPQNTVAYMLLGFGIFLWGASDTAKNFSRQLPAKLIFDNQAGLLRFIQRVRKKNIET
                     VAVSYAEIADFKTITRRQDRVVRYFTALEWQDDSLLLLCSHNTQKKANEEVLKFRASI
                     ALHKKSEPVVFTPPVRVQEKSLADKTVFTWRFDFFNPKNRTTYLAVVSFSGVLVALGF
                     QLDILGKILAFAASGVILGIPFCFALRWVGAEGVFEIDKHEIRYWEKRGPKTELKHSI
                     LLRELERVWCVALGRSNDMAVYLLKKEEYELYLKIIEGEIAMGDIISALQILYRSFKI
                     YTPQLNFSERFRFAWCIRKIVTELRK"
     gene            complement(5822..6442)
                     /locus_tag="PLY93_02920"
     CDS             complement(5822..6442)
                     /locus_tag="PLY93_02920"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRP68459.1"
                     /translation="MFKLPELPYGKTGLAPFLNEEQMTFHYEKHHKAYIDNLNKFIES
                     DAALKGKTLEQIVLSSSGGIFNNAAQAYNHTFYWLNMAAQGKGGAPSAELGEAIKRDF
                     GSLDELKAKFVDGGVKTFGSGWIWLCADAAGKLSLVSTSNAAVPFTNNGPAPLLVADV
                     WEHAYYVDYRNARAKYLETFWSHVNWAFVSENYASKKVRDLTKTMS"
     gene            complement(6526..7869)
                     /gene="gdhA"
                     /locus_tag="PLY93_02925"
     CDS             complement(6526..7869)
                     /gene="gdhA"
                     /locus_tag="PLY93_02925"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRP68460.1"
                     /translation="MKLPGREEFLQRVEKRDPHQPEFLQAVREVTASLWPFLERHPHY
                     AQLGLLERLVEPERAIQFRVAWSDDKGEIQVNRAFRVQHSSAIGPFKGGMRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGAKGGSDFNPKGKSEGEIMRFCQALMLELYRHLGSD
                     TDVPAGDIGVGSREVGYMAGMMKKLTNEASCVFTGKGLSYGGSLVRPEATGFGTVYFV
                     EEMLQEARRETHGARVLISGSGNVAQYAALKARELGGKVITFSDSDGTLYAQDGFDDE
                     AIQSVMDLKNNRRGRLAELGEDARFEYISGKRPWHIPGEIALPCATQNELDENDARAL
                     VANGVVCVAEGANMPSTLEAVEVFENSKTLFAPGKASNAGGVATSGLEMSQNAMRLSW
                     SQEEVDKRLHGIMKSIHKNCVLHGKRRDGNISYVDGANISGFVKVADAMLGQGVF"
BASE COUNT         2162 a         1944 c         1681 g         2192 t
ORIGIN      
        1 gaccaaagct ttatcgtcgg cgcgctcgac gattttatta gctgggggca atcgtactct
       61 ttgtggccat tcccttttgc aacggcgtgt tgcggtatcg agtacatggc gacagcgggc
      121 gcagacttcg atattgcgcg ttttggcgca gaacgtcctt ctttctcgcc ccgccaagcc
      181 gacatgattc tcgtcttggg gacaatcacg tacaaaatgg cgccagtctt gcgcaatatt
      241 tacgaacaac tcgccgaccc acgatacgtt gtgtcggtgg gcgcgtgcgc atccactgga
      301 ggtatgttcc acacctatgc ggtgctccaa ggaatcgacc gtatcatgcc ggtcgacgtt
      361 tacgtacccg gttgcccacc ccgccccgag gcaattttag aggcggtgat gcaactccaa
      421 aaaaaattac gctcgcaaac gatcgaagag cgccgcgaag aagtgcgtaa gaaactagcg
      481 cttattgaaa aacaaaatag gccggttgta gtagagtgac ctcgacgcag tattacgaca
      541 ttcaagattc acgccgtatt ttcggcagcg aaggagaact catgcgtttc ttgaaaacgc
      601 ttttaatcca agactataaa atttttgaga tgcaaaacga acagggcacg ctcgtccctt
      661 accacgcgca cgctttcaat gaaaccattt tagtgctcga aggcatggtg cgttttatcg
      721 ttgaagaaga catcgtcgac gtttatcccg ggcagatcat tactatccgc ccgtttgcca
      781 tacacttagc ggcattcccc aaggaagaag gcgcacggtt ctatatgctt ttttcagaac
      841 gcggacgcca aaatattttc aacgaatttc agcgtaaata gttatttttg cgcatctctt
      901 cataaaattc caattcttta tcgctcgcac ggaatggctt tttaatcgcc ttttcggcgt
      961 cgatctttag atttgcaaga attcgctcgg cttcagaatc tttgccgcgc agcaaaataa
     1021 tttcttcgag caaaagtcgc gcttcgtaaa attccttttg actaatatta tattcaacca
     1081 acggccaaat atagcgcgtc tcgttgccaa aaatgaaata gagcaattcg gcgttttgtt
     1141 gaaacagcgc tttatccctc agcacgcgcg cggcacgata aagccccaaa tacccctcct
     1201 cgctcgcggg taacgcttgg cggatgacct cgaaacaagt gactgcattc cgccattttt
     1261 tctttatctc caaagtcttg caatccgaca ctttttgagg gcttggcgtc gcatcaattt
     1321 cggctaacac tttttttgct tgctctcgtt tttttagtct gcctaaaagt gctgcgtagc
     1381 gtatatgcgt ggaaacatct tttttatcgt tttcaaaata tttttcaaag tgcccaagcg
     1441 cacgcttgaa atctttatgc ctatcaaaat aaagcgcaag gtaagcattc gcggcattat
     1501 tatttaaccc gtgtgctaaa gcgcgttcgt acatattcac cgcttcttct tcgcggccta
     1561 aatttttgag tgtttcggcg gccagagttt cggcggtcaa tttcaacacg cgctcgtctt
     1621 tggtcttttc taacaaatcg agcacaagac ggaggttatc taacgtattc aaaaaacgcc
     1681 gccgctcata atggtagagc gcgttgtcta aataataaat aatttcttct tttactgcct
     1741 caggcgtttg tgcgctgagg tgggttgtaa gtagacttaa ataacaactg caaaaaagta
     1801 cgaagagaga gagcaatccc tctcttcgaa ttttagaaac gcacgccaaa agaaagctgt
     1861 acccccaccg cgtggttacg ctcgttgccg ggagtcgcct caaagaaaat tttctcgcgc
     1921 acaatatagc ggttgcggaa catttgcgcg gcaaataatg tcacaataaa cgacgagttg
     1981 cggaaggagg aaaatgcatt gcccgatgtc atgcccgatg tattaaaacc aaaatagcct
     2041 tggccgttgc caaagccggt ggtgtcggtg ttttttacgt cttgaaaata aagccgctcg
     2101 gtaagtaaga agccgtacgt gaaaccaact ttatcgaatt gtcgttgcag cttgccaaat
     2161 tccatcccta gtgaaatatc ataaacacca aaaaaatgcc caagtgcatc agtgccggcg
     2221 acaggtttga actcggtgat atacacttca tcgatactca ttttgaaaat taaatcggta
     2281 aacggccctt tgctaatcgg ttgattttca tcgatggtgc tgcccaaaga aaggcgtaag
     2341 ttatggcgat actctaagtc aattttaacc gtatcgagac tgttgctctt gggaccgctt
     2401 gtggggccac ggaataaata gcgtggatac acctttaaag caaaatatgt cagctcgtct
     2461 tgagagccaa gaaacaatcc aaactttgtg tcgctcaacg atgtgtaatc ggagttttgt
     2521 atgtacgttg ccgtttcacc ttctatgata ctatcggcga cgttgagctg tgtgaaaagg
     2581 atacctgctc caaaaaagaa attcttgaaa ttgtattgta cttcgatatt ttcttgacct
     2641 gcaaaaaaat tcggttgcaa cactccaaaa taaggagtat gcccattcag gcggaaagtc
     2701 aactcgcgca aaggatgaaa ttccgcataa agacctgcga gcgattgtaa tgcaccgtgc
     2761 ggtatataat acttaaacgc ggtcgcgcgc ttatcgataa cgccaacggc ggtgaagtaa
     2821 gtacccacct cggcaatgac atcgggccgc gcttcgcagt tgggattgtc gctcgctgtg
     2881 ccgcttaaat cattacgcgc gtcgtatatt ttttgctcgg caagttttgc atacgcatcg
     2941 ggcaaccact tctgcacagg cacattgcgc gggtcgagtt tcaatacatt ctcgcccgac
     3001 atcactgcgt ccttatataa ccccatttgg tattgcagtt tccccaagct ctgccatgta
     3061 attgtctgtt gcggattgcg tgtaaccgcc ttttgatagc tatcgagcgc tttatttttg
     3121 tcgccggcta attcatacgt gtaaccgatg ttgtggtatg accgcgcaga acgggtgtcg
     3181 tcgagagtaa ttgctttttg gaacttttcc gcagcggcgg gataccgtgc atcgttcgat
     3241 aaatcaatac cctcccagtt gagtttatcc acctcggcgg caatatcacg gctgctttct
     3301 ttctttgctt gggctgaaag cgaaaaaacg cacacccccc acgcacaaaa taaagataag
     3361 aaattaaaat gatactttcg ttgcatattt tattagacgt ccttaataga taaaatatcg
     3421 acgttctatg ctctcttttt gagtcttttg ttttagggac tctattttgt ttttattatt
     3481 tctttattca gcaacgatat tttctttgtc aactcttgaa tgttatcgac cgccatcgga
     3541 tcgacttcgg caaggcgctt ttgaatcgtc cggctaattt ctaccgcgcg ctcttcgtaa
     3601 tcgcggatcg ttgcaattga tttttggatt aacgcttcaa ttttcgcagt ggtgtcgtcg
     3661 gtcgcattcg agagagaaat ttgccgacgg aaattttgaa tgtctttgtc taaaacggtt
     3721 aatccgtgcc gcacctcttc agttacggag atgagagttt ggacaccggt gctaagagct
     3781 ttttttaacg cgtcttccat ataagtaaaa aaagtaatct gcataaggcg agttagccca
     3841 tggcatctcg cgcgtcatct aaagtatcta acactgtaaa gaccgacatg agccgagtga
     3901 cctcaaaaat attacggatt tgcggattga gcccgcacaa aataatttta gagccctctg
     3961 attttttagc aagcgaaaca aaactaacta aaatgccaat acccgacgaa tcgacaaagg
     4021 aaagctcgct caaattaaaa ataaaccgtt tcgtgccgct ttcaaatttt ttaactaatg
     4081 cagagcgtaa gtcgggtgcg gaaaacatat tgaggtcgcc gttcaagcgc acaacgctcc
     4141 acttagattc atcgtcgata gtgtacgata agcctttaac tttatctagc attcttatgt
     4201 aagcgtttca aatcgattca acaagtcaag tgatacaaaa agccgaattg ctctaatttg
     4261 aaagatagac gctaataact atgattagat ggatttaaaa attaatagcc tgtgcgtata
     4321 gaatcggatt ttctctcaaa ttttacccta attttaatca ctccagcact tacggtgcat
     4381 agttacccct taatgtgaca taatccaaga aataggatag ttatccctta atgtgacata
     4441 atatgaaaaa ttgagatagt tatccctacc gcttacatgc tccaacttgc caaaatacta
     4501 tgtcagtatt acactcggtt gattgaatac ttacgaatta tccgcggttg tttagagatg
     4561 tccaaaatta aggactgttg aacattcctt attatttcct caattccgta actattttgc
     4621 ggatacacca cgcaaagcga aaccgctcag aaaaattgag ctgtggagta taaatcttga
     4681 atgaccgata caaaatctgc agcgctgaaa taatatcacc cattgcaatc tccccttcaa
     4741 taatctttaa atatagctcg tactcctctt tctttagaag atacaccgcc atatcgttgc
     4801 tgcgtcccaa agctacgcac cacaccctct ccagttcacg gagcaaaatc gagtgtttaa
     4861 gctccgtctt tggaccccgt ttttcccaat accgtatttc gtgtttatcg atttcaaaaa
     4921 caccttctgc gccaacccag cgcaatgcaa aacaaaaagg gatgccaagg ataaccccac
     4981 ttgcggcaaa tgcaagaatt ttgcccagta tatccaactg aaaacccaag gcaaccaaga
     5041 caccggaaaa actaacgacg gctaagtaag tggtccggtt tttaggatta aaaaaatcaa
     5101 agcgccaagt aaaaacggtt ttatccgcaa gagacttttc ttgcactcgt acagggggcg
     5161 taaacacaac gggttctgat tttttatgta gagcgatact ggctctaaac tttagaactt
     5221 cttcgtttgc cttcttctgc gtgttatgcg aacataaaag caagagagaa tcatcctgcc
     5281 actccagcgc agtgaagtac ctcaccactc tatcttgacg gcgcgttata gtcttaaaat
     5341 cggcaatctc tgcgtaggaa acagcaaccg tttcgatatt cttttttctc acgcgctgta
     5401 taaagcgtaa aagccccgct tgattatcaa aaatgagctt tgccggaagt tggcggctaa
     5461 aattctttgc ggtatctgat gctccccaca aaaaaatacc aaagcctaaa agcatataag
     5521 cgacagtatt ttggggttct ccaagaacga ataacgcacc tgcgcctaaa aggatcaaag
     5581 caaaaatttt aaaaatcact cgaacgagca caaacccttt cgtctgctga aggattaaac
     5641 tttcgggtgt tatcgatcga atgcgaaaat tcataggttt tggggttgtc taaaaaatca
     5701 cgaccccact gaagaggtgc agtggcaatc aaaaacggac tagttcacgt tttcttgcgt
     5761 cggtgacttt tgggacaacc ccaattattc cctatatcaa ttcacgaaat tgtgatgcgc
     5821 tctacgacat cgtttttgtc aaatcgcgaa cctttttgga ggcatagttt tctgaaacaa
     5881 acgcccagtt cacgtgcgac caaaacgttt cgagatattt tgcacgtgcg ttgcggtaat
     5941 caacatagta cgcgtgctcc caaacatcgg cgacgagaag cggtgctggt ccattgttgg
     6001 tgaaaggcac ggccgcgttt gaagtcgaca caagagacaa cttacctgca gcgtcggcgc
     6061 acagccaaat ccaaccggag ccaaaagttt tcacgccacc gtcgacaaat tttgctttaa
     6121 gctcgtccaa actgccaaag tcgcgcttaa tcgcttctcc aagctctgcc gaaggagcgc
     6181 cgcctttacc ttgcgcagcc atattcaacc aataaaaagt atggttatac gcttgcgctg
     6241 cgttattaaa aataccgccc gatgaactca aaacaatttg ttcgagcgtc ttgcctttaa
     6301 gagcagcgtc gctttcgata aatttattca ggttgtcgat atacgcttta tgatgtttct
     6361 cgtagtggaa ggtcatttgc tcttcgttca aaaacggagc taaacctgtt ttcccatagg
     6421 gtaattctgg taatttaaac attgtactct cctttagttt attctaaact aattctaaaa
     6481 caagctaaaa tctaagttgc gccgtcaaat agaatacacc ttttgtcaaa aaacaccctg
     6541 tcccaacatt gcgtcggcga ctttaacaaa accgctgatg ttcgcgccgt cgacatagct
     6601 aatgtttcca tcgcggcgtt taccgtggag cacacaattt ttatgaatgc tcttcatgat
     6661 tccgtggagg cgtttatcta cctcttcttg cgaccatgaa agacgcattg cgttctgcga
     6721 catctccagt ccggaggttg ctacaccacc cgcattcgac gccttgcccg gcgcaaacag
     6781 tgtttttgaa ttttcaaaca cctcgacggc ttccagcgtg gaaggcatat ttgcgccttc
     6841 tgccacacag acgacaccgt tagcgactag cgctcgtgca tcgttctcgt caagttcatt
     6901 ttgtgtcgca cacggcaacg caatttcacc ggggatatgc catggccgtt tgcccgaaat
     6961 atattcaaaa cgtgcatctt cgcccaactc ggccaagcga ccgcgtcggt tatttttgag
     7021 gtccattacc gactgtatgg cttcgtcgtc aaaaccgtct tgggcgtaga gagtaccatc
     7081 cgaatcgcta aacgtaataa ccttaccacc caactcgcgc gctttgagtg cagcgtactg
     7141 cgccacattc cccgaaccgg aaatgagcac ccgcgcgcca tgcgtttccc gacgcgcttc
     7201 ctgcaacatt tcttcgacaa aatagactgt accaaacccc gttgcctcgg ggcggacgag
     7261 actaccgcca tagctcagtc cttttccggt aaacacacac gatgcttcgt tggtgagttt
     7321 cttcatcatc cctgccatgt agcccacttc gcggctgccg acgccaatat cgccggcagg
     7381 tacatcggta tccgaaccca agtgacggta taattctaac atcaacgctt gacaaaaccg
     7441 cataatctcg ccttcgcttt tacctttggg attaaaatca gaaccgcctt tcgccccccc
     7501 catcggcaac gttgtgagtg cattcttaaa tgtttgttcg aacgctaaaa attttaagat
     7561 tgaaagattc accgaaggat gaaagcgcat ccctccttta aaaggaccaa tcgcagaact
     7621 gtgctgcacc cgaaaagctc ggttgacttg aatctctcct ttgtcgtcgc tccacgcgac
     7681 acgaaattgg attgcacgct ccggttcaac gaggcgctct aaaagaccta actgtgcata
     7741 atgcgggtgc ctttctaaaa acggccaaag acttgctgtc acttcacgca cagcttgcaa
     7801 aaactctggt tgatgggggt cgcgtttttc gacgcgctgt aaaaactctt ctcggccggg
     7861 tagtttcaag tgttggtata catgagagca tgaaagtgcg cgtcaaatgc aaaactcttt
     7921 tgagctgtct aacccacgga catacctgac aaatgctacg ccgcaattgg ggggtattt
//
LOCUS       DAOMPD010000052         7827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_3995_length_7827_cov_2.663407, whole genome shotgun sequence.
ACCESSION   DAOMPD010000052 DAOMPD010000000
VERSION     DAOMPD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3995_length_7827_cov_2.663407"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="flgK"
                     /locus_tag="PLY93_02930"
     CDS             complement(<1..1159)
                     /gene="flgK"
                     /locus_tag="PLY93_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRP68461.1"
                     /translation="MASTFMGLEIARRGIAVHQQALTTTGHNISNADNKHYSRQRVDM
                     ETMHPLYELSMSRAAGAGHIGQGPKAGAVERVRDEFVDNRIHSAEQTKSQWETREKYL
                     HQIEVVYNEPSDDSLRAQLDRFWQSWQELSQFPEEMSHRELVRTRGREVTFRMRDTFQ
                     KLSDLRRQADYEFEVTVGRINDMGAQIRDLNERIMKAQAVGDRPNDLMDKRDQIVQEL
                     SKLADVTLEKQDPDEFIVYIGGEMLVQGSVQHKILLEGDASNEGMKKATWEHNGQKIL
                     FRAGSAQGLLEMRDITIRENIEKMDLLAVNLADTVNEVHRDGFGLTKETNINFFRIDP
                     LSRNIRGNYDLSGDGVDDVSAIFKVAGKNILTGDRPIGVAGTMTFTKIDAAH"
     gene            complement(1184..1720)
                     /gene="flgN"
                     /locus_tag="PLY93_02935"
     CDS             complement(1184..1720)
                     /gene="flgN"
                     /locus_tag="PLY93_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HRP68462.1"
                     /translation="MKTQTAPASAEKTASVGELVEVLRDLLGAHHELIRAEKEKQAAI
                     VARDGKKLKAATEAQTQELYVIELLESRRIQYSRQFFKNEKNYIDEKEIRLEAILSSE
                     NISAQEKKELTRYRDALKAALAELKRLSEINAQMLVDSRDLFKAMISSLTSKDSIATL
                     GNPTLATATRPVLIDANC"
     gene            complement(1777..2130)
                     /locus_tag="PLY93_02940"
     CDS             complement(1777..2130)
                     /locus_tag="PLY93_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68463.1"
                     /translation="MAAGIWTLLALAALALLFLTFRSGVPLYQKILAYVVVAALAYFY
                     WPEFMPTYQRVLGGDYKNIATELVAIVELALPTLAFALILSAFFTASALDAGKLLFLL
                     FILFLVAAVGKIVTL"
     gene            complement(2103..2999)
                     /locus_tag="PLY93_02945"
     CDS             complement(2103..2999)
                     /locus_tag="PLY93_02945"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRP68464.1"
                     /translation="MGDLFDSETTFVEKYFHTVDFSTKKDALDGFLSIKGRKKPLFLI
                     TGRGKNLEPGKANSLIALRSYHVFQKALKLFVGNPTASLVTLPVSKEFIMKAGVSFTG
                     HTEELAAAFQKSVFMCMYHKNFSVLPLTNHIALAQVPRKLYEVSLNALTDALRQFTAI
                     FEPKKGIAWCGVNPHCGEDGRVGTEEEFVRRGISFFENKSLRVDGPISADAVFTKRVR
                     DNYSLILANYHDQGLIPFKALAGLSGVNATLGLPRLRVSPDHGTAYSQTALKQVDTTG
                     VLASLQFALRYAKKWQQASGHF"
     gene            complement(3175..3639)
                     /locus_tag="PLY93_02950"
     CDS             complement(3175..3639)
                     /locus_tag="PLY93_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP68465.1"
                     /translation="MALVKSSQEITNSTIGPNSYFTGRFYINGSLKIDGKFEGRSLQA
                     DQLYIGTTGKVKTSVAANAVFVEGVIIGNIAAKSRVMLLPTAKIFGDIKTPELIIQNG
                     VILEGRCVIANDLKVSAKDVIESEYLKDNLNENVLFPKGARTEKEKEKIESR"
     gene            complement(3707..5230)
                     /gene="metG"
                     /locus_tag="PLY93_02955"
     CDS             complement(3707..5230)
                     /gene="metG"
                     /locus_tag="PLY93_02955"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRP68466.1"
                     /translation="MKKKIMITTPAYYVNARPHIGHAYTTIACDTWARYQRLAGNDVF
                     FLTGTDEHGDKIVKAAASHGVSVEAYCDEIAGAFKSVWQQLGIKFDDMIRTTEERHKK
                     VVQIILQKIYDAGDIYAGEYSGKYCFGCERYLTDKELNAKGECDVHLTVPEIISEKNY
                     FFRMQKYLPIWKDMLLKNRSYVAPDGYCNEVLGTIDELIKNGEDLSISRPKTRLKWGI
                     EIPFDKDYVTYVWFDALINYISAAAYGTPEFDERWAGAYHMIAKDIVKPHGVYWPTML
                     LAAQIPVYKGLVVHGYWLGFQDQKMSKSLGNAKDPLELASWLGNDALRFFLMREMNFG
                     SDARFSEEIMVNRLNANLANDLGNLVQRTLSMLKKYECGPNPNASFHKSQEAIHTALK
                     TLKVDYHRYFESFEFHNAIESVFVVIRLLNKIVDDYKPWQLAKENLAECASLLNTLLR
                     AIIVALLYLRPVLPEKTADLLALLQVTHDAEFPETLTAVHFADAKLDSWPMLFQRIE"
     gene            complement(5289..6050)
                     /locus_tag="PLY93_02960"
     CDS             complement(5289..6050)
                     /locus_tag="PLY93_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRP68467.1"
                     /translation="MYLKEKPKSRLKIDTRIFSESDLPRIYKAVRKYVSEGRQVYIIY
                     PVIDEVNDAEGNQTMASLMSEYAILEKEVFPDLRLGLLHGRLTGDEKDRAMQKFKEGL
                     IQVLVATTVVEVGIDVPNANVIVIRNPDRFGLSQLHQLRGRVGRGTHQSFCILVAPDH
                     ITSEGDARLQAMVKTDDGFELAEVDFQIRGAGEITGLKQSGASEFHTADLRVHGALME
                     KAVDFLQEHPQVQAQFAAMKNIKQTLEKGLVLFGN"
     gene            complement(6069..7358)
                     /locus_tag="PLY93_02965"
     CDS             complement(6069..7358)
                     /locus_tag="PLY93_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP68468.1"
                     /translation="MIPLTDTGLFSEIQLKTLARLKVKDVRDLFYYLPRKYIDRSQVL
                     NLRNSRSGDLVTVVGRIVHAELKFAGRKRFTIKIDANGFIVYATFFNGGVYLQKTLKV
                     GVEIALWGKLEVFRGRLSFMHPEWEIMSEESIHTGRIVPIYRITEAMRTSYLSVKALR
                     EKIEAALKRYEKRIVDYVPQETLQRNNLLSIQNALHKIHFPETIEETEAARKRLAFDE
                     LILFSVLTEEKKAAVRRIQKPFSLRLTREIQNEIKNLIRALPFELTHDQKRAVDTLLK
                     NSTQKFPVNHLLMGDVGSGKTLVALLTALFYMRHHLQVAYLAPTEILARQHYQTFLNI
                     LPGGGFEGVELVLGKENAKEKKAKLERLKRGESYLAIGTHALIQDDVAFKNLALLIID
                     EQHRFGVEQREALRKKGEHPDLLSMTATPIPRTLTLA"
     gene            complement(7364..7720)
                     /locus_tag="PLY93_02970"
     CDS             complement(7364..7720)
                     /locus_tag="PLY93_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68469.1"
                     /translation="MTKTLEVPLGEKILAAIDTDKKAKQQGYRDKIDKRWSVFIPSNK
                     NLINSIVASITRAIYCVPTEVLLGHEKGLKEDCCIDLINLHTVHRGRLYHFIGTLTLK
                     KTIGISCVLNIAMGCL"
BASE COUNT         1893 a         1872 c         1874 g         2188 t
ORIGIN      
        1 tgtgcgccgc atcgatcttt gtaaacgtca ttgtgccggc aacgccgatg ggacggtcgc
       61 cggtgagaat attttttcct gcgactttga aaattgcact gacatcgtcc acgccgtcgc
      121 cggataaatc gtaattgcca cggatgttgc gcgaaagagg atcgatccga aaaaaattga
      181 tattagtttc ttttgttaaa ccaaaaccgt cgcggtgtac ttcgtttacc gtgtctgcta
      241 agttgaccgc gagtaaatcc atcttttcga tattttcgcg gatggtaatg tcgcgcattt
      301 caagaagacc ttgagcgctg cccgcacgaa ataaaatttt ttgaccgttg tgctcccacg
      361 tcgccttttt cataccttcg tttgaagcgt cgccttcgag taaaatttta tgttgcacgc
      421 tgccttgcac caacatctca ccgccgatgt agacgatgaa ctcgtctgga tcttgtttct
      481 caagcgtcac gtcggcgagt ttggagagct cttgcacaat ttggtcgcgc ttatccatga
      541 ggtcgttggg gcgatcgccc accgcctgcg ctttcatgat acgttcgttt aagtcacgga
      601 tttgcgcgcc catgtcgttg atgcgaccga cggttacttc aaattcatag tcggcttggc
      661 ggcgcaagtc ggaaagtttt tggaatgtgt cgcgcatgcg gaacgtgact tcacggccac
      721 gggtgcgcac cagttcacgg tggctcatct cttcgggaaa ttggctcagc tcctgccacg
      781 attgccagaa gcgatcgagt tgcgcacgca acgaatcgtc ggagggttcg ttatacacaa
      841 cttctatttg gtgtaaatat ttctcgcgcg tttcccattg cgatttagtt tgttcggcag
      901 agtggatgcg attatcgaca aactcgtcgc gcactcgctc cacagcgcct gctttaggtc
      961 cctgtccgat atgccctgct cctgcggcgc ggctcatcga cagttcataa agagggtgca
     1021 tcgtttccat atcgacgcgt tgccgtgaat agtgtttgtt atccgcgttg gagatgttgt
     1081 gtcctgtggt ggtgagcgcc tgttgatgca ccgcgatgcc gcgtcgtgca atctctaagc
     1141 ccatgaaggt ggatgccata ttttctcctt aactaaatct ccgttaacaa tttgcgtcga
     1201 tgagtacagg tcgcgttgct gtcgccaaag tgggatttcc caaggtggcg atagagtctt
     1261 tggaagttaa gctggaaatc atcgccttga aaagatcgcg gctatcgacg agcatctgtg
     1321 cattgatttc tgaaagccgc ttgagctccg ccagtgcagc cttgagtgcg tcgcggtaac
     1381 gcgtgagttc ttttttttcc tgtgcagaaa tattttcgct tgatagaatt gcttctaaac
     1441 ggatttcttt ttcatcgata taattttttt cgtttttgaa aaattgccga ctatactgta
     1501 tgcggcgact ttcgagaagc tcaatcacat agagttcttg agtttgtgcc tcggtggcgg
     1561 ctttgagctt ttttccgtcg cgggcaacga ttgccgcttg cttttctttt tcagctcgaa
     1621 tgagttcgtg gtgggcaccg agtaaatcgc gcaatacctc gactagttca ccgaccgaag
     1681 cggttttttc tgccgacgcg ggcgcagtct gggttttcat attttaagca tcggcaaaaa
     1741 actaaaaagc atgagtgaat tgagggcagc atctttttaa agagtgacaa tcttacccac
     1801 ggcggcgact aaaaacaata taaaaagaag gaacaacaat ttgcctgcat ccaacgctga
     1861 tgcggtaaaa aaagcgctca ggattaacgc gaaggctaac gtaggtagcg ccaactcaac
     1921 aatggcgact agttctgttg cgatgttttt atagtcaccg cctaaaacgc gttggtacgt
     1981 gggcataaat tcaggccagt agaaataagc gagcgcagcc accacgacat acgccaaaat
     2041 tttttgatag agggggacac cgctgcgaaa agttaaaaac aacagcgcga gcgctgcgag
     2101 cgctaaaagt gtccagatgc ctgctgccat tttttcgcgt acctcaatgc aaattgtaaa
     2161 ctcgcaagca ctcctgtcgt gtctacttgt tttagagccg tctgcgagta ggcggtgccg
     2221 tgatcgggag aaacgcgcag gcggggaagc cccaacgtgg cgttcacacc cgataacccc
     2281 gctaatgctt taaaagggat taacccttgg tcgtgataat tggcgaggat gagggaataa
     2341 ttatcccgta cccgttttgt aaaaacagcg tcggcggaaa ttggcccgtc gacacgtagg
     2401 cttttgtttt caaagaaaga aatcccccgc cgcacaaatt cttcttcggt gcccactcgc
     2461 ccgtcttcgc cgcaatgggg gtttacaccg caccaggcga tgcctttttt aggctcaaaa
     2521 attgcagtga attggcgcag cgcgtcggtg agcgcattca gtgaaacttc gtaaagtttg
     2581 cgcggcactt gcgcgagtgc aatgtggttg gtgagcggca gtaccgaaaa atttttatga
     2641 tacatacaca taaaaacaga tttttgaaat gccgccgcaa gctcttcggt gtgaccggtg
     2701 aagctcaccc ctgctttcat aataaattct ttagagacgg gtagagtaac caacgacgct
     2761 gtgggatttc ccacgaatag ttttaacgcc ttttgaaaaa catgatacga acggagcgcg
     2821 atgagagaat tggctttgcc gggttcgagg tttttacctc gtcccgtgat aagaaagagc
     2881 ggtttcttgc ggccttttat cgacagaaag ccgtctaatg catccttttt tgttgaaaaa
     2941 tcaacggtgt gaaaatactt ctcgacaaat gttgtttcag aatcgaataa atctcccaca
     3001 acgacaaagc gtttgagtgg cgagacattt ccttgagagg tgagtctata ttctttgacg
     3061 tgactaatga tttgtttaaa cgcaaaccat cccgcaccgg cagcatcgcc ttgcgagatg
     3121 ataataggtt caataggggc tgtagcggaa atgcttaacc aaccttactt aaacttagcg
     3181 tgattcgatt ttttcttttt ctttctcggt gcgtgcacct ttggggaaaa gcacattctc
     3241 gttgagattg tcttttaagt attcagattc aatcacatct ttggcagaaa cttttaagtc
     3301 attggcaata acgcaacggc cttcgagaat cacgccgttt tggatgatga gttcgggagt
     3361 tttgatgtcg ccaaaaattt ttgctgtggg aagtagcatc acgcgacttt tcgcagcgat
     3421 gttgccgata atcacccctt cgacaaaaac agcgttggct gccacggagg tttttacttt
     3481 gccggttgtg ccgatgtaaa gttgatcagc ttggagcgaa cggccttcaa acttaccgtc
     3541 aattttgaga cttccgttga tataaaaccg accggtgaaa tacgagttgg ggccgatggt
     3601 tgaattagtg atttcttgag agcttttaac gagagccatg ggttaccctg catttgccgc
     3661 ataagctgtc atgcgtttct tataaaacat gcccgcgcac agactcctat tcaatccgct
     3721 gaaagagcat cggccaggag tctaacttcg cgtctgcaaa atgtactgcc gtgagtgttt
     3781 ctggaaactc tgcatcgtgt gttacttgaa gaagagcgag taaatccgcc gttttttctg
     3841 gcaataccgg gcgcaaatac aagagcgcta caataattgc gcgcaatagc gtgttgagga
     3901 gcgatgcaca ctcggcaaga ttttcttttg ccagttgcca cggtttgtaa tcgtcaacga
     3961 ttttattcaa caggcggatt accacaaaaa cgctctcaat cgcattgtgg aactcgaacg
     4021 attcaaagta gcggtggtaa tcgactttaa gtgttttaag cgctgtgtgg attgcctctt
     4081 gacttttgtg aaagcttgcg tttgggttgg ggccgcattc gtacttttta agcattgaga
     4141 gcgtacgctg taccaaattt cccaaatcgt tggccaagtt agcgttcaaa cggttgacca
     4201 taatctcttc ggagaaacgc gcatcgctac cgaaattcat ttcacgcatc aaaaaaaagc
     4261 ggagtgcatc gttaccaagc catgacgcca actccaatgg gtcttttgca tttcccaaac
     4321 tttttgacat tttttggtct tgaaaaccca accaataccc atgtaccacc aaacctttat
     4381 agacggggat ctgcgccgcc aacaacatcg tcggccaata gacaccgtgg ggtttaacga
     4441 tgtcttttgc aatcatgtgg tatgcgcccg cccagcgttc gtcgaactcc ggtgtaccgt
     4501 aagccgccgc gctgatatag ttgatgagcg cgtcaaacca cacgtaagtt acatagtctt
     4561 tgtcgaatgg gatttcaatc ccccacttga gacgcgtctt ggggcgtgaa atcgacaagt
     4621 cctcgccgtt cttgatgagc tcgtcgatgg tccccaaaac ttcgttacag tagccgtcgg
     4681 gggcgacgta gctgcggttc ttgagcagca tgtctttcca aatcggtaaa tatttctgca
     4741 tacgaaaaaa ataatttttc tctgaaataa tttcaggcac ggtgagatgg acatcgcact
     4801 cgcccttagc gttcaactct ttgtcggtga gataacgttc gcaaccaaag caatacttcc
     4861 ccgagtattc acccgcgtag atgtcacccg cgtcgtaaat tttttggagg ataatttgca
     4921 ccactttctt gtgtcgctct tcggtggtgc ggatcatgtc gtcgaattta atgcccagct
     4981 gttgccacac gcttttgaac gcgcctgcaa tttcatcgca gtacgcctcg acgctcacgc
     5041 catggctcgc cgcggcttta acaattttat cgccgtgctc atcggtgccg gtgaggaaaa
     5101 aaacatcgtt gcccgccaag cgttggtaac gcgcccacgt atcgcaagca atggtggtgt
     5161 acgcatggcc gatgtgtggt cgcgcattca cataatacgc gggggtggtg atcattattt
     5221 tctttttcat gaaaattaaa caaatttaca aatcactcaa aagtcagcgc gtcaatcgtt
     5281 aagtattttc aattcccaaa aagcaccaac cctttttcta acgtctgctt gatatttttc
     5341 atcgcggcaa attgcgcttg cacttgcgga tgctcttgta aaaaatctac cgccttttcc
     5401 atgagcgcgc cgtgtacgcg taggtcggcg gtgtggaact cggatgcccc cgattgttta
     5461 agaccggtaa tttcacccgc accacggatt tgaaaatcta cctctgcaag ttcaaagcca
     5521 tcgtcggttt tgaccatcgc ttgcaggcgg gcatctccct ctgaggtgat atggtcgggc
     5581 gccaccaaaa tgcagaaact ttggtgtgtg ccgcgcccca cacgcccgcg caactggtgc
     5641 agctgcgaaa ggccaaagcg gtcggggttg cgtatcacaa tgacgttcgc gttgggtacg
     5701 tcgataccta cctcgaccac ggtcgttgcc accaatactt ggatcaatcc ctctttgaat
     5761 ttttgcatcg cgcggtcttt ttcatccccc gtgagacgcc cgtgcaaaag tcccaaacgt
     5821 aaatcaggga aaacttcttt ttctaaaata gcgtactcgc tcatgagcga tgccatcgtt
     5881 tgattcccct ctgcgtcgtt cacttcgtcg atgacaggat agatgatata gacttgccgc
     5941 ccttcgctaa cgtactttcg taccgccttg taaatacggg gcaagtcgct ttctgaaaaa
     6001 atacgcgtgt caattttaag gcgacttttc ggtttttctt tgagatacaa cggttcaagg
     6061 tcgccgtact acgcgagcgt caacgtacgc ggtatcggtg tggcggtcat cgacaacaaa
     6121 tcggggtgct ctcctttttt gcgcaacgcc tcgcgctgtt ccacaccaaa gcggtgttgt
     6181 tcgtcaataa tgagaagcgc taaatttttg aacgccacat cgtcttgaat aagcgcatgc
     6241 gtaccaatcg caagatatga ttcgccacgc ttgagacgtt caagcttggc ttttttttct
     6301 ttcgcattct ctttaccgag taccagctcg acgccttcaa aaccaccgcc cggcaaaatg
     6361 ttcaaaaatg tctggtagtg ttgccgcgct aaaatttcgg tgggggctaa atacgccact
     6421 tgcaaatgat gccgcatata aaaaagggcg gttagaagtg cgacgagggt tttaccgctc
     6481 cctacatccc ccatgagtag atggttgacg ggaaattttt gtgttgaatt tttgagtaat
     6541 gtatcgactg ctcttttttg atcgtgcgtg agttcaaaag ggagcgctcg aatcaaattt
     6601 tttatttcat tttggatttc acgcgtgagg cgtaagctaa acggtttttg aatgcgccgc
     6661 acagctgcct ttttttcttc ggtgagaaca gaaaataaaa tgagctcgtc gaacgccaaa
     6721 cgtttgcgtg ctgcttcggt ttcttcgata gtttcaggaa aatgaatttt gtgtaacgca
     6781 ttttggatgg agagcaaatt attcctttga agcgtttctt gcggcacata atcgacgatg
     6841 cgtttttcat aacgctttag agccgcttca attttttcgc gcagcgcctt taccgataaa
     6901 tacgatgtcc gcatcgcctc ggtgatgcgg tagatgggta cgatgcgccc ggtgtggatg
     6961 ctctcttcgc tcatgatttc ccattcgggg tgcataaaac tcaagcggcc acggaaaact
     7021 tcgagctttc cccagagcgc aatctcaaca cccactttga gcgttttttg tagatagact
     7081 ccaccgttaa aaaacgtggc atagacaatg aaaccgttgg cgtcgatttt gatggtgaag
     7141 cgcttgcgcc ccgcgaattt taattccgca tgcacaatac gccccaccac ggtgaccaaa
     7201 tcccccgagc gcgaattgcg caaattcaaa acttggctgc gatcgatgta cttgcgcggt
     7261 aaataataaa ataaatcgcg cacgtcttta actttaaggc gagcgagtgt cttgagttgg
     7321 atctcgctaa aaagacctgt atccgtgagg ggaatcacaa caactacaaa caacccatcg
     7381 caatgttcaa gacgcagcta atgcctatcg tttttttcag cgtgagcgtg ccgatgaagt
     7441 ggtatagtcg ccctcggtga acggtatgca aattgattaa gtcgatacag caatcttctt
     7501 taagaccttt ttcatgacca agtaatacct cagtgggtac acagtaaatc gcacgcgtga
     7561 tactcgcaac aatactattt attaaatttt tatttgaagg aataaatacc gaccatcgtt
     7621 tatctatctt atctctatac ccctgttgtt tcgctttttt atcggtgtcg atagccgcca
     7681 gtattttttc gcctaaagga acctcaagcg tcttggtcat gctcgacttt agagggattt
     7741 gtatgcggtg tcaagcgtat ctgcccgctt aatttatgca acccacagag ataactgaca
     7801 cttttggggt caagttgagt gcgtttt
//
LOCUS       DAOMPD010000053         7746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4046_length_7746_cov_3.019373, whole genome shotgun sequence.
ACCESSION   DAOMPD010000053 DAOMPD010000000
VERSION     DAOMPD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4046_length_7746_cov_3.019373"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLY93_02975"
     CDS             <1..354
                     /locus_tag="PLY93_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRP68470.1"
                     /translation="ENIYRFEKIYVLIGPQTYSAAVYFARLLKLANSHVTLVGTQTGS
                     PGDGHSAETLVTYRLPESGIFFDVPLARVEFSPLVPGQVKGQGLMPDKEVKETLDDFR
                     FGRDTVLETVVSYPH"
     gene            369..1991
                     /locus_tag="PLY93_02980"
     CDS             369..1991
                     /locus_tag="PLY93_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRP68471.1"
                     /translation="MRHSFDKLFFLVLFFLLQCKSDLIKDRPISFSSWRKQQTINYIR
                     AHYGTDAQDIKMLPVMVVSHYTAMDSTDVSFEYMNKEEMEAGRKVLKDAGLNNIAVHF
                     LVGKDGTIYRLMPENHIGRHVIGLNRHAIGIENVGKDESALTQAQVDANAYIVRELVK
                     KYPIHYLIAHSEYRNFEKTPLWEEKDDAYRTKKIDPGESFMRSLREKLKDLNLKSQYD
                     GSELYDRLGYTLDNYFRKGEFEGNALIIFNGKTILEKAYGKQKITDEIYLASAAKPIT
                     AAAVYALISEKKLSLKTPVAPYFPTLKKILPNVQVRHLLNHTSGIEDYYKLTEEKGPF
                     TNSAVLAFLETMEKPLFKAGKKFYYANSNYILLAELIEKITNKKFTEYVREKIFIPAK
                     METSFFVTEIVPEKKIITATTASGDSLLYPHKTVGAGGLYATTADLARFGMALNQDAF
                     FNHALFLKMVEPSTSVEQKKTQYASGWYVYPKQGIIYHDGNFNGYHTINYLEWKKKNV
                     IILLARHSTNKIKEITYELGMAINGRTAKKLE"
     gene            complement(2010..2369)
                     /locus_tag="PLY93_02985"
     CDS             complement(2010..2369)
                     /locus_tag="PLY93_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRP68472.1"
                     /translation="MGAVLLLVCSNIFMTLAWYGHLELHKSAKYSSLPLYAVIFFSWF
                     VALAEYALQVPANRIGSENYGGPFNLFELKVIQEVISLVVFTFFAVYVFKSDTLKWNY
                     VVGFFFLVLAVILIFKK"
     gene            complement(2414..2818)
                     /locus_tag="PLY93_02990"
     CDS             complement(2414..2818)
                     /locus_tag="PLY93_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HRP68473.1"
                     /translation="MKRVFTFTVMVALLVLGCNKQSVYKGRVWFGEPLDKAEVTSPVK
                     FTMHVEGMKIKPAGEMELGTGHFHILINKEAVPAGDVVVTDPVHIHYGKGQTEDTRAL
                     EKGDYSITLQFADGAHVSYGPMWAQTIHIKVK"
     gene            2920..3351
                     /locus_tag="PLY93_02995"
     CDS             2920..3351
                     /locus_tag="PLY93_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68474.1"
                     /translation="MQRAVYILTTAAKKTVLGSFLTLFIFSNAVVGIDLTPEIRHDAK
                     TEILSSALVSSSHSFLPNGFHESSSLEIQRLEYNLLRQIMPRHGIERAWHWQINAFDG
                     SRENKILISADYALLCSNLFSLAHTRLDSRYPIPPPTKQNG"
     gene            3409..4791
                     /locus_tag="PLY93_03000"
     CDS             3409..4791
                     /locus_tag="PLY93_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP68475.1"
                     /translation="MRVGFCVNVNKPQGGSLLKKIFFVSVVTTIALWPQNNLLTEASP
                     DIPRKKFRVTLAEVEKSFVEKNLSLLAARFQIDAKKALVMQAGLYANPSVFLETNIYN
                     PRTKQFLDATENGSQVIQIQQLFLLGGKIEKRVKVAEIDKALSENDFFNVLRALKYEL
                     RKTFFSLYFERESILYYDQTIEALMKTVKGAEVALSRRAITKAEYLRLKALSYFLRNE
                     RANILVDLRLKEVNLRVLMNRPDLADVEIVPEVNETELEATAIGSLTADQLLKEAEEH
                     RPDIKAAMLQLKYETANLDLQEANAIPDLAFGPVFNRANTYIPNYWGITAQVSIPLFD
                     RNQGNIEAAKKAIITRKQQLLNERMKLEGELSASLIKAKEKDKIYQEFKKGHVQDFRD
                     LMNVMVLSYQKRYISVIEFADFFETYRNSIVQLIDLKTQRTTALENINYAIGKDILFL
                     QTPTAATEEK"
     gene            4865..5386
                     /locus_tag="PLY93_03005"
     CDS             4865..5386
                     /locus_tag="PLY93_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68476.1"
                     /translation="MKLLCIFLSIGLLSAAEASQVASDKFTQASQDAGEAVERNRVTN
                     SFFAIGPTIGSPAGLNAHASLYLWRFVVHGSGMFYSPDFMGTQGGVGFSFLNGARIRH
                     SVSFVGGFMRRNPVFVNSLGGVVQDAAYAGGAYEFFADGFFLQVGLAHGLSGSMANPI
                     LIFQTGYLFSFMD"
     gene            5404..5694
                     /locus_tag="PLY93_03010"
     CDS             5404..5694
                     /locus_tag="PLY93_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68477.1"
                     /translation="MKVKLTILASVVALWACTAKAQSATKYVAPVVKDNGAVVYFPPG
                     AQGRETISTRTFGEGKEFIQIQAPAHIVATVGHSVSGGGRIVLFESADLNTA"
     gene            5769..6359
                     /locus_tag="PLY93_03015"
     CDS             5769..6359
                     /locus_tag="PLY93_03015"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP68478.1"
                     /translation="MQVATERDIIDAETEAGNSSAAVAESEGKLRAAGVNPADLNRYG
                     GHIAWLIADIGESRFETTKSGKPVNIIFNSFPDKKFKGKVQAVGDNIDPQTRTVKVRI
                     VLKNPNNKFKPGMFANVEFLDDAQAPMVLPFNSVFTVEGENFVFVEEKKDAFRKKFVV
                     LGNSNVNYVEILSGLSKGDMVATEGVLFLKGLSVGF"
     gene            6381..>7746
                     /locus_tag="PLY93_03020"
     CDS             6381..>7746
                     /locus_tag="PLY93_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP68479.1"
                     /translation="MIFKTIEFALKHKLPTLALAFVIFALGVWSWVGIQKEAYSDIAD
                     TQVRLVVAFPGRAAVEVEERVTLPIERVLNNTPNVRVRRSRTINGLVVFQFVFEEGTD
                     DYFARIRLMEKVKEADIPPEVEPELAPMSSPSGEIYRYILETRANHTPMEVRTAQDWI
                     VMPKILGVPCIADVVTFGGLEKQYHVITTPERLVQYNTNLDEVVGAIQSNNLNTGGNI
                     LRQGDQGFAIRSLGAIRSIQDIKNVVVKVQNGVPVFVKDLASVEVSPLIPQGYITYFT
                     QNAEEGEFRSTSGVQGNIAMRRWCDPNVTGENIKAKVKEINEQYLPKGMEIKPMVDRA
                     NLVDYTLRTIGSTLIEGVTIVTLILIFFIGSVRTAMVVVLTIPFSLLFAFILMRMTGL
                     SANLLSLGAIDFGIIVDGVVIMAENIMRSYREKRENRHLKLDEHTLNSSKEVGMEIFF
                     SILII"
BASE COUNT         2247 a         1762 c         1690 g         2047 t
ORIGIN      
        1 gaaaatatat accgttttga aaaaatatac gtccttattg ggccgcaaac atattcggcg
       61 gcggtgtatt ttgcgcggct tttaaaactg gcaaatagtc acgttaccct tgtaggtacc
      121 caaacaggta gtccggggga cgggcatagc gcagaaactc tcgtcacgta ccgtcttcct
      181 gaatcgggaa tttttttcga tgttcccctt gcacgtgtcg aattttcgcc tcttgttcct
      241 ggccaagtta aaggccaggg acttatgccc gataaagaag ttaaagaaac cctcgacgat
      301 tttcgctttg ggcgcgacac cgttcttgaa acagttgttt cgtatcctca ttgatactgg
      361 tatagagaat gcgccacagt ttcgataaat tattttttct tgtgcttttt tttcttctcc
      421 agtgtaaatc cgaccttata aaagatcgcc ctatttcttt ttcttcttgg cgaaagcagc
      481 agacaataaa ttatattcga gcgcattacg gtacggatgc acaggatatt aaaatgcttc
      541 ctgtaatggt cgtgtcgcat tatacagcga tggactccac cgatgtttcc tttgaatata
      601 tgaacaaaga agaaatggaa gctggacgaa aggtattaaa agatgcgggg cttaacaata
      661 ttgccgtgca ttttcttgtg ggaaaagatg gaacaattta ccgtcttatg ccagaaaacc
      721 atattggccg gcatgttatt ggactaaacc gccacgcaat tgggattgaa aatgtcggta
      781 aagatgaaag cgcacttacg caagcgcagg ttgatgcaaa cgcgtacatt gtgcgcgagc
      841 ttgttaaaaa atatcccatc cattatctta ttgcacactc cgaataccgc aactttgaaa
      901 aaacgccgtt gtgggaagaa aaagacgacg cataccgtac gaaaaaaatt gaccccggag
      961 agtcttttat gcgttccttg cgggaaaaac ttaaagattt aaaccttaaa agccaatacg
     1021 acggaagcga gctttacgac cgccttggct atacactgga taattatttt cgaaaaggag
     1081 aatttgaagg aaacgcatta atcatcttta atggaaaaac tattttggaa aaagcgtacg
     1141 gcaaacaaaa gataaccgac gaaatttatc tggcttcagc ggcaaagcca attaccgcag
     1201 cggcggttta cgctttaatt tcagaaaaaa aactttcgct taaaactccc gtagctcctt
     1261 attttcctac attaaaaaaa attttaccca atgtccaagt acgccacctt cttaaccaca
     1321 cttcgggcat tgaagactat tacaaactta ccgaagaaaa aggacctttt acaaatagcg
     1381 cggtacttgc ttttttagaa actatggaaa aaccactttt taaagcaggt aaaaaatttt
     1441 attatgcaaa ttcaaactat attctacttg cagaacttat cgaaaaaatt acaaacaaaa
     1501 aatttacgga gtacgtccgt gaaaaaattt ttattcccgc aaaaatggaa acaagttttt
     1561 ttgttaccga aatagttccc gaaaaaaaaa ttattacagc aactaccgcg tcgggagata
     1621 gcttgcttta cccccacaaa acagttggag caggcggcct ttatgcgacc acggccgatt
     1681 tggcgcgttt tggtatggcg cttaaccaag acgcattttt taaccacgcc cttttcttaa
     1741 aaatggttga accttcaaca agtgtcgaac agaaaaaaac acagtacgca tcgggatggt
     1801 acgtgtaccc caagcaaggc attatttacc acgatggaaa ttttaacggt taccatacca
     1861 tcaattattt ggagtggaaa aaaaagaatg ttatcattct gcttgcacgc cattcaacaa
     1921 acaagattaa agaaattacg tacgaacttg gtatggccat taacggccgt acggcaaaaa
     1981 agctggagta gaggtggtta aaattactct tacttcttaa aaatcaaaat aactgcaagc
     2041 actaagaaga aaaacccgac aacgtaattc catttgagcg tatcgctttt aaaaacataa
     2101 actgcaaaga aggtaaatac tacaagggaa attacttctt ggataacttt aagctcgaaa
     2161 agattaaaag ggccgccgta attttcacta ccgatacggt ttgcaggaac ctgtaacgcg
     2221 tattcagcaa gcgcaacaaa ccaactaaaa aatatgacag catagagggg aagcgacgaa
     2281 tactttgcgc ttttgtggag ttccaaatgt ccataccaag caagcgtcat gaatatattc
     2341 gaacagacga ggagtaaaac tgcgcctata ccgtgcgaca cttaaatttt atttaaaagt
     2401 ttctgttaaa ttgctattta actttaatgt gtattgtttg tgcccacata gggccatacg
     2461 aaacgtgtgc gccatcggcg aattgtagcg taattgagta atcgcctttt tcgagtgcgc
     2521 gtgtgtcttc tgtttggccc ttaccgtagt ggatgtggac agggtcggta acaacaacgt
     2581 cgccagcagg tactgcttct ttgttaatga ggatgtggaa gtgtccggtt cccagttcca
     2641 tttctccagc aggttttatt ttcatacctt cgacgtgcat ggtaaatttc acaggagacg
     2701 ttacctcggc tttatctaaa ggctcaccaa accaaaccct tcctttgtag acgctttgct
     2761 tattgcaacc aagcaccaaa agtgcaacca ttacggtaaa ggtaaaaacg cgtttcatac
     2821 atccgccttt tgtggccttt tagagttgta aagttaattg aggaaatccc tttcactctg
     2881 gttgacaacg tgagggttta acgaaggtgg caccagcaaa tgcaaagggc tgtttacata
     2941 cttacaactg ctgcaaaaaa aaccgtactg ggttcttttt taaccctatt tattttctcc
     3001 aatgcggttg tgggcattga tttaacgccc gaaatacgcc acgatgctaa aacagaaatc
     3061 ctctcttctg cccttgtttc ttcaagccac tcgtttctac ccaacggttt ccacgaaagt
     3121 tcttcgttag aaatccaacg tttagagtat aacctgctac ggcaaataat gccacgccat
     3181 ggcatcgaac gtgcttggca ttggcaaata aatgcgttcg atggttcgcg tgaaaataaa
     3241 attttaattt cggccgatta cgcccttctt tgttcaaatc ttttttctct tgcgcatacg
     3301 cgcctcgatt ctcgctaccc cattccccct cccacgaaac aaaacggata actttaaagt
     3361 tatccgctaa gccaagaaag tatttggcgt gtgctttgcg ctacgcctat gcgcgtgggg
     3421 ttttgcgtaa atgttaataa accgcaaggg ggaagtttgc taaaaaaaat attttttgta
     3481 agtgtggtta ctacaatagc attgtggcca caaaataatt tgctcacaga ggcttctcca
     3541 gatataccgc gcaaaaaatt tcgtgtaacc cttgcagagg tagaaaaatc ttttgtggaa
     3601 aagaacctct cgcttttggc tgcacgtttc caaatcgacg ccaaaaaagc tcttgttatg
     3661 caagcagggc tttatgcaaa cccaagcgta tttcttgaaa ccaacattta caatccacgg
     3721 acaaagcaat tcttagatgc cacagaaaac ggaagccaag ttatccaaat acaacagctt
     3781 tttcttttag gcggtaaaat tgaaaaacgc gttaaggtcg ccgaaatcga taaagcgtta
     3841 tccgaaaatg atttcttcaa cgttttacgt gcactaaaat acgaactacg aaaaactttt
     3901 ttctctcttt attttgagcg tgaaagtatt ctttactacg accaaactat cgaagctctc
     3961 atgaaaaccg tcaaaggagc ggaggttgcg ctttcgcgcc gcgcgatcac caaagccgaa
     4021 tatctgcgcc tcaaggcatt gagttatttc ttgcgcaacg aacgcgcaaa tattctcgtc
     4081 gatttacgct taaaagaagt gaatctgcgt gtgctcatga accgtcccga tttggcggat
     4141 gtagaaattg tacccgaggt gaacgaaacg gagttagaag cgaccgctat tggttcgctc
     4201 acggccgatc aacttcttaa agaagccgaa gaacaccgcc ccgatattaa agccgctatg
     4261 ttgcagctca aatacgaaac cgccaattta gatttacaag aagcgaacgc gatacccgac
     4321 ttagcttttg gccctgtatt taatcgtgcg aatacttata ttcccaacta ctggggtatt
     4381 accgcgcagg taagcattcc actttttgac cgcaaccaag gcaatatcga agcagcgaaa
     4441 aaagccatca taacgcgcaa gcaacaactt ctgaacgagc gcatgaaact tgaaggagag
     4501 ctgtcggcgt cgctcatcaa agcaaaagaa aaagataaaa tttatcaaga attcaaaaaa
     4561 ggacacgtac aagactttcg tgatttgatg aacgtgatgg tgttgagcta ccagaagcga
     4621 tatatctcgg tgattgagtt tgccgacttt tttgaaacct accggaacag cattgtgcaa
     4681 ctcatcgatt tgaaaacaca acgcactacc gcattggaaa acatcaacta cgccatcggc
     4741 aaagacattt tatttttaca aacgccgacc gctgcaacgg aggaaaaatg actaaacctg
     4801 cccccccccc cccccccccc cccccccccc ccccccagtg cgagtgtgga gaggggcttg
     4861 gaggatgaag ctcctctgta tctttctatc tattggcttg ctgagcgcag ccgaagcaag
     4921 ccaagtagca agcgataaat ttacccaagc atcccaagac gccggtgaag cggtcgagcg
     4981 caaccgtgtc accaattcat tctttgccat tggcccaaca attggttcgc ctgcgggact
     5041 aaacgcacat gcatcgctct atttgtggcg gtttgtcgtg cacggttcag ggatgtttta
     5101 ttcccccgat ttcatgggga cgcaaggcgg cgtcggtttt tcattcttga acggcgcacg
     5161 tatccgccat agcgtttcgt tcgtcggcgg ttttatgcgc cgtaatccgg tttttgtaaa
     5221 tagccttggt ggtgtagttc aagacgcagc ttatgcgggc ggtgcgtatg aattttttgc
     5281 ggacggcttt tttttacaag tgggtctcgc gcacggcctg tcgggaagta tggccaaccc
     5341 gatactcatt tttcaaaccg gttatctatt ttcttttatg gattaaaaaa taaggagaaa
     5401 acaatgaaag taaaattaac aatcctcgcg agcgttgttg cactgtgggc gtgtaccgcc
     5461 aaagcgcaat ccgcaactaa gtacgtcgcg cctgtcgtta aagataacgg tgctgtagtt
     5521 tattttcccc ccggtgcgca aggccgtgaa acgattagca cgcgaacttt tggcgaaggc
     5581 aaagaattta ttcaaattca agcgcctgcg cacatcgttg ctactgtggg acattcggtt
     5641 tcgggcggtg gccgtattgt gctttttgaa tcagccgact taaacaccgc gtagcgacat
     5701 accgccaatc gcgcaacgct tcgtctcggg cacacaagaa cctcaaacgg ttgagagaca
     5761 tgtattcgat gcaagtggct accgagcgcg acattatcga tgcggagacc gaggccggca
     5821 attcgagcgc cgccgtcgcc gagtcggaag gaaaattgcg ggcggcaggg gtaaatcccg
     5881 ctgacttgaa tcggtatggc ggtcacattg catggcttat tgccgacatc ggtgaaagcc
     5941 gttttgaaac aacgaagtcg ggtaaaccgg tgaatattat ttttaactca ttccccgata
     6001 aaaaattcaa aggcaaagta caagcggtgg gagataacat cgatcctcaa acacgcacgg
     6061 ttaaagtgcg cattgtctta aaaaatccca acaacaaatt taaacccggc atgtttgcca
     6121 acgtcgagtt tttagacgac gcacaggcac cgatggtttt accgttcaac tctgttttca
     6181 ccgttgaagg ggaaaacttt gtctttgttg aagaaaaaaa agatgcgttt cgtaaaaaat
     6241 ttgtcgtctt gggtaattca aatgtcaatt acgttgaaat tttaagtgga ctttccaaag
     6301 gcgatatggt tgcaaccgaa ggcgtgctct tcttgaaagg cctttcggtc ggtttctaaa
     6361 gcgacggcgt tatgcaaaaa attatcttca aaacgattga gtttgcatta aaacacaaat
     6421 tgccaacatt ggcgttggcg ttcgtcattt ttgcactcgg cgtgtggtca tgggtgggca
     6481 tccagaaaga agcgtactcg gacattgctg acacgcaagt gcgtcttgtg gtcgctttcc
     6541 ccgggcgcgc cgcagtcgaa gtggaagaac gcgtcacatt gccgattgag cgtgttttga
     6601 acaacacacc caacgtgcgc gtgcggcgtt cacgcacgat taacggcctt gtggtttttc
     6661 aatttgtttt tgaagaaggg acagacgatt attttgcgcg tatacgcctt atggaaaaag
     6721 tgaaagaggc agatattcct cccgaggtgg agcccgaact tgcgccgatg agctcaccct
     6781 cgggtgaaat ttatcgctat attctggaaa cacgcgccaa ccacaccccg atggaagtac
     6841 gcaccgcgca agactggatt gtgatgccta aaattttagg cgtgccttgt atcgccgatg
     6901 ttgtgacgtt tggtggcttg gaaaaacaat accacgttat caccaccccc gaacgattgg
     6961 ttcaatataa caccaacctt gacgaagtgg tcggtgcaat tcaaagcaat aacttaaaca
     7021 cgggcggcaa tattttgcgc caaggcgatc aaggatttgc tatccgctcc ttaggcgcca
     7081 ttcgttcgat tcaagatatt aaaaacgtcg ttgtaaaagt gcaaaacggc gtacccgttt
     7141 ttgtcaaaga ccttgcatcg gttgaagtct ctccgcttat accacaaggg tacatcacct
     7201 actttactca aaacgccgaa gaaggcgaat ttagatcgac ctcgggcgta caaggcaaca
     7261 tcgcaatgcg ccgttggtgc gaccccaacg tcaccggtga aaatatcaaa gcaaaagtaa
     7321 aagaaatcaa cgagcaatac ttacccaaag ggatggaaat taaacccatg gtcgaccgcg
     7381 ctaatttagt cgattacaca ttgcgcacta tcggctcaac actcatcgaa ggcgtcacca
     7441 tcgttacact cattctcatc ttttttatcg gctcggtgcg cacggcgatg gtcgtggtac
     7501 tcacgattcc gttctcactt ctttttgcat ttattctcat gcgcatgacg ggcctctcgg
     7561 caaacctgct gtcgttgggc gctatcgact ttgggattat cgtcgacggt gtggtgatta
     7621 tggctgaaaa cattatgcgc agttaccgcg agaaacgcga aaaccgccat ctgaagttag
     7681 acgaacacac gctcaacagc agtaaagaag tgggcatgga aatcttcttt tcaattttaa
     7741 ttatca
//
LOCUS       DAOMPD010000054         7658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4120_length_7658_cov_2.661449, whole genome shotgun sequence.
ACCESSION   DAOMPD010000054 DAOMPD010000000
VERSION     DAOMPD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4120_length_7658_cov_2.661449"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLY93_03025"
     CDS             complement(<1..282)
                     /locus_tag="PLY93_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679327.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRP68480.1"
                     /translation="MSENVSRKSEITPVRFVTATSLFDGHDASINIIRRCLQRCGAEV
                     IHLGHNRPVHEVVAAALQEDVHAIAMSSYQGGHNEYFKYMIDLLKKYGAS"
     gene            complement(293..1015)
                     /gene="pgsA"
                     /locus_tag="PLY93_03030"
     CDS             complement(293..1015)
                     /gene="pgsA"
                     /locus_tag="PLY93_03030"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRP68481.1"
                     /translation="MRLWLNLPNFLTIMRIVMIPFFIYYLASQHPLDRFIALSLFAFA
                     SVTDFFDGYLARKWKQESPLGRFLDPLADKALVIATLVIFILLDQAIPLWMVIVIILR
                     DMLVTLMRYLAIRKGTEIRTTRLGKAKTMFQMFAMVLIIFILVLRVLPFDISATYAEG
                     QAHGKKNIEIASAMLVKAVHSFQSDEIPKQMRNKYFAESTPYFLLLLTTLITAISGLR
                     YLYTNWRVLLPPYTAILRKSNK"
     gene            1105..1401
                     /locus_tag="PLY93_03035"
     CDS             1105..1401
                     /locus_tag="PLY93_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68482.1"
                     /translation="MDWSQLMVRALEEGSIRELHLYQVPVLKNVENWNQVKEVGKVDF
                     RGKHSDYKGAIVQYGQNYYFLPNSRIDALSVYRKWNFKKAIRVITEAEMKKKAI"
     gene            1491..3473
                     /locus_tag="PLY93_03040"
     CDS             1491..3473
                     /locus_tag="PLY93_03040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68483.1"
                     /translation="MSTTLKRLSRGKCIAIVGLLSAMLLYYFMLMLDGGVDTHIILFS
                     IEFIVGIPIMVYFFFGENWIKNVKKKVAFANVQAFLIASLIGVGFFFQLPAIKNVSIK
                     LTAFSLVIGVVGAAAFTFACNRFTFLLFSLPWLLPVTFFFFFFAGDLGSVSLGGMVVP
                     YVGVLTFLCLKDYTRRVELIRSELSLVEEKEQVVAASQKLQTSLDLVNKLKQQQDGDY
                     YLTSLLLNPLGVNAADEGFNVDVDFFTKQKKAFEFKSHKRNIGGDISIAHTLHFETGP
                     VTVFLNADAMGKSMQGAGGALVLGAVFQSIIERTRLLKNLTPENWLADVFLELQKVFE
                     SFDCSMLISVYFALLENNTGKLYFLNAEHPWGVLYRNGKAIFLQNRSYYRKLGTPGLQ
                     ENIQVDQIQLQSGDIILLGSDGRDDIALPGRHETHRVINEDESIFLRHVEAADGRLEE
                     IYKLISASGEITDDLSLVRVQYNAPKEKVSSPEGALSPQITKAHESHDLQINEPNDKQ
                     NIDIPLTASTLLREAKQKVDTGKTTEAGALLKQAFESASAGPRALRAALNLAMQIRDY
                     ALAIEIAERILPLRSDDAEVLYLAAFAERRLNRLSNAKRYAEKAHQAEGGQHLTTMNL
                     LADIYIELKESANARAMLDEIEPHNETVKKMREALA"
     gene            3475..4338
                     /locus_tag="PLY93_03045"
     CDS             3475..4338
                     /locus_tag="PLY93_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68484.1"
                     /translation="MGRHYVRIHSINDIDTAALTVKTIENRYIDAEGRRYATRFNLRT
                     RKIEIVPIALGREEAVYLKRLANGSLKGTETLTHEAQSERRVHPSPMKLPMWINELGI
                     ERSTHEVDLQNFLNTAERDIQRNGERYRGLIQSLKMSSVLEDLKDANQHDAILEITNT
                     YERDIQSHAGRVQSLVVEFLRFPKPLASYLGQIDRNCKKYVEKLDPEKQKRYSMAYLL
                     AGEMFALHKGCLDLIEMIERFTAEAKPDRLSYERKSAYESAMQSLAFLKESQREEIVT
                     FLGLLTTSDVI"
     gene            complement(4335..5537)
                     /locus_tag="PLY93_03050"
     CDS             complement(4335..5537)
                     /locus_tag="PLY93_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP68485.1"
                     /translation="MFQIANFQQNAFVIVEGKAAPGFFIVRQGKVRLSAEMPIPGEEP
                     SQNLGPGDFFGVVSSMSGHPHIETAQCLAPTSMINVGRDQFGVLIQKNAPIAMKIIRY
                     FSQRLRIIDRAITKLTFHSAVEEDPQMMFNIAQFYSGKQEMKHAAYAYQRYIQFYPNG
                     AFTPQAKMALQNMNAPMSVPTPTGNALARTFADGQMVFIEHEPGNELYIIQGGKVKIT
                     KIVNENEVLLAVLNPGDIFGEMALLENKPRSASAVAFGNVTTLAINKQNFEGMVQAQP
                     QLATKLITLLSERIWLAYRQLANLLISDPVGRMYDTLLTQVQKKKVAIQPKTSYVFDF
                     GSQELLKMLGYPPDKGEEYLITMLSDKNLRLDQGKFTCRDLYELEKQVQFIRKRAATE
                     RQREAAKN"
     gene            5658..>7658
                     /locus_tag="PLY93_03055"
     CDS             5658..>7658
                     /locus_tag="PLY93_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68486.1"
                     /translation="MEDTITQNTSSAIVVQDRSSLTGANSSDMELLAKLDEKLRFIEQ
                     KKINASDPTYYLTLNEVSVANAMQFVASLKKVTDIQNGGKNRVAYLYPILISQHSKPA
                     FQLFLFYPPKNKVGVRDAGPLRRAAISKSEPLLRSLLEAKRSRPTEIDEEDPGAGLIK
                     LDATRGVRFYPDYFSLEYDLRRMLKKNFDRLQLPYIDIPDFFEDIEEFFSRSTDIVER
                     VGEKYHIVIDQLVLDENGNYARTPEVIRHYRLLADGLEKFAFNILLEKAKKAGAGASA
                     FVSSLENYRAQHVLTAEPGRKQNKEKVEALMRIVREYPFDRIQDKENAMLRKSINDVI
                     SWLTLLITKKDRLVERKQRQSADNLLQKIENLIQSNTKQNLDLFQIDLRREILRTVKD
                     EAQAMAMENDFEKRLTTRYGSYKVSQGDGSGYFYFVDQKYISSVLENLSRVMKIDSSA
                     TERFEIANKIFDQLVKRKDPALDQGLSTEERIHLSQMKTYNLNEVTESKRIAMRANRY
                     NIPQGVLVSLILTIFFVAAMFYSTQLFWLIFPFVLISVISFYRLGVSSQNKNRTVQEE
                     TLETVESTTDKAAVMKETQQSIVRQKIASYGSTMVFPFSPQKQSERILTEEEFRAKVK
                     MATPEIKRNVPELASLDGRALEEAAYEALREDAVCIVIPTDIV"
BASE COUNT         2166 a         1665 c         1786 g         2041 t
ORIGIN      
        1 cgatgcgcca tattttttca agagatcgat catgtactta aagtactcgt tgtggccgcc
       61 ttggtaagaa ctcatcgcga tcgcgtgtac gtcttcttgc agagccgcag cgacaacttc
      121 gtgtacgggt cggttgtgac ctaagtgaat gacctccgca ccgcagcgct gcaaacagcg
      181 ccgaataata ttgatcgacg cgtcgtgccc gtcaaaaagt gacgtggctg tcacaaaacg
      241 cacgggtgtt atttcacttt ttctagatac attttcagac ataagggaaa tcctacttat
      301 tcgatttacg taaaatcgcc gtataaggcg gtaatagcac gcgccagttg gtataaagat
      361 aacgcaatcc tgaaattgca gtgatgagag tggtgagtaa aagcaaaaag tacggcgtcg
      421 actcggcgaa atatttgtta cgcatttgtt ttgggatttc gtccgattgg aaagaatgca
      481 ccgctttcac aagcatagcg ctggcaattt caatattttt tttaccgtgt gcttgccctt
      541 cggcgtacgt tgccgaaata tcaaatggta agacgcggag caccagaata aaaatgatga
      601 gcaccatcgc aaacatttgg aacatcgttt ttgctttgcc taaacgcgtc gtgcggattt
      661 ctgtgccttt gcggatggcg aggtagcgca taagggtcac caacatgtcg cgcagaatga
      721 ttacaatcac catccacagt gggattgctt gatctaaaag aatgaatatc acgagtgtgg
      781 caatcaccag cgctttatct gcaagcggat ctaagaagcg tcccaacggc gattcttgtt
      841 tccacttacg cgcaagatag ccgtcgaaaa aatcggtgac tgaggcgaag gcaaaaagcg
      901 agagcgcgat gaaacgatcg agcgggtgct gcgaagcgag ataataaata aaaaatggaa
      961 tcatcacgat ccgcataatc gtgagaaagt ttggcaagtt gagccataga cgcataaggg
     1021 ttatttgaga ttggtagacc ctgcctcaac aaaaatataa gataaaaact tgacttcatg
     1081 ttcgcgtcgt acgaccgtct tacaatggat tggtcacaat tgatggttcg cgccttagag
     1141 gaaggcagca ttcgcgaatt acatttatat caggttcccg ttcttaaaaa cgttgagaac
     1201 tggaatcaag ttaaagaagt cggtaaagtg gatttccgcg gtaagcattc cgactataaa
     1261 ggggcaatcg tgcaatatgg gcaaaactat tattttttgc caaactcacg catcgatgcc
     1321 ctctcggttt accgcaagtg gaatttcaaa aaagcaattc gagttattac cgaggccgaa
     1381 atgaaaaaaa aggccattta acgctcacat aaaaagcgat acaatacgcc acagaagaaa
     1441 ctccatgtac ttatttgcgc gccccgtcac ccaacttgta aaggcagagt ttgtcgacca
     1501 cgttgaagcg actctcgcgc ggcaaatgta tcgccattgt cggattactt tcggcgatgc
     1561 ttctttatta cttcatgttg atgctcgacg gtggggtaga tacgcacatt attttattta
     1621 gcatcgagtt tatcgttggt attcccatta tggtttattt tttttttggc gaaaactgga
     1681 ttaaaaatgt caagaagaaa gtcgcgtttg caaacgtgca ggccttttta atcgcttcgc
     1741 tcatcggtgt aggcttcttt ttccagttgc ctgcaattaa aaatgttagt atcaaattga
     1801 cggcgtttag tctcgtcatt ggcgtggtgg gtgcggcggc gtttaccttt gcttgtaacc
     1861 gatttacttt ccttcttttc tcgttaccgt ggcttttgcc agtgacattc ttctttttct
     1921 tttttgcggg cgatttaggt tctgtaagct tgggcggcat ggtcgtgcct tacgttgggg
     1981 tcttgacttt tttgtgcttg aaagactaca cccgccgcgt cgaactcatt aggtctgagt
     2041 tgagtcttgt cgaagaaaaa gagcaggtgg ttgccgcctc acaaaaatta caaacgagcc
     2101 tcgaccttgt caacaaactc aagcaacaac aagacggcga ttattatctc acttcgcttt
     2161 tattaaaccc gttgggggta aacgccgccg acgaaggatt taacgtcgat gtggattttt
     2221 ttacaaagca aaaaaaagcg tttgagttta aaagtcacaa gcgaaacatc ggcggcgaca
     2281 tatccattgc acacacgctg cactttgaga cagggcctgt gaccgtcttt ttgaatgccg
     2341 atgcgatggg taagtcgatg caaggagccg gtggcgcact cgttttgggc gcggtctttc
     2401 aatcgattat cgaacggaca cgtcttttga aaaatttaac tcctgaaaat tggttggcgg
     2461 atgtttttct tgaactacaa aaagtttttg aaagctttga ttgttcgatg cttatctccg
     2521 tctattttgc tctcttagaa aataataccg gtaaacttta cttcttaaat gcagagcatc
     2581 cgtggggggt tctttaccgc aatggcaaag cgattttttt acagaaccgt agttattacc
     2641 gtaagctggg tacaccgggc ttacaagaaa atatccaagt cgatcaaata caattgcaat
     2701 cgggcgacat catccttctt ggctccgacg ggcgcgacga cattgccttg ccggggcgcc
     2761 acgaaaccca ccgagtcatc aacgaagacg aaagtatttt cttgcgtcat gtcgaagccg
     2821 cagacggtag gctcgaagaa atctataaac ttatttctgc atccggcgaa attaccgacg
     2881 atttgtcgct cgtgcgtgtt cagtataacg ccccaaaaga aaaagtatcc tcccccgaag
     2941 gggcgctctc tcctcaaatt actaaggctc acgaatcgca tgatttacaa atcaatgagc
     3001 caaacgataa acaaaatata gatattccat taacggcttc gaccttgtta cgagaagcaa
     3061 agcaaaaagt cgataccggc aaaacgacag aggcgggcgc tttattaaag caagcctttg
     3121 aatcagcgtc ggcaggacca cgtgccttgc gtgctgcgct gaatttggcg atgcaaatcc
     3181 gagactacgc tttggcaata gaaattgctg aaagaatttt acccctgcgc tccgatgacg
     3241 cagaagtact ctatcttgcc gcctttgccg aaagacggct taaccgcttg tccaatgcaa
     3301 agcgatacgc tgagaaagca caccaagcag aaggcgggca gcatttaacg acgatgaact
     3361 tactcgccga catttacatt gaactcaagg aaagcgcaaa cgcccgcgcc atgttagacg
     3421 aaatcgagcc acacaacgaa accgtaaaga aaatgcgcga ggctttagcg tgatatggga
     3481 cggcactatg tgcgcatcca ctcgattaac gacattgaca cagcggcact aaccgttaaa
     3541 acgattgaaa atcgttacat cgacgctgaa gggcgccgct acgcaacgcg gtttaatcta
     3601 cgtacacgaa aaattgaaat tgtgccgatt gctttagggc gtgaagaagc ggtttactta
     3661 aagcgtttgg caaatggttc tcttaaaggg accgaaactt taacgcatga agcgcaaagt
     3721 gaaaggcgtg tgcatcccag cccaatgaag ttgccgatgt ggattaacga gctcggcatt
     3781 gagcgcagta cacacgaggt agatttgcaa aatttcttaa acactgccga acgcgatatt
     3841 caacgtaatg gcgaacgata tcgaggtctt attcaaagtc ttaaaatgtc atcggtgctt
     3901 gaggacttaa aagacgccaa tcagcacgat gccattttag aaataaccaa cacgtacgag
     3961 cgggacattc aatcacacgc agggcgcgtt caatcactcg ttgtcgaatt tttgcgtttt
     4021 cctaaaccgt tggcaagtta tttggggcaa attgaccgta actgcaagaa gtatgttgaa
     4081 aaattagatc ccgaaaagca gaaacggtat tcgatggcct atctattggc gggtgagatg
     4141 tttgcgctcc ataaaggttg tctcgattta atcgagatga tcgaacgctt taccgccgaa
     4201 gcaaaacccg atcgattaag ttatgagcgt aaaagcgcgt acgagtcggc aatgcaatcg
     4261 ttagcgtttc tcaaagaatc tcagagggaa gaaattgtta cttttttagg gttactcact
     4321 accagcgatg tgatttaatt tttagcagcc tcacgttgac gttccgtcgc ggcacgctta
     4381 cggataaatt gtacttgctt ttcaagttca tataaatcgc ggcaggtgaa tttcccttga
     4441 tccaaacgta agtttttatc cgaaagcatg gtgattaaat attcttcacc tttatctggc
     4501 ggatacccca acattttcaa aagttcttgt gaaccaaaat caaagacata actggtttta
     4561 ggttgaattg ctactttctt cttttgtact tgggttaaaa gagtatcgta catccgccca
     4621 acgggatcgc taataagcag gtttgccagt tgtcgatacg caagccaaat acgctcgctc
     4681 aaaagagtga tgagttttgt cgccagttgc ggctgcgctt gcaccatgcc ttcaaagttt
     4741 tgtttgttaa tggcgagtgt agtgacgtta ccgaatgcta ctgccgaggc ggagcgcggc
     4801 ttgttttcta agagagccat ttcaccaaaa atgtcgcccg ggtttaagac ggcgaggagc
     4861 acttcgttct cgttgacaat cttagtgatt ttgactttac cgccttgaat gatgtaaagc
     4921 tcgttacccg gttcgtgttc gatgaaaacc atttggccgt cggcaaacgt acgcgcaagg
     4981 gcgttgccgg tgggggtagg taccgacatc ggtgcgttca tattttggag cgccatttta
     5041 gcttgtggcg taaacgctcc gttgggataa aattgaatat agcgttggta tgcataagcg
     5101 gcatgcttca tttcctgttt gccgctgtaa aattgcgcga tgttgaacat catttgtggg
     5161 tcttcttcga ccgcgctgtg aaacgttaat ttggtgattg cacggtcgat gatacgcaaa
     5221 cgttgcgaaa aatagcgaat aattttcatc gcaatgggcg catttttttg tataagcacg
     5281 ccaaattgat cgcgcccgac gtttatcatc gaagtgggtg caaggcattg agcggtttca
     5341 atatgaggat gtccgctcat cgacgaaaca acgccaaaaa agtcgccagg ccccaagttt
     5401 tggctcggtt cttcaccggg gataggcatt tcggcgctga ggcgtacttt accttgtctg
     5461 acgataaaga agccgggcgc agccttacct tctacaataa cgaaggcgtt ctgttgaaaa
     5521 ttcgcaatct gaaacatagt cgggctgttt gtctccctta tacttatcgg cagataggct
     5581 gccaatgtga acaaaagaat atgccgtgta tatccgcgct taaatattgg catactgcaa
     5641 agttaaatta gagaagaatg gaagatacta taactcaaaa tacctcaagc gccattgtgg
     5701 tgcaagaccg ttcgtctttg accggcgcca atagttccga catggagctt ctcgccaaat
     5761 tagacgaaaa attgcgtttt atagaacaaa agaagattaa tgcaagcgat ccaacgtact
     5821 atttaaccct caacgaagtt tcggtcgcga acgccatgca attcgttgct tctctcaaaa
     5881 aagtgaccga tatacaaaat ggcggaaaaa atcgagtagc gtatctttat cccattttaa
     5941 tttctcaaca ttcaaaaccc gcatttcagc tctttttgtt ttatccgcca aagaataaag
     6001 taggcgtgcg cgacgctggc ccattgcggc gtgctgcaat atcaaaatcg gagccgcttt
     6061 tacgctctct acttgaggca aaacggtcac gcccaactga gattgacgaa gaagatccgg
     6121 gagcggggct catcaaatta gacgccacac ggggagttcg tttttatccc gattactttt
     6181 ctttagagta cgatttgcga cggatgctta aaaaaaattt tgatcggttg cagttgccct
     6241 atatcgatat tcctgatttt tttgaagata ttgaagagtt tttttcccgt tcaacggaca
     6301 ttgtcgaacg tgtaggagag aaataccaca tcgttatcga tcaactcgtg ctcgatgaaa
     6361 acggtaatta tgcacggaca ccagaggtga tacgccatta ccgcctactc gccgatggtc
     6421 ttgaaaaatt tgcattcaat attctacttg aaaaagcaaa gaaagcagga gcgggggcga
     6481 gtgcctttgt atcgagttta gaaaactacc gtgcgcagca tgtattgaca gcagagccgg
     6541 ggcgcaagca aaacaaagaa aaggtagagg ccctcatgcg catcgtgcgc gagtatccat
     6601 tcgatcgcat tcaagataaa gaaaatgcaa tgttgcgcaa aagtattaac gatgtcattt
     6661 cgtggctaac attattgatt acaaaaaaag acagactcgt tgaaagaaag caacgccaaa
     6721 gcgccgacaa cttacttcaa aaaattgaaa atttaatcca gtccaataca aaacaaaatt
     6781 tggatttatt ccaaatcgat ttacgccgcg aaattttgcg tacagtgaaa gacgaagcgc
     6841 aagcaatggc gatggaaaat gattttgaaa agcgactgac gacgcggtat ggttcgtata
     6901 aggtctcgca aggcgatggc tctggctatt tttactttgt ggatcaaaaa tacataagtt
     6961 cggttcttga aaatttaagc cgcgtcatga aaatcgacag tagtgccacg gagcgttttg
     7021 aaattgcaaa taaaattttc gatcaactcg tgaaacgcaa agatccagcg cttgaccaag
     7081 gcctgtcgac agaagagcgc attcatttat cgcaaatgaa aacgtacaat ctgaacgagg
     7141 taaccgagtc caagcgtatt gcgatgcgcg ccaaccgcta taatataccg cagggggttt
     7201 tagtttctct cattttaacc attttttttg tggcggcaat gttttattca acgcagcttt
     7261 tctggttgat tttccctttc gtgcttattt cagtgatttc tttctaccgt cttggggtta
     7321 gctctcagaa taaaaatcgc acagttcaag aagaaacgct tgaaacagtc gaaagcacaa
     7381 ccgataaagc ggcggttatg aaagaaactc aacaatcgat tgttcgccaa aaaatcgctt
     7441 cttatggttc gacgatggtg ttcccgtttt cgccgcagaa gcaatcggag cgtattttaa
     7501 ccgaagaaga attccgcgcg aaagttaaaa tggccacgcc cgagattaag cgcaacgtac
     7561 ccgaattagc ttctttagat ggccgcgctt tagaagaagc cgcgtacgaa gcgttgcgcg
     7621 aagatgcggt gtgtatcgtc attcccaccg atattgtc
//
LOCUS       DAOMPD010000055         7599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4167_length_7599_cov_2.753579, whole genome shotgun sequence.
ACCESSION   DAOMPD010000055 DAOMPD010000000
VERSION     DAOMPD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4167_length_7599_cov_2.753579"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1179
                     /gene="trpD"
                     /locus_tag="PLY93_03060"
     CDS             157..1179
                     /gene="trpD"
                     /locus_tag="PLY93_03060"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRP68487.1"
                     /translation="MIFYSILEELLAKQDLPNDKITHLISETMQGQLNEVQIAAWLIA
                     MRMKGETPSEIAACAQVMRRMATPIRRGDLPSLFLDTCGTGGDRSHLINISTLVALTL
                     AAQGIPVAKHGNRAVSSASGSADVLEGLGYPLDENPDFIAKRLTQQKFAFFFAPHYHP
                     AMKYAGPVRRSLGVRTIFNILGPLSNPASAPIQLMGVYAPELLIPLAETLKLLGLKAA
                     MVFHSEDGLDEISPIECTHYALLYGDKITTGIYKPPQQNYLTNLDTIRSSTKEKSLQI
                     FKDTIAGRNAAAAYMVSLNAIFGKKLFDMVSKGAHIVDLTPTADDIVEIFDFIQSGAL
                     SDIVQF"
     gene            1423..2643
                     /locus_tag="PLY93_03065"
     CDS             1423..2643
                     /locus_tag="PLY93_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HRP68488.1"
                     /translation="MEWIAAHKEWFVYASMPVTSAFVGWLTNWVAIKMTFYPLKFWGI
                     PPYFGWQGIIPRKAHKMASKSVDLMTTKLIKVEEIFDKVKPAQMAKELAQPMKKVTNE
                     LVDEVGRAANPTVWDLTPGALREEIAKQVNKETGPLLESSIQEIKRNILSVFDLKSLV
                     IKNLTGSNVILLNEMFQRCGKKEFTFIALSGIYFGFPFGIIQMLQWYFYQAPWTVPII
                     GIIVGYYTNWLALKMIFRPLREKKILGLVKYQGLFLKRQKEVSKEYADIVANKILSPH
                     QVLENFIYGRAAEAFFNVIQSNVIRALARVEGIAQPILSFAIGSERYESLKTLIIKRI
                     NEAVPTYANTVEGYLGKAMDLEKTLYERLSNLPPEDFENLLRTAFQEDEWILILVGSA
                     LGFAVGLVQMFFIL"
     gene            complement(2648..4357)
                     /locus_tag="PLY93_03070"
     CDS             complement(2648..4357)
                     /locus_tag="PLY93_03070"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRP68489.1"
                     /translation="MDNYFAKFSSAPLSDADRDKLQSQKDTLVQAGAFSGEPVFGTGG
                     MRAITGLGTNRLNLYNVARLNLALAAYYKRKKETPLCAMAYDSRLTSPEFSRLSYHIL
                     TRAGVRVKIFKRPTPTPFLSFAVRELKADCGVVLTASHNPPQYNGYKAYGADGGQIIS
                     PVDKEIQQLFLDVEYSSLPKGLHVLASTPIPADDLIEEKIYAAYMARLTREAFYQKRE
                     KKKCIWYTPLHGTGGWLFEKAFHDLGYTSFHVLAEQKEPDGNFPTVVSPNPEEPAAFE
                     RILAAAKNAAPDNAPDLLVATDPDADRVGAMVRTQTGAYQFLTGNQIGSLLLESIARR
                     GKASAKSPFICKTIVTTELQRLIAESYGIGTVETLTGFKYIAEQIAKDEDNYLFGGEE
                     SFGYLPISWLRDKDSLSSALALAELAEDTDLSQALDSIYLRHGLFHELLHNINLTSNP
                     NLLPAVMAKLEKASEFIAHVDLGRAVVDVLDLRKGASEPKTQAARALKKTLGTADVIQ
                     FWLKDSSRLTIRPSGTEPKVKIYLSLMGKEKPTASSLAATKEALKNEAEQMLKKFCSA
                     LGV"
     gene            4503..5219
                     /locus_tag="PLY93_03075"
     CDS             4503..5219
                     /locus_tag="PLY93_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRP68490.1"
                     /translation="MRRVLIGIFFITVVSPPFSFAEVAKTNSEKVEAATETKPKAEAT
                     KNNSFAELDKEFQKELAGGPDAQTNNKEQPSILWQFVRTLFTLAILLGIFYALFRLYR
                     FKRNLPAQQFSAVTNLYEYPLGANQRLQIIEIAGRLMILGVSEQSVQLVSEITDKYTI
                     DRIKLDCAEDVKAPRADFLTELSRAIKVNLKERWGQKNTGASSMPPNINDATLDAQRK
                     NSLDRLNKLKSEKNNWRDKP"
     gene            5216..5980
                     /gene="fliP"
                     /locus_tag="PLY93_03080"
     CDS             5216..5980
                     /gene="fliP"
                     /locus_tag="PLY93_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801668.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRP68491.1"
                     /translation="MKVFQRIAIVVFILLSAHALFAQQVPIPRIDFNVTQAKTPQQVA
                     LSMQILLLLTILSLAPAIILMLTSFTKIIIVFDFMKRALSLQNMPPNQVMVSLALFVT
                     FFIMAPTFDEMNRKAITPYLDGQISNTAFFKEAAQPLRKFMIKQIGKDGVKEVALFVR
                     LSKMEKPKSIADVPMQVLIPAFMLTEIKKAFIIGIYLFIPFLIIDMVVASVLMSMGMM
                     MMPPVMISLPFKIILFVLVDGWTLLTYEIVRSYGTS"
     gene            complement(6050..6727)
                     /locus_tag="PLY93_03085"
     CDS             complement(6050..6727)
                     /locus_tag="PLY93_03085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRP68492.1"
                     /translation="MRFIVLRHQDEEGLGTTAEWLQEKGYSQFYVNLTHRQKPPTNSL
                     TAYDALIVMGGNMSANDDSWIMNTSRALTADFVQAGRPVLGICLGAQSIAKVMGATVS
                     PSVPEHGFSYAKPVLTHPFFKSGTLDTNPWDVFQWHRETFTLPNGFQKLFEGDTVSNQ
                     AFTYKNALGLQFHIEMSYEWYAMWQSCHALRGQSTHKLDAPTKNMPFAAMKKNYFALL
                     DCWVKYW"
     gene            complement(6727..>7599)
                     /locus_tag="PLY93_03090"
     CDS             complement(6727..>7599)
                     /locus_tag="PLY93_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRP68493.1"
                     /translation="GSMMLPAAFAASMQQLGQLIDVYLATALMDKVPEAISALTYSHR
                     LIQLPIGIFGVAVATASLSQLSHLHQKDATGQKFSENLAYSIRLNLFFLLPAVAGLMV
                     FSTPIIRVLFERGAFTTRSTDVTATALLCYAPGILAYSLQKLFSVSLYARMNTRTPAI
                     ITGATLLLNLALSLFLMQYFLHGGLALGSALAAWCGVLMYIIILIRTHALRSVRHLFG
                     SVGKLLLLNGIFTAALLTIQKFIFPDHAALQLLIAVPLALLLYIVLAYASHLDEASAF
                     LELLAKLKKKIGRA"
BASE COUNT         2005 a         1782 c         1808 g         2004 t
ORIGIN      
        1 tggagtggtc gaaaaggagg gtaaattttg aggaaaagct ctctttcagg cgggcaggcc
       61 accgcttctt cacctgttta agaccctaat gcattcattc acccatagag cctgaaaaca
      121 attttacggc ctcactcgga aaaatgcact acaccaatga tattctattc aattttagaa
      181 gaattactcg caaagcaaga tttacccaac gacaaaataa cgcaccttat ctccgagacg
      241 atgcaaggcc agttgaacga agtccaaatc gcggcatggc ttattgcaat gcggatgaaa
      301 ggcgagactc cttctgaaat tgccgcttgc gcgcaggtga tgcgtcgcat ggctacaccg
      361 atacgccgtg gagatttacc gtctcttttt ctggatactt gcggtacagg tggtgatcgt
      421 tcgcacctca tcaatatttc aacgctcgtt gcgctcactc tcgctgcgca ggggattcct
      481 gtcgccaaac acggtaaccg cgctgtttca tcggcgtcgg gttcggctga tgttctcgaa
      541 gggctggggt atccgttgga tgaaaatccc gatttcattg ctaagcggct cacccagcaa
      601 aaatttgcat tcttttttgc gcctcattac caccctgcga tgaagtatgc agggcctgtg
      661 cggcgtagtt tgggggtacg gactattttt aatatactcg ggccactttc caatcctgcc
      721 tctgcgccga ttcaacttat gggtgtttat gcgccagagc tattgatccc ccttgcggaa
      781 acactcaaac tcttggggct taaagcagcg atggttttcc atagcgaaga cggtctcgat
      841 gaaatttcac ccattgaatg tacgcactat gcgcttcttt acggcgataa aatcaccacg
      901 ggtatttaca aaccaccgca gcagaattat ctgacgaacc tcgacaccat ccgtagttcg
      961 acaaaagaaa agtcgctaca aatttttaaa gacactatcg ctggtagaaa tgcagcagcg
     1021 gcatatatgg tgagcctcaa cgctattttt ggtaaaaagc ttttcgatat ggtgagtaaa
     1081 ggcgcacata tagtggattt aacgccaact gcagacgaca ttgttgaaat ttttgatttc
     1141 atccaaagcg gcgctttgag cgatattgtg cagttttgaa aagcgcaatc gccatgcgca
     1201 gcgggcccga tgagcgttcg aagcggcaca gtggtgtgtt cagtggatac tccattgaag
     1261 acgcataaat cacatggctg aactaccaag tgtccaaatc cataacccat tgaacgtgtt
     1321 caaataactt gttatatact gtgcagcgca taaattgtaa acacttattt acataatgag
     1381 aaataatgga ctttgccggg tggaaattca acgtggtcga atatggaatg gattgctgca
     1441 cataaagaat ggtttgtgta cgcgagcatg ccggtgacga gcgcgttcgt cggttggctc
     1501 accaactggg tcgcgataaa aatgactttt tatccgctca agttttgggg tatcccgcct
     1561 tacttcggtt ggcaagggat tatccctcgt aaagcgcata aaatggcgtc gaagtcggtc
     1621 gacctcatga cgaccaaact catcaaagtg gaagaaattt ttgataaagt aaaacccgcg
     1681 caaatggcca aggaactcgc gcaaccaatg aaaaaagtca cgaatgaact cgtcgacgaa
     1741 gtcggacgcg ccgccaatcc caccgtgtgg gatctcaccc cgggtgcgct acgcgaagaa
     1801 attgccaagc aagtcaacaa agagacaggg ccactacttg aaagttcaat ccaagaaatt
     1861 aaacgcaaca ttctttctgt gttcgatctc aaaagtcttg tcattaaaaa cttaaccggc
     1921 agcaacgtca ttctattgaa cgaaatgttc caacgctgtg gtaaaaaaga attcacgttc
     1981 atcgctcttt cagggattta tttcggattt ccttttggca tcatccaaat gttgcagtgg
     2041 tatttttacc aagcaccgtg gactgttccc attatcggga ttatcgttgg ctactacacg
     2101 aattggttgg cgctcaaaat gattttccgt cccttacgcg agaagaaaat cttgggactt
     2161 gtgaaatacc aaggactttt tttgaaacgg caaaaagaag tttcaaaaga atacgccgac
     2221 attgtcgcga ataaaattct ttcaccacac caagttcttg aaaattttat ctatggtcgt
     2281 gctgccgagg cgttttttaa tgtcattcaa tcgaacgtca tccgcgcact ggcgcgtgtc
     2341 gaaggaattg ctcaaccgat tttaagtttt gccatcggtt cggaacgtta cgagtcgctc
     2401 aaaacgttga ttatcaaacg catcaacgaa gcggtgccga catacgcaaa cactgtcgaa
     2461 ggctaccttg gcaaggcgat ggatcttgag aaaactctct acgaacgctt gagcaactta
     2521 ccgcccgaag actttgaaaa tctactgcgt acggcattcc aagaagacga gtggattctc
     2581 atcctcgtgg gttcggcgct cggctttgcc gtgggtttag tccaaatgtt ttttatctta
     2641 taaaatttta cacgcctaac gctgagcaaa attttttcaa catttgttcg gcttcgtttt
     2701 tcagcgcctc ttttgtggcg gcaagcgacg atgctgtggg cttttctttc cccatcaggc
     2761 ttaaataaat ttttactttg ggttctgtcc ccgatgggcg gattgtcaac cgcgaagagt
     2821 ctttgagcca aaattggatg acatctgccg tcccaagagt tttctttaga gcacgcgccg
     2881 cttgtgtttt gggctcgctt gcccctttgc gcaaatcgag gacatccacc acagcacgcc
     2941 ccaagtcaac gtgcgcaata aactcggatg ccttttcgag tttagccatc actgcgggta
     3001 gtaagttggg attagaagtt aagttgatgt tgtgcagcag ctcgtggaat aatccatgcc
     3061 gcaaataaat tgagtcgagt gcttgcgata aatcggtgtc ttcggcgagc tcggcaagcg
     3121 ccaacgccga cgagagtgaa tctttatcgc gcagccatga aatcggcaaa taaccaaagc
     3181 tctcttcacc accgaaaaga tagttatctt cgtctttagc aatttgctcg gcgatatact
     3241 taaagccggt gagcgtctcg acggtgccga tgccatagct ctctgcgatg agccgctgca
     3301 actcggtggt cacaatcgtt ttacaaatga acggcgactt cgcgctggct ttccctcggc
     3361 gcgcgatact ctccaaaagc aacgagccga tttggttccc cgtcaggaat tgatatgccc
     3421 ctgtttgtgt gcgcaccatc gcacccacgc ggtcggcgtc ggggtcggtc gcgacgagta
     3481 aatccggggc attgtcgggt gcagcatttt ttgccgcagc aaggatacgc tcgaatgcag
     3541 cgggttcttc ggggttggga gagacgaccg tggggaaatt cccatcgggc tctttttgtt
     3601 ctgcaaggac atgaaaagac gtataaccta aatcgtggaa cgccttttca aaaagccatc
     3661 cacctgtacc gtgcagcggc gtgtaccaaa tgcatttttt cttttcccgc ttttggtaaa
     3721 acgcttcgcg cgttaagcgt gccatatacg ctgcgtatat tttttcttcg atgaggtcat
     3781 cggctggaat cggtgtcgat gccagcacat gtaatccttt cggtaatgaa gagtattcca
     3841 cgtctaagaa aagctgttgg atttctttat ccacgggtga aataatttgt cccccgtcgg
     3901 caccgtatgc tttgtagcca ttgtattgcg gtggattatg cgacgcggtg agcacaacgc
     3961 cacagtcggc tttcaattca cgcacggcaa aagaaagaaa cggtgtcggc gtggggcgtt
     4021 tgaaaatttt aacgcgcaca cccgcgcgcg tcaaaatatg ataactgagt cggctaaatt
     4081 cgggggatgt caaccgcgag tcgtacgcca tggcacaaag tggagtttct tttttgcgtt
     4141 tataatacgc cgctaacgct aaatttagcc gcgctacgtt atagaggttg agtcggtttg
     4201 tacccaagcc ggtgattgcg cgcatccccc ccgtaccaaa aaccggctcg ccagaaaatg
     4261 cgccggcttg aacgagtgta tctttttgcg attggagttt atctctgtcc gcatcgctta
     4321 aaggtgcaga cgaaaatttt gcaaaataat tatccatgat gtgctctata aaagtgttgt
     4381 ttacgattac agcttaagag ttggcgcatc aactaaaaaa caatgatatc tattttagaa
     4441 ttaaaaattt caaattattc ttgccgccct gttttgcatt atgtcacatc ttcattaaga
     4501 ttatgcgccg cgttcttatc ggtatttttt tcataaccgt ggtgagtccc cctttttctt
     4561 tcgcggaagt ggcaaagacg aactctgaaa aggtagaagc agccactgaa acaaaaccca
     4621 aagccgaagc caccaaaaat aactccttcg ccgaactcga caaagagttt caaaaagaac
     4681 tcgcgggtgg tccagacgcc caaacgaata ataaagaaca gccgtcgatt ctttggcagt
     4741 ttgtacgcac cctctttact ctcgctattt tactcggtat tttttatgcc ttgtttcgtc
     4801 tttatcgatt caagcgtaat ttaccagcgc aacaattttc ggcggtaacg aatctttatg
     4861 aatacccgtt gggcgcaaat caacggttgc aaattattga aattgcaggc cgtttgatga
     4921 tactcggtgt ctcggaacaa tcggtgcaac tcgtcagcga aattaccgac aaatatacca
     4981 tcgaccgcat caagcttgat tgcgccgaag atgtgaaggc gccaagagcc gatttcttga
     5041 ccgaactttc acgcgcaatt aaagtcaacc tcaaagaacg gtgggggcaa aaaaatacgg
     5101 gagcctccag catgccgcca aatatcaacg acgcgactct cgacgcacaa cggaaaaatt
     5161 cactcgaccg actcaataaa ttgaaatcag aaaagaacaa ctggcgggat aaaccatgaa
     5221 agtttttcag cgtatcgcca ttgtcgtttt tattcttctt tcggcgcatg cgctttttgc
     5281 gcaacaagtc ccgataccgc gcatcgactt caacgtcacc caagcaaaaa caccgcagca
     5341 agtggcgctg tcgatgcaga tacttttact attaactatt ctctcactcg caccggcgat
     5401 tattctcatg ctcacctctt tcacgaagat tatcatcgtg ttcgatttta tgaagcgtgc
     5461 attgagtctc cagaatatgc ctcccaatca agtgatggtg tcgctcgctc tttttgtgac
     5521 attctttatt atggcgccca cgttcgatga aatgaatcgt aaagcaatca ccccttattt
     5581 agacggccaa atttccaaca ccgctttttt caaagaagca gcgcagccct tacgaaaatt
     5641 tatgattaaa caaatcggta aagacggcgt gaaagaagtc gccctctttg ttcgtttgag
     5701 taaaatggaa aaacctaaat ctattgccga tgttccgatg caagtactca tccccgcatt
     5761 tatgctcacg gaaataaaaa aagcgttcat catcggtatt tatcttttta ttccattttt
     5821 gattatcgat atggtcgtgg cgagcgtttt gatgtcgatg gggatgatga tgatgccgcc
     5881 agtgatgata tcgctaccgt tcaaaattat tctctttgtg ctcgtcgacg ggtggacact
     5941 gttgacatac gagattgtgc gtagctacgg aacttcgtag aataaatagt tcgttgttgg
     6001 actgtcgagc gcatcgactg aacaaagcca ttgcgtaaga gtgttgcgat caccaatact
     6061 tcacccaaca atccaagaga gcgaaataat tttttttcat tgccgcaaac ggcatatttt
     6121 tcgtgggggc atcgagtttg tgcgtgctct gcccccggag tgcatgacaa gattgccaca
     6181 tcgcgtacca ttcgtaagac atttcgatat ggaattgcaa acccaatgca ttcttatacg
     6241 tgaatgcttg gttcgagacc gtatctcctt caaaaagttt ttgaaaaccg ttggggagag
     6301 tgaatgtttc cctatgccac tggaaaacat cccatgggtt agtgtcgagt gtgcctgatt
     6361 tgaaaaaagg gtgcgttaag acaggttttg cataactaaa cccatgttcg gggacgcttg
     6421 gagagactgt cgctcccatg acttttgcaa tcgattgtgc gccaaggcag atgcccaaca
     6481 caggacggcc tgcttgcacg aagtcggcgg tgagcgcgcg tgaggtattc attatccacg
     6541 agtcgtcatt ggccgacata ttgcctccca tgacgatgag cgcgtcgtaa gcggtgagcg
     6601 agtttgttgg cggcttttgc cgatgtgtta agttgacgta aaattgcgag tagccttttt
     6661 cttgaagcca ttcagccgtg gtgcccagtc cttcttcgtc ttggtgccta agtacaataa
     6721 aacgcattat gccctaccta tctttttttt aagttttgcg agaagttcga gaaacgcgct
     6781 tgcctcgtca agatgagagg cgtatgccag tacgatgtat agtagaagcg caaggggtac
     6841 tgcgattaaa agttgtaacg ccgcgtggtc agggaaaata aatttttgta tcgtcaaaag
     6901 tgcagcggtg aaaatcccgt tcaaaagcag gagcttaccg acgctgccga ataaatgccg
     6961 cacgctgcgc agtgcatgag tgcggattaa gataattata tacatcaaca caccgcacca
     7021 tgcggcgagt gcagaaccca acgccaaccc gccgtgtaag aagtattgca tcaaaaaaag
     7081 actcagtgca agattgagta agagcgttgc tccggtgatg attgccggtg tgcgcgtgtt
     7141 catgcgcgcg tatagcgaca cgctaaaaag tttttgtaag ctatatgcta aaatccccgg
     7201 cgcataacac aagagcgcag tggcggtcac gtcggtggag cgggtggtga acgctccccg
     7261 ttcaaaaagg acgcggataa tcggcgtgct aaaaaccatg aggcccgcaa cagcaggaag
     7321 taagaaaaac aaattgaggc gaatagaata cgctaaattc tctgaaaatt tttgacctgt
     7381 ggcatccttt tggtggaggt gcgagagttg tgaaaggctt gcagtcgcta ccgccacgcc
     7441 aaaaattccg atggggagtt ggatgagtcg gtgcgagtaa gtgagcgctg aaatcgcttc
     7501 aggtactttg tccatgagcg cggtggcgag gtacacgtcg atgagctgcc ccaactgttg
     7561 catcgacgcg gcgaacgcag cgggcagcat catcgagcc
//
LOCUS       DAOMPD010000056         7566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4193_length_7566_cov_2.691785, whole genome shotgun sequence.
ACCESSION   DAOMPD010000056 DAOMPD010000000
VERSION     DAOMPD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4193_length_7566_cov_2.691785"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2576)
                     /locus_tag="PLY93_03095"
     CDS             complement(<1..2576)
                     /locus_tag="PLY93_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP68494.1"
                     /translation="MLLKELEILGFKSFADKTKITFSTSVSGVVGPNGCGKSNVLDAM
                     KWVLGEKSVKTMRGEKMEDVIFTGTSTRKAANFAQVSLTFDNRIRLLKIDTDDVKVGR
                     RLYRDGQSQYFINDSRVALKEIEDLFRDTGIGKSSYSFMEQGRMDQILSSKPEDRRLI
                     FEEAAGISRFKAQREEAEKNLQSTQLNLTRVEDIQRSLGRELKSIAEQAKKTTEYNAL
                     MEKFKHHDLKIRYLMVKDANTKIDDLKEKLSRRHAEKEKIQQKLIQMQERLTTLDEDE
                     RRLKEELHKKDVSSQVATEKIAQLSRTLETHESQKNTLQSQLKGLKEQLTRLEKRIKE
                     LKNEAAQQNQFTLKLDLKVSDIEKEIERLDSRLIACADEAKKADSELSRLAKERANSE
                     KKLQQVNARQQETVHELLVSLKNEKARWEKTAQNRDAAFSEIEKLCAEMAANLSALAG
                     GNYPDTAALQNEIQKISDSGKLKSITTNAHLLSEMSRGLHEILFEKGGVHSRKEELDE
                     EIAALEKQIADAIKEEKNLSDTKQRLAEEVAAKRQEREKFIGEINSLNIQKINIKEKE
                     ENLSQQIKNEETNMHFVRAQFTEVDRSVIQLTQDQKNLVREIEKLKSGIEKESQRIQG
                     IEKDIQKVDEKRRELIETMKRESSKTDEVFVAVSELEGKIGTMSGARDLSLQNIYNDY
                     ALSWQELSEKFISGKLVVADEKEKLAELQRAISALGQINQLAIEQHRHVQEMYDHQQA
                     QVDDIMKARADIEKVMADIQQKSEALFRESFEQINNNFANIFGRLFNGGHAQLELLEP
                     DKPLTSGIEIEATPPNKKTKSMRLMSGGEKTMTAIALMFAIYMVRSAPFCVLDEIDAP
                     LD"
     gene            2615..4402
                     /locus_tag="PLY93_03100"
     CDS             2615..4402
                     /locus_tag="PLY93_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRP68495.1"
                     /translation="MVSGYVQGKYAHYHYGYDEGRFSIFAGTEKEKGIPLVSVRDSVR
                     EAERHIGTFRLKPGMLAVVAGVSHLPLLELLYEQMKSPQGQGGWILAFEADTALYMSL
                     KKFRPDLFAEIAVIVPENAGVMAELVEKIDVESFVGYRVFESAAAVGMNPTYYANVIT
                     DFKKKLASRFSDLFTRLEFEERWVFNALSKLPQCVHARPVSALFGLGKGKEALLVSTG
                     PSLRHTLPFLQKNPERYFIACADSAYRVLHRVGIVPHLIFSLDSQAHTTKHFTLLPKG
                     LPNAFPLLVCDAVSNPVVARDWQGELAISFTAQYASASPLPLRGERKVVDEFSSDTRV
                     SELLDGRIVSPGCDFIEDEGYLEGVRGIIPGDLQSGGSVATSIFDLLRLMDFDKIILL
                     GQDLAYTQREIHTPGTHHTDEWLSKTTNRLQSLENINERVLHRRHTKREISITGKPIV
                     TDYILSLYRDWFAHAVEQLPQRVYNATYDGLLLKGAIPYRDEFIQAMQEGPSTAAAND
                     TLLEKYLAAPTLGVNQQKVQSLAVCVESALASNAIDTGTLSFLRYVGRRFQIKAQRTP
                     EMSAPLLKKQTTKQRNFLRKLLTKLYPHA"
     gene            4395..6842
                     /locus_tag="PLY93_03105"
     CDS             4395..6842
                     /locus_tag="PLY93_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRP68496.1"
                     /translation="MPEKKLPLLSRLNARFWPFWLYHVRKLAWPVIAFLLVTSIVGFR
                     YGRKIRIDTNLMHLLPADTASLKALKKMSDESDVKGFLVFSLIAATPQKEGVLLQAAQ
                     KIEQGVLNESFLKAATQKIIYRVPNDFLTRYSLYYADIDDLRKVEARLIATIRKTKQM
                     ENPYFEDLENSSPARFYIGDILNKYRTRYSSTNAFISNDFRQIAILLALNETPENIGF
                     ASRYLTRLKALAADDASLKNFTVSFSGSYATNVEKQKHLQSDINQSTTITLLILISSL
                     IVFFRSLRVVLVIGLPITAALGYTYHAAWFFIGEISIISSFLTSILLGLGIDYGIHLF
                     ARYKNERLKGNGMTQALEATMRNLFRGLSFGMITTASVFLTLSFSRFIAFAEFGKIAF
                     AGIVFFFLSFILFFPALVFVTERFSSGTTEPSHILQKTRKTRGWHILLAVCILGYGIF
                     TLTRLPFEYNFFKLDSSLNASLVKKGNESLRVLENREYMVIYELNNLEEVKAAETNLR
                     ARKTGIEIETHSMLDLIPTGMNEKRAALSRIHSLLKQAELYYLVTGDVKNVQKVEWGL
                     SMTAAPPLTIQKIPEEFREHLVRNGKYYLYIFPKNTKKLKRGALDFAAAYRTVCVQTN
                     VGDACPTGALKTGVSDIYVIDDILQTVMHDIVTELVLIVLVIAAIVLSLTRRAAGVTL
                     ILAPLVAGVLALFAFIGISYQFFPVWLFQFNYINLLAVPILLGVGIDNGIYLYSHTRE
                     LGLSQTNQIMAKTGASIFISNFTTAMGFLSLTISNHKGLASFGFITFAGMVCIFIAYR
                     LLYPALVRFFEKLDTTI"
     gene            6854..7225
                     /locus_tag="PLY93_03110"
     CDS             6854..7225
                     /locus_tag="PLY93_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68497.1"
                     /translation="MGWILKLVLFSGVFNFFLHAQSYDIKTEKNILRSIEEALENGDN
                     PTFTKKCDTLLNPHGINAFAYFLCGKHLLFVPEKDVQKAHAQAKKAYGRLKIAADDFA
                     KTSKQTFYVLDAWQYMGLAAL"
     gene            7226..7408
                     /locus_tag="PLY93_03115"
     CDS             7226..7408
                     /locus_tag="PLY93_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68498.1"
                     /translation="MHDLDRAETYFMRTLERDNRIAAAWYNLGVVYERKGLETEAMRA
                     FDRYLRLQSGESDVDF"
BASE COUNT         1696 a         1828 c         1828 g         2214 t
ORIGIN      
        1 tcgtcgagcg gtgcgtcaat ttcatcgagc acacaaaacg gcgccgagcg caccatataa
       61 attgcgaaca tgagagcgat ggcagtcata gttttttcgc cgcccgacat gagccgcatc
      121 gatttagttt ttttattcgg cggcgtcgct tcgatttcaa taccgctggt gaggggtttg
      181 tcgggctcta aaagttctaa ctgtgcatgg ccaccgttga agagcctgcc aaaaatattc
      241 gcgaagttgt tgttgatttg ctcgaacgat tcgcggaaca acgcctcaga tttttgctga
      301 atgtcggcca tcactttttc aatgtcggcg cgcgctttca tgatgtcgtc cacctgcgct
      361 tgttgatggt cgtacatttc ttggacgtga cgatgctgct cgatggcaag ctggttaatt
      421 tgccccaacg cagaaatcgc gcgttgcaac tcggcgagtt tttctttttc atcggcgacg
      481 actaacttac ccgaaatgaa tttttccgac aactcttgcc aactcaaggc gtagtcgttg
      541 taaatgtttt gcagagataa atcgcgtgcg ccgctcatcg taccaatttt tccttccaat
      601 tcgctcaccg ccacaaagac ctcgtcggtt ttggacgatt cacgcttcat ggtttcgatg
      661 agttcgcgcc gcttttcatc gaccttttga atatcttttt cgatgccttg gatgcgttgg
      721 ctttcctttt caataccgct tttgagtttt tcgatttcac gcactaagtt tttttggtct
      781 tgcgtcaact gaatgaccga acgatcgacc tcggtaaatt gtgcgcggac gaagtgcata
      841 tttgtctctt cgtttttaat ttgttgcgat agattttctt ctttttcttt gatgttaatt
      901 ttttgaatgt tgagcgaatt gatttcaccg ataaattttt cgcgctcttg ccgtttggcc
      961 gctacctctt cggcgaggcg ttgcttggtg tcgcttaaat ttttctcttc ttttatcgca
     1021 tcggcgattt gtttttcgag cgcagcaatt tcttcgtcaa gttcttcttt gcgcgaatgc
     1081 acgccgcctt tttcaaagag gatttcgtgt agtcctcggc tcatttccga caagaggtgt
     1141 gcgttcgtgg tgatactttt caatttgccg ctgtcgctga ttttttggat ttcattttgt
     1201 aacgccgccg tatcgggata gttcccgccg gcgagagcag agagatttgc cgccatttcg
     1261 gcacacagtt tttctatttc gctaaacgcc gcgtcgcggt tttgtgcggt tttttcccag
     1321 cgcgcttttt cgtttttgag cgacactaaa agttcgtgga cggtttcttg ttggcgtgcg
     1381 tttacttgct gcagcttttt ttcggagttt gccctctctt tcgcgagacg agagagctcg
     1441 ctgtctgctt ttttggcttc gtcggcgcac gcgataaggc gtgagtctaa gcgttcgatt
     1501 tctttttcaa tatccgacac cttgaggtcg agtttgaggg taaattggtt ctgttgcgcc
     1561 gcctcgtttt ttagctcttt aattcgtttt tcaaggcgtg tgagttgctc tttaagccct
     1621 ttgagttgac tttggagcgt atttttttga ctttcgtgcg tttctaaagt acgcgaaagt
     1681 tgggcgattt tttcggtcgc cacttggctt gacacatctt ttttgtgcag ctcctctttt
     1741 agccggcgtt catcctcgtc gagcgtggtg agccgctcct gcatttgaat gagcttttgt
     1801 tgaatttttt ctttttcggc gtggcggcga ctcaattttt ctttgaggtc gtcaatcttg
     1861 gtgttggcgt ctttcaccat taaatagcga attttcaaat cgtggtgttt gaatttttcc
     1921 atgagcgcgt tgtactcggt cgtttttttt gcctgctcgg caatcgactt gagctcgcgt
     1981 cccaacgaac gttggatgtc ttccacgcgc gttaaattaa gttgggtgct ttgcaggttt
     2041 ttttcggctt cttcacgttg cgctttgaag cgtgaaatcc ccgccgcttc ttcaaaaatg
     2101 aggcggcggt cctcgggctt gctcgacagg atttggtcca tgcggccttg ttccatgaat
     2161 gagtacgacg acttaccgat gccggtgtcg cggaataaat cttcgatttc tttgagcgca
     2221 acgcggctgt cgttgatgaa gtattggctt tgaccgtcgc ggtaaagacg gcgccccacc
     2281 ttcacatcgt cggtgtcgat tttcaaaaga cggatgcggt tgtcaaaagt gagcgaaacc
     2341 tgtgcaaaat tggcggcttt gcgcgtcgac gtaccggtga aaatcacgtc ttccattttt
     2401 tcaccgcgca tcgttttgac cgatttttcg cccaataccc atttcatcgc gtcgagcacg
     2461 ttggatttac cgcagccatt ggggccaacg acccccgaga ccgatgtcga gaatgtgatt
     2521 ttggttttgt cggcgaacga cttaaaccct aaaatttcga gttctttaag gagcattttg
     2581 cttgaaggtg tgttcttagt tttctcatca ccttatggtc tcgggctacg tacagggtaa
     2641 atacgcccac taccattatg gctatgatga aggccggttt tctatttttg ccggcaccga
     2701 aaaggaaaaa ggcattcctc tggtttcggt gcgcgacagc gtgcgggagg ccgaaaggca
     2761 cataggcacg tttcggctta aaccgggcat gttggccgtc gtggcagggg tgagccactt
     2821 acccctactt gaattactct atgagcagat gaaatccccc cagggccaag gaggctggat
     2881 tctggccttt gaagcagaca ccgcgcttta tatgtctctc aagaaattcc gtccggatct
     2941 ttttgcagaa attgctgtga ttgtccccga gaatgcgggg gtgatggcgg agttggtcga
     3001 aaagatagac gttgaaagtt ttgtgggtta tcgcgttttc gagagcgccg ccgctgtcgg
     3061 tatgaatccc acttattatg caaacgtcat caccgatttc aaaaaaaaac tggcgtcgcg
     3121 cttttcagac cttttcacgc gtctggaatt tgaagagcgg tgggttttta atgcattgag
     3181 taaactgccg caatgcgtac acgcgcggcc agtgtcggcg ctttttggtt tgggtaaagg
     3241 caaagaggcg ttgttggttt ccactggtcc ttcgttgcga cacactttgc catttttaca
     3301 aaaaaatccg gagcggtatt ttatcgcatg cgcagactcc gcgtaccgcg ttttacaccg
     3361 cgtgggtatt gtaccacatt taattttttc actcgatagt caggcgcaca ccacaaaaca
     3421 ctttactctt ctccccaagg gactacccaa cgcgttccct cttttagtct gcgacgcggt
     3481 ttccaatccc gtggtggcac gcgattggca gggtgagttg gccatttcat tcactgcaca
     3541 gtatgcgtcg gcctcgcctc tgccgcttcg cggcgaaagg aaagtggtcg atgaattttc
     3601 gtctgacact cgcgtgagcg agttattaga tggacgtata gtcagtccgg ggtgcgactt
     3661 tatcgaagac gaaggttatc tcgaaggagt gcggggcatt atccctggcg atttacaatc
     3721 gggcggttcg gtggcgacgt cgatattcga tttactgcgc ctcatggatt tcgataaaat
     3781 tattttactg ggccaagact tagcgtacac gcaacgggaa atccacacgc cgggcacgca
     3841 ccacaccgac gagtggcttt ccaaaacgac aaaccggttg caaagtttgg agaacatcaa
     3901 cgagcgcgta ctccaccgcc gccacaccaa gcgcgaaatt tcgattaccg gcaaaccgat
     3961 tgtcaccgat tacattcttt ctttatatag agactggttt gctcacgcgg tggagcagtt
     4021 accgcagcgc gtttacaacg ctacatacga tggccttttg ttgaagggag cgatacccta
     4081 ccgagatgaa tttatccaag cgatgcaaga agggccgagc acagcggccg cgaacgacac
     4141 tcttttagaa aaatacttag cggcaccaac gctcggtgta aatcaacaaa aagtgcaatc
     4201 gttggcggtc tgtgtggagt ctgccttggc aagtaatgcc atcgacacgg gtacgctttc
     4261 gtttttgcgt tacgtcggac gtcgatttca aatcaaagcg caacggacac ccgaaatgag
     4321 cgctccgctt ttgaaaaagc aaacgacaaa gcagagaaat tttttgcgta aattactcac
     4381 caaactttac ccccatgcct gagaagaagt taccactctt gtcgcggctc aacgcgcggt
     4441 tttggccgtt ttggttgtac cacgtccgca aactcgcttg gccggttatt gctttcttgc
     4501 tggtgacgag catcgttggg tttcgatatg gccgcaaaat ccgcatcgac acaaacttga
     4561 tgcatctttt acccgccgac actgcttcgc tcaaggcgct caaaaaaatg agcgatgaat
     4621 ccgacgtgaa aggttttctt gtattttcgc tcatcgcggc aacgccccaa aaagaaggcg
     4681 tgcttttaca agctgcgcaa aaaatagagc aaggggtttt gaacgagtct ttcctcaagg
     4741 cagcaacaca aaaaattatc taccgcgtac caaacgattt tttgacacgc tattcgcttt
     4801 actacgcgga tatcgacgat ttacgcaaag tcgaagcgcg gcttatcgct accatccgaa
     4861 aaaccaaaca gatggagaat ccatactttg aagacttgga aaattcatcc cccgcacggt
     4921 tttatatcgg cgatattttg aataagtacc gtactcgtta cagcagcact aatgcattca
     4981 tctcgaacga ctttcgacaa atcgccattt tgcttgctct caacgaaacg ccggagaaca
     5041 tcggtttcgc ctcaaggtat ttaacacgtc tcaaagcgct cgctgcggac gacgcctcgc
     5101 tcaaaaattt caccgtatcg ttctcgggtt cttacgcaac gaacgtagag aagcaaaaac
     5161 atctacaaag cgacatcaat caatcgacga caatcacact gctgatttta atttcgtcgc
     5221 tcattgtttt tttccgttcg ttgcgcgtgg tgcttgtgat tgggttaccc atcaccgctg
     5281 cgctggggta cacgtaccac gcggcttggt ttttcattgg tgaaattagt atcattagtt
     5341 cgtttttaac gtcgattctt ttggggttgg gcatcgacta cggcatccat ttgttcgcac
     5401 gctacaagaa cgaacgcttg aaaggtaatg gcatgacgca agcactcgaa gcgacgatgc
     5461 gcaacctctt tcgtggtttg agttttggta tgatcaccac ggcatcggtt tttttaacac
     5521 tgagtttttc acggtttatt gcgtttgccg aatttggtaa aattgctttt gcggggattg
     5581 ttttcttttt tctctcgttt attctttttt ttccagcgct cgtctttgtc accgagcggt
     5641 tttcatcggg caccaccgag ccatcgcata tactacaaaa gacgcgcaag acgcgtggat
     5701 ggcacatcct gctggcagtg tgtattttgg gctatggtat ttttactcta acgcgccttc
     5761 catttgaata caattttttt aaactcgatt catcgctcaa cgcgtcgttg gtaaaaaaag
     5821 gcaacgaaag cttgcgcgtg cttgaaaacc gcgagtacat ggttatttac gagttgaata
     5881 atctggaaga agttaaagcc gccgaaacta acttgcgtgc acggaaaaca ggcattgaaa
     5941 ttgaaacgca cagcatgctc gatttaatcc ccacgggcat gaacgaaaaa agagcagcgc
     6001 tttcacgcat ccactcgctg ctcaaacaag ccgagcttta ctatctcgtc actggcgatg
     6061 taaaaaacgt gcaaaaagtt gaatgggggt tgagcatgac tgcggcacca ccgctgacca
     6121 tccaaaaaat tccggaagag ttccgggaac acttggtgcg taacgggaag tactatcttt
     6181 atatttttcc caaaaacaca aaaaaattaa agcgtggtgc gcttgacttt gctgcagcgt
     6241 accgcacggt ttgcgtgcaa actaacgtag gtgatgcatg tcccacaggc gcacttaaaa
     6301 ccggtgtgtc ggatatctac gtcatcgacg atattttgca aacggtgatg cacgacatcg
     6361 tcactgagct tgttttgatt gtgctcgtca tcgccgcgat tgtgctgtcg ctcacgcgcc
     6421 gcgcggcggg ggtgacgctc atcttggcgc cgcttgtcgc gggggtgttg gctctttttg
     6481 cctttatcgg tatttcctac caattttttc cggtctggct tttccaattc aactatatca
     6541 acttgctcgc tgtgcccatc cttttgggtg tgggtatcga taacggtatt tatctttact
     6601 cgcacacgcg cgagttgggc ctttcacaga caaaccaaat tatggcgaaa accggcgcgt
     6661 cgatttttat ctctaacttc accaccgcga tgggcttttt gtcgctcacc atttcaaacc
     6721 acaaaggcct cgcttcgttt ggcttcatca cgtttgcagg catggtgtgc atttttatcg
     6781 cataccgtct cttgtatccg gcgctagtac gcttttttga aaaactcgat acgacaatat
     6841 gaaattttgt ttagtgggat ggatactgaa gctcgtttta ttttctggag tgtttaattt
     6901 ttttctccat gcacaaagct acgacataaa aacagaaaaa aatattttgc gcagcatcga
     6961 agaagcgctt gaaaacgggg acaatcccac ctttacgaaa aaatgcgaca ccttgttgaa
     7021 tccacacggc atcaacgcgt tcgcgtattt tctttgcgga aagcatttac tgtttgtacc
     7081 cgagaaggat gtgcaaaaag cgcatgctca agcgaaaaag gcgtacggtc gtctaaaaat
     7141 tgccgccgat gattttgcaa aaaccagcaa acagactttc tatgttctcg acgcgtggca
     7201 gtacatgggg ctcgccgcgc tctagatgca cgatctcgac cgcgccgaaa cgtactttat
     7261 gcggacgctc gaacgcgaca accgaattgc tgccgcgtgg tacaacttag gcgtggtata
     7321 cgaacggaag gggttggaga ccgaagcgat gcgcgcgttc gaccggtatt tgcgcctcca
     7381 aagcggcgag agcgacgtgg atttttagcg cgctattgaa tctcaaaaat ttcaacgatt
     7441 gtttcacccg aatacgtcga cggcaccgcc acatcggcgc cataaatata ctcggtcgtt
     7501 tcggtattgt tttttatgag tgtcataact cccgtaccca acgtcgcttg atcgactgcc
     7561 gatttc
//
LOCUS       DAOMPD010000057         7555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4201_length_7555_cov_2.821733, whole genome shotgun sequence.
ACCESSION   DAOMPD010000057 DAOMPD010000000
VERSION     DAOMPD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4201_length_7555_cov_2.821733"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLY93_03120"
     CDS             <1..649
                     /locus_tag="PLY93_03120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP68499.1"
                     /translation="SVMARTVLNHDTISSVNRLASLWDVSAGDEWLLPNVRGVAASGS
                     RASVAKKFGKPPESLVSIPGRENYFFVTGLHFEEVESAFFNLRAFIRPVPGRQTSAFG
                     VRSDPFTDKKRFHRGIDLACPIGTKVVASAEGEIIFAGKKGGYGNAVLIRHRNGYITL
                     YGHLSKFAVKKGARVKQGQKIAYSGMTGRATGPHVHFEVRREGKPERPNFHMVKM"
     gene            744..1733
                     /locus_tag="PLY93_03125"
     CDS             744..1733
                     /locus_tag="PLY93_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRP68500.1"
                     /translation="MALVDRLKFNGGPDDIAWKFPDEGITWGAQLIVNESQDAIFFKG
                     GQVHDQFGPGTYTLKSNNIPLLEKFVNKVFGGDTPFAAEVWFISKQIFQGIGWGTKTP
                     IQVEDPKFGIITNLRAFGVYGIRVADVRTFVQQVIGTQGKTTTESIQNLMLSTIVSDI
                     EEMLSQVVHEQNIDIIRINTQRSKIEEAFKPILAKKFERFGIAIEQFEIESLNIPQDD
                     PGFKALQQAKADKAQMDILGDKYAQKRMLDIGEAGAKNEGAGGGMMQAGMGMGMGQMM
                     GQMMGQMNVSGQPQQPAADDPSAKIQKLKGMLDQGLITQAEFDTKKAKILESI"
     gene            1783..2799
                     /locus_tag="PLY93_03130"
     CDS             1783..2799
                     /locus_tag="PLY93_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRP68501.1"
                     /translation="MILIDRFAGTVRGKEKLSLPHLVIAQTNTQIAAILRGDLKILKQ
                     LSAYVVASGGKRIRPLFLYYLGKAHELDGGELIRLGALLEIIHAASLLHDDVVDGADE
                     RRGKPTAGKLYGNKKVVLGGDHLLSSGLKYLNSLKNPDYMTVFTDAICALTEAELIQM
                     QHHGNFKTTLETHARVVDGKTAVLFRAAGALVAILRKDYKQDFYHSETAALGALFGRY
                     FQHRDDFLDYFDAARLKKKGLQDFTNGIATRPFIFLRERANKEDLKLLQREWNTSSAQ
                     KNKEPNKIILQLMQKYSIEEKCQELLLVDEKRIMDALSALPEKKARGIIRTEFEKILA
                     VRAK"
     gene            2841..3362
                     /locus_tag="PLY93_03135"
     CDS             2841..3362
                     /locus_tag="PLY93_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP68502.1"
                     /translation="MKKLIVITLLAAATALWAKSAEENIADLKSADDAVAIKAAQELG
                     ADGAKAAIEPLGDVVKSNRSTGVRIAATSALGRIAEKGRTTTILKESIEADQDNQVVY
                     TSLLALMNIADVTNPDLVKAIEFCETNKKSDPFISDIVERLRKKFPNDAAKAEKKPAE
                     EKPAEQPAEKPAE"
     gene            3500..4402
                     /locus_tag="PLY93_03140"
     CDS             3500..4402
                     /locus_tag="PLY93_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68503.1"
                     /translation="MPKDVHFTVQQGRVSFLCKEDTFFLRGTATRTLAWQRTDQIFAT
                     LAKELNATPIETWTTGKLYKVKNTKNTASDKMSGKELICAYVVRDTYALDVCANYKEN
                     ETGNSSEKMIRRLISEFQTVAFEKMPLLQSAPLFSTRRVFTEENDTREPALLALTMPA
                     GYVEVTRRENRSLFTDAAGLAEVEIFYEQSELALDSALAHKAYKKFITSFLTKQGIWH
                     AVETKEDTTYKGAVCLLFTDNDKRSSNYCYIVAALNQNHKKKYFRVVFVAAFLRDVVD
                     ETVLLAEQKKLLQNWMATLVKHSG"
     assembly_gap    4432..4441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4442..5143
                     /locus_tag="PLY93_03145"
     CDS             <4442..5143
                     /locus_tag="PLY93_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRP68504.1"
                     /translation="ERYSRQILLRGIAVQEKIKAACVLVVGAGGLGTTMLPLLAASGV
                     GKIILFDHDRVERSNLGRQWLFRESDIGAYKATTVKNFLTALNPYTTVMARTEKFTQQ
                     HADVLRESTIALEGSDSLSTKFLINDLAKRHQRPALIAALGAQQGHAMFVGEGACYRC
                     LFDEVDSGDLPTCASEGILSTFPSVVGSAVAHTALEFLLTGDGASKFWIFEKNTCRKI
                     LIPQNKDCQQHIYDT"
     gene            5133..>5843
                     /locus_tag="PLY93_03150"
     CDS             5133..>5843
                     /locus_tag="PLY93_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP68505.1"
                     /translation="MIRRKKTYALALSGGAALGAYQAGALKAIYEAGLNIRYIAGSSV
                     GALNGYLAATGEIKELVEFWQGIDQKKLITMNSFTKLFFSAIPSLLTDEIQRELVTKY
                     ARVEKLKDTGREFVCTAININTGKLHFFEGHNAASDEELQLMILASAAVPGVFPPVEM
                     NKHLYMDGGLVRNTPIEAFYRKNVHAVIGVSMEPETYPDTPLQNSGQIALRTVSIFFR
                     SQANDAVERTRLSRKRKAK"
     assembly_gap    5844..5853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5985..6965
                     /gene="add"
                     /locus_tag="PLY93_03155"
     CDS             5985..6965
                     /gene="add"
                     /locus_tag="PLY93_03155"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRP68506.1"
                     /translation="MIDLHHHFDGAFDINALYQEAKRRNLPQGNLSSEEFATHCQVKP
                     ESRSLSAFLAVFDFFYGIAQDIDFLHKEALALPQRMAEQGVLYLETRFAPHLFTATHY
                     SAEAVVEAAVTGLEKNENKNVRLILCTMRNAPVETLQEVIELYTKFNARGVCGIDLAG
                     DESRYACREYAPFFARAHEQKIPITIHAGEAAGAHSVYDALDLFHARRLGHGIRAIDD
                     AKLIERLCAERIGVEVCLTSNLQTGNAASYAEHPFMRLYRSGVCVTVNTDDPSVSGID
                     LAHEWSIAEREFHLTDADKKQLLFNSIEVAFCDNTLKEKLRQEVTARLAK"
     gene            6975..7523
                     /locus_tag="PLY93_03160"
     CDS             6975..7523
                     /locus_tag="PLY93_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRP68507.1"
                     /translation="MRHKTLWFIAILPPDDVTQSVRTVQQEIADFYGPSRALKVPVHI
                     TVASPFRYGEDENATFTPLLESFFTNENTFSLELRNFGSFREDVIFIEVSPSLPLLEM
                     HARLTTLLKENEIVVHDFRHGGFTPHATVANRDVDAHTHRTLWREFNTRKFYAKFIVA
                     DVALLRHDGNVWHVHQKFALKK"
BASE COUNT         2131 a         1806 c         1798 g         1800 t
ORIGIN      
        1 ttcagtcatg gcgcgcacgg tgcttaatca cgacactatt tcaagcgtga atcgtctcgc
       61 atcgctgtgg gatgtcagcg cgggcgatga atggctactg ccgaatgtgc gcggggttgc
      121 tgcgtcgggt agccgcgcaa gcgttgcaaa gaaatttgga aaaccccccg agtcgttggt
      181 gtctatcccc gggcgcgaaa attatttttt tgttacgggt ttgcattttg aagaagttga
      241 atcggcgttt tttaatttgc gcgcgttcat tcgccctgtg cctggtcgac aaacgtccgc
      301 gtttggcgtg cgtagcgatc ctttcaccga taaaaaacgt ttccatcgag gcatcgattt
      361 agcgtgcccc atcggtacaa aagttgtcgc ctcggcagaa ggcgagatca tctttgccgg
      421 gaaaaaaggc ggttatggca acgcggtgtt gatccgccat cgcaacggtt acatcactct
      481 ctatggtcac ttgagtaagt tcgcggtaaa aaaaggggca cgcgtgaagc aagggcaaaa
      541 aattgcatat tcgggcatga ctggccgcgc caccggcccg cacgttcatt ttgaagtgcg
      601 ccgcgaagga aagccagagc gtcccaattt tcacatggtc aagatgtagc tccgttcttt
      661 agctattcaa aaatgaaaaa aataatggca ggtacactca aaatatgtta tatggtaggg
      721 aaactcttgc ggaggacaat aatatggcat tagtagacag acttaaattc aacggaggcc
      781 ccgacgatat cgcgtggaag tttcccgacg aaggcattac atggggtgca cagctcattg
      841 taaacgaatc acaagacgca atttttttca aaggcggtca agtacacgac caatttggtc
      901 caggtacata cacccttaaa tcgaacaaca tccctctctt agaaaaattt gtaaataaag
      961 tttttggcgg ggatacccca ttcgctgccg aggtgtggtt catttcaaaa caaatttttc
     1021 aaggcatcgg ttggggcact aaaactccga ttcaagtgga agatcccaaa tttggtatta
     1081 tcacaaactt acgtgcattc ggtgtctatg gtattcgtgt cgccgatgta cgcacattcg
     1141 tacagcaagt gattggtaca cagggtaaaa caactaccga gagtatccaa aacctcatgc
     1201 tttcgacaat cgtctctgac atcgaagaaa tgctttcaca agtggtacac gagcaaaaca
     1261 tcgatatcat ccgtatcaac acgcagcgct ctaaaattga agaagcattt aaaccaattc
     1321 tggcaaaaaa gtttgagcgt ttcggtattg cgattgagca atttgaaatc gaatctctca
     1381 atattccaca agacgatcca ggcttcaaag cattgcagca agcaaaagcc gacaaagcgc
     1441 aaatggatat tttgggcgac aagtacgcac aaaaacggat gctcgacatc ggcgaagcgg
     1501 gtgcaaaaaa cgaaggcgct ggcggcggca tgatgcaagc gggcatgggc atgggcatgg
     1561 gacaaatgat ggggcagatg atgggacaaa tgaatgtctc cggccagcca caacagcccg
     1621 cagccgacga tcccagcgca aaaattcaaa agctgaaagg gatgctcgat caagggttaa
     1681 tcacacaagc agagtttgat actaaaaagg cgaaaatcct cgagagtata taaggaaaat
     1741 tgtgctttac gactttaatg ttacggcaca gattcaaaga cgttgattct tattgatcga
     1801 tttgcgggca cggtgcgcgg taaagaaaag ctttctctac cgcaccttgt tattgcgcag
     1861 actaatacgc aaattgccgc cattttgcgc ggcgacttaa aaatcctcaa acaactttcg
     1921 gcgtatgtcg ttgcctcggg gggtaaaaga atccgccctc tttttctcta ctatttaggt
     1981 aaggcgcatg agttagacgg tggcgagctc attcgcttgg gcgcgctatt ggaaattatc
     2041 cacgcggcga gtcttttgca cgacgatgtc gtcgatggcg ccgatgagcg gcgcggcaag
     2101 cccaccgctg gaaaacttta tgggaataaa aaagtcgtct tgggtggcga ccaccttctt
     2161 tcctcggggc ttaaatacct aaactcgctc aaaaatcccg actacatgac tgtcttcacc
     2221 gacgccatct gtgcacttac agaagccgag ttgatacaaa tgcaacacca cggtaatttc
     2281 aaaacgactc tcgaaacgca cgcgcgcgtc gtcgacggca aaacggcggt cttgtttcgc
     2341 gcagcgggag cactggtggc aattttgcgc aaagactata agcaggattt ctaccattct
     2401 gaaacggcgg cactgggcgc attgtttggc cgttactttc aacaccgcga cgatttctta
     2461 gattatttcg atgcggcacg actcaagaaa aaagggttgc aagattttac caatggtatc
     2521 gccacccgtc cgtttatttt tttacgcgag cgcgccaata aagaagattt aaagctttta
     2581 cagcgcgagt ggaatacaag cagcgcacaa aaaaataaag agcctaacaa gataatctta
     2641 cagcttatgc aaaagtacag catcgaagaa aaatgccaag aactactgct tgtagacgaa
     2701 aaacgtatta tggacgcttt aagtgcatta ccagaaaaaa aggcgcgcgg cattattcgg
     2761 acggagtttg aaaaaattct tgccgtgcgc gctaagtaaa tcttaattac tgtataatca
     2821 aagggagcaa taaacgctat atgaaaaaac ttattgtaat tacattgttg gcagcagcca
     2881 ctgcattgtg ggccaaaagc gccgaagaga atatcgccga cctcaagtcg gctgacgatg
     2941 ccgtcgcgat taaagcggcg caagaattag gcgccgatgg cgctaaagca gcgattgaac
     3001 cattaggcga tgtcgtcaaa agtaatcgct caacgggtgt gcgtattgcc gcaacgtcgg
     3061 cactgggacg cattgcagaa aaaggccgca caacgaccat cctgaaagaa tcgattgaag
     3121 ccgatcaaga caaccaagtt gtgtacactt cactcttagc gctcatgaac atcgccgatg
     3181 tcacaaaccc cgacttagta aaagctatcg aattttgtga aacaaataaa aaatccgatc
     3241 cttttattag cgatatcgtc gaacgcttac gcaaaaaatt ccccaatgat gcggcaaaag
     3301 cagaaaagaa acccgcagaa gaaaaaccag cagaacagcc tgctgaaaaa ccggcagaat
     3361 aaactcaata atttcttttg tgagaattgc agccaaactc aatcgcgggt acattttact
     3421 cgcattttgg tttggctcct cccttagcct ttttgctgcc ggcgcaaaaa cgcaatgcga
     3481 tttttctctc aaaactccag tgcctaaaga cgttcatttc acggtgcagc agggccgtgt
     3541 ttctttcttg tgtaaagaag ataccttttt tcttcgcggt accgccactc gcacattggc
     3601 gtggcaacgc accgatcaaa tctttgcaac gctcgcaaaa gaattaaatg caacccccat
     3661 tgaaacttgg actaccggta aattgtataa agttaaaaat acaaagaata ccgcatcgga
     3721 taaaatgagc ggaaaagaac ttatttgcgc ttatgtggta cgcgatacct acgcactcga
     3781 tgtgtgcgcc aactataaag aaaacgagac tggcaatagc tctgaaaaaa tgatccgtag
     3841 gttaatcagc gagttccaaa ccgttgcatt cgagaaaatg ccgcttctgc aatcggcgcc
     3901 gctttttagc acccgccgcg tcttcaccga ggagaacgac acccgcgaac ccgcgctttt
     3961 agctctcacc atgcccgcag gttatgttga agtcacacgc cgtgaaaacc gttcgctatt
     4021 caccgacgcc gccggtttag ctgaagttga gattttttac gagcagtcag aactcgcgct
     4081 cgactctgca ctggcgcaca aagcctataa aaaatttatc acgtcatttc tcaccaaaca
     4141 agggatatgg catgccgtag agactaaaga agatacaaca tataaaggcg cagtatgtct
     4201 tcttttcacc gacaacgata aaaggtcgag caactactgc tacattgtcg cagcgctcaa
     4261 tcaaaaccat aagaaaaaat atttccgtgt cgtattcgtt gcggcatttt tacgcgacgt
     4321 ggtcgacgaa acggtactgt tagccgagca aaaaaaactt cttcaaaatt ggatggcgac
     4381 gctcgtcaag cattcgggat aaattggcta ctttctcaaa agaagaaatt gnnnnnnnnn
     4441 ngagcggtat agtcgacaaa ttcttttgcg cggtatcgcc gtgcaagaga aaatcaaagc
     4501 cgcgtgcgta ctcgtcgtgg gtgcgggcgg tttaggcacg acgatgctgc cgcttttggc
     4561 ggccagcggc gtgggcaaaa ttattctttt cgatcacgat cgcgtcgagc gcagcaattt
     4621 gggcaggcag tggctttttc gcgaaagcga catcggcgca tataaagcta ccacagtaaa
     4681 aaatttttta accgctctca atccctacac cacagtgatg gctcgcacgg aaaaatttac
     4741 acaacaacac gcggacgtac ttcgagagtc aaccatagct ttagaaggga gcgactctct
     4801 ttcaacaaaa ttcctcatca acgatttagc gaaaagacac caaagacctg cgctcatcgc
     4861 tgcgttgggc gcacagcaag ggcatgccat gttcgtgggc gagggcgcgt gctaccgctg
     4921 cctttttgac gaagtggaca gcggcgattt gccgacgtgt gcaagcgaag gaattttgag
     4981 cactttcccg tcggtggtgg gttcggccgt ggcgcacacc gcgcttgagt ttctattaac
     5041 gggtgacggc gcatcgaagt tttggatttt tgaaaaaaat acttgccgca agattttaat
     5101 cccacaaaat aaagattgcc aacaacacat ttatgatacg tagaaaaaaa acttacgctc
     5161 tagcgctttc gggaggggcc gcgttgggcg cgtaccaagc gggcgcactc aaggccattt
     5221 atgaagccgg gttaaatatc cgctacatcg ccggcagctc ggtgggtgcg ctcaacggtt
     5281 atttagccgc cacgggagaa attaaagagc tcgttgagtt ttggcaaggc atcgatcaaa
     5341 aaaaactcat cacaatgaac tcatttacta aattattttt ttcagcgata ccctccttat
     5401 taaccgatga aattcaacgc gagctcgtga cgaaatatgc acgcgttgaa aaactaaaag
     5461 atacggggcg tgaatttgtt tgtaccgcga tcaacatcaa caccggcaag ctccattttt
     5521 ttgaaggcca taacgccgca agcgacgaag aattacaact gatgatttta gcgtcggcgg
     5581 cggtgccagg ggtttttcca ccggtagaaa tgaataagca cctttacatg gacggcggct
     5641 tggtgcgtaa cacacccatc gaagcatttt acaggaaaaa tgtgcacgcc gtcatcggcg
     5701 ttagcatgga acccgaaaca taccctgaca cgccgctaca aaatagcggc cagattgcgc
     5761 tacgcacagt gagtattttt tttcgctcgc aggcgaatga tgccgtcgag cgcacacgtt
     5821 tgtcgcgcaa acgcaaagcg aaannnnnnn nnnaagattt tattattgcg cccgcgccta
     5881 ccgttggaga tgcaacaata cgaattcgac cctgtgaaaa ttgccgcgct gattgaacag
     5941 ggctaccgag aagcgcagca gtttattaaa acacataaat taacatgatt gacttacacc
     6001 accattttga tggggcattc gatataaacg ccctctatca agaagccaag cggcgcaatt
     6061 tgccgcaagg taatctctcg tctgaagaat ttgcaacgca ctgccaagtc aaaccagaaa
     6121 gccgttcgct ctccgccttt ttggcggtat tcgatttttt ctacggcatt gcgcaagaca
     6181 tcgatttttt gcacaaggaa gcgttggcgc tcccccaacg gatggcggag caaggcgtgc
     6241 tttatttaga aacacgtttc gcgccgcacc tttttaccgc gacgcattat tcggctgaag
     6301 cggttgttga agccgctgtt acgggattag aaaaaaatga aaataaaaat gtgcgcctta
     6361 ttttatgcac catgcgcaac gcaccggtag aaaccttgca agaagttatt gaactctaca
     6421 caaaatttaa cgcgcgcggt gtgtgtggca tcgacttggc aggcgacgaa tcacgctacg
     6481 cttgtcgcga gtatgcccct ttctttgcgc gtgcacacga acaaaaaatt cccatcacca
     6541 tccatgcggg cgaagcggca ggcgcgcact cggtgtacga cgcgttggat cttttccacg
     6601 cgcggcggtt gggccatggc atccgcgcca tcgacgacgc taagttgatc gaacgccttt
     6661 gtgccgagcg cattggtgtc gaggtttgtc tcacaagtaa tttacaaaca ggcaatgccg
     6721 cttcttacgc cgagcaccct ttcatgcgac tataccgtag cggtgtgtgc gttaccgtca
     6781 acaccgacga ccccagtgtg tcaggcatcg accttgcaca cgagtggagc atcgctgaga
     6841 gagaatttca cttaaccgat gcggataaaa aacaacttct ttttaattct atcgaagtgg
     6901 cgttttgcga caatacactt aaagaaaaat tgcgtcaaga agtgacggca cgcctcgcaa
     6961 aataatgttc gtccatgcgc cacaagacgc tgtggtttat cgccatttta ccccccgatg
     7021 acgtaacgca aagcgtacgt acagtacaac aggaaatcgc cgatttttat ggcccttctc
     7081 gcgcgcttaa agttccggtg cacatcaccg ttgcgtcgcc tttccgctat ggcgaagatg
     7141 aaaatgctac gtttactcca ctacttgaaa gtttttttac taacgaaaat acgttttcgt
     7201 tggagttgcg taatttcggt tcgtttcgag aagacgtaat ttttatcgaa gttagtccct
     7261 cgttgccgct tttggaaatg cacgcgcgtc tcactacgct tttaaaagaa aatgaaattg
     7321 tcgtccatga ttttaggcat gggggattta ctccgcacgc cacggtggcg aaccgcgatg
     7381 tcgatgcaca cacacaccgc actttgtggc gtgagtttaa cacacgaaaa ttttacgcaa
     7441 agtttatcgt cgccgatgtt gcgcttttgc ggcacgacgg caacgtgtgg catgtacacc
     7501 aaaaatttgc cctaaaaaaa tagcgctatg ccgatggaag atttacaaaa ccgat
//
LOCUS       DAOMPD010000058         7510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4226_length_7510_cov_2.809926, whole genome shotgun sequence.
ACCESSION   DAOMPD010000058 DAOMPD010000000
VERSION     DAOMPD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_7510_cov_2.809926"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLY93_03165"
     CDS             <1..344
                     /locus_tag="PLY93_03165"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HRP68508.1"
                     /translation="ELLDDLVPKDLKANTSSDIFSRTPAREKIITHIGNNPERQKMFE
                     AARQFENFFLEKMFREMRKNMGKDDILHGGAAENIFQDMLLTERVNTLTQSATLGLAE
                     QIYTQMQRTQS"
     gene            341..1108
                     /locus_tag="PLY93_03170"
     CDS             341..1108
                     /locus_tag="PLY93_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRP68509.1"
                     /translation="MRKSHFRFFIYLFIFSIVSCLYAQEPSSTPETKPPTTIHTSPSI
                     FKSPALKRAEKFYFSKKYETAAALLRKIIDDEPENGRANSLLGDVYYLQGHYKKAVSH
                     LLRATELNESKANDFFRLGQTYIKLNQSKEALSAFQKAYENDDKMKSALFMSGFVYLS
                     LERNKKKTIEFWQRFVDEATHDPQREKVATVLKYLSDPNFVLPNKNSPISLEEALLIG
                     GKAGPAETKTGEDKGAGHENAKEKNDSKELLDDDSLN"
     gene            1119..1484
                     /locus_tag="PLY93_03175"
     CDS             1119..1484
                     /locus_tag="PLY93_03175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP68510.1"
                     /translation="MPQANDRRSQHRVTAGAYIEYRVTIDSTQVKDAIMSDISEGGLM
                     LLVEEADLGRKFNIDDALSGHIIHDRTKFNFPYTGKVAWLQPREMRTRKFVAMGIRFD
                     DGIELPAQIYAILMADHDF"
     gene            1528..2400
                     /locus_tag="PLY93_03180"
     CDS             1528..2400
                     /locus_tag="PLY93_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP68511.1"
                     /translation="MLKICGGWLTVLSLIVILTVFLQHLRKGSAKEVLMGQRASRAAL
                     KNLKEKSFGTKFNEFVDDLPNLLHWKTLRGTKLIPQITDGLQNTALVSGLSLLFALGI
                     GFIFLITAELHPKTALFLDRFANSINTVPIFLLGIFFIWLFAFALRLLPAGGDQNLRD
                     FILPALSIATKFGARLFLLLKEYMNELERKVFILRARAFGLSRKRIFTHKLLNCLMPF
                     IIFWLIETASLFSGAVIVETLFSIHGLGSLLLFALLQYDIRLIFVNLAVIATIVYATS
                     ILQAQLSRFQESAQ"
     gene            2397..3164
                     /locus_tag="PLY93_03185"
     CDS             2397..3164
                     /locus_tag="PLY93_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP68512.1"
                     /translation="MKRILRLYYAPSIVGCLLLFSLFLPKNEIQLENAFLSASWNAPL
                     GTTPLGESILTQVLVGAGQTLTLSFAALSVCLTFSLTIGGILFQLPRPLQKAYTFFID
                     AWLSIPSIFIALSIGYFLPQSFFSVFVALVLSEFAALQKFFVQRLQETQSSDFLTAAR
                     VMGASKAHLAAKHILPRLVRDALYLFIITLPSIVLSLASLEFLGVQTGSSTLSLGMQI
                     ALYKDYIFLYPTLSLAPVLCILFILFLLNETAKVSRA"
     gene            complement(3215..4279)
                     /locus_tag="PLY93_03190"
     CDS             complement(3215..4279)
                     /locus_tag="PLY93_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68513.1"
                     /translation="MKRSNSKYDGLVCTFDLDKTYLDTRFESFRKLVRIPFEKAEEKK
                     NIPGVAAVVREIRRGLDSTKPPVPIYFISGSPEGLHKVVAEKLKLDGVAFDGILFKNW
                     RAAVRKFQFKKIIDKIGFKLAALIYARGIFPVRAEEILFGDDSEYDAAIYSLYDDIAA
                     GKLEYYEVLTILKKWQVAQDEMKLIADNLKRLADSGYKPREAVRQIFIHLEQKTSASL
                     HTTLSEKVIPTQNYFQTAVILYKSDVITRAGLFRVISDMIRNYNFGANEFTVSTEDLL
                     RRRLIDTGEARKLLKTIHKGTAFALPRRIIADFRSNLLRTLDSMSRMPETIMPPRRRD
                     SDKNISLVERYLQFEPKRNV"
     gene            complement(4302..>5034)
                     /locus_tag="PLY93_03195"
     CDS             complement(4302..>5034)
                     /locus_tag="PLY93_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRP68514.1"
                     /translation="WSLITWYFGIPSSSSHTLIGGFAGAAVAHAGFGVVKMAKIWPIL
                     AFIVLAPFLGMIMSYMNSIILLHICKNTHPMRAERWFRRLQLLSSAAFSLGHGGNDAQ
                     KVMGIIGAAMLVSGKIQVLTELPQWVVISCYSAIGAGTLMGGWRIVKTMGTKITKLTA
                     FEGVASETAGAVTLFTTGEFGIPVSTTHTITGSIIGVGLAKRISSVRWGITVKLFWAW
                     IITIPISGLFALFFYHLIHLFVPDA"
     assembly_gap    5035..5044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5045..5302)
                     /locus_tag="PLY93_03200"
     CDS             complement(<5045..5302)
                     /locus_tag="PLY93_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRP68515.1"
                     /translation="MELFVVVIILALLFDFINGFHDAANSIATIVSTRVLSPLQAVIW
                     AAFFNFLAYYIFEHHVADTVAKTVDTSAITLFVIMAGLLAAI"
     gene            complement(5304..5951)
                     /locus_tag="PLY93_03205"
     CDS             complement(5304..5951)
                     /locus_tag="PLY93_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRP68516.1"
                     /translation="MSLTSFFRFLSPKDRTFGPLFKQDTDNLIQLADALTQLMAEKNA
                     KQREKLVKEIDRLENVGDDITHSIYVALEESFITPFDREDVHALASSMDDIADNIQGA
                     AARTRLYHIANFPGPSVEIAKVLKKQIEILAQATPLLMNLKDRHKITELLVQVHSLEN
                     QADHIFNAAIKDLFLKQKNAINLIKMKEFFAVLETATDSCEDVADVMESIVIKYS"
     gene            6039..>6315
                     /locus_tag="PLY93_03210"
     CDS             6039..>6315
                     /locus_tag="PLY93_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HRP68517.1"
                     /translation="MRTAQIERKTNETAIRLALNLDGTAKMTGENPIAFFEHMLGHIV
                     KYSMFDLDLHLKGDLAIDCHHSVEDTGIILGDALRQALGNKGGIFRYG"
     assembly_gap    6316..6325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6326..6663
                     /locus_tag="PLY93_03215"
     CDS             <6326..6663
                     /locus_tag="PLY93_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HRP68518.1"
                     /translation="GIRRFGHAYIPMDETLTTVTVDLSGRPYFVYRGEPLKPMGKFGI
                     YDSELTLEFLHKLSIHAAMNLHVQVHYGENRHHIHESIFKALGFALRQAVAIDARRAH
                     EIPSTKGTL"
     gene            6670..>6895
                     /gene="hisH"
                     /locus_tag="PLY93_03220"
     CDS             6670..>6895
                     /gene="hisH"
                     /locus_tag="PLY93_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRP68519.1"
                     /translation="MHKSQARIALIDFGMGNIRSLQKAFEFLGHAAVVTGDYKEAQKA
                     DVLLLPGDGAFARAMHGLKEQHLDEVLYEAH"
     assembly_gap    6896..6905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6906..7303
                     /gene="hisH"
                     /locus_tag="PLY93_03225"
     CDS             <6906..7303
                     /gene="hisH"
                     /locus_tag="PLY93_03225"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRP68520.1"
                     /translation="QKIIFGVCIGFQLFFESSTEFGGAKGLGLVKGAITRLEKNADAR
                     WIPHVGWSTTVFEEDSLLGRGIYPEAMFYYVHSYAHRAQHEYAAATCDYGQIFTSVVE
                     HENLFAVQFHPEKSHRAGLKLIQNFLEVL"
     gene            7300..>7510
                     /locus_tag="PLY93_03230"
     CDS             7300..>7510
                     /locus_tag="PLY93_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRP68521.1"
                     /translation="MKLIPALDILDGKVVRLSQGDYTSAKIYSEHPVDLAFHFADHGA
                     ERLHLVDLKGSKEGKICEGRLFADIR"
BASE COUNT         1995 a         1832 c         1678 g         1975 t
ORIGIN      
        1 aagagttgct cgacgatctt gtgccgaagg acttgaaggc gaacacctcg tccgatatat
       61 ttagccgcac acccgcacgg gaaaaaatta taacgcatat cggcaataac cccgaacgcc
      121 aaaaaatgtt cgaggcggcg cggcagtttg aaaatttttt cttagaaaaa atgtttcgcg
      181 aaatgcgcaa gaatatgggg aaagacgata ttctgcacgg cggcgcagcg gaaaatattt
      241 ttcaagatat gcttttaacc gaacgcgtca atacgctcac tcaaagcgca accttggggc
      301 tcgccgaaca aatttatact caaatgcaac gcactcagtc atgaggaaat cccacttccg
      361 attctttatc taccttttta ttttttcaat cgtttcgtgt ttgtacgcac aagaaccttc
      421 ttcgactcca gaaacaaaac ctccaacaac cattcacaca tctccttcca ttttcaaaag
      481 tcctgcgctt aaacgcgcgg agaaatttta tttctcaaaa aaatatgaaa ctgcggcggc
      541 gttactaaga aagataatag acgatgaacc cgagaatggt cgtgcgaatt ctcttttggg
      601 tgacgtctat tatttacagg gacactataa aaaagcagtg agtcatcttt tacgcgccac
      661 cgaactcaac gaaagcaaag cgaatgactt tttccgttta ggccaaacat atattaagtt
      721 aaatcaatcg aaagaggcgc tatctgcatt ccaaaaagcg tacgagaacg acgacaaaat
      781 gaaaagcgca ctttttatgt ctggttttgt ttatttaagt ttagagagga ataaaaaaaa
      841 gacaattgaa ttctggcagc ggtttgtcga cgaagccaca cacgacccgc aacgcgagaa
      901 agtcgccacc gtgctcaaat acttgagcga ccctaatttt gtattaccaa ataaaaacag
      961 ccccatctcg ctcgaagagg ctcttctcat cggcggtaaa gcaggtcctg ctgagactaa
     1021 aaccggggaa gataaagggg ctggtcatga aaacgccaaa gagaaaaatg actctaaaga
     1081 acttctcgac gacgacagcc taaactaaaa agcgggcaat gccacaagca aacgatcgtc
     1141 gcagtcaaca ccgcgtcacc gccggtgcct atatcgaata ccgtgtcacc atcgattcaa
     1201 cccaggtcaa agacgccatc atgagcgata tttccgaagg cggcctgatg cttcttgtcg
     1261 aagaggccga tttaggccgt aaattcaata tcgacgatgc cttgtccggt cacattatcc
     1321 acgaccgcac gaaatttaat tttccataca ccggtaaagt cgcatggctg caaccgcgcg
     1381 aaatgcgcac tcgcaaattt gtcgcgatgg gcatccgctt tgacgacggt atcgaactcc
     1441 ccgcgcaaat ttatgcaatc ctcatggcag accacgattt ttaaattcgc taaacgccta
     1501 ttaccgttgt ttcaaataag taaagccatg ctcaaaattt gtggcggatg gcttacagtg
     1561 ctgtctttaa tcgtcattct caccgttttt ttgcagcatt tacgcaaagg ctccgctaaa
     1621 gaagtgttga tgggacaacg cgcctcgcgg gctgcgctta aaaatcttaa agaaaaatct
     1681 ttcggtacaa aatttaacga gtttgtagac gatctgccca acctcttaca ctggaaaacg
     1741 ctgcgcggta caaagctcat cccgcagatt accgatgggc tccaaaacac tgctcttgtt
     1801 tcgggcttaa gtttgctttt tgcccttggc atcggtttta tttttctcat caccgccgag
     1861 ctccatccga aaactgcgct tttcttagac cgttttgcca attcgataaa caccgtaccg
     1921 atttttcttc tcggtatttt ttttatttgg ctctttgctt tcgctctgcg tcttttaccc
     1981 gcaggcggcg atcaaaattt acgagatttt attttgcctg cgctttctat tgccaccaag
     2041 tttggcgcgc gcctttttct tttactcaaa gagtacatga acgaactgga aagaaaagtt
     2101 tttattctgc gtgcgcgcgc atttggatta agtcgaaagc gcatctttac tcacaagcta
     2161 ctaaattgcc tcatgccttt tattattttc tggctcatcg agacggcgtc gcttttttcg
     2221 ggagcggtga ttgtcgaaac acttttttct atccacggcc tgggttcgtt actccttttc
     2281 gcacttttgc aatacgacat tcggctcatc tttgttaacc tcgcagtgat tgccacaatc
     2341 gtttatgcca cctccatttt acaagcgcag ctttcccgtt ttcaagagag cgctcagtga
     2401 aacgtatact cagactttat tacgcaccca gcattgtggg atgccttctt ctcttttctt
     2461 tattcttacc caaaaatgaa atccaattag agaatgcatt tctttcggct tcgtggaatg
     2521 ctcccttagg cacgacgccg ttgggtgaat ccattttaac gcaggtactt gtgggcgcag
     2581 gccaaacgct caccctctcg tttgcagcgc tgagtgtgtg tttaactttt agtctcacaa
     2641 tcggcgggat tttatttcaa ctcccccgcc ccttgcaaaa ggcgtacaca ttttttatcg
     2701 acgcgtggct ttcaataccc agtattttta tcgccctttc tatcggatac tttttaccgc
     2761 aaagtttctt ttctgttttt gtggcgcttg tgctttcgga gttcgccgcg ctacaaaaat
     2821 ttttcgtgca gcggctacaa gaaactcaat cgagcgattt tttgaccgct gcgcgcgtta
     2881 tgggggcgtc gaaagcgcac ttagcggcaa agcatatttt gccccgcctt gttcgtgatg
     2941 cgctctattt attcatcatt accctgccgt cgattgtact ctccctcgcc agtcttgaat
     3001 ttttgggcgt acaaaccggt tcgtcgacgc tctcgttggg tatgcaaatc gctctctaca
     3061 aagattatat ttttttatac ccaaccctca gccttgcgcc ggtgttgtgt atattgttta
     3121 ttctcttttt actcaacgaa acagcgaaag tatcacgcgc ttaaaaccga agcggacatg
     3181 ctataatttt gaaagcgatc aacacaaatt ttctttagac attccgcttg ggctcgaact
     3241 gtaaataccg ctcaaccaag ctgatattct tgtcgctatc gcggcggcgc ggcggcatta
     3301 tcgtttcagg catacggctc atcgaatcta acgtgcgtaa aaggttcgag cggaaatcgg
     3361 caataatccg ccgtggcaat gcaaatgcag tgcctttgtg gattgtcttc aaaagtttgc
     3421 gcgcttcgcc ggtgtcgatg agacgacgcc gcaataagtc ttcggtagaa acggtaaact
     3481 cattcgcgcc aaaattataa ttgcggatca tgtcagaaat aacgcggaag agccccgctc
     3541 gcgtaatcac gtcggactta tataaaataa ccgctgtttg aaaataattt tgggtgggga
     3601 tcaccttttc cgaaagagtg gtatgtaaac tggcggatgt tttttgttct aagtgaataa
     3661 agatttgccg caccgcttcg cgcggcttgt aaccgctatc ggcaagacgc ttcagattat
     3721 cggcaataag cttcatttcg tcttgcgcga cttgccattt tttaagtatg gtgagcactt
     3781 cgtaatactc aagtttgccg gcggcaatat cgtcatagag tgaataaatt gccgcatcgt
     3841 attctgaatc gtcgccaaag agaatctcct cggcacgcac gggaaaaatc ccccgcgcat
     3901 aaattaaagc ggcgagctta aagccaattt tatcgataat ttttttgaac tggaatttac
     3961 gcaccgcagc gcgccaattc ttgaaaagaa taccgtcgaa cgcaacgccg tcgagcttca
     4021 atttttcagc gacaactttg tgcagcccct cgggcgaacc agaaataaaa taaatcggca
     4081 ccggtggttt ggtgctgtcc aaccctctgc ggatttcgcg taccaccgcg gcgacgccag
     4141 gtatattttt tttctcttcg gctttttcaa agggaatgcg cacaagttta cggaaagact
     4201 cgaagcgggt gtcgagatag gttttatcta aatcgaaagt acacaccaaa ccgtcgtact
     4261 tggagttact acgtttcact ccatacattt gttcgagaga cttaagcatc gggtacaaac
     4321 aaatggatga gatgatagaa gaacaaggca aaaagtccgc ttatggggat agtgataatc
     4381 cacgcccaaa aaagctttac cgtaatgccc cagcgcaccg agctaatccg cttcgcaagc
     4441 cctacaccga tgatgctacc agtgatggtg tgcgtggtgc tcacggggat accaaattct
     4501 cctgtggtaa aaagcgtcac tgcaccggcg gtttcggacg cgacgccctc aaacgcggtc
     4561 agttttgtaa ttttagtgcc catcgtttta acaatacgcc atccgcccat gagagtaccg
     4621 gctccaatgg cagagtaaca actaattacc acccactgcg gcaattcggt caaaacttga
     4681 attttacccg agacaagcat cgccgcgccg ataatcccca tcaccttttg ggcgtcgtta
     4741 ccaccatgcc ccaacgaaaa cgcagcagac gataataact gcaatcgacg aaaccagcgt
     4801 tcagcgcgca tggggtgcgt gtttttacaa atatgcaata gaataatcga gttcatgtac
     4861 gacatgatca tacccaaaaa aggcgcgagt acgatgaacg ctaagattgg ccaaattttt
     4921 gccattttaa cgacgccaaa tcccgcgtgc gcaacggcgg caccggcaaa gccgccaatg
     4981 agcgtgtgcg acgaactcga agggatacca aaataccaag tgatgagact ccacnnnnnn
     5041 nnnngatggc agccaacaag cccgccatga taacaaaaag ggtgatggcg ctcgtatcga
     5101 cggttttggc gacagtgtcg gcgacgtggt gctcgaaaat ataatatgct agaaagttaa
     5161 agaatgccgc ccaaatcacc gcttgcaacg gcgataaaac acgcgttgaa acaatcgtcg
     5221 caatcgaatt ggcggcatcg tggaagccgt taataaaatc aaaaagtagc gccagaataa
     5281 taacgacaac aaacaattcc atcttatgaa tacttaataa cgatactttc catcacgtct
     5341 gccacgtctt cgcagctatc ggtagcggtt tctaaaacgg caaaaaactc tttcatttta
     5401 atgaggttga tggcattttt ttgtttgaga aaaagatctt tgatggcggc attgaagatg
     5461 tggtcggctt ggttctctaa actatgcacc tgcaccaaca gttcggtgat cttgtgacgg
     5521 tccttgagat tcataagaag cggcgttgct tgcgctaaaa tttcaatttg ttttttgagt
     5581 actttagcaa tctctaccga cggccctgga aaattagcaa tatggtaaag gcgcgttcgt
     5641 gccgccgcac cttggatgtt atcggcgatg tcgtccatcg acgaggcgag cgcgtgcacg
     5701 tcttcgcggt caaaaggggt gataaacgat tcttctaatg caacgtagat actatgagtg
     5761 atatcatcgc ccacgttttc caaacgatcg atctctttga cgagtttttc tcgttgcttc
     5821 gcatttttct ccgccatgag ttgagtcaaa gcatcggcta attggattag gttatcggta
     5881 tcttgcttaa agagcggacc gaatgtgcgg tctttggggg ataaaaatcg gaaaaagcta
     5941 gtcagcgaca tatatacctc tctacgaccg aaatgggatg tccttcgtat cgtaaagtcg
     6001 tttgacaagg tgttttattc acaggttcct caaagcgcat gcgaaccgca caaattgaaa
     6061 gaaaaactaa cgaaaccgcc atacgcctcg cgttgaattt agacggcact gcaaaaatga
     6121 cgggagaaaa cccgattgct ttttttgagc acatgctcgg ccacattgta aaatatagta
     6181 tgttcgacct cgacttacac ctcaagggcg atttagccat cgactgccac cactcggtcg
     6241 aagataccgg cattattttg ggtgatgcgc tccgtcaagc gttagggaat aaaggaggaa
     6301 tttttcgtta tggctnnnnn nnnnnagggc atccgccgct ttggccacgc ctatatcccg
     6361 atggacgaga cgctcaccac cgtgactgtc gatttatcgg ggaggccata ttttgtgtac
     6421 cgtggcgaac ccttaaaacc gatgggtaaa ttcggcattt acgattcaga gctcaccctc
     6481 gaatttttgc ataaactctc gatccatgcg gcgatgaatt tgcacgtcca agtgcattac
     6541 ggcgaaaacc gccatcacat ccacgaatcg atattcaaag cactcggatt tgcgctgcgc
     6601 caagcggttg cgatcgatgc ccgccgtgca catgaaatcc cctcgacgaa gggtacactc
     6661 taagacacga tgcacaaaag ccaagcgcgc attgctttga tcgacttcgg tatgggtaac
     6721 atccgctcgc tccaaaaagc atttgagttt ttggggcatg cggctgtcgt aacaggcgac
     6781 tataaagaag cgcaaaaagc agatgtgctt ttgttgccag gcgacggcgc gtttgctcgt
     6841 gcgatgcacg gtctcaaaga gcagcacctg gacgaggtac tctacgaagc gcaccnnnnn
     6901 nnnnnaacag aaaattattt tcggcgtttg tatcggcttt caactctttt ttgaatcgtc
     6961 caccgagttc ggtggcgcaa aaggattggg actcgtcaag ggcgccatca cgcgccttga
     7021 aaaaaatgcc gacgcgcggt ggatccccca cgtgggttgg tcgacgacag tctttgaaga
     7081 agatagcctc ttaggccgtg ggatttaccc tgaggcgatg ttctattacg tgcatagtta
     7141 tgcccaccgc gcacaacacg aatacgccgc cgcaacgtgc gactatggcc aaatttttac
     7201 ctcggttgtg gaacatgaaa atttattcgc agtgcagttc caccccgaaa aatcgcaccg
     7261 tgcgggtctt aaactcatcc aaaatttttt agaggtgtta tgaagctcat ccccgcatta
     7321 gatattttag acggcaaagt ggtgcggctt tcgcaaggtg attacaccag cgcaaaaatt
     7381 tatagcgagc accccgtcga tttggcattc cactttgccg accacggcgc cgagcggttg
     7441 catttggtcg atctcaaagg ttctaaggaa ggtaaaattt gcgaagggcg actttttgcc
     7501 gatatacgcc
//
LOCUS       DAOMPD010000059         7483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4239_length_7483_cov_2.952208, whole genome shotgun sequence.
ACCESSION   DAOMPD010000059 DAOMPD010000000
VERSION     DAOMPD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_7483_cov_2.952208"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLY93_03235"
     CDS             <1..573
                     /locus_tag="PLY93_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HRP68522.1"
                     /translation="ATFDLYTVRGYTARELWQMGREEMGGGPGGGGGEPAPLGLIAFL
                     TPSTFKGDFGGSTLADSLCESFKPPIASGKSFKAFVFDSSRSALSAVLQASTTYYQPD
                     GQTPWFSTDGFLQFTSIDTPLSGAPKFWTGLVNGNPGIGDACQNWSDSTILYNGYTWD
                     AAAIETSSFTTGTPETCDSDRSLLCIEQLP"
     gene            complement(640..3348)
                     /locus_tag="PLY93_03240"
     CDS             complement(640..3348)
                     /locus_tag="PLY93_03240"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRP68523.1"
                     /translation="MPKKPIKKTNIAQRKSSSVRKENNKRERNNTGRDSKEAATGPII
                     RTLPQSFSGTTESGTQPKRKLWKLALKIVIVLLLVTAFYLIYKVRASVIEETGVKKIE
                     GVHSRVTIARDSMGVAFVKADNEDDAFFGAGYAAATDRLWQMYSTKLAVTGRLSEIAG
                     SKMLPVDVYMRTVGIARNVERAVKKMPPEYIKPLEAYAAGVNAFLKTHKNLPIEFVLT
                     GYTPEPWTVSDCGYVFGALSFGLALNINEELFFLDAAKTLGAEKAAWLVPTYQDEPIP
                     FAEAQKIDKKLAGALAPHADKITQALVALNDVLRVGMPASNNWAVSGEKAKGGKAIIA
                     NDTHLPITMPSIWSMMSIESPTYKAAGVTVAGQPYVQLGYNGKIAWGATMVEADCQDL
                     FIETLKTENGQTFFLRADNTWSPVRERKEHFKVRFGRDRDVTLRFTANGPLISDALNQ
                     LDVTPHTPLVPVGWAHGGAKADGATDGGTSGRTLQHLAMRWSLQDGDTAGIGIYEMGC
                     AHTMENFRSALSKMHSMYLNMVYSDGAHIAWQTTGKIPVRTKGTGQLPSIAADGYRWK
                     GFLPFERQPHKENPESGFLVTANERTVDKDDKRQISGVWYSPERSERIADVLSKKNDW
                     DADATKKLQLDLYSRMAEKTQFMFKAAGHDLNEAIAKLPEKKQKRAHSALAVLAKFDN
                     VMTKDSKGAAIIAAFYHAFTRNVFGDELKLNSSLWKNFIDTNLRSYNAPQDHILGREE
                     SPFFDDIRTEAKETKADMVALSLADAYEFCEDEMGSPDKWRWGKLHKIYWRADITKDL
                     PLSAWYFNYGPVAYQGDSHTVNVAHYAWGENFDTYVIPAMRFVVDFNRTEPAELIVHT
                     GVSGHPSSEHYNDQLKYFLEGKNHPLPMGEAAKMQQYKKVLVLEKL"
     gene            complement(3388..3460)
                     /locus_tag="PLY93_03245"
     tRNA            complement(3388..3460)
                     /locus_tag="PLY93_03245"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3425..3427),aa:Asn,seq:gtt)
     gene            3687..4265
                     /locus_tag="PLY93_03250"
     CDS             3687..4265
                     /locus_tag="PLY93_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68524.1"
                     /translation="MGSSHKKVKFFFQFWNIGTFLPLRVFFSLLFPLLLTSNSLQAEK
                     SARRQELQYYRNEFEARYNYPIYDTLFEKIIWERKVSHIAMGIHGQEYRVTLLMMQEY
                     PWNFKSKNYPIHFTFKSFKDAADKFHEMNHFLRNNGVMAVGLKGDTIIHEKILVAPVG
                     NTPARPSLTDKPGLKDTDPILTRQPLPRRLPL"
     gene            4262..5074
                     /gene="truA"
                     /locus_tag="PLY93_03255"
     CDS             4262..5074
                     /gene="truA"
                     /locus_tag="PLY93_03255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRP68525.1"
                     /translation="MKIALTIEYDGAPFYGFQIQNHQDSVQAQLEKALYTVLRQNVRV
                     YCAGRTDTGVNALGQVVHFATENVPPMQRLVYALNSILPRTIAVRHGALVPEDFHARF
                     SCTAREYVYMIYNAPYRSTTLGYKVLWLRDALNWDFVRDAIPHLVGERNFAAFTRVAL
                     VKSGEKTRRRIDAIHVVVKGRMVYIHICGSGFLHNMIRILVGTLVDVARGKIQPEKVA
                     SIVASANRLEASVTLPPNALYFLHAQYNETDYNQSEAQHDLRKWVIENSGDG"
     gene            complement(5091..6404)
                     /locus_tag="PLY93_03260"
     CDS             complement(5091..6404)
                     /locus_tag="PLY93_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68526.1"
                     /translation="MGLASSQEGMRGMKKIPLTLTFFSLFFLYCSAQSERNPTVVAAE
                     ENARKKPLLNPPSGVYYGALNVQVLDESGAPLEYSTGGNFQLLVGNNLNFTESTTVVV
                     RNANNTALSTIETYAISSTFATLSADPPPGVYSAVQLVSLYAPQKNVSIEVYNGSTFE
                     AYNGEQGITIPATRNVIVRQCVAGNCSANITLNYQINIPGSPPPAPRALTEAEIATFV
                     SAAGNTYKQIGSAGLAAVLDDAKLLVINTPSLIPSESLRQRFLGQDNQATAQENCTTI
                     ARYLYVMARRITLEGHTLPAFPDYSRYFIDQIVEGNLTVDSGGRNFVWVANGAALVTP
                     YLPADALSPYDYQRGTTYPNDFSILDKLVSDNPFATLLRDGPSASSNTHTFMAIKSTA
                     GYTMIDTYFTPFNGVETRASAGGAYPYAYRFGPNGSRWLHFVYGY"
BASE COUNT         1888 a         1815 c         1793 g         1987 t
ORIGIN      
        1 gccaccttcg acctctacac cgtgcgcggc tacacggcgc gtgaactttg gcaaatgggc
       61 agagaggaga tgggcggggg acctggcgga ggtggtggtg aacctgctcc tcttggactc
      121 attgcattct tgacgccaag tacattcaaa ggggattttg gtggctcaac acttgccgac
      181 agtctttgtg aaagctttaa accaccaatt gccagtggaa agagttttaa agcatttgtt
      241 tttgattcta gtcgttctgc cttaagcgca gtcttgcaag caagtacaac gtactatcaa
      301 cccgatggac aaactccatg gttttcaaca gatggattct tacaatttac aagcattgac
      361 acccccctat ccggtgctcc caaattctgg acagggcttg taaatggaaa tcctggaata
      421 ggagatgctt gtcaaaattg gagcgactct actattttat ataatggtta cacttgggat
      481 gctgcggcta tagaaacctc cagttttacc acagggactc ctgaaacttg tgacagcgat
      541 cgatcactcc tctgcatcga gcaactacca taaattttaa ctttgaggct ggctaacaag
      601 gcgtaagcga cttattagac agccactcct tgttcactgc tataacttct ctaacaccaa
      661 cacctttttg tactgctgca tctttgcggc ctctcccatc ggcaacgggt ggtttttacc
      721 ttctaaaaaa tatttgagtt ggtcgttgta gtgttccgac gagggatgtc ccgacacacc
      781 ggtgtggaca atcaactctg caggttcggt gcggttaaaa tctaccacaa agcgcatcgc
      841 ggggatgacg tacgtgtcaa aattttcacc ccacgcgtag tgtgccacgt tcaccgtgtg
      901 gctgtcgcct tggtatgcca cggggccata gttaaaatac caagcagaga gcggtaaatc
      961 tttggtgatg tcggcgcgcc agtaaatttt atgcagcttg ccccaacgcc atttgtcggg
     1021 ggatcccatt tcatcttcgc aaaattcgta tgcatcggcg agcgagagcg ctaccatgtc
     1081 ggcttttgtt tcttttgctt cagtgcgaat atcgtcaaaa aacggacttt cttccctacc
     1141 taaaatatga tcttgtgggg cgttgtacga gcgcaagttg gtgtcgataa aattcttcca
     1201 caaggaacta tttaacttga gctcgtcgcc aaatacattg cgcgtgaagg cgtgataaaa
     1261 cgccgcgatg attgccgcgc ctttcgagtc cttagtcatc acattgtcga attttgctaa
     1321 aacagccaag gcagaatgtg cacgtttttg ttttttctct ggcagttttg cgatagcctc
     1381 gtttaaatcg tgtcctgccg ctttgaacat gaactgtgtt ttttcagcca tgcgtgaata
     1441 taaatcgagt tgcaattttt tcgttgcgtc ggcgtcccaa tcgttctttt tagaaagaac
     1501 atcggcgata cgttcggagc gctcggggct ataccacacg ccgctgattt ggcgtttgtc
     1561 gtctttatcc accgtgcgct cgttcgcagt gaccagaaaa cccgactcgg gattttcttt
     1621 gtgcggctgg cgctcaaatg gcaaaaagcc tttccagcgg tagccatctg cagcaatcga
     1681 cggcaactgg cctgtgcctt tggtgcgcac gggaattttg cctgtggtct gccatgcgat
     1741 atgggcaccg tcgctataga ccatgtttaa atacatcgaa tgcatttttg agagagcgct
     1801 acggaaattc tccatcgtgt gcgcacatcc catttcgtaa atcccaatac ccgctgtatc
     1861 gccgtcttgc agcgaccagc gcattgcgag gtgttgtaaa gttcttcccg acgttccgcc
     1921 atccgtggct ccgtcagcct tagcgcctcc atgcgcccag ccaaccggca ctaagggcgt
     1981 gtgcggtgtg acatctaact ggttgagcgc atctgaaatg agtgggccgt ttgcggtgaa
     2041 acgcagcgtc acgtcgcggt cacggccaaa gcgtacttta aaatgctctt tgcgttcgcg
     2101 caccggcgac catgtgttgt ctgcgcgtaa aaaaaatgtt tggccgtttt cggtttttaa
     2161 ggtttcgata aaaaggtctt ggcagtccgc ctcgaccatc gtcgctcccc aagcaatctt
     2221 tccattatac cccaactgca catacggctg gcctgccaca gtcacacccg ccgctttata
     2281 agtgggggat tcaatactca tcatcgacca aattgacggc atggtgatag gcaggtgcgt
     2341 gtcgttagcg ataattgctt taccgccttt ggccttctct cccgaaaccg cccagttatt
     2401 cgaggcgggc atccccacgc gcagcacatc gttgagcgcg acgagagctt gcgtaatttt
     2461 atcggcgtgt ggggccaaag caccggcgag ctttttgtct attttctgcg cttcggcaaa
     2521 agggatgggt tcgtcttggt aggtgggcac aagccacgcc gctttttcag cgcccaacgt
     2581 tttcgcggcg tctaagaaaa aaagttcctc gttaatattg agcgcaagac caaacgaaag
     2641 cgcgccgaaa acgtaaccgc aatcgctcac tgtccacggt tcgggtgtgt acccggtaag
     2701 aacaaactca atcggcaagt ttttatgtgt cttcaaaaac gcatttacgc cagcggcata
     2761 cgcttctaat ggtttaatat actcaggcgg catttttttt acagcgcgct cgacattgcg
     2821 cgcgatgcca acggtgcgca tatacacatc caccggcagc attttactgc ccgcaatttc
     2881 agaaagccta ccggtgactg caagctttgt cgaatacatt tgccacaatc gatcggtcgc
     2941 ggcagcatac cccgcgccaa agaacgcgtc gtcctcgttg tcggctttca caaacgccac
     3001 acccatgctg tcacgcgcaa tggtgacgcg cgagtgcacg ccttcaattt ttttcacgcc
     3061 cgtttcttcg ataaccgacg cgcgtacttt atagataaga taaaacgcag taacaagaag
     3121 tagtactatc acaattttta gcgcaagctt ccacaacttt ctcttgggtt gagttccgct
     3181 ttcggtggta ccagagaacg actgcgggag tgtacggata atcggccctg tcgcggcttc
     3241 tttagaatcg cgacctgtat tgttccgttc acgcttattg ttttctttgc gtactgagga
     3301 ggattttctc tgcgctatat ttgttttttt tatgggtttt ttgggcatgg tggtatcgtt
     3361 cgagattagt gaataaacga caaagtgctc ccggagcagg actcgaacct gcgacaaggt
     3421 ggttaacagc cacctgctct accgactgag ctatccggga atggatagtt tacccttcct
     3481 aacagcgcct ctctacagac tgtgaccgta ggtctccgtc ttcggtgcta tctgggaata
     3541 gatagtttta taagtacaat tcaattattt tgcgtacgca aaataattaa atataacata
     3601 tagtcttcgc ggaacaaacg gagatttcta caccgcgtca atctctattt gcttacctct
     3661 ttgaggaata aatccgaaaa tagagtgtgg gttcatcgca caaaaaagtg aaattttttt
     3721 ttcaattttg gaacattggt acatttctac ctctgcgcgt atttttttct cttttattcc
     3781 cgttgttatt aactagtaat tctcttcagg ctgaaaaaag cgcccgccgc caagaattgc
     3841 agtactatcg taatgaattt gaggcgcggt ataactatcc tatctacgat acgctgtttg
     3901 agaagattat atgggaacgc aaggtctcgc acatagcgat gggaatccac ggccaagaat
     3961 accgcgtaac gctactcatg atgcaggagt acccgtggaa tttcaaaagc aaaaactatc
     4021 caattcactt tactttcaaa tctttcaaag acgccgccga taaattccac gagatgaatc
     4081 attttttacg caacaatggc gtgatggcgg taggcctcaa aggcgacacc attatccacg
     4141 agaaaatatt ggtcgcaccc gtcggcaaca cacctgcacg cccatcgctc accgataaac
     4201 ccggtttgaa agacaccgac cccattttaa caagacaacc gttgccgcgc agactgcctc
     4261 tatgaaaatt gcactcacta tcgagtacga cggcgctcct ttttatggct ttcaaattca
     4321 aaaccaccaa gactctgttc aagcgcaatt agaaaaagcg ctttataccg tcttgcgtca
     4381 aaatgtgcgt gtgtattgcg ccggtcgcac tgacacgggc gtcaatgcgt tggggcaagt
     4441 ggtccacttt gccactgaaa atgtcccgcc gatgcaacga ctcgtctacg cgctcaacag
     4501 cattttgccg cgtacgattg ccgtccgcca cggcgcactt gttccagaag attttcacgc
     4561 acggttttcg tgtaccgcgc gcgaatacgt gtacatgatt tataacgcgc cctaccgctc
     4621 gaccacttta gggtataaag ttctttggtt acgcgacgcg cttaattggg atttcgttcg
     4681 tgatgcaata ccgcatttgg tgggggaaag aaattttgcc gccttcaccc gcgtcgccct
     4741 ggttaaaagt ggagagaaga cgcgccgtcg tatcgatgca atccatgtcg ttgtgaaagg
     4801 ccgcatggtg tatatccaca tttgcggatc gggctttttg cataatatga ttcgtatttt
     4861 agtgggtact cttgtcgatg tagcgcgcgg caaaattcaa cccgagaaag tagcgtcgat
     4921 tgtcgcgtcg gcaaaccgac tcgaagcgag cgtcacgtta ccgccgaatg cgctttattt
     4981 tttgcacgcg cagtacaacg aaaccgacta taaccaaagc gaagcgcaac acgatttacg
     5041 taaatgggtg atagaaaatt ctggggacgg ttaatgggcg gccaaaactc ctaataaccg
     5101 tacacaaagt gcaaccaacg cgacccgttc ggaccaaagc ggtacgcata cggatatgca
     5161 ccgcctgcgc tggcgcgggt ttcaacgccg ttgaaaggcg tgaaatacgt gtcgatcatt
     5221 gtataccccg ctgtgctctt gattgccata aacgtatgcg tgttcgacga cgccgaagga
     5281 ccatcgcgca agagcgtcgc aaacggatta tcagaaacca gtttgtcgag aatactaaaa
     5341 tcattggggt atgttgttcc gcgctgataa tcgtaagggg agagtgcatc ggcgggtaaa
     5401 tagggagtca ccaacgccgc accgtttgca acccacacaa agttgcgtcc accagagtcg
     5461 acggtgagat taccttccac aatttgatcg atgaagtaac gcgagtaatc ggggaaagcg
     5521 ggtagagtgt gcccttcaag agtaatccgt cgcgccataa cataaagata acgcgcaatc
     5581 gtggtacaat tttcttgggc tgtcgcttgg ttgtcttggc ctaaaaaacg ctgccgcagc
     5641 gattcgctgg gtatcagcga tggggtattt atcaccaaaa gttttgcgtc gtctaaaact
     5701 gcggcaagtc ctgcgctacc gatttgtttg tacgtgttgc cagcggcgct cacgaacgtg
     5761 gcgatttccg cttcggtgag cgcacgcggt gcgggtggcg gcgaacccgg aatgttgatt
     5821 tggtagttga gcgtaatatt cgccgagcag ttacccgcga cgcactggcg cacgatgacg
     5881 ttgcgcgtgg cagggatagt tatcccttgc tcgccgttat acgcctcaaa cgtgctgcca
     5941 ttatacactt caatactcac gttcttttgc ggtgcgtaca acgatacaag ctgtactgca
     6001 ctgtaaacgc ccggaggtgg atcggcgcta agcgtggcaa aggttgaaga gatcgcatac
     6061 gtttcaatgg tcgataatgc ggtgttgttg gcattgcgca ccaccacggt ggtgctctcg
     6121 gtgaaattca agttattccc gactaaaagt tgaaagttgc caccggttga gtattctaaa
     6181 ggcgcgccac tttcatccaa cacttgcaca ttgagtgcgc cgtaatacac accgctgggg
     6241 ggattgagga gcggtttttt ccgcgcattt tcttcggcgg cgacgacagt ggggttgcgc
     6301 tcactttggg cggagcaata gagaaaaaac agagaaaaaa aagtcagcgt tagtgggatt
     6361 tttttcatac cacgcatacc ctcttgtgag gatgctaatc ccatgtgtgt atcttgtcaa
     6421 gataaattaa aaattttagt atgcaacttg aatctaagga gcctgtggca tgagccgggg
     6481 gtgtcgcctg attgtaccta tatcagctcg cagcacccaa acttcccaat cgatccacaa
     6541 acaatcgatg ctttccgcac acctttccaa acaaggcgaa ttggatataa agttttcaaa
     6601 gcaaaatcag actgtttttc accgtcacac taaatctaga agctggcgaa taaaactggc
     6661 ctactcaatt tttttctgtg ataaaaagca caatttttga acgaaagttt cattctctga
     6721 gactatactt acacattcaa gggcgatgac acttcaattt gcaataaccc gctaattctg
     6781 gcgggcgcga tccgggcggg aaaaatttcg actgccaacc tttgtcctct ggggaaaaaa
     6841 tttacttcaa tgcatgacat gaaaactgac gccaaacggc ttttaataaa atgtaaatag
     6901 gctcaagcta ttgattatgg ggctcatttt aagcctcata attggtggtg actaaaattc
     6961 aaaaaagaaa atcaatatga aaaatttgcg atttttgata gtacttttgg ccacaatcag
     7021 tttgtttggt cagtccacaa ccattactgt atctggaacg ggaagccatc cagatgggag
     7081 tggaagtgaa ggtaccgcac agttttagta caggaaagaa acttcatata aatatatgga
     7141 tgcctgtaag gataacggtt atcgaggcgt tcagcacaga acacagtctg gagaaaatgt
     7201 tatatttcat attgcgggaa gaagacgaaa agctttcaaa acgcaaaatg ccgaatgtcg
     7261 cgatttagga gggcaaacac aattgaccgg atactaccga attattgaaa tatacaaata
     7321 atcttcatct aagtcaatag ggaaaaacat aaatttattt gattttttta gctatccatg
     7381 gacgacaggc tcgaaggagt tgaaaccgcg cgcttttgcg ttgcgggcgg tttcccccct
     7441 gtgtcagcgg attctgtcgc ctttaattga ctcgtaattt gat
//
LOCUS       DAOMPD010000060         7463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4254_length_7463_cov_2.825594, whole genome shotgun sequence.
ACCESSION   DAOMPD010000060 DAOMPD010000000
VERSION     DAOMPD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_7463_cov_2.825594"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1024
                     /gene="dinB"
                     /locus_tag="PLY93_03265"
     CDS             68..1024
                     /gene="dinB"
                     /locus_tag="PLY93_03265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRP68527.1"
                     /translation="MPFRTIVHVDMDAFFASAEILDHPELKNKPVVVGADPLGRQYNG
                     KQAMEIQSNSEGIARTRGVVCAASYEARRFGVKSAQPVKKAYQLCPHAIFLPPRFERY
                     SELSDMMVAALGVFSDTVLRLSIDEAVLDCTELGSRFGTWRTLAEGIKKEVAQATGLT
                     CTVGIATGRTLAKMASEINKPDGLCLIRPGKEKEFLAPLKVESIPGVGPRAKETLNRI
                     GIRTVEDLQKIDIHTLVALLGKWGARIFEIAQGIDNESVVGVRERKSFGEECTYEKDL
                     SSAVLYGGDGSALGFRYPRRSGERDGAQNDFRAHYHSQNPLC"
     gene            969..1220
                     /locus_tag="PLY93_03270"
     CDS             969..1220
                     /locus_tag="PLY93_03270"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68528.1"
                     /translation="MERKTISGRTITLKIRFANFHTITRSQTLPVPLKDAASLYGEAL
                     QIFLRSVTREEIQHEKIRMLGIQLSHLYSARVGEQLWLF"
     gene            complement(1212..1925)
                     /locus_tag="PLY93_03275"
     CDS             complement(1212..1925)
                     /locus_tag="PLY93_03275"
                     /inference="COORDINATES: protein motif:HMM:NF021449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HRP68529.1"
                     /translation="MPLKKMPSVRESDLYPQAAAYFMRQGFEVKGEVCSCDLVAIKDD
                     TIIIAELKLTFNIKLLYQAVRRLMITEQVYVVIPKPKKRQKMSYWQMMKSLCRRLHLG
                     LLIIDNDDVLVLAEPKPFNAKISARHKKRVFKEFHGRKVAQNAGGTTGIKLETAYLEA
                     AVHISILLKKHKALKPKELVALGTTDKTARLLYNNHYGWFEKNIDGAYKLKRGKAKII
                     KNENPRIWDYYTAQVSASE"
     gene            complement(1929..5045)
                     /locus_tag="PLY93_03280"
     CDS             complement(1929..5045)
                     /locus_tag="PLY93_03280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP68530.1"
                     /translation="MEEGLIANLRDLLHALPLTTYNRAHDYMMSGHVGPISQDENYYF
                     ANVVGAEIYQTGINADTLRTHCNCPAPEPCKHVGALAMEILRTATTKEEEKSPEENIV
                     GDRELGVRFKGTNFVSFEVVSPNGETRPLTPANVSLLKPEIKKVVQKRAIVRVTSSYI
                     YTSAFVDRHLSYLKDLQGIHYFFDNEKESAEYGGELNVEFSLEEIREDNAPKSHRPDA
                     DIRELYFGIQYFVVDPVTGKRVDSIYLSHIKSIYRAENIFYIVNPCTNIEQYVHDRIS
                     LTQLRNQKKSPLYAKWQHAFETMATTGPKLIVSVEGVEDKKGRLRVQAQLEFAYATTE
                     AYLTNDGEGAEVKTGEKPIYCFSITHAYVTERLHFGFSNKQIVQHKTGMLIERKLRIE
                     KELLEKAKEFKFPKKGKIYVPKKEYLPFLQDFIPKLKAKEILVRINDNILGLLLKPSA
                     ELVIKPSSGIDWFSGRIVVRGVDLDDMGEFIRAVRRKDELIRLKNGDWLRVAGTELEE
                     VLRSLERLGIKPKADGSFEEMRLSQFASLEIEKKILLRTEKGAESAQEKIRKLLEINT
                     PSITLGTDFKATLRPYQKRGVESILKRHASGVGGILADDMGLGKTVQAIAAMSALNRQ
                     ATTSKKNASKNEFHPVRFLVICPVAALGVWEDEIQKFASNLTVYRWHGTRQEKAMRAA
                     TVVLTTFSTYSLDVKKLTQEKWTMVFVDEAQFAKNFRTRIAQNLRNLNAEAIICLTGT
                     PLENYLEDLWALFDLFFPGYLGTQKSFKDGFGGHLDVRDSEGLERRIRPFMLRRRKVD
                     VLKDLPAKTESIIRIPMTAAQARVYEAARQLALEYLKKGGSALFEMLKQLTLLRRIAC
                     HPFLETESPNPLDSGKFEYLDSKLVELSISSEGVLIFSQYTSVLKVLMTQMKRRGIEP
                     YYLDGKTSTKERRSLVARFQDGGAKFFLISLKAGGTALTLTRADTVIHLDPWWNPAVE
                     NQATDRAHRIGQTKKVIVYKLVSEGTVEEKVLTLQGMKRELFEAVIDNTKARGKKISV
                     DDIAALLS"
     gene            complement(5052..6401)
                     /gene="dnaB"
                     /locus_tag="PLY93_03285"
     CDS             complement(5052..6401)
                     /gene="dnaB"
                     /locus_tag="PLY93_03285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRP68531.1"
                     /translation="MQLFDEEAEQVVLGSILQSPSLFEEVAQILKPDDFYYEGHRIVY
                     EAIASEIDRGEQPNAILIINSLKNTNQLERAGNRAGIMNIASLGVLVGVTTFTEKIRD
                     MALRRRLVTNFRELEKKASDTGFKLDSVLSDTESALTHLADRYSSYNIRHIKEADKEF
                     VEFLKHVTTNRDSVIGTASGFKRLDDVTGGFKGGQMIVIAARPGMGKTTFALNIALNV
                     AMDKNRPKSVAFFSLEMTRLELLLRLLCSRSFLDSQKVQKGRINNSEMTRLALAAKEI
                     YASDFYIDDSADLTTWEFKQRSRRLGRTLKQQGKDLGLIVVDYLQLMTDRERASDGRQ
                     NEVAAISRALKLIAKDLNCPILALSQMNRSIEQRGKDPRPQLSDLRESGAIEQDADMV
                     IFIHRENLGHDAPEDKKNLAEIIIGKNRAGPRENFNLAFLSDKSLFSDYEAGPTPQQ"
     gene            6530..>7463
                     /locus_tag="PLY93_03290"
     CDS             6530..>7463
                     /locus_tag="PLY93_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP68532.1"
                     /translation="MVSLPVASIVNELKVSLERTPAVLLTADTGSGKTTWLPLQLLDA
                     AWLKGKKIVMLEPRRVAARAAAMRLAHHLGESVGNKVGYSVRLDSRTSAVTRLEVVTE
                     GVLARRLVNDPELADVGLLIFDEFHERHIETDLALALALESMRVFRPDLKILIMSATI
                     DTTKITTFLQNVLTTTVPCIVSQGTNFPVAIHYAGDAPQPTHFVGDNYAHVALWAARQ
                     AIQAHKLHKGDILVFLPGKSEIKTAIEFITAHNLPQTKVLPLHGELTPQEQDDALKKG
                     STLERRIIVATPIAESSITVDGLAGFGARRATAGD"
BASE COUNT         1778 a         1803 c         1808 g         2074 t
ORIGIN      
        1 cgtagatgac caggccggac ttgtgaaatc gggtgctcct gaaacatgtt ttaagtaacc
       61 gttatttatg cctttccgca cgattgtaca cgttgacatg gacgcattct ttgcgagcgc
      121 tgagatttta gaccatcctg aactcaaaaa taagcctgtt gtcgtggggg cagatcctct
      181 cgggcggcag tacaacggca aacaagcgat ggaaatccag agcaactcag agggaatcgc
      241 acgaacgcgc ggcgtggttt gtgccgccag ttacgaagcc aggcgttttg gtgtaaaatc
      301 ggcgcagccg gtaaaaaaag cataccagtt atgcccacac gctatttttt tgccgccgcg
      361 ctttgagcgt tacagcgaac tttctgacat gatggtcgct gcgttgggtg tgttcagcga
      421 caccgtgttg cgtctgtcta tagacgaagc ggtactcgac tgcaccgaat tggggagtag
      481 atttggcacg tggcgcactt tggcggaagg tatcaaaaaa gaagtcgccc aagcaacggg
      541 gctcacttgc accgtgggga ttgccacggg gcggacgctt gccaaaatgg cgtcggaaat
      601 caacaaaccc gacggtctgt gtttaatccg ccctggaaaa gaaaaagaat ttctcgcgcc
      661 gctcaaggtg gaatcgatcc cgggggtggg gccgcgtgcg aaagaaacgc ttaaccgtat
      721 cggaatccgc acggtggagg acttacaaaa aatagatatc cacactctcg tcgctctttt
      781 agggaaatgg ggcgcacgca tttttgaaat tgcgcagggg atcgataacg aatcggtagt
      841 tggtgtgcgc gaaagaaagt cgtttggcga agaatgcacg tacgagaaag atttatcgtc
      901 tgctgttctg tacggcggtg acggaagcgc cctcggattc cgatatccga gacgctctgg
      961 cgagcgcgat ggagcgcaaa acgatttcag ggcgcactat cactctcaaa atccgctttg
     1021 ctaacttcca taccatcact cgctcgcaaa cattgccggt gccgcttaaa gacgccgcgt
     1081 cgctttatgg cgaggcgtta caaatttttt tgcgcagcgt gacgcgtgaa gaaatacaac
     1141 acgaaaaaat tcgaatgttg ggaatccaac tctcgcacct ttatagcgcg cgtgtgggcg
     1201 aacaactttg gttattctga tgcactcact tgcgctgtgt agtagtccca aatacgggga
     1261 ttttcgttct taataatttt tgctttgccg cgctttaatt tatacgcccc gtcgatattt
     1321 ttttcaaacc aaccgtagtg gttgttgtag aggaggcgcg cagtcttgtc cgttgtgccg
     1381 agcgccacta gttctttggg tttcaacgct ttatgttttt tgagtaaaat gctaatatgc
     1441 accgccgctt ctaaatacgc ggtttcgagt ttaatccccg ttgttccccc tgcgttttgc
     1501 gcaactttcc ttccatggaa ttctttgaac acccgctttt tgtgccgcgc ggagattttt
     1561 gcgttaaagg gtttaggttc tgcgagaacg agtacatcgt cgttgtcgat aatcaataac
     1621 cccaaatgca accgtcggca taaacttttc atcatttgcc aatacgacat tttttgcctt
     1681 tttttcggct tgggtataac cacgtatact tgttcggtga tcatgagccg tcgcaccgct
     1741 tggtataaaa gtttaatgtt gaacgttaat ttgagctcgg cgatgataat cgtatcgtct
     1801 tttatcgcga ctaaatcgca cgagcatacc tcgcctttca cttcaaagcc ctggcgcata
     1861 aaatacgcgg cagcttgagg gtataaatcg ctctcacgca cagacggcat ttttttcaac
     1921 ggcatacact aactcaaaag cgcggcgatg tcatccaccg atattttttt gccgcgtgct
     1981 ttggtattgt caatcaccgc ttcaaaaagt tcacgcttca tgccttggag agtgagcacc
     2041 ttttcttcaa cggtgccctc gctcaccagt ttatacacaa tgactttttt tgtttggccg
     2101 atgcggtgcg cgcggtcggt cgcttggttt tcaacggcgg ggttccacca cgggtcgaga
     2161 tgaatcaccg tgtcggcgcg ggtgagggtc aacgccgtgc cacctgcttt cagactaatg
     2221 agaaaaaact ttgcaccgcc gtcttggaaa cgcgccacga gcgaacgccg ttctttcgtg
     2281 ctcgtcttgc catctaaata gtacggttcg atgccgcgcc gtttcatctg cgtcatcaaa
     2341 actttgagca ccgatgtgta ttgactgaat atgagtacgc cttcgcttga aatagaaagt
     2401 tcgacgagtt tggaatcgag gtattcaaac tttcccgagt caagcgggtt gggcgattcg
     2461 gtttcgagga atggatggca tgcaatgcgg cgtaacaggg tgagttgctt aagcatttca
     2521 aaaagcgcag agccgccttt ttttaaatac tctaaagcga gttggcgcgc cgcttcgtac
     2581 accctcgctt gtgccgctgt catagggatt ctgataatcg attctgtttt ggcgggtaaa
     2641 tctttcaata cgtcgacctt gcgccggcgt agcataaagg ggcgaatccg ccgctccaac
     2701 ccttcgctgt cgcgtacgtc gaggtgaccg ccgaaaccgt ctttgaaact tttttgtgtg
     2761 cctaagtatc cagggaaaaa taaatcaaac agcgcccaca aatcttccaa ataattttct
     2821 aacggcgtac ccgtgaggca aataatcgct tcggcgttta agttgcgtaa attttgtgca
     2881 atgcgcgttc taaaattttt tgcaaattgc gcttcgtcga cgaacaccat cgtccatttt
     2941 tcttgggtga gttttttgac atccaacgaa tacgtagaaa atgtcgtgag tacgacggtg
     3001 gcggcgcgca tcgctttttc ttgccgtgtg ccatgccagc ggtacactgt taaattcgat
     3061 gcaaattttt gaatttcatc ttcccacaca ccgagcgcgg ccacggggca gatgactaaa
     3121 aaacgaactg gatgaaattc atttttgctt gcgtttttct ttgaagtggt cgcttgtcgg
     3181 ttgagcgcgc tcattgcggc gatcgcttgc actgttttac ccaaccccat gtcgtcggcc
     3241 aaaatacccc ctacgcccga cgcatgccgc tttaaaatgg actcaacacc ccttttttgg
     3301 tatggacgca aagttgcttt gaaatctgtg cccagcgtaa tggagggggt gttgatttct
     3361 aaaagtttgc ggattttttc ttgtgcgctc tctgcgcctt tttcggtacg caacaatatt
     3421 ttcttctcaa tttctaaact cgcaaactgc gaaagcctca tttcttcaaa cgagccatcc
     3481 gccttgggtt taatccccag tctctcgagt gaacggagca cctcttcgag ctcggtgcca
     3541 gcgacgcgca gccagtcgcc gtttttgagt cgaatgagtt cgtcttttct gcgcaccgct
     3601 ctgataaatt cacccatatc gtcgaggtct accccacgaa caacgatgcg cccggaaaac
     3661 caatcaatcc ccgaactggg cttaatgaca agctcggcag acggtttaag taagagacct
     3721 aaaatgttgt cgttgatacg taccaaaatt tcctttgctt tgagctttgg gataaagtct
     3781 tgtaagaagg gtaggtattc ttttttcgga acgtatattt tacctttctt gggaaattta
     3841 aactctttgg ccttttctaa taattctttt tctatgcgta acttacgttc aatcaacata
     3901 cctgttttat gctgaacaat ctgtttgttg ctgaaaccaa aatgaagacg ctcggttacg
     3961 tatgcgtgcg tgattgaaaa acaatagatg ggtttctctc ctgtttttac ttcggcgcct
     4021 tcgccgtcgt tcgtgagata cgcttcggtt gtcgcgtatg caaactctaa ctgtgcttgc
     4081 acgcgcaatc gtcctttctt gtcttccacg ccttccacag agacgatgag cttggggcct
     4141 gtggtcgcca tcgtttcaaa cgcgtgttgc cattttgcgt agagtggcga ttttttttgg
     4201 ttacgcaact gggtgagtga aatacgatcg tggacgtact gttcgatgtt tgtgcaggga
     4261 ttgacgatgt aaaaaatatt ctcagcgcga tatatcgatt tgatgtgcga gagataaata
     4321 ctgtcaaccc gcttacctgt tacaggatcg acgacaaagt attgaatacc aaaataaagt
     4381 tctcgaatgt ctgcgtcggg tctatgactt ttgggcgcat tgtcttcccg aatttcttct
     4441 aaactaaatt cgacattgag ctcgccgcca tattcagccg actctttttc gttatcaaaa
     4501 aaataatgta tcccttgtaa gtctttcaaa taagacaaat gcctatcgac aaaggcagag
     4561 gtgtaaatat acgagctggt tacacgcact atcgctcttt tttgtaccac tttttttatt
     4621 tcaggtttga gaagcgatac atttgctggt gtcaaaggtc tggtttcacc gttgggagac
     4681 accacttcaa agctgacaaa atttgttcct ttgaaacgca cacccaactc tctatcgccg
     4741 acaatatttt cttctggaga tttttcttct tctttggtag tcgcggtacg gagtatttcc
     4801 attgccagcg caccgacgtg cttgcacggc tcgggtgcgg gacaattgca atgtgttctg
     4861 agcgtgtctg cgttaatgcc ggtctggtaa atttcggcgc ctaccacatt tgcaaaataa
     4921 taattttcat cttgcgaaat cggccccaca tgaccgctca tcatgtagtc gtgtgcacgg
     4981 ttatacgtcg ttaacggtaa tgcgtgcaac aaatctctca agtttgcaat aagcccttct
     5041 tccatttcct tctattgctg tggtgttggt cctgcttcgt aatccgaaaa taaactttta
     5101 tccgaaagaa acgccagatt aaaattctcc cgtgggccag cacgattttt gccaatgata
     5161 atttctgcaa ggttcttttt atcttcgggg gcatcgtggc ctaaattttc gcggtggata
     5221 aaaatgacca tgtcggcgtc ttgttcgatt gctcccgatt cgcgtaagtc ggaaagttgc
     5281 gggcgtgggt ctttgccgcg ttgttcaatc gagcggttca tttgagagag agcaaggatg
     5341 gggcagttca aatctttggc gatgagtttt agcgcgcgtg aaatcgccgc gacttcattt
     5401 tgtcgtccat cgcttgcccg ctcgcgatcg gtcatgagct gtaaataatc gacaacgatg
     5461 agccccaaat ctttgccttg ttgcttgaga gtgcgaccca gtctgcgcga acgctgctta
     5521 aactcccagg tggttaaatc tgccgagtcg tcgatataaa aatccgaagc ataaatttct
     5581 tttgccgcca gcgccaaccg cgtcatttca gaattgttga tacgtccttt ttgtactttt
     5641 tgcgaatcga gaaacgagcg cgaacaaaga aggcgcaaca ataattcaag gcgggtcatc
     5701 tctaatgaaa aaaaagcgac cgacttgggg cggtttttat ccatcgcgac gttgagtgca
     5761 atgttgagcg cgaacgtggt cttacccatg ccaggacgcg ccgcaatcac aatcatttga
     5821 ccacccttaa agccaccggt cacatcgtcg agacgcttaa accccgatgc tgtaccaatg
     5881 acactgtcac ggtttgtggt gacgtgcttt aggaattcga caaattcttt atcggcttct
     5941 ttgatatggc ggatgttgta actcgaataa cgatcggcta aatgggtgag cgcactctcg
     6001 gtgtcggaaa gtacgctgtc gagtttgaaa ccggtgtcgg atgctttctt ttctaactca
     6061 cgaaaatttg tcaccaaccg gcggcgcaga gccatatcac gaattttttc tgtaaacgtg
     6121 gtcactccca ctaacacacc cagcgatgcg atgttcataa taccggcacg gttgcccgcg
     6181 cgttccagtt ggttggtatt cttgagagag ttgataatca aaatggcatt gggttgctcg
     6241 ccgcggtcaa tttccgaggc gatcgcttca tacacaatgc gatggccctc atagtaaaaa
     6301 tcatcgggtt ttaagatttg agccacctct tcaaagagcg acggcgattg aagaatcgat
     6361 cctaaaacga cttgttctgc ttcttcatcg aataactgca tgggatttga ttcgctcttc
     6421 aaaaacgcgt cgcggtgtcg ataattgtgc aattccagcg cactgccgta caaagtttat
     6481 tctgctttac aatactttcc acaccgtctt aaatttctaa agtttcattg tggtgagctt
     6541 acctgttgcg agtattgtaa acgaattgaa ggtgagtctt gaacgcacac ccgccgtgct
     6601 tttaaccgcc gatacgggct cgggtaaaac gacatggctg ccgctacaac ttttagacgc
     6661 ggcatggctc aagggaaaaa aaattgtgat gctcgaacca cgccgcgtag cagcgcgcgc
     6721 agcggcaatg cggctggcac accatttagg cgagagcgtg ggtaataagg tgggatattc
     6781 ggtccgctta gatagccgca cgagcgctgt aacgcgactc gaagtggtga cagaaggagt
     6841 gcttgcccgc cgtttggtga acgatcccga acttgcagat gtggggcttc ttattttcga
     6901 cgaatttcat gaacgccata ttgaaaccga tctcgcgctc gcacttgcat tggagagtat
     6961 gcgtgttttt cgtcctgatt taaaaatcct catcatgtcg gcgaccatcg acacgaccaa
     7021 aattactact tttttgcaaa atgtgctaac gacaacggtg ccgtgtattg tttcacaagg
     7081 tacaaatttt cccgtcgcca tccactacgc gggcgatgcg ccgcagccca cccattttgt
     7141 cggggataac tatgcacacg ttgcgctttg ggcagcgcgg caggccatcc aagcgcacaa
     7201 attacacaaa ggggacatcc tcgttttcct accagggaaa agtgaaatca aaacggcgat
     7261 tgaatttatc accgcgcaca atttgccgca gacaaaagtg ctcccgctgc atggggagct
     7321 tacaccgcaa gagcaagacg acgcattaaa aaaaggaagt accctcgaac gacgtatcat
     7381 tgttgctacg ccaattgccg agtcgagcat caccgtcgat ggtctcgccg gctttggcgc
     7441 tcggcgtgcc actgctggtg acg
//
LOCUS       DAOMPD010000061         7460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4257_length_7460_cov_2.381769, whole genome shotgun sequence.
ACCESSION   DAOMPD010000061 DAOMPD010000000
VERSION     DAOMPD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_7460_cov_2.381769"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLY93_03295"
     CDS             complement(<1..954)
                     /locus_tag="PLY93_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP68533.1"
                     /translation="MAWINDNILFQTAIVTLIALVIHFAIFAILKFIAGRTRSHIDDV
                     FVDELRNPTRFLLPVILCQALLPTIQQKLSQAHHQLAVESLRALTIALIAWSIARSGR
                     ILEKIVLQKQKAQEGGDIRSVLTQIRMLRRVFSVIIFIAAAGLVLMSFTSFRQIGTGL
                     LASAGVVGLLLGVTAQRGLGNLLAGIQIALAQPIRHDDVVSVENEWGVVEEITLTYVT
                     IRLWDKRRLIVPVGFFLEKPFQNWTRATSNIAAYITLWVDYSVRLDTIRTEFKKLCAT
                     SSLWDKELCQIVVVNSDNIAVELRLLMSAKTPQQCLELKYFV"
     gene            1071..1553
                     /locus_tag="PLY93_03300"
     CDS             1071..1553
                     /locus_tag="PLY93_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68534.1"
                     /translation="MISIKRTLFLFCTFSIILALSAQSKVNDIPVEDLSIQVKGVLES
                     YASILHRSASLEEAAEAFTPIAGGTLVNDDNTLSRNTIQFGLKKDFNNFKHYAYPIRI
                     TRVNRTLETSSGYGQTKVSGTGYKIWIAKKDPSKGMPAPVTIILPATGTAKVVNVGSY
                     "
     gene            1682..2752
                     /locus_tag="PLY93_03305"
     CDS             1682..2752
                     /locus_tag="PLY93_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRP68535.1"
                     /translation="MCTLTLWHSYNNEETRVFNALVADFEKAHPQIKIKAERVPFDGL
                     LPKLTSAAIAHRTPDIARVDIGHVARLAWGHVISPLNQLRAEETVGAMLPIAAKVASV
                     KTPRDKVAQLYAIPDQLTTVALYYNKKILAELQVTPPKTLGDLHRIGKIFRERGKSHK
                     ALGINASLWWAMPWLFLYNARVMNDTLDRCLLNSGESIAAIEALRTLYTEGTEGGAWL
                     SGAINPDQGFLTGRYAMILSGPWNLKTFQKIPFGVRLVPAHGKKISASNIGGSAMVVF
                     AASPRKSEAYQFLSYLVSEPVQKKWIQETGQLSVNARANQSLEHTFSPELKTFLRQLE
                     FAEPRPQLPGYDTLETIVAPYL"
     gene            3438..5693
                     /locus_tag="PLY93_03310"
     CDS             3438..5693
                     /locus_tag="PLY93_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRP68536.1"
                     /translation="MQKRSFLFLVFTALLYACNSEQRPRLYTPIIDLTDANGSIIDTA
                     CLSPLSTNTYKQPTAAEWPTIRDMKLGALYDGTSTTFRFFAPTAQQVDVVFYDTALQD
                     YSFPSAVIPMVRDGVGAVGLGVWSTAAQTTAVARPPNVAGAGKHYYRYRINCMKNYEN
                     KTINERDRYINDPYALASAGSHGHSIIIDTQWTVAAVFSGWNNGIDGVQGDIGGTNWF
                     GSARPNTARYIVYEAHVDDFTGSVSCLQSHSGSSCKTALQNYYSAEFALSTALGATSG
                     DNFSGKYTGFEAMSSYLKNTLGINAVHLMPLHESSNDNKDNPEGYSWGYLPSLFFAPE
                     SSYSTFCSRASASDACSYAEARAGYQVLELKRLIKKLHAQGIAVILDVVLNHTSNAFN
                     YLYQADPYGRYYFRTNGLNWTNIGTGNQLFDDRNQRPFAYKYILDNLKYWVKHYHVDG
                     FRFDLAVGTSEQTLRDIQNMVDDADVPGTWTTTVPPSATEASIRIDTEFLRSSAAETY
                     AADGQTRDNFNNTIKLPNFMTGENWLDAQRHGFMIQSDWASPNFASKRSSDYRGFSQW
                     NDYFRESVKKFIQGNADAQYTNRTKTAVYFSKGRDDGIWQGISKNPLDTLNYIESHDE
                     ETVARAVLDSKSKSAMGVTLLLTAHGIPMLYEGQEMMRNRERQNQSKSINNIDWQLVQ
                     TNGDMLALLLNCASPVLLCVTRTILPTQTPTLTAQRPTKRFLFLRKALCVQALLAPTR
                     DLSMASQSFVF"
     gene            5699..5923
                     /locus_tag="PLY93_03315"
     CDS             5699..5923
                     /locus_tag="PLY93_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68537.1"
                     /translation="MTGASQSFTVETGAASWRAVIASTGGNDNTNCGDPVSAVLRYAN
                     GTGANQISSWGTTNNTWSLPCYSAVVLVKQ"
     gene            6070..>7460
                     /locus_tag="PLY93_03320"
     CDS             6070..>7460
                     /locus_tag="PLY93_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRP68538.1"
                     /translation="MYDAEPHAPSFSVQQIKNYEVMGSKIVDGGVNFTLYSERATRVV
                     LLIFDDPESKYAVQEFVMSRFGNVWSVFVNGVGHGTYYGYRVWGPNFDYHPKWYPGTL
                     YGFKSDFDNQGNRFNPNKLLLDPYAKAVHRKHDWSKGMAASGPYRNVDTTSAAAKGIV
                     LKEDSYPWSVTEQAYWQRKQSTTNRHKRNALIFYEVHPKGISAAALAGIPGLAEDYQA
                     RFPGTFRGTGELAAYLANLGITAVEFLPIHEAGNDGGYWKYWTLNFFSPEVEYAYRPE
                     KGSPVNEFRRMVEEFHKYDIEVIIDVVYNHTGEGGLWREKVPLIEGNVPVNPNDPSNF
                     WAYDPAETATILSYRGIDNYGYYALTGPNKEFYYDHTGTGNMLRTANPAVRRMVIDSL
                     RYWVEEMHVDGFRFDLSEVLEKKDGQTTGNWNAYNYWKDWGGDAYSSTSATRRDMAAS
                     MWIINDPVLQKYDT"
BASE COUNT         1995 a         1865 c         1827 g         1773 t
ORIGIN      
        1 cacaaagtat ttgagctcta aacactgttg cggtgtcttt gcgctcatga gcaggcgtag
       61 ctccacagcg atattgtcgc tattgactac cacaatttgg cataattctt tatcccacaa
      121 agaggatgta gcgcagagtt ttttgaactc agtgcggatt gtatccaatc gcactgaata
      181 atcgacccac aaggttatat acgcggcaat gttcgatgtc gcgcgggtcc agttttgaaa
      241 aggcttttct aaaaaaaatc ccacagggac aataaggcgg cgtttatccc acaaacggat
      301 ggtgacgtac gtaagtgtaa tctcttccac aacaccccat tcgttttcaa ccgagacaac
      361 gtcgtcgtgg cgtatcggtt gagcgagcgc aatttgaatg ccggctagca ggttgcccaa
      421 accgcgctgc gcggtgactc ccaacaataa gcccacaacc cccgccgacg ccaaaagacc
      481 agtaccaatt tggcggaagc ttgtaaagct catgaggaca agccccgcgg ccgcaatgaa
      541 aataatcacc gaaaatacac ggcgcagcat gcggatttga gtgagcacac tacgtatatc
      601 tccaccctcc tgtgctttct gtttttgcaa aactattttt tccaaaatgc gaccagagcg
      661 cgcaatactc cacgcgatga gcgcaatcgt caacgctctc aaactctcaa ccgcaagttg
      721 gtggtgggct tgtgaaagtt tttgttgaat cgtaggcaag agcgcttggc ataaaatgac
      781 aggcaaaaga aatcgagtgg gatttcttaa ctcatcgaca aaaacatcgt cgatgtgcga
      841 acgagtacgc cctgcaataa atttgagaat tgcaaaaata gcaaagtgaa ttacgagagc
      901 gataagggta acaattgctg tttggaataa aatgttgtcg ttaatccacg ccatggtata
      961 ctgctatcag aatagtttaa aaaggaatgc aggctacaag ccttttttaa accaatgggg
     1021 tgtttttgaa gaagcttgac cgcgtttgta cgcattgata aaagtgtttt atgataagta
     1081 tcaaacgcac actatttcta ttttgcacct tcagcatcat tcttgctctt tctgcgcaga
     1141 gtaaagtcaa cgacatccct gttgaagatt tgtccatcca agtgaaaggc gtgcttgagt
     1201 cgtatgcaag tattttgcat cgcagcgcgt cgctcgaaga agcggcagaa gcatttacgc
     1261 caatcgcagg cggcactttg gttaatgatg acaatacgtt gtcgaggaac acaatccagt
     1321 ttggactcaa aaaggatttc aataacttta aacactacgc ataccctatc cgcatcactc
     1381 gcgtaaatcg gactctcgaa accagttctg gctatggcca aacaaaagtt tcgggaacag
     1441 gatacaaaat ttggattgcg aaaaaagatc catccaaagg gatgcctgcg cctgtaacca
     1501 tcatcttacc tgctacgggt acggcgaaag ttgtcaacgt cggtagttat tagcattatc
     1561 tatgccctgg ccattatatt tttcacggta ttccctgcgg ggggtaacta tcctgactta
     1621 tgtcacatgt tttttcgcgc tgctttcttg cacgcccaaa aatgacgcgc tgcaagatgg
     1681 cgtgtgcact ctcacgctct ggcattcgta taataacgaa gaaacgcgcg tgttcaacgc
     1741 acttgtcgcc gactttgaaa aagcgcaccc gcaaataaaa atcaaagccg aacgtgtgcc
     1801 gttcgatgga ctgttgccca aacttacctc ggcggcgatt gcgcacagaa cccctgacat
     1861 cgcccgcgtg gacatcggcc acgtcgcgcg cttggcgtgg gggcatgtta tctccccact
     1921 caatcagttg agggcagagg agacagtggg ggcgatgctg ccgattgcag ctaaagtggc
     1981 gagtgtaaaa acgccccgcg acaaggtggc gcaactctac gccatccccg atcaactcac
     2041 caccgttgcg ctttactata ataaaaaaat tttagccgag ttgcaagtaa cgccccccaa
     2101 gactctgggg gatttacacc gtatcggaaa aatttttcga gaacggggta aatcccacaa
     2161 agcgttggga ataaacgcat cgttgtggtg ggcgatgccg tggcttttcc tgtacaacgc
     2221 ccgcgtgatg aacgatacgc tcgaccgttg tttacttaat agcggtgaaa gtattgcagc
     2281 catcgaagcg ctgcgcacgc tttataccga gggcaccgaa gggggcgcgt ggctttcggg
     2341 ggcaatcaac cctgaccaag gttttttgac agggcggtat gcgatgattc tctccggtcc
     2401 gtggaatttg aagacgtttc aaaaaatccc ttttggcgtt cgacttgtac ccgcgcacgg
     2461 caaaaaaatt tctgccagca atatcggtgg ctcagcgatg gtggtgtttg cggcgtcgcc
     2521 gcgcaaaagc gaagcgtatc aatttctttc ttatttagtt tcagagccag tacaaaaaaa
     2581 atggatccaa gaaaccgggc agttgagcgt caacgcgcgc gcaaaccaaa gtctggagca
     2641 cacgttttcg cccgaactca aaaccttttt gcgccaactc gaatttgccg aaccgcgccc
     2701 acaactgcca ggctacgata cacttgaaac gattgttgca ccttacttgt aggctgcact
     2761 cgacggctcg ctttccgtcc gcgaggcgat gcaacgcgct tgccgcgatg tcgagagaga
     2821 tttattgttt tgagattggc gcataggctc ttaagtggat tacaatctaa gttggtataa
     2881 tcagactgtc tcgtattaac aatcaatcgt atgaagctaa aaccgaacag gcaaaggaaa
     2941 ggatatagaa tagtgtcatg caccccagac agctttacct ttgactgcca tgcagaggac
     3001 taaatggaaa agatttaata agccagcaca atatcaagat taacaatctg tagaggcaca
     3061 gcattcgttt gctttggata ttgtttaggg cttcccaagt tttttcactt ttgagaaaaa
     3121 agtaaaaatt ttcaaagaag ttatgatttt ttcgattaaa gcgctccttg ggatagttat
     3181 cccctaatgc gacataatcc agaaaattgg aatagttacc ccctaatgcg acataatcca
     3241 gaaaattggg atagttaccc cctaatgcga cataatccaa aaaattgagt tcgttacctc
     3301 cagatagctt aactgtaagt aaactatccg gcctcacaag ttcaaagcaa catccaaccc
     3361 atagaaatat gcaacccgca gaaaccaatt gacgtgtgca atggacaaag ccccatgtac
     3421 aaagcgtgca acataccatg caaaaacgaa gttttttatt cttagttttc accgcacttt
     3481 tgtatgcctg caatagcgag caacgaccac ggctttatac acccatcatc gatttaaccg
     3541 atgcgaatgg gagtatcatc gataccgcgt gcttgagtcc attgtcgacc aacacataca
     3601 agcaacccac cgccgccgaa tggcctacga tacgcgacat gaaactgggc gcactctacg
     3661 acggcacgag taccacgttc cgtttttttg caccgacggc gcagcaggtg gatgttgttt
     3721 tctacgacac ggcgttacaa gattattctt tcccctcggc ggttattccg atggtacgcg
     3781 atggcgtggg ggcagttggt ttgggtgttt ggagtactgc cgcacaaaca acggcggttg
     3841 caaggccgcc caacgttgcg ggggcaggga agcattatta ccgctaccgc atcaactgca
     3901 tgaagaacta cgagaataaa accatcaacg aacgcgatcg ttatatcaac gatccgtatg
     3961 cgctcgccag cgcgggctcg cacgggcata gcatcatcat cgacacccaa tggacggttg
     4021 ctgcggtctt tagtggatgg aataacggca tcgacggcgt ccaaggcgac atcggtggta
     4081 caaactggtt tgggagcgca cgccccaaca ccgctcggta catcgtgtac gaggcgcacg
     4141 tcgatgactt cactggttcc gtgtcgtgtt tgcaaagcca ctcgggctcc tcttgcaaaa
     4201 cggcattgca aaattattat agcgctgaat ttgctttatc cactgcgctc ggcgctacaa
     4261 gtggcgataa tttttctggg aaatacaccg gctttgaagc gatgtcttcc tatttaaaaa
     4321 atacgttggg gataaacgcg gtgcatctca tgccgttgca cgaatcttcg aacgacaata
     4381 aagacaatcc cgaaggttat agctgggggt atttgccgtc tcttttcttt gcacctgagt
     4441 cgtcgtactc gacgttttgc agccgtgcga gtgcgagcga tgcgtgtagt tatgccgaag
     4501 cgcgtgcggg ttatcaggtg cttgaattga aacgattaat aaagaagctt cacgcacaag
     4561 gcatcgctgt tattttagac gtggtgctca atcacacctc gaacgcgttt aattatctgt
     4621 atcaggccga tccctacggt cggtattact tccgtactaa tggcctcaac tggacgaaca
     4681 ttggtacagg taatcagctt ttcgacgacc gcaaccaacg ccccttcgcg tacaaatata
     4741 ttttggacaa cctcaaatac tgggtgaaac actaccacgt cgatggcttt cgtttcgatc
     4801 tcgcggtggg cacgagcgag cagaccttgc gcgatatcca aaacatggtg gacgacgcgg
     4861 atgtaccggg aacgtggacg accactgtcc ctccttcggc aaccgaggcg agtattcgca
     4921 tcgacactga atttttgcgt tcgagcgctg ctgaaactta tgcggctgat ggacaaacgc
     4981 gcgacaattt taataacacc atcaagcttc ccaacttcat gacgggagaa aattggttgg
     5041 atgcgcagcg gcacgggttt atgatccaga gcgattgggc atcccccaat tttgcaagta
     5101 agcgcagcag tgactaccga gggttttcgc aatggaacga ttattttcgt gaatctgtga
     5161 aaaaatttat ccaaggcaac gccgatgcac aatacaccaa ccgcactaaa accgcagttt
     5221 attttagcaa aggccgcgac gatggaatct ggcaaggcat ctctaaaaat ccactcgata
     5281 ctttaaacta tatagaaagc cacgacgaag aaaccgtcgc ccgcgcggtg ctcgattcta
     5341 aatccaaatc ggccatgggg gtaacgctcc tcctcaccgc gcacggcata ccgatgcttt
     5401 acgaagggca agagatgatg cgcaaccgcg aacggcaaaa ccaaagtaaa tccatcaaca
     5461 acatcgattg gcaactcgta cagaccaacg gcgatatgct tgcactcttg ttaaattgcg
     5521 catcgcctgt cctgctttgc gttacgcgga cgatcctacc aacgcaaact ccaactttga
     5581 cggcacaacg cccaaccaaa cgatttctct ttttacgaaa ggcgctctgt gtgcaggctt
     5641 tactggcgcc gacgcgggat ttatcaatgg caagtcagag tttcgtattt tagccaacat
     5701 gaccggcgct tcgcaaagct tcactgtcga aacgggcgct gcatcgtggc gcgcggttat
     5761 cgcatcgacg ggcggcaacg acaacaccaa ctgcggcgat ccagtcagcg cagtgttgcg
     5821 gtacgctaac ggtactgggg caaaccaaat tagcagttgg gggacgacaa acaatacgtg
     5881 gtctttgcca tgttacagtg cggtggtgtt ggtgaagcaa tgaaaaatat gagtaaaact
     5941 tctccacccg cccccctctc catgagggga tgtggcatca taacgatact aaaactaaaa
     6001 aaaatattat tcccaggcat agccacaata accctcgcct gcaccgggcc aaacgcgaac
     6061 ttctcgcgca tgtacgatgc cgagccgcac gcgccgtcgt tttcggtgca gcaaattaaa
     6121 aactacgaag tgatgggatc caaaattgtc gacggcggtg tgaacttcac tctttactcc
     6181 gaacgagcga cgcgggtggt actccttatt ttcgacgatc ccgaaagtaa atacgcggta
     6241 caagaatttg tgatgtcgcg ttttgggaat gtgtggagtg tttttgtaaa cggcgtgggt
     6301 catgggacgt attatggcta ccgcgtgtgg ggccccaatt ttgattacca tcccaaatgg
     6361 tatcctggaa cgctctatgg ttttaagtcc gactttgata atcaagggaa ccggtttaat
     6421 cccaataagt tgttgcttga cccatacgca aaagcagtac accgtaagca cgattggtcg
     6481 aaaggcatgg cagcttcggg gccataccgc aacgtcgaca ccaccagcgc tgccgcgaag
     6541 ggcatcgtcc tcaaggagga tagttatccc tggagcgtca ccgaacaggc gtactggcaa
     6601 cgcaagcaat cgacgaccaa tcgacacaaa cgcaacgcgc ttatttttta cgaggtgcat
     6661 cccaagggta tctcagcggc tgcgctcgcg ggcatccctg gccttgcaga ggactaccaa
     6721 gcgcgtttcc ctggcacgtt ccgtggcacc ggtgagttag cggcctatct cgccaactta
     6781 ggcattacgg cggttgagtt tctaccaatc cacgaagcgg gcaacgacgg tggctactgg
     6841 aaatactgga cgctcaattt tttctccccc gaggtggaat acgcgtaccg ccctgaaaaa
     6901 ggatcgccgg tgaacgagtt ccgccgcatg gtcgaagagt ttcataaata cgacatcgag
     6961 gtgattatcg acgtggtgta taaccacact ggcgaggggg gcttgtggcg tgagaaggtg
     7021 ccgctcatcg aaggtaatgt gccggtgaac cccaatgatc cttccaactt ttgggcatac
     7081 gatcctgcgg agaccgcgac catcctttca taccgcggca tcgataatta tggttactac
     7141 gcgctcactg gtcccaataa agagttttat tacgatcaca ccggcacggg taacatgctg
     7201 cgcaccgcga accctgcggt gcggcgcatg gtcattgata gcttacgcta ttgggtagaa
     7261 gaaatgcatg tcgatggatt tcgttttgac ttgagcgaag tcttagaaaa aaaggacggt
     7321 cagaccaccg gtaactggaa tgcgtataac tattggaaag actggggagg agatgcctat
     7381 tcctcgacga gcgctacgcg gcgcgacatg gctgcttcga tgtggatcat caacgatccg
     7441 gtgttgcaaa agtacgacac
//
LOCUS       DAOMPD010000062         7308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4368_length_7308_cov_2.625810, whole genome shotgun sequence.
ACCESSION   DAOMPD010000062 DAOMPD010000000
VERSION     DAOMPD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4368_length_7308_cov_2.625810"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..825
                     /locus_tag="PLY93_03325"
     CDS             43..825
                     /locus_tag="PLY93_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HRP68539.1"
                     /translation="MTLAISPCPNDAFAFYSLIQEGHYNVNFLDIEELNRALIAGRYP
                     IAKGSFAIIDNIAERYEILPTGAAIGKGVGPVFVGTLKNGARVALPGENTTAHRLFHF
                     WKKFNPELQITPVQMPFFEMLPSLESGQVDAAVLIHEGRFIYPAQGLDLICDLGAFWE
                     EKTGAMIPLGCIYAEKSLPTEVKSAFVASLQDSITKSQTAYSQKSTLYKEEILPYMRT
                     MAQEKDDHVVEAHVDTYVTEDTFELRSAAHKSIEVFREAIRP"
     gene            1007..1429
                     /locus_tag="PLY93_03330"
     CDS             1007..1429
                     /locus_tag="PLY93_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68540.1"
                     /translation="MVESQQLKNIEFLLGDIQKLLLHKGSQGSNTADVILSIVPMVAV
                     VFGSVLLFFLLLYNYRIRREKIRQQLPTESALDHLRFISLLAGCLSFSAGIPLTLFFV
                     FTNPANPGILGGLIPLSAGLGLIVFFYLSQKKKGAHDT"
     gene            1419..1928
                     /locus_tag="PLY93_03335"
     CDS             1419..1928
                     /locus_tag="PLY93_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP68541.1"
                     /translation="MTPDGGVFSKEDFDREFERALDMTYSIGLKLYRGREEDAADFTQ
                     SVYLFALKKRHKFEGRSAFSTWLYRLAWNFGLEDLRRKKRLLAEDIESFDIAAPESAE
                     PEFTEEETENLRRHIATLPDAYRLPLIMLFFENMTYSEISAITKIKEGTLKANVYRAK
                     NILRQKMGK"
     assembly_gap    2021..2030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2031..2275
                     /locus_tag="PLY93_03340"
     CDS             <2031..2275
                     /locus_tag="PLY93_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68542.1"
                     /translation="PLVKSWQFVSGVFLLLASPLSLYFFEAKLIYSPNVLLLMNVAAG
                     SIAFLLIFAGVAHRYSSPKNKAELLHKIETLRARLS"
     gene            complement(2323..2661)
                     /locus_tag="PLY93_03345"
     CDS             complement(2323..2661)
                     /locus_tag="PLY93_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP68543.1"
                     /translation="MVEVNKVADIPCARITASQLHMYFIPDLKDKLLKAISDEPNFLI
                     IDLSQVEYLDSSAMGVLFQVQKKMQHYGGALFLVGINSTIEIVFRLTKSDQHFTIYET
                     VEDVIKKINK"
     gene            2763..3584
                     /locus_tag="PLY93_03350"
     CDS             2763..3584
                     /locus_tag="PLY93_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRP68544.1"
                     /translation="MPLDLFSTVNTIIHEADLPRKRAALQELQQIWQNTPMRTIFFEQ
                     QDLSVEPVGNPSFAALCCIVHGSQVPRRRNLDTLEGRIIFLHALAHIEYSAIDLALDA
                     VYRFRNLPEKFYDDFLNVAFDEMRHFTMLEQLLSELSAHYGFLPVHTGIHDAMARSQH
                     SLRVRMAATHRHLEANGLDAHPELARKFMTFTDPFAEKVRAALGIIFEDEINHVRVGD
                     YWFRYACAKEGVSTDTFVADVESAIPGAKFGKKSLNIDARKRAGFTDRDIALLEN"
     gene            complement(3581..4252)
                     /gene="infC"
                     /locus_tag="PLY93_03355"
     CDS             complement(3581..4252)
                     /gene="infC"
                     /locus_tag="PLY93_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRP68545.1"
                     /translation="MQQRPSFPPKKPSTGGGGGQGRFNRFSKVRRPLREHRINEDITE
                     EKVRLIGEKGQEVLSTQEARARAQAQGVDLVEITKGQDVPVVRLVDYGKFKFEKAKKE
                     KEAKKKQKVIQIKEIKMGPKIDVGDFDRKAKEAINFLNEGDNVKVTMKFRGREMQHTA
                     LGAERLSEFYTKVAEAAHMDKKPNLEGRIMSMTLRSKGAKPKSASAQNNNPAPAAANA
                     VVANN"
     gene            complement(4314..5096)
                     /locus_tag="PLY93_03360"
     CDS             complement(4314..5096)
                     /locus_tag="PLY93_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRP68546.1"
                     /translation="MACIPAGDFIRGSNDYLDDEKPEEKVYISEFYIDKTEVTNKEFN
                     ECLAAGKCRECMKNGKCDYIGPRYGWRYKKDNQPVSGVSWFTAKEYCEFRGKRLPTEA
                     EWEKAARGPQGNIYPWGNEPATCKNAIIEEGKGDKVIKGCVEKHLEPVWHMPTAPVAS
                     RPAGVYGLYDMAGNVHEWVNDWYAKSYSACGDACRGKDPQGPCGGAEKCPGYTMRSVR
                     GGSWWWQAEHARGSKRRANVPGNTPSVDYHHFGFRCALTAPL"
     gene            complement(5242..5625)
                     /locus_tag="PLY93_03365"
     CDS             complement(5242..5625)
                     /locus_tag="PLY93_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRP68547.1"
                     /translation="MRDWQRQTKQREMLRNIFLESERPLTIQNLVALAHERHVKIGQA
                     TVYRTVNSLLEAGEIQAVQIPGGEAFYEKAHLHHHHHFKCQKCNKVYDLPGCPDGVDL
                     HKFLPKGFKLYSHDFTFYGVCKECG"
     gene            5708..6124
                     /gene="tsaE"
                     /locus_tag="PLY93_03370"
     CDS             5708..6124
                     /gene="tsaE"
                     /locus_tag="PLY93_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRP68548.1"
                     /translation="MKVVLENLDALKKYAQETAHEIHPPLLVLIHGNLGAGKTTLVSA
                     ILEAWGVESANSPTFNLRNDYYPQNFHAAHLDFYRLKNAESAWDILPFDEDFSQSVVF
                     VEWPEKVDAQLFTPFSRKLEIFIDVNSQGHRIIEKK"
     gene            6126..6773
                     /locus_tag="PLY93_03375"
     CDS             6126..6773
                     /locus_tag="PLY93_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68549.1"
                     /translation="MNSHLLIDTSTRYLVFARLGKEGGEKILTPVSLDAGDTAIDSAV
                     ATLFPSLDALDAIWLGLGPGSFVGLRSSFAYVRMLSMLSQKPVYTFFSSRLWRAFFNV
                     RESDWFLTRTNAKLFYAERYASEREAKSLELSAATSLVGNKFFWMDSWLSQAKSVEEK
                     TIIKEGKALYFADAQIKVSFLTQTLLQPQRVEEHTDLAPIYGHELNFTLAKPKLK"
     gene            6788..>7036
                     /locus_tag="PLY93_03380"
     CDS             6788..>7036
                     /locus_tag="PLY93_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68550.1"
                     /translation="MNKDSEKLLDRWIFHAYKSGNTVLPILPKEKNGVKTKSKDKKKQ
                     KKSPHQTSQHRDPFTTDAAQHLRANALVDNSTEQAQLKN"
     assembly_gap    7037..7046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7047..>7308
                     /locus_tag="PLY93_03385"
     CDS             <7047..>7308
                     /locus_tag="PLY93_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68551.1"
                     /translation="TGLLSVAKSGVAFVAIGKGVEAVVPASERGAALHRDKVEVVLTG
                     FNRGRFVAKVLKIVEPFSTEYLAQILGFTQSVATSHKKEKFA"
BASE COUNT         1950 a         1732 c         1606 g         2000 t
ORIGIN      
        1 catacgtgaa tacttggtgg cgtcgaaact ggcaaagctg cgatgacgct cgctatctca
       61 ccctgcccca acgacgcctt cgctttttat tcgctcatcc aagaagggca ttacaatgtt
      121 aattttctcg acatcgagga gttaaatcgt gcgctcatcg cgggcagata cccaatcgcc
      181 aaaggctctt ttgccattat agataacata gccgagcgct atgaaattct acccaccggc
      241 gccgccatcg gcaagggcgt agggccggta ttcgtcggca ccctcaaaaa cggcgcacgc
      301 gtcgcactcc cgggtgaaaa taccacggcg catcgtttat tccacttctg gaaaaaattt
      361 aatcccgagc tgcagataac gcctgtgcag atgccgtttt ttgaaatgct accatcgctc
      421 gaatcgggtc aagtcgacgc ggcggtgctc atccacgaag gccgctttat ctacccagcg
      481 caaggtcttg acctcatctg cgacctcggc gcgttctggg aagaaaaaac cggcgcgatg
      541 attcctttag gttgcattta cgccgaaaag agcctaccta ctgaagtcaa atctgcgttt
      601 gtcgcttcac ttcaagacag cattacaaaa agccaaacag cgtatagcca gaaatccacg
      661 ctatataaag aagaaatttt gccgtatatg cgtactatgg cacaagaaaa agacgaccat
      721 gtcgtcgagg cgcacgtcga tacgtacgta acagaggaca cattcgaact gcggagtgca
      781 gcgcataagt cgattgaagt ttttcgtgag gcaatccgac cgtaagttat aatcgatgcc
      841 aaactcgata gtttataagc gtacgatgct tgcgataaag cggaaaaaca atgcgcccac
      901 aaaatacttg tcagccccac ttaacgcata tctattgaga gagaagaata aatccttcgt
      961 aagaaaattg caaccgacgc gggaaaaaaa cgtctaagga gtaacgatgg tagagagtca
     1021 acaactcaag aatatcgaat ttttactggg ggacatccaa aaactgcttc tccataaagg
     1081 tagccaaggc tcgaacactg ccgatgttat tttatcgatt gtgccgatgg tcgcggtggt
     1141 tttcggttcg gtgttacttt tttttcttct cctctataat taccgtatcc gtcgcgaaaa
     1201 aatccgtcag cagctaccca ctgaaagtgc actcgaccac ttgcgtttca tcagcctttt
     1261 agcggggtgt ttaagttttt cggcaggcat cccgctgacc ctttttttcg tattcacaaa
     1321 tcctgcaaac cccggtattt taggcgggct tatcccgtta tcagcgggat tggggctcat
     1381 tgtttttttc tatctatcgc aaaagaagaa aggggcgcat gacacctgat ggcggcgttt
     1441 tttcaaaaga agatttcgac cgcgaatttg agagggcact cgacatgacc tactcgattg
     1501 gactaaaact ctaccgtggc cgcgaagaag acgccgccga tttcacacaa tcggtgtatc
     1561 tttttgcgct caaaaagcgg cacaagttcg agggacgatc cgccttttca acgtggcttt
     1621 accgtttagc gtggaatttt ggattagagg atttacgccg caagaaacgc cttctcgcag
     1681 aagatattga atcattcgac atcgccgcac cggaaagcgc tgaacccgaa ttcaccgaag
     1741 aagaaaccga aaacttacga cgccatatcg ccactttacc cgatgcatac cggctgccac
     1801 tcatcatgct tttttttgaa aacatgacct actctgaaat ttcagcgatt actaaaatta
     1861 aagaaggcac tctcaaagca aatgtctacc gcgcaaaaaa tatcttgaga caaaaaatgg
     1921 gaaagtgagg cattatgaaa gaacacatct acacgtacaa agaagcaatc cacactgctg
     1981 tcaatcgtga gtacacgcac aaagcccccg caacgttacg nnnnnnnnnn tgccgttagt
     2041 aaaatcgtgg caattcgtga gcggtgtttt tcttttgctg gcatcgccgc tcagtttata
     2101 tttttttgag gcaaaactta tttattctcc aaacgtgctt ttgctcatga acgtagctgc
     2161 cggttcgatt gcgttcctgc ttatttttgc aggggtggca caccgctatt ctagccccaa
     2221 aaacaaagcg gaacttttac acaaaattga aacgctacgc gcacgcctct cataaaaacg
     2281 gacgcgctat ctttgaccgt ttaacgatcc ttatttattt tttcatttat taattttttt
     2341 gattacgtct tctactgttt cataaattgt aaagtgctga tcggattttg taaggcggaa
     2401 cacaatttca atcgttgagt tgataccaac taaaaagagc gcaccgccat agtgttgcat
     2461 tttcttttgc acttggaaga gcactcccat tgcagaggaa tctaaatact cgacctgcga
     2521 taaatcaata attaaaaaat taggttcatc gctgattgcc ttcaaaagtt tatcttttaa
     2581 gtcgggtata aaatacatgt gtaactgcga tgcagtgatg cgcgcacagg gaatatctgc
     2641 cactttattt acttcaacca cggtgaaagt cttttagagc ggttgaacct gtcaatactt
     2701 tgaataccgc tataaagttg tcccgtggtt gcgaataaaa attttagtat attataatct
     2761 atatgccgct tgatttgttt tccacagtga acaccattat ccatgaggca gatttaccca
     2821 gaaaacgcgc tgcactgcaa gagttgcagc aaatttggca aaatacgcca atgcgtacga
     2881 ttttttttga gcaacaggat ttatctgtcg aaccagtggg aaatccctct tttgcggcat
     2941 tatgctgcat tgtgcacggt tcgcaagtac cgcgcagacg caacctcgac acactagaag
     3001 gacggattat ttttctacac gctcttgcgc atatcgaata ctcggctata gaccttgctc
     3061 tcgacgctgt ttatcgattc cgcaatttac ctgaaaaatt ttacgatgac tttctcaacg
     3121 tagcattcga tgagatgcgc cattttacga tgcttgaaca gttactcagc gaattgagcg
     3181 cccattatgg attccttcct gtgcacacgg gtatccacga tgcaatggcg cgttcgcagc
     3241 attcgctacg cgtgcgtatg gctgccacgc accgccacct ggaagccaac ggtctcgatg
     3301 cacaccccga gctcgcacgc aaatttatga cttttaccga tccctttgca gaaaaagtac
     3361 gcgctgctct gggtattatt tttgaagatg aaataaacca cgttcgtgtg ggcgactatt
     3421 ggtttcgtta tgcctgcgca aaagaaggtg tttcaaccga tactttcgta gctgatgttg
     3481 aaagcgcgat accaggggcc aaatttggca aaaaatcgct gaacatcgat gcgcgtaaac
     3541 gcgcaggctt taccgatcgc gatatagcat tgcttgaaaa ttaattattt gcgaccactg
     3601 cgttcgctgc tgccggcgct ggattattat tctgagcgga tgcagatttt ggtttggccc
     3661 ctttgctacg cagtgtcatg ctcatgatac ggccttcgag attaggtttt ttatccatgt
     3721 gtgcagcttc ggctactttg gtataaaact cagagagcct ttctgcgcct aaagcagtat
     3781 gctgcatttc acggccacga aatttcatcg ttactttaac gttgtcgcct tcgttcaaaa
     3841 aattaatcgc ttctttagct ttacgatcaa aatcaccgac gtcaattttt ggccccattt
     3901 tgatttcttt aatttggata actttttgtt tcttttttgc ttctttttct tttttggctt
     3961 tttcgaactt gaacttaccg taatcgacga gacgcaccac aggcacatct tggcctttgg
     4021 tgatttcaac aagatcgaca ccctgtgctt gcgcacgggc acgcgcttcc tgcgttgaaa
     4081 gaacctcttg gcccttttcg ccaataagac gtactttctc ttcggttata tcttcattga
     4141 ttcgatgttc gcgtagtggt cgccgaactt tcgagaaacg attgaaacgg ccttgaccgc
     4201 ccccaccacc ggttgatggt tttttaggag gaaaactagg cctttgttgc ataaatagtc
     4261 atgtttctgt aaacaggggc agttttatga aatggtcgcc gttgtcaatc acatcacaag
     4321 ggtgctgtga gcgcgcagcg gaaaccgaag tgatggtaat ccaccgaagg cgtgttgcca
     4381 ggaacattcg cccgtcgctt agagccacgt gcatgctcgg cttgccacca ccaagaacca
     4441 ccgcgtacac tgcgcatggt gtagccggga catttctccg cgccaccgca aggaccttgg
     4501 ggatctttac ctcgacacgc atcgccgcag gcagagtagc ttttcgcata ccagtcgttt
     4561 acccattcgt gtacgttacc tgccatgtcg tagaggccat aaacgcccgc aggacgcgag
     4621 gcgacgggtg cagttggcat gtgccacaca ggctcaagat gtttttcaac acaacctttt
     4681 atgactttgt cccctttccc ttcttcgatg atggcatttt tacaagtagc aggttcgttg
     4741 ccccatgggt aaatattccc ctgtgggccc cttgctgctt tttcccattc ggcttcggtg
     4801 ggtaatcttt taccacgaaa ttcacaatat tctttcgctg taaaccagct cactcccgac
     4861 accggttggt tatctttttt ataacgccaa ccatagcgtg gaccgatata atcgcacttg
     4921 ccatttttca tacactcgcg gcatttaccc gccgccaagc attcattaaa ttctttatta
     4981 gtaacctcag ttttatcgat atagaattca ctgatatata ctttctcttc gggcttttca
     5041 tcgtctaaat aatcgttcga acctcggatg aaatctcccg cagggataca ggccatgccg
     5101 gaaatttctt ttgagcaaga gataatattt tttttacacg cggcaaatag actaactatg
     5161 acaataaaaa gagaatagaa ataaattttg tacatgggaa atttttgcag aggattttat
     5221 gcggtaaacc gatatattga tttatccgca ctccttacat acgccgtaaa aagtaaaatc
     5281 gtgcgaataa agtttgaaac ctttgggcag aaatttatgc aaatcaacgc catcggggca
     5341 gccgggtaaa tcgtagactt tgttacactt ttgacatttg aagtgatggt ggtggtgtag
     5401 atgcgctttt tcgtaaaagg cttcgccgcc gggtatttgt accgcttgaa tttcacctgc
     5461 ttcgagaagc gaattcacag tgcggtacac agtggcttgg ccaattttaa catgccgctc
     5521 atgcgcgagt gcgaccaaat tttgaatcgt tagaggtctt tcgctttcca aaaaaatgtt
     5581 acgcagcatt tcacgctgct tcgtttggcg ttgccaatcg cgcacaatgg gatataagat
     5641 agatgggaca cagcctcgca acgcataatc tactttcccg cctgtcttct ttttcgaact
     5701 ataaaaggtg aaggtcgttt tagaaaattt ggacgcatta aaaaaatacg cgcaagaaac
     5761 cgcacacgaa atccatcctc cacttttggt gctcatccat gggaacttag gcgcaggcaa
     5821 aacgacgtta gtgagtgcaa ttttggaggc gtggggtgtg gagagcgcca attcacccac
     5881 atttaatttg cgtaacgatt attatccgca aaacttccat gctgcgcatt tagattttta
     5941 tcggttgaaa aatgcagaga gcgcatggga tatactcccc tttgacgaag atttttcaca
     6001 gagtgttgtt tttgtcgagt ggccagaaaa agtggacgca cagttgttta cgccgtttag
     6061 tcgaaagtta gagattttca tcgacgtcaa ctcccaaggc cacaggatta tagaaaaaaa
     6121 ataaaatgaa ttcccactta ctcatcgata cctcgacgcg ctatcttgtg tttgcacgct
     6181 taggcaaaga aggcggagaa aaaatattaa ccccggtgtc tctcgatgcg ggggacaccg
     6241 ccatcgacag cgctgtcgct acgctctttc cttcgctcga tgcgttagac gccatttggt
     6301 tgggcttagg cccaggctcc tttgtcggat tgcgctcgtc gtttgcgtat gtgcgtatgc
     6361 tttcaatgct ttcgcaaaaa cctgtttata catttttttc gtcgcgccta tggcgtgctt
     6421 tttttaacgt gcgcgaaagc gattggtttt taacgcgcac taatgcaaaa cttttttacg
     6481 ccgaaaggta tgcctccgaa cgcgaagcga aatcgcttga actgagcgct gcgaccagcc
     6541 ttgtcgggaa taaatttttt tggatggata gctggctttc gcaagcgaaa tccgtagaag
     6601 aaaaaacaat tataaaagaa ggaaaggccc tctattttgc cgatgctcaa atcaaggtat
     6661 ctttcctcac gcaaacgctt ttgcaaccac agcgcgttga agaacacacc gatttagcgc
     6721 cgatctatgg gcatgagctc aattttacct tagccaaacc aaaacttaaa tagaatgtac
     6781 tacatatatg aataaagatt ctgaaaaact cctcgaccgt tggattttcc acgcgtataa
     6841 atcggggaac accgtgttgc ccattttgcc caaagaaaaa aatggagtaa aaacaaagag
     6901 taaagataaa aagaaacaaa aaaaatctcc acaccaaacg tcgcagcata gagacccatt
     6961 taccaccgat gcagcgcagc acttgcgcgc caacgcgctc gtcgataata gcacagaaca
     7021 ggcgcaactt aaaaatnnnn nnnnnnacac tggcttgttg agcgttgcga agtcaggggt
     7081 ggcgtttgta gccatcggca aaggcgtcga ggcggtggtg cctgccagtg aacgcggcgc
     7141 ggcgctgcac cgcgacaaag tagaggtagt gctcaccggt tttaaccgcg ggcgttttgt
     7201 ggcaaaggta ttaaaaattg tcgaaccttt ttcgacagaa taccttgcac aaattttagg
     7261 atttacccaa agcgtggcga cgtcgcataa gaaagaaaaa tttgcttt
//
LOCUS       DAOMPD010000063         7181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4460_length_7181_cov_3.202498, whole genome shotgun sequence.
ACCESSION   DAOMPD010000063 DAOMPD010000000
VERSION     DAOMPD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_7181_cov_3.202498"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLY93_03390"
     CDS             complement(<1..979)
                     /locus_tag="PLY93_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68552.1"
                     /translation="MVKHVDIQERTMRIFISIVCTFGLLTPSISAEVYPLQLFRSYGD
                     VTPQAALLIGKINTKGTDNSIPPEQILGYDARMAVVSASIDNAAKVYPGMKVYIVKRE
                     RNHTENRAALIVAEGEIDAITNTVFQGRVARIRGQFSMVSRQHFIAIPQNATNRDGNT
                     RSAHEYLLEAERYRHERDLARAAQMLEHARALEPTNPLVELRYAELALTNDAHEKAHR
                     ALAQAFRERRRLENVNDYLKLGALYLTLEMNAMPSLNQEILKKGTSLLMQMRAFEKDL
                     GQYGRDLTPPKSRRMRVKQTHFSAVYHYQYGRLLNMLAITLREYSPESIT"
     gene            complement(1091..1585)
                     /locus_tag="PLY93_03395"
     CDS             complement(1091..1585)
                     /locus_tag="PLY93_03395"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRP68553.1"
                     /translation="MDRQKIVRTFLRYTLYIIGFFILSAFANWLQAPKPSANTVVSLP
                     LSTLDGTQKILPTAQGKTTVVYFFAPWCTVCKLSMDAMNMFEGSARVQALTIGLDYEN
                     RNELAAFQERMKAPIYAGSQELQRQFQVDRYPTVYIIDGNGKIKHTMVGYASRLGIWI
                     RSWI"
     gene            1789..2691
                     /locus_tag="PLY93_03400"
     CDS             1789..2691
                     /locus_tag="PLY93_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68554.1"
                     /translation="MAKINFAGLSGADLADANELDAKYTTLARAISTYYGEGMAFASH
                     LSAPNARDNLGAFPSIYFGIGFGSTFAKTNAIKDSVDSSIKDIVPGILPAPNISINFG
                     FGINRSWDVRFSFFPNVPVSLGSSDFPVIKFGTYRARVGYHVKEGGFLKPGLTIAGFV
                     SYTTGSISYVENGQSVTSGPTSLANMSSTLSSSWQYLGVGPEARFWYDLKFFNPFVGY
                     SLGLQIGQMTTGLDINGDLTVSGTPYGTGSINISERKAARIITHRILFGFEISLFVID
                     IGVEGQVDLTSGLVGIAAGTAFRF"
     gene            complement(2681..3286)
                     /locus_tag="PLY93_03405"
     CDS             complement(2681..3286)
                     /locus_tag="PLY93_03405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP68555.1"
                     /translation="MQAADDFIQYQQFLISDQREKLIPSAVYTSALTKDVRENLNVLD
                     FGCGHGYVAVLLAHLPLKGLHVYACDCDEECLDTLWGRIAHRDIKNLTAFHLSNYAQI
                     YTPTWLPPIDHVFFSFSVSALEHPDISLPQIVKQMPAGTQFHFFDWNPHKSHPQVDIF
                     VPPARRIQIDDFKKLLEYSGLTLQHEESGKQIYYTMRAIKT"
     gene            complement(3298..3600)
                     /gene="gatC"
                     /locus_tag="PLY93_03410"
     CDS             complement(3298..3600)
                     /gene="gatC"
                     /locus_tag="PLY93_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRP68556.1"
                     /translation="MADALSITEVQKIAGLSKLRLEGDELQKMAHDFNGILNFVAQIK
                     DANTENTPALDHPLGLINSERSDTPVPSIEQEAIRAFAPKFEAGFFVVPRVIETEH"
     gene            3748..4131
                     /locus_tag="PLY93_03415"
     CDS             3748..4131
                     /locus_tag="PLY93_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRP68557.1"
                     /translation="MNYGFSKTVLGSFDEVEARVREALQKEGFGVLTEINVQATLKKK
                     LDVDFRPYKILGACNPSFAHKALSAEAHIGLMLPCNVVVQQGDKGIEVSAIDPIASMM
                     AVKNDALGAIASEVREKMRRVVETL"
     gene            complement(4128..5270)
                     /locus_tag="PLY93_03420"
     CDS             complement(4128..5270)
                     /locus_tag="PLY93_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68558.1"
                     /translation="MIYTSALTAHQLEFESGLLKNAITPSADYGSVVYKHEEYRVGMF
                     TAPTSKGLIAAYTSENSPWLLDVGQREKSLAQGAVVGNGAYLPWSNSFTFKEPIGIRT
                     GYNARYIGVNAAYYQRDSQNVAFPELTITPTNWLSMSGGAVEESMGVGTAFPIFSLNV
                     GKRGAWDGFHAALTAVGKENQFFYAAYKGNASVRLLAFHSESPGALASGIYAQNRGVA
                     AQFFSTHWFAQFFQTQSQFGMLRYTGEYLTAIYTKEEKSSLVGVSLRHNPTGFHARAG
                     ATFGIDGSLQSLAGLGYADIIFVGGGSYTLYSKQSLEPIIFPAEWYSSVLLQSTSMRI
                     QDHGIKMMALVNSEVVQGFVAVTHSTDAYDRDQFNFFMRVAGRVVF"
     gene            complement(5297..6202)
                     /locus_tag="PLY93_03425"
     CDS             complement(5297..6202)
                     /locus_tag="PLY93_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68559.1"
                     /translation="MFHFLLYRIGAVFLFCLTTNHLFADLSAQLTHQSVDAHTSSQTL
                     EIEKQHENYAFSFLGQRDTLPNAQRFTNAALIASSRKNFNEHQLSLGLGAHFYQQQVR
                     NTAAQTPNFVPAFALEWRHPRFLGSVQVSQKMTHAAIALLADLFVPVEWSSSFLSLPA
                     QPYRWSLDAYASLFAWGGIVLGYEPITAFSRAGFFVTPSATLQARALVRMRQNGEAFA
                     EFSLGYVFEEKSHTPLVEPTKTHAAQEKIPLRKKERVVPRFSVLVKWGLTPVEALRLS
                     REKNICALSINAQALLKRHKWECRA"
     gene            complement(6391..7017)
                     /locus_tag="PLY93_03430"
     CDS             complement(6391..7017)
                     /locus_tag="PLY93_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HRP68560.1"
                     /translation="MEFWISTTRRAEFVFTVSLLLVVMLSMLRYLHYNETRIGVQVAD
                     PLLTVLPAIDLSELIFFLLYAGLFSTVFLLLPQPHRLLIGLQVYIFYAALRTLSMWLL
                     PLEAPAGLIPLTDPLMSWASTGVQLNKDLFFSGHTSTMFIFYLLLPPGRIKSVYLIGF
                     IVMILSLIIQRVHYSVDVLAAPFFSYGALGIVLHLRKKLNLDADLHDG"
BASE COUNT         1887 a         1617 c         1761 g         1916 t
ORIGIN      
        1 ttgtaatgct ctctggcgag tattcgcgca gcgttatcgc cagcatgttc aatagtcgtc
       61 cgtattggta gtggtatacc gccgaaaagt gcgtttgttt cacgcgcata cggcgtgatt
      121 tcggtggcgt taagtcgcgc ccatattgac ctaaatcttt ttcaaacgct ctcatttgca
      181 tcaagaggga agtccctttt ttgaggattt cttggttgag cgacggcatg gcgttcattt
      241 caagagttaa atacaacgca cccagtttta agtagtcgtt cacattttcg agacgccgcc
      301 gttcacggaa tgcttgcgcg agtgcgcggt gcgctttttc gtgtgcgtcg tttgtcaatg
      361 caagttcggc gtaacgcaac tcaacaagtg gatttgtcgg ttcaagcgca cgcgcgtgct
      421 ccaacatctg tgctgcccgc gctaaatcgc gttcgtggcg gtagcgttct gcttccagta
      481 ggtattcgtg cgcgctccgt gtgttgccgt cgcggttggt tgcgttttgt ggaatcgcaa
      541 taaaatgttg gcggctcacc atcgagaact gacctcggat acgagcgacg cgaccttgga
      601 agacagtgtt cgtaatagcg tcgatttctc cctcggcaac aatgagcgcc gcgcggtttt
      661 cagtgtggtt gcgttcgcgc ttcacaatat aaactttcat gcccggataa actttcgccg
      721 cgttgtcgat ggatgcgctc actactgcca tgcgcgcgtc gtagcccaaa atttgttcgg
      781 gtggaattga gttgtctgtg cctttagtat taatttttcc aatcaggagc gccgcttgcg
      841 gcgttacgtc gccgtaacta cgaaaaagtt gcagcggata cacttcggca ctgatggatg
      901 gcgtaagtag gccaaacgtg catactattg atataaaaat tcgcatcgtt ctttcttgaa
      961 tatcgacgtg ttttaccaaa aatttttaca tattttatga atgtgggcgg tatctcgcac
     1021 agttttgagg cattatctat tatggagtat agggggtaac tatccaagcc ggcaattctt
     1081 cacaaaaaaa ctagatccac gatctaatcc agatacccaa tcggctcgcg tacccgacca
     1141 tcgtgtgttt tatttttccg ttgccgtcga tgatgtaaac cgtggggtat cgatcgactt
     1201 ggaattggcg ttgcaactct tgcgaaccgg cgtagatggg cgctttcata cgttcttgaa
     1261 atgctgctaa ttcgttgcga ttttcatagt cgagcccgat cgtcagtgct tgcacacgcg
     1321 cgctgccctc gaacatattc atggcgtcca tggacaactt acagacagtg caccacggcg
     1381 caaaaaaata cacgactgtg gtttttcctt gagcagtggg aagtatcttt tgggtgccat
     1441 cgagcgtaga aaggggtagt gacaccacgg tatttgcgga gggtttggga gcttgcagcc
     1501 aatttgcaaa tgcactcagt ataaagaaac caatgatgta aagtgtataa cgcaaaaacg
     1561 tacgtacgat cttttgtcga tccatacaga gaatatacaa aaaaaataaa tattatcaaa
     1621 ctttagggtg tatatggtag ttcgtgaaaa ttaaaggaga tcgcgtacac ttctcttgag
     1681 tataccgcat ttcatgtcga tattcgtaag gtagagtcgt ctaatatgaa gatgtttcag
     1741 cgaatcgtta tttttacatt tacgctgtgt ttggccacaa caccgattat ggcaaaaatc
     1801 aattttgcag ggttgagcgg cgccgacctt gccgatgcca acgaactcga tgcaaaatac
     1861 acgactttag cgcgggctat ctccacgtac tacggcgaag gtatggcgtt tgcatcgcac
     1921 ctttcagctc cgaatgcacg cgataatttg ggagctttcc cgtcgattta ttttggtatt
     1981 ggttttggaa gcacttttgc taaaacgaat gccattaaag atagtgtcga ttcgtcgatt
     2041 aaagatatag tacccgggat tctgcccgcc cccaacatat ccatcaattt tggtttcggc
     2101 atcaaccgta gctgggatgt gcgtttctcg ttttttccca atgtgccggt atctttaggt
     2161 agcagcgatt ttcctgttat taagttcggc acataccgag cacgggtcgg ctaccatgtc
     2221 aaagaaggcg ggttcttaaa accaggcctt accatcgcag ggtttgtttc gtacaccaca
     2281 gggagtattt cttatgtaga gaatggtcaa agcgtcacct ctggcccaac gtcgctcgct
     2341 aatatgtcgt cgacgctctc atcgtcgtgg cagtatttgg gcgttggtcc cgaagcacgg
     2401 ttttggtatg atcttaaatt ttttaacccg ttcgtgggct attcgctcgg tctacaaatt
     2461 ggccaaatga caacagggct cgacatcaac ggtgatttaa cagtaagtgg cacgccttat
     2521 ggaactggca gtatcaacat ttctgaaaga aaggccgcgc gcattattac gcaccgcatt
     2581 ctttttggtt ttgaaatttc gctttttgtt atcgacattg gtgtcgaagg ccaagtcgat
     2641 ttaaccagtg ggcttgtggg tatcgctgcc ggcactgcat ttaggtttta atcgcacgca
     2701 ttgtataata aatttgcttt ccgctttctt cgtgttggag cgtcaatccg ctatattcta
     2761 ataatttttt aaaatcatcg atttgaatgc gccgcgccgg ggggacaaaa atgtcgacct
     2821 gtggatgcga tttatggggg ttccaatcga agaaatggaa ttgagtgccc gcaggcattt
     2881 gttttacaat ttgcggtagg ctaatatctg gatgttccaa cgcagaaacg ctaaaagaaa
     2941 aaaagacgtg atctatcggc ggtaaccaag tgggtgtata gatttgcgca taatttgaaa
     3001 gatgaaatgc tgtgagattc tttatgtctc gatgtgcaat acggccccag agtgtatcca
     3061 aacattcttc atcgcaatcg caggcgtaaa cgtgcagccc tttaagagga aggtgcgcca
     3121 gcaaaaccgc tacgtaaccg tgaccgcaac cgaagtcgag cacgttcaag ttttcgcgca
     3181 cgtcctttgt cagtgcagac gtatacacgg cggaggggat taatttttct ctctgatcgg
     3241 agatcagaaa ttgttggtat tgaataaagt cgtcggcggc ttgcaatgcg tgtttattta
     3301 atgttctgtc tcaataacgc gtggtacgac aaaaaagccc gcttcaaatt ttggggcaaa
     3361 cgcgcggatg gcttcctgtt caattgatgg cacaggagtg tcgctccgtt cgctgttaat
     3421 cagtcctaat gggtgatcta acgctggagt attttctgta ttagcgtctt taatttgtgc
     3481 aacgaaattc aatatgccgt taaaatcgtg cgccattttt tgtaactcgt cgccttcaag
     3541 gcgcaactta cttaaccctg caattttttg tacttcggtt atactcaacg catctgccat
     3601 ggtagaaaat agtaagtgag gataaggtgt atagcaagaa ctggtataga ccggtgaaag
     3661 acaaatacca ccgaatcgca tagttcgtag ataagctcta aatcaataaa attcgctgat
     3721 gtacaagttt ttgtgcataa tatgtgcatg aactatggat ttagtaaaac cgttttgggc
     3781 tcattcgacg aggtggaagc gcgtgtgcgt gaagcgttac aaaaagaagg ctttggtgtt
     3841 ttaacagaaa tcaacgtaca agccacactc aagaaaaagc tcgatgtgga ttttcgcccg
     3901 tataaaattt taggcgcgtg caacccatcg ttcgcgcata aagcgttgag cgccgaagcg
     3961 cacatcggcc tcatgttgcc gtgcaatgtg gttgtgcagc aaggcgacaa aggcatcgaa
     4021 gtatcggcga ttgaccctat tgcatcgatg atggcagtta aaaatgacgc actcggtgcg
     4081 attgccagcg aagtacgtga aaaaatgcgc cgcgtggtgg aaactctcta aaaaacaaca
     4141 cgaccagcca cgcgcataaa aaaattgaat tggtctcggt catacgcgtc tgtgctgtgc
     4201 gtgacggcga caaaaccttg tacgacttcg ctattcacaa gcgccatcat tttaatacca
     4261 tggtcttgga tgcgcatgct cgtcgattgc aaaagcactg acgaatacca ctctgcggga
     4321 aaaataatcg gttctaaact ttgtttactg tagagtgtgt agctcccacc cccaacaaaa
     4381 ataatatccg catagccgag tcccgctaag ctttgcagtg aaccgtcgat accgaatgtt
     4441 gcgccggcgc gtgcgtggaa tcctgtgggg ttgtgtcgga gtgaaacccc caccaacgat
     4501 gatttttctt ccttagtata aatcgctgtc aagtattcac cggtgtagcg gagcataccg
     4561 aattggcttt gtgtttgaaa aaattgagcg aaccagtgcg tggaaaaaaa ttgtgccgcc
     4621 acgcctctgt tttgcgcgta gatgccgctg gcgagcgcac ccggactttc actatggaac
     4681 gctaaaaggc gaacacttgc atttcctttg taagcggcgt aaaagaattg gttttctttc
     4741 ccgacagccg tcaatgccgc atggaatcca tcccatgcac cgcgcttacc cacgttgagt
     4801 gaaaaaatag gaaaagcggt tcccaccccc atgctttctt ccacggcgcc gcccgacata
     4861 ctcaaccaat tggtgggagt gatggtgagt tctgggaacg ccacattttg cgagtcgcgt
     4921 tggtagtatg cggcgttcac gccgatgtaa cgcgcgttat accctgtgcg gatgccgatg
     4981 ggctctttga acgtaaagct atttgaccac ggcaggtacg cgccgttccc aacgaccgcc
     5041 ccttgagcga gcgatttctc gcgttgtcca acatcgagca gccacggtga attttcggac
     5101 gtgtacgcag cgatgagacc cttgcttgtt ggcgcggtga acatcccaac acgatattct
     5161 tcgtgcttat aaacaacgct gccataatcg gcagaagggg tgattgcatt tttgagcaac
     5221 cccgattcaa actccaattg atgtgcggta agtgcacttg tgtaaatcaa tagtgttata
     5281 aaacatataa aaattttcat gcgcggcact cccacttgtg gcgtttgagg agcgcttgtg
     5341 catttatgct caacgcgcag atattctttt cacgcgataa acgcagcgcc tcaacggggg
     5401 tgagtcccca ttttacaagt actgaaaagc gtggtacaac acgctctttt tttcgcagtg
     5461 ggattttctc ttgtgccgca tgggtttttg ttggctcaac aaggggcgtg tggctttttt
     5521 cttcaaaaac atatcccagt gaaaattcgg cgaaagcttc gccgttttgt cgcatacgca
     5581 ccagcgcgcg tgcttgaagc gtcgctgaag gcgtgacaaa aaatcccgcg cgcgaaaaag
     5641 cggtgatggg ttcatacccc aaaacaatcc caccccacgc gaagagcgat gcgtacgcgt
     5701 cgagcgacca acggtacggt tgtgcgggca gcgataaaaa actactgctc cattccacag
     5761 gaacgaataa atctgccagc aaggcgatcg ccgcgtgcgt cattttttgc gaaacctgaa
     5821 cgctaccgag aaagcgaggg tggcgccatt ccagcgcaaa cgcaggcacg aaattgggag
     5881 tttgtgcggc ggtatttcgt acttgttgtt gataaaaatg cgcacccagg ccgagcgata
     5941 attggtgttc gttaaaattt tttctcgaag aagcaatcag tgccgcgttt gtaaaacgtt
     6001 gtgcattcgg caacgtatcg cgctgtccca aaaacgaaaa cgcatagttt tcgtgctgct
     6061 tttcgatttc cagagtttgg cttgaggtgt gtgcatcgac actttgatgc gtgagctgcg
     6121 cactgaggtc agcgaaaagg tggttggtag taaggcaaaa aagaaatacc gccccaatgc
     6181 ggtacaataa aaaatgaaac atggaaactc cacacgtatt ggttggaggg ctgactcatt
     6241 ggctgctggt gcaggtaatg aaccaccctt ctatatatta aataccagaa aacggtcgaa
     6301 ttttctcact tttgaagaaa aaagtgcaat tttttcaaaa aaacatcagt ttttttgtta
     6361 aaaagcccct ttaactaggg aagtagtagt ctacccatcg tgtaaatcag cgtctagatt
     6421 gagttttttg cgaaggtgaa gtacaatgcc gagagcgcca tagctgaaaa aaggcgccgc
     6481 gagtacatcc acggaatagt gcacccgctg aataatcagg cttaggatca tcacaataaa
     6541 accaatgagg taaactgatt ttatgcggcc aggcggtagt agtaaataaa aaataaacat
     6601 cgttgaggtg tgtccagaaa aaaataaatc tttgttgagt tgcacgccgg tgctcgccca
     6661 cgacatcaaa ggatcggtca gcggaatcaa tcctgccggt gcttcaagtg gcaagagcca
     6721 catcgaaagg gtacgcaatg ccgcgtagaa aatatatact tgaaggccaa tcaataaccg
     6781 gtgtggttgc ggtaagagta aaaataccgt tgagaaaagc cccgcataga gtaaaaagaa
     6841 aatgagctcg cttaaatcaa tcgctggtag aaccgttaac agcggatcgg caacttgaac
     6901 gccaatgcgt gtttcattgt agtgcaaata acgcagcatt gagagcatca ccaccaacaa
     6961 taacgacacg gtaaaaacaa actcagcgcg gcgcgtagtg ctaatccaaa actccatcca
     7021 attgcgtcga acacgctcag tataactctg ctttttcaaa cgaacactcc cgaaatttac
     7081 acagatgata gttttacgaa ggccgtcaaa cataaaaaag aagaagtata tgcaacccac
     7141 agagataact gacacttttg gggtcaagtt gagtgcgttt t
//
LOCUS       DAOMPD010000064         7124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4506_length_7124_cov_3.158580, whole genome shotgun sequence.
ACCESSION   DAOMPD010000064 DAOMPD010000000
VERSION     DAOMPD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4506_length_7124_cov_3.158580"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLY93_03435"
     CDS             complement(<1..206)
                     /locus_tag="PLY93_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP68561.1"
                     /translation="MISLQNAGVSFGARDLFEKVSIQLNEGVRYGITGANGSGKSTLL
                     KVLAGREHLSEGKVDIQKGVRIAY"
     gene            complement(203..1414)
                     /locus_tag="PLY93_03440"
     CDS             complement(203..1414)
                     /locus_tag="PLY93_03440"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRP68562.1"
                     /translation="MALGIGKSIVLVLDGFGVGEQPDAYLYGKRGANTLAHIAEMRAP
                     LKIHNLVRMGISNLTYLKGHPREEEPIGFYGKITQRNEGNDSIAGHRELLGTYQEDKY
                     LTYPNGLPSEIMAELVKAVGKPFLGNANDVQQGFLTKYGDIHLETGNPILLVTDDSLI
                     HIYVHLDKFQPDEFYLLGHNAWEVLSKYGLGRLHVHYFVGDAAGFRIDDSSPKAMFQS
                     PPQQPTLAVYAHNAGIPVHTIGKVSEMIEGAAITETKDVKSNAECLEVLHEIIREGEV
                     NRYKQNIILATLPDLDALYGHNRDVIGYIDALEAFDQYLPRLFRAMENEDLLYIVSDH
                     GNDPTAEDTCHTREYVPLLVYSRMFRPRRQANLGIRLTLADIAETIADSYDLNVHFAA
                     SSFWENMISQL"
     gene            1607..1909
                     /locus_tag="PLY93_03445"
     CDS             1607..1909
                     /locus_tag="PLY93_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68563.1"
                     /translation="MKSFTVTGDTPEKFMRKVERALYKGELGKFSKMVLAMPDAKIAF
                     EKMGVTEFSFKISQDGETFTAQKVDEKVAVTHIFFRNEVERELERIIARFGGTIKK"
     gene            1947..2363
                     /locus_tag="PLY93_03450"
     CDS             1947..2363
                     /locus_tag="PLY93_03450"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HRP68564.1"
                     /translation="MNIQEDLKKYIDKASSQIDIGKEKLEGLVASLALDEKKAHLDGI
                     IQSLKDKGFKETVASWIGSGENQAIDPEKIKEALGVHKIEELAAQAKMKASEVPQALS
                     SLLPQIIDKLSPEGKEPESGLAATAVKFLKGIQAKV"
     gene            complement(2481..3530)
                     /locus_tag="PLY93_03455"
     CDS             complement(2481..3530)
                     /locus_tag="PLY93_03455"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRP68565.1"
                     /translation="MKILVINCGSATIKADVFTQEKGELQFSGKSLAENFSDYKEAIL
                     GIVADFERRGVLKAEELTAIGHRVVHGGLKFFEPVIIDDSVIQTIREYCIFAPLHNPA
                     NLAGIEYSMERFNVPQVAVFDTAFHQTMPPVATTYAIPPRLSQKYNIRRYGFHGPSHE
                     YVTRECAKQMNIPYNEFNAISLHLGNGASICCVRNGKSIDTSMGFTPLEGLVMGSRSG
                     DIDAGLLLFLQDAEKLTPKEMDTLLNKESGLKGLCGTNDMREIEKRATEGDASALLAL
                     QIFVERVRKYLGAYLMHGKPHAIIFTAGIGENSKIIREKICAGLEHLNLPPVVVVETD
                     EEKVIAEAALKLTQS"
     gene            complement(3527..>3937)
                     /locus_tag="PLY93_03460"
     CDS             complement(3527..>3937)
                     /locus_tag="PLY93_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRP68566.1"
                     /translation="SAVEVVTPKIVSTMDAAALAKMSDRNQFSGGIVDGPFALDNAIN
                     PAAAKYKKIGGPVAGQADILLVPDIVSGNALAKTFAHITGGTAAGVIFGAKVPVILPS
                     RADSKASKFAGIMSAVYAAGVNIKKRVKIGKVHF"
     assembly_gap    3938..3989
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3990..4478)
                     /locus_tag="PLY93_03465"
     CDS             complement(<3990..4478)
                     /locus_tag="PLY93_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRP68567.1"
                     /translation="MIINFAQVEKKIFEYPMLRIAVAAGNDSETLYAVTEARDKHIAE
                     TVIVGDEEKIHTVAAQEKLDLRGIEIHHEADPVKASLAAVKLVHDGYAQIFMKGYIHS
                     DDFLRAVLDKEFGLRTDDLLSHIFILEATHLGRLLFITDGAMNIAPTLEQKAQIISNA
                     VAL"
     gene            complement(4571..5143)
                     /locus_tag="PLY93_03470"
     CDS             complement(4571..5143)
                     /locus_tag="PLY93_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68568.1"
                     /translation="MFKDFGDIIVPDGDTVGRTYQIKAFVRTNTMGTTEDNQTILLSA
                     SPSQDFLVTETSSNLLPSISSFQQSTATIIVVEATEIQGKSNYNFSTLTVNTVFLGTP
                     SVYATDVNGRIDKNYAASIQLELRKSINCSTVVAGELANPTDGLNLPAVAGVATFSNL
                     QFTAPGTALRIYATSSPLIPLCALPIDATL"
     gene            complement(5243..5503)
                     /locus_tag="PLY93_03475"
     CDS             complement(5243..5503)
                     /locus_tag="PLY93_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68569.1"
                     /translation="MKRINFYLILLLFSNGCITQEPIGIIFTENGVGAGIASGMVEKT
                     ITTAPATISSITNAIGSSMILLEFTVKENGSRDGYPLQINSL"
     gene            complement(5503..6207)
                     /locus_tag="PLY93_03480"
     CDS             complement(5503..6207)
                     /locus_tag="PLY93_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68570.1"
                     /translation="MFDAFFKIADQLGEKMQSALPSQRELQESGGSYYDPEKGKRTIA
                     LLFGARALRMGDATQALSSASTFSNADYPHLNAELLFQRHNVMEFLNHRSKLPVFLQF
                     SLNGGYGQQTYTRDGKNVPSKLISVEALPAVGYTFVLSKFRIEPYLGFGVGYSRFNFD
                     YSNLTRRPVDTTVAKSVSESFIEQYYTFSQVGAIVKYALRTRIAIMLAPAMTVFHFST
                     GMQVEIHGRLGVGFYF"
     gene            complement(6188..6550)
                     /locus_tag="PLY93_03485"
     CDS             complement(6188..6550)
                     /locus_tag="PLY93_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68571.1"
                     /translation="MLILPIVNDGDPANAYIGASLTEALRTRLLEIYFFIHPDNKAII
                     ATQTDNLIQDEDFFTKTVALQMGVWLRQDLVLVGKYNVRDSRLKYRFVLYEIQHEKPI
                     LSLEGETPAHRKNVRCLL"
BASE COUNT         1844 a         1609 c         1724 g         1895 t
ORIGIN      
        1 aagtaagcaa tccgcacacc cttttgaatg tcgaccttgc cttcgcttaa atgctcgcga
       61 ccagccaaca ccttgagcag cgttgacttg cccgagccgt ttgcgccggt gatgccgtaa
      121 cgtacacctt cgttaagttg gatcgagact ttctcgaata aatcgcgcgc cccgaaagac
      181 acgcctgcgt tctgtagtga aatcatagtt gcgaaatcat attctcccaa aaactagatg
      241 cggcaaaatg cacgtttaag tcataagaat cggcgatagt ctcggcgata tcggcgagag
      301 tgaggcggat gcccaagttt gcttggcggc gtggacggaa catccgcgaa tacaccaaga
      361 gcggtacgta ttcgcgcgtg tggcaagtat cttctgcggt gggatcgttg ccgtgatcgg
      421 acacgatgta aagcaagtcc tcgttttcca tcgcgcggaa aaggcgcggc aagtattggt
      481 cgaacgcttc caaggcgtcg atataaccaa tcacatcgcg gttgtgcccg tagagtgcat
      541 cgagatcggg gagggttgct aagattatat tttgtttgta tcgattgact tcgccttcgc
      601 ggataatttc gtggagcacc tcaagacatt cagcattcga cttcacgtct ttcgtttcag
      661 tgatcgccgc gccttcaatc atttcagaaa ctttgccgat ggtatgcaca gggatgcctg
      721 cgttgtgggc gtatacggcg agcgtcggtt gttgcggcgg cgattgaaac atcgctttag
      781 gcgaactatc gtcgatacgg aaaccagcgg catctccgac aaaataatgc acgtgcaatc
      841 gtcccagtcc atattttgac aacacttccc acgcgttgtg gcccaacaaa taaaactcat
      901 cgggttggaa tttatcgaga tgcacataga tatggattag cgaatcgtcg gtcaccaata
      961 aaataggatt tccagtttcc aagtggatat cgccatactt ggtgaggaag ccttgctgca
     1021 catcatttgc gttacccaaa aaaggcttac ccaccgcttt caccaattcg gccatgattt
     1081 cagagggaag cccattaggg tacgttaagt atttatcttc ttgatatgta cccaaaagtt
     1141 cgcggtgacc ggcgatggag tcgttgcctt cgttgcgctg ggttattttt ccgtaaaaac
     1201 caatcggttc ttcttcgcgt ggatggcctt tgaggtaagt tagattcgag atacccatgc
     1261 ggacaagatt atgaattttg agcggtgcgc gcatttcggc gatgtgcgca agcgtgtttg
     1321 cgccgcgctt tccgtaaaga taagcgtcgg gttgctcgcc tacgccgaag ccgtccaaaa
     1381 caagcacaat agatttaccg atgccgagcg ccacaaaatt agtcgaggtt attcattgat
     1441 gttatttcaa agcgacgatc gagcaaccct ttgagactaa ttgctttaaa gaaaatgata
     1501 acatttacta agtttatacc attggaagtg tcgtgtcaag ccctttacgg acaaacgaga
     1561 ggagtaatct gttgacagcg ggtttgtaac tcaatctatt tgtactatga aatcgtttac
     1621 tgttactggc gatacaccag agaaatttat gcgaaaagtt gagcgcgcac tttataaagg
     1681 tgagctcggt aaattttcta aaatggtttt agccatgcct gatgctaaaa ttgcctttga
     1741 aaaaatggga gtgacagagt tttcgttcaa aatttctcaa gacggtgaaa cttttacagc
     1801 acaaaaagtc gacgaaaaag ttgcggtgac ccatattttt tttagaaacg aggtcgagcg
     1861 tgaattagaa agaataattg cgcggtttgg gggcacaatt aaaaaatgac tatactcgcc
     1921 gctcaaatgg gcgaatatgg actactatga atatacaaga agatttaaaa aaatacatag
     1981 ataaagcaag ttcgcaaatc gacatcggca aagaaaaact cgaaggcttg gttgcctcgc
     2041 tggcgttaga cgaaaagaaa gcgcatttag atggcatcat tcaaagcctc aaagacaagg
     2101 gcttcaaaga aaccgtcgct tcttggattg gttcaggaga aaaccaagcg attgatcctg
     2161 aaaaaattaa agaagcgctg ggcgtgcata aaattgaaga gcttgcagcg caggctaaaa
     2221 tgaaagcgtc agaagtgcca caagcgctat ccagtttgct gccacaaatt atcgataaac
     2281 tttcccccga aggcaaagag cccgagtccg gtcttgctgc aacggcagta aagtttctaa
     2341 aaggaataca ggcaaaagtt tagtcgagcg ctaaagagcc ttttatttgg ggcgcatgtg
     2401 gcaggcgtgt agcgcaaagc ctgtttccgt aggatagcat ccacacccaa cttgtagaag
     2461 acgtgtgcct caagaattcg ttacgactgc gttagcttga gcgccgcttc tgcaatcact
     2521 ttttcttcgt cggtttccac aacaactacc ggcggcaagt tcaaatgttc aagccctgca
     2581 caaatttttt cacggataat ttttgagttc tcgccaatac ccgccgtaaa aatgatagcg
     2641 tgtggtttgc cgtgcatcag atacgcgcct aaatatttac gcacccgttc gacaaaaatt
     2701 tggagcgcaa gtaacgcaga tgcatcgcct tctgtagcgc gtttttcaat ttcacgcatg
     2761 tcgtttgtgc cgcaaagtcc tttgagtccc gattctttgt tgaggagtgt gtccatttct
     2821 tttggggtga gcttttctgc gtcttgcaaa aagagaagca atcctgcgtc gatgtcgccc
     2881 gagcgcgagc ccataacaag tccctcgagt ggcgtaaatc ccatgctggt gtcgatgctt
     2941 ttgccattac gcacacaaca aatactcgcc ccattcccta agtggagtga aattgcgtta
     3001 aactcgttgt aggggatatt catctgcttt gcgcactcgc gtgtaacgta ttcatgcgaa
     3061 ggaccatgga aaccatagcg acggatatta tatttttgtg aaagccgagg cgggattgca
     3121 tacgtcgttg caaccggagg catggtttgg tggaacgctg tgtcaaacac cgctacctgc
     3181 ggcacgttaa agcgttccat tgagtattca atccccgcta aattggcggg attgtgcaac
     3241 ggtgcgaaaa tgcaatattc gcggatggtt tggatgacgc tgtcgtcgat gatcacgggc
     3301 tcaaaaaatt ttaatccccc gtgcacaaca cggtgtccaa tcgcggtgag ttcttcggct
     3361 ttgagcacgc cgcgcctttc aaagtcggca acgataccta aaattgcttc tttatagtcg
     3421 ctaaaatttt cggcaaggga cttcccacta aattggagtt ctcccttttc ttgagtaaag
     3481 acatccgcct tgatggtcgc cgaaccacag ttgatgacta aaatcttcaa aaatgaacct
     3541 tgcctatttt aacgcgtttt tttatattca cgcccgctgc gtacaccgca ctcatgatgc
     3601 ctgcaaactt cgaggctttg gagtcggcgc gtgacggtaa aatgaccggt acctttgcgc
     3661 cgaaaataac acccgccgcc gtgccgccgg tgatgtgcgc gaacgtcttt gcgagcgcgt
     3721 tccccgaaac aatgtcgggc acaagaagga tgtccgcttg gcctgcgacc gggccaccaa
     3781 tttttttata cttggctgcc gccggattga tggcgttgtc gagcgcaaac ggaccatcga
     3841 cgataccccc cgaaaactgg ttgcgatcgc tcattttggc gagagctgca gcatccattg
     3901 tcgacacaat tttgggagta actacctcga ccgccgannn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agcgctaccg cattactgat aatttgtgct
     4021 ttttgttcga gggtgggggc gatgttcatt gcgccgtcgg tgataaaaag aagcctgccc
     4081 aagtgtgtcg cctctaaaat aaaaatatga ctgagtaggt cgtcggtacg caaaccaaat
     4141 tctttgtcga gcaccgcgcg caaaaaatca tcgctgtgga tgtagccttt cataaaaatt
     4201 tgtgcgtagc catcgtgcac cagttttacc gccgcgagcg acgcttttac agggtcggct
     4261 tcgtgatgaa tttcgatacc gcgcagatcg agtttttctt gcgctgccac tgtatgaatt
     4321 ttttcttcgt cgccgacaat caccgtttcg gcgatgtgtt tatcgcgcgc ttcggtgacg
     4381 gcgtagagag tttcggaatc gttccccgct gccaccgcga tacgcaacat ggggtactcg
     4441 aatattttct tttcgacttg ggcaaagttt ataatcacgg gttgccatct tcaaattgtg
     4501 agtgtgttgg caagaactaa gggcttttgt cggtgatcaa aataaaataa tatttcctga
     4561 cgcagttacc ttagagggtc gcatcaatag gcaaagcgca caggggaatt aaaggactgg
     4621 aagtggcgta aattcgtaaa gctgtacctg gtgcagtgaa ttgcaggttg gaaaaagttg
     4681 ctacaccggc gaccgcaggt aggtttaagc catccgtcgg atttgcaagt tcaccagcca
     4741 ccacagtcga gcaatttatt gattttcgta attccaactg aatacttgct gcgtaattct
     4801 tgtctatgcg accgttgaca tcggttgcat agaccgaagg cgttccaaga aacactgtat
     4861 tcacagtcag cgtactaaaa ttgtaattgc ttttgccttg tatctcagtc gcctccacaa
     4921 ctattatggt cgccgtactc tgctgaaatg aactaatcga cggcagcaga ttcgatgatg
     4981 tttcggtgac gagaaaatct tgggaaggtg atgccgaaag taggatagtt tggttatctt
     5041 ccgtcgttcc catagtgttg gttcgaacaa aagcttttat ttggtatgtt cggcctacgg
     5101 tgtccccgtc cgggacaatg atgtcaccaa aatctttgaa caaaattatt tgccccgatg
     5161 aaattccaaa ggtgttgttg ctccccggtc cttcgagtat aaagcggaag tctgcagcgt
     5221 ctgccgtgcc cgtgttcgtg aattacagcg agttgatttg caatggatag ccgtcgcgcg
     5281 agccgttttc tttgacggtg aactcaagca aaatcatcga cgagccaatg gcgttggtaa
     5341 tagaactgat cgttgccggt gcggtggtta ttgttttttc caccatccct gaagcgatgc
     5401 cagctcccac gccgttctcg gtgaaaataa tgccgatggg ctcttgagtg atacatccgt
     5461 tagaaaataa aagtaatatt aaataaaagt taatacgttt cattagaaat aaaaccctac
     5521 gccaaggcgc ccatggatct caacttgcat ccctgttgaa aaatgaaata ccgtcattgc
     5581 tggcgcaagc attattgcaa tgcgcgtgcg caaagcgtat ttaacaatag cgcctacctg
     5641 tgagaaagta tagtattgct cgataaaact ctccgaaaca cttttagcca cagttgtatc
     5701 gacaggtctg cgtgtgagat ttgagtagtc gaaattaaac cgtgaatagc cgaccccaaa
     5761 gcctaagtaa ggttcgatac gaaattttga aagcacaaat gtgtagccaa cggcaggcaa
     5821 cgcttcgacg ctaataagtt tgcttggcac attttttcca tcgcgtgtgt aggtctgctg
     5881 tccatacccc ccattgagag aaaattgcaa aaagacgggt agcttgctgc ggtggttcaa
     5941 gaactccatc acattatgcc gctgaaataa aagctcggcg tttaagtggg gataatcggc
     6001 attagaaaaa gtggatgcgg aagaaagcgc ttgggtggca tcccccatgc gtagcgcccg
     6061 tgcaccaaac aaaagtgcaa tcgtgcgctt gcctttttca gggtcgtagt atgagccacc
     6121 gctttcctgc aattcacgct gcgacggcaa cgccgattgc attttttcgc ccagttgatc
     6181 ggcaatctta aagaaggcat cgaacatttt tgcggtgagc gggggtctct ccttcgagac
     6241 tcaagatggg cttttcatgt tgtatttcgt acaaaacaaa ccggtatttg aggcgactat
     6301 ccctaacgtt gtacttgccg acgaggacta aatcctgtcg cagccaaaca cccatttgta
     6361 gagcgacagt tttagtgaaa aaatcttcgt cttggattag gttgtcggtc tgcgtcgcta
     6421 taattgcttt attgtctggg tggataaaaa aatatatttc taaaaggcga gtacgcaacg
     6481 cttcggtaag cgatgcgcca atatacgcat ttgccggatc gccgtcgttg acaatgggta
     6541 gaattaacat ttttttgacg gtaggtgcag caaaaacatg cgaatatagc actgtggcaa
     6601 gtagaaaagt aacgattatg gattttttta ttaatactcg tcgtattggg attgagagaa
     6661 tgttcatttt ctactttggt ttttataacg ttcccgaacg tacaggctct aaaacgtatt
     6721 gtaacgaggc agatctcgtg gaaattaaaa ttttcaggaa ctatttatcc cattttgagg
     6781 tctacaagat taacacggca aaggttacct cctcccaagt gcgattttag cctgccttca
     6841 cctccctgtt ggcagtgcac ttcgtactcc cgtgtgcaac atctgctata gcaaatagta
     6901 gatcggcatt gaatgccgca aaactccgaa gtttggcggc ggtaaggagt ctcctgcctt
     6961 gccgccgctt ttttatatgc gtttacggcg tgtctcattt attttttagt ctacgatctg
     7021 aaattctcga aaaacatcgg tatcttgggc ggcggccaac tggcgcaaat gacgatcgct
     7081 gcggcgatgc ggctgggcgt gcgttgccat gtactcgata aaga
//
LOCUS       DAOMPD010000065         7097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4525_length_7097_cov_3.007526, whole genome shotgun sequence.
ACCESSION   DAOMPD010000065 DAOMPD010000000
VERSION     DAOMPD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4525_length_7097_cov_3.007526"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLY93_03490"
     CDS             complement(<1..568)
                     /locus_tag="PLY93_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68572.1"
                     /translation="MKKNRFVAILLASSISTPLLFSADALEKFRQGVDKFKGGDYAAS
                     QKELSEFIKEHPYEVEVKKSWYYLARIAAAQKKYKDAIERINITLSRYPRHEDRVELN
                     VLLGECLYEVNAHAHAEKVLQSVRTSAKDEALLYRLEKKLGFIAYAARRDAKTVEHLK
                     AALRLAEKNAPHDSELFNVYATLGKVTAK"
     gene            complement(565..1053)
                     /locus_tag="PLY93_03495"
     CDS             complement(565..1053)
                     /locus_tag="PLY93_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRP68573.1"
                     /translation="MFIERIGQILARSREHWLFIPFFVLLILSFVYVFFIGDKGFLAY
                     RVKLQEREDVKAQIEDFTKQKKDLREKLVMLRDKDQAVNKFTKDFYLFQEKVRILKFR
                     EEAEDKPQETSEAADLATMQKFYIIFSTLLIGLATLFFYRRNTARMNMEKVRPFDLGD
                     HP"
     gene            complement(1071..1865)
                     /locus_tag="PLY93_03500"
     CDS             complement(1071..1865)
                     /locus_tag="PLY93_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68574.1"
                     /translation="MLRTLIFAALVSTPLLFAQSNEKKSATGEATPESQKGSGLIRDE
                     RIAVENLSFWKKFAADGKGDYLEFTFDIVNKTEDEIPLKMFIIAFNERDLVDSEYRRY
                     VEYPAWRKWDEDKAAHKIVLYNSIPETKHEDVAAFARKKEEAIAKNGGFKPREEEPQP
                     TGSKKKQPTLKQFLDYVQYIHENPTTGTDVLLQGFEDAKFTRKVEPTLRIEEKALKTN
                     VWGKLLARYRVDRKFFNHFGIILYDTEAKKIVHRQFYSINGKFKIY"
     gene            complement(1907..2545)
                     /locus_tag="PLY93_03505"
     CDS             complement(1907..2545)
                     /locus_tag="PLY93_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRP68575.1"
                     /translation="MSGGLFNKFGKQYNTGEIIFCEFEPGNSFYLIQTGRVKISKVVK
                     DKEKTMDILSVGDIFGEMAILEMQPRSATATAIEPVTVLHFDRENFVSMMMSQPQLAF
                     KLLVVFSKRIYDAKRRLMILLLDDIQSKVADVFLMLAEKEPSFANMREVAFPVTVDDV
                     ANWAGASHDQVSEQLNHWAKVGKIELYSDRIFVRNMVDFKRIVSQKRKTEKR"
     gene            complement(2542..3726)
                     /locus_tag="PLY93_03510"
     CDS             complement(2542..3726)
                     /locus_tag="PLY93_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP68576.1"
                     /translation="MPITRNYSAGSIVYFAGDVGEDVYVMQKGRISLISTSLDQKEDI
                     KEDVHKGEFFGVKSALGRYPREETAQVLADSQVLVFKLAEFEQFGQKNTRIVLQMLKV
                     FSSQLRRVHKAVREFLGEHGAVESSMELVGVGEHYYKIGKTDYALYVFDAYMKHYPSG
                     PLIERVRKLAESVKQGAPFPLDLPDLAEEMQRNEVASRTTAMMGRIEQDPVDSYDTEV
                     EPPTSPIPDADDLPSLEMPSNDMDMGELPPPGDFDLSMPNDFDLPAEKSAAEFFNEGL
                     SAYAGQRFDEALHAYQEALSAVGTSDAALHEKILFELGRTFAKKGNGKGAVEKFSELM
                     RTYPSGTFAKKTLIQLAEIYERARDTQRAIEFYGKAAKIPPQDKEANIAQQKANQLKG
                     TR"
     gene            complement(3726..>4047)
                     /locus_tag="PLY93_03515"
     CDS             complement(3726..>4047)
                     /locus_tag="PLY93_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRP68577.1"
                     /translation="CLPDAGEISEGKTADLLLIDGNAANPWATLTHADYADVMLVIAD
                     GMPRYGDVAFQPVFDAMKIPYQTILVKGARKIIAGDILGLQKRVRHAVKFKKELDFLP
                     IEAA"
     assembly_gap    4048..4057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4058..4961)
                     /locus_tag="PLY93_03520"
     CDS             complement(<4058..4961)
                     /locus_tag="PLY93_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP68578.1"
                     /translation="MAWELVNGTIVTAREVIERGSITVDKTRIAQVAKSSRKNAEAMQ
                     IDVNGMLVFPGLINCHDHLLGTYVPRVGDRRPYLNWLEWDNDLKSAAAYAERQQIDSS
                     DLYLMGGFRHLLCGITRVQDHIPHFVRELFAKNMPIRIISDFAMAHSVTSFALPWGDG
                     IEIEHAKAVEKNIPFITHCSEGYDEETLKSVETLAKKGALSKQTVLIHGIAFSDKDIE
                     LLAENQCNVVWCPVSNLYMFEQTARVKELMDAGVNVCLGTDSPMSGSFSIFEELRTAR
                     EFYKFAYGKNIDDRILVNMLTVNAA"
     gene            complement(4961..5326)
                     /locus_tag="PLY93_03525"
     CDS             complement(4961..5326)
                     /locus_tag="PLY93_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRP68579.1"
                     /translation="MLAFENKPSFVDFRKMGSVVKKYSTKRGNPQYGVTLKLRSNSIV
                     ALADGRIAGLGHLRGYGLYVIVDHGSGWHSLYSHLSRVDRVTGSSVKRGASIGVARNK
                     KLFLVVSYRGNPINPSEVF"
     gene            5496..5903
                     /locus_tag="PLY93_03530"
     CDS             5496..5903
                     /locus_tag="PLY93_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP68580.1"
                     /translation="MAKYDQTNSVIGEGSIFEGRFYISGSLRIDGKFEGDIRTDEALV
                     VGETGKVKTNISAREVVLAGTLIGDIDAKDEVRLTETGRMVGDISSPILNLSRGVVLK
                     GNVSIHGGHKKDPRKIVEESYGLVRELDKKGIQ"
     gene            5929..>7097
                     /locus_tag="PLY93_03535"
     CDS             5929..>7097
                     /locus_tag="PLY93_03535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP68581.1"
                     /translation="MAAKKALKVSSGTGRKSKQGNTMRREPSKNKTKSDFFVLEPNKS
                     TLNKKSETPSVSKPTPISWSDAPTKKPFLASLRINLGFIEFAHQGHGKFLYFYRRHNT
                     LKNGVLQITRRTLLVSLCAFFGVVGVTTTLLWKKRQHAMEIAFDDDKRGVGGVGRLES
                     GKVIATSTDFVEELAQFEDDEKMKDQLMGALEEKTESDKAEKRSHKRVQKLKYKVKRG
                     DTLQSVAKKYNVRPESISGSSRLNEFQEISPGQTLSIPTRDGFYYVVKKNERLANILQ
                     AHKVTLDKFIAENEHVNIDLLDSGEEIFLPGAKPIRRAEGWLVPVPSRIITSGYGRRS
                     FPRLAFHRGLDLKAFYIPFRAAKSGVVTYSGWLGGYGNAIIVKHDDTYKTLYAHLS"
BASE COUNT         1861 a         1719 c         1595 g         1912 t
ORIGIN      
        1 cttttgccgt gacttttccc aacgttgcgt agacgttaaa aagctcgctg tcgtgcggtg
       61 cgtttttttc cgcaaggcgc agcgcggcct ttaaatgttc gacggtcttt gcatcgcgtc
      121 gcgccgcgta cgcaatgaac cccaattttt tttcaagccg atataacagc gcttcgtctt
      181 tagcggaagt acgcacgctt tgcaaaactt tttcggcgtg ggcgtgtgca ttaacctcgt
      241 agaggcactc gcctaaaagc acattcaact caacgcgatc ttcgtgccga ggatagcggc
      301 tgagcgtgat gttgatacgc tcaatagcgt ctttatattt tttttgggca gcagcgatgc
      361 gagctaaata ataccacgat tttttgacct cgacttcata tgggtgttct ttgataaatt
      421 cgctcaactc cttttgagac gcggcataat ctccgccctt gaacttgtcc accccctgcc
      481 taaatttttc aagagcgtcg gcagaaaaaa gtaacggcgt ggaaatcgac gacgccaaaa
      541 ggattgcaac aaatcgattt tttttcacgg atgatctccc aaatcgaaag gccgcacttt
      601 ttccatgttc atgcgagctg tgttgcgtcg gtaaaaaaat aacgttgcta gtccaatcaa
      661 taacgtcgaa aaaataatat aaaatttttg catcgtcgca aggtctgctg cttcgcttgt
      721 ttcttgcggt ttatcttccg cctcttcacg aaatttcaga atgcgcactt tttcttggaa
      781 caaataaaaa tcttttgtga atttattaac cgcctggtct ttatcgcgca gcattactaa
      841 tttttcacgc aagtcctttt tttgttttgt aaaatcttca atttgcgcct ttacatcttc
      901 ccgttcttgt agttttacac ggtatgcaag aaagccttta tctccgataa aaaaaacgta
      961 cacgaaggaa agtataagca acacaaagaa aggaataaac agccaatgtt cgcgactgcg
     1021 cgcgagaatt tgtccaattc tctcaataaa catgaatcat ttaattcgtt ctaatatatt
     1081 ttaaatttac cgttgataga ataaaactgg cggtgcacaa ttttcttcgc ttcggtgtcg
     1141 tagagaataa taccaaagtg gttgaagaac ttgcgatcaa cgcgatatct tgccaaaagt
     1201 ttaccccaca cgttggtttt caacgccttt tcttcgatgc gcagcgtggg ctcgactttg
     1261 cgcgtaaact ttgcatcttc aaacccttgc aaaagcacat cggtgcccgt cgttgggttt
     1321 tcgtggatgt attgcacgta atctaaaaat tgctttagcg tgggttgttt cttctttgaa
     1381 ccagtgggtt gcggttcttc ctcacgcggt ttgaatccac cattttttgc aatcgcctct
     1441 tcttttttgc gagcaaatgc cgctacgtct tcgtgcttcg tttcagggat gctgttataa
     1501 agaacaattt tatgcgcggc tttatcttca tcccacttac gccaagcggg gtattcaacg
     1561 taacggcggt attcagagtc gaccaaatcg cgttcgttga acgcaatgat aaacatcttt
     1621 aggggaattt catcttctgt tttgttgaca atatcaaaag tgaattcgag ataatcgcct
     1681 ttaccgtcgg cggcaaattt tttccaaaaa gaaagatttt caacagcgat gcgttcgtcg
     1741 cgtatcaagc cgctaccttt ttgcgattcg ggcgttgctt cgcccgttgc gcttttcttt
     1801 tcattcgatt gcgcaaacaa aagcggtgtg cttacgagtg cggcaaaaat aagtgtgcgt
     1861 aacatagttt cccctatttg cgtatcggca tattacaagc cataatttat cttttttccg
     1921 tttttcgttt ctgcgaaacg atgcgcttaa agtcgaccat gtttcgtaca aaaatacggt
     1981 cgctataaag ttcaatctta ccaactttcg cccagtggtt aagttgttcg gacacttggt
     2041 cgtggctcgc gcccgcccag tttgccacat cgtcgacggt cactgggaac gcaacttcgc
     2101 gcatgtttgc aaacgacggt tctttttcag cgagcattaa aaacacatcg gcgactttcg
     2161 actgaatgtc gtcgaggagt aaaatcatga gacggcgctt cgcatcgtaa atgcgttttg
     2221 aaaaaacaac caaaagtttg aacgccaact gcggttgact catcatcatc gacacaaaat
     2281 tctctcggtc gaaatgaagc actgtgaccg gttcgatggc agtcgcagtg gccgaacgcg
     2341 gttgcatttc gaggattgcc atttcaccaa agatatcgcc gacactgaga atgtccatcg
     2401 tcttttcttt atctttgacg acttttgaga tcttcactcg accggtttgg atgagataaa
     2461 agctattccc gggttcaaac tcgcaaaaaa taatttcacc ggtgttgtac tgcttaccaa
     2521 acttattgaa aagtccgccg ctcatcttgt ccccttgagt tgattcgcct tctgctgcgc
     2581 aatgttggct tctttgtctt gtggcggtat cttggcggct ttcccgtaaa actcaatcgc
     2641 tcgctgtgtg tcgcgcgcac gttcataaat ttcagccaat tgaatcagtg tcttttttgc
     2701 aaaagtaccg ctggggtatg tgcgcatcaa ctctgaaaat ttttcaaccg cgcctttgcc
     2761 gttccctttt ttagcgaaag tgcgtcccaa ttcaaaaaga attttttcgt gcaacgccgc
     2821 gtctgaagta cccacggcgc tgagcgcctc ttggtacgca tggagcgctt cgtcgaaacg
     2881 ttgtcccgca tacgcgctca acccttcgtt aaagaattcg gcggcagatt tttctgcggg
     2941 taaatcgaaa tcgttgggca tcgataaatc gaaatcacca ggggggggca actcacccat
     3001 gtccatatcg ttggaaggca tttctaaaga aggtaaatca tcggcgtcgg gtatcggcga
     3061 cgttggaggc tcaacttcag tgtcgtaact gtcgacgggg tcttgctcga tgcgccccat
     3121 cattgccgtt gtgcgcgacg ccacttcgtt acgctgcatt tcctcggcga ggtcgggcaa
     3181 gtcgaggggg aatggcgcgc cttgctttac cgattccgcc agtttgcgca cacgttcaat
     3241 aagcggccca gagggataat gtttcatgta cgcatcgaag acataaagcg cgtaatcggt
     3301 tttaccaatt ttgtagtaat gctcgccgac accgacgagt tccatcgacg attccactgc
     3361 accgtgttcg cccaaaaact cgcgcactgc tttgtgcacg cgccgcagtt gcgatgaaaa
     3421 tactttgagc atttgtaaaa caatacgcgt atttttttgt ccaaactgtt caaactcggc
     3481 gagtttaaag acgagcactt gcgaatcggc caaaacttgc gccgtttctt cgcgcgggta
     3541 tctacccagt gcagatttga cgccgaaaaa ctcgcctttg tgcacatctt ctttaatgtc
     3601 ttctttttga tcgagagagg tggaaataag actgatgcgt cctttttgca tcacgtacac
     3661 gtcttcgccc acgtcgccgg caaaataaac aatcgaaccg gcagagtaat ttcgggttat
     3721 cggcattatg cggcctcgat aggcaagaag tcgagttcct ttttaaactt aacagcgtgg
     3781 cgtacacgct tttgcaatcc caagatgtcg ccggcaataa tttttcgggc ccctttcacg
     3841 aggatggttt ggtagggaat tttcatggcg tcaaacacgg gctgaaaagc aacatcaccg
     3901 tagcgcggca tgccatcggc aatgacaagc atgacatcgg cgtaatcggc atgcgtgagc
     3961 gtagcccatg gattcgccgc attgccgtcg ataagaagta aatccgccgt tttaccttcg
     4021 ctgatttcac ctgcatcggg aagacacnnn nnnnnnnttg cggcattgac tgtcagcata
     4081 tttactaaaa tacgatcgtc aatattttta ccataagcaa atttgtaaaa ttcgcgcgcg
     4141 gtacgcaact cttcaaaaat actaaaactg ccagacatag gtgaatcggt gccaaggcaa
     4201 acattgaccc ccgcgtccat gagttcttta acgcgtgcag tttgctcgaa catgtagaga
     4261 ttagagacgg ggcaccacac cacgttacat tgattttcgg cgagcaattc aatgtcttta
     4321 tctgaaaatg caatgccatg gatgagcaca gtttgtttgc tgagcgcccc tttcttagca
     4381 agcgtttcaa cgctcttcaa tgtttcctcg tcgtaacctt cggagcaatg cgtaatgaag
     4441 ggtatatttt tctctactgc tttcgcgtgt tcgatttcaa tgccatcccc ccaaggaagc
     4501 gcgaaagaag tcaccgaatg cgccatcgca aaatcggaaa tgatacgtat cggcatattc
     4561 ttggcaaaaa gctcacgcac aaagtggggg atatggtctt gcacacgggt aatgccgcag
     4621 agcaagtgac gaaagccacc catcaaatat aaatcgctcg aatcgatttg ttggcgctcg
     4681 gcgtatgcag cggcgctttt caagtcgtta tcccattcga gccagttgag gtagggtcgg
     4741 cgatcgccca cacgcggtac atacgtgccg agcaagtggt cgtggcagtt gatgagaccg
     4801 ggaaagacca acatcccgtt cacgtcgatc tgcattgcct ctgcattctt gcgcgacgat
     4861 ttcgctacct gcgcgatacg tgttttatcc actgtaattg aaccgcgttc gattacctcg
     4921 cgcgcagtca caattgttcc gtttacaagt tcccaagcca ttaaaatact tccgaggggt
     4981 ttattggatt gcctctgtag gatacaacta aaaaaagttt tttgttacgc gccaccccta
     5041 tcgacgcgcc ccgtttcaca gagctacccg ttaccctatc gacccgcgaa aggtgagagt
     5101 aaagcgagtg ccagcccgag ccatggtcga caatcacata aagtccatag ccccttaaat
     5161 gtcctaagcc cgcaatgcga ccatcggcta acgcgacaat cgaattgctg cgcaacttta
     5221 gcgttacgcc atattgtgga ttcccccgct ttgttgaata ctttttaacg acagaaccca
     5281 tttttctaaa atcgacaaac gagggtttgt tttcaaaggc cagcaaagga tgagcggtgc
     5341 atcctcctat taaaaagaat agcaaaccaa ctgtcgatac gactcgtcgt gtgagcatat
     5401 tatagtttcg gcatcggaag ccttgaaaat gaattttagt tctcacacag cctcaaagct
     5461 aataattgac gcagaattac tatataaggt acagtatggc taaatacgat caaacaaact
     5521 cagtcattgg tgaaggttca atttttgagg ggcgattcta tatatcgggc agtcttcgca
     5581 tcgatggcaa atttgaaggc gatattcgca ccgacgaggc gttggttgtc ggcgagacag
     5641 ggaaagtaaa aacaaatatt tcagcgcgcg aggttgtttt agcgggcact ctcatcggcg
     5701 acatcgacgc aaaagatgaa gtacgcttaa ccgaaactgg ccgtatggtg ggcgatattt
     5761 catcgcctat tcttaattta tcgcgcgggg tggttcttaa aggtaacgtt agcatccacg
     5821 gtgggcataa aaaagaccca cgcaaaattg tcgaagaaag ctatggctta gtacgcgaac
     5881 tcgacaagaa aggaattcag taaaaagatt taataacggt agaatacctt ggcggcaaaa
     5941 aaagcactca aagtttcaag cggtactggt cgaaaatcaa agcaggggaa taccatgcgc
     6001 cgcgaaccat cgaaaaataa aacaaaatct gatttttttg ttcttgaacc aaacaaatct
     6061 accttgaaca aaaaaagtga aacaccgtcg gtaagcaaac caacaccgat aagctggagc
     6121 gacgctccca cgaagaaacc tttccttgca agtctgcgca tcaatttggg tttcatcgag
     6181 tttgcgcacc aaggccacgg taagttttta tatttctacc gccgacacaa tactttaaaa
     6241 aacggtgtac ttcaaattac ccgccgcacg ttacttgtct cgctttgcgc ttttttcggt
     6301 gttgtaggag tcaccaccac tctactctgg aaaaagcgcc agcacgcaat ggaaatcgca
     6361 tttgacgacg ataaacgtgg cgttggtggt gtgggtcgtc tggagtcggg caaagtcatt
     6421 gcaacctcaa ccgatttcgt cgaagagttg gcgcaatttg aagacgacga aaaaatgaaa
     6481 gaccagctca tgggcgcgct cgaagaaaaa accgaaagcg ataaagccga gaaacgcagc
     6541 cacaagcggg tgcagaaatt aaaatataaa gtcaagcgcg gcgacacact acaatcggtt
     6601 gcaaaaaaat acaacgtgcg tcccgagtcg attagcggct caagtcgatt gaatgaattt
     6661 caagaaattt cacccggaca aacactatcg atacccaccc gcgacggttt ttattacgtg
     6721 gtgaaaaaaa acgaacgctt ggccaatatt ttgcaagcgc ataaagtaac cctcgataaa
     6781 tttatcgcag agaacgaaca cgtcaacatc gatcttttag actcgggcga agaaattttt
     6841 ctccccggcg caaaaccaat acgacgcgca gaaggctggt tggtgcccgt gcccagtcgt
     6901 atcatcacct ccggttatgg ccgacgcagc ttcccccgtc tcgccttcca ccgtgggctt
     6961 gaccttaaag cattctacat tcctttccgt gctgcaaaat cgggcgtagt tacctactcg
     7021 ggttggctcg gtggctatgg aaacgccatc atcgtcaaac acgacgacac gtataaaact
     7081 ctctatgcac acctttc
//
LOCUS       DAOMPD010000066         7048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4586_length_7048_cov_2.477334, whole genome shotgun sequence.
ACCESSION   DAOMPD010000066 DAOMPD010000000
VERSION     DAOMPD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_7048_cov_2.477334"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            620..1006
                     /locus_tag="PLY93_03540"
     CDS             620..1006
                     /locus_tag="PLY93_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68582.1"
                     /translation="MKYLTSYSYPIAAILFGIITLVEGGNTIKSVPTATMLSQSIVPV
                     VLYFNFTSGFLYIAIGIWALKSRFASRYGATTLALLIFVVGVYLTVHILSGATYMPRT
                     VGALALRFIFWSGFIFWAQKNKTGQR"
     gene            complement(1057..4305)
                     /locus_tag="PLY93_03545"
     CDS             complement(1057..4305)
                     /locus_tag="PLY93_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP68583.1"
                     /translation="MLDIAGVSVPEAEVSPTEKNLVFHQELILTETTQANLRKILYPL
                     LEGQNLLLVGDAGVGKNALIYYINRIRGLPTVRFSFNQDTLPEDLSGSFRVLPSGFQW
                     NNGPLAEALEKGYTFVADEMNLASPEILKRFISVFDRRRLALLEKDGSDIHASDRFSF
                     VATQNPARGFEGRKNLPESIARHFTTIYLDHYPEHEEKEILRGLYPAEYGAIADIVVK
                     VQRVLETAIWKNELAKEDLEHYHFNLRTAQRFMNRALVASVADANTPVFRDLLFTFYV
                     EVFRLEADRENALKIIAQVLTYDWAQLKGDYEISRVSTAHGAYNNQVDLRGEDKKLLS
                     QLYFPLTSTRQTILEKMHASLTANDNLLLEGDDAARILELCHRYCDAYQKNQRVTEVF
                     LSRGMHTSDIIGALRPHLHGGIGSGAGMDAIGAPAMLHGGIGSGAGMDAIGAPPERQR
                     VKWIDGPLTAALRRGDLIILENIDAAGSELVEKLNMLLDHAGRLALPPEAEGESILKK
                     EGNCRIIAIKRTRRSRNQQTISRALRNRFFNMYVPVVEAQSEVQEIVALALDNEFGED
                     ASPLRKSFVLKLALFHGKADDAAREKKVGASLAESIRYREENLLRLLGHVGHAIVPSQ
                     RERGLGGEGAEEILKRGVDIYYAGILSDAEERLFIRRLFERIFADMPWEDMFTTLSEG
                     KKKILSKGKKRNKEINPPDWDKAKHFREANTGKAQKRLSGEDLKKGIRIDTPETGGNT
                     KEGPDAWYGSDTQGNGGQGEPAGGGGAWGYRNEALFEAFLKKYKPKWEYHMGYSLADF
                     YDVFGKMLSELQMDLENALDAEPEIERRLLSEGHRVDARRYITYQAYAGNDRIFDHTR
                     IRHVENKLKGVEFIFMLAKGRRMFNFKAALSAVVALQSCAEILFDRKIPIKVFGYSDF
                     DNMKRAIDINRYLATEGESPSHEEKVALFDSMTDHWNGDTVDESQVLRHAAQEFSPEA
                     TTKFVVMVSDFRGHRARAELQKEILSHQSQDLKNLGEEYERQNIHVLAVQTGTRSIAE
                     HLFKESLWIHEETFASAPILLAEGNDFEIPPRGGVKPSHLAKGHLFASGRIR"
     gene            4399..5793
                     /gene="tig"
                     /locus_tag="PLY93_03550"
     CDS             4399..5793
                     /gene="tig"
                     /locus_tag="PLY93_03550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRP68584.1"
                     /translation="MSSISVSRESDTRIKASITVNTEEIKPAEEAALKKIAAKAEIKG
                     FRRGKAPAHLVKQKYASDVATEKLFALVDNCYEDIQKKVDERLYRLVSISDLKENNDS
                     MSFAVSLDLYPYVKIGKLKTVQLTRHTAAIDDSDVNEEIKKRLMRFATYEDTENATAA
                     ANDKVTVDFEIWVDDAPSGEVNKDFQFDLGSGSLSRELEQQIIDKKGKVGEEFKLKKE
                     IPQQQEEKNRSYEIIATLKKISKPKLPELTDALVAENFKGSQTVAEFKNKVKEDLERQ
                     FDLAQIRVETDKALNTLQENSEFFISESFLETQLDEFAAERKIQKDELRPEQASGLKK
                     ALGEREHSALLMRQLLRDAEKSHQKKTKSEETYMQAFRKYVHQEIIRFENDEQKASEI
                     LVDVDRALTALEEGRSEKNPWESIISSYLIVFNRQWIFDFFEEEGLVKKGKKLTYKEL
                     AALLGSTSSGPDPL"
     gene            5790..6653
                     /locus_tag="PLY93_03555"
     CDS             5790..6653
                     /locus_tag="PLY93_03555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP68585.1"
                     /translation="MKSIKLSTILVFFLSFFIAPFLHADIGTRSSWQLMTERAEELMQ
                     EGEVEIDNRLIQTYQGYAGVWTRASGSSTVKRVCKEAMCDDELFRRVNELTESQKSLK
                     RDKLYFVTFTPEYVEKLRTEGVYRQRVTLKKGQYIWPVMGIRITSRVGGRWGRIHGGI
                     DVAAARGSVVVAATDGTVMIAGDQGAYGHCVFVENTDGTYAWYAHLTDSYVKPGDKIT
                     RGQFIAASGNTGRSTGPHLHFEMRTQQGIILDPEHFFVLPFDEHLKQAQDFESDTASH
                     GALTRFKQPAQ"
     gene            6653..>7048
                     /locus_tag="PLY93_03560"
     CDS             6653..>7048
                     /locus_tag="PLY93_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRP68586.1"
                     /translation="MLDVNYLTHRPRRNRVNSAARALVEENQIRVDKLIEPLFLLDGQ
                     NKRDSISSMPGIFRLSEDNALRHVEASLRLGIRSFILFPVIEDNLKDNVGSYSYAKNN
                     FYLRIIAALKKNFHEALLISDAALDPCSTD"
BASE COUNT         1895 a         1730 c         1633 g         1790 t
ORIGIN      
        1 agggcttggt gtgcgagttg gcgcacaggc tcctaagagg ctgtgcgcca actcccgcag
       61 cgggttggcg tacagcctct taactgcgtg taacgctgac acagcacttt tacaagttca
      121 agtacgtaag ctataccaat ttttcagatt atgtcacatt aggggataac tatgccagtt
      181 tttcggatta tgtcacatta gggaataact atgccagtct ttcgaattat gtcacattag
      241 gggataacta tgcacagcaa atgctcaact gaacaaaatc atggtaaact ttcgaggaaa
      301 aactctcttc gatacgtaca ggccatcgtt cttccccgtt tcaaccgagc tgcagcattt
      361 gtttgccaaa ttactttatc aaaaaataat ttttttgatt tacaaaagga atatccattg
      421 cattattcaa tttatgaaaa aagttgcaca ggactataac caacaagaaa cgagcatact
      481 tggaagcagg tcatgaagac aggctttcaa aaaaaatgac gatatgatta tggaaacgta
      541 cataaagaac cgtggcagtg ttatcggcat ggatgcaaaa gcacatactc gctttagcgc
      601 cgattaagta ttaaaaatta tgaaatacct tacatcatat tcatatccta tcgcagccat
      661 tttatttggt ataatcacat tggttgaagg aggcaacaca ataaagtcgg taccgactgc
      721 aaccatgctg tcacagtcta tcgttcccgt ggtgctttat tttaatttca caagcggctt
      781 tttgtatatc gctattggca tctgggcgct aaaaagtcga tttgcaagcc gttacggcgc
      841 gacgactctt gcattactca tttttgtagt gggcgtatat ctcactgtac atattttatc
      901 cggcgctacg tatatgccac gtactgtcgg tgcattagcg ctgcggttta tcttttggag
      961 tggttttatc ttttgggcac aaaaaaataa aactggacag cgctaaataa cgcttcaaaa
     1021 tatgcaaaaa atttgccgca gcgcattcgt cgtaggtcag cgtatgcgac cgctcgcaaa
     1081 gagatgtccc tttgcgagat gagaaggctt tacaccacca cgcggtggta tttcaaaatc
     1141 attcccttct gccaataaaa tgggcgcaga cgcgaatgtc tcctcatgaa tccagagaga
     1201 ttctttgaaa aggtgctctg caatcgaacg tgtgccagtt tgtactgcaa ggacgtggat
     1261 gttctggcgt tcgtattcct cacccaaatt ttttagatct tggctctgat ggctcaatat
     1321 ttccttttgt aactcggcgc gcgcacgatg gccacggaag tcagaaacca tgaccacaaa
     1381 ttttgtcgtc gcctcggggg aaaactcctg cgcggcatgg cgcagcactt gagattcatc
     1441 caccgtatcg ccattccagt ggtcggtcat gctgtcgaaa agcgccactt tttcttcgtg
     1501 cgatggactc tcgccctcgg ttgccaaata gcggttgatg tctatcgcgc gtttcatatt
     1561 gtcaaagtct gaataaccaa agactttgat gggaatttta cgatcgaaaa gaatttcggc
     1621 gcacgactgc aacgcgacca ccgccgagag cgccgccttg aagttgaaca tccgtcggcc
     1681 tttggcgagc ataaaaataa actcaacccc tttgagctta ttttccacat ggcggatgcg
     1741 cgtgtgatcg aaaatgcggt cattgcccgc atacgcttgg tacgtgatgt aacggcgagc
     1801 atcgacacgg tggccttcgg acaaaagccg gcgttcaatt tcgggttcgg catcgagagc
     1861 attttctaaa tccatctgaa gttcgctgag cattttacca aacacgtcat aaaaatcggc
     1921 gagtgaatag cccatgtggt attcccactt gggtttatat ttttttaaga acgcttcaaa
     1981 aagcgcttca ttacgataac cccacgcacc accaccaccc gcaggttcgc cttgcccccc
     2041 gttgccttgc gtgtccgagc cataccacgc gtcggggcct tctttggtgt taccgccggt
     2101 ttcgggggtg tcgatgcgga tccctttctt gagatcttcg cccgaaagac gcttttgagc
     2161 tttgccggtg ttcgcctcgc ggaaatgttt cgccttatcc caatcggggg gatttatttc
     2221 tttgttacgc tttttgccct tgctgaggat ttttttttta ccttcgctca acgtagtgaa
     2281 catgtcttcc cacggcatgt cggcaaagat gcgctcaaaa agccgtcgga tgaaaagcct
     2341 ctcttcagcg tcggataaaa taccggcata gtaaatatcc acaccacgct tgagaatttc
     2401 ttctgctccc tcgcccccta accctctttc tcgttggctg ggaacaatgg catgcccaac
     2461 atgcccaagc aagcgcagca gattctcttc acgataacgg atcgactcgg ccaggcttgc
     2521 tcccactttt ttctcgcgtg cagcgtcgtc ggctttccca tggaaaagtg cgagcttcaa
     2581 gacaaaactt ttgcgcaacg gggatgcgtc ttcgccaaat tcattgtcga gcgccaacgc
     2641 aacaatttct tgtacttcgc tttgcgcttc cacaactggt acgtacatgt taaaaaaacg
     2701 attgcgcagc gcgcgcgaga tggtttgctg gttgcggctt ctgcgcgtgc gtttaatggc
     2761 gataatacgg caattacctt ctttctttaa tatggactcg ccttcggctt caggggggag
     2821 agccaagcgc cccgcgtggt cgagcagcat gttgagtttc tccacaagct cgctaccggc
     2881 ggcgtcgata ttttctaaaa tgattaaatc accgcgccgc aacgctgcag tgagtggacc
     2941 gtcaatccat ttgactcttt gacgctcggg cggtgcacca atggcatcca tgccagcacc
     3001 gctcccgata cctccgtgta gcatagccgg tgcaccaatg gcatccatgc cagcaccgct
     3061 cccgatacct ccgtgtaagt ggggacgtaa cgcaccaata atatcgctcg tgtgcatgcc
     3121 gcgtgagaga aaaacttctg tcacccgttg gttcttttgg tatgcgtcgc agtacctgtg
     3181 gcacaactct aaaatccgag cagcatcgtc gccttccaaa agcaagttat cgttcgcggt
     3241 gagcgacgca tgcatctttt caaggattgt ttgccgcgtc gaggtgagtg ggaaatacag
     3301 ttgcgacagt aattttttgt cctctccacg caagtcaact tgattattat aagcgccgtg
     3361 tgccgttgag acgcgagata tttcataatc ccctttcagt tgcgcccaat cgtatgttaa
     3421 aacttgtgca attattttca gtgcattttc acggtccgct tctaaacgaa acacctctac
     3481 ataaaacgtg aaaagtaaat cgcggaacac gggcgtgttg gcatctgcca ccgatgccac
     3541 caatgcacgg ttcataaaac gctgcgcggt gcgtaagtta aaatggtaat gctctaaatc
     3601 ttctttagca agttcgttct tccaaattgc tgtctcaagc acacgctgca cctttacaac
     3661 aatgtcggca atagcgccgt actcggcagg ataaagaccg cgcagaattt ctttttcctc
     3721 atgctcggga taatggtcga gataaatcgt ggtgaaatgc cgagcgatgc tttcgggtaa
     3781 atttttgcgt ccctcaaaac cacgcgcagg attttgcgtc gcgacaaatg agaaacgatc
     3841 gctcgcatga atgtcgctgc cgtctttttc caagagcgcc aaacggcggc ggtcgaacac
     3901 gctgataaac cgcttcaaaa tttcaggcga cgccaggttc atttcgtcgg cgacaaaggt
     3961 atatcccttt tcaagagctt cggccaaggg accgttattc cattgaaagc cagacggcaa
     4021 cacacgaaat gagcccgata aatcctcggg tagcgtgtct tggttaaaac taaaacgcac
     4081 tgtcggcaaa ccacggatgc ggttgatata ataaatgagc gcgttcttgc ccacgccggc
     4141 atcgccgacc aacagcaaat tttgtccctc caaaaggggg tacaaaattt tgcgcagatt
     4201 cgcttgcgtt gtttcggtga ggattaattc ttggtggaag acgagatttt tttcagtggg
     4261 ggaaacctct gcttcgggga cagaaactcc agcaatatca agcatggttc tcaattcata
     4321 tctgcgttag cgtgtaaacg aggatggact tatgctgcaa cgacttgacg cgctgtctaa
     4381 aatgcttgtt ttgccagaat gtcatccatt tctgtaagcc gtgagagcga tacccgcatc
     4441 aaggcgagca tcaccgtcaa caccgaagaa attaaacccg ccgaagaagc agcgctgaaa
     4501 aaaattgccg caaaagcaga gattaaaggc tttcgccgcg gtaaggcccc cgcacactta
     4561 gtgaagcaaa agtatgcatc agatgtagcg actgaaaaac ttttcgctct tgtagacaac
     4621 tgctacgagg atatccaaaa aaaggtggat gaacgtcttt accgcttagt gagcatttct
     4681 gacctcaaag aaaataatga ttcgatgtcg tttgcggtat cgttagattt atacccctat
     4741 gtcaaaatcg gtaagttgaa aacagtgcaa ctgacgcggc ataccgccgc catcgacgat
     4801 agcgatgtga atgaagaaat taaaaaacgt ctcatgcgtt ttgctacgta cgaagataca
     4861 gaaaatgcca ccgctgcggc gaacgacaaa gtaacagtgg attttgaaat ctgggtggac
     4921 gatgcgcctt caggtgaagt gaataaagat tttcaatttg acttaggatc aggttcgctc
     4981 agccgcgaac tcgagcagca aattatcgac aagaaaggta aggttggcga agaattcaaa
     5041 ctcaaaaaag aaattcctca gcaacaggaa gagaaaaacc gtagttacga aattattgct
     5101 accttgaaaa aaatatctaa gccaaaactc cccgaactca cagacgcgct tgtcgctgaa
     5161 aattttaagg gttcgcaaac cgttgctgaa ttcaaaaaca aagtcaaaga ggacttagaa
     5221 cgtcaatttg acttagctca aatccgtgta gaaacggaca aagcgttgaa tacactacaa
     5281 gaaaattctg aattttttat ttcagaaagc tttttagaga cccaactgga tgaatttgcc
     5341 gccgaacgca aaatacaaaa agacgaactg cgtccagagc aagccagtgg cctaaaaaaa
     5401 gcattgggcg aaagagagca ctctgcgcta ctcatgcgcc aactcctgcg cgacgcggag
     5461 aaaagccacc agaagaaaac aaagtcggaa gaaacctaca tgcaggcgtt tagaaaatac
     5521 gtgcaccagg aaattatccg gtttgaaaac gatgaacaaa aagcgtcaga gattttagtc
     5581 gatgtggatc gtgcgctaac ggctttggaa gaaggccgta gcgaaaagaa tccttgggaa
     5641 tccattatct catcgtattt aatcgttttt aaccggcagt ggatcttcga tttttttgaa
     5701 gaagaaggtc tcgttaagaa agggaagaag ctcacctata aagaacttgc agccctgtta
     5761 gggtcaactt ccagtggtcc cgatccgcta tgaaaagtat aaaactttcg acaattttag
     5821 ttttcttttt atctttcttt atagcgccct ttttacacgc cgacatcggc actcgctcca
     5881 gttggcagct catgaccgag cgcgccgaag agctgatgca agaaggcgaa gtcgaaatcg
     5941 acaaccgcct cattcaaacg taccaaggct acgccggcgt gtggacgcgc gcctcgggta
     6001 gctcgacggt aaagcgtgtt tgcaaagaag cgatgtgcga cgacgaattg ttccgccgcg
     6061 tgaacgaact caccgagtcg caaaagtcac tcaaacgcga caaactttat tttgtcacct
     6121 tcactcccga atacgtagaa aagctgcgca ccgaaggggt ttatcgccaa agagtgactc
     6181 ttaaaaaggg acagtatatt tggccggtga tgggaatccg catcactagc cgcgttggcg
     6241 gccgctgggg acgcatccac ggcggtatcg atgttgccgc tgcgcgcggt agcgtcgtcg
     6301 tcgctgccac tgacggtacg gtgatgattg ccggcgatca aggtgcgtat ggccactgtg
     6361 tgtttgtcga aaacaccgac ggcacctatg cgtggtacgc acacttaacc gattcgtatg
     6421 taaaaccagg ggataaaatt acacgcggcc aatttatcgc cgcttcggga aacaccggac
     6481 gctcgacagg accgcatctc cactttgaaa tgcgcacgca gcaagggatt atcttagacc
     6541 ccgagcattt ttttgtttta ccttttgacg aacacttaaa acaagcgcaa gattttgaat
     6601 ccgataccgc tagccatggc gcgttgacac gcttcaagca accagcacag tgatgctcga
     6661 cgtaaactat cttacacacc gcccccgccg caaccgtgta aattctgctg cgcgcgcgtt
     6721 ggtcgaagaa aaccaaatcc gcgtcgataa actcatcgaa ccactttttc tgctcgacgg
     6781 tcaaaacaaa cgcgactcca tttcttcgat gccgggaatt tttcgtttaa gcgaagacaa
     6841 cgcactccgc cacgtagaag cgtcgttgcg cttgggtatt cgtagcttta ttctttttcc
     6901 ggttatagaa gataacctca aagataacgt cgggagttac tcttacgcaa aaaataattt
     6961 ttatttgcgc attatcgcag cgctcaaaaa aaattttcat gaagcgctcc ttatctccga
     7021 tgccgcgctt gacccctgct ccaccgac
//
LOCUS       DAOMPD010000067         7021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4610_length_7021_cov_2.496985, whole genome shotgun sequence.
ACCESSION   DAOMPD010000067 DAOMPD010000000
VERSION     DAOMPD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4610_length_7021_cov_2.496985"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1063)
                     /locus_tag="PLY93_03565"
                     /pseudo
     CDS             complement(1..1063)
                     /locus_tag="PLY93_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
     gene            1563..2627
                     /locus_tag="PLY93_03570"
     CDS             1563..2627
                     /locus_tag="PLY93_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRP68587.1"
                     /translation="MARHRNPLRKEILHNRLNRKDLLKKLANEKPIPRQTFSFYRYVS
                     IATPHELRDELYREWSLLGVLGRIYIAREGINAQLAVPLENIIEFQKHLQSRKEFENM
                     RFNPGVEESEKSFLKLTIKVKRYIVNDGLGDFVLDEETAVPHLPPLEFNKALAEPETI
                     VVDMRNHYESEVGRFENALCPDVPTFREELPLVAEMLKDKKEEKILIYCTGGIRCEKA
                     GAYLKKQGYKNIFQLEGGVINYVHAVKKAKLPSKFKGKNFVFDERLAERVTDDVLTHC
                     HLCGTPCDIQINCANEGCHVLMVACETCQQKWGGCCSEECHQISLLPEEERRELRRKN
                     PKPGDAWFSKKIRVKHSPQT"
     gene            complement(2631..>2832)
                     /locus_tag="PLY93_03575"
     CDS             complement(2631..>2832)
                     /locus_tag="PLY93_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68588.1"
                     /translation="SVHWKKQKNENQLHASLSTEFLENRFIRIGMKIWVRRFRPNEEV
                     QEEIRLFIASFTRALLSDLARY"
     assembly_gap    2833..2851
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2852..4063)
                     /locus_tag="PLY93_03580"
     CDS             complement(<2852..4063)
                     /locus_tag="PLY93_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68589.1"
                     /translation="MRIKLFILFILTPPLFASYTFKDAILSLEAQNQGAKTARSLVEL
                     ANRMSEHLTYDMDTKKFFNFMNGALEDFDFARIYESAYLQSDVDGFKSLALAGQSIGG
                     GYRVSIQEKMPPQKLGNSLVRYRMRQNRQKNQTTYTTQVIAQLFTGSLSGRDLMRFLV
                     SLLKVYDPQNIESLKTAPLSIFPELQEKSRVVLDQAQADLPQTTTLFSKFISLSSLAS
                     IQTQNQKKYTAIQLRGYFRMEALKNTYPQFYQFLKRWRNLFVGEVYISDVQGQNIASL
                     IFNSNTQEFYVAFKTAQGKLLPTRKDGSVRFEDAIALDANSTQKFFIAARASINVYGL
                     KIDTGYISLFTRYILKPHELSVRTKVTHIPQGKIKGALLGVVPTWMIDLSIPSNLEEL
                     MNRFSQTLFQAN"
     gene            4153..>4552
                     /locus_tag="PLY93_03585"
     CDS             4153..>4552
                     /locus_tag="PLY93_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HRP68590.1"
                     /translation="MVAKPQIKIRATYMRGGTSKGVFFNRADLPQSAQNPGPERDALL
                     LRVIGSPDPYEKHTDGMGGATSSTSKTVIVAPSTVEGHDVDYLFGQVAIDKAFIDWSG
                     NCGNLSAAVGPFAISEGFIAKEKIPQNGICT"
     assembly_gap    4553..4562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4563..5327
                     /gene="prpF"
                     /locus_tag="PLY93_03590"
     CDS             <4563..5327
                     /gene="prpF"
                     /locus_tag="PLY93_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HRP68591.1"
                     /translation="IWQANIKKTIIAHVPITKGTVQETGDFELDGVTFPAAEIQLEFM
                     DPADGEGAMFPTGNVVDNLEVPGVGNFKTTLINAGIATIFLNAEDLHYKGTELQTDIN
                     SDAKALAMFEKIRAYGAVKMGLIKNIEEAQGRQHTPKIAFVGKPLDYKASSGKDIKAA
                     DIDLNVRALSMGKLHHAMMGTCAVAIGTAAAVPGTLVNIAAGGKEREVVTFGHPSGTL
                     RVGAKAQKINGEWVVTKAIMSRSARVLMEGAVRVPA"
     gene            complement(5346..>5671)
                     /locus_tag="PLY93_03595"
     CDS             complement(5346..>5671)
                     /locus_tag="PLY93_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68592.1"
                     /translation="TPAGLKHYLVRLDGETLVDKQYGSDEVFWRGFVEVRENEIYAIV
                     NKNGAYYLGKFDKDLKLLQQSDVKVYEDTFFSFYEDTIYVNGSDRKIEVLNRADLKAV
                     GNIVP"
     assembly_gap    5672..5705
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5706..6913)
                     /locus_tag="PLY93_03600"
     CDS             complement(<5706..6913)
                     /locus_tag="PLY93_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HRP68593.1"
                     /translation="MFKKYLLMVVVSVLLPVALFSINVNEKEIKSQAGFQFINSRDTR
                     GSDTVTAIKGIGRSLATTGSSGAYRVIHAISKEGEKFDADIIVISKNARVNHIKNIRR
                     IIEGYLVEKYKYSQADAQSLALFITYYNGFYRTNLDYFKQNYKPVVIKNLTAQNVGLD
                     KKYTGWPGASRIVIPLSEGKTPDAGKIGEKEVMEEVRKSEKTTKDRENITKLQEKEVA
                     QKEEKIQKKKEEVVKRKEEVAKREEKIKEEKQKNETNPNAADKKKKEEEIAKKEEEVK
                     KEKEEVKKQEEKVAKEEKKVEEKKTEIAQNKQNIQADKKTQTKAEERKNETPAQREAR
                     LDAKEKELKENRKEDNIFGGKLYYLRVRDYSSDGKYRNDFYLIDPKRRKVRVKSPYNG
                     IVGKKFDVVD"
BASE COUNT         1752 a         1615 c         1642 g         1949 t
ORIGIN      
        1 tgattttgag tccttgcttt ttgaggtgcg ccgctacact ctgcgctaaa cgatgtacgc
       61 gcgttgcaat ccggctaaga cctttgggcc catggtacac cgcatacatg cccgccatga
      121 ccgcaagcaa cacttgcgct gtgcagatat tcgaggtggc tttttcgcgt ttgatgtgct
      181 gttcgcgcgt ttgcaacgcc atccgcagcg ccggatcgcc ctggctgtcg accgagacac
      241 cgatgaggcg gccgggcatc tgccgtttga attcatcgcg cgtggcaaaa tacgccgcgt
      301 gtgggccgcc ataacccatc ggcacgccga aacgctgcgt cgagcccagc gcgacatctg
      361 ccccccactc gcccggaggc gtcagaaggc aaagcgaaag caaatcggcg gcaaccgcga
      421 cgagcacgcc cgatgcttta gcctgggcga caaattctgt atagtcgatg acgctaccgt
      481 cggcggctgg gtattgcacg agagcgccga aaaatgtttt atccagtgga attttgcgca
      541 cgtcgcccaa aaccagctcg acgccgatgg gtgcagagcg cgtcttcaag acgtcgatgg
      601 tttgtgggaa agtattttca gaaacaaaaa atttttttgc gtcggcattt tgcgtcaccg
      661 ctttcagcat gtgcatcgcc tcggcggcgg cggttgcctc atcgagcaaa ctcgcattgg
      721 cgatttccat accggtaagg ctcgtcacca tcgtctggta gttcagcaat gcctcaagcc
      781 gtccttgtgc aatttcggct tggtaaggcg tgtatgcggt ataccaaccc gggttttcaa
      841 aaatattgcg catcacaacc aaaggtgttt gggtaccgta atacccctgc cccaagaaat
      901 tcatgaaaag cttatttttt ctggacagct cttgtgcaag cttcaagaac tccgcctcga
      961 tcatgccgtc aggtaacgct aacgcggatg gcaaccggat gtgcgccggt atcgtttcgt
     1021 cgatgagttt atctaatgaa gaaataccga tggtttgaag catcgcatcg gtatcgtgcc
     1081 gcatcgacgc attgtgtcgg tattgaaagg catccgccgt gtcggcgata gagttctgca
     1141 tagtgggaga atttttgtcg aaacgccgcg taaagaatta tgtcgtataa gaaagagcct
     1201 gtcttcctca tttctttttt aagttgccgc gcaaactcat cgttttggca ataccatatc
     1261 atgaaggaat tctggctaat cttccgtttt attgctctct ttataaacat acggtttggc
     1321 aattttactc atttctggaa tcgcactaac gcggtctaat atgactgcct ttatgacaat
     1381 aacagaacct gtgcatcaac tcgctgagac agtttttttg caaaaaaaaa tgtcttttca
     1441 tacacgctac gcgagttggc gcacaggctc aacagtactt gagtttatac agagtatttt
     1501 gattattgtt tttgctgtgc cgacattcca ctaagtttat acaacgtctg caaaaaatca
     1561 atatggctcg acatcgcaac cctctccgca aagaaatttt acacaaccgg ctcaatcgaa
     1621 aggacttgct caaaaagttg gccaacgaaa agccaatccc tcgacagacg ttttctttct
     1681 atcgttatgt ttctatcgcc accccgcatg aactgcgcga cgagctctac cgcgaatggt
     1741 cgctcttggg ggttttgggg cgtatctaca tcgcccggga aggcatcaac gcgcagttgg
     1801 ctgttcctct ggagaatata atagaatttc aaaaacatct ccagagccgc aaagaatttg
     1861 agaatatgcg ttttaacccc ggtgtcgaag aaagtgaaaa gtcatttttg aaactcacaa
     1921 tcaaagtgaa acggtatatc gtgaacgacg gcttgggtga ttttgttttg gatgaagaaa
     1981 cagctgtgcc tcatttaccg ccgctcgagt tcaacaaggc tctcgccgaa ccggagacaa
     2041 tcgtggtcga tatgcgcaac cactacgaat ccgaggtggg gcgttttgag aatgcccttt
     2101 gccccgatgt gcctacgttc cgcgaagagt tgcctctcgt tgcagaaatg ctgaaagaca
     2161 aaaaagaaga aaaaatcctc atttactgta ccggcggcat ccgctgcgaa aaagcggggg
     2221 catacttaaa aaagcaaggc tataaaaata ttttccaact cgaaggcggc gttattaact
     2281 atgttcacgc ggtgaaaaaa gcgaaactgc catcaaaatt caaaggcaaa aattttgttt
     2341 ttgacgaacg cctcgccgag cgcgtcaccg acgatgtgtt gacgcactgc catctttgtg
     2401 gcacgccttg cgacatccaa atcaactgcg caaacgaggg ctgccacgta ctcatggtgg
     2461 cgtgcgagac atgtcaacaa aaatggggtg gctgttgtag cgaagagtgc catcaaattt
     2521 cacttttacc cgaagaagaa cgccgcgaac ttcgacgaaa aaaccccaaa ccgggggatg
     2581 cgtggtttag taagaaaatc cgagtgaaac acagccccca aacatgattg tcagtagcgc
     2641 gccaagtcgc ttaacaacgc gcgtgtgaaa gacgctataa ataggcggat ttcttcttgt
     2701 acttcttcgt tcggcctaaa ccgacggacc caaattttca taccaatacg aataaaccgg
     2761 ttttctaaaa attctgtcga gagagatgcg tgtagttgat tttcattttt ctgttttttc
     2821 caatgcacag aannnnnnnn nnnnnnnnnn ngttggcttg aaacagagtt tgtgaaaaac
     2881 ggttcatgag ttcttctaag tttgagggga tagataagtc gatcatccag gtgggaacca
     2941 cccctaaaag cgcgccttta atttttccct gtgggatgtg cgttacttta gtacggacgg
     3001 acaactcatg gggtttaaga atataacgcg taaaaagcga gatgtacccc gtgtcgattt
     3061 ttaagccata gacgttgatc gacgcgcgtg cggcgataaa aaatttttgt gtcgagtttg
     3121 catccagtgc gattgcgtct tcaaaacgca cactgccgtc tttacgtgtg ggtagcaatt
     3181 ttccttgcgc tgttttaaat gcgacgtaaa actcttgcgt gttactattg aaaatgagcg
     3241 aggcaatatt ttgcccttgt acatcgctta tatagacttc accaacaaag agattccgcc
     3301 agcgtttcaa aaattgataa aactgtgggt atgtattctt taatgcctcc atgcggaaat
     3361 acccacgcag ttggatagcg gtatattttt tctgattctg ggtttggata gaggcaaggc
     3421 tgctcaaaga aataaattta gaaaaaagcg tcgtggtttg gggaaggtcc gcctgcgctt
     3481 ggtcgagcac gacacgactt ttttcttgaa gctctggaaa aatggacaat ggcgcagttt
     3541 tgagcgactc gatattctgt ggatcgtata ctttaaggag cgatacaaga aagcgcatga
     3601 ggtcgcgccc ggacaaagac cctgtgaaaa gttgagcgat gacttgggtg gtgtacgtcg
     3661 tttggttttt ttgtcggttt tgccgcatgc ggtagcgcac gagagaattc cccaattttt
     3721 gcggtggcat tttttcttgg atggatactc gataaccacc gccgatactt tgcccagcga
     3781 gcgcaagaga tttgaagcca tcgacatcgc tctgcaaata cgcactttca tagatacgcg
     3841 caaaatcaaa atcttctaaa gcgccattca taaaattaaa aaatttcttt gtgtccatgt
     3901 cgtaggtgag gtgttcgctc atgcgattgg cgagctcaac taaagagcgc gccgttttag
     3961 cgccttggtt ttgcgcttcc aaggagagaa tggcgtcctt gaaagtatag cttgcaaaaa
     4021 gcggcggggt taaaataaag agaataaaaa gttttatccg catcggtttt tttcaaacag
     4081 tagagagcgc gcggcaacct aaaaagtata tttccatttt cgcgttgact tcttttttgg
     4141 ttatgcttgg gcatggtagc aaaaccgcaa atcaaaatcc gtgcaacgta tatgcgcggc
     4201 ggcacctcta aaggtgtttt tttcaatcgg gcagatttac cccaaagcgc gcaaaaccca
     4261 ggacctgagc gcgacgcttt actcttacgc gtcatcggaa gccccgaccc ttatgaaaaa
     4321 cataccgatg gcatgggggg cgcaacatcg tccacttcca aaacggtgat tgtggcgcca
     4381 tcgactgtgg aaggccacga tgtggattat cttttcggtc aagtggctat cgacaaagca
     4441 tttatcgatt ggagcggcaa ctgtggaaat ttatcggcag cggtgggacc atttgccatc
     4501 agtgaaggtt tcattgcaaa agagaaaatc ccacaaaatg gaatttgtac cgnnnnnnnn
     4561 nnatttggca agccaacatc aagaaaacca ttattgcgca cgtccccatc acgaaaggca
     4621 cagtgcaaga aacgggagac tttgaactcg acggggtcac attccccgca gctgaaatcc
     4681 aacttgaatt tatggatccc gccgacggtg aaggcgcaat gtttccgaca ggaaatgtgg
     4741 tggacaatct tgaggttccg ggcgtgggga atttcaaaac aacgctcatc aacgcgggca
     4801 ttgcgacgat tttcttaaac gcggaagatc ttcattacaa aggcaccgaa ctccaaacgg
     4861 atatcaattc agatgcaaag gcgctcgcga tgtttgaaaa gatccgtgca tacggcgcgg
     4921 tgaagatggg tctcataaaa aatattgaag aggcccaagg ccgccagcac actcctaaaa
     4981 ttgcttttgt gggtaaacct ttggactata aagcctcgag tgggaaagac atcaaagcgg
     5041 cagatatcga tctcaacgtg cgggcgctct cgatggggaa actccaccac gcaatgatgg
     5101 gtacctgcgc cgttgcgatt ggcaccgctg ccgctgtacc ggggacttta gtgaatatag
     5161 ctgcgggggg taaagagcgc gaagtggtca cctttggtca tccgtcgggg actttgcgag
     5221 tcggtgcaaa agcccaaaaa ataaacggcg aatgggtggt tacgaaagcc atcatgagcc
     5281 gtagcgcgcg cgtactcatg gagggggcgg tgcgtgtgcc ggcgtaaagg cacacgcgtt
     5341 aaattttagg gaacgatatt ccccactgct ttgagatcgg cgcgattcaa tacttcgatt
     5401 ttacggtcgc taccattcac gtaaatggtg tcttcgtaaa acgaaaaaaa cgtgtcttca
     5461 tacactttca cgtcgctttg ttgcaatagt ttcaaatctt tatcgaactt acccaaatag
     5521 tacgcgccat tcttgttgac gatggcgtaa atttcattct cgcgcacttc gacaaaaccg
     5581 cgccagaaaa cctcgtcgct gccgtattgt ttgtcgacaa gcgtttcacc gtcgaggcgt
     5641 acgagatagt gcttcaagcc cgccggtgtt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnttatc gacaacgtcg aattttttac cgacgatgcc gttgtaaggc gacttcacgc
     5761 gcactttacg ccttttgggg tcgatgagat aaaagtcatt acggtatttc ccgtcagagg
     5821 aataatcacg cacgcgcagg tagtagagct tgccgccgaa gatattgtct tctttacgat
     5881 tttctttgag ctctttttct ttcgcgtcta aacgcgcttc gcgttgcgca ggggtttcat
     5941 ttttacgttc ttctgctttg gtttgtgttt ttttatccgc ttggatgttt tgtttatttt
     6001 gtgcaatttc agttttcttt tcttcgactt ttttttcttc tttagcgacc ttttcttctt
     6061 gctttttaac ttcttctttt tcttttttga cttcctcttc tttttttgct atttcttctt
     6121 ctttcttttt cttgtcggca gcgttaggat tggtttcgtt tttctgtttt tcttccttta
     6181 ttttctcttc tcttttagca acctcttctt tacgcttaac aacttcttct tttttctttt
     6241 gaattttttc ttctttttgg gcgacttctt tttcttgtag tttggtgatg ttctctctgt
     6301 cttttgtggt tttttcgctc ttacgcactt cttccatcac ttctttttcg ccaattttac
     6361 ctgcatcggg agttttgcct tcgctgagtg gaataacaat gcgtgacgcg cccggccaac
     6421 ccgtgtattt tttatccaac cccacatttt gcgcggtcaa atttttaatc acaacgggtt
     6481 tatagttctg cttaaagtaa tccaaattcg tgcggtaaaa accattgtag tatgtgataa
     6541 aaagcgccaa cgactgagca tccgcctgag agtatttata tttttcaact aaataacctt
     6601 cgataatgcg gcggatgttt ttaatatgat tgacccgtgc atttttacta atcacaataa
     6661 tatcggcatc gaatttttcg ccttctttgc ttatagcatg gatgacacgg tatgcacccg
     6721 acgaacccgt tgtcgccaac gagcggccaa tgcctttgat agcggtgacg gtatcgctgc
     6781 cgcgcgtatc acggctattg atgaattgaa agcccgcttg gctttttatt tctttctcat
     6841 tgacattgat ggagaaaagc gcgacgggca gtaacacact cacaaccacc ataagaaggt
     6901 atttcttgaa cataagtgat tatcggaaaa cagccaacat aaaatcaagc tcttttgaac
     6961 tactttacta tgcaacccac agagataact gacacttttg gggtcaagtt gagtgcgttt
     7021 t
//
LOCUS       DAOMPD010000068         6941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4669_length_6941_cov_2.789428, whole genome shotgun sequence.
ACCESSION   DAOMPD010000068 DAOMPD010000000
VERSION     DAOMPD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4669_length_6941_cov_2.789428"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLY93_03605"
     CDS             <1..432
                     /locus_tag="PLY93_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68594.1"
                     /translation="KLDILFVVLAVILGGLASIALANSFALLATQNRYEFGLYLVFGS
                     SMFFLWFLLAVEGALWGFLHSYLALSLAEPTLRFLQQYMTDLPWLTKLSGTDFMNLKL
                     ELQQSERFMIYTVTISISALASLIPGMIILGRRTLSLVKKD"
     gene            complement(440..937)
                     /locus_tag="PLY93_03610"
     CDS             complement(440..937)
                     /locus_tag="PLY93_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68595.1"
                     /translation="MQKATLTHHFSFPFETLVEIREGRYNHLDIWDMFSAALLIHEEQ
                     ETSIRKRQYKLIIRTALPLIAQKLLAKGERLSCEENTVLDKNKREYRSQTSLHIANSA
                     IQFHENSIYLPNGENQSKCIIEIEANIKVPAIGKLIEKLIVHEFREQSTVDMQKIQKF
                     YNDNY"
     gene            985..1485
                     /locus_tag="PLY93_03615"
     CDS             985..1485
                     /locus_tag="PLY93_03615"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HRP68596.1"
                     /translation="MRYELKQEFPYSLATVLKARTLRYEKIQDQPGLNAQELLSVEHD
                     GPVVITRRRFRLGQAIPDIVKKMVPEKMLEMIDTNYFNTESHLSKFTMHSEYAPEKVK
                     ITAVCPYLAVSPSLTKREYEVKVDVSVPLIASALAKAIAESHRNALIKDYEILLSICE
                     SLEGGD"
     gene            complement(1503..2513)
                     /locus_tag="PLY93_03620"
     CDS             complement(1503..2513)
                     /locus_tag="PLY93_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68597.1"
                     /translation="MGKKPCSVLIGNVEHPTGFIAYTLLTTGPELKIRTQLTDSPNER
                     RILVRHNKKRIVAVCRFVLRNGPTEIVEPYALEIEDDIKKTETQSLTSSKFSIANLTS
                     TREIAYVVEANRKKIMNYVTNFFESELRNLAPYYKIYFAAGESTDMRLRQLTKQPRII
                     YYSPDPPSNFKPQQYFPQDIYLAEIQRSDLKIPVDLKAELTIPILYRQKLAIGYVQIN
                     TTRFVDDATFQTVKKIAVAAEAQLRKIGLTFEEEQILPITNIDMKTIDFIIVDRLLLR
                     HFQPNLNLLFRILKHDEPLGHFAATVSQSTNLGNGKTRIVLTFQEMDAMAELNLEEAL
                     AS"
     gene            complement(2622..5723)
                     /locus_tag="PLY93_03625"
     CDS             complement(2622..5723)
                     /locus_tag="PLY93_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68598.1"
                     /translation="MPLLYPNYYFIGALLSFIFLAYVCYLLFRVQSPSRANKHLAIAM
                     LSTAAFNLPYIFAHGFYTIPTFIPRLVNYYTPLVAGLHVAVFFLLFPSERSKKAVTII
                     LWSGHLIILTAVGYLAYVMANSPLYYLFNAHLWDSDSLPAQKYVSMLIVLYFLFFISI
                     GVWRGFKEKGEDRWGIWLIVFGFMLGTLPPAVVQVLSRDGLLTRSTFMTTTVIFNIVG
                     FFIALVTYINVTRDRTNLLGRISGIALLTILLVFQAIAYFWLHEKERNFDSIMLGNAR
                     MGYMASTLPQHASAFYTYDYIKQKSDKSPLADTYTTEQKFTLDAQATYALHSIANLKM
                     QGPDLLRDALKIIETIATHSPLQAAYLSEFIASKNFTSGKDLADAIVDLRGQQNYRAA
                     KLRQMSAHEVETKLTAAIQTMDKKFPGTLRYINSLPTHTTNETKRMLMLTALTPWHYE
                     GEKLFRGNIYYKDAMASHSIAYQFVDTTHNRVVEVAYPYLLYRKAIAESAWYIVYIII
                     ATYFAMIIGFNIFFRGTLIKPIEEIMRGLTEINNDNLDAHVEIYVEDEIGFMAKSFNK
                     MARSIKAGRMRLQQYAEQLEQKVQERTQELQTTLEDVQTLKAQQDGDYFLTTLLLKPL
                     GVNEVNSERIGIESFVRQKKHFEFRHWAKDIGGDINISHIVELDSRRHIAFINADAMG
                     KSIQGAGGALVLGSVFHTIIERTQATYAMQQLSPERWLKNTFLELHRVFESFDGSMLV
                     SGFFGLIDEESGLMYHIIAEHPRTVLYRNGKAAFITNDRMLRKLGTTSIEGSLGIATT
                     QLEEGDILLLGSDGRDDIILGYDENGNRIINEDENLFLSVVEETKGDLDKIVAKLDSM
                     GEIMDDLSLMRLERFEKARAPRLAFDYAGVLESVKYDLKGGKADAAIQRINSYLKEDP
                     FYPEAIKNLAQIYYRTKNYEKAAEYAQDYLWLKPADAHFVYFASLCFRRVRNYQKSID
                     LSERLRLREVPLGKNLALLVDLHLRIGNRKRAEALLQELIELDNTFPEIDILRAKLSM
                     AAT"
     gene            5905..>6690
                     /locus_tag="PLY93_03630"
     CDS             5905..>6690
                     /locus_tag="PLY93_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP68599.1"
                     /translation="MLKNTAILSLCFLFFSYGVLAEDPGQKIFNEKCTACHTIGKGKT
                     VGPDLKGVTTRRDPQWLTRWIMEPDKALAEKDETALALLKEFNNVPMPNFGITEAQAK
                     DIIAYLGSGDAGAETKVEISWSKSFSFTDIGTAQLTALVLFLILSTVIVVVFMMIARS
                     TRQSQPTIDMKAAYKIRAKFFVIATVIVVGTLWATLSRTPYPKTKENPNEPVKVIYVT
                     AEQFNFSYSEAPILTMEDKKTVPLISALTIPPGTLVEFQVTSID"
     assembly_gap    6691..6725
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6726..6925
                     /locus_tag="PLY93_03635"
     CDS             <6726..6925
                     /locus_tag="PLY93_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68600.1"
                     /translation="AIYDSGQRIVAQTQAMPHYTNRLRVKFDKPGDYSVLCLEYCGLG
                     HHKMRSAFKVAALPAPTESAK"
BASE COUNT         1870 a         1431 c         1607 g         1998 t
ORIGIN      
        1 aaactcgata ttcttttcgt tgtgctcgcc gttattttgg gcggcttagc gtcgattgcg
       61 ttggccaatt cgttcgcctt actcgcgacg cagaatcgat acgaatttgg gctctatctt
      121 gtttttggtt cgtcgatgtt ttttttgtgg tttttgctcg ccgtcgaagg cgcgttatgg
      181 ggatttctcc attcgtattt agcgctcagt ttggcagaac ccacgttgcg ctttctgcaa
      241 caatacatga ccgacttgcc ttggcttaca aaattatctg gcaccgattt tatgaatttg
      301 aaattagagt tacagcaaag cgaacgcttt atgatttaca ctgtcacgat ttcgattagc
      361 gcacttgcat cgttgatacc cggaatgatt attttaggca gacggacact ctcgttggta
      421 aaaaaagatt aaaagtcttt cagtaattgt cgttgtaaaa tttctgaatt ttttgcatat
      481 caaccgtact ttgctcgcgg aactcgtgca cgatgagttt ctcgataagt ttaccaatgg
      541 caggcacttt gatgtttgct tcaatttcaa tgatacactt actttgattt tcaccgttgg
      601 gtaggtaaat cgagttttca tggaattgaa tcgccgaatt agcaatatgc aagcttgttt
      661 gtgaacggta ttcacgctta ttcttatcga gcacggtgtt ttcttcgcac gaaagccgtt
      721 cgccttttgc aagtagtttt tgcgctatga gcggaagcgc cgtacggata atgagtttat
      781 attgccgttt gcggatcgag gtctcttgtt cttcgtggat aaggagcgcc gctgaaaaca
      841 tatcccaaat atcgagatga ttatacctac cttcacgaat ttctactaat gtttcaaacg
      901 gaaatgaaaa atgatgcgtc agtgttgctt tctgcacagg ggtatttatg aaagacagag
      961 tgtcggtcaa aaacaaattg aacgatgcgt tatgaactga aacaggagtt tccttattcg
     1021 ttggccaccg tccttaaagc gcgtacgtta aggtatgaaa aaattcaaga tcagcctgga
     1081 ctcaatgcac aagaactttt gagcgttgaa cacgacgggc cggttgttat cacgcgccgt
     1141 cgcttccgtt tggggcaggc aatccccgac attgtgaaaa aaatggtgcc agagaaaatg
     1201 ctcgagatga tcgacactaa ttatttcaat actgaatcgc atttaagtaa gtttacaatg
     1261 cactcagagt acgctcccga aaaagtgaaa attacagcgg tgtgtcctta tcttgccgtt
     1321 tcgccttcat taactaagcg cgaatatgaa gtaaaggtcg atgtcagcgt gccgctcatt
     1381 gccagcgcgc ttgcaaaagc gatcgccgaa tcgcaccgta atgcgctaat taaagattat
     1441 gagattttat tatcgatttg tgaaagtctt gaagggggag actaatccga aactcgcaag
     1501 agttaagacg ctaacgcctc ttctaaattt aattcggcca tcgcgtccat ttcttgaaat
     1561 gttaaaacga tacgcgtctt tccattgccc aaatttgtac tttgtgaaac tgttgcggca
     1621 aaatgaccta atggctcgtc atgttttaga atgcggaaaa gcaaatttaa gttgggttga
     1681 aagtggcgta atagaagacg atctacaatg atgaaatcaa tcgtcttcat atcgatgttt
     1741 gtgatcggta aaatttgttc ctcctcaaaa gttaatccta ttttacgtag ttgtgcttct
     1801 gccgctaccg caattttttt taccgtttgg aatgtggcgt catcgacgaa acgagtggtg
     1861 tttatttgca catatccaat ggctaatttc tgtctgtaga ggatagggat tgttaactcg
     1921 gccttcaagt cgacagggat ttttaagtca ctgcgctgaa tttcagcaag ataaatatcc
     1981 tgtggaaagt attgttgtgg cttgaagttc gatggtgggt cgggactata ataaatgatg
     2041 cgcggttgct tcgttaactg ccgcaaacgc atgtcggtgg attcgccagc agcgaaataa
     2101 attttgtaat aaggcgctaa atttctaagt tcactttcaa aaaaattggt tacataattc
     2161 attatttttt ttcgattcgc ttcgacaacg tacgcgattt cacgtgtcga cgttaaattt
     2221 gcaattgaaa attttgatga tgtaagactc tgggtttctg tttttttgat gtcgtcttca
     2281 atttcaagcg cgtatggttc cacaatttca gtggggccgt tgcgtaaaac aaaacgacac
     2341 actgccacaa tgcgcttttt gttgtgacgc acgagaatac gccgctcgtt gggcgagtcg
     2401 gttagctggg tgcgtatttt taactcggga ccggtggtga ggagagtata agcaataaat
     2461 cctgtggggt gctctacatt accgataagc accgaacagg gctttttccc catgattgtg
     2521 ttgagtattt ggtggacttg acttggttgc gtaactcgca tgatagaaaa ctctgactac
     2581 ataactctac tcttattttg agatgcctcg tcaatgagct gtcaggtcgc tgccattgaa
     2641 agttttgctc gcagtatatc gatttctgga aaagtattgt ctaactcgat caattcttgc
     2701 agtaacgctt cggcgcgttt tcgatttcct atacgtaaat gaagatcgac caaaagcgct
     2761 aagttttttc caagtggcac ttcacgcaga cgtaggcgtt cagataaatc gatagatttt
     2821 tggtaattgc gcacgcggcg aaagcaaaga cttgcaaaat aaacaaagtg cgcgtcggca
     2881 ggttttaacc acaaataatc ttgcgcatac tcggctgcct tttcgtagtt ttttgtacgg
     2941 tagtaaattt gcgccaaatt ttttatcgcc tcaggataaa acggatcttc tttgagataa
     3001 ctattaatgc gctgtatcgc cgcgtcggct ttaccgcctt tcaagtcgta ctttacactc
     3061 tctaaaacgc ctgcgtaatc aaaagctaaa cggggcgctc tggctttttc aaaccgctct
     3121 aagcgcatga gagaaaggtc gtccataatt tcgcccatcg agtcgagctt agcgacaatt
     3181 ttatcgagat caccctttgt ttcttcgaca acactcaaaa ataaattttc atcttcgttg
     3241 ataatacggt ttccattttc atcgtaaccg agaataatat cgtcacgccc gtcggaaccg
     3301 agaaggagga tgtccccttc ttccagttga gtcgttgcga tgccgagaga gccttcgatg
     3361 cttgtggtgc caagctttcg tagcatacga tcatttgtga tgaatgcagc tttaccgttg
     3421 cggtataaaa ccgtacgcgg gtgctctgcg ataatgtggt acataagccc agactcttcg
     3481 tcgatgagtc caaaaaaacc agagacaagc atcgacccat caaaactttc aaagacgcgg
     3541 tgcagctcta ggaatgtatt cttgagccaa cgctcggggg atagttgttg catagcgtat
     3601 gtcgcttggg tgcgttcgat gattgtgtga aagaccgagc ccaagaccaa tgcaccgccc
     3661 gctccttgga tagatttacc cattgcgtcg gcgttgatga atgcaatgtg acgacggcta
     3721 tccaattcaa cgatgtgtga aatattgatg tctccaccga tatcctttgc ccagtgtcta
     3781 aactcaaaat gctttttctg ccgcacaaaa gattcgatgc caatgcgttc gctattgact
     3841 tcgtttactc ccaacggttt gagcagtagc gttgtcaaaa aataatcgcc gtcttgttgc
     3901 gctttgagcg tttgaacatc ttcgagcgtc gtttgtagct cctgagttct ctcttgtact
     3961 ttttgttcga gttgttcggc gtattgttgc aagcgcatac gccctgcttt aattgagcga
     4021 gccattttat taaagctttt ggccataaag ccgatttcat cttcgacata aatttctaca
     4081 tgggcatcca gattgtcgtt gttaatctcg gttaagccac gcataatttc ttcgatgggt
     4141 ttaataagtg tcccacggaa aaagatattg aaaccaataa tcatggcaaa ataagtggca
     4201 atgataatat acacaatata ccatgcactt tcggcgatcg ctttgcggta tagtagatag
     4261 gggtatgcaa cttcgacgac gcggttgtgg gtggtatcga caaactggta ggcgattgag
     4321 tggctcgcca ttgcgtcttt ataataaata ttaccacgga ataatttttc cccttcgtaa
     4381 tgccaaggcg tcaatgcggt cagcataagc atccgtttgg tttcgttggt ggtgtgcgtt
     4441 ggcagcgagt tgatataccg cagcgtcccg ggaaattttt tgtccattgt ttggatagcg
     4501 gcggttaatt ttgtctcgac ttcatgcgcg ctcatttggc gaagttttgc cgctcggtaa
     4561 ttttgttggc cacgcaaatc gacaatagca tcggccaaat ctttgcccga ggtaaaattc
     4621 tttgaagcga taaattcaga aaggtacgcc gcttgcagcg gtgaatgcgt tgcaatggtt
     4681 tcgataattt ttaatgcgtc acggagtaaa tcaggccctt gcattttaag atttgctatc
     4741 gagtgcagtg cgtacgtcgc ttgtgcgtcg agtgtaaatt tttgctcggt tgtgtacgta
     4801 tcggcaaggg gggatttatc gctcttctgt ttgatatagt cgtaagtgta gaaagcggaa
     4861 gcgtgttgag gcaaagtact tgccatataa cccatgcgtg cattgcctag cataatggaa
     4921 tcgaaattgc gttctttttc gtgcagccaa aaataggcaa tcgcttgaaa gactaaaaga
     4981 atggttaaaa gagcgatacc gctgatgcga cctaaaagat ttgtgcggtc gcgtgtgacg
     5041 ttgatgtaag tgactagtgc aataaaaaaa ccgacgatgt tgaaaattac agtcgtcgtc
     5101 atgaaggtag agcgtgtgag caatccgtcg cggcttaaca cttgcacaac tgcgggcgga
     5161 agtgtaccga gcataaatcc aaaaacgatg agccaaattc cccaacggtc ttcgcctttt
     5221 tctttgaatc cacgccaaac gccaatagaa ataaaaaata aaaaatagag cacgataagc
     5281 atgcttacat atttttgtgc aggcaacgaa tcgctatccc aaaggtgtgc gttaaaaagg
     5341 tagtagagcg gcgagttagc catcacgtat gctaagtaac ccaccgcagt gaggataatc
     5401 aaatggccag accaaagaat aattgtaaca gctttttttg aacgttcact tgggaataac
     5461 agaaagaaaa cggcgacgtg taaaccagca accagtggag tgtagtaatt gacgagacgc
     5521 gggataaacg ttggtatcgt ataaaatccg tgtgcgaaaa tatacggtaa attaaacgcc
     5581 gccgttgaga gcatggctat tgccaagtgt ttatttgccc tggaagggga ctgcactcga
     5641 aacaggagat aacaaacgta tgcaagaaaa ataaaactaa ggagtgcgcc gatgaaatag
     5701 tagttggggt agagtagcgg catattcaac gataaaaaaa ggtatgaaaa tgtaaattaa
     5761 cttccttcaa gtaaaatatt tgagcgcggt tatacttaga cattatacag agcttcaaat
     5821 gccgaaaaaa gagtctacag tctctttctc gttgacacgc tgatgccagt tttgggcttt
     5881 tgtttagaaa aaggagtgtt tagtatgtta aagaacaccg caatcttgag tctctgtttt
     5941 ttattttttt cgtatggggt gcttgcagaa gacccaggtc aaaaaatatt caatgaaaaa
     6001 tgtactgcct gtcacaccat cggtaaaggt aaaacggttg gccctgacct caaaggggtg
     6061 accacacgcc gtgatcctca atggcttaca cgctggatta tggagccaga caaagcgctc
     6121 gctgaaaagg atgagacagc gctcgctttg cttaaagaat ttaacaacgt gccaatgcct
     6181 aattttggta tcaccgaagc gcaagcaaaa gacattattg cgtacttagg ttcgggagat
     6241 gccggtgcag aaaccaaagt agaaatttcg tggagtaaat cattttcgtt tacagacatt
     6301 ggcactgcgc aactgacggc gcttgtgctt ttcctcattc tctcgacagt gattgtcgtt
     6361 gtttttatga tgattgcccg cagcacacgg caatcgcaac ccacaatcga catgaaagcc
     6421 gcgtataaaa tccgcgcgaa gtttttcgtc atcgcgacgg ttattgtcgt tggcacattg
     6481 tgggcgacac tctcgcgcac gccttacccc aagacaaaag agaatcccaa cgagcccgtg
     6541 aaagtgattt atgtcactgc cgagcaattt aattttagct atagtgaagc ccccatactc
     6601 acgatggaag ataaaaaaac agtgccgctc atcagcgcgt tgacaattcc accggggacg
     6661 ttggtggaat ttcaagttac cagcatcgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnttgct atctacgaca gcggacaacg catcgtcgca caaacgcagg cgatgccgca
     6781 ttacacaaat cggttgcgtg taaaatttga taaacccggc gactactcgg tgttgtgtct
     6841 ggaatactgc ggtttgggtc atcacaaaat gcgctcggcg ttcaaagttg ctgcgttgcc
     6901 cgcgccaacc gaatcggcta agtgacaaaa tataaggaag g
//
LOCUS       DAOMPD010000069         6934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4674_length_6934_cov_2.838058, whole genome shotgun sequence.
ACCESSION   DAOMPD010000069 DAOMPD010000000
VERSION     DAOMPD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_6934_cov_2.838058"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..3965)
                     /gene="metH"
                     /locus_tag="PLY93_03640"
     CDS             complement(147..3965)
                     /gene="metH"
                     /locus_tag="PLY93_03640"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRP68601.1"
                     /translation="MPLYSENEIREALAQRILILDGAMGTMIQRRNLTEDDYRGKIVI
                     PSELWNSIANGPHYAAEPWISIAKDFAKNTELWNSIATHKIPLKGNSDILCLTRPDVI
                     SQIHLEYMEAGADIIETNSFSSNTVSQADYELEHLVDDLNRASVVCTLKARSDFYRKQ
                     GNPTEDKNTRKIFIAGSLGPTTKTASISPDVSNPAYRAITFDRLKNTFKEQTVALLRA
                     GVDLLLPETNLDTLNVKAAIVAFEEAFAEVGFRVPVSISVTITDASGRTLSGQTAEAF
                     YYSVRHANPLSIGINCALGAKEMKPHLAELIRVSDIPISCYPNAGLPNAFGGYDETPS
                     MFASDLATFAKEGMLNIAGGCCGTTPDHIKEMVKRVAVEKPRPGVETRHASTGASLHL
                     AGLEPLNIDGSTGFLMVGERTNVTGSPKFKKLILENKYDEALEVARQQVLAGANIIDI
                     NFDEGMLDGVEAMTKFLNLIASEPDIARVPIMIDSSRWSVIEAGLKCVQGKGIVNSIS
                     LKEGEAEFLEHARIVRDHGFAMIVMAFDEKGQAANEEDKVQVCKRAYKLLTEKAGIHP
                     SDIIFDPNILTVATGIEEHNNYAVDFINATRRIKAECPGARVSGGVSNISFSFRGNNA
                     VREAMHSVFLYYAIKAGLDMGIVNAGMLAVYEEIPLELREHVEDVILNRRADATERLL
                     AIAEKFKAGGKEQVKEDLSWREASAEERIKHALVKGIDTYVNEDVEELRLRYSPTLRV
                     IEGPLMAGMKVVGELFGAGKMFLPQVVKSARVMKKAVAYLLPFMEAEKASAGSVARQP
                     KVLLATVKGDVHDIGKNIVGVVLSCNNYDVVDLGVMVPADKILSTAKEIGADVIGVSG
                     LITPSLDEMVHVAEEMQKAEFTLPLLIGGATTSPAHTAVKIVPQYKQPVVHVADASLV
                     TGVLSRLLSEEQSAQFLEELRVEQENTRQKYLATKEDRVFLSITDARARAPKLDPFTV
                     KTPNKLGITLLDKVTLDDLVPYFDWTPFFLAWELHGRYPRILDDKIVGVEARKLFDDA
                     QKILKQILKEKPFTPHGVVGLFPAKRVGDDVEIFAADDLARKGKPTHKFYFLRQQKQK
                     QNEQVYISLADYIEKEDFMGGFAVTAGPGVTEYADFYRKKNDDYTAIIVKALGDRFAE
                     SFAEYAHARVRKEIWGYAPDENLDVQGLIKEEYLGIRPAPGYPASPDHTEKRTLFNWL
                     DVEKNTGIRLTDHFAMTPPSSVSGLYIAHPQSKYMAVGKIAPDQLEEYAARKGRTAED
                     MGHWLRPYLE"
     gene            4145..4945
                     /locus_tag="PLY93_03645"
     CDS             4145..4945
                     /locus_tag="PLY93_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68602.1"
                     /translation="MKKIITLLLSVSLVGMLSAQQPAGTDPRNSQPIVVGGDTAPEST
                     STVRTISTRKAYTATTTIPETTTTVIAPDKVLVRESALSWLSLGVRVSADYHPVAENG
                     SLSCLWLYGEFYNTNWGIEMGVGKLYQPLPTSTTTYSAGERSYWTVDAIFKYYWWFAR
                     SFWIGAGLAYNAYGDGYYVSVTKGTATSDGDHYSISNRTGQFLWQAGLGVKVALGSGF
                     NVVHFDPNIRVLGGFNNDSYVNPTTNTSTTLTGIVLRLNLGFTYSFGL"
     gene            5049..5729
                     /locus_tag="PLY93_03650"
     CDS             5049..5729
                     /locus_tag="PLY93_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRP68603.1"
                     /translation="MVEPKFATLTSQSAIFEKNQAFYEKQKTAFEASPWAKNLLTAVW
                     PTGHVAPDTTHGATPLERDGAKMFVVEMPTFTVRKEQLLDLLDFTRNNSEFQYNFLLD
                     LTALDFLKSPRAEDKTENDNKRFQMVYLFRSQVPERRSTKLRICVPVDEGEEVPSLTK
                     VWVGANWPEREVFDLMGIRFTNHPNLRRIMMPDNYRGHPLRKDFPVKGIGEDYLIEDL
                     LYKRRNVD"
     gene            5719..6594
                     /locus_tag="PLY93_03655"
     CDS             5719..6594
                     /locus_tag="PLY93_03655"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRP68604.1"
                     /translation="MSTDATTPSLPTYHGKVRDVYDLGDTLLLVSSDRLSAFDVVFED
                     TIPNKGKILNTISAHWFSLLNNTPNHFISTQSDTFPPAFQTPVFQERSVLVHKTQRID
                     FECVVRAFLMGSAYKEYTATQTVADEKLPAGLTKGAKLTAPIFTPAIKNDSGHDENIS
                     FQAMREKLPQYAAALREKSLTLFQFAFQKLEKKGVYLLDTKFEFGLLNGELLLIDEIF
                     TPDSSRFVEITEYEKAMRLGQDIPTMDKQIIRDYVESLGWNKTPPAPRLPQTIIQKTV
                     EQYKKMEDIILSIQN"
     gene            complement(6578..>6934)
                     /locus_tag="PLY93_03660"
     CDS             complement(6578..>6934)
                     /locus_tag="PLY93_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68605.1"
                     /translation="VVGPTVSSELAWVGTIGVIASLFAMLLYIWVRFEWQFGVGAIVS
                     TLHDVVLMIGLYVVFGFEFNLDKFPHAHQLKCVFAFLGERQANCLSLRFEHRFFETYC
                     GCGKESFHNKPFNFEY"
BASE COUNT         1763 a         1779 c         1625 g         1767 t
ORIGIN      
        1 aaagctatcc ttcaggcggg caggtcaccg tttcttcacc ccactgagac catagagcct
       61 acaatcgata gacgaatact tcacccacta tttcagtacg agcacctgag aatactattg
      121 ccatcaacca tatctacaag aaataatcac tctaaatagg gccgcaacca atgccccata
      181 tcttccgccg tacggccttt gcgtgcggcg tactcttcga gttgatcggg tgcaatttta
      241 cccaccgcca tgtacttcga ttgtgggtgc gcgatgtaaa ggccagacac cgacgaaggt
      301 ggcgtcatcg caaaatgatc ggttaaacga ataccggtat ttttttcaac atcgagccaa
      361 ttaaagagag tacgcttctc ggtgtggtcg ggcgatgcgg ggtagccagg cgcggggcgg
      421 atacccaagt actcttcttt gatcagcccc tgcacgtcta aattttcatc aggagcataa
      481 ccccaaattt ctttgcgcac acgcgcgtgc gcatattcgg cgaacgactc tgcaaagcgg
      541 tcgcccaacg ctttgacgat aattgcagtg tagtcgtcgt ttttcttgcg gtagaaatca
      601 gcgtattcgg tgacgcctgg gcctgcggtc acggcgaagc cgcccataaa atcttctttt
      661 tcaatatagt ccgcaagaga gatatacacc tgctcgtttt gcttttgttt ttgttggcgc
      721 agaaaataaa atttgtgtgt gggttttccc ttgcgcgcta aatcatcggc ggcaaagatt
      781 tccacgtcgt cgcccacgcg cttcgcgggg aaaagtccca ccacaccatg cggcgtaaat
      841 ggtttttctt tcaaaatttg ttttaggatt ttttgcgcat cgtcaaagag tttacgcgct
      901 tcaacaccca caattttatc gtctaaaatg cgcgggtatc tgccgtggag ttcccacgcc
      961 aagaaaaacg gcgtccagtc gaaataaggc acaaggtcgt cgagggttac tttatccaaa
     1021 agagtgatgc cgagcttgtt gggagttttc actgtaaaag gatcaagctt gggcgcgcgc
     1081 gcacgcgcat cggtaatcga caagaatact cgatcttctt tggtcgctaa atatttttgg
     1141 cgtgtgtttt cttgttcgac cctaagttct tctaaaaact gagcgctttg ctcttcgctc
     1201 aaaaggcggc ttaaaacgcc ggtgacgagc gaagcgtcgg cgacgtgcac cactggctgt
     1261 ttatattgcg gtacaatttt taccgccgtg tgcgcaggcg atgtcgtcgc accaccgatc
     1321 aagagcggta gggtgaattc tgccttttgc atctcttcgg cgacgtgcac catttcgtcc
     1381 agagacggtg tgatgagccc cgaaacgccg atcacgtcgg cgccgatttc ttttgcggtg
     1441 gataagattt tatccgcagg caccatgacg cccaagtcga caacgtcgta gttgttgcag
     1501 cttaacacca cgccgacgat atttttgccg atgtcgtgga cgtcgccttt cactgttgct
     1561 aaaagcactt tgggctgccg tgcaacgctg cccgcactcg ctttttcggc ttccataaaa
     1621 ggcaataaat atgcgaccgc ttttttcatc acccgtgcag attttaccac ttgcggcaaa
     1681 aacattttac ccgcgccgaa aagttcaccg acaactttca tgccggccat aagcgggcct
     1741 tcaatcacgc gcaaggttgg cgaataacgt aaacgtaatt cttccacgtc ttcgttgacg
     1801 tatgtatcga tacctttaac gagtgcatgc ttgatgcgct cttctgccga cgcctcacgc
     1861 cagcttaaat cttctttaac ttgctctttg ccgcccgctt taaatttttc ggcgattgct
     1921 aaaaggcgtt cagtcgcgtc ggcgcggcgg tttaaaatga catcctcgac atgctcgcgc
     1981 aactcgagtg gaatttcttc gtaaacggcg agcatccccg cgttgacaat ccccatgtcg
     2041 agccctgctt taatcgcgta atataaaaat accgagtgca tcgcttcgcg cactgcgttg
     2101 ttgccacgga acgaaaacga aatgttgctc accccgcctg aaacgcgtgc acctgggcat
     2161 tcggctttga tacggcgtgt ggcgttgata aaatccaccg cataattgtt gtgttcttcg
     2221 atacctgtcg ccacggttaa aatgtttggg tcaaaaataa tatccgaagg atggatacct
     2281 gctttctcgg tgaggagttt gtacgcacgc ttacacactt gcactttatc ttcttcgttg
     2341 gctgcctgtc ctttttcatc aaacgccatc acaatcatcg caaagccatg gtcacgcacg
     2401 atgcgggcgt gctccaaaaa ttccgcttcg ccttctttga ggctaatcga attgacaatg
     2461 cctttgcctt gcacgcattt aagccccgct tcgatgaccg accaacgcga cgagtcgatc
     2521 ataatcggta ctcgcgcgat gtcgggttcg gaagcaataa ggttcaaaaa tttcgtcatc
     2581 gcctcaacgc cgtcaagcat cccttcgtcg aagttaatat cgatgatgtt cgcgcccgcg
     2641 agcacctgct gacgcgccac ctcgagtgcc tcgtcgtatt tattttccaa aattaatttt
     2701 ttgaatttag gagagccggt cacattggtg cgttcaccca ccatcaaaaa tccggtcgaa
     2761 ccgtcgatgt tcaagggttc aagaccggcc aggtgtaaag atgcgcctgt cgaagcatgg
     2821 cgggtttcta cacctggtcg tggtttttcc accgcgaccc gttttaccat ttctttaata
     2881 tggtcggggg tggtgccaca acaaccgcca gcgatgttta gcattccttc tttggcgaac
     2941 gtggctaaat ccgatgcgaa catcgacggc gtttcatcgt aaccgccgaa tgcattaggg
     3001 agtcctgcat tggggtaaca cgaaatgggg atgtcggaga cgcgtatgag ttcggccaag
     3061 tgcggcttca tctcttttgc gcccaacgcg cagttaatgc caatcgacaa gggatttgca
     3121 tgccgcactg aataatagaa cgcttcggcg gtttgccccg aaagcgtacg tcccgatgcg
     3181 tcggtaatcg tcaccgaaat ggaaaccggt acgcggaatc ccacctctgc aaacgcttct
     3241 tcaaacgcca caatcgcggc tttcacgttc agcgtgtcta aattggtttc gggtaagagt
     3301 agatccaccc ctgctctcag aagagcgact gtttgttctt tgaatgtatt tttgagtcta
     3361 tcgaaagtaa tcgcacggta agccggatta ctcacgtcgg gggaaatcga cgccgttttg
     3421 gtggtaggtc ccagcgaccc tgcaataaaa attttacgcg tgtttttgtc ttcagtggga
     3481 tttccctgtt tgcggtaaaa atcactgcgt gctttgaggg tacacaccac cgatgcacgg
     3541 ttgaggtcat ctacaaggtg ctccaactcg taatctgcct gagagacagt gttactgctg
     3601 aacgaattcg tttcgatgat gtcggcgccg gcttccatat actcgagatg aatttggcta
     3661 atcacgtcgg ggcgggtgag gcagaggatg tcggagtttc ctttgagggg gattttatgc
     3721 gtagctatgg aattccatag ctctgtgttc ttcgcaaagt ccttagctat ggaaatccac
     3781 ggctctgcgg cgtaatgggg gccgttagcg atagaattcc ataactctga agggataact
     3841 atcttaccac ggtaatcgtc ctcggttaga ttgcgccgtt ggatcatcgt gcccatcgca
     3901 ccgtcgagga ttaaaatacg ttgggcgagc gcctcgcgga tttcattttc gctataaagt
     3961 ggcatacatc gatacatata gatacatcga tgcgttgtaa agaagatagg gatatctcat
     4021 ttcgcgatta tagagttatc tttaaaaatt atccccaaaa cagttgacac cctgtacaaa
     4081 ccgcctgaaa ttcactcatc ggggggaaac tcctaatttt gcgcataatt taaagaggta
     4141 ttgaatgaaa aaaattatca ctctactcct ctcggtctca cttgttggca tgctgtctgc
     4201 acagcaacca gcgggcaccg acccgaggaa ctcacaaccc atcgtcgtgg gcggtgatac
     4261 agcacctgaa tcgacatcaa cagtgagaac aatctcaaca cgtaaagctt acacagcgac
     4321 tacgactatt cccgaaacaa caacaaccgt catcgcaccg gataaagttc tggtacggga
     4381 atcggcatta agctggttaa gcttaggtgt tcgtgtttca gcggattatc atcctgttgc
     4441 tgaaaacggc tctttgagct gcttgtggct ctatggcgaa ttctacaaca ccaattgggg
     4501 tattgaaatg ggcgtcggta aactttacca accactacca acctcaacaa caacttactc
     4561 agcaggcgaa agaagctatt ggactgttga cgccatcttc aagtactact ggtggtttgc
     4621 ccgctcattc tggattggtg ctggtttagc atacaacgcg tatggcgacg gctactatgt
     4681 ttcagtaaca aaaggtacag caacttcaga cggcgaccac tattcaatta gcaaccgcac
     4741 aggacaattc ttgtggcaag cgggcttggg tgtgaaagtg gcattgggtt caggctttaa
     4801 cgtagttcac ttcgatccaa acatccgtgt attgggtggc tttaacaacg atagctacgt
     4861 taacccaaca acaaacacgt ctacaaccct cacagggatt gtactccgct taaacctcgg
     4921 ctttacttac agcttcggtc tttaagcgta cagtattctc ttgcgctacg cgcaggagaa
     4981 attgtataac cccaacgcga gttggggttt tttattttcg gggtatatcc ataagttttg
     5041 ttggatgtat ggtcgaacct aaatttgcga cactgacaag ccaaagcgcc atctttgaaa
     5101 aaaaccaagc attttacgaa aagcaaaaga cggcattcga ggcttcgccg tgggcaaaaa
     5161 atttacttac agcggtttgg cccaccggcc acgttgcccc cgacactaca cacggtgcaa
     5221 caccgttgga gcgcgacggt gcgaaaatgt ttgtcgtcga aatgccgacg tttacggtgc
     5281 gcaaagaaca acttttggat ttgcttgatt tcacccgcaa caactccgag tttcaatata
     5341 actttttact tgatttaacc gcacttgatt ttttgaaatc ccctcgcgca gaagataaaa
     5401 ctgaaaacga caacaagcgt tttcaaatgg tatacctttt tcgttcgcaa gttcccgaac
     5461 gccgctcgac aaagctgcgc atctgcgtgc cggtcgacga gggcgaagaa gtgccgtcgc
     5521 tcacaaaggt gtgggtaggg gcaaactggc ctgaacgcga agttttcgat ctcatgggga
     5581 ttcgctttac aaaccaccct aacttgcggc gcatcatgat gcccgacaat taccgtggtc
     5641 acccactgcg caaagatttt ccagtgaagg gtatcggtga agattatctc atcgaagacc
     5701 tcctctacaa acgccgcaat gtcgactgac gcaacaacgc catctttacc cacgtaccat
     5761 ggcaaagtac gcgacgtgta cgatttaggc gacacactac tgctcgtttc ttccgatagg
     5821 ctttctgcat ttgacgtggt gtttgaagac accatcccca ataaagggaa aattttgaac
     5881 accatctcgg cgcattggtt ttcactactt aataacacac ccaatcattt tatctccacc
     5941 cagtcggata cctttccgcc cgcatttcaa actcctgtat ttcaagagcg cagtgtactt
     6001 gtgcacaaaa cccagcgcat cgattttgaa tgcgtcgtgc gcgcattcct catgggctcg
     6061 gcgtataaag aatacactgc cacccaaacc gtcgcggatg aaaaattacc cgcaggttta
     6121 accaaaggag caaaactcac tgcacctatt tttacccctg caataaaaaa cgacagcggc
     6181 cacgacgaga atatatcgtt tcaagcgatg cgcgaaaaat taccgcaata cgccgctgct
     6241 ttgcgtgaaa aaagtttaac gctctttcaa ttcgcgttcc aaaaactcga aaaaaaaggc
     6301 gtgtatttat tagataccaa atttgaattc ggattactca acggcgagct tttgctcatc
     6361 gacgaaatat tcacccccga ttcgtcgcgc ttcgttgaaa taacggagta cgaaaaagca
     6421 atgcgcttgg gacaagacat ccccacgatg gacaaacaaa ttattcgtga ttatgtagaa
     6481 agtttgggct ggaacaagac accacctgca ccccgcttgc cgcaaaccat tattcaaaaa
     6541 acggtagagc aatataaaaa aatggaagac atcattctca gtattcaaaa ttaaaaggtt
     6601 tgttatgaaa agattctttg ccacagccac agtacgtttc aaaaaatcgg tgctcgaacc
     6661 gcagggacaa gcaattcgct tgtcgctcac cgagaaaggc gaacacgcat ttaagctggt
     6721 gcgcgtgggg aaatttatcg aggttgaact cgaagccgaa caccacgtaa aggccgatca
     6781 tgagcaccac gtcgtgcagg gtcgagacga tggcgccgac gccgaactgc cattcgaacc
     6841 gcacccagat atagagcagc atggcgaaca gcgaggcgat gacgccgatc gtgccgaccc
     6901 atgccagctc cgacgacacg gtcgggccga ccac
//
LOCUS       DAOMPD010000070         6917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4689_length_6917_cov_2.950889, whole genome shotgun sequence.
ACCESSION   DAOMPD010000070 DAOMPD010000000
VERSION     DAOMPD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4689_length_6917_cov_2.950889"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLY93_03665"
     CDS             <1..423
                     /locus_tag="PLY93_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     C-terminal domain-containing protein"
                     /protein_id="HRP68606.1"
                     /translation="KDFGWSQMTLQPNVLADATPKGKEFGSVLIDARESYQHGETVVV
                     EFQSAHPRNDLLTMDNFARVERKGANGFEFVAGDRDPEITFRWVRTKGSTSKAVLTWH
                     TKDALAGEYRLVHQGVAKSFWFGKKTRFQGVSRVFRVG"
     gene            486..713
                     /locus_tag="PLY93_03670"
     CDS             486..713
                     /locus_tag="PLY93_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRP68607.1"
                     /translation="MITSAAKFKAECLSLIDHVYENHEEITVTKRGKPMARLIPIEQK
                     TPKPLFGYMKDSMTSCGDIVSPIGEEWDVEK"
     gene            719..1129
                     /locus_tag="PLY93_03675"
     CDS             719..1129
                     /locus_tag="PLY93_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP68608.1"
                     /translation="MLLLDTHAWIWLITGDAEFGKSKALQRVLNSARKGKLRVSAISL
                     WEVSMLAAKGRVQFNIPVLNWLKDALNAPGISLEPLSPEIAVESTTLPQHFHGDPADR
                     ILVATARCHNYELVTRDRKIIEYGDAGFCKVVTI"
     gene            1196..2809
                     /locus_tag="PLY93_03680"
     CDS             1196..2809
                     /locus_tag="PLY93_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRP68609.1"
                     /translation="MENETIVGMVFNDMDLINIKEASDWATHFLGKNITPSNISYLIQ
                     YGRIKKHGQNGSTLISRSDLKEYYSSYNGQRETNWRGKLGDDLNWALSFDQYKEAETT
                     KHVHRLHPYKGKFIPQLVEYFLDTHVDNFKKEVFFQRGDIILDPFCGSGTTLVQASEL
                     GLNAVGIDISEFNTLISNTKITQYDILAIEAELRAITASLKEFLYGSKTLEFEQELLK
                     ELNIFNSKFFPVPDYKYRLARGEIQERKYGKEHEETFLPIYHKLIEQYNIQLRQKENK
                     TFLDKWYTQHIRNEINFVFQKIEQVSDSKINNILKVILSRTIRSCRATTHSDLATIFE
                     PITATYYCAKHGKICKPLFSILKWWETYSKDTIQRLIEFSRLRTKTQQVCLTGDAQTL
                     NITDKLAEVSKEFSQLVKKQKIRGIFSSPPYVGLIDYHEQHAYAYDLFGLDRRDDFEI
                     GRLSKGQSKEARLNYAQSISKVLLNSAQYLVKDFDVFLVANDKYNLYPTIAETANMQI
                     VNQYKRPVLNRTEKDKGAYSEIIFHLKAK"
     gene            2810..3589
                     /locus_tag="PLY93_03685"
     CDS             2810..3589
                     /locus_tag="PLY93_03685"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HRP68610.1"
                     /translation="MPLSKNQKEEIYSILARTLRAKFKSYKPETNSMPFHTRLLGKDR
                     LALFSFIQSLNTTFGTSIFEPVAVAIAAQKFKQAEKQQTAGSQISSQAQLTIQKILDD
                     LHTGATSPNSESEIEAIRKVCQAGEIKTVKLTKVDIKLIDQKDKILLIDLKTAKPNTN
                     EFKQFKRTLLEWVAVTLCDNPKVVVNSLIAIPYNPYDPKPYSRWTMRGMLDIENEVKV
                     AEEFWDFLGGNGTYKALLDVFENVGIDLRHEIDEQFAKFRV"
     gene            3591..5438
                     /locus_tag="PLY93_03690"
     CDS             3591..5438
                     /locus_tag="PLY93_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP68611.1"
                     /translation="MKFSIMKKIYSKLFLFTPLLFVACLEPRIDVRADAERIAQLKLF
                     QNAPPAWAMSHKDMPQFTQPEQKALLTWLAEGKIELTPQEQEKDFAFVSSVEKLLTTG
                     EKNDRLKIQTDITLDDPTNLVPTQREFFWGNIAPKIVDTLDAHVKFSACAGSILTAYD
                     KNKYYILDGHHRWASCLFVRKFLGKPEEYRKVFTPFKYYEERQIYDLLRDPKLTKIKV
                     PPPLKVTAIEGNPQGVLRVLYELAKLEHGRFSPTLPTEAEHDFMSYLQQTMFLDNPLL
                     QWIYFLLILLTSFVASRLLLFILRLKISKDEEKKRSVAFFDLLLATFKKYIYSIAFLI
                     TFKLGLRFLTLHNKLEVFIQTGLMIAVVWLFTIFASRFYANAMLRWQEKLHAREGDSE
                     ITHLFPLLIRAGKLLIYFIGFLFVMDRSGYNIYSAMAGLGVGGLALAMAGREAIGHIF
                     AGISLYLDKVVKEGDYLLLPDPINTWGRVEKVGLRSTILRTKYNSLLIVPNATLANGM
                     VNNVSVGGRKRMYRSKIILDRETPFNKIEGAVAKIKSIIESASYSPQADVHFMHFDGF
                     GFYIRVQYYVEPFTEYHDTISRNNLLILQYFDEAGIKIAMNLQNVGKQK"
     gene            complement(5485..6081)
                     /locus_tag="PLY93_03695"
     CDS             complement(5485..6081)
                     /locus_tag="PLY93_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68612.1"
                     /translation="MRFFKRNLPFTWRFTSTEKSLTLERNAPVYILSHAHNTEILNTK
                     ASLLSYAKKKGHPTVCAIRFRLERHDDIAVVRQKVRLCQEIRQNLENTYNRLNLKFLC
                     RYQTITECSLSSGQGKEAALEYLRTKKIVTEKLEPVPLYRIGTFYLYPLKHQCVTPNH
                     DWYFENTMIGADDILLPYEAQEEIQIILKNIDELRFWE"
     gene            6188..>6917
                     /locus_tag="PLY93_03700"
     CDS             6188..>6917
                     /locus_tag="PLY93_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68613.1"
                     /translation="MSKQLQKMNLLTLVIPLFFLSVYSLVAQGTVKPKSGASNEKKEA
                     PKNEIGVAEKSGAKELGYLDLVYARERALKLKEMNLRDIQLLNSIGNALPEAYDKAGA
                     TKVGDEYKEGLKQIYRIDYAAAEKTLSTNRESIAKAMEKASEVYRTKTADLLNESADR
                     MADIELQAENTGSADAVRLMGVVQDNQHRLSVGYQQLTLAATAEKRRHYNEALIHYRL
                     ARLHAIYLKLAFAKDDAERKALRET"
BASE COUNT         2142 a         1501 c         1452 g         1822 t
ORIGIN      
        1 aaagattttg gttggtcaca aatgacgttg cagccgaacg tgctcgccga tgcaaccccc
       61 aagggtaaag agttcggcag tgttcttata gacgcacgcg aatcatacca acacggcgaa
      121 acagtcgtcg tcgagtttca atccgcacac ccgcgtaacg acttactcac catggacaac
      181 ttcgcgcgcg tcgagcgcaa aggcgcaaac ggctttgagt tcgtcgccgg cgaccgtgac
      241 cccgaaatca cgttccgctg ggtacgcacg aaaggcagca cctcgaaagc cgttttaaca
      301 tggcatacaa aagacgcgct tgcaggcgag taccgtttag tccaccaggg cgtggcgaag
      361 tcgttttggt tcggcaaaaa aacacgcttt caaggtgtaa gccgcgtgtt tcgggtgggg
      421 tgagttatgg cggttaagca gaaaaaattg accaaactta taactatggt tatattacta
      481 tagtcatgat tacgtctgct gcaaaattca aagctgagtg tttaagtctc atagaccatg
      541 tttacgagaa tcatgaagaa attaccgtca ctaaacgtgg caagcctatg gcgcgcctta
      601 tacccattga acagaaaaca cccaagcctt tattcggata catgaaagat tcgatgacaa
      661 gttgcggtga catcgtttca cccatcggcg aagaatggga tgttgaaaaa tagtttccat
      721 gcttttgctc gatacgcatg cgtggatttg gctcatcacc ggcgacgccg aattcggtaa
      781 atcgaaggct ttacaacgtg tactcaattc tgcccgaaaa ggcaaacttc gtgtttcggc
      841 gattagcctg tgggaggtat cgatgctcgc ggcaaaagga cgcgtgcaat tcaacatccc
      901 cgtgttaaat tggttaaaag atgccttgaa cgcgcccgga atttctcttg aaccgttatc
      961 gcctgaaatc gcagttgaaa gcactacact gccgcaacat ttccacggcg acccagccga
     1021 ccgtatttta gtcgcgacgg cgcgctgcca taactatgag cttgtcacac gcgatagaaa
     1081 aattatcgaa tatggcgatg caggattttg taaggttgtt actatctgag aaagaaccct
     1141 atttaattct tagaatgctt taacgccaac ttgcttgacg caatgaatac aataattgga
     1201 aaatgagacg attgtgggca tggtttttaa cgacatggat ttaattaata taaaagaagc
     1261 cagtgattgg gctacccact ttttgggtaa aaacatcaca ccttctaaca ttagctattt
     1321 gattcagtac ggacggataa aaaagcacgg ccaaaatggc tcaaccttaa tttcacggag
     1381 cgacttaaaa gaatactact catcgtacaa tggtcagcgt gaaacaaatt ggcgcggaaa
     1441 gttgggtgac gacttaaact gggcactatc attcgaccag tataaagaag cagagacgac
     1501 aaaacacgtc catcgtttac atccttacaa aggaaagttt attccacaac ttgttgaata
     1561 cttcttagac acccatgtcg ataattttaa gaaagaagtt ttttttcagc gcggagatat
     1621 tattctcgat cctttttgtg gcagcggcac aacactggtg caagcatccg aattgggact
     1681 taacgcggtt ggaattgaca tctctgaatt taacacgctt atttcaaata ccaaaataac
     1741 acaatacgat attttagcaa tcgaagcaga gctaagggca atcactgctt cacttaaaga
     1801 atttctgtac ggttcaaaaa ctttagaatt tgaacaagaa cttctcaagg aactcaatat
     1861 ctttaattca aaattttttc ctgtacccga ctataaatac cgacttgctc gtggcgaaat
     1921 tcaggaacga aaatacggca aagagcatga agaaactttt ttaccgattt accataaact
     1981 tatagagcaa tacaatatcc aattacgcca aaaagagaat aagacttttt tagataagtg
     2041 gtacacgcag cacattcgaa acgaaattaa ttttgttttt caaaaaattg aacaagtttc
     2101 cgacagcaaa ataaacaata tactcaaagt tattctgagc cgaacgattc gtagttgtcg
     2161 cgctactaca cactccgact tggcaacaat ctttgagcct attacagcca catattattg
     2221 cgcgaagcat ggcaaaatat gtaaaccact cttttctatt cttaaatggt gggagactta
     2281 ctcaaaagat acaatccagc gacttataga attctctcgc ctaagaacaa aaacgcagca
     2341 agtttgctta acgggtgacg cacaaacatt aaatattacc gataagctcg cagaagtgtc
     2401 aaaagaattt tctcaactgg taaaaaagca aaaaattcga ggaatttttt cttcgcctcc
     2461 ttacgttggg cttatcgatt atcacgagca gcatgcgtat gcgtacgatt tattcggttt
     2521 agaccgccgt gatgattttg aaataggacg attgtcaaaa ggacaaagca aagaagcacg
     2581 gttaaattat gcacaatcta tatctaaggt gttactcaac agcgcgcagt accttgttaa
     2641 agattttgat gtatttcttg tcgctaacga taaatataat ttatacccaa cgattgccga
     2701 aactgccaat atgcaaattg tgaatcagta taaaagaccg gtgctcaacc gtacagaaaa
     2761 agataaagga gcctattcgg aaattatttt tcatctgaag gctaaataaa tgccactaag
     2821 caaaaaccaa aaagaagaaa tttatagtat tttagcaaga acactgcgtg ctaaatttaa
     2881 atcgtataag cctgaaacga actctatgcc gtttcataca cgacttttag gtaaagaccg
     2941 gcttgcatta ttttctttta ttcaatctct caacacaaca ttcggtacaa gtatattcga
     3001 accggttgcg gtggcaatcg cggctcaaaa attcaaacaa gcagaaaaac aacaaacagc
     3061 cggctctcaa atctcatcac aggcgcagct tacaatccaa aaaatattag acgacttaca
     3121 taccggggca acttcaccga acagtgagtc tgaaattgaa gcaattagaa aggtttgtca
     3181 agcgggagaa attaaaaccg taaaattgac aaaagtcgat ataaaactca tcgatcaaaa
     3241 agataaaatc cttcttattg atttgaaaac ggcaaaacca aacaccaacg aatttaagca
     3301 attcaagcgc actcttttag agtgggtagc cgttactctc tgcgataacc ctaaagtggt
     3361 agtaaattcg ttaattgcca taccatacaa cccttacgat cccaaaccgt atagccgatg
     3421 gacaatgcgc ggcatgctcg atatagaaaa cgaagtcaaa gtggccgaag aattttggga
     3481 ttttttaggc ggtaacggta cctataaagc actgctcgat gtgtttgaaa atgtggggat
     3541 tgatttacga catgaaatcg acgaacaatt tgcaaaattt cgagtttaat atgaaattca
     3601 gtattatgaa aaaaatatat tccaaactct ttctcttcac gcccctactc tttgtggcct
     3661 gccttgagcc gcgtatcgat gtacgcgccg acgccgagcg tatcgcccag ctaaaacttt
     3721 ttcaaaatgc gccgcctgca tgggcgatgt ctcacaaaga catgccgcaa tttacacagc
     3781 ccgaacaaaa ggcgcttcta acgtggcttg ccgaaggaaa aattgaactc accccgcaag
     3841 aacaagaaaa agattttgcg ttcgtcagct ccgttgagaa attactcacc acaggagaaa
     3901 aaaacgaccg cctcaaaata caaaccgaca ttactctgga tgatcctact aatttagtcc
     3961 ccacacaacg cgagtttttt tgggggaaca ttgctcccaa aattgtagat accctcgatg
     4021 cgcatgtaaa attttcagca tgcgctggca gtattctaac ggcatacgat aaaaacaaat
     4081 actatatttt ggacggccac caccgctggg caagctgcct tttcgtacgc aaatttttag
     4141 gcaaaccaga agaatatcgt aaagtattta ccccattcaa gtattacgaa gaacgacaaa
     4201 tttatgatct acttagagat cccaagctca caaaaattaa agtccccccc ccactgaagg
     4261 tcacagcgat tgaagggaat ccccaagggg tgttgcgtgt tctctacgaa ttggcaaaat
     4321 tggagcatgg ccgttttagc ccaacgctac ccactgaagc ggagcatgat ttcatgtcgt
     4381 atctccaaca gacaatgttt ttggacaatc cccttttaca gtggatttac ttcttgctga
     4441 ttcttttaac ctcgtttgtt gcaagtcgtt tgcttctttt tattttgcgg ttaaaaataa
     4501 gtaaagacga agagaagaag cgcagtgttg cattttttga tcttcttttg gccacgttta
     4561 aaaaatatat ttattcaatc gcctttctca ttacattcaa actggggttg cgctttctaa
     4621 cgctacataa caaacttgaa gtctttatcc aaaccggcct catgattgcg gtagtgtggc
     4681 tcttcacaat ttttgcctcc cgtttttacg ccaatgcaat gttgcgctgg caagaaaaac
     4741 tacacgcgcg cgaaggcgat tccgaaatta cgcatttatt tcccttactc atccgtgcgg
     4801 gtaaactgct gatttatttt ataggttttc tttttgtcat ggaccgtagc ggttacaaca
     4861 tttactccgc catggctggt cttggtgtgg gaggtcttgc cctcgcaatg gctggccgtg
     4921 aagccatcgg ccatatcttc gcagggattt ctctctactt agataaagtc gttaaggaag
     4981 gggattacct actcctaccc gaccccatca acacttgggg gcgtgttgaa aaggtggggc
     5041 tgcgttccac gattctccgc actaaatata acagccttct cattgtacca aacgcgaccc
     5101 tcgccaacgg catggtaaac aatgtgagcg ttggcgggcg taagcgcatg taccgcagta
     5161 aaattattct cgatcgcgaa acgccgttta ataaaattga aggcgcggtt gcaaaaatca
     5221 aaagtattat tgagagtgca tcgtactcac ctcaagccga cgttcacttt atgcatttcg
     5281 acggattcgg tttttatatt cgggtgcaat actatgtcga acccttcacc gagtaccacg
     5341 acacaataag tcgaaacaac ttattgatcc tgcaatattt tgacgaggca gggattaaaa
     5401 tcgccatgaa cctacaaaac gtagggaaac aaaaataata aatgccatca cactcttcat
     5461 ttgttacgaa gtttaatcgc agcgttactc ccaaaacctt aattcatcga tatttttcaa
     5521 aataatctga atttcttctt gtgcttcata aggcaaaagt atatcatctg cgccgatcat
     5581 tgtgttttca aaataccaat cgtggttagg agtaacgcac tggtgcttca gcgggtataa
     5641 gtagaaagta ccgatgcggt atagaggaac cggttcgagt ttttcggtta caattttttt
     5701 ggttcggaga tattcaagcg ccgcctcttt cccctgaccg gaagacaatg aacattcggt
     5761 aattgtttga tagcggcaca gaaatttaag atttaagcgg ttatatgtat tctccaaatt
     5821 ctgtcggatt tcttgacaaa ggcgcacttt ttggcgcaca acggcaatgt cgtcgtgccg
     5881 ctccaagcgg aaacgtattg cacacacggt tggatgtccc ttttttttgg catacgacaa
     5941 cagcgatgcc ttcgtgttca aaatttctgt gttatgcgcg tgtgaaagaa tatacaccgg
     6001 tgcattgcgc tcaagggtga gacttttttc tgtagatgta aagcgccacg tgaagggtaa
     6061 gttacgtttg aaaaaacgca ttaacgcttt ttcattggcc gaacgttctc gttcgcgaat
     6121 gtaatccggc gtatgcgtgt cttgattttg cgattgaccg tataaagaga gagataagga
     6181 tacaactatg agtaaacagt tgcaaaagat gaatcttcta acgttggtca tcccgctttt
     6241 tttcttgagc gtatattcgt tggtagcgca gggtacggtc aagcctaaaa gcggagcaag
     6301 caatgagaaa aaagaagcgc ccaaaaatga aatcggtgtg gcggagaaaa gtggcgctaa
     6361 agaacttggt tatttagacc ttgtgtatgc gcgcgaacgc gcattaaaat taaaagaaat
     6421 gaatttgcga gatattcaac ttttaaattc gataggtaac gcgctacccg aggcgtacga
     6481 taaagcgggt gccactaaag tgggggatga atacaaagaa ggactcaaac aaatttatcg
     6541 catcgattat gccgccgcag aaaaaacact gagtacaaac cgtgaaagta ttgcaaaagc
     6601 aatggaaaaa gccagcgaag tgtaccgcac aaaaacggcg gatttactca acgaaagcgc
     6661 cgaccgcatg gccgacatcg aattgcaagc agaaaatacg gggtcagccg atgcagttcg
     6721 actcatggga gttgtgcaag acaaccagca ccgtctttcg gtggggtatc aacagctcac
     6781 tctcgcggcc actgctgaaa aaaggcgtca ttacaacgaa gcattaatac attaccgttt
     6841 ggcgcgcttg catgcgattt atttaaaatt agcgtttgca aaagacgacg ctgaaagaaa
     6901 ggcactccgc gaaacct
//
LOCUS       DAOMPD010000071         6883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4720_length_6883_cov_2.803163, whole genome shotgun sequence.
ACCESSION   DAOMPD010000071 DAOMPD010000000
VERSION     DAOMPD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_6883_cov_2.803163"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..593
                     /locus_tag="PLY93_03705"
     CDS             111..593
                     /locus_tag="PLY93_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRP68614.1"
                     /translation="MFNIEENVVYPMHGVGKVVEIATKSILDKKKKKYYTVELINTKM
                     RVFVPVENAEAIGLRHIVDKKQIPKITKLLQEEYVEQEEDWKIRYQNNLAKVKSGSIT
                     AVAEVCRNLYKRARDKELSVMERKLYESAYSLMINEVALAKGMTVEDASNMISDILSK
                     "
     gene            961..1560
                     /locus_tag="PLY93_03710"
                     /pseudo
     CDS             961..1560
                     /locus_tag="PLY93_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
     assembly_gap    1669..1735
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1736..2336
                     /locus_tag="PLY93_03715"
     CDS             <1736..2336
                     /locus_tag="PLY93_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRP68615.1"
                     /translation="VTARKLLSSLPIDVVIFVSPKNTMGTAVVNPVIEKLKVDFPKRK
                     MKFAAAGGTRFVSFCNGLHALRQFTPLERLIVHDANRPYLTPTFLERVGKHLRYLSSD
                     LPAFIPVVPVVDSVVRLDGKNIVHYENRQELARVQTPQLIHYPTFDEKFGNALGHGRL
                     AFDFTDEGSLTLSLGMSVGTFEGDLENIKITYPEDLKVV"
     gene            2403..2822
                     /locus_tag="PLY93_03720"
     CDS             2403..2822
                     /locus_tag="PLY93_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRP68616.1"
                     /translation="MIKLLFSGNTPTCIWSDIALTVARVLVGLSLAFAHGFVKFPITE
                     GFIGYIGSTGLPMPALLAWVAAFIEAGGGILLALGLLTRPAAAFIALQMLGLVLIVHR
                     NDPFKNAEPAYLFGALALTFLAMGGGRFSLDKLFRRD"
     gene            2862..4535
                     /locus_tag="PLY93_03725"
     CDS             2862..4535
                     /locus_tag="PLY93_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP68617.1"
                     /translation="MFLDEAKAHVTSGDSSVILFSGNVRDVYASHDGKSTNIFYTLAE
                     ILRHEVLGDFNTVAEYDPQGGIQFSDATQREVYFRSLSGYDAYHKTTYAQNPPKDPGP
                     AFSILDNLARLALLDNKSFAVVIHYLDHIIPTGMTNNSELRYLIIALDKWAQNPAFRK
                     GKIRFLLIAPELARLDAFIGKNAFIERVIIPRPALDERKNTLKNANLKGEAASEEGLT
                     KLAELTAGLTNRQILTVVAKGKTDELELRAIKKELISAECRGLLEIVEPKYALDAVAG
                     HDKVKEILSLTAKAIQQGKTSHLPMGFLISGPVGTGKTFITECFAHDAALPVVKFLNF
                     RSQWQGETEANLEKIFTILKSIYPVAVIIDEADAFLGNRDAQGDSGVSMRVFASLAAL
                     MSDTKYRGKIIWFLMTSRPDLLPIDMKRQGRAEEHLALFHPQSKSELEALYQVVCKKL
                     KIQPPMAEISPILDKARAYSGADIEAMLTRSALAAEVKYESALTETILRETIGNFRSP
                     EYPLAIELQTLVAVRECTHQDMIPEKWRTMKAADIEARINELLFMLRQR"
     gene            4535..6622
                     /locus_tag="PLY93_03730"
     CDS             4535..6622
                     /locus_tag="PLY93_03730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68618.1"
                     /translation="MRLFTKAYERLTYYFLLWQGFYAAPLLHILFTSVFATAGFAYGY
                     AYGHIPQMHPDLVWALLLGLVIAILVEVYLCDGWHRLWLRSFIPRVRFVNSMFERDKL
                     IEDKSIDDYKKLLDYLTIFGKRRMYFAPLHVGIVLGTVLIYDYLFLHTGHHWVFVQTA
                     AVALPIHLQFLYVTGDLHMGKYRSQVKRQLFILGYEPPNQYAISLKAKFSSIIFVIAL
                     SDFILISMLSAASPSGSHYAIGFSLFSLSLLMVLTILYFSTIFVSIKELQLAAESLQD
                     GHDPNFYSRTSDQELATLSTGFFRAAQKVLNYRRDLEQQIREATQHVRTANEVLKQKD
                     KEIQTELDFAAEIQKDIIPLSPTPWHALRFGIVYRPMHKVSGDFFNVYKKDDELYVLL
                     ADVSGHGVPAALITMAANDAFKLAIQNLESPAAIFREVNRQLSEQIKTQDYLTAFLVR
                     IDKHMHITYANASHQKALHYQYKRDIFVSYDTNGLFIGAMADAADTYEEKAARLDYGD
                     LLVLFTDGVTEQTNDAGEEFGHERLQKILLAHREEAPQEIADAVLASLIEFAGTSRVK
                     DDISMVVMAVHPQYRRFISIFNEAVITMRRRLLAQSSTLLDELWKIYPEFPALPFLSA
                     RIYYQLKEYELAEKHVRRHLEKYPDDVRATQLLAAIALKNGNTVRAQNLIERLAKNDA
                     DNPVTKYLSKKLP"
     gene            6619..>6883
                     /locus_tag="PLY93_03735"
     CDS             6619..>6883
                     /locus_tag="PLY93_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68619.1"
                     /translation="MRNFRWFAFLVPLFFLVGCIRSEKVLQVENGKVDLSASLFSENE
                     IYYLNGQWEFYPNKILSPDEIALGNAIAERTIVPAGFNVYKESM"
BASE COUNT         1834 a         1583 c         1628 g         1771 t
ORIGIN      
        1 agcatttgtc aggtatgtcc gtgggttaga caaaccagaa ctcggttttt gattgactgt
       61 acgtctcatg ttgtggaaaa ttcgtccgct accaaggggt agcttaaaaa atgttcaata
      121 ttgaggaaaa cgtcgtttat ccgatgcacg gcgtcggtaa agttgtagaa attgctacta
      181 aatcaatctt agataaaaag aagaaaaaat attacaccgt agagctcata aatacaaaaa
      241 tgcgggtatt tgtacccgtc gaaaatgccg aggcaattgg tcttcggcac attgtagata
      301 aaaaacaaat ccccaaaatt accaagcttc tgcaagaaga atacgtagaa caagaagaag
      361 attggaaaat tcgctatcaa aataacctgg caaaagtgaa gtcaggctct attaccgccg
      421 tcgccgaggt gtgccgcaac ttatataaaa gagcgcgcga taaagaatta tcggtgatgg
      481 aacgtaagct ctacgaatct gcgtattcgc taatgattaa tgaagtggcg cttgctaaag
      541 gcatgactgt cgaagatgca agcaatatga tatccgatat tctatcgaaa taagaaatcg
      601 ggcgaagttt ttctcctttt tgtagattta caattttaag gagggaaaat gaaaaattct
      661 catttactcg cagaggctga ccaaagtgcc tcaaaaaact cattgccgta cgtcttgggc
      721 gcactcgctg gtatcgtggc ggcaggctcg cttgcaccct atttgaaaga tttaccggtc
      781 tttgtagggt atatcgtctt aggcggattg acgctcgtca gtgcggtgct ctttggtctt
      841 ttttggccac aaatgaacgg aaacaatgtg caaagcgcgc ctgtaggcgc aatagctatg
      901 gaaagtcaga gtgcaaacgg cgtcgataag acggcaaata aagaagcaaa aaccgtcgtc
      961 gaggcaaaaa ccaaaatcct cgacacgtca atcatcatcg acgggcgtat catcgatatt
     1021 gccgctacgg gatttttaga gccaccgttt ctggtgccta attttgtgct aagggaaatc
     1081 caacttattt ccgattcgcc tgacgctatc aagcgtaatc gtggacggcg cggactcgat
     1141 atgctcaacc aactccaaaa gcgcagcgac ctcgaggtta aaatttcgta taaagattac
     1201 accgaaacgc gcgaggtcga ccagaagctc atccgcttgg cgaaagatac cggcggttat
     1261 ttggtgacga atgattttaa cttaaacaaa gtggccgagt tgcaaggtgt gcgcgtgctc
     1321 aacatgaaca accttgcgaa cgcattaaag ccagtggtgt tgccgggcga agagctggaa
     1381 ctacaagtca ttaaagaagg caaagacgag aaccaaggca ttgcatatct cgaagatggc
     1441 acaatggtcg tcgtcgaagg tggcggtaaa ctcatcggca aaaaagtgaa gattagcgtc
     1501 acgtcggtta tccaaaccaa cgctggcaaa atggtgttca caaaattggc gcagaaatag
     1561 ttgctcgtac aaaaggtagg cgcatgaacg cattacttct gctcatgggt ggcatcggcg
     1621 aacgcttcgg tggtaaaaca ccgaaacaat tcctcgacat tgagttcgnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtca
     1741 ccgcgcgtaa attactcagt agtttaccca tcgacgttgt gatttttgtg tcgcctaaaa
     1801 atacgatggg caccgcagtg gtcaatccgg tgattgaaaa gctcaaggtt gattttccta
     1861 aacgcaaaat gaagtttgcc gccgcagggg gaacacgctt tgtcagtttt tgcaacggtc
     1921 tccatgcgct acgacaattt acgccactcg aacggttgat cgtccacgat gcaaaccgtc
     1981 cataccttac gccaactttt ttggaacggg tgggcaagca tttgcgctat ctttcgagcg
     2041 acttacccgc gtttattcct gttgttcccg tggtcgactc agtggtgcgt ctcgatggca
     2101 aaaacatcgt ccactacgaa aacaggcaag agttggcccg ggtgcaaaca ccgcaactca
     2161 tccactaccc aacattcgat gagaaatttg gcaatgcgct ggggcatggc cgcctcgcct
     2221 ttgattttac cgacgaaggt tcgctcactc tctctttagg catgagcgtg ggcacgtttg
     2281 aaggagattt ggaaaatatt aagattacgt atcctgaaga tttgaaagtt gtctaaaact
     2341 ttatagtcgt tattgaaaaa atgactttac tcgtatccta agcaagcaat agtgaaacta
     2401 ttatgataaa attattgttt agcggtaata caccaacgtg tatatggagc gacattgcgc
     2461 taacagtggc gcgcgttctt gttggtttgt cactggcatt cgcgcacggt tttgtgaagt
     2521 ttccaataac agaaggattt atcggttata taggcagcac aggtttacca atgcctgcgc
     2581 ttttagcgtg ggtggcggcg ttcattgaag ccggtggtgg tatattgttg gcgttaggtt
     2641 tattgacgcg ccctgcagcg gcgttcattg ctcttcaaat gctgggatta gttcttatcg
     2701 tacataggaa tgaccctttc aaaaatgccg aacctgcgta cctattcggg gcattagcct
     2761 taacgttttt ggcaatgggc ggtggtcgtt tttctctcga caaacttttt cgacgtgatt
     2821 aaagcagttt tccactgtaa atctatccat agatatggct tatgttctta gacgaagcca
     2881 aggcgcatgt tacttcgggg gatagctctg ttattctttt ttcggggaac gtgcgcgatg
     2941 tgtacgcgag tcatgatggt aaatcgacca acatatttta cacgttagca gaaattttgc
     3001 gccatgaagt cctcggggat tttaacactg tcgccgagta cgatccgcaa ggcggcatcc
     3061 aattcagcga cgccacgcag cgcgaggtat actttcgttc gctctcgggt tacgacgcgt
     3121 accacaaaac cacgtacgcg caaaatccac cgaaagaccc gggtcctgct ttttctattc
     3181 tcgataattt agcgcgactc gcgcttttgg ataataaatc gttcgcggtg gtgatacact
     3241 acctcgacca catcattccc accggcatga cgaacaactc tgagttgcgc tatctcatca
     3301 ttgcgctcga taagtgggcg cagaaccccg cgttccgtaa agggaaaatc cgttttcttt
     3361 taattgcgcc cgagctcgca cgcctcgatg catttatcgg taaaaacgct ttcattgaac
     3421 gggtgataat cccacggccc gcgctcgacg aaagaaaaaa cacgctaaaa aacgcaaacc
     3481 tcaaaggcga ggcggcgagc gaagaaggtc tcacgaagtt agccgagctc accgccggat
     3541 tgaccaaccg ccaaatttta accgtcgtgg caaaaggaaa aaccgacgaa ctcgaattac
     3601 gtgcgattaa aaaagaattg atcagtgccg agtgccgtgg ccttttagaa attgtcgagc
     3661 ccaagtatgc gctcgacgca gtcgctggcc acgacaaggt aaaagaaatt ttatcgctca
     3721 cggcaaaagc gattcaacag ggcaagacat cgcacttgcc aatggggttt ttaattagcg
     3781 gccctgtcgg tactggcaaa acattcatta ctgaatgttt tgcgcatgac gctgcgctgc
     3841 cggtggtaaa gtttttgaat tttcgctcac agtggcaggg agaaaccgaa gcaaatctcg
     3901 agaaaatttt tacaatctta aagtctatat atcccgtggc agtcatcatc gatgaagcag
     3961 acgctttttt aggtaaccgc gacgcacaag gagatagtgg cgtttcgatg cgggtgtttg
     4021 cctcgctcgc cgcgctcatg agcgacacaa aatatcgtgg taaaatcatt tggttcttga
     4081 tgacatcccg ccccgactta ttgcccatcg acatgaaacg gcaaggccgc gccgaagagc
     4141 acttagcgct tttccatcca cagtctaaaa gcgagttaga ggcgttgtac caagtggtgt
     4201 gcaagaagct caaaattcaa ccaccgatgg cggagatttc tcctatcctc gacaaagcgc
     4261 gcgcgtactc gggcgcagac atcgaagcaa tgcttacccg ttccgcgctc gctgccgagg
     4321 ttaaatacga gagcgcgctc accgaaacga ttttacgcga aacgattggc aacttccgca
     4381 gccccgagta tccactcgcg attgaactcc aaacgctggt ggcggtgcgc gagtgtacgc
     4441 accaagacat gattcccgaa aagtggcgca cgatgaaagc cgccgatatt gaagcgcgca
     4501 tcaatgagct tttattcatg ctgaggcaac gctgatgcgt ctttttacga aagcgtacga
     4561 aaggctcacg tattattttc tgctctggca ggggttttac gccgcgccac ttttgcatat
     4621 tttattcacc agtgtttttg ccacggctgg ttttgcttat ggatatgcgt atggccacat
     4681 accgcagatg cacccagacc tcgtgtgggc attgttgttg ggacttgtga ttgcgatact
     4741 cgtcgaagtt tatctctgcg acgggtggca tcgtttatgg ttgcgctcgt ttataccccg
     4801 cgtgcgtttc gtcaactcga tgttcgagcg cgataaactc atcgaagata aatcgatcga
     4861 cgactacaaa aaacttttgg attatttaac catttttggg aagcgacgca tgtactttgc
     4921 cccgctgcac gtggggattg ttctcggcac cgtacttatt tatgattact tatttttaca
     4981 caccggccac cattgggtgt ttgttcaaac tgcggcagtt gctctcccca ttcacttgca
     5041 attcttgtac gtgacgggcg acttgcacat gggtaaatac cgctcgcaag tgaagcggca
     5101 actgtttatt ttggggtacg agcctcccaa ccaatatgcg atttcgctca aagcaaaatt
     5161 ctcgagtatt atttttgtca ttgcacttag cgactttatt ttgatttcaa tgttgagcgc
     5221 tgcgtcgcca tcggggtcgc attatgcgat tggattttct cttttctcgc tttcgctttt
     5281 gatggttctc actattcttt acttctctac tatttttgtc tccattaaag aattgcaact
     5341 cgcggcagaa agtctccaag acggacacga tcccaatttt tattcaagaa cgtccgacca
     5401 agaactggcg acactctcga ccggtttttt ccgcgcggcg caaaaggtat tgaactatcg
     5461 ccgcgattta gaacagcaaa tccgcgaagc aacgcagcac gtgcgtaccg cgaacgaagt
     5521 gctcaaacaa aaagataagg agatccaaac cgagctcgac tttgccgcag agattcaaaa
     5581 agacatcatc cccctttctc ccactccgtg gcatgcgctg agatttggta tcgtctacag
     5641 accgatgcac aaagtatcgg gagatttttt caatgtttat aaaaaggatg acgaactgta
     5701 cgtgttgctc gccgacgttt cggggcatgg ggtaccggcg gcgctcatca ccatggcggc
     5761 caacgacgct tttaagttgg ccatccaaaa tttagaatcg cccgccgcca tttttcgaga
     5821 agtaaatcgt caactgagcg agcaaattaa aactcaagat tatctcaccg catttttggt
     5881 gcgtatcgac aagcacatgc acatcacgta cgccaacgct tcgcatcaaa aggcgttgca
     5941 ctaccaatac aaacgcgata tttttgtctc gtacgacacc aacggccttt ttatcggtgc
     6001 gatggctgat gccgcagata cctacgaaga gaaggcggcg cggttagact acggcgactt
     6061 gctcgtgctt tttaccgacg gcgtcaccga gcaaacgaac gacgcgggtg aagaatttgg
     6121 gcacgaaaga ttgcaaaaaa tacttttagc gcatcgtgaa gaagcgccac aagaaatcgc
     6181 cgacgctgtg cttgcgtcgt tgatagagtt tgcgggcaca tcgcgcgtca aagacgacat
     6241 ctcgatggtt gttatggcag tgcatccgca ataccgccgt tttatctcta tctttaacga
     6301 agcggtgata acgatgcgcc gtcgtttgct cgcgcaatcg tcgacgcttc tcgatgagct
     6361 gtggaaaatt tatccagaat ttccagcgct gccatttttg tctgcgcgta tttattatca
     6421 actcaaggaa tatgagcttg ccgaaaagca tgtgcgtcgc catttagaaa aataccccga
     6481 cgatgtacgc gcaacccaac tcctcgcggc gattgcactt aaaaacggca acaccgtgcg
     6541 ggcgcaaaat ttaattgagc gtctcgcaaa aaatgatgcg gataatcctg tgacaaagta
     6601 tttgtcaaag aaattaccat gagaaacttt agatggtttg catttttagt tccgctcttt
     6661 tttttagtgg gatgtattcg ttcggaaaaa gtattacaag tagaaaatgg aaaagtcgat
     6721 ttatccgctt cgctattttc tgaaaatgaa atatattact taaatggtca atgggaattt
     6781 tatccaaata aaattctttc acctgatgaa attgcgttag gtaacgccat tgccgaaagg
     6841 acgattgtcc ctgcgggatt caacgtttat aaagaatcga tgc
//
LOCUS       DAOMPD010000072         6864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4742_length_6864_cov_2.853429, whole genome shotgun sequence.
ACCESSION   DAOMPD010000072 DAOMPD010000000
VERSION     DAOMPD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4742_length_6864_cov_2.853429"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLY93_03740"
     CDS             complement(<1..596)
                     /locus_tag="PLY93_03740"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP68620.1"
                     /translation="MKLTKLNIHNFRGFEQESVNLSDITVFIGENNTGKSSVLDALRL
                     CLGNQGYVDKISRFDYRLLNNKSYAGDAGEISIRVEVCEPTVDAWPDEIQQTLPDPVD
                     LSADGRRNFFMNLKGSFDKTTDRSTEVREFQTANGVSKGAKANTSKNFLDFRNLLPVF
                     YISSLRDASKEFQQKRGLFRSFLENETIPPAKRTGIEQ"
     gene            complement(630..3596)
                     /locus_tag="PLY93_03745"
     CDS             complement(630..3596)
                     /locus_tag="PLY93_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRP68621.1"
                     /translation="MKVPEIIEQKVEDFSRNEKSLLDTKFGETETRNRFIDPFFKALG
                     WELEQTHLPRKLWDVHREYSQKDNSSTKKPDYAFRLQGKLKFFVEAKAPWVRIIDNPD
                     KDTMFQAKRYAYSTNGQAPIVIITDFQEFRVFNALQKPLYDNPNQGLLKEFDVKYKDY
                     VTKWDLIYENFSKEAVQAGSIEKLRGKISKNTKTLDAEFLGEIIKWRETLAKNIAIRN
                     EKLTVDEINEAVQRILDRLVFIRNLEDREIISENSLLKTATSKENTYKNLLPLFSELN
                     VNYNGLLFKPHFSEELDVDDKVLKDTIQAMCYPQSPFQFDVIEPEILGRIYEKFLGSK
                     IRLTDSHRAKVEEKEEVRHAGGVYYTPQFIVDYIVKDTVGKRIEGLKPEEIEKIKILD
                     PACGSGSFLLGAYSYLMEYHIAFYAKNQNVKKYKDDFFITDTNDIKLTIKKKGEILVN
                     NIWGVDIDREATEVSILSLYLKLLEHGIEDDQGWLFLKGKVLPDMTSNIRHGNSLITR
                     EDLFKNDMFGTSEIKKFDWRSETDGFGKIFVTNGGFDCIIGNPPYIRIQEMQKWAAEE
                     VELYKKIYQSGAKGNFDIYVLFIEKALELASEKGRMGFILPHKFFNASYGEQLRELLS
                     KGRNVAEVIHFGDLQVFDGATTYTCLLFLTKAPTNEIAVSKVGSLEDWVATKAAETGN
                     IPADSLTAKEWNFSIGKNAALLEKIAHIKTTLEDVTDRIFQGLKTGADKIYILEEKKR
                     SGKAIHAFSRHTGNEYKLEPDLVHPLMKGGDSKRYSLNRDTGLVILFPYAKQADGKVG
                     LIPESEMKEKFPATYEYLKECKTYLENREDGKMKGKNWFAFSRSQALDVMPQVKIFTP
                     DIAPIASYTIDDTGDMFFTGGAAGGYGILAKEHINPLYLLGLLNSSLLDFCNKQVATQ
                     MRGGWYSFEARFIKSLPIYVPDKADAEKYAICEKIPEIVQQVIDLRKAGKAGDAEFLE
                     KKIDEMVELIYEVQSR"
     gene            3830..3964
                     /locus_tag="PLY93_03750"
     CDS             3830..3964
                     /locus_tag="PLY93_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68622.1"
                     /translation="MAYKYDVAALKADLEMLLGSPWYQATKLEVKQIVEANLRQGGSL
                     "
     gene            4024..6423
                     /gene="lon"
                     /locus_tag="PLY93_03755"
     CDS             4024..6423
                     /gene="lon"
                     /locus_tag="PLY93_03755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002724703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRP68623.1"
                     /translation="MANKKESLIVPINQLLPSELFILPVRHRPVFPGMVTPLVIAEGK
                     LADAIENVYKASGHLGLVLQHDDTQEEVGPDHLFRIGTAAKILKRINLPDGGIHLLVN
                     SLERFRIDEYVHTAPHLLAKVTYEIEATEVRDKELIALMRSVLIQAKEMMAENPYFTE
                     EMKLTLINVEEPGKIADFVCSVLNLKREEFQEILETFDVRRRLERTLQFLARELDLVK
                     LQRKIQGKVSQSAETNQREFYLREQLKAIKNELRGPETKEKDADYYRQKIEAANLPNE
                     ANEKALEECAKLEYIDPIAHEYTIVRNYLDTILNLPWNIEAKDDINIIHARRVLNHDH
                     FGLNDVKTRILEYLAVRQLKKDVKGSIICLVGPPGVGKTSLGRSIANAMKKTFFRFSL
                     GGMRDEAEIKGHRRTYIGAMPGKIVQALKTTKARDMVLMLDEIDKLGRSYHGDPASAL
                     LEVLDPQQNFEFRDHFLDIPFDLSQVTFITTANTLESIPEPLIDRMEIIRLPGYILEE
                     KVQIAKHYLVNRAITDSGLTLKNAPTISSEGLKFIIDAYAREAGVRHLEKEIQKLYRK
                     AAMALVEKKSFPRTLAPKEIQTYLGKPIFTRDDEQLELAIGTAIGLAYTAMGGATLAI
                     ESREFPGRGRVKFTGQIGKVMRESSEIAVSYARSIEPNDQFWTRHDIHIHVPDGATPK
                     DGPSAGVTITTALLSLLRHKKIRTGYAMTGEIRLTGDVLPIGGLREKIVAARRMGIKH
                     VIFPHANLRDFEELPQELKKGLHAHAVKHYNEIDALLFGKVSPKKTSAATKKKIAKSK
                     K"
     gene            6433..>6864
                     /locus_tag="PLY93_03760"
     CDS             6433..>6864
                     /locus_tag="PLY93_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRP68624.1"
                     /translation="MDWSKISIAPMMEITDRRFRFFMRLLNAQVRLYTEMIVAHAIVR
                     GNRNLLLKHDASEHPVTLQLGGDDAQVLSEAAQIGEDFGYDALDLNCGCPSSRVESGH
                     FGVTLMRNPEHVAEIVARMRTRVKIPVSVKCRIGITGEASEE"
BASE COUNT         1897 a         1585 c         1459 g         1923 t
ORIGIN      
        1 ttttgctcaa tacccgtgcg ctttgccggc ggaatggttt cattttccag aaaactgcga
       61 aatagccctc ttttttgctg aaattccttt gaagcgtcac gcaatgaact aatataaaat
      121 actggtaaaa gatttctgaa atctaaaaaa tttttcgaag tatttgcctt agctccttta
      181 gagacaccat ttgccgtttg aaattctcta acttcagtag atcgatcagt ggtcttgtca
      241 aagctacctt taagattcat gaaaaaattg cgcctgccat cagcacttaa gtcgacggga
      301 tccggtaagg tttgctggat ttcatctggc catgcgtcaa ctgtaggctc acagacctca
      361 acgcgaatgg aaatttcacc tgcatcgcct gcgtatgatt tgttatttag gagtctgtaa
      421 tcaaatcgcg aaattttatc aacgtatcct tgattgccaa ggcacagcct aagcgcatct
      481 aagacactgc ttttgccggt gttattctcg ccaataaata cagttatatc ggaaagattt
      541 actgattctt gttcaaaccc gcggaaatta tgtatgttaa gcttagtgag cttcatcgtg
      601 gtaagccgtg acctttggat acgggggcgt catcgtgatt gcacctcata tattagttct
      661 accatctcat caatcttttt ctctaaaaat tccgcgtcac ccgccttgcc agccttgcgt
      721 aaatcaatga cttgttgaac aatttcggga attttctcac aaattgcgta tttttcagca
      781 tcagctttgt ccggtacgta aatcggtaga gatttaatga atcttgcctc gaagctgtac
      841 cagccgcccc gcatctgggt cgcaacctgt ttgttacaaa agtccaaaag gctactattc
      901 aataaaccta ataaatacag gggatttatg tgttctttgg caagaatacc atagccaccg
      961 gcagcaccgc cagtgaaaaa catgtcgccg gtatcatcaa tcgtataaga tgcaatgggc
     1021 gcaatgtctg gagtgaagat ttttacctgg ggcattacat ccaatgcctg agatcgacta
     1081 aaggcaaacc aattttttcc tttcatcttg ccatcttcac gattttcaag ataagttttg
     1141 cattctttta ggtactcata ggttgcagga aatttttctt tcatttcaga ttctggaatc
     1201 aagcctactt tgccatctgc ctgttttgca taaggaaaga gtatcacaag gccagtatcg
     1261 cgattcaatg agtagcgttt tgagtcaccg cctttcatca gggggtgaac caaatcaggt
     1321 tctaatttat actcgttgcc tgtgtgcctt gaaaaggcgt ggattgcttt gccactgcgc
     1381 ttcttttctt ccagaatata tatcttatca gcccctgttt tcaatccttg aaagattctg
     1441 tcagtcacat cttcgagagt ggtcttgata tgcgcaattt tttcgagcaa agcagcattc
     1501 ttgccgattg aaaaattcca ttctttagct gtcagagaat cggctggaat attgccagtc
     1561 tctgctgcct tagttgccac ccagtcttcg agcgagccaa cttttgaaac agctatttca
     1621 ttcgttgggg cttttgtcag gaagagcaag cacgtataag ttgtggcccc atcaaacacc
     1681 tgcaaatcac caaaatggat gacttcagct acgttacgtc cttttgagag caattcacgt
     1741 aattgctcac cgtagcttgc attaaagaat ttgtgcggca aaataaagcc catgcggcct
     1801 ttctcagacg ccaattcgag agccttttcg atgaataaga cataaatatc gaaattaccc
     1861 tttgctcccg actgataaat tttcttgtag agctctacct cttcggctgc ccatttttgc
     1921 atttcctgaa ttcgaatgta aggcggattc ccaatgatgc aatcgaagcc gccatttgtc
     1981 acaaagattt taccgaagcc atcagtctca ctccgccaat caaacttctt gatttccgaa
     2041 gtgccgaaca tatcgttctt aaataaatcc tccctcgtga taagtgagtt gccatgccga
     2101 atgtttgaag tcatgtcagg caagacttta cctttcagga aaagccagcc ttgatcgtct
     2161 tcaatgccat gttcaaggag tttaagatat aggctgagaa tggagacttc agtggcttcg
     2221 cggtcaatat cgactcccca gatattattt acgaggattt cacccttctt tttaatggtg
     2281 agcttgatat cattggtgtc cgtaataaaa aaatcgtcct tgtatttctt cacattctga
     2341 tttttcgcat agaatgcgat atggtattcc atgagatagg agtatgcgcc gagcagaaaa
     2401 ctaccagagc cgcaggcagg gtctaaaatt tttatctttt caatttcttc cggttttagg
     2461 ccttctattc gtttgcccac ggtatcctta acgatgtaat cgactatgaa ctgtggcgtg
     2521 taataaacac cgcctgcatg gcgaacttct tccttctctt cgactttggc cctatggcta
     2581 tcggttagac gaatcttaga acccaagaat ttttcataaa ttcggcctag gatttccggt
     2641 tctataacgt caaattgaaa cggagattgg ggataacaca ttgcctgaat cgtatccttc
     2701 agaactttgt cgtccacatc caattcttca ctaaaatgag gcttaaacaa gaggccattg
     2761 taattcacgt tcagttctga aaagaggggc aggagatttt tatatgtatt ttccttgctt
     2821 gtcgcagtct tcaatagcga attctctgaa atgatttcac ggtcctcaag atttcgaata
     2881 aatacaaggc ggtcgagaat tctctgtacc gcctcgttga tctcgtcgac ggttagtttt
     2941 tcattgcgga tagcaatgtt cttggcgaga gtctcccgcc atttgatgat ctcacccaga
     3001 aattctgcat caagggtctt ggtgtttttt gaaattttac cacgcagctt ttctatacta
     3061 cccgcttgca cggcttcttt cgaaaagttt tcataaatga gatcccattt tgtaacgtag
     3121 tctttgtatt ttacatcaaa ttccttcaat aagccttgat ttggattgtc atacaacggt
     3181 ttttgcaaag cgttgaaaac acgaaattct tgaaaatctg tgataataac gatgggtgcc
     3241 tgaccgttag tggagtaagc atatcgctta gcctggaaca tcgtgtcttt gtctgggtta
     3301 tcgataatgc gaacccacgg tgccttggct tcaacaaaga acttcagctt tccttgaagt
     3361 cggaaagcat aatcaggctt cttagtcgat gagttgtcct tttgagaata ctcgcgatga
     3421 acatcccaca atttacgcgg cagatgagtt tgctccaatt cccaacccaa ggccttgaaa
     3481 aatgggtcaa taaagcggtt tcgggtttcg gtttcgccga atttcgtatc gagaagactc
     3541 ttttcgtttc gtgaaaaatc ttcaactttt tgttcgatta tttctggtac tttcatggtc
     3601 agtcaaaaat tgattgcctt tgattaaaac tctgcatatc ttagctacca aattccgaaa
     3661 cgccccgcca aggatggcgg gtgttaacca tgcgggcaag acatgtcgcc taactatttt
     3721 tctctgtatt gcatttaatt gtgacagtca agatattttt agattaaaat gtaagatatt
     3781 atacgtataa tgatagttta caaaatgttt tacgtgtata ttgttgggta tggcgtataa
     3841 gtatgacgtt gctgcgttga aggcggatct ggagatgctg ttgggtagcc cgtggtacca
     3901 agcgacaaag cttgaggtaa agcaaatcgt cgaagcaaac ttgcgacaag ggggcagtct
     3961 ttaagttctt ttattttatc tttttatagc tttcgccttg tatttttaat aaattataac
     4021 cttgtggcca ataaaaaaga atctttaatc gtccccatta atcaattact cccctccgaa
     4081 ctttttattt taccggtgcg gcataggccg gtttttccgg gcatggtcac gccactcgtt
     4141 atcgccgaag gtaagctcgc cgacgcaatt gaaaatgtct acaaggcaag cggccacctg
     4201 gggctcgttt tacagcacga cgatacacaa gaagaagtcg gccctgatca tctgtttcgt
     4261 atcggtacgg cggcaaagat tctcaaaaga attaacttac ccgacggtgg aattcacttg
     4321 cttgtcaatt cactcgagcg ctttcgcatc gacgaatatg tgcacacagc tccgcacctt
     4381 ctcgccaaag ttacatacga aattgaagca accgaggtac gcgataaaga gttgatcgca
     4441 ctcatgcgta gtgtactcat ccaagctaaa gaaatgatgg cggagaaccc ctacttcacc
     4501 gaagagatga agctcactct catcaacgtc gaagagcccg gaaagattgc cgactttgtg
     4561 tgcagtgtac tcaacttgaa acgcgaagag ttccaagaaa ttttggaaac attcgacgta
     4621 cgccgccgat tagaacgtac tttgcaattt ctagcgcgcg aattggatct cgtaaaacta
     4681 caacgcaaaa tccaaggtaa agtgagccaa agcgctgaga caaaccaacg cgaattttat
     4741 ttacgcgaac aattaaaggc tatcaaaaac gaactaagag ggcctgagac taaagagaaa
     4801 gatgcggatt attaccgtca aaaaattgag gccgcaaact tacccaatga ggccaatgaa
     4861 aaagcgctcg aagaatgtgc aaaattagaa tacatcgacc ccattgcgca cgagtacaca
     4921 attgtgcgta actatctcga caccatttta aatttaccgt ggaatatcga agccaaagac
     4981 gatataaata taatacacgc acgacgagtc ttaaaccacg accacttcgg cttaaatgac
     5041 gtgaaaacac gcatcttaga atacctcgcc gtgcgccaac tcaaaaaaga tgtaaaaggt
     5101 tccattattt gtctcgttgg tcctcctggt gtaggcaaga cttctttggg gcgttcgatt
     5161 gcaaatgcca tgaaaaagac attcttccgt ttttctctcg gcggtatgcg cgacgaagcc
     5221 gaaattaaag gccaccgccg tacatacatc ggcgcaatgc cgggtaaaat tgtacaggcg
     5281 ttaaaaacaa caaaggcgcg cgatatggtg cttatgctcg acgaaatcga caagctcggc
     5341 cgttcatacc acggcgatcc cgcatcggcg ctcctcgaag ttttagaccc ccaacaaaat
     5401 tttgagttcc gtgatcattt tctcgacata ccgtttgatt tatcgcaagt cacctttatc
     5461 actactgcaa ataccttgga gtccataccc gagccactca tagaccgtat ggaaattatt
     5521 cgtttgcccg ggtatatcct cgaagagaaa gtacaaattg caaaacacta tctcgtgaac
     5581 cgtgccatca ccgattctgg gcttactctc aaaaatgcac cgacgatttc aagcgaagga
     5641 ttaaaattta tcattgatgc gtacgcacgc gaagcgggtg tgcggcattt agaaaaagaa
     5701 atccaaaaat tgtatagaaa agcggcaatg gcgttggttg aaaaaaaatc ttttcctaga
     5761 acactcgccc ccaaagaaat tcaaacgtat ttggggaaac ctatttttac gcgcgacgac
     5821 gaacaactcg aacttgccat tggcaccgcc atcggtttag cttacactgc aatgggtggc
     5881 gcaacgctcg cgattgaatc gcgcgaattt cctgggcgcg gacgcgtcaa gttcaccggc
     5941 caaatcggca aagtgatgcg cgagagctcg gagattgccg tctcgtatgc gcgttcaatc
     6001 gaacctaacg accaattttg gacacggcac gacatccaca tccacgtccc tgacggtgca
     6061 acccccaagg acgggccatc ggcaggcgtt acgatcacca ctgctctttt gtcgctgttg
     6121 cgtcataaaa aaattcgcac aggatacgcc atgacagggg aaatacgact aacgggcgac
     6181 gtactcccca tcggtggctt gcgcgagaaa attgtcgcag cacgacggat gggtatcaag
     6241 cacgttattt ttccgcacgc caatttgcgc gacttcgagg agctcccaca ggaattaaaa
     6301 aaaggactcc atgcgcatgc ggtgaagcac tataatgaaa ttgatgcgct tctttttggt
     6361 aaagtatccc ccaaaaaaac aagcgcagcg acaaagaaaa aaatagcaaa gtctaagaaa
     6421 taacttatga ccgtggattg gtcaaaaatc tccattgcgc cgatgatgga aatcaccgac
     6481 cgtcgctttc gctttttcat gcgccttttg aacgcgcaag tgcgtctcta taccgaaatg
     6541 attgtcgcac acgccatcgt acgcggtaac cgcaaccttc tcttgaaaca cgacgcaagc
     6601 gagcaccccg tgacgctcca actcggtggc gacgatgcgc aggtgttaag cgaagctgcg
     6661 caaattggag aagactttgg ttacgacgcc ctcgatctca actgcggctg tccatcgagt
     6721 cgtgtcgaaa gtggtcactt tggagtgaca ctcatgcgta accccgagca cgttgcggaa
     6781 attgtggcgc gtatgcgtac ccgagtcaaa atcccagtga gtgttaaatg ccgcatcggc
     6841 atcaccggtg aagcatcgga agaa
//
LOCUS       DAOMPD010000073         6854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4755_length_6854_cov_2.691425, whole genome shotgun sequence.
ACCESSION   DAOMPD010000073 DAOMPD010000000
VERSION     DAOMPD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4755_length_6854_cov_2.691425"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLY93_03765"
     CDS             complement(<1..520)
                     /locus_tag="PLY93_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68625.1"
                     /translation="MYSFGRAYFKITALLFVVFIMPLAVYGIGTTLEMPGKTAEQATE
                     KSKNVLKKLNTNCIEQDITLGSQWRCKTPFFSIVGLDIFVSTLPDRAIVRADAINRQS
                     YAFIDALAQENGEGPFTNKYEEKSLLMTIGTTLLSPALGYWYTNSNSMVRSKSMLLPV
                     LGLFLGDVALFWM"
     gene            744..>2069
                     /locus_tag="PLY93_03770"
     CDS             744..>2069
                     /locus_tag="PLY93_03770"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRP68626.1"
                     /translation="MAEYKKMYDESVKRPEAFWGRIAKQELTWFKPFKKVVKADFPKG
                     EHKWFIDGKLNLCYNALDRHLTTERKNKAALIWEPDNPATPVRTYTYQQLHYEVCRVS
                     NVLLEQGVRKGDRVTIYMPMIPELAMAVLACARIGAIHSVIFGGFSPDSIKDRVMDAK
                     SDFIITADTGYRGGKVIPIKANVDAALDKSTQEVRKVLVVRHTGDNIKFVEGRDLWWH
                     EEIEKVSADNKPAIMDAEDPLFLLYTSGSTGKPKGVLHTTGGYGVFVSFTQKIIFDLK
                     DTDTYWCTADLGWVTGHSYILYGPLMNGATSLMFEGVPTYPHVDRFWSVVEKHRVTIF
                     YTAPTAIRALMRFGEEPVAKHDLSSLRLLGSVGEPINPEAWLWYHRTIGKEKCPIVDT
                     WWQTENGGILISAIPGVTTTKPGSATLPFFGVQPVIVDGDGKELKGEAEG"
     assembly_gap    2070..2079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2080..2532
                     /locus_tag="PLY93_03775"
                     /pseudo
     CDS             2080..2532
                     /locus_tag="PLY93_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            2674..4218
                     /locus_tag="PLY93_03780"
     CDS             2674..4218
                     /locus_tag="PLY93_03780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68627.1"
                     /translation="MLRNMSLIGRLSLSFFAFTLVNLLLFWLATGSNQMRLIAEKSSL
                     EMYRTLMNIETKLTQSVRENKEMQTADFYRTEKSAEVLLNTLKNSQKGNAHTLVAFTV
                     VANDNTVYRSWPQKDAQKELSPAELQDLIKTLRLREFNNEPFYSTPNVLQYELTVYIP
                     FLNENGRDIILRAVFSMESMRQELANLFRLGVAIVLLLLLIQAGLGFILYRFIVRPLI
                     KLRAASEVIGRGENVQVKGYEKRGDEIGTLVKTFNQMVSDIQEQKSTIRKNLEEIQAR
                     DDMMQHELMIAKQIQKSIFPKGEYPHPIAIEYKPLLAVSGDFYDVYRFPDGSAGYLIC
                     DASGHGVPAALLTMLAKAAFSSAAEHIQDPGALMTQVNRQFAESLDLTGQYLTAFFMR
                     VTEKEIQYCNATHPEPLLVTTTTQRLKANGFYVGMMVDTPFPFETVVIPRVPQTKLVL
                     YTDGITEARNNEGELFGTDKLAAIIETNKDKSADAIKEIILAEVSAFTNGVAIDDDLT
                     LMVLTL"
     gene            4215..5669
                     /locus_tag="PLY93_03785"
     CDS             4215..5669
                     /locus_tag="PLY93_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68628.1"
                     /translation="MIAWVKRIRFSDLFINLVVGFGALAVAFGLMRTVTRLRMYELRD
                     SLQSFNEKNDEVRNISLLSRISLLTQSAINNNGETTARYKQEAESALITIQQARAQSE
                     QPSLVDRMALPVLNAFNFITGLPRLNLGGSSKKEMVLDLAFQFESFREYAKAVKGYSV
                     FLNDFRLTTNEREFALLHKGFSSAMLGDFASAKKDFENVVKNPLSANVEIAQKLIDFI
                     TTLDVQIKKIESVQNDALRGELYYNAAAYTKALENFARIEKNTQTEKIQFLTARSLEE
                     TGRSKEATAIYRKLIKNGSTYAVNANRRMYLLGTFLGGDKNLAQESKTNSETVVKDAE
                     FMQNFQHLEKSATELNKNAALEKERNGAQLKEITALVKVEDSPPAIKELPPVVNPTEE
                     AKPPPKPKVINLRNEKIAERAEKLDTTKKEEVLKKQPEKIDKLTMEDGNVFVGVVYKE
                     TVEQVSLYSTMGNLDLDKSKIIKREKVIGEKAFK"
     gene            5666..6415
                     /locus_tag="PLY93_03790"
     CDS             5666..6415
                     /locus_tag="PLY93_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68629.1"
                     /translation="MKKIALLFFLFTLSLVALDKGKSYDFQQKNGQNVINAQLVAEDE
                     KSYTIKLPYLAKPIQILKTNLVGEPMPSVQQAQSTFFQSLKPRFALYAGFGVAYPTLG
                     GVAYIFQRGWDARVGVDWLPFATTFWGIRALSFYTAYSFYPSMPRRIQLASAYLGPKF
                     LVRRWKNIDAALTVSFLAGTSYARLKGYTFTAEDASFSTLALVGFEKRWGRTAVGLQL
                     YVNYLFDESVTFVTTGLSISAHYPLGSAELF"
     gene            6417..6737
                     /locus_tag="PLY93_03795"
     CDS             6417..6737
                     /locus_tag="PLY93_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68630.1"
                     /translation="MFRVKDKYEDLKNLIALSFVIVLSAGILFAVLYRNIRMAVVTYE
                     IERLKKERRKIYIETEELRIKVAQHSSADRMEKLFREKYGFLPVEVSQKISTYILPRI
                     EQKK"
BASE COUNT         1934 a         1612 c         1625 g         1673 t
ORIGIN      
        1 tcatccaaaa taacgccacg tcccccagga agagtcctaa gactggcaag agcatcgact
       61 tactgcgcac catggagttt gaattggtgt accaatagcc caacgcgggt gaaaggagcg
      121 tcgtgcctat cgtcatgagt aacgacttct cttcgtattt attcgtgaat gggccttcgc
      181 cgttttcctg cgccaacgcg tcgataaacg cgtagctttg acgattgatg gcgtcggctc
      241 gtacgatggc gcggtcgggc aatgtggaaa caaaaatatc taaaccgaca attgagaaaa
      301 acggcgtttt acaccgccac tgcgatccga gggtgatgtc ttgttcgatg cagttggtgt
      361 taagcttttt aagtacgttt ttcgatttct cagtggcttg ttcggcggtt ttgcccggca
      421 tctccaacgt tgttccgatt ccatacaccg ccaaaggcat gataaagacg acaaagagta
      481 gtgcagtaat tttaaaatac gctcgcccaa atgaatacat acgcaaaaat gaataaacgt
      541 cacaaagcgt cacgcgataa gtgaaaaata cgaaactgcc ttgacgcgct ttgtggcatt
      601 acacgcattg aaatatggca gtacaaaaaa aatccccatc taaaaaaaca acatccacga
      661 aaacagcaaa aaatcccact gagcttaaat tcgaccccaa gaaaatagct cccgcgtttg
      721 taaaaaatgc gcgcatcaag aacatggccg agtacaaaaa gatgtacgac gaatcggtga
      781 aaagacctga agcgttttgg gggcgcatcg ccaaacaaga actcacttgg ttcaagcctt
      841 tcaaaaaagt cgtcaaggcg gatttcccca aaggggagca taaatggttt atcgacggta
      901 aattaaacct ctgctacaac gcgctcgatc ggcatttaac caccgaacgc aaaaataaag
      961 ccgcgttgat ttgggaacct gacaatcctg caacaccggt gcgcacttat acctaccaac
     1021 aactgcacta cgaagtgtgc cgcgtctcga acgtgctttt agagcaaggc gtgcgcaaag
     1081 gcgaccgcgt caccatttat atgccgatga ttcccgagct cgcgatggcg gttttggcgt
     1141 gcgcacgcat cggcgcaatc cactcggtca tttttggcgg attttctccc gactctatca
     1201 aagaccgcgt aatggacgcg aagtccgatt tcatcatcac cgcagacacc ggttaccgtg
     1261 gcggcaaagt aattcctatt aaagcgaacg tcgatgcggc gctcgataaa tcgacccaag
     1321 aggtgcgcaa agtgttagtg gtgcgccaca ccggcgacaa cattaagttt gtcgaaggcc
     1381 gcgatttatg gtggcacgaa gaaatcgaaa aggtgtcggc ggataacaaa cccgccatca
     1441 tggacgccga ggatccgctg tttctccttt atacatcggg ttcgacggga aaacccaaag
     1501 gcgtgttgca caccaccggt ggctatggcg tgtttgtgtc gtttacccaa aaaattattt
     1561 ttgacctcaa agacaccgat acctactggt gtactgccga cttgggttgg gtaaccggcc
     1621 acagttatat tttatacggc cctcttatga atggagccac ttcgctcatg ttcgagggag
     1681 tgcccacgta cccccacgtc gatcgctttt ggagtgttgt tgaaaaacat cgagtgacta
     1741 ttttttatac cgcgccgact gccatccgtg cgcttatgcg tttcggtgaa gaaccggtgg
     1801 caaagcacga cctctcgtcg ctgcgtcttt tgggctcggt gggtgaaccc atcaaccccg
     1861 aggcgtggct gtggtaccac cgtaccatcg gtaaagaaaa atgccccatc gtcgacacgt
     1921 ggtggcaaac cgagaatggc ggtattttaa tttctgcaat ccccggtgtc accaccacca
     1981 agcccggttc ggcgacgctg cctttctttg gtgtgcagcc agtgattgtc gacggcgatg
     2041 gcaaagagct caaaggcgaa gccgagggcn nnnnnnnnna acttgtgcat ccgctacccc
     2101 tggccgtcgc ttatgcgcgg tgtgtacggc gatcccaatc gtttttatca aacctatttt
     2161 tcgacattca aagggtatta tttctccggt gacggctgcc gtcgcgacaa agacggttac
     2221 tattggatta ccggccgcgt cgacgatgtg atcaacgtct ccggtcaccg catgggcacc
     2281 gccgaggtgg agtcggcgtt ggttctccac cctaaagtcg ctgaagccgc agtggtggga
     2341 ttcccgcacg acatcaaggg gcaaggcatc tacgcgtacg tcaccctcaa agaaggagtg
     2401 caaggaaccg aagcgttaca gcgtgaactc atccaacaag tcgcgcagga aatcggacct
     2461 atcgccaaac cagacatgat tcatttcacg ccggcgatgc cgaaaacgcg cagtggaaaa
     2521 attatgcgtc gccgacccat cggtggtgga aacattggta gccgaacgca aagtgcgggg
     2581 gtagagcatc gcacacggat ttatagacat ctgagtgcat cctgtctaaa attgtttaca
     2641 ccgacaatgg gtagctgtaa agttatataa atcatgttgc gcaacatgtc actcattggg
     2701 cggttaagcc tcagcttttt tgcgttcact ctggtgaact tattattgtt ttggttggcc
     2761 accggttcca accaaatgcg cctcattgca gagaaatcgt cgcttgaaat gtaccgcact
     2821 ttgatgaaca tcgaaacaaa attaacgcaa tcggtgcgcg aaaataaaga aatgcaaacg
     2881 gcagattttt atcgtaccga aaaatccgcc gaggtgctgt taaacacact caagaattca
     2941 caaaaaggga atgcacacac gttggtcgcg tttaccgtcg ttgctaacga caacacggtg
     3001 tatcgttcat ggccgcaaaa agacgcacaa aaggaactca gccccgctga attacaagac
     3061 ctcattaaaa ctctgcgtct gagagaattc aacaacgaac ctttttatag cacacctaac
     3121 gtgctccaat acgaactcac tgtttacatc cctttcttga atgaaaatgg ccgcgacatt
     3181 atcttgcgtg cggtgttcag catggaaagc atgcggcaag agctcgccaa tttatttcgg
     3241 ttgggtgttg ccattgtgtt gttactgctt ttaatacagg ccggtttggg atttattttg
     3301 taccggttta ttgtacgacc tctcattaaa ctccgcgcag cctctgaagt cattggccgt
     3361 ggtgaaaacg tgcaagtcaa agggtatgaa aaacgcggcg atgaaatcgg tacactcgtc
     3421 aaaacattta accaaatggt ttccgatatt caagagcaaa aatcgacgat tcgcaaaaac
     3481 ctcgaagaaa ttcaagcgcg tgatgacatg atgcagcacg agctcatgat tgctaaacaa
     3541 attcaaaaaa gtatttttcc caaaggcgaa tatccacacc ccattgcaat tgagtataaa
     3601 ccgcttttgg ccgtatcggg cgacttctac gatgtttatc gttttcccga tggcagtgcg
     3661 ggctatttaa tttgcgacgc ttcggggcat ggcgtgcccg cagcgctact cacaatgttg
     3721 gcgaaagccg ctttttcaag cgccgccgag cacatacagg acccgggcgc gctcatgacg
     3781 caggtgaatc gtcagttcgc cgaatctctc gatttaacgg ggcaatactt gaccgcgttt
     3841 tttatgcgcg tgacagaaaa agaaatccaa tactgcaacg ccacgcatcc cgaaccgcta
     3901 ctcgtgacaa ccaccacgca gcggcttaaa gcaaacggtt tctatgtggg gatgatggtc
     3961 gatacgcctt ttccttttga aacggtggtt atcccccgtg tgccgcaaac taaactcgtt
     4021 ctttataccg acggcatcac cgaggcacgt aataacgaag gggaattatt cggcactgac
     4081 aaactcgctg cgattataga aacgaacaaa gacaagtcgg cggatgcaat caaagaaatt
     4141 attttagccg aagtgtcggc attcacgaac ggcgtcgcca tcgacgacga tttaactctc
     4201 atggttttaa cgctatgatt gcgtgggtaa aaagaatacg ctttagcgat ttatttatta
     4261 atttagttgt cggcttcggt gcgctcgctg tggcgtttgg tcttatgcgc acagtcacac
     4321 ggttgcgcat gtacgagttg cgcgattcac tccagtcgtt caacgaaaag aatgatgaag
     4381 tgcgcaatat ctcgcttttg tcgcgcattt cgttgttgac ccaaagcgca ataaataaca
     4441 acggtgaaac aacagcgcgc tacaaacaag aagcggaaag tgcgttgata accattcaac
     4501 aagcgcgtgc gcagagtgag cagccttcgc ttgttgacag aatggcgtta cctgtactaa
     4561 atgcgttcaa tttcattacc ggattaccgc gtttgaattt aggagggagc tcaaaaaaag
     4621 aaatggtcct cgacttggct tttcagtttg aaagttttcg cgaatatgcg aaagcagtaa
     4681 aaggttatag cgtcttcttg aacgattttc gtctgacgac caacgagcgc gaatttgcac
     4741 tgttgcataa aggattttct tcggcgatgc tgggcgactt tgcttcagct aaaaaagatt
     4801 ttgagaacgt ggtgaaaaat ccactttcgg cgaatgtcga gattgcgcaa aagttgattg
     4861 actttatcac cacgttagac gtgcagatta aaaaaattga atctgtgcaa aacgatgcgt
     4921 tgcgaggcga actttactat aacgcagcgg cgtacacaaa ggctctcgaa aattttgcgc
     4981 gtattgaaaa aaatacgcaa accgaaaaaa tacaattttt aacagcgcgc tctttagaag
     5041 aaacagggcg ctcaaaagaa gcaacggcaa tttatcgaaa actcatcaaa aatggttcta
     5101 cgtatgcagt gaatgccaac cgtcggatgt acctcttagg tacattttta ggaggcgaca
     5161 aaaacttagc gcaggagtca aaaactaatt ctgagacggt cgtgaaagac gccgagttca
     5221 tgcaaaactt tcagcactta gagaaatcgg caacagagtt aaataaaaat gccgcgcttg
     5281 aaaaagaaag aaacggtgca caattaaaag aaataaccgc gttagttaaa gtcgaagatt
     5341 ccccccctgc tataaaagaa ctcccacccg tggtaaatcc cactgaggaa gcaaagccac
     5401 ctcccaaacc taaagtcata aacctgcgca atgaaaaaat tgccgaacgt gcagagaagc
     5461 ttgacacgac aaaaaaagaa gaggtgttaa aaaagcagcc cgagaaaatc gataaactca
     5521 cgatggaaga cggtaatgtt ttcgttggcg tggtgtataa agaaacggta gagcaagtgt
     5581 ctctttattc gacgatgggc aatctcgact tagataaatc caaaattatt aaacgtgaaa
     5641 aagttatcgg cgagaaagca tttaagtgaa aaaaattgcg ttgttatttt ttttatttac
     5701 tctatcgctt gtcgctctcg acaaagggaa atcgtacgac tttcaacaaa aaaatgggca
     5761 gaacgtgata aatgcccagc tcgttgccga agatgaaaaa tcgtatacca ttaaattgcc
     5821 ttatttagca aaaccgatac aaattttgaa aaccaatctt gtgggtgagc cgatgccctc
     5881 tgtgcaacaa gcacaatcca cgtttttcca atcgctcaaa cctcgttttg ctttatacgc
     5941 aggttttggg gtggcgtacc ccacgttggg tggcgttgcg tatatttttc aaaggggatg
     6001 ggatgcacgc gtgggtgtcg attggttgcc gtttgcaaca actttttggg gaatacgcgc
     6061 actttcgttt tatacggcat attcgtttta tccaagcatg ccgcggcgca ttcaattggc
     6121 atcggcatat ttagggccta aatttttagt ccgccgctgg aaaaatatcg acgctgcgtt
     6181 gaccgtatcg tttttagctg gcacaagtta tgcacgcctc aaaggttaca cgtttaccgc
     6241 agaggacgcc tcgtttagta cgttagcgct cgttgggttt gaaaaacgat gggggcgtac
     6301 cgcagtggga ttgcaattgt atgtaaatta tctgttcgat gaatctgtaa cttttgtaac
     6361 gaccggactg agtatttctg cccactaccc tttgggtagc gcagaacttt tttaatatgt
     6421 ttcgagttaa agataagtac gaagacttaa aaaatttaat cgcgctctcg tttgtcatcg
     6481 tgctcagcgc cggcatcttg ttcgcggtgt tgtaccgcaa catccgtatg gcggtcgtca
     6541 cgtatgaaat cgagcgcctc aaaaaagaac gccgaaaaat ttatatcgaa accgaagagt
     6601 tgcgtatcaa agtggcgcag cattcctccg ccgaccgcat ggaaaaactc tttcgcgaaa
     6661 aatatggatt tttgcccgtc gaggtgagtc aaaaaattag cacgtatatc ttaccgcgca
     6721 tcgaacaaaa aaaatgaaat ttaagttgca cgctcctttt aagcccgcgg gtgatcaacc
     6781 gcaagccatc gccgatcttg ccgaagcatt gaagccatcc gacagcaaag caatgctcat
     6841 cggcgtaacc ggct
//
LOCUS       DAOMPD010000074         6817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4783_length_6817_cov_2.566253, whole genome shotgun sequence.
ACCESSION   DAOMPD010000074 DAOMPD010000000
VERSION     DAOMPD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4783_length_6817_cov_2.566253"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..352)
                     /locus_tag="PLY93_03800"
     CDS             complement(164..352)
                     /locus_tag="PLY93_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68631.1"
                     /translation="MKSPNFFIQTTISENIFRSGGDRIVEQTLNFELRSVETQLVVFS
                     DRVIYRKKPRNQGGGVTW"
     gene            complement(405..719)
                     /locus_tag="PLY93_03805"
     CDS             complement(405..719)
                     /locus_tag="PLY93_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68632.1"
                     /translation="MREHRLILFLAILLALAACSTTTKRLDADKDITADSGELTRGEL
                     EKAADNLAQKVGAYMAANKPAAGVFVAFLPTKNDTSDQLPVNIFDNRIVDKLLAAKIY
                     AF"
     gene            822..1892
                     /locus_tag="PLY93_03810"
     CDS             822..1892
                     /locus_tag="PLY93_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68633.1"
                     /translation="MLPWFIALLVILNTALGAAYEDKALARVAILTYEDETHTNNFQY
                     MPGSLTEAIDKSLQTKFEYRREEPTKSQAARQKLKPHGLLNAQEAVLYCKNNDVQILV
                     FGRFQYDARRREIIVDTYISLGSVESFRHLKVRRNPTNATIFTLAERVADDIVAEMTL
                     IAKEQGERTSKPQTENEKITLEKETPVTWTPKLYFVTVGMGGVQPLAALKDVYPATLI
                     PSVSFNRLVWRNFYTGIFSGLFQFKGQETQNVNFRSSLTTLATTFNVGYAWFFGKDRW
                     RVNTEFGAGYYGSKFLVESESANIYSRMFSNPVLRASTSLHFLIFSALSIGVEAGYLQ
                     LVDDNATDALTAYALLNVGFVF"
     gene            1904..2569
                     /locus_tag="PLY93_03815"
     CDS             1904..2569
                     /locus_tag="PLY93_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68634.1"
                     /translation="MLKKVTFRNSVLFIFLWIFSSGCSNIGFVDDAVRVAEGKFGIEV
                     FVTGGVATGGYLATPGAYTVNCNGQTGVAKADCICQDEANNRQFTGTYRAWLSTSGVD
                     AICNIQGRREAACSAKASMGPFVMRTQNGMRILANDYSELSSVGMRIPLDPLASMVNI
                     WTGSTAAGRATMDNCNDWTMGTTSIKGTVGDKSKIGTAFTDTGAVLGCDQQGSLLCMR
                     QVE"
     gene            2587..3891
                     /locus_tag="PLY93_03820"
     CDS             2587..3891
                     /locus_tag="PLY93_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68635.1"
                     /translation="MKKHWFLILRRTVLFFALAFIPIQTDAYDENGVLMGGFTLMGND
                     GAAEAGENPAYLVFPKNEAASSLAFRSWGESALNTSFSKLNVESSLQWLAAGDLSYAL
                     HKFMDNKLAIGASLNSVRNPTIHRFDFQASTYLPALAASITSNSSMEQLQAAASVGAA
                     YRLTASESIGVQLKYSARFESNISKANFEKFSVRNLQEAHNDNTMHTATAGVSYLFRT
                     SPADFSFMVGNLGYKYQSVAFTHTASGNILGSVSEAGSFNNQFLTQPEFTLGSRVRLF
                     DAVHLFTELGAQPPIECSGSDKTYRDEAPKGVVVRSFTARFEPGFSAALGFSFVVTPT
                     LTLFTGVRGEKNQAASTFTSVSPGEYTNKNQTVLQMVASGGAAWRWNKILLQAGVQYF
                     YQAVDQSQSARLNNGIGTITKDSGFQIEINGLGGYFGVAFDF"
     gene            3972..5126
                     /gene="leuB"
                     /locus_tag="PLY93_03825"
     CDS             3972..5126
                     /gene="leuB"
                     /locus_tag="PLY93_03825"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRP68636.1"
                     /translation="MQYNIATLAGDYIGPEVMQSAIAVLSKLADDKHQFNFQEALVGG
                     AAIDATGKALPTETLKVAEGAHSILFGSVGGPKWEHLPPAEQPERAALLPLRKHFSLY
                     ANLRPAVIYKELKDASPLRADIVGDGLDILILRELTGDVYFGEPKVRRGEGPTEEALD
                     TMIYKRFEIERIAHLAFQSARTRRKTVHSIDKANVLTSMVFWREIVTEVAKNYPDVKL
                     IHMYVDNAAMQLVRAPSQFDVVLCPNMFGDILSDEASQITGSIGMLASASLGVDGEGA
                     GGQKSWGKDGLTFGLYEPAGGTAPDIAGQGIANPIAQILSAALMLRYSLKLEDKASRI
                     ENAVKKAIASGARTRDLVPSTALGARDGRGVSPTTAFSTAEMTQEIIKNL"
     gene            5158..6186
                     /gene="rlmN"
                     /locus_tag="PLY93_03830"
     CDS             5158..6186
                     /gene="rlmN"
                     /locus_tag="PLY93_03830"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRP68637.1"
                     /translation="MDILSLTEDELAQILVADGHKKFRASQVFQWLHLKQVFDVRRMT
                     NLKADFQDYLLNKFQFPDFQILNHRESPVDHTAKYLFAFSRRQVEAVWLPYENRQSLC
                     VSVQSGCSLNCSFCATGKIDFKGNLSAAEILTQVYGTQQLYDRRVTNVVFMGMGEPFY
                     NYDNVIRAASLLAHPHGMNISRRRITVSTSGVFTGIKRFVEEKQPYRLALSLHAVFPE
                     KRNQLMDIEKKFPYEEVLDYLREHKQDLQPGQLMFEYIMINDINIFPEDAKELTRWAK
                     LLGAKVNLIPMNTTFNGMTRPPQEKIDAFWKMLVKNGVVAVNRKSPAKDIDGACGMLA
                     GSLMPSAL"
     gene            complement(6167..>6817)
                     /locus_tag="PLY93_03835"
     CDS             complement(6167..>6817)
                     /locus_tag="PLY93_03835"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRP68638.1"
                     /translation="EAFGHGTEADTMQKGRAKGSDYLGKFVASSQVSLYDSPALEGAA
                     ASYYFDHEGLPASETKIIEKGILTQPMTDHTSASRLNFRRSPNGRRESFDHKVYSRMT
                     NTYFMPGDDDLATMIASVEDGYFVDRATNGMEDPKSWGIQLEALVARRIRHGKLTDEY
                     FSPVIVTGYVPEILQSISMVSHDMHINGLGMCGKGYKEWVKVTDGGPYLKLKARLA"
BASE COUNT         1683 a         1633 c         1777 g         1724 t
ORIGIN      
        1 gcagtgctgt cttgcgccaa aggggtgatt gttgaaacgg cagtgtcgat gtcgccgtgg
       61 taaaacgcat tttcggtggc ttggattttt ttagcgtacg accccgcgca tgcaacgagg
      121 gtagcgcttg taataaaaat agagaatcgc tgaaaaaggc gcgtcaccat gtcacgccgc
      181 cgccttggtt gcggggcttt ttacggtaga taacgcggtc gctgaacacg accagttgcg
      241 tttccaccga acggagttca aaatttaacg tttgctcgac aatgcgatcg ccaccgctgc
      301 ggaaaatatt ttcactgatg gtggtttgga taaagaagtt ggggcttttc atgttgcccg
      361 cagaaagccc gccggaggtg gcgccggttt gtgcaaacgc aatttcagaa agcgtaaatt
      421 ttggcggcta aaagcttatc cacaatgcgg ttgtcgaaaa tatttactgg cagttgatcc
      481 gacgtgtcgt tcttggtggg tagaaacgcg acaaagacac cggcagcggg tttatttgcc
      541 gccatgtatg cgcctacttt ttgcgcaagg ttgtcggcgg ctttctccaa ttcgccgcgc
      601 gtgagttcgc ccgaatcggc ggtgatgtct ttatcggcgt cgagacgctt ggtggtggtc
      661 gaacaggcgg cgagcgccaa gaggattgca agaaataaaa tgagacgatg ttcacgcata
      721 cacccgtgta gaattatttt ctctcttggc aagatttatt tattgtcgag acgcactaaa
      781 ttttgcttga atatttgaaa atactgacta gaatgtcgca aatgctaccg tggtttatcg
      841 cgcttcttgt tattctcaat accgcccttg gcgcggcata cgaagataaa gcgcttgcgc
      901 gcgttgcaat cttgacttac gaagatgaaa cacacacaaa taatttccag tatatgccag
      961 ggtcgcttac cgaagccatt gataaatcat tgcaaaccaa gtttgaatac agacgcgaag
     1021 aaccgacaaa gtcgcaagcc gcgcgccaaa aactaaaacc gcacggcttg cttaacgccc
     1081 aagaggcggt actctattgc aaaaataacg acgtacaaat tctggtgttt ggtcgttttc
     1141 agtacgacgc acggcggcgg gaaattatcg tcgatacgta tatctcactc ggtagtgttg
     1201 aaagctttcg gcatcttaaa gtccgccgca accccaccaa tgccacaatt ttcacgttgg
     1261 ccgaacgcgt cgccgacgat atcgtcgccg aaatgacgct cattgcaaaa gagcagggcg
     1321 aacgcacaag caaacctcaa acggagaatg aaaaaattac acttgaaaaa gaaactcccg
     1381 tcacatggac gcctaaactt tattttgtaa cggtgggtat gggtggagtg cagcctttgg
     1441 ccgcgcttaa ggatgtgtat ccggcaacgc taatcccttc agtctcgttt aatcgtctcg
     1501 tgtggcgtaa cttttatacg ggcattttct ctggactttt tcaattcaag ggccaggaaa
     1561 cacagaatgt aaattttcgc agtagcctca cgacgttagc gacgacgttt aacgttgggt
     1621 atgcgtggtt ctttgggaaa gatcgctggc gcgtgaacac agagttcgga gcggggtatt
     1681 acggtagcaa gttccttgtg gaatctgaaa gcgctaatat ttactcacgc atgttttcaa
     1741 accctgtact cagggcgtcg acgtcgctcc actttttaat tttcagcgcc ttgtcgattg
     1801 gcgttgaagc ggggtatttg caactcgtgg atgataacgc gaccgatgcc ctaacggcgt
     1861 atgcattgtt gaacgtgggg tttgtttttt aagggggatt cttatgttaa agaaagtaac
     1921 ttttcggaat tcagttcttt ttattttctt atggatattt tcctctggtt gtagcaacat
     1981 tggttttgtc gacgatgctg tgcgtgtggc ggaaggaaag ttcggcattg aagtttttgt
     2041 gaccggtgga gttgccactg gtggctacct tgctactccc ggtgcataca cggttaactg
     2101 caacggtcaa acgggtgttg ctaaagcgga ctgcatttgc caagacgaag caaacaacag
     2161 gcaatttaca ggcacttacc gcgcctggct ctcgacaagt ggcgtcgacg ccatttgcaa
     2221 catccaaggg cgcagagaag ctgcttgctc tgccaaagcg tctatggggc cttttgtaat
     2281 gcgaacccag aacggtatgc gcatcttggc gaacgattac agtgaactga gcagtgtggg
     2341 catgcgcatt ccactcgacc cacttgcgtc catggtcaat atctggacgg gtagcactgc
     2401 tgccggacgt gcgacgatgg acaattgtaa cgactggaca atgggtacaa catcaatcaa
     2461 agggactgtg ggtgataaat cgaagattgg tactgcattc accgacacgg gcgcggtgct
     2521 tggatgcgat cagcaaggta gtcttttatg catgcggcag gttgagtgat tgctatgcaa
     2581 ataagaatga agaagcactg gtttcttatt ttaaggcgca cggtactttt ttttgctttg
     2641 gcgtttatcc ccatacaaac cgacgcttac gatgaaaacg gtgtactcat gggtggattt
     2701 actctcatgg gcaacgacgg cgcggcggag gcgggagaaa atcctgcgta tttagttttt
     2761 cccaaaaacg aggccgcttc ctcgcttgcc ttccgcagtt ggggggagtc ggcgttaaac
     2821 accagtttct ctaaactgaa cgtagagagc tcgctccagt ggcttgctgc gggtgattta
     2881 tcttatgcgt tgcataagtt catggataac aaactggcca tcggtgcaag tttaaatagc
     2941 gtgcgtaatc ccacaataca ccggtttgat tttcaggcat caacctattt acctgctttg
     3001 gcggcgagta tcaccagcaa cagttcaatg gaacaactgc aagctgcggc gtctgtgggg
     3061 gctgcctatc gattgactgc gagtgaatcg attggtgtcc aattaaaata ctcagcacgc
     3121 tttgaatcca atataagtaa agctaacttt gaaaaattca gtgtgcgcaa tttacaggaa
     3181 gcgcataacg acaatacgat gcacactgcg accgcagggg tgagttatct attccgcacc
     3241 agccctgccg atttttcgtt tatggtgggt aacttggggt ataaatacca aagtgttgct
     3301 tttacacaca cagcatctgg caacattctg ggaagcgtgt ccgaggcggg ttcttttaac
     3361 aatcagtttt taactcaacc tgaatttact ttaggcagcc gtgtgcggtt attcgatgcg
     3421 gtgcatcttt ttaccgagct gggggcgcag ccgcccatcg agtgttcggg gagtgataaa
     3481 acgtaccgcg acgaggcgcc taagggtgtt gttgtgcggt cgtttacagc gcggtttgag
     3541 ccgggctttt cggcagcact gggcttttcg tttgtggtca caccaacgct gaccttattt
     3601 acaggtgtac gcggcgagaa aaatcaagcg gcatcgacct tcacttcggt aagccccggt
     3661 gaatatacga ataaaaatca aacggtgctc caaatggttg ccagtggggg agcggcatgg
     3721 cgatggaata aaattttatt acaggcaggg gtgcagtatt tttatcaagc ggtcgatcag
     3781 tctcaaagcg ctcggcttaa caatggcata ggtaccatta ctaaagattc gggttttcaa
     3841 attgaaatca acggtttggg tggttacttt ggcgtggcgt ttgatttttg aatctacccc
     3901 atacacgctg cgcgagttgg cgcacaggct ctatctcgtt tacacagcgt ctcaaatctt
     3961 gacaatgcag tatgcaatac aatatcgcaa cgttagcggg tgactatatc ggcccagagg
     4021 tgatgcaatc ggctatcgca gttctctcca aacttgccga cgataaacat cagtttaatt
     4081 ttcaagaagc gctcgtcggc ggtgcagcca tcgacgccac aggcaaagcg ctccccacag
     4141 agacgcttaa agtagccgaa ggcgcacaca gtattttgtt tggttcggtg ggcggcccga
     4201 agtgggaaca tttacccccc gccgagcagc ctgaacgagc ggcgctttta cctctccgta
     4261 aacatttttc actctacgca aatttacgcc ctgcggtgat ttataaagaa ctcaaagacg
     4321 catcgccatt acgcgccgac atcgtgggcg acgggctcga cattctcatc ttgcgtgaac
     4381 tcacgggcga cgtgtatttc ggcgaaccca aagtgcggcg cggcgaaggg ccaactgaag
     4441 aggcgctcga cacgatgatt tataaacgtt ttgagattga gcgtattgcg catctcgctt
     4501 tccagtcggc acggacgcgg cgtaaaactg tgcattcgat tgataaagcc aatgttttga
     4561 ctagcatggt tttttggcgt gaaattgtca ccgaggttgc gaaaaattac cccgatgtaa
     4621 aactcatcca catgtacgtg gacaacgcag cgatgcagct cgtgcgtgcg ccgtcgcaat
     4681 tcgacgtggt gctttgcccc aatatgttcg gcgatatttt atccgacgaa gccagccaaa
     4741 tcaccggttc gattggtatg ctcgcttcgg cgtcgttggg cgtcgatggc gagggtgcgg
     4801 gcggtcaaaa aagttgggga aaagacggct tgacgtttgg tctttacgaa ccggcaggcg
     4861 gcaccgcacc cgacatcgcc ggccaaggca tcgcaaaccc catcgcgcaa attctctcgg
     4921 ctgcgcttat gctgcgctat tcgttgaagt tagaagataa agcaagccga attgaaaacg
     4981 cagtaaagaa ggcgattgct agcggcgcac gcacacgcga tttagtcccc tcgactgcgc
     5041 tcggtgcaag agacggtcgc ggggtaagtc ccaccacagc gttttcaact gccgaaatga
     5101 ctcaggagat cataaaaaat ttatagccat tgaggcgcag tccccgcggc gactactatg
     5161 gatatactct cgctcaccga agacgaactt gcccaaatac tcgtcgctga cgggcataaa
     5221 aaatttcgag caagccaagt gtttcaatgg ctgcacttga agcaagtctt cgacgtgcgg
     5281 cgcatgacga accttaaagc cgactttcag gattatctct taaataaatt ccagtttccc
     5341 gattttcaaa ttttgaatca ccgtgaatcg ccggttgacc atacggcaaa gtatcttttt
     5401 gcgtttagcc gccgccaagt cgaagcggtg tggctaccct atgaaaaccg acagtcgctt
     5461 tgtgtttcgg tgcaatcggg ttgttcgttg aactgtagtt tttgcgctac cggaaaaatt
     5521 gactttaagg gaaatctatc cgcagccgaa attttgacgc aggtgtatgg tacgcaacaa
     5581 ctctacgacc gccgcgtcac taacgtggtg ttcatgggaa tgggcgagcc cttttacaat
     5641 tacgacaacg tcatccgcgc ggcatcactc ttagcgcacc cgcacggcat gaacatctcc
     5701 cgccgtcgca tcaccgtgag tacatccggc gtattcaccg gcatcaaacg ctttgtcgaa
     5761 gaaaaacaac cgtaccgttt ggcgctctcg ctgcacgcgg tgttccccga aaagcgtaac
     5821 cagctcatgg acatcgaaaa aaaattcccg tacgaagaag tgctcgatta tttgcgcgag
     5881 cacaagcaag atctgcaacc gggtcaactc atgttcgagt atattatgat taacgatatc
     5941 aatatttttc ccgaagacgc aaaagaactg acgcgttggg caaaactttt gggtgcgaaa
     6001 gtcaatctca ttccgatgaa caccacgttc aacggcatga cgcgtccgcc gcaagaaaaa
     6061 atcgatgcgt tttggaaaat gctagtgaag aacggcgtgg ttgcggtgaa ccgtaaatcg
     6121 ccagcgaaag acatcgacgg tgcgtgcggc atgctcgcgg gtagcctcat gccaagcgcg
     6181 ctttaagttt gagataaggc ccgccatcgg taactttcac ccattcttta taccctttgc
     6241 cacacatacc taggccgttg atatgcatat cgtgcgagac catcgaaatc gattgtaaaa
     6301 tttctggtac ataaccggtg acgatgaccg gtgaaaaata ctcgtcggtc aatttcccgt
     6361 ggcggatccg ccgtgcgact aacgcttcga gctgaattcc ccagcttttg ggatcttcca
     6421 tgccgttggt agcgcggtcg acaaagtaac cgtcctccac cgaggcaatc atcgtggcta
     6481 aatcgtcgtc ccccggcatg aagtaggtgt ttgtcatgcg cgaataaact ttgtgatcaa
     6541 agctctcgcg cctgccattg ggagagcggc ggaaatttaa ccgcgacgcg ctggtgtggt
     6601 cggtcatcgg ctgggtaagg attccttttt ctataatttt ggtttccgac gcaggcaatc
     6661 cctcgtggtc gaaatagtaa cttgccgcag cgccttctaa cgcaggcgag tcgtaaagcg
     6721 agacttgaga ggatgcgaca aatttaccta agtagtcgct accttttgcg cggccttttt
     6781 gcattgtgtc ggcttcggta ccgtggccaa acgcttc
//
LOCUS       DAOMPD010000075         6809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4792_length_6809_cov_2.554338, whole genome shotgun sequence.
ACCESSION   DAOMPD010000075 DAOMPD010000000
VERSION     DAOMPD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_6809_cov_2.554338"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLY93_03840"
     CDS             complement(<1..210)
                     /locus_tag="PLY93_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68639.1"
                     /translation="MCAALFLVFTVFLTCRSPEKLLDTDRSFAPLVDGGAFTLKALPP
                     IVQKVVVNFFAPHCPPCEKEVPELKK"
     gene            complement(283..1083)
                     /locus_tag="PLY93_03845"
     CDS             complement(283..1083)
                     /locus_tag="PLY93_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP68640.1"
                     /translation="MNDLKDKVVWVTGASSGIGRALVIEAAKQGAQVILSGRNQAALK
                     KVQSDAGLTATNSFVLPLDLQKYKALAKIVPRAVKAFGTIDVLFNNGGISQRSLTYET
                     KLQVYEDLMAVNYFGNIALSLAVLPLMRAKGKGIIATVSSVAGKFGTPLRSGYSASKF
                     ATVGFYEALRAENFDKNVQVTIVYPGFVNTNVAINALTATGKKQGVQDAATAAGITPE
                     ECAERAWKGILAGKNEVYIAGGKERFGMFVHKFFPSFFAKMIRKMKVT"
     gene            1201..4422
                     /locus_tag="PLY93_03850"
     CDS             1201..4422
                     /locus_tag="PLY93_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP68641.1"
                     /translation="MNLIRISILRPVFAWILMSALIIFGARVALRLGVSQLPDSDFPI
                     LSIKVTYEGATPDVIESSLLLPIEERLLGIEGLKEMKSTARQGLGQVVLELEMKRNVD
                     VALQEVQAALSQLRLPIGIDPPVITKTNPEDQPFMYISLFSDKPLHELIVWVDVFLLD
                     QFRFIPGIGEASITGYPERNIRVWPDMAKMRRYDLTVNDLVTAIETQHIESTAGQFVK
                     GKDELRVRWMGEASTPSDLAKVRIIRRGTENIYASGAALRIGDISRVEDGLSDQRRVA
                     RLNGSDLQGVSIGIRKQRGQNEVALADAVRQKVADLQTKLPEGYKLVVNTDFSRATKA
                     TVFTTYEKLIIAALVTILICFAFLGSWQTAVNILFSIPTSIVGTFLIVYFAGFTLNLF
                     TLLALTLAISIVVDDAIMLLENIVRHYRMGKNAAQAAFDGSMEILPAATAATLAVIAV
                     FLPVVVMDGIIGYFFFQFGIVMSAAVLLSLVEAVTITPMRTAAFLSVTPKAGKFELYL
                     EKLFEKFGDAYRRLLVKALKHPKMASGASFAMFALSMLLVFQIKQEFVPLQDQDYIII
                     SGQAAPGASLEYTLTRAKEYEQILKQNPNIALFSTSAGAGGPSAEINQFMIFVRLKPR
                     HERKAGHIQIMNDLRNQFKAIKGSWVSLRDFSSRGLTSGKQFQVSFNIFGSDLDILDA
                     KAKEIVERLNKEGLTQDLDTNFKKNFPELLISPNRDAMAARQVSIQSVSATLGATVAG
                     LREGRFTADGRRYDIRVKIPDEKITSPRDIGNILVRNTAGNVVPLSELVHFTENKTYQ
                     SINRVNRQRAIGIFGNVAPGKSQGVVLARAQKIAAEVLPQGYSFALEGGAAGLTDSFK
                     SLNLALFLGILIAYMILAVQFNSFVHPISVLVALPFSATGALITLWITGVSLNLFSFI
                     GLIVLMGIAKKNSILLVEFTNHVRNAQKQISVKEALIDACPVRLRPILMTSAATVIAA
                     LPLVFGSGVGHETRLPIGLTIVGGTIVSTVFTLFIVPCIYLLLTRFEKRHDVDLALKN
                     TPPHHFSSEKPPVELHSTNGYETISIADLEKPKKKLRSPKKAT"
     gene            complement(4419..4838)
                     /locus_tag="PLY93_03855"
     CDS             complement(4419..4838)
                     /locus_tag="PLY93_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68642.1"
                     /translation="MALTDSQKAELIRRGNTLFNAGRYFDAGRIFSTVGYKPGLIRLG
                     DLFYFEKEMPLVAYGFYRNAQHQPMLDKINDGFLFALQCLLGGEPSMASVRKNIPAKE
                     EQYKIRTANSSDSVGRTTTESVESKINRAVAMIKKLS"
     gene            complement(4880..>6809)
                     /locus_tag="PLY93_03860"
     CDS             complement(4880..>6809)
                     /locus_tag="PLY93_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68643.1"
                     /translation="RARLISEAELQENSMLKKFAVEDTKFAESLKAIAEKKNARLEEL
                     RKKGTPPFKDKDFMALANEYEKMVDKRDATLKSLLKYHEREKEHFAYAAQLIKEKDTA
                     IKASLKKIQEYQKLLAQIAAKKAPQDAPSLEETESALEIERQDLEAERAELAALKESA
                     SSIENNESKNKKEREKTNDLLIAEALLNLRDAALYGRIRLSLVSEQELLKLQRRNAKP
                     RQEQLDNFAAIRRFIYTAKNETDIPVARGRKSPLAGGVLAITRSEAEEQMHDLDSRPL
                     LGEKGLVHALLNENIAGYNMIVIDKTFGLERIWKSTQRLLIFSSVIGFLAVLAAWFFS
                     GMAVRRIQSLSKTSGEVKEGKLDVEFNDKGYDELSTLGQSLNAMVHGLKEREELRGEL
                     MAAEEIQKRLLPATVPLNLKARADIAGFYKAMVGIGGDYFDYVALGNDHIAIAMGDVS
                     SHGVGPALVMAMTRSQLHAELREKEVSLSKILLKLNEQLYLETPSHMFVTFFLGLYNL
                     KTGELQYISAGHSKPLFYSAASKKTTYLEAGGMPLGMDDNDFFESTMELRKVNLKHGD
                     IFLQYTDGLSEAMSATREQFGYERMEKALSEHTEKSPDGILQALAQSVEKFANTKLTE
                     PGPSSLSDDIALVCLKRA"
BASE COUNT         1670 a         1730 c         1520 g         1889 t
ORIGIN      
        1 cttttttaat tcaggcactt ctttttcaca cggcgggcaa tgcggcgcaa aaaaattcac
       61 caccactttc tgtactatcg gaggcaaggc tttcaacgtg aatgcgccgc cgtcgactaa
      121 cggcgcaaac gagcgatcgg tgtcgagtaa cttctctgga gaacggcatg taagaaaaac
      181 cgtaaagact aaaaaaagag ccgcgcacaa atacatccgc ctcaagagcc tgtgcgtcga
      241 ctcgctgaga aaattttgtg gagggaataa aaaatacatg cctcaggtaa ccttcatttt
      301 gcggatcatt ttcgcaaaga atgaagggaa aaatttatgc acgaacatcc caaagcgttc
      361 tttgccgccc gcgatataga cttcgttttt acccgctaaa attcctttcc atgcgcgctc
      421 tgcgcattcc tcgggggtga taccggcagc ggtggcggcg tcttgaacgc cttgtttttt
      481 gccggtagct gttagcgcgt tgatggcaac gttggtattc acaaaaccag gatacacaat
      541 tgtgacctga acatttttat caaaattttc agcacgtaat gcctcgtaga atcccactgt
      601 agcaaactta ctagccgagt agccagaacg caacggggtg ccaaatttcc ccgcaacact
      661 ggatacagtg gcgatgatcc ccttcccttt cgcacgcatg agcggcagga cggcgagtga
      721 aagcgcaatg ttgccgaaat aatttaccgc catcaagtct tcgtacactt gcaatttggt
      781 ttcgtaggtg agcgagcgtt ggctaatgcc gccgttatta aagagaacat cgatagtacc
      841 aaacgctttt accgctcgag ggacaatctt tgccagtgct ttatattttt gtaaatctaa
      901 cggtaggaca aatgagttgg ttgcggttaa accggcgtcg ctttgtactt tcttaagcgc
      961 cgcttggttg cgacctgaaa gaattacttg tgctccctgc ttcgccgcct cgataacaag
     1021 cgcacgacca atacccgagc tcgcccccgt cacccaaact actttatctt tcaaatcgtt
     1081 catgtagtcg ccttttatta gaaagaacgt tcggtcaacg taaattcatt tatgaagtga
     1141 aatcaccgat tgctttacgc aacgtttgca agacctaaaa ggacatattg gtatttattt
     1201 atgaatctta tccgcatttc tattctgcgc cccgtttttg catggattct catgtcggct
     1261 ctcattattt ttggcgccag ggtagcgctc cgcttgggtg taagccaact ccccgactcg
     1321 gattttccca ttttatctat caaggttacg tacgaaggag ccactcccga cgttattgaa
     1381 tcgagtttat tacttcccat tgaagagcgc cttttaggca tcgaaggctt gaaagaaatg
     1441 aagtccacag cgcggcaagg tttaggccag gtggttctcg aactcgaaat gaaaagaaat
     1501 gtcgacgtcg ctctgcaaga agtgcaagcc gcgctcagtc aattacgttt acccatcggt
     1561 atcgatcccc ccgtgatcac taaaaccaat cctgaagatc aaccgtttat gtatattagc
     1621 cttttttcgg ataaaccctt gcacgaactc atcgtctggg tcgatgtgtt tttactcgac
     1681 cagttccgtt ttatccccgg cattggcgag gcgtccatca ccggttatcc cgagcgtaac
     1741 atccgcgtat ggccggacat ggctaaaatg cgccgctacg atctcacggt aaacgatctt
     1801 gtaaccgcga ttgagacgca acacatcgaa agtaccgcag ggcaatttgt caaaggtaaa
     1861 gatgagttac gcgtgcgctg gatgggcgag gcgtccacgc cgagcgattt agcaaaggtg
     1921 cgcatcatcc gccgtggcac agagaacatt tatgcatcag gagccgcgtt acgtatcggc
     1981 gatatttccc gtgtcgaaga tgggttgagc gaccaacgcc gtgttgcgcg cctcaacggt
     2041 tcagatctac agggagtgag tatcggcatc cgtaaacagc gtggccaaaa cgaagtcgct
     2101 ctggcagatg cagtgcgcca aaaagtcgcc gacttacaaa ctaaattacc cgaagggtat
     2161 aaattggtcg tcaacaccga cttctcccgc gccaccaaag cgacggtttt taccacttac
     2221 gaaaaactta tcatcgctgc gctcgtcacc atcctcatct gctttgcctt tttaggaagt
     2281 tggcaaaccg ccgtcaacat tctcttttct atacccacat cgattgtagg tacatttctc
     2341 atcgtctatt ttgccggatt cacactgaac ctatttacat tactcgcgct tacgctcgcc
     2401 atctcgattg tagtcgacga tgcgattatg cttctcgaaa atattgtgcg tcattaccgc
     2461 atgggtaaaa atgcggcgca agcggcgttc gacggctcga tggaaatttt acccgctgcc
     2521 actgccgcaa ctctggcggt cattgctgtc tttttacccg tcgtggtaat ggatggtatc
     2581 attggctact tcttttttca atttggtatc gttatgtcgg cggcggtgct cttgtcgctc
     2641 gtcgaagcgg tcactatcac gccaatgcgt accgctgcat ttttaagcgt tactcccaag
     2701 gccggcaagt ttgaacttta tttagaaaaa ctctttgaaa aattcggcga tgcgtatcga
     2761 cgccttttag tgaaagcgct caaacatccc aaaatggcaa gcggtgcgtc ttttgcaatg
     2821 tttgcgcttt cgatgctgtt ggtctttcaa attaaacaag agtttgttcc cttgcaagac
     2881 caagactata tcatcatcag cggccaggct gcccccgggg catcgttgga atacacactc
     2941 acccgcgcta aagaatacga acaaatactt aaacaaaacc ctaatatcgc tcttttctca
     3001 accagcgcag gtgcgggtgg tccatcggct gaaatcaacc aattcatgat tttcgtacgt
     3061 ctcaagccgc gtcatgagcg caaagctggc catattcaaa ttatgaacga cttacgtaac
     3121 caattcaagg caattaaggg ttcttgggtt tcgctacgtg acttctccag ccgagggctt
     3181 acctcgggta aacaattcca ggtttcgttt aacatctttg gttccgattt agatatcctt
     3241 gacgccaaag caaaagaaat cgtcgaacga ctcaataaag aaggcttaac tcaagattta
     3301 gacaccaact tcaaaaagaa tttccccgaa cttttaattt cacccaaccg cgacgcgatg
     3361 gcggcacgac aagtctcgat ccaatcggtg tcggcgacgt taggggcgac agtcgccgga
     3421 ttacgcgaag gccgtttcac cgccgacggg cgtcgttacg atatccgcgt caaaataccc
     3481 gacgaaaaaa tcacttcccc gcgcgatatt ggtaacattt tagtgcgcaa tacggcgggc
     3541 aacgtcgtgc cgctttcgga attagtacac tttactgaaa ataaaacgta ccaatcgatc
     3601 aaccgcgtta accgacaacg cgccatcgga atttttggga acgtcgcgcc gggtaaatcg
     3661 caaggtgtcg ttttagcgcg tgcgcaaaaa atcgccgccg aggtgttgcc gcagggttat
     3721 tcgttcgcac tggaaggcgg tgcggcgggc ctcaccgatt cgttcaagag ccttaacctc
     3781 gcgctcttct tgggtattct cattgcgtat atgattttag cggtacaatt taattcgttt
     3841 gtgcatccca tttctgtgtt ggtcgcactc ccttttagcg caaccggcgc tctcatcacg
     3901 ctttggatta ccggcgtctc gctcaacctt tttagtttca tcggccttat tgtgctcatg
     3961 gggattgcta agaaaaattc aattttactc gtcgagttca ccaaccacgt ccgcaatgct
     4021 caaaaacaaa tttcagtaaa agaagctctc atcgacgcat gccctgtacg gctgcggccc
     4081 attcttatga cgagcgcagc gacggtgatc gccgcactac cgcttgtttt tggttcgggg
     4141 gtgggtcatg aaactcgtct ccccattggt cttactatcg ttggcggaac gattgtttca
     4201 acagtcttta cgctcttcat tgtgccgtgt atctaccttt tactcacccg ttttgaaaag
     4261 cgccacgacg tcgatttagc gcttaagaat acgccgccgc atcatttttc ttctgagaaa
     4321 ccaccggttg agttacacag taccaacggc tacgaaacaa tttccattgc ggatttagaa
     4381 aagcctaaga agaaattgcg ttcgccgaag aaggcgactt aacttaattt tttaatcatg
     4441 gcgactgcac ggttaatttt actttcgacg ctttctgtgg tggtacgtcc cacgctatcg
     4501 gatgaatttg ccgtgcgtat tttatattgc tcttctttag ccggtatatt ttttctaacc
     4561 gatgccattg agggttcacc ccccaaaaga cactgcaacg caaaaagaaa gccgtcgtta
     4621 attttgtcca acatcggttg atgctgtgcg ttacggtaga atccatacgc cactaggggc
     4681 atctcttttt caaaataaaa taaatcgccc aaacggatga gccctggttt ataccccacc
     4741 gtgctaaaaa tacgccccgc gtcgaagtaa cgccctgcgt taaaaagggt gttgcctcgg
     4801 cggatgagtt cggctttttg tgagtcggtg agagccattt acattttgta tcggcttttt
     4861 attgttttcc ctcgcatact tacgcccgtt tcaaacatac caacgcaatg tcgtctgaaa
     4921 gcgagctagg gccgggctcg gtgagttttg tattggcaaa cttttcaacg ctttgcgcca
     4981 acgcctgtag aatcccatcg ggggacttct cggtgtgttc cgacaacgcc ttttccatgc
     5041 gctcataacc gaattgttcg cgcgtcgcgc tcatcgcttc ggataacccg tcggtgtact
     5101 gcaagaaaat atcgccatgt ttcaagttga ctttgcgtaa ttccatggtg ctttcaaaaa
     5161 aatcgttatc atccataccc agtggcatac cacccgcttc aaggtatgtt gtcttctttg
     5221 aagcggcgct atagaagagc ggctttgaat gccccgccga gatatattgc aactctcctg
     5281 ttttgagatt atagagcccc aagaaaaacg tcacaaacat gtgcgacggc gtttcaagat
     5341 agagttgctc gttgagcttc aataaaattt ttgaaagcga aacttccttt tcgcgcaatt
     5401 cggcatggag ctgcgagcgt gtcatcgcca tgactaacgc cggaccaaca ccgtggctgg
     5461 agacatcgcc catcgcaatc gcgatgtggt catttcccaa cgccacgtag tcaaaataat
     5521 cgccaccgat tcccaccatg gctttataga atccggcgat atcggcgcgc gctttcaaat
     5581 taagaggcac ggttgccggt aaaagccgct tttgaatctc ttccgccgcc atgagttcgc
     5641 ctcgaagctc ttcgcgctct tttaatccgt gaaccatcgc gttgaggctc tgccccaacg
     5701 tacttaattc gtcgtagcct ttatcgttaa attccacatc gagcttgcct tctttaactt
     5761 cacccgaggt tttactcaac gattggatgc ggcgcacagc catgcctgaa aaaaaccacg
     5821 ccgccaaaac cgctaagaaa ccaatcactg aagaaaaaat caagaggcgt tgtgtggatt
     5881 tccatatccg ctctaaacca aaagttttat cgataacaat catattgtag ccggctatat
     5941 tttcgttgag caaagcgtgc acgagacctt tctcgcccaa aagaggacgg ctatctaaat
     6001 cgtgcatttg ttcttcggct tcagagcgag taatcgccaa gacaccacca gccagcggtg
     6061 attttcgtcc acgagcaacc ggaatgtcgg tctcattttt ggccgtataa ataaatcgac
     6121 ggattgcggc aaagttatcc aattgctctt gacgcggttt tgcattacgc cgttgcagtt
     6181 tgagcaattc ttgttctgaa actaaactca accgaatgcg accatagaga gcggcatcgc
     6241 gtaaatttaa aagagcctcg gcaataagta agtcattcgt cttctcacgc tcttttttgt
     6301 ttttactttc gttgttctcg atactcgacg cactctcttt gagggcggcg agttctgcac
     6361 gctcggcctc taagtcttgc cgttcgattt caagcgcgct ttctgtttcc tctaaagacg
     6421 gcgcatcttg cggggctttc ttcgctgcaa tttgcgctaa taatttctga tactcttgaa
     6481 tttttttgag cgacgccttt atggcagtat ctttttcttt tattaattgt gcggcataag
     6541 caaaatgttc tttttcgcgc tcatggtatt tgagcaacga ttttaatgta gcatcgcgct
     6601 tatcgaccat cttttcatac tcgtttgcca gagccatgaa atctttatct ttgaaaggag
     6661 gggttccttt tttacgcaat tcttctaaac gggcgttctt tttttctgca atcgctttaa
     6721 ggctttcggc aaatttagtg tcttcgacgg caaatttttt cagcattgag ttttcttgta
     6781 actctgcttc ggaaataaga cgcgcacgt
//
LOCUS       DAOMPD010000076         6763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4829_length_6763_cov_3.102266, whole genome shotgun sequence.
ACCESSION   DAOMPD010000076 DAOMPD010000000
VERSION     DAOMPD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4829_length_6763_cov_3.102266"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1015
                     /locus_tag="PLY93_03865"
     CDS             29..1015
                     /locus_tag="PLY93_03865"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRP68644.1"
                     /translation="MKTLIDRIGPSRVVLSPMAGFSDSPYRKLCRRMGSAYSITEFVS
                     TEQLFRSNPKAIQLFRYEEIERPIVFQIFGNDPDIILKGVERVLHLNPDGIDLNMGCS
                     VRTVSGSGSGAGLLKHPQKVREIIRRLVLETKLAISAKIRLGWDSASFNYKEITNILE
                     SEGVWSIAVHGRTRQMAYTGAADWDKIGEIAHARRVPIFGNGDVDSYPQAMEKIKTYG
                     VFGVFVGRGAVGNPWLFAGRKRTPLSYAERLPVILEHTQSMINFYGDSAAAVLMRKHL
                     TQYLRDTPEWNEIKRVAHHTTSAQELMEKLTSSSAETFSCVRQATKSLDISA"
     gene            complement(1226..2284)
                     /gene="fliN"
                     /locus_tag="PLY93_03870"
     CDS             complement(1226..2284)
                     /gene="fliN"
                     /locus_tag="PLY93_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRP68645.1"
                     /translation="MGDGSLSQEEIDALLMGTDDMAAPSAKGSGAQVADAGGLSPTER
                     ELLADAFNDAMQVAGQQAGVMAGKGIQISNAQVDEITPAQISNELPRGGVIAEIAMGN
                     NQTALVFPKDLARGIAQTMMGAPDDGGDINDAHLSTLSELSQSIISSIANGLGGKFNE
                     SLTPAVPDMKVFNSPADTPQFMGNIVKISYNMSVEGLSAARITHYIDAVAANRWAKSQ
                     RPSSPPQAAAFDMDFGAPQGNIGGGGVPVNPVNFPSLSQSSSTAGLPPNLELLLDVQM
                     ALTVELGRTKKYVKEILSLGEGSIIELDKLAGEPVDLLVNGKLIARGEVVVIDENFGV
                     RVTDIVGPAERMARMSGG"
     gene            complement(2318..2851)
                     /locus_tag="PLY93_03875"
     CDS             complement(2318..2851)
                     /locus_tag="PLY93_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRP68646.1"
                     /translation="MADAEDLDQDESTDGAAPPISSGRSRIVTILIWVVAGLIALALM
                     FVISVMVAKYYKNADFQETESITIAPPAAPLSVFSIKHEFRVNTADTDEAHYAQATIT
                     LGYEGELNKQLDAELGKRNPQIIHIINIILGGKRRDELFTPLQKLNLGEEIKNQINMI
                     LRDGKISEVMFEQLVVS"
     gene            complement(2868..3068)
                     /locus_tag="PLY93_03880"
     CDS             complement(2868..3068)
                     /locus_tag="PLY93_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRP68647.1"
                     /translation="MIELSRLNGKVFYLNHNLIEIFEETPDMVIKLTDGTKYVVREKT
                     DELLAKIIAFNRQIFAEKTRIE"
     gene            3164..3694
                     /locus_tag="PLY93_03885"
     CDS             3164..3694
                     /locus_tag="PLY93_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68648.1"
                     /translation="MNRIPPLSLFKSISFIAALVFIVIACSHAVIGKSTPSETSALPL
                     ITADGTCTGTNPTIKKCTVSFYIQPYKGAKILNDTETLPIKFSQIDKTMALATVWIND
                     VPSDEEKRALKVLQPSNDGVRGLDVLWRETPVVDFPLVALDAPTQKHRFVRRILTGKQ
                     PRVRCSFLKLKLYCRF"
     gene            3655..4563
                     /locus_tag="PLY93_03890"
     CDS             3655..4563
                     /locus_tag="PLY93_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRP68649.1"
                     /translation="MFISETQVVLPLLNDNHIEVVDIHTGSVTRLTLPPKYAKKGGFV
                     ESVRVPPLAEFWVSQMMASAVHRFDTATLQYKDSIDLKGKWTKVLAYDSARNTVYASN
                     WTSSDISVIPLTTLKEEHRIKMNGVPRGMAFSKDGEYMVAAIFGGKNDSDSSGRAVKI
                     DLAKEKILQTIIPGGAQRHVVRLKEGENRFAVSDMARSRVFFIDDYKKSDEIKVYNNP
                     NTIVESPDGKFLYVSCRGRNNPKSYLLKGFDFGRLLVIDLITRQIVESIEAGNQPTGL
                     DVSPSGRYVALTDFLDHAIRVYERVL"
     gene            4563..5345
                     /locus_tag="PLY93_03895"
     CDS             4563..5345
                     /locus_tag="PLY93_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68650.1"
                     /translation="MLQSLDFNRAFEDAAKGYAKNFAMAALILFVSFILILLSIFSFF
                     GMFFALPNLVAGISLVSYYMTKEKIGFSTIFSGFKSYWSVVGAMHIYWTGYLLLYLIF
                     SAPYYYHFISIFADTLNFSGSIDENEILKAFIAASTASSVQNAALFQYLAYPIQFYLQ
                     GRLLPLFPLIVDERLSIPAAFKKSWSMTRRVQWQLLLFIFLTMLIAFVSTLAGMLALF
                     VGIFFTLPFSLALVGAATKQLMEAANNYTQLPAAIPHPTAPL"
     gene            complement(5290..6399)
                     /locus_tag="PLY93_03900"
     CDS             complement(5290..6399)
                     /locus_tag="PLY93_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68651.1"
                     /translation="MRIIFSRYLIFIALVGCFSLYAEDAADKKTNAADAWQDVKPKTP
                     NTPSPKDDSASKEPAPQSEASAPAPTEQEKTVVLDEVADNTGKVFKAKVLGIGEHEIL
                     IRDETGAEKTISKENLIFTRLAVGRYYFFLPDKKETEKEAREASREKAGAWEIVVGVG
                     MHLADNSDVPAYLNEYAAHLSDYYKTTYATEFPVNKGIEGGALHWQFFVEHRFHYESH
                     FWALGAGYAMLPKSSSVVSSTVSEIQSTINVTGFFIPVYAAYYHRVWQSGALEFNLGF
                     GVGGMYTGIEVKTVGGPSANDQVLTSFTPFLMAKPEFTYQIGKLRILASMPIYWAQAH
                     NVTDGEETLMMYKSRNIVTTGLSGVGLQLAVGYSY"
     gene            complement(6435..>6763)
                     /locus_tag="PLY93_03905"
     CDS             complement(6435..>6763)
                     /locus_tag="PLY93_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68652.1"
                     /translation="ETDPVYTMVFDEVDAGVGGRIAEAVGKKLAGLSLASQVIVITHL
                     HQIAGLSPNEVRHFKVSKHKEDGTRITRLTKEQRLQEVARMIAGENINESALTHARTL
                     LAQKIT"
BASE COUNT         1641 a         1675 c         1578 g         1869 t
ORIGIN      
        1 atccaagttg cccgcgcgca gctctagcat gaaaacactc attgaccgca tcgggccttc
       61 gcgtgtggtg ctctcgccga tggccggttt ttccgattca ccgtaccgca aactgtgccg
      121 ccgtatgggc tcggcgtata gcatcaccga gtttgtttcc accgaacaac tcttccgttc
      181 aaacccgaaa gcaattcaac tttttcgtta tgaagagata gaacgcccca tcgtctttca
      241 aatttttggt aacgaccccg acattattct gaaaggagta gagcgcgttt tacacttaaa
      301 ccctgatggc atcgatttga atatggggtg ttctgtacgc acggtctcag gctcaggttc
      361 gggggctggt cttttgaagc atccacaaaa agtacgcgaa attatccgcc gcttagtttt
      421 agaaaccaaa ttggcaattt ctgcaaaaat tcgattaggc tgggatagcg cttcatttaa
      481 ttataaagag attaccaata ttttagaaag cgaaggtgtg tggtcgattg ccgtgcatgg
      541 gcgcacgcgc caaatggcgt ataccggcgc tgccgactgg gataaaattg gtgagattgc
      601 ccacgcgcgc cgcgtgccaa tttttggaaa cggtgacgtg gatagttatc cccaagcgat
      661 ggagaaaata aaaacgtacg gtgtttttgg cgtttttgtg ggacgaggtg ccgtgggtaa
      721 tccttggctt tttgctggcc gcaaacgtac accgctctcg tatgcagaac gattgccggt
      781 tatactcgaa cacacgcaaa gtatgataaa tttttatggc gatagcgcag cggcagtgtt
      841 gatgcgcaag cacctaacac aatacttgcg cgacacgccc gaatggaatg aaataaaacg
      901 tgtggcgcac catacaacct cggcgcagga gttgatggaa aaattaacgt catcttccgc
      961 cgaaaccttc tcttgcgttc gacaagctac aaaaagcctg gatatatccg catgattgtc
     1021 cagcgatttg gatagttatc ctcaaatgtg acataatgct ggatagttat cctcaaatgt
     1081 gacataatgc tggatagtta tccctaaatg tgacataata ctggatagtt attcctggta
     1141 atccaaagtt tgtatcacta agtctgtccg agtcctatcc tccggttctc gaagacgcga
     1201 acctacggat agccccgtcc gagttctatc ctccggacat tcgtgccatg cgttcagcag
     1261 gccctacaat gtcggtgacg cgcacgccga agttttcgtc gatgactaca acttcgccac
     1321 gggcaatcag ctttccattc acgagtaaat ccactggttc acctgcgagc ttgtcgagtt
     1381 caataatcga accttcgccc aacgataaaa tttctttgac gtactttttg gtgcgcccca
     1441 gttcgacggt gagcgccatt tgcacatcga gaagcaattc gaggtttgga ggtaatcccg
     1501 ctgtgctgga agattgcgag aggcttggaa agtttaccgg attcacgggc acgccaccac
     1561 cgcctatgtt gccttgcggt gcgccgaaat ccatatcgaa cgcggctgcc tgcggtgggc
     1621 ttgaagggcg ttggcttttt gcccaacgat tcgccgctac tgcatcgatg taatgagtga
     1681 tgcgtgccgc actcagccct tcgacagaca tgttgtatga aatttttaca atgttcccca
     1741 tgaactgcgg tgtatccgca ggtgagttaa acaccttcat gtcaggtaca gcaggcgtga
     1801 gcgattcgtt aaacttaccg cccaagccgt tcgcgatgga cgaaatgata ctttgcgaaa
     1861 gctcggagag cgttgaaaga tgcgcgtcgt taatgtcgcc accgtcgtcg ggtgcgccca
     1921 tcattgtttg tgcgataccg cgtgctaaat ctttggggaa gacgagtgcg gtctggttat
     1981 tgcccattgc aatctctgca ataacgccac cgcgcggtaa ttcatttgaa atttgcgccg
     2041 gtgtaatttc atcgacttgc gcgtttgaaa tttgaatacc tttgcccgcc atgacgcctg
     2101 cttgctggcc tgcaacttgc atcgcgtcgt taaacgcatc ggctaacaat tcgcgttcgg
     2161 tgggcgacaa accgcctgcg tcggcaactt gtgcgcccga gccttttgcg ctcggcgccg
     2221 ccatgtcgtc ggtgcccatc aagagcgcgt caatttcttc ttgcgataac gatccgtcac
     2281 ccatagtatc tcctaaacaa agaaggattt ccctctttta acttaccacc aactgttcaa
     2341 acataacttc tgaaatttta ccgtcacgca aaatcatgtt gatttgattt ttaatctctt
     2401 cacccaaatt gagtttttgt aacggcgtga aaagctcgtc tcgcctttta ccaccgagaa
     2461 tgatattgat aatatgaata atttgaggat ttctttttcc caattcggcg tccaactgtt
     2521 tattaagttc accttcatac cccaaagtga tcgtcgcttg cgcgtaatgc gcttcgtcgg
     2581 tgtcggcagt gttgacacga aattcatgct taattgaaaa caccgacaac ggtgcggcag
     2641 gtggtgcgat tgttatactt tcggtttctt gaaaatcggc gtttttataa tacttcgcga
     2701 ccatcaccga aataacaaac atgagcgcca atgcaataag gccggcaacc acccaaatta
     2761 aaatagtgac gatgcggctg cgtcccgaac taatcggggg tgcggcacca tcggtacttt
     2821 cgtcttggtc taagtcttct gcgtctgcca tacattccct ctatttatta ctctatacga
     2881 gtcttttcgg caaaaatttg gcgattgaat gcaataattt tggccaacaa ctcgtcggtc
     2941 ttttcgcgca ccacatattt tgtgccgtcg gtgagcttaa tcaccatatc gggagtttct
     3001 tcaaaaatct caatcaggtt gtggttgaga tagaagactt taccgtttaa gcgcgaaagt
     3061 tcaatcacta tgaattgcct ctattattat gtcggatgtt tcactcaagc cgttaagcga
     3121 atttcgtgtt ttcgacatgc gcagcctgtc cgatgtgtct ttgatgaatc gtataccacc
     3181 gctttcactt ttcaagtcga tctcttttat tgcggcgtta gtttttatag taattgcttg
     3241 ttcgcacgcc gtcatcggta agtctacacc atcggaaact tctgcgcttc ccctcatcac
     3301 tgccgatggc acctgtaccg gaaccaatcc cactattaaa aaatgcactg tttcatttta
     3361 tatccagccg tacaaaggtg caaaaattct aaacgacacc gaaacgctac ctataaaatt
     3421 ttcgcaaatc gataaaacca tggcgttagc gacggtgtgg atcaacgatg tgccaagtga
     3481 cgaagaaaaa agagcgttaa aagttttaca acccagcaac gacggtgtgc gcgggctcga
     3541 cgtgctgtgg cgcgaaacgc ctgtggtgga ttttccgctc gtggcgctcg atgcaccgac
     3601 acaaaaacat cgttttgtgc gccgtatcct cacaggcaaa caaccaagag tgcgttgttc
     3661 atttctgaaa ctcaagttgt actgccgctt ttgaacgaca accacattga agttgttgac
     3721 atacacactg gcagtgtgac gcgcctcacg ctaccgccaa agtacgcgaa gaagggcggt
     3781 tttgttgaaa gcgtgcgcgt gccgccgctc gccgaatttt gggtgagtca aatgatggcg
     3841 tcggcggtgc atcgtttcga cactgccaca ctgcagtata aagattcaat cgacctcaaa
     3901 ggcaaatgga caaaggtgtt ggcttatgat agcgcacgca atacggtgta cgccagcaac
     3961 tggacaagct cggatatctc agttattccg ctgacgactc ttaaagaaga gcaccgcatc
     4021 aaaatgaacg gtgtgccgcg cggcatggcg ttttctaaag acggcgaata catggtcgcc
     4081 gcaatttttg gcggtaaaaa cgatagcgat agttctggcc gtgcggtaaa aatcgacttg
     4141 gcgaaagaga aaattttaca aacgattatc cccggtggtg cgcagcggca tgtggtgcgt
     4201 ctcaaagaag gagaaaatcg tttcgccgtc tctgatatgg cgcgcagccg cgttttcttt
     4261 atcgacgact acaaaaaaag cgacgaaatc aaagtgtata ataaccccaa cacgattgta
     4321 gaatcccccg acggtaaatt tctttatgtg tcgtgccgag gccgcaacaa ccccaagtct
     4381 tatttactca agggctttga ttttggccgt cttttggtga ttgacttaat cacccgccaa
     4441 atcgtcgaaa gcatcgaagc gggcaatcaa cccaccggcc tcgacgtatc gcccagcggg
     4501 cggtacgtgg cgctcactga ttttcttgac catgccatcc gcgtttatga gagggtgctg
     4561 tgatgctaca aagtttagat ttcaaccgcg cgtttgaaga tgccgccaaa ggttatgcaa
     4621 aaaatttcgc catggctgca ctcatacttt ttgtaagctt tatcctcatt ctgctatcta
     4681 ttttttcttt ctttggtatg ttctttgctc tacccaatct cgtcgcaggg ataagccttg
     4741 tgagctatta catgacgaaa gaaaaaattg ggttcagtac tattttcagc ggcttcaaaa
     4801 gttactggag cgttgtcggt gcgatgcata tttattggac gggttatctt cttttgtatt
     4861 taattttttc ggcgccttat tattatcatt ttatatccat ctttgccgac acgttaaatt
     4921 tttctggctc aatcgatgaa aatgaaattc tcaaagcgtt tatcgcagca agcactgctt
     4981 cctcggtaca gaacgctgcg ctctttcaat accttgcata ccccatccaa ttttatttac
     5041 aaggcaggct cttgcctttg tttccgctca tcgtcgatga aagactttcc ataccagcgg
     5101 cgtttaagaa aagttggtcg atgacacgtc gggtgcaatg gcagctattg ctttttattt
     5161 tcctcacaat gctcatcgcg ttcgtctcaa cgttggcggg tatgttggcg ctttttgtag
     5221 gtatattttt tacgttacct ttttcgttag ccctcgtcgg cgcagcgacc aaacagctca
     5281 tggaagctgc taataactat acccaactgc cagctgcaat cccacacccg acagccccgt
     5341 tgtgacgatg ttgcgtgatt tatacatcat caacgtttct tcgccgtcgg tgacgttgtg
     5401 cgcttgtgcc caataaatcg gcatgcttgc taaaatgcgc agcttaccga tttgatacgt
     5461 gaactcaggc tttgccatga gaaacggagt aaaactggtg aggacttgat cgttcgcaga
     5521 cggaccacct acagttttca cttcgatgcc cgtgtacata ccgccgacgc caaatcccaa
     5581 attaaactct aatgcccccg actgccacac acggtggtag tacgcagcat acacgggtat
     5641 aaaaaaaccg gtgacgttaa tggtgctttg gatttctgaa acggtagagc tcaccaccga
     5701 cgagcttttg ggcagcatcg cataccccgc ccctaacgcc caaaaatgac tttcgtagtg
     5761 gaagcggtgc tcgacaaaaa actgccagtg taacgctccc ccctcgatac ctttgttgac
     5821 aggaaattca gttgcatatg ttgttttgta ataatcactg agatgcgcgg cgtattcatt
     5881 taagtatgca ggcacgtcgc tattgtcggc caagtgcatg cccacgccga cgacgatttc
     5941 ccatgcgccc gctttttcac ggctcgcttc acgcgcttct ttttcggtct cttttttatc
     6001 gggtaagaaa aaatagtacc gtcccaccgc aaggcgcgtg aaaattaaat tttcttttga
     6061 gattgttttt tccgcaccgg tttcatcgcg gattaaaatt tcatgctcgc cgatacccaa
     6121 tacctttgct ttgaaaactt tgccggtgtt atcggcaacc tcgtcgagta cgaccgtttt
     6181 ttcttgctct gtgggcgcag gcgcacttgc ttcgctttgg ggggcgggtt ctttcgatgc
     6241 cgaatcatct ttaggcgacg gagtatttgg agtcttaggt tttacatctt gccacgcgtc
     6301 ggcggcattt gtctttttat cggcggcgtc ttctgcatac agagaaaaac acccaaccag
     6361 cgcgataaaa attaaataac gactaaaaat aattctcatg aaagtataaa tccccctttg
     6421 aaataaaatg cgagctatgt aattttttgt gccaaaagag tgcgcgcgtg cgtcaacgcc
     6481 gactcgttga tattttcgcc cgcaatcatg cgcgccactt cttgcagtcg ttgttcttta
     6541 gtgagacggg tgatgcgtgt gccgtcttct ttgtgtttcg agactttgaa gtggcgcacc
     6601 tcgttgggcg aaagaccggc gatttggtgt aagtgtgtga tgacgatgac ttgcgaagcg
     6661 agcgaaaggc ccgccagttt tttaccgacc gcttcggcga tgcggccacc cacgcccgca
     6721 tccacttcgt cgaacaccat cgtgtacacg gggtcggttt caa
//
LOCUS       DAOMPD010000077         6739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4855_length_6739_cov_2.500449, whole genome shotgun sequence.
ACCESSION   DAOMPD010000077 DAOMPD010000000
VERSION     DAOMPD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_6739_cov_2.500449"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLY93_03910"
     CDS             <1..901
                     /locus_tag="PLY93_03910"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRP68653.1"
                     /translation="PIAHGNDGGGSIRIPAACCGLVGLKPSRGRTKDNAAAASIPINI
                     ISEGVLTRSVRDTAAFFYGIEQIYQNPKLPPIGRVEGPGKKRLRIGVVIDSITGTPTD
                     TETRATIEKTVQLLSAMGHRVETMPLPVASEFLEDFALYWGFLAVLITGFGRIAVSPD
                     FDTHKIEPLTRGLIDYYRKRILKTPGMFIRMAQADKTYTEAFQGWDAVLTPVTGHTVP
                     RLGHLSPSLSFEELWERLKAYVSFTPLNNAAGTPAISLPMGMTQEGLPIGVMLQTIPG
                     GERTLLELAYELEAAHPWVKITQ"
     gene            898..>1636
                     /locus_tag="PLY93_03915"
     CDS             898..>1636
                     /locus_tag="PLY93_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68654.1"
                     /translation="MKIPQRIDTFESHELAFVPDRLKVLFREILSWEQDMGGVYKAAS
                     SIVEEWLQKTRANAILDLCSGAGGPGVSLVRALRQKTQRAVQIKLTDLYPATNAYERL
                     ATQHGHFISYETHSFDAANSHRDAVFRVRTLLSAFHHFSPAFAQKILEDAATYSDGIC
                     IMDPFQRDLWHLLTVAVGTTLGTQPYPLLKEQSPFAFAMCNLTPVIPAMYFWDSVASV
                     LRGYTEDELIALTRTPRCAAFEWHTGTW"
     assembly_gap    1637..1669
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1709..2692
                     /locus_tag="PLY93_03920"
     CDS             1709..2692
                     /locus_tag="PLY93_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRP68655.1"
                     /translation="MNKGASISLLVSILLLVLKFVAYFLTNSKAVLSDALESIVNVSA
                     AALALWIIRVSQRPADSDHPYGHGKAEYFSSVFEGALISFAAIIIFFDSIEALVKGHT
                     TESIGVGLALIVGAGVVNALLGIYLIRLGKKVYSPALIASGKHVLSDFYTSIAVVVGL
                     TLVQLTGVQWLDPAAALVVGVFLVITGFKLIRRSIDALMDAKDTELIHKLGNYFTECR
                     TEGIIRIHHTRVVRAGNYHHIDAHVVVPEFWDVTTAHNRTNAFEKKVFHRYEHRGEIH
                     FHIDPCRRVYCHACDVKDCLIRRAPFERLIPFTFEEITSPIEPENLQLPEK"
     gene            complement(2699..>3161)
                     /locus_tag="PLY93_03925"
     CDS             complement(2699..>3161)
                     /locus_tag="PLY93_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68656.1"
                     /translation="WRRYFHGVPLSFRSQRQGDWGARLTSALEEALTKHDMVCVWPTH
                     IPTLPSGILETAATLYPKALFTPERHGGVAFFSLASIHATALKKITAVRWQTPAAAAD
                     FLKVLQAVGVPVVQRGRVSALRSVNDFPRVIRELEEKQDSAALQALYNTLE"
     assembly_gap    3162..3171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3172..3390)
                     /locus_tag="PLY93_03930"
     CDS             complement(<3172..3390)
                     /locus_tag="PLY93_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68657.1"
                     /translation="MRVAHVLFSDSAGEAALLPRTPPQWNLSRPTLSPVFVEWLWGNH
                     ARTLAAYRNKKHTTHAYFYCATPMPRWKA"
     gene            complement(3395..4189)
                     /locus_tag="PLY93_03935"
     CDS             complement(3395..4189)
                     /locus_tag="PLY93_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRP68658.1"
                     /translation="MQYLLIPIGFVFYIWFLAAYIPVTLLAALSVIILAPFWPKKYAH
                     KLRKIAETWGWIGVRITFCPVRIYGREKAHREPGIIISNHQSMFDIFVGLGFYPIDFL
                     FLSKEEIFKIPFVGLAMRKVGYIGVNRSSPRQAARSLDIVAERIRENNRVLIYPEGTR
                     SKNARKLLPFKAGAFQVAEKHGFPIFPIALYGTQEARPMHSNILLFPHRIAIQVLDPI
                     TKESDLHPANEKSPLSEKERMAGIRALVEKAYLQLADSKGKKPAVM"
     gene            complement(4230..5828)
                     /locus_tag="PLY93_03940"
     CDS             complement(4230..5828)
                     /locus_tag="PLY93_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68659.1"
                     /translation="MASSPLSSDASDTKEDISRRTFFSGIVGLLAILMGFFWFRWQRK
                     QVTARFVGPAVERGHLLRIEYKNNATGTSEKIDTAIIGGGVSGLSAAWYLNNHGYKNF
                     ALFELEDKVGGNASWGENRFSRYPWGAHYLPTPGKDATYVQELLREMGILKNGKYSEE
                     CLVHEVEERLFIYGIWQEGLKPLMGARAVDKKDFEAFRNLTQSYSKRKGSDGRPAFTI
                     PLALSSRDPQLLALDRITATEFLASQGFTSQRLFWYIDYSLRDEYGSNRSNTSAWALI
                     HYFAARGNMHNLVWPEGNGFLVEYMRSRVEPYIHTGKALRHIEKGTNDYALHFLDFAS
                     GQIRKVSAKNIIFSIPKFILSYLYPQLHAEKAAHTRAFTYSPWMTANFLVNHFDMENP
                     AWDNVIYGSHSLGYVVAEHQKIGKLPHARTLTFFHAFDEDDTLQSRRRLLSMSERGAL
                     DFALRELEIPHPHIHDSIEEVGVYRWAHAMIRPIPGFITGNIARLFDTIDRNFYGAHS
                     DLSGMSNFEEAQYRGVTAAKRILS"
     gene            complement(5843..6100)
                     /locus_tag="PLY93_03945"
     CDS             complement(5843..6100)
                     /locus_tag="PLY93_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68660.1"
                     /translation="MTDSILNKAPSADLWQGQSDEGELGFSYNAADHILYFYIEEKKP
                     LDELKKMAAELGEKESTVDTILSRIERFRFKRQLPPIVALT"
     gene            complement(6121..6630)
                     /gene="nadE"
                     /locus_tag="PLY93_03950"
     CDS             complement(6121..6630)
                     /gene="nadE"
                     /locus_tag="PLY93_03950"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRP68661.1"
                     /translation="MIRLPELDVEKTASTLVKNIRALFQEKEFERAILGLSGGVDSAL
                     ALALAREALGEKYIKGIIMPYKTSNPASQEDAIQLSQKFNTAYEVHPITPMADAYFER
                     DPQLDAARRGNIMARLRMLTLYDLSARDNALVVGTGNKTEILLGYSTLWGDTACGLNP
                     LGNLYKYQV"
BASE COUNT         1597 a         1677 c         1713 g         1709 t
ORIGIN      
        1 gccgattgca catggtaacg atgggggtgg ttcaattcgc atccccgccg cgtgttgtgg
       61 cctcgtgggg cttaagccat cgcgggggcg cacaaaagac aatgccgccg cagcatcgat
      121 tcccatcaac attatttctg aaggcgtgct cacgcgttcg gtgcgcgaca ccgccgcatt
      181 tttttacggt atcgaacaaa tttaccaaaa tccaaagctc ccgcccatcg gtcgtgtcga
      241 aggtccaggt aaaaaacgcc tgcgtatcgg cgtcgtcatc gattcgatca ctggaactcc
      301 caccgatact gaaacgcgcg ccactattga aaagacagtt caacttttgt cggcgatggg
      361 acaccgtgtg gagacaatgc cgttaccggt ggcgtccgaa tttttagaag actttgcgct
      421 ttattggggg ttcttagcgg tgctcatcac cggctttggt cggattgctg tttctcccga
      481 ttttgatacg cataaaatcg aaccgctgac gcgaggtctg atcgattatt atcgtaaacg
      541 tatcttaaaa acccctggta tgtttatccg gatggcacaa gctgataaaa cgtacaccga
      601 agcattccaa gggtgggatg cggtgttaac gccagttacc ggccacaccg tgccgcgctt
      661 gggacacttg agcccctcac tttcgtttga agaactttgg gagcgtctca aggcgtatgt
      721 gtcttttacc cccctcaaca atgctgcggg cactcctgca atttcgctac cgatggggat
      781 gactcaagaa gggcttccca ttggcgtgat gctgcaaacc atccctggcg gcgagcgtac
      841 cttgctcgaa ctcgcgtacg aacttgaagc cgcgcatccg tgggtaaaga ttacacaatg
      901 aaaatcccgc agcgcatcga cacattcgag agccatgagt tggcgtttgt gccagacagg
      961 cttaaagttc tctttcggga aatcctgtcg tgggagcagg acatgggcgg cgtgtataaa
     1021 gcggcttctt ccatcgtcga ggaatggttg cagaagacac gcgcgaacgc aattttagat
     1081 ctatgttctg gtgcaggtgg accaggcgtt tcactggtgc gggcgttgcg ccaaaaaaca
     1141 cagcgcgcag tacaaataaa acttaccgat ctttaccctg caacgaatgc gtacgagcga
     1201 ctcgcgacgc agcatgggca ctttatctct tatgagacgc acagcttcga cgccgcgaat
     1261 agccaccgcg acgccgtgtt tcgggtgcgt acgttgcttt cggcgttcca tcacttttca
     1321 ccggcattcg ctcaaaaaat tcttgaagac gccgccactt acagcgatgg tatctgcatc
     1381 atggatcctt ttcaacgtga tttgtggcat cttctcaccg tcgcagtcgg cacgacgctt
     1441 ggtacacaac cctatccgct cctcaaagaa caatcacctt ttgcttttgc gatgtgcaac
     1501 ctcaccccag ttatccctgc gatgtatttt tgggacagcg tcgccagtgt tttgcgcggc
     1561 tacaccgagg acgaactcat tgcactcaca cgtacaccgc gttgcgcagc gttcgaatgg
     1621 cataccggca cttgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gggctcaaag
     1681 gcaattattt aattgggttt cgtcgctcat gaataaaggc gcatcaattt cgctgctcgt
     1741 tagtatactc ttactcgtcc taaagtttgt tgcgtatttt ttaacaaatt caaaagcagt
     1801 tctctccgac gcgttagaaa gtatcgtcaa cgtgagtgcc gccgcgctcg cgttgtggat
     1861 aattcgcgtg agtcaaagac ccgccgacag cgatcatccg tatgggcacg gcaaagcaga
     1921 gtattttagt tcggtgtttg aaggcgcgct gatttctttt gcggccatta ttatcttttt
     1981 cgacagcatc gaagcgctcg tgaaaggcca caccaccgaa agtatcgggg taggtcttgc
     2041 gctcattgtg ggtgcgggtg ttgtgaatgc gctcttgggt atatatttaa tccggttggg
     2101 gaaaaaagtg tattcgcctg cgcttattgc tagcggcaag cacgtccttt ccgattttta
     2161 caccagcatt gcggtggttg tgggacttac cctggtgcag ttaaccggcg tgcagtggct
     2221 cgaccccgct gctgcgctcg tcgtgggggt atttctcgtc atcactggct ttaagctcat
     2281 acgcagatcg atcgatgcgc tcatggacgc caaagacacc gagctcatcc ataagttggg
     2341 aaactatttc accgagtgcc gcaccgaggg aattatccgc atccaccaca cccgcgtcgt
     2401 gcgggcgggg aattaccatc acatcgacgc gcatgttgtt gtgcccgaat tttgggatgt
     2461 caccaccgca cacaaccgca cgaatgcgtt tgaaaaaaaa gtgtttcacc gttacgaaca
     2521 ccgtggagaa atccattttc acatcgatcc ctgccgtcgc gtttattgtc atgcgtgcga
     2581 tgtgaaagac tgcctcatcc gccgcgcgcc gtttgaaaga ctcatcccgt ttacttttga
     2641 agagatcact tcgccgattg aaccggagaa cttgcagcta cccgagaagt aaaaacattc
     2701 attccagggt attgtacaac gcttggagcg ctgcgctatc ttgcttctct tccagctcac
     2761 ggatgacccg tgggaaatca tttacgctac gcagagcgct cacccggcca cgctggacaa
     2821 cgggcacacc gactgcttgc aacactttca aaaaatccgc tgcggcggcg ggggtttgcc
     2881 aacgcactgc cgtgattttt tttagcgcag tcgcatggat agatgctaaa gaaaaaaaag
     2941 caacgccgcc gtgacgctct ggggtgaata atgcttttgg atagagcgtt gcggcggtct
     3001 ctaaaattcc tgaggggagc gttggaatgt gggttggcca aacacacacc atgtcgtgct
     3061 ttgtgagcgc ctcctctaat gctgaggtca agcgcgcgcc ccagtcgcct tggcgctgag
     3121 agcggaatga caacggcaca ccatggaaat aacgccgcca gnnnnnnnnn ncgccttcca
     3181 gcgcggcatc ggtgtggcgc aataaaaata cgcgtgagtg gtgtgttttt tattgcggta
     3241 cgcggcaaga gtgcgtgcgt ggttacccca cagccattcc acaaaaactg gagaaagcgt
     3301 ggggcgggac aaattccact gagggggggt gcggggaagc aacgccgctt ctccggcgct
     3361 gtcggaaaaa agaacgtggg cgacgcgcaa aaccctacat caccgctggc tttttgcctt
     3421 tgctgtccgc gagctgcaaa tacgcttttt cgaccaatgc gcggataccc gccatccttt
     3481 ctttttcgct caacgggctt ttttcgtttg cggggtgaag gtcactctct ttagtaatag
     3541 ggtcaagaac ttgaattgca atccggtgtg ggaagagtag tatattcgag tgcatcggtc
     3601 gtgcctcttg agtgccgtaa agcgcgatgg ggaaaatagg aaagccgtgc ttctcagcga
     3661 cttggaatgc acccgctttg aatggcagaa gcttgcgtgc gtttttgctg cgtgtacctt
     3721 cagggtaaat gagtacgcgg ttattttcac ggattctttc ggcgacgatg tcgagcgaac
     3781 gcgccgcttg gcgtgggctt gatctgttga cgccgatgta accgactttg cgcattgcga
     3841 gtcccacgaa tggaatcttg aagatttctt ctttagagag aaaaagaaaa tcgatggggt
     3901 aaaaacctaa cccgacaaaa atgtcaaaca tcgattggtg gttgctgata ataatacctg
     3961 gttctcgatg tgctttttct ctgccgtaaa tccgcacagg acaaaaagta atacgcacgc
     4021 caatccaccc ccatgtttcg gcaattttac gcaacttgtg cgcgtatttt ttaggccaga
     4081 acggcgcgag gattatcacc gaaagtgcgg cgagtaacgt tacagggata taggccgcta
     4141 aaaaccagat ataaaagaca aagcctatag gaattaacag atattgcata agagcgtctt
     4201 cacctgtggt ataacacgtc aataaattct tatgatagga ttcttttagc ggcagtcacc
     4261 ccacggtatt gtgcttcctc gaaattgctc atacccgaaa ggtctgaatg cgcaccgtaa
     4321 aagttgcggt cgatagtgtc gaaaaggcgt gcgatgttgc cggtgataaa tccggggatg
     4381 gggcggatca tcgcgtgcgc ccaacggtag acccccactt cctcgataga atcgtggatg
     4441 tgggggtggg ggatttccaa ctctctcaat gcaaaatcca atgcaccgcg ctcactcatc
     4501 gataaaagcc gacggcggct ttgcaatgta tcgtcctcgt cgaacgcatg gaagaacgtc
     4561 agcgtgcgag catgcggcaa tttaccaatt ttttggtgtt ctgcgacgac ataccccaac
     4621 gagtgcgaac cgtatatcac gttgtcccac gcggggtttt ccatatcaaa gtgattcact
     4681 aaaaaattcg ctgtcatcca cggtgaatag gtgaatgccc gcgtgtgtgc tgccttttct
     4741 gcgtggagtt gtggatagag atacgacaaa ataaatttag gtattgaaaa aataatattt
     4801 tttgcgctga ctttgcgaat ctgccctgac gcgaaatcta agaaatgaag cgcgtaatca
     4861 ttcgtgcctt tttcaatatg ccgcaatgct ttacctgtgt ggatgtatgg ctctacacgc
     4921 gagcgcatgt attcgactaa aaagccgttg ccttccggcc acaccaaatt gtgcatattc
     4981 ccccgtgctg caaaataatg gatgagcgcc catgcactgg tgttcgagcg gtttgaacca
     5041 tactcgtcgc gcaacgaata gtcgatatac cagaaaaggc gttgcgacgt gaatccttgg
     5101 cttgctaaaa attcggtggc agtgatacgg tctaacgcga gaagttgagg gtcacgcgag
     5161 gagagcgcca gaggaatggt aaaggcgggg cgcccgtcgc tgccttttcg ttttgagtac
     5221 gactgtgtga ggtttctgaa tgcttcaaaa tcttttttgt cgacagcgcg cgcacccatg
     5281 agaggtttta atccttcttg ccagatgccg tagatgaaga ggcgttcttc aacctcgtgg
     5341 actaaacatt cttcgctata cttgccgttt ttgagaatgc ccatttcacg caatagctcc
     5401 tgcacatacg ttgcatcttt accgggggtt ggtaaataat gcgcacccca tgggtaacgg
     5461 ctaaagcggt tttctcccca cgaagcattc ccgccgactt tgtcttctaa ttcaaaaagg
     5521 gcaaagtttt tgtaaccgtg gttgttcaaa taccatgcag cggatagtcc actcacgcca
     5581 ccaccaataa ttgcagtgtc tattttttcc gaggtgccgg tcgcgttatt tttgtattca
     5641 atgcgcagaa ggtgaccgcg ctcgacggca ggccctacaa agcgtgcagt aacttgcttg
     5701 cgttgccaac gaaaccaaaa gaaacccatt aaaatggcga gcaagcccac aatgccggag
     5761 aaaaaagtgc gtcgactaat atcctcttta gtatccgaag cgtctgagga aagaggggag
     5821 gatgccattt gagcttatat gattaagtca atgcaacaat cggcggtaac tgacgcttaa
     5881 aacggaaccg ttcaatgcgg cttaaaatgg tatcgacggt gctttctttt tcacccaatt
     5941 cagcagccat ttttttgagt tcatcgagtg gctttttttc ttcgatgtaa aaataaagga
     6001 tgtgatcggc ggcgttgtaa ctaaacccca attccccttc atcgctttgt ccttgccata
     6061 aatcggcact gggagcttta tttaaaatac tatcggtcac tccaaatggc gcgcgagttt
     6121 ttacacttgg tatttatata aattgcccaa tgggttcaat ccgcacgcgg tatcgcccca
     6181 gagcgttgaa taccccaata aaatttcagt cttgttgccg gtgcccacca cgagcgcatt
     6241 atcgcgcgcc gaaaggtcat acagcgtcaa catgcgcagg cgcgccatga tattcccgcg
     6301 ccgcgcagcg tcgagttgag ggtcgcgttc aaagtacgca tcggccatcg gcgtgatggg
     6361 gtgtacttcg taagcggtgt tgaatttttg tgaaagttgg atggcatctt cttgcgaggc
     6421 gggattgctc gttttatatg gcataataat ccctttaata tatttttctc ccaacgcttc
     6481 gcgcgccaat gctagcgcca atgctgaatc gacccccccc gaaagaccta atatggcgcg
     6541 ttcaaactct ttttcttgaa agagtgcgcg tatattttta acgagggtgc tcgctgtttt
     6601 ttcgacatcg agttcaggta gacgtatcat gacgcattct gaaaatacgt ttcatcgtgt
     6661 cagtaagagt tacttcttaa ccacaatgtc taacccacgg acatacctga caaatgctac
     6721 gccgcaattg gggggtatt
//
LOCUS       DAOMPD010000078         6731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4865_length_6731_cov_3.471090, whole genome shotgun sequence.
ACCESSION   DAOMPD010000078 DAOMPD010000000
VERSION     DAOMPD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_6731_cov_3.471090"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1723
                     /locus_tag="PLY93_03955"
     CDS             314..1723
                     /locus_tag="PLY93_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRP68662.1"
                     /translation="MACKAGINDSSVVNSVYTFGNDPVVIYTSPTDNATGVLPCDGTP
                     CRAKIILQFDRSMNTGLLAPTLTTGIYNGSSYINAPVNTTHIWKTTRYSNDTLTLHLS
                     WVWFPENSKIQWSLDKTNLKDTGDNGLTTNVQQTFTTTGAKQSFSLADTGQTTCYNDS
                     GAQACGNVSWPGQDADYADTPNARSFTGPTAHTTYTSDYTTKDNITSLVWKSCTEGIS
                     GSTCSTGSAPQMTWYNALNQCATLNTSNSGVGYAGRTDWRLPTMRELEMLPNLGVYNP
                     AIDTARFPATVSGTYWSSSTYPSAIISAWGVGFSAGNSGSAGKTTLYSVRCVSSSSAG
                     SVQALTDNDDGTVTDTTTNLVWQKCSYGQDNDAVCSNSAGTRTWQQALQYCNALALNG
                     RAWRLPSVVELLSIADKTKSSPALDSTSFPATVSGTYWSSTPYTSDVNKAWIVYSGTG
                     FSASTAKMNNNPVRCVADE"
     gene            2073..2690
                     /locus_tag="PLY93_03960"
     CDS             2073..2690
                     /locus_tag="PLY93_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68663.1"
                     /translation="MNPYIIAIEFCSRVLIENIFQEENRIEQLRKDLIQAEQRARNSE
                     FDFNNESMLEDPNELKLQKSYIAAMRGRENVSNLSNQVKELELTILAKQVSINSLAMA
                     LLQTAKQGISIVHQNLQNCPNGRLVKKSEYLKNIIWQGRNQAMHYEEGNYNQDVIQCF
                     NNLSLVDPTLDLQRYGNKSKAKEIIDLLDWRTYEAYEQDMNSILK"
     gene            2820..3461
                     /locus_tag="PLY93_03965"
     CDS             2820..3461
                     /locus_tag="PLY93_03965"
                     /inference="COORDINATES: protein motif:HMM:NF021106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRP68664.1"
                     /translation="MLRSSDRNSPFRRSLHHLINLANTDELVLCSGYFWEPGNSGYSL
                     LSDELYNQLSVARSNNLLSQGLTLIGGMFKPEGSVDWLDHFKNFGNKIQNAGIRVSAF
                     EMNQKNWHAKVAIRLAKGVPVAAIVGSSNLTGPAYREQFYRWNYEADIYIWIKSINAS
                     VENLPNANPKWPDILAEMNHKNIWNEEEHLKRIYKEIIENKGLHKVDISKPRK"
     gene            3721..4389
                     /locus_tag="PLY93_03970"
     CDS             3721..4389
                     /locus_tag="PLY93_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68665.1"
                     /translation="MAKQVIILPFKNKFLSDIDKCSRIYNKCAKLTAVRNSEKIHPKQ
                     LARITEMTFIDLVSAWEIFLENIFLRYLLGAKCPNDSDPNRFITHIKKLKEARGLLSG
                     LENYDAEKQYMRFIDTKWVIDRSKYFFKDGKPFADLQPHINKFVAATKIRNAIAHNSP
                     KSKKDFLDTAKNTFNIKNQGLHPGELLNKYATVQFNLGRATKKTYYEAYSELYKSMAE
                     VLAP"
     gene            complement(4638..4853)
                     /locus_tag="PLY93_03975"
     CDS             complement(4638..4853)
                     /locus_tag="PLY93_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68666.1"
                     /translation="MPKTLEADVTDMVKPYYERGLERGIEKGLKRGIEKTKLETARRM
                     FAKGLSLHDILEITGLSPADLRAAGLV"
     gene            complement(4939..5478)
                     /locus_tag="PLY93_03980"
     CDS             complement(4939..5478)
                     /locus_tag="PLY93_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68667.1"
                     /translation="MAKGFFRLFGYSVKTEFEIFKLPQKVDVVILRSKRTTQPASNGF
                     TVLDYFLEHNLISFKLFKDNFSTADVLELIIYFVGYINAEKSARIDNTTATLIVTKMP
                     TKVNQMTGLHKLAEGKYILKMNLFSVYIVNVNEIEITGNDAYFLIGYGKSERLQSFIP
                     KIKEKATTPRERKIVDKIN"
     gene            5727..5957
                     /locus_tag="PLY93_03985"
     CDS             5727..5957
                     /locus_tag="PLY93_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRP68668.1"
                     /translation="MVQVLSRYIDRRPNVCDGRPMIKGTRIPVASLVTHYRAGMSLDD
                     ILAGYPNITPAQLFDALSYYFDNKDEIDQDIE"
     gene            complement(6294..>6731)
                     /locus_tag="PLY93_03990"
     CDS             complement(6294..>6731)
                     /locus_tag="PLY93_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP68669.1"
                     /translation="TGIVVLAVPFSLLGALLLLWAMGIQISIAVWVGFIALMGLDAET
                     GVFMLMYLDLEYYARQKKIHNERDLIEAVYAGAVHRLRPKMMTVLAGFCGLLPIMFSS
                     ATGSDVMKAIAAPLVGGLFTSFILELVIYPPVYFLWRRRALVV"
BASE COUNT         2054 a         1388 c         1471 g         1818 t
ORIGIN      
        1 cacgattagc tcaagcgcga cgtggcaatg gttggcgcgt gatgcgaagg ggaatgtgag
       61 tggcgtggag agtgtggggt ttgtggtgga cgcaaacgca agtgagacta cgcaaaaccc
      121 cattcttaac ccatcggctg gtgtatatgc tagcactcaa agcgtatcaa tcacctcaag
      181 cacaccagat gctgtgattt gctatacaaa tgacggtgta acgccaagtt gtaatgcagg
      241 taaaaccgcc tgtgcgactg gtactcttta cagtggcgca ttcaatattt caagttcggt
      301 tttaataaaa actatggctt gtaaggccgg tataaacgat tcaagtgttg taaacagtgt
      361 ttataccttt ggcaacgacc ctgtggttat ttatacatcg ccaactgaca atgccactgg
      421 tgttttgccg tgtgacggca caccttgtcg tgctaaaata atattgcaat ttgatagaag
      481 tatgaacacc ggattacttg caccgacgct cacaacgggg atttataatg gctcatcgta
      541 tatcaacgcg ccagtaaata ctactcacat atggaaaacg acacggtata gcaatgatac
      601 tttaacgctg cacctcagtt gggtttggtt ccccgaaaac tctaagattc agtggagtct
      661 tgataaaaca aacctcaaag acacgggtga caacggtcta acgacgaatg tgcagcagac
      721 gtttaccact actggcgcca aacagagttt tagtttagca gataccggcc aaactacctg
      781 ctataatgat agtggtgccc aagcgtgcgg caatgttagt tggccaggcc aagatgccga
      841 ctatgccgac acacctaatg cacgcagttt taccggcccc acagcgcaca cgacgtacac
      901 gagcgactat acaactaaag ataatattac aagtttagtg tggaagtcgt gtaccgaagg
      961 catatcgggt tccacttgca gtacgggtag tgcacctcaa atgacctggt acaatgcgct
     1021 caaccagtgt gcaacactca acaccagcaa ctcgggggta ggctatgcag gtcgtaccga
     1081 ctggcggtta ccgacgatgc gtgagttaga gatgttgcca aatttgggtg tgtataaccc
     1141 agcgattgat acggcgcgtt ttccagcgac agtttctggc acttattggt cgtcgagtac
     1201 ctacccatct gctatcatta gcgcgtgggg agtcggcttc agtgctggca actcgggtag
     1261 tgccggtaag acaactctct attctgtgcg ttgtgtgtcg tcctcttcgg caggctcagt
     1321 gcaggctctc accgataacg acgacggtac ggttaccgat acaacgacca atttagtttg
     1381 gcaaaaatgt agttatggac aagataatga cgcagtgtgc agtaactcgg caggcacacg
     1441 cacttggcag caagcactac aatactgtaa tgcactggca ttaaacggta gagcttggcg
     1501 actaccttct gtggtagaac ttttgagtat tgcagataaa acgaagagta gcccagctct
     1561 cgatagcact tcttttccag cgacagtttc tggcacttat tggtcgtcga ctccttacac
     1621 atctgatgtc aataaagcgt ggatcgtcta ctcaggtact ggcttctcag ccagcaccgc
     1681 taagatgaat aacaacccag tacggtgcgt tgcagatgag tagccccacc ccgacctacg
     1741 gtcttcccct cccctgcaca cttcgacagg ctcagtgcag gcgcggagag gggctttcgg
     1801 gcatttgcct ttggcaaatg ggggtgagtg tatagatgcg cgttcggcgc gtctgggtgg
     1861 ttatctttct taggcagccg cgggctgcct aagaaagcgg ttgaggtccg gtgcgtggtg
     1921 tgatagtttg tgtgaagaat agattttgga agaggaaaag attatgaaaa atgagaagat
     1981 aaagaaagcg gtatggttag attttataaa atacgataat attaaacgaa gcgagcagca
     2041 agtaattttt tatttatttg catgaggcat atatgaatcc atatattatt gctatagaat
     2101 tttgctcaag ggtattaata gaaaacattt ttcaagaaga aaataggata gagcagttaa
     2161 gaaaagacct aattcaagct gagcagagag ctcggaattc agaatttgat ttcaataatg
     2221 aaagtatgct tgaagatcct aatgaactga aattacaaaa atcctacatc gctgcaatgc
     2281 gaggacgaga aaatgtatca aatttaagta atcaagttaa agaattagag cttacaattt
     2341 tagcaaagca agtttcaata aattcacttg caatggcttt gcttcagacg gcaaaacaag
     2401 gaatctctat tgtacatcaa aacttgcaaa actgtcctaa tggcagactt gttaaaaaat
     2461 cagaatactt aaaaaatata atatggcagg ggcgaaacca agcaatgcac tatgaagaag
     2521 gcaattataa tcaagatgta atacaatgtt tcaataacct gagtcttgta gaccctactc
     2581 ttgacttgca aagatatgga aacaaatcaa aagctaaaga aattattgat ttactagact
     2641 ggcggacata cgaggcttac gaacaagaca tgaatagcat tttgaaataa aattgtgcac
     2701 atgagttcag aaatttatag tcagattttg taccttgcac tactcgatac taaagcgtct
     2761 tatgaatata gccttgttcg ctattatcta agaaggaggt ctttttggga cttaggataa
     2821 tgctacgaag ttcagaccgg aacagcccat tcagacgtag tctacaccat cttattaact
     2881 tagcgaatac ggatgaatta gtattatgtt caggatattt ttgggaacct ggtaacagcg
     2941 gatactccct gctttctgat gaactttata atcagctttc tgtcgctcgt tcaaataact
     3001 tgctatctca gggacttacc ttgataggtg gaatgttcaa acctgaaggt agcgttgatt
     3061 ggttagatca ttttaaaaat tttgggaata aaatccaaaa tgccggaatt cgagtttcag
     3121 cctttgagat gaatcaaaaa aattggcatg caaaagtcgc gattcgttta gctaaaggtg
     3181 tacctgtagc ggcaatagtg ggctccagca atttaactgg gccagcatac agagaacaat
     3241 tctacaggtg gaattatgaa gctgacattt atatatggat taaatctata aatgcgagtg
     3301 tggaaaatct tccgaacgca aatccaaagt ggcccgacat tctagcagaa atgaaccata
     3361 agaacatttg gaatgaagaa gagcatctaa agcgaatata taaggaaatc attgagaata
     3421 aaggattaca taaagttgat atttctaaac caagaaaata gttttcactg ggaaataatg
     3481 gctgaggata agtattatgt gttgcgtcgc aactatttca tttaatcgcg tatctgttct
     3541 acgtgtcgga gatactccca tcgtataaac gggagatttg tttagtgttt ccataggagg
     3601 cgttacgggc atagttgatg atacaaagct gttttacaga ttttaaaata atgacgatgg
     3661 catcattaaa tagcagatta attcaacttt gtgagaagtt ttcggcggta gtttttactc
     3721 atggctaagc aagtaattat tcttccattt aaaaataaat ttttgtccga tattgataag
     3781 tgttcaagaa tctacaataa atgcgctaaa ctaacagcgg ttagaaattc tgaaaaaatt
     3841 catccaaagc aactggcaag aatcaccgaa atgactttta ttgatctggt ttccgcatgg
     3901 gaaatattct tagaaaatat tttcttgagg tacttgctcg gtgcaaaatg tcctaatgac
     3961 tcggatccaa atcgcttcat tacgcatata aagaagttaa aggaagctcg agggctccta
     4021 tctggtcttg aaaactatga cgcggagaag cagtacatga ggttcattga caccaaatgg
     4081 gttattgata gatcaaaata tttctttaaa gatggaaaac catttgctga tttgcagcct
     4141 cacataaata aatttgttgc cgcaacaaaa ataagaaacg ccatagccca taattcaccc
     4201 aagagcaaga aagattttct tgatacagca aagaatacct ttaatattaa gaatcaaggc
     4261 cttcatccag gcgaattact gaataaatat gctactgtac aatttaactt aggtagagca
     4321 acaaagaaaa cttattatga agcatatagc gaattataca aaagcatggc tgaagtgttg
     4381 gccccatgat gaagcacaag cggcgcccaa cgagactata gaaaaagcca ctaacacttc
     4441 acgggcgcat gttatttatt agaaaatagc aaaacaattt tcataaaaaa gaattttttc
     4501 tacagccctg tgcttcgata caaatttacc tacggcaaat tgattcggga cagcccccct
     4561 ctctctacaa ataggagcct gtcccgagtg attcgctgta aagcgaatcg taccgaggga
     4621 caggacacag gacagggcta taccaacccc gccgcgcgca agtcggcggg cgagagaccg
     4681 gtaatttcga gtatgtcgtg gagcgataat cccttggcaa acatacggcg ggctgtttcg
     4741 agtttggttt tttcaatccc tctcttcaac cctttttcga tacctctttc aagccctctt
     4801 tcataatacg gcttcaccat atcggttacg tcggcttcta aagtctttgg catatactcc
     4861 ccccgatgat tgacaagttg aaatatttag tatttttggg gcgtagcccc aaaaatacta
     4921 aattattaac ttatcaaatt aatttatctt gtctacaatt tttcgctctc tcggtgtcgt
     4981 ggctttttct ttgattttag gtataaaaga ttgcaaccgt tcggattttc catagccgat
     5041 aagaaaatac gcgtcgttgc cggtgatttc aatttcgttc acgttgacga tgtaaactga
     5101 aaagagattc atctttagga tgtacttacc ttctgccagt ttatgcaacc cagtcatttg
     5161 gttaactttc gtgggcattt tggtgacaat gagcgtagcg gttgtgttat cgatgcgtgc
     5221 cgatttttct gcattgatat aacctacgaa ataaatgatg agctcaagaa catccgccgt
     5281 agaaaaattg tctttgaaca atttgaagct gatgaggtta tgctccagaa aataatcgag
     5341 tacagtgaag ccgttgctcg ctggctgagt cgttctcttg ctgcgtaaaa tcacaacgtc
     5401 tactttttgg ggtagcttga aaatctcaaa ttcggttttg accgaatagc caaagagtct
     5461 aaaaaatccc ttcgccatat atttgaaaaa ttcgtcaaat ttgagtatga atcgcactgg
     5521 tgcctctata tattaaatac cagaaaacac cgtttttttc tcacttttga gaaatttttt
     5581 tgaaaaaagt gtgatttttt tgttctttct ctctttgcgt ggggctctct aaaccaaccc
     5641 cgccgcgctc aagtcgcctg cagggggtgg aatatcctat ttacaaaaag agcttgtaaa
     5701 actactaaag catagttaga tagtctatgg ttcaagttct cagtcgatat atagatagaa
     5761 ggccaaatgt ctgcgatggt agaccgatga ttaaaggcac tagaatacca gtggcttcac
     5821 ttgtaacaca ttatcgagcc ggcatgtctt tagatgatat tcttgcaggg tatccgaata
     5881 tcacacccgc tcaattattt gacgctctta gttattactt tgataataaa gatgaaattg
     5941 accaagacat tgaatgattc aatttttatt tactgcgaag cgtgaaacga ctgagaacca
     6001 aatcatttat ttataattta tttgtataag tgctggaagg tcggcatata ttcagacgca
     6061 cggttattga gcccatgcca ccgaagacgg agacccgaaa cgggtcgcga tacaaatttg
     6121 cctacggcaa attcactcgg cacaggctcc cactctactg agaaaaacat cacgactcta
     6181 tttacaaaga gtagaatctt gcaccgctct ctacaatagg agcctgtccc gagtaaaact
     6241 cccgaaggga gttttgtacc gagggacagg agcagacaca ggacagcctc tccctacacc
     6301 accaacgctc tcctccgcca cagaaaatac actggcggat aaataacaag ctctaaaata
     6361 aaactggtga aaaggccgcc cacaagcggt gcggcgatag ccttcatcac gtcagagccg
     6421 gtggccgacg agaacataat cggcaagaga ccacaaaagc cggcgagcac cgtcatcatt
     6481 ttggggcgca ggcgatgcac cgcacccgcg tacaccgctt cgattaaatc gcgttcgttg
     6541 tggatttttt tctgccgcgc atagtattcg aggtcgaggt acatcagcat aaagacaccc
     6601 gtctcggcat cgagccccat gagcgcaatg aatcccaccc acaccgcaat cgaaatttgg
     6661 atgcccatcg cccacagtaa gagcaacgca cccaagagcg aaaacggtac cgctaaaacg
     6721 acaatgccgg t
//
LOCUS       DAOMPD010000079         6682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4911_length_6682_cov_3.468840, whole genome shotgun sequence.
ACCESSION   DAOMPD010000079 DAOMPD010000000
VERSION     DAOMPD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_6682_cov_3.468840"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="trpB"
                     /locus_tag="PLY93_03995"
     CDS             complement(<1..898)
                     /gene="trpB"
                     /locus_tag="PLY93_03995"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRP68670.1"
                     /translation="MASKKTAAKAKNGKTNSSQKIISPPEGYFGNFGGRYVSDLLIPA
                     LDELNTAWKKFSKDAKFQKELLHLYNDYAGRPSLLTYAPNLSAAWQARVYLKREDLLH
                     TGSHKINNTLGQTLLAQKMKKGRIIAETGAGQHGVATATAAALMKIPAHIYMGTEDLR
                     RQSLNDFRMRLLGATVSGVGGEKGTLRDAVNEALRDWAKNVDDTYYLIGSAIGPHPYP
                     TMVRTFQSVIGREARQQFTKATGKLPQAVFACVGGGSNAIGMFSAFLKDSKVELIGAE
                     AGGRSLTPGNHSASLTQGKPGVL"
     gene            1224..1655
                     /locus_tag="PLY93_04000"
     CDS             1224..1655
                     /locus_tag="PLY93_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP68671.1"
                     /translation="MKKILFLLAAISLVACNKPEGNKANAKQPEQGQSKGIGPVQSVT
                     LAGLDAGLAQKGEKLFHEQCSACHKIDERYVGPALKGVTTRRTPEWVMNMIVNPEQMT
                     REDATAKELLGEYMAQMTNQNVVEADARMILEYFRKVDGVK"
     gene            1795..3669
                     /gene="nosZ"
                     /locus_tag="PLY93_04005"
     CDS             1795..3669
                     /gene="nosZ"
                     /locus_tag="PLY93_04005"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRP68672.1"
                     /translation="MAIFALSCRGKGAKDLLAKNAAERVYVAPGDKDEVYLFTSGGFN
                     GQIGVQGIPSGRLIKTIPVFSVWTENGYGFSDETKQMLNTSHGYLPWDDSHHIEASQT
                     KGKQDGRWIFINGNNTPRIARISLESFETEEIIEIPNSAGNHASPFLTENTEYAMAAT
                     RFSVPIPQRDIPIESYGKGEFHGTITMVGIDAKTGKLSLKGQVLVPGYDYDLSHCGKG
                     PSHDWCFFTTYNTEQAHKMLEIEASKNDKDFILAFNWKKAEACFNEGKGKTVSANYVH
                     NMRGHNSKLSTSKKFTSIKLMNPKDCDNIMYYMPTPKSPHGVDIDPTGEYIVGGGKLA
                     TVIPVHSFSKLTKAIQDKANFSETRMGIPVLKYEATLAGEVQKPCLGPLHTEFDGKGY
                     AYTSCFVTSEIVKWELGTWEVKQNMPAYYAIGHLSIVGGDSAKPYGKYLVALNKITKD
                     RYLPTGPELVQSAQLWDISGSKAELLLDFPTQGEPHYAQMIPADLIKDKTQKIFELSK
                     NEHPYAAKSEKDAKVVREGNVVHVYMTAIRSHFKPDTVELKVGDTAYFHVTNLEQDYD
                     IPHGFAIYGAPLPNILIMPGQTLSVKYEAKQLGIFPFYCTDFCSALHQEMQQYVRVSK
                     "
     gene            3733..4335
                     /locus_tag="PLY93_04010"
     CDS             3733..4335
                     /locus_tag="PLY93_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68673.1"
                     /translation="MKFLKTTIQPLHKKMLIAIAALMGIVYFIPIWHISLVAPQYPEG
                     LSMNIFVHSIKGGNAHDLTNINLLNHYVGMHEIIEESIPELGFMPYVLAFMILGAVVT
                     FFHARIYMLILGVLNLFLVASAGLFDFWRWEYNYGHNLNPNAPIIVPGMTYQPPLLGC
                     KTLLNIEACSWPHIGAILLFISVAILLVILYREKKLNSGA"
     gene            4335..4787
                     /locus_tag="PLY93_04015"
     CDS             4335..4787
                     /locus_tag="PLY93_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRP68674.1"
                     /translation="MSIIRFSIILVLLTVAQCNVPEPVEIKVGSQQCAHCRMGIVDMR
                     FNAQLVTTKSKRYHFDSIECMEEWKAEHSAIKIRRAFVKNTAAVNLYIDYLDARFVQS
                     ERLPSPMGAYLAAYATEDEVKEAIRAFGGREIQRSALLTASKNAQTQQ"
     gene            4844..5185
                     /locus_tag="PLY93_04020"
     CDS             4844..5185
                     /locus_tag="PLY93_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68675.1"
                     /translation="MGFLSLLTPCLEAKTITVCSTCQYISPKTAIDGAQHGDTIIFDG
                     GIYNVGTLTVSKRLSLVGKKNPIFDGENKGHILDLRADNITIEGITLKNTGVSDLKEF
                     AAIHAESLNHC"
     gene            5198..6070
                     /gene="nosD"
                     /locus_tag="PLY93_04025"
     CDS             5198..6070
                     /gene="nosD"
                     /locus_tag="PLY93_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRP68676.1"
                     /translation="MFYNATYGIYLARSHNCFIEENISHGNAQNEILGGNGIHLWYSD
                     DNTIRQNVLKGHRDGIYFEFSQNLHVEKNVATENIRYGLHFMFCHNSIITENRYFMNA
                     TGVALMYSKNLNLTANEFSDSRGPSMQGILLKDITDSTVQKNKIAHNTTGLLADNSSR
                     NKIQQNTFVMNGRALEVFGNAQDNRFENNSFLQNIFDVAVNSKENTNTYEKNYWDKYK
                     GYDLNHDGIGDVSFHPVQIFSFWVSKYPELSILAGSSMIYFLEIIERAFPVLTPTSLR
                     DMRPLMQKPIYTRM"
     gene            6070..>6682
                     /locus_tag="PLY93_04030"
     CDS             6070..>6682
                     /locus_tag="PLY93_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP68677.1"
                     /translation="MANFLTLENIRKTYLKRRRVLEDISLTLPQGQIVALVGPNGSGK
                     TTLMKILMGLTHADVGSSLYLDGEKTLFDLPSRISVGYMPQNPLFPDNLRVREIIEYI
                     RGFSQNIPLYYDALFTELNVASFHEKRFRELSGGMRQKLSVLQAFMYERALLVLDEPS
                     AGLDPFHTRILKRLMRERRDKGTLIFITTHILTEIEELADTMLL"
BASE COUNT         1956 a         1496 c         1427 g         1803 t
ORIGIN      
        1 gcagtacgcc gggtttgcct tgagtgagcg acgctgagtg gttacccggg gtgagtgagc
       61 gtccccctgc ctctgcgcca ataagttcaa ctttgctgtc tttcaagaat gcagagaaca
      121 tgccgatcgc gtttgaacca ccgccgacac aagcgaacac cgcttgcggt aatttcccgg
      181 tagctttggt gaattgttgc cgtgcttcac ggccaattac agactggaat gtgcgcacca
      241 tcgtggggta ggggtgcggt ccgattgcac tcccaatgag ataatatgtg tcgtcgacat
      301 tttttgccca atcgcgcaat gcttcgttca ccgcgtcgcg caacgtgccc ttttcgccgc
      361 ccacaccaga gacggtcgca cccaaaagac gcatacgaaa atcgttgagt gattgccgac
      421 gcaaatcctc agttcccata taaatatgag cggggatttt cattaaggcg gctgcggtcg
      481 ccgtggcgac accgtgttgt cctgcgccgg tttcggcaat aatccgtcct tttttcattt
      541 tttgcgccaa aagcgtttgc cccaacgtgt tgttgatttt gtgcgagccg gtgtgcaata
      601 aatcttctcg cttcaaatac actcgcgcct gccacgccgc cgacaaattg ggtgcgtacg
      661 tcaacagcga cggtctaccc gcgtagtcat tgtacagatg caaaagttct ttttggaatt
      721 tagcgtcttt ggaaaatttt ttccacgcgg tattgagttc gtcgagcgcg ggtatgagca
      781 aatcggaaac gtagcgaccg ccaaaattac caaaatatcc ctcgggtggg gagattatct
      841 tctgtgaaga gtttgttttg ccgttttttg ctttcgcagc ggtcttttta ctcgccatgg
      901 tgtttccttt cgttacgaaa aattagagac attatgtcac attgtgaata actgtaaagc
      961 tcaaaaaagt gtatggagtc ctgcgagccc gtgagtcaac tcgctgagac aatttttttg
     1021 aaaatttttg tacacgctgc gcgagttggc acacagactc gtaactatct cgactgaaat
     1081 tttattacct tgaacttgac gtaaatcaat gtaattttta aaatcccttg agacaaaatt
     1141 gcacattctg tttgtggcat agagagtcgc cgcgctagtg gttaaatcta acccccaagc
     1201 atagtacata aaaggagctt ccaatgaaaa aaattttatt tctactcgcg gcaatatctc
     1261 ttgtcgcctg caacaaacca gaaggcaaca aagccaatgc taaacagcca gaacagggac
     1321 aatctaaagg tataggtcct gttcaatcgg tgactcttgc cggactcgat gcgggtcttg
     1381 ctcaaaaggg cgaaaaactt tttcatgaac aatgttctgc ctgccacaag atagacgaac
     1441 gctatgtagg ccctgcactc aaaggggtaa ctacccgccg cacacctgag tgggtcatga
     1501 acatgattgt aaaccccgaa cagatgacac gcgaagacgc caccgccaaa gaactcttgg
     1561 gtgagtatat ggcacaaatg actaaccaaa acgtggtcga agccgacgcg cgtatgatac
     1621 ttgagtactt tcgcaaagtg gatggtgtaa aataataaat actaaataga aattaatagg
     1681 ggggggaata cacagaacaa aatttttcta aactcaaaaa ttgaaattac ttttaaggag
     1741 aaaacaatga aaatgcaaat aaaacgcctt tctcttgtgg tggttgtggc aacgatggcg
     1801 atattcgctc tttcctgccg tggtaaaggc gccaaagatt tattggcaaa aaacgcagca
     1861 gagcgcgtct acgtcgctcc aggggacaag gatgaagtat acctctttac atcgggcggg
     1921 tttaacgggc aaattggagt gcaagggatt ccttcgggaa ggctcatcaa aacaattcct
     1981 gtcttttcgg tttggacaga aaatggttat ggcttcagcg acgaaaccaa acagatgctc
     2041 aacacatcgc atggttattt accgtgggac gatagccatc acatcgaagc gtcacaaacc
     2101 aaaggcaagc aagatggtcg gtggattttt atcaacggca acaacacccc ccgtattgcc
     2161 cgtatctctc tggaaagctt tgaaaccgaa gagattatcg aaattcctaa cagtgcaggc
     2221 aaccatgctt cgccattctt gacagagaac accgaatacg cgatggcagc cactcgtttt
     2281 tcggtaccga ttccacaacg cgacatcccc attgaaagtt atggcaaggg tgaatttcac
     2341 ggtaccatca ccatggttgg catcgatgca aaaaccggta agttgagctt aaaagggcaa
     2401 gtattggttc cgggttacga ttacgattta tcgcattgcg gtaaaggacc gtcgcacgat
     2461 tggtgctttt tcaccacgta caataccgag caagcgcata aaatgttaga gattgaagca
     2521 tcaaaaaacg acaaggactt tattcttgca tttaactgga aaaaagccga agcgtgtttc
     2581 aacgaaggta aaggaaaaac ggtttctgca aattatgtgc ataatatgcg cggtcacaac
     2641 agtaagctat ccacctctaa aaagtttact tcaatcaagt tgatgaatcc caaagattgt
     2701 gacaacatta tgtactacat gccaacaccc aaaagtccgc acggcgtgga tattgatccc
     2761 accggtgaat acattgtggg tggcggtaag ttagcgaccg taattcctgt tcactctttt
     2821 tcaaaactta ccaaagcgat tcaggacaaa gcaaatttca gcgaaactcg aatgggcatc
     2881 cccgtgctca agtacgaagc gactttggcg ggcgaggtac aaaaaccctg cttgggtcca
     2941 ttacacactg agtttgacgg caaaggctat gcctacacgt cttgctttgt gacctctgaa
     3001 attgtaaaat gggaattggg cacttgggaa gttaagcaaa acatgcccgc ctattatgcg
     3061 attggtcact tgagtattgt tggtggcgac tcagccaaac cttacggtaa atacttggtc
     3121 gctctcaaca aaatcactaa agaccgctat ttacccaccg gtccagagct tgtacaatct
     3181 gcacaacttt gggatatttc tggaagtaaa gcagagcttt tgttggattt ccccactcaa
     3241 ggcgagccgc actacgcgca aatgataccg gctgatttaa ttaaagataa aacacagaag
     3301 atttttgagc tgagcaagaa cgaacatcct tacgctgcaa aatctgaaaa agatgcaaag
     3361 gttgtacgtg aaggcaatgt tgtgcatgtg tatatgactg ctatccgctc acactttaag
     3421 cccgacactg ttgagctgaa agttggcgac actgcttact ttcatgtgac taacctcgaa
     3481 caagattatg atattcccca cggtttcgca atttacggcg caccgctacc caatatctta
     3541 atcatgccag ggcaaactct ctctgttaaa tacgaagcaa aacaattggg aatttttcct
     3601 ttttactgca ccgatttctg ctctgcatta caccaagaaa tgcagcagta cgttcgggtg
     3661 agtaaataaa ataaaaattg aacattactt gggagcctcg cccccaagta atgtaaaatt
     3721 aaagaggcaa caatgaaatt tctaaaaaca acaatccaac cgttacacaa aaaaatgctc
     3781 atcgctattg cagcgctcat gggtattgtg tattttatcc ctatatggca cattagtctt
     3841 gtggcgcctc aataccccga aggattgagc atgaacattt ttgtccactc gattaaaggc
     3901 ggtaatgcgc acgatttaac aaatatcaat ttactcaatc actacgtcgg catgcacgaa
     3961 attatcgaag agtctatacc tgagttgggg tttatgccgt acgtactggc gtttatgatt
     4021 ttaggcgcag tggtgacttt ttttcatgcg cgcatttaca tgttaatttt aggcgtgctc
     4081 aatttatttt tggtcgcttc tgcagggctg ttcgatttct ggcgttggga gtataactat
     4141 ggtcacaact taaatccgaa tgcacccatt attgtacccg gtatgacgta ccaaccgccg
     4201 cttttaggct gcaaaacgct gttgaatatc gaagcgtgca gctggccgca catcggagcg
     4261 attcttcttt ttatttccgt agccatacta ctcgttattt tataccgcga gaaaaaatta
     4321 aatagcggtg cataatgagc attatacgct tttcaattat tctcgttttg ttgactgtcg
     4381 cacaatgcaa tgtgccagaa cccgttgaaa ttaaagtcgg cagtcaacaa tgcgctcact
     4441 gccgcatggg aattgtagac atgcgcttta acgctcaact tgtaacaaca aagagtaaac
     4501 gctatcactt tgacagcatc gagtgtatgg aagaatggaa ggcagaacat tcagcgatta
     4561 aaatacgacg tgcatttgta aaaaataccg ctgccgttaa tttatatatc gactatcttg
     4621 atgcacgctt tgttcaatcg gaacgtttac cttcgccgat gggcgcttat ctcgccgcat
     4681 acgcaaccga agacgaagtt aaagaagcca tccgtgcgtt cggtgggcgc gaaattcaac
     4741 ggagtgctct tttaacggca tctaaaaatg cacaaacaca acaataggta aaatgtatcg
     4801 ttcaagaaga tttttttttc ataaagtatt ttttttatct caagtgggat ttctctcgct
     4861 actgacgcca tgccttgagg cgaaaacaat cacagtttgt tccacatgcc aatatatttc
     4921 tcctaaaact gcgattgacg gtgcgcagca cggcgacacg attatttttg acggcggcat
     4981 ttataacgta ggtacactta cagtttcaaa aaggctaagc ctcgtcggta aaaaaaatcc
     5041 gatttttgac ggtgaaaaca aaggccatat attagactta cgcgcagata acatcaccat
     5101 tgaaggtatc actctcaaaa atacgggagt gagcgacctt aaagagtttg ccgccattca
     5161 tgccgagtcg ctcaatcatt gttaaatcag aaaaaatatt ttttacaacg ccacttatgg
     5221 catttatttg gcacgtagcc acaattgctt catcgaagaa aatatttcgc atggcaatgc
     5281 gcaaaatgaa attttgggcg gcaacggtat tcatctttgg tatagcgacg ataatacaat
     5341 tagacaaaac gtcctcaaag gccaccgtga cggtatatat tttgagtttt cacaaaacct
     5401 ccacgttgaa aaaaatgttg caaccgagaa tattcgatac ggtctacatt ttatgttttg
     5461 tcacaattca atcattactg aaaatcgata tttcatgaac gcaaccggtg ttgcgcttat
     5521 gtactcaaaa aacttgaacc tcaccgccaa cgaatttagc gactctagag ggccgagtat
     5581 gcaaggtatt ttactcaaag acatcacaga cagcactgta caaaaaaaca aaatagcgca
     5641 caacacaact ggtcttttag ccgataactc atcgcgtaat aaaattcaac aaaatacctt
     5701 tgttatgaat ggccgcgcac tcgaagtatt tggtaacgca caagataatc gttttgaaaa
     5761 caatagtttt ttacaaaata tattcgacgt tgccgttaac tccaaagaaa ataccaatac
     5821 ctacgaaaaa aattactggg acaagtataa aggttatgac ttaaaccacg atggtatagg
     5881 cgacgtctct ttccaccctg tgcaaatttt tagtttttgg gtttcaaaat atccagagct
     5941 ctctatttta gccggctcgt cgatgattta ttttttagaa atcatcgaaa gagcatttcc
     6001 cgtgctcact ccgacttcgc tgcgcgacat gcgtccttta atgcaaaaac caatttatac
     6061 gagaatgtga tggcaaattt tttaacttta gaaaatatcc gtaagacata cctcaaaagg
     6121 cgtcgtgtac tcgaagacat atcgctcact cttcctcaag gacaaattgt tgctttagtc
     6181 ggacctaacg gttcgggaaa aacaacgctt atgaaaattc ttatggggct cacccacgca
     6241 gatgttggaa gcagtcttta tttggatggc gaaaaaactt tgttcgattt acccagccgt
     6301 atttccgttg gttatatgcc acaaaaccca ctgtttcccg acaacttacg cgtgcgtgaa
     6361 attatcgaat atatccgagg tttttctcag aacattcctc tctattatga cgcacttttt
     6421 accgagctta atgttgcatc ttttcacgaa aaaagattcc gcgaattgag tggaggcatg
     6481 cgccaaaaac tcagtgtctt gcaggcgttt atgtacgaac gcgctctgtt agttttagac
     6541 gaaccttcgg cgggtttaga cccctttcac acacgaatct tgaaaagact catgcgcgaa
     6601 aggcgcgata aaggaacgct tatttttatt actacgcata ttctcactga aatcgaagag
     6661 ctcgccgaca cgatgctttt gc
//
LOCUS       DAOMPD010000080         6660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4936_length_6660_cov_2.451022, whole genome shotgun sequence.
ACCESSION   DAOMPD010000080 DAOMPD010000000
VERSION     DAOMPD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4936_length_6660_cov_2.451022"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..648
                     /locus_tag="PLY93_04035"
     CDS             121..648
                     /locus_tag="PLY93_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68678.1"
                     /translation="MKKKVLWNYLFLGFLSITSFLFCRRGNPTNEAKIIPRLTNIALP
                     QTGCNNILEYARKTFTAEGDEVRVYDNPTKKGPYHWKQKFKEGVIAEGYGSVGRGTIT
                     LTLPIESTEPAFEICRRCTSFFHALTYQSFKDQNEVLQECGGRTKQGGCLVIWSIETV
                     RTKDGVRLKCGPESM"
     gene            complement(649..1131)
                     /gene="purE"
                     /locus_tag="PLY93_04040"
     CDS             complement(649..1131)
                     /gene="purE"
                     /locus_tag="PLY93_04040"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRP68679.1"
                     /translation="MLPRVAIIMGSKSDWSVMCAAQEILTDLGVVSHTEVVSAHRTPE
                     KMVAFAKSAKANGFHIVIAGAGGAAHLPGMVASLTPLPVIGVPVKSSNSLDGWDSILS
                     ILQMPAGVPVATVALNNAKNAGLLAAQILALADAQLSERLTTYRETQTQAVLQNSKIE
                     "
     gene            1187..1723
                     /locus_tag="PLY93_04045"
     CDS             1187..1723
                     /locus_tag="PLY93_04045"
                     /inference="COORDINATES: protein motif:HMM:NF022605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HRP68680.1"
                     /translation="MSAIARYIGSFFGSLAERIFVFFMALFISQGPQYMNLYLNVLSG
                     ATVEAEKSIKLIESKAGELGMTVPQFIEDLVKSQSQAAMKSGEIHQAQLKRFESLKAA
                     FSSIKNAGAFSRPFTFLKHVDWTLAKSVQFQPALPFTAEALGYVIAGILLGMLLFRVL
                     LFLPKKLLGGGKKIQGPL"
     gene            complement(1904..>2283)
                     /locus_tag="PLY93_04050"
     CDS             complement(1904..>2283)
                     /locus_tag="PLY93_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRP68681.1"
                     /translation="EQAAATPATPTAASAGQVISKVISREDVNKNILGNPAKIYEGAQ
                     FGPYLVNGKIEGYKIARVNDDHIFAKLGARSGDIIRKVNGYGLNDTERMFELWKAIRT
                     APEVKIELERDGKIITYDFQIRN"
     assembly_gap    2284..2319
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2320..2917)
                     /locus_tag="PLY93_04055"
     CDS             complement(<2320..2917)
                     /locus_tag="PLY93_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68682.1"
                     /translation="MALAAINNINVQELKKRADQAKEALLSLTARIRYSRTWWYTGAA
                     VLLGFALARMTHSTVLYFVTKSAPLPLARTSSQPTQQGEQIFTDPTITVGGALFQQAN
                     AAAQTQAGATVEQPAKPFKLTATLEGPIDIARALIEVQGEGTREYCAASSACRKKDKE
                     CECVVQNATIISIAQEYIWIKMNQSRHKLKIGQSTTDLA"
     gene            3061..3834
                     /locus_tag="PLY93_04060"
     CDS             3061..3834
                     /locus_tag="PLY93_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68683.1"
                     /translation="MSHKRMRRFSIRFYVTCLSCVAFTVLISCKKAPPINYLAPLDRT
                     QEGFLDPTTYQIVSYGYALDLTKPFDIATTFVPMSVNDTFDQEAFTDFNTQQAKLIRE
                     RKPTNGLLLAGVLTTEANFLHPQELDISALDEAIRAPMEIKRVFFDNACTHARIVGLY
                     RWVLTDVTHSKLLHGATLPNEAIQKTGLDKNYFPPRNFYIAESKNILKNLDAEMSQRN
                     YRYEIIQETFSKPEKLECRVVLHIHKKKLQLSMPFLAPP"
     gene            3842..4675
                     /locus_tag="PLY93_04065"
     CDS             3842..4675
                     /locus_tag="PLY93_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP68684.1"
                     /translation="MIAFDYKEIQAQAIGRGGIETCYMLPRLQIAFDVGRCPDALVDI
                     PKIFLSHGHLDHSAGLPYYISQRSLKHLPPPEIYCPAEIVEPLSEILKLWQKIEGFTY
                     TIQLTGLNAGDEIPIRKDMRVRALKAHHRVPCRGYAIIRRSKKLKEEFLTNSGREIQR
                     MKDRGDDIFDERDIPIFAFSGDSTIEFLLENKEAREAEVLFHECTYIDDKRPVSGARE
                     WGHTHLDEIIERQELFGNEKIVLTHFSKRYTRRFIQQVLEKKIKNKDFLNRIFVLAHG
                     S"
     gene            4665..5414
                     /locus_tag="PLY93_04070"
     CDS             4665..5414
                     /locus_tag="PLY93_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRP68685.1"
                     /translation="MVVNNKVKIKYSPKRARTVALKLTAMGQFELSVPVNTTDKWIQN
                     FLTKRQEWMQQAQEKAHAKKTQKQIPHNSRIETPHYSLAIFCDDTLQYPNYRVLRNNV
                     ENTTNFYLPSQFFEEKAQLLLHKRLEKYLLTRLMQVAGHTLIERAMYCSKRYKLNAKE
                     FFVRVQKSRLGYCTHDDRIMLNARLLLMPQKLIDYVICHELAHTRHRNHSKNFWSFLE
                     TLYPNAKVVDRKLRDASLYSMPKAKKIHTPH"
     gene            5417..6034
                     /locus_tag="PLY93_04075"
     CDS             5417..6034
                     /locus_tag="PLY93_04075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRP68686.1"
                     /translation="MKIYFGTENKSKAQEVELLFKAQAPAFKLLSIADLPETLRGKYN
                     AEEVGATYAQNALIKAQALFRLLDKNSFDYILAEDSGFEVDALGGAPGLHSARYAAND
                     DERCKKILAAIEGNPTALRRARFIACVALLKNGNDPVYFFGRKEGTVATALYGSDGFG
                     YDPIFIPEGGTKTWGEAGKGIKNTDSHRSRAFSLAAQYILSVVSQ"
     gene            6051..6494
                     /gene="smpB"
                     /locus_tag="PLY93_04080"
     CDS             6051..6494
                     /gene="smpB"
                     /locus_tag="PLY93_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRP68687.1"
                     /translation="MEIAVNRKARFNYEILEDIEAGIALQGTEVKSLRQIKVNISDGY
                     VVIRGEGVELINCRIEPYEQGNIMNHDPLRARRLLLKKKEIDRLRGKMKERGYALLPL
                     KMYFKGRYVKLLIGLGKGKKSHDKRQSIREKDIRRETEREMKNYR"
BASE COUNT         1791 a         1492 c         1610 g         1731 t
ORIGIN      
        1 cgcacaggtt tgggctgtca tgctttatag ggctccttcg ggagcctttt tagttgacca
       61 atcgtcacac tgttttgata ctaatagaat gttacataga aaatatagag ggtgtagaga
      121 gtgaaaaaaa aagttctatg gaattattta tttttaggtt ttttgagtat cactagtttc
      181 ctcttttgta ggagaggaaa tcctaccaat gaagcaaaga taataccgcg cctgacaaac
      241 atcgccttgc cgcaaactgg ctgtaataat attttagagt atgcacgcaa aacgtttacc
      301 gcagaggggg atgaagtgcg ggtgtacgat aatcctacaa aaaaaggccc ctaccactgg
      361 aagcaaaagt tcaaagaggg agtgattgcc gaagggtatg gttcggtggg gcgtggcacg
      421 attacgttga cgttgccaat agagagtacc gaacccgcgt ttgaaatttg ccgccgctgc
      481 acatccttct tccacgcgtt gacgtaccag tcgttcaaag atcaaaatga agtgttgcaa
      541 gaatgtggtg ggcgcaccaa acaaggcggc tgtcttgtta tatggtcgat tgaaaccgtg
      601 cgtaccaaag acggcgtacg tctcaagtgc ggccctgaat cgatgtagct attcaatttt
      661 tgagttttgc aaaaccgcct gcgtttgcgt ttcgcggtag gtggtcaatc gttcagagag
      721 ttgtgcatcc gctaacgcta aaatttgtgc ggcgagcaac cctgcattct tcgcgttgtt
      781 caacgcaacc gtcgccacag gaactcctgc gggcatttgt aagatagaaa gaatgctatc
      841 ccaaccatcg agcgagtttg aagattttac tggcacaccg atgacaggaa gtggggtgag
      901 tgacgcgacc atgccgggta aatgcgctgc accacctgcg ccagcgatga cgatgtgaaa
      961 accgttggct tttgcgcttt tggcaaatgc caccattttc tctggcgtgc ggtgtgcgga
     1021 gactacttcc gtgtgtgaca caacccccaa atcggtgaga atctcttgtg cagcacacat
     1081 tacagaccaa tcggattttg aacccatgat gatagcgact cgaggtaaca tgactcagga
     1141 agcacaaaaa atttgacccg ccacgtcacc cacgaattta tgtagaatga gtgcgattgc
     1201 aagatatatc ggtagttttt tcggttcgtt agccgagcgg attttcgttt tctttatggc
     1261 tctttttatc agccaaggcc ctcaatatat gaatctttac ctcaacgtgc tttcaggggc
     1321 aaccgttgag gctgaaaaat cgataaagct catcgaatcc aaggcggggg agctgggtat
     1381 gacggtgcca cagttcattg aagatcttgt aaaatcgcaa agtcaagcgg caatgaaatc
     1441 gggagaaatc catcaggccc aactgaaacg ttttgaaagc ttgaaagcgg cgtttagctc
     1501 gataaaaaat gcgggcgctt tttcgcgccc ctttacattc ttaaaacacg ttgactggac
     1561 tctcgcgaaa agcgtgcaat ttcaaccggc attaccgttt actgcggagg ctttgggata
     1621 tgtcatcgcc ggtattttgt taggaatgct tttgtttcgg gtgttattgt ttctgcctaa
     1681 gaaattattg ggcggtggca aaaaaatcca ggggccttta taaattgtac cctcttaagt
     1741 agcgaggata actatccagt attatgtcgc attgagggat aactatccag tattatgtca
     1801 cattgatgga taactataca cccccaagtg ctggaatggt aaattttgag aaaaagctac
     1861 ctttgtcaaa aaaatcgggc gtggctggac ggctgttggg tgcttagtta cgtatctgaa
     1921 aatcgtatgt aataatttta ccgtcgcgtt ccagttcgat tttcacctcg ggggcggtac
     1981 gtattgcttt ccatagttca aacatgcgct cggtgtcgtt taatccatag ccgttgactt
     2041 tacggatgat gtcgccgctg cgcgcgccca gctttgcaaa aatatgatca tcgttgacgc
     2101 gtgcaatttt atagccttca attttaccgt tgacgagata ggggccgaac tgcgcgcctt
     2161 cgtaaatttt tgcagggttg cctaaaatat ttttattcac gtcttcacga gaaatcactt
     2221 ttgaaatcac ttgacccgca ctggccgccg tgggtgtggc gggtgtcgct gccgcttgtt
     2281 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgctaagtcg gtggtggatt
     2341 gaccaatttt taatttgtgt cggctctggt tcattttgat ccagatgtat tcttgcgcga
     2401 ttgaaataat cgtggcattt tgcaccacac actcgcattc tttatctttt ttacgacacg
     2461 ccgaggaagc ggcacaatac tcgcgcgtgc cttcgccttg gacttcaatg agcgcgcggg
     2521 cgatgtcaat tggtccttcg agagtcgcgg tgagcttaaa aggctttgcc ggttgttcaa
     2581 cggttgcacc tgcttgggtt tgcgcagcgg cgttggcttg ttgaaagagc gcgccaccca
     2641 cggtgatggt ggggtcggta aaaatttgct ccccttgttg tgtgggctgc gacgaagtgc
     2701 gggcaagggg taacggtgca gacttagtga caaaatagag cactgtcgaa tgggtcatgc
     2761 gcgctaacgc aaatcctaaa agtaccgcag ctcccgtata ccaccaagtg cggctgtaac
     2821 ggatgcgcgc ggtgagtgaa agcaatgctt ctttggcttg gtcagcccgt tttttcagct
     2881 cttgcacatt gatattattt atagcagcca gtgccataac gcttagtcgg atgtgggatt
     2941 ttcaacgtca agcagaacag cgcggagaaa ttgactcagc gagttggcgc acaggctcct
     3001 cgccgcacag gctcgcacag actccaagtt taggttgccg cataaactca ttgatttatt
     3061 atgtcgcata aacgtatgcg gcgattctct attcgattct atgtaacttg tctcagttgt
     3121 gtagcattta ctgttctgat ttcatgcaag aaagcgcccc ctataaatta tctcgcgccg
     3181 cttgaccgaa ctcaagaagg attcttagat ccgacgactt atcaaattgt cagttatggt
     3241 tatgctctcg atttaactaa accatttgat attgccacta cgttcgtacc gatgagcgtc
     3301 aacgacacat tcgatcaaga agcgtttacc gattttaata cacaacaagc aaagttaatc
     3361 cgcgagcgta aaccgaccaa cggtcttctt ttggcgggcg ttcttacgac cgaagcgaat
     3421 tttttacatc cacaagagtt ggatatcagc gctttggacg aagcaatacg tgctcccatg
     3481 gaaattaagc gcgtcttttt tgataatgcc tgcacacatg cgcgcattgt tgggctctac
     3541 cgttgggtgc tcaccgatgt aacgcattca aaattgttgc acggcgcaac tttacccaac
     3601 gaagccattc aaaaaacggg attggataaa aattattttc caccacgcaa tttttatatt
     3661 gcagagtcga aaaatatact caaaaattta gatgcagaaa tgagccaacg taattatcgt
     3721 tatgaaatca ttcaagaaac attttctaaa cctgaaaaat tagaatgcag ggttgttctg
     3781 catatccaca aaaagaaatt acaactctcg atgccttttt tagcccctcc gtagtagtgc
     3841 agtgattgct ttcgactaca aagagattca ggcgcaggcg attgggcgcg gaggaataga
     3901 aacctgctat atgctgccgc gtttgcaaat cgccttcgat gtgggcagat gtcccgatgc
     3961 gttggtcgat attcctaaaa tttttttaag ccacggtcat ttagaccatt cggcgggact
     4021 gccgtactac atcagccagc gcagtttaaa gcatttgccc ccacccgaaa tttattgccc
     4081 cgctgaaatt gtcgaaccgc tttcggagat tctcaagctg tggcaaaaaa ttgaaggttt
     4141 tacgtataca atccaactca cggggttgaa cgctggcgat gagataccaa ttcgcaaaga
     4201 tatgcgggtg cgcgcgctaa aggcgcacca tcgtgtgcct tgtcgagggt atgcgatcat
     4261 ccgtcgcagc aaaaaactta aagaagaatt tttgacaaac tcagggcgtg aaatccagcg
     4321 gatgaaagat cgtggcgacg acattttcga cgaacgcgac attcctattt ttgctttttc
     4381 gggggattcg acgattgagt ttcttcttga aaataaagaa gcgcgcgagg cagaggttct
     4441 tttccacgag tgtacgtaca tcgacgacaa gcgaccggta tccggtgcgc gtgagtgggg
     4501 gcacacgcat ttagacgaaa ttatcgagcg gcaggaattg ttcgggaatg aaaaaattgt
     4561 gctcacccat ttttcaaaac gatatacccg tcgctttatt cagcaagtgc tcgaaaaaaa
     4621 aattaaaaat aaagatttct taaatcgcat ttttgttctc gcccatggta gttaataata
     4681 aggtaaaaat aaagtactcg ccgaaacgag cgcgcactgt tgcactaaag ctcactgcga
     4741 tggggcagtt tgaactttcg gtgccggtga ataccacgga caaatggatt caaaattttt
     4801 taaccaaacg tcaagaatgg atgcaacaag cgcaagaaaa agcgcatgcg aaaaaaacac
     4861 aaaaacagat accgcacaac agccgcatag agacaccgca ttattcgctt gctatttttt
     4921 gtgacgacac gctgcaatac ccaaactacc gtgtgctgcg caacaatgtc gaaaacacga
     4981 ctaactttta tttaccttct caattttttg aagaaaaggc gcaactacta cttcacaagc
     5041 gtttagaaaa gtatttgttg acgcgtctca tgcaagttgc ggggcacacg ctcatcgaac
     5101 gggcaatgta ttgctcgaag cggtacaagc taaacgcgaa agaatttttt gtgcgtgttc
     5161 aaaaatctcg attgggatat tgcacgcatg acgaccgcat catgcttaac gcgcgcttgc
     5221 tgttgatgcc gcaaaaactg atcgactatg tcatttgtca cgaactcgcg cacacaagac
     5281 accgcaacca ttctaaaaac ttttggagct ttttagaaac gctgtacccc aacgcaaaag
     5341 tcgttgaccg taagttacgc gacgcatcgc tctactcgat gcccaaagca aaaaaaatcc
     5401 atacgccaca ttgagtatga aaatttattt cggcacagaa aacaaaagta aggcgcaaga
     5461 agtcgaattg cttttcaaag cacaagcgcc tgcgtttaaa ttgctgtcga ttgccgattt
     5521 accagagacg ttgcgcggaa aatataacgc cgaagaagtc ggcgcgacgt acgcgcagaa
     5581 tgcactcatt aaagcccaag ccttgttccg tctcctcgat aaaaatagtt tcgattatat
     5641 actcgccgaa gattctggtt ttgaggtcga tgcgttgggt ggtgcgcccg ggctccattc
     5701 cgcaaggtat gcggccaacg acgatgagcg ttgtaaaaag attctcgccg ctatcgaggg
     5761 aaatcccact gcgctgcggc gtgctcgttt tatagcgtgc gttgcattgt tgaaaaatgg
     5821 caacgatccc gtttatttct ttggacgcaa agaaggcacc gtagccactg cgctgtacgg
     5881 tagcgatggc tttgggtacg accctatttt cattcccgag ggaggaacaa aaacttgggg
     5941 cgaagcgggt aaaggaatca aaaatacgga cagccaccgc agtcgcgcat tttctctcgc
     6001 agcgcagtat atactttccg tcgtgtctca gtagcggcgt ttaaccgggt atggaaatcg
     6061 cggttaacag aaaggcacgg ttcaactacg aaatcctcga agatattgaa gcgggtattg
     6121 cactgcaagg taccgaggtg aaatcgctgc gccaaataaa ggtgaacatc tccgacggtt
     6181 atgttgtgat acgtggcgag ggcgtggaac ttatcaactg ccgcatcgag ccttacgagc
     6241 aaggtaatat tatgaaccac gaccccttac gcgcgcgacg acttctatta aagaagaaag
     6301 aaatcgatcg tttgcgcgga aaaatgaaag aacgtggtta tgcgcttttg ccgctcaaga
     6361 tgtatttcaa agggcggtat gtaaaattac tcattggtct tggcaaaggc aagaaatcgc
     6421 acgacaagcg gcaatcgatc cgtgaaaagg acatccgccg tgaaaccgag cgcgaaatga
     6481 aaaattatcg ataggctggc gagctctcga caagttcttt gcctcttgtc tcacaatctg
     6541 gaaattcccc catgattgat tatcagctct tgaagagcga cgaaggtttt gcgcgtgtga
     6601 tgcaaagtct tcggcgccgt ggcgacacgc tttcaaccag cgctctcaaa gaagccgctg
//
LOCUS       DAOMPD010000081         6658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4938_length_6658_cov_2.648342, whole genome shotgun sequence.
ACCESSION   DAOMPD010000081 DAOMPD010000000
VERSION     DAOMPD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4938_length_6658_cov_2.648342"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PLY93_04085"
     CDS             <1..1639
                     /locus_tag="PLY93_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRP68688.1"
                     /translation="PRLSLQWFIDINPLAEKATHAVKTGATEFYPKRWENLFFDWMEK
                     IEDWCISRQLWWGHQIPAWYCVGDDHCKLECKEPIVAVEKPDACPHCGCKNLKQDSDV
                     LDTWFSSALWPFSTLLPKEPVEIDKAWQEGEVPTSAILDNFYPTSTLVTGFDIIFFWV
                     SRMLMMGMHFMKKAPFKKVFIHSLVRDANRQKMSKSKGNVVNPLEKMDEYGTDAFRFF
                     IISIMPDGKDIIYDESRLKGYQAFCNKIWNTARYILMNTPESYAIPKDVPTLSAVDVY
                     VLEELNAAIEKCEKAIQNFHFAEYALTIYDFLWKTFCDQYIEMSKVALKSTAMKEGAL
                     YTLYTVFAAALKILHPVMPFITEELHSFLPDAKESDLLVVSSWPKVDGEIRKVAHKTQ
                     ASTQALLAIVAKIRQVRAELSVEPGAKITAYLTLADTTLVAFIQEHLAFLQSLARLGD
                     IHFAVPEAKMIRAVIKEGDVYLDLAASIDIEREKARIEKEIQTLEKGRMTSESKLASP
                     QFAERAPKELVDAEKGKVAEYNTRLDALAQMLNSLKG"
     gene            1636..2763
                     /locus_tag="PLY93_04090"
     CDS             1636..2763
                     /locus_tag="PLY93_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HRP68689.1"
                     /translation="MSVFKERTERKYQTINDPIKLGTMLKQYATGKVFYLKGFYEKIE
                     VKALEAREGTMLVIDSLRDIEGPVVFYHTFNKHMEIHGEVVEKLVGSQYKVRALAVRI
                     ATNERRSARFQMSSDLVVINSIRAARNTINASLFNVPTSVKIHLEKFQDRLKDFADEV
                     KVKIFDKSDEKTELVRKTLKILMLTDTQDIMSYIPEDSEGFIDYREHLNTEVGTVMEE
                     YRKKKIVSEMIVPIIYLGHDGASIPLGFIQLVSYEKKLGVDTALELKMLAFEMVDQMR
                     DSNTMLINKRQAVADISRGGLRLVFTDPELKKFLVHQKGFSFDVVFKLQQPITVFTEI
                     VWVGHTPSQDLAIGVNIKGFSSRKGESDRYHQYIDSLNRAS"
     gene            2772..3203
                     /locus_tag="PLY93_04095"
     CDS             2772..3203
                     /locus_tag="PLY93_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP68690.1"
                     /translation="MRAIEVVQIKKHPVPVVQQTDSVLTAVKKMLELQESGLLILTPK
                     GDLVGIVTEKDVMRLVAERYTLLNKIEVWEIMSKSLTTVDADTPLEAVLTIMGEKNIH
                     HLPLMKEGKPLGLISSDDIIRARLQKSEHEAEMLKEYIQKQ"
     gene            3215..4375
                     /locus_tag="PLY93_04100"
     CDS             3215..4375
                     /locus_tag="PLY93_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRP68691.1"
                     /translation="MPKEITHCILAERAAYTLAASSNILKKRIGGEIYFLFEENPQAL
                     YFGSIAPDLFYYDIRMPWQMRLKNSSESVSDLIHGEGGIDTIAHVFQMLSILRDARLA
                     SPLTGDKTFSRSARATLLLFTLGYLSHVAIDTLLHPIVYYFAGNYYAHDPKEKNAAQT
                     RHRVIETILDLYNLDSLGSDLKTYHVLKKMKLEKKERQWILGFYTLALLRAFPQKMEA
                     LFKKSFSESTSVYTHPLYTAAVRSYKKQILSNRMFHSQRLARWGGSFNRRRHDALSHH
                     SSLFYPARSYAEYTQGNRGNYFTIEDLRKCVNPTTQREIHLSQKSITQKVFARTHAFY
                     RAAWRYAQGEINEVEARRVLKGYNLNNGLVKTPTEAMRTFSPLALNGNFEYI"
     gene            4394..4969
                     /locus_tag="PLY93_04105"
     CDS             4394..4969
                     /locus_tag="PLY93_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRP68692.1"
                     /translation="MNIILGVSASVALYKACDLTRELTKAGHHVHIAMTRTAASWINP
                     ILFEALSGNAVYTDDSQGTYAATVSCTERNRDRQISASSGMAHIDIRKDADVMLVAPA
                     TADLIARAAAGRGDDIVAATLLSFSGPRYIAPAMNPFMYAHPATQKNLQTLKEYGYRI
                     LDSANGEAVCGDVGQGKMMAVADIIAALSPN"
     gene            4981..>5352
                     /locus_tag="PLY93_04110"
     CDS             4981..>5352
                     /locus_tag="PLY93_04110"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRP68693.1"
                     /translation="MAESHWLDTLKEKYATQLVQSPIRVRLIAHTEQPFNIAAASART
                     CYSSKGLLLPEDMQKSEKSSAVSHKVARATLKSGHHTTRQHAHFVFGLENVSRQLIWQ
                     VLHAHPYYNSEQVSQRYVPIKN"
     assembly_gap    5353..5389
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5390..6540
                     /locus_tag="PLY93_04115"
     CDS             <5390..6540
                     /locus_tag="PLY93_04115"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRP68694.1"
                     /translation="AWYALPLSLSQRVDVHAYHEKVFAAYQALIETLKPTVEEIYFSI
                     HKLKARNKEAWASDIQKRCMEVARYVMPVSTVAYLYHSVSALTLMRYARMLLWSGYEE
                     FIVLGIKMFELIQAVDADLFQEFPEPLKPPTRVYDTHAALQKNKAFDASLENRPAKLI
                     SSSLNAIRHLDTAFLKQALSPGENTLLGDVFYPGTLDETSRLLNHAHFTFLKKLSHTA
                     DSQEQRHRTLPGNRPHLAEQISLEEDFIEPKLLGESTAAQKIYRSILAENFELLRTLW
                     KEESIPREDITYLVPNAFPVRFMESGDLYNFYHKWKARLCYNAQEEIFHSAYAEVDAV
                     RKLWPDLGKFLGPPCFVREFLKPRCPEGDHFCGIKVWQLPFEEYNRVI"
BASE COUNT         1811 a         1603 c         1603 g         1604 t
ORIGIN      
        1 gccacgcctg tcgttgcagt ggtttatcga catcaatccg ctcgccgaaa aagcgacgca
       61 cgcggtaaag acgggcgcca cagagtttta tcccaagcgt tgggaaaatc tttttttcga
      121 ttggatggaa aaaattgaag attggtgcat cagccgccaa ctgtggtggg ggcaccaaat
      181 ccccgcgtgg tattgcgtcg gagatgacca ctgcaaactc gaatgcaaag aaccgattgt
      241 cgcggtagaa aaaccagacg cgtgcccgca ttgcggatgc aaaaacttaa agcaagatag
      301 cgatgttttg gatacgtggt tttcatcagc gctctggccc ttctcgacgc ttctacccaa
      361 agaacccgtg gaaattgata aagcgtggca agagggagaa gtgccgacga gtgccattct
      421 cgataatttt tatcccacat caactcttgt gactggcttc gatattattt tcttttgggt
      481 gtcgcggatg ctcatgatgg ggatgcactt catgaagaaa gcgccgttta agaaagtatt
      541 catccattcg ctcgtgcgcg acgccaatcg ccaaaaaatg tcgaagtcga aaggcaacgt
      601 ggtcaatccg ctcgaaaaaa tggacgagta cggcaccgac gcgttccgtt tttttatcat
      661 cagcatcatg cccgacggca aagacatcat ctacgatgaa agccgcctca agggatacca
      721 agcgttctgc aataaaattt ggaatactgc tcggtacatc ctcatgaata cgcccgagag
      781 ttatgcaatc cctaaggatg tgccgacgct ttcggcggtc gacgtttatg ttttagaaga
      841 gctcaacgcc gcgattgaaa agtgcgaaaa ggcaatccaa aattttcatt tcgccgaata
      901 cgcgctcaca atttatgatt tcctttggaa aacattttgc gaccagtata tcgagatgag
      961 caaagtggcg ctaaaaagca cagcgatgaa agaaggcgct ctctatacac tttatactgt
     1021 gtttgccgcc gcactcaaaa tactccaccc cgtgatgccg ttcattaccg aagagctcca
     1081 cagctttttg ccggatgcaa aagagagcga tttgctcgtc gtctcgtcgt ggccgaaggt
     1141 cgatggagag ataagaaagg tggcgcacaa aacacaagct tcaacccagg ctcttttagc
     1201 gattgtagca aaaattcggc aagtgcgcgc cgagctttcg gtggaacccg gagcaaaaat
     1261 tacggcgtat ctaacgctcg ccgataccac gcttgttgca tttatccaag agcatttggc
     1321 gtttttgcaa tcattggcgc gtttgggaga tattcatttt gccgtgccag aagctaaaat
     1381 gatccgtgct gtaattaaag aaggcgatgt gtatttagat ttggcagcca gtatcgacat
     1441 cgaacgcgaa aaagcccgca tcgaaaaaga aatccaaacg cttgagaagg ggcggatgac
     1501 ttcggagagt aaacttgcct cgccgcagtt tgccgagcgc gcccccaaag agcttgtcga
     1561 tgccgaaaaa ggaaaagttg ctgaatataa tacccgcctt gacgctttag cacaaatgct
     1621 caattcgctc aaaggatgag tgttttcaaa gaacgcaccg agcgcaagta tcaaacgatt
     1681 aacgacccca taaaactggg gaccatgctt aaacaatacg cgacgggtaa ggttttttat
     1741 ttaaaaggct tttatgaaaa aatcgaagtt aaagcgttag aagcgcgcga aggcacgatg
     1801 ctcgtcatcg attcgttgcg cgacatcgaa ggtcccgttg tgttctatca cacgttcaat
     1861 aaacacatgg aaatccatgg cgaggtagtc gaaaagctcg ttggttcaca atacaaagtg
     1921 cgcgcgcttg cggtgcgtat tgccacgaac gagcgccgct cggcgcgctt tcaaatgtcg
     1981 agcgacttgg tggtgatcaa cagcatccgc gctgcgcgca acactatcaa tgcctcgttg
     2041 tttaatgtac ccacatcggt aaaaattcat ttagaaaaat ttcaagaccg cctcaaagat
     2101 tttgccgacg aagtgaaagt caaaattttt gataaaagcg acgagaaaac cgagttggtg
     2161 cgtaagactt tgaaaatttt aatgctcaca gacactcaag atatcatgtc gtacattccc
     2221 gaagactccg aggggttcat cgattaccgc gaacacctca acaccgaagt cggtacagtg
     2281 atggaagagt accgcaagaa aaaaattgtg tcagagatga tcgtccctat catttatttg
     2341 gggcatgacg gcgcgtcgat tccattgggg tttattcagc ttgtttctta tgagaaaaag
     2401 ctgggggttg ataccgcgct tgaacttaaa atgctggcgt ttgaaatggt cgaccaaatg
     2461 cgtgattcca ataccatgct cattaacaaa agacaggcgg tcgcagatat ttcacgcggc
     2521 ggtttgcgct tagtttttac cgacccagag cttaaaaagt ttttggtgca ccaaaaaggc
     2581 tttagcttcg acgttgtttt taagctacaa cagcccatca ccgtttttac ggaaatcgtt
     2641 tgggtggggc acacccccag ccaagacctt gcgatcggcg tgaatattaa aggtttttcg
     2701 tcgcggaaag gtgagagcga ccgttaccac caatatattg acagcctgaa tagagcatca
     2761 taaaaatggt tatgcgagct atagaagtcg ttcaaataaa aaaacaccca gtgccggtgg
     2821 tgcagcaaac ggattcggta ttgacggctg ttaaaaaaat gctcgagcta caagaaagcg
     2881 ggttgctcat tttaacgccg aagggcgatt tggttggcat tgtcaccgaa aaagatgtga
     2941 tgcgtttggt ggcggaacgc tatactctct tgaataaaat cgaagtgtgg gagattatgt
     3001 ccaaatcgct caccactgtc gacgccgaca caccgcttga agcggtgctc actatcatgg
     3061 gcgaaaaaaa tatacaccac ttgccgttga tgaaagaagg taaaccgttg ggacttattt
     3121 cctctgatga cattatccgc gcacgcctac aaaaaagcga gcacgaagcc gaaatgctca
     3181 aggagtatat ccaaaagcaa taacgcgctg gccgatgccc aaagaaatca cgcattgcat
     3241 tttagccgaa cgcgccgcgt atacgttggc ggcttcatcc aatattctta aaaaacgcat
     3301 cggcggcgaa atttattttc tttttgaaga aaatccgcaa gcactttatt ttggttcgat
     3361 tgcgccagac cttttttatt acgatatccg tatgccgtgg caaatgcggt taaaaaatag
     3421 cagtgaaagc gtgagcgact taatccacgg cgaagggggc atcgatacga tcgcccacgt
     3481 ttttcagatg ctctcgattc tacgtgatgc acgtttagct tctccgctaa cgggcgataa
     3541 aactttctcg cgcagtgcgc gcgcaacgct tttacttttt acgttggggt atttgtcgca
     3601 tgtcgcgatt gacacgttgt tgcaccccat cgtttattat ttcgcgggaa actattacgc
     3661 gcacgatccc aaagaaaaaa atgcagctca aacacgacat cgtgtcatcg aaaccattct
     3721 tgatctctat aatttagatt ctctggggag cgacttaaag acataccatg tactcaaaaa
     3781 aatgaagctc gaaaaaaaag agcggcaatg gattttgggg ttttatacgc ttgcgctttt
     3841 acgtgcgttc cctcaaaaaa tggaggcgct tttcaaaaaa tcgtttagcg aaagtacatc
     3901 tgtctataca cacccgcttt atacggcagc ggtgcgctcg tataaaaagc aaatactttc
     3961 taaccgtatg ttccacagcc aacgcttggc gcgctgggga ggctctttca accgccgccg
     4021 ccacgatgca ctcagccacc attcgagcct tttctatccc gcccgttcgt acgccgagta
     4081 cacccaagga aatagaggca attattttac gattgaagat ttacgaaagt gtgtaaatcc
     4141 cacaacgcaa agagaaatcc atctttctca aaaaagtata acacagaagg tgtttgcacg
     4201 cacgcatgcg ttttaccgcg ctgcgtggcg atacgcgcag ggtgaaatta acgaggttga
     4261 agcacgccgt gttttgaaag ggtacaatct caacaatgga ctcgtaaaaa cccccaccga
     4321 agcgatgcgt acgttttctc cgcttgcctt gaacggcaat tttgagtata tttgaggaaa
     4381 ttaaagaggt cgtatgaata ttattttagg agtgagcgca tcggtggcac tttacaaagc
     4441 gtgcgacctc acgcgtgaat tgacaaaagc ggggcatcac gtccacattg caatgacacg
     4501 caccgccgcc agttggatta accccatcct ctttgaagcc ctttcgggga atgcggtgta
     4561 cacagatgat agtcaaggaa cttatgccgc gaccgtctct tgcacggagc gaaatcgaga
     4621 tcggcagatc tccgcctcca gtggtatggc gcacatcgac atccgtaaag acgccgacgt
     4681 gatgttggta gcgccggcga cagctgacct catcgcgcgc gcggcggcgg gtcgtggcga
     4741 tgacattgtc gcagcaacgt tgttgtcttt tagtggacca cgctatatcg cgccggcaat
     4801 gaatcctttt atgtatgcac accccgcgac gcagaaaaat ttgcagacgc tcaaagagta
     4861 tggctatcgc attttagatt cagccaacgg ggaagcggtg tgcggcgacg tggggcaggg
     4921 gaaaatgatg gctgtcgctg atattattgc cgccctctct ccgaattgaa ccatagtaac
     4981 atggcagaaa gccactggct tgatacactc aaagaaaaat acgcaacgca actggttcaa
     5041 tcgcccattc gagtgcgcct cattgcgcac accgagcaac cgttcaatat cgccgctgca
     5101 tcggcgcgca cctgttatag ttccaaagga ctgcttctcc ctgaagatat gcaaaagagc
     5161 gaaaagtcaa gcgcggtgag tcacaaagta gcgcgcgcaa cattgaagtc gggtcaccac
     5221 actacgcgcc aacacgcgca ttttgttttt ggattagaga atgtatcgcg ccagctcatt
     5281 tggcaggtgt tgcatgcaca tccgtattac aactcggagc aggtgtcgca gcgctatgtc
     5341 cccatcaaga acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgcttggtat
     5401 gctctccccc tctcgctttc gcaaagagtg gatgtccacg cataccatga aaaagtattc
     5461 gcggcgtacc aagcgcttat tgaaacctta aaacccaccg tcgaagaaat ttatttttcg
     5521 atacacaaac tcaaagcacg caacaaagaa gcgtgggcga gcgacatcca aaaacggtgt
     5581 atggaagtgg cgcgttatgt gatgccggtt tccactgtgg cttaccttta ccacagcgtg
     5641 agtgcgctca cgctcatgcg gtatgcgcgc atgcttttgt ggtcgggata cgaggagttc
     5701 atcgtactcg gaatcaaaat gttcgagctc atccaagctg tcgatgctga tttgttccaa
     5761 gagtttcccg agccgttaaa accgcccacg cgggtgtacg acacccacgc cgcgctccag
     5821 aaaaataaag cctttgacgc atcgcttgaa aatcgccccg caaaactcat ttcttcttct
     5881 ttaaatgcca tccggcattt ggatacagcc ttccttaaac aggctctctc cccgggggag
     5941 aacactcttt tgggtgatgt gttttatcct ggcactctcg atgaaacgag ccgcctttta
     6001 aaccacgcgc attttacttt cttaaagaag ctctcgcaca cagccgactc gcaagagcaa
     6061 cgccatcgca cgctgccggg taacaggcca cacttggccg agcaaatttc tctcgaagaa
     6121 gattttatag agcccaaact tttgggtgaa tctaccgcag cacaaaaaat ttatcgcagc
     6181 atccttgctg aaaattttga actcttgcgc acgctttgga aagaagagag tatcccacgc
     6241 gaagatataa cgtatctcgt gccgaatgcg tttccggtgc gctttatgga atcgggcgac
     6301 ctctataatt tttatcacaa gtggaaagcg cggctgtgct acaacgcaca agaagaaatt
     6361 ttccattcgg cgtacgccga ggtcgacgcg gtacgcaagc tatggccgga tttaggcaag
     6421 tttttagggc cgccgtgctt cgtgcgcgaa ttcttgaagc cccgctgccc cgaaggcgat
     6481 catttttgcg gcatcaaagt atggcagtta ccgttcgagg aatataatag agtgatataa
     6541 aatacaatgg gttagggtct tcgtggcgtg agaaagcggt ggcctgctcg tctgaaggac
     6601 agtttttcct caaaatttac cctccttttc gaccactcca gcacccgcgg cgtatagt
//
LOCUS       DAOMPD010000082         6654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4945_length_6654_cov_2.301864, whole genome shotgun sequence.
ACCESSION   DAOMPD010000082 DAOMPD010000000
VERSION     DAOMPD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4945_length_6654_cov_2.301864"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLY93_04120"
     CDS             complement(<1..385)
                     /locus_tag="PLY93_04120"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRP68695.1"
                     /translation="MSREVGFLFNNVLLLASLFIVFFGTMYPTISEAISGRRESVTAE
                     WFNRFMAPMGIIILYLTGLGPLLAWRVTKLKVLFKNLRLPALMAFVAAVLSFFIAGGA
                     ALEWNAARIMAIITFAGAAFVVTGVL"
     gene            complement(476..1543)
                     /gene="ccsA"
                     /locus_tag="PLY93_04125"
     CDS             complement(476..1543)
                     /gene="ccsA"
                     /locus_tag="PLY93_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRP68696.1"
                     /translation="MHIFASIVFNLMLATAGYAVLASILHIIQSRRVRLAQGASPVDA
                     LYATQALRTSLSSARSAVEMHLIAVSICVALLLYMLATADFSIVYVAEHVNLALPMFY
                     RLTSLWAGQAGSMLWWNFLVILFSYLAIRHIRRVEPVLEPYMIAILMTISLFFTAIGC
                     FAPSSDPFRIFQAGADVYAQPEGRGLNPLLQHWAMIIHPPILYLGYVAFAVPFAIAMS
                     ALLYSKKHNHANTEGQIQQSIGISQRWIKYVRRWTIFSWFFLGAGILLGGKWAYEELG
                     WGGYWAWDPVENASLMPWLTATAFLHSIIVEEKRGMLKVWNMVLVSITFIMTVVGTAL
                     TRSGVVQSVHAFAQSNLGGFF"
     gene            complement(1545..2519)
                     /locus_tag="PLY93_04130"
     CDS             complement(1545..2519)
                     /locus_tag="PLY93_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP68697.1"
                     /translation="MRLIYIFVLAFLVACSTTPENETPVRFDEIITQATQLSEKGSVD
                     EAKYARLVEALERIRRTAPQYKEDASSMLLAANFMKLFPSVTCELTLTEPNEPLYIDE
                     GFTTPLKVRATCPVKAPFAFEADSQYVELKGENFAYDDGKLTAELRVTSVNALREKNK
                     LRFAAHYYASPLLKLLREDFNSVDSSIIAINPVEYTIAQTPSNKENTVNVASRGVDKN
                     DIGVNPTQQAQVQLKVIFMKGSIQLSREARQKIADMRLKPGQKIRVTGFADAESKSKK
                     DKERIANLRARVVASFLNEAVGITDAAIEWKTASENDLASTALIEGVE"
     gene            2755..3972
                     /locus_tag="PLY93_04135"
     CDS             2755..3972
                     /locus_tag="PLY93_04135"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRP68698.1"
                     /translation="MQTPAIKKIVLAYSGGLDTSVILHWLKKTYNCEVVAYCADIGQE
                     EELSGLDEKAKNTGASKCYIEDLRGEFVKDYVFPMLRSSAVYEMRYLLGTSIARPLIA
                     KRQVEIAAKEGADAVAHGATGKGNDQVRFEMAFMALAPQLKIVAPWRTWNFKGREDLI
                     RYAQEEKIPVVASAAKPYSMDRNALHISYEGGVLEDPWHEYKEEMFILTQSAEKAPDT
                     AEVVTIDFTHGNATAVNGQKMEPFELLMHLNKLGGKHGVGRIDIVENRLVGIKSRGVY
                     ETPGGTILHLAHRDLESITLERNLQHTKDELSLKYARLVYNGQWFTPEREALQAFVDQ
                     TQRVVTGQVKVKLYKGSAIVLGRQSPHSLYSSALASFEKEDVYNQFDAEGFIRLFGLT
                     ARNSYAQYKKEIG"
     gene            3978..4433
                     /locus_tag="PLY93_04140"
     CDS             3978..4433
                     /locus_tag="PLY93_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68699.1"
                     /translation="MAKTQTFVSPENWYSLEYPRTWEMEVVEGIPSFFDGINPYGGVL
                     QLLAANLAGAANQELLTHSPFLAGKDLGEKLMRFLSKQGVSDEAARFFIRDNTEMGVC
                     EYRLDGHFYLTLMAERAKVFVLALYNCKGDPDGEEAKLVGEILQSLVLL"
     gene            complement(4462..5148)
                     /locus_tag="PLY93_04145"
     CDS             complement(4462..5148)
                     /locus_tag="PLY93_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68700.1"
                     /translation="MQTHDYILAGATGLVGTEILKQLAHESNAGQIICLGRTAPPHLP
                     PRGKFIAHDFKAHVDWQKLKLNAPVAICTLGTTIKKAGSQEEFRRVDYDYVLNFAEET
                     KRIGAIALHVVTAHGASSHSSIFHNRVKGEIEEKLKSLALPSLHIYRPSLLLGERHEK
                     RFSEGAATWAVKFLAPLFKLPGLNAVQPTPVSHLARYILHSAQNSATGNHVHSNSQMM
                     STTELNFEGG"
     gene            complement(5190..6575)
                     /gene="murD"
                     /locus_tag="PLY93_04150"
     CDS             complement(5190..6575)
                     /gene="murD"
                     /locus_tag="PLY93_04150"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRP68701.1"
                     /translation="MQNHVLLLGAGGKTGASYARLLQEHGHHVLWYDKNSNATPQGLT
                     PSLTTHIPHDALDFTRLKNNFSVLTLTPGVPLSHPFIQEARNAGKKIISEVAYVAPYL
                     INHTILGVTGTDGKSTTTTLTAQLLRALGHDAIECGNFGIPLSQIILEGEKYKKTILV
                     CELSSYQLEEPGDLKLNAGIFLNLAPDHLNRYASIEEYGLAKWNIASCLKKDAPLILV
                     KNLTVGFTPYWKNAHPLNHFTQPLLLVDTERLHAPHFHIEKDTLVFSSGEKSLTLDTL
                     KIQGRHNWGNLLFALEATQSVLKNPSVQFSPAIQTLNPLPHRFEIIPQTAFPQITFIN
                     DSKATTTQAALTALQNVKDTLFIFLGGQAKGERYHKLGERLKELEAHACIYGACQKEM
                     AADFESVSFTTFTLHENLFKAFHAAKRMVIQQQISRATLLLAPAVTSWDQFASFEERG
                     DYFRELVAALE"
BASE COUNT         1717 a         1551 c         1724 g         1662 t
ORIGIN      
        1 ctaaaacgcc agtgacgaca aacgctgcgc ctgcaaaagt gatgatcgcc atgatgcgtg
       61 cggcattcca ttcgagggct gctccccctg cgataaaaaa cgaaagaacg gctgccacaa
      121 acgccatgag ggctggcaga cgtaaatttt tgaaaagcac tttcagcttg gtgacgcgcc
      181 acgctaaaag tggccctaaa ccggtgaggt atagaatgat gatacccatc ggcgccatga
      241 agcggttgaa ccattcggcg gtcaccgatt cacgccgtcc tgaaattgct tcgctgatgg
      301 tggggtacat cgtgccgaaa aacacaatga agagcgatgc aagcaaaagt acgttattaa
      361 agaggaaacc cacctcgcgg ctcatgagcg attcaattgg cgccgacgat tttaaaatat
      421 cgtagcggcg gtagatgacc caggcgcaaa aaagaataat gaaaataacg aacgcctaaa
      481 agaaaccacc aaggttcgac tgtgcgaacg catgcaccga ttgcaccacg cctgaacggg
      541 tgagcgcggt gcccacgact gtcatgatga acgtgatgga aaccaacacc atgttccaca
      601 ccttgagcat gccccgtttt tcttcgacga tgattgaatg caagaacgcg gtggcggtga
      661 gccatggcat gagcgaggcg ttctccaccg gatcccacgc ccaatagcca ccccagccta
      721 actcttcgta cgcccattta ccacccaaca aaataccagc gcccaaaaaa aaccagctaa
      781 agatggtcca acggcgtacg tatttaatcc accgttgcga gatgccgatg ctttgttgaa
      841 tttgtccctc ggtgttggcg tggttgtgct ttttgctata aagcagcgcc gacatggcga
      901 tggcaaacgg cacggcaaat gcgacgtagc ctaaatataa aataggggga tggatgatca
      961 ttgcccaatg ctggaggagt ggatttaaac cacggccttc gggctgtgca tacacatcgg
     1021 cgcccgcttg aaaaatacgg aatggatccg agctgggcgc aaagcaaccg atcgcggtga
     1081 aaaaaagtga gattgtcatg aggatcgcaa tcatatacgg ttcgagcact ggctcaacgc
     1141 ggcggatatg acggatcgct aaataggaaa ataaaatgac gaggaaattc caccagagca
     1201 tcgagccggc ttggcctgcc caaagcgacg tgaggcggta gaacatcggc agcgctaagt
     1261 tgacgtgttc ggcgacgtac acaatcgaga agtcggcggt ggcgagcata taaagaagga
     1321 gtgccacaca aatagaaacc gcgataaggt gcatctctac cgctgagcgt gccgacgaaa
     1381 gcgacgtgcg taacgcttgc gtggcgtaaa gagcgtcgac gggagaagcg ccttgagcga
     1441 ggcgcacacg gcgcgattgg ataatgtgta aaatcgacgc gagcaccgca tagcctgcgg
     1501 tggcgagcat caagttaaaa acgatggacg caaaaatatg catgttattc aactccttca
     1561 atgagggcgg tgctggctaa atcattttca gacgccgtct tccattcaat ggcggcatcg
     1621 gtgatgccta ccgcttcgtt caaaaatgaa gccacaacgc gcgcgcgtaa atttgcaata
     1681 cgctctttat cttttttgct tttactttct gcgtcggcaa agccggtgac gcgaattttc
     1741 tgcccaggct ttaatctcat atcggcgatt ttttgccgcg cctcgcgtga gagttgaatc
     1801 gagcctttca taaaaataac tttaagctgc acttgcgctt gttgagtggg atttaccccg
     1861 atgtcgtttt tgtcaacacc gcgcgaggcg acattcactg tgttttcttt gttggagggc
     1921 gtttgagcga tggtatactc gacagggttg atcgcaataa tacttgaatc gacgctatta
     1981 aaatcttcac gcagcagttt taaaagagga gaagcgtaat agtgtgcggc gaaacggagt
     2041 ttgttttttt cgcgtagcgc gttcaccgat gtcacgcgta actcagcagt gagtttgccg
     2101 tcgtcgtacg caaaattttc acctttaagt tctacgtact ggctgtcggc ttcaaaagca
     2161 aacggcgctt taacaggaca ggtggctctc accttgagtg gggttgtaaa cccttcgtcg
     2221 atatagagcg gctcattggg ttcggtgagc gtcaattcgc atgttaccga aggaaaaagt
     2281 ttcatgaagt tcgccgctaa aagcatactc gacgcgtctt ctttatattg tggagccgtg
     2341 cggcggatac gttcgagcgc ttcgacaagg cgcgcatatt tggcttcgtc gacgctgcct
     2401 ttctcgctca gttgagtggc ctgtgtaatg atttcatcga agcgcaccgg tgtttcattt
     2461 tcaggggtgg tggagcacgc cactaaaaaa gcgagtacaa atatataaat gagccgcacg
     2521 agtacgtttt tagcgtttgg agacagagtg gcaataaagt tcacatattt aagagtctat
     2581 gcgccaactc gctgaagcaa ttcttttgca aaatttcgca tttttgaacg aaccgcgtat
     2641 aaaatttaca aaaaaattgt ctcttgtaca cgctgcgcga gttggcgcac agactcgtag
     2701 gaagcgcatc ttcacattca tttacgcgct atcgcaagtc ttctttgagg tatcatgcaa
     2761 actcctgcga ttaaaaaaat tgtccttgcg tactcaggcg ggctagacac ctctgtcatt
     2821 ctccattggc tcaaaaaaac atacaactgc gaagtggtcg cgtattgcgc cgacatcggc
     2881 caagaagaag agctctccgg cttagacgaa aaagccaaaa acaccggcgc gtcaaaatgc
     2941 tatatcgaag acttacgcgg cgagtttgtg aaagactatg ttttcccgat gctgcgctcg
     3001 tcggcggtgt acgaaatgcg ctatcttttg ggtacgtcga ttgcgcgccc actcattgcc
     3061 aagcggcaag ttgaaatcgc cgccaaagaa ggcgccgacg cggtggcgca cggtgccacc
     3121 ggcaaaggca acgaccaagt gcgttttgag atggcgttta tggcgcttgc cccacaactt
     3181 aaaattgtag cgccgtggcg cacatggaac ttcaaaggcc gcgaagattt aatccgttac
     3241 gcccaagaag aaaaaattcc ggtggtggct tctgccgcca aaccctactc gatggataga
     3301 aacgcgctgc acattagcta cgaaggcgga gtgctcgaag acccatggca tgaatataaa
     3361 gaagaaatgt ttatcctcac ccaaagcgcc gagaaagcac ccgataccgc cgaggtggtc
     3421 accattgatt ttacgcacgg caacgccacg gcggtgaacg gccaaaaaat ggagccgttc
     3481 gagctcctta tgcacttaaa caaactgggc ggcaagcacg gcgtggggcg catcgatatc
     3541 gtagaaaacc gccttgtcgg catcaagtcg cgcggtgtgt atgaaacgcc gggcggcacg
     3601 attttacatc tggcgcaccg cgacctggag tcgatcacgt tagagcgcaa cttgcagcac
     3661 accaaagacg agctgtcact caaatacgca cgccttgttt acaacggcca atggtttacc
     3721 cccgaacgcg aagcgttgca ggcgtttgtc gatcaaacgc agcgggtggt gaccggccaa
     3781 gtgaaagtca aactttacaa aggaagcgcg attgtgttgg ggagacaatc cccgcattcg
     3841 ctttatagtt ctgcattggc gtcttttgaa aaagaggacg tgtataacca attcgacgct
     3901 gaagggttta tccgtctttt cggactcact gcgcgtaatt cgtatgcgca gtacaaaaaa
     3961 gaaatcgggt aaaccgagtg gcaaaaacgc aaacatttgt ttctcctgaa aattggtatt
     4021 cgctcgaata cccacgcact tgggaaatgg aagtggtcga aggcatcccc agtttttttg
     4081 acggcataaa tccttatggc ggagtgttgc aacttttggc ggcaaacctt gcgggcgcgg
     4141 caaatcaaga gttgctcacg cactcgccat ttcttgcggg gaaagatttg ggtgaaaaac
     4201 tcatgcgctt tttatcaaag caaggagtga gcgacgaagc agcgcgtttt tttatccgcg
     4261 acaataccga aatgggcgtg tgcgaatata ggctggatgg ccacttctac cttacgctca
     4321 tggccgagcg ggcaaaggtt ttcgtgctcg cgctttacaa ctgcaaaggc gaccccgacg
     4381 gtgaagaagc caaacttgtg ggtgagattt tacaatcttt agtgttgctt tagaggtggt
     4441 taatggcttc ctcagcggcc attagccacc ctcaaaattt agctctgtcg tgctcatcat
     4501 ttgtgagttt gaatggacat gatttccagt agcagagttc tgcgcgctat gcaatatata
     4561 ccgtgcaaga tgcgagaccg gtgtgggttg cactgcgttg agtccaggta gtttgaaaag
     4621 tggcgctaag aactttaccg cccatgtcgc ggcaccttcg ctaaaacgtt tttcgtggcg
     4681 ttcacctaag agtaacgacg ggcggtaaat gtgcaaagat ggcagcgcta acgatttgag
     4741 cttctcttca atctctccct tgacgcggtt gtgaaaaatg ctcgaatggg acgatgctcc
     4801 atgcgcagtt actacgtgga gcgctatcgc gccgattcgt tttgtctctt cggcgaaatt
     4861 taacacatag tcataatcca cgcggcggaa ttcttcttgg ctgcctgctt tcttgattgt
     4921 cgtacccaaa gtgcatattg ccacaggcgc attgagcttg agcttttgcc agtcgacgtg
     4981 tgctttgaaa tcgtgcgcaa tgaattttcc ccgtgggggc aggtgtggtg gcgcagttct
     5041 acccagacaa atgatttggc cggcgtttga ttcatgcgct aattgcttta ggatttctgt
     5101 tcctacaagc cctgtagcgc cggctaaaat ataatcgtgt gtttgcattt tatgcgcctc
     5161 ttttattttt ttatgtttga aagatacact tattctaacg ctgcgactaa ttctctaaaa
     5221 taatcgccgc gttcttcaaa gcttgcaaat tgatcccacg aggtgacggc gggagctaag
     5281 agtaaagtag cgcgggagat ttgctgttga atgaccatcc gcttggcggc atggaatgcc
     5341 ttgaataaat tttcatgcag cgtgaatgtg gtaaaactca cactttcaaa atcggctgcc
     5401 atttcttttt ggcatgcgcc gtaaatacac gcgtgcgctt cgagctcttt aagccgttcg
     5461 cctagtttgt gataccgctc gccttttgct tggccgccta aaaagataaa caaagtgtcc
     5521 tttacatttt ggagcgcagt aagcgctgct tgggtggtcg tcgctttgct gtcgttgata
     5581 aaagttattt gcgggaaagc ggtctgtggg ataatttcaa atcggtgtgg taacggatta
     5641 agcgtttgaa tagcaggaga gaattgtacc gagggatttt ttaaaacact ctgcgttgcc
     5701 tcgagtgcaa aaagaaggtt tccccagtta tgcctacctt ggatcttcaa cgtgtcgagc
     5761 gtaagcgatt tttcaccgct gctaaaaaca agagtatctt tttcgatatg aaaatgtggc
     5821 gcgtgcaagc gttcggtgtc gacaagcaaa agcggttgtg taaaatggtt aagtggatgt
     5881 gcgtttttcc agtacggggt aaatccgact gttaaatttt tgacaagaat gagtggcgca
     5941 tcttttttaa ggcaggaagc aatattccac ttggccaaac cgtactcttc gatacttgca
     6001 tagcggttta aatggtcggg ggcaagattc aaaaaaatac cggcgttgag ttttaaatct
     6061 cccggctctt ctaactgata actcgaaagc tcgcacacca agatggtctt tttatatttc
     6121 tcgccttcta aaataatttg cgagagagga atgccgaaat taccacattc gatggcgtcg
     6181 tgtcccaacg cacgcaaaag ttgcgccgtt aaagtggtcg tggtgctttt gccgtcggta
     6241 ccggtgacgc cgagtatggt atgattgatc aagtaaggag ctacatacgc cacttcgctg
     6301 ataatttttt ttccggcgtt gcgcgcttct tggataaagg gatgactcaa aggcacaccc
     6361 ggggtcaacg tgagtacgga aaaattattt tttaatcgcg taaaatctaa cgcatcgtgt
     6421 ggaatgtgtg tcgttaaaga gggagttaat ccttgcggcg tggcgttgct gtttttgtcg
     6481 taccacaaca catgatgacc atgctcttgt aaaagtcgtg catacgatgc gcctgttttg
     6541 ccgcccgctc ccagaagtaa aacgtgattt tgcatattta aagaattagt gtttgtgcgg
     6601 tggcatctga tagcgctccc aaagtattat taaaagcggc tgccatataa ccgc
//
LOCUS       DAOMPD010000083         6648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4954_length_6648_cov_2.263765, whole genome shotgun sequence.
ACCESSION   DAOMPD010000083 DAOMPD010000000
VERSION     DAOMPD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4954_length_6648_cov_2.263765"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLY93_04155"
     CDS             complement(<1..253)
                     /locus_tag="PLY93_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68702.1"
                     /translation="MPKIMFYDKSQRNLVLSYTAPWRQANNMTRFYRSGGLFDKKYSK
                     RETAVGHEISLPKKWGPTRLKESFSSKFTLLFDHSSTRGV"
     gene            333..611
                     /locus_tag="PLY93_04160"
     CDS             333..611
                     /locus_tag="PLY93_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRP68703.1"
                     /translation="MVYNKLISDERINLVKRLNRISGQLEGLKKRVAAGESNCMSDMT
                     QIKAIRNALWKFAEAYVEAHIAECTSQKKSPKEIKQNLESVIQAAFSL"
     gene            637..804
                     /locus_tag="PLY93_04165"
     CDS             637..804
                     /locus_tag="PLY93_04165"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68704.1"
                     /translation="MKSPMYVIAGVVLGGLLGYLYYRFVGCKTGACPIVSKPVPSILY
                     GAALGGMIGGT"
     gene            806..>1023
                     /locus_tag="PLY93_04170"
     CDS             806..>1023
                     /locus_tag="PLY93_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRP68705.1"
                     /translation="MSVAKKIVIVGGVAGGATAAGRLRRLDENAEITLLEASSHLSFA
                     NCGLPYHIGGDIVDRNKLLLRTPEDFWKR"
     assembly_gap    1024..1081
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1082..2473
                     /locus_tag="PLY93_04175"
     CDS             <1082..2473
                     /locus_tag="PLY93_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68706.1"
                     /translation="ILASDLLVRYRIRAEVNATVEKIDTNTKTVSYRKNGTVKIISYD
                     KLILSQGGNPNKVAVEGIEHVPHFSLRLVEDMDAIIAQMQEPKSQRVIVVGGGFIGIE
                     LAEAFIQKKLNVTLVEFAPNVMANLDTEFSNLAKETLVVNGVDVRTRTTIQKVEAGGK
                     ITLSDGSTHTVDFVVFAAGVKPETTLATQMQLQIGKSGGVVVDEFMQTSNPDVYAVGD
                     MVEIKNRFTGLSTRIPLAGPANRQGRIAAENIVWGNKSRYAGALGSAVVKIFDTTLAF
                     TGLTEKAANELSIEAGTVTIHGNNHAGYYPNAERITLKLIFDTKDGRVLGATAFGKEG
                     TEKRIDVIATALVGKLTVYDLATLDLCYAPPYGSANDVVNVAAFQAINHLSKLSPVVT
                     PAALDANRADFNVVDVRISKERDECKTECQCAIEVDTLRECLSDVPCDKPVALLCQSG
                     QRSYIASRILKQK"
     gene            2539..2898
                     /locus_tag="PLY93_04180"
     CDS             2539..2898
                     /locus_tag="PLY93_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRP68707.1"
                     /translation="MTEQQIIWISALSLLLVFILYRKHKLGRNKVNVKGMLQQGAKVV
                     DVRSAGEFAGGHYKGAINIPLDALSSRLNTLGDKSKPVIVYCASGMRSGSAKRVLESA
                     GFTQVENGVNQGYLESL"
     gene            complement(2948..3514)
                     /locus_tag="PLY93_04185"
     CDS             complement(2948..3514)
                     /locus_tag="PLY93_04185"
                     /inference="COORDINATES: protein motif:HMM:NF020348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HRP68708.1"
                     /translation="MEEQDILKVFFGPLFPLIGTWTGDKGMDISPEPEGEEHSPYFET
                     ITIEPVGDAVNAEEQKLVVLSYKQVVCRKSNGEVFHHQLGYWYYEKKTNEIFYSLSIP
                     RAVSVLAKGNAQTKGNQVDFFVEAKKEDPNFGVLETPFMSAKASTRGFTLKVNVDGDT
                     MRYTMNTALSIYDRSFDHTDENTLTRKK"
     gene            3625..>5317
                     /locus_tag="PLY93_04190"
     CDS             3625..>5317
                     /locus_tag="PLY93_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRP68709.1"
                     /translation="MNKIRITGAREHNLKNVSLEIPREKLVVITGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSSYARQFLGQLEKPDVDSIEGLSPAISIEQKTTARNPRSTVGTVT
                     EIYDYLRLLFARLGDPHCPECGAKLEAQSIDTMVASVFGIFDRMPKSQSIRTQILAPI
                     ARSKKGEFKDVFERIQRDGFVRVRVDGQIKSLDEEISLKKNLKHDIDIVVDRLVLEKD
                     KLQEQRMHVAGSLELALKQAVTGGGSGGQASILFEGDGGEQLEEHFSAKLYCTSCELS
                     FPEITHRLFSFNSPDGACEGCSGLGHTLEFHPDKLIQNDEQTVREGIGEGLGFSADAY
                     WYKSSIEALDKKLHFGVDTPWCKLSKKVWELLLYGDKSLKLNYEWKGTESSYQYSRGY
                     EGLIPNLQRRYLQNASDSMRQKMEQYMEEMPCAVCKGKRLKPIALAVKLKGKNIAETT
                     ALTLETALEYFQKIQFNKTEREIAQQAMKEILDRLRFLNSVGVGYLTLDRIAGSLSGG
                     EAQRIRLATQIGSALMGVLYVLDEPSIGLHQSDNEKLIQTLKGLRDLGNSIIVVEH"
     assembly_gap    5318..5327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5328..6505
                     /locus_tag="PLY93_04195"
                     /pseudo
     CDS             5328..6505
                     /locus_tag="PLY93_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777410.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
BASE COUNT         1783 a         1474 c         1695 g         1628 t
ORIGIN      
        1 atacgccgcg ggtgctggag tggtcgaaaa ggagggtaaa ttttgaggaa aagctttctt
       61 tcaggcgggt agggccccac ttcttcggca gactgatctc atgaccgaca gcagtctcgc
      121 gtttactata tttcttatca aacaggccgc cactgcgata gaaacgtgtc atattgttag
      181 cctgtcgcca cggagccgta taacttaata ccaaatttcg ctgggatttg tcgtaaaaca
      241 tgattttggg catactttgt tatgtcgttt ggtttaggat gttagtaaga ttttttgcct
      301 ctacgtattt aaatttatat actatacagg gtatggtata taacaaatta atttctgatg
      361 agcgcattaa tttagtaaag cgcctcaatc gtatttcagg ccagttggaa gggctcaaga
      421 aacgtgtcgc cgcaggagag tcaaactgca tgagcgacat gacacaaatc aaggcgatcc
      481 gcaatgcgct gtggaaattt gccgaggcgt acgttgaggc gcacatcgct gagtgcacct
      541 cgcaaaaaaa atctcccaaa gaaattaaac aaaatttaga gtctgtcatc caagcggcgt
      601 ttagtttgta attttagaaa taaaaaaaga ggtaaaatga aaagtcctat gtatgtcatt
      661 gcgggcgttg tgttgggtgg tcttcttggt tatctttact accgctttgt cggttgtaaa
      721 actggcgcat gccccatcgt ttcaaagccg gtcccttcta ttttgtacgg cgcagcgttg
      781 ggcggcatga ttggtggaac ttaacatgag cgtagcaaaa aaaattgtta tcgtcggtgg
      841 tgtcgccggt ggggccactg cggcaggacg tttgcgccgc ctcgatgaaa atgcagagat
      901 tacactcctt gaggcgagca gccatctttc gtttgccaac tgtggcttgc cgtaccacat
      961 cggcggcgat attgtcgacc gcaataaatt actcctacgc actcccgaag atttttggaa
     1021 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 natcctcgcc agcgacctct tggtgcgcta tcgcatccgt gccgaggtga acgccactgt
     1141 agagaaaatt gacacgaata caaaaacagt atcgtacaga aaaaatggca cggtaaaaat
     1201 aataagttac gacaaactca ttttgtcgca agggggaaat cccaacaaag tggcggtaga
     1261 aggcatcgag catgtgccac atttttcgtt gcgccttgtg gaggatatgg acgcgatcat
     1321 cgctcaaatg caagaaccca agtcgcagcg ggtaattgtt gttggcggtg gttttatcgg
     1381 cattgagttg gcagaagcat ttattcaaaa aaaattaaac gtgacgctcg tcgaattcgc
     1441 gcccaacgtg atggcgaatt tggataccga attttccaac ctcgcaaaag aaaccctggt
     1501 ggttaatggc gttgacgtgc gtaccagaac cacaattcaa aaagtcgaag cgggtggcaa
     1561 aattactctc tccgacggtt cgacgcacac ggtggatttt gttgtctttg ctgcgggagt
     1621 aaaaccggaa accacactcg ccacgcagat gcagcttcaa attggaaaat cgggtggcgt
     1681 ggtcgtggat gaatttatgc aaacttcaaa ccccgatgtg tatgcagtcg gcgatatggt
     1741 tgaaataaaa aaccgcttta caggtctatc cacgcgcatc cctttggcag gccctgcaaa
     1801 ccgccaaggg cgtattgccg ctgaaaacat tgtatggggg aacaagagcc gttacgcggg
     1861 cgcgttaggt tcggcggtgg tcaaaatttt tgatacaaca ctcgcgttca ccggtcttac
     1921 ggaaaaggcg gcaaacgaat tgtctatcga ggcgggaacg gtgaccatcc atggtaacaa
     1981 ccatgcgggc tattacccca acgccgagcg catcaccctg aaacttattt ttgacacaaa
     2041 agacgggcgc gtgttgggag ccacagcgtt tggaaaggag ggcaccgaaa agcgcatcga
     2101 tgtgatcgct accgcacttg tgggaaagct tactgtgtat gatttagcca cgttggattt
     2161 atgctatgca ccgccgtatg gttctgcaaa cgatgtggtg aacgtggcgg cgttccaagc
     2221 gattaaccat ttgagtaaac tatcgcccgt ggtgacaccc gctgcgctcg atgcaaaccg
     2281 cgccgatttc aacgtggttg atgtgcgtat ttcaaaagaa agagacgaat gcaaaacgga
     2341 atgccaatgc gccatcgaag tggatacatt gcgcgagtgc ttgagcgatg tcccatgcga
     2401 caaaccggtt gcgctcttgt gtcaatcggg gcagcgcagc tatattgcat cgcgtatctt
     2461 aaaacaaaaa tgatttgata atgtgtataa tatcactggc ggttactacg cccttaaagc
     2521 ataccaagga gacaaaacat gactgaacaa caaattattt ggataagcgc attatcgctt
     2581 ttacttgtat ttattctata ccgtaaacat aaattgggga gaaacaaggt gaacgtaaaa
     2641 ggaatgttgc aacaaggagc aaaagtggtc gatgtgcgca gtgcgggtga atttgccgga
     2701 gggcattaca aaggggcgat caatattccc ctcgatgcgc tttcatcgcg cctcaataca
     2761 ttgggcgaca aatcaaaacc agtgattgtg tattgcgctt cagggatgcg ctcgggttca
     2821 gccaagcgcg ttttagagtc cgccggattt acgcaggtag aaaatggtgt gaaccagggt
     2881 tatttagagt cgctataatc tttctaacgt agacatgaat tttgatattt tacccaaata
     2941 tcaaaattca ttttttacgt gtcaatgtat tctcgtcggt gtggtcgaag ctgcggtcat
     3001 aaatcgaaag tgcggtgttc atcgtgtagc gcatggtgtc gccatcgacg ttgaccttta
     3061 gggtaaaacc gcgcgtcgat gctttggcgc tcataaacgg cgtttcgagc acgccgaagt
     3121 tagggtcttc ttttttcgct tcaacaaaga aatctacctg attgcctttc gtttgagcgt
     3181 tgccttttgc caatacagaa accgcacggg ggatggatag cgaataaaaa atttcattcg
     3241 tctttttttc ataataccaa taacccaact gatggtgaaa aacttcgccg ttgcttttcc
     3301 ggcacaccac ttgcttatac gaaagcacaa ccagcttttg ttcttctgca ttcaccgcgt
     3361 cacctaccgg ttcaatcgtg atggtctcaa aatagggcga atgctcttcg ccttcgggtt
     3421 cgggagaaat atccatgccc ttgtcgcccg tccacgtacc gatgagcggg aaaagcgggc
     3481 caaagaaaac tttaagtatg tcttgctctt ccatgcatgg catttaatta ttgaactggt
     3541 gcgtcaaccg taaagtagag aaatggggta aaaataaaaa aatgtcgaca agcgaatcga
     3601 attggtctat attcaaaaaa atcaatgaat aaaattcgca tcaccggcgc gcgcgaacac
     3661 aatcttaaaa acgtctcgct ggaaatccct cgtgaaaaac tcgtcgtcat caccggcctt
     3721 tcgggctcgg gtaaaagctc gctcgcgttc gatacgattt atgccgaagg gcagcggcgc
     3781 tacgttgaat cgctctcgtc gtatgcgcgg caatttttgg ggcagcttga aaaaccggat
     3841 gtcgattcga ttgagggcct gtctcccgcc atttcaattg aacaaaaaac taccgcacga
     3901 aatcctcgat caacggtggg cacggttaca gaaatttacg attacttgcg tttacttttt
     3961 gcacggttgg gcgatccgca ttgccccgag tgtggagcaa agctcgaagc gcaatcgatc
     4021 gacacgatgg tggcgtcggt ctttggtatt tttgatcgca tgccgaaatc gcagtcgata
     4081 cgcactcaaa ttttagcgcc gattgctcgt agtaaaaaag gcgagttcaa agatgttttc
     4141 gagcgcatcc aacgcgacgg ctttgtgcgc gtgcgtgtcg atggccagat taaaagttta
     4201 gatgaagaaa tctctcttaa aaaaaatttg aaacacgaca tcgatattgt ggtcgatcgg
     4261 ttggtgctcg aaaaagataa actccaagag caacgcatgc acgtcgcggg gtcgttagag
     4321 ttggcgctca agcaagcggt aactggcggt ggaagtggag gtcaagcgag cattcttttt
     4381 gaaggcgacg gcggcgaaca gctcgaagaa cacttttcgg cgaagttgta ttgtacctcg
     4441 tgcgagttga gttttcctga aattacacac cgtctttttt catttaactc acccgatggc
     4501 gcttgcgaag ggtgcagcgg tttggggcat acgcttgagt ttcaccccga caagcttatc
     4561 caaaacgatg aacaaacggt gcgagaaggt ataggcgaag ggttgggctt ttctgccgac
     4621 gcgtattggt ataaatcgtc gattgaggcg ctcgataaaa aattacactt tggggtagat
     4681 acaccgtggt gtaaattgtc caagaaagtg tgggaactcc tcctttatgg agataagtcg
     4741 ctgaaattaa attatgagtg gaaaggtacc gagagttcgt accagtattc gcgcggttac
     4801 gaaggattga taccgaattt gcaacgccga tatttgcaaa acgcttccga ctcgatgcgt
     4861 caaaaaatgg agcagtacat ggaagagatg ccgtgtgcgg tttgcaaagg caagcgcttg
     4921 aagccgattg cgctcgcagt taaactcaaa ggcaagaaca tcgccgagac aaccgcgctc
     4981 actctggaga cggcgctcga gtattttcaa aaaatacaat tcaacaaaac cgaacgcgaa
     5041 attgcgcagc aggcgatgaa agaaatttta gaccgtttac gctttttgaa ttcggtcggc
     5101 gtggggtatt taacgcttga ccgcatcgcc ggttcgcttt cgggtggaga agcgcagcgg
     5161 attcggttgg cgactcaaat cggctcggcg ctcatgggcg tgttgtatgt gttggatgaa
     5221 ccctcaatcg gtctacatca atcggacaat gaaaaactca tccaaacgct caaaggtttg
     5281 cgcgatttag gcaatagcat catcgtcgtt gaacacgnnn nnnnnnnaag agacgatgca
     5341 agaagccgac tttattgtcg acatggggcc gggtgcgggt aggcatggcg gcaacgttgt
     5401 cttcaccggc acatactcac aactcttaaa agccaaaaat tccttaaccg ccgattattt
     5461 aagcggtaag aaaaaaatcc ccgtccccac cgaaaggcgt aagaacgcta aagaatccat
     5521 ccgcattgtg ggagcgcaag aaaataatct taaaaacatc gatgttgagt tcccgttgtc
     5581 ggtgttcaca gtggtgaccg gactttcggg ttcgggcaaa agttcactcg tgaatgaaat
     5641 cctctacaag ggcatccaca aaaaactcat gcacacccaa gatttgccgg gcaaacacaa
     5701 ggcaatccat ggcgtggagc acgtggataa aattatcaac atcgaccaaa gtcccattgg
     5761 aagaacgccg cgctcgaacg ccgcaacgta cacgggcgcg ttcacgccca tccgtgattt
     5821 attcgccgcg ttacccgcaa gccagttgcg ggggtataaa cccggacgct tctcgttcaa
     5881 cgtcgaaggc ggtaggtgcg aggcgtgagc gggcgatggc gtcaagcgca tcgagatgca
     5941 ttttttgagc gacgtgtacg tgaagtgcga agtgtgccaa ggcaagcgtt ataatcaaga
     6001 gacgctcgaa gtgcgtttca aaaataaaaa tatttttgaa gttctcgata tggcagtcga
     6061 agacgcgctc gccttttttg aatcgatacc acaagtacac ccgaaactgc aagcgctcta
     6121 cgatgtcggc ttggggtaca ttaaacttgg ccaagcggcg acgacgcttt cggggggcga
     6181 ggcgcaacgc gtgaaactcg ccaccgaact tgcgcgccgc tccaccggca aaacgcttta
     6241 cattctcgac gaaccaacca ctggtctcca ctttgaagat attcgcttgc tcatgaatat
     6301 tttacaacgc tttgtggacg aaggcaacac cgttgtggtg attgagcaca atatggatgt
     6361 cgtcaagtgc gccgattaca tcatcgacat ggggccggag ggtggcgtca aaggaggtca
     6421 agtgattgcg acaggtacgc cggaagcggt cgcaaaaaat aaaaactcgc tcacggggaa
     6481 atttttacag cgttggttgg aatagaagag tacgacagat gggaacccgt gattctgtgt
     6541 gtgcggattc aaatctcttc ttttgaaatg agtattcgcc ctgtctttgc ttcgcgtact
     6601 gccccatggt gaatggcaaa cgtgaagatt tacttgttga aacattga
//
LOCUS       DAOMPD010000084         6640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4960_length_6640_cov_2.658770, whole genome shotgun sequence.
ACCESSION   DAOMPD010000084 DAOMPD010000000
VERSION     DAOMPD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4960_length_6640_cov_2.658770"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLY93_04200"
     CDS             <1..494
                     /locus_tag="PLY93_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68710.1"
                     /translation="PAGGAYYSYFGSSMLFTGSPGVSDGAGETNDTYIVRTPSSTTPY
                     ILTMGPHNLGAYAGLYADFTALSFIELSGAALISLIDIPLAGTSAMNSLYDKGTSLST
                     KAIQKWNDWKPFMIDLRATVHLSRLGIGFETPILVGYRAFGYPSLGLTEKGWYFGAVI
                     VND"
     gene            complement(498..1364)
                     /locus_tag="PLY93_04205"
     CDS             complement(498..1364)
                     /locus_tag="PLY93_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRP68711.1"
                     /translation="MKNRNIAIKLSGQNTTDGAGVRLKRFIGTQQISFLDPFLLLDEF
                     KNDDPAAYMAGFPNHPHRGFETVSYMIAGKMRHGDSRGNSGLLVPGSAQWMTAGRGII
                     HSEMPEMGTDGVFGENNTNKEMNPLLWGYQLWVNLPAAHKMTDPRYQDIPPEKIPEFE
                     TDDFVVRLVAGSYEGVQGAAETFIPVHYFDVIFKGEGVFEHAFPTTFNALLVGVHGSA
                     EVDGAAIREGDIVVFGEGDKVQIQGKEGTRVLFIAGEPIGETVARYGPFVMNTKEEIE
                     QAFVDYQAGVLA"
     gene            1441..2577
                     /locus_tag="PLY93_04210"
     CDS             1441..2577
                     /locus_tag="PLY93_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRP68712.1"
                     /translation="MEQELNRFVQEVKSRQAILCGKSHEDNRSRIPLKGTEVAAQIAK
                     TVYQKRKRGLIPSVAILPALLGLLSLSSGVGVMSRLSDTSLFEESIQKVSPSEGVVIW
                     EKQIRPALGRDFHLTLDLSNTLKAKTSQGKTIWRHALPPISATSSPVAFIKNSQIYIA
                     LSTANGVVYLLDALTGKALWTQDLDTPIDVTPVLIQNSIFAIACTDGRIYGLDAANGS
                     ITYMLQTDEKITAFKPVLDNKEQTIYATNDDKRVLSLNALTGDLLWNRETSGTASDSP
                     IITANKIITPTKDNDSAHLWAFENNGDLSWMTSFSPYSSFAANDNYLAVAEGTIVTLL
                     PADNGEPIYYWQLAAPPKDLALVGGHSGALFVKTDQGDLTATLN"
     gene            2614..3255
                     /gene="plsY"
                     /locus_tag="PLY93_04215"
     CDS             2614..3255
                     /gene="plsY"
                     /locus_tag="PLY93_04215"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRP68713.1"
                     /translation="MDFSLLVFVALFCYTIGSIPTAYLAARFIKGVDIRTIGSGNVGA
                     SNASRVLGRTWGILVMAFDALKGLVPVLLIQHLWFRNLPSAQTYAIVGAVFCLVGHIY
                     PVWLKFKGGKGVATGLGVMIALVPVAVAVAVPVFILVVFLTRMISVGSMSAALALPAA
                     FFIDHDLLNHRGLFIFTLGACLFVIYKHKSNIKRIIQGVENRLGDKNTHNQEE"
     gene            3258..3656
                     /locus_tag="PLY93_04220"
     CDS             3258..3656
                     /locus_tag="PLY93_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68714.1"
                     /translation="MKHLIGACLFFIFASGAIVAGPIRDEYFKTKDDIDKSKDTPEMR
                     KQFLEMNIQRCAVCWYVTTVIKNPIPSKFRTRITSSPKKINSHTILNLKTLLDFSPFR
                     ITPNTITLYHAKKKLYKNLTKHKTSKEAWH"
     gene            3647..4024
                     /gene="cutA"
                     /locus_tag="PLY93_04225"
     CDS             3647..4024
                     /gene="cutA"
                     /locus_tag="PLY93_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRP68715.1"
                     /translation="MALKSVSPLAIIGKKPHPLIILTALPSREIAEKVATALVEKQLA
                     ACVNIIPDVASVYYWDGEVVHDHECKLIIKTSSLVEMQVIDFIKTNHPYTVPEITVIG
                     HKSDVFMQPDYWSWLSTHVKHRR"
     gene            complement(4026..5141)
                     /locus_tag="PLY93_04230"
     CDS             complement(4026..5141)
                     /locus_tag="PLY93_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HRP68716.1"
                     /translation="MSNFKEEKKAMSKKAWWLYFPIVALVLLFAADKLLLLKKDKVTG
                     IVSGRGILLNETAKIRLDKEASFAAYRQRCRENCRTVVVFGSSRSERFNLLKQETRLS
                     EYLTAAQKKQIMDIQFLVYAQVGAQMAVYYHALDHLWKKNLIPDAIVLEIGPELLELE
                     HKNNNGKSEFLFSDEYDYDYYTFLAKYGKKSVRDEAIARLAFAGYALKPRPELLVYDY
                     LPKIQNEEPEYNIIERYHRSYKDYLESDLVGKLRDERIGQFVTMYSNNYKIFAVDPLM
                     DESLRRIVKMANEKNVPILLYRPFVHKELSDVLDKTPYAPAIHRYERELTGKHVKFYY
                     DSQDNYRCKYWSDASHFSTRCTPEVMRHLLGVLGIVR"
     gene            complement(5128..6510)
                     /locus_tag="PLY93_04235"
     CDS             complement(5128..6510)
                     /locus_tag="PLY93_04235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRP68717.1"
                     /translation="MNFISLIFLLFFIATFLIYWNIPKRARVGFLIFASLLYYGFYSP
                     AFLAHFVFVILLNYAFVYFTQKTKSRAWVALGVTLNITHLIFFKYFNALFTLLAPAVG
                     IATPAAFKVVMPLAISFYTFQFIAFQVDVYRGIQGNNPKYPQIDVSFSRYALFMLFFP
                     HLISGPILRSSDFYARIGDEQLNRESQVSGVYLIALGLFKKLFIADAIGYFINPIFKA
                     PLDYGSVDTLLVIFGYSAQLYTDFSGFIDMARGMAKLFGYDLPANFRSPYFSTSFSEF
                     WARWHITLSVFIREYLYIPLGGNRVGLFRQYFNLMTAMVLSGIWHGETLNFFIWGALH
                     GVYLCGEKILKISKNAETSLSWFIRNSWVVLGWSFSLIFFRSPDFQTAAQILKQVFVW
                     KTATLSWGFILLLIIVTWLIQFLEMRREKILAALVPRARIVIPALAVLLWFLISRVQI
                     PNEAFIYVQF"
BASE COUNT         1799 a         1612 c         1530 g         1699 t
ORIGIN      
        1 gccccgccgg cggcgcatac tattcttatt ttggttcgag tatgctattt accggttcgc
       61 ctggtgtgag cgacggtgct ggtgaaacca acgatactta cattgtgcgc actccatctt
      121 caacaactcc ttatattcta accatgggtc cgcataactt aggcgcgtac gccggtctct
      181 acgccgattt caccgcactg agttttattg aattgagcgg tgccgcgctt atatcgctca
      241 tcgatatacc gctcgccggt acgtcggcga tgaatagtct ttacgacaaa ggcacttcgc
      301 tttcaacgaa ggcaatccaa aaatggaacg actggaagcc cttcatgatt gacttacgcg
      361 ccaccgtaca cctttcgcgc ctcggcattg gctttgaaac gcccattctc gtgggctacc
      421 gcgcattcgg ctacccctct ttgggtttaa ccgaaaaagg gtggtacttt ggcgcggtga
      481 tcgtgaacga ctaacgatta agcaagcaca cctgcttggt aatccacaaa cgcctgctcg
      541 atttcttctt tcgtattcat cacaaaaggg ccatagcgcg ccacggtttc accaatcggc
      601 tcgcctgcaa taaaaagcac acgcgtgcct tctttgcctt gaatttgcac tttatcgccc
      661 tcgccaaaga caacgatgtc gccttcacgg attgctgcac cgtcgacctc tgcgctaccg
      721 tgtacgccga ccaagagcgc gttgaacgta gtggggaaag cgtgttcaaa gacaccctcg
      781 cccttgaaga taacgtcaaa gtaatgcacc ggtataaacg tctctgccgc gccttgaacg
      841 ccttcgtaac tgccggccac caaacgcacc acaaagtcat cggtttcaaa ttcgggtatt
      901 ttctcaggtg ggatgtcttg atatcggggg tcggtcattt tatgcgctgc cggcaaattc
      961 acccaaagtt ggtatcccca taaaagggga ttcatttctt tattggtgtt gttctcaccg
     1021 aacacgccgt cggtacccat ctcgggcatt tcactgtgga tgataccgcg accggcggtc
     1081 atccactgcg cagaaccggg aaccaagagc cccgaattac cgcgtgaatc gccatgccgc
     1141 attttgcctg caatcatata tgacacggtt tcaaagccac ggtgtgggtg gttgggaaat
     1201 cccgccatat acgcggcggg atcgtcgttc ttgaattcat cgaggagcaa aaagggatcc
     1261 aaaaacgaaa tttgctgcgt accgataaaa cgcttcaggc gtacacccgc accatcggtg
     1321 gtattttgac cagaaagttt tatagcgata ttcctgtttt tcatactcgt attatactat
     1381 aatttttaga atatttgtat ttttttatgg acacttacgt atgtttatgt aagataaagt
     1441 atggagcaag aactcaaccg cttcgtccaa gaggtaaagt ctcgccaagc cattttatgt
     1501 ggaaaaagcc acgaagacaa ccgaagtaga ataccactaa aaggcacaga ggtagctgct
     1561 caaattgcta aaacagtgta tcaaaagcgg aaacgggggt taatccccag tgttgcgatt
     1621 ctccctgctc tcttagggtt gctttctctc tccagcggcg tgggtgtcat gtcgcgtctc
     1681 tccgacacat cgctctttga agagtccatc caaaaagtga gtccttccga aggcgtcgtt
     1741 atatgggaaa agcaaatccg tcccgcatta gggcgcgatt ttcacctgac tcttgattta
     1801 tccaatactc tcaaagcgaa aaccagccaa ggcaaaacaa tatggcggca tgcactcccc
     1861 cccatttcag ccacttcatc acccgttgcg ttcattaaaa actctcagat ttatatcgca
     1921 ctttccacgg caaacggtgt ggtgtattta ctcgatgcac tcaccggtaa agcgttgtgg
     1981 acgcaagatt tggacactcc catcgacgtg actcctgtgc tcatccaaaa ttctattttt
     2041 gctatcgcct gtaccgacgg ccgcatttac ggcttggacg ccgctaatgg tagcatcact
     2101 tatatgctac aaaccgacga aaaaattacc gcctttaaac cggttttaga taataaagaa
     2161 cagacaattt atgccaccaa cgacgataaa cgcgtcctca gcctcaacgc tttaaccggc
     2221 gatctcctat ggaaccgcga gacatcgggc acagcgagcg acagccccat aatcaccgcg
     2281 aacaaaatta tcactcccac caaagacaac gacagcgctc atttgtgggc ttttgaaaac
     2341 aacggcgatt taagttggat gaccagcttc tcgccctact cgtcgtttgc agccaacgac
     2401 aactatctcg ctgtcgccga ggggactatc gtcacgttgt tgcccgccga caacggcgaa
     2461 cccatttatt attggcagct cgccgcgccc cctaaagatt tagcgctcgt cggcggtcac
     2521 tcgggcgctc ttttcgtgaa aaccgaccaa ggcgatctca ccgccacact caactaatcg
     2581 ttgacagagc gcatcccgcc cctatttttt gatatggatt tctctttact tgtttttgtt
     2641 gccctttttt gttacacgat aggttcgata ccgactgcat acctcgccgc gcgctttatt
     2701 aaaggcgtgg acatccgcac gattggatcg gggaatgtcg gtgcgtcgaa tgcgtctcgc
     2761 gtactcggcc gcacgtgggg tattttagtg atggcattcg atgcactcaa agggcttgtg
     2821 ccggtgctcc tcattcaaca cctgtggttt cgtaatttac cttccgcaca aacctacgcc
     2881 atcgtcggcg cggttttttg tctcgttggg catatttatc cggtgtggct taaattcaag
     2941 ggtggcaaag gggtcgcaac cggattgggt gtgatgattg cactcgtacc cgttgcagtt
     3001 gcagtggcgg tgccggtttt tattctggtc gtctttttaa cgcggatgat ttctgtaggt
     3061 tcaatgagtg cagcgctcgc gctaccggcg gcatttttta tcgatcacga cctactcaac
     3121 caccgtggcc tttttatttt taccctcggc gcatgtcttt ttgtgattta caagcataag
     3181 tctaatatca agcgtatcat tcaaggggtc gaaaatcgac tcggcgacaa aaacacacat
     3241 aaccaagagg agtaactatg aaacatttaa tcggggcctg cctatttttt atttttgcca
     3301 gcggcgcaat cgtggcaggc ccaatccgcg acgagtactt caaaaccaaa gacgacatcg
     3361 ataaaagtaa agacactcca gagatgcgta aacaatttct tgaaatgaat atccaacgtt
     3421 gcgccgtttg ttggtacgtt actacggtta tcaaaaaccc gattccatca aaatttcgaa
     3481 ctcgaattac gagcagtccg aaaaagataa actcacatac tattttaaat ttgaagactt
     3541 tgttggattt ctcacctttt cgaataaccc cgaatactat tacgctttac cacgcgaaga
     3601 aaaagttata caaaaaccta accaagcaca aaacaagtaa agaggcgtgg cattgaaatc
     3661 tgtcagtccc ctcgccatca tcggtaaaaa accgcatccg ctgattatac tcacggcgct
     3721 acctagccgt gagattgcgg aaaaagttgc caccgctctt gtcgaaaagc aactcgcggc
     3781 atgtgtgaac attatccccg atgtagcatc ggtatattac tgggacggtg aggttgtgca
     3841 cgaccacgaa tgcaaactca ttattaaaac atccagcttg gtcgaaatgc aagtgattga
     3901 ttttattaaa acaaaccacc cctataccgt gccagagatc acggtgattg gccataaaag
     3961 cgatgtgttt atgcagcccg actactggtc ttggttgagt acgcacgtca aacaccgacg
     4021 ttaattcatc gaacaatccc caacacaccg agtaagtggc gcatcacctc gggggtgcag
     4081 cgtgtggaaa aatggctcgc atccgaccaa tacttacagc ggtagttatc ttgtgaatcg
     4141 tagtaaaact taacgtgttt tccggtgagc tcgcgctcgt agcggtggat tgccggagcg
     4201 tacggtgttt tgtcgagcac gtcagaaagc tctttatgca caaaaggtcg atagaggagt
     4261 atcggcacgt ttttctcatt cgccattttc acaatgcggc ggagcgattc gtccatcaaa
     4321 ggatcaaccg caaaaatttt atagttattc gagtacatcg tcacgaactg gccgatgcgc
     4381 tcgtcgcgca gttttcccac aaggtcactc tctaaataat ctttatagct gcggtggtag
     4441 cgttcgatga tattatattc cggttcttcg ttttgaattt tagggagata gtcgtacaca
     4501 aggagctcgg ggcgtggctt tagtgcatag ccagcgaaag caaggcgggc gatggcttcg
     4561 tcgcgcaccg atttttttcc atacttggct aaaaacgtat agtaatcata gtcgtattcg
     4621 tcgctaaaga ggaactccga cttaccgtta ttgtttttgt gctctaactc taaaagctcg
     4681 ggaccgattt ccagtacaat cgcgtcgggg attaaatttt ttttccagag atggtcgagc
     4741 gcgtggtagt acaccgccat ttgtgcgccc acctgtgcat acactaaaaa ctgtatatcc
     4801 attatttgct ttttttgggc ggcggtgagg tattcagaaa ggcgggtttc ttgtttcaat
     4861 aaattgaatc tttcggaacg cgagctgccg aagacaacga cggtgcggca attctcgcga
     4921 caacgttgac ggtatgcggc aaagctggct tctttatcca agcggatttt agctgtttcg
     4981 ttgagtaaaa ttcccctccc cgatacaata ccggttactt tatccttttt taggagcaat
     5041 aatttatcgg cggcaaaaag caagacgaga gcgacaatgg ggaaataaag ccaccaggcc
     5101 tttttagaca tcgccttttt ttcttcctta aaattggaca tagataaacg cctcgttggg
     5161 aatttgtacg cgtgaaatta aaaaccacaa aaggactgcc aatgcgggga taactatcct
     5221 cgcgcggggt acaagtgcgg ctaaaatttt ttcacggcgc atctctaaaa attggatgag
     5281 ccaggtgact ataatgagta gaagtatgaa tccccacgaa agggtggccg ttttccatac
     5341 aaagacctgt ttgagtattt gggcagccgt ttgaaaatcg ggcgagcgga aaaaaattaa
     5401 agagaagctc caccccaaaa caacccaact gttgcgtata aaccagctca aagaagtttc
     5461 ggcgttcttg gatattttta atattttttc cccacataaa tacacaccgt ggagcgcgcc
     5521 ccaaataaag aagttgagcg tctcgccgtg ccaaatccct gaaagcacca tcgccgtcat
     5581 gaggttgaag tattggcgaa aaagacctac acggttgccc cccaacggga tatataaata
     5641 ctcgcggata aacaccgaga gggtgatgtg ccaacgcgcc caaaactcag aaaagcttgt
     5701 tgaaaaataa ggactgcgga agtttgcggg taaatcgtac ccaaaaagtt ttgccatgcc
     5761 gcgcgccatg tcgataaaac ccgaaaaatc ggtgtagagc tgtgcactat acccaaaaat
     5821 aacgaggaga gtgtcgacgc tgccataatc caacggcgcc ttaaatatcg ggttgatgaa
     5881 ataaccgatt gcgtcggcga taaaaagttt tttgaaaagc cccaacgcaa tgaggtacac
     5941 gccgctcact tggctttcgc ggtttaattg ttcgtcgccg atgcgtgcgt aaaaatctga
     6001 cgaacgcaaa attggtcccg aaataaggtg tgggaaaaaa agcataaaaa gagcgtaacg
     6061 agaaaacgag acgtctatct gtggatattt gggattattc ccttggatgc cacggtacac
     6121 atcgacttga aaagcgataa attggaacgt gtaaaaacta atcgcaagcg gcatcaccac
     6181 tttgaaggcc gcaggcgtcg ctatacccac cgcaggcgca aggagtgtaa agagagcgtt
     6241 aaagtatttg aaaaaaatta agtgggtgat gttgagagtg actcccaacg ctacccacgc
     6301 acggctttta gttttttggg tgaaatatac aaaggcataa ttgagcaaaa tgacgaaaac
     6361 aaaatgcgct aaaaatgcag ggctataaaa cccatagtac aagagagaag cgaaaataag
     6421 aaatcccacc cgtgcgcgtt tggggatgtt ccaatagata aggaaagtgg cgatgaaaaa
     6481 gagtaaaaag atgagcgaga tgaaattcat agattatgga gtaacttgca taaaattgta
     6541 tattcggtca acctttctaa gcaaataaga gtcgatggta tttttactta ccctcggcgg
     6601 ggaggtcgga caatctttgg ccaaaccata gtttttgaag
//
LOCUS       DAOMPD010000085         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_4984_length_6601_cov_2.815307, whole genome shotgun sequence.
ACCESSION   DAOMPD010000085 DAOMPD010000000
VERSION     DAOMPD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4984_length_6601_cov_2.815307"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLY93_04240"
     CDS             <1..654
                     /locus_tag="PLY93_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP68718.1"
                     /translation="PEGSGKSQIMSLIMARQVPHAGSIYFQGVPLEKKSDEAMEMLRF
                     SIGYVAENLGLINNLSVLENILLPLRYHTALKDDELYAAADLWLERYDLKHKEKIRPV
                     SLSASESMRTALLRALIVEPRILLLDGIFDGGCPLASRYLMDLLFEDIKLRGISFIIT
                     TYFPFFFDGRDLQFLLLYRGRPVFQGALADMRTTDNEYAAQYRTLNAIGPMRSFNET"
     gene            654..1367
                     /locus_tag="PLY93_04245"
     CDS             654..1367
                     /locus_tag="PLY93_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRP68719.1"
                     /translation="MKFDRNDIFTGLFMVVGAVFALTLILFIAGYSLLDDRTEYWIRF
                     EKLAGVKKGTQIRIKNFTIGEVTEVIPIYGSDLTFKARARINKEFVIYQGSGLTITNQ
                     NVIGDAVIDLTPSLVKKYVLHPHDTLFATNFVNLEQMVDQISNLVATINKLVQTFTSL
                     TGDNKDDIKMILGNLNGSIAKVNGLLGASQGEILAIMRNIRSTSLTLDRVAKKMERDP
                     MGFLGSKGNNSTSASAALP"
     gene            1404..2933
                     /locus_tag="PLY93_04250"
     CDS             1404..2933
                     /locus_tag="PLY93_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP68720.1"
                     /translation="MRRIILFSTLAASALWAEITVTPLGNGINSRYDELAPVLSPDGN
                     TIYFCREGHPDNIGVATIVNGMSALREGQDIWVSHKRPDGTWSEAEHLKAAFNSRTYD
                     FPIGTSADGKTLYIGNVYNRDGSVRPGVSKTYLRNGKWSFPESLRIENFYNEANLVNY
                     SLSADERTLILNVQRKDTVGKMDLYVSFLQADDSWSEPLNLGREINSPFMEVTPFLAA
                     DNKTLYFASNRPGGVGQFDMYMSRRLDDTWTHRSEPINLGPKINTVGNDINYVIASTG
                     DYAIFASDTPGKGKDLYRISLPEELRPEKSIVVYGRVLNNNGEGVESRVVFERLSDGA
                     LLARTETGENGEFRMHLPMGEVYGIHAESGGYLPQSANINLKDGFTKNTPLELTMYPL
                     KAGSKMPINNLFFQKGSYKLSKASYAELNRLTKLLRARPNLKIEIGGHTDNTGNPKSN
                     MTLSKNRARAVMDYLIEKGFPRKNIKAVGYGQEKPVASNATGEGKKKNRRVELTILSA
                     D"
     gene            complement(2985..3143)
                     /gene="rpmG"
                     /locus_tag="PLY93_04255"
     CDS             complement(2985..3143)
                     /gene="rpmG"
                     /locus_tag="PLY93_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRP68721.1"
                     /translation="MREIVKLESTAGTGFFYTTTRNKKAQGGKLEVMKYDPKVRKHVK
                     FIEKKASK"
     gene            3228..3683
                     /locus_tag="PLY93_04260"
     CDS             3228..3683
                     /locus_tag="PLY93_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68722.1"
                     /translation="MPMIVIRKIELYAAALAVVALVLTSLTIILLRMFSTSAWSVGVV
                     DTLSQYPSHFMVVAALLGGSIAMSRGETLKIEVLNSVMGKQAQVMTRRIVALLGFVFF
                     GVFIALAVRYLTIDYRPVVAYVYLPLMVLLWLKILAEVFVKTHKPLHEI"
     gene            3685..>4125
                     /locus_tag="PLY93_04265"
     CDS             3685..>4125
                     /locus_tag="PLY93_04265"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRP68723.1"
                     /translation="MYSMPRASWIVLRLVWIQVALCAAVLVESAIFHRSQIADLFVLN
                     YYSIFPLGEVWRLLTAVLVHTNPFQLLFILLTLWMFGSELEQRWGRSVFLRFYLLCAL
                     SSSLTFLFLGAFFPMMRSDIFMTSTGPVLGLLIAYAIYWPERQVW"
     assembly_gap    4126..4135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4136..4553
                     /locus_tag="PLY93_04270"
     CDS             <4136..4553
                     /locus_tag="PLY93_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68724.1"
                     /translation="SFSKPTWEVHFFFLFPIRMKYVALITGVIAILYAVFSAPFVLSA
                     AGHVGGLLTAGLILLSTGGEPSRLNRPIEHLFKSWRRVKQTSPKENAFTGSGENLETQ
                     IDVILDKISRGGMKSLSNAEKQFLRAASERMNRSKN"
     gene            complement(4582..6258)
                     /locus_tag="PLY93_04275"
     CDS             complement(4582..6258)
                     /locus_tag="PLY93_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68725.1"
                     /translation="MISGVIFSLFIGRFLENPQWAISFAFVAIVFARLQKQRTYNVTH
                     ERLVPLRQFIDLLRYLFLAQAFHSILDINREQLLFVVLICILGFIVPQLIRRFGTLRP
                     HIQQGLLFLPIIFSVLAILFNLISYSYVGAIAYLLLATWEALYFKKAHEIYLKREKVL
                     AGIAILAAISAYYIASEWMQILLGALIALLLGGILFYVAKNWRKTITALFAAATLLWI
                     YAIQVKYTNSVTRNFFRPPVLRIHSPQLPNVALMATIFSQREKKAERLYTNILPQELF
                     NDNAWQGQNIERFEVNPAFLTFRLAYSCYLRKQNRTYILDEKSLEHYADPSALVALGK
                     MFTFFGRCELYTADGKTLRNINNLGQVLSSDTAEILKSISKETAPLLLSLARAEKRHD
                     LTNEAYALYEQIFSYYKDDPIFLRELSSLAAARGLIDRQIELLNSLVRIAKDDTLYDK
                     KMLMELYALKGERKKSAALAYEILASNSGESSLAIYAFLQKLFSDPFDRYEMEALYRK
                     ISAYKPKTDLEELKLNGLKRIVEEQVKQNPTSDLKFRNENHRQEFITFPE"
     gene            6367..>6601
                     /locus_tag="PLY93_04280"
     CDS             6367..>6601
                     /locus_tag="PLY93_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68726.1"
                     /translation="MKKIKKEKDESPRKKSNAGRDREIAPEFRVRNFVECGGYKSNGE
                     YNERKESQREKSARVGEEVIEQVAKKKKKSKSPQ"
BASE COUNT         1782 a         1389 c         1599 g         1821 t
ORIGIN      
        1 cccgaaggga gcggcaagag ccaaatcatg tcgctcatta tggcgcggca agtgccacac
       61 gcagggagca tttattttca gggtgtgccg ttagagaaaa aatccgacga agcgatggag
      121 atgctccgtt tttcgattgg ttatgtcgct gagaatttag gacttatcaa taatttgagc
      181 gttctggaaa atattttatt acccttacgc taccacactg cactcaagga cgatgaatta
      241 tacgcagcgg cagatttatg gctggagcgc tacgacttaa aacataaaga aaaaatacgc
      301 ccggtttcgc tttccgcgtc tgaatcgatg cgcaccgcgc ttttgcgcgc gctcattgtc
      361 gaaccgcgca ttcttttact cgacggtatt tttgacgggg ggtgcccctt ggcgtcgcgt
      421 tacttgatgg accttctatt tgaagacatt aaactccgtg gtattagctt tattattaca
      481 acgtactttc ctttcttttt tgatggacgc gacttgcagt ttttactcct ctaccgtggt
      541 cgccctgttt ttcaaggggc attagccgat atgagaacga ccgataacga atatgcagcg
      601 cagtaccgca ccctaaatgc aataggaccc atgcgttctt ttaatgaaac ctaatgaaat
      661 tcgaccgtaa cgacatcttt accgggctat ttatggtggt gggggcggtt tttgcgctga
      721 cgcttatttt atttattgcg ggctatagcc ttttagacga cagaaccgaa tactggattc
      781 gttttgaaaa actcgcggga gtgaaaaaag gcacgcaaat ccgtatcaaa aattttacca
      841 tcggggaagt caccgaagta atccctattt acggtagcga tttgacgttc aaggcgcgtg
      901 cgcggatcaa taaagaattt gtcatctatc aaggttcggg gctgacaatc accaaccaga
      961 atgtcattgg cgatgcagtc atcgatttaa cgccgtcgtt ggtgaaaaaa tacgtgttgc
     1021 atccgcacga cacacttttt gccacaaact ttgtcaattt agaacaaatg gtcgatcaaa
     1081 tctctaactt ggtggcaacg atcaacaaat tagtgcaaac tttcacttcg cttacgggcg
     1141 acaacaaaga cgacattaaa atgattttag gcaatctcaa cggttctatt gcaaaagtca
     1201 acggtctttt aggcgcatcg cagggtgaaa ttttagcgat tatgcgcaac atccgcagca
     1261 cgtcgttgac gcttgaccga gttgctaaaa aaatggagcg cgacccgatg ggctttttag
     1321 gttcaaaggg aaacaatagc acaagcgcgt cggcggcatt accctgaact tacgaatacc
     1381 gttgagcgtg ccgatataaa tatatgcgac gaatcatcct atttagcact cttgccgcct
     1441 ctgctctttg ggcggagatt accgttactc cgttggggaa tggaatcaat tcgcgctacg
     1501 acgaactcgc gccggtttta tcgcccgacg gcaacacaat ttatttttgc cgcgaaggcc
     1561 atcccgacaa cattggcgtg gcgacgattg tcaacggtat gagtgctttg cgcgagggcc
     1621 aagatatttg ggtgagccac aaaagacccg acggcacttg gagcgaagcg gaacacttga
     1681 aagcggcgtt caattcacgc acgtacgatt tcccaatcgg tacatccgcc gacggcaaga
     1741 cgctgtatat aggcaatgtc tataatagag acggttcggt gcgcccgggc gtatcaaaaa
     1801 catatttgcg caacggcaaa tggagtttcc ccgaatcgct gcggattgaa aatttttaca
     1861 acgaagcgaa tttggtaaac tattcgcttt ctgccgacga gcgcacgctc atcttaaacg
     1921 tgcagcgcaa agacaccgtc ggtaaaatgg atttatacgt gagcttttta caagccgatg
     1981 attcatggtc ggaacccttg aatttaggac gtgaaatcaa ttcacctttt atggaagtca
     2041 ctcccttttt ggctgccgac aacaagactc tttattttgc gtcaaaccgg ccaggtggag
     2101 tggggcaatt tgatatgtac atgtcgcgtc gcttagacga cacctggacg cataggagcg
     2161 aacccattaa cttaggtcca aaaattaata cggtggggaa cgacatcaac tatgtgatcg
     2221 cctcaacagg ggattatgcc atttttgcct ccgatacccc cggcaaagga aaagacctct
     2281 atcgaatttc actccccgaa gaattacgcc ccgaaaaatc tatcgtggtg tacggtcgcg
     2341 ttttaaacaa caacggcgaa ggggtcgaat cgcgtgtggt cttcgaacgc ctaagcgacg
     2401 gcgcactttt ggcgcgcact gaaaccggcg agaacgggga atttagaatg catttaccca
     2461 tgggcgaagt atatggtatt cacgccgaat cgggcggtta tttaccgcaa agcgcaaaca
     2521 tcaacttaaa agacggattc accaagaaca cgccgcttga gctcaccatg tacccgctta
     2581 aagcgggctc gaaaatgccg atcaataatc ttttctttca aaaaggatcg tataaacttt
     2641 ctaaagcatc gtatgcagag ctcaaccggt taacgaaact cttgcgcgct cgtcccaatc
     2701 ttaaaattga aattggcggg cataccgaca acacgggtaa tcctaagagt aatatgacgc
     2761 tttcaaaaaa ccgagcgcgc gcggtgatgg attatttaat cgaaaaaggt tttccccgta
     2821 aaaatattaa agccgtggga tatggtcaag aaaaacctgt ggcatcgaat gcaacaggag
     2881 aaggcaagaa aaagaaccgc cgcgtagaac taactatttt atctgcagat tagaatctat
     2941 ttattttcct ctttgtgcat acgtacaaag aggaaaataa agtattattt agatgctttc
     3001 ttttcgatga attttacgtg tttacgaact ttaggatcgt atttcatcac ttcgagttta
     3061 ccgccctgtg cttttttgtt acgggtggtg gtgtagaaaa aaccggtacc ggctgtcgac
     3121 tcgagtttaa caatttcgcg catggaagct gaaatagcgg tggcgatacg gtgtaaatct
     3181 tatttacggt tggcgcacag actctatctt agacatttgt ttatagcttg cccatgattg
     3241 tcatccgtaa aatagaacta tacgctgccg cgctggcggt ggttgcatta gtactcacct
     3301 cgctcaccat tattcttttg cgcatgtttt cgacgagtgc atggtcggtt ggggtggttg
     3361 atacgctttc gcaatacccg tcgcacttta tggtggttgc tgcgctttta ggcggttcga
     3421 ttgcgatgag ccgaggcgaa acgctcaaaa ttgaagtctt aaacagcgtt atgggcaagc
     3481 aggcgcaagt aatgactcgg cgcatcgtcg ctttgttggg ttttgttttc tttggagttt
     3541 ttatcgctct ggcggttcgg tatttaacga ttgactatag acccgttgtc gcctatgtat
     3601 atttaccgct catggttctt ttgtggctta aaattttagc tgaagttttt gtgaaaacgc
     3661 ataaacctct tcacgagatt taatatgtat tcaatgccgc gcgccagttg gattgtttta
     3721 cgtctcgtgt ggatacaagt ggctttgtgt gcagccgtgc ttgtggagag cgccattttc
     3781 catcgctcac aaattgccga tttatttgtt ctcaactatt attcaatttt tccgttgggt
     3841 gaggtgtggc gacttttgac cgcagtgttg gtgcatacca atccgtttca actcttattt
     3901 atcctactca cactgtggat gttcggttca gagttggagc aacgctgggg gcgcagcgtt
     3961 tttttgcgtt tttatctttt gtgcgccttg tcgtcgagtt taacgttcct ttttttgggg
     4021 gcattttttc cgatgatgcg ctcggatatt tttatgacat cgactggacc ggtgttgggg
     4081 cttttaattg cgtatgcaat ttattggccg gagagacaag tgtggnnnnn nnnnnctctt
     4141 tttcaaagcc tacatgggaa gttcatttct tttttctttt ccctatccgc atgaagtatg
     4201 tcgcgcttat taccggcgtc attgcaattc tatacgccgt tttttcagcc ccttttgtgt
     4261 tgtcggcggc aggacatgtg ggtggtttat tgacagcagg gctcattctt ctttctaccg
     4321 gcggtgaacc gtcgcgcctc aatcgcccca ttgagcattt gtttaaatcg tggcggcgcg
     4381 tgaaacaaac gtctcccaaa gaaaatgcat tcacggggag cggtgaaaat ttagaaacgc
     4441 aaatcgacgt tattttagac aaaatttcac gcggcggtat gaaaagttta agtaacgccg
     4501 aaaaacaatt tttacgtgcg gcgtccgagc ggatgaaccg ttctaaaaat taatcatact
     4561 tctaaatcag atctctaatt tttattcagg aaatgtgata aattcttgtc tatggttttc
     4621 atttctgaat tttagatcgc ttgtggggtt ttgtttcact tgttcttcga cgatacgctt
     4681 taagccgttt agtttgagct cttctaaatc ggttttgggt ttgtacgccg atattttacg
     4741 gtagagcgct tccatttcgt agcgatcgaa cggatcggaa aaaagttttt gtaagaaagc
     4801 ataaattgcc agtgaggatt cgccgctatt gctagcgaga atttcatagg cgagggcggc
     4861 actcttttta cgttcgcctt taagtgcata aagttccatc aacatttttt tgtcgtagag
     4921 agtgtcgtct ttggcgatgc gcacaaggct gttcagtaat tcgatttggc ggtcgatcag
     4981 cccgcgtgct gcggcaagcg atgaaagctc gcgcaagaat atgggatcat ctttgtagta
     5041 tgaaaaaatt tgctcgtaga gcgcgtacgc ttcgttggtt aaatcgtgcc gcttctcggc
     5101 gcgcgcgagg cttaaaagaa ggggggcagt ttctttagat atactcttga ggatttcagc
     5161 cgtatcgctc gacaaaactt gccccaagtt gtttatgttg cgcagtgtct ttccgtcggc
     5221 tgtgtaaagt tcgcagcggc caaaaaaggt gaacattttt cctaacgcca ccaaagcgct
     5281 ggggtcggcg taatgttcta aacttttttc atcgagaata tacgtacggt tttgtttgcg
     5341 taaataacac gagtacgcca agcgaaacgt caaaaaggcg ggattgactt caaagcgttc
     5401 gatattttgt ccctgccaag cgttgtcgtt aaaaagttct tgcggtaaaa tgttggtgta
     5461 gagccgctct gccttttttt cccgttgtga aaaaatggtc gccataagcg ccacgttggg
     5521 tagctgcggt gaatggatgc gcaacacggg gggacggaaa aaattacgcg tgaccgagtt
     5581 tgtatatttt acttgtattg cgtatatcca aagtagcgtt gcagcggcaa aaagcgctgt
     5641 gattgttttg cgccaatttt tagcgacgta aaaaagaatg ccgcccaata aaagcgcaat
     5701 gagcgctccc aaaagaattt gcatccattc cgaggcgata taatatgcac tgattgcggc
     5761 aagaatggca atgcccgcca gtactttctc gcgctttaaa tagatttcat gcgctttctt
     5821 aaaatagaga gcttcccatg tcgcaagaag taaataggcg atcgcaccaa cgtacgaata
     5881 agaaattaag ttgaagagaa ttgctagtac gctgaaaata ataggaagga aaagaagtcc
     5941 ttgttgtatg tgcggtctaa gcgtgccaaa ccgacggata agttgcggca cgataaagcc
     6001 taaaatacaa atgagaacga cgaaaagtaa ttgttcgcgg ttaatgtcta aaatggagtg
     6061 gaatgcttgc gctagaaata aatagcgtag caagtcgata aattggcgta gtggcaccaa
     6121 gcgttcgtgc gtgacgttgt aagtgcgttg tttctggagg cgcgcgaaaa cgatggcgac
     6181 gaatgcaaaa ctaatcgccc attgaggatt ttctaaaaac cgtccaatga aaagagaaaa
     6241 gataacgcct gaaatcaaat ataatttaac cgtatgaaaa aagaatgcgt gcctggataa
     6301 ttcataccaa agataaagcg agggtagaaa cagtatgatg ggaaaataaa gaaaagcata
     6361 tttagcttga aaaaaattaa aaaggaaaag gatgaatccc cccgaaaaaa aagtaatgca
     6421 ggacgagatc gcgaaattgc gccagagttt cgtgtcagaa actttgtaga gtgcggcggc
     6481 tataaaagca acggcgaata caatgagcgc aaagagagtc agcgggaaaa aagcgcgcgc
     6541 gtaggggaag aagttatcga gcaggtagcg aaaaaaaaga aaaaaagtaa atccccacaa
     6601 g
//
LOCUS       DAOMPD010000086         6580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5004_length_6580_cov_2.199234, whole genome shotgun sequence.
ACCESSION   DAOMPD010000086 DAOMPD010000000
VERSION     DAOMPD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5004_length_6580_cov_2.199234"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLY93_04285"
     CDS             complement(<1..485)
                     /locus_tag="PLY93_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68727.1"
                     /translation="MAVVEARSSLYRRAQQLTENDPREAEALYVKFIRETGAHKLKRA
                     AYHELFALRLKEARLAEAFFQINKKSFRRRYVSAMEENLHLSNRRAQRLLSVLERECN
                     PERDTEKLREYLEASKATVLEYNYVLRVQTLCGNNDTLAVLPELPVSNDDFDENKFYT
                     AL"
     gene            complement(524..3121)
                     /locus_tag="PLY93_04290"
     CDS             complement(524..3121)
                     /locus_tag="PLY93_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRP68728.1"
                     /translation="MLHAFEHACRRCARPFSPQLNLTSYSILTADCPHCGMSHGYDNR
                     EGKLTKLLTAKVASLRNEQRGIPQTHRVHTLPPVTQTSTQNFQAPAPPPPLEQNASLY
                     ENSLSSQTEQREVYTKTKLPTWPQLKEKLLTLKENLTFSKPNNAWAKLLFSGVAFLFI
                     LGFTLFIWQAIPASNDEAKEAVRLLKARQTDVVLDRSGHELTKLGVSQSDKITLSAFQ
                     PWQIQALLYSEDRGFYKHHGVEYTAIARAFLNNLLRLRYAQGASTITQQLARIMLQNR
                     EKSIFRKVREARLARALENVLTKKEILELYVNNVYLGHGTYSFASAAKFYFSKPVKDL
                     SPNEFLAIVALIPSPERLSPIKNNARLQARMNALFEGMRSTGILSLSVHQWNQAMVSV
                     SEQKERFASETAFGEKSRLGLWPAEYAREFLSQRNILSSESQGSAKVYTTIDAAMQRR
                     AEELLHTHLVSARKRFHGRIRSSDGASVRLKNSVRHAAFDAGLLLDLGGFTPVTNAEP
                     LLQAALIAIKPTTGEILAMVGGDGFESNNQLNRTVKMRRQTGSAIKPFIYAKALSTHQ
                     INPATLIDDSPYIAGSGSRTWAPENINGDFEGPITARTALAKSRNIPAIRVGRLLGRD
                     AVTELFYDFFFQDETQQSERFAYDETVAIGTISLSPLEMARAFSVFANQGYLNNPVLL
                     TRLETPDRVIDLKNTHAHQLGLKQAPKERLLSAAETELMISLLKSSGSFSGTSLAGVI
                     GKTGTTSNSRDLWFVGGGKDVIVAVWFGYDDMRYSIPGATGSALASKLAADFLRKDFA
                     PIPFKMKPGMVRLRVCPLSGKLASSTCSHAQSEVFISGVVPQGECLHGKAVDLENDFV
                     AIMGDSQFH"
     gene            complement(3126..3854)
                     /locus_tag="PLY93_04295"
     CDS             complement(3126..3854)
                     /locus_tag="PLY93_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68729.1"
                     /translation="MFKKKLWLISFFAVAGLLGVLWAQKIIDPQILAQQAEEKRRAEE
                     TGYGDIEIDNLHREIAQLALKAAPRMQKEADGGLSLEIASGESIKTISLRYLYDGRIV
                     LYPSGDEKAAKLSKVKLVFERTNPFGKEYKREKREVINNTPLFFDNDESKDPSQKNET
                     KVDRNDDMVVIYYESEIDPRGQESFKEKEKVDLKTIPFYDKKMDFIETYKKYLRRTVK
                     SLRYTVESIEITQKKHLRDLFELK"
     gene            complement(3911..>5407)
                     /locus_tag="PLY93_04300"
     CDS             complement(3911..>5407)
                     /locus_tag="PLY93_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRP68730.1"
                     /translation="VAARYGMENVLAVMSDFFQEANERNKFGDTALIESARSGNIKAV
                     ELILKYKPSVPAANYYGHTAVSQARFDNFTDIARLLESFGAENPAASKELLQATQEKN
                     LMKAMGAVNRGANVNVVDEKGYSPLSYAVELADKLMIEYLRSKGALNALASRRLVLAI
                     RTANGDVFRKSLNEYADVNYTDKDLQTPLLIAAAFGNYEMLKELLERGAQVNARDKDG
                     YSAVFYAAQENRVRIFDELIKYKADIKRKNNFGYTPLILASVEMVRRLVNAGVDVNAT
                     ADNGGTAAYWAAIKDYDEVVKILVGAGAVDPKNNKELVMAARSNDVARAERALESNIN
                     IDLKNEEGYTALMYAVNSGANDVAKFLLDRKARVNLQDRDGYTPLMIALFRGDASMVD
                     MLMQYRPDVNLRHRSGRTALIMATWRGNTEMMALVLKAGARVNDQDLDGWTALMFAAH
                     EGNMAKVDLLIKSRALAKIKNKKGLMAEDLAHARHAEDVAAFLASRKA"
     assembly_gap    5408..5441
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5442..5836)
                     /locus_tag="PLY93_04305"
     CDS             complement(<5442..5836)
                     /locus_tag="PLY93_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68731.1"
                     /translation="MKLFYRGTTLFTFFLLLVTSACQEKSKGELGSELIEAVREGKAS
                     QVRSLIEAGASLSVRDENGEILWRVALEHYNKRTWEVLTENHIDLSEPDSGASYAIHV
                     AAANGLSDAAERILKLDAGKVRLNANGDTP"
     gene            complement(5899..>6580)
                     /locus_tag="PLY93_04310"
     CDS             complement(5899..>6580)
                     /locus_tag="PLY93_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68732.1"
                     /translation="TVSAGTPTASNGNKTFTYSLSGLTVSTGYATCVTTGAKDVAGNA
                     LASSYTATWTTGVGGQNFAIQDGGFEAGTDGVQPTGWTGVVGATVSSAEARTGTKSLL
                     FTSNPGGNSTAFDIPNSLKNVRGGNTKFVFWIKGTADKALVFTFNGTNTGQRRCGPIT
                     TSDVTASNSSAYGGPTGEINTAGAWRKCTIDLTSIITDFDAANPIRLRVGSSGNYNVY
                     LDDFEFEP"
BASE COUNT         1612 a         1618 c         1473 g         1843 t
ORIGIN      
        1 agtgcagtat aaaatttatt ctcgtcaaaa tcgtcgttac ttacgggtaa ctctggcaaa
       61 actgcgagcg tgtcgttatt cccacaaagt gtctgtacac gcaacacata attatactca
      121 agcacggtgg ccttacttgc ttcgaggtat tctctcagtt tttcagtgtc tctctcaggg
      181 ttgcattcgc gctccaatac actcagtagc ctctgcgcgc ggcggttcga tagatgtaaa
      241 ttttcttcca ttgccgagac atagcggcgc cggaatgatt ttttattaat ctgaaagaac
      301 gcttcagcca agcgcgcttc tttaaggcgt agcgcaaaaa gttcatggta tgccgcccgc
      361 tttagtttat gagcgccagt ttcgcgaata aattttacat aaagcgcttc ggcttcacgc
      421 ggatcatttt ctgttaattg ttgtgcacgt cggtaaagag atgaccgtgc ttcaacgaca
      481 gccaccgaaa agaaaagaaa taaagcaagt accggtatct tttttaatga aattgcgaat
      541 cccccatgat ggcgacaaag tcgttttcca aatcaacagc cttgccgtgc aggcattccc
      601 cttgcggaac aacccccgat ataaaaacct cactctgcgc atgcgagcaa gtactcgacg
      661 ccagcttacc gcttaaaggg cagacgcgca agcgcaccat accgggtttc attttgaatg
      721 gaatgggggc gaaatctttc cttaaaaaat ccgccgccaa ctttgacgcc aatgccgaac
      781 cggttgcccc gggtatcgaa tagcgcatgt catcgtaacc aaaccaaacg gcgacgatca
      841 catcctttcc gccaccgaca aaccacaaat cgcgggagtt tgaggtggtg cctgttttac
      901 ctatcacgcc ggctaacgaa gtgcccgaaa acgatccaga gcttttaaga agtgaaatca
      961 tcaattcggt ttccgccgcc gagagaagtc tttctttggg ggcttgtttc agtcccaact
     1021 gatgggcgtg cgtgtttttc aaatctatga ctcgatctgg ggtttcaagg cgcgttagca
     1081 aaacagggtt attcaaataa ccttgatttg caaaaaccga aaaggcgcgc gccatctcta
     1141 aaggcgaaag cgaaatagtg ccgatggcga cggtttcatc gtaagcaaag cgttcgcttt
     1201 gctgcgtttc atcttggaag aaaaaatcgt aaaaaagttc ggtcactgcg tcgcggccaa
     1261 gcaaccgccc caccctaatt gcggggatgt tgcgcgattt tgccagtgcg gtgcgcgctg
     1321 tgattggccc ttcaaagtca ccgttgatat tttccggcgc ccacgttcgc gaaccggaac
     1381 cagcgatgta cggcgaatcg tcgataagcg tcgccggatt tatctggtgc gtactcaacg
     1441 cttttgcgta aataaaaggt tttattgcgg aaccggtttg cctacgcatt tttacagtgc
     1501 ggttgagttg attgttgctt tcaaagccgt cgccgcccac catggctaaa atttcacccg
     1561 tggttggctt tatagcaatg agcgcagcct gtaataacgg ttcggcattc gtgacaggag
     1621 taaatccccc caagtccagc aacagccccg cgtcaaacgc cgcgtggcgc acgctgttct
     1681 tcaaacggac gcttgcgccg tcgctgcttc taattctacc atggaagcgt ttgcgtgcag
     1741 aaacaaggtg ggtatgcaac aattcttctg cccgccgctg cattgccgca tcgattgtag
     1801 tgtaaacctt ggccgaacct tggctctccg acgacaagat attacgctgc gataaaaatt
     1861 cacgcgcgta ctcagctggc cataaaccaa gacgagattt ttcaccgaac gccgtttcgc
     1921 tcgcaaatcg ttctttttgt tcgctcaccg aaaccatcgc ttgattccat tggtgtacag
     1981 aaagagaaag aatccccgtc gagcgcattc cttcaaaaag tgcattcatc cgcgcttgta
     2041 agcgggcgtt attttttatc ggagaaagcc gctcgggcga tgggatcaaa gccacaatcg
     2101 ctaaaaattc attgggcgac aggtctttca ctggcttgga gaaataaaat ttagctgccg
     2161 aggcaaaaga atacgtaccg tggcctaaat agacgttgtt aacgtagagt tcaagaattt
     2221 cttttttcgt taaaacattt tctaaagcac gcgctaacct tgcttcgcgg acttttctaa
     2281 aaatactttt ctcgcggttt tgcagcatga tgcgcgccaa ctgttgcgta attgtcgacg
     2341 cgccttgcgc atagcgcagg cgcagaagat tgttcaaaaa agcgcgcgcg atggcggtgt
     2401 attcgacgcc gtggtgtttg taaaatcctc tgtcttcgga atataaaaga gcttgaattt
     2461 gccacggctg aaatgcactc aatgtaattt tatcgctttg cgaaactccc agcttggtga
     2521 gttcatgccc cgatcggtcg agcacaacgt cggtttgtcg cgcttttaat aaacgcaccg
     2581 cctctttggc ttcatcgttc gatgcgggaa ttgcctgcca tataaaaaga gtgaatccca
     2641 gaataaaaag aaatgcgaca ccggagaaaa gtaatttcgc ccaagcgtta ttcggttttg
     2701 aaaaagttaa attttctttc agcgtaagaa gtttctcttt taactgcggc caggttggga
     2761 gtttagtctt agtataaact tctctttgtt ctgtctggct gctcaaacta ttttcgtaca
     2821 acgaggcgtt ttgttctaaa ggaggggggg gagctggcgc ttgaaaattt tgcgtcgacg
     2881 tttgtgtgac cggtggcagc gtgtgcacac ggtgagtttg cggtattcca cgttgttcgt
     2941 tgcgcaaaga tgctaccttt gccgtaagaa gtttggtaag ctttccctcg cggttgtcgt
     3001 atccatggct catgccgcag tgggggcagt cagcggttaa aatactatac gacgttaagt
     3061 tgagttgcgg tgaaaatggg cgggcgcaac ggcggcacgc atgttcaaat gcgtgtaaca
     3121 tacgcttact ttagctcaaa taaatcgcgg agatgttttt tttgggtgat ttcaatcgat
     3181 tcgacggtat aacgcaacga tttaaccgtc cgccgcagat attttttata cgtctcaata
     3241 aaatccattt ttttatcgta aaaaggaatg gtcttcaagt cgactttttc cttttctttg
     3301 aaagactcct gtcctctcgg gtcgatttca gattcgtagt aaataacaac catgtcgtcg
     3361 ttccggtcta ctttggtttc gtttttttga gagggatctt ttgactcgtc gttatcaaag
     3421 aacaaaggag tattattgat cacttcgcgt ttttcacgtt tgtattcttt tccaaaaggg
     3481 ttggttctct caaaaaccag tttaactttg cttagcttcg ccgctttttc atcgccggaa
     3541 ggataaagaa caatccgccc atcgtacaaa taacgtaacg aaattgtttt tatcgattct
     3601 cccgatgcaa tttctaaact taaaccgcca tcggcttctt tttgcatgcg gggggctgct
     3661 ttaagcgcca gttgagcaat ttctcggtgt aaattatcga tttcaatatc tccataaccg
     3721 gtttcttcag ctcttctttt ttcttcggct tgctgcgcca aaatttgagg gtcgataatt
     3781 ttttgtgccc aaagaactcc taaaagaccc gcgacagcaa agaaggaaat tagccatagt
     3841 tttttcttaa acatttttta cccgcttctt tatattaacg accaaaaata tgccatcctt
     3901 taacttgcct ttatgctttt cgcgacgcta aaaacgcagc aacgtcttcc gcgtggcgtg
     3961 cgtgcgctaa atcttccgcc ataagccctt tcttattctt aatttttgcc agcgcacgcg
     4021 acttaattaa aagatccact tttgccatat tgccttcgtg cgcggcaaac atgagagcag
     4081 tccatccatc aaggtcttga tcgttaaccc gtgcccccgc tttcaaaaca agcgccatca
     4141 tttcggtatt cccccgccac gtcgccatga tgagggcggt gcgtccgctg cggtggcgca
     4201 agttcacatc gggccggtat tgcatcagca tgtcgaccat ggaggcatcg cctcgaaaga
     4261 gagcaatcat gagcggggtg taaccgtcgc ggtcttgtaa attgacacgt gctttccgat
     4321 ctaaaaggaa ttttgccaca tcgtttgcgc ccgagtttac cgcgtacatc agcgcagtgt
     4381 atccttcttc gtttttgagg tcgatattga tattcgactc taacgcccgt tcggcgcggg
     4441 cgacatcgtt tgaacgagca gccataacta attctttatt atttttagga tcaaccgcac
     4501 cggcgccgac taaaattttc accacttcgt cgtaatcttt aatcgccgcc cagtatgcgg
     4561 cagtacctcc gttgtcggcg gtggcattga catcgacacc ggcattcacc aagcgccgta
     4621 ccatttcgac cgaagctaaa atgagcggcg tgtaaccaaa gttattctta cgtttgatat
     4681 ccgctttgta tttaatcagt tcatcgaaga tgcgcactcg attttcttga gctgcataaa
     4741 aaactgcaga atagccgtct ttatcgcggg cattcacttg tgctcctctc tctaaaagtt
     4801 ctttgagcat ctcataattg ccaaatgcag cggcgattaa gagcggtgtc tgtaaatctt
     4861 tatcggtgta gttaacatcg gcatattcat tgagcgattt tctaaacaca tctccatttg
     4921 ccgtgcgtat cgccagcaca agacgacgac tcgctaaagc gtttaacgcg cctttcgagc
     4981 gtaaatactc tatcatcagt ttatcggcca gctcaactgc gtaggaaagc ggcgaatagc
     5041 ctttctcgtc gaccacgttt acattcgcgc ctcggttgac tgcccccatc gctttcatta
     5101 agtttttctc ttgcgttgct tgcaataact ctttacttgc agcgggattt tcagcgccaa
     5161 agctttcgag taaacgagca atatcggtaa aattatcgaa ccgcgcttgt gaaacggcgg
     5221 tgtggccata ataatttgca gcgggcaccg aaggtttata ttttagaatg agctcaaccg
     5281 ctttaatatt acccgagcga gcgctttcga tgagtgcagt gtcgccgaat ttattacgct
     5341 cgttcgcttc ttgaaagaaa tcagacatca ccgcgaggac attctccatg ccataacgcg
     5401 ctgcgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggagtgtcg ccattcgcgt
     5461 tcaaacgtac tttccctgcg tcgagtttca aaattctctc tgccgcatcg gacaaaccgt
     5521 ttgctgccgc aacgtgaatg gcataacttg cccccgaatc gggctcggac aagtcgatgt
     5581 gattttcggt gaggacttcc cacgttcttt tattgtagtg ctcgagcgct acgcgccaca
     5641 atatttcacc gttttcatcg cgcaccgaaa gcgatgcccc tgcctcaatt aaactacgca
     5701 cttggcttgc tttgccctcg cgcaccgctt cgataagttc cgaccccagc tcgcctttac
     5761 ttttttcctg gcaagcggat gtaaccaaaa gcaagaaaaa agtgaagagg gttgttccac
     5821 gatagaaaag tttcatgagc cactttcttt gaatgtcaaa agctctgtca acgagaaagt
     5881 ggagtgtgtg tgcaattgct atggctcaaa ttcaaaatca tcgagataga cattgtaatt
     5941 acctgaacta cctactctga gtcgaatagg attcgccgca tcaaaatcag taataattga
     6001 cgtaagatca atggtacact ttcgccatgc acctgctgta tttatttcac ctgttggccc
     6061 accgtatgcg ctactattac tcgcggtcac atcacttgtg gtaattgggc cacagcgacg
     6121 ttgtcctgtg tttgttccat taaatgtaaa tacaagcgcc ttatcagcag ttcctttaat
     6181 ccagaagaca aatttagtat taccacctct tacatttttt aggctatttg gaatgtcaaa
     6241 tgcggttgaa tttccaccag gattagaagt aaaaagcaaa gattttgttc ctgtacgagc
     6301 ttctgcggac gaaacagttg ctccaacaac accagtccat cctgttggtt gaacaccgtc
     6361 tgttccagct tcaaagccgc catcttgaat cgcaaaattt tgtcccccaa cacccgtagt
     6421 ccacgttgcg gtgtacgacg atgccagcgc attgcccgcc acgtctttgg ctcccgtcgt
     6481 aacgcaggtg gcgtaacccg tactgactgt taagccactt aacgaatacg taaaggtttt
     6541 gttgccgtta cttgctgtcg gtgtacccgc agaaaccgtt
//
LOCUS       DAOMPD010000087         6576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5008_length_6576_cov_2.414047, whole genome shotgun sequence.
ACCESSION   DAOMPD010000087 DAOMPD010000000
VERSION     DAOMPD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_6576_cov_2.414047"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLY93_04315"
     CDS             complement(<1..298)
                     /locus_tag="PLY93_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRP68733.1"
                     /translation="MDKIKDFLASRRATTVLAVLLFFAFSYLSYGLYIRFDVSSAGTL
                     RISQSSKKLLRELPEKATIELFVSNDLPDEAVLIARKVRDFIQEYVNASRGHIKL"
     gene            complement(298..1047)
                     /locus_tag="PLY93_04320"
     CDS             complement(298..1047)
                     /locus_tag="PLY93_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP68734.1"
                     /translation="MQALSKLDIHCPNAKTVFRREFANYFNTPIGYIFLGIFILLVNF
                     LFFFITRYFDRGYSLQGLFDMLKLVYLAFIPVVTMRLWAEERRSGTVELLLTLPFSEM
                     ELILGKYFSAFLFLGIALASTLLLPLMTYYVGAPDTMVVIGSYLGAWLSGSAYIALGL
                     YISWLTRDQITAFLLSFLACLFIYLLGYQPVLQFFGPFKELLGFLSVSWHADSLSAGI
                     LDTRDILYYISFATLFLYLNKVSIEKFRRGN"
     gene            complement(1058..>1241)
                     /locus_tag="PLY93_04325"
     CDS             complement(1058..>1241)
                     /locus_tag="PLY93_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68735.1"
                     /translation="LTLLSTSDAETRFGAETSTGFNEKLFQLCVSEKWTLREMFAQKQ
                     SIEDVFAELTQKPKDN"
     assembly_gap    1242..1274
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1275..1951)
                     /locus_tag="PLY93_04330"
     CDS             complement(<1275..1951)
                     /locus_tag="PLY93_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP68736.1"
                     /translation="MIAVSSLEKSFGPYQAVRQVNFSIGKGEVVALLGPNGAGKTTTM
                     RMLTGFYEPTSGQVTIGGLDVVKDRKAVQSMIGYLPEGANSYPDMLVGEFLNFIAESR
                     SLSDEAAQKGIAKAIEVTDLKDKIHVPIMQLSKGYKQRVGLAAALLHDPQVLILDEPT
                     SGLDPNQIHEIRQLIRNLAKEKTIILSTHILKEVEDTCERAIIINNGRIVLDERLDKI
                     QRRKEGH"
     gene            complement(1991..3016)
                     /locus_tag="PLY93_04335"
     CDS             complement(1991..3016)
                     /locus_tag="PLY93_04335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRP68737.1"
                     /translation="MIRKMILLVTFLSSALAAGEKKAITINAVGDMVLGTLYPEKRIP
                     QNPYETFFEPIQQYLSGADILFGNFESTLTHHSKTSKNTASKHVFAFRSPVAMAAVLK
                     RAGFTVLNTANNHSGDFGAQGFADTMSNIKAQGILAIGHKDQIGYKTIEDTRIAFIPF
                     SYLRAHSSVKDLPAMRTLVKKAREKADIVVISCHWGGEGEKFLHTKNQTEIFFGENRG
                     NLPEFAHAAIDAGADLILGHGPHVVRALELYKGRLVVYSLGNFIGYGALSSRGKTGLS
                     LVLSIQLDNEGKFISGKIIPMHMPASSLPRYDASKKTIRLMQTLTTADFPRTPLTIDD
                     DGKITIK"
     gene            complement(3009..3746)
                     /locus_tag="PLY93_04340"
     CDS             complement(3009..3746)
                     /locus_tag="PLY93_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68738.1"
                     /translation="MLKNAATPGILIITGPRTKYGSGHNVRMRKAALLLRKKKIDVTL
                     LCLDEGSIVDVPLPYSLCILDRRDSAFNPPFKKKGVCYVAIDNRGKARNEADYTIDLL
                     PHFSMEDNEYQQALTNIILSTAFFDMPSKAQNARVTLHDTFESAKAAADFTLPQEKIS
                     QREFLSRLKSATRPALYFGQAFFEALDLGLHVQLYPISEYHKKLAVDFKNRLNKNPYL
                     LDYLNPNALIRFVDTIQKIYKENKKHD"
     gene            complement(3767..4807)
                     /locus_tag="PLY93_04345"
     CDS             complement(3767..4807)
                     /locus_tag="PLY93_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68739.1"
                     /translation="MKTLILQDWYEENDLLNPSLFALIELLHEADVGFLQNFENKLPH
                     ELADRRVAFYGARLTRKNFDNLEELIALEEKIPWCDYDLVVCNTRGYLRHFRKGIDAK
                     TRIIVYQHDLLAALWRINPKTLNEEETATLLLQQELDLEYAHGINLTIAANFALQKTL
                     SAMFKKNVSLAYPLIDQTIFFPENNVMREYFVALDTVDRERLTRLFACMADKLIVFGE
                     YKHDKLLRELKPDNIYYTGPLTLADQAYYLAGAKAFFCGETHSLSHLPLASLKMGIPL
                     IAHPTQGMTEILEDSDTGVELESASEDELIRHIRHYRNAKESRTKISASVDWLNKESF
                     VKRLKKSIERGI"
     gene            4922..5665
                     /locus_tag="PLY93_04350"
     CDS             4922..5665
                     /locus_tag="PLY93_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP68740.1"
                     /translation="MAESIIRLTSVQKKYAMGDFDVHALRGVTLTIDEGEFTVIMGPS
                     GSGKSTLLNLLGCIDAASEGSVLVCGHDTFNKTEKELAALRRDNIGFIFQAFNLIPVL
                     SVYENVEYALILKGAPEEERSTLVDAIVTQVGLAEYKDHRPNQLSGGQRQRVAIARAL
                     VTRPRLILADEPTANLDSKTGKAILSLMQEMNERYKTTFVFATHDPDVLSFARRAIHI
                     HDGKIDSDTRKAGKRKTLPQKSARKRKSL"
     gene            5662..>6576
                     /locus_tag="PLY93_04355"
     CDS             5662..>6576
                     /locus_tag="PLY93_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68741.1"
                     /translation="MKIKIPTDLKIAFRNVFRNRRRTLLTLSSIVLGCAALINFDGYT
                     NFADWGLREMYIRSQLGHIQIYKKGYRASKGMNPFAYLIANPDEIKKTIATTLGDRLN
                     VVNARLELNGLITKGGQSYNFIAQGIEVEGEEKMLKLENSTSTALNLRRGQYLTKKDT
                     GVVLLGNELCDTLGVQEGDLLSLMGASAVGSLNATDLLWKGCFSTVSKEYDKVAAIMP
                     LADAKRFSATDDVLKLVVVLNDTDMTQKAMADLTRAFQEKGYDLEMVSWYDLADYYKQ
                     VMIFLKGMFMVIATIIVFVVVFSIVNTLT"
BASE COUNT         1731 a         1446 c         1623 g         1743 t
ORIGIN      
        1 tgagctttat atggccgcgc gatgcgttca cgtactcttg gataaagtcg cgtactttgc
       61 gcgcaatgag caccgcttcg tcgggtaaat cgttcgagac aaaaagttca atcgtcgctt
      121 tttcaggcag ttcgcgtaaa agttttttcg agctttgcga aatgcgtagc gtacccgccg
      181 atgaaacgtc gaaacgaata taaaggccgt agcttaaata actgaacgcg aaaaagagga
      241 gtaccgcaag caccgttgtg gcgcggcgtg atgctaaaaa atctttaatc ttgtccatta
      301 gttgcctcgt ctaaattttt cgatgctcac tttattgagg tacaaaaaga gagtagcgaa
      361 agagatatag taaagaatgt cgcgcgtatc gaggatgcct gccgaaagcg agtcggcgtg
      421 ccacgaaacc gacaagaagc ccaagagctc tttaaacggc ccgaaaaatt gtagtaccgg
      481 ttgatacccc aacagataaa taaaaagaca tgcaagaaaa ctcaaaagga acgcggtgat
      541 ttggtcgcgc gtgagccatg agatataaag ccccaacgcg atgtacgccg aacccgaaag
      601 ccaagcgcct aaatacgaac caatgaccac cattgtgtcg ggcgcgccga cataatacgt
      661 cattaagggg agtagcagtg ttgacgcgag ggcaatccct aaaaataaaa acgcagagaa
      721 gtattttcct aaaatgagtt ccatctcgct aaaagggagg gtgagtaata attccaccgt
      781 accgctgcgc cgctcttctg cccaaaggcg catcgtcacc accggtataa acgccagata
      841 gacgagcttc agcatatcga aaagcccttg tagcgaataa cctctgtcga agtagcgggt
      901 aataaagaag aaaagaaaat ttaccaacag gataaaaatt cccaagaaga tatacccgat
      961 gggggtgttg aagtagttgg caaattcgcg gcggaaaacg gttttggcgt tagggcagtg
     1021 gatgtctaat ttactgagtg cttgcatttt cttatcctta gttgtcttta ggtttttgcg
     1081 ttagttcggc aaagacatct tcgatgcttt gcttttgcgc aaacatttcg cgcagcgtcc
     1141 atttttcact cacgcagagt tggaacagct tttcattaaa acccgtcgat gtctcggcac
     1201 caaagcgtgt ttcggcgtcg gaggtggaaa gcagagtgag tnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnntcgtgg ccttctttac gccgctggat tttatcgagc ctttcatcga
     1321 ggacgatgcg gccattgttg atgatgatcg cgcgctcgca agtatcttcg acttctttta
     1381 aaatgtgtgt gcttaaaata attgttttct cttttgccaa attgcggatg agttgacgga
     1441 tttcatgaat ttggttgggg tcaagccccg aggtgggctc gtctaaaatc aagacttgcg
     1501 ggtcgtgcaa gagcgctgcc gcgagtccca cccgttgttt ataccctttc gagagctgca
     1561 tgataggcac gtggatttta tctttaaggt cggtgacttc gatggcttta gcaatccctt
     1621 tttgcgcagc ttcgtcgctg agcgaacggc tttcggcgat aaaatttaag aactctccca
     1681 cgagcatgtc ggggtatgag tttgcgcctt cgggtaagta gccaatcatg ctttgcaccg
     1741 ctttgcgatc tttgacgaca tcgaggccgc cgatagttac ctgccctgaa gtgggttcat
     1801 aaaaaccggt aagcatacgc atggtggtag ttttccccgc accgttgggg cccaagagag
     1861 caaccacctc gcctttaccg atagagaaat tgacttgcct caccgcttgg taaggaccga
     1921 aacttttttc aagagaactc acagcaatca tgaagcgagc ttaattttaa atgctggtcg
     1981 tcaagccttt ctacttaatt gttatcttgc cgtcgtcgtc gatggtaaga ggtgttcggg
     2041 ggaagtctgc cgttgttaaa gtttgcatca ggcggattgt ctttttagag gcgtcgtagc
     2101 gtggcagcga agaggcaggc atgtgcatcg gaataatttt acccgatata aactttcctt
     2161 cgttgtcaag ctggatcgaa agcacaagcg agaggccggt tttgccgcgc gaagagagcg
     2221 caccgtagcc aataaaatta ccgagcgagt acacaacaag acgtcccttg taaagttcga
     2281 gagcgcgcac gacatggggg ccgtgaccta aaattaaatc ggcgccggcg tcgatggcgg
     2341 cgtgcgcaaa ttcaggaaga ttgcctctgt tttcaccgaa aaatatttca gtttggtttt
     2401 tggtgtgcaa gaatttttcg ccttcgccgc cccagtggca actaatgacc acgatgtcgg
     2461 ctttctcgcg cgcttttttt acgagagtac gcatcgccgg taaatctttt acgctcgaat
     2521 gtgcgcgcaa ataactaaat gggataaaag caatgcgcgt atcctcgatg gttttatacc
     2581 caatttgatc tttatggccg atggctaaaa tcccctgtgc tttgatattc gacatggtgt
     2641 ctgcaaaacc ttgtgcgcca aaatcccctg agtggttatt ggcagtgttg aggacggtga
     2701 agcctgcacg cttcaagacg gcggccatcg ccaccggcga tcggaatgca aagacgtgct
     2761 tggacgccgt gttctttgag gtttttgagt ggtgtgtgag tgtgctctca aaattgccaa
     2821 agaggatgtc cgcacccgat aaatattgtt ggatgggctc aaagaacgtc tcgtaagggt
     2881 tttgagggat tcgcttttct ggataaagcg tacccaacac catgtcgccc acggcgttga
     2941 tagtgatcgc tttcttttct ccagcggcga gagcgctact taaaaaagta acaagaagta
     3001 tcatttttct aatcatgttt tttattctct ttgtatattt tttgaatcgt gtctacgaaa
     3061 cgtattaaag catttgggtt taagtaatcg agcaagtatg gattcttatt gagtcggttt
     3121 ttgaaatcta ctgcgagctt tttatgatac tcgcttatgg ggtaaagctg gacatgaagc
     3181 cccaagtcga gcgcttcaaa aaacgcctgg ccaaaataga gggcagggcg tgttgcactt
     3241 ttaaggcggg ataaaaactc tctttgtgaa attttttcct gcggcaaagt aaagtccgca
     3301 gcagctttcg cgctttcaaa cgtgtcgtgt aaagtaacgc gcgcattttg agccttacta
     3361 ggcatgtcaa aaaatgcagt acttaaaata atgtttgtaa gcgcctgctg gtattcatta
     3421 tcttccatgc taaaatgggg caaaaggtct atggtataat ctgcctcgtt gcgcgctttg
     3481 ccacggttgt cgatagccac gtaacagacg cctttttttt tgaaaggagg attgaacgca
     3541 ctgtcgcgcc gatctaaaat gcagagtgaa taaggcaaag gaacatcgac gatacttcct
     3601 tcatcgaggc ataaaagcgt cacgtcgatt ttttttttac gcaagaggag cgcagccttg
     3661 cgcatacgca cgttatgccc cgaaccatat tttgtgcggg ggcctgtgat gatgagaatg
     3721 cctggcgtcg cagcattttt gagcatttcc atcggcatag aaatatttat atcccccgct
     3781 cgatagattt tttgagccgc tttacaaaac tttctttatt aagccaatcg acgcttgcgc
     3841 taattttcgt tcgactttcc ttcgcattcc ggtaatgacg gatgtgccgg atgagttcat
     3901 cctccgaagc agactctagt tcaacacccg tgtcgctatc ttctaaaatc tctgtcatcc
     3961 cctgcgtggg gtgtgcaatc agtgggatgc ccattttgag cgatgccaga ggcaaatggc
     4021 tgagagaatg cgtttcacca caaaagaatg cttttgcgcc cgctaaataa tatgcttgat
     4081 cggcgagagt aagtggacct gtataataaa tgttgtctgg ttttaattcg cgcaaaagtt
     4141 tgtcgtgttt atattcgcca aagacgatca atttgtcagc catgcacgca aataagcgag
     4201 tgagccgttc gcggtctacc gtatcgagag cgacaaagta ttcgcgcata acattatttt
     4261 cggggaagaa aatagtttgg tcgatgagtg ggtatgccaa cgaaacgttt tttttgaaca
     4321 tcgcgcttag ggttttctga agtgcaaagt tggctgcgat ggtgagattg atcccatgcg
     4381 catattcaag atcgagttct tgttgtagca aaagcgttgc cgtttcttct tcattgagtg
     4441 ttttgggatt gatgcgccag agcgcagcga gcaaatcatg ttgataaaca atgatacgtg
     4501 tttttgcgtc gatacctttt ctgaaatggc gtaagtaacc acgcgtgtta catacgacaa
     4561 gatcataatc acaccaagga attttttctt ctaatgcgat gagttcctca agattatcaa
     4621 agtttttacg cgtcaagcgt gcgccataaa aagccacgcg acggtcggcg agttcgtgag
     4681 gcagcttatt ttcaaaattt tgtaagaagc ctacgtcggc ctcgtgtaaa agttcgatga
     4741 gcgcaaaaag actgggattc aataaatcgt tctcttcgta ccaatcttgt aaaatgagag
     4801 tcttcacgga cgtgtaagat tcaaaatgga atataaggcg tacactgtat tgacacttgc
     4861 cttgaacaga tgttcaattt tggattgccg cacagactcc gttgaaagtt cggatatttt
     4921 tatggcagag tcaatcatcc gcttaacttc ggtgcagaaa aaatacgcga tgggcgattt
     4981 tgacgtacac gcgttgcgcg gcgtcaccct caccatcgac gagggcgagt ttacagtcat
     5041 tatgggacct tcgggttcgg gaaaaagtac tcttctgaat ttactgggat gtatcgacgc
     5101 ggcgagtgaa gggagtgtgc tggtttgtgg ccacgacact tttaataaaa ccgaaaaaga
     5161 attggcggcc ttaaggcgcg ataacatcgg ttttattttc caggcgttta acttgatacc
     5221 ggtgttgtcg gtgtatgaaa atgtcgagta cgctctcatc ctcaaaggcg cccccgaaga
     5281 agaacgctcc actcttgtcg acgccatcgt tacccaagtg ggtttagcag agtataaaga
     5341 tcaccgcccc aaccaacttt cgggcgggca aagacagcgt gtggccatcg cgcgtgcgtt
     5401 ggtgacgcgg ccacgcctca ttttagccga cgaacccaca gcaaacctcg actccaaaac
     5461 gggaaaagcg attttgagcc ttatgcaaga aatgaacgaa cgctataaaa cgacgtttgt
     5521 ctttgccaca cacgatcccg acgtgttgtc gtttgcacgg cgggctatcc acatccacga
     5581 tgggaaaatc gacagcgaca cccgaaaggc agggaaaaga aaaacacttc cccaaaaatc
     5641 cgccagaaaa aggaaatctt tatgaaaata aaaatcccca ccgatttaaa aattgccttc
     5701 cgcaatgtgt ttcgtaaccg tcgtcgcact cttttaactt tgtcgtcgat tgtcttgggc
     5761 tgcgcagcgc tcatcaactt tgacggctac accaattttg ccgactgggg tctacgcgaa
     5821 atgtatatcc gatcgcaatt ggggcatatc caaatttata aaaaaggata tcgcgcctcg
     5881 aaaggaatga atcctttcgc gtatcttatt gcaaaccccg atgaaatcaa aaagactatc
     5941 gcaacaacgt tgggcgatcg cttgaatgtg gtcaacgcac ggcttgaact caacggcctt
     6001 atcactaaag gtggccagtc gtataatttt atcgcacagg gaattgaagt tgaaggcgaa
     6061 gaaaaaatgc tcaagctcga aaacagcacc tcgacagcgc ttaatctccg acgcgggcaa
     6121 tacctcacca aaaaagatac cggcgtggtg ttgttaggta atgaactttg cgacacgctt
     6181 ggtgttcaag agggcgatct tttaagcctc atgggggcaa gcgcagtggg ttcactcaac
     6241 gccaccgacc ttttgtggaa gggatgcttt tcgaccgtat ccaaagaata cgacaaggtg
     6301 gcggcgatta tgccattggc ggatgcaaag cgattttcag cgaccgacga cgtgttgaaa
     6361 ctggtggtgg tcctgaacga caccgacatg acgcaaaaag ccatggcaga tttaacgcgt
     6421 gctttccaag aaaaggggta cgatttagaa atggtgtcgt ggtatgactt ggcggattat
     6481 tacaaacaag tgatgatttt tttgaaaggt atgtttatgg tgattgcaac gatcatcgtt
     6541 tttgtggtgg tcttctccat cgtgaatact ctcacc
//
LOCUS       DAOMPD010000088         6528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5054_length_6528_cov_3.492198, whole genome shotgun sequence.
ACCESSION   DAOMPD010000088 DAOMPD010000000
VERSION     DAOMPD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_6528_cov_3.492198"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLY93_04360"
     CDS             complement(<1..871)
                     /locus_tag="PLY93_04360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRP68742.1"
                     /translation="MKLASRLSLIEPSPTLAISARANELKAEGKDIVGFGAGEPDFDT
                     PEHIKEAAILALKQGFTKYTAVSGIQDLKKAIVDKFKRDNNLDFQPNQILVSTGGKQV
                     LYNFFLAVLNDGDEVVCPAPYWVSYKDIVSLAGGSMKLIPTTIESGFKINPTVLEKSI
                     TPRTKVFILNSPSNPTGATYTPDEIKALARVLEKFPNVLILTDDIYEKLTYGVPFLNI
                     ASASESLRPRTMVVNGLSKAYAMTGWRLGYAGGPREIIAAMDMLQGQSTTNANSIAQK
                     GAVAALAQSQECVE"
     gene            963..1385
                     /locus_tag="PLY93_04365"
     CDS             963..1385
                     /locus_tag="PLY93_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRP68743.1"
                     /translation="MTGVKYLEESLALSLDFQKLPRIAASGVPLLPVVVQDVLSREVL
                     LVAYVNEEALRLSLAEKRAVFYSTSRGEIWRKGDTSGDVLYLEEVRVNCEQNSLLFLV
                     RKAHAGACHTKNAKGDHRTTCYYRRVVDKELSPVVGVF"
     gene            1411..3012
                     /locus_tag="PLY93_04370"
     CDS             1411..3012
                     /locus_tag="PLY93_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68744.1"
                     /translation="MKLLHEHWGFIFVFAFFSAMLFFFPLKLLSPESYFFSAAIEGYY
                     NLSSTFQTFQNDVLLPDISRAHPNHPIPHYIAGIVFDFTKIPAMDTLRYLNILGALGT
                     LFAFYLCLLQLFQRKSVIIFGLLSLAFSFSFWQEALVAEVHLISLAFIVGAFYFFIRC
                     YKDNERKYYKNFLIATILFAAAVSYHLFASYVSIAIIIAILSKADWRRHWRLYLLSAF
                     IVLFFCLVFYVGFLVWVLKIHSIHEYWGTFFIYKYLQDKYYSGLTWFVVFGKSFLHSI
                     VYGYNQFGVVSQIILVIFLVFGYFSVIKSRWDRPIKVLVFTWPLVYLLMGVILDTRAD
                     GLSVWLFCLPSTIVALCSTFNYLAKNAGAQIYIVVFIAAISGVNFLSTVWPNSQLNRS
                     DFIFIDAVPELMQDSAKNSSEKYKKNILTFVGNQSLTFPELREYVKKNGSENLQIVNC
                     CNRIEEKINKIKEWLSQHSNGYIIDDTFTEWIPKILHDKKISYNLIFGKSSLVPEHWI
                     PSSIYFPTRFGGYRKQLRVWKFISQ"
     gene            3384..3899
                     /locus_tag="PLY93_04375"
     CDS             3384..3899
                     /locus_tag="PLY93_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68745.1"
                     /translation="MRIIDDRYRVTELEIRTRKASAWLVSTALYCDVYALPWNFLSKA
                     KKMGERGGVLAVDALNALFIIERQICPYDKKELKKNRDHIMKIAIKNLSTIKKFSPLW
                     FDLLLALYLNGLKEKIPRGDFTLLAGSQNFDGSWLNDDELTAKVLWILLMEKTGYKPG
                     MPFELTRKSGF"
     gene            complement(3896..4924)
                     /locus_tag="PLY93_04380"
     CDS             complement(3896..4924)
                     /locus_tag="PLY93_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP68746.1"
                     /translation="MPNFFQNAKAGIYFVRSLYGYKRGRAKPLSAVFVVCNRCNLRCK
                     YCNSPFLKERELSLSEIDTLFTNLRNTGIFRLGLTGGEPLVREDIQEIVKLALQKKFY
                     LSMNSNLLLYHERPHIFNDVNFVFTSLDGPPQVHEKNRGKTSQEGVVDAIIDLRRRKK
                     PVVAICVVDQQDSTDIEYLVDLAERHDFKLHFQIRGSLKNRKENVIIRGNLSDSFDDN
                     NYRQIWKFLIEKKKKTHAIANSEQYFKKVIEWDNYHQFTRLSKGKKCAAGFGFLYIAS
                     DGIGYPCSVIKDRYKGINLLKENWIEKFHDKKPCNECLCGPYLEFNLLYKNPIASIAD
                     LMAAYGRG"
     gene            complement(4917..5855)
                     /locus_tag="PLY93_04385"
     CDS             complement(4917..5855)
                     /locus_tag="PLY93_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP68747.1"
                     /translation="MCLISFSKTENYSSKQKIDRFEGRPLWAYVNITDRCSHKCPWCY
                     GGFNANLKNEMRLDDFSIIIEKLQTMGILQVTLSGGEPTEHSEFKSFVQQLSQKNFLM
                     HLATHGGTLASLAPFLIEHGLKQVQVNYQGKELHDAQHGVQGAYTSQREGLAAALREN
                     LEVTATITVGEYNLKKIPQLFQELEGIGLPRFRIWDATGRGNRWRKDIEAVKIFEFCR
                     DEAAKLGFTFVQSYDPEFEGDTGSPCPPMSGLFLHINTKGKLRFCPAVLKEPDVGDFL
                     VHTADELLALNHQNNKRLLDINGGRPWCVARDEAYA"
     gene            complement(5920..>6528)
                     /locus_tag="PLY93_04390"
     CDS             complement(5920..>6528)
                     /locus_tag="PLY93_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68748.1"
                     /translation="YKGVLFALPSIALGLLYLLYNHARFDNPLELGVSYMKLEVVSGV
                     SQRAAMYGNVSLHHFFRNFYSELLRPIDLVRYFPFIEVNPHGFGLFWATPLFGLVFVK
                     ARDLYRVYKNRMSPTDGDRLALAALATVVSMATIIFLIPSSGYMQFGARYTLDFQVFL
                     FIAIGILAGRFHRRILWSLAVISLWIQFIGAVLFLKIFKPMT"
BASE COUNT         1815 a         1390 c         1371 g         1952 t
ORIGIN      
        1 cttcgacaca ctcttgcgac tgtgcaagcg cagccaccgc ccccttttgt gcaatgctat
       61 tcgcgtttgt ggtcgattgc ccctgcaaca tatccatggc ggcaataatt tcacgcgggc
      121 cgcctgcata gcccaaacgc catcccgtca ttgcgtacgc tttggaaagt ccgttcacca
      181 ccatcgtgcg cgggcgcaac gactcggacg cactggcgat attcaaaaac ggaacgccgt
      241 aagtcagctt ctcataaata tcgtcggtga ggatcaacac gtttggaaat ttttccaaca
      301 cacgcgccaa cgctttaatt tcatcggggg tgtacgttgc accggttggg ttggaaggcg
      361 aattcaaaat aaaaactttg gtgcgtgggg tgatgctttt ttctaataca gtgggattga
      421 ttttgaaacc gctttcaatc gtcgtcggta taagtttcat cgaaccgccc gccaacgaca
      481 caatgtcttt gtagctcacc cagtacggcg cagggcacac aacctcgtcg ccatcgttta
      541 agacggcgag aaaaaagtta tacagcactt gcttgccccc cgtcgaaacc aaaatttggt
      601 tgggctggaa atctaaattg ttgtcgcgct taaatttatc gacaatcgct tttttcaagt
      661 cttggatgcc cgacacggcg gtgtacttgg tgaatccctg cttaagcgca agaatcgccg
      721 cttctttaat gtgttcgggt gtgtcgaaat cgggttcgcc tgcaccaaaa ccgacgatgt
      781 ctttgccctc ggctttgagt tcatttgcac gcgccgaaat cgcgagcgtg ggcgagggtt
      841 cgataaggga gagacgtgaa gcaagtttca tagcccgcac tttgtgtttt agatacgtcg
      901 tactctactt tttgagtctt gattaagctt ggcgacctac atacaactcc cgtttagcac
      961 ccatgactgg cgttaaatat ctcgaagagt cgcttgcgct ttcgctcgat ttccaaaaac
     1021 taccgcgcat cgctgcatcg ggtgtgccac tcttgccggt cgtcgtgcaa gacgttcttt
     1081 cgcgcgaagt attacttgtt gcgtacgtca acgaagaggc gctacgcttg agcctcgctg
     1141 aaaagcgcgc ggtgttttac tccacttcgc gcggtgaaat ttggcgcaag ggcgacacaa
     1201 gcggagacgt gctctattta gaagaagtgc gcgtcaactg cgagcagaac agtcttttgt
     1261 ttctggtgcg taaagcgcac gcgggcgcct gtcacacgaa aaacgcaaaa ggcgatcacc
     1321 gcacgacgtg ctactaccga cgggtagtgg acaaagaact ttctcctgtt gtaggagtat
     1381 tttagcaata tcgcaatgcg tcattgcctt ttgaagttac tgcacgagca ttgggggttt
     1441 atttttgtat tcgcattttt cagtgcgatg ctttttttct ttccactaaa gctgttaagc
     1501 ccagaatcgt attttttctc tgccgccatc gaaggatact ataacctttc ctccacgttc
     1561 caaacttttc agaacgatgt ccttttacca gatataagta gagctcaccc taaccaccca
     1621 atccctcatt atattgcagg tatcgttttt gattttacaa aaattccagc aatggatact
     1681 ttacgttact taaatatact tggagcattg ggtactcttt ttgcatttta tttatgctta
     1741 cttcaattat ttcaacgaaa gagcgtaata atttttggtc ttttgtcgtt agctttctca
     1801 ttttcttttt ggcaagaggc gttggttgct gaagtacact taatatcgct tgcctttatc
     1861 gttggggcat tctatttttt cattcgctgc tacaaagata acgaaagaaa atactacaaa
     1921 aattttttaa ttgcaacaat actttttgcc gctgcggttt cataccacct ctttgcctct
     1981 tatgtttcta ttgcaattat tattgctatt ctatcaaaag cggattggcg ccgtcattgg
     2041 cgcctttacc tactttcagc gtttattgtt ctttttttct gtcttgtttt ttatgttgga
     2101 tttcttgttt gggttcttaa aattcattcg atacatgagt actggggtac cttttttatc
     2161 tacaagtatc ttcaagataa gtattactca gggcttacat ggtttgttgt ttttggaaaa
     2221 tcttttttgc attctatagt ttatggttat aatcaattcg gcgtggtatc ccaaatcata
     2281 cttgttattt ttcttgtatt cggctatttt agcgtaataa agtcacgatg ggatcgcccc
     2341 attaaagttt tggtttttac atggccatta gtataccttt tgatgggcgt tatccttgat
     2401 acacgtgcag atggtttgag tgtgtggctt ttttgtttgc cttcgacaat tgtcgcatta
     2461 tgtagtactt ttaattattt agcgaaaaat gccggcgctc aaatatatat cgtcgtattc
     2521 attgcagcga tttcgggagt aaatttttta tctacggtat ggccaaattc acagctcaac
     2581 cggagcgact tcatttttat cgatgctgtc ccagagttaa tgcaagacag cgctaaaaat
     2641 tcatcggaaa aatataaaaa gaatatattg acctttgtcg gcaaccaatc gctgaccttc
     2701 ccagagttac gcgaatatgt aaaaaaaaac ggatcggaaa atttacaaat tgtcaattgc
     2761 tgcaatagaa tagaagaaaa aataaataaa ataaaagaat ggctgtcgca acattctaat
     2821 ggctatatta tcgatgatac atttacggaa tggataccca aaattcttca tgacaaaaag
     2881 ataagctata atcttatttt tggaaaatca tcgcttgttc cagagcattg gataccgagt
     2941 tcaatttact ttcctacgcg ctttggagga tataggaagc agttgcgtgt ttggaaattt
     3001 atttctcaat aaaagataaa tctgaattgt tttttgctgg atatattaaa taggtttata
     3061 aaaattgtat ttactattaa ataggaagga gtagaataat tatgctattt tgtagtcatt
     3121 ttttcttaaa atttcgaaag aattattctt atattagttc tttcctaagt ctactacttt
     3181 ttttattgct tgtgtcgaat atacaagcag tagacgtaca aattaacaaa gcaatttctt
     3241 attttcaaaa aaaatctagc tctatagcgc ctacctctat cgtcactctc gatttagtaa
     3301 ttagaaacta ccatctacca atcgatacat tatccgtacg acgtaagctt ataaaaactg
     3361 cccccgataa atggcggtat ttgttgcgta ttatcgatga tcgttatcgc gttacagagt
     3421 tagaaattcg aacacgtaaa gccagcgcat ggcttgtttc tacagcgctt tactgcgatg
     3481 tatatgcact accatggaat tttctgagta aagcgaaaaa aatgggggaa cggggaggag
     3541 tgcttgctgt ggatgcactt aatgcattgt ttattattga acgacaaata tgcccgtacg
     3601 ataaaaaaga gctaaagaaa aatcgagatc acattatgaa aatagcaata aaaaacctct
     3661 ctactataaa aaaattttca cctctttggt ttgatctttt attggcgttg tatcttaatg
     3721 gactaaagga aaaaatacct cggggggatt ttacacttct tgctggttca caaaattttg
     3781 atggttcttg gctgaacgac gacgaattga cggcaaaagt tttgtggatt ctccttatgg
     3841 agaaaacggg atacaaacca ggaatgccgt ttgagctcac gcgcaaaagt gggttttagc
     3901 ccctgccata cgctgccatt aaatccgcaa tcgacgcgat ggggttttta tagagcaagt
     3961 tgaattcgag gtaagggcca cacaaacatt cgttacaagg ttttttatca tgaaatttct
     4021 caatccaatt ttctttaagt aaattaatcc ctttgtaacg atctttaata acagaacatg
     4081 ggtacccaat accatcggat gcgatatata aaaatccgaa acctgctgca catttcttcc
     4141 ctttcgataa gcgtgtgaat tgatggtaat tatcccattc aatcactttt ttgaagtact
     4201 gttctgagtt agcaatggca tgtgtttttt tcttcttctc tataaggaat ttccaaattt
     4261 gacgataatt gttatcgtca aaagaatcag aaagatttcc tctaataatc acgttttctt
     4321 tacgattttt taatgaacca cgaatctgaa agtgtagttt gaaatcgtgc cgttcggcca
     4381 aatcgacgag atactcgata tcggtagaat cttgctggtc gacgacacaa atagccacca
     4441 ccggtttttt gcgtcgacgc agatcgatta ttgcatcaac gacgccttct tgcgacgttt
     4501 tgcctcgatt tttttcgtgt acctgcggtg gtccatccaa gctagtaaaa acaaaattaa
     4561 catcgttaaa aatgtgtggc ctttcatggt agagcaaaag gttggagttc atcgatagat
     4621 aaaatttttt ttgcagggca agttttacaa tctcttgaat atcttcgcgt acaagtggct
     4681 ctccgccggt gaggcctaaa cgaaaaatac cagtattgcg caaatttgtg aagagggtat
     4741 caatttctga aagcgacaat tcgcgctctt ttaagaaagg cgaattacaa tatttgcagc
     4801 gcaagttgca gcgattacat acgacaaaaa ccgccgacaa cggttttgcc cgcccgcgtt
     4861 tatagccata aagactacgg acaaaataaa ttcccgcttt ggcattttgg aaaaaattag
     4921 gcatacgcct cgtcgcgggc aacgcaccat gggcggccac cattgatgtc caaaaggcgc
     4981 ttgttatttt ggtggtttag cgccaacagt tcgtccgccg tatgtactaa aaaatcgccc
     5041 acgtcgggtt ctttgagtac ggcgggacaa aaccgcaatt tacctttggt gttaatgtgt
     5101 aaaaaaagtc cagacatcgg agggcatgga ctccctgtgt cgccttcaaa ttcaggatcg
     5161 tacgactgca cgaaagtaaa tcctaacttg gcagcctcgt cgcggcaaaa ctcaaaaatt
     5221 tttaccgctt caatatcttt acgccaacgg ttgccgcgcc ctgtggcatc ccagatacga
     5281 aaccgcggaa ggccaatacc ttcgagttct tgaaaaagtt gtggaatttt cttcagatta
     5341 tattcgccga cggtaatcgt cgccgtcacc tctaaatttt cgcgtagcgc cgccgcgagc
     5401 ccttcgcgct gcgacgtgta cgcgccttgc acgccgtgct gcgcgtcgtg caactcttta
     5461 ccttggtagt tgacttgcac ttgcttcaag ccgtgttcaa ttaaaaaagg cgcgagtgaa
     5521 gccagcgtac cgccatgcgt cgccagatgc atcaaaaaat tcttttgaga aagttgttgt
     5581 acaaaacttt tgaactctga atgttcggtg ggttcgccgc ctgaaagcgt cacttgtaaa
     5641 atccccatgg tttgtaattt ttcaataatt atagaaaaat cgtctaaccg catttcattt
     5701 ttgaggtttg cgttaaaccc accataacac cacgggcact tatgcgaaca acggtcggtg
     5761 atattcacat acgcccaaag aggacgtcct tcaaaacggt cgatcttttg ctttgaagaa
     5821 taattttccg tttttgaaaa agagatgaga cacatacgct atggttaata gatgcgttgt
     5881 tgcattcacc ggcaagaata aattatattt atgtacagct caagtcatcg gcttgaaaat
     5941 tttgagaaac agcaccgctc ctataaactg aatccatagc gaaattaccg caagagacca
     6001 taaaatacgc cgatgaaacc gccctgcgag tattcctatg gcaataaaaa gaaacacttg
     6061 aaaatccaac gtgtagcgcg cgccgaattg catataccct gagctaggaa ttaagaaaat
     6121 tatcgtagcc atggatacta cagtggcgag cgcggcaagg gcgagacggt cgccgtcggt
     6181 gggggacatc cgatttttat agacacgata caagtccctt gcctttacaa aaacaagccc
     6241 aaaaagtggc gttgcccaaa aaaggccaaa gccatgcgga ttaacttcga tgaaaggaaa
     6301 gtagcgcacc aaatctatcg gtcgcaagag ttcactgtag aagttgcgaa agaagtgatg
     6361 gagagaaaca tttccgtaca ttgctgcgcg ctgagaaact cccgatacga cttcgagttt
     6421 catatacgaa acgccgagtt cgagtggatt atcaaaccgc gcatggttgt agagtaagta
     6481 caaaagaccc agtgcaatgg aaggaagcgc aaaaagcaca cctttata
//
LOCUS       DAOMPD010000089         6522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5059_length_6522_cov_5.088449, whole genome shotgun sequence.
ACCESSION   DAOMPD010000089 DAOMPD010000000
VERSION     DAOMPD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_6522_cov_5.088449"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..812
                     /locus_tag="PLY93_04395"
     CDS             387..812
                     /locus_tag="PLY93_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRP68749.1"
                     /translation="MLFEKMQKESLEARKARDTAKASVLTTALSQIKALAIDDGHRAV
                     TDADVLKIVRQFLKGVEENLSLAAQGKMDKTRAEQFQLEKNVLLSYLPKQMSADEIKK
                     AVLASEAKSIGDAMKYLKTNHDGEYDGKLASTIIKEVIQ"
     gene            809..1609
                     /locus_tag="PLY93_04400"
     CDS             809..1609
                     /locus_tag="PLY93_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68750.1"
                     /translation="MSYLWHALINKWNLLVLGGSLVAGLFFTWTNALWYPVIFILETV
                     YLVGAATNKRFQRAVDAIEGGEKEKDEVRRLRNAIYGSQSPMKSKMSTTENLIRQIKQ
                     NTRQNSKASVQSSEAMLSGLGELESNFVRLLNGYCRLDDMQKTAGKTHETELSKLKEE
                     LSALPTDAAQETRDALAKRIELVEKRAALVTDAEINKKKLIAQVEMVEETLKYLRDQS
                     VQVFDPQLIAKQVEAITTKMETTRETMLEIEKFSSGMDFTMQQARIRE"
     gene            complement(1619..3484)
                     /locus_tag="PLY93_04405"
     CDS             complement(1619..3484)
                     /locus_tag="PLY93_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP68751.1"
                     /translation="MISPQIAKAARKSMLFRHVDRNTQQRILKKFEIQRIEKGDFLFR
                     SNEKSNFMYVVVEGKLRIEKTNLKSENAKLIKVATLRTGEIVGEVALLTNGRHSSQAR
                     ATTDATILRIGKSKLQEVLRHHPLILENLTRIEAMRLRENNTREKTLQKELGFILHLY
                     FTTPSDFAAAESGVTAALNIDKSKSCLVLKKQFLKKTSTANLRNIISARLLKFPSVYL
                     SVNTALHEEKILTTLLHMADRLVVTFSEDRDSITKAAAFLSRKDAKYHAVYATTYTIA
                     VHRPLRENLLRSEMEKILRLKLSFRLNLGNHYWETNDHRAVLIQCRTIARAITDSSQG
                     VAFGGGGARALCEIGVLSELERHRIDFDHVAGTSMGALIAALYAQGLTSREISERFQK
                     YLPTDKGLLSYNLPFVSFFKDRNVNRILRSLFGKMRFEDLPKPLTVIAADLISGKEVR
                     IEKGLIWRAVRASMSLPVIFPPVKYKRYYLVDGGAVNNVPGNVLREKGVRNVLGVNST
                     PLADDTIKEYFASTNLLQLLRPGVKFWSNLRRVLKIIKLFFTRPPILQIANRAMMLEG
                     SELIRQKADEFDLLLSPTVAHFGLFQFERREEIIDAGRKLAHEKSPEIKKLFKAL"
     gene            complement(3477..5684)
                     /locus_tag="PLY93_04410"
     CDS             complement(3477..5684)
                     /locus_tag="PLY93_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRP68752.1"
                     /translation="MRLLTAIAAIAFFACKAPAKTNNLLDYYARIDTAKKPTYLTYYA
                     TAQKLPTSATVGDKIRAYHGAALLQADANTLCREWDCVSQAFDATKEKNRSALFSYAA
                     FHLAELATSDAMFNTALTLLSHTDDAPEALARKISLLKGKILQQSQTALVKEHYHKHI
                     EKFNDAESLYFAAAAFEKQHDITETLKIAFRVLEKPEADFPFSQSGILIRNILHRKIY
                     ELPNNTQRIRLMEALRVAKDKASAKKLYETLDNIELSKEDSQLFTYYASRLLVGASQF
                     QEAARLLQRNNALFFTNDNDKAVLDICERFLKKKQFNTVEDLFTLKNGTKGSVQCLLR
                     SRERQGQYDATTRAIAKTYIENFDSESTLAERLFLRSCLPNTPKHSEEISLSCLEELR
                     GVTSPKSDDEKPLLGASARYYLAREYDRQGALDKARVLLIEIGKHYNDDFYFYRLLEN
                     PLRAQSTLLHENESLNERNIKIFTALQKHDLSAARGIKINSTLTDIEEAVKERMQNLT
                     DTEKIALLLFAADSRSEARELIRSDNKLDVYRSLMALGAVASKPDIALYGVRLYLREK
                     KMRPFLFEIPPFLRDLLYPQTYSAYVEKYGKMRNIEPAAILALIRQESHFFPGARSHA
                     NAQGLMQLLPSTAKLVAKKEKLAHYDLYNPEVNIRLGTAFMQDVMGKYSSDFTHVAIS
                     YNAGPGRYLQWIKKYSNDSDIFIEEIPFRETYSYVRILLTDRIIYRALLGKND"
     gene            5736..>6522
                     /gene="mltG"
                     /locus_tag="PLY93_04415"
     CDS             5736..>6522
                     /gene="mltG"
                     /locus_tag="PLY93_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRP68753.1"
                     /translation="MLQDTMHKFRKPYIVTVAVVLTLVLIFTAVLFLNLRSVSDGSRS
                     LDFPVRSGASLQSVTVQLENANLIHSGFFFKFYMRLTGKATKLKYGVYTLTDGMSVFK
                     IAQVLTSGKTKTISITIAEGLHNRQIADLFVAKKFFVSRDEFLQYASKKSILTKYKIP
                     AQSVEGYLFPDTYFLPPGYSKEKIIEHMIDHFFDKVKTVKNFPTDPRKIHETVILSSI
                     VEREAKLKEERALIAAVFHNRLKDGEPLESCATVQYLFEKPKAR"
BASE COUNT         1762 a         1479 c         1455 g         1826 t
ORIGIN      
        1 cggtcgtcgc gatttttccg atagagaaaa ggcatcaaaa tcactacgcc tgagcctccc
       61 cagccaggtg taaaacgtct gtcgcgtaat accgtgctgc ttgcatccga gcgtaactga
      121 ttttgactct gtgattaaca tcaaaatacc caatcgtgtt cgcacgactc gatcccgaac
      181 cccatttaaa cttttttgta tccgctgtcg gtcaacttct aattttcgca ttgctccccc
      241 ttggtcttgc tgggtagctc aacctagggt ggagcattat taaaacgcac tcaacttgac
      301 cccaaaagtg tcagttatct ctgtgggttg cataagaggg taagaatatc cgcttgacgc
      361 cacttcttga accatcgttc tcacatatgc ttttcgagaa aatgcaaaaa gagagccttg
      421 aggcacgcaa ggcccgcgac actgcaaaag ccagtgtact tacgaccgcg ctttcgcaaa
      481 ttaaagcgct tgctattgac gacggccacc gtgccgttac cgatgcggat gtgcttaaaa
      541 ttgtgcgcca gtttttgaaa ggggtcgagg aaaatctctc tcttgcagcg caaggtaaaa
      601 tggataaaac ccgtgccgag cagtttcaat tagaaaagaa cgtgttgctc tcatatttac
      661 caaagcaaat gtcggcagat gaaataaaaa aagctgtctt agcgtcggaa gcaaagagca
      721 tcggcgatgc gatgaaatac ttgaaaacta accatgacgg tgaatacgac ggcaagttag
      781 cgtcgactat aattaaagag gtaatacaat gagctatctt tggcatgcgc ttatcaacaa
      841 gtggaatctt ttagttcttg gcggttcgct tgtcgccggt ctttttttca catggacaaa
      901 cgcgctctgg tatcctgtca tttttatatt agagacggtg tatcttgttg gcgctgccac
      961 aaataaacgc tttcaacgag cggtcgacgc tatcgaaggc ggcgaaaaag aaaaagacga
     1021 agtgcgccgc ctacgtaacg ctatttacgg ttcacaatcg cctatgaaat cgaaaatgtc
     1081 cactaccgag aacttaatcc gtcaaatcaa acaaaacacc cgccagaaca gcaaagcatc
     1141 ggtgcaatct agtgaagcaa tgctttcggg gttaggcgaa ctggaaagta attttgtgcg
     1201 tctcctcaac ggttactgcc gtttagacga catgcaaaaa acagcgggta aaacgcatga
     1261 aaccgaactt tcaaaactca aggaagaact ctctgcatta ccgacagacg cagcgcaaga
     1321 gacacgcgac gcgttagcta aacgcatcga acttgttgaa aaacgtgccg cgctcgtcac
     1381 cgacgccgag atcaacaaga aaaaactcat tgcacaagtt gaaatggtcg aagaaacgct
     1441 aaaatacttg cgcgatcaat cggtgcaagt gttcgatcca caactaatcg caaagcaagt
     1501 ggaagcaatc accaccaaaa tggaaacgac acgagaaaca atgctcgaga ttgaaaagtt
     1561 ttcatcgggg atggatttca ccatgcaaca ggctcgcatt cgagaatgaa attcttgtct
     1621 ataacgcctt aaataatttt ttaatttcag gtgatttttc atgcgctaat ttccttcctg
     1681 cgtcaatgat ttcttcacgc cgttcaaatt gaaataaccc aaaatgggcg acggttggac
     1741 tcaacaataa atcgaactca tcggcttttt gtcgaatgag ttcggagccc tcgagcatca
     1801 tcgcacggtt ggcaatttgt aaaatcggcg ggcgtgtaaa aaagagcttt ataattttta
     1861 agacgcgcct gaggttgctc caaaatttga caccagggcg cagaagctgc agtaaatttg
     1921 tcgaggcaaa gtattcttta atcgtgtcgt cggccaaagg cgttgaattg acacccaaca
     1981 catttcgaac acctttctct cgcaagacat ttccagggac attgttgacc gcgccgccat
     2041 cgacgagata ataacgtttg tatttcaccg gtggaaaaat aaccggtaag ctcatactcg
     2101 cgcgtacagc gcgccaaatt aaccctttct cgatgcgaac ttcttttcca gaaatcaaat
     2161 ccgcagcgat gaccgtaagt ggcttgggta aatcctcaaa gcgcatttta ccaaaaaggc
     2221 ttctcaggat tcggtttaca ttgcggtctt tgaaaaagga aacaaacggt aagttgtagc
     2281 ttaaaagacc tttatcggtg gggaggtatt tttgaaaacg ctcgctaatc tcgcggctcg
     2341 taagcccttg tgcataaagc gcggcaatca gcgctcccat actcgtgccg gcaacgtggt
     2401 caaaatcgat acggtgccgt tcgagttcag acaaaacgcc aatttcacac aaagcccgcg
     2461 cgccacctcc cccgaaagca accccttgcg aagaatcggt gatggcgcgt gcaatcgtgc
     2521 ggcattggat caacacagcg cggtggtcgt tggtctccca gtaatgattg cctaagttaa
     2581 gtcgaaacga taactttaag cgtaagattt tctccatttc actgcgcaat aaattttcac
     2641 gtaacgggcg atgtactgca atcgtgtacg ttgtcgcgta cacagcatgg tattttgcgt
     2701 ctttccgcga taaaaacgcc gctgctttag taatcgaatc tctgtcttcc gaaaacgtga
     2761 cgacaagacg atcggccatg tgtaaaagcg ttgttaaaat tttctcttcg tgcaaagcgg
     2821 tgttcacaga caaatatacc gaagggaatt tcaataagcg cgcgctaata atgttgcgca
     2881 aattggcggt gctcgtcttc ttcaagaatt gttttttaag aactaaacac gacttcgatt
     2941 tatcgatgtt taacgccgct gtaacccccg attctgcggc ggcaaaatca gagggtgttg
     3001 taaaataaag atggagaata aatcctaatt ccttttggag tgttttttca cgcgtattgt
     3061 tttctcgcaa ccgcatcgct tcgatgcgcg ttaagttctc caaaattaaa ggatgatggc
     3121 gtaacacttc ttgcagctta cttttgccaa tacgcagaat ggttgcatcg gtggtagcgc
     3181 gcgcttggct ggaatggcgg ccattggtaa gcagtgccac ctcacccacg atttcacccg
     3241 tacgcaacgt tgcgactttt attagttttg cattttcact ttttaagttt gtcttttcaa
     3301 tacgcagctt accttcgaca accacgtaca tgaagttact cttttcgtta gagcgaaaaa
     3361 gaaaatcgcc tttttctatt ctttgaattt caaatttttt taaaatacgt tgctgcgtat
     3421 ttctgtctac atgccgaaac agcatacttt ttcgtgcggc ttttgcgatt tgagggctaa
     3481 tcattttttc ctaaaagtgc tcgatatata attcgatcgg taagcaatat acgcacatag
     3541 ctatacgttt ctcgaaaggg aatttcttcg ataaaaatgt cgctatcgtt gctgtatttt
     3601 ttaatccatt gaagatagcg gccggggcct gcgttatacg aaatcgcgac gtgcgtaaaa
     3661 tcactgctat acttacccat tacgtcttgc ataaacgccg tgcctaaccg aatgttgact
     3721 tcggggttat acaaatcgta atgcgctaac ttttcttttt tcgcgaccag tttggccgtc
     3781 gaaggtaaaa gttgcataag tccttgtgcg ttggcgtggc tgcgcgcacc gggaaaaaaa
     3841 tgcgattcct gccggattaa cgctaaaata gccgcaggtt cgatgttacg catttttccg
     3901 tatttttcaa cgtaagcgct gtatgtttgc ggatacaaca agtctctcaa aaacggcggt
     3961 atttcaaaaa gaaaggggcg catttttttt tcacgcaagt aaagtcttac tccatagaga
     4021 gcaatatcgg gttttgatgc gacggctccc aacgccatca agctgcggta cacatcgagc
     4081 ttattgtcgc tgcgtatcaa ttcacgcgcc tcgctacggc tatcggcggc aaaaagaagt
     4141 aatgctattt tttctgtgtc ggttaagttt tgcatgcgct ctttcaccgc ttcttcgatg
     4201 tcggtaagtg tgctattgat ctttattcca cgcgcggcac tcaagtcgtg tttttgaagc
     4261 gccgtaaaaa tttttatatt gcgttcgttg agcgattcgt tttcgtgaag caacgtcgac
     4321 tgggcgcgta gtgggttctc taataagcga taaaaataga agtcatcgtt ataatgcttg
     4381 ccgatttcta tcaaaagaac gcgtgcttta tcgagcgcgc cttgcctatc atattcgcgt
     4441 gcaagatagt accgcgcact cgcgcctaaa agtggttttt cgtcatcact tttgggacta
     4501 gttaccccac gcagctcttc aaggcacgaa agtgaaattt cttcgctatg tttgggggtg
     4561 ttgggtaaac acgaacgcaa aaacaatcgc tcggcaagcg tcgattccga gtcaaaattt
     4621 tcaatatacg ttttggcaat cgcgcgcgtt gttgcgtcgt actgcccttg tctttctcgg
     4681 ctacgcaaca agcactgcac actacctttt gttccatttt ttagtgtaaa caaatcctca
     4741 accgtattga attgcttttt ctttaaaaag cgttcacaaa tgtcgagcac tgctttgtcg
     4801 ttgtcgttcg tgaagaataa agcgttattt ctttggagca gacgcgccgc ttcttgaaat
     4861 tgagatgcac ccacgagcaa gcgcgacgcg tagtatgtaa aaagttgaga gtcctcttta
     4921 ctgagttcaa tgttgtcgag cgtttcgtaa agctttttag cgctcgcttt gtcttttgca
     4981 accctcagtg cctccatgag gcggatccgt tgggtgttat ttggcaattc ataaattttt
     5041 ctatgtaaaa tgttgcgaat caaaatgccc gattgcgaga aagggaaatc ggcttcgggt
     5101 ttttctaaaa ctcggaacgc tattttaagc gtttcggtta tatcgtgttg tttttcaaac
     5161 gctgcggcgg caaagtacaa agactctgca tcgttgaatt tttcaatatg cttgtggtaa
     5221 tgttctttaa cgagtgcagt ttgactctgt tgcaaaattt ttcctttaag taaagaaatt
     5281 tttcgcgcca atgcttctgg tgcatcgtcc gtatgcgaaa gaagggtcag agcagtgtta
     5341 aacattgcat cgctcgttgc caactccgct aaatggaatg cggcataaga aaaaagtgca
     5401 gagcgatttt tctctttcgt ggcatcgaac gcttggctta cgcaatccca ctcgcggcaa
     5461 agggtattcg cgtcggcttg caataaagca gcgccatggt atgcacgaat tttgtcgccc
     5521 acggttgcgc ttgtgggtag tttttgcgcg gttgcatagt atgtcaggta tgttggtttc
     5581 tttgctgtgt cgatacgcgc atagtaatct aaaagattat tggtctttgc aggggcttta
     5641 caggcaaaaa aagcgattgc ggcgattgcg gtaagtagac gcatgcagta tgctcatgga
     5701 gctttacagc atgtcaatcg attgtacgag gccaaatgtt gcaagatacg atgcacaaat
     5761 tcagaaaacc ttacattgtc acggtagcgg tggttttgac gctcgtttta atttttactg
     5821 cagttttatt cttaaatcta cgctctgtat ccgacggctc gcgctcgttg gattttcccg
     5881 tgcgttcggg agcgtcgttg caatcggtga cggtacaact tgaaaatgct aatctaatac
     5941 acagcggctt ctttttcaaa ttttatatgc gcctcaccgg taaagcgacg aaactcaaat
     6001 atggcgtgta cactctcacc gacggtatgt cggtatttaa aattgcacaa gtactcacga
     6061 gcggcaaaac aaaaacgatc agcatcacga ttgctgaagg cttacacaac cgtcaaattg
     6121 ccgatttatt tgtggcaaaa aagttttttg tatccagaga tgaatttctc caatacgctt
     6181 ccaagaaatc aattttaact aaatataaaa ttcccgctca gtcggtcgaa ggctatcttt
     6241 tccccgatac ttatttttta ccgcctggat attccaaaga gaagattatt gaacacatga
     6301 tcgatcactt cttcgataaa gtcaaaacgg taaaaaattt ccccaccgac ccgcgtaaaa
     6361 tacacgagac agttatttta tcttcgattg ttgaacgcga agccaagctt aaagaagagc
     6421 gcgccctcat cgccgccgtg tttcacaacc ggctcaaaga cggcgagccg ctcgaatcgt
     6481 gtgcgacggt gcagtacctt tttgaaaaac caaaggcgcg cc
//
LOCUS       DAOMPD010000090         6520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5062_length_6520_cov_2.610054, whole genome shotgun sequence.
ACCESSION   DAOMPD010000090 DAOMPD010000000
VERSION     DAOMPD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_6520_cov_2.610054"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..712
                     /locus_tag="PLY93_04420"
     CDS             308..712
                     /locus_tag="PLY93_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRP68754.1"
                     /translation="MAKTPFSVKELNEFKELLSEKKARLIKEIQGKEDELTSKDKDEV
                     GDMADMATELIERELKMSLSEGDRERLEAIDAALDRIKTKSYGICIDTGEVINKARLK
                     AVPEALRTLVAQEAHDKQQRKRKTLSRTVEVE"
     gene            713..1435
                     /locus_tag="PLY93_04425"
     CDS             713..1435
                     /locus_tag="PLY93_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68755.1"
                     /translation="MRVDLYPELSNVLADVARLLRNRDFFLWRREDEPLPESISLSFK
                     PVVSEKVIERAVNKNACTLCARRLSYKKGQFEEPTITKPYLFLVHNTFLGPRANFYND
                     AAENTLFEKMIMGVLGFNASDALVREILRCHFATDETGTVEQVRNCETHVRHDIETYH
                     IKGIILFGQAAAFVYRDKNELLALQDKVFTWQNLPTMVCPGPNRLQYMRVKKYPKEQI
                     EAERIKIFETLKIFKEKVIELV"
     gene            complement(1508..1753)
                     /locus_tag="PLY93_04430"
     CDS             complement(1508..1753)
                     /locus_tag="PLY93_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68756.1"
                     /translation="MLKKLLALLLALTFVGGVVAQDAETTPAEASTEAPAKQEGKKHK
                     KAKKAKKKKAKKAKKKKRKAKKAEAPAEPAAADESGN"
     gene            2083..2325
                     /locus_tag="PLY93_04435"
                     /pseudo
     CDS             2083..2325
                     /locus_tag="PLY93_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            2643..4391
                     /gene="mutL"
                     /locus_tag="PLY93_04440"
     CDS             2643..4391
                     /gene="mutL"
                     /locus_tag="PLY93_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRP68757.1"
                     /translation="MKRIQKLNETLIAQIAAGEVIESPAAIIKELVENSLDANATEID
                     IRVVGDGFSEIQIRDNGDGIHANDLFLAVDSFATSKITSFDELTNTTTMGFRGEALGS
                     IAAVSRLTIESKVRMATQGASVHVDETGRHLVPSPIREGTRVVVRDLFYNVPVRRDYY
                     QNRPKVLRQITDTLTQITIAHPTITFRYAIDNKEPVYLPASQNLIERLTTIFGESIRE
                     NLIPLYYEENGLTLEGYISKFYFYRNHAGDIRLWVNKRPVQYKPLVMLLRHVYGELMP
                     KGRFPFAALFFSLAPREVDANVHPQKREVRFKQEARVQNFLRQAFVRTIGQEGGISAA
                     SMVAVSGGGGSPTLEKIPQRIASEKPATPVILLEHDSPFKASQLFDFQPEISTSAFPE
                     NLTLHTRIFNTFIVATNPEGIFLIDQHTAHERINYEHFQDLLAEKKDISQRLLHSIPI
                     MNAVFTRSQFSLYKKTLLSLGFETTDLGPAGFALTAVPGYVDTGEELDAFETALHIVN
                     GAEGVETADLFDQLAKDLSCRHAIRKGETASLADFREQIEKLKLCRMPLRCPHGRPTV
                     IRIDEKEIFAYFKRQV"
     gene            complement(4375..>4867)
                     /locus_tag="PLY93_04445"
     CDS             complement(4375..>4867)
                     /locus_tag="PLY93_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRP68758.1"
                     /translation="NLTVRTKPHSRKHPRPVIVMQTTRPIKTGLRTLANKAIGGELWI
                     SQATPNTTLQEVWPELNEDWPIYSFSRVTEIVASLKERGFNKILLEGGPTLNGFFFEA
                     ALVDEFFLTILPILWGGTTTDRTVITPNPLPITHFRLQSAERRKNEMFFHYKKRTKTI
                     PAA"
     assembly_gap    4868..4877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4878..5073)
                     /locus_tag="PLY93_04450"
     CDS             complement(<4878..5073)
                     /locus_tag="PLY93_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRP68759.1"
                     /translation="MPQKISAPPKTKTTIRLNMAMTIDGHVAHPTQAWEFGSAEDRRR
                     MDRLREWADCLIVARRTIEHD"
     gene            complement(5079..5588)
                     /locus_tag="PLY93_04455"
     CDS             complement(5079..5588)
                     /locus_tag="PLY93_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68760.1"
                     /translation="MIDVGEEIRIQKKSGPTSLFLHIPYLNYVFFIFQGRGAYKIIKN
                     LYEYKKVVPGFFQSAWLTIKFSLLSTIASTALFSLLLAMGYNPFGKVLAKYIYALSTL
                     IGGLLPASQLALLFGEVPNSLLFPTFTLPTFIALGIITFGTYFVFGMAGAFLAIFNIK
                     ADIRRAQFY"
     gene            complement(5620..>6520)
                     /locus_tag="PLY93_04460"
     CDS             complement(5620..>6520)
                     /locus_tag="PLY93_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68761.1"
                     /translation="SLLLRPRQTASQWFDDVASKVTLALFTLIVLAIPSFFLILQNKY
                     LLLYYIAYPTTMGLALYLRTLKKECLSSIVTIIGVYGVVVWLAVLVDIKSISYLVWWI
                     ISLLPWVLFTEKQRVWLLLTAALPILFSFLSTSIPLPQSPILPAEQDFLRQMLHISVA
                     LGAFSCIYFLKSALNQSEKQRTLENELYNLCFNSIPLPVIIKDAITLEYIYFNTAAQM
                     TFGLSQDRLYSNAILFSESCATHISLLDRDTLKTMAYHIESEERLEYINSEIYWRFRT
                     YRIPLLLQESRRRILLTVYELRD"
BASE COUNT         1832 a         1335 c         1550 g         1793 t
ORIGIN      
        1 atatatatta taaataccag aaaacgctta atttttctca ctttttgaaa aaaaaagtca
       61 aaaaatactg attttttgaa aaaaattgcc tttttgtaca cgctacgcga gtttacgcac
      121 aggctcttag gagtacccta ttattagtta ataagtgtta tgtcacatat cacgatattt
      181 tcttgcactg gataccttaa atttgtcatg ttaccacagt cgatttagct ctaactcttg
      241 cacataaatt tcatcttgct tgtcaaaaaa aatggccaaa tcaataagaa tgagttaaat
      301 tgcgcgaatg gcaaagactc cattttcagt taaagagctg aacgaattca aagagcttct
      361 ctcagaaaaa aaagctcggc ttatcaaaga gattcaaggg aaagaagatg aattaacttc
      421 aaaagacaaa gatgaagtgg gcgacatggc cgatatggca accgaattga ttgaacgtga
      481 gcttaaaatg tctctctccg aaggggatcg cgaacgtttg gaagcaatcg atgcggcact
      541 cgatcgcatc aaaacaaaat cttatggtat ttgcattgac actggtgaag ttatcaataa
      601 agcacgactc aaagccgtac ccgaagcgtt gcgtacttta gtggcgcaag aagcgcacga
      661 taaacagcag cgtaaaagaa agacattatc gcgtaccgtt gaagttgagt aattgcgcgt
      721 cgatctttat ccagagctga gtaacgttct tgccgatgtg gcgcgtttgt taagaaatag
      781 agatttcttt ctctggcggc gagaggacga acctctgcct gaaagtattt cactttcttt
      841 taagcccgtc gtctctgaaa aagtgattga aagagcggtg aataaaaatg cgtgcacgct
      901 ctgtgcgcgt aggctttcgt acaaaaaagg acagtttgaa gaacctacaa taacaaaacc
      961 ttatttattc ttggttcata atactttttt ggggccacgc gccaattttt ataacgatgc
     1021 tgcagaaaat acattattcg aaaaaatgat aatgggtgtg cttggtttta atgcaagtga
     1081 tgccttggta cgtgaaatct tacgctgcca ctttgctaca gacgaaacgg ggacggtcga
     1141 gcaagtgcga aattgtgaaa ctcatgttcg acacgatata gaaacttatc acatcaaagg
     1201 gattattctt tttgggcaag cggcggcgtt tgtgtatcgc gataagaacg aattattagc
     1261 gctgcaagac aaagtattta cttggcaaaa tctaccgaca atggtttgtc ctgggccaaa
     1321 tcgacttcaa tatatgcggg ttaaaaaata tccgaaagag cagatagaag cagagcgaat
     1381 aaagattttt gaaacgttaa agatttttaa agaaaaagtt atagagttag tttgataatt
     1441 tgcttggggc tgcctaaaca ccgtaaaaac aatcgataga cagcccaagc aatgcagtta
     1501 aagtggctta gtttccgctt tcgtctgctg ctgcaggttc tgcaggcgct tcggctttct
     1561 tggctttacg ctttttcttt tttgcttttt ttgccttttt ctttttggct tttttcgctt
     1621 ttttgtgttt tttgccttcc tgttttgcag gtgcttctgt agatgcttcc gccggtgtag
     1681 tttcagcgtc ttgtgctact acgccaccta cgaaagtaag tgccagtaat aatgctaata
     1741 attttttcag catttggttc tcctaaattt cttaatcttt gcgttccaag gcttttgacc
     1801 acgggtgcgt ggaacacccg tgttagcttt ttcccgcgtc aataataata cgttgacaag
     1861 gccgagaaac ggacaaaaat ggttatggtt gctaatatat cgctaacggt atttttgaca
     1921 atcttatttt cagagtgtca gtatggttcg acagttcgaa agccttcaaa gacgtaggtc
     1981 aagggtgatt tattctcttg gtcgtgttgt aaataggcat ggaaaccatg tctttttcat
     2041 cggcgcactg agaggataaa cttcaggaga aaagtaacaa gaatgaatat ctatgtaggt
     2101 aatttggcgt ataacgccac agacgaagaa ttgcgcacag cctttgaagc attcggtaca
     2161 gtgactttag tgaaaattgt acgcgatcgc gactcaggca gatcacgcgg ctttgcattt
     2221 gtcgaaatgg aagatggtga aggcgcacaa aatgcaattt cgcaactgaa tggacgcgat
     2281 atgaaaggcc gcaatctcgt tgtgaacgaa gcacgccctc gcgaagaagg taaccgtggt
     2341 ggtcacggcg gcggtggcgg tcgaagtggc tatggcggcg gcggtggtcg tggcggtagc
     2401 cgcggtggcc gtggcggcgg cggcaacttt cgtcgtggtc atagcgacga aaacgcgagg
     2461 tagaactgtc gaataaccac taattaggct ggctaacagg cgcattagcg acttattaga
     2521 cagccctgat agacggtcag tgtttttgtt tgcatttttg ggtattcgta ctcaaaaatg
     2581 caaacattcc ccaataagtg tcgttaaagc gccctatata aaggcataca gttttcatta
     2641 atatgaaacg tattcaaaaa ctcaacgaga cactcattgc acaaattgcc gccggtgaag
     2701 tcattgaatc gcccgccgct atcattaaag agttggttga gaactctctc gatgcaaacg
     2761 cgactgaaat cgatatccgt gttgtcggcg atggattttc agaaattcaa atccgcgaca
     2821 atggcgacgg tattcacgcc aatgatttat tccttgcagt ggatagtttt gcgacgagta
     2881 aaattacttc ttttgacgaa ttaacaaaca caacgacaat gggctttcga ggcgaagcgt
     2941 tgggctccat cgccgcagtg agccgcttga cgatagaatc taaagtgcgc atggcaacgc
     3001 agggggcttc ggttcatgtc gatgaaacag gcagacacct tgtcccctca ccgattcggg
     3061 aaggtacgcg cgtcgtggta cgcgatctct tttacaacgt accggtgcgt cgggattatt
     3121 atcaaaaccg tcccaaagtt ttgcggcaaa ttaccgatac actgactcaa atcacgattg
     3181 cgcaccctac aattactttc cggtatgcga tagacaataa agaaccggtg tatctccctg
     3241 cctctcaaaa tttaattgaa cggttgacta cgatttttgg cgagagcatt cgtgaaaatt
     3301 taattcctct ttactatgaa gaaaacgggt tgaccctcga agggtatata tcaaagtttt
     3361 atttttatag aaaccatgca ggcgatatcc gcttatgggt gaataagcgt ccggtgcagt
     3421 ataaaccgct cgtgatgctc ttgaggcatg tgtatggtga actcatgccc aagggccgtt
     3481 ttcctttcgc cgccttattt ttttcccttg cgccgcgcga ggtcgatgcg aatgtgcatc
     3541 cacaaaaacg tgaagtgcgt tttaagcaag aagcgcgcgt acaaaatttt ttgagacaag
     3601 catttgtgcg taccatcggc caagaaggtg gtatttctgc ggcgagtatg gtggctgttt
     3661 caggtggtgg ggggagtcct actttggaga aaataccgca aaggattgct tcagaaaagc
     3721 cagcaacacc ggttatcctt cttgaacacg acagtccttt taaggcgtct caactttttg
     3781 attttcaacc ggagatatcg acgagcgctt tcccggaaaa tcttacctta cacacgcgta
     3841 tttttaatac ctttattgtc gccacaaacc ccgaaggtat ttttcttatc gaccagcaca
     3901 ccgcgcacga acggattaat tacgaacatt tccaagattt gctggctgaa aaaaaagata
     3961 tttcgcagcg tttactccac agcataccca tcatgaacgc tgtttttact cgaagccagt
     4021 tttcattata taaaaaaact ttgttgtcgt tggggtttga gacaaccgat ttaggacctg
     4081 ccggttttgc cctaacggcc gtccctggct acgttgacac gggcgaagaa cttgatgcgt
     4141 ttgagactgc gctccacatt gtgaatggcg cagagggagt ggaaactgcc gatttatttg
     4201 atcaactcgc taaagattta tcgtgtcggc acgcgatccg caaaggcgag actgcttcgc
     4261 tcgccgattt tcgggagcaa attgaaaagt taaaattatg ccgcatgccg ctgcgctgtc
     4321 cgcatggcag gccaacagtc attcgtatcg acgaaaaaga aatctttgct tattttaagc
     4381 ggcaggtata gtttttgtgc gctttttata gtgaaaaaac atttcatttt ttcgccgctc
     4441 ggcgctttgt aaacgaaagt gcgtgatagg tagcgggtta ggggttatca cggtgcgatc
     4501 ggtggttgtc cctccccaga gaatgggtag aattgtcaga aaaaactcat ctacaagcgc
     4561 cgcttcaaaa aaaaagccat tgagcgttgg tccgccttcc aagagaattt tattaaaccc
     4621 gcgctcttta agcgaggcga caatttcggt cacgcgcgaa aatgaataaa tgggccagtc
     4681 ttcattgagc tctggccata cttcttgcag tgtggtgtta ggtgtcgctt gggaaatcca
     4741 tagctcgccg ccaatggctt tattggccaa tgtccgtaaa ccggttttga tggggcgcgt
     4801 tgtttgcatg acgatgaccg gtcgggggtg tttgcggctg tgcggttttg tgcgcacagt
     4861 gagattcnnn nnnnnnncgt cgtgttcgat ggtacgccgc gcaacgatga ggcaatccgc
     4921 ccactcgcgt aaacgatcca tccgccgcct gtcctccgcc gagccgaatt cccacgcttg
     4981 ggtggggtgt gcgacgtggc cgtctatcgt catcgccatg ttgaggcgga ttgtggtctt
     5041 tgttttaggc ggggcgctta ttttttgtgg cacaattttt aatagaattg tgcacggcgg
     5101 atatcggctt tgatgttaaa aatcgccaag aatgcccctg ccataccaaa aacaaagtag
     5161 gtaccaaaag tgataatgcc taatgcgata aacgtgggca aagtaaacgt cgggaaaagt
     5221 aaactgttgg ggacttcccc gaaaagtaga gcaagctgcg aagcgggcaa aagaccgccg
     5281 atgagggtgc tgagcgcata gatatacttt gcgagcacct tcccaaacgg gttatacccc
     5341 atcgccaaaa gtaggctaaa aagcgccgtc gatgcaattg tgcttaaaag agaaaatttg
     5401 attgtgagcc acgccgattg aaaaaagccg gggactactt ttttatattc gtataaattt
     5461 ttaatgattt tatacgcgcc tcgtccttgg aaaataaaaa aaacataatt caagtacggt
     5521 atatgcaaaa acaggcttgt ggggcctgac tttttttgaa tacgtatttc ttcaccaaca
     5581 tcaatcatag tggattgacc tctgtacgaa atggtattgt caatctctta attcatatac
     5641 tgttaagaga atacgccgcc gactttcttg taataaaagt gggatgcgat aagtgcgaaa
     5701 ccgccagtaa atttctgagt ttatgtactc caaacgctct tcactctcga tatgatacgc
     5761 catcgtttta agagtgtcgc ggtctaaaag agaaatatga gtggcgcacg actcgctaaa
     5821 aagaatggcg ttagagtata gtctgtcctg cgatagccca aaagtcattt gcgctgcggt
     5881 attaaagtaa atatattcga gtgtaatggc gtctttgatg ataacaggta gaggaatgga
     5941 attaaaacag agattataga gttcgttttc gagagttctt tgtttttccg attgattgag
     6001 cgccgattta agaaaataaa tgcagctaaa cgctcccaac gctacggaaa tatggagcat
     6061 ttgtcttaaa aaatcctgtt cggcgggtag aatggggctt tggggcagag gaatcgatgt
     6121 acttaaaaaa gagaatagaa tagggagcgc agcggtaagg agtaaccaaa cccgctgttt
     6181 ttctgtaaaa agcacccacg gcaataaaga aataatccac cagacgagat acgatatact
     6241 tttgatgtcg actaataccg ccaaccaaac cacgacaccg tacacgccga tgattgtgac
     6301 gatgctactt aaacattctt tcttcagagt gcgtagataa agcgctaatc ccatcgtcgt
     6361 tgggtatgcg atgtagtaga gaagcaagta cttgttttgc agtattaaaa aaaaggaggg
     6421 tattgctaga acgatgagag taaaaagtgc aagcgttact ttgctcgcaa catcatcgaa
     6481 ccattgcgat gccgtctgcc tagggcgaag gagaagagag
//
LOCUS       DAOMPD010000091         6508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5072_length_6508_cov_2.493259, whole genome shotgun sequence.
ACCESSION   DAOMPD010000091 DAOMPD010000000
VERSION     DAOMPD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_6508_cov_2.493259"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="xseA"
                     /locus_tag="PLY93_04465"
     CDS             <1..1059
                     /gene="xseA"
                     /locus_tag="PLY93_04465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRP68762.1"
                     /translation="PLAFELKVGIEVELFGSLGTYAPQSSYNLVVRRVQKVGAGDILL
                     KLQQIRERLLKDGLIKTPQTRRALPLLPKRVGIVTGAGTAALRDMLLQVQERYAHAEI
                     VLAPAMVQGDAAPASIVAALTEIQKIEWQCEVIIVGRGGGSAEDLMAFSDEAVCRAVA
                     TCRVPIVSAVGHQIDHPISDDVADMAATTPTAAAQAVFPKIDDFSSTLDNLSKRIASS
                     VDGALRTIQERFLRTSESGFWDEPYLLVKDQARFLDELESKLHFTFRNRVMTYSNALN
                     QLAPIEILLAQKLKLTRAAFETTSARFDAYSPLATLARGYSVVYKEDKIITDSAHLKR
                     GDTIRVRLAKGSVAAAVE"
     gene            1068..2606
                     /locus_tag="PLY93_04470"
     CDS             1068..2606
                     /locus_tag="PLY93_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HRP68763.1"
                     /translation="MKESSSTSEQRSTEKNPPLHRAWILLALMVTMMLAAMDTTIIST
                     AIPQIVEDLGGFAYFSWVYSIYLLAQTVTIPIYGKLADLYGRKPVLLFGTSLFLVGSA
                     ACASAWNMASLIAFRGLQGLGAGSILATVNTLAGDIFTVEERAKIQGWLSSVWGMAAI
                     LGPSLGGAFVEYASWQWIFLINLPIGIVSLLLIAIFLHESFEKRTHTIDYAGAFFILV
                     TGVLLLVSLMQIGNAWPLFSYQSMGLIFALVVLFLLTLRIESGASEPIMPTWVWKNKT
                     LIAANLAMVCMGIIMIGPNMYIPIFSQSVLRLDAIASGFVLASMSLGWPLASSLSGKL
                     YLKIDFRNTAMIGTVLIFAASGGFLLLPFDAPAWLLIFDQALMGAGFGLLSTPTLVGV
                     QSIVSWSERGVVTGANMFSRYLGQSIGAAILGGFFNSAMRRELAISPTSLQEQLPHKI
                     NDVVGVLKNSSSTQAVTDYLRHAFYLASHHVFSVMAFVAVLIFLFLCLMPRKLKIVAA
                     AQGS"
     gene            2653..3555
                     /locus_tag="PLY93_04475"
     CDS             2653..3555
                     /locus_tag="PLY93_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRP68764.1"
                     /translation="MRAIKPIFIILILLIGNGCAEKPQVQTGKAGCPTLPPDGMECIP
                     GGFYKLGTNRTDFKKELYELSAFAEHTVELSPFFIDRYEVTTEEYQRCVVAKKCNAVR
                     SNYPQMRGAKQPQLKANWFEATAYCKTQGKRLPTEAEFEAASRGPQGEDYPWGNEPAN
                     CTRAVIKDEHGRGCPHMKDAKGFVPTPERFKDAGNTWEVGSRPIGRYGLYDMAGNAQE
                     WVSDWFVDSLEKCGSACTEKNPKGPCAGKEKCTVSKQKLVKGGAWYWGQMSALAAVRR
                     PYFPENDPPHHFGFRCVKDASVKN"
     gene            complement(3557..4396)
                     /locus_tag="PLY93_04480"
     CDS             complement(3557..4396)
                     /locus_tag="PLY93_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRP68765.1"
                     /translation="MLRRFHALFIVALTLPFFSCALLKNSLSEYAPTISLKDVLLKGF
                     DFEGADLEFKYTIQNRAAFGITFNKLAFQILVDGKRMVDAQNDKNVAIKAKETTEFSI
                     IQRVRYVETATAFLDLVKKDSVTIALKGVVGVYINELVGSVSVPIEASKVVPVPKLPS
                     IAFGSFDFGSMNMANPLNPSATFNLKFKINNANPFDIKVPKVEYAFTAAGTNVVSGKK
                     QNQNLAKSSTAQLAIPVTLRGREIIDLVPKLRDLSSTDYKFDSAVEFNVMGQPLRVPF
                     SYP"
     gene            complement(4497..5888)
                     /locus_tag="PLY93_04485"
     CDS             complement(4497..5888)
                     /locus_tag="PLY93_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68766.1"
                     /translation="MQKRTLIIALTIVFTMIGCAFQNGDDDIIGSKRADNVRDLSLIE
                     ARNTIAPTTSTVQFDKTKEAVKEVQVGDVIVSDVNGKSPNGFLKKVVNVVENAQDKII
                     VTEETSITEAVEEGQASAEGILNPAGDNSLLTGATYNQFNGQIETITPEPGVTVYGTK
                     KPGTLPPEIPSNCSQAKAKRWYVEFDKTIQSSGTIKGCVGMDLAFKLNMKIKWFKLKS
                     AEFSLAPSLITEIKTNLNGNTFDINRAQVTLTTFNLQPITFTIGFVPVVIIPRIKIVL
                     GVDGKVTATLVSTFQFEASAKAGIVYADSKWSVIKERSSKYNFNTPVFTGNINAMVYA
                     GPEVTLLFYGIVGPGANLYAYTHFNVDWDAGLNKPKRWDIWLGVEAGAHFTVEMFGRT
                     LFKINEPSILGYEWLVASSDFWGIAPPGTGNVTGVLGSRLNGTYTKSDNTSSTMSGDL
                     ITVGNLVGTLAKK"
     gene            5993..>6508
                     /locus_tag="PLY93_04490"
     CDS             5993..>6508
                     /locus_tag="PLY93_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRP68767.1"
                     /translation="MAHKNKAKRVHAFGNDVLGERDATALAHLIKRREVSALEVAKAA
                     VERAERVNTALHAVELPTYEQALRDAVNPPAAGIFAGVPTFIKDNADVSGLPTRTGTR
                     ATKGHTAKKHGDFTAQFIRQGFTVLGKTTMPEFGFNASTEFLLDEPTHNPWNTDFSSG
                     GSSGGSAAMVAA"
BASE COUNT         1549 a         1579 c         1671 g         1709 t
ORIGIN      
        1 ccgcttgcct ttgagctcaa agtgggtata gaagtcgagc tctttggttc gttggggact
       61 tatgcaccgc aatcatcgta caatttagtc gtgcggcggg tgcaaaaagt cggcgcgggg
      121 gatatactcc ttaaattaca acaaattcgc gagcgcttac ttaaagacgg tctcattaaa
      181 acaccacaga cgcgccgcgc gttgccgcta ctccccaagc gtgtgggtat cgtcaccggt
      241 gcgggcaccg ccgcgctgcg cgatatgctc ttgcaagtcc aagaacggta cgcgcacgct
      301 gaaattgtgc ttgcgccggc gatggtgcaa ggcgacgccg cccccgcatc gattgtggcc
      361 gccctcactg aaattcaaaa aatcgaatgg cagtgcgagg tcattattgt ggggcgtggc
      421 ggtggttccg ccgaagatct catggcgttt agcgacgaag cggtgtgccg cgccgtggcc
      481 acttgtcgtg tgccgattgt ctcggcggtg gggcaccaaa tcgaccatcc catttccgac
      541 gacgtggccg acatggctgc caccacaccc acagcagcgg ctcaagcagt ttttcctaaa
      601 atcgacgatt tttcgagcac gttggataac ctaagtaaac gcattgcaag cagtgtcgac
      661 ggcgctttgc gcacgatcca agagcgcttt ttacgcacct ctgaaagcgg tttttgggac
      721 gagccgtatc ttctcgtcaa agatcaagcg cgttttttgg atgaactcga atcaaagctg
      781 cattttacgt ttaggaacag ggtgatgacc tattccaatg cgctcaacca gttggcgccg
      841 attgaaatcc tactcgcgca aaaattaaaa ctgacgcgtg ccgcttttga aacgaccagc
      901 gcccgctttg atgcgtattc gccgctggcg actctcgcgc gtggatactc ggtagtttat
      961 aaggaagata aaattatcac cgacagtgca cacctaaaac gaggcgacac gatacgcgtg
     1021 cgtttggcaa aaggaagcgt agcggcggcg gtggaatgag aaggattatg aaagagtctt
     1081 catctacaag cgagcaacgc agtaccgaaa aaaatccccc tttgcaccgt gcgtggattc
     1141 ttttggctct catggtcacc atgatgctcg ccgcaatgga cacaacgatt atctcgacgg
     1201 cgatacctca aatcgtcgaa gacttgggcg ggtttgctta tttcagttgg gtgtactcga
     1261 tttatctttt agcgcaaacc gtaacaattc ccatttatgg taaactcgcc gatctttatg
     1321 gccgcaaacc cgttttactt tttgggacat cgcttttttt ggtagggtcg gcggcgtgcg
     1381 ccagtgcatg gaatatggcg tcgctcattg cgtttagagg tttgcagggg ttgggcgcgg
     1441 gttccatttt agcaacggtc aatactttag cgggcgacat tttcaccgtc gaagagcgcg
     1501 ctaaaatcca aggctggctt tcgagcgtgt ggggcatggc ggcaatcttg ggaccgtctt
     1561 taggcggtgc atttgtggag tacgcgtcgt ggcaatggat ttttttaatc aatttaccca
     1621 tcggcattgt gtctcttctc cttattgcaa tttttctcca cgaaagtttt gaaaagcgta
     1681 cgcatacgat tgattacgcg ggtgcatttt ttatccttgt gacgggtgta ttgctgttgg
     1741 tgtcgctcat gcaaattggt aacgcctggc cacttttttc ataccaaagc atggggctta
     1801 tttttgcgct cgtggttttg tttttgctga cgctccgcat tgaaagcggc gcaagcgaac
     1861 ccattatgcc tacatgggta tggaaaaaca aaacactcat cgcggcgaat ttggcgatgg
     1921 tgtgtatggg tatcatcatg attggcccca acatgtatat ccctattttt tcgcaatcgg
     1981 tgttgcgttt agacgccatc gcttcgggtt tcgttttggc gagcatgagc ttgggctggc
     2041 cgcttgcatc gtcgttgtcg ggtaagctat acctcaaaat tgattttcgt aacacggcga
     2101 tgatcggcac cgtgcttatt tttgccgcct cgggtggatt tcttcttttg ccttttgacg
     2161 cacctgcgtg gttacttatc ttcgaccaag cgttgatggg cgctggcttc ggtcttctat
     2221 caacccccac tttggttggc gtgcagtcga tcgtaagctg gagcgagcgc ggtgtcgtca
     2281 cgggagcgaa catgttctct cgttatttag gccaaagcat cggtgcggct attttgggtg
     2341 gattttttaa ctcagccatg cggcgtgagc tggccatttc tcccacaagc ttacaagagc
     2401 aactcccgca caaaataaac gatgtggttg gtgtgttaaa aaatagcagt agcacacaag
     2461 cagttaccga ttatttgagg cacgcttttt atcttgcttc acaccacgtt tttagcgtga
     2521 tggcttttgt cgccgtcctc atttttctct tcctgtgtct catgccgcgt aagctcaaaa
     2581 tcgtcgcagc ggcgcagggg agttaagtga aactatttat ttacgcacgc atagagagat
     2641 tacggcttgt ctatgcgtgc aataaagcca attttcataa ttcttatact ccttatcggc
     2701 aacgggtgcg ccgaaaaacc acaagtgcaa acaggaaaag cgggatgccc cactttacca
     2761 cctgacggca tggagtgcat tccgggtggt ttctacaaat tgggaacaaa ccgtaccgac
     2821 ttcaaaaaag aactctacga actttcggca ttcgcagaac acacggtcga gttatcgcca
     2881 ttttttatcg atagatacga agtcaccacc gaggaatacc agcgctgtgt ggttgcaaaa
     2941 aaatgcaacg cggtgcgctc gaactatcca cagatgcgcg gcgcaaagca accgcaactg
     3001 aaagcaaatt ggtttgaggc tacggcgtat tgcaaaacac aggggaaacg ccttcccacc
     3061 gaagcggaat ttgaagccgc gagccgtggt ccccaaggtg aggattatcc gtgggggaat
     3121 gagcccgcca attgcacccg tgcagttatt aaagacgagc atggccgtgg ttgcccccac
     3181 atgaaagacg caaaaggctt cgtccccact cccgaacgtt tcaaagatgc gggcaatacc
     3241 tgggaagtgg gttcgcgccc tataggccgc tatggtcttt atgatatggc aggcaatgcg
     3301 caagagtggg tgagcgactg gtttgtcgat tcgctcgaaa aatgcggtag cgcgtgtacc
     3361 gaaaaaaatc cgaaagggcc ctgcgcgggg aaagagaaat gcaccgtatc aaaacaaaaa
     3421 cttgtcaaag gcggtgcgtg gtattgggga cagatgtcag cgcttgcggc agtacggcgt
     3481 ccctactttc ccgagaatga cccaccccac cactttggtt tccgctgcgt gaaagacgcc
     3541 tctgtgaaaa actaacttaa ggatagctaa acggcactcg cagtggttgt cccatcacgt
     3601 tgaactcaac tgccgaatcg aacttgtaat cggtcgagct taaatcacgc agcttaggca
     3661 cgaggtcgat aatttcgcgg ccacgcaatg tcacggggat ggccaactgt gcggttgaag
     3721 atttagcgag gttttggttt tgcttcttac cgctcaccac attggtgcca gcggcggtga
     3781 acgcgtattc cactttaggg actttgatat cgaacgggtt agcgttattt attttaaatt
     3841 ttaagttgaa cgtcgcgctg ggatttagcg ggtttgccat attcatgctg ccaaaatcaa
     3901 acgaaccgaa tgcaatcgac ggtagcttgg gcacaggcac cactttactc gcttcgatgg
     3961 gcacactcac cgagccgacc aattcgttga tataaacacc aacgacacct ttgagcgcta
     4021 tggtgacgga atcttttttt acaaggtcca aaaatgcggt cgcggtttca acatagcgca
     4081 cgcgttggat aatggaaaat tcggtggttt ctttcgcttt aatcgccacg ttcttgtcat
     4141 tttgcgcgtc gaccatgcgc ttaccatcga ctaatatttg aaacgcaagt ttgttgaagg
     4201 tgatgccaaa cgctgcacgg ttttggattg tgtatttgaa ttctaaatca gctccttcaa
     4261 aatcaaagcc tttgagcagc acgtctttga gagaaatcgt gggggcgtat tcgctgaggc
     4321 tatttttaag gagagcacag gaaaagaaag ggagggtgag cgccacaata aagagcgcat
     4381 ggaagcgacg aagcatagtt aatccatgaa tacggttgcg tcgtgtcaac cgtattcact
     4441 tcttctattt attcagactt gtttaagaac agcgagttcc agtctgacca cctttactat
     4501 ttcttcgcga gcgtaccgac taaattaccg acggtgatga gatcgcctga cattgtcgag
     4561 gacgtgttat cacttttcgt atacgttccg ttcaatcggc tgcccaagac gccagtgaca
     4621 ttaccggtgc cgggaggcgc aatcccccag aaatcggatg aagccacgag ccactcatac
     4681 cccaaaatcg acggttcgtt aatcttgaat agagtacgtc cgaacatctc gacagtaaaa
     4741 tgcgctcctg cctcgacacc taaccaaata tcccagcgtt tgggtttatt gagccctgcg
     4801 tcccaatcga cgttgaagtg ggtgtacgcg taaaggtttg cccccgggcc gacaatgccg
     4861 tagaagagaa gggtcacttc agggcctgcg tacaccatcg cgttgatatt gcctgtgaac
     4921 acgggggtgt taaaattgta cttactgctt ctctctttaa tgaccgacca tttgctgtct
     4981 gcataaacaa tccctgcttt tgcacttgct tcaaactgga aggtgctcac gagagttgcc
     5041 gtgactttgc cgtcgacacc caagactatc ttgatgcggg gaataatcac tacgggcaca
     5101 aagccaatcg tgaaagtgat cggctgtaaa ttgaatgtgg tgagggtcac ttgagcacgg
     5161 ttgatatcaa acgtgttacc gttcaagttg gttttaattt cggtgatgag cgaaggcgca
     5221 agtgaaaatt ctgcactttt gagtttgaac catttaattt tcatattgag cttaaacgct
     5281 aaatccatac cgacgcagcc tttaatcgtg cccgaacttt gaattgtttt atcaaactca
     5341 acataccatc ttttagcttt tgcctggctg caattcgaag gaatttctgg aggcaaggtt
     5401 ccgggttttt tggtgccgta gacagtaacc cctggttcgg gagtgatggt ttcaatttgt
     5461 ccgttgaact gattgtatgt cgcaccggtc aaaagagaat tatcgccggc agggtttaat
     5521 ataccctccg cggatgcttg accttcttca accgcttcgg tgatcgaagt ttcttcagtt
     5581 acaataattt tatcttgcgc attttcgaca acattaacaa cttttttcaa gaagccgttg
     5641 ggagatttac cgttcacgtc ggaaacaata acgtcgccca cttgaacttc tttcaccgct
     5701 tctttagttt tgtcgaattg aacggtgctc gttgtgggag caatcgtgtt ccgcgcttcg
     5761 atgagcgaca agtcgcgcac gttgtcggcg cgtttgctac cgataatgtc gtcgtcgcca
     5821 ttttggaatg cacaaccgat cattgtgaat acaatggtca gtgctatgat aagtgtcctt
     5881 ttttgcataa tttctccctt taaggttact tgatatatat gccttattta gggtcgtcga
     5941 tgccaatttt tttcatgggt tgacgcggta attcaaccta cgggcataac agatggctca
     6001 taaaaacaaa gcaaaacgtg tacatgcgtt cggcaacgat gtcttgggcg aacgcgacgc
     6061 gaccgcattg gcgcatctca tcaaacggcg tgaagtttca gccctcgaag tggccaaagc
     6121 ggcggttgaa cgcgccgaac gggttaatac ggcgctacac gcagtggagt tgcccacgta
     6181 cgagcaagcg ttgcgcgacg cggtaaatcc ccctgctgcc ggtatctttg cgggggttcc
     6241 tacctttata aaagataacg ccgatgtgtc gggactcccc acccgcactg gcacgcgcgc
     6301 gaccaaaggc cacactgcga aaaaacatgg ggatttcacc gcacagttta ttcggcaagg
     6361 gttcaccgtg ttgggaaaaa cgacgatgcc cgaatttggt tttaacgctt caacggaatt
     6421 tttactcgac gagcccacgc acaacccgtg gaacaccgat ttctcttcgg gcggttcgtc
     6481 gggcggctcg gcagcgatgg tcgcagcg
//
LOCUS       DAOMPD010000092         6474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5104_length_6474_cov_2.520642, whole genome shotgun sequence.
ACCESSION   DAOMPD010000092 DAOMPD010000000
VERSION     DAOMPD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_6474_cov_2.520642"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="dnaA"
                     /locus_tag="PLY93_04495"
     CDS             <1..1000
                     /gene="dnaA"
                     /locus_tag="PLY93_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRP68768.1"
                     /translation="DRFIKGPSNEHAYAAAIAAAQKPNNYHNPLYIYGGVGLGKTHLM
                     MAIGNYVAEAYPWMKIKYVPSEIFQSDLIDAMRDKNLPYFRTRYRSVDVFLLDDVQFI
                     SKNADFTQEEIFHTFNYFYQNKKQIVISADRPPQALPQLHDRLQTRFQQGLIVDVKSP
                     NFETRMAIFKTKGHEAGIQIPPDVLKYLATRFTSQVRSMESAINTLLFTSRHEKRPID
                     LQMAKICLKALPQESGSNQITIEDILRVVSRTLHVDEALILGANRTEQVALARHVSMY
                     LAKKLIQGATLSYIANAFGKRDHTTVMHAEQKVRELIDTDAALQMQIDEMLEELKF"
     gene            complement(997..2250)
                     /locus_tag="PLY93_04500"
     CDS             complement(997..2250)
                     /locus_tag="PLY93_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68769.1"
                     /translation="MRSIVIWVGAVVFVIGAGVFFIEREYISSDKNARIRLEKGKLQL
                     ERFDEDGLRSGIDELTTVVAQYPDSVYARQARYHLAEAYERLGMRDVALGKYKKLLTM
                     PLTDELKAQVKFKVAKLQVLRNYSDEGMNQLLHLLNTTADKNLRSEIYLEIAKYYHRK
                     DLKKDALRNFQNALTENPESREARAALGLGDADYQNSVALHGILDKTGSSQTQEQKPD
                     AVKPATTEKEKQVVVNPQFAAKLAEGIQAFSRSDYRNAVRLLTPIANSTAKESEDALY
                     YLGNAYLKLKEYRNAVHFLNKAVSNTYRERDEAAFIKKGEALYLNNQFERALKVFNFV
                     RTHYTKGKYAQIAADWEGETKRMLGEKYGDEKSIPMANDDLVDDDDDLWESSSNEDAL
                     SPQPKYNSRRDDRREEITLDDDVTP"
     gene            complement(2252..3163)
                     /locus_tag="PLY93_04505"
     CDS             complement(2252..3163)
                     /locus_tag="PLY93_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68770.1"
                     /translation="MRRLQRVMLKFVTTLFLVCVASVSPVVAQYLNPDSTFRDLPPEK
                     KPAVEAPPQISPPQTPPPNPVVHATREEQFVDFAAYVREKNIILTWHFLTGRTNDLRV
                     QIYRFTEEPKVIHDISSGVLVAKLTGDINLYEDVPPQKGAYYYAVFVETQRGLEPASF
                     TPARNLVGPLYFRAAETEKNPSASVPVQTVKPASQEIPTAAFEIEEPEVERESIDAVD
                     TRRLNMIIRKTYLKGKYREAIRYLKPYLRHKMPKIRAKAIFYSGLARYRLGEGERALK
                     YFEHSLTQKYYRKNADFWINRIMENSR"
     gene            3226..4014
                     /locus_tag="PLY93_04510"
     CDS             3226..4014
                     /locus_tag="PLY93_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRP68771.1"
                     /translation="MISYALVKIISFNVNGIRAIEKKGELQKLIDTEKPDLLFVQEIK
                     AKPEQLSDALLNPTGYEAHYHSAEKPGYSGTGVLIKEKTFSKTTILRGMPEWNDHEGR
                     VLGVKADDLIVFGNYFPNGGKSDAAWNEKLVFYDHFHKYINSLRKKNRVLFTGDLNVA
                     HNEIDLARPKENEKHVGFRPEERAWMNRLTADAWVDVFRTRYPTTVSYTWWQMQSRAR
                     ERNIGWRIDYFVVDKPLYAAVKDMRHLTDHQGSDHCPIVLEIKL"
     gene            4018..5247
                     /locus_tag="PLY93_04515"
     CDS             4018..5247
                     /locus_tag="PLY93_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP68772.1"
                     /translation="MKNQKAFDALSLPGYRLFLLTFMLTMMADNIEHVISYYIIFQKF
                     KSPALAGFAVISHWVPYLFFSVYVGALNDRFDSRRLIQIGGTLFAIVSAGWGYFFLTD
                     TLEMHHAMALLALHGLSGVFWMTSSQVLLYDIVGQEHLASAVRLMSTVRYLAVFIGPG
                     VGSVLMLTAGATWGMFINTAFYLPLLIWLLRAPYGRALRRIADGRKRAVKGIIDILQT
                     FRDVRPIRILTTMIFITGAASFFIGNGYQAQMPEFAADLGNGNPGTAYSMLLAADAAG
                     ALIAGILLESRGSIFELKVTNVFILAIGWAFALLGFSLMRSYYFAIMLLFCAGFFELS
                     FSSMAQTLVQMNAPDAIRGRVLGLYNMVGSGLRLFSGITVGLVGSLMTIHASLAFAAT
                     GFIVVCGVLLFYIKRVH"
     gene            5463..>6474
                     /locus_tag="PLY93_04520"
     CDS             5463..>6474
                     /locus_tag="PLY93_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRP68773.1"
                     /translation="MLALRSLVLVLLIQLPLLAATLSGVVTTPDKNPIAGANILLPTL
                     RLHEHSNADGTFSIPNLSAGNYTVRVHKSGFPPQSVEVHLSEPGKHIEIELALYSIRT
                     KKVVISGFRPTDEADFSQSATVIEGKKLDRERGQNLLDSVRDIPGVVAESTGNAVAKP
                     VMRGLSAYRSLLLLDGVPEEAQQFGDEHGPNMDNLDMARIEIIRGPQSLIFGAGAMGG
                     AMNIVTPPLPSSAEGSERLSAKLIGDILSNNPGGAGALSLSGAAGKIGYRGSFSYRET
                     GDTRTPNSIVPNSNYKNWNGSTLIGIQEKWGFLSLRASHYDTTIHLPEATTDASGRLI
                     ADI"
BASE COUNT         1640 a         1646 c         1444 g         1744 t
ORIGIN      
        1 cgaccgcttc attaaaggcc cttcaaatga gcatgcgtat gcagcggcaa tagcagcagc
       61 gcaaaagccc aataactacc acaaccctct ttatatttat ggcggtgtag gtctaggtaa
      121 gacgcactta atgatggcaa ttggtaacta tgtcgccgaa gcgtatcctt ggatgaaaat
      181 caaatatgtg ccatctgaaa tttttcaaag cgacctcatc gatgcaatgc gggataaaaa
      241 tctcccttat tttcgtaccc gctaccgcag cgtcgatgtc ttcctgttag acgacgtaca
      301 atttatcagc aaaaatgccg atttcacaca agaagaaatc tttcacactt ttaattattt
      361 ttaccaaaat aaaaaacaaa tcgttatctc cgccgatcga cctcctcaag cgctgcctca
      421 actacacgac cgattacaga ctcgattcca acaagggctt atcgtcgatg taaaaagtcc
      481 caactttgaa acacggatgg caattttcaa aaccaaaggc cacgaagcag gtatacaaat
      541 tccccccgat gtactcaaat accttgcaac ccgcttcact tcgcaagtgc gctcaatgga
      601 atcggcgatt aacacactac tctttacttc acgccatgaa aaacgcccca tcgatttgca
      661 gatggccaaa atatgcctca aggccttacc gcaagaaagc ggatcaaatc aaatcacaat
      721 tgaagatatt ttacgcgtgg tgtcgcgcac gctacacgtc gacgaagcgc tcattttagg
      781 ggcaaaccgc accgagcaag tcgccctcgc ccgtcacgtt tcgatgtact tggccaaaaa
      841 gctcatccaa ggcgcaacac tcagctacat cgcgaatgct tttggaaagc gcgatcacac
      901 gacggtgatg cacgccgaac agaaagtacg cgaactcatc gataccgacg ccgcgctaca
      961 aatgcaaatc gacgaaatgc tcgaagaact caaattctaa ggcgtgacgt cgtcgtcgag
     1021 agtaatttct tcacgacgat cgtctctgcg actattgtat ttaggttgcg gtgaaagagc
     1081 gtcctcattc gatgaacttt cccataaatc atcgtcgtca tcaactagat catcgttggc
     1141 catagggata gatttctcat cgccgtattt ttcacccaac atgcgctttg tctcgccttc
     1201 ccaatcggca gcaatttgag catacttacc tttcgtataa tgcgtacgca caaaattaaa
     1261 cactttgagc gcacgttcga attgattgtt taaatacaac gcttcgcctt ttttaatgaa
     1321 cgccgcttcg tcgcgctcgc ggtaggtatt gctcaccgct ttattcaaaa agtgtacggc
     1381 gttgcggtac tcttttaatt tcaaataagc attgcctaaa tagtaaagcg cgtcttcgct
     1441 ttctttggcg gtagaattgg caatcggcgt tagcaaacgc accgcgttac ggtagtcgct
     1501 tcttgaaaaa gcttggattc cctctgcaag ttttgcggca aactgcggat tgacgacgac
     1561 ctgtttttct ttttctgtag ttgctggttt taccgcgtcc ggtttttgct cttgagtttg
     1621 gcttgaaccg gttttatcca atataccgtg tagcgcgact gagttttggt aatcggcatc
     1681 gcccaacccc aacgcggcgc gcgcttctct gctttcagga ttttcagtca acgcgttttg
     1741 aaaattacgc aacgcatctt ttttaagatc tttgcggtga taatatttag caatttctaa
     1801 ataaatttcc gagcgtaaat ttttatccgc agtcgtgttg agtaaatgca acaattgatt
     1861 cataccctcg tccgagtaat ttctgagaac ttgcaatttt gcaactttga atttcacttg
     1921 cgctttgagt tcatcggtaa gcggcatggt taacaatttt ttatacttac ccaatgccac
     1981 atcgcgcatt cccaagcgtt cgtacgcttc ggccaaatga taacgcgcct gccttgcgta
     2041 cacgctatcg ggatattgcg ctaccactgt ggtgagttcg tcaatcccac tgcgcaaacc
     2101 gtcctcgtca aaacgctcta attgcagttt gcctttttct aaacgaatgc gcgcgttttt
     2161 atcgcttgaa atatattccc gctcgataaa aaaaactccc gcaccgatga caaagacaac
     2221 cgccccaacc catattacaa tactacgcat attacctcga attttccatt atacgattaa
     2281 tccaaaaatc cgcattcttg cggtaatatt tttgtgtaag cgaatgctca aaatacttca
     2341 gcgcgcgttc tccctcgcct aaacgatagc gcgcaaggcc gctataaaaa attgcctttg
     2401 cccggatttt aggcatcttg tgtcgcaggt atggtttcaa gtaacgaatc gcttctcgat
     2461 acttgccctt gagatacgtt ttgcgaataa tcatatttaa ccgtcgtgta tcaacggcgt
     2521 cgatactctc acgctcgact tcaggttctt caatctcgaa tgccgctgtt ggtatttctt
     2581 gcgacgccgg tttcaccgtt tgcacgggca cacttgcaga tggatttttt tccgtttcag
     2641 cggcacgaaa ataaagcggc cccactaaat tacgcgcggg agtaaacgat gcaggttcca
     2701 agccgcgctg cgtttcgacg aacactgcgt aatagtacgc ccctttttgc ggcggtacat
     2761 cttcgtataa attaatatca ccggtgagtt ttgccaccaa aacccccgaa gagatgtcgt
     2821 gaatcacttt cggttcttcg gtgaagcggt aaatttgcac gcgcaaatca ttcgtacgtc
     2881 ctgttaaaaa atgccaagtg agtataatat ttttttcccg cacatacgcc gcaaagtcga
     2941 caaactgttc ttcgcgtgtc gcatgtacca ccgggttggg aggcggcgtt tgaggagggg
     3001 atatttgagg cggagcctct acggcgggct ttttttccgg cggtaaatcg cggaatgtgc
     3061 tatcggggtt gaggtattgt gcaacgacag gcgataccga tgcaacacat actaaaaata
     3121 aggtagtaac aaatttaagc atcactcttt gcagccgccg catagctctc tcttatgtgt
     3181 cggcacgttt atacactaaa gtttatttat tttcggcctc tttcaatgat tagttatgca
     3241 cttgtgaaaa ttatctcgtt caacgtgaac ggtatccgcg ctattgaaaa aaaaggagag
     3301 ctccaaaagc tcatcgatac tgaaaagccc gacttactct tcgttcaaga aatcaaagca
     3361 aagccggaac agctctctga tgctttactc aatcccactg gttacgaggc gcactaccac
     3421 agcgcagaaa agcctggtta ctcaggcact ggagttctga ttaaagaaaa aactttttca
     3481 aaaacaacta ttttgcgcgg tatgccagag tggaacgacc acgaaggaag ggtgctggga
     3541 gttaaagctg atgatctcat tgtgttcggc aattattttc ccaacggcgg gaaatccgac
     3601 gccgcgtgga acgaaaaact tgttttctac gatcactttc acaaatacat caattcacta
     3661 cgtaaaaaaa accgtgtgct tttcaccggt gacttaaacg tagctcacaa cgaaatcgac
     3721 ctggcacgcc cgaaagagaa cgaaaaacac gtgggctttc gccccgaaga gcgtgcgtgg
     3781 atgaaccgcc ttaccgcaga cgcttgggtc gacgtgttcc gcacccgcta ccccaccaca
     3841 gtgagttata cgtggtggca aatgcagtcg cgcgcgcgcg agcgaaacat cggttggaga
     3901 atcgattatt ttgtcgtcga taaaccactc tacgctgcgg ttaaagatat gcgccatctc
     3961 accgaccacc agggctcaga ccactgtccg attgtgcttg aaattaaact ctaacaaatg
     4021 aaaaatcaaa aggcattcga cgcactgtcg ttgccgggat accggctatt tctactcacc
     4081 ttcatgctca ccatgatggc cgataacatc gaacacgtaa ttagctatta cattattttt
     4141 caaaaattta aatcccctgc acttgccggg tttgctgtca tctcgcactg ggtaccatat
     4201 ctttttttct cggtgtacgt aggtgccttg aacgaccgtt tcgattcgcg tcgcctcatc
     4261 caaatcggcg gcaccctctt tgctatcgtc tccgcgggct ggggttattt cttcttaacc
     4321 gacacgttgg aaatgcacca cgcaatggcg ctacttgcgc tacacggcct ttcaggtgtg
     4381 ttttggatga cttcgagcca agtgttactc tatgacatcg tcggccaaga acatctcgcc
     4441 agcgcagtgc gcctcatgtc caccgtacgc tatctggcag tttttatagg gccaggtgtc
     4501 gggagtgtat tgatgctcac cgcaggagca acgtggggga tgttcatcaa caccgcattt
     4561 tacttgccgc tcctcatttg gctcctgcgc gcaccttatg gacgcgcgct tcgtcgaatt
     4621 gccgatgggc gcaaacgtgc agtcaaaggg attatcgata ttctacaaac cttccgcgat
     4681 gtgcgcccca tccgcatact cacgacgatg attttcatca ccggcgctgc ttcatttttc
     4741 atcggcaacg gctaccaagc gcaaatgccc gaatttgccg ccgacttagg aaacgggaat
     4801 cctggcacgg cgtattcgat gcttttggcc gccgacgctg cgggcgcact catcgctggt
     4861 attttactcg aaagccgagg gtctatcttt gaactcaaag tcaccaacgt ttttattttg
     4921 gccatcggct gggcttttgc tttattagga ttttcactca tgcgctctta ttattttgca
     4981 attatgcttc ttttttgtgc aggctttttc gagctatcgt tcagcagcat ggcgcaaacc
     5041 ttggtgcaaa tgaacgcccc cgacgccatt cgtgggcggg tgttggggct ttacaatatg
     5101 gtgggctcgg gcttacgcct tttcagtggt atcacggtgg gactcgttgg tagtcttatg
     5161 accatccacg cgtcgttggc gtttgcagcg acaggcttca ttgtggtttg cggtgtactg
     5221 ttgttttata taaagagagt tcattgatag tattatgtca cattaggggt aactatccag
     5281 cattatgtca cattagaggg taactatcca cacgaatgct ggtgctcaaa aataaggaaa
     5341 aatttaaagg aaaacaatct ttcaaaccct ctgactactg cttatcatcc acctccaaca
     5401 gatattgata aatcattatc atttatgctt gacgcactac ttggattctt atccgatgag
     5461 tcatgttagc gttgcgttca cttgtactcg tcttgcttat tcaacttcct ctacttgctg
     5521 cgaccctgtc aggtgtggtc acgacccctg acaaaaaccc gatagctgga gcaaacattc
     5581 tcctccccac actacggctg cacgaacaca gcaacgcaga cgggaccttt tccataccga
     5641 atctctctgc cggtaattac acagttcgtg tgcacaagtc aggctttcct ccgcagagcg
     5701 tagaagtcca tttgtccgaa ccaggtaagc acattgagat tgagttggcg ctctattcca
     5761 tcagaacgaa aaaggtcgtc atctccggtt ttcgtcccac cgacgaagcc gatttcagcc
     5821 aaagcgcaac ggttattgaa ggcaaaaagc tcgaccgcga gcgtggtcaa aatttactcg
     5881 atagtgtccg cgatattccc ggagtcgttg cagaaagtac ggggaatgcc gtagcaaaac
     5941 ccgtgatgcg tgggttgtcg gcgtatcgct cgttgttatt actcgacggc gtaccggagg
     6001 aagcgcaaca atttggcgat gagcatggcc ccaatatgga taacctcgat atggcccgta
     6061 ttgaaattat acgcgggcct caaagtctca tcttcggtgc gggtgcaatg gggggtgcga
     6121 tgaatattgt cacccctcct ctgccgtcga gcgctgaagg gagcgagcgt ctctcggcaa
     6181 aactcatagg agatatttta tcgaataatc ctgggggcgc aggcgcgctc tcgctctcgg
     6241 gcgcagcggg taaaataggt taccgaggtt cgtttagtta tcgtgaaacg ggcgacactc
     6301 gcacgcccaa tagcatcgtt cccaacagca attataaaaa ctggaacggt agtacgctta
     6361 tcggcattca agaaaaatgg ggatttcttt cgttacgtgc gtcgcattat gacacgacga
     6421 ttcatttacc cgaggcgacg accgacgcat cggggcgcct cattgctgac atcg
//
LOCUS       DAOMPD010000093         6446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5137_length_6446_cov_3.493819, whole genome shotgun sequence.
ACCESSION   DAOMPD010000093 DAOMPD010000000
VERSION     DAOMPD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5137_length_6446_cov_3.493819"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PLY93_04525"
     CDS             complement(<1..1548)
                     /locus_tag="PLY93_04525"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HRP68774.1"
                     /translation="MTQQDQKELGRTLWAIADQLRGAMNADDFRDYMLSFLFLRYLSD
                     NYEAAAKKELGKDYPDNNAKATATPLQIWYEKNASDVVDFEKQMRRKVHYVIKPDFHW
                     ASVAELARRQDKELLHTLQKGFKYIETESFESTFQGLFSEINLDSEKLGKNYTERNAK
                     LCTIITKIAEGIAQFSTSVDILGDAYEYLIGQFAAGSGKKAGEFYTPQQVSDILSKIV
                     VLDGQEPKSGPKKKLEKVLDFACGSGSLLLNVRKQLGANGIGKIYGQEKNITTYNLAR
                     MNMLLHGVKDSEFEIHHGDSLLNDWEILNEMNPAKKLEFDAVVANPPFSYRWEPTDAL
                     GEDFRFKNYGLAPKSAADFAFLLHGFHFLGKEGTMAIILPHGVLFRGGAEERIRRKLL
                     EDGNIDTVIGLPANLFFSTGIPVCILVLKKCKKPDDVLFINAAEHFEKDKRQNRLTDE
                     HIEKIVETYQHRREEPRYSRRVTMEDIATNEFNLNISRYISTAKAEEEIDLKAVNGRL
                     VELNAKIT"
     gene            complement(1545..2162)
                     /locus_tag="PLY93_04530"
     CDS             complement(1545..2162)
                     /locus_tag="PLY93_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68775.1"
                     /translation="MERFKNKYRIATTRLQNWDYRWAGAYFITVCTQNRLHYFGEIKN
                     GQMQLSPIGVIADVLWHEIKNHTKNVELGEFIVMPNHIYGVLIIIDNNDTINADAHVE
                     TGHTLSLRGDIALSLRGRVRGLQTIGQQRFQNIGKNSISSIVGSYKSAVTKHAHRLGF
                     DFVWQRLFHDRIIRNENSLHRITNYIRLNPENWQKDTYFKIEEPP"
     gene            complement(2314..3639)
                     /locus_tag="PLY93_04535"
     CDS             complement(2314..3639)
                     /locus_tag="PLY93_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRP68776.1"
                     /translation="MAKQKNKSPKTFPSSGGVAEGRGGHVPRLRFPEFRSQDSWPALT
                     MNEISTRITEKVGSEKLTPVSITAGKGFVTQTEKFGRDISGDQYKNYVLLREGEFAYN
                     KGNSKKYAQGCIYKLKEFKKVAAPYAYISFRLNDGYEPDFFQFYFEMNAHGKQLQKYI
                     TSGARSDGLLNIDPIDFFRISLPIPSLPEQQRIADCLTSLDEVISAESQKLDALKVHK
                     KGLMQQLFPSEGGTSTSSVATVPRLRFPEFRDSGGWEEKTLGKIAEIKRGAGSQYLEY
                     VEDSQLGIRLLRINDFLNNDAVYVRNTEDMKRFRVKAGDLLIAGTGATAGITYMVPKE
                     YDNLAYSYNAPRIRLTEGNNYFIAQYLKSDHIFEQQTRLFVGNAQPFLDTNAIANFKI
                     CLPSLAEQKRIANCLSSLDDLIAAQAEKIDALKEQKKGLMQGLFPVAGE"
     gene            complement(3645..4667)
                     /locus_tag="PLY93_04540"
     CDS             complement(3645..4667)
                     /locus_tag="PLY93_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRP68777.1"
                     /translation="MSKKRENKIDSQDRKKSFDEILQHIQQARQKVFTQINTALIDLY
                     WFVGKTISQKVQSEAWGKGVVAEFAEYIAQKAPEIKGFSDKNLWRMKQFYETYQADQK
                     LAPLVRELPWTQNTLIFSRCKTTEERAYYLRLCINERYTSRELERQIDAAQYERQKLN
                     PPKLSATLRELHPGIHQTFKDNYVLEFLGLPQAHTENDLQKALLAHMKTFILELGKDF
                     IFIAEEFRLQVGNQDFSIDLLFFHRGLSALVAFELKIGKFSPEHMGQINFYLEALDRD
                     VKKPHENPSIGVLLCRDKDDEVVEYALSRNLSPTLIAQYRLQLPDKKLLRARLHELLP
                     QDWDNS"
     gene            complement(4842..>5145)
                     /locus_tag="PLY93_04545"
     CDS             complement(4842..>5145)
                     /locus_tag="PLY93_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HRP68778.1"
                     /translation="AQWRVPDNRPLADFAPTIILKAKDFATEITIHNARTNQMKSESE
                     ISYEHVTNNKAVRATLLNRGIRPESLPPAEDVKKVERRLDAAAEKTIKKPDKLAGQ"
     assembly_gap    5146..5188
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5189..5728)
                     /gene="dinD"
                     /locus_tag="PLY93_04550"
     CDS             complement(<5189..5728)
                     /gene="dinD"
                     /locus_tag="PLY93_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HRP68779.1"
                     /translation="MENEIIYTLTETFEGHAQATENGVEYWLARDLQHLLGYTKWDNF
                     LNVVSKAKTACEVSGHEVPDHFADVGKMVDLGSGSKREISDIMLTRYACYLIAQNGDP
                     AKQEIAFAQTYFALQTRKAELIQQHLLEAERVSARKKLSATEKELSEVIFEQTSGNQN
                     FALIRSKGDQALFGKSTQAM"
     gene            complement(5747..>6446)
                     /locus_tag="PLY93_04555"
     CDS             complement(5747..>6446)
                     /locus_tag="PLY93_04555"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRP68780.1"
                     /translation="LKGRQARGAVDFDTVETQIVCDEAGRIEKIVPPDELRAFRGAYL
                     ETAQRLRAEQKKSGDEVVQQLDFEFVLFASAIIDYDFIMGLIARFTHEKTEKHKMTRT
                     ELIGLIASDAKFIDEREDIEAYIDTLKVGQSRTEQEIRQGYQKFKEEKFNQGLAALAT
                     AHSIDLTALTTFVQAIRDRMVFDAQALSDLFAPLELGWKARAQKELAFMEDFIPLLKK
                     LAQGREISGLGAYE"
BASE COUNT         1478 a         1505 c         1499 g         1921 t
ORIGIN      
        1 ggtaatcttg gcattgagct caactagccg gccatttacc gccttcagat caatttcttc
       61 ttctgccttt gccgtactaa tgtaacgcga aatattgagg ttgaattcat tcgttgcaat
      121 atcttccatc gtgacccgac gggaatagcg cggctcttcg cgccgatgtt ggtaagtttc
      181 aacaatcttc tcgatatgct cgtcggtgag tctattttgg cgcttatctt tttcaaagtg
      241 ttcggccgca ttgatgaata acacatcatc tggcttcttg cactttttca acacgagaat
      301 gcaaacggga ataccagtcg aaaaaaacag gttcgccggc aggccgataa ccgtatctat
      361 attaccgtct tcgagcagct tgcggcgaat acgctcttcg gcgccgccac ggaatagcac
      421 accgtgcggc agaatgatcg ccattgtgcc ttctttgccc agaaaatgga agccgtgcag
      481 cagaaaggca aaatcggcgg cagatttggg cgccaggcca tagttcttga aacggaaatc
      541 ctcacccagc gcgtcggtcg gttcccagcg gtagctgaat ggcggattcg cgacgacagc
      601 gtcaaactcc agttttttag ctgggttcat ctcgttcaga atttcccaat cattgagcag
      661 cgagtcgccg tggtgtattt cgaactctga atctttcaca ccgtgcagca gcatattcat
      721 gcgcgcgagg ttgtaggtgg tgatattttt ttcctgaccg tagattttgc cgataccgtt
      781 ggcgccgagt tgcttgcgta cgttcagcaa caaagaaccc gaaccgcagg caaagtccag
      841 gactttctca agtttcttct tggggcccga tttcggctcc tggccgtcga gaacgacgat
      901 cttggataga atatcagata cctgttgtgg cgtgtaaaac tccccggctt ttttacccga
      961 accggcggca aactggccga ttaggtattc gtaggcatcg ccgagtatgt caacgctcgt
     1021 cgaaaattgg gcaatgccct cggcgatctt ggtgatgatg gtgcacagtt tggcgttgcg
     1081 ctcggtataa ttcttgccca gcttctctga atcgagatta atttctgaaa atagaccctg
     1141 aaacgtgctt tcgaaagatt cggtttcgat atacttgaag cctttttgca gggtgtgcaa
     1201 caattcctta tcttgtctgc gagccagctc ggcgacactg gcccaatgga agtcgggttt
     1261 gataacgtag tgcaccttgc ggcgcatttg cttttcaaag tcgaccacat cgctggcatt
     1321 tttttcatac cagatttgca gcggcgtcgc ggtcgcctta gcgttattgt caggataatc
     1381 tttcccgagt tctttctttg cggcagcttc gtagttatca gacaggtagc gcaagaacag
     1441 aaacgacagc atgtagtcgc gaaagtcgtc ggcgttcatc gcgccgcgca gttggtcggc
     1501 aatggcccag agggtgcggc cgagttcttt ttggtcttgt tgggtcatgg tggttcttct
     1561 atcttaaaat aggtatcttt ttgccaattt tctgggttta gtctaatgta atttgttata
     1621 cgatgcaatg aattttcatt acgaataatg cggtcgtgaa aaaggcgttg ccatacaaaa
     1681 tcaaaaccta accggtgggc gtgtttggta accgcagatt tgtacgaccc gacaatggat
     1741 gatatggaat ttttaccgat attttgaaac cgttgttgcc cgatggtttg taacccccgt
     1801 acccgccccc gtagagacag ggcaatatcc ccacgtagag acagggtatg ccctgtctct
     1861 acgtgggcgt cggcgttgat ggtgtcatta ttatcgataa taatcaaaac cccgtagata
     1921 tgattgggca tgacaataaa ttcacccaat tctacatttt ttgtatgatt ttttatttcg
     1981 tgccacaata catcggcaat aacgcctatg ggtgataatt gcatttgtcc gtttttaatt
     2041 tcgccaaaat aatgcaaacg gttttgggta cataccgtaa taaaatatgc gcctgcccaa
     2101 cggtaatccc aattttgcaa tcgtgttgtt gcgatacggt atttattttt gaatcgttcc
     2161 attattgatt attggtttgt cgtcgcccac cgcgacgtaa cgacgtaacg acgtagagac
     2221 agggcatgcc ctgtctctac gtcgtcgtgg taggccgtgg tgcgtcgtgg tacgaaacga
     2281 tattgataat tttttgcgtg ccaatcgcat attttattca cccgccaccg gaaacaaccc
     2341 ctgcatcaac cccttcttct gctctttgag cgcgtcaatc ttctctgcct gcgcggcgat
     2401 gaggtcgtcg agggaggaga ggcagttggc gatgcgtttt tgttcggcga gggagggtag
     2461 acaaatcttg aaattcgcta tcgcattcgt atccagaaat ggttgggcat tcccaacaaa
     2521 aagccttgtt tgttgttcaa agatatgatc tgattttagg tattgggcaa taaagtaatt
     2581 attcccttct gtgagtctga ttcgcggagc attgtaacta tatgccagat tgtcatattc
     2641 ttttggaacc atgtaagtaa tgccagctgt tgctcctgtt cctgcgatta ataaatcgcc
     2701 ggccttaact ctaaatcgtt tcatgtcttc cgtgttccga acgtaaacag catcgttatt
     2761 caagaaatca tttatcctaa gtaagcgtat gcctaattgc gagtcttcaa catattctaa
     2821 atattgtgaa ccagcccctc tttttatctc tgcgattttt cctaaggttt tttcctccca
     2881 ccccccacta tccctaaact ctggaaaccg cagccggggc acggtggcca ctgagcttgt
     2941 cgaagtgcca ccctctgacg gaaaaagctg ctgcatcaac cccttcttgt gtaccttgag
     3001 cgcgtcgagc ttctggcttt cggcgctgat aacttcgtcg agcgaggtga ggcagtcggc
     3061 gatgcgttgt tgttcgggga gggaggggat ggggagactt attcgaaaaa aatcaatcgg
     3121 atcaatattg agcaatccat cagaacgagc accgctagta atatattttt gaagttgctt
     3181 gccatgagca ttcatttcga agtaaaattg aaagaaatcg ggttcatagc cgtcgttcag
     3241 acgaaaagag atgtaggcat aaggtgctgc aactttctta aattccttta atttgtaaat
     3301 gcagccctgc gcatattttt ttgaatttcc tttgttgtaa gcgaattcac cctcgcgcaa
     3361 tagaacataa ttcttatatt gatcacccga aatatcacgt ccgaacttct ctgtctgtgt
     3421 aacgaaacct ttgccagccg taatgctgac tggtgtcagt ttttcactcc caaccttttc
     3481 ggttatgcgc gtgctgattt cattcatggt cagggctggc cagctgtctt gagaccgaaa
     3541 ctcgggaaac cgcaggcgag gcacgtgacc accccggcct tcggccaccc ctccagagga
     3601 ggggaatgtt tttggggatt tatttttctg ttttgccata tcttttatga attatcccaa
     3661 tcttgcggca acagttcatg caaccttgcc cgaagcagtt ttttatccgg tagttgcagt
     3721 cgatattggg cgatcaacgt aggcgaaagg ttgcgagaca aagcatattc gacaacttcg
     3781 tcatctttat cccgacataa taatacgccg atactgggat tttcatgtgg ttttttgacg
     3841 tcgcgatcga gtgcttcaag ataaaagtta atctggccca tatgttcggg tgaaaattta
     3901 cctatcttca attcaaaagc aaccagtgcg gataatccgc gatgaaagaa cagcagatct
     3961 attgaaaaat cctgattgcc gacttggaga cgaaactcct cggctataaa aatgaagtct
     4021 ttacctaatt ccagaataaa ggttttcatg tgagcaagaa gggctttttg caagtcgttt
     4081 tcagtatgtg cttgtggcag gcctaagaat tccaatacgt aattgtcttt gaatgtttgg
     4141 tgaataccgg gatgcaattc tctcagcgtt gccgagagtt ttggcggatt cagtttttgg
     4201 cgctcatatt gcgcagcgtc gatttggcgc tccagttcac gcgatgtgta tcgctcgttg
     4261 atacaaagtc taagataata tgcacgttct tctgttgttt tacagcgcga aaaaataaga
     4321 gtgttctgag tccacggcaa ttctctcacc agtggtgcga gtttttgatc ggcttggtac
     4381 gtttcataaa actgtttcat gcgccacagg tttttatcgc tgaaaccctt tatctcgggg
     4441 gctttttgag caatatattc tgcaaattca gcaaccacgc ccttacccca tgcttcactt
     4501 tgcacttttt ggctgatagt tttgccgacg aaccaataca agtcgattaa ggcagtattg
     4561 atttgcgtaa atactttttg ccgtgcttgt tgaatatgtt gtaaaatttc atcaaacgac
     4621 tttttcctgt cttgagaatc tattttattt tcacgcttct tactcatttc aatagtcttt
     4681 tatttcgtga aagcgtttag accagcttct tttctaaccc cctcgaattc gaggggggta
     4741 aaaagagata tgaaatcgtt ttcagcaatc gatataattg ttatctctgt attgcgtaca
     4801 tttattttag tcatagccag tccttgcctt tcatgcctgt cttactgccc tgccagtttg
     4861 tcgggcttct tgatcgtctt ttctgctgcg gcatcgaggc ggcgctctac cttctttaca
     4921 tcttcagccg gcggtaaact ttccggcctg ataccgcgat tcagtagcgt cgcgcgcaca
     4981 gccttattgt tcgtcacatg ctcgtaggat atttcagact cacttttcat ttggtttgtt
     5041 cgggcattgt ggatggtgat ttcggtcgca aaatcttttg ctttgagtat aatggttggt
     5101 gcgaaatcag ccaaagggcg attatctggt acgcgccatt gagctnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tggcttgcgt cgatttgcca aataatgcct
     5221 gatcgccttt gctgcgaatc aatgcaaaat tttgatttcc actggtttgc tcgaaaataa
     5281 cctcagaaag ttctttctcg gttgccgaaa gtttcttgcg tgccgaaact ctttcggctt
     5341 ccagcagatg ttgctgaatc aattctgctt tgcgtgtttg cagagcgaaa taagtctgtg
     5401 caaaagcgat ttcttgtttg gcggggtcgc cgttctgggc aatcaggtaa caggcatatc
     5461 gagtgagcat gatatcgctg atttcacgct tactgccaga gcctaaatcg accattttcc
     5521 cgacgtcggc aaaatggtct ggaacctcgt gtcctgagac ttcacaagcc gttttggctt
     5581 ttgaaacgac gttcagaaag ttgtcccatt ttgtataacc cagcaaatgc tgtaaatccc
     5641 gggcgagcca gtattctacg ccattttccg tagcctgggc atgaccttca aaggtctcgg
     5701 ttaaagtgta gatgatttca ttttccatat ttataccgct gctttactat tcgtacgctc
     5761 ccaagcctga aatctcgcgc ccttgcgcca gtttcttgag caagggtatg aagtcttcca
     5821 tgaaggccag ttctttttgt gcacgagctt tccaaccgag ttccagtggg gcaaaaaggt
     5881 cactcaatgc ctgcgcgtca aaaaccatac ggtctctgat cgcctgcacg aaggtggtca
     5941 acgccgtcag gtcgatgcta tgcgccgtag caagagctgc cagcccttga ttaaatttct
     6001 cctctttgaa tttctgatag ccttggcgga tttcctgctc ggtgcggctt tggcccactt
     6061 tgagagtatc aatataagct tcaatgtctt cgcgctcgtc gatgaattta gcatctgagg
     6121 caataagtcc aatcagctca gtgcgcgtca tcttgtgctt ttcggtcttt tcgtgcgtaa
     6181 agcgcgcaat gagtcccatg ataaaatcat agtcgatgat cgccgaagca aacagtacga
     6241 actcgaaatc gagctgctgc acaacttcat cgccgctttt cttctgctcg gccctgaggc
     6301 gttgtgcggt ttcgaggtat gccccgcgaa atgcgcgcaa ctcgtctggc ggcacgatct
     6361 tctcgatgcg gccggcctcg tcgcagacga tctgcgtttc gacggtgtcg aagtccaccg
     6421 cgccgcgtgc ctggcgccct ttcagc
//
LOCUS       DAOMPD010000094         6430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5157_length_6430_cov_2.983529, whole genome shotgun sequence.
ACCESSION   DAOMPD010000094 DAOMPD010000000
VERSION     DAOMPD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5157_length_6430_cov_2.983529"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..2058
                     /locus_tag="PLY93_04560"
     CDS             121..2058
                     /locus_tag="PLY93_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP68781.1"
                     /translation="MNVAQLKVNTYVEAIEQSFKNNGQRLALRYRDGSEDFKTMTYSE
                     LEAEVRVIAAGLAAIGVKPKNHVGLIADVGHYWLMSNLAIQLNGAADVPRGTDSTGPE
                     LAYIVDHSGADFVLVYNGEQVKKIEAGLKKHKHRVKKYIVLNSDHTAKGANVMTLNDL
                     KKEGEALIKKGGKTLAEFESRAGKVTASDLSCLVYTSGTTGEPKGVMLTHGNFASQMR
                     IMPGVLGAGNEDSGLTLLPPWHVFGRITEMMFVAIGASVTYTDIKHIGEDLRAVKPTY
                     VPAVPRIWEGVYNKIIGNVKKQGKEPIFNKFKTAALKFNHYSAMLTGKEKRPIKRSFF
                     SAIVARLVGLLGVLLWLLPKKLGDILVFKKVVAATGGNLKASISGGGALPSYIDDFFR
                     AIGVRILEGYGLTETSPVLSVRQPSRIIPGTVGPLIAETEYKLIDLEGNDVTKIPGAK
                     GTLHVRGPQVMQGYYKNPKKTAEVLTPDGWFNTGDLVIINHSGEISIVGRSKDTLVLV
                     GGENVEPTPIEEKMKESPFIDHVMLVSRAFGEDQKVLGALIAPNLDNLGEWCKANNVA
                     GNATSWLKDAKVNALYRAEINKYINAENGFKNFEKVGVFALLPKPFEQGDELNNTLKV
                     KRHVVNDKYHKLIDEMYKAAK"
     gene            2106..>2726
                     /locus_tag="PLY93_04565"
     CDS             2106..>2726
                     /locus_tag="PLY93_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRP68782.1"
                     /translation="MLLSIIIDRVGNTNIFNIIQEGEVSNPRLKVHERLQSIIDDDLI
                     AEYLEELGRIANISRSLSSLPKGSEENQALIFQHLNLVDKLREIGSALFNQFFPTPLQ
                     EYIRNTDPNFLYFHVDAALASLPLEIFHDGTAFLWEKFYLGKAVKGQDTTFSELNPRE
                     TINMLIIADPSENLEWARREGELLFEHLGANVNPKKIHLTLIGGKSV"
     assembly_gap    2727..2760
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2761..3997
                     /locus_tag="PLY93_04570"
     CDS             <2761..3997
                     /locus_tag="PLY93_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRP68783.1"
                     /translation="LNAILDKDIIHYAGHLHYHESAGENGWVLADGKIFYAREFKKSG
                     AQPKLIFCNSCLSARSDQHATDSSWYAHFASAFIRAGRTSYIGTNWELPDRKPTLEFT
                     THFYDNVFKGKPLGEALQHSRVYAREHFSLNDLTWASYILMGNPQQTVFRSESLLPDV
                     THNILDPDVVSTQYPYPIADAFKKFHSITTQTEGSAEQATATLPALFTLFERSVFFLS
                     ALILANYRIFNFPKGIAFPYPDVQKCLTGLFSALGTIHAIKALPLATNLLETLYVHKE
                     NLFKLANLETRFRRENLKKSDYETYSITVQYHIEALLTDLDFLRHYGFYFIVEPGHRQ
                     LSFHGTEENHTHRDILLPTQTNSVVYTEILEKTSLLVGRCVFYSPVKKMFLDLSPFMQ
                     INADAEGTYTFNFTKAKGE"
     gene            complement(3975..4553)
                     /locus_tag="PLY93_04575"
     CDS             complement(3975..4553)
                     /locus_tag="PLY93_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68784.1"
                     /translation="MQTWLCVAHEKEIHNLKKFTRVGFTPYALGVGQFFALAEFSRLV
                     TLEKPKQVVLVGTCGSLYKEDIFQLYHATQFTFPSIEGEELPEFLPHTVETTPAWRKQ
                     QLPTATVLQNAGISLDAQKFIKNTGYIPPHFAKPILENMEAASLALHCSKNGIAFSAL
                     LCVTNTIGKEGRVEWLQNYRKAGEILTRLLLS"
     gene            4606..5679
                     /locus_tag="PLY93_04580"
     CDS             4606..5679
                     /locus_tag="PLY93_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68785.1"
                     /translation="MDSLTNASDAKKERRKLLISKLIHLSIVMAVATVLGVFAIKIIL
                     SHYKVEHKPMELGGDPKDFATHSRKGTFMKLSNLHFRLTDDISMNVKELIGEAIPKGG
                     VPFVNFDDVNSFRIDILSGEAFVKTEIMEYIFNNMVFNYDGSPLKNMKMQFFDDDEDG
                     KKVKKLRLTGDMKLVMWISFEMTGKMYLDREKTLMVIEAERIKSLGNPYTKGILDAVG
                     LNMEKLLPVPTGRGVTMIGNKIIVEPFKIFPPPQIGGYISDMRLHNDGLQLFFSSKTK
                     VNFPPLPVRNVKNYLYLYRGDVKFGKLTMVDARLQMVDMSQQNDFDFYLKKYQLPLAV
                     GYSKIMSDGSVVAHIPDYRDVAK"
     gene            5680..6132
                     /locus_tag="PLY93_04585"
     CDS             5680..6132
                     /locus_tag="PLY93_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68786.1"
                     /translation="MLRRYLKSTFLLLCAVAVLGCKQKFEIVPDGAQSVQRKDFVESL
                     KNSNAKVIRRDYQFIQNGSLFVCESHEFTRPEDAQKAAKEKIAERPTDEPKNSSGRFA
                     QSGRSLWLYTKNKLLWCMLASGKDENIAEAIKPVRASFIAELKKEGSP"
     gene            6129..>6430
                     /locus_tag="PLY93_04590"
     CDS             6129..>6430
                     /locus_tag="PLY93_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRP68787.1"
                     /translation="MKVLIVGAAAVSGRAAMKAARSLGATIVATTSRESNVDGADETI
                     YGVDLEKENAVEKILSDTRVKNIDYIVYIPARGAVGMDITESTAEMIPPSLAYSVEP"
BASE COUNT         1763 a         1471 c         1497 g         1665 t
ORIGIN      
        1 attgacccca aaagtgtcag ttatctctgt gggttgcata tctttaagac tgattatgct
       61 tgacccaaag ggcattttat cggctatgta cttcattaaa attatctgga gaatacaacc
      121 atgaatgtcg cccaattaaa agtaaatact tatgtagaag ctatagaaca atctttcaaa
      181 aataacggtc agcgtcttgc gctgcggtat cgagacggca gcgaggattt taaaacaatg
      241 acttattccg aactcgaagc tgaggtgcgc gtgattgccg caggtcttgc cgccatcggc
      301 gttaagccca aaaaccacgt tggtcttatc gccgacgtcg gccattattg gttgatgtca
      361 aatttagcga ttcaacttaa cggcgcagcg gacgtaccgc gtggcaccga ttccaccggt
      421 ccagagttgg cgtatatcgt cgaccactcg ggtgcagact tcgtgttggt ctacaacggt
      481 gagcaagtaa aaaaaattga agcgggtctt aaaaagcata aacatcgtgt aaaaaagtat
      541 attgtgctca acagcgatca caccgctaaa ggtgcaaacg tcatgacgct taacgatctc
      601 aaaaaagaag gtgaagctct catcaaaaaa ggtggtaaga cattggcgga atttgaatcg
      661 cgcgccggta aagtcaccgc atccgatttg tcgtgcttag tttatacctc gggcacgacg
      721 ggcgaaccca aaggcgttat gttgacgcat ggaaactttg cgtcgcaaat gcgcatcatg
      781 cctggcgttt taggcgcggg gaatgaagac agcggtctta ccttacttcc cccatggcac
      841 gtctttgggc gcattaccga aatgatgttt gtggctattg gcgcctcggt gacatacacc
      901 gacatcaaac acatcggtga agatttacgc gctgtgaaac ctacttatgt gcctgccgtg
      961 ccgcgtattt gggaaggtgt ctataataaa attattggca acgtcaagaa gcaaggcaaa
     1021 gagcccattt tcaataagtt caaaacagca gctcttaaat ttaatcatta ctcggcgatg
     1081 ctgaccggta aagaaaaacg tcccattaaa cgttcgttct tttctgccat tgtagcgcgt
     1141 ttggttggcc ttttgggtgt gttgctttgg ttgttaccta aaaaattggg ggatattctc
     1201 gttttcaaaa aagtcgttgc ggcgaccggg ggcaatctca aggcgtcgat ttccggcggt
     1261 ggagcgctcc cttcgtacat cgacgatttt ttccgtgcga ttggcgtgcg cattcttgaa
     1321 ggttatggtc taacggaaac ttcacccgtg ctttcggtgc gtcaaccttc gcgcattatc
     1381 cccgggacgg tgggcccgct catcgctgaa accgaataca aactcatcga tttagaaggc
     1441 aacgacgtga ctaaaattcc cggcgccaaa ggaactctcc acgtccgtgg accgcaggtc
     1501 atgcaaggct actataaaaa cccgaagaaa acggcagaag tcctcactcc cgatggttgg
     1561 tttaacaccg gtgatttagt cattataaac cattccggtg aaatttcgat tgtgggtcgt
     1621 agcaaggaca cactcgttct tgtcggcggt gaaaacgtcg aaccgacacc gattgaagaa
     1681 aaaatgaaag agagtccgtt tatcgatcac gtgatgctgg tttcgcgcgc attcggcgaa
     1741 gaccaaaaag tcttgggtgc gctcattgcg cccaacttgg ataatttggg tgagtggtgc
     1801 aaagccaaca acgttgcggg taacgcgact tcatggctta aagacgccaa agtgaacgct
     1861 ctttaccgcg ctgaaatcaa caagtacatc aatgctgaaa acggtttcaa aaattttgaa
     1921 aaagtgggag ttttcgctct tctgcctaaa ccgtttgagc aaggcgatga gttgaacaat
     1981 acactcaagg tgaaacggca tgtggtgaac gataaatacc acaagctcat cgacgagatg
     2041 tacaaggcgg ctaagtaaaa atataaaata cgcacaggct gcaacgcagt ctgtgcgttt
     2101 aacccatgtt actctctatt attatcgata gagttggcaa caccaacatc ttcaacatta
     2161 ttcaagaagg cgaggtgagt aacccccgtc ttaaagtgca tgaacgtttg cagtcgatta
     2221 tcgacgatga cctcatcgcc gagtatctcg aagagttggg acgcatcgcc aacatctcgc
     2281 gctcgctttc gtcgttaccc aaaggtagcg aagagaacca agcgcttatt tttcaacact
     2341 taaatttagt cgataaatta cgcgagattg gcagcgcgct ctttaaccag ttttttccaa
     2401 cgccattgca ggagtatatc cgcaatacag accctaattt tttatatttt cacgtcgacg
     2461 ctgcgctggc ttcgctgccg ctagagattt tccacgacgg cactgctttt ctttgggaga
     2521 aattttattt aggtaaagcg gtcaaaggcc aagacacaac gttcagcgaa ttgaatccgc
     2581 gcgaaaccat caatatgctc atcatcgccg accccagcga gaatttagag tgggcgcggc
     2641 gcgaaggaga acttcttttt gaacatttag gcgcgaacgt gaatccaaaa aaaattcatc
     2701 tcacgcttat cggcgggaaa agcgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 cttgaatgca attcttgata aagacattat tcattacgcc ggtcatttgc attaccatga
     2821 aagcgccggt gagaatgggt gggtccttgc tgacggtaaa attttttatg cacgcgaatt
     2881 taaaaagagc ggcgcgcaac caaaacttat tttttgtaat tcgtgcttga gcgcgcgttc
     2941 cgatcaacac gccaccgatt cgtcgtggta tgctcatttt gcctcggcgt ttatccgcgc
     3001 gggacgcacg agttacatcg gcaccaactg ggagctcccc gatcgaaagc cgacattaga
     3061 gttcaccacg catttttacg ataatgtttt caaaggaaag ccgctcggcg aggcgttgca
     3121 acacagccgc gtgtacgcca gagaacattt ttcgctcaac gatttgacgt gggcgtctta
     3181 tattctcatg ggtaacccgc aacaaacggt gtttcgtagc gagtcgctgt tgcccgatgt
     3241 cacgcacaac atccttgatc ccgacgtcgt cagtacgcaa tacccatacc ccattgccga
     3301 tgcgtttaag aaatttcatt ctattacaac gcagacggaa gggagtgcag aacaagccac
     3361 tgcgacactc cctgcgcttt ttacactctt tgagcgctcg gttttttttc tctcggcgtt
     3421 aattttagcc aattatcgta tttttaattt cccaaaaggc attgcgtttc cataccccga
     3481 cgttcaaaaa tgccttactg gactttttag cgcgttggga acgatacacg ctatcaaagc
     3541 gttgccgtta gcgacgaatt tattagagac gctatacgtg cataaagaaa atttattcaa
     3601 actggctaac ctagaaacaa gattccgtcg cgaaaatttg aaaaagagcg actatgaaac
     3661 atactccatt actgtgcagt atcatataga agcgctcttg acggatttag attttttaag
     3721 acattatggt ttttatttta tcgttgagcc cggccaccgt caactgagct ttcacggcac
     3781 cgaagaaaat cacacccatc gtgacatcct tttaccgaca caaacgaatt cggtagtata
     3841 cacagaaatt ttagagaaaa cttcactcct tgtcgggcgg tgcgtttttt attcgccggt
     3901 aaaaaaaatg tttttagatc tctcgccctt tatgcaaatc aacgccgacg ccgaaggtac
     3961 ctacactttt aactttacga aagcaaaagg cgagtgagta tttcgccagc cttgcggtaa
     4021 ttttgcagcc actcaacgcg cccttctttt ccaatcgtgt ttgtgacgca cagaagcgcg
     4081 ctaaacgcga taccgttttt tgaacaatga agcgcgaggc ttgctgcttc catattctcc
     4141 agaataggtt tagcaaaatg cggtgggata taccccgtat tttttataaa tttctgtgcg
     4201 tcaagactga tgccggcgtt ttgcaatacc gttgcggtgg gtaactgttg ttttctccat
     4261 gcgggggttg tttcaacggt gtgcggcaaa aattcgggca actcttcgcc ctctatcgat
     4321 ggaaatgtga actgggttgc gtggtatagt tgaaatatat cttctttata aagagagccg
     4381 caggtgccta cgagcacaac ttgttttggc ttttcaagcg taacgaggcg tgaaaattca
     4441 gccaaggcaa aaaattgccc cacgccgagt gcatacgggg tgaatcccac tcgagtaaat
     4501 ttttttaaat tatggatctc tttttcatgc gcaacgcaca accatgtctg catcaaacct
     4561 atattatgaa attgacacac cggtcaaaga ataaaaataa gtgtaatgga ctctctcact
     4621 aacgcctccg acgcaaaaaa ggaacggcgc aaacttctta tcagtaaatt gattcatttg
     4681 agtatcgtca tggcggtcgc caccgtcttg ggcgtctttg cgattaaaat tattttatcg
     4741 cattacaaag ttgagcataa gccgatggaa ttgggcggtg atccgaaaga ttttgcaacg
     4801 cactcgcgca aaggcacgtt catgaaactc tccaacctgc actttcgtct caccgacgac
     4861 atcagcatga atgtaaaaga acttatcggc gaagcgattc ccaaaggcgg cgttcccttt
     4921 gtgaactttg acgacgtgaa ttcattccgt atcgatattc tatcgggcga agcgtttgtc
     4981 aaaactgaaa tcatggaata catttttaac aacatggttt ttaattacga cggctcgcct
     5041 cttaaaaata tgaaaatgca attttttgac gacgacgaag acggtaaaaa agtaaaaaaa
     5101 ttacgtctca ccggcgacat gaaactcgtc atgtggattt ctttcgagat gacgggcaaa
     5161 atgtatttag acagagaaaa gactctcatg gtcattgagg ccgagcgcat caagtcgttg
     5221 ggaaatccct acaccaaagg catcctcgat gcggtggggc tcaatatgga aaaattgttg
     5281 ccggtgccaa cggggcgggg cgtgacgatg atcggtaata aaattatcgt cgagcctttc
     5341 aaaatttttc ctccaccgca aatcggcggt tatattagcg atatgcgcct ccataacgat
     5401 ggattacaat tgttcttttc aagtaaaacg aaagtcaact ttcccccttt acccgtgcgc
     5461 aatgtgaaga attacctata tctttatcgt ggcgatgtaa aattcggcaa actcacgatg
     5521 gttgatgcgc gcttgcagat ggtcgacatg tcgcagcaaa acgactttga tttctatctc
     5581 aagaaatacc aactaccgct tgccgttggc tattcgaaaa ttatgagcga cggttcggtg
     5641 gttgcgcaca tccccgacta ccgcgacgtt gcaaagtaag tgctccgcag atacttaaaa
     5701 agtacattct tgttgttgtg cgcggtggct gtgctcggtt gcaaacaaaa gtttgaaata
     5761 gtgcccgacg gcgcacaaag cgttcagcgg aaagattttg tagaatcact gaaaaattcc
     5821 aatgcgaaag taatccgtcg cgattaccaa tttatacaaa acggatcgct ttttgtttgt
     5881 gaatcgcacg agtttacccg tcccgaggac gcgcaaaaag ctgcgaaaga aaaaattgcc
     5941 gagcgtccca ccgacgagcc aaaaaattcc agtgggcgct ttgctcaaag tgggcgttcg
     6001 ttatggctgt acacaaaaaa taaattgctg tggtgtatgc tggcttcggg caaagatgaa
     6061 aacatcgcag aagcaataaa acccgtgcgt gcatcgttta ttgcagaact taaaaaagaa
     6121 ggttccccat gaaagtattg attgtcggcg ctgccgctgt gtcggggcgc gctgcgatga
     6181 aagcggcacg ctcgttgggg gcgaccattg ttgcgacgac aagccgtgag agtaatgtcg
     6241 acggcgccga tgaaaccatt tatggcgtcg atctggaaaa agagaacgct gtcgagaaaa
     6301 ttttatccga cacgcgcgtc aaaaatatcg attacattgt ttacatcccc gcgcgtggcg
     6361 ctgtgggtat ggacatcacc gaatcgaccg ccgagatgat accgccgtcg ttggcatatt
     6421 cggtggagcc
//
LOCUS       DAOMPD010000095         6388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5189_length_6388_cov_2.769304, whole genome shotgun sequence.
ACCESSION   DAOMPD010000095 DAOMPD010000000
VERSION     DAOMPD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5189_length_6388_cov_2.769304"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLY93_04595"
     CDS             <1..273
                     /locus_tag="PLY93_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP68788.1"
                     /translation="FVSVDTKNDTPAKLKEYLAPHPYARGYIGSDKEIQAVEKLLGAY
                     SKVEKDGIAHSLFVYVLNPQGKVVYLLSRNDSLEKLRTVVSEATRL"
     gene            288..677
                     /locus_tag="PLY93_04600"
     CDS             288..677
                     /locus_tag="PLY93_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRP68789.1"
                     /translation="MPAYSSALLSLAETPQVLIRAGNETCTLRNPLCGDCITLALEEK
                     KGRVENIEWQAEGCAILKASAALLAQTLRQKTKDEAKDLLLQFEASFEHENSFHQSPL
                     AAVYALPARYKCALLPWYAVKKLLGES"
     gene            complement(633..2603)
                     /locus_tag="PLY93_04605"
     CDS             complement(633..2603)
                     /locus_tag="PLY93_04605"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRP68790.1"
                     /translation="MSEQLSLAEHIALAPTSCGVYQWFEHKDDKTPLYVGKAKNVRAR
                     LKQYLISDDLKTTFLMRRAKFISWIATENETEALLLENNLIKKHRPQYNVRLKDDKQY
                     PYICVSMSEPFPRIYLTRRRIAGNLYFGPFSKAQLAREKIQLTLDLFPLRRRPLKLPL
                     PKPAKPCLNYHLKKCLAPCAWKISQEDYRKIVDEAVAFISGKNADSRRMVEERMQRLA
                     QELKFEEAARLRDVLEYFNERNVQPQVEMREAHLDFDIVGIYAADKSALEKELNVRDE
                     DMVLSPQNFSKDDTRAVRFAQICLLKFRDAKLIAKENFSLTETDLTASEFTHQDFMES
                     FFRDYYLNMLDLPPYIVVPNGLDLAAWQKPLSTHSATPPRVVTLDDFIDRIKKNFSEI
                     NPTALLQMAENNARLTMRERLLAETLRNQRVGLRQIQKFLGLKEMAQTIECYDISNIQ
                     GSDPVASGVMLKDGMPYKAGYRKYQIKTIEGANDPAMMHEVITRRLTRIKTGEMKAPD
                     LIVIDGGTTQLNAAMRARSEVSPTSTPIPMMGLAKQLEEIYTEDGRILRFDKESPGMH
                     ILRRARDEAHRFAVAYHRNRRMKRNLKSLFDGVAGIGEKKRAQILLLLRKLDLNTTEK
                     TELAKKIAKTTGVDIKEASALTEKLFNSIPRE"
     gene            complement(2634..3527)
                     /locus_tag="PLY93_04610"
     CDS             complement(2634..3527)
                     /locus_tag="PLY93_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRP68791.1"
                     /translation="MSRIALNLGGGAARGFAHIGIIRALIENDIRISHIVGISMGAIV
                     GGVYATMPNVDFLEKRMRQMVDFEAFSDSIVGTWTNTQETEAKGIVRRAQKLVTSTNI
                     LRRMFTASGVLSFDDVHAVLDPFLANIAIESTEIPFATAAVNIRDGALRVFQGKDRLR
                     NAVIASASMPLIFPPEKIGKDYFVDGGVLDKLGIDAAASIGINNPIVVDVSDEKLPDT
                     LPHSAFDVILKTEEIASEHRRKLQLEKASLVLRPIRGNFHWADYSAVTNFIEMGYECA
                     HENIRAIQHIASGRRLFALFR"
     gene            3582..4124
                     /gene="lepB"
                     /locus_tag="PLY93_04615"
     CDS             3582..4124
                     /gene="lepB"
                     /locus_tag="PLY93_04615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRP68792.1"
                     /translation="MQYRADYHRTTRRAGQKRWQAYILSSMLVIAVAFVLQFVLRYLF
                     FFPQRVTVDSMTPEISTGDKRYFIYPKLTKVGLGDVVLVKAAHADVEYLCRVAALDGD
                     KVQITEGKFWVNGVVKKQLKARLAITEDRSFQMAVVEVRPNYFFCLNDNAQNTNDSRT
                     HGPFERSQIIGKVFKPTLFF"
     gene            4124..5041
                     /locus_tag="PLY93_04620"
     CDS             4124..5041
                     /locus_tag="PLY93_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRP68793.1"
                     /translation="MNDAEKHELAKRFRERVAPFVKRHTALLPQEQSDAVLRQFTPDA
                     EELCYAEGFSDDPLHEEDADIHPFADVVHKYPNKILYLTTDECPVYCRYCTRKRKTLL
                     SAGHDATPLEKIVEYLRARPKIHEVIFSGGDPLMLPVHDLLHRMGVFLSMDSIREVRI
                     HTRAVTTAPNLISVKFLNRLKTLREKFPSKGIAFVLHINTAAEISIKATQKITALRAL
                     KIPSYAQSVLLKGINDDAAILAALCRALIYADVQPYYLHQLDRVTGAQHFEVDDGRAK
                     KIYNELKNLVPPYMLPRLMRDSKQGKHNL"
     gene            5104..>6388
                     /locus_tag="PLY93_04625"
     CDS             5104..>6388
                     /locus_tag="PLY93_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRP68794.1"
                     /translation="MNFLSKFYNTLIRGKIRFYLLLFCCYIGVRVFLAYNRWNDEEAA
                     VMQKMTLYKQQLDRLRSGTLGRDTIASIEPGAVAIPSKVFDRHGKIVGEFYTERRSLL
                     NYQDFSPHLIDALVASEDRKFYEHNGIDYAGIFRAMLRNLVHLRFVQGGSTLTQQLAK
                     VLFTQQEKTIDRKLFEFFCARAIETKYDKKEILEMYLNLIYMGHGIYGMETAAQFYFN
                     KSAAKLTVAEAAALVGLIPNPKAFSPLDDLPRFLARQNVVLKNLIATKKITEARAKSE
                     REKFFRVWRIASVKSTKSAIGDFPDRSIGKNYAPFFLDYLRQKLMSAFSQETLTRSGL
                     RIYTTLDLGRQIAAQKSVKAAVELQKQHYEKLLTAARKKNKAVKIQEFEKALATTNAA
                     FISIEPKTGYILAMVGGAEFSSQNQFNRALKAERQI"
BASE COUNT         1658 a         1541 c         1494 g         1695 t
ORIGIN      
        1 tttgtgagcg tcgatactaa aaacgacact cccgctaaac tgaaagaata cctcgcgcct
       61 cacccttacg cacgaggcta tatcggtagc gacaaggaga tccaagcagt ggaaaaactt
      121 ttgggcgcgt attctaaagt agaaaaagac ggtatcgcgc attctctctt tgtgtatgta
      181 ttgaatccac aaggaaaagt ggtttatctt ttaagccgga acgactcctt ggaaaaatta
      241 cgcactgtgg taagcgaagc aacgcggcta taatttttcc accacgaatg cccgcgtatt
      301 cttccgcact gctttccctt gccgaaacgc cgcaggttct aatccgcgca ggtaacgaga
      361 cttgcacgct gcgcaaccct ctttgtggcg attgtatcac attggcgttg gaggaaaaaa
      421 aggggcgtgt cgaaaacatc gaatggcaag cggagggatg cgctatcttg aaagcaagtg
      481 cggcgctctt ggctcaaacg ctccgccaaa agaccaaaga cgaggctaaa gacttgctct
      541 tacaattcga ggcatcgttt gaacatgaaa attcttttca ccagagcccg cttgctgcgg
      601 tgtacgcact ccctgcgcgg tataaatgtg cgctactccc ttggtatgct gttaaaaagc
      661 ttctcggtga gagctgacgc ttctttgata tccacccccg ttgtttttgc aattttttta
      721 gccagctccg ttttttctgt tgtgttcaaa tcgagtttgc gcaacaacaa caaaatctgc
      781 gctcgtttct tttcgccgat acctgccacg ccgtcaaaga gcgatttcaa gttacgcttc
      841 atgcggcggt tgcggtggta cgccacggcg aagcggtgtg cctcgtcgcg cgctcgacgc
      901 aagatgtgca tgcctgggga ttctttatca aaccgtaaga tacgcccgtc ttcggtataa
      961 atttcttcaa gttgctttgc aagccccatc atcggtatcg gtgtggatgt gggagaaact
     1021 tcactccgcg cacgcattgc cgcgttgagt tgcgtcgtgc cgccgtcgat gacaattaaa
     1081 tcgggagctt tcatctcgcc cgtcttgatg cgcgtaagcc gtcgcgtgat cacctcgtgc
     1141 atcatcgccg gatcgtttgc gccttcgata gttttaattt ggtatttgcg gtagcctgct
     1201 ttgtacggca tgccatcctt gagcattaca ccgctcgcca cagggtcgga gccttggatg
     1261 ttggaaatat cgtaacactc aatcgtttgc gccatttctt taagccccag aaatttttgg
     1321 atttgtcgca acccaacgcg ttggttgcgc agcgtctcag caagtaagcg ttcccgcatt
     1381 gtgaggcgcg cattgttctc agccatttgt aaaagcgcag tgggatttat ttcagagaaa
     1441 ttctttttta ttctatctat aaaatcatcg agtgtcacaa cgcgcggcgg cgttgccgag
     1501 tgcgtagaca acggtttttg ccacgcggct aaatcgaggc cgttgggtac aacgatgtac
     1561 ggcggcaagt cgagcatgtt taggtaataa tccctaaaaa acgattccat aaaatcttgg
     1621 tgggtaaact cactcgccgt taaatcggtt tcggtgagag aaaaattttc tttcgcgatg
     1681 agtttggcgt ctcgaaattt caacaagcaa atttgtgcaa agcgcaccgc gcgtgtatcg
     1741 tccttactaa aattctgtgg cgacagcacc atatcttcgt cgcgcacgtt cagttctttt
     1801 tctaacgcgc ttttatccgc cgcatagata cccacgatgt caaagtcgag atgcgcctcg
     1861 cgcatctcga cttgcggctg tacgttgcgc tcgttgaaat attcgagcac gtcgcgcaag
     1921 cgtgccgcct cttcaaattt taactcttga gccaagcgtt gcatccgctc ttcgaccatg
     1981 cggcgcgagt cggcattttt tcccgaaata aatgccaccg cctcatccac aattttgcgg
     2041 tagtcttctt gcgatatttt ccacgcgcag ggggcgaggc attttttgag atgatagttg
     2101 aggcagggct tggcgggttt gggtaaaggc aatttcagcg gcctgcgccg taacggaaat
     2161 aaatccaaag tgagctgaat tttttcacgc gccagttgcg ctttagaaaa cggaccaaaa
     2221 taaagattgc ctgcgatgcg ccgccgcgtc aaatagatac gcgggaaagg ttcactcatc
     2281 gatacacaga tatacgggta ttgcttatcg tctttgagac gtacgttgta ttgtggccgg
     2341 tgctttttga tgaggttgtt ttcgagcaag agcgcttcgg tttcattttc agtggcaatc
     2401 caactaatga actttgctcg acgcataaga aacgtcgttt tgaggtcgtc ggaaattagg
     2461 tactgcttga ggcgcgcgcg tacatttttt gctttgccga cgtagagcgg cgttttgtcg
     2521 tctttgtgtt caaaccattg gtatacgccg caacttgttg gcgcaagggc aatgtgttca
     2581 gctaaagaaa gctgttcgct catagggata taatactcaa atttttaata aggctatcgg
     2641 aaaagtgcaa acaaccgccg accgctggcg atatgttgaa tcgcgcgtat gttttcgtgc
     2701 gcgcattcat atcccatttc aataaaattt gtaacggccg aataatccgc ccaatgaaaa
     2761 ttccctcgga tggggcgcaa caccaagctc gctttttcta gttgcagttt gcgacggtgt
     2821 tccgatgcaa tctcttctgt tttcaaaatg acatcgaacg cgctgtgcgg tagtgtatcg
     2881 ggtaattttt catcggagac atcgaccaca atgggattat taataccgat ggaagctgcg
     2941 gcatcgatac ccagtttgtc aagcacgcca ccgtcgacaa aatagtcttt accaattttc
     3001 tccggtggaa aaataagcgg cattgacgcc gatgcaataa ctgcgttacg taatcgatct
     3061 ttcccttgaa aaacacgcaa cgcaccgtct cgtatattta ccgctgctgt ggcgaaagga
     3121 atttccgtcg attcgatggc aatgttcgct aaaaagggat cgagtaccgc atgcacatcg
     3181 tcaaaactca aaacgccgga tgcagtgaac attcgcctta aaatatttgt gctcgttacc
     3241 aatttttgcg cacgacgcac tataccttta gcctccgttt cttgtgtgtt cgtccaagta
     3301 ccgacgattg agtcgctaaa cgcttcaaag tcgaccatct gccgcatgcg tttttctaaa
     3361 aaatcgacat tgggcattgt tgcataaaca ccgccgacga tagcccccat cgaaataccg
     3421 acaatatgcg aaatacggat gtcgttttct ataagcgcgc ggataatgcc gatatgcgcg
     3481 aaccctcgcg ccgcgccacc gccgagattg agagcaattc tactcatacg aaaagtggta
     3541 atctgtttga cgaggtgaat tttttattgc tatcccctta catgcagtac cgtgccgatt
     3601 atcaccgcac cacgcgtcgc gcagggcaaa aacgctggca agcgtatata ctttcgtcga
     3661 tgttagtgat tgccgttgca tttgtattac aatttgtttt gcgctattta ttttttttcc
     3721 cacagcgcgt caccgtggac agcatgacgc cagaaattag cacaggcgac aagcgttact
     3781 ttatttatcc caagctgaca aaagttgggt tgggtgatgt ggtgctcgtg aaagccgcac
     3841 acgctgacgt cgaatatctt tgccgtgtgg cagcactcga cggcgataaa gtgcaaatca
     3901 cggaaggtaa attctgggtg aacggcgtgg taaaaaaaca actcaaagcg cgcctcgcta
     3961 ttaccgaaga ccgcagcttt caaatggccg tcgtcgaagt gcgtcccaat tatttttttt
     4021 gtctaaacga caacgcacaa aacaccaacg actcgcgcac acacggccct tttgagcgtt
     4081 ctcaaattat cggcaaagtg tttaagccta cacttttttt ctaatgaacg acgctgaaaa
     4141 gcacgaacta gccaagcgct tccgcgagcg cgtagcgccg tttgtgaaaa gacatacagc
     4201 gctgttgccc caagaacaaa gcgacgccgt acttcggcaa ttcactcctg acgcagaaga
     4261 actttgttac gctgaaggtt ttagcgacga tcccttacat gaagaagacg cggacatcca
     4321 cccgttcgcc gacgtggtgc ataagtaccc caataaaatc ttatacctca ccaccgacga
     4381 gtgtcccgtg tattgccgct attgcacgcg caaacgcaaa actctccttt cggcaggcca
     4441 cgacgctaca ccgttggaaa aaattgtaga gtatcttcgg gcgcgtccga aaatccatga
     4501 agtaattttt tcgggaggcg atccgctcat gctccctgtg catgatttgc tccaccgtat
     4561 gggtgtattc ttgtcgatgg attccatccg cgaagtgcgt atccacacgc gtgcagtgac
     4621 gacagcgcct aatttgattt ctgtgaaatt tttgaatcga ttaaaaactt tacgggaaaa
     4681 atttccatcc aaggggattg cattcgtttt gcatatcaac accgccgctg aaataagcat
     4741 aaaggctact caaaaaatta cggcgctacg cgcgttaaaa atcccatcgt atgcacaatc
     4801 ggtgctgctt aaaggcatca acgacgatgc ggcaattttg gccgcacttt gccgcgcttt
     4861 aatttatgcg gacgtccaac cttactattt acaccaatta gaccgcgtca cgggggcgca
     4921 gcattttgaa gtcgacgatg gccgcgcaaa aaaaatttac aacgagttaa aaaatttagt
     4981 gccgccttat atgctgccgc gtttgatgcg tgactctaag caagggaagc acaatttata
     5041 aaaaaacgta aatttaattt ttcaaaggtt gacgcataga gagcactgac atttaagggt
     5101 aacgtgaatt ttctatccaa attttacaat acattgattc gtggtaaaat tcgtttctac
     5161 ttactccttt tttgttgcta cattggcgtg cgtgtttttt tggcgtataa ccggtggaac
     5221 gacgaagaag cggcagttat gcaaaaaatg acgctctaca aacaacagct cgatcgcttg
     5281 cgcagcggca cgttggggcg cgacaccatc gcgtcgattg agccgggcgc ggtggcgatt
     5341 ccgtctaagg tgtttgatcg tcacggtaaa attgtcggcg aattttatac cgaacgacgt
     5401 agtcttttga actatcaaga tttctcgccg cacctcatcg atgcgctagt cgccagcgaa
     5461 gaccgtaaat tttacgagca caatggtatc gattacgcgg gtatttttcg tgcgatgctg
     5521 cgcaatcttg ttcatctacg ctttgtgcaa ggcggctcga ctctcactca acagttggcc
     5581 aaagttcttt ttacccaaca agagaaaacc atcgatcgta agctctttga atttttttgc
     5641 gcgcgcgcga ttgaaacaaa atacgacaaa aaagaaattt tagaaatgta cttaaaccta
     5701 atttatatgg ggcacggtat ttatggtatg gaaacggcgg cgcaatttta tttcaataaa
     5761 tcggcagcga agttgacggt cgccgaagcc gccgctttag tgggattgat accgaatccg
     5821 aaagcatttt cgccgttaga cgatttacct cgttttttag cgcgccaaaa cgttgtattg
     5881 aaaaatttaa ttgccacaaa aaaaattacc gaagcgagag ctaaatccga acgcgaaaaa
     5941 ttttttcgtg tatggcgcat cgcctcagtc aagagtacaa agtcggcgat tggcgatttt
     6001 cccgaccgct ctatcggtaa aaattacgcg ccgttctttt tagattactt gcgccagaaa
     6061 ttgatgtcgg cgttttcaca agaaacattg acccgcagcg gattacgcat ttataccact
     6121 ctcgatttag gcagacaaat tgctgcacaa aaatcggtga aagcagctgt agagttacaa
     6181 aaacaacact acgaaaaact actcaccgca gcgcgtaaaa aaaataaagc ggttaaaatt
     6241 caagaatttg aaaaagcgct cgccacaaca aacgcagcgt tcatcagcat tgaacccaag
     6301 acagggtata ttctggcaat ggtgggcggt gcagaatttt catcgcaaaa tcaattcaac
     6361 cgcgcgctta aagcagagcg gcaaatcg
//
LOCUS       DAOMPD010000096         6340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5232_length_6340_cov_2.615911, whole genome shotgun sequence.
ACCESSION   DAOMPD010000096 DAOMPD010000000
VERSION     DAOMPD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_6340_cov_2.615911"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLY93_04630"
     CDS             <1..181
                     /locus_tag="PLY93_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68795.1"
                     /translation="KAEGFAAEASTYDNTLQAQAREYLARLKSLWDIYVVRPHVALEK
                     PTPITGDGRQTWRKE"
     gene            complement(188..541)
                     /locus_tag="PLY93_04635"
     CDS             complement(188..541)
                     /locus_tag="PLY93_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRP68796.1"
                     /translation="MPFTLVGRILFALPLLVFAFGHFANAKMLLGIVPAWLPGGIIWV
                     YLVGVALAAGAIALLINKLTFQAAMGLALLLLTFALTVHLPALMKGNHASMMGLLKDI
                     ALAGAALYIAGHSRK"
     gene            910..1161
                     /locus_tag="PLY93_04640"
     CDS             910..1161
                     /locus_tag="PLY93_04640"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRP68797.1"
                     /translation="MPTNILRTLELPPMPQVAARVMQIDESQVSVSAEQIKNIIQADM
                     ALSTKLLKLANSAFYARGHRIQNLAQAIALLGFKTVKGL"
     gene            1224..1787
                     /locus_tag="PLY93_04645"
     CDS             1224..1787
                     /locus_tag="PLY93_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRP68798.1"
                     /translation="MDACSPHCSCLEKVGKDKIKDEVFMVGLLRQIGKSMLANQYPEE
                     YVICYDMAEEGVDEEKLHQLEKMKWGITSADASHVAMQTWNFPESLLNSSQVLRYNIT
                     NLEQLEKEQPHLLPVVIAEYIVLLGNYTNTIKLSEKLKSKLMDQVGKLSIIYGIDEEK
                     QRFYLDELRREIREDSFYSFCGELFTN"
     gene            complement(1754..2332)
                     /locus_tag="PLY93_04650"
     CDS             complement(1754..2332)
                     /locus_tag="PLY93_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP68799.1"
                     /translation="MNRFTQLITRTLLCAPLLGINLSIHAQNATATPPVSMIDNLTPL
                     ELEQADLAIQLAHSLARGKRYNEALHAYQEFMQVFAGSPRSREAHESMARIYELKQRY
                     DLAIREYNTLYRNLGVTSIGLLYRLEAARLYEIEGNENAAVAIYKELNRLDPHSTAAQ
                     QARTRMESLNLMQKVGDTEKDNQLVKSSPQNE"
     gene            2498..3061
                     /locus_tag="PLY93_04655"
     CDS             2498..3061
                     /locus_tag="PLY93_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68800.1"
                     /translation="MQNQKGVFEPVKDSGIILRDAPQLLTLSFPDAAQMEALAEDLKM
                     GQASRMSRLPPVSFVRFDFENKTSTAWRLELTPVFFTDAQGRKYTAYTEEKYNAHFTS
                     VAYEHYKYAKLYAAQILRRGQATPKDTLALRTYLPGVAVDVETQEAGFQVLPFTFIPA
                     GVEELTLYYLNPKNEAKSVKIGLKDKR"
     gene            3065..3685
                     /locus_tag="PLY93_04660"
     CDS             3065..3685
                     /locus_tag="PLY93_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRP68801.1"
                     /translation="MEVRLLIFVSLVLTAVLAKFFWRQGYVFLKLLPLLFIITHAFLF
                     SPRVDILWFAAISLGLAGDVLLLKEKWFVPGLLSFLLGHIFYIFAFNHESQQGFQPTL
                     VLFWVALFIIAVCGYFTRHLIQSRQKKYILPVILYVLVSGAFFASTLHNPLRSTAVLG
                     AFLFCFSDFLLGFHKFVRPMWYVQALVSLTYFAGQWLLAHHFGYVR"
     gene            3710..4030
                     /locus_tag="PLY93_04665"
     CDS             3710..4030
                     /locus_tag="PLY93_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HRP68802.1"
                     /translation="MEHVKITRVQVFSQEDGRNTLGIAHVTLSDAFVVKNLRLVKGRK
                     GIFIGMPSYRLRNGEFADVFFPVSREARMLLTNAVVDAFRHEFPHLLEGLQVYEADAA
                     ELTT"
     gene            4118..4396
                     /locus_tag="PLY93_04670"
     CDS             4118..4396
                     /locus_tag="PLY93_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68803.1"
                     /translation="MKKMMMVLAVAVFAATSLSAKMSAAEKECRKGANDTLKAAQVVH
                     KSSLGECKAKKGKDKVVCRKEANDILKNAKKTRNETIKNCKNAPAPAA"
     gene            4511..>6340
                     /locus_tag="PLY93_04675"
     CDS             4511..>6340
                     /locus_tag="PLY93_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRP68804.1"
                     /translation="MAAESFIATTKTVNICAHCGASSAFLKHANSFFCCDGCRRVYTI
                     LKEQNLLGYYEMGENTPLSLKGEAVHDFSYADSVWFHKTFTTPLSHGRYQMRLKLPAI
                     HCAATTLSRPLPSRPKPASTTAMNRSRKELTVVAQDSLSVSKLVQFVTDLGYTPDFKP
                     ESSRSRSLTQYDKGLLKRMAVAAFGFGNAMFFSLPDYLTHNLETRFQTLFITLNLSLA
                     LLVLFYSAGEFFTNAWRALKKKKIILDLPIALGIVAMFTRSVLEISVGVSSGYFDTFA
                     GLIFFLLIGRYVQSRSYTYLAFERDNLLFLPLAVRVVHEGAGEKTTPVQDLRTQDTIR
                     LLNGEIAPTRCRVLSDSVAVDYSFTTGESQPVILPKGDILEIGGKVVGHSALFSVMEP
                     VDQARVNRVWESATSDTAEISFTEESSFADKVLPYFTLIVLGIATAGFLYWLPQDTTM
                     AWNVFTAVVVITCPCALAMAKPFSFYTTLSALGRSGLFLKSAAVVEKFFNIGTIILDK
                     TGTITSAHEYDVTFHGVDNQILSEEAAVKIYSLTRESTHPLSKAITAHLAQNKDFIFA
                     DALAFEEVLGEGLSARVDGTLCVLGSRRYLESKNSTVTKSDADT"
BASE COUNT         1649 a         1399 c         1552 g         1740 t
ORIGIN      
        1 caaagccgaa ggttttgccg cagaagcgtc gacgtacgac aacacgttgc aggcgcaggc
       61 acgcgaatat ttagcgcgcc ttaagtcgtt atgggatatt tatgtggtgc gcccacacgt
      121 tgctttggaa aagccgacgc cgatcaccgg cgacggccgt caaacttggc gtaaagagta
      181 gtggtagtta tttgcgagag tgaccggcta tatagagcgc ggcaccggcg agagcaatat
      241 ctttgagtaa gcccatcatc gacgcgtgat tacctttcat gagcgctggt aaatgtacgg
      301 tgagtgcgaa agtgagcaaa agaagagcaa gccccatcgc tgcttggaag gtgagcttgt
      361 tgatgagtag cgcaatcgcg ccagcagcaa gtgcgacacc gacgagatag acccaaataa
      421 ttccaccggg caaccaagcg ggtacaatgc ccaaaagcat ttttgcgttc gcaaaatgac
      481 cgaaagcaaa aacaagcaag ggaagtgcga agagaatgcg tccaacgagt gtaaatggca
      541 tagtgactcc ttaaatattt aacatttatt tagatcttaa aatatataat aaatatcacg
      601 caatagctat aaatttattt gtgggtagat ttgagttgag gtggaagagc aaatattcct
      661 catcgttttg tggagggagt aagttggata cgcggtaatt tgtattatgt cacataatgg
      721 ggtaactatc cctaattatg tcacatagtg gggtaactat cccaagctga ttatgtcact
      781 ttaagggata actatcccta tcagaactta cttttacaag agtaacggca tttaggcact
      841 ttcaaaaccg tgccaaaaaa aataatgaaa tatttgacag gagcagagga aatatactca
      901 cggaagtcaa tgccaacgaa tattcttcgt actttagagc tgccccccat gccacaagtg
      961 gccgcgcgtg taatgcaaat cgacgaaagt caggtgagcg tttcagcaga gcaaataaaa
     1021 aatatcatcc aagccgatat ggcgctttcc accaaactct tgaaactggc gaactcagca
     1081 ttttatgcgc gcgggcaccg tattcaaaat ttagcgcagg cgattgcgct tttgggattc
     1141 aaaaccgtga aaggcttgta gctcttggtg tcggcggcgt ccattatacc caagaaaggt
     1201 aattttaaaa ttatgaaaga gctgtggatg cgtgcagtcc tcactgctct tgtctcgaaa
     1261 aggtcggtaa agataaaatt aaagacgaag ttttcatggt tggcttactt aggcaaatcg
     1321 gtaagtcgat gcttgccaac caataccccg aggagtatgt catttgttat gatatggcag
     1381 aagaaggggt ggacgaagaa aaactccacc aacttgaaaa aatgaaatgg gggattacct
     1441 ctgccgacgc ttcgcatgtt gccatgcaaa catggaattt tccggagagt ttactaaact
     1501 ctagccaagt attgcgttat aacatcacga atctcgagca acttgaaaaa gagcagccgc
     1561 atttgctgcc tgttgttatc gccgagtata ttgtgctttt gggtaactat accaacacaa
     1621 taaaattgtc cgaaaaattg aagtccaaat tgatggacca agtgggtaaa ctttctatca
     1681 tttacggcat tgatgaagag aaacagcggt tctatctcga cgaattacgc cgtgaaattc
     1741 gcgaagattc tttctattcg ttttgcggcg aacttttcac taactgattg tctttttctg
     1801 tatcgccaac tttttgcatc aagtttaagc tttccatgcg cgtgcgtgct tgttgtgccg
     1861 ctgttgaatg gggatcgaga cggttcaatt ctttatagat agccacagcc gcattttcat
     1921 tgccttcgat ttcatagagg cgcgccgctt ccaagcggta gagcagtcct atggaagtca
     1981 ctcctaaatt acggtagagt gtgttgtatt cacggatggc taaatcataa cgctgtttga
     2041 gctcgtaaat acgcgccata ctttcatgcg cttcgcggct gcgggggctt ccagcaaaga
     2101 cttgcataaa ctcttggtat gcgtggagtg cctcgttgta cctttttccc cgcgccaacg
     2161 agtgcgcgag ctggatggcc aagtcggctt gttcgagttc cagcggtgtc aaattgtcga
     2221 tcatactgac gggcggcgta gcggtggcat tttgtgcgtg gatggatagg tttatcccta
     2281 aaagaggagc gcagaggagg gtacgggtta ttaactgtgt aaacctattc atattatgtc
     2341 acatcggcaa atgagaaaat ttcataaact aaaaacctgc ttgccgtgct ttgtatcgat
     2401 tggatgagta cgcgtgcgac gtacgagttg gctttatttc ttcgttatcc tggcgtgtac
     2461 ccattgcgct gaaaataagc tcaccagagt gtacttcgtg caaaaccaaa aaggagtttt
     2521 tgagccggta aaggatagcg ggattatttt aagggatgcg ccacagttat tgacgctctc
     2581 gttccctgac gctgctcaaa tggaagctct ggcagaagac ttgaagatgg gacaggcgtc
     2641 acggatgagt cgtttacctc ccgtatcgtt tgtgcgcttt gattttgaaa acaaaacctc
     2701 gacggcatgg cgtttggagt taacccctgt ttttttcacc gatgcacagg gacggaaata
     2761 caccgcttat acagaagaaa agtacaacgc ccattttact tccgtcgcct acgagcacta
     2821 taagtatgca aagctgtatg cagctcaaat cctgcgtcgc ggccaagcga cgcccaaaga
     2881 taccttggcg ttgcgtacgt atctcccagg tgtggcggtg gatgtagaga cccaagaggc
     2941 gggcttccaa gtgttaccgt ttacgtttat cccagccggt gtagaagagt tgacgctata
     3001 ctatctcaac cctaaaaatg aagcgaagtc tgtgaaaatc ggcctcaagg ataaaagata
     3061 atttatggaa gtacgcctct taatttttgt gagccttgtt cttactgccg tgctggcaaa
     3121 attcttttgg cgacaggggt atgtttttct aaagctattg ccgcttcttt tcattattac
     3181 gcatgcgttt cttttttcgc cgcgtgtgga tattctttgg tttgcggcca tttcgctggg
     3241 gcttgccggt gatgttttgc tgctcaaaga gaagtggttt gtaccagggc tattaagttt
     3301 tcttttgggg catatttttt atatttttgc ttttaatcac gaaagccaac aaggattcca
     3361 accgacgctt gttttatttt gggtcgctct ctttattata gcggtgtgcg gctacttcac
     3421 gcggcattta atacagtcgc gccaaaagaa atatattctc cccgttatcc tttatgttct
     3481 cgtctctgga gcgttctttg ccagtacatt acataacccc ctacgctcaa cggccgtttt
     3541 aggcgcattc ctgttctgtt tttctgattt tttgttgggt tttcataagt ttgtgcgtcc
     3601 aatgtggtat gttcaggcgt tggtatcgct tacatacttt gcagggcagt ggctgttagc
     3661 acaccacttc gggtatgtac gttagcggta cgcagccgtg agggggtaaa tggaacacgt
     3721 taaaataact cgagtccagg tgttttcaca agaggacggg cgtaacacgc tggggattgc
     3781 acacgtcaca ctttccgatg cgttcgtggt gaaaaatttg cgtctggtta aaggtcgcaa
     3841 agggattttc atcggtatgc cgtcgtaccg gttgcgcaac ggcgagttcg ccgatgtttt
     3901 ttttcccgtt tcgcgcgaag cgcgtatgct tttaactaac gcggttgtcg atgcattccg
     3961 gcatgagttc ccacatttat tagaaggttt acaagtctat gaagccgatg cagcagagtt
     4021 aaccacgtaa gaaaaatctg tccggccaaa tccagcccat tcgtcttata catgtggtgt
     4081 ttcaaagatt ttctaaaaac acccacagga gaaaataatg aaaaaaatga tgatggtatt
     4141 ggctgttgct gtgtttgctg caacaagtct ttcagcgaaa atgagtgcag cagaaaaaga
     4201 atgccgcaag ggtgcaaacg atactttgaa agcagcgcaa gtggtgcaca aatcttctct
     4261 cggcgagtgc aaagcaaaaa agggtaaaga taaagtggtt tgccgtaaag aagcgaacga
     4321 tatcttaaaa aatgctaaaa aaacaagaaa cgaaaccata aaaaattgta aaaacgcacc
     4381 agcgcctgct gcctaaattg agctgtgttt gtattttttt gggcttgccc caaaaaatac
     4441 aaacatttcc ttagaatttt agaccattta atcctttgcc gcttgtttca aaaaaaataa
     4501 ggggttctat atggctgcgg agagctttat cgccacaacc aaaactgtaa acatctgtgc
     4561 gcattgtggc gcatccagtg cctttctcaa acatgcaaat tccttttttt gctgcgacgg
     4621 ctgccgtcgt gtttatacta ttctcaaaga acaaaactta ctcggctatt acgaaatggg
     4681 tgaaaatact cccttgtcgc tcaaaggtga ggcagtacac gatttttcat acgccgacag
     4741 cgtctggttt cataaaacat tcaccacgcc gctttcgcat ggccgctacc aaatgcgtct
     4801 caaattgccg gcgatccact gcgcggcgac aaccctgtca cggccgctgc catcgcgtcc
     4861 gaagccggcg tcgacgacgg cgatgaaccg atcaaggaaa gaattgacgg ttgtcgctca
     4921 agacagcctt agcgtatcta agctcgtaca atttgttacc gacttggggt acacccccga
     4981 tttcaaacct gagtcaagcc gcagccgctc gctcacccaa tacgacaaag gacttttgaa
     5041 gcgcatggcg gtcgcggcgt ttggtttcgg caacgcgatg ttttttagcc tgcccgatta
     5101 tttaacacac aatctggaaa ctcgttttca aactctcttt attaccctca atctttctct
     5161 cgcactttta gtgctttttt attcggcggg ggaattcttt accaacgctt ggcgtgcgct
     5221 caagaaaaag aaaataattt tagatttacc gattgcctta ggaattgtcg cgatgttcac
     5281 ccgcagcgtg ctggaaatta gcgtaggggt aagttctggg tattttgata cttttgcggg
     5341 gctcatcttt tttctcctca tcggccgcta tgtgcagagt cgttcgtaca cctacttggc
     5401 gtttgagcgc gacaatttac tttttttacc tttggcggta cgcgttgtac acgagggcgc
     5461 gggtgaaaaa acaacgccgg tacaagattt gcgcacccaa gataccatcc gtttgttaaa
     5521 cggtgagatt gctcccacgc gttgccgtgt actttccgac agtgtggcgg tggactactc
     5581 attcaccacc ggcgaatccc agcctgtcat tcttcccaaa ggagacatcc tcgaaatcgg
     5641 tggtaaagta gtagggcact ctgcgctctt cagtgtgatg gaacctgtcg accaagcgcg
     5701 cgtcaaccga gtgtgggaaa gtgcgacgag cgataccgcc gagatctcgt ttaccgaaga
     5761 aagttcgttt gccgacaaag tgttgccgta tttcacgttg atcgttttag gtattgcgac
     5821 tgcgggtttt ctttattggc taccgcagga cacaacaatg gcgtggaatg ttttcactgc
     5881 ggtggtggtg atcacttgcc cgtgtgcgct cgcgatggca aaaccgtttt cgttttacac
     5941 gacgctgagc gctttgggac gctcggggct tttcttaaag agcgcggcgg tggttgagaa
     6001 gtttttcaac attggcacga ttattttaga taaaaccggc acgataacga gcgcgcacga
     6061 gtatgatgtc actttccatg gcgtcgacaa ccaaattctt tccgaagagg cggcagtaaa
     6121 gatttattcg ctgacacgcg aatcgacgca ccctttaagt aaagccatca cagcgcattt
     6181 ggcgcagaac aaagatttta tttttgcaga tgcgcttgct tttgaagagg tgctgggcga
     6241 aggactttcc gcgcgcgtgg acggcacgct gtgtgtattg ggctcgcggc gctatcttga
     6301 aagtaaaaat agtacagtca cgaaaagcga tgcggacact
//
LOCUS       DAOMPD010000097         6309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5269_length_6309_cov_2.610329, whole genome shotgun sequence.
ACCESSION   DAOMPD010000097 DAOMPD010000000
VERSION     DAOMPD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5269_length_6309_cov_2.610329"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLY93_04680"
     CDS             complement(<1..695)
                     /locus_tag="PLY93_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRP68805.1"
                     /translation="MTDTLKRSLGPLAVWGMGVGYVISGMYFGWNLGLPQSGPFGLLG
                     ATLIIALMYIAFTLSYAELAAAIPRAGGVFVYTDRALGHYSGFLAGLAQNIEFVFAPP
                     AIAAAIGAYLTLFFPTVPPIAFAILAYILFTALNIWGVKESAIFGVFITVLAVVELLL
                     FAGLTLPHFSWAKFSTDALPSGWWGILPAIPFAIWFFLAIEGVANMAEEVKNPRRDLS
                     LGFKLAMATLFLLA"
     gene            complement(688..1302)
                     /locus_tag="PLY93_04685"
     CDS             complement(688..1302)
                     /locus_tag="PLY93_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68806.1"
                     /translation="MIYTLRTLVCFVLVFGASSLYALGGESLYVKAFTGGAYSSVVEK
                     ADDIKISFSGFSGLSYLQLGGALTPSLKIFGFTGVAVAPSPKAKTENLKIDTVYNMQM
                     LLDFGMGFAYYTKGGLFFSFAGSLAQNYYKFSVYGSDVGLYTRHGWGSQVMVGYEIPI
                     SPRWSYGVSGVFYYGQVKDTGPVPYNEAIVWNMYFGLVVSITYD"
     gene            1440..1928
                     /locus_tag="PLY93_04690"
     CDS             1440..1928
                     /locus_tag="PLY93_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HRP68807.1"
                     /translation="MLKLEQQVFPRWKQLSTEFGIDGAFEHFGPYVGANVKVCSVDAY
                     TVESSMALVETNTNYVGTHFGGSLYSMCDPFFMFILMRNLGNNYIVWDKAASIEFIKP
                     GTGTVTARFHIPPEEIEALKTELIRVKKLNRIYKCAVTDETGGFVAQMTKTLYIRRLN
                     LI"
     gene            2025..3668
                     /locus_tag="PLY93_04695"
     CDS             2025..3668
                     /locus_tag="PLY93_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRP68808.1"
                     /translation="MTSIIIQFALDIFVSSLYRPRGLRKRSFSRRFFAVFFRLILRRP
                     AGMVRFPVRLRQTIEKLGPTYVKLGQILSLREDLLPRRITYELRNLQTKVQPITYEEA
                     KMVVEQEFNVPLRHIFKEFAPKPVGAASLAQAHIAYLRNGQKVVVKVQRPGIVPIMTN
                     DLRIMQRLAWVLQQIPYIRDFQPRKLIQEFSDYTMKELDFNQEGKHADIFRENFKDQD
                     DVILPKIYWEYTTKKVLTLEFIEGIKPDDSEKLKKLGIDGPHVAALGARVVIKQLFID
                     GFFHGDPHPGNLFIVGRDRFCMIDLGMIGQFTPQTMNAMFLFYYYLIIHDFETASKYM
                     VSLTETIPASDIPGFRLEVEEIGKRWIGAGFKNYSLGKLILNSMNMGAKYKLYFNKDI
                     MLAVKAIVTIEAVGYILDPNMNLAKVSLPMMTEIFLSRISPMRMAKPMLRALPDYLDF
                     LERAPDTLLRTMGMFSSGKFQVEMIEKKLKKKPQDPVWKLWIPFASLATALLLLSADN
                     PPGGGVYITQGVQMPLISLVGFLITAWYAVRHWRSSIKV"
     gene            3929..5887
                     /gene="rpoD"
                     /locus_tag="PLY93_04700"
     CDS             3929..5887
                     /gene="rpoD"
                     /locus_tag="PLY93_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRP68809.1"
                     /translation="MVDLEQLPEIQKIIHIGKANGEITYDEINDILPEKLTNSDKIDD
                     VFILLNQYGIEIVEEYEKKQVGRRGDTSSAVVPGNFGSLGEQKVPKALISELKSQGYE
                     IKLPEGKAPLTIQDLAMQMLNRGYDLDALQILLQKPEFRPAAAKRKKTTGAAGASAAD
                     DPIRLYLKEIGKISLISGDKEVELARRIEGRENIIEDAVLRSSLLRSAFIKSYNKIDK
                     GTLRITDIARASKTYYISTTEQKELREKFLREIKPVIDYDKQISSLKAKLKRYTHKSK
                     KHQEVWAQILKLEGLASEHVIRVGVSQKELLKHVNKIKSMVFRIKEIKRHFLKLKERY
                     GHDVKGIKAFNRYIERNEDLHIVERELGCSVEDVKNIIKDIRNNERKLRRMEQEAGSP
                     TLIILLWGEKIARGSREIDSAKKELINANLRLVVSIAKRFANRGMHFFDLIQEGNIGL
                     MRAVEKFEYRKGYKFSTYATWWIRQAITRALSEQARTIRIPSHMIEQINKVNREARIF
                     LQETGREPSDDEIGERLGWPVVKVKQVKSVAKDPVSLETPVGEDEDSELGDFVEDKKA
                     PSPLQAAAQSILAEQLKHVLATLPAREQKVIRMRFGLDDGYTHTLEEVGYVFKVTRER
                     ILQIEAKALRRLRAPTRMRRLRDFLDQH"
     gene            5892..>6309
                     /locus_tag="PLY93_04705"
     CDS             5892..>6309
                     /locus_tag="PLY93_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRP68810.1"
                     /translation="MSPGDRAEKVFSLLTEEFGTPACPLNFKTIEQLAVAVILSAQCT
                     DARVNLVTPALFAHFPDMRSLAQAPIEQVEKLIFSTGFYHNKAKNITALAQILVRNYG
                     GKIPKDFLLLTTLPGIGRKTANVIMAEGFGEAPGITV"
BASE COUNT         1840 a         1505 c         1406 g         1558 t
ORIGIN      
        1 gccaaaagaa aaagcgtcgc cattgcaagc ttaaagccga gcgataaatc ccgccgcgga
       61 ttcttcacct cttcggccat gttcgccacg ccttcaatcg ccagaaaaaa ccaaatcgca
      121 aaggggatag ctggaagaat cccccaccac cctgaaggga gcgcatcggt ggaaaattta
      181 gcccaactaa agtgcggcaa cgtgagccct gcaaaaagca acaactcgac caccgcgagc
      241 acggtgataa agacaccaaa aattgccgac tccttgacgc cccagatatt gagcgcggta
      301 aaaaggatgt acgctaaaat ggcgaatgca attggcggca cggtgggaaa aaataaagtc
      361 aagtacgcgc caatcgctgc ggcgattgcc ggtggtgcaa acacaaactc aatgttttgt
      421 gcaagccctg cgagaaatcc agagtaatgt cccagtgcac gatcggtata aacaaacaca
      481 ccccccgcac ggggtatagc ggctgcgagc tcagcatagc tcaacgtgaa tgcgatatac
      541 atgagcgcaa tgatgagcgt tgcccccaaa aggccaaaag ggccactttg gggaagtccc
      601 aaattccaac cgaaatacat ccccgaaatg acatagccca cccccatgcc ccaaacggca
      661 agaggtccca ggctacgctt cagcgtatca gtcatacgtg atgctgacca ctaaaccgaa
      721 atacatattc cagactatcg cctcgttgta gggaacgggg ccagtgtctt tcacttgacc
      781 atagtagaac acacccgata ccccataact ccagcggggt gaaatcggaa tttcataccc
      841 caccatcact tggctccccc aaccgtgtcg agtgtataac cccacgtcgc tgccatagac
      901 agaaaacttg taatagtttt gtgcaagcga accggcaaat gaaaagaaaa ggccgccctt
      961 cgtgtagtaa gcaaacccca taccaaaatc caacagcatt tgcatgttat agaccgtatc
     1021 aatttttaag ttttcagttt ttgccttggg gcttggagcg acagcaacac cggtgaaacc
     1081 aaaaattttg agcgaggggg tgagcgctcc accgagttgt aaataactta aaccgctaaa
     1141 gccggaaaaa gaaattttaa tgtcatcggc tttttctacc accgacgaat acgcaccacc
     1201 ggtaaacgct tttacataaa gactctcgcc gcctaacgca tagagcgacg atgcaccgaa
     1261 aacaagcacg aaacaaacaa gcgtgcgtaa tgtataaatc ataaccaact atttctctct
     1321 taatatatat ctatagacaa gtcgccaagg ccgttttttt acatttgttg aattagacct
     1381 cgaggctatc gtaagagtta agatatttgt cgctgtgtat agactttttc gataggtaga
     1441 tgctcaaact cgaacaacaa gtcttccccc gttggaaaca gttgtctacc gaatttggta
     1501 tcgacggtgc ttttgaacat ttcgggcctt acgtcggcgc caacgtgaaa gtgtgctcgg
     1561 ttgacgcata cactgtggag tcatcgatgg cgcttgttga aaccaacacc aactacgttg
     1621 gcacgcactt cggtggttcg ctatactcga tgtgcgatcc tttttttatg tttattctca
     1681 tgcgcaattt agggaataac tatatcgttt gggacaaggc ggcgagtatc gagttcataa
     1741 aacccggcac ggggactgtc actgcgcgct ttcatattcc tcccgaagaa attgaagcgc
     1801 tcaagacaga gctcatccga gtcaaaaagc tcaatcgaat ttacaaatgc gcagtgaccg
     1861 atgaaacggg tggctttgtc gcacagatga ctaaaacact ctatattcga cggttaaact
     1921 taatataatt atcgaaaatt tcttgtcttg cccactcttg tattgaacta tgctttaagc
     1981 gcatgaactt gaagacctat gccgaaatac ggcgactatt ttctgtgacc tcaatcatta
     2041 tccagtttgc tctggatatt tttgtgtcaa gtctttatcg gccaagaggt ttaagaaaac
     2101 gatcgtttag ccgtcgtttt tttgcggttt tttttcggct tattctccgc cgccccgcag
     2161 gcatggtgcg ctttcccgtc cgcttacgtc aaacgattga aaagttgggg ccaacatacg
     2221 tgaagttagg ccaaatttta tcgctacgcg aagatttatt accgcgccgc attacttacg
     2281 aactacgtaa tttacaaact aaagtacagc ccattactta tgaagaagcc aaaatggtcg
     2341 tcgagcaaga attcaacgta cctttgcggc atatttttaa agaatttgct cccaagcccg
     2401 taggggcggc ctcgttggca caagcgcaca tcgcgtattt gcgtaacggc caaaaagtcg
     2461 tcgtaaaagt acagcgcccg ggaattgtac caatcatgac gaacgacttg cgtatcatgc
     2521 agcgtttggc ttgggtgctt caacaaattc cctatattcg tgattttcaa ccgcgcaagt
     2581 tgattcagga attttctgat tataccatga aagaactcga cttcaatcaa gaaggcaaac
     2641 atgcggacat ctttcgtgaa aattttaaag atcaagacga tgtaatttta cctaaaattt
     2701 attgggaata caccactaag aaagtattaa cgcttgaatt tatagagggt attaaacccg
     2761 acgacagtga aaaactcaaa aaattaggga ttgatggtcc acatgtcgcc gccttgggcg
     2821 cacgggttgt cattaaacaa ctttttatcg atggtttttt tcacggcgac ccacaccccg
     2881 gtaatttatt catcgttggt agagaccgtt tttgtatgat tgacctgggc atgattggcc
     2941 aatttacccc acaaacaatg aatgcgatgt ttctttttta ttattattta attatccacg
     3001 actttgaaac tgcatccaaa tatatggttt cacttacaga gacgatacct gcttctgata
     3061 tacctggttt tcgtttagaa gttgaagaaa tcggtaagcg ctggattggc gctggtttca
     3121 aaaattattc tttaggaaag ctcatcctta actcgatgaa tatgggggca aagtataaac
     3181 tttatttcaa taaagacatt atgctcgcgg taaaagccat cgtcaccatt gaagcggtgg
     3241 ggtatattct cgaccccaat atgaatttgg ccaaagtatc gctccccatg atgacagaga
     3301 tttttttaag ccgtatttct cccatgcgca tggcaaaacc gatgttgcgc gctctgcccg
     3361 attatttaga tttcttggag cgcgcacccg atacactttt gagaacaatg ggcatgtttt
     3421 ccagcggtaa atttcaagtt gaaatgatcg aaaagaaact taagaaaaaa ccgcaagacc
     3481 cagtgtggaa gctctggatt ccttttgctt ctttagcgac tgcactcctt ttactttctg
     3541 ccgataaccc gcctggtgga ggagtttata ttacgcaagg ggtgcaaatg ccgctaattt
     3601 cactcgtcgg ttttctcatc actgcatggt acgcggttcg ccattggcgt tcgagtataa
     3661 aagtatagaa agttagtaac tttttggcac ttgagatccg ccaaagtgcg ttttagttag
     3721 gcgataacat aaatttatcg tatattccag actcagaata aatgcaaaaa aaaataaaat
     3781 tagtttcgtt tgcgttgcgc aaaaaaacaa actatgcttg ttgcagtgtc tcaaaaatga
     3841 atactggcag aggtaagctt tagtgcgttc tttaaccgat attaaagtac ttctccaaga
     3901 aagctcaatc tttatctaaa gagggaacat agtggatctc gaacaacttc cggaaattca
     3961 aaaaattatc catatcggta aagcgaacgg tgaaatcacc tacgatgaaa ttaatgatat
     4021 tttacccgaa aaactcacca actccgacaa aatcgacgac gtttttattt tgctcaacca
     4081 atacggtatt gaaattgtcg aagagtacga aaaaaagcag gttggccgcc gtggcgacac
     4141 ttcgtctgca gtggtgcctg ggaattttgg ttcgttaggc gaacaaaaag ttcccaaagc
     4201 cctcatctca gaattaaagt cgcaggggta tgaaatcaaa ctccccgaag ggaaagcccc
     4261 tctaacgatt caagacttgg cgatgcaaat gctcaatcgt ggttacgacc tcgacgcatt
     4321 gcaaattttg ctccaaaagc ctgagtttcg ccccgcagcg gcgaaaagaa aaaagacaac
     4381 tggtgcagcg ggcgccagcg ccgccgacga tcccatccgt ctttatctca aagagattgg
     4441 taaaatatcg ctcatctcgg gcgacaaaga ggtggagctt gcccgccgca tcgaaggccg
     4501 tgaaaacatc atcgaagacg ccgtcttgcg ctcctcgctt ttgcgtagtg cgtttatcaa
     4561 atcgtataat aaaatcgaca aaggtacgtt gcgcatcacc gacattgcac gcgcttccaa
     4621 gacgtattat atcagcacaa ccgagcaaaa agagttgcgt gaaaaattct tacgcgaaat
     4681 caaaccggtt atcgactacg ataaacaaat ttcatctctc aaagcaaagt taaaacgcta
     4741 tacacacaaa agcaaaaaac accaagaagt ctgggcgcaa atcttaaagc ttgaaggatt
     4801 ggcttcggag cacgtcattc gcgtgggggt ttcacaaaaa gagctcctca aacacgtcaa
     4861 taaaatcaag agcatggtat ttcgtatcaa agaaatcaaa cgccatttct tgaagctcaa
     4921 agaacgctac ggccacgacg tcaaagggat caaagcgttt aaccgctaca tcgagcgcaa
     4981 cgaagatttg cacatcgtcg agcgcgagtt aggctgttct gttgaggatg tcaaaaacat
     5041 catcaaagac atccgtaata acgagcgcaa gctacgccgc atggagcaag aggcgggctc
     5101 gcccacgctt atcattctcc tctggggtga gaagattgct cgtggtagtc gcgagatcga
     5161 ctcggccaaa aaagaactca tcaacgccaa tttgcgcctt gtggtctcta tcgccaagcg
     5221 cttcgccaac cgtggaatgc atttttttga tcttatccaa gaaggcaaca tcggcctcat
     5281 gcgtgcggtg gaaaaatttg aataccgtaa aggctataaa ttctccacct acgcaacatg
     5341 gtggatccgc caagccatca cccgcgcgct ttcagagcaa gcacgcacca tccgcattcc
     5401 ttcgcacatg attgaacaaa tcaacaaagt caaccgtgaa gcacgtatct ttttacaaga
     5461 aacggggcgc gaaccttcgg acgatgaaat cggcgaacgt ttaggttggc cggtggtgaa
     5521 agtcaaacaa gtaaaaagcg ttgctaaaga tcccgtatcg ctcgaaacac cagtgggcga
     5581 agacgaagac tcagagctgg gcgactttgt cgaagataaa aaggcgccaa gccctttgca
     5641 agccgcggcg caatcgattt tagcagagca gctgaagcac gttcttgcga cactcccggc
     5701 gcgcgagcag aaggttatcc gcatgcgctt tggcttggac gacggttata cgcacactct
     5761 tgaagaggtg ggctatgtat tcaaagtcac gcgcgaacgt atcctgcaaa tcgaggcaaa
     5821 agcgttacgc cgtttgcgtg cgcccacccg tatgcgccgc ctgcgtgatt ttttagacca
     5881 acactgaggt gatgagccct ggggaccgag cagaaaaggt tttctctttg ctcaccgaag
     5941 agtttggcac accggcgtgt cctctcaact tcaaaactat cgaacagctc gccgttgcgg
     6001 tcattttatc agcgcagtgc accgacgcac gcgtcaattt agtcacccca gcactctttg
     6061 cacatttccc cgatatgcgc tcgttggcac aggcgcctat tgagcaagtg gagaaattaa
     6121 tttttagtac gggtttttat cataataagg ctaaaaatat caccgcactt gcccaaattc
     6181 tagtgcgcaa ttatggcggt aagataccca aggatttttt gttactaact acgctaccgg
     6241 ggattggccg caagacggca aatgtgatta tggcagaggg gtttggcgaa gcgccgggga
     6301 tcaccgtcg
//
LOCUS       DAOMPD010000098         6271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5308_length_6271_cov_2.953346, whole genome shotgun sequence.
ACCESSION   DAOMPD010000098 DAOMPD010000000
VERSION     DAOMPD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5308_length_6271_cov_2.953346"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /gene="ccsA"
                     /locus_tag="PLY93_04710"
     CDS             <1..415
                     /gene="ccsA"
                     /locus_tag="PLY93_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRP68811.1"
                     /translation="TVLVTGPLWAKPVWGVFWNWGDERLISFFVMWMMYNGYILLRAT
                     MTDIEKAARIGAVLALLAVVNAVLVVAAIYIWKTASHPGPLLVQQGGSTGLVNPMMQK
                     AFLLNLIAFTLLFLGILIARLRTEQGETQSHGFEL"
     gene            405..803
                     /locus_tag="PLY93_04715"
     CDS             405..803
                     /locus_tag="PLY93_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68812.1"
                     /translation="MNSKPLIYFLVFVGTLFPIAADFNTETLPDVLAQKVERYLGADT
                     AKARAGMVDFTNEDIEKITSAFKKEHSRSDQRLFWLNEEGYRRHAELASAERIYYLYL
                     AVLAALAIIAGFMLLTYRRARALVVASKEK"
     gene            complement(814..2193)
                     /gene="murF"
                     /locus_tag="PLY93_04720"
     CDS             complement(814..2193)
                     /gene="murF"
                     /locus_tag="PLY93_04720"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRP68813.1"
                     /translation="MFAKLNYDFFTIAAVCGIKMPSLNIEPVRITSITTSSLEVKPGS
                     LFVPLADKRDGHYFINDAIDHGAAAFFVRKGHNILKHIRASHQKKIILVDDPLQALNR
                     LAQFHRSRFAPYVIAVTGSNGKTTTKEMLAQIFQTAYANACTATKKNYNNHIGVPFTL
                     FSINEKTRVAIVEMGMNHAGEIASLTKIVKPHAALISSVGHAHIEFFSSRRAIAAAKG
                     EVMTAMPHGGQVYVPQDVQEMPTLLQIAKKHHCRIRRIDKRKGFLKIEKISPQGFTLS
                     IENKKFDFPYPNKAWVSNLALAAICAHDAGVKTTDILSAVRRFKPLDGRMQIVKGHFT
                     LIDANPDSAIASIDAALQIADGAPVVCVFADFKELGRFSQKLHAWTGIEAAKKGVAAF
                     YAIGSEMKHAQLAFGKNAAKSARSYFFAREDTARIIEALRAEKAGSVVLVKGSRAMKM
                     EEIVAQLKK"
     gene            2293..3057
                     /locus_tag="PLY93_04725"
     CDS             2293..3057
                     /locus_tag="PLY93_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68814.1"
                     /translation="MRVLIAGYGYLGRKIADIFLYKGASVTTISRHVQESALEKFEHI
                     IVDLTLQAPVLSFKKVDVAVFCLAPQPATDDDYTTTFVEAQKNFLKNIDAKRYIYVSS
                     IGVYPLQAGVYAERDAKPHSTKACILLDAEDLALNLGGTVLRLAGLYGVGRYIYNSKK
                     NFTEDKTLHLIHRDDAAYAVFHAVANCTDGAYNVHDGNTYWRSDLLSALCIAYSFPKG
                     GAMRIISAQKFFDTGYRISTQKNFMANVRMDAKEEL"
     gene            3059..4357
                     /locus_tag="PLY93_04730"
     CDS             3059..4357
                     /locus_tag="PLY93_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP68815.1"
                     /translation="MQRFRLLVVNVIFISALGAQASQNGKSDTQKSTRAVMTLTEINE
                     RVLARSEYLKAQKLQVQSVEAQGRQATAWHNPIVSAEIGRIRDPLNNSMTYDLRLTQP
                     FYFPGKLSAYGAIFEREAAFQKIQGNELAESLSLEAVYFAYAFSIAEQRASHSQDRIR
                     RISLMRTYMRAQAYASPAKLIQRNIVSTQLIALQKTLAEESAAVESAWQKLNVYLDQK
                     EKISVVTPWLIDLKDLNLEKFLEQVEKGNRRLKAKQMQIARLEAEESYEKRVPLPDVA
                     LSAFYRRETLEHPGINEFFGGMFSLPIPILNANRSAVEAAQKKVAAAKEEEKYLTREV
                     LHTARSLHAEYMHKKVLFDNFKEEKIPALERQMRYADRELKLGRIDLLSYLELELKTH
                     EAYVAYYDAQLELVKVITQMILLIGESSVYKGDLYVFQDN"
     gene            4338..>6271
                     /locus_tag="PLY93_04735"
     CDS             4338..>6271
                     /locus_tag="PLY93_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP68816.1"
                     /translation="MFSKIINAALENRSIVIGVFVLASIAGLFSITKLPVDAVPDITN
                     VQSMVITRTGALDPEKVESTVTYVIETELQGIPKVEEIRSVSKYGLSLITIVFEEDAN
                     IYLARQQISERLNTVKDALPDGITPELAPLSTGLGEVFFYVVEAKPDTPLAKLPERER
                     LIYLRTVQDYIIKRQLKSLKGVADVDTFGGYPKEVHVNLNMNKLNENGLTIEQVVERL
                     NTIGENYGGGYIQRDGKQIVIRTLGQMTNLDKIRQIPIKRSFFGAPIKLGEICEVTID
                     SPQPLGGATYKGEEAVIGMPMMLAGANSRNVARSINKAFKKLDLPPDVQVRALYSRDY
                     LVNATVKTVLKNLGEGAAFVVIVLLMILGNMRAAVLVSLAIPVSMLIAVIGMNYFHVS
                     GNLMSLGAIDFGLLVDGSVVMFENMIRRMEAHPASIPLKGEVKRKLILESTLEVLKPV
                     ITALVIIMGVFIPLFALEGVEGKMFRPMAQTVLLAMAASLVLAIVLMPVLGYLFFKLK
                     KGTQGASVEHHTPFILRYILKGYEPLLKFALRKPWLVITPTIALTFLSIALFFRMGGD
                     FIPQLNEGDAVINFNRTVSMSFSKSLDIQKASEKIIMGFPEVLEVFSRFGTSDAATDP
                     QGVFFSDTFLILEKDTKKWR"
BASE COUNT         1676 a         1359 c         1514 g         1722 t
ORIGIN      
        1 gactgtactc gtgaccggcc cactgtgggc aaagccggtg tggggagttt tttggaattg
       61 gggagacgag cgccttattt ctttttttgt catgtggatg atgtacaacg gttacattct
      121 tctgcgcgcc acaatgaccg acatcgaaaa agcggcgcgc atcggcgcgg tgcttgcgct
      181 gcttgcggta gtgaacgcag tgcttgtggt ggcggcaatt tatatatgga aaaccgcatc
      241 gcaccctggt ccccttttgg tgcaacaagg cggctcaacg gggctcgtca accccatgat
      301 gcaaaaggca tttttgttaa acttgattgc atttacttta ttatttttgg gtattcttat
      361 cgcacgctta cgcacagaac aaggggagac acaatcgcat ggctttgaac tctaaaccac
      421 taatttattt tttagttttt gtaggaacct tatttcccat cgccgccgat tttaacaccg
      481 agacactccc cgatgttctg gcgcaaaaag tggaaagata tctcggcgcc gatacggcaa
      541 aagcgcgcgc ggggatggtt gactttacaa acgaagacat tgaaaaaata acgagtgcat
      601 tcaaaaaaga gcattcgcgt tcagaccagc ggctcttctg gctcaatgaa gagggctacc
      661 gccgccacgc cgagttagcg tcggcagagc gcatttatta tctttacttg gctgttttgg
      721 cggcgctggc gattattgca ggtttcatgc tgttgacgta tcgtcgcgcg cgtgctttag
      781 tggtcgcttc aaaagaaaaa taatttaaat agattatttt ttaagctgtg ctacaatttc
      841 ttccattttc atcgcgcgcg aacctttcac gagtaccacc gaacccgctt tttctgcgcg
      901 taacgcttcg atgatccgcg ctgtgtcttc gcgcgcaaaa aaatagctgc gtgcgctttt
      961 tgccgcattt ttcccaaatg caagttgtgc gtgtttcatc tcgctaccga tcgcgtaaaa
     1021 agcggcgact ccttttttcg ctgcctctat gcccgtccat gcgtgtagct tttggctgaa
     1081 tcttcccaat tctttgaagt cggcaaacac gcacacaacc ggcgcaccgt cggcaatttg
     1141 taacgctgcg tcgatggacg cgattgccga gtcgggattg gcgtcgatga gggtaaagtg
     1201 gccttttaca atctgcatac gcccgtccag cggtttgaaa cggcgcacag cgcttaaaat
     1261 atctgtcgtc tttactcccg catcgtgcgc acatatagcg gcgagcgcca aattagaaac
     1321 ccatgctttg ttagggtagg ggaaatcgaa ttttttattt tcgatagaca atgtgaagcc
     1381 ctgtggagat attttttcaa ttttcaaaaa acctttacgc ttgtcaatcc ggcggatgcg
     1441 acagtggtgt tttttagcga tttgtaaaag cgtcggcatt tcttgtacat cttgaggtac
     1501 gtacacctgt ccgccatgag gcatagcggt catcacttcc ccttttgcgg cggcgatggc
     1561 gcggcgcgat gaaaaaaatt caatatgcgc atggcccaca ctgctgatga gcgcggcgtg
     1621 tggctttaca atttttgtga gcgatgcaat ttcgccggcg tggttcattc ccatttcaac
     1681 aatggcgacg cgtgtttttt cgttgatgga aaataaagtg aaaggaacgc cgatgtggtt
     1741 gttgtaattt ttttttgtcg cagtgcatgc attggcgtac gcggtttgga aaatttgcgc
     1801 aagcatttct tttgtcgttg tcttaccatt tgagccggtg acggcaatca cataaggcgc
     1861 aaaacgtgaa cggtggaact gtgccaggcg attcaacgct tggagcggat cgtcgacaag
     1921 gataattttt ttttggtgcg aggcgcggat gtgttttaag atgttgtgac ctttgcgtac
     1981 aaaaaaagcg gcggcgccgt ggtcgatggc gtcattgatg aagtaatggc cgtcgcgttt
     2041 atcggcgaga ggcacaaaaa gcgaacccgg tttgacttca agcgacgacg ttgtgatgga
     2101 tgtaatgcga accggttcga tatttaagga cggcatttta ataccacaaa ccgcagcgat
     2161 ggtaaaaaaa tcataattca atttagcgaa cataataagt acccatttac agcaaagagc
     2221 gtgtgcgaca accttaaaga tgtttaggac aaattagact atatagcgtg tcagggttat
     2281 agattatgac aggtgcgcgt tctcatcgcg ggatatggct atttaggtag aaaaatagcc
     2341 gacatttttt tatataaggg agcgagcgtg acgactattt cacgccacgt acaagagagc
     2401 gcgttggaaa aatttgagca tatcattgtt gatttaactc ttcaagcgcc tgttctttct
     2461 ttcaaaaaag tcgatgttgc cgtgttttgt ttggctccac aaccggctac cgacgacgat
     2521 tatacaacaa cattcgtgga agctcaaaaa aactttttga aaaatataga cgcgaagcgg
     2581 tatatttatg tttcaagcat tggtgtatat cctcttcaag cgggtgttta tgcagaaaga
     2641 gatgcaaaac cccattcgac taaagcatgc atccttttag acgcagaaga tctggcgctc
     2701 aatttgggtg gaaccgtatt gcgtcttgcg ggtctttatg gtgtagggcg atatatttat
     2761 aattcaaaaa aaaattttac cgaagataaa actttgcatt taatccaccg cgacgatgcg
     2821 gcgtatgcgg tctttcatgc cgtggcaaac tgtacagacg gagcgtataa tgttcacgac
     2881 ggcaatacgt attggcgcag cgatttattg agtgcgttat gcatcgcata ttctttcccc
     2941 aaaggcggtg caatgcggat tatttcagcg cagaaatttt ttgacactgg atacagaata
     3001 tcaactcaga aaaattttat ggcaaacgta agaatggatg cgaaagagga gttgtagaat
     3061 gcagcgtttc agattactcg ttgttaatgt aatttttatt tcagcacttg gagcgcaagc
     3121 ttcgcagaat ggaaagtccg atacccagaa gagcacacgc gctgtcatga cgcttactga
     3181 aataaacgaa cgcgtgcttg ctcgaagcga gtatctcaag gctcaaaaat tgcaagtgca
     3241 aagcgtggaa gcgcaagggc gccaagcgac agcgtggcac aatccaatcg tctctgctga
     3301 aattggccgc attcgtgatc cgcttaacaa cagcatgaca tacgatttgc gcttaacgca
     3361 gcctttttat ttccccggga aattatccgc atacggcgcc atttttgaac gcgaagcagc
     3421 gtttcaaaaa attcaaggga atgagttagc agaaagtttg tcgcttgaag ccgtttactt
     3481 tgcgtatgcg ttttctatcg ccgaacaaag agcctcgcat agccaagatc gtatacggcg
     3541 tatatctctg atgcgtactt acatgcgtgc ccaagcgtac gcttcgccgg caaagttgat
     3601 acagcgtaac attgtttcta cgcaactcat tgcgctacag aaaacactcg ccgaagaaag
     3661 cgccgccgtc gaaagtgcgt ggcaaaaatt aaatgtctat ctcgatcaga aagaaaaaat
     3721 aagtgttgtg actccatggc tcatcgatct taaagactta aatcttgaaa aatttttaga
     3781 gcaagtggaa aagggcaatc gccgcttgaa agccaagcaa atgcaaatag cgcgccttga
     3841 agcggaagaa tcgtatgaga aaagagtccc gttacccgat gtcgcactct ccgcatttta
     3901 tcgtcgtgaa acattggagc atcctggcat aaatgaattc tttggcggta tgttttcttt
     3961 accgatacca attttaaatg caaatcgttc tgcagtagag gccgcacaga aaaaagtcgc
     4021 ggcagcaaag gaagaagaaa aatatctaac gcgtgaagta ttgcatactg cacgctcgct
     4081 tcatgctgaa tatatgcaca aaaaagttct ctttgataac tttaaagaag aaaaaattcc
     4141 agcattggaa cgacaaatgc gttatgctga ccgcgagtta aaattaggac gtatcgattt
     4201 attatcttat ttagagctgg agcttaaaac gcatgaagcg tatgtcgcgt attacgatgc
     4261 acagcttgag ctggtgaaag tgatcacgca aatgattcta ctcattggtg aaagcagcgt
     4321 ttacaaaggg gatctctatg ttttccaaga taattaacgc tgcactcgaa aaccgcagta
     4381 tcgttattgg tgtatttgtt cttgcatcga tagcaggttt gttttccatt acaaagctgc
     4441 ccgtcgatgc cgtacccgac atcacgaatg tgcagtcgat ggtgattacc cgcaccggcg
     4501 cacttgaccc tgaaaaagtc gagtcgacgg tgacgtatgt aattgaaacc gaactacaag
     4561 gcatacccaa agttgaagaa attcgttcgg tttcaaaata cggcttatcg ctcatcacca
     4621 tcgtctttga agaagatgca aatatttatt tagcgcgcca gcagataagc gagcgcttga
     4681 acaccgttaa agacgccttg ccagacggca taacccccga attagcgccg ctgtcgacag
     4741 gtttaggtga agtgttcttt tacgtggtcg aagcgaaacc cgatacgccg cttgcaaaac
     4801 tacctgagag agaaaggctt atttatttgc gcacggtgca agattatatt attaagcgcc
     4861 agctcaaaag cttaaaaggc gtcgccgatg tcgatacgtt cgggggttat cccaaggaag
     4921 tgcatgttaa tttaaacatg aacaaactga acgagaatgg tttgaccatt gaacaggtcg
     4981 tcgaacgtct caacaccatc ggtgagaatt atggcggcgg ctatattcaa cgcgatggta
     5041 aacaaattgt catccgcact ttgggtcaaa tgacaaattt ggataaaatt cgacaaatac
     5101 ccattaagcg ttctttcttt ggagcaccga taaagctggg tgagatttgt gaagtaacga
     5161 tcgacagtcc acagcctttg ggcggtgcga cgtataaagg cgaagaagct gtcatcggta
     5221 tgccgatgat gcttgcagga gccaatagtc gtaacgtggc acgctctatc aataaagcgt
     5281 ttaagaaact cgacttgcct ccggatgttc aagtgagagc actctattca cgcgactacc
     5341 ttgttaatgc gacagtgaaa actgtactta agaatttggg tgaaggcgcg gcatttgtcg
     5401 tcattgttct cttgatgatt cttggcaaca tgcgcgcagc ggttcttgta tcgttggcga
     5461 ttcccgtgtc gatgctcatt gccgtcatcg gtatgaacta ttttcatgtg tcgggaaatc
     5521 tcatgagttt gggagccatc gactttggct tactcgtcga cggttcagtc gtcatgttcg
     5581 agaacatgat tcgacgtatg gaagcgcacc ccgcctcgat accgcttaaa ggcgaagtga
     5641 aacgaaaact cattttagaa tcaacgctcg aggtattgaa gccagttatc actgcgctgg
     5701 tgatcatcat gggcgtattt attcctcttt ttgcgctcga aggcgtcgaa ggtaaaatgt
     5761 ttcgtcccat ggcgcaaacg gttcttttag cgatggcggc ttctcttgtg ttggcgattg
     5821 tgctcatgcc agttttaggt tatcttttct ttaaactaaa aaaaggaaca caaggtgcaa
     5881 gcgtagagca ccatacaccc tttatattgc gctatatttt gaaagggtac gagcctttac
     5941 tgaaatttgc tctcagaaaa ccttggcttg ttattacccc tacgatcgcg ttgacatttc
     6001 tgtctatcgc tctttttttt cgtatgggcg gtgattttat tccgcaactc aacgaaggcg
     6061 acgcggtcat caactttaac cgcaccgttt cgatgagctt tagcaagtcg ctcgacatac
     6121 aaaaagcatc tgaaaagatt atcatgggat ttcccgaggt gctggaagtt ttttcacgct
     6181 ttggtacatc cgacgcggcg actgacccgc aaggtgtttt cttttcagat acatttctca
     6241 ttttagaaaa agacacaaag aagtggcgta a
//
LOCUS       DAOMPD010000099         6257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5325_length_6257_cov_2.685585, whole genome shotgun sequence.
ACCESSION   DAOMPD010000099 DAOMPD010000000
VERSION     DAOMPD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5325_length_6257_cov_2.685585"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLY93_04740"
     CDS             complement(<1..780)
                     /locus_tag="PLY93_04740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRP68817.1"
                     /translation="MQRILIIAKQSLGDIILQLGVIQALKEKNPAVQIDVACYEKYRP
                     ALEKQSEIAEILTLDLASGKSRGFFTVLKQIKQKHYDIILSFGPSKQAVWWGFLARIK
                     IRIGTANQPFGFLLTRSLRERDTQLNSFEYYFKLAQLLAPGLSVAFPKIPIEKKETQH
                     LRASLYKQKVARKFIVWHIGSSLEEKRYPIFQIVETLRLFHKKRITLPVVFAGGRGDE
                     RFLEELEATVNAANIRTTKVFFADTLQFQATAALFSLAHLVI"
     gene            complement(811..1881)
                     /locus_tag="PLY93_04745"
     CDS             complement(811..1881)
                     /locus_tag="PLY93_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRP68818.1"
                     /translation="MFEAALGEYTGAIAFVFAFQVLPTIIFVASIFSVLYYLGVMQPI
                     IRHIAAFINRFMKASGAETLDVAANIFMGQTEAPLTIKPYLAHLTRSELFTVMVSGMA
                     HCSAAILIVYVSVAGVDAKHLLASVIMTAPGAIMLAKLVIPETGTPQTASSNSLNNIS
                     NKGETNQNTKDENAPVNAVDAAARGASEGMILAANVAAMLIAFIALIALFNGTWSSLR
                     TYGIAHTSGTLQSVIAAVPDSIQQILGYLFAPIAWVSGVPWNEAVQVGNLLGTRIVLN
                     EFVGYIELGKIKAVLSAKSFLISTYLLCGFGSLTSIAIQAGGIGALVPTRRAELAELG
                     LKAVLVGVLSNVLAASIAGILF"
     gene            complement(1874..2308)
                     /locus_tag="PLY93_04750"
     CDS             complement(1874..2308)
                     /locus_tag="PLY93_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HRP68819.1"
                     /translation="MPFSNYTGVIGILFVVGICYVFSTNRRAIDWRTIIWGLVLQIVF
                     ALLIIRGESIAHLFDFLPITHGTLLAIVLLQLLAIYTILKFRKIWTSKLSVVWIKRVV
                     LFELTIYVLKFNLVGVLFDALKTGATQLLNFSTVGATFVFGV"
     gene            complement(2310..2732)
                     /locus_tag="PLY93_04755"
     CDS             complement(2310..2732)
                     /locus_tag="PLY93_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68820.1"
                     /translation="MPFKIPVQLYIIHFALMLSPAFLAFALYFIVESQPTDIAGSTED
                     IPVYQFTAAILAIIAVAFAQLTPRFITREKSTLPMQRYVSMKIIQWALVEMAAIFIGI
                     TYFLTHQANMLVPMGILIAVIAFMRPTVDELMRYNVKG"
     gene            2869..3036
                     /locus_tag="PLY93_04760"
     CDS             2869..3036
                     /locus_tag="PLY93_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68821.1"
                     /translation="MKKLATILMFAAAMSFVACSSTEEKKAEAPTEPAKTEAPAKAPA
                     KAPAKAPAKKK"
     gene            3118..3978
                     /locus_tag="PLY93_04765"
     CDS             3118..3978
                     /locus_tag="PLY93_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP68822.1"
                     /translation="MERHFFKFQNLSLSYLEAGQTHSKKVLIAHANGYAAGMYAYLID
                     ALKTRYHVCALDFSGHGESQSTLEFNSWDFFRDQIFALMNHKEWHTCVGVGHSLGGGS
                     LIRAAIQDSSRFEKIIAFDPVVLSHLAIFYIYLFGNSMARAALARRAVFKSKEQAYKI
                     FDRHPANKSWQRESVRAYIDYCIRETANGAELNCSPAVESRIFSLKECAHLSKLKNIT
                     AEIHLILPPQSHVCPPRAGRHITSGNLYSTVERVANSGHLLPFENHAFVVSTVNSFLV
                     SKSTVTPSLF"
     gene            complement(4012..>6257)
                     /locus_tag="PLY93_04770"
     CDS             complement(4012..>6257)
                     /locus_tag="PLY93_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRP68823.1"
                     /translation="DVTRLYFPRVEKSDAVFVYTTGVSKRYLVMINNSAQEQRVHFSS
                     DVFLYFFDLNEKSDLLVYKNIIANQEEIRTKKECVEKGAVFFLQPYASCVLTHFTTRA
                     ETVRDNYPRIGDYLAGVAAPVCALRSQQSCAIGEFYDLKILIDWCVATGQRIINLLPV
                     NDTGYNSSPYSALSAMALHPVYLSLDNFPLCDDLRQRVEAEKKRTRAFSRVIFYDIVR
                     FKEDILQEHYNAAPDAAMEDVPHNFKNQEWVRDYAIFRILKKECNGNAWFNWEKHTAT
                     EEILRLIENPTATARQHFFEKNQREANFYIWVQYQLDLQLSQVRDYARERGVFLKGDI
                     PILLERDSVDVWLNPHLFNSQLRAGAPPDMFSPDGQNWGFPIYAWNEHAKENFAWWRA
                     RIRQMARYFAAYRIDHVLGFFRIWAIDENNTTGIDGFFVPSYALAREDFYALEVSDVS
                     LALLSRVRLYEHELELIRAAGNESVESIDGLHFLSHAYAHERGIHALRLEENFTHELL
                     RRLHTRAFLEYEKKFYPLWDFEKRLWHICDIDDSLRIRIQEKIRVLKTSEADEQRGHG
                     RKILSVLKEESGMLPCAEDLGVVPDYVRPTLEALQILCLKVYRWEAENNIPKDPKSYP
                     YLSLATSSVHDSSNLREWLMTEPSSLTGIEIHNGKVSAQNVYDFLARLYASPSMITMV
                     PLQDLLALDGSLHGDAIEERINIPGTVSDFNWTYRMPITLEELLQNTALCEKIKNLAL
                     LKNSHRE"
BASE COUNT         1696 a         1385 c         1500 g         1676 t
ORIGIN      
        1 gatgacaagg tgcgctaggg agaagagcgc agcggtcgct tggaattgta gtgtgtcggc
       61 aaaaaacact tttgtcgtgc ggatattggc ggcgtttact gttgcttcga gttcttctaa
      121 aaaacgctcg tcgccgcggc caccggcaaa aacgacgggg agcgtgatac gttttttgtg
      181 gaatagacgt agcgtttcca caatttgaaa aatggggtag cgtttttctt ccaaactgga
      241 gccgatatgc cacacaataa acttacgcgc gactttttgt ttatagaggc tcgcgcgtaa
      301 gtgttgtgtt tccttttttt cgatggggat tttggggaat gcgacggaca atccgggagc
      361 caagagttgt gcgagcttga aataatactc gaatgaattg agttgggtat ctctttctct
      421 caacgaacgg gtgagtaaaa agccaaacgg ttggtttgcc gtaccgatgc ggattttaat
      481 gcgcgctaaa aatccccacc aaactgcttg tttacttggg ccgaaactga gtatgatgtc
      541 gtaatgcttt tgtttaattt gcttgaggac ggtaaaaaat cctcgacttt tacccgacgc
      601 taaatcgaga gttaaaatct ctgcaatttc ggattgcttt tctaaggccg gtcgatattt
      661 ttcgtaacac gcgacatcga tttgtactgc cggatttttt tctttgagtg cctgaatcac
      721 accgagttga agaataatat caccgagtga ctgtttcgca ataattaaga ttcgttgcat
      781 gcacgcctcc ttggcggttt tcgcaccgca ctaaaaaaga atcccagcga tggacgccgc
      841 caaaacatta gacaagacgc ctaccaaaac agcttttaat cccagttcgg caagttcagc
      901 gcggcgggtg ggtacgagcg cgccaatgcc accggcttgg atggcaatcg aggtgagcga
      961 accgaagccg caaagtaggt acgttgaaat gagaaatgat tttgccgaaa ggacggcttt
     1021 gattttcccc aattcgatat accccacaaa ctcattgagg acaatgcgtg tacccaaaag
     1081 attgcctacc tgcactgctt cgttccacgg tacgccactc acccatgcta tcggcgcaaa
     1141 gagatatccc aaaatttgtt ggattgaatc cggtacagcg gcaatcacgc tttgtaacgt
     1201 accactggtg tgcgcgatgc catatgtacg cagcgaagac catgtcccgt taaataatgc
     1261 aatgagcgca atgaatgcga tgagcattgc tgcgacgttg gcggctaaaa tcattccttc
     1321 cgaggctcca cgtgcggcgg cgtccaccgc attcacgggg gcgttctcgt cttttgtatt
     1381 ttgattggtt tctcctttat tagagatatt gttgagcgaa ttactactgg cggtttgggg
     1441 ggtgcccgtt tcggggataa ctaactttgc gagcatgata gcccctggtg cggtcataat
     1501 caccgacgct aaaagatgtt ttgcatcgac acccgctacc gacacgtaca caatgaggat
     1561 tgccgcgcta cagtgcgcca tccccgacac catcacagtg aaaagttcag aacgagtgag
     1621 atgcgcgagg tatggtttga tggtgagtgg cgcctcggtt tgccccataa agatattagc
     1681 cgccacgtcc aaggtttctg caccggaggc tttcataaaa cggttgatga aggcagcaat
     1741 gtggcggatg atgggctgca tcacacccag ataatagagt acagagaaaa tcgacgccac
     1801 aaaaataatg gtgggtaaga cttgaaaggc gaatacaaac gcgatagcgc ccgtatactc
     1861 tccgagcgct gcttcaaaca ccaaagacaa acgttgcgcc gacggtggaa aaattgagga
     1921 gctgcgttgc ccctgttttg agcgcatcaa agagcacccc cacaagattg aacttgagta
     1981 cataaatggt taattcaaaa agaacaacgc gcttaatcca aacgacagaa agtttactcg
     2041 tccatatttt tctaaatttg agtatagtat aaatcgccaa tagttgcaac agtacgattg
     2101 ctaaaagtgt accgtgtgta attggcaaaa agtcgaacag gtgtgcgata ctttctccac
     2161 ggatgataag gagcgcaaaa acgatttgca aaacaagacc ccaaattatg gtgcgccaat
     2221 cgatggcgcg acggtttgtc gaaaagacgt agcaaatccc aacgacgaag agaataccga
     2281 tgacgcctgt atagtttgaa aatggcatat taacctttaa cgttatagcg cataagttca
     2341 tccaccgttg gacgcataaa ggcaatcact gcaatgagta tacccatggg cacaagcata
     2401 ttcgcttgat gcgtcaagaa ataggtaatc cctataaaaa ttgccgccat ttcaacgaga
     2461 gcccactgaa taattttcat cgaaacataa cgttgcatcg ggagcgttga tttttcacgt
     2521 gtgataaagc gcggcgtgag ttgcgcaaac gccacagcga taatcgccaa aatagctgcc
     2581 gtgaattggt acactggaat gtcttcggta ctgccggcaa tgtcagtggg ctgcgattcg
     2641 acaataaaat aaagtgcaaa agcaagaaag gcgggcgata gcattagagc aaaatggatt
     2701 atataaagtt gcacgggtat tttaaacggc ataaagaccg tatgtatcat aagagtctat
     2761 acgccaaccg taaatatttt ttgtgtatga actcctttac actatatcaa aattatgaat
     2821 ctcgactatg ttcttgaaaa agaataattt ttcgtacgag gaaattacat gaaaaaattg
     2881 gcaacaattc ttatgtttgc tgctgcaatg tcattcgtgg cttgctcatc aactgaagaa
     2941 aaaaaagctg aagcgcctac agaaccagca aaaactgaag cgcctgcaaa agctccagca
     3001 aaagcgcctg ctaaagctcc tgcaaagaag aagtaagcaa gcttaaggtt cgtaaaacac
     3061 gacgactgta agtcgtcttt tttacgagcc tttttttatg ccctaaccgt agctatcatg
     3121 gaacgccatt tttttaaatt tcagaattta agtctttctt atcttgaagc aggacaaact
     3181 cactcaaaga aagtactcat tgcgcacgcc aatggttatg cggcgggtat gtatgcatat
     3241 ttaatcgacg ctcttaaaac gcgttatcat gtctgtgcgc ttgatttttc ggggcatggc
     3301 gagtcacaaa gcacgcttga gtttaattca tgggattttt ttagagacca gatttttgct
     3361 ctcatgaatc ataaagagtg gcacacttgt gtcggtgttg gtcattcgtt gggaggtggt
     3421 tcgttgattc gagctgcaat ccaagattca agccgttttg aaaagattat cgcatttgat
     3481 cctgttgttt tgagccatct tgcgattttt tatatttatc tttttggtaa ttcgatggct
     3541 agggcggcgt tagcgcgccg tgcggtattt aaaagtaaag aacaagcgta caagattttt
     3601 gatcggcatc cggcgaacaa atcatggcag cgagaaagcg tgcgtgcgta tattgattat
     3661 tgcatccgtg aaacggctaa cggcgctgaa ttaaactgtt cacccgccgt agagtcgcgc
     3721 attttttctt tgaaagaatg cgcgcattta tctaaactaa aaaatatcac cgcagagata
     3781 catttaattt tgccacccca atcgcatgtt tgtccaccac gcgctggacg gcacataaca
     3841 agcggaaatt tgtattctac tgtcgagcgt gttgcgaatt cagggcatct attgccattt
     3901 gaaaaccacg cttttgtggt gagtacagta aactcttttc ttgtttcaaa gtcgaccgtg
     3961 accccttctt tattctaact cagcgctatt aagaattaat tccttgttct attattctct
     4021 gtgcgagttt tttagcagcg ctaagttttt aattttttca cagagtgcgg tgttttggag
     4081 caattcttca agcgtaatcg gcatgcggta agtccaattg aaatcgctca cagtgcctgg
     4141 gatattgatg cgctcttcta tagcatcgcc atggagcgag ccatcgagcg cgagcaaatc
     4201 ctgtaacggc accatggtta tcatcgatgg cgatgcgtaa agacgcgcga ggaaatcgta
     4261 cacattttgc gcggatacct tgccgttgtg gatttctata cccgtgagag aactcggctc
     4321 ggtcatgagc cactcgcgta aattcgacga gtcgtgtact gagcttgttg ctaaggagag
     4381 atacggatag cttttcggat ccttggggat attattttct gcttcccacc gatatacctt
     4441 gaggcataaa atttggagtg cttcgagagt gggacgcacg taatcgggca cgacgcccaa
     4501 gtcttcggcg caaggcagca tgccgctttc ttctttgagt acagataaaa ttttgcgtcc
     4561 atgtccgcgc tgctcatcgg cttcgcttgt tttgagaacg cgtattttct cttggatgcg
     4621 tatacgcaaa ctatcatcga tgtcgcaaat atgccacagc cgcttctcaa aatcccataa
     4681 gggataaaat tttttttcgt attcaagaaa tgcgcgggtg tgcaaacgac gcaagagctc
     4741 gtgcgtaaaa ttttcttcga gacgtaatgc gtggatgccg cgttcgtgtg cgtatgcatg
     4801 ggaaagaaaa tgcaagccgt cgatgctctc cacgctttca ttgccggcgg cgcggattag
     4861 ttctaattca tgttcgtaaa gacgcacgcg gctgaggaga gcaagcgata catctgaaac
     4921 ttccaatgcg taaaaatctt cacgcgccaa agcgtaagag ggcacaaaaa atccatcgat
     4981 gccggttgta ttattttcgt cgatagccca aatacggaaa aagcccagca catgatcgat
     5041 acggtacgcg gcaaagtagc gcgccatttg gcggatacgt gcacgccacc atgcaaaatt
     5101 ttcttttgca tgttcgttcc acgcgtaaat ggggaatccc cagttttgtc catcgggcga
     5161 aaacatatcg ggcggtgcac cggcgcgcag ttggctattg aacaaatgtg gattgagcca
     5221 gacatcgacg ctatcacgct ccaagagaat gggaatgtct ccctttaaaa aaacaccccg
     5281 ctctcttgcg taatcgcgta cctgtgaaag ttgaaggtct aactggtact gcacccaaat
     5341 ataaaaattg gcctcgcgtt gatttttttc aaaaaagtgt tggcgtgccg tcgctgtggg
     5401 attttcaatg aggcggagaa tttcttctgt ggcggtatgt ttttcccagt tgaaccacgc
     5461 gttgccgttg cattcttttt tgagaatacg aaaaatagcg taatcgcgta cccactcttg
     5521 gtttttgaaa ttgtgcggta cgtcttccat tgcagcgtcg ggcgcagcgt tgtaatgttc
     5581 ttgtaaaata tcttccttaa agcgcacgat gtcataaaaa ataacgcgag aaaatgcgcg
     5641 ggtgcgcttt ttctctgcct ccacgcgttg gcgtaaatcg tcgcagagcg ggaaattatc
     5701 gagcgataag tacacggggt gtagcgccat cgcgctgagc gccgaatagg ggctgctgtt
     5761 gtaaccggtg tcgttcaccg gcaacaaatt gatgatgcgt tgaccggtgg ccacgcacca
     5821 atcgatgagg atcttcaaat cgtaaaactc gccgatagcg caactttgtt gtgatctgag
     5881 cgcacaaact ggcgccgcca ccccagctaa gtaatcgccg atgcgtggat agttatccct
     5941 tacggtttca gcgcgggtgg taaaatgcgt taacacgcaa cttgcgtacg gctgcaaaaa
     6001 gaatactgct cccttctcga cacattcttt cttggttcgg atttcttctt gattagcaat
     6061 tatatttttg taaacgagta agtccgattt ttcgtttaaa tcaaaaaaat ataaaaagac
     6121 atccgacgaa aaatgaacgc gttgttcctg cgcagaatta ttgatcatca ccaggtaacg
     6181 tttgctcacg ccggtggtgt acacaaaaac agcgtcgctt ttttcaacgc gcggaaaata
     6241 taaccgcgtt acgtcgg
//
LOCUS       DAOMPD010000100         6233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5351_length_6233_cov_2.663645, whole genome shotgun sequence.
ACCESSION   DAOMPD010000100 DAOMPD010000000
VERSION     DAOMPD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5351_length_6233_cov_2.663645"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..841
                     /locus_tag="PLY93_04775"
     CDS             188..841
                     /locus_tag="PLY93_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRP68824.1"
                     /translation="MEFIMSALDVVLHLERYLDAWALAYGVWMYAILFLIIFAETGLV
                     VTPFLPGDSLLFAVGAICARGVLSLPIILIGLSIAAIIGDTLNYWVGRYFGDFVTRRF
                     SRIVKPEHIEKTHTFYEKYGAKTIVLARFVPIIRTLAPFVAGIGQMHYRQFAFYNIMG
                     GILWIFSMTIAGYLFGNLDFIRNNFSLVVVGIVFISILPMLIGYIRHFLIKKNKENA"
     gene            838..1506
                     /locus_tag="PLY93_04780"
     CDS             838..1506
                     /locus_tag="PLY93_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRP68825.1"
                     /translation="MKKSQQRLKTHRALMVSAIRLMSDDRSFSSISLREVTREAGVVP
                     ATFYRHFNEMEDLAVELVDYCMRPMENLMTSVRARRLNARQLVYETVSSFLRFARRHR
                     RYSLFLTKERLGGRARVRERIRLSMNNMALMLAHDLKGLPEWRGVPNTVVQDVARMII
                     NQMLVELELVLDLQPSEDLGKDKKEIKIIAGARKRIWILLNGGLSFRERRLKKARLRQ
                     RTNA"
     gene            complement(1496..1909)
                     /gene="atpC"
                     /locus_tag="PLY93_04785"
     CDS             complement(1496..1909)
                     /gene="atpC"
                     /locus_tag="PLY93_04785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRP68826.1"
                     /translation="MAETSQPLTVSIYSPEKMLLEETAVSVGLPGKKGNFSVLKDHTN
                     LVAELEAGVVRVQTGTREEIYVVDSGFAEVQKNKVIVLVEGARLPKDLDAAKEVAALE
                     EALNRKTANAVQYAKKQRDIALRRVRVRVADSLRH"
     gene            complement(1945..3342)
                     /gene="atpD"
                     /locus_tag="PLY93_04790"
     CDS             complement(1945..3342)
                     /gene="atpD"
                     /locus_tag="PLY93_04790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRP68827.1"
                     /translation="MASGKVSQVIGSVLDAKFPEDKLPEIYNALTLTVQGQKLVCEVQ
                     QHLGGGAVRAVALGSTDGVARGQEIQDTGAPISIPVGLKSLGRIFNVLGEPVDEAGPV
                     EVEERRPIHRPAPGFEDIEPKTQIFETGIKVIDLLEPYIRGGKTGLFGGAGVGKTVII
                     QELINNIAKQHGGYSVFAGVGERTREGTDLFLEMQESGVIDKTCLVYGQMNEPPGARL
                     RVALTALTQAEWLRDYAGTDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATE
                     MGQLQERITSTKKGAITSVQAIYVPADDITDPAPATAFTHIDASTVLSRKISEKGIYP
                     AVDPLGSTSRALSPGVVSDRHYKIAREVQRILQRYKDLQDIIAILGMDELSEDDKVLV
                     GRARRIERFLSQPFHVAEQFTGVPGKYVKLEDTIRSFEELLSGKYDKYPEGAFYMCGA
                     IEDVIEKAKAMDGQG"
     gene            complement(3357..4223)
                     /gene="atpG"
                     /locus_tag="PLY93_04795"
     CDS             complement(3357..4223)
                     /gene="atpG"
                     /locus_tag="PLY93_04795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRP68828.1"
                     /translation="MATAKEIRNRIGSVQNTKKITRTMELVSTAKAKKAVDKVVASRP
                     YADKIKELIGSLASQGDVADHPLLRRHTQVRKALVVVVTANRGLCGGYNTNAIRMAIH
                     RIEEYRKSGVEVDIHLIGKKAINYFNFQKIRYAASFTNIDDKMTFEDAQAFAEKYMES
                     FSLEAVDKIDIVYTKYLSAATQKPVVETVLPVELSAEKEGAAVSPFIFEPDPQGILSS
                     LLPKAITMSVYQSLLDAVASEQIARRIAMKSATDAASDMIKSLTLQYNRIRQAKITQE
                     IAEIVGGAAAIQ"
     gene            complement(4269..5789)
                     /gene="atpA"
                     /locus_tag="PLY93_04800"
     CDS             complement(4269..5789)
                     /gene="atpA"
                     /locus_tag="PLY93_04800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRP68829.1"
                     /translation="MKINTAEIVSVLKSEIQNYGQNLTIDEVGQVLEVGDGIARVYGL
                     SNCLAGEMLEFENGVTGLAFNLEENSVGVVILGDYLKIKEGFSIKRTGKILQVPTGDA
                     LIGRVVDPLGNPLDGRGPINAKSTRPVEIIAPGIARRQPVSEPLQTGIKAIDAMIPIG
                     RGQRELIIGDRSTGKTAIAIDTIINQKGTGVICVYVAVGQKSSTVAGVIDKLKEHGAM
                     DYTIVVAANASDPAPLQYIAPYSGTAMAEEFMYNQGKATLCVYDDLSKQASAYRQMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDKYGGGSLTALPVIETQEGEAYIPTNVI
                     SITDGQIYLQPNMFAAGIRPAVDVGISVSRVGSAAQIKAMKKVAGTMRLDLAQFRELE
                     AFAALGTELDAVTQRQIDRGQRLVELLKQPQYKPMPVEKQVLSIYAATKGFMDKIPVA
                     KIRDFEDRLLEFIEKTHNEFFVAIRDTKSLPDEAKLNTVLADFAESYLRSASPDPR"
     gene            complement(5831..>6233)
                     /locus_tag="PLY93_04805"
     CDS             complement(5831..>6233)
                     /locus_tag="PLY93_04805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRP68830.1"
                     /translation="QPQILSLCLILIEKRKQVLIHDIFIAYRSVLDNILGRTYVEVTV
                     AKELASGGIDDTLKQAIIQKVDTHRAAFGLESAKRLTYEVNVKLNPELLAGVRVRVAD
                     YIFDGTVERNLIHWRDKAVLRPINAQKAFEG"
BASE COUNT         1508 a         1600 c         1399 g         1726 t
ORIGIN      
        1 ctaaaacgca ctcaaattga ccccaaaagt gtcagttatc tctgtgggtt gcataaaaac
       61 tcttttgagc ctgtgagtca acgcgcgcaa cgcgcacaaa aatttgcaaa aaaaagttac
      121 ctcagcgcgt tccagcctga ccggttttgc gattgtcgcg ctgtttccgc ttataccttt
      181 agtaaacatg gagttcatca tgtctgcgct tgacgttgta ctacacctag agcgctatct
      241 cgacgcatgg gctctcgcat acggcgtatg gatgtacgcc attctcttcc ttattatttt
      301 tgctgaaacc ggcctcgtcg tgaccccttt cttgcccggc gactcgttgc tcttcgccgt
      361 cggcgccatt tgtgctcggg gtgttctatc gctccccatt attttaatcg gtctttccat
      421 cgctgccatt ataggcgaca cgctcaatta ttgggtggga cgctattttg gtgactttgt
      481 cacccgtcgg ttttcgcgta tcgtcaaacc agagcatatt gaaaaaactc atacttttta
      541 cgagaaatac ggcgcaaaaa ctatcgtatt ggcgcgcttt gtccccatta ttcgcaccct
      601 cgctcctttc gttgcaggca tcggccaaat gcactaccgc cagtttgctt tttataacat
      661 catggggggg atcttatgga ttttctcgat gactatcgcc ggctatcttt tcggtaattt
      721 agattttatc cgcaacaact ttagtctcgt tgttgtggga attgtcttca tttctatctt
      781 acctatgctc ataggctata tacgccactt tttaatcaaa aaaaataaag aaaacgcatg
      841 aaaaaatcac aacaacgctt aaaaacacac cgcgcgctca tggtgagcgc catccgcctc
      901 atgagcgacg accgctcatt ttccagcatc agtctacgcg aagtcacacg cgaagcggga
      961 gtcgtaccgg cgacgttcta ccgccacttc aatgaaatgg aagatttagc cgttgagctc
     1021 gtcgattact gcatgcgccc catggaaaat ttaatgacga gcgtccgggc gcgccgcctc
     1081 aacgcacgcc agttggttta cgaaactgta agttcttttt tacgctttgc gcgccgccac
     1141 cgccgctact cgcttttctt gacgaaagaa cgtttgggtg gtagagcacg ggtgcgcgaa
     1201 cgcatccgtt tgagcatgaa taatatggct cttatgctcg cacacgatct caagggactg
     1261 cccgaatggc gtggtgtccc caacaccgtc gtacaggatg ttgctcggat gatcatcaac
     1321 caaatgcttg tcgaacttga acttgtgctc gaccttcaac cgagcgaaga cttgggtaaa
     1381 gacaaaaaag aaataaaaat tatcgccggt gcacgcaagc gtatttggat attgctcaat
     1441 ggcgggttat ctttccgcga gcgacgcctt aaaaaagcgc ggctacgcca aaggactaat
     1501 gcctaagtga atcggcaacc cgtacacgca cgcggcgtaa cgcaatgtcg cgttgttttt
     1561 tcgcatactg cacggcattt gccgttttgc ggttgagcgc ttcttcgagc gcagcaactt
     1621 cttttgctgc gtctaaatct ttgggtaatc tagccccttc cacaagcaca atcactttgt
     1681 ttttttgtac ctctgcaaaa cctgagtcga ccacataaat ttcttcgcgt gtgccggtct
     1741 gcacccgaac aacacctgct tcgagctcgg cgaccaagtt tgtgtggtct tttagtacgc
     1801 taaaatttcc ctttttaccg ggtaggccaa cagaaacggc agtttcttcg agcaacattt
     1861 tttcaggcga gtaaatactc acggtgagtg gttgtgatgt ttctgccatt ctttttgttc
     1921 cttaattttt atccctggct atcgctaccc ttggccatcc atggccttgg ccttttctat
     1981 aacatcctca atcgcgccgc acatgtagaa ggcgccttcg gggtatttgt cgtattttcc
     2041 tgaaagtaat tcttcaaacg aacggatggt atcttcgagt ttcacgtatt ttcccggtac
     2101 tccggtaaac tgctctgcga cgtggaatgg ctgtgagaga aagcgctcaa tacgacgcgc
     2161 acgacccacg agtactttat cgtcttctga aagttcatcc attcccaaaa ttgcaatgat
     2221 gtcttgcaag tccttgtaac gctgtaaaat ccgttgtact tcacgcgcaa ttttgtaatg
     2281 gcgatcgctc accactccag gcgacaaagc gcggctggtt gatcccaaag ggtccaccgc
     2341 aggataaatt cctttttctg aaattttacg agaaagaacg gttgatgcgt cgatgtgcgt
     2401 aaacgcggtt gccggcgctg ggtcggtgat gtcgtcggca ggaacataaa ttgcctgtac
     2461 cgaagtaatc gccccttttt tagtcgaagt aatccgttct tgcaactgcc ccatttcggt
     2521 tgcgagcgtc ggttgataac ccaccgcaga gggcatccgg cctaagagag ctgaaacttc
     2581 cgaacccgcc tgagtaaaac ggaaaatgtt gtccacgaag agaagaacgt cggttcccgc
     2641 ataatcgcgc agccattctg cttgagtgag cgcggtcaac gctacacgca aacgcgcccc
     2701 ggggggttcg ttcatctgac cgtacacaag acaagtctta tcgatgacgc ccgactcctg
     2761 catttcaaga aaaaggtcag tcccttcacg cgtgcgctca cccacaccag cgaacaccga
     2821 gtaccccccg tgctgcttcg cgatgttgtt gatgagttct tggatgataa cggttttacc
     2881 cacacccgca ccgccgaaaa gaccggtctt accaccacgg atataaggtt cgagaaggtc
     2941 gatgacttta ataccggttt caaaaatttg agttttaggt tcaatatcct caaagccggg
     3001 agccggacga tggatagggc ggcgctcttc gacttctaca ggacctgctt cgtcgacggg
     3061 ttcgcccaaa acgttaaaaa tacgacctaa cgatttaaga ccaacgggaa ttgaaatcgg
     3121 agcgcctgtg tcttggattt cttgaccacg cgccacacca tcggtagagc ctaacgccac
     3181 cgcacgcaca gccccaccac ccaaatgctg ctgcacttcg cacaccaact tttggccttg
     3241 cacggtaagc gttaacgcat tataaatttc gggaagctta tcctcgggga attttgcatc
     3301 gagtaccgac ccgattactt gactgacttt gcctgacgcc atttttctct ccttaattac
     3361 tgtatcgcag cggcaccgcc gacaatctcc gcaatttctt gcgtaatttt tgcctgacga
     3421 atacggttat attgcaatgt gagcgactta atcatatcgg aagctgcatc ggttgcgctt
     3481 ttcatagcta tgcgccgtgc aatttgttct gaagcaaccg catcgagtaa cgattgatac
     3541 acgctcatgg tgatcgcttt aggtaaaagc gaagataaaa taccctgtgg atcgggctca
     3601 aaaataaagg gagaaaccgc agccccttct ttttctgccg acagttcaac gggcaacact
     3661 gtctcaacga ctggtttttg cgtcgctgca ctcaagtact ttgtataaac aatatcaatt
     3721 ttatcgacgg cttcgagtga aaagctttcc atatattttt ctgcaaacgc ttgcgcatct
     3781 tcaaacgtca ttttatcgtc gatgttagta aacgatgcag cgtatctaat tttctgaaaa
     3841 ttaaaatagt tgatggcttt tttaccgatt aaatggatat ccacctcaac accacttttc
     3901 cgatactctt cgattcggtg gattgccata cggattgcat tggtgttgta accgccgcaa
     3961 agccctctat ttgcagtgac gacgacaacc aatgctttac gtacctgcgt atggcgccgg
     4021 agaagaggat gatctgccac gtcaccttgg ctggcgagcg aaccgatgag ttcttttatc
     4081 ttatccgcat aaggacgcga ggcaacgact ttatcgacag ctttcttcgc tttcgctgtc
     4141 gacacaagtt ccatcgtacg cgtaatcttc tttgtgttct gcacagagcc aatacggtta
     4201 cgaatttctt tcgcggttgc cattttcctc ttatccttct ttaattaatt tcttctatac
     4261 tctttctctt aacgaggatc gggtgatgca ctacgcaaat acgactcagc aaaatcggca
     4321 agtactgtgt taagttttgc ctcgtcgggt aagctttttg tatcgcggat tgcaacaaaa
     4381 aactcgttat gtgttttttc gataaattcc agaagtctat cttcaaaatc ccgaattttt
     4441 gccacgggga ttttatccat aaaacctttc gttgccgcat aaatagagag aacttgcttt
     4501 tcaaccggca ttggcttgta ttgtggttgt ttcaacaact caacaagacg ttggccacgg
     4561 tcgatttgcc gctgcgtcac tgcgtcgagc tcggttccta acgcggcaaa cgcttccaat
     4621 tcacggaatt gcgccaagtc aagacgcatg gtaccggcga cttttttcat cgctttaatc
     4681 tgcgctgcgc tacccacgcg tgaaacagaa atacccacgt ccaccgcagg acgaatgccc
     4741 gcagcaaaca tattgggctg caagtaaatt tgaccatcgg taatagaaat tacgtttgtg
     4801 ggaatgtacg cctcgccttc ttgagtttca ataacaggaa gcgcagtgag cgaacctccg
     4861 ccgtatttat cgctcaactt cgccgcacgc tccaaaagcc gtgagtggag atagaacacg
     4921 tcacccggat acgcttcacg accgggagga cggcgcaata aaagcgacat ttgtcggtat
     4981 gcacttgctt gcttcgacaa gtcgtcgtac acacaaagcg ttgctttgcc ttggttatac
     5041 ataaattctt cggccatcgc tgtgcctgaa taaggagcga tgtattggag tggtgcggga
     5101 tcggacgcgt tcgccgccac aacaatggta tagtccatcg caccatgttc tttgagtttg
     5161 tcgatcacgc cagcgacggt cgatgatttt tgccccaccg ctacataaac gcagataacg
     5221 cccgtacctt tttggttgat gattgtatcg attgcaatcg ccgttttacc cgttgaacgg
     5281 tcgccgatga tgagctcgcg ttggccacgc ccgatgggga tcatcgcgtc gatggcttta
     5341 ataccggttt gtagaggctc ggacaccggt tggcggcggg caataccggg agcgataatt
     5401 tcaacagggc gcgtcgattt tgcattgata gggccacggc catcgagtgg gttaccgagt
     5461 ggatcgacaa cgcgaccaat caaggcatcg ccagtgggga cttgcaaaat cttgccggtg
     5521 cgcttaatag aaaaaccttc tttaattttg aggtaatccc ccaaaataac gacgcccacc
     5581 gaattttctt cgaggttaaa cgctaagccg gtgacgccat tttcaaattc aagcatctcg
     5641 cctgcgagac agttagataa accataaaca cgagcgatac catcgcccac ctcgagcact
     5701 tgtcctactt cgtcgatggt taaattctgt ccgtagtttt gaatttcgct tttgagcact
     5761 gaaacaattt ctgctgtatt aattttcata tcccctcgtt atcgttatcc ttgaacgccc
     5821 tattaaatac ctatccttca aatgcctttt gggcgttgat gggtcgtaag accgccttgt
     5881 cacgccaatg gattaaattt cgttcaacag tgccgtcaaa aatataatcg gcgactcgaa
     5941 ctcgcacccc tgctaataat tcagggttta attttacgtt gacttcatac gttaatcgtt
     6001 tggcgctctc cagtccaaac gctgcacgat gtgtatccac tttttgaata attgcttgtt
     6061 taagcgtgtc gtcgatgccc ccgctcgcta attcttttgc aactgtaact tcaacgtatg
     6121 tacgtcccag tatattatca agcactgaac ggtaggcgat aaagatgtcg tggattaaaa
     6181 cttgttttcg cttttcaatt aaaatgaggc aaaggctcaa aatttgcggt tga
//
LOCUS       DAOMPD010000101         6230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5360_length_6230_cov_2.988988, whole genome shotgun sequence.
ACCESSION   DAOMPD010000101 DAOMPD010000000
VERSION     DAOMPD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5360_length_6230_cov_2.988988"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLY93_04810"
     CDS             <1..407
                     /locus_tag="PLY93_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68831.1"
                     /translation="ARAAGATSFLLIAAALTDAELTAFIAHGRAWGMEPLVEVLTQEE
                     LERVLRLDVKILGINNRNLHTLDIDMTRAVRLAESIPEARRNKLILVAESGYKSRDEL
                     LALPHYFDAVLMGTGFMQESDVAAKLAEVFER"
     gene            complement(539..1225)
                     /gene="uppS"
                     /locus_tag="PLY93_04815"
     CDS             complement(539..1225)
                     /gene="uppS"
                     /locus_tag="PLY93_04815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRP68832.1"
                     /translation="MLHIAVIMDGNGRWALSRGQKRSAGHRAGTETIERLLDHLLKIK
                     IPCVSLYAFSTENWKRPHNEITAIFKLLDEFIETRLQKLIDQGVKIVTSGDISRLPKK
                     SRGLLNDAVLKTKRGKKLTANFCLNYGARDEIHRAALLYATTSPKAKSGKLPTEKEFS
                     RYLWQPRLPDVDLLIRTAGEFRLSNFMLYQSAYAELYFTDKTWPEFTEQDLDAAIAEF
                     KRRKRKFGGL"
     gene            complement(1225..1743)
                     /gene="frr"
                     /locus_tag="PLY93_04820"
     CDS             complement(1225..1743)
                     /gene="frr"
                     /locus_tag="PLY93_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRP68833.1"
                     /translation="MQKTLDDLAHHMAQIRSGRATPSLVEDIRVEVYGQVMPLSQVAS
                     IATPEARLITVDVWDKSQIQTVEKAIMKSGKNLNPSSDGSLIRINLAEMTGETRKEMV
                     KLAKAKTEDHKVAIRNVRRDANDALKKLKGSGVSEDQIKGFSDAVQKHTDGFIKRMDD
                     LFAAKEKDIMTV"
     gene            complement(1781..2530)
                     /gene="pyrH"
                     /locus_tag="PLY93_04825"
     CDS             complement(1781..2530)
                     /gene="pyrH"
                     /locus_tag="PLY93_04825"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRP68834.1"
                     /translation="MVEGRAYRRVLLKLSGEVLAGDDKQGINVKVIHALAEEIREIHR
                     ELGGIELAIVIGGGNIFRGVSAASDGMDRAIADYMGMMATVINGLAMQDFLEKEGLVT
                     RLQTAIEIKSVAETFIRRKAIRHLEKKRVVILSGGTGNPYFTTDTTAALRAVELDADV
                     IFKATKVDGVYDSDPIKNKKAKRYRRLTFKDALVNQLKVMDSTAFSLCLDNKMPIQVF
                     DMAKEGNLMRAIKGEAIGTLIQAEGESEFYE"
     gene            complement(2562..3152)
                     /gene="tsf"
                     /locus_tag="PLY93_04830"
     CDS             complement(2562..3152)
                     /gene="tsf"
                     /locus_tag="PLY93_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRP68835.1"
                     /translation="MAIDQEKIKALREMTSAGILDCKKVLEETNGELEKAASLLRERG
                     IVKAVKKMDREATEGAIVSYVHHNGKIGVLVQLNCETDFVARNEKFIELGKNIAMHIA
                     ATNPLFVKVEDVSPDVLEKEREVQRAALIEEGKPADKVDKILEGKLKKFASDICLLEQ
                     AFVKEPDKTVSDIIKAEIATLGENISVGRFVRYALK"
     gene            complement(3160..>3373)
                     /locus_tag="PLY93_04835"
     CDS             complement(3160..>3373)
                     /locus_tag="PLY93_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRP68836.1"
                     /translation="QYPIPANDDAIRAVALFLEIMANAIEEGKEGGEFQQVEIEEESM
                     DEDQLRSSMEKLDDLEEIESRYEERA"
     assembly_gap    3374..3383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3384..3928)
                     /gene="rpsB"
                     /locus_tag="PLY93_04840"
     CDS             complement(<3384..3928)
                     /gene="rpsB"
                     /locus_tag="PLY93_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRP68837.1"
                     /translation="MATISMKSLLEAGVHFGHQTRRWNPKMKKYIFTARNGIHILNLQ
                     KTIVLAKDAYEAMRDLASKGGKVLFVGTKKQAQADIEKSAKRCGMPYINNRWLGGLLT
                     NFRTVKQSIQRLRKLEDAFENNTVHEIVKTKKEILQLNRELARLRKDFSGIRDMNDLP
                     DALFVVDPDRETIAVKEANTLG"
     gene            complement(3959..4108)
                     /locus_tag="PLY93_04845"
     CDS             complement(3959..4108)
                     /locus_tag="PLY93_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68838.1"
                     /translation="MQKNCFLVHAARAGAQELKEIDTLSQKEIFTITGKSPVLPVADF
                     LFRDL"
     gene            4341..5018
                     /locus_tag="PLY93_04850"
     CDS             4341..5018
                     /locus_tag="PLY93_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HRP68839.1"
                     /translation="MKTMVRQARQSKTLILILVMAILGCDGYGQYFVEDEPTTNPPTS
                     SEPIQPVFISKWDTTQTGASASNQIRLPLNASGSYNFTVDWGDGSANSIASAGDPAKV
                     HTYASGGIYTIKITGQIDGFGFANSGEDNKKLLDISQWGQVKLHNSGYVFYGITLVSF
                     SASDSPDLSNVTNMDSMFYNASAFNQDLSAWGDAGGGALCTNIATKPTNFNSGAGTIT
                     LPCGWQP"
     gene            5139..5759
                     /locus_tag="PLY93_04855"
     CDS             5139..5759
                     /locus_tag="PLY93_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP68840.1"
                     /translation="MHSFNSYLAQGIGGLPSSLISIILIIVSILMLTIALERGYILFR
                     LNTPINHNQARDFLHLIREKKFEEAKAYCADKAHPGFRTMQRILENRTSTTDILTLAK
                     EEILIEQTALERFTSFLGTTTTLAPMLGLLGTVTGMIKAFSAFAQASSHSGQLTIGIH
                     EALITTTLGLMIAIPALLFFNFYVKRTNLLMDECEHLIEQSLKELL"
     gene            5759..6157
                     /locus_tag="PLY93_04860"
     CDS             5759..6157
                     /locus_tag="PLY93_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP68841.1"
                     /translation="MLRTRKRLSLRTGIELAPFVDILFLLVTYFLMNATLANNPAIKI
                     ELPKSDTSIASETQPLVVLINQDNQIYVNERNVPLKDLGKTVVGLLKDKEKDQVIIRG
                     DKRSSYQIVISVMDELNKAGVTRFNLATDK"
BASE COUNT         1504 a         1489 c         1427 g         1800 t
ORIGIN      
        1 aagcgcgtgc agccggtgca acgagttttt tactcatcgc tgctgcgctc accgacgccg
       61 agcttacagc gtttattgcg catggccgtg cttggggtat ggagccgctc gttgaagtgt
      121 tgacccaaga agaattagag cgcgttcttc gccttgacgt taaaattttg gggatcaaca
      181 accgtaacct acacactctc gatatagaca tgacgcgtgc ggtacgcctt gcggaaagta
      241 ttcccgaagc gcggcgtaac aaactcatcc tcgtcgccga atcgggttac aaaagccgcg
      301 acgaactttt agccttaccg cactacttcg acgcggtact catgggcacg gggtttatgc
      361 aagaaagcga tgtggcggca aaattagccg aagtgtttga gcgctaattt tttatgtcct
      421 gtaatatccc aacgccgtaa ttttgtttta gtgtattact cctttaattg gtgagccgtt
      481 cttaaagata cgcgaatatc tttggagtgc gtaaacttat ctccgcctac gaacaaagtt
      541 acaatccacc aaacttgcgt ttgcgccgct tgaactcggc aatcgctgcg tctagatctt
      601 gttcggtaaa ttctggccaa gttttgtcgg taaaataaag ttcggcgtat gcggattggt
      661 agagcataaa attagacagc ctaaattcgc ctgcagtgcg gatgagtaaa tcgacgtcgg
      721 gtaaacgggg ttgccaaaga taacgactga attctttttc ggtgggcagc ttcccgctct
      781 ttgctttcgg cgatgtagtg gcgtagagta aagcggcgcg gtggatttca tcgcgcgcac
      841 cgtagttgag gcaaaaattg gcggtgagtt ttttgccgcg ctttgtttta aggacggcat
      901 cgttcaatag cccacggctt tttttaggca accgtgatat atcccctgag gtaacgattt
      961 taactccttg gtcgatcaat ttctgcaagc gggtttctat aaactcgtcg agcagtttga
     1021 aaatcgccgt gatttcgttg tgcggtcttt tccaattttc agtggaaaat gcatagaggc
     1081 ttacacaggg aatttttatt ttaagaagat gatcgagcaa tcgttcgata gtttcagtgc
     1141 ctgcgcggtg cccagcgctg cgcttttggc cgcgcgaaag tgcccagcgg ccattgccat
     1201 ccataatgac cgcgatatgg agcatcaaac cgtcatgata tctttttctt ttgcggcaaa
     1261 aaggtcgtcc attcttttga taaaaccgtc ggtatgtttt tgaaccgcgt cggaaaagcc
     1321 ttttatttgg tcttcagaga caccgctacc tttaagtttc ttgagtgcat cgttggcgtc
     1381 gcggcgtaca ttgcgtatcg caactttgtg gtcttcggtt ttggcttttg ccaacttcac
     1441 catttctttg cgcgtttcgc cggtcatttc ggcaaggtta atgcggatga gcgaaccgtc
     1501 gctcgaaggg ttcaagttct tgccggattt cataatcgcc ttttcgaccg tttgtatttg
     1561 cgatttatcc catacatcga cagtgataag ccgcgcttcg ggggtggcaa tcgacgctac
     1621 ttggctaagc ggcatcactt ggccatagac ttcaactcga atatcctcca ctaacgaagg
     1681 cgtcgcacgc cccgaacgga tttgcgccat gtggtgcgcc aaatcatcga gtgttttttg
     1741 catgcgattt ttcgcatcgt caataatttt gtttgcgtca tcactcataa aattctgact
     1801 ctccttcggc ttggatgagc gtaccgattg cttcaccttt gatggcgcgc atcaggttcc
     1861 cttctttcgc catgtcaaag acctgaatgg gcatcttgtt gtcgaggcaa agcgaaaatg
     1921 cggttgaatc catgacttta agctggttga cgagcgcatc tttgaaagta agcctgcggt
     1981 agcgttttgc ttttttattt ttaatagggt cagaatcgta cacaccatca actttagtcg
     2041 ctttaaaaat gacatctgca tcgagttcga ccgcacgcaa cgctgctgtt gtgtcggttg
     2101 taaaataggg atttcctgtg ccaccggata aaatcaccac acgctttttt tctaagtggc
     2161 ggatggcttt gcgccggata aaagtttcag cgaccgattt aatctcaatc gctgtttgca
     2221 aacgcgtgac tagtccttct ttttcaagaa agtcctgcat tgccaagcca ttgataaccg
     2281 tcgccatcat gcccatataa tcggcaatgg cgcgatccat accatcggac gcggcagaga
     2341 caccacgaaa aatattaccg ccaccgataa caatcgccag ttcaataccg cccagttcac
     2401 ggtggatctc acgaatctcc tcggcgagtg catggatgac tttgacattg atgccttgct
     2461 tatcgtcgcc ggctaaaact tcacccgaaa gttttaaaag cacacggcga tacgcccgcc
     2521 cttcgaccat tccgacggca gagtcaagcg tagcgttcat gttatttcaa tgcatagcgg
     2581 acgaaacgtc cgacggagat attttcaccc agcgtcgcaa tctcagcctt gataatgtcg
     2641 ctgacggttt tatctggttc tttgacaaac gcctgctcca aaagacaaat atcggaagca
     2701 aatttcttga gtttaccttc caaaatttta tcgactttat cggctggctt accttcttcg
     2761 atgagcgctg cacgttgtac ttcacgctcc ttttcaagaa cgtcggggga aacgtcttcg
     2821 acttttacaa aaagtgggtt tgttgctgca atatgcatcg cgatattttt acccaactcg
     2881 ataaattttt cgttacgcgc cacaaaatcg gtttcgcaat taagttgtac caaaacgcca
     2941 atttttccgt tatgatggac atacgataca attgcgcctt cggtagcttc gcgatccatt
     3001 tttttgactg ccttcacaat gccgcgttcg cgtaaaagcg acgcagcttt ttccagctcg
     3061 ccgtttgttt cttctaatac ttttttacaa tcgagaatac ccgctgaggt catctcgcgc
     3121 agtgctttaa ttttttcttg gtcgattgcc ataattcccc tatgcccgtt cttcgtagcg
     3181 cgattcgatt tcttccagat cgtctaattt ttccatagaa gaacgtaact ggtcttcatc
     3241 catgctttct tcttcgattt caacttgctg gaactcaccg ccctctttgc cttcttcaat
     3301 cgcgtttgcc ataatctcta aaaagagtgc aaccgcgcgg atggcgtcgt cgttggcggg
     3361 gatggggtat tggnnnnnnn nnncccaatg tgttggcttc tttaacggca atcgtttcgc
     3421 ggtcggggtc gacgacaaaa agcgcatcgg gtaaatcgtt catgtcgcgg atacccgaaa
     3481 agtctttgcg caagcgcgct aactcgcggt tgagttgcaa aatttctttt ttagttttaa
     3541 caatttcgtg gacggtgttg ttctcaaacg cgtcttcgag cttgcgtaac cgttggatcg
     3601 attgcttcac cgtacgaaaa ttggtgagta aaccacccaa ccagcggttg ttgatatacg
     3661 gcatcccgca acgcttcgct gatttttcaa tatcggcttg cgcttgtttt ttggtgccga
     3721 caaaaagaac tttaccgcct ttagatgcaa ggtcgcgcat cgcttcgtac gcatctttgg
     3781 cgagcacaat cgtcttttgc aaattcaaaa tgtggatgcc gttacgcgcg gtgaagatgt
     3841 acttcttcat ttttgggttc caacggcggg tttggtggcc gaagtgaacg cctgcctcta
     3901 agaggctttt cattgaaatg gttgccatat tttctcctag gggattctct tatatcccct
     3961 ataaatccct gaacaaaaag tccgccactg gtagcacggg agattttcca gtgatggtaa
     4021 agatttcttt ttgagacaac gtgtcaattt ctttgagttc ctgtgcgcct gcccgcgcag
     4081 cgtgtacaag gaagcaattt ttttgcaagc gtttgtgcac gctgcgcgcg caggctcccc
     4141 gccgacagtc agtttttccc attgacagag ccatttagat ttggtatttc tgcccgcaat
     4201 gcaaagcttg aagtttttat tttactgtct cgtgctttgt atttctagcg cggcgccaac
     4261 gatgaacttg tcgagcgcgt ctataggagc aggctacagg ttttaaagaa tgaacaatct
     4321 aaaaaatata ggagtataaa atgaaaacaa tggttcgaca ggctcgccaa tcaaaaacac
     4381 taatcttgat attagtcatg gctatactgg gctgcgatgg ttacggtcaa tattttgttg
     4441 aagatgagcc caccaccaac ccacctactt ctagtgaacc aatccaaccc gtttttatca
     4501 gcaagtggga taccactcaa acaggcgctt ctgcctcaaa ccaaattcgg ctacccttaa
     4561 atgcaagtgg tagttataac tttactgtcg actggggcga tggcagcgct aattcaatcg
     4621 cctctgctgg tgaccctgcg aaagtgcata cttatgccag tggcggcatc tacacgatca
     4681 agataaccgg tcaaattgat ggctttggtt ttgccaattc aggtgaagac aataagaagt
     4741 tgctcgacat tagccaatgg ggccaagtga agttgcataa ctcgggttat gttttttatg
     4801 gcataactct ggtgagcttt agcgccagtg atagccccga tttaagcaat gtgaccaata
     4861 tggatagtat gttttacaac gctagtgcct ttaatcaaga cttgagcgct tggggtgatg
     4921 ctggtggtgg cgcattgtgt acaaatatag caacaaaacc caccaacttt aattctggtg
     4981 cgggtactat aactctgcct tgtggctggc aaccctaaaa ctggaatacc gcatcggggc
     5041 agaaaagggg cgcggtattt tggctgcgcg agttgcgcat gggttcttta tccactttaa
     5101 gaggttgtcg gtaagagtcg aaaatttgaa attgtggtat gcatagcttt aactcgtatc
     5161 tggcgcaagg cattggcggt ctgcccagtt cactcatttc aattatcctc attatcgtat
     5221 cgattttaat gctcacgatc gcgttagaac gaggatacat tcttttccgt ttgaacaccc
     5281 cgattaacca caatcaagcg cgcgattttt tacacctcat ccgcgagaaa aaatttgagg
     5341 aagctaaagc gtactgcgcc gacaaagcgc atcccgggtt tcgcacgatg cagcgcattt
     5401 tagagaaccg tacttcaacc accgacattc taacgttagc aaaagaagaa atacttatcg
     5461 aacaaactgc gctcgaacgc ttcacttcct ttttaggcac cacaacgaca ctcgcgccga
     5521 tgttgggtct tttgggtacc gttaccggta tgatcaaagc gtttagcgct tttgcacagg
     5581 cgtccagtca ttcggggcag ctcaccatcg gtatccacga agcgctcatt acgacgacgt
     5641 tggggcttat gatagctatc cctgcgttgc tttttttcaa cttttatgtg aagcgtacca
     5701 acctcctcat ggacgagtgc gaacatctca tcgagcagtc tttaaaagaa ctcctctaat
     5761 gctacgcaca cgcaaacgtc tttccttacg aaccggtatc gagcttgcgc cgttcgtcga
     5821 tatacttttt ttattggtca catatttttt gatgaacgca acgctcgcca ataaccctgc
     5881 gattaaaata gaactcccaa aatccgacac gtcgattgct tccgagacac aacctttagt
     5941 cgtgctcatt aaccaagata accagattta tgtcaacgag cgcaacgtac cgctcaaaga
     6001 tttggggaaa accgtcgtgg gattattaaa agacaaagaa aaagatcaag taattatacg
     6061 cggcgataag cgttcatcgt accaaatcgt tatttctgtt atggacgaac tcaataaagc
     6121 cggggttact cgctttaacc ttgccaccga caaatagatg aagttgcgct taccactgag
     6181 cgctcaagat attttttttc ggtgcgccgc tttcttgtgg ggatttactt
//
LOCUS       DAOMPD010000102         6217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5368_length_6217_cov_3.270691, whole genome shotgun sequence.
ACCESSION   DAOMPD010000102 DAOMPD010000000
VERSION     DAOMPD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5368_length_6217_cov_3.270691"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..954
                     /locus_tag="PLY93_04865"
     CDS             49..954
                     /locus_tag="PLY93_04865"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRP68842.1"
                     /translation="MSAIKSPIPKRLPWSFDEPTELIAYKNVHTVCEESRCPNRNECS
                     SWGVATFLIGGRECTRACKFCHVATGKPIPLSHIVDREHDDIINAAKARDYKYLVITS
                     VARDDDEAGLAAHFARITRSLNALGVTVELLIPDFHARPEHLRVVADAKPAVIAHNVE
                     TVERLSPYIRPQAKYQRSLAFFNFFREAYPHIVRKSGFMVGLGEERSEISTLLSDMKR
                     AAVEIVTVGQYLRPTTKQAPLLKNYPMTEFLEIESEIETYGFLAWEVGPYVRSSYMAE
                     TTMAKLKAELVRRNSHIHDDTLHLK"
     gene            960..1898
                     /locus_tag="PLY93_04870"
     CDS             960..1898
                     /locus_tag="PLY93_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68843.1"
                     /translation="MAVAKGLYIDFHKLELSHLKTLVDENKIDTALVDNLLKYWQRRL
                     DDKKDAIYSRYIEYYLFFESIKALFFAVFIFDMKNGGTRHSYGIFPVLAPLIYSAINS
                     AAKEKSGVLDFDHNGMKYALYFTHSGYHEQEYAVAALALKGTSIDDNLKRLIYVFERY
                     YMPLSFSRDERTSFLFTNTANEIQNLTEKTLKAKKPVTFTYLYFESLTKYVSQSGESF
                     ARELLDELRADVHRILKDIDHSFVLSTREMLVVSLNCETEAMQKRFTNQIFRAKGLLL
                     AVQLKFYTVQEPISDLNLLWNDITGNIAYQRKLKKL"
     gene            complement(1901..2353)
                     /locus_tag="PLY93_04875"
     CDS             complement(1901..2353)
                     /locus_tag="PLY93_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68844.1"
                     /translation="MIFVAFIAPLTAMTLEGRLPGERFLEDNPPRFGAVMSFVSRSHS
                     LVLVDARVEKPNYIIAKLLNKIARSFKINRKELKKVTLVEGEMRWLQYTFRKGAGGGF
                     FYVTRQENVFVYVLIYNFSYDALANDMPFVDRYIQELQLVNDEQGTPP"
     gene            complement(2445..3782)
                     /locus_tag="PLY93_04880"
     CDS             complement(2445..3782)
                     /locus_tag="PLY93_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68845.1"
                     /translation="MKKRNRFIFIFTALLFVGGIFYLNADTSAKKDIRNVSEIFNIDE
                     PYSFIAAVGDVPAFVEALRTGDAMRAFFDSPLGLHFIRSAPTRAAAHLHRYIAMAPQS
                     WQWNLYALFIHGPVFYRSTRSQFVLMVQLNKKGKLVSSLMKLGNAARVGDWLIVASDN
                     AALKAQQDYLQKPQIKNFILDDYLRDKASLSFVVGEQGTTRKKRSLFRALIHEMLGIQ
                     SFNACVFRVRPVANAVTMNGSCPNAAVNTVAKSEILNTPHSPFTAYAFRIGAPKADVV
                     AFSGFTTEYKLIPQLFYSGSTQHKSVLEFLSQAFKTNTHQVHAIENGFQIQYPLAYAY
                     GDKKFYQFSPLLTADNERFYWQSCDLALPKQQVAIDATNQFFVKVAIAPLIKNSLDAI
                     NAIDVIYSPAHFYEFRDALIKSMPSLTDATIQVSAKIQNKGLRIDGTLNFAKQ"
     gene            complement(3779..4255)
                     /locus_tag="PLY93_04885"
     CDS             complement(3779..4255)
                     /locus_tag="PLY93_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68846.1"
                     /translation="MSLTTRKGIILCVDDEPGVLSTLKEQLLADFTTTHEVVTATSGE
                     EALAFVEAARSEEEIIELIITDQMMPSMKGDEFLKRVHSILPDAMKILLTGHAGLDSA
                     IKAINEGGLNRYIEKPWNAEAMRDDIKGLITKFRQNLENQYMLNNLEARIRELTSA"
     gene            complement(4265..4900)
                     /locus_tag="PLY93_04890"
     CDS             complement(4265..4900)
                     /locus_tag="PLY93_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HRP68847.1"
                     /translation="MRLLVVSLLFTSFLFADKDGWKYEKSSGGVTAYTRTIPTSSFKE
                     YKVETEVDATLSQVVAVLMDVPAMPQWVDRCSSASMLKEISSTESITRSITAAPFPLK
                     NREAIAHGKLVQDKITKVVVLTSSGRSSYLPPDPRYERVKHVRGLWVLEPKPNGKVHI
                     KMIGHTDPGGVIPAAIANQFVVTVPFNTIKNLRNQIKKPKYINQKLSYIKN"
     gene            complement(4909..5754)
                     /locus_tag="PLY93_04895"
     CDS             complement(4909..5754)
                     /locus_tag="PLY93_04895"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRP68848.1"
                     /translation="MRKIFIVSILLLFSAQHFAAPKKTKAAEKGVPLKFYNETMNFRI
                     IWKGIAAGDVTLRSRIDDDQFININSRVNSLQSVRGIYYVQGVFASKWNFVTRSPVIA
                     FEEAYQGDSYQRRKFRFIGNEAQVEKHEKKFHEHSYPHSEPPKGDWSDKYSVSTKGGY
                     QDLLGAFYYVRSTGRVPRVGEVMRVPVLPAGSRRSLILKVLGREKRKDVPFFGTREII
                     HVRSALADDAKNKTGKGSNIFFNTKSQIDMWITADEDFVPVKLWTEVPYLGTAYILLE
                     NYSQP"
     gene            5971..>6217
                     /locus_tag="PLY93_04900"
     CDS             5971..>6217
                     /locus_tag="PLY93_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP68849.1"
                     /translation="MNPFPEQNKSWRHVYRWGDTDKKVDPTIQKHIDHFKHVFGIEGD
                     ANDTPLNVNAYVPVKLAKRTKLSRVALRDLMRIVGSEN"
BASE COUNT         1741 a         1435 c         1346 g         1695 t
ORIGIN      
        1 agtcggtctt gccgatcaaa tggagtcggc gtataaattt ttgaatccat gagcgccatt
       61 aaatcaccga ttcctaaacg tttgccatgg tcgttcgacg aacccaccga gctcatcgcg
      121 tataaaaacg tgcacaccgt gtgtgaagag tcgcgttgcc ccaaccgcaa cgaatgctct
      181 tcgtggggcg tcgccacgtt tctcatcggc gggcgcgagt gcacacgcgc gtgtaaattt
      241 tgccacgttg ctacgggtaa acctattccg ctctcacaca tcgtcgacag agaacacgat
      301 gacattatca acgcagcgaa agcgcgggac tataagtatt tagtgatcac ttctgtagcg
      361 cgcgacgacg atgaagcggg acttgctgcg cactttgcac gcatcactcg aagcctcaat
      421 gcgctcggcg tcaccgtcga acttttaatt cccgattttc acgcgcggcc agaacatttg
      481 cgcgtcgtag cagacgcaaa acctgccgtc attgcgcata acgtcgaaac tgtcgaacgg
      541 ctttcaccgt acatccgtcc ccaagcgaag tatcaacgat cgctcgcctt ttttaatttc
      601 tttcgtgagg cgtatccgca cattgtacgc aaaagtggtt tcatggtggg gttaggcgaa
      661 gaacgctcgg agattagcac tttactaagc gatatgaaaa gagcggcggt tgaaattgtc
      721 accgtgggac aatacttacg ccccacaaca aaacaagcgc cgcttttaaa aaattatccc
      781 atgacggaat tcctagaaat tgagtcagaa atcgaaacgt atggcttttt agcgtgggaa
      841 gtgggccctt atgtacgctc cagctacatg gctgaaacaa cgatggcaaa actaaaagcc
      901 gaacttgtac ggcgcaacag ccacatccac gacgatacac tacatttaaa ataaaaacta
      961 tggcggtagc aaaaggatta tacatcgact ttcacaaact cgaactttcg catttgaaaa
     1021 cgttggtaga tgaaaataaa atcgacaccg ccttggttga taatttactg aaatattggc
     1081 aacgccgatt ggacgacaaa aaagacgcta tctattcgcg ctacattgag tactatcttt
     1141 tttttgaatc gattaaagca ttgttttttg ctgtgtttat tttcgatatg aagaatgggg
     1201 gcactcgcca cagttatgga atttttccgg tgttggctcc gcttatctac tcagcgatca
     1261 acagtgctgc aaaggaaaaa tcgggtgtgt tggattttga tcacaacggc atgaagtacg
     1321 cattatattt tacccatagc ggctatcacg agcaagaata cgcggttgcg gcgctggcgc
     1381 ttaaaggcac gtccatcgac gataatctca aacgactcat atacgtgttt gagcgttatt
     1441 atatgccgct cagtttttca cgcgacgagc gcacctcatt tctttttacc aacaccgcca
     1501 acgaaattca aaacctcacc gagaaaacgt tgaaggcaaa aaaaccggtc acttttactt
     1561 atctttattt tgaatcgttg acgaaatacg tgtcgcaatc gggtgaaagt tttgcacgcg
     1621 aattgttgga tgaactgcgc gcggatgtac acagaattct aaaagatata gatcacagtt
     1681 ttgtgctttc aacgcgcgag atgttagtgg tttcactcaa ctgcgaaacc gaagcgatgc
     1741 aaaaacgttt caccaatcaa atttttcgcg caaaaggatt attgctggcg gttcaactca
     1801 aattttatac tgttcaagag cctattagcg acttaaattt actctggaac gatatcaccg
     1861 gcaacattgc gtaccagcga aaattaaaaa aattgtgacg ctacggtggc gttccttgct
     1921 cgtcgttaac taactgcaac tcttgaatat agcgatcgac aaaaggcata tcgtttgcca
     1981 acgcatcata gctaaaatta taaataagaa cgtaaacaaa cacgttttct tggcgcgtca
     2041 cataaaaaaa tccaccaccc gcaccttttc gaaaggtgta ttgcaaccat cgcatttcac
     2101 cttcgacgag cgttactttt tttaactctt tgcgattaat tttgaagctg cgcgcaattt
     2161 tatttaacaa ttttgcgatt atataatttg gtttttcaac acgcgcgtcg accagcacca
     2221 gcgaatgcga gcgagaaaca aaactcatca ctgcgccaaa acgcggtgga ttgtcttcaa
     2281 gaaaccgctc gcctggcaaa cgcccttcga gcgtcattgc tgttaaaggc gcgataaacg
     2341 caacaaagat caacgttatc gcgaaggcac gacaaaccgc gttcgccgtg gaatttgccg
     2401 cgcacgaagt atgggcggtt ttgagatact ctcttatata aaatttattg cttcgcaaaa
     2461 ttaagagtgc cgtcgatacg tagtccctta ttttgaattt tagcagaaac ttgtattgtc
     2521 gcatcggtta aactcggcat cgacttaata agcgcatctc taaattcata aaaatgggcg
     2581 ggcgaataaa ttacatcgat cgcatttatc gcatcgagtg aattttttat caacggggca
     2641 atcgctactt ttacaaaaaa ttgattggta gcatcgatgg cgacctgttg ttttggcagc
     2701 gctaaatcgc atgattgcca ataaaaccgt tcgttatcgg cggtaagtaa tggagaaaat
     2761 tgataaaact ttttatcgcc gtacgcatac gcaagtgggt actgaatttg aaagccattc
     2821 tcgatcgcgt gcacttggtg tgtatttgtc ttaaacgctt ggcttagaaa ctcaagcaca
     2881 gatttatgtt gtgtgctgcc agaataaaaa agctgcggta tcaatttgta ttcggttgta
     2941 aacccgctaa acgcaacgac atctgctttc ggtgcaccga tgcggaacgc gtaagcggtg
     3001 aacggcgaat gcggagtatt taaaatttcg cttttggcaa cagtattcac tgcggcattg
     3061 gggcaactcc cgttcattgt gacggcattt gcaacggggc gtacgcgaaa tacgcaggcg
     3121 ttgaacgatt gaatgcccaa catttcgtgg ataagcgcac gaaaaaggct acgttttttt
     3181 cgagttgtac cctgttcgcc aacgacaaac gaaagtgaag ctttatctct caaataatcg
     3241 tcgagaataa aattttttat ctgcggtttt tgtagataat cttgttgtgc tttaagcgct
     3301 gcgttatctg aggcgacgat aagccaatcg cccacacgcg cggcattccc cagcttcata
     3361 agcgaagaca ccaacttccc tttcttgttt agttggacca tgagcacaaa ttgcgaacgc
     3421 gtggaacgat aaaaaactgg gccatggatg aacagcgcgt ataaattcca ctgccacgat
     3481 tggggcgcca ttgctatata acgatgtaag tgagccgccg cacgcgtggg cgcagagcgg
     3541 ataaaatgca atcccaacgg cgaatcaaaa aaagcacgca tcgcatcgcc agtacgcaat
     3601 gcctcgacaa acgcaggcac atcgcccacc gctgcaataa acgaatacgg ttcgtcgatg
     3661 ttaaaaatct cagaaacgtt acggatgtct ttttttgcag aagtatcggc gttgagataa
     3721 aaaataccac caacaaaaag caaagcggtg aaaataaaaa tgaaacgatt ccgctttttc
     3781 atgcgctcgt taattcgcgg atacgcgctt ccaagttatt taacatatat tggttttcta
     3841 aattttggcg gaactttgtt atgagccctt taatgtcgtc gcgcatagct tctgcattcc
     3901 acggtttttc aatataacga ttaagaccgc cttcgttaat cgctttaatc gccgagtcca
     3961 accccgcatg gccggtcaaa aggattttca ttgcatcggg caaaatagag tgcacccgtt
     4021 tcaaaaattc atcacctttc atgctgggca tcatttggtc tgtaataata agttcgataa
     4081 tttcttcttc cgaacgcgcc gcttcgacaa acgccaacgc ttcttcaccc gaagtcgccg
     4141 tgacaacttc atgtgttgtg gtaaaatcgg cgagaagttg ctctttgagt gtggatagca
     4201 cccctggttc gtcgtcgaca cagagaataa ttcctttacg agtagttaat gacacatttc
     4261 ctccttagtt ttttatataa ctcagttttt gatttatgta tttcggcttt tttatttgat
     4321 tgcgtaaatt ttttattgta ttaaatggta ccgttaccac gaattgattg gcaatcgccg
     4381 caggtatcac tcctcctgga tcggtatgcc caatcatttt aatatgtact ttaccgttcg
     4441 gcttgggttc aagtacccaa agcccccgta catgtttcac tcgttcatac cgtggatcgg
     4501 ggggtaaata tgaactgcgc ccgcttgatg tgagcactac aaccttggtg attttatctt
     4561 gaacgagctt gccgtgcgcg atagcctcgc ggttcttcaa cgggaaagga gcggcggtta
     4621 tagaacgagt gatcgattcg gtggaactaa tttcttttaa catactggcg ctcgaacagc
     4681 gatcgaccca ctgtggcatt gctggtacat ccataagcac cgcaacaact tgcgagagtg
     4741 ttgcatcaac ttccgtttct actttatact ctttaaagct tgatgtggga atcgtacgcg
     4801 tataggcagt cactccccct gaagattttt catatttcca accatcttta tcggcaaata
     4861 aaaaactagt gaataataaa ctaactacta ataaacgcat gcggtaactc aaggttgtga
     4921 ataattctca agtaagatgt acgctgttcc aagataaggt acctctgtcc agagctttac
     4981 cggtacaaaa tcttcatcgg cggtaatcca catgtcgatt tgtgacttcg tattaaagaa
     5041 gatattacta cctttgcctg ttttattttt cgcgtcatcg gctaaagcag aacgaacgtg
     5101 aataatctcg cgtgtgccga aaaaaggcac gtctttacgt ttctcacgcc ccaagacttt
     5161 caaaataaga ctacggcgcg aaccggcggg taacaccggc acccgcatta cttcacctac
     5221 acgaggcacg cggccagtgc tacggacata atagaacgca cccagtaagt cttggtagcc
     5281 accctttgta ctcaccgagt atttatcgct ccagtctccc ttgggtggct cgctgtgtgg
     5341 ataactatgt tcatggaatt ttttttcatg tttttctact tgagcttcgt taccaataaa
     5401 tcgaaattta cggcgttggt atgaatcccc ttgatacgct tcttcaaacg caatcacggg
     5461 gctacgggtc acaaaattcc atttgcttgc aaatacaccc tgaacgtaat aaatccccct
     5521 aaccgattgc aatgagttta cgcgtgaatt tatgttaata aattgatcgt cgtcgatacg
     5581 cgaacgaagc gtgacgtcgc cagcggcaat ccccttccat ataatacgaa agttcatggt
     5641 ttcgttataa aatttaagag gaactccttt ttctgcagcc ttagtttttt taggtgcagc
     5701 gaagtgttgc gccgaaaaca acagaagtat cgacacaata aaaattttac gcattatcac
     5761 taatgtgttt tttgtgttag gggtcaacca taaagacatg tacggctgtg gtaaagttaa
     5821 aagcctgtga ggctgcgcga gttgccgcac agcctcttgt acacttcgtg gtttaacgct
     5881 catttcttga ataattccta cactttaaac aaaaaataag ttcatttacg cttgcccgaa
     5941 tgtcgcaaaa cgcctgaaag ggcgatgctc atgaacccat tccctgaaca aaataaaagc
     6001 tggcggcatg tttaccgctg gggtgacacc gataaaaaag tcgatcccac tatccaaaaa
     6061 catatcgacc atttcaagca tgttttcggc atcgaaggcg atgcgaacga tacgccgctg
     6121 aacgtaaacg cgtacgtgcc cgtaaaactt gccaaacgca cgaagttgag ccgcgttgca
     6181 ctgcgcgact tgatgcgtat tgtggggagt gaaaata
//
LOCUS       DAOMPD010000103         6216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5372_length_6216_cov_2.824379, whole genome shotgun sequence.
ACCESSION   DAOMPD010000103 DAOMPD010000000
VERSION     DAOMPD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_6216_cov_2.824379"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLY93_04905"
     CDS             complement(<1..252)
                     /locus_tag="PLY93_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68850.1"
                     /translation="MAETHITHKNTDDTRPIGRILSGMQATGELHIGHYLGVLTNFAK
                     LQNEAECFYMVANLHSLTTFYTNYHESHKFIAPMISDWLA"
     gene            complement(234..785)
                     /locus_tag="PLY93_04910"
     CDS             complement(234..785)
                     /locus_tag="PLY93_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68851.1"
                     /translation="MNFFHILFDQTGLLFITIPSALIILISRTWFASKMAGEKDAQPL
                     ADFSIASFACLTLTGAAPGGLIQNKPLPLLRFLYGQLWLVFLILMGILYAAIKGPAPD
                     TFAARFSAVFISQAWATFLFNFIPLPPFDAAAGYFSAYTQWRFFAVLVTALTFIVLVI
                     LSFDFWRTNFITGGFLLQWLKLT"
     gene            complement(782..985)
                     /gene="thiS"
                     /locus_tag="PLY93_04915"
     CDS             complement(782..985)
                     /gene="thiS"
                     /locus_tag="PLY93_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRP68852.1"
                     /translation="MIVNGESLSLASVAKPHTLASLITHFSLAPQRVAVELNGALVPR
                     NAYTTTTLNDNDTVELIHYVGGG"
     gene            complement(982..1713)
                     /locus_tag="PLY93_04920"
     CDS             complement(982..1713)
                     /locus_tag="PLY93_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRP68853.1"
                     /translation="MQAHATPLRTLQKKHHLILLSHEEFSFFSEKKMLKLPMRHVEHF
                     HRILRRSTPWSALVSDGGCALVHAQVKDNYLHATDTPIIPILSPPYTVDLVQAWVKPK
                     TLSVILQKAAELGVRRIYFTPSDHSQREQYNEKRCDAILENACMQSYNPLKPTVHILK
                     DLHAIIFPAQDAAFFGDLSATQFFTTIQQEKRTSTLFINGPEGGFSPSEIIFLRTKAT
                     GVLLSENVLRSESAAIIALGYLKAR"
     gene            complement(1720..2574)
                     /locus_tag="PLY93_04925"
     CDS             complement(1720..2574)
                     /locus_tag="PLY93_04925"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRP68854.1"
                     /translation="MRRLRERIQNLNSAYFLLTKIIHKIRLLFILAFIVVLHPPLLHA
                     QGKAGVGGTAFEIMDGVFQQLQYAEGYQSLQLTITTKDGRAVVYKVSLYQKHHASFFV
                     FNSMTRGTVLKLLYNNQGQNIYAYMTHDKRLFHKKYLDRFENILGSGFAYLDIGNGSF
                     VDNYQQKINGVEKNANGEFTVIENLPLEKGNYGKLVVLVDPKKQNRLERIDYYDSAMT
                     LMKTMTFKYGSLPIKETKTSTKQVEYPVRWEMADMSRGTVSTLEFFSIDKTARLDDSL
                     FKKENLEK"
     gene            complement(2583..5351)
                     /locus_tag="PLY93_04930"
     CDS             complement(2583..5351)
                     /locus_tag="PLY93_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRP68855.1"
                     /translation="MSEVETTKEAATAGESDTLEARIQSQFNEEKWTRLSAKDVSISR
                     FKILEQIFDEVKKQNKSGVLRSESLTHLTEYDQSIAAHYFLGMMALEKNIPDEIIHLK
                     TLLDQFQEASKWAVVDYLSEKMLAVTRTRTILRARANALEKLGKHKEAIPVLEQLALL
                     DRKNPDIALKYADAIIAEDLEKGIQFYKQAAEAFARNLQFEKLKTTWNRLIELLPDDF
                     TFYRKIERILAGHRQKETIAELYVLLANYFIKKEDHDKVILLSKKILEYNHNYVRFKN
                     ELLRTYREKYKSHSLLEDFIRYSGLLDSRKSLSSSILNFETNIVFDKGNYVFHRSWGV
                     GKIIDLTTAEMVIDFKDKKAHKMDIQMALKSLKPLREEHFWVMQFEKPDEVKKLFKED
                     ITQFFKILLHSFGGKLSLSDIKTELTDKYVPLTQWSKWWGKTRPELMKDDLIDISPQR
                     KDTIELHDTPVSKAEVVMEKFQAAHGFEDRVLAFVDALKLPTENFEAIEFIAPHFKET
                     LKSLDLNTRLKSLWLLELAAEALADDEPLFAKDQQAEIIAAFQAMNVAELTEFLSEIK
                     QSELKRHGLKFTKRHHAEWRKIYSELLFETPIKLHKTILSELMAAEANTEITDFVNRI
                     RKEAKDNAEVFLWMVKSIVAGSFELSNEQTQESLLGLFRILKSLPKIEQKGSKLKNTA
                     RDIFFGNAKQEIFDTIEKQAKDNVRRFAALLKDVPFFNDAEKTQIISQLREINPTVFT
                     EQSGDMETPLENETLAARLEKSGATVASLSAIEAMKARLDQLINVDIPKNSEEIGVAQ
                     EKGDLRENAEYKAALENQSILQAEAKQLEADIKAVQQLSLDDIDTKSVAVGTRVKLRD
                     VATGDIFIYSILDQWDADVDKGIISYKSPLGQALMGAHKGAQTGFNNQKLEIIGIEKA
                     IDASGRLI"
     gene            complement(5493..5915)
                     /locus_tag="PLY93_04935"
     CDS             complement(5493..5915)
                     /locus_tag="PLY93_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68856.1"
                     /translation="MKYFLGIFSILFITTLVAQADMEQRPSDSLSPDMIEAKPEGGFS
                     KDISGKWNCGDYGTMILADKRGKVAGSYTYNKGRVTGAVKNGIFKGTWKEGKGNNHGS
                     FEFQISTERMTPKPTNLRGKWKNQGESDWQESPWECAR"
BASE COUNT         1521 a         1322 c         1510 g         1863 t
ORIGIN      
        1 agcgagccag tcggaaatca tcggcgcgat gaatttatgc gattcgtggt agttggtata
       61 aaacgtggtg agtgagtgca ggttggccac catataaaaa cactcggctt cgttttgcag
      121 tttagcaaaa ttcgtgagca cgcctaaata atgaccaatg tgtaactcgc cggttgcttg
      181 catgccgctt aaaatacgac cgatgggtcg ggtgtcgtct gtgtttttgt gagttatgtg
      241 agtttcagcc attgcagcaa aaatccccct gtgataaaat tggtgcgcca aaaatcaaag
      301 cttaaaataa caagtacgat gaatgtaaga gctgtcacca acaccgcaaa aaatctccat
      361 tgcgtgtacg cagaaaaata tccggcggca gcgtcgaagg gaggtagtgg gataaaattg
      421 aagaggaacg ttgcccaggc ttgcgaaata aaaacagcgc tgaagcgcgc tgcgaacgta
      481 tcgggcgcgg gccccttaat ggccgcgtaa agaattccca tcaaaattaa aaataccaac
      541 cacagctggc cgtaaagaaa acgcaaaagc ggcaatggtt tattctggat gagtccaccc
      601 ggcgctgcgc cggtgagggt gaggcatgca aaagaggcga ttgaaaaatc ggctaacggc
      661 tgcgcatctt tttcgcctgc cattttcgac gcaaaccaag tgcggctaat gagtataatg
      721 agcgcgctgg ggattgtgat gaaaagcaaa cctgtttggt cgaataaaat gtgaaaaaaa
      781 ttcatccgcc ccctacgtag tgtatcaatt ccactgtgtc gttgtcgttg agtgtcgttg
      841 tcgtgtacgc gtttcggggt accagtgcgc cgttgagttc aacggcgacg cgctgcggcg
      901 caagcgaaaa atgcgtaatg agcgatgcta gagtgtgcgg tttggctacc gaagcgaggc
      961 ttaaactttc tccgttgaca atcatcgtgc tttcaaatac cctaaggcaa taattgcggc
     1021 cgactcgctg cgcaaaacat tttcagaaag taatactccg gttgcttttg tgcgcaaaaa
     1081 tataatttcc gatgggctaa atcccccttc ggggccgttg atgaacaagg tggatgttct
     1141 cttctcttgt tggattgttg tgaaaaattg tgttgccgat aaatctccaa aaaaagcggc
     1201 gtcttgtgcg gggaaaataa tggcatgtaa atctttgagt atatgcactg tgggtttgag
     1261 tggattatac gactgcatgc acgcgttttc taaaatggcg tcgcatcttt tttcgttgta
     1321 ttgttcgcgt tgcgaatggt cactcggtgt aaaataaata cgtcgtacgc ccaactcggc
     1381 tgccttctgc aaaataaccg agagcgtttt gggtttcacc catgcctgca ctaaatcgac
     1441 agtgtaaggg ggggataata taggaataat gggtgtgtcg gtggcgtgta ggtagttatc
     1501 cttgacttgg gcatggacga gtgcgcatcc gccgtcgctc acgagtgcag accacggtgt
     1561 gctgcggcgt aaaatacggt ggaaatgttc aacgtggcgc atgggtagct tcagcatttt
     1621 tttttcagaa aaaaaagaaa attcttcgtg gcttaataaa ataagatggt gttttttttg
     1681 taacgtgcgc aggggtgttg cgtgggcttg cacagcgttc tatttctcaa gattttcttt
     1741 tttgaaaagt gagtcgtcga gacgcgccgt tttgtcgata gaaaaaaatt ccagagtgct
     1801 caccgtgcct cggctcatgt cggccatttc ccaacgaaca ggatactcaa cttgctttgt
     1861 cgaggttttc gtttctttaa ttggtaatga gccgtactta aacgtcattg ttttcatgag
     1921 cgtcatcgcc gaatcgtagt aatcaatccg ttctaaacgg ttttgctttt tgggatcgac
     1981 taaaacaacg agtttaccgt aattaccttt ttccaaaggc aagttttcga taacggtgaa
     2041 ttccccgttt gcattttttt caacgccatt aattttttgt tgatagttat ccacgaaaga
     2101 gccattgccg atgtccagat acgcaaaacc ggaccctaaa atattttcaa agcggtctaa
     2161 atatttttta tggaaaagac gtttgtcgtg tgtcatgtac gcgtagatgt tttgtccttg
     2221 gttattgtag aggagcttga gcaccgtacc acgtgtcatt gaattgaata cgaagaaact
     2281 cgcatggtgt ttttggtata aactcacttt gtacacaacc gcacgcccgt ctttggtggt
     2341 gatggtcaac tgcaaacttt gatatccttc ggcgtattgt agttgttgaa agactccgtc
     2401 cataatctcg aacgcagtgc cgcccacccc tgcttttcct tgtgcgtgga ggagcggggg
     2461 gtgaagaacg acgataaagg cgagtataaa gagtaggcgt attttatgga ttatctttgt
     2521 aagtaaaaaa tacgcgctgt ttaaattttg tattctttct cgtaaacgcc tcatggctaa
     2581 gcttaaatca accgacctga tgcgtcaatg gctttttcaa tgccaatgat ctcgagcttt
     2641 tggttgttaa agccggtttg agcgcctttg tgagcgccca tgagagcttg gccgagaggc
     2701 gacttgtatg aaataatccc tttgtcgaca tcggcatccc actggtcgag gattgagtaa
     2761 ataaaaatat cgcccgtggc aacgtcgcgc agtttgacgc gtgtaccgac tgccaccgat
     2821 tttgtgtcga tgtcatcgag gctgagttgc tgcactgctt tgatgtcggc ttcaagctgc
     2881 ttggcttctg cttggagaat actttggttc tccaacgccg ctttgtactc ggcgttttcg
     2941 cgtaagtcgc ctttttcttg cgccacgccg atctcttctg agtttttagg aatgtcaacg
     3001 ttgataagtt gatcgagacg ggctttcatc gcttcaatcg ctgaaaggct tgcaaccgtt
     3061 gctcctgatt tttcgagacg cgctgccagc gtttcgttct ccaacggggt ttccatgtcg
     3121 ccgctttgct cggtaaaaac tgtgggatta atttctctca gttgagagat aatttgtgtc
     3181 ttttcggcgt cgttgaagaa aggcacgtct ttaaggagcg cagcaaaacg gcgcacgttg
     3241 tccttcgctt gtttttcaat cgtgtcgaaa atttcttgtt tggcgttgcc aaagaaaata
     3301 tcgcgtgcgg tgtttttgag cttcgagccc ttttgttcga ttttggggag cgatttgagg
     3361 atacgaaaca atcccaaaag gctttcttgc gtttgttcat tcgagagttc aaagctaccg
     3421 gcgacaattg atttaaccat ccacaaaaat acttcggcat tgtctttcgc ttctttgcgg
     3481 atgcggttga cgaagtcggt gatttcggtg ttcgcctctg ctgccataag ttctgaaaga
     3541 attgtcttat gcagcttaat cggtgtttca aataaaagtt cgctataaat tttccgccat
     3601 tctgcatggt ggcgttttgt gaacttgagg ccatgccgtt tcagttcgga ttgtttaatt
     3661 tcgcttaaaa attcagtcag ctctgctaca ttcatcgctt gaaaagcggc gataatttcc
     3721 gcttgttggt ctttcgcaaa aagcggttcg tcgtcggcga gcgcttctgc cgctaactcg
     3781 aggagccaaa gacttttcaa gcgggtgttg aggtcaagac tcttgagagt ttctttgaag
     3841 tgtggagcga taaattcaat cgcttcaaaa ttctctgtgg gcaatttgag cgcatcgaca
     3901 aatgccaata cgcggtcttc aaaaccgtgt gccgcttgga atttttccat caccacctcg
     3961 gcttttgaaa ccggcgtatc gtgcagttca atggtgtctt tgcgctgtgg tgaaatatcg
     4021 atgaggtcgt ctttcatgag ttcggggcgt gtttttcccc accacttcga ccattgcgtc
     4081 aacggtacat atttgtcggt gagttcggtt ttaatatcgg aaagcgaaag tttaccgccg
     4141 aacgagtgta atagaatttt gaaaaattgg gtaatgtctt ctttgaaaag ctttttcact
     4201 tcatcgggct tttcaaactg cattacccaa aagtgctctt cgcgtaacgg tttgagcgac
     4261 ttgagcgcca tttggatgtc catcttgtgc gcttttttat ctttgaaatc gataaccatc
     4321 tcggcagtcg ttaaatcgat gattttaccc acgccccaac tgcggtggaa gacatagtta
     4381 cccttgtcga aaacgatgtt cgtttcaaaa ttgaggattg acgagctgag gcttttacgc
     4441 gagtctaaaa ggccagagta gcgaatgaaa tcttcaagca acgagtgcga tttatacttt
     4501 tcgcgatacg tacgcagtaa ttcatttttg aatcgtacat agttgtggtt gtattctaaa
     4561 atctttttac ttaagaggat tactttatcg tggtcttctt ttttaataaa gtaattcgcg
     4621 agcaatacgt aaagctcggc gatggtttct ttttgacggt gaccggcgag gatacgttca
     4681 atttttcggt agaaagtaaa atcgtcgggc aataactcga tgaggcgatt ccatgtcgtt
     4741 ttgagttttt caaattgtaa gttgcgcgcg aacgcttcgg cggcttgctt gtaaaattga
     4801 atgcctttct ctaaatcttc ggcgataata gcatcggcgt acttaagggc gatgtcggga
     4861 tttttgcggt cgagtaaagc gagttgctct aaaacgggga tagcttcttt atgtttgcct
     4921 aatttttcga gtgcattcgc acgcgcgcgt aaaatggtac gcgttctggt gaccgcgagc
     4981 attttctcag agaggtaatc gactaccgcc cacttcgacg cctcttggaa ctggtcgagc
     5041 aatgttttta agtgaataat ttcatcgggg atattttttt cgagcgccat catgcccaag
     5101 aaataatgcg cggcgatcga ttggtcgtac tcggttaagt gcgtgagaga ttcggagcgt
     5161 aatacgccgc tcttattttg ttttttaact tcgtcaaaaa tttgctctaa aattttgaac
     5221 cgtgaaatgc tgacgtcttt cgccgaaagg cgtgtccatt tttcttcgtt aaactggctt
     5281 tgaatgcgcg cttcaagtgt gtcgctttcg cctgcggtcg ccgcttcttt tgttgtttct
     5341 acttcgctca tggaaaatct ccttcggtgg acgtgtccaa aaaaaaagca gtagcaacag
     5401 ccactgcttt tgtacaaaga aagaaggaaa agtcagcttg tcaagaactt gtgaacctct
     5461 acagcagatt taaactgcag taaaacgtga gattaccgcg cacactccca aggactttct
     5521 tgccaatcgg attcgccttg gtttttccat ttcccgcgta ggtttgttgg tttgggtgtc
     5581 atgcgttcgg ttgaaatctg aaattcaaaa ctaccgtggt tgtttccttt gccttccttc
     5641 cacgtgccct tgaaaatgcc gtttttcacg gcgccggtta cgcgaccttt gttgtatgta
     5701 taactcccag cgactttacc gcgcttgtcg gctaatatca tggtgccata gtcgccacaa
     5761 ttccattttc cagaaatgtc ctttgaaaaa ccgccttcgg gcttagcctc aatcatatcg
     5821 ggggacaaac tatcgctcgg ccgctgttcc atgtccgctt gcgctacgag agttgtgatg
     5881 aacaatatag aaaaaattcc taagaaatac ttcatgggag aactcctttt aactacagta
     5941 ttttagggat gacccccagc agagcatagt atcgatatac tcttagacgt aaagtctgtt
     6001 ttgaaagatt gccttcctat gaaagcttat tggaatatgc cgaacgaacg tatcagataa
     6061 aatgcgtgta ccgtttcaat aaggattttt cctagcagcc tgtcgggctt ttcccttgcg
     6121 gcatgacata aactctgtgg acgacctctc taagcaacac ggttatgcat aatgagacgc
     6181 tttcagaacg cagacagaga gacggcattt ctgctg
//
LOCUS       DAOMPD010000104         6197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5399_length_6197_cov_2.598828, whole genome shotgun sequence.
ACCESSION   DAOMPD010000104 DAOMPD010000000
VERSION     DAOMPD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5399_length_6197_cov_2.598828"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLY93_04940"
     CDS             complement(<1..252)
                     /locus_tag="PLY93_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68857.1"
                     /translation="MKKAIPQNRDLACFTRNACKTGGKILFCGILSFFSTFGLSAQPA
                     AVDTPMDFPAPGEGATIYKMPAADGALAKIRKKYFYLGEL"
     gene            complement(249..1436)
                     /locus_tag="PLY93_04945"
     CDS             complement(249..1436)
                     /locus_tag="PLY93_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68858.1"
                     /translation="MTIINKQVFTKCRRLLAAFVAVSVILCSVGTAHLYAQGKGVQLK
                     KKTPKKRIDAKGNLVEDIAAVEDMYDEGNIGSIAQLDPRAGRAFFAHTWFVKASATIN
                     YFNLGSDFQNVIKQIEGAFGGDTLNMKILAFNSDISMATGEKVARAPTHFLPTGNMSL
                     GFYFGRHQLELEFGLAGLVPLKTVDLDTTMTLTDKTVVGSSAQCTTAANCPLANLGFV
                     NGTSRQGQYKFQMTMNEDVWILTPSLSYDYVFLVRKWGRMSGGLGVGAMILSTSQQIA
                     FKAVRTDLSSVEAPLDYLKSRVLQGTANSTNVTDIGPIFRLHFTYRPPPITKFLNTQL
                     EIRAGFNYGFVYLNRDVDGTGQAILGDTLVGSFPLSSLGFKTQEVNKFELMGGFIQVG
                     IVF"
     gene            complement(1433..>2345)
                     /locus_tag="PLY93_04950"
     CDS             complement(1433..>2345)
                     /locus_tag="PLY93_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68859.1"
                     /translation="ATLPTTDIVNPPLKVHGKEYTGAVPFFTIALGSSLVLPNANTAT
                     EAQNTSLLYMLRQNEAIYTAPDAVLPPAEAGLAAMPALRIEGELPFTRLSLLPQWQKA
                     AALFSLEVGFSTQQNLFSTTGNFRFLNNQAGALELTDVTYSGKIIASERKQYFSPMLG
                     LSFEWWSYANVHFLTRAAVGFFLQNGIRTYHFNTDNQNITSVSNPTYSGSYHLEAHFT
                     ETYSYAFLAAGRLELGVRYEVWPKIHVALLVSSTVLYGMLPLDIVGGFTEYGNGKKTY
                     EYVVSSIRDEASLVVLPSVFLALSYEL"
     assembly_gap    2346..2355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2356..2624)
                     /locus_tag="PLY93_04955"
     CDS             complement(<2356..2624)
                     /locus_tag="PLY93_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68860.1"
                     /translation="MRFVRIVIPLVMVSSFFAVQKDNPKVKKKPAVRVETPTRAEKSL
                     PSVQDQGAEGRTPPPTESPVGVVNEVHDGASSPPLDNSSFGLVNET"
     gene            complement(2621..3616)
                     /locus_tag="PLY93_04960"
     CDS             complement(2621..3616)
                     /locus_tag="PLY93_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68861.1"
                     /translation="MKKYNTQNADTRKVKACTRSIFVFLFIGFTLFPVSALRLSFGMS
                     GEIATLQNSMLKEQLISLLGQKQSIPLVGVSSGNAYQSAWDAEGSLSEAYLIRPIPEL
                     RMAVDFKTYSDFTFSFFAALSGFLPLTTTYNTGVYRVRASNTCGSVDYATCPPAAMNF
                     VDSDGVGEYAGEMRSNLRVAQAAVGVGVSQKIGAYWQGMWYWGMDLGVAAQTFSIAST
                     FIASRCSTGGVTPCAQGNQVRVVQSELKTMALYTLGPLWGANIRYERENSWWFAEAGV
                     TILFLFSHWENSGYTNFVANDTQVFTYNAQDMGVPAAQNNFSALPSFILRFGVRL"
     gene            complement(3674..>6197)
                     /locus_tag="PLY93_04965"
     CDS             complement(3674..>6197)
                     /locus_tag="PLY93_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68862.1"
                     /translation="KVGDLLQGVTNTDKIIDLIWGVTITSNLSTVINGIFKSSEIPGC
                     SDGTAKTYVGPSTINASDTGTCEQSGNGFFYGERDQAVRTLVHLVENVAVAQKLIDMI
                     DGVAPAKVIDLVNHTSTYCQQPDATAHVYPRAVTPAQPYAGQRLVKIVDGTTTISQLV
                     YVTNNVSSIIKMSKLVSFMDIGVGPNDGTGKMATIINTIAGTNCYLSTTVPGRGNVTA
                     VAPGGSAACVAQNSQNATGMGKMVNVIEFVDGATVSDAMMQVRNLLNDVTDVGKTADL
                     INQTINSSNIVGLLNGVIDARYGTSTTDLVTLLNDLPRGEVSKMISLVGEIGNATGTA
                     TRTFPGNDHVLIAQLMSSYNAAALTSTSGVGVTNMSNLIGVLKTTGGQNYTTAPTVGG
                     LNAGGGSGATATAIVPSTLSPTPAPGAITKITLTTEGVCTVAPTAVTAVAGGGVGATF
                     GVVTAPIGGAATFKVTAVYVKNGGSGYSANVNALTFNGTCGTAPVAASTVNGVIGVSI
                     TNPGTNYTDSFNTVTFTGGGGTLASGTALVAGTINTLANFSGGTGYTTGDVCPIIGAG
                     GAGGTCTVTATAGALTGCSAISGGSDYIDGQVVTIGGAAVVRADVTAGAVSGYTIISG
                     GCGYSGAPSVVVASGALDCTVPPAAATATLTSGRVTAIAGGIGGSGCKENPRVSVGES
                     PYATHGDGATAIVNNVSTGKVVAISLSSAADNLAQLLANTEKAPGSTVINYNSIAPNI
                     STREAMVRLLHHGADHASAGYVSSPFLYSGVGGVHIGQSVLNNLTGPISTMTIINMLN
                     SNSTSMTDLVVMIGCGDHVMYTPPAGRQSFYTGCSSHTPSLW"
BASE COUNT         1616 a         1619 c         1411 g         1541 t
ORIGIN      
        1 aagctcgccc aagtaaaaat attttttgcg tatcttcgcc agcgctccat cggcggcggg
       61 catcttataa atcgtcgcgc cttctccagg agccgggaaa tccatcggcg tatcgacggc
      121 ggcaggttgt gcggaaagac caaaagtaga aaaaaaggat aaaatcccac agaataaaat
      181 ttttccgcct gttttacatg cgttgcgtgt aaaacaggct aaatctctgt tctgtggaat
      241 tgcttttttc aaaacactat ccccacttgg ataaatccgc ccatgagttc aaacttattg
      301 acttcttgcg ttttgaatcc taacgacgac aacgggaaag aacccaccaa cgtatcgccc
      361 agtatcgctt ggccagtgcc gtcgacatcg cggtttaagt acacaaaacc gtagttaaaa
      421 ccggcgcgga tctcaagttg agtgttcaaa aattttgtga tgggtggtgg acggtacgta
      481 aagtgcaagc gaaaaatggg accaatgtcg gtcacgtttg tagaattcgc cgtcccctgc
      541 aacacgcgtg attttaagta atcgagaggc gcctccaccg atgacaagtc ggtgcgtact
      601 gctttgaacg caatttgttg cgaggtggag agaatcatag cccccacacc caagcccccg
      661 ctcatacgtc cccatttacg cacgaggaaa acatagtcat acgaaagcga cggggtgaga
      721 atccatacat cttcgttcat cgtcatttga aatttatact gcccttgtcg cgacgtaccg
      781 ttcacaaaac ctaaatttgc caacggacaa ttcgccgctg ttgtgcactg cgcgctcgaa
      841 ccgacgactg ttttgtcagt aagcgtcatg gtggtatcta agtcgacagt ttttaatggc
      901 acgagccctg caagaccaaa ttcgagctct aactgatggc gaccaaaata aaaccccaac
      961 gacatattac cagtgggcaa aaaatgggtg ggtgcacgcg cgaccttttc tcccgtcgcc
     1021 atggaaatgt ccgaattaaa cgcgagaatt ttcatgttca gagtgtcgcc accgaacgcg
     1081 ccttcgattt gtttaataac gttttggaaa tcagaaccta aattaaaata gttaatcgtc
     1141 gccgacgctt tcacaaacca agtgtgtgca aaaaatgcac gccccgctcg tgggtctaac
     1201 tgcgcgatag agccaatgtt accctcgtcg tacatgtctt cgacagcggc aatgtcttcg
     1261 actaaattac cttttgcatc gatacgtttt ttaggcgtct tttttttaag ctgcacgcct
     1321 ttaccttggg cgtaaagatg cgctgtgccc acagagcaca aaattaccga cacggcaaca
     1381 aatgccgcta aaagccttcg acatttagta aacacctgtt tatttattat tgtcataatt
     1441 catacgagag tgccaaaaaa acacttggca gtacaaccaa acttgcctcg tcgcggatcg
     1501 acgagacgac gtattcatac gtctttttac cgttgccgta ctcagtgaaa cctcccacaa
     1561 tatccaacgg cagcatacca tataagacgg tgctcgagac taaaagcgcg acatgaattt
     1621 taggccatac ctcgtagcgt acgcccaact caagacgccc tgccgctaaa aaggcataac
     1681 tatacgtttc tgtaaaatgt gcttcaaggt ggtagctccc cgaatacgta ggattactca
     1741 cagaggtgat gttttgattg tccgtattaa aatgatacgt gcgtatacca ttttgtaaaa
     1801 aaaatcccac tgcagcccgc gtcaaaaaat gcacgttagc ataactccac cactcaaaac
     1861 tcaaacccaa cattggcgaa aaatactgtt tgcgttcgct ggcaataatt ttacccgagt
     1921 acgtcacatc ggtgagttca agggcgccgg cttggttatt taaaaaacga aaattgccgg
     1981 ttgtcgaaaa taaattctgt tgcgtgctaa atccgacttc taaggaaaat aatgcggcgg
     2041 ccttttgcca ctgcggcaaa agcgaaaggc gggtgaacgg tagctcgcct tcgatgcgca
     2101 gtgcaggcat cgccgccaaa cccgcttctg caggtggtag taccgcatcg ggggcagtgt
     2161 agatggcttc gttctgacgc aacatataaa gaagactggt gttttgcgcc tcggtggccg
     2221 tgttggcatt gggcaatact aaactcgaac caagagcgat ggtgaaaaaa ggcaccgccc
     2281 ctgtgtactc ttttccgtgg actttgagag gaggattaac tatgtcagtc gttgggagtg
     2341 tggcgnnnnn nnnnngtttc attcactaat ccaaaggaag agttatccaa tggcggacta
     2401 ctggctccat cgtgcacttc attaactacc cccacaggcg actcagtggg cggtggagtt
     2461 ctcccttctg cgccctgatc ttgaaccgaa gggagtgatt tttcagctct tgtaggagtt
     2521 tccacccgaa ctgctggttt ttttttaact ttagggttgt ctttttgcac cgcaaaaaaa
     2581 cttgaaacca tgacaagagg gataactatc ctcacaaatc tcatagccgt accccaaaac
     2641 gcaaaataaa cgacggtagt gcagaaaaat tattctgtgc cgcaggaact cccatatctt
     2701 gagcgttata cgtgaatacc tgtgtatcat ttgctacaaa attggtataa ccgctatttt
     2761 cccagtgcga aaaaagaaag aggatagtta cccccgcttc ggcaaaccac cacgaattct
     2821 cgcgttcgta gcggatattt gccccccaaa gtggccccaa cgtgtagaga gccatcgtct
     2881 tgagctctga ctgcaccacc cgcacttggt taccctgcgc acacggcgtt acgccccccg
     2941 tcgaacagcg cgacgcaata aacgtcgatg caatcgaaaa cgtctgcgcg gcgacgccta
     3001 aatccatccc ccagtaccac atcccttgcc agtaagcgcc tattttttgc gaaacaccca
     3061 cccccactgc tgcctgcgct acgcgcaggt ttgagcgcat ttccccagcg tattccccca
     3121 cgccgtcgct atccacaaag ttcatcgccg ccggcgggca agttgcgtaa tcgacgctgc
     3181 cgcaagtatt gctcgcgcgc acgcggtaca caccagtgtt gtacgtcgtg gtgagcggta
     3241 aaaatccaga gagcgccgca aaaaaactaa acgtgaaatc ggaatacgtc ttgaaatcta
     3301 cagccatgcg taactcgggt atgggcctga tgaggtatgc ttcgctcaaa gaaccttcgg
     3361 catcccacgc gctttggtac gcgttccccg acgacacgcc cacgagcggg atgctttgct
     3421 tttgtcccaa aagagagatt aactgctctt tgagcatcga gttttgtagt gttgcaattt
     3481 cgcccgacat cccaaaagat aaacgcagtg cactaacggg gaaaagagta aatccgatga
     3541 ataaaaaaac aaaaatgctg cgtgtgcatg ccttcacttt tcgagtatcg gcattttgcg
     3601 tattgtactt tttcattgaa actctaaaaa aaaggccgct tacgcggcct tttacgtcaa
     3661 acgatactca tgactaccac agcgacggcg tatgcgaaga acaaccggta tagaacgatt
     3721 gccgtcctgc tggcggtgta tacataacgt ggtcaccgca accaatcatc accaccaggt
     3781 cggtcatact ggttgaattt gagttcaaca tattgatgat tgtcatcgtt gagattgggc
     3841 ctgtcaaatt attgagaaca ctttgaccaa tatgtactcc ccccactcct gaatagagga
     3901 aggggcttga tacataacca gcgcttgcat gatctgctcc gtggtgtagt aagcgtacca
     3961 tcgcttctcg tgtagagatg ttgggagcaa tcgagttata atttatgaca gtgctaccag
     4021 gcgctttctc agtgttcgct aagagctgcg ccaagttgtc cgcagcagat gaaagactaa
     4081 ttgccaccac tttacccgtt gaaacattat tcacaatagc cgtcgcacca tcaccatgcg
     4141 tggcgtaggg agattcaccc acactcaccc gaggattctc tttacagcca gaaccgccta
     4201 taccaccggc aatcgctgtt acacgcccac tggttaaagt agctgttgcg gcagcaggcg
     4261 ggacggtgca atctaaagcg cctgaagcca caacaactga cggtgcgccg ctataaccac
     4321 agccccctga aatgattgta tagccagaaa cagcgccagc agtcacgtcg gcgcgcacca
     4381 ctgctgcgcc gccaatcgtc acaacttgac catcgatata atccgagcct cctgaaatgg
     4441 cactacaccc tgtgagcgct cctgcggttg cagtcacagt acatgtgccc cctgcccccc
     4501 ctgcgccaat aatgggacat acatcgccgg ttgtataacc ggtgccaccg ctaaagttgg
     4561 ccaacgtgtt gattgtacca gcgaccaatg ccgttccaga cgcgagggtt ccgccaccac
     4621 cggtaaaggt aactgtatta aaactatctg tataatttgt acccggattg gtaatagaga
     4681 ctcctattac gccattcacc gtcgatgccg ccaccggtgc tgtaccgcat gtgccgttaa
     4741 aagtgagagc gtttacattc gcagaatatc ctgaaccgcc atttttcacg tagacggcag
     4801 ttaccttgaa tgttgccgct cctccaatag gcgctgtgac aacaccaaac gttgccccca
     4861 caccgccacc ggcaacggct gtgacggctg tgggcgccac agtacagaca ccctctgttg
     4921 ttagagttat ttttgtaatc gcacccggag ccggtgtggg cgacaaggtt gatggaacaa
     4981 tcgctgtggc cgttgcgccg cttccaccac cagcgttgag tccgccaacc gtgggtgcag
     5041 tagtataatt ttggcctccg gttgttttaa gcacaccaat aaggttactc atgtttgtaa
     5101 cacccacccc cgaagtgctc gttaatgcag cagcattata agaagacatg agctgtgcaa
     5161 tgagaacatg atcgtttccg gggaatgtgc gtgttgctgt gcccgtcgca ttgccgattt
     5221 caccgacaag agaaatcatt ttagagactt ctccgcgtgg caagtcgttg agtagggtca
     5281 caagatctgt cgttgaagtg ccataccgcg cgtcgataac cccattcaaa agccccacga
     5341 tattcgatga attgatagtt tgattaatga ggtcagccgt tttacccaca tcggtgacat
     5401 cgttgagtaa attacgcact tgcatcatcg catcggaaac agttgcccca tcgacgaact
     5461 cgatgacgtt caccatttta cccatacccg ttgcattttg actgttttgt gcaacgcaag
     5521 ctgcgctacc gccaggcgct acggccgtca cgttaccgcg accgggaact gttgtactta
     5581 aataacagtt tgtacctgca atggtattga taattgtcgc cattttgcct gtaccgtcat
     5641 taggaccaac cccaatgtcc atgaaagaaa ccaattttga cattttaata atcgaactga
     5701 cgttattggt gacatacaca agctgactaa ttgtggtcgt accatcgaca attttaacca
     5761 accgttggcc tgcatacggt tgagcgggag taaccgcacg agggtataca tgcgctgttg
     5821 cgtctggttg ttggcaatat gtggatgtat ggttgactaa atcaatcact tttgccggtg
     5881 caacaccgtc gatcatgtca atcaactttt gtgccaccgc cacattttct actaagtgca
     5941 ccaacgttcg taccgcttga tcgcgctcgc catagaaaaa tccattccct gattgctcgc
     6001 atgtacccgt gtcggatgcg tttattgtgc tcggtcctac gtatgttttt gctgtaccat
     6061 cgctacaacc cggtatttca gatgatttaa aaataccgtt gattaccgtt gaaagattac
     6121 tcgtgatggt cactccccat ataagatcga tgattttatc ggtgttggtg actccttgga
     6181 gtaaatcccc caccttt
//
LOCUS       DAOMPD010000105         6173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5425_length_6173_cov_2.917620, whole genome shotgun sequence.
ACCESSION   DAOMPD010000105 DAOMPD010000000
VERSION     DAOMPD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_6173_cov_2.917620"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLY93_04970"
     CDS             <1..353
                     /locus_tag="PLY93_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68863.1"
                     /translation="KRTQIDPKSVSYLCGLHNKVMLYISQNEHTRKLRIVNVYSGRRK
                     YSEQLLRDIEVLDREYPQIHIEFIARRGEFGPEFIQKLSDEWKIPVNFMFIGSLGDRF
                     PYRVEELGGVRLII"
     gene            complement(425..1063)
                     /locus_tag="PLY93_04975"
     CDS             complement(425..1063)
                     /locus_tag="PLY93_04975"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRP68864.1"
                     /translation="MRTLTKDMQQALTPAEALDVLKKGNERFVQNLRANRNLLAQVNQ
                     TSDGQYPFAVILSCMDSRTSVELIFDQGLGDVFSIRIAGNVINEDILGSIEFAVKLAG
                     CKIVVVLGHTKCGAVTGACNHVEMGNLTGLLKKISPAIQAEHTIVNNRNGTNESFVER
                     VIDLNVANSIREIRRNSPIIRDMEQQKIIEIVGGVYDIDSGTVKFFASDALG"
     gene            complement(1195..2934)
                     /locus_tag="PLY93_04980"
     CDS             complement(1195..2934)
                     /locus_tag="PLY93_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP68865.1"
                     /translation="MYRFLISLLFIHGSALSLFAQAPLKASAQPSPPVITTPPTTSQS
                     TKPAVPTVPGVMAPAKSDTVVTSVEPKGAPVVVGGKEYFRIFSDDGPYTAAERAQIIG
                     EKLSKILNNENPDPALLRAERDKDESRLFYDKTQLCVITANDAAIQKKSRYAIEQEYL
                     KMLGEGIKTRKQSFDLNSLIRGAGFSLIATLGLALLIKLLFYGIRKLSGLIDSWRNTR
                     IGTLKIQNVELLSADRITDIVLALLRFVRFVILLTLLYFYLPFLFSFFPWTQGWADIL
                     FGYILSPLKAVGAAIIGFVPNLFFIGVIIWVTRYLIRVVNMIFTEMGRGTVNFSGFHP
                     EWAAPTYKIIRFLIIAFSGVMIFPYLPGSNSPAFQGVSIFFGVLFSLGSTSAIANVVA
                     GVVLTYMRPFKEGDLVKIADTVGDVMEKTLLVTRIKTSKNVVVTVPNSMILSTHMTNY
                     SSSAKDPGLILHTSVTNGYDVDWRVVQSLLLSAAKTVEEIEQDTPPFVLQTSLDDFYV
                     NYELNAYTERPNQMAVIYSKLHAAILDTFNAAGIEIMSPHFYGLRDGNAVNLPADSLP
                     KKYQAPAFRIKGT"
     gene            3519..3803
                     /locus_tag="PLY93_04985"
     CDS             3519..3803
                     /locus_tag="PLY93_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68866.1"
                     /translation="MESRIDTVKDKALPVAVAQRNRPVAVDPQNDTRNLKADAGTVVA
                     DAQLAPSAATEMSKPQGEANQTLILVSLGAALAAIMGIAYFVRKTKATQH"
     gene            3832..4203
                     /locus_tag="PLY93_04990"
     CDS             3832..4203
                     /locus_tag="PLY93_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRP68867.1"
                     /translation="MARLNKLEFLRFGNYSLTAGGLLEFGAFILFIWLVLRAVRLLVF
                     RSRYLDTSRRYAAFSLIRYITLTITTLLGLRILGVNVSVLLAGSAALLVGVGLGLQSL
                     FSDFVSGIILLLDRTIKVGDI"
     gene            4401..5945
                     /locus_tag="PLY93_04995"
     CDS             4401..5945
                     /locus_tag="PLY93_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HRP68868.1"
                     /translation="MKKIKNIKLEYLKMQDYEDLKDAMVKAYITMPNAYWKESQISSL
                     ISRFPEGQVVVKADGRIAGCALSIIVDYDSFDNRHTYREITADFTFKTHSAQGDVLYG
                     IDVFVHPDFRGLRLGRRLYDYRKELCERLNLKAIVFGGRIPNYHKYASELSPKEYIQK
                     VRERKIHDPVLNFQMSNDFYPSKVLRGYLEGDQASGEYAVLLRWDNIYYEKPSTKPAA
                     KKTVVRLGLIQWQMRPYKNLDDLMRQAEYFVDALAAYRADFALFPEFFNVPLMAEQEK
                     MNEPDAIRALAEYTPAIARRFQELAIRFNINIITGSMPDISDGKLINRGFLCRREGST
                     DSYEKLHVTPDEAKVWGMVGGNKLTAFDTDCGKIGILICYDSEFPELARMLADEGMEI
                     LFVPFLTDTQNAYSRVRLCTQARAVENECYVAIAGNVGNLPNVHNMDIQYAQSAVFTP
                     CDFAFPSNGIKAEATANTEMVLVADVDLSLLRELHQLGSVRNLRDRRHDLFELKRRDQ
                     IPVNTQ"
BASE COUNT         1598 a         1504 c         1489 g         1582 t
ORIGIN      
        1 taaaacgcac tcaaattgac cccaaaagtg tcagttatct ctgtgggttg cataacaaag
       61 tcatgcttta tatttcgcaa aacgaacaca cgcgaaaatt acggattgtc aacgtttata
      121 gcggcaggcg gaaatattct gaacaattat tgcgagatat cgaagtgctc gaccgcgaat
      181 atccccaaat ccacatcgag tttatcgccc ggcgcggcga gttcggtccc gagtttattc
      241 agaagctctc cgatgaatgg aaaatcccag taaattttat gttcatcggt tcgctgggcg
      301 accggtttcc ttatcgcgtg gaagaactgg gcggtgtgcg tttgatcatc tgaacggcaa
      361 ccgcaccgaa attagttcgg cagccactgg gatgagaatt gaaaagaagt gaaataattg
      421 tacgctatcc taacgcgtcc gatgcgaaaa atttcacagt gccggaatct atgtcatata
      481 ctccgcccac tatttcgatt atcttctgct gctccatatc gcgaatgata gggctattcc
      541 ggcggatttc gcgaatcgaa tttgcgacat ttaaatcgat cacgcgttca acaaaactct
      601 cgttcgtacc gtttctatta ttcacgatgg tatgttcggc ttggatagct ggactaattt
      661 tcttaagcag ccccgtgaga ttgcccattt cgacatgatt acatgctccg gtgaccgccc
      721 cgcacttggt atgtcctaaa acaacgacaa tcttacaacc tgcaagcttg acggcaaatt
      781 cgatgctacc caaaatatct tcgttgatca cattcccggc aatgcggata ctgaagacat
      841 cccccagccc ctgatcgaaa atcaattcga cagaagttct ggagtccata cagcttaaaa
      901 ttacggcaaa cggatactgc ccatcgctgg tctgattgac ctgagcaaga aggttcctat
      961 tggctctgag attctgaacg aatcgctcat tccctttttt taatacatcg agcgcctcag
     1021 ccggggtcaa tgcttgttgc atatctttag ttaaagttct cacaaaatct cctcatgtgc
     1081 taaatttatc ttaaattctc attcgcggct tctgttcagg gacagtattt tcagacctag
     1141 gcgctgcttc gattaccgtg gttatcacga ccaaaaagaa caaacactgt actttcacgt
     1201 tcccttgatc cgaaaggcag gcgcctgata ttttttcggc aatgaatcag ccggcaaatt
     1261 cactgcattt ccgtcgcgta agccataaaa atgtggcgac ataatttcta tacccgccgc
     1321 attaaaggtg tcgagaatcg cggcatggag tttggaataa atgaccgcca tctgattcgg
     1381 gcgctcggta taggcattga gttcgtaatt tacataaaaa tcgtcgaggc tggtctgcag
     1441 cacaaacggt ggagtatcct gctctatttc ttcgacagtt tttgccgccg agagcagaag
     1501 cgattgtacc acgcgccaat cgacatcata gccgttggtc accgaggtgt gtaaaatgag
     1561 tccaggatct tttgccgatg agctgtagtt ggtcatgtgt gtactcagaa tcatggaatt
     1621 tggaaccgtt acaaccacgt tcttgctggt ttttattctc gtgaccagga gcgtcttttc
     1681 catgacatcg cccaccgtat cggcaatttt gaccagatca ccttctttaa aagggcgcat
     1741 gtatgtgaga acgacgcctg ccaccacgtt ggcgatggcc gaggttgaac cgagcgagaa
     1801 gagcacgcca aaaaatatgg agactccctg aaacgcgggt gaattagaac ctggcagata
     1861 aggaaaaatc atcacgcctg agaaagcgat gatgagaaac cgaataattt tatacgtagg
     1921 cgccgcccat tcgggatgga atccggaaaa attaaccgtc ccgcgcccca tttcggtaaa
     1981 tatcatgttc acaacccgga ttagatagcg ggtaacccaa atgataaccc cgatgaagaa
     2041 gagatttggc acaaagccaa tgatagccgc ccccaccgct ttgagaggcg acaaaatata
     2101 accgaacaga atatctgccc agccttgcgt ccacggaaaa aaactaaata aaaacggtag
     2161 ataaaagtag agcagcgtta gtaaaatcac gaaccgcacg aaacgcaaga gagcgagaac
     2221 aatatcggta atcctgtctg ccgagagcaa ttcgacattt tgtattttta gcgtcccgat
     2281 acgcgtgttg cgccaactat cgataagccc agatagtttg cgtattccgt aaaacaacaa
     2341 cttgatcagc aaggcaagac cgagcgtggc gatgagagag aatccagcgc cccgaattag
     2401 gctattgaga tcaaaagatt gcttgcgtgt cttgatgcct tcgcccagca tcttgagata
     2461 ttcttgttca atggcatacc gagatttttt ctgtatcgcc gcatcgttcg ccgtgatgac
     2521 gcataattgc gttttatcgt aaaacaacct cgactcgtct ttatcgcgct ctgcccgtag
     2581 taaagccggg tccggatttt cattatttaa gatcttgctc aatttctcgc caatgatctg
     2641 cgcacgctct gccgcagtgt acggtccatc gtcgctaaat atgcggaagt actctttgcc
     2701 gccaacgacg actggcgcgc ctttaggctc gacggaagta acgacggtgt cgctctttgc
     2761 gggcgccatc accccaggca ccgtgggcac agcgggtttt gtgctttgac tcgtggtggg
     2821 gggagtcgtg atcacgggag gcgaaggttg tgccgacgct ttgagcggcg cctgggcgaa
     2881 caaagacaga gccgaaccgt ggataaacaa aagtgaaatc aggaaacggt acatccacaa
     2941 ttgtcattgt ttgcctgaca ccgtcaaaca gatttgactc ggtattttct cgtggatata
     3001 gaaaccatac aatcattttt acatatcttc gcggactggc atcatcggaa gattttttct
     3061 gctcattctt ttattcgtcc tcatctgggt ggtatgtcgg ttgcgataag cgttgtcggc
     3121 gccggtgtgg cgaccgcgta caattaggtg gcatcaaagg tgacgttgct gatattggcg
     3181 tcctacgcac acgttcatgg agctggggca gtgggtagat ggcgatctct acaacggtcg
     3241 gattgtccgc attgcgaata gctttgttgt tgccatggac tatttcaggc ttttttcctt
     3301 gacatttgcg ttttttgtgc tatgctcttt ttaatgtaaa gtaccaaagg aggtatagca
     3361 tgaaaaactt cattccattc atagaaaaga aaattacctt tgtggcgata gcgctgctgg
     3421 gctgcttatt cattcttgcc tgcgcgacta cctaaacgcc tacagcgtca gcaacgcaaa
     3481 actccgcgaa tcaagattta agccaaccac cggttttaat ggagtctcga attgatacag
     3541 tgaaggacaa ggcattgcct gttgcagtgg ctcaaagaaa tcgtcctgtt gcagtagatc
     3601 cccagaacga cacgcgaaac ctgaaagccg atgcgggcac cgttgtggcc gacgcgcaat
     3661 tggctccgag cgctgccacg gaaatgtcaa aaccccaggg tgaggctaat caaaccctga
     3721 tccttgtttc actcggcgct gccctagctg cgattatggg tattgcctac ttcgttcgca
     3781 aaaccaaggc aacgcagcac taacgtaggg cagagcccaa tattgaataa aatggcgcgc
     3841 ctgaataaat tagaattcct ccggttcggc aactacagcc tcacagccgg aggtttactg
     3901 gagttcggcg ccttcattct atttatctgg ttggtcttac gcgctgtgcg attgctggtt
     3961 ttccggtcac ggtatctgga taccagccga cgctacgccg cttttagcct tattcgttac
     4021 ataacgctga caatcacaac tttgctcggg cttcgaattc ttggtgttaa tgtctcggta
     4081 ttgcttgccg gttctgcagc actgcttgtg ggcgttggct tggggctgca aagcctgttt
     4141 tccgatttcg tctcgggtat tattctgctc ctcgatcgaa caatcaaggt gggcgatatc
     4201 tagagcgata tccggcaaaa tatttggcaa ctgtttcgcg aaaaccgtat tattattcct
     4261 ttcccacagc gtgttttgca atgggcaaat ccaagttcgc ccaaacggcg gcggcgcaaa
     4321 tagcttatgg attcaccgga aatccgcctt gataccgtgc cacccaaggg tcgcccaatt
     4381 ttttaacagg agaaaattaa atgaagaaaa tcaaaaatat aaaactggaa tatttaaaaa
     4441 tgcaggatta tgaagatctt aaagatgcca tggtcaaagc ctatattacc atgccaaacg
     4501 cctattggaa agagagccag atttcttctc ttataagtcg ctttcccgaa ggccaagtcg
     4561 tcgtaaaagc cgatggacgc atcgcgggat gcgcgctttc gatcatcgtg gattacgatt
     4621 cgtttgataa tcgccatacg tatcgtgaga ttaccgccga cttcaccttt aagacgcata
     4681 gcgcacaggg agacgttctt tacggtatcg atgtcttcgt ccatcccgat tttcgcggtc
     4741 tgaggctggg gcgtcggctt tacgattatc gcaaagaact ctgcgaacgt ctcaatctca
     4801 aagcaatcgt ttttggcggc aggataccca actaccataa gtatgcgtca gaattaagcc
     4861 cgaaggagta tatccagaaa gtacgcgaac gcaagataca cgatccggtg ttgaattttc
     4921 agatgtcgaa cgatttctat ccgtcgaaag tgttgcgcgg ataccttgaa ggcgaccagg
     4981 cttcgggtga gtatgccgtc ttgctgcggt gggacaacat ttattacgaa aaaccgtcta
     5041 caaagccggc cgcgaaaaag acggttgttc gcttggggct catccagtgg cagatgcggc
     5101 cttacaagaa cctcgacgat ctgatgaggc aggccgaata tttcgtggat gctttggcgg
     5161 cgtaccgggc agattttgcc ctcttccccg aattttttaa cgtaccgctc atggccgaac
     5221 aggaaaaaat gaacgaaccc gacgccattc gagcgcttgc cgaatacact ccggccatcg
     5281 cccgtcgctt tcaggagctt gctatcaggt ttaatatcaa catcatcacc ggcagcatgc
     5341 ccgacatcag cgacggtaaa ctcattaacc gcggattcct ttgtcgcagg gaaggaagca
     5401 ccgattccta tgaaaaactc catgtgactc ccgacgaagc aaaagtatgg ggcatggtgg
     5461 gaggaaacaa gctaacggca tttgacaccg attgtggcaa gatcggcatt ctcatttgtt
     5521 atgactctga atttcccgaa cttgctcgca tgcttgccga cgaaggtatg gaaattctct
     5581 ttgtaccgtt tttaaccgat acacaaaacg catattcgcg ggttcgtctc tgcacccaag
     5641 cgcgagccgt tgaaaatgaa tgctatgtcg ctatcgcagg caatgtcggt aatttgccga
     5701 atgtgcacaa catggatatt caatatgcac aatctgccgt gttcacgccg tgtgacttcg
     5761 cttttccctc aaatggtatt aaggccgaag caacggcgaa taccgagatg gttctggttg
     5821 cagacgtgga tctctcgctg ctgagagaat tacaccaatt gggcagcgtc cgcaacttgc
     5881 gcgaccgacg gcacgactta ttcgagctca aacgccgcga tcagataccc gtaaatacgc
     5941 agtgaggcgg tggttctcgg tggcatcgtc gaatacgtgc ggtatttcct gctgcgcgcg
     6001 cgaccactcc gttggtgggt gcgcaccgaa gacggagacc cggcaggtcg cggcacataa
     6061 agtgacggaa attggctcta cagaggtcaa agttggccca ggctttacca gacgtcagcg
     6121 taaattccac ggtggatagc atgacaccat ttttttagtt tggaaaaata att
//
LOCUS       DAOMPD010000106         6158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5442_length_6158_cov_2.651974, whole genome shotgun sequence.
ACCESSION   DAOMPD010000106 DAOMPD010000000
VERSION     DAOMPD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5442_length_6158_cov_2.651974"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLY93_05000"
     CDS             complement(<1..836)
                     /locus_tag="PLY93_05000"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRP68869.1"
                     /translation="MGSRVDDKRIAILSSGGDSPGMNAALRAFVRSALWEHYQVYGIH
                     NGYAGLLAGDINELSSRDVGLTIDKGGTFLGTARSPEFQTPEGLKKAAAVLTNKKIDA
                     LCVLGGDGSFRGLTALSQLYKGQLIGIPGTIDNDIPGTEYTIGFDTAVQTAVEAIDKL
                     RDTALSHGRIFFVEVMGRKSGQIALSVAIASGAEDVIIPEEKLDLDAFAHRLEAGRKR
                     GKRFGIIVVAEGAECTSRGDVHAQEVRAAAPDPTVKAALTVEESPAFHWAEVLRPRLG
                     MN"
     gene            complement(829..1647)
                     /locus_tag="PLY93_05005"
     CDS             complement(829..1647)
                     /locus_tag="PLY93_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRP68870.1"
                     /translation="MALELKKENRVALVKINHNPANTLTHDSFAAIADMLDELEKDES
                     VRVVVFTGNDSGYFSNGFEPSLFLKKSYAENLATCKLVNKTALQYFFFTKPMIACLNG
                     HAMGAGAVFALFSDWRMMLDASARLGFPEALLGMNFPAFVARYMQDLIGVQATRDILF
                     VGKSLKGPEALELGLVDNIFSEETLISETLKFADKLAKSPIQVLQGMKRARTENYVAH
                     FSALSNDDAAELARYVTSPITQEGLLSIVEKRRPRFEPKETKPSLHILKGGDNG"
     gene            complement(1673..3745)
                     /locus_tag="PLY93_05010"
     CDS             complement(1673..3745)
                     /locus_tag="PLY93_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRP68871.1"
                     /translation="MRTLLNQLKVRTKMLMAMGTILISLAYLTTQFYVETRRGLNTSR
                     LEMAGIEYITPLQQVLHHVQRRRGFAALHRPGIADKTQNYQQEGEAARKALADLLLVH
                     EQQNTLFDVGSAWLDFKSKLDFLLNAPLSVEKRKLIQLHTDTIQQVLNFQESISERSM
                     LDLNQNPESYYLIELSQKLVAPLSEGLGRLRASASAYFTSGARDNKALRQADALFQAN
                     VERAQGIVAIRQRVLEYNVERRNDLEKAYKQQNEAGIVFQKLHTRLITENQGGQGDAT
                     EYFEKVSAYIDTLHNFSSLANEIVENNLASHHRSLLIRQWTVLVLTTLGVLFSISMGL
                     YFLRHIGRSLDDALRLSNGLADGNLEQEVKVVGSDEIAVFMKSLQRMVTKLREVLQSV
                     HQSSAEISLASQQVASTAEMLNNGAMDQAAHVEETGAALGEMVNLIGSNANNAIETDK
                     TANVAMDNTKKGADNVMKAVESMRVISERIQIVQEIASQTNLLALNATIEAARAGEHG
                     RGFAVVATEVGKLADTSGQAAKQIQELLHESSRISETAASSLSLITDSMQNTAQKVVA
                     IRKASEEQDLAAKQINESMGRLNQTTEQTASAAEELAATAEEMSSQTSLLMENLKFFT
                     FKREEAEHPVLAGLKKSKELKAEKNKSSLKPSTPIPTAPQEKSSVTPSKKQSTDEFIA
                     STGEYEKF"
     gene            3828..4202
                     /locus_tag="PLY93_05015"
     CDS             3828..4202
                     /locus_tag="PLY93_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRP68872.1"
                     /translation="MQIVTTPHAPAAVGPYSQATKAGNMVFLSGQIPLDPQSGELILG
                     DITAQATRVLENLKAVCEATGGNLQNVVKCTVFMVDLAHFQAMNTVYAQYFKEHKPAR
                     STIQVAALPKGAQVEIEAIMVI"
     gene            4346..4828
                     /locus_tag="PLY93_05020"
     CDS             4346..4828
                     /locus_tag="PLY93_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRP68873.1"
                     /translation="MSIHEHREFVNVYQNFPEGMKAVLALEQACHSGPLDPFIYELVK
                     MRASQINGCAHCLDMHYKDARAMGETEERLYMLNAWRESKLYSPAERAALALTEEVTL
                     IAKHHVSAEVEAEARKHFDARTYANLLFTIATINVWNRLAIASHSPAGEYKSTKQKLK
                     "
     gene            4889..5206
                     /locus_tag="PLY93_05025"
     CDS             4889..5206
                     /locus_tag="PLY93_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRP68874.1"
                     /translation="MSWIYLFLGSFFEIGWPLGFKLSQTTEYKITSIAFAIVSMFLSG
                     IFLWLAQRTIPIGTAYAVWTGIGAAGTFIVGIAFFNDAASMMRIFSVLLILAGVIGLK
                     LAG"
     gene            complement(5231..5737)
                     /locus_tag="PLY93_05030"
     CDS             complement(5231..5737)
                     /locus_tag="PLY93_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRP68875.1"
                     /translation="MNTNPILLKVHRSPKHGRGVYTEVAIPKGMKIIEYVGKKIPVRK
                     SDVVAEKQLSRAAKHGAGSVYLFELNKKFDIDGNVPWNKARLINHSCDPNCEVEIVQG
                     HIWISSRRAIKAGEELTYDYGFNMANWQEHVCRCGTKKCLGYIVSASRRGALARVLKR
                     AARASKKR"
     gene            5887..>6158
                     /locus_tag="PLY93_05035"
     CDS             5887..>6158
                     /locus_tag="PLY93_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRP68876.1"
                     /translation="MKAYLLENSFGIENIHLVEQEVAELSAHEIRVAIKTVSLNFRDY
                     LMMTGNYNPRLKLPLIPLSDGAGEVVAVGERVTRFKVGDRVMPSFS"
BASE COUNT         1507 a         1575 c         1387 g         1689 t
ORIGIN      
        1 atgttcatac ccaagcgtgg acgcaacacc tctgcccaat ggaatgcagg cgattcttcc
       61 accgtgagcg cagcctttac ggttgggtcg ggtgcggctg cacgcacttc ttgtgcatgc
      121 acgtctccac gcgacgtaca ctctgcgccc tcggcgacaa cgatgatacc aaaacgttta
      181 ccgcgcttac gccctgcttc cagacggtgg gcgaacgcat cgaggtcaag tttttcctcg
      241 gggatgatga catcttctgc acccgaggca atcgccaccg acaacgcaat ttgtcccgat
      301 ttacgaccca tcacttcgac aaaaaaaatg cgcccatgcg aaagcgcggt gtcgcgtagt
      361 ttatcaatcg cctcgacagc ggtttgtact gccgtgtcaa aaccaatcgt gtactcagtg
      421 ccggggatgt cgttatcaat cgtgccggga attccaatga gttgaccttt ataaagttgc
      481 gacaacgccg taagcccacg aaaggagcca tcccccccca aaacacacag cgcatcgatt
      541 tttttattcg ttaaaacagc ggcggctttt tttaacccct cgggagtttg aaattcggga
      601 gagcgagcag ttcccaaaaa cgtaccgcct ttgtcgatgg tcaatcccac atcgcgcgaa
      661 gagagctcgt tgatatcacc cgctaagagt ccagcataac cattatgaat tccatacact
      721 tggtagtgct cccacaacgc cgagcggacg aacgcacgca acgcggcgtt catgccgggg
      781 gagtcgccgc ccgaggaaag aatggcgatg cgcttatcgt cgacacgact acccattgtc
      841 gccacctttc aaaatatgca gcgacggctt tgtttctttc ggttcaaagc ggggacgtct
      901 tttttccaca attgatagca acccttcttg ggtaatcgga ctcgtgacgt aacgcgccaa
      961 ctctgcggcg tcgtcgttac tcagcgcaga aaaatgcgcc acatagtttt ctgtgcgtgc
     1021 gcgtttcatg ccttgcaaca cttggatggg actcttcgct aatttatctg caaatttaag
     1081 cgtctcggaa atgagtgttt cctctgaaaa aatgttatcc acaagaccaa gttcgagagc
     1141 ttccggtcct ttgagcgatt tccccacaaa gagaatatcg cgcgtggctt gcacaccgat
     1201 gaggtcttgc atatagcggg cgacaaacgc aggaaagttc atccccaaga gcgcttcggg
     1261 gaaacccaac cgcgccgacg cgtcgagcat catgcgccag tcggaaaaaa gcgcgaacac
     1321 cgcccctgct cccatcgcat gcccattgag gcaggcaatc atcggtttgg taaagaaaaa
     1381 atactggagc gctgttttat tcaccagctt acacgtcgcc aaattttctg catacgattt
     1441 ttttaagaaa agcgacggtt caaacccatt cgagaaatag cccgagtcgt tgccggtgaa
     1501 tacaaccacc cgcacactct cgtccttttc cagttcgtcg agcatgtccg caatcgccgc
     1561 aaagctatcg tgcgtgagcg tgttcgctgg gttatgattt attttaacga gcgcaacgcg
     1621 gttttccttt tttaattcga gcgccatctt tctacctttg ttttttttat tctcaaaatt
     1681 tttcgtattc gccggtcgaa gcaataaact catcggtcga ttgctttttg ctgggcgtta
     1741 cgctactttt ttcctgcggc gcggttggta ttggcgtcga cggtttcaag gaagatttat
     1801 tcttttctgc ttttaattcc tttgattttt ttaatcccgc caataccgga tgctctgctt
     1861 cttcgcgctt gaaagtaaag aatttcaaat tttccataag caacgaagtt tgcgaactca
     1921 tttcctcggc ggtggccgcc aactcttctg ccgccgatgc agtttgttcg gtggtttggt
     1981 ttaaacgccc catgctttcg ttgatttgct ttgccgccaa atcttgctct tcggacgctt
     2041 tacgaatcgc gaccactttt tgtgcggtgt tttgcattga gtcggtgata agcgaaagcg
     2101 aagacgctgc ggtttctgaa atacgcgagc tttcgtgcaa gagttcctga atttgcttgg
     2161 ccgcctgacc gctcgtgtcg gcaagcttgc ccacctcggt cgcaacaacg gcaaaaccgc
     2221 ggccatgctc gcccgcacgc gccgcctcaa tagtggcatt gagtgcaagt aaatttgtct
     2281 gcgatgcaat ttcttgcaca atttgaatac gctccgaaat gacgcgcatc gactctaccg
     2341 ccttcatcac attatctgca cctttcttcg tgttgtccat cgccacgttg gcggtcttgt
     2401 cggtttcgat ggcgttgttg gcattactgc cgatgagatt aaccatttct cccaacgccg
     2461 cgccggtttc ctcgacgtgc gccgcttgat ccatggcgcc attgttgagc atttcggcgg
     2521 tgctcgccac ctgttgtgaa gcgagtgaaa tttctgccga gctttggtgt accgattgca
     2581 acacctccct gagttttgtt accatccttt gtaacgactt catgaagact gcaatttcat
     2641 cgctccctac aactttaact tcttgctcca agttaccatc ggcaagtcca ttcgacaagc
     2701 gtagcgcatc gtcgagtgaa cgcccaatgt ggcgcaaaaa ataaagtccc attgaaatag
     2761 agaataaaac ccccagcgtg gttaaaacga gcactgtcca ttgacggatc aacaacgaac
     2821 ggtggtgcga tgcaaggttg ttctcaacta tttcattcgc caaactgcta aagttatgta
     2881 aagtatcgat atacgcactg accttctcga aatactcagt ggcgtcgcct tgaccgcctt
     2941 ggttctcggt aataaggcgg gtatgtaatt tttgaaaaac aatacccgct tcattttgtt
     3001 gcttatacgc tttctcaagg tcgttacgcc tttcaacatt atactccagt actcgttgcc
     3061 gaatcgccac aatgccttgt gcacgctcta catttgcttg aaataaagcg tcagcttgac
     3121 gaagcgcttt attgtcgcgt gcgccacttg taaaatacgc cgaagcgctt gcacgtagtc
     3181 gccccaatcc ttcgctcaac ggtgcgacta atttttgaga tagctctatt aaataatacg
     3241 attcgggatt ttggtttaag tctaacatcg acctttcaga tatagactct tggaaattca
     3301 acacctgttg aatggtgtcg gtgtggagct ggatgagctt acgcttttct accgatagag
     3361 gtgcgttgag aagaaaatcc aacttacttt taaaatcgag ccacgcactt cctacatcaa
     3421 agagtgtatt ttgttgttcg tgcacaagca gtaaatcggc caaagcttta cgtgcagctt
     3481 cgccctcttg ttggtaattt tgtgttttat ctgcaatgcc tggtcgatgc aacgccgcaa
     3541 aaccgcgccg ccgctgcacg tggtggagaa cttgttgcaa aggagtaatg tattcaatac
     3601 cagccatctc cagacgcgac gtgtttagac cgcgccgcgt ttcaacataa aattgggtcg
     3661 tcaaataagc taacgaaatg agaattgtcc ccattgccat caacattttc gtgcgtactt
     3721 ttaactgatt taagagagtt ctcattattc tattttcgac cttttaaacg ttatagtaaa
     3781 cttttaattc attgacgaca ctttttcatg ccatttatcg tctctgtatg caaattgtca
     3841 caacgcctca cgcccctgca gcggtcgggc cttactcgca agccactaaa gcaggtaaca
     3901 tggtttttct ctccggccaa ataccgctcg acccacaatc gggtgaactc atcctagggg
     3961 atatcacagc gcaggccacc cgtgttcttg aaaacttgaa ggcagtgtgc gaagcgactg
     4021 ggggtaatct tcaaaacgtg gtaaaatgca ctgtttttat ggtggatcta gcgcacttcc
     4081 aagcgatgaa cactgtgtac gctcaatact tcaaagagca caagcctgca cgctctacca
     4141 tccaagtggc tgcgctccct aaaggtgcgc aagtagaaat tgaagcgatc atggtgattt
     4201 aagataatgt agaggcagct aatagcctgt gcgccaactg gctgagacat ttttttgtaa
     4261 agaagatttt ttttaatttt ttgtggtcgt tgaaataaag aatgtttact cttttattag
     4321 atgaacttta tataagaggg aataaatgtc aatccatgaa caccgcgaat ttgtgaacgt
     4381 ctaccaaaat ttccccgaag ggatgaaagc agtcttggct ttagaacagg cttgccactc
     4441 ggggccgctc gatccgttta tttacgaact tgtgaaaatg cgcgcttcac aaatcaacgg
     4501 ctgcgcacac tgcctcgata tgcactataa agacgcgcgc gcgatgggcg aaaccgaaga
     4561 gcggctttat atgctgaacg catggcgcga atcgaaactc tactcgcctg ctgaacgtgc
     4621 cgcattggct ctcaccgaag aagtgactct cattgcaaag caccatgtat cggcagaggt
     4681 agaagcagag gcgcgtaaac atttcgacgc acggacgtat gcaaatttac ttttcacgat
     4741 tgcaacaatc aacgtatgga accgcctcgc aatcgcctcg cattcgccag caggcgaata
     4801 taaatcgacc aagcaaaaat taaagtaaac acaaagtgta cgcttcattg ttgcttgtat
     4861 cgctattggc tatactcaaa gttacaccat gagttggatc tatctctttt taggctcctt
     4921 ttttgaaatc ggttggccgt tgggctttaa gctctcgcaa acgactgaat ataaaatcac
     4981 gagtattgca ttcgctattg tatcgatgtt cctcagcggt atttttttat ggctcgcgca
     5041 acgcaccatc cccatcggca ctgcgtacgc ggtgtggacg ggcatcggcg ctgcaggcac
     5101 gtttattgta ggcatcgcct tttttaacga cgccgcaagc atgatgcgga ttttttcggt
     5161 gctacttatt ctggccggcg ttatcggctt aaaacttgcg ggatgaagtt atttcactat
     5221 taagaataat ctatcgtttt tttgaggcac gcgccgctcg cttcaacacc cgcgccaaag
     5281 cgccccgtcg cgaagccgat acaatataac ccagacactt ttttgtgccg cagcgacaaa
     5341 cgtgctcttg ccagttagcc atgttgaatc catagtcgta tgtcaattct tcgcccgctt
     5401 taatcgcccg acggctggaa atccaaatgt gaccctgcac aatttcaacc tcacagttgg
     5461 ggtcgcacga gtggttaatg agacgcgcct tattccaagg gacattcccg tcgatgtcga
     5521 actttttatt caactcgaat agatacaccg agcccgcgcc atgctttgcc gcacgcgaaa
     5581 gttgtttctc ggcaacgacg tcgctttttc tcacggggat ttttttaccc acatattcga
     5641 taattttcat acctttgggg attgccactt ctgtataaac accacgccca tgcttggggg
     5701 aacgatgtac tttcaataaa attggatttg tgttcataag actctatacg gttaagggaa
     5761 ttaaaacttt tacgacgatc atcacaaagg ctgaaagtta ggcatttttt tgtcactacg
     5821 cgtcaacgag aattaaacga ttataaatct tctcgacggg tggtttcttt ctttgatagc
     5881 acgactatga aagcatatct ccttgaaaac tcattcggca ttgagaatat ccatttggtc
     5941 gagcaagaag tagccgaatt atctgcacac gaaatccgtg tagcaattaa aacagtctca
     6001 ctcaactttc gcgattatct tatgatgaca gggaactata acccccgcct taaattacct
     6061 cttattccct tatccgacgg cgccggtgaa gtcgttgcgg tgggcgagcg cgtcacgcgc
     6121 ttcaaagtcg gcgacagggt gatgccctct ttctcaca
//
LOCUS       DAOMPD010000107         6153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5447_length_6153_cov_3.390784, whole genome shotgun sequence.
ACCESSION   DAOMPD010000107 DAOMPD010000000
VERSION     DAOMPD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_6153_cov_3.390784"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    215..224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <225..952
                     /locus_tag="PLY93_05040"
     CDS             <225..952
                     /locus_tag="PLY93_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRP68877.1"
                     /translation="DLLFTAQTKDNMPLNLYILLEHKSKPDKNILTQLLIYQKEIYVK
                     QEKYAAVIPVVFYHGKEKWNIPKKFAEPHERLIKYALNFRYILINLSEYNFEKLKISL
                     TARAILGIFQKIWYLEDEEIFKDYIRAIADLFSAENNAALLQKIFVYLYSVHTIEPIE
                     TRKRIEAVIAEKGDVAMSTAELLIERGIEKGIEKGIERGVEKNKLETARRMLDKGLSL
                     NDILEITALSLDDLRAAGLISAD"
     gene            1205..1864
                     /locus_tag="PLY93_05045"
     CDS             1205..1864
                     /locus_tag="PLY93_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRP68878.1"
                     /translation="MQNNGTKKKLKEAMATSETIRMFKWDWMERLSHVHPATPFVIYI
                     PVVVVTMYFAFLTPNTTIALTLAYFLLGMLFWTLIEYILHRFLFHIPQTNKVFKAIYF
                     YSHGIHHDAPNDATRLVMPPSASVPLAILFFYAFKAWTGTYYLTLFAGFLTAYMIYDF
                     IHFATHFINFKATWFRKVKENHMRHHFLSSKHNFGLSSPLWDYVFFTLFRGKKSIEEK
                     S"
     gene            1989..3158
                     /locus_tag="PLY93_05050"
     CDS             1989..3158
                     /locus_tag="PLY93_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68879.1"
                     /translation="MLLNMTRTIKVVTVLAIASTSWLIAQDLQLPAIDAADKAAQDAE
                     KKVNEAERATDGSAAPARVEQGATTATAPNSNATADNQVGHIYNDGKNTYATSQARFE
                     LTATDNVSNLDFIEYKIDEANQFSIYGHPFGIEKEGPHKIVYRSVDRVGNREEDNIFN
                     VIIDNTGPYLNFALNKAPVVIDGKRYLAPGTKLEIRATDNYSGVKSLEYSTNGQDWQS
                     YKDGIILDKAGAASLKYRAVDNLGNRSENLSGFSTAAAQQGASPEDLAIINSLYVENT
                     PPVVLITPEKRWITVDGKKYARRDNSYLVEAQDAESGVANIFVKVDNSAEWQIYSGKL
                     VFSTDGPHSIEAKAVDKVGNESQPVKVDFIVDDNPPKSTIRPVGGNTAPAPDAAQ"
     gene            3121..3825
                     /locus_tag="PLY93_05055"
     CDS             3121..3825
                     /locus_tag="PLY93_05055"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HRP68880.1"
                     /translation="METLPQHQTLHNKLHRFYKKSSARIAGLFFYFFLWITLSAISPV
                     AAPKFSADHQIYFRRFLEVFTPLVLKHEKQVGNPRWGSKNKDCAGLVRYLFWEAMQRH
                     DERFYRLYPSMHSVTLSSPTHSFAEFSKAWTERNATSSDLLRHAFYLGKERKNLQLKT
                     GDFLYYESARLNIRHVMLVIRVEQTPYLIYHTGGVPSELRIRTFADLDALPQSQWHAR
                     ATNHVFRGFYRATFLD"
     assembly_gap    3939..3948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3969..>4395
                     /locus_tag="PLY93_05060"
     CDS             3969..>4395
                     /locus_tag="PLY93_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68881.1"
                     /translation="MRIGKAALAALQEAYIKHDAKAFVAVLSKEALEDLDEKIDSMRA
                     MFQSMPENPQAAKAFEHMAKEMGLPVAKLKELTVEDFIGYMMQTENASGSETTLLPKE
                     MLPPAQVIRRIEKGNKATLYFGDDKKLSFIKTDAGWRVYL"
     assembly_gap    4396..4405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4472..5014)
                     /gene="hslV"
                     /locus_tag="PLY93_05065"
     CDS             complement(4472..5014)
                     /gene="hslV"
                     /locus_tag="PLY93_05065"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRP68882.1"
                     /translation="MTIHATTILCIKRDGKTAMAGDGQVSAGQTILKGNAVKVRKLFS
                     GKILAGFAGATADAFTLLEKLEEKLQAFGGNLTRAAVELAKDWRTDKMLRRLEAMLIA
                     ADNEKIFLLTGNGDVIEPDEGVVAIGSGGNYALSSARALLRHTNLSAEEICKESLKVA
                     AEICVYTNNSITLESLPNNQ"
     gene            complement(5064..6071)
                     /locus_tag="PLY93_05070"
     CDS             complement(5064..6071)
                     /locus_tag="PLY93_05070"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HRP68883.1"
                     /translation="MKTKTSKAAIIQESEFKPQRACLPKPGDSHYFGLYEGPIDDTTT
                     REWDFPTRIATRRRLERKAWIYLGFFSRDFYMGLAIADAGYIGKAFSYVYIPSEKFFM
                     EDKITQPLAFPGDFDATLNNSWRMGRYEILPSNDRITVNFRGTFDLTATFQRKHNGLS
                     TIAPVDNRPFNFTYKEMELLTEGEFIFGSRRQKFSGACGVLDYTKGYPARQTHWNWLS
                     MVGKTETGKNLSVNLVDKFNESIENALWLDGRAMSLSSAHFHFLRPADQSIWRILTRD
                     NIFETRFRPFGARGENLNLGVLRSKFIQPYGIFDGQVQVGDTTEKFTGYGVTEDHLAL
                     W"
BASE COUNT         1730 a         1412 c         1366 g         1615 t
ORIGIN      
        1 atgaaatcat ttgacgcaac gtaacacacg gcggacgcaa ccccgtggac atcatcaacc
       61 atccacacgc taactttgcc gcatctatct tcaaagagac tgaatacgcc gtcgctatct
      121 taaaaccgct cctacctccc gaggtaagcg agagtatcga ctggcaaacg ctcgctctca
      181 ccgacggctc gtttgtcgat gagaaactca agacnnnnnn nnnngcgatt tactttttac
      241 cgcgcagacg aaagacaaca tgccgctaaa tttatacatt ctgcttgaac ataaatccaa
      301 acccgataaa aatattttga ctcagcttct gatttatcaa aaagaaattt atgtaaagca
      361 agaaaaatac gccgccgtca tccccgtggt tttttatcat ggcaaagaaa aatggaatat
      421 acccaaaaaa ttcgccgaac cgcacgaacg gctcatcaaa tatgcgctta attttcgcta
      481 catcttgatc aacttgtccg aatataattt tgaaaaattg aaaatttcat tgacggcaag
      541 ggcaatcttg ggtatctttc aaaaaatatg gtatcttgaa gatgaagaaa ttttcaagga
      601 ttatattcga gcgattgcgg atttgttttc ggcagaaaat aacgccgctc tgcttcaaaa
      661 gattttcgtg tatctctatt cggtacatac gattgaaccg atagaaacac gaaagcgtat
      721 tgaagcggtt atcgctgaaa agggggacgt tgcaatgagc actgcagaac ttctaattga
      781 acgcgggatt gaaaagggta tcgaaaaggg tatcgagcgg ggtgttgaga agaacaaact
      841 cgaaactgcg cgccgtatgc tggataaagg tctgtccctc aatgacattc ttgaaattac
      901 agcgctctcc ctcgacgatc tccgcgctgc cggacttatc agcgcagatt gagcttttat
      961 agttgaagtc ccccccgaag agcgactctt tgcggtagat ctcgtcgcgc agtggatgcg
     1021 gggtcgcgat gtcgaacctg tgcgcgcagc gtccacaaac gcttgcaaaa aaattgtctc
     1081 tttgtacacg cgtccaaatt ggcgtacagg cttgaaggta ataactaatc cttgacgcgg
     1141 ggaataaaac tttgcttctt tacacaagta ataatggaga acctttgttt ctccacgagg
     1201 gaagatgcaa aataacggca ccaaaaaaaa acttaaagaa gccatggcga cgagcgaaac
     1261 aatccgcatg ttcaaatggg attggatgga acgcctttcg cacgtacatc ccgcaacgcc
     1321 gtttgtgatt tatatccccg tcgtggtggt aacaatgtat tttgcatttt taactcccaa
     1381 caccacgatt gcgctcactc tcgcctattt ccttttggga atgttattct ggacactcat
     1441 cgagtatatt ttacaccgtt tcctttttca catcccccaa accaataaag ttttcaaagc
     1501 aatctatttt tattcgcatg gaatccatca tgacgccccc aacgatgcca cacgccttgt
     1561 catgccaccg agcgccagtg tgcctttagc aattcttttt ttctatgctt tcaaagcatg
     1621 gacaggcacg tattacttaa cgctctttgc cggctttttg acggcgtata tgatttacga
     1681 ctttattcat tttgccacgc attttattaa ttttaaagca acgtggtttc gcaaagtaaa
     1741 agagaatcat atgcgtcacc attttctatc ctccaaacat aattttggtt taagctcccc
     1801 attgtgggat tatgtcttct ttactctctt tcgtggaaag aaaagcattg aagaaaaatc
     1861 ataaaataaa ttgacattgt gtgcttgata tagggcactg agttaagtct tcgactataa
     1921 agggcgatac ttaaataccc ttaggttgaa ttgcaacgat taatgattga tttaaaagag
     1981 aggaaaaaat gttactgaat atgacacgca ccattaaagt ggtaactgtg ctggccattg
     2041 catctacatc atggctcatt gcgcaagatt tacaattacc cgcgattgac gctgcagata
     2101 aagcggcaca agacgcagaa aagaaagtca acgaagccga acgcgccacc gatggctcag
     2161 cggcgccagc gagagtagag caaggtgcaa ccactgcaac tgcacccaac tcaaacgcca
     2221 ctgccgacaa ccaagtgggt catatttaca acgatggtaa aaatacatac gcgacttcgc
     2281 aagcgcgctt tgaattgacg gcaaccgaca atgtttcaaa cttagatttt attgaatata
     2341 aaatcgacga agcgaatcaa tttagtattt atggccatcc ttttggtatt gagaaagaag
     2401 gtccgcacaa aatcgtgtat cgttccgtcg accgtgtcgg taaccgtgaa gaagataata
     2461 tcttcaacgt cattattgac aacactggcc catatctgaa cttcgcgctt aacaaagccc
     2521 ccgttgttat cgatggcaaa cgctatttag cgcctggcac aaaattagaa attcgtgcaa
     2581 ccgacaacta cagcggtgta aaatcactcg aatactcaac caatggacaa gactggcaat
     2641 cgtacaaaga cgggattatt ctcgataaag ccggtgcagc aagcctcaaa taccgcgccg
     2701 tcgataattt gggtaatcgt tcagaaaatt taagcggctt ttcaacagcg gctgcacaac
     2761 aaggagccag cccagaagac ttggcgatta tcaactctct ttatgtggaa aacactcccc
     2821 ctgttgtgtt gattactcct gaaaaacgct ggattaccgt cgatggcaaa aaatacgcac
     2881 gccgcgacaa cagctactta gttgaagcgc aagatgctga gtcaggggta gcgaacattt
     2941 tcgtgaaagt ggacaacagc gcagaatggc aaatttacag cggtaaactt gtcttctcaa
     3001 cagacggacc gcactcgatt gaagcgaaag ctgtcgacaa agtgggtaat gagtcacaac
     3061 cagtgaaagt agatttcatt gtagacgaca atccacccaa atcgacaatc cgtcctgtgg
     3121 gtggaaacac tgccccagca ccagacgctg cacaataagt tgcatcgttt ctataaaaaa
     3181 agctctgcaa gaattgcggg gctttttttt tacttttttc tttggattac attaagtgcg
     3241 atttcccccg ttgccgcgcc aaaattttca gcagaccatc aaatttattt ccgccgtttt
     3301 ctcgaagtgt ttaccccttt ggtcctgaaa cacgaaaagc aagtgggcaa tcctcggtgg
     3361 gggagtaaaa ataaagattg tgcaggcttg gtacgttatc ttttttggga agcgatgcaa
     3421 cggcatgatg aaaggtttta ccgcctttac ccttcgatgc actcggtaac tctttcttcg
     3481 ccgacgcatt catttgccga atttagtaaa gcgtggacgg aacgcaatgc aacatcgagc
     3541 gatctattgc gccacgcctt ttatttaggt aaggaacgca aaaatcttca actgaaaacg
     3601 ggcgattttc tttattatga gtcggcacgc ttgaatatac gccacgttat gctcgttatt
     3661 cgcgtcgagc aaacaccgta tttaatttac cacacgggtg gtgtgccaag tgaattgcgc
     3721 atccgtactt tcgccgattt agacgcgttg ccacaaagcc agtggcatgc gcgtgcgaca
     3781 aatcatgtct ttcgcggctt ctaccgtgcc acttttttgg attaaccgat tgtcgctctg
     3841 actcgccgat ttaagattga gtatggcacg cgcttttatt gcaatagtta cccttgtttc
     3901 tttttttgct tgcggcaaaa aaaagttaga gaccccagnn nnnnnnnncg atgggcgggg
     3961 ggccggtcat gaggatcggg aaggcggcat tggccgcgct gcaagaagcg tatattaagc
     4021 acgacgcaaa agcttttgtc gcagttttat cgaaggaagc attagaagat ttagatgaaa
     4081 agattgattc gatgcgtgcg atgttccagt caatgccaga aaatccacaa gcggccaaag
     4141 cgtttgaaca catggcaaaa gaaatgggtt tgccggtcgc taaactcaaa gagctcaccg
     4201 ttgaagattt tatcggttac atgatgcaga ctgaaaatgc atcgggcagt gaaacaacgc
     4261 ttctgcctaa agaaatgttg ccgccagcgc aagtcatccg ccgtatcgaa aaaggcaata
     4321 aagccactct ctattttggc gacgacaaaa agctttcgtt tataaaaact gatgcggggt
     4381 ggcgggtgta cttaannnnn nnnnncaaag aaagaaattg cccgccgttg atggaaacga
     4441 cgacggagaa taaacataat aaaatagtat tttattgatt gtttggtagc gattccaatg
     4501 taatggaatt gttcgtgtaa acacaaattt cagcggccac tttgagcgac tctttgcaga
     4561 tttcttcagc actcaaattt gtgtgccgca acaaggcgcg cgccgaagag agagcatagt
     4621 taccccccga gccaatcgcc acaacgcctt catcgggctc gatcacgtcg ccgttgccgg
     4681 tgagcagaaa aatcttttcg ttatcggcag cgataagcat cgcttctagg cggcgcaaca
     4741 ttttgtcggt acgccaatct ttcgccagtt caacagcggc gcgtgtcaaa ttgccaccga
     4801 acgcttgtag tttctcctcg agtttttcta acaaagtaaa tgcgtcggca gtggcgccgg
     4861 caaagcctgc taaaatttta cctgaaaaca gcttacgtac tttaactgcg ttgcctttga
     4921 ggatggtttg tcccgcgctg acttgaccgt cccccgccat cgcggtttta ccgtcgcgtt
     4981 taatgcataa aattgtggtc gcatggattg tcatagaatt ccttcattaa atagttgaaa
     5041 atagttaata acgtcctgcc tcgtcaccag agggccaaat ggtcttctgt tacaccgtac
     5101 cctgtaaatt tttcggtggt gtctcccact tgcacttgac cgtcaaaaat tccatacggc
     5161 tgaatgaatt tgctgcgcaa cacacccaaa tttaagtttt cgccgcgcgc accaaaaggc
     5221 cgaaagcggg tttcgaatat attgtcgcgc gttaaaatac gccaaataga ttgatcggcg
     5281 ggacgcaaaa agtgaaagtg cgcgctactc aaactcattg cacggccatc gagccacagc
     5341 gcgttttcaa tgctttcgtt aaatttatcg accaaattta ccgataaatt tttacccgtt
     5401 tcagttttgc ccaccattga aagccagttc caatgcgttt ggcgtgcggg gtatcctttg
     5461 gtgtaatcca gaacgccaca agcgccggag aatttttgtc gccgcgaacc gaaaataaac
     5521 tcaccctcgg tgagcaattc catttcttta taggtgaaat taaacgggcg gttatcgacg
     5581 ggggcaatcg tgcttaaacc gttgtgctta cgttggaaag ttgccgttaa atcgaaagtt
     5641 ccgcgaaagt tgacggtgat gcgatcgttg gaagggagta tttcgtaacg ccccatgcgc
     5701 cagctattgt tgagcgttgc gtcaaaatca ccgggaaacg cgagtggttg cgtaatttta
     5761 tcttccatga aaaacttttc agagggaata taaacgtagc tgaacgcttt accaatgtaa
     5821 cctgcatcgg cgatggcaag ccccatgtaa aaatcgcggc taaaaaagcc aaggtatatc
     5881 cacgctttgc gttcgagccg cctgcgggtg gcgatgcgtg ttggaaaatc ccactcgcgg
     5941 gttgttgtat cgtcgatggg gccttcgtaa agaccgaaat aatgcgagtc gccgggtttg
     6001 ggtaaacaag cgcgctgcgg cttaaactcg ctttcttgta ttattgctgc tttagaagtt
     6061 ttcgttttca aagcttctcc tcaaagtgtt tacttcaaac cgtagttttg agatgccgcg
     6121 tcaacgaggt agttatccaa tcttaacgtc tgt
//
LOCUS       DAOMPD010000108         6147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5452_length_6147_cov_2.674655, whole genome shotgun sequence.
ACCESSION   DAOMPD010000108 DAOMPD010000000
VERSION     DAOMPD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_6147_cov_2.674655"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(564..842)
                     /locus_tag="PLY93_05075"
     CDS             complement(564..842)
                     /locus_tag="PLY93_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68884.1"
                     /translation="MRAQTNQKLGVVGQRGQVTLDSKLAGRSVRITKIDTGRYLVEEM
                     SMIPASEAWTHKPKNKKIITKGIESLKKLSKKSIKNTSDLDNFLKNVR"
     gene            complement(997..1416)
                     /locus_tag="PLY93_05080"
     CDS             complement(997..1416)
                     /locus_tag="PLY93_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP68885.1"
                     /translation="MRIVIVGAALAGPTAAARAREVNEKAEIILLERNTRVSYAMAGL
                     AHHLSGEVSSLDELNRERENFFRAVYNIDVRIRTEVVDIQTKQKLLTLKENGKQESLS
                     YDKLIYAAGAASLHPVGAPKDAENFRYFRTLESCSLT"
     gene            complement(1532..>2363)
                     /locus_tag="PLY93_05085"
     CDS             complement(1532..>2363)
                     /locus_tag="PLY93_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68886.1"
                     /translation="RGLFIVLATGVLWCGYKTVSCVTGYFRTQVVVYKVIDKKWKIIV
                     SLEQKSIETFTAWKDNVPRGAQIIKKMRKVRNYRDVASGTRTEKYDEKIEVATTREQQ
                     VTTTERVQSGEREECNTTYESVGSGAARKITRCRKVPVYMERNVTKKQNVPVTEEKMV
                     TKEREVTNYVQEAVYDTEVTYRAWVWNTVRSFTNEGNKLPIRQPQIILAPRERTAFYR
                     LTKPSVLFEVAIQRQGESDAPESTSVFTLEEEEFMQYRVGGLVDFLYTPAGQKLERKK
                     "
     assembly_gap    2364..2373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2374..2752)
                     /locus_tag="PLY93_05090"
     CDS             complement(<2374..2752)
                     /locus_tag="PLY93_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68887.1"
                     /translation="MNERVTIEYVYEYWDCLACGKKGIRGDHRFCPACGKARDANVQF
                     YRKRDAEEIIRDDNTRERLSTGADWICNFCSSANNIHDVFCPSCGAKSDASGKPVQRV
                     GDKPKTPKRNLSSKNSQEKKKKLP"
     gene            complement(2816..3553)
                     /locus_tag="PLY93_05095"
     CDS             complement(2816..3553)
                     /locus_tag="PLY93_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRP68888.1"
                     /translation="MRYHKILLACFLLTTAAVHAEAEEAVVTADVLRVRESTDLKAKS
                     LFTLKRGTIVRILSKSTTEMTIDGITASWFQIESEGKKGWSFGGFMSEEFQKLSGGNL
                     AWKIYPPNAPYEEGLIIVLFNTKTKKEQIVKTNIETADFKFSENLRYLALDQGTDVSG
                     GIQIIDLANKAAILVNDSYLPRHLLWKGNSLSFDKILCFDDGFGITEEIVFDNGKVRK
                     TKVYSKTDYHVGVTKGECQKFLHLIEK"
     gene            complement(3846..4226)
                     /locus_tag="PLY93_05100"
     CDS             complement(3846..4226)
                     /locus_tag="PLY93_05100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRP68889.1"
                     /translation="MKKSRITSGFLMGWSGVATPLTTIPSITTFLMVQYEVQYGDWVA
                     VKTWAASNGYTIINAGVQGNDGVGKTDQHPVTTISWRDAIVWCNAARYMSAGVSMRSD
                     DPRVAAAAICSSTTQGGRLTRPTF"
     gene            complement(4974..5579)
                     /locus_tag="PLY93_05105"
     CDS             complement(4974..5579)
                     /locus_tag="PLY93_05105"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP68890.1"
                     /translation="MAYRRMIGANASEDRLEKLIEDRLKPGADKQKIDARIWDLFGES
                     WAVMFTDLSGFSRNVAKFGIIHFLQTIFESERLLIPVIEDHDGILLKAEGDSLLVIFR
                     NVTKALQASVSMQRTLLAYNKDKLDEEKVLLCVGLGYGKMLRIGDVDVFGHEVNSASK
                     LGEDTAEAYEILVTPALHEAAQQDKDFTFESVDAHFRLKWQ"
     gene            complement(5665..>6147)
                     /locus_tag="PLY93_05110"
     CDS             complement(5665..>6147)
                     /locus_tag="PLY93_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68891.1"
                     /translation="AHPSMGFQSAIGVGRFYVGMAMDFRFGNSAEEYRYYDLNTKVLT
                     ATNNFFGVYIAADIRWDFLHIGDASLMLAGAFGYDLISHRSVPRYSGLRPSFSDSFNI
                     NGGLVFRQYFSASRNGFAEIDVRAHKVQFTDGGLGGDDLSGYYFTVLAGAGYRISFDD
                     "
BASE COUNT         1579 a         1509 c         1354 g         1695 t
ORIGIN      
        1 agatcccttc gtcgcacaaa cgggacgttt gttttgtgct cctcagggag gcgctccggg
       61 cacatttggt gacgcaaaat ggccttacaa tctgaattaa tgacataggc gtcaccaaac
      121 gtcaggtaaa ttccacgttc tctgacatag ttgcaccttt gtgatttgcc gccatccgtg
      181 gcggcaccgt gatacaagtc aaagatttgg atacagaaac ctatgaatcg tatacttctg
      241 cttttgaccg atctttattt taagcataag ccgaattatg atcaggatgc aaggaaataa
      301 aaaccaagtc atattctttc cgcatcacta tagctcgaaa tttctgattt aaccgaattg
      361 aatatggtcg atacttctta ttgcccaaga cgatatctgg catggaggtt atttcctccc
      421 acttcaaacc atgatcctga taaagctgat tccatgaaag ttgaagtacc ttccgaagcg
      481 ttttctgaaa ggatatcaga tcttctttgc tcagatttgc tatttgagag actaaggccg
      541 gtgaggacag gttaatagaa atcttatctg acatttttca gaaaattatc gaggtcggat
      601 gtatttttaa ttgatttttt actgagcttt ttcaaggatt caataccttt agtgattatc
      661 ttcttgtttt tcggcttgtg agtccaggcc tcagaggctg gaatcataga catctcttcg
      721 acgagatacc gtcccgtatc aattttagtg atccgaacag agcggccggc tagtttgctg
      781 tcaagtgtaa cctgccccct ctggccgacg acgccgagct tttgattagt ttgagcacgc
      841 atggtatgaa gatatgaaaa tcatactcaa ctgtcaatta tttttcagaa aaaccctcgc
      901 tgatttggct taaaaggtat gactttcacg aagaagaccg catccgagct attgttctta
      961 gtcgcccggc gtccatgccg ggctcacaag gtgcaactac gtcagagaac aagattcgag
     1021 agtacgaaag tagcgaaagt tctccgcgtc ttttggagcg ccgacagggt gaagcgacgc
     1081 ggcccctgcc gcatagatga gtttatcgta agagagactt tcttgcttgc cgttctcttt
     1141 gagagtgagc aatttttgtt ttgtttggat atccacaact tctgtgcgta tacgtacgtc
     1201 gatattgtag actgcgcgaa aaaaattttc acgctcgcgg ttcaattcat cgagagatga
     1261 aacttcaccc gagaggtggt gggccaaacc ggccatcgca tagctcacac gcgtgttgcg
     1321 ttcgagcaaa ataatttctg ctttttcatt gacctccctc gcacgcgctg cggctgtggg
     1381 acctgcgaga gcggcgccga caataactat tcgcatagcg ggcattttca ataactccag
     1441 cactgaatgt aaatcaaaaa aaacgcgcac aggctcttaa gaacatcgct tgggaaaata
     1501 ttttgctcga tgcagcggat cagaccaagt gctatttctt gcgttccagt ttctgtcctg
     1561 ccggtgtata taaaaagtca acaagcccac ccacgcgata ctgcataaac tcttcttcct
     1621 ctaatgtaaa tacagacgtc gattcgggcg catcactctc accttgccgt tgaatcgcta
     1681 cttcaaataa aacactgggc ttcgttaagc gatagaaagc ggtgcgctct ctgggtgcca
     1741 aaatgatctg gggctgcctg atgggcagct tattcccttc atttgtaaag ctacgcacag
     1801 tattccatac ccatgcacgg taagtcactt cggtatcgta cactgcttct tgtacatagt
     1861 ttgtaacttc gcgctcttta gtgaccattt tttcttctgt cacaggtacg ttttgcttct
     1921 ttgttacgtt acgctccata tagacgggca ctttccggca gcgcgtgatc ttacgggctg
     1981 cacctgaccc cactgactcg tatgtcgtgt tgcactcttc gcgctcgccc gattgtacac
     2041 gttcggttgt tgtaacctgt tgttctcgtg tcgttgcgac ctctattttt tcatcatatt
     2101 tttcggtgcg cgtgccgctt gcaacatcgc gatagttacg cactttacgc atttttttga
     2161 ttatctgtgc accacgcgga acgttgtcct tccacgccgt aaaagtctct atactttttt
     2221 gttcaagaga aactatgatt ttccattttt tgtcgatgac cttgtacacg acaacttgcg
     2281 ttctaaaata tccagttaca cacgaaaccg ttttatagcc gcaccacaga acgcctgttg
     2341 caagaacgat gaataatcca cgtnnnnnnn nnnaaggcag ttttttcttc ttctcttgag
     2401 aatttttact cgacaaattt cttttcggcg tcttgggttt gtcgcctacg cgttgaactg
     2461 gctttccaga agcgtcgctc ttggctccgc aagacgggca aaacacgtcg tggatattgt
     2521 ttgcactcga acagaaatta caaatccaat ctgcacctgt agaaagtcgt tctctggtgt
     2581 tgtcgtcacg gataatctct tcggcatctc tcttacgata gaattggaca tttgcgtcgc
     2641 gtgccttgcc gcacgctggg caaaaccggt gatcaccacg aatacctttt ttcccgcaag
     2701 caagacaatc ccaatactca taaacgtatt ctatcgtaac acgctcattc ataagctatc
     2761 atccgaatgc tcggtatttt cctgcaccat gctacggcta atcgaaatag gaattttact
     2821 tttcaatcaa atgcaaaaac ttctgacact cccctttagt aacaccgaca tgataatccg
     2881 ttttactgta aactttagtt ttacgcactt taccattatc aaagacaatt tcttctgtga
     2941 tgccaaagcc atcatcgaaa cacagtatct tgtcaaagga taacgagtta cctttccata
     3001 gcaaatgacg cggcaaatac gaatcgttca ccaagatcgc agccttgttc gcgaggtcta
     3061 tgatttgaat gcctccactg acatctgtgc cttgatcgag ggccaagtaa cgcaaatttt
     3121 cagagaattt aaaatcggca gtctctatat ttgttttcac tatttgttct ttcttcgttt
     3181 ttgtattaaa aagaacgatt atcaatccct cttcgtacgg cgcattgggg ggatatattt
     3241 tccatgccag atttccacca gagagtttct ggaattcttc actcataaat ccaccaaaag
     3301 accagccttt tttaccttcg ctttcaatct gaaaccatga tgccgtgatt ccatcgatgg
     3361 tcatctctgt agtcgattta gaaagaatac gcacgatggt gccacgcttt agggtaaata
     3421 aagattttgc tttcagatcc gtgctttcac gcactcgcag aacatcggcg gtcacaaccg
     3481 cctcctcagc ttccgcatga acggcggcgg tagtcaggag aaaacaggct aaaaggattt
     3541 tatggtagcg catagaatca ttcgtgcgtg gattattgaa tgcgtaaagg cttttttgtt
     3601 aaattttccc ggttttgacc actccagcac tcgtagggat agttaccccc taatgcgaca
     3661 taatcccgaa aatcgggata gttacccccc aatgtgacat aatccaagaa cgaggcaagt
     3721 gaaatagtta tacaccgtta cggtgaggat atttatatac ccagatttag cgtagaaatt
     3781 tgttgaaata aaatagccct tatttttcag tggttttata atcaaactaa aaaagaaagg
     3841 gctgcctaaa atgttgggcg tgttaatcgc ccgccctgtg tagtcgagga gcatattgcc
     3901 gccgcagcta cacgagggtc atcggagcgc atcgagaccc cagcgctcat ataacgcgcc
     3961 gcgttgcacc aaacaatcgc atcgcgccaa gaaatcgtcg ttacggggtg ctggtcggtt
     4021 ttgccaacgc catcgttgcc ctgaacgcct gcgttaataa tcgtgtagcc gttgctcgct
     4081 gcccacgtct tgactgccac ccagtcgccg tattgcactt cgtactgcac catcaaaaaa
     4141 gtcgttatcg aagggatcgt cgtcaggggt gtggcgacac ccgaccagcc catcaaaaaa
     4201 cctgaggtaa ttcttgattt tttcatttct atcaacaagc ggcaatgtgc gacacatgaa
     4261 ccgtaaagcg aacatttctg atcagtcttg atgcgtacaa cttcaaagcc gcttttcagt
     4321 agcttgtgcc gcatcaccgg ttatcagttc aaaagacatg gccttgccgt cgttcacttc
     4381 aaccaccatt agcgctcgat tgtaatgctg catgagccat cggttcgagg tacgtttgag
     4441 tattttccaa gcgccgaaat catcgtgact ttcaagccgt tcgacaaagg ccgcttccca
     4501 atcacgccga atttttctag tttacagtaa atgcagtact ttgcatggtg cgcaccgcat
     4561 gaaaacacaa ttcaaatttc tcgtctgcat ggcattgatg gccggggggc gtccaaccca
     4621 tacagtgcga aacctgggag ggcggctgca aaaagaacgg acaggggtac ttgcgcattc
     4681 tggacgttca gaaactgaca catgacgcac acaaacaaac caagcagtcg aatactttgc
     4741 ctttttcttt tgaagaagag ggatggcaga acaaggcgct tcaatatatc acaaggcaga
     4801 tggacattat tcaggccgat aagccccggt ttgaggcata cttcctcggt aaatacgcgc
     4861 aagatccttt ctaccactcg agttctcgta aacagattcc gcgccaatag ccgcccgact
     4921 cttacgctca atgcttacat tggcaaatct gcgtcagctc gtgaaagtta atgctactgc
     4981 cacttcagac gaaagtgggc gtcgacgctc tcaaacgtga aatccttgtc ttgctgggct
     5041 gcctcatgga gcgcgggcgt gacaagaatt tcatacgcct cggctgtatc ttcgccgagc
     5101 tttgaggcac tgttgacctc atggccgaag acgtcgacgt ctccgatgcg cagcatcttg
     5161 ccataaccta agccgacgca aagtagcact ttttcttcgt cgagtttatc tttattgtac
     5221 gcgagcagcg tgcgctgcat cgagaccgag gcctgtaacg ctttggtcac attgcgaaaa
     5281 atcacgagta agctatcgcc ctcggctttg agtagaatgc cgtcgtggtc ttcgatcacc
     5341 ggaatcaaga ggcgctccga ctcgaaaatc gtctgtaaga aatggatgat accgaacttg
     5401 gcgacgttgc gcgaaaagcc cgaaagatcg gtgaacatca ctgcccacga ttcgccgaag
     5461 agatcccaga tgcgcgcgtc gattttttgc ttgtcggctc caggtttgag tcgatcttct
     5521 atcaattttt cgagtcggtc ttcgctcgcg tttgcgccga tcattctacg gtatgccata
     5581 gggtatcgaa taaaattttg cgcagccttg caagcctatt cttacaaaat caaactttgt
     5641 aattgcaaag cctgagttat cgaattagtc atcgaatgag atacggtaac cggcgcccgc
     5701 gagaaccgtg aaatagtagc cagacaagtc atcgccacct aatccgccgt ccgtgaattg
     5761 taccttgtgc gccctgacgt cgatttcggc aaagccgttg cgcgacgctg aaaaatactg
     5821 ccgaaatacc aacccgccgt taatattgaa ggagtcgctg aaggaagggc gcaggcctga
     5881 gtaacgaggc acagaacggt ggctaatgag atcgtatcca aaggcacctg ccagcatcag
     5941 cgaagcatcg ccgatatgca gaaaatccca gcggatatct gcggcgatat atacaccaaa
     6001 gaagttgttt gttgcggtga gtactttggt attgaggtca tagtagcgat actcttcagc
     6061 gctgttgcca aaacggaaat ccatagccat tccaacatag aagcgtccaa cacctatagc
     6121 agactgaaat cccattgagg gatgcgc
//
LOCUS       DAOMPD010000109         6094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5507_length_6094_cov_2.738864, whole genome shotgun sequence.
ACCESSION   DAOMPD010000109 DAOMPD010000000
VERSION     DAOMPD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_6094_cov_2.738864"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2334)
                     /locus_tag="PLY93_05115"
     CDS             complement(<1..2334)
                     /locus_tag="PLY93_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRP68892.1"
                     /translation="METDLRIGYFTAQPSSIDPFHGFDPDSYTAITACHDSLTCIGVD
                     GELTPGLAKEWRRIDPVTMEFTLREGVLFHNGEKLNSDAVVATIAAQRDPANHSPTGQ
                     GILGSIQKAEKVDEYRFRLITEFPDAMLLWRLAMFSDIVPPEALARHGVHGFKDHPIG
                     TGPFMFHSMEHGKYIRYVRNPNYWRGAPKIESFRFVIMPAPNWVEALSENKLDLVYGL
                     HSVQAPLLEKNKDIQLTSRLVALSHWFLLSQNGPLADVRVRQAMNYAVDNYMLAKIQG
                     QDKSIPQRCVGTAGQFGFNEHTPIFPFNPDKAKELLAEAGYASGFTLKGMVADHSASL
                     VQMIAAYLADVKIQLEYEVVPRTVWMDRVPNARMSGQGRFAGDFAVAPVDNPIMYSGF
                     HHYIFLSSMGPFSLLANAEYDKRFMDAMSSIDQGEQKLKELDQYAHDEALLLFTLQTS
                     VLVAARKGVSLQITQSGHFGNVGWLSLEDTRPEEKRPEWQYDYLETTKPIAAEDFRKV
                     LEATQHSSDMFFFSSQNLNDPRLEKIVTNLNYQQQIKLMQDKMRFEQIVQYLNKTRDM
                     EGILNASRFSGIANYNMNGDMVLCNPAFVELFGEETRKKPLRSIFPEARDWDKFTATL
                     HSEGGFFGSLNFLRTNGDILEAQAACSMRKGSAGENIGYLMIIRDESEERKLRVDLER
                     SYRELEDKVVQRTSELSKTLDEVQRLKQIQDGDYFLTTLLLQPLGLNQVESEKFSVEV
                     FSRQKKKFTFKKKTYEIGGDLNIAYTVALREKKYTFIL"
     gene            complement(2382..2825)
                     /locus_tag="PLY93_05120"
     CDS             complement(2382..2825)
                     /locus_tag="PLY93_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRP68893.1"
                     /translation="MQPRSCSLLFVRYIPLFFVFLWVACNRPQHRDGDFVFRDWYVVK
                     PRQVGLTSVAYGKIVNSGGTPRVLESVSFACADRSTLHETREENGMVRMAALSNVTLK
                     PHETISFEPAGKHVMLEKMHETSGDLCAAVFQFKTESVRFSIPVR"
     gene            2891..3265
                     /locus_tag="PLY93_05125"
     CDS             2891..3265
                     /locus_tag="PLY93_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68894.1"
                     /translation="MSHGEQENDGKRIAGDDEWSRVGEYLDRLAFENDRLEQELKIKK
                     ERRAAREAESFVFQKTVMGNSTQFNEAHETTLYEARTIATTTDSMHVSYDALRGDLKG
                     LIADLNDFRSSCAGLESRLGNS"
     gene            complement(3262..3879)
                     /locus_tag="PLY93_05130"
     CDS             complement(3262..3879)
                     /locus_tag="PLY93_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68895.1"
                     /translation="MNKKFTEEELNHYYKLLDEPLTPYDCGKLCAPQNGGTPFCCIVE
                     NAVPMLYREEFKCLSKRTKLWRRWSPSHPQDKQQKAEEETEILIFCECKGIAHCERQN
                     RSVACRTFPLEPYFNKEGEFVGLVFMREFQGKCPLTARLKDIKQSVIDNHYEFWRLIM
                     EKKSDEYNLYKNSSRGRRISTTKRGVELPILYPSHLKKAMRSRSA"
     gene            complement(3884..4978)
                     /locus_tag="PLY93_05135"
     CDS             complement(3884..4978)
                     /locus_tag="PLY93_05135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRP68896.1"
                     /translation="MSLGIKDIIRHNDLPNHEPCRSLVDELRRLHMAAITQNLKLLSY
                     FELGKSLHSSTSLSGILEDVLIQIRLITKAKKAYVVLHQATKDQTPIVFTTNRANRIR
                     EFEVSPERANRFLTITEGQIPVVRSLGNMRQLWYGRAQILDSEARDAYLTLVCPIIVR
                     KKRLGDFILIGNRFFDIFAQNEIYLFFGFAEQLGHAILNSRYYQWSFKDSLTGLYVRR
                     QLDEKFAQLVEEYDLKKRKFSLIMIDFDFFKQVNDEFGHVEGDQVLKDFSAFLLKQLR
                     ENDIAIRYGGEEFAVLLPDATATLAQKVAKRIAANLMEFTFSTKKQHTISQGVAEYRG
                     ERELDRFIARCDKALYQAKTSGRNTIVMAD"
     gene            complement(4989..>6094)
                     /gene="thrS"
                     /locus_tag="PLY93_05140"
     CDS             complement(4989..>6094)
                     /gene="thrS"
                     /locus_tag="PLY93_05140"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRP68897.1"
                     /translation="YTSEHLPHYTESMFPPMQMDNENYYIKPMNCPFHHTIFGHRPRS
                     YRELPLRLAEYGACHRYEDSGALMGLMRVRAMQMNDAHIYCTEEQAVEEFKAVIRLHE
                     YYYKILDIKDYHMVLSLRNPASKKYHGEDAMWTKAEALTRQAMEESGVEFIVENDGAA
                     FYGPKMDFQIKSSIGREFTSSTCQLDLFMPMKFNLEYVDKDGTLKRPVCIHRSPLGTH
                     ERFVGFLIEHFAGAFPTWLSPEQVRILPVNEVHESYAKNLADHLRKEKIRVNVELPEE
                     SLGKRIRSAEKMKVPYMLVVGDKEVQANIINARNYFTGEQKEFSQDEFLKMVQEEIRQ
                     KKITEMVKPYATSGAQPETARAVAPASLSNGKG"
BASE COUNT         1520 a         1433 c         1487 g         1654 t
ORIGIN      
        1 gaggatgaat gtatattttt tctcacgcaa cgccacagtg taagcgatgt tcaaatcgcc
       61 gccgatttcg tatgtttttt tcttaaaagt aaatttcttt ttttggcgtg agaatacttc
      121 gacagaaaat ttttcggatt cgacttggtt aagtcccaat ggctgtaaaa gcagcgtagt
      181 aagaaaataa tccccgtctt ggatttgttt taagcgttgc acttcatcga gtgttttgct
      241 gagctcagac gtgcgttgta ccactttgtc ttcgagttca cggtagctgc gctctaaatc
      301 gacgcgcagt tttcgctctt ccgattcgtc gcggataatc ataaggtagc cgatgttctc
      361 gcctgcacta cctttgcgca tactgcacgc ggcttgcgct tcgagaatgt cgccgttggt
      421 acgcagaaaa ttaagcgaac cgaaaaagcc accttcgcta tggagagtgg cggtaaattt
      481 atcccaatca cgggcttcgg ggaaaatact gcgcaaaggc tttttacgcg tctcttcgcc
      541 aaaaagttcg acaaacgcag gattacaaag caccatgtcg ccgttcatgt tatagtttgc
      601 aatccccgaa aaacgcgacg cattgaggat gccttccatg tcgcgcgttt tgtttaagta
      661 ttgcacgatt tgctcgaaac gcatcttatc ttgcatgagc ttaatttgtt gttggtagtt
      721 taagttagtg acaatttttt cgagtcgtgg gtcgtttaag ttttgcgagg aaaagaaaaa
      781 catatccgac gaatgctgag ttgcctcaag cactttacga aaatcttctg ccgcaatcgg
      841 ttttgtcgtt tccaaatagt cgtattgcca ttcggggcgt ttttcttcgg ggcgtgtgtc
      901 ttcgagcgaa agccaaccga cgttgccgaa atggccgctc tgtgtaattt gtaaagacac
      961 gccttttcgt gcggcgacca atacactggt ttgtaatgtg aaaagtaaca atgcttcgtc
     1021 gtgggcatat tgatccaact cttttaattt ttgctcacct tgatcgatgc tactcattgc
     1081 atccataaaa cgcttatcgt attcggcgtt agcgagaaga gaaaatggtc ccatcgacga
     1141 taaaaatata taatgatgaa atccgctata cataattgga ttatccaccg gcgcgacggc
     1201 aaaatcaccc gcgaagcggc cttggccgct catgcgcgcg ttggggacgc gatccatcca
     1261 cacggtgcgc ggaacgactt cgtactctaa ctgaattttc acgtcggcta aataggctgc
     1321 aatcatttgg acaaggctgg cagagtggtc ggccaccatg cctttgagcg taaagccgga
     1381 cgcgtagcca gcttcggcca acaactcttt tgctttatcg gggttaaaag gaaagatggg
     1441 cgtatgttcg ttaaaaccaa attgcccagc agtgccaacg caccgttggg gtatcgattt
     1501 atcttggcct tgaattttag cgagcatgta attatcgacg gcatagttca tcgcctgccg
     1561 cacacgcaca tcggccagcg ggccattttg cgacaataaa aaccaatggc tgagtgcaac
     1621 aaggcgtgag gtgagttgga tgtctttatt tttttcaaga agcggcgctt gtacagaatg
     1681 caaaccgtac acgagatcga gtttgttttc tgaaagtgct tcaacccagt tgggtgcagg
     1741 catgatgaca aaacgaaacg attctatttt cggtgcgcct cgccagtagt tgggatttct
     1801 cacgtaacgg atatatttac cgtgctccat ggagtggaac ataaaagggc ctgtaccaat
     1861 gggatggtct ttgaaaccgt gcacaccgtg gcgggcgagc gcttcgggag gtacaatgtc
     1921 ggaaaacatc gctaaacgcc acaacagcat tgcatcggga aactcggtga tgagacggaa
     1981 acggtattca tccacctttt cagccttctg gattgacccc aggatacctt gaccagtggg
     2041 cgagtgattt gccggatcgc gctgtgcagc gatagtggcg acaaccgcat ctgaattgag
     2101 cttttcgccg ttgtgaaaaa gtacgccttc gcgcagtgta aactccattg ttacggggtc
     2161 tatccgccgc cactctttcg caagccctgg cgtgagttcg ccatcgacgc cgatgcaagt
     2221 taaactgtcg tgacatgcag tgatcgcggt gtacgaatca gggtcaaaac cgtggaaggg
     2281 gtcaatcgat gaaggttgag cggtgaaata gccaatacgc aaatctgttt ccattcacca
     2341 acaatatggt attattgacg cgcggcaagt gatttttgat tttatcgcac tggaattgaa
     2401 aaccgcacac tttctgtttt aaattgaaat accgcagcac acagatcgcc gctggtctcg
     2461 tgcatttttt caagcatgac gtgcttaccc gccggctcaa aagaaattgt ttcgtgtggt
     2521 ttaagggtga cgttagacaa cgccgccatg cgcaccatgc cgttttcttc gcgggtttcg
     2581 tgtagcgtac ttctatcggc gcaagcaaac gacacgcttt ccaaaacgcg cggcgtgccg
     2641 ccagagttta caatttttcc atacgccacc gatgtcaatc ccacttgtct gggttttacg
     2701 acataccaat cccgaaaaac aaaatcgccg tcgcggtgtt ggggccgatt gcacgccacc
     2761 caaagaaata caaagaagag tgggatatac cgcacaaata aaagagagca tgaccgcggc
     2821 tgcatgatat acgcttagaa tatgagactt gctttatcaa gctttttggc gagtacgtcg
     2881 aaaatatact atgtctcatg gtgagcaaga aaacgacggc aagcgtatcg caggcgacga
     2941 cgagtggtcg cgcgtgggcg aatacttaga ccgtttagcg ttcgagaacg accgcttgga
     3001 acaagaactt aaaataaaga aggaacgccg cgctgcgcgt gaagcagaaa gctttgtatt
     3061 tcaaaaaacc gtaatgggca actcaactca attcaacgaa gcgcacgaga ccacgcttta
     3121 cgaggcacgc acgattgcca ccaccaccga cagcatgcac gtttcttacg atgcactacg
     3181 cggcgactta aaaggtctta tagctgattt gaacgacttt cgttcgagtt gcgcgggatt
     3241 agagtcgcgg ctgggcaata gttaagcgct tcttgagcgc atcgcttttt tgaggtgcga
     3301 cgggtataaa atcggtagtt caacgccgcg cttcgttgta gagatacgcc tgccacgcga
     3361 actatttttg tagaggttgt actcgtccga ctttttttcc ataatgagcc gccagaattc
     3421 ataatggtta tcaatgaccg attgcttgat gtctttaagg cgcgcagtaa gtgggcattt
     3481 cccttggaac tcgcgcataa aaacgagtcc cacaaactcg ccttctttat tgaaatacgg
     3541 ctcaagcgga aatgtgcggc acgccaccga acgattttgc cgctcgcagt gcgcgatgcc
     3601 tttgcattca caaaaaatta aaatttcagt ttcttcttcc gctttttgtt gtttatcttg
     3661 cgggtgcgag ggactccaac gccgccagag ttttgtccgt ttagataaac acttgaactc
     3721 ttcgcggtag agcatcggta ctgcattttc gacaatgcag caaaaaggag ttccgccgtt
     3781 ttgtggggcg cacagcttac cgcaatcgta aggcgttaaa ggttcgtcga gtagtttgta
     3841 ataatgattg agttcttctt cagtaaattt tttattcata acgttagtct gccataacga
     3901 tagtgttgcg gccactggtc tttgcttggt agagagcttt gtcgcagcgt gcgataaaac
     3961 ggtctaactc gcgttcgcct cgatactcgg ccactccttg cgaaatggta tgttgttttt
     4021 ttgtagagaa cgtaaattcc attaaattag cggcaatgcg tttagcaact ttttgtgcga
     4081 gcgtggcggt ggcgtcgggt aaaaggacgg caaactcttc accgccatag cggatggcga
     4141 tgtcgttctc gcgcaattgt ttcaaaagaa atgcagagaa atctttcaag acttggtcgc
     4201 cctcgacatg gccaaactcg tcgttcactt gtttgaaaaa atcaaaatcg atcatgatga
     4261 gactaaattt acgctttttc aaatcgtact cttcgacaag ttgcgcaaat ttttcatcga
     4321 gctgacgacg cacataaagg ccggtgagcg aatctttgaa cgaccattgg taatagcggc
     4381 tgttcaaaat ggcatgcccc aactgttctg caaacccaaa aaaaagataa atttcgtttt
     4441 gagcaaaaat atcgaaaaaa cgattgccaa ttaaaataaa atcgcctagt cgtttcttac
     4501 gcacaatgat gggacacacg agcgttaggt aagcgtcgcg cgcttcggag tccaaaattt
     4561 gcgcacggcc ataccaaagt tgccgcatat ttcccagcga acgcacgacg ggtatttgcc
     4621 cttcggtaat ggtcaaaaag cggtttgcgc gttcgggaga aacttcaaac tcgcggatgc
     4681 ggtttgcacg gtttgtggta aacactattg gtgtttggtc ttttgttgct tggtgcaaaa
     4741 caacgtacgc ttttttagct ttggtgataa ggcggatttg aataagcacg tcttctaaaa
     4801 tgccactcag gcttgtcgac gaatgcagcg attttcccaa ctcaaaatag ctgaggagct
     4861 tcaagttttg cgtaatcgcc gccatgtgca agcgccgtag ctcgtcaaca agactgcggc
     4921 aaggctcgtg gttgggtaaa tcgttgtggc gaataatgtc tttgatgcct agagacaatt
     4981 catcctctct atccctttcc attactaagg gatgcaggcg cgaccgctct tgcggtctcc
     5041 ggttgtgccc cggaggtggc gtagggtttc accatttcag taatcttctt ctgtcggatt
     5101 tcttcttgaa ccattttcaa aaattcgtct tgcgaaaatt ctttctgctc cccggtaaaa
     5161 taattgcgcg cgttaatgat gttcgcttgt acttctttgt cgcccacaac gagcatatac
     5221 ggcactttca ttttttccgc actgcggatg cgctttccca aactttcttc ggggagttcg
     5281 acgttcactc gtatcttttc tttgcgtaga tggtctgcca aattttttgc atagctttcg
     5341 tgcacttcgt tcacagggag aatacgcact tgctctgggg aaagccaagt ggggaaagcc
     5401 cccgcaaagt gctcaatgag aaaacccacg aaacgctcgt gcgttcccaa cggcgaacgg
     5461 tggatacaca ccggtcgctt taatgtgccg tccttatcga cgtactctaa attaaatttc
     5521 atcggcataa acagatcgag ctgacaagtg gaggaagtaa actcgcggcc aatcgaactt
     5581 ttgatttgaa aatccatctt gggaccgtag aacgctgcgc catcattctc gacgatgaac
     5641 tcaacccccg actcttccat cgcctgccgc gtgagcgctt cggcttttgt ccacatcgca
     5701 tcttcgccgt ggtatttttt gctcgccggg tttcgaagcg ataacaccat gtgatagtcc
     5761 ttgatgtcta aaattttgta ataatactcg tgcaaacgga tcaccgcttt gaactcttcg
     5821 accgcttgct cttcggtgca atagatatgc gcatcgttca tctgcattgc acgcacgcgc
     5881 atgagcccca taagcgcacc cgagtcttcg tagcggtggc aggcgccata ctcggcgagg
     5941 cgtagcggaa gctcgcggta gctacgtggg cggtgtccaa aaatggtgtg atggaaagga
     6001 cagttcatcg gtttgatata gtagttttcg ttgtccatct gcatcggcgg gaacatgctt
     6061 tcagtgtagt gcggtaaatg ctcgctcgta taaa
//
LOCUS       DAOMPD010000110         6035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5575_length_6035_cov_3.216722, whole genome shotgun sequence.
ACCESSION   DAOMPD010000110 DAOMPD010000000
VERSION     DAOMPD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_6035_cov_3.216722"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLY93_05145"
     CDS             complement(<1..620)
                     /locus_tag="PLY93_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRP68898.1"
                     /translation="MTIEGEFSLDPSTQLFLLTGYSPIVDDNNKVLAYLGLDVSSKDA
                     ARLLDDSIRNSLYVTIAIVFLSLLVSVYVGRVLTGTIVSLDHIVSRFAENDFSVRAKV
                     SSKDEVGRLGYSLNQMATIIEESRARLNALLAAYGRFVPQDYIKLLGKSDIVELKLGD
                     YSQKQMTVLFTDIRGFTSLSEKMSPYENFAFINSFLKRMGPIVRKN"
     assembly_gap    696..742
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<743..949)
                     /locus_tag="PLY93_05150"
     CDS             complement(<743..949)
                     /locus_tag="PLY93_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68899.1"
                     /translation="MRPDEIDATASTKFEDSPVYRKIYDQLNTIRDAYPEMIRYAYTF
                     IKANAEGKALYVVDANVLSSRQRAA"
     assembly_gap    957..988
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<989..1195)
                     /locus_tag="PLY93_05155"
     CDS             complement(<989..1195)
                     /locus_tag="PLY93_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68900.1"
                     /translation="MRQLRLSLRAKIIAAFVVLSTALSGGLSVFNYMHLKDQLFADMR
                     RKLENMAFLGAQSLDKTALAMLVRQ"
     gene            complement(1198..2613)
                     /locus_tag="PLY93_05160"
     CDS             complement(1198..2613)
                     /locus_tag="PLY93_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRP68901.1"
                     /translation="MLLWGILGYLGLTLAVGFLAARRVKSSTDFALAGRNLSFFMASS
                     TVFATWFGSETILGSSAEFAKHGFSGVVEEPFGAALCLILVGLFFARRLYRMRLNNLG
                     EFYRHQFGVPTEIIASVIMAFSYLSWVAAQFLALGIVLNTISGLNLQLCIVLMAVLVT
                     LYTTMGGMWAVSMTDAIQMTVIIIGLVVVFFVVASDFGGVSAVVQMPPPAHWNFLPEA
                     KSASVIGFMSALMILGIGSIPGQDVFQRVMAAKNERIAVFSSIFSGILYFTVALIPLF
                     LGFLALKTLGKNDAPADMQFLIPKLVLLRTNVVIQIVFFGALLSAILSTASGALLAPS
                     VVLSENLLGPLFKPNDEKLLRISRISTVVLAAVSLVMALHYTKITELVSDSSAYGLVA
                     LFVPLVAGMYFKSTNAVAAIASILGGTLVWKLWPEEKTLLTPQLAGFIAALAPYAVLA
                     PFTLVRRFYGKIQPAKNPTGE"
     gene            complement(2994..3311)
                     /locus_tag="PLY93_05165"
     CDS             complement(2994..3311)
                     /locus_tag="PLY93_05165"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRP68902.1"
                     /translation="MLSKEEYDFFLKKYAKGFHVYFQRIAGETSDVIFKAAQYIAFGL
                     FHNSQIKNVDNDTNTLTFRFKSHVDAQSREKTFSSLTMPIQEFMARMLFFLPNRHEKS
                     IRY"
     gene            3769..4203
                     /locus_tag="PLY93_05170"
     CDS             3769..4203
                     /locus_tag="PLY93_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRP68903.1"
                     /translation="MGEKCNFLKSGDIILDPFFGSGTTMVQASELGMHAIGIDISAFN
                     SMIANCKVGRYDLPFVKNELNKITTSLKDFLKNSHTIEFEEELLKKLYEFNNQYFPVP
                     DYKYRLRNGQINEKIYGQEKEKEFFDIYNQLIIKYNIKLRVN"
     gene            4535..4768
                     /gene="vapB"
                     /locus_tag="PLY93_05175"
     CDS             4535..4768
                     /gene="vapB"
                     /locus_tag="PLY93_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRP68904.1"
                     /translation="MNTAKLFKNGESQAVRLPKEYRFKGKEVYITKHSNIVMLIPKSK
                     DGWDLMKSAVSKFSNDIFEGGRQQPKDQDRASL"
     gene            4768..5166
                     /locus_tag="PLY93_05180"
     CDS             4768..5166
                     /locus_tag="PLY93_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRP68905.1"
                     /translation="MYLLDTNICIYIISKKSTKIIDRFKEAKLEGKIGISAITYAELQ
                     YGVSKSIRQTENQVALAQFLIPLITYEFDEKAGIEFGQIKALLEKKDTVIGPYDMLIA
                     GHAISLDATLITSNEGEFRRIEKLKVENWL"
     gene            complement(5383..>6035)
                     /locus_tag="PLY93_05185"
     CDS             complement(5383..>6035)
                     /locus_tag="PLY93_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68906.1"
                     /translation="PTLIKGDDGLNYVLKMKGSGNGTRSLLREYIVNRCCSEVGFLVP
                     KAHIIRIPKEYGWHFGTDEFDDIVQKSYGCNLGIEYIPRTQTVADLRVMLPHDRLFAQ
                     MIAIDLFFRNLDRTYESRNVIVDYNDNHWLIDHGSCEFIHSQSLVTAADFPENHFLHS
                     KFKEYLKLIGPLLSFDFSPILTDIPTEWLAEAGTEIDIVYDSLEFRKKLIRRRYGA"
BASE COUNT         1748 a         1294 c         1398 g         1516 t
ORIGIN      
        1 tggttcttgc gtacaatagg ccccatgcgt ttcaagaagc tattgataaa agcaaaattt
       61 tcgtatgggc tcattttttc cgagagcgag gtaaatccgc gtatgtcggt aaatagaacc
      121 gtcatttgtt tttgcgaata gtcgccgagt tttaactcca caatatcaga tttgcccaag
      181 agcttgatgt agtcttgcgg cacaaaacgg ccgtatgcgg cgagtaacgc attgagtctg
      241 gcgcgcgatt cttcgatgat tgtcgccatt tggttgaggc tataccccag acgcccgact
      301 tcgtctttcg acgaaacctt cgcgcgcacg ctgaaatcgt tttctgcaaa acgcgagacg
      361 atgtgatcga gcgaaacgat tgttccggtg agtacgcggc caacatagac gctcaccagc
      421 aacgataaga agacgattgc gatagtgaca tagagggagt tgcgaattga atcgtcgagc
      481 aggcgcgccg cgtctttaga cgagacgtcg agtcccaaat acgcgagtac tttgttgtta
      541 tcgtcgacaa tgggagagta accggtaagt aaaaataatt gcgtactcgg atccaggcta
      601 aactcgcctt cgatagtcac gcgccgcgtc gccattgcct tcgcggcggc cggatagggt
      661 ttgatatccc atgcggaatt gaaatgcgaa atctcnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nncgcggcgc gctgccgcga cgaaaggacg tttgcatcga
      781 cgacataaag cgccttgcct tcggcatttg cttttatgaa ggtatatgca tagcgaatca
      841 tttcggggta tgcatcgcgt atcgtattga gttgatcgta gattttacgg tatacgggcg
      901 agtcttcaaa ttttgtcgaa gcagtcgcat cgatctcgtc gggccgcatt tgccgcnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gccgcacaag catcgctaac gcggttttat
     1021 cgagcgactg tgcgcccaag aaagccatgt tctcgagttt gcgcctcata tcggcgaaga
     1081 gttggtcttt taggtgcata tagttgaaaa ccgaaagccc tcccgagaga gcggtgctta
     1141 ggacaacaaa tgctgcaatg attttggcgc gcaaactgag tcgaagttgt cgcatagtta
     1201 ctctcctgtg gggttctttg ccggttgtat tttaccgtag aaacgtcgca ctaaagtaaa
     1261 cggcgctaaa acggcataag gcgcgagcgc cgcaatgaac cccgcgagct gcggcgtcaa
     1321 gagggtcttt tcttcgggcc atagcttcca gacgagagtg cctcccaaaa tactcgcgat
     1381 cgcggccacg gcattcgtcg atttgaaata catgcctgcg acgaggggaa caaaaagagc
     1441 gaccaaacca tacgcagacg agtcggagac gagttcggta atttttgtgt agtggagcgc
     1501 catcacgagc gagaccgccg cgagtacgac ggtggatatc cgcgaaatgc gcaaaagctt
     1561 ttcgtcgttc ggtttaaaaa gaggtccgag tagattttcg ctcaaaacga cagacggcgc
     1621 gaggagcgca cccgaggcgg tcgaaagtat cgctgaaagt aacgctccaa aaaaaacgat
     1681 ttgtatgacg acgttcgtgc gcaggagcac cagtttgggg atcaagaatt gcatatcggc
     1741 gggcgcgtcg tttttgccga gagtctttaa cgccaagaag ccgagaaaga gcgggatcaa
     1801 ggcgaccgtg aaatagagta tgcccgagaa gatcgacgag aaaacggcga tgcgctcgtt
     1861 cttagctgcc atgacacgct gaaagacgtc ttggcccgga atggagccga tacccaatat
     1921 catcagtgcg ctcataaaac caatcacgct cgcactcttc gcttcgggta aaaaattcca
     1981 atgcgccggc ggcggcattt gcacgaccgc cgagacgccg ccaaaatcgc tcgccacaac
     2041 gaaaaagacg acgacgagcc cgataataat caccgtcatc tgaatagcgt cggtcatcga
     2101 gaccgcccac atgccgccca tcgtcgtata gagtgtgacg agcactgcca tgaggacgat
     2161 gcacagttgt aagttgagac cgctgatggt attgagcacg ataccgagcg cgagaaactg
     2221 cgccgccacc cacgaaagat atgaaaacgc cataattacc gatgcaataa tttccgtcgg
     2281 tacgccgaat tggtggcggt agaattcgcc caagttattc aaccgcatcc ggtataaacg
     2341 ccgcgcgaaa aataacccga ctaaaataag gcagagcgcg gcgccgaacg gttcttcgac
     2401 gacgccgcta aatccatgtt ttgcaaattc tgccgaagaa cccaagatcg tctccgaacc
     2461 gaaccaggtt gcgaagaccg tcgacgacgc catgaagaaa gaaagattac ggcctgcgag
     2521 cgcgaaatcc gttgagcttt tgacgcgcct tgccgctaaa aaaccgacag cgagcgtgag
     2581 gccgagatag ccaagaatac cccaaagtag cacgcacgac cgagcgtgag aagcgatggc
     2641 accgtcaagc tagacaattg taaataaaaa ggtgaaatta atctgagcta aatcagacac
     2701 ttatatctac aaagatttga taaaattcgt cagagctaac gttaagtata taggaaatta
     2761 ctctccattg gggcttatag aactcataat ctctccggtg taaagcctgt ttctggtggt
     2821 gaaaaaatga ccagttatga ccagttacgg ccttaaaggg ctatattttt tgccgaaaac
     2881 gccatttttc aggctttttt tgtgaatggc gtgtaacatc cccggcgcac tcttaatgtg
     2941 taaaaatggg taaaaaaacg cttagtgagg tttatggcct ttcgggcggt gccctagtag
     3001 cgaatcgatt tttcatgcct attgggcaag aaaaagagca tgcgagccat gaactcctga
     3061 atcggcatcg tcaatgaaga gaatgttttc tcccggctct gcgcgtctac gtggcttttg
     3121 aatcgaaacg ttagcgtgtt cgtgtcgttg tcgacgtttt tgatttggct gttgtggaag
     3181 agaccgaacg cgatgtattg cgccgcctta aatatcacgt cgctggtttc gccggcgatt
     3241 ctctgaaaat aaacgtgaaa tcctttcgcg tacttcttca agaaaaaatc atattcctct
     3301 ttcgatagca tccttcgtcg tcgcccggcc ctcctagccg ggctctgaag acagagattc
     3361 gcctacggct cgtcgcacag gcgcaataat taacctgctg cgcgcgctcg cactccgttg
     3421 gcgcgtgtgc tcggccattc gcgccgcaga tgccctcgtc gcacaaacgg gacgtttgtt
     3481 ttgtgctttg ccgcgaggcg cttcgggcac attttttgac gcaatttagg tttacaaatt
     3541 gaaaacttaa acgatgcgtc actaaacgtc ggcgtaaact ccacgttatc ggaaagacag
     3601 ggtgcagaat gtttgtaaaa aacagataat tatgatatta ttaccctacc ccgaagacgc
     3661 ccgtcgtcct tgacgggcga agaataaata aatggtatgg tataaaatga aaatcatgaa
     3721 tgaagatttt atagatgtga aaactgccag tgaatttgct acagaattat tggggaaaaa
     3781 tgtaacttcc tcaaatctgg agacattata cttgatcctt tttttggtag tggtacaaca
     3841 atggttcaag catcagaact tggaatgcat gcaataggca ttgatatttc tgcgtttaat
     3901 tctatgatag caaattgtaa agttggaaga tatgatttac ctttcgtaaa aaacgaatta
     3961 aataaaataa caacttctct taaagatttt ttaaaaaact cacatacgat agaatttgaa
     4021 gaagaacttt taaaaaaatt atatgagttt aataatcaat attttccagt cccagattat
     4081 aaatatagat taagaaatgg tcaaattaat gaaaagattt atggacaaga aaaagagaaa
     4141 gaatttttcg atatatataa tcagcttatt atcaaataca atatcaaatt acgagtcaat
     4201 taaaggcgac agaatccgct gacacagggg gctaaaagag tggtttttat attctgaaga
     4261 aaagttatta cagaatgata attcgataaa aaaaagaaca atagatgata aaacattaaa
     4321 tagagtctat aataaaaatt catttgatag taaaggaaat tatgattatg atagatataa
     4381 tcaattaata agtggcttat aaaaaagcct acacgcgtcc gtgcgcttcg gcttcaaaat
     4441 gtcgtgtaaa ttccacgttg tctaaaattt gcgaagacca aacttttctg aatatttttt
     4501 tgccgaattc agaatagtat atattttagt atatatgaat acagcaaagc tctttaaaaa
     4561 tggcgagagc caggcagtcc gcctaccgaa agagtatagg tttaagggga aagaggtcta
     4621 tattaccaag cattccaata ttgtgatgct aataccgaaa tctaaagatg gctgggacct
     4681 tatgaaaagc gctgtttcta aattcagtaa tgatattttc gaaggcggcc ggcaacaacc
     4741 gaaagatcaa gatcgagcca gtttataatg tatttgcttg atacgaatat ttgcatctat
     4801 attatctcaa agaaatctac caagataatt gatcgcttta aagaagcaaa gttagagggg
     4861 aaaatcggta tctctgccat aacttacgca gagttacagt acggcgtgtc caaaagcatt
     4921 cgacaaaccg agaaccaggt tgctttggct caattcctta tccctttgat aacttatgaa
     4981 tttgatgaaa aggctggaat cgagtttggc cagataaagg cacttcttga aaagaaagat
     5041 acggtgattg gaccatacga tatgctaatt gctggacatg caatatcact agatgcaaca
     5101 cttattacta gtaatgaagg cgagtttcgc cgaattgaga agctaaaagt tgaaaattgg
     5161 ctttaacgat cgcaaacttc agacaacaaa ttttacgcgc tgcgggcgcg agcactccgt
     5221 tgattcacgt cctcgccctg cgaccgctaa gatcgttgac gcggcacaag cgggacgctt
     5281 gcttttgcgc gccgcccacg atcttatgcc gctcaatgcc tagctattcc ttaaggaaaa
     5341 gcatagattt ttgatttctc ggagcctttt tcgatagtct tgttaggcgc cataacgccg
     5401 gcggataagc ttctttctga attcaagaga gtcataaacg atatcgattt ctgtgcctgc
     5461 ttcggctaac cattctgtcg gtatgtccgt taatataggt gaaaaatcaa aggataagag
     5521 aggaccgatt agtttcagat actctttgaa ttttgaatgg agaaagtggt tttcaggaaa
     5581 gtcagccgca gtgacgaggc tttgtgaatg gatgaactca cagcttccat gatcaatgag
     5641 ccagtgatta tcgttataat ccacaatgac atttctactt tcatatgtcc tatcaagatt
     5701 gcggaaaaag agatcaattg cgatcatttg agcaaataaa cgatcatgcg gtagcatcac
     5761 acgtaagtcg gctactgttt gcgttctagg aatatattca attcccaaat tgcatccata
     5821 gcttttctga acgatatcat caaactcgtc tgttccaaaa tgccagccat attctttcgg
     5881 aatgcgaata atgtgagcct tggggacgag aaagccaact tccgaacaac acctgttaac
     5941 aatgtactcg cgcaatagac ttcgagtccc gtttccagag cccttcattt tcaagacata
     6001 attaagtccg tcatcgccct tgatcaatgt tggaa
//
LOCUS       DAOMPD010000111         6001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5604_length_6001_cov_4.087790, whole genome shotgun sequence.
ACCESSION   DAOMPD010000111 DAOMPD010000000
VERSION     DAOMPD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5604_length_6001_cov_4.087790"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PLY93_05190"
     CDS             <1..1455
                     /locus_tag="PLY93_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68907.1"
                     /translation="NTPQLRRSICQVCPWVRQINNNFSIVDVFEIQFTDTVESTSNQN
                     FTITGFNKVGSIVTGKSVSASVYNGNDNDDDFNNDNSRGNILSYRIPPALGTAMVQPR
                     VAYTTTTSFSMQSRILLQNKAVAPGSNRIFRAQITLPAGVTVANASDVTPRTGTTVAC
                     AGGDGFTAGCSGPGESITLDHTTATPSYLDPNGTSSDEVLVLWSHNLSAEGNLVFGIN
                     FDNNDGNGFAANLEAVPAALRTVELVNEPTIRIEVISKPGDPANPASLINDQTANVIV
                     PETSYHRFRLFMNANSGSKKIHRIRITPPSPFIASSLDSGDLLPAGTTMTVSGSDLLV
                     SFATAELSAATVDLEFRLQENRNFNTPGVFDNSEDYSWPVSVAYFEDLYDNGVYAENN
                     LPGPSFTGKLNSDGVNDALHYFPGNAFGNARNIIPVSGTGSASLNWNVIRLVNPAPRF
                     MSYVDPEEVIRINPGDPDYTWSQSVYFENKLNPI"
     gene            1546..2457
                     /locus_tag="PLY93_05195"
     CDS             1546..2457
                     /locus_tag="PLY93_05195"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP68908.1"
                     /translation="MEGQCPACGSKIDKYDINDYFRCTSCGNLLTHTQQDQIILISDM
                     TTLTKEKIDELLKDKLPDSEIAALDLAMIKHASALPLLVEAYEKFTNSEHEFKNYTLI
                     DETLLGCIENIDDPGAGPFLIEALNSKDDFVFSAVCSAIENLQYEPALETLVKKVKDK
                     NQGMRSSAVSALGGLYNKAAVQTLLECLSDDDYYVRKFALYALDKIGDVNCQSEILKL
                     ATLEINKTTRDYEKLLPVKELAISVLGNLGNREILKSINKMTLDFHTGIQNAATEAVR
                     KIKNRHPHPLSLSNIIFAFLGLFGVAR"
     gene            2454..3380
                     /locus_tag="PLY93_05200"
     CDS             2454..3380
                     /locus_tag="PLY93_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP68909.1"
                     /translation="MIKITGLNCINCGGSLNQEEDSNFLRCDYCGSFMVLNSQDAALE
                     VQDPVLLTNRLAHQVFNGSKKESIRAMYEIRKIKHAKALPVFVENYIKAYEERQDFYS
                     QKDARQLESQIETFSYGIENCDDPAAEDQVLDLLSHEKKIIIRQALANLEKFPRGKAR
                     PEILELLQHDDQFIRIGAMYALLAFRNPEDLNHIISKLHDKSSFVVEMAIEILAQNNV
                     TRCVPELARLAEITYEYSDNDSDDKLKVRSAAIYALGLLGNEDVVDLLIRLYDSNNSE
                     IEEEVIGALTKLMTRVSESGKNKIQDFLNKVS"
     gene            3521..4168
                     /locus_tag="PLY93_05205"
     CDS             3521..4168
                     /locus_tag="PLY93_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP68910.1"
                     /translation="MIHRLSQTFIFIPIIFLSCSLLKTGQGANCLNPTSGCFRADLTA
                     PRITASDPADGTSLNFLDSISVTVSEKLRSGDSPTNWSLTGAGTNNLTITSVSFDETN
                     YVYTLSISGNVRNGLIQVTALANDFAGNSLQNPSFSFTGNVDIGIAYTISTNYITSGT
                     GFNQSTVNFHHDFADDNDNSWKAYLNSSDCSGTQVAAGTGFVASGIAKKYSPLRP"
     gene            complement(4157..5299)
                     /locus_tag="PLY93_05210"
     CDS             complement(4157..5299)
                     /locus_tag="PLY93_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68911.1"
                     /translation="MTTTHQAGFRYRWIGIGDLNAFFGLMLDNVTNLVFLAQILILVF
                     GFPSDLVYGKMFPGTAFGVLVGDLIYTAMAFRLAKRTQNPNVTAMPLGLDSPSTIGIA
                     FAVIGPAFIAAKSSGLDPQAAGLLAWKVGMATLVIIGVVKFILSFFGDFVEKLIPTAG
                     LLGSLAGVGIALLGLLPLEHVFGTPVAGLVALGIIIYALVAKVRLPFSVPSVFLSVVG
                     GTIIYYILASMKLVHPPELSLKFIVAFPVPTLEFMGGFKEALVYLPVAIPFALLTVIG
                     GINVTASARLAGDSYRTSSILLTEAVATLAAGVTGGVAQSTPYIGHPAYKAMGGRAGY
                     TLATGLFIGLVYKTLLPIGAYGTHDLSGVKPVFGGDKMTSYGKLWP"
     gene            5619..>6001
                     /locus_tag="PLY93_05215"
     CDS             5619..>6001
                     /locus_tag="PLY93_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68912.1"
                     /translation="MLKTSISLPKDSVATWSQNHWAIMRMYARFLRVVERKRKFRRGV
                     TRQYNWGNGHCVIVKVRFTPKEYDSLHTVAAGARVSVSYLIYYLIQFWERMSKKSGKF
                     VVGGNYAPKVLEWSKKRVNFEEKLT"
BASE COUNT         1766 a         1303 c         1335 g         1597 t
ORIGIN      
        1 aatacccccc aattgcggcg tagcatttgt caggtatgtc cgtgggttag acagatcaat
       61 aacaactttt cgattgttga tgtgtttgaa attcaattta cagacacggt agaaagcaca
      121 tcaaatcaaa actttacaat caccggcttc aacaaagtcg gcagtatagt cacaggcaaa
      181 agtgtttcag ccagtgttta taacggcaat gataacgatg atgattttaa taatgataat
      241 tcccggggaa atattttatc atatcgtatt ccgccggcat taggcactgc catggttcag
      301 ccaagagtgg cttataccac cacaaccagt ttcagtatgc aatcccgcat tttattgcag
      361 aataaagcgg tagctcccgg ctctaaccgg atttttcggg cgcagatcac cttaccggcc
      421 ggggtgacag tcgcaaatgc ttctgatgtc accccgagaa ccggaaccac cgtggcctgt
      481 gccggcggag atggttttac tgcgggatgc agtggcccgg gggagagcat tactcttgat
      541 catacgacgg caaccccgtc atatcttgac cctaatggca catcttcaga tgaagtgctg
      601 gtattatggt ctcataacct tagtgccgag gggaatttag ttttcgggat caattttgac
      661 aataacgacg ggaatggttt tgcggccaat ctcgaagcag tgccggcggc tttacgcacg
      721 gtagaattgg tgaatgagcc aaccattaga atagaggtaa tttctaaacc gggtgacccg
      781 gcgaatcccg catctttaat caatgaccag acagccaatg tgattgtgcc tgaaacatct
      841 taccaccggt tcaggctgtt tatgaatgcc aattcaggtt ctaaaaaaat acaccgtatt
      901 agaattaccc cgccgtcacc ttttatcgcc tcatcgcttg attccggtga tttattaccc
      961 gcaggaacaa cgatgacggt atcgggcagt gacctgttgg tcagttttgc cacagcggaa
     1021 ctctcagcgg ccacagtaga ccttgaattc cgtttacagg aaaaccgtaa ttttaataca
     1081 ccgggggtat ttgacaactc tgaagattat agctggccgg tcagtgttgc ctattttgaa
     1141 gatctttacg ataacggggt ttatgcggaa aacaatttgc ccggcccgtc atttaccgga
     1201 aagttgaatt ctgacggggt taatgatgcg ttacattatt ttccgggtaa tgcattcggt
     1261 aatgcgagaa atatcatccc ggtttcaggt acagggtctg cctcattaaa ctggaatgtg
     1321 atacgattag tgaatccggc tcccagattt atgtcgtatg ttgatcctga agaggtaata
     1381 cgtattaatc cgggtgatcc tgattatacc tggtctcaat cggtgtattt tgaaaacaag
     1441 ctaaacccta tttaaaaaaa caactggtgg tttttttctg cattaaagat cagaagttaa
     1501 gatttaattg acattgaaaa taaagattaa aaccatacat tcataatgga aggccagtgc
     1561 cccgcctgcg gaagcaaaat agacaaatat gatataaatg attatttccg ttgcacgagt
     1621 tgcggtaacc tgctgactca tactcaacaa gatcagatca ttctcatctc tgacatgacg
     1681 acattaacaa aagaaaaaat agatgagtta ctgaaagata aattgcctga ctcagaaata
     1741 gccgcgcttg atcttgccat gattaaacat gcatcggcat tacccctttt agtagaggct
     1801 tatgagaaat tcaccaactc tgaacatgaa tttaaaaact acacattgat agatgaaacc
     1861 ctgcttgggt gtatagaaaa tatcgatgac cccggggccg gtccgttttt aattgaagcc
     1921 ttaaactcaa aagatgactt cgtgtttagt gcggtctgtt ctgcgattga aaacctgcaa
     1981 tacgaacccg ccttagaaac cctggtaaaa aaagtgaaag ataaaaacca ggggatgcgc
     2041 tcgtccgcag tgagtgcttt aggcgggtta tataataaag cggcggtaca aacattactt
     2101 gagtgtctct ctgatgacga ttattatgta cgcaaatttg ccttgtatgc attagataaa
     2161 attggtgatg taaactgcca gagcgagatt cttaaattag ccactcttga aatcaataaa
     2221 accacccggg attatgaaaa attactacct gtcaaagaac ttgcaatttc agtattaggt
     2281 aatctcggca accgagaaat tcttaaatca ataaataaaa tgaccctcga ttttcatacc
     2341 ggaattcaaa acgccgcaac tgaagctgtt cgaaaaataa agaaccggca tcctcacccg
     2401 ctttcgctgt ctaatattat ttttgcattc ctgggtttat tcggggttgc gcgatgataa
     2461 aaatcacggg tttaaactgt ataaattgcg ggggcagtct aaatcaggaa gaagattcta
     2521 actttttgcg ctgtgactat tgcggcagtt ttatggttct aaattcacaa gatgcggccc
     2581 tggaagtgca agaccccgta ttactcacta accggttggc ccatcaggtt tttaacggct
     2641 caaaaaaaga atccattcgt gcgatgtatg aaattcgaaa aataaagcat gcaaaagcat
     2701 tgccggtttt tgtggaaaat tatatcaaag cttatgaaga aagacaagat ttttactctc
     2761 aaaaagatgc ccggcaatta gaatctcaga ttgaaacatt ttcctatggt atagagaatt
     2821 gtgatgatcc cgccgccgaa gaccaggttc ttgatttgtt atctcatgag aaaaaaataa
     2881 tcatcaggca ggcattggcg aatctcgaaa aatttccccg cgggaaagcc agacccgaaa
     2941 ttcttgaatt actacaacat gatgaccagt ttatacgcat aggggccatg tatgctttat
     3001 tagctttccg taaccctgaa gatttaaatc atattatcag caaactccat gacaaatcaa
     3061 gttttgtggt agaaatggcg attgaaattc tggcacagaa taatgtgacc cgctgtgtgc
     3121 ccgaattggc gcgattggca gaaattactt atgaatattc agataacgac agtgatgata
     3181 aacttaaagt gcgcagtgcc gcaatttatg ccctcggttt actgggtaat gaagacgtcg
     3241 ttgatttact catacggtta tacgatagta ataattcaga gattgaggaa gaagtgatag
     3301 gtgctttaac caaactaatg acccgggtca gcgaaagcgg gaaaaataaa attcaagatt
     3361 ttctgaataa ggtttcataa caattctcgc tgagaatttc tgaaggtttt agaggccaaa
     3421 aagtcctttt tatacagtct cttacttgat ttggtgttac atgttttgtc tcgctctcgc
     3481 ggtttaattc agttatgaaa ttttttaggc tcacaatttt atgattcacc ggctttctca
     3541 aacattcatt tttattccca taatcttttt atcctgttca ttattaaaaa ccgggcaggg
     3601 ggctaattgt cttaacccaa cgtcgggctg ttttcgggcc gacctcactg ccccccgcat
     3661 cactgcgagt gatccggctg atggcacttc tctaaatttt ttagactcca ttagtgtcac
     3721 agtctcagag aaactcaggt cgggagattc acccacaaat tggtcactga caggggccgg
     3781 gactaacaat ttgactatca cctctgtcag ctttgatgag acgaactatg tctacactct
     3841 cagtatctcg ggtaatgtaa gaaacggtct cattcaggtt accgcactgg caaacgattt
     3901 tgcgggaaac tccctgcaga acccgtcgtt cagttttacg ggtaatgttg acatcggtat
     3961 tgcttacact atttcaacca attacatcac ctcaggcacc ggttttaatc agagtaccgt
     4021 caattttcat catgattttg ccgacgacaa cgacaactcc tggaaggcct acctgaattc
     4081 ttctgactgc tccggaaccc aggttgccgc cggcacgggt tttgtcgcct caggcatcgc
     4141 gaaaaaatat agtcctttaa ggccataatt tcccataact ggtcattttg tcacctccaa
     4201 aaacaggctt tacaccagag agatcatgag ttccgtaggc cccaatgggg agcaatgtct
     4261 tatatactaa accgataaaa agtcccgttg ctaacgtgta accggcacga ccacccatcg
     4321 ccttgtacgc gggatgaccg atgtagggtg tcgattgcgc cacgccgccc gtgacccctg
     4381 ccgcgagcgt cgccaccgct tcggtcaaaa gaatgctgct cgtgcggtac gaatcgcccg
     4441 ccaagcgagc gctcgccgtt acgttgatac cgccgatgac ggtgaggagc gcaaatggaa
     4501 tcgccaccgg taagtacacg agcgcttctt tgaaaccacc catgaactcg agcgtcggca
     4561 ctggaaaagc cacaataaat ttgagcgata gctcgggagg atgtaccaac ttcatagaag
     4621 ctaatatgta gtagataatc gtcccaccca caactgacaa aaacacactg ggtacgctaa
     4681 acggcagccg cacttttgcc accagtgcat aaataataat accaagagcc accaaccccg
     4741 cgaccggtgt gccgaaaaca tgctctaatg gaaggagacc taagagtgca attcccacgc
     4801 cggcgagcga gcctaaaagt ccagcagtgg gtatcaactt ttcaacaaaa tcaccaaaga
     4861 aagaaagaat aaatttcacc acgccgataa tcaccagcgt tgccatacct actttccacg
     4921 cgagaagtcc ggcagcttga ggatcaagcc ctgaactttt agcggcgatg aacgcagggc
     4981 caatgactgc aaacgcgatg ccaatcgtcg aaggtgaatc gagccccaaa ggcatcgcgg
     5041 tgacgttggg gttttgggtg cgctttgcga gtcgaaacgc cattgcggta tagatgagat
     5101 cgcccacgag cacgccaaag gcagtacccg gaaacatttt tccatacact aagtcggagg
     5161 gaaatccaaa gacaagaatt aaaatttgcg caaggaaaac aaggttcgtg acgttgtcga
     5221 gcatgagacc aaaaaacgca ttcaggtcgc cgatgccaat ccaacgataa cgaaatcctg
     5281 cttggtgggt tgttgtcatg gataacaaat tagagcttta ataaaagggg tcaactcaaa
     5341 aacttccgct aacggatttg tgcaactatg cgattatatc tctttagata taaaatcatc
     5401 ttttagcatc taatttatga gaggtaattt tttcaaattt agaattatgt cacatcaggg
     5461 gataactatc tccactgcgt tatgtcgcat tagggggtaa ctatcccaag ctgcgattta
     5521 gcgcaaaaaa tcacactttt tttagaaaat ttgtactttt tttctcaaaa gtgagaaaat
     5581 ttgagcgttt tctggtattt aatatataga ggacaaaaat gttaaagaca tcgattagct
     5641 tacccaaaga ttccgtcgcg acttggagcc aaaaccactg ggcaatcatg cggatgtatg
     5701 cacggttttt gagggtcgtc gagcgtaaaa gaaaatttcg acgtggtgtg acgcggcagt
     5761 ataactgggg gaacgggcat tgtgtcatcg tgaaggtgcg ttttacgccg aaggagtacg
     5821 attcactgca taccgtggcg gcgggggcgc gcgtgagtgt atcgtatctc atttattacc
     5881 tcattcaatt ttgggaaaga atgtccaaaa aaagtggcaa attcgttgtt gggggtaact
     5941 atgcgcccaa ggtgctggag tggtcgaaaa agagggtaaa ttttgaggaa aaactcactt
     6001 t
//
LOCUS       DAOMPD010000112         5960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5639_length_5960_cov_2.591533, whole genome shotgun sequence.
ACCESSION   DAOMPD010000112 DAOMPD010000000
VERSION     DAOMPD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5639_length_5960_cov_2.591533"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLY93_05220"
     CDS             complement(<1..696)
                     /locus_tag="PLY93_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP68913.1"
                     /translation="MSVSLSLSNDRYNPQAAMRSMPNFTQAIAAYSSDATWNIDTNRL
                     PAALKSYRQKCRAFAQRYIQPHALSLDTQKDKTVQEAILAAAGKQGLLSDMLPWPLGS
                     ASIATMLEPLHWVQSIKMEEFCAACGGLGLMLGAHGLGMAPLVLSGDMRALVKFVLPM
                     NRENSAGKMHVAAFAITEPGAGSDVEETEGARTYTPGTTAKKVSGGWLLNGHKVFISG
                     GDIARSITVFAAIE"
     gene            complement(693..1820)
                     /locus_tag="PLY93_05225"
     CDS             complement(693..1820)
                     /locus_tag="PLY93_05225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP68914.1"
                     /translation="MDTNAVNEYLAHVADFRKRYLESAFERSEVICAPNDFLSWREKC
                     VHLGLAVPLDTSVFNEKKSATIFAGGAALQFYLLSLKEIAEANVSLALATHALLLNNA
                     LLQFFSSTKIESDTLYIHPHGRTNLARLALAHFLSGKTTVDDPSFLSEHFDLSQPLWL
                     YATKAPQHILAPAYNKKTHALSWGIYKADLYRDINTLSTHGWDELSYLEVQVGDLISP
                     LDNIDTHSAQTLLANLLAMQALVQVAVGAGALERALKKMQRYISERKQGGVLIQEHAA
                     VRRMVGQVVHTIETVKLSLNGAVYAHGDFITIQSALSIKANLLPQMQSAANAALQAFG
                     GYGFMRDYGMEKIMRDINSLALYAGTPSAIELFLSAYRGGI"
     gene            complement(2301..3821)
                     /locus_tag="PLY93_05230"
     CDS             complement(2301..3821)
                     /locus_tag="PLY93_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRP68915.1"
                     /translation="MRPLLNREQIRFYDKLAIEKYRIPSLILMENAGRGAADKIRSFF
                     LSGSKKPNIVILCGTGNNGGDGFVVARHLAEEANVKVLLTGDATNFSKDAEANYTLLQ
                     HILTVTIHRLNLGVDGTVELLLKEADIVIDALFGTGLTRAIEGVQKNIIRDANNTNAL
                     RVALDIPSGIDADTGAVLGVAFKAHHTLTFAYPKCGLYTPTGKDYAGTIHTLALGVPN
                     GMLLQETGATAHLLEEGDIPLFHTPRSATTYKHRAGDVLIVAGSSGKTGAAQLASLAA
                     LRTGAGLSTIAAWGETWDEYANRAAEIMLLSIERTKIKESLESALSKRNSIVVGPGIG
                     LDADAKKVVLYLLEHSSAPLVLDADALTLVAENVDTLKKSPAQKILTPHSGELARLLK
                     TTATAIESNRFAAVKEAARLTNATVVLKGAHTLIADPHTCRVNPFANPLLATAGSGDV
                     LAGMCGALIATMPPLEAASAAVYSHASTAERWYKKTNRTRGMIASDIIDALVGDYH"
     gene            complement(3831..4484)
                     /locus_tag="PLY93_05235"
     CDS             complement(3831..4484)
                     /locus_tag="PLY93_05235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP68916.1"
                     /translation="MKDNNARRKRGFNFIAPFYDFLASFFFVGKLFKAQTYFIPQLKK
                     ARRALVLGGGTGKILTTLLKNNTAEEYHYIDIAEAMVERAQKRTRTFAHTHCIRFTCA
                     ALENAPLEKYDLIVTPFILDCFDDVSLEKILPRLSTALSKNGEWLFVDFNIPTGKRRV
                     LAHAIVKFLYFCFNLLCALDVKKLPLFEKLFAQVGFQRIHEKYFLYKLVAARVYHKT"
     gene            complement(4481..5338)
                     /locus_tag="PLY93_05240"
     CDS             complement(4481..5338)
                     /locus_tag="PLY93_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRP68917.1"
                     /translation="MLGTALQRFFFYTEVLSLDIAIGVLGSGAFAKTVLHAPMKGTWW
                     FLLCAAVWVVYTADHLMDARKVGKVAVNVRHKFHVEHFRIIFTTMCIVSLLCAILAWV
                     YLREIIFMGGIVVGIFALFHLILAYWGKVRFGKEISVAVIYAMGVWFAPFLNHRGAID
                     TLPILFFSFFLLAALLNLFMNSVIEHALDHKENLIFGVRPRFVQNIRKGVIAISFLSV
                     LFLCALAIYFFFQTSPIDAACAFYVLLLCATPGAILYFDRFFLVRQRYRIAAEWLFAF
                     GLLLLFFNA"
     gene            complement(5348..5827)
                     /locus_tag="PLY93_05245"
     CDS             complement(5348..5827)
                     /locus_tag="PLY93_05245"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRP68918.1"
                     /translation="MQSVLFVCLGNICRSPLAQGIFEKQTADLYLSAFYDADSAGTSA
                     WHQGEKPHSGSLAVARRHGVSIEKQKSRPVHAKDAELFDYILAMDTDNRNNLLTEFHF
                     PEEKVFLMRDFHANGKKGLSVPDPWGHGDDAFENVYQILDESMRGFIAFLQKNKPQP"
BASE COUNT         1546 a         1423 c         1516 g         1475 t
ORIGIN      
        1 ttcgatagcc gcaaagacgg tgatcgagcg tgcgatgtcg ccgccggaga taaaaacttt
       61 gtgtccgttc aagagccatc cgccagatac tttttttgcg gtggtaccgg gggtatatgt
      121 gcgcgcgccc tcggtttctt caacgtccga gcctgcacca ggttcggtga tggcgaaggc
      181 ggcgacatgc atttttcctg cgctattctc gcggttcata ggcagcacaa attttacaag
      241 cgcacgcatg tcgcccgaaa gaaccaatgg agccattccc aacccatgcg cgccaagcat
      301 aaggccgagc ccaccacacg cggcacaaaa ttcttccatt ttaatggatt gaacccagtg
      361 cagcggttca agcattgttg ctatagatgc cgagcccaat ggccacggca gcatgtccga
      421 aagtagcccc tgtttgcctg ctgcggcgag gattgcttct tgtactgttt tatctttttg
      481 tgtgtcgaga gatagcgcgt gtggttggat gtatctttgt gcaaacgcac ggcatttttg
      541 acgatagctt ttcaacgctg ccggcaaacg atttgtgtcg atattccatg tggcatcgct
      601 tgagtaagca gcaatcgcct gtgtgaaatt tggcatcgaa cgcatcgcgg cttgtgggtt
      661 gtaacgatcg ttactcaacg ataaagatac gctcatattc cacctcggta tgcgcttaaa
      721 aaaagctcaa ttgcactcgg cgtgcctgca tacaaagcaa ggctgttgat gtcgcgcata
      781 attttttcca taccatagtc gcgcataaaa ccatagcccc caaacgcttg cagcgcagca
      841 tttgcggcgc tttgcatttg aggcaaaagg tttgctttga tagatagcgc gctttggatg
      901 gtaataaaat caccgtgcgc atacacggca ccgttgagcg agagcttcac cgtttcgata
      961 gtgtgcacaa cttgccccac catacggcgc accgctgcgt gctcttgaat gagtactccg
     1021 ccttgtttgc gctcgctaat gtaccgttgc atttttttaa gtgctcgctc caacgcaccc
     1081 gcaccaacag caacttgcac aagcgcctgc atcgctaaca aattggcaag gagtgtttgg
     1141 gcgctgtgcg tatcgatgtt gtcgagagga gaaattaaat cccccacttg gacttcaaga
     1201 tacgaaagtt cgtcccaccc gtgcgtactg agcgtattta tgtcgcggta taaatcagcc
     1261 ttgtagatgc cccacgacaa cgcgtgcgtt tttttattgt atgcgggcgc aagaatatgc
     1321 tgcggcgctt tagtcgcata aagccaaagc ggttgcgaca aatcgaaatg ctcgctcaag
     1381 aaagatggat catcgacggt ggtttttcca gaaagaaaat gcgcaagcgc aaggcgagcc
     1441 aagtttgtgc gtccatgggg atggatgtag agcgtgtcgc tttcaatttt tgtagaagag
     1501 aaaaattgca gtagcgcgtt gttcaaaagc agtgcatgcg ttgccagcgc caagcttaca
     1561 tttgcttctg caatttcttt gagcgagaga agataaaact gcaacgccgc accgcccgca
     1621 aaaattgttg cagatttttt ttcattgaac accgaagtat cgagtgggac tgcaagtcct
     1681 aagtggacac atttctcccg ccacgacagg aaatcgtttg gggcgcaaat aacttcgctg
     1741 cgttcaaacg cgctttctaa ataccgcttt cggaaatcgg caacgtgcgc cagatactca
     1801 ttgactgcat tggtatccat ggtgaccttg taacaaccgc tacagaatgc gatagtttgg
     1861 caagtcagaa ttttacatct ttttatcttt tgtgagcaag tgtttatttt atcgttcatc
     1921 cattatcttc taaataagca cccacgaatc gcgtgtaaca ccggcgctgc acttttacaa
     1981 gttggagcgc gtaagctgcc aaggataact attccaattt tttggattat gtcgcattgg
     2041 gggataacta tccccgcgag tttcgagtgg tcaaaatctg agtaaaattt ggggttactt
     2101 tccaaacaaa aaggaaatat tatagatatt ttgaccttac actacatacg cgtgtttggc
     2161 gcatgctaaa taaaaagtga caaatacagg gcaaggtagc ctcaatggct ctttggatgt
     2221 tgctgaaatt tgtgtgacat ggtggggaat gcaaccgctt atctgtccca ccggcggggt
     2281 gtggtattaa atggcgttag ttaatgataa tcccccacca acgcatcgat aatatcagag
     2341 gcaatcatac cacgcgtgcg gtttgttttt ttgtaccacc tctccgccgt tgaggcgtgt
     2401 gaatacacag cggctgatgc cgcttcgagg ggcggcattg tggcaatgag tgcaccgcac
     2461 atccccgcaa gaacatcgcc cgaaccggcg gtggcgagaa gagggttggc aaaggggttc
     2521 acgcggcaag tgtgtggatc ggcgatgagc gtgtgcgctc ctttcaaaac gaccgtagcg
     2581 ttggtgaggc gtgcggcttc ctttaccgca gcgaagcggt tactctcgat ggcggtggcg
     2641 gttgttttaa gtaggcgcgc aagttcgccc gagtgcggcg ttaatatttt ttgagcgggc
     2701 gattttttga gcgtatctac attttcggca accagagtga gcgcgtcggc gtcgaggaca
     2761 agcggtgcgg aggagtgctc caaaagataa agcacgactt tttttgcatc agcgtcaagg
     2821 ccgatccccg gacccaccac aatactgtta cgtttagaaa gagcggactc taagctctcc
     2881 ttgatttttg tacgctcgat ggataggagc ataatctccg cagcgcgatt ggcgtattcg
     2941 tcccatgttt ctccccacgc ggcgatagtc gaaagccctg cccctgtacg caatgcggcg
     3001 agcgaagcca actgggcagc gccggtttta cccgatgaac ctgcgacgat aagtacatcg
     3061 ccagcgcgat gtttatacgt cgtggcggag cggggggtgt gaaaaagtgg gatatcccct
     3121 tcttctaaaa gatgcgccgt ggcgcctgtc tcttgtagaa gcatgccgtt cggaacaccc
     3181 agcgctaacg tgtggatagt ccctgcataa tctttccctg tgggggtgta gagcccgcac
     3241 ttggggtacg cgaatgtgag cgtgtggtgt gctttgaacg ccacgcccaa cacagcgccg
     3301 gtgtcggcgt cgatccccga ggggatgtcg agcgcgacac gcagcgcgtt cgtattgttc
     3361 gcatcacgaa tgatattttt ttgaacgccc tcgatggcgc gtgtaaggcc ggtgccaaag
     3421 agagcatcga tgacgatgtc cgcctcctta agtaaaagtt caactgttcc gtcgacgccc
     3481 aaatttagcc gatggatagt gactgttaag atatgctgca agagggtgta gtttgcctcg
     3541 gcatctttag aaaaatttgt cgcatccccc gtaaggagca ccttcacgtt cgcttcttcc
     3601 gcgaggtgac gcgccacgac gaagccatcg ccgccattgt tgccggtgcc gcaaaggata
     3661 acaatgttag gtttcttaga accgctgagg aaaaaagaac ggattttatc ggcggcgcca
     3721 cgtccggcgt tttccatgag gattaaactc gggatgcggt atttctcgat ggcgagtttg
     3781 tcgtaaaagc gtatttgttc gcggttgagg agagggcgca tgggatagcg tcaagtttta
     3841 tggtaaacac gcgctgcaac taatttatat aaaaagtatt tttcgtgtat ccgttgaaat
     3901 cccacttgtg caaaaagttt ttcaaaaagg gggagttttt ttacatccag cgcgcaaagt
     3961 aaattaaaac aaaaataaag aaatttgact atcgcgtggg caagaacgcg ccttttgccg
     4021 gtggggatgt taaaatcgac aaagagccac tccccatttt ttgaaagcgc tgtcgaaagg
     4081 cgcggaagaa ttttttcgag acttacgtcg tcgaagcagt cgaggatgaa tggcgtgacg
     4141 atgagatcgt acttttccaa cggcgcgttt tcaagcgcgg cgcaagtgaa gcggatgcaa
     4201 tgtgtgtgcg cgaaagtgcg cgtgcgcttt tgtgcgcgtt ccaccatcgc ctcggcaatg
     4261 tcgatgtagt ggtattcctc cgcagtatta ttttttaaaa gtgtggttaa aattttcccc
     4321 gtaccgccgc caagaactaa agcgcgcctt gcttttttga gttgcggtat aaaatatgtt
     4381 tgtgctttga aaagttttcc cacaaaaaaa aacgacgcca aaaaatcata aaatggcgca
     4441 ataaaattaa acccgcgttt gcggcgtgcg ttattatcct tcatgcgtta aaaaataata
     4501 acaacagccc aaacgcaaac aaccactcgg cggcgatgcg gtaacgttga cgaacaagaa
     4561 aaaatctatc aaaatagaga atcgctcccg gggtggcgca taacagcagt acataaaacg
     4621 cacaggctgc atcgatgggt gaagtttgaa agaaaaaata aatagcgagc gcgcacaaaa
     4681 aaagaacgga taaaaaagaa atagcgatca cccccttgcg gatgttttgc acaaaacggg
     4741 gacgcacgcc aaaaattaaa ttttctttgt ggtcgagtgc atgctctatc accgagttca
     4801 taaataagtt gaggagcgcg gcgaggagaa aaaaactaaa gaataaaata ggtaaagtat
     4861 ctatcgcacc acggtggttc aagaacggtg caaaccacac ccccatagcg taaataactg
     4921 ccaccgaaat ttctttaccg aagcgaactt taccccaata cgccaaaatt aaatgaaaga
     4981 gcgcaaaaat accaacgacg atgcctccca taaaaataat ttcacgcaaa tacacccacg
     5041 cgaggatggc acaaagtaaa gagacaatgc acattgtggt gaaaataatc ctgaagtgct
     5101 cgacatgaaa tttatgccgc acgttgaccg cgactttgcc taccttgcgc gcatccatga
     5161 gatggtcggc ggtgtacaca acccagacgg cagcgcagag gagaaaccac catgtgcctt
     5221 tcatcggggc gtgcaacact gttttagcaa acgcccccga acccaacacg ccgatagcga
     5281 tgtcgaggct caacacctct gtgtagaaaa aaaaacgctg gagtgctgtg ccgagcatcg
     5341 acggtcttta aggctgtggt ttgttttttt gtaaaaatgc aataaacccg cgcatcgatt
     5401 cgtcgagaat ttggtacacg ttctcaaatg catcgtcgcc atgcccccat ggatcgggta
     5461 ccgagagtcc ttttttgcca ttggcgtgaa agtcacgcat caaaaaaact ttttcttcgg
     5521 gaaagtgaaa ctctgtcaaa agattgtttc tgttatccgt atccatcgcc aagatataat
     5581 cgaacagttc ggcgtctttg gcgtggacgg ggcgactttt ttgtttttca atcgaaacac
     5641 cgtggcggcg ggcgacggcc aacgaaccgc tgtgcggttt ttcgccttgg tgccatgcgg
     5701 atgtccccgc tgaatcggcg tcgtaaaagg cgcttaagta taaatccgcc gtttgttttt
     5761 caaaaatacc ttgtgccaaa ggcgaacgac aaatattacc gaggcataca aaaagtaccg
     5821 actgcattat gcacccgcgt acgacatgag cactacaaag ccgccgatga cttggtcttc
     5881 gtctttttcg ctcgctgctt cgccacgccg ccataaatca aaaaagtaac ggcaaatgct
     5941 cgcacctgcc ggtgggatat
//
LOCUS       DAOMPD010000113         5957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5640_length_5957_cov_2.308370, whole genome shotgun sequence.
ACCESSION   DAOMPD010000113 DAOMPD010000000
VERSION     DAOMPD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_5957_cov_2.308370"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..718
                     /locus_tag="PLY93_05250"
     CDS             155..718
                     /locus_tag="PLY93_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68919.1"
                     /translation="MLIVDDDKGIRMLISQIAQSWGYETVDCDSAESAIAVLSKSKFN
                     IVLTDIKMGKMDGIEFAEKIRNKMPSVAVIIMTGNPTTKTARQSQEMGAIYYMQKPMN
                     MDDLGNTLSIAAAWNIGMLTDSAARRFLALKNENDRAPMARTQSIKTHIKKLIGGIGW
                     MEHLRDFVYGRDIEKNPLYVELKNYLS"
     gene            complement(996..1904)
                     /locus_tag="PLY93_05255"
     CDS             complement(996..1904)
                     /locus_tag="PLY93_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68920.1"
                     /translation="MIRNILTGLVFFGTAAFQPFFAASLYDALKSAAKNCTVDLRYAR
                     LSACANGEDAAVKAAMDGMGVAKALPDLAAAFANKDEKLSATAASYLYRAKDYLGDII
                     KNPKLVSAKTVDTLIKGLKDNQSYVSSYASQITTVLATLKKKDAALFAVLDKHKENAT
                     RNDGYRWAMYQGRLRVFGRIQKASRDTKHEYLADAALNAPEYMYDYSDRERKKICEWE
                     KGFLKAENPRHEAYAARVLVLRCLGDYIDLVLDRAEELKNEGQLAHHALREALTNFTF
                     TCKEYMGQPATGSVAQCARREVLLQP"
     gene            2054..2749
                     /locus_tag="PLY93_05260"
     CDS             2054..2749
                     /locus_tag="PLY93_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HRP68921.1"
                     /translation="MRAAFLILFLFSTIYGGNWETINNEDGIIVFRKSQPNSNFFIFK
                     GEGNIDAPLSRILSIVQDTRLMPRWVSSCVEARLIEKNYDYATLKTNINDYYQIIYGR
                     AAVPWPFRDRDYVLKGKIAYEPKDRSISIHLVNIEHNKQPRRANIERMRVLRIEFILI
                     PMSTTRTKIIFTVALDPGGNIPAWAVNYMSKNEPLKTIKFLRLIAAQNISDRKAEAMI
                     ERQLQYLDQHKKR"
     gene            2825..3445
                     /locus_tag="PLY93_05265"
     CDS             2825..3445
                     /locus_tag="PLY93_05265"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HRP68922.1"
                     /translation="MQTLALVAETQRGPSVPDLVHRLIPVHRKFEFINSTVWFSGLII
                     GIAFLFFRRKSACINYLRAGAIISILRGIFICITSLGPPEALAKNTPPIMAAFTLEKL
                     NWQMLLQQWIPLDVLWGGSGFSAAWLTQDLFFSGHTSSTLLFLLVLERRDLVWWLYFM
                     LHAIVVFALVMTHEHYSIDILGAYFVAYAICAWLKNRHFWISEEQK"
     gene            complement(4148..5737)
                     /locus_tag="PLY93_05270"
     CDS             complement(4148..5737)
                     /locus_tag="PLY93_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP68923.1"
                     /translation="MHDQNFDVIFIGSGIGSLAAASLLAQYEGKRCLILEKHWQIGGY
                     THVFKRKKKYVWDVGLHYVGQVAEGEQIRRAFDAVTEGRLKWNRMPDVFDKFVYPDGT
                     FDHKSGVENYRADLEARFPHEKDAIALYFHDIAEVLLWFQRTEMGKNLPLALRWLEPL
                     IRKESPFALMTTEKYLEQRFSDPKLRAIVSSQWGDFGLPPSLSAFCIHAILVNHFFNG
                     AAYPVGSAEAIADTIAPIIERTGGRLLSSHQVERILVEDGKAVGVRVKKLRGEGQDEE
                     IVFKAKTIISCAGVYNTYVKLLDDERTLDYKRRIIHFNARHPPVSHISVYIALKDSAK
                     TLGLHGENHWIYDTYDHEETYKNRNEWVKRGRPPMAYLSLPSLKDPKAEHDGHTAEII
                     SFAPYGEYAAWKDQPWKKRDDIYKQIKEKAAQLLIDFVDERYKGFADLVDFYEVSTPI
                     TTEHMTGHDQGAIYGLASVPERYDITASPWCQVRTPIKNLYLTGADSTSLGIAGALMG
                     GVATCTHLVKPWRLPKLLKLV"
BASE COUNT         1574 a         1471 c         1393 g         1519 t
ORIGIN      
        1 gactggtcga tgaaaacgta cgtttcttct tgccaaagcc ttacacagcg gatttattgc
       61 tcaaaacgat tcgacaggca ttggaaaccg acgccaaaga aaataatacg aggagaaatt
      121 aatgtcccaa cgagccatgt attctaatat caggttgctt atcgtcgacg atgacaaagg
      181 aattcgcatg ctgatatccc aaatcgcgca aagctgggga tatgaaacgg tcgattgcga
      241 tagcgcggaa tctgcaattg ccgttttatc aaaatcaaaa ttcaatatcg ttctgaccga
      301 cataaaaatg ggaaaaatgg atggcattga attcgcagaa aaaatccgga ataaaatgcc
      361 gtcggtggca gtaatcatca tgacgggaaa cccgacaaca aaaaccgctc ggcaatcgca
      421 ggaaatgggc gccatatatt atatgcagaa gcctatgaat atggatgatc tgggcaatac
      481 tcttagcatc gcggccgcgt ggaatatcgg catgctgaca gatagcgccg cgcgccgatt
      541 tctggcgcta aaaaacgaaa acgacagagc ccccatggcc agaacccagt cgataaagac
      601 tcatattaaa aaactcattg gcggaattgg ttggatggaa cacctacgcg atttcgtata
      661 cggaagagac attgagaaaa atcctctcta tgtggagttg aaaaattatt tatcctaaat
      721 tcttccaaga atttttgaat gacacttagg ataaattcgg aattatcagg cgaatcgaat
      781 ggcgcatatt ttttctacag agtaagttcc gaaatttttc gccctttgat cagttcgtta
      841 ttttacaaat cacttcagcg aataaaattt acccgctacg gcgccctggt tcgaccgaag
      901 acggaagctc gtgagccggt aatctctcct gtgtaaagcc tttaatacag aatgacaagt
      961 tgtggcttta aaggcctata ttttatagaa gccgctcatg gctgtaataa tacttcgcgc
     1021 cgcgcgcact gcgccacgct gcccgtcgcc ggctgtccca tatactcttt gcaagtgaat
     1081 gtaaaattcg tcaacgcctc gcgcaaggca tggtgcgcaa gctgaccctc attcttcaat
     1141 tcttcggcgc ggtcgagaac cagatcaata tagtccccga gacagcgcaa caccaacacg
     1201 cgcgctgcat atgcttcgtg ccgcggattt tccgctttga gaaatccctt ttcccattcg
     1261 caaatttttt tcctttcgcg atcggaataa tcgtacatat attcgggagc attcaacgcc
     1321 gcgtcggcta aatactcatg cttcgtgtcg cgagatgctt tttgaattcg accaaaaacg
     1381 cgcaaacgcc cctgatacat agcccaacga tagccgtcgt tgcgcgtcgc gttttctttg
     1441 tgtttatcga gaaccgcaaa aagcgccgcg tcttttttct tgagcgtcgc gagtaccgtc
     1501 gttatctgag aggcatacga cgaaacgtaa ctttgattat ccttcaaacc tttgataagc
     1561 gtatcgaccg tcttcgcgct cacgagttta gggtttttaa taatatcgcc cagataatcc
     1621 ttcgcgcggt ataaatagct cgccgccgtc gctgaaagtt tctcgtcttt gttcgcgaat
     1681 gcggcagcaa gatcgggtaa cgctttcgcg acgcccatgc cgtccatcgc cgctttcacg
     1741 gcggcgtctt cgccgttcgc gcacgcggat agcctcgcgt aacggagatc gaccgtgcaa
     1801 ttttttgcgg cgctctttag cgcgtcgtaa agagaagcgg cgaagaacgg ttgaaaagca
     1861 gcagtaccga aaaacacaag accagttaat atatttcgta tcatgcgtgg catattcgat
     1921 caatttcaat gcatctacgt gaagtcaata gaaaacacga tttttcttct tcgggaaatc
     1981 gcgtcagagc ctgtgcgcag aacgatagtt tttacgatag acagcttatg ctgttccagc
     2041 gtcacaggtc gccatgcgcg ccgcgtttct gattctcttt ctattctcaa caatatatgg
     2101 cggcaactgg gaaacgatca acaatgaaga cggcataatt gtttttcgca agagccagcc
     2161 gaattcaaat ttttttatct tcaaaggcga aggcaatatc gacgcgccgc tctcgcgcat
     2221 actctcgatc gtacaagaca cgcgtctgat gccgcgttgg gtgagtagct gcgtcgaagc
     2281 gcgcctgatc gagaaaaatt acgattacgc aaccttaaag acaaacataa acgactatta
     2341 ccaaattatt tatggccgcg ctgcggtacc ttggccgttt cgtgaccgcg actatgtctt
     2401 aaaaggtaaa atagcgtatg aaccgaagga cagaagtatc tcaatacatc tggtgaatat
     2461 cgaacacaat aaacaaccaa ggcgggcgaa tatcgaacgt atgcgcgtat tacggattga
     2521 atttattctg attccaatgt cgactacaag aacaaagata atcttcaccg tagcgctcga
     2581 tccgggggga aacattccgg catgggctgt aaactatatg tcgaaaaacg aaccgttaaa
     2641 aacgattaaa tttctacgcc tgatcgcggc acaaaatatt tccgatagaa aagcagaagc
     2701 tatgatcgaa cgacaacttc agtatctcga tcaacacaag aaacgctgaa tgcccgattc
     2761 aagaattacg tcgaacagaa atatcctgtt ctggcttgta ctgcactacg gcgcttatat
     2821 tattatgcag acgctcgcac ttgtcgctga aacacagcgc gggccgtcgg ttccggattt
     2881 ggtacatcga ctgattcccg ttcatcgaaa attcgagttc attaattcta cggtttggtt
     2941 ttcggggtta atcattggga ttgccttctt atttttccgg cgcaagagcg cctgcatcaa
     3001 ttatctcaga gcaggcgcaa tcatctctat tctcagaggc atctttatat gcatcacctc
     3061 tctcggtccg cctgaagctc tcgcgaaaaa cacgcccccg atcatggcgg cttttacgct
     3121 cgaaaaattg aattggcaaa tgctattgca acaatggatt ccgctcgatg ttctttgggg
     3181 gggtagcggt ttttctgcgg catggcttac gcaagattta tttttttcgg ggcacacgtc
     3241 ttcgacgcta ctttttctac tcgttttaga acgacgagac ctggtgtggt ggctctattt
     3301 catgttacat gccattgtcg tatttgcgct cgtcatgacc cacgagcatt attcgatcga
     3361 tattctggga gcatactttg tcgcgtatgc gatttgcgcg tggttgaaaa accgccattt
     3421 ctggataagc gaagaacaga agtgagttac gaaaacgctc tctgcatttt gtagtcgtgc
     3481 accacagacc aatagagcat cgcaatcaat acggtttcaa tttaaacaga gagcaaaaac
     3541 agttacgcca aaagtggagc agactcgcct acgccttgct tactcccagg acgaggacgc
     3601 aaataacacg taacatatct tcatataatt ctacccgcac catagtttag tccgacttgc
     3661 gttacttgag tcaaaacgca ggtcaaaaac agcgggctac ggctcgcgca gtgtgtacaa
     3721 ggaaggcaat tcaaaggaac gaaggggcaa acaaacccat ttcaggctac ggcaactaaa
     3781 ttttcgatag aaaattggct ttacataccc cccctctcaa cctattggtg tgcatatcca
     3841 agtattatat atacagcagc attacaattg catcaatgaa tgcgagattc gtagcggata
     3901 gagcgatgga ggaattgtgc ccggatctgt attctcagac gtgcggtgtt ggcccagatg
     3961 gtccgttcta cggcattact caggaatggc ataagctgct taacacgacc agggagttgt
     4021 aaaaaaaagt gcggctgagc ggtactgtgt acaacgagac tgtagaaaaa ggctcccccc
     4081 tatagctacg agttcaaatt ctttcataat aaaccttcgc aacattcaaa gtcgtgaggt
     4141 aattaggtta cactaatttc aaaagtttcg gcaagcgcca cggcttcacc aaatgcgtac
     4201 acgtcgccac gccgcccatg agagcgcccg cgatacccag cgaagtcgaa tcggcgcctg
     4261 ttaaatacaa gtttttaatc ggcgtgcgca cttggcacca cggcgacgcc gttatgtcgt
     4321 aacgctcggg cacgctcgcg agaccgtaga tagccccttg gtcgtgcccc gtcatgtgtt
     4381 cggtagtgat cggcgtcgag acttcgtaaa aatcaacgag gtcggcaaaa cccttatagc
     4441 gctcgtcgac gaaatcgatt aaaagttgcg ccgctttttc tttgatctgc ttatagatgt
     4501 cgtcgcgttt tttccacggt tggtctttcc acgcggcgta ttcgccatac ggcgcgaatg
     4561 aaataatttc ggcagtgtgg ccgtcgtgtt cggctttcgg gtctttgagc gacggcaacg
     4621 acaagtatgc catcggcggc ctgccgcgtt ttacccactc gttgcggttc ttatacgttt
     4681 cttcgtggtc gtaagtgtcg tatatccaat ggttttcgcc atgaaggccc aacgtctttg
     4741 cgctgtcttt gagcgcaatg taaacgctga tatgcgatac aggcggatgg cgcgcgttga
     4801 aatggataat acgccgtttg taatcaaggg ttcgctcgtc gtcgagtaat ttcacatagg
     4861 tgttgtaaac tcccgcgcat gaaatgatcg tcttcgcctt gaagacgatt tcttcgtctt
     4921 gcccttcgcc gcgcaatttc ttgacacgca ctcccacagc cttaccgtct tcgactaaaa
     4981 ttcgctcaac ctgatgactc gacaatagcc gcccgcccgt tctttcgata atgggagcga
     5041 ttgtgtcggc gatagcttca gccgagccca cggggtatgc ggcgccgtta aagaagtggt
     5101 tcaccaaaat cgcgtggatg caaaaggcgc tgagcgacgg cggtaatcca aaatcgcccc
     5161 attggctcga tacgatggcg cgcagcttcg ggtcgctaaa gcgttgctcg agatattttt
     5221 ctgtcgtcat aagcgcaaac ggcgactctt ttcgaatcaa cggttctaac caacgcagcg
     5281 ccaacggcag atttttgccc atctcggtgc gttgaaacca caataggact tcggcgatgt
     5341 cgtgaaaata gagcgcaatc gcgtctttct cgtgcggaaa gcgcgcttcc aaatcggcac
     5401 gataattttc aacgccgctt ttatgatcga atgtgccgtc ggggtacaca aatttatcga
     5461 acacatcggg catacgattc cacttcaggc ggccctcagt caccgcgtcg aacgcacggc
     5521 gaatctgttc accctcggcg acttggccga catagtgcag accgacatcc cacacatatt
     5581 ttttcttgcg cttaaaaacg tgcgtgtagc cgccgatttg ccagtgcttt tcaagaataa
     5641 ggcaacgctt accctcgtat tgcgctaaga gactggccgc ggcgagcgag ccgatacccg
     5701 aaccgataaa gataacgtcg aaattttgat cgtgcatgca ccagcggctc acttttcatt
     5761 atatggacaa gcaaaatcgc gatagctggt atgtccatgg ggtagagcct gtgcgccaac
     5821 tcgggtagcg tatataaaga agcaagccgc ttaccctgat gcgtcatcta agaagcagaa
     5881 gtcgctcaat ttaggtactg atcgaccttg acggtaaata taaaatcctc ctgaacggta
     5941 atcccccgtt ttgttga
//
LOCUS       DAOMPD010000114         5946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5659_length_5946_cov_2.803938, whole genome shotgun sequence.
ACCESSION   DAOMPD010000114 DAOMPD010000000
VERSION     DAOMPD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5659_length_5946_cov_2.803938"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..848
                     /locus_tag="PLY93_05275"
     CDS             93..848
                     /locus_tag="PLY93_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68924.1"
                     /translation="MRQMNKKAMRIYTLNQNKFLALVAFIPLMLLLACRPATERAYQK
                     SFELRSAREVYTAGDTASARYYLNRVSSDTIVKEEAAAFLHRIDERKEHSDDCLADKK
                     AELELNRYPKMRYRHLFQIGVCFEVANNTKQAMGFYNLAESSGGKQPQLYIRRGLLRE
                     RLGDIKGASEDFIRADKLNHEYPPALLALALFQVRTGDVASATKTLAAIKDIKPTYAE
                     VIADALKHQKEYAAFPRGIAVGGIIKEEKDGTK"
     gene            835..1461
                     /locus_tag="PLY93_05280"
     CDS             835..1461
                     /locus_tag="PLY93_05280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP68925.1"
                     /translation="MAQSKSIRLWVLRGIALCLMLATGYIAWYTMESGKPRDFWQDKL
                     ATRANLQKFDGVFEDQNGKQVKLSEFAGKNAIATFVFKNCTMVCPTIMNDLRLFDKDY
                     PDFKAKGNFLVFTFEDHRGHADDLKDFLKKYSVDGNQWHVLTSDAATIKRLADTYDLQ
                     YQKEADGKVIYAHSNVFLVADTKGKIRREFRGIENNKARFFDEIKKFL"
     gene            1458..1823
                     /locus_tag="PLY93_05285"
     CDS             1458..1823
                     /locus_tag="PLY93_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68926.1"
                     /translation="MIKKHKGTFIALLLGMWGLFAANGIKDFYFEMRMKARGEIISYA
                     SLQEFCGWTGDQKPGETEMEYAWRRARNMFSAIMVISFMSVLLFGAIFGIIFAIFWWS
                     GAFRDDDDEYEQQQAKGKV"
     gene            1820..2593
                     /locus_tag="PLY93_05290"
     CDS             1820..2593
                     /locus_tag="PLY93_05290"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRP68927.1"
                     /translation="MKFSTKLWVLVSVVLTLAAATIKWYFLPMTEKYAELTIRDQMKG
                     EMQSFEKILGLIAYTADKDKAFVKRAWSLIAEDKSIPYEFRRSDFLHAQYGFKENRAA
                     QNDFEKKVLASGNAEFQSDKDFITYAYPLKAQKVCQGCHFDKAGKAIELGKPLGLVVH
                     KVPVTTITESPAAYFSLNLFWENFGLVMLCIALAMFPIWLWILKPIRQVATQSEEILR
                     HADERGESDTIGDFDDNRPKKVVDELDAIRRLVDYAKRR"
     gene            2577..3605
                     /locus_tag="PLY93_05295"
     CDS             2577..3605
                     /locus_tag="PLY93_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRP68928.1"
                     /translation="MQSVVNTDTRAIRRWLYACATMVFLIVVVGGITRLTRSGLSIVE
                     WKPISGTLPPLNDTEWNIEFSKYQETPEYKIINHKMTLSEFKDIFWWEFIHRTIARLI
                     GVVFFFPFVYFLLRRRLSAKLAIKLLLIFSIGALQGFMGWYMVKSGLVKDPHVSHLRL
                     MLHLMLAVLLYMLLLTTSWRLKWRRDPNQKASAEHATMMAAFFILATGALVAGLKAGK
                     MYNTWPLMNGAYYPPGIEYMTPWYLNLYEHPALVQFIHRNAAYAFGAWLLVFCYRRRR
                     EIFAAPLRHPLTWLVVVYALQVLLGVLTLLQVVPVGLAAKHQMNALILFTVALYMFIR
                     RQSVADKV"
     gene            3642..4091
                     /locus_tag="PLY93_05300"
     CDS             3642..4091
                     /locus_tag="PLY93_05300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRP68929.1"
                     /translation="MQILTEIEINADADKIWQTVVDFAAYDSWNPVIQKVTGVPHKGD
                     MVSFSVVAFPGVLLPIVACKVLVADPKERHLRWKGPDMNVVKDILSGEHFFIVQEKAP
                     GKCRFIHGENFTGLVAPLLRPLLETRITSLYDQMNQALKVRCEGYST"
     gene            4098..5300
                     /locus_tag="PLY93_05305"
     CDS             4098..5300
                     /locus_tag="PLY93_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP68930.1"
                     /translation="MQGIRIKDYKAGSAVVIQNAPNPGIFYIVRKGILSIDTEHRLND
                     KVLSRFEEGDSFGLVSALTGHHFLVTIYAVTEAQVAEIPVSMIGGYLKNQSELAMKVL
                     RLYSRELRTIQLHLSKLDNSDARNITPDVLYNLAQKYIEWGKPHYAARALHAYIPWAE
                     KNNGVYLDLARAVWPNIESHFQEIPVGTKASRIQSDTMIFSEGDPGREIYVVLTGAVK
                     IMRLVHGNEFIVDVLGAGELFGEMAFIENAPRMGSAITTQESTLVRVLPQQLIDSVGE
                     GVLQKIFENMARRIWFSHQRLIIFKINDPVVRLHAHLYNLIRHQNLRTQGDSPQDKSY
                     TFQITFAELKTLCGIMRMKESVENTFLNDNNLVVDETSITIKSRKKLEDQIAYYRAKS
                     GQIISVTK"
     gene            5310..>5946
                     /locus_tag="PLY93_05310"
     CDS             5310..>5946
                     /locus_tag="PLY93_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRP68931.1"
                     /translation="MTHDHHSHSHSSHHAHEHGRVGKNLAIGVVLNFLFAIVELVGGL
                     LTNSVAILTDALHDFGDALSLVVAWVLQKISEKPRDAKFSYGYKRFSLMGTVFISTIL
                     AVGAIVMITESVKRFLAPQAVDAKGMLWLAVFGVLINGLAALRMKRGTSFSERAVFLH
                     FMEDVLGWIAVLIGSVVMLFYPAPWLDPLLSMGIALWVLYNVVRNLNQVGRI"
BASE COUNT         1547 a         1396 c         1446 g         1557 t
ORIGIN      
        1 attggaattt aaaggcgcag aggttaaact tttagactat gaacctaacc aagttaaatt
       61 tttcttgcgt accgcgccgg cggcgagcgg aaatgagaca aatgaataag aaagcaatgc
      121 ggatttacac acttaaccaa aataaatttt tagcgcttgt agcgtttatc ccactgatgc
      181 tcttattggc ctgccgaccg gctaccgagc gcgcatacca aaaaagtttt gagctgcgtt
      241 cggcgcgcga agtgtacacc gctggcgata ccgcaagcgc gcgttattat ctaaaccgtg
      301 tgtcgtccga taccattgtc aaagaagagg cggctgcatt tttacaccgc atcgacgaac
      361 gcaaagaaca cagcgacgat tgtctggccg ataaaaaagc cgagctggag ctcaaccggt
      421 atcccaaaat gcgttatcgg catctctttc aaataggggt ttgtttcgag gttgcaaaca
      481 atacgaaaca agcgatgggc ttctataact tagccgaaag ctctggcggt aaacaaccgc
      541 aactctacat ccgccgtggt cttttgcgcg aacgcctagg ggacattaaa ggcgcatccg
      601 aagattttat tcgcgcggat aaactcaacc atgagtatcc ccccgctctt ttagctctcg
      661 ctctttttca agtgcgtacg ggtgatgtag cgagtgcgac aaaaaccttg gcggcaatca
      721 aagatataaa acccacgtac gccgaagtca tcgccgatgc gctcaaacac caaaaagaat
      781 acgcggcgtt cccacgcggc attgctgtgg gcggcataat caaagaagaa aaagatggca
      841 caaagtaaaa gtattcgact ctgggtttta cgcggcatcg cgctctgtct tatgcttgcc
      901 accggttaca tcgcgtggta cacgatggaa tcgggcaagc cgcgcgattt ttggcaagac
      961 aagcttgcca cacgcgccaa cctgcaaaaa tttgacggcg tctttgaaga ccaaaacggc
     1021 aagcaggtta agctctccga gtttgccggt aaaaacgcga tcgctacttt tgttttcaaa
     1081 aattgtacga tggtgtgccc cactattatg aacgacttgc ggctattcga taaagactat
     1141 ccggatttca aggcgaaagg taattttttg gtctttacct ttgaagacca ccgtggccac
     1201 gccgacgatt taaaagattt tttgaaaaag tatagtgtcg acggtaacca gtggcatgtt
     1261 ttgacatcgg acgcggcgac aatcaaacgt ttggccgaca cttacgattt gcaataccaa
     1321 aaagaggccg acggcaaagt gatttatgca cacagcaatg tctttttagt cgcagatacg
     1381 aaaggaaaaa tccgccgcga gttccgtggc attgaaaaca acaaagcgcg ctttttcgat
     1441 gaaataaaaa agttcttatg ataaaaaaac acaaaggcac ttttattgcg cttctcttgg
     1501 gcatgtgggg tctttttgcc gccaacggca ttaaagattt ttattttgag atgcgcatga
     1561 aagcgcgcgg tgaaatcatt agctacgcgt cgctgcaaga gttttgcggt tggacgggcg
     1621 atcaaaagcc cggcgaaaca gaaatggagt atgcctggcg gcgcgcgcgc aatatgtttt
     1681 ccgccatcat ggtgattagt tttatgtctg tgcttctctt tggcgctatc ttcggtatca
     1741 tcttcgctat tttttggtgg tcgggcgcat tccgcgacga cgacgatgaa tacgaacaac
     1801 aacaagcaaa aggaaaagta tgaaattttc aacaaagtta tgggtgttag tgagtgtggt
     1861 cctcacgctg gcggcggcga cgatcaagtg gtatttttta cccatgacag aaaaatacgc
     1921 agagctaacc atccgcgatc aaatgaaagg cgaaatgcaa tcgtttgaaa aaattttagg
     1981 tctcatcgcg tataccgccg ataaagacaa agcgttcgta aagcgcgcgt ggagtctcat
     2041 cgccgaagat aaatcgatac cgtatgagtt ccgccgtagc gatttcttgc acgcacaata
     2101 tggctttaaa gaaaaccgcg cagcgcaaaa cgattttgaa aagaaagtat tggcatcggg
     2161 aaatgctgaa ttccagagcg acaaggattt tatcacgtac gcgtatccgc tcaaagcgca
     2221 aaaagtatgc caaggatgcc atttcgataa agccggcaag gcgattgagt tgggtaaacc
     2281 cttagggctc gttgtgcata aagtaccggt gacgacaatc accgaatccc ccgcagcgta
     2341 tttttcgctc aacctctttt gggaaaattt tggattggtg atgctttgca tcgcattggc
     2401 aatgttcccc atctggcttt ggatactcaa acctatccgt caagtggcta cacagagcga
     2461 agaaattttg cgccacgccg acgaacgcgg tgaaagcgac actatcggcg actttgacga
     2521 caatcgtccc aaaaaagttg tcgacgaatt agacgcgata cgccgtcttg tggattatgc
     2581 aaagcgtcgt taatacggat acccgcgcca tccgtcgctg gctttacgcc tgcgcaacaa
     2641 tggtgttcct catcgtcgtt gtgggtggaa tcacccgcct aacccgttcg gggctttcaa
     2701 ttgtcgaatg gaaacccatt tcaggcacat taccgcccct caacgacacc gagtggaata
     2761 tagaattttc aaaatatcaa gaaacacctg agtacaaaat catcaaccac aagatgacgc
     2821 tatccgagtt caaagatatt ttttggtggg agttcatcca ccgcacgatt gcgcggctta
     2881 tcggtgtggt gttctttttt ccgtttgtgt attttcttct ccggcgaaga ctatcggcga
     2941 agttagccat caaacttttg cttatttttt ctatcggcgc tctccaagga tttatgggtt
     3001 ggtacatggt gaaaagcggt ctcgtgaaag atccgcacgt aagccatcta cgccttatgc
     3061 tgcacttgat gcttgcggtg ctgctctata tgttgttact cacaacgtcg tggcgtctca
     3121 aatggcgccg cgatcccaac caaaaagcga gcgccgaaca tgcaacgatg atggcagcgt
     3181 tttttatttt agcaaccggt gcgctcgttg caggacttaa agcaggtaag atgtataaca
     3241 cgtggccgct catgaatggc gcgtattatc cccccggcat tgagtatatg accccgtggt
     3301 atttgaatct ctatgagcac cccgcgcttg tgcaattcat ccaccgcaat gcggcgtacg
     3361 cattcggcgc atggctttta gttttttgtt accgccgtcg ccgtgagatt tttgcggcgc
     3421 cgctccgtca tccgttgacg tggttagttg tcgtgtacgc actgcaagtt ctcttgggcg
     3481 tgttgaccct gcttcaggtt gtgccggtgg gtttggccgc aaaacaccaa atgaacgcgc
     3541 tcattctttt taccgttgcg ctctacatgt tcatacggcg gcagagtgtt gcggacaaag
     3601 tttagagttg tcgcatagac tctctctttc gtgaagggtg catgcaaata ctgactgaaa
     3661 ttgagattaa cgccgacgcc gataagattt ggcaaacggt tgttgacttt gcggcgtatg
     3721 attcatggaa tcctgtcatc caaaaagtga ccggtgtacc acacaagggc gacatggtta
     3781 gttttagcgt tgtagcattc cccggtgtac ttttaccgat tgtcgcttgt aaagtgcttg
     3841 tcgccgatcc caaagagcgt catctgcgct ggaaaggccc cgacatgaat gtcgttaaag
     3901 atattttaag cggggagcat ttttttatcg tacaagaaaa agcccccggg aaatgccggt
     3961 ttatccacgg tgaaaatttc accggccttg tggctccgct tttgcgtcca cttttagaga
     4021 cacgcatcac tagcctctac gaccagatga accaagcgtt gaaagtgcgt tgtgaagggt
     4081 attcaaccta aacgcacatg caaggaattc gcattaaaga ttacaaggcc ggttcggctg
     4141 ttgtcatcca aaatgctccc aaccctggta ttttttatat cgtgcgcaag ggtattttgt
     4201 ctattgacac cgagcaccgg ctcaacgaca aagtgctttc gcgttttgaa gaaggcgata
     4261 gttttggttt agtttcggct ctcaccggtc atcacttttt ggtgacgatt tatgctgtca
     4321 ccgaagcgca agttgccgaa attcctgtgt cgatgattgg aggctatctt aaaaatcaaa
     4381 gcgaattggc gatgaaagta ttgcgtcttt attcgcgtga gttacgcaca atccaattac
     4441 acctgtcaaa actggataat tcggacgcgc gcaacatcac ccccgacgtt ctctataact
     4501 tagctcaaaa atatattgaa tggggcaagc cccattacgc cgcacgcgcg ctgcatgcgt
     4561 atatcccttg ggcagagaaa aacaacggtg tttacctcga tttagcgcgc gcggtgtggc
     4621 cgaacatcga atcccatttt caagaaatcc ctgtgggcac aaaagcaagt cgaatccaaa
     4681 gcgatacgat gattttttcg gagggcgacc ctggccgtga aatttatgtc gtacttacgg
     4741 gggctgtcaa aattatgcgc cttgtccacg gtaacgagtt tattgtcgac gttttgggcg
     4801 caggcgagct ttttggtgaa atggcgttta ttgaaaacgc gcctcgcatg gggtcggcaa
     4861 tcaccacaca ggaatcgacg ttggtgcgtg ttttaccgca acagcttatt gacagcgttg
     4921 gcgaaggcgt gttacaaaaa atttttgaaa acatggcgcg ccggatttgg ttttcgcacc
     4981 aacggcttat cattttcaag ataaacgacc ctgtggtgcg tttgcacgcg catttatata
     5041 acttaattcg gcaccaaaat ttgcgcacac aaggcgatag cccccaagat aagtcgtaca
     5101 catttcagat tacctttgca gagttaaaaa ctttgtgcgg cattatgcgc atgaaagaat
     5161 cggttgaaaa tacattcttg aacgataaca accttgtcgt tgacgaaacc agtatcacaa
     5221 tcaaaagccg caagaaactc gaagaccaaa ttgcgtatta ccgcgccaag tcagggcaaa
     5281 ttatttcggt gacaaagtaa aaaaaaaata tgacgcacga ccatcacagc cattctcact
     5341 ccagccacca cgcgcacgag catggtcgag tgggtaaaaa tctagcaatc ggcgttgtcc
     5401 tcaatttttt atttgcaatc gtcgaactgg tgggtggtct tttaaccaac agtgtcgcca
     5461 ttttaaccga cgcgttacac gattttggcg atgcgctctc ccttgttgtt gcttgggtgt
     5521 tgcaaaaaat ttcagagaaa ccccgcgacg ctaaattctc gtatggttat aagcgtttct
     5581 cgctcatggg aaccgtcttt atttccacaa ttcttgccgt gggagcaatt gtgatgatca
     5641 ccgaatcggt gaaacgcttt cttgcaccgc aagcagtcga cgcaaaaggt atgctgtggc
     5701 tcgccgtctt cggcgtgctc atcaacggtt tggctgcgct gcgtatgaaa cgaggcacca
     5761 gtttttcaga acgcgctgtg ttcttgcatt ttatggaaga cgtgctggga tggattgcgg
     5821 tactcattgg cagcgtcgtg atgctttttt atcctgcgcc atggctcgac cctctcttgt
     5881 cgatggggat tgctctctgg gtgctctata acgtcgtgcg taatttaaac caagttgggc
     5941 gtattt
//
LOCUS       DAOMPD010000115         5937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5667_length_5937_cov_2.208092, whole genome shotgun sequence.
ACCESSION   DAOMPD010000115 DAOMPD010000000
VERSION     DAOMPD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5667_length_5937_cov_2.208092"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>576)
                     /locus_tag="PLY93_05315"
     CDS             complement(<1..>576)
                     /locus_tag="PLY93_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP68932.1"
                     /translation="DEPTNHLDAESVEWLERHLQEYEGTVIAITHDRYFLDNIAGWIL
                     ELDRGEGIPWKGNYSSWLEQKSNRLANEEKAESKRRKTLERELEWVRMGAKGRQAKSK
                     ARLGAYEKLLNEDAKEKESRLEIYIPDGPRLGDKVIIAEGISKSFGDKLLYENLSFEL
                     PPNGIVGVIGPNGAGKTTLFRLILSAAASHG"
     assembly_gap    577..590
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<591..1147)
                     /locus_tag="PLY93_05320"
     CDS             complement(<591..1147)
                     /locus_tag="PLY93_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP68933.1"
                     /translation="MADDKKIIFSMVDVSKVYPPHKQVLKDIHLSFFYGAKIGIIGLN
                     GSGKSSLLKIIAGILKPDKGEVIFSPGYSMGYLEQEPKLDDTRTVRAVVEEGVGEVVK
                     LLKEYNVVSESLGHDLSDDEMDKAIERQGELGELIEKANGWDLDQTLSRAMDALNCPD
                     ENSLIKNLSGGEKRRVALCRLLLQKP"
     assembly_gap    1249..1317
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1318..2223)
                     /locus_tag="PLY93_05325"
     CDS             complement(<1318..2223)
                     /locus_tag="PLY93_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68934.1"
                     /translation="MITPIKRNLIILKDAFRQSDAPAKRRFFIFFILTTFTVFTSGLT
                     FSIQSTTQGRYTDAALYTLALILIMSVHAFSRFVQARSYGVYSALPLFIPMPLFSPFG
                     TIGVATRTASVGLNARALFDIAFWGPFFTFILSVVFLFIGVLQSDIVPIKSQFENPLI
                     LTGILALLKDIPPGYDVAMHPLTVAGWAGLLFTAINLFPLGSLSGGQIAYALFGRRQR
                     DIGYLFMAALFIMALYYPLWFAFVIAFIYLGVEHPAVRQAVNPLFSDFNPRLSENSLD
                     RRRQLFALLSACIFAVSFTLTPFGAS"
     assembly_gap    2357..2366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2367..4491)
                     /locus_tag="PLY93_05330"
     CDS             complement(<2367..4491)
                     /locus_tag="PLY93_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRP68935.1"
                     /translation="MKYGATLLISTILLALPVMRKIPRFTLSQVAEKDLKASMDLKIV
                     DTAETERLKKMAYERERPAFDRDAQRFNTIQQQLQDDFSWVIKSIMKSQTLSTDKRIE
                     LLKERFPFFKNKNYRLSDIRSLLTEKKIALLDEKASYLAQKSFNTRGYLDKNLAPAIY
                     NELREKGGQIRTVGSSQEIPDVIWSIEQVQSIDAPFSRLRFSAPERDLKNSTLRIILT
                     RIDELMRENAYLTYNPQYTELRRRQAADKVTPVVKHIKHGTILLHAGDVIDAERLKFL
                     EQVRDNHRRYNGAQVLGIFLVLSVLAVSICYFTFRFAYSQVRDYASHFILHGLFWFMF
                     VVALLTMYTNPLRAYDVHFILFVPFGFFGILTGQLFGARIALSSGIYLSIFSYILAGF
                     DNQSLLLSLTSVIAGLYASTRMETRSQMFKGGIIITVTNLVIIAGFELMTPASRNFDL
                     KVAAVIANSLISVLLTFGVLPLLEYIFNMPTPFRLMELNDFNHPLLTRMAAIAPSTHS
                     HSVMLANLSEAAVRSVGGDTLLTRVGCLFHDIGKMVHPDFYAENRHLYPTSESFKKLG
                     HLKSAQMIIRHVTDGIAMARENRLPEKVIRYIPEHHGTTTIQYFYHKALEEAGKAQGQ
                     ISRKYFQYPGPKPQSKETALVMIADSVEAASRTATSATPAEFEQIIDRIIENKISEEQ
                     FHECSLTLGDLARARKAFLDVLVSTY"
     gene            complement(4579..5064)
                     /locus_tag="PLY93_05335"
     CDS             complement(4579..5064)
                     /locus_tag="PLY93_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68936.1"
                     /translation="MGDAKKSKKKSDETVYTESDVLARIETVLRFTSKELANSETEAQ
                     AFEKLAEFEAQLLEQQRSVSAALENVQKYRDEIQKDWDKEKLVHETALDFMATRMKDL
                     YETLRAQEYLLDYAANEQKFLRVLLIELREKRQLNATDIENILARHEALLNELSRKAK
                     K"
     gene            5122..5850
                     /locus_tag="PLY93_05340"
     CDS             5122..5850
                     /locus_tag="PLY93_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68937.1"
                     /translation="MLEKVIIPEKFKSAVDLAIQNEHLIRIRAYAVISATEAGLNYIV
                     ESILQRYNREDLLGPVYTSVKELSMNGAKANFKRILFEDESLNSEDDAEYERGMQLFK
                     EKLNETWVLECGRKAKERKLYVDIFFDFNADRLIVEVNNNRPISPKEDKRIREKFLAA
                     RRYDDIAQFYIEGGDSSEGAGMGIVLVTMMLKAQDIDPHLFTIRSDYREKTLARVEFP
                     LSPDYKPQRERFINEAVVEPVLSI"
BASE COUNT         1542 a         1260 c         1424 g         1618 t
ORIGIN      
        1 aaccatggct tgcggcagcg gacaaaatta aacgaaagag tgtcgttttc cctgcaccgt
       61 tggggccgat gactcctacg ataccgttgg gcggtaactc gaagctcaaa ttttcgtaga
      121 ggagtttatc gccaaaagat ttacttattc cctcggcgat aatcacttta tcgcccaagc
      181 gcggaccatc tgggatataa atttctaaac gactttcttt ttcttttgcg tcttcgttca
      241 agagtttttc atacgcaccc aaacgcgctt tggattttgc ctgacgtcct ttcgcgccca
      301 tgcgcaccca ttcgagctcg cgctctaaag tcttgcgccg cttcgactcg gctttctctt
      361 cgtttgcgag gcggttcgat ttttgttcca accacgaaga gtagttgcct ttccaaggga
      421 tgccttcgcc gcggtcgagt tctaaaatcc aacccgcgat gttatctaaa aagtaacgat
      481 cgtgggtgat ggcaatcacg gtgccttcat attcttgtaa gtgtcgctcc aaccactcaa
      541 ccgactctgc gtctaaatgg ttggtgggtt cgtccannnn nnnnnnnnnn ggcttttgta
      601 ataacaatcg acagagcgcg acgcggcgct tttccccacc tgataaattt ttaatgagag
      661 agttttcatc gggacagttc aaggcatcca tcgcgcggct taaggtttgg tccaaatccc
      721 atccgtttgc tttttcaatg agttcgccca actcgccttg gcgttcgatc gctttatcca
      781 tttcatcgtc gctcaagtca tggccgaggc tttcgctcac gacattatat tccttgagga
      841 gttttaccac ctcgcccacg ccttcttcaa caaccgcacg cacagttcgg gtgtcgtcga
      901 gtttaggttc ttgttctaga taacccatcg aataccctgg ggaaaaaata acttcacctt
      961 tgtcgggctt taaaatgcca gcgataattt ttaagagcga agacttcccc gaaccgttca
     1021 acccgatgat gcctattttg gctccataga aaaacgaaag gtggatatct ttgagtactt
     1081 gtttatgcgg cggataaacc ttgcttacat ccaccatcga aaaaattatt tttttatcgt
     1141 cagccatttt tgcctctaaa ttgttactaa aaaattaaat tgagttttcg ctattggaag
     1201 gcggtacgct ctcggggatt tgcggcgctt ggtatggttg gaaatcatnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct
     1321 tgcgccaaaa ggagtcagtg taaaagaaac ggcgaagatg caagcgctca ataaggcgaa
     1381 gagttgccgc cgtcgatcga gagagttttc agataagcga gggttgaaat ctgaaaaaag
     1441 aggattcacc gcttggcgta cggcggggtg ttcgacgccc aaatagataa aggcaatcac
     1501 gaaagcaaac cagagtgggt aatagagcgc cataataaaa agcgcagcca taaaaagata
     1561 accgatgtcg cgctggcgtc tgccgaaaag tgcatacgcg atttgccctc ccgacaacga
     1621 acccaaaggg aataaattaa ttgcagtaaa aaggagtccc gcccaaccag ccaccgtaag
     1681 tgggtgcatc gccacatcgt accctggagg aatgtccttg aggagtgcaa gaatgccagt
     1741 gagtataagc ggattttcaa attgactttt aatcgggacg atatcgcttt gcaaaacgcc
     1801 aataaaaaga aagaccacag acagaataaa tgtgaaaaat ggtccccaga aagcaatatc
     1861 gaagagcgca cgtgcgttga gtcccacgct tgctgtgcgt gttgccactc caatcgtacc
     1921 aaaaggagag aaaagaggca tcggtataaa cagaggtaaa gcggagtaaa cgccataaga
     1981 acgcgcttgt acgaatcgag aaaatgcgtg caccgacata atgagtatga gtgcaagcgt
     2041 atagagcgct gcatcggtgt agcggccctg agttgtggat tgtatcgaga aggtaagccc
     2101 agaggtgaag actgtaaaag ttgtcaaaat gaaaaaaata aaaaaacggc gttttgccgg
     2161 agcatcactt tggcgaaaag cgtctttgag gattattagg ttgcgtttaa ttggtgttat
     2221 catattagtt aataagttcg ggcgtcttag attttttctt gggtcgttta cgcttacttc
     2281 ctgtcctttt tttctggtcg gcttgtacag aaacttctaa agcattggtt tgtttcatac
     2341 tggggtagcg agggcgnnnn nnnnnnggta ggtgcttact aaaacgtcca agaatgcttt
     2401 acgtgccctt gctaaatcgc ccaaagtcaa agagcattcg tggaattgtt cttcgctaat
     2461 tttattttca atgatgcgat cgatgatttg ctcaaattct gcaggggtgg ccgaggttgc
     2521 ggtgcgcgat gcggcttcga cgctgtcggc aatcatcacg agcgcagttt ctttactttg
     2581 cggtttgggt cctggatatt gaaagtattt acgagaaatt tgtccttgtg ccttgcctgc
     2641 ctcttctaaa gctttgtggt aaaaatattg gattgtcgtt gtgccgtggt gttcggggat
     2701 atagcggatt actttttcag gcaaccgatt ttcgcgagcc atcgcaatcc cgtccgtcac
     2761 atgacggatg atcatctgcg ctgatttaag atgtcccagt tttttgaagc tttcagaggt
     2821 tgggtataaa tgtctattct ccgcgtagaa atcgggatgc accattttac cgatgtcgtg
     2881 gaagaggcaa ccgacgcgtg tgagtaatgt atctccaccg acagagcgca ccgccgcttc
     2941 gcttaaattg gcgagcatca cgctatgcga gtgagtggaa ggggcaatcg ccgccatacg
     3001 cgttaagagt ggatgattga agtcgttgag ttccatgaga cgaaacggtg tcggcatgtt
     3061 aaaaatatat tcgaggagag gcagcacgcc aaaagtgaga agaacgctaa ttaggctgtt
     3121 ggcgatcaca gcggcgactt ttaagtcgaa gttgcggctc gccggagtca tgagttcaaa
     3181 accagcgata attaccaagt tggtgactgt aataataata ccgcctttga acatctgcga
     3241 gcgggtctcc attcgagtgg aagcataaag tcccgcaatg acagaggtga gcgacaaaag
     3301 caggctttgg ttatcgaacc ccgccaagat ataggaaaaa atagagagat atattcccga
     3361 ggaaagagcg atgcgcgcac caaaaagttg accggttaag ataccaaaaa aaccaaacgg
     3421 tacgaataaa ataaaatgca cgtcgtatgc gcgtaatggg tttgtgtaca tcgtcaagag
     3481 ggcaacgaca aacataaacc aaaataatcc gtgtaagata aaatgcgacg cataatcccg
     3541 cacttggcta tacgcaaagc gaaatgtgaa ataacaaatc gagacggcga gtacgctcaa
     3601 gaccaagaaa attcccagta cctgtgcgcc gttgtagcgg cggtggttgt cgcgcacctg
     3661 ttcgaggaat tttaatcgtt cggcgtcgat gacatcgccg gcgtgtaaaa gaattgtacc
     3721 gtgttttatg tgttttacga ccggcgttac cttgtcggcg gcttgtcggc gtctgagttc
     3781 ggtgtattga ggattgtacg ttaagtatgc gttttctcgc atgagctcat cgatgcgcgt
     3841 taatataata cgcagagtgg agttttttag gtcgcgctct ggcgctgaaa aacgtagcct
     3901 ggagaaggga gcatcgatgc tttgtacttg ttcgatgctc caaatgacat cgggtatctc
     3961 ttgagaacta ccgacggtac gtatctgtcc acctttctcg cgtagttcat tgtagattgc
     4021 cggcgcaaga tttttatcca gataacctcg ggtgttaaaa gatttctgtg ccaggtaact
     4081 tgccttttca tcgaggagtg caattttttt ttcggtgagt aacgaacgaa tatcgcttag
     4141 tcgataattt ttattcttaa agaatgggaa tcgctctttg aggagttcga tacgtttgtc
     4201 tgttgaaaga gtttgacttt tcatgatgga tttaataacc catgagaaat cgtcttgaag
     4261 ctgttgttgt atcgtattga aacgttgcgc atcgcgatcg aaagccgggc gttcgcgttc
     4321 gtacgccatt ttttttaagc gctccgtttc tgccgtgtcg acaattttta aatccatgga
     4381 agctttaagg tctttttcgg cgacttgaga taacgtgaag cgtggaattt tacgcatcac
     4441 tggtaatgcc agtagaatag ttgaaatgag caaagttgcg ccatatttca tgctgcgaac
     4501 gcagttctga gaggaagcga ttgaactgta agaaaaatgc cctcagcttt tgtttgaagg
     4561 ggggagatgg cattacgttt atttttttgc tttgcgggag agctcattga gtaacgcttc
     4621 gtgccgcgct aaaatatttt caatatccgt tgcattgagc tgtctttttt ctcgaagttc
     4681 gatgagtaaa acacgtaaga atttttgctc gttggcggcg taatcgagga gatactcttg
     4741 cgcgcgcaat gtttcataca aatccttcat gcgcgttgcc ataaaatcga gcgcggtttc
     4801 gtgtacgagt ttttctttat cccaatcttt ttgaatttcg tcgcggtatt tttgcacgtt
     4861 ttccaacgcc gcgctgacgc tacgctgctg ctctaaaagt tgcgcttcaa attcagcgag
     4921 cttttcaaat gcctgtgctt cggtttcgct gttggcgagt tctttgctgg tgaagcgtaa
     4981 aaccgtttca atgcgcgcca aaacgtcgct ttcagtgtac actgtctcat cgcttttctt
     5041 tttcgatttt ttagcgtcgc ccatgcgcaa ctttccaaag gattgacaag gggtcaaaaa
     5101 gaataacccc gacgagtaag catgctcgaa aaagtaataa tacccgagaa atttaagagt
     5161 gcagtcgatt tagcgataca aaacgaacat ctaatccgta tccgcgcgta cgcggtcatt
     5221 tctgcaacgg aagcaggtct caattatatc gttgaatcga tactccaaag gtataaccgt
     5281 gaagatttgt tgggccctgt gtatacgtcg gtgaaagaac tatcgatgaa cggtgcaaaa
     5341 gctaatttta agcgtatttt atttgaagat gaaagtttga actccgagga cgatgctgaa
     5401 tatgaacgtg ggatgcagct ctttaaagaa aaattaaacg aaacctgggt gttggaatgc
     5461 ggcagaaaag ctaaagaaag aaaactctac gtcgatattt ttttcgactt taacgccgat
     5521 cgtcttatcg tcgaagtcaa taataaccgt cccatttctc caaaagaaga taaacgcatc
     5581 cgcgagaaat ttttagccgc gcgccgctac gacgacatcg ctcaattcta tatcgaaggc
     5641 ggcgactctt ccgaaggcgc aggcatggga attgttctcg ttacgatgat gcttaaagca
     5701 caagatatcg atccgcacct ctttaccatc cgttcagatt accgcgagaa gactctcgct
     5761 cgggtggagt ttccgctttc acccgattat aaacctcagc gcgaacgctt tatcaatgaa
     5821 gctgtcgttg agcctgtgtt gagtatataa ttttttactc gcgcgcttca cggattgctt
     5881 tttctaaatc gcgcgccatt tgtgcgagga catacatacg aaaatcgtct aaatttg
//
LOCUS       DAOMPD010000116         5929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5673_length_5929_cov_2.534559, whole genome shotgun sequence.
ACCESSION   DAOMPD010000116 DAOMPD010000000
VERSION     DAOMPD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5673_length_5929_cov_2.534559"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLY93_05345"
     CDS             complement(<1..201)
                     /locus_tag="PLY93_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRP68938.1"
                     /translation="MAEKYIVGDAKAKAHLTEIQNKKPSDITREDLKFLATRVRQNVV
                     EMIFRAASGHPGGPLGLADIYTY"
     gene            complement(223..1446)
                     /locus_tag="PLY93_05350"
     CDS             complement(223..1446)
                     /locus_tag="PLY93_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68939.1"
                     /translation="MQETTFSVSSAYVSRYDYNWLAHKLGIFKEGSGQRILPIPENFY
                     LNNHLTNTEYPLSGLDLLDNECNVRSDKVQLVRRYFKSLQVTGFENQKVDLRILSMRA
                     IPESVALRYSDATPQAVKCLSAQINALAEISFFKEPPSLVLPAFKSLRVNLSLNERNK
                     LLDDVGRNIATALTDIYKKNPTYKVRILLSNTIPVAQTQSLPFHELVGQFVGLTPDEL
                     QRVKTAAVQNAYVVPDANQRIEFALDTAVVWQLWQTEKINFFKDSSGEPLLYEDLVLS
                     LRKSLSESDNAGVIQNRHLKAIYASYARDNDEKKKWGESINLPYQRSSIERLMKLQVQ
                     MFLDVFDFSAVGLEGNTYIALEMPAREKFPLLHTLVRPLAHRWGLSEKKDGSDIAEIE
                     DSLSLLRSAVAQSGK"
     gene            1699..2754
                     /locus_tag="PLY93_05355"
     CDS             1699..2754
                     /locus_tag="PLY93_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRP68940.1"
                     /translation="MAQTSTVNLIGKDPVTPGWKNFHAIQRLVSFFIKDPRDIDLFTC
                     MVKLSILLPISAGVVWYFEEYRLYLGICHIALFLFYLGPYVLMLHNVSHRPPFHYKLR
                     FLNFWPQALLGIFFGMTPYTYFSHHLGMHHPENNLETDLSSTMRYRRDSFWGFLKYYI
                     IFLVGGVFTLGAYFIRHKRWSLFRKAVLGELFWFTVVGLLLYLNPSHFPREVTLFVLV
                     IPLLLSRFLLMAGNWTQHAFVDLSEPANPYLNSITFIDSPYNKRCFNDGYHIGHHVNM
                     NRHWLDMPADFLDNLHTYRDTRSIVFRKLDYFMIWFLLMTKQYKLLSRFFVDLSEKKL
                     SQDEIIALIKSRMEPLT"
     gene            2751..3779
                     /locus_tag="PLY93_05360"
     CDS             2751..3779
                     /locus_tag="PLY93_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68941.1"
                     /translation="MIFLAIDADTLRLPGFDASSVWYLTYALLAAVVFLLFLHWRQKK
                     DLAREALSNRFMQVLMKHELTHTQQTTIQNFFNSLNDNERAAILSSQKNLAYYLHAYL
                     ETHETINAIDRVQIYDKLLSELQSQTEIKSMADLCAGELCALILDGREYFATVAKTNT
                     TQALLSLHQHFSHAPQTKINLYAYRPHLGGFTLGGKILKANDHSVVIEHDGQIQFQSD
                     QHLMTTSPLTIHLERWPKDGIGIESSTTDAPSEGIDAFSGTTEKFSDRAFIVRLQENL
                     PDWVLKRQDLWQFQLALPEGALTCRVRMAHYNANNLYLLRPIDLTQADRRRLDKFIAE
                     NNPIHEHI"
     gene            complement(3784..>4720)
                     /locus_tag="PLY93_05365"
     CDS             complement(3784..>4720)
                     /locus_tag="PLY93_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP68942.1"
                     /translation="LLTGNAVLYKPSELALLSGNLMARYLHEAGVPTAAFQVVVGKGD
                     VGSLLIELPIDGVFFTGSVATGRKIHEAAAKKFMKVQLELGGKDPAYITDDVDIKAAA
                     EATADGAMYNTGQSCCSVERIYVHEKIYDDFVSHFLATVKTFKSGNPTDEATYIGALT
                     RGKPALDFLLAQVDDAVKKGARLACGGKALSTDTGYFLEPTVLLDVTHAMQVMREESF
                     GPVIGIMKVKDDAEAQKQMNDTTFGLTAAVYCRDTNRARKILEGVNAGSAYINCCDRV
                     SPRLPWSGRGLSGVGLTLSTYGIETFTRPKAWHVK"
     assembly_gap    4721..4735
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4736..5166)
                     /locus_tag="PLY93_05370"
     CDS             complement(<4736..5166)
                     /locus_tag="PLY93_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP68943.1"
                     /translation="MNTIDVVNPTSGKLITSLEVDNAQTIQQKYAAARVAQHAWNKTP
                     LQKRIEILRNFGKILEKNKEEAAETLTREVGKPISQSRNEISGTLGRLEFFVAAVEKT
                     TATQIVLDEKNPSMREEIEHEPLGVIANISAWNYPWFVGSNV"
     gene            5266..>5929
                     /locus_tag="PLY93_05375"
     CDS             5266..>5929
                     /locus_tag="PLY93_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRP68944.1"
                     /translation="MKILAQTLKIATGAILLLIVIVIINTYRKTSNQIKTTPLPPTAI
                     DEARVSKRLAEAVRLKTVSSRDDAELNADEFKALHTLLEKEFPLVHKTLKRETVNGLS
                     LLYTWQGEDHKAKPILLMAHQDVVPIAPGTEGDWKFSPFSGVVKDGAIWGRGAWDNKG
                     NLLAQLEAVEMLLAKGFKPSTTIYLAYGADEEVNGLRGASAISKLLASRGVELAFVLD
                     EGL"
BASE COUNT         1506 a         1500 c         1289 g         1619 t
ORIGIN      
        1 atacgtgtaa atatcggcaa gccccaacgg accgcccggg tgaccagaag cggcgcggaa
       61 aatcatctcg accacatttt gacgcacgcg tgtcgctaaa aattttaagt cctcgcgtgt
      121 aatatcgctt ggttttttgt tttgaatttc ggtgagatgt gctttcgctt tcgcatcgcc
      181 cacaatgtat ttttcagcca taaagtcttc cttaaactaa aattatttac ccgattgcgc
      241 caccgcgctt cgcaaaagcg acagtgaatc ttcaatttct gctatatccg agccgtcttt
      301 tttttcagaa agtccccagc gatgagcgag tgggcgcaca agcgtatgca acaacggaaa
      361 tttttcacgt gcgggcatct cgagcgcaat gtatgtgttt ccttctaaac ccaccgcaga
      421 aaaatcaaac acgtctaaaa acatttgcac ttgaagtttc atcaaccgct cgatgctcga
      481 tcgttggtaa ggtaagttga tactctcacc ccattttttc ttctcgtcgt tgtcgcgcgc
      541 atacgacgca taaattgctt ttaagtggcg attttgaatc acccctgcat tatcgctctc
      601 gctcaaggat tttcgtagcg acagcactaa atcttcgtaa agaagcggct cgcccgagga
      661 gtctttgaaa aaattaattt tttcggtttg ccaaagttgc caaaccacgg cggtatcgag
      721 ggcaaactca atgcgttggt ttgcatcggg gacgacgtac gcattttgta ccgccgctgt
      781 tttaacacgc tgcaactcgt cgggggttag gccgacaaac tgtcccacaa gctcgtggaa
      841 tggtaaactt tgcgtttgcg ccacggggat tgtgttactc aaaagaatac gtactttata
      901 agtgggattt tttttgtaaa tgtcggtgag cgctgtggca atattacgcc ccacatcgtc
      961 caaaagttta tttctttcat tcaacgagag attcacccgc aacgacttaa aagcaggcag
     1021 cacgaggctg ggcggctcct tgaaaaatga aatttcagcc agtgcattga tttgtgcgct
     1081 aaggcatttc actgcttgtg gcgttgcatc actgtaacgc agcgccaccg actcggggat
     1141 tgctcgcatc gacaaaattc tcaaatccac tttctgattt tcaaaccccg tcacctgtaa
     1201 cgacttaaaa taccggcgca ccagttgcac tttatcgctg cgcacattac attcgttatc
     1261 caaaagatca aggcccgaga gagggtattc cgtattggtt aaatggttat tcaaataaaa
     1321 attttcaggt ataggtaaaa tccgctgacc actcccttct ttaaaaatgc ccagtttgtg
     1381 tgccaaccag ttgtaatcgt aacgcgagac ataagcactc gaaacgctaa aggtcgtctc
     1441 ttgcatacgc cgcgtaaatt ttattggagt cgatggagcc ttaaccgaaa gattttcaaa
     1501 gctttctcga gtaaagaaag aaaccacaat tgaaattgct aaagcgctaa aaataagcgc
     1561 gaatagacta ctgaacgcct tacgcatagg ttacacctga gactaactac gcaaagcaaa
     1621 gcattttata cttgtttcct ttgccgccag ggtcggcttt tattcttttc acactgttac
     1681 aaaaagcatt ttttacgcat ggcacaaact tcaacggtaa acctcattgg gaaagatccc
     1741 gttactcccg gctggaaaaa ttttcatgcg atacaacgtc tcgtttcctt cttcattaaa
     1801 gatccgcgcg acatcgatct ttttacttgc atggtgaagc tcagcattct tttacccatc
     1861 tcggcgggtg tggtttggta ttttgaagaa taccggttgt atctagggat atgccacatt
     1921 gcgctctttt tgttttattt aggcccttac gtactcatgc tacacaacgt ctctcaccgg
     1981 ccgccgttcc attacaaact gcgcttttta aatttctggc cgcaagcgct cttgggtatt
     2041 ttttttggga tgacacctta tacttatttt tcacaccact tgggaatgca ccatcctgag
     2101 aataacctcg aaaccgattt gagctcgacg atgcgctacc gtcgcgacag tttttgggga
     2161 tttctcaagt attatattat ttttctcgtg ggtggcgtct ttacgttggg tgcatatttt
     2221 atccgccaca aacgatggtc gctttttcgt aaagcagtcc ttggggaatt attttggttt
     2281 accgtagtag gattgctcct gtatctcaat ccatcgcact ttccacgcga agttactctt
     2341 tttgtgctag ttatccccct ccttttgagt cgctttctcc tcatggcggg caactggacg
     2401 caacacgcgt tcgtggattt aagcgagcca gccaatccct acctcaacag catcacattc
     2461 atcgattcgc catacaacaa acgctgcttc aatgacggct accacatcgg ccaccacgtg
     2521 aatatgaacc ggcattggct cgacatgccc gctgattttt tagacaattt acacacttac
     2581 cgcgacacgc gttcgattgt ttttcgtaag ctcgattact tcatgatttg gtttttactc
     2641 atgacgaagc aatataaact cctcagtcgt ttttttgtcg acctgtcgga gaagaaattg
     2701 tctcaagatg aaattatcgc acttataaaa agccgcatgg aaccactcac atgatttttc
     2761 ttgccattga cgccgacaca ctacgcctgc ctggcttcga tgcgtcgtcg gtgtggtatt
     2821 taacgtacgc acttttggcg gcggtggtat ttctcctttt tttgcactgg cggcaaaaaa
     2881 aggatttggc acgcgaggcg ctttccaacc gttttatgca agtactcatg aagcatgagc
     2941 tgacgcatac ccaacaaacg acgatacaaa atttttttaa ctcgctcaac gataacgaac
     3001 gtgccgcaat actttcttcg caaaagaact tggcgtacta cttacacgcg tatcttgaaa
     3061 ctcacgaaac gataaatgct atcgaccgcg tacaaattta cgacaaactt ttatccgagc
     3121 tacaatcgca aaccgaaatt aaaagcatgg ccgatttatg cgccggtgaa ttgtgtgcat
     3181 taattttaga cgggcgtgaa tattttgcaa ccgttgcgaa aacaaatacg acacaagcgc
     3241 ttctttcgtt acatcaacat ttctcacacg cgccacaaac aaaaataaat ctttacgcat
     3301 accgccccca cctcggtggt tttacccttg gtggaaaaat tcttaaagcg aacgatcact
     3361 cggtagtcat cgagcacgac ggccaaattc aattccaaag cgatcaacat ctgatgacca
     3421 cgtctccttt gacgattcat ttggagcgtt ggcccaaaga cggcattggt attgaatcgt
     3481 ccaccacaga tgcacccagc gaagggattg acgctttttc aggcactaca gaaaaatttt
     3541 ctgacagggc atttattgtg cgccttcaag aaaatttacc cgactgggtt ttgaaacggc
     3601 aagatctgtg gcaatttcag cttgcccttc ccgaaggtgc gctcacttgc cgcgtacgga
     3661 tggctcacta caacgcgaac aatctttatt tgctccgccc gattgactta acgcaagcag
     3721 acagacgccg tctcgataaa ttcatcgcag agaataaccc catccatgaa catatttaac
     3781 cccctatttc acatgccacg ctttcgggcg ggtgaaagtt tcaataccat aggtagaaag
     3841 cgttaatccc acgccgctta accctctgcc cgaccacggc aagcgggggc tcacgcggtc
     3901 gcaacagttg atgtatgcac tgcccgcgtt cacgccttct aaaattttcc gtgcgcgatt
     3961 tgtgtcgcgg caatagacgg cggcggtgag accaaaagtg gtgtcgttca tttgcttttg
     4021 cgcctcggca tcgtctttga ctttcataat accaatcacc ggcccaaagc tttcctcgcg
     4081 catcacttgc atggcatgcg ttacatccaa gagcaccgtc ggctctaaaa aatatcccgt
     4141 gtctgtcgac agcgctttgc cgccgcaggc aaggcgtgcg ccttttttga ctgcatcgtc
     4201 cacttgcgcc aaaagaaaat ccaacgctgg tttaccccgt gtgagtgcac cgatgtacgt
     4261 cgcttcgtcg gtgggatttc ctgatttgaa cgtttttacg gttgcgagaa aatgactgac
     4321 gaagtcgtcg taaatttttt catgcacgta aatacgctca acgctacaac agctttgacc
     4381 ggtgttgtac atcgccccgt cagcggttgc ttcagcagcg gcttttatat cgacatcgtc
     4441 ggtgatgtac gcggggtctt tgccgcctaa ctcgagttgc actttcataa atttttttgc
     4501 ggctgcctcg tggattttcc tccccgtggc gaccgagccg gtgaaaaaca caccgtcgat
     4561 gggcagctcg atgagtaacg aacccacatc ccctttcccc accaccactt ggaacgctgc
     4621 agtaggcact cctgcctcgt gcagatagcg cgccataaga ttccccgaca aaagtgccaa
     4681 ttcggagggt ttatagagaa ccgcgttacc ggtcaaaagc nnnnnnnnnn nnnnnacatt
     4741 cgagccgaca aaccaaggat aattccacgc tgaaatgttc gcaatgacac ccaacggctc
     4801 gtgctcaatt tcttcgcgca tcgatggatt tttctcgtcg agcacgattt gagtagcagt
     4861 agtcttttcc accgccgcca caaaaaattc gagtcgaccg agagtaccgc tgatttcatt
     4921 acgcgattgg ctgatgggct tacccacctc gcgcgtcaac gtttcagctg cttcttcttt
     4981 atttttttcc aggattttac caaaatttct gagaatttca atccgctttt gtaaaggagt
     5041 tttattccat gcgtgctgtg caacacgagc ggcagcgtac ttttgttgga tagtctgtgc
     5101 gttatctact tctaaagaag taatgagttt gccactggtc ggatttacca catcgatggt
     5161 gttcatgcgt gctgaatgga gcgtcgattc agcgcgacaa tggatttata actttaggct
     5221 tgacgtgaac aaaattatat taagcgtacc aaaaaaagag agaacatgaa aatacttgct
     5281 caaactttga agatcgccac gggggcaatc ctactcctta tcgttatcgt tatcatcaat
     5341 acctaccgca aaacctcgaa ccaaattaaa accactcctc taccgcctac cgccatcgac
     5401 gaagcgcgcg tttcaaaacg actggctgaa gcggtgcgcc tgaagacagt ttcatcgcgc
     5461 gacgatgccg agctcaacgc ggatgaattt aaagcactgc acacgctttt ggaaaaagaa
     5521 tttcccttgg tacataaaac gctcaaacgc gaaaccgtca atggcctaag tcttctttat
     5581 acatggcaag gagaagacca caaagcgaaa ccaattctcc tcatggcgca ccaagacgtt
     5641 gtgcctattg ccccgggcac cgaaggtgac tggaaattct cccctttttc gggcgtggtg
     5701 aaagatggag ccatttgggg gcggggagct tgggataaca aagggaatct tttagcgcag
     5761 ctcgaagcag tggaaatgct tcttgctaaa ggctttaagc catcgacgac aatttacttg
     5821 gcttatggcg ccgacgaaga agtcaacgga ctacgcgggg cgtccgcgat ttccaagctg
     5881 ttagcgtcgc gcggcgttga gcttgctttc gtcttggacg aagggttgc
//
LOCUS       DAOMPD010000117         5904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5704_length_5904_cov_2.651222, whole genome shotgun sequence.
ACCESSION   DAOMPD010000117 DAOMPD010000000
VERSION     DAOMPD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5704_length_5904_cov_2.651222"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..>432
                     /locus_tag="PLY93_05380"
     CDS             132..>432
                     /locus_tag="PLY93_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68945.1"
                     /translation="MQTRIISLLLLSLLSLSFTTACQKADKAWVDRYKRSVRDDVDLF
                     LVPEKYALLEASWIKKTAVARYNKKQLEQALAEYGRSIDDLKKNPAYEYKEIGDEV"
     assembly_gap    433..442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <443..1834
                     /locus_tag="PLY93_05385"
     CDS             <443..1834
                     /locus_tag="PLY93_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRP68946.1"
                     /translation="RISPSTAYDYDTSIPLVFNGKWFQKQNNGTEVYQQHIAPTLAKV
                     MGISKPSGSTVDALPILTQEKSKPDIVVVTVIDQGGMGLINTHKSVAPRIQALMASSA
                     NFTNAHVGHLDAHTAVGHMAIGTGAFPIKSSVIGNTFFRMKKENGKNTLLQDEIYAGK
                     DESKVDTTELKAETLGDVLNRENKGNSVLASQSYALRASIGMGGHGALGLTQNAKEIA
                     ANSFIYWLNSSESRWITDTRYYSLPKVAAASDVLLAYLKYYPKGYEGYSIRDKKDAHK
                     NWGVIMATPAETQLEGELMRAVIREEILNKKKNHDGFTDLIYISFKSADAVGHQFGYH
                     SLQARETLAAIDGQIGAMQDFLTKEYGDNYVLVLTADHGCAPVSEISGGARLTIEEIY
                     AAVDALLPQDVAAKESLINFATVGQISLNHALMQQYNIPLEKIRTTILNLRPQGRTFF
                     KEILTRKDLGVDN"
     gene            1824..2768
                     /locus_tag="PLY93_05390"
     CDS             1824..2768
                     /locus_tag="PLY93_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRP68947.1"
                     /translation="MTIDTRLHYKNKFVAGLVFTILGAAGFSAKAILAKLCYRYGITG
                     FATLTLRMLFALPLYAIIFTYYFRRETKEIQTHFKRDIFVVSLVGLFGYYLASWFDFV
                     GLEYISASFERLLLFIYPTLVLFISLVWLKKRAGRREIAALVITYIGIGVAYHNEAAS
                     LGEKAHIGAVYIVLAAITYAIYLVATEKLSKRYSPMVFTSFAILVSAFAILIHSWIMQ
                     EKIWGFPREVYYYTVAMAIFCTVIPSLLIALGIAYIGSNRAAILSALGPVLTIYLAYL
                     VLGEDVNLIQTIGTVFVIGGVALLTIKPKQEKHAESLS"
     gene            2749..3699
                     /locus_tag="PLY93_05395"
     CDS             2749..3699
                     /locus_tag="PLY93_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRP68948.1"
                     /translation="MLNLLVNARQGDKFSIAKLISLFESSSSGAVDTCVALDKELGET
                     PRGLLVGLTGAPGTGKSTLIQHLGNVLLEKKPTWRLAVVAVDPSSPVSGGALLGDRTR
                     VIFHDTERTYFRSQASDLDLGGIGRNTFRVCRFLRHFFDVVLVESVGVGQSEIEIARL
                     ADVTLLALQPFSGDQIQFMKSGVMEMPQAFIITKCDAEDLARKSYHQLRASLNFLGRI
                     EALPGEERHIPNIFQTSSLNKTGFEELADFLINSKPLSVTEEAKREEYFLKKELRYFY
                     GEFGVEVMQKEITRTASLLFEERRQTLLNALDARWKKYAT"
     gene            3689..4309
                     /locus_tag="PLY93_05400"
     CDS             3689..4309
                     /locus_tag="PLY93_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP68949.1"
                     /translation="MPPKKNKTLFLTYQNLSRRYGAEWALKEASGELFENEIVALEGE
                     NGSGKSTLLLTLAGILRPHKGAIAFHNTSAHLVAHHPMAYIALSIERNLALAHAIDKN
                     ARHSLDATMEFWQIAPLKAKALSSLSRGQMQRFLLARSMLTSSRVLLLDEPFTGLDVH
                     GEKLLEEFIRSEAARGVAVLFSDHDHARAQKLATRTIRMKAGRCIS"
     gene            4306..4965
                     /locus_tag="PLY93_05405"
     CDS             4306..4965
                     /locus_tag="PLY93_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68950.1"
                     /translation="MKAFWELLKNEARLYAAGKGLSFALVLSLSLLALIRFGMPVDGI
                     EAQGFEVFPIVVYCIAVLQLLLMATQWESDGYAYRYYSMQRFSLSSLFLAKSIVSYFV
                     QIPIWFFCFTGYFLFFPADVPPISILIPLLATTLYLGLALAPAGQMIAALSQHSAQKN
                     FLALALFLPICLPVLIAASGRVTALLQRLDTVRYDALLFAAALIFWGVGNLLFAYLFE
                     E"
     gene            complement(4996..5505)
                     /locus_tag="PLY93_05410"
     CDS             complement(4996..5505)
                     /locus_tag="PLY93_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68951.1"
                     /translation="MTSFFTAAHLKLSILLAVLGTLVALIFTILSDSGITALIVRPIV
                     SGLFMFILGSVVFAVLEKKVPEALDVIHPPLETPGEGEALDALADTENSSLEASDEAL
                     THDSSSEADGIDSYTSSDISTSAGSSIPRKTGVQISKDEIVVNGVKFKNQPEVMAETI
                     KQLMDDDKE"
     gene            complement(5518..>5904)
                     /locus_tag="PLY93_05415"
     CDS             complement(5518..>5904)
                     /locus_tag="PLY93_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRP68952.1"
                     /translation="VVTTPEPTAITDAYGIIKSIVLIAPDKQIKLLVNRATSSLEGKK
                     VAERVINISNQFLNVRVENLGFIYDDDAVSKSIRRQKPFYLLFPNSKAAACLDIVTAR
                     ILEQELPKRNEAGIGAFFTKIFAPSV"
BASE COUNT         1496 a         1407 c         1416 g         1575 t
ORIGIN      
        1 tgcggcgtag catttgtcag gtatgtccgt gggttagaca agaacttagt aagaatatgc
       61 aacccacagg ccttagaatc ataaacattt ccacatcttg acgcactcgc tctaaactga
      121 gaacataccc tatgcaaact cgaattatct ctctcctttt actctcttta ctctctctct
      181 ctttcaccac cgcttgccaa aaggccgaca aagcgtgggt cgatcgttac aagcgttctg
      241 tgcgcgacga tgtggattta tttttagtcc ccgaaaaata cgcgcttctc gaggccagtt
      301 ggattaaaaa aaccgctgtc gcgcgctaca ataaaaagca actcgaacaa gcgctcgctg
      361 agtatgggcg ttccatcgac gacctcaaaa aaaatcctgc gtatgaatat aaagaaatcg
      421 gcgatgaagt cannnnnnnn nncgtatttc cccctcgact gcttatgatt acgacacgtc
      481 catcccactt gtctttaatg gaaagtggtt tcaaaaacaa aacaacggca ctgaagttta
      541 tcaacagcac attgcgccta cactcgctaa agtgatggga atttctaaac catcgggttc
      601 gacagtggac gcattaccaa ttttaacgca agaaaaatct aagcccgaca ttgtcgtcgt
      661 cactgtgatt gaccagggcg gtatgggact catcaacacg cacaaaagcg tggcgccgcg
      721 catccaagcg ctcatggctt cttcggcaaa tttcactaac gcgcacgtcg gccatctcga
      781 tgcacacacc gcagtggggc acatggccat cggcacaggg gctttcccga ttaaaagttc
      841 ggtgattggc aatacgtttt tccgcatgaa aaaagagaac ggtaaaaaca cacttctcca
      901 agatgaaatt tacgcgggta aagatgaatc aaaagtcgat accaccgaac ttaaagccga
      961 aacattgggc gatgtgttga accgagagaa caaaggcaat agcgtgcttg catcgcagtc
     1021 gtatgcgcta cgtgcatcga ttgggatggg cggccacggc gcactcggac tcacccagaa
     1081 cgccaaggaa attgcagcga acagttttat ctactggctc aattcgtcag aatctcgttg
     1141 gatcaccgac acccgttact attcactgcc caaagtagcg gcagcgagcg atgtattgct
     1201 cgcgtacttg aagtattacc ccaaaggcta cgaagggtat tctatccgcg ataaaaaaga
     1261 tgcacacaaa aactggggcg tgataatggc gacccctgct gaaacccaac tggaaggcga
     1321 actcatgcgc gctgtcatcc gtgaagaaat tttgaataaa aagaagaacc atgatggctt
     1381 taccgacttg atctatatat ctttcaaatc cgccgacgcg gtgggacacc agtttggcta
     1441 ccactcgcta caagcgcgcg aaactcttgc ggccatcgac gggcaaatcg gagcgatgca
     1501 agatttcctc acgaaagagt acggcgataa ttatgtgttg gtgctcaccg ccgaccacgg
     1561 ttgtgcgccg gtgagtgaaa tttccggtgg cgcacggtta acgattgaag aaatttacgc
     1621 agcggtcgac gcgcttctgc cgcaagatgt ggcggcgaaa gaatcactca tcaattttgc
     1681 gacagtgggg caaatttcgc tcaaccacgc gctcatgcaa caatacaaca tcccactcga
     1741 aaaaatacgc acaacaatac tgaatttgcg gcctcaaggc cgtactttct ttaaagagat
     1801 tctcacccgt aaggatctcg gcgttgacaa ttgacacacg gctgcattac aaaaacaaat
     1861 ttgtcgcggg tcttgtcttt accattttag gtgcggcagg cttttcggca aaggcgattc
     1921 ttgccaaact gtgttatcgc tatggcatca cgggttttgc cacgttgacg ctccgcatgc
     1981 tctttgcgtt gccgctttac gccattattt ttacgtatta tttccgccgc gaaacgaagg
     2041 aaatccaaac acatttcaag cgcgatattt ttgtggtttc tttagtcggc ctctttgggt
     2101 attatttggc tagttggttt gattttgtag gtttggaata tatctccgcc agttttgaaa
     2161 gattgttgct ctttatctac cccactcttg tgctttttat ttcactcgtg tggctcaaaa
     2221 agcgcgcggg gcgtcgagaa attgccgcgc tcgtgattac atatatcggc attggtgttg
     2281 cataccacaa cgaagcggcg tcgttgggag agaaagcgca tatcggcgct gtgtatattg
     2341 tgctcgccgc tattacgtac gcgatttatc ttgtggcaac cgaaaaactc tcgaagcgct
     2401 attcacccat ggtctttacc agctttgcca ttttggtttc ggcgtttgct attttaatcc
     2461 acagttggat tatgcaagaa aaaatatggg gatttccacg cgaggtgtac tattacaccg
     2521 tggcgatggc tattttttgt acagtgatac cctcgctttt gattgcattg ggcatcgctt
     2581 atatcggctc aaaccgtgcg gcaattttga gcgcgctggg gccggtatta acaatttact
     2641 tagcatatct tgtgctgggc gaagatgtga atttgataca aacaataggc actgtgtttg
     2701 tgattggcgg agtggcgctt ttaacgataa agcctaaaca agaaaagcat gctgaatctc
     2761 ttagttaacg cacgacaagg cgataagttt tcgatcgcaa aactcatttc cctttttgaa
     2821 tcatcgagta gcggcgccgt cgacacttgt gtcgctctcg ataaagaatt gggagaaact
     2881 ccgcgtggac ttttagtagg actcaccggc gcgcccggca ctggtaaatc gacactcatc
     2941 caacacttgg ggaatgtgct tctggaaaaa aagccaacgt ggaggttggc cgttgtcgcc
     3001 gttgatccat cgagccctgt ttccggtggt gcgcttttgg gcgaccgcac acgcgtaatt
     3061 tttcacgaca ccgagcgcac ttacttccgc tcgcaagcgt ctgatttaga tttaggtggt
     3121 ataggtcgca acacgtttcg ggtgtgccgt ttcttgcggc atttttttga cgtggtgctc
     3181 gtcgagtcgg tgggtgtggg gcaaagcgaa attgaaatcg cacgccttgc cgatgtgacg
     3241 ctcttggcgc tgcaaccttt tagcggcgat caaattcaat tcatgaaatc gggcgtgatg
     3301 gaaatgccgc aagcgtttat catcactaaa tgcgatgcgg aggatttagc gcgcaaaagc
     3361 taccatcaac tccgcgcgag tctcaatttt ttagggcgca tcgaagcatt gccgggggaa
     3421 gagcgtcaca tccccaacat ttttcaaacc agctcgttga acaaaaccgg ttttgaagaa
     3481 ctggctgatt ttctcattaa ctcaaaacca ctttctgtaa cggaggaagc taaacgcgaa
     3541 gaatattttc tcaaaaaaga gttacggtat ttttatggcg aatttggtgt cgaagtgatg
     3601 caaaaagaaa tcacgcgcac cgcatcgctt ttattcgaag agcgccgcca aactctactc
     3661 aatgcgctcg atgcccgctg gaaaaaatat gccacctaag aaaaacaaga cactttttct
     3721 aacgtaccag aatctttccc gccgctacgg cgctgagtgg gcgctcaaag aagcaagcgg
     3781 cgaacttttt gaaaatgaaa tcgtcgctct cgaaggagaa aatggttcgg gtaaatcaac
     3841 gcttctttta acgctcgcag gcattttacg tccgcacaaa ggtgcgatcg cgtttcacaa
     3901 tacatccgcg catttagtgg cgcaccatcc catggcgtat atcgcgcttt cgatagagcg
     3961 caatttagcg cttgcgcacg ccatcgataa aaatgcgcgc cacagcctcg atgcaacgat
     4021 ggagttttgg caaattgcgc ctctcaaagc aaaagcgctc tcgtctctct cgcgaggaca
     4081 aatgcagcgc ttcttacttg cgcgttcgat gttgacgagt agccgtgtgt tattgctcga
     4141 cgagcctttc acaggactcg atgtgcacgg cgaaaaatta ttagaagaat ttatacgtag
     4201 cgaagccgcg cgtggtgtag cggttttatt ttcagaccac gaccacgcac gcgcccaaaa
     4261 attagcaacg cggacaatcc gcatgaaagc aggcaggtgc atttcatgaa agcgttttgg
     4321 gagttactta aaaacgaagc gcggctttat gctgcgggta aagggctttc atttgcttta
     4381 gtgttgtcgc tttcgctttt ggcgctcatc cgctttggca tgccggtcga cggtatcgaa
     4441 gcgcaaggct ttgaagtctt tcctatcgtt gtttattgca ttgcagtttt acaacttttg
     4501 ctcatggcga cccaatggga aagcgacggc tacgcatacc gttattattc aatgcagcgt
     4561 ttttcactca gtagcctttt cttagcgaaa agtattgttt cctattttgt ccaaataccc
     4621 atttggtttt tttgttttac cggttacttt cttttttttc cggcagatgt ccctcctatc
     4681 tcaattttaa ttccactatt ggcaacgacg ctctacctgg ggttggcatt ggcgccggcg
     4741 gggcaaatga ttgcagcgct cagccaacat agtgcacaaa agaatttttt ggcgttggct
     4801 cttttccttc ccatttgttt gccggtgctc attgcagcgt ctgggcgggt cacagcgctt
     4861 ttacagcgac tcgataccgt gcgctatgat gcgctccttt ttgcagcggc tcttattttc
     4921 tggggagtcg gtaatctact ttttgcttat ctctttgagg aataaagatt aacgactcat
     4981 ccaaataaac tttcattatt ctttgtcgtc gtccatcagt tgcttaattg tttctgccat
     5041 gacttcgggt tggttcttaa attttacgcc gttgacgaca atttcatctt tactaatctg
     5101 tacgcctgtt ttacgaggaa tgctcgaacc cgccgaggtg gaaatatctg aactcgtgta
     5161 cgagtcgatg ccgtccgctt cagaagaaga gtcgtgtgta agcgcctcgt ccgaagcctc
     5221 aagcgaagag ttctccgtat ctgcaagcgc atcgagtgct tccccttcgc caggagtctc
     5281 aagcggcgga tggatgacat cgagagcctc ggggactttt ttttcaagca cagcaaaaac
     5341 gacgctgccc aaaataaaca tgaaaagccc gctgacaatc ggccgcacga tgagcgcagt
     5401 gatgccgcta tcggaaagaa tagtaaaaat gagagcgaca agagtgccga gtactgcaag
     5461 caagatgctg agtttaagat gagccgctgt aaaaaacgat gtcatctctt agcggattta
     5521 caccgacggc gcaaagattt ttgtgaaaaa agcgccaata ccggcttcgt tacgtttggg
     5581 taattcctgt tccaagatac gagcggtgac aatgtcgagg cacgctgctg cttttgaatt
     5641 ggggaagagt aaataaaacg gtttttgacg gcggatggat tttgaaacag catcgtcgtc
     5701 gtagatgaaa cctaaatttt caacgcgcac gtttaagaat tgattcgata tgttgatgac
     5761 ccgctcggca actttttttc cttcgagaga cgaagtggcg cgatttacca acagtttgat
     5821 ttgtttgtcg ggcgcgatga gtacaatcga tttaatgatg ccgtacgcat cggtaatcgc
     5881 cgtgggttcg ggcgttgtca ccac
//
LOCUS       DAOMPD010000118         5879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5739_length_5879_cov_3.251202, whole genome shotgun sequence.
ACCESSION   DAOMPD010000118 DAOMPD010000000
VERSION     DAOMPD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5739_length_5879_cov_3.251202"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..765)
                     /locus_tag="PLY93_05420"
     CDS             complement(217..765)
                     /locus_tag="PLY93_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68953.1"
                     /translation="MEELVKCRKQPQFLEKRPVKIANLIEVHLSVKQWARIVEIAAHR
                     GKTFSLITRYCVLRLARKENLRWTPIAMRAHTKMKKEKQLVNEFHRHVVCLYGEDEML
                     IRLAALRLKITMSAFIRLSLQLFLNRLAMENHSYRFITDETLKWQAIRFIEKSTTRTQ
                     NQLERPSSHRLKWFTFALSSFW"
     gene            complement(986..1435)
                     /locus_tag="PLY93_05425"
     CDS             complement(986..1435)
                     /locus_tag="PLY93_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68954.1"
                     /translation="MIKKIFFALLLGTSTIFADPVECMDEATINQIQPKIKNGAILYR
                     DCYFCSEPAFEKIQIIKSEIRKCHLAGSENERAIYTSGVVVERFQMPTCNKMEEKKLT
                     FKYITDELLVLNYSYINDGGNNLFKNIVDLLGGKSFHSCTSFDAGSQ"
     gene            complement(1579..2685)
                     /locus_tag="PLY93_05430"
     CDS             complement(1579..2685)
                     /locus_tag="PLY93_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68955.1"
                     /translation="MSLKIVALKNKKSDVKKFVNFMKRVYKSYPHFVPPIYNDVVKFI
                     MKGPFNKIGEKQLFLAYRGNKIVARLSVHRSFAHNDHYKVNQGFFGFFEAFDDQEAVN
                     AIFEEGKKWLKERGCTSVMGPMNFAIYDEIGLLIDAFDSDPVLMCTYNPPYYLKLLEN
                     AGFTKEIDWYAFLKDTTIPNFMRLINNRVVKQKGLVIREVNKKKIVEEAEAVKHIFNQ
                     AWAENWGNVPFTDDQWHHLVKDLKMIINEKLAFITELDGVPIAFSISLVDANQAVKKA
                     KGRLFPLGIVKLLLEMKKIHRVRTTIMGVLKEHRNHGIEIAMIHKTMENGVPMGYSEA
                     DCSLIVETNTAMISVLEKIGCTRYKTFRLLKSAM"
     gene            complement(3024..>5879)
                     /locus_tag="PLY93_05435"
     CDS             complement(3024..>5879)
                     /locus_tag="PLY93_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRP68956.1"
                     /translation="QVIFEDGVDDYWARTRVLERLNDAEIPEGVSIEVAPPWGPVSEI
                     YRYVLVSNQDHTPMDLRTIQNWTVIPRLMRAGGVVDVVNFGGLQKQYHVVTTPQKLAA
                     YRLTIQQVIAAIQANNVNTGGNIIRRGEQGFIVRAVGQIRRADDIRNIVVNQQAGVPV
                     FIWQIATVEEHPLIPLGVLGYALKEKNKPIEISDSAVQGLVVMRRDAAVDDTLKNVKA
                     AIYELNTNGALPEGVQIKETYDRGDLVNYTIKTVGKTLFEGISIVTLILIFFIGSVRS
                     ALVVATTIPISLLFAFILMRITGIHANLLSLGAIDFGIIVDGAVVMVENLMRNYRERG
                     NDEESLTALTFRSAREVGKEIFFSITIIVLAYLPIFTFERVEGKLFSPMAFTLAYAIL
                     GSMLLALTAIPALMTYLYRKAHKEKIIDKIEWHNPLYAWIEKKYAVIVAYTLPRSKKV
                     LGSAFFILVFSLGIGYWRIGTEFLPELDEGAINIRAIFPIGISLDAAKEYTPLIRDTI
                     AANDEVKVVLTQLGRNDDGSDPYGPNRLEVLVGLKDYSEWKSGSKKPDLLKKIKRQLT
                     EKIPGVNFLFSQPILDNVTEAVTGSVGDLAILINGSDLTRMREIAKRILDVLQKIPGS
                     SEAGIEQEGNQAQLNIEINREKAARFGINVADIQSMVEAAIGARVIGKLYDGPMRFDI
                     VVRYAAPYRNSVKAVEDLLINAPGGARIPLSQLADINFKDGPTMIQRQDGKRQISVRT
                     NIRGRDQGGFAKEAQIKVKALEIPPEFRIAWGGQYENLARAGKRLAVVTPLTIMVIFI
                     LLFSLYRNVRDVWAVMACLPLALVGGILALALRGYQFNVSSGVGFISLFGISVMTGVL
                     YVSRVRQLMRENTHLLLKTAVEEAAVTQLRPRLMTILLALMGLVPAMLATGIGSDVQR
                     PLATVIVGGMAAQLVLTLIVLPSIFLLLNSKHSTLRV"
BASE COUNT         1600 a         1345 c         1292 g         1642 t
ORIGIN      
        1 caaacgtaat cgccaaaagc gagcttacgg ccgccgaggt cgcgactgcg atgccataag
       61 aagaaaggta ggcaatcgtg cgaaaaagaa cacgaacaat actcgacatt atgtatactt
      121 tattttaaga aagagagggt aaaggccaaa ataaagataa gtggtgccca agagcctcat
      181 gtgggtaggc cggaactcga tgtggtaaat tttatgttac caaaaggagg atagcgcaaa
      241 cgtaaaccat ttgaggcgat gagaactggg ccgttcaagt tggttttggg tgcgtgtggt
      301 ggatttttct ataaaacgga tagcttgcca cttaagagtt tcatccgtta taaatcgata
      361 gctatgattt tccatagcta aacgatttaa aaataattgt agcgacaatc gaataaaagc
      421 agacattgta atctttaaac gcaatgctgc cagtcggatc aacatctcat cttctccata
      481 caaacaaacc acatgacgat gaaattcatt gacaagttgt ttttcttttt tcatttttgt
      541 gtgggctctc attgctatcg gagtccatcg caagttctct ttacgcgcta gtcgtaacac
      601 acaatagcgg gtgattagtg aaaacgtttt gccgcgatga gctgcaatct ccacaatccg
      661 cgcccactgt tttaccgata aatgtacctc aattaaattt gcaattttta caggacgttt
      721 ctccaaaaat tgtggttgtt ttctgcattt cactaattcc tccataaggg gaagatgtct
      781 tgtttagact ttcatcaccc tctatagatt aaataccaga aaacgtctaa attttctcac
      841 ttttgagaaa aaaaatgcga attttgtaga aaaatatcaa atttttggat ttttgaggta
      901 gtccggtcat gaatagctat ggaaattcat agctatgcca agttttgaat atagctatgg
      961 aaatccaaag ctaaagtcgt ccgttttact gcgaacctgc atcaaaactt gtgcaagagt
     1021 ggaatgattt cccacccaaa agatccacaa tgtttttgaa gagattatta ccaccatcat
     1081 tgatatagga ataattcagc accagcaatt catcggtgat atacttaaaa gttaattttt
     1141 tttcttccat cttgttgcaa gtgggcattt gaaatcgttc aacaacaact ccactagtgt
     1201 aaatggcgcg ttcgttttcg cttcctgcta aatggcactt gcggatttcg cttttgatga
     1261 tttgaatttt ttcaaatgcg ggctctgagc aaaaatagca atcccgataa agaatggcac
     1321 cgttttttat cttcggttgt atttggttta tggtggcttc atccatacac tcgactgggt
     1381 cggcaaaaat tgtagatgta cctaaaagca aggcaaaaaa aatcttttta atcataggac
     1441 agtcgcatag aaatttgaag tctatgcgcc aactcttaac tttcattttg cgatcgattg
     1501 gggcgcgcga tatatcaggt aggggatgtt gatggcaaaa gcaagtgtct tcaaattgcg
     1561 aggcttttca acactctgct acatcgcact cttcaacaac cgaaatgttt tgtaacgagt
     1621 acatccgatt ttttcgagaa ccgaaatcat cgccgtgttt gtctcgacaa tgagcgagca
     1681 atccgcctcg ctatacccca tgggtacgcc gttttccatc gttttgtgga tcatggcaat
     1741 ttcaatgccg tggttgcggt gctctttcaa aacgcccatg atggtggtac gcacgcggtg
     1801 tatttttttc atttcgagaa gaagcttcac aatccccaac ggaaaaagcc tgcctttggc
     1861 tttcttcacc gcttggttgg cgtcgacaag gctgatggaa aatgcaatcg gtacgccgtc
     1921 gagttcggtg atgaacgcca atttctcgtt gatgatcatc ttaagatctt ttacaaggtg
     1981 gtgccattga tcgtcggtga aaggcacgtt gccccagttt tccgcccacg cttggttgaa
     2041 aatatgtttc accgcttcgg cttcttcgac gattttcttt ttattcactt cgcggatgac
     2101 aagtcccttt tgtttcacga cgcggttgtt gatgagccgc atgaaattgg ggatggttgt
     2161 gtctttcaag aatgcgtacc aatcgatttc tttggtgaag cctgcgtttt caagcagttt
     2221 taaatagtac ggtgggttat acgtacacat gagcacaggg tcagaatcga atgcgtcgat
     2281 gagtaagccg atttcatcgt aaatcgcaaa gttcatcggc cccatcaccg aagtacatcc
     2341 gcgctctttg agccattttt tcccttcttc aaaaatcgcg ttcaccgctt cttggtcgtc
     2401 aaacgcctca aaaaaaccaa aaaaaccttg gttgactttg tagtggtcgt tgtgagcaaa
     2461 actgcggtgg accgaaagcc gcgcgacaat tttattgcca cggtatgcca agaataattg
     2521 cttctcgccg attttattga acgggccttt catgataaat ttaacgacat cgttgtaaat
     2581 cggcggcaca aaatgcggat agcttttgta gacgcgcttc ataaaattga cgaatttctt
     2641 tacgtcgctt tttttatttt taagggcaac tattttgagg gacatacgct caaacagagt
     2701 ataaagatgc accgccaaga ttttttattt tttcttgtga gttaaacact atcgactatg
     2761 gttccatggc cgaaaatcga tagcctgcac acatagagga gagtttttct ttaaattttc
     2821 cctgattttg accactccac atttgcggtt catagttact ccttaatgcg acataatctt
     2881 aaaaaatcgg catagttatc cccaaatgcg acataatcgt aaaaaattga catagttatc
     2941 ccccaatgtg acataatcgt aaaaaatcga catagttatc ccttaatgtg acataatcca
     3001 acttgaaaag tgctgcatca gcattacacg cgcagggttg aatgcttact atttagcaat
     3061 aaaaaaatag aaggcagtac tatcaacgtc aataccagtt gtgccgccat tccaccaaca
     3121 atcaccgttg ccaaagggcg ctgtacgtct gaaccaatac cggtggcgag catcgcgggt
     3181 acaagcccca tgagcgctaa aaggattgtc atcaaacgag gacgtaattg tgtcacagca
     3241 gcttcttcga cagcggtttt taaaaggagg tgtgtgtttt cacgcatgag ttgacgtacg
     3301 cgcgaaacgt agagaacgcc ggtcatcacc gatataccaa aaagtgaaat aaagcctact
     3361 cccgaagaaa cattgaactg gtacccccgc aatgccaacg caagtatgcc accaacgagt
     3421 gcgagcggca aacatgccat aacagcccac acatcgcgca cgttgcggta aagagaaaaa
     3481 agcaaaataa aaataaccat gattgtcagt ggagtgacta ccgctaatcg tttaccggcg
     3541 cgcgcaagat tctcgtattg cccaccccaa gcgatacgaa actctggtgg gatttccaac
     3601 gccttcactt taatttgcgc ttctttggcg aacccacctt gatctcggcc acgaatattc
     3661 gtgcggactg aaatctgccg tttgccgtct tgccgttgaa tcatcgttgg cccatctttg
     3721 aaattgatgt ctgccagttg cgagagtgga atgcgcgcac caccgggcgc gttgatgagc
     3781 aaatcttcca ccgctttcac agaattgcga tacggtgcgg cgtaccgcac cacaatatca
     3841 aaacgcattg gcccgtcata gagcttacca atgacgcgtg caccgatagc cgcttcgacc
     3901 atcgactgaa tgtcggcgac gttgatgcca aagcgtgccg ccttctcgcg gttgatttca
     3961 atgttgagct gcgcttgatt accttcttgt tctatccccg cttcgctcga ccctggaatt
     4021 ttttgcagta cgtcgagaat tcttttcgca atttcgcgca tccgtgttaa atccgaacca
     4081 ttgattaaaa tcgccaagtc accgacgcta ccggttacag cctcggtgac attgtctaaa
     4141 attggctgtg aaaacaaaaa atttacccca gggatttttt ccgtgagctg cctttttatt
     4201 ttcttcaata aatcaggctt tttagaaccg cttttccatt cggagtaatc ttttaatccc
     4261 actaacactt caagacgatt ggggccataa ggatcgctgc cgtcgtcgtt gcgtcccagt
     4321 tgcgtgagca ccactttcac ttcgtcgttt gcggcaatag tgtcgcgtat cagtggtgtg
     4381 tactcttttg cagcgtcgag ggaaatacca atagggaaaa ttgcccgtat gttgattgct
     4441 ccttcgtcaa gctccggcaa aaattcggta ccgatgcgcc agtaaccgat gccgagcgaa
     4501 aaaactaaaa taaagaatgc actccccaaa actttttttg agcgcggcaa tgtataagcg
     4561 acgatgactg cgtatttttt ttctatccac gcatagagag gattgtgcca ttcaatttta
     4621 tcaataattt tttctttgtg cgctttacga taaaggtatg tcatgagcgc gggtattgca
     4681 gtgagcgcta aaagcattga acctaaaatt gcatacgcga gcgtaaacgc catcggtgaa
     4741 aataatttac cctcgacgcg ctcaaaagtg aaaataggta agtacgccaa aacgataatc
     4801 gtgatagaga aaaatatttc tttccccact tcgcgcgccg aacgaaaagt gagggcagtc
     4861 aaactttctt cgtcgttgcc gcgttcgcgg tagttgcgca ttaaattttc taccatcacc
     4921 accgcaccgt cgacgataat tccaaagtcg atggcgccta acgaaagtaa atttgcatgg
     4981 atgcccgtga tgcgcatgag aataaaagca aagaggagtg aaattggaat cgtcgtcgct
     5041 acgaccaacg ccgagcgcac gctaccgatg aagaaaatta aaatgagtgt aacgatggat
     5101 ataccttcga acaaagtttt accgacggtc tttatcgtgt aattcactaa gtcgccgcgg
     5161 tcgtacgttt ctttaatttg cacaccttcg gggagcgcgc cgtttgtgtt taattcgtag
     5221 attgccgctt ttacattttt gagagtgtcg tcgacggcgg cgtcacgacg catgacgacg
     5281 agcccctgca ccgccgaatc ggaaatttct atgggcttat ttttttcttt gagtgcatag
     5341 cccaaaactc ccaaaggaat caacgggtgt tcttcaacgg tggcaatttg ccaaataaaa
     5401 acgggcactc ccgcttgttg attgacgacg atgttgcgga tgtcgtctgc acggcggatt
     5461 tgccccaccg cacgcacaat aaatccctgc tcgccgcgtc gaatgatgtt gccgccggtg
     5521 ttgacgttat tggcttgaat cgcagcaatc acttgttgga tggtcagtcg atacgcagca
     5581 agtttctgcg gggtggtaac gacgtggtat tgtttctgca aaccgccgaa attgaccacg
     5641 tcgacgactc caccggcgcg catcaacctg ggtatgactg tccagttttg gatggtgcgt
     5701 aaatccatcg gcgtgtggtc ctggtttgag actaaaacat aacggtagat ttcactcacc
     5761 ggcccccacg gcggcgcaac ttcgatagaa acaccctcgg gaatttccgc atcgttgagc
     5821 cgctctaaaa ctcgagtgcg cgcccagtaa tcgtcgacgc cgtcctcaaa aataacttg
//
LOCUS       DAOMPD010000119         5870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5752_length_5870_cov_2.926397, whole genome shotgun sequence.
ACCESSION   DAOMPD010000119 DAOMPD010000000
VERSION     DAOMPD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5752_length_5870_cov_2.926397"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLY93_05440"
     CDS             complement(<1..401)
                     /locus_tag="PLY93_05440"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HRP68957.1"
                     /translation="MLNADSHIADSTDPLLLSSDSRLWLTLRDKLKKKLPSQVFQIFF
                     QDIKAEIKGNDLILTAPSHEIAKHVQAQYARAIQDDAISLGFQGKIITTHTKQEKTPQ
                     PAATLTTPKAKPSEAAFPNRIDLNPHYTFDR"
     gene            complement(570..1004)
                     /locus_tag="PLY93_05445"
     CDS             complement(570..1004)
                     /locus_tag="PLY93_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRP68958.1"
                     /translation="MLDFNIFITILRHVFRDFIGVQELISEEMITLPEKFQSVTAKME
                     LTGSSPATLWLNMTELGIKIAMKKLAIPQSDEDRMLMDTVGELLNIIVGTAQRQSAVR
                     YDFSLPTALKGENYPLRFNENSRRSAHRFIFEQFEALLILEE"
     gene            complement(991..1374)
                     /locus_tag="PLY93_05450"
     CDS             complement(991..1374)
                     /locus_tag="PLY93_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP68959.1"
                     /translation="MNLLIVDDSELMRTIVEQTVRENLGHALDKVQFFHAANGADAME
                     IMQTNTVALIFLDWNMPVLDGIDFVREVRGSGNKVPIIMITSVTDSEKILQAAAVGVN
                     SYIEKPVRGPKLWNQIEMFFKNAGF"
     gene            complement(1378..3711)
                     /locus_tag="PLY93_05455"
     CDS             complement(1378..3711)
                     /locus_tag="PLY93_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRP68960.1"
                     /translation="MKRRFFNFRVRLNTALFFVAFSATITGLTYYYKVASEQVWAQMK
                     SRVKDFGKIGVTTLSSADILFLENLDIDINAVPRALPPEPQSGARLGALSQNEKMAIS
                     RSTSFQIIMQKLRRIRYASGKKPTYDSTIAASALLDQNVQIHRIWIAGVHMHAYAPDL
                     LRVLVADEIQEIDRNNNHRIDPEESIYHIGDVFNGHGQNSIASALKGETTVSNGYRTE
                     SLGVYISGYTPIINSRGKIIALLVIDFSAASEFDALFGLKITGYYIVVAVFLFSILAA
                     SITSRFLLKPLDAMQKAAAHFGQRDFSIRLEEDTNDEFGDLAYAMNLMAHELGEYSQN
                     LEERIFTRTQEISGILDSLEQGILTVDSRGFIQPEHSQRALAIFGLKEIAHKKLSSLF
                     EDKKLQASLEQYLSLFFGENVVSPQMLEQANPLTEIHYTNFLHEKKFLRFAFRPLETS
                     TRTPLKRILVSIVDETDEFTLKAKIEASENQKRFEFDILIQWMQIPPQILENFLVQQK
                     EIIRRGKEIIARFDNVSNATFVDFAQRVHALKGNALQLDFASLAETLHLLEEQVLAKT
                     NAPNTEIRSCRLEISNLLNQAETLMNERDSLVSRLRAFITPDEGDKNIQRYRLMQFWR
                     RQIEQKAITKEIPVSPHVDFEYGTEAAIHSLHNVIVQLLRNTFAHGVESPEIRQRAGK
                     KAELTISLQSRLKHDFIEFIYTEDGRGFNQITDENEVPLESIFQKQLTPSMQTANLEA
                     GRGLGMEYIITTIHELEAHAYVQSNAERTVLRIILLR"
     gene            complement(3715..4491)
                     /locus_tag="PLY93_05460"
     CDS             complement(3715..4491)
                     /locus_tag="PLY93_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRP68961.1"
                     /translation="MQTAQDFFLSLLPEATEISKDVSQRYFKKPLDQIGIQHKADASP
                     VTAADIEIESRLRSLIEKKFPDHGIVGEESGNTKSDADFVWVLDPIDGTKSFISGVPL
                     FTTLIALLHKGNPECGAIYQPILNELVWGNNRECFFNGNKTQIRNTAVLKDATLLITD
                     ANDMTTHHPTAQFSKLTAQTRFWRTWADGYGYALLATGYADVMLDPVMNPWDIMAVIP
                     VVRGAGGIVSDFAGNDAAKGTSLMAAPPALHHQVKNILFS"
     gene            complement(4507..5349)
                     /locus_tag="PLY93_05465"
     CDS             complement(4507..5349)
                     /locus_tag="PLY93_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRP68962.1"
                     /translation="MLSKKRLTQAAFVVFLFWALGCASLVKKIFDPPQVGLDRVEIQN
                     VDFTGADLLLYIKINNPNSIGATIKSIDYAFDIDGERLVKGTKNEKTEIAANDLSIIQ
                     VPVSLRYAGLKSGVLGALSKKSLPYVLSGTVVLDTPVGALSFDIGNKGDIPVPDRPRF
                     EIEKIALSEFSVTSTTLMVHIRVTNNHDIALDIEKFRYEFSLQDKLISATDVQVSRSI
                     DTNKSMAVVLPITVKLLGLKQSIVDMIRSGQIKYAMKFDLNLNTRFGPFTIPYERDGL
                     TALY"
     gene            complement(5416..5751)
                     /locus_tag="PLY93_05470"
     CDS             complement(5416..5751)
                     /locus_tag="PLY93_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRP68963.1"
                     /translation="MSATTATEEVIEEIREKVIEQLRTCYDPEIPVNVYDLGLIYGID
                     VDDQKNVHVDMTLTSPNCPSIEELPEEVKEKIESIDGVGTVNLELVWDPPFHMGMMSD
                     EAKLQLGLM"
BASE COUNT         1518 a         1230 c         1436 g         1686 t
ORIGIN      
        1 aagcggtcga atgtgtaatg ggggtttaaa tcgattctgt tggggaatgc agcctctgag
       61 ggtttcgctt tgggagttgt tagcgtggcg gcaggctgtg gagttttttc ttgctttgtg
      121 tgtgttgtaa ttatttttcc ttgaaagccc agcgaaattg cgtcgtcttg tatcgctctt
      181 gcgtattgcg cttgaacgtg tttggcaatt tcgtggctgg gggcagtgag tattaagtca
      241 ttgcccttta tttctgcttt gatgtcttga aaaaaaattt gaaaaacctg cgaaggaagt
      301 ttcttcttta atttgtcgcg tagcgtgagc caaaggcgac tatctgaaga taaaagaagg
      361 ggatcggtag agtcggcaat gtgggaatca gcattgagca taggggagtt ttagaaaatg
      421 gataatagtt tcaagcgagt tgagttaaat taaacacaat ttctctcgaa aatcagactc
      481 gcttactgtt acattttgtt acagagatta tgtcacatta tcgtaccata ctaaaaaaac
      541 tgacgcgata agatttatgt tctgtagatt tattcttcaa gaatgagaag cgcttcaaat
      601 tgttcaaaaa taaaacggtg agcgcttctg cggctgttct cgttaaaccg taacggatag
      661 ttttctcctt taagagcggt tggtagagaa aagtcgtaac gcaccgccga ttgacgctgc
      721 gctgtgccga caataatgtt caaaagttcg ccgacagtat ccatgagcat gcggtcttca
      781 tcggactggg ggattgctaa ttttttcatg gcgatcttaa tgccaagttc tgtcatgttg
      841 agccacagcg ttgcgggaga agaaccggtg agttccattt tggcggtgac gctttggaat
      901 ttttcgggta gcgtaatcat ctcttcgctg atgagctctt gcacaccgat aaaatcacga
      961 aagacatgac ggagaatagt aataaaaata ttaaaatcca gcatttttga aaaacatctc
     1021 aatttgattc cacagctttg gaccgcgcac tggcttttcg atgtagctgt tgacgccgac
     1081 tgcggcggct tgtaaaatct tttcactgtc tgtgacagag gtaatcataa tgatgggtac
     1141 cttattgcca ctgccacgga cttcacgcac aaagtcaatc ccatctaaaa cgggcatgtt
     1201 ccaatcgaga aaaattaatg caactgtgtt tgtctgcata atttccatcg cgtcggcgcc
     1261 attggcagca tgaaaaaatt gtactttatc gagcgcatgg cctaaatttt cacgcacggt
     1321 ttgttcaaca atggtgcgca tgagttcaga atcgtcgaca atgagaaggt tcatcggcta
     1381 ccgtaataga ataatgcgca aaactgtacg ttcggcgttt gactgcacat aggcgtgcgc
     1441 ttctaattca tggatagttg tgataatata ttccatacca aggccgcgtc ctgcttcaag
     1501 atttgccgtt tgcatcgatg gtgtcaactg cttttgaaaa atactttcga gagggacttc
     1561 gttttcatcg gtaatttggt tgaacccccg cccgtcttct gtatagataa actcgataaa
     1621 atcgtgcttt aggcgacttt gtagagaaat tgtgagttcg gcctttttac ccgctctctg
     1681 acgtatctct ggactttcaa ctccatgcgc gaatgtgttg cgcaataact gcacaataac
     1741 gttgtgtagc gagtggatag cagcttcggt accgtattca aaatctacat ggggactaac
     1801 gggtatttct tttgtgattg ctttttgttc gatttgcctg cgccagaatt gcatgaggcg
     1861 gtatcgttgt atgtttttgt cgccttcatc gggggtgatg aacgcacgca gacgcgaaac
     1921 cagactgtcg cgctcgttca ttagcgtctc agcttgattg agtagatttg aaatttcaag
     1981 ccggcaacta cgaatttcgg tattgggcgc gtttgttttg gccaacactt gctcttctaa
     2041 gaggtgcaga gtttcggcca aacttgcaaa atcgagttgc agtgcattgc ctttgagtgc
     2101 gtgcacgcgt tgtgcgaaat cgacgaacgt tgcattcgag acattgtcaa aacgggcaat
     2161 aatttctttg ccgcgacgaa tgatttcttt ttgctgaaca agaaagtttt caagaatctg
     2221 aggaggaatt tgcatccatt gaataagtat atcaaactca aaacgcttct ggttttcaga
     2281 ggcttcaatt tttgctttga gagtaaattc gtctgtttcg tcgacgatgc taaccaaaat
     2341 ccgttttaac ggcgttctcg tcgacgtttc taaagggcga aaagcaaaac ggagaaattt
     2401 cttttcgtgc aagaaatttg tgtaatgaat ttctgtgaga gggttggctt gttcgagcat
     2461 ctgtggactc acgacatttt cgccaaaaaa taaagagaga tattgttcca acgatgcttg
     2521 taacttttta tcttcaaaaa gagacgagag ttttttgtgg gcaatttctt ttagaccaaa
     2581 aattgccaac gcgcgctgcg aatgctcggg ttgtataaaa cctcggctat cgacagtcaa
     2641 aattccctgt tccaaactat ctaagatgcc tgatatttct tgggtgcgtg tgaagatacg
     2701 ctcttctaaa ttttgcgaat actcacccaa ctcgtgcgcc atgagattca ttgcgtacgc
     2761 taaatcgcca aactcgtcgt tggtgtcttc ttcaaggcgg attgaaaagt cgcgttgacc
     2821 aaaatgtgca gcggcctttt gcattgcatc gagaggcttt aataaaaatc gactggtgat
     2881 cgacgcggca agaatggaga ataaaaaaac agcaacgacg atataataac cggtaatctt
     2941 taatccaaat aatgcatcga attcgctggc ggcggaaaaa tcaatcacca aaagcgcgat
     3001 aattttgccg cgcgaattta ttattggagt gtatcctgaa atatagacgc ccaaactttc
     3061 tgtacggtag ccgttggaaa cggtggtctc tcctttgagt gcggatgcaa tactgttttg
     3121 accatgtccg ttgaaaacat cgccgatgtg gtaaatactt tcttcgggat cgatacgatg
     3181 gttgttgttt ctgtcgattt cttgtatctc gtcggcgacc aacacacgta ataagtcggg
     3241 tgcgtatgca tgcatgtgaa ctccggcaat ccatatccgg tgaatttgta cattctggtc
     3301 gagaagcgcc gaggctgcga tggtcgaatc gtacgttggt tttttacccg acgcatagcg
     3361 gatgcgcctt aacttttgca taataatttg aaaactggtc gaacgactaa tcgccatctt
     3421 ttcgttttgt gaaagcgcac ccaagcgcgc tcccgattgc ggttcgggag gcagggcgcg
     3481 cggtacggcg ttgatatcga tgtctaagtt ttctaaaaac aggatatctg cgctcgaaag
     3541 agtggtgacg ccgatcttac caaaatcttt cacgcggctt ttcatctgcg cccacacttg
     3601 ttctgaagcg actttgtagt aatacgtaag gccggtaata gtggcgctaa aagcgacaaa
     3661 gaaaagagcg gtgtttaggc ggacacgaaa attaaaaaac cgccgtttca tacgctatga
     3721 aaaaagaata tttttgactt gatggtggag cgcagggggc gctgccatga gcgatgtgcc
     3781 tttggcagcg tcgttgcctg caaaatcaga gacgatgccg cctgcgccgc gcaccaccgg
     3841 tataactgcc ataatatccc atgggttcat aaccggatcg agcatgacgt ctgcgtagcc
     3901 tgtcgctaaa agcgcatagc catagccgtc cgcccaagtc cgccaaaaac gcgtttgggc
     3961 tgtcagtttt gaaaactgcg cggtagggtg gtgtgtcgtc atgtcgtttg cgtcggtgat
     4021 gagtaacgtg gcgtctttca agacagctgt attccttatt tgggttttat tgccgttaaa
     4081 aaaacattcg cggttgttgc cccaaaccag ttcgttgaga atcggttgat aaatcgctcc
     4141 gcattctgga ttccctttgt ggagaagcgc gataagagtt gtgaaaagcg gcacgcccga
     4201 aatgaaactt tttgtgccgt cgatgggatc caacacccaa acaaaatcgg cgtcgctctt
     4261 cgtattgccg ctttcctcgc ccacgatgcc atggtcgggg aattttttct caatgagcga
     4321 gcgcaaccgc gattcaattt caatatccgc cgctgtaacc ggcgatgcgt cggctttgtg
     4381 ctggatacca atttggtcga gcggtttttt gaaatagcgt tgggaaacgt ccttactaat
     4441 ttcggtcgct tctggtaaga gagataagaa aaaatcttgg gctgtttgca tagggtttcc
     4501 ttaaagttag tagagcgccg ttaaaccgtc gcgctcgtaa gggatggtaa atgggccaaa
     4561 gcgcgtgttg aggtttaagt cgaacttcat tgcgtacttg atttgtcccg agcgtatcat
     4621 gtcaacgata ctctgtttga gccccaagag tttaacggta ataggtagca ccaccgccat
     4681 cgatttattg gtatcaatcg aacgagaaac ttgcacgtcg gtggctgaga taagtttgtc
     4741 ttgtaacgaa aattcatagc ggaatttctc aatatcgagc gcgatatcgt ggttgttcgt
     4801 cactcgaatg tgcaccatga gcgttgtcga agtaaccgaa aattcactta aagcaatttt
     4861 ttcaatctcg aagcgggggc gatccggcac aggaatatcg cccttattcc caatgtcaaa
     4921 cgatagcgca ccgacggggg tatcgagcac gacagtgccg cttaaaacgt agggtaacga
     4981 ttttttagag agcgcaccca gaacgccgct ctttagtcct gcgtatcgta gcgaaacggg
     5041 aacttggatg atgcttaaat cgttggcggc gatttctgtt ttttcatttt tcgtgccttt
     5101 aacgagacgc tccccgtcga tgtcgaatgc ataatcgata cttttaatcg tcgcaccgat
     5161 cgagttgggg ttattgattt taatataaag cagtaaatca gcgccggtaa aatccacatt
     5221 ttgaatttca acgcggtcta aaccgacttg gggaggatca aagattttct tgaccagtga
     5281 agcgcagccg agggcccaaa aaagaaaaac gacaaacgcc gcttgagtta atcgtttttt
     5341 agaaagcata tttaaggcag aagctgcact caacttccga ttgtaaagca gcaatcgaaa
     5401 gacggttaga agaaactaca tcagcccaag ctgaagtttg gcttcgtcgg acatcatgcc
     5461 catgtgaaat gggggatccc acaccaattc aagattcacg gtacccacac cgtcgataga
     5521 ttcgattttc tcttttactt cctctggtaa ttcttcaatc gaagggcagt tgggcgaggt
     5581 gagagtcatg tcgacgtgca cgtttttttg gtcgtccaca tcgatgccgt aaatgaggcc
     5641 taaatcgtac acattcacgg gaatctcggg gtcatagcaa gtgcgtagtt gctcgatgac
     5701 tttctcgcgt atctcttcaa tcacttcttc tgtggcggtg gtcgcgctca tatattatct
     5761 ccgtttttat attcaaaaaa attagcggcg gtttggtcgg gcaaagtctc gtaaaattgt
     5821 ttttgcgacg aaagaattaa aatagaatcg gcatcggtgg gggaaatgcc
//
LOCUS       DAOMPD010000120         5864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5758_length_5864_cov_2.578069, whole genome shotgun sequence.
ACCESSION   DAOMPD010000120 DAOMPD010000000
VERSION     DAOMPD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_5864_cov_2.578069"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLY93_05475"
     CDS             complement(<1..512)
                     /locus_tag="PLY93_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRP68964.1"
                     /translation="MAKIAVMTQLSPTMKEGVFVEWLKNEGDSVRPGDTLASIETDKA
                     VMELEAFDSGVLLKKIAQKGDKIPVGAPIGILGNAGENIDTLLADLKTSTPKPAALPP
                     LPPSTKADLSSGTEKLSTLNHNNTGSSQSNLVNETPPFPPHGRIVASPLAKKRAAQEG
                     IDLTRIQGTGP"
     gene            625..3357
                     /locus_tag="PLY93_05480"
     CDS             625..3357
                     /locus_tag="PLY93_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRP68965.1"
                     /translation="MYKLFKLIKTYWIPTTAMLMVFVFALATHVNLYAQPERFIGKKI
                     KKISFKGNIHVKNETIYNDIIQMKEGQNLTLPLLNGDIKALFNEGSFAYARVEGEDAD
                     GGGVNLTFILEERPRVREVTFLGLEELSATDVQQAIPLKEDDIFTERKLSDSIQKILG
                     KYREKGLFNANVRARKSVVDKTRNTIDITFVVDEGEQIKIAKINLLGINKVDPEDVRA
                     VLELEEDGFIADGTFKSDVFEKDKNTILDFYKTQGFLDVELEDARYDYRWKNPKTQEE
                     RVIVITYKIKEGEQYFFNGYDLVWDERFLNPTTKKQLFTKDHIEEYFEYTKNDVGSVF
                     DYGKFMRDRGIVNYLYSQEGYIYARVQPERTVITLTEEAIADKRNSSQQKEAEKDNID
                     YYNLARLTQILKDNPEMRGRKFVHTRFTIAEGDKGYIENIIVKGNKKTLDKVIRRELL
                     VKEGELFNSELVQRSRERVMNLQFFKEVNVDARPGSSEGKMNLVIDVEEQPTGTISLG
                     GGYGTTTGFSIFTEVAENNLNGTGQRISGRVEFGPLRTMLEASWMEPWLFDKPWSLSL
                     SAFYISRRVLAGSINIATNDESATYNLDTMGFTVGIGHRFLVNWGHYHRYSPQISIAA
                     NASSMVDDSVFLLVQRGWQFKNMITNGIYYDNRDNIFNTTRGMRFEFSHDFVGNILGG
                     QDHFMRYYPQFDFFWWMMDFTFFNTIRKNVLRRWRIVNEVRASATFTQPTSPVWKSQD
                     NTVDPYVEYQDRLFLGGYESLRGWTLFDTLYGIDKNGQVNAAGGTWRNGGSHRILFGS
                     EIRVPVEPSLFWLVLFFDAGALYQNPSEYAFAASAQPALMQAYNDSSLNARNFSLNYF
                     RYSWGFGFRLQIPILPLRIYLAKRLLWDDQAGWFRNHPSQDGFEFVFGIGDRRF"
     gene            3360..>3539
                     /locus_tag="PLY93_05485"
     CDS             3360..>3539
                     /locus_tag="PLY93_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68966.1"
                     /translation="MAARLVLILTASLDEALKIFKTHSNFVSFFENLEIQTQTFQIEN
                     ESFFRYSAPVERTDEL"
     assembly_gap    3540..3549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3550..4257
                     /gene="serB"
                     /locus_tag="PLY93_05490"
     CDS             <3550..4257
                     /gene="serB"
                     /locus_tag="PLY93_05490"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRP68967.1"
                     /translation="SIILTEKQKIIAYKITTTPTSPIRLAFFDTDSTFINEEVIDELA
                     AYAGVKDEVAQITERAMRGELDFDSALKERVSLLKGIPENVFSEIRKNRISLTRGARE
                     LTQYFKEKDIAAYLVSGGFSYFTQSIADELNLTGQFANTLESIDGILSGNTLGRIVNR
                     ERKGEILQELAKKHRVSLENVLAVGDGANDLQMLHLSALGIAFCAKPALLNETTAAIF
                     ERDLRFVRYLIDTPNGV"
     gene            4254..>5864
                     /locus_tag="PLY93_05495"
     CDS             4254..>5864
                     /locus_tag="PLY93_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRP68968.1"
                     /translation="MTDLKTLFEKNYTQFQNRHKILGVFSPNALWDLLTSGAIKALTE
                     GIDKGEWKYKNKYLHSPRNAVREAERNSTEYENAQALIALSLGAGYLLLALKPTVKNI
                     LLIENNPYVFVAFILSGLFEKYTGEITLFYNNLERDDALEAILPWLQGKNLKRTFIYY
                     HAPLWELDKQRMTRAYERVKQLFEKRSVNQATIIKFQQLWNKNIFLNRKGIINSRTLH
                     SLWENKPPHTIIIAGAGPSLSESIAALQKFRDRYILFAADTAFIPLAKADIFADAVFS
                     ADPQWINHYFSQSSLAQRALWAMDPVVCPAIVRYVQSVGAQTLFWNNVFLADTHYRTI
                     DRGDVAHGGSVSTNAFDAARAWLYRRSENENRARLILIGQDLSFSNRQAHVKGAVLEA
                     HIFARNNRLHGFERHNLRQMTAMPTLWRKGIRSAKVRTNGKLAMFIDWFEMQAADTDT
                     KRVQLINATHDGAYLKGFEHTTLADALKDADRVSPIQISKQKIVSLKPLGILDAHLKK
                     IYRLMTENTSLALKVNPTRAEIDCLNKND"
BASE COUNT         1636 a         1351 c         1322 g         1545 t
ORIGIN      
        1 gggcctgtac cttgaatgcg cgtcaaatca atcccttctt gcgctgcccg ctttttcgcc
       61 agcggcgaag cgacgatacg tccatggggg gggaagggtg gcgtttcatt aactaagttg
      121 gattgacttg agccggtgtt attgtgattc aaggtagata acttttccgt acctgaagat
      181 aaatccgctt tagttgaggg gggtaagggg ggcagggcgg cgggtttcgg tgtactcgtt
      241 ttcagatctg cgagtaaagt gtcgatgttt tcacctgcat tccccaatat accgatcggc
      301 gcccccacgg ggattttatc ccctttctgt gcaatttttt tgagcaacac gccgctatcg
      361 aaagcctcga gttccatcac cgctttgtcg gtctcaatcg aagcgagtgt atcgcccggt
      421 cttacgctat cgccttcgtt cttgagccac tcgacaaaaa ccccctcttt catcgtgggg
      481 gaaagctgag tcattaccgc aatttttgcc atagttcaaa caagtgagta aagtacgacc
      541 gtcaagtctt atcgaagcat tgacaacctc tctaaaaaaa cagtttcgac ttaacgtaat
      601 tatattttat tggaaaaagt tctcttgtat aagctcttca agttgattaa aacgtattgg
      661 atacccacca cagcaatgct gatggttttt gtttttgctt tggctacgca tgtcaatcta
      721 tatgcacaac cagaacgctt tattggtaag aaaatcaaaa aaattagctt taagggcaat
      781 atccacgtta aaaacgaaac tatctacaac gatatcatcc aaatgaaaga agggcaaaat
      841 ttaaccctcc cccttctcaa tggagatatc aaagcgcttt ttaacgaagg ctcgtttgcg
      901 tatgcacgtg tcgaagggga agacgccgat ggtggtggag tgaacctcac tttcatttta
      961 gaagaacgcc cccgcgtgcg cgaggtaact tttttaggcc tcgaagaatt atctgcaacc
     1021 gatgtgcaac aggcaatccc tcttaaagaa gatgacattt ttaccgaacg taagctctcc
     1081 gattcaatcc aaaaaattct ggggaaatac cgtgaaaaag gtcttttcaa cgccaacgtc
     1141 cgtgcccgca aaagcgttgt cgataaaacg cgtaacacca tcgatatcac gttcgttgtc
     1201 gacgaaggcg agcagataaa aattgccaaa ataaatcttt tgggtatcaa taaagtcgat
     1261 cccgaagatg tgcgcgcggt tttagaatta gaagaagacg gttttatcgc cgacggtaca
     1321 tttaagagcg acgtttttga aaaagataaa aacaccattc tcgattttta caaaacccag
     1381 ggcttcttag acgtcgaact ggaagatgcc cgctacgatt accgctggaa aaatcccaaa
     1441 acgcaagaag agcgtgtcat cgtcatcacg tataaaatca aagaaggcga acaatacttt
     1501 tttaacgggt acgatttagt gtgggatgaa cgcttcttaa acccgacaac aaagaagcaa
     1561 ttatttacaa aagatcatat cgaagagtat ttcgagtaca caaaaaacga cgtggggtcc
     1621 gtattcgatt acggcaagtt tatgcgcgac cgtggcatcg tgaactatct ctactcgcaa
     1681 gaagggtata tttacgcacg cgttcagccc gagcgtacag ttatcaccct caccgaggaa
     1741 gcgattgccg ataagcgcaa ctcgtcgcaa caaaaagaag cggaaaaaga taatatcgac
     1801 tattataact tagcccgctt gacccaaatt ctcaaagaca atcccgaaat gcgcggacgc
     1861 aaatttgtac acactcgttt tactatcgcc gaaggcgaca aagggtatat tgaaaatatt
     1921 atcgttaaag gaaataaaaa aactttagat aaagtcatcc gccgagaact tttagtcaaa
     1981 gaaggcgaac tctttaattc agaacttgtt cagcgttcac gcgagcgcgt catgaattta
     2041 cagtttttca aagaagtaaa cgtcgatgcg cgcccgggtt catccgaagg taaaatgaac
     2101 ttagtcatcg atgtcgagga acaaccaacg ggcactattt ctttaggcgg tggttatgga
     2161 acgactaccg gtttttctat ttttaccgaa gttgctgaaa ataatttgaa cggaaccggt
     2221 caacggattt caggtcgcgt tgagtttggt cctttacgca ccatgctcga agcatcgtgg
     2281 atggaacctt ggctctttga taaaccatgg tcgctttctc tctcggcgtt ttacatcagt
     2341 cgtcgtgttc ttgccggttc aattaacatc gccaccaacg acgaatcggc cacgtataac
     2401 ctcgacacga tgggcttcac cgtcggtatc ggccaccgct ttttagtcaa ctgggggcat
     2461 taccaccgct attcgcctca aatttccatt gcggcaaacg cttcgagtat ggttgacgat
     2521 tcggtttttc tcttagtaca acgcgggtgg caattcaaaa atatgattac aaacggtatc
     2581 tactacgata accgcgacaa tattttcaac acaacgcgag gtatgcgctt tgagttctct
     2641 catgattttg taggtaacat tttgggtggc caagatcact tcatgcgcta ctaccctcag
     2701 tttgattttt tctggtggat gatggacttt acttttttca atacgattcg taaaaacgta
     2761 ttgcgtaggt ggcgtatcgt caatgaagtg cgggcatcgg cgacgtttac acaaccgaca
     2821 tctcctgtgt ggaaaagcca agacaacact gtcgatcctt atgtcgaata ccaagatcga
     2881 cttttcttag gcggctatga gagtttacgc ggatggacgc tttttgatac gctttacggt
     2941 atcgataaaa atggccaagt aaacgccgcc ggtgggactt ggcgtaacgg ggggagccac
     3001 cgtattcttt ttggttcgga aattcgagtg ccggttgaac cttcgctttt ttggttagtg
     3061 ctctttttcg acgctggcgc gctctaccaa aacccttccg agtatgcgtt cgctgcctcg
     3121 gcacaacccg cgctcatgca agcgtacaac gattcaagct tgaacgcgcg taatttttca
     3181 ctcaactatt tccggtacag ttggggtttt ggtttccgtt tacaaatacc gattctacca
     3241 ctgcgtattt atttagccaa gcggctttta tgggacgacc aagccggttg gtttagaaac
     3301 caccccagcc aagacggctt tgaatttgtc tttggtatcg gcgaccgtcg tttttaatca
     3361 tggctgcgcg ccttgtttta attttaacag cctcgctgga tgaagcgtta aagattttta
     3421 agacacactc gaattttgtt tcattttttg aaaatttaga gatccaaact caaacttttc
     3481 aaatagagaa tgaatccttc tttaggtatt ccgcccccgt cgaacgtacc gacgaacttn
     3541 nnnnnnnnna gcatcatttt aacggaaaag caaaaaatca ttgcgtataa aattacaacc
     3601 acaccaacat cgccgatccg cttagcattt tttgacaccg actcgacgtt catcaacgaa
     3661 gaggttatcg acgagttggc ggcgtacgcg ggcgttaaag acgaagtcgc gcaaattacc
     3721 gaacgcgcca tgcgcggcga attagatttt gatagcgcat tgaaagagcg cgtgtcgtta
     3781 ctcaaaggca ttcccgaaaa tgtgttttcc gaaatccgta aaaacagaat ttcgctcacg
     3841 cgcggtgcgc gcgaactgac acaatacttc aaagaaaaag atattgcggc gtacctcgtc
     3901 tccggcggat tttcttactt cacgcaaagt atcgccgacg agttaaatct cacaggtcaa
     3961 tttgctaaca cgttggaaag catcgacggt attctcagcg gcaatacact ggggcgcatt
     4021 gtaaaccgtg agcgcaaagg cgaaatttta caagagctgg caaaaaaaca ccgtgtatcg
     4081 ttggagaatg tgcttgccgt cggcgacgga gcaaacgatt tacagatgct ccatttgagt
     4141 gcattgggga ttgccttttg cgccaagccg gctctcctca acgaaaccac cgccgctatt
     4201 tttgaacggg atttacgctt tgttcggtat ttaatcgata cccctaacgg cgtatgacgg
     4261 acttaaaaac actttttgag aagaactata cccaattcca aaaccgccat aaaatccttg
     4321 gagtattttc acccaacgca ctctgggatt tattgacgag tggcgcgata aaagcactca
     4381 ccgaggggat cgataaagga gagtggaaat acaaaaataa atatttgcac tcaccgcgca
     4441 acgccgtccg cgaagccgag cggaactcta ccgaatacga aaacgcgcaa gcgctcatcg
     4501 ctctttcact gggggctggg tatcttttgt tggcattaaa gccgacggta aaaaatatat
     4561 tgctcatcga aaataatcca tatgtgttcg tggcgtttat tttaagcggt ctttttgaga
     4621 aatatacggg agagataact cttttttaca acaatttgga gcgcgacgat gcactcgaag
     4681 caattttgcc gtggctacaa gggaaaaatc tcaagcgcac gtttatctat taccatgcgc
     4741 cgctttggga actggataaa cagcgcatga cgcgtgcgta cgagcgggtg aagcagcttt
     4801 ttgaaaaacg ctcggtgaac caagcgacga ttattaaatt ccaacagctt tggaataaaa
     4861 atattttctt gaaccgtaaa gggattatca actctagaac gttacactcg ttgtgggaaa
     4921 ataaaccgcc tcatacaatc atcattgcag gggcagggcc gtcgctttct gagtctatag
     4981 ccgcactcca aaaattccgc gaccgctaca ttttattcgc cgccgatacc gcatttattc
     5041 cgttagcaaa agcagatatt tttgccgatg cggttttttc tgctgatcca cagtggatca
     5101 accactattt ttcacaatca tcgttggcgc aacgcgcttt atgggcgatg gatcctgtgg
     5161 tgtgtccagc gattgtgcgg tatgtgcagt cggttggcgc acaaacactt ttttggaata
     5221 atgttttttt agccgacaca cactaccgta caatcgatcg tggggatgtc gcccacggtg
     5281 gttcggtctc gacgaatgca ttcgatgccg cacgcgcgtg gctttacaga cgtagtgaaa
     5341 atgaaaacag agcacgcctc atcctcattg ggcaagacct ttcgttcagt aaccgacaag
     5401 cgcatgtgaa aggggcggta cttgaagcgc acatctttgc gagaaacaac cggttgcatg
     5461 gttttgaacg ccataactta cgtcaaatga ccgcgatgcc aacgctctgg cgaaaaggca
     5521 tccgtagcgc aaaagtacgc acgaatggca agctggcgat gttcatcgat tggtttgaaa
     5581 tgcaggctgc cgacaccgat acaaaaaggg tacagttaat taacgccaca cacgacggtg
     5641 catacctcaa ggggtttgaa cacaccacgc ttgccgatgc actcaaagat gctgatagag
     5701 tctctcccat tcaaatttca aaacaaaaaa tagtgtcgtt aaaacctttg gggattttag
     5761 acgctcacct caaaaaaatt tatcgactca tgaccgaaaa tacgtcgctt gccttaaaag
     5821 ttaatcccac ccgcgctgaa attgattgct tgaataaaaa cgac
//
LOCUS       DAOMPD010000121         5835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5800_length_5835_cov_2.601211, whole genome shotgun sequence.
ACCESSION   DAOMPD010000121 DAOMPD010000000
VERSION     DAOMPD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5800_length_5835_cov_2.601211"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..969)
                     /locus_tag="PLY93_05500"
     CDS             complement(109..969)
                     /locus_tag="PLY93_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP68969.1"
                     /translation="MPTLYEKGIRDEKLADAHDAQAFFGSMQILRWQVDNPLKNYQYI
                     IVDTHRKAILIDPLDVMAIQKIIESEALFPAAILITHEHHDHAGKVKQIQGNWSIPAY
                     ASEESAALFEGDTTIVHDKENIPISLDFKIIPHFTPGHTAGHTAYEIGGFLFSGDALM
                     HGGCGHCRSANSNIEEHYATINERLNTLSPDLIVMPGHYYAARNLDFTLHIEPENKRA
                     HEMRARLQTESDELEHQTTLKQEKEYNVFMRLGQRNVRAHTAALIEKNLSEVNDLTVF
                     KELRTLRDQW"
     gene            complement(927..1709)
                     /locus_tag="PLY93_05505"
     CDS             complement(927..1709)
                     /locus_tag="PLY93_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP68970.1"
                     /translation="MIYSKNDFVGFYTIVRKEYIRIVRIWTQTLLPPVMNVALYLLIF
                     GAFMGNRIGQVGNDSYASFIIPGLILMAVITNAYGNVSTSFFSMKFQKSIEEILVSPI
                     PESLVVIGFTFGGIIRGLFVAFLVFLVTLVAPGIQLKFINPFFAILVLVLTATFFSLA
                     GLLNAMLAKKFDDVSFIPSFVLTPLTYLGGIFYPLKSLPEVWQNIAMLNPIQHMVSSL
                     RFGFLGTSELPLWPSILMLVCGNIILYLVCLRYMKKGLGMRN"
     gene            complement(1706..2611)
                     /locus_tag="PLY93_05510"
     CDS             complement(1706..2611)
                     /locus_tag="PLY93_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP68971.1"
                     /translation="MITALRKTYANHFEALKGVDLTVQEGDFFALLGPNGAGKWTLVS
                     ILASLSLKTSGDVKIFDADIDRDIERAKSYMGLVPQEFNLNHFETPLQILVNQAGYYG
                     IPPRLAKARAEKYLNDLQILDKATIQARMLSGGMKRRLMIARALIHEPKILILDEPTA
                     GVDIELRKLIWDFLTALNKNGTTIILTTHYLEEAENLCRNIAIIDQGEIIANTSMKKL
                     LGDLAEQLYIVEFTKPIKKIPILKDFTVERIDNHTLQIVVERKQNLNTLFARLTEKKL
                     FVESIREKTNRLEQLFLDLTGKYSP"
     gene            complement(2627..3571)
                     /gene="gshB"
                     /locus_tag="PLY93_05515"
                     /pseudo
     CDS             complement(2627..3571)
                     /gene="gshB"
                     /locus_tag="PLY93_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014947.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
     assembly_gap    3651..3697
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3698..4340)
                     /locus_tag="PLY93_05520"
     CDS             complement(<3698..4340)
                     /locus_tag="PLY93_05520"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRP68972.1"
                     /translation="MNHDSSAGTAQWIQEVAIPVLPTWRAGWHTRSKARHFTHYAALA
                     TELGGIIGVDPWFLSPLGVRLNDVDINEDASRSRIAAHAETLLKAIQAKYNEYQIGEK
                     PFIFLKSDHGTYGMAMLSFDNAAEILEINRKEKNKLFKGKSSVVVKDYLLQEGIPTIL
                     KDAVPGGKIAYTAESVIMLANNRFIGKFLRVNEQKDTRQSLNSTGMFFKPVTLE"
     assembly_gap    4367..4427
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4428..4857)
                     /locus_tag="PLY93_05525"
     CDS             complement(<4428..4857)
                     /locus_tag="PLY93_05525"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF020460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRP68973.1"
                     /translation="MLQNKIITALPRIEEWLTALEKNAPLYMSADIRDAGFKVASIDA
                     NLYPAGFNNLDPTSHGLATEYLKAALSHNSIQPPARLLLVAEEHTRNLYYLENIHVLR
                     NLLENAGFTVTITTAFELEGALYSNGETHLQSASGSIVSLL"
     gene            complement(4871..5182)
                     /gene="grxD"
                     /locus_tag="PLY93_05530"
     CDS             complement(4871..5182)
                     /gene="grxD"
                     /locus_tag="PLY93_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRP68974.1"
                     /translation="MNIEEMKTKIAEDVKANPVVLFMKGSKEFPQCGFSARVVQILNA
                     HNADYVTRDVLSDPLLRDAIKQFSDWPTLPQLYINGEFIGGCDIVTEMAERDELQPLL
                     K"
     gene            complement(5202..5429)
                     /locus_tag="PLY93_05535"
     CDS             complement(5202..5429)
                     /locus_tag="PLY93_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRP68975.1"
                     /translation="MIHPQEVEALIRAHIEGAQVKVLDPMDDGMHLQAVVISEKFAGL
                     PLMKQHKMVMEAVREPLEGRLHALQITTKTP"
     gene            complement(5440..>5835)
                     /locus_tag="PLY93_05540"
     CDS             complement(5440..>5835)
                     /locus_tag="PLY93_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRP68976.1"
                     /translation="KSLSRKHTPTRETVRQLAPETVRRLAETILLRIIAKLEIVMYIL
                     STMDHNLILYHFDGCGPCRRVKDTIKELNLDIEWRDIRESEDAYNELLKVGKKQQVPC
                     LFIDGNPLYESSAIIRWLRENYTNSPVLS"
BASE COUNT         1430 a         1258 c         1406 g         1633 t
ORIGIN      
        1 gaacgcccag agggcatccg tgcccgcgtt cgagttcgcc ctccgtgcgc ttcactcgaa
       61 cgcccagagg gcatccgtgc ccgcgttcga gttcgccctc cgtgcgcttc accactggtc
      121 gcggagggtg cgtagttctt tgaaaactgt caaatcgttt acttcactta aatttttttc
      181 aatgagtgcg gcagtatgcg cccgaacgtt acgttggcct aaacgcatga aaacattata
      241 ttctttttct tgcttgagcg tcgtttggtg ttctaattcg tcgctctcag tttgaaggcg
      301 tgcgcgcatc tcatgcgccc gtttgttttc aggctcgatg tggagcgtaa aatccagatt
      361 gcgtgcggca taatagtgcc cgggcatcac aattaaatct ggggagagcg tatttaagcg
      421 ctcgttgatg gtcgcgtaat gctcttcgat gtttgagttt gcagaacggc agtggccgca
      481 cccaccgtgc atcagtgcat cgcccgaaaa gaggaaccct ccaatttcgt acgcagtgtg
      541 acccgcagtg tgaccgggcg taaagtgcgg tataatcttg aaatctaaac tgattggaat
      601 gttctctttg tcgtgcacga tggttgtgtc tccttcaaag agcgcagccg attcttccga
      661 tgcataagcg ggaatactcc aattgccttg gatttgtttg actttccccg cgtggtcgtg
      721 gtgttcgtgt gtaatgagga tcgctgcggg aaataacgct tcactctcga taattttttg
      781 aattgccatg acatcgagtg ggtcgatgag tatcgctttt ctatgggtat cgacaataat
      841 gtattgataa ttttttaacg gattgtctac ttgccagcgt aaaatttgca tagaaccaaa
      901 aaacgcctgt gcgtcgtgcg cgtcagctaa tttctcatcc ctaatccctt tttcatatag
      961 cgtaggcata cgaggtagag aataatattt ccacaaacga gcatgagtat cgatggccaa
     1021 agcggtaatt cagacgtgcc taaaaaacca aagcggagcg aactgaccat gtgttggatg
     1081 gggttcagca tggcgatgtt ctgccatacc tcaggtaaac ttttcaacgg ataaaaaata
     1141 cctcccaagt aagtaagcgg ggtgagcaca aagctgggga taaaactcac atcgtcgaat
     1201 ttttttgcaa gcatcgcatt gagtaaaccc gcaagagaaa aaaaagtcgc tgttaaaaca
     1261 agaacaagta ttgcaaaaaa aggatttatg aattttaatt gaatacccgg ggcaacgagg
     1321 gtaaccaaaa aaacgaggaa cgcaacgaag agcccacgga taatcccacc aaaggtaaag
     1381 ccaataacca ccaacgattc gggtatcggt gaaactaaaa tttcttcaat gcttttttgg
     1441 aatttcatac tgaaaaacga agtcgatacg tttccgtaag cgttggtaat cactgccatt
     1501 aaaatgagcc ctgggattat aaaactggcg tacgaatcgt tgcccacttg gccgatgcgg
     1561 ttgcccatga aagcgccgaa gatgagcaag taaagcgcaa cgttcataac cggcggcaac
     1621 agagtttgcg tccaaatccg cactatacgg atatattctt tgcgcacaat agtgtaaaaa
     1681 ccgacaaaat cgttttttga ataaatcatg gcgaatattt tcccgttaaa tctaaaaaga
     1741 gttgttcgag tcggtttgtc ttttctcgta tactctctac aaaaagtttt ttctcggtga
     1801 gccgcgcaaa gagggtgttg aggttttgct tacgctcgac gacaatttgt aatgtgtggt
     1861 tgtcgatacg ctcgacggtg aaatctttaa gtatggggat tttttttatc ggttttgtga
     1921 attcgacgat gtacaattgc tcggccaaat ccccgagtaa ttttttcatc gaagtgttgg
     1981 cgataatttc accttggtcg atgatcgcaa tattgcgaca aagattctcc gcttcttcta
     2041 aataatgcgt cgtgaggata attgtcgtgc cgtttttatt gagtgcggtt aaaaaatccc
     2101 aaataagttt tcgtagttca atgtcgacgc ctgcagttgg ttcgtctaaa attaaaattt
     2161 tgggttcatg gatgagcgca cgcgcaatca tgagccggcg tttcatacca cccgagagca
     2221 tgcgcgcttg gattgtcgct ttgtctaaaa tttgcaaatc gtttaagtat ttttcggcgc
     2281 gtgcttttgc aaggcgcggt ggaatgccat aataccccgc ttggttgact aaaatttgta
     2341 ggggcgtttc aaagtggttt aagttaaatt cctgaggcac aaggcccatg taagattttg
     2401 cccgttcaat atcgcggtcg atgtcagcgt caaaaatttt gacatcccct gaggttttaa
     2461 gcgacagtga cgccagtata ctgacgagcg tccattttcc tgcaccgttt ggtccaagaa
     2521 gggcaaaaaa atcgccctct tgcacggtta aatcgacgcc ctttagcgcc tcaaaatgat
     2581 tggcgtacgt ttttctaagc gcggtaatca ttaatgcgtt caacggttat tctccttgta
     2641 gggggattaa cttttcggcg gcaacgagaa tttttctcgc taaattttct tcatagtact
     2701 gctgcatttc gcgcagacac gtcggtgaag tcacatttac ttcgataagc ttttcgccga
     2761 tgatatcaat ccctgcaaaa aaaattccat gacggatgag atggggcttt agcgcttcac
     2821 aaatgaggcg ttcgtttgca gtgatggtgg ttttgagcgc ttttccaccg gcggcaaagt
     2881 tgttgcgatg gtcatccccc gaatggtggc ggagcaccgc gcctaaaatt tcgccgttca
     2941 gaaggagtat acgcttatcg ccttcgagcg cagcgggcaa gtacgcttgc acaatcaggt
     3001 aacgactcgt tttttggagt gtttcaaaaa ctaccgcaga gtttttatcg ccgtgcttaa
     3061 cgataaaaac cgaagagccg ccaaaaccgt cggtgggttt cacaaccacg gttttgtgct
     3121 cttccaaaaa ttttttatac gcgtcgatat gggcggttat gagcgtcgct ggcgtgaatg
     3181 ttttaaactg tgtagcccaa attttttcgt tgatggtacg caatccctcg gggttattta
     3241 agaccagcgg ttttttaccc gcttgcgata aaagccaagt gtcggttaag taatcgacgt
     3301 tgaagggcgg gtcggtgcgg ataaaaaaaa tatcaaaaaa attcgccgcg tattcggtat
     3361 aatcggataa cgtaaaagga ttttctaccg agtcggtaac ctctgccgca cgcgcacaaa
     3421 taaaaagcga accttcttag agagtcatat ctccccgttc taaaatatac acactatgcc
     3481 cacgcgcttg cgcctcgatc atgagcgcga acgaggtatc tttttttata tgtaaacttt
     3541 cgggtggatc gattaagaac gcaattttca tgccggtcaa gagtctatag agggtgcggc
     3601 tctttcgccg acgcttcaac gatctcttca atttcacgct gtagcgcaat nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt cgagcgtaac cggcttaaaa
     3721 aacatccctg tgctgttgag gctttgcctt gtatcttttt gttcgttcac gcgtaaaaat
     3781 ttgccgatga agcggttgtt cgccagcata ataacgcttt ctgccgtgta cgcaattttt
     3841 cccccaggga cggcgtcttt aaggatagtg gggatcccct cttgaagtaa ataatctttg
     3901 acgacgaccg agcttttacc tttgaaaagt ttattcttct ctttgcggtt gatttctaaa
     3961 atctctgccg cattatcaaa cgaaagcatc gccataccgt atgtgccgtg gtcggattta
     4021 agaaaaataa acggcttttc accgatttgg tattcgttat attttgcttg gattgctttt
     4081 aatagtgttt ctgcgtgcgc ggcgatgcgg ctgcgcgatg cgtcttcgtt gatgtctaca
     4141 tcgttgaggc gtacaccgag cggcgacaaa aaccaaggat cgacgccgat gatgccgcct
     4201 aactcggtag caagagctgc gtaatgtgta aaatgccgcg ctttactacg cgtatgccag
     4261 cccgcgcgcc aagtgggcaa aacagggatg gcgacttctt gtatccactg tgcagtgccc
     4321 gccgacgaat cgtggttcat aataagtgcg gcaaactcgc tttgctnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta aaaggctgac
     4441 aatactgcca cttgcgcttt gcaaatgcgt ttcaccgttg gagtagagcg cgccttccaa
     4501 ttcaaatgct gtcgttatcg tcacggtaaa tccggcgttt tcgagaaggt tgcgcaagac
     4561 atggatgttt tccaaataat aaaggttccg cgtgtgttcc tcggcgacaa gcaaaaggcg
     4621 tgcagggggt tgaatgctgt tgtgcgagag cgctgctttc aagtactcgg ttgctaagcc
     4681 atggctcgtg gggtcgaggt tattaaaccc tgcggggtat aaattggcat cgatggaagc
     4741 aaccttaaag cctgcgtcgc gtatatctgc cgacatataa agcggcgcgt ttttttctaa
     4801 cgcggtgagc cattcttcga tgcgcggcag tgcggtgatg atcttatttt gcagcaaggc
     4861 gttcaaggtt ttacttcaaa aggggttgca gttcgtcgcg ctcggccatt tcggtgacaa
     4921 tgtcgcaacc accgataaat tcgccgttga tataaagttg gggtagagtg ggccagtcgg
     4981 aaaattgttt gatggcatcg cgcaacaagg ggtctgagag tacatcgcgg gtgacgtaat
     5041 cggcgttatg tgcgttcaaa atttgtacca cacgcgccga gaagccgcac tgagggaatt
     5101 cctttgagcc tttcataaaa aggacaacgg ggtttgcttt tacatcctcg gcaattttgg
     5161 ttttcatttc ttcgatattc atttttcttc ctatttattt tttacggtgt ttttgttgtt
     5221 atttgtagtg cgtgtaatcg accttctaaa ggttcgcgga cggcttccat taccatttta
     5281 tgttgtttca tgaggggcaa tcccgcaaat ttttctgaaa tcacaactgc ttgcaagtgc
     5341 ataccgtcgt ccatggggtc taaaaccttg acttgagctc cctcaatgtg agcacggata
     5401 agggcttcaa cttcttgtgg atggatcata gtggataggt taggaaagga ctggtgagtt
     5461 tgtgtaattc tcacgcaacc aacggatgat ggcgctggac tcataaagcg gattaccgtc
     5521 gataaagagg cagggcactt gctgtttttt acccaccttc aaaagttcgt tgtacgcgtc
     5581 ttcactttcg cggatgtcgc gccactcaat gtcaagattg agttccttga tcgtatcttt
     5641 aacgcgacgg caaggaccgc aaccatcgaa gtgataaaga atcaggttgt gatccatagt
     5701 agataaaata tacattacta tctctaattt ggcaataatt cgcaaaagaa ttgtctcagc
     5761 gagtcggcgc acagtctccg gggcgagttg gcgtacagtc tccctggtgg gtgtgtgttt
     5821 gcgggataaa gattt
//
LOCUS       DAOMPD010000122         5831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5811_length_5831_cov_2.526835, whole genome shotgun sequence.
ACCESSION   DAOMPD010000122 DAOMPD010000000
VERSION     DAOMPD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_5831_cov_2.526835"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="PLY93_05545"
     CDS             complement(<1..1474)
                     /locus_tag="PLY93_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68977.1"
                     /translation="MGLLAKSLKKANAAKTEPFDFESQYERLAELTETAPKPPKVSLR
                     ERAKSFLRRAAHPMGESIDAPHKNSERPKLSLRERARRLLQKIKNEPSGSEGGGGSAS
                     AHRASGGISSHSGTTPVTPMTPPTHSAAALGENGDALPADEETLLYSIFPWNKINAEG
                     ELEPTISFGEYSAEDCGLTGPLSNASPDEENSSRESPSTEPLTTFATTMERITENLRN
                     LHSQLDQGNLIRNVHVESAATPVTNAPQGEFTSHHVSLDTAGEEAHPTRMATENAKDV
                     FWPKTLPIDLPARDIKIGGDGRLSVVPGPFQIPYLAADIIPPESEFSYFHRSLKSAEL
                     FIGEGEIQLAKIIYERLLKKISDKAIKEKIQKNLDALEDYRKSHDWGNFLPVPPWNQN
                     AWQKNPWHEMPEAKVNVSQMPVEAQNITINMDKGFFEIARALFELKDSILEKKGTADG
                     AEKEPSTEGKVDGVDEGGNPAEDLRGGEDRRKGGIDTWGEG"
     gene            complement(1511..2290)
                     /locus_tag="PLY93_05550"
     CDS             complement(1511..2290)
                     /locus_tag="PLY93_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68978.1"
                     /translation="MKRIALVICASIVTLHCGKNYDELFHEGVELRKNHKWSLAKKKL
                     FEAAEKKATAEVYKELGNVFLLGEQNIGEAENYYQKSLVVNPKYINAEFNMAVIQLKK
                     YELTLDNKGQGDAKILDAAHTWFQRVYEQDPNFSIGIEEMAKYYYYKRDYKKALETAQ
                     RAVGLDNRNANAYSVLGQIYFSGLKDYKQAFDNFDQARSLNNNDTDVVYFLWATSVKL
                     NKKEDATQFKMHYEQRLEKEGLTKEQVQDRVKRLENQLRGG"
     gene            complement(2332..3891)
                     /locus_tag="PLY93_05555"
     CDS             complement(2332..3891)
                     /locus_tag="PLY93_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRP68979.1"
                     /translation="MQLSRRDKVVTTVVVAIILVLLYFLQCHCRNQGQGRSDAQPVGV
                     VEYKYHQVQRKFSDSMQWQDVDAKSPVYAYDWVMTKSKSDARITLNNGMKVDMDPDAM
                     IEIDENQAGVGMTLHGGSVRADTRGAKNAKLKTADGTEIELNDARAQIASDGKSLAID
                     VKEGSASVKKEGGDAQRVNAGEIATLEKGNLTKEKVSIRLVKPTDALILDDSSKDVEF
                     VFEKPADAKDCQVLLTGEEGGRTLKATSSPVRARVGEGSYKWRVECKQNEKTITSGTG
                     LFRMRAGAMLEVVYPQPGQTVYANEAQNLALRWKSNTPVQIELADDAAFQNKLRTEKS
                     DNQILAVGTLKPGRYFWRLTPESGAPKVASFSVSEKGELLAETDTQSSKEQPLVKKIE
                     TKTELKNESPKTTKKNQALTTVSKTNYTIEPDARVAQVPISWSPVNAAYTYRVRVAPS
                     KDLVQSVNITPVKGKGKATLSLKPGTHYYRVDAFVQGNKKPVAQSPVKQIQVAQKKLP
                     PPPRVKSVEAD"
     gene            complement(3904..>4413)
                     /locus_tag="PLY93_05560"
     CDS             complement(3904..>4413)
                     /locus_tag="PLY93_05560"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002084985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP68980.1"
                     /translation="NGVVDKFIGDAIMAVWGAPVERENDVENSLLAALEMRAVLHKFN
                     VGRGSKSKPTIRIGMGLNTGELLAGQIGSNDRVEYTVIGDTVNLASRIEGLNKTFGTD
                     ILISENTYKLMRAKFHCIQLDRIKVKGKSEAQRVYAVLGKIKDTSAPKNLAELHRLID
                     LYPQKQKGK"
     assembly_gap    4414..4423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4424..5737)
                     /locus_tag="PLY93_05565"
     CDS             complement(<4424..5737)
                     /locus_tag="PLY93_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRP68981.1"
                     /translation="MLTKLRALYASIFTTIRSKLIAIVSFIIIISVLLFTGISLNMFQ
                     SSMTEMVKLNNGNNAKMLTDKVEGELRTTAEQLRLLTTLSRKSEAVQRSYFSNESDFF
                     LIADFPSLLEEASAVYTRAEKLSRFNLTRSELVAHLKFSEADQKEIREGKTLIRALTS
                     PRQHVWLYVVPATPKGERAIAAIMNGDSLSTLLSAVKSKGAATSLYAAMLVDEKKKLV
                     AHSENRHFKFGQDISKHPIVDVAFKNFAKQVVTGVQRYTIDDTHEYGSYRKLTLGGAL
                     ITTVNEDVALAGVRWARVLTILIALLTIAVAFLFIYFFSRTISDPLKRLVGATKTIRS
                     GNYDVHLEKTSNDETGELTQAFNEMAVGLKERENLKGAFGKFVNKEVAKMVMKGDLSL
                     GGETKTATVFFSDIRSFTSISEKLTPQEVVGFLNEYMTLMVNIVHK"
BASE COUNT         1289 a         1459 c         1338 g         1735 t
ORIGIN      
        1 acccctcccc ccacgtatcg ataccacctt tgcggcgatc ttcgccacct cttaaatctt
       61 cagccggatt tcccccttca tcgactccat ccaccttgcc ttcggttgat ggttcttttt
      121 ctgcgccatc tgccgttcct tttttttcaa gaatcgaatc tttcaattca aaaagagcgc
      181 gggcgatttc aaaaaagccc ttgtccatgt tgatagtgat attttgcgcc tcgaccggca
      241 tttgcgaaac attcactttc gcttcgggca tttcatgcca cggatttttt tgccacgcat
      301 tttggttcca tggcggcacg ggtaaaaaat taccccaatc gtggcttttg cggtagtctt
      361 ctaaagcatc gagatttttc tgaatctttt ctttaatcgc tttatctgaa atttttttga
      421 gcagccgttc gtagataatt ttagcgagtt gaatttcgcc ttcgccgata aaaagctccg
      481 cactttttaa gctacggtga aaatacgaga attcgctttc gggagggatg atgtcggcgg
      541 caagataggg aatctgaaaa ggtccgggga ccacagacaa tcgaccatcg ccgccaattt
      601 ttatatcgcg tgcaggtaag tcgataggga gcgtcttcgg ccaaaatacg tctttagcat
      661 tttcagttgc catccgtgtt ggatgtgctt cttcacccgc tgtatcgagc gaaacgtgat
      721 ggctggtaaa ctcaccttgt ggagcattcg ttacaggcgt ggcggcgctc tccacatgca
      781 cgttacggat tagattccct tggtcgagct gggaatgtaa atttcttaag ttttcagtga
      841 tacgctccat cgttgtggcg aacgtagtta gtggttcagt ggacgggctc tctcgactgg
      901 aattctcttc atctggagag gcgttgctta aaggtccggt aagcccacaa tcttccgccg
      961 aatactctcc gaatgaaatt gtaggttcta actcgccttc ggcgtttatt ttattccacg
     1021 gaaaaataga ataaagaagt gtttcttcat cggcggggag cgcgtcccca ttttcaccca
     1081 gcgcagcggc agaatgtgtg gggggagtca tcggcgttac cggtgtagtt cctgaatgag
     1141 aggaaattcc accgctggcg cgatgagcgc tggcgcttcc gcctccacct tctgatcccg
     1201 atggttcgtt tttaattttt tgtaaaagcc ggcgggctct ttcacgtaag cttagtttag
     1261 ggcgttcact gtttttgtga ggcgcgtcga tagactcgcc catggggtgt gcagcgcgcc
     1321 tgagaaaact cttcgctctt tcacgcaacg agactttggg cggcttgggg gcggtttctg
     1381 ttaactcggc aagacgttcg tattgcgact caaaatcgaa cggctcggtc ttggccgcgt
     1441 ttgctttttt gagcgattta gctaaaaggc ccacacctaa ttatcgacgt ccgagtcgct
     1501 aaacttgtat ttacccgcca cgaagctggt tttctaaacg cttcactcgg tcttgcactt
     1561 gttctttagt taaaccttct ttttctaagc gttgttcgta atgcatttta aattgagtgg
     1621 cgtcttcttt tttatttagt ttcacactcg tcgcccagag aaaatatacc acgtcggtat
     1681 cgttgttgtt gagtgaacgc gcttggtcaa aattatcaaa cgcttgcttg tagtctttaa
     1741 gaccgctaaa ataaatttgc cccaacaccg aatacgcgtt ggcgttacgg ttatctaaac
     1801 ccaccgctct ctgggcggtt tctaatgcct tcttataatc gcgtttgtaa taataatact
     1861 tagccatctc ttcgatgccg atagaaaaat tggggtcttg ttcgtaaacg cgctgaaacc
     1921 atgtatgcgc agcgtcgaga attttagcgt caccctgccc tttattgtct aaagttagtt
     1981 cgtatttttt gagttggata accgccatgt taaactcggc gttgatgtac ttagggttga
     2041 cgacaagcga tttttggtaa taattttcag cttcaccgat attttgctcg ccgagtaaaa
     2101 agacattccc caactctttg taaacctcag cggtcgcttt tttctctgcc gcttcaaaaa
     2161 gttttttctt tgcaagcgac cacttgtggt ttttccttaa ctcgacgcct tcatggaaca
     2221 attcatcgta gttctttcca caatgaagag taacgatgga tgcacaaatg accagggcaa
     2281 ttcttttcat aggcatcgat tacagcgcgg gcaacatagt caacactttc actaatccgc
     2341 ttctaccgat ttcacacgcg gcggtggggg tagttttttc tgcgcgactt gaatttgttt
     2401 taccggggat tgcgccaccg gttttttgtt cccttgaaca aacgcatcga cgcggtagta
     2461 gtgtgtacct ggtttgagcg acagtgttgc tttacccttg cctttcactg gtgttatgtt
     2521 caccgactgc accaaatctt tggatggcgc aacacgcacg cggtatgtgt atgcggcatt
     2581 taccggcgac cagcttatcg gcacctgtgc cactctcgca tcgggttcta tggtataatt
     2641 tgtttttgat accgtcgtta acgcctgatt tttttttgtc gtttttggag actcgttttt
     2701 gagttctgtt tttgtttcta tttttttaac cagcggttgt tcctttgaag attgggtatc
     2761 tgtctcggct aaaagctcgc ctttttctga aactgaaaac gatgcaactt ttggggcacc
     2821 cgattcggga gtaagtcgcc aaaaataccg acctggttta agcgtaccca ccgccaagat
     2881 ttgattatcg cttttttctg tgcgtaattt attttgaaac gcggcatcat ccgccaactc
     2941 tatttgaacc ggtgtgttcg atttccaccg tagggctaag ttttgcgctt cattcgcata
     3001 aaccgtttgc cctggttgtg gatagaccac ctcgagcatt gcacccgccc gcatccgaaa
     3061 caaccccgta cccgaggtga tcgttttttc attttgctta cattcaacgc gccacttata
     3121 gctgccctca cccacgcgcg cgcgcaccgg cgaacttgtc gcctttagtg tgcgcccgcc
     3181 ttcttcgccg gtgagcaaca cctggcagtc tttcgcgtcg gcgggctttt caaacacgaa
     3241 ctcaacgtct tttgagctgt cgtctaaaat gagcgcgtcg gtaggtttaa ctaaacgaat
     3301 tgatactttt tcttttgtga gatttccctt ctccaaagtg gcaatctcac cggcgttgac
     3361 acgctgcgca tccccgcctt cctttttgac cgatgcgctg ccttctttca cgtcgatggc
     3421 gagcgacttg ccgtcagagg cgatttgcgc ccgtgcatcg ttgagctcaa tttccgtacc
     3481 gtctgcagtt ttgagttttg cgtttttcgc cccccgcgta tcggcgcgca ccgagccgcc
     3541 gtgtaacgtc ataccgactc ctgcttggtt ttcgtcgatt tcaatcatcg catcgggatc
     3601 catgtcgact ttcatgccgt tgttgagagt gatgcgcgca tccgacttcg atttcgtcat
     3661 cacccagtcg tatgcgtaga ccggcgactt ggcatcgacg tcttgccatt gcatgctgtc
     3721 cgaaaatttt cgttgtactt ggtggtattt atattcaaca acacctacgg gctgcgcatc
     3781 gctgcgtccc tgcccttggt tacgacaatg gcattgtaaa aaatagagta acaccaaaat
     3841 aatggcgact acaacggtgg tgactacttt atcgcggcgg cttaactgca tatcgattac
     3901 cggttatttc cctttttgtt tttgtggata gagatcgatg agacgatgca actctgccaa
     3961 atttttggga gcactcgtgt ccttaatttt gccgagcact gcgtacacac gctgcgcttc
     4021 ggatttacct ttgactttaa tgcggtcgag ctgaatacaa tgaaatttag cgcgcataag
     4081 cttgtacgtg ttttcgctaa ttaaaatatc ggtaccgaat gttttattga gcccttcgat
     4141 ccgcgaggct aagttcaccg tgtcgccgat cacggtgtat tccacgcggt cgtttgagcc
     4201 gatttgcccc gccaatagct cgccggtatt aagccccatc ccgatgcgaa tggtgggttt
     4261 acttttacta ccgcgtccca cgttaaattt atggagcact gcacgcattt caagcgcagc
     4321 taaaagcgag ttttcaacgt cgttctcacg ctccaccgga gccccccaca ctgccataat
     4381 cgcatcgccg atgaatttat cgaccacgcc gttnnnnnnn nnncttatgc acgatattca
     4441 ccatgagcgt catgtactcg ttcaagaacc ccacaacctc ttgtggagtg agtttttctg
     4501 aaatcgacgt gaaagagcga atgtcggaaa agaaaaccgt agcagtctta gtttcaccgc
     4561 ccaacgataa atcacccttc atcaccattt tcgcgacttc tttgttgaca aatttaccaa
     4621 acgcaccttt caaattttcg cgttctttaa gtcccactgc catttcatta aacgcttgag
     4681 tcaactctcc cgtttcatcg ttcgatgtct tttctaaatg aacgtcgtaa ttaccgctgc
     4741 ggatagtttt cgttgcgccg acaagccgct ttagcgggtc tgaaatcgtg cgtgagaaaa
     4801 agtaaataaa aagaaacgcc actgcgatcg tcaatagcgc aattaaaatt gtcaagaccc
     4861 gcgcccaacg cacacccgcc aacgccacgt cttcgttcac ggttgtgatg agcgcaccgc
     4921 ccaacgttaa tttacgatag ctgccatatt catgcgtatc gtcgatggta taacgctgca
     4981 cgccggtgac gacttgcttt gcaaaatttt tgaacgccac gtcgacgatg gggtgttttg
     5041 aaatatcttg gccgaactta aaatgacggt tttcggaatg cgcgacaagt ttcttttttt
     5101 catcgacgag catcgccgca taaagcgaag tggccgcccc tttcgatttc accgccgaca
     5161 agagtgtcga taaactatcg ccgttcataa tggcagcaat tgcccgttct ccttttgggg
     5221 ttgccggtac gacgtaaagc cacacatgtt ggcggggcga agtcaacgca cggatgagag
     5281 ttttgccttc gcggatttct ttttgatccg cttcgctaaa tttaagatgc gccacgagct
     5341 ctgagcgcgt taaattaaag cggctcaatt tttcagcgcg ggtataaacc gctgacgctt
     5401 cttctaaaag cgaggggaag tcggcgataa gaaaaaaatc gctctcgttc gagaaatatg
     5461 atctttgaac cgcttcgctt ttacgcgaaa gcgtcgtgag caaccgcagt tgctccgcag
     5521 tggtgcgcag ctcgccctcg actttatcgg tgagcatttt ggcgttattg ccgttgttga
     5581 gcttcaccat ttcagtcata ctcgactgaa acatgttgag cgatatgccg gtaaagagca
     5641 gcacgctaat aatgatgata aacgaaacaa tcgcgatgag cttcgatcgg attgtcgtga
     5701 aaatcgatgc gtaaagcgcg cgtagtttag tgagcataaa tggcctcttt agtattcaca
     5761 gcaatatccg cagcgccctt tgtagcgttg aggcgcgcat cgaactcggg acgaaatttt
     5821 ttgataatgg g
//
LOCUS       DAOMPD010000123         5773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5884_length_5773_cov_2.561210, whole genome shotgun sequence.
ACCESSION   DAOMPD010000123 DAOMPD010000000
VERSION     DAOMPD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5884_length_5773_cov_2.561210"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLY93_05570"
     CDS             <1..372
                     /locus_tag="PLY93_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRP68982.1"
                     /translation="YFIHAQDITGSYFVFNAGAAWHYGTTKTTVKSLPIQYLGTKSVV
                     LTLRGTTIEPDGKALPNSEIQIFAKGDDAPTKTVQTDAAAKYTVSIEPGLEYKIVVKH
                     EGYAKTTTNLPLLSNDKKNNG"
     gene            365..739
                     /locus_tag="PLY93_05575"
     CDS             365..739
                     /locus_tag="PLY93_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP68983.1"
                     /translation="MDKDFILAPLVFSLEGVNFKPDSDQFVNRAAEKSILELVKFLQA
                     NSDVRVEISGHTAASGPDDANSRNLSKKRAQAVVNFMVKKGIDAKRLIAEGYGGSRPI
                     ADGKTTAGAAKNRRVEVRILTE"
     gene            871..2691
                     /locus_tag="PLY93_05580"
     CDS             871..2691
                     /locus_tag="PLY93_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP68984.1"
                     /translation="MSIRGLWDFRPYGTNAPWQKLRFEKEWRLQGIQNTAVGEYRLTI
                     HIPEHLHEENFALLLPPLSAAVQIYFNGALAYEKGSVSTVNRYPQNSSEAFFWYPIKK
                     ELLRAGIPQELRVVITGFQGGGGAFGNSFAYFGGEKEIQEKFNFLLAVTVFLAASIFM
                     IAVFHFALARDKNYRRANLHYVVLSLAMSCHIAGMNGLGYYLFDNFIFNAALIHTLFA
                     VFPFAVVGFTLRYFRLNFPRLRRSVHYFAVLMILFLTMLIAFPSLIPVYMNYVLPVAV
                     VAMALSLLFAVYGVYKGIKKKIEGAALILFGFFVYGVGVLNDVAFYFFYSTPYKLADF
                     GFLVVVVCIAIALANRLQSAAQEKEELRDWKKEITLAAQIQHQALSQRRLATEYLQIE
                     TLFKPMKIIGGDFFAFHEISNNCTGIFLADVSGHGIAAALMVNTLQNVFLQQRDCAHD
                     PSLLMHNMNKILYPQLNEQFVTAAYCFIDSGTNQMRFAQAGHPPIYLLQNNSTHLEKV
                     KPKGRFLGFDKQIEYETEVRELKRYSRVFIYSDGIIEAGSQRGTPYTVNRLEKFLQLS
                     TALKGDSLLSALESDVQTVTHTALNTDDDSSCVVVDLKVA"
     gene            complement(2698..3432)
                     /locus_tag="PLY93_05585"
     CDS             complement(2698..3432)
                     /locus_tag="PLY93_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRP68985.1"
                     /translation="MSFETTTPRSLWQRYVAVVLYLLASFLLTQAISLIALPFLSHAD
                     PLEGNRGLPFSFLLNAVVIQMAGFLIPAPLLIKFTRASYFNFHRTSIKEIFLCIGLVF
                     VSMIFFSVLYSILSIEPQQMGFLDGDDILKNKAAFVFVTSLAVPFYEEWIFRGLIFGV
                     LVTGVTERKKIIVGALFCATLFTLSHIEGKHSLSALPPIFTMALIFQYMTWRTQSLWP
                     SIAAHAFQNLLSSAAFFTKFVADTMK"
     gene            complement(3429..3830)
                     /gene="queF"
                     /locus_tag="PLY93_05590"
     CDS             complement(3429..3830)
                     /gene="queF"
                     /locus_tag="PLY93_05590"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRP68986.1"
                     /translation="MQQNEAGSYEGRQNHIRTLDLPAIEVFENIYSDKEYNIHLDFPE
                     FTAICPKTGLPDFGIVTIHYSPDKYCLELKSLKEYFLAYRNVGIFHENVVNRIVDDIV
                     AAAQMRRAKVSVVYGARGGIVTTVSRRYRRA"
     gene            complement(3838..5577)
                     /locus_tag="PLY93_05595"
     CDS             complement(3838..5577)
                     /locus_tag="PLY93_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP68987.1"
                     /translation="MIQENYYDNNADLLFNIDHFIDWATLVPLKEEGFADGANFKKTG
                     DKNLEFAPNTVEEALDGYKAVWQQTGEIAGIEVAGAAQAMDKAGLKFANGKVTFPPDM
                     LRLVDLFSQSGLLGYSMHRHHGGLSMNLTASSVVMELVSRADAAFGIALGCFNLAEVI
                     ERYASKAMSDEWVPKMAAGEVTGAMALTEPNYGSDLSRVMTRAEKSEDGTWRLNGTKR
                     FITHGCGLGDRPSVILTLARSGGAGAKGLSFFLVHSSNIQVARIEEKMGLHISPTCEI
                     IFENSKAELIGEEGLGLVRYALGMMNGARMGIAVQSLGIAHAAHAEGVKYASERVQFG
                     ATIDKLAAVKRIIDDNEAYVQGMRALVYRACAIVDHFDGLSNKLIKEGADERSVRKNE
                     EVMRLDKLAKLLTPTAKLFCSEYANFVAYHSLQIFGGSGYTEEYDIAKIYRDARIATI
                     YEGTTQLQAVAAIGGIVEGLREGGFLLTHLRAEIDRLTDATRKKGLLADVEALAKTVP
                     QYKERANKEAISIDMVTAFAHVYVSILLAEQLQIAETKNLSEIAASKASVFPYFHQLS
                     RRYLAGLQVTVAA"
BASE COUNT         1473 a         1324 c         1427 g         1549 t
ORIGIN      
        1 tattttatcc acgctcaaga catcaccggg tcttattttg tttttaacgc aggggcggcg
       61 tggcattatg gcacgacgaa gacgacggtt aaatcgctac cgattcaata cttggggaca
      121 aagagcgtcg tgttgacttt gcgcggcacg actatagagc ccgatggaaa agcgcttccc
      181 aactctgaaa ttcaaatttt tgctaaaggc gacgatgcac cgacaaaaac tgtccaaacg
      241 gatgctgcgg caaaatatac agtgtcgatt gaacccggtt tagaatacaa aattgttgtg
      301 aagcatgaag gctatgcaaa aaccacgaca aatttaccgc tcttatcgaa cgataaaaaa
      361 aacaatggat aaagatttta tcctcgcgcc gttggtcttt tctctcgagg gcgttaattt
      421 caagcccgac tcagaccagt ttgttaatcg tgcggccgaa aagtcgattc tggagctggt
      481 taagttttta caagcaaaca gcgacgtgcg cgttgaaatt agcggccaca ccgccgcatc
      541 tgggccagac gatgcgaact cgcgcaacct gtcgaagaaa cgtgcgcaag cggtggtgaa
      601 ttttatggtg aaaaagggca tcgacgcaaa acgactcatc gccgaaggtt atggcggttc
      661 gcgccccatc gcggacggta aaacaactgc aggggcggcg aaaaatcggc gtgttgaagt
      721 gcgtatcttg acagagtaga gggcgtcatc aatcgaatcc atcaactacg gcggagttta
      781 attttttgca ccgtcggatg gattcttcta cagcaatcta tttttgcgtc ggtacgcgat
      841 gcacagattt ctatccacga caaaactgtc gtgagcatcc gtggcctttg ggactttcgc
      901 ccttatggca caaatgcgcc gtggcaaaaa ttacgttttg agaaagaatg gcggttgcag
      961 ggcattcaaa acactgcggt gggcgagtac cgcctcacca ttcatattcc agagcatttg
     1021 cacgaagaaa attttgcgtt gctcttgcct cctctttcgg cagcggtgca aatttatttt
     1081 aacggtgctt tagcgtatga aaaagggagc gtcagtactg ttaatagata cccgcaaaat
     1141 tcttcagaag catttttttg gtatccgata aaaaaagaac tcctgcgcgc gggtatacca
     1201 caagaattgc gtgtcgttat cactggcttt caaggtggcg gcggcgcctt tggaaatagt
     1261 ttcgcttatt ttgggggtga aaaagaaatt caagaaaaat ttaatttttt gttagcggtg
     1321 actgtttttt tagcggcgtc tatttttatg atcgccgtgt ttcattttgc actagcgcgc
     1381 gacaaaaatt accgccgtgc caatttacat tatgtggtgc tgtcgctcgc gatgtcgtgt
     1441 cacatcgccg gtatgaatgg tttgggttat tatctttttg ataattttat ttttaacgcg
     1501 gcgctcatcc acaccttgtt tgccgtcttt ccgttcgcgg tcgtaggctt tacgctgcgc
     1561 tattttcgat tgaactttcc acgcttgcga cgcagcgtcc actatttcgc cgttttaatg
     1621 attctttttc tcacgatgct tattgctttt ccgtcgttga taccggtgta tatgaattac
     1681 gttctccccg ttgctgttgt ggcgatggcg ttgtcgctcc tttttgcggt gtacggcgtc
     1741 tataaaggga taaagaaaaa aatcgaaggt gcggcgctca tcctatttgg tttttttgtt
     1801 tatggcgtcg gtgtattgaa cgatgtcgct ttttattttt tttattcaac cccttataaa
     1861 cttgctgatt ttggttttct tgtcgttgtc gtttgtattg ccattgcgtt agccaatcgt
     1921 ttgcaaagcg ctgcccaaga aaaagaagag ctgcgcgatt ggaaaaaaga aattacttta
     1981 gccgcacaaa ttcaacatca agcgctttcg caacgcagac ttgcaaccga gtatctacaa
     2041 atagaaactc tttttaagcc gatgaaaatt atcggcggag acttctttgc tttccatgaa
     2101 atttcgaata actgcacggg gattttttta gccgatgtct cgggacacgg catcgccgcc
     2161 gcgttgatgg tcaacacgct acaaaatgtt ttcttacaac aacgggattg tgcacacgac
     2221 ccctcgctgc tcatgcacaa catgaataaa attttgtatc cgcaactcaa cgagcaattt
     2281 gtcacggcgg cgtactgctt tatcgatagc ggcacgaatc aaatgcgttt cgcccaagcg
     2341 gggcatccgc ctatttattt acttcaaaat aattcaacgc atttagaaaa agtaaaaccc
     2401 aaggggcgtt ttttgggttt cgataaacaa attgaatatg aaaccgaggt gcgtgaattg
     2461 aaacggtatt cgcgtgtgtt catttattcc gatggcatca tcgaagcggg ttcgcagcgc
     2521 ggcacccctt acacggtaaa ccgtttagaa aaatttttgc agctatccac tgcgctcaag
     2581 ggggactcac tcttgagcgc gctcgaaagc gacgtgcaaa cggtaacgca cactgcgctc
     2641 aacaccgacg acgattccag ttgcgtggtg gtggatctta aggtcgctta gcgtagatta
     2701 tttcattgtg tcggcgacaa attttgtaaa aaaggctgcc gatgaaagta aattctggaa
     2761 tgcgtgtgcg gcaatcgacg gccaaaggct ttgtgtgcgc cacgtcatgt actgaaaaat
     2821 aagagccatc gtaaaaatag gtggaagcgc gctgaggctg tgtttgcctt cgatgtggct
     2881 tagcgtaaag agggttgcgc aaaaaagagc gcctacgata attttttttc gttcggtaac
     2941 tcctgtcacc aaaacaccga aaataagtcc gcgaaaaatc cattcttcgt aaaaaggcac
     3001 ggcgagcgaa gtcacaaaga caaacgcggc tttgtttttt aaaatatcgt caccgtctaa
     3061 aaaacccatt tgctgcggtt cgatacttag gatgctgtaa agcacgctga aaaaaatcat
     3121 gcttacgaat acaagaccaa tacaaagaaa aatttcttta atcgaggtac ggtgaaaatt
     3181 aaaatacgag gcgcgtgtaa atttaatgag tagcggggca gggattaaaa aacctgccat
     3241 ttgaatcact accgcgttta agagaaaaga aaaaggtaat ccacgatttc cttcaagtgg
     3301 gtcggcgtgc gataaaaatg gcaatgcgat aagcgatatc gcttgcgtga gtaaaaaact
     3361 cgctaagaga tagagaacga ccgcaacgta gcgctgccag agtgagcgag gcgtggttgt
     3421 ctcgaaactc atgcgcgacg atagcgccgc gaaacggttg tcacaatccc tccacgtgca
     3481 ccgtagacga cgctcacttt agcgcggcgc atttgtgccg cagcgacaat gtcgtcgacg
     3541 atacggttga cgacattctc gtggaatata cccacgttac ggtatgctaa aaaatactct
     3601 ttgagagatt ttaattcaag acagtattta tccggcgagt agtgtattgt cacaataccg
     3661 aagtcgggca atccggtttt agggcaaatc gccgtgaact cgggaaaatc caaatggatg
     3721 ttatattctt tgtcggaata aatattttca aacacttcga tagcggggag gtcaagggtg
     3781 cggatatggt tttgccgtcc ctcgtaacta cccgcttcgt tttgttgcat ggttttgtta
     3841 agcggcgact gtcacctgaa ggccagccaa ataacggcgg ctgagttggt gaaagtatgg
     3901 gaacacactt gctttcgagg ctgcaatttc actgaggttt tttgtctctg caatttgcaa
     3961 ctgctcggcc aagagaatcg agacataaac gtgcgcgaat gctgtgacca tgtctattga
     4021 aatcgcttct ttatttgcgc gttctttata ttgaggcact gttttagcga gcgcttccac
     4081 gtccgcaagc aatccttttt tgcgggtcgc gtcggtgagg cggtcgattt ctgcacgcag
     4141 gtgcgttaag agaaaaccgc cttcgcgcaa gccttcgacg ataccaccga tggcggcgac
     4201 cgcttggagc tgtgttgtgc cttcgtaaat cgtcgcaatg cgcgcatcgc ggtaaatttt
     4261 tgcaatatcg tattcttcgg tgtagccgga gccgccaaaa atttgtaacg aatggtatgc
     4321 gacaaagttt gcatattcag agcaaaaaag ttttgcagtg ggggttaaaa gtttagcgag
     4381 cttgtccaag cgcatcacct cttcgttttt acgtacactg cgttcgtcag cgccttcttt
     4441 gataagcttg tttgaaagac cgtcgaaatg gtcgacgatg gcgcacgcac ggtaaacgag
     4501 cgcgcgcatt ccctgcacgt atgcttcgtt gtcgtcgatg atacgcttaa cagcggcgag
     4561 cttgtcgatg gttgcgccaa attgcacgcg ctccgaagcg tatttcaccc cttcggcgtg
     4621 cgcggcgtgc gcgatgccga gtgactgcac agcaattccc atacgggcgc cgttcatcat
     4681 gcccagtgcg taacgcacaa gacctaatcc ttcttcgccg atgagctcgg ctttactgtt
     4741 ttcaaaaata atttcgcaag tgggagaaat atggagcccc attttttctt caatgcgggc
     4801 aacttgaatg ttggagctat ggactaaaaa gaacgaaagg cctttcgcgc ccgcaccacc
     4861 cgaacgcgcg agagtcaaaa taacgctggg gcggtcacct aaaccacaac cgtgtgtgat
     4921 gaagcgtttt gtaccgttga gacgccaagt accgtcttct gatttttccg cgcgggtcat
     4981 cacgcgggaa aggtcggaac catagttggg ttcggtgagc gccattgcgc cggtgacttc
     5041 acctgcggcc attttaggca cccattcatc gctcatcgct ttgcttgcat agcgttcaat
     5101 cacctcggcg aggttaaagc aaccgagcgc aataccaaaa gccgcgtcgg cgcgtgagac
     5161 taattccatt acaaccgacg aagcggtgag gttcatgctg agaccgccgt ggtggcggtg
     5221 catcgaatag cctaaaaggc cggattgaga aaataaatcg acaaggcgga gcatgtcggg
     5281 ggggaacgtt actttgccgt tggcaaattt taagccggct ttatccatcg cttgggcggc
     5341 accggccact tctatacccg caatttcacc ggtttgttgc cacactgcct tatagccgtc
     5401 gagcgcctct tctaccgtgt tgggggcgaa ctcaagattt ttatcgccgg tctttttgaa
     5461 attggcgcca tcggcaaagc cttcttcttt aaggggaacg agcgtcgccc aatcgataaa
     5521 atggtcgatg ttaaaaagta aatcagcatt gttgtcgtaa taattttctt gtatcataga
     5581 ttattctcct tgaagcatag agcctgtgcg atggcggtgg agcccgcgca agccttatga
     5641 cgcgtcgatg attttcgacg tgttatgagt gtgaaacctc tcgtcaagcg ttacaggctt
     5701 ttatgagtat gcaacccaga gcctgtgcgc caactcgcac accaagccct tcttcccttc
     5761 taaaaatcaa aat
//
LOCUS       DAOMPD010000124         5759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5899_length_5759_cov_2.788920, whole genome shotgun sequence.
ACCESSION   DAOMPD010000124 DAOMPD010000000
VERSION     DAOMPD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_5759_cov_2.788920"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLY93_05600"
     CDS             <1..995
                     /locus_tag="PLY93_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRP68988.1"
                     /translation="GVARFDPNYGIEQKAKSTANLSKFVGETLAHLGGIDKTITVITP
                     AMKEGSGLAPFADRFPSRFFDTGIAEQHATAFAGALANTGLKPFLCIYSTFLQRGYDQ
                     LVQDIALMNLPVRLVIDRAGCVGQDGETHQGFYDIAFMAPLPHIKILSARDATELVRM
                     LTFAAHYNESPLAIRFPKKEYALFDIAAVVQSAAKKKYNPFTAESLASGKDVLILSEG
                     VFAAKALESRALLAKKKIAAAVLDVRSLKPLPTKQILNAVKNKKLIVTLENHGLVGGL
                     GDLTARLLTERNIKIKHLALGYPDTVVAHGSIGEIEKQYGLTAEKIAESIYHSL"
     gene            complement(1034..2458)
                     /locus_tag="PLY93_05605"
     CDS             complement(1034..2458)
                     /locus_tag="PLY93_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68989.1"
                     /translation="MVNEGLKFFNFSPEIIARMRETQEIPVNFYNANGQVLIPRKEQA
                     SGDMINKLLQHIATGVFYREDEEHRLGIKSSVKTEEGLADTKLLSEKKVAEIAEAAES
                     LLNDLKFATFGVVHSQKMNSQVNSFITDFEQQPDLMVGVINILDTVKVDSTSAASQQA
                     VKRAVVAMALKSRSMKAMISKDRGRGSEAMQPLMMSGMLAQIGKVKMNLPDTDKLTLE
                     QRAYIHKYPLLSYLMVAHEETISFEVKRLILNQKRTLPENMKGNNYPSLPWMKQTLQT
                     LAQENEKKGRTTIVGDIFRQLTAFKEFVLYEEDVNILSLANDFAELTTETENRAAHDP
                     EQAIKTIVNGSYFQYGPKVIRDFLDYISMSLSHNQKIIKNGDLLILALSMQSGKTYFE
                     VVRVLEVGRFQSRAMVQRLGIVNMQTVTEAGLHFSAFQVESFRADPRKIHINLAQDFL
                     RRIVYIVDEKLEPQLFEKLSKRIQ"
     gene            complement(2603..>3030)
                     /locus_tag="PLY93_05610"
     CDS             complement(2603..>3030)
                     /locus_tag="PLY93_05610"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769171.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRP68990.1"
                     /translation="FGEQAVLCGGISHLIQAGFETLTEAGYDPEIAYFECLHETKLII
                     DLIYEGGLEKMRYYVSDTAEYGDYVSGPRVIDSHVKQKMKEVLADIQKANGAKFAKNF
                     LADLRSGGKEFESMRKKEENHPIEKVGKKLRSMMRWLGK"
     assembly_gap    3031..3060
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3061..3626)
                     /gene="ilvC"
                     /locus_tag="PLY93_05615"
     CDS             complement(<3061..3626)
                     /gene="ilvC"
                     /locus_tag="PLY93_05615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRP68991.1"
                     /translation="MAKLYYDQDCDLAQLKNLTIAVIGYGSQGRGQAQNMLDSGLKVI
                     IGLKPTSQSVAEAKADGFEVVDVATASERADVIQILTPDQTQRDIFEKEIKPHLKAGN
                     ALVFSHGFNIHFDEIVAPKDVDVYMVAPKGPGHLVRRVYTEGGGVPCLIAVHQDATGK
                     AHARALAHASAVGGGRAGILETTFKEET"
     gene            complement(3771..4274)
                     /gene="msrA"
                     /locus_tag="PLY93_05620"
     CDS             complement(3771..4274)
                     /gene="msrA"
                     /locus_tag="PLY93_05620"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRP68992.1"
                     /translation="MKKAVLAGGCFWGLEELLRTQPGVINTECGYCGGNVVSPTDENH
                     DGHAEAVEITYDETRTSFRNLLNFFFQIHNPTTMNRQGNDIGASYRSAVFYANADERR
                     EAQEFIELVNRSQRWPKPVVTTLEPLGDEHGAAGRFWPAESYHQDYLQKNPNGYTCHR
                     VYFESYI"
     gene            complement(4277..4642)
                     /locus_tag="PLY93_05625"
     CDS             complement(4277..4642)
                     /locus_tag="PLY93_05625"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HRP68993.1"
                     /translation="MKINTLTPEEQQVILHKGTEYPGTGKYNKHYADGVYHCKQCDAA
                     LYRSEDKFDSECGWPSFDDTIEGSVKRVPDADGRRTEIVCARCNGHLGHVFEGERLTP
                     KNTRHCVNSISLTFQEKKK"
     gene            complement(4639..4992)
                     /locus_tag="PLY93_05630"
     CDS             complement(4639..4992)
                     /locus_tag="PLY93_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68994.1"
                     /translation="MIRKTILTALAAFLLCSSLHADQFAYLDAPLAQRAHALLSKEKE
                     AYFLCEPCGEKTGRVTTVHQVENRDAGYQAFREIYINGEAVDLAYTYVRRGNRWKNAA
                     LLLGEHPKGVSIETP"
     gene            5177..>5759
                     /locus_tag="PLY93_05635"
     CDS             5177..>5759
                     /locus_tag="PLY93_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP68995.1"
                     /translation="MFLFILGKIYAIGTADVLFLPWRPFNAAMGGQATHSLPIESSAA
                     TNAAYLGYGQQNSFFLGFLWGNAFTGFFLDTALFSPYGGLGIGADYFKADDQTAAVRF
                     SYGSFLSKHVGTGLTVTPRYTSAGKDQAFGFGIDPSLIIDSKWYKSMIGNDGLGIYSP
                     AFSLTTRNLAIPLGESNLLPLSAVHIGLTTGTYQ"
BASE COUNT         1399 a         1318 c         1377 g         1635 t
ORIGIN      
        1 atggtgtggc gagatttgac cccaattacg gcattgagca aaaagcaaaa agtacggcga
       61 acttaagtaa gtttgtcggc gagacgcttg cgcacttggg gggcatcgat aaaaccatta
      121 ctgtgattac ccctgcaatg aaagaaggct cgggccttgc gccttttgca gaccgttttc
      181 cgtcgcgctt ttttgacact ggtattgctg agcaacacgc cactgcgttt gcaggcgcgc
      241 tggcgaacac gggacttaaa ccgttcctgt gcatctattc aacattttta caacgcggtt
      301 acgaccaact ggtgcaagac atcgcactca tgaacttacc ggttaggctt gtcatcgacc
      361 gcgcgggttg cgtgggtcag gatggagaaa cgcaccaagg cttttacgat attgcgttta
      421 tggcgccgct gccgcatata aaaattttat ccgcgcgcga cgctacagaa ctggtgcgta
      481 tgcttacttt tgccgcgcac tacaatgaaa gcccactggc gattcgcttt cctaaaaaag
      541 aatacgcgct ctttgatatt gccgcagtcg tccaaagcgc ggcaaagaaa aaatataatc
      601 cctttaccgc cgaatctctg gcgtcgggaa aagatgtgct tattttaagc gaaggtgttt
      661 ttgccgccaa agcgttggag tcacgagcgc ttttagcgaa gaaaaaaatt gccgccgctg
      721 tgctcgatgt gcgttcattg aaaccgttgc cgacaaaaca aatcttgaat gccgtaaaaa
      781 ataaaaaatt gatagttact cttgagaatc acggccttgt cggcggtttg ggcgatttaa
      841 ccgcacgact tttaaccgag aggaacataa aaataaaaca cttagcgttg ggttatcctg
      901 acacggtggt cgcacacggc agcattggtg agatcgaaaa gcaatacggc ctcaccgccg
      961 agaaaattgc cgagtcgatt taccactctc tctagttatc tatccatagc ggtttacggc
     1021 tatggttaaa caatcattga atgcgctttg aaagtttttc aaaaagctgc ggttctaatt
     1081 tttcatccac gatataaaca atgcggcgca gaaaatcttg cgctaagtta atatggattt
     1141 tacgcgggtc ggcgcggaac gattccactt gaaaagcgct aaaatgtaaa cccgcttcgg
     1201 tgactgtttg catattcaca atacccagcc gctgcaccat cgcacggctt tggaagcgtc
     1261 ccacttcgag cacgcgcaca acctcgaagt aggtttttcc gctttgcatc gaaagcgcaa
     1321 gaatgagtaa gtcgccattt ttgataattt tttgattgtg gctgagcgac attgaaatgt
     1381 aatccaaaaa atcgcggatc actttggggc catactggaa ataagaaccg ttgacgatgg
     1441 ttttaatcgc ctgctcggga tcgtgtgcgg cgcggttctc ggtttcggtg gtgagttcgg
     1501 caaagtcatt tgccaaactc aagatgttta catcttcttc gtatagtacg aattctttga
     1561 acgcggtgag ttgtcgaaaa atatctccga caatggtggt gcgccccttc ttttcatttt
     1621 cttgcgccaa cgtttgcagc gtttgcttca tccaaggcag gctgggatag ttattccctt
     1681 tcatattttc aggcagcgtc cgcttttggt ttaagataag gcgcttcacc tcgaaagaaa
     1741 tcgtctcttc gtgcgccacc atgagatacg acaacagtgg gtacttgtgt atatacgcgc
     1801 gctgttcgag cgtcagctta tcggtgtcgg gtaagttcat cttgacttta ccgatttgag
     1861 cgagcatacc gctcatcatc aacggttgca ttgcctctga accgcgccct cgatctttgg
     1921 aaatcatcgc tttcatcgaa cgcgacttga gcgccatcgc tactacggca cgcttcaccg
     1981 cttgctgcga ggcggcagaa gtggagtcga ctttcaccgt gtctaaaatg ttgataaccc
     2041 ccaccatgag gtcgggctgt tgctcaaaat ctgtgatgaa cgaattgact tggctgttca
     2101 tcttttgcga atgaacgacg ccaaacgtcg caaatttcaa atcgttgagt aaactttctg
     2161 ctgcttcggc gatttcagcg actttttttt cagaaagaag tttggtgtcg gcaagtcctt
     2221 cttcggtttt taccgaggat ttaataccaa gtctatgttc ttcgtcttcg cgataaaaaa
     2281 cgcccgttgc aatatgttgc aacagtttgt tgatcatgtc ccccgacgct tgttcttttc
     2341 gtgggattaa cacttgcccg tttgcgttgt agaagttaac gggtatctct tgtgtttcgc
     2401 gcatccgcgc aataatttca ggcgagaaat taaaaaattt caaaccttcg ttaaccataa
     2461 caccgccatt ttgaaaaata gcgcataacg cgttgagctt cgtggcgatt gacgtttgct
     2521 gtgttcatga caagcgcaca gttatcgaga gagaaactcc aatatgttga atgtaggtgt
     2581 tcttgcaata aggtttaatt acttacttac ctaaccaacg catcatcgag cgcaactttt
     2641 tacccacttt ttcaatggga tggttttctt cttttttacg catcgattca aactctttcc
     2701 cgcccgaacg taaatcggct aaaaagtttt ttgcaaactt tgcaccgttg gctttttgga
     2761 tatcggccaa cacttctttc attttttgtt ttacgtggct gtcgataacg cgtgggccag
     2821 aaacgtagtc gccatactcg gctgtatcgg atacataata ccgcattttt tctaaaccgc
     2881 cttcgtagat aaggtcgatg atgagtttgg tttcgtgcaa gcattcaaag tatgcaattt
     2941 caggatcgta tcccgcttcg gtgagtgtct caaaaccagc ttgaatgaga tggctaatac
     3001 ccccacacaa tacggcttgt tcgccgaata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 tctgtttctt ctttaaaagt agtttcgaga atacctgcgc gtccaccgcc gacggcgctg
     3121 gcgtgtgcta atgcgcgtgc gtgtgctttg ccggtggcgt cttgatgaac tgcaatgagg
     3181 cagggcactc caccgccttc ggtgtaaact cggcgcacta aatggcctgg ccctttggga
     3241 gcaaccatgt aaacgtcgac atctttggga gcgacaattt cgtcgaagtg gatgttaaaa
     3301 ccgtgtgaga aaaccaacgc gttacctgct ttcaggtgag gtttaatttc tttttcgaaa
     3361 atatcgcgtt gcgtttggtc gggggtaaga atttggatga catcggcgcg ttcggatgca
     3421 gtggcgacat cgaccacttc aaaaccgtcg gcttttgctt cggccaccga ttgcgaggtg
     3481 ggtttaaggc cgataatcac tttaaggccg gagtcgagca tgttttgcgc ttggccgcgg
     3541 ccttgcgaac cgtatccaat cactgcaatc gttaagtttt taagttgtgc taaatcacaa
     3601 tcttgatcat aatagagttt tgccatacga tagagtcgac gaaagagaca gagcgtcaag
     3661 gtagttagac tcttaggagc ctgtgcgcca actcgcgcag cgtgtataaa aatttgcaaa
     3721 aaaattgcct cagcgatgag acaagagcct aaagagggaa gagtttgtat ttagatataa
     3781 ctctcaaaat acacccgatg gcaggtgtag ccattcgggt tcttttgtag atagtcctgg
     3841 tggtaacttt ccgccggcca aaatcttccg gcagcgccgt gttcatcgcc caaaggttcc
     3901 aatgttgtca ccaccggttt tggccaacgc tgtgagcggt tgacgagttc gataaactct
     3961 tgcgcttcgc ggcgttcatc ggcgttggca taaaaaactg ccgagcggta cgacgcaccg
     4021 atgtcgttac cttgtcgatt catcgtggtg gggttgtgga tttgaaaaaa gaaattcaaa
     4081 agattacgaa aactggtacg cgtttcatcg tatgtaattt ctactgcctc ggcatggccg
     4141 tcgtggtttt catcagtggg acttaccaca ttaccgccgc aatagccgca ttcggtgttg
     4201 atcaccccgg gttgcgtacg caataattct tcgagtcccc aaaaacatcc accggcgagt
     4261 actgcttttt tcataattat tttttcttct cttgaaaagt tagggagatt gagttcacac
     4321 aatggcgcgt gtttttggga gtaagtcgtt ctccctcaaa aacatgcccc aagtggccat
     4381 tgcaacgcgc gcacacaatt tcggtacgcc taccgtcggc gtcggggacg cgtttaaccg
     4441 aaccttcgat ggtgtcgtca aagcttggcc atccacactc cgaatcgaat ttatcctcag
     4501 agcggtagag cgcggcgtcg cactgtttgc aatggtacac accatccgcg tagtgcttgt
     4561 tgtacttacc ggtaccaggg tattctgttc ctttatggag gatgacttgt tgttcctcgg
     4621 gggtgagcgt gttgatcttc atggtgtttc tatacttact cctttgggat gctcgcctaa
     4681 aagaagcgcg gcgtttttcc agcgattgcc acggcggaca tacgtgtacg ctaaatcgac
     4741 agcttcgcca ttgatataaa tttcccggaa tgcttgatat cccgcgtctc tattttcaac
     4801 ctgatgcact gtagttactc gacctgtctt ttcaccgcaa ggttcgcaca gaaagtacgc
     4861 ttctttctcc ttactcaaaa gagcatgcgc acgttgagca aggggagcgt ctaaataagc
     4921 gaactggtct gcatgcagac tgctgcagag aaggaatgca gccagcgctg taagaatagt
     4981 ttttcttatc ataaattact cgacttaaag aagcgcttat gcgtcaactg taaagaagct
     5041 tattggtagg ctctaaggtt gattaaactt ggatattggg cgaagattag tttatgaaag
     5101 ttaagatatt tactttgccc cacataatga aattcaaaac gcgagagagt ctgtgacaaa
     5161 tttaacggat tgtattttgt tccttttcat tttgggaaaa atttacgcaa taggcacagc
     5221 cgatgtactt tttttacctt ggcgtccctt taatgcggcg atgggcggtc aggcaacgca
     5281 tagtttgcca atcgaatcga gcgctgccac aaacgccgcc tacttaggct atggccaaca
     5341 gaactcattt ttcttaggtt ttctttgggg caatgcattc acgggatttt ttttagatac
     5401 cgctctcttt agtccttatg gtggcttggg tatcggcgcc gattatttta aggcggacga
     5461 ccagacggcg gcggttcgtt tctcgtatgg aagttttctc tcaaaacatg ttggcacagg
     5521 attgaccgta acgcctcggt ataccagcgc cggtaaagat caagcgtttg gttttggtat
     5581 cgacccttcg ttgattatag atagtaaatg gtataaatcg atgattggca acgatggttt
     5641 gggtatttat tctcctgcgt tttcgttgac aacgcggaac ttagcgatac ctttggggga
     5701 gagcaacctc ttacctttaa gcgcggttca cattgggtta acgacgggga cttatcaaa
//
LOCUS       DAOMPD010000125         5741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5922_length_5741_cov_3.136827, whole genome shotgun sequence.
ACCESSION   DAOMPD010000125 DAOMPD010000000
VERSION     DAOMPD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5922_length_5741_cov_3.136827"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLY93_05640"
     CDS             complement(<1..857)
                     /locus_tag="PLY93_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRP68996.1"
                     /translation="MKIKIALAGNPNAGKTTVFNALCGARQRTGNFAGVTVEKKTGTL
                     KANPEIEILDVPGTYSLKAKSTDETIAANVLTGNYTGETKPDIILVIIDASNLKRSLY
                     LVGQLQELGIPMLGAITMTDIAARRGLTLDIKKLEHALGFPLCEATKATHNQIVALTQ
                     QIVTSASSATLPQSSTRKNRTKKTTLSQFTNDANEMEARYRAIDAILKKATTRALRPP
                     TSITARIDRVLTHRLVGLLAFAVIIGSVFFSIYAGAQPMMDGIDGTMKSLGSGVRSNL
                     QSYPITASL"
     gene            complement(854..1102)
                     /locus_tag="PLY93_05645"
     CDS             complement(854..1102)
                     /locus_tag="PLY93_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRP68997.1"
                     /translation="MPHPKGETPLLERSLSTLALGESGVVVRTPTQNERLAEIGLIKG
                     EKVTLLQVAPLGDPIVLQVLDTPIILRRSDLAEVIVRT"
     gene            complement(1412..1777)
                     /locus_tag="PLY93_05650"
     CDS             complement(1412..1777)
                     /locus_tag="PLY93_05650"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRP68998.1"
                     /translation="MNITVLRAKLHRAEITDANLHYEGSLTLDEDFLDASGILPNEKV
                     QIVNINNGARLETYIIQGKRGSKEVCLNGAAARLGHKGDKIIVIAYAQMPLEEAKTFQ
                     PKIVLLEDGNKIRKIMPVA"
     gene            complement(1777..2778)
                     /gene="plsX"
                     /locus_tag="PLY93_05655"
     CDS             complement(1777..2778)
                     /gene="plsX"
                     /locus_tag="PLY93_05655"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002760628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRP68999.1"
                     /translation="MWMAVDAMSGDHGAVPLVQGAVAAAREMDAHIILVGDEKIITAE
                     IQRQGYKGENIRVVHTKEIITMHESPAMAVRQKKDASVCVAAKLVAQGEALGFFSPGN
                     TGATMAAALAHVGRIDGVKRPAIATPIPREDGLATVLLDSGANVDCKVEYLTQFAIMG
                     EIYSREIMGVINPKIGLISNGEEDTKGNEQTLAAIHRLRKLPYDFVGNVEGRDLFGTG
                     KPVHVVVCDGFVGNIMLKTMEGLAKTIFSTLRKNINASAFAQGGAILMKPAFLAVKNR
                     MDYATYGGAPLLGINGVCIIGHGSSSARAAQNAIRVGLEFGQKNVIARIAENIKRFS"
     gene            3237..3923
                     /locus_tag="PLY93_05660"
     CDS             3237..3923
                     /locus_tag="PLY93_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP69000.1"
                     /translation="MKQNTGQKSRKNKSVVAEDVFAFETLPEGQRRILEAAVDEFAER
                     GYSATSTFSIAQRAKVSEALIFKYFKTKAALLKKAIFPVLVSALIPLAIRGMRGVAES
                     KHPTFALFLGELIRERLAFAKKHSRQLRVLLQELPLNDELRKSITEILSTKVLPMIRE
                     QLVFYQKNGSLVNKDADAVLNYFMPQIVTFVVGRAVFGFFSDVDEEKAIQNHVDFLIN
                     GLVGASAKKP"
     gene            3953..>4549
                     /locus_tag="PLY93_05665"
     CDS             3953..>4549
                     /locus_tag="PLY93_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRP69001.1"
                     /translation="MQQQQNFIQKLVKKINTIKLNRRTVVILISVLPILALFFFVMFR
                     SGPLAPVSVTVGVVESKSIQPAVFGVGTVEASQLFKIGPNQPGRVKRVLVDVGERVRK
                     GQLLAVMERPDLDILTPNDPEQDYVKSALKQAEQDAEERLKRAKATLDNLQNKTDANE
                     EDIANAKSELQEAQREIKRLKSGGSILQVSANQKLTAPI"
     assembly_gap    4550..4559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4560..5124
                     /locus_tag="PLY93_05670"
     CDS             <4560..5124
                     /locus_tag="PLY93_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRP69002.1"
                     /translation="GIVVARNAEPGTAVQLGQSVIDIVDPQSVRIGARFDQTGSAGLM
                     QGLKAKISLRSQNESIAGRVLRIEPLADAVTEEIMAKVVFDKQPQPLPSLGEMAEIRI
                     SLSPLPVAPTILNASLVRHEGSLGVWRLDTNNRIAFVPVKVGTSDLDGWVQVREGLNV
                     GDRIVVHSHAPLSEKSRLRIVKAIKGT"
     gene            5127..>5741
                     /locus_tag="PLY93_05675"
     CDS             5127..>5741
                     /locus_tag="PLY93_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP69003.1"
                     /translation="MISLAGRDIAYAWGKFIFTGAGLGLLIGVTLTMAGVYRGMVDDA
                     KTLLDNSGADLWVVQKDTLGPYAEPSSIYDDAYRSVKTVPGVARAANVTYLNMQVRAG
                     KKEVRVMVVGVVGGLPGEPGQPGYLIQGRYITRSHYEAVVDAATGFALGDVLTIRRNR
                     YTVVGITRRMVSSGGDPMVFIPIKDAQEAQFLKDNDSIHEQRRRT"
BASE COUNT         1391 a         1355 c         1456 g         1529 t
ORIGIN      
        1 atgagcgatg ccgttatggg ataactctgg aggttagagc gcacccccga gcccaacgat
       61 ttcatcgtgc cgtcgatgcc atccatcatc ggttgcgcac ccgcgtagat gctaaagaat
      121 acactaccaa taatcaccgc aaatgccaaa agccccacaa ggcggtgcgt caagacgcgg
      181 tcgatacgcg cggtgatcga cgtggggggg cgcaatgcac gcgtggtcgc ttttttgagt
      241 atcgcgtcga tggcgcggta gcgtgcttcc atttcattgg cgtcgttggt gaattgagag
      301 agtgttgttt ttttagtacg attttttctt gtacttgatt gcggaagagt ggctgaagat
      361 gcactcgtca caatttgttg ggtgagtgcg acaatttgat tatgagtggc tttcgtggct
      421 tcgcacaggg gaaatcccaa tgcgtgctcg agttttttga tgtcgagagt caatccgcgc
      481 cgcgccgcga tgtcggtcat cgtgattgca cccagcatcg ggatacctaa ctcttggagc
      541 tggccgacta gatagagcga acgcttgagg ttcgaggcgt cgatgatcac caaaattata
      601 tcgggtttgg tttcacccgt gtaattaccg gtgagtacgt tcgcggcgat agtttcgtcg
      661 gtcgattttg ctttgaggct gtacgtcccc ggtacgtcga gaatttcaat ttcaggattt
      721 gcttttaagg taccggtttt tttttcaacg gtgacgcccg caaagttacc ggtgcgttgc
      781 cgcgcgccgc aaagcgcgtt gaataccgtc gttttacccg cgttagggtt gccggcgagt
      841 gcaattttta ttttcatgtg cgaactatta cctcggctaa atcggaacgg cgcaggataa
      901 tcggcgtatc caatacctgg agcacaatag ggtctcccaa aggcgccact tggagcagcg
      961 tcaccttctc tcctttgatg agaccaattt cggcgaggcg ctcgttttga gtgggtgtac
     1021 gcaccacaac gccgctttcg ccgagcgcaa gtgtactcaa tgaacgttct aaaagtgggg
     1081 tttccccttt tgggtgcggc atgttatgga attccagagc taatatgtat tttattgcat
     1141 gacgtgtgag tgtatcgctt gagtaattta cccatgcgca ttccgttttg cgcgtgacac
     1201 taacgattgg ttgcactgct cgcaggagga cttcactttg ccccgaaagc gtaggtagag
     1261 atagcctgct gccacaaaga gaatgagaat aactatcaag tagtccattg ttggggaata
     1321 tatacgattt gagtcagcac ggcaactctt tgggctttgt ttccgttgct ttttaggagg
     1381 ctgtgcgcca actcgtgcag cttcctctcg cttaagccac aggcataatc ttacgtattt
     1441 tattgccgtc ttcgagcaac acaattttgg gttggaatgt ttttgcttct tccaaaggca
     1501 tttgcgcgta agcgatgaca ataattttat cgcctttatg tcccaagcgc gcagcagcgc
     1561 cgttgagaca gacttctttt gaaccacgct ttccttggat gatgtacgtt tcgagccttg
     1621 cgccgttatt gatattcaca atttgtactt tttcgttggg caaaatcccc gatgcgtcga
     1681 gaaaatcctc gtcgagagtg agactgcctt cgtagtgtaa attggcgtcg gtgatttctg
     1741 ctcggtgcag tttcgctcgt aaaaccgtta tattcattaa ctaaacctct ttatgttttc
     1801 agcaatacgg gcaatgacat ttttttgacc aaattctaac cccacacgga tggcgttttg
     1861 tgcagcccgt gcactcgacg aaccatgccc aatgatgcaa accccgttaa tccccaaaag
     1921 aggggcgccg ccatacgtgg cgtaatccat gcgatttttt accgctagaa atgccggctt
     1981 cataaggatt gctccgcctt gtgcaaacgc cgatgcgttg atatttttcc taagagtaga
     2041 aaaaatagtt ttcgccaagc cctccattgt tttgagcatg atgttaccca caaaaccgtc
     2101 gcacaccacg acgtgcaccg gcttgcctgt gccgaaaaga tcgcgtcctt cgacgttacc
     2161 cacaaaatcg taaggcaatt tgcgtagacg gtggattgct gcaagagttt gctcgttgcc
     2221 ctttgtgtct tcttcgccgt ttgagatgag gccaattttg gggttgatta cccccataat
     2281 ttcgcgcgag taaatctcgc ccatgatagc aaactgcgtc aagtactcga ctttgcaatc
     2341 gacgtttgcg ccggaatcaa gaagcactgt cgccaagccg tcttcgcgcg ggatgggcgt
     2401 tgcaatcgca ggacgtttta cgccgtcgat gcgccccaca tgcgcgagcg cagccgccat
     2461 tgttgctcct gtgttgccgg gggagaaaaa acctaacgcc tcgccttgcg cgacaagctt
     2521 tgctgccacg cataccgacg cgtctttctt ttggcgcact gccattgcag ggctttcgtg
     2581 cattgtgata atttcttttg tgtgaacgac acggatgttc tcgcctttat aaccttgccg
     2641 ttggatttca gcggtgataa ttttttcgtc accgactaaa ataatatgcg catccatttc
     2701 gcgtgcagcg gctacagccc cttggactaa aggcaccgcg ccgtgatcac cactcatggc
     2761 atcgactgcc atccacatat ttgcttaaac cgtgctttta gtcacgctgc caagctctta
     2821 atggatttga gaaatctttt cgatatcgct taagtttatc gtgtcggtgg cgcatgccct
     2881 ttcgcaaagc tcagggtcgt ggcttatgcc aaagcaaaaa ccacaaacag cgcaccggat
     2941 ttcaacgatg tgagggcggc gggttgcgcc gaatgattgt aaaatacggt cgtgtctttt
     3001 gtagcgtgcg cgcgcgtacg ttttagcgtt aaagatgttg cggttgcatt ggcaccgtag
     3061 attcaaaaca ctcaactttt cttcgcgcat aattcatcta aaataaaatg ataaaagcgt
     3121 acgtcattcg tgatttatct ctgcaacaaa aatttagaat atttttaagc caacaaaacc
     3181 tcttaaaaaa gtttcttttt cttgacgagt gagtgagtac ttactatatt gcttctatga
     3241 agcaaaatac cggtcaaaaa tcaagaaaaa ataaatccgt cgtcgctgaa gatgtgtttg
     3301 cgtttgaaac attacccgaa ggccagcggc gcattttaga agcagcggtc gatgagttcg
     3361 ccgaacgagg gtacagtgcg acttcgacgt tttcgattgc tcaacgcgcc aaagtttcgg
     3421 aggcgcttat ttttaaatat tttaaaacaa aagcggcgct tcttaaaaaa gcaatttttc
     3481 ctgtgttggt gagtgcgctc atcccgctgg cgattcgggg catgcggggt gttgccgaaa
     3541 gcaagcaccc cactttcgcc ctattcttgg gagagcttat tagggagcgt ttggcgtttg
     3601 ctaaaaagca tagccgccaa ttacgggtgc ttttgcaaga gctccccttg aacgacgagt
     3661 tgcgtaaaag catcacagaa attctttcaa cgaaagtatt gccgatgatc cgcgagcagc
     3721 ttgtgttcta tcaaaagaac ggttcgttgg ttaacaaaga tgccgatgcg gttttgaatt
     3781 attttatgcc acaaattgtg acattcgtag tggggcgtgc tgtgtttggt tttttctccg
     3841 acgtggacga agaaaaagca atccaaaacc acgtggactt tctgatcaat ggtttagtgg
     3901 gcgcatcggc taagaaacct tgactttcaa aaaataaata gaggaaaaac aaatgcaaca
     3961 gcaacaaaat tttattcaaa agctcgtaaa gaaaataaac acaattaagc tcaaccggcg
     4021 caccgtggtg attttaattt cagtgctgcc tatcttggct cttttcttct ttgtcatgtt
     4081 tcgttctggg cctttagccc cggtgtcggt gacagtaggc gtggttgaat ccaaatcgat
     4141 tcaacccgca gtttttggcg tgggtacggt tgaggcgagc caacttttca aaattggccc
     4201 caaccaaccc gggcgcgtca agcgggtgct cgtcgatgtg ggtgagcgtg tgcgcaaagg
     4261 gcagcttctc gcggttatgg aacgccccga cctggatatt ttgacaccca acgatcccga
     4321 acaagattac gtaaagagcg cgctgaagca agccgaacaa gatgccgaag agcgtcttaa
     4381 acgcgcaaaa gccactttgg ataatctcca aaataaaaca gatgcaaatg aagaagatat
     4441 tgcaaatgca aagagcgaat tgcaagaagc acagcgtgaa atcaaacgtc ttaaaagtgg
     4501 cggctctata ttacaagtga gtgcaaacca aaagctgacc gctcctattn nnnnnnnnnt
     4561 ggcattgtcg ttgcgcgtaa cgccgaacct ggtaccgcag ttcaattagg acaatcggtc
     4621 attgatattg tcgatccgca aagtgttcgc attggtgcac gctttgacca aacaggctcg
     4681 gcggggctta tgcaagggct caaagctaaa atttcattgc gttcgcaaaa cgaaagtatc
     4741 gcgggtcggg tgttgcgtat cgagccttta gccgacgcgg tgaccgaaga gattatggcg
     4801 aaagttgttt ttgacaaaca accgcaacca ttgccttctt tgggtgaaat ggcagaaatc
     4861 agaatttcac tttctccttt gccagtggcg cccacaattt tgaacgccag cttggtgcgc
     4921 cacgaaggct ctctgggtgt gtggaggctc gatacaaata accgcatcgc ttttgtaccc
     4981 gttaaagttg gcacaagcga cctcgacggt tgggttcagg tgcgcgaagg actcaacgtt
     5041 ggtgatcgta tcgttgtgca ttcgcacgca ccgctttcgg aaaaaagccg gctgcggatt
     5101 gtgaaagcca tcaaaggaac ataaccatga ttagtcttgc aggacgcgac attgcgtacg
     5161 cgtggggaaa atttattttc accggagcgg ggttgggact ccttattgga gtgacgctca
     5221 cgatggccgg tgtttatcgc ggtatggtcg atgacgctaa aacactttta gacaacagtg
     5281 gcgcggatct ttgggtggta caaaaagata cacttggtcc ttacgccgaa ccttcgagca
     5341 tttacgacga tgcgtaccgc agtgtaaaaa ccgtgcccgg tgtcgcgcgc gcggcgaatg
     5401 ttacctacct caacatgcaa gttcgtgccg gtaaaaaaga agtgcgcgtg atggtcgttg
     5461 gcgtagtggg tggtttaccc ggtgaacccg gacaacccgg gtatttaatc caaggccgat
     5521 acatcacccg ttcgcactac gaagcagtgg tcgatgcagc caccggtttt gcgttgggag
     5581 atgttctcac aatacggcgc aaccgctata ccgtcgtcgg gatcacccgt cgtatggttt
     5641 cgtcgggtgg cgatccgatg gtttttattc ctataaaaga tgcgcaagaa gcgcagttct
     5701 taaaagacaa cgactcgata cacgaacaac gacgacgcac c
//
LOCUS       DAOMPD010000126         5726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5941_length_5726_cov_2.940928, whole genome shotgun sequence.
ACCESSION   DAOMPD010000126 DAOMPD010000000
VERSION     DAOMPD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_5726_cov_2.940928"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLY93_05680"
     CDS             complement(<1..691)
                     /locus_tag="PLY93_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRP69004.1"
                     /translation="MNLVHRILLCLFSVFCLSSVFATESAPPLILTDAQGRYSLAEHI
                     SILTDPSGKFTLEQILENKNTEPFIANKDANPNFGYTAAAHWVRFSAINQSSSKSQFY
                     VEIAYPMLDFVDFYQVRDKKVVQHQRAGDRLPFYTREVQYHEITFPMTLELNRTDDIY
                     MRVQSTSSVQLPLVLWSSTAYTSYVSQQRTFLGLYYGMMLVMVFYNFFLFISVRDKNY
                     LYYVIFILGYLF"
     gene            837..1859
                     /locus_tag="PLY93_05685"
     CDS             837..1859
                     /locus_tag="PLY93_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRP69005.1"
                     /translation="MQIVYLTDIHDNFAGVSSVLNSTEADLYILSGDLTYHCFGTDDA
                     LFRFIELQDKIRTLALKAGAAVSDIHATNRFAEKAKQGEIPTLLSAREGEEYLTLAQE
                     AHQELLRKYQTMHKAIVATRKKCFIIPGNYDIELSATAVSSYNIHKKSEIVDSFKFAG
                     YGSAPIFTPGIPQELTVPFEESDVGTTLKSQPMDFMLAEKADVFILHNPPYGTLDKLA
                     RYGHCGSQGLREAVDKVNPRLVLSGHVHESYGLLKLGSTYFLNPSNFGSVESVDGPQF
                     GGYFATLQLQHDETGQKYLREVVWKRLVDRRIHELCRVKIDKNQRAAETVIDQAEFQK
                     MERFLL"
     gene            1863..2534
                     /locus_tag="PLY93_05690"
     CDS             1863..2534
                     /locus_tag="PLY93_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69006.1"
                     /translation="MRNPFPENETFKKFLGIKDYFRKFTTQENMLRTRELARAARIMQ
                     QIGTPISFDLLGSVNFGMAVEASDVDIVMYIECDHDEEARYDNTPMLRIYESLFFATI
                     LKEISHNPFQLQIVDFINLRRLSRAIYEPGFDDDILARFVFYRTICRGVNKLPIRKYE
                     QAISGNKELFHRIEDHLTEALKEFTRSSSHRMSFNKYLSRLRDREIKIPASMVEKIHE
                     YLDKT"
     gene            complement(2579..3064)
                     /locus_tag="PLY93_05695"
     CDS             complement(2579..3064)
                     /locus_tag="PLY93_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRP69007.1"
                     /translation="MRSLRPLFDRHSPADALEREAERLKNRDWVEFLRVVHPTQSQIA
                     EREYILSRIASDWQTLTTPILATHTLPVDWQHEKLLVACDHNTFASEIALLAPTIEKQ
                     IVARYGIRLKIHSRAVKRIDWEKPTAPTSAISINPTKKQPPPKHENAAVLEELIRRLS
                     N"
     gene            complement(3068..>5726)
                     /locus_tag="PLY93_05700"
     CDS             complement(3068..>5726)
                     /locus_tag="PLY93_05700"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRP69008.1"
                     /translation="KKEILHTAEEMPTSAAELTALFTPQDDEKKAATQSHYPDSKIVN
                     KTRTPHKKRRRWLYAAMALVILGIASFVLLRVRLKKTLVPEEGAAVVQGITANVTISR
                     DDLGVPHIKAENLDDMAFGAGFAMAADRNFQLEFLRRMAYGRLSEVLGKDTLGLDIYM
                     RSLGFGRLATQNYTNLDTKMRQMLKAFAAGVNAERKAFPKREFEFMLLRLSPEEWQPQ
                     DSLALYQLFSFLLATNSVEEMAFLKLAAKLGPEKAAWLFPIYDDEPLPFEEVNQLKGV
                     DWQKFKNIDATKKAFLDRVNPTLALNTLLPEPSLPASNNWAIAPARTKNGKTLIANDT
                     HLQMTVPAMWYMMNLECPEYRAAGVALPGTPIVALGTNGTLAWGATMVMGDNQDIFLE
                     QLREKNGKTQYLYKGKWLDAKITHEKIQVKGENTYNFKRTETEHGVLLNEALSHAMPD
                     KYLAVRLESEYGIAYSTTVDEEESTLRGFYNMAKARTMKEARTALDKVTGIYLNIVYG
                     DEKNIGWLATGRYPARTGKTGQLPRIGWTGDNDWDGYYLPSENPSKVNPPEGFIATAN
                     HRVWGPDAETDLWISASWYSADRADRAKELIRKSGKLTAEDRTRIQADTVSLTAKRIK
                     VLLFSPGFHGELQAAVNKLPALQKKQAEEALAFFQNFDGNLDKNSAAAAVYENFADAV
                     AKLAFADELSGQDSSWWITFQVANKRSYNAVHDHLLGRSDSPFWDNLQTPEKETKATI
                     FAQALAAAIAANENSMGTNRNDWAWGKIHQYYWKHQFTQKAGFLKGYLNRGPIPAGGD
                     MQTLNQAGNLWGDSHNVWLIPAMRFIIDFSKQEPASLIVHMGISGNPESPHYADMIPL
                     FTDVKNWPLPLEPIQALKQYSRHFVLTKE"
BASE COUNT         1435 a         1351 c         1411 g         1529 t
ORIGIN      
        1 aaaacaaata gcctaaaata aaaataacgt agtacaaata atttttgtcg cgcaccgaaa
       61 taaaaagaaa aaagttgtag aacaccatca cgagcatcat gccgtaatag agccccaaga
      121 aagtgcgctg ttgcgaaaca tagctggtgt acgcagtcga gctccacaaa acaagcggca
      181 actgcaccga actggtcgac tgcacgcgca tataaatatc gtctgttcgg ttgagttcaa
      241 gcgtcatcgg aaacgttatt tcgtggtatt gtacttcgcg cgtataaaac ggcagacgat
      301 cgcctgcccg ctggtgttga accactttct tgtcacgcac ttggtagaag tcgacaaagt
      361 ccagcatggg gtaggcaatc tctacataaa attgcgactt tgaactggat tgattgattg
      421 ccgaaaaacg tacccagtgg gcggcagcgg tgtacccgaa attgggattg gcgtctttat
      481 ttgcaatgaa tggttctgta tttttatttt caagaatttg ttcgagagtg aattttcccg
      541 aagggtcggt taaaatgcta atgtgttctg caagcgagta acgcccttgc gcatcggtga
      601 ggatgagagg cggcgcactc tctgtggcaa agacagagga aaggcaaaat accgaaaaaa
      661 gacaaagtaa aattcgatgg actaaattca tgaaagcttt attttttata ccataccatt
      721 aatgtgtgac atcaagtaaa agctaaaggg tgaatgggtt tctgaaaact tgaaataatc
      781 gcattaaact tcacgattgt cgtactgact cccgctaaaa aagcgacaag aagcagatgc
      841 aaattgtcta cctcaccgat atccatgata attttgctgg cgtgtcgagc gtcttaaact
      901 caaccgaagc cgatctctat attctttcgg gcgatttaac ataccattgt tttggtaccg
      961 acgacgccct ctttcgtttt atcgaactgc aagataaaat acgcactctg gcgctcaaag
     1021 ccggcgcggc ggtgagcgat atacacgcca ccaatcgctt tgctgaaaaa gcaaaacaag
     1081 gcgagatccc cacactctta tccgcccgtg aaggtgaaga atatttaacg ctcgctcaag
     1141 aagcgcacca agagctgctg cgcaaatacc aaacgatgca caaagccatc gtcgcaacgc
     1201 gcaaaaaatg ttttattatt cccggtaact acgacatcga gctttctgcc acggcggtgt
     1261 cttcgtacaa catccataaa aaaagcgaaa ttgtggattc ctttaagttt gccggttatg
     1321 gcagcgcgcc gattttcacg ccggggattc cgcaagaatt gactgtcccg tttgaagaat
     1381 cggatgttgg cacaacgctc aaaagtcaac cgatggattt catgctcgcc gaaaaagccg
     1441 atgtctttat tttgcataat cctccttatg gcactttgga taaattggcg cggtacggcc
     1501 attgtggttc gcaaggtttg cgcgaagccg tcgataaagt gaatccccgc ttggtgcttt
     1561 cggggcatgt gcatgaaagt tatggattat taaaattggg atcgacatac tttttgaacc
     1621 cgtctaattt tggtagcgtc gaaagcgtcg atggcccaca gttcggcggc tatttcgcca
     1681 cgctacaact ccaacacgac gagacgggac aaaagtattt gcgcgaggtg gtgtggaagc
     1741 gtttggtcga tcggcgcatc cacgaactct gccgcgttaa aatcgacaag aaccaacgcg
     1801 ccgcagaaac ggtgattgat caggcggagt ttcaaaaaat ggagagattt cttttgtaac
     1861 gcatgcgaaa ccctttcccc gaaaacgaga cgttcaaaaa atttttaggt attaaagatt
     1921 attttcgtaa attcaccacg caggaaaata tgctgcgtac tcgcgagttg gcgcgcgcgg
     1981 cgcggatcat gcagcaaatc ggtacaccca tttcgttcga tcttttgggt tcggtgaatt
     2041 ttggtatggc ggtcgaagct tccgatgtag acattgtgat gtatatcgaa tgcgaccacg
     2101 acgaagaagc gcgctacgac aacacgccga tgctgcgcat ttacgaatcg ttattttttg
     2161 cgacgatact taaagaaatt agccataacc catttcagtt gcagatcgtc gactttatca
     2221 atttacgccg cttgagccgc gcgatctacg aacccggttt tgacgacgac attttagcgc
     2281 gttttgtttt ttaccgcacc atctgccgtg gcgtcaacaa attgcctatc cgcaaatacg
     2341 aacaggccat ctctggaaat aaagaactct tccaccgcat cgaagaccac ctcaccgagg
     2401 cgttgaagga attcacccgc tcgtcgagcc atcgcatgtc gttcaataaa tacctctcac
     2461 ggctacgtga ccgcgaaata aaaatcccgg catcgatggt cgagaagatt cacgaatatt
     2521 tagacaagac ttaactatag ctttttattt tgaaaggaaa gcttgcaaaa taaaaaaatt
     2581 aattcgataa acgacggatg agctcttcca acactgcggc attttcgtgt ttgggaggtg
     2641 gttgtttttt agtcggattt atactgatcg cgctcgtggg tgcggtgggt ttttcccagt
     2701 cgatgcgctt tactgcgcgg ctatggattt tgagacgaat accgtagcgc gccacgattt
     2761 gcttttcaat cgtcggcgcc agcaacgcaa tctctgaggc aaatgtattg tggtcgcacg
     2821 ccaccaacag tttctcatgc tgccaatcga cgggcagtgt gtgcgttgcc agaattggcg
     2881 ttgtgagtgt ttgccaatcg ctggcgatac gcgagaggat gtattcgcgc tcggcgattt
     2941 gagattgtgt ggggtgtacc acgcgcaaga attctaccca gtcgcggttt ttgagccgct
     3001 cggcctcgcg ttccaacgca tcggcaggac tatgccgatc gaaaagagga cgcaatgaac
     3061 gcatcgtcta ctctttcgtg agcacaaaat gccgcgagta ctgcttgagc gcctggatcg
     3121 gctccaaggg tagtggccag tttttcacat cggtaaaaag cggtatcatg tcggcgtaat
     3181 gcggcgattc aggattgcct gaaatcccca tgtgaacgat gagcgaagcc ggctcttgtt
     3241 ttgagaaatc gataataaag cgcattgcag ggattaacca tacattgtgc gaatcccccc
     3301 ataagtttcc cgcttggttg agtgtctgca tgtcgccacc ggcgggtatg gggcctcggt
     3361 tcaagtaacc tttgaggaag cccgcctttt gcgtgaattg gtgtttccaa tagtattggt
     3421 ggattttacc ccatgcccaa tcgttgcggt tcgtgcccat tgagttttcg tttgcggcaa
     3481 tagcggcggc gagtgcctgt gcgaatatag ttgctttcgt ttctttttcg ggtgtctgga
     3541 ggttgtccca aaaaggactg tcgctgcggc ccaatagatg gtcgtgaact gcgttgtacg
     3601 aacgcttgtt agcgacttga aaagtaatcc accacgacga atcttgccca ctcagttcat
     3661 cggcaaaagc aagtttagcc acagcatcgg caaaattttc atacacggcg gcagcggcgg
     3721 agttcttatc gagattgccg tcgaaatttt gaaaaaaggc gagtgcttct tctgcttgct
     3781 ttttttgtaa tgcaggtaat ttattcaccg ccgcttgaag ctcgccgtga aaacctggtg
     3841 agaacaacaa tactttgatc cgctttgccg ttagtgaaac ggtgtctgct tggatgcgcg
     3901 tcctatcttc agcggtgagt ttgccacttt ttctaataag ttcttttgcg cggtcggcac
     3961 ggtcggcgct gtaccatgat gcagagatcc acaaatcggt ttcagcgtcc ggcccccaca
     4021 cacgatggtt tgccgttgcg ataaagcctt caggaggatt caccttcgat gggttttctg
     4081 aaggcaagta gtaaccatcc caatcgttgt cgcctgtcca accgatacgg ggtaactgtc
     4141 ccgttttgcc agtgcgagcg gggtaacgac cggtggccag ccaaccgata tttttttcat
     4201 caccgtagac gatattgaga tagattccgg tcactttatc gagtgcggta cgcgcttctt
     4261 tcatggtgcg tgcttttgcc atattataaa aaccgcgcaa tgtcgattct tcttcgtcta
     4321 ccgttgtgct gtacgcaata ccgtattcgc tctccaaacg taccgccagg tatttatcgg
     4381 gcattgcgtg cgaaagcgct tcgttcaaaa gaacaccgtg ctccgtttca gtgcgtttga
     4441 agttgtatgt attctcaccc ttgacttgaa ttttctcatg cgttattttt gcatcgagcc
     4501 atttaccctt atagaggtat tgtgttttgc cgttcttttc acgcagttgc tccagaaaaa
     4561 tatcttggtt atcgcccatc accatcgttg caccccatgc gagcgtgccg ttagtgccca
     4621 acgcgacaat cggggtgccg ggaagcgcaa cccctgccgc acgatattca gggcattcta
     4681 agttcatcat gtaccacatc gccggtacag tcatttgcag gtgtgtgtcg ttagcaatca
     4741 atgtcttgcc atttttagtg cgcgcgggag caatcgccca gttattcgag gcggggagcg
     4801 acggttctgg taataacgtg tttaatgcca aagtgggatt taccctatcg aggaatgctt
     4861 tttttgtggc gtcgatattt ttaaactttt gccaatcaac tcctttgagt tgattcactt
     4921 cttcaaacgg caatggttcg tcgtcataaa tgggaaagag ccacgccgct ttttcgggtc
     4981 ccaactttgc ggcaagtttt aggaaagcca tctcctcaac gctgttggtg gctagcaaga
     5041 aggaaaaaag ttggtagagc gccaacgagt cttgcggttg ccattcttcg ggagaaaggc
     5101 gcaacagcat aaattcaaac tcgcgtttag ggaacgcttt gcgctcggcg ttcaccccgg
     5161 cggcgaacgc tttgagcatt tgtcgcattt tcgtatcgag gttcgtatag ttttgagtag
     5221 caagacgccc aaagcccagc gagcgcatat aaatatcgag tcccagcgta tctttcccca
     5281 acacctctga aagacgacca tacgccatac ggcgcaagaa ctcaagctgg aaattacggt
     5341 cggcggccat tgcgaagcct gcaccgaatg ccatgtcatc caagttctcg gctttgatat
     5401 gtggcacgcc taaatcgtcg cggctaatcg ttacgttggc ggtaatgcct tggacaaccg
     5461 ctgctccttc ttcaggcacg agggtttttt tcaaacgaac acgcaacaaa acaaaagaag
     5521 cgatacccaa aataacgagg gccattgctg cgtaaagcca gcggcggcgt ttcttatggg
     5581 gtgtgcgcgt tttattaact attttactgt cggggtaatg cgattgagta gcagcttttt
     5641 tttcgtcgtc ttgtggcgta aaaagtgcag taagttcagc cgccgaggta ggcatttctt
     5701 cagcggtgtg cagtatttct ttcttc
//
LOCUS       DAOMPD010000127         5693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5984_length_5693_cov_2.475523, whole genome shotgun sequence.
ACCESSION   DAOMPD010000127 DAOMPD010000000
VERSION     DAOMPD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5984_length_5693_cov_2.475523"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLY93_05705"
     CDS             complement(<1..967)
                     /locus_tag="PLY93_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRP69009.1"
                     /translation="MNGGEIAARVIQAHNIDAMFTLIGGHISPILVGAKKLGIRVVDT
                     RHEVNAVFAADAWARLKGKPGVAAVTAGPGVTNTVTALRNALMAESPLVLLGGATATA
                     LKGRGALQDIDQITLIRSNVKKAYRVTRVKDIAPVLTKAFHTAQEGIPGPVFVELPVD
                     LLYDEKIIREWHEEGAPKGNSLSAKFIRGYLKYHVNRLLKDNDKVKIPAPLKIHTHSI
                     SQSLVDKIRTQIENAERPLILAGSQTVLQAQKANEISNALESLGVPVYLSGMARGLLG
                     ATNALQFKQKRKDALKEADLIILLGVPADFRLNYGAHINRNAYQIN"
     gene            complement(1122..2483)
                     /locus_tag="PLY93_05710"
     CDS             complement(1122..2483)
                     /locus_tag="PLY93_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRP69010.1"
                     /translation="MANKKVTPPSLSLDTILTWKDRIKEIFYLKEDTDYGGTIDNLRK
                     GVEIRGQGVYTLIFAIFIASVGLNINSTAVIIGAMLISPLMGPIMGAGLALGTYDFLL
                     LKRAGMNLFVMTSISLLASTLYFWLSPLGDAQSELLARTSPTIYDVLIATFGGATGIF
                     AASRRDKASNAIPGVAIATALMPPLCTAGFGLATGNFRFFVGALYLYLINSIFIGLST
                     FIFIRAVGFRAVGKIRATVDKRIRRYVLSIAILVAIPSIYMAYDIVKESKFAENVRKY
                     VAQNFNFEKSRVLNTTVRHKNGRKVLEISIVGSPISDEMITHLRAQLTGFSLEDTELV
                     IIQPEKFGLPAPSTEMNNMISETKDKNDRIHALENEIRRLRSPTRQIGLIAKEVNVVF
                     PQIESISFGDLMNENIRFMTSDTAPTLLVKWKKNTSTTEKKKFAVFIKLRLNIENIQL
                     IEL"
     gene            2594..3952
                     /locus_tag="PLY93_05715"
     CDS             2594..3952
                     /locus_tag="PLY93_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP69011.1"
                     /translation="MAESKSPLMERIKLALGISYRYARGKSKYFLSMNAFLSFVGVFV
                     GTSLLVVVMSIFGGFQAQLKKSIFQFDPHVMLSVDRITGGEKIQGWQKWKERLTAELS
                     GDVESVEGNIQSPAIFRKRKFLDHVFVRGMDFENDTKTHTFKLPSYFPEIVEPKGVRT
                     FPEGDYAIVGKEMAANLGLSVGDSIELIVPRGQFSLQTGVTPSIRSFKIAGLFKTGHY
                     TYDTKVVVLSLKTAQNLYALPDAVQQLVLRLKNPDDLAKLRKKIGRAWPYTVRTIEDE
                     QRNFFAALRLEKTIMIVIVFLFIIAAMVGIIIATFNIVRSHRRDIGIMKSLGFSRRQI
                     LVIFAVTGLAMGVLGSVSGIFAGVGLANNLESILHGIEAVINFFGNFWCAKVSQCVWL
                     DVRLIPRDVYYFDHLPIDIDWKLLRILGYVSVCLSILASIIPARKAAKLEPIDIIRKA
                     EF"
     gene            3954..4646
                     /locus_tag="PLY93_05720"
     CDS             3954..4646
                     /locus_tag="PLY93_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP69012.1"
                     /translation="MTAENIPQSILTVADLHKTYRDGEAFAEVLKGISFTVNAGEFMA
                     IQGPSGAGKSTLLNLLGALDKPDQGKIVIDGLELTSAMKNKTIELFRRDKIGFIFQNH
                     YLMQEFTVVENVMLALRVQGMATKEAKEKAVHILERIGLGHRVNHYPSQISGGESQRV
                     AVARAVVKNPPLVLADEPTGNLDSDNRARFIDILNKLQAEDKLTVIVVTHEQELAMAA
                     SRRLMMRDGLLE"
     gene            complement(5027..>5693)
                     /gene="lnt"
                     /locus_tag="PLY93_05725"
     CDS             complement(5027..>5693)
                     /gene="lnt"
                     /locus_tag="PLY93_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRP69013.1"
                     /translation="PIIINDILHDPSTRKDYSNMWHLDGQGVPLANYQKHLLLPFGEF
                     MPMGETFPTLKNLFPAVSDFSHGTKFSLFNIATRTGFMKAMPLVCYEVILPNFAQAFD
                     NKTSDAAQFIINITNDAWFGDSVESIQHLTLGVMRAIELRLPIVRATNSGISAYIASN
                     GSVHGKTRLFERVNAVYEVPSMPRSTTLFAFWGYIPLYLFLFFGVGWFAYKAYRLRTK
                     KSK"
BASE COUNT         1492 a         1178 c         1420 g         1603 t
ORIGIN      
        1 cgttgatttg gtacgcgttc cggttgatgt gtgcgccata atttaagcgg aagtcagcgg
       61 gtacgcccag gaggataatt aaatcggctt ctttgagcgc atctttccgt ttttgcttaa
      121 actgcaacgc gttggttgca cctaataaac cacgcgccat ccccgacaag tatacaggaa
      181 ctcccagtga ttccaaagca ttactaatct cgtttgcttt ttgcgcttgg agcacggttt
      241 gactgcctgc taaaatgagt ggtcgttctg cgttttcaat ttgggtgcgg attttatcga
      301 caagactttg cgaaatactg tgggtgtgaa ttttaagcgg tgcagggatt tttactttgt
      361 cgttgtcttt gagcaatcga ttgacgtggt acttcaaata cccacggata aattttgccg
      421 aaagactgtt gcctttagga gcgccttctt cgtgccactc gcggataatt ttttcatcgt
      481 agagtaaatc aacaggaagc tcgacaaaaa caggccccgg gattccttct tgcgcggtgt
      541 ggaatgcctt tgttaaaacc ggcgcaatgt ctttcacgcg ggtgacgcgg tacgcttttt
      601 ttacgttcga gcggatgagt gtaatttggt cgatgtcttg gagcgcacca cggcctttaa
      661 gcgcagtggc ggtggcgcct ccaaggagca ccaagggcga ttccgccatg agtgcgttgc
      721 gtaacgcagt gacggtgttt gtcactccag gccctgcggt caccgccgca acgcctggct
      781 ttcctttgag gcgtgcccac gcgtcggcgg caaaaaccgc attcacttcg tggcgcgtat
      841 cgacaacacg aattccgagt ttttttgctc ccactaagat aggcgagata tgcccgccga
      901 ttaaagtaaa catagcgtcg atgttgtgcg cttggataac gcgtgctgcg atttcaccac
      961 cattcataac ggcagaagag aacattctta tagcgcgtca atttgttgta gtaagttgga
     1021 tttgacagct tgattcaaaa agccagtcta cagaaacagt cttaaaaaaa agcgaaagaa
     1081 atttttaaga cacaacagca ggtctttagc atttttattt tttatagttc gataagctgg
     1141 atgttttcga tattcaatcg cagttttata aaaactgcaa atttcttttt ttcggtagta
     1201 gaagtgtttt ttttccattt aactaagaga gtcggcgctg tatcgctcgt cataaatcgt
     1261 atattttcat tcatgagatc cccgaaagat atactctcaa tttgtggaaa aacaacattc
     1321 acctctttag caatcagccc tatttgtcgg gtaggacttc ttaatctgcg tatttcgttt
     1381 tctaacgcgt gaatgcgatc gttcttatct ttagtctcgg atatcatgtt gttcatttcg
     1441 gtactcgggg caggcaagcc gaatttctct ggctgaataa tgactaactc tgtgtcttcg
     1501 agcgagaaac cagtcagttg ggcacgcaag tgggtaatca tttcgtcgga aataggcgaa
     1561 ccgacaatag aaatttcaag tacctttctc ccattcttat gccgtaccgt tgtattaaga
     1621 acgcgacttt tttcaaaatt aaaattttgc gcgacgtact tacgcacatt ttctgcaaat
     1681 ttagattctt tgacaatatc gtatgccata tagatgctgg gtatggctac aagaattgcg
     1741 atactgagta catatctgcg gatgcgttta tcaacggttg cccgaatttt gccaactgct
     1801 cgaaatccga cggcgcgtat aaaaataaaa gtcgatagac caatgaatat gctattgatt
     1861 aagtaaagat ataacgcacc gacaaaaaag cgaaaatttc cggtggccaa accgaagcct
     1921 gcagtacaca gaggaggcat gagcgctgtg gcaatggcaa cgccaggtat tgcattcgag
     1981 gctttgtctc gacgtgaagc ggcaaaaata cctgtcgcgc cgccaaaggt tgcaataagg
     2041 acgtcataaa tggtgggtga ggtgcgtgca agaagttcag attgagcgtc gcctaaaggg
     2101 gaaagccaaa aataaagagt tgaagctaaa aggctgatag aggtcattac aaataggttc
     2161 ataccagccc gtttcagaag taagaagtcg tatgtgccaa gagctaaacc tgcgcccata
     2221 atggggccca tgagtggcga gatgagcatg gcgccgatga ttactgccgt cgagtttata
     2281 tttaacccga ctgaagcgat aaaaatggca aatattaatg tgtatacgcc ttggcctcgt
     2341 atttcgactc ctttgcggag gttgtcgatt gtaccgccgt aatcggtgtc ttctttaaga
     2401 taaaatattt ctttgatgcg atctttccaa gtaaggatag tgtcgagaga gagggaaggg
     2461 ggagtgactt ttttattcgc catagccagt ggtacataac taagataact ggcaaataaa
     2521 aatataggaa gttagattta atatatctcg ccgtggtgta tcgattctaa aatcctgtta
     2581 atggaattta tagatggcag agagtaaatc ccccctcatg gaacgtataa aacttgcgtt
     2641 aggcattagt taccgctacg cacgcggcaa gagcaaatac tttctgagca tgaatgcgtt
     2701 tttatcgttt gtcggtgttt ttgtcggtac cagtctcttg gtggtggtga tgtcgatttt
     2761 cggaggtttc caagcacagc tcaaaaaaag tattttccag tttgatccgc acgttatgct
     2821 ctcggtcgac cgtatcaccg gtggcgagaa aattcagggc tggcaaaaat ggaaagaacg
     2881 cctcactgcc gagctttcag gcgatgtcga gtcggtcgaa ggcaatattc aatcgcccgc
     2941 tattttccgt aaaagaaaat ttttagatca tgtgtttgtg cgtgggatgg actttgagaa
     3001 cgacacaaaa acacatacat ttaaactgcc gtcgtacttc ccagaaattg tcgaacctaa
     3061 aggcgtgcgt actttccccg agggcgacta cgcaattgtg ggtaaagaga tggccgcaaa
     3121 tctggggtta agtgtcggcg actcgattga gcttattgtg ccgcgtggcc aatttagcct
     3181 acaaaccgga gtgactcctt cgattcgatc ttttaagatc gcagggttgt tcaaaacagg
     3241 acattatacg tacgatacaa aagtggtggt gttgtcgctc aaaaccgcac agaatttata
     3301 cgcgctcccc gatgcggtgc agcaactcgt gttgcgcctt aaaaaccccg acgatttagc
     3361 aaagctcaga aaaaaaatag gccgtgcatg gccttatact gtacgcacga ttgaggacga
     3421 acagcgcaat ttttttgcag cgttgcgtct ggaaaaaacg attatgatag tcattgtttt
     3481 tctttttatt atcgcagcga tggtgggtat tatcatcgct acgtttaaca ttgtgcgctc
     3541 gcaccggcgc gacatcggta ttatgaaatc gctcggtttt tcacgaaggc aaattttagt
     3601 gatttttgcc gtcaccggtc ttgccatggg agtgttggga tcggtttcgg gtatctttgc
     3661 aggggttggg cttgccaata acttagaatc gattttgcac ggtatcgaag cggtgattaa
     3721 cttttttgga aatttttggt gtgcgaaagt aagccaatgc gtttggctcg atgtgcggct
     3781 catccctcgc gatgtgtatt attttgacca cctacccatc gatatcgatt ggaaacttct
     3841 acgcatcctg ggttatgtct cggtgtgcct ttcgatttta gcgtcgatta tccctgcgcg
     3901 caaagcggct aaacttgaac cgattgatat cattcgtaag gcggagttct aaaatgacgg
     3961 cagaaaatat accacaatct attttaaccg tcgccgattt acataagaca taccgcgacg
     4021 gcgaggcatt cgccgaggtt ctaaaaggaa tttcttttac agtcaacgcg ggtgaattta
     4081 tggcaattca aggcccctcg ggtgcaggta aaagtacgct cttgaatctt ttgggtgcgc
     4141 tcgataaacc cgatcaaggt aagatcgtca tcgacgggct tgagcttacc agcgcgatga
     4201 aaaataaaac gattgagctg ttccgccgcg ataaaatcgg cttcattttt caaaaccact
     4261 acttgatgca agagttcaca gtggtggaaa acgtgatgct tgcgctacgg gtgcaaggta
     4321 tggctacgaa agaagccaaa gaaaaagcag tgcatatttt agagcgtatt ggcttggggc
     4381 accgcgtgaa tcattacccc tcacaaatat cgggcgggga gagccagcgc gtcgctgtgg
     4441 cacgggcagt agtaaaaaat ccgccgcttg ttttagccga tgaacccacc ggtaacttag
     4501 acagtgacaa ccgcgcacgc tttatcgata ttttgaacaa gttacaggca gaagataagt
     4561 tgacggtgat tgtggtgacg cacgagcaag aactggcgat ggccgcctcg cgtcggctca
     4621 tgatgcgcga tggtttgttg gagtagtcta tttattgaaa actgagtttt tttgagaaac
     4681 atacgcttcc aaatataagt tcattaaatt cttgtatgcc cacctgtttc actcgtcgtt
     4741 tttctaaaat aatcgatttt tcagattatg tcgcattaga ggataactat cccagttttt
     4801 tagattatgt cgcattaagg gataactgtc tcaatttttc atattatgtc acattagggg
     4861 gtaactatcc tatttctcgg attatgtcac attaggggat aactatccgc tccaagtgct
     4921 ggagtggtca aaaacaaggg tacaatttga agaaaatttt cttctatgcg tttgggttat
     4981 cgatttttgg gcgcattgag ctatactggc tgagtttggc tggtggttac ttggattttt
     5041 ttgttcgcaa tcgatatgct ttataagcga accatcccac accgaaaaat aaaaacaggt
     5101 aaagggggat atacccccag aatgcgaaga gggtggttga gcgtggcatc gatgggactt
     5161 cgtagacggc gttgactcgt tcaaaaaggc gggttttacc gtgaacgctg ccgttcgagg
     5221 cgatgtatgc gctgatgccc gagttggtcg cacgcacgat ggggaggcgt agttcaatcg
     5281 cgcgcatcac tcccaatgtc aaatgttgga tgctctcaac cgagtcgcca aaccatgcgt
     5341 cgttggtgat gttgatgatg aattgcgcag cgtctgaagt cttgttgtcg aatgcttgtg
     5401 caaaattagg taagatcact tcgtagcaga cgaggggcat cgctttcata aagccagtgc
     5461 gggtggcgat attaaaaaga gaaaattttg taccgtggga aaagtcggac accgcaggga
     5521 agagattttt gagggtaggg aaggtttcgc ccattggcat aaattccccg aacggcaaaa
     5581 gaaggtgctt ttggtaattg gcgagcggca caccttggcc gtctaaatgc cacatgttcg
     5641 agtagtcttt gcgcgtggag ggatcgtgca aaatatcgtt gatgatgatg ggt
//
LOCUS       DAOMPD010000128         5691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_5985_length_5691_cov_2.491483, whole genome shotgun sequence.
ACCESSION   DAOMPD010000128 DAOMPD010000000
VERSION     DAOMPD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5985_length_5691_cov_2.491483"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLY93_05730"
     CDS             complement(<1..349)
                     /locus_tag="PLY93_05730"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRP69014.1"
                     /translation="MGFILKRFSLPFIFIAFCAVCGKSNAAGEKTAQDPKTTFSFERP
                     FVLHDKTQKVAIFAGGCFWCMESPFEKLAGVKAVIVGYTDGKEKNPTYSEVSYGNTGH
                     VEAVLVVYNPKVVQ"
     gene            complement(361..1191)
                     /locus_tag="PLY93_05735"
     CDS             complement(361..1191)
                     /locus_tag="PLY93_05735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRP69015.1"
                     /translation="MITERKSPQNIKPHKISVSFDESLPKHWFGGNAFLTHLLNTFTA
                     IFPDAERYFVRWSKKALSNVTDSKIKKDIQGFIGQETMHASQHEKLWAILRTQGYNLE
                     PVLWIVQKFGFGWLENILSDKVNYAAVAGFEHFTAMFGEIYFENPHLMSKMHPEMRRL
                     FEWHAAEEIEHKAVAFDQLQNIDDSYLLRTSVMNGAVLLLYFVAFGNTFYFMLQDRSL
                     FEPKEWANIYELFFGKHDFARKSFFKFIEYYKPNFHPWENQNYHLAEPTLRAYEKQVS
                     "
     gene            complement(1215..>1778)
                     /locus_tag="PLY93_05740"
     CDS             complement(1215..>1778)
                     /locus_tag="PLY93_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69016.1"
                     /translation="KNDAVEKALDDLAVFRPLILAGVCKEKTMAEIYDDVSEAINDMG
                     YTNCHAKYPQGVLGHKVGKIPTGLIPRIPFMRFELSTFLYLGAEELSRFLNPWDNHTA
                     LWSDYTRVSADPGLWAVEPHIGMAHGGASVSGAKDGAKAPTMAHGGAAVFGVKWEEIL
                     HVTENDAYYLDDNLPHVNRWQERGVAI"
     assembly_gap    1779..1806
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1807..2171)
                     /locus_tag="PLY93_05745"
     CDS             complement(<1807..2171)
                     /locus_tag="PLY93_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRP69017.1"
                     /translation="MSREFYTETDIANFRECQSLAYDAVAAVQKRLKVGTTEKEAAEM
                     IDEEIKARGMTRYFHAPFAWFGERTAFKGFERPLNWGNFPKRFLPPHFGLKFLPTNTR
                     LEEGMCVILDVAPIYNDASA"
     gene            complement(2307..3107)
                     /locus_tag="PLY93_05750"
     CDS             complement(2307..3107)
                     /locus_tag="PLY93_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRP69018.1"
                     /translation="MPQAPVLNDFIQTDAFIAPGSSGGPLLNMRGEVIGINSRGGSGL
                     AFTIPIETALDVKEKLLKGGSIKRGFLGIQLMPIDLEMRKLLRISDTEGVLVTLVLKS
                     SSFLNKLKPLDVIRKVNGTPLSAVEDKDVNVIIRRMSELPIGQEAKLEVLRDGKLITV
                     VGHVKERPPAEGKKWQSTMGFVLMEISDDLADRHQLENKSGVYVSFIDNGSAAQQSSL
                     RLGDVILNVDGKSVSNIDDAKAALSIDKKEYLLQVSRARAQFYLLLKP"
     gene            complement(3086..3649)
                     /locus_tag="PLY93_05755"
     CDS             complement(3086..3649)
                     /locus_tag="PLY93_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRP69019.1"
                     /translation="MKQPHREIAQLKKMKLLHGLIPLILFGAPLIARPLPPELERLEQ
                     HIASVSEEITQSVVHIKVDGSLNNKKFSSMGSGFVINTQGMILTNHHVIDNSTKVMVK
                     FNHDAREYEAKVLGSDEATDVALIQVNAVEVKHLYPVALGKSKSTKVGQWVLAVGNPY
                     GLDRTVSFGIVSAKERSLCVTCHKRRC"
     gene            complement(3892..>4171)
                     /locus_tag="PLY93_05760"
     CDS             complement(3892..>4171)
                     /locus_tag="PLY93_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRP69020.1"
                     /translation="PDTIGIVPLGTPMVGGPALITTCVLMATEYGMVATLISLFINVL
                     IGAVVFSYGQVLTRFLGDSGSAALSKITSLLLASIAVMMVRRGVLSMV"
     assembly_gap    4172..4197
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4198..4478)
                     /locus_tag="PLY93_05765"
     CDS             complement(<4198..4478)
                     /locus_tag="PLY93_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRP69021.1"
                     /translation="MELLFKNIIITFIPIFVAMDAVGILPIYISMTRKHGPAERGRIV
                     RLSMLTAFLLTALFIVLGQSIFRFMGISVFDFMVAGGVILFIIAIQDVMG"
     gene            4586..>5691
                     /locus_tag="PLY93_05770"
     CDS             4586..>5691
                     /locus_tag="PLY93_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP69022.1"
                     /translation="MADKNTKSEIQRVFELQKKFKWSLRETTAHERREKLKKLKEKIL
                     AESENLYQALWQDYKKPREEADLAEVLPIMSELHDAIKNLPDWMRPKVVETPIHLGTG
                     RSEVHYEPLGQTLIIAPWNYPFNLSIAPIISAIAAGNTVMLKPSELAPNTAAFLERIL
                     KDLFYEEEVAVFQGDASVSTELLKLPFDHMFFTGAPNIGKIVMKAAAEHLSVCTLELG
                     GKSPAIVENTADIREAGKRIAWGKWVNAGQTCIAPDYILVSEDKKDALIDAIHDTVKS
                     FYGQTPIEWRNSPDFCRIVNRRHYDRVKGLLDDAKKHGAEIAFGGDTDEHDNYISPTV
                     ITNIADGSEILKEEIFGPLLPVVTYRHIDEALEYV"
BASE COUNT         1539 a         1429 c         1234 g         1435 t
ORIGIN      
        1 actgcaccac tttgggattg tacaccacca gcaccgcttc cacatgcccc gtgttgccat
       61 aagaaacttc gctgtaagtg gggttttttt ctttgccgtc ggtatagccg acgatgaccg
      121 ctttcacgcc cgcgagtttt tcaaagggag actccataca ccaaaaacaa ccgccggcga
      181 agatggccac tttttgagtt ttatcgtgca gcacaaaagg ccgctcaaaa ctaaacgttg
      241 ttttggggtc ttgagcggtt ttttctccgg cggcgttact tttaccacac accgcacaga
      301 aggcaataaa aataaacggt aatgaaaagc gcttcagtat gaatcccact ttaataaaat
      361 ttacgaaacc tgcttttcat acgccctgag cgtgggttca gctaaatggt agttctgatt
      421 ttcccacgga tggaaattgg gtttgtagta ttcgataaac ttgaaaaaac tcttgcgcgc
      481 gaaatcatgt ttgccgaaaa aaagttcata aatattcgcc cactctttgg gttcaaaaag
      541 cgaacgatct tgcaacatga aatagaacgt gttgccgaat gcaacaaaat acaagagcag
      601 cacagcgcca ttcataacac tggtgcgcaa taagtagcta tcgtcgatat tttgtagttg
      661 atcgaacgca accgccttgt gctcaatttc ttcggctgca tgccattcaa agagacggcg
      721 catctcgggg tgcattttac tcataaggtg tgggttttca aaatatattt cgccgaacat
      781 ggcggtaaaa tgttcaaacc cagccaccgc cgcataattt actttgtccg ataaaatgtt
      841 ttcgagccaa ccaaaaccaa atttttgcac aatccaaagc acaggctcaa gattatagcc
      901 ttgtgtacgt agaatagccc acagcttttc gtgctgactg gcgtgcatcg tttcttgtcc
      961 gataaagcct tgaatatctt ttttaatttt gctatcggta acattggaaa gcgccttttt
     1021 cgaccaacgc acaaagtatc tttcggcatc ggggaatatc gcagtaaatg tattcagcaa
     1081 atgtgtcaag aatgcattcc cgccaaacca gtgcttgggt aaactttcat caaagctcac
     1141 gctgattttg tgtggtttta tattttgagg agattttctt tctgttatca tattgtttat
     1201 ctacttctat tcgtttatat agcaactcct cgctcttgcc agcgattgac gtgcggtaaa
     1261 ttatcgtcta aatagtacgc atcattttcg gtgacgtgta aaatttcttc ccatttgacg
     1321 ccaaaaaccg cagcacctcc atgtgccatg gtcggcgcct ttgcgccatc cttggcgccg
     1381 ctgaccgagg cacctccatg tgccatccct atatgcggtt ccaccgccca cagcccgggg
     1441 tctgccgaca cccgtgtata gtccgaccac aacgcagtgt gattgtccca aggattcaaa
     1501 aaacgactca actcctcggc gcctaaatag agaaacgtcg atagctcaaa acgcatgaac
     1561 gggatgcggg gaattaaccc cgtggggatt ttacccactt tgtgccccaa cacgccttga
     1621 gggtatttcg catggcaatt ggtataaccc atgtcgttaa tcgcttcgct cacatcgtcg
     1681 taaatctcag ccatcgtttt ttctttgcaa acacccgcta aaataagagg acggaatacc
     1741 gcaaggtcat ccaacgcctt ttcaaccgca tcatttttnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnngccg aggcgtcgtt gtaaatggga gccacatcta agatgacgca cattccttct
     1861 tctaaacgcg tattcgtggg taaaaatttt aatccaaaat gtggcggtaa aaatcttttg
     1921 gggaaattac cccagttgag tggtcgctcg aaccctttga acgccgtgcg ctcgccaaac
     1981 cacgcaaacg gcgcgtggaa gtagcgtgtc atcccgcgtg ctttgatctc ttcgtcgatc
     2041 atctccgccg cttctttttc ggttgtgcca actttgagcc gcttctgaac ggctgcgacg
     2101 gcatcgtaag cgagcgattg gcattcccta aaattcgcta tatcggtttc ggtataaaat
     2161 tcgcgcgaca tgatgcacgg caataaacat gaacagaatg tcaatttatt ctaaggaatt
     2221 attcgtgatg tcgtacaaag aaaagtgtct aatggcgctg ttccattcgc tacggcgcag
     2281 tcattagaca gccccactca agagcatcac ggcttgagca acaagtaaaa ctgcgcacgc
     2341 gcacgactca cttgcaacaa atactctttt ttatctattg aaagcgcggc cttagcatcg
     2401 tcaatattcg agacgctttt gccgtcgacg ttcaaaataa catcccctaa acgcaagctc
     2461 gactgctgcg ctgcactgcc gttgtcgatg aagctcacat acacacccga tttattttcg
     2521 agttgatgtc tatcggctaa atcgtctgaa atttccatga gcacaaaacc catggtcgat
     2581 tgccatttct taccttcggc cggtgggcgc tctttgacat gccctactac ggtgataagt
     2641 ttgccatcgc gcaacacttc caacttcgct tcctgaccaa tcggtagttc gctcatgcgt
     2701 cggatgatca cgttcacatc tttatcttcc accgcgctta aaggcgtacc gttgactttg
     2761 cgaatcacgt ctaacggttt gagcttattt aagaaactcg acgatttgag caccaacgtc
     2821 acaagcacac cctctgtatc ggagatacga aggagtttac gcatttctaa atcgatcggc
     2881 atgagttgta ttcccaagaa accacgtttg atacttcccc ctttcaaaag tttttctttt
     2941 acatcgagcg cggtctcaat ggggatggta aacgctaaac cagaaccgcc acgagagtta
     3001 atcccaatga cttcaccgcg catgttgagg agcggacctc ccgacgaccc cggagcgata
     3061 aacgcgtctg tttggataaa gtcgttcagc accggcgctt gtggcacgtt acgcaaagcg
     3121 aacgctcctt cgccgacaca atgccaaagc tcaccgtccg atcgaggcca tagggattac
     3181 ccactgcgag gacccactgc cctactttcg tactcttact tttacccaat gccacggggt
     3241 ataagtgttt cacctcgacg gcattgactt ggatgagcgc aacatcggtt gcttcgtccg
     3301 aacctaaaac tttcgcttcg tattcgcgcg cgtcgtggtt gaatttcacc atgaccttgg
     3361 tgctattgtc gatgacgtgg tggttcgtca aaatcatgcc ttgcgtattg atgacaaaac
     3421 cagaccccat gctgctgaat tttttattat tgaggctacc atcgacttta atatgcacca
     3481 cactttgagt gatttcttcg ctgacactcg cgatgtgctg ttcgagtctt tcaagctcgg
     3541 ggggaagtgg gcgcgctatc aaaggagcac cgaaaagaat cagtgggatt aacccatgca
     3601 gtagtttcat tttttttaat tgagcgattt cacgatgcgg ttgtttcacg attgtgtact
     3661 cgattcgtta taggaatcgt tttcgttatc gctcatagtt attctcccag tagttaatca
     3721 aaaaagttgt agggattatc caaaaaatca aaaaatttat atttggggga gtactccctc
     3781 aatcaatttt gactttttag gttggtcaat agccgctcag cggcttgtta gacatccccc
     3841 gcaaatctca agacgccaga cgatacggta tattgattgg gtcaagagat tttacaccat
     3901 actcaacacg ccacgacgca ccatcatcac cgcaatcgaa gctaaaagta aactcgtaat
     3961 cttagataac gccgccgagc ccgagtctcc caaaaaccga gtgagcactt ggccatacga
     4021 aaacaccacc gcaccgatta agacgttaat aaaaagagag atgagcgttg caaccatacc
     4081 gtattccgtc gccatgagaa cgcacgtagt gataagcgca gggccgccca ccattggcgt
     4141 gcctaaagga acaatcccaa tggtgtcggg gnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     4201 atcacgtctt ggattgcaat gatgaaaaga atcactcccc ccgccaccat aaagtcaaac
     4261 acggagatac ccatgaatcg aaatatcgac tgccctaata caatgaaaag cgcggtgagt
     4321 aagaatgcgg tgagcatcga cagccgcaca atacgccctc tttccgcagg gccgtgcttg
     4381 cgcgtcatgc tgatatagat cggtaaaata cccaccgcgt ccatggcgac gaaaataggg
     4441 ataaaggtaa taataatatt tttgaataat aactccacaa acccaactaa aaaaaaccgt
     4501 tacggtggcg agataaaagg aataaacaga tcacaataac tgtaaaaaac cttgccaccc
     4561 ccgcctgaat tgctaatcga gacttatggc cgacaaaaac acaaaatctg aaatacagcg
     4621 cgtctttgaa ctccagaaaa aatttaagtg gtcgttgcgc gaaaccaccg cgcacgaacg
     4681 ccgtgagaaa ttaaaaaagc tcaaagaaaa aattttagca gagagcgaaa atttatacca
     4741 agcgctgtgg caagattaca aaaagccgcg cgaagaggcc gacctggctg aagtactgcc
     4801 aattatgtcg gaacttcacg atgcgattaa aaatctcccc gattggatgc gccccaaagt
     4861 ggtagagaca ccgatacacc tgggaacagg ccgcagcgaa gtgcattacg aaccgctggg
     4921 gcaaacgctc atcattgcgc cgtggaacta tccttttaat ttaagcattg ctccaattat
     4981 tagcgcgatt gctgccggaa atacagtgat gcttaaacct tccgaactgg cccccaacac
     5041 cgccgcattt ttagaacgca tcttgaaaga tttattttac gaagaagaag tggcggtgtt
     5101 ccaaggcgac gcgtcggttt ccaccgaact cttgaaactc ccattcgacc acatgttttt
     5161 taccggcgcg cccaatatcg gtaaaattgt gatgaaagca gccgcagaac acctttcggt
     5221 gtgtacgctt gagttgggcg gtaaatcccc cgccattgtg gaaaacaccg ccgacatccg
     5281 cgaagcgggc aagcggattg cttggggtaa atgggtgaat gctggtcaaa cgtgtatcgc
     5341 gcccgattac atcttggttt ccgaggacaa aaaagacgcg ctcatcgacg ccatccacga
     5401 caccgtaaaa tcattctatg gccaaacacc cattgaatgg cgtaattctc ccgacttttg
     5461 ccgcatcgtc aaccgccgcc actacgaccg tgttaagggt ctgctcgacg acgcgaagaa
     5521 gcacggtgcg gaaattgcat tcggcggcga taccgacgag cacgacaact atatttctcc
     5581 gacagtgatt actaatatcg ccgatggaag tgaaattctt aaagaagaaa tttttggtcc
     5641 actgttgccg gtggtgacat accgccatat cgacgaagca ttggagtatg t
//
LOCUS       DAOMPD010000129         5648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6044_length_5648_cov_3.225282, whole genome shotgun sequence.
ACCESSION   DAOMPD010000129 DAOMPD010000000
VERSION     DAOMPD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6044_length_5648_cov_3.225282"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PLY93_05775"
     CDS             complement(<1..1344)
                     /locus_tag="PLY93_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69023.1"
                     /translation="MDDLISLLLIGGIVGVIGIFAYYVVRTYLIPKRVEELAEMIRQG
                     HVGPAISKLNKMIEENDRDPYLHFLLGEAYSKQNDLQAAATEYKQVIKIGKWNPQVKE
                     VSVRSRLAKIYMQNRNYDEAKKEYLILTRLDGTNHENYYQAGILFETAGLVDKALPYF
                     KQSAKLKPTHADSFLHIGTAEYHFGNLSDAKLALTEAVKLNSNLHSAHYYLGMCLKNQ
                     KDYDWAVKEFDLAAKDENLKGRALLAKGLSLMEKGQLPTAQIELEKGLLVAPKGGDLE
                     LNIRYAIAACAERKRDFHTAISNWERIIDVNAKFRDVQDKLKAYDEFRTDDTIKDFMI
                     APPGKFEAQTRAIVEAMTYKVVDLTVIDDSEVHILATEEEGNRRTSKKSNRLIYILRT
                     TDLVPERIVRTLHEEMRSKGANKGVCISTSDFSSQAHAFIQSRPIELYDRKQLIAL"
     gene            complement(1347..1730)
                     /locus_tag="PLY93_05780"
     CDS             complement(1347..1730)
                     /locus_tag="PLY93_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69024.1"
                     /translation="MDLKEIEALKSENASLRQTLRGYETVVRLNDEEINQLEQMIAMY
                     EQIIEFSRAELIEAREEQKANEQLSTLTRDELITAKQTVEASESVSKMSREELMHALA
                     QIKELEVENKRLKEQRLAVGEKLNS"
     gene            1873..2172
                     /locus_tag="PLY93_05785"
     CDS             1873..2172
                     /locus_tag="PLY93_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69025.1"
                     /translation="MEQQIQQIVNFAIGTVRGLGDAAKNTLTSLEAEVKSLVDKGAAA
                     SDENAQKIRANATDLVNRVTNIVNTAVNNLNDVTGQAQKLVQDAIAKVKPESKQG"
     gene            complement(2219..3508)
                     /locus_tag="PLY93_05790"
     CDS             complement(2219..3508)
                     /locus_tag="PLY93_05790"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRP69026.1"
                     /translation="MEHTAKLIYDGNEYELPVIHGTENELAIDIAKLRAQTGLVTLDD
                     GYVNTGSTMSSITFLDGEKGILRYRGYDIAELAENSTFLEVAYLLIYGNLPNAQVFEN
                     FKQTITRHTLIHEDLKRLYDGFPKDAHPMATLSSMINTRSGYYIKDASDPLDPIGREL
                     SINRLLAKTPTIVAFSYKKSIGQPMMYPKNSLSYIANFIRMLFAVPAENYTADPVVER
                     ALDLLLILHADHEQNCSTSTVRLVGSSRANVFAAISSGVNALWGPLHGGANQAVIEML
                     EQIHKEGGDVKKFVTLAKDKNSNFRLMGFGHRVYKNFDPRAKIIKKACMDVLAKLGVK
                     DPLLDIALQLEEAALKDDYFVERKLYPNVDFYSGLIYRAIGIPTEMFTVMFALGRMPG
                     WIAQWKEMIENPAAKIGRPRQIYNGEKARPFVPLIKR"
     gene            3640..4836
                     /locus_tag="PLY93_05795"
     CDS             3640..4836
                     /locus_tag="PLY93_05795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRP69027.1"
                     /translation="MPDLIAEINALEKRAAEQIAAAADANALQKTTQEFIGKKGVLTV
                     VMKQLGTLSNEERGAVGKAANAFKVKLEELLAAKEASLAEAKYASLAETEFIDLSFTP
                     PEFAAVQTGSAYSGHVHPIAQTQKKLEDIFTSMGFSIVDGPQIEDDYHNFGALNFTDD
                     HPARDMQDTFYVTHGGAQQATNTTAYLLRTHTSSVQIRALEKMQPPLRIIAPGRVFRY
                     EEVDASHEHTFYQMEGMIIDRHISVGNLLHLMQTLLTEVFGRKVEVRLRPGYFPFVEP
                     GFELDMKCLVCESADSREDDIRGGATPVTSVSTGSHTDGLHASASPVTSVSLGCSVCK
                     YSGWVEVLPCGLVHPNVLRNIGVNPSAWQGAAFGLGLNRLVMMQHGIEDIRHFMSGKL
                     SFLRQF"
     gene            4846..5310
                     /gene="fliW"
                     /locus_tag="PLY93_05800"
     CDS             4846..5310
                     /gene="fliW"
                     /locus_tag="PLY93_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRP69028.1"
                     /translation="MTFETRALGRIEIEASKQLFFPEGLFAFEAFRHFALLPTKSGST
                     FHWLQSLEETRLAFLVTPITEITRRYKPSIQEQYLAVIEAQTIASLEIWGIITVPAGK
                     PENMTINLQGPILINREKKLGSQFVSDNTEHLVRTPLLDLVDEDKKNAEGNS"
     gene            5310..5537
                     /gene="csrA"
                     /locus_tag="PLY93_05805"
     CDS             5310..5537
                     /gene="csrA"
                     /locus_tag="PLY93_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRP69029.1"
                     /translation="MLVLARRVNDSIVIGDSVEIVVVEIKGDQVKLGVKAPKDVKVFR
                     GEIHAEIQKENKAALESQLPPDLDTLLSKKK"
BASE COUNT         1499 a         1292 c         1272 g         1585 t
ORIGIN      
        1 aagcgcgatg agttgcttgc ggtcgtaaag ttcaattggg cgcgattgga tgaacgcatg
       61 cgcttggctt gaaaaatcag acgtgctaat acacacccct ttattagcgc ctttggatcg
      121 catttcttcg tgtagagtgc gcacaatgcg ttcgggtact aaatcggttg tgcgtaaaat
      181 ataaatgagt cgatttgatt ttttacttgt gcgccgattg ccttcttctt cggttgccaa
      241 aatatgcact tcagaatcgt cgataacggt taaatcgacg actttatacg tcatggcttc
      301 gacgatggcg cgggtctgtg cttcaaattt ccctggagga gcaatcataa aatctttgat
      361 cgtgtcgtcg gtacggaatt cgtcataggc tttcaattta tcttgcacgt cacggaattt
      421 tgcattcacg tcaatgatgc gttcccagtt cgagatagca gtatggaaat cgcgcttgcg
      481 ttcggcgcaa gcggcaatgg cgtaacgtat gtttagctct aaatctcctc ctttgggtgc
      541 aactaaaaga cctttttcta gttcaatctg agccgtcggt aactgccctt tctccatgag
      601 cgagagtcct ttggcgagca aagcgcgtcc ttttagattt tcatcttttg cagctaagtc
      661 gaattccttt accgcccaat cgtaatcttt ttgattttta aggcacattc ctaaataata
      721 atgcgcgcta tgcaggttgc tgttcaattt caccgcttcg gtaagagcaa gttttgcatc
      781 gcttaaattg ccgaaatgat attccgctgt cccgatatgc aaaaaggaat cggcatgggt
      841 gggttttagt ttagccgatt gtttaaagta gggtagcgct ttatcgacaa gaccggcagt
      901 ttcaaacaat atacccgctt gataataatt ttcatgattt gtaccgtcta accgagtcaa
      961 aattaaatat tcttttttgg cttcgtcgta attgcggttt tgcatataaa tttttgctaa
     1021 cctgctacgc acggatactt cttttacttg tggattccac ttgccgattt taataacttg
     1081 tttatattca gttgcggcag cttgcaagtc gttttgtttt gaatacgctt cgccgagcaa
     1141 aaaatgcaaa taaggatcgc gatcgttctc ttcaatcatt ttgttgagct tcgatattgc
     1201 tggtcccacg tgtccttgtc gaatcatttc ggcaagttct tccacgcgtt tgggaattaa
     1261 atacgtacgc acaacatagt atgcaaagat gccgataaca ccgacaatac cgccgatgag
     1321 taaaaggcta attaaatcgt ccattgtcag gaattcagtt tttcaccaac tgctaaccgt
     1381 tgttctttca accgtttgtt ttcgacttct agttctttaa tttgagctaa cgcatgcatc
     1441 aactcttccc ggctcatttt agatacgctt tcagaagctt caacggtttg ctttgctgtt
     1501 atcagttcat cgcgtgtcaa cgtcgacaac tgttcgtttg ctttttgctc ttcacgtgct
     1561 tcaatcaatt ccgcgcggct aaattcgata atctgctcat acatagcaat catctgttcg
     1621 agctgattta tctcttcgtc gttcaaacgt acaactgttt cgtaaccgcg cagggtttgc
     1681 cgcaacgagg cgttttcgct tttgagcgct tctatctcct tcaaatccat taacgtctcg
     1741 taagggtatg cgctgtattc tgtcaagcac aaggaaatta gacaatatcg gcatatagaa
     1801 aattgaattt ttgttgacat cttgtgcgcc gcacaaaaag tggctcacca aattttttta
     1861 aaggagtaac ttatggaaca acaaattcaa cagattgtta attttgcaat tggcacagtg
     1921 cgtggtttag gcgatgcagc gaaaaatacg ctcaccagct tagaagcgga agtgaaatca
     1981 ctcgtcgata aaggcgcagc cgccagcgac gaaaatgcac aaaaaatacg tgccaatgca
     2041 accgaccttg ttaaccgtgt cactaacatt gttaacaccg ctgtcaacaa tctcaacgat
     2101 gttaccggtc aagcacagaa actcgtgcaa gacgcaatcg cgaaagttaa gcctgaatca
     2161 aaacaaggct aatcactatt acatattttg cgcgcaatgc gcgcaaaata tgtagttttt
     2221 atctttttat taacggcaca aatggacgtg ccttttcacc gttatagatt tggcgggggc
     2281 gaccaatctt tgctgcaggg ttttcaatca tctctttcca ttgcgcaatc cagccgggca
     2341 tacggcctaa agcaaacatt actgtaaaca tctcagtggg gatgccaatc gcgcggtaaa
     2401 tgagaccact gtaaaaatcg acgttaggat aaagtttacg ctcgacaaaa taatcgtctt
     2461 tcagcgcggc ttcttccagt tgcaaagcga tatctaaaag tggatcctta acccccagtt
     2521 tagcgagtac atccatacac gcttttttaa taatttttgc acgggggtcg aagtttttgt
     2581 atacgcgatg gccaaagccc atgaggcgaa aattggaatt tttgtccttc gcgagagtga
     2641 caaatttctt aacatcgcca ccttctttgt ggatttgctc gagcatttca atcaccgcct
     2701 gatttgcacc gccgtgaagc ggtccccaaa gcgcgttgac accgctggaa atagcggcaa
     2761 atacatttgc acgcgatgag ccgactaaac gcacggtcga cgtggagcag ttttgctcgt
     2821 gatcggcgtg taaaatgagg agcaaatcca aggcgcgttc cacaacagga tcggcggtgt
     2881 aattttctgc gggtaccgca aaaagcatac ggataaaatt ggcgatgtaa ctcaacgaat
     2941 ttttaggata catcatcggt tggccaatcg attttttata cgaaaacgcc acaatcgtcg
     3001 gcgtctttgc caaaaggcgg ttaatcgaaa gttcacgacc aattggatcg agaggatcgg
     3061 acgcgtcctt aatatagtag cccgagcggg tattaatcat tgagctaagt gtcgccattg
     3121 ggtgcgcgtc tttgggaaag ccgtcgtaaa gacgcttcaa gtcttcgtgg atgagagtgt
     3181 ggcgtgtgat agtctgcttg aagttttcaa atacctgcgc gttggggaga tttccataaa
     3241 tgagtaagta agccacttct aaaaaagttg aattttcggc gagctctgca atgtcataac
     3301 cgcgatagcg taaaattcct ttttcgccgt ccaaaaaagt aatcgaactc atcgtcgagc
     3361 cggtgttgac atagccatca tctaacgtga caagccctgt ttgcgcacgc aattttgcaa
     3421 tgtcgattgc gagttcattt tcagtgccgt ggattacggg cagttcgtat tcgttaccgt
     3481 cataaataag tttagctgta tgttccatac gtcaaaataa gaatgttatc ggggctttca
     3541 agaaatatcg tcaaatccat gaaatctata tataaagcct gtgcgcgcat aggctctaac
     3601 gtttgacgcg ctatctttat ctttatatct ttcgggacaa tgcctgattt aatcgccgaa
     3661 attaatgccc ttgaaaaaag agccgcagag caaatcgccg ccgctgccga tgccaatgca
     3721 ttgcaaaaaa ccacacagga atttatcggc aagaaaggtg tgcttaccgt agtgatgaaa
     3781 caacttggca cgctctcgaa cgaagagcgc ggcgcagtag ggaaggcggc aaatgcattc
     3841 aaagttaaac tcgaagagct cctcgccgcc aaagaggctt cactcgccga agcaaaatac
     3901 gcgtcgctcg ccgaaacaga atttatcgac ctctcattta ctccccctga atttgcggcg
     3961 gtgcaaacgg ggagtgcgta ttcggggcat gtccacccca tcgcccaaac tcaaaaaaaa
     4021 ctcgaagata tttttacgtc gatggggttt tccattgtcg atggtccaca aatcgaagat
     4081 gattatcaca attttggcgc gctcaacttt accgacgacc atcccgcgcg tgatatgcaa
     4141 gacacttttt atgtaacgca cggcggcgca cagcaggcaa ccaacaccac cgcttacctt
     4201 ttgcgcacgc acacgtcgag tgtgcaaatc cgcgcgctcg aaaaaatgca accgccgctg
     4261 cgcatcattg cacccggccg agtattccgc tacgaagagg tcgatgcctc gcacgaacac
     4321 acattttatc aaatggaagg catgattatt gaccgccata tttctgtcgg caatctgtta
     4381 cacctcatgc agacattatt aacggaagtt tttgggcgta aggtggaagt ccgcttgcgt
     4441 ccgggatatt ttccgtttgt ggaaccggga tttgagttgg atatgaagtg tttagtatgt
     4501 gaatccgcag actcgcgcga ggatgacatt cgtggcggcg caacccccgt gacatccgtg
     4561 tcaactggct cgcacaccga tggcctccat gccagtgcga gccccgtgac atccgtgtca
     4621 ctgggatgtt ccgtctgcaa atattctggt tgggtggaag tgttgccctg tggattggtg
     4681 catcccaacg tgttgcgcaa cattggcgtt aatccttccg catggcaggg ggctgcattt
     4741 ggtttagggc ttaaccgtct cgtcatgatg caacacggca tcgaagacat ccgccacttc
     4801 atgtcaggaa aacttagttt cttgcgacaa ttttaacggg gttgtatgac atttgaaaca
     4861 cgcgcattag gccgcattga aattgaagct tcgaaacaac ttttttttcc cgaggggctt
     4921 tttgcatttg aagcgtttcg ccacttcgca ctcttgccta caaaatcagg tagcacattc
     4981 cattggttgc aatcgcttga ggaaacgcgt ctcgcgtttc tcgtcacccc catcactgaa
     5041 atcacaaggc gctataagcc cagtattcaa gaacagtatc tcgccgtcat tgaagcgcaa
     5101 acaatagcgt cgttggaaat ttggggaatt attactgttc ctgctggtaa accggaaaat
     5161 atgacgatca acctacaagg accgattctc atcaaccgcg aaaagaaact cggcagccag
     5221 tttgtatcgg acaacaccga gcacttggtg agaacgccgc ttttagattt agtcgatgaa
     5281 gataaaaaaa acgctgaagg gaattcataa tgctggtgct cgcgcgccgt gtcaacgact
     5341 cgattgtcat tggcgacagt gtggaaattg ttgtcgttga aattaagggc gatcaagtta
     5401 aactgggcgt gaaagctccc aaggacgtta aagtattccg tggtgaaatc cacgctgaaa
     5461 ttcaaaaaga aaataaggcg gcgttagaat cgcaattacc gcccgacctt gatacacttt
     5521 tgagtaagaa aaaataatgc gcgttataga gcttttgcgg caaatatttt ttattaaact
     5581 ttatgttcga cgcaaagaat cctcaataca attaatttta acgggaattc tttcatgatt
     5641 tattctct
//
LOCUS       DAOMPD010000130         5637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6058_length_5637_cov_2.433178, whole genome shotgun sequence.
ACCESSION   DAOMPD010000130 DAOMPD010000000
VERSION     DAOMPD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6058_length_5637_cov_2.433178"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3045
                     /gene="gltB"
                     /locus_tag="PLY93_05810"
     CDS             <1..3045
                     /gene="gltB"
                     /locus_tag="PLY93_05810"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRP69030.1"
                     /translation="AALSELPQMLYRYFKQLFAQVTNPPVDAIREELVMELTTYVGPE
                     QNLLDETPHHARRIELPHPIIDNIQIEQFKEIAKRGFSAQVFPLHFNPSKKNDMRTQI
                     EKMCAAVCEAVKQGTNIVILSDRGVSESVAPIPSLLGVAAVHHALIRAGLRTRTGIVL
                     ESAEAREVHHFALLLGYGANAINPYLAYETLADLYSIGFLPEIKNEEYVEKNYRKAVA
                     KGLFKIFSKMGISTLQSYCGAQIFEAIGLDTEIIEHYFTGTPSRIEGVSLEMLEEETR
                     RRHRGAFDSMKEGNLESGGLYHYRVDGEAHLWNPTTIAKLQISTRTSDYKTFKEYTQA
                     VDNQTERHVTLRSLFELDYAANPIPIEEVEPAKEIVKRFATGAMSFGSISWEAHTNLA
                     IAMNRIGGKSNTGEGGEGSERFVTLSNGDSLRSAIKQVASGRFGVTANYLVNADELQI
                     KMAQGAKPGEGGQLPGFKVDKIIGKVRHSTPGVTLISPPPHHDIYSIEDLKQLIFDLK
                     NINPRARISVKLVSESGVGTVAAGVAKAHADHILISGHDGGTGASPISSIQYAGTPWE
                     LGLAEAHQVLVVNGLRDRVYLQTDGQLKTGRDVVIAALLGAEEFGFATAPLVTLGCIM
                     MRKCHLNTCPVGVATQDPELRKKFTGKPEYVVNFMFYIAEEMREYMAKLGFRKVTDMV
                     GRSEKLVVIRPKNHWKARGIDLSKLLVAQTPLYKTDLYRTKAQNHEIDKQIDNRIIEM
                     AKPALDNEEPVSIELPVTNVDRTVGAMLSGEIAKRYGEAGLKDDTITIKTHGYAGQSF
                     GCFLVTGVTLSLSGQANDYCGKGLSGGKLIVHTSEKADYDPAENIVIGNTCLYGATSG
                     EAYFAGRAGERFAVRNSGALAVVEGTGDHGCEYMTGGCVVVLGRIGRNFAAGMSGGIA
                     YIWDPNALNEKFVNRELVEFEYINDSKDTNELLSIVKKHYTLTHSKRAKKLLDDWSHQ
                     IKHFKKIIPTEYKKVIEARSAEKKIDTASQGKSAEKVEVHHG"
     gene            3038..4477
                     /locus_tag="PLY93_05815"
     CDS             3038..4477
                     /locus_tag="PLY93_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRP69031.1"
                     /translation="MGKITGFMEYDRASQKALAPKERVKNFKEFEETFSEETAKTQGA
                     RCMDCGIPFCHGDTGCPVDNLIPEFNDLVYRGHLEAAVENLLSTNNFPEFTGRLCPAP
                     CETACTLGINEPPVAIKAMERFIIDTGFKNGWVKPRPAKIKTGKRVAVVGSGPAGMAA
                     SQQLARMGHSVTLFEKNEKIGGIIRFGIPDFKFEKWQIDRRAEQLVAEGVEIKIGITV
                     GKDIAVEKLLADYDAIVLAGGAEAPRDLPIPGRELKGVYYAMEFLPQNNRMNAGISVK
                     DAISAKDKHVVVIGGGDTGSDCVGTSHRHHAASVTQLELFPMPPLERAPSTPWPYWPM
                     KLRTSSSHEEGGERMWSINTLRFNGDDKGNVKSLSCIKGEIKAGKFVESPGSEFELKA
                     DLVFLAMGFLGPVKQGMIQDFEKHGLTLDARGNVKADFGNHAGAHRTSVEKVFAAGDM
                     RRGQSLIVWAIAEGRKCANAVNTYLSQTR"
     gene            4519..>5637
                     /gene="glnA"
                     /locus_tag="PLY93_05820"
     CDS             4519..>5637
                     /gene="glnA"
                     /locus_tag="PLY93_05820"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRP69032.1"
                     /translation="MAKQAFNTWKEVIDFAKKNNVLFYDFRFSDIGGAWHHVSYHTDA
                     ISEDSFKGLPFDGSSIAKWQPIHRSDMQLIPDREGAFLDPFTADPTLVVFCDVYDIYK
                     NQMYEKCPRSIAKKALEHLKSTGIGDTAYFGPENEFFIFDSVRVRDDINCQYYEIDSN
                     EGSWNTATANYNDGHNLGHRPGTKGGYFPVSPRDSQVDIRAEIVKTLHQIGMETFVVH
                     HEVAQAQGEIGVKFGTIIEAADNVQKLKYVVKNVAKRHGKTATFMPKPLFGDNGNGMH
                     VHQSIWKNGENTFAGDKYQGLSDTALHYIGGVLKYARAIAAFSNASTNSYKRLIPGFE
                     APSILTYSAQNRSASCRIPFVSGAKAKRVEFRFPDSTAN"
BASE COUNT         1553 a         1358 c         1452 g         1274 t
ORIGIN      
        1 gccgcgctct ccgaattacc gcagatgctc tatcgatact ttaaacaact tttcgcgcag
       61 gtgacaaacc caccggtcga cgccatccgc gaagagttgg tgatggaact cacaacgtac
      121 gtggggccag agcaaaactt gctcgacgaa acaccacacc atgcgcggcg catcgagtta
      181 ccgcacccta tcatcgacaa cattcaaatc gaacagttta aggaaatcgc aaagagggga
      241 ttttccgcac aggtttttcc attgcacttt aatccgtcga aaaaaaacga tatgcgcacc
      301 cagatcgaaa aaatgtgtgc ggctgtttgc gaggctgtaa aacaaggcac gaacatcgtt
      361 attttaagcg acagaggtgt gagtgaaagt gtcgcgccaa tacccagctt gttgggtgtc
      421 gctgctgtgc accacgcact catccgtgct ggcctcagaa cccgcaccgg cattgtgctc
      481 gaatcggcag aggcacgtga agtgcaccac ttcgctttac tcttgggcta tggagccaat
      541 gccatcaacc cctatttagc gtacgaaacc ctcgccgatt tgtactcgat tggttttcta
      601 ccagaaatca aaaacgaaga gtacgttgaa aaaaattacc gcaaggcggt agcaaaaggg
      661 ctttttaaga ttttcagtaa aatgggtata tccacgttgc aatcgtattg tggcgcgcag
      721 atttttgaag ccattggttt ggatactgaa attatagaac actacttcac cggcacgccc
      781 agtcgcatcg aaggggtaag tctcgaaatg ctcgaagaag aaacccgccg ccgccatcgg
      841 ggagcgttcg actccatgaa agaaggcaat ttagaatcgg gtgggcttta ccactaccgc
      901 gtcgacggag aagcgcattt gtggaatccc acaacgattg ccaagttgca aataagtacc
      961 cgcacgtcgg attacaaaac tttcaaagag tacacgcagg cggtggataa tcaaaccgag
     1021 cggcacgtca cgttgcgttc tttatttgaa ttggactacg ctgcaaatcc cattccaata
     1081 gaagaagtgg aaccggccaa agaaattgta aagcgcttcg ccaccggcgc aatgagtttt
     1141 ggttctattt catgggaagc gcacaccaac ttagcgattg ctatgaaccg tattggcggt
     1201 aaatccaaca ccggtgaagg cggcgaaggt tctgagcggt ttgtcacact ctcgaacggc
     1261 gattctctac gctcggcgat taagcaagtg gcatcgggac gttttggcgt caccgcaaac
     1321 tatttagtca acgccgatga actccaaata aaaatggcac aaggcgcaaa acccggcgag
     1381 ggcggacagt tgcctggttt taaggtcgat aaaattatcg gcaaagtgcg tcactccact
     1441 cctggtgtga ctctcatctc accgccgccg caccacgata tttattcgat tgaagatttg
     1501 aaacagctca tcttcgattt aaaaaatatc aacccacgcg cacgcatttc ggtgaagctc
     1561 gtttccgaaa gcggtgtagg taccgtggcg gcaggcgttg cgaaagcgca tgccgaccat
     1621 atcctcatta gcggtcacga tggcggcacc ggtgcatcgc ccattagctc gatccagtac
     1681 gcgggcacac catgggaact cggtcttgcc gaagcacatc aagtgctcgt cgtcaatggt
     1741 ttacgcgatc gtgtgtactt acaaactgac gggcaactga aaacagggcg tgacgttgtc
     1801 atcgccgcgc tcttaggcgc tgaagaattt ggttttgcca ccgcgccgct ggtcacattg
     1861 ggttgcatta tgatgcgcaa atgccacttg aacacctgcc ctgtaggtgt ggcaacgcaa
     1921 gaccccgaac tacggaaaaa attcaccggt aagccagagt acgttgtgaa tttcatgttt
     1981 tatatcgccg aagaaatgcg cgaatatatg gcaaagttgg gatttcgtaa agtgaccgat
     2041 atggttggtc gctcggaaaa actggttgtc attcgcccca aaaaccactg gaaggcgcgt
     2101 gggattgacc tgtccaaact tttagtcgcg cagacaccgc tttacaaaac cgatttgtac
     2161 cgcaccaaag cgcaaaacca tgaaatcgat aagcagatcg acaaccgtat tatcgaaatg
     2221 gcaaagcctg ctttggataa cgaagaaccg gtatcgattg agttgcccgt caccaatgtt
     2281 gaccgcaccg tcggtgcaat gctctcgggt gagattgcta aacgctacgg cgaagcggga
     2341 ttaaaagacg acacaatcac cattaaaact cacggttatg ccggccaaag tttcggctgc
     2401 tttttggtaa cgggcgttac gctaagcctt agcggccaag cgaacgacta ctgcggcaaa
     2461 ggtctttcgg gaggaaaact cattgtgcac acctctgaaa aagccgatta cgatcctgcg
     2521 gaaaatattg tcatcggcaa tacctgcctt tacggagcga catcgggcga ggcgtacttt
     2581 gcaggccgtg ccggcgaaag attcgcggtg cgcaactcgg gagcgctggc tgtcgttgaa
     2641 ggtaccggcg accacggctg cgaatacatg acgggcggct gcgttgttgt tttggggcgc
     2701 attgggcgca actttgccgc aggtatgtcg ggtggtatcg cgtatatttg ggatcccaat
     2761 gcactcaacg aaaaatttgt gaaccgcgag ttggttgagt tcgagtatat aaacgactcc
     2821 aaagatacga atgaactttt gagtatcgtg aaaaaacact acacactaac gcactcgaag
     2881 cgcgctaaaa agcttttaga cgattggagt caccagatta aacacttcaa aaaaattatt
     2941 ccgacagaat acaagaaggt catcgaggca cgaagcgccg agaaaaaaat cgatacagcg
     3001 tcgcagggta aatccgccga aaaagttgag gtacaccatg ggtaaaatca ccggatttat
     3061 ggaatacgac cgcgcatcac aaaaagcact tgcgcctaaa gagcgcgtca aaaatttcaa
     3121 agaatttgaa gaaacctttt cggaagaaac ggcaaagacc caaggcgcac ggtgcatgga
     3181 ttgcggcatc ccgttttgcc acggtgacac cggctgcccc gtcgataact tgattcctga
     3241 gtttaatgat ttggtgtacc gaggccatct ggaagcggcg gttgaaaatt tactctcgac
     3301 aaataatttt cctgagttca caggaagact ttgccccgca ccgtgcgaaa ccgcgtgtac
     3361 gttgggcatc aacgagccac cggttgccat taaagcgatg gagcgtttta tcatcgacac
     3421 cggttttaag aacggttggg taaagccccg tcctgcaaaa ataaaaacag gaaaaagggt
     3481 ggcggttgtg ggttcaggcc ctgctgggat ggcggcgtcg caacaacttg cgcggatggg
     3541 gcactcagtg accctttttg agaaaaacga aaagattggc ggtatcattc ggtttggcat
     3601 tcccgatttt aagtttgaga aatggcaaat cgaccgccgc gcggaacaac tcgttgccga
     3661 aggtgtggaa attaaaattg gtataaccgt cggtaaagac attgccgtgg aaaaacttct
     3721 cgccgattac gatgcgattg ttttggcggg cggcgcagag gccccacgtg atttaccgat
     3781 accaggccgc gaacttaaag gagtttatta cgccatggaa tttttgccgc agaacaaccg
     3841 gatgaatgcg ggtatttcgg taaaagacgc catctcggct aaagacaaac acgtcgttgt
     3901 gattggcggc ggcgacaccg gctccgactg cgtgggtact tcgcaccgcc accacgcggc
     3961 gtcggtgacg cagttggaac tttttccgat gccgccctta gagagagcgc catcgacacc
     4021 gtggccgtat tggccgatga agttacgcac atcgtcaagc cacgaagaag gcggcgagag
     4081 gatgtggtcg attaacacat tacgcttcaa tggtgacgac aaaggaaatg taaagagcct
     4141 cagttgcatt aaaggcgaaa taaaagcagg taagtttgtt gaaagtcccg gttctgaatt
     4201 tgaactcaaa gccgatttag tctttttagc gatgggcttt ttaggccccg ttaaacaagg
     4261 tatgattcaa gactttgaaa agcacggctt aacgctcgac gcgcgtggta atgtcaaagc
     4321 cgactttggc aaccacgccg gtgcgcacag aaccagcgtc gaaaaagttt ttgctgcggg
     4381 tgacatgcgc cgtggccaaa gcttaattgt ttgggcgata gccgaagggc gcaaatgcgc
     4441 aaacgcggtg aatacttatt tatcgcaaac acggtagaaa atttattgag aagtcaagag
     4501 aaaataaagg agatttttat ggcaaagcaa gcgtttaaca catggaaaga ggtgatcgat
     4561 ttcgcaaaaa agaataacgt acttttttac gattttcgtt tttcagatat tggcggggcg
     4621 tggcaccatg tctcgtacca cacagatgcg attagcgaag actcgttcaa agggcttccc
     4681 ttcgacggca gctcgattgc aaaatggcaa ccgatacacc gttcggatat gcaactcata
     4741 cccgacagag aaggcgcgtt cttggatccg tttaccgcag atccaacact cgtcgtcttt
     4801 tgcgatgtgt acgatatcta caaaaaccag atgtacgaaa agtgcccacg ctcgattgca
     4861 aaaaaagcgc ttgagcattt gaagtcgacg gggattggcg acactgcgta ttttggtcct
     4921 gaaaatgaat tctttatttt cgactctgtg cgtgtgcgcg acgacatcaa ctgccaatac
     4981 tatgaaatcg actcgaacga aggttcgtgg aatacagcca cggctaacta caacgacggc
     5041 cacaacttag ggcatcgccc aggcacgaag ggcggttact tcccggtttc gccgcgtgac
     5101 tcacaagtgg acatccgcgc tgaaattgta aagacgctcc accagattgg catggaaact
     5161 ttcgtcgtgc atcacgaagt ggcacaagcg caaggggaga ttggcgttaa gtttggcact
     5221 attattgaag ccgccgacaa tgtgcaaaaa ttgaaatacg ttgtaaaaaa cgtcgccaaa
     5281 cgccacggca aaacggcgac gtttatgccc aaacctcttt tcggcgataa cggcaacggg
     5341 atgcacgttc accaatcgat ttggaaaaac ggcgaaaata ctttcgcggg cgataagtac
     5401 caaggtttga gcgacacagc tcttcactat attggaggag tgctcaagta cgcgcgtgcg
     5461 attgccgcct tttcaaacgc ctcgacaaat tcatataagc ggttaatccc ggggtttgaa
     5521 gcgccgtcga ttttaacgta ctctgcacaa aatcgttcgg catcgtgccg cattccgttt
     5581 gtgtcgggcg caaaagcaaa acgtgtcgag ttccgttttc ccgattcaac ggcaaat
//
LOCUS       DAOMPD010000131         5582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6134_length_5582_cov_2.519450, whole genome shotgun sequence.
ACCESSION   DAOMPD010000131 DAOMPD010000000
VERSION     DAOMPD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6134_length_5582_cov_2.519450"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2161
                     /locus_tag="PLY93_05825"
     CDS             179..2161
                     /locus_tag="PLY93_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP69033.1"
                     /translation="MPSKPKASSVYLRILAYAKPYRKRFILGIFMSIMLSIFNGLSLT
                     SLKPIFDVIETGASKPFQLHFEEKEINALVAAQKSHDLETLFTKHPAPEYYTAEKSWF
                     KEANRLKVNDTSNSITNFIAEKKLSMNLALVGLPAPAILKYVGIAVIPIFLLRLIFDF
                     GTVFFLTATGLLSVMDIRRALYTSMMRLPLSYFVREKTGVLMSRIINDVTTLSEATSI
                     ELRLSINNFFIIVTHIIILLIINAKLLMITAIGVPLILLPVSYFSRKIRYITRTEQTG
                     LADLNGHLQEVISGIRVIRAFTMESYQTKGFEVINHNLYLQNFRNRVSHQLGPALVEL
                     ATSVIVVGLIVYGGNLIIGGEMTSGSFFTFLFTLMILLRPIKQMATWFNLVQRAVASG
                     ERIFEIIDMKSEIEDPQNPKELPLLKKEIEFSHVGFRYPGTEKQVLRNIHFKAPIGST
                     VALVGHSGAGKSTLADLTLRFYDATEGSILFDGIDIRDVKIETLRNKVGMVTQDIFLF
                     NGTVKENISYGRTDISDKDIAEAAKMAYADDFIRLLPQGYDTIVGERGLMLSGGQRQR
                     LSIARALLKNPEVLILDEATSALDTQSERLVQRALEKLMKNRTTFVIAHRLSTIYNAD
                     LILVLKDGRIVERGTHQALLKKKGYYKKLFDMQFKE"
     gene            2210..2473
                     /locus_tag="PLY93_05830"
                     /pseudo
     CDS             2210..2473
                     /locus_tag="PLY93_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            2555..3112
                     /locus_tag="PLY93_05835"
     CDS             2555..3112
                     /locus_tag="PLY93_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP69034.1"
                     /translation="MAPEKITPNTDNKSEQKKPSPIKQIFLVLLLLIFLAALFYFFTQ
                     RFQCNKAPTPTTTQTVPVVNPPHVPPITAELKNFIESTKLNFVAEKTTLTPQTEKTLD
                     ALAGRLNGQNLRLQITGHTLGPRALNQKISEERAAIVKSALVARGVDAKNIETKGVAD
                     SESIADNNTPAGRAQNRRVSFKGLE"
     gene            3128..4732
                     /locus_tag="PLY93_05840"
     CDS             3128..4732
                     /locus_tag="PLY93_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRP69035.1"
                     /translation="MKKLNRFVGFLTFGMFVSVSFLSAAPATKTSTPVAMEKQSLPIV
                     DPSHAVIPPTPVDAQKELGKTLSIFTVIPFVIILLSIAILPLVIPHLWESNLIKAVIS
                     LFLAFIMAGYLATQGHLGFEVIVETLKEYYAFICLLIALFTISGGIYIEGDLKATPMT
                     NTTILGIGTLIASVIGTTGASVLLIRPLIRTNKERKYTTHIFVFFILLVSNVGGVLLP
                     VGDPPLFMGYLYGVPFFWTLIKLWPIYLTTASILLLVFFLWDMFAYSRENKTDLQKDL
                     SQQSKLRIRGGINFLLIAGVLLAVVFLKDYKTAHGQLELAWLQQPIMLGLALLSFVID
                     HIAKVKKTREHEEALLSKIQKEGKATPNMRVLTHYQAHTPRDKNNFSFAPIIEVAALF
                     IGIFITMIPALCILKARGAETGISQPWQFYWITGALSSFLDNAPSYATFFALGQGVTA
                     GMGSAAGAVVHATSGAINETILIGISVGAVFFGANTYIGNAPNFMVKAMCEESKVKMP
                     SFFGYMLYTILILVPVLGLVHLIFLR"
     gene            4738..5496
                     /locus_tag="PLY93_05845"
     CDS             4738..5496
                     /locus_tag="PLY93_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRP69036.1"
                     /translation="MSAQIHLFGLSYSPWSTQARWALDHHIIRYRYTEHVPLITNVQL
                     RLAAKNFTGKLTVPLLVTDTRVFDDSWHIVNYADRTGHREKLIPLDKADQIRAWHDLS
                     DAANKANRIIATDATSHNEAAKRAALPDFIPEFAKDTLTPLADMAAAYLSIKYDFRHA
                     IVAEEKRKVREALTKVRQALKGKNFIYDTFTYADITASGPLAFVSPVADKYIPLKPAL
                     REAFTQKDLATEFADLVEWRDKLYADKRGMPHSR"
BASE COUNT         1508 a         1412 c         1203 g         1459 t
ORIGIN      
        1 caccatcaac gacggtatgg ggaacacaat taccggcaca cgcggcatca ccaccgatcg
       61 atcgttgagc cgcactgtat ttaaaggcgg cataggttct gggcgcgcta atttttaacg
      121 aagagacgac aggctcctcg tttacgccgc gtcttatttc agatacttgg taaattctat
      181 gccaagcaaa cccaaagcaa gcagcgtgta tctgcgtatc cttgcctacg caaaacctta
      241 ccgcaagcgc tttattttgg ggatttttat gtctattatg ctttcgatct ttaacgggct
      301 tagcctcact tcactcaaac ccatttttga cgtcattgaa acaggggcgt ccaagccatt
      361 ccagttacat tttgaagaaa aagaaattaa tgccttggtg gcggcacaaa aatcgcatga
      421 tctggaaact ctcttcacca agcatcccgc tcccgaatac tataccgctg aaaaaagctg
      481 gtttaaagaa gcaaaccggc tcaaagtgaa cgacacttca aattcaatca ccaatttcat
      541 tgccgaaaaa aagctttcga tgaatcttgc tcttgtggga ttacccgcgc cagcaatctt
      601 aaaatacgta ggtatcgcag tcataccaat ttttttatta cgcttaattt ttgatttcgg
      661 tacagtcttt tttctaaccg caaccggctt actttcggtg atggatatcc gccgcgcgct
      721 ttacacgagt atgatgcgct tgccactttc ctattttgtg cgcgaaaaaa ccggtgtgct
      781 tatgagccgg attatcaacg atgtgacgac gctttcagaa gcgacttcga ttgaactacg
      841 cctttccatc aataattttt ttattattgt tacgcatata ataatactgc ttatcatcaa
      901 tgcaaaactg ctgatgatca ccgcgattgg agtgccactc attcttttac cagtgagtta
      961 tttttcgcgt aaaattcgct acatcacccg caccgaacaa accggtttag ccgatctcaa
     1021 cggccatcta caggaagtaa tttcgggcat ccgcgttatc cgcgcattca cgatggaaag
     1081 ctaccaaaca aaaggtttcg aggtgattaa ccacaatctt tatttacaaa attttaggaa
     1141 ccgcgtttca caccaactgg ggcctgcgtt ggtggagctg gccacctcgg ttatcgtcgt
     1201 cggccttatc gtctacggcg gcaatctcat cattggcgga gagatgacct cgggtagttt
     1261 cttcaccttt ctgtttacgc tcatgattct tttaaggccc atcaaacaaa tggcgacgtg
     1321 gttcaatctc gtgcaacgcg cagtggcgtc gggcgagcgt atttttgaaa ttatcgatat
     1381 gaaaagcgaa attgaagatc cacaaaatcc caaagagtta ccgcttctca aaaaagaaat
     1441 tgaattttct catgtgggct ttcgttaccc agggacagaa aaacaagttt tacgcaacat
     1501 tcatttcaaa gcgccgattg gttctactgt ggcgttggtg gggcattcgg gcgcaggcaa
     1561 gtcaacactc gccgatctaa ctctccgctt ttacgacgcc accgagggaa gtattctttt
     1621 cgacggcatc gatatccgcg atgtgaaaat cgagacgttg cgcaataaag tcggtatggt
     1681 cacccaagat atttttcttt ttaacggtac cgttaaagaa aacatttcgt atggccgcac
     1741 cgacatttct gataaagaca tcgccgaagc ggccaagatg gcgtacgccg acgacttcat
     1801 tagacttttg ccgcaaggct atgacaccat cgtgggtgag cgtggcctga tgctttcggg
     1861 ggggcagcgc caacgccttt cgattgcccg cgcactcctt aaaaatcccg aagtgttgat
     1921 tttagacgaa gccaccagcg cgctcgatac acaatccgaa aggctggtgc agcgtgcttt
     1981 agaaaagctt atgaagaacc gcaccacgtt tgtaattgcc caccgccttt cgacaatcta
     2041 caacgccgat ttaattttag tcttaaaaga cggaaggatt gtcgaacgcg gtacacacca
     2101 agcgttgctc aagaagaaag gatactataa aaagctcttt gatatgcagt tcaaagaata
     2161 aaatttccat ttgacatccg ccataaaaaa gaaggtaagt caaaagagca tggcgcaaaa
     2221 ctattacatt gtcattaaag gcaaaaagta cgaccgacga ttggtcgaac tcgccgaggc
     2281 gttgacctcg ggcaaaggcg atggccgcat ctccatccat gacgctaaaa cgctcttgag
     2341 cgcagtaaaa gactcgaata gttattcgcc gattgaaaaa ttgacaatgc aatatatccg
     2401 gtcgcgctat aaattcacca aagaaggcga tacgtatttt cgtggtgaaa tccgtaaatg
     2461 ggcggccaca aagaaaagca aaacgggcag caaggcatcg cgcactatca ccaaaaaagc
     2521 ttctacaact cccgcttctg cggcgcctgt ttcaatggcg ccggaaaaaa ttacacccaa
     2581 caccgacaat aaaagcgagc agaaaaaacc ttcgccgatt aaacaaattt tcttagtgct
     2641 tttattgcta atcttccttg ccgcactttt ctattttttc acccaacgct ttcagtgcaa
     2701 taaagcgccc acccccacta ccacacaaac tgttcccgta gtaaatcccc cccacgtacc
     2761 tccaattacg gcagagttga aaaattttat tgagtcgaca aagctcaatt ttgttgccga
     2821 aaaaacgacc cttacaccac agacagaaaa gacactcgac gcgttggcag gacgcctcaa
     2881 cggccaaaac ttacggttac agattaccgg ccacacgctc ggaccacgcg cgctcaacca
     2941 aaagatttca gaggagcgtg cagcgattgt caagagtgca ctcgtcgcac gcggcgtcga
     3001 tgcgaagaat atcgagacca aaggcgttgc cgacagcgaa tcgattgccg acaacaacac
     3061 acccgccgga cgcgcacaga accgccgtgt ttcattcaaa ggactcgaat aaaaaaggaa
     3121 agcatcaatg aaaaaactaa atcgattcgt gggatttctt acctttggta tgttcgtgtc
     3181 ggtaagcttt ctctccgctg cgcccgctac taaaacatcc acccctgtag ctatggaaaa
     3241 acagagctta ccgatcgtcg acccctcgca cgccgtcatc ccccctactc cggtggatgc
     3301 tcaaaaagaa ttgggtaaaa ccctctctat tttcacggta atccctttcg tcattattct
     3361 tctttctatc gcgatacttc cgctcgtgat tccacacctc tgggaatcga atttaatcaa
     3421 agcggtgatt agcctttttc tagcgtttat tatggctggc tatttggcta cacaagggca
     3481 tttaggtttt gaggtcatcg ttgaaactct caaagaatac tatgcgttta tttgtctctt
     3541 gatcgcactc tttaccattt cgggtgggat ttacatcgag ggcgatctta aagcaactcc
     3601 aatgacaaac accaccattc tggggattgg tactctcatc gccagcgtca tcggcaccac
     3661 tggtgcgtcg gtacttttaa tccgtccgct gattcgcaca aataaagaac gtaaatacac
     3721 gacgcatatt tttgtcttct ttatactact cgtctcaaac gtcggtggtg ttttgttacc
     3781 ggtgggcgat ccgccgcttt tcatggggta tctatacgga gtgccttttt tttggactct
     3841 cattaaactg tggccgattt atttaaccac cgcatccatt ttactcctcg tctttttctt
     3901 gtgggatatg tttgcgtact cgcgtgaaaa caaaactgat ttacaaaaag atttatcgca
     3961 acagtcaaag ttacgtatcc gtggcggtat caactttctc ctcattgcag gagtactctt
     4021 agcggttgtc tttttgaaag actataaaac agcgcatggg cagttagagt tggcgtggct
     4081 gcaacagccg ataatgcttg ggcttgcgct gctttcgttt gtcatcgacc acatcgccaa
     4141 agtcaaaaaa acacgcgagc acgaagaagc gcttttatct aaaattcaaa aggaaggcaa
     4201 agcgacgccc aacatgcggg tgttgacgca ctaccaagca catacaccgc gcgataaaaa
     4261 taattttagt tttgccccca tcatcgaagt agctgcgctc tttattggca tttttatcac
     4321 aatgatcccc gcgctttgta tccttaaagc gcgcggcgca gaaacgggga tcagtcagcc
     4381 gtggcaattt tattggatca ccggtgcatt gagttcgttc ttagataacg ctccttcgta
     4441 cgccacattc tttgcgttag gccagggcgt aacggcgggc atgggctcgg ctgccggtgc
     4501 ggttgtccat gcgacatcgg gagctatcaa cgaaaccatt ctcattggta tttcagtagg
     4561 tgcagttttc ttcggcgcca acacgtatat tggtaatgcg cccaacttta tggttaaagc
     4621 gatgtgcgaa gagtcgaaag taaaaatgcc gtcgtttttc ggttatatgc tttacacaat
     4681 actgattctt gtacccgttc tgggattagt ccatcttatt ttcttaagat aaattctatg
     4741 agcgctcaaa ttcatctctt tggtttaagt tattcccctt ggtcgacaca ggcgcgttgg
     4801 gcactcgatc atcatattat ccgctaccgc tataccgaac acgttcctct tatcaccaac
     4861 gtacaactac gattggctgc aaaaaatttc acaggcaagt tgaccgtccc cctacttgtc
     4921 accgacacgc gcgtcttcga cgactcgtgg cacatcgtca actacgccga tcgcaccggc
     4981 caccgcgaga agttaatccc tctcgacaag gcagatcaaa tacgtgcatg gcatgattta
     5041 agcgatgcag cgaataaagc caaccgtatt atcgccaccg acgccacaag ccacaacgaa
     5101 gcggcgaaac gggcggcgtt acccgatttt atacccgagt ttgccaaaga tacgctcact
     5161 ccgcttgccg acatggcggc ggcgtacctt tctatcaagt acgattttcg ccatgcgatt
     5221 gttgccgaag aaaaaaggaa ggtacgcgaa gcgctcacta aagtacggca agcgctcaaa
     5281 ggtaagaatt ttatctatga cactttcact tatgccgaca tcacagcgtc cggcccgctt
     5341 gcgtttgtga gccccgttgc cgataaatat ataccgctta aaccggcgtt acgcgaagcg
     5401 tttactcaaa aagacttggc caccgagttt gcggatttag tcgaatggcg cgataaatta
     5461 tatgcagata aaagaggtat gccgcactcg cgctaatgca aaatttgcct accgccgctt
     5521 gcgcttcgtc ttagttcagg ggcaaacaag cggtggaaga tttttccacg ccttcttgaa
     5581 tc
//
LOCUS       DAOMPD010000132         5577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6144_length_5577_cov_2.558131, whole genome shotgun sequence.
ACCESSION   DAOMPD010000132 DAOMPD010000000
VERSION     DAOMPD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6144_length_5577_cov_2.558131"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLY93_05850"
     CDS             complement(<1..968)
                     /locus_tag="PLY93_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP69037.1"
                     /translation="MKRNRWVILLLFMLPAAVSQLLWLNFAPLLTLVQKKYAVDELTA
                     NLLILVFPLVYVLVSIPAGYLTDKRGYKYAISLGSIITAIFALLRIYDTHFVFLLACQ
                     VGIAVGQPFIINGISKLVADWFSPKQNALATGIGTLGMFIGMALGMGLAVPLDTALGF
                     RGAMAAFAAIAVLSALLFVLFVRENNTAPSESTAQMGRELKSLLQSKNLVLLFIIAFM
                     ALGYFNGLTSWLEQILAPNGVSSAAAGNAGAALIGGGILGAALIPALSDLVRRRKPFL
                     IGCCFIAGALTWPFCKSGENYTLYLLSALMGFFFLPGYALLLSATE"
     gene            1075..1338
                     /gene="fliQ"
                     /locus_tag="PLY93_05855"
     CDS             1075..1338
                     /gene="fliQ"
                     /locus_tag="PLY93_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRP69038.1"
                     /translation="MNEADVISVMRDAVWLTIKLSAPALLTSLLVGLVISIIQTTTSI
                     QEQTLTFVPKLIAVLIVLLFFASWMIHSITDYTRDLMHLIGRQ"
     gene            1370..2158
                     /gene="fliR"
                     /locus_tag="PLY93_05860"
     CDS             1370..2158
                     /gene="fliR"
                     /locus_tag="PLY93_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRP69039.1"
                     /translation="MEIIGYQFQLFSLIFARFLALFSVAPGFSGDSLPFLSRFALAFL
                     AAAIITPVAQMPSGFEEVVHNSYIMIVFEQAALGIFIGVVMQFLFAAYQLAGEFFSVQ
                     MGFGISEVLDPLSQTSLPIIGTLKNLIALFVFFVSGAYAYLIQALAFSVERVPHLGYS
                     FLKGGVTQNHLFKYFALLSGGMFISALKIALPVMGALLLVSVTLGVLSKAAPQMNMLV
                     LGFSVKIVIGFLALMALSPLIVQTMFAEMDRSLSILDSLIKAWR"
     gene            complement(2199..3026)
                     /locus_tag="PLY93_05865"
     CDS             complement(2199..3026)
                     /locus_tag="PLY93_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69040.1"
                     /translation="MIAFIYMPLSAEEVNTNKPSAPSTESKVETPSYENNKTESPSPA
                     KSKAAPETAVKPDTEADQTETPKSDTDDKDTHKKKKHVIKLIDDKEATPLPERDPATF
                     ELSNRRMLDDYTELERRPYDRGEHILIAALMGAVGGGFVGGLVGFSGYDKDDETKSKN
                     ALYTFGGIGAGVGVLTGVTTTFFERGKIEQFVIGKFLLKYSWYGALGGALIGGGIGFI
                     PYASSGNYSDILRYGGYGAGVGLLAGLTFFFIDLPGQLQLYTFQRGDQSGLVLALHF"
     gene            complement(3085..3852)
                     /locus_tag="PLY93_05870"
     CDS             complement(3085..3852)
                     /locus_tag="PLY93_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRP69041.1"
                     /translation="MWRSFIPNAFSMGNLTFGFFSIVIASKANGHNSENFYFFIASGL
                     CIMIAAIFDGLDGPLARLFKSQSQLGEQLDSLADLTTFGIAPGFLMYKIYLFDLVISK
                     TTLGTPWDIPLGMAAAAVFPLCAAFRLARFNITHDPSSFVGLPSPVAGLMIAFIPMYN
                     NNSEYISRPIAVSIFLLVAFLMISNIRYSKPQARIKPHFTILRLIGFIAIISLLIYRF
                     NLYYVVFGVVILYIASGLFAMSIHLIQKLHYKITSGD"
     gene            3970..>5577
                     /locus_tag="PLY93_05875"
     CDS             3970..>5577
                     /locus_tag="PLY93_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRP69042.1"
                     /translation="MLLEELGKLEKEQLLKVAEVWAIPKPPGEKKALIQLLYKTFIDP
                     YHLKTVLEKLTPLQVKIYAVILNNKQILTLGEVSRKIQLQPINVEKELAVLKHIMLLY
                     QKKNRERITHNLDKYIPFDEFRKLVSTDGNPRGEKFQISIRREVEQTSPEEHDSKYLT
                     ILGGKGKKPREYAEKAYKDETLAKTLATLSQGEMVLIDEAFTNGGIIEINAARIIMDE
                     QKLPMEKTIRRLDAFQLLKDIYFIDERFVRILVIPLELFNYLKKTPLFPIRNDVKELT
                     ERTIENGFDFLLNLKKLLLFISNKGLTLSQSEKLRQADMKRSEASLIDIDINLFSEKS
                     QMHQIEIILPLLRLFELVDLRDENVVLIENYEDFLKREPAELMRDLFNKILEAADKRM
                     VGDEVFLPVDLPFFKMPMIDTCVKLIENNEGMFVKVLLAELIRSRVIMTPGFKVRDFK
                     NAYLEQRSMIVSAMIYMYLMGMLQVDYPKRFVNISQLGNHFFHEAPLTTESSSGAIIL
                     NPDGTLVAIPEKMSLHDLHLLKSFAELK"
BASE COUNT         1600 a         1286 c         1228 g         1463 t
ORIGIN      
        1 tcttcggtgg ctgacagtaa aagcgcataa ccaggcaaaa agaaaaaacc catgagcgcg
       61 ctcaaaagat agagagtata attttctcct gatttacaaa aaggccaagt gagcgcgcct
      121 gcgataaaac agcatccaat caaaaacggt ttgcgccgac gcactaaatc ggacaaagcg
      181 ggaataagcg cagcgcctaa aataccgccg ccgatgagag cggcgccggc attccccgct
      241 gctgcgctcg atacgccgtt gggggcaagg atttgttcca accaactggt gaggccattg
      301 aaatacccca acgccatgaa agcaataata aaaagaagta ctaaattttt cgattgaaga
      361 aggctcttca gttcgcgccc catttgagcg gtggattcac tcggtgctgt gttattttcg
      421 cgaacaaaca acacaaaaag aagcgcactg agaacggcga tcgctgcaaa cgccgccatc
      481 gctcccctaa atcccaacgc agtgtccaac ggcacggcaa gccccatacc gagtgccatg
      541 ccaataaaca taccgagcgt acctatgccc gtggcgaggg cattttgttt gggggagaac
      601 caatcggcga cgagtttaga aataccgttg atgataaaag gttgccctac tgcaatcccc
      661 acttggcaag cgagtaaaaa aacaaagtgt gtatcgtaaa tccgcagcag cgcaaaaatg
      721 gcagtgatga ttgaccccag tgaaatggcg tatttgtagc cgcgcttgtc ggttaaatag
      781 ccagcgggta tcgaaaccaa cacgtagaca agtggaaaaa ctaatataag caaattggca
      841 gtcagttcat cgaccgcgta ttttttttga acgagagtta aaagcggcgc aaagttcaac
      901 cacaagagct gactcaccgc cgctggcagc ataaaaagaa gtaggatgac ccagcggttt
      961 cgtttcatag aacacaaatt tgaaaaggcg tgaggggtac aatccataag caatttctat
     1021 tattataaac atataaaaaa ttgacggcgc atctgtatta tgtcacattt acacatgaat
     1081 gaagccgatg tcattagtgt catgcgcgac gccgtttggc tcacgattaa attgagtgca
     1141 ccggcgcttt taacttcgct tttggtggga cttgtcatca gtatcattca aacaactacc
     1201 agcattcaag aacaaacgct cacgtttgtg cctaagctca ttgcagtgct catcgtgctt
     1261 ctttttttcg cctcgtggat gatccacagc atcactgatt atacccgtga tttaatgcat
     1321 ctgataggta ggcaataaag tacagcgcgt ctaagcatcc ccgcagcccg tggaaattat
     1381 cggttaccag tttcaactct tctcgcttat cttcgcgcgc tttttagcgc tcttttcggt
     1441 agcacctggt ttttcgggag actcccttcc gtttctctcg cgctttgcgc tcgctttttt
     1501 agcggcggcg atcatcaccc ccgtagcaca aatgcccagc ggttttgagg aagtcgtcca
     1561 caattcgtat attatgatcg tcttcgaaca ggcggcactc ggtattttta ttggcgtcgt
     1621 gatgcagttt ttgttcgccg cgtatcaact cgccggtgaa tttttttcgg tacaaatggg
     1681 gttcggtatc agtgaagtgt tagatcccct ttcgcaaact tctctcccta tcattggcac
     1741 tctcaaaaat ttgattgcgc tttttgtttt ttttgtaagc ggcgcctacg cttacctcat
     1801 tcaagcgctt gccttttcgg tggaacgggt gcctcatttg gggtattcgt ttctcaaagg
     1861 cggggtgact caaaatcacc tattcaaata ctttgcattg ctttcggggg gaatgttcat
     1921 ttcggcgctc aaaattgcac tgccggttat gggtgcccta cttttagtct cggtgacgtt
     1981 gggtgttctt tccaaagcgg cgccgcaaat gaatatgctg gtgttgggat tttccgttaa
     2041 aattgtgatt ggttttttgg cgctgatggc gctctcgcct ctgattgttc aaacaatgtt
     2101 cgctgaaatg gatagatcgc tttcgatttt agattcgctc attaaagcat ggcgttaaac
     2161 cccgcacgaa ttacttggag tctggttgag aatcccagtt aaaagtgtag cgccaacaca
     2221 agaccgcttt ggtcgccgcg ttggaatgta taaagctgca actggccagg caaatcaata
     2281 aagaagaaag tcaatcccgc tagaaggccc acgcctgcgc cataaccgcc gtagcgtaaa
     2341 atgtcgctat agttgccgct cgatgcgtaa gggataaaac caataccgcc accgatgaga
     2401 gcgccgccta acgcgccgta ccatgagtat ttcaacaaaa acttaccaat cacaaattgc
     2461 tcgattttac cgcgctcaaa aaatgtagtc gtgacgcctg taagcacacc gacacccgcg
     2521 ccgatcccgc cgaacgtata tagtgcattt tttgatttag tttcgtcgtc tttatcgtaa
     2581 cctgaaaagc cgacaaggcc gccgacaaag ccgccaccca ctgcccccat gagcgctgca
     2641 ataaggatat gttcgccacg gtcgtaaggg cgtcgttcca attctgtata atcgtcgagc
     2701 atacggcggt tgctgagttc aaacgtggcg gggtctcttt cgggtaatgg agtcgcttct
     2761 ttgtcgtcaa ttaatttaat aacgtgtttc ttttttttat gtgtatcttt gtcgtccgta
     2821 tcgcttttgg gagtctctgt ttggtcagct tcggtgtctg gttttaccgc tgtttccggt
     2881 gcagctttgc tttttgccgg tgatggagat tctgttttat tattttcgta agaaggagtt
     2941 tctaccttac tttctgtact gggggcgctg ggtttgttgg tatttacttc ttctgccgac
     3001 aacggcatat aaatgaatgc aatcaccaaa aataagagaa tggtgcgcgt aaaaaaaaac
     3061 gtttgtatca atttagaaaa catgttaatc cccagaggta attttatagt gcaatttttg
     3121 tattaaatgg atggacattg caaaaagccc cgacgctata tataatataa cgacgccaaa
     3181 tacaacatag taaagattaa aacgataaat caaaagcgat atgatggcaa taaaaccgat
     3241 gaggcgtaaa attgtaaaat ggggcttgat gcgcgcttgt ggtttggagt agcggatatt
     3301 actaatcatc aaaaacgcca ccaacaaaaa aatgcttacg gcgatggggc gtgaaatata
     3361 ttcgctgttg ttgttataca ttggtataaa tgcaatcatg agtcccgcta ccggtgaagg
     3421 taaaccgaca aacgacgatg gatcgtgagt aatgttaaag cgcgcgagac gaaacgcagc
     3481 gcatagcgga aacactgccg ctgctgccat accgaggggg atatcccacg gtgtgcctaa
     3541 tgtcgtttta gaaataacca aatcaaagag ataaatttta tacattaaaa agccgggagc
     3601 aatcccgaaa gtggttaagt ctgccaatga atcgagttgt tcccccaact gactttgcga
     3661 cttaaaaagt cgcgctaaag ggccatctaa cccatcaaaa atagcggcga tcataataca
     3721 aaggccggaa gcgataaaaa aataaaaatt ttcggaatta tgcccatttg ctttagaagc
     3781 aatcacaatt gaaaaaaagc caaaagtaag gttacccatc gaaaatgcgt tgggtataaa
     3841 agaacgccac atgtttagat tacaatcgta actttgcttt gccccgacaa ggacattttt
     3901 cagcaagtac cgtttaaaat ttcataaacg attttacaga ctgtctctta tctgatgcta
     3961 tacccgcctg tgctactcga agagcttggt aaacttgaaa aagaacagct tctcaaagtt
     4021 gctgaagttt gggcaatacc taaacccccc ggtgagaaaa aagcgcttat ccaattactg
     4081 tataagacat ttatcgaccc ctatcactta aagactgttt tggagaaact taccccgcta
     4141 caggtaaaaa tctacgcagt tatcttaaat aacaagcaaa ttttaacgtt gggtgaagtt
     4201 agccgaaaaa ttcagctaca acccatcaac gttgaaaaag agctggcagt gctaaaacac
     4261 atcatgctgc tttaccaaaa gaagaaccgc gaacgcatca ctcacaattt agataaatac
     4321 atccctttcg atgagtttcg taaactcgtt tccaccgacg gaaatccacg cggcgagaaa
     4381 ttccagatta gcatccgccg cgaggttgaa caaacaagcc ccgaagaaca cgattctaaa
     4441 tacctgacaa ttttgggcgg caaaggtaaa aaaccacgcg aatacgccga gaaagcatat
     4501 aaagatgaaa ccctggctaa aactctggcg acgctgagcc aaggcgaaat ggttctcatc
     4561 gacgaggcat tcaccaatgg tggcattatc gaaatcaacg cggcacgcat tatcatggac
     4621 gaacaaaagc tgccgatgga aaaaaccatc cgccgtctcg acgcgtttca actgctcaaa
     4681 gatatttatt ttatcgacga acgttttgtg cgtattttgg ttatccccct tgagctcttc
     4741 aattacttga aaaaaacacc tctttttcca attcgtaacg acgtaaaaga gctcaccgaa
     4801 cggacgattg aaaacggttt tgatttctta ctcaatttga aaaagctact gctctttatc
     4861 tcgaataaag gtcttacgct ttcgcagtcg gaaaagctac gccaagccga catgaaacgc
     4921 tccgaggcat cgctcatcga catcgacatt aacctctttt cagaaaagag ccaaatgcac
     4981 caaattgaaa tcattctgcc gcttttacgc ctctttgaac ttgtcgactt gcgcgacgag
     5041 aacgtggtgc ttattgaaaa ttacgaagat ttcttgaaac gcgaacccgc cgagctcatg
     5101 cgcgatcttt ttaataaaat tttagaggcc gccgataaac gcatggtagg tgatgaagtt
     5161 ttcttacctg ttgatctccc attcttcaaa atgccaatga tcgacacttg cgtgaagctc
     5221 atcgaaaata acgaaggcat gtttgttaaa gtgcttttag ccgaactcat tcgttcgcgc
     5281 gtcattatga cgccgggctt taaggtacgc gatttcaaga acgcctattt agagcaacgt
     5341 tcgatgattg tgtcggcgat gatctacatg tatctcatgg ggatgttgca agtcgattac
     5401 cccaagcgct ttgtaaacat tagccaatta ggaaatcatt tctttcacga ggcgccactc
     5461 acgaccgaaa gcagctcggg ggcaattatc ttaaaccccg atggtaccct cgtcgcgata
     5521 ccggaaaaaa tgtcgctgca cgatttacac cttttaaaat cgtttgcaga actcaaa
//
LOCUS       DAOMPD010000133         5576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6145_length_5576_cov_3.360985, whole genome shotgun sequence.
ACCESSION   DAOMPD010000133 DAOMPD010000000
VERSION     DAOMPD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6145_length_5576_cov_3.360985"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLY93_05880"
     CDS             <1..334
                     /locus_tag="PLY93_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69043.1"
                     /translation="GAIVLTAGGVNDYFVAVPAGSSFNPLTTLPLTATPATTGVIKNL
                     MPGSYDVYRTCDKTKCANTSTAGTCDATTLGQDGFPEKAVPCGAAVTVTKGTTTSFGA
                     CAAAAGCP"
     gene            404..844
                     /gene="rplM"
                     /locus_tag="PLY93_05885"
     CDS             404..844
                     /gene="rplM"
                     /locus_tag="PLY93_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRP69044.1"
                     /translation="MDPLKGQRSHFPKRAELTHNWVVVDAKDKILGRVSTQIASLLMG
                     KNKVNYQPGQLVGDHVVVINASQVKLSGKKEETKEYIWHSRYYGGMKTRPYKTQMEKE
                     PEKAVLLTVKGMLPKTKYGRKLLTRVRVFTGAEHSLQGQKPAAV"
     gene            1003..1368
                     /gene="rpsI"
                     /locus_tag="PLY93_05890"
                     /pseudo
     CDS             1003..1368
                     /gene="rpsI"
                     /locus_tag="PLY93_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(1419..2720)
                     /locus_tag="PLY93_05895"
     CDS             complement(1419..2720)
                     /locus_tag="PLY93_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69045.1"
                     /translation="MFPFKKRILASKKIPLVLFNFVLLFLAVGCSNVVYRYQGGELTR
                     GTFYFMRDVYYQNQFEEEENPQARNRAILNDLADLDGIGALATKKGLKEDPSYDEYMF
                     RQEHARFGKAAIIHWQKKYKEKFNWQMLFQAEGLKYNEAAFMKEFASLTAKGASTDPL
                     QIAEYEGKKIFFEDLKPLMPVSDFEQVRHLRGQGLATAIKDALKAYLEKKIHDRVVKE
                     YFANEQQLRRFDHNRVAVLYLKVKYGKAGKGIYPASMDKIPLLPTEVYDHFFKMRNSL
                     ANVLWLKAAYTVVSEDSQAEEILAKLKKGEAFEKLAARYAISPKFIATARTHRIEGYN
                     DKLGPDARENRSYYDRLILDMAGRDVTNIEPYLGRDGIVVVRIYEVKRALDTIKLQDV
                     SWKVENDLRTKMLNAVYESDIHDAREKLKIQFNERVIKNLK"
     gene            complement(2746..3147)
                     /locus_tag="PLY93_05900"
     CDS             complement(2746..3147)
                     /locus_tag="PLY93_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP69046.1"
                     /translation="MRFTGSAINLPVQNITRTYNFYKQVFSEATVALEENIVSLRIGA
                     SLYFFMEHEAFETVLKPTQKTPEFTTEKFTALLSATVTTRDDAYAVLKRATDAGGTSC
                     GQAVHYAWGAAAYFEDVDHHLWEIIYRGSGI"
     gene            complement(3159..>4032)
                     /locus_tag="PLY93_05905"
     CDS             complement(3159..>4032)
                     /locus_tag="PLY93_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HRP69047.1"
                     /translation="KIFDKKARLIASATAESESKLLQITSHLKEDASKVFSFKNMVLR
                     NLTGDNTQLLVDLFQEIGRKEFKFIVASGWVFGALFGLIQMLIWWVYPGLWTLPVQGA
                     IVGYATNWIALVMIFRPMVEKRYLFIKYQGLFHRRQIEVSEKFSTMFAEHVLNGKNIL
                     EEVLYRRIGRTVVDAIQSGVTKELQALGVDNGSITAFEKASDARRRELAREMAAKLSA
                     TSQTMEKLISRSMNVKTTIYSRMKDLPAPDFEMLLRSAFQEDEWILIVLGAVLGASVG
                     LGQMFYMLFFSTPM"
     assembly_gap    4033..4042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4043..4384)
                     /locus_tag="PLY93_05910"
     CDS             complement(<4043..4384)
                     /locus_tag="PLY93_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HRP69048.1"
                     /translation="MEWFKQHAELIGVLLMPVTYGFIGWLTNVVALKMMFYPMKFWGV
                     PPYFGWQGIVPRKATGLALKVVNILSEKLLKIEEFFDKIPTDKLAEAYQPLIHENTPL
                     MTERVLELLPAD"
     gene            4536..>5576
                     /locus_tag="PLY93_05915"
     CDS             4536..>5576
                     /locus_tag="PLY93_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRP69049.1"
                     /translation="MTATNSATKFDSNALEKRIVILYSLGFIMPPSVWMVSLLLTGVI
                     ENISELVAIVTGPVMNLYILTYMILVVLSVRRSLKRIRGVDASSSPGVIDNAAKAARS
                     LPRSFILFMLVYCVLGPSSSFFRKDFISPTEYLLGELIAMPLIFLFAIPFLIAIVTAT
                     EKITIRVPLDNRHNFYSVGTKMFFFSSVSAFGGLLFLSIAAAALYTKRQVIGTDFDLF
                     IRLIIFFVLAIVIVFFNLAQLRFQISGPIKQLQERLGEISSAHADLTQRIHTHSRDEF
                     GFAAHAFNTFVQRLSHVLKDIREFSTLIQTASGLLSQTSAELFSASQTQKERLQSSQK
                     HITSVFTAVEENS"
BASE COUNT         1488 a         1280 c         1237 g         1561 t
ORIGIN      
        1 gggggccatt gttttgacag caggcggagt gaacgattat tttgtagctg tgcccgctgg
       61 ttcaagtttt aatcccctta caacgttacc tttgactgca acccctgcca ccacaggcgt
      121 tatcaagaac ctcatgcccg gcagttatga tgtgtaccgc acctgcgata aaaccaaatg
      181 tgcgaataca tccaccgctg gcacttgcga tgcaaccacg ctggggcaag atggctttcc
      241 tgaaaaagcg gtgccttgcg gtgcggcagt gacggtcacg aaaggcacaa cgacaagctt
      301 tggtgcgtgt gctgcagcgg cagggtgtcc atagagcctg tgcacaggct catagttcta
      361 tgatttttat cgacgatgta tcttaatttc catttctggc tcaatggatc ctctaaaagg
      421 gcagcgttcg catttcccca agcgcgcaga gcttacacat aattgggtgg ttgtcgacgc
      481 caaggataaa atcttgggtc gcgtcagtac acaaatcgcc tcacttctca tgggtaagaa
      541 taaagtcaat tatcaacccg gtcaactcgt gggcgatcac gtcgtcgtca tcaacgcttc
      601 gcaggtgaag ctttcgggta agaaagaaga aacgaaagag tatatctggc acagccgcta
      661 ttacggtgga atgaagaccc gtccctataa aacgcaaatg gaaaaagagc ctgagaaggc
      721 agtcttacta acagtcaaag ggatgctgcc taaaacaaaa tacggtagaa aattgctcac
      781 ccgtgttcgt gtttttaccg gcgcagaaca cagcttacaa ggccaaaaac ctgctgccgt
      841 ataggataaa aaatggaaaa tcagtcagaa agaaaagaaa ataaaaaagt agaaatggcc
      901 tcgcttgccg agtccatgac tcgccgcaaa gttgcattca agaaaaccgc ggacgacaaa
      961 gcccagacta aacgtgagcg caaaccaacc gatcaatttg ttggccgccg caaaaccact
     1021 gtggcgcgtg ttgcgttaaa aatgggttct ggtaaagtta ttatcaacaa tcgtgagtta
     1081 aacgaatact tttcgcgcgc tgatttacgc gccgctgtct tgcacccatt ccaagttgca
     1141 gagaaagtcg ggcaatacga tgttaaagtg tctgtatatg gcggcggcat ccaaggccaa
     1201 gcacaagcca tttcactcgg tattgcgcgg agtatcgacc gcaatgacga aacatcacat
     1261 tcaaaactta aagctgccgg cttgctcaga cgcgatccgc gtatggttga acgtaaaaaa
     1321 tatggcttgc ataaagcccg ccgtgcaacc cagttcagca agcgttaatc aaaaatggta
     1381 aactatcttt gcgcgcagtg cgcaaagata gtttatactt acttcagatt tttaataaca
     1441 cgttcgttaa attgaatttt aagcttttcg cgtgcgtcgt ggatatccga ttcgtagacc
     1501 gcgttgagca tttttgtgcg taaatcgttc tctactttcc atgaaacgtc ctgtaatttg
     1561 attgtgtcga gtgcgcgttt gacttcgtag atacgcacaa ccacaatacc atcgcggcct
     1621 aagtaaggtt cgatgttagt aacgtcacgc cccgccatgt ctaaaattaa acgatcgtaa
     1681 tagctgcggt tttcacgcgc atcggggcct aatttatcgt tatagccttc gatacggtgc
     1741 gttcgagctg tggcaataaa cttgggagag atcgcataac gtgcagcaag tttttcaaac
     1801 gcttcgccct ttttgagttt agctaaaatt tcttccgctt ggctatcttc ggaaacgaca
     1861 gtataagcag ctttgagcca caaaacgtta gcgaggctgt tacgcatttt gaaaaagtga
     1921 tcgtacactt cggtgggcaa taacggaatt ttatccatgg acgcgggata aatccctttg
     1981 cctgctttgc catatttcac tttcaaatag agcactgcta cgcggttgtg gtcgaagcgg
     2041 cgtaattgtt gttcgttggc aaaatattct ttgacgacac ggtcgtgtat tttcttttca
     2101 agatacgctt tcaatgcgtc ctttatcgca gtggcaagac cttggccgcg caaatgacgc
     2161 acttgctcga aatcggaaac gggcatcaag ggttttaaat cctcgaaaaa gattttcttc
     2221 ccttcgtatt cggcaatttg tagcggatct gtactcgccc cttttgcagt gagcgaggca
     2281 aattccttca tgaacgccgc ttcgttgtat tttaagccct cggcttgaaa aagcatttgc
     2341 caattaaact tttctttata ttttttttgc cagtggataa tcgccgcttt accaaagcgc
     2401 gcatgttctt gccgaaacat gtattcgtcg taagagggat cttctttcaa gcctttcttt
     2461 gtcgccagag caccaatgcc gtccaagtct gccaaatcgt ttaggatggc tcggtttctc
     2521 gcttgtggat tttcttcctc ttcaaattgg ttttggtaat aaacatcgcg cataaaataa
     2581 aatgtgccac gcgtgagctc tccgccctgg tagcgataga cgacgttaga gcaacccacg
     2641 gctaaaaaaa ggagtacaaa attaaacaga acaagtggga tttttttcga cgccagaatt
     2701 ctttttttga atggaaacat attggctacc tcggttgaca aacttttata taccagaccc
     2761 acgataaatg atttcccaaa ggtggtgatc gacatcttca aaatacgcgg cagcgcccca
     2821 cgcataatgc actgcttggc cgcaagatgt gccccccgcg tcggtggcgc gttttaatac
     2881 tgcgtatgca tcgtcgcggg ttgtgacggt ggcgctcaaa agcgccgtaa atttttccgt
     2941 cgtaaactcg ggcgtctttt gcgtgggttt taatactgtt tcaaaagcct cgtgttccat
     3001 gaaaaagtag agcgacgcgc caatacgtaa cgaaacgata ttctcttcga gggcaactgt
     3061 tgcttcgcta aaaacttgct tgtaaaaatt atacgtgcgt gttatgtttt gaacgggaag
     3121 attgatggcc gatcctgtaa aacgcattgt ggtaatttct acatgggggt tgaaaagaaa
     3181 agcatataaa acatttgtcc caagcccaca gaggcgccta acactgcgcc caaaacaatt
     3241 aaaatccatt cgtcttcttg gaacgccgag cgtaaaagca tctcaaagtc aggagcgggt
     3301 aaatccttca ttcggctata gatcgttgtt ttaacgttca tcgaacgact aatgagtttt
     3361 tccatcgttt gtgatgtggc tgaaagtttt gcggccattt ctcgcgccaa ttcgcggcgg
     3421 cgcgcgtcgg aagctttttc aaacgcggtg attgatccgt tatcgacgcc caaagcttgt
     3481 aactcttttg tcacgccgct ttggatggcg tcgacaacag tgcgcccgat gcgccggtag
     3541 agcacttctt ctaaaatgtt tttaccgttg agaacgtgct ctgcaaacat cgttgaaaac
     3601 ttctcagaaa cttcaatttg gcgacggtgg aaaagccctt ggtatttaat aaagagatag
     3661 cgtttttcca ccatgggtct aaagatcatc acgagggcaa tccaattggt ggcatacccc
     3721 acaatcgcgc cttgtacggg taacgtccac aagccggggt agacccacca aatcaacatt
     3781 tggattaatc caaaaagcgc gccaaaaacc catcctgaag cgacaataaa cttaaattct
     3841 ttgcggccaa tctcttggaa tagatcgaca agaagttgtg tgttatcgcc ggttaagtta
     3901 cgcaggacca tgttcttgaa gctaaagact ttgctggcat cttctttgag atggcttgtg
     3961 atttgtagta atttactttc agattctgcg gtggcgctgg caataaggcg tgccttttta
     4021 tcgaatattt tgnnnnnnnn nnatcggcgg gtaaaagttc gagcacccgc tctgtcatca
     4081 gcggtgtgtt ttcatgtata aggggttggt atgcctctgc cagtttgtcg gtaggaattt
     4141 tatcaaaaaa ttcttcaatt ttcaataatt tttcacttag aatattcact actttcagag
     4201 caaggccagt ggctttgcgt ggcacaatcc cctgccaacc gaagtaaggg gggactcccc
     4261 aaaatttcat gggataaaac atcatcttga gtgcaacgac atttgtaagc cagccaataa
     4321 agccgtacgt aacgggcatt aaaagcacac caataagctc agcatgctgt ttgaaccatt
     4381 ccatgggagc attttaataa ttacgacagg gagaatagaa aaactcattg agagttggca
     4441 caggctcctt tcccacttgg attaaaattt aattctttac tgtatactaa gaataatgga
     4501 gaatttctcc aatattctgt tatggggata aaaaattgac cgccacaaat agcgcaacaa
     4561 agtttgattc caatgcgtta gagaaaagaa ttgttattct ttactcatta ggatttataa
     4621 tgcctccatc cgtttggatg gtttcacttt tattgactgg cgttatcgaa aatatttccg
     4681 aattagtcgc catcgtcacg ggacctgtga tgaatctgta catcctcacg tatatgattc
     4741 tcgtcgtcct ctctgtgcgc cgatcgctca agcgcatccg tggagtcgat gcgagttcat
     4801 ctccaggagt aatcgacaac gccgcaaaag cagcgcgctc tcttcctcgt tcgtttatac
     4861 tttttatgct tgtctattgc gtgcttggcc ccagttcatc tttttttaga aaagatttta
     4921 tttcccccac agagtattta ctgggtgaac tgatagcgat gccgcttatc tttctttttg
     4981 ctataccgtt tcttattgcg atcgtcaccg ccacagaaaa aatcacaatt cgagtcccgc
     5041 ttgataaccg acataatttt tattccgtgg gaacaaaaat gttcttcttt tcatccgtga
     5101 gcgcttttgg tggacttctt tttctctcca ttgcggcagc agcgctttac acgaaaaggc
     5161 aggttatcgg aacggacttt gatttattca tacggcttat catttttttt gtattagcta
     5221 ttgttatcgt tttttttaac cttgcccaat tacggttcca gataagtggt cccataaaac
     5281 aattacaaga acggctgggt gaaatttcat cggcgcacgc agatctcacc caacgtatcc
     5341 acacgcacag tcgggatgaa tttggttttg ccgcacacgc ctttaatact ttcgtacaga
     5401 gactctccca cgttcttaaa gacatccgtg aattttcaac gctcatccaa acggcgtccg
     5461 gacttttgtc gcagacatct gctgaactgt tttccgcatc gcaaactcaa aaagaacgtc
     5521 tacaaagttc gcaaaaacat attacgtcgg ttttcactgc ggtggaagaa aactcc
//
LOCUS       DAOMPD010000134         5566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6156_length_5566_cov_2.589004, whole genome shotgun sequence.
ACCESSION   DAOMPD010000134 DAOMPD010000000
VERSION     DAOMPD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_5566_cov_2.589004"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLY93_05920"
     CDS             <1..454
                     /locus_tag="PLY93_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69050.1"
                     /translation="MRKFDYFMCCGISGIILPARDDVPMIGEYPRKDRVTYSFGPLRK
                     TDDYLAEIEAQLYDEHSDKKQFVIDKVMDQIYALGFDPRKTLPMAQVKYNPYAIYAAN
                     AYRNVIENVEQGFKNMKNKMAEQFSEKISQPVANRTRSFRDTIKNFWN"
     gene            complement(509..1651)
                     /locus_tag="PLY93_05925"
     CDS             complement(509..1651)
                     /locus_tag="PLY93_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69051.1"
                     /translation="MSSSVLSRVKPRPEGGFTHNWNDTILTNLGATADHFLRTFLSTR
                     MMFANIGTIPNVFQKEQRAKWDFYRNPEFIRRPETFFKHPEKTTPIVRGADPMGLGMD
                     EAIYEDIIFPSSMSPLNPSYDKEEIFSTPQASSIARHIRHKTNSNERPTLIVVHGYVL
                     DGYDFNAFLFEIERFFRLGFNILMYTMPFHGPRQKKNAAFSGDGFMVFDAGRIAETIR
                     WSIHDLTRYIDFITAHSKAPIGMMGFSLGGFHTALMASLDARLHFAIPVVPVITLFDV
                     ILDWQPSAAVIQAFLPLLNLSRESLNETLAVISPLSRPPLVPHERRFIIAGTADRMAH
                     PKHAQSLWKHWDEPRMYWFPGNHLVHFEKERFMREVAVFMRGMRLF"
     gene            1757..2338
                     /locus_tag="PLY93_05930"
     CDS             1757..2338
                     /locus_tag="PLY93_05930"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRP69052.1"
                     /translation="MLKTQKFLFIAALVFFIACNKAKRDDAQYLAFASQAMQDVMPQM
                     MQTVKQEVDKNGYASAVTFCSGFAAEFGKNKNVEWGKLATHDINAKSFRFRRISEKNR
                     NPNNRADAKQSVILAQWLKEGATPTLYRDGTRVTTMHPIKISMPMCLGCHGDAATVDK
                     KAIGEIKRLYPNDLATGYKLGDLRGAFVTEIEL"
     gene            2448..3446
                     /locus_tag="PLY93_05935"
                     /pseudo
     CDS             2448..3446
                     /locus_tag="PLY93_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802574.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     assembly_gap    3447..3456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3457..3612
                     /locus_tag="PLY93_05940"
     CDS             <3457..3612
                     /locus_tag="PLY93_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP69053.1"
                     /translation="SQCLQFFGGYGYMMEYPIAKAYLDCRVQTIYAGTSEIMREIVSK
                     NLGLAST"
     gene            complement(3635..3829)
                     /locus_tag="PLY93_05945"
     CDS             complement(3635..3829)
                     /locus_tag="PLY93_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69054.1"
                     /translation="MEKELHTAEVGYATARSRYRNFQGTLIDLQDTLVTYYRTRARQI
                     ETRLNLAYLLKIQALTNGLI"
     gene            complement(3835..4941)
                     /locus_tag="PLY93_05950"
     CDS             complement(3835..4941)
                     /locus_tag="PLY93_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP69055.1"
                     /translation="MKAKRRYFVFGLFYNLFATVLFSHWCVYPIEAQDIIISEADLIQ
                     RMNEVDPNILAAKLRIQEAEGKVKSARAGYFPSLELQALESIPGSFAGSNGLGGVGGV
                     MGSPYHTGFSAGVTATFTIFDFGRTLNASREADAAREGADLQSKITRIERLERALNIF
                     YNTIKFRELGILWVQKGKELSQVAREVGRYIATGQKSVVDRYLIRSRVEEVEREKIEF
                     NNRYNSGLEIIGELLDMKSEKLSLVSLAQLYTSGDMPKIEAWRSPYVLFMQAKKRAAE
                     YSLKRAQAGHMPEVRAYGSAGYMEGARLVPKENWGAGVAFALPLFEGFRVQGEVSAAN
                     SKVARVQKELDGAEKSMRATNIRLKKKLRRRANI"
     gene            complement(4938..>5566)
                     /locus_tag="PLY93_05955"
     CDS             complement(4938..>5566)
                     /locus_tag="PLY93_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP69056.1"
                     /translation="DVGSLSRAIQKQVINKTQFPEGYKAEIRGEIESMQDAVKQMGNG
                     FLLSVLLVYLILVVQFRSFILPAIMMITVPLGLVGVVLMLFSTNTYFSLQAGIGTIFL
                     IGIAVANGVLLVEFLLHKIEHDKLSVDEGILEGAKARLRPILMTALASILGLTPMAIG
                     LGKGSEANIPLGRAVIGGQFLATLLTLFVVPTIFRAVYNRFIYKEGVQ"
BASE COUNT         1564 a         1344 c         1246 g         1402 t
ORIGIN      
        1 catgcgcaag tttgattact ttatgtgctg cggcatttca ggaattatct tgcccgcgcg
       61 cgatgatgta cccatgattg gcgaatatcc gcgtaaagat agagtcacgt attcgtttgg
      121 accgttacgt aagaccgacg actacttggc ggaaattgaa gcacaactct acgacgaaca
      181 ctcggataaa aagcaatttg tcatcgataa agtgatggat caaatttacg cgcttgggtt
      241 tgacccgcgc aaaaccttgc cgatggcgca agtgaaatat aacccgtatg cgatttatgc
      301 agcgaacgcg taccgcaatg tgatcgagaa cgtcgagcaa ggcttcaaga acatgaagaa
      361 taaaatggcc gagcagttta gcgaaaaaat tagtcaaccg gtggcaaatc gtacgcgtag
      421 tttccgcgac acaattaaaa atttctggaa ctaaagggtg gactctcagc cttgaacttt
      481 ggggacactt accaaattct ttactctatt aaaaaagacg catgccgcgc ataaagaccg
      541 ccacttcgcg cataaaccgc tctttttcaa aatgcactaa atgatttcca gggaaccaat
      601 acatccgtgg ttcgtcccaa tgcttccata aactttgtgc atgcttgggg tgcgccatgc
      661 gatctgcagt gccagcaatg ataaagcggc gttcgtgcgg cacaagcggg ggacgcgaaa
      721 gcggagagat gacagcgagg gtttcattca aactttcacg cgacaaattc aagagcggca
      781 aaaatgcttg gatgactgcc gcgctcggct gccaatctaa aattacatcg aagagggtga
      841 tgacaggcac cacgggaatc gcaaaatgca atcgcgcatc taaagatgcc atgagcgcgg
      901 tgtgaaatcc tcctaacgaa aaacccatca tcccgatggg ggctttagag tgcgcggtaa
      961 taaaatcgat gtagcgcgtt aaatcgtgta tcgaccaacg gattgtctct gcgatgcggc
     1021 cagcatcgaa caccataaat ccgtcaccgc tgaacgccgc attttttttc tgacgcggcc
     1081 catggaacgg catagtgtac atcagaatat taaaacccaa ccgaaaaaag cgttcaatct
     1141 caaaaaggaa tgcgttgaaa tcataaccat cgagcacgta accgtgaacg acaatcaatg
     1201 ttggccgctc attgctattg gttttgtggc ggatgtgtct tgcaatcgac gaggcttgcg
     1261 gcgtggaaaa aatttcttct ttatcgtaag aaggattaag aggcgacata gaactgggga
     1321 aaataatatc ttcgtaaatc gcttcatcca tccccagccc cattgggtcg gcgccacgaa
     1381 caatcggtgt tgtcttttct ggatgtttaa agaatgtttc cggtctgcgg ataaattcgg
     1441 ggttacgata aaaatcccat ttagcgcgct gctctttttg aaacacgttg gggatcgtgc
     1501 cgatattggc gaacatcatg cgcgttgaaa gaaaggtgcg caagaaatga tcggcggtgg
     1561 cgcccaagtt tgttaaaatc gtgtcgttcc agttgtgtgt gaaaccacct tcggggcgcg
     1621 gcttaacgcg gctcaatacg gaagaactca taatctataa ggaataaatg ttaaaagcga
     1681 taaagcgtct ataaattaaa gaatctttta cgttcatgag ttgccgccat gacttattcc
     1741 tctcttctgc tcggttatgc ttaaaacaca aaaattttta tttattgccg cgcttgtatt
     1801 ttttatcgct tgcaataagg cgaagagaga cgatgctcag tatcttgcgt ttgcaagcca
     1861 ggcgatgcaa gacgtgatgc cgcaaatgat gcaaaccgtg aaacaagaag tagacaaaaa
     1921 cgggtatgcg tccgcagtca ctttttgctc tggctttgca gccgagtttg gcaaaaataa
     1981 aaatgtcgaa tggggaaaac ttgccacaca tgacatcaac gcaaaatctt ttcgcttccg
     2041 ccgtatctct gaaaaaaacc gcaatcccaa caaccgtgcc gatgccaaac aaagtgtaat
     2101 tcttgcccaa tggctcaaag aaggtgcaac ccccactctt taccgcgacg gtacccgtgt
     2161 aacgacaatg catcctatta aaattagtat gcccatgtgt ttaggctgtc atggcgacgc
     2221 tgcgaccgtc gacaaaaaag ccatcggtga aattaaacgc ctttacccaa acgatttggc
     2281 caccggctac aagttggggg atttacgcgg cgcgtttgtc acggagattg agctttagcg
     2341 acttctacag gctcgtaatc tctttatgag ttttcgttta cgtcaggtct ttatgttctc
     2401 aaaagacgct gtaacacttg ctacaaaaaa agaattgagg aatagttatg actaaaagat
     2461 taaaacgcac actacctttc acccccgaac acgacgagtt ccgtaaaatg gtcgctgact
     2521 ttcttgcgaa agattgcgtc ccccgccacg aagaatggga aaaagccggc gaagtcacgc
     2581 gcgacgtgtg ggaaaaagca ggtcaactcg gcatgatttg ccccaatttc cccgaagaat
     2641 acggcggctt aggactcgac tttctgtata atgttattgt gattgaagag cttgccaaag
     2701 tagcttcatc cggttttttt atttcactcc acgccgacgt gattgcgcct tacattttgc
     2761 attacgctaa cgacgaacaa aaaaagcgtt ggcttccagg agtgatcgac ggtacaaaaa
     2821 ttttgtcgat cggtatgact gaacctggca caggttctga cttagccgcg attacgacaa
     2881 aagccgaaga caaaggcgat tattatctcc tcaatggttc aaaaactttt atctctaacg
     2941 gctacctctc cgatttatgc atcgttgtgg cgcgcactgg cgaaggccaa ggcggtattt
     3001 cgctattgat ggtcgaacgc ggcatggaag ggttcgagcg tgggcgtaaa ctcaaaaaga
     3061 ttggtcttca cgcacaggat acatccgaat tacatttcaa cgatgttaaa gtacccaaga
     3121 caaacgtcat cggtcgcttg aacgcaggct tccgttatct catgcagtcg ttagcgcaag
     3181 agcgtttagt gcgatttcca acatcgcttc ggcagaaact gtgctcgatc aaaccatcaa
     3241 gtattgcaac gagcgcaaag cattcggtaa acccatcggc gcatttcaaa acacgcgctt
     3301 caaacttgtc gatatgtacg tcgagcaaga agccgctcgc caatacctcg accgtgtggt
     3361 cgaattgcac atgaagggcg aagcgacacc cgcgatggca tcggcgacaa aactacaatg
     3421 ctctgaattg ttgcagtcgc atgtttnnnn nnnnnntcgc aatgcttaca gttctttggc
     3481 ggctatggct acatgatgga ataccccatt gcaaaagcat acctcgattg ccgcgtgcaa
     3541 acgatttacg caggcacgtc ggagattatg cgcgaaatcg tctcgaaaaa cttgggtctc
     3601 gcgagcacat aaaatcagcg ctctctcccg ctttttatat taagccgttc gtgagagctt
     3661 gaatttttaa gagatacgcc aaattgaggc gtgtctctat ttggcgtgcg cgggttcgat
     3721 agtacgtgac taacgtgtct tgcaaatcga taagcgtacc ttgaaaattg cggtaacgcg
     3781 agcgcgcagt tgcatacccc acttccgctg tatgcaactc cttttccata agtttcaaat
     3841 gttcgcgcgc cgtctcaact tcttttttaa gcgaatgttt gttgcacgca tacttttctc
     3901 tgcaccatcg agttcttttt gtactcgtgc aacttttgag ttcgctgcac tcacttcacc
     3961 ttgtactcgg aaaccttcaa aaagaggcag cgcaaaagca acgcctgccc cccagttctc
     4021 tttgggtaca agtcgtgcgc cttccatata accagccgaa ccataagcgc gcacctctgg
     4081 catgtgacct gcttgtgcgc gtttgagact atattccgca gcgcgtttct ttgcttgcat
     4141 aaataataca taggggctgc gccacgcttc tattttaggc atgtcgccgc ttgtataaag
     4201 ctgcgcaagt gaaacaagag aaagtttttc agatttcata tccaacagct cgccaataat
     4261 ttcaagcccg ctattgtacc ggttattgaa ttcaattttt tcacgttcga cttcttcgac
     4321 acgcgaacgt atcaaataac gatcaacgac agatttttga ccggtagcga tataacgccc
     4381 cacttcacgc gccacttgag aaagctcttt tcctttttgt acccagagaa tccccagctc
     4441 ccgaaattta atcgtattat aaaaaatgtt aagcgcgcgc tccaagcgtt caatccgtgt
     4501 aatcttactt tgtaagtcgg ctccttcgcg ggcggcatcg gcttctctgc tcgcattcag
     4561 cgtgcgacca aaatcaaaaa tcgtgaacgt tgcggtaacc cccgctgaaa aaccagtatg
     4621 gtagggtgaa cccatgactc caccgacgcc gcccagaccg ttactccctg caaatgaacc
     4681 aggaattgat tcgagcgctt gaagctccaa agaaggaaaa tatcccgctc ttgcgctttt
     4741 tactttacct tcggcctctt gaatgcgcaa cttagccgcc aagatatttg gatcaacctc
     4801 attcatgcgc tggattaaat cagcttcgct aataataatg tcttgtgctt caatgggata
     4861 tacacaccaa tgggaaaata gtactgtagc aaagagatta tagaacaacc caaaaacaaa
     4921 atagcgccgt ttggctttca ttgaacacct tctttatata taaatcgatt ataaactgct
     4981 ctgaagattg taggcactac aaagagggtt agtagcgtcg ctaagaattg accaccaatc
     5041 accgcacgcc ccaaaggtat gttcgcttcg gaacctttgc ccaaaccaat tgccatgggg
     5101 gtgagaccca aaattgacgc aagcgcagtc atcagaattg ggcgtaaacg cgctttggcg
     5161 ccttctaaaa ttccttcatc cacagacaac ttgtcgtgct caattttgtg gagcaaaaac
     5221 tccaccaaga gtacaccgtt ggccacggca atgccgatga gaaaaatagt accgatgcct
     5281 gcctgcaaac taaaatatgt attggtggaa aagagcatca atactacccc cacgagtccc
     5341 aatggcacgg taatcatcat aatagcaggc agaatgaacg aacgaaactg tacaaccaag
     5401 atgagataca ctaaaagcac cgagagcaaa aaaccattgc ccatttgctt taccgcatct
     5461 tgcatcgatt caatttcccc acgaatttcc gctttatagc cttcggggaa ctgggtttta
     5521 ttgataactt gtttttgaat tgcgcgcgaa agcgaaccca cgtcac
//
LOCUS       DAOMPD010000135         5562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6162_length_5562_cov_3.093154, whole genome shotgun sequence.
ACCESSION   DAOMPD010000135 DAOMPD010000000
VERSION     DAOMPD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6162_length_5562_cov_3.093154"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1061)
                     /locus_tag="PLY93_05960"
     CDS             complement(<1..>1061)
                     /locus_tag="PLY93_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69057.1"
                     /translation="AGHCDEVIVEIQKGNIISVSDNGRGIPVGIHPKVGVSTVEVVLT
                     KLHAGGKFGDGAYKVSGGLHGVGVSVVNALSEWVEVDVHVDGKQHHIHFDRGKPKAPL
                     KVKGASKKRGTTVTFLADHEIFDSLEYRYETLCARLRELAFLTKGLKIVIRDLRDKNP
                     KEQIFQYKGGIVEFVKMLTEKKNPLQKKPIYAIEQKDGIQVEFAIEYCDTYSEQIFTF
                     ANAIHTREGGTHLEGFRTALTRAINEYKKKLGYDKKVEEPLTGDDVREGLTLVLAILI
                     SNPQFEGQTKMKLGNGEVKGIVTSVVYEKLCEYFDENPADAKRVIEKCMQAALARIAA
                     RKAKELVRRKSALEGAGLP"
     assembly_gap    1062..1071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1072..1318)
                     /locus_tag="PLY93_05965"
     CDS             complement(<1072..1318)
                     /locus_tag="PLY93_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRP69058.1"
                     /translation="MAKSETPQKSDKAVPKKEEEQAIHGLVSETYDASKITVLEGLEA
                     VRKRPGMYIGSTDLPGLHHMVYEVVDNAIDEALAGHAT"
     gene            1491..3038
                     /locus_tag="PLY93_05970"
     CDS             1491..3038
                     /locus_tag="PLY93_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRP69059.1"
                     /translation="MTPHHQRSSRTLILITIIILGAMSVAILGIHLFMQNNAKSVIRR
                     TGQTLMNRTVQMFVKATVAFEKDYATGKSENERKAIMDKWNSAILTFIRANTNDYGTN
                     APRVRFVGDEKLFGLKPLGGDATALTLPFEKRAAEKLAQNSNSAYEESDASHLRIAMA
                     LPANVNEGCARCHSLPLNTTKLLGTANVYIPLGEYQKHSRVNSVVVAIGIALLLGFVL
                     LVLLIFLRRNILKPVEYIADTAETIAKGNLTQEVIVFGTPEMRDALTQLSKLQQEFTH
                     TFRDIQQHSESVMSAAGHIAATAQDLSRTASEGGEQNASDANIVELIRLIESNATGAK
                     ETDTLAQESSALAEQGSQTFRETFTALEAIVNKISIIQEIADQTNLLALNATIEAARA
                     GEHGRGFAVVASEVSKLAEMSRAATNEINDVAHRSLNHSEAAQKLLAKILPSIKQTTE
                     NVRGIHTRSEAQTKHVGEIRAVLDAMSKITANIASASEQLAASAEELTAQSETLDERL
                     SFFQIKE"
     gene            3041..4222
                     /locus_tag="PLY93_05975"
     CDS             3041..4222
                     /locus_tag="PLY93_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRP69060.1"
                     /translation="MNRKFTKYAGALLCLVLIQCTPDKETKASSITLDAPEKGYWFLS
                     EKFPYQFIRIELYKNHIEGLPSLTMEFRVKAESKDATWETDASTVRLSFKKPTAGNVS
                     LTPVYDNTHYKGRYTKEKLLLPLMEVKLGSERRIMHYTESDAEMDRLLREKNKLKLDV
                     VWGPTQKEVIDAMFRFTHPTRNDVIFDLGCGDARILIAAAQRFGAHGVGYDLDPKLIE
                     KGWSDAKRARVDQLLTLKTENLFAADLSSATIVAIYLSDRINAKLKPKFFRELSPGTR
                     LVSHNWHMGDWQFDGKEVMEDKTRVVYFWVMPANFSGTWKDETGATFKIDQHYQFIDI
                     ESARNSQKPLKSLRVRGLFTASTPLGVATLDGNNLRWQKKGTPDVLWIREKDSAVPFS
                     L"
     gene            complement(4474..4890)
                     /locus_tag="PLY93_05980"
     CDS             complement(4474..4890)
                     /locus_tag="PLY93_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRP69061.1"
                     /translation="MNAVLLDTGFLVALFDRHDSKHGECAEILASLDCALITCETVIA
                     ESAYLLRRCNGAAEQIMKNIEKRVFEIPWRLIGNESRLKKLFLKYGSQKINLSSACLI
                     LMAEDFSTGDILTLDSNFLFYRWNKTKRFRIFPHIT"
     gene            complement(4887..5126)
                     /locus_tag="PLY93_05985"
     CDS             complement(4887..5126)
                     /locus_tag="PLY93_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69062.1"
                     /translation="MAKTITARIDDTTEKQLARLALKLHQSESQVIRRSIESLAALEL
                     NTKRKPKKIFYGLGEYDSGIADLGTNKAYLHDFGR"
     gene            5218..>5562
                     /locus_tag="PLY93_05990"
     CDS             5218..>5562
                     /locus_tag="PLY93_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP69063.1"
                     /translation="MPEAIMEKESASVRLAVAKTKKLYIGGDFPRSESGRSFPLVAKT
                     TGKFYANIAQASRKDARNAVEIARKAATSWAKKTAYNRGQILYRMAEMLEARAGEISA
                     ELVHVSGVKAADA"
BASE COUNT         1496 a         1365 c         1283 g         1408 t
ORIGIN      
        1 ccggaagacc agcgccttcg agcgcactct tacgccgcac gagttctttg gctttacgcg
       61 cagcgatgcg tgcgagcgcg gcctgcatac acttttcaat aacgcgctta gcgtcggcgg
      121 ggttttcgtc gaaatactcg catagttttt cgtacacaac cgaggtaaca attcctttta
      181 cttcgccgtt acccaacttc atttttgtct gaccctcaaa ctgtgggttc gagattaaaa
      241 tcgcgagcac taaagttaat ccttcgcgca cgtcatcgcc ggttaacggt tcttcaactt
      301 ttttatcgta gcctaatttt tttttgtatt cgttaatcgc acgggtgagt gcagtgcgga
      361 agccctcgag gtgtgtgccg ccttcgcggg tgtggatggc gtttgcgaac gtaaaaattt
      421 gttcagagta tgtgtcgcag tactcaatcg caaactcgac ttggataccg tctttttgct
      481 cgatggcata gatcggtttt ttttgcagag gatttttttt ctcggtgagc atcttcacaa
      541 actcaacgat accaccttta tactgaaaga tttgttcttt cggattttta tcccgtaagt
      601 cacggataac aattttaaga cctttggtga ggaatgccaa ttcgcgtagc cgtgcgcaga
      661 gtgtttcgta acggtattcg agtgaatcaa aaatttcgtg gtcggccaaa aatgtgactg
      721 tggtgccgcg ctttttactc gcgcctttta ctttgagcgg cgcttttggc ttaccgcgat
      781 cgaaatggat gtggtgttgc ttaccatcga catgaacatc aacctccacc cattcggaaa
      841 gcgcattaac caccgacact cccacaccgt gcaaaccgcc cgaaactttg tacgcgccgt
      901 cgccgaattt gccgcccgcg tgtaacttgg tgagcaccac ctcaaccgta gaaacaccca
      961 ccttggggtg aatacccacg ggaataccgc ggccattgtc agagacgctg atgatatttc
     1021 ctttttggat ttcgacaatc acttcgtcac agtggccggc cnnnnnnnnn ncggtggcgt
     1081 gaccggcgag cgcttcgtcg atggcgttgt cgacgacttc atacaccatg tggtggagcc
     1141 ccggcaaatc cgtcgaacca atgtacatcc ccgggcgctt gcgtactgct tcgaggcctt
     1201 ctaaaacggt aattttagac gcatcgtacg tttctgaaac aagcccatgg atggcttgct
     1261 cttcttcttt tttgggtacc gccttatctg atttttgggg agtctctgat tttgccatat
     1321 taaggaagcc atattagcaa atacagacat tatgtcactt cagaacggat ttactcacga
     1381 gcctctgagg agtctgtgcg cacaggctct tatactccga aagtatcaaa atagttgcac
     1441 gatcatccgc aatttgacga taataaaaga aaataaccaa aggacctatc gtgacccccc
     1501 accaccaaag aagtagccgc actctcattc tcatcaccat tattatttta ggcgcgatgt
     1561 ctgtcgctat tttagggatt catctcttta tgcaaaacaa tgcaaagtcg gtaattcgtc
     1621 gcaccggcca gacactcatg aaccgcactg tgcaaatgtt tgtcaaagcc accgtcgctt
     1681 ttgaaaagga ttatgcaacc ggcaaaagcg agaatgagcg caaagcaatt atggataaat
     1741 ggaactcagc cattttaacg ttcatccgag caaataccaa cgactatggc acgaatgcac
     1801 ctcgcgtgcg ttttgtcggt gacgaaaaac tctttggcct taaacctttg ggaggcgatg
     1861 ctactgcatt gactttgcct ttcgagaaaa gggcggcgga gaagttagct caaaattcga
     1921 atagcgctta cgaagaaagc gatgcgtcgc acttacgtat cgcgatggca ctccccgcaa
     1981 acgtcaatga gggttgtgcc cgctgccaca gccttccctt aaacacgaca aaactattgg
     2041 gtactgcgaa tgtgtatatc ccccttggcg aataccaaaa acattcacgc gtcaatagtg
     2101 ttgtcgttgc tattgggatt gctttactct tgggttttgt gcttttggtt ctacttattt
     2161 ttttacggcg caatattctt aaaccagtgg agtatatcgc cgacactgcc gaaaccatcg
     2221 ccaaaggaaa tctaacccaa gaggtgatag tgttcggtac tccagaaatg cgcgatgcgc
     2281 tcacacaact ttcaaaattg caacaagagt tcacccatac tttccgtgac atccaacagc
     2341 attccgaatc ggtgatgtca gcggcgggac atatcgccgc caccgcgcaa gatttatcgc
     2401 gcaccgcctc tgaagggggc gaacaaaacg cttcggacgc caacatcgtt gaactcatcc
     2461 gtttgattga aagcaacgcc accggcgcca aagaaaccga cacgttggcg caagagtcat
     2521 cggcgctcgc cgaacaaggc agccaaactt ttagagaaac tttcactgcg cttgaagcaa
     2581 tcgtaaataa aatttctatc atccaagaaa ttgccgacca aaccaattta cttgctctca
     2641 acgccaccat cgaagcggcg cgtgcgggcg agcacggccg tggttttgcc gtggttgctt
     2701 ccgaggtgag caagcttgct gagatgagcc gcgccgccac gaatgaaatc aacgacgtgg
     2761 cgcaccgatc gttaaaccat tcggaagcgg cacaaaagct cttggctaaa attttgccgt
     2821 ccatcaagca aaccacggag aatgtgcgcg gcatccacac ccgttccgaa gcgcaaacga
     2881 aacacgtcgg cgaaatccgc gcagtgctcg atgccatgtc gaaaattacg gcgaacatcg
     2941 cgtcggcttc cgagcaactc gccgcttctg cagaagaatt gacggcacaa tcggaaacgt
     3001 tggacgaacg gctttctttc tttcagatca aagaataaca atgaacagaa aattcacaaa
     3061 atacgcgggt gcacttctat gccttgtgct tatccagtgc acacctgaca aagaaactaa
     3121 agcaagctcg attacacttg acgcacctga aaagggatat tggtttttat ccgaaaaatt
     3181 tccctatcaa tttatccgca tcgagcttta caaaaatcat atcgaaggtt tgccgagcct
     3241 cacgatggaa ttccgtgtga aagccgaaag taaagacgct acatgggaaa ccgacgcgag
     3301 tacggtgcgc ctttcattta aaaaacccac tgcggggaat gtgtcgttaa caccggtgta
     3361 cgataacacc cattacaaag gccgttatac aaaagagaag ttattgttac ctcttatgga
     3421 agtaaaactg ggtagtgaac gtcgtattat gcactacacc gaaagcgacg cagagatgga
     3481 tcgccttttg cgtgaaaaaa acaaactgaa actcgacgtg gtgtggggac caacgcaaaa
     3541 agaagtcatc gatgcgatgt tccgtttcac gcaccctaca cgcaacgacg ttattttcga
     3601 cttgggttgc ggcgacgcgc gtatcctcat cgccgcagca caacggtttg gagcgcatgg
     3661 cgtgggttac gacttagatc ccaagttgat tgagaaaggt tggagtgacg ccaaaagagc
     3721 gcgagtggat caactactca ccctcaaaac tgaaaatctt tttgctgccg atttgtcgag
     3781 cgcgacaatt gtcgctatct atttaagcga ccgcatcaat gcaaagctga agcccaaatt
     3841 ttttagagaa ctttctcctg gaacgcggct ggtctcacac aactggcaca tgggcgattg
     3901 gcagtttgac ggtaaagaag tcatggaaga taaaacccgt gtggtatatt tttgggtgat
     3961 gcccgccaat ttttccggca catggaaaga cgaaacaggc gccacattca aaatcgacca
     4021 gcactatcaa ttcatcgaca tcgaatcggc gagaaattcg caaaagccgt taaaaagttt
     4081 acgagtgcgt gggctcttca ccgcaagtac acctttgggt gttgctactt tagacggtaa
     4141 caatttacgc tggcaaaaga aaggcactcc cgacgtgctt tggattcgag agaaagatag
     4201 cgcagtgccg ttttcgttgt aatttagtga ctgtttactc aaagggtgtt tttactcaaa
     4261 attttcccgg ttttgactct tccagcactc gcggtgcata gttaccccct aatgcgacat
     4321 aatccaagaa aatcggcata gttaccccct gatgcgacat aatccaagaa aatcagcata
     4381 gttaccctgg cagtttgcgt acttaaaact tatagaagtg ttgtgccagt gttgcatacg
     4441 actgattgga tgcttcggtt tttcgataat cttttaggtt atatgtggaa aaatacgaaa
     4501 tcgtttcgtc ttgttccacc gataaaaaag aaaattcgaa tctaaagtga gtatatcgcc
     4561 agttgaaaaa tcttcagcca tgaggattaa acaagcgctg gataaattaa ttttttgcga
     4621 accgtacttg agaaagagtt ttttgagacg cgattcgttg ccgataagtc gccacggaat
     4681 ttcaaaaacc cgcttttcta tatttttcat aatttgttct gcggcaccat tacaacgacg
     4741 caataagtat gcggactctg caataaccgt ttcgcatgtg ataagcgcac aatctaagga
     4801 agctaaaatt tcggcgcatt caccgtgctt cgagtcgtgg cgatcgaaca acgcgaccag
     4861 aaagccggtg tcgagtaaga ccgcgttcat ctaccgaagt cgtgcaaata agccttgttc
     4921 gtacccagat cagcgatacc cgaatcatat tcccccaacc catagaagat ttttttgggc
     4981 tttcttttcg tattcagctc aagcgcggcg agtgattcta tagaacgacg aatgacttgc
     5041 gattcgctct ggtgcagttt taaggcaagt cttgccagtt gcttttccgt tgtatcatcg
     5101 atgcgggcag taattgtttt agccatgtat tacaaataca ggtgcgtatt acggtttgtc
     5161 aaattctttt caaagcaaaa tgatttgacg gggtaaaccc aaccacaacc aacccccatg
     5221 cccgaagcga ttatggaaaa agaaagcgcg agtgtacgcc tcgccgttgc gaaaacaaaa
     5281 aaactttata ttggcggcga ttttccgcgc tcagagtcgg ggcgttcgtt tccgttagtc
     5341 gctaaaacca ccggtaaatt ttatgccaat atcgcacaag ccagccgcaa agacgcgcgt
     5401 aacgctgtcg agattgcgcg caaagcggca acaagttggg caaagaaaac cgcgtacaac
     5461 cgaggtcaaa ttctctaccg catggcagag atgctcgaag cgcgcgccgg tgaaataagt
     5521 gcagaactcg tacacgtctc tggtgtcaag gctgccgacg cg
//
LOCUS       DAOMPD010000136         5562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6163_length_5562_cov_2.739967, whole genome shotgun sequence.
ACCESSION   DAOMPD010000136 DAOMPD010000000
VERSION     DAOMPD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6163_length_5562_cov_2.739967"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLY93_05995"
     CDS             <1..366
                     /locus_tag="PLY93_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRP69064.1"
                     /translation="RQIPRRDMGLIIEARMREMLELVDHELLRSGRKNILAGGIIFTG
                     GGAQLEGLVGLAEEVLGLGASVSYPKGLLGIADKVASPPFSTATGLLLYQARYGEVIT
                     QKNKRNLFGKMKSWLQKNL"
     gene            376..1551
                     /gene="ftsZ"
                     /locus_tag="PLY93_06000"
     CDS             376..1551
                     /gene="ftsZ"
                     /locus_tag="PLY93_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRP69065.1"
                     /translation="MLAFEEKKKSPATIKVVGVGGGGMNAIERMVTMNLEGVELIAMN
                     TDEQVLERSHAPIKVALGAKLTRGMGAGGIPDVGAQAAMEDREKIMNVLKGADMVFIT
                     AGMGGGTGTGAAPIVAEVARELKALTVGVVTLPFLVEGEKRMTYAKQGQSNLRERVDT
                     LITIPNEAIFKVIDKKTSVGAAYKLIDDMLYRAVRGISNIINSTGFVNVDFADVRTVM
                     GQNGDAVIGLGEGTGESRVAEALQSAIHHPLLNGKTIDGAQAVLINVVGGSDFALYEY
                     HEIQKQIHEQVSKDAVIIVGFTEDETKDNHVSITVIATGYRRTSTADTRPQAPTQQPP
                     HAGQPFRPQILPRTGTEDPNYRSPTQQGLDFPRVDASVQAAAYDIPTFLRKMPKIDG"
     gene            1551..1892
                     /locus_tag="PLY93_06005"
     CDS             1551..1892
                     /locus_tag="PLY93_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69066.1"
                     /translation="MPWPYLFFNFVAFTLLYSGHVVAGITTLVTEAAFFVLDARRAGR
                     KLLFPVLLGLLLGFSLFILRLPYLSLTNHNFESGEAVVTAVSRKSVVIARVDKIKLRL
                     IGIPKEKLPAN"
     gene            2082..3383
                     /locus_tag="PLY93_06010"
     CDS             2082..3383
                     /locus_tag="PLY93_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRP69067.1"
                     /translation="MQKNKSLVAAFLLGDTEDLSASELEAFRSMGLMHLFAVSGLNIA
                     LLFALLYIPFRLIGQKAIGTALGYTVAVLFLLLLDFPVPLLRAWVFMTIALFMRVLDR
                     RIPSWTSLFLTAIFVEILFPLSTFSMSFILSFGVTAAIFIFYEPFYFCFASSKKYLKL
                     WNEHLALTLAAGLPALLLTYFLFGQANPLSLLYNLLLVPFSGIYLFAALIFLFFDPAQ
                     YIFLPLDTLYLEFAEWHQTYISANFPLADKTAQQISLAFVATLLLLLYITKIRRRLWS
                     AKRFVRVVIPLLVVILLLPYFLVEYPKNAFYAIPNKVWIYSDAQLTTVGASVFSPPPK
                     EPKICFPVHHRHEIALDKRMPKEILQHGNACYIFSGRMQPEKWRSHILKPCSQLYLLH
                     GKDVTPNVAEWKSLFVLFGYSGAVALRNYFKWYGDGIVDCM"
     gene            3652..4743
                     /locus_tag="PLY93_06015"
     CDS             3652..4743
                     /locus_tag="PLY93_06015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRP69068.1"
                     /translation="MDFIDLKTQQARIKTQIDANIQKVLAHGQYIMGPEITEIEKRLA
                     TYTGAKHCLTVANGTDALLIALMALGVKAGDEVITSPFTFIATGEMIALLGAAPVFVD
                     IDPKTYNIDPTKIEAAITPKTKAIMPVSLYGQCADFDAINVIAQKHNLPVIEDAAQSF
                     GAEYKKKKSCNLSTIGCTSFFPSKPLGCYGDGGAVFTNDDELAKKMGYIRLHGQDKRY
                     HHPIIGVNGRMDTLQAAILLPKLDIFPEEVTLREKIGARYSSRLAAHGITTPYIEPYN
                     LSVYAQYTVLVDNRETVQKKLQEHGIPTAVHYPIPLNLQPVFAHLNQPKGSFPIAERI
                     AERVVSLPMHPYLDEATQDKIITAVAAAV"
     gene            4747..5106
                     /locus_tag="PLY93_06020"
     CDS             4747..5106
                     /locus_tag="PLY93_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69069.1"
                     /translation="MQTLLVVLLSLLALLSGAALHYFFSLRRALKSVRYEMRELRQLQ
                     SSPYNDAVFDEPIIRMLVEVKDPIRLAHRKSPLAKIASPTAPNLVAKKVYEQVMQETR
                     EQLKGREVEANVSLIVL"
     gene            5107..>5562
                     /locus_tag="PLY93_06025"
     CDS             5107..>5562
                     /locus_tag="PLY93_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69070.1"
                     /translation="MTIELLVFFALVGLILCTLFFLFLRKKEAQVRRFRREIFLKKTA
                     LKGAAFAQLLKAENTQLIAHGIDQSALFVRGGHRLVSDVTFGLMRLIPATREQARAAK
                     DVHDFISDSLYGALRIINEQIGGSIAQNIKMVKPKVKTKTTKIKKNSKNN"
BASE COUNT         1409 a         1359 c         1315 g         1479 t
ORIGIN      
        1 cggcaaatcc cacgccgtga catgggcctc atcattgagg cgcggatgcg cgaaatgctc
       61 gaactcgtcg accacgaact gttgcgttcg ggcagaaaga atattttagc aggtggtatt
      121 atattcacag ggggcggcgc acagcttgaa ggtcttgtcg gtctagccga agaggtttta
      181 gggttgggtg catctgtctc ttatccaaag ggcttgttgg ggattgccga caaggttgca
      241 tcacccccgt ttagcacggc gactggatta ctcttgtatc aggcgcgcta cggtgaagtg
      301 attacacaaa aaaacaaacg caaccttttt ggcaaaatga agagttggct gcagaagaac
      361 ctataggaga tagaaatgct ggcatttgaa gaaaaaaaga aatcccccgc aacaattaaa
      421 gtggtgggag tcggtggcgg cggtatgaac gccattgaac gtatggtcac gatgaacctc
      481 gaaggtgtcg agctcatcgc aatgaacacc gacgagcagg tgttggagcg ctcgcacgca
      541 ccgattaaag tagcgttggg agcaaaactc acccgtggta tgggggcagg cggaatcccc
      601 gatgtgggcg cgcaagcggc gatggaagac cgtgagaaaa ttatgaatgt cctcaaaggc
      661 gcggacatgg tttttataac cgctggcatg ggcggtggta caggaaccgg tgcagctccc
      721 attgttgcag aggtggcgcg cgaactcaaa gcgctcaccg tgggcgtggt gacactacct
      781 tttctcgtcg aaggcgagaa gcggatgact tacgctaaac aggggcaatc caacttacgc
      841 gaacgcgttg acactctcat cacgatcccc aacgaagcga ttttcaaagt catcgataaa
      901 aaaacttcgg taggcgcggc gtataaactc atcgacgaca tgctctaccg tgcagtgcgg
      961 ggcatcagca acatcatcaa ctcaaccggt tttgtgaatg tcgacttcgc cgacgtacgt
     1021 accgtgatgg gacaaaacgg cgatgcggtg attggtttag gcgaaggcac cggcgagagt
     1081 cgtgtggccg aagcgttgca aagcgcaatc caccatccgc tcttaaacgg taaaactatc
     1141 gacggcgcgc aagcggtgct catcaacgtt gtgggtggct ctgattttgc actgtatgaa
     1201 taccatgaaa ttcaaaaaca aatccacgag caggtttcta aagatgcggt aattattgtc
     1261 ggttttaccg aagacgagac caaagacaac catgtttcaa ttacagtcat tgcaaccggt
     1321 taccgccgta caagcacagc agatacacgt ccgcaagcgc ccacacagca acccccacac
     1381 gcgggacaac ctttccgtcc gcaaatttta ccgcgcacgg gtactgaaga tccaaactac
     1441 cgttcgccta cacagcaggg gttggatttc ccccgtgtcg acgcttccgt ccaagcagca
     1501 gcgtacgata taccaacttt tttgcgtaaa atgccgaaaa tagatggata atgccatggc
     1561 cttatctctt ttttaatttt gtagcattta ctcttcttta tagcggccat gtcgtggcgg
     1621 ggataactac cctcgtcacc gaagccgctt tttttgtgct cgatgcacgg cgggccgggc
     1681 gtaagctcct ctttcctgtg cttttaggtt tacttttagg gttttctctc tttatattac
     1741 ggttaccgta cctttcatta actaaccaca actttgaatc cggcgaagcg gtggtgactg
     1801 cagtctcgcg taaatcggtt gttatcgcgc gtgtagataa aataaaacta cgccttattg
     1861 gcatccccaa agaaaaattg ccggccaact aggcacggat cgtctatacg tgcaaagagc
     1921 aaccggtgcg tgcgtcgact ttccgcacgt tggagcgcat ctctggggtg agagcgtggt
     1981 gtaaagtaac gacgcttgat atccagcatg ttccctcatc cgtttggagt cgctggcgtg
     2041 cggctacctt ccattttttg aacgcgcggt ttgacacaat catgcaaaaa aataaaagcc
     2101 ttgtcgccgc tttcctgttg ggtgacactg aagatttaag cgcaagcgaa ttagaggcat
     2161 tccgttcgat gggattgatg cacctctttg cggtttcggg actcaatatc gctttgctct
     2221 ttgcgctact ctatatccct tttcggttaa ttggccaaaa agcaattggg actgcgctgg
     2281 gttataccgt cgccgtatta tttcttttgc tgttggattt tcctgtgccg cttttgcgtg
     2341 cgtgggtatt tatgacgatt gcacttttca tgcgcgtttt ggatcggcgt ataccgtcgt
     2401 ggacatcgct ttttctcacg gcaatttttg ttgaaattct ttttcctctc tcgacatttt
     2461 caatgtcgtt tattctttcg tttggggtga ccgctgcaat ttttattttt tatgaaccgt
     2521 tctatttttg ttttgcttcg tcgaaaaaat acttaaagct gtggaacgaa catcttgcgc
     2581 taacgctcgc tgccggcttg ccagcactcc tcctcactta ttttttgttc ggtcaagcaa
     2641 atccattgtc gcttctctat aatttgttat tggtgccttt ttcaggcatc tatcttttcg
     2701 cggcactcat ctttctcttt tttgatcccg cacaatatat ttttttgccg ttagacactc
     2761 tctatctaga atttgccgag tggcaccaaa cgtacatcag cgcgaacttt ccattagccg
     2821 ataaaacagc gcagcaaatt tcgttggctt ttgtcgccac actcctttta cttctttata
     2881 ttaccaaaat aaggcggcgt ctgtggagcg cgaagcgctt cgttagagtg gtaatcccac
     2941 tgttggtggt tattctattg ctgccgtatt ttttagtcga gtaccccaag aacgcgttct
     3001 acgctatccc taacaaagtg tggatttatt ccgatgcaca gcttaccaca gtgggtgcat
     3061 ctgttttctc tccaccaccc aaagaaccca aaatttgttt ccctgtgcac caccgacacg
     3121 agatagcgct cgataagagg atgccgaaag aaattttgca gcacggtaat gcgtgttata
     3181 ttttttcggg gcgcatgcaa cctgaaaagt ggcgatctca cattctcaag ccatgctcgc
     3241 agctttatct tttacacgga aaagatgtaa cacccaatgt ggctgagtgg aagtctcttt
     3301 ttgtgctctt tggttattcg ggggcggtgg cgctacgtaa ttactttaag tggtacggtg
     3361 atggtattgt cgattgtatg tagtgcctgt ttattcggat agccatctca ctcatggatt
     3421 atgtcacatt tgggggtaac tacgccagtt tttcggatta tgtcacatta ggggataact
     3481 atacctcgcg agtgctgaag tatttaaaag gcaggtaaaa tttgaataaa cactcgcttt
     3541 caggtgggca ggcaactgat ttttctctcc agcaagttgg cgcacaggct ctaaatccat
     3601 tccctctttc gctttgcacc gcgaagcaaa gtgaaagaag taccctcttt tatggatttc
     3661 attgacctca aaacacaaca agcgcgtatc aaaacacaaa tcgatgcgaa catacaaaaa
     3721 gttctcgcac acgggcagta catcatgggg cccgaaatta cagaaataga aaagcggctc
     3781 gctacataca ccggtgccaa acattgcctt accgtcgcca atggtaccga cgcacttctt
     3841 attgcactca tggcgttggg agtaaaagca ggcgacgagg tgatcacttc acctttcacg
     3901 tttatcgcca ccggtgaaat gattgcgctt ttgggtgcgg cgcctgtttt tgtcgacatc
     3961 gacccaaaga catacaatat tgatcccaca aaaattgaag ccgcgatcac tcctaaaaca
     4021 aaagcgatta tgcctgtgag tctctatggc caatgcgccg actttgacgc catcaacgtc
     4081 attgcacaaa agcacaatct ccctgtcatt gaagatgcag cgcagagctt tggcgcggaa
     4141 tacaaaaaaa agaaatcgtg caatttgagt acgattggct gcaccagttt tttcccctcg
     4201 aagccgttgg gttgttatgg cgacggtggc gctgttttta ccaacgacga cgaacttgcc
     4261 aaaaagatgg ggtatatccg cctgcacggg caagataaac gctaccatca tccgatcatc
     4321 ggtgtcaatg gccgtatgga cacgctacaa gcggcgattc ttttacccaa gctggatatc
     4381 ttccctgaag aagttacgct gcgcgaaaaa attggcgcac gttattcaag ccgtcttgcc
     4441 gcgcatggca tcaccacgcc atatatcgag ccctataact tgagcgtata cgcacagtac
     4501 acggttcttg tggataaccg cgaaaccgtg caaaaaaaat tacaagagca tggcatcccc
     4561 actgcggtgc attatcccat tccccttaat ttacagccgg tctttgcgca tctcaatcaa
     4621 ccgaaaggca gcttcccgat tgccgagcgc attgctgagc gcgtggtgag tttgccgatg
     4681 catccttatc ttgatgaggc aacacaagat aaaattatta cagcagttgc cgcagctgta
     4741 tagatcatgc aaactctact tgttgtctta ctttcactcc ttgcgcttct ctcaggcgcc
     4801 gcgttacatt attttttttc gctgcgtcgc gcgttgaaaa gtgtacgcta tgaaatgcgc
     4861 gagctgcgcc agttgcaaag ctcgccgtat aacgacgctg tgttcgatga acccattatc
     4921 cgtatgctcg tcgaagtaaa agatcccatc cgtttggcgc accgcaaatc gcctcttgca
     4981 aaaatcgctt caccaacagc tcccaatctt gtcgctaaaa aagtgtacga acaagtgatg
     5041 caagagacac gcgagcaact taaaggtcgc gaggtcgaag caaacgtgag cctcatcgtt
     5101 ctataaatga ctattgaact cttggttttt tttgcgttag tgggattgat cctgtgtacg
     5161 cttttttttc ttttcttacg aaaaaaagaa gcacaggtgc gccgtttccg ccgtgaaatt
     5221 tttttaaaaa aaactgcact caaaggcgct gcgtttgcgc agttactcaa agcggaaaat
     5281 acacaactca tcgcgcacgg tatcgaccag agcgctctct ttgtgcgcgg tggccatcgt
     5341 ttggtgagcg atgtcacgtt tggcctcatg cgacttatcc ccgcaacgcg tgagcaagca
     5401 cgtgcagcta aagacgtgca cgacttcatt agcgacagtc tctatggagc gttgcgtatt
     5461 atcaacgagc aaatcggcgg cagcatcgca caaaacatca aaatggtaaa accaaaagtc
     5521 aaaacgaaga caacaaaaat taaaaaaaat tccaaaaata ac
//
LOCUS       DAOMPD010000137         5556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6174_length_5556_cov_2.641883, whole genome shotgun sequence.
ACCESSION   DAOMPD010000137 DAOMPD010000000
VERSION     DAOMPD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_5556_cov_2.641883"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1599)
                     /locus_tag="PLY93_06030"
     CDS             complement(28..1599)
                     /locus_tag="PLY93_06030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69071.1"
                     /translation="MVVFSTLREKYLNSGSRHFTQTTDLKRNRMLNMLFSMGIVASFY
                     IAVIEVVFAAILITRDYDRYISYLFPFVSVIVIYPILVAITFFIKNITGSIHVTLLNN
                     FLLNGFCLTFALFLGEKAQMHFIILSHIPIIFLIYRFGSWKSITAHLLLAAVGLIAIL
                     GVYRFTQPLYPLPDDLAYIPGYLCWIATFGVLVWYSIYNWKLVFVTESLLEEERDQTQ
                     KLLNETIPKLEKAETKYRHLVDGSNDLIFLLSTDLTILNMNKSSQSMLAFLPTDMECK
                     NLTVFIAERAGHNTMMSHNIVREQAAQALMSNKPVRFRTRFIHKHREDGVEVLLSFQA
                     SRFNQEVEIMARAVEVEPEIMLRFLERENGNYTLDNNILHADILSERIADRISSYYNA
                     FTLNLLRTCIREILINAIEHGNLDITFEEKSRVIESQNFIEFLRLRQKEPQYMNRKVR
                     VRYAITREKFIIRITDEGKGFDYVSFLKRTVEDDSMLMLEHGRGITMTQNTFDEVRFN
                     DKGNQVTLIKYHRVL"
     gene            2142..4211
                     /locus_tag="PLY93_06035"
     CDS             2142..4211
                     /locus_tag="PLY93_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRP69072.1"
                     /translation="MQQKCVCHGEGPLLVLAGAGSGKTGVIVNRITHLIEAGTKASAI
                     CAVTFTNKAAREMRERVREIVGKGAQKTMPLRAPHGKNSALSIKGIFIGTFHSLGLTI
                     LRENLEAAGIRENFLITGRDDQLTLAREILVHSGVDVERYDEKQLIKSISDWKNSGEA
                     KNAFFESDLWKLKIPERPILEIVDEYLTRLKEMNALDFDDLILLPTRLLENNQPVREK
                     YRARFSYFLADEFQDTNPLQFHFLTQLMRAPMNLTAVGDDDQSIYSWRGADTRIMLEF
                     KTRFPESEIVALEQNYRSEQYILDIANRLIQNNTHRHAKNLFSDRKIPNQAKVFEAAD
                     GLAEAEYAADTIVTLKVKHNLEYSAFAVLYRTNFQSRSFEEILRQRNIPYHVSGSFQF
                     YDRSEIKDILAYLRFFANHADDRSLMRILLMPKRGISEAAIGRLTQYAHLNKKHLWDI
                     LTEIEMCEMEVSSAALTGILEFRDFVAEHETEIRKPGQLAKAAQKLVDALQLEKEYVR
                     QGVEAQKIKNKLLNVRELIRSMEDFETGDRQILGVTEGVERWLYTYLQFVALITADDK
                     ESANDLPRVELMTIHQAKGLEFDTVFVTGLEEGVLPHQRSVDAAEENEDTAIEEERRL
                     MYVAITRAKKRLYLTYSASRKNRGMTLECSPSRFLDELPQHLLEWENLTNAELDTANL
                     ETLFSAS"
     gene            complement(4185..4679)
                     /locus_tag="PLY93_06040"
     CDS             complement(4185..4679)
                     /locus_tag="PLY93_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69073.1"
                     /translation="MRIFFPLFLLTLALSVASARQKKTVTKKIVEPKTWEEKKRIAKE
                     MKKISKQLDVKCEYCHADAERGLKEGDYTLLTEEGEYAHETMFPLSKTFKVDCAFCHT
                     GTSTMTPAGERAHQDMKFIKKYKRERKKNLTCESCHTPGVKGREFSTLKKTAQQLRGR
                     EERF"
     gene            complement(4690..4950)
                     /locus_tag="PLY93_06045"
     CDS             complement(4690..4950)
                     /locus_tag="PLY93_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69074.1"
                     /translation="MLNEEAVDAPSREEMLRRALEEQLARQTSAEHALPIERLMDRLS
                     AGKKLKDEEYAAISKEIGDIQLRNHEHLARLEDLLSAFHHSK"
     gene            complement(5008..>5556)
                     /locus_tag="PLY93_06050"
     CDS             complement(5008..>5556)
                     /locus_tag="PLY93_06050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRP69075.1"
                     /translation="DGHALFNRYSLFEKGDAVVRDAKESLTIFSTPAIEGGMRSYAFD
                     DLGFPIQEVCLIRDAHVENFLIDGRYADLLKMPATSALANVRVAHGSTPYADFLSDGV
                     LELSKFSTFQPNTVSGAFSGEIRLGYWHKNGRKIPIKGGSVSGSTQSAFARAYKSREE
                     VKRASYLGPKGVFFENLTVAGS"
BASE COUNT         1512 a         1319 c         1266 g         1459 t
ORIGIN      
        1 cgtaaacaga ggaaagtaca aagcttttta aagaactcga tggtacttaa tcaacgtcac
       61 ttggttacct ttatcgttga agcgcacttc atcgaaggta ttttgagtca tcgtgatacc
      121 gcggccgtgt tccaacataa gcatgctatc gtcttcgacg gtacgcttca aaaagcttac
      181 ataatcaaag cctttgcctt catcggtgat tcggatgata aatttctcgc gggttattgc
      241 ataccttacg cgcactttac ggttcatgta ttgcggttct ttttgtcgca atcgtaggaa
      301 ttcaataaaa ttctgagatt caatcacgcg agatttttcc tcgaacgtta tatctaagtt
      361 gccgtgttca atcgcattga tgaggatttc gcggatacaa gtgcgtaaaa gattgagggt
      421 aaaagcattg taatacgaag atattctatc ggcaatgcgc tcgctcaaaa tgtctgcatg
      481 caaaatattg ttatcgagag tatagttccc gttttcgcgc tctaagaagc gcagcataat
      541 ctcaggctcg acttcgacag cgcgcgccat aatctccact tcttggttaa aacgacttgc
      601 ttgaaagcta agcaaaacct cgacaccgtc ttcgcggtgc ttatgtataa accgagtgcg
      661 aaagcgtacc ggtttgttgg acatgagtgc ctgcgccgct tgttcacgca caatgttgtg
      721 gctcatcatg gtattatggc ctgctctttc cgcaataaat acagttaagt ttttacactc
      781 catatctgtc ggtaagaatg ccaacatact ttgcgaactc ttattcatgt tgaggatggt
      841 gaggtcggta cttaaaagaa aaattaaatc gtttgaacca tcaacgaggt ggcggtattt
      901 tgtttccgct ttttctaatt tggggattgt ttcgttgagc aacttttggg tttgatcgcg
      961 ctcttcctcg agcaaggatt cggtgacaaa gacgagtttc caattatata tcgagtacca
     1021 aactagcacg ccaaaagtgg caatccaaca aaggtaaccg gggatataag ctaaatcgtc
     1081 gggtaatggg tataacggtt gagtaaaccg ataaacccct agtatggcga taagacccac
     1141 ggcggcgaga agtaaatgtg cggtaatcga cttccaactt ccaaaacgat agatgagaaa
     1201 aataataggg atatgagaaa gtataataaa atgcatctgc gctttttcac ccagaaatag
     1261 cgcgaaagtt aaacagaagc cgttgagaag aaaattattg agtaacgtta cgtgtatcga
     1321 accggtgata ttcttgatga agaatgtaat cgctacaagg ataggataaa ttacgatgac
     1381 gctgacaaaa ggaaataggt atgatatata gcggtcataa tcgcgggtta ttaaaatagc
     1441 cgcaaaaacg acttcaataa ctgcaatata aaaactggcg acaatcccca ttgaaaaaag
     1501 catattgagc atgcgattgc gcttaaggtc ggtcgtctgt gtaaaatgac gactacccga
     1561 attgagatat ttttcgcgga gtgtagagaa aaccaccacg gtaaagtctg agaggtgtat
     1621 gtgtgatgta aagattgaat tagcttatag acaaagttta ctgaacatct attagtggtc
     1681 tacgtacact ctcaggggtt ggcgcacagg cctccaagca accacgtgta acattagcac
     1741 agcatgttac aatttggaat gcgcaagctg ttaaggtagc tattcctatt tttaagatta
     1801 tgtcacatta aggggtaact ataccaatct ttaagattat gtcgcattaa ggggtaacta
     1861 tgccaatttt tcagattatg tcacattaag gggtaactat gccccacgag tactggaatg
     1921 gtcaaaatca gagaaaacct taagaaacac tttcgtttat ataggcaagc tatcaatata
     1981 gctggcagcg cttagttgta tgcttacttg gctgtgctcc aactcgctga gataattcct
     2041 ttgctgattt ttccacacgc acaggctcgc acccaccttt ccactggtcg tattatgtcg
     2101 cattgtaaat ttgacttact gtgccctacc ccttaaacgc catgcaacaa aaatgtgtgt
     2161 gccatggtga aggcccgctt ttggttttgg cgggtgcagg ctcaggcaaa accggcgtta
     2221 tcgtcaaccg catcactcat ctcattgaag caggcacgaa agcaagcgcg atttgtgcgg
     2281 taacttttac taacaaagcc gcgcgcgaga tgcgcgagcg cgtgcgcgag atcgtgggta
     2341 aaggcgcaca aaaaaccatg ccgcttcgag ccccccacgg gaaaaattcc gcgctgtcca
     2401 tcaaaggtat tttcatcggc acattccaca gcttggggct cacgatactg cgtgaaaatt
     2461 tagaggcggc gggtattcga gaaaattttc tcatcacagg ccgcgacgat caactcactc
     2521 tcgctcgtga aattctggtg cattcggggg tggatgtaga gcgttacgac gaaaaacaac
     2581 tgattaaatc aatttcggac tggaaaaatt cgggcgaagc caagaatgct ttttttgaaa
     2641 gcgacctgtg gaaattaaaa attccagagc gccccattct tgaaattgtc gacgagtatt
     2701 tgacgcgcct taaagaaatg aatgcactcg actttgacga tctcatctta ctaccaacgc
     2761 ggcttctcga gaataaccaa ccggtacgcg aaaaatatcg agcgcgcttt tcttattttt
     2821 tggctgacga gtttcaagac accaatcctt tgcagtttca ttttttaacc caactcatgc
     2881 gcgcgcccat gaacctcacc gcagtgggcg acgacgacca atcaatttat tcgtggcgtg
     2941 gcgccgacac gcgcatcatg ctcgagttca aaacgcgatt ccccgaatct gaaattgtag
     3001 ctctcgaaca aaactaccgc tccgagcaat acattctcga catcgccaac cgcctcatcc
     3061 agaacaacac gcaccgccac gcgaaaaatc tgttcagcga ccgcaaaatc cccaaccaag
     3121 ccaaagtttt tgaagcagcc gatggacttg ccgaggccga atacgccgcc gacaccatag
     3181 ttactctcaa ggttaaacat aacctcgagt actcggcgtt tgccgtgctt taccgcacta
     3241 actttcaaag ccgttcgttt gaggaaatct tgcgccaacg gaatatcccc taccacgtca
     3301 gcggtagctt tcaattttat gaccgcagcg agatcaaaga tattttagct tacttgcgtt
     3361 tttttgccaa ccacgccgac gatcgttcgc tcatgcgtat tttgctcatg cccaaacgcg
     3421 gtatctcgga agcggcgatt ggccgtctta ctcaatacgc gcacctcaat aaaaaacatt
     3481 tgtgggatat actcacagaa attgaaatgt gcgaaatgga agttagctcc gctgcactca
     3541 ccggcatttt agagttccgc gactttgtcg ccgaacacga aactgaaatc cgtaaacccg
     3601 ggcagctcgc caaagcggca caaaaactcg tcgatgcttt gcaactcgaa aaagaatatg
     3661 tacgacaagg cgttgaagca caaaaaatta agaacaaact tttaaatgta cgcgagctca
     3721 tccgttcgat ggaagacttt gaaacaggcg acagacaaat cctcggggtg accgaaggtg
     3781 tggaacgctg gctctatacc tatttgcaat tcgtcgcgct catcaccgcc gacgataaag
     3841 aaagcgcaaa cgatttgccg cgtgtggaac tgatgacgat acaccaagcg aagggattgg
     3901 aattcgacac cgtttttgtc actgggttag aagaaggcgt attgccgcac caacgtagcg
     3961 tcgacgccgc cgaagagaac gaagacaccg ccatcgaaga ggaacggcgg ctaatgtacg
     4021 tggcgataac ccgcgctaaa aagcgccttt acctcaccta ttcggcgagc cgtaaaaacc
     4081 gggggatgac tttggaatgc tcgccttcgc gctttttgga tgaactgcca cagcacttgc
     4141 tcgaatggga gaatttaaca aacgccgagc tcgacaccgc caatttagaa acgctcttct
     4201 ctgcctctta attgttgtgc ggtctttttg agcgttgaaa actcgcggcc ttttacgcca
     4261 ggggtgtgac aactttcgca agtgagattt tttttccttt cgcgcttgta ctttttgatg
     4321 aacttcatat cttggtgcgc gcgctcaccc gctggcgtca ttgtggaagt gccggtgtgg
     4381 caaaacgcgc aatcgacttt gaaagttttg cttaaaggga acatggtttc gtgcgcgtac
     4441 tcgccttcct cggtgagcag agtgtagtcg ccttctttaa gcccgcgttc ggcgtcggcg
     4501 tggcaatatt cgcatttcac atcgagctgt tttgaaattt ttttcatttc cttcgcgata
     4561 cgttttttct cttcccatgt tttaggttct acgatttttt tcgtcacagt ctttttttgc
     4621 cgtgccgatg cgacgctcaa agccagcgtt aataaaaata aagggaaaaa gatacgcatg
     4681 gaaaaaatcc tattttgagt gatggaacgc cgagaggaga tcctcaaggc gcgctaaatg
     4741 ctcgtggttg cgtaattgaa tatcgccaat ttccttcgag atagcggcgt attcttcgtc
     4801 tttgagtttt tttcccgcgc tcaacctgtc catcaaacgt tcgatgggga gtgcgtgttc
     4861 ggcgcttgtt tgacgcgcca gttgctcttc taacgcacga cgcagcattt cttcgcgcga
     4921 cggcgcatct acagcctctt cattcaacat cgcctttgtg cctcaattcg tttatcgctc
     4981 tgtcaaccga aattgcccct taaatgatca acttcccgcc acggtcaaat tctcgaagaa
     5041 aacaccctta ggtcccaaat acgatgcgcg ctttacttct tctctagatt tatacgcacg
     5101 tgcaaacgca ctttgtgtac tgcccgaaac cgagcctcct ttaattggga ttttcctacc
     5161 gttcttatgc caatagccca aacggatttc ccctgaaaag gcgcccgaga cggtgttggg
     5221 ttgaaacgtc gaaaatttgc tgagttctaa aacaccatcg cttaaaaaat cggcataagg
     5281 agtactaccg tgcgccacac gtacgttcgc aagcgcgctc gtcgcaggca ttttcagcaa
     5341 gtcggcgtag cggccatcga tgaggaaatt ttcgacatgc gcatcgcgga tcaaacagac
     5401 ttcttggata ggaaatccca aatcgtcgaa tgcatacgag cgcatccccc cttcgatggc
     5461 gggtgttgag aaaatagtga gcgactcttt cgcgtcgcgc accaccgcgt cgcctttttc
     5521 aaaaagcgaa tagcggttaa agagcgcgtg gccatc
//
LOCUS       DAOMPD010000138         5545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6186_length_5545_cov_2.857741, whole genome shotgun sequence.
ACCESSION   DAOMPD010000138 DAOMPD010000000
VERSION     DAOMPD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6186_length_5545_cov_2.857741"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLY93_06055"
     CDS             <1..186
                     /locus_tag="PLY93_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRP69076.1"
                     /translation="QDVRTHFKKQVYDVIIPRNVKLSEAPSFGQPINVYDPASTGAIA
                     YKTLAKDLLARAKEAHE"
     gene            179..1060
                     /locus_tag="PLY93_06060"
     CDS             179..1060
                     /locus_tag="PLY93_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRP69077.1"
                     /translation="MSKGAGKVLGRGLSNLLDHTTLPEAGERVQEIDIGLIKPNPENP
                     RKKFDRTAIDELAQTIKEHGLLQPILVQKKGKAYIVISGERRLRSLLSLGYSKAPCIV
                     KEFDRQKTLEVSLIENIQREQLDAIEEATVYKTLMTDFSLTQEDIAERVGKNRTTVAN
                     RLRLLHLPDVVQAALADGRLTEGQARPLLSIKNSELQLKLFKEITTGQGLNAREIEER
                     ARKLAHPKEEKKNQSHKKTDANIKALAQKLEEHLDMRTRILYNAQKKNGSVKVDSFSL
                     DDLEKLLKILGLKRFTL"
     gene            1064..1387
                     /locus_tag="PLY93_06065"
     CDS             1064..1387
                     /locus_tag="PLY93_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69078.1"
                     /translation="MKEQNFLNTVTQRASHYTPGEQVNEEGWIKLNTNESPLPASPQV
                     FATLKALASQVDVLRKYSHPLGGTLGYRQILSRGDERFRRSADASLPRNIGRRAQRCL
                     SRSDV"
     gene            1293..2135
                     /locus_tag="PLY93_06070"
     CDS             1293..2135
                     /locus_tag="PLY93_06070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRP69079.1"
                     /translation="MVTNGSDEALTLLCRATLDAERSAAFPEVTYSLYKTLVTNAGAS
                     YTEVPVIPSDNQPYGVDVAALENSAAQVVFLANPNAITGEYSPREKLKATLAQSHKLW
                     IIDEAYNDFAGHDATMLPFVPDLKNLVVTRTFSKTHALAGLRVGYLATSNHALLNGLM
                     AHKDSYNEDALATRLAKVALNDTAWHQKTLDAVREGHAYLFNTLASMGFTPYTSSANF
                     LLVKVPPPVSAEALAQKLRDYKLLIRYYPNSKFSDCVRISVGAMEENQKLIAAIGSIY
                     AHAQ"
     gene            2247..3182
                     /locus_tag="PLY93_06075"
     CDS             2247..3182
                     /locus_tag="PLY93_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRP69080.1"
                     /translation="MKGHERFRSKDKEDGIPLFGTRPAWIHLYPKNEKDLVSLLHRYN
                     IHPLTVEDVFNSSNRIKREKFEHYTYFCFRGVHLTGGYISTKDFNFILTKNTLITISE
                     SGRNTIDSLLENKKLCHELLKKGPEFIFHRILDIETDHTLHIVRDIDTAFEKNEHLLL
                     TFNAKLDIATVFELKSSLALIKKLILAHKEIFDEIHIYDMPNFSPDSAAFFRDVRDHA
                     IKVIDIIDGIAQAITSAVEAYNTISTKRTNDIIRILTILTAIMLPLTLVTGYFGMNFK
                     NLPLTEDHYGYLKTILAMGVLAITMVIFFLKKKWL"
     gene            complement(3179..3577)
                     /locus_tag="PLY93_06080"
     CDS             complement(3179..3577)
                     /locus_tag="PLY93_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69081.1"
                     /translation="MKFFYFISLFLVLGVKPKTVWVVHEYIGGAQCVTMGKEAAFTAP
                     GFIEASEPFIKNRIAILRSYFRDLPVCQACHTCARYRRQIWFEIEEKYLQKASQVGFT
                     PSNENPTDDELAEYKRSKKFEPKVPMPQGD"
     gene            3605..4204
                     /locus_tag="PLY93_06085"
     CDS             3605..4204
                     /locus_tag="PLY93_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP69082.1"
                     /translation="MKQEKRKELTREKILTAGKQVFAQKGYAGTSIADIVKKSKLARG
                     TFYLHFQSVEQVFNALLQEVFIDIQRNLTELQVEILDQKNFKKDLLVLAKSLLVVFQE
                     RKEIVGILLTTMNSDAKVRTQTIWFQELLQASIRVMFDRGIQSGRLRKFDSELMSYLV
                     AGGLREVLAQWILYGRYADRIEKKVNEVVDIYMNGVEKN"
     gene            complement(4201..4704)
                     /locus_tag="PLY93_06090"
     CDS             complement(4201..4704)
                     /locus_tag="PLY93_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRP69083.1"
                     /translation="MLKTLTTEELKVLNALGENGLLTNAELATLLSLDEAKAGEIRSN
                     LEASGIVLKYRAIINWEKIESSSVNALIQVKVTPAHGHGYDEVARKVAAFPEVRTCLL
                     VSGSFDLLVEVDGPSLRDVAFFVADKLATIEGVNHTSTNFLLKRYKQSGDMFSVSGKT
                     HRLPMMI"
     gene            4782..5066
                     /locus_tag="PLY93_06095"
     CDS             4782..5066
                     /locus_tag="PLY93_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69084.1"
                     /translation="MREKVYCANCEHCHVVRVFETDASKYVLRVKCAKKRWAKRSGEE
                     KQYKYFTVARRVVSDCPDYSPMGDPNPFIKNLRRELPVKDEIYTLKEGEA"
     gene            5123..>5545
                     /locus_tag="PLY93_06100"
     CDS             5123..>5545
                     /locus_tag="PLY93_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP69085.1"
                     /translation="MKKPFKILGVQQIAVGGADKTRLRQLWVDTLGLTTKGSFRSEKE
                     NVDEDILELGQGAHAIEIDIMQPIDPTKKPKVDDPPLNHIGLWVDNLAACVEYLTQQG
                     MRFTPGGIRKGAAGHDVCFIHPKGNDDLPLSGEGVLIEL"
BASE COUNT         1593 a         1384 c         1257 g         1311 t
ORIGIN      
        1 caagacgtgc gcacgcattt taagaagcaa gtgtacgatg tgattatacc gcgcaatgta
       61 aaactttccg aagcgccgtc gtttggccaa ccgattaacg tgtatgatcc cgcatcgacc
      121 ggcgcgattg cgtacaaaac actcgcaaaa gatctgcttg cgcgcgcaaa agaggcgcat
      181 gagtaaaggc gcaggcaaag tcttggggcg cggactttcc aatctactcg accacaccac
      241 gctccccgaa gcgggcgaac gggtgcaaga gattgacatt gggcttatta aacccaaccc
      301 tgaaaacccg cgcaaaaaat tcgaccgcac tgccatcgac gaattagcgc agaccatcaa
      361 agagcatggt ctattgcaac cgattctggt gcagaagaag ggcaaagcgt atattgttat
      421 ttcaggagag cgccgtttgc gttcgctctt gtcgctggga tactcgaaag cgccgtgcat
      481 cgtcaaagaa ttcgaccgcc aaaaaactct cgaagtctct ctcattgaaa atatccaacg
      541 cgagcaactc gatgccatcg aagaagcgac ggtctataaa actctcatga cagatttctc
      601 tctcacgcaa gaagatattg ccgagcgtgt cggcaaaaac cggacgactg tggccaaccg
      661 cttgcgtctt ttacatttgc ccgacgtggt gcaagccgcg ctcgccgacg gcaggctcac
      721 tgaaggtcaa gcgcgtcccc tcctttcgat taaaaatagc gaactgcaac tcaagctctt
      781 caaagagatc accacagggc aaggactcaa cgcgcgcgaa attgaagaga gggcgcgcaa
      841 actcgcgcat cctaaagaag aaaaaaagaa ccaatcccac aaaaaaaccg atgcaaacat
      901 caaagccctc gctcaaaaac tcgaagaaca cttagatatg cgcacacgca ttctatataa
      961 cgcacagaaa aaaaatggta gcgttaaggt cgattctttt tcactcgacg atttagaaaa
     1021 attgttgaaa attttaggac tcaagcgttt tactttatag gttatgaaag aacaaaattt
     1081 cttaaacacg gttactcaac gcgcatcgca ctacacgccg ggcgaacaag tcaacgaaga
     1141 ggggtggata aaactcaaca cgaacgaaag tccgcttcct gcttcgccgc aagtttttgc
     1201 cacgcttaag gcactggcga gccaggtcga cgtgttgcgc aagtatagcc acccgctggg
     1261 cggcacactg gggtatcgac aaatcctctc tcgtggtgac gaacggttcc gacgaagcgc
     1321 tgacgcttct ttgccgcgca acattggacg ccgagcgcag cgctgccttt cccgaagtga
     1381 cgtatagttt atacaaaact cttgtcacaa acgcaggggc gtcttacacc gaagttcccg
     1441 tcatcccgag cgacaaccaa ccgtatggcg tcgatgtggc ggcgttggaa aattctgcag
     1501 cgcaggtggt ctttctcgca aaccctaacg caatcacggg tgaatacagc ccccgcgaaa
     1561 aattaaaagc gactctggcg caatcgcata aactgtggat tatcgacgaa gcgtacaacg
     1621 actttgccgg acacgacgca acgatgctgc cttttgtgcc cgatttaaaa aatctggtgg
     1681 tgacgcgcac gttttccaaa acacatgcgc tcgccggatt acgcgtgggt tacttggcga
     1741 cctcgaacca tgcgctccta aacggtctca tggcgcataa agattcgtac aacgaagacg
     1801 cgctcgccac gcgtcttgca aaggtcgcgc taaacgacac cgcgtggcat caaaaaacac
     1861 tcgatgcggt acgcgaaggc catgcatatt tgttcaacac tcttgcctca atgggattta
     1921 ccccgtacac gtcgtcggca aattttttat tagtcaaagt cccgccgccg gtgagcgcag
     1981 aagcactcgc gcaaaaattg cgcgattaca aattgctcat tcgctactat cctaactcta
     2041 aattttcaga ctgcgtacgc attagcgtag gcgcgatgga agaaaatcaa aaactcattg
     2101 ccgctatcgg cagtatttat gcacacgcac aatagtaagc atggattatg gcgtaccggt
     2161 ttttatcatg gttgactttt aaaaaaagac cggcctcaat agcgacgcat gtaaaaaaag
     2221 actggtacga aaatcacatc gtaaccttga aaggccacga gcgtttccgt agcaaagata
     2281 aagaagacgg gattcctctt tttggcacac ggcctgcgtg gatacactta tacccgaaaa
     2341 acgaaaaaga tttagtctct ttattgcatc gatacaatat ccaccctttg acggtcgaag
     2401 acgtgttcaa ctcgtcaaac cgcatcaaac gtgaaaagtt cgagcactac acctatttct
     2461 gttttcgtgg agtccatttg accggtggtt atatttccac caaagacttt aactttattt
     2521 taactaaaaa cacgctcatc accatcagcg agagcgggcg caatacgatt gattcgttgt
     2581 tggagaataa aaaactctgc cacgaactat tgaaaaaagg gccagagttt atcttccacc
     2641 gtattttaga tattgagacc gaccacacat tgcatatcgt acgcgacatc gatacggcgt
     2701 ttgaaaagaa cgaacatcta ctgttgacgt ttaatgcaaa actcgacatc gccactgtgt
     2761 ttgaactcaa gtcgtcgttg gcgctcatca aaaaactcat tttagcgcat aaagaaattt
     2821 ttgatgaaat tcatatatac gacatgccaa atttctctcc cgattcggcg gcgttttttc
     2881 gcgacgtgcg cgaccacgcc atcaaagtca tcgatattat cgacggtatc gcacaggcga
     2941 ttacctctgc ggtcgaggcg tacaacacta tttcaacaaa acgcaccaac gacatcatcc
     3001 gcatcctcac cattttgacc gcaattatgc tacccctcac tctggtgaca ggttactttg
     3061 gtatgaactt taaaaattta ccgctcaccg aggatcatta cggctatttg aaaacgattc
     3121 ttgcgatggg ggttttggca attacgatgg ttattttttt tctcaaaaaa aaatggcttt
     3181 agtcgccttg cggcattgga actttaggtt caaatttttt tgatcgtttg tattctgcca
     3241 actcatcgtc ggtgggattt tcattactcg gcgtaaatcc aacttggctg gctttctgta
     3301 ggtacttctc ctcaatttca aaccaaattt gccgacggta cctcgcgcaa gtgtggcacg
     3361 cttggcacac aggcaaatca cggaaataag aacgcagaat agcgatacgg ttttttataa
     3421 acggttcact tgcttcgata aatccgggcg cagtgaacgc cgcttcttta cccatagtta
     3481 cgcactgcgc accgccgata tattcatgta caacccaaac agttttaggc tttaccccca
     3541 agactaaaaa aagcgatatg aaataaaaaa acttcatgga ctattttatt atcggcaaat
     3601 ttgcatgaaa caagaaaaac gaaaagaact cacccgcgaa aaaattttaa ctgctggaaa
     3661 acaagtcttc gcgcaaaaag ggtatgcggg cacttcgatt gccgacattg taaaaaaaag
     3721 caaactcgca cgcggtactt tctatctaca ctttcaaagc gtggagcaag tttttaatgc
     3781 gcttttgcaa gaagtgttta ttgacatcca acgcaacctc accgagttgc aagtggaaat
     3841 tttagatcaa aagaacttca aaaaagatct tttggttctt gcaaaatcgt tgctcgttgt
     3901 gtttcaagag cgcaaagaaa ttgtgggtat tcttttaacc accatgaact ccgacgcaaa
     3961 agtccgtaca caaacaattt ggttccaaga acttttgcag gcgtcgattc gggtgatgtt
     4021 cgaccgtggc atccagtcgg ggcgtttgag gaagttcgac agcgagctca tgagctacct
     4081 tgtcgccggc ggattgcgtg aagtcttagc tcaatggatt ttgtacggca gatacgccga
     4141 ccgtatagag aagaaagtca atgaggtagt ggacatatac atgaacgggg tagaaaagaa
     4201 ttaaatcatc atcgggagtc ggtgcgtttt gccactcacc gaaaacatat cccccgactg
     4261 cttgtaacgc ttcagcaaaa aattggtgct ggtgtggttg acgccttcaa tcgtcgcaag
     4321 tttgtccgcc acaaaaaaag ccacgtcgcg caacgaggga ccatccacct cgacgagtaa
     4381 atcaaacgag cccgaaacta aaagacaagt gcgcacctcg gggaaagcag ccaccttgcg
     4441 cgccacttcg tcgtaaccat gcccgtgcgc cggtgtgact ttcacttgaa tgagcgcatt
     4501 gacgctggag ctttcaattt tctcccagtt gataattgcg cggtatttaa gtacaatacc
     4561 cgatgcttcg aggttgctcc gaatttcgcc cgcttttgct tcgtcgagac ttaggagcgt
     4621 cgcaagttca gcgtttgtca aaagcccgtt ttcacccaat gcgttgagca ctttgagttc
     4681 ttcggtggtg agcgtcttta gcatgcgtga agacaaatcg ttgaaacgac cggtcaattc
     4741 tttcttgccc cgatgtctca cccctctgaa acgaccatag aatgcgagaa aaagtctatt
     4801 gtgccaactg tgaacattgc catgtcgtgc gcgtctttga aaccgacgcg tccaagtatg
     4861 tgttgcgcgt aaagtgcgcc aaaaaacgct gggcgaaacg ctcgggtgaa gaaaaacagt
     4921 ataagtattt tacagtcgcg cgccgcgtcg tgtccgactg ccccgactac tcgccgatgg
     4981 gcgatcccaa cccatttatc aaaaacttgc gccgcgaatt gccggttaaa gacgaaattt
     5041 atactcttaa agaaggcgaa gcgtagttcc atttttatct gtgagatgta tgcatctcac
     5101 agataaaaaa ataaatccca ctatgaaaaa accattcaaa atactgggcg tacaacaaat
     5161 tgccgtgggt ggtgccgaca aaacacgtct acgccaactg tgggttgata cgctggggct
     5221 aacgactaaa ggctctttta gaagcgaaaa agaaaacgtc gatgaggaca tccttgaact
     5281 aggacaaggt gcgcatgcga tcgaaatcga catcatgcaa cctatcgacc caacaaaaaa
     5341 acccaaggtg gacgatcccc ctctcaacca tatcgggctg tgggtggaca acctcgccgc
     5401 ttgtgtcgaa tatctaaccc aacaaggaat gcggttcaca ccagggggaa tccgcaaagg
     5461 cgcagcgggt catgatgtgt gcttcatcca ccccaaaggc aacgatgacc tcccgctgtc
     5521 gggcgaaggg gtattgattg agctc
//
LOCUS       DAOMPD010000139         5526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6218_length_5526_cov_3.065436, whole genome shotgun sequence.
ACCESSION   DAOMPD010000139 DAOMPD010000000
VERSION     DAOMPD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_5526_cov_3.065436"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..677)
                     /locus_tag="PLY93_06105"
     CDS             complement(15..677)
                     /locus_tag="PLY93_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP69086.1"
                     /translation="MSKELHSLKINEIFYSIQGEGSYALYPCIFIRLTGCNLRCVWCD
                     TTYSYYEGTTQTFEQILQTIQKYPCDLVELTGGEPLLQKNVYTLFELLHKEKKRVLLE
                     TSGSIPIDKVPPYVHIVLDIKPPDSGEDKKNWYENLQWIKPTDDVKFVVASHSDFLFA
                     EKITEQYALHTKLERPPIVQPVFAQLSGSEVGEWVKNSCFSFRLGLQLHKFLYEPMLR
                     AV"
     gene            689..1492
                     /locus_tag="PLY93_06110"
     CDS             689..1492
                     /locus_tag="PLY93_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP69087.1"
                     /translation="MRKIKATVNLFLVFPFALFAVEKKSAYLMAERSDTHYEIISSKN
                     TQLIAPMGSLLKPFAAWYLLERGIDSEQVIYCPPRRKQTKSLRCWIDTGHGAMTLKTA
                     LAQSCNYYFLSRFLGLNLVEYETWLQNKFDWPITLKITKPENVYGFDLTGGMDVKKIL
                     SMYTKLLNASDEGNPHAETIMDGLSQTCQGTLQDFCSAIAKNRSVRFIAGKTGTIIDA
                     GKNYGIAFLYLEYLPLKQKIILLCYEKNKMGSQAALNAVTILNGYRRKK"
     gene            1470..2411
                     /locus_tag="PLY93_06115"
     CDS             1470..2411
                     /locus_tag="PLY93_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69088.1"
                     /translation="MATVEKNNSEKTSASDWLSFWGSIQNPNRMTLFIGFVVLMFFTV
                     FLGYFGMRTLVRSFDYYRISFEAPFIEDIRPGTKIRYQGALEIGEVVYMKSSASGHRI
                     VAKIRDDFHIPKIGSRASLSSWGYFGAKFINIEVMPTTFNQDFYTLDTPMPLEKIINS
                     TIMFQQYYDAIKSEGGELSVLEKRLSQLKKTVLDIKKTPYVERHRIRSIVRNAASTTT
                     QMYSVVNSTSADLYKMLKQLNDASEYLVYDLNKNLPSIKAKMDKFEQSVTTDGQSFTS
                     RLLHEETIYYLLLDYVKFTNEKLNDMASAPYRIFFNQ"
     gene            2411..2629
                     /locus_tag="PLY93_06120"
     CDS             2411..2629
                     /locus_tag="PLY93_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69089.1"
                     /translation="MAEKIKLKVKCHACSYVMEGTAKYGKGNYHPEGLDFAFTAVGKL
                     VDKAGASRVKGQVEIICPNCETRNRFDI"
     gene            2601..3107
                     /locus_tag="PLY93_06125"
     CDS             2601..3107
                     /locus_tag="PLY93_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69090.1"
                     /translation="MKLAIVLIFSFFSRSLFAECTRAIHFSSVKLAWEKITSAYGDTP
                     LPKEIFICIGRNYFFEIPRGSTMRSVLDRKNKRILLRKTSMHDLTHELLHLYLDLRWQ
                     VLPYSISEPFVVAMNNFNSCPNIHEIKSSLPERWQRRKEIDTCGVKNLIIDILQASTD
                     ERKKIPLQ"
     gene            complement(3108..3875)
                     /locus_tag="PLY93_06130"
     CDS             complement(3108..3875)
                     /locus_tag="PLY93_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69091.1"
                     /translation="MVIGHLKYIFKVLFLLVLFNFTYSLFAANLESDYRDAVVLFEKR
                     DVVSCEASIAVLEKILEQKTDSLDAQALIAYAYAHHGGMLQQINGSGGEYLNSAYAFA
                     KAALSQQPTNIYARKAILLLKLIGGDIVDVRKQVEKEWSEKETDADIWYFRAVSGDAA
                     AATKSLQEALRKNTSHVWIYSDMAFRALLANDFATAEKWIAALQKQQAEIADVDLLKA
                     VLAIKRSDEKEARLAWDSFTRKAPESVIVDAYKKLKK"
     gene            complement(3904..4878)
                     /locus_tag="PLY93_06135"
     CDS             complement(3904..4878)
                     /locus_tag="PLY93_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRP69092.1"
                     /translation="MKKYFILTTAFAVAVGCGPKVDYEALRNRAKSTFGVLPDKMPGA
                     EKDTPELIALGKALYNDKRLSVNDTQSCASCHAIEGKGGGVDNKKFSPGAKPNTVGVR
                     NTPTVLNAGFHMAQFWDGRAPTLAEQAKGPILNPVEMGMPNEKAVVNKVSKITEYKES
                     FAKAFPKVNNPITYQNLADAIAAFERTLVTHDRFDDFMKGDNKALTKAELDGMKTFMD
                     QGCTSCHMGPLLGGNSYRKMGQAKAYPTKDLGRFDVTKKEEDKFMFKVPSLRNIALTA
                     PYFHDGSAATLTDAVAKMANHQLGKLLGENDVNSIVAFLKTLTDKKRL"
     gene            5094..5309
                     /locus_tag="PLY93_06140"
     CDS             5094..5309
                     /locus_tag="PLY93_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69093.1"
                     /translation="MADKHLVACEQEHEMVTVLKHFKKGGVKENVEAMRNACRSWKKD
                     ASPKYKPKNRVAFYKYLEEKGILGKLK"
     gene            5309..>5526
                     /locus_tag="PLY93_06145"
     CDS             5309..>5526
                     /locus_tag="PLY93_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRP69094.1"
                     /translation="MLQPGKKITASLTGILVSGADSQKVKLSSLYKDKKLILYFYPKD
                     NTPGCTTEAHCFRDNSVALKKAGFNVVG"
BASE COUNT         1612 a         1193 c         1110 g         1611 t
ORIGIN      
        1 cttttcaata atcattatac cgctcgtaac attggttcat acaagaactt gtgtaattgt
       61 aatcctaaac gaaaagaaaa gcaagaattt tttacccact cccccacttc gctaccagat
      121 aattgagcga acaccggctg cacaatcggc ggcctttcca gtttggtgtg gagcgcatat
      181 tgttctgtaa ttttttcagc aaataaaaaa tcggagtgcg acgcgacgac aaacttcacg
      241 tcgtcggtgg gtttaatcca ttgcaaattt tcataccaat tttttttatc ttcgcctgag
      301 tcggggggtt ttatatcgag aacgatatgg acatacggcg gtactttatc gatcgggatt
      361 gatcccgatg tctctaaaag aactctcttt ttttctttat gtagtaattc aaacagtgta
      421 tacacatttt tctgaaggag tggttctcca cccgtgagct ccactaagtc gcatggatat
      481 ttttggatag tttggagaat ttgttcaaat gtttgggttg ttccttcgta atacgaataa
      541 gtcgtgtcgc accaaacgca acgcaaattg caaccggtta aacgaataaa aatgcaagga
      601 tacaacgcat acgagccctc tccttggata ctataaaaaa tttcattgat tttcaaactg
      661 tgcaactctt tagacattga ttctaattat gcgcaaaatt aaagccaccg taaatctctt
      721 tttggttttt ccttttgcgc tttttgccgt tgaaaagaaa tcggcgtatc ttatggcgga
      781 gcgtagcgac acgcactatg aaataatttc ttcaaaaaat acccaactca ttgcgccgat
      841 gggttcgctc ctcaaacctt ttgcggcatg gtatctgttg gagcgcggca tcgacagcga
      901 acaagttatt tattgcccgc cacgccgcaa gcagacaaaa tcgttgcgct gttggataga
      961 cactggtcat ggagcaatga cactcaaaac tgcattggct caaagttgta attattattt
     1021 tctttcgcga tttttgggat taaacttggt agagtatgaa acttggttac aaaataaatt
     1081 cgattggcca atcacgctaa aaattacgaa accagaaaac gtctacggct tcgatttgac
     1141 gggcggaatg gatgtaaaaa aaattctctc tatgtataca aagttattaa acgccagcga
     1201 cgaaggcaat ccacacgcag aaacaattat ggatggttta agccagacct gccaaggaac
     1261 gttacaagac ttttgtagtg ctatcgcaaa aaatagaagc gttcgattta ttgctggcaa
     1321 aaccggtaca attatagacg caggtaaaaa ttacggaatc gcctttcttt atttagaata
     1381 cctaccgcta aaacaaaaaa ttatcttgct ctgttatgaa aaaaacaaga tgggttctca
     1441 agcggcgttg aacgcagtaa caatcttaaa tggctaccgt agaaaaaaat aattctgaaa
     1501 agacatccgc gtcggattgg ttgtcgttct ggggttcaat ccaaaaccca aatcgaatga
     1561 cgctatttat tggttttgtt gtcttgatgt tctttacagt tttcttggga tattttggaa
     1621 tgcgtacttt ggtgcgttcc ttcgattatt atcgtatctc gtttgaagct ccctttatcg
     1681 aagacatccg ccctggtaca aaaattcgct accagggcgc tttagaaatt ggcgaagttg
     1741 tttatatgaa atcaagcgca tcggggcacc gcatcgttgc taaaattcga gacgactttc
     1801 atataccaaa aattggcagc cgtgcgtctt tgagttcttg gggctatttc ggcgcaaaat
     1861 ttatcaacat tgaggttatg cccacaactt tcaatcaaga tttttacact ctcgatacac
     1921 cgatgcctct tgaaaaaatt atcaactcga cgattatgtt tcagcaatac tacgacgcca
     1981 tcaagtcgga aggcggcgaa ctgagtgtgt tagaaaaacg attaagccaa cttaaaaaaa
     2041 ccgttttgga tataaaaaaa acgccttacg tagagcggca ccgtatccgc tcaatagtgc
     2101 gcaatgccgc atcgacaact acacaaatgt acagcgttgt caattcgacc agtgccgatc
     2161 tttacaaaat gttaaagcaa ttaaacgatg ctagcgaata ccttgtctac gatttaaata
     2221 aaaacttgcc gtcgattaaa gctaaaatgg ataaatttga gcaaagcgta accaccgacg
     2281 ggcagagttt tacatctcga ttattgcacg aagaaacaat ttattatctt ttgcttgatt
     2341 atgtgaaatt cacaaatgaa aaactgaatg acatggcgtc tgcgccttat agaattttct
     2401 ttaatcaata atggctgaaa aaattaaact caaagtaaaa tgccacgctt gttcctatgt
     2461 aatggaaggc acggcaaaat atgggaaagg caactaccat cccgaagggc tcgactttgc
     2521 tttcacagcc gtcggtaagc tcgtcgacaa agcaggcgct tcgcgcgtta aaggccaagt
     2581 tgaaattata tgccctaact gtgaaactcg caatcgtttt gatatttagt tttttttctc
     2641 gctctctttt tgcagagtgc actcgtgcaa ttcatttttc ttctgtaaaa ctagcgtggg
     2701 aaaagattac aagcgcctat ggcgacacgc cattaccaaa agagattttt atttgtattg
     2761 ggcgcaatta tttttttgaa ataccgcgtg gctcaacaat gcgctcggtg ttagatagaa
     2821 aaaacaaacg catcttgctg cgcaagacat cgatgcacga cttgacgcac gagcttttac
     2881 atctctatct cgatttacgc tggcaagttc tgccctactc tattagcgaa ccctttgtcg
     2941 ttgcgatgaa caatttcaat tcttgcccca atatccatga aataaaatct tcacttcctg
     3001 aacggtggca acgacgcaaa gagatcgaca cctgtggtgt aaaaaatttg attatcgata
     3061 tactgcaagc atccaccgac gaaagaaaaa aaataccgtt acaataatta cttctttaac
     3121 tttttgtacg catcgacaat taccgactcc ggcgctttac gcgtgaagga atcccaagct
     3181 aaacgcgctt ctttttcatc gctacgcttt atcgccaaca ccgcttttaa taaatccaca
     3241 tcggcgattt ctgcctgttg cttttgaagc gcggcaatcc acttttcagc ggtggcaaaa
     3301 tcgtttgcca gcaatgcacg aaacgccata tcgctgtaaa tccaaacatg cgatgtgttt
     3361 tttcttaacg cttcttgcaa cgactttgta gctgccgccg cgtcaccact caccgcccga
     3421 aaataccata tatcggcgtc tgtttctttt tcgctccact ctttctcaac ttgcttgcga
     3481 acgtcgacta tgtcaccgcc aatgagtttc aaaagtaaga ttgctttccg tgcataaata
     3541 tttgttggtt gttgtgagag cgccgctttt gcaaacgcat aagcggagtt taagtactct
     3601 ccccccgaac cattaatctg ttgcagcata ccgccatgat gtgcgtacgc atacgcgata
     3661 agggcttgtg catccaaaga atctgttttt tgctctaaga ttttttcaag aacggcaatc
     3721 gaggcttcac aagaaacaac gtcacgcttt tcaaaaagta ccacagcatc tctgtaatcg
     3781 gattcgagat tggctgcaaa aagtgaatag gtaaaattaa aaaggacgag aagaaataag
     3841 actttaaaaa tatattttaa gtggccgata accatatttg attatcggca ttttagatgg
     3901 cgattaaagc cgctttttat cggtgagtgt ttttaagaac gctacgatag aatttacatc
     3961 gttttcaccc aaaagtttac cgagctggtg gttcgccatt tttgcaaccg catctgtcaa
     4021 tgttgccgca gagccatcgt gaaagtacgg cgcagttaaa gcgatgtttc tgagcgaagg
     4081 caccttaaac ataaacttgt cttcttcttt cttggttaca tcaaaacgtc ccaaatcttt
     4141 tgtggggtac gctttcgctt gtcccatttt acgataactg ttgccaccca aaagtggacc
     4201 catgtggcaa ctggtgcaac cttgatccat gaaggttttc ataccgtcga gctctgcttt
     4261 tgtcagcgct ttattatcgc ctttcataaa atcgtcgaag cgatcgtgcg ttactaatgt
     4321 gcgctcgaag gcggcaatcg catcggctaa attttggtat gttattggat tatttacttt
     4381 tggaaacgct tttgcaaaac tttctttata ctcggtaatt tttgaaactt tattcaccac
     4441 tgctttttca ttcggcatcc ccatttcaac aggatttaag atgggtccct ttgcttgctc
     4501 tgcaagagta ggagcacgac cgtcccaaaa ctgagccatg tgaaagccgg cgttcaacac
     4561 cgtgggtgtg ttacgcacgc caacagtgtt tggttttgct ccggggctaa attttttatt
     4621 gtcaacacca ccgcccttgc cttcaatcgc gtggcaactt gcgcacgatt gtgtgtcgtt
     4681 cacagaaagg cgtttgtcgt tatagagagc tttacccaat gcaatgagtt ctggtgtatc
     4741 tttttctgca cccggcattt tatccggtaa aacgccaaat gtactttttg cacgattccg
     4801 taatgcctcg taatcgactt tgggtccaca tcccaccgct acagcaaacg ctgttgtaag
     4861 tataaaatat tttttcatag gagataaaac tcacatgata cgtcgggtga caattcaaaa
     4921 aacaacttaa cttggggtag cttagagcga acttgcgcgc caactcgctg agacaatttc
     4981 tttgcacccg ctgcgcgagt tggcgaacag gattcttaac gtattttcat aaaatatatt
     5041 gacggcgttg agcgactcta tgagtctcat aaaaatcttt taaggagaac tcaatggctg
     5101 ataaacactt ggttgcttgc gagcaagaac atgaaatggt aaccgtactc aaacacttta
     5161 aaaaaggggg cgtgaaagaa aatgttgagg cgatgcgtaa cgcatgccgc tcatggaaaa
     5221 aagacgcaag cccaaaatac aaaccaaaga atcgtgtagc gttctacaaa tatcttgaag
     5281 aaaaaggaat tctcggtaaa ttaaaataat gcttcaaccg ggtaaaaaaa ttactgcatc
     5341 gctaacggga attttagtta gcggtgcaga ttctcaaaaa gttaaactct cttctttata
     5401 caaagataaa aaattaattc tctacttcta tcccaaagac aacacccctg gttgcacaac
     5461 cgaggcgcac tgtttccgcg acaacagtgt tgcgttaaaa aaagcggggt ttaatgttgt
     5521 gggctg
//
LOCUS       DAOMPD010000140         5509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6238_length_5509_cov_3.074624, whole genome shotgun sequence.
ACCESSION   DAOMPD010000140 DAOMPD010000000
VERSION     DAOMPD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6238_length_5509_cov_3.074624"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..889
                     /locus_tag="PLY93_06150"
     CDS             167..889
                     /locus_tag="PLY93_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRP69095.1"
                     /translation="MKVSLLTFFIFTSSLLAVESIPDEAQRAFEYLNHVRANPSLFSK
                     EVNVDLSHAAKRPALLWDNRLAASAQEKAEDLANRAYLDHFSPEGIGPGELLHKHGYY
                     LPLSWPDSSEFNYVESISAGSSRSGVNHIINLLYDHGVPDSSPKANHRKHLLGMNKFW
                     ASHVHIGIGFAYNPKAKFKGYLVVHTGVPGVPFVTVTGSLNTPNPIVSTAQTSEIWYN
                     RNSIDFYIIFFITSGCAFSILF"
     gene            783..2618
                     /locus_tag="PLY93_06155"
     CDS             783..2618
                     /locus_tag="PLY93_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69096.1"
                     /translation="MFQPLRLRKFGIIEILSIFISFSSLQAGVHLASCFEPTEAGQKA
                     LSIFLRHNTNHTLKPYLRHFCDPKEMAIKSHALAALDNIRADGQSKDWPIGSFTFVDK
                     RDNVEQYDKTKPATGAYGHIDLLQFNAFYAGDRLFLTYVTANKSPASDVTYWLQLYDA
                     ENQRLYDFIFGETSYVNLYSAGKYQETKPLSADEVQFKHGALTEVIFNAKNFALPPQL
                     RGHAVVFSATRNLVNSTSTFILDDLAQTRIKSAAQVLTHISDVAVADTGTLPLAIALS
                     ESFAYENSTPDIQVRVLNDARTLYTYAQTHAFDFSILSFEAQLAWSNRSMMWGGLAGL
                     YLDTHGKINAEAYDFMFLSPARLPEIRNALEANGIDANWDDEKIFDEIDKFALAKNRY
                     RWKPDEVCRWAEVYPEHADFQRLCIETRNDTANGGQIMTVNGKPIYIWAIMAPNYQWF
                     SIRTNGFFFGPCGDVAVVTMAGLKAFGIADFALFRKLGETDDFTHTFAGFYDKKRHVW
                     RSITNQLPQPNQKGTFTKPYLQFSLPISSPAMPVWHKLTGVQGVVFWINERSPVHSMS
                     FSEMVKKLKVGFTNKEIRKLITSENNSISQGETQTNGLLFNLSKE"
     gene            complement(2706..3143)
                     /locus_tag="PLY93_06160"
     CDS             complement(2706..3143)
                     /locus_tag="PLY93_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRP69097.1"
                     /translation="MWCIFKYNTSPFAKIVKYFLGGSIVTTSQFLSLFIFVEFLDWKN
                     VPQQSIANASAIAVSILSGYLIHSRFTWPILTPSPKNKRIRFLKFYFVSLASLFLRAL
                     VFFALSLTGINYLSNAAIGVVLVVFLNFVVYEKFVFRESNFKT"
     gene            complement(3120..3821)
                     /locus_tag="PLY93_06165"
                     /pseudo
     CDS             complement(3120..3821)
                     /locus_tag="PLY93_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3879..5060)
                     /locus_tag="PLY93_06170"
     CDS             complement(3879..5060)
                     /locus_tag="PLY93_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HRP69098.1"
                     /translation="MDVNADNTRFYLDLTPAPSLVEALSGEYTQALPKNWCIVITDIV
                     GSTKAIEEGRYKDVNTAGGLAAMALSNVFKTLEFPFIFGGDGATFLIPNEVRETTESV
                     LIDTAYKVKEFFDLELRVGIVPEDVIRKAGYEVRVGKITVSPYYTQAVILGDGVEYAE
                     KLVKHDTAFDVTAHDSNGIKANFFGFSCRWHDVPSVQGETVALILRFNAAKETDRVRH
                     LNEALASIEKFYGSEAEYHPIREEAMQFAYSKKVLWREAAARVQKKRGIRFLLLRWQI
                     FVEISVAWLAHTLSIPLKIYHYRLNKIKWYNRVSSDYKKFDGMLKMVLSGTPEARAGL
                     QRYFERWLREGRINYGIHVSNRAILTCLLHTGTAQEVHFVDAADGGYAMAAKMLKKQL
                     G"
     gene            5147..>5509
                     /locus_tag="PLY93_06175"
     CDS             5147..>5509
                     /locus_tag="PLY93_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRP69099.1"
                     /translation="MYGHKKTKPRVIPLRVILLLLVFLFQPLEALNLTVRDIARISWH
                     RENQVSGYGVVVGLKGSGDSRSELALETLKKNLYNRGIDIGEKTFTARNIAAVMVTAI
                     LPTHSRAGDPVDVWVSSIG"
BASE COUNT         1583 a         1220 c         1137 g         1569 t
ORIGIN      
        1 accccccaat tgcggcgtag catttgtcag gtatgtccgt gggttagaca taaagccgat
       61 gtgttttgat tgaccccccc aagtttttag ctatatctcc cgcatcatga tttaggataa
      121 ttttttatcg atggtgtacg tctaataagc atataaaaga aagattatga aagtatcgtt
      181 attgacattt tttatattta ctagtagtct tttagctgtt gagtctatac ccgacgaagc
      241 tcagcgcgct tttgaatatc tgaaccatgt cagggctaac ccatctttgt tctcgaaaga
      301 agtgaacgtt gatttaagcc atgcggccaa acgccccgcc cttctttggg ataatcgttt
      361 agcggcctcg gctcaagaaa aagccgaaga cttagccaac agagcttatc tcgatcattt
      421 ttcgccggaa gggattggtc caggtgagct gttgcataag catggttact accttcctct
      481 ttcatggccg gattcgagcg agttcaatta tgtcgaatcc atttctgcag gtagttctcg
      541 gtcgggagta aatcatatca tcaatcttct gtacgatcac ggagtacccg attcaagccc
      601 aaaagcaaac caccgcaagc acctattggg tatgaataaa ttttgggcat cgcatgttca
      661 tattggtatt ggtttcgcgt ataatccaaa ggctaagttt aaaggttact tggttgtgca
      721 taccggcgtt ccaggtgttc catttgtgac tgtcacgggt tctcttaata cgccgaatcc
      781 aattgtttca accgctcaga cttcggaaat ttggtataat agaaattcta tcgattttta
      841 tatcattttc ttcattacaa gcgggtgtgc atttagcatc ttgttttgag ccaaccgaag
      901 ccggacaaaa agctctttcc atttttttgc ggcacaatac aaatcatact ctaaagccat
      961 acttacgcca tttttgcgat ccaaaagaaa tggcaatcaa gtcgcacgct ttggccgctc
     1021 tcgacaatat ccgtgccgac ggccagagta aagattggcc tattggttcg tttacattcg
     1081 tcgacaagcg agataacgtt gagcaatacg acaaaactaa gcctgcgaca ggcgcatacg
     1141 gacatattga tttattgcaa tttaacgcat tttatgcagg cgatcgactt tttttaactt
     1201 atgtaacagc gaataaatct ccagcgagtg atgtcacata ctggttgcaa ctctacgacg
     1261 cagaaaatca gcggctctac gatttcatct ttggtgaaac aagttatgta aatctctatt
     1321 ctgccggaaa atatcaggaa acaaaaccgc ttagtgcaga tgaagttcaa tttaagcatg
     1381 gcgcactcac cgaggttatt tttaatgcta aaaattttgc gctaccgcct cagttgcgtg
     1441 ggcacgcggt cgtcttcagt gcgacacgca atcttgtaaa ctcaacatca acttttatat
     1501 tagacgattt agcgcaaacc agaattaaat cagcggcgca agttcttacg catatctcag
     1561 atgttgctgt tgcagacaca ggaacattac ctcttgccat tgcgctcagc gaaagctttg
     1621 cttatgaaaa ttctacacca gatattcaag tacgcgtgtt gaacgatgcg cggacgcttt
     1681 atacgtatgc acaaactcat gcctttgatt tttcaatatt atcttttgaa gcgcagctcg
     1741 catggtctaa tcgctcaatg atgtggggtg gtttagcggg cctatacctt gatacacatg
     1801 gtaaaatcaa tgcggaagcc tacgatttta tgtttctcag ccccgcaaga cttcctgaaa
     1861 ttagaaatgc tcttgaagca aacggcattg acgcaaattg ggacgatgaa aaaatctttg
     1921 atgaaatcga caaatttgcg cttgcaaaga atcgttatcg ttggaagccc gatgaagtat
     1981 gccgttgggc ggaggtgtac cccgaacacg ctgatttcca acgcctttgc atcgaaacac
     2041 gaaatgatac agcaaacggc ggacagatta tgaccgtcaa tggtaaacct atctatatct
     2101 gggcgattat ggcgcccaac tatcaatggt tttcgatccg cacaaacggc ttttttttcg
     2161 gaccatgcgg tgatgtagcc gttgttacca tggcaggact taaagcgttt ggtatcgccg
     2221 attttgcttt atttcggaaa ttgggtgaaa cggatgattt tacacataca tttgcaggat
     2281 tctatgacaa aaagcggcat gtatggcgca gcatcactaa ccaattaccg cagcccaatc
     2341 aaaagggaac tttcacaaag ccctatttgc aattttctct ccccattagc tcaccagcaa
     2401 tgcctgtatg gcacaaactg acaggggtac agggtgtagt attctggata aatgaacgta
     2461 gccctgtaca ctcaatgtca ttttctgaga tggtgaagaa actaaaagtt ggttttacaa
     2521 ataaagaaat taggaaattg ataacctccg aaaacaactc aatttcacaa ggtgaaacgc
     2581 aaactaatgg gctattgttc aatttgagca aagagtaggc aagtggtcat gtgtggactg
     2641 tgaagcttcc agttttttat ccgcccttat gtagttgaaa attttttaat tgtatagata
     2701 attctctacg ttttaaaatt agattctcta aacacaaact tttcataaac aacaaagttc
     2761 aaaaagacta ctaaaacgac gccaatagct gcattagata agtagtttat gccagtgagc
     2821 gagagcgcaa aaaaaacgag ggcgcgaaga aataaagaag cgagagaaac gaagtaaaat
     2881 tttaaaaagc gaattctttt attttttggg gaaggagtta aaataggcca tgtaaatctg
     2941 gagtggatta gataaccgct caaaatagaa acagctattg cagaggcatt ggcaatactt
     3001 tgctgtggaa catttttcca atctaaaaat tcgacaaata taaataacga taaaaattgc
     3061 gatgtcgtta caatgctgcc acctaaaaaa tattttacaa ttttagcgaa cggcgacgtg
     3121 ttgtatttga aaatacacca cagcgcacga aaggcgtctt tcccacgaat ttttttacct
     3181 tcatcgtaag tgcgtccatg ataagatata cccacttcat agagcctaac tttatacgaa
     3241 cgtataaatt cgccgagttt tgctgtgact tccggttcaa aaccaaaccg cttttcaaca
     3301 agatttagac tttgcaaaat accgcgcttg aataatttat agcctgtctc catgtctgtt
     3361 aaatttaaat cactaaacat attggaaatg agcgtcaaaa gtctgttggc aaacgcatgc
     3421 cagtaataaa taattcgatg agggcgactg cctacaaaac gagaaccata cacaacgtcc
     3481 gctttacccg ttagcattgg ttctaaaaga attggatatt cgtgtggatt gtattccaaa
     3541 tcggcatctt ggataacaac ccaatccccc gttgcaagcg caatacctga atgaattgct
     3601 gcgcctttac cttgattttt ctcatgcaaa atgagtttta tgtttttctt cttaaattgt
     3661 aatttcaaaa tttcgcgtgt gccatccgtt gagaaatcat cgacgacaat tatttctttt
     3721 tttatgctat gcggtaactt tgccgacttc acttttgata acaatgctaa aatcgtctgt
     3781 ttttcattaa aaacaggtat gatgatcgac aagcacttca tggattattg gataggctta
     3841 ttttaagttt ttatatcaac tcccattgca taatccactc accccagctg cttcttcagc
     3901 attttagccg ccatagcgta accgccgtcg gcggcatcga caaagtgtac ctcttgcgcg
     3961 gtacctgtgt gtaaaaggca ggtcaatatc gcacggttgg aaacgtgaat gccgtagtta
     4021 atccgcccct cacgcaacca tctttcaaaa tacctttgaa gacctgcacg cgcctcggga
     4081 gtaccgctca agaccatttt gagcattcca tcaaattttt tataatcgga ggaaactcga
     4141 ttataccact ttattttatt gagtcgatag tgataaattt taagaggaat agataacgta
     4201 tgcgcaagcc atgcgacact aatttcaacg aaaatctgcc aacgcaaaag taaaaaacga
     4261 atgccgcgct ttttttgcac ccgcgccgcc gcttcgcgcc agaggacttt tttggagtat
     4321 gcgaactgca tcgcctcttc gcgtatcgga tggtattctg cctcgctacc ataaaatttt
     4381 tcaatcgatg ccaacgcttc attaagatgg cgcactcggt cggtttcttt tgctgcatta
     4441 aaccgtaaga tgagcgcgac agtttcgcct tgcacgctcg gcacatcatg ccacctgcac
     4501 gagaatccaa aaaagttggc tttgataccg ttgctgtcgt gcgccgtaac gtcaaacgct
     4561 gtatcgtgct tcactaactt ttccgcgtac tctacgccgt cgcccaaaat caccgcttgc
     4621 gtataatacg gtgaaacggt aattttaccc acgcgcactt cgtaccccgc tttacgaatg
     4681 acatcctcgg gcacaatacc cactcgtagt tccaaatcaa aaaactcttt cactttatac
     4741 gcggtatcga tgagcacact ttctgtcgtt tcacgaactt cgttggggat taaaaaagtg
     4801 gcaccatcgc cgccaaaaat aaaaggaaac tctaatgttt tgaaaacatt actcaacgcc
     4861 atggcggcga gtccaccggc ggtgtttaca tctttatacc gcccttcttc aatcgctttt
     4921 gtcgagccaa caatatcggt gatgacgatg caccaatttt ttggcaacgc ctgcgtgtat
     4981 tcaccactca acgcctctac cagcgacggt gcaggagtga ggtcaaggta gaagcgggta
     5041 ttgtcggcat taacatccat aacgctttga aaaaaaatta tatatgtcgt aaagtcattg
     5101 agttttattg gagttagccg ataatggaaa aggcgaggaa tctattttgt acggccataa
     5161 aaaaacaaaa ccgcgtgtaa tcccactgag agtaatttta ttgttactcg tctttctttt
     5221 tcaaccgttg gaagcgctca atctaaccgt acgcgacatt gcacggatca gttggcaccg
     5281 cgaaaatcaa gtgtcggggt acggtgttgt cgttggcctc aaaggtagcg gcgattcgcg
     5341 ttcggaactc gcgcttgaaa cgctgaaaaa aaatctctac aaccgtggga ttgacatcgg
     5401 cgaaaaaaca tttactgcgc gcaacattgc cgcagtgatg gtgaccgcaa ttctacccac
     5461 gcactcgcgc gcgggggatc ctgtcgatgt gtgggtgtct tcgatcggc
//
LOCUS       DAOMPD010000141         5505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6245_length_5505_cov_3.567890, whole genome shotgun sequence.
ACCESSION   DAOMPD010000141 DAOMPD010000000
VERSION     DAOMPD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_5505_cov_3.567890"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLY93_06180"
     CDS             complement(<1..896)
                     /locus_tag="PLY93_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRP69100.1"
                     /translation="MNATELLDIISAGETSKVQFKREIDNDDSIAAELIALSNSKGGV
                     ILFGVEDKKGTVMGLEYEQLQSYNNRIATIANEKIKPQIFLYTEVVSVLSESRKKKIL
                     VVEVSEGIAKPYKDKNGVIWIKQGADKRKLTDNSEIIRLFQQSGILYTDEMVIPQTSV
                     DDINLIKVEQYIKTIQKRDADDQVEITETLLKNLNILNDGRLTLGGLLFFAKNPQKYR
                     PAFCVKAISFFGNSIGGTDYRSSQDIEGTIPEIFEDTMRFFKTNLHHIQAGQNFNSIG
                     KLEVSQIAFDVLLQNALVHRDYS"
     gene            complement(1173..2231)
                     /locus_tag="PLY93_06185"
     CDS             complement(1173..2231)
                     /locus_tag="PLY93_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRP69101.1"
                     /translation="MGIFKSIENRKIRFALVGAGRIAANHFGAIEKLANECELVDVCD
                     VDKAALDAAVSKTGAQGHLSLTAMLQSTTADAVILTTPSGLHPRQAIEVAQSGRHVVS
                     EKPMATQWQDGLAMVRACDDAGVRLFVVKQNRRNATLQLLKKAVTQGRFGRIYNVALN
                     VFWTRPQDYYDAASWRGKWEFDGGAFMNQASHYIDLLGWLIGPVESVMAYTATQARNI
                     EAEDSGVAAIRWRNGAMGTLNVSMLTYPKNLEGSITILGEKGTVRVGGVAVNEIQEWQ
                     FVDKRPEDDEIKNANYATTSVYGFGHPLYYDNVIQTLRGKAEPETDGREGLKSLELLI
                     AMYRSARDGVRVALPLER"
     gene            complement(2252..3550)
                     /locus_tag="PLY93_06190"
     CDS             complement(2252..3550)
                     /locus_tag="PLY93_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRP69102.1"
                     /translation="MNTAENQKIAVVGLGYVGLPLAVEFGKKRAVLGFDINAARIAEL
                     KVGNDKTLETTSEQLTQAKYLSFSDKLEDLKTCTIFIVTVPTPVDHVNRPDLTPLQKA
                     SHTVGTALKPGDIVIYESTVYPGATEEVCVPVLEKTSGLIFNKDFFAGYSPERINPGD
                     KVNTLTKIKKITSGSTPEVANTIDALYSSIIIAGTHKAASIKVAEAAKVIENTQRDLN
                     IALVNELSVIFDRIGIDTLDVLEAAGSKWNFLPFRPGMVGGHCIGVDPYYLTHKAEEV
                     GYNPQVILAGRRINDNMARYVARATIKRMVKNGINVAQATVGVLGVTFKENCPDIRNS
                     KVADLIKELKTWGVQVVAIDPWASADEVQHEYGFSLGEITPSAPVDSLVIAVGHNEFR
                     TLSAQTLRSYVRTERPVIADVKSLYKREELAAQGFTVFRL"
     gene            complement(3561..4136)
                     /locus_tag="PLY93_06195"
     CDS             complement(3561..4136)
                     /locus_tag="PLY93_06195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRP69103.1"
                     /translation="MSVTIHPSAIVDEGAQIGEGSRVWHFVHVCGGAKIGKGVSLGQN
                     VFVGNKVTIGDNCKIQNNVSVYDNVHLEEGVFCGPSMVFTNVYNPRSLIERKNEYRDT
                     LVKKGATLGANSTIVCGITIGEYAFVGAGAVVNKDVPAYALMVGVPAKQIGWMSEFGE
                     QLDLPLEGDGEAACKHTAARYVMKNGVVKKE"
     gene            complement(4161..4817)
                     /locus_tag="PLY93_06200"
     CDS             complement(4161..4817)
                     /locus_tag="PLY93_06200"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRP69104.1"
                     /translation="MEAIDYKKLYALQDAVLQEVFVTEHEFYLTGGTCLSRFYVAKRY
                     SDDLDFFTNRSARYSFSLKSIIAALEKKFSIKTEAQSKDFARFILNDQLQVDFVNDTA
                     FHYKDIKVTESGYLIDNVENIFANKLTAIIGRDSAKDVFDIILIAKYFNFSYQDILTA
                     AQKKMAFAIEDLIVRLKEFPLAWLNEVKPIEPFTITGKDIETVCRDMVEGKDNSLFVG
                     "
     gene            complement(4805..5122)
                     /locus_tag="PLY93_06205"
     CDS             complement(4805..5122)
                     /locus_tag="PLY93_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69105.1"
                     /translation="MLVPEDLERIVKECYWDSHTSPERIVSVAKGSDLRLKKRLFEKI
                     LLNSTNLFRDLTVFNNGDLAGLIESYTVPSFNKEYTFRRKNLAEVYFLHKPLLIPELQ
                     WRR"
BASE COUNT         1505 a         1357 c         1051 g         1592 t
ORIGIN      
        1 gaatagtctc tatgcaccaa agcattctgc aataacacat caaatgcaat ttgcgaaact
       61 tctaatttgc caatcgaatt aaaattctga cctgcctgaa tatgatgcag atttgttttg
      121 aaaaaacgca ttgtatcttc aaaaatctca ggaatagtac cttcaatgtc ttgactgctt
      181 cgataatcgg ttccaccaat actgtttccg aaaaatgata ttgctttcac gcaaaatgcc
      241 ggacgatatt tttgcggatt tttagcaaaa aataaaagcc ctcccaatgt aaggcggcca
      301 tcattaagta tgttcaaatt ttttaataga gtctctgtta tttcaacttg atcatctgca
      361 tctctttttt gaattgtttt gatgtattgc tctaccttta taagattgat gtcatcaaca
      421 gaggtttgtg ggataaccat ctcatcagta tataggatgc cactttgctg aaacagcctt
      481 attatttcgc tattatcagt taattttcgt ttatcagctc cttgctttat ccaaattaca
      541 ccatttttat ctttatacgg ctttgcaatc ccttcagaaa cctctacaac caaaattttc
      601 ttttttctcg attctgaaag cactgaaaca acttcagtat aaagaaatat ttgtggcttt
      661 attttttcgt tggcaattgt tgcaattcta ttgttgtatg actgcaactg ttcatactct
      721 aaacccatca ctgtgccttt tttgtcctca acaccgaaca aaatcactcc cccttttgag
      781 ttagacaagg ctattagttc ggcagcaata gaatcatcat tatcaatttc gcgcttaaac
      841 tgtactttac tcgtctcgcc agcgctaatg atatctaata attccgtcgc gttcatatca
      901 tttttcacta tgcacatgct tgagtgccga aaggttcaac atataatcgc accacctttc
      961 attaaagttt taagattaat tatcttcacg ccaccctata cttctgctag ctctcgatta
     1021 tcgtcaaaga aaatattagg ccagtctgcg gttgctcgtc gaatccccgt acctaaacca
     1081 cgatagggta aggcactctt aagagcaaac gaagctaaaa aagaatcggt gtttgtagca
     1141 tccaccttgt aaagtattgt acccaatttt acttacctct ccaacggcaa cgccacgcgc
     1201 acgccatcgc gtgcagagcg gtacatcgca attaaaagct caagactctt gagcccctcg
     1261 cgtccatcgg tctcgggttc ggccttgcca cgcagcgttt ggatgacgtt gtcgtagtaa
     1321 agcggatgac caaaaccata gacgctcgtg gttgcgtagt tggcgttttt aatttcatcg
     1381 tcttctggcc ttttgtcgac aaactgccat tcttggattt cgttcaccgc cacgccacca
     1441 acacgcaccg tgcccttctc gcccaaaatg gtgattgaac cctcaaggtt tttagggtac
     1501 gttaacatgc ttacgtttaa cgttcccatg gcgccgttac gccagcgaat tgctgccaca
     1561 ccgctgtctt cggcttcaat attacgtgct tgggttgcag tgtacgccat gacgctttcg
     1621 acgggaccaa taagccaacc taataaatcg atatagtggc tcgcttggtt cataaaagcg
     1681 ccaccatcga attcccattt accgcgccac gatgcggcat cgtaataatc ctgcgggcgg
     1741 gtccaaaaga cattgagagc cacgttgtag atacggccaa aacgcccttg cgtcaccgct
     1801 tttttcaaaa gttgtagcgt ggcgttgcgg cggttttgtt tcacaacgaa aagacgcacc
     1861 cctgcgtcat cacaagcacg caccatagca agtccgtctt gccactgggt cgccatgggt
     1921 ttctcgctca cgacgtgcct gccgctttgg gcaacttcaa tcgcttggcg gggatgcaaa
     1981 ccgctggggg tagtgagaat gactgcgtcg gcagtcgtac tttgcaacat cgcagttaaa
     2041 gacaaatgcc cttgcgcacc tgttttgctg accgctgcat ctaacgctgc cttatcgaca
     2101 tcgcacacat cgacgagctc gcattcgttt gcaagttttt caatcgcacc aaagtggttg
     2161 gctgcaatac ggcctgcccc caccaaagca aaacgaattt ttcgattttc aatactttta
     2221 aatattccca tagttaatcc ccgctattat tttataaccg aaaaacggta aaaccttgtg
     2281 cggcaagctc ttcgcgttta taaagacttt ttacgtcggc aatgacaggc cgctcggtac
     2341 gtacataact acgcagtgtt tgagcactga gtgtacggaa ttcattatga cccacagcaa
     2401 tgaccaaact atcgacagga gcactgggag taatctcacc caaactaaac ccatattcgt
     2461 gctggacttc atccgcactt gcccaagggt ctatagcaac aacttggacg ccccatgttt
     2521 tgagttcttt aattaaatcg gcaactttac tgttacggat gtcggggcag ttttctttga
     2581 aagtaacgcc aagaactccc actgttgctt gggcaacgtt gatgccgttt ttgaccatgc
     2641 gtttaatggt cgcacgcgcc acataccgcg ccatgttgtc gttaatccgg cggcctgcca
     2701 aaatcacctg tgggttatac cccacttctt cggctttatg cgttaagtag taaggatcca
     2761 ctccaataca atggcccccc accatccccg gacggaatgg taaaaaattc catttactgc
     2821 ccgccgcttc gagaacgtca agcgtgtcga taccgatgcg atcaaaaata acagaaagct
     2881 cgttcaccaa agcaatgttc aggtcgcgtt gagtattttc aatcaccttc gccgcttcgg
     2941 cgactttaat cgacgccgcc ttatgcgtgc ctgcgatgat gatgctgcta taaagcgcat
     3001 cgatagtatt agcaacctcg ggagtactac cactcgtgat tttttttatc ttcgtcagcg
     3061 tattgacttt gtcgccaggg ttaatccgct cggggctata gcccgcaaaa aaatctttat
     3121 taaagataag ccctgaggtt ttctcaagta cgggcacaca cacctcttcg gtagcccctg
     3181 ggtacactgt agattcgtaa ataacgatgt cgcccggttt gagggctgta cccaccgtgt
     3241 ggctggcttt ttgcagcggc gtgaggtcag ggcgattgac atggtcgaca ggcgttggca
     3301 cggtaacaat aaaaatcgta cacgttttta aatcttctaa cttatcgcta aaacttagat
     3361 attttgcttg tgtaagctgt tcgcttgtgg tttcgagtgt tttatcgtta cctactttaa
     3421 gttcagcgat acgtgcggcg tttatgtcaa aacctagaac ggcacgtttt ttaccaaact
     3481 caacggccaa aggtaacccc acatacccca aaccgacgac cgctattttt tgattttctg
     3541 ctgtattcat aaaaattaaa ttattccttt tttaccacac catttttcat aacataccga
     3601 gcagcggtgt gcttgcacgc tgcctcgcca tctccttcca acggtaaatc caactgttcg
     3661 ccaaactcgc tcatccagcc aatttgtttc gcaggcacac ccaccatgag cgcgtacgca
     3721 ggcacgtctt tattcaccac cgctcctgcg ccaacgaacg catactcgcc aatggttatt
     3781 ccgcacacaa tggttgaatt ggctcctaac gttgctcctt ttttaactaa agtatcacgg
     3841 tattcgtttt tgcgttcgat gagcgaacgc gggttataga catttgtaaa gaccatactc
     3901 ggtccacaaa acacaccttc ttctaagtga acattatcgt acacagaaac gttattctgg
     3961 attttgcaat tgtctccaat ggtaacctta ttgcctacaa aaacattttg ccccaacgac
     4021 acgcctttac caattttcgc tccgccgcat acatgcacaa aatgccaaac acgcgaacct
     4081 tctccaattt gcgcaccctc gtcaacaatg gcgcttggat gaatagtaac actcataaaa
     4141 atctccttaa tctcaatcca ctaccccaca aacagcgaat tgtctttacc ctcaaccatg
     4201 tcgcggcaaa ctgtttcaat atcctttcca gtaatggtaa aaggctcaat gggtttaacc
     4261 tcgtttaacc acgccagagg aaattctttg aggcgcacaa ttaaatcctc tatcgcgaac
     4321 gccatttttt tttgagctgc ggttaaaata tcttggtaag agaaattgaa atacttggca
     4381 atgagtataa tatcaaacac gtctttagca ctatccctac caataatagc agtgagttta
     4441 ttggcgaaaa tattttctac attatcgatg agatagccgc tttcggtaac tttaatatct
     4501 ttgtaatgaa atgctgtatc gttcacgaaa tccacttgca gttgatcgtt tagaataaaa
     4561 cgcgcaaagt ctttgctctg cgcttcggtt tttatggaaa attttttctc taaagcagct
     4621 ataatacttt taagagaaaa actataacgc gccgacctgt ttgtaaaaaa atctaaatca
     4681 tctgaatagc gttttgctac atagaaacgg cttaagcaag tgccaccggt taaataaaat
     4741 tcgtgctctg ttacaaacac ttcttgcaaa acggcgtctt gcagcgcgta taacttttta
     4801 tagtctatcg cctccattgt agttcgggga ttaacaacgg tttatgtaaa aaatacactt
     4861 cggctaaatt ttttctgcgg aatgtatatt ctttattaaa agaaggcaca gtgtatgact
     4921 caatgaggcc cgctaagtcg ccgttattaa aaacagttaa atctcgaaac aaatttgtgc
     4981 tgttgagcaa aattttttca aaaagccttt tctttaagcg aaggtcgctc cctttagcca
     5041 cgcttacaat tctttcgggg gatgtatggc tatcccaata acattctttg acgatacgct
     5101 caaggtcttc tgggacaagc atcctttata cccaacagga aaaatacgat tcaaaacaca
     5161 gcttcgccgc gtcagtcaca gactgactca aacaacccat gttgagagaa agacaaacac
     5221 tcatgtgttc aatcattgaa cagatgcgtg tcaaaacctg caccgtcaat gaaaaaagta
     5281 gaataatgca taactatcaa gagtgagcta aatataccgt caataaatag aaaggaataa
     5341 aagcctgtgc gccaactcgc gcagcgtgca taaagaagca atttttttgc aaatttttgt
     5401 acacggttcg ctcaaaagcg cgaaatttta cacaaattcg gacgtcttcg ccccgtgcag
     5461 agcaaattgg aacaatttgc cgtcggccaa agagccaccg ccctc
//
LOCUS       DAOMPD010000142         5493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6262_length_5493_cov_2.348658, whole genome shotgun sequence.
ACCESSION   DAOMPD010000142 DAOMPD010000000
VERSION     DAOMPD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6262_length_5493_cov_2.348658"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLY93_06210"
     CDS             complement(<1..499)
                     /locus_tag="PLY93_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP69106.1"
                     /translation="MKTAQRNFFFKVRFILFTVLFFFAACQSLPVQGEKNTVVTHDGW
                     ALTIEHLPSLSPQVKRKYPILICHGLAANRQYFKLKDDDSLVRALQNAGYDVWLMDLR
                     GRNDAGETGFFFGKHTYKYDIDDYITQDLDAAIRFVQKETGAPKINYMGHSMGGIIMH
                     ARLGSY"
     gene            complement(496..870)
                     /locus_tag="PLY93_06215"
     CDS             complement(496..870)
                     /locus_tag="PLY93_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69107.1"
                     /translation="MLQGTFEFMMGDRFGLRGTAGIPLSKNISSMEYYPMTLGGAFHL
                     FPRFWFDLYMGAESGFVHFSTNTLSASWSTRITPFVGTTLYYWGVFFLEGEAGYSVWQ
                     YAKSAALDLSAPTYRVRLGFYL"
     gene            complement(851..1027)
                     /locus_tag="PLY93_06220"
     CDS             complement(851..1027)
                     /locus_tag="PLY93_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69108.1"
                     /translation="MKKICTTLCLLSFTTLFAIAPNQMGDDSNDGIQQSNLKLSLVAR
                     KSYRISCALAARHV"
     gene            complement(1089..2168)
                     /gene="rfbB"
                     /locus_tag="PLY93_06225"
     CDS             complement(1089..2168)
                     /gene="rfbB"
                     /locus_tag="PLY93_06225"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRP69109.1"
                     /translation="MKILITGGAGFIGSAVVRHIIQHTDDAVVNVDKLTYAGNLESLA
                     GVSQNPRYAFERVDICDTKETARVFAEHKPDRVMHLAAESHVDRSIDGPAAFIQTNIV
                     GTYTLLEAARAYIANAEESVRKNFIFHHVSTDEVYGDLPHPDETSDYKNFLFTEKMAY
                     APSSPYSASKASSDHLVRAWRRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALELK
                     PLPVYGKGDQIRDWLFVEDHARALYTVVTRGTVGATYNIGGHNEKKNLTVVETICSLL
                     DTLRPRADGRSYKDLIAYVKDRPGHDRRYAIDAQKIAHELGWKPQETFESGLKKTVEW
                     YLANAEWCRRVQDGSYQRERLGKIY"
     gene            complement(2165..>2340)
                     /locus_tag="PLY93_06230"
     CDS             complement(2165..>2340)
                     /locus_tag="PLY93_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HRP69110.1"
                     /translation="NFKSTKKKLAYAYILTPSGVEQKARVTIGFLKRKMREYDELKIL
                     VEELQKEVELVKS"
     assembly_gap    2341..2383
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2384..2501)
                     /locus_tag="PLY93_06235"
     CDS             complement(<2384..2501)
                     /locus_tag="PLY93_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRP69111.1"
                     /translation="MDDEIQHRLFKLIEENPTINQRELSQHLGMSLGKVNYCI"
     gene            complement(2523..3164)
                     /locus_tag="PLY93_06240"
     CDS             complement(2523..3164)
                     /locus_tag="PLY93_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69112.1"
                     /translation="MEKSILISEIKEILVTENDLSYLEKIDDKTIEKIHKEIQGYIQR
                     TEELQKPVFKVMAVATQFIPNFIIAKLAHDYLTPYIIAQVVIYMDPKAAAKIGKSLRI
                     DYMGQVALYANPEVTARVGNLMEVNITAQVVKDLVTKNFTGKLGELADLLDDKVLLDI
                     VNALRDGKIVGNIANAMMNHEKIVRIAQQMPADIRNGAKMQLQTLGNKLAEQF"
     gene            complement(3201..4052)
                     /locus_tag="PLY93_06245"
     CDS             complement(3201..4052)
                     /locus_tag="PLY93_06245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRP69113.1"
                     /translation="MLDNTPSRTAEIVALIRALENAYYGEKSIMHDKYAVRFLSEFYT
                     GIFNRLSILPDFVSFFVNAASLGLFDFISVRHAFMDKYVKDLAPQMPVVLLGAGYDSR
                     ALRLNDSLKHGIWEFDFPATQRRKKFYLRNEKTRGAEVHYYEVDFMKESLAEIFTHAK
                     LEKKPTLVLWEGVSMYVTKEVVCSTIRAVRDYFGEGSYLLYDYWQTLVDHPFKSILHA
                     ILPYAMDLIYNEKFTYGATVDDMRKLALDCGAKKFESFNGKQMTTKLSLTSRLPLTSA
                     AVAVLNF"
     gene            4094..4255
                     /locus_tag="PLY93_06250"
     CDS             4094..4255
                     /locus_tag="PLY93_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69114.1"
                     /translation="MQFPFFQKKKPSHSVEDQTELLSKLLTKYRNAETKYGKKFFDKT
                     KLDERIIRF"
     gene            4215..4889
                     /locus_tag="PLY93_06255"
     CDS             4215..4889
                     /locus_tag="PLY93_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69115.1"
                     /translation="MIKPNSTNASSDFERFILDEMQFYKAMEEKAIEEEARRLRQEEA
                     DAHMRAIMAENDARIEHYEDTFFHPLATLECRRIVSAVSSLFPEVEEELRYVYQGRKE
                     WQEMKFNLSDLERFLYKPPMRLTAFLAHYINEIQKWGEGGREEADRKFLQTAAMCLSA
                     ISNGITTSIDHLTEKDKAIAEKIRTRLDTVVDDFRLGDLAQHGLKRKSASGDPHNHFT
                     KMTTAL"
     gene            complement(4842..>5493)
                     /locus_tag="PLY93_06260"
     CDS             complement(4842..>5493)
                     /locus_tag="PLY93_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRP69116.1"
                     /translation="PEWAAVEIPQHLTYEEAATLPCAALTAWSALVTLNHINPGEYVV
                     VLGTGGVSIFALQFAKIMGAKVIATSSSNEKLKRAGELGADFFINYKEKPDWAKEVRK
                     ITQMRGADHIVEVGGAGTLEQSIRAVRMDGVVSLIGVLAGSSKELNLLPILMQNVRMQ
                     GVLVGGREGFEAMCTAVGETQMKPVVDNVFGFDELPNALRYLKSGSHFGKVVVRVA"
BASE COUNT         1370 a         1304 c         1331 g         1445 t
ORIGIN      
        1 cataactccc cagccgcgcg tgcataatga taccgcccat gctgtgtccc atatagttaa
       61 ttttgggggc tcccgtttct ttttgcacaa agcggatcgc ggcgtctaaa tcttgggtga
      121 tataatcgtc gatgtcgtac ttatacgtgt gcttgccgaa gaaaaaaccg gtctcacccg
      181 cgtcgttgcg cccgcgtaaa tccatcagcc acacatcgta gccggcgttt tgtagcgcac
      241 gcacgagtga gtcgtcgtct ttgagtttga agtattgccg gttggcggca aggccgtggc
      301 aaattaagat cggatacttg cgtttgactt gcggcgatag cgaaggcaga tgttcaatcg
      361 tgagagccca accgtcgtgc gtcaccaccg tgtttttttc gccttgaacc ggcagcgatt
      421 gacacgccgc gaaaaaaaat aaaacggtga agaggataaa tcttactttg aaaaaaaaat
      481 tacgctgcgc tgttttcata ggtaaaaacc taaacgtaca cggtatgtcg gtgcggataa
      541 atcgagcgcg gcacttttgg cgtattgcca aacgctataa cccgcttcgc cttccaaaaa
      601 gaacacaccc caatagtaaa gcgtcgtccc cacgaagggc gtgatgcggg tcgaccaaga
      661 agcgctgagg gtgttggttg aaaaatgcac aaagcccgat tctgccccca tgtataaatc
      721 aaaccaaaag cgaggaaaaa gatggaacgc accgcccaac gtcatggggt aatactccat
      781 actggaaata tttttggaaa gcgggatgcc cgcagtgccg cgcagaccaa aacggtcgcc
      841 catcatgaac tcaaacgtgc cttgcagcaa gtgcgcacga tatgcggtat gattttcttg
      901 cgaccaaact gagtttcaaa ttcgattgtt ggatgccgtc gtttgagtcg tctcccattt
      961 ggttgggggc aatcgcaaag agggtggtaa acgataacaa acacaacgtt gtgcaaattt
     1021 ttttcatgat ggctatacgc actgtaaaaa gagtgtgtaa agcgtttctg aaatatcttt
     1081 ctgttttttt aataaatttt tcccaaccgc tcgcgttggt aagagccgtc ttgcacgcgg
     1141 cggcaccact ctgcgtttgc taaataccac tctaccgtct ttttaaggcc cgactcaaac
     1201 gtctcttgcg gtttccatcc caactcatgg gcgatttttt gcgcgtcgat agcgtaacga
     1261 cggtcgtgcc ccgggcggtc ttttacatac gcaattaaat ctttgtacga gcgtccatct
     1321 gcacgcgggc gcagcgtgtc taaaagagag caaatggttt caacaaccgt taaatttttt
     1381 ttctcgttat gcccgccgat attgtacgtt gcacccaccg tgccgcgcgt caccaccgtg
     1441 tagagtgcgc gcgcgtggtc ttcgacgaaa agccaatcgc ggatttggtc gcccttgcca
     1501 tagaccggaa gcggttttaa ttccaacgcg ttcaaaatca tgagtgggat taacttctcg
     1561 gggaaatggt atgggccgta gttattagaa caattcgtga cgagagtcgg caaaccataa
     1621 gtgcgccgcc acgcccgcac caaatgatcg gacgacgctt tgctggcgga atagggagaa
     1681 ctgggcgcgt acgccatttt ttcggtaaaa agaaaatttt tgtaatcgga agtttcatcg
     1741 gggtgcggta gatcgccgta cacttcgtcg gtgctgacgt gatggaagat aaagtttttc
     1801 cgtacgcttt cctccgcatt tgcaatatac gcgcgcgccg cctctaaaag cgtgtacgtg
     1861 ccgacgatat tggtttggat gaacgccgcc ggaccgtcga tggatctatc gacgtgcgat
     1921 tcggcggcga ggtgcatcac ccgatcgggt ttatgctcgg cgaaaacacg cgccgtctct
     1981 tttgtgtcgc aaatgtccac gcgctcaaat gcgtagcgcg gattttgact aacgccggca
     2041 agcgactcca aattaccagc ataggtgagc ttgtccacgt tcacgacggc gtcgtcggtg
     2101 tgttggatga tgtgacgcac caccgcagaa cctataaatc ccgcgccgcc ggtaatgagt
     2161 attttcaaga tttcaccaac tctacttctt tttgcaactc ttcaaccaga atttttagtt
     2221 cgtcgtactc gcgcattttg cgttttaaga agccaatcgt taccctcgct ttttgttcta
     2281 ctccgctggg ggtgaggata tacgcgtacg caagtttctt tttggtgctc ttaaagtttt
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaatgca atagttgact
     2401 ttccctaaac tcattcccaa atgttgcgaa agctcacgtt ggttgattgt gggattttct
     2461 tcaatcagtt tgaatagtct gtgttgaatt tcatcgtcca tgcgtattga tgcgccatga
     2521 aattaaaact gctcggcgag tttattaccg agcgtttgta gttgcatctt tgcaccgttg
     2581 cggatgtcgg caggcatttg ttgagcgata cgcacaattt tttcgtggtt catcattgcg
     2641 ttggcaatat tgcctacaat tttaccgtcg cgtaacgcgt tcacaatgtc gagcaacacc
     2701 ttgtcgtcga gtaaatcggc gagctcgccc aatttgccgg taaaattttt ggtcaccagg
     2761 tctttcacca cttgcgcggt gatattgact tccattagat taccgacgcg cgcagtcacc
     2821 tctgggttgg cgtagagagc gacttggccc atgtagtcga tgcgtagcga tttgccaatt
     2881 ttggccgccg ctttgggatc catataaatc accacctgag cgatgatgta cggtgtgaga
     2941 tagtcgtgcg cgagttttgc aatgataaaa ttggggataa actgcgttgc aacggccatc
     3001 actttgaaaa cgggtttttg taattcttcg gtgcgttgta tatacccttg gatttctttg
     3061 tggatttttt cgatggtctt gtcgtcgatt ttttctaaat aacttaagtc gttttcggta
     3121 actaaaattt ctttgatttc agagatgaga attgattttt ccatgcgata cctcttgttg
     3181 gtcagtgagt atatccccag ctaaaaattt aggacagcaa ccgcagccga tgtgaggggt
     3241 agtcgtgatg tcaacgaaag ttttgtcgtc atttgtttgc cgttgaacga ttcaaatttt
     3301 ttcgcaccgc agtcgagagc aagtttacgc atatcgtcga ccgttgcacc atacgtaaat
     3361 ttttcattgt aaattaaatc catcgcgtag gggagtatgg cgtgtaaaat acttttgaac
     3421 ggatggtcga cgagggtttg ccaataatca taaagaagat aactcccctc gccaaaataa
     3481 tcgcgcaccg ctctaatggt ggagcatacc acttctttgg tgacgtacat cgatactccc
     3541 tcccaaagca cgagtgtggg ttttttttcg agtttggcgt gggtaaaaat ttcagcgagt
     3601 gactctttca taaagtcgac ttcgtaatag tgaacctctg cgccgcgtgt tttttcgtta
     3661 cgcagataga attttttacg gcgctgtgtt gcgggaaaat cgaattccca aatcccatgt
     3721 ttaagggagt cgttgaggcg tagcgcacgg gaatcgtatc ctgcacccaa gagcaccacc
     3781 ggcatttggg gggctaaatc ttttacgtat ttatccatga acgcatggcg cactgagata
     3841 aaatcgaaaa gccccaacga cgcggcgttg acaaaaaaag atacaaagtc gggtaaaatc
     3901 gacagtcgat taaagatgcc agtgtaaaac tcggaaagaa accgtaccgc gtatttgtcg
     3961 tgcataatgc tcttttcgcc gtaatacgca ttttcgagag cgcggatgag ggccacaatt
     4021 tcagcggtcc gactcggggt attgtctaac atgggtgcca tttttgtcga taattgatag
     4081 agacaagtac tttatgcagt ttccattttt tcagaaaaaa aagccatccc actcggtaga
     4141 agaccaaacg gaattgctat cgaaactttt gacaaaatat cgcaacgccg aaacaaaata
     4201 cggtaagaag ttttttgata aaaccaaact cgacgaacgc atcatccgat tttgagcgtt
     4261 ttattttgga cgaaatgcaa ttttataaag caatggaaga gaaagccatc gaagaggaag
     4321 cgcgtcgtct gcgccaagaa gaagccgacg cacacatgcg tgcaattatg gctgaaaacg
     4381 acgcgcgtat cgaacattac gaagacacat ttttccaccc gctcgcaacg ctcgaatgcc
     4441 gacgtatcgt gagcgccgtt tcgtcgcttt tccccgaggt ggaagaagaa ctgcgttatg
     4501 tgtatcaagg gcgcaaagag tggcaggaga tgaaatttaa tttgtcggat ttagagcgtt
     4561 tcctttacaa accgccgatg cgcctcacgg cgtttttggc gcactacatc aacgaaatac
     4621 aaaaatgggg cgagggggga cgcgaagaag ccgaccgtaa atttcttcaa acggccgcga
     4681 tgtgtctaag cgccatcagc aatgggataa ctacctcgat agaccatctt actgaaaaag
     4741 acaaagccat cgcagaaaaa attcgcactc gcctcgatac ggttgtcgac gattttcgtt
     4801 tgggggattt agcgcagcat gggctgaaaa gaaaatcggc gtcaggcgac ccgcacaacc
     4861 actttaccaa aatgactacc gctctttaag tagcgcagcg cattgggaag ttcgtcaaaa
     4921 ccaaaaacgt tatcaaccac cggtttcatt tgggtctcac caacggcagt acacatcgct
     4981 tcaaagcctt cgcggccacc gacgagcaca ccctgcatcc gcacgttttg catgaggatg
     5041 gggagtaggt tgagttcttt tgaggaaccc gcaagtacgc cgatgaggct cacaacacca
     5101 tccatccgca ctgcgcgaat cgattgctcc aatgtacccg caccgcccac ttcgacgatg
     5161 tggtctgcgc cgcgcatttg ggtaatctta cgtacttctt ttgcccagtc tggtttttct
     5221 ttatagttaa taaaaaaatc cgcacccaat tcacccgcgc gtttgagctt ctcgttcgaa
     5281 ctcgatgtcg cgattacttt ggctcccatt attttcgcga actgcaacgc aaatatagac
     5341 acaccaccgg tgcccagcac gacgacgtac tcgcctgggt ttatatggtt caaggtcacg
     5401 agcgccgacc atgcggtgag cgccgcgcag gggagtgtcg ccgcttcttc gtaagttaag
     5461 tgctgtggga tttccaccgc agcccattca ggt
//
LOCUS       DAOMPD010000143         5476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6288_length_5476_cov_2.801144, whole genome shotgun sequence.
ACCESSION   DAOMPD010000143 DAOMPD010000000
VERSION     DAOMPD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_5476_cov_2.801144"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLY93_06265"
     CDS             <1..755
                     /locus_tag="PLY93_06265"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRP69117.1"
                     /translation="VWPGNELRVEYHPKNITSHETNALFDGESILYHEDGILIVNKPA
                     GLPTVATIDNARDNLVTQLKVYLGRDMDRPPVESRPGTSMYLGIHHRLDAPTSGLILF
                     TTKEEMNPFAARLFQESLIHKEYLAVCIGGNPTVGKKWEIKNQLARAAKKKNIYHSSQ
                     EGSFAYSRFEVLAIKDNRALVRCVPITGRTHQLRVHLSEYGMPVVGDRTYGGVAAPRL
                     MLHAHRLTFRHPHEAHEVVVEAPVPKEFDFFL"
     gene            822..1178
                     /locus_tag="PLY93_06270"
     CDS             822..1178
                     /locus_tag="PLY93_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRP69118.1"
                     /translation="MGVASLVLGIVSLVFAIIPVFWPIAWVGAICGVIGLVLGLLARK
                     NAKAANQPTGSATAGFVMSIIGTILTALIYIACVAAVSKATKALNDPELQKKFEQGLD
                     KALEEAKKEAEKEADK"
     gene            1233..2030
                     /locus_tag="PLY93_06275"
     CDS             1233..2030
                     /locus_tag="PLY93_06275"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRP69119.1"
                     /translation="MHPILFQIEAAGITRTFTTYGVMAVIGIFTGALLSIRIAKKRGY
                     NAFDFTNIIALLVAGGIVVSLFTHFLIFLPERLAAPSFWEMQWGVVSWGGVVGGFLAA
                     LYASRAWKIPLLRLGDIVIPGVALGFAFGRLGCFFAGCCYGLHYDGLLHLTFTHPLAP
                     AAMEMQPLFPIQPLSAALLFALCLILLWVVKKQFVEGMAVVVYAILYSIGRFTIEFFR
                     NDPRGIYLHLSDAQWYSLFLFTVGVLLWFYLSRRGKKSAPHEENYGH"
     gene            2020..2931
                     /locus_tag="PLY93_06280"
     CDS             2020..2931
                     /locus_tag="PLY93_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP69120.1"
                     /translation="MVIEINEVSRLFGTKRAVDKVSLTINPGERIGILGPNGSGKTTL
                     IQLVLGFLKPNEGSIRIDGLDAWQHRFMNRRRIGALIEIPLLYPSLSARENLLFFARF
                     SAAPLTRIDELLKLVDLDPKETKPFSTFSLGMKQRLGIALALLNDPEFLIFDEPTNGL
                     DPEGIVQVRTILKDITKMGKSLILCSHLLPEVEQICDNVAILQDGKVLAKRNVKELSR
                     EGNSFRITAENIESAEKILNTVGIQSMEKSADNAIRVVLDAAAQPSAFLKSIIESGLK
                     VEEFVRLNAGLENFFMNLIESQKGKEK"
     gene            2928..3746
                     /locus_tag="PLY93_06285"
     CDS             2928..3746
                     /locus_tag="PLY93_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69121.1"
                     /translation="MKSQFISEFTAAFAAEIRKLTKLKTHIAFLAITLPLMLIIMGIE
                     VAMSHNAPGGSAQVITPVNADVTWKIFHFFSQYLTPLIAALVVASQVGKEFEWKTFHQ
                     IQLKGQTPLVYVTSKLTALYTMVTGVYVVQVVVVFLFYIVRALIENQSIDIPVGQLAT
                     DTLFYFTALPIALMLAVLTASASIGIVLSLVHLLVLEMIFFPLVWQLMSALGHPNVST
                     VLSYSPMKLPVQIVEHSEGFLETFALVSAELTFIALMGYLAYIVLNRRQIGLIR"
     gene            complement(3755..5152)
                     /locus_tag="PLY93_06290"
     CDS             complement(3755..5152)
                     /locus_tag="PLY93_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRP69122.1"
                     /translation="MLPTSETPIQNFPESRLARWRTILSIAWPLIVANSFWNIQMTID
                     RLFLADYSTDAMGAAIAVMGVFWVPMALLQQTASYVTTFVAQYFGAKRDAMIGPAVWQ
                     SLYVGLIGGLLFLGFIAFAKPLFTFMGHPASTQQLEVDYFIAMCFSALPTAFVATASG
                     YFTGLGRTRVIMQINGVGLVVNAILDYAMILGKWGFPKMGIAGAGYATAIATAMAALY
                     GFFLMFSDKKRHEYRLTSGYTWDTPLMKRFIRFGVPSGLQWALEGLAFSVFLIFVGRM
                     TGGDAALAASSIVVTIMMLSVLPAMGVAQASAVLLAQFLGEKKSDQAERAVWAGWQVT
                     FIYIACASATFLLFPQFYMNWFKNNDNAMLWAQVSTMVPYLLVFVATFTIADSINLVF
                     SFSLKGAGDTKFVTILALVLPWPLMVIPSFFLINLEGGLYWSWGAATVFSATQATIFW
                     RRFVGGKWKNMSVIG"
     gene            5271..>5476
                     /locus_tag="PLY93_06295"
     CDS             5271..>5476
                     /locus_tag="PLY93_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRP69123.1"
                     /translation="MAKEYQGACLCGAVHYTVKGPFLRFFFCHCSRCRRSTGTAHAAN
                     TFVRAGNVTFTQGENHIKRFDLPDA"
BASE COUNT         1424 a         1365 c         1276 g         1411 t
ORIGIN      
        1 cggtgtggcc ggggaatgaa ctacgcgtcg aataccatcc taaaaacatc acttcccacg
       61 aaacgaatgc acttttcgat ggcgagagta ttctttatca cgaagacggt attctcattg
      121 tgaacaaacc cgcaggattg cccacggtgg cgactataga caacgcgcgg gacaatttgg
      181 tgacgcagtt gaaggtttac cttggtcgtg acatggatcg acctcctgtc gagtcacgac
      241 ccggtacatc catgtacctt ggaatacacc atcgtctcga tgcaccgaca tcgggattga
      301 ttctattcac cactaaagaa gaaatgaatc cgtttgcagc gcggctcttt caagaaagtt
      361 tgatccacaa agaatacctc gccgtctgta tcgggggaaa tcccacggtg ggaaaaaaat
      421 gggaaattaa aaatcagctc gcgcgtgcgg caaagaagaa aaatatatac cattctagtc
      481 aagaaggctc gtttgcgtat agccgctttg aggtactcgc cattaaagac aaccgtgcat
      541 tagtgcgttg cgtgcccatc accgggcgta cgcaccagtt gcgcgtacat ctttcggagt
      601 acggcatgcc cgttgtgggt gacagaacgt atggcggtgt cgctgcgcct cggctcatgc
      661 tacacgcaca ccgcttgacg ttccgccatc cgcacgaagc gcacgaagtg gttgtggaag
      721 caccggtgcc taaagaattt gatttttttc tttaatgtct tgacatctgt gtccactgtg
      781 caaaatagtg cttacaaatt ttttttactc ttggaggtaa aatgggagta gcttcactcg
      841 ttttagggat tgtatccctc gttttcgcga taattccagt attctggcct atcgcatggg
      901 tcggtgccat ctgtggtgtc atcggcttag ttctcggtct tttagcgcgt aaaaacgcta
      961 aagcagcaaa tcaaccaaca ggttctgcaa cggcaggctt cgtaatgagc atcatcggta
     1021 caattttaac cgcgcttatc tacattgcat gcgttgctgc ggtttcaaaa gcaacaaaag
     1081 ctcttaacga cccagaacta caaaaaaaat tcgagcaagg cttagataaa gctctcgaag
     1141 aagccaaaaa agaagcagag aaagaagcgg ataaataacc gctctatcga ttgacttagt
     1201 ttgagccggc cttgcggtcg gcttttttat tcatgcaccc tattctattt caaatcgaag
     1261 ccgctggtat cacgcgtacc ttcaccacct acggcgtgat ggccgtcatc ggtattttta
     1321 caggcgctct cctcagtatc cgcattgcca aaaagcgcgg ctacaacgct tttgatttta
     1381 ccaacatcat tgctctcctc gttgcggggg gtattgttgt ttctttattt actcattttt
     1441 taattttttt acctgaaaga ctcgccgccc cttcgttttg ggagatgcaa tggggcgtgg
     1501 tgagttgggg cggtgttgtc ggcggatttc ttgccgcgct ctatgcctcg cgtgcttgga
     1561 aaatccccct gttgaggctg ggcgacatag ttatccccgg cgtcgcgttg ggctttgcgt
     1621 ttggccgcct cggctgcttt tttgcggggt gttgctacgg tctccattac gatggcctgc
     1681 tgcatctaac cttcacccac ccgttggcgc cggcagcgat ggaaatgcaa ccccttttcc
     1741 cgatacaacc tttgtcggcg gcattacttt ttgcgttgtg tctcatttta ttgtgggtcg
     1801 tcaaaaaaca attcgtggaa ggtatggccg tcgtcgtgta tgcaatactt tacagcattg
     1861 gccgcttcac gattgaattt ttccgcaacg atccacgtgg aatttatctt catctttcgg
     1921 atgcacagtg gtattcgctc tttttgttta cagtaggcgt gcttttgtgg ttttatttat
     1981 cccgtagggg caagaaatca gcaccgcacg aggagaatta tggtcattga aattaacgaa
     2041 gtctcgcgcc tttttggtac gaagcgtgct gtcgacaaag tttcgttaac aatcaaccca
     2101 ggcgagcgca tcggtatttt agggcctaac ggctcgggta aaacaacgct catccagttg
     2161 gtattggggt ttttgaaacc caacgaaggt tccatccgca tcgatggact ggacgcttgg
     2221 caacaccgat tcatgaatcg acggcgcatc ggtgcgctta ttgaaattcc actcctatac
     2281 ccatcacttt cggcgcgcga gaatttactt ttctttgcac gcttttcggc cgcaccgctc
     2341 acgcgcatcg acgagctttt aaaactcgtg gatttggacc ccaaagagac aaaaccattt
     2401 agcacattct cgctcggcat gaaacaacgc ttggggattg cgctcgcact cctcaacgat
     2461 cccgagtttt taatttttga cgaacccacg aacggccttg atcccgaggg gattgttcag
     2521 gtgcgcacca tcctgaaaga catcactaaa atggggaaat cgctcatttt gtgcagccac
     2581 ctcttacccg aggtggaaca aatttgcgac aacgtggcga ttttacaaga cggtaaagtg
     2641 cttgcgaaac gcaatgtgaa agagctttcg cgcgaaggga attcattccg catcaccgca
     2701 gagaacatcg aaagcgccga gaaaatttta aataccgtgg gtattcaatc gatggagaaa
     2761 tcggcagaca acgccatccg tgtcgtttta gacgccgctg cccaaccatc cgcttttctc
     2821 aaaagcatca ttgaaagtgg actcaaggtt gaagagtttg tgcgcctcaa cgcaggtttg
     2881 gaaaactttt ttatgaacct tatcgaatcg caaaaaggaa aagaaaaatg aaatcgcaat
     2941 ttatatctga gttcaccgca gcgtttgccg ctgaaatccg taagctcaca aagctgaaaa
     3001 cacacattgc atttcttgcg ataacgctac ccctcatgtt aatcatcatg ggtatcgaag
     3061 tagccatgag ccacaacgct cccggcggca gcgcccaagt gattacaccc gtgaacgccg
     3121 atgttacatg gaagattttt cattttttct cgcagtattt aacccccctc attgcggcgc
     3181 tcgtcgtggc gtcgcaagtg ggtaaagaat ttgagtggaa gacgttccat caaattcaac
     3241 tcaaaggtca aacgccgctg gtatatgtaa cctcaaagct cacggcgttg tacactatgg
     3301 tgacgggtgt ctatgtcgtg caagttgttg tcgtttttct tttctacatc gtacgcgctc
     3361 taatcgaaaa ccaaagcatc gacatacccg tggggcaact ggccaccgac acattatttt
     3421 atttcaccgc actgccgatt gcgctcatgc tggcagtgct gaccgcctcg gcgtccattg
     3481 ggattgtttt aagtctggtt catttacttg tgcttgagat gattttcttt cctcttgtat
     3541 ggcaactcat gagcgcattg gggcatccca acgtttcaac cgtgctaagt tactcgccga
     3601 tgaaactccc cgtacaaatt gtcgaacaca gcgaaggatt ccttgaaact tttgcactcg
     3661 tgtcggcaga actcacgttt atagctctca tgggttactt agcgtatata gtactcaacc
     3721 gccggcaaat tgggttgatt cgatagttta cacactaccc aatcacgctc atatttttcc
     3781 atttaccacc gacaaatcgg cgccagaaaa tcgttgcttg agtggcgcta aatactgtcg
     3841 ccgcacccca cgaccaatat agtccccctt caaggttaat caaaaaaaag ctggggatga
     3901 ccataagagg ccaaggcaaa actaacgcca aaatggtaac aaactttgtg tcccccgccc
     3961 ccttcagtga aaatgaaaaa acaagattga tgctgtctgc aatagtaaag gtggcaacaa
     4021 acaccaaaag atagggcacc atcgtgctca cctgcgccca gagcatagcg ttatcgttgt
     4081 tcttaaacca attcatataa aactgtggaa agagtaaaaa agtagcgctg gcgcacgcaa
     4141 tataaatgaa cgttacttgc caccctgccc atactgcgcg ttctgcctgg tcgctttttt
     4201 tctctcccaa aaattgcgca aggagcaccg cgctcgcttg ggcgacaccc attgcaggta
     4261 acaccgacag catcatgatt gtcaccacaa tcgacgaggc ggcaagagcg gcgtcgccgc
     4321 cagtcatgcg accgacaaaa attaaaaaca cgctaaaggc aagcccttcg agcgcccact
     4381 gcaaacccga tggtacgcca aaacgaataa aacgtttcat gagaggggtg tcccatgtgt
     4441 acccggatgt caaacggtac tcatggcgtt tcttgtcgct aaacatgagg aaaaaaccgt
     4501 agagcgccgc cattgccgtt gcaatcgccg tcgcgtaacc tgcaccggca attcccattt
     4561 tgggaaagcc ccacttgcct aaaatcatag cgtagtcgag tatcgcatta acaacaagac
     4621 ccacgccgtt tatttgcata atcacccgtg tgcgtcccaa acccgtaaaa tagccgcttg
     4681 ccgtcgctac aaacgccgta ggcagcgctg agaaacacat ggcgataaaa taatccactt
     4741 cgagttgttg cgtcgaagcg ggatgtccca taaaagtgaa gagaggtttt gcaaaagcga
     4801 taaaacctaa aaaaagtaat cctccgatga gaccaacgta gagagattgc catactgctg
     4861 ggcctatcat agcgtcgcgc tttgctccga aatactgtgc aacgaaagtg gtcacataag
     4921 atgcggtttg ttgcaatagc gccatcggca cccaaaaaac tcccatgact gcaatcgccg
     4981 cgcccattgc gtcggtcgaa tagtctgcca aaaaaagacg atcgatagtc atttgtatat
     5041 tccaaaaact attggcgaca ataagcggcc acgcgataga aagaatcgta cgccaacgcg
     5101 ccaagcgact ttcgggaaaa ttttgtatgg gtgtttctga agtgggaagc atagttatgt
     5161 agctatttcg agaaagtacg gtattgaagt ctgaatgggg tgtaaatcga atagctttgt
     5221 gagttcgact tatgtcttta cgagaggcct tactctgatt ttatgaacgt atggctaaag
     5281 aatatcaagg cgcttgcctt tgcggtgcag ttcattatac ggtgaaagga ccgtttttaa
     5341 gattcttttt ctgccactgt tcgcgttgcc gccgctccac tggtacggcg cacgcagcaa
     5401 atacattcgt gcgtgcaggc aacgtgactt tcacccaagg agaaaatcac atcaaacgat
     5461 ttgatttacc cgacgc
//
LOCUS       DAOMPD010000144         5476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6290_length_5476_cov_2.589006, whole genome shotgun sequence.
ACCESSION   DAOMPD010000144 DAOMPD010000000
VERSION     DAOMPD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6290_length_5476_cov_2.589006"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1739
                     /locus_tag="PLY93_06300"
     CDS             249..1739
                     /locus_tag="PLY93_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69124.1"
                     /translation="MRCSQRAWHFTTTQGLPLEERLWRISDTLLYESGYAQAQLLADE
                     WTSYTAKLTQGTLHAPALDVLVNEPAILGLLQNPKGPLAPVFEQESAALLDNFANGAL
                     KAWRLRSIDQKIVLARDQNRLAQIDIGVIRREVGIRYFSIPIQSMGNNYVLDGEWDIT
                     RFADFPLSQARSTYMFLTTDKELNILSLGNTAPTYTTNPDWIRGSKFNAPLFGIHKGQ
                     RIFSVQGKDFNLIIVYPFAGYAFYAIRGAILLLVIVALLIFLVKLATIRLSAKHVLEN
                     RQKNWLNQHYEESLAMNAEALQLGDKALGVVSSLKEREARIIGEFGRHLHELSNNFTA
                     ESRLLIEEAQKTQSIQNAQPLSKHTENAQVSTEKKAPLHKKAVYKKPILINAKEGSEI
                     EVSIELDLPLTNEKELSPHTKATYIGSLRRRAQEKAGIKNDYLRDETVENYSYPPEPP
                     LPMPQNNEKELPRSNKDVDLSSIQNFRYTGKKHALPLVVPEDELTK"
     gene            1765..2196
                     /locus_tag="PLY93_06305"
     CDS             1765..2196
                     /locus_tag="PLY93_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69125.1"
                     /translation="MRKLFVWSQALLLTSYFAVSTNVAAASKCQGLSQSQCQSDDDCT
                     WVGAYSQKDGDKVNAYCRAKPGKGDSSKSNKKKNKKEKATETVNADDSASTNSTEKKE
                     KKKKKKNKKEKAEKDSDKDTKETKKDKKEKKSKKKKSKKDK"
     gene            complement(2240..2632)
                     /locus_tag="PLY93_06310"
     CDS             complement(2240..2632)
                     /locus_tag="PLY93_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69126.1"
                     /translation="MQVNKTEKKWSVVIEDQDNKLGLINMIRHLDRVVGELEMVKIPN
                     LILEIDLAKLNSANSELIAKFVELQSILVRTDGRLNVINANPELKSSFDVVMLDKIIP
                     IQYMGQEDPGEDGYAYDDYELPDDGSDE"
     gene            2722..3234
                     /locus_tag="PLY93_06315"
     CDS             2722..3234
                     /locus_tag="PLY93_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69127.1"
                     /translation="MAKSETTKQKKESACSFRSQIDYITVGLPFAAFKILFALALLRA
                     QSGLFATSVAYALIALGVIDLTLNLLNSLSCLLLKKSITPVCFFASALALFSRKRSYD
                     APQHARHIDLGMALDVMLSFILVALCLALQYLKYFNSTELWTWNVAVIFNVLGAGIQR
                     LLTSLQKEDR"
     gene            3237..4013
                     /locus_tag="PLY93_06320"
     CDS             3237..4013
                     /locus_tag="PLY93_06320"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRP69128.1"
                     /translation="MPQVHIERAAQILRNAKHPFFLSGAGISAESGVPPFRGPADSHG
                     IAAVSGPEREIPLWSKFRAEDLATPEAFQRDPTMVWDWYNWRKRLIREKEPNAAHFAV
                     RTMAEKLPQLCGVTQNVDTLHQRAGLQSIYEMHGSIFHTRCTACGDTRENKDDVTPET
                     PCTVCAKTALRPHIVWFGEALDAALMEKIYSALSRSDAILVVGTSGHVYPAAGFAIEV
                     RRRGGCVIEINTDANHRAFANDIYIQGKAAEILPQLVALI"
     gene            complement(4005..5144)
                     /locus_tag="PLY93_06325"
     CDS             complement(4005..5144)
                     /locus_tag="PLY93_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP69129.1"
                     /translation="MRDYLTSRIHGIDTSEIRKVFDLAAKIQNPLNLSIGQPDFPVPQ
                     PVKEAMEKAIQDNCTAYTPTAGLVALREAIGTFLQPKIGYKTLSDNIIVSTGVASILF
                     LLFQTIIEKGDAVLMVDPYFMIYQALIRYHEGKEYTIPEHFGQSEVDALRARLKKEGV
                     RLKLIIFASPSNPTGKILNREQLELLKTLAEENDAVIASDEIYAAFDYEHKHTSMATL
                     APEHTLTLNGFSKSHAMTGLRVGYLAAPERLKTIVQKMVTLQQYTVVCAPHVMQVAAI
                     TALKTPIDAELKFMRKRRDLVYGILSQATTLPYPDGAFYVYPDIPIDSADFVTKAIER
                     RLLLVPGHIFSANRKSIRISYAVKEEILEEGARIFSKLVEELKSN"
     gene            complement(5154..5393)
                     /locus_tag="PLY93_06330"
     CDS             complement(5154..5393)
                     /locus_tag="PLY93_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69130.1"
                     /translation="MLTRVLFIFCVVTVSVTFLACGESYCKQYAAKFCTDPNTPACKT
                     AQEKIKNWSSNECRIQFNNLIIEEQAKDTEKLLEE"
BASE COUNT         1553 a         1334 c         1216 g         1373 t
ORIGIN      
        1 taagaggagc ctgtgcgcca actcgcgcag cgggtacaaa gagagaattt ttttgcaaat
       61 ttttgtacgc ggttcgctca aaaatacaaa atagcctaca aaaatttgca aaaaaaattg
      121 tctcagcgag ttggcgcaca ggctcttaag attaccccct caaaaagtcg ataataaaga
      181 gtcgcctcat gtccaaaaga cgcatcattc tttttttact ttttcttcct ttattagtcg
      241 tacttgctat gcgctgctcg caacgagctt ggcatttcac cacaactcaa gggctacctc
      301 ttgaagaaag gctttggcgc atcagcgaca ctctgctcta cgaatccggg tatgcgcaag
      361 cacaattatt ggccgacgag tggacatctt atacagcaaa gctaacgcaa ggtacactcc
      421 atgcccctgc tctcgatgta ttagtgaatg aaccggcgat tcttggtctt ttacagaatc
      481 ccaaggggcc actggctccc gtctttgagc aagagagcgc cgctttattg gataactttg
      541 caaatggtgc gttgaaagcg tggcgtttgc gcagcatcga tcaaaaaatt gtgcttgcac
      601 gcgatcaaaa ccggttggct caaattgaca tcggcgtaat ccgtcgagaa gtcggcatcc
      661 gctatttttc aataccaatc caaagcatgg ggaataacta tgttctcgac ggtgaatggg
      721 acatcactcg tttcgcggat ttccctttga gtcaagcgcg ttcaacgtac atgtttttaa
      781 ccaccgacaa agaattgaat atactttcgc tcggtaacac cgctcctacc tacacaacaa
      841 accccgactg gattcgtggt tccaaattta acgccccact ttttggtatt cataaaggac
      901 aacggatatt ctcagtacaa ggaaaggact tcaaccttat tatcgtctat ccgtttgccg
      961 gctatgcttt ttatgcaatc cgtggagcaa tcctcctgtt agtcattgtc gctcttttga
     1021 ttttcctcgt taagcttgcg acaattcgtc tttccgcaaa acacgttctg gaaaatcgcc
     1081 aaaaaaattg gctcaatcaa cattatgaag aaagcctcgc catgaacgcc gaagctctcc
     1141 aactggggga caaagcgctt ggagtggttt cctcgctcaa agaacgtgag gcgcgcatca
     1201 tcggcgaatt tggccgccat ttacacgaac tcagcaataa ttttaccgcc gagtcgcggt
     1261 tactcatcga agaagcgcaa aagacacaat cgatacaaaa tgcccaaccg ctttcaaagc
     1321 atactgagaa cgcgcaagtg tccacagaga aaaaagcgcc gctccataaa aaagcggtct
     1381 acaaaaagcc gatattgatt aatgccaaag aaggcagtga aattgaagtt tctatcgagt
     1441 tggatttacc tcttaccaat gaaaaggaac tctcgccgca cacaaaagcg acgtacatcg
     1501 gttctttgcg ccgccgtgca caagaaaagg caggcataaa aaacgattat ctgcgcgacg
     1561 aaaccgttga aaattattcg tacccacccg aaccaccact acctatgcca caaaataacg
     1621 aaaaagaatt accgcgcagc aataaagacg tagatttaag ctcaatccaa aactttcggt
     1681 atacaggcaa gaagcatgct ctacctctcg tggttcctga agatgagttg acaaagtgag
     1741 aaaatcccat ttcaccgctc cactatgaga aaattatttg tatggagcca agcgcttctg
     1801 ctaaccagtt attttgcagt gtctaccaat gttgccgctg catctaaatg ccaaggctta
     1861 agccaaagcc aatgccaaag cgacgacgat tgcacctggg ttggtgcgta ttcacaaaag
     1921 gacggcgata aagtgaacgc ttactgccgc gccaaacccg gcaaaggcga cagctcaaaa
     1981 agcaataaaa agaaaaacaa aaaagagaaa gctaccgaga cagtgaacgc agacgatagc
     2041 gcctctacaa attcaacaga gaaaaaagaa aaaaagaaga aaaagaaaaa caagaaagaa
     2101 aaagctgaaa aagactcgga taaagacaca aaagaaacta aaaaagacaa gaaagaaaag
     2161 aaatctaaaa agaaaaaatc aaagaaagat aagtaaatat agtttatctt gcgcgcattg
     2221 cgcgcaagat aaacccatcc tactcatcgc tcccgtcgtc gggtagctcg taatcgtcgt
     2281 acgcgtaccc atcttccccc ggatcctctt gccccatata ctgaatggga ataatcttat
     2341 cgagcatcac cacatcaaac gagctcttga gttcaggatt cgcgttgatc acgttcaacc
     2401 taccgtcggt gcgcaccaaa atactttgta gctcgacaaa cttggcaatc agttctgaat
     2461 ttgccgaatt gagtttcgcc aaatcaatct ctaaaattaa attgggaatt tttaccattt
     2521 ccagctcgcc caccactcga tctaagtggc ggatcatatt gatgagcccg agcttattgt
     2581 cttggtcttc gatgacgacc gaccatttct tttctgtttt gttgacttgc ataaattaag
     2641 aagcggcata gatttttttt tacctgtcgc gtcgagtaaa tcacgattta cggcgtctca
     2701 aattttgaaa atgggtgcac cgtggctaaa tcagaaacga cgaagcaaaa aaaagagagc
     2761 gcgtgttctt tccgctcaca gatcgactat attaccgttg gtcttccatt tgccgctttc
     2821 aaaattcttt ttgcattggc gcttctgcgt gcgcaatccg gtttattcgc aacatcggtt
     2881 gcgtacgcgc tcatcgcctt aggggttatc gatctaacgc tcaatctcct caactctctt
     2941 tcgtgtcttt tgttaaaaaa gtcaatcacc cccgtttgct tttttgcaag cgcgcttgca
     3001 ctttttagcc gtaaaagaag ctacgacgca ccacaacacg cgcggcatat agacctcggc
     3061 atggcgctcg atgtcatgct atcgtttatt cttgtggcgc tctgtttggc gctgcaatac
     3121 ctcaaatact ttaactccac agaactttgg acatggaacg ttgccgttat ttttaacgtt
     3181 ttaggcgcgg gcatccaacg tcttttaact tcgcttcaaa aagaggatag gtagcgatgc
     3241 cgcaagtcca catcgagcgc gccgctcaaa ttttgcgtaa cgccaagcat ccgttttttt
     3301 taagcggcgc gggtatatcg gcagaaagcg gagtgccccc atttagaggt cccgccgatt
     3361 cccatggaat cgcggcggta agcggcccag aaagagaaat ccccctttgg tcaaaatttc
     3421 gcgccgaaga tttagccaca cccgaagcat tccagcgcga ccccacaatg gtttgggatt
     3481 ggtacaactg gcgcaaacga ctcatccgcg aaaaagaacc caacgctgcg cattttgccg
     3541 tccgcaccat ggcagaaaaa ctaccgcaac tgtgcggcgt cacccaaaac gtcgacacct
     3601 tacaccaacg ggcgggactc caaagtattt acgaaatgca tggcagcatt ttccataccc
     3661 gctgcaccgc atgcggcgac acccgtgaaa ataaagacga cgtcaccccc gaaacgccgt
     3721 gcacggtatg tgcaaaaacg gcgctgcgcc cccacatcgt ctggtttggt gaagcgttag
     3781 acgctgcgct tatggaaaaa atttacagcg cactctcacg aagcgacgct attttagtgg
     3841 tgggtacctc tggacacgtg taccccgctg ccggttttgc gattgaagtg agacggcgcg
     3901 gtgggtgtgt cattgaaatc aacaccgacg caaaccaccg tgcctttgca aacgatattt
     3961 atatccaagg caaagcggca gaaattttac cgcaacttgt cgctttaatt tgactttaac
     4021 tcttcgacaa gcttgctgaa aatgcgcgcg ccttcttcta aaatctcttc tttgactgca
     4081 taactaatgc ggatggattt acggtttgcg ctgaagatat gccccggcac gagtaaaagc
     4141 cgccgctcaa tcgctttggt tacaaaatct gccgaatcga tggggatgtc gggatagaca
     4201 taaaacgcgc cgtcggggta cgggagcgtt gtcgcttggg agagtatacc gtacaccaag
     4261 tcgcgccgtt tgcgcatgaa tttaagctca gcgtcgatgg gtgtttttag cgcggtgata
     4321 gcagccacct gcatcacgtg cggcgcacac accaccgtgt attgttgtag cgtcaccatt
     4381 ttttgcacga ttgttttcaa ccgttccgga gcggcaagat agcccacacg caaccccgtc
     4441 attgcatggc ttttagaaaa accgttgagt gtcagggtgt gctcgggggc aagcgtcgcc
     4501 atgctggtat gcttatgctc gtagtcaaac gccgcgtaaa tttcatcgga ggcaatgacg
     4561 gcgtcgtttt cttcagcgag agttttgaga agttctaact gttcgcggtt taaaatttta
     4621 ccggtggggt ttgacggcga agcaaagata atgagcttca accgaacgcc ctcttttttg
     4681 agacgagcgc gtagcgcatc gacttcgctt tgaccaaaat gttctggaat ggtatactct
     4741 ttcccttcat ggtatcggat gagcgcttgg taaatcataa aatagggatc aaccatgagc
     4801 accgcatcgc ctttttcaat aatcgtttga aaaagaagga ataaaatcga agcaaccccc
     4861 gttgagacga tgatgttgtc gctcaaagtt ttatagccga ttttaggttg taaaaacgta
     4921 ccgattgctt cgcgcagcgc cacgagcccc gctgtgggtg tgtacgcagt acagttgtct
     4981 tggatggctt tttccatcgc ctctttcaca ggttgaggca cggggaaatc gggttggccg
     5041 atagagaggt tgagtggatt ttggattttt gccgctaaat caaaaacttt acggatttca
     5101 ctggtgtcga tgccgtggat gcggctcgtc aaataatcgc gcatggacag tcgttactct
     5161 tcgaggagtt tttcagtgtc ttttgcctgt tcctcaataa ttaaattatt aaattgaatg
     5221 cggcattcat tcgacgacca attttttatt ttctcttgcg ccgttttaca cgcaggcgta
     5281 ttgggatcgg tacaaaattt agcggcatat tgcttacaat acgactcgcc acaagcaaga
     5341 aaagttacac ttacggtaac aacacaaaaa ataaaaagca ctctggttag cataatataa
     5401 aaagagaaag agaaattagt gcgtcaagga atatacatac cgcagctatg caacccacag
     5461 agataactga cacttt
//
LOCUS       DAOMPD010000145         5473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6293_length_5473_cov_3.046881, whole genome shotgun sequence.
ACCESSION   DAOMPD010000145 DAOMPD010000000
VERSION     DAOMPD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_5473_cov_3.046881"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..382
                     /locus_tag="PLY93_06335"
     CDS             140..382
                     /locus_tag="PLY93_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69131.1"
                     /translation="MGAGSVFAGILRSPEELIEAFFNAWRRLTDAREPMFVLGAFAPS
                     VANALVAIIMVFIFSEHWVNLADNLTTNRNPLPVTY"
     gene            519..1382
                     /locus_tag="PLY93_06340"
     CDS             519..1382
                     /locus_tag="PLY93_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRP69132.1"
                     /translation="MKVFVTGASGNVGIAVIHALIHANVKCEISAGVRSKESPPLALS
                     RPGITIVDFDFTNSSTFAEALRGVEILFLLRPPPISDAKGVFGPLIKAAQEKQVKHII
                     FLSVQGAENSRLIPHHKIEEMIRRSGIAYTFIRPAYFMQNFLTSLNADLKKDHLIYLP
                     AGKAQFTLVDVRDIGAVVAQVIDAYKQYENRALELTSHDLLTFGEMAEQLSQGLGKTI
                     SFVSPSLFSFYRKKRAEGAPRMLILVMIFLHFLPRFSKIPRITDDVELVLKRKPISFA
                     EFIHDYQDSLR"
     assembly_gap    1796..1825
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1826..2073
                     /locus_tag="PLY93_06345"
     CDS             <1826..2073
                     /locus_tag="PLY93_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69133.1"
                     /translation="YSYYRKSVSAGGISDSYATEDNANVGHAGDLALVYNVKEDVQFF
                     YGFGLFKPGAAYPVLKFDGTDGQAWRYAHLVSLTLIL"
     gene            complement(2226..2819)
                     /gene="metW"
                     /locus_tag="PLY93_06350"
     CDS             complement(2226..2819)
                     /gene="metW"
                     /locus_tag="PLY93_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRP69134.1"
                     /translation="MTLDTTRIDYRIISSFVEPKSRVLDMGCGDGELLYLMNKYLQTR
                     SQGIEIDQDQILECIERGINVLLGDLDSGLTDFKDASFDYVILNNALQELRYPDDVIK
                     DALRVGRRLIVGFPNFAYIKARVQIFFHGKLPVPPSLPFAWYESPNLHLLTIRDFKEY
                     CRSRGYKILAERYVRNEKEITLRPNLLAQWGTFLIGK"
     gene            complement(2829..3959)
                     /locus_tag="PLY93_06355"
     CDS             complement(2829..3959)
                     /locus_tag="PLY93_06355"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRP69135.1"
                     /translation="MNATLSDTEKIIVSPQTFSFQNFVCENGEILPQVDIAFETYGRL
                     NENKSNAILITHAFSGDAHAAFYHEGESRAGWWGNMIGAGKAFDTDRYFIVCSNVLGG
                     CAGSTGPSSINPKTGKPYALDFPQVTIRDIVAAQKKLIEHLGIEKLYSIAGGSMGGMQ
                     VLEWLVSYPHAVLSAIPIATTARHSPQQIAFNEVGRQAIMADPKWNGGNYYEGDIPRS
                     GLSVARMVGHITYMSEKSMAEKFGRRTKSAQDSTKFHADFEVEGYLRNRGDFFVRRFD
                     ANSYLYISKAMDLFDASEGRHLSEKFYELEAKLLVLAFKSDWLYPAHQSLDIVTACKI
                     TGVPVSYVEIEANYGHDSFLVETEKQSQLVRHFLSRVDELIY"
     gene            4085..4534
                     /locus_tag="PLY93_06360"
     CDS             4085..4534
                     /locus_tag="PLY93_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP69136.1"
                     /translation="MTIEIHRLHERDFDTWLSLWKEYLLFYKSNVNESTTMLTWKKLL
                     DEKMPMFAWGAFNDRKLVAFAHAVYHPSTWTVGDYCYLQDLFTHPQVRRQGIATKLIH
                     AIYDDAQRHCASRVYWLTHETNVEAKKLYEKIAVRSGFIQYRKVLSL"
     gene            4648..5073
                     /locus_tag="PLY93_06365"
     CDS             4648..5073
                     /locus_tag="PLY93_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69137.1"
                     /translation="MLKTSISLPQKSIPTWAKNHRAIMRIYSRFLRVVVRKTKFRRGV
                     KRLYNKTNGRCAIMKVKFTPHQYDSLHTVAAGIRVSVSFLIFYLIQFWEKASQKNTYF
                     LVADNYAPQVLEWSKTRVPFQEKITFRRVGRRFFTPLRP"
     gene            5151..>5473
                     /locus_tag="PLY93_06370"
     CDS             5151..>5473
                     /locus_tag="PLY93_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69138.1"
                     /translation="MRTTYRYFLIFTFLVLGCTQANVHFPIQKGVLDLRSATIDNSFR
                     INLDGDWEFYWQQLLTPNTFQKPHEQQFIHVPSHWSAVTLDKKNLTGMGFGTYRLNIL
                     LPPKYP"
BASE COUNT         1453 a         1263 c         1254 g         1473 t
ORIGIN      
        1 aaaacgcact caacttgacc ccaaaagtgt cagttatctc tgtgggttgc atagcaacaa
       61 atgtaactga gattgacagt gtcgtgcttc tataaaatat cgcggatgcg cgctactttc
      121 ttttgcactt gccggtttcg tgggggctgg cagcgtgttc gccggtatct tacgatcacc
      181 cgaagagctg atcgaagcat tttttaacgc atggcggcgt ctcaccgacg cgcgcgagcc
      241 gatgtttgtt ttgggggcgt ttgcacccag cgtcgccaac gcgctcgttg cgataatcat
      301 ggtattcatt ttttcggagc attgggtaaa tctggcagat aaccttacga caaatcgaaa
      361 tccgctacct gtgacatact agcctttatg gtagttgcag tcttaaaggg ctatatttaa
      421 attgaacaca gcacgcaccc cgcggaaggt aaagcccaaa attgagctat tattgagatg
      481 gatttacatc tgtgctcatt atttgtcagc gctcaatcgt gaaagttttt gtaaccggtg
      541 cttcgggaaa tgttggtatt gccgtaattc atgcgctgat tcatgcgaac gtgaagtgtg
      601 aaatttctgc tggcgtcagg tcgaaagaat cgccgcctct cgcattgagt aggccaggga
      661 ttacgatcgt tgatttcgat ttcacgaata gttcaacatt tgccgaagcc ttgcgcggtg
      721 tggagatctt gtttttactg aggccaccgc cgatttcaga cgccaaaggt gtctttggcc
      781 cgttgattaa agctgcccaa gagaaacagg tcaagcacat catattcttg tcggttcaag
      841 gtgcggagaa cagccgatta ataccgcatc acaagataga agaaatgatt cgcagaagtg
      901 gcatcgcgta tacgttcatc cgccctgcgt acttcatgca gaattttctg accagtttga
      961 atgctgacct caagaaagat catttgatct atttaccggc cggtaaagcc cagtttacgc
     1021 ttgtagacgt acgcgatata ggtgcggttg tggctcaagt catagatgcg tacaaacaat
     1081 atgagaatcg ggcattagaa ctgacgagcc acgacctgct cacgtttggc gaaatggcgg
     1141 agcaattatc ccaggggttg ggaaaaacga tttctttcgt atcgccatcg ctattcagtt
     1201 tttacagaaa aaagcgagct gaaggcgcac cccgcatgct gattcttgtc atgatctttc
     1261 tgcattttct gccacgattt tctaaaatcc cgcgcataac cgatgatgtt gaattggtat
     1321 taaaaagaaa accgatttcg tttgccgaat ttatccatga ctaccaagat tctctacggt
     1381 agcatacatc cagcgcccgc aaagtggcgg atggctaact gaaaacgcgg cgctacaatg
     1441 ttttagaacg cgggatttcc aagacgacaa gccacgcttc gttgaacttt gctatcgtta
     1501 tttcattcgc gtatgtacgc gtatatggcc cttgtaagat cgcgtaccaa agtccaatca
     1561 ccgccccgaa tagttatctg aaatgatatg cagttccatt ttgcgtggtg tattggcgtc
     1621 gatatgccgc aactcgctgg tgccgatgga cgcattcttt cgatgcacgg caaagtgcag
     1681 atattcgaaa acggtcgttg ggccaaagtg gtgcaggtct atcgtctcgg cacgtacttt
     1741 cggccgttca agtcgattta ttcgctgcgc aacctgttgg tgcagctcaa atattnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnaatat tcttattacc gcaagagcgt ttctgccggc
     1861 ggcatttccg attcgtatgc gaccgaagat aatgcgaacg tgggtcatgc gggcgacctc
     1921 gcgctggtgt acaacgtcaa agaggatgtc caattcttct atggctttgg tctctttaag
     1981 ccgggtgccg cgtatcccgt gctgaaattc gacggcaccg acgggcaggc atggcgctat
     2041 gcgcacctcg tgagcttgac gctgatactc taggaaattc caggcgttta gaccccgctc
     2101 aatgaccgga attttcggga atgcaggaat tcacggtcac tgcgaccgtc ttcgatatct
     2161 cgcttcgaga gcggagtcta agcgacctta aactttcaaa ctcacgccac tgaaaccata
     2221 acgagttact tccctattaa gaaagtaccc cactgcgcca aaaggttggg acgcagggtg
     2281 atttcctttt cgttacgcac gtaacgttcg gccaaaattt tatagccacg gctacggcag
     2341 tactctttga aatcgcgtat ggtgagaagg tggaggttgg ggctctcgta ccaagcaaac
     2401 ggcaaagaag ggggcacagg cagcttgcca tgaaaaaaaa tttgcacgcg cgctttaatg
     2461 taggcgaaat tgggaaatcc aacaatgagc cggcggccca cacgcagtgc gtctttgatc
     2521 acgtcgtcgg ggtaacgcaa ctcttgtaag gcgttgttca aaatgacgta atcgaacgaa
     2581 gcgtctttga aatcggtgag gccgctatca agatctccca acaatacatt gatgccgcgc
     2641 tcgatgcact ctaaaatttg gtcttggtcg atttcaatcc cctgcgagcg tgtttgcaga
     2701 tacttgttca tgagatataa caattcaccg tcaccgcaac ccatgtcgag cacacggctt
     2761 ttgggttcaa caaagctgga gatgatacga tagtcgatgc gggtggtgtc gagtgtcata
     2821 gtactttttt aataaatgag ttcatccaca cgggaaagaa aatgccgcac cagttgcgat
     2881 tgtttctcgg tttccaccaa aaatgaatca tggccatagt tcgcttcaat ctccacgtac
     2941 gagacaggca caccggtaat tttacacgcg gtgacgatgt cgagcgattg gtgtgcaggg
     3001 tagagccagt cggacttaaa cgcgagcacc aaaagtttgg cttcgagttc ataaaatttt
     3061 tcactcaaat gcctaccctc cgaagcatcg aataaatcca tcgctttgct aatataaaga
     3121 tacgaattgg catcgaaacg acggacaaaa aaatcgcctc ggttgcgtaa atagccttcg
     3181 acttcaaagt ctgcatggaa cttggtagaa tcttgtgcgc tttttgtgcg tcggccaaac
     3241 ttttccgcca tcgacttttc gctcatgtac gtgatatgtc ccaccatccg cgcgaccgac
     3301 aaaccagaac gggggatatc cccttcgtaa tagttgcccc cattccattt ggggtctgcc
     3361 atgattgctt gccgccccac ttcgttgaat gcgatttgtt gtggcgaatg acgcgcggtc
     3421 gtggcaatgg ggattgccga caaaaccgcg tgcggataac tcaccaacca ttctaaaacc
     3481 tgcataccgc ccatcgagcc gcctgcgatc gaatacaatt tttcaatgcc caagtgttcg
     3541 atgagttttt tttgtgcagc gactatatcg cggatggtga cttgtggaaa atcaagtgca
     3601 tacggtttgc cggttttggg gttaatcgac gatgggcctg tggaacccgc gcatccgccc
     3661 agtacattcg agcacacgat aaaatagcga tcggtatcga atgccttgcc tgcaccaatc
     3721 atattacccc accaacccgc gcggctttca ccttcgtgat aaaacgccgc gtgcgcatcg
     3781 cccgaaaatg cgtgtgtgat taaaatcgca tttgatttat tttcgtttaa gcggccatac
     3841 gtttcaaacg caatatccac ttgtggtagg atttctccat tttcgcaaac aaaattttga
     3901 aaagaaaaag tttgtggaga aacaataatt ttttcagtat cagataaagt ggcgttcacg
     3961 ctatttcatt gactaaagtt ttgtacaggc tgtcattcaa gttttcggtt tgtgtaagag
     4021 tttaaatttg ttaatctctg tttagcgctt gacgaagtta ttctttttca gtcttataaa
     4081 gataatgacg atagaaatcc accggttaca cgagcgtgat tttgacactt ggctctcttt
     4141 atggaaagag tatttattgt tctataaaag taacgtcaat gaatcgacta cgatgctaac
     4201 gtggaaaaaa ttgctggatg aaaaaatgcc catgtttgcg tggggcgctt ttaacgacag
     4261 gaaactcgtt gcgtttgcgc acgccgttta tcacccttca acatggacag tgggcgacta
     4321 ttgctatttg caagacttat tcacccatcc ccaagtacgc cgccaaggga ttgcaacaaa
     4381 actgattcac gcaatttatg acgacgcgca aagacattgc gcatcacgag tttactggct
     4441 aacacacgaa acaaatgttg aagcaaaaaa actctatgaa aaaattgcag tccggtctgg
     4501 attcattcaa tatcgcaaag tactctcttt atagaaactt gtgaaattat ccaaaagtgt
     4561 aattttttca aaaaaattgc actttttttc taaaaaatga gaaaaatcat gcattttttg
     4621 gtatttaata tatagagggc ggcaaatatg ttaaaaacat cgattagttt accacaaaaa
     4681 tccatcccca cttgggctaa aaatcacagg gcaattatgc ggatatatag ccgctttttg
     4741 cgggttgttg tgcgcaagac aaaatttcgt cgtggagtga aacgattgta taataaaacc
     4801 aacggtaggt gcgcgattat gaaagtgaag tttacgccac accagtacga ttcactacac
     4861 acggtggctg cggggatacg cgtgagtgtg tcgtttctca ttttttacct catccaattc
     4921 tgggaaaagg cgtcacaaaa aaacacctat ttcctcgtgg cggataacta tgcgccacaa
     4981 gtgctggagt ggtcaaaaac aagggtacca ttccaggaaa agataacttt caggcgggtt
     5041 ggacgccgtt tcttcacccc actaagacca tagtgcaatc ccgcgcctag ttccgagatt
     5101 gtaaatttct ttagagttgt cacgagatag gtgaaaaagt attttgtacg atgcgcacta
     5161 cttatcgcta ttttctaatc ttcacttttt tagtgctcgg ctgcactcaa gctaacgtcc
     5221 actttcccat tcagaaaggt gtactcgatt tacgcagcgc aaccatcgac aattcttttc
     5281 ggatcaatct cgacggcgac tgggagtttt actggcaaca attactcact cccaacacgt
     5341 ttcaaaaacc gcacgagcag caattcatcc acgtacccag ccattggagc gccgttacat
     5401 tagacaaaaa aaatctcact ggcatgggat tcggcacata ccgcttaaac attttattgc
     5461 cacccaaata tcc
//
LOCUS       DAOMPD010000146         5448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6333_length_5448_cov_2.376228, whole genome shotgun sequence.
ACCESSION   DAOMPD010000146 DAOMPD010000000
VERSION     DAOMPD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6333_length_5448_cov_2.376228"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="glyA"
                     /locus_tag="PLY93_06375"
     CDS             <1..652
                     /gene="glyA"
                     /locus_tag="PLY93_06375"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804329.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRP69139.1"
                     /translation="GLVAAGLHPSPLEYADIVTTTTHKTLRGPRGGIILTNNEEIIKK
                     INSRVFPGVQGGPLMHVIAAKAVALGEALEPSFKEYQKAVIENAAALASRLLERKFSL
                     ISGGTDNHLMIVNTFDTKGITGKDAQLMLEAAQITTNKNMLPYDKNKPAVTSGIRLGS
                     PALTTRGFTKADFSETADIIADILAEKGSDVAIGAAKERVQKLCQKYPMARFRLGT"
     gene            683..1114
                     /locus_tag="PLY93_06380"
     CDS             683..1114
                     /locus_tag="PLY93_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69140.1"
                     /translation="MRLFRYTSFFFILTSCLLAAALSTSERKAVLREMIEMDVAARNL
                     TSIIATSDKKMLDDTLQRLVSWQIQSHPEHGKPFAKALAKWKDAGALRYGNDLQKEAR
                     ALRAYANARGKFSVKDWEHTKDSFTKILTACQGCHSITKKE"
     gene            1120..2082
                     /gene="lepB"
                     /locus_tag="PLY93_06385"
     CDS             1120..2082
                     /gene="lepB"
                     /locus_tag="PLY93_06385"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRP69141.1"
                     /translation="MKKFYEIYFKLRGYVKASRFTAGLISLVWLIAFVFAIKTSVLDA
                     NNIPSPSMEPTLMIGDFLFVNKMRYTIDLPYTNIHLLRIAYPERGDIVTFTPPQDSAS
                     YVNKTLVKRVVGVPGDTIEVNNHEITVSGVKYPTTRAENAALLKTLQNGHTADVENLY
                     TEDVVDPTTKKLYVRHYIMKSKRGSETVMSSPGRKWVIPPHKYLMMGDNRDHSWDGRA
                     CEMVESKYRRACGEFNNCQADVITNYKTEQQCILIYSQNEGETWGLIDADKIHGKVYM
                     SYLSVNWGTGTNSELNPIVNLFKTITGEFTGVYVRWERTFSRIY"
     gene            2101..3627
                     /locus_tag="PLY93_06390"
     CDS             2101..3627
                     /locus_tag="PLY93_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRP69142.1"
                     /translation="MRKHSRKNKISLFSLKAKRFFLCLFVLFAAVSCGTVARADAQGE
                     FTPHRITGVKAVASGKIEVYVVTERAGDYENVAALPSFKLRENGVDKTIQQTLLPTRN
                     TPSRTVLLADLSRSLSQKQFNAFKRASLAFVDRLKPTDTVALISFHHKVRRELNFTSD
                     RELLKKRITGLKRSGKRTMLYEALLEAHKMLADAPPKKAIVLYTDGKENASRVGFADL
                     RELFGSNPIPFFIAGKGKSPALKKLIRLARISGGDAFRVHDARDLAKVFHYLTRLRTQ
                     EFILSYQSEQKGGSTIDLELAADDNTTPLLRSYTIPTGLGALLPQKKNDEKLFSANLY
                     ETIRRHMPDILLALIILLLVVLVLMLLFRRQEITVKVENQMPQAFVPAEQFNLYPEKK
                     SILEKAKLPLDYYHAWLVEKEGPHTGRKYKLTWHVVTLGFADDNSIVVDDNTISPRHA
                     KIERVENKFVLYDLLSETGTYLNNKKLLRPKELNDFDEIQLGRTKLIFRKSAVPQNET
                     "
     gene            3656..4861
                     /locus_tag="PLY93_06395"
     CDS             3656..4861
                     /locus_tag="PLY93_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRP69143.1"
                     /translation="MASKKNNYSIELRRLKMGRSEVVLLGTAHVSRESVNDVERAILA
                     EKPDKVLIELDEGRAKNIQDPNHWKKMDIVQVIKTGKIYLLFSSILLSLFQKKMGDRL
                     TSAPGAEFKKAIELSEKYNIEHEFIDREIRVTLKRAWQSVGFFGKMRLIAELVASLFV
                     SEDMENDEIEKLKEKDALQSVLDSLPASFRRIREIIIDERDRYMAQKVRDATVNAGKK
                     PKRVLVVVGAGHLRGLEEALAAEHDLTKLTAVRKPKVVRSLIAFLAPIFIIVSILTYV
                     TFAGDKKLDLAETLKAWIIIKCSVTAVITLLWAPHFLAWLGAVVVAPVSTFLPIIKSG
                     WIAALMEAIFRKPEVEDFESMAEATTKFKSFYRNRIFRIFRARLATGFSFGTCECLRL
                     YPNTTRLFT"
     gene            complement(4911..>5448)
                     /locus_tag="PLY93_06400"
     CDS             complement(4911..>5448)
                     /locus_tag="PLY93_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRP69144.1"
                     /translation="NFLAALALLEPYHLSAVQIQSALDTFLSVKRRLQIRLENKAATI
                     IEDFAHHPTAIEANLKVLRMLYPKRRLVVLIEPRSNTMVRRFFQEQLVDALSAADVIF
                     SDDIYRKEKYAADDRLDLARLENDLKARGKKFYLLPKESRTPFVIEKLQEGDVVCFMT
                     NGSFSGLIGDVVEKMGER"
BASE COUNT         1474 a         1286 c         1340 g         1348 t
ORIGIN      
        1 tggccttgtg gcggctgggc tccatccgtc gcctttggag tacgccgaca ttgtcacgac
       61 aaccacgcat aagacgctgc gcgggccgcg tggtggaata attttaacca acaacgaaga
      121 gataataaag aagattaact cgcgcgtttt tcctggtgtg caaggagggc cgctcatgca
      181 cgtcatcgcc gccaaagcgg tggcgttggg cgaggcactc gagccttcgt tcaaggaata
      241 ccaaaaagcg gtgattgaaa atgcagcggc gttggcgtcg cgtcttttgg agcgaaaatt
      301 ttctctcatt tcaggcggca ccgacaacca tctcatgatc gtcaacacgt ttgacaccaa
      361 gggcatcacc ggcaaagacg cgcaactgat gcttgaggcg gcgcagatca ccaccaacaa
      421 gaatatgtta ccgtatgata aaaataagcc ggcggttaca agcggcattc ggttgggatc
      481 ccccgcactc accacgcgcg gatttactaa agcggatttt agtgaaactg ccgacatcat
      541 cgccgacatt ttagccgaga agggatcgga cgttgccatc ggtgcagcaa aagagcgcgt
      601 gcaaaaattg tgtcaaaaat acccgatggc gcgtttccgt ttgggtacgt aaaagataaa
      661 actattatta aagaaggatt tcatgcgtct ttttcgatat accagttttt ttttcattct
      721 cacctcctgc cttttagccg ctgcgctgag caccagcgag cgcaaagcgg tgttgcgcga
      781 gatgattgag atggacgtgg ctgcgcgcaa tttgacgagc atcattgcca cctcggataa
      841 aaaaatgctc gatgacacgt tgcagcgttt ggtttcttgg caaatccaaa gccatcccga
      901 acatggaaaa ccttttgcca aagcgttggc caaatggaaa gacgcagggg cgctgcgcta
      961 cggtaatgat ttacaaaaag aagcgcgtgc gttgcgcgct tacgccaatg cgcgcggcaa
     1021 gtttagcgtg aaagattggg agcatacgaa agatagtttt actaaaatac tcaccgcctg
     1081 ccaaggctgc cacagtatca ccaaaaagga ataacaccga tgaaaaaatt ttatgaaatc
     1141 tactttaagc taagagggta cgttaaagcc agccgtttta ccgcagggct tatttcgctt
     1201 gtatggttga ttgcatttgt ctttgcgatt aaaacgagcg tgctcgacgc aaacaacatc
     1261 ccgtcgccat cgatggagcc cacgctcatg attggcgatt ttctttttgt gaataaaatg
     1321 cgctatacga ttgatttacc gtatacaaac atccacctct tgcgcattgc ataccccgag
     1381 cggggcgaca ttgtgacgtt taccccgccg caagactcgg cgtcgtatgt gaataaaaca
     1441 ctggtgaagc gggtagtggg cgttcccggc gatacgatcg aggtcaacaa ccatgagatt
     1501 accgtgtcgg gggtcaaata ccccacgacg cgtgcggaaa atgccgcact actgaaaaca
     1561 ttgcagaacg gccacaccgc cgatgtcgaa aatctttata ccgaggacgt ggtagatccc
     1621 actactaaaa agttatacgt gcgccactac atcatgaaat cgaaacgggg cagcgagacg
     1681 gtgatgtcgt cgcctgggcg taagtgggtg attccaccgc acaaatactt gatgatgggc
     1741 gacaaccgcg accacagctg ggacgggcgc gcgtgcgaga tggttgaatc gaaataccgc
     1801 cgtgcctgcg gcgagtttaa caactgccaa gccgatgtga ttaccaacta caaaaccgag
     1861 cagcagtgca ttttaattta ttcgcaaaac gaaggcgaaa cgtggggtct catcgacgcg
     1921 gacaaaatcc acggtaaagt ctatatgtcg tatttatcgg taaactgggg gaccggcacc
     1981 aacagcgagc ttaaccctat cgtgaattta ttcaaaacca tcaccggcga gttcaccggc
     2041 gtctacgttc ggtgggagag aacttttagt agaatctatt agtttccgat aataaagagt
     2101 atgcggaaac attcccgcaa gaataaaatt agcttgtttt cgctcaaagc gaagagattt
     2161 tttttatgtc tctttgtttt gtttgcagcg gtgtcgtgtg gcacggtggc gcgcgccgat
     2221 gcacaaggcg aatttacgcc gcaccgtatc accggcgtta aagcggtagc gtcgggaaaa
     2281 atcgaagttt atgttgtcac agaaagagcg ggggactatg aaaacgtggc agcgttgcct
     2341 tcttttaaac tgcgcgaaaa tggcgttgat aaaacaatcc aacaaacgtt acttcccaca
     2401 cgcaacacgc cgtcgcgcac cgtgctttta gccgatttga gccgctcgct ttcacaaaaa
     2461 caattcaacg cgttcaagcg ggcatcgttg gcgtttgtcg atcgcttaaa acccaccgac
     2521 acggtcgctc tcatttcatt ccaccataaa gtgcggcgtg aactcaattt taccagcgat
     2581 cgcgaacttc tcaaaaaacg tatcacaggc ttaaagcgta gcggtaagcg gaccatgctt
     2641 tacgaggcgc ttttagaagc gcataaaatg ctcgctgatg cgcctcccaa aaaagcgatt
     2701 gtgctttata ccgacggcaa agaaaatgcg tcgcgggtcg gctttgccga tttacgcgag
     2761 ctttttggct cgaacccaat cccgtttttt atcgcaggaa aaggaaaatc ccctgcgctc
     2821 aaaaaactca tccgcttggc gcgcatttcg ggcggcgatg cgttccgtgt tcacgacgcg
     2881 cgtgatttag cgaaagtttt ccattattta acgcgcctgc gcacgcagga atttatttta
     2941 tcgtaccaaa gcgaacaaaa aggtggatcg acaatcgatc ttgaattggc ggctgatgat
     3001 aacacgacgc cacttttgcg ttcgtacacg atccccaccg gtttgggagc tttgttgcct
     3061 caaaagaaaa atgatgaaaa gctgttttca gcgaatctct atgagacgat tcggcgacac
     3121 atgcctgata ttttgcttgc gctcattatc cttttgttgg tggtcttggt gttgatgctg
     3181 ctttttcgtc ggcaagaaat tactgttaaa gtggaaaacc aaatgccgca agcgtttgtg
     3241 cccgccgagc agtttaattt ataccccgaa aagaaaagta ttttagaaaa agcaaaactc
     3301 ccattggatt actaccacgc gtggctcgtc gaaaaagaag gtccgcacac ggggcgtaaa
     3361 tacaagctca cttggcacgt tgtcacgttg ggttttgccg acgacaattc gattgtcgtc
     3421 gacgacaaca ccatttcacc acggcacgca aaaattgaac gtgtcgaaaa taagtttgtt
     3481 ttgtacgatt tactctctga gaccggtaca taccttaaca ataaaaaatt attgcgccct
     3541 aaagaattga acgacttcga tgaaattcaa ttagggcgta ccaaactcat ttttcgtaaa
     3601 agtgcagtgc cgcaaaatga aacctaactc aacgcatcga caataaggat ttccattggc
     3661 ttctaaaaaa aataattatt ctattgaact acgccgccta aaaatggggc gtagcgaagt
     3721 ggtgctttta ggcacggcgc atgtcagccg cgagagtgtc aacgacgttg agcgcgccat
     3781 tttagcggag aaacccgaca aggtgctcat tgagctcgac gaagggcgcg caaaaaatat
     3841 ccaagacccc aaccactgga aaaaaatgga tattgtgcag gtgattaaaa ccggcaaaat
     3901 ttatctttta ttttcgtcga ttttactctc gctctttcaa aagaaaatgg gcgaccgctt
     3961 gacatccgcc cctggggcag aatttaaaaa agccatcgaa ctttcagaaa aatacaacat
     4021 cgaacacgaa tttatcgatc gtgaaatccg tgtaacgctc aaacgcgcgt ggcagagcgt
     4081 cggatttttc ggtaaaatgc ggctcatcgc cgaactcgtc gcatcgcttt ttgtttcaga
     4141 ggatatggaa aacgatgaaa ttgaaaaact caaagaaaaa gatgcgctac aatcggtttt
     4201 agattcgctc cccgcgtcgt tccgtcgcat tcgagaaatt attatcgacg agcgcgatcg
     4261 atacatggcg caaaaagtgc gtgatgcgac ggtgaacgct ggcaaaaaac ccaagcgcgt
     4321 gctggtcgtt gtgggggctg ggcacttgcg tggtttggaa gaagcgttgg cggcagaaca
     4381 cgatttaaca aaacttaccg ccgtgcgtaa acctaaagtt gtgcgctcgc tgattgcatt
     4441 cttggcgccc atttttatca tcgtgtcaat tttaacgtac gtcacgtttg cgggcgataa
     4501 aaaactagac cttgccgaaa cgctcaaagc gtggattatc atcaagtgct cggtcacggc
     4561 ggtgattacg ctgttgtggg caccgcactt tttggcgtgg ttgggcgcgg tggtggtggc
     4621 gccggtgagc acatttttgc cgatcattaa gtcgggttgg attgccgcgc tcatggaagc
     4681 aatttttcgt aaacccgagg tggaagattt tgaatcgatg gcggaggcga cgactaagtt
     4741 taagagcttt taccgcaacc gcatctttcg catctttcga gcgcgattgg ctactggatt
     4801 ttcatttggt acgtgtgagt gcttacggct ctacccaaat accacgagac tttttactta
     4861 gtatagaaga tttttagaaa ttgatacaat ttctttgaag ctaattgaga ctatctttct
     4921 cccatctttt ctacgacatc gccaatgagt cctgaaaagc tcccgttcgt cataaagcac
     4981 accacgtcgc cttcttggag tttttctatc acaaacggag tacggctctc tttgggcaaa
     5041 agataaaatt tcttaccacg cgctttcaaa tcattttcta accgcgctaa atcgagccgg
     5101 tcatctgctg cgtatttttc tttgcggtaa atgtcgtcgc taaaaataac atccgcagca
     5161 ctgagtgcat ccacaagttg ttcttgaaaa aagcggcgca ccatcgtatt gctgcgtggc
     5221 tcaatgagca caacgagacg gcgtttggga taaagcatac gtaaaacttt taagttggcc
     5281 tcaatcgcgg tggggtggtg cgcaaaatcc tcgatgatgg tcgccgcttt attctcgagg
     5341 cgtatctgga gacggcgttt gacactgagg aatgtgtcca atgcactttg aatttgcacc
     5401 gcagataaat ggtacggttc caaaagggcc aacgccgcca gaaagtta
//
LOCUS       DAOMPD010000147         5444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6341_length_5444_cov_2.790685, whole genome shotgun sequence.
ACCESSION   DAOMPD010000147 DAOMPD010000000
VERSION     DAOMPD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6341_length_5444_cov_2.790685"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLY93_06405"
     CDS             <1..385
                     /locus_tag="PLY93_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HRP69145.1"
                     /translation="YPVPAEEQEFARVLIQAALENHKAIDALIKERLVNWELSRISAV
                     SRAILRTGIAQLLYLKEEADDAVVIDEALLLTKQYDAIEAAAFINGLLDDIISYRDDK
                     KERVLPKPKPLAQKIKIKSKLKRQI"
     gene            complement(368..1537)
                     /locus_tag="PLY93_06410"
     CDS             complement(368..1537)
                     /locus_tag="PLY93_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69146.1"
                     /translation="MAEEIKGFIDIPASIVLSAEGLQLFAKSKTAVKRVPTRSGQPKE
                     GLLSQAFNAATVQKMIMSSYIDEIYYTAPEFLSKRQQIISTTNLAIYAVLYKMLSPTL
                     AKKVIESPVLREHNRRNPKAAITDFKNIPQTVMDAFLAKNKAVLARIEHEMLDGVLAR
                     ITADTQLAEEDKITRMRSLDKFVKWVDKRIWYLYLIIYQTPLKTEIEKAYIDLFYQYL
                     DTTQIATHTSNLMMEFIQNAEKAHLERLIVHNHLAPRDKVDTYLRNKDHREQVVAIAA
                     RQKIPLEITWQLHSDKMTSGKIYNIKIILSNYGLINEITRNELTQKMKTDTSGISLAD
                     FYEQPSEPGKLGAGLGLLYNSYLEDACREKGVRYFCTIFPEPKLEKTTVTLDLTF"
     gene            complement(1537..2628)
                     /locus_tag="PLY93_06415"
     CDS             complement(1537..2628)
                     /locus_tag="PLY93_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP69147.1"
                     /translation="MRKLEAKPGPIFQINEFIYRITLPQPFYAPNQVYLIRDEKTMLI
                     DSGYVESLGMLALSLRELNLAMNNIDILLYTHPHLDHITGGLLWNTYAKKTERWGHYR
                     MPQDIPDYPEFTNNWQQDTARILTRAFKDKQARNARIRRSNERWARFIKRFKTTPTRL
                     SHLAQGDSASGSQKQSSKMGDRAVRLERLLKDGDTFSTGKLHWKIYETPGHNTWHITP
                     VETTQGFAFTGDLLIGNIPAIYDNFDGNLELYQKSLLLLLSLREENPTMRFLPAHGNE
                     IENPERTIKVLQKTLGILENGILKKLVEGRKDLVILLESAFGTKIESGGLFVAALELL
                     EALLRKLIREGRVGSQVYEDGYEEFYLKH"
     gene            2723..3646
                     /locus_tag="PLY93_06420"
     CDS             2723..3646
                     /locus_tag="PLY93_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69148.1"
                     /translation="MKNIFSYALLFLFLHCRSTSTTHYNEEENHRTFSIDAAEKSYKV
                     SLFSKTYSALLSPSFSLVLVSPVLVDDSLLYKGNTPTVIPWFNARGVSVWLVHVPPQT
                     PLKKFGATVMPQITKAIRKNSTEKKWVMGGVSLGGQAMAHYLVDAKKHAQESGMDVSA
                     AFFLASPFDYAYPSSFGRRLSSKSIDIKNMRTRFLPQIPADLVTELHNLFDGANPVWS
                     ETVDPIDATKNHLRLFFTAGKIDNVAPTESVYKFYTQTIGGNTKNSPDIRLLIPGRMN
                     NYKEDYDHAMMITSEALVDEVLPEILRWINL"
     gene            complement(3636..>4142)
                     /locus_tag="PLY93_06425"
     CDS             complement(3636..>4142)
                     /locus_tag="PLY93_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69149.1"
                     /translation="HMLEEGNADLEETYNACKRVGDITDDFSLVSLSYEPVTENHNER
                     TGIRSKISQARAMVAQHDYANAIQLLENAYELSHDNHHVARALAHILYKAGRLKEAAH
                     YFDSYTENTPGDTRALKLLGTILYELGEKSRAADILTRLRIRNPSDEKINAQLDKIYA
                     ELAPALKG"
     assembly_gap    4143..4152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4153..>5444)
                     /locus_tag="PLY93_06430"
     CDS             complement(<4153..>5444)
                     /locus_tag="PLY93_06430"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRP69150.1"
                     /translation="AITVAALYSVKLESYHYLQGLVMDVIFSFIHGGFCLILSEIATG
                     SMRSECKRIMNEKKIPFTDRAVTTVRLKLAFFITLFLVTVYISSTLVYYNRDNLSAIF
                     IFTLMATATAVLMAYMIFYLIYSALKDIENSMDDLKRGGSGLIFTKSIDSEFVKVATG
                     ISDAARTIKDYQANLEQKIDERTHELQESLGNVRALKGQQDGDYFLTSLLLKPLSGNY
                     ANNRQIKVSMFTRQKKRFEFKTRTEEIGGDISLAHSIRLRDRDYTLFLNGDAMGKSMQ
                     GAGGSLVLGAVMQSIIERTKLSPKEQVLYPERWLKSVFLELQKTFESFDGSMLISVVL
                     GLADEKSGFVYFINAEHPWSVVYRSGKAFFVENDLTLRKLGTLGVAMGIRVQTYELQP
                     HDKFIIGSDGRDDLMLGNDEEGARIINEDETLILRQTE"
BASE COUNT         1352 a         1309 c         1281 g         1492 t
ORIGIN      
        1 ttaccctgtg cctgccgaag agcaagagtt tgcgcgcgtg ctcatccaag cggcgcttga
       61 aaaccacaaa gccatcgacg cgctcattaa agagcggctt gtcaactggg aacttagccg
      121 tatctctgcg gtgagccgcg ctattttacg taccggtatt gcgcagctac tttacctcaa
      181 agaagaagcc gacgacgcgg tggtcatcga cgaagcgcta ctcctcacca aacaatacga
      241 cgcaatcgaa gcggcagcgt tcatcaacgg tcttttagac gacatcatca gttaccgaga
      301 cgacaaaaaa gagcgcgtgc tccccaagcc gaaaccgtta gcgcagaaaa ttaagataaa
      361 aagcaaatta aaacgtcaaa tctaacgtca ccgtcgtttt ttctaatttc ggttcgggga
      421 aaatagtaca aaaatagcgc actccttttt cacggcaggc gtcttcgaga tacgagttat
      481 ataaaagccc caatcccgca cctaacttac cgggttcgct gggttgctca taaaaatcgg
      541 cgagcgaaat ccccgatgtg tcggttttca ttttttgtgt cagttcattg cgcgttattt
      601 cgttgattag accgtagtta gagagaataa ttttaatgtt gtaaatttta ccggaagtca
      661 ttttgtccga atgcaactgc catgtaatct ctaaaggaat tttttggcgc gccgctatcg
      721 ccaccacttg ctcacggtgg tctttattgc gcaaatacgt atcgaccttg tcgcgggggg
      781 ccaagtggtt gtgtacgatg agccgctcca gatgcgcttt ttctgcgttt tggataaact
      841 ccatcatcag gttgctggtg tgcgtggcga tttgagtggt gtcaaggtat tgataaaata
      901 aatcgatata cgccttttcg atttctgttt ttagcggcgt ttgatagata ataaggtata
      961 aataccaaat acgtttatcg acccatttta caaatttatc gagactacgc atccgcgtaa
     1021 ttttatcttc ctcggctaat tgagtgtcgg cggtgatacg cgctaaaaca ccatcgagca
     1081 tttcgtgttc aatgcgagcg agcaccgctt tattttttgc aagaaacgca tccatgactg
     1141 tttgcggtat atttttaaaa tcggtaatcg ccgcttttgg attgcgtctg ttgtgctcgc
     1201 gcaataccgg cgactcaatc acttttttgg caagcgtcgg tgaaagcatt ttataaagga
     1261 ctgcataaat cgccaaattg gtggtgctga tgatctgctg tcgtttactt aaaaactctg
     1321 gggcggtata ataaatctca tcgatgtacg agctcataat cattttctgc accgttgccg
     1381 cgttgaacgc ctgcgataac aagccctctt tgggttggcc gctgcgcgtg ggcacacgct
     1441 tgactgcggt ttttgatttg gcaaaaagtt ggagtccctc tgcggaaaga acgatgctcg
     1501 ccggtatatc gataaaacct tttatttctt cagccattag tgttttaaat aaaactcctc
     1561 ataaccgtct tcatacacct gcgaaccgac gcgcccttcg cgtatgagtt tacgcagcaa
     1621 tgcttccagc aattctaacg ccgccacaaa aagaccgcca ctctcaattt ttgtaccaaa
     1681 cgccgactca agaagtataa ctaagtcttt acgtccctcg acaagttttt ttaagattcc
     1741 gttctctaaa atacccaacg tcttttgtaa cactttgatg gtgcgctcgg gattttcaat
     1801 ttcattcccg tgcgcgggta aaaaacgcat tgtgggattt tcttcgcgta gcgaaagcag
     1861 tagcaataaa cttttttggt agagttctaa attgccgtca aaattgtcat aaattgcggg
     1921 gatgttacca atgagtaaat cccctgtgaa agcaaagcct tgcgtcgttt ccacaggggt
     1981 gatatgccag gtattgtgcc cgggcgtctc ataaattttc caatggagtt taccggtaga
     2041 gaaggtatcg ccgtctttaa gaagtctttc caagcgcacg gctctgtcgc ccattttact
     2101 actttgtttt tgagaaccgc ttgcgctatc cccttgtgca agatgcgaca accgcgttgg
     2161 cgtggttttg aaacgcttta taaagcgcgc ccaccgttcg ttggatcggc ggatgcgtgc
     2221 gttgcgcgct tgcttatctt tgaatgcgcg cgttaaaatg cgggcggtgt cctgttgcca
     2281 gttattggta aattcggggt agtcggggat atcttgcggc atgcggtaat gaccccaacg
     2341 ctcggttttt ttagcgtacg tgttccaaag caagccgccg gtgatatggt cgaggtgggg
     2401 gtgcgtataa agtaaaatgt cgatgttgtt cattgcgagg tttagttcgc gcaacgaaag
     2461 agcgagcatg ccgagcgact cgacatagcc ggagtcaatg agcatcgtct tttcatcgcg
     2521 gataagatac acttggttgg gagcatagaa cggttgcggc agcgtgatgc ggtaaatgaa
     2581 ttcgtttatt tgaaaaattg ggccaggctt tgcctccagc tttcgcatag tgtctatttt
     2641 tgagtttgag gttgtgtgga aatgagaaac caaccacaaa ggaagtagtt tacgtgagaa
     2701 ttcgtttaca taatcgacta ccatgaaaaa tattttttct tacgcacttc tttttttatt
     2761 cctgcattgc cgctccacca gcacgacgca ctacaatgaa gaagaaaacc atcgtacttt
     2821 cagcatcgac gccgctgaga aatcttacaa ggtctctctt ttttccaaaa cgtatagtgc
     2881 gctcttgtct ccgtcgttct cgttggtgct tgtctcccct gtgctcgtcg acgattcgct
     2941 cctctacaaa ggcaatacac cgacggttat cccctggttt aatgcacgcg gcgtttccgt
     3001 ctggctcgtc cacgtgccgc cccaaacgcc acttaaaaaa tttggcgcaa ccgtcatgcc
     3061 ccaaatcacc aaagccatcc gcaaaaactc taccgaaaaa aaatgggtga tgggaggcgt
     3121 ctctttggga ggtcaagcga tggcacacta tttggtcgac gcaaaaaagc acgcacaaga
     3181 gagcggcatg gatgtgtcgg cggcattttt tcttgcaagc ccattcgatt acgcatatcc
     3241 ttcttcgttt ggccgccgac ttagctcaaa gtctattgac atcaaaaata tgcggacccg
     3301 ttttttaccc cagattcccg ccgacctcgt caccgaactc cacaaccttt tcgatggtgc
     3361 aaaccctgtg tggagtgaga cggtcgatcc cattgacgcg acaaaaaacc acttgcgtct
     3421 ttttttcact gcgggcaaaa ttgacaatgt tgctccgaca gaatcggtgt ataaatttta
     3481 tactcagacc atcggcggta acacaaaaaa ctcacctgac attcgtttat taatccccgg
     3541 gcggatgaac aactacaaag aagattacga ccatgcaatg atgatcactt cagaagcgct
     3601 cgtcgacgag gtgttgcctg aaatcttacg ttggattaac ctttaagcgc gggcgcaagc
     3661 tctgcataga tcttatcgag ctgtgcatta attttttcgt cgctggggtt gcggatccgc
     3721 agccgtgtga gaatatccgc agcacgactt ttttcaccca gctcgtataa aattgtacct
     3781 aaaagtttga gagctcgagt gtcgcccggg gtgttttcgg tatagctgtc gaaataatgt
     3841 gccgcttctt ttaatcgccc cgctttatag aggatatgcg cgagcgcacg cgcgacatgg
     3901 tggttgtcgt gcgagagttc atacgcattt tctaaaagtt gaatcgcgtt ggcgtaatcg
     3961 tgctgcgcca ccatcgcgcg cgcttggcta attttggaac ggatgcctgt gcgctcgttg
     4021 tggttttctg tgacgggctc gtaagagagc gagaccaacg agaagtcgtc ggtaatatcg
     4081 ccgacgcgtt tacacgcatt ataagtttct tctaaatcgg cgttgccctc ttcgagcatg
     4141 tgnnnnnnnn nnttttcggt ctgccgcaaa ataagcgttt cgtcttcgtt gatgatccgc
     4201 gcaccttctt cgtcgttgcc gagcatcaag tcgtcgcgcc cgtccgaacc gatgataaat
     4261 ttatcgtgcg gttgcagttc atacgtttgc acgcggattc ccatcgccac acccagcgtg
     4321 ccgagcttgc gtagtgtgag atcgttctct acaaaaaacg ccttgcccga gcggtagacc
     4381 accgaccaag ggtgctcggc gttgatgaaa tacacaaagc ccgatttttc atcggcgagt
     4441 cccagcacca cagagatgag catcgagccg tcgaaactct cgaatgtttt ttggagctct
     4501 aaaaacaccg atttaagcca gcgctcggga tagagcactt gttctttggg cgaaagtttg
     4561 gtgcgttcga tgattgattg catcaccgcg cccaacacca acgaaccgcc cgcgccttgc
     4621 atcgatttcc ccatcgcgtc gccgttcaag aagagcgtgt aatcgcggtc gcgcaaacgg
     4681 atggaatgcg ccaacgaaat atcgccgcca atttcttcgg tacgggtttt gaactcaaag
     4741 cgtttttttt gccgcgtaaa catgctcact ttaatttggc ggttgtttgc ataattgccg
     4801 cttaacggtt ttaataaaag cgaagttaaa aaataatcgc cgtcttgctg gcctttgagc
     4861 gcacgcacgt tgcccaaact ttcttgcagc tcgtgcgtac gctcgtcgat tttttgttcg
     4921 aggttcgctt ggtaatcttt aatcgtgcgt gccgcgtcgg atattccggt ggcgaccttc
     4981 acaaactcgc tgtctatcga cttggtaaag ataagccccg aaccgccgcg ctttaaatcg
     5041 tccatcgagt tttcaatatc tttgagcgcg ctataaatga gataaaaaat catgtaggcc
     5101 atgagcactg cggtggctgt cgccatgaga gtaaaaataa aaattgccga aaggttatcg
     5161 cggttgtagt acactagggt cgacgagata taaacagtga cgagaaaaag agtaatgaaa
     5221 aacgcaagtt ttaaccgcac cgtcgtgacc gcccgatcgg taaacggaat ttttttctcg
     5281 ttcatgatac gtttgcattc cgaacgcatc gaacctgtag cgatttcact caaaatgaga
     5341 caaaagccac cgtgaataaa cgaaaaaata acatccatca caagaccttg caggtaatgg
     5401 tacgattcta attttacaga ataaagcgcc gcaaccgtaa tcgc
//
LOCUS       DAOMPD010000148         5403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6404_length_5403_cov_2.345737, whole genome shotgun sequence.
ACCESSION   DAOMPD010000148 DAOMPD010000000
VERSION     DAOMPD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6404_length_5403_cov_2.345737"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLY93_06435"
     CDS             complement(<1..591)
                     /locus_tag="PLY93_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HRP69151.1"
                     /translation="MNQKRFVIFLLLLIVVFSGGIFYALNSMGIIRAADIFPFLASRN
                     PVRVEDKEYPTEVEKVEFQKWEQKLIEREEALARREAELKEKQSGSDDTKRELEETRI
                     ALKAEKEKFILMQKEWEDRQKKVKDLAEKVRNMPPIKAQEMMQNWRDFDIIDVMRQMD
                     KDAETEGTASIVPYLLTLFQPDRRAERRWRGTALPGT"
     gene            complement(588..845)
                     /locus_tag="PLY93_06440"
     CDS             complement(588..845)
                     /locus_tag="PLY93_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69152.1"
                     /translation="MAMNYPTPVKKAFKKIHFSAVDIWQNLRTLKKEIQCLVRNIDNV
                     GACVVRNRRLLQGIEMKCEAVKEDDEKQCASWTHTIAGDRL"
     gene            complement(836..1279)
                     /gene="fliJ"
                     /locus_tag="PLY93_06445"
     CDS             complement(836..1279)
                     /gene="fliJ"
                     /locus_tag="PLY93_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRP69153.1"
                     /translation="MKKFKFALEGYLKVKKAHEQQKLGELAQVMRRVNAYREIEQAFE
                     LQYNSMLQTEKEKFRQVQVPISRLKDMYDYLGALKIRRDSATRHLAEMENEVSAKRNA
                     YNQARKERRVIEILREKKLAAYKLDVEKEEQKNLDEVNDKVGAWQ"
     gene            complement(1276..>1961)
                     /gene="fliI"
                     /locus_tag="PLY93_06450"
     CDS             complement(1276..>1961)
                     /gene="fliI"
                     /locus_tag="PLY93_06450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805136.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HRP69154.1"
                     /translation="SESPMNRVRAGYLATTIAEYFRDLGKNVLFMMDSVTRLAHAQRE
                     IGLSRGEPATTRGYPPSVFSLIPGLMERAGTSPKGSITGVYTVLVEGDDMNEPIADTA
                     RGVLDGHITLSRRLNSQNQYPAIDIADSISRTMGQVIDKDHLKAAQDFREIYTAYKEN
                     EETINLGAYARGADPVIDSAILLREPMKRFLRQGPDEQVGMDETRRLLREALAPLRAK
                     QTMPTRRLN"
     assembly_gap    1962..1971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1972..2672)
                     /gene="fliI"
                     /locus_tag="PLY93_06455"
     CDS             complement(<1972..2672)
                     /gene="fliI"
                     /locus_tag="PLY93_06455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HRP69155.1"
                     /translation="MIARNVSQEVNLLGKYRDIIQKTETIRSLGSVLEIQGNVVLSRG
                     PEVRLGELCHIEVNSTIDRFNRHAKAQEESYVLAEVVGFRNNTVILAPLEKIVGVSPG
                     SRVVSTGNMPAVSLTPAVLGRVIDGMGRPIDSRGMLVEGDACGLYGDPPDPLTRPLIR
                     TPLVTGVKAIDALLTIGLGQRIGIFAGSGVGKSTLLGMIAKYSTADVNVIALIGERGR
                     EVNEFIQEQLGESGR"
     gene            complement(2675..3418)
                     /gene="fliH"
                     /locus_tag="PLY93_06460"
                     /pseudo
     CDS             complement(2675..3418)
                     /gene="fliH"
                     /locus_tag="PLY93_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
     gene            3635..>3875
                     /locus_tag="PLY93_06465"
     CDS             3635..>3875
                     /locus_tag="PLY93_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRP69156.1"
                     /translation="MRYKLLLFVFVISANQLIIAQSRPNIIFILADDLGYADLSCYGR
                     KNYQTPHLDKLASEGIKFHNAYAGSALCTPSRVSLM"
     assembly_gap    3876..3891
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3892..4935
                     /locus_tag="PLY93_06470"
     CDS             <3892..4935
                     /locus_tag="PLY93_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRP69157.1"
                     /translation="PVGLWEPLTGSGRDSLVGLSPEYRPLPGLLQKNGYSTILIGKWH
                     LGFLPEFSPLVNGFDEFFGFLGGATDYISHTNPGNQPDLYQNNLPIVKEGYSTDLFKE
                     KAIQVIQREHTKPFFLCLMFNAPHWPWQAPGDSIYPLSVENWRKGGSPETFARMIKNM
                     DDAIGAVLAALDETSAANTIVIFTSDNGGENFSDMGPFAGRKALLREGGIRVPAIVRW
                     PGRITANSITDQAIITMDWSATILSLSKTNYDPLFPIDGIDLTTVCIGESKTIKRTFY
                     WRMTQRNPQKAIRDGNWKYLRDTAGEYLFDLAKDPGEKNDLKAKNRKIFAKLKRKFSQ
                     WEKTILPPVPLTP"
     gene            complement(4952..>5403)
                     /locus_tag="PLY93_06475"
     CDS             complement(4952..>5403)
                     /locus_tag="PLY93_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP69158.1"
                     /translation="SNPAPWETLIGEQHRLFKKHPGTTFIAAHFGWYPNDLAKLGKLL
                     DEMPNVVVEFGAIIAELGRQPRMAKQFFTKYQDRILFGKDSWVPEEYATYFRVLETED
                     EYFPYHKKYHAFWAMYGMGLPDDILKKIYYKNAIRIIPGIDKTQFPD"
BASE COUNT         1302 a         1344 c         1163 g         1568 t
ORIGIN      
        1 agtacctggt agtgcagtac cgcgccaccg gcgttcagcg cgacgatcgg gttgaaataa
       61 ggttaaaaga tatggaacga tggaagcggt gccctcggtt tcggcatctt tatccatttg
      121 ccgcatcaca tcgatgatgt caaagtcgcg ccaattctgc atcatctctt gtgcttttat
      181 gggaggcata ttgcgcactt tttcggctaa gtctttgact ttcttttgtc tatcctccca
      241 ttctttttgc atgagtataa acttctcttt ctcggcttta agagcaatac gcgtttcttc
      301 taactcgcgt tttgtatcat cactgcccga ttgcttttct ttgagctctg cttcacggcg
      361 cgcgagtgct tcttctcttt caatgagttt ttgttcccat ttttggaatt ctactttttc
      421 aacttcagtg gggtattctt tgtcttcgac gcgcacgggg tttctggagg caagaaaagg
      481 gaaaatgtct gccgcacgaa tgatacccat cgaattgaga gcgtaaaaaa tacctcctga
      541 aaaaacgacg atcagcaaaa gtaaaaaaat aacaaagcgc ttctggttca taagcgatcc
      601 cctgcgattg tgtgcgtcca tgaagcgcat tgtttttcat cgtcttcttt tactgcttca
      661 cacttcattt caataccttg taagagacgc cgattgcgta caacgcatgc gccgacatta
      721 tcaatattgc gtacaaggca ttgaatttct tttttaagag tgcgtaggtt ttgccaaata
      781 tccaccgctg aaaaatgaat ttttttgaac gcctttttta ccggtgtggg ataattcatt
      841 gccatgctcc tactttgtcg tttacttcgt ccaaattttt ctgctcttct ttttccacat
      901 ctaacttgta cgctgcaagc ttcttttctc gcaaaatttc aatcacgcgc ctttctttcc
      961 gcgcttgatt atacgcattg cgtttagcgc tcacttcatt ttccatttct gctaagtgtc
     1021 gagtcgcact gtcgcgtcgt atttttaatg cgcctaaata atcgtacata tctttaagtc
     1081 gggaaatcgg cacttgtacc tgtctaaact tttctttttc cgtttgcaac atcgagttat
     1141 actgaagctc gaatgcctgt tctatttcgc ggtatgcatt cacacggcgc atcacctgcg
     1201 caagttcacc cagtttttgt tgctcgtgcg ctttcttgac tttcaaataa ccttcaaggg
     1261 caaacttaaa tttcttcaat tcagcctcct tgttggcatc gtctgtttag cgcgcagcgg
     1321 tgcgagggct tctcttaaaa gccggcgcgt ctcatccata cccacctgct cgtcggggcc
     1381 ttgacgcaaa aaacgcttca tgggttcgcg caataaaatt gcactatcga taacagggtc
     1441 ggcgccgcgc gcatacgcgc ccaagttaat cgtctcttcg ttttctttat acgcggtata
     1501 gatttcacga aaatcttgcg ccgctttcaa atggtctttg tcgatgactt gccccatcgt
     1561 gcggctaatc gaatcggcaa tgtcgattgc gggatattgg ttttggctat tgaggcggcg
     1621 cgataaagta atgtgaccat ccaacacacc gcgtgccgta tcagcgatgg gttcgttcat
     1681 gtcgtcgcct tcaacgagaa ccgtataaac tccggtgata cttcctttgg ggctggtgcc
     1741 cgcacgctcc atgagacccg gtatcaatga aaacaccgac ggtggatatc ctcttgtagt
     1801 tgcaggctct ccgcgcgaca aaccaatttc acgctgcgca tgcgccaagc gcgtgacaga
     1861 gtccatcata aataaaacat ttttgcccaa gtctctaaag tattcggcga tcgtcgtcgc
     1921 caaataaccc gcgcgcacac gattcatggg tgactcggat gnnnnnnnnn nttgcgccca
     1981 ctttcgccaa gttgctcttg gataaattca ttcacctcgc gtccacgctc accaatgagt
     2041 gcgataacgt tcacatcggc ggtggagtat ttggcaatca tacccaggag cgtcgattta
     2101 cccacgccag agcccgcaaa aattccaatc cgttgtccca atccgatagt gagtaatgca
     2161 tcgatggctt taactcctgt taccaacggc gtgcggatta aaggacgcgt caatgggtcg
     2221 ggaggatctc catagaggcc acatgcatcg ccttcgacga gcatgccacg actatcgatg
     2281 gggcgcccca tgccgtcgat cacgcgcccc agaacagcgg gtgtgagcga aaccgctggc
     2341 atatttcctg ttgaaaccac gcggcttccc ggtgaaacac ccacaatttt ttccagcggc
     2401 gctaaaataa ccgtattgtt gcgaaagccc accacctcgg cgaggacgta actttcctct
     2461 tgcgctttgg catggcgatt gaaacggtcg atggttgagt tgacttcaat gtggcaaagt
     2521 tcacccaagc gcacttctgg accgcgcgat agcacgacgt taccctggat ttcaagcacc
     2581 gaccccaacg agcggatcgt ctcggtcttc tggattatgt ctctatattt accgagaaga
     2641 ttgacctctt gactgacgtt gcgcgctatc ataattataa cggtttggcg tcgcggattg
     2701 cctgctcaat ctctttaagc tgcgttgaaa tacgcgcatc gattgcgccc acgtcggttt
     2761 cgatgatgac accaccacgg tcgacgcgcg agtcttcgta gatgttcact ttgcgtagcg
     2821 actccatcat tttcactatt tcgtctttgt gcgctgtggt gagttccaag tcggcaaagt
     2881 tgacacggat atcgatgcgg tcacgttctt taatgcgcgc caagccttcg cgaatattgt
     2941 tgagcaccac ttctttacgt tcgataattt catctttgat cactttgcgc gcaatcatca
     3001 aaatcatttc gaccatgagt ttttccgatt ctttgatgat ttgttcgcgt acatcaatcg
     3061 cattgcctaa aatggtaccc aagcgatcga tgaggcggcg tacctcgccc atcccttctt
     3121 tatatccttg ttcgcggcca gcgttgtatc ctttttggta cgcctcgtgg ttgatatctt
     3181 gaacacgcag ctcggcttct ttcaccatgc gttcaacttc gagcttggca cgctcgatga
     3241 tttggttcga ttgcaatcgc gccgactctt cttctttgcg cgctctttct tttgcgtctt
     3301 gaattaaacg aaacgcttct gctttaccgt cttcgctgat ccgttgcgcg tctttttcag
     3361 cttctttttg gcggattttt atttcttgtt cggttttttc aggaaatgcc tgccggagat
     3421 tgtcaaagac cacttttttg cggtatccta tgaccgagta catcaaaaaa gccaccaggt
     3481 cagaaaacac gtataaaacc ggcataggca gcagggagaa aacatagagc attccgaaaa
     3541 caacttgata catccttttg aagattccta tgcaagttaa tttttaatcc aattcccctt
     3601 aaaacatcct aactttctta cattaagttc ttgtatgcgc tacaaacttc tgctatttgt
     3661 ttttgtgatt tcagcaaacc aactgataat tgcccagtcg aggccaaata tcattttcat
     3721 cctggctgat gatcttggat acgcagacct tagttgttat ggccgcaaaa attaccagac
     3781 tccccatctg gataaactgg cttcagaggg aataaaattt cacaatgcat atgccgggtc
     3841 ggcactttgt acgcccagtc gcgtgtcatt aatgannnnn nnnnnnnnnn ncccgttggt
     3901 ctgtgggagc cattgacggg ttcaggtcgc gatagtcttg ttgggctttc ccccgaatac
     3961 agacccctgc caggcctgtt acaaaaaaac ggttattcga caatccttat tggaaaatgg
     4021 catttaggtt ttctgcctga attcagcccc ctggtaaatg gctttgacga attctttggt
     4081 tttcttggcg gggctactga ctatatatcc cataccaatc ctgggaacca gccagatctt
     4141 tatcaaaata acctgcccat cgtgaaagaa ggatacagta cggatctttt taaagaaaaa
     4201 gccatacagg taatacaacg ggaacataca aagccttttt ttctctgcct catgttcaac
     4261 gccccacatt ggccatggca ggctcctggc gatagtatct atccactgtc tgttgaaaat
     4321 tggcgaaagg gtgggtcacc ggaaacattt gcccgcatga ttaaaaatat ggacgatgca
     4381 attggggctg tccttgcagc actggacgaa acgtcagctg ccaatactat tgtgatcttc
     4441 accagcgata atggcggtga aaatttttcg gacatggggc cgtttgctgg aagaaaagcc
     4501 ctcttacgcg aaggaggtat ccgggttcca gccattgtac gctggcctgg aaggataacc
     4561 gccaacagta ttacagacca ggcaatcatt accatggatt ggagcgccac cattctatct
     4621 ctttcaaaaa caaattatga tccactattc ccaattgatg gcattgacct gacgacggtt
     4681 tgtatcggtg aatcgaaaac tataaaaaga acattctatt ggcgaatgac gcaacgtaac
     4741 ccccaaaaag caattcgaga cggaaactgg aaatacttac gcgataccgc cggtgaatat
     4801 ctttttgatc tcgccaaaga tccaggtgaa aagaatgatt tgaaagcaaa aaacagaaaa
     4861 atctttgcaa aactaaaaag gaaattcagc caatgggaaa agactatttt accacctgta
     4921 cccttgactc cataaaaccc atccctaaaa atcaatccgg gaactgtgtc ttgtcgatac
     4981 ccgggatgat ccgtattgca ttcttatagt agattttttt caggatatca tcaggtaatc
     5041 ccattccata cattgcccag aatgcgtggt attttttgtg gtatggaaaa tattcatctt
     5101 cggtttccaa aaccctgaaa taggtcgcat attcttcagg cacccaactg tcttttccaa
     5161 acagtatcct gtcctggtat tttgtaaaga actgctttgc catgcgtggt tggcgcccaa
     5221 gctcagcgat gatcgctcca aattctacca ctacattggg catttcgtcg aggagcttgc
     5281 ccagtttggc gagatcattg ggataccatc caaaatgtgc ggcaataaaa gtcgtcccgg
     5341 gatgcttttt aaaaaggcgg tgctgctctc ctattagcgt ttcccacggc gccgggtttg
     5401 aat
//
LOCUS       DAOMPD010000149         5381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6435_length_5381_cov_2.913068, whole genome shotgun sequence.
ACCESSION   DAOMPD010000149 DAOMPD010000000
VERSION     DAOMPD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_5381_cov_2.913068"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLY93_06480"
     CDS             <1..777
                     /locus_tag="PLY93_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69159.1"
                     /translation="FLNIVFFEFADALRSRWMIFYALVVFSFSLLLLSMASGSADVAA
                     SLLNFILLIVPLFILIYGVVAFHNSLPFQRVIIALGVKRTTIYTGQFLGRLAGLLPGF
                     IGGLLLAFIFGGAIEGFTSLMLILVYGVLLNAVFLALAFFLSQLSERIELLVAFAIGI
                     WFILYILYDSIILVLVIFMGDFPLEGALLTLTFLNPIDTARAVVLMQSNLQSLMTHSS
                     AVYAKVLSGYIGVSIGTLVLCIQACVVTLWGLCLYKHRNL"
     gene            complement(923..1171)
                     /locus_tag="PLY93_06485"
     CDS             complement(923..1171)
                     /locus_tag="PLY93_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69160.1"
                     /translation="MKTKLILILSVFLLATACGKKSAATGDEGCSDLDAMSARLRCTN
                     NNLEFCSSYSKYKWMQTQKCDTSKTCFVAPDGKSGGCK"
     gene            complement(1319..1729)
                     /locus_tag="PLY93_06490"
     CDS             complement(1319..1729)
                     /locus_tag="PLY93_06490"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRP69161.1"
                     /translation="MVKLPKNAVAALHKQALVAAAHAYAPYSNIHVGAALLANDGNIY
                     TGCNVENASYGLTVCAERNAIANALIQITQNRATLIQAMYVVSDKLGAMPPCGACHQV
                     ISEFGKGGWIFFRTEKSIKKLKIAALLPEAFNLN"
     gene            complement(1732..2733)
                     /locus_tag="PLY93_06495"
     CDS             complement(1732..2733)
                     /locus_tag="PLY93_06495"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRP69162.1"
                     /translation="MIAKSVAIAGASGAVGIEFMSVLEKLDFPVKSLKLLATKRSAGK
                     KLKFKGEDIVVEEMTPQSFKGVDVALFSAGSSVSKEYEKYTLDAGCVMIDNSSAFRMR
                     EGTPLVIPEINPEDAAKHKGTIANPNCSTIIMLMGVYPLLKKYPAKRLVVSTYQAASG
                     AGWAAMEELKNSTRAVLDEKPFTPEKMPYPYGFNLFSHNATVGENGYNEEEMKMVNET
                     KKILHNEKMGVSATCIRVPVLRAHAESINIEFEGKAPSEEEARALFAAFPGVKVVDDR
                     AKNHFPMPLEASGQFDCLVGRVRKDLSMPDKALNVFIVGDQLLKGAALNTVQIATLL"
     gene            2788..3489
                     /locus_tag="PLY93_06500"
     CDS             2788..3489
                     /locus_tag="PLY93_06500"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRP69163.1"
                     /translation="MNKDFSESWCGYYPERRARIHSIDDSKHELNQTQVTRLLQKGYR
                     RYAGHYYKNYCRTCHECIPYRVLVDEFKPNRAMRRTFQRNADLTLQWYTPLPTEEKFQ
                     LYLKYQLARHKSNEEYSRSLRKILLQAMIEQMYTNPIDSIELTIYKEEKVIGFAIFDV
                     LEDSLSAVYSVFDPDYHVRSLGTFNILQSIEKTCALGLPYLNLGLYLEFHEKMNYKSK
                     FTPAEIYINHAWRRL"
     gene            3573..4844
                     /locus_tag="PLY93_06505"
     CDS             3573..4844
                     /locus_tag="PLY93_06505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRP69164.1"
                     /translation="MPIPAHYANLIALAVPIFFISIAIEFFVSLAMKRRIYRINDSIA
                     DLSAGMLQELVGVFTKSIRFGLYILIFEAVHKPTAATNAAYGLPINAATWIALFILVD
                     VAYYWFHRHAHTFAVMWGSHEAHHSSQEYNFAVALRQGAFQGLFSFPYYLPLAIVGFT
                     PIQFLVCTQLNTLYQFFIHTRLVGKLGFLEYFMNTPSHHRVHHGVNPKYIDKNHAGVF
                     IVWDKLFGTFQVEEEEPCYGTVKPLNTFNPIWTQLHYFWHLIKMTYHARGMDKLKVWI
                     KGPGWKPDYLGKIEPVPEVDAATYQKYDAPNALTLKVYATVVFLLSLMPFLILQRNTQ
                     LPLANQLLLASLLLLGLATAGGLMMRQRWSFVLEIIRIFVFAAAALGIVSVLTQKNFY
                     TASILIISVSAVQLLLLFFTLRYSGRKTQIA"
     gene            4837..5307
                     /locus_tag="PLY93_06510"
     CDS             4837..5307
                     /locus_tag="PLY93_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRP69165.1"
                     /translation="MRKRHSPKVRLAVKERAIQFVSRQMRSVHELRQKLLDEEYAVDE
                     IETALERLKEIHLLNDVALAETIARQYRDRGNRFIAQKIKQKGIVLDNPSSAFEALDD
                     EEERAYAAATKKMRSLAGLEKHKIAEKLYAHLALRGFSSSIAQRTARKIVDVME"
BASE COUNT         1312 a         1292 c         1234 g         1543 t
ORIGIN      
        1 tttttaaaca tagttttctt tgaatttgcc gatgcgctca ggagtcgttg gatgatcttt
       61 tatgcgctcg tcgtttttag tttttctttg ttacttttgt cgatggcttc gggttcggca
      121 gacgtggccg catcgctttt aaattttatt ctacttattg taccgctttt tattcttatc
      181 tacggggttg tggcattcca taactctctc ccttttcaac gcgtgattat cgctctgggc
      241 gtcaaacgca ctacaattta taccggacaa tttttaggta ggctcgccgg acttctcccc
      301 ggttttatcg gcggtctttt gctcgcattt atttttgggg gcgctatcga aggttttact
      361 tcgttgatgc ttattttggt ttatggtgtt ttgctcaacg ccgtttttct cgcgctggct
      421 ttttttcttt cacaactaag cgaaagaatc gaactactcg tcgcttttgc aataggtatc
      481 tggtttattc tttatatttt gtacgattcg attattctgg ttttagttat ttttatggga
      541 gactttcccc tcgaaggcgc cctattaaca ctgactttct taaaccccat cgacacagcg
      601 cgggctgttg tattgatgca aagcaatctg caatcgttga tgacgcattc gtcggcggtg
      661 tacgcaaaag tgctttcagg ttatatcggt gtatccattg gcacgctcgt gctttgcatt
      721 caggcatgcg ttgtcacgtt gtggggattg tgcttatata agcatcgtaa tctataaaga
      781 attttatata ggcagcattt taccgatttc atgattttca aaagcgcacc gcgtacaaaa
      841 atttgcaaaa aattgccttt ttgtacacgc tgcgcgagtt ggcacacagg ctcttaagcg
      901 ttcgcgagga aaagttttgg cgttatttac atccgcctga tttaccatcg ggtgcgacaa
      961 aacaagtttt gctcgtatcg cacttttgtg tttgcatcca tttatatttt gagtacgaag
     1021 agcagaactc aagattgtta ttggtacaac gcaggcgtgc actcattgca tctaagtcag
     1081 aacacccctc gtcgccggta gcggcagatt ttttgccgca cgctgttgca agtaaaaata
     1141 cgctcaatat taaaattaat tttgttttca tagtcacgct attttcttca aagatatgct
     1201 ttggcaagag aaattcttgt ttgggttgca gtggttgaga gcttgtgcgc caactcgcac
     1261 agcgtgtgca aaaaaattgt ctcagcgagt tggcgcatag gcttttgaca acaccccctc
     1321 aattcaaatt aaacgcctcg ggtaacaacg cggcgatttt taatttcttt atactttttt
     1381 ctgtacggaa aaaaatccat ccgcctttgc caaactcgct aatcacttgg tggcaagcac
     1441 cgcacggtgg catcgcgccg agtttgtcgc tcaccacgta cattgcttgg atgagcgtcg
     1501 cacgattttg tgtaatttgg atgagcgcat tagcgatggc gttgcgttca gcgcaaacgg
     1561 tgaggccata cgaggcgttc tccacgttgc agccggtata gatgttgccg tcgtttgcta
     1621 aaagcgctgc gccgacgtgg atgttcgagt atggcgcata cgcgtgtgcc gcagcgacca
     1681 aggcttgctt gtgcagcgcg gcgacggcat tcttggggag tttaaccata tttaaagcag
     1741 tgtcgcaatt tgtacggtgt tgagcgctgc acctttcaaa agttgatcgc cgacgataaa
     1801 gacgttgagc gctttgtcgg gcatactcaa atctttacgc acgcgcccca cgaggcaatc
     1861 aaattggcct gacgcttcca acggcatcgg gaaatgattc ttagcgcggt cgtcgacgac
     1921 tttcaccccg gggaaagcgg caaaaagtgc gcgtgcctct tcttcggaag gcgccttacc
     1981 ttcaaactcg atgttaatcg actcggcgtg cgcgcgcaac acaggcacac ggatacacgt
     2041 tgcgctcacg cccatttttt cgttgtggag aattttttta gtttcgttca ccatcttcat
     2101 ttcctcttcg ttgtaaccgt tttcacctac agtagcgttg tgcgaaaaaa gattgaaccc
     2161 ataagggtat ggcatttttt cgggggtaaa gggtttttca tcgagaacag cgcgtgtgga
     2221 gtttttgagc tcttccatcg cggcccaacc cgcgcccgag gcggcttggt atgtggaaac
     2281 cacaagacgc ttcgcagggt atttttttaa aagaggatac acacccataa gcatgataat
     2341 cgtcgagcag ttgggattgg cgatggtgcc tttatgcttt gcagcgtctt ctgggtttat
     2401 ttcaggaatt accagcggcg tgccttcgcg catgcgaaac gccgagctat tgtcgatcat
     2461 cacgcagccc gcgtcaagag tgtacttctc gtattctttg ctcaccgagc tgcccgcaga
     2521 aaaaagagcg acatcgacgc ccttaaacga ctgcggcgtc atttcttcga cgacaatatc
     2581 ctcgcccttg aatttgagtt ttttacctgc acttcttttc gtcgctaaaa gtttgagcga
     2641 tttcaccgga aaatctaatt tttctaaaac gctcatgaat tcaataccaa ccgcgcccga
     2701 ggcgccggcg atggctacgc tttttgctat catgcgctat ccattttttg cgagacaggg
     2761 tgaaaatcga aacacaaacg gattttagtg aacaaggatt tcagcgagtc gtggtgtggg
     2821 tattatcctg aacgtcgggc gcgcattcac agcatcgacg attcaaaaca cgagctaaat
     2881 caaacgcaag taacgcggct cttacaaaaa ggctaccggc gctatgcagg ccattattat
     2941 aagaattact gccgcacctg ccacgagtgc atcccatacc gcgttcttgt cgacgagttc
     3001 aaaccgaatc gtgccatgcg acgcactttc caacgcaacg ccgatttaac attacagtgg
     3061 tatacccctt tgcccacaga agagaaattt caactttatc tgaaatacca attagcgcgg
     3121 cacaaatcca acgaagagta ttcacgctcg ctgaggaaaa ttcttttaca agcgatgatt
     3181 gagcaaatgt ataccaaccc catcgattcg attgaattga caatatataa ggaagaaaaa
     3241 gtaattggct ttgccatctt tgacgtgctc gaagactcgc tctcggcagt gtattcagtg
     3301 ttcgaccccg attaccatgt gcgttcgttg ggcacgttta acatactcca aagcattgaa
     3361 aagacatgcg cgttgggact tccctatctc aatttagggt tatatcttga gttccacgaa
     3421 aaaatgaatt acaaatccaa gtttactccc gctgaaattt atatcaacca cgcgtggcgg
     3481 cggttatagc ttggttaact atctaaagag ctaccgataa cgactctatt ttttacatga
     3541 cgcagaatgc gcacttcgca gtaaatccca ctatgcccat acccgctcac tacgctaatc
     3601 tcattgcgct tgcagtgccg atttttttta tttcaattgc gattgaattt tttgtatcgc
     3661 tcgcaatgaa acgacgtatt taccggatca acgattccat cgccgacctg agcgcgggca
     3721 tgctgcaaga gctggtcggt gtttttacaa agtctatccg ctttgggctt tacattctta
     3781 tttttgaagc ggtccataaa cctaccgccg ccaccaatgc tgcgtatgga ctccccatca
     3841 atgccgcgac atggattgcg ctttttattc ttgtcgatgt ggcgtactat tggttccacc
     3901 gccacgcgca cacgtttgcc gtgatgtggg gttcccacga agcgcaccac tcgagccaag
     3961 aatataattt cgcggtggcg ttgcggcaag gcgcgttcca aggattattc tcatttcctt
     4021 actatttacc gcttgccata gtgggattta ccccgataca gtttttagtc tgtacacaat
     4081 taaatactct ctatcagttt tttatccaca ctcgtctggt cggtaagttg ggttttctcg
     4141 aatactttat gaacacgcca tcgcaccacc gcgtccatca cggtgtcaat cccaagtaca
     4201 tcgacaaaaa ccacgccggt gtttttattg tgtgggataa gctctttggc acgtttcaag
     4261 tcgaggaaga agaaccgtgt tacggcaccg tcaaaccact caacacgttt aatcccattt
     4321 ggacgcagct tcactacttt tggcatctca tcaaaatgac gtaccacgcg cgcgggatgg
     4381 ataaactcaa agtgtggatt aaagggccgg ggtggaaacc cgactattta ggaaaaatag
     4441 aacctgtgcc agaggtcgac gccgccactt atcaaaaata cgatgcgccg aacgcattaa
     4501 cgcttaaagt ctacgcaaca gttgtattcc tgttatcgct tatgccattt ttaatcttac
     4561 agcgtaacac gcagttgccg cttgcaaacc aactcttgct ggcgtcgttg ctccttctcg
     4621 gtttggcaac cgctggcggc ctcatgatgc gtcaaaggtg gagttttgta ctcgaaatca
     4681 ttcgtatttt tgtgtttgcc gccgccgcgt tgggaatagt ctcagtgctc acgcagaaaa
     4741 acttttacac cgcttctatt cttatcatct ctgtgtcggc ggtacagtta ttgctgctat
     4801 tttttacgtt gcgttatagt ggacgaaaaa cacaaattgc gtaaaagaca ttcccccaaa
     4861 gtacgccttg cagtgaaaga gcgcgcgatc cagtttgtgt cgcgtcaaat gcgcagcgtg
     4921 cacgaacttc ggcaaaaatt actcgacgaa gaatacgctg tcgacgaaat cgagacggcg
     4981 ctcgaacggc tcaaagaaat tcatttattg aacgacgttg ctctggcaga aacgattgcc
     5041 cggcaatacc gcgaccgtgg caatcgcttt atcgcgcaga aaattaaaca aaaaggcatc
     5101 gtgttagaca acccctcgtc ggcgtttgaa gcgttggacg acgaagaaga acgcgcgtac
     5161 gctgccgcca caaaaaaaat gcgctcgttg gcgggattag agaaacataa aattgcagaa
     5221 aagctgtatg cgcacctcgc cctgcgcgga ttctcgagca gtatcgcgca gcgtaccgca
     5281 cgaaaaattg tggatgttat ggaataagtt tttggtaagc gtcgagtaaa aacgatttat
     5341 cccctgaaag cgtgacatgg cctaatttac ggaaagggcg c
//
LOCUS       DAOMPD010000150         5359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6462_length_5359_cov_2.781109, whole genome shotgun sequence.
ACCESSION   DAOMPD010000150 DAOMPD010000000
VERSION     DAOMPD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_5359_cov_2.781109"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLY93_06515"
     CDS             complement(<1..790)
                     /locus_tag="PLY93_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP69166.1"
                     /translation="MASSHSIKVLKGPRQAGKTSLLVRLGGYTFVSLDDIHQRALAQE
                     DPVFFLDQFTGKILLDEATLVPALFPELKRRADAFKAARLAGKKDHAPFDVWITGSNQ
                     TLLQKSVRESLAGRASYFDLNTLSLHEMPKAPLADFFMKGGWPELHANVALDAKRYLD
                     DLISTFIEKDIVVAAGIERKAAFLKATALAAGRVGQLFNASDIARNTGVETTTVQSWM
                     QILQDNGIVRLLQPYFNNRSQRFIKTPKIYFQDTGLATRLQGWSE"
     gene            complement(928..1551)
                     /locus_tag="PLY93_06520"
     CDS             complement(928..1551)
                     /locus_tag="PLY93_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP69167.1"
                     /translation="MKKLKIASLFAVFALIATPALMADGLEVKAGVGSVKAASDKVGF
                     DAGVGYSIGLERFFAIVPEVNFNWLNFDGALGGGSAGQTGLSGASTPSSSFYTLPVLL
                     NARILIPMGGDETPAVQPIITAGAGYGWSHYSYSYSGTSIKADLSGFMYQASLGLLIN
                     LGMISEGSASSTNILVEAGYRGGTLATSGTNYDWSGYVIRAGVNLAL"
     gene            complement(1668..2513)
                     /locus_tag="PLY93_06525"
     CDS             complement(1668..2513)
                     /locus_tag="PLY93_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69168.1"
                     /translation="MKRALLLFLVLTFGVAAKTPFNTKTVGRKNYAAILAAKAFAWLA
                     ANQDEKTFGIDVVLLYTFTMHKFNSPDLSITHLVREKARRGYYSSKEGSPFLHVISPL
                     EGTPLQVSERINIDAMTSTALWCDRKQPFDGYLDKLRSMGRMGKYELTHMYWAILMMK
                     EKECAFFQGNQSALEALEKEFVRDLHALVARGAVDDLTIEATTFLYYGSAATKATEDY
                     LIGILPAMERAFDNAKTTRLSAHTVALFYWGLLEYLNPDLPRVPLAVRFRVGMGTVRH
                     DNRKI"
     gene            complement(2518..3771)
                     /locus_tag="PLY93_06530"
     CDS             complement(2518..3771)
                     /locus_tag="PLY93_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69169.1"
                     /translation="MKKISKKWRYLAWAFSLTFVACANESIPQGQSMGAAVTVEKGRF
                     IDAPVTGLSYKTATQSGVTDSDGYFKYIAGENVEFSLGKTILGSAKGAGIVTPEDFAA
                     DGLANQKVKNTLRLLQTLDSDGNNANGIVIKEEVRNLLAASVIDLSKATAAFEADTLV
                     QAVVLLARGTATPLVSEVDALSHYLSSRLSGSFKDTYNGQHVLTATAWQLKDSWTDQT
                     DSVVKIDGVLRYIILQKSASDAYNANKYQKVVYIPNNDGSLYTCTLSPFDSATQAAAE
                     AITDTTDKTDPATKGCSGFSWSKLTPVVNPVMGNWKDDWGNSFSIGAILWSSPWGDAD
                     TIVLYNAIDSYFIIQKPSNDAYNPSKYQKVILTKKASDYFTCTLSPFNSTTQVTAMAI
                     ADTSDKTDPATGGCDGFSWSKLIPN"
     regulatory      complement(3847..4037)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4054..4842)
                     /locus_tag="PLY93_06535"
     CDS             complement(4054..4842)
                     /locus_tag="PLY93_06535"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRP69170.1"
                     /translation="MTTLQAVILGIVEGLTEFLPMSSTGHMILADSMMQIQDPEFVKT
                     FEIAIQLGAIAAVALLYIRRFLKEPRLYFILFIAFLPTGVCGLLFYKYIKTLLFNPLS
                     VSIALILGGVVLLFFDKLAQNRDEKHKTHEIQITPRTAFIIGLFQTISMIPGVSRAAA
                     TIGGGLVAGLSRVRAVEFSFLLAVPTMVVATAYDLYKNRETLNAAHLDVLVIGALVAF
                     ATALLAVRFFVAFISKRGFTAFGIYRIVVGIIFLVVFWGKTLTK"
     gene            complement(4928..>5359)
                     /locus_tag="PLY93_06540"
     CDS             complement(4928..>5359)
                     /locus_tag="PLY93_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP69171.1"
                     /translation="NIEAIFELRRFEKSLIKEETDKLLKELEIEHVQNQNGITLSGGE
                     RRRAEIARALASDPKFLLLDEPFAGVDPIAVKEIQRVVWQLQERGLGILITDHNVRET
                     LKLTDRAYIIFQGQVLIEGTADILINDKKAREIYLGDDFKI"
BASE COUNT         1434 a         1299 c         1245 g         1381 t
ORIGIN      
        1 actcactcca cccctgtaag cgcgtggcaa gccctgtgtc ttgaaaataa attttgggcg
       61 ttttaataaa gcgttggctg cggttattaa agtaaggctg caatagccgc acaatgccgt
      121 tatcttgcaa aatttgcatc cacgactgta ccgtcgtggt ttcgacgccg gtattgcgcg
      181 caatatccga agcattgaaa agctgcccca cccgcccggc ggcaagcgcg gtcgctttca
      241 aaaacgccgc tttgcgctcg atacctgcgg caacgacaat atctttttca ataaaagtag
      301 aaatcaagtc gtcgaggtaa cgttttgcat cgagtgctac attcgcgtgt aattctggcc
      361 atccgccttt cataaaaaaa tcggcgagcg gtgctttggg catttcatgc aatgaaagcg
      421 tattcaaatc gaaatagctc gcgcgccccg ccaacgattc gcgcaccgat ttttgcaata
      481 aggtttggtt tgagccggta atccacacat cgaatggggc atggtctttt ttacccgcta
      541 agcgcgccgc cttaaacgcg tcggcacggc gttttaattc gggaaaaagc gcgggcacca
      601 gcgtcgcttc gtcgagcaag attttacccg taaattgatc gagaaagaaa acggggtctt
      661 cttgtgccag agcgcgttgg tgtatatcgt cgagcgaaac gaatgtataa ccgcccaaac
      721 gcaccaagag agaagtcttg cctgcttgcc tcggcccttt gagcacttta atggagtgcg
      781 agcttgccac agcagtacgc aaaaatttta gtatgtcgcg gtatatccac acctgactaa
      841 tttagtctca gaaactaaat tagtcaacat tttttgcata aaaaagcggg cttacgcccg
      901 ctttttttat gcagtatttg cgttggatta gagagccaag ttcacaccag cgcggataac
      961 ataccccgac cagtcgtaat ttgttccgct tgtcgcaaga gtaccaccac ggtaaccggc
     1021 ttcaacaagg atatttgttg aagaagctga accttctgaa atcatgccta aattgataag
     1081 taaaccaagc gacgcttgat acatgaaacc agaaagatcc gctttgatag aagttcctga
     1141 gtagctatac gaataatgcg accaaccata gccagcacct gctgtaatga taggttgaac
     1201 cgctggtgtt tcatcgccgc ccatggggat taaaatacgt gcattcaaaa gaaccggcaa
     1261 ggtgtagaaa cttgacgatg gagttgaagc acctgaaaga cctgtttgac ctgccgaacc
     1321 accgccaagg gcaccatcaa aatttaacca gttgaaattc acctctggca caatcgcaaa
     1381 aaaacgctct aaaccgatag aataaccaac accagcatca aagcctactt tgtctgatgc
     1441 cgctttcacc gaacccacgc ccgctttgac ttctaaaccg tcggccatga gtgcaggtgt
     1501 tgcaatcaat gcaaataccg caaaaagcga tgctattttt aattttttca tattttattc
     1561 tccttaaaat ctcaatgttg agctgttact cactccacat tattagacga ttaaccccgt
     1621 cttttaggtc attaaaaatt gcctaaaaaa tgctgtgggt ttgtaaacta tattttgcgg
     1681 ttatcgtgtc ggactgtccc cataccaact cgaaaacgaa cggccaacgg cacgcgtggg
     1741 agatcggggt ttaaatactc aagcaacccc cagtaaaaaa gtgcgacggt gtgcgcactc
     1801 agacgtgtcg ttttggcgtt atcgaatgcg cgttccatcg ccggtaaaat accaatgaga
     1861 taatcctctg tcgcctttgt tgccgcactg ccataataaa ggaacgtggt agcttcgatg
     1921 gttaaatcgt cgacggcgcc gcgtgcaacg agcgcgtgta agtcacgcac aaattctttt
     1981 tctaatgcct cgagcgcgct ttggtttcct tgaaagaacg cgcattcttt ttccttcatc
     2041 attaaaattg cccaatacat gtgcgtgagt tcatatttgc ccatgcgtcc catagagcgc
     2101 agcttatcga gataaccatc gaatggctgt tttctatcgc accacaacgc ggtggatgtc
     2161 atcgcgtcga tgttaattct ttcactcacc tgtagtggcg tcccttccaa cggagatata
     2221 acatgcaaaa agggggaccc ttctttggat gaatagtaac cgcgccgcgc tttctcgcgc
     2281 accaaatgcg taatcgacaa atcgggggaa ttaaatttgt gcatcgtgaa cgtataaaga
     2341 agcacgacat cgataccgaa agttttttcg tcttggtttg cagcaagcca cgcaaacgct
     2401 ttcgcggcca aaatcgccgc ataatttttg cgccccactg tttttgtatt gaagggagtt
     2461 ttggctgcaa caccaaaagt caaaaccaaa aacaggagga gcgcgcgttt catggggtta
     2521 gttcggtatc aattttgacc agctaaaacc atcacacccc ccggtggctg ggtcggtttt
     2581 atccgaggtg tctgcaatcg ccattgctgt gacttgagtt gtactattaa aaggagacag
     2641 agtacaagta aagtagtcgc tcgctttttt tgtcaaaata actttctggt attttgaggg
     2701 gttgtacgca tcgtttgagg gtttttgaat gatgaagtaa ctatcgattg cattgtaaag
     2761 cacaatggtg tcggcatcgc cccaagggct cgaccataaa attgcgccga tactaaaact
     2821 gttaccccaa tcgtctttcc aattacccat aacgggattc acaaccggcg taagtttcga
     2881 ccagctaaag ccgctacaac ctttcgtggc tgggtcggtt ttatcggttg tatccgtaat
     2941 cgcttccgca gccgcttgag ttgcactatc gaaaggcgac aacgtgcagg tataaagcga
     3001 gccgtcgttg ttggggatat aaaccacttt ttggtattta ttcgcgttgt acgcatcgct
     3061 cgccgacttt tgaaggatga tataccgcag aaccccatcg attttaacga cactgtcggt
     3121 ttggtcggtc caactatctt ttaactgcca agctgtggcg gtgagtacgt gctgaccgtt
     3181 gtacgtatct ttgaacgacc ctgagagacg gcttgacaaa tagtgactca acgcatcgac
     3241 ttcagaaaca agaggtgttg ctgttccgcg tgcaagcaat accacagctt gcaccaaagt
     3301 gtccgcttcg aatgccgccg tcgctttcga taaatcgatc acagaagcag caagcagatt
     3361 gcgcacttct tcctttatta caataccgtt agcgttgttg ccgtcgctgt cgagggtttg
     3421 taaaagacgc aaagtgtttt ttactttttg attcgctaag ccatcggccg caaagtcttc
     3481 gggggtaact ataccagcac cttttgcact gccgagaatc gttttaccta atgaaaattc
     3541 gacattttca ccggcaatat acttgaaata accgtcactg tcggtgacac cgctttgcgt
     3601 tgctgttttg taagaaagcc ccgtaaccgg tgcatcgatg aaacgtcctt tttcaacggt
     3661 aactgccgcc cccatcgact gcccctgtgg aatactctca ttcgcacacg cgacaaaagt
     3721 gaggctgaat gcccaagcga gataccgcca ttttttagaa atttttttca tggaaaagct
     3781 cctgtaaaaa agagcccgcg atttgagcag aaaatattta ctcaaatgtt ttcggtacac
     3841 cgccgtgtcc tcgggtggtg cacacaagag ttccagactt agggggttgc gtcgagggtt
     3901 tgcgttttca ttcgcacgtt acgcgaatcg tccctttggc aacccacaac actgttgcgg
     3961 gtcagcaccg gattctaacc ggatttcctc tttgggaaaa tttccctaag gcaaatctat
     4021 gcagcgcgat agcgcgtaaa ttgtattgaa gatttacttc gttaaagtct taccccaaaa
     4081 tacaacaaga aaaattattc caaccacaat gcgataaatg ccgaacgcgg taaatccgcg
     4141 ttttgaaata aacgcaacaa aaaagcgcac agccaaaagc gccgttgcaa acgccaccag
     4201 tgcaccgatg acgagcacgt cgagatgggc agcgttgagc gtctcccgat ttttatagag
     4261 atcgtacgcg gtcgcgacga ccatcgtggg gactgcgaga agaaaagaaa actcgaccgc
     4321 acgcacgcga cttaatcccg cgacaagacc gccaccaatc gtggctgccg cccgcgaaac
     4381 gccagggatc atcgagatgg tttggaaaag accaataata aacgccgtcc tgggggtaat
     4441 ttggatttca tgcgttttat gtttctcgtc gcgattttgc gccagcttat caaaaaagag
     4501 taacaccaca ccgcccaaaa taagcgcaat cgacaccgaa agcggattaa aaagaagtgt
     4561 tttgatgtat ttgtaaaaga gcaacccgca cacacctgtg ggtaggaacg caatgaataa
     4621 aataaaataa agacgcggct ctttgaggaa gcggcgtata tagagaagag caacggcggc
     4681 gatggcgccg agttggatgg caatctcaaa agttttaaca aattcggggt cttggatttg
     4741 catcatcgaa tcggccagaa tcatgtggcc ggtggacgac atgggtaaaa actcggtgag
     4801 gccttcgacg atgccgagga taacggcttg cagggttgtc attcccgttg gttcaaaaat
     4861 tttgaacatc gggtcaacca tcatcaaaat ttatacgaca gagcctgtgt gattatggca
     4921 tggttgccta tatcttgaaa tcgtcgccta aatagatctc gcgcgctttt ttgtcgttaa
     4981 tgaggatatc cgccgtacct tcgatgagga cttgcccttg aaaaatgata taagcgcggt
     5041 cggtgagttt caacgtctcg cgcacgttgt gatcggtgat gaggataccc aagccacgct
     5101 cttgtagttg ccacaccacg cgttggattt ctttcactgc aatggggtcg acaccggcaa
     5161 acggctcgtc gagtagtaga aatttgggat cggaagcgag cgcacgcgca atttcagcac
     5221 ggcgcctttc accccccgag agagtaatcc cgttttgatt ttgaacgtgc tcaatctcaa
     5281 gttctttgag aagcttatct gtttcttcct taattaggct cttttcaaaa cggcgcagtt
     5341 caaaaatcgc ttcgatgtt
//
LOCUS       DAOMPD010000151         5351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6479_length_5351_cov_2.445053, whole genome shotgun sequence.
ACCESSION   DAOMPD010000151 DAOMPD010000000
VERSION     DAOMPD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6479_length_5351_cov_2.445053"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1382)
                     /locus_tag="PLY93_06545"
     CDS             complement(237..1382)
                     /locus_tag="PLY93_06545"
                     /inference="COORDINATES: protein motif:HMM:NF027399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69172.1"
                     /translation="MPKLLDAAKAGQAKFVLQFGGQGAPYLKEMQKLYAEASMKPYFE
                     IAISAIEAAAKMVEGTAAHPEKVDFRKWLTEADSAPSDDFMSIAGISLGLIQATQFAH
                     YEHLNQQGFDRKTMLAHTTATTGHSQGLIAASFAALGLEGQAYNDAVYKYIQYLFLMG
                     VRAQEVFPHIFATADENAKSEALGAKAPAPMVAVLGDTHATIENWVKDFNNGAKKLFV
                     SLYNTPANRIISGTRSDLIAFHEKHKADFEAKQIKYVYLRSTCPFHSELMTPILEKFR
                     PDLAKICFDFKGSDLKVPVYSFWDGSNLQNEADLGMRMTDDLMVKTLYWEKAMLPVKN
                     DKSITHILDFGPGKTSQRLSMDTLAGLGHEVPVYAIAFAKDLKTVLE"
     gene            1519..>1748
                     /locus_tag="PLY93_06550"
     CDS             1519..>1748
                     /locus_tag="PLY93_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgL"
                     /protein_id="HRP69173.1"
                     /translation="MRVTNLMQNNTMISTLNRHQQAFDKTERELSTGSKIQLPSSDPV
                     AAANQMLYRTRLTELEQYDRNIGEAKDRLNVM"
     assembly_gap    1749..1758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1759..2776
                     /locus_tag="PLY93_06555"
     CDS             <1759..2776
                     /locus_tag="PLY93_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HRP69174.1"
                     /translation="LDRVGDIFQRMRYLTVQAANGTNSGFELKEAIAKEINQHLLALI
                     DIANSKDATGRSLFGGSVIERDPFLPVYSHLQTAGIDQGNAVTGVQYQGDIQVQNREI
                     ERNEQMDITLPGNRVFWGTNTSIASNRDSAAYVAQGDQLIAVNGVRIQVSAGDTINDI
                     IDKINTAPVEVRASKGSQDDLILTSKSPHQIWLEDLEGGTVLQDLGLIDGANPEPPNN
                     FATTATVSGLSIFETVIMLRDDLIKGDTKLIGGRDLEALDSALENILRYRSEVGARVN
                     RLEQHEKRVAWDTTYTKELLAKNESVDVTQSIIDLKWLEAVHSYALRVGAGIIKQTLM
                     DFLR"
     gene            2904..3953
                     /locus_tag="PLY93_06560"
     CDS             2904..3953
                     /locus_tag="PLY93_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRP69175.1"
                     /translation="MKEKIEKLLKRVQNPLTKKMIVDEKLIVSISGDTKPVVEIKSGG
                     DEKWRQGIESQLRIMGNRDGIKTDDYIIKWSDIKPEVAKQGPVSRPQLSIPGVKHVIA
                     VGSGKGGVGKSTVSVNIASTLQQMGHKVGIMDADIYGPSVGKMFGIDGRQQMNVANNH
                     ITPIEKYGLKVMSFSFLIAEGEPVIWRGPMLGKAMQQFLYDTDWGELDYLIIDLPPGT
                     GDTQLSLAQLVQTDGAVIVTTPQTVALLDAGRAVAMFEKVNIPILGVVENMSEFICTH
                     CGKPNHIFAKGGGSRLAASSKSILLGQVPLIEDAMSAAEKGEIPVYQNKNKKLEPLVD
                     AFTHIVQNLASVLTR"
     gene            3976..4497
                     /gene="dcd"
                     /locus_tag="PLY93_06565"
     CDS             3976..4497
                     /gene="dcd"
                     /locus_tag="PLY93_06565"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRP69176.1"
                     /translation="MILSGKEIEKRLGKDIIIDPYDPKKLNPNSYNLTLHDELLVYKE
                     RVLDMKKPNEAQLLKIPPEGFVIEPGRLYLGRTHEFTETKNIVPMLEGRSSVGRLGLF
                     VHITAGFGDIGFKGYWTLEISAIQPVRVYAGVEICQIFYHTILGDFTEYTSGKYQGNT
                     GIQPSLIYKDFEK"
     gene            complement(4476..>5351)
                     /locus_tag="PLY93_06570"
     CDS             complement(4476..>5351)
                     /locus_tag="PLY93_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69177.1"
                     /translation="GMADIQNKILRTIGDPVARFTEDGLRPIRGCRIAAKNAFTIEEK
                     TRLAMFACRHVTAKVAPERFYDEWRKTLHLKDKAAFWQNILNAGIVGAFLPTIESFFS
                     AERSGIFLREINLIQPRTMAAYAASIFYLLRITDNSLQKTTLLNTKFPTAQLKLALSL
                     LASPLLIIDATTSRLEFKKNLMRITKLERVHHVRFAIAMRLAAMREEGAIPQAQKDFE
                     THVRALYREIRKSREAIDMGALFINGVDIQSLGVTGRNVGVVLQHLLNKVIETPALNN
                     RDRLLEEARHFSKSL"
BASE COUNT         1435 a         1268 c         1316 g         1322 t
ORIGIN      
        1 atattaaata ccaaaaagcg gtcaaatttt ctcacttttg gaaaaaaaag tgcaattttt
       61 ggtaaaaatt atattttttt tgcagagaac actcgtgtgg atagttatcc cctaatgtga
      121 cataatcgaa ttctggacaa gaatagttat cgcgcgcgcg ataaatcagt cagagtaata
      181 gtttcacacc ctgctgggtg ttaatgataa aagtctcgca gttatctcac aacgctctat
      241 tctaaaacag tcttcaagtc ttttgcaaat gcaatcgcgt acacaggcac ttcgtggcct
      301 aagccagcga gggtgtccat actgaggcgc tggctggttt tgcccggacc aaaatccaaa
      361 atgtgcgtga tcgacttgtc gttcttcact ggcagcattg ctttttccca gtagagagtt
      421 ttcaccatga ggtcgtcggt catccgcatc cccaggtcgg cttcgttttg taaatttgag
      481 ccatcccaaa aagaatagac gggcaccttg aggtcagaac ctttgaaatc gaaacaaatt
      541 ttcgctaaat cgggtctgaa tttctccaga atcggcgtca tgagttctga gtgaaacggg
      601 caagttgaac gtaagtacac gtacttaatt tgctttgcct caaaatcggc tttatgtttt
      661 tcatggaaag caatgaggtc gctgcgtgtg ccggaaatga tgcggtttgc gggggtgttg
      721 tacaaactca caaaaagttt tttcgcaccg ttgttgaaat cctttaccca gttttcaatc
      781 gttgcatgcg tgtcgcccaa caccgctacc atgggagcgg gagcctttgc gcccaacgct
      841 tcacttttcg cgttttcgtc ggcagtggca aaaatatgtg gaaacacttc ttgcgcacgc
      901 acacccatga ggaaaaggta ttgaatatac ttatacactg cgtcgttgta cgcttggcct
      961 tctaaaccga gcgcggcaaa actggcggca atgagccctt ggctgtggcc tgtagtcgcg
     1021 gtggtgtgag cgagcatcgt tttgcgatca aaaccttgtt ggttgaggtg ctcgtagtgc
     1081 gcaaactggg tggcttgaat gagtcccaac gagatgcccg caatcgacat aaaatcatcc
     1141 gacggcgcag agtcggcttc ggtgagccac ttgcgaaaat ccactttttc gggatgagca
     1201 gcggtacctt cgaccatttt agccgccgct tcaatcgctg aaatggcaat ttcaaaatac
     1261 ggtttcatcg acgcttcggc gtagagcttt tgcatttctt tgaggtacgg tgcaccttgg
     1321 ccaccgaatt ggagtacaaa tttagcttgg cctgctttgg ccgcatcgag taatttaggc
     1381 attcgcgcgt ttactgaaaa agaaacagcg tgtcgagaaa aatgccttgt ttgaagtagg
     1441 ggaacatcga ttgtatatcc taatttagta ctcacctaat ttatctaaat taccgaatat
     1501 agaatggggt actttatgat tcgcgtcact aatttaatgc aaaataatac gatgatttca
     1561 acgctcaatc gccatcaaca ggcgttcgat aaaaccgagc gggagttgtc gacaggatct
     1621 aaaatacaat taccttcgtc cgacccagtg gcggcggcaa accaaatgct ttaccgcaca
     1681 cgtctcaccg agctcgaaca gtacgaccgc aacattggcg aagcgaaaga tcgcttgaat
     1741 gttatggann nnnnnnnngc tcgaccgcgt tggtgatatt tttcaacgaa tgcgctacct
     1801 cactgtgcaa gcggcgaacg gcactaacag tggttttgaa cttaaagaag cgatagctaa
     1861 agaaatcaac caacacttac ttgcgctcat cgacatcgcc aatagcaaag acgctactgg
     1921 tcgctcgctt tttggtggca gcgtgattga acgcgatcct tttttgccag tgtactccca
     1981 tttgcaaacg gcggggattg accaagggaa tgccgtgact ggcgtgcagt atcaagggga
     2041 catccaagta cagaaccgcg aaattgagcg caacgaacag atggatatta cgctaccggg
     2101 caatcgtgtt ttttggggaa ccaacacatc gattgcatcg aacagagatt cggcagcgta
     2161 cgttgcacag ggagaccaac ttattgcggt taatggcgtg cgtatccaag tttcagcggg
     2221 cgatacaatt aacgacatca tcgacaaaat caataccgca ccggtggaag tccgtgcgag
     2281 caaaggctca caagacgatt taatcttaac aagcaaatcg ccacaccaaa tttggcttga
     2341 agacttggaa ggtggtacgg tgctgcaaga cttagggctc atcgacggcg ccaaccccga
     2401 accacctaac aactttgcaa cgacggcaac ggtttcgggg ctttccattt ttgagacagt
     2461 gatcatgctg cgtgacgacc tcatcaaggg tgacaccaaa ctcatcggcg gacgcgacct
     2521 cgaagcgttg gatagcgcgt tagaaaatat tttgcgctac cggtctgaag ttggtgcacg
     2581 cgtgaaccgt ttggagcaac acgaaaaacg cgttgcgtgg gacaccacct acacgaaaga
     2641 gctacttgcc aaaaacgaga gcgtggatgt aacgcaatcg attatcgatt tgaaatggct
     2701 tgaagcggtg cattcgtatg cactgcgtgt cggtgcgggg attatcaaac aaacgttgat
     2761 ggattttcta cgatagaagt atagactgtc tgccgtctcg atggggtacg cccaataata
     2821 gacgttttgt tccgctttcc gctttgcgcc atgtctcaat ctgtcgtttt gtcttcctta
     2881 atagataatt gagacttata ctgatgaaag aaaaaattga aaaattgcta aagcgggtgc
     2941 agaaccctct caccaaaaaa atgattgtcg atgaaaagct catcgtctct atttctggcg
     3001 acaccaaacc ggttgtcgaa attaaatccg gcggcgacga aaaatggcgc caaggcattg
     3061 aaagccaatt acgcatcatg ggcaaccgtg acggcatcaa aaccgacgat tacattatca
     3121 aatggagcga catcaaaccc gaggtcgcca agcaagggcc ggtttcacgc ccgcagttga
     3181 gtatccccgg cgttaaacac gtcatcgctg tgggctcggg caaaggcggt gtgggtaaat
     3241 cgacggtgtc ggtaaatatt gcttccactt tgcaacaaat gggacacaag gtcggtatta
     3301 tggacgccga catttacggg ccgtcagtgg gtaaaatgtt cggcattgac gggcgccaac
     3361 aaatgaacgt ggcgaataac cacatcactc ccatcgaaaa atatggtctt aaagtcatga
     3421 gtttttcttt cctcatcgcc gaaggggaac cggtaatttg gcgcggcccg atgctcggca
     3481 aagcgatgca gcaatttcta tacgacaccg actggggcga actcgattat ctcatcatcg
     3541 acttaccccc cggtactggc gacacacagc tttccctcgc gcagctcgtg caaaccgacg
     3601 gcgcggtgat tgtcaccact ccgcagaccg tcgctctatt ggatgcaggc cgtgcagtgg
     3661 caatgtttga aaaggtgaac atccccattt taggcgtggt agagaacatg agcgagttca
     3721 tctgcacgca ttgcggcaag cccaaccata ttttcgccaa aggtggcggc tctcgtttgg
     3781 cggccagctc aaagagtatt ttactcggcc aagtgccgct catcgaagat gcgatgagcg
     3841 ccgccgaaaa aggcgaaatt cctgtgtatc aaaataaaaa caagaagctc gaaccgctgg
     3901 tcgatgcgtt cacgcatatt gtgcagaacc tcgcttcggt gttgacgcgc taagaaaaag
     3961 attgcgagag aaagcatgat cctctcggga aaagaaattg aaaaacggtt ggggaaagat
     4021 atcattatcg acccttacga ccccaaaaaa ctcaatccta attcgtataa tctcacccta
     4081 cacgacgaac tactcgtgta taaagagcgc gtgctcgaca tgaaaaaacc caacgaggcg
     4141 cagcttttaa aaatccctcc cgagggtttt gtaattgaac caggcaggct ctatttaggc
     4201 cgcacgcatg aattcaccga aactaaaaac attgtgccga tgctcgaagg gcgcagcagc
     4261 gttgggaggt tgggactctt cgtccacatc accgcagggt tcggcgacat cggcttcaaa
     4321 ggctattgga cgttagaaat ttcggcaatc cagccggtgc gtgtgtacgc gggggttgaa
     4381 atttgccaaa ttttttacca cactatcctg ggcgatttta ccgagtacac cagcggcaaa
     4441 taccaaggca acaccggcat ccagccgagc ctcatttaca aagattttga aaagtgacgc
     4501 gcttcttcaa gtagcctgtc acggttgttg agcgcgggtg tttcgatcac tttattcaag
     4561 aggtgttgta gcacgacgcc cacgttgcgg ccagtgacac ccaaactttg aatatccaca
     4621 ccgttgatga aaagcgcacc catgtcaatc gcctcccgcg actttcgtat ttctcggtaa
     4681 agcgcacgga catgcgtttc aaaatctttt tgtgcttgag gtatcgctcc ttcttcgcgc
     4741 atggcagcaa ggcgcatagc gatggcaaaa cgaacgtgat gcacgcgctc taattttgta
     4801 atacgcatta aatttttttt gaactcaagc cggcttgttg tggcatcgat tattaaaagc
     4861 ggcgacgcta aaagcgaaag cgcgagctta agttgcgctg tgggaaattt tgtgttgagg
     4921 agagttgtct tttgcaacga attgtcggtg atgcgcaaaa gataaaaaat cgacgctgcg
     4981 tacgccgcca tggtgcgcgg ttggattaag ttaatttccc gtaaaaaaat accgctgcgt
     5041 tcggcggaaa aaaaactttc tatcgtgggt aaaaatgcgc ccacaatccc tgcgtttaaa
     5101 atgttttgcc aaaacgccgc cttgtctttg agatgcagcg ttttgcgcca ttcgtcataa
     5161 aaacgctcgg gggcaacttt agcggtgaca tgcctacatg caaacatcgc gaggcgggtc
     5221 ttctcttcaa tggtgaaagc atttttcgcg gcgatacggc aacctcgaat cggtcgtaag
     5281 ccatcttcgg taaagcgagc caccggatcg ccaattgtgc gtagaatttt attttggata
     5341 tcggccatac c
//
LOCUS       DAOMPD010000152         5314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6530_length_5314_cov_2.735881, whole genome shotgun sequence.
ACCESSION   DAOMPD010000152 DAOMPD010000000
VERSION     DAOMPD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6530_length_5314_cov_2.735881"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..>442
                     /locus_tag="PLY93_06575"
     CDS             82..>442
                     /locus_tag="PLY93_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69178.1"
                     /translation="MISILLVQYRTLYAFLSIFTFTTSYHPTDYNFDIDDNSKSTPSN
                     KTSANDSRVKPPVSSKKNSVSSDDVFALFKGEQKVTIVSKKEERVQDAPATIYVITDK
                     EIQERGYLFLHDALRDVP"
     assembly_gap    443..472
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <473..2640
                     /locus_tag="PLY93_06580"
     CDS             <473..2640
                     /locus_tag="PLY93_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP69179.1"
                     /translation="GMDTAENNRTLIYIDGVPTNNPSQGVSYMSYQFALHNVKRIEIL
                     WGPASALYGANAFAGIINIVTKNADDMEAAGQQGHFSAGTLIPKMRQSATPAGIYLDF
                     MIGGKMWEDKDAARITVSGHYIRSDTGLDYARHHSPAPGAAANTETYIPTHVGAIPIL
                     GNYNAQIRFDYKNLTIGARAWRMWTRQGGFATYNWTAYERMFWGFQGQDVYAKWNQKI
                     TPSLSYFSQVVFRASQVPSGEYGQHGYDADRSGVMTGGRLLWYKRDDYAASWDNQITT
                     DWALGTSSVGIFAEYARVSNWNTNDDRYKKWNGTSFVQRYPNEFRGNFDANGNLIGPY
                     YEGQPPQDDKPEVFYNQYNLAAYLQHNKDILENLSLTLGARVDVLFLHGAEGAEILGT
                     APTDLGACPTCIDPANPRVFATPADAAAAGANLQIGDGYFIGKPVNQTTVSLNPRIGF
                     VYNPTPNQTIKILHGWAFRNPTVRERYSFTSSRIAVGNDLKPEQIKTTEVGYSIRPIP
                     ILRAELDGFWSEVSDVIQLSSTPWLRPNKTTENPYAQFQNVGAARLLGFELKTDVALV
                     RTDPLSVLVFANYSFQHNRYTDIIAGAKSSLSHDLSEPGDNKDSNMMPRVSSHKFNVG
                     FTFYIQKFFSLSPILHFVGARPNVITNPFEEVPAYTLFNLSAAYRNPQNNWEASLFIF
                     NFFDQEINDPGTRDASGKYYASLRPIERLTVWARLTYRL"
     gene            2637..3977
                     /locus_tag="PLY93_06585"
     CDS             2637..3977
                     /locus_tag="PLY93_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRP69180.1"
                     /translation="MNYSKSIACCLFGFLFFTTHTIAAEKEVRIVYEKGARIQEITLD
                     GIQSRLTHTKAFILGDDAAFENCDSGSVGAVIAIGKPSLSKAIAACKNTPIVFSLVSA
                     PRLGEIKSHPNVTGISFDLSFRLFLTELQKIMKSGSRIGFIYSSPENEFLATEIDYVE
                     SEFNFTGVRLRTENRDELGAKVEQLIEKDKVSALWVLPDPLYNQAIFRKLAELCAEKG
                     VLLVTNFEVLVSEAGAAIALAPSYFDTGVQTAEIVQKILAGTKPIDISYQRPHTSGVY
                     LNLPLFERLKINLPNDLRYKEKVTTLLNEAQDLQKAGQAQQALSKFREVLRYDKKNTT
                     AHYFVNAIESHNNYNTALQRINAGNKMGAIGLLIASSPFLPEARARLQSLRGELRGQV
                     PGIFHRGVAQFQRKKYRDTINTMNLVLMIDPGHHEAQLYKEKAEKRAKAVSAIR"
     gene            3977..>5314
                     /locus_tag="PLY93_06590"
     CDS             3977..>5314
                     /locus_tag="PLY93_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRP69181.1"
                     /translation="MLNRISVKLALTVLVVSVVAAIPLSFYILAKQEKEKIDSTIAIG
                     RGNTKLLGKTMLDVFLKTGGEPASMHIDAEEAISTYRDQFELGLVFGQTLMMNKKHRG
                     LIMATIDKDAPVAGEKNANAKPAVKAQTVRTEAEITNFLNYENPHTGPCFNNAKSKCY
                     NFAFVAKYEKAPVVLSVLSISEDHVLRPIQQLRTLIYISTGVAVILALLLGIGVSRLI
                     TGPISALVEGVKRYSQGDLDYKVDIRVKDELKTLGTAFNDMADQIATKIKEIEDHRDN
                     LELKVEERTSELRTALVEVSKLKEQQDADYYLTTFLFKPLCINRNSSEFIKTEFFLKQ
                     KKEFSFRDKKGEIGGDICITANILFREESGLVNRYLFATNADAMGKSIQGAGGAIVYG
                     TAINTILASSTTNDRVLETSPREWLMRMYRELHNIFLSFDGSMLVSGRFMLLNE"
BASE COUNT         1526 a         1294 c         1176 g         1288 t
ORIGIN      
        1 agcgaccagc aaaattggct gcatagctgt caccgatgtc tcggggttct acattaataa
       61 aatatttgac atgtcattac catgattagt atacttcttg tgcagtaccg aacactctat
      121 gcttttttat caatcttcac tttcacaact tcttatcacc ccaccgacta caatttcgac
      181 atcgacgaca acagtaagtc cacaccctca aacaaaacat ccgcaaacga ttcacgggtg
      241 aagccacccg tctcttctaa aaaaaattct gtgtcgagcg acgacgtttt tgctcttttt
      301 aagggcgagc aaaaagttac catcgtgtca aaaaaagaag agcgggtaca agacgcgcca
      361 gcgaccattt atgtcatcac cgacaaagaa attcaagaac gcggctatct gtttttacac
      421 gacgccctgc gcgacgtccc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggggatg
      481 gacaccgctg aaaacaaccg gacattgata tacatcgatg gagtccccac caacaaccca
      541 tcgcagggcg tgagctatat gtcgtaccag tttgcattac ataacgttaa acgaattgag
      601 attctctggg ggcctgcgtc ggcgctttat ggagccaacg ctttcgcagg gattatcaac
      661 atcgtaacca aaaatgcaga cgatatggaa gctgcagggc aacaaggtca ttttagtgca
      721 ggtacactca tacccaaaat gaggcaatct gccactcctg ctggcatcta tcttgacttc
      781 atgattggtg ggaaaatgtg ggaagataaa gatgctgcac gaataaccgt ttcagggcat
      841 tatatacgtt cggatacagg ccttgactac gcgcggcatc actcacccgc cccgggagct
      901 gctgcaaaca cagagacgta catccccact catgtcggag caatccccat tttgggtaac
      961 tataatgcgc aaatacgatt cgactataaa aatttaacaa tcggggcgcg tgcttggcgt
     1021 atgtggacac gacaaggagg gttcgcaacc tacaactgga ctgcgtacga acgaatgttc
     1081 tggggattcc aaggacaaga cgtttacgca aagtggaacc aaaaaatcac tccatcgctt
     1141 tcttactttt cgcaagtcgt ttttcgagca agtcaagtgc cttcaggcga atacggccaa
     1201 cacggttatg atgccgacag aagcggcgta atgacaggag gaaggttatt gtggtataag
     1261 cgcgacgact atgccgcgtc gtgggataac caaattacga cagattgggc tttagggaca
     1321 agtagcgtag gtattttcgc agaatacgcc agagtatcca attggaatac caacgatgat
     1381 cgatacaaaa aatggaatgg aacaagcttt gtgcaacgtt acccgaatga atttcgaggc
     1441 aatttcgatg caaacggtaa cttgattgga ccttactacg aagggcaacc accccaagac
     1501 gacaaacctg aagtttttta caatcaatac aatctcgccg cgtatttgca gcacaataaa
     1561 gatattcttg aaaatctttc tctcaccctg ggcgctcgtg tcgacgtact ttttctccat
     1621 ggcgccgaag gcgctgaaat actgggtact gctccaaccg acttaggcgc ttgcccaact
     1681 tgtatcgacc ctgcaaaccc acgagttttt gcgacaccag cagatgctgc ggcagcggga
     1741 gcaaacctgc aaatcggcga cgggtatttc atcggtaaac cggtaaacca aaccactgtg
     1801 tcgctcaacc cacgcattgg ttttgtgtat aatccaactc cgaatcaaac gataaaaatt
     1861 ttgcatggtt gggcgtttag aaatccaacg gtgcgagaac gttattcgtt cacctcaagc
     1921 cgaattgccg tgggtaacga cttaaaacca gagcagatta aaacaaccga ggtgggttat
     1981 tcaatccgcc ctataccaat actgcgcgct gagctcgacg gtttttggtc ggaggtgagc
     2041 gatgttatac agctatcgag cactccatgg cttcgcccta ataaaacgac agaaaaccct
     2101 tatgcgcagt ttcaaaatgt cggtgcggca cgcctcttgg ggtttgaatt aaaaaccgat
     2161 gtcgctcttg tgcgtaccga ccctctcagc gttctagtct ttgcgaatta ttcttttcaa
     2221 cataatcgtt acaccgacat tattgcaggc gcaaaaagtt ctttaagcca tgatttatcc
     2281 gaaccgggcg acaataaaga ctcgaatatg atgccgcgcg tctcatcgca caaatttaat
     2341 gttggattca ctttttatat ccaaaaattc tttagtctat cgccaattct acacttcgtg
     2401 ggtgcgcgac caaacgtgat tacaaatcct ttcgaagaag ttcctgcgta cactttattc
     2461 aatttaagtg cagcgtaccg caacccacag aacaattggg aagcatcatt gtttatattc
     2521 aacttcttcg atcaagagat taacgatccc ggtacacgcg acgcctcagg caagtactat
     2581 gcatcgttac gtcccatcga acgactcacc gtgtgggcaa ggttgacgta cagattatga
     2641 attactcaaa gtccatcgct tgctgccttt tcggatttct tttttttacc acccacacaa
     2701 tagcggcaga aaaagaggtg cgcattgttt atgagaaagg tgcgcgcatt caagagatta
     2761 ctctcgacgg tatacaaagt cgcttgacgc ataccaaagc atttatcctg ggcgacgatg
     2821 ctgcgttcga aaattgcgac agtggcagcg tgggcgcagt gattgccatc ggtaaaccgt
     2881 cgctcagcaa agcgatagcc gcgtgtaaaa atacgccgat cgttttttcg ctggtttctg
     2941 ctccgcgttt aggcgagatt aaaagtcatc ccaacgttac aggcattagt tttgacttat
     3001 cgtttcggct ttttttgaca gagttacaaa aaattatgaa atccggatcg cgtatcggtt
     3061 ttatctacag ctcacccgag aatgaatttc tcgcgacaga aatcgactat gtagaaagcg
     3121 aatttaattt caccggtgtg cgcttgcgca cagaaaaccg cgacgagttg ggggcaaaag
     3181 tcgaacagct cattgaaaaa gataaagtga gcgcgctttg ggttttaccc gatcctctct
     3241 acaaccaagc catcttccgt aagctcgctg agttgtgcgc cgaaaaaggc gttctccttg
     3301 tcacgaattt cgaggtactc gtgagcgaag caggcgcggc gattgcgctc gcccccagtt
     3361 acttcgatac aggcgttcaa acggcagaga ttgtacaaaa aattttagcc ggcacaaaac
     3421 ctatagatat ttcgtaccag cggccacaca cctcgggtgt gtatctcaat ttaccgcttt
     3481 ttgagagact taaaatcaat ctgccgaacg atttgcggta taaagaaaaa gtcacaacac
     3541 tgctcaatga agcgcaagat ttgcaaaaag cagggcaagc gcaacaagcg ttgtcgaaat
     3601 tccgtgaagt cttgcgctac gacaaaaaaa ataccaccgc gcattatttt gtcaacgcga
     3661 ttgaatcgca caacaattac aacactgcgc ttcaacgcat caatgccggt aacaaaatgg
     3721 gcgctattgg ccttttaatt gcatcgagtc cttttttgcc cgaagcgcgt gcccggcttc
     3781 aatcgttacg cggcgaattg cgcggtcaag tgccgggaat tttccaccga ggggtcgcgc
     3841 aatttcagag aaaaaaatac cgcgacacca tcaacaccat gaacttggtt cttatgatcg
     3901 accccggtca ccacgaagca caattatata aggagaaagc ggaaaagcgc gctaaagcgg
     3961 tttctgcgat acgctaatgt taaacagaat ttcagtcaaa ctcgccctca ccgtactcgt
     4021 tgtctcggtg gtggcggcaa ttcccctttc attttacatc ctcgccaagc aagaaaaaga
     4081 aaaaatcgat tccaccattg ccattgggcg cggtaacaca aagctcttag gtaaaaccat
     4141 gctcgatgtt tttcttaaaa ccggcggaga gcccgcgtcg atgcacatcg atgccgaaga
     4201 agcgataagc acctaccgcg accaattcga gttaggtctt gtgtttggac aaacactcat
     4261 gatgaataaa aaacaccgtg gtctcatcat ggcgacgatc gacaaagacg cacctgttgc
     4321 aggtgaaaaa aacgctaacg ctaaaccagc ggtaaaagcg caaacggtgc gcaccgaagc
     4381 agaaattacg aatttcctca actacgaaaa tccacacacc ggtccctgct ttaataatgc
     4441 taaaagtaaa tgctacaatt ttgcgtttgt ggcaaaatat gaaaaagcgc ctgtggtgtt
     4501 gtcggtgcta tccatttcag aagaccatgt tttacgcccc atacaacaac tgcgtactct
     4561 tatttatatc agcacggggg tcgcggttat tcttgcgctg cttttaggca ttggcgtatc
     4621 gcggttaatc accgggccaa tatctgcgct cgtcgaaggc gtgaagcgtt actcgcaagg
     4681 cgatctcgat tacaaagtcg acatccgcgt caaagacgaa ctcaaaacac tcgggactgc
     4741 tttcaacgac atggccgatc aaatcgcgac aaaaattaaa gagattgaag accaccgcga
     4801 caaccttgaa ctcaaagtag aagagcgcac gtctgagtta cgcaccgcat tggtcgaagt
     4861 ttcaaaactc aaagaacaac aagatgcgga ttactactta accacatttt tattcaagcc
     4921 gttatgcatt aacagaaact cttctgaatt tattaaaacc gaatttttct taaaacagaa
     4981 aaaagaattt tcgttccgcg ataaaaaggg agaaattgga ggcgatatat gtatcactgc
     5041 gaacattttg ttccgcgaag aaagtggttt agtgaaccgt tacctcttcg cgacgaacgc
     5101 cgatgcaatg ggaaaatcga ttcaaggcgc aggtggcgca attgtatacg gaaccgcgat
     5161 caacaccatt ctcgcctcgt cgacgacaaa cgaccgcgta cttgaaacca gcccccgcga
     5221 atggctcatg cgcatgtacc gagagctaca caatatattt ctctctttcg acggttccat
     5281 gctggtgtcg ggccggttta tgctactcaa cgag
//
LOCUS       DAOMPD010000153         5312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6531_length_5312_cov_2.629827, whole genome shotgun sequence.
ACCESSION   DAOMPD010000153 DAOMPD010000000
VERSION     DAOMPD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6531_length_5312_cov_2.629827"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1746)
                     /gene="mqo"
                     /locus_tag="PLY93_06595"
     CDS             complement(172..1746)
                     /gene="mqo"
                     /locus_tag="PLY93_06595"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF003605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HRP69182.1"
                     /translation="MILKKLASLKEKKLRQNKEEARMASPNKEWYDLRETPDIVLVGA
                     GIMSATLAMLLKELEPSLTIAVFERLDEAARESSDAWNNAGTGHSALCELNYTPEKKN
                     RKIDITKAVQIAAQFETSKQLWAYWVRKGYLKDPHDFIHRVPHMSFVWGEENVAYLKK
                     RQTALKQHPFFKSMRYSNNADEIATWAPLLIEGRDKTQKIAATRVELGVDMNFGNLTR
                     ALFQYLDNLAGITVYYEHEVRDIERDANGKWKMMVTNTRKKRRFHVESDFIFIGAGGG
                     ALTLLEKSGIKEAKGYGGFPVSGQWLRCKNRSVIEKHAAKVYGKAAVGSPPMSVPHLD
                     TRHIDSKKELLFGPYAGFSTKFLKKGSYFDLMKSLGFTNVIPMIAAGLNNFSLTKYLI
                     EQATQSHAQRMKALKEFMPEARDEDWELLVAGQRVQVIKADDEEGGVLEFGTEVIAAH
                     DSSLVALLGASPGASTSVSIALEILSTCFQSNMATPKWKKKIQTMIPTYGKKFVTDAA
                     LIEKTRRDTAKILKLN"
     gene            complement(1743..3314)
                     /locus_tag="PLY93_06600"
     CDS             complement(1743..3314)
                     /locus_tag="PLY93_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69183.1"
                     /translation="MLAQMKSLWLRYLNSGSRHFSTPTDLKLNRVLNLLFSLHYFAGG
                     FLIVTEIVVLIILLNRDFDKYIQYALPFFTLNVVHFVSVAIVIYAKNRWGNFQVSFIS
                     DAIYTAYCITLSVFLGETVAIHIILLSVLPVVFTVFNYGRWKEALLHAAFISVGIALS
                     LMSYRLIPPLYPLPPDIAQVSGYLAYAVGIILIFIYSTYNWKQVYDTEQQLEHEKNYT
                     HSLLNEAIPKLEMAEANYRYLIENSSDMIFQMNAEGQFLSMNKTSRDILSFLPEEMIG
                     KSLFDFIAEMTEGKEHMDNRFVHKHLQQLIDGKKIVSFKMTLRKKHMQELVVTQVTLH
                     KPDFSHTNEFLGKISRLEQDITRQFIEREKGRYSIGNNITYAEALSQKLAEKLWKYFD
                     SSTINTIRICLREILVNAIEHGNLAISFQDKSRIIAQHDYMEFLFERQKDSKYASRRV
                     HVDFLINQYALFFRVTDEGDGFDHRTFLERAARISREDMLEHGRGILMTRNAFDSVTY
                     NDKGNQVILTKQIPK"
     gene            3398..4948
                     /locus_tag="PLY93_06605"
     CDS             3398..4948
                     /locus_tag="PLY93_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRP69184.1"
                     /translation="MSKRDEWGGRLGIILAVTGSAVGLGNFLRFPGLAAKYGGGTFMI
                     PYLIAFLILGLPVAYVEWSLARYGGQRGFHSAPGIFSVLWPKKISPYLGVLGLIVPVG
                     IYMYYVFIEAWCLYYAWGFISGALQLGTDITQYKSFFESFTGSSANGVLFQSASPALI
                     ALVLCYLLNFFFVYRGIAGIERISRWGMPLLLVIALVVLVRVMTLGTPDAAYPERNVI
                     NGLGHLWNPPATGVLTSPANAQLWMDAAGQIFFSLSVGFGVIINYASYLSRKDDIMRS
                     ATTAAAGNEFAEVALGGLITVPAAFVFLGAAGIGSTFGLGFVSLPAVFAHMPFGQIFG
                     ALWFFLLFVAAITSSVSMIQPGMAFLEEGLHLKRKHSTLLLCGITLLGSLFVVYYSGN
                     LEALDTMDFWVGTFFIFVLALSQVLIGGWLFGVENIIKEARQGAHMRIPKFLPVMIKY
                     ITPAFLGVVFILWCIQKLPEQWTKIQTNPVTQISILFLLGVCILFLIMTHFAVKRWNK
                     TPPKGDFP"
     gene            5047..>5312
                     /locus_tag="PLY93_06610"
     CDS             5047..>5312
                     /locus_tag="PLY93_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRP69185.1"
                     /translation="MKFFFYAFVIFSSALFASPQKRRLIIWSIDGFAAAYLTNPEFQK
                     IKVWQRLLKRAQLFTPVETVIPALTYPAHTAMVTGHETANHRLYG"
BASE COUNT         1301 a         1230 c         1233 g         1548 t
ORIGIN      
        1 ataaaaacga cgccgcgttc aaggcattag gttttgccaa agacattgtc tcgctcaacg
       61 cccaagcgct catcttaaaa atcaccgagc aaggcgatgc ggtcgatgcc gcacaattct
      121 atcggtcgat gacaaaagca gcgcgctcgg tgatccactg ggcgcagaaa gttagttgag
      181 tttcaaaatt ttggcggtgt cgcgccgcgt tttctcaatg agtgcggcgt cggttacaaa
      241 tttttttccg tatgtgggga tcattgtttg aattttcttt ttccactttg gcgttgccat
      301 gttactttga aagcacgtcg ataaaatttc cagcgcgata gaaaccgagg tcgacgcgcc
      361 gggcgatgcg cctaaaagag cgacgagcga actgtcgtgc gcggcgataa cctcggtgcc
      421 aaactccaaa acgccgcctt cctcgtcgtc ggccttaatc acttgcacac gttggcctgc
      481 aacaagaagc tcccaatcct cgtcgcgcgc ctcgggcata aattctttga gtgctttcat
      541 ccgctgcgca tgcgattggg tcgcttgttc gatgaggtat ttcgtgagtg aaaaattatt
      601 aagccctgcg gcaatcatgg ggatgacatt cgtgaatccc aaagatttca tcaaatcaaa
      661 atacgaacct tttttcaaaa atttagtcga gaaaccagcg tatggcccga acaacaattc
      721 ttttttactg tcgatgtggc gtgtatctaa atgcggcacc gacatagggg gagagcccac
      781 cgccgctttc ccgtatactt tcgccgcgtg cttttcaatg acgctgcggt ttttgcagcg
      841 cagccactga ccgctcactg gaaagccgcc atagcctttc gcttctttga ttcccgattt
      901 ttctaaaagc gtcagtgcgc cgccgcccgc accgataaaa ataaagtcgc tctctacatg
      961 gaagcggcgc tttttccggg tatttgtcac catcatcttc catttgccgt tcgcgtcgcg
     1021 ttcgatgtcg cgcacttcat gttcgtaata tacggtaatc cccgccaggt tatctaaata
     1081 ctgaaataac gcgcgcgtca aattgccaaa attcatgtcg acgcctaact cgacacgggt
     1141 agcggcaatc ttttgtgttt tatcgcgccc ttcgattaag agcggcgccc acgttgcaat
     1201 ttcatccgcg ttgtttgaat atcgcatact tttgaaaaaa ggatgctgtt tcagcgctgt
     1261 ctgccgcttc tttaggtacg caacattctc ttcaccccaa acaaaactca tgtgcggtac
     1321 acggtgtata aaatcgtgcg ggtctttgag atagcctttg cgcacccagt atgcccaaag
     1381 ctgtttggat gtttcaaatt gcgcagcgat ttgcaccgct ttagtaatgt ctatttttct
     1441 gtttttcttt tcgggagtgt agttcaactc gcatagcgcc gagtggccgg taccggcatt
     1501 attccacgcg tccgagcttt cgcgcgccgc ttcatcgagc cgttcaaaaa cagcaatcgt
     1561 aagagagggt tcgagttctt ttaaaagcat cgctaaagtt gcactcatga tgcctgcccc
     1621 cacgagaacg atgtcggggg tctcacgcaa atcgtaccat tctttattgg gtgaagccat
     1681 acgcgcttct tctttatttt ggcgtagttt tttctctttg agcgaggcga gttttttgag
     1741 tatcattttg gtatctgctt agtcagtata acctgatttc ctttatcgtt gtaagtgaca
     1801 ctatcaaaag cgttgcgtgt cattaaaata cctcggccat gttcaagcat gtcttcgcgc
     1861 gatattcgag cggcacgttc gagaaaagta cgatggtcaa aaccatcgcc ttcgtcagtg
     1921 acgcggaaaa aaagcgcata ttggttgata aggaagtcaa cgtgaacgcg ccttgatgcg
     1981 tacttagaat ctttttggcg ttcaaaaaga aactccatat aatcgtgttg tgcgataatc
     2041 cttgatttat cttgaaaact aatcgctaaa ttcccatgct caatcgcgtt cacaaggatt
     2101 tctcgtaaac aaatgcgtat agtattgatg gtagatgaat cgaagtattt ccaaagtttt
     2161 tcagcaagtt tttggctcaa tgcttcggcg tatgtaatat tatttccaat gctatatctt
     2221 cctttttcac gctcaataaa ttgtcttgta atgtcttgct ctaaacgcga aatcttacct
     2281 aaaaattcat tcgtgtgaga aaaatcgggt ttgtggagcg ttacttgggt aaccaccagc
     2341 tcctgcatat gtttttttcg gagggtcatt ttgaaagaaa caattttttt cccatcaatt
     2401 aactgttgta aatgcttgtg aacgaagcgg ttgtccatgt gctctttgcc ctctgtcatt
     2461 tcagcgataa aatcaaagag agatttacca atcatttcct ccggcaaaaa gctcagtatg
     2521 tcacgcgagg ttttgttcat actcaaaaat tgtccctcgg cgttcatttg aaaaatcatg
     2581 tcgctggagt tttcgataag ataacggtag ttagcctcgg ccatttcaag tttaggaatg
     2641 gcttcgttca aaagcgagtg ggtgtaattt ttttcatgct ctaactgttg ttcagtatca
     2701 tacacctgtt tccaattata cgtcgaataa ataaaaataa ggattattcc taccgcatac
     2761 gcaagatagc ccgatacttg tgcaatatcc ggcggtaaag ggtatagcgg cggaattaac
     2821 cgatagctca tgagagaaag ggcaatccct acactaataa aagcggcgtg taagagagcc
     2881 tctttccacc gcccataatt aaatacagta aagacgacag gtagaacgct caaaagaata
     2941 atatggatgg caaccgtttc accgagaaac acagagaggg taatacaata cgctgtgtaa
     3001 atagcatcgc taataaaact gacttgaaaa tttccccaac gattttttgc atagatgacg
     3061 atagcaacag agacaaaatg taccacgttc aaagtaaaaa aaggtagtgc atactggata
     3121 tacttgtcga agtcccgatt gagaagaata ataagaacaa caatctcagt aacaattaaa
     3181 aacccacccg caaaatagtg caatgagaaa agcaggttta gcactcgatt caacttgagg
     3241 tcggtgggag tggagaagtg gcggctgccc gagttaaggt atcgtaacca caacgacttc
     3301 atttgagcca acatgcgttc tcgtcataaa ggaaattttt tacccatcgt aaagtcttta
     3361 gcttgaccgc ctcgccggaa aataaaaact taaaaagatg tcaaaaagag acgagtgggg
     3421 tggtcgcctc ggtattattc ttgcggtgac aggctctgcc gtggggttgg gaaatttttt
     3481 gcgcttccct gggcttgccg cgaagtatgg cggcggcaca ttcatgatcc cctatctcat
     3541 cgcttttctt attttgggtt tgcctgtggc gtacgttgag tggtcgcttg cgcgttacgg
     3601 tggtcaacgc ggcttccata gcgcgccggg aatttttagc gtgctatggc ctaaaaaaat
     3661 atccccctac ctcggcgtct tgggactcat tgtgccggtg gggatttaca tgtattatgt
     3721 ctttatcgaa gcatggtgcc tttattatgc gtggggattt atttcaggag cgttgcagtt
     3781 aggaactgat attactcaat acaaaagctt ttttgaaagt tttaccggtt cgtcggccaa
     3841 tggggtgtta ttccagtcgg cgtcccccgc tttgattgcg ctcgttttgt gttatctcct
     3901 caatttcttt tttgtttacc gcgggatagc cggcattgaa cgcattagcc gatgggggat
     3961 gccgttactc ctcgtcatcg ctctagtcgt tttagtacgg gtgatgacat taggcacacc
     4021 cgatgctgcg taccccgagc gtaacgtcat caacggtttg gggcacttat ggaacccacc
     4081 tgcaacggga gtgctgacat cgcccgccaa cgcgcaactc tggatggacg cggcggggca
     4141 aatttttttt tcactctcgg tgggttttgg cgttatcatt aactacgcca gctatttgag
     4201 ccgtaaagac gacatcatgc gctcggcgac aaccgctgcc gccggcaatg aatttgccga
     4261 ggtggcgttg ggtggactca tcactgtgcc tgcggcgttc gtctttttgg gggcggcggg
     4321 catcggcagt acgttcggtt tggggttcgt gtcgttgcca gcggtctttg cgcacatgcc
     4381 tttcggtcaa atttttggcg cgctgtggtt ttttcttctt tttgttgccg ccatcaccag
     4441 ctcggtttcg atgatccaac ccgggatggc ttttttagaa gaggggttac acttaaaacg
     4501 aaagcatagc actctcttgc tctgcggcat cacactttta ggttcgcttt ttgtcgttta
     4561 ctattcgggg aatttagaag cgctcgacac gatggatttt tgggtgggca cattttttat
     4621 ttttgtgctg gcgctctccc aagtcctcat cggtggttgg ctttttggcg tcgaaaacat
     4681 aatcaaagaa gcgcgccaag gggcgcacat gcgtatccct aaatttttgc cagtgatgat
     4741 taaatacatc acacccgcat ttttaggagt ggtgtttatt ttatggtgta tccaaaaact
     4801 ccccgagcaa tggactaaaa tacagacaaa ccccgttact caaatttcta ttttattttt
     4861 gttgggggtt tgtattctgt tcttaatcat gactcatttt gccgtcaaaa ggtggaataa
     4921 aactccaccg aaaggagatt ttccatgaca accgcaggtt ggttggtgat ggtggtatcg
     4981 ataaccacag tcactgtatt ttttgggtat aacctttatc gttcgatgcg cgacggcaaa
     5041 tagttaatga agtttttctt ctacgcgttt gttattttta gctctgcgct ttttgcatct
     5101 cctcaaaaaa gacgactcat catttggtcg atcgacggtt ttgcggcagc ttatctcaca
     5161 aatcccgagt ttcaaaaaat caaagtctgg caacgcctcc tcaaacgcgc ccaacttttt
     5221 acgccggttg agacagttat ccccgcgttg acgtaccctg cgcacaccgc gatggtcacc
     5281 ggccacgaaa cagcaaacca ccgcctttac gg
//
LOCUS       DAOMPD010000154         5292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6561_length_5292_cov_2.758259, whole genome shotgun sequence.
ACCESSION   DAOMPD010000154 DAOMPD010000000
VERSION     DAOMPD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6561_length_5292_cov_2.758259"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLY93_06615"
     CDS             complement(<1..1229)
                     /locus_tag="PLY93_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRP69186.1"
                     /translation="MAEKMKITPKKIVLKSNASKDSIAGKISAQKVREDQLREEARKQ
                     QSRSQPTPATSTAAPVQPSQKPAVVKRSVEMRQPPRPRTVFVQDERQPKTEAEEREIK
                     KGPAPKPGQDAKKKSNLREDILKKEEHKKFFIGQKQKDKRKHEPVQSVSSVPSKIKIT
                     EFVQVGELAKKLNVRAPEIIAKLMAMGVMATITQSIDAETATLLAAEYDCQIEVISLY
                     EETLIQEEEDNAEDLVNRPPVVTIMGHVDHGKTKLLDALRHADVAASEAGGITQHIGA
                     YQVTRPKGKITFLDTPGHAAFSAMRARGAQVTDIVVLVVAADDGVMPQTEEAISHAKD
                     AEVPIIVAINKIDKPTANVDRIKQDLSKFNLLPEDWGGTTPFIPISALQKLNLDKLED
                     AILAQAEIMETSANPKKR"
     gene            complement(1262..2701)
                     /gene="nusA"
                     /locus_tag="PLY93_06620"
     CDS             complement(1262..2701)
                     /gene="nusA"
                     /locus_tag="PLY93_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRP69187.1"
                     /translation="MARAKKATAEKKRSFFEDIQQVTQDKGFDKQEILEIVEAALIAA
                     YKRKYRTIDNVRIVMDKDKDDVYAIARRVVVENIVLPGMQILFDDAKKIQPDIKLGDE
                     LDVVEYPLEFGRIAAQAAMQVVAQRLRQLDQDKIRAAYESKIGEIMNGFILRKRGDTV
                     YVDLGKVEAIMPVKHQIPKERYRVEDKIKVLLHSIEQDKGGGPLRVIVSRADRQFVQK
                     LFEKEVPEIFEKIVTIKTIGRIPGERSKVVVASERQNVDPVGACVGVRGVRIQAIVRE
                     LGTERIDIVEWSSDPRELITNALTPATPSLVKADTTSREALAVVPDKDLAIAIGREGT
                     NVKIASYITGYKIDVKSESQFSQEMSSPEARKRLDEIFEVVVPEEQPVEDEGTPLTAL
                     PGLAARIIRILKENNIKYIEDLVSIDEEQLVNIEGIGRATAKRIMEIISENVEFEDDE
                     DEEEASEEQESKTNNTTEERQELDSTPKG"
     gene            complement(2730..3269)
                     /locus_tag="PLY93_06625"
     CDS             complement(2730..3269)
                     /locus_tag="PLY93_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69188.1"
                     /translation="MNTVDIAKITEDQITKCAKAALLEALTLHKITFFHSKGNMRIQV
                     ELDKPSDKYGSVNIRECETYARALKESLDAYELKSGLNLNYSLEVSSAGAERELLSIA
                     DVERFCALPLALVFTDEKDKVRSEVVHAIAFENDSITFKLADCKWNRKQSTVKKILSA
                     EPYSVPWNRIKKIRLHLDV"
     gene            3568..3720
                     /locus_tag="PLY93_06630"
     CDS             3568..3720
                     /locus_tag="PLY93_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69189.1"
                     /translation="MRNRGITLQSYKEDIQRGYRSLELGEGHALKHAMILKMKSDEAK
                     KSTHRS"
     gene            3847..>4550
                     /locus_tag="PLY93_06635"
     CDS             3847..>4550
                     /locus_tag="PLY93_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP69190.1"
                     /translation="MLESLTVKDFALIRDLNIQFGQGLNLITGETGAGKSVILGALSL
                     VLGYKATTDMIRSGSKRAIIEASFAMPPDSRTGAKALRQLLADQGLEDDDGNLIVRRE
                     ISIEGKGRSFVNARQVPSSVLREIGKYLVDIHGQNEHQNILSVEQHRAVLDRYAHLGD
                     LVQEMRKLHKAREEARQKLSSVTLSEDDKARRLDILQHEIRELESAKIDDVREIDELI
                     QRERSLANAETLVTDL"
     assembly_gap    4551..4560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4561..>5292
                     /locus_tag="PLY93_06640"
     CDS             <4561..>5292
                     /locus_tag="PLY93_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69191.1"
                     /translation="IHDILGGEAGFISRGGTIERLLETDSQYDTSLADVLSQFRESYY
                     ALEEVTRTVRNKAESYSLDPEELEALRERIGLLQSLARKYGGNVTPTAAFTKPTESNY
                     ADALERTKAYFQKAKNELTGIELSGAEEERLKQEIKNLNAQMKDKALYLSEKRRLASL
                     ELEQKVEAELKALGMEETQIKISIRWEYGEGGTVTDTARAKTEGDETERKYIITQTGL
                     DIVEFLMASSARDTLRPLRNIASGG"
BASE COUNT         1249 a         1304 c         1279 g         1450 t
ORIGIN      
        1 cgttttttag gattggcgct ggtctccatg atttccgctt gggctaaaat ggcgtcttcg
       61 agtttatcta aattcaattt ctgtaacgca gaaatgggga taaaaggggt ggtcccaccc
      121 cagtcctcgg gaagcaaatt aaatttggac aaatcttgct tgatgcgatc gacgttggcg
      181 gtgggtttat cgattttgtt gatcgccaca atgatgggca cctcggcgtc tttcgcgtgc
      241 gagatggctt cctcggtttg tggcatcacg ccgtcgtcgg cagcaacgac aagcaccaca
      301 atgtcggtga cttgcgcacc gcgcgcacgc attgcggaga aggcggcgtg gccaggagtg
      361 tccaagaaag taatttttcc tttggggcgc gtcacttggt atgcaccaat gtgctgagta
      421 atgccacccg cttcgctcgc ggcaacgtcg gcgtggcgca gcgcgtcgag gagtttcgtt
      481 ttaccgtggt cgacgtggcc catgatggtg accaccggcg ggcggttcac caagtcttcg
      541 gcgttatctt cttcttcttg gatgagagtt tcttcgtata gtgaaatcac ttcgatttgg
      601 caatcgtatt ccgccgccaa aagcgttgca gtttctgcgt cgatggattg cgtgattgtc
      661 gccataacgc ccatcgccat gagtttagcg ataatttccg gtgcgcgcac attgagtttt
      721 tttgccagtt cgcccacttg cacaaactcg gtaattttta ttttagacgg caccgatgaa
      781 acgctttgca ccggttcatg cttgcgtttg tctttctgtt tttgtccgat aaagaatttt
      841 ttatgttctt ctttttttaa tatatcttcg cggaggtttg atttcttttt ggcgtcttgc
      901 ccaggtttcg gcgcagggcc ttttttaatt tcacgttctt ccgcttcggt tttcggttgc
      961 cgttcgtcct gcacaaatac cgtgcgcggg cgcggtggtt gccgcatttc aaccgatctt
     1021 tttacaaccg ccggtttttg tgacggctgc actggcgccg ctgtactggt tgccggtgtg
     1081 ggttgtgaac gactctgttg ttttcgtgct tcttcgcgca gttgatcttc acgaactttc
     1141 tgcgcgctaa ttttacccgc aatcgaatct ttcgaggcgt tgctcttgag cacgatcttc
     1201 ttgggcgtta ttttcatttt ttctgccatg cgcgctattt gttccttaag tgtgacttga
     1261 cttagccctt aggcgtcgag tcaagttctt gcctttcctc ggttgtattg tttgttttgc
     1321 tttcttgttc ttcactcgct tcttcttcgt cttcgtcgtc ttcaaattcg acgttctcgg
     1381 agataatttc catgatacgc ttcgccgttg cacggccaat gccttcgatg ttgacaagtt
     1441 gttcctcgtc gatggaaact aaatcttcga tgtattttat gttattctct ttcaaaatgc
     1501 gaatgatacg cgcggctaaa ccgggcaacg ccgtcaacgg tgtgccttcg tcctcaacgg
     1561 gttgttcctc gggcaccacc acttcaaaaa tttcatccaa tcgttttctc gcttcgggcg
     1621 acgacatctc ttgtgagaat tgcgattcag actttacatc gattttatag ccggtgatat
     1681 acgatgcaat tttcacgttt gtaccttcgc ggccaatcgc aatcgctaaa tctttgtctg
     1741 gcacgaccgc gagcgcttca cggctcgtgg tgtcagcttt cacaaggctg ggggttgccg
     1801 gcgtgagcgc gttagtgatg agctcgcgtg ggtcagatga ccactcgacg atgtcgatgc
     1861 gctctgtgcc caactcgcgc acaatggctt ggatgcgcac gccgcgcaca ccgacgcaag
     1921 cacccacagg gtcgacgttt tgcctttcag aggcaacgac tactttactg cgctcgccag
     1981 ggatgcggcc aatggtttta attgtaacga tcttctcaaa gatttcagga acttctttct
     2041 cgaaaagctt ttgcacgaac tgacggtcgg cgcgagagac aatcactcgc aaagggccgc
     2101 cgcctttgtc ttgctcgata gaatgtaaga gtacttttat tttgtcttca acgcggtaac
     2161 gctcttttgg aatttgatgc ttgaccggca tgatcgcttc cactttgccc aagtcgacgt
     2221 acactgtgtc tccgcgctta cgtaaaataa aaccgttcat aatctcaccg attttacttt
     2281 cgtacgccgc gcgaatttta tcttggtcta actggcgcaa gcgttgtgca actacttgca
     2341 tcgcagcttg tgcggcaata cgcccaaact ccagcgggta ctcaaccacg tcgagctcgt
     2401 cgcccaattt tatgtcgggc tgtatttttt tcgcatcgtc gaataaaatt tgcatgcctg
     2461 gtaaaacgat gttctcgacg acgacacggc gtgcgatagc gtaaacatcg tccttgtctt
     2521 tatccatgac gatacgcacg ttatcgatgg tgcggtattt acgtttgtac gccgcaatga
     2581 gcgccgcttc gacgatttct aaaatttctt gtttgtcaaa acccttgtct tgggtgactt
     2641 gttgaatgtc ttcaaagaag gaccttttct tttctgcggt cgcttttttt gctcgtgcca
     2701 tatatctcct ttaatattcc caaactatcc tatacgtcta aatgtagtcg aattttttta
     2761 atacgattcc atggtaccga atacggctct gcgctcaaga tttttttcac ggtagattgc
     2821 tttcggttcc atttgcaatc cgccaacttg aaagtaatac tgtcgttttc aaaagcaatc
     2881 gcatggacaa cctcggagcg caccttgtct ttttcgtcgg taaataccaa cgctaacggc
     2941 aacgcgcaaa accgctctac atcggcgatt gagagcagtt cgcgctctgc ccccgccgac
     3001 gaaacctcga gtgaataatt gagattcaat ccacttttta gttcgtaggc gtctaaactt
     3061 tcttttaacg cgcgcgcgta ggtttcgcat tcgcggatat tcaccgagcc atatttatcg
     3121 ctcggtttat cgagttcaac ctgaatacgc atgttaccct tgctgtgaaa aaaggtaatt
     3181 ttatgcaacg tcaacgcttc aaggagcgca gccttagcgc acttagtaat ttggtcttct
     3241 gtaatttttg caatgtctac ggtattcatg ctgcgctaat agttttgaaa agaggtttta
     3301 ccacttttct ctatttcaaa aaaaaagcgg caaacgccac ttctccacga accagacccc
     3361 cgcaacgaaa cagggtgtca aacgattcaa aacttgcaaa ggtgaagctc aggccaactg
     3421 tcggtagaga tatcaaagtc gatggtacaa aaattaaagc gagcgcggcg aacgatcaaa
     3481 catatacacg tcaagaatta gagatgcgca aagctgtgtt agagaaaaaa atcttggatt
     3541 atcttaacgg acaccgatgc gcgtttcatg agaaatcgcg gcataacttt acaaagttat
     3601 aaagaagaca tccaacgcgg ttatcgttct cttgagttgg gtgaagggca cgcgctcaag
     3661 catgcgatga ttctaaaaat gaaatcggac gaagcgaaaa aaagcactca tagaagttaa
     3721 actcaccgcc acgccaacga attttggcaa attagtccgc tccgcattga cagcgaaagt
     3781 aaaaagttta cggttgccgt cgagtctcaa aggtgaaatc ttactgcttt aaggctgttt
     3841 tcacgtatgc ttgaatcgct caccgttaaa gactttgctc tcatccgtga tttgaatatt
     3901 cagttcggtc agggtttaaa tctcatcacc ggcgaaacgg gtgcgggtaa atcggttatt
     3961 ttaggcgcac tctcattggt gttgggctac aaggccacca ccgacatgat acgttcgggc
     4021 tcaaaacgcg ccatcatcga ggcatcgttt gcgatgccac ccgattcgcg tacgggggca
     4081 aaggcgttgc gtcaactttt ggcagatcaa gggctcgaag acgacgacgg caatctcatc
     4141 gtacgccgtg aaattagcat cgaaggcaaa ggccgcagct ttgtgaatgc acggcaagta
     4201 ccgtcgtcgg tgttgcgtga aatcggtaaa tatctcgttg atatacacgg tcaaaacgag
     4261 caccaaaata ttttaagtgt cgaacaacac cgcgcagtgc tcgaccgtta tgcacacttg
     4321 ggcgacttag tgcaagagat gcgtaaactc cataaagcgc gcgaagaagc gcggcagaaa
     4381 ttatcgagcg ttacactttc agaggacgac aaagcacgtc ggctggatat tttacagcat
     4441 gaaatccgcg agttagaaag cgctaaaatc gacgacgtgc gcgagataga cgaactcatc
     4501 cagcgcgaaa gaagtttagc gaatgcggag acactcgtca ccgatttaaa nnnnnnnnnn
     4561 ccatccacga tattttaggc ggcgaagcgg gttttatttc gcgcggtggg accatcgagc
     4621 gccttttaga aaccgattcg caatacgaca cttcgctcgc cgatgtgctg agccaatttc
     4681 gtgagagtta ctatgcgctc gaagaagtga cacgcacggt gcgcaacaaa gccgaatcgt
     4741 actcgctcga ccccgaagaa ctcgaagcgc tgcgcgaacg tatcggcctc ttgcaatcgc
     4801 tcgcgcgcaa gtacggtggc aatgtgactc ctactgccgc gtttacaaaa cccaccgaaa
     4861 gcaactacgc cgacgcgtta gagcgcacca aagcgtattt tcaaaaagca aaaaacgaac
     4921 tcactggtat cgagctttcg ggcgccgaag aagaacgtct caagcaagaa atcaaaaatt
     4981 tgaatgcgca aatgaaagac aaggcgttgt atctttctga gaaacgccgt cttgcgtcgc
     5041 tcgagctcga acaaaaagtc gaggcggagc tgaaagcgct cgggatggaa gaaacgcaaa
     5101 ttaaaatttc catccgttgg gaatacggcg aaggcggcac tgtcaccgac accgcgcgcg
     5161 caaaaacaga aggcgacgaa actgagcgta agtatatcat tacacagacg gggctcgaca
     5221 ttgttgaatt tctcatggca tcgtcggcgc gcgacacctt gcggcctttg cgcaacattg
     5281 catccggtgg cg
//
LOCUS       DAOMPD010000155         5292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6562_length_5292_cov_2.597479, whole genome shotgun sequence.
ACCESSION   DAOMPD010000155 DAOMPD010000000
VERSION     DAOMPD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_5292_cov_2.597479"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1620)
                     /locus_tag="PLY93_06645"
     CDS             complement(259..1620)
                     /locus_tag="PLY93_06645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRP69192.1"
                     /translation="MARKIKFDRDGDAIVIVGAVRTPVGQAGSALSHKHSFELGSLVV
                     DEVLKRTKLDKNKIDGVVAGEIGQSSKAPNAARVISVKNELPLNASAVTIANNCVSGF
                     EAVFEAARRIITGENDIMMVIGEESMSNFPIYLDNVKRNKKTATVEKLKANWAEVPNM
                     TDIELIDGVAEGLNDPIRDAMMFATAEIVAQKLDLKREDLDNYAHGSYKKAHDALVAG
                     KYDKYLLPVKHGENDSETLDKDEFVMSKTGFIEKPERFAKSPVIYEKMPGGLKSLYDK
                     FGKWIGKKYDESKKPVVTLFNACPQSDGAGAVIMTTESKAKELGLPILAKVKGWGYAG
                     VDPAVMGLGIAYSMKQALENTGISWDDINKYEIHEAFAATALGSMVVARDEFKYDLVS
                     RLEKGDVNPNGGTLAIGHPLGATGIRVVINQLLDLEAGATRTMGAICAGGGVGGALIL
                     EKA"
     gene            complement(1695..2610)
                     /locus_tag="PLY93_06650"
                     /pseudo
     CDS             complement(1695..2610)
                     /locus_tag="PLY93_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(2621..3376)
                     /locus_tag="PLY93_06655"
     CDS             complement(2621..3376)
                     /locus_tag="PLY93_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRP69193.1"
                     /translation="MNTLVLIKQVPDTEAKINVAGGKISEAGIKWGISPYDEIALEEA
                     IRMKEKSGGTVTAIAVGDDSVQNALRSAFAMGADTAIHVKVDAYQMLDSFGIAKAIQG
                     AIEGADYKVILAGRQGSDSDNGQVPLILATLNGWGCIPFAKKVEVAGDKATVECEADG
                     GEAVYESSLPVVITANAGLNEPRYPSLKGIMASKKKPIESKTASIAGETKIEVVGFEP
                     PPARPAGRTIDGADAAEKAKALVKALREEIKVI"
     gene            complement(3427..3795)
                     /locus_tag="PLY93_06660"
     CDS             complement(3427..3795)
                     /locus_tag="PLY93_06660"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRP69194.1"
                     /translation="MQNKNTLSGITAGRKSVSAENIYLFKTEVLRALSDETRQGIIIL
                     LGKHGSLCVNDLTAHFNVGRPTVSHHLQVLKEAKVVKSEKQGKEMHYSLNLRNIKRSL
                     RGLDKVLDSIDGETALTTRR"
     gene            complement(3927..4778)
                     /gene="tatC"
                     /locus_tag="PLY93_06665"
     CDS             complement(3927..4778)
                     /gene="tatC"
                     /locus_tag="PLY93_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRP69195.1"
                     /translation="MPLDQDIEEEKARQSAANAELNKLRRDQGFMPMSAHLEELRKRL
                     FRSILWILGGAVVSYFFYTKVWDFLMGPVAQLILKSRLPDMPPVKIMTTRLGDNFAIE
                     FKILLLAGFILAFPFIIFEVWRFITPALSASFRRKGSIVLLASVILFWCGVIFARLVT
                     WGTVADFLIFQWLPPPVIISATEVIRPEVNLTVSDYLSFFTGFHLAFGLSFQLPVVSV
                     LLGSLGIVSSGMYFRYWRHAIVVLCVFSIVIMPPDIFAMLTLMAPMLLLYFLSGFLVK
                     FIERKQE"
     gene            complement(4780..>5292)
                     /locus_tag="PLY93_06670"
     CDS             complement(4780..>5292)
                     /locus_tag="PLY93_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRP69196.1"
                     /translation="AKSGADALIAVAQGAGGHAGAISPFALIPYIKEEIPELPLVASG
                     AISSGKQMAAAFALGADAVYVGTRLIATPEAHAYDEYKQMLIDSAPEEIIYSPKISGI
                     PANWLKRSLAKLDPNFKPDVGLSVAAKSWKEVWSAGHGVAQIKSIKSASLIIEKMATE
                     YFTVKKQLPE"
BASE COUNT         1315 a         1420 c         1186 g         1371 t
ORIGIN      
        1 gaggtcacgc tcttcggcgg aagggcggat gtctcgttta gcgaaatcat aaacctgttc
       61 gcggaattcc attacttcgt cgggcaggcg gtatttcatg ctcgtcaaaa atagaatccg
      121 acagagttag acaatcaaaa tgatacttag tgaggagcct gtggatgagc gtcactcccc
      181 tctccctacc tacaccgaac ggagtcgaag tatagagagg gtcgagggtc gagaggtggg
      241 atttctaccg caatgcgctc aagctttttc cagaatcaaa gcgccgccca caccgccacc
      301 agcgcagata gcgcccatcg tgcgggttgc tcccgcttcg agatctaaaa gttggttgat
      361 caccacgcgg ataccggttg ctcccagtgg atggccaatt gccagtgtgc caccgttcgg
      421 gttgacatcg cccttctcca aacgagacac gaggtcgtat ttgaactcgt cgcgtgccac
      481 aaccatggaa cctaatgcgg tcgccgcaaa tgcttcatgg atttcatact tattgatgtc
      541 atcccagcta atgccggtgt tttctaacgc ttgcttcatg gagtatgcaa tacctaaacc
      601 cataaccgcg ggatcgacac ctgcataacc ccagcctttc actttcgcta aaatggggag
      661 ccccaattct ttcgcttttg actcggtagt catgatgact gcaccggcgc cgtcggattg
      721 tggacatgcg ttgaaaagag tcacaacagg tttttttgac tcgtcgtatt ttttaccaat
      781 ccactttcca aatttgtcgt aaaggctttt taagccgccc ggcatttttt catagatgac
      841 cggtgacttg gcaaagcgct cgggcttttc gataaaacca gttttactca tcacaaactc
      901 gtctttgtcg agagtttcag aatcgttttc gccgtgtttt accggcaaca ggtatttatc
      961 gtactttccc gcaacgagtg catcgtgcgc ctttttatac gagccgtgcg catagttatc
     1021 caaatcttcg cgcttgaggt cgagcttttg agccacaatc tccgcagtcg caaacatcat
     1081 cgcgtcgcgt atcgggtcgt ttaacccttc tgccacaccg tcgatgagtt cgatgtcagt
     1141 catgttgggc acctctgccc agttcgcctt aagtttctca acggtcgctg tttttttatt
     1201 acgtttaacg ttgtcgaggt agataggaaa gtttgacatc gactcctcgc caatcaccat
     1261 catgatgtcg ttttcgccgg tgataatacg gcgcgctgct tcaaagactg cctcaaaacc
     1321 ggacacgcaa ttattcgcaa tcgtcaccgc gctcgcattg agaggaagtt catttttaac
     1381 agaaatcaca cgcgcggcgt tgggagcttt ggacgactgt ccaatttcgc cagcaacgac
     1441 accgtcgatt ttatttttat cgagtttagt acgcttcaat acttcgtcga ccacgagcga
     1501 acccaactcg aatgagtgct tgtgagaaag tgcagatccc gcttgtccca cgggtgtccg
     1561 gactgcgccg acgataacaa tcgcatcgcc gtcacgatca aatttaattt ttcttgccat
     1621 aatttactcc ttccgcgctt cgcgcggata aaaaataact taaagggatg cgcagcatcc
     1681 ccttaacaat cccactattt acccagcgca gctttaagct cttcttttaa gggcggcagc
     1741 acgttgaata aatcgtccac cacaccgtat gttgatcgcc acaatgaact tcgatgaacc
     1801 cataccagcc atgtgttgaa ttgcgcctga aatacccaca gccacgtaaa ggttggggga
     1861 caccgtttga ccggtttgac caatctggag agtgtggtcg caccaaccgg catcgaccac
     1921 cgcacgggtt gcacccacgc ctgcgcccaa gatgtcggcg agctgttcga taagagggaa
     1981 attttcagga cctttgattc cacggccagc cgaaaccacg atgtccgctt cggtgagtga
     2041 aacgcgtgtg ccaccggttg cttcaaactt cacttgtttt gcttgagaag cgcctccatc
     2101 cacggtaatt ttttccactt tacctgcgcc agctgcatcg gcaatcgggt gcgcgttggg
     2161 gcggatggtc accacttgcg gtgaagattt tactttaaga gtgacaaacg cttttcctga
     2221 atagacgggt ttgcggatct tcacccgatc gccgtccata gaaatttcta ccgcgtctga
     2281 aacaacgccc gtatttaaga gcgtcgcaat gcgcgatgaa atgtcgcggc caatccacga
     2341 gtgaggaagg atgacaattt caaaaccatt gttttttaca acgtcgacaa tcgcttttga
     2401 atatgcttcg ccattgtacg ttgcgatttc acccacgtac accgtgtccg cgccgtgctt
     2461 tgcaacctcg gctgcgcctg cttctgcgcc cgaaccgatg aggattgcag ccacttcacc
     2521 gcctaatttt tttgcgacgc ttgttgtttc gcgtgaggct ttctttaaca ccccacctga
     2581 gatttcacca actgctaata tttttgccat ggggtactcc ttaaatgact ttaatttctt
     2641 cacgcagcgc ttttacaagc gctttcgctt tttctgcggc atctgcacca tcgatagtgc
     2701 gacctgcagg ccgtgccggt ggtggctcaa aaccaaccac ttcgatttta gtttcaccag
     2761 cgatgctcgc tgttttgctt tcaatcggtt tctttttaga agccatgatg cctttcagag
     2821 aaggatagcg cggctcgttg agaccggcgt tggcagtgat gacaacggga agtgaagatt
     2881 catacaccgc ttcgccaccg tctgcttcgc actccactgt ggctttgtcg cctgcgactt
     2941 cgactttttt tgcaaaagga atacatcccc aaccgttgag cgtcgctaaa atcagaggca
     3001 cttgaccgtt gtctgagtct gaaccttggc ggcctgcaag aataacttta tagtcggcgc
     3061 cttcgattgc gccttggatt gctttcgcga taccaaaact gtcgagcatt tggtaggcgt
     3121 cgacttttac atggattgcc gtgtcggcac ccatagcgaa tgcagagcgg agcgcgttct
     3181 gtacggaatc gtcaccgacg gcaattgcag tcaccgtgcc gcccgatttt tctttcatac
     3241 gaatcgcttc ttccagagca atctcatcgt agggtgagat accccattta atgcccgcct
     3301 ctgatatttt gccgccagcg acgttaatct tcgcctcggt gtcgggcact tgtttaatca
     3361 aaaccagtgt gttcatccgt ttctccttgg gtcgcttact gcgtcgcgac tgtttgcttt
     3421 tttactctag cgtcgtgtgg tgagcgcagt ctcaccgtca atgctatcaa gaactttgtc
     3481 taagcctcgt agcgaacgct ttatattacg caagttcaaa gaataatgca tctctttacc
     3541 ctgcttttct gacttgacca cttttgcttc ctttagcact tgtagatggt gtgatacggt
     3601 gggacgcccc acgttaaagt gagcggtcaa atcgtttaca cacaaactac cgtgcttccc
     3661 taaaaggatg ataatcccct gacgggtttc atccgaaagc gccctcaaaa cctctgtttt
     3721 gaaaagataa atgttttccg ctgaaacaga tttacgccct gcggtaatac ctgaaagcgt
     3781 atttttattc tgcattgcat ttatcggggc tcagttgcag ccaaacgcgt cgaaattcat
     3841 cgacgcgttg ccaatttggg aaattcacac agagggcaaa ggaaaaagag tgttatgagc
     3901 ctgtgcgcca aaaattaaaa actttcttac tcttgcttcc gttcgataaa cttcactaaa
     3961 aaaccagata gaaaataaag cagtaacatt ggcgccatga gcgtcaacat tgcaaaaata
     4021 tcaggcggca taatcacaat cgagaaaaca cataacacca caatcgcatg ccgccaatac
     4081 cgaaaataca ttccactgga aacaatcccc aacgaaccca atagcactga aactaccggt
     4141 aactggaaac tcaatccaaa cgccaaatga aaccctgtaa aaaacgagag ataatccgaa
     4201 acagtgagat taacttcggg acggatgacc tccgtggctg aaataataac cggcggcggg
     4261 agccactgga atatgagaaa gtcagccacc gtgccccacg tcaccagacg tgcaaatatg
     4321 acgccacacc agaataaaat tacagacgcc aaaagtacaa tacttccctt gcgccggaat
     4381 gacgccgata gcgcaggggt gataaaccgc catacctcaa aaataataaa cggaaaagca
     4441 aggataaatc ccgctaaaag gagtatctta aactcgatcg cgaaattatc ccccaaacgt
     4501 gtggtcataa ttttaacggg tggcatgtcg ggtaagcgcg acttgaggat gagttgagcg
     4561 accggcccca taaggaaatc ccacactttt gtataaaaaa aataactcac taccgcaccg
     4621 cctaaaatcc acaggatcga acggaaaagc cgcttgcgca gctcctccaa atgtgcgctc
     4681 atcggcataa agccttgatc acggcgcaac ttattgagtt cggcgttggc ggcgctttgt
     4741 cgtgcctttt cttcttctat atcttgatca agtggcattt tattctggga gctgcttttt
     4801 tacagtaaag tactcagtcg ccattttttc aataattaag gaagcgctct taatactctt
     4861 aatttgagcc acaccgtgcc ccgccgacca cacctctttc caagactttg cggccaccga
     4921 caatccgaca tccggtttaa aattgggatc gagcttagcc agagagcgtt ttaaccaatt
     4981 tgccggaatc cccgaaattt taggagagta aataatttcc tcaggggccg aatcgataag
     5041 catctgctta tactcgtcgt acgcgtgtgc ctcaggggtg gcaatcagcc gcgtccctac
     5101 atacaccgca tctgccccca atgcgaacgc tgccgccatc tgctttcccg acgaaattgc
     5161 ccccgatgca acaagtggca attcagggat ttcctcttta atataaggaa tcaatgcaaa
     5221 aggactaatc gcccccgcgt gccctcctgc cccctgcgcc acagcaatca acgcatcggc
     5281 acccgatttt gc
//
LOCUS       DAOMPD010000156         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6586_length_5280_cov_3.073493, whole genome shotgun sequence.
ACCESSION   DAOMPD010000156 DAOMPD010000000
VERSION     DAOMPD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_5280_cov_3.073493"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLY93_06675"
     CDS             complement(<1..493)
                     /locus_tag="PLY93_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69197.1"
                     /translation="MTLFRNKYRVESARLKGYDYSSPGFYFVTICTKNREHFFGEIKN
                     NVMNFSNMGQIATKFWREIVDHFPHVKLDEFIIMPNHLHGIIQIYHDGRDAINRVSTD
                     LKIENPSLSKIIRWYKGRSTFEIRKSICHDFAWQSRFHDHIVRNEQSLNQIRLYIQQN
                     PFHW"
     gene            complement(490..3606)
                     /locus_tag="PLY93_06680"
     CDS             complement(490..3606)
                     /locus_tag="PLY93_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRP69198.1"
                     /translation="MSLPARIDDLVQHFAQHIDSFKSGNYNETRARIEFIDPFFKELG
                     WDIENTQGFAETYKDVIHEDAIKVEGSTKAPDYSFRIGGQRKFFLEAKKPAVNVKDDI
                     APAYQLRRYAWNAGLPVSILTDFEEFAIYDTTIRPNIKDKASTARIFYCTYKEYAEKW
                     DYIASIFSKDAILKGSFDQFLKDAKKKKGTTAVDKEFLKEMEKWRTDLAKNIALRNTI
                     DIHALNYVVQATIDRILFLRICEDRNIEEYGRLQKLTSAANIYKKLTDIFEQADEKYN
                     SGLFHFNKEKENPSSPDEISLRLKIDDKILKEIITALYYPSPYEFSVISADILGNVYE
                     QFLGKVIRLTSGGQAKVEEKPEVKKAGGVYYTPQYIVKYIVENTVGELLKGKTPMMVA
                     GKVKGHNPLRILDPACGSGSFLIYAYQYLLDWHRDWYEKDIKSKGESQAKKWSDEVYQ
                     GPGNHWYLTTREKKRILLNNIFGVDIDHQAVEVTKLNLLLKVLEGENRETLGTNLKLF
                     QERALPDLSGNIKCGNALIGSDFYNLRRDAINRVSTKIDSQQPLFEISDTERMKVNAF
                     DWQVEFPEVFASTGSANAGGFDVVIGNPPYVRQETLGADFKNYAARAYETYAGTADLY
                     TSFIEKSLSLLSSTGVYSIIVANKWLRANYGEPLRRFLKKKKIMRIIDFGDLPVFTGA
                     TTYPCIIQIANGSSAGQTSQPADFKTSLIKTLEFENLAHEVQKTQFTVSIQNLDDKGW
                     SLVNESEAALLTKIKNAGVPLGEYVEGKIYRGVLTGLNEAFVIDDETKNRLIKEDKNS
                     AEIIKPFLAGRDIKRYALPNCNKYLIFTRRGIDIKKYPAIEKHLLQFKTQLMPKPANF
                     KGSDWPGRKPGSYKWYEIQDSVDYYTEFEKESIMYPDISQVGNFTIRTAGTYLTNTGY
                     MISSSDRYLLGLLASKLSLFYYSRQANANRGNFFRWFTDVLELNPIRRINETNPSEIQ
                     VRDRIIALATQMLSLKENLSVGQVLAPAQREQLQRQIGTTDREIDRLVYELYGLTDEE
                     IAIVEGEK"
     gene            complement(3884..4378)
                     /locus_tag="PLY93_06685"
     CDS             complement(3884..4378)
                     /locus_tag="PLY93_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69199.1"
                     /translation="MNKSSIASILMSLGEIFINIKDHSGQKIGSIFAQHYPKMNRIQI
                     AISDFGVGIPETVKRRFVAANDNEAILKAVEYGFSTQSTPQNRGVGLTTLISNVVKNN
                     MGSMAIHSGRGAIQFELTNKEISAKSILTKANFQQFYPGTLFSVILRTDTIEKLADEE
                     EFAW"
     gene            complement(4824..>5280)
                     /locus_tag="PLY93_06690"
     CDS             complement(4824..>5280)
                     /locus_tag="PLY93_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRP69200.1"
                     /translation="KGFLGDAHTALAAVAVAMVLKSCGFGMVLFLTALKNISPNIFEA
                     ADLDGMTWWQKFRYIRVPLVKPVILFLVVTGILGCLNAFTEFYIMTEGNPVTLFAGET
                     VGATRVSGYLLFNRFTEMNYGSAAAMSYFLLIFALVVSFINYRLLRGKA"
BASE COUNT         1478 a         1180 c         1042 g         1580 t
ORIGIN      
        1 gccaatggaa tgggttttgt tgaatataca accgaatttg gtttagcgat tgttcattgc
       61 gaacgatgtg gtcgtggaat cgggattgcc atgcgaaatc gtggcagatc gattttcgaa
      121 tttcaaatgt tgaccgtccc ttataccaac ggattatttt ggataacgat ggattttcta
      181 tttttaaatc cgtagagacg cgattaatcg cgtctctacc atcatgataa atttgaatta
      241 ttccatgcaa atgattcggc atgataataa attcatctaa tttcacatgg gggaaatgat
      301 cgacgatttc acgccaaaat tttgtcgcaa tctggcccat attagaaaaa ttcatcacat
      361 tgtttttgat ttcaccaaag aaatgttcac gatttttggt gcaaatcgtc acaaaataaa
      421 accccggcga tgaataatcg taccccttta gccgtgcgga ttcgacgcgg tatttattgc
      481 gaaaaagagt catttctctc cctccacaat cgcaatctct tcatcagtca acccgtacaa
      541 ctcatacacc agtcgatcaa tctcgcggtc ggtagtgccg atttggcgct ggagttgttc
      601 gcgttgggca ggcgcaagca cctgcccaac gctcaggttt tctttgagcg atagcatttg
      661 agtggctaat gcaataatac ggtcgcgcac ttgaatttcg ctgggattgg tttcgttgat
      721 gcggcggatg ggatttaatt caagtacatc tgtaaaccaa cgaaagaaat taccccgatt
      781 cgcgtttgct tgtcgcgaat aataaaaaag agataacttg cttgcaagaa ggccaagtaa
      841 atatctgtca cttgagctaa tcatgtaacc tgtatttgtt aggtatgtac ctgctgtacg
      901 tattgtgaaa ttaccaactt gtgaaatatc agggtacata atactttctt tctcaaattc
      961 ggtatagtaa tctacactat cttgtatttc ataccactta tacgaacccg gcttgcgacc
     1021 tggccagtca gaacctttga aatttgctgg cttcggcatg agttgtgtct tgaattgtaa
     1081 gaggtgcttt tcaatcgcag gatatttttt tatgtcgata ccacgccgtg taaaaataag
     1141 atatttgtta caatttggta aagcataccg tttaatatcg cgacccgcta aaaacggttt
     1201 aataatctct gcactgtttt tgtcttcttt aatgagccga ttctttgtct cgtcatcaat
     1261 aacgaacgct tcgttcaagc cggttaaaac tccacgataa attttaccct caacatattc
     1321 gcccaacggc acaccggcat tcttaatttt cgtgagcaag gcagcttcgc tctcatttac
     1381 caatgaccaa cctttgtcgt ctaagttttg tatcgatacg gtaaattgtg tcttttgaac
     1441 ctcatgcgca agattttcaa actccaaagt tttaatgagg ctcgttttga aatccgccgg
     1501 ttggctggtc tggccagccg aactaccgtt tgcaatttga atgatgcaag ggtatgttgt
     1561 tgcaccagtg aaaaccggca aatcgccaaa atcgataatg cgcataattt tctttttctt
     1621 caaaaaacgg cgcagcggtt cgccgtaatt agcgcgcagc catttattcg cgacgataat
     1681 tgaatacact ccggtcgacg acagcaagct caatgatttt tcaataaagg aggtgtacaa
     1741 atcggcggtg cctgcatacg tctcatacgc ccgcgccgca taatttttga aatctgcacc
     1801 caacgtctct tgccgcacat acggcggatt tccaatcacc acatcaaacc cacccgcgtt
     1861 cgctgagcct gtcgaagcga acacctctgg aaactccacc tgccaatcga acgcattgac
     1921 cttcatgcgt tcggtatctg aaatttcaaa aagcggttgt tggctatcga tcttcgtaga
     1981 gacgcgatta atcgcgtctc tacgaagatt ataaaaatca ctcccaatca acgcattccc
     2041 acacttaata ttccccgaca aatccggcaa cgcccgctcc tgaaacaact tcaaattcgt
     2101 ccccaacgtc tcgcggttct caccctccaa aaccttcagc agtagattaa gcttagttac
     2161 ctcaactgcc tgatgatcaa tatctacacc gaaaatatta ttcaacaaga tgcgtttttt
     2221 ctcgcgagtt gtgagatacc agtgattacc cggcccctga taaacttcat cgctccactt
     2281 tttcgcctga ctctcaccct tggatttgat atccttttca taccagtccc tatgccaatc
     2341 cagcaaatat tggtacgcgt agatcaagaa cgatcccgaa ccacaagcag ggtcaagaat
     2401 tctgagcgga ttatgccctt ttactttgcc tgcaaccatc ataggtgttt tgcctttcag
     2461 caattcgccc accgtgtttt cgactatgta tttgactatg tattgtggtg tgtaatagac
     2521 cccgccagct tttttaactt cgggtttctc ttcaaccttg gcttgtccgc ctgaggtgag
     2581 acgaatgact ttcccgagaa attgttcgta gacgttgccc agaatgtcgg ctgaaataac
     2641 ggaaaactca taaggggatg ggtaatagag tgctgtaatg atttctttga gtattttatc
     2701 gtcaatttta agacgaagac ttatttcgtc cggagagctt ggattttcct tttccttgtt
     2761 gaagtgaaat aagccagaat tgtatttttc atcggcctgt tcaaagatat ccgttaattt
     2821 cttatagata tttgctgcgc tggtgagttt ttgcagacgc ccgtactctt ctatattgcg
     2881 atcttcgcaa atgcgcaaaa ataaaattct atcgatcgtg gcttgcacta catagttgag
     2941 cgcgtgaata tcgatggtgt ttcttaaagc aatgttcttg gcgagatcag tacgccattt
     3001 ttccatttct ttgagaaatt ctttatctac ggcagttgtg cctttctttt ttttggcatc
     3061 tttgagaaat tggtcaaaac tgcctttgag tatagcatcc ttcgaaaaaa ttgatgcgat
     3121 atagtcccat ttttcggcgt attctttata cgtgcagtaa aatatacggg ccgttgaggc
     3181 cttgtccttt atattgggtc gaatcgttgt atcatagatg gcaaactctt cgaaatctgt
     3241 taaaatcgaa acgggcaagc ccgcgttcca ggcataacga cgaagttgat aggctggcgc
     3301 gatatcatcc ttaacattta ccgcaggctt tttggcttcc agaaagaact tgcgttggcc
     3361 cccaatacgg aatgagtagt ccggtgcctt cgttgagcct tcgaccttta tagcatcttc
     3421 gtggataacg tctttgtagg tttcagcaaa accttgagta ttttcaatat cccagcctaa
     3481 ttctttgaaa aatgggtcta taaactcgat tctggcccgc gtttcattgt aattgccgct
     3541 cttgaaagaa tcaatgtgct gcgcgaaatg ctgaactaaa tcgtcgatgc gcgcaggcag
     3601 gctcacaagt tgctcgatag tcgatttctg atggcgtcat ttatagcctt attagaatct
     3661 atgattgaaa gatgggtttt taattcactt atagaacgtt cttctgctat tttcaccaag
     3721 gaagcattga gaaacgaggt ggtaatgaag tcaaaatcct taaatgataa aactaatttt
     3781 gaatcagaac gcaatatctc tggccaagca ttcttaatct gctcgtagat taacaaaccg
     3841 tcagaattat catagcattt ggtgacgtaa tcttttagtt gaattaccat gcaaattcct
     3901 cctcatctgc taatttttct attgtatctg tcctcaatat aacactaaat aaagttcccg
     3961 gatagaattg ttgaaaatta gctttcgtca aaatactttt tgcggaaatt tccttgttcg
     4021 tcaactcaaa ttgaatagca cctcggccag aatggattgc catagaaccc atattattct
     4081 ttacaacatt tgaaatcaaa gttgttaaac caacacccct attttgaggg gtgctctgag
     4141 ttgaaaatcc atattcaaca gctttcagaa tggcttcatt gtcatttgct gcaacaaacc
     4201 gacgtttgac ggtttcaggt ataccaacgc caaaatcaga aatagcaatt tgaattctat
     4261 tcatctttgg gtaatgctga gcgaaaatac ttcctatctt ctgacccgaa tggtctttaa
     4321 tatttataaa aatctcacct aaactcataa ggatgcttgc aatggagctt ttattcatgc
     4381 gtaagcgtcg agtaagctac tcagcaaatt ttccatgcag ccaatcatgg ctgcggtcat
     4441 atgagactag ttctagcggt accgtagtca cccttactct cgaaccttgg aacaattttt
     4501 gacctaagaa aatttcaaaa aatcccgaat catctaagta ttgaattggt cgagtgttag
     4561 cgtaattaaa cttaaaactt acgcgcttcg ttgatcgaag ccattcgaaa atattactca
     4621 agactgttac accaaccggt tcaataaaat tcaaagaagt aaaatcaaaa ataatattcg
     4681 atgctttggg ttcgaggtta tcgtctaaaa cggcctttaa tagcaagtac atcgattgat
     4741 tattaaaccg attcggaatc gtaggattta tcgaattata tccattagca ggactctata
     4801 cacaccggag agaaatcacc tcctcacgcc ttccccctca aaagacggta gttaatgaaa
     4861 gataccacta acgcaaaaat taagagaaaa tacgacatcg ccgccgcaga gccgtaattc
     4921 atttccgtaa aacggttgaa aagtagatag cccgaaacgc gtgtggcgcc caccgtctcg
     4981 cccgcaaaga gcgtcacggg atttccctcg gtcatgatgt aaaattcggt gaatgcgttg
     5041 agacaaccta aaatacccgt gacgaccaaa aataaaataa cgggcttcac gagcggcacg
     5101 cggatgtagc ggaatttttg ccaccacgtc atgccatcta aatcggcggc ttcaaaaatg
     5161 ttgggcgaaa tgtttttgag cgcagttaaa aaaagtacca tgccaaagcc gcagctttta
     5221 agcaccatcg ccacagcgac cgcggcgaga gcggtatgcg cgtcgcctaa gaaacctttc
//
LOCUS       DAOMPD010000157         5263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6618_length_5263_cov_2.398233, whole genome shotgun sequence.
ACCESSION   DAOMPD010000157 DAOMPD010000000
VERSION     DAOMPD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_5263_cov_2.398233"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..882)
                     /locus_tag="PLY93_06695"
     CDS             complement(157..882)
                     /locus_tag="PLY93_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69201.1"
                     /translation="MQRRTLYSILPLLIFMSAFSLVAVPPKGKTKFSKQKEAADPATK
                     DMVLPEENTGNYGRPWKRSALDLRVGFLGGPIIPIGQASKVLETGWGGGLWASAETSF
                     IKLPKSKVFENTLLIYEFSFYKVKGTSVSSKIGQDTDADFVTNAGAIGKAYDVQFSPK
                     FVMRFIPYLGVGLTNITSDTKTPTGNIRAVSGDIIMKYGVAAAYDFNQRWQALLTLDH
                     YIYFEKEVGMSLRINLGATMMIF"
     gene            complement(1008..>1855)
                     /locus_tag="PLY93_06700"
     CDS             complement(1008..>1855)
                     /locus_tag="PLY93_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69202.1"
                     /translation="HEELVKYLRRKKDYERLGDELIFLRAATGRQEARERVEQFEIER
                     RDLWYVKEGFSTPEREKNLVPIHIYPLRARDPLSDHPMGGIALADRTRVALEDFGRVR
                     SISREMANLPEAQMFTPENLRRLKSTYQAALNAEENPHVFHRRSLGLVLTGSFQETKH
                     GVAVESELVDADSGIRVATENFRAEGRNYLNKAAVRLAQFVFTHSPLSANILHIQEGD
                     RVLINTGSRDALPKDAVFTILDKAGNVQEFKTTRRSLDILEAKSNSSTATNHLKAGDR
                     VVLKQ"
     assembly_gap    1856..1865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1866..3012)
                     /locus_tag="PLY93_06705"
     CDS             complement(<1866..3012)
                     /locus_tag="PLY93_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69203.1"
                     /translation="MKKNMLSHRYKILQLKSYFIVGCISVLVLFFVTTSFASPSSPQY
                     YYDEAVKFMKSGDTIRASDMARESLRRNGDYVPSLLLLAQILNDSLEIKSAQQLVSRA
                     LSLDNDNEKAALLCARIELKLNNREQFENCLQIAQKKKKLNPDAESLYAQVLINNAQY
                     GIAKRKIDAILRDNPGHTETRVRLAGLYLKLKQFQKAEEEFRKIQALIPENNELAVAI
                     ARARIDAFFKDSPNGIFSAESDTALRALEALRHAYSNNNDNLSVNLMLAQLLAVTGKC
                     SDADSFLSKLIQSEKEMRSVVIFYALCNPTQGQRYLINYLRRNEDDDLTRHQEELLQL
                     SLNKKRENPIATQAARYHFQIARRAMKENADMFALASLRWTQFLFPAY"
     gene            complement(3009..>4166)
                     /locus_tag="PLY93_06710"
     CDS             complement(3009..>4166)
                     /locus_tag="PLY93_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69204.1"
                     /translation="VEFSPPELKKPRHIEWDAADNVFLIADESAGVVRMNDAGEILGA
                     LTGYKNNDGRFVEFARPYAAAFDAFRNLYVADYGASEVVQFVPQSEKFAPLYLHVEKV
                     SAAKFPVMGVYVTVSTGMGSERQSGMPNYLQNLTAEDFKVLENDASVGNLDTTYLKQF
                     DDILFASILVSRSKKMKDYETQLPWVLDHLLTKIREKDGFQIVTHGSDIRHESEFTHS
                     RLNLLQSLKRALRDDLLTDTPMRTASAALYDALTDLLLKEGKRALLYITDGAMDDDAL
                     TPYTKVRLIDYARANHIPIFVVNFENPVYADEARQTLGELAQRTGGVYFRALDIDPNI
                     ESLMRNQGEVRYLLGYQSKVKKQTRGQYIDLRVMARFRERRGLDLNGYFIP"
     assembly_gap    4167..4176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4177..5148)
                     /locus_tag="PLY93_06715"
     CDS             complement(<4177..5148)
                     /locus_tag="PLY93_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69205.1"
                     /translation="MLDTLSSFFNRHRAWSIAAFVVLSTSLAYAVFAPALGIRQNQAD
                     EEFQKGWLLFQDRKFDAAVEKFSQALRINPEFNWARRFLAEAYYFSGQKSDALDEYEA
                     LARAMPHDLTLKNHIDALTLSDDAPAEKPTEFLRVLDRTQGYRYNRPTYVGLINSDRF
                     AVLSLGAFDVGNMVSYSAQGEPFENQHRVSGKLGYPMAFAQNENEVWITDFKEDKIHR
                     MKKKPAQFFSYFFNPPALGKTGSAPLEFRAPAGLCHREGEFVVADSENNRLQRISDDG
                     KFIAQITRPADVDAMQSPFGVWCNDEAIWTTEPTLARVTHFDRYGSIV"
BASE COUNT         1351 a         1265 c         1205 g         1422 t
ORIGIN      
        1 ggcagtaatt tctttttccc tggcgtcggt gacacgcccg cacgctgcca aaagcaacgt
       61 tgttatgaaa gtaaatccta cgatgttcag tcgtttcata ctctttccct ccatctcttt
      121 agacggtaca cttaatacga ttacgacaac tttatatcaa aaaatcatca tcgtcgcacc
      181 gagattaatc ctaagcgaca taccaacttc cttttcaaaa taaatatagt gatcgagcgt
      241 taataacgcc tgccagcgtt ggttaaaatc gtacgctgcc gcaacgccgt atttcataat
      301 gatatcaccg ctaacggcac ggatattccc ggttggggtt ttggtgtccg aggtgatgtt
      361 cgtcagcccc acccctaaat aagggataaa gcgcatcacg aacttaggcg agaattgcac
      421 gtcgtacgct ttgccgatgg ctccggcatt ggtgacaaag tcagcgtcgg tgtcttgtcc
      481 aattttactc gacaccgagg ttcctttcac tttataaaaa ctgaattcat atataaggag
      541 agtattttca aaaactttag atttggggag cttgataaag ctggtttccg cagacgccca
      601 caaaccccca ccccagcctg tttcaagcac tttggatgct tggccgatgg ggattattgg
      661 cccacccaaa aaccctacgc gtaaatccag agcactccgc ttccacggac ggccatagtt
      721 gccggtattt tcttcgggca acaccatatc ttttgtggct gggtctgccg cctctttttg
      781 cttggaaaat ttggttttgc ctttgggggg taccgcaaca agagaaaagg cgctcataaa
      841 aatgaggaga ggaaggattg agtatagcgt tcttcgttgc atagtagctt cgcattcaac
      901 tgatgttgaa cgccctttat aactgaatta gacgaagaga acttacttat ttacaacaaa
      961 aaaaagtcaa aattttcacg ccgttcgttg aagggcctgt gcgccaatca ttgcttcaat
     1021 acaaccctgt cccccgcttt taaatgattg gtcgcggtgg atgaattact ttttgcttct
     1081 aaaatatcta aactgcgacg cgtcgttttg aattcctgca cattccccgc tttgtcgaga
     1141 atggtaaaca cagcatcttt gggtaacgcg tcgcgcgagc cggtattgat caaaacgcga
     1201 tccccttctt ggatatgcaa aatatttgcg cttaaaggcg aatgcgtaaa aacaaactgt
     1261 gccaaacgca ctgccgcttt gtttaagtaa ttgcggcctt cggctcgaaa gttttcagtg
     1321 gcaacgcgga tacccgaatc ggcgtcgaca agctcgcttt caacggcaac accgtgttta
     1381 gtttcctgaa agctgccggt caatacaagc cccaacgaac gacggtggaa tacgtgggga
     1441 ttttcttcgg cgttgagtgc ggcttggtat gtgcttttaa gacgccgtaa attttccggt
     1501 gtaaacattt gcgcctcggg taaatttgcc atctcccgcg aaatcgaacg cactcggcca
     1561 aaatcttcca acgccacacg agtgcgatcg gcgagtgcaa tgccacccat cgggtggtcg
     1621 ctcaaaggat cgcgggcgcg tagggggtaa atgtgaatgg gaacaagatt tttctcccgc
     1681 tccggcgtag aaaatccttc tttgacatac cacaaatcgc ggcgctctat ttcaaattgt
     1741 tcgacgcgct cgcgcgcctc ttggcgccct gtcgccgcac gtaaaaaaat gagctcgtcg
     1801 cccaaacgtt cgtaatcttt tttgcggcgt aaatacttca ccagctcttc atgcannnnn
     1861 nnnnncgtac gccggaaaaa gaaattgtgt ccaacgcaaa cttgccaatg caaacatgtc
     1921 ggcgttttct ttcatcgcac ggcgggcaat ttgaaaatga taacgcgccg cttgcgttgc
     1981 tatagggttc tcacgttttt tattgagcga aagctgcaat agttcttctt gatgacgcgt
     2041 taaatcatcg tcctcgttgc ggcgcaaata attgattaaa taccgttggc cttgtgtggg
     2101 attacaaagc gcgtagaaaa taacgacgct acgcatttct ttttcactct gaatgagttt
     2161 acttaaaaag gaatcggcat cgctacactt tccagtgacg gctaaaagtt gggcgagcat
     2221 aagatttacc gacaagttgt cgttgttgtt cgagtatgca tgccgcaatg cttccagcgc
     2281 acgcaacgct gtatcgcttt cggcggaaaa aatgccgttg gggctatctt tgaaaaaagc
     2341 gtcgatgcga gcccgcgcaa tcgccacggc gagctcgttg ttttcgggga taagagcttg
     2401 aatttttcta aactcttctt cggctttttg aaactgcttg agttttaggt aaagccctgc
     2461 taaacgaacg cgcgtttccg tatgccctgg gttatcgcgc aaaattgcgt cgattttacg
     2521 cttggcgatg ccgtattgtg cgttattgat taaaacttga gcgtatagag attcggcgtc
     2581 ggggtttaat tttttctttt tctgagcaat ttgcaaacag ttttcaaact gctcgcggtt
     2641 attgagtttc agttcgatgc gcgcgcacaa cagcgccgct ttctcgttgt cgttgtcgag
     2701 gctcagcgcg cgcgacacca actgctgcgc actttttatc tcaagcgagt cgtttaaaat
     2761 ttgggcgagt aatagcagcg acggcacgta atcgccgttg cgccgtaacg attcgcgcgc
     2821 catatcgctt gcacggatgg tgtcgccact tttcatgaac ttgactgctt catcgtaata
     2881 atactgcggt gaagacggcg atgcaaaact tgtcgttaca aaaaataaaa caagtactga
     2941 aatgcatccc actataaagt aagattttaa ctgtaaaatt ttgtatcgat gcgacaacat
     3001 attttttttc atggaataaa atacccgttt aagtcgagcc cccgcctttc gcgaaaacgc
     3061 gccataaccc gcagatcgat atattgtccg cgcgtctgct ttttgacttt gctttggtag
     3121 cccaaaagat aacgcacctc gccttggttg cgcatgagcg attcgatatt ggggtcgatg
     3181 tcgagagcac ggaaataaac accgccggtt ctttgcgcaa gctcacccaa ggtttgacgc
     3241 gcttcgtctg cgtacactgg gttttcaaaa ttgacgacaa aaataggtat gtggtttgcg
     3301 cgcgcataat cgataagacg cacttttgta taaggagtaa gcgcgtcgtc gtccatcgcg
     3361 ccgtcggtga tataaagaag agcacgcttt ccttctttaa gaagtaaatc cgtcaacgca
     3421 tcatagagcg ctgcgctcgc cgtacgcatg ggggtatcgg tcaataaatc atcgcgcaac
     3481 gcacgcttca ggctttgcaa taaattgagg cgcgaatggg taaactcgct ttcatgtcga
     3541 atatcgctgc cgtgcgttac gatttgaaat ccatcttttt cacggatttt tgttaacaga
     3601 tgatctaaca cccacggtag ttgagtttca taatctttca ttttcttaga gcgtgaaact
     3661 aaaatagacg cgaataaaat atcgtcaaac tgctttaagt acgttgtgtc gagattgccg
     3721 accgatgcgt cgttttctaa aaccttgaaa tcctcggctg tcaaattttg taagtaattc
     3781 ggcatgcccg attgacgctc tgaccccatt cccgtagaga cggttacata aacacccatc
     3841 acaggaaatt ttgcggcgct gactttttca acgtgtagat acaacggtgc aaatttctca
     3901 ctctgaggca caaattgcac cacttcgctt gcaccataat cggcaacgta caagttacgg
     3961 aaagcgtcga acgccgcagc ataaggccgt gcgaattcaa caaaccgtcc gtcgttattt
     4021 ttgtatcctg tcaatgcacc taaaatttct cctgcgtcgt tcatacgcac cacgcccgcg
     4081 ctttcgtcgg caattaagaa aacattgtcg gccgcatccc attcgatgtg gcgtggtttt
     4141 tttaattcgg gcggcgaaaa ctcgacnnnn nnnnnnaaca atgctgccgt agcgatcgaa
     4201 gtgcgtgaca cgcgctaaag taggttcggt ggtccaaatt gcttcatcgt tacaccacac
     4261 gccaaagggg gattgcattg catcgacatc ggcggggcgt gtaatttgtg cgataaattt
     4321 tccatcgtcg ctaatgcgtt gcaaacgatt attttctgaa tcggcgacga caaactctcc
     4381 ctcgcggtgg caaagcccag caggagctct aaactctaaa ggcgcactgc ctgttttacc
     4441 caacgcgggt ggattaaaga aataagaaaa aaactgcgct ggtttttttt tcatgcggtg
     4501 gattttatct tctttaaaat cggtaatcca cacttcattt tcattttgcg caaacgccat
     4561 tggataaccc aatttacccg aaacgcggtg ttggttttca aacggttcac cttgcgccga
     4621 atacgaaacc atgttgccca catcgaaagc ccccaacgac aatacggcaa aacgatcgct
     4681 gttaataagc cccacgtacg tcggcctgtt atagcgataa ccttgggtgc gatctaaaac
     4741 gcgtaaaaac tcggttggtt tttcagcggg ggcgtcgtct gaaagtgtaa gcgcgtcaat
     4801 gtggtttttg agcgtcaagt catgcggcat ggcgcgtgcc aacgcttcgt attcgtccag
     4861 cgcatcactt ttctgtcccg agaaataata tgcctcggct aaaaagcggc gtgcccaatt
     4921 aaactcgggg ttgatccgca acgcttgcga aaatttctcc accgctgcat cgaatttacg
     4981 atcttgaaaa aggagccatc ctttttgaaa ctcttcatcg gcttgatttt gccgaatgcc
     5041 caacgcaggt gcaaacacag cgtacgcaag cgaagtcgac aacacaacga aagcggcaat
     5101 agaccatgcg cggtggcgat taaaaaatga agacagcgta tccagcatgt ataatgtgac
     5161 ataatctcga gtaaagatga gtccgtcaag tggtatttag aatacaagtt ctaaaccctc
     5221 aagacatatg ttacactttt tataaagtgt ttaaggtagg tgg
//
LOCUS       DAOMPD010000158         5261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6622_length_5261_cov_3.204764, whole genome shotgun sequence.
ACCESSION   DAOMPD010000158 DAOMPD010000000
VERSION     DAOMPD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6622_length_5261_cov_3.204764"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1162
                     /locus_tag="PLY93_06720"
     CDS             140..1162
                     /locus_tag="PLY93_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP69206.1"
                     /translation="MASWSTYREKGLFGLWLDVLKSGFQIVVLFDSYRYLWDSATGET
                     KKDFDFPENKVFSWESVNGFSLFLFSDTNVESEEQNLWVAYLEYECRKVDTFPSFLPF
                     LPGSFHYDFVTPAPALLFLNGQPGTGKRTLLQLLSLLHGGVLLPESTDSVVCVDGKDG
                     KIAVVPEVALLELAEQVAMEKHILGGGFFWGASVYDLKMLCSREILSAQLTQILMQNR
                     YVLSSLSKRSKEEQMEMASFWRSLYGVTRAGFVANLDYQKSRVLGGDTLSVESILEEG
                     RGLRGVVAEFEKEAILKAHARVGRSQHKIANLLKISRGSLQHKLKKYQLESYASTDAD
                     SEDREE"
     gene            1159..2157
                     /locus_tag="PLY93_06725"
     CDS             1159..2157
                     /locus_tag="PLY93_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69207.1"
                     /translation="MKVDDYIEHQTPGGYLLRVNMRNGTFLAFRNGKPQGWQTAGVET
                     QIHPMLHVIFLGERFHVQTKLDSPLLFFPMERSFVKIPFEYDEEGETRFMRIPQEYEG
                     LDLLPGLGFHFQDYWPNVLPALGDGRRYFITDQEMPRQNIMRIKNKYPRSRLIVFKPE
                     DFMKIRVEREKVEEEGRGRASDLVENFTDNLISQNPVFAARYFLRNLDWRKMYSIPMY
                     SRLKSTEIEIIISFIEKMYKNAPTSHDLVAHQKTLEDLLDYYDGVQALMTSDFARVKS
                     WQRKHQRYKRVVLSSLVATCEFQRKFALLDKNLTYAAFLRDVAQVLKDIPVEQIAA"
     gene            2246..2980
                     /locus_tag="PLY93_06730"
     CDS             2246..2980
                     /locus_tag="PLY93_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69208.1"
                     /translation="MKRLAQAIWFTAALGAGGSLCAYAEPISSNPSIGQFTKKTDKKK
                     NLGFTAFLKKSARSLGGHFIAAIQTVAKQKTILAKKKQLRVAKKSAFRIVIPPKEVDA
                     PHFIERNSGFFTSATTPADFPLVLLTTPVVDYALRVVISTVVRVEIFYDPLEYSSLKV
                     PFLQGAATQGVQRSEQALGNPQDQSQKERVGCLFRASIVAKIVSPLSKKNNFAIDRLR
                     NATQLAHGKLGAEPQLRRNRFRNDRA"
     gene            2919..3773
                     /locus_tag="PLY93_06735"
     CDS             2919..3773
                     /locus_tag="PLY93_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRP69209.1"
                     /translation="MENWAQSLSFVAIDLETTGLNPYRHEIIEIGATAFSLDALGESF
                     SVLIRPEKKMDPKSRAIHQISSEELEQNAVSLEEGIALFMQFLPEGSWVFHNAPFDLA
                     FLKQSFKQTKTPWPQRMYYDHLQLSRKLRPERESHSLAAIKKEVGIDTGKAHRALADA
                     QATALAFIHLLQTEPDIFSSKTKFQKIRRWQRKTEKFEILFPKNYDQIEQYFAGKIRQ
                     ESMIKVDFFDRAGLPQKSFIQPKEIMIFSQNVYIRSVVHPSRQEILIPLNRAVFHDQE
                     LGAVNFVK"
     gene            complement(3810..4217)
                     /locus_tag="PLY93_06740"
     CDS             complement(3810..4217)
                     /locus_tag="PLY93_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69210.1"
                     /translation="MRAVNEIASFIDSPYSPGAIYSRLNKMIKNNQITFNQNFSIQIT
                     PVGYQNLFSYLDTEKHALSITGILYRVACVHIVDEDKINSLGKKRLLLSMINYNQDQI
                     DPKNSSYSSMLKTWTQEFSICLTNIIQKLLTNK"
     gene            4394..>5261
                     /locus_tag="PLY93_06745"
     CDS             4394..>5261
                     /locus_tag="PLY93_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP69211.1"
                     /translation="MKTKVTATVYLVIFLDMLGFGVLIPVVRDLTQALVVHSGLSWPR
                     PEVYMGILMAVYSGSQMLSAPIWGHLSDRYGRKPIFLLSTIGNVFSYVVWIIANDYWL
                     FLFGRILSGITGGNIAIAQSIIADHTTPEERPRAMGLLGAAIGMGFVLGPFLGGWIIN
                     YNHLINTNMIKINQFWSIGVVCLILSIASTIMIIFSHYKEAHKTTLSTVEGFSGVVDA
                     IKSPALRLTYNAHLLSQFSFVIFEVLFAWILQNQYNFDLKETFYFFGIQGLILAFIQG
                     GIYRRVEKKHPPG"
BASE COUNT         1480 a         1002 c         1196 g         1583 t
ORIGIN      
        1 agttcggcgt cttcgcccgt gtagagcaaa ggggggggta gttactccct tttgtggttg
       61 ttttgtctct gtcggatatc cgacacaagt aaaatacata agttgttgac gcacgattaa
      121 aattaagctt tttgtgtaga tggcctcgtg gtcgacgtat cgagagaaag ggttgtttgg
      181 gctgtggctg gacgttttga agtcgggctt tcaaatagtt gttctttttg actcttatcg
      241 ctacttatgg gattctgcaa caggcgagac aaagaaagac tttgacttcc ctgagaataa
      301 agttttttcg tgggagtcgg tcaatgggtt ctctctgttt cttttttctg atacgaacgt
      361 cgagtcagaa gaacaaaatt tatgggttgc ctatttagag tatgaatgtc gtaaggttga
      421 cacattccct agttttttac cttttttgcc tggttcgttt cattacgatt tcgtaacccc
      481 tgcccctgct ctgcttttct taaacggcca gccgggcacg ggtaagcgca cgttgctgca
      541 attgctctct ttactccatg gtggggtgtt gttgccagag tctaccgata gcgtggtttg
      601 tgttgatgga aaagatggta agattgctgt agttccagag gtggctcttt tagagcttgc
      661 cgaacaggtg gctatggaaa aacatatctt gggcggtggt tttttttggg gggcatctgt
      721 gtacgatttg aagatgctct gctcgcgaga aattttgtca gcgcaactga ctcaaatact
      781 tatgcaaaat cggtatgttt tatcttctct ttccaagcgt tcaaaagaag agcaaatgga
      841 aatggcctcg ttctggcgtt ctttatatgg ggtcactcgc gcaggctttg ttgcaaattt
      901 agattaccaa aaaagtcgag tcttgggggg tgacactttg tctgtggagt ctatcctgga
      961 agagggcagg gggcttcgtg gcgttgtggc cgagtttgaa aaagaagcaa ttctcaaagc
     1021 gcatgcccgt gtaggccgtt cgcaacacaa gatagctaat cttctcaaga tctcacgcgg
     1081 ttcgttacag cataaattaa aaaagtatca actggaaagt tatgcctcga cagatgccga
     1141 tagtgaagat agagaagaat gaaagtcgat gactacatag aacaccaaac gccgggagga
     1201 tatttgttgc gtgtcaatat gcgcaatggt acctttttag catttcgcaa tgggaagccg
     1261 cagggttggc agacggcagg cgtggaaacc cagattcacc ccatgctcca tgtgattttt
     1321 ttaggtgaac ggtttcatgt tcaaaccaag ttggattcac ccttgctttt ttttccgatg
     1381 gagagaagct ttgtgaaaat tccatttgaa tatgacgaag agggggaaac tcgttttatg
     1441 cggatacccc aagagtacga agggctggat ttgctcccag ggcttgggtt tcactttcaa
     1501 gattattggc caaacgtgtt gcctgcgtta ggggatgggc gtcgctattt tattaccgat
     1561 caagaaatgc cacggcaaaa catcatgcgg ataaagaata aatatcctcg ttctcgactg
     1621 attgttttta agccagaaga ctttatgaag attcgtgtag agcgagaaaa agtagaagaa
     1681 gagggtaggg gacgagcatc tgatttggtt gaaaatttta ccgacaatct tatttctcaa
     1741 aatcccgtat ttgccgcccg ctatttttta cgtaatctcg attggcgtaa gatgtactcg
     1801 ataccgatgt attcaagatt aaaatcaaca gaaatagaga ttattatttc ctttattgag
     1861 aagatgtaca agaacgcccc tacgagtcat gatcttgtag cgcatcaaaa aacactcgaa
     1921 gacttgttgg attattacga tggcgtacaa gcgctcatga cctctgattt tgcgcgggta
     1981 aaatcgtggc aaaggaagca ccaacgctat aaacgggtgg tgttatccag tttggtggcc
     2041 acttgtgaat tccaaaggaa gtttgctttg ctggataaaa atttaacata cgccgcattt
     2101 ttaagggatg ttgcccaggt attaaaagat atcccagtcg aacaaatagc cgcataagtg
     2161 tttacagagt aaccagatag tataaaaaat ttacaaaaat tcttgacttt ttgactcctc
     2221 cccattgtct atacccaagg tgtttttgaa gagattagct caagcaatat ggtttaccgc
     2281 tgcgttaggg gcgggcggct cgctttgtgc gtacgcagag cctatttctt ctaatccatc
     2341 tatcggacaa ttcactaaaa aaacggataa gaaaaaaaat ctgggcttta ccgcttttct
     2401 gaaaaaatca gcgcgctctt tgggtgggca ttttatcgcc gccatacaaa cagttgctaa
     2461 acaaaaaaca atccttgcta agaaaaaaca gttaagagta gcgaaaaaat cggcatttcg
     2521 gatagttatt cctccaaaag aggtggatgc gccccacttt atagaaagaa atagtggatt
     2581 ttttactagc gcaactacac cagcggattt tccgcttgtt ttattaacta cgcccgttgt
     2641 agattacgct ttaagggtgg taatatctac agtggtgaga gtagagattt tttatgatcc
     2701 tttagaatac tcttcactca aagtgccttt tttacaagga gctgcaaccc agggtgtgca
     2761 gcgtagcgag caagctctgg gaaatcctca ggatcaatcc caaaaagaaa gagtagggtg
     2821 cctgtttaga gcctcgatag tggccaagat tgtttccccg ttatctaaga aaaataattt
     2881 tgcaatcgac cggctacgca acgccacaca gttggctcat ggaaaattgg gcgcagagcc
     2941 tcagcttcgt cgcaatcgat ttagaaacga ccgggcttaa cccatatcgc cacgaaatta
     3001 tagaaattgg cgccactgct ttttcgctcg atgcgttagg agaaagtttt tcagtattga
     3061 tccgccctga aaaaaaaatg gacccaaaat cacgggcgat ccatcaaatt tcgtctgaag
     3121 agctcgaaca aaatgcggtt tcgcttgaag aagggattgc cctttttatg caattcttgc
     3181 ctgagggaag ttgggtgttc cataacgcac cgtttgattt agctttttta aaacagagtt
     3241 tcaaacaaac aaaaacgcca tggccacaac gcatgtatta tgatcacttg cagctttcac
     3301 ggaagctgcg gcctgagcgg gaatcgcact cgttggcagc aataaaaaaa gaagtgggaa
     3361 ttgataccgg caaagcgcac cgtgctctcg ccgatgctca agcaaccgcg cttgctttta
     3421 tacacttatt gcaaacggaa ccagatattt tttcgtcaaa aacaaagttc caaaaaattc
     3481 gacggtggca gcggaagaca gaaaaatttg aaattttgtt tcctaaaaat tatgatcaaa
     3541 tagagcaata ttttgcagga aagatcaggc aagaatcgat gataaaagtc gatttttttg
     3601 atagggcagg gctaccccag aaatcgttta tccaacctaa agaaatcatg atttttagcc
     3661 aaaatgtcta tatacgctct gtagtacatc ccagcagaca agagatttta attccattga
     3721 atagagcagt ctttcacgac caagaattgg gcgccgtaaa ctttgtcaaa taaaggaaac
     3781 atagagtgcg ttcgctttgt ttttgacact tatttattag tcaacaattt ttggatgata
     3841 tttgttaggc aaatgctgaa ttcttgcgtc catgttttaa gcatcgaaga gtaagaacta
     3901 tttttcggat caatttgatc ttgattataa ttaatcatgc ttaataacaa tctctttttt
     3961 ccaagcgagt ttattttgtc ttcgtctaca atatgtacgc atgcgacacg atagagtatt
     4021 cctgtaatcg aaagtgcgtg tttttctgtg tctaggtagg aaaaaagatt ttgataccct
     4081 acaggggtta tctggatgga aaaattttga ttaaaagtaa tctgattatt tttaatcatt
     4141 ttattcaacc gagaatatat tgctcctggt gagtaggggg agtctataaa agaagcgatc
     4201 tcgtttactg cacgcataat ctcgtatcgg gaaatatttc ggggttgctt gtacagtatg
     4261 gcgagaataa gataagtaag cttgtccatg tattctattt agaatattct aaatagaata
     4321 catcctgtca agaaatttca ttaactaaac catctctacc tttactgtgt gtcactatat
     4381 tgaaaaatgt gttgtgaaaa ccaaagtaac agcgacggtt tatttagtta tttttctaga
     4441 tatgctcggg tttggggtgt taatccccgt tgtgcgtgat ttgacgcaag cgttggtagt
     4501 acactcaggc ctgtcgtggc ctcgacccga agtatatatg ggaatcctga tggcagttta
     4561 ttccggctcg cagatgctca gcgcacccat ttgggggcat ttatccgatc gctatggtcg
     4621 aaaacctatc tttttgctct cgactatcgg taatgtgttt agctatgtgg tgtggattat
     4681 cgcgaatgat tactggctat tcttgtttgg acgcatcctc agcggcatta cagggggaaa
     4741 cattgcgatt gcacaaagca ttatcgccga tcacacaaca cccgaagagc gccctcgtgc
     4801 tatgggactt ttaggggcag cgattgggat gggcttcgta ttaggaccat tcttaggtgg
     4861 ttggattatt aactataacc atcttatcaa cacaaacatg attaaaatta atcaattttg
     4921 gagtataggc gttgtgtgtt tgatattgtc tatagcttca actatcatga ttatttttag
     4981 tcactataaa gaggctcaca aaacaacact ttctacagta gaaggatttt caggagttgt
     5041 agatgccatt aaaagcccag cattacgcct cacgtacaat gctcatctat tgagtcagtt
     5101 ttcttttgtc atttttgaag tactctttgc ttggattttg caaaatcaat acaattttga
     5161 cttaaaagaa acattttatt tctttggtat tcaagggctg atattagctt ttattcaagg
     5221 gggaatctac cgccgtgttg agaaaaagca ccctcctgga t
//
LOCUS       DAOMPD010000159         5250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6636_length_5250_cov_3.134167, whole genome shotgun sequence.
ACCESSION   DAOMPD010000159 DAOMPD010000000
VERSION     DAOMPD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_5250_cov_3.134167"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..309)
                     /locus_tag="PLY93_06750"
     CDS             complement(46..309)
                     /locus_tag="PLY93_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRP69212.1"
                     /translation="MVSRSVTIQNESGVHARPAAVFVKMANRFPCDIFVEKDGQRING
                     KSIMGLMMLALTRDARIMITAEGEGEEDAVASLVKLVENNFME"
     gene            complement(311..1249)
                     /gene="hprK"
                     /locus_tag="PLY93_06755"
     CDS             complement(311..1249)
                     /gene="hprK"
                     /locus_tag="PLY93_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRP69213.1"
                     /translation="MRTIKVQEILKDAERLKLRLINHDADLTRTLHEIDVNRPGLALN
                     GFFEHFAHDRIQVFGKGEHAFLIGMEVEQRVRPLERFFHYPIAGIVFTHDNEPPEVFL
                     QFAIKNRCPVFVSELRTSKFINVFSDYLDAELAPQTLLHGTFVEVYGVGILLQGKSGV
                     GKSETALELVQRGHSLVADDAVRIVCRDGSRLYGHVSQTIEHHLEIRGLGIISVSDLF
                     GSGAVRMNKRLDLIVHLEDWDEKKEYDRLGLDDETEEILGVAIAVMTIPVRPGRNIPV
                     LIETAAKNHNLKSRGHHAAKKFQERLARMTANATEN"
     gene            complement(1246..1989)
                     /gene="raiA"
                     /locus_tag="PLY93_06760"
     CDS             complement(1246..1989)
                     /gene="raiA"
                     /locus_tag="PLY93_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRP69214.1"
                     /translation="MDIAYYFKNLPPSDAIKSYAGKKLDKLKERLHHIEGIDVRFSLE
                     RQNQQFEITVRGDATVFHLKKSDKDLYAAIDNAVDVLNNQIDKYRKKFEEKVVVVKEM
                     LIPPSEEAQGDEELEIEMRDAEMKPMDELEAILQLRSHKYRFVMFHQADPKKYGLVTE
                     RNDGNYSLVMRSDEGGFIEKIVKFSDGKIEKRAESVFPVSKFTIAEALDVMRESQRDY
                     LAFLNEETDRLNIIYKRQGGGYLIKRPAA"
     gene            2131..5238
                     /locus_tag="PLY93_06765"
     CDS             2131..5238
                     /locus_tag="PLY93_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69215.1"
                     /translation="MLEYALPVAAAVLVTLFLRRLDKSNVNLKKIKAIAERAERELAE
                     LTLKKKEELKDATIRFDSILINADKYLSALTEKLEQAKSDLTSVENTRQNLRVMDSEL
                     QSLDNTTRSVKDQLKFINESLDKIDQQQKRIKKLQDHVKQVDDEAAHMIDAFQAALKD
                     KSSEILGVLEKKFEEVTSETLKVQSELRDDLMARHVDLKAEIEAGYEQLEGDLKKSAQ
                     DLTLNIENRIQAQIQAVSELQERIFSAEKNLEVTVPDMIRDIKDDILRDMQDQAQKLE
                     TLKLNFSGTEDTMRKKLQAFREELDQQRSILFHEIAAEAERIRDQVSSIDLDTLAKKD
                     EIIKSARGEAQKIQKNIDDFNDQYTRARETLFQEASRREKELSQKLDEIEDLNKAIGV
                     QLQNGRSKGLDEIQNELQKSLTEIKSASRELFSDIEEDILRESTLLREKLSAVQSEVT
                     GIVQKTEERCENLEAKVADISQSTEREFSNYLKELKKAAEKGLNDYREELAERERQFT
                     QGITSLDKGLREKIADADRHFESAQSRLEEIVKDAKNALHKNNIEIEKGRAEIMRDMD
                     MELGDAVQKFRTRIEEKENEFSAKIELNIARFFDMKKGLESSQEALIENLQTEKRRIE
                     NEIRQTNDQQLKLFDQELNERENRYRAQLNSLSVESVSDFKGRVGELMDRLIEMKSET
                     TSTFNEFQENQQEVYAAIVEETRTTSQEIRDLKEALSELKAEGFSVEKSQKAAENLRT
                     LIRELSQKIEQAEGKNQTLGEVYQRVEELKELRTKLETELLMLKDKRDKVDRLEESIN
                     FVLGMQNEIEAKSAGLKKLQTNLDEIEAHYGRLNEERRKADALMQTLLDENRLIQKTI
                     DKLEAQDKHIDSLNEGIQQLNGFYQRVEQRSHLLKEQLDTITDQMIGLHKNEHELQAV
                     QARFMEIEDLMADIEKKKTQIITFNKQFEELRKSMSTSVQQIEKIEQNAESKVRQLSE
                     FMKALDDDRTNKVTLTKTLSPAGVGDKKEMVRQLANFGWTSEEIAGRINLDIHTIETI
                     LSLPS"
BASE COUNT         1662 a         1209 c         1164 g         1215 t
ORIGIN      
        1 ttgaaggcga agtacgcgca atgcgccctc gtcataagga ttttcctatt ccataaaatt
       61 attttcgaca agctttacta aagaagccac tgcatcttct tcgccctcgc cttcggcggt
      121 aatcataata cgcgcatcgc gggtgagtgc aagcatcatc aaccccataa tacttttacc
      181 gttgatacgc tgaccgtcct tttcgacaaa aatatcgcaa gggaaacgat tagccatttt
      241 aacaaaaact gccgctgggc gtgcatgcac gccgctttca ttttgaatcg ttacgctacg
      301 agaaaccatg ttagttctct gtagcatttg cagtcatgcg cgcaagccgt tcctgaaatt
      361 ttttggcggc atggtggccg cgtgatttta agttatggtt cttcgctgcc gtttcgatga
      421 ggacgggaat gttacgccct ggccgtacag gaattgtcat cactgcgatt gcgacgccca
      481 gaatttcttc ggtttcgtcg tccaagccta agcggtcgta ttctttcttt tcatcccaat
      541 cttccagatg cacaataaga tcgagacgct tattcatgcg gacagccccc gaaccaaaaa
      601 gatcgctaac gctaatgata cccaacccac ggatttctaa atggtgttcg atagtttgtg
      661 atacatgacc atagaggcgc gaaccgtcac ggcaaacaat ccgcactgca tcgtcggcga
      721 caaggctgtg cccacgctgc accaactcaa gcgcagtttc gctttttccg acaccgcttt
      781 tgccttgaag caggataccc acgccataca cctcgacaaa agtgccgtgc aaaagggttt
      841 gtggtgcaag ctctgcatcg agataatctg aaaagacgtt aataaactta ctcgtgcgca
      901 attcacttac aaaaacaggg cagcggtttt ttatggcgaa ttgtaaaaac acctcggggg
      961 gttcattgtc gtgggtaaag acgatgcctg cgattgggta gtggaaaaaa cgctcgagcg
     1021 gtcgtacccg ttgttcgact tccataccaa tcaagaaagc gtgctcaccc ttcccaaaaa
     1081 cttggatgcg gtcgtgcgca aaatgctcaa aaaagccatt caaggcaagg ccaggacggt
     1141 tgacgtcgat ttcgtgcaat gtgcgcgtta aatcggcgtc gtggttaatc agacgcagct
     1201 ttaagcgttc tgcgtctttg agaatctctt gaaccttgat ggttctcagg cagctggcct
     1261 cttgatgaga taaccgccgc cttgccgttt ataaataata ttcaagcgat cggtttcttc
     1321 gttgagaaat gccaaatagt cgcgctgcga ttcacgcatt acgtcgagcg cttcagcaat
     1381 agtaaatttt gaaacaggaa acaccgattc ggcgcgtttc tcgatcttgc cgtcgctaaa
     1441 tttgacgatt ttttcgataa agccaccctc gtcggaacgc atcacgagcg aatagttgcc
     1501 gtcattacgt tcggttacca agccatattt tttgggatct gcttggtgga acatgacaaa
     1561 acggtattta tgcgaacgga gctgtaaaat tgcttcgagc tcatccatcg gtttcatttc
     1621 agcgtcgcgc atttcgattt cgagctcttc gtcgccttgc gcttcttcgg aaggggggat
     1681 taacatttct ttcaccacca cgactttttc ttcaaacttt ttgcggtatt tatcgatttg
     1741 attgtttaaa acatcgaccg cattgtcgat agccgcgtac aggtctttat cgcttttttt
     1801 gagatggaac acggtggcgt cgccgcgcac ggtaatttca aattgctgat tttgacgctc
     1861 cagcgagaag cgcacgtcga taccttcaat atggtgcagg cgttctttta gtttatccag
     1921 cttctttcct gcataggact tgatcgcatc ggaggggggg agatttttaa aataataggc
     1981 tatatccatg cgtttaagag actgcttttg tgacccaaag tcaagctaaa aaacacaaat
     2041 atgtggataa aaacaagaca accgccatcg aatcgtaatt tattattttt tgcttgtaga
     2101 cgcaaaggcg agattgtaat gtgacataat atgctcgagt atgccctacc ggtggcagca
     2161 gccgttctcg tcactctctt tctgcgccga ctcgacaagt cgaatgtaaa cttaaaaaaa
     2221 attaaggcta tcgccgagcg agccgagcgc gaacttgccg aattgacgct caagaaaaaa
     2281 gaagaactta aagatgcgac tatccgcttc gactctattt taatcaacgc cgataaatat
     2341 ctttcggcgc tcactgaaaa attagagcaa gccaaaagtg atctcacatc tgttgaaaat
     2401 acgaggcaaa atttacgcgt gatggactca gaactgcaat cgctcgacaa tacaacgcgt
     2461 tcagtaaaag atcagttaaa atttatcaac gagtcgctcg ataaaattga tcaacaacaa
     2521 aagcgtatta aaaaattaca agatcacgta aagcaagtcg acgacgaagc cgctcacatg
     2581 atcgatgctt ttcaagcggc gcttaaagat aagtcgagcg aaattttagg cgttcttgaa
     2641 aaaaagtttg aagaggtaac gagcgagaca ctcaaagtac aaagcgaact gcgcgacgat
     2701 ctcatggcgc gccacgtcga cttgaaggcc gaaattgagg ccggttacga acagttggaa
     2761 ggcgatttga aaaaaagcgc gcaagactta acgctcaaca ttgaaaatcg catccaagca
     2821 caaatacaag cggtgagcga gttacaagaa aggattttct ctgcagaaaa aaatctcgaa
     2881 gtcaccgtgc cggatatgat tcgcgatatt aaagacgata ttttgcgcga catgcaagat
     2941 caggcgcaaa aactcgagac gttaaaactt aatttcagcg gcaccgaaga cacgatgcgc
     3001 aaaaaactgc aagcgttccg cgaagagttg gatcaacaac gttcgatact ttttcacgaa
     3061 atcgccgctg aagcagaacg gattcgcgat caggtgtcga gtatcgattt agacacgctc
     3121 gccaaaaaag atgaaattat taaatcggca cgaggcgaag cacaaaaaat tcaaaagaac
     3181 atcgacgact ttaacgatca atacacacgt gccagagaaa ccctttttca ggaagcatcg
     3241 cgccgtgaaa aagagctttc acaaaaactc gatgaaattg aagacttgaa taaagccatt
     3301 ggtgttcaat tacaaaacgg tcgttcaaaa gggctcgatg aaatccaaaa cgaactacaa
     3361 aaatcgctaa ctgaaatcaa gtctgcttcg cgcgaacttt tcagcgacat tgaagaagac
     3421 atcttacgcg aatcgacact tttgcgcgaa aaattgagtg cggtgcaaag cgaagtgacc
     3481 ggcatcgtac aaaaaaccga agagcgctgt gaaaatttag aagcaaaagt tgcggacatt
     3541 tcacaaagca ccgagcgcga attttcaaat tatttaaaag aactcaaaaa ggcagccgaa
     3601 aaaggattga acgattatcg cgaagagtta gccgaacgcg aacgccaatt cacgcaaggg
     3661 attacaagcc tcgacaaggg tttacgcgaa aaaatcgccg acgcagatcg tcactttgaa
     3721 agcgcacaat cacgccttga agaaattgtc aaagacgcta agaatgcact ccacaagaat
     3781 aacattgaaa ttgaaaaagg tcgtgcagaa attatgcgcg acatggacat ggagctcggc
     3841 gatgcagtgc agaaattccg tacgcgtatc gaagaaaaag aaaatgagtt tagcgcgaaa
     3901 atcgaactca atattgcccg cttcttcgac atgaagaaag gcctcgaatc ctcacaagaa
     3961 gcgctgattg aaaatttaca aacagaaaaa cgccgcatcg aaaatgaaat ccgccaaaca
     4021 aacgaccaac agttgaaatt attcgatcaa gaactcaatg agcgcgaaaa ccgttaccga
     4081 gcacagctca actcactctc cgttgaatcg gtgagcgact tcaagggacg cgtcggcgag
     4141 ctcatggatc gcctcattga aatgaaatcc gaaacgacat cgacgtttaa cgagtttcaa
     4201 gaaaaccaac aagaagttta cgcggcgatt gtcgaagaga cgcgcacaac cagccaggaa
     4261 atccgcgacc ttaaagaagc gctgagcgaa ttgaaagcag aaggttttag cgtagaaaaa
     4321 agtcaaaaag ccgccgaaaa tttacgcacg ctcatacgcg agctttctca aaaaattgaa
     4381 caggccgaag gtaaaaatca aactttgggc gaggtatacc agcgcgtcga agaattaaaa
     4441 gaactacgca caaagctgga aaccgaactt ttgatgctca aggacaagcg cgacaaagtc
     4501 gatcgcctcg aagagagcat caattttgtt ttggggatgc aaaacgaaat tgaagcgaaa
     4561 tcggcgggac tcaaaaaatt gcagacaaac ctcgacgaga ttgaagctca ctatgggcga
     4621 ttgaacgaag aacgccgcaa agcggatgca ttgatgcaaa cgctgctcga tgaaaaccgt
     4681 ttaattcaaa aaacaatcga caaactcgaa gcgcaagaca aacacatcga ttcgctcaac
     4741 gaaggtattc aacagctcaa cggtttttat caacgcgtcg agcaacgttc acacttactt
     4801 aaagaacaac tcgacaccat caccgatcaa atgattggcc ttcacaaaaa cgaacacgaa
     4861 ctgcaagcgg ttcaagcgcg ttttatggaa atcgaagacc ttatggccga catcgaaaaa
     4921 aagaaaacac aaatcatcac tttcaacaaa cagtttgaag agttacgtaa atcgatgtcg
     4981 acgagcgttc aacaaattga aaaaatcgaa caaaatgccg aatcgaaagt gcgtcaactt
     5041 tccgagttca tgaaggcgtt ggacgacgac cgcacaaaca aagtgactct cacaaaaact
     5101 ttgtctcccg ccggtgtggg cgacaaaaaa gaaatggtgc gccaactcgc caattttgga
     5161 tggacatccg aagaaattgc aggccgcatc aatttagaca tccacaccat cgaaacaatt
     5221 ttatcgctgc ccagttaaaa ttcatttttt
//
LOCUS       DAOMPD010000160         5237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6651_length_5237_cov_2.836743, whole genome shotgun sequence.
ACCESSION   DAOMPD010000160 DAOMPD010000000
VERSION     DAOMPD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6651_length_5237_cov_2.836743"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLY93_06770"
     CDS             <1..766
                     /locus_tag="PLY93_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP69216.1"
                     /translation="EGASLFTNIGRAFRAPTAFELFSNGVHEGTSTFENGNASLKEET
                     SLMAELGFKLKSNDFTMNITGYTNKIENYIFSSPTGTFFTDIGGSVYPEYSTTQGNAT
                     IYGIEADAEYTLTEWLAFLGGGDFIRGRNDSLNEALPLIPANRFHAGVTFTGKKIMGI
                     LNPYLQMKARYTARKSELSNDEKRLYSSFADYALLSLKTGGDFAVGSALWTYTLGVDN
                     LLNQKYVDYLSRQKLFTLNPGINVYFKITAPIDLIH"
     gene            771..1583
                     /locus_tag="PLY93_06775"
     CDS             771..1583
                     /locus_tag="PLY93_06775"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRP69217.1"
                     /translation="MKIETNNGYVRLIEGGREQSLWRKSTPDLIQHTYLRTMALATLT
                     TPLYGTRTLSRALMLGLGGGILCRFFEKHFPYVKLDVAEPDTNIIRIAHEHFMLNRSV
                     AIHAVDGRRFLKSVQHRYDIIILDAFDAIYIPHDLMGVEFLRLVKNRLTPSGIFVANT
                     WMSKNLMRHEEATYRYVFSPLFDLRRSPNTDGNRILLFNANGEAKNLQTTIHKRAADA
                     DTRVQYENFYQKRFSLQSAAEKLRIQTVDAPSGEILIDDINADMLRRAAEFN"
     gene            1726..2742
                     /locus_tag="PLY93_06780"
     CDS             1726..2742
                     /locus_tag="PLY93_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRP69218.1"
                     /translation="MIFDWFHSLFSNDMGIDLGTANTLVYVKGQGIVLSEPSVVAIQA
                     STGKVLAVGQEAKRMLGRTPGEIVAIRPMRDGVIADFETVEKMIRYFINKVHNRATLV
                     KPRIVIGVPSGITEVERRAVRESAEQAGARDIFLIDEAFAAALGAKIPVHEPAGNMII
                     DIGGGTTEIAVISLGGMVIADSIRIGGDEFDDAIVKHLRVNHNLVIGERTAEEIKLAI
                     GNAIPEKKTETFELKGRDAGTGLPRTVVIDSVEVRKALKEPIDAIVEATKIALEKTPP
                     ELAADIVERGIVMTGGGCLLKGLDKHLSRETGVPVIRAENPLNCVVEGTGKYLEELKY
                     LKAR"
     assembly_gap    2844..2853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2854..3184)
                     /locus_tag="PLY93_06785"
     CDS             complement(<2854..3184)
                     /locus_tag="PLY93_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69219.1"
                     /translation="MQQCRFVLLIEYTDDEELIDGIGQIEKILPTYDYASRPLRYTNG
                     KNGGFKWVAFKEVKPVAVFSKFAAQVFKLSLKLHALHLAPAYVSLTNVVSAFPHTVPA
                     AILGDRDY"
     gene            complement(3205..4533)
                     /locus_tag="PLY93_06790"
     CDS             complement(3205..4533)
                     /locus_tag="PLY93_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP69220.1"
                     /translation="MKFCLCLVFIFSIFTAALFSLDIELESAINAKDSDKVLAIFRTK
                     KLSEDDLTVLFDFAAQQKEKMRKEYLYAIRDKNTTDATIGKHIYNEIMRLIKENRVED
                     NLPYTLLCLQLAETNKNSDLLYAVAPFLVHPREKLRIEANKIIAIKKDERIYPLIGQL
                     LAGENDIDKIYAMETLTALKDERAVPLLLQQLTHTNKNIRYYALKSLEAIASDKAQYG
                     IIRVAESDASEEVRLKGVEVLRYFKTGPVTSALQKLIGDKNLAVRNLALESALFQKLK
                     AYASTISEQLAQESEPEQKEKLLQGLFVIGSGGGMNGVYKLLKQETDAHLLIGAAYAC
                     NRFNETKCNDLLIARIATEKDESTLVEFLYSLGEFKYKKNLPLFIDILKNAEKSTLVR
                     SAALNAIRAYDGEATVLPLFDIYSLEKDLGLRAQMKETLTDLMRRKLAKL"
     gene            complement(4530..>5237)
                     /locus_tag="PLY93_06795"
     CDS             complement(4530..>5237)
                     /locus_tag="PLY93_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69221.1"
                     /translation="NNLRARIAVLTRIDLDHQNVLGDTLEKIAYEKAGIIHERQHVYS
                     FAQAPSVFAVLKETAAEKNAHLDMLNTTEKNFIDQNRDYARQILKREFSISETQWEAV
                     GEVLQKPIFGRLTQLSATPRIFFDSAHNAVSMHALAAFINTQKETHVQIFINTMTERD
                     LAAMVQILRHTILQKVDFFLFPIFSLHYYVSLPEDSGISTVDDDAIRTLIARKDVLNF
                     FTGSMGIYKELRERFNL"
BASE COUNT         1391 a         1254 c         1171 g         1411 t
ORIGIN      
        1 cgagggcgca tcgctcttca caaatatcgg gcgcgcattc cgcgcgccga cggcgtttga
       61 acttttttct aacggagtcc acgaaggtac aagcacgttt gaaaatggca atgcctcgct
      121 caaagaagaa acatcgctca tggctgaatt gggattcaaa ctgaaatcga atgatttcac
      181 gatgaacata acaggctaca ccaacaaaat tgaaaattat atcttctcaa gtcccaccgg
      241 cacatttttt accgatatag gtggaagtgt ctatccagaa tacagcacca cccaaggcaa
      301 cgcgactatt tatggaattg aagccgacgc tgagtacaca ctcacggagt ggttggcgtt
      361 cttgggcggt ggcgatttca tccgtgggcg taacgattcg ttgaacgagg cattgcctct
      421 catccctgca aaccgttttc acgccggcgt aactttcaca ggtaaaaaaa ttatgggtat
      481 ccttaatccc tatttacaaa tgaaagcccg ctacaccgca agaaagagcg agctttcaaa
      541 cgacgaaaaa agactctact caagtttcgc cgactacgcg ctgctttcgt taaaaacagg
      601 tggtgacttt gctgtcggct ctgcgttatg gacttatact ttgggtgtgg ataacttact
      661 caaccaaaaa tacgtcgact acttaagccg tcaaaaactc tttacgctca accccggaat
      721 caatgtctat tttaagatta ccgcaccaat agatctaata cattaaccta ttgaaaatag
      781 aaacaaataa cggctacgta cggctcatcg aaggcggacg cgaacaatcg ctttggcgca
      841 agagcacgcc cgacttaatc caacacacgt atttgcgcac gatggcgttg gcaactctta
      901 ccacgccgct ctatggcaca cgcaccctta gccgtgcgct catgctgggt ttaggcggcg
      961 gcatcctttg tcgttttttt gagaaacact ttccttatgt aaagctcgac gttgccgaac
     1021 ccgacacaaa catcatccgt atcgcgcacg agcattttat gctcaatcga agcgttgcca
     1081 tccatgccgt cgatggccgc cgtttcttga aaagtgtaca gcatcggtac gacatcatta
     1141 ttctggatgc tttcgatgcg atttacatcc ctcacgacct catgggggtt gaatttttgc
     1201 gccttgtcaa aaatcgatta acccccagcg gcatttttgt ggcaaatact tggatgtcaa
     1261 aaaatctaat gcggcatgaa gaagccacct accgctatgt cttttccccc ctttttgatt
     1321 tacgccgttc tcccaacacc gacgggaatc gcattcttct ttttaacgca aacggcgagg
     1381 caaaaaattt acagacgacc atccacaaac gcgccgccga tgccgacacg cgcgttcaat
     1441 atgaaaattt ttatcaaaaa agattttcac tccaaagcgc cgctgagaaa ctccgcatcc
     1501 aaaccgtcga tgccccctca ggagaaatcc tcatcgacga catcaacgcc gatatgctcc
     1561 ggcgtgcggc ggagttcaac tagaaagtaa atcaccacta aggcgcaatg gtgcccttta
     1621 gtggtgattc ttcgcccctc aagcgcgcta attaaccttt ttagttgtca gcctgtttca
     1681 acacacggca tagacaagaa aacttaccct tatccttgag gttttatgat atttgattgg
     1741 ttccactcac ttttttctaa cgacatgggc attgatttag gcactgcgaa tacgcttgtg
     1801 tacgtaaaag gacagggtat tgtactctct gaaccttcgg tagtggcaat ccaagcttca
     1861 accggtaaag tgctcgctgt gggtcaagaa gccaagcgca tgctcggccg tactcccggt
     1921 gaaattgtgg caatccgccc tatgcgcgac ggggttattg ccgactttga aaccgtcgaa
     1981 aaaatgattc gttattttat caataaagtt cacaatcgcg ccactttagt aaaaccacga
     2041 atcgtcatcg gtgttccttc gggcatcacc gaagtggagc gtcgcgctgt acgcgaatcg
     2101 gcggaacaag ccggtgcacg cgatattttc ttaatcgacg aagcgtttgc tgcggcactc
     2161 ggagctaaaa ttcccgtaca cgaacctgca ggcaacatga ttatcgacat tggcggcggc
     2221 accactgaaa ttgcggtaat ttctcttggc ggtatggtta tcgccgacag catccgtatc
     2281 ggtggcgacg aattcgacga tgcaattgta aaacacttgc gtgtgaatca caatttggtc
     2341 atcggagagc gcaccgccga agaaatcaaa ctcgcgattg ggaatgcaat ccccgaaaag
     2401 aaaactgaaa cgtttgaact caagggccgc gacgcgggca caggtttacc gcgcacggtt
     2461 gtcatcgaca gcgttgaagt gcgtaaagcg ctcaaagaac ccatcgatgc tatcgtcgag
     2521 gctaccaaaa ttgccctcga aaagactccc cccgaactgg ccgccgacat tgtcgaacgc
     2581 ggcattgtga tgaccggtgg tggttgcttg ctcaaaggtc tggataaaca cctctcgcgt
     2641 gaaaccggtg tacctgtaat ccgtgcagaa aatccgctca actgcgtggt tgaaggcacg
     2701 ggtaaatatc tcgaagaatt aaaatactta aaagcgcgct aacgattcgc gccacgcacg
     2761 agttgccata cgtcgtttaa gtagagtata ctgcgcttgt ctttgaataa atcgtcggtg
     2821 aatgcgtacg gtgtaagttt tttnnnnnnn nnngataatc cctatcgcct aaaatcgcag
     2881 cgggcactgt gtgtggaaac gctgacacaa cgttggtaag cgagacgtac gctggcgcaa
     2941 gatgtaacgc atgaagtttg aggcttagct taaacacttg cgccgcaaat ttgctaaata
     3001 ccgcaacggg ttttacttct ttgaacgcta cccacttgaa accgccgttt ttgccattcg
     3061 tgtagcgtag cgggcgcgag gcataatcgt acgtagggag tattttttca atctggccaa
     3121 tgccatcgat gagttcttcg tcgtcggtgt attcaatcaa taaaacaaaa cggcactgtt
     3181 gcattcttat cctcgtttaa tccgttacag ctttgccagt ttacggcgca tgagatcggt
     3241 gagcgtttct ttcatttggg cgcgtaaacc taaatctttt tcgagagaat aaatatcaaa
     3301 aagcggcaac actgtcgcct cgccgtcgta cgcacgaatt gcgttgagag cagccgagcg
     3361 caccaaagtg gatttctcgg cattcttcaa gatgtcgatg aataacggta gatttttttt
     3421 atacttaaat tcgcccaacg aatagagaaa ttcaacgagc gtcgattcgt ccttttccgt
     3481 agcgatgcgc gcgattaaaa gatcgttgca tttagtttcg ttaaatcgat tacacgcata
     3541 cgcagcaccg atgagtaagt gcgcgtcagt ttcctgcttc aataatttat acacaccgtt
     3601 catgcctccg ccagagccaa tgacaaaaag tccttgcagt aatttttctt tttgctccgg
     3661 ctcgctctcc tgcgccagtt gctctgaaat ggtagacgcg tacgctttta atttttgaaa
     3721 aagcgccgac tccagagcca agttgcgcac tgctaaattt ttgtcgccga taagtttttg
     3781 taaagcagag gtgacagggc ctgttttgaa atagcgcaag acttcgacgc ctttaagacg
     3841 aacttcttcg ctcgcgtcgc tttcggcaac gcgaattatc ccgtactgcg ctttatccga
     3901 ggcgattgct tctaaacttt tgagcgcgta gtagcgtata tttttattcg tgtgcgtcag
     3961 ttgttgcaaa agcaacggca ccgctctttc atctttgagc gcagtcagcg tctccatggc
     4021 ataaatttta tcgatgtcgt tttcgcccgc aagcaactgg ccaatgagag gataaatacg
     4081 ttcgtctttt tttatggcga taattttatt tgcctcgatt cgtaattttt cccgtgggtg
     4141 caccaagaac ggagccacag cgtacaagag gtcggaattc ttatttgtct cggccaactg
     4201 caaacacagc agcgtataag ggagattgtc ctcgactcga ttttctttaa taaggcgcat
     4261 aatttcatta taaatatgct tgcctatcgt tgcatcggta gtgtttttat cgcggatggc
     4321 gtagagatat tcttttctca ttttttcttt ttgttgtgcg gcaaaatcaa aaagtacggt
     4381 taaatcgtct tcgcttaatt tttttgtgcg aaaaatagcg agtactttgt cgctgtcttt
     4441 tgcgttgatg gcgctttcaa gctcgatgtc gagagaaaaa agcgcagcgg taaaaataga
     4501 gaaaataaaa accaaacata gacaaaactt cataaattga aacgctctcg cagttctttg
     4561 taaattccca tactaccggt aaagaaattc agcacatctt tacgagcaat caatgtacgg
     4621 atggcgtcgt cgtctaccgt acttattcca gaatcttcag ggagagagac ataataatga
     4681 agtgagaaaa ttggaaacaa gaagaaatct actttttgta aaattgtatg gcgcaaaatt
     4741 tgaaccatcg ccgctaaatc gcgctctgtc atagtgttta taaaaatttg gacgtgcgtt
     4801 tccttttggg tattgatgaa agcggcaagc gcgtgcatag aaacggcatt gtgcgcgcta
     4861 tcgaaaaaga tacgcggcgt agcgctcaat tgtgtcaacc ttccaaaaat cggtttttgc
     4921 aagacctctc ccaccgcttc ccattgtgtt tccgaaatag agaactcccg cttcaaaatt
     4981 tgccgcgcat aatcgcggtt ttgatcgata aaattctttt cggtcgtgtt taacatgtcc
     5041 aagtgcgcat ttttttctgc ggctgtttct tttaaaacgg cgaatacaga aggagcttgt
     5101 gcaaacgaat agacatgctg cctttcgtgg ataatccctg ctttttcata cgcaattttt
     5161 tcaagcgtgt cgcccaaaac gttttggtgg tctaaatcga tccgcgtaag aacggcaatg
     5221 cgcgctcgta aattatt
//
LOCUS       DAOMPD010000161         5207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6697_length_5207_cov_2.558618, whole genome shotgun sequence.
ACCESSION   DAOMPD010000161 DAOMPD010000000
VERSION     DAOMPD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_5207_cov_2.558618"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..890
                     /locus_tag="PLY93_06800"
     CDS             132..890
                     /locus_tag="PLY93_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP69222.1"
                     /translation="MSAAIIAIANQKGGEGKTTTSINLAHGLTLNGMRTLLVDMDPQG
                     NSSGIFTDIDSLSTTVAELFTKKNTVKEIILPTKYKNLDILPAKISLAEVELSALNVD
                     APYVMRDALGSVKNEYDAMVIDCPPSLSIFTINALGAANYVVIPLQAEKFSIDGIKGL
                     QGTIEGIKRRINPELKILGALITQLKSNTVLTKTILPVIQNYFPVFNTSISTGVAVGE
                     SHLSRRSLLDYSSGVKQAKEYAAFVKEVIHGIQK"
     gene            874..1716
                     /locus_tag="PLY93_06805"
     CDS             874..1716
                     /locus_tag="PLY93_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRP69223.1"
                     /translation="MASKNKKLALAADIFEQEIEGIIRKIAISDIQPSVDQPRTHFDD
                     TINNLAESIKTEGLLQPIVVTKEGTHYKIIAGERRYRAARLAGLTEIECRILRKNPKD
                     TYRLAVIENLQRENLNAVDEARAFRKLKIEYGYNDSDLSKIIGKSRNYINEILSVAEI
                     PNTWIEKAKNLGIESKNILIQFAQAVKAGTGESFLTAFESGTVKTVASAKQFNKTQKI
                     PSSQTTTRKATIQDIQCKGDLHNKKWRFTVEFTVSEPGSPDKVAQIEAQLAHFVKERI
                     PKLL"
     gene            2047..2940
                     /locus_tag="PLY93_06810"
     CDS             2047..2940
                     /locus_tag="PLY93_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP69224.1"
                     /translation="MDINNMYLAIMSDILDSGTWAELSPAAKALYPVLCKFSNHTFKP
                     VWPGTDELLRLTGFKTKKSLQEAKRELVDKRLLHIIPGSGRTSSHYYFRFDYKDSKVD
                     IETHRNTVVSRRGIEKHPSADSVDIPLRGDAISPNHINIHINKLTQTTTNNNPDLSSL
                     LKEFFGGERKRGDYREQLVHEMLEKYGSLKMGEAIRIAISKGKDGDIRYLEGILRNRE
                     SNPKAEKNENSFSFENLAPSLKIWQDHLTVMGRFGETWYFSAKSDLSPSFVEEQFRRA
                     GVSIKIVKPNTPRDEDYLAAQ"
     gene            3013..3621
                     /locus_tag="PLY93_06815"
     CDS             3013..3621
                     /locus_tag="PLY93_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69225.1"
                     /translation="MPLMLYTVVFFFLLCIFGLFWFLQNSASNRNPYVSPFFPDHEKT
                     VSTEDLSLIFGVKRIEYLSDRGRWFLFISIMLLIIEFFAVLGMLIENRGQMYWASSIN
                     HGYAIGGFVYLLVSIVAMVLSIRLVSAEKGLSIANPFERLEAWEQKILQADPEQKKRV
                     FLYLSQESSRMEGYFSFTFALIACALTLLTIWIAGWLMQFVR"
     gene            3724..>5207
                     /locus_tag="PLY93_06820"
     CDS             3724..>5207
                     /locus_tag="PLY93_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRP69226.1"
                     /translation="MVALHIERSVPHVTVLLDFEKQIFTNSITYECSAFVLIDILWSN
                     DGKSFYRTHGITHENRGDIKKFSFPLIGARYFQVHLYQEGGLLHKNEVRRFEIGFLSQ
                     AKVRASTELDRLWVADNLTDRREDYGWASKVHEKDEGDTIDVDLGALFFVKEIQLKSI
                     ADEFNFFPVAFQLQLSEDNAVWHTLASEDRFYAGPTTWHAWWFAPTRARYARVQINKH
                     AHYKKGEYQSKILDIAVLAEPEAYSAIGKERDNFTRMASENIAGLVLLSPNNISAPNR
                     VVQSNDSRLRNATTEYRGIMQFARDNEAAQEKAVQGNDSRLRTATEVNPGIVQLAKSG
                     EDRELTAVQGSDVRLKQATQDSVGIVQLAKDGEAKPGVVVQGNDVRLKSASHNSFGLV
                     QLAKDGETTQGKVIQASDFRLRTASQSWPGIIQIAAHSEIASNKAVAADDPRLQEGDE
                     SHKGGVQFARKGEIADLKAVQASDPRLHTATEENIGVVQFARSGT"
BASE COUNT         1609 a         1094 c         1140 g         1364 t
ORIGIN      
        1 aggagcttgt aaaaacttgc ttttttgtta cccactacac gagttggcgt acaggctctc
       61 tgtcggatgt ccgacacata aaataacgcg aatccgcttg acgtggcgta atcgtggtat
      121 cgaatgcact aatgtctgcc gcgatcatcg ctatagcaaa ccagaaaggg ggagagggca
      181 agaccaccac ttccatcaat ttagcgcacg gccttaccct caacggcatg agaaccttgc
      241 ttgtcgacat ggatccgcag ggaaatagct ccggtatttt tacagacatc gactctttaa
      301 gcacaaccgt ggcggagtta ttcacaaaaa aaaatactgt gaaagaaata atcttaccca
      361 ctaaatataa aaacttagac atccttcctg caaaaatcag cctggctgag gtggaactca
      421 gcgctctcaa tgttgacgca ccttatgtaa tgcgtgacgc tttaggctcc gtgaagaatg
      481 aatacgatgc gatggtgatt gactgcccgc ccagcctatc tatttttact atcaacgctt
      541 taggggcagc aaattatgta gtcatcccac tacaggcaga aaagtttagt attgatggaa
      601 ttaaaggttt gcagggcaca atagaaggaa ttaaacgccg cataaatccc gagcttaaaa
      661 tcttaggggc tttaatcacc caactcaaaa gcaacaccgt acttaccaag acaatcctgc
      721 ccgtgattca aaattatttt cctgtattta acacctcaat ttcaacaggg gttgcggttg
      781 gcgaaagcca tctctccaga cgctcgctcc tcgactacag ctctggggta aagcaagcca
      841 aagaatatgc ggcttttgtg aaagaagtca tccatggcat ccaaaaataa aaaactggca
      901 ttagcggcgg acatcttcga gcaagaaatc gaaggcatta ttcgtaaaat cgccataagc
      961 gatattcagc cttccgtaga tcaaccgcgc acccattttg acgacaccat aaacaatctt
     1021 gccgagtcca tcaaaaccga aggactctta caaccaatcg ttgtaaccaa agaaggaact
     1081 cattacaaaa ttatcgctgg tgagcgccgc taccgcgccg cacgccttgc tggtcttacc
     1141 gaaatcgaat gccgtatttt acggaaaaat cctaaagaca cataccgttt agccgttatc
     1201 gaaaatttgc agcgtgaaaa cctcaacgca gtcgacgaag cgcgagcttt ccgcaagcta
     1261 aagatcgagt acggctacaa cgacagcgat ctatcgaaaa ttattggcaa atcacgtaac
     1321 tacatcaacg aaatcctgtc cgttgcggag atccccaaca cgtggattga aaaagcaaaa
     1381 aacttaggta ttgaatctaa aaatatcctt attcaatttg cacaggcggt caaggctggc
     1441 acgggagaaa gctttctcac tgcttttgaa agtgggactg taaaaaccgt cgcttcggca
     1501 aagcaattca acaaaactca gaaaatcccg tcttcacaaa caacgacaag aaaggcaaca
     1561 atccaagaca ttcaatgcaa gggagattta cacaacaaga aatggcgctt caccgttgag
     1621 tttaccgtct ccgaaccagg ctctcctgac aaggttgcac aaatagaagc acaactcgca
     1681 cactttgtga aagagcgcat cccaaagctt ttataaattt cgcttgacgc ccgagaatta
     1741 tgtcacatct ttgaagatga aagaatagaa cttgttggaa tagttatccc gcaataaaaa
     1801 ctttgtgttt ttggatagct aacagaaaaa caaataaaaa acaaaaaagt tctcctcttt
     1861 cagcataaaa ttttaacaac ctttggtttg tgtagtattg tttagaataa aaaaaggggc
     1921 tgctttagaa gcaaccccta aaatttccaa agggaacttg ttggatgatg agacataatc
     1981 catccactca agctgtcaag atttttttgc ttatttctaa gcagacaact gcttaaaaga
     2041 gggataatgg atataaacaa tatgtatctg gctattatgt ccgatatttt ggacagtggc
     2101 acctgggcag aattaagccc tgcagcaaaa gcgttgtacc ctgtgctttg caaatttagc
     2161 aaccacacat ttaaacctgt gtggcctggt acagatgaac tcttacgcct aaccggcttt
     2221 aagacaaaaa aatcactcca agaggcaaaa cgtgagcttg tcgataaacg cttactccac
     2281 atcatccctg gaagcggtcg cacttcctcg cattattact tccgcttcga ctacaaagac
     2341 agcaaggtag atatagaaac ccaccggaat actgtggtat cccgcagagg gatagaaaaa
     2401 cacccttctg cggattcggt agatatccct ctgaggggtg acgctatatc cccaaaccat
     2461 atcaacatcc atatcaataa actaacccaa acgacaacca ataacaaccc tgacttgtcg
     2521 tcgttgttga aagaattttt tggtggtgag cgcaaaagag gggattatcg agaacaatta
     2581 gtgcatgaaa tgttggagaa atacggttca ttaaaaatgg gcgaagcaat tcgtattgcc
     2641 attagcaaag gtaaagatgg ggatatccgt tatctggaag gtattttaag aaaccgagag
     2701 tcgaacccta aagcggaaaa aaatgagaat agtttttcat ttgaaaatct ggcgccatcg
     2761 ctcaaaattt ggcaagacca tttaacagtg atggggcgct ttggcgagac atggtatttt
     2821 tctgcgaaaa gtgatttgtc gccttctttt gtagaagaac aatttaggcg ggcaggggtc
     2881 tctataaaaa ttgtaaaacc aaacacccca cgcgacgaag attatttagc ggctcagtga
     2941 aaaataataa attgattgaa acactatttg tattttcatc atggggcaac ttaaaaagta
     3001 taagggggct ttatgccatt gatgctctac actgttgtgt tttttttcct tctttgtata
     3061 ttcggactat tttggttttt acaaaatagc gcatcgaata gaaatccgta tgtttcacca
     3121 ttttttccag accatgaaaa aacggtaagc acagaagatc tctctttaat ttttggcgtc
     3181 aaacgcatcg aatatctttc ggatcgcgga cgttggtttc ttttcatctc tatcatgttg
     3241 ctcattattg agttttttgc cgtgttagga atgcttattg aaaatcgcgg ccaaatgtat
     3301 tgggcatcgt cgatcaacca tggatatgct atcggtgggt ttgtctattt gctcgtttcg
     3361 attgtcgcga tggttttatc cattcgtctt gtttctgccg aaaaagggct ttctatcgcc
     3421 aatccttttg agcgtctcga agcatgggag cagaaaattt tacaagcaga tccagaacaa
     3481 aagaaaagag tctttcttta cttgagccaa gagtcaagtc gtatggaagg ttacttttct
     3541 tttacctttg cccttattgc ctgtgcatta acattgctca ctatatggat tgccggctgg
     3601 ttaatgcaat tcgttcgata agtgagtaat ctttaatcga tggatttaat tcgttcacaa
     3661 agtgccgaaa ttcggcatgc cacagcttta acttctgcat taaaagttga acggcaaggc
     3721 tctatggtgg cactgcatat tgaacgtagt gtcccacacg taacagtact attagatttt
     3781 gaaaaacaaa tttttacaaa ttcaatcacg tatgaatgtt cggcgtttgt acttatcgat
     3841 atattatggt cgaacgacgg caaaagtttt taccgaacac atggcatcac acatgaaaat
     3901 aggggggata ttaaaaaatt ttctttccct ttaatagggg ctcgatattt tcaagtgcat
     3961 ctttatcaag aaggaggatt gcttcataaa aacgaagtac gtcgttttga aataggtttt
     4021 ctttcgcaag ccaaagtaag ggcaagtact gagttggata gattatgggt tgcggacaat
     4081 ctcaccgaca ggcgggagga ttacggctgg gcgtctaagg tgcatgaaaa agacgaaggc
     4141 gataccatcg atgtcgattt aggcgcactt ttttttgtga aagaaatcca actaaaatcc
     4201 atcgccgacg aatttaattt ttttcccgtt gcgtttcagc tccaactctc agaagataac
     4261 gccgtttggc acacgctcgc cagtgaagat cggttttatg caggtcctac aacgtggcat
     4321 gcttggtggt ttgctccaac gcgggcacgg tatgcgcgcg tgcaaatcaa taagcatgcg
     4381 cactataaaa aaggggagta ccaatctaaa attctagata ttgctgtgct tgccgaaccg
     4441 gaagcgtatt ctgcgattgg taaagaaaga gataatttca cgcgcatggc gagcgaaaat
     4501 attgcagggc ttgttcttct ttctcccaat aatatttctg ctcccaatcg agtggtgcag
     4561 tcgaacgatt cgcgtttgcg taatgcaacg acagaatacc gtggtataat gcaatttgcc
     4621 agggacaacg aagcagcgca agagaaagcg gtgcagggga atgattcccg cttgcgaacc
     4681 gccacagaag tgaatccagg gattgtgcaa ttagccaaaa gtggtgaaga tagagaattg
     4741 acggcggtgc aggggagtga tgtacgatta aaacaagcca cacaagacag tgtagggatt
     4801 gtacaacttg ctaaagatgg agaagcgaaa cctggtgttg tagtccaagg aaacgatgta
     4861 cggttaaagt cagcaagcca caattctttt ggattggttc aactcgcaaa agatggagag
     4921 acaacacaag gaaaggtaat tcaagctagt gactttcgct tgcgcacagc atctcaaagt
     4981 tggcctggta tcattcaaat agcagcgcat agtgaaattg catccaataa agctgtggcg
     5041 gcagacgatc ctcgtttgca agaaggggat gaatcacaca aaggtggagt gcaatttgct
     5101 cgaaaaggtg agattgccga tttaaaagct gttcaagcaa gcgatccgag actgcataca
     5161 gctaccgagg agaatatagg agtggtgcag tttgcccgca gcggtac
//
LOCUS       DAOMPD010000162         5194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6716_length_5194_cov_2.950574, whole genome shotgun sequence.
ACCESSION   DAOMPD010000162 DAOMPD010000000
VERSION     DAOMPD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6716_length_5194_cov_2.950574"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..749
                     /locus_tag="PLY93_06825"
     CDS             120..749
                     /locus_tag="PLY93_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRP69227.1"
                     /translation="MPFNSMAYKKETFFIRYIRRIEQRKFCRIFSVAIFSFVLTTSLH
                     ADAAYESLKNNVKGFYSSFRANFTISSGSGMQSGKIMYQYPGKLRIETSTGVIATNGQ
                     HLWVHNKSSPLCARQDVGGVGGGLLGILGSYEGTVQGSTYIFKKKGAHFDEIVVQTGG
                     GTVRSVRMKHGDETITYTFSGMAIDTGISGAMFNYRPSGATIVENPLNN"
     gene            758..>2509
                     /locus_tag="PLY93_06830"
     CDS             758..>2509
                     /locus_tag="PLY93_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69228.1"
                     /translation="MQEFPDGKVGSQVSGKRILTRSLKVVGKILTYGLALSVVGVVVG
                     YNYVMRPQFLEPLIVKQFATHTNGTIELKLEQTSLFRGFKFKNVIVRAPEEYSRTPIL
                     QAGEINLLYNVFGFFRGKFGVHEISLKNINIFIEQKKDVMNVQALLKPGKEKEPKEEI
                     EKPESESSVISWFFDVQVFVRFALENFNFTLDARDASNKVKQYAHLKNFHFNFSVLSK
                     DFNSIDTKDTASLVSLLNALVVELNPQKTIDVSYQGPAARITSPIDLSWLLFYDGMAK
                     KPEFMSRMYVGQEKIPVALGARVAQNLSFLLQHKIEYNAKEDKLNVDHFLLKFLGDTV
                     LSFSGSGEKLLTTKRKLQIQTHASRLNLGEVYRVVSTLTGKREPVYSGFFSVKPTRVS
                     IDGNAIEDAGGLKLERVYARTRGLSISIPYLTFDHSASIVQGLKPLPVKRAEAKLAAS
                     LNGAPLNFTALLEEKKTAIDFSLRGFNITPFARSQAEGLVSTTLSAVGESPQALSLTL
                     RVFSPQLYYFLDRGRSGLNRFDFTVKGTTRSSENFKQNSIYLPTILLTNKDKEYNSAL
                     FLKSRLQLEKAQTTKIVY"
     assembly_gap    2510..2519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2520..3675
                     /locus_tag="PLY93_06835"
     CDS             <2520..3675
                     /locus_tag="PLY93_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69229.1"
                     /translation="IDEFVLGFKQLLNTLPAALRENLGAQLTLLNPGRELKVSGETHV
                     TVAGETVIAHETKWALPDIHIDDIRVKAKARLVPVATYIDEFSITGLKGALRVGANGA
                     LKKGSEVVVDEKTKRQTRVATTIPNLRYSVELSQKKENEILEGTHLVGAFTLSGTARG
                     NIVDGKIGIDDLSVRNPKVKIQNVNLDFPFKHDLKLNKTLNLRAGNKERIIKNYNFNR
                     AYNFSIAGIDISDPNNKREWLKIVYPRGNYPAVGASMEYKDNVFVMPVMQMYTLNGVV
                     TISDTLFNLGRLRPKEMEYSSTIQIKDIDLKQLLPSDKAETITDGKLRIDMLFTGNRL
                     DKPIENLNGYISIFRIGPEFADTVMKAVMPKSSSIVTTLAANTTIPKNGH"
     gene            3665..3850
                     /locus_tag="PLY93_06840"
     CDS             3665..3850
                     /locus_tag="PLY93_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69230.1"
                     /translation="MDIEFRDGFVYSDIPMEKKIFGYFVPSPEEIKNRRINIPEFFQR
                     ISDEASVYRSQPSSSGE"
     gene            3851..>5194
                     /locus_tag="PLY93_06845"
     CDS             3851..>5194
                     /locus_tag="PLY93_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69231.1"
                     /translation="MGAFTYIPEFDYDSVKASDSAECIKHLLPFKGGFLKGLEAHLVN
                     ADNIQKFLSSAPTETRKNIAKNQKVIDGALVSILRLYSHVEEELSIRSLDMAAQRAYY
                     NHFPNTEIEDAKMEFCKLVRAYEVIKEFNTEVIDQWWKIAQVFDTFNVPMGENTFLEQ
                     LLVYRVYPRIDLCGLTNLLLRRLAYLLRISSEETIAGKISVHAEYTSLIHYSYSVIFR
                     DDLSEIIENNTVSKSATKVDSVRRTEAAREARDTAVKSPYLLDTRGSEQFNQTHAYIL
                     EINEARFSLEEAKIKRSMYIETHLGAEEEALRQDLIRKFISAARNKDRLGEYKAFLVN
                     YFQFTRDTLMMQQPNVAENDHNLFLYHFGPFFFARICGHFMRIGRTGYVHRHLSRNQM
                     ARELPFEYVKFILKDWWDENIYIALPRTERNSVIAYERILATVRSEWQEHQLRLLS"
BASE COUNT         1458 a         1118 c         1218 g         1390 t
ORIGIN      
        1 cgcggcgcca gataagcctg tgcgtggagt ggggataaaa aacaaatagc tatgcaaaag
       61 cacagcaacg cgatatggaa tcttgcttta cagcgaatca taacgatata gagaaccgta
      121 tgcctttcaa ttctatggct tacaaaaaag aaaccttctt tattaggtat atacgacgta
      181 tagagcaaag aaaattctgt cggattttct ctgtcgcgat tttttcattt gtgttgacta
      241 catccttgca cgccgacgct gcgtacgaat cgcttaaaaa caatgtcaaa ggattttatt
      301 cgtcgttccg cgctaatttt acgattagct cgggcagcgg tatgcaaagc ggtaaaatta
      361 tgtaccaata cccgggcaag ttacgtattg aaaccagcac cggagttatc gccaccaatg
      421 gtcagcacct ttgggtgcac aataagagtt cgccgttgtg tgcacggcaa gatgtgggcg
      481 gcgttggggg cggtcttttg gggattctcg gttcgtacga gggtacagtt caagggagca
      541 cttatatttt caaaaagaaa ggcgcacact tcgatgaaat tgtggtacaa accggtggtg
      601 gcacggtgcg cagtgtgcgc atgaagcacg gcgacgaaac aattacttat acgttctccg
      661 gtatggcgat cgacaccggt atctcagggg cgatgtttaa ctaccgccct tcgggagcga
      721 cgattgtcga aaacccgttg aataattaac agaaactatg caagaattcc ccgacggtaa
      781 agtgggctcg caagtatctg gaaagaggat acttacgcgt tcgctcaaag tggtaggtaa
      841 aattttaacc tatggactgg cgttatcggt tgtgggggtt gtggttggct acaattatgt
      901 catgcggcct cagtttttag agccattgat tgtaaaacaa tttgctacgc ataccaatgg
      961 cacgattgaa cttaaactcg aacagacctc cctttttcgc ggattcaaat ttaagaatgt
     1021 gattgtgcgt gcacccgaag agtattccag aactcccatt ttacaagcgg gtgagattaa
     1081 cttactctac aatgtatttg gtttttttcg cgggaaattt ggcgtccatg aaattagctt
     1141 aaaaaatata aacatcttta tcgaacaaaa aaaagatgtg atgaatgttc aggcgctcct
     1201 aaaaccagga aaagaaaaag aacctaaaga agaaatcgaa aaaccagaga gcgagtcgtc
     1261 ggttatttct tggttttttg acgtacaggt gttcgtgcgc tttgcgcttg aaaattttaa
     1321 ttttacgttg gatgcgcgcg acgcctcgaa taaagtgaag caatacgcgc acttgaaaaa
     1381 ttttcatttt aatttctctg tgctcagcaa agattttaac agcatcgata caaaagatac
     1441 ggcatcgttg gtttccctgt tgaatgcgct cgttgtcgag ttgaatccgc aaaagaccat
     1501 cgatgtttca taccaggggc ctgcagcgcg cattacatcg ccgatcgatt tgtcgtggct
     1561 tcttttctac gacggcatgg caaaaaagcc ggagttcatg tcgcgcatgt acgtgggtca
     1621 agaaaagatt ccggtggcgt tgggcgcgcg cgtggcgcaa aatttatctt ttcttctcca
     1681 acataaaatt gagtataatg caaaagaaga taagctcaat gtcgatcatt tcttgctcaa
     1741 gttcttaggc gatacggtgc tctcgttctc tggttcgggt gaaaagcttt taaccacaaa
     1801 gcgtaaattg caaattcaaa cccacgcatc acggctaaac ttgggggagg tgtaccgtgt
     1861 ggtgtcgacg ctcacgggta agcgtgagcc ggtgtattcg ggtttttttt cagtaaagcc
     1921 cacccgtgtt tcaatcgatg gcaacgctat cgaagacgct ggaggattaa aactcgaacg
     1981 ggtttatgcg cgcacgcgtg ggttgagtat ttcgataccg tacttgacgt ttgatcattc
     2041 ggcgtcgatc gtgcaaggtt tgaagccatt gcctgtcaaa cgcgccgaag caaaactcgc
     2101 tgcctcgctc aacggcgcgc cgctcaattt cactgcactt ttagaagaga aaaaaacagc
     2161 gattgatttt tcgttgcggg gttttaatat cacaccgttt gcacgttcgc aagccgaagg
     2221 tttagtcagc acaactttga gcgcagtggg ggagtcccca caggcgttat ctttgactct
     2281 gcgcgttttt tcaccgcagc tttattactt cctcgatcgt ggccgttctg gtttgaaccg
     2341 gtttgatttc accgttaaag gcacgacaag aagtagcgaa aactttaagc aaaactctat
     2401 ttacttaccg acaattcttt taacgaataa agataaagag tataactctg cgttattttt
     2461 gaaatcgcgt ttgcagcttg aaaaagcgca aacgacaaaa attgtttatn nnnnnnnnnc
     2521 atcgatgagt ttgttttggg gtttaaacaa cttttgaata cattacccgc tgctttgcgt
     2581 gaaaatttag gcgcacaact tacactcttg aatcctgggc gcgagctcaa ggtctccggg
     2641 gagacgcatg tcacagtcgc aggcgaaacc gtgattgcgc atgaaaccaa atgggcgcta
     2701 cccgacatcc acatcgacga catccgtgta aaagcgaaag cgcgtttagt gccagtcgca
     2761 acgtatatcg acgagttttc catcaccggt cttaaaggcg cgttgcgcgt tggcgcaaac
     2821 ggtgcgctca aaaaaggcag cgaggtagtg gtggatgaaa aaacgaaacg acaaactcgt
     2881 gtggcgacaa cgattcccaa tctccggtat agcgtcgaac tttctcaaaa aaaggaaaat
     2941 gaaattttag agggaacgca tcttgtgggc gctttcaccc tatcaggtac tgcgcggggc
     3001 aatatcgtcg atggcaaaat tggcatcgac gacttgagcg tgcgcaatcc caaagtgaaa
     3061 atacaaaacg ttaatttgga ttttcccttt aagcacgact taaaattgaa taagacattg
     3121 aacttacgcg ctggcaataa agagagaatc attaagaact ataattttaa tcgtgcgtat
     3181 aatttttcca ttgccggtat cgatatctcc gatcccaaca acaaacgcga gtggctcaag
     3241 attgtgtacc cgcgcggcaa ctatcctgcg gtgggcgcgt cgatggaata taaagataat
     3301 gtttttgtga tgcctgtcat gcagatgtat acgttgaacg gcgtggtgac catttcagat
     3361 accctcttta atttaggtcg cctgcgtccc aaagagatgg agtactcctc gacgatacaa
     3421 attaaagaca tcgacttaaa acagcttctc cccagcgata aggcggaaac catcaccgac
     3481 ggtaaattgc gtatcgatat gctttttacc ggcaaccgct tagataagcc aattgaaaat
     3541 ttgaacggct atatttccat ttttagaatt ggtcccgagt ttgccgatac ggtgatgaaa
     3601 gcggtgatgc ccaaaagcag cagtatcgtt accacgcttg cggccaatac aacgatacct
     3661 aaaaatggac attgaattcc gcgacggttt tgtctattca gatattccca tggagaaaaa
     3721 aatattcggt tacttcgtgc cttctccgga agaaattaaa aaccgccgca tcaacattcc
     3781 agaatttttc cagcggatat ccgacgaggc aagcgtgtac cgctctcaac cttcatcttc
     3841 aggggaataa ttgggcgcct tcacgtatat acccgagttc gattacgatt cggtaaaggc
     3901 ctcagattcg gcagagtgca ttaagcatct tttgccattc aagggcggct ttctcaaggg
     3961 gttagaagcg catcttgtaa atgcggacaa tatacaaaaa tttttatcgt cagcgccaac
     4021 ggagacgcgt aagaacattg cgaagaacca aaaggttatt gatggtgcgt tggtttctat
     4081 tttgcgcctt tacagccatg ttgaagaaga actttcaatc cgctccctcg acatggcggc
     4141 acaacgagcg tactacaacc attttcccaa cacagaaatt gaagatgcaa aaatggagtt
     4201 ttgtaagctt gtgcgtgctt acgaggtgat taaagagttt aacaccgaag tgatcgacca
     4261 gtggtggaaa attgctcaag tctttgatac ttttaacgta ccgatgggag aaaatacatt
     4321 cttggagcaa ttactcgtgt accgagttta tcctcgtatt gatttgtgtg ggttaaccaa
     4381 tttactcttg agacggttgg cgtatcttct acgcatttct tcagaagaaa ccatcgccgg
     4441 aaaaatctcg gtacacgcag agtatacgag tttgatccac tattcgtatt cagtaatttt
     4501 tagagacgac ctatccgaaa ttattgaaaa taacactgtt agcaaaagcg ccaccaaagt
     4561 ggatagcgtt cgccgtactg aagcggcacg agaggcgcgc gatactgcag taaaatcgcc
     4621 gtatctactc gacacccgag gttcagaaca gttcaatcaa acgcacgcgt atattttaga
     4681 gattaacgaa gcgcgctttt cactcgaaga agcaaaaatt aagcgcagta tgtatatcga
     4741 aacccattta ggcgctgaag aggaagcgct gcggcaagat ttaatccgta agtttatttc
     4801 tgctgcgcgc aacaaagatc gcttgggtga atacaaggcg ttcctcgtta attattttca
     4861 attcactcgc gacacgctga tgatgcaaca acccaatgtc gcagaaaacg atcataattt
     4921 atttttatat cattttggtc cttttttctt tgcgcggatt tgcggtcact ttatgcgtat
     4981 cggtcgcacc ggttatgtcc accgccattt aagtcgcaac caaatggcgc gcgagttgcc
     5041 gtttgaatac gtaaagttta tacttaagga ttggtgggac gaaaacattt atatcgcatt
     5101 gccgcgcacc gaacggaaca gcgttatcgc ttatgaacgt atcttagcga ctgttcgtag
     5161 cgaatggcaa gagcatcagc tacggttatt gtcg
//
LOCUS       DAOMPD010000163         5188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6727_length_5188_cov_2.723748, whole genome shotgun sequence.
ACCESSION   DAOMPD010000163 DAOMPD010000000
VERSION     DAOMPD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6727_length_5188_cov_2.723748"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="serS"
                     /locus_tag="PLY93_06850"
     CDS             <1..1158
                     /gene="serS"
                     /locus_tag="PLY93_06850"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRP69232.1"
                     /translation="EAADARRRIIREVEALAAERNAESKRLGELKAQGKDAEFDALRE
                     RMKGVAERLKVVKEEESAIEENFQNLLSRLPNMLDDQVPDGKDETQNVVVKKVGEIPH
                     FDFDPKPHYEIAEAENLVDFERGVKLSGARFYVYNDKIARLERRLSNFMLDTHAESGY
                     SERSVPIVVKDECMFGTGQFPKFADEYYRMEKDGLSLIPTGEVPLTNLYTDEILNDVD
                     LPIKLMAFTPCFRREAGSAGKDTRGLIRVHQFYKVELVKFVRPEDSEKEWRSLTADAE
                     RILQKLGLTYRVLALCAGDMGFTSSITYDLEIWMPGLKRWLEISSCSNFKDFQARRAK
                     IRYKNASGKNEYVHTINGSGVAAGRLIAALLEYHQNADGSVDWAALEAKLV"
     gene            complement(1181..2086)
                     /locus_tag="PLY93_06855"
     CDS             complement(1181..2086)
                     /locus_tag="PLY93_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69233.1"
                     /translation="MKNWGCTLCDGLGYRVVPQTVHRQAAYTLEPCSCVTILCASCPG
                     DQKSPWLYLDESTGKIRACACKVPRDNLAKTRSLFTASNIPKKYRYHRLKEFNLQPLY
                     PNPTHAPELEQAKRAATIALTAAFDRARHFIDAQKKISQSNDPISSSDLKGLFLIGPP
                     GTGKSLLAAMILNELILTARLSCRYVKISRDFFQQLRSTFNNDSSTYGKTETVFNEIA
                     QQDILVIDDFGIQSDSEWEQRMLYDLVDARYEADLPTIITSNIELEAVKNLFKGRIYS
                     RFTEMLHIVEFFAVRDYRQDMQSFL"
     gene            complement(2451..3548)
                     /locus_tag="PLY93_06860"
     CDS             complement(2451..3548)
                     /locus_tag="PLY93_06860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP69234.1"
                     /translation="MYTTNYGLMERHVEFDELENHLKALDGKISVQDFSTLTTLVKVL
                     TEKHNELISDFNDQKLLYETTLEHSTEIENEISAKNDQITTLMEQMKKYLSSQLYDLI
                     VHGNISNDTQQNQRKKLSIFFSDIVGFTDLTDSVEPELLATLLNEYLDEMSKIALKYG
                     GTIDKFIGDAIMIFFGNSENADDRKEAQNCVLMALEMQSMMKRIRDSWKNQGINHHLR
                     IRIGINTGYCTIGNFGSSERMDYTIIGGQVNAAARLEHLSENGGILISGSTYLLVEDI
                     VEAELRGQIQVKGIQRPIEVYQVMGRKNKDEASAVLIKENKNGFDLGHIQFRAGLTSR
                     LERDEYIAALQKALELLQLSPEIAPKHLVDK"
     gene            3629..4447
                     /locus_tag="PLY93_06865"
     CDS             3629..4447
                     /locus_tag="PLY93_06865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP69235.1"
                     /translation="MPTNTSDGLYKENLYASPLDLSEELPRLAAQARLLFAAQKFFIE
                     GLKLPSPNTDAQFIDLGAGPGIHLSLVKTLFPQYKFTGIELSDKLFQYAKLNFPEINW
                     QQGSIYQIPTADAAFDIAQSSFIFIHLREPTVALKEIYRVLKPGGTFFVLDVDDSTFK
                     GMPEMETMVREHDKIYEADRTIMSRMKAMAEAVGFTQIKEEFVHVDNTGKEDAPSIEY
                     PHFHLGKMTFWSMFAFMGQRSEIAQHYELAHSQYLASAQNKCEISIAFHAYTKK"
     gene            4450..>5188
                     /locus_tag="PLY93_06870"
     CDS             4450..>5188
                     /locus_tag="PLY93_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69236.1"
                     /translation="MEANKISSGANLLSEAEEMLVAPAKNVKIRLLIIFNFIVSLPVL
                     VFNTLTLVKFGNFANPARDWYLYPTLHLIMLSSGVYNLSQQIKGTSGNPIVVRLSTWA
                     TVLILQLVSIVTVQANSNLPLSFLWDVSISLVLIYLTAVMLNRWAALVYAAVAFSNML
                     FLASRLGFDFEYVLQAPPGFPFQLPAKGVLQFVILWGLLMSISVLVAFSEGGVIGKIL
                     KAIPQVVNKIRDAGDTQRHLEMANMRMG"
BASE COUNT         1314 a         1108 c         1280 g         1486 t
ORIGIN      
        1 gaagccgctg acgcacggcg taggatcatc cgcgaagtag aagcgcttgc tgccgaacgt
       61 aacgccgaat caaagcgttt gggcgaactt aaagcacagg ggaaagacgc cgaattcgat
      121 gcgttacgcg aaagaatgaa aggcgttgcc gagcgcctca aagttgttaa agaagaagaa
      181 agcgccattg aagaaaattt tcaaaatctt ttgtcgcgcc tacccaacat gctcgacgac
      241 caagtccccg acggtaaaga cgaaacccaa aatgtggttg tgaagaaagt aggggagata
      301 cctcatttcg atttcgatcc gaaaccgcat tacgaaattg ccgaggccga gaatcttgtc
      361 gattttgagc gcggagtgaa actttcgggc gcgcgttttt atgtgtacaa cgataagatt
      421 gcgcgccttg aacggcgtct atctaacttt atgctcgaca cgcatgcaga atctggatac
      481 agtgagcgca gcgttcctat tgtcgttaaa gacgagtgta tgttcggtac gggtcagttt
      541 cccaagtttg ccgacgaata ctaccgcatg gaaaaggacg gtttgtcgct tatacctacg
      601 ggtgaagtgc cgctcactaa tttatatacc gatgagattc taaacgatgt cgacctaccg
      661 attaaactca tggcgtttac gccatgcttt cgacgcgaag cagggagcgc gggtaaggac
      721 acacgagggc ttattcgcgt acaccaattt tacaaagtag agcttgtaaa gtttgttcgt
      781 cctgaagatt ctgaaaaaga gtggcgttcg ctcaccgccg acgccgagcg cattttgcaa
      841 aagttggggc tcacctaccg cgtgcttgct ctttgcgcgg gagatatggg atttacttcg
      901 tcgattacct atgaccttga aatttggatg cccggtctca aacgttggct tgaaatttcg
      961 tcgtgttcga actttaaaga ctttcaagcg cggcgggcaa aaatccgcta taaaaacgca
     1021 agcggtaaga acgaatacgt tcataccatc aacggctcgg gtgtcgccgc aggacgcctg
     1081 atcgcggcgc ttttggagta tcatcaaaat gccgatggca gcgtcgactg ggcggcgctc
     1141 gaagcaaagt tggtataaaa aagtcgggtt ttttgagtat ttacaaaaaa ctctgcatgt
     1201 cctgccgata atcgcgcacg gcaaaaaact caacaatgtg gagcatctcg gtaaagcgag
     1261 aatagatgcg tcctttgaaa agatttttca ccgcttcaag ttcaatgttc gatgtaataa
     1321 ttgttggtag atcggcctcg taacgggcgt cgacaagatc gtagagcata cgctgttccc
     1381 attcgctgtc ggattgaata ccgaagtcgt cgataaccag aatatcttgc tgtgcgattt
     1441 cattgaatac agtttcagtt ttgccgtacg tgctcgaatc gttgttaaat gtcgatcgta
     1501 actgttgaaa aaagtcacgc gatattttta cataccgaca tgaaagtcga gcagttaaaa
     1561 taagttcatt gagaatcatc gctgccaata acgatttacc cgtaccaggt ggtccaatga
     1621 gaaaaagacc tttgaggtcg gaggatgaga tggggtcgtt gctctggctt attttttttt
     1681 gcgcatcgat aaagtgccgc gctctatcga atgcggcggt gagtgcaatc gttgccgcac
     1741 gttttgcttg ttccagttcg ggtgcatgcg ttgggttggg atagagaggt tgcaggttaa
     1801 attctttgag acggtggtag cggtattttt tggggatatt ggaagcggta aatagagaac
     1861 gtgtttttgc taaattatct cgtggcactt tacacgcaca cgcacgaatt ttacccgttg
     1921 attcgtcgag gtagagccat ggactttttt gatctcctgg acagctcgcg catagtattg
     1981 ttacacaact acatggctca agcgtgtacg ctgcttgccg atgcacggtt tgcggtacaa
     2041 cgcggtaccc taaaccgtcg cagagagtgc atccccaatt tttcattctt taccacccaa
     2101 tcgcaaaatt tgggtagagg gtaaacgatt tgaagcatac gaacatcatt ttatatttag
     2161 tgtaaatctg gttgcgagcc tgtgcgccaa ctcgcgcagc atgtatgaag aggtaatttt
     2221 tttgcaaatt tttgtacgcg gttcgcttaa aaacgcgaaa ttttgcacaa attcaggcgt
     2281 cttcgcgccg tgcagagcaa gtttgcacaa tttgccgtcg gcctcacggc gttacacgcc
     2341 gctttgacgg aggaatgtgt gcaaagtggc atacaaaaat ttgtaaaaaa attgtctcag
     2401 cgagttgatg cacaggctct taagagaaat actgtacgtt gggctacagt ttatttatcg
     2461 accaagtgct tgggcgcgat ctcgggagat aactgcaaaa gttccaacgc tttttgcaat
     2521 gccgcaatgt attcgtcgcg ttcgagtcgg ctagtgagcc ctgcacggaa ttgaatgtgc
     2581 cccaaatcga atccattttt gttttctttg atgagaacag ccgacgcttc atctttgttt
     2641 tttctgccca tgacttgata gacttcaatg gggcgttgga ttcctttgac ttggatttga
     2701 ccacgtagtt ccgcttcgac aatgtcctca accagtagat acgttgaacc gctgatgagg
     2761 ataccaccgt tctcgctcaa gtgttccagc cgtgcagcgg cgttcacttg accgccgatg
     2821 atagtgtagt ccatccgttc gctactgccg aaattgccga tggtacaata accggtattg
     2881 atgccgatgc ggatgcgcag gtggtggttg atgccttggt ttttccaaga gtcgcggatg
     2941 cgtttcatca tcgattgcat ctcaagcgcc atgagaacgc agttctgtgc ttcttttctg
     3001 tcgtcggcat tttcagagtt accaaagaaa atcataatgg cgtcgccgat aaacttgtcg
     3061 atggtaccgc catacttaag cgcaatttta ctcatttcgt ctaagtactc gttgaggagc
     3121 gtggctaaaa gttcgggctc gaccgagtcg gttaaatcgg tgaaccccac aatgtctgaa
     3181 aaaaagatag aaagtttttt gcgttggttt tgttgtgtgt cgttggatat attcccgtgt
     3241 acgattaagt cgtagagttg cgatgagaga tactttttca tctgttccat gagcgtggtg
     3301 atttgatcgt tctttgcgct gatttcattt tcaatttcgg tcgaatgttc gagcgtcgtt
     3361 tcatagagta atttttggtc gttgaaatcc gatataagct cgttgtgctt ttcggtgagt
     3421 acttttacga gtgtggtgag cgtgctaaaa tcttgcaccg atatttttcc atcgagcgct
     3481 ttgaggtggt tttcgagttc gtcgaattct acgtgtcttt ccatgaggcc ataatttgtc
     3541 gtgtacatac gaaggaatgt cttttttcct tgtagtcgta tagtacaata cttagtatgg
     3601 tttttacttt tgtacaagga acataactat gccaacaaat acaagtgatg gtctctataa
     3661 agaaaatcta tacgcttcac cgcttgattt atccgaagag ttgccgcgct tagcggcgca
     3721 agcacgctta ctttttgccg ctcaaaaatt ttttatcgaa ggattaaagc tcccttcccc
     3781 caatacagat gcgcagttta tcgacttggg agcaggacca ggcattcatc tctcgcttgt
     3841 taaaacactt tttccgcagt ataaatttac cggcattgag ctctcggata aactctttca
     3901 atacgcaaag ctcaactttc cggaaatcaa ttggcaacag ggtagtattt atcaaatccc
     3961 cactgccgat gcagcgtttg acattgcaca atcgagtttt atctttattc acttgcgcga
     4021 gcctacggtt gcactcaaag aaatttatcg cgtcttaaaa ccagggggga cgttctttgt
     4081 gctcgacgtc gacgactcga cattcaaagg tatgccagag atggaaacga tggtcagaga
     4141 acacgataaa atttatgagg cagaccgtac catcatgtcg cggatgaagg caatggcaga
     4201 ggcagtggga tttactcaaa ttaaagaaga gtttgtgcat gttgataaca ctggcaaaga
     4261 ggatgctccc tctatcgaat atcctcattt tcatttgggt aaaatgacct tctggagcat
     4321 gtttgcattc atgggacagc ggagcgaaat tgcacaacac tatgaactcg cgcattcgca
     4381 gtatttggcg tcggcacaaa ataaatgcga aatatccatc gctttccacg catatactaa
     4441 aaaataaata tggaagcgaa taaaatatcg agtggcgcaa acctgctttc agaagcagag
     4501 gaaatgctgg ttgcaccggc aaagaacgtt aaaatacgcc ttcttattat ctttaacttt
     4561 atcgtcagtt tgcccgtgtt ggtgtttaac actttgacgc ttgtgaagtt tggtaatttt
     4621 gccaaccctg cgcgggactg gtatttatac cccacgttgc atttaattat gctctcgtcg
     4681 ggggtctata atttaagcca acaaataaaa ggcacgagtg gaaaccccat tgtagtgcga
     4741 ttatcgacat gggcgacggt tcttattttg caacttgttt cgattgtgac cgtgcaggcg
     4801 aatagtaatt taccgctcag ttttttgtgg gatgtctcta tctcgcttgt gcttatttat
     4861 ttgacggcgg tgatgctcaa ccgttgggcg gcgttggtgt acgctgcggt ggcgttctcg
     4921 aatatgcttt ttttggccag ccgcctcggt tttgattttg agtacgtgct ccaagcgccg
     4981 ccgggttttc cgtttcaact gccggcaaaa ggtgtattgc aattcgtcat tctttggggt
     5041 ctcttgatgt cgatttctgt cttggtggca ttttccgagg gcggcgtcat tggaaaaatt
     5101 ttgaaagcaa taccgcaagt ggtgaataaa atccgcgacg cgggagatac acaacgtcat
     5161 cttgaaatgg caaatatgcg gatgggcg
//
LOCUS       DAOMPD010000164         5180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6734_length_5180_cov_3.010146, whole genome shotgun sequence.
ACCESSION   DAOMPD010000164 DAOMPD010000000
VERSION     DAOMPD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6734_length_5180_cov_3.010146"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /gene="rpoB"
                     /locus_tag="PLY93_06875"
     CDS             complement(<1..2235)
                     /gene="rpoB"
                     /locus_tag="PLY93_06875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRP69237.1"
                     /translation="MADSNVVNVGENTSQFKIVNFAEIRKSLDVPPLIEVQTISYDQF
                     LQVGTPMAKRWRTGLQSVFEDSFPIESSNGDVVLEFVEYTLGEPKRDIWDCKVNGFTY
                     AAPLKATIRLIAIETGEVREQEVYMGDLPMMTPTGTFVINGAERVIVNQLHRSPGIFI
                     FFDEAKNVFSSRIIPDHKGSWLEFEMDAKGLLIARIDRRKKFPFTLLLKALGHGTDEQ
                     ILRLFYESESVSIKGVQAKTLAKFINRRTLTDIVHPETGEILIEAGHALNEDNLDILK
                     DAKIDKIELLVNKGGVDHRVVLNSLDKDGVNSTEEALIEFIKNQKPLDYSPDHGDEEK
                     RAKNLERANAELQRLFFDPKTYNMGSVGRYKINSRFKHLNPSEFGDDVDTQTLRPIDI
                     VETCKFMVNLIHEVPGYQYDDIDHLGNRRVRTVGELLTTQLKAGFSRMERVVKERMSM
                     NDVDIMTPQLLISIKPITAVMNEFFGASQLSQFMDQTNPLSEITHKRRLNALGPGGLT
                     RERAGFEVRDVHYTHYGRLCPIETPEGPNIGLITSMATYSRLNPYGFVETPYRIVEKG
                     KDTGKTMFLSANEEDHYVVAQANAKLGDDGEFLDKLVSCRYRGDFPLKSPEEIQLMDV
                     APTQVISLSTSLIPFLEHDDANRALMGSNMQRQAVPLLNPDMPYVGTGMELPIAYDSG
                     VCTIADYDGMVTKIDADAIEVEREDGTKDKYRLVKFKRTNQSTTLNHQALVTCHNAPE
                     SGKIT"
     gene            complement(2358..2732)
                     /gene="rplL"
                     /locus_tag="PLY93_06880"
     CDS             complement(2358..2732)
                     /gene="rplL"
                     /locus_tag="PLY93_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRP69238.1"
                     /translation="MVQELLDKIGSLTIVQAAELVKAMEDKFGISAAAPVAVAAAAPA
                     AGAAPAGEEKTEFNVVLEGFSVDKNVDAIKKVREITNLPLMDAKKLVEGGTGVLKEGV
                     SKEEAEKIKKEIEALGGKIALK"
     gene            complement(2772..3299)
                     /gene="rplJ"
                     /locus_tag="PLY93_06885"
     CDS             complement(2772..3299)
                     /gene="rplJ"
                     /locus_tag="PLY93_06885"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRP69239.1"
                     /translation="MPSVQKTTLRDEYAGILKDYPNFIVTQYQGLDVAALTALRKKLR
                     EAGGKYAVVKNNVFALALKDSGVAKDFAYDSTLKGPNAIAFVKGDVPAVAKVLRDFSK
                     ENDKLVLTAGVMESAFFDANGVKAIADLPSREQILAQIAAMLNAPATKIAGTLNNIVA
                     SLARGIKSVAEKNAK"
     gene            complement(3299..3982)
                     /gene="rplA"
                     /locus_tag="PLY93_06890"
     CDS             complement(3299..3982)
                     /gene="rplA"
                     /locus_tag="PLY93_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRP69240.1"
                     /translation="MAKHGKKYRAAADKIDVDKEYSLLDAVKLLKEVSYTKFDSTLEG
                     HFNVKYKSSQNVRGIVQLPHGTGKKVKILVFAKGEKAEEARQAGADYVGDDDLIQKIQ
                     GGWIDFDFVVATPDMMKDVGKLGQVLGKRGLMPKPKSGTVTTDMKGIITQLTSGRIEY
                     RADKTGVVHVPMGRLSFNPEQMKENAATVFQAILKDKPADAKGDYVVSMFVTGTMTPA
                     IRVNIKEMR"
     gene            complement(3985..4416)
                     /gene="rplK"
                     /locus_tag="PLY93_06895"
     CDS             complement(3985..4416)
                     /gene="rplK"
                     /locus_tag="PLY93_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRP69241.1"
                     /translation="MAAKKIIAQVKLQIPAGKANPAPPVGTALGPQGVNLMEFCTQFN
                     AKTQAQAGSIIPVVVTVFSDRSFTFITKVPPASDLIKKAAKIESGSKIPNKEKVGSLS
                     DAQLEAIAKQKIPDLNANDIAAAKLIIAGTARSMGVTIQGG"
     gene            complement(4474..5010)
                     /gene="nusG"
                     /locus_tag="PLY93_06900"
     CDS             complement(4474..5010)
                     /gene="nusG"
                     /locus_tag="PLY93_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRP69242.1"
                     /translation="MAMHWYVLTTLSSHEDKVRKLIERQKEKWPDGAKVGQVKIPLHE
                     MAELRGGKKRVVKKKLMPGYVFLEAELTDELHQKIRALPGIMGFVSAGTEPQILTEEE
                     MHSLFTEMGAAVQDEKQAPRIFFSECEIVRIIDGPFANFQGTVDDVNPEKGKVRVRVE
                     IFGRATPVELDYLQVSKI"
     gene            complement(5012..>5180)
                     /gene="secE"
                     /locus_tag="PLY93_06905"
     CDS             complement(5012..>5180)
                     /gene="secE"
                     /locus_tag="PLY93_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRP69243.1"
                     /translation="FFVEARAELRKAVWPSWDEVSRSTIVVFVTVILFAVLLYLVDDG
                     IDRVVTLLLGG"
BASE COUNT         1246 a         1299 c         1166 g         1469 t
ORIGIN      
        1 tgtaatttta ccgctttcag gcgcattgtg gcaagtcacg agcgcctggt ggtttagggt
       61 tgtgctttgg ttggtgcgct tgaatttgac taaacggtat ttatcttttg taccgtcctc
      121 gcgctcgacc tcgatagcgt cagcgtcgat tttagtaacc atgccatcgt agtcggcaat
      181 cgtacacacg ccggaatcgt acgcaatggg gagctccatc cctgtaccga cgtacggcat
      241 gtcggggtta aggagcggca ccgcttggcg ttgcatgttt gaacccatga gcgcgcggtt
      301 ggcgtcatcg tgttccaaga aaggaattaa actcgtggaa agtgaaatca cttgggtagg
      361 agcaacgtcc atcaactgaa tttcttcggg tgatttaagt gggaaatccc ctctataacg
      421 gcagctcacc agtttatcta aaaactcacc atcgtcaccg agttttgcgt tcgcctgagc
      481 gactacgtag tggtcttctt cgtttgcgga caagaacatc gttttaccag tatctttgcc
      541 tttttcaaca atccggtagg gtgtttcgac aaagccatac gggttgaggc gtgagtacgt
      601 cgccatcgag gtgataagac cgatgtttgg accttctgga gtctcaatgg ggcaaaggcg
      661 gccataatgt gtgtagtgca cgtcacgcac ttcaaaaccg gcacgctcgc gcgttaatcc
      721 acccgggccc aacgcgttca agcggcgctt atgcgtaatt tcagaaagcg gattggtttg
      781 atccatgaac tgtgaaagtt gactcgcgcc aaaaaactca ttcatcaccg ccgtaatggg
      841 tttaatagaa attaaaagct gcggggtcat gatgtccacg tcgttcatgc tcatccgttc
      901 tttcactaca cgttccatgc gcgagaagcc cgctttcagt tgcgtcgtga ggagttcgcc
      961 cacagtacgc acccggcggt tacccaagtg atcaatatcg tcgtattgat acccaggcac
     1021 ttcgtggata agattcacca tgaacttaca ggtttctaca atatcaatgg ggcgtagcgt
     1081 ttgtgtatcg acatcatcgc caaactcact gggattcaag tgcttaaagc gcgaattaat
     1141 tttgtaacgc ccaacgctac ccatgttgta cgttttagga tcgaaaaaga ggcgttgcaa
     1201 ttcggcattg gcacgctcta aattcttcgc acgcttttct tcatcgccgt ggtcgggact
     1261 atagtcgagt ggcttttggt ttttaataaa ctcgatgaga gcttcttcgg tggagttgac
     1321 gccgtcttta tcgagtgagt tgagcacgac acggtgatcg acaccaccct tgtttacaag
     1381 gagttcaatc ttatcgatct tggcgtcttt gagaatatct aaattatctt cgttgagcgc
     1441 atgccctgcc tcaatgagga tctcccctgt ttcgggatga acgatgtccg tcaacgtgcg
     1501 tctgttgata aattttgcca gcgtttttgc ttgcacgcct ttaatcgata cgctttcgga
     1561 ctcataaaac agccgtaaaa tttgttcgtc ggtgccgtgc cccagcgctt tcaaaagaag
     1621 cgtgaaaggg aattttttgc ggcggtctat acgcgcaatg agaagacctt tcgcgtccat
     1681 ttcaaattcc aaccacgagc ctttgtggtc ggggataata cgcgatgaga acacattctt
     1741 cgcttcgtca aaaaagataa aaatcccggg agaacggtgt aactggttca caatcacgcg
     1801 ctcggcgcca ttgatgacga acgtaccggt gggggtcatc atgggtaaat cgcccatata
     1861 cacttcttgt tcgcgcacct cacccgtttc aattgcaatg agtcgaatcg ttgccttgag
     1921 cggcgctgcg taagtaaagc cgttcacttt acaatcccat atatcgcgtt tgggttcgcc
     1981 caacgtgtat tcaacaaatt caagcaccac gtcgccgttt gaactttcga tagggaaact
     2041 atcttcaaac accgactgta accctgtgcg ccagcgtttt gccatcggcg tacccacttg
     2101 caaaaactga tcgtatgaaa tcgtttgaac ttcgataagt ggtggaacat ccaacgattt
     2161 acgaatttca gcaaagttta caattttgaa ttgtgatgta ttttcaccca cattgacaac
     2221 attagaatcg gccattgttc tcctcaatac ccgtacactg aaaaaaagaa aaaatacggg
     2281 ggataaaata acaatcccac cgggatttac cccggcaggt tatataaaaa gaattgacta
     2341 gataaaaaag aaacgcatta cttgagcgca atcttgccgc ccaaggcttc aatttctttt
     2401 ttaatttttt cagcttcttc ttttgaaacg ccttctttca gaacgcctgt accgccctca
     2461 acgagcttct ttgcgtccat caaaggtaaa ttcgtaatct cacgaacttt tttaatggcg
     2521 tctacgtttt tatcgacgct aaagccttcg agaactacgt taaattccgt tttttcttca
     2581 ccggcgggag ctgcgcccgc tgcaggtgct gccgctgcga ccgccactgg ggcagctgca
     2641 gaaatgccga atttgtcttc cattgccttt acgagctcgg ccgcttgcac gattgtgagc
     2701 gaaccaattt tgtctaagag ttcctgtacc atactttctc ctttaatctt cccgcttata
     2761 taattaagcc attattttgc gtttttctct gcaacgcttt ttatgccacg cgcgagcgag
     2821 gccacgatat tgttgagtgt gccagcaatt ttagtcgcag gcgcgttgag catcgctgca
     2881 atctgtgcca gaatttgctc ccttgaaggt aggtcggcaa tcgctttgac gccgttcgca
     2941 tcaaagaaag cgctttccat aacacccgca gtgagcacca gcttgtcgtt ttcttttgag
     3001 aaatcacgta acacttttgc cacagcgggc acatcgcctt taacaaaagc gatcgcatta
     3061 ggacctttga gcgttgaatc gtaagcaaaa tcttttgcta cacccgagtc cttgagcgca
     3121 agggcgaaca cgttattttt tacaactgcg tatttaccgc ccgcttcacg cagtttttta
     3181 cgtaaagcag tgagcgccgc cacatccaaa ccttggtatt gcgtgacgat aaaattggga
     3241 taatctttta atatacctgc gtactcatcg cgcagtgttg ttttttgtac tgacggcatt
     3301 agcgcatttc cttaatgttt actcgaatcg caggggtcat ggtgccggtg acaaacatcg
     3361 aaaccacgta atcgcctttt gcgtcggcgg gtttatcttt gagtattgcc tgaaaaacag
     3421 tggctgcatt ttctttcatt tgttctggat tgaacgaaag gcgtcccatg gggacatgta
     3481 ccacgcctgt tttatcggca cgatactcga tacgtccaga ggtgagctgt gtaataattc
     3541 ccttcatatc ggttgtcact gtgcccgatt tgggttttgg catgaggccg cgcttaccca
     3601 aaacttgccc caacttaccc acgtctttca tcatgtcggg ggtcgcaacg acaaagtcga
     3661 agtcgatcca ccccccttgg attttttgga tcaagtcgtc gtcgcccaca tagtcggcgc
     3721 cggcttggcg tgcttcttcg gctttttcgc ctttggcaaa aacaagaatc tttacttttt
     3781 taccagtgcc gtgaggcaac tgcacgatac cacgcacatt ttgcgaactc ttatacttta
     3841 cgttaaaatg cccttcgagc gtggaatcaa actttgtata tgaaacttct ttaaggagct
     3901 tcactgcatc gagcaatgag tattctttgt cgacatcaat cttatctgcc gcagcgcggt
     3961 atttttttcc gtgttttgcc attgttagcc gccttgaatt gttacaccca tggaacgcgc
     4021 tgtacctgcg ataatgagct ttgccgctgc aatatcgttt gcgttcaaat cggggatttt
     4081 ctgctttgca attgcttcca actgcgcatc tgaaagcgag cccacttttt ctttgttagg
     4141 aatttttgaa ccgctttcaa tttttgccgc tttcttaatg agatcactcg caggaggaac
     4201 ttttgtaata aaagtaaaag aacgatcgga aaacacagta acaacaacag gaatgatgct
     4261 acctgcctgc gcctgtgttt ttgcattaaa ctgcgtacaa aactccatga gattgacgcc
     4321 ttgcggcccc aacgctgtac caactggggg tgcagggttt gccttaccgg cgggaatttg
     4381 tagctttact tgagcgatta ttttttttgc tgccatgaac gaaaccttat ctattgcgag
     4441 ctgccatttt tataatccgt gacatgccgc aaactatatt ttacttactt ggagataatc
     4501 caattcaacg ggagttgcac gcccaaaaat ctcgacgcgc acccgaacct ttcctttttc
     4561 agggttcaca tcgtcgaccg taccttgaaa gttggcgaaa ggaccgtcaa taattctgac
     4621 aatctcgcac tcggagaaga aaattcgcgg cgcttgtttc tcgtcctgca ccgccgcccc
     4681 catttcggta aaaagcgaat gcatctcttc ctcggtgagt atttgcggct cggtgcctgc
     4741 gctcacaaag cccataatgc ccggcaatgc acggattttc tggtgtaact catcggtgag
     4801 ctccgcctct aaaaaaacgt acccgggcat gagcttcttt tttacaacgc gctttttacc
     4861 gccgcgcaat tcagccatct cgtgtagagg gattttcacc tgccctactt tggccccgtc
     4921 tggccatttt tctttttgac gctcaatcag tttacgtact ttatcttcgt gcgacgaaag
     4981 tgtcgtcagc acataccagt gcatcgccat atcagccccc taaaagaagc gtcacaacgc
     5041 gatcgatacc gtcgtcgacc agatataaga gcacggcaaa aaggataacc gttacaaata
     5101 caacaatggt cgagcgagaa acttcatccc acgaaggcca caccgctttg cgtaactctg
     5161 cgcgcgcttc gacgaaaaat
//
LOCUS       DAOMPD010000165         5146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6810_length_5146_cov_2.972108, whole genome shotgun sequence.
ACCESSION   DAOMPD010000165 DAOMPD010000000
VERSION     DAOMPD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_5146_cov_2.972108"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLY93_06910"
     CDS             <1..566
                     /locus_tag="PLY93_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69244.1"
                     /translation="LPFINAVGTFKERLNVGAALTSTVLYALAFFIYLLVTKALLSSL
                     KSDTALIALLVVLLLIIFVPLRLAQKAFFREELNRAGVHFWILFFLTIALFVNPIVAL
                     FGGVFLMLLSAFHRVGLLSKTLRIVSIVLLVFWVVLIATKPFGSFANYLSALLPHSSA
                     VGVLKLSLAAFVGGGLVSLVFVPRERN"
     gene            520..1524
                     /locus_tag="PLY93_06915"
     CDS             520..1524
                     /locus_tag="PLY93_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRP69245.1"
                     /translation="MAVSSRSYLSPVSAINAAVIGCGRIAWMLETDPLRYKPCTHLGA
                     LVRWQKKRALQVTALCDSDFIRAKDAAHFFHLKNSFLTNNYKEIILQKPNLLVIAAST
                     AAHSTILSAALDARIERIVVEKPVAFSAKEARLLRKKITKSKSIVLANYERRYHPKYI
                     ALQKETEDAISYRGVFAASGHMLYPHKKSGDEGVLLHDTTHLLDLAQFYFGRVCRYKK
                     IAEKNRHVLYLEHASGVHGVIETALNVGVFHLELELHTKQKRIRVGNGFLTMQEIQTS
                     PHYKNFKSYAKDIYKEDKPFSVAQNPFIKLYETLLEGRATNDSIFDALQNVEILGEMI
                     "
     gene            complement(1507..1923)
                     /locus_tag="PLY93_06920"
     CDS             complement(1507..1923)
                     /locus_tag="PLY93_06920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRP69246.1"
                     /translation="MSKVSFTLDAQLAADTLAVTDLSLCTVRLMNTTAVPWLVLAPQR
                     PDKKEIIDLNISEQAQLWKEITAISHVLKDVFNPTKLNVAALGNAQLHIHVIARFAND
                     AAWPQPVWGKVPMEAYLPEVAQERINKITHALNHLS"
     gene            complement(1916..2569)
                     /locus_tag="PLY93_06925"
     CDS             complement(1916..2569)
                     /locus_tag="PLY93_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRP69247.1"
                     /translation="MKYRHFALDIDGTVFSSEEIIYPVYKEAIENYCKTHNATLVVPP
                     RERIMLEIGKPVKKIFENLFPALTEGERDKISDSILGLLAAKIKNGGGEYYPDVAHTI
                     ETIHQKGGIILVASNGRQPYIDAILDYIGVLQYVTMPTYIDGDKIVTKSDILKTYVDA
                     GFPAKEILMVGDRLSDYDAARAIDCDFAWCAYGHAPQGEIADYTVRLEKFTDLIQYV"
     gene            complement(2574..3182)
                     /locus_tag="PLY93_06930"
     CDS             complement(2574..3182)
                     /locus_tag="PLY93_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69248.1"
                     /translation="MKSRYVLFLLLLVVVGIFLVLFLQPCSKTPPVAGIVPAAEEVSR
                     EKKMDTPSALTRPPTQKIQETTEKSDKPKAGITSPVPAKVAPRAEAIDTSEPVHLSSS
                     DTLPDISKCVSANFPDAAKPYVQNAVVTVRLTVDRFGRVRENKPIKVDLPPDLEYEQT
                     ASMRKLFIQAGTRAFGSKKCPPHIVNGQNSGYVIEVPLQYKQ"
     gene            complement(3206..3844)
                     /locus_tag="PLY93_06935"
     CDS             complement(3206..3844)
                     /locus_tag="PLY93_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69249.1"
                     /translation="MTNSFRRERDKKKRRYLAITLSLALLIEATVYFFSVWRDESHEI
                     AFSSISVDLGDFKPRARRAPVVEIDEVFGNQFVKKEEQTQLPANEAGGSIDGEGVVDL
                     DVEGTGTGGGAPNIARCALRNFPAEAKSYVEEALVPIQITVDRTGLVREAKPLFVQIN
                     KELPAELKTRMKALFMKAARDSLLGRKCPLYYVNGEPRPYKLEVPLSYELYN"
     gene            complement(3900..4376)
                     /locus_tag="PLY93_06940"
     CDS             complement(3900..4376)
                     /locus_tag="PLY93_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRP69250.1"
                     /translation="MDNAKENITREMMLLKERMHQRQYKSTPQRDKIAEWIFRTHEHF
                     TVEELITSFREQGKKVSQATAYRVIQMMLELRLIEEHDFGKDYKFYEHTPGHQHHDHI
                     VCIDCGKIIEFSDSVLENLKEKITLKNGFRMKKHYLTIYAECTTCPPEKKILTNKK"
     gene            4520..>5146
                     /locus_tag="PLY93_06945"
     CDS             4520..>5146
                     /locus_tag="PLY93_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRP69251.1"
                     /translation="MRWTFLFMLLGVLVVTNCRSAPVKTDATEAQGDAFDADLPVNDP
                     LEGMNRGVFKFNDIFFRYLVNPIATTWNFITPRFLRTGIDNFFNWIYTPGRLVNNLLQ
                     AKFVGAAKEIGYFVINGTVGLLGFYDASADIFELQKTNEDTDQTLGKWGIAEGAFINY
                     PFIGPKTVRGTFGFAGDLLLQPQTVFIPRYVKPQTMWQQIGIVVGTYTV"
BASE COUNT         1274 a         1194 c         1193 g         1485 t
ORIGIN      
        1 tactgccctt tatcaacgca gtgggcacat tcaaagaaag attaaacgtg ggggccgcac
       61 tcacatcgac cgtgttatat gcattggctt tctttattta tttgctcgtc acaaaagcac
      121 tcctctcttc tttgaaatcg gataccgcac tcattgcgct cttggtggta ctcttactca
      181 taatttttgt gccattgcgt cttgcacaaa aagcattttt ccgcgaagaa ctcaaccgtg
      241 caggcgttca tttttggatt ttattttttt taacgattgc acttttcgtt aacccaattg
      301 tggcattatt cgggggagtg tttttaatgc tactctcggc gtttcatcga gtaggccttt
      361 tgagtaaaac gctgcgcatt gtttctattg tgctgttggt attttgggtt gtccttattg
      421 ccactaaacc gttcggtagt tttgcaaatt atttatccgc actgttgccc cattcttcag
      481 cggttggagt cttgaaactc tctctagcgg cgttcgtcgg tggcggtctc gtctcgctcg
      541 tatttgtccc ccgtgagcgc aattaacgca gcagtcattg ggtgtgggcg catcgcgtgg
      601 atgcttgaaa ccgacccctt acgctataaa ccgtgcacgc acttaggcgc gctggtgcgt
      661 tggcaaaaaa agcgcgcgct gcaagtcacc gcgctttgcg acagcgattt tatacgggca
      721 aaagacgccg cccatttttt tcatctgaaa aactcttttc taacaaataa ctataaagaa
      781 attattttac aaaagcccaa cttgctggtg attgccgctt caaccgctgc gcactctacc
      841 attttaagcg ctgcgctcga cgcccgcatt gaacgaattg tcgttgaaaa accggtggcg
      901 tttagcgcaa aagaagcgcg gcttttgcgc aaaaaaatta caaaaagtaa atccatcgtt
      961 ttggctaatt acgaacggcg ctaccacccc aaatacattg cgttacaaaa agaaaccgag
     1021 gatgctattt cttatcgagg cgtttttgcg gcgagtggtc acatgctata cccacataaa
     1081 aaatcgggcg acgagggtgt gcttctacac gacacaacgc atcttttaga tttagcccag
     1141 ttttattttg gccgcgtatg ccgttataaa aaaattgctg aaaaaaatcg acacgtgctc
     1201 tacctcgaac atgcatcagg cgtacacggc gtgatcgaaa ccgcgctcaa cgtgggtgta
     1261 tttcatcttg agttggaatt acacacaaaa caaaagcgta ttcgtgtcgg taacggtttt
     1321 ttaacgatgc aagaaatcca aacaagccct cattacaaaa acttcaaatc gtatgcgaaa
     1381 gacatttata aagaagacaa accattctcg gtggcgcaga acccatttat aaaactctat
     1441 gaaaccctac tcgaagggcg cgccaccaac gatagcattt ttgacgcgtt acaaaatgtc
     1501 gaaattttag gagagatgat ttaacgcatg cgtaatttta ttaatccgct cttgcgccac
     1561 ttcgggtaaa tacgcctcca tcggcacttt gccccagacc ggttgaggcc acgccgcatc
     1621 gtttgcaaac cgcgcaatca catggatgtg cagttgtgca ttgcccagtg cggccacgtt
     1681 taactttgtg ggattaaaca cgtctttcaa aacatggctt atggcggtga tttctttcca
     1741 aagctgtgct tgttcactta tgttcaaatc gatgatttct tttttgtcgg ggcgttgtgg
     1801 cgctaaaact aaccaaggca ccgctgtggt gttcataagg cgcacagtgc atagggataa
     1861 atcagtaacg gcaagcgtat cggcagcaag ctgtgcatcc aacgtgaacg atactttaga
     1921 catactggat tagatcggta aatttttcta agcgtacggt gtagtcagcg atttcacctt
     1981 ggggggcgtg gccgtatgcg caccacgcga aatcgcaatc gatagcacgc gcagcgtcgt
     2041 agtcgctcaa gcggtcgccc accatgagta tttcttttgc aggaaacccc gcatccacat
     2101 acgtcttgag aatatcgctt ttggtaacaa ttttatcgcc gtcgatatat gtcggcatgg
     2161 tgacgtattg caaaacaccg atataatcta aaattgcatc gatgtaaggt tgccgtccgt
     2221 tggaggcgac gagaataatt cctccttttt gatggatggt ttcgatggta tgcgcaacgt
     2281 cgggatagta ttcccctccg ccatttttaa tttttgccgc aaggagcccc aggatgctgt
     2341 cggatatttt gtcgcgctcg ccttcggtta aagcgggaaa taagttttca aaaattttct
     2401 taaccggttt gccaatttca agcatgatgc gttcacgcgg cggtacaacc agcgtggcgt
     2461 tgtgggtttt gcaataattc tcaatcgctt ctttataaac ggggtagata atctcctcgg
     2521 aagagaaaac agtgccgtcg atgtcgagcg cgaagtggcg gtacttcatg tttttattgt
     2581 ttatattgca gcggcacttc aatcacatag cctgaatttt gaccatttac aatatgcggt
     2641 gggcattttt ttgaaccaaa cgcccgcgtc cccgcttgga taaaaagttt gcgcattgag
     2701 gccgtttgtt catattctaa atcgggagga agatccactt tgatgggttt attttcacgc
     2761 acccgaccaa aacgatcgac ggtaagacgt acggtgacca ctgcattttg cacatacggt
     2821 ttggccgcat cggggaaatt ggcggagaca catttactga tgtcgggcaa ggtatcgctg
     2881 ctggaaaggt gtaccggctc ggaggtatcg attgcttcag cgcgtggcgc cacttttgcg
     2941 gggacaggac tagttatccc cgctttgggt ttgtctgatt tttccgttgt ctcttgaatt
     3001 ttctgcgttg ggggacgagt aagcgcagaa ggagtatcca tttttttttc gcgtgagacc
     3061 tcttctgctg ccggtactat accagcgaca gggggagtct tactacacgg ctgtaaaaac
     3121 aagacaagga aaatccccac tacaaccagc aagagtaaaa aaagtacata ccttgatttc
     3181 atagaagaag tttttcgata cgccattagt tgtaaagctc ataactaaga ggcacttcga
     3241 gcttgtacgg gcgcggttca ccgttcacat agtagagcgg gcatttgcgc cccaaaagcg
     3301 aatcgcgcgc cgctttcata aaaagcgctt tcatgcgtgt tttgagttca gctggtaact
     3361 ctttgttaat ttgaacaaaa agcggcttgg cttcgcgcac gagccctgtt ctgtccacgg
     3421 tgatttgaat tggcaccaac gcttcctcga catacgattt tgcctcggcg ggaaaattgc
     3481 gtagcgcaca gcgcgcgatg ttcggcgcgc ccccacctgt gcccgtgccc tcaacgtcta
     3541 agtcaaccac gccttcgccg tcgatactcc cacccgcttc gtttgcaggc agttgtgttt
     3601 gttcttcttt tttgacaaat tggttgccaa acacttcgtc gatttcaact acaggtgcgc
     3661 ggcgggcgcg gggtttaaaa tctcccaagt cgactgaaat cgacgagaat gcaatttcat
     3721 ggctttcgtc gcgccacaca gaaaaaaaat aaaccgtcgc ttcaatgagt aacgcgagcg
     3781 ataacgtaat tgcgaggtat cggcgttttt tcttatcgcg ctcgcgacga aaagagttgg
     3841 tcactccgtc gaaaatgcga acgccattcg aaagtagaat gtcatccatt gctttttatt
     3901 tacttcttat ttgtgagaat ctttttctca ggcgggcaag ttgtacattc ggcataaatc
     3961 gtcaaataat gcttcttcat acgaaaacca tttttaagag taattttttc tttgaggttt
     4021 tcgagaacgg aatctgaaaa ttcgataatt ttgccacaat cgatacacac aatgtggtcg
     4081 tggtgttgat ggcctggggt atgctcgtaa aatttatagt ctttaccaaa atcgtgctct
     4141 tcgatgaggc gcaattcaag catcatttga atgacacgat acgcagtcgc ttgcgagact
     4201 tttttccctt gttcgcggaa cgatgtgatt aactcttcca cggtaaaatg ctcgtgcgta
     4261 cggaaaatcc actcggcaat tttatcgcgt tgtggggtcg atttatattg ccgctgatgc
     4321 atgcgttctt tgagcagcat catttcgcga gttatatttt cttttgcatt atccataaac
     4381 acaattaaag aggactatcc gtaatataca gtaaaatata gcgctttgtc tatatttcgt
     4441 gcttgttaga gcctgtgcgg caactcacaa attaaaaatc gtttacgcgg ctcatttctt
     4501 ttagagtgtt acggacatcg tgcgttggac ttttttattc atgcttttag gtgttcttgt
     4561 ggtgacgaat tgccgcagcg caccggtaaa aaccgacgcc actgaagccc aaggcgatgc
     4621 gtttgacgcc gatttacctg tcaacgaccc gctagaagga atgaatcgag gggtatttaa
     4681 gtttaacgat atttttttcc ggtacttggt gaatcctatt gccaccacat ggaattttat
     4741 tactccgcgt tttttgcgca ctggcatcga caattttttt aattggattt atacccctgg
     4801 ccgcctcgtg aataatcttc tccaagcaaa gtttgtcggt gccgccaaag aaatcggtta
     4861 ctttgtgatt aacggtacgg tgggtctttt gggtttttac gacgcctccg ccgatatttt
     4921 tgaattgcaa aagaccaacg aagacaccga ccaaaccctc ggcaagtggg gcatcgccga
     4981 aggggctttc attaactacc cgttcatcgg ccccaaaaca gtgcgcggca cgtttggttt
     5041 tgcgggtgac ttgctgttgc aaccgcaaac ggtgtttatc ccccgttatg tgaagccgca
     5101 aacaatgtgg caacaaatcg gcattgttgt gggtacgtac accgtc
//
LOCUS       DAOMPD010000166         5130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6841_length_5130_cov_2.280000, whole genome shotgun sequence.
ACCESSION   DAOMPD010000166 DAOMPD010000000
VERSION     DAOMPD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6841_length_5130_cov_2.280000"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="ruvB"
                     /locus_tag="PLY93_06950"
     CDS             complement(<1..950)
                     /gene="ruvB"
                     /locus_tag="PLY93_06950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRP69252.1"
                     /translation="MIEPHLQLEKNEQENQVRPTLLDDFIGQKDLVENLRSFIKAASI
                     RREPLDHILLSGPPGLGKTTLARIVAQETGTRFSQVTAPNIKRPGDLAKILSGLGEGD
                     VLFIDEIHRLPVAAEELLYGAMEDRKIDIAVGDAALANSVQITLPPFTLVGATTRPGD
                     LTAPLRDRFGIKFHLEFYDDDDLVRILERAAKLWKIPAQAEATLAIACRSRSTPRIAL
                     HLLRRVWDYALAESESLTHAVSLDAALHAFAKMHIDTEGMTKLDRQFLLTIAKHYQGG
                     PVGLKPLSSILAEDLATLEDYVEPYLVRRAFIRRTPRGRML"
     gene            complement(947..1336)
                     /gene="acpS"
                     /locus_tag="PLY93_06955"
     CDS             complement(947..1336)
                     /gene="acpS"
                     /locus_tag="PLY93_06955"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRP69253.1"
                     /translation="MIVGVGIDICENDRIAEMLEKYKNRFLERVFTKDEITYCLNKKE
                     PTPHLAARFAVKEAFIKALGMPRDLRISYRDVGLKGSEGKKDIHVTGLLAEMLASKSV
                     TNILFSISHARDYSNACVILERDTTAA"
     gene            complement(1336..3987)
                     /gene="alaS"
                     /locus_tag="PLY93_06960"
     CDS             complement(1336..3987)
                     /gene="alaS"
                     /locus_tag="PLY93_06960"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRP69254.1"
                     /translation="MKINEIRNSFLSFFESKKHLRVPSASLLPAADPTLLFTTAGMVP
                     FKDYFSAAKVPPSPRLVSVQKCLRTTDLESVGKTKRHLSFFEMLGNFSFGDYFKKEAI
                     EFAWEFSTQGLRFPKEKIWISVFENDDEAVDIWHKHVGVPREKIVRLGRADNFWGPAG
                     EAGACGPCSELYLDRGEEFGTDKENQAPGGSGDRFMEFWNLVFNQFDFQNGAYLPLKQ
                     TGIDTGAGLERLATLTQGVDSVFDTDELMRLRMKAAEVFNVEYSGSSVVAVRVLTDHI
                     RTLTFAMSDGIFPSNESRGYVLRRVLRRAMLFGRKLGQKEALLHRLSDIYGDFYAELK
                     GNIPFIRDYIHEEENRFLKTLDSGAHRLDEMLAVAKEAKAAMLSGKEVFQLYDTFGFP
                     LEMTVELAEQDGLRVDIDAFKAEMEKQRERGRAAWKGASTLPLPPDTHTEFTGYASLV
                     GQGKITALIRDNESVDALGENDNGALCFLVSDKTPFYAEGGGQLGDIGFIKTTSGTVR
                     VTDCRKQGDAYIHLLSELEGSLRVGESAGFSVEKENREALMRHHSATHLLNAALRNAL
                     GNHIKQTGSLVHPDYLRFDFSHPKALAANELVAVEQAVNDAVAANAKVETLVLTKSEA
                     EKRGAVMTFGEKYGDVVRVVEMGTFSKEFCGGTHVQSTGGIGAFFILKETSPGAGNRR
                     IEAVAGLQAQKVFADKLQQLKELSAEINDASLKKKLDTLEKESREGATPPLWRKALEL
                     LAESTAAVEALRKEKKKSKDTSDTVLPFVDELVQAANETKTGISFAAKSFPVQNINDL
                     KTLVDKAREKKARAVYLVFSSSENGTVFVCGTSAEYAAEKKIHLGQLVKKAPSTLGLT
                     GGGKAEFVQGKLTGSAGAVQEFFTAEF"
     gene            complement(4101..4610)
                     /gene="rplQ"
                     /locus_tag="PLY93_06965"
     CDS             complement(4101..4610)
                     /gene="rplQ"
                     /locus_tag="PLY93_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRP69255.1"
                     /translation="MNKGNKVKHLNKKVGHRQAMLGNLVQSLLYHENITTTVAKAKVT
                     RQIAEKLITRARENQNSALSAAEKVHNLREAAKIVKDKEILHKLFDDIAPRFVSRPGG
                     YTRILRVGQRVSDASEMAIIQLVERKELAQLKDDRKAVRAARLPKKKRAVSATKSDKS
                     AKEKKATKK"
     gene            complement(4640..>5130)
                     /locus_tag="PLY93_06970"
     CDS             complement(4640..>5130)
                     /locus_tag="PLY93_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRP69256.1"
                     /translation="KKMIDEVGVIPIDALFSPITKVNFDITETRVDQRTDYDKVTFEI
                     WTDMTISPEDAMAYAAKILKEHLTIFINFAEEMYEEEEEFDETDERLKKAMQIALEDM
                     EMSVRTIAVLRSLDMRNVKDIVTRYEDDLRKSKHYSDKVLLQLKSKLANMNLAFGMRD
                     GR"
BASE COUNT         1209 a         1312 c         1168 g         1441 t
ORIGIN      
        1 agcatgcgcc cgcgtggtgt tcggcgtata aaagcgcggc ggactaaata tggttcgaca
       61 taatcttcga gcgttgctaa gtcctcggcc agaatcgacg acaacggttt taatcccacc
      121 gggccacctt ggtaatgttt tgcaatcgta agtaaaaatt ggcgatcgag cttggtcatc
      181 ccctcggtgt cgatgtgcat ttttgcaaat gcgtgaagcg cagcatcgag cgacaccgcg
      241 tgcgtgagcg actcgctttc agcaagggcg taatcccaca cgcgccgcaa aagatgcagt
      301 gcgatacgcg gcgtgctacg cgaacggcaa gcaatcgcca gcgtcgcttc ggcttgcgcc
      361 ggtattttcc aaagcttcgc cgcgcgttct aagatacgca ccaagtcgtc atcgtcgtaa
      421 aattctaaat gaaatttaat accaaaacga tcgcgcaaag gtgcagtcaa gtctcccggc
      481 cgcgttgtcg ctccaacaag agtaaaggga ggcaatgtaa tttgtactga attggcgagc
      541 gccgcatctc ccacagcgat atcaatctta cgatcttcca tcgcaccgta gagcagctct
      601 tctgcggcaa cggggagacg gtgtatctcg tcgatgaaaa ggacatctcc ctcccccaag
      661 ccactcaaaa tttttgctaa atccccaggg cgtttgatat tgggtgcggt cacttgtgaa
      721 aaacgcgtgc cggtttcttg cgcaacgata cgcgcgagcg tggttttgcc gagtcccggt
      781 gggcctgaaa gtaaaatatg gtctaatggc tcacggcgga tcgacgccgc cttaataaac
      841 gagcgtaagt tttcgactaa atctttttga ccgataaaat cgtcgagcag tgttggccga
      901 acttgatttt cttgttcgtt tttttcgagt tgtaaatgcg gttcaatcat gctgctgttg
      961 tgtcgcgctc taaaatcacg cacgcgttgg aataatcgcg cgcgtgcgaa atactaaaaa
     1021 gtatattcgt tacacttttg ctcgctaaca tttctgcaag aagaccggtc acatgaatat
     1081 cttttttacc ttcgctacct ttcaagccca cgtcgcggta agaaatacgc aagtcgcgcg
     1141 gcatacccag cgctttaatg aacgcttctt taacggcgaa gcgcgcggct aaatgtgggg
     1201 ttggctcttt tttattgaga caatacgtta tctcgtcttt ggtaaaaacg cgttccaaaa
     1261 atcgattttt atatttctcc agcatttctg caatgcgatc gttttcgcag atgtcaatgc
     1321 caacgccaac gatcattaaa actccgctgt gaaaaattct tgcaccgctc ctgcagaacc
     1381 ggtgagttta ccttgcacaa attccgcctt accgccgcca gtcagtccca acgtggacgg
     1441 cgcttttttc acgagttgtc ctaagtgaat ttttttctct gcggcgtatt ctgcgctcgt
     1501 accgcaaaca aagactgtgc cgttttcact cgatgagaag accaagtaaa cggcgcgcgc
     1561 ttttttttcg cgtgctttat ccacgagtgt ctttaagtcg ttgatatttt gtacgggaaa
     1621 actttttgcc gcaaaagaaa ttcccgtctt tgtctcattt gccgcttgaa cgagttcatc
     1681 cacgaacggc agcacggtgt ccgatgtatc ttttgatttc tttttttctt tacgcaacgc
     1741 ttctaccgct gcggtgcttt ccgctaaaag ttcgagtgct ttgcgccaga gtggcggagt
     1801 agcaccttcg cgactttcct tttcgagtgt atctaatttt ttcttcaacg atgcgtcgtt
     1861 gatttccgcg cttaattctt tcagttgttg tagtttatcc gcaaacactt tttgggcttg
     1921 gagccccgca accgcttcga tgcggcggtt accagcgcca gggcttgttt cttttaaaat
     1981 gaaaaacgcg ccaatgccgc cggtcgattg aacgtgagta ccaccacaaa attcttttga
     2041 gaaagtaccc atctctacca cacgcaccac atcgccgtat ttttcaccga acgtcatcac
     2101 cgcgccgcgt ttttccgctt cgctttttgt gagcacaaga gtttctactt tggcgttagc
     2161 agccaccgca tcgtttaccg cttgttccac ggcaacaagt tcgttcgccg ctaacgcttt
     2221 cggatgtgaa aaatcgaaac gtaaataatc gggatgcacg agcgaaccgg tttgcttaat
     2281 atggttgcca agcgcattcc tcaacgcagc gttcaaaaga tgcgtcgcgc tatggtggcg
     2341 cattaacgct tcgcggttct ctttttcaac cgagaagccc gcgctctcac cgacgcgtaa
     2401 cgagccttca agttcgctca acaggtggat atacgcgtcg ccttgcttgc ggcaatcggt
     2461 cacgcgcaca gtcccacttg tagttttaat aaaaccgata tcgccgagtt gtccgccacc
     2521 ctcggcgtaa aagggcgttt tatccgacac cagaaaacaa agcgcgccgt tgtcgttttc
     2581 accgagagca tcgacacttt cattgtctcg gatgagggcg gtgatttttc cctgtcccac
     2641 gagcgaagcg tatcctgtaa actcggtgtg cgtatcgggg gggagcggca gcgtcgacgc
     2701 gcctttccac gccgccctac cgcgctcgcg ttgtttttcc atttcggctt tgaacgcatc
     2761 gatatccacg cgcaaaccgt cttgttcggc aagttcgacc gtcatctcta aagggaaacc
     2821 aaatgtatcg tataattgaa aaacttcttt tccggaaagc atcgccgctt tcgcttcttt
     2881 agccaccgcc agcatttcat cgaggcggtg tgcccccgag tcgagtgttt tcaaaaaacg
     2941 attttcttct tcgtggatgt aatcacgaat aaaggggatg tttcctttga gctctgcata
     3001 gaagtcacca taaatatcgc taaggcgatg gaggagcgcc tctttttgtc ccagcttacg
     3061 gccaaaaagc attgcacgcc gtagcacccg ccgcagcaca taaccgcgcg attcattcga
     3121 cgggaaaata ccatccgaca tagcgaaagt gagagtgcgg atgtgatcag tcaacacgcg
     3181 caccgcaacc accgaagatc ccgaatactc tacgttaaaa acttcagccg ctttcatgcg
     3241 caaacgcatc agctcgtcgg tatcaaacac cgaatcgacg ccttgagtca acgtcgccaa
     3301 acgctcaagc cctgcacccg tgtcgatgcc ggtttgtttg agaggcaagt acgcgccgtt
     3361 ttggaaatcg aattggttaa acaccaaatt ccaaaactcc ataaagcgat cacccgagcc
     3421 gcccggtgct tggttttctt tgtcggtgcc gaactcttcg ccacgatcta aataaagttc
     3481 gctacacgga ccgcacgccc ccgcttcgcc ggcaggtccc caaaaattat cggcacgtcc
     3541 taaacgcaca attttttcgc gcggcacgcc aacgtgctta tgccaaatat ctaccgcttc
     3601 gtcgtcgttt tcaaaaacgc taatccaaat tttttcttta gggaaacgca atccttgcgt
     3661 cgagaattcc catgcaaact caatcgcttc ttttttaaaa taatcgccga aactaaaatt
     3721 gccgagcatc tcaaaaaaag aaaggtggcg tttagttttg cctactgatt cgaggtctgt
     3781 ggtgcgtaag catttttgca cggaaactaa acgcggtgac ggtggaactt tagccgcaga
     3841 aaaataatcc ttgaacggca ccatacccgc cgtggtaaaa agcaacgtgg gatccgccgc
     3901 tggcaaaagc gacgcgcttg gcacgcgcaa gtgtttttta ctttcaaaaa aagagagaaa
     3961 cgaattacga atttcattta ttttcatagt tgcttttatg gacgagagtt agttttatac
     4021 aacgaaacac gcgaacaagt atatataaaa aaggctgccc tcgcggtcag ccttttgtgt
     4081 tttttcgacg gctataaaat ttatttcttt gtagcctttt tctctttcgc cgacttgtcg
     4141 cttttggttg cgcttaccgc tctcttcttt ttgggaaggc gtgcagcgcg cactgctttg
     4201 cgatcgtctt tgagttgcgc caattctttt ctttcgacta attgaataat cgccatttca
     4261 gaagcgtctg aaacccgctg gccgacgcgc aagatgcggg tgtatccacc tgggcgcgaa
     4321 acaaaacggg gagcgatgtc gtcaaaaagc ttatgtaaaa tttctttatc ttttacaatt
     4381 tttgccgctt cgcgcaagtt atgcactttt tccgccgctg aaagcgcgct gttttggttt
     4441 tcgcgcgcac gggtgataag tttttcggca atttgccgtg tcacttttgc ttttgcaacg
     4501 gttgttgtga tgttctcgtg gtaaagaagc gattggacaa ggttacccaa catcgcttga
     4561 cgatggccga cttttttatt gagatgtttt actttattgc ctttattcat tgtatcctca
     4621 tatcttcaag cgtatgcttt tatctaccgt cgcgcatgcc aaatgcaaga ttcatattgg
     4681 caagcttact tttgagttgt aaaagcactt tatcggagta gtgcttgctt ttacgtaaat
     4741 cgtcttcgta acgggtgacg atatctttaa cgttgcgcat atccaaacta cgcagcacag
     4801 ctatcgtgcg caccgacatt tccatgtctt cgagcgcaat ctgcattgct tttttcaagc
     4861 gttcgtcggt ctcgtcgaac tcttcttcct cttcgtacat ttcttcagca aaattgatga
     4921 agatggttaa atgttctttg agaattttgg ctgcgtacgc cattgcatct tcgggcgaaa
     4981 tggtcatgtc cgtccaaatt tcaaaagtta ctttatcgta gtcggtacgt tgatcaacac
     5041 gcgtttctgt gatgtcaaag ttgacttttg taatcggtga aaataacgca tcgatgggaa
     5101 tcactcccac ttcatcgatc attttcttgg
//
LOCUS       DAOMPD010000167         5083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6915_length_5083_cov_2.806285, whole genome shotgun sequence.
ACCESSION   DAOMPD010000167 DAOMPD010000000
VERSION     DAOMPD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_5083_cov_2.806285"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLY93_06975"
     CDS             <1..539
                     /locus_tag="PLY93_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69257.1"
                     /translation="VYQLMVEPGTRLKERIRSKAIAAPNDDMQAKQMDAAITFLYEHN
                     FIRYEISNFAQKGFECRHNLLYWTNRPYLGLGVAAHGFTSKRRFYHPRSLEKYMLGLP
                     ALEDKEIQTHDPLINLLRLKRPIAIARIVSIVDDPVTAQRVLDEAIQKKWFNVSKGIL
                     TLTDEGLKFSDSLLSQLW"
     gene            complement(550..1179)
                     /locus_tag="PLY93_06980"
     CDS             complement(550..1179)
                     /locus_tag="PLY93_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRP69258.1"
                     /translation="MKRRGFTLVELLIVIGLTGFIFTTLMAVYMQVKKLSYEQTRVSS
                     QSAAVIDFGVAISYDLENLIFEKFNQRQIFNVTKELHGGHRFDAFAFPSASLNANPSV
                     LQARSFMVSYFVEPDKNGQDYILYRTEDMYIDARNPFRGVPVPIVRFVTEFRVEGSNN
                     GVDYEESWDFKTRRQVPRYIKITLRYRPTENSTEENEYVVDVRPPILWN"
     gene            complement(1176..1673)
                     /locus_tag="PLY93_06985"
     CDS             complement(1176..1673)
                     /locus_tag="PLY93_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRP69259.1"
                     /translation="MKRLTSVRRYLLRKKRRGFTLIEVLMALTLTAALSGLIIGSVVN
                     AMSMRQKALSLNKAITLAKKKMHEIKALNKTDSGRGDFVGMPGFKFEYLIKEEELDLQ
                     KMANEMGMGDAVADSPAAKYFKERNASAKSATGLAFRLLHFHVTVTWNEKDSYSLDYY
                     RGLGI"
     gene            complement(1670..2278)
                     /locus_tag="PLY93_06990"
     CDS             complement(1670..2278)
                     /locus_tag="PLY93_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRP69260.1"
                     /translation="MRARRGFTLIELLVVLVIGGSLLVLAYNLLASFMHERLSGNQDM
                     AFEGYFQDARRQALIQAKTLTLEVNFDKRTFGLREYDPKLELAPDAALKELAEIKRYR
                     YEREKDREFEEMSGKEKPQPKWIYSEQKLPTVLKKILSSSGLELAGPMVWIHFYPTGN
                     SDSVILQFGETAPNFVYIPRYNLSVVHLSEIRAFERQQEAPQ"
     gene            2362..3732
                     /locus_tag="PLY93_06995"
     CDS             2362..3732
                     /locus_tag="PLY93_06995"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRP69261.1"
                     /translation="MRQLAETQKWMVLSKIFVGIPVSIAYILRNFSAATTMTLTEYLA
                     SPSPAWAAENASTLLKSFGFEEATHAHDGVSNKLFARPFSGMLLAAHRPQKIDPAQLQ
                     LRIIGAHTDSPHLRLKSNSAAVKEGHTLLTSEVYGGALLYTWFDRPLGLAGEIFYKEN
                     NTIQRKLIRIQEPIAVVPSLAIHLARGVNDDGFKIEKTQNLHALLSLTEDISLDAYLA
                     SVADIPTDALLSYDLSLFDTTPATQGGLNNELLMSARLDNLVSAHSAITALKKADSPN
                     IIAIAALFDHEEIGSVSEAGAESVQLEKFLRRIQNHLKITDTQWEGVLQRSLFLSADM
                     AHGVHPNYADKSDTENRPRLGGGVTLKMNQNRRYATSAAGAAFFHDLCRTHKIPSQVY
                     THRNDLPCGSTLGPAVSARLGMTTIDVGVAMLAMHSIREICAIEDLESYRRFMEFFMA
                     KPLEFF"
     gene            3787..4485
                     /locus_tag="PLY93_07000"
     CDS             3787..4485
                     /locus_tag="PLY93_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRP69262.1"
                     /translation="MVKFFVNPSLLIALFLLFIFAPLRADKFVHPVDIARQMQAKFKT
                     LKTYQANFTLNIKDMNKTKVSSGVATYAEGGKLNFTFNQPAGDLIVSNGQMLYVYVSR
                     LRAVGKQPLKNKDKDGKPLFVAGTAEGLSNLFRRYHYRFDTPEQPRDVEGGKYFVLEL
                     KEKEITGGYDKITLFVKSDSYLIEKMHASSPSGRTVELTFSNIRLNPDIDPKLFQFKE
                     PDNAKIVDNPLTTE"
     gene            4488..>5083
                     /locus_tag="PLY93_07005"
     CDS             4488..>5083
                     /locus_tag="PLY93_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP69263.1"
                     /translation="MTAGALLKKTREEKRITLRQAADDTKITSRHLQAIEDDNYMVFP
                     GETYALGFLTSYAVYLDVDPDEVKRLYKGSQLTETETPLQELMQPTVQIGDYVERFAR
                     PLLIIPIAIVVVVGIIYLVSHLMSEKSSVDNTADNTTNSAVNFAKGSSAVPDVEADQV
                     KLTAGRPSPHIIKKGRAISFLIQNTEVFIVLRDISSDE"
BASE COUNT         1362 a         1279 c         1120 g         1322 t
ORIGIN      
        1 cggtgtacca gcttatggtt gagccaggca cgcgccttaa agaacgtatt cgctccaaag
       61 ccatcgccgc ccccaacgac gatatgcagg cgaaacaaat ggacgcggcg attacttttt
      121 tgtacgaaca taattttatc cgctacgaaa tatccaactt tgcgcaaaag ggttttgagt
      181 gccgccacaa cttgctttac tggacaaacc gcccgtactt ggggctaggc gttgccgcgc
      241 acggctttac gagcaaacgg cgtttttatc acccccgttc gttggaaaaa tatatgctgg
      301 gcttacctgc actcgaagat aaagaaatcc agacgcacga tccgttgatt aaccttttgc
      361 gcctcaagcg tcccatcgct atagcgcgta tcgtttcaat cgttgacgat ccggttactg
      421 cacaacgcgt tttggatgaa gctattcaaa agaaatggtt taatgtctca aagggaattc
      481 tcaccctcac cgacgaggga ctcaagttta gcgatagttt gctttctcag ttgtggtagt
      541 agggaggtgt taattccaca agataggcgg acgtacatcg accacgtatt cgttttcctc
      601 tgtggagttc tctgtaggtc tgtagcgtag agttattttg atataacgag gtacttgccg
      661 ccgcgttttg aaatcccacg attcttcgta atccacgccg ttgtttgagc cttcgacgcg
      721 aaactcagtc acaaagcgga cgataggcac tggaactcca cgaaaaggat tgcgagcgtc
      781 gatatacata tcttcggtgc ggtagagaat gtagtcttgg ccgtttttgt caggctcgac
      841 aaaataagaa accataaaac tacgcgcttg caataccgac gggttggcat tgagcgatgc
      901 cgatgggaaa gcaaatgcgt cgaaacgatg accgccgtgt aattcctttg taacgttaaa
      961 tatttgtcgt tggttaaatt tttcaaaaat taaattttca agatcgtaac taatcgcaac
     1021 gccgaaatca ataaccgccg ccgattgact ggagacgcgc gtttgctcgt aggaaagttt
     1081 tttgacttgc atatagacag ccatgagcgt cgtgaaaata aaaccggtga gaccaatgac
     1141 aatgagcaat tcgacgagag taaaaccacg ccgtttcata tccccagacc tcgatagtag
     1201 tcgagcgaat atgaatcttt ttcattccaa gtgacggtga cgtgaaaatg caaaagccga
     1261 aacgcaagac cggttgcgct tttcgccgac gcgttgcgtt ctttgaagta cttcgccgca
     1321 gggctgtcgg cgacggcatc tcccataccc atttcattgg ccattttttg taaatcaagt
     1381 tcttcttctt taataagata ttcaaacttg aatccaggca taccgacgaa atcgcctctg
     1441 ccgctgtcgg ttttattaag cgctttaatc tcatgcatct tttttttagc aagtgtgatg
     1501 gctttgttta agctcaatgc tttttgtcgc atcgacatag cgttcacaac gctgccgata
     1561 ataagccccg ataacgcagc ggtgagcgtg agagccatta aaacctcaat caaagtgaat
     1621 ccacgccgtt ttttacgcaa gagatagcgg cgcaccgagg ttaatcgttt cattgtggag
     1681 cctcttgttg cctttcaaac gcacgaattt cagagagatg cacaacagaa agattgtaac
     1741 gagggatata cacaaaatta ggcgctgtct cgccaaattg taaaatcacc gaatccgaat
     1801 ttccagtggg gtagaaatga atccatacca tgggccctgc cagctcaagg cctgagctgg
     1861 ataaaatttt tttcagcacc gtcggtaatt tttgctcgga gtaaatccac ttgggctgag
     1921 gtttttcttt ccccgacatt tcttcaaact cgcggtcttt ttcgcgttca tagcggtaac
     1981 gtttaatttc tgcaagctct ttgagggctg cgtcgggagc aagttctaat ttaggatcat
     2041 attcgcgcag accaaaagta cgcttatcaa aattcacttc gagtgtgagt gttttcgctt
     2101 ggatgagcgc ctggcggcgg gcatcttgaa aatacccttc aaacgccata tcttggtttc
     2161 cagaaaggcg ttcgtgcata aaacttgcaa gcaagttgta agcaagcacc aataacgacc
     2221 caccgatgac gagcaccacc aaaagctcga ttaaagtaaa accccgccgt gcgcgcattt
     2281 ctcagaccgc tatccttgct aattgcggcg ttcaatgtcg actctgtgcg acaactgtaa
     2341 agtttggaca gtgatgagcc tgtgcgccaa ctcgctgaga ctcaaaagtg gatggtttta
     2401 tcaaagatat ttgtgggcat ccccgtttct atcgcgtata ttcttagaaa tttctctgcg
     2461 gctacaacca tgaccctcac cgaatacctc gcctcccctt cccctgcatg ggcggctgaa
     2521 aatgcgtcaa cgttgcttaa aagttttggc tttgaagaag cgactcatgc gcacgacggt
     2581 gtatccaaca aactttttgc gcgacccttt tccggaatgc tccttgccgc gcatcgccca
     2641 caaaaaattg acccagccca actccaatta cgcatcatcg gcgcgcacac cgactcgcca
     2701 cacctgcgcc ttaagagcaa ctccgccgcc gtcaaagaag gccacaccct ccttaccagt
     2761 gaagtctatg gcggcgcatt gctttacact tggttcgacc gacctttagg tcttgcgggt
     2821 gaaatttttt ataaagaaaa taatacaatc cagagaaagc tcatccgtat tcaagaaccg
     2881 attgccgtcg tgccttcgct cgccattcac ttggcgcggg gtgtgaacga cgatggtttc
     2941 aaaattgaga aaacccaaaa tttacacgcg cttttgtcgc tcacggagga tatatccctc
     3001 gatgcgtatt tagcgtctgt ggcagacata cctaccgatg ctcttttaag ttatgatcta
     3061 tcgctttttg acacaacgcc tgctacgcaa ggcggattga ataatgaact actcatgtct
     3121 gcgcggctcg acaatttagt aagcgcacac tcagccatca cggcgcttaa aaaagcagac
     3181 tctcccaaca ttattgcaat cgctgcgctc ttcgaccacg aagaaattgg tagcgtctcc
     3241 gaagcgggcg ctgaaagtgt gcaacttgaa aaatttttac gccgcatcca aaaccatctt
     3301 aaaataacgg acacccaatg ggaaggtgtc ctgcaacgta gtctattttt aagcgccgac
     3361 atggcgcacg gtgtgcatcc caactacgcc gacaaaagcg acaccgagaa tcgtccccgt
     3421 ttgggtggcg gtgtcaccct caagatgaac caaaaccgcc gctacgccac gagcgcggcg
     3481 ggtgcggcgt ttttccacga cctttgccgc acgcacaaaa ttccatcgca agtttacacg
     3541 caccgtaacg atttgccgtg tggcagcacc cttggccctg cagtgagtgc acgtttgggc
     3601 atgactacca ttgatgtggg cgtggcgatg ctggcgatgc actccatccg tgaaatttgt
     3661 gcaatcgagg atttggagag ctatcgacgg tttatggagt tttttatggc taaacctctt
     3721 gagttctttt gatattcatt gacaccctgt ttcataagcg gcatcgttca attactacgg
     3781 ttatttatgg ttaagttttt tgtaaatcct tctttactta ttgctctttt tctccttttt
     3841 atctttgccc ccctccgcgc cgacaagttt gtgcaccctg ttgacattgc gcgccaaatg
     3901 caggccaaat tcaaaacatt aaaaacctat caagccaatt tcactttgaa cattaaagac
     3961 atgaacaaaa caaaagtcag ttcgggggtt gccacgtatg ctgagggggg taaactcaac
     4021 tttactttta atcagcctgc aggcgacctc atcgtttcca acggtcaaat gctctacgtg
     4081 tacgtttctc gtttaagagc ggtgggaaaa caaccgctca aaaataaaga caaagacggc
     4141 aaaccacttt ttgtcgccgg tacagccgag gggttatcga atctttttcg acgttaccac
     4201 taccggtttg atacccccga acaaccacgc gatgtcgaag ggggaaaata ctttgtttta
     4261 gagttaaaag aaaaagaaat tacaggtggc tacgataaaa ttacactttt tgtcaaaagc
     4321 gatagctatc tcattgaaaa aatgcatgca tcgagcccat cgggtagaac ggtcgaactc
     4381 accttttcga atatccgtct caaccccgat atcgatccca agcttttcca gtttaaagaa
     4441 cccgacaatg ccaaaattgt cgataacccc ctcacaacgg agtaaccatg actgcaggtg
     4501 cactactcaa aaagacacgc gaggagaaac gcattacttt acggcaagcc gccgacgaca
     4561 ccaaaatcac ttccagacac ttgcaagcga tcgaagacga taactatatg gtttttccgg
     4621 gtgaaacata cgcactgggt tttttaacaa gctacgccgt ctatctcgat gtcgaccccg
     4681 acgaagtaaa gcgtctctat aaaggttctc aactcaccga aaccgaaact cctctacaag
     4741 agttgatgca acccaccgtg caaatcggcg attacgtgga acgcttcgcc cgccctcttc
     4801 tcattatccc cattgcgatt gttgtcgttg tgggaattat ttatcttgta tcgcatttaa
     4861 tgtctgaaaa aagctctgtc gataacaccg ccgataacac aacgaactct gcggttaatt
     4921 ttgcaaaagg ttcaagcgcc gtgcccgatg tcgaagccga tcaagtcaaa ctcaccgcag
     4981 gacgccccag cccacacatc attaaaaagg gcagagcgat tagtttttta atccaaaaca
     5041 cagaggtttt tattgtactg cgcgacatca gttccgacga gaa
//
LOCUS       DAOMPD010000168         5034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_6995_length_5034_cov_3.757381, whole genome shotgun sequence.
ACCESSION   DAOMPD010000168 DAOMPD010000000
VERSION     DAOMPD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6995_length_5034_cov_3.757381"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1314
                     /locus_tag="PLY93_07010"
     CDS             100..1314
                     /locus_tag="PLY93_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP69264.1"
                     /translation="MAVIMLDKVDTQVFQEGELIFSEGDVSDFAYIIEDGEIEIFTVI
                     GNERKNLNLLTSGSLFGELALVDRQPRSASAVALKRTTLTLVTQQQVNERIQDSDPIL
                     RMLLFVVMKHFRSEVARARAQYAPFSRQDIIPDAEKFNQLRLTEAVNMVRLEADLRYA
                     LKEKQFRLYYQPIVELSTGRVTSVEALIRWNNPRRGLLSPADFIEIAEATKLILPIGD
                     WVIQEGLSAYQKFHHKVGSSISMSFNVARRQMENTDFLTKLAKDVEASSIDAKRIYLE
                     VLERNFLLRGKIDTWISECTIFGFPISLDDFGTGYSSLRYLQQFRPQVVKIDRSFIIG
                     LPDSNESRSICKAIIDLANALDIRVVAEGIETMNQAKALSELGCQFGQGYLFSKPVPE
                     IEFIHFLEKQAS"
     gene            complement(1369..4869)
                     /locus_tag="PLY93_07015"
     CDS             complement(1369..4869)
                     /locus_tag="PLY93_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP69265.1"
                     /translation="MNIAAFSIKRPIFVTSLVILMLSTGWISLHRIGVDLFPDVNVPF
                     ITITTTYAGAGPEEIETLISKKIEEEVASISGLKRVSSDNQEGVSLVMCEFYLGQDIK
                     EAEQQVRNKLGKVRRDLPDDIDEPLVQRFDPADQPIVRLSVAADLPPAKLYDYIDQKL
                     KPRFEQIANVGAVRISGGQKREIQVEVDRNLLNSNQMTLTWIANNLRSYGANVPVGKK
                     ETGDKEIAFRTMGRFEKLKQIENTVISFGGDVGSALLLKKLAKVYDGVEDAKSVAFLY
                     LPVDDDPLDLSGKPIDQSKMVRERRPAMVVDVYKQSNANTVAVAEATLKLMDKINEDL
                     RPQALRDLNTLKAMIRKGDKGDKLTAQVQKIQNEKFVKENANILTALKSLEAQVSKEP
                     RAPETSKILRELKKDINKVTAAPEISLIRNSARWIQINVDDVRETIIIGALLAVLVVY
                     LFLGNVRSTVITGLALPNSLLGAFILMLAMGFTINVITLLALSLSIGLLVDDAIVVRE
                     NIFRKMEEGEPSVKAAETGTQEVLLAVVATTMTVIAVFLPVGFLSGIVGQFLRQLGLT
                     VVFAMTISLFDAITMAPMLSAYFGGHVHEKPNVVIRYFNKFQNWLEAMYASIMKFTLR
                     RPLITLGLSFLVMIVNCGSCFTVKNTFFPPNTQPEFLVNFEMPPGTSLEGSKKMAEEI
                     ENRLSKLPEVHMIASVAGTYTGLANKGELGIVLVDRKWRKRETWQLKDVARNMLKDLS
                     YAKVSVNDYSAVGGGVMYPFNLNLSGENLDELEKYSVSVIEQLKKIPDFIDISSDYQT
                     GKPEFQIQLDPDRMQQVGVGPAAAGNELRLHVAGGVVGKFYDNGLEYDIRMRLREDQR
                     DLKTSFAKSYVPNLSQPSRPIPLNILGKGEVKTGPSNISRQDRSRVIKLHANLAPGGA
                     LGNAVQAAKKLIHEKAPPPEGVHLVFWGQAEDLKELMDNMVVAFGLALLFIYLVLASL
                     YESFITPVTILFAIPPAISGAFVSLAIFREQLNLFSIIGLIMLMGLVTKNSILLVDYA
                     LQRVRRGMGLNEAIFEAGKARLRPILMTSFAMIAGTLPLALGLGEAAKQRTAMGIAII
                     GGLVFSTIMTLFFVPAVFGYIDRFREWIEHRFRPDYDMTLVGNYGHDAHISMPKGNEG
                     KAASVLEEELKPAKRTRKKR"
BASE COUNT         1316 a         1164 c         1167 g         1387 t
ORIGIN      
        1 atacccccca attgcggcgt agcatttgtc aggtatgtcc gtgggttaga caatacataa
       61 aactatttta gatttacaag cgacaacaac cgctttatca tggcagtgat aatgttggac
      121 aaagtcgata cacaagtttt tcaggaaggc gaactcatct tttcagaggg tgatgtttcg
      181 gattttgctt acatcattga agacggtgaa attgaaattt ttacagttat cggaaacgaa
      241 cgaaaaaacc tcaacctctt aacctcgggc tctctttttg gtgaattggc gctcgtcgat
      301 cgtcaacccc gctccgcttc ggcggtggcg ttaaaacgaa caacgctcac tctggtgacg
      361 cagcaacaag tcaatgaacg tattcaagac agcgatccca ttttgcgcat gcttttgttt
      421 gtggtgatga agcacttccg ctctgaggtc gcccgcgcac gagcccagta tgccccgttt
      481 tcgcggcaag acattatacc cgatgcagaa aaatttaacc aactacgcct aaccgaagcg
      541 gttaacatgg ttcgacttga agccgacctc aggtatgcgc ttaaagaaaa acaattccgt
      601 ctttattatc aacccattgt cgagttatcg acgggacggg taacaagcgt tgaagcactc
      661 attcgatgga acaatcctcg tcgaggcctt ttatctcccg ctgattttat cgaaattgcc
      721 gaagcgacaa aacttatctt gcccattggc gattgggtta tccaagaggg attgtcggca
      781 tatcaaaaat tccaccataa agtgggttca agcatttcaa tgagctttaa tgtcgcgcgt
      841 agacaaatgg agaatacgga ttttctcaca aaacttgcca aggacgtcga agcgtccagt
      901 atcgatgcta aacgaattta tttagaagtg ctcgagcgta attttttact tcgtggtaaa
      961 atcgatacat ggatttctga atgtaccatt ttcggattcc ctatttcact cgacgatttt
     1021 ggtactggtt attctagtct tcggtattta caacaattcc gcccccaagt agtaaaaatt
     1081 gatcgttcgt ttataatagg tttgccagat agcaacgaaa gccgcagtat ttgtaaagcg
     1141 attatcgatc tcgcaaacgc gttggatatc cgtgtcgtcg ccgaaggcat tgaaacgatg
     1201 aatcaagcca aagcgttaag cgagttgggt tgtcaatttg gtcaggggta tcttttttca
     1261 aaaccagttc ccgaaattga gtttattcat tttttagaaa aacaagccag ctaaaaaatt
     1321 gttcagacgc gcctctgtca accagagcgc gtctgaatac tatcgcactt agcgcttttt
     1381 tctcgtgcgc ttggcaggct tcagttcttc ttccagaacc gacgctgctt ttccttcgtt
     1441 gcctttgggc atggagatgt gtgcgtcgtg gccgtagttg cccaccaacg tcatgtcgta
     1501 atcgggacgg aaacggtgtt caatccattc acggaaacga tcgatgtaac caaaaacggc
     1561 gggcacgaag aagagcgtca ttatcgtgga gaaaactaaa ccaccgatga tggcaattcc
     1621 catcgcagtg cgttgtttcg ctgcttcgcc taaacccaac gccaacggga gtgtacctgc
     1681 aatcatcgca aacgatgtca tgagaatggg gcgtagtcgc gctttacccg cttcaaagat
     1741 ggcttcgttg agccccatgc cgcgccgtac acgttgtaat gcgtagtcga ccaaaagaat
     1801 cgagtttttt gtcacgagtc ccatgagcat aatgagccca atgatactaa agaggttgag
     1861 ctgctctctg aaaatcgcaa gcgaaacgaa cgcgcctgag atggcgggcg gtatcgcaaa
     1921 gagaattgtc accggtgtga tgaacgactc gtagagcgag gcgagcacta agtaaataaa
     1981 gaggagtgca agaccaaacg ccaccaccat gttatccatg agttctttta aatcctcggc
     2041 ttggccccag aaaaccaagt gcacaccttc tggtggcgga gctttttcgt ggatgagttt
     2101 ctttgccgct tgtaccgcat tcccgagcgc ccctcctggc gcgaggttgg cgtggagttt
     2161 aattacacgg ctacggtctt ggcgtgaaat attcgacggt ccagttttaa cctcaccttt
     2221 acctaaaatg ttaagcggta ttgggcgaga aggctggctt aggttaggta cataactttt
     2281 ggcgaacgat gtttttaggt cgcgctggtc ttcccgaagt cgcatgcgga tgtcgtactc
     2341 aaggccgttg tcgtaaaatt tgccgacaac accacccgct acgtgtaaac gtaattcgtt
     2401 ccctgcggcg gcaggcccta cgcccacctg ttgcatacgg tcgggatcga gctgtatttg
     2461 aaactccggt ttgcccgttt gatagtcgga tgaaatatcg atgaagtctg ggattttttt
     2521 aagctgctct ataacgctca ccgaatactt ttcgagttcg tcgagatttt caccgctcaa
     2581 gtttaagttg aaaggataca tgacgccgcc acccacggcg ctatagtcgt tcaccgaaac
     2641 ttttgcatac gacaagtctt tgagcatgtt acgcgcaacg tctttgagtt gccatgtttc
     2701 gcgtttacgc cacttgcggt cgacgagaac aatacccagt tcgcctttgt tcgcaagacc
     2761 ggtgtacgta cctgcaaccg aggcaatcat gtgtacctcg gggagtttgg ataatcgatt
     2821 ctcgatttct tccgccattt ttttagaacc ttctaagctg gttcctggag gcatttcaaa
     2881 gtttaccaaa aactcaggct gagtgttcgg tggaaaaaat gtatttttaa cagtgaaaca
     2941 cgaaccgcag ttgacaatca tcaccaaaaa agaaagaccc agcgtaataa gagggcgtct
     3001 caaagtaaat ttcatgatgc tcgcgtacat cgcttcgagc caattttgga atttattgaa
     3061 atagcgaatc accacattgg gtttttcgtg aacatgccca ccaaaatatg cggagagcat
     3121 cggcgccata gtgattgcat caaaaagaga aattgtcatc gcaaagacaa cggtgagacc
     3181 cagttgtcgc aaaaattgcc ctacgattcc cgaaagaaat cccacaggta aaaaaacggc
     3241 aatcactgtc atcgtcgttg ctacaaccgc gagcaatact tcttgagtac cggtttcagc
     3301 cgcctttaca gaaggttcgc cttcttccat tttcctaaaa atgttttcgc gcaccacaat
     3361 cgcatcgtcg accaaaagcc cgatagacag cgagagcgca aggagagtga ttacgttgat
     3421 tgtgaatccc atcgcgagca tgagaataaa tgcacccaaa agtgagttgg gtagcgcaag
     3481 accggtaatg accgttgacc ggacgttgcc caagaataga tacactacca agactgctaa
     3541 aagtgcaccg atgataatcg tctcgcgcac gtcgtctacg ttaatttgaa tccaccgtgc
     3601 agagttgcga atgagtgaaa tttcgggcgc ggcggtaact ttattgatat cttttttaag
     3661 ctcgcgcaag atttttgaag tttcgggcgc acgcggttct tttgacacct gcgcctctaa
     3721 cgattttaac gctgtaagga tattcgcatt ttcttttaca aatttttcgt tttggatttt
     3781 ttgtacttgc gccgttagtt tatcgccctt atcgccttta cgaatcattg ctttgagcgt
     3841 atttaagtca cgtagagcct gcggacgtaa atcttcgttg attttatcca tgagcttcaa
     3901 tgttgcttct gcgacggcga ctgtgttggc gttcgattgt ttatagacat cgacaaccat
     3961 cgctggccta cgttcgcgca ccatttttga ttggtcgata ggtttaccgc ttaagtcgag
     4021 tgggtcgtcg tcgaccggca aatacaaaaa cgcgacagac tttgcatctt caacgccgtc
     4081 atagactttc gctaattttt tcaaaaggag tgcgctgccc acgtcaccgc caaatgaaat
     4141 cactgtgttt tcaatttgct tgagtttctc gaaacgaccc atggtgcgga atgcaatttc
     4201 cttgtcgccg gtttcttttt tgcctaccgg cacgttagcg ccatacgaac gtagattatt
     4261 agcaatccat gtaagtgtca tttggtttga gttcaaaaga ttgcgatcca cttcgacttg
     4321 aatttcacgt ttttgaccgc ccgaaatacg tactgcaccg acgttggcaa tttgctcgaa
     4381 gcgcggtttg agtttttgat cgatatagtc gtaaagttta gcggggggta aatccgcagc
     4441 gacagaaagg cgcacaatcg gttggtcggc ggggtcaaaa cgttgtacga gtggttcgtc
     4501 gatgtcgtcg ggtaaatcac gccgcacctt gcccaacttg ttgcgcactt gttgttcggc
     4561 ttccttgatg tcttgcccta gatagaattc gcacattaca agagaaacgc cttcttggtt
     4621 atcggagctc acgcgcttca aaccggaaat cgacgcgact tcttcttcaa ttttttttga
     4681 aatcagcgtt tcgatttcct ctggacctgc acccgcatac gttgtggtga tagtaataaa
     4741 aggaacgttt acatctggaa ataaatcgac accgatacga tggagggata tccaccctgt
     4801 gctcaacatc aaaataacga gactcgttac aaaaatgggg cgcttgattg aaaacgcggc
     4861 tatgttcata agtaataaaa taatctaaat taaacaagtt gacaaaaaaa actaaccgtt
     4921 tggttaattt acggtaacca gctgtgatat cggtattcag gaatttttgt ggttaggatt
     4981 ccatgaaaat aacgagtttg tccttcggcc aaggggtgat tgggacttcc tcgc
//
LOCUS       DAOMPD010000169         5031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7001_length_5031_cov_2.996182, whole genome shotgun sequence.
ACCESSION   DAOMPD010000169 DAOMPD010000000
VERSION     DAOMPD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_5031_cov_2.996182"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..395
                     /locus_tag="PLY93_07020"
     CDS             33..395
                     /locus_tag="PLY93_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69266.1"
                     /translation="MKNKMMKMKFGLAVIAFLGLLGTAVIAAPATYTSLQDIMLDGDD
                     AVGKIAELDMILVMLLSNGQIMATEPGSTDPITLTFKSKKIKDKLKKIRMNTSVHKVR
                     FKVYKDFGSTCGEILKID"
     gene            826..1599
                     /locus_tag="PLY93_07025"
     CDS             826..1599
                     /locus_tag="PLY93_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69267.1"
                     /translation="MTQIKKLIAFVLVLVGGTVYAKTYTFHFPPPKYEETCKEYTDLY
                     HAGKTVKAERLAKKYRKKYLNPVFYMQYDKPRIVKITGEGYWITKSSTDESYRFCYRE
                     LNSEDENNSFCGLSLFDIDSVKIPSNQLEKFQELIANNVSIHFASLTFEITNSGFGDG
                     ASLVTSDCFGSLSDSYSENPPRLSISNFIFGKTTLLNYTIDKSRKKKKKSSSDDDSEG
                     VSRESNDNEATEAENANREESHEAENSESGDTDHPTSEE"
     gene            1602..3092
                     /locus_tag="PLY93_07030"
     CDS             1602..3092
                     /locus_tag="PLY93_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRP69268.1"
                     /translation="MLDKAKIALEKTKTFAKENPKKTWGALGLGLVLLTVGGFALFYE
                     KPIPISEKKPYSVRIESYRTTKDADYAVDRLDSLGVDSYVVRRMGKQNDIWLDVHSGA
                     FDKKADATAFLKALKEKGLRESKVVAFKDIQKTIDAYKKAPSAERREYRLPSASIPKL
                     SKHILSSLKHFPIDKNYKIITVKFADSKTAPQSSLWRHFSFDYDYVPKGNTISKMMQN
                     TDAVAQAVYEDKLLRKRAGIIILACNDAKQTRENIQLVGEPKWGEKIASDLKFNTTHG
                     VLEGNLYEKKNKKATTLVFVGSFSKTNHVLTLVSHNLSREVFDKLLAAESDDTGLLVY
                     PEVRYNLSILPRDESGSVFTHFDLSRVGWSYAKQKGNAWWARNMVHHWNATGYFMKND
                     KPFSISFFNLNYGATADKIHQNFMSEKKKAKNTYSRELFKLMGVSPGWASKVREGTSG
                     WYLPKGSSSMNELSFALGSVVVSLDSYRQKVGEKEMKAIANDLQIW"
     gene            3105..4067
                     /locus_tag="PLY93_07035"
     CDS             3105..4067
                     /locus_tag="PLY93_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69269.1"
                     /translation="MKTITKTLIFLLGFSSIFALPEVDINTVRYHDYPEELKKAEFEK
                     GKQFYADLEENNKPLLDKMNTQPITDSTYYCANKAGRLEEFVNFVDSYLSKKNGLNDD
                     YEVVVEFNDVYVVRCSFLNFLNTYVSATIGVGLHIELDSTKMKSVNTKEVFDYDILRG
                     NINYIMPAIKNNNLEIIKFFACQAPYNDTWAKYKDGSKSYYERTLLMAATYGTIEAYI
                     VARECFFENTSMFTGYDKYDGYAYPVSFAIDDWSESKLEKYCNTYLDSAEKYEKSKKY
                     YLRKYDFCKSGAFFERSHTKKVPITQSEHKEIQKDYIFNGFKEE"
     gene            4334..4543
                     /locus_tag="PLY93_07040"
     CDS             4334..4543
                     /locus_tag="PLY93_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69270.1"
                     /translation="MQTNKLKLLALALAGLLLSALHAQETSAVDRMSDLLVKKSENKY
                     DRVIVIAIDGVPEYDAQKPFSKLVT"
     gene            4787..>5031
                     /locus_tag="PLY93_07045"
     CDS             4787..>5031
                     /locus_tag="PLY93_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69271.1"
                     /translation="MRDEFGSIVFNEPQFYAASNQSIVAQVKENYIDSPFTFIHFVDA
                     DNFAHYIGRKKIYSWAIQKEVEYSREIIEYIDTETEG"
BASE COUNT         1659 a          971 c         1113 g         1288 t
ORIGIN      
        1 tatgcaaaaa ataaatttga aagaggtaaa ttatgaaaaa taaaatgatg aaaatgaaat
       61 tcggtttagc ggtgattgct tttcttggtc ttttgggcac cgccgttatc gcggcacctg
      121 cgacttacac tagtctacag gatataatgc ttgatggtga cgacgctgtg ggtaaaattg
      181 ccgaactcga catgatactt gttatgctat tgagtaatgg ccaaataatg gcgacagagc
      241 ctggctccac agatcctata acattgactt ttaagagtaa aaaaatcaaa gacaaattaa
      301 aaaagattag gatgaacaca agtgttcata aggttagatt taaagtatac aaggattttg
      361 gttcaacttg tggagaaatt cttaaaatcg attaagccga ttatctaaaa agtcgaaagt
      421 ttgtattttt tggggtatta cccccaaagt cataggtggc ggtctctcgc ttcgggagcc
      481 gcgtcgaagt gtggtggggc caatttttat atgccgctca gatacgggtg tgggcgcgtt
      541 ttgggggtac gcggcggact tatcgaccgc tcttagagtt atgctttgga aaccgcgagc
      601 agcgaagcgc tttagcagga aatcgcaacg cgatttccga ggcttgcggt tttcagtgtg
      661 aactaaggac gataagttcg caagtctaaa gaacccccaa aacgcgttcg cacccctcag
      721 ataccgaaaa taactgcgtt ttatgcccgt ggaattattg tagcgcgcag cataaaagcg
      781 tgattttttt gcaataaaaa gcaccggtgc atacatagga gaagtatgac acagattaaa
      841 aaacttatag catttgttct tgtactcgtc ggcggtacgg tttacgcgaa aacgtacaca
      901 tttcattttc cgccgccgaa atatgaagag acttgtaaag aatatactga tctgtatcat
      961 gcaggtaaaa cggttaaagc agaacggctt gcaaaaaaat accgtaaaaa atatctcaat
     1021 ccggtgtttt atatgcagta cgataaaccc cgcatcgtca aaattaccgg cgagggctac
     1081 tggataacca aaagttcaac ggatgagtct tatagatttt gttatagaga actcaattca
     1141 gaagatgaga ataattcttt ctgcggtctt tctctttttg atattgattc tgtaaaaata
     1201 ccatcaaacc agttagagaa atttcaagag ttgattgcaa ataacgtaag tattcatttt
     1261 gcatcactca cttttgaaat aacaaattct ggttttggag acggtgcttc attagtcaca
     1321 agtgattgct ttgggtctct gagtgatagc tatagcgaga atccaccgag actttcaatc
     1381 agcaacttca tatttggtaa aacaaccctc cttaactaca ccatcgacaa gagcagaaag
     1441 aaaaagaaaa aatcatcaag cgacgacgat agcgaaggtg tgagccgcga gtcgaatgac
     1501 aacgaagcta cagaagctga aaacgcgaat cgtgaggaat cccacgaagc ggaaaatagc
     1561 gaaagcggcg acacagacca tccgacaagc gaggaataac catgttagat aaagcaaaaa
     1621 tagcactcga aaaaactaaa acctttgcaa aagaaaatcc caaaaagaca tggggcgcgt
     1681 tgggcttggg cctcgtgctg ctcacggtgg gcggttttgc cctcttctat gaaaagccga
     1741 tacccatttc agagaaaaaa ccgtactcgg tgcgtatcga aagttaccgc accacgaaag
     1801 atgccgacta tgcggtcgac cggctcgata gtttgggcgt cgacagttat gtcgtcagac
     1861 gcatgggcaa gcaaaacgat atttggctcg acgtgcatag cggtgcgttt gacaaaaagg
     1921 ccgacgctac ggcattctta aaagcgctca aagaaaaagg tttgcgcgaa agtaaagtcg
     1981 ttgcattcaa agacattcaa aaaaccatcg atgcgtataa aaaagcaccg tcggcagagc
     2041 ggcgcgaata ccgcctaccc agcgcctcga tacccaaatt gagcaagcac atactttcaa
     2101 gtctcaagca ttttccgatt gataaaaatt ataaaattat caccgtgaaa tttgccgaca
     2161 gcaaaactgc gccgcaaagc agtttgtggc ggcatttcag ttttgattac gactatgtac
     2221 ccaagggcaa cacgattagc aaaatgatgc agaacaccga tgccgtggcg caggcggtgt
     2281 acgaagacaa actcttgcga aaacgcgcgg gcattatcat tctcgcgtgt aacgatgcaa
     2341 agcaaacaag agagaatatc caactagtgg gagagcctaa atggggcgaa aaaattgcga
     2401 gtgatttgaa gtttaacacc acgcacggcg ttttagaagg caatctttat gaaaagaaaa
     2461 acaagaaggc aacaacgctg gtattcgtgg gctctttttc aaaaacgaac cacgtcctta
     2521 ctttggtgtc gcacaacctc agccgagaag tttttgataa actactcgcc gccgaaagcg
     2581 acgacaccgg ccttttggtt taccccgagg tgcgctataa tttaagcatc ttaccccgcg
     2641 acgaaagcgg ctcggtgttc acccacttcg acttatcgcg cgtcgggtgg agctacgcca
     2701 aacaaaaagg caatgcatgg tgggcgcgta acatggttca ccactggaac gccaccggct
     2761 actttatgaa aaacgacaaa ccatttagta tttcgttctt caatttgaac tacggcgcaa
     2821 cggcggataa aatccaccag aactttatgt cagaaaagaa aaaagcaaaa aacacgtatt
     2881 cacgcgagct tttcaaattg atgggagtct cccccggatg ggcgtcaaaa gtacgcgaag
     2941 gcaccagcgg ttggtatcta cctaaaggct caagctcaat gaacgaactc agcttcgcgc
     3001 tcggctcggt ggtggtttca ctcgattcgt acagacaaaa agtcggcgaa aaagaaatga
     3061 aagcgattgc aaacgattta cagatttggt gaggcggggg ggggatgaaa acaataacaa
     3121 aaacacttat ttttttactg ggatttagtt cgatttttgc actgcctgaa gtggatataa
     3181 acacagttcg gtatcacgac tatcctgaag aattaaagaa ggcggagttt gagaagggta
     3241 aacaatttta tgctgatttg gaagagaaca ataaaccttt attggataaa atgaatacac
     3301 aacctattac ggacagcaca tactattgtg cgaacaaggc aggtaggtta gaagagtttg
     3361 ttaattttgt tgattcgtac ctttctaaaa aaaatggatt gaacgatgac tatgaggtag
     3421 ttgtagaatt taatgatgtt tatgttgtcc gctgctcttt tttaaatttt ctcaatactt
     3481 acgtcagtgc gacaataggt gttggattac atattgagtt agatagtaca aaaatgaaat
     3541 ctgtaaatac aaaagaagtg tttgattatg atatactaag aggaaatatt aattatatca
     3601 tgcctgcgat aaaaaataat aatttagaga ttattaaatt ttttgcatgt caagcacctt
     3661 ataatgacac atgggcaaaa tacaaagatg gttcaaaaag ttactatgaa agaacattat
     3721 taatggcagc aacgtatgga acaatcgaag catatattgt agcaagagaa tgtttttttg
     3781 aaaatacgag tatgtttaca ggatatgata aatatgatgg gtacgcgtac cctgtatctt
     3841 ttgcaataga tgattggagt gaaagtaaac ttgaaaaata ctgtaataca tatcttgatt
     3901 ctgctgaaaa atatgaaaaa agtaaaaagt actatttgag gaaatatgac ttttgcaaat
     3961 cgggtgcgtt ttttgaaagg tcacatacta aaaaagttcc tattactcaa tcggaacaca
     4021 aagagataca aaaggattat atttttaatg gatttaaaga ggaataacct tttctacaac
     4081 agaaatagct attaacttat tcttttcagg cgggtacttg caagcagcga aagaaatgtt
     4141 tgtgcccgct gcatcttcgc gggcgtcgcc agcccaaagc tgcgtcgaaa actgctctcc
     4201 gtggtctctg tgtgctctgt gagaaaaaat cttacgactc atagaagaga ctctcgcaga
     4261 gaacgcggag cacgcaaagg aagaaacgca atttttttgc aataaaaatc atcggtgcgt
     4321 acatgagtaa tgtatgcaaa caaacaaact caagctgctc gctcttgcac tcgccggctt
     4381 actgctatca gcacttcatg cgcaagaaac aagtgccgtc gatagaatgt ccgatttatt
     4441 ggtaaaaaaa tctgaaaata agtacgatag agtcatcgtt atagcgattg acggcgtgcc
     4501 agagtacgac gcacaaaaac cattttcaaa acttgtaaca tagttacacg aaagagatta
     4561 caatacacaa tggaaaggca gtcgcgataa atgcactacg tcacaaccgt ataacttaac
     4621 cctaccggcg atggccaatt tttttacggg tgttgtggat catcgaatta agaacaatca
     4681 attttcagat aagttggcca agctaaccct ttttgacttg tatgaaaata gccaactgtt
     4741 tagctcttgg ccgcccctat taagaattat gagcaacgag caaagcgtga gagacgaatt
     4801 tggcagcatt gtgttcaatg aaccccagtt ttatgcggca agcaatcagt ctattgtcgc
     4861 ccaagttaaa gaaaattata tcgattcgcc tttcacgttt atacatttcg tcgacgccga
     4921 taatttcgca cattatatag gtcgcaagaa aatatattcg tgggcgatac aaaaagaagt
     4981 cgagtacagt cgtgaaatta tcgaatacat cgatacagaa acggaaggtg a
//
LOCUS       DAOMPD010000170         5025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7015_length_5025_cov_2.846278, whole genome shotgun sequence.
ACCESSION   DAOMPD010000170 DAOMPD010000000
VERSION     DAOMPD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7015_length_5025_cov_2.846278"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..861)
                     /gene="tmpT"
                     /locus_tag="PLY93_07050"
     CDS             complement(229..861)
                     /gene="tmpT"
                     /locus_tag="PLY93_07050"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HRP69272.1"
                     /translation="MEKEFWYQRWQNNQIGFHLGTTNPLLVKFFKRLELHANARVFVP
                     LCGKTRDIPWLLSRGFEVVGVELSPLAVEQFFEELGVKATRREDGPHFRYTSHQLTIF
                     QGDIFDLTPKSLGRVDAVYDRAALVALPPPMRHRYTQHLIELTHKAPQLLLTFDYDQS
                     KKEGPPFSVGEDLVRGEYGAVYTIELLHRKQLRDGLGGVSQAEECVWHLS"
     gene            981..2045
                     /locus_tag="PLY93_07055"
     CDS             981..2045
                     /locus_tag="PLY93_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69273.1"
                     /translation="MRSLFTGILIFLLAGASVSLLQAESETYPVRIIFEPRPDAKFYQ
                     IEWLENDDVHPTDKADPQKSRIEKITNVEIDRELETRFRYFRLRSAYRDGLYGPWGEV
                     VTITRSAKRPEKQNPTEENASTGPQTNDGTKNADSTPAEKNIHTEKAETSIPIEKAEN
                     TENQEPPAQQTQATGGIGAPRTYVKFYAPFIHGKGGFTVGGKTKIALRSQVTSPTVEK
                     INYRIYKEGADAGAWQQYENEIEVSSFSQNEYRYYTIEYQATSKSGIVETAQTRRMLI
                     DTTGPEIIEVSNENKAELQFSFKDENYPIVVRVYHNGKLIADKYFKFWNAHDIVKVPS
                     EQGLEIKAIDLLGNEAILKR"
     gene            2069..2467
                     /locus_tag="PLY93_07060"
     CDS             2069..2467
                     /locus_tag="PLY93_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69274.1"
                     /translation="MDIKEMVDKAFENKTLQEIRAAATSALQGVSEKGAEVLNQVLHA
                     KTVQDLAENKHVKNAQAIVNGSIQNLKDAVDKAWESKSLQEIANAGLEALQGISPDGV
                     AKLREILHVKTVKDLAENKHVQNAQAISKG"
     gene            complement(2511..4433)
                     /locus_tag="PLY93_07065"
     CDS             complement(2511..4433)
                     /locus_tag="PLY93_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP69275.1"
                     /translation="MWKFWVNNKVDTQEEIFSYANIWKLHVLNRTLWIVFVASLLSAA
                     IWFYRVRTFDLVFISTFLVALVTFVCLLPQLFWQFKAASLILALTGIGLVLLVVVGSH
                     YAPLATMGTATLMASFFFGRRFAYILVYSDSIIIAFLALLVHLGYLTTRDLIPPGERP
                     LTMWIQSGVVFLAMNLLLIKLAHEIVSSLEVSLDRLKRQHAISRNLTLYDSLTGLPNR
                     KLFYDRLEHAILSSERTLEYGALYLIDVDNLKNINDTAGHTSGDFFLTVVAERLSAAI
                     KEIDTVARLGGDDFAVIVENLSHTLSQAGREAERIGEKILKAINSPVSDPEDTTNIFQ
                     NTISIGLTLFYGRIHTAEELVKRADIALYQAKARGKNQLRNFDMVMQRKVEERIALET
                     DLKKALRNNELCLLLQPQYDTKHQIIGAEVLIRWVHKTRDVVYPSEFIPIAEDTGEII
                     EIGKWIIHTTCSQLHEWSKHPETQNLTISINVSPREFREPGYVDMIDATVRNAKIKPE
                     LLKLELTESLMLGDLEDTISKMERLREIGIGFSLDDFGTGYSSLSYLKRLPFSQIKID
                     QSFVRDLTTDKNGLILIRTIIGMAQNLNLHVIAEGVETREQLSVLTEMGCQTYQGFYF
                     SRPVSIPDFEKLLFLS"
     gene            complement(4462..>5025)
                     /gene="dctP"
                     /locus_tag="PLY93_07070"
     CDS             complement(4462..>5025)
                     /gene="dctP"
                     /locus_tag="PLY93_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRP69276.1"
                     /translation="FVYLMSKNKPISKYADMHGVKIWAPKGDPLVKAMLAEYGLVPVY
                     LGFEAVLPQLKTGGIDAFYAPPYGAIGLQWFREAKYLTNARLANAVGATLLAKSAYDR
                     LTNTQKQIVHSTLEKYAHTLTLELRRENEKSLKILLGKGIQKVEPNAEELSTLKSTAV
                     KVQNKLVGTLYSRELLEMARKARDSAK"
BASE COUNT         1506 a         1162 c         1086 g         1271 t
ORIGIN      
        1 ctttaacata tttgcctcta tatattaaat accagaaaac ggtcaaattt tctcactttt
       61 ctggaaaaaa gtgcaatttt tggtaaaaat tatatttttt ttgcagaaat cattcgtgtg
      121 gatagttatc ccctaatgtg acataatccc aaaaaacgag gatagttatc ccctaatgcg
      181 acataactca atagcgaatc gtccaccaga tagaatcttc aaaaatcttt aagataaatg
      241 ccaaacacat tcctcagctt ggcttactcc acccaagccg tcgcgcaact gctttcggtg
      301 caacaactca atcgtgtaca ccgcgccata ttctccacgc acgaggtctt cacccaccga
      361 aaatggcggc ccttcttttt tactttggtc gtaatcaaaa gtaagcaaca gttgcggtgc
      421 tttgtgcgtg agctcaatta agtgctgcgt gtaacggtgg cgcatcggcg gtggcagtgc
      481 aaccagcgca gcgcgatcgt acaccgcatc aacgcgcccc agcgacttgg gtgttaaatc
      541 aaaaatatcg ccttggaaga ttgttaattg gtgggatgta taccgaaagt ggggaccgtc
      601 ttctctacgg gtggctttga cgcccaactc ttcaaaaaac tgctccaccg caagagggct
      661 taactcaacc ccaacgactt caaagccgcg cgagagtagc cacggtatat cccgcgtctt
      721 gccacaaagc ggcacaaaaa cacgcgcgtt tgcatggagt tcaagccgct taaagaattt
      781 tactaaaagt gggttcgtcg tgcctaagtg aaacccaatt tgattatttt gccaacgttg
      841 ataccaaaat tccttttcca tagcaatacc ttctattcgt gcccaaccac acaatatgcg
      901 ttaatgaatt tgtgcgtcaa catcttctct atcgcttatt ttttaggttg tcacacaagt
      961 tcctttctca atcaaggaca atgcgttctc tttttactgg catcctgatt ttcttattag
     1021 caggtgcgag tgtttcgcta ttacaagcgg aaagtgaaac ctacccggta cgtattattt
     1081 ttgaaccacg ccccgatgca aaattttatc aaatcgaatg gttagaaaac gacgatgtcc
     1141 atcccacaga caaggctgat cctcaaaaaa gccgtataga aaagattaca aacgttgaga
     1201 ttgatcgcga actcgaaacc cgctttcggt attttcgatt gcgttcggcg taccgcgacg
     1261 ggctttatgg tccatggggc gaagtggtta caataacacg atcggctaaa agacctgaga
     1321 aacaaaatcc caccgaggag aacgcctcca cagggcccca aacaaacgat ggaacaaaga
     1381 atgccgactc cacaccagca gaaaaaaaca tccataccga aaaagcggaa acgagtatcc
     1441 ccattgaaaa agcagagaac accgagaatc aagaaccgcc agcgcaacaa acacaagcga
     1501 cgggtggtat cggtgcgccc cgcacctacg tgaaatttta cgcgccgttt atccacggca
     1561 aagggggatt caccgtcggc ggcaaaacga aaattgcgtt acgttcgcaa gtcacctcgc
     1621 ccactgtcga gaaaataaat taccgcatct acaaagaagg cgccgatgca ggagcttggc
     1681 aacagtacga aaacgaaatt gaagtgagtt cgttttctca aaatgagtat agatactaca
     1741 cgattgaata ccaagcaacg agtaaatctg gcattgttga aaccgcacag acacggcgca
     1801 tgctcatcga caccacaggc cctgaaatta tcgaagtctc caatgaaaac aaggctgaac
     1861 tacaattttc tttcaaagac gaaaactacc ccattgtggt gcgcgtgtac cacaatggaa
     1921 agctcattgc cgataaatat ttcaaatttt ggaatgcaca cgacattgtt aaagtgccga
     1981 gcgaacaagg cttagaaatt aaagccattg accttttggg caacgaagct atcttaaaga
     2041 gataagaaaa taagttaaga ggattacaat ggatataaaa gaaatggtgg ataaagcgtt
     2101 tgaaaacaaa acgcttcaag aaattcgtgc tgcagccaca agcgcattgc aaggcgtaag
     2161 cgaaaagggt gcggaagtgt taaaccaagt gctccacgcg aaaacagtac aagacttagc
     2221 tgaaaacaaa cacgtcaaaa acgcacaggc gattgtcaat ggttcgattc aaaacctaaa
     2281 agacgccgtt gacaaagcat gggaaagtaa aagccttcaa gaaattgcga acgcaggtct
     2341 ggaagcgtta caaggcattt cacctgacgg cgtggcaaag ttacgtgaaa ttttgcacgt
     2401 caaaacagta aaggacttag cagaaaacaa gcatgtgcaa aatgcacagg caatctcgaa
     2461 gggctaatag gttaataata tcttagtcag gatcgcttat gcggccatga ctaagataaa
     2521 aacaatagtt tttcaaagtc gggaatcgat actggccgtg aaaaataaaa tccttggtat
     2581 gtttggcaac ccatttcagt gagcactgaa agttgttcgc gggtttcaac cccttcggcg
     2641 ataacgtgta aattaaggtt ctgcgccata ccgatgatgg tacggatcaa tatcaaacca
     2701 tttttatcgg tggttaagtc gcgcacaaac gattgatcaa tttttatttg gctaaatggc
     2761 aaccgtttca aatacgaaag cgacgagtat cccgtaccaa aatcgtcgag actaaaacca
     2821 atgccaattt cacgcaaccg ttccattttt gaaatggtat cttcaaggtc gcccagcatg
     2881 aggctttcgg tgagttctag tttcaatagc tctggtttta tttttgcgtt gcgcacggtt
     2941 gcgtcaatca tgtcgacata gcccggttcg cgaaactcgc gcggtgaaac gttaatcgag
     3001 atagtcaagt tttgtgtttc gggatgcttc gaccactcgt gtagttgaga gcaagttgtg
     3061 tggataatcc acttgccgat ttcaataatc tcgccggtat cctcggcaat gggaataaat
     3121 tcgctgggat aaacaacgtc gcgggtttta tgaacccatc gaatgagcac ttctgctccg
     3181 atgatttgat gtttggtgtc gtattgaggc tgtaagagca ggcagagttc gttgttccgc
     3241 agcgcttttt tcaaatcggt ctcaagcgca atacgctcct caactttgcg ttgcattacc
     3301 atatcaaaat tgcgtaattg atttttaccg cgcgccttcg cttggtagag cgcaatgtct
     3361 gcgcgtttca ccaactcttc ggcggtatgg atacgcccgt aaaagagcgt taatccaata
     3421 ctaatggtat tttggaatat gtttgtcgta tcttctggat cgctcacagg gctattgatt
     3481 gctttcaaga ttttttcacc gatgcgctcg gcttcccgcc ctgcttgaga aagtgtgtgg
     3541 cttaaatttt ctacaatgac ggcaaaatcg tcgccgccta aacgagcgac agtgtcgatt
     3601 tctttaattg ccgcagagag gcgttcggcg acaacagtta aaaagaaatc gccgctggtg
     3661 tgaccggcag tatcgttgat gttcttcaaa ttatcgacat cgatgaggta aagtgcgcca
     3721 tattctaatg tgcgctcgct cgataaaata gcatgttcta aacgatcgta aaataattta
     3781 cggttgggca accccgtcaa gctgtcgtaa agcgtcaagt tgcggctaat cgcgtgttgt
     3841 cgcttcaaac gatcgaggct cacctctaag gacgaaacaa tttcatgcgc taattttatg
     3901 agaaggaggt tcatcgctaa aaaaacaacc ccagactgta tccacattgt caaaggtcgt
     3961 tcgccagggg gtattaaatc acgagtggtc aaataaccca aatgaaccaa tagcgctaaa
     4021 aaagcaataa ttatactatc cgaataaacg aggatgtacg caaaccgcct tccgaaaaaa
     4081 aaggaagcca taagtgttgc cgtacccatt gtcgctaaag gcgcatagtg agaacccacg
     4141 acgaccaaaa gcactaaacc aataccagtg agagctaaaa tcaacgaagc tgctttgaac
     4201 tgccaaaata attgcggtaa aagacagaca aaagtaacaa gagcgaccaa aaaggtcgat
     4261 ataaaaacta aatcaaaagt ccgtacacgg tagaaccaaa tagccgctga aagaagtgag
     4321 gcaacaaaaa caatccacag agttcggttg agaacgtgca acttccaaat gttcgcatag
     4381 ctaaatattt cttcttgcgt atcgacttta ttgttaaccc aaaatttcca cactggtttc
     4441 ccgcttaatc ttctttatct tttatttcgc actatccctg gctttacgcg ccatctcgag
     4501 cagttcgcga gagtagagag tccctaccaa tttattttgc actttcaccg cagtcgattt
     4561 gagagtcgat agttcttcgg cgttgggctc cactttttga atccctttac ctaaaagaat
     4621 tttgagcgac ttttcatttt cgcgacgcag ttcaagagta agtgtgtgcg catatttttc
     4681 aagagtcgag tgtacaattt gtttttgggt attcgtcaat cgatcgtaag ccgattttgc
     4741 caagagtgtc gcacccactg cgttcgctaa ccgtgcattc gtcaagtatt tcgcttcgcg
     4801 gaaccattgc aaaccaatcg cgccataagg cggcgcatag aacgcgtcga tcccccctgt
     4861 cttgagctgc ggcaacaccg cttcaaagcc caaatatacc ggcacaagac catactcggc
     4921 gagcatcgct ttgactagcg gatcgccctt cggcgcccaa attttcacac cgtgcatgtc
     4981 agcgtattta gagatgggtt tatttttgct cataaggtac acaaa
//
LOCUS       DAOMPD010000171         5023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7019_length_5023_cov_2.687802, whole genome shotgun sequence.
ACCESSION   DAOMPD010000171 DAOMPD010000000
VERSION     DAOMPD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_5023_cov_2.687802"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..352)
                     /locus_tag="PLY93_07075"
     CDS             complement(38..352)
                     /locus_tag="PLY93_07075"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRP69277.1"
                     /translation="MSESESRVNVELMGKTFTIKGDANPEYMADVANFVNVKIQEMRR
                     LTSGDTTKMLLLTALNIADELFQARRGIQVSPAEAEQIEKRTAALLRMLETGLVGDYR
                     QG"
     gene            complement(352..930)
                     /locus_tag="PLY93_07080"
     CDS             complement(352..930)
                     /locus_tag="PLY93_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69278.1"
                     /translation="MVPPVTITGQKTAIEKQIIGEQTELEKDVWMISSAKTAEHAQLE
                     SKPQEVRRSVEQENANTNRAFLKFEMFSKHLVALKKDRVVGENNKGFISNLIAEKVEI
                     PEEMKVKYSAENLDDPELGRPYRTLTQTVKEINEARVLAARGYIQNQKRVNKAFKATE
                     KEVLASQKDKYQQAALKGEMIQTENGEWVAKD"
     gene            complement(1010..1381)
                     /locus_tag="PLY93_07085"
     CDS             complement(1010..1381)
                     /locus_tag="PLY93_07085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRP69279.1"
                     /translation="MAVYSKGRISKGGGLQYEILKQGNGKVAQAGHRVQVHYTGWLNE
                     GGKKGKKFDSSVDRGRPFVFALGQGSVIKGWDEGVAGMKVGEKRTLFIPSALGYGARG
                     AGGAIPPHADLIFDVELLDVE"
     gene            1509..1982
                     /gene="ilvN"
                     /locus_tag="PLY93_07090"
     CDS             1509..1982
                     /gene="ilvN"
                     /locus_tag="PLY93_07090"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRP69280.1"
                     /translation="MKHVLSVKVNNTAGVLSHVTGLFTRRSYNIESLCVGATHDPQFS
                     VITLVIDADAAMIDQIKKQLLKLIDVIEIENLTGEISVKRELLLICIEIKKSERAELL
                     MLSRLFETSVIEMSDTQILLQMVAEPRRVMHFIEAIQPFKVLHMARTGVVALTLP"
     gene            1979..3088
                     /locus_tag="PLY93_07095"
     CDS             1979..3088
                     /locus_tag="PLY93_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69281.1"
                     /translation="MKKYFIALAVFITACGPSPEKFIEKAQQALAKEDYDAALLFMKN
                     AYEISLPKEFFITDRKLSFSFLQTSHDGKRVLLVEKKLTKNAYDPTKVFVIDREKEEE
                     KKCVIHGKIRDINFSPDGTAAVFLQQADEKSKDCTIWLWQITLDKTHEVGLTHCVTKP
                     AVKNDGTVWYLRLDQIHVFNPTTTKDTLFNQGRKPEKSAKKFPAYAYFYAAPDDKVWM
                     IYGAAGSYRLYQINTKTLKLVSKDIASNKLYLVAEDKIAGVFIGGAGNHEFVTLNPKN
                     SQISRRMKAKVWTDAAFVNKREYYYIEDGVLAHRKGDKETELPFWAEQIAIGAANELL
                     FLSSTGSAMHYANTMPPTESIKIYNKAVEIDDSKS"
     gene            3133..>3780
                     /locus_tag="PLY93_07100"
     CDS             3133..>3780
                     /locus_tag="PLY93_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRP69282.1"
                     /translation="MIFSEPRTSRAGLTHEAGEFLRLKERVKSIKASLKDIEYEKGVG
                     KMDAENADRLTEELLAEWDILEPKIAEYEKIPHTTTPQCPQCGTTILSQAAKFCHACG
                     SKLALLLLAVSLFLFTPRAILAYDIHVTVQNGTTEGVETTPLTIQLLRLSQGMEPVTT
                     KKSAAGKASFTNLPEIGNAPYMLQTVYKGVAYNRVIAPNIPSPADVKLEVFDSTAS"
     assembly_gap    3781..3790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3791..4433
                     /locus_tag="PLY93_07105"
     CDS             <3791..4433
                     /locus_tag="PLY93_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69283.1"
                     /translation="RVRTLVELRHTAKDELSGIMILFFLNSDRRAFVGGADGLEFALP
                     ENAKLEQASISVGSGSSNIQWLKLNPEKKTNGIYTVGQNVKPGDRVMQITFKMPYTQQ
                     KASLQFTPLYPQDTGLQLLVEPEDVLVRQGDKTLTRQHDKNLGRSLIAFNAAEKKVAF
                     QLSGGGIAPVQATEEVEVVVTSPLSLTQKLLFPVVAVFVFVCALFVMTKRREN"
     gene            complement(4430..>5023)
                     /locus_tag="PLY93_07110"
     CDS             complement(4430..>5023)
                     /locus_tag="PLY93_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69284.1"
                     /translation="YYKEPVTIEYYIPKESRFAYQEKLLYIPLYDPTPKNDNQILVMN
                     YFKNLSEPIDLMKVMDEYPQFLMKMLDYLSPQMGIVENLSRNIQYGLGGEGEAFRRAL
                     YTCEVLRKFEPSIVSLEIVGDYTTYNINWFVRKLNELKIDFSLEDPTVEFLIHRYRQF
                     ALEAGEVIPERFEILAEIYLDQAFPMEEDDFSDLIDN"
BASE COUNT         1357 a         1258 c         1097 g         1301 t
ORIGIN      
        1 agaactgaat aaattattaa gtaaatataa aaaataacta tccctgccgg tagtcgccga
       61 ccaatcccgt ctcaagcatc cgcaaaagtg cggcggtgcg tttttcaatt tgctcggcct
      121 ctgcaggcga gacctgaatt ccgcgccgcg cttggaaaag ttcgtcggcg atgttgagtg
      181 ctgtgagaag caacatttta gtcgtgtcac ccgaggtgag gcggcgcatc tcttgaattt
      241 taacatttac aaaattggca acgtcggcca tgtattcggg gttggcgtcg cctttaatgg
      301 tgaaggtttt gcccatgagt tcgacattga cgcggctttc ggattcactc attagtcttt
      361 cgccacccac tcgccgtttt cggtctgtat catttcaccc ttgagcgccg cttgttgata
      421 tttgtctttt tgcgaagcga gcacttcttt ctcggttgct ttgaacgcct tgttcacccg
      481 cttttgattt tggatatacc cgcgtgccgc gagaacccgt gcttcgttaa tttcttttac
      541 cgtttgcgtc agcgtgcgat atggtctacc taactcggga tcatccagat tttcagcgga
      601 gtatttaact ttcatctctt ctggaatctc tactttttcg gcgattaaat tggagatgaa
      661 gcctttgttg ttctcgccga cgacgcggtc ttttttgagc gcgactaaat gttttgagaa
      721 catttcaaat ttcaaaaaag cacggttggt gtttgcattt tcttgctcta cactgcgccg
      781 cacctcttgc ggtttgcttt ctaactgtgc gtgctctgcg gtttttgcag aggaaatcat
      841 ccatacgtct ttttcaagtt cggtttgttc gccgataatt tgtttttcaa tcgcggtttt
      901 ctggccggtg attgtaacgg gtggcaccac aatggtgctg ccgaaacacc gagtcagaga
      961 aaatagaacc ccaaccgcaa caaatccgcg taagggtgaa acacgcattt tactcaacgt
     1021 ctaaaagttc aacgtcaaaa attaaatcgg catggggagg gattgcacca cccgcaccgc
     1081 gcgcgccata acccagcgca gaaggaataa agagagtgcg tttctcgccg actttcattc
     1141 cggcaacgcc ttcatcccat cctttgatga ccgacccttg gcccaatgca aaaacaaatg
     1201 ggcggccacg atcgaccgat gaatcgaatt ttttgccttt cttgccacct tcattcaacc
     1261 aaccggtgta atgcacttgc acacggtggc ccgcttgcgc cactttacca ttaccttgtt
     1321 tcaaaatttc gtactgcaaa ccgcccccct tagagattct accttttgaa taaaccgcca
     1381 tagggataaa caccattgca gttattatag ctatccactt catagtcatg tattacaact
     1441 ttggcagtcg tgtcgagaat aaattgaagt tttctttacg atgcgtttcg cccacctaag
     1501 ctgccttaat gaaacacgtt ctttcggtta aggttaacaa cacagcaggg gttttaagcc
     1561 atgtcacggg tcttttcacg cgccgcagtt acaacatcga atcactgtgc gtgggagcaa
     1621 cgcacgatcc gcaattctcg gtgattaccc tcgtaatcga cgccgacgcg gcgatgatcg
     1681 atcaaattaa aaagcagttg ttaaagctca tcgacgttat tgagatcgaa aatcttaccg
     1741 gcgaaatttc ggttaagcgc gaacttcttt taatctgcat cgaaataaaa aaaagcgaac
     1801 gcgccgaact tttaatgctc tcgcgccttt ttgaaacgag cgtcattgaa atgtccgaca
     1861 ctcaaattct tttacagatg gtcgccgaac cacggcgtgt gatgcacttt atcgaagcaa
     1921 tccaaccgtt caaagtactg cacatggcgc gtacgggagt cgttgcgctc acgcttccat
     1981 gaaaaaatac tttattgctt tggcggtttt cattaccgcc tgcggtccat cgcccgaaaa
     2041 atttattgaa aaggcgcaac aagccctcgc gaaagaagat tacgatgcgg cgttgctctt
     2101 catgaaaaac gcgtacgaaa tttcattgcc gaaagaattt tttatcaccg atcgcaagct
     2161 atcgttttct tttttacaga cctcgcacga tggcaagcgt gttttgctcg ttgaaaaaaa
     2221 actcacaaaa aacgcttacg acccgacaaa agtttttgtc atcgatcgtg aaaaagaaga
     2281 agaaaaaaaa tgcgtaatcc acggcaaaat ccgcgacatc aatttctccc ccgacggcac
     2341 cgctgctgtt tttttgcaac aagccgacga aaaatccaag gattgtacaa tttggttgtg
     2401 gcaaatcact ttggataaaa cacatgaagt gggattaacc cactgtgtca caaagcccgc
     2461 cgtaaaaaac gacggcaccg tttggtactt gcggctcgac caaattcatg tatttaatcc
     2521 aacaaccacc aaggacacct tgttcaacca aggccgcaaa ccggaaaagt ctgcaaaaaa
     2581 atttcctgcg tacgcctatt tctacgctgc ccccgacgac aaggtgtgga tgatctacgg
     2641 cgcagcgggc agctatcgtc tttaccaaat caacacaaaa acgctcaagc tcgtttcaaa
     2701 agatattgcc tcaaacaaac tttatttagt cgcggaagat aaaattgccg gtgtcttcat
     2761 cggcggcgct ggcaaccatg aatttgtaac gctcaatccc aaaaactcgc aaattagccg
     2821 ccgcatgaaa gcaaaagtat ggacagacgc agctttcgtg aataaacgcg aatactatta
     2881 tatcgaagac ggtgttttag cgcaccgcaa aggcgataaa gaaaccgagc taccgttttg
     2941 ggcggaacaa attgccatcg gcgccgccaa tgaattgtta tttctctcat cgacgggttc
     3001 ggcgatgcat tatgcaaaca caatgccgcc gaccgagtcg atcaaaatct ataataaggc
     3061 cgtcgaaatc gacgatagta aatcatgatc tactttgttt ttgcgctcat tctggcagca
     3121 gccatttttt atatgatatt ttcagaacca cgcaccagcc gcgcggggct cacccacgaa
     3181 gcaggcgaat ttttacggct caaagaacgc gtgaaatcga ttaaagcttc gctcaaagat
     3241 attgaatacg aaaaaggcgt cggcaaaatg gacgctgaaa acgccgaccg cctcaccgag
     3301 gagcttctcg ccgaatggga catcctcgaa cccaaaattg ccgagtacga aaaaataccg
     3361 cacacaacaa cgcctcaatg cccccaatgc ggcacgacga ttttatcgca agccgcaaaa
     3421 ttttgccacg cgtgtggatc aaaactggcg cttcttttat tggcggtgtc tttattcctt
     3481 ttcacaccgc gcgcaatatt ggcgtacgat atccacgtta cggtgcaaaa cggcaccacc
     3541 gagggcgttg aaacgacgcc gttgaccatc cagctcttaa gactatcgca gggcatggag
     3601 cccgtcacaa caaaaaaaag cgcggcgggt aaagccagct tcaccaattt accagaaata
     3661 ggtaacgccc cttatatgct gcaaaccgtc tataaaggcg tggcgtataa ccgcgtgatt
     3721 gcgcccaaca tacccagccc cgccgacgtg aagctcgaag ttttcgactc gacggcgtcg
     3781 nnnnnnnnnn ccgcgtacgc acgctcgttg agttacgcca cacagccaaa gacgaacttt
     3841 ccggcattat gattcttttt ttcttgaata gcgatcgccg tgcatttgtg ggtggcgcag
     3901 atgggcttga atttgcgctt ccagaaaacg ctaaactaga gcaggcgtcc atctctgtgg
     3961 gatccggtag ctcaaacatc cagtggctca aactcaaccc cgagaaaaaa acaaacggta
     4021 tctacacagt tggccaaaat gtaaagcccg gcgacagagt gatgcaaatt actttcaaaa
     4081 tgccctacac ccagcaaaaa gcgtcattac agtttactcc actttacccg caagacacag
     4141 ggttgcaact tcttgtcgaa cccgaggacg tgcttgtccg gcaaggggat aaaacattaa
     4201 cgcggcagca cgataaaaac ttaggccgca gcctcatcgc atttaacgcg gcagagaaaa
     4261 aagtcgcctt ccaactttcg gggggaggta ttgccccagt acaagcaacc gaagaggtgg
     4321 aagttgtagt tacttcgccg ctttcgctta cgcaaaaact tctctttccg gttgtagcgg
     4381 tgtttgtgtt cgtctgcgcg cttttcgtta tgaccaagcg gcgagaaaat taattgtcta
     4441 tgagatcgga gaaatcatcc tcttccatgg ggaaagcttg atcgagataa atttcagcga
     4501 gtatttcaaa ccgctcgggg atgacctccc ccgcttctaa tgcaaattgc cggtaacgat
     4561 gtattaggaa ctcaaccgtt gggtcttcca gtgagaaatc aattttgagc tcgtttaact
     4621 tgcgcacaaa ccagttgata ttgtacgtag tataatcgcc cacaatttca agcgaaacta
     4681 tagacggttc aaatttacgc aacacttcgc aagtgtaaag tgcgcggcgg aaagcttccc
     4741 cctcgccgcc taaaccgtat tgaatgttac ggctcaaatt ttcaacgatt cccatttgcg
     4801 gcgaaaggta gtcgagcatt ttcatcaaaa attgtggata ttcatccatg actttcatga
     4861 gatcaatcgg ttcgcttaaa tttttgaaat agttcatcac caaaatttga ttgtcgtttt
     4921 tgggcgttgg atcgtaaagc ggtatataca agagcttttc ttgatacgca aaccggctct
     4981 ctttaggtat ataatactca atcgtcaccg gctctttgta ata
//
LOCUS       DAOMPD010000172         5018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7028_length_5018_cov_2.630062, whole genome shotgun sequence.
ACCESSION   DAOMPD010000172 DAOMPD010000000
VERSION     DAOMPD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_5018_cov_2.630062"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLY93_07115"
     CDS             <1..607
                     /locus_tag="PLY93_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP69285.1"
                     /translation="IDTQLTDALKKGAMLVATSKPTAHTKGGNFFPATLVINVTHKME
                     LMREETFGPILPVMKFDSEEEAIRLANDSTMGLTASVWTKNTKRAKRLAPRLQAGVVT
                     INDHLYTHGLSETAWGGFKQSGLGRTHGASGLEEMTQPQVVNWDLLTPPRNLWWFPFS
                     PETYDALKAALYFSSPLGVLNWIKSSLQLSVFAIKKMFGKG"
     gene            800..1569
                     /gene="tgt"
                     /locus_tag="PLY93_07120"
                     /pseudo
     CDS             800..1569
                     /gene="tgt"
                     /locus_tag="PLY93_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
     assembly_gap    1570..1584
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1585..1821
                     /locus_tag="PLY93_07125"
     CDS             <1585..1821
                     /locus_tag="PLY93_07125"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HRP69286.1"
                     /translation="NEKHARVDAPIDAECSCKVCRTHSIGYIRHLHKQKEILGLMLST
                     YHNLHFMYQFMKKMRQAIVEKRFAAFKQHWLSLF"
     gene            complement(1888..2106)
                     /gene="infA"
                     /locus_tag="PLY93_07130"
     CDS             complement(1888..2106)
                     /gene="infA"
                     /locus_tag="PLY93_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRP69287.1"
                     /translation="MAKEEAITVNGVIKKALPNAMFLVELENGHEILGHISGKMRKHY
                     IRINPGDKVSVELSPYDLTRGRITYRVA"
     gene            complement(2155..3051)
                     /locus_tag="PLY93_07135"
     CDS             complement(2155..3051)
                     /locus_tag="PLY93_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRP69288.1"
                     /translation="MRFKNEILLILAMLVWSAAWTSGKLVANSAPPALTLFWRYIFSC
                     AALLPFFPLLHESLHLSRRQLLWTFFSAAIMTAYSWLFFFGLKTGYAGAAGVLVTALN
                     PIFTYFLQRLIFRQVLTGRDKAGLLLGVVGGVILLEVWALSEEKILLSGNLLFLACAL
                     IYAFVTLATNATTKIVSTFVYSFYVNLFGAILLLPFIYNHFSETPPLQDFNYWGNIFF
                     LGVISNGFATTVYFYAVKNLGSAKASSYIFIVPAAALWMAAVYLGEPVNTTTIVGGVL
                     ALAAVALLLGVRVPTKRGRTAV"
     gene            complement(3055..4509)
                     /locus_tag="PLY93_07140"
     CDS             complement(3055..4509)
                     /locus_tag="PLY93_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69289.1"
                     /translation="MKRSVSLLCALSFFAVACSARQVRNETELQEAARLARAGDESGW
                     RMLENTVRQSDAEYFYRRSALKEIGKIPSSRAEPILKENLTNRELRGEAALGLVQQRN
                     EGNKQEIDQLIVSATDANLKTYNGLIREEIQALGEVDHPDAVRILQEQLRRDPNKDEA
                     VIDALGKILIRRKQSLYHPLDFTQPLGQQSNITTETLLSSDEVATAQPESAATTNDPE
                     KVLMEYLAGDGSVDTKDRAVQSIQEAHPPGVDYILRLIGQRSIPVKARIALVDYLTRL
                     AVNTQDKSMMDKLTRARSRAGNAQLVRSINLSLQVLGKAFGVRMAGVSYSRGTPSAPP
                     IKLPKEAEIIALKQKPYPGYSSGDVRTNLKKALAYYRLSGSLVERMESRVRSWLNAPE
                     NKEASERNLIFAALGRLYPNRDFYVLKAQGQDAFSRPGYFTTTLRLVTASSRGRSWQI
                     AVLQRLWGLTAQEADMLRQIYLRDGKLLEQRMRL"
     gene            complement(4527..>5018)
                     /locus_tag="PLY93_07145"
     CDS             complement(4527..>5018)
                     /locus_tag="PLY93_07145"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRP69290.1"
                     /translation="VKIDGQRAHRAVRKGETPVLKPRRVFVESLTLQKMSGGTLSMHC
                     TVSSGTYIRSLARDIAAALGTCGYLTALRRVAIGNFRLGDIAPEHPVGFLSQTLSDFD
                     ALYFFECLNLTPEESRKFLTGIRVRTTATDGIYRLQNGQVFLGLGRAEANLLRVEKVY
                     PTG"
BASE COUNT         1273 a         1303 c         1237 g         1190 t
ORIGIN      
        1 gattgacacc cagcttacag acgctctcaa aaaaggtgca atgttggttg cgacttcgaa
       61 accaacggcg cacaccaaag gcggtaattt tttccctgca acgctggtga tcaacgtgac
      121 ccacaagatg gaactcatgc gcgaagaaac ttttgggcca attttacccg tgatgaaatt
      181 tgattccgaa gaagaagcca tccgtctggc gaacgattca acgatggggc tcaccgcttc
      241 ggtttggaca aaaaatacca agcgcgcaaa aagactcgca ccgcgtctgc aagcgggcgt
      301 cgttactatc aacgaccatc tttacacaca cggtctttcg gaaaccgcat ggggtggttt
      361 caagcaaagc gggttgggac gcacgcacgg cgcctctggc cttgaagaga tgacacaacc
      421 gcaagtcgtt aattgggatt tactcacacc gccgcgcaac ttgtggtggt tccctttttc
      481 tcccgaaacg tacgatgcgc tcaaagctgc gctttatttt tcatccccat tgggtgtact
      541 gaattggatt aagtcatcgc tccaactttc agtgttcgcc atcaagaaga tgttcggtaa
      601 aggttaatat gctctgtgtt gccgagtgga cacaatgcaa tttacgatag aaaccaccga
      661 cggcgcagcg cgggcggggg tgttacaatt tcatcgtagt gccagctcgc agggaggtac
      721 atcctgtgcc actccccctc gcacagatgg cgtccgtgcc agttcggggg gaggcacctc
      781 ctgtgccacc cccctattca tgcccgttgg gacccaagca accgtaaaag ccatcaccca
      841 agaggatctt gctgccatcg gctatcaact catcctcggc aatacatacc acctctacct
      901 gcgcccaggc agtgacctca tccacggttt tggtgggttg cattccttca tggcgtggcc
      961 gcatgcgctc ctcaccgact ctggcggcta ccaagcattc tcactgagcc aaaatgtaaa
     1021 actcaccgac gaaggcgttc tcttccgttc gcacctcgac ggtagcgcac accacttcac
     1081 tcccgcaaac gttatccaaa ttgaacgaca aattggcgcc gacattatta tgcccatcga
     1141 cgattgcgcc ccctaccccg ccaacgagaa acgactcaaa gaaagtttgc tgcgcacaca
     1201 ccgttggcta tcccaatgca aagaagcatg gctcacagat aaaaggcacc aaagtctatt
     1261 cggcatttca caagggggga ttaaccctgt cctgcgcgca gaaagcgcaa aagcggtcgc
     1321 cgatttagat ttacccggct acgccgttgg tggcttgtcg gtcggcgaac cgttggagta
     1381 tttctaaccc gcgctcgcag aggcaatcga gcatttgcct gcggataaac cgcgttacgt
     1441 gatgggtgtg ggatcgatcc ccgaactcat ctatggcgtc gagttgggcg tcgatatgtt
     1501 cgattgcgta ctgccgacgc gtaacgcacg caacggccaa gtttttactt caaagggaaa
     1561 agtaaatttn nnnnnnnnnn nnnnaacgaa aaacacgcac gcgttgacgc gcccatcgat
     1621 gcggagtgct cgtgcaaagt gtgccgtacg cattcgatag gctatatccg gcatttacac
     1681 aagcaaaaag aaattttggg attgatgctc agcacttatc acaacttgca ttttatgtac
     1741 cagtttatga aaaaaatgcg gcaggcgatt gtagaaaaac gcttcgctgc atttaagcag
     1801 cattggttgt cgttgtttta agagcctgtg cgccaactcg ctgagacaat ttttttatac
     1861 acactgcgtg agttgccgca caggctctta agcgacgcgg tatgtgatgc gcccacgcgt
     1921 taaatcgtac ggagagagtt ccaccgacac cttgtcgcct gggttgatac ggatgtagtg
     1981 tttacgcatt ttgccggaaa tgtgacctaa aatttcatgg ccattttcta attccacaag
     2041 aaacatcgca ttgggcaatg ctttttttat aacgccgttg acggtaatcg cctcttcttt
     2101 ggccacacgg cattcgtgaa aattgagtca aggcgtcgaa atatatttct atttttatac
     2161 agcggtgcgg ccgcgttttg tggggacacg cacgccaagc aaaagtgcaa ccgccgcaag
     2221 cgcgagcacg ccgccgacga ttgtagtcgt gtttaccggc tcccccaaat acaccgccgc
     2281 catccacagc gcagcggcag gcacgatgaa aatatacgag ctggcttttg ccgaacccaa
     2341 attttttact gcataaaaat aaaccgtcgt agcaaagcca ttcgagatca cccccaagaa
     2401 aaatatattt ccccagtagt tgaaatcttg aagaggcggc gtctctgaaa aatgattata
     2461 gatgaaaggc aaaagcagaa ttgcgccaaa aagatttaca tagaacgaat agacgaacgt
     2521 cgaaacaatt tttgtcgtgg cgttggtagc gagtgtcacg aatgcgtaga tgagcgcgca
     2581 cgctaagaaa agtaaatttc ccgagagtag aattttctct tcggaaagag cccacacctc
     2641 aagaagaata acaccaccga cgacgcccaa gagcaacccc gctttgtcgc ggcctgtgag
     2701 tacttggcga aaaataagtc gctgtaaaaa ataagtgaag atgggattga gtgccgtcac
     2761 gagtacgccg gcggcaccgg cataaccggt ttttaatcca aagaaaaaaa gccacgagta
     2821 cgcagtcata atcgccgcag agaaaaaagt ccacagaagt tgccgtcgcg aaagatgcag
     2881 gctttcatgc aggagtggga aaaaaggaag aagtgctgcg cacgaaaaaa tataacgcca
     2941 aaaaagagtc agcgcagggg gagcactatt ggcgacaagt ttgcctgatg tccacgcggc
     3001 gctccacacg agcatcgcga ggatgagtaa aatttcattt ttgaagcgca caaactatag
     3061 acgcatacgt tgttctaaaa gtttgccgtc gcgtaaataa atctgacgaa gcatgtccgc
     3121 ttcttgtgcg gtgagtcccc acaaccgttg caacaccgcg atttgccacg acctgccgcg
     3181 cgaggatgct gtcaccaaac gcagcgttgt ggtgaaataa ccaggacggg aaaacgcgtc
     3241 ttgcccctgc gccttgagta cataaaaatc gcggttgggg taaagccgac cgagcgcggc
     3301 aaaaatcaaa ttgcgctccg acgcttcttt gttctccggc gcgttgagcc acgaacgcac
     3361 tcggctttcc attctttcga caagactgcc actcaaccga tagtacgcga gcgccttttt
     3421 gagatttgtc ctcacgtcgc cagatgaata cccagggtag ggtttttgtt ttagagcgat
     3481 tatttctgct tctttaggta attttatcgg aggcgcggat ggagtgcctc gagaataaga
     3541 tacacctgcc atacgcacac caaatgcttt ccccaaaact tgtagcgaca aattgatgga
     3601 gcgtacgagc tgtgcattgc ccgcacgcga acgtgcgcgg gtaagtttgt ccatcatcga
     3661 tttatcttgg gtgttcactg caagacgagt taaataatcc acaagcgcaa tgcgtgcttt
     3721 cacggggatg ctccgttggc caataaggcg caagatataa tcgactcctg gaggatgtgc
     3781 ttcttgaata ctttgcaccg cgcggtcttt ggtatcaacc gaaccgtctc ccgctaaata
     3841 ctccatgagc actttctcgg gatcgttcgt ggtggctgca ctctccggtt gtgccgtcgc
     3901 cacttcgtcc gacgacaata gcgtttcggt tgtaatgtta gattgttgcc ccaaaggttg
     3961 cgtaaagtcg agcgggtggt agagcgattg cttcctgcgg attaaaattt ttcccaatgc
     4021 gtcaatcaca gcttcgtctt tgtttggatc gcggcgtaat tgttcttgga gtatacgcac
     4081 agcatcgggg tgatcgactt cacccaacgc ttggatttcc tctctaatta atccgttata
     4141 cgttttcaaa ttagcgtcgg ttgcactcac aatcaactgg tcgatttctt gcttattgcc
     4201 ttcattgcgt tgctgtacaa gacctaacgc tgcctcgcca cgcagttcac ggtttgtgag
     4261 attttctttg agtattggtt ctgcccgtga cgatggaatt ttaccaattt ctttgagcgc
     4321 cgatctacga tagaaatatt cggcgtcgct ttgtcgcacc gtattttcga gcatacgcca
     4381 tccgctctcg tcaccggcac gggccaagcg agcggcttct tgtaattctg tctcgttgcg
     4441 tacttggcgg gcagagcagg cgacggcaaa aaagctcaac gcacacagta gcgatacact
     4501 ccgcttcata ttcttatatc ggcattttaa ccggtgggat aaaccttctc cacgcgcaaa
     4561 aggtttgctt ccgcgcgccc caatcccaaa aaaacttgcc cattttggag acgatatatc
     4621 ccgtcggttg cagtggtgcg cacacgaatg ccggttaaga attttcgact ctcttctgga
     4681 gttaaattta agcactcaaa aaaataaagc gcgtcgaagt cggaaagcgt ttgcgaaaga
     4741 aatcccacgg ggtgttcggg agcgatatcc cccaaacgaa aattaccaat cgcgacacgg
     4801 cgcagcgcgg ttaaataacc gcacgtcccc agcgccgctg caatgtcgcg cgcgagcgaa
     4861 cggatgtacg tgcccgagga aacggtacag tgcatggaga gcgtaccacc gctcattttt
     4921 tgtagcgtga gtgactccac aaaaacgcgc cgtggtttta acacgggcgt ctcgccttta
     4981 cgcaccgctc ggtgggcgcg ctggccgtct attttgac
//
LOCUS       DAOMPD010000173         4966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7124_length_4966_cov_2.607616, whole genome shotgun sequence.
ACCESSION   DAOMPD010000173 DAOMPD010000000
VERSION     DAOMPD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7124_length_4966_cov_2.607616"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1560)
                     /locus_tag="PLY93_07150"
     CDS             complement(268..1560)
                     /locus_tag="PLY93_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRP69291.1"
                     /translation="MKKGVRLCITGSEVMNGFVLDKNTQFFASELYALGYELKESRIL
                     PDNPDAIIRAWQELAQTGDIIVNSGGLGPTSDDLTVDLLCRWLDDVPRYDEYAEKRTR
                     YFFEKRAQREGEKTRDSVHKISLETALRQARLPSRAQPIKNNVGLAPGFYLPQIHFIA
                     LPGFPDEIKGMWKETLTLIESLGVEKSATKIIPIWGVGESQLFSMLQPHSEITLGVHA
                     LPWGCRLFLRSPSSQNAALDAYRATLEEKFPMQIMENPVVELMNLLKEKRLSLALAES
                     CTGGLAAKQVTDIAGSSAVFKGAAVTYANETKTALVGVSRKILEEHGAVSAPCAKAMA
                     EGASKALDADVTLSFTGIAGPTGGTPTKPVGTVFIGIHHRTEKMTRVARFQFPLGRER
                     FRNATVACGFLAATQMLTVNLQSTVQEKQAYWPARLFA"
     gene            1674..>4966
                     /locus_tag="PLY93_07155"
     CDS             1674..>4966
                     /locus_tag="PLY93_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRP69292.1"
                     /translation="MRFLRLLLFTILGFVYPLSALEIYINRAFTTTEKAEATCYASGE
                     GPLFLRVYRIDDVETYLTGQANAHTAEEKSERLMQPGYFLWRSVVDNFEYSLYQLARR
                     YMRSDYRNLLRDYLGLQKYQFPFRDRFPETNLFAPLAYPVVFEKKIQVRAQYWHSLKQ
                     QFESLKAGYYLVEISQGRHIAHAPLVISDIAMVTKISPYNFLVYAVDLLKNKPLSNAD
                     LSVYSREKSGKIEKHILKENFKLKDGLLFSNDVGYLKNSQSTLYVLRQGSHYAFSDIY
                     ALDTTKSLYESAIYTERPVYRIGDIVQVRAVFVRKQGAAAHGKVKYSIKKGDEILYTS
                     ESPLTEAGSLAFTFKTDTLKPARYTILMEIQGEKHYGNFLIEQYKKPETRANIRVDKN
                     ILLNGETINADLTAQYFSGEPLSKASVEVTLERMRISFPWWYGFSGSEYYGDWNDYAR
                     WDFVKEYTAVLDAKGKLKIPVATSEKGIEAPTDSDYIYRVRAKVKAQNREETQASARF
                     KVYRAPVQLRLTQNSWYFSAGAPITFNVNATSIYTDKPVSLRVQATLYQRTYDSKARR
                     YNDNAVASQEILLPNSGYKEAEFTGIKKSGTYLVRVSATDAGRTTTDTLETYVYGEEL
                     FYTDNSAERNISISADKKEYNLFDTAAFAIQMPSEKKIPALVVIENDRIRKFKLFESV
                     DASWIYKEELKNNLTPNFDISVLAFDYDKVPRFYSGQTQVVLPPQYRALTLEVTSDRE
                     RYRPGEEANFIVRTFDYQKKPVSAEFSLGVVDEAIYALHEDALKSLSLALNPRLTNSV
                     VTANSLAFSFYGYGTEKSLYALYRNQMAEAAALKKGERERLKIRKNFKDTAFFMANGK
                     TDASGLAKVHVPLPDNLTEWRITTHAHTRAGLSASGRTKITVSKDFALRLAKPRFLRE
                     RDEAQLRLLISNQLKEKQTAALEAQFENIKLVSKFPKTLDIPAGEEKYIDFKITNDTF
                     PKEGKARLQFIARTQSDNDGLEMELPLLPYGVENFIASQKIFAPNEKTWQSTLQLPNG
                     GRENLGTVNVSFTPGVLPSVHETLPYLIQYPYGCVEQTLSTFLPVLWASEASEKLKIP
                     LPVKKQQFDA"
BASE COUNT         1347 a         1137 c         1197 g         1285 t
ORIGIN      
        1 cttcttgggt gatggctttg tcggttaagc ctaaaaattt gcgtttgtcg atagcgtctg
       61 aagcgttcga gataagctca cgcaagaaaa tttctttgtt ggagtaaata gaatggatca
      121 tcaaatccat aagttgccgt gtttcggctt ggaatttaaa agtttgtgta ctcatagctc
      181 ttttgcgaaa agggaaaaaa ggacagggcg tcaactaaca ggctgttgcc atcgagcttt
      241 tttagagttg gcgcacaggc cgtagcttta tgcaaataac cgcgctggcc agtatgcttg
      301 tttctcctgc acagtacttt gcaaatttac cgttaacatt tgggttgccg ctaaaaaacc
      361 acacgccacc gttgcattgc ggaagcgttc gcgtcctaac gggaactgga aacgtgccac
      421 acgagtcatt ttttctgtgc ggtggtgtat cccaataaac acagtgccca caggtttagt
      481 gggagtgcca ccggtggggc ctgcaatccc ggtgaaggag agtgtgacat ctgcatcgag
      541 cgctttagaa gccccttccg ccatcgcctt tgcgcatggc gcactcacag cgccgtgttc
      601 ttccaaaatt tttcgactca cacccacaag agctgtcttg gtttcgtttg catacgtcac
      661 cgctgcaccc ttgaaaaccg cagaggaacc ggcgatgtcg gtcacttgtt tggcggccaa
      721 gccaccggtg cagctttccg ccaacgcaag cgacaagcgt ttttctttta ataaattcat
      781 gagttcaaca acggggtttt ccataatttg catcgggaat ttttcttcga gcgttgcgcg
      841 gtatgcatcg agtgctgcgt tttgtgaaga tggagagcgc aaaaaaagac gacaccccca
      901 cgggagtgca tgcactccca acgtgatctc ggagtgaggt tgcagcattg agaaaagttg
      961 gctttcccca acaccccaaa tcgggattat ctttgtggcc gacttctcaa cgcccaacga
     1021 ttcgatgagc gtgagcgtct ctttccacat acccttgatt tcatcgggaa agcccggtag
     1081 tgcaataaaa tggatttggg gaagataaaa tcccggcgca agccccacgt tatttttgat
     1141 tggctgcgcg cgcgagggga gacgcgcttg ccgcagcgct gtttcgagag aaattttgtg
     1201 cacactgtcg cgcgtttttt cgccttcgcg ctgtgcacgc ttttcaaaaa aatagcgcgt
     1261 gcgcttttct gcgtattcat cgtatcgagg gacatcatcc agccagcgac aaagtaagtc
     1321 gacggttaaa tcgtccgagg tgggaccaag tccacccgag ttgacgatga tgtcgccggt
     1381 ttgggcgagc tcttgccagg cgcggataat tgcgtcggga ttatcgggga ggatgcggct
     1441 ttctttgagt tcatacccca acgcatagag ctcggaggca aaaaattgtg tgttcttatc
     1501 gagaacaaaa ccattcatca cttcgctgcc cgtgatacac aaccgcacgc cttttttcat
     1561 aattactcgg tttgatttta gagcttaccc gacaactcta aagaattttg ttgtagtcca
     1621 ttcgaacagg cttctaagta attgacactg ccaagcagag ttcaaattac cgtatgcgat
     1681 ttctccgtct tcttttattt acaattcttg gttttgttta tcctctttcg gcgctcgaaa
     1741 tttatattaa ccgcgctttt acgacaaccg aaaaagcgga ggctacttgc tacgcttcag
     1801 gcgaaggtcc gctttttttg cgcgtgtacc gcatcgacga cgtagagaca tacttgacgg
     1861 ggcaggcaaa cgcgcacaca gcagaagaaa agagcgaacg tctgatgcaa cccggttatt
     1921 ttttatggcg gagtgtcgtt gataattttg aatactcgct ttatcaactg gcgcggcgtt
     1981 atatgcgttc tgattatcgc aatctgttgc gcgattattt aggtcttcaa aaataccaat
     2041 tcccgtttag agatcgcttt cccgagacaa atctctttgc gccgcttgca taccccgttg
     2101 tgtttgagaa aaaaatacaa gtccgtgcgc agtattggca ctcgctcaag caacaatttg
     2161 aaagtttgaa agcgggctat tatctcgttg aaatatcgca aggccggcat attgcccatg
     2221 caccgttagt aatttccgat attgcgatgg tgaccaaaat ctcgccgtat aattttttag
     2281 tgtatgcagt cgatcttttg aagaacaaac cactctcgaa tgcggattta tctgtgtatt
     2341 caagagagaa aagtggtaag atagagaagc atatactaaa agaaaatttt aaattgaaag
     2401 atggattgtt atttagcaac gacgtgggtt atcttaaaaa ttcacaaagt actctttatg
     2461 tgctgcgtca gggtagtcat tacgcgttct cggatattta tgcccttgat acaacaaagt
     2521 cgctttacga atcggccatt tacaccgaga ggcctgtcta tcggataggg gatatagtcc
     2581 aggtgcgtgc ggtgtttgtg cgtaagcaag gcgccgctgc gcatggcaaa gtgaagtatt
     2641 cgataaaaaa aggagatgag attctttaca cttccgaatc tccgctcaca gaggcgggga
     2701 gcttggcgtt tacttttaaa acggatacct taaagcccgc gcggtataca attctcatgg
     2761 aaattcaagg tgaaaaacat tacggcaact tccttatcga gcagtataaa aaaccggaga
     2821 cacgcgcgaa cattcgtgtg gacaaaaata ttttattaaa cggtgaaacg ataaatgctg
     2881 atctaaccgc gcagtatttt tcaggggagc cgctttccaa agccagcgtc gaggtaacgc
     2941 tcgaacgaat gcgcattagt tttccgtggt ggtatggttt cagcggctct gagtattatg
     3001 gagattggaa cgactacgcc cgttgggatt ttgtcaaaga atatacggcg gtattagacg
     3061 caaaaggaaa gttgaaaatc cccgttgcaa cgagtgaaaa agggatcgaa gcgcccaccg
     3121 acagcgatta tatttaccgc gttcgcgcca aagtcaaagc acagaaccgc gaggagacac
     3181 aagcatcggc gcgcttcaag gtgtaccgtg cgccagtcca gttgcgttta acgcaaaatt
     3241 cgtggtactt ttcggcaggg gcgcccatta ctttcaacgt caacgcgacc tcaatttata
     3301 cagataaacc cgtttcgctc cgcgttcaag caacactcta ccagcgcaca tacgatagta
     3361 aagcccgccg gtacaacgat aatgccgtgg cttcgcaaga aattttatta ccaaactcgg
     3421 ggtacaaaga agctgagttt acgggaatta aaaaaagtgg gacttacctc gttcgcgttt
     3481 cggcaaccga tgcggggcgc acaactacag acacgttgga aacctatgtt tacggcgaag
     3541 agttgttcta tacagacaat agcgccgaac gtaacatttc aataagcgcg gataaaaaag
     3601 aatataattt atttgatacc gcagcgtttg caatacagat gccgtccgaa aaaaagatac
     3661 ccgctcttgt cgtcattgag aacgatcgca tccgtaaatt taagttgttt gaaagcgtcg
     3721 atgcaagctg gatctacaaa gaagaattaa aaaataattt aacgccaaat tttgacatca
     3781 gcgttttggc gtttgactac gataaagtgc ctcgttttta ttcggggcaa acacaggtgg
     3841 tgttgccgcc gcagtaccgt gcgctcactc tcgaagtgac aagcgaccgc gaacgctatc
     3901 gcccgggaga agaagctaat tttatcgtac gcacattcga ttaccaaaaa aaaccagtga
     3961 gtgcagaatt ctcgctgggg gtggtcgacg aagctattta tgcgctccac gaagatgcgc
     4021 tcaagtcgct ttcgttggcg ctgaatccac gcctcaccaa ttctgtcgtg acagccaatt
     4081 cgctggcgtt tagtttttat ggctacggca ccgagaaaag tctctacgcg ctctaccgta
     4141 accagatggc agaagcggcg gccttaaaaa aaggcgaacg cgaacgcttg aaaattcgca
     4201 aaaatttcaa agataccgct ttttttatgg caaatggaaa aaccgacgct tcgggacttg
     4261 ccaaagtgca tgtgccgctg cccgacaatt tgaccgaatg gcgcatcacc acgcatgcgc
     4321 atacacgcgc gggtctatcg gcgagtggtc gcactaaaat tacagtgtcg aaagattttg
     4381 ccttgcgttt agctaaaccg cgtttcttac gcgagcgcga cgaagcacag ttgcgccttt
     4441 tgatctcgaa tcaattaaag gaaaaacaaa cagcggcgtt ggaagctcag tttgaaaata
     4501 taaaactggt atccaagttt ccgaagacgt tggatatccc agcgggggaa gaaaaatata
     4561 tcgattttaa gattacaaac gacactttcc ccaaagaagg aaaagcgcgt ttgcaattta
     4621 ttgcgcgtac ccaaagcgat aacgacggtt tagaaatgga actgccttta ttaccgtacg
     4681 gagttgaaaa tttcattgcg tcacaaaaga tttttgcgcc taacgaaaag acgtggcaaa
     4741 gcacgctaca attaccaaac ggtgggcgcg aaaatttagg aacagtgaat gtaagtttta
     4801 cccccggtgt gttgccttcg gttcatgaga ctctgcctta tctcattcaa tacccgtatg
     4861 gctgtgtgga gcaaacgctc tcgacatttt tgccggtgct ctgggcgtct gaggcgagtg
     4921 aaaaattgaa aataccgctg cccgttaaaa aacaacaatt tgatgc
//
LOCUS       DAOMPD010000174         4937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7171_length_4937_cov_2.312577, whole genome shotgun sequence.
ACCESSION   DAOMPD010000174 DAOMPD010000000
VERSION     DAOMPD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_4937_cov_2.312577"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>427)
                     /locus_tag="PLY93_07160"
     CDS             complement(<1..>427)
                     /locus_tag="PLY93_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69293.1"
                     /translation="EFRNQKEKDQDPKISLGVSPLPDGEVNIFSENTKHNLSYQGRVY
                     MKYVATGDKVKLDVGKTNEVVLRTFLRDFKRYAFTTDTHYDKTHYVKTWTDEYFYEHE
                     IENTLNYPVHVEIERTFEDMFVPGKIPFKYETVNTRTLK"
     assembly_gap    428..459
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<460..1285)
                     /locus_tag="PLY93_07165"
     CDS             complement(<460..1285)
                     /locus_tag="PLY93_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69294.1"
                     /translation="MSLFIRHKIQLALFLCILTPLAAHIELVTVPKSQAVELTIYNSA
                     DITMVKESRELTFKPGLNTIQFSWAGTLIDPTSLRLTFLTQKNKLNLRDTSFPPGRND
                     ALQWNIDSTLSGVARIEINYFTSGITWNADYTAITNTKETSMDVDGFIRVINNSGEDY
                     PNAKVRLVVGTVNLVEDIAGLAQGKWRYADLDEDRRDKVRNGFKRKMKRAEMSAGSVA
                     EEEMPAPAPQAKEIIKEGLSEYFLFTVEGKETIPTGWQKRLKAFSAKEVGVKVIYRA"
     gene            1371..>1916
                     /gene="selD"
                     /locus_tag="PLY93_07170"
     CDS             1371..>1916
                     /gene="selD"
                     /locus_tag="PLY93_07170"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRP69295.1"
                     /translation="MEIRLTEFSHGAGCGCKISPALLTDILRNNLVSSQANILVGNSD
                     RDDAAAYLWGEGQALLSTTDFFTPIVDNPYDFGRIAAANAISDIYAMGGRPIMALAIL
                     GWPIDKLSTATANEVIRGARDIAATCGITIAGGHSIDLPEPVFGLSVNGLVEAKHLKR
                     NSTVEVGDVLLLTKPLGVGIYS"
     assembly_gap    1917..1927
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1928..2434
                     /gene="selD"
                     /locus_tag="PLY93_07175"
     CDS             <1928..2434
                     /gene="selD"
                     /locus_tag="PLY93_07175"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRP69296.1"
                     /translation="AMKRQKLKAEHERLAIQTMVSLNKIGYALGKLAGVHALTDVTGF
                     GLAGHLLEMLEPRNCAAQIDFNALPLLPELQDYLAQGIFPGGAERNFKSFGHNISGID
                     DDYRRRIVCDPQTSGGLLIAAHPDAVDAIIRVLKEENPQSAVSVIGRITEQTDATRVN
                     FSPALTLA"
     gene            2474..>2818
                     /locus_tag="PLY93_07180"
     CDS             2474..>2818
                     /locus_tag="PLY93_07180"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRP69297.1"
                     /translation="MPLSRATFALIVANSIVFLITLSQSARFSEPSLQTLISFGANFA
                     PLTIFHGEVWRLFTSMFLHGGWIHLLVNMYSLYVIGAAVDFICGTKKYIMIYIVAGLA
                     GSVASVMYSLVRL"
     assembly_gap    2819..2828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2829..3097
                     /locus_tag="PLY93_07185"
     CDS             <2829..3097
                     /locus_tag="PLY93_07185"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRP69298.1"
                     /translation="VVSVGASGAIFGIYALLLTVMWKRNDLFRPEARRNVLKNGALVI
                     GYNLFIGFVAPEIDNAAHVGGFLAGATSGFIIAPKLRRPKLPQP"
     gene            complement(3217..4707)
                     /locus_tag="PLY93_07190"
     CDS             complement(3217..4707)
                     /locus_tag="PLY93_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HRP69299.1"
                     /translation="MKHKFLIFNVALLLSAGFALSAAPRRKAAAKKAPVTETTIAVEQ
                     KIAATPADDAKAKEAEMAKKYGISEELLESIRRKREPSETDLAKKKEGGYFTGLPLIN
                     SDPNTGVGYGVRLFYFNTGEKENPLFRRTPYRTQVYAQFFQTTLGYQYHELNWDAPYV
                     LNSLFRLRTNFVFEKNTWANFFGNGSRSMNALTSPSGTQYDKYADFNTDLRKISGGTT
                     NAAYHHYSYEKPTFNVMLERDMFGGIVRPQIGLQISKYSIWDLAGTKVKADGGDAINN
                     NTLLTQYQNAGLLVGRNGGWYNTARVGVAIDTRDYEPDPNSGQLFEAVAEVSNQALGA
                     ATNFARYTLSERVFYSPFPKLADLVIAGRGVYAQSVGDVPFYAMNQFSSTERVYLGAL
                     GGLRSLRGYKDNRFVANNIAIANLELRWTTFDFRVLGQNFAPILVPFFDIGSAFDQPK
                     DISKSQWRYAYGMGLRIAWNQATIIMVDYAMSKEDSNLFINFNHIF"
BASE COUNT         1234 a         1201 c         1121 g         1328 t
ORIGIN      
        1 aatttgagtg tgcgtgtatt gacggtctcg tacttaaagg gaatttttcc tggcacaaac
       61 atgtcctcga aggtgcgttc gatttcaacg tgcacggggt agttcagcgt attctcaatt
      121 tcgtgttcgt agaaatattc atccgtccac gttttcacgt aatgcgtttt atcgtaatgc
      181 gtgtcggtgg tgaaagcata acgtttaaag tcgcgcaaaa aagtgcgcaa caccacttcg
      241 tttgttttac ctacgtcgag tttgacctta tcgccagtgg ccacgtactt catataaacg
      301 cggccttggt aactcaagtt atgttttgta ttctcggaga aaatattcac ttcgccatcg
      361 ggcaaaggcg acacacccaa actaatttta gggtcttgat ccttttcctt ttgattccga
      421 aattcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ggcgcgataa atcactttta
      481 cacccacctc cttggcgctg aacgctttaa gacgtttttg ccaaccagta gggattgtct
      541 ctttgccttc gacggtaaaa agaaaatatt cagaaagtcc ttctttaata atttctttcg
      601 cttgtggcgc gggtgcgggc atttcttcct ccgctaccga gcctgcagac atttcagcac
      661 gcttcatttt tcgcttaaag ccattacgta ctttatcgcg gcggtcttcg tctaaatcgg
      721 cgtaacgcca ttttccttgt gccagaccag caatatcctc gacaagattc accgtaccca
      781 caaccaagcg cacttttgca ttgggataat cctcgcccga attgttgatc acccgaataa
      841 atccgtcgac atccatgctt gtttcttttg tatttgtgat cgctgtgtaa tcggcattcc
      901 aagtgatacc ggatgtgaaa tagttaatct caatacgcgc gactcccgaa agcgtactgt
      961 cgatgttcca ctgcaacgcg tcattacgcc caggaggaaa cgaggtgtcg cgtaagttaa
     1021 gcttattttt ttgcgttaaa aaagtaagac ggagggatgt gggatcgatc aacgtccccg
     1081 cccacgaaaa ctggatggta ttgagcccag gtttaaacgt tagctcgcgc gattctttca
     1141 ccatggtgat gtcggcgcta ttatagatgg tgagttccac cgcttggctt ttgggcacag
     1201 tgacgagctc aatgtgcgcg gcaaggggcg tcaagataca gaggaataga gcaagctgta
     1261 ttttatgtcg aatgaataaa ctcatattct cttagacgct actggcaaca aaaagttcaa
     1321 attattcgac aacgcataag cgtatttaat taaatccttg agcacctttt atggaaatcc
     1381 gtctcactga gtttagccac ggcgccggct gtgggtgtaa aatttcacca gcgctcctca
     1441 ccgacatctt acgtaacaat cttgtcagct ctcaagcaaa cattcttgtc ggcaactccg
     1501 atcgcgacga tgcagcggcg tatctttggg gcgagggaca agcgcttttg agcaccaccg
     1561 atttttttac ccccatcgtg gacaatccat acgattttgg aaggattgct gcggcgaatg
     1621 ccatttcaga tatttatgcc atggggggcc gccccatcat ggcgttggca attttgggtt
     1681 ggcccatcga caaactttct acagcaacgg cgaatgaagt gattcgaggc gcacgcgata
     1741 ttgctgccac atgtggaatc acaattgcag gcggtcacag cattgattta cccgaaccgg
     1801 tgtttggtct ttcggtaaat ggccttgtgg aagcaaaaca cctgaaacgg aattcgaccg
     1861 ttgaagtggg ggatgtcctc ttgctcacca agcctttggg cgtgggtatt tattccnnnn
     1921 nnnnnnngcg atgaaacggc aaaaattgaa agcagaacac gaacgcttgg ccatccaaac
     1981 gatggtatct ctcaataaaa taggttatgc actcggtaag ttagccggcg tgcatgcgct
     2041 caccgatgtc accggctttg gattggcagg ccatctattg gaaatgctcg aaccgcgcaa
     2101 ctgtgccgcg caaatcgatt tcaacgcgtt gccactcttg cccgagcttc aagactatct
     2161 cgcgcaagga atttttcccg gcggcgccga gcgcaatttt aaaagtttcg gccacaacat
     2221 ttcaggtatc gacgacgatt accgccgccg catcgtgtgc gacccccaaa cctcgggtgg
     2281 acttttaatc gccgcccatc ccgatgctgt cgatgcaatc attcgcgttc ttaaagaaga
     2341 aaatccacaa agcgccgtga gtgttattgg tcgcatcaca gaacaaaccg acgctacgcg
     2401 ggtgaacttc tcgcccgctc ttacactcgc ttaaaccact ttacacgaca cactattctc
     2461 tctctataaa atcatgccac tttcgcgtgc tacttttgcc ctcatcgttg ctaactccat
     2521 tgtgtttctg atcacgcttt cgcagagcgc gagattttca gaaccaagcc tccaaactct
     2581 gataagcttt ggcgcaaact ttgctccgct caccattttc catggcgaag tgtggcggct
     2641 tttcacatcg atgtttctgc acggcggatg gatacacctg ctcgtcaata tgtactcgct
     2701 ttacgttatc ggagcagcgg tggatttcat ctgcggcaca aaaaaatata ttatgattta
     2761 tatcgttgcc ggtcttgcgg gtagcgttgc aagtgtgatg tactctctgg tgcgcttann
     2821 nnnnnnnncc gtggtgagcg tgggggcatc gggcgcaatt ttcggtattt acgctctact
     2881 cctaacggtc atgtggaagc gcaacgatct attccgcccc gaagcgcggc gcaatgtgct
     2941 taaaaacggc gcactggtca ttggctataa cttattcatt ggttttgttg cgcccgaaat
     3001 cgacaacgcc gcgcacgttg gcggtttttt agcgggcgca acttcaggat ttatcattgc
     3061 tccaaaactc cggcggccta aattaccgca accttgacgc tgtgtatgag catcgtgccc
     3121 acaagaataa aaaaccggtg agcaaatttt actgcaacat catttgagga atcccattaa
     3181 gcgttgcagt aaaaaaaggc atctgatgga ggaatattaa aaaatatggt tgaagttaat
     3241 aaagagattt gaatcttctt tactcattgc gtaatccacc ataataatcg tcgcttggtt
     3301 ccacgcaatc cgtagtccca taccatacgc ataacgccat tggctttttg aaatatcttt
     3361 aggttgatcg aacgcgctac caatgtcaaa aaacggcacc aaaatcggcg caaagttttg
     3421 accaagcact ctaaaatcaa acgtcgtcca acggagttct aagtttgcaa tcgcaatgtt
     3481 attggcaaca aaacggttgt ctttgtaacc acgcagcgaa cgtaaaccgc ctaacgcgcc
     3541 caagtaaaca cgctcagtgc tcgagaactg gttcatggcg tagaagggaa catctcccac
     3601 agactgggca tacacaccac gccctgcaat aacgagatcg gcaagtttag ggaatggact
     3661 atagaatacg cgctcagaga gtgtataacg cgcaaaattc gtcgccgctc ccagcgcttg
     3721 attagagacc tcggcaaccg cttcaaaaag ctggccgcta ttgggatctg gttcgtagtc
     3781 gcgggtatca atagcaacgc ccacacgcgc ggtgttgtac cagccgccgt tacgaccgac
     3841 aagcaggcca gcattttgat attgagttaa gagcgtgttg ttattaatcg catcgccacc
     3901 gtcggcttta acttttgtac ccgctaaatc ccagatggaa tacttgctga tttgcaaccc
     3961 aatctgtggg cgcacaatac ccccgaacat gtcgcgttca agcatcacgt tgaacgtggg
     4021 tttttcatac gagtaatgat ggtatgcagc attggtggtg ccgccactaa ttttgcgcaa
     4081 atccgtatta aagtcggcgt atttgtcgta ttgagtacca gatggactgg taagcgcatt
     4141 catactgcgc gagccgttac caaaaaagtt tgcccatgta tttttttcaa aaacaaaatt
     4201 ggtgcgcaac cgaaacagtg aatttaagac atacggcgca tcccagttga gttcgtggta
     4261 ttgatacccc aatgttgttt gaaaaaactg tgcgtagact tgggtgcggt acggtgttct
     4321 gcggaaaaga ggattttctt tttcaccggt attaaaatag aaaagacgaa ctccataccc
     4381 gacacccgtg tttgggtcgg agttgatgag cggtaaacca gtaaaatatc caccttcttt
     4441 cttcttagcg aggtcggttt cgcttggctc acgcttacgg cgtatcgact caaggagttc
     4501 ttcagaaatg ccatattttt tcgccatctc tgcttcttta gctttcgcgt cgtctgccgg
     4561 tgttgctgca attttttgtt caaccgcaat ggttgtttct gttacaggcg cttttttagc
     4621 tgccgccttt ctccgcggcg ccgcactgag cgcaaaccct gcggacaaca agagcgctac
     4681 gttaaaaatc aaaaatttat gtttcattaa atcctccctt ttgagtaaag acggctgtta
     4741 ttcaatcggt gtttcgtcaa caagaaaggg agagaatctt acgagcctaa gagcctgtgc
     4801 gccaactcgc gcagcgtgta caaaaatttg caaaaaaatt gtctcagcga gttggctcac
     4861 atgccctaac gcttctatac aatttgtcta acccacggac atacctgaca aatgctacgc
     4921 cgcaattggg gggtatt
//
LOCUS       DAOMPD010000175         4931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7184_length_4931_cov_2.124077, whole genome shotgun sequence.
ACCESSION   DAOMPD010000175 DAOMPD010000000
VERSION     DAOMPD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7184_length_4931_cov_2.124077"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>636
                     /locus_tag="PLY93_07195"
     CDS             <1..>636
                     /locus_tag="PLY93_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRP69300.1"
                     /translation="HLEKEKLGERRVQYKLRDWLFSRQRYWGEPVPISFTADGTMVAE
                     TEENLPLELPESQNFQPSPSGESPLAHLTDWVNFTQNGAALRRETNTMPQWAGSSWYY
                     LRYADPHNTERFVSKEAEVYWLKDGVDLYVGGAEHAVLHLLYARFWHKVLYDLGYVTS
                     IEPFKKLGHQGIILGEDGSKMSKSRGNVINPDTVVAEYGADAFRLYEMFMG"
     assembly_gap    637..646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <647..1233
                     /locus_tag="PLY93_07200"
     CDS             <647..1233
                     /locus_tag="PLY93_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRP69301.1"
                     /translation="QMKPWSSKSIEGIYRFLARVYRLYLTDNAINKKLLAEPKDKKAI
                     LKILHQTIKKVTQDIESLSLNTAISQMMIFVNEAYKLESIGRDAASTFLQLLHPFAPH
                     LTEELWQRLGYSGGLLQSVWPQFDAAHLTEDEFEAVIQINGKIRARQKMPMGISDADF
                     ETAARALDSVKAALEGKPARKVIVVKNKMINFVV"
     gene            1289..1813
                     /locus_tag="PLY93_07205"
     CDS             1289..1813
                     /locus_tag="PLY93_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRP69302.1"
                     /translation="MQKQQRRDEFKILARTFFEKASSKDLEILHQKIIQLIERYTEKF
                     FSSGETLPKILGIYKPLRYELPVKEMIARVPQLNEVPLAYPEYNPQEMWFVKEDNGTH
                     CAPDFFIVPGLFVDRNGYRLGRGKGYYDRYFAAHPTPLPRRVFLGYPFQFIDAVPHDV
                     HDVTVVPLSPVNSL"
     gene            complement(1800..2216)
                     /locus_tag="PLY93_07210"
     CDS             complement(1800..2216)
                     /locus_tag="PLY93_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRP69303.1"
                     /translation="MNTLQEELTNESMEVESSSPETTQKKTRNQWNWVQVSGRISSGV
                     ERKIIREQTVIQFTLIFESLRKTDKEGSHVNFIDVELWGKRAELFYTLIGKGMEIIIS
                     GELFQQRWISKDGKKTQKFCISADALAISDQTFKGN"
     gene            2444..>2749
                     /locus_tag="PLY93_07215"
     CDS             2444..>2749
                     /locus_tag="PLY93_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRP69304.1"
                     /translation="MKLDSKIPQGPLETMWRDYKAKLKLVNPANKRKLDVIVVGTGLA
                     GAAAAASLGELGYNVHAFSFHDSPRRAHSIAAQGGINAAKNYHNDGDSVYRLFYDTVK
                     "
     assembly_gap    2750..2759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2760..4382
                     /locus_tag="PLY93_07220"
                     /pseudo
     CDS             2760..4382
                     /locus_tag="PLY93_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
     gene            4388..4897
                     /locus_tag="PLY93_07225"
     CDS             4388..4897
                     /locus_tag="PLY93_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69305.1"
                     /translation="MRQQSRLFDVRSDGTFILNDTLFIDYRQSHLADLTQELLTLKKR
                     LARTVLHSLKDDNRLLGQEKANISNTLLGILRALAALRLKLFDSENISSFADNPKVLI
                     GGDGYIVEGKLSAQILQPSFSIDTWRQAYLEGAIDDFIHLLKNAAVDKVFTLEERVEL
                     TQALSACRA"
BASE COUNT         1328 a         1266 c         1147 g         1170 t
ORIGIN      
        1 aacacttaga aaaagaaaaa ttgggcgagc gccgagtgca atacaagtta cgcgactggc
       61 ttttttcgcg ccaacgctac tggggggaac ccgtgccgat aagctttact gcggatggta
      121 caatggtcgc cgaaacagaa gagaatttgc cgttggagct acccgaaagc caaaatttcc
      181 aaccctcgcc ttccggtgaa tcaccgctcg cgcatctgac cgactgggtc aacttcaccc
      241 aaaacggagc tgcactccgc cgcgaaacaa acacgatgcc gcaatgggct ggcagctcgt
      301 ggtattattt acgctacgcc gatccacaca acaccgaacg gtttgtatcc aaagaagccg
      361 aagtgtattg gctcaaagat ggagtggatt tatacgtggg tggagcagaa cacgcggtgt
      421 tacatttact ctatgcccgc ttttggcata aggtgctcta cgatttaggg tatgtcacct
      481 caattgaacc tttcaagaaa ttaggccacc aagggatcat cttgggcgaa gacggttcaa
      541 agatgtcaaa gtcgcgcggc aatgtgatta accccgacac cgtagtcgca gaatacggcg
      601 ccgatgcatt ccgcctgtat gaaatgttta tgggacnnnn nnnnnnaaca aatgaagccg
      661 tggtcgtcca aaagcatcga agggatatac cgctttttgg cgcgggtgta tcgtttatac
      721 cttaccgaca atgccatcaa caaaaaatta ctcgctgaac caaaagataa aaaagcaatc
      781 ctcaaaatat tgcatcaaac gattaaaaaa gttacccaag atattgaatc gctttctctc
      841 aatacggcca tttcacagat gatgattttt gtcaacgaag cgtacaaact cgaaagcatt
      901 ggtcgcgatg ccgcatcaac cttccttcaa ctgctccacc cgttcgcgcc acatctcacc
      961 gaagagcttt ggcaacgctt ggggtatagc ggtggattgt tgcaatcggt atggccacaa
     1021 ttcgatgcag cacacctcac cgaagacgaa ttcgaggcgg tcatccaaat caacggtaaa
     1081 atccgcgcac ggcaaaaaat gccgatgggt atctccgacg ccgactttga aaccgccgcc
     1141 cgtgctctcg attcggttaa ggcagcgctc gaaggcaaac ccgcgcgcaa agtgattgtg
     1201 gtgaaaaata agatgattaa ctttgtggtg taatggggat gtctaattag ctgttgacgc
     1261 aaacgtcact cacccccaaa atgctaaaat gcaaaagcaa caacgccgag atgaattcaa
     1321 aattttagcg cgtacttttt ttgaaaaggc atcttccaaa gatttagaga ttttacacca
     1381 aaagattata cagttaatag aacgatacac agaaaaattt ttttcgtcgg gggaaactct
     1441 ccctaaaatt ttaggcatct acaaaccact gcgttacgag ttacccgtca aagaaatgat
     1501 cgcacgcgtg ccgcaactca atgaagtgcc gcttgcgtat cccgagtaca atccgcaaga
     1561 aatgtggttt gtgaaagaag acaacggcac gcattgtgcg ccggatttct tcatcgtgcc
     1621 cggacttttt gtcgaccgca atggctatcg cttggggcgt ggaaaggggt attacgaccg
     1681 ctattttgca gcgcacccta ccccactacc ccggcgggta tttttaggtt acccattcca
     1741 gtttattgac gccgtaccgc acgatgttca tgacgtgacc gtcgttccgc tgtctccagt
     1801 taattccctt tgaaggtttg atccgaaatg gcaagagcat ctgccgagat acaaaacttc
     1861 tgtgtttttt tgccgtcttt ggaaatccaa cgttgttgga aaagttcacc gctaatgatg
     1921 atttccatcc ctttaccgat gagcgtataa aaaagttcgg cgcgcttacc ccaaagttcg
     1981 acgtcgataa aatttacatg cgaaccttct ttatcggttt tacgcaacga ttcaaaaatc
     2041 aacgtgaact gaatgaccgt ctgctcgcgg attatttttc tctccacccc cgacgatata
     2101 cgtcctgaaa cctgtaccca attccactgg ttgcgtgttt tcttttgggt cgtctctggc
     2161 gaactgctct ctacttccat gctttcgttt gtgagctctt cttgtagtgt attcattttt
     2221 gtttcctttt taattttagt aagcaagagg ataaagttcc tcaagcctct atatattaaa
     2281 taccagaaaa cgctcaaatt ttctcacttt tggaaaaaaa agtacaaatt tttgaaaaaa
     2341 aattgtctca gcaagttgcc gtacaggctc ataggtacgc atctcttaca aactataaaa
     2401 tccgtttact gcctaacttc atccgcaatt tcgacaagta aatatgaaac tcgactcgaa
     2461 aatcccccaa gggccactgg aaaccatgtg gcgcgattac aaagctaagc tcaaattggt
     2521 gaatcccgcg aacaaacgca aactcgatgt catcgtcgtg ggtaccggtc ttgccggtgc
     2581 cgccgctgcc gcttctttag gcgaactcgg ttacaacgtc cacgctttct ccttccatga
     2641 cagtccacgc cgcgcgcact cgattgccgc gcagggaggc atcaacgccg ccaagaatta
     2701 ccacaacgac ggcgactcgg tctatcgtct tttttacgac acggtaaaan nnnnnnnnnc
     2761 ggcgactacc gttcgcgcga aggcaacgtg taccgcctcg ccgaagtctc agtgaacatc
     2821 atcgaccaag cggtggcgca aggcgtaccg ttcgcacgcg aatacggcgg cctcctcgac
     2881 aaccgttctt tcggcggtgc gcaggtatcg cgtacttttt atgcaaaagg ccaaaccggc
     2941 caacaacttc ttttaggcgc gtactccgcg ctcaaccgcc aaatccgggc acggtaaaaa
     3001 tgcacacccg ccacgaaatg ctcgacttgg tggtggtcga cggccacgcg aaaggtatta
     3061 tcactcgcaa cttagtcacc ggtgaaatta gccgccacgc tgcacacgcg gttatattgg
     3121 cgaccggcgg ttatggcaac gtgtacttcc tctccacaaa tgcaaagcac tcgaatgcca
     3181 cagcaacgtg gcgtgcacac aaacgcggtg cgtactttgc caacccttgc tatacccaaa
     3241 tccaccccac ttgcataccg acgagcggcg actatcaatc gaaactcacg ctcatgagcg
     3301 agtcgttgcg taacgatgga cgcgtttggg tgcccaaaga caaagcgacg gcagaaaaat
     3361 tacaacgcgg cgagatcacc gcgctcgaca tccccgatgc agatcgcgat tattatctgg
     3421 aacgccgtta tccttcgttc ggcaacttgg tgccgcgtga cgtggcatct cgtgccgcga
     3481 aagaacgctg tgaccacggc ttcggcgtga acgctaccgg tctttcggtg tatcttgatt
     3541 tcaaatcggc gattgaacgc ttgggcgaac acaccatttc tgagcgctac ggcaaccttt
     3601 ttgaaatgta cgaaaaaatc acgggtgaaa atccatacaa aatgccgatg aagatttacc
     3661 ctgccgtcca ctacaccatg ggtggacttt gggtagatta taaccttatg tctactatcc
     3721 ccggtcttta tgtcggtggc gaagcgaact tctccgacca cggcgcaaat cggttgggag
     3781 cgtcggcgct aatgcaaggt ttagcggatg gatactttgt ccttccttcc acgatcggcg
     3841 actacttggc gactgtcggc tttgaccgtc catcgaccga gggcaaggaa tttgtcgagg
     3901 ctgaacaagg cgtcaaagac caaacagcga aatttttaaa caccaaaggt aataaaactg
     3961 tggatgaaat ccaccgccaa ctcggccacg tcatgtggca ctacgtgggc atggggcgta
     4021 ccgaagcggg gctcactaaa gctatcggtg aaatccagca gattcgggaa gacttccaca
     4081 agaacgtaaa agttcctggt tctgccgaca acatcaacga atcgctggag aaagcggggc
     4141 gcgtacaaga ctttttagaa ttgggcgagc tcatggcgcg cgatgcgctg caccgcaaag
     4201 aatcatgcgg tggccacttc cgcgaggagt accaaaccga agaaggcgaa gccaagcgcg
     4261 acgacgccaa ctttgcgtac gtgggtgcgt gggaatacaa aggcgcaggc aaggaagaag
     4321 aactgcacaa agaagagctg aagttcgaca ctgttcattt gacgcagcgt agttataaat
     4381 aggagtcatg cgtcagcaaa gtcgtttatt cgatgtacgt agcgatggaa catttatact
     4441 caacgacacg ttgtttatcg attaccgtca gtcgcattta gctgacctaa ctcaagaact
     4501 tcttactctc aaaaaacgtc ttgcccgaac ggtgttgcat tcgctaaaag atgacaaccg
     4561 cctgctcggg caagaaaaag caaatatctc aaacacactg ctcggtatac tccgcgcgtt
     4621 ggccgcgctg cgcttaaaac tcttcgattc agaaaatatt tcttcttttg ccgataaccc
     4681 aaaagtctta atcggcggcg atggctatat cgtcgaaggc aagttgagcg cacaaatcct
     4741 tcagccttcg ttttccatcg atacttggcg acaggcgtat ttagaaggag cgatcgacga
     4801 tttcattcat ttgcttaaaa acgctgccgt agacaaagtt tttactttag aggaaagggt
     4861 tgaattaact caggcgctat cagcctgtcg ggcttaacaa gagtcttgcc cgccatggca
     4921 gatttctacc c
//
LOCUS       DAOMPD010000176         4923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7193_length_4923_cov_2.985826, whole genome shotgun sequence.
ACCESSION   DAOMPD010000176 DAOMPD010000000
VERSION     DAOMPD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7193_length_4923_cov_2.985826"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLY93_07230"
     CDS             <1..487
                     /locus_tag="PLY93_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69306.1"
                     /translation="PRPAQAAGVPAAMNTRMPTDLPEIPDSDELIAAEYVLGLLDATA
                     RRQISECLQILERHAIDEIELFVAALARLFSEALRFHYYKDSGIEFSEIGKIIGARPI
                     SGYALQKSAERWSILVQKYTPDGIRRIMEALVRADRTLKATSDAAQKQTLLTSFYLML
                     E"
     gene            complement(491..>772)
                     /locus_tag="PLY93_07235"
     CDS             complement(491..>772)
                     /locus_tag="PLY93_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69307.1"
                     /translation="SELLVCSRETRVLKAMSAFWNIEGHKDASGEVLLLPYLEFCEYL
                     KRTYGITVEDANQSLLKLRNQNLLHIRKDVSGKQYIAFSPKVFSYFHIT"
     assembly_gap    773..782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<783..1134)
                     /locus_tag="PLY93_07240"
     CDS             complement(<783..1134)
                     /locus_tag="PLY93_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP69308.1"
                     /translation="MSVDLVKKFGATYKAGQTIFKEGDHAEEMFIIHQGQVNISKRAK
                     NAEQILATLRDGDFFGEMALFTDSARSATATVMNDSVILRIDKKSFDFMVNNNSTFAT
                     NMIRKLCERLRNADN"
     gene            complement(1131..1355)
                     /locus_tag="PLY93_07245"
     CDS             complement(1131..1355)
                     /locus_tag="PLY93_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HRP69309.1"
                     /translation="MENNVLIDIEKGKGEVIRVEVGEFKGKKLLHIRTWYTNEAGELA
                     PSKKGVALSLEQFEKFKSVLTAAEEGLKQP"
     gene            complement(1366..2358)
                     /locus_tag="PLY93_07250"
     CDS             complement(1366..2358)
                     /locus_tag="PLY93_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69310.1"
                     /translation="MKIRFRTAFTLSLAVLSILTAAVMAWFIFRLEKIIAAKLEPNVV
                     MKNFSVSLLNQAFVAEGVSVMADAASPCAGKTLFEVNRATGRFTWRERKIYFIQLDKP
                     TIYDEAFNINCLLPHSKKPGFRLGEELDATGLKVIVRDAQVTLRFVKPIKVNGLFTLK
                     YRETGEPVFLGEALRIENEQGWLSSNRIELLFEKEKGSVGLREGVIKAEVDIPHIEDI
                     PQMSTKKIAVKTGRGNLKLTANIDNYRWVIFSKVDLFKLKLSGKPIYETMFLKLTPQN
                     LWPMVEDKPNFFTVAFKTTGSWKGLPRRYAADFKRAVVKKVKANLKKKIPALPF"
     gene            complement(2367..2699)
                     /locus_tag="PLY93_07255"
     CDS             complement(2367..2699)
                     /locus_tag="PLY93_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRP69311.1"
                     /translation="MSECLFCKIVEKKIEADLVYEDNDVVAFHDISPVAPTHILVIPK
                     VHIENVLGIDGALAQKMLLVIQQIVKMEKIEKGFRLVTNTGEHGGQTVYHLHWHILSG
                     RHMGWPPG"
     gene            complement(2692..4194)
                     /locus_tag="PLY93_07260"
     CDS             complement(2692..4194)
                     /locus_tag="PLY93_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69312.1"
                     /translation="MIYKKYFFIFFLGSFVACRSPQARNEKSENLSTIEGSQDLVEEP
                     KEANTPTDTPLHETKGSLKKDPPQIKKTKKEPKGRFNFSGDEFIKESGSNPFEKKNDT
                     VMRIRGRAKVSTKNLRMVSPQIEIYGSDGRYAYAKGPVELVDTKNNTRITANEALFLR
                     SENKAILRGDTRLQTRINKKKKDIKKELITLTANEMERRFDTATSLARGNVIVKTEDG
                     VLYANEVEFFETQNLVRSSSSPRIFTTTDLILADAIEWNITEKKTEFYKNVKGFFNRQ
                     RSDIKRKDNDSVETTARADNATLFQDENSTFKQRLILRNRIIIERGEFKAFADEAEIF
                     GAAADLIKAKNKIRIINHDEKSESFGDFFEWHKKSGYTSLSARPHFRTRTILYDDKGA
                     ETVEIQSATATRATTDSQPQARGDVRILQREKDKATTSTSANMGGEWAEINPDEKIIR
                     LYGRPYIEGEMGRIAARNIILYYEERRYEMLGIEPGLVEKQMNRSEGEHE"
     gene            complement(4184..>4923)
                     /locus_tag="PLY93_07265"
     CDS             complement(4184..>4923)
                     /locus_tag="PLY93_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP69313.1"
                     /translation="IAHNTLKKEFRGIRPDTTRVLLIEGAPQLLGAFPGSLGESAKKT
                     LTKLGVEVLTNATVKNVAYDGVQISSGTNGVGEFIPAGNVFWAAGNTAATLLKTLGTS
                     LDRAGRAIVESDLSIPGYPEISVIGDAAHTIDYNTGIPLPGVAPVAIQQGQYLARKIK
                     AELSEKAIGAFRYWDKGSMATIGRGRAVAKIGKAHFTGFIAWLMWSFIHILYLIGFRN
                     RFAVMTLWMYGYITNSRTVRLITADDL"
BASE COUNT         1264 a         1220 c          999 g         1430 t
ORIGIN      
        1 gccgcggcct gctcaagctg cgggagtgcc tgcagcgatg aacacgcgga tgcccaccga
       61 cctgcccgaa atccccgaca gcgacgagct gatcgccgcc gaatacgtgc tcgggctgct
      121 cgacgccacc gcgcgccgcc agatcagcga atgtttacaa attttagaaa ggcatgccat
      181 cgacgaaatt gaactttttg tcgcagcgtt agcgcgcctt ttcagcgaag cgctacgatt
      241 tcattactac aaagattctg gcattgaatt ttctgaaatt ggcaagatta tcggcgctcg
      301 ccccattagc ggttacgcgc tacaaaaaag cgccgagcgt tggtcgatcc tcgtacaaaa
      361 atacacgccc gatggcatcc gtcgtattat ggaagcgttg gtgcgcgccg accgcacatt
      421 aaaagcgacg agcgacgccg cgcaaaaaca aacgctattg accagttttt atttaatgct
      481 cgagtaaaaa ttatgttata tggaaataac taaagacttt aggcgagaac gcgatatact
      541 gcttacccga cacgtctttg cggatatgca aaagattttg attacgcaac tttaagaggc
      601 tttggttcgc atcctcgacc gtaataccat acgttctttt aagatattca caaaattcta
      661 aataaggcag caataatact tcacccgatg cgtccttgtg tccttcaata ttccaaaatg
      721 cagacatcgc tttcaacact cgtgtttcac gactacagac aaggagttcg ctnnnnnnnn
      781 nngattgtct gcgttccgca ggcgctcgca taattttcga atcatgttag tggcgaaagt
      841 agagttgtta ttcaccataa agtcgaacga ttttttgtcg atacgcaaaa taacgctatc
      901 gttcatcact gtcgccgtcg ccgatcgcgc agaatcggtg aaaagcgcca tttcgccaaa
      961 aaaatcgcca tcgcgtaacg tcgccaaaat ttgctcggcg ttttttgcgc gcttcgagat
     1021 gttcacctgt ccttggtgga taatgaacat ttcctcggcg tgatcgcctt ctttgaaaat
     1081 ggtttgtccc gctttgtaag tcgcaccaaa tttttttacg agatcaacgc tcatggctgt
     1141 ttcaatccct cttctgcagc agtaaggacg ctcttaaatt tttcaaattg ctccaaactc
     1201 aacgcaacgc cttttttact aggcgcaagt tctcccgctt cgtttgtgta ccaagtgcgg
     1261 atgtgtaaca atttctttcc cttgaactcg cccacttcga cgcggatcac ttcgcccttg
     1321 cccttttcaa tatctatcaa cacattattt tccattttcc ctcgcttaaa acggcaaagc
     1381 cggaattttt ttctttaagt ttgctttcac cttcttaacc accgcacgct taaaatcggc
     1441 agcgtatcgg cgcggcaagc ctttccacga gccggtcgtt ttgaaagcta cggtgaaaaa
     1501 attcggctta tcctcgacca tcggccacaa attctgcggc gttagtttga gaaacattgt
     1561 ctcgtaaata ggtttccctg aaagttttaa tttaaataaa tctactttgg aaaaaataac
     1621 ccaacggtag ttgtcgatat tagcggtcaa tttaaggttt cctcttcccg ttttcaccgc
     1681 tatctttttt gtgctcatct gagggatatc ttcaatatgg gggatatcca cctctgcttt
     1741 gataacgcct tcgcgtagcc caacacttcc tttctctttt tcaaaaagaa gctctatacg
     1801 attcgacgac aaccatcctt gctcgttttc aatacgcaaa gcttccccca gaaaaacagg
     1861 ctcccccgtc tcgcggtatt ttagcgtaaa aaggccgttc acttttatcg gtttcacaaa
     1921 acgtaaagtc acttgcgcat ctctaacaat aaccttgaga ccagtggcgt cgagctcttc
     1981 gcctaaacga aaaccaggtt ttttagaatg gggtaaaaga cagttgatgt taaacgcctc
     2041 atcgtaaata gtgggtttgt ccaactgaat aaaataaatt tttctttcgc gccatgtaaa
     2101 gcgcccggta gccctattaa cttcaaagag agttttgccc gcgcaaggtg aggcggcatc
     2161 ggccataacc gaaacacctt cggcgacaaa cgcttggttg aggagcgaca ccgaaaaatt
     2221 tttcatcaca acattgggtt caagttttgc cgcgataatt ttctcgagtc taaaaatgaa
     2281 ccaagccatc accgccgcag ttaaaatgga gagtactgcc aatgaaagag taaaagcagt
     2341 gcggaatcga attttcacga aattgattag ccgggcggcc agcccatgtg acggcctgat
     2401 agaatgtgcc agtgcaaatg ataaacggtt tgaccaccgt gctcgccggt attcgtcacc
     2461 aagcgaaatc ccttttcaat tttttccatt tttacaattt gttgaattac caacagcatt
     2521 ttctgcgcta aagcgccgtc gatacctaaa acattttcga tatgtacttt gggtatgaca
     2581 agaatatgcg tcggcgcaac cggacttata tcatgaaacg cgacaacgtc gttatcttca
     2641 tagactaaat ctgcttcaat ttttttttct acaattttac aaaacaaaca ctcactcatg
     2701 ttcaccctcg cttcgattca tttgcttttc tacaagtccc ggctcgatac cgagcatctc
     2761 gtagcgccgc tcttcgtagt aaagaataat attacgtgcg gcaattcgac ccatttctcc
     2821 ttcaatataa ggtctaccat aaagtcgtat aattttttca tctggattga tttctgccca
     2881 ctcccccccc atgttggcgc tggtcgacgt tgtagctttg tctttttctc gttgcaaaat
     2941 tcgcacatcg ccgcgcgctt gcggttgcga atctgttgtc gcgcgcgtcg ccgtcgccga
     3001 ttgtatctcg accgtctccg ctcctttatc gtcatagaga attgtacgag tgcgaaaatg
     3061 agggcgtgcc gacagcgaag tgtatcccga ttttttatgc cattcaaaaa aatcaccaaa
     3121 cgattcactt ttttcgtcgt gattgataat ccttatttta ttcttcgctt taattaaatc
     3181 cgccgccgcg ccaaaaattt ccgcttcatc ggcaaacgct ttgaattcgc cgcgttcgat
     3241 gattatacga ttgcgcaata taagacgttg tttgaaagtc gagttctcat cttgaaaaag
     3301 ggttgcgtta tcggcacgag cggtcgtctc aacggaatca ttatcttttc tctttatatc
     3361 acttctttgt cgattaaaaa agccctttac atttttataa aactctgttt ttttttcagt
     3421 tatgttccat tcgatggcat cggcgagtat taaatcggtt gttgtaaaaa tacgcggcga
     3481 cgacgaagag cgcacgagat tctgtgtttc aaaaaactcc acttcgttgg cgtaaagtac
     3541 accgtcttcg gtctttacga taacatttcc ccgagcgaga gaagttgccg tatcgaaacg
     3601 acgctccatt tcgtttgccg tcaacgttat caattccttt tttatatctt tctttttctt
     3661 atttatccgc gtttgaagac gagtgtcgcc gcgcaaaatt gctttattct cgctccgtaa
     3721 aaaaagcgct tcgtttgcag tgatgcgcgt attgtttttt gtatccacaa gctcgacagg
     3781 ccctttcgca tacgcatatc tgccatccga accgtaaatt tcaatttgcg gcgataccat
     3841 acgcaaattt tttgtactta cttttgcgcg accgcgaatg cgcatcacgg tatcgttttt
     3901 tttctcaaag ggattagagc cactttcttt gataaattca tcgccgctaa aattaaaacg
     3961 acctttgggt tcctttttag tctttttaat ttgaggcgga tcttttttta acgaaccttt
     4021 cgtttcatga agaggcgtat cggtgggagt atttgcctct ttaggttctt caactaaatc
     4081 ttgagagcct tctatcgtgg acaggttttc cgatttttca ttacgcgctt gaggcgaacg
     4141 gcaagcaaca aaggaaccaa gaaagaaaat aaaaaaatat tttttataaa tcatcggcgg
     4201 taatcaaccg aacggtgcga ctgtttgtga tataaccata catccacagc gtcatcacgg
     4261 caaaacgatt acggaagcca atcaaataaa ggatgtggat aaacgaccac ataagccatg
     4321 caataaaacc ggtaaaatgc gctttgccaa ttttagcaac cgcacgtcct cgaccaattg
     4381 tcgccatcga acctttatcc cagtaacgaa acgcaccgat agctttctct gaaagttcag
     4441 ctttaatctt tcgcgcaagg tattgccctt gctggattgc caccggcgcc actccgggaa
     4501 gcggtatgcc tgtgttgtaa tcgatggtat gcgcagcgtc accaatgact gaaatttcgg
     4561 gataccctgg gatagacaaa tcactttcga caatcgcacg ccccgcacga tctaaagatg
     4621 tgcccaacgt tttcaaaagc gtcgcagcag tattccctgc cgcccagaaa acattacccg
     4681 cgggaataaa ttcgcccacg ccatttgtgc cgcttgaaat ttgcacaccg tcataagcga
     4741 catttttgac tgtggcgttt gtcagtacct cgacaccgag ttttgtaagc gttttcttcg
     4801 cgctctcgcc caacgatccc ggaaacgctc ccaaaagctg tggcgcacct tcgatgagta
     4861 gtactcgcgt tgtatcgggg cggatacctc taaattcctt tttgagagtg ttgtgtgcaa
     4921 tct
//
LOCUS       DAOMPD010000177         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7202_length_4918_cov_2.555007, whole genome shotgun sequence.
ACCESSION   DAOMPD010000177 DAOMPD010000000
VERSION     DAOMPD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7202_length_4918_cov_2.555007"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLY93_07270"
     CDS             <1..871
                     /locus_tag="PLY93_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP69314.1"
                     /translation="FVNYLKRRVEEKSIVLLGYRVHYLHFPCAKPKATLVCFHGWLDN
                     AASFAPLANALADYEVYAWDFPGHGKSAHRHPGERYHFIDLVPFIDAALISIPAKNKI
                     LVGHSMGAGGAAFYAGSVGESFSKLILLEGGVPFTAPEEKTAAALGESVRFFRKSWST
                     IQSPFYKNKDDMVKIRMRINALSEKAARVLVDRAVVKTPQGYKWRADFRLRAPTLVRL
                     TNAQVMNLAQSIKAPTLIVLGEKGMKELQVLAKHSKKLIRHAVIKTLPGGHHLHMEAP
                     DELVSLMRKFIEE"
     gene            868..1695
                     /gene="panC"
                     /locus_tag="PLY93_07275"
     CDS             868..1695
                     /gene="panC"
                     /locus_tag="PLY93_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRP69315.1"
                     /translation="MKIIKTIREMRAFRPQCTVGFLPTMGYLHEGHLSLVRLAKKEND
                     VVVVSIFVNPAQFNDANDLEKYPTDIERDIQLLERENVDVLFLPTRNEVYPNGIPALS
                     LRYERMMQQLCGAFRPNHFEGVLLIVHNLLLWVAPTRAYFGLKDYQQYLLIKKMSGDL
                     SLAVEIVGAPLVREKDGLAMSSRNVRLTEKGRTEALKISKALFAAKEDVLSGKLSFAQ
                     IKDGLWKVLEPLQIEYATLCDADTLEEIVEKRCQSILIAVAAYVDGVRLIDNALILR"
     gene            complement(1753..2574)
                     /locus_tag="PLY93_07280"
     CDS             complement(1753..2574)
                     /locus_tag="PLY93_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP69316.1"
                     /translation="MNHPIPSGVYLVTGASKGIGRAIASLLAAKGAQLILLARDKKAL
                     ENTARELREKYNTKSVSVFSCDLGDKTQILHTAKEIRKKYKALSGMIHVAGYARPGYF
                     HELDLDDFEKSMRIDYLGAVYLTHALHSLVNDDGLIAFTSSVAGYMGVFGYTAYAGPK
                     FALVGFAETLRQEMLQRKIQISVLCPPDTETPGYTEENKTKPFETQALSKNAKLMPPE
                     KVAQKFIQGIAKKKFIVTCNFESAMLYRLHGLWPSLIFRIMKSMIQKAQKKLPKN"
     gene            2561..3886
                     /locus_tag="PLY93_07285"
     CDS             2561..3886
                     /locus_tag="PLY93_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HRP69317.1"
                     /translation="MGWFIRRSFFNFNTAKTEISNMEVTLAKPIPGYKEVDPFESANP
                     ENSYYGILNLLYYLKAKYVRVHFRYSGYVYIAHCESIAQDEVLLVAHGFQETQERRAV
                     IQFEAYNRYYMARVLVLRTNENGIFIQFPQELLYLQRRKHIRLRFDDLFMRFTILYSP
                     IGHTRLDEKNLESRFPFVMQEVSREDVSLQTMYRMVAAEIENISSDFELVLFSSKSEE
                     ELSLVEKIVRDEGKTFYIEDTMRVLSYTSPYENRHLINLSTLYEELAQKEGEAKALKY
                     IEEIRKSDARAFIVSYILSPISLFNERIGYVRVETNPFDKKYINPFQAADLHRLMELF
                     SYAMSKVRIRTSHFNPNSVETRVVNISLSGLLLEFSDAAIFEYLKKNRRIKMLIPVMG
                     EDLEFYGEIVRFNERSGFYYLGVLFFKSKPGDMIRLERFIHENMQFQFL"
     gene            3892..4356
                     /locus_tag="PLY93_07290"
     CDS             3892..4356
                     /locus_tag="PLY93_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69318.1"
                     /translation="MLLKEVAKIFSEKNIPYAVVGGYAVNLYGFVRSTLDLDILLPLQ
                     LEVFQEVERCMAALGLICRVPIDAHKLFQSLDYYRHEKNMVVWSFYSEKKPWLIIDFL
                     LTDDLRDFEIETKILDGISIAVITLEGLITLKKKSARRQDLEDIRALEQNKK"
     gene            4359..4577
                     /locus_tag="PLY93_07295"
     CDS             4359..4577
                     /locus_tag="PLY93_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69319.1"
                     /translation="MNRPVQLYDAEKQKLTQDMPIEEILAFLEDVQRMTPLETYLTIH
                     REYVQKFGQSTIIDATSKNPFHSANDNG"
     gene            4564..>4918
                     /locus_tag="PLY93_07300"
     CDS             4564..>4918
                     /locus_tag="PLY93_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRP69320.1"
                     /translation="MTMAESPQTNLNFSDRRACNSVGESDNNATIVVCGWVYRYRDQG
                     GVLFVDLRDRSGVVQVVFDMAADAELLKKADSLRSEDVIYVTGKVRLRDKNAINPKLK
                     TGTVEILATSLTLLSK"
BASE COUNT         1314 a         1024 c         1188 g         1392 t
ORIGIN      
        1 gttcgtaaat tacttgaaac gccgcgttga agaaaaatca atcgtgttat tgggctaccg
       61 cgtccattat ctacacttcc cttgcgcaaa acccaaagcg acgttggtct gcttccatgg
      121 ctggctcgat aacgcggcct cgtttgcgcc gttagcgaat gcattggcgg attatgaagt
      181 ctatgcatgg gattttccag ggcatgggaa aagtgcccac cgccatccgg gggaacgcta
      241 ccattttatc gatttagtcc catttatcga tgcggcgctc atcagtatcc ctgcaaagaa
      301 taaaatttta gtcggccatt cgatgggggc agggggagcg gcgttctatg ccggttcggt
      361 gggagagagt ttttctaaat taattctttt agaaggcggc gttcctttta ccgctcccga
      421 ggagaaaacg gcagcggctt taggcgagag cgttcgtttt tttcgtaaat cgtggagcac
      481 aatacaatcg cctttttaca aaaataaaga tgatatggtg aaaatccgga tgcggatcaa
      541 cgcgttgtcc gaaaaggcgg cgcgtgtgtt ggtggatcgt gctgtcgtta aaacccccca
      601 aggctataaa tggcgcgccg actttcggtt gcgtgcgccc acgttggtgc gattgacaaa
      661 tgcacaggtg atgaatttag cgcaaagtat taaagcgccc actctcattg tgttgggaga
      721 gaaggggatg aaagagctgc aagtattggc aaagcactca aagaagttaa tccggcacgc
      781 cgtcatcaaa acgttgccag gcgggcatca tttacatatg gaagcgcccg atgaactcgt
      841 gtctctcatg cgaaaattta tcgaagaatg aaaatcatta aaacgattcg agagatgcgc
      901 gcctttcgtc cgcaatgtac ggtgggcttt ttaccgacga tgggctattt gcacgaaggc
      961 catttgtcgc tggtgcgtct ggcaaaaaaa gaaaacgatg ttgtcgtcgt ttccattttt
     1021 gtgaacccag cgcagtttaa tgacgccaac gatttggaga aataccccac cgacatcgag
     1081 cgcgacatcc aacttttaga gcgcgaaaat gtcgatgttc tttttttgcc gacgcggaac
     1141 gaagtttatc ccaacggtat ccccgctctg agtttgcgct acgagcgcat gatgcagcaa
     1201 ttatgtggcg cgtttcgccc aaatcatttt gaaggcgtgc tcctcattgt gcataatctt
     1261 ttattgtggg ttgctcccac gcgcgcgtat tttggactca aagactatca gcagtatttg
     1321 ctcattaaaa aaatgagtgg cgacttgtcg ctcgctgtgg agattgtggg agcgccgttg
     1381 gtgcgtgaaa aagacggttt ggcaatgtcg tcgcgcaacg tgcgcttgac ggagaaagga
     1441 cgcacagagg cgctaaaaat ttcaaaagcg cttttcgccg ctaaggaaga tgtcctttcg
     1501 ggtaagctat ccttcgctca aataaaagat gggctttgga aagttttaga accgttgcaa
     1561 attgaatacg ccacgctttg cgatgcagac accctggagg aaattgtaga aaagcgctgc
     1621 cagtcaatcc ttatcgcagt ggcggcgtat gtggatggtg tgcggctcat cgataatgca
     1681 ttgattttgc gctaatacta cgcaacttta tctataaccg ttcgagttgg cgcacaggct
     1741 ctaagctctt tgtcagtttt ttgggagttt tttttgtgct ttttgaatca tgcttttcat
     1801 aatacgaaaa atgagcgaag gccatagacc atgcagacgg tagagcatcg ccgattcaaa
     1861 attacaagtg acgataaatt ttttttttgc aatcccttgg atgaattttt gcgccacctt
     1921 ttccggtggc atgagttttg cattttttga aagcgcttgt gtttcaaaag gctttgtttt
     1981 attctcttcg gtatagcccg gtgtctctgt gtcaggggga cagagaacgc tgatttgtat
     2041 tttacgttga agcatttctt ggcggagcgt ttcggcaaaa ccaacgagcg caaatttggg
     2101 gcctgcgtac gcggtgtatc caaacacccc catataaccc gccaccgacg aagtgaacgc
     2161 gatgagtccg tcgtcattaa ctaacgaatg cagtgcatgt gttaaataaa cggcgcctaa
     2221 gtagtcaatg cgcatcgatt tttcaaagtc atccagatcg agttcgtgaa aatagcctgg
     2281 gcgtgcgtaa ccggctacgt gaatcatccc cgacaacgcc ttgtattttt tgcggatttc
     2341 tttagccgtg tgtagaattt gtgttttatc gcccaagtcg catgagaaga cgctcacgct
     2401 tttggtatta tatttttcgc gcagttctct tgccgtattc tcgagtgctt ttttatcgcg
     2461 tgcgagtaaa ataagctgtg cgcctttcgc agcaaggagt gaggcaatcg cgcgtccaat
     2521 gccttttgag gcaccggtga caagatatac gccgctgggt atgggatggt tcatacgccg
     2581 ctcatttttc aacttcaaca cggcgaaaac cgaaataagt aatatggagg taactctcgc
     2641 aaagccgatt ccggggtata aagaagtcga tccgtttgaa agcgctaacc ctgaaaattc
     2701 gtattacggt atactcaatc ttctttatta cttgaaagca aaatacgtac gcgtgcattt
     2761 tcggtattcg ggatacgtgt atattgcgca ctgtgaaagt attgcacaag acgaggtgct
     2821 gcttgttgcg cacggttttc aagaaacaca agaacggcgt gcagtgattc agtttgaagc
     2881 gtataaccgt tattatatgg cgcgggtttt agttttgcgc acgaacgaga acgggatttt
     2941 tatccagttt ccccaagagt tgttgtattt gcaacggcgt aagcacatcc gcttacgttt
     3001 cgacgattta tttatgcgct ttactattct ttactcgccg attggtcata cgcgtctcga
     3061 cgaaaaaaat ctagaaagcc gctttccctt tgtcatgcag gaagtttcgc gcgaagacgt
     3121 ttcgctacaa accatgtacc gcatggttgc cgctgaaatt gaaaatatca gtagcgattt
     3181 tgaactcgtt cttttttctt cgaagagcga agaagaactt tcgcttgttg aaaaaattgt
     3241 gcgcgatgaa ggtaaaacat tttacatcga agacaccatg cgagtgctct cgtatacatc
     3301 cccctatgaa aacagacatc tcatcaattt gagtactctt tatgaagagt tggcgcaaaa
     3361 agaaggcgaa gcaaaagcgt taaagtatat cgaagagatt cgtaaaagcg acgcgcgcgc
     3421 ttttatcgtt tcgtatatct tgagtcctat ttcacttttt aacgagcgca taggttatgt
     3481 gcgcgtcgaa accaatccgt tcgataaaaa atatataaac ccctttcaag cggccgactt
     3541 acaccgactc atggaacttt tttcgtatgc gatgagcaaa gtacgtatcc gtacttcgca
     3601 ttttaatcct aattcagtgg agacgcgggt ggtgaatatt tctctttcgg ggcttttgct
     3661 cgaattttca gatgcggcaa tttttgaata cctcaaaaaa aatcggcgga ttaaaatgct
     3721 gatccccgtg atgggagaag atttagaatt ttacggtgaa attgtacgct ttaatgagcg
     3781 tagcggtttt tattatctcg gcgtgctctt ttttaagagc aagcccggcg atatgatccg
     3841 cctagagcgg tttattcacg aaaatatgca attccaattc ttgtaaaagt tatgctctta
     3901 aaagaagttg caaaaatttt ttcagaaaaa aatattcctt atgctgtggt gggagggtat
     3961 gccgtcaatt tatacggttt tgtgcgatcg acacttgact tagatatttt attgccgttg
     4021 caattagaag tttttcaaga agtcgagcgg tgtatggcgg ctcttggatt aatttgtcgt
     4081 gtacctatcg atgcgcataa actttttcag agtttggatt actaccgcca cgaaaaaaat
     4141 atggtggtgt ggagttttta ttcagagaaa aaaccatggt tgattattga ttttttattg
     4201 accgacgatc ttcgtgattt tgaaatagaa acaaaaattt tggacggtat aagtattgcc
     4261 gtaattacct tggaaggact cattacatta aaaaagaaat ctgcccgtcg gcaagattta
     4321 gaagacatcc gtgcgttaga gcaaaacaaa aaataaaaat gaatcgacct gtgcaattat
     4381 acgatgcgga gaaacaaaag ctcacccaag atatgcccat cgaagaaatt ttggctttct
     4441 tagaagatgt gcagcggatg acgccactgg aaacctattt aacaatacac agagaatatg
     4501 tgcagaaatt tgggcagtct acaataattg acgccacgtc taaaaatcct tttcactctg
     4561 caaatgacaa tggctgaaag ccctcaaaca aatttgaatt ttagcgaccg ccgggcgtgt
     4621 aactctgtcg gtgaaagcga caacaatgcc acgattgtcg tttgcggttg ggtgtaccgc
     4681 taccgcgatc agggaggagt gctttttgtc gatttgcgcg accgcagtgg cgtggtgcaa
     4741 gtcgtttttg atatggctgc cgatgccgag ttactcaaaa aagccgatag cctgcgtagc
     4801 gaagatgtga tttatgtgac agggaaagtg cggttgcgcg ataaaaatgc aatcaacccc
     4861 aaactaaaga ccggcacagt tgaaatctta gccacctctc ttacattatt gagtaaag
//
LOCUS       DAOMPD010000178         4917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7205_length_4917_cov_2.683875, whole genome shotgun sequence.
ACCESSION   DAOMPD010000178 DAOMPD010000000
VERSION     DAOMPD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7205_length_4917_cov_2.683875"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLY93_07305"
     CDS             complement(<1..658)
                     /locus_tag="PLY93_07305"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP69321.1"
                     /translation="MAKAQSTKKITPRFREDILTIFRASDGIERQVHFWGPNKPKVIF
                     LAIHGGMAHAGDWMTPALYFKKKDWATAAFDLHGHTGQVRVDIPSFNIFIDDVELFLQ
                     WIKIRYPKTPIFVLSHSMGALIATHFAIKKRPNGDAQINGYIMSSPYFGNAIKVSKLL
                     IMLSKVMAALLPKMKVPLPSLTENLTHDSTITARHRKDEKELLRATESSLRFGNALLR
                     A"
     gene            complement(737..1528)
                     /locus_tag="PLY93_07310"
     CDS             complement(737..1528)
                     /locus_tag="PLY93_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69322.1"
                     /translation="MIRCQALFIFSLLPLGALFANMRAPIHVNRAGITLYAPKANLVL
                     LGEKVIFDCPESFVGKHDYAKFIARVCRAKVQYSIRAPQATEVLLQFIYSGSKEVSWI
                     YQGKKMVTVARTFTVAKSDLCNYCPDDFNTLQKVQQEIQFIKGESVLEIDYEQQLSYE
                     ERNLGYFSTSTWLQSFTYELWPIAEWQWGENPKAELVFSVKARDSFLGFGYKDDALQC
                     QTIEGETKTLIPLTFSPVKSGKRTAVATVALRRAPLQLQCSYIAK"
     gene            1553..2191
                     /locus_tag="PLY93_07315"
     CDS             1553..2191
                     /locus_tag="PLY93_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRP69323.1"
                     /translation="MMANLFKNAPSSLYLIMDGEACAARGIALDDFLVGALEGGAWLI
                     QYRHKNIDTELYEKNLQRFLPLCVQHHTHLIVNDHAALAEKFNLPLHIGQEDFLPPNL
                     SVPYGRSIHNMDELSTVLSAQPAASYIALGSMFTSPTKPEVKPMRAIAPMVLEEIEVP
                     LVLIGGISLDNASRLPRSERVFYAIITDTFRFGSTRADIRRYVESWHRSASA"
     gene            complement(2215..2940)
                     /locus_tag="PLY93_07320"
     CDS             complement(2215..2940)
                     /locus_tag="PLY93_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69324.1"
                     /translation="MHNAPSKVKNAAFDTFLEKQFAIIKEEIDSQIKKKEVGKKVLFK
                     SGISPAAGKKSRWQYGLAALVAVGVAVPLLFELNQTQLQEKEALATPRIVIPDAVPAP
                     KNAKPKKEITRVAPTPSHAQKRSKKKEIEQEYNAPTPRLRAAPSFADSVAVDAKKAEV
                     EASRAEGGGIQRKADAQQPSAAPFQVTPQNMADIEKREMEKLWREYKRKPSVFMKDAA
                     RVQQLKVLLRRYDTTGRIKEIDP"
     gene            complement(2933..3505)
                     /locus_tag="PLY93_07325"
     CDS             complement(2933..3505)
                     /locus_tag="PLY93_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRP69325.1"
                     /translation="MVLRYFSYCMSLADANFFERLMPYRERLYRLALAQLQNTHDAED
                     AVHEALIKASHNLQTFRNESNEYTWVVRILINHCYDVQRKAQRRKKREAGGDKLEQQT
                     TLIVDTRETPERNIELSETSQHLMDAVNDLSKNYKPLILMRYFENRSYEDIAVALEIQ
                     LGTVKSRMNQAKILLKQKLIQRGVTEDFLA"
     gene            3557..4057
                     /locus_tag="PLY93_07330"
     CDS             3557..4057
                     /locus_tag="PLY93_07330"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRP69326.1"
                     /translation="MKKSDPPLYYTYIKSPIDKLCLISNGGALTHLLMEKHRHAPHIE
                     SHWIEKENLPIFNEATRQLNAYFSGKLKTFNLPIDPSGTTFQKRVWRALMRIPYGETL
                     SYGQLAHRVGNPNASRAVGLANGRNPISIIIPCHRVIGASGSLTGYGGGVERKRFLLE
                     LEGFKL"
     gene            4108..4428
                     /locus_tag="PLY93_07335"
     CDS             4108..4428
                     /locus_tag="PLY93_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRP69327.1"
                     /translation="MVEQISVFELKKKLDNKASITLVDVRQVNELDICKLPEAKHIPL
                     HILMHRLDELDRSSEIIMMCHGGGRSQRAAEFLVSQGFTNVANLVGGIDAWAAQIDSN
                     MARY"
     gene            4430..>4917
                     /locus_tag="PLY93_07340"
     CDS             4430..>4917
                     /locus_tag="PLY93_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific 2-thiouridylase"
                     /protein_id="HRP69328.1"
                     /translation="MARILCAMSGGVDSAVSAFLLKEEGHDVVGANMRFWEYPSDLSC
                     STETKKKSRHTSCCSPEDMADAERSARGMDIPFYALKMEGDFRENVIDPFIKDYEEGL
                     TPNPCVHCNTFIKFGEFYDKATALGFDKIATGHYAAIVRLDSGRYAVAPAADAKKDQS
                     YYL"
BASE COUNT         1194 a         1161 c         1147 g         1415 t
ORIGIN      
        1 gtgcgcgtaa aagggcattg ccaaaacgta acgacgactc ggttgcacgc aataattctt
       61 tctcatcctt gcggtggcgc gcggtaattg ttgagtcgtg cgtgagattc tctgtcaacg
      121 agggtagagg gactttcatt ttgggcaaaa gcgccgccat tactttagaa agcataatta
      181 acagtttgga aactttaatg gcgttaccaa aatacggcga cgacatgatg tagccattga
      241 tctgtgcgtc gccattcggt cttttcttga ttgcaaagtg tgtcgcgata agcgccccca
      301 tcgaatgtga aagaacaaaa ataggggttt tgggataacg tattttaatc cattgtaaaa
      361 aaagctcaac atcatcgata aatatattaa acgaagggat gtcgactcga acttgacctg
      421 tgtgaccgtg taaatcaaac gcagcggttg cccagtcttt ctttttgaag tagagagcgg
      481 gtgtcatcca gtcacccgca tgcgccatac cgccgtggat tgctaagaaa atgactttcg
      541 gtttattggg tccccagaaa tgcacttgcc tttcaatgcc atcgctcgct cgaaaaatgg
      601 taagtatatc ttcgcgaaag cgcggtgtta tttttttggt cgattgtgct tttgccatgg
      661 cgccctcttt ttggggagat tttttagtac agccctattc attaattatc gcataatttg
      721 atttattcgt gtagcgttat tttgcaatat acgagcattg aagttgtaga ggagcacgcc
      781 ttaacgcaac cgttgctacc gctgtacgct taccgctttt tacgggtgag aacgtcaacg
      841 gtattaatgt ttttgtctct ccttcaatcg tttggcattg aagcgcgtcg tctttatagc
      901 caaaacctaa aaagctatcg cgtgctttca cagaaaatac cagttcggct tttggatttt
      961 ctccccactg ccattcggca atcggccata gttcgtacgt gaaactttgt aaccatgtac
     1021 tcgtgctgaa atagccaagg ttgcgttctt cgtaagaaag ttgttgttcg taatcgattt
     1081 caagtacgct ttcccccttg ataaattgaa tttcttgctg cactttttgt aatgtattga
     1141 agtcgtcggg acagtaattg cacaagtcac tttttgccac tgtaaatgtt cttgctacag
     1201 ttaccatttt tttgccttgg taaatccaac ttacttcttt tgaaccagaa tagataaatt
     1261 gtaaaagtac ttcggttgct tggggcgcac ggatgctata ttgtactttg gcgcgacaga
     1321 cacgcgcgat aaattttgca taatcgtgtt tacccacaaa cgattcaggg caatcaaata
     1381 tgactttctc gcctaaaagt acgaggtttg ctttgggtgc gtacaatgtt attcctgctc
     1441 gattgacgtg aatcggagcg cgcatgttgg caaaaagagc gccaagcggt aaaagactaa
     1501 aaataaaaag tgcttggcat cgaatcatag gcgatcaaac ttcgtactat acatgatggc
     1561 aaatcttttt aaaaatgccc ccagctcgct ttatttgatt atggatggtg aagcctgcgc
     1621 ggcgcgcggt attgcgctcg atgacttttt ggtgggagca ttggaagggg gagcatggct
     1681 catccaatac cggcacaaaa acatcgacac cgagctttat gaaaaaaatt tacaacgctt
     1741 tctgcccttg tgtgtgcaac atcatacgca tctcatcgtg aacgatcacg cagcgctcgc
     1801 tgaaaaattc aacttgccgt tacatatcgg tcaggaggat tttttacctc ccaacctgtc
     1861 ggtaccgtat gggcgttcta tccacaatat ggacgaattg tcgacagtgt taagcgcaca
     1921 acccgccgcc agttacattg cactgggatc gatgttcacg agtcccacta agccagaggt
     1981 aaaaccgatg cgtgcaattg caccaatggt gctcgaggaa attgaggtgc cactcgttct
     2041 cattggaggt atttctctcg ataacgcatc gcgtttgccg cgctcggaac gagtgtttta
     2101 tgccatcatc accgatacgt ttcgcttcgg ttcaacacgt gcagatatac gccgctatgt
     2161 agagagttgg caccgatctg cgagtgcata attttgtaga tactcttgag cttcttatgg
     2221 atctatctct ttgatacgcc ctgtggtgtc gtaccgccgc aacaacactt ttaactgctg
     2281 aacacgcgcg gcgtctttca taaaaacact cggttttctt ttgtactccc gccacaattt
     2341 ttccatttca cgtttttcaa tatccgccat attttgaggc gttacttgga atggtgccgc
     2401 gctcggttgt tgagcatcgg ctttgcgttg gatgcctccc ccttccgccc gagatgcttc
     2461 tacttctgcc ttttttgcgt cgactgcaac agaatcggca aacgaaggcg ctgcacgcaa
     2521 acggggcgta ggcgcgttgt actcctgttc tatttctttc ttttttgaac gtttttgtgc
     2581 atgcgatggc gtgggcgcta ctcgcgtaat ttctttttta ggctttgcat ttttgggcgc
     2641 cggtaccgcg tcggggataa ctatcctcgg cgttgcaaga gcttcttttt cttgtagttg
     2701 ggtttggttg agttcaaaaa ggaggggcac tgcgacgccc accgccacaa gcgcggcaag
     2761 accatattgc caccgcgatt ttttaccggc ggcaggcgaa atcccacttt tgaaaagaac
     2821 ttttttaccc acttcttttt tcttaatctg tgaatcgatt tcttctttaa ttatagcgaa
     2881 ctgcttttct aaaaacgtat cgaatgcagc gttttttact ttagaaggcg cgttatgcaa
     2941 ggaaatcctc cgtgacgccg cgttgaataa gtttttgctt gagcaatatt tttgcttggt
     3001 tcatacgcga cttcaccgta cccagttgga tttcgagcgc aacggcaata tcttcgtagc
     3061 ttctgttttc aaaataacgc atgaggatta acggtttgta atttttgctc aaatcgttca
     3121 ccgcatccat tagatgctgc gaggtctcag aaagttcgat atttctttcg ggcgtttcgc
     3181 gtgtgtcgac gatgagcgtc gtctgttgtt caagcttatc gcctcccgct tcccgttttt
     3241 ttcgccgttg cgctttgcgt tgcacgtcgt aacagtggtt gattaaaatg cgcaccaccc
     3301 aagtatactc gttcgattcg ttgcggaagg tttgcagatt atgcgacgct ttgatgagcg
     3361 cctcgtgtac cgcatcttcg gcgtcgtgcg tattttgtag ttgcgccaac gctaatcgat
     3421 aaagccgttc gcgatagggc atgagccgct caaaaaaatt cgcatcggca agactcatgc
     3481 agtacgaaaa atagcgcaat accatggacg gtgagtattc accggtagaa agcgtcgtgt
     3541 caaacgaaaa gtaattatga aaaaaagtga ccctcctctt tattacactt acatcaaaag
     3601 ccccatcgac aaactttgcc ttatctccaa cggcggcgca ctcacgcatc tattgatgga
     3661 aaagcaccgg catgcgccac acatcgaaag ccattggatt gaaaaggaga atttacctat
     3721 tttcaacgaa gcgacacgac aactcaacgc gtatttcagc ggtaagctta aaacgtttaa
     3781 tttaccgatc gaccccagcg gtaccacctt ccaaaaaaga gtgtggcgcg cgctcatgcg
     3841 cattccctac ggtgaaacgc tttcttacgg tcaactcgcg caccgcgtgg gaaaccccaa
     3901 cgcgtcacgt gcggtgggcc tcgccaacgg tcgtaacccc atttccataa ttattccctg
     3961 ccatcgcgtc atcggcgcct cgggatcgct caccggctat ggtggtggcg tcgaacgtaa
     4021 gcgttttttg ttggaattgg aagggtttaa actttagata cttctaaaaa tctggcaaaa
     4081 gaaatatctt ctttatatat tctctttgtg gtagaacaga tttccgtttt tgagttgaag
     4141 aagaagttag acaacaaagc aagtattact ctcgtcgacg tgcggcaagt gaacgaactc
     4201 gacatttgca aacttcccga ggcaaagcac atccctttac atattttaat gcaccgcctc
     4261 gacgagctcg atagatcgtc ggagattatt atgatgtgcc acggtggtgg ccgctcgcag
     4321 cgcgcggcag agtttttggt ttcgcaaggt ttcacaaacg tcgcaaatct cgttggtggc
     4381 atcgacgcct gggcggcgca aatcgactcc aacatggcgc ggtattgata tggcgcggat
     4441 tttgtgcgcc atgtcgggcg gcgtcgattc ggcggtctct gcatttttac tcaaagaaga
     4501 agggcacgat gtcgtcggcg cgaatatgcg cttttgggaa tacccctctg acctttcgtg
     4561 ttccaccgaa actaaaaaga aatcacgcca cacttcgtgt tgctcacccg aagacatggc
     4621 cgacgccgaa cgctctgcgc gcggtatgga cattcctttc tatgcgctca aaatggaagg
     4681 cgattttcgt gagaatgtta ttgatccttt tatcaaagac tacgaagagg gattaacacc
     4741 caacccttgc gtccactgca atacgtttat taaatttggc gagttctacg acaaagccac
     4801 tgcgttgggt tttgataaaa ttgccaccgg ccactacgcc gctatcgttc ggttagatag
     4861 tggtcgttac gcggtcgctc cggcggcgga cgccaaaaag gaccaaagct attatct
//
LOCUS       DAOMPD010000179         4917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7206_length_4917_cov_2.683464, whole genome shotgun sequence.
ACCESSION   DAOMPD010000179 DAOMPD010000000
VERSION     DAOMPD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7206_length_4917_cov_2.683464"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLY93_07345"
     CDS             <1..615
                     /locus_tag="PLY93_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP69329.1"
                     /translation="HFKELYGEIIAVPESRIVQPADGETIRVGELELEFIYTRGHANH
                     HFVIFDKASKSIFTGDSFGVAYPILQGGKFPFLFPSTSPTDFDAAEARISYDKIIKSG
                     ATTAYPTHYGAWQDIALGYKMLNAAIDEIEKMFNAIYQSTMDDKAAQSFAREQIERYF
                     TRELAERGILLNAEGKTVLEMDIDLNSQGIAFAALRARKKVQGA"
     gene            612..1424
                     /locus_tag="PLY93_07350"
     CDS             612..1424
                     /locus_tag="PLY93_07350"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRP69330.1"
                     /translation="MNQQMLEICEVAPRDGLQSASKILTVEERVLFVESLYARGFKKI
                     EVGSLVNPTRVPTMAHSDAVFIALVKAHGENKNNYSLLVPNEKGFDRAIQIGCKSVAF
                     FTATSETFNKKNINRTVAESIAELHLLIERARAQGMRVRLYISTVFDCAYEGIKNPEV
                     DLQQFISLFSLVDEISLGDTTGKATEAQVKSLMAHAILKPYREKIWWHFHDTYRLAGA
                     NVRACLAAGFRRFDASAGGIGGCPFSPGAKGNIATEKVVALAEELGIPHQFI"
     gene            complement(1412..1633)
                     /locus_tag="PLY93_07355"
     CDS             complement(1412..1633)
                     /locus_tag="PLY93_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69331.1"
                     /translation="MKRRQQQCGELLALGKVARAETFLCGHIVLTFDSFKVGFSRDDF
                     YDFANTIAMASMNLACRDKERAEWGLYMN"
     gene            1869..3887
                     /locus_tag="PLY93_07360"
     CDS             1869..3887
                     /locus_tag="PLY93_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69332.1"
                     /translation="MRFLYFTLTPLCIFLFSVIHSDAPEIIRFNFTSSELKILPGNSG
                     EKNKGYGCNGVLKDEDQIGDFAIASEFHQNACYCDENFSNKNYTVELGSVASADGSQT
                     FVAVRVHPPASTAAKFLASKHLGFVRANEMMGVYELDDGLLSKAFKTSKKGGLTGGFL
                     RIENFYLSNLDRPRVYVFIPLSAKFSPYSPMGGGVEAARIFNDGNFVLEKYRIPVKGN
                     IFNGNRDFNNGPGSYGGVYGHNRTYFMSPQADGIAIVWQDQESDEVFATFVKNNFTAY
                     RQVKLARERNFLLAAATVDGSGNVYHLMIERSRDSSTFATRRVTAFKANAQGRANKRM
                     TLSGGPNGLNIAEFNPDKSCSMAYSNGKLGVLISRQMHRSPDGLNHQGAIAFVLDAET
                     LHLDKNFGQTSGHSFGNVTTVDSDGNFLAVDLGDNYPRGVHLHRFNATEKVSRLFFSV
                     KTRHGTESRSNAGKGYPIYKEISGGGKTYYQWSNDTNTYAEIGGVDEMQNGIALVFAG
                     EPNSSGYALDNSRVGAILNDARNVGFIFVKKNFTEADAVLSKGINEKGGFYTFGGTFA
                     PQENKGVVWLTRYRDVENQNATRVKTARLDGNRLLVLHEIWNRRGYGSSVLSILQTDK
                     GKLEKSINIGALMPLGWRDDLIVKDGFVYAVTGNGVDDKLEIIQIKIK"
     gene            complement(3892..4290)
                     /locus_tag="PLY93_07365"
     CDS             complement(3892..4290)
                     /locus_tag="PLY93_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69333.1"
                     /translation="MFQRITFKSGIYTVFFFLTGAALSADSFCFEHTQNKTVSRMTFN
                     SDYNVISDGVYRVVVNGKTRRSAKFHGTKSGTVYLITFTDKIPYTKPKGDSSIVWTRD
                     GNTLHVPMAGRNKKTKKSAVYNAVFHSCRK"
     gene            complement(4296..>4917)
                     /locus_tag="PLY93_07370"
     CDS             complement(4296..>4917)
                     /locus_tag="PLY93_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRP69334.1"
                     /translation="FIYFIGFWSKPNGVQTDYKRFRGYRHNLVDWVYADSDATVESLY
                     GKSGERYATSLSSFEPKGKFWEREKGEPESWRRESGPEGLGGDNHYAGMEILRFIRKG
                     FIANGIDSASSAPKILKPYMSTWAVPTYVNAVAPFLEIAHTRSKRDYTRMAKHRHIYA
                     EAIAVAAYSLLYGLKTPRDTSLPKGAAVNWSRYENLASGNYFKQVK"
BASE COUNT         1301 a         1120 c         1218 g         1278 t
ORIGIN      
        1 catttcaaag aactgtacgg tgaaattatt gccgtgcccg aaagtcgcat cgttcaaccc
       61 gccgacggtg aaacgatacg cgtgggggag cttgagctgg aatttattta tacacgcggg
      121 catgcaaacc atcattttgt tattttcgac aaagcctcga agagtatttt tacaggggac
      181 tcgtttggtg tagcgtatcc tattttgcag ggtggaaaat tcccttttct tttcccttcg
      241 acaagcccca ccgatttcga cgctgccgag gcacgcataa gctacgacaa aattataaag
      301 agcggtgcaa ccacggctta ccctacgcat tacggtgctt ggcaagacat tgcgttgggg
      361 tataagatgc tgaatgcagc gattgacgaa attgaaaaaa tgtttaatgc catttatcaa
      421 agtaccatgg acgataaagc ggcacaatct tttgcgcgcg aacaaattga aagatacttt
      481 acgcgcgaac tcgctgagcg aggcattcta ctcaacgcag agggaaaaac cgttttagaa
      541 atggatatcg acttgaattc acagggaatt gcgtttgccg ccttgcgcgc acgtaaaaaa
      601 gtgcaaggag cgtgaaccaa caaatgctcg aaatttgtga agtcgcgccg cgtgatggct
      661 tgcagtccgc atctaaaatt ttgacagtgg aagaacgcgt gctctttgta gaaagcctct
      721 acgcgcgcgg ctttaagaaa attgaagtgg ggtcgttggt gaatcctacg cgtgtaccaa
      781 cgatggctca ttcggatgct gtattcatcg cgcttgtaaa ggcgcacggg gaaaacaaaa
      841 ataattattc tcttttggtg cctaatgaaa aagggtttga tcgtgcgatc caaattggtt
      901 gtaagtcggt ggcttttttt actgcaactt ctgaaacttt taacaaaaaa aatatcaacc
      961 gcacggtggc cgagtcaatc gccgagctgc atctactcat cgaaagagcg cgcgcacagg
     1021 gcatgcgagt acgcctttat atttcaacgg tgttcgactg cgcatacgaa ggcatcaaaa
     1081 atcccgaagt ggatctacaa caatttattt ctttattttc acttgtcgat gaaatttcac
     1141 tgggcgacac cacgggtaaa gcaaccgaag cgcaggtgaa aagtttaatg gcgcacgcaa
     1201 ttctgaaacc ctaccgtgaa aaaatctggt ggcacttcca cgatacctac cgactcgctg
     1261 gcgcaaatgt gcgtgcatgc cttgccgctg gtttccgccg cttcgatgcg tcggcgggag
     1321 gaattggtgg ctgtccgttt tcgcccgggg caaagggaaa catcgccaca gaaaaagtgg
     1381 tggcactcgc cgaagagcta ggtataccgc atcagttcat ataaagtccc cactcggcgc
     1441 gttctttatc gcgacaagcg aggttcatgg aagccatagc aatcgtgttg gcgaaatcgt
     1501 aaaaatcgtc acgcgaaaaa ccaaccttaa aagaatcaaa agtaagtacg atatggccgc
     1561 agagaaaagt ctcggcgcgt gcaacttttc ccaatgctaa aagttcacca cactgttgct
     1621 gtcttcgttt catagtatat tttcaagagt atagttatcc cgtcaatgca aatgataata
     1681 attctcagta tcataaatac gaaaatttct tttttgagtt tttggtgctt gggtatagtt
     1741 atcccttgtg agtggaaata ccaaagaaaa gagattttat gtggtgaatc accacagagc
     1801 ctgtgcgcca actccgggaa cttatttttg tcttgaattt tcaataggat taaaacttgt
     1861 atccgaatat gcgattttta tattttactc taaccccatt atgtattttt cttttttcgg
     1921 ttattcattc cgatgcgccg gaaatcatcc gtttcaattt tacttccagt gagctaaaaa
     1981 ttttgccggg taactcgggc gaaaaaaata agggctatgg ttgcaatggc gttctcaaag
     2041 atgaagacca aatcggtgat tttgccatcg cttccgaatt tcatcaaaat gcgtgctact
     2101 gcgatgaaaa ttttagtaat aaaaattaca cagtggagtt gggtagcgtg gcatccgccg
     2161 atggctcgca aacatttgtc gcggtgcggg tgcatccccc tgcgtccacc gcagcaaaat
     2221 ttcttgcttc aaagcattta ggatttgtga gagccaacga gatgatggga gtctatgagt
     2281 tggacgacgg gctgctctcg aaagcattca agacatccaa aaaaggcgga ttgaccggcg
     2341 gatttttgcg aatcgaaaat ttctatttga gtaatttaga tcgcccccgc gtgtatgtgt
     2401 tcataccgct ttcggccaag tttagcccgt actcgccaat gggcggcggc gttgaagcgg
     2461 cgcgtatttt taacgacggt aattttgttt tagagaaata ccgcataccg gtgaagggca
     2521 atatttttaa cggcaaccgc gattttaata acggccctgg gagttatggc ggtgtgtacg
     2581 gccacaaccg cacgtatttt atgagtcccc aagccgacgg tattgccatc gtttggcaag
     2641 atcaagagag cgacgaagtt tttgccactt ttgtcaaaaa taattttact gcgtatcgtc
     2701 aagttaagct ggcgcgcgaa agaaatttct tgctcgccgc agccaccgtc gatggaagcg
     2761 ggaatgtgta ccacctcatg atcgaacgca gccgcgatag cagtacattt gcaacgcgcc
     2821 gcgtcaccgc gttcaaggcg aatgcgcaag gcagggcgaa taaacgtatg acactgagcg
     2881 gcgggcccaa tggacttaat attgccgagt ttaaccccga taaaagttgc tcgatggcat
     2941 acagcaacgg taagttgggt gttttgatta gccggcaaat gcaccgctct cccgatggat
     3001 taaaccacca aggcgcgatt gctttcgtgc tcgatgccga gacgctccac ctcgacaaaa
     3061 attttggcca aacatcaggc cattcgtttg gtaatgtcac caccgtcgat agcgatggta
     3121 attttcttgc cgtcgattta ggggataact atccccgtgg ggtgcattta caccggttta
     3181 atgcaacaga aaaagttagc cgtctttttt ttagtgtaaa gacacggcac ggaaccgaat
     3241 cgcgctctaa tgccggcaaa ggatacccca tatataaaga gataagcggt ggaggcaaaa
     3301 cgtattacca atggtcgaac gacaccaaca cgtatgccga aattggcggc gtcgacgaaa
     3361 tgcaaaacgg tatcgcgctt gtctttgcgg gcgaacccaa ctcttcgggg tatgcgctcg
     3421 acaactcacg cgtgggcgct attctaaacg acgcccgtaa tgtgggattt atttttgtaa
     3481 aaaagaactt cactgaggcg gatgcagtgc tctccaaagg catcaacgag aaaggtggat
     3541 tctatacttt cggcggtacg tttgcgcctc aagaaaacaa aggcgttgtg tggctgactc
     3601 ggtaccgcga cgttgaaaat caaaatgcaa cgcgcgtcaa aactgcccgc ttagatggca
     3661 atcgactgct cgtgttgcac gaaatttgga accgccgtgg ttatgggtct tcggtgttgt
     3721 cgatactgca aaccgacaaa ggtaagctcg aaaaaagcat aaatatcggc gctctcatgc
     3781 cgttaggctg gcgcgacgac ttaatcgtga aagacgggtt tgtatacgcc gtcaccggca
     3841 acggcgtgga cgacaaactc gaaataatcc aaatcaaaat aaaatgattc cctacttgcg
     3901 gcatgagtga aacacagcgt tgtacaccgc cgattttttc gttttcttgt tacgccccgc
     3961 cattggcaca tggagggtat tcccgtcgcg cgtccataca atgcttgaat cgcctttggg
     4021 tttggtgtaa ggtattttgt cggtgaaagt gatgagatac actgtgcccg actttgtacc
     4081 gtggaatttt gcactgcggc gtgtcttgcc gtttacaacg acccgataca caccgtcact
     4141 gatgacattg tagtcgctgt tgaaggtcat ccgactgact gttttgtttt gggtgtgctc
     4201 aaagcaaaag gaatcggcag acaacgccgc gcctgtcaaa aaaaagaaga cagtgtaaat
     4261 cccacttttg aacgtaattc tctggaacat tgtttctatt tcacctgctt gaagtagttg
     4321 ccacttgcta aattttcata gcgtgaccaa ttcaccgcag cgcctttggg gaggctcgtg
     4381 tcgcgtggcg ttttcaaacc gtagagtaac gaatacgcag cgacagcgat agcttcggcg
     4441 taaatatggc ggtgtttggc catacgtgta tagtcgcgtt ttgagcgcgt gtgtgcaatt
     4501 tccaagaatg gtgcgactgc gttgacgtac gttggcaccg cccacgttga catgtatggt
     4561 ttcaaaattt ttggcgcgct tgatgcggag tcgatgccgt tggcgataaa acctttgcgg
     4621 atgaatcgca aaatttccat accagcgtaa tggttgtcgc cacccaagcc ttcagggccg
     4681 ctttcgcgcc gccacgattc gggttcgcct ttttcgcgct cccaaaattt tcctttgggt
     4741 tcaaatgatg acaaactggt ggcgtaacgt tcgccacttt tgccatagag cgattcgacg
     4801 gtcgcgtccg aatcagcata gacccagtcg acaagattgt ggcggtaacc gcgaaagcgt
     4861 ttgtagtcgg tttgcacgcc gttgggttta gaccaaaaac cgataaaata tataaac
//
LOCUS       DAOMPD010000180         4839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7337_length_4839_cov_2.158027, whole genome shotgun sequence.
ACCESSION   DAOMPD010000180 DAOMPD010000000
VERSION     DAOMPD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7337_length_4839_cov_2.158027"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLY93_07375"
     CDS             complement(<1..391)
                     /locus_tag="PLY93_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRP69335.1"
                     /translation="MNTKIFFVFLLILVTTFSIHADENYNKALAAYEQKNFDTCLTVL
                     KTVIRENETNYDAHILAAYCHVGRKTYSDAIYHLREVAENSLQHAQTVRTDIVNLYFA
                     MGKYKTARNVGYKYLEAFEDENTKAPLD"
     gene            complement(595..2868)
                     /locus_tag="PLY93_07380"
     CDS             complement(595..2868)
                     /locus_tag="PLY93_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP69336.1"
                     /translation="MPTLLKPKPLFLFYVWLSQRISFGGVLILLSLPLIPLVNLYAYF
                     FLHGIVPGDLKQIYHPESLVFPYAFTGALSLFFVLGGALHRLGIPSVLPAVRRLEPSV
                     RGFTKSYSKDELRSILGSLSNIPNQLTYLLIGIFLLQNSIVFGYGFYTRGNVTDIKLF
                     YIVFIVALIMLSGLINAITDFMCAFMRIRVKRSLGILGESINDEAHAKSETSSLFFIF
                     FVGSVSTISLITFLYFNHDSTFWPAIGVGTILFIICAVLIAIYIYTFRFSLNQISDAM
                     RDVAMGGRGLLPLFSNGRELTQLAINFDTATQEIHSIRNYLNDLVDEKTQKITDAFNE
                     LKHLKSRQDGDYFLTANLIDSLIEIRPESETVLVEALAKQFKTFEFMEHTREIGGDMN
                     SAYALHLGDEKYTLVVNADAMGKSLQGAGGVLVLGTALRSLLERTYITEELRKLSPER
                     WLKNAFLELHRLFASFNGSMMVSMVCLLVHDRSGYTVMINAEHPRGILLRAGHSEYIS
                     GHDGLRKLGNTAVKGRLWVDTFMFQEGDVIILGSDGRDDVIVGYDEHKQPVINQDDDN
                     FLTIVRMEAGDLTRIYQRLKKTSEIVDDISLVRVEYQSLTIQHNNEAVSAQWHEYHRT
                     VKSTEKLIILREILHLDPNDTKAWRALLYLHLKAENLIDAASVSETLVQLTPQRDTLL
                     FVAALLSYRIGDYAKALHYADRLTLRNWHNENYLVLLGKIYEAKQDTKGMREAGMLLV
                     ELEPLHEYGKKLLEMAR"
     assembly_gap    2982..3024
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3025..>3240)
                     /locus_tag="PLY93_07385"
     CDS             complement(<3025..>3240)
                     /locus_tag="PLY93_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572250.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRP69337.1"
                     /translation="VAARHVGFDVIYSGIRLTPEEIALAAVEENADLLALSILSGSHI
                     ELIEQLMQHLKKHHADKEIPVAVGGIIP"
     assembly_gap    3241..3270
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3271..>4839)
                     /locus_tag="PLY93_07390"
     CDS             complement(<3271..>4839)
                     /locus_tag="PLY93_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRP69338.1"
                     /translation="NARATNELYRNGLERGQTGLSIAFDLAAQCGDDSDHPMARPEVG
                     KVGVPINSLVDFRILFDQIPLEKMNTSMTINGSAMWLLSLYIALAEERGVDQKLLQGT
                     TQNDIIKEYLARGTYIFPPEASVRLIVDMYEYCVRHIPKWNPSNICSYHLQEAGATPV
                     QELSFALVTAIAILDAIKERKCFSDEEFEQAVGRISFFVNSGMRFIEEMCKMRAFGAM
                     WETITKERYGVKNEKFSRFRYGVQVNSLGLTEEQPENNAWRILIEALGVTLSRDARCR
                     ALQLPAWNEALSLPRPWDQQWALRMQQVMAYETDLLEYPDIFNGSPVIESKVASLIKE
                     AHAEIEKVLLEGGAIEAIKSGYMKSQLVKSQAERMSRIMTGDLKIIGRNVFTDGLPSP
                     LLGGDDAGIFKVDTHAADETLKALAEVRATRDSAAVEKALAELANDAKANKNLMPASI
                     QCAKVLVTTGEWANTLRKVFGEYRPLTGVEGQRLSLAESRVAAVREKINAFKAKHGHR
                     PRLVLGKPGLDGHS"
BASE COUNT         1271 a         1091 c         1148 g         1256 t
ORIGIN      
        1 gatcgagagg ggctttcgta ttttcgtcct cgaacgcttc gaggtattta tagccgacgt
       61 tacgcgccgt tttgtacttg cccatggcaa aatacaaatt tacaatgtcg gtgcgtacgg
      121 tctgggcgtg ttggagggaa ttctcggcga cttcgcgcaa gtggtaaatg gcgtcgctgt
      181 aagttttacg gccaacgtgg cagtaggcgg caagaatgtg tgcgtcataa ttcgtttcat
      241 tttcgcggat gactgttttt agtacggtta agcaggtatc gaaatttttt tgttcatacg
      301 ctgccagcgc cttattataa ttttcatcgg cgtggataga gaatgtggta acaagaatca
      361 ataagaatac aaagaaaatt ttagtgttca tatagtggca acgcggttca taaagacaca
      421 gcgaaaagaa gtaaatgagt tacgacgcac acagtctacg actactttcc gcgcactaaa
      481 gcgtgatcca ttaaatttca cgaaatttta atttttagag agggaagaag ggcttggtgc
      541 gcgagttgcg catgggttcg taagtcttca acagaataaa aaatacaccg tgctttaacg
      601 agccatttcg agtaattttt ttccgtactc gtggagcggt tcgagctcaa caagaagcat
      661 gcctgcttct ctcattcctt tagtgtcttg cttcgcctcg taaatttttc ctaagagcac
      721 taagtagttc tcgttgtgcc agttgcgtag agttaaacga tcggcgtagt ggagcgcctt
      781 tgcgtagtcg cctatacgat acgaaagcaa tgcggcaaca aaaagcaaag tgtcgcgttg
      841 aggagtcaat tgaactaagg tttcactcac cgaggcggca tcgataagat tttcggcttt
      901 caaatgcaag tataataacg cacgccatgc tttagtgtcg tttggatcaa gatgtagaat
      961 ttcgcggagg attatcaatt tttcagttga tttcaccgtg cggtggtatt catgccattg
     1021 cgctgaaact gcttcgttat tatgttgaat cgtaagcgat tggtactcga cacgcacgag
     1081 agaaatatcg tccacgattt cactcgtctt tttgagtcgt tgataaatcc gcgttaaatc
     1141 acccgcttcc atccgtacga tcgtcaagaa attatcgtcg tcttggttaa tgacgggctg
     1201 cttatgttca tcgtaaccga caattacatc gtcacggccg tcagaaccta aaataatgac
     1261 gtctccttct tggaacatga aggtatccac ccataggcgc cctttcactg cggtgttacc
     1321 caatttgcgt aagccatcat gcccagaaat atattcgctg tgccccgcac gcaacagaat
     1381 accgcgcggg tgttcggcat taatcatcac ggtatagcca gagcggtcgt gtaccagaag
     1441 acaaaccatc gacaccatca tgctaccgtt aaagctcgcg aaaagtcgat ggagttcaag
     1501 gaacgcattc ttaagccatc tttcggggga aagtttgcgt agctcttcgg tgatgtaagt
     1561 tctttcaagg agagagcgca atgcggtacc taaaacaaga actccccctg cgccttgcag
     1621 cgattttccc atagcatcgg catttacgac gagtgtgtat ttttcatcac ccagatgaag
     1681 cgcgtacgct gagttcatat cgccgccaat ttcacgcgta tgttccataa actcaaatgt
     1741 cttaaactgt tttgctaacg cttcgacgag aacggtttcg ctttcaggac gaatttcaat
     1801 caacgaatcg atgaggtttg cagttaaaaa atagtcccca tcttggcgcg acttgagatg
     1861 ctttaactcg ttaaacgcat ccgttatttt ttgtgttttt tcgtcgacca agtcgtttaa
     1921 gtaatttcgt atcgagtgga tttcttgagt tgctgtatca aaattgatgg caagctgagt
     1981 tagttcgcgc ccgttagaga ataagggcaa tagaccgcga cctcccatcg cgacatcgcg
     2041 catcgcatcg ctaatttgat tcaatgaaaa gcggaatgta taaatataaa tagcgattaa
     2101 cactgcgcag atgataaaaa gtatcgttcc tacacctatt gccggccaga aagttgaatc
     2161 gtggttaaag tataaaaagg taatgagaga gattgttgaa actgaaccca cgaaaaaaat
     2221 aaaaaacaaa ctacttgttt ccgatttggc atgcgcttca tcgttgatgg attcgcccag
     2281 aatccccagg ctacgcttga cgcggatacg catgaacgca cacataaaat cagtgattgc
     2341 attgatcaaa cctgagagca taatcaatgc gacgatgaag acgatataaa aaagtttaat
     2401 atcggtaaca ttcccccgtg tatagaaacc ataaccaaac acaatactgt tttgtaataa
     2461 aaaaatccca atgagaaggt aagtgagttg attagggatg ttcgaaagag aacccaagat
     2521 actgcgcagt tcgtcctttg agtaactttt tgtaaacccg cgcaccgaag gctcgagacg
     2581 gcgcactgct ggtagcaccg acggaatccc caagcggtgg agcgcgccac ctaaaacgaa
     2641 aaaaagagag agtgccccag taaaagcgta agggaagacg agcgattcag ggtggtaaat
     2701 ttgttttaag tcaccaggga cgatgccgtg cagaaaaaaa taggcataca aattaacgag
     2761 cggtataaga ggtagcgaca acaatataag aacaccgccg aacgaaatgc gctgcgagag
     2821 ccacacataa aacaaaaaaa ggggcttggg tttaaggaga gtcggcatta atgcacaaac
     2881 ttttcaacaa gtgttaaatt cttctccatg attgcgataa ggtcgtaatc tttaggagtg
     2941 aaaatctgtt gtacgcccat tgcgcgcaac cgttcaaaat cnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnggggat gataccaccc actgccacgg ggatttcttt
     3061 gtcggcgtgg tgctttttaa gatgctgcat caattgctca atgagctcga tgtgactgcc
     3121 cgacaaaata gagagcgcga gtaaatctgc gttttcttcc actgccgcga gtgcaatttc
     3181 ttcgggcgtt aaacggatgc ccgagtagat aacatcgaac cctacatgac gcgctgcgac
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggagtggcc gtcgaggcca ggctttccta
     3301 acactaaacg tggacggtgg ccgtgctttg ccttaaacgc gttgattttc tcgcgtactg
     3361 ccgctacacg cgattcagcg agagataacc tctggccttc aacccctgtg agagggcggt
     3421 actctccgaa aactttacgt aaagtgttag cccattcgcc ggtggtgacc aatactttcg
     3481 cgcattggat tgaagcgggc attaaatttt tgttggcttt tgcgtcgttg gcaagttctg
     3541 ctaacgcctt ttcaacggca gcactgtcgc gcgtcgcacg cacttctgcg agggctttca
     3601 acgtctcgtc ggcagcgtgc gtgtcgactt tgaaaatgcc tgcatcgtcg ccgcctaaaa
     3661 gcggagaggg gagtccatcg gtaaaaacgt tgcgcccaat aattttaagg tcaccggtca
     3721 taatccggct catgcgctcg gcttggcttt tcaccaactg tgatttcatg tagcccgatt
     3781 taatcgcctc gatcgctccc ccttcgagga gaactttttc aatttcagcg tgcgcctctt
     3841 tgatgagcga ggccactttc gattcaatga cgggagaacc gttgaaaata tccgggtatt
     3901 caagcagatc ggtttcatac gccatcactt gttgcatccg caacgcccat tgttgatccc
     3961 atgggcgggg gagcgagagt gcttcgttcc acgcagggag ttggagtgcg cggcagcgtg
     4021 cgtctcgcga cagggttact cccaacgctt caatgagaat acgccatgcg ttattttccg
     4081 gttgttcctc ggtgaggccg agcgaattca cttggacacc ataacggaac cgggaaaatt
     4141 tttcattctt cactccatag cgttctttag tgatggtctc ccacattgcg ccaaacgcac
     4201 gcattttgca catctcttcg ataaaacgca tacccgagtt cacaaaaaaa gaaatacggc
     4261 cgacagcttg ctcaaattct tcgtctgaaa agcatttgcg ttctttaatt gcgtctaaaa
     4321 tagcaattgc ggtcaccagt gcgaacgaca actcttgtac cggtgtcgct cctgcttctt
     4381 ggaggtggta tgaacagatg ttggatggat tccatttggg gatgtggcgc acgcagtatt
     4441 cgtacatatc cacaataaga cgtactgacg cttcaggggg aaaaatatac gtaccacgcg
     4501 cgaggtattc tttaataatg tcgttttgtg ttgtgccctg caacagtttt tgatcgacgc
     4561 cgcgctcttc tgcaagcgcg atgtaaagag ataagagcca cattgccgag ccgttgatgg
     4621 tcatcgatgt gttcattttt tcgagaggga tttgatcaaa gagaatacga aaatccacga
     4681 gcgagttgat aggcactccc accttaccca cctcagggcg tgccattggg tggtcggaat
     4741 catctccaca ctgcgccgca aggtcaaacg caatcgaaag accggtttgg cctcgctcta
     4801 aaccgttacg gtaaagctcg ttggttgcgc gtgcgttag
//
LOCUS       DAOMPD010000181         4753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7512_length_4753_cov_2.913367, whole genome shotgun sequence.
ACCESSION   DAOMPD010000181 DAOMPD010000000
VERSION     DAOMPD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7512_length_4753_cov_2.913367"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="yidC"
                     /locus_tag="PLY93_07395"
     CDS             complement(<1..1383)
                     /gene="yidC"
                     /locus_tag="PLY93_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRP69339.1"
                     /translation="MLSSFRTVVKNHPQKLRRTLAATLLAALFVLPLPAANENKIPEP
                     LQMEVTKRGDAKKTLAIDTQHFSIKFSEQGARIVEFKNRDKAYPRRDGADIVLPGVEI
                     FLSPYLGATGTTQYVNANLAAMMYAAFDIKKEENASAITVTATAPVNLIVDNIKYPVT
                     IVKEFVFLKNSPYFKFSIQLQAQTTVKLQNLMLYALPMIGPVPEKTGSFRDYHHYTEQ
                     GKVEFAYAGSSGGLGCGGATTTEKLSNGPSEFFGTSSRFLILNMQPLFTTRSVSITPE
                     HRDPTTHQLLQTQALHVNMGDIILEKNKTQRFEYIGYMGPKLDDNTYPSDEARKAMPD
                     LAKFHKNYFSKSFDFGITEPIRDILVIALNLFYKLIPNYGVGIILIALLLKLAFFPLN
                     QKQADAMKRMGELQPKIKELNEKYKNNPQEKQKRMMELYKVHKVNPVSGCLPMLIQLP
                     VFIAMYSAFSD"
     gene            1478..3070
                     /locus_tag="PLY93_07400"
     CDS             1478..3070
                     /locus_tag="PLY93_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69340.1"
                     /translation="MKKALKILKKVILGFFALLFVIMIGGYIAIKVVVTKDFIATKIE
                     ENTNARVEIGDISVPIWAAFSGITVDNLKIGNRDAEIKKPMAERKPMQSQVIGFKKFN
                     FQVALGALITSFGKNFELKSLLITEPTAAIVLGANGGNNLQALLIKPKTKEDLAKEAA
                     DAKEKKAEEAKKAAEATASKEPSKPFDIREIPTVIKMGKIGIEGGAFTVNVQKFGQTL
                     KLTNVNVMVRDILIDPKDLEHKNHVGLTAQFVMELANTGSGVKSFKIIYDLQGGLAPF
                     NPKTGGPAESVTVTTGLKKGSYVTGLAVFEKLKNQTENLKKIGVNLNFLNETQTLTKD
                     ALSTIYYNAGVVTFQKPPTLATADFEFALTEKDYINVKTLDHLFRGDLSLAQQHTKKV
                     EADLDKAIEGGITPALAQIPAGPVRDKAKASVTPAKVRASLLAPAMKNGLITFGIESK
                     ASISAPAVRVVRPEFPSLKDIIQAELKKAAPDLKGMVNAEVDKLKDKAKAEANKQIDK
                     AKEEAKQKATNEIKKNLPKLPF"
     gene            3073..3384
                     /locus_tag="PLY93_07405"
     CDS             3073..3384
                     /locus_tag="PLY93_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69341.1"
                     /translation="MKDVVLYVFFCSVYGIIALHFFAKNIPTGSTVTWYALGYHALLF
                     TAVATLARWTVMAFKKRNRLLKGQKIVGVEESVAPPVIEVIKPVVITQEIKKPRLRKR
                     K"
     gene            complement(3389..4006)
                     /locus_tag="PLY93_07410"
     CDS             complement(3389..4006)
                     /locus_tag="PLY93_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRP69342.1"
                     /translation="MAFPAFFDKAPRIKVRDPLSEFLGASDDGIIEYSYADAVRLAGH
                     SCPTVAGSYLMARAALKELYGDAIPERGGIEVIMSEGESEGTTGVIAQVFTLITGAAA
                     RNGFHGIAGNFTRSGLLEYNGKDPQAIAALRRSDTGQTVRVALDVSQVPGSPEMRPLM
                     SRALATNADKATIQAFGKLWQERVERVFEHADDENVILVEKVLRV"
     gene            complement(4011..4274)
                     /locus_tag="PLY93_07415"
     CDS             complement(4011..4274)
                     /locus_tag="PLY93_07415"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRP69343.1"
                     /translation="MAFDLTPEPNVHIFDARGIARRFRHSAIFGALSALQNGEAMRFI
                     NDHDPIPLLGQLNQRFGEHLTVTYKQRDETGVVIDFGISGLPE"
     gene            4404..>4753
                     /locus_tag="PLY93_07420"
     CDS             4404..>4753
                     /locus_tag="PLY93_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69344.1"
                     /translation="MESTQTIKTPPLVALKNTAMEAARNHEQKVKEIKKLSNYRWRMF
                     WSGAWAGLVTVSVFLVSLVSWGTTLSGWLYARLLNFGCMRILGIKIKVEGMENLPTAP
                     SVILINHQSNLDAH"
BASE COUNT         1309 a         1086 c         1158 g         1200 t
ORIGIN      
        1 atcggagaac gccgaataca tcgcaatgaa aaccggtagt tggatcaaca tcggcaaaca
       61 tcccgaaacg gggttgactt tgtggacttt gtaaagctcc atcatgcgct tttgtttttc
      121 ttgcggatta tttttatatt tttcgttgag ttctttaatt ttgggttgta actcccccat
      181 gcgcttcatc gcatcggctt gtttttggtt aagcgggaaa aaagccagtt tgagtagaag
      241 agcgatgagg ataatcccca cgccgtaatt gggtatgagt ttatagaaaa gattaagcgc
      301 tatcacgagg atgtcgcgga tgggttcggt gatgccaaag tcaaacgact tgctaaaata
      361 atttttgtgg aattttgcga ggtcgggcat tgctttgcgc gcttcgtcgc tgggataagt
      421 gttgtcatcg agcttaggcc ccatgtaacc gatgtattca aagcgttggg ttttattttt
      481 ctctaaaata atgtcgccca tgttgacgtg gagcgcctgc gtttgcaaaa gttggtgagt
      541 ggtgggatcg cggtgctcgg gggtgatgct cactgaacgc gttgtaaaca gcggctgcat
      601 attcaaaatt aaaaaacgag aagaggttcc aaaaaattcc gaagggccgt tgcttaactt
      661 ttcagtagtt gtcgctccgc cgcatcccaa gcctcctgac gaacctgcgt acgcaaattc
      721 tactttccct tgctcggtgt agtggtggta gtctcgaaaa ctacctgttt tttctggaac
      781 aggaccaatc atggggagcg cgtagagcat cagattttgt aactttaccg ttgtttgcgc
      841 ttgcaattgg atagaaaact taaagtaagg gctgtttttt agaaaaacaa attctttaac
      901 aatggtgacg ggatatttta tgttgtccac gataagattc accggtgccg tggcagtgac
      961 ggtgattgcc gaagcgttct cttctttttt tatatcaaac gccgcataca tcatcgcggc
     1021 aaggtttgcg ttgacgtatt gcgtggtgcc ggttgcgccc aagtaaggag agagaaaaat
     1081 ttcaacgcct ggcaaaacaa tgtccgcgcc atcgcggcgg ggatatgctt tgtcgcggtt
     1141 tttgaactcg acaatccgtg cgccttgctc tgaaaattta atcgaaaagt gttgagtgtc
     1201 aatcgcgagc gttttttttg catcgccacg cttcgtgact tccatttgta agggttcggg
     1261 gattttgttt tcgttggcag caggtaacgg cagtacaaag agtgcggcga gcagagtcgc
     1321 ggcaagtgtt cggcgtagtt tttgagggtg gttcttgacc acagtacgaa aagaagagag
     1381 catagagggt tatcatttgt cttaaagaca tagggtaaat cacttaaaaa cttgtttttg
     1441 aattgactca gagtcggcgt ctatacatta taaccttatg aagaaagcgc tcaaaatact
     1501 aaaaaaagtc atcctcggtt tttttgcttt gctctttgtc atcatgattg gcggctatat
     1561 cgccatcaag gttgtagtca ctaaagactt catcgcgaca aaaattgaag agaacaccaa
     1621 cgcacgcgtt gaaattggcg acatctctgt cccgatatgg gctgctttct cgggcatcac
     1681 tgtcgataac ttgaagattg gcaaccgcga cgctgaaatc aaaaaaccaa tggctgagcg
     1741 taaacccatg caatcacaag tgattggttt caaaaaattt aatttccaag tggcgttggg
     1801 cgcactcatc acctcgttcg gtaaaaattt tgaacttaaa tctctcctta ttacagaacc
     1861 cacagcggcg attgtattgg gagcaaacgg cggcaataat ttacaagcat tgttgatcaa
     1921 accaaaaaca aaagaagact tagcgaaaga agccgcagac gctaaagaga aaaaggcaga
     1981 agaagccaaa aaagcagcgg aagctaccgc ctctaaagaa ccttcaaaac cgtttgatat
     2041 ccgcgaaata ccaacagtca tcaaaatggg gaaaataggc atcgaaggcg gagcttttac
     2101 ggtgaacgtt caaaaattcg gccaaacgct aaaactcacc aacgtgaacg tgatggtgcg
     2161 cgacattctc atcgatccca aagacctcga acataagaac catgtggggc tcaccgccca
     2221 attcgtgatg gaactggcga acaccggcag cggcgtaaaa tcgttcaaga tcatttatga
     2281 cttacaaggc ggcttggctc cttttaatcc taaaacaggc ggtcctgccg agtcggtcac
     2341 cgtgaccacc ggtcttaaga aaggctcgta cgttaccggt cttgcggtat tcgaaaaact
     2401 taaaaaccaa accgagaacc ttaaaaaaat tggggtgaat ctcaatttct tgaatgaaac
     2461 ccaaactctc actaaagatg cattgagcac aatctattac aacgccggcg tggtgacgtt
     2521 tcaaaaacca cccaccttgg caaccgccga ctttgaattt gcgctcaccg aaaaagacta
     2581 tatcaacgta aagacgctcg accacctttt ccgtggcgat ctttctttag cgcaacaaca
     2641 cacgaaaaaa gttgaagccg atttagacaa agccatcgaa gggggcatca cccctgcgct
     2701 ggcacaaatc cccgcagggc cggtacgcga caaggcaaag gcttcggtaa cgcctgccaa
     2761 agtccgcgct tctcttttag cgccagcaat gaaaaatggt ctcattactt tcgggattga
     2821 atcgaaagcg tctatctctg cgcctgcagt gcgtgtggtg cgccctgaat tccccagcct
     2881 taaagacatc atccaggccg agttgaaaaa agcagcgcca gacctgaaag gtatggtcaa
     2941 tgccgaggtg gataaactta aagacaaggc aaaagcagag gcaaataagc aaatcgacaa
     3001 agccaaagaa gaagcaaaac aaaaagcaac gaacgaaatt aaaaagaatt tgcccaagtt
     3061 gcctttctaa aaatgaaaga cgtagttctc tacgtctttt tttgttcggt gtacggaatt
     3121 attgcactcc atttttttgc taaaaatatt cctacaggaa gtacggtgac gtggtatgcg
     3181 ctgggttacc acgcattgct ttttactgcc gttgcgacgc ttgcccgttg gacggtgatg
     3241 gcgtttaaga aacgcaaccg tttactcaaa ggccaaaaaa ttgtgggtgt cgaagaaagt
     3301 gtcgcaccac cagtgattga ggtcataaag cccgttgtca ttacacagga aataaaaaag
     3361 ccgcgtctgc gaaagcgaaa ataaagtcct aaactctcaa cactttttcc accaaaatca
     3421 cattctcatc gtcggcgtgt tcaaagaccc gctcgacgcg ttcttgccaa agtttgccaa
     3481 acgcttggat ggtggcttta tcggcgttgg tggccagggc gcgactcatg agcgggcgca
     3541 tttcgggcga gcccggcacc tgcgacacgt cgagcgcgac gcgtacggtt tggcctgtgt
     3601 cgctgcggcg caatgcagca atcgcttgcg ggtctttgcc gttgtattcg aggaggccgc
     3661 tgcgtgtaaa attaccagca atgccgtgga aaccgttacg cgctgctgca ccggtgataa
     3721 gggtaaagac ttgcgcgatg acaccagtgg tgccttcgct ttcgccttcg ctcataatca
     3781 cttcgatgcc gccgcgttcg gggattgcat cgccgtagag ttctttgagc gccgcgcgtg
     3841 ccatgaggta cgagccagcg acggtggggc acgaatgccc cgctaagcgt accgcgtcgg
     3901 cataactgta ttcaataatg ccgtcatcag atgctcccaa aaattccgat aatggatcgc
     3961 gcactttgat gcgcggggct ttgtcaaaaa atgcggggaa cgccatacgt ctactcgggt
     4021 aatccgctga tgccgaaatc gatcaccaca ccggtttcgt ctctttgttt ataggtaacg
     4081 gtcaagtgtt cgccaaagcg ttggttgagc tgtcccaaaa gtggaattgg gtcgtgatcg
     4141 ttgatgaagc gcatcgcctc gccgttttgg agcgcgctca atgcgccaaa aatcgccgaa
     4201 tgccgaaaac gacgcgcaat gccgcgtgca tcaaaaatat gcacattggg ttctggtgtt
     4261 aagtcaaatg ccatgagtaa attctcctga agtgcgatta aaacagataa gtttatccat
     4321 tattctcctt gaatctaatc tttactttcg ctttcgacca aaaaaaattg caaccgtaag
     4381 tcactttgag gcatctaata aatatggaaa gtactcaaac aataaaaact ccacctctag
     4441 ttgctctcaa gaacacggcg atggaagccg cacgcaacca tgaacagaaa gtaaaagaaa
     4501 taaaaaaatt aagtaactac cgctggcgta tgttttggtc gggcgcatgg gcggggcttg
     4561 tcaccgtgag cgtcttttta gtgagcttgg taagctgggg caccaccctc agcggctggc
     4621 tttatgcgcg tcttttgaat tttggctgta tgcgtatcct tggcatcaag attaaagtcg
     4681 aaggaatgga gaatttaccc acggcgccat ccgtcatcct gattaaccat caatccaact
     4741 tagacgcgca tta
//
LOCUS       DAOMPD010000182         4680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7679_length_4680_cov_3.268973, whole genome shotgun sequence.
ACCESSION   DAOMPD010000182 DAOMPD010000000
VERSION     DAOMPD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7679_length_4680_cov_3.268973"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLY93_07425"
     CDS             complement(<1..221)
                     /locus_tag="PLY93_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69345.1"
                     /translation="MPIKKTVGCRLSFFIFIATALFASSGQKYLHSLSSNDPELKKMR
                     AEVTQNLRTVADGRVPHVVWRRYKIQKKDT"
     gene            254..1126
                     /gene="prmC"
                     /locus_tag="PLY93_07430"
     CDS             254..1126
                     /gene="prmC"
                     /locus_tag="PLY93_07430"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRP69346.1"
                     /translation="MSNSIPILIDVLRKAENFLAEKKIPSPRLEAQLIFAHFLNLKRI
                     EIFTQSERPLNATELEMLRQALREKITGKPTAYILGQKDFYTRTFKVDSTVLIPRPET
                     EELVEHVLKKIPSTKHIVDLGTGSGCIGVTLAAESGCERITLVDISRGALSIAEENAA
                     RHIDGAKTKIVVIDGDFTQPSFILSEKADVIVTNPPYVLAEEFNSLDETVRSFEPRLA
                     LVADDFYTFTNALLGTIKNNLRPNGFFALETHPQKSHEVAEWARGKNFEAVEVLNDLA
                     ARPHFVIGRLSVAQ"
     gene            1132..2037
                     /locus_tag="PLY93_07435"
     CDS             1132..2037
                     /locus_tag="PLY93_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69347.1"
                     /translation="MKTKYIFALLGLGTLFTIFFVFDNIFSIWEEASYARFRPVTTGL
                     HSNQIYRDVQVGAVSVDFYTAPLTQGTTIKRPLLVVVHGGFFQGGNKKNYAYLGGLGV
                     RSGYVVAIVQLPRYPGIFTKPFYSNTALKSRSLPAQAKQFAQFLQDVKSLENTYNFDT
                     ARVHILTHGSGSLLLGEADVTAFKSVVLVSPLYSLIEHVTDIAPVQLRAIDGYINDAE
                     ATRLSPLVWLKKTKEPVLLICAERDLPYIKKACQSADLTRAGSTPIQRVIVKKPSHFE
                     LMFHLGSKIEEATDALKKFLFDNAR"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2284..3213)
                     /locus_tag="PLY93_07440"
     CDS             complement(2284..3213)
                     /locus_tag="PLY93_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP69348.1"
                     /translation="MVAPKKNFSGDNMKKLIGIFAAIALVGAVSAAPKKAPAKPAPKA
                     AAPAPAAVAPAPAAVAPTAKSASNGIGLFVELRGGYILSNGSSTSQADGDTTGTKTYK
                     LSNAGGIAGGLTIGYAISKNLGLAASFDYRNASSRTWEQKAATSTSKIQNVKNTMVVG
                     LGLRPSVEAWGGTVYAGAGAALVLPYSDTTTIDITGAGATAGSTAKVEVKNEWNLGIG
                     VYGELGYNYNITDNLYLGVGVRALVITSNNDGKKQTVTHTTSGGFGPALAGGSGFAAG
                     ETEYSTSSAGGKAAYKSDGTTDIGFNINVGFRF"
     gene            complement(3310..3723)
                     /locus_tag="PLY93_07445"
     CDS             complement(3310..3723)
                     /locus_tag="PLY93_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRP69349.1"
                     /translation="MNAVRHLRLQNQIFRFVATYYQKESGAKNARATLENNEKSGAHN
                     PFTEADLSKFTEHVTDYCTFTRCELSSDGSFAKIYVSVWGSSKEQERIMKEIKRMVPD
                     MRTRIAKNIRMQAIPHLAIIQDHSFEKGSEIEKLL"
     gene            complement(3730..>4680)
                     /locus_tag="PLY93_07450"
     CDS             complement(3730..>4680)
                     /locus_tag="PLY93_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRP69350.1"
                     /translation="PVATILVRYRTLHVVDPFVVGLEGGKIRAMYNDKGQTVKEAGPA
                     TPVEILGLSGVPNSGDVINVMASEREAREIADKRQELARQQQAQQVKKVKLENFDATI
                     EEGKLKEYKVIIKGDVKGSVEALSAALEKMSNEEVRVRVIMGSTGEITESDVMLASAA
                     KATIITFNARANAKVRDIAAKESVEIKNYSIIYQAIDDMKLALGGLLSPDVSEKINGE
                     AEVRQVFKVSGMGSVAGSMVRSGFIKRNNPVRVLRDGAVIYTGSLKSLKRFQDDASEV
                     KEGFECGILVENFNDVKIGDIIESFEKVETQRVFEETPNA"
BASE COUNT         1167 a         1121 c         1069 g         1313 t
ORIGIN      
        1 gtatcttttt tttgaatttt ataacgacgc cacacaacgt gcggcacacg accatcggcg
       61 actgtacgca aattttgggt gacctcggcg cgcatttttt tcagctcggg gtcgttcgag
      121 gaaagcgagt gcaagtattt ttggccgcta cttgcaaaca aagcggttgc aataaaaata
      181 aaaaaggata accggcatcc cactgttttt ttaatcggca tttaccgagt aaccttgact
      241 ataattatgt ctcttgtcga attcaatccc cattttgatc gatgttttgc gtaaagctga
      301 aaatttttta gctgagaaaa aaatccccag cccacgttta gaagctcagc ttatttttgc
      361 gcattttttg aatctaaagc gaattgaaat ttttacgcaa agcgaacggc cgcttaatgc
      421 aaccgaactt gaaatgctgc gccaagcgtt acgcgaaaaa attacaggta aacccaccgc
      481 gtatatttta ggacaaaagg acttttacac gcgcactttt aaagtcgata gtacagtcct
      541 catccctcgc cccgaaaccg aagagctggt ggagcacgta ctcaaaaaaa tccccagcac
      601 aaaacacatc gttgacttgg gaacgggatc ggggtgcatc ggcgtgacgc tggcggcgga
      661 aagtgggtgc gaacgcatca ccttggttga tatttcacgt ggcgcacttt caatcgcaga
      721 ggaaaacgca gcacggcata tcgatggcgc aaaaacaaaa attgtcgtta tcgacgggga
      781 tttcacacag ccttctttta ttctctccga aaaagcagat gtgattgtga cgaatccgcc
      841 ttatgtactc gccgaagagt ttaattcact cgatgaaaca gtgcgttcgt tcgagccgcg
      901 tttggcgctc gtcgccgacg atttctacac ttttacgaat gcgcttttag gtacgataaa
      961 aaataatttg cgccccaacg gtttttttgc gttagaaaca cacccccaaa aatcgcacga
     1021 ggtagcagaa tgggcacggg gaaaaaattt cgaggctgtc gaagttttga atgacttggc
     1081 cgcacgaccg cattttgtaa ttggtcgttt gagcgtggct caataacatt tatgaaaaca
     1141 aaatatattt ttgccttgtt gggactcggc accctcttca cgattttttt cgtattcgac
     1201 aatatttttt cgatctggga agaagcttct tacgcacgtt ttcgccccgt gacaaccggt
     1261 cttcactcca accaaattta tagagacgtc caagtcggcg cggtgagcgt tgatttttat
     1321 actgcacctc tcacgcaggg cacgacaatt aagcgtcccc ttttagtggt ggtacacggt
     1381 ggttttttcc aaggcggcaa taaaaaaaat tacgcatact taggcgggtt gggggtacgg
     1441 agcggttatg tcgtcgcgat tgtgcaactg ccccgctacc cggggatttt cacaaaacct
     1501 ttttattcaa acaccgcgct caaaagccgc tctttacctg cacaagcaaa gcagtttgct
     1561 cagtttttac aagacgtaaa aagtttggag aatacctata acttcgatac cgcgcgcgtg
     1621 catattctaa cgcatggttc tggttcgctc cttttggggg aagccgatgt gaccgcgttc
     1681 aagagcgttg tactcgtgtc gcctctttac tcgctcatcg aacacgtcac cgatattgct
     1741 ccggtacaac tgcgcgcaat cgacggttat atcaacgacg cagaggccac tcggttgtcg
     1801 ccattggtat ggcttaaaaa aacgaaagag cccgttctgc tgatatgcgc cgaacgcgat
     1861 ttaccttata taaagaaagc atgccaaagc gccgatttaa cgcgcgccgg atcaacgccg
     1921 attcaacgcg tcatcgtaaa aaagccgtcg cactttgaac tcatgtttca cttgggtagc
     1981 aaaattgaag aagccaccga tgcgttgaag aaatttctgt tcgataatgc gcgttgagga
     2041 gggatggata accatccata cagccgcgct catctttatc ggcgcacagt attttgtcgc
     2101 aaacaccaca ggaagaaaac cgttgtgaat gaacgattcg agattatata attttaaggn
     2161 nnnnnnnnnt gttcggtatg ggcttaagcc cataccgaac aattaataat cgattggatt
     2221 gttatcgcgt cagatgcggg gttaaaccgc acctgacaat ctaactaacg agaattcgat
     2281 tcgttaaaac cggaaaccaa cgttgatgtt gaaaccaatg tcagttgtac cgtcagattt
     2341 gtaagcagct ttaccaccag cacttgatgt gctgtattct gtttcacctg ctgcgaagcc
     2401 tgatccacct gcaagagctg gaccaaatcc accgctagta gtgtgcgtta cagtttgttt
     2461 ttttccatcg ttatttgatg ttataacaag agcacggaca ccaacgccca agtaaagatt
     2521 atcagtgata ttgtaattat agcctaactc accataaaca ccaatgccta agttccattc
     2581 attttttact tcgactttag cagttgaacc tgctgttgca ccagcaccag taatgtcaat
     2641 ggttgttgtg tctgaataag gtaacactaa tgctgcacca gcaccagcgt agactgtacc
     2701 accccatgct tcaacagagg gacgaagacc taaacctaca accattgtat tcttcacgtt
     2761 ttgaatttta gatgttgatg ttgctgcttt ttgctcccat gtgcgtgatg aggcattgcg
     2821 ataatcaaac gatgctgcca aaccgaggtt tttggaaata gcgtaaccaa ttgtcaaacc
     2881 accagcgata ccgcctgcat ttgataattt gtatgttttt gttccggttg tatcaccatc
     2941 tgcttgtgaa gtgctcgaac cgttgctgag gatgtaacca ccgcgtaatt ctacaaacaa
     3001 gccaattccg ttgcttgcgc tttttgctgt gggtgctact gcggcgggtg ccggtgctac
     3061 tgctgcggga gcaggtgctg ctgctttagg tgctggtttt gcgggtgcct ttttgggggc
     3121 cgctgaaact gcgcctacaa gagcgatagc agcgaaaata ccaataagtt ttttcatgtt
     3181 atctcctgaa aagttttttt tgggagccac caccgccgcg tgtgcggcgt cgataattcc
     3241 tcattaaagt tactacgttt ttatgtcgat aaaccggcaa gtttttttta ttttctaaaa
     3301 ccggggccat caaaggagtt tttcgatctc gctgcctttt tcaaacgaat gatcttggat
     3361 aatcgccagg tggggtatcg cctgcatgcg tatatttttg gcgatgcgtg tgcgcatgtc
     3421 gggcaccata cgtttaattt ctttcatgat gcgttcttgt tctttggaag aaccccatac
     3481 gctcacgtaa atttttgcaa acgaaccgtc gctcgacagc tcgcaacgcg tgaacgtgca
     3541 gtagtcggtg acgtgttcgg tgaacttgga taaatcggct tcggtaaaag ggttgtgcgc
     3601 tccagatttt tcgttatttt ctaacgtggc acgcgcattt tttgcccccg actctttttg
     3661 gtaatacgtc gccacaaaac ggaaaatttg gttttgtagg cgtaagtgtc taactgcgtt
     3721 cacttgtgtt tatgcgttag gagtctcttc aaaaacgcgt tgggtttcaa ctttttcgaa
     3781 cgactcgatg atatccccaa ttttaacgtc gttaaaattt tcaacgagaa taccgcactc
     3841 aaagccttct ttcacttcgc tcgcatcgtc ttgaaaacgc ttgagcgatt tcaaagaacc
     3901 ggtataaatc accgcgccgt cgcgcagcac gcgcacgggg ttgttgcgct tgataaaacc
     3961 cgagcgtacc attgaacctg ccaccgagcc catgccggaa actttgaaaa cttgccgcac
     4021 ctcggcttcg ccgttgattt tctctgaaac gtcgggcgag agcaaaccgc ccaacgccaa
     4081 cttcatgtcg tcgatcgctt ggtagatgat gctataattt ttgatttcaa cgctttcttt
     4141 tgcggcaatg tcgcgcactt tggcgttcgc gcgcgcgttg aaggtgataa tcgtcgcttt
     4201 cgccgccgac gcgagcatca catcgctttc ggtgatttca ccggttgaac ccatgatgac
     4261 acgtacgcgg acttcttcgt tcgacatttt ttccaacgcg gcggagagag cctccaccga
     4321 acctttaacg tcgcctttaa tgatgacttt gtattctttg agtttacctt cttcgatggt
     4381 ggcgtcaaaa ttttcgagtt tcactttttt aacttgctgc gcctgttgct gacgcgcaag
     4441 ctcttgccgt ttgtcggcaa tttcgcgcgc ttcgcgttcg gacgccatga cgttaatcac
     4501 atcgcccgaa ttgggcacac cggacagacc taaaatttca acaggggtcg cagggcctgc
     4561 ctctttaacg gtttggccct tgtcgttata catcgcacgg attttaccgc cttcaagacc
     4621 gacaacaaaa ggatcgacga cgtgcagcgt tcggtagcgc actaaaattg tcgcaactgg
//
LOCUS       DAOMPD010000183         4670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7704_length_4670_cov_2.812784, whole genome shotgun sequence.
ACCESSION   DAOMPD010000183 DAOMPD010000000
VERSION     DAOMPD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7704_length_4670_cov_2.812784"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLY93_07455"
     CDS             complement(<1..577)
                     /locus_tag="PLY93_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69351.1"
                     /translation="MQHSITLNDLYDAIHPITKRYEFARALFIETSGLTVSSNLNQTL
                     IEPLPTTRGVVVSILKNGLLYEASSIVHSRNDIQKTIEGLENTVKKSNQEGTLRLMAE
                     ERANICAGNFIEDTLSNKDKAIYAMKMAQAIKAQNAKVAMASARYRQINTHEMYVSAE
                     KTLEQYLPRFEAVYAAVLKDTTTNATAQIYDG"
     gene            complement(577..1269)
                     /locus_tag="PLY93_07460"
     CDS             complement(577..1269)
                     /locus_tag="PLY93_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRP69352.1"
                     /translation="MKILFACLFVLAFLSCNKKNLLLKEGSISLVKGNVFTEAKTGKH
                     EKKSTPLHLGDKVHTGETIVTGEASVAILDLGAVQVEIQHNTKFTLEALESNAQVYLQ
                     EGSVWTHADKLQPMQKFALRTPNTIAAVRGTKFYTSTDGKTTGTCHCKGKIAFKNNIS
                     GYENINDSDYQLFYRDKKAVKVTPDDQRRWGITPTHNHSEIENSPLGKKNTLTAAEIA
                     KIKSYIEKQFNL"
     gene            complement(1313..1978)
                     /locus_tag="PLY93_07465"
     CDS             complement(1313..1978)
                     /locus_tag="PLY93_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HRP69353.1"
                     /translation="MKNFFYRVCLFVLSVVCVTPQNADDPIKKRNPNKVDDFADIYER
                     IYRKEMLQNFESDALEDIFEPVKSNADVFLSDELPPPITESQRYTVVRLRSSEVRRVR
                     LEFKTPLNIERHCRAFTLWLNVEKIRARLSLIVEDREGTRYFLDGGLLEFRGWRKIRF
                     TVGGRIRQYDLYLNEKAALKLKGIEIVFAQNYTKGKLPILLIDEIAVETRMKYQIPPA
                     LRK"
     gene            complement(1975..>2942)
                     /locus_tag="PLY93_07470"
     CDS             complement(1975..>2942)
                     /locus_tag="PLY93_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRP69354.1"
                     /translation="VLERKKQTITHDSFYNLGHYLKPNDAIFYNATRVEERRVSLVRQ
                     TTDKIFECIFLKKIKRDFSLSKNNAREEWQVLMRNIRRVRDGEIFYAQSDNAQTFIFH
                     RNDQELFLSSTNPLDAAAFARIGEMPIPPYMQRAASADEARTYQNFFREQMHEHEKIE
                     GSAASPTAALHFTPALYKSLQKQRIAFHPVCLDIGYGTFAPLTEENFAHATLHSEHYF
                     IPNESALAFTQAARKIVLGTTALRALLSWQRLGAIEGETQLFIRPENSVTEIDGLITN
                     FHLPQSSLLLLTAAFAGIDLVARAYQAAIDEGYRFFSYGDAMLIL"
     assembly_gap    2943..2952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3049..4503)
                     /locus_tag="PLY93_07475"
     CDS             complement(3049..4503)
                     /locus_tag="PLY93_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRP69355.1"
                     /translation="MKSTFLSYAIGVASVVRVFLQLFAQRVAYFFHQYASNLLIALVI
                     FLITLIAAHHLFPGALGKLYAQLDAQNSSTAQAWRTFDARCSLSFCRYAFPSPIRFYE
                     LEVKEQVGFQSYLAALKAERAKKKQNSASTAAQKFISAYTPSRIAAYGGNPTAAAPEN
                     ITAIYLTGTSARPAKVDNYLSFNRTQKMNGVVFDMKDVYGFVNYRSALPEVKLTQGKI
                     PPPLGELSPLVAYLKSQKIYTIARVTMFQDELMAMKFPERQIKLQSGGPLLTKGRTVW
                     VDPNKAAVQEYNLRIIREVLQAGVDEIQLDYIRYPAEGDWKIANYHDLKDFADKPVVL
                     GSFLQRVHSLTRAYGAHLSLDVFGVVAWQEALDIKSTGQNMHILAQTADVVSPMLYPS
                     HFGHAFGGVTNPADKPALFLEEGVAKLRAINAHVVIRPWLQAFKWRVTNYTTQYITEQ
                     IRGSQKAGGQGYLLWNAGNVYEHFTEPKIQKAQK"
BASE COUNT         1107 a         1018 c         1204 g         1331 t
ORIGIN      
        1 aaccatcgta aatttgtgcg gtagcatttg tcgtggtgtc cttgagcacc gctgcataaa
       61 ctgcctcgaa gcggggtaag tattgttcga gcgtcttctc tgcgctcacg tacatttcat
      121 gcgtattgat ttgacgataa cgcgccgaag ccatggcaac ttttgcgttc tgtgctttaa
      181 tcgcctgcgc cattttcatt gcatagatag ccttgtcttt gttggagagc gtatcttcga
      241 taaaattccc cgcgcaaatg ttggcgcgct cttctgccat gaggcgtagc gttccttctt
      301 gattactctt tttgacggta ttctctaaac cttcaattgt cttttggatg tcatttctgc
      361 tatggacaat cgacgacgct tcgtagagaa gtccgttttt aagaatgctc accacaacgc
      421 cgcgcgttgt aggcaatggc tcgatcaaag tttggttgag gttgctcgaa acggtgagac
      481 cggaggtttc gataaaaagc gcgcgtgcaa attcatagcg cttcgtgatg ggatgtatcg
      541 cgtcgtataa atcgttcaga gtgatggagt gttgcactat aaattaaatt gtttttcgat
      601 atacgactta atttttgcaa tttccgccgc ggtgagagtg ttctttttgc ctaagggact
      661 attttcaatt tcagaatggt tgtgcgtcgg tgtgatgccc catcgacgtt gatcgtcggg
      721 cgtgactttg acggcttttt tgtcgcggta aaaaagttga taatcgctat cgttgatgtt
      781 ttcgtaaccg ctgatgttgt ttttaaaagc gattttcccc ttgcagtggc aagtaccggt
      841 agttttgcca tcggtcgagg tatagaactt agtgccgcgt actgccgcga tggtgtttgg
      901 cgtgcggagt gcaaattttt gcatgggttg caatttgtca gcgtgcgtcc acacgctgcc
      961 ctcttgtaaa tacacttggg cgttgctttc gagagcttct aaagtaaatt ttgtattgtg
     1021 ctgaatttca acttgaactg ctcccaaatc gagaattgcc acacttgcct caccggtcac
     1081 gatggtctca ccggtgtgga ctttatcgcc caagtgtaag ggggtggatt ttttttcgtg
     1141 ctttcccgtc tttgcttcgg taaacacatt acctttaacc aaacttattg aaccctcttt
     1201 caataataga ttttttttat tacaagagag aaacgcgagt acgaataggc aagcgaagag
     1261 aatcttcata ccacattttg cagtgtaatg aaatcacgtc aagcatttac aattattttc
     1321 tcagcgctgg tggaatttga tatttcatac gcgtctcgac ggcgatttcg tcgatgagga
     1381 gtatcggcaa tttccctttg gtgtagtttt gagcgaacac aatttcaatc ccttttagtt
     1441 taagcgccgc tttttcgttg agatataaat cgtactggcg tatacgtcca cccacagtaa
     1501 aacgaatttt gcgccaaccg cgaaactcta aaagaccacc atctaaaaaa taccgtgtgc
     1561 cctcgcggtc ttcaacgatg agagaaagac gagcgcgtat tttttcgacg ttgagccata
     1621 gcgtaaaagc gcggcaatgc ctctcgatgt ttagaggcgt tttgaattcc aagcgcactc
     1681 tgcgtacttc ggacgaacgt aaacgcacga cagtatatct ttgcgattcg gtaatgggcg
     1741 gtggcagttc gtctgagaga aaaacgtcgg cgttactttt gaccggctca aaaatatcct
     1801 cgagtgcatc actttcaaaa ttttgtaaca tctcttttct gtatatacgt tcgtagatgt
     1861 cggcaaaatc atccactttg ttggggtttc tttttttgat gggatcatcc gcattctgcg
     1921 gggtgacgca caccacgctc aatacaaaaa ggcaaacgcg ataaaagaaa tttttcacag
     1981 tataagcatt gcgtcgccgt acgaaaagaa tcggtaacct tcgtcgatgg ccgcttgata
     2041 cgcacgcgcc actaagtcta tacccgcaaa tgccgccgtc aagagtaata aactcgattg
     2101 cggcaaatga aaattggtga tgagaccgtc aatttccgtg acgctgtttt cggggcggat
     2161 gaaaagctgt gtctctccct cgattgcgcc caagcgttgc cacgacaaaa gcgcacgcag
     2221 cgcggtggtc cccaacacaa ttttacgcgc agcttgggta aatgccaaag cgctttcgtt
     2281 ggggatgaaa tagtgttcgc tgtgtagcgt tgcgtgtgcg aagttttctt cggtgagcgg
     2341 cgcgaatgtg ccgtaaccaa tatccaggca cactggatga aatgcgatgc gctgcttttg
     2401 gagcgatttg taaagcgcgg gagtaaagtg gagcgcggcg gtgggtgacg ctgccgagcc
     2461 ttcgattttt tcatgttcat gcatttgctc gcggaaaaaa ttttggtatg ttcttgcttc
     2521 gtctgcgctc gccgcgcgtt gcatatacgg tgggatgggc atttctccga tacgcgcaaa
     2581 cgccgctgcg tcgagaggat ttgttgatga gagaaataat tcctggtcgt tgcggtgaaa
     2641 aataaacgtc tgtgcgttgt cactttgtgc gtaaaaaatt tcaccgtcac gcacgcggcg
     2701 gatgtttcgc atgaggactt gccattcttc acgggcgtta ttttttgata aggagaagtc
     2761 tcgctttatt tttttgagaa agatacactc aaaaattttg tccgttgttt gccgcacgag
     2821 agaaaccctg cgctcttcga cgcgggtggc gttgtaaaaa attgcatcgt tgggtttaag
     2881 ataatgtccc aaattgtaaa aagagtcgtg cgtgatggtt tgttttttgc gttcaagcac
     2941 cannnnnnnn nngccgtgca tcgtcgggcg gatggacact ataccttgcg atacgttcgt
     3001 cgggtaaatg aaaatcgagt gcgctgtact cggccggcca gtgcgctttc atttctgcgc
     3061 tttttgtatt tttggttcgg taaaatgttc gtatacgttg cccgcattcc acaagagata
     3121 accttgtccg ccggcttttt gcgaaccacg gatttgctcg gtgatgtatt gcgtcgtgta
     3181 gttggtcacg cgccacttga acgcttgcag ccacggacgg atgacaacgt gtgcgttgat
     3241 ggcgcgtagc ttcgcgacac cttcttctaa aaaaagtgca ggtttgtctg caggattagt
     3301 gacgcctcca aacgcgtggc caaaatgcga agggtagagc atcggtgaaa ccacatcggc
     3361 agtttgagcg aggatgtgca tattttgccc tgtacttttg atatcgagcg cctcttgcca
     3421 tgcaacgaca ccgaacacat cgagcgacaa gtgcgcgcca taagcgcggg tgagcgaatg
     3481 cacccgctgt aaaaacgaac cgagcacaac cggtttatcg gcaaaatctt tcaggtcatg
     3541 gtagttggca attttccagt cgccttcggc aggatagcgg atataatcga gttggatttc
     3601 atcgacaccc gcttgtaaca cttcgcggat gatacgcaag ttgtactctt gtacggcggc
     3661 cttgttggga tccacccaca ccgtacgtcc tttggtgagt aaaggtcctc ccgattgaag
     3721 tttgatttgc ctctcaggaa acttcatcgc catgagttca tcttggaaca ttgttacgcg
     3781 cgcgatggtg taaatttttt gcgactttaa atacgcgaca aggggagaaa gctctcccaa
     3841 aggcggcggt attttacctt gcgtgagctt tacttcgggc aacgccgatc tatagttcac
     3901 aaagccgtac acgtctttca tgtcgaagac aacgccgttc attttttgtg tacggttaaa
     3961 cgaaagatag ttatccactt ttgcggggcg agccgatgtg ccggttaaat aaattgcagt
     4021 gatgttttcc ggtgcagcgg cagtgggatt tcccccgtac gcagcgatac ggctgggggt
     4081 ataagcagaa ataaattttt gcgcagcggt agaagcggag ttttgttttt tctttgcgcg
     4141 ttcggctttc aacgccgcaa gatacgattg gaatcccact tgctctttga cttccaattc
     4201 ataaaaccga attggcgaag ggaaggcgta gcggcaaaac gaaagtgagc aacgggcgtc
     4261 gaaggtgcgc catgcttgtg cggtggagga attttgggcg tcgagttgtg cgtagagttt
     4321 gccgagtgcg cctggaaaaa gatggtgcgc tgcgatgagc gtaatcaaga agataacgag
     4381 tgcgattaaa aggttagaag cgtactggtg aaaaaaatat gctacccttt gcgcaaagag
     4441 ttggagaaac acgcgcacca ccgatgcgac gccgatggcg taagataaaa atgttgattt
     4501 cacactgaca ggggtggtgg ttgaaacagc gggtaaagcg ttgaattgac gagacaaagt
     4561 ttgccccgtt gagtaaggac tttaattttt atgaatcgtt atgtgagctc tatagttcca
     4621 tgaaaataac gagtttgtcc ttcggccaag gggtgattgg gacttcctcg
//
LOCUS       DAOMPD010000184         4635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7778_length_4635_cov_2.853057, whole genome shotgun sequence.
ACCESSION   DAOMPD010000184 DAOMPD010000000
VERSION     DAOMPD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7778_length_4635_cov_2.853057"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLY93_07480"
     CDS             complement(<1..339)
                     /locus_tag="PLY93_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRP69356.1"
                     /translation="MADLPKGVAELQAIIIAERKFFQEKISNSENENNSLREELRQLK
                     RFIFAHKSEKWIAEDKRQMLIFNEIEVIAVKEKKRIHIKGHSRKKAGRKALADHIPRV
                     EITHDLKSEEK"
     gene            complement(413..763)
                     /gene="tnpB"
                     /locus_tag="PLY93_07485"
     CDS             complement(413..763)
                     /gene="tnpB"
                     /locus_tag="PLY93_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HRP69357.1"
                     /translation="MLLADLSGFRIFIKPGHSDMRKGINGLALIVQERMGLDPIEHSI
                     FLFCNKRRNLLKVLYWDDNGFWLWLKRLEKHRFPWPKDSTTSREISREQLQMILSGID
                     FFNAHEKLNYARVG"
     gene            complement(766..1071)
                     /locus_tag="PLY93_07490"
     CDS             complement(766..1071)
                     /locus_tag="PLY93_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69358.1"
                     /translation="MNKKRNHARWAIWQKRIEAWQASGLMQSEFCREQGIAPRHFSLW
                     KRKLETTQTDTIENGFVEIPASTTKLSTAALETTLRINIGKFVVNVGSNEIVITPVG"
     gene            complement(1124..1480)
                     /locus_tag="PLY93_07495"
     CDS             complement(1124..1480)
                     /locus_tag="PLY93_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69359.1"
                     /translation="MSDNDFLSHLSNIAKTMAAWIEKARESGRKDDEIKYQMYDFCHD
                     EKHDAEQLTDRIVNAVRTYCEKYAESELSKYYLNFFSAVNNLVRHEYDYGVDMFDAAA
                     ATVSGDAGFRSYIISM"
     gene            complement(1563..1832)
                     /locus_tag="PLY93_07500"
     CDS             complement(1563..1832)
                     /locus_tag="PLY93_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69360.1"
                     /translation="MAKFVELMPARDLTNDERKILTALLSHDFPGVEPLREQLSKVKV
                     SGEFQDVKDFTIRLSVLRDTPKALVNRRIPVEAEKKYKNHDFYMK"
     gene            complement(1848..2231)
                     /locus_tag="PLY93_07505"
     CDS             complement(1848..2231)
                     /locus_tag="PLY93_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69361.1"
                     /translation="MNKQILKDRILAECDEDDVGLWVIIWELENQGVQNRHLKEETLT
                     FIELLMNDGLISAGHVRVVEQENRAVFDHWNLTPSETVARISKEWDKLGRLPTIGDIV
                     YFTITDKGMEYLRTIANDTPNSESV"
     gene            complement(2228..2737)
                     /locus_tag="PLY93_07510"
     CDS             complement(2228..2737)
                     /locus_tag="PLY93_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69362.1"
                     /translation="MAKFITNAGSSVVNANNNFLAYAKAAGLTVAVVAVIVLAVKFKN
                     WGAARAAAGQTGMNVAQALAGKGGNSVNNIMNILKPGGSLIGSPGTSSNIRMMDGGLS
                     AAQNLFSKLTQGGTLIQSSSYSGQLYQLANGGTVGLRTLSTRSGKNIVATIDVNLPGW
                     KGIREIKFK"
     gene            complement(2743..3240)
                     /locus_tag="PLY93_07515"
     CDS             complement(2743..3240)
                     /locus_tag="PLY93_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRP69363.1"
                     /translation="MLETYQSPATNELEIIQVIDPSFGSEIQSKIDAENSRFFFRILD
                     LASINDKKNLLQYVARKLNFPEYFGENWDAVDECLIDMPSYSPMVKSFLILVRNVEHL
                     FLHGKRELLTFLAALQFAGNNINFQDKKPFYVFLECLAGLSKQYLTEFCQKNLNEKNS
                     FLDGK"
     gene            complement(3242..>4635)
                     /locus_tag="PLY93_07520"
     CDS             complement(3242..>4635)
                     /locus_tag="PLY93_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRP69364.1"
                     /translation="THPVGSRSEQGDGGMSFGQRATAVVMLVAFVSVNCGSGFAPGSV
                     PSDLADNKEIIISALYTGLPTGTVYYSHNHLGSGALVTDENGDEIFRITYTEYGEIDL
                     HNSGKYNSETGEIEHHIDAALIAITAVKYTGQEYDPETGFYYYNARYYDPQLGLFTTP
                     DTVFDQGAGSFGFNRHMYVGGNPIMYSDPTGHNAEEAGRWDEKGNERVQESSQSESGW
                     QRNEERSGKSEASGVESARESQGGSSAREHADVDRGDRGERAAASSSNASDNSQLNQM
                     ISSITSTQFDTGSTGGTPLLIAQAAPVPVTTPGSGPVATDTGSSTADTNGDLPTYAKF
                     AGVTVAVVAVIVLAVKFKNWGAARAAAGQTGMNVARALAGSNGTSSQALTQRIQQTLQ
                     NIKSGNLPFAKDGTIFLNREGLLPSRAAGYYREFTVQNPALKTRGMERIVTGQQGEVY
                     FTPNHYKNFIRIQ"
BASE COUNT         1184 a         1141 c          888 g         1422 t
ORIGIN      
        1 tttctcttcg ctctttaggt cgtgcgtaat ttcaacgcgt ggaatatgat ctgcgagagc
       61 tttacgaccc gcttttttgc gcgaatgacc tttaatgtga attctctttt tctctttcac
      121 tgcgataact tcgatttcgt tgaagataag catttgcctt ttgtcttcgg caatccattt
      181 ttcggatttg tgcgcaaaaa taaagcgctt gagctgacgg agttcttcgc gcagcgagtt
      241 attttcattc tctgaatttg aaattttctc ttgaaagaac tttctttcgg ctataatgat
      301 cgcttgtagt tctgcgacac ctttgggtaa atctgccaca agcgtttcga ttgatttcta
      361 taacacatct ctgaaataat ttcaagcatt atttttattt tttgtaaaaa aattatccga
      421 cgcgcgcata attgagtttt tcgtgtgcat taaaaaaatc tataccagaa agaatcatct
      481 gtaattgttc gcgagagatt tcgcgactcg ttgtgctatc tttgggccat ggaaatcgat
      541 gcttttctaa ccgcttcaac caaagccaga aaccattatc atcccaataa agtactttca
      601 ataaattgcg acgcttgttg caaaataaaa aaatactgtg ctctatcgga tctaaaccca
      661 tccgctcttg cacaatcaat gcaagaccgt taattccctt acgcatatca ctatgccccg
      721 gcttgatgaa aattctaaag cctgaaagat cggcgagcaa cattgttatc ctaccggcgt
      781 gattacaatt tcgttagacc caacattcac tacaaactta ccgatgttaa tacgcagagt
      841 agtttcaagc gccgcagtcg ataactttgt cgttgaagcg ggaatttcca caaagccatt
      901 ttcaattgtg tcggtttgag ttgtttcaag tttccgtttc caaagcgaaa aatgtcgagg
      961 tgctatacct tgctcgcgac agaattcaga ttgcatcagg ccactcgctt gccatgcttc
     1021 gatgcgtttt tgccaaattg cccagcgggc gtggtttcgt tttttattca taccagcttt
     1081 taacatgaaa aaattaattc gtcaagatgg tgagaatgga cgcttacata gaaataatat
     1141 aagaccgaaa gcccgcatca ccagacacgg ttgccgctgc ggcgtcaaac atatcgacac
     1201 catagtcgta ttcatgacgg actaaattgt ttaccgctga aaaaaagttt aagtaatatt
     1261 tcgacaattc gctctctgca tatttttcgc agtatgtacg aacggcgtta acaattctat
     1321 cggtcaactg ctcggcatca tgcttttcat catgacaaaa gtcatacatc tggtatttga
     1381 tttcatcatc ttttcgcccg ctttcacgcg ctttttcaat ccacgctgcc atggttttgg
     1441 cgatattcga gaggtgtgac aaaaaatcat tatcgctcat aatatttcta accggcggtc
     1501 gactcctcca tgcctgcaat tccattgact atgaatttct tacccacaat tatgccttgc
     1561 cgttatttca tgtagaaatc atggtttttg tatttttttt ccgcttcgac agggatacgt
     1621 ctattgacaa gggctttggg cgtatctcgt agtacagaca accgaatagt gaaatcttta
     1681 acatcttgaa actccccaga aacttttact ttactcaact gttcccttaa tggttcaact
     1741 cctggaaaat catgtgacaa tagagccgtt aaaattttgc gttcatcatt tgttaaatct
     1801 ctcgctggca ttagttctac gaatttagcc atgatataac cttagcctca gacactttct
     1861 gaatttggcg tatcatttgc gattgtccgc aaatactcca tccccttatc ggtgatggta
     1921 aaataaacta tatcgccaat cgtcggcaat cttcccaact tatcccattc tttgcttatt
     1981 ctggcaacgg tttcagatgg cgtcaaattc cagtggtcaa agacggctct gttttcttgt
     2041 tctacgactc tgacatgacc tgccgaaatc aaaccatcat tcatgagtaa ttcaatgaat
     2101 gtcaatgttt cttctttaag gtgtctgttt tgaacacctt ggttctctaa ttcccaaatg
     2161 atgacccata gtcctacatc gtcttcatca cattcggcaa gaattcgatc tttcaatatt
     2221 tgcttattca tttgaatttg atctctctta tacctttcca gccaggtaga tttacatcaa
     2281 ttgtcgctac aatatttttc ccgcttcgag ttgataatgt tcgtaggccg acagttcctc
     2341 catttgccaa ttgatatagt tgaccagaat atgaagaact ttgaataagg gttcctcctt
     2401 gtgttaattt gctgaacaaa ttctgagcgg cggataatcc gccatccatc atgcggatat
     2461 tgctgctggt acccggcgaa ccgataagac tcccgcccgg cttgaggatg ttcattatat
     2521 tgtttactga gttaccccct ttcccagcca atgcctgcgc gacattcata ccggtctgtc
     2581 ccgccgccgc tcgtgcggca ccccaattct taaacttaac cgcaagaacg ataaccgcca
     2641 caactgccac cgtaaggccg gcggctttag cgtaggccag aaaattgttg ttcgcattca
     2701 ctaccgaact tcccgcatta gttatgaatt tggccatcgt cgctacttgc catctaaaaa
     2761 agaatttttc tcattgagat ttttctgaca aaactccgta agatattgtt tcgataagcc
     2821 agctaagcat tctaaaaaaa cataaaatgg ctttttatcc tgaaagttta tgttattgcc
     2881 tgcaaattgg agagcggcta agaatgttaa caactcgcgt tttccatgga gaaacaaatg
     2941 ctccacgttt cttaccaaaa taagaaaact cttaaccata ggtgagtatg aaggcatatc
     3001 gattaggcat tcgtctactg catcccaatt ttccccgaaa tattccggaa agtttagttt
     3061 tcttgcaaca tattgtagaa ggtttttttt atcatttatg gaggctaagt caagaattct
     3121 aaaaaaaaat cgagaattct ctgcatcgat ttttgattga atttctgagc caaatgaggg
     3181 gtcaattact tgaattattt ctaattcatt tgtggccgga ctttgatatg tttcgagcat
     3241 tttattgtat cctaataaaa tttttgtagt ggttcggggt gaaatacact tcaccttgct
     3301 ggccggtaac aatgcgctcc attccccttg ttttaagagc tggattttgt actgtaaatt
     3361 cacgatagta acccgcagca cggcttggta aaaggccttc tcgatttaga aaaattgtcc
     3421 catctttagc aaaaggtaaa tttccacttt ttatattttg taatgtttgt tgaatccttt
     3481 gagttaaggc ttgactactt gtcccattag aacccgccaa cgcccgcgcc acattcatac
     3541 cggtctgtcc cgccgctgct cttgcggcac cccaattctt gaacttaacc gcaagaacga
     3601 tgaccgctac gactgccacc gtaaccccag caaatttcgc gtaggtcggc aaatcaccgt
     3661 tcgtatccgc cgtcgaactt cccgtatctg tggcgaccgg gccgctgccc ggcgtggtta
     3721 ccgggactgg cgcagcttgt gctataagta gcggcgtgcc gccggtactt cctgtatcga
     3781 attgcgttga agtgatggaa ctaatcatct gatttaactg cgaattatcg ctcgcattac
     3841 tactgcttgc cgcagccctc tcgcctctat cgccccggtc gacatccgca tgctcccgcg
     3901 cagaacttcc accctgcgat tcgcgggcgg actctacacc ggatgcttcg ctttttccac
     3961 tgcgttcttc attcctctgc caaccgctct ccgactgcga agattcctga accctttcat
     4021 tgcctttctc atcccaccta ccggcttctt cggcgttgtg gccggtgggg tcgctgtaca
     4081 tgatgggatt gccacccaca tacatgtgcc gattgaaacc aaaactccct gcgccttgat
     4141 cgaataccgt atccggcgtc gtaaacaacc ccaactgcgg gtcgtaataa cgagcgttgt
     4201 agtaataaaa ccccgtctcc ggatcatatt cctgcccagt gtatttgacc gccgtaatcg
     4261 caatgagcgc agcgtcgata tgatgctcaa tctctcccgt ctccgaatta tacttcccag
     4321 aattatgcag atcaatttcc ccatactcgg tgtacgtaat cctgaaaatc tcgtcgccgt
     4381 tctcgtcagt aacaagagca ccactaccta aatggttatg gctataatat accgtacccg
     4441 taggcaatcc cgtgtacagt gcagaaataa taatctcttt attatccgcc aaatcgctcg
     4501 gcacagagcc cggggcaaat ccgctaccgc agttgacact tacgaaagcg acgagcatga
     4561 caacagcggt ggcgcgttgt ccgaaactca tccccccgtc tccctgttcg ctacgcgaac
     4621 ccacggggtg agtta
//
LOCUS       DAOMPD010000185         4621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7811_length_4621_cov_2.358958, whole genome shotgun sequence.
ACCESSION   DAOMPD010000185 DAOMPD010000000
VERSION     DAOMPD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7811_length_4621_cov_2.358958"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PLY93_07525"
     CDS             complement(<1..1068)
                     /locus_tag="PLY93_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69365.1"
                     /translation="MKKKNLPPLLLASRDSLLALAQTIEAAIRLEKVGFTPKIAALKT
                     AGDLKLDSPLYRVADQGTKEGRAFFTRELDDALITGRADAAVHSFKDLPAEKIDGISN
                     PILFSETSTADILLSTDPLSLAEGGKNYTIGTSSLRRIHQLGFAWPYAKTVTLRGNIV
                     TRLRKLIEKHDSINAILIAEAGFKRMKEFMGIPVEKYAHFLDEATLEHLTIELEHFKT
                     YLSQKLYTHALPESKFPTAPGQGVLAFQFSEKERHKFTEHLDEIFPEHPRTAARALLE
                     RSVMTGLETGCHAPLGVSAHYTNGDTFHVSLCFSKSTTTSPVGFANSVFIERTTLSAD
                     ALVDEVRRPFETIYWWGLNDVT"
     gene            complement(1065..>1834)
                     /locus_tag="PLY93_07530"
     CDS             complement(1065..>1834)
                     /locus_tag="PLY93_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69366.1"
                     /translation="SLVEEAIHERQLAPVDDTLILVGASEMSAKVARFALRRGVKRFL
                     LVRKNANRAMNYEMQRLMNENPNLFTVATLSEFKENFALLNAQAIVLASTATEPLFTA
                     AELHRLNANQTLSENAAIVDLSLPANVADDVAEELAHRFISLSSLKKISDEAKATRAA
                     SALEAEPIIRRAVYQLWLDALYRENPMVVKEYLDNKTNQSDSEWQRLAVEASLSEKQK
                     RIMYDFLKKEQRRTLQRHREMILDLIAGTSEAPSFSL"
     assembly_gap    1835..1844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1845..2398)
                     /locus_tag="PLY93_07535"
     CDS             complement(<1845..2398)
                     /locus_tag="PLY93_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69367.1"
                     /translation="MPSIGFFQRFHAASLSIATHEVESLSPFPDSRRFYLESGWSLPP
                     EIKGLLYMATCNRIELYAEFTDGVSRDEGFTALSHLSPILKTMSTTNPYYMAGEKVLV
                     HLISVASGLKSLALGETQIAGQIKRDMGHSASRGWLSSGLSTLLAKALETQKKIRTTT
                     GISENSYSLMSLVDRICQHFEDSA"
     gene            complement(2484..3452)
                     /locus_tag="PLY93_07540"
     CDS             complement(2484..3452)
                     /locus_tag="PLY93_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRP69368.1"
                     /translation="MKKEIQKIKMGIIGVGHMGSYHLNVVTGMPQYELVGIFDARTDY
                     ANEKAEQFETSAYESYETLIQKCEAVTIAVPTHLHYGIAKYALENNVHVLVEKPITLD
                     IKEAEELIRLAREKKRILQVGHVERFNGAVMALANIASEPLLIQTRRLHPHNNRILDV
                     GVVLDLLIHDIDIVLNLVKRPLLKHAAVGSLVTGNHEDIAIIDLQFEGGCIATLTASR
                     LSQYKERSLTVTQKNNFVILNYGTQEIEIHRQGNSVTVTAPDEIKYSQESVVERVFVH
                     KDNPLKSEHEHFYNCILGKETPLVSGDKDLETLKIALTSIEQIKRG"
     gene            complement(3485..4589)
                     /gene="dnaJ"
                     /locus_tag="PLY93_07545"
                     /pseudo
     CDS             complement(3485..4589)
                     /gene="dnaJ"
                     /locus_tag="PLY93_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
BASE COUNT         1071 a         1101 c         1176 g         1263 t
ORIGIN      
        1 agtcacgtcg ttcaaccccc accaataaat cgtttcaaaa ggacgacgca cttcgtcgac
       61 gagggcatcg gcagaaagtg tggtgcgctc gataaaaaca gagttggcaa aaccaacggg
      121 acttgtcgtg gtgcttttac tgaaacagag cgaaacgtga aaggtatccc cgttggtgta
      181 atgcgctgag acgcctaaag gggcgtggca acccgtttca agcccagtca tcacgctacg
      241 ctccaaaagc gcacgcgctg cggtgcgtgg gtgttcggga aaaatttcgt ccaaatgctc
      301 ggtgaatttg tgccgttctt tttctgaaaa ttggaatgcc aaaacgcctt gcccgggagc
      361 ggtggggaat ttactttcag gtagcgcgtg tgtatagagc ttttggctta aatatgtttt
      421 gaagtgctct aattctatcg ttaagtgctc aagagtggct tcatctaaaa agtgagcgta
      481 tttttcaacc ggtatcccca taaattcttt catgcgttta aaacccgctt cggcgattaa
      541 aatagcattg atgctgtcgt gcttttcgat gagttttcgt aaccgggtga cgatgttgcc
      601 gcgcagcgtt accgtttttg cgtacggcca tgcaaaacca agctggtgta tgcgacgcaa
      661 ggaggatgta ccaatggtat aattttttcc accttcggca agcgaaaggg ggtctgtcga
      721 tagtaaaata tctgcagtgg atgtttctga aaaaagaatg gggtttgaaa taccgtcgat
      781 tttttcagcc ggtaaatctt taaacgaatg caccgccgcg tcggcgcgtc cggtgatgag
      841 cgcatcgtcg agctcacgag taaaaaaggc cctgccttct tttgtgcctt gatcggccac
      901 gcgatagagc ggcgaatcga gttttaaatc accggctgtt ttcagcgccg caattttcgg
      961 ggtaaatccc actttctcaa gccgtatggc cgcttcaatg gtttgcgcca acgcaagcag
     1021 cgaatcgcgt gaggcgagta agagtggcgg tagattcttt tttttcataa tgagaaggag
     1081 ggagcctcgc ttgtgcccgc aatgagatct aaaatcattt cacgatggcg ttgcaatgtg
     1141 cgccgctgtt ctttttttag aaagtcgtac attattcgtt tttgcttttc gctgagcgat
     1201 gcttcgacgg ccaagcgttg ccattcgctg tcgctttggt tggttttatt gtcgaggtat
     1261 tcttttacaa ccatgggatt ttcacgataa agcgcgtcga gccacagttg ataaacagca
     1321 cgacggataa tcggctcggc ttctaacgcc gacgccgccc gtgtcgcctt cgcctcgtct
     1381 gaaatttttt tgagcgacga gagtgaaata aaacggtgcg ctaactcttc ggcgacatcg
     1441 tcggcgacgt tggcagggag cgaaagatcg acaatcgctg cgttttctga taacgtttgg
     1501 tttgcgttga ggcggtgtaa ttcggcggct gtgaaaagcg gttcggttgc ggtcgatgcc
     1561 aacacgatcg cctgcgcatt caagagcgca aagttttctt tgaattcaga tagcgtcgcc
     1621 acagtgaaaa ggttgggatt ttcgttcatg agacgctgca tttcataatt catcgcacgg
     1681 tttgcatttt tacgcaccaa aagaaagcgc ttcaccccac ggcgtagagc aaagcgcgcc
     1741 actttggcgg acatttcaga agcccccact aaaataagag tgtcatccac gggtgcaagt
     1801 tggcgctcat ggatcgcttc ttcgacgagg ctcannnnnn nnnntcggca ctgtcttcga
     1861 agtgctggca gatgcgatcg acgaggctca tgagcgaata cgaattttct gagatgccgg
     1921 tcgttgtgcg gatttttttt tgtgtctcta acgctttggc taaaagagtc gataggcctg
     1981 atgaaagcca tccgcgcgat gccgagtggc ccatgtcgcg tttaatttgc cctgcgattt
     2041 gggtttctcc taacgccaag cttttaaggc cgctcgccac tgaaatgaga tggactaaca
     2101 ctttctctcc cgccatataa tacggattcg ttgtcgacat cgtttttaaa atcggcgata
     2161 aatgagataa agcggtgaag ccctcatcgc gtgaaacacc atcggtaaac tcagcataaa
     2221 gttcgatgcg gttgcaagtc gccatgtata agagtccttt aatttcaggc ggtagcgacc
     2281 atccgctttc taaataaaac cgacgcgagt cgggaaaagg agagagcgac tcgacttcgt
     2341 gcgtggcaat gctaagcgaa gcggcgtgga aacgttgaaa gaagcctatg gaaggcatag
     2401 ttagtagggt aatccgttaa aagtgtagtc ggcaaaaata taaagagggt gttgtgtcac
     2461 tgaattgtca gtttcacaaa cgattatccc cgtttaatct gctcgatact cgttaacgca
     2521 attttgagcg tttctaaatc tttgtcgccc gagacgagtg gcgtctcttt acctaaaata
     2581 caattataga aatgttcgtg ttcgcttttg agagggttgt ctttgtgcac aaacacccgc
     2641 tcgactaccg actcttgcga gtatttaatt tcgtcgggag cggtcacggt gactgaattt
     2701 ccttggcggt ggatttcaat ttcttgcgtg ccgtaattta agataacaaa attatttttt
     2761 tgcgttacag tgagcgagcg ctctttatac tgcgacaggc gcgaagccgt caaggttgcg
     2821 atacatccgc cctcaaattg taaatcgatg atggcaatgt cttcatgatt gccggtgaca
     2881 aggctcccca ccgccgcgtg ttttaagagc ggccgtttga cgagatttaa aacaatgtcg
     2941 atgtcgtgga tgagtaaatc gagcaccact cccacgtcga gaatgcggtt attgtgcggg
     3001 tgcagccgcc gcgtttggat gagtagcggc tccgatgcaa tattcgcgag cgccatcacg
     3061 gcgccattga aacgctcgac gtggcccact tgcaaaatcc gttttttttc gcgcgcaagg
     3121 cggattaact cttcggcttc tttaatgtcg agagtaatcg gtttttccac caacacatgt
     3181 acattgtttt ccagcgcata ctttgcaatg ccgtagtgca agtgcgttgg cacagctatt
     3241 gtgaccgctt cgcatttttg gatgagcgtt tcatacgact cgtacgcgct ggtttcaaat
     3301 tgctcggcct tctcgttggc gtagtcggtg cgtgcgtcaa aaataccgac gagttcgtat
     3361 tgtggcatac ctgtgacgac gttgagatgg taactaccca tgtgaccaac accgataatg
     3421 cccattttta ttttctgaat ttcttttttc atggatattg aaaatctgct gaagcaattt
     3481 tcctttagcg taacttacta aaaatacccg aacccgacgc ttccatttcc gcttcgagct
     3541 ctttcacgag gcctttggcc ttgcttgaaa gcgatttagg gatttctata cgtgcgtgcg
     3601 cgatgagatc gccgaaacgt ccttggccgc ccatgtaagg caagcctttt ccgcgcacac
     3661 ggaagagggt gtcgggttgc gtaccagatg gaattttcac tttaacttta cttttatcca
     3721 gcgtctcgac ggtcacttcg ccacccaaga tagcagtggt gacgggcacg ttgatataca
     3781 tcgccagatc gacgccgtcg cgttcaaatg ttttgtgttt atggatatgt accacaacgt
     3841 agagatcgcc cgatggtccc ccgcgtgggc ctgcttcgcc ttcgccggaa attttgagcc
     3901 gtgtacctgc gtcgatgcca gcagggatgc ggatcgcgag tttgcgtttc ttgcttacgg
     3961 taccttgacc actgcaagta cggcagcggt ttttaatgag tttgcctgtg ccgccgcagg
     4021 tgttgcatgt cgacgccacc gagacaaaac cggtcgaacg acgcacttgt cccgccccgc
     4081 cgcaaatgtg gcaagtgtcg agtttcccgt cggaggaacc ggtaccgcta caatcgccgc
     4141 agctttcttc gcgggggata actatctctt cttctttacc attataaacg tcttcgagag
     4201 aaatttctaa attatagcgc aaatcgctgc ctcggcgcac cgaatgagcc gaacgcgatc
     4261 gaccaaaata agttttcaaa aatatcttca aagctggtac ctgaaaaaat atcggagaag
     4321 tcgctatacg cggcttgacc aaacccttgc gcaccggcgc caccgacacc ggcatggccg
     4381 tattggtcgt agagtttgcg cttttgttcg tcgcgcaaaa cttcatacgc ttcggtggcc
     4441 tctttgaatt ttttctcggc ctcggcgttg ccggggtttt tatcgggatg gtactgcatc
     4501 gccagcttgc ggtatgccgc tttaatatcg tttaagctcg ctgacttggg tacacctaat
     4561 acttcgtaat aatcgcgttt atctgccatg ttgttattgc aaaaaactcc cccacgcaac
     4621 g
//
LOCUS       DAOMPD010000186         4615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7825_length_4615_cov_2.428509, whole genome shotgun sequence.
ACCESSION   DAOMPD010000186 DAOMPD010000000
VERSION     DAOMPD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_4615_cov_2.428509"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLY93_07550"
     CDS             <1..883
                     /locus_tag="PLY93_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRP69369.1"
                     /translation="QPVAYQFSAKEYDQETGLYYFGQRYYDPRVSTWVSTDPAIEKEF
                     LPDNGQADEGKIAGLGGVFNSTNLNTYAYVHHRPIIVTDPDGRYAQLIVFGGGALVGI
                     LSRAAIDQLNGQTSTWHDYLGSATTGGVTGLLLLRTNPVLAGIGGSAAGNVVGQVAKM
                     ADPGHQQSSFDWSSFGTDVGVATAFGVATQLPIIKDLFPKIAGITTGNNNSSALYKQI
                     STKLQNETIKNFTPKTAVKMLPGMTLKEQMLPSGLVGAHFSTEKPKGGRNGNGGGASS
                     KSSAPSWGLGVIPYADK"
     gene            924..1376
                     /locus_tag="PLY93_07555"
     CDS             924..1376
                     /locus_tag="PLY93_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69370.1"
                     /translation="MDAKKPQSTFAHYSTARSAYIETGLIFLPIAIAAIIVSVFREDW
                     SLFGASWLLIGFMLFLWLLFYDTQIVLTETGVTVGRFALRRKMLKYGEIDYIRLNFRL
                     VSIIPRLGIKKSPIRFYKRNLRKTDLENLLRQIKANCDKYKSRGDSKL"
     gene            1373..1528
                     /locus_tag="PLY93_07560"
     CDS             1373..1528
                     /locus_tag="PLY93_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69371.1"
                     /translation="MTTPWKPAKNQREAFKDEAKRIRGDDGGVKSGENYNKINSPGEK
                     YLKQDGQ"
     gene            1555..2412
                     /locus_tag="PLY93_07565"
     CDS             1555..2412
                     /locus_tag="PLY93_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69372.1"
                     /translation="MNIEVKNSGKYQVVRIDSENIAPCMQVYRDRQLDGISISPHLGY
                     KKRDLNFLKDYPDIKGIILGYADEIDLTHISCAKELEFITVTGNKQPMDYGQFPKLKE
                     LRLEWHKKINLPKVATQLKLLDLSAYKPSDKSLKSLPAAPNLERLELVQGNTISLDGI
                     EKYSRLLSAEFHYVRNLNDINAITAVRGLTSLKFHNCKNITDYSVLCKLQELELLYIH
                     NAGTIPSIKFVTQMKSLKTFRFIETAINDGDLTPLLRLKNIAFQNKKYYSHTQQEIRT
                     HIGQSIDEP"
     gene            2402..>2642
                     /locus_tag="PLY93_07570"
     CDS             2402..>2642
                     /locus_tag="PLY93_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69373.1"
                     /translation="MNRKTSGNIEFIEDFSLSIDTLYRETQRQYFLCLLKIYIAEFFG
                     ELFYFACRFHDKGVAENREALRAPRGTEARLAQSCA"
     assembly_gap    2643..2652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2755..>4615
                     /locus_tag="PLY93_07575"
     CDS             2755..>4615
                     /locus_tag="PLY93_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69374.1"
                     /translation="MLQTKKDASVAPGLSPGQQMPSNVMVVSGQVVFDALGRTVEQYY
                     PVTETKGAGNTTYNTAVDSIPPTRTLYDVLDRSVQTTLPDNTVTTSMYGFGQDRMGAL
                     QFQTRVSDALVNVKESYRDVKGLITSIKEFASGATIQTSYAYDALKQIIQVVDDKSNT
                     TLITYDNLGRRTRIDNPDTGRTDSVYDAAGNLVAKITETLRADNKQIEYDYDYNRLIK
                     IRYPIFTENNVTYSYGSAEQTGDAKGNRAGRVAMVTDASGNEERFYGPLGETIKEVKT
                     LPARWAWGFIDREYVTEYKYDTWNRLMELTYPGVEEDHVRPRLWTRFWFWWPFRPKVR
                     SVKEVLTYAYDSGGLVTKATGKKDQRTYPYLSRLDYDKFGQRIYQELGNGVKTTYTYK
                     SDNRRLENLQSQLPNPSQYKFQNLSYAYDAVGNITQIQNDIILPDTLPLFGNAGRVGH
                     RVGGPSTQNFGYDDLYRLTSATGTYQQTPWTTNRFTLSMQYDSIHNITQKNQLNEFVN
                     PGGAHRYFDTSYNYAYAYNATKPHAASDIGPYENLYDANGNLIRREHKNTHWMRHMVW
                     DEENRLACVREPWHGYMPQHPNSCFGFGPRFVYNAAGTRVLKDGFQRSFSINEH"
BASE COUNT         1356 a         1049 c         1096 g         1104 t
ORIGIN      
        1 gcagccggtg gcgtatcagt ttagtgcaaa agagtatgat caggagacgg ggctctatta
       61 ttttggtcag cgttattatg accccagagt atctacttgg gttagtacag atccggcgat
      121 tgaaaaagaa ttcttgccag ataatggcca agctgacgag gggaaaattg caggtttggg
      181 tggggtattc aactcaacaa atttgaacac ctatgcgtat gttcatcatc gaccaattat
      241 tgtgactgat cctgatggca gatacgcaca actcattgtc tttgggggcg gtgccttagt
      301 aggcatactt tcccgcgcag caattgacca acttaatggt caaactagca catggcatga
      361 ttatcttggc tcagcgacta cgggtggcgt cacaggctta ctcctgttgc gcacgaatcc
      421 tgtgcttgcg ggtattggtg gtagtgctgc cggtaatgtt gtcggtcaag tggcgaaaat
      481 ggccgatcct ggtcatcagc aatctagttt tgactggtca tcctttggga cggacgtagg
      541 cgtagctact gcattcggtg tcgcgacgca attaccaata ataaaggatt tgtttcctaa
      601 aattgctggt attacaacag gcaacaataa ttcttctgcc ctgtacaaac agatttcaac
      661 taaattgcag aatgaaacca tcaagaattt tacacccaag acagcagtca aaatgttgcc
      721 tggtatgacg ctaaaagaac aaatgctacc ctccggatta gttggggcac atttctccac
      781 tgagaaaccg aaaggaggtc gtaatggtaa tggtggtggt gcttcttcga aatcttcagc
      841 gccaagctgg gggcttggag taatccccta cgcggataaa taaccgttgg caacatattt
      901 tagagtcagt aaataattaa gagatggatg ccaaaaagcc acaatctaca ttcgctcatt
      961 actcgacggc ccgttcggcc tatattgaaa ccggcctaat ttttttgcca attgctattg
     1021 cggccataat tgtctctgtg tttcgagaag attggagctt atttggggcc agttggctgc
     1081 tcataggttt catgctgttt ctttggcttc tgttttatga tacacaaatc gtattaaccg
     1141 agactggcgt cacggttggt agatttgcct tgagacgcaa aatgttgaaa tatggagaaa
     1201 tcgactatat ccgattgaat tttcgactag tatcgattat tcccagatta ggtatcaaaa
     1261 aaagcccgat tagattttat aaacgtaatt tacggaagac ggaccttgaa aatttgctgc
     1321 ggcagattaa agctaattgt gataaataca aaagtcgtgg agactctaaa ttatgaccac
     1381 cccctggaaa ccagccaaaa atcaacgaga agcatttaaa gatgaagcga agaggattag
     1441 aggtgatgac ggcggtgtaa aaagcggtga gaattataat aaaatcaatt cgccaggaga
     1501 aaaatatctt aagcaagatg gccaataatt aaaattgccc taaagaagag catcatgaac
     1561 atcgaagtaa aaaattcagg caagtatcaa gttgtcagaa ttgactctga aaatattgct
     1621 ccttgtatgc aagtatatcg agatcgacag ctcgatggaa tttccatctc acctcatctc
     1681 ggctacaaga aacgagattt gaactttcta aaagactacc cagatattaa ggggattatt
     1741 ttaggttacg ctgatgagat tgatctgacc catatttctt gtgctaaaga actcgaattt
     1801 atcaccgtta caggcaataa acagcctatg gactatgggc aatttccaaa gctaaaagag
     1861 ttacgtttag aatggcataa aaaaattaac ttacccaaag tggcaacaca gctcaaactt
     1921 ttagacctta gcgcctataa gccatcagat aaatcattaa aaagcttacc cgcagcacca
     1981 aatttagaga ggttagagct agtccaagga aacaccattt cattagatgg aatagaaaaa
     2041 tattcgcggt tgctttctgc agaattccat tatgttagaa acttgaacga tataaatgca
     2101 attactgctg tcagaggctt aacatcatta aaatttcaca attgcaaaaa tatcactgat
     2161 tattctgtat tgtgtaaact gcaagaactg gaattattgt atatccacaa tgccggcaca
     2221 ataccatcaa tcaaatttgt cactcaaatg aagtcattaa agacatttcg ttttatagaa
     2281 acagcgatta acgatggtga cctaacaccc ttgttgagat taaaaaacat tgcctttcag
     2341 aataaaaaat attattcaca tacacagcag gaaataagaa cacacatagg acaaagcata
     2401 gatgaaccgt aagacatctg gtaatataga gtttatagaa gacttttctc tatctattga
     2461 taccttatac agagaaaccc aacggcagta ttttctttgc ttgcttaaga tttatatagc
     2521 ggaattcttc ggtgaattat tttatttcgc gtgccggttt catgacaagg gagtggcgga
     2581 gaaccgcgaa gctttgcgag cgccgagggg caccgaggcg cgtcttgcgc aaagctgtgc
     2641 ccnnnnnnnn nnagggccgc gggtcgcagc caggggggcg tgcccccctc atgcatcgat
     2701 acaattttgg gagcctgccc tgagcgaagc cgaagggtcg atggtctgaa gcgcgtgttg
     2761 cagacgaaga aagatgcatc tgttgccccg ggcttaagcc cagggcaaca gatgccaagc
     2821 aatgtgatgg tagtttcggg acaggttgtt ttcgacgcgt tgggtcgcac ggtcgagcaa
     2881 tactaccctg ttaccgaaac gaagggcgcg ggtaacacga cgtataacac cgccgtcgat
     2941 tcgataccgc cgacgcgcac tctatatgat gtactcgacc gcagcgtgca aacaacgttg
     3001 cccgacaaca ccgtgactac gtcgatgtat ggcttcggcc aagaccgtat gggcgccttg
     3061 cagtttcaaa cgcgcgtgtc cgatgcgctg gttaatgtga aagagagcta ccgcgacgtg
     3121 aaggggctca tcacttcgat taaagaattt gcatcgggtg caacaataca aaccagctac
     3181 gcatacgacg cgttgaagca gattatacaa gttgtcgatg ataaatcgaa tacgacgctg
     3241 attacctatg ataatctggg ccgccgcacg cggattgaca accctgacac agggcgtacc
     3301 gatagcgtgt acgacgcagc gggcaatctt gttgcgaaaa ttaccgaaac gctgcgcgcc
     3361 gataataaac agattgaata cgattacgat tataatcggc tcatcaaaat tcgctacccg
     3421 atttttacag agaacaacgt aacgtatagc tacggcagcg cagaacaaac gggtgacgca
     3481 aagggcaacc gtgcggggcg ggtggcgatg gtcaccgacg cttcgggtaa cgaggagcgt
     3541 ttttacggac cgctcggcga aactattaaa gaagtgaaaa cgctgccggc tcgttgggcg
     3601 tggggcttta tcgaccgcga gtatgtcacc gaatacaagt acgacacttg gaatcgtttg
     3661 atggagctca cgtacccggg cgtagaagaa gaccatgtac gcccgcgtct gtggacgcgc
     3721 ttttggttct ggtggccgtt tcgccccaaa gtgcgcagcg tgaaagaggt gctgacgtac
     3781 gcatacgatt cgggcggact tgtcaccaaa gcgaccggca agaaagacca gagaacgtat
     3841 ccgtaccttt cacggctcga ctatgataag tttggccaac gaatttacca agagcttggc
     3901 aacggcgtga agacgacgta cacttacaag tcagacaacc gtcgcttaga aaacttgcag
     3961 tcgcaactgc cgaacccaag ccaatacaaa tttcaaaact tatcgtacgc gtacgacgcc
     4021 gtgggcaaca tcacgcagat tcaaaacgac atcatattgc ccgacacgct acccctattc
     4081 ggcaatgcag gacgcgtcgg ccaccgtgtc ggtgggccga gcacgcagaa ttttggctat
     4141 gacgacctct atcgcttaac cagcgccacg ggtacgtacc aacaaacacc gtggacaacg
     4201 aaccgcttca cgctgtcgat gcagtacgat tcgattcaca acatcacaca gaaaaaccaa
     4261 ctgaacgaat ttgtaaaccc gggcggtgcg caccgttatt tcgacacgag ctacaattat
     4321 gcgtacgcgt ataacgccac caaaccgcac gcagcgagtg acatcggccc ttatgaaaac
     4381 ctgtacgacg cgaacggcaa cctaatccgc cgcgaacaca agaacacgca ctggatgcgc
     4441 cacatggtgt gggacgaaga gaaccgcctc gcctgcgtac gcgaaccgtg gcacggctac
     4501 atgccacaac acccgaacag ttgcttcggc ttcggcccgc gtttcgtgta caacgcagca
     4561 ggcacgcgtg tactcaaaga cggttttcaa cgaagtttct ctatcaacga acact
//
LOCUS       DAOMPD010000187         4615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7826_length_4615_cov_2.245833, whole genome shotgun sequence.
ACCESSION   DAOMPD010000187 DAOMPD010000000
VERSION     DAOMPD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7826_length_4615_cov_2.245833"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="PLY93_07580"
     CDS             <1..1399
                     /locus_tag="PLY93_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69375.1"
                     /translation="INHASGNIGGAMASGAVANFTTTQTGVISAIELTGYGSGCIAAP
                     TVTITGGGGSGATAVAIVDSGAQQISRVQITNGGVGYTSAPTVSFSGGCTLSPTGAAR
                     FNRVGQVNIANPGSGYERNPTCTLGGLSAKCVVAGELSNTSLVNYYGGTGYTTGDVCP
                     IVGAGGSGTTCTVTAVGGNLTGCSAISATGGNYSVGRIVKIGGRAEAVVTVSVPAGNV
                     VGITVTNSGCGYSVVPNIEVVGCTTAPTFNVTIPGGRVTATVASAGAGCPVGGKVVIG
                     ENPYVGYADGASAVVNELATDTGHLTFVSVSESAVNTAQLMQLLDRDATGVTSLTRGF
                     SVAYNSVSTTGGISMREAMVRLIHHGVTPSLASATAYFNASLGAAGPAGGVVGNYIRD
                     WPGVGPRYLAGNILNNLNGVTATKSLIDMVNTNTIDLVDTVILLGCGDRVTYSNGATA
                     FSWQQLCTELGPGVW"
     gene            1483..1782
                     /locus_tag="PLY93_07585"
     CDS             1483..1782
                     /locus_tag="PLY93_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRP69376.1"
                     /translation="MDQKQLKEELAALKAQEKKVRAAVGKEKNPAEEAIRSPNGIRYT
                     WLGLEFATTFLVFVWGGSWLDGKWGVSPWLTLGGLLVGFSIAIYRLITVARTLSA"
     gene            1779..2930
                     /locus_tag="PLY93_07590"
     CDS             1779..2930
                     /locus_tag="PLY93_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69377.1"
                     /translation="MSRNIVVFAPAKINLGLRITGRYASGYHRLEGAFLSVSLFDRVE
                     IRKAAQMSVRYVWPTTLPVGARRSALLGIQKAPLLWKSIAMVRDYIVNNAEGITISNN
                     LINEGIVIPPIEIIVNKGVPSPSGLGGASSDAAVLIATLWKIATGNAPLPSALIDASE
                     NLGADIPFFLRYGLYGKAAYLDGVGHELTEVIVCGHFDKLSDREKMLSVRESVYTQNS
                     VVEPVEMPESMSRRLSGFLCVPSFGFPTAQMFATVRTWPLPLVQPSPHAVDSYPSNSK
                     RALRLNEIPYSDEPLTGIRLLQNDFEKAAAVVFPQAHAALQRAQRFMAYTVRQRCGGL
                     WWGGMSGSGAGLYMVTDNDLSPSGMSWIRNVLQVRLGRTWRVFSIYAGL"
     gene            3036..3107
                     /locus_tag="PLY93_07595"
     tRNA            3036..3107
                     /locus_tag="PLY93_07595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3068..3070,aa:Gln,seq:ttg)
     gene            3150..3785
                     /locus_tag="PLY93_07600"
     CDS             3150..3785
                     /locus_tag="PLY93_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRP69378.1"
                     /translation="MTEVKRPSALKRQYVIDFKFQSKFILKAVLPLMLFVLVLAFGFL
                     FAVNSIAKEFQFESTAELIQKLSTTLGQDATSGIIFQKVKLYGLLTLAALALVMVLSL
                     TILFVLFSHKIAGPILRIERTFEEVLGGDLTHRITLRHGDELKETADHLNTMLDGLEA
                     RIKRIHQMSHYMQENLQQMIQEAPADKKDALVKVNDLAKGVEESIIDFKMR"
     assembly_gap    4005..4014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4015..4487
                     /locus_tag="PLY93_07605"
     CDS             <4015..4487
                     /locus_tag="PLY93_07605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP69379.1"
                     /translation="AFRPTICADAVTLPLERESLCALVAMNASIHYLCSTEELLQHFN
                     ECARVLDTQGVYIFDVCPVERACALSGRTQYALSGSVKFAHEFKSPFLTTWVFLKNEG
                     KEIVEEHRQRIFSVDEILTAIQSSAFLLEGFEENYGLLPQNNAAPFMAYVLHKV"
BASE COUNT         1099 a         1043 c         1261 g         1202 t
ORIGIN      
        1 aatcaaccat gcgtcgggca atatcggtgg ggctatggcc tctggggctg tagctaattt
       61 taccactacc caaacgggcg taattagcgc gattgaactc acggggtatg gttcgggctg
      121 tatagcggca cccacggtaa caatcaccgg tggcggtggc tcaggcgcaa ccgcagtggc
      181 gattgtagat tcaggagcgc agcagataag tcgtgttcaa atcaccaacg gtggtgtggg
      241 gtacaccagc gcacccactg tctctttttc aggaggatgt acgctttcac ccaccggcgc
      301 cgctcgcttc aatcgagtgg ggcaggtaaa catcgccaat cccggttcgg ggtacgagag
      361 aaatcccact tgcactttgg gtggtttgag cgcaaagtgt gttgtggcag gagagctttc
      421 aaatacaagt ttagtgaact attatggcgg cacgggctac accacggggg atgtgtgccc
      481 gattgtcggt gcaggcggta gtggaactac ttgtactgtt acggctgtgg gtggaaattt
      541 aaccggttgt tcggcaattt cagcgacggg gggtaactat tccgtgggtc gtattgtgaa
      601 aattggcggt agggcagaag cggtggtgac cgtctcggtg cccgccggca atgtggtagg
      661 aataacagtc acgaactcgg gttgtggtta tagcgtggtt cccaatatcg aagttgtcgg
      721 ttgcaccaca gcgcctacgt ttaacgtcac aatccccggc gggcgtgtga ctgccacagt
      781 ggcgtcagct ggagcgggat gtcccgttgg aggcaaagtc gtcattggtg aaaatccgta
      841 tgtcggctat gccgacggtg cgtctgctgt ggtgaacgaa ctcgcgaccg acaccggcca
      901 tctcacgttt gtgagtgtct ccgaatcagc agtcaacacc gcgcaactca tgcaacttct
      961 cgaccgcgac gccacagggg taactagtct cacccgtggt ttttctgttg cgtacaacag
     1021 cgtatccact acaggcggta tttcaatgcg tgaagcgatg gtgcgtctta tacaccatgg
     1081 ggtaactcct agtctcgcga gcgcaacagc gtacttcaac gcttcgctgg gtgcagcagg
     1141 acctgcaggt ggcgttgtgg gtaactatat acgcgactgg ccgggcgtcg ggccgcgtta
     1201 cttggcgggc aatattctca acaatttaaa cggtgtcact gcgaccaaat cgctcatcga
     1261 catggtgaac accaacacga tcgatttagt cgatacagtg attcttttgg gctgcggcga
     1321 ccgagtcacg tactcgaatg gagcgactgc gtttagttgg caacaattat gtacggaatt
     1381 aggtcctggg gtgtggtaaa taaagtatgt tggactctct atcgtaatct ggagtaacta
     1441 tgtttcattt gacgcacaat gttgaaaaaa atattgatgg gcgtggatca aaagcagctc
     1501 aaagaagaac tggctgcgct caaagcgcaa gaaaaaaaag tacgcgctgc agtcggtaaa
     1561 gaaaagaatc ccgctgagga agcgatacgt agcccaaatg gcatacgtta tacatggttg
     1621 gggcttgagt tcgcgacgac gtttcttgtt tttgtttggg gtggctcgtg gctcgatggc
     1681 aagtggggcg tttcgccttg gctgacactc ggtggtcttc tcgtggggtt ttccatagct
     1741 atctatcgtc tgatcacggt cgcccgaaca ctgagcgcat gagtaggaac atagtcgttt
     1801 tcgcccctgc taaaattaac ttggggctac ggatcaccgg tcgttatgcg agcggttacc
     1861 accgtctgga gggcgcgttc ttgtcggtga gtcttttcga ccgcgttgaa atccgtaaag
     1921 cggcgcagat gtctgttcgg tatgtttggc cgactacttt gccggtgggg gcacggcgta
     1981 gcgcactttt ggggattcaa aaggcccctt tgttatggaa atccatcgct atggtgcgcg
     2041 attacatagt taataacgcc gaggggataa ctatctctaa taacttaatt aatgaaggga
     2101 tagttatccc accaatagaa attattgtaa acaaaggtgt accgtctcct tctggtttgg
     2161 ggggggcatc gagtgacgcg gcggtgttga ttgccacact ctggaaaatt gccacgggga
     2221 acgcccccct cccttcggcg ctcatcgacg caagcgagaa tttgggggcg gatataccat
     2281 ttttcttgcg ttatgggctc tacggcaaag cggcttatct cgacggcgtg gggcatgagt
     2341 tgacagaggt cattgtttgt ggtcacttcg ataagctcag tgaccgcgaa aagatgctca
     2401 gtgttcgaga aagcgtgtat acacaaaact cggtcgttga gcctgtcgaa atgcccgagt
     2461 ctatgtctcg taggttgagt ggatttcttt gtgtgccgag ttttggcttt cccacagcgc
     2521 aaatgtttgc cacagtacgt acatggccgt tgccgcttgt ccagccttcc ccccacgctg
     2581 tggatagtta tccctcaaac tctaaaagag ctttacgcct caacgaaatt ccttattcgg
     2641 acgaacccct tactggtatt cggctattgc agaacgactt tgaaaaagcc gctgctgtcg
     2701 tttttcctca ggctcatgct gctttgcagc gtgcgcaacg ctttatggcg tatacggtgc
     2761 ggcagcgttg tggtggtctc tggtgggggg ggatgtccgg ttctggagca ggactctata
     2821 tggtgacaga taatgatttg tcaccgagtg gaatgtcgtg gatacgtaat gtgctccaag
     2881 tgcggttagg ccgtacatgg cgtgtctttt ctatctatgc gggactgtaa aaaatgtcct
     2941 ttgggatgtc taaaaagggc gaatttgacg tttgttttca actttttaga ctgtctaatg
     3001 gttgcgtcag cagtcgatta ggcaacgcat aaaattgggc cgtagccaag tggtaaggca
     3061 gtggattttg attccaccat gcgaaggttc gaacccttcc ggcccagcaa aactcttgac
     3121 gggggaagca atccccccga ccaagaaaaa tgaccgaagt aaaaaggccc tctgcactca
     3181 aacgacagta cgtcatcgat tttaaatttc aaagtaagtt tattcttaaa gcggtgttgc
     3241 cgttgatgct ttttgttcta gtgctcgcct tcggttttct ttttgccgta aatagcatcg
     3301 ccaaagaatt tcaatttgaa agcaccgccg agttaatcca aaaactttcg acaacgttgg
     3361 ggcaagacgc cacatcgggt atcattttcc aaaaagtaaa attgtacgga ttgctcacgt
     3421 tggcagcgtt ggcgttggtg atggtgttat cgctcacgat tctttttgtc cttttttcgc
     3481 ataaaattgc aggtcccatt ttacgcatcg aacgcacatt tgaagaagtg ttggggggcg
     3541 atttaacaca ccgtatcacg ctacgccatg gcgacgaact caaagagacc gccgatcact
     3601 tgaacactat gctcgatggc ttggaagcgc gcattaagcg catccaccaa atgtcgcatt
     3661 acatgcaaga aaaccttcaa caaatgatcc aagaggcgcc ggcagacaag aaagatgcgc
     3721 tcgtaaaagt caacgatctg gcgaagggcg ttgaagagtc gattatcgac ttcaaaatgc
     3781 gttgatttta aaaagtagtg caagcgtccc gccgtacaca gcgtttagtc cctactatgc
     3841 gcaggttatg gaggatatac cctacagcgc gtggttttct ttcattaaaa agtttttacc
     3901 tgcaccgtct ttagtaagta aacccattgt ggaaattttt tccggtccgg ggaagatggc
     3961 gcattttttg aaggatgcgg gatggcgggc gtacacactc gattnnnnnn nnnnctgcgt
     4021 ttcgacccac catttgtgcg gacgcggtaa cattaccatt ggagagggaa tcgctttgtg
     4081 cgctcgttgc aatgaatgcg tcgattcatt atttatgtag tacagaagag cttctccaac
     4141 atttcaacga gtgcgcgcgt gtgctcgata cgcaaggagt gtatattttt gacgtatgcc
     4201 ctgtcgagcg cgcgtgtgcg ctttcagggc gcacacagta tgcactttct gggagtgtaa
     4261 aatttgcgca tgagtttaaa tctccttttt taacgacatg ggtttttctt aaaaacgaag
     4321 gaaaagaaat cgtcgaagag catcgacaac ggatattcag cgtagacgaa attttaacgg
     4381 caatacaaag ttctgcgttt cttcttgagg gatttgaaga aaattacggg cttcttccgc
     4441 aaaacaacgc tgcacctttt atggcgtatg ttttacataa agtttaagta atacccacga
     4501 aagaaaaaac gccaacgcgg gcaatcctac aaaaagaaaa gtgatggtgt gcgagacgtc
     4561 cgtctcgcgt gtttgagggt tgtagcgtgc aaggctcaac gcgatgccga tagca
//
LOCUS       DAOMPD010000188         4588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7874_length_4588_cov_3.279506, whole genome shotgun sequence.
ACCESSION   DAOMPD010000188 DAOMPD010000000
VERSION     DAOMPD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7874_length_4588_cov_3.279506"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PLY93_07610"
     CDS             <1..1292
                     /locus_tag="PLY93_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69380.1"
                     /translation="GVLFLGGAIPFIETTAGAVDFPDSVLYLKSDALAHFLLKNRSLL
                     KTYLAKAAARYTLVCDIPQTYIAHVTSEEAGMEAANFAVFLAAQFSVNIGKTLLVDAD
                     PQNQSLFSLLTFEEKPPVLTENIQTTVTFKSDMTKAILPLAKNFFYLNLQAPSLRPFN
                     DDELCRIIPLLDGDFENIVIYAGKMESAWLSKNAHINYAICSKAYISEAAQLLRHQGG
                     FHTVLLKKGSEAYLPNLNAQFAQKQSLATWIESDSKQTALRTFVLRLRTAKNLVMGRE
                     SDDTNTLHCHVGIDLFAHYTKGTIKSLEGALRQLQQKLTPQYPKKSFFRPASIEHALR
                     NIPTRTSTTLLLLDDAPQLVSLVDSSKLKAAAILPAGVYPALSYQKMRIASCTAGGLT
                     HFQEIIARGGFNSLQKAERLVLKNPSALGAILEQLQP"
     gene            complement(1352..2230)
                     /locus_tag="PLY93_07615"
     CDS             complement(1352..2230)
                     /locus_tag="PLY93_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRP69381.1"
                     /translation="MDSKAMEKILIAGGGAMGAGIGYVFARAYPNAQVDVIDTNEKIR
                     TQAAECFQKWAKKDQEKDREAGKTPVVVDERLNLLGGFGKTQLQYDFMLEAVTENLEV
                     KKKIFAEFDLRAHDASIFASNTSSLSITELSTAVNPKRQGRVLGMHFFNPPRVMKLLE
                     IITTETTDAETLQAAKDIAVQLEKEPVICKDAPGFITSRLGIVLINEAIFALQEGLMS
                     AEDIDTSMKLGYNFPMGPLALADFIGLDVVFAITQSLYQNYQDSKYRPCMLLRKKVEA
                     GHLGQKTGRGFFTYKN"
     gene            complement(2285..3271)
                     /gene="mnmH"
                     /locus_tag="PLY93_07620"
     CDS             complement(2285..3271)
                     /gene="mnmH"
                     /locus_tag="PLY93_07620"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRP69382.1"
                     /translation="MALPQSLSIAKFLALKIPYLDVRSEGEFTLGHIPHAQSMPLFNN
                     IERAQVGTLYKQTSKEAAILKGFELVGPRLRAMAEQGLAAAHDNRIGVYCWRGGMRSA
                     SVAWLLESLGLEVYTLKGGYKAYRNFALAQFNEPRPLCVIGGKTGSRKTQLLGVLEKE
                     GKNVIDLERLANHRGSAFGYADDYAQPTQEQFENDLAWVLFHLTPDETVFIEDESRLI
                     GHVHIPDMLWSQLRRAHVVVLNWPLEKRVEFLTREYHASSTVMEKNIEAIKKRLGGDR
                     YKEALALLAANDRAGVCRIMLDYYDRAYEYGLSKREQSTIRYVEGAEAFKIL"
     gene            complement(3275..4213)
                     /locus_tag="PLY93_07625"
     CDS             complement(3275..4213)
                     /locus_tag="PLY93_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRP69383.1"
                     /translation="MRLCFSFLFLTATIFPWGMRGHRIVARIAENHLTDTAREKIQTL
                     LKGEPLTRVANIPDHVRSNNKWQCAAPLHYVSVDEKKEYVASKRNDNGDSVQALVYFE
                     DVLRSRGRSPLEKRNAVIWMTHLVGDMHQPLHVGRACDRGGNTVVVTWFGKRSNLHKV
                     WDSEFVNHEELSFSEYASEIDNSKEEERSQWAKATYFDWMDDARTGQAQIYTCYAKDG
                     CCSEKKNGKCVLIETLFNSCGKDTAPVVLQYAYVAKNRALLNAQLLKGGVRLAALFNR
                     IFDDTPLSPDELALRETIKNTSTTKKIDACVIHALH"
     gene            complement(4197..>4588)
                     /locus_tag="PLY93_07630"
     CDS             complement(4197..>4588)
                     /locus_tag="PLY93_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRP69384.1"
                     /translation="LQQEAKDTPILIHCFTGNKAEMEDFLSLGAFISFSGIVTFKNAA
                     AVQEAAAACPQERILVETDAPFLAPVPFRGKVNQPGYVKQTLDFLAALRSESVDVLAA
                     ACTANTQQFYRLHQTGHKTNINDAPLL"
BASE COUNT         1180 a         1130 c         1037 g         1241 t
ORIGIN      
        1 tcggtgttct ttttttagga ggggcaattc cttttattga aacaacggct ggtgcggtag
       61 attttcccga tagtgttctc tacttaaaga gcgacgcatt agcacacttt cttttgaaaa
      121 atcgatcctt attaaaaact tatttagcga aagccgccgc gcgctacaca cttgtgtgtg
      181 acatccccca aacgtatata gcgcacgtta caagcgaaga agcgggtatg gaagcggcaa
      241 actttgcagt gtttttagca gcgcaatttt ctgtaaatat aggcaaaaca ttgctcgtgg
      301 atgccgaccc acaaaaccag tctctttttt cactacttac ttttgaagag aagccgcccg
      361 tgcttaccga gaatatacag acaaccgtaa cattcaagag cgatatgaca aaggcaattt
      421 taccgctcgc aaaaaatttt ttttatttga acttgcaagc acccagtcta cgcccgttta
      481 acgacgatga actttgtcgc attataccgc tcctggatgg tgactttgaa aacatcgtta
      541 tatacgctgg taaaatggag agcgcgtggc tttccaaaaa tgcacacata aattacgcta
      601 tttgctcaaa ggcatacatc agcgaagcgg cgcagctttt acgccaccaa ggcgggtttc
      661 atacagtgct gctcaaaaaa gggagcgaag catacttacc caacctcaat gcacagtttg
      721 cacaaaaaca atcgcttgca acatggatag agagtgatag taagcaaacg gcattgcgta
      781 catttgtgct gcgccttcgt acggcaaaaa atcttgtcat gggacgcgag agtgacgaca
      841 ccaatacttt gcactgccac gttgggattg atttattcgc gcactacacc aagggtacaa
      901 taaagagttt agagggcgcg ttgcgacagt tacaacaaaa actaacgccg caatacccaa
      961 agaaaagttt tttccgccct gcttcgattg agcacgcgct aaggaatata cccacgcgta
     1021 catccacgac gctgttgttg ctcgacgatg cacctcagct tgtaagtctc gttgatagca
     1081 gcaagcttaa agcggcggca attttacctg cgggggttta tcctgcgctc tcgtatcaaa
     1141 aaatgcgtat cgcttcttgt accgctgggg gactaaccca ctttcaagaa attattgcac
     1201 gaggcggatt taactcttta caaaaagcag aacgtttggt gttaaaaaat ccctctgcat
     1261 taggggcaat attggaacag ttgcaacctt gatctctttg taaaaaatac cacggcaatg
     1321 tgagtggata aaacaacgcc aaaagcaagc attaattctt atacgtaaaa aatccgcgcc
     1381 ctgttttttg gcccaagtga cctgcttcga ctttttttct caaaagcata cacgggcggt
     1441 attttgagtc ttggtagttt tggtaaagcg attgcgtgat ggcaaagacc acgtcgagtc
     1501 cgataaaatc ggccaacgca agaggtccca tggggaagtt ataccccaat ttcatcgagg
     1561 tatcgatgtc ctccgccgac atgagtcctt cctggagagc aaaaatggct tcgttgatga
     1621 gaacgatccc caaacgcgac gtgataaacc ctggtgcgtc tttacagata accggttctt
     1681 tttctaattg cacggcgata tcttttgccg cttgtaatgt ttctgcatcg gtggtttctg
     1741 tggtgatgat ttctaaaagt ttcatcacac gaggtgggtt gaaaaagtgc atccccaaaa
     1801 cgcgtccttg gcgtttggga ttcaccgctg tggaaagttc ggtgatagaa agcgacgaag
     1861 tattactcgc aaaaatagac gcatcgtgag cgcgtaaatc aaactcggca aaaattttct
     1921 ttttgacttc taagttttct gtaacggctt caagcatgaa atcgtattgc agttgcgttt
     1981 taccaaaacc tcccaacaag ttgagtcgtt cgtcaacaac aacgggtgtc ttacccgctt
     2041 cacgatcttt ttcttgatct tttttcgccc acttttggaa acactcggcg gcttgtgtgc
     2101 gtattttttc atttgtgtcg atgacatcaa cctgtgcgtt ggggtatgca cgcgcaaaaa
     2161 cataaccaat gccagcgccc atcgcgcctc cgccggcaat caaaattttt tccatggctt
     2221 ttgagtccat aggtaaaaag tgaaatagga tacaaggcga ctattaaaga agcactctgg
     2281 aattttacaa tatcttgaac gcctctgcgc cctcaacgta ccgaattgtg ctctgttctc
     2341 gcttggaaag cccgtactcg tatgcgcggt cgtaataatc gagcataatg cggcaaacac
     2401 ccgcgcggtc gtttgctgca aggagagcca acgcttcttt atagcggtcg ccacccaaac
     2461 gcttttttat tgcttcgata tttttttcca tgactgtaga gctcgcgtgg tattcgcgcg
     2521 ttaaaaactc gacgcgtttt tctaatggcc agttgagaac caccacatgc gcgcggcgca
     2581 gttgactcca taacatgtct gggatatgca catgcccgat gaggcggctt tcgtcttcga
     2641 tgaaaacggt ttcatcgggg gttaaatgga agagcaccca cgctaaatcg ttttcaaact
     2701 gttcttgcgt cggttgcgca taatcatcgg cgtacccaaa cgcagaacca cggtggttcg
     2761 ccaagcgttc caaatcgatg acattcttgc cttctttttc taaaacgccg agaagctgcg
     2821 ttttacgcga accggtttta ccgccgataa cgcacagggg gcgcggttca ttaaactgcg
     2881 ctaacgcaaa attccggtac gccttatacc ccccttttaa cgtatagact tcaagaccca
     2941 gactttcaag tagccacgct accgacgccg agcgcatgcc accacgccaa caataaacac
     3001 cgatgcggtt atcgtgcgca gcagccaagc cttgttcagc catcgcgcgt aggcgtggcc
     3061 ccaccaactc gaaccctttc aatatggctg cttctttact cgtttgttta tagagagtgc
     3121 caacctgtgc gcgctcgata ttgttaaaaa gcggcatcga ttgtgcatgc ggtatatggc
     3181 ctaaagtaaa ctcaccttcg gaacgcacgt cgaggtatgg aattttgagc gctaaaaatt
     3241 tggcgataga taaactttgt ggcaacgcca tttcctaatg tagcgcatgg atgacacacg
     3301 catcgatttt ttttgttgtg cttgtattct ttattgtttc acgcaacgca agttcatcgg
     3361 gtgaaagtgg cgtgtcatca aaaatacgat tgaaaagagc cgccagacgc actccccctt
     3421 tcaaaagctg tgcgtttagg agcgcacggt tcttcgccac gtacgcgtat tgcaacacca
     3481 ccggcgctgt atcttttccg caagaattaa aaagtgtttc tatcaaaaca cacttgccgt
     3541 tttttttctc gctgcaacaa ccatcttttg cgtagcaagt gtaaatctgc gcttgacctg
     3601 tcctggcgtc gtccatccag tcgaaatacg tcgctttcgc ccactggcta cgttcttctt
     3661 ctttgctatt gtcaatttcg cttgcgtatt ctgaaaaact caactcttcg tggttgacaa
     3721 actccgaatc ccaaactttg tgcagatttg aacgtttgcc aaaccacgtt accacaactg
     3781 tgttaccgcc gcgatcgcac gcgcgcccca catgcagcgg ttggtgcatg tcgcccacga
     3841 ggtgcgtcat ccaaatgacg gcgtttcttt tttctaaagg agatctaccc cgactgcgca
     3901 aaacatcttc aaaatacacg agcgcttgta cgctgtcccc gttatcgttt ctttttgaag
     3961 cgacatactc ttttttttca tcgacgctca catagtgcaa cggtgcggcg cattgccact
     4021 tgttgttcga gcgtacgtgg tcggggatgt tcgcgacacg ggtgagcggt tcgcctttga
     4081 ggagtgtttg gattttttcg cgtgccgtgt cggttaaatg gttttcagca atgcgtgcga
     4141 caatacgatg gccacgcata ccccatggaa aaattgttgc ggtgaggaat aaaaaactaa
     4201 agcaaaggcg catcgttgat gttggttttg tggccagttt ggtgtaaacg ataaaactgt
     4261 tgtgtgtttg ccgtacacgc tgccgctaac acatccaccg attcgctacg caacgccgct
     4321 aaaaaatcca gcgtttgttt cacataccca ggttgattca ctttgccacg gaatgggacc
     4381 ggcgccaaga aaggcgcgtc ggtctccacg agaatacgct cctgtggaca cgccgccgct
     4441 gcttcttgca ccgccgctgc gttcttgaaa gtcacaatcc ccgaaaaaga aataaatgcc
     4501 cccaaagaca aaaaatcttc catctcggct ttgttgccgg taaagcagtg gatcagaatg
     4561 ggagtgtctt ttgcttcttg ttgtaaga
//
LOCUS       DAOMPD010000189         4573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7899_length_4573_cov_3.181054, whole genome shotgun sequence.
ACCESSION   DAOMPD010000189 DAOMPD010000000
VERSION     DAOMPD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_4573_cov_3.181054"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLY93_07635"
     CDS             complement(<1..500)
                     /locus_tag="PLY93_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69385.1"
                     /translation="MGSEFLYTKLELFTVTLSGGLMRAAALTICAPLFPLLAAVPPTA
                     SGRDSLRNELTRQVPECSLVAENGARLLATGVNEADVLAEWRNICPNNDFIHQYELSG
                     RLATIDKTSPDISWNLWMTIRGRKDHRRLPPADGEYYRLLAKRAALANPTSIDGKLLK
                     TYFLSG"
     gene            complement(736..1017)
                     /locus_tag="PLY93_07640"
     CDS             complement(736..1017)
                     /locus_tag="PLY93_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69386.1"
                     /translation="MGYYKVSAVDFSLLYGISAAGAMLGSFLNGQLVRRFAPKKILRS
                     PRSWIMSHQGVTIPTRVGEPVRQLAEAIFLQVFIRYFAQIPPPKRRATP"
     gene            1088..2380
                     /locus_tag="PLY93_07645"
     CDS             1088..2380
                     /locus_tag="PLY93_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRP69387.1"
                     /translation="MPKIKEAGMRHIRAKSIFPGRVRGVMAEFKKERFAAFQKEAQNP
                     RIEYQNFKSALRQAQTQLITEKKMMLDKKRQTPHGITTLDDAIGVLESHILTLEDPYF
                     LGEIKKLIFTTGATAAAALGAVISTISRQLSFDTVNHVEGKNNRHFVHDMNDMKHRIL
                     HFLGVQHHFTLPKGVTIVAAAKLTVSEVLALKRSGVRGIVIGNMSDAAHEIVMLRAMR
                     IPCVAVSDLRFFRLRKQIPVIIDADIGYIVLRPRKSMRFIETPIDTTPPWSQNITLPS
                     GEKISLSAPLHFLGELNNFSADTRLVKKIGIYRTEYQISEAGELLSTKQLTKQYESLF
                     TRFPKTEITFRLFDFSEDKNFVKNPRIEKNHDLRGIRFLIAEKKILESQLNALIKAAE
                     NTRHNLRILIPYLSEMHELHAIWDILQGILPQKKKFFF"
     gene            2391..2825
                     /locus_tag="PLY93_07650"
     CDS             2391..2825
                     /locus_tag="PLY93_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69388.1"
                     /translation="MIETPTAVFAAKTWTPFVDFFYVGTSDLLSALSAYQRNQQEALQ
                     RTLLSDASCTVFSFLKQLMQKSKVTVCGEIVGEPWAIAYLRHFGFQNFVVPAHRIGTT
                     ANFLQMLTKEACATYTRKIFKIKDEVNRLESARRMILDILYG"
     gene            2855..3799
                     /gene="pip"
                     /locus_tag="PLY93_07655"
     CDS             2855..3799
                     /gene="pip"
                     /locus_tag="PLY93_07655"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRP69389.1"
                     /translation="MRELYPEIHKVKSYTLRVSSLHTLYIEECGNPRGTPILFLHGGP
                     GAGLSPNHRRFFNPQKYRIILFDQRGAGKSKPYAELRENTLWDLIDDIEKIRRKLKIT
                     KWVVFGGSWGSTLALCYAINYPEKVLHLIVRGIFLCRREEIQWFYQGGAHQIFPDAWK
                     NYIRPIPKSERHDLLSAFYLRLTSKDKKVWLEAARAWSAWEGATICLQSDPKVVHEFN
                     EIAISLARIECHYFMHNCFFPEDNYILNNVYKLSKISTEIIHGRYDVVCPVKNAFDLK
                     EKMPHARLRVIPNFGHAASEPGIRSALIEATDRYIAKK"
     gene            4060..4422
                     /locus_tag="PLY93_07660"
     CDS             4060..4422
                     /locus_tag="PLY93_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69390.1"
                     /translation="MLQKGNTSSALLFFPFEGKVQKTEIRIEPFQWDYVTVKIQNHPQ
                     AFLHKVRAWAEQSILMENSKERLKNAVHSLVALKESASELNLLLDLGSAEVGTFAELL
                     TIAIQENAKRIDIVSASN"
BASE COUNT         1328 a         1096 c         1001 g         1148 t
ORIGIN      
        1 ctgcccgaga ggaaataagt ctttagcagc ttgccgtcga tcgaggtggg gttagcaagt
       61 gctgcacgtt ttgcgagcaa ccggtaatat tccccgtcag cgggtggcag gcgccggtgg
      121 tcttttctgc cacgtatcgt catccacagg ttccagctga tgtcaggcga ggttttatca
      181 atcgtggcca ggcggccgga aagctcatat tggtgtataa agtcgttatt gggacagatg
      241 ttccgccatt cggccagaac atcggcttcg ttcactccag tcgctaagag ccgcgccccg
      301 ttttcagcaa ccagagagca ttcgggcacc tgccgtgtca gctcgttacg cagcgagtcg
      361 cgccccgatg ccgtgggggg cacagccgcc aatagaggaa agagcggcgc acaaatagta
      421 agggctgcgg cgcgcatcag gccaccgctt agcgtcaccg tgaacagttc gagtttagta
      481 tataagaact cactccccac tcacgtctac tgaacccaag ctctccaggt gtaaagcctg
      541 tttcacagag tgacgagtta tgagcctgtg aggaactgtg cgccaactcg cgcagtgttt
      601 attgaggggg caattttttt gcgaattttt gtacgcggtt cgctcaatat tgcgaaattt
      661 tgcacaaatt cgggcgtctt cacctcgcgc aaagcgaatt ggcacaattt gccgtcggcc
      721 aaagcgccgc cggcctcacg gcgttgcacg ccgctttggc ggaggaattt gtgcaaaata
      781 gcgtataaaa acttgcaaaa aaattgcttc agcgagttgg cgcacaggct ctccaactct
      841 cgtagggata gttaccccct gatgcgacat aatccaagaa cgaggcgagc ggaggatttt
      901 cttcggcgcg aagcgccgca caagttgccc gttcaagaaa ctgccgagca tcgccccagc
      961 ggcactgatg ccatagagca aggagaaatc caccgcactg actttatagt agcccatgaa
     1021 aatgagcgac ttgggcgcac aggctctctg tagcttacac ctttaaaaag caagttcata
     1081 atccaatatg ccgaaaatta aagaggcggg catgcgtcat attcgggcaa aaagtatttt
     1141 tccaggccgt gtgcgcggcg tcatggctga atttaaaaaa gagcgctttg ctgcattcca
     1201 aaaagaagca caaaatccgc gtatcgaata ccaaaatttc aaaagcgcct tgcgccaagc
     1261 gcaaacacag ctgattacag aaaaaaaaat gatgctcgac aaaaagcgcc aaacgccgca
     1321 cggcatcacc acattggatg atgccattgg agttctcgaa tcgcatatac ttactttaga
     1381 agacccttat ttccttggcg aaataaaaaa attaattttt actacaggag cgaccgccgc
     1441 tgctgcacta ggcgcggtaa ttagcactat ctcgagacaa ctttcgttcg atacggtaaa
     1501 ccatgttgag ggtaaaaata accgtcattt tgtgcacgac atgaacgaca tgaaacaccg
     1561 tatcctccat tttcttggag tgcaacacca ttttacatta cccaaaggag ttactatcgt
     1621 tgcagcggca aaacttaccg taagcgaagt acttgccctt aagcgctctg gcgtacgcgg
     1681 tattgtcatt ggaaatatga gcgatgctgc acacgaaata gtgatgctgc gtgcaatgcg
     1741 aattccctgc gtcgccgtaa gcgaccttcg ctttttccgt ttacgcaaac agatccccgt
     1801 tattatcgat gcggatatag gctacattgt tttacgccct cgaaaatcta tgcgtttcat
     1861 tgaaactcct atagatacaa ctccaccttg gtcgcaaaac attacgctac cttctggaga
     1921 aaaaatatcc ctatctgctc cactacattt tttaggtgaa ctcaataatt tttctgcgga
     1981 tacgcgttta gtaaagaaaa ttggtattta tagaacggaa taccaaattt ccgaagctgg
     2041 agagttgctc tcaacgaagc aacttactaa acaatacgaa agcttattta cccgttttcc
     2101 taaaacagag ataacgtttc gcttatttga tttttctgaa gataaaaatt tcgtaaaaaa
     2161 tccacgtatc gaaaaaaatc atgacttacg aggaatccgc tttctaatcg cagaaaaaaa
     2221 aattttagaa agccaactta acgcgcttat taaagcggca gaaaatacac gccacaattt
     2281 acggatactt atcccttacc tttctgaaat gcatgaactc catgccattt gggatattct
     2341 ccagggcatt ttaccacaga aaaaaaaatt tttcttttga attaggagcg atgattgaaa
     2401 cgcctacagc cgtttttgca gcaaaaacgt ggacgccatt cgtcgatttt ttttacgtag
     2461 gtacatccga tttacttagc gccctctcgg cgtaccagag aaaccaacaa gaggcgttgc
     2521 aacgtaccct attgagcgat gcaagttgta ctgtatttag ttttcttaaa caacttatgc
     2581 agaaaagcaa agtcaccgta tgcggagaga ttgtgggaga accttgggcc attgcatacc
     2641 ttcggcattt tggttttcaa aactttgtcg tacctgcaca ccgcataggt acaactgcaa
     2701 actttttgca gatgctcaca aaagaagcgt gcgcgacata cacgcgaaaa atttttaaaa
     2761 ttaaggacga agttaatcgc ctcgagagtg cacggcgaat gattctggat attctttacg
     2821 gataatcgta agaacccact ataggtataa aagcatgcgc gaattatatc cagaaatcca
     2881 taaagtaaaa tcgtacactt tgcgtgtatc ttcactccac accctataca ttgaggaatg
     2941 tggaaatcct cgcggaacac ccatactatt tttacacgga gggccaggcg ctggactttc
     3001 acccaaccac cgccgttttt ttaatcccca aaaataccgt atcatcttgt ttgatcaacg
     3061 tggcgcagga aaaagcaaac cgtatgcaga attacgcgag aacaccctgt gggatctaat
     3121 cgacgacatc gaaaaaattc gccgtaaact taaaattaca aaatgggttg tatttggcgg
     3181 ttcatggggt agtacgcttg cgctttgcta tgcgataaat tatcccgaaa aggtactcca
     3241 ccttatcgtc cgaggaattt ttctttgccg ccgtgaagaa atccagtggt tctaccaagg
     3301 aggggcacac caaatttttc ccgatgcgtg gaaaaattac atccgtccca ttcctaaaag
     3361 tgaacgccac gatttgcttt ccgcttttta cctgcggcta acttcaaaag ataaaaaagt
     3421 atggcttgag gcagcgcgtg catggagcgc ttgggaaggt gcaaccattt gtttgcagag
     3481 cgatccaaaa gtagtccacg aatttaacga aatcgctatt tctttggcgc gtatcgaatg
     3541 ccactatttt atgcacaatt gtttttttcc cgaagacaat tatattttaa acaacgtata
     3601 caagctctca aaaatttcta cagaaattat ccacggcaga tacgatgttg tctgccccgt
     3661 taaaaatgca tttgacctta aagaaaaaat gccacacgca cgccttcggg ttatccccaa
     3721 ttttggccat gcagcttctg aacctggaat ccgttctgca cttatcgagg caacggatag
     3781 gtacattgca aaaaaataaa acggagataa accctcaata ccgtgccctt gcttctgcaa
     3841 gcgcatcgta aagagagcgc caaaaagcat cacgtatatc agtcccattg ggcgcaagtt
     3901 gtgtcataag tattataacc attttttgtc gcgggtccac acggtaaaaa gtcccatacg
     3961 ctcccgtcca gccgtaagaa gcaaccgccc tctcgccatt tgcatttgga taaagcgaaa
     4021 tatcaaaccc atacgtaaaa ctggcaggca tcggcgctcg tgctccaaaa ggggaatacg
     4081 tcatctgctt tactgttttt tccttttgag ggcaaggttc agaaaacaga aattcgaatc
     4141 gaaccatttc aatgggacta tgtcacagtt aaaatacaaa atcatccaca ggccttttta
     4201 cataaagttc gagcatgggc agagcaaagt attctaatgg aaaactccaa agaacgatta
     4261 aaaaacgcag ttcattcatt agttgctctt aaagaatcag ctagcgaatt gaacctatta
     4321 ctggacctgg ggtccgcaga agtcggaacc tttgcagagc tgttaaccat agcaattcaa
     4381 gaaaatgcaa aaagaataga tatagtttct gcgtcgaatt gacaaccgcg tacaacaaaa
     4441 tttacccgct gcgcgcgcga ccactccgtt gggtcgcgtg ctcgggtctg cgcggcaaag
     4501 atcgcggatg tccgggacga gaatgatatg catttcgccc ggccaccgcg agccgctccg
     4561 accacaattt ggc
//
LOCUS       DAOMPD010000190         4565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7914_length_4565_cov_2.305322, whole genome shotgun sequence.
ACCESSION   DAOMPD010000190 DAOMPD010000000
VERSION     DAOMPD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7914_length_4565_cov_2.305322"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1127)
                     /gene="fliM"
                     /locus_tag="PLY93_07665"
     CDS             complement(99..1127)
                     /gene="fliM"
                     /locus_tag="PLY93_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRP69391.1"
                     /translation="MTDVLSQDEIDQLLSAISSGDAQTEDFSATQAEQKKVKIYDFKR
                     PDKFSKDQIRTVQMMHETFARLTTTALSAKLRAMVAVHVQSVDQLTYEEFIRSIPNPT
                     TLSLINMDPLKGSAILEVDPSITFTIIERLFGGGGEHSKLTRELTDIEMSVIEGIIVH
                     ILGNLREAWSTVIDLRPRLGTIETNPQFAQIVPPNEMVMLITLETKIGESEGMMNLCI
                     PYITIEPIIQKLSAQYWFSSIRRGESDVNRTMIQSRLDNVQVQISVEIGTVSLGFRDI
                     MNLKVGNIIKMPKTTVREEFTFKVEERAKFKCRPGRIGSRLAVQIGEAVVDTPDELLI
                     GTRNEEES"
     gene            complement(1191..2201)
                     /locus_tag="PLY93_07670"
     CDS             complement(1191..2201)
                     /locus_tag="PLY93_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69392.1"
                     /translation="MIRGISLYAVSIALSSVMALSPRHQKVDHHGNATENFSARGLRL
                     YASAEKLRFAKNEPIIFTLSLVNEGAYPITIYLHENPLKNFTVIARDPKGKSLNIKEE
                     MYYRGTSDWRDTHFTRYTGETYPMRKMILHVGEKFEKSIRLSDYVEWDTFAADLKKLN
                     VAAYFYPNPLQAERYSIQTAAPLHFLYDQSREVLPEKDNTADASIETPSVSAREIVYL
                     MLSAEFTKNWPQYFKYVSLKDIIRDYPEFARLYADESSQVRAQALGEFRKFLSGRGAH
                     HLLRFRVLSGESSERGDIAEVRVRARRNVEGFERDYLTTYYLTKREALWQVTGVESRL
                     AD"
     gene            2282..3238
                     /gene="pdhA"
                     /locus_tag="PLY93_07675"
     CDS             2282..3238
                     /gene="pdhA"
                     /locus_tag="PLY93_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRP69393.1"
                     /translation="MLKERLFTFYEKMLLIRRFEEAAAKAYATGKIRGFCHLYIGEEP
                     VAVGAIHNLEPHDYVIATYREHGHALARGMSPRMAMAELFGKATGCSKGLGGSMHLFS
                     RELNFMGGHGIVGGHVAVATGHAFASKYRSDHGVTLCFVGEGGTNIGAFYEGLTYASL
                     WNLPVVFIIENNLYAMGTPLYRSQPTHDLTAKAKAFNMPSERIDALSVEKVYQTVKKA
                     VTRARAGLGPTLLEMITYRFRGHSMSDPAKYRPHGELEKMKEKDPMDTTRAKLKRHGF
                     TDARLEELDAKIEAEVEDAVQFAEASPELSYQEMKELVFTTT"
     gene            3508..3882
                     /locus_tag="PLY93_07680"
     CDS             3508..3882
                     /locus_tag="PLY93_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69394.1"
                     /translation="MGKIQRTYVGISGTGKSEFFNGIINQFAGTEAANEIAMPDGIVR
                     NIRFYKYDTTTCSFPITIVLRKNRVDTHVKIVIPAGEMIRPTSIAETVPYNAGDVLVW
                     KASIATSCSCPHRVGVWAVYDY"
     gene            complement(3997..>4565)
                     /locus_tag="PLY93_07685"
     CDS             complement(3997..>4565)
                     /locus_tag="PLY93_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004492708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRP69395.1"
                     /translation="GLAGLTNATRYAILNANYIKARLEKHYPVLYSGSRGRCAHELIL
                     DTRVFKQTTGVEVEDIAKRLMDYGFHAPTVSFPVPGTLMVEPTESEPKAELDRFCDAM
                     IAIREEIRRIADGKSDKADNPLKGAPHTAKMVSADAWTHSYSRHEAAYPLPYVAAQKF
                     WPSVGRVDNVYGDRNLVCSCLPVEDYVA"
BASE COUNT         1142 a         1116 c         1071 g         1236 t
ORIGIN      
        1 gtattcgttc atttggcaac cgtacgtttc aatgtagaat tttttagtta cacccataat
       61 ttcaaaaacc cgttttatga aacagggtga aaactctttt atgattcttc ttcgttacgc
      121 gtaccgatca agagttcgtc cggcgtgtcg acaaccgctt cgccgatttg caccgcaagg
      181 cgtgaaccaa tacgcccagg tcggcattta aatttagccc gctcttcaac tttgaaagta
      241 aactcttcac gcactgtcgt tttaggcatc ttaatgatgt tccctacttt taagttcata
      301 atgtcgcgga agcctaaact caccgtgccg atctctaccg aaatttgcac ttgaacgtta
      361 tctaatcggc tttggatcat cgtacgattt acatcgctct cgccacggcg gatggacgaa
      421 aaccaatact gcgccgagag tttttggatg ataggctcga tggtaatata agggatacaa
      481 agattcatca tcccttcgga ttcgccgatt ttggtttcaa gagtgatgag catcaccatt
      541 tcattcggcg gcacaatttg tgcgaactgt ggattagttt caatggtacc caagcgtggg
      601 cgtaaatcga tcaccgtcga ccaagcttcg cgcaagttac ctaaaatatg tacgataatc
      661 ccttcgatga ccgacatttc aatatcggtg agttcgcgtg taagtttact atgttcgccg
      721 ccaccgccaa aaaggcgctc gatgattgtg aaggtaatcg acgggtctac ctcaagaatc
      781 gccgaacctt taagcgggtc catgttgatg agcgagagcg tcgttgggtt gggtatcgag
      841 cggataaact cttcgtaagt gagctggtct accgactgga cgtgcaccgc caccatcgca
      901 cgcaattttg ccgaaagcgc agtggtggtt aagcgcgcga acgtttcgtg catcatttgc
      961 acggtacgga tttggtcttt tgaaaattta tcggggcgtt tgaagtcgta aattttaact
     1021 tttttctgct ctgcctgcgt tgcagaaaaa tcttcggttt gcgcatcgcc cgacgaaatc
     1081 gcagaaagga gttggtctat ctcgtcttgt gataatacat cggtcatggt ctatcgctcc
     1141 atcgaagatt tattttttcg tgttttttat tttcctgcat atataattat ttaatcggca
     1201 agcctcgact caacccccgt aacttgccaa agagcctctc gtttcgtcaa atagtacgtt
     1261 gttaaataat cgcgttcaaa cccttcgaca ttgcgtcgtg cacgcacgcg cacttcggca
     1321 atgtcgccac gttcgctcga ctcccccgac agaacgcgga atcttaacaa gtggtgcgcg
     1381 ccacgcccgc ttaaaaattt acgaaactca ccgagcgctt gcgcgcgcac ttggctcgat
     1441 tcgtcggcgt agaggcgtgc aaactcgggg tagtcgcgga tgatatcttt gagtgaaaca
     1501 tatttgaagt actgcggcca attttttgta aattctgccg agagcatcaa atacacaatt
     1561 tcacgcgcac tcaccgacgg cgtttcaata cttgcgtcgg ctgtattatc tttttcagga
     1621 agcacctcgc gactttggtc gtacagaaaa tgaagcggcg cagccgtttg gatgctatac
     1681 ctttctgctt gcaacgggtt tgggtaaaaa tacgcagcga cgtttaactt ttttagatcg
     1741 gcggcaaacg tatcccactc aacataatcg cttaaacgga tggacttctc aaatttttca
     1801 ccgacgtgca gaatcatttt gcgcattgga taagtttcgc ccgtgtatcg tgtaaagtgt
     1861 gtatcgcgcc agtcggatgt ccctcggtaa tacatttctt cttttatgtt aagcgactta
     1921 cccttgggat cgcgggcgat caccgtaaaa ttcttgagtg gattttcgtg tagataaatc
     1981 gtaatgggat aagcgccttc attcactaac gaaagcgtaa atataatcgg ttcatttttt
     2041 gcaaaacgca gtttttcagc cgacgcatag agtctcagtc cccgtgcact aaaattctcg
     2101 gttgcatttc cgtggtgatc caccttttga tggcgtggcg aaagcgccat aaccgacgag
     2161 agcgcaatag aaaccgcgta tagggaaatt ccccgtatca tacttttata tcgaccgaaa
     2221 tatgtctctt cattgagttc tttttcactg gtcagccgat ttacactgtg tctttaggtc
     2281 tatgctcaaa gaacgtctct ttacctttta tgaaaaaatg ctactcatcc gtcgctttga
     2341 agaggcggcg gcaaaagcgt acgcgacggg taaaattcgc ggattttgcc atttatatat
     2401 cggtgaagag ccggtggcag taggagcaat tcataatctt gaaccgcatg attatgtcat
     2461 cgccacttac cgcgaacacg gccatgcgct cgcccgaggc atgtcgcccc gcatggcgat
     2521 ggccgaactt ttcggcaaag cgacgggatg ctccaaaggt ttgggtggtt caatgcacct
     2581 tttctcgcgt gaactcaatt ttatgggagg ccatggcatt gtgggcggcc atgtcgccgt
     2641 cgccaccggc catgcgtttg cgtccaagta tcgcagcgac cacggcgtga cactttgctt
     2701 tgtaggcgaa ggtggaacaa acatcggcgc tttttacgaa ggcttgactt acgcttcgct
     2761 ctggaatcta ccggtggttt tcattatcga aaataattta tacgcgatgg gtacgccact
     2821 ctaccgttcc caaccgacac acgacttaac cgcgaaagcc aaagcgttca atatgccttc
     2881 cgagcgtatc gatgcattga gcgtagagaa agtgtaccaa acggtcaaaa aagcagtcac
     2941 ccgtgcacgc gccggattag gccccactct tttggaaatg atcacctacc gcttccgtgg
     3001 gcattcgatg tccgatccgg caaaataccg ccctcacggg gaactcgaaa aaatgaaaga
     3061 aaaagatccc atggacacca cgcgtgcaaa actcaaaaga cacggcttca ccgacgcccg
     3121 gttagaagaa ctcgacgcca aaattgaagc ggaagtcgaa gacgccgttc agtttgccga
     3181 agcgtcacca gaactcagct atcaagaaat gaaagaactg gtttttacaa ctacatagaa
     3241 gatcacacgt atatttacgt acgtagttca gttctatttt ttttgatatt atttgacaat
     3301 tatggtctga ttcataatgt tactatgtag tctggtacag actatgcgga gtagtgcacg
     3361 ctttcaatta agagtaagtt cgatggcctc atctcgcggg cctgattcaa tatttcttat
     3421 tcgatattga tttggggcac taccctcaag gtggggcttt cgattggtat ataaaatata
     3481 cacacaataa cgcttatgga gaaaaaaatg ggaaaaattc aacgaaccta cgtcggcatt
     3541 tcaggcaccg gcaagagtga attttttaac ggcatcatca accagttcgc agggacagag
     3601 gctgcaaacg aaattgcaat gccagatggc attgtgcgta acatccgctt ttataaatac
     3661 gatacgacga cttgttcttt tccaatcact attgtgcttc ggaaaaaccg cgtagacacc
     3721 catgtaaaaa ttgttatccc agcaggtgaa atgatacgcc ccacctcaat cgctgaaacg
     3781 gtgccgtaca atgcgggcga tgtgttggta tggaaagcgt cgattgcaac ctcttgttct
     3841 tgcccacaca gggtgggcgt ttgggccgtt tacgattatt agctcaagac tccctatcgg
     3901 cgcgaccgat accgcatgcg agtaatgctt ttcaatactc tcgtttaagc taatgagcct
     3961 gtgcgtcaac tcgcggagac aattttttta tagaacctat gcgacgtaat cttctaccgg
     4021 caaacacgaa cacaccaaat tacggtcgcc gtagacgttg tcgacacgcc ccacactggg
     4081 ccagaatttt tgtgccgcaa cgtacggtaa cgggtatgct gcttcgtggc gcgagtaact
     4141 atgcgtccac gcatccgcgc tcaccatttt tgcagtatgc ggcgcgccct tgagggggtt
     4201 atccgccttg tcgcttttgc catctgcaat acggcggatt tcctcgcgga tagcaatcat
     4261 tgcatcgcag aaccggtcga gttcggcctt cggttcgctt tcggtcggtt caaccataag
     4321 ggtgcccggc acgggaaagc tgaccgtcgg cgcatgaaaa ccataatcca tgagacgctt
     4381 tgcgatatct tctacctcga cgccggttgt ctgcttgaag acacgggtat cgagaatgag
     4441 ttcgtgtgca cagcgtccgc ggctacccga atacaatacc ggatagtgtt tctcgaggcg
     4501 cgctttgata tagtttgcat tcaaaattgc atagcgcgtt gcgttcgtta agcccgcgag
     4561 gccca
//
LOCUS       DAOMPD010000191         4528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_7991_length_4528_cov_2.689023, whole genome shotgun sequence.
ACCESSION   DAOMPD010000191 DAOMPD010000000
VERSION     DAOMPD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7991_length_4528_cov_2.689023"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1465
                     /locus_tag="PLY93_07690"
     CDS             116..1465
                     /locus_tag="PLY93_07690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HRP69396.1"
                     /translation="MKKKSSLWVFIFALVAVNGYLLYYHIYRAYRFDKLRERILSSAL
                     SSFDETALAGQALHIAIPGTTLNEQNKRILARVKPGGIIFFGFNLENAEQTRRFTADL
                     QNYVESLGLPPLLISTDQEGGYVKRVTDGVLQSPPPRDLGSVGDSGLCFSTGYYVSKG
                     LAKQGINVFFAPIVDINNNPENPVIGLRSFGDTVDAVLRCALPFEQGARAAFREGGAL
                     PVIKHFPGHGDTRIDSHWALPVIDKSLAALQAFELVPFREAVERGTRAVMTAHILYPQ
                     VDRDAPATLSQTWLTNILRGTLQFKGLIFTDAMEMSAVSKHYGKLNPPVAALKAGADV
                     LLYTSWQDEMLDAHQRIINEFSHPTQQNLQNPSALQRAVMNQLKAKLLYLKLEKYISP
                     EDAAWYRAYQNEIEKDAPKLQVHYKEEALKEKLKTIRWSKPRARNQPQWLAGQKNAR"
     gene            complement(1467..3320)
                     /locus_tag="PLY93_07695"
     CDS             complement(1467..3320)
                     /locus_tag="PLY93_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRP69397.1"
                     /translation="MRLLFLLFIFLFGVHHLFIFIFTHSAPLTASDLLSLVLADISAA
                     LFLSLLFQYLGKTRAYFFRILLYTFSFLFVLFLLMTFGFARLYERVFTWSFVRLDSLS
                     IWKENFISALYELNITDAFLFVTLLILLSVAIFLARKARPLRLRSIFFTGITILLVGF
                     LSYSFLPQQVNTLSNPVIAAFQKKKVRSEDNARVTADDTIPELDSLTGGFVKAPEVTR
                     KAGKNVILYFLESTPYSVIGQKIQNKEITPSLNAFKKESLFFERHYANFPLSINAFYS
                     SLCGAYALPDGAWVSLSLPDFPVPCLSQILKKHGYRILALHAGYLTYAKQKRFMLKRG
                     FDFMADAETIKKPPYEKGMGPWGAADERAMIQPLVNFINANAEKPFMAVLFAFAPHHP
                     YSIPDDFPEFITERGALKKQHRDYFNSLHFADMAFGKIRDALQKNGALENTVILVFGD
                     HGEAFYEHRGNYNHPFFIYEENVHVPFFLWYKGITPATLERVTSHVDILPTVLDILNL
                     SKEITPYHVGRSMLKGGAQTMAHLQAYWNEELSGIVDNHYKFIRKETGETELYNLLND
                     KEEKKNIANEIPELTAAYNQQTLKAFAQKKAYYKKYAQYDLVRFKPSSQDK"
     gene            3412..4116
                     /locus_tag="PLY93_07700"
     CDS             3412..4116
                     /locus_tag="PLY93_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69398.1"
                     /translation="MRYQWSNVKHPQTHDHRWEYPEVFTTSTSHYDHIKVEANRSTGQ
                     VRLEVKTLRGEHYESILKHGVILREKDIKTGALTDLTEILSIYRKDFSSLPDTPILEL
                     IGGNYGIMTEFLGTAERIVIEENSLKKKIVHFFKNIFPDLRKSFRNLFSVFKYYSNSM
                     RWADLFDMALIGSAAYAAFSYNFDFLNTGFTLALSAMATGLIDWLIRRREPWLMKIYA
                     SLAVASVAIYRGYFFQ"
     gene            4156..>4528
                     /locus_tag="PLY93_07705"
     CDS             4156..>4528
                     /locus_tag="PLY93_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRP69399.1"
                     /translation="MKDYIRNQIKASIDTKTAFLADEKNIELLDRVSQELIAAYRAGK
                     KTLTAGNGGSAADAQHIAAEFVSRFYFDRPALPSIALTTDTSALTAIGNDYGYEFLFS
                     RQLEANGQKGDFYIAISTSGNS"
BASE COUNT         1259 a         1051 c         1060 g         1158 t
ORIGIN      
        1 aacgcactca acttgacccc aaaagtgtca gttatctctg tgggttgcat ataaagaact
       61 atctttacag ttggcacaca gactcatggg caatttctgc caccattaaa gttttatgaa
      121 aaaaaaatcg tcgctttggg tgttcatttt tgccctcgtc gccgtcaacg gctatcttct
      181 ttattaccat atctaccgcg cgtatcgatt cgataaactt agagaaagaa ttttatccag
      241 cgctctttcc tcattcgatg aaactgcact cgccgggcaa gcgttgcata tcgccattcc
      301 cggcaccaca ctcaacgaac aaaataaacg cattttagcg cgtgtcaaac ctggcggtat
      361 tatttttttt ggttttaatt tagaaaacgc tgagcaaacc cgccgcttca ccgccgactt
      421 gcaaaattat gtcgaatcgc tgggactgcc gcctcttctt atttcaaccg accaagaagg
      481 cggttacgtc aagcgtgtca ccgatggagt gttgcaatca ccccccccac gcgatttagg
      541 gagtgtgggc gacagcggtt tatgtttttc caccggttat tatgtatcca agggactggc
      601 gaagcaagga atcaatgttt tttttgcgcc catcgtggac atcaacaaca acccagaaaa
      661 tccggtgatt ggtttgcgct ctttcggaga tacggtggac gctgtgttgc gttgtgcgct
      721 cccgtttgag caaggcgcac gtgcggcatt ccgcgaaggg ggagctctac cagttatcaa
      781 acattttcct ggccacggtg atacacgcat cgacagccac tgggcactgc cggtcatcga
      841 caaaagttta gctgcgctcc aagcgtttga gcttgtgccg tttcgcgaag cggtggagcg
      901 gggcacacgg gcggtgatga cggctcatat tctttatccg caagtcgaca gagatgcacc
      961 cgcgacgctt tcgcaaactt ggctcacgaa catcttacgc ggcacgcttc aattcaaagg
     1021 gttgattttc accgacgcga tggagatgag cgctgtttca aagcattatg gcaagctcaa
     1081 cccgccggtt gctgcattaa aagcaggtgc ggatgtactc ctctacactt cgtggcaaga
     1141 cgagatgctc gacgcacacc aaaggattat caacgaattt tcacacccaa cgcagcaaaa
     1201 tttacaaaat ccttctgcgc tccaacgcgc ggtgatgaat caactgaaag ccaaacttct
     1261 ttatttgaaa ttggagaagt atatttctcc ggaagatgca gcatggtaca gagcatatca
     1321 aaacgaaatc gaaaaggacg cacccaagct acaagttcat tataaagaag aagcgctcaa
     1381 agagaaatta aaaacgatac gttggtcaaa accgcgcgcc cgcaatcaac cgcagtggct
     1441 tgccggccaa aagaacgcgc gctgagctat ttatcttgac tcgacggctt aaagcgcact
     1501 aaatcatact gcgcatattt tttatagtac gcttttttct gcgcaaatgc cttcaacgtt
     1561 tgctggttgt acgcggcggt aagttcgggt atttcgttcg caatattttt tttctcctct
     1621 ttatcattga gtaaattgta aagctctgtt tcgcccgttt ctttgcggat aaatttgtag
     1681 tggttatcca caattcccga aagctcttca ttccagtacg cctgtagatg cgccatcgtt
     1741 tgtgctcccc ctttgagcat cgaacggccg acgtggtaag gagtaatttc tttagagaga
     1801 tttaatatat cgagcaccgt gggcaaaatg tcgacgtgac tggtgacgcg ctcaagagtg
     1861 gcaggagtaa tccctttgta ccagagaaaa aacgggacat gcacattttc ttcataaata
     1921 aaaaaaggat ggttgtagtt tccacggtgt tcgtagaacg cttcgccgtg gtcgccaaaa
     1981 acaaggatga cggtattttc gagcgctcca tttttttgta gcgcgtcgcg gattttccca
     2041 aaggccatgt cggcaaaatg gagcgagttg aaataatcgc ggtgttgttt cttcagcgcg
     2101 ccgcgttctg taataaactc ggggaaatca tcggggatgc tgtaggggtg gtgcggcgcg
     2161 aaggcaaaaa ggactgccat aaaaggtttc tccgcattcg cgttgatgaa atttacaagt
     2221 ggttgaatca ttgcacgctc atcggctgcc ccccaagggc ccatgccttt ctcgtacggc
     2281 ggctttttga tagtctctgc atccgccata aaatcaaacc ctcgttttag catgaaacgc
     2341 ttttgcttcg cgtacgttaa ataccccgcg tggagcgcga ggatacggta gccgtgtttt
     2401 ttcaaaattt gagaaaggca gggcaccgga aaatcgggca gcgagagcga tacccacgcg
     2461 ccatcgggga gcgcgtatgc gccgcaaaga gaactgtaga acgcgttgat gctgagcgga
     2521 aagttggcgt aatggcgttc aaaaaaaagc gactcttttt tgaacgcgtt gaggctgggg
     2581 gtgatttctt tattttggat tttttgtcca atgacagaat acggtgttga ttccaaaaag
     2641 tagagaatca catttttgcc cgctttgcgc gtcacttctg gcgctttcac aaagccaccc
     2701 gttaagctat cgagctcggg gatggtgtcg tcggcggtaa cgcgtgcatt atcttcgctc
     2761 cttactttct ttttctgaaa cgccgcaata acagggtttg agagagtatt gacctgctgc
     2821 ggtaaaaaag aatacgataa aaatcccact aaaagaatag ttatacccgt aaaaaaaata
     2881 gagcgtaaac ggagcgggcg agctttgcgc gctaaaaaaa tggcaacgct cagtaggatt
     2941 aaaagcgtca cgaataaaaa cgcatcggtt atattgagtt catagagcgc cgaaataaaa
     3001 ttttctttcc aaatcgaaag cgagtcgagt cgcacaaatg accacgtaaa gacacgctcg
     3061 taaagtcgtg caaaaccaaa cgtcatgagt aaaaaaagaa caaagaggaa tgaaaatgta
     3121 taaagaagta ttctaaaaaa atatgcacgc gttttgccaa gatattgaaa aaggagagat
     3181 aaaaaaagcg cggcgctgat gtcagcgaga acaagcgata gtaggtcgct tgctgtcagc
     3241 ggggcagagt gagtaaaaat aaaaataaat agatggtgga cgccaaacaa aaaaataaag
     3301 agaagaaaaa gcaaacgcat gggtgttgaa agcggtaact cacacgccgc gtcatccttt
     3361 tcaaaagtca aaaataagaa gaaaattccc gcgatccacc gatattaaaa tatgcgctac
     3421 caatggtcaa acgtcaaaca cccacaaacg cacgaccacc gttgggaata ccccgaggtg
     3481 ttcacaacga gtacgtcaca ctacgaccac attaaagtcg aggcgaaccg ctcgacaggt
     3541 caggtacgtt tagaggtgaa aacgctgcgc ggcgaacact acgaaagcat tcttaagcac
     3601 ggcgtcattt tacgcgaaaa agatataaaa accggtgcgt tgaccgacct cactgaaatt
     3661 ctaagcattt atcgtaaaga ttttagctcg ctgcccgaca cgccgattct agaactcatc
     3721 ggcggtaatt atggaattat gaccgagttc ttaggcacgg cggagcgcat tgtcatcgaa
     3781 gaaaattctt tgaaaaaaaa gattgtacac tttttcaaaa acatttttcc tgatttacgt
     3841 aaatccttca ggaatttatt ttctgtattc aagtactatt ctaattcgat gcgttgggct
     3901 gacctttttg acatggcgct catcggctcc gctgcgtatg cggcgttttc gtacaatttt
     3961 gattttttga atacgggttt tactcttgca ttgagcgcca tggccaccgg tcttatcgac
     4021 tggctcatcc gccgccgcga accgtggctc atgaaaattt acgcttcgct cgctgtcgcg
     4081 tcggtcgcca tttatcgcgg ttattttttt caataattat ggcgacacgc cgttaagtat
     4141 agctatatta gccccatgaa agattatatt cgtaaccaaa ttaaagcgtc gatagacacc
     4201 aagaccgctt ttttggccga tgaaaaaaat atcgaacttt tagaccgcgt ttctcaagag
     4261 ctcatcgccg cgtaccgtgc gggtaaaaaa actctcaccg ctggcaacgg gggaagcgct
     4321 gccgatgcac aacacatcgc tgccgagttt gtcagtcgtt tttatttcga tcgccctgcg
     4381 ttgccttcga ttgcattgac taccgacaca tcggcgttaa ccgccatcgg taacgattat
     4441 gggtacgaat tcttattttc acgccaactg gaagccaatg gccaaaaagg agatttttat
     4501 atcgccattt cgacatcggg gaattcaa
//
LOCUS       DAOMPD010000192         4506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8045_length_4506_cov_2.548191, whole genome shotgun sequence.
ACCESSION   DAOMPD010000192 DAOMPD010000000
VERSION     DAOMPD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_4506_cov_2.548191"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1021)
                     /locus_tag="PLY93_07710"
     CDS             complement(191..1021)
                     /locus_tag="PLY93_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69400.1"
                     /translation="MQQTTQNSKSGAHRSAKKTISIGDYLSMGNRSASAKSESLLKKK
                     NPPQKELSLNEVEELSRIKAEAKRAYRRKDFVQALARFDDALAILPGDLEFSYYQALC
                     LFQLHQWDKAEELFERIAELDELRLLPDVRKMLALTLLKRRKFIEAENVLIEATRDRK
                     NDAQLMNMLGYALERQNKLIEAERVLGNLMDKDPENVNGMNSLAMVYCRLEKNYTEAL
                     QLVKRALAKEPRNAYYLDTLGMLYATRGNRTAAKKTLQKALEYAPGHPEIVSHLSRVS
                     "
     gene            complement(981..1709)
                     /gene="surE"
                     /locus_tag="PLY93_07715"
     CDS             complement(981..1709)
                     /gene="surE"
                     /locus_tag="PLY93_07715"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRP69401.1"
                     /translation="MRILISNDDGLQFPGLLRLREKLEKFGETWVFAPAEEKSATSNA
                     LTIYKDIRVVKQAERTYQVDAFPADCVNVALHGGFAPNFDFCVSGINKGVNMGQDVWY
                     SGTVGAARHAYIHNVPSFSVSCGYLDATGDFNKVADIFCEIFARFASNADQNFLLNVN
                     IPMTAVDASDIKWAKLGRRIYRDNYKRVVQDDGGMLLNLGGSVLSHGGEADTDFDWYD
                     KGYTVITPLTIDATNYTKLKERCA"
     gene            complement(1718..3256)
                     /locus_tag="PLY93_07720"
     CDS             complement(1718..3256)
                     /locus_tag="PLY93_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69402.1"
                     /translation="MGKFTQALQFYARLIKKYPKHTKVFDAEMGIGWTYFELKQYARA
                     GDSFRKILRKYKKSDEQAKAYLALGACLYNLRDLQGALKSYKTILEKFLTQHDEATEA
                     RYQMAWLHFRQNKFDEAILEFQKYIALGENEPRHADALYFSGLSYFQTGKYAEAINHF
                     ERAAHTPNAPAWLKEKALADLARSQNSAGQIADAQKTYKALIEAYPQSSVYDEALYQL
                     VLLAFKTNDEASAIEWKGQLKAHNSKSPWYAEALRETADFYRKEKRFTLAEHALVELE
                     EAQKKPEEKLTTLVLRASLHFEEGRDEKAKALTLKILQNEEITDATANSAAALLFRIY
                     EKQGAYVDGEKEAVRLCTRFAENSLLKEEMLLHQAHFLTLQKKFSASREALTPLATSR
                     HTGARARFAIAETYRAENDTTHALDFYRQITQKQDEGLYLKARFYIGQILFDRKDYEA
                     AAREFSRVAYADSRDDATYEKALYYAARAFREIQKEKEFETFREKLKEAFPQSTYIKE
                     LETP"
     gene            complement(3257..>4506)
                     /locus_tag="PLY93_07725"
     CDS             complement(3257..>4506)
                     /locus_tag="PLY93_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69403.1"
                     /translation="RSAMAAFKERNFYSARLLLQEIALKDARGEYGDDAQYYTALSYY
                     YEGEYKAAQFEFKSLMRDYPDSPFVVRAFFWTGEAWFYRKAYREALESHAAFVRKYRE
                     NPLAASALYTMGFIYNEQNRYDEAVDVLERALKDYPKTNVVPALTLQLGIALFNAKEY
                     AKARRHFETLLVNYTQAENLDEARFWLGKSYYAENKFDEALREFDAVVKDYPKSKSAS
                     EALYLAALSKYKQRALDEALLYLARVTHEYPQSAMYPFARLREAQLFYEKKDWDAALI
                     PILDLVDNHREHETFFAALQLMAEIRQKQGKTAEALKALEALRTAKNLQGKNRRDMLR
                     YYSDLLYQEKQYATAAAVLDELTEEYSGDNDAATYFLLRARAEFRDNRSTDAQKTLAQ
                     LEKKYNDAKVHAEALFLKAEIAY"
BASE COUNT         1019 a         1074 c         1098 g         1315 t
ORIGIN      
        1 ccacacccgg ttattggggt ggcatcgaat ggctttcagg ttctgtaagc tcggcgattt
       61 cgggtggtta tggcggtggc ctttgtattc ggcgtcttct tcgggcgatt gtgtaggtgc
      121 ccttaagggg cctccgtttt tctcctaaaa tttcaatgca gccagcgaga aaaggccgtt
      181 atctctaaaa ttaagacacg cggcttaaat gcgaaacgat ttcagggtgt cctggggcat
      241 attcgagggc tttctgcaat gtttttttgg ccgccgtgcg attaccgcgt gttgcgtaga
      301 gcatccccag cgtgtcgaga taatacgcgt tgcgtggttc tttcgccagc gcccgcttga
      361 cgagttgcaa cgcttcggta taattttttt caaggcggca atagaccatt gcaagagagt
      421 tcatgccgtt cacattttcc ggatctttat ccatgaggtt gcctaaaacg cgttcggctt
      481 caattagttt attttggcgt tcaagcgcat agccgagcat attcatgagt tgggcgtcgt
      541 tcttgcggtc acgcgttgcc tcaatgagaa cattttctgc ttcgatgaat ttgcgccgct
      601 tgagtagagt cagcgcgagc attttacgca catcgggtaa aagtcgcaat tcgtcgagct
      661 ctgcaattct ctcaaaaagt tcttcggctt tatcccactg gtgtagttga aaaaggcaca
      721 gcgcttggta atacgaaaac tccaaatctc ccggtaaaat ggcgagtgca tcgtcgaagc
      781 gcgcgagcgc ttgcacaaaa tctttgcgcc ggtatgcgcg cttcgcctcc gctttaattc
      841 gtgaaagctc ttctacttca ttgagtgaaa gttctttttg agggggattc ttttttttaa
      901 gcaacgattc gcttttagcg ctggcgctcc gattgcccat cgatagataa tcgccgattg
      961 aaatcgtttt ttttgcgctt ctatgcgcac cgctctttga gttttgtgta gtttgttgca
     1021 tcaattgtca gcggagtgat gacggtatag cctttatcat accagtcgaa atctgtatcc
     1081 gcttcacccc catggcttaa taccgagccg cccaagttga gtaacatacc gccatcgtct
     1141 tgaacaaccc tcttatagtt atctcggtag attcgccttc ccaatttagc ccattttatg
     1201 tcgctggcat cgaccgccgt catagggata ttcacattga gcaaaaaatt ctgatcggca
     1261 ttcgaggcaa agcgggcaaa aatttcacaa aaaatatcgg caactttgtt gaaatcaccc
     1321 gttgcgtcca gataaccgca cgacaccgaa aatgaaggca cgttgtgaat gtacgcgtgc
     1381 cgcgcagcgc cgacggtacc ggaataccac acatcctgtc ccatgttgac gcctttattg
     1441 atacccgaaa cacagaaatc aaaattgggt gcaaagccgc cgtgcagcgc gacgttcacg
     1501 caatcggcgg ggaaagcgtc gacttggtat gtcctctcgg cttgtttcac tacacgaatg
     1561 tctttgtaaa tggtgagcgc gttgctggtg gcgctttttt cctcggcagg agcgaacacc
     1621 cacgtttcgc caaatttttc cagtttttca cgtagtcgta aaagtccggg gaattgcaaa
     1681 ccgtcgtcgt tagagattaa aatacgcatt gttgtcctta tggagtttcc aattctttaa
     1741 tgtaggtact ttggggaaac gcctctttaa gtttttctcg aaatgtttca aattcttttt
     1801 ctttttgaat ttcacgaaac gcgcgcgccg cgtagtacaa cgctttttcg tacgttgcat
     1861 cgtcacggct gtcggcgtaa gcgacacggc taaactcgcg cgctgctgct tcgtaatctt
     1921 tgcgatcgaa taaaatttgg ccgatataga aacgcgcttt caaatacaac ccttcgtcct
     1981 gtttttgagt aatttgtcga taaaaatcga gcgcgtgggt ggtgtcgttt tctgcgcggt
     2041 acgtttcggc gatagcaaaa cgggcacgcg caccggtgtg gcgacttgtt gcaagcgggg
     2101 tgagcgcttc tcggcttgcg ctgaattttt tttgtaaagt taagaaatgc gcttggtgca
     2161 gcagcatttc ttctttaagt aaagaatttt ccgcgaaacg tgtgcagagc cgcaccgcct
     2221 ctttttcacc gtcgacgtac gcgccttgct tttcgtaaat gcgaaaaagt aacgctgcgg
     2281 cgctatttgc ggtagcgtcg gtgatttctt cgttttgtaa tatcttgagc gtcaacgctt
     2341 ttgctttttc atcgcggcct tcttcaaaat ggagagacgc gcgcaacaca agcgtcgtta
     2401 gtttttcttc gggctttttc tgcgcttctt ctaattcgac aagtgcatgt tcagcgagtg
     2461 taaatcgctt ttctttgcga tagaaatccg ccgtttcgcg cagcgcctcg gcataccacg
     2521 gcgacttact gttgtgcgct ttgagttggc ctttccattc aatagcagag gcctcgtcgt
     2581 ttgttttaaa cgccaaaagg acgagttgat atagggcttc gtcgtaaacg ctactttgcg
     2641 gatacgcctc aatgagcgct ttatacgttt tttgagcgtc ggcaatttgc cccgcgctat
     2701 tttgcgaacg cgctaaatcg gcgagggctt tttctttaag ccacgccggc gcattgggtg
     2761 tgtgcgcggc acgctcaaag tgatttatcg cttcggcata tttgccggtt tgaaaataag
     2821 aaaggccgct gaaataaagc gcgtcggcat ggcggggctc gttttcaccc agcgctatat
     2881 acttttggaa ttcaagaatt gcttcgtcga atttattttg gcggaaatga agccaagcca
     2941 tttggtagcg cgcttcggta gcttcatcgt gctgtgtcaa aaatttttcc agaatcgttt
     3001 tgtaactttt gagcgcccct tgcaaatcgc gtaagttata aagacacgcc cccaacgcca
     3061 agtatgcttt ggcctgctcg tcgctttttt tatacttgcg caaaattttg cggaacgaat
     3121 cgccggcgcg tgcgtactgt tttaattcaa aataggtcca accgataccc atttcggcgt
     3181 caaaaacttt tgtgtgcttt gggtatttct taatgagtcg tgcataaaac tgcaacgctt
     3241 gggtaaattt acccaactag tatgcaatct cagcttttaa gaaaagcgct tcggcgtgaa
     3301 ctttggcgtc gttatatttt ttttcgagtt gcgccaatgt tttttgtgcg tcggtagaac
     3361 gattgtcgcg gaactcggct cgtgcgcgta ataaaaaata agtggcagca tcgttgtcgc
     3421 cgctatattc ttcggtgagc tcgtcgagca ccgccgcagc ggtggcgtat tgtttttctt
     3481 ggtagagtaa atcgctgtaa taacgcagca tgtcacgacg atttttaccc tgaagatttt
     3541 tcgccgtgcg caacgcttcg agcgctttta acgcctcggc agttttgccc tgcttctgcc
     3601 gaatttctgc cataagttgt aatgcggcaa aaaaagtttc gtgctcgcgg tggttatcga
     3661 cgagatcaag aatagggatt aaagcggcgt cccaatcttt cttctcataa aacaactgcg
     3721 cttcgcgtaa acgcgcaaaa ggatacatag cgctttgtgg gtattcgtgg gtaacgcgcg
     3781 caagatagag aagcgcttcg tcgagcgcac gttgtttata cttggaaagc gctgccaagt
     3841 acaacgcttc gctggcgctt ttcgatttag gatagtcttt aacgacggcg tcaaattcac
     3901 gcagcgcttc gtcgaattta ttttctgcat aataactttt acccaaccaa aaccgcgctt
     3961 cgtctaaatt ttcggcttgg gtataattga ctaaaagcgt ttcaaaatgg cggcgtgctt
     4021 ttgcatattc tttggcgtta aaaagagcaa tgcctaactg caacgtcagc gcaggcacaa
     4081 cgtttgtttt gggataatct ttgagcgcgc gctccaatac atccacggct tcgtcgtaac
     4141 ggttttgttc gttgtaaata aaacccatcg tgtaaagcgc cgacgccgct aacggattct
     4201 ccctatattt acgcacgaat gctgcgtggc tttccaacgc ttcgcggtat gcttttcggt
     4261 agaaccacgc ctcgcccgtc caaaaaaagg cgcgcacaac aaaaggcgag tcagggtagt
     4321 cacgcatcaa tgatttgaat tcaaactgcg ccgctttgta ttcaccctcg tagtaatacg
     4381 aaagcgcggt ataatactgc gcatcgtcgc cgtattcgcc gcgcgcgtct ttgagggcaa
     4441 tttcttgcag tagaaggcgc gccgaataaa aattacgctc tttgaacgcg gccatcgcag
     4501 agcgaa
//
LOCUS       DAOMPD010000193         4505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8050_length_4505_cov_2.059326, whole genome shotgun sequence.
ACCESSION   DAOMPD010000193 DAOMPD010000000
VERSION     DAOMPD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8050_length_4505_cov_2.059326"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLY93_07730"
     CDS             complement(<1..309)
                     /locus_tag="PLY93_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP69404.1"
                     /translation="MKINSILEAIGNTPHLRAKSLFPDAEVWVKLERQNPGGSLKDRI
                     AREMVEDAERRGVLKPDTTIIEPTSGNTGVGLALVGAAKKYKVILVMPESMSVERRAI
                     M"
     gene            532..981
                     /gene="fliS"
                     /locus_tag="PLY93_07735"
     CDS             532..981
                     /gene="fliS"
                     /locus_tag="PLY93_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRP69405.1"
                     /translation="MSHPMQRDLHLKEYQANSVGTADQKQLIIMLYEGAERFIKEAEG
                     FMVSFKTYDKANVAILRAQDIFTELMVSLNLEHGGEIAQNLFNLYAYAKAQLLDANVE
                     KKVEKLFPVKKILKDLLEAWRQVDTQPTTKNPQTKPADYKGGFTAEG"
     gene            1083..1223
                     /locus_tag="PLY93_07740"
     CDS             1083..1223
                     /locus_tag="PLY93_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69406.1"
                     /translation="MVERRSKTLQEIENLSSPSSLADSVRLGLARYLNREAIKMVHQI
                     IT"
     gene            1532..>2553
                     /locus_tag="PLY93_07745"
     CDS             1532..>2553
                     /locus_tag="PLY93_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69407.1"
                     /translation="MPPRLPPSQPLRTKDEEASPKDSRWSRIKLAFSRFFLRRIGFAH
                     DATDMLRSAMKLGNPTKAQWPSETIPMNARIFASGFWNYSFFQFNRKFLPPFWAEEQY
                     NPESKSFLPRSHNVLSLNQTQRNWVAIGFPGRSLEASLDMAGSLMVYPGSYTVEFATI
                     ENERIVRPQNDVQYVHLELKSSYELHIAWRDIRIIYTATAYGIHFKATGDSPLLLSIR
                     PFNFEGPALLYKLHYNERKLQLTGDAEFSFDAPPTRVLLSNRRKGDALRRIAAQMRSP
                     QEGDTEIFDVKDTIGLATGAFYFARASLASGKIFDRQDDALPASITQKSAEGITSHFF
                     SNTLTA"
     assembly_gap    2554..2595
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2596..3880
                     /locus_tag="PLY93_07750"
     CDS             <2596..3880
                     /locus_tag="PLY93_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69408.1"
                     /translation="LEHAKHHLVALWDFDSIKPGSYTYHHFWIRDAVIMMYALLLVGA
                     KHAVKPILERFTGLVKRSGLFRSQSGEWDANGQALWILAQYARLTQDTSVIDSMKKHI
                     TRMLSWYEKTLAHHGGVLPPGFSAEHLGPADWYLWDNFWALGGLKEIAPYLNDAAMVQ
                     RATTIYKTLSMRLGEYLNSYPYLPAALGRSKDAGMIGSIIVGYPLQVTDFLDARYLDS
                     LDIIHKNYFFQGCFFQENIHSGLNPYLTLQMAESYLFVGKTGAARKILRNIKRRAQKA
                     YSYPEAIHVRTGGGCMGDGFHGWASAEAVIMIRNLVVREVTLKTKTSALVWLAGYREK
                     WLYEKSYVENIYTQHSHATLTLSDCHLTLENISMKETHILSLPQGFVPVDTTTNKALP
                     YFAHENFIPVADTERNYFLLDKDDGKKTTLHLAKP"
     gene            3898..>4256
                     /locus_tag="PLY93_07755"
     CDS             3898..>4256
                     /locus_tag="PLY93_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69409.1"
                     /translation="MILYTGTLAPHHTGLNLAWEDFFLRSQKENGRMFLVFYENFSSM
                     VLGKSLEIEKEIFTDKRHPPVYRRISGGGSVFHSKGNLNYSLFLSLADFPELNNVTVS
                     YQKILTALAVSLGRHVA"
     assembly_gap    4257..4278
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4279..>4505
                     /locus_tag="PLY93_07760"
     CDS             <4279..>4505
                     /locus_tag="PLY93_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRP69410.1"
                     /translation="ALFSRGAWRKFSGNAQCRKQGWIMHHGTLLYSKKIIPRISYYLK
                     PPPKEPEYRLGRNHRTFMTTVLPCYSRATLM"
BASE COUNT         1190 a         1167 c          968 g         1116 t
ORIGIN      
        1 catgatagca cgacgctcca ccgacatact ttctggcatc acgagaatca ctttgtactt
       61 tttggccgcg cccacaagcg ccaagccaac accggtgttt cccgaggtgg gttcaataat
      121 agttgtgtct ggttttaata cgccacgacg ctcagcgtct tcgaccattt cacgcgcaat
      181 acgatctttg aggctaccgc ctgggttttg ccgttcaagc ttcacccaga cttctgcgtc
      241 gggaaataag cttttcgcac gcaggtgcgg tgtgttgcca atggcctcga gaatactatt
      301 tattttcatt gtgtgctcct aatctatttt cgctgcaaca tcaaaacaac tacttgtctg
      361 ttatattgaa cagcgtcacg agcgacgggt tcctcaatat gactccagtc gtcaactaaa
      421 acataccttt gttcgttaga ttgaaagacg caccagctca caggatccac tctcccaaaa
      481 attttcttgc gaaaaagcta aaaatagccg acagtgtgat agcggcttaa aatgtcgcat
      541 ccgatgcaac gcgatctgca cctcaaagaa tatcaagcca attcggtagg taccgccgac
      601 caaaaacagc tcatcattat gctgtacgaa ggcgccgaac gctttattaa agaagccgaa
      661 gggtttatgg tctcgttcaa gacatacgac aaagcgaacg tcgctatttt gcgtgcacaa
      721 gatattttca ctgaactcat ggtttccctc aacctcgagc atggcggaga gattgcacaa
      781 aatcttttta atctatacgc gtacgccaaa gcgcagcttt tagacgcgaa tgttgaaaaa
      841 aaagtcgaga agctttttcc cgtaaaaaaa atcctcaaag acttgctcga agcttggcgg
      901 caagtcgaca cacaaccgac aacaaaaaat ccacagacga aacccgccga ctataaaggc
      961 ggatttaccg ccgaaggata actattctac cagaaaaatt actcctccaa tcgctgcgca
     1021 tttaccgcgt cgcacaaagc ccacgctttt ccctggaaaa aagaggggat accctaaaag
     1081 agttggtaga gcgccgctct aaaacactac aagaaataga aaatttatct tccccctctt
     1141 ctcttgccga cagtgtacgc cttggattag cgcgctactt gaaccgtgaa gcgataaaaa
     1201 tggttcacca gattattact taagagccta tgcgccaact cgcgcagcgt gtataaagag
     1261 gcaatttttt tgcaaatttt tgtacgcggt tcgctcaaaa acgcgaaatg ttgcacaaat
     1321 tcgggcgtct tcgcccatgc aaagcaaatt tgtacaattt cacgtcggcc aaagagccgc
     1381 tggcctcacg gcggtgcacg cagctttggc cgaggaattt gcgcaaaata gagtacaaaa
     1441 aattgtctca gcgagttggc gcactggctc ttaaaatact aactccatcc aatttcatta
     1501 tacgcgctat atccaaaaag gagaattacc tgtgccgcca cgcctaccgc cctcgcaacc
     1561 tcttcgtact aaagacgaag aagcgtctcc taaagactcg cgttggtcgc ggataaaact
     1621 cgctttcagc cgtttttttt tacggcgcat cggatttgca cacgacgcca ccgatatgct
     1681 gcgctcggcg atgaaattgg gaaaccccac caaagcgcaa tggccgtcgg aaacaatccc
     1741 gatgaacgca cgcatctttg cgtcggggtt ttggaattat agtttttttc aattcaaccg
     1801 taaattctta cccccttttt gggcagaaga gcagtataat cccgaatcca aatcgtttct
     1861 gccgcgttcg cacaatgttt tatcgctcaa ccaaacgcaa cgtaactggg tcgcgattgg
     1921 ttttcccggg cgcagcttgg aagcatcgct cgacatggcg ggtagtctca tggtgtaccc
     1981 gggttcgtac accgtcgaat ttgcgacgat tgaaaacgaa cgtattgtgc gtccacaaaa
     2041 cgacgtacag tacgtgcatc tggaactaaa gtcatcctac gaactccaca ttgcgtggcg
     2101 tgacatccga attatttaca ccgcgacggc atacggtatc cactttaaag ccacgggaga
     2161 tagccctctt cttctttcga ttcggccgtt taattttgag ggccctgccc ttctttataa
     2221 attacactat aacgaaagaa aattacaatt aacgggcgat gccgaattta gcttcgatgc
     2281 acctcccacc cgtgtgctct tgtcgaaccg acgaaaaggc gatgcgctac ggcgtatcgc
     2341 tgcacaaatg cgcagcccgc aagagggtga cactgaaatt tttgacgtga aagacaccat
     2401 cggtttagca accggcgcat tttattttgc acgcgcttcg cttgcatcgg gtaaaatttt
     2461 tgaccgccaa gacgatgcac tccccgcaag catcactcaa aagagtgcag aggggatcac
     2521 atctcacttt tttagtaaca cactcaccgc acannnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnngttgg agcacgccaa acaccatctt gtcgctctgt gggattttga
     2641 ctccatcaag cccggttctt acacctacca ccacttttgg atccgcgacg cagttattat
     2701 gatgtatgcg cttttactcg tgggcgcaaa gcatgcggta aaacccattc tcgaacgttt
     2761 cacagggtta gtgaaacgct caggcctttt ccgctcgcaa agcggtgaat gggatgccaa
     2821 cggccaagcg ttgtggattc ttgcgcagta cgcacgcctc acccaagaca cctcagtcat
     2881 cgatagcatg aaaaaacata tcacacgcat gctgtcgtgg tatgaaaaaa cactcgcaca
     2941 ccatggcggc gtcttgcccc cgggtttctc cgccgaacac ctgggacccg ccgattggta
     3001 tttgtgggac aatttttggg cgttgggtgg cctcaaagaa atcgcccctt atttgaatga
     3061 cgctgcgatg gtccaacgcg ccaccacaat ctacaaaact ctctcgatgc gcttaggtga
     3121 atacctaaac tcgtacccat acttacccgc tgcgctcggg cgcagcaaag acgcagggat
     3181 gatcggttcg attatcgtgg gctatccgct ccaagtaact gattttctag acgcgcgcta
     3241 tttagatagt ctcgacatca ttcataaaaa ttattttttt cagggctgct tctttcaaga
     3301 aaatatccat tctggcctca acccttactt gacgctacaa atggccgaga gttatctttt
     3361 tgtcggcaaa actggcgcgg cgcgtaaaat tttacgcaac atcaaacgcc gtgcgcaaaa
     3421 agcgtattca taccccgaag cgatccacgt acgcaccggc ggtggttgta tgggcgacgg
     3481 tttccatggt tgggcgtcgg cagaggcagt catcatgatc cgtaatctcg ttgtgcgtga
     3541 agtgacgctc aaaacaaaaa catctgctct ggtgtggctt gcggggtatc gtgaaaagtg
     3601 gctatatgaa aaatcgtacg ttgaaaatat ttacacacaa cattcgcacg ccacgctcac
     3661 tttgtcggat tgccacttga cactcgaaaa tatttcgatg aaagaaacgc acatcctctc
     3721 gctaccgcaa ggttttgttc ctgtcgacac aacaaccaac aaagcactac cctactttgc
     3781 gcacgagaat tttatccccg tagccgacac cgaacgcaat tattttttac tcgataagga
     3841 tgacggtaaa aagaccacgt tgcatctcgc taagccctaa agcttgtgca aaccactatg
     3901 attctttaca caggcacact cgcaccgcac cacaccggac ttaatcttgc ttgggaagat
     3961 ttcttcttgc gttcgcaaaa agaaaacggt agaatgtttt tagtctttta cgaaaatttt
     4021 tcatcgatgg tgttgggaaa atccctcgaa atagaaaaag aaatttttac cgacaaaagg
     4081 catccccctg tgtaccgtcg catttcaggc gggggctccg ttttccacag caaaggaaat
     4141 ttgaattact cgcttttttt atcgctcgca gattttcccg aactcaacaa tgtcaccgta
     4201 tcgtatcaaa aaattttaac cgcgctcgcc gtatcgttgg ggcgtcatgt cgcaatnnnn
     4261 nnnnnnnnnn nnnnnnnncg cgctcttttc gcgcggtgcg tggcgtaaat tttcaggcaa
     4321 cgcgcagtgc cgcaagcagg ggtggattat gcaccacggt acattgcttt atagtaaaaa
     4381 aataatccca cgtattagct attatttaaa gccgccaccc aaagagcccg aataccgctt
     4441 ggggcgtaat caccgtacct ttatgacgac cgttttgccg tgctattcgc gcgccacgct
     4501 gatgc
//
LOCUS       DAOMPD010000194         4488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8092_length_4488_cov_2.953305, whole genome shotgun sequence.
ACCESSION   DAOMPD010000194 DAOMPD010000000
VERSION     DAOMPD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8092_length_4488_cov_2.953305"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1026)
                     /locus_tag="PLY93_07765"
     CDS             complement(67..1026)
                     /locus_tag="PLY93_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69411.1"
                     /translation="MKFLPHYFFLRYMLLSTAALIALQSLSAAPAAKPDFAKPKTFLN
                     LADRFADEQDYYRAITEYKKVIFLFPEYEKLDWVHFQIGKMYYEGGRFPQAKNELIPL
                     TEAKDEKLQFYALNYIALSYFENEEYSNAERLFLQLKEKDEGKIYWLDYTIYAGMATA
                     GNHNFAEAYTRIENAQNEWKKSSVKGDKFHAQYGNFFEKAIPLLDDARKLSEKSPFWA
                     TLFSILLPGGGHVYLGEWDTGIVSFTLVGGAAFLAYDGFMKDNMVQSIIFTTFATGAY
                     IGQAYSSYRTARKYNAELGNEEWRTLKREFHSLNVALQFQSQF"
     gene            complement(1044..1406)
                     /gene="yidD"
                     /locus_tag="PLY93_07770"
     CDS             complement(1044..1406)
                     /gene="yidD"
                     /locus_tag="PLY93_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRP69412.1"
                     /translation="MIYKTRYILFGVLSTMLCTLHAEEPWSISMRHAVTQDAILPQPH
                     QRISLMSDSNTLIRFYQKYISPLSGATCHYIPTCSQYTAEAVQKYGLFKGIVMGTDRL
                     IRCHPNQKEYPYDPPVTY"
     gene            1567..2208
                     /locus_tag="PLY93_07775"
     CDS             1567..2208
                     /locus_tag="PLY93_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69413.1"
                     /translation="MRRMLIIMAIAVFSMPYLYAVPFTWESKADEPGFVVDVPASWSK
                     ASRARDGVGNVHFEKKEKTGRVAIEVRAYSTEDTDIDQLVLQLRTRLAVKYDRLYLQK
                     RQEMTFRKGVEKQIWNARVGKIKYALTTAFVVADNRVLQITCIAPVSQAKRFTILFDN
                     ALLSLDFGDGSAAEPASAEAPAETPAATPVAPESAVNPPAAPSGKPQPPVIQF"
     gene            2230..3318
                     /locus_tag="PLY93_07780"
     CDS             2230..3318
                     /locus_tag="PLY93_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69414.1"
                     /translation="MKKLRHYFILLILIFPLMLTAEDTPSGADELEELKAMAKEMSDL
                     WGERVCASDVTHRYEETVVSIACNAEYQSAERDRMLDYLFKKGYFFRGESYNVKGTTG
                     HYWYKLGKKEGSRTRNLFIRLHITSQGALWGSVKPPTQIALYVDYLSSSDELTAWQTL
                     GVPVTFGLKVSEGAKELLQQLEEYKQEAWVSLDLRQGTFSEPDQTASIRDIIEQDLIA
                     PHIKNAIESTGEVMGFVMRDLNNITTTVASARAIFNAMKAEGKTMVLLPARHNAALST
                     TANVMGMNNRAVTYDMTMMCKKDPGKIWSFLKSKTNAGGIIVRFQAKDKRCAYRLSRT
                     MRRDGKLEYKKLSSIFGYNPARTEGRGD"
     gene            complement(3365..4156)
                     /locus_tag="PLY93_07785"
     CDS             complement(3365..4156)
                     /locus_tag="PLY93_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69415.1"
                     /translation="MRLFIFNLFLFSFFISPLFAEGAISFTLDGKTYSLADAKTAFIQ
                     KGDKSQLIIGVTDRTARVQFALTVESPMFTTPQQFTTSSNSISLTVLTTKGMYSVVPE
                     ITFARDDFMRYNRRKEVETGEMEDDPNDTSDNLPPECRTMHNTLQLQTAAHKASYERC
                     EKRHKTERKKRKKIRVEYVQEPPTWVNKTREERLRTGDGVMREEQYRDTFFQVNLTPT
                     LVDGKITQLTGSFGGTVRFAEGQFKGRTVTLSTGSFTVPVTYSKN"
BASE COUNT         1205 a          979 c         1123 g         1181 t
ORIGIN      
        1 gcaaaattgg ctgcatagct gtcaccgatg tctcggggtt ctacataaat taagaactta
       61 tacttcttaa aattgggatt gaaactggag cgcgacgttc aaactatgga actcacgctt
      121 gagtgtgcgc cactcctcgt tgcctagctc ggcgttgtat ttgcgcgcgg tgcggtaact
      181 cgagtaggct tggccgatgt aagcgccggt ggcgaacgtg gtgaagataa tcgattgtac
      241 catgttgtct ttcataaagc cgtcgtatgc caaaaacgcc gcgccgccca caagagtaaa
      301 gctgacaatt ccggtgtccc attcgcctaa ataaacgtgc ccgcctcccg gtaaaagtat
      361 cgaaaagaga gtcgcccaaa atgggctttt ttcagaaagc ttgcgggcat cgtctaaaag
      421 ggggattgct ttctcaaaaa aattaccgta ctgtgcgtgg aatttatccc ctttaacaga
      481 gctttttttc cactcgttct gcgcattttc gatgcgggtg tacgcctcgg caaaattgtg
      541 attgcccgct gtggccatgc ccgcgtaaat ggtgtagtcg agccagtaga ttttcccttc
      601 gtctttttct ttaagttgaa gaaaaaggcg ctcggcgttt gaatactctt cgttttcaaa
      661 atagctgagc gcaatgtagt tgagcgcgta gaattgcagc ttctcgtctt ttgcttcggt
      721 taaggggatg agttcgttct tagcttgcgg aaaacgtcca ccttcataat acatcttacc
      781 gatttgaaaa tgcacccagt cgagcttttc atattcagga aaaagaaaaa tgactttttt
      841 gtattcggtg atggcgcggt agtagtcttg ttcgtcggcg aatcgatcgg ccaagttcaa
      901 aaaggttttg ggtttggcaa agtccggctt cgccgcagga gcggcggata aactttggag
      961 tgcaatgagc gccgccgtcg agagcaacat ataacgaaga aaaaaatagt gaggtaaaaa
     1021 tttcatagtg tgtgtagatg ttgttaataa gtgaccggtg gatcgtacgg gtattctttt
     1081 tggttgggat gacagcggat gagacgatct gtccccatga caatcccctt aaagagacca
     1141 tatttctgca ctgcctcggc tgtgtattgc gagcaagtgg gtatataatg gcaggttgcc
     1201 cccgaaagag gactgatgta tttttggtag aaacgaatga gtgtgttaga gtcgctcatt
     1261 aaggatatgc gttgatgggg ttgaggcaag atggcgtctt gggttactgc atgccgcatc
     1321 gagatgctcc acggttcttc ggcgtgtagc gtgcaaagca ttgtgcttaa aaccccaaaa
     1381 agaatatagc gagttttata aatcatacgc cctgaaaaac ttgtgatgca ctcgtaaaac
     1441 tttaagtttg agcacaagct acaaagaacg actggttaga caaacgagtc tgtgtgccaa
     1501 tcgtaaaatt tgatactgca ttcaatttcg attgcatcta taccgatatt ctaaagggaa
     1561 acctctatgc gacgtatgtt aatcatcatg gcgattgccg tcttttcgat gccgtatctt
     1621 tatgcggtac cgtttacttg ggaaagtaaa gccgacgaac ctggattcgt tgtcgacgtt
     1681 ccggcaagtt ggagcaaagc atcgcgcgca cgcgatggtg tcggcaatgt acattttgaa
     1741 aaaaaggaaa aaacaggacg tgtcgccatt gaagtgcgcg catactcgac agaagatacc
     1801 gatattgatc aactggtttt gcaactgcgc acgcgccttg ccgtcaaata cgaccggttg
     1861 tatttacaaa aacgccaaga gatgacattc cgtaaaggcg ttgaaaagca aatctggaat
     1921 gcccgcgtcg gcaaaataaa atatgctctc acgacagcgt ttgtcgttgc agataatcga
     1981 gttttacaaa taacgtgcat tgcgccggtg agccaagcga aaagattcac aatccttttc
     2041 gataacgcac ttctttcgct cgactttggc gatggcagcg cggcagaacc cgcaagcgca
     2101 gaagcgccgg cagaaacccc cgcggctacg ccagtggccc ccgaatccgc tgtgaatcca
     2161 ccggcagccc ctagcggcaa accccaacca ccagttattc aattttaacg gaaaagaaag
     2221 aggcaaagca tgaaaaaatt acgtcactat tttatactcc ttatactaat atttcctttg
     2281 atgctcaccg cagaggatac accctctggc gcggatgaac tcgaagagct gaaagcgatg
     2341 gcaaaagaga tgagcgactt gtggggcgag agggtgtgcg cgagcgatgt tacgcaccgt
     2401 tacgaagaaa cggtggtgtc gattgcttgt aacgccgagt accaaagcgc cgagcgcgac
     2461 cgtatgctcg attatctttt taagaaagga tattttttta gaggtgaatc gtacaatgtg
     2521 aaaggtacga ccggtcatta ttggtataaa ctcggcaaaa aagaaggctc acgcactcgt
     2581 aatttattta tccgtctgca catcacgagc caaggtgcgc tgtggggttc ggtaaaacca
     2641 cccacccaaa ttgcgcttta tgtggattat ctctcgagca gcgacgaact cacagcatgg
     2701 caaactttag gcgttccggt gacttttggc ctcaaagtga gcgaaggtgc gaaagaactc
     2761 ctgcaacagc ttgaagaata taaacaagaa gcgtgggtgt ctttagattt gcgccaaggt
     2821 acattttcag aacccgatca aaccgcatcc atccgcgaca tcattgaaca ggatttaatc
     2881 gctccgcata ttaaaaatgc tatcgagtcc accggtgaag tgatgggttt tgtcatgcgc
     2941 gatttgaata acatcaccac gacggtcgcc tcggcgcgcg ctatttttaa cgcaatgaaa
     3001 gccgaaggta aaacaatggt gcttttgccg gcaagacaca atgccgcgct ttccaccacg
     3061 gcgaatgtaa tggggatgaa taaccgtgcg gtgacgtatg atatgacaat gatgtgtaaa
     3121 aaagacccag gtaaaatttg gtcgtttttg aaatcaaaga ccaatgcggg gggcatcatt
     3181 gtccgctttc aggcaaaaga taaacgttgt gcgtatcgat tgagccgcac gatgcgccgc
     3241 gatggtaagt tagaatataa gaagctcagt agtatttttg gttacaaccc cgcgcgtacg
     3301 gaaggtcgcg gtgactaata atcagctata gagacggaag cgtctttata gctccaaaca
     3361 ttatttaatt tttagaatac gttaccggta cagtaaaaga gcccgttgaa agtgtaaccg
     3421 tgcggccttt gaattggcct tctgcaaaac gcacagtgcc accaaatgac cctgttagtt
     3481 gagtaatctt accgtcaaca agggtggggg ttaagtttac ttgaaaaaaa gtatctctgt
     3541 attgttcttc acgcatgacg ccatcgccag tgcggagccg ttcttcgcgg gttttattaa
     3601 cccaagttgg aggttcttgc acgtattcaa cacgtatctt ctttcgtttt ttacgttcgg
     3661 ttttatgccg tttttcgcaa cgttcataac ttgctttatg tgccgccgtc tgcagttgta
     3721 aggtgttgtg cattgtgcgg cattcgggtg ggagattatc cgaggtatcg ttagggtcgt
     3781 cctccatctc tccggtttcg acttctttgc gacgattata gcgcataaaa tcgtcgcgtg
     3841 caaaggttat ctcgggcacc acgctgtaca taccttttgt tgtgagtact gttagagaaa
     3901 tactgttgct gctggtggtg aactgctgtg gggtcgtaaa cataggggat tctacggtta
     3961 gagcaaattg tacccgtgca gtgcgatcag tcacgccaat aatgagttgg gatttatccc
     4021 ctttttgaat gaaagcagtt tttgcatcgg caagcgagta ggttttgcca tctaaggtaa
     4081 acgagatggc tccctctgca aaaagaggag atataaagaa agaaaataaa aataagttaa
     4141 atataaaaag gcgcataaag tacctactac tatatgttgt tttaaggaaa aagagtgcca
     4201 tttttgttga gaactaagag cctgtgcgtc ccgcaagttt tatggccaga ctggaactca
     4261 ctaaggccaa cccaagaggg taaatttcgc ctcagtcaat ttctacctgg tcgcctttca
     4321 tacgcttgta gaaggtgttg cgtaacaggt cacgaagtta ccaactggta ttcacagcgt
     4381 tgggggtgag gagcgaaacc cgaaagtgct tccacgccgc tttcccttgc ccgatagtcg
     4441 ctcccatgtc ggatagagaa cggctcaatc caccgtcgcc gtcggaac
//
LOCUS       DAOMPD010000195         4457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8162_length_4457_cov_2.459791, whole genome shotgun sequence.
ACCESSION   DAOMPD010000195 DAOMPD010000000
VERSION     DAOMPD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8162_length_4457_cov_2.459791"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1012
                     /locus_tag="PLY93_07790"
     CDS             98..1012
                     /locus_tag="PLY93_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRP69416.1"
                     /translation="MKKLILFTAAVAMIAIGCGEKKEQATLDTAKFTYVKGDVLVSGK
                     PATLGQTVSKDATIEVKSNSMAVLQFANAASITLKANSALSIANLSKNDSGKPVIELS
                     QSSGSSFSKIAKGQSEFSIKTPTAVAGVRGTSFELTVGNGKVTQIKLLEGKVAVTKAD
                     ATPAELENAVVVDAGQKIEATTVAISKPVEISEAEKSSLQALNTVEIAPASGQVVVPE
                     SVEKYATGAAASASLAEAATPVEKPVVKQLTLADIKAKYGRVAKIETKNGKTYYGYFN
                     QAGANMTIQTVNGQVSIPVASVQKVTPQ"
     gene            1377..3557
                     /locus_tag="PLY93_07795"
     CDS             1377..3557
                     /locus_tag="PLY93_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69417.1"
                     /translation="MLKRFIFSLLFLGGFTAHLSALTEQEEKHAALRLDQAFNAYYTE
                     NFVEATKAFEEYLKIKGENEVPLRYLARIALTTGDATSAAKYLERAIKINPPAQPSLQ
                     LLSEIYLKQNKYNDAIRILHLILKNDPFNERALQVLAYIYQQKQEMRQAATYYKRLII
                     AVQKGSGNSDLLAQSLYFLGNYYYQQDNFQRSLVYFNKLHELDTENARYLLIIGELQK
                     ITGQFKASVATHEKLITEQPKFSAAWESLAETYYILDNSKAQRAIEQYKTIKPKNVPD
                     LLHAIELQLTGKDESALKAFDKVLQENQNRLSARIGRYRIFNARFKALPQDTPKETMD
                     AKALELRNEAFAVVVIAQRLNAYSMAREYAHKTLALLNAQADEIKFREKFYVKKSAPV
                     SHELLTSQMEQLAIDFIELYTTHASTLDSLGERKAAATYQELAAKVILELQSFIDTAL
                     KDPTNQNNPEKKAALEKRAREAKNQLYQTRVSQAWTQLNIPGSVDAAEKTVDTAIQIE
                     NEYATAYFVKGLIYMTSAQKEKSHYQKAAAYFEKAVRITEEKSKKKVAPANYYFNWGM
                     ALEKFADFSEAEKQLQRAIELDAYNPTYLNYLGYMYSLRNIRLEEANSLVLRALEDDP
                     ENEAYLDTFGWIEFKLGHHREAIEQLSVAASFAQKKPPVDVVIYFHLAEVHAKLNNRI
                     TAIEYYQKTLAGIEKASEPLDAEYIRAQIKKLESEHKETKNQEK"
     gene            3573..>4457
                     /locus_tag="PLY93_07800"
     CDS             3573..>4457
                     /locus_tag="PLY93_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69418.1"
                     /translation="MKYKDFDEFRDAYKKRALSSEFKKAGLETMEKLHNEILDKPIKE
                     WLAFAADISPDRNGESEWRRALSAISKNIRAKSQEIIVKNGRTGRDILEQASINAWKN
                     VAQHPIDIPSDTVREIMNQKAVRELFLNVVNDSINGFTKSIKLPIVGDLNKVVDDKMI
                     KAFLMPFMDSIVKIATDFVNNKSNQPMMSEFTGQLLEIALDQKPDILNVLMTDELEDK
                     IIAATYATAADKTFRTRAHEHVLDVIEAMRKDAGDKTLREWLKERDRSLPFSAPTDKE
                     LEHAQKILSRLACTASSNT"
BASE COUNT         1454 a         1065 c          932 g         1006 t
ORIGIN      
        1 tacaaccgtc ccgaaaaaat tgcatttttt accggacact ttgagtcgaa gttgtaagat
       61 ataagtgaga cgagaataat ctggtatagg aggattaatg aaaaaactca tactcttcac
      121 agcagcggtt gcaatgattg ccatcggttg cggtgaaaaa aaagaacaag cgacattaga
      181 tacggcgaaa tttacctacg tcaaaggtga tgttttggta agtggcaaac cggcaactct
      241 cggtcaaacc gtgagtaaag acgcaactat tgaagtaaaa agtaactcaa tggctgtatt
      301 gcaatttgct aacgctgcat cgattacgct aaaagcgaat tcagcattgt caatcgccaa
      361 cttgagcaaa aacgacagcg gcaaacccgt tatcgaactt tctcaatcga gcggtagctc
      421 tttcagcaaa attgcgaaag gccaatctga attttcaatc aaaacaccaa ctgcggttgc
      481 gggtgtgcgc ggtacatcgt ttgagttaac cgtcggtaac ggcaaagtaa ctcaaattaa
      541 actgctcgaa ggtaaagttg ccgttacgaa agcagatgca acaccggctg agttagaaaa
      601 tgcagtcgtt gtcgacgcgg gtcaaaaaat cgaagcaacc actgttgcga tttctaaacc
      661 tgttgaaatc agcgaagcag aaaagtcaag cttacaggct ttgaacactg ttgaaatcgc
      721 tccagcaagc ggccaagttg tcgtacccga aagcgttgaa aaatatgcaa ccggtgcagc
      781 agcgtcggca tcactggctg aagcagcaac tcctgttgaa aaacctgttg taaaacagct
      841 cactctcgca gacatcaaag ctaagtatgg tcgtgttgcg aaaattgaga ctaaaaacgg
      901 aaaaacctac tacggttact ttaaccaagc aggcgctaac atgactattc aaactgtgaa
      961 cggtcaagtc agcattccag tggcaagcgt acaaaaagta acgccacaat aaacttgact
     1021 tgagccgatt accgagtgat tcgctgctca actcgatacg aaaacctcag catagctgag
     1081 gttttcactc ggtgaccgct cttacggttt ctgtcctcag agagcgaaga cgctcacgat
     1141 acgaaaacct cagcatccgc agaggttttc actcggtgac cgctcttgcg gtctctgtcc
     1201 tcagagagca aagacgctca cgatatgaaa acctcagcat agccgaggtt tccactcggt
     1261 gaccggtggc gaattatatc gaggtgacgc gaagtgcccg ctcgttgagc gggctttttt
     1321 tatatattaa gaaaacgctt gccgctaaga atgccactgc aagcaatccc tgacgcatgc
     1381 ttaaacgttt tatcttttct ctcctttttt tgggaggatt cacagcacat cttagcgcac
     1441 tgaccgaaca agaagaaaaa catgcagcac tccgtttgga tcaagctttc aatgcttatt
     1501 acaccgaaaa ctttgttgaa gcaactaaag cgtttgagga atacttaaaa attaaaggcg
     1561 aaaacgaagt gcctttgcgc tatttggcac ggattgctct cacaacaggc gatgcgacaa
     1621 gtgcggcaaa atacttagaa cgtgccatta aaataaatcc accggcgcag ccttcactcc
     1681 aactactttc tgaaatttat ctaaaacaaa ataaatacaa cgacgcaatc cgtattttac
     1741 acttaatctt aaaaaacgat ccatttaacg aacgtgcttt acaagtgctg gcttatattt
     1801 atcaacagaa acaagagatg cgccaagccg cgacgtacta caaaagactc atcatcgccg
     1861 tgcaaaaagg aagcggtaac tccgatttac tcgcacaatc tctttatttt ttagggaact
     1921 actattacca acaagacaat tttcagcgtt cgttggtata cttcaacaaa ctccacgaat
     1981 tagatacgga gaatgcgcgc tatctactca tcatcggtga attacaaaaa attaccggcc
     2041 aattcaaagc gtccgttgca acacatgaga aactcattac cgagcaaccc aaattcagcg
     2101 ccgcgtggga gtcattggcg gagacgtact atatcttaga taactctaaa gcacagcgtg
     2161 ctatcgaaca gtacaaaaca attaaaccta aaaatgtacc cgatctttta cacgccattg
     2221 agttacaact caccggcaaa gacgaaagcg ccttaaaggc gttcgacaaa gtgttgcaag
     2281 aaaatcagaa tcgtttaagt gcgcgtatcg gtcgttaccg catttttaat gcgcgtttca
     2341 aagcgcttcc ccaagatacc cccaaagaaa caatggatgc caaagccctt gagctgcgca
     2401 acgaagcgtt tgcggttgtt gtcattgcgc aacggctaaa cgcatactcc atggcacgtg
     2461 aatacgcgca taaaactctc gctcttttaa atgcgcaggc ggatgaaata aaatttcgcg
     2521 agaaatttta tgtaaaaaaa tctgcgcctg tatcgcatga attattaaca tcgcagatgg
     2581 aacaactcgc gattgatttt atcgaacttt acaccacgca cgcttcgact ttagacagtt
     2641 tgggcgagag aaaagcggcc gccacctacc aagagttggc ggctaaagtt attttagagc
     2701 tacagagttt tatcgacact gcactcaaag atccaacaaa tcaaaataac ccagaaaaaa
     2761 aagccgcact tgaaaagcga gcgcgagaag ctaaaaacca actctaccaa acacgcgtca
     2821 gccaagcatg gactcagctc aacattcccg gctccgtaga cgccgcagaa aaaaccgtcg
     2881 ataccgctat ccaaatcgaa aacgagtacg cgaccgctta ttttgtaaaa gggcttatct
     2941 acatgacctc ggcgcaaaaa gaaaaatcgc actatcaaaa agccgccgcg tactttgaaa
     3001 aggcagtccg catcaccgaa gaaaaaagta aaaagaaagt cgcgcctgct aactattatt
     3061 tcaactgggg catggcgctt gaaaaatttg cagatttttc tgaagcggaa aagcaactcc
     3121 agcgcgccat cgaactcgac gcgtataatc cgacatacct caattatttg ggatacatgt
     3181 actcgctacg caacatccgc ttggaagaag cgaactcttt ggtactgcgt gcattagaag
     3241 acgatcccga aaatgaagcg tacctcgaca catttggctg gattgagttc aagttaggcc
     3301 accaccgtga agcgattgaa cagctcagcg tcgccgcatc ttttgctcaa aaaaaaccgc
     3361 ccgtcgatgt cgtcatttat ttccacttag ccgaggtgca cgccaaactc aataaccgca
     3421 tcactgcgat tgaatactac caaaagacat tggctggtat cgaaaaggcg tcagagcctc
     3481 tcgacgctga gtatatacgc gctcaaatta aaaaattaga atcggaacac aaagaaacta
     3541 aaaatcaaga aaagtaattc caaggagaaa aaatgaaata caaggatttt gacgaatttc
     3601 gggacgccta caaaaagcgg gcattaagca gtgagtttaa gaaagcgggt ttagagacca
     3661 tggagaaact ccataatgaa attttagaca aacccatcaa agaatggtta gcgtttgccg
     3721 ccgatatttc ccccgacaga aacggtgaaa gcgaatggcg tagagcgctt tcggctattt
     3781 caaaaaatat ccgcgcaaag agccaagaaa tcatcgtcaa aaacggacgt accggtcgcg
     3841 acatcctcga acaagcatcg atcaacgctt ggaaaaacgt cgcccaacac cccatcgata
     3901 ttccatcgga caccgtgcgg gaaattatga accaaaaagc ggtgcgcgaa ctttttttaa
     3961 acgtggtcaa cgactcaatc aacggtttta caaaaagtat taaactacct atcgtaggcg
     4021 acctcaataa agtcgtcgac gataaaatga ttaaagcctt tttgatgccg ttcatggatt
     4081 ccattgttaa aattgcgacc gactttgtga ataataaatc caaccaaccg atgatgagcg
     4141 aatttaccgg ccaactttta gaaatcgcgc tcgatcaaaa gccagacatc ttgaacgtac
     4201 ttatgacaga cgaactcgaa gacaaaatta tcgccgcaac gtacgccact gccgccgaca
     4261 aaacattccg cacacgcgcg cacgaacacg tcctcgatgt catcgaagcg atgcgcaaag
     4321 acgcgggcga caaaacgctg cgcgaatggc ttaaagagcg tgatcgctcc cttcccttta
     4381 gcgcacccac agacaaagaa ctggaacacg cacaaaaaat tctttcgcgt ttagcttgta
     4441 ccgcttcgag caataca
//
LOCUS       DAOMPD010000196         4449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8179_length_4449_cov_2.711652, whole genome shotgun sequence.
ACCESSION   DAOMPD010000196 DAOMPD010000000
VERSION     DAOMPD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_4449_cov_2.711652"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLY93_07805"
     CDS             complement(<1..809)
                     /locus_tag="PLY93_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69419.1"
                     /translation="MPLKFESLVTLWQQYQIYFILSFVLVGLTLIAVVTTVIALRKAR
                     RLKTPATNTALQGKSHLAPSEKKTQNENATPHGIKRTKLAVGDVANPWTQAVYERHSP
                     EGKIDALLPPFAFDEHGGIHRLAFPDAVKGIDIERALQYTDTAAGLAVNGAYAVFLLW
                     HAGDARYAAYALKVASHAQIAPEQKNLVRFLFQDVIEKRVSIFDVPGIERWSKPAFRA
                     AVTLYTAPKISEERGGELEKVLPTALSRFYELTLAQKLPIKIIYRKAATNP"
     gene            complement(814..3225)
                     /gene="lon"
                     /locus_tag="PLY93_07810"
     CDS             complement(814..3225)
                     /gene="lon"
                     /locus_tag="PLY93_07810"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRP69420.1"
                     /translation="MTEITPARFPLLPLRDMVIHPGSVTSIFVGREKSMEATRRAMEL
                     DRKLCLATQRSAEIVLPQLGDLHEKGVIVEILQLLKMPDNTYKILVEGLYRARLVDVD
                     DSDEKCQVAVVMPASSVFPEGDKEEHLYQKSIAKLTDAFERINDQQVFMATEVWRELR
                     AKNDTERLIHAVAESLKIPFVEQQDILATDNVWERAEKVRAAMENQLELSRLEGQIHD
                     KVRAQLDKMQRQYYLQEQIKVIREEMGETEPDEFETLRKRTKEKSWSDEIRQKTESEI
                     RKLEKTPPLSAESGVIRNYLDWLLDLPFSETTQDSDDIARAQKILDESHYGLEKIKDR
                     ILEFIAVKHLSSQIRGPILCLVGPPGVGKTSLAKSLAESLDRKFVRIALGGVRDEAEI
                     RGHRRTYIGSMPGRIIAALKKIKVKNPVVLLDEIDKISSDFRGNPAAALLEVLDPEQN
                     KEFVDHYLDVPFDLSQVIFVATANVTHSIPDALLDRLELIQLSGYTEMEKIQIANKYL
                     IPRQIEENGIRDVELTYTNKSLAYILRHYTKEAGVRQFERELAQIARRIAREVVEQKS
                     NEAKVLDKQSIIRYLGPAKYLYTRKENENPIGKVSGLAWTSAGGDILNIEAALAYGKG
                     KLTLTGNLGDVMKESANAALGFVRSQASMLGLSTDFFEKNDIFIHVPEGAIPKDGPSA
                     GITLVTAIISALTQRGVYSHIAMTGEVTLRGNVLAIGGLKEKVLAAFRGGITHVICPK
                     DNEKDFNEIPQSIRQQMQFHFVGHVREVIALALVQGGTVFQEKAGLAFYATHLAPGAS
                     MTQPM"
     gene            complement(3252..>4449)
                     /gene="clpX"
                     /locus_tag="PLY93_07815"
     CDS             complement(3252..>4449)
                     /gene="clpX"
                     /locus_tag="PLY93_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRP69421.1"
                     /translation="EAAQKLPEPAEIKKILDEYVIGQDAAKRALSVAVYNHYKRIQYD
                     REQAKKNGGKFARKHQATDTSGVEIDKSNVLLIGPTGSGKTFLAQTLARILQVPCALV
                     DATALTEAGYVGEDVENILLRLYQNADANIEKAQTGIIYIDEIDKIARKSDNPSITRD
                     VSGEGVQQALLKIIEGTVASVPPVGGRKHPHQDFIQMDTRNILFICGGAFEGLDKVVQ
                     KRVEKSTLGFDFKAPKRKEQTSEYLRLTTPDDLMKYGLIPEFIGRLPIIRSLDNLDET
                     AMVKILTEPRNALVKQYKKIFELEGIKLTFEDDAVKAIAQKALTRQTGARGLRAILED
                     AMMDLMYEIPTMIGVSEVVITRGMIESGEKPRIVTRAETDKTRKTKQKDAVQDDNYLL
                     PPEKMA"
BASE COUNT          993 a         1098 c         1094 g         1264 t
ORIGIN      
        1 ataggatttg tggcagcttt acgataaatg attttaatgg gaagtttttg tgccagtgtc
       61 agttcataaa aacggctcaa ggcggtgggt aatactttct ccaattctcc gccgcgttct
      121 tctgaaattt taggtgcggt gtacaacgtg accgctgctc ggaacgcagg cttcgaccaa
      181 cgctcaatac cgggcacgtc aaaaatagaa actctttttt caatgacgtc ttgaaataaa
      241 aaacgcacta aatttttttg ttcgggggca atttgtgcgt ggctagcgac tttgagcgca
      301 tacgctgcgt aacgcgcatc gcccgcatgc cacaacaaaa ataccgcgta cgcgccgttg
      361 accgccaaac ctgcggcggt atcagtgtat tgcaacgcgc gctcgatgtc gatgcctttc
      421 accgcatcgg gaaacgccag ccggtgtata ccaccgtgtt catcgaacgc aaaaggcggc
      481 aacaacgcgt caatttttcc ttcgggcgaa tgccgctcgt acaccgcttg cgtccacggg
      541 ttggccacgt cacccacagc aagctttgta cgcttaatgc cgtgtggtgt ggcgttttcg
      601 ttttgtgtct ttttttcgct tggcgccaag tgggatttac cctgcagcgc ggtgttggtt
      661 gccggtgttt tcagccgccg tgctttgcgt aatgcaatca ctgtggtgac aacggcaatc
      721 agggttaatc ccaccaaaac aaacgaaaga ataaaataga tttgatattg ttgccaaagc
      781 gtaacgagcg attcaaactt caacggcatc gcatcacatg ggttgtgtca tcgacgcgcc
      841 tggagcgagg tgtgtcgcgt agaaggcaag ccctgctttt tcttgaaaaa cggtgccacc
      901 ttgtaccaag gcgagtgcaa tcacttcgcg gacgtggcct acaaaatgaa attgcatttg
      961 ttggcggatg ctttgtggaa tttcgttaaa atctttttcg ttgtctttgg ggcaaatgac
     1021 gtgagtaatg ccaccacgaa acgccgcgag cactttttct ttgagcccac cgatcgcaag
     1081 cacattgccg cgcagcgtca cttcgcccgt cattgcgatg tggctataca cgccacgctg
     1141 cgtgagtgct gaaatgattg ccgtcaccaa cgtaattccc gccgacgggc cgtccttggg
     1201 gattgcgcct tcgggtacgt ggataaaaat atcgtttttt tcaaaaaagt cggtactaag
     1261 acccaacatc gatgcctgcg agcgcacaaa ccccagcgca gcgttcgccg attctttcat
     1321 cacgtcgcct aaattgccgg tgagagtgag cttacctttg ccgtacgcca acgccgcttc
     1381 gatattcaaa atgtcgccgc ccgccgatgt ccatgcgaga ccggaaactt taccgatagg
     1441 attttcgttt tctttacgag tgtataaata tttcgccggg cctaaataac gaatgatgga
     1501 ttgtttatcc aataccttcg cttcgttcga tttttgctca accacctcgc gtgcaatgcg
     1561 gcgggcgatt tgtgccagct cgcgttcaaa ttggcgcacc ccggcttctt tggtgtagtg
     1621 gcgtaaaata tacgcgagcg atttattggt gtatgtcaat tctacgtcgc ggatgccatt
     1681 ttcttcgatt tgccgtggga ttaaatactt gttcgcgatt tgaatttttt ccatttcggt
     1741 atagcccgaa agttgaataa gctccagacg atctaaaagc gcatcgggga ttgaatgcgt
     1801 cacgtttgcg gtggcgacaa aaatcacttg cgataaatca aagggcacgt cgaggtagtg
     1861 atcaacgaac tctttgtttt gctcggggtc gagcacctca agaagcgccg ccgccggatt
     1921 tcctctgaag tcggaagaga ttttatcgat ttcatcgaga agcaccaccg gattttttac
     1981 tttaattttt ttaagggcgg cgataatacg tcctggcatc gaaccgatgt aggtgcggcg
     2041 gtggccgcga atttctgctt catcgcgcac accgcctaaa gcgatgcgca caaatttacg
     2101 atctaggctt tcggcgagcg atttagcaag gctggtttta cccaccccgg gtgggcccac
     2161 aagacataaa atggggccac ggatttgcga cgacaaatgc tttactgcaa taaattctaa
     2221 aatgcggtct ttgattttct ctaaaccgta atggctttca tccagaattt tttgtgcgcg
     2281 cgcaatgtca tcgctatctt gcgttgtttc tgaaaaaggt aaatctaata gccaatctaa
     2341 ataattgcgg atgacgcccg actcggcgga caaaggcgga gttttttcca gtttgcgaat
     2401 ttcggattcg gttttttggc gtatttcatc gctccagctt ttttctttcg tgcgcttacg
     2461 gagcgtttca aactcgtcgg gttcggtttc acccatttct tcgcggataa ctttaatttg
     2521 ctcttggaga taatactgcc gctgcatttt atcgagctgc gcgcgcacct tatcgtgaat
     2581 ttggccttcc aaccgcgaaa gctctaactg attttccatc gccgcgcgta ctttttcggc
     2641 tctttcccaa acgttatcgg tggcaagaat atcttgttgc tcgacgaatg gtattttcaa
     2701 actttcggca accgcgtgga ttaaacgttc tgtatcgttt ttggcgcgta gctcgcgcca
     2761 cacctcggtc gccataaaaa cttgctgatc gttgatgcgc tcgaacgcgt cggtgagttt
     2821 ggcgatcgat ttttgataga gatgctcttc tttgtcacct tcgggaaaaa ccgacgatgc
     2881 aggcatcacc accgcaactt ggcatttctc gtcgctatcg tcaacatcaa ctaagcgcgc
     2941 gcggtaaagc ccttcgacta aaattttata cgtgttgtcg ggcattttga gtaattgcaa
     3001 aatttcgaca atcactcctt tttcatgtaa atcccccaac tgcggtaaaa caatttcagc
     3061 gctgcgctgc gtcgccaaac aaagcttgcg gtcaagctcc atcgcgcggc gcgtggcctc
     3121 catcgatttt tcgcgcccaa cgaaaatcga tgtcacgctg cctgggtgaa tcaccatgtc
     3181 gcgcaaaggt agtagtggga aacgagcagg ggtgatttcg gtcatgcggt tgtgcctttc
     3241 cttattatta cttatgccat cttttcggga ggcaataaat aattatcgtc ttgtaccgcg
     3301 tctttctgtt ttgttttcct tgttttgtcg gtctccgcac gggtgacaat gcgtggcttt
     3361 tcaccgcttt caatcatacc acgggtaatg actacctcgc tcacgccaat cattgtgggt
     3421 atttcgtaca tgaggtccat catcgcgtct tcgagaatag cacgcaagcc acgagcgccg
     3481 gtttggcggg tgagcgcttt ttgagcgatt gctttcaccg catcgtcttc aaaagtcaac
     3541 ttgatgcctt cgagctcgaa gattttttta tattgtttaa ctaatgcatt acgcggctct
     3601 gtcaaaattt tgaccatcgc cgtttcgtcc aagttgtcga gcgaacggat aatcggcaag
     3661 cgaccgatga actcggggat aagcccgtac ttcatgaggt cgtcgggtgt ggtgaggcgc
     3721 aagtattcgc tggtttgttc tttgcgcttg ggcgctttga aatcaaaacc gagtgtcgat
     3781 ttttcgacgc gtttttgcac aactttatcc aagccttcaa acgcaccgcc gcaaataaaa
     3841 agaatgttgc gcgtgtccat ttggataaag tcttggtgtg ggtgtttgcg cccaccgacg
     3901 ggaggaacgc tcgccaccgt accctcgatg attttgagga gagcttgttg caccccttcg
     3961 cccgaaacgt cacgcgtgat cgaggggtta tccgacttgc gcgcaatttt atcgatttcg
     4021 tcgatataga tgatgccggt ttgagctttt tcaatgtttg catcggcgtt ttgataaagg
     4081 cgtaagagga tattttccac gtcttcgccg acataacccg cttcggtaag cgcagtagca
     4141 tcgacaagcg cacacggcac ttgtaaaatg cgcgccaagg tttgcgctaa aaatgttttt
     4201 cccgaaccgg tgggaccaat gagaagtacg ttcgatttat caatttcaac gcccgaagta
     4261 tctgtcgctt gatgcttacg cgcaaacttt cctccgtttt ttttcgcttg ttcgcggtcg
     4321 tattggatac gtttgtagtg gttgtagacc gcgaccgaaa gcgcacgttt ggcggcgtct
     4381 tggccaatca catattcgtc gagaatcttc ttaatttcgg cgggctcagg cagtttttgt
     4441 gcagcttca
//
LOCUS       DAOMPD010000197         4434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8207_length_4434_cov_2.626170, whole genome shotgun sequence.
ACCESSION   DAOMPD010000197 DAOMPD010000000
VERSION     DAOMPD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8207_length_4434_cov_2.626170"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..641)
                     /gene="atpF"
                     /locus_tag="PLY93_07820"
     CDS             complement(123..641)
                     /gene="atpF"
                     /locus_tag="PLY93_07820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRP69422.1"
                     /translation="MVLLTSTSIDLLKVSPGLVFWTTVTFIFVLVVLWLFAWKPIIKG
                     LDARNEKVQDDLDSSRKLREQAEAMMADHKKHLDAAKDQALHIIDEGKRDAESIRHRI
                     LADAKKEADEIHARAVVDIVQAKTKAIKELEKEVIDMSVQIVSKVLNRDVSKEDHRNI
                     ILKELDQLKAGK"
     gene            complement(667..897)
                     /gene="atpE"
                     /locus_tag="PLY93_07825"
     CDS             complement(667..897)
                     /gene="atpE"
                     /locus_tag="PLY93_07825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRP69423.1"
                     /translation="MGSIIAIGAGIAAGLAVLGAALGIGRIGGSATEGISRQPEAASK
                     IQTAMIIAAALIEGVALFALVIAFIMVNKITG"
     gene            complement(1008..2075)
                     /gene="atpB"
                     /locus_tag="PLY93_07830"
     CDS             complement(1008..2075)
                     /gene="atpB"
                     /locus_tag="PLY93_07830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRP69424.1"
                     /translation="MNKKKVVTLSFLIAALFAAPSFYAEGTVAVHADVAKTGAPQGAT
                     TAPAQHSITTESAAVEASPSAAHASTDFNVAETIAHHLSDAPLWKLELSGYDISITKR
                     VMMLFIGAFLLCALLIPLARRIAKDPYKKPTRFSGFVEVLINFIRNDVAHASLGHHSR
                     PYEPFLLTLFFFILFANVLGLIPSLGEIYSTIGQAFGFVQASGGHDSLHLPLPEKLWP
                     GITATGDIAVTSTLAVITLLVLFISGFAYQGPLFVRNIVPNGIPLLLWPIMWPIELLG
                     MFTKAFALAIRLLANMTAGHMILIVLLGFIFQFQSYLIAPVSVAASVAIYLLEIFVAF
                     LHAYIFTFLTSLFIAQVQHRH"
     gene            complement(2104..2376)
                     /locus_tag="PLY93_07835"
     CDS             complement(2104..2376)
                     /locus_tag="PLY93_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69425.1"
                     /translation="MTWLVISFAAGVLMSVGAAFVVRRLMHSQTGAGWLFYATIPMRV
                     LALLGGAMALLVIAPPHKEWLLLSYAGGLVVAGGVHLVVALSVLRK"
     gene            complement(2443..3777)
                     /locus_tag="PLY93_07840"
     CDS             complement(2443..3777)
                     /locus_tag="PLY93_07840"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP69426.1"
                     /translation="MNSTITDFIESATESELKDELMRYASLFAINEALSSTLEYEEVL
                     KLVLTQMRVLSGAENASIFLINKKEKVLEFAATTDAQAELLKTIRIPLGKGIAGYVAE
                     TGEYVNQSDIKNDERYYKEVDVTRGGETRSYLCVPLKLRGEIKGTVQLMNKESGEAFN
                     EADVRLMLNFASQAAMAIETTLSHRNALARKAFERDVHLAADIQKQALPLQMPEILGY
                     SFYGHTEPAQDVGGDYFQFIEHAERAGRKKIDIVVADVAGKGIPASLIVSNFHAALQL
                     LSPLYATLGELAFQLNNFMRKNLISGTFVTAFIARLDTQSGRMHYVGCGHNPPFLFSK
                     STNGVEIKELEQSGTAFGLRFDREYRVHALDLKEGDAIVIYSDGVTEAMNLHNELYET
                     ERMNARISKTLSAEPSSVDAQTIITELCDDVKSFRGGADVSDDLTVVCLKKE"
     gene            3868..4365
                     /locus_tag="PLY93_07845"
     CDS             3868..4365
                     /locus_tag="PLY93_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69427.1"
                     /translation="MRIFLFVGLTLLIAACAENTATNRAAPRAYFPTTMTVAAVSQSG
                     NTNTSQASLQMSNTDSKTSTLSGGAENTPFFLYYDMKAGTSGGQLDTSEVRMYYRGAL
                     TDKITVVGNVNGSASGAQKNRTAVLRQLHQQALQMKADGIYDVQYQDRGNTLYATGKA
                     FRFKE"
BASE COUNT         1222 a         1147 c          991 g         1074 t
ORIGIN      
        1 aaaccgcgca tcttcgcgca gcgcatcgcg caccagatgt aaatccgcct cggccgcttc
       61 ttggttcaaa ttagccacct gcgagagggc taaagcataa ttgtaagcgg cctttgacgt
      121 cactatttac ccgctttgag ttggtccaac tctttgagta taatattgcg atgatcttct
      181 ttgcttacat cgcggtttaa gactttagag acaatctgta cgctcatgtc gataacttct
      241 ttctctaact ctttaatcgc ttttgttttc gcctgcacaa tgtcgacaac agcacgcgca
      301 tgaatttcat cggcttcttt tttagcgtcc gctaaaatac gatgacgaat cgattctgca
      361 tcgcgcttac cttcgtcgat aatatgaaga gcctggtctt tagccgcatc gagatgcttc
      421 ttatgatcgg ccatcattgc ttcggcttgt tcgcgtagtt tgcgagaaga gtcaagatcg
      481 tcctgtactt tctcgttacg cgcgtccaag cctttaatga tcggcttcca tgcaaacaac
      541 cagagaacga cgagcacaaa aataaaagta accgtagtcc aaaagaccaa acccggcgac
      601 actttcaata aatcgatact cgtcgaagtc agcaatacca tgatgccctc aattacctac
      661 cgacgcttaa ccggtaattt tgttgaccat tatgaaggcg ataactaacg caaaaagcgc
      721 cacaccttca ataagagccg ctgcaataat catcgcggtc tgaattttgc tcgccgcttc
      781 gggttgacgg ctaatgcctt cagtcgccga accgccgata cggccaatac ctaacgccgc
      841 acccaacaca gccaaacctg ccgcaatacc cgctccaatt gcaatgattg atcccatagt
      901 ttactccttt caatagttat taatacgtga aagaacaagt ctttcatttt gcaaaactat
      961 ttctttcacg tattctctat acccccattc catacttaca tagcctgtta atgccgatgc
     1021 tgcacctgcg caataaaaag cgacgtaagg aatgtgaata tatatgcgtg caaaaaggcg
     1081 acaaatatct ctaaaagata aatcgccacc gaagcggcca cactgacggg cgcaatcaaa
     1141 tacgattgaa attgaaaaat aaatcccaag agcacaataa gaatcatgtg accggcggtc
     1201 atgttcgcca aaagacgaat cgcgagagcg aatgcttttg taaacatgcc caagagctca
     1261 atcggccaca taatcggcca caaaagcaac gggatgccgt tgggtacaat gttgcggaca
     1321 aaaagaggcc cttggtatgc gaaacctgaa ataaagagaa caagcagcgt tatcaccgcc
     1381 aacgtcgaag tgactgcaat gtcaccggtt gccgtaatac ccggccacag tttttcgggg
     1441 agtggtaaat gaagcgaatc gtgccccccc gacgcctgca caaagccaaa cgcttgccca
     1501 atcgtactgt aaatttcacc caatgaaggg ataagtccca acacattcgc gaacaatata
     1561 aaaaagaaaa gcgtgagcaa aaacggctcg taaggacgcg agtggtgtcc caggctggcg
     1621 tgcgcgacat cgttacggat aaagttaatg aggacttcga caaaccctga aaaacgcgtg
     1681 ggctttttgt aagggtcttt agcgatgcgg cgcgccagtg ggatcaacaa cgcgcacaaa
     1741 agaaatgcac caataaaaag catcatcacg cgcttggtga ttgaaatatc gtaaccagaa
     1801 agctccagct tccagagagg ggcatccgat agatggtgtg caatcgtttc agcaacgtta
     1861 aaatcagtcg aagcatgcgc cgcagacgga ctcgcctcga ctgcagcaga ctcagtggtt
     1921 atagaatgtt gcgctggcgc ggttgttgcg ccttgaggcg cgcctgtttt agccacatcc
     1981 gcgtggacag ccaccgttcc ttcggcataa aacgatggag cggcaaagag cgccgcgatt
     2041 agaaatgaaa gagtgacaac ctttttctta ttcattctct taagttcatt ttttaaaata
     2101 atttcacttt cttaaaacac tcaaagcgac gacgagatga acaccccccg caaccacaag
     2161 cccgcctgca tacgacagca atagccattc tttgtgcggc ggcgctataa caagaagagc
     2221 catcgcccct cccaaaagcg ctaacacacg catgggtatc gttgcataaa acaaccaccc
     2281 cgcccccgtt tgcgaatgca ttaatcgacg cactacaaac gctgctccca ccgacataag
     2341 cacgccagcc gcaaatgaaa ttacgagcca agtcacctta tcgccttgtt gtgacggaag
     2401 gtttgcgttg tcgaaacata gcgacaagca aaatgtaatt ttttattctt tcttcagaca
     2461 aacaaccgtg agatcgtcgc tcacgtcggc gccgccgcga aagcttttaa cgtcatcgca
     2521 tagctcggta atgattgttt gtgcatcgac agaggagggt tctgctgaaa gagtctttga
     2581 gatacgcgca ttcatacgtt cggtctcgta tagctcgttg tgtaaattca tcgcttcggt
     2641 gaccccgtcg gaataaataa caatcgcatc gccttctttt aggtcgagcg catgcacacg
     2701 gtattctcga tcgaagcgta acccgaaggc agtgccgctt tgttcgagtt ctttgatttc
     2761 aacaccgttt gtgcttttgc taaaaaggaa cggcgggttg tgcccacagc ccacgtagtg
     2821 catccgaccc gattgagtgt ctaagcgcgc aatgaacgcc gtgacaaaag taccactaat
     2881 taaatttttg cgcataaaat tattgagctg aaacgccaac tcgcccaacg tggcataaag
     2941 cggcgacaac aactgcaacg ccgcatgaaa attcgaaacg atgagcgaag cagggatacc
     3001 cttgcctgca acatcggcga cgacaatgtc aatttttttc cggcctgctc gctcggcgtg
     3061 ttcgatgaat tgaaaataat caccacccac gtcttgtgcg ggctcagtgt ggccataaaa
     3121 cgaatagccc aaaatttcgg gcatttgaag cggcagtgct tgtttttgta tatcggcggc
     3181 gagatgcacg tcgcgctcaa acgctttgcg cgccaatgca ttgcggtgag acaacgtcgt
     3241 ttcaatcgcc atcgccgcct gtgaagcgaa attgagcatc aagcgtacat ccgcctcgtt
     3301 aaatgcttca ccgctttctt tattcatcag ttgcacggtg cctttaattt caccacgcag
     3361 tttcaacggt acacatagat acgaacgagt ttcaccacca cgagttacat ccacttcttt
     3421 atagtaccgt tcgtcgtttt tgatatcgct ttgatttaca tactcgcccg tttcagcgac
     3481 atagccagcg atacctttac ctagcggtat gcggattgtt ttgagcaact cggcttgcgc
     3541 gtctgttgtg gcggcaaatt cgagcacctt ttcttttttg ttgatgagga aaatggaagc
     3601 gttctccgca cccgagagca cgcgcatttg agtgagcact aacttcagca cttcttcata
     3661 ttccaaagtt gacgacaacg cttcgttgat ggcgaagagc gacgcatacc gcatgagttc
     3721 gtctttaagt tcactctcgg ttgcgctttc aataaagtcg gtgatggtcg aattcatggt
     3781 tagcgcactc ttttagagta agtgttcgtc atggcaataa aaaagtattt cttattgacc
     3841 ggtcaatacc gtgcttacaa aagcgccatg cgtatttttt tatttgtggg attaaccctt
     3901 ctcattgccg cttgcgctga gaacacagcg acgaatcgag cagcccctcg agcgtatttc
     3961 cccacgacca tgacagtggc agccgtttca cagagcggca acacgaatac atcccaggca
     4021 tctttacaaa tgagcaacac ggacagtaaa acttctaccc tatctggtgg tgctgaaaac
     4081 acaccgttct ttttatatta cgacatgaag gcgggcacat cgggtgggca gctcgacacc
     4141 agcgaagtgc ggatgtacta ccgaggcgcg ctcaccgata aaatcaccgt tgtcggcaat
     4201 gtgaatggga gtgcctccgg tgcacaaaaa aacagaacag cggtgctgcg ccagttacac
     4261 caacaagcgc tccaaatgaa agccgacggc atttacgacg tgcaatacca agacagaggc
     4321 aacacgctgt acgccaccgg caaagcgttt cgttttaaag aataactcat aaaagcctgt
     4381 atgtctaacc cacggacata cctgacaaat gctacgccgc aattgggggg tatt
//
LOCUS       DAOMPD010000198         4414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8242_length_4414_cov_3.339069, whole genome shotgun sequence.
ACCESSION   DAOMPD010000198 DAOMPD010000000
VERSION     DAOMPD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8242_length_4414_cov_3.339069"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLY93_07850"
     CDS             complement(<1..198)
                     /locus_tag="PLY93_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69428.1"
                     /translation="MLLLYGQRDLSGLTNFFQKVIYHQRALRLATSMFMEIKSQVSQL
                     FVGKADVFMQPTEITDTFGVNL"
     gene            298..1536
                     /locus_tag="PLY93_07855"
     CDS             298..1536
                     /locus_tag="PLY93_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP69429.1"
                     /translation="MLNFAMLSKTLLFSAIVFYLFSCKQPRDKQIEENVACAEFSPLV
                     HKKMHDWGKLFQTEVSFCESFPKTEGELQRLLQTADSKNISVRIRGRGHSMNGSTLPR
                     QGELLIVTENLRQYNWQSKEFIRVGGGISIYALQQWLKARGFSLPVFTDVLGPSVGGY
                     ISASGISLDSQKYGGFWNNVNRVRLVDAKGEMYVLTPKDKDFKWLFGSMGQLGVIVEA
                     DLKIVPYKKPRFRQEIGIIAESLVAGPKGQPRDTEYESSHLVWFTVIAPIEKKSELMT
                     DLLNIKAKYSDAINYIPEYIWPVRKLNFTPPLFYPENRELICIGIWGMPKSDNLNNNK
                     FLFGISEDISNLITQKKYYRRYIQSEVYKTKIDWREYWGEKIYSEFLQITKLYNPKGN
                     LNQNCIFGCDSTYIEPSTMP"
     gene            complement(1533..3029)
                     /locus_tag="PLY93_07860"
     CDS             complement(1533..3029)
                     /locus_tag="PLY93_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRP69430.1"
                     /translation="MSHFSLRRIVMLSSGKTINTPTLLLFCFFTGAIRGILEAIFFEP
                     QVFNSLMMLPYIPFYIAMFLGILILLKYVGGLSESRVYRVPLVALFLGLLPPILDIII
                     SGRPDTPFRYAYYLHVFTDIPLPSGFFVFFMPEQSVPMGEAITVWLTIAFAALYAYLQ
                     GANLLRTIFMLIAVYLLFFFHSYILPSLLQTWYTTKKTEFHNLELRLVLPLLQILAAV
                     FTYMLLRRISIKYILKRLLHIAPFMLLLAYGMLTANQSNTAIILFLILHFLLFFSMIV
                     QNDFFDQKEDRLQKRIIHFDAGDVFFFTAITVFTLILLAVAGKMYALFASLILVCGIL
                     YSFPFYRAKGRLFATMKFEGLWGALSYLGGLITLAPITSRISNETLVVLSIIFCGWSF
                     FSLFKDIKDVRTDYKTKNNSFYIWFYKKKWGLRKAHHFLMGIATLGLLVPTIYLFIQQ
                     RIILSVSLLCITILFFFIGYKIRLRHRTNALLAVLNIYLLFYLSQFYS"
     gene            3128..4270
                     /locus_tag="PLY93_07865"
     CDS             3128..4270
                     /locus_tag="PLY93_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69431.1"
                     /translation="MKLNSILAAVICLITLSCRDKDLGVKTAQKYCTNCHALPLPADL
                     DKESWGLVLDSMGTFIGIPSQNKSRPLFRAAANTVIPKRTITDSEWQALSDYFISGAH
                     QDEQPPKENAKMCNVSFQKTEIRIDSPVQAITWVELDAACNCLRFAEGIQGDLFEADL
                     KGKIRKKIHVGYSVTGSVEIHGKKYFVAAKNLLPNDDPSGILFTLNGQTPKILRNDLI
                     RPVYITSFTDNNKVPFLIISEFGHTQGRLSKINLTNYERTTLSDLSGYISSLINGKGE
                     LLALRAQADEAIVQFAPNIKNLIRFSPSWGSTSIAWTTFDAQNKEYLVTSHGDNGDLP
                     DMPYKPAHGIRVYENIDNNWAQKYFYPMPGAYKVMTGKFFEKQKTD"
BASE COUNT         1478 a          793 c          937 g         1206 t
ORIGIN      
        1 caaattgacc ccaaaagtgt cagttatctc tgtgggttgc ataaacacat cggctttacc
       61 cacaaacagc tgggaaacct gactctttat ttccatgaac atcgatgtcg caaggcgcag
      121 agctctttga tgatagatga ctttctgaaa aaaatttgtt aaaccactca aatcacgttg
      181 accgtataac agcagcatag ccagtgtaaa gagttctgta ccaaagtcta acgggatctg
      241 ggacggtatc ttcgtaatgt aattaagtgc ccgaagaagc tagagcatgg agggggtatg
      301 ctaaattttg ctatgttaag caaaacttta ttgttttctg caattgtatt ttacttattt
      361 tcctgtaaac agcctcggga taagcaaata gaagagaatg ttgcttgtgc agagttttcg
      421 cctcttgtgc acaaaaaaat gcacgattgg ggtaaactat ttcaaactga agtttctttt
      481 tgcgaaagtt tccctaaaac agaaggcgaa ctacaaagac ttctccaaac ggcggattca
      541 aaaaatattt ctgtgcgcat acgaggccgt gggcactcca tgaatggtag tactttgccg
      601 cgccagggag aactcctcat cgtcactgaa aatttacggc aatacaactg gcagagcaaa
      661 gagtttattc gagttggtgg cggaatttcc atatacgcgt tgcagcaatg gctgaaagcg
      721 agaggatttt ctttgcctgt tttcaccgat gtattggggc cgtctgtcgg cggttatatc
      781 tccgcctcgg gtattagttt agattcacaa aaatacggcg gtttctggaa taatgtaaat
      841 cgtgtgcgtt tagtcgatgc aaaaggtgaa atgtacgttc ttacacccaa agataaagat
      901 ttcaaatggc tatttggttc tatgggacaa ttaggagtga ttgttgaggc cgacttaaaa
      961 attgtaccgt ataagaaacc acgatttagg caagagattg gcatcatcgc agaaagtctc
     1021 gtagcaggcc ctaaaggtca gccgagagat accgaatatg aatcttctca tttagtttgg
     1081 tttacagtaa ttgcacctat cgaaaaaaaa tcagaattaa tgacggattt actaaatatt
     1141 aaagcaaaat attcggatgc aataaattac atacccgagt atatatggcc ggtgcgcaaa
     1201 ttaaatttta caccgccact tttttatccc gagaatcgag agcttatttg tataggtatt
     1261 tggggcatgc caaaatcaga taatttaaat aataataaat tcctttttgg tatcagtgaa
     1321 gatatttcaa atttaataac tcaaaaaaaa tactatcgtc gctatataca atctgaagtt
     1381 tataaaacaa aaattgattg gcgcgagtat tggggagaaa aaatttattc tgaatttttg
     1441 caaataacta aattatataa ccccaaagga aacttaaatc aaaactgtat ctttggttgc
     1501 gacagtacat atatagagcc aagtacaatg ccttaactat aaaattggct cagataaaaa
     1561 agtaagtaga tattcaatac tgccaataaa gcattggtgc ggtggcgcag gcgtatttta
     1621 taaccaataa agaaaaatag tattgttata caaagaaggc ttacagaaag aattattcgt
     1681 tgctgtatga ataaataaat ggtcggcacg agtaatccca aagtagcgat gcccataaga
     1741 aagtgatgcg cttttcttaa cccccatttt tttttgtaaa accaaatata gaatgaatta
     1801 ttttttgtct tgtaatctgt acgaacatct tttatgtctt tgaaaagtga aaagaaagac
     1861 caaccgcaaa aaataatcga aaggactacc agggtctcgt ttgatattcg tgaagttata
     1921 ggcgcaagtg taatcaatcc ccccaaatag cttaacgcac cccacaaacc ttcaaatttc
     1981 attgtggcga aaagacgacc cttagcgcga tagaatggga aactatatag aataccacaa
     2041 acgagaatca gggaggcaaa gagtgcgtac attttaccag cgacagccaa aaggattaaa
     2101 gtaaaaacag taattgctgt aaaaaagaaa acatcgccag catcaaagtg tataatccgt
     2161 ttttggagcc gatcttcttt ctgatcaaaa aaatcatttt gtacaatcat tgaaaagaaa
     2221 agtaaaaagt gcagtatcaa gaagagaatt atggccgtat tgctttgatt tgccgtgagc
     2281 atgccgtatg ctagcaacag cataaaaggc gcaatatgca agaggcgctt tagaatatac
     2341 tttatcgaaa tgcgccgcaa gagcatataa gtaaaaacag cggcaagaat ttgtaaaagg
     2401 ggtaagacta atcgtaactc aaggttatga aactccgtct tctttgtggt ataccaagtc
     2461 tgcaagagtg aaggcaatat atacgagtga aagaagaaaa gtagataaac ggcaattaac
     2521 ataaaaattg ttctaagaag atttgcacct tgtagatagg catataaagc tgcgaaggca
     2581 atcgtcaacc aaacggtgat ggcttcgccc atgggcacgg attgttcggg cataaaaaat
     2641 acgaagaagc ctgacggcaa aggaatatcg gtaaaaacat gtaaataata agcataccga
     2701 aacggcgtat cgggtctacc tgaaataatt atatctagta tgggcggtaa aagccctaaa
     2761 aatagcgcta ccaaagggac gcggtatacg cgtgattcgc ttaaaccgcc cacatatttt
     2821 aagaggatta ggatacctaa aaacatggca atataaaaag gtatataagg aagcatcatc
     2881 aaagaattaa acacttgcgg ttcgaagaat attgcttcaa gaataccgcg tatagctccc
     2941 gtaaaaaagc agaatagcaa aagggtgggg gtattgatgg ttttacccga cgagagcata
     3001 acgattcgac gtaatgaaaa gtgactcatg tttggattaa atatgtacaa attgatattg
     3061 ccatgacaag cacttaatcg tgcatgccgg tgagatattc aaaattttat ttttacaaga
     3121 ttcgtctatg aaattaaatt ctattttagc cgctgttatc tgcttgatta ctctttcttg
     3181 tcgagataaa gatttaggag tcaagaccgc gcaaaagtat tgcacaaact gtcatgcatt
     3241 gccattgcct gccgacttag ataaagaatc ctgggggctc gttctcgata gcatgggtac
     3301 gtttatcggc atcccttctc aaaataaaag tcgaccatta tttcgagccg cagcaaatac
     3361 agtcatacct aaaagaacaa ttaccgatag cgaatggcaa gcattgtccg attattttat
     3421 ctccggcgcc catcaagatg agcagccccc caaagaaaat gctaaaatgt gcaatgtttc
     3481 gtttcaaaaa acagagattc gaatcgatag ccccgtgcaa gcgattacat gggtagaact
     3541 cgatgcggca tgcaactgcc ttagatttgc agaaggtatt caaggtgatt tgtttgaagc
     3601 cgatttaaaa ggtaaaattc gtaaaaagat acatgttggt tattctgtaa ccggctcggt
     3661 agaaattcat ggtaaaaaat attttgtcgc tgcaaaaaat cttttaccta acgatgaccc
     3721 cagcggtatt ctattcacgc tgaatggtca aacgccaaaa atcttgcgta atgatttaat
     3781 tcgacccgta tacataacga gttttacaga taacaataaa gtaccattct taattatatc
     3841 tgaatttggg cacacccaag gtagattgag taagataaat ctgactaatt atgaacgcac
     3901 aactttatcc gatttatcgg ggtatataag cagcttgata aatggtaaag gtgaactgct
     3961 tgcgctccgt gcacaggccg acgaagcgat tgtacaattc gcgccgaata taaaaaatct
     4021 gattcgtttt tcaccgtcat ggggctcgac ttcgatagca tggacgacct ttgatgcaca
     4081 aaataaagaa tatcttgtta caagccatgg cgacaatggt gatttacccg atatgccgta
     4141 taagcctgca cacggtattc gcgtttacga gaatatcgat aataactggg cgcaaaaata
     4201 tttttatccg atgcctggcg cctacaaagt gatgactggc aagttttttg aaaaacaaaa
     4261 aacagactga cggtgtgtgg gtggcacatg atgtattgtg agttgtggag tgggtgggca
     4321 tgcttagtgt gtgtatgtgt gttgtggagt tgcttagtga gttgtgtgat gcgatgcgtg
     4381 aggtggtgac ggagttagac cagcaccaac acac
//
LOCUS       DAOMPD010000199         4411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8248_length_4411_cov_3.068182, whole genome shotgun sequence.
ACCESSION   DAOMPD010000199 DAOMPD010000000
VERSION     DAOMPD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8248_length_4411_cov_3.068182"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLY93_07870"
     CDS             <1..416
                     /locus_tag="PLY93_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HRP69432.1"
                     /translation="GEYSKAGEFVYDHPFLWGSKRTGPDLHRVGGKYNHAWHYQHMLD
                     PRKTSPGSIMPAYPWLFDHSLDTSLTAKKVKALQSIGVDYPVGYDKQALADLQNQSKK
                     IADDLTQNGMKVTADKEIISVIAYLQRLGTDIKAK"
     gene            423..614
                     /locus_tag="PLY93_07875"
     CDS             423..614
                     /locus_tag="PLY93_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69433.1"
                     /translation="MIGKVLNDLNGVAIYPIVTLLLFFSVFGFTLIKVLKRKKSDDIE
                     MANLPLEDGVTGKREANHG"
     gene            607..1182
                     /locus_tag="PLY93_07880"
     CDS             607..1182
                     /locus_tag="PLY93_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRP69434.1"
                     /translation="MDKRTEEEKLMEHEYDGIRELDNHMPRWYVLALYASFIFCVGYL
                     IYYHVMGGPTQEQEYEMEMAAAPKMVKPAAVATDAAAKPLTDAASLKAGQDLFKSHTC
                     FACHKDDMGGMVGPNLTDEFWMHGCDYATVITNVSTGFPQKGMPPFGNGTPMNETQLQ
                     QIVSYIFSKRGTNPAGAKAIDKVREVKCVAK"
     gene            1243..2742
                     /gene="ccoG"
                     /locus_tag="PLY93_07885"
     CDS             1243..2742
                     /gene="ccoG"
                     /locus_tag="PLY93_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRP69435.1"
                     /translation="MSLSTDPLAKIEADSEAFRDHIGTITAQGKRAWVYAKKPKGRFY
                     RWRSLVGYLLLFLMFAGPYVRLHGQPLLLLNVLENKYIIFGFVFWPQDFYLAVLGILS
                     LVLTITLFTSVFGRIWCGWTCPQTVFLEMLFRKIEYAIEGDFQQQKALAASPWNANKI
                     LRKGIKIAVFYALSFAIANTFLAYIIGSEALWQIIREPISQHVVGFLTINAFSIVFFL
                     VFARFREQVCQFACPYGRLQSALVDNDTISVTYDFVRGENRGTLGTRKKAAKAMQTDI
                     VSGANLGASSSFGDCIDCGACVVVCPAAIDIRNGIQLECIQCTACIDACDKMMDAVGK
                     PRGLIRYTSYNNVLASKDKTHKPQRRFPIKLRIVGYMALWFLLVSVFSYLILARENIE
                     TVLLRQPGMLAHEMPDGAVGNLYTLKFFNKTFDDQNVELKLANGEGEIRLIDPLDKIE
                     AQKVVEVRLFIALKKENIKGGSMPLSIEVWSNGKKVNTRSTTFISTGGI"
     gene            2742..3212
                     /locus_tag="PLY93_07890"
     CDS             2742..3212
                     /locus_tag="PLY93_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRP69436.1"
                     /translation="MEKIKELTFWDKNAWPIGLVVALLLFFSMTGYFVSVAFGERVDL
                     VADDYYYRDKEFSARLEREKKLIALGGGSITRAHDGVSIELPTFFQNKKSTGTLFVYS
                     PLNPADDFSVPVSFEGRNATFKLPLDVKHRWRLSLDFTANEKGYYLTSTLNASL"
     gene            3209..3877
                     /locus_tag="PLY93_07895"
     CDS             3209..3877
                     /locus_tag="PLY93_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRP69437.1"
                     /translation="MSFYLSALMLGLFSQGHCLGMCGPLVLALPVNEKSRALSIFYRM
                     LYNVGRITTYCVLGFLVGLLGVLVSLAGVQAYISYIVGFTLIFVALLQLMPFLRVHRF
                     SSLHTRISQSLRSFWGRAGAFKFFWMGAVNGLLPCGMVSSALIASLAAASALQSMLFM
                     FWFGLGTAPMMLAASLFGVYLSPRTRKVLAVMGPLFSIALGVYLLLRPSLFGTHVHTH
                     GHHH"
     gene            4030..>4411
                     /locus_tag="PLY93_07900"
     CDS             4030..>4411
                     /locus_tag="PLY93_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRP69438.1"
                     /translation="MSLSNKNNLHFKGALTFPGDKSLSHRAALLAALAEGTSTLVNFL
                     DASDTMNTLKAVEALGVKVTALGNQSFRVESPGARHLTSPATTIDCGNSGTGSRLLLG
                     MLAGLDGVTAIIDGDASLRTPPMRR"
BASE COUNT         1063 a         1052 c         1114 g         1182 t
ORIGIN      
        1 acggcgagta ctctaaagcg ggcgagtttg tgtacgacca tcctttcttg tggggctcta
       61 aacgaacagg gccagacctc caccgtgtgg ggggcaaata taaccacgca tggcactacc
      121 aacacatgtt agacccgcgc aaaacatcgc ctgggtcaat catgcctgcg tacccttggc
      181 tttttgacca ctcgttggac acttcgctca ccgccaaaaa agtaaaagcg ttgcagtcga
      241 ttggtgtcga ttatcccgta ggctacgaca aacaagcgct cgccgattta cagaatcaat
      301 cgaaaaaaat cgccgatgac ctcacccaaa atggtatgaa ggtcaccgcc gataaagaaa
      361 ttatttctgt gattgcatac ttgcaacgcc tgggaaccga cattaaagca aagtaggcgg
      421 cgatgatcgg taaggtatta aacgatttga acggcgtcgc tatctacccg atagtgacgt
      481 tgctcttgtt tttctcagtg tttggtttta cactgataaa agttttgaaa cgaaaaaaat
      541 ccgatgacat cgagatggca aacttgccgc tcgaagacgg agtaacaggt aagcgggagg
      601 cgaaccatgg ataaaagaac cgaagaagaa aaattgatgg agcacgaata cgacggtatt
      661 cgagagctcg acaaccacat gccgcgttgg tatgtgttgg cgctttatgc aagttttata
      721 ttttgtgtgg gctatttaat ttactaccac gttatgggcg gcccgacgca agagcaagaa
      781 tacgaaatgg aaatggcggc tgcaccgaaa atggtgaagc ctgctgcggt ggcaaccgac
      841 gccgctgcaa agccgctcac cgacgccgct tcgctgaaag cgggacaaga ccttttcaaa
      901 tcgcacactt gctttgcgtg ccacaaagac gatatgggcg gtatggttgg tcccaacctc
      961 accgatgaat tttggatgca cggctgcgat tatgcaacgg tgattacgaa tgtatcgacg
     1021 ggatttccgc aaaaagggat gccccctttt gggaatggta cgcccatgaa tgaaactcaa
     1081 ctgcaacaaa ttgtgagtta tattttttca aaacgaggta caaacccagc gggcgcgaag
     1141 gctatcgata aagtacgcga agtgaagtgc gtggcgaaat aagtccgcat ggattgcaaa
     1201 ttttatcgta taaaaagttt tgagtaagga gcaaaagaca acatgagttt atcgaccgac
     1261 ccattggcca agattgaagc cgacagcgaa gcgttccgcg atcacatcgg cacgattacc
     1321 gcacagggta aacgcgcatg ggtgtacgca aagaagccca aagggcgttt ctatcgatgg
     1381 cgttcgttgg tgggttatct cttgttgttt ttgatgtttg ccgggcctta cgtgcgcctt
     1441 cacggccaac cgcttttact cctcaacgtc ctcgaaaata aatatattat ttttggattt
     1501 gttttctggc cgcaagattt ttatttggcg gtgttgggca ttctttcttt agtcctcacg
     1561 attactctct tcacgtcggt cttcggtcga atatggtgtg gatggacttg cccgcaaacc
     1621 gtgttcctcg aaatgctttt ccgtaaaatt gagtacgcca tcgaaggcga tttccaacag
     1681 caaaaagcgc tcgctgcttc accgtggaac gcgaataaaa ttttgcgcaa aggtataaaa
     1741 attgccgtct tttatgctct ctcgtttgcg attgcaaaca ccttcttggc gtacatcatt
     1801 ggctcggaag cgttgtggca aattatccgc gaacccatct ctcaacacgt cgtcggattt
     1861 cttacaatca acgctttttc aatcgtgttc tttttagtct tcgcgcgttt ccgtgagcaa
     1921 gtgtgccagt ttgcatgccc ttatggacgc ttacaatcgg cgctcgtgga caacgacacg
     1981 attagcgtca cgtacgactt cgtgcgcggt gaaaaccgtg gcacgctggg cacacgtaaa
     2041 aaagcagcga aagcgatgca gacggacatc gtgtccggtg cgaaccttgg agcatccagc
     2101 tcctttgggg attgcatcga ttgcggtgcg tgtgttgttg tatgtcctgc ggcgattgat
     2161 attcgtaatg gcatccagct ggagtgtatc cagtgcactg cttgcatcga tgcgtgcgat
     2221 aaaatgatgg atgctgtagg gaaaccgcgt ggtttaatcc gctatacatc atacaacaac
     2281 gtccttgcga gtaaagacaa aacccataaa ccccagcgta ggtttcccat caaattacgt
     2341 attgtgggct acatggctct ttggtttctt ttggtttctg ttttctcata tcttatcttg
     2401 gcgcgtgaaa atattgaaac cgttctctta cgccaacccg gaatgctcgc gcacgaaatg
     2461 cccgatggtg cggtgggcaa tctttatacg ctcaaatttt ttaataagac atttgatgat
     2521 caaaacgtcg aactcaaatt ggcaaatgga gaaggcgaaa tccggcttat cgatccctta
     2581 gataaaatcg aagcgcaaaa agttgtcgaa gtgcggctct ttattgccct taaaaaagaa
     2641 aatataaaag ggggaagtat gccgctgtcg atcgaggtgt ggtcgaatgg aaaaaaagtc
     2701 aacacccgct cgacgacgtt catcagcacg ggcggtatct aatggaaaaa ataaaagagc
     2761 taaccttctg ggataaaaac gcatggccga ttggcctcgt cgttgcactg ctgctctttt
     2821 tttcgatgac gggttatttt gtttctgtcg cgtttggaga gcgcgttgac ttagtcgccg
     2881 atgattatta ttaccgtgat aaagaatttt cggcacgctt agaaagagaa aaaaaactca
     2941 tcgcgttggg aggcggcagc atcacccgag cgcacgacgg tgtgtcgatt gagctaccga
     3001 ctttttttca aaataaaaaa agtacaggca cgctttttgt ctactcgccg ctcaaccccg
     3061 ccgacgattt ttctgtgccg gtgtcttttg aggggagaaa cgcaacgttc aaacttccgc
     3121 tcgatgtaaa acaccgttgg cggctctcat tggattttac tgcaaacgaa aagggatact
     3181 acctcacctc gactctcaac gcgtcgctat gagtttttat ctctcggcgt tgatgctggg
     3241 tctcttttca caagggcatt gcttgggtat gtgcggcccg ttggtgttgg cgctgccagt
     3301 caacgagaag agccgcgcgc tctctatttt ttaccgcatg ctctataacg tgggacgcat
     3361 caccacgtac tgcgtcttgg gttttcttgt cggtctttta ggtgtgttgg tttcgctcgc
     3421 gggcgtgcaa gcgtatattt cctatatagt gggattcaca ttgatatttg ttgcactttt
     3481 acagttgatg ccattcttgc gcgtgcatcg tttttcttca ctgcatacac gcatttcaca
     3541 atcgctcagg agtttttggg ggcgcgccgg tgcgttcaaa tttttttgga tgggcgcagt
     3601 caacggtctt ttgccgtgcg gtatggtgag ctctgcgctc attgccagcc ttgctgccgc
     3661 gagtgcgctc caaagtatgc tgtttatgtt ttggtttggc ttaggtaccg cgcccatgat
     3721 gcttgccgca tcgctttttg gtgtgtactt gagcccgcgc acacgcaagg ttttggcggt
     3781 gatgggaccg cttttttcaa tcgcgttggg tgtgtatctt ttgctgcggc cttcgctttt
     3841 tggaacgcac gtacatacac atgggcacca ccattgacat cccgctaaat tgcagtcttt
     3901 cataaacatc ttagagtctg tgcgccaact cgcgtagtgt gtacaaaaga attgtctcag
     3961 cgagttggcg cacaggcccc ttataagtcc tttacgggca ctttctgtta ttgtgtcagt
     4021 cacgcacctg tgtcattgtc aaataagaat aatcttcatt ttaaaggtgc actcactttt
     4081 ccgggggata aatcgctgtc gcaccgcgcc gcacttttag cagcgctcgc cgagggcaca
     4141 tcgacgctgg tgaatttttt agacgcaagc gacaccatga acacgctcaa ggcggtcgaa
     4201 gcgttgggcg taaaagtgac ggcattggga aaccaatctt tccgcgttga atcccctggc
     4261 gcgcgccacc tcacttctcc cgcaacgacc atcgattgcg gcaactcggg caccggttcg
     4321 cggcttttgt taggaatgct tgcggggctc gacggcgtca ctgcgattat cgacggcgac
     4381 gccagtttac gcaccccccc catgcggcgc g
//
LOCUS       DAOMPD010000200         4405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8265_length_4405_cov_2.604598, whole genome shotgun sequence.
ACCESSION   DAOMPD010000200 DAOMPD010000000
VERSION     DAOMPD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8265_length_4405_cov_2.604598"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..>1359
                     /locus_tag="PLY93_07905"
     CDS             295..>1359
                     /locus_tag="PLY93_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRP69439.1"
                     /translation="MRFGLQFKLTALIVALIASVIGSLAFYIAREKKNILTETASATA
                     RKELASLAFIASRAILDRDDLALADSLQNAQGLLGFAVGAVYTPSKKTPLISRVGKSL
                     TDKQKEIESDALAVAREEWDKLIPLHPGYTAKNRKEGDTNQNKKNIEKKENPKTTKNV
                     QPRKGESPKHSSVATAPELLRITQRDFLGNKNIKIQYFHKAVFHPFIKENPPLLAVAQ
                     VAVTDEYINKSIRESFIELAIVGALFFLAALIAAYLLSRFIVKPVKVLSHGADEVGGG
                     NLDVTLPPLGSDELGQLAKQFNAMTASLKRASAEAKEQAVFNDQVRQAQEIQEGMNPQ
                     RFLKKPEYQIKGFTRAAKGVG"
     assembly_gap    1360..1369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1370..1980
                     /locus_tag="PLY93_07910"
     CDS             <1370..1980
                     /locus_tag="PLY93_07910"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRP69440.1"
                     /translation="YYDFQLMGDGRLAVLISDVSGKSISASLVMVLIKTVVSTYLKLF
                     NTIRGDTIVRTINRVMCGEAHIDKFATILFYIFDPVAKTIEFTNGGHGPIFIYRADRR
                     VCTSTKLEGLPMGIEEDNVYRLAKLSVNPGDIVLLYTDGITECWNQNKEEFGLKRVRE
                     KIIEYADKNPKEIIEHLVKDLDAFADGAEQHDDMTMVVMKVL"
     gene            1996..2721
                     /gene="cmoA"
                     /locus_tag="PLY93_07915"
     CDS             1996..2721
                     /gene="cmoA"
                     /locus_tag="PLY93_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HRP69441.1"
                     /translation="MKDEIFNAPGVGKTPFEFNSRVVAVFDDMVSRSVPFYRDVLAMT
                     AELAREFYQPQTKIYDLGCSTGALLDAIVKKFENTRFIYHGVDNSPEMLKKAREKYSA
                     LNECHHLEWQCADIVQVDFEPASVFVSNYTFQFLKPLMRKTLLQKIFNALTQGGALLL
                     SEKCLEDSADVSRIFADRYHAMKEANGYSKLEIAEKRDALENVLIPFRVSENIDMLRE
                     AGFTPVSIFFKAYNFVSFLALKA"
     gene            2725..3627
                     /gene="cmoB"
                     /locus_tag="PLY93_07920"
     CDS             2725..3627
                     /gene="cmoB"
                     /locus_tag="PLY93_07920"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011650.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HRP69442.1"
                     /translation="MTLTEIEESIKKINFTPDTVSFLRSDGKPQLQVDNQSNGRVLCS
                     GNYNDATSPGLAAMLAEALIPWRKGPFQIDGFFIDAEWRSDFKWNRLLPDLPNLSGKT
                     VADVGCNNGYYLFRIAEAGAVRTVGFDPTLKYKLQYELIAAHAPERGIEYHLAGWQDL
                     AAWPCAFDVIFLMGVNYHASDTTEVLHACKKALKEGGLLVLESVVVPTDALLEIFPHG
                     KYAGIGGVYAIPSPAALADDLANVGFKNIFEQHVVRMTREEQRSTLFSPQKSLEDFLN
                     EDGSSIEGYPPLYRAAFFATKNLE"
     gene            complement(3640..>4405)
                     /locus_tag="PLY93_07925"
     CDS             complement(3640..>4405)
                     /locus_tag="PLY93_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HRP69443.1"
                     /translation="KVRCFPIEKVAKKICLAQARIKAWRMSEALFQKRWPVKYPDRLQ
                     LYSYPTPNGVKISIMLEEIGLPYEAHRIDILKDETWTTEYLSLNPNGKSPAIDDPQGP
                     DGKPFVLFESGAILLYLAEKTKKLLPHDAAKRYETIQWVFFQMAAVGPIFGQVGFFHK
                     FAGKEFEDKRPLMRYVNESKRLLGVMEKHLTQKTWFMGDEFTIADIAHLGWVKNLIGF
                     YEAGELVEYNKLKAVPAWLERGLNRPAVARGLTIPS"
BASE COUNT         1163 a          993 c         1082 g         1157 t
ORIGIN      
        1 gaacaagggt tgttagtggg gagcggaaat tttccgatgc tggcaaaaaa tggaaacgaa
       61 cctgtgcgtc gtttcttttc aggccccgca cttttcaggg tgcgcgataa atcagatggg
      121 agcaataatc ttttctaatg cattctgctg ggtattaatg tcaaagacct cgcaatttgc
      181 aaaaaaagtt acctcagcga gttggcgcac agactcctcc aataccccca ttagatttgt
      241 cgccactcta cttttcactg ttcaaccata aaagacttta gagtttgtac ttccgtgcgc
      301 ttcggcttac aattcaaact caccgcgctt atcgttgcac tcattgcctc cgtcattggt
      361 tcgcttgcat tttatatcgc acgcgagaaa aaaaatatac ttactgagac agcctctgcc
      421 actgcgcgca aagaactggc ttcgcttgcg tttatcgcgt cgcgcgcaat tttagaccgt
      481 gacgatttag ctcttgccga ttcgctccaa aatgcgcaag ggcttttggg ctttgctgtc
      541 ggtgcggttt ataccccttc aaaaaaaaca ccgctcatca gccgcgtggg taaaagcctc
      601 acggataaac aaaaagaaat cgagagcgat gcgttagccg ttgcgcgcga agagtgggat
      661 aaactcattc cattgcaccc gggctatact gcaaagaacc gtaaagaagg cgatacaaat
      721 caaaacaaaa aaaatataga aaaaaaagaa aatcccaaaa cgacaaaaaa tgtgcagcct
      781 cgaaagggtg aatctccaaa gcactcatcg gtggcgactg cgcccgaact tctacgcatc
      841 actcaaagag attttttggg aaataaaaat ataaagattc agtattttca taaagcggtg
      901 tttcatccgt tcatcaaaga gaatccgcca cttttagccg tggcacaagt ggcggtgact
      961 gacgaatata tcaacaaatc gattcgtgaa agttttatcg aactggcgat tgtgggcgcc
     1021 ctttttttct tagcggcatt gattgcagcg tacttgctct cgcgctttat tgtgaagccg
     1081 gtaaaggtgt tgtcgcacgg cgccgatgaa gtggggggcg gtaacctcga cgtgacactg
     1141 ccgcctttgg gctcggatga attgggacaa ctggcaaaac aattcaacgc gatgacggcg
     1201 agtcttaaac gcgccagcgc ggaggccaaa gagcaggcgg ttttcaacga tcaagtgcgg
     1261 caggcgcaag aaatccaaga aggaatgaat ccgcagcgtt ttttgaaaaa gcctgaatac
     1321 caaatcaaag gattcacgcg tgcggccaaa ggcgtcggcn nnnnnnnnna ctattacgat
     1381 tttcaactca tgggagacgg gcgacttgcg gtgcttattt cagacgtgag tgggaaatcg
     1441 atttcagcgt cgctcgtgat ggtgctcatt aaaaccgtcg tctcgacata cctcaaactt
     1501 ttcaatacca tccgtggcga caccatcgtg cgtaccatca accgcgtaat gtgcggcgaa
     1561 gcgcacatcg ataagtttgc aaccatcttg ttttatattt ttgatcccgt cgcaaagacg
     1621 attgaattta cgaacggcgg ccacgggccc atttttatct accgcgccga tcgccgcgtg
     1681 tgtacgtcta ccaaattaga aggcttgccg atgggtatcg aagaagacaa tgtataccgt
     1741 ttggcgaaac tttcggtaaa ccctggcgac attgtgcttc tttacactga cggcatcacc
     1801 gagtgctgga atcagaacaa agaagaattt ggattgaaac gcgtgcgtga gaaaattatc
     1861 gaatacgcgg ataaaaatcc caaagaaatc atcgaacact tggtgaaaga tttagacgcg
     1921 tttgccgacg gagcggaaca gcacgacgac atgacgatgg tggtgatgaa agttttgtag
     1981 tgtgatgtat tctctatgaa agatgaaatt tttaacgccc ctggtgtcgg taaaacgccg
     2041 tttgaattta actcacgcgt cgtcgccgtt tttgacgaca tggtttctcg ctcggtgcca
     2101 ttttaccgcg atgttcttgc gatgacggcg gaactggctc gcgaatttta tcaaccacaa
     2161 acgaaaattt atgatttggg atgttccacc ggtgcattac tcgatgcgat tgtaaaaaaa
     2221 ttcgagaaca ctcggtttat ctaccacggt gttgataact cgccggaaat gcttaaaaaa
     2281 gcgagagaga aatacagcgc cctcaacgag tgtcatcatt tggaatggca gtgtgccgat
     2341 atcgtccaag tagattttga accggcgtcg gtttttgtct caaactacac gtttcaattc
     2401 ttaaagcctc ttatgcgcaa gactcttttg caaaaaattt ttaatgcact cacacaaggc
     2461 ggcgcgcttc ttctttctga aaaatgcctg gaggactcgg cagatgtttc gcgtattttt
     2521 gccgatcgtt accatgcgat gaaagaagcc aacgggtata gtaaacttga aatcgctgaa
     2581 aagcgcgacg cgctcgaaaa tgtgttaatc ccttttcgtg tgagcgaaaa catcgacatg
     2641 ctgcgcgaag cgggatttac tccggtgtcg atttttttca aagcgtacaa ttttgtttcg
     2701 ttcttggcgc tgaaggcgta acgcatgact ctgactgaaa ttgaagaaag tataaagaag
     2761 ataaatttca ctccagacac tgtttctttt ctgcgtagcg atggaaaacc acagctacag
     2821 gtggataacc aaagcaatgg ccgtgttttg tgtagcggga attacaacga cgccacttcg
     2881 ccgggtcttg cagcaatgct cgcggaagcg ttaatcccgt ggcggaaggg cccgttccaa
     2941 atcgatggat ttttcatcga cgcagagtgg cgcagcgatt tcaagtggaa tcgccttctg
     3001 cccgatttac cgaatttgtc gggaaagacg gtcgccgacg tgggttgcaa caacggatac
     3061 tatctctttc gtatcgccga agcgggggca gtgcgcacgg ttggttttga tcccacgcta
     3121 aaatacaaac tgcagtacga attgattgca gcgcatgcgc ccgagcgcgg tattgagtac
     3181 catttggcgg gctggcaaga cctcgcggca tggccatgcg ccttcgatgt tatttttcta
     3241 atgggggtta actaccacgc gagtgacact accgaagttt tacacgcgtg caaaaaggcg
     3301 ctcaaagaag gtgggctttt ggttttagaa tcggttgtcg tccctaccga cgcattgttg
     3361 gagatttttc cacacgggaa atatgcgggc atcggtgggg tctatgccat cccttcgccc
     3421 gctgcgctcg cggacgacct tgcgaatgtg gggttcaaaa atatttttga gcaacacgtt
     3481 gtacgtatga cgcgtgagga acaacgatca acactgtttt ctccacaaaa aagtttagag
     3541 gattttctca acgaagacgg cagcagcatt gagggatatc ctcccttgta cagagcggca
     3601 ttttttgcta caaaaaactt ggagtgaatt gtacctaaat catgatggta tcgtgagccc
     3661 acgtgcgacc gctggacgat tgagaccacg ttcgagccat gcaggtacgg ctttaagttt
     3721 attgtactcg acgagctctc cggcttcata aaaaccaata agatttttca cccaacctaa
     3781 atgtgcaata tcggcgatgg tgaattcgtc gcccatgaac caagtttttt gagtgaggtg
     3841 tttttccatc actcccaaaa ggcgtttgga ttcattcaca tagcgcatga gagggcgctt
     3901 atcttcaaat tccttgccgg cgaatttatg aaaaaatccc acttgaccaa atatcgggcc
     3961 cactgccgcc atttgaaaaa atacccactg gatggtttca taacgctttg ctgcatcgtg
     4021 cggcaaaagt ttttttgttt tttcggctaa gtaaagaaga atcgcgccag attcaaaaag
     4081 tacgaagggt tttccatcgg ggccttgtgg gtcgtcaatg gcgggacttt ttccattggg
     4141 attgagagac agatattctg ttgtccatgt ctcgtctttg aggatgtcga tgcggtgggc
     4201 ttcgtagggt agtccaattt cttcgagcat aatcgatatt ttgacgccgt tgggtgtggg
     4261 atacgaatag agttgaaggc ggtcagggta ttttactggc catcgttttt ggaaaagtgc
     4321 ttcgctcatg cgccatgctt taattcgcgc ctgggcgaga cagatttttt ttgcaacttt
     4381 ttcaatagga aaacatcgca ctttt
//
LOCUS       DAOMPD010000201         4397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8286_length_4397_cov_2.455090, whole genome shotgun sequence.
ACCESSION   DAOMPD010000201 DAOMPD010000000
VERSION     DAOMPD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8286_length_4397_cov_2.455090"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1488)
                     /locus_tag="PLY93_07930"
     CDS             complement(112..1488)
                     /locus_tag="PLY93_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRP69444.1"
                     /translation="MATPRKIVCLGAGYVGGPTMVVMASHNLDMEFYVTDQNAARINA
                     WNSDTLPVFEPGLDEVVKRVRGKNLFFKVIEPSLLASADIVFVCVGTPTKEYGEGKGM
                     AADLQFTELAVRDIEKYCKSGTIIVEKSTVPVRTAEAILNIVNTQEKGKKFEVLSNPE
                     FLAEGTAIQDLQNPDRVLIGHADTDSALSAAEIIKTLYTAWVKPEKVLLTNVWSSELS
                     KLVANAFLAQRVSSINSISALCEKTNASVKQISRAIGADSRLGSRFLEASVGFGGSCF
                     KKDILNLVYICRREGLLEVADYWQAVIDMNDYQTRRFVSQVIETQFSSVSGKKIAVLG
                     FAFKADTNDTRESPAIYVCKRLIEEKAQLFIHDPQALASAKHDLRGIDNSVVYTTDIE
                     EATRGAHALVILTPWAEYKNLNYAKVFESMVKPAFVFDGRSVVDAQKLHHIGYNVKQI
                     GISPLMHF"
     gene            complement(1494..>2815)
                     /gene="gatA"
                     /locus_tag="PLY93_07935"
     CDS             complement(1494..>2815)
                     /gene="gatA"
                     /locus_tag="PLY93_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRP69445.1"
                     /translation="AFLAVEKEEALAAAKAAEKRIQAGRALSPYDGIPIGIKDNIVTK
                     GRTTQCASKILDGFISPYSATVIEKLQALGFVTVGRLNMDEFAMGSSTENSAFQTTKN
                     PFDTTRAPGGSSGGSAAAVAAGYLPVSLGSDTGGSIRQPAAFCGLAGLKPTYGTVSRY
                     GLVAFASSLDQIGPLAHTVSDVEIIHNAIKGHDTKDSTSVPDPAYVSWNGELNDIRIG
                     VDEAMLDGISPEVRASFDKTLQFLKTKVKSVTSVTLPNQKYGIPVYYLVATSEASANL
                     ARFDGVRFGHRAGDAKNLNELYEKSRTEGFGAEVKRRILLGTYALSSGYYDAFYGKAQ
                     AVRALIQKDYQTAFHGVDAILMPATPSPAFKLGEKTHDPVQMYLSDVLTVGASLAGIP
                     ALSVPGPTDGLPIGMQLQGPNFSEAMLFALGKAIEAEFPQARASLAK"
     assembly_gap    2816..2825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3044..>4397
                     /gene="lpdA"
                     /locus_tag="PLY93_07940"
     CDS             3044..>4397
                     /gene="lpdA"
                     /locus_tag="PLY93_07940"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRP69446.1"
                     /translation="MNFDYIVIGAGPGGYSSAIRAAQLGLKTAVVERENAGGVCLNWG
                     CIPTKALLTSAHLKHDAGKLGIQVSGSVQADLPSIIDRSRQVAARLQTGVKSLFKKYG
                     VTLIEGSATFTAKNTIKVKNPTGETTLNFKNACIATGARARALPQLPFSQRVWQYRDA
                     LTVKKLPTSLIVVGGGAIGLEFADFYRCLGSEVTVIEMAPHILPNDDVKVASHLRKAL
                     EQRGMKFLEGAAITTLKENANGVSLTLKDSAGKDTTLDAAVLLVAIGVQPNTEELGID
                     AWGICNDKKFITVDENFCTQEKNIFAIGDVTQGKLLAHRAAHQGIYVAEFLAGKNPRQ
                     IPAIPSCIYTSPQVAAVGMNEKELKEIKREYEMGEFPWLASGMAIAAGEQNAFTRVFT
                     DKKTGEILGAQVVGENAAELIGQVSLAMASELLAEDFLHAVFPHPTLAETIPEAFGVL
                     M"
BASE COUNT         1073 a         1076 c         1127 g         1111 t
ORIGIN      
        1 cgaaaaggag ggtaaatttt gaggaaaaac tgtccttcag acgagcaggc caccgattct
       61 tcgcgccaat gcgactataa tacatccttt gcagcgagtt agatactttt ctcaaaaatg
      121 catcagcggg ctaatgccga tttgttttac gttgtaaccg atgtggtgta gcttttgtgc
      181 atctaccaca ctgcgcccgt caaagacaaa cgcgggtttg accatacttt caaaaacttt
      241 ggcgtagttt aaatttttat attctgccca cggggttaaa atgaccagcg cgtgtgcacc
      301 gcgtgtggct tcttcgatgt cggtggtata aacgacagag ttgtcaatcc ctcttaaatc
      361 gtgtttggcg cttgccaacg cttgcgggtc gtggataaaa agttgcgctt tttcttcgat
      421 gagccgctta cacacataga tagcgggcga ctcacgcgtg tcgttggtat cggccttgaa
      481 ggcaaagccg agcaccgcaa tttttttacc cgagacgctg ctaaactgtg tctcgatcac
      541 ttgcgataca aagcggcggg tttggtaatc gttcatatcg atgactgcct gccaatagtc
      601 cgccacttca aggagtcctt ccctgcgaca gatataaacc aaattcaaaa tatctttttt
      661 gaagcacgag ccaccgaagc ccacgctcgc ttccaaaaag cgcgagccta agcggctgtc
      721 agcgccaatc gctcggctga tttgtttcac tgacgcgttc gtcttttcgc acaatgctga
      781 gatgctgttt attgacgaga cacgctgcgc caaaaacgcg ttggccacga gtttggaaag
      841 ctcgctcgac cacacgtttg tcaataaaac tttttctggt tttacccacg ctgtgtagag
      901 agtcttaata atttctgctg cagaaagcgc actgtcggta tcggcatggc cgatgagcac
      961 acggtcgggg ttttgcaaat cttggatagc cgtgccctct gcaagaaatt ctgggttgga
     1021 gagcacttca aattttttgc ctttctcttg agtgttcacg atattcaaaa tggcttcggc
     1081 ggtgcgtact ggcaccgtac ttttttcaac aatgatggta ccgcttttgc aatacttttc
     1141 aatatcgcgc accgctaatt cagtgaactg taggtcggcg gccatgcctt taccttcgcc
     1201 gtattctttg gtgggagttc ccacgcacac aaagacaata tctgcgctgg ctaaaagcga
     1261 aggctcgata actttgaaaa ataaattttt gccgcgcact cgctttacaa cttcatctag
     1321 accgggttca aaaacgggga gagtgtcgct gttccacgca ttgatacgcg cggcattttg
     1381 gtcggtgacg taaaactcca tatcgagatt gtgcgatgcc atgacgacca tcgttgggcc
     1441 accgacatac cctgcgccta aacaaacaat ttttcgcggt gttgccatgg ttgctacttt
     1501 gctaaagatg cacgcgcttg ggggaattcc gcttcaatcg ctttacccaa tgcaaagagc
     1561 atcgcttctg aaaaattagg accttgcagt tgcataccga tgggaagacc atcggtaggg
     1621 ccgggtacag agagggcggg gataccagcc aaggatgcgc ctaccgtcaa gacatccgat
     1681 aagtacattt gcacagggtc gtgtgttttt tcgccaagct taaatgcggg tgagggggtt
     1741 gctggcatga ggattgcatc cacgccatgg aatgcggttt ggtaatcttt ttggatgagc
     1801 gcacgcaccg cttgcgcttt gccataaaat gcgtcgtagt aaccactcga gagcgcgtac
     1861 gtacccaaga gaatgcggcg tttgacttct gcgccaaaac cttcggtgcg tgatttttca
     1921 tagagttcgt tcaaattttt tgcatcgcct gcacgatggc caaagcgcac gccgtcgaaa
     1981 cgcgcgaggt tagccgacgc ctcactcgtc gccaccagat agtaaaccgg aattccatac
     2041 ttttgattgg ggagtgtaac cgatgtcacc gattttactt ttgttttcag aaactggagc
     2101 gtcttgtcaa aagacgcgcg cacttcaggg gatatcccat caagcatcgc ttcatccacg
     2161 ccaatgcgga tgtcgttcaa ctccccgttc cacgacacat acgccgggtc gggtacgctt
     2221 gtggaatctt ttgtatcgtg gcctttgatg gcgttatgga tgatctccac atcactcact
     2281 gtgtgcgcga gggggccaat ttggtcgagg ctcgacgcga aggcgacaag gccataccgt
     2341 gacacggtgc cgtacgtggg ctttaatccc gcaaggccac aaaatgcggc tggttgacgg
     2401 atagagccac ccgtgtccga acccaaactc acagggaggt agcccgccgc cactgccgct
     2461 gccgagccgc ccgatgaacc gccgggagcg cgtgtggtat caaacggatt tttggtggtt
     2521 tggaatgctg agttttcggt agaagaaccc atcgcgaact catccatatt gaggcggcca
     2581 acggtgacaa aacccaacgc ttgcaatttt tcaataactg tggctgagta tggagaaatg
     2641 aagccgtcta aaatttttga tgcacactgt gtggtgcgcc cttttgtaac gatgttatct
     2701 ttgattccaa tgggaatacc atcgtacggg gaaagtgcac gacctgcctg aatacgtttc
     2761 tctgctgcct ttgccgcagc aagtgcttct tctttttcga cggcaaggaa tgcttnnnnn
     2821 nnnnncgtgt ttctcaattt tggcaaatgt attgttcaag agttcaacgg gtgaaatctt
     2881 ttttgaggcc aaattacgcg aaaagtcgtc taacataagt gcttatgtag gaacagaaac
     2941 gcggcgtaaa gaacctaagc aataacttat gaaaattaag tctctttaaa tgttgctggt
     3001 tgtaacatcc ttgtcgcggc aagcctcggt acatccatgt accatgaatt ttgattacat
     3061 cgttattggc gccgggcctg gtgggtatag ttcggcgatt cgcgcggcgc agttgggact
     3121 caagactgcc gtcgtcgagc gtgagaatgc gggcggggtt tgtctcaact ggggttgcat
     3181 tcccactaaa gcattactca cctcggcgca tcttaaacac gacgccggta agctgggcat
     3241 tcaagtgagc ggtagtgttc aagcggattt accttcaatc atcgaccgct cacgccaagt
     3301 tgcggcgcgg ctccaaaccg gagtaaagtc gctgttcaaa aaatatggcg tcactctcat
     3361 cgaaggctcg gctaccttta cggcaaaaaa cacgatcaaa gtaaaaaatc ccacgggtga
     3421 aaccacattg aattttaaga acgcgtgtat cgccacggga gcgcgcgcgc gcgcgttgcc
     3481 acagttgccg ttttcccagc gtgtgtggca gtaccgcgat gcactcaccg tgaaaaaatt
     3541 acccacatcg ctcatcgtcg tgggtggcgg cgcgattgga ctggagtttg ccgattttta
     3601 ccgttgtttg ggaagcgaag tgacggtcat agagatggcg ccgcatattt tacctaacga
     3661 cgacgtaaaa gtagcgtcgc atcttagaaa ggcgctcgaa caacgcggca tgaaatttct
     3721 tgaaggcgcg gcaatcacta ctctaaaaga aaatgcaaat ggagtcagtt taacacttaa
     3781 agatagcgct ggcaaagata ccaccctcga tgcggcagtt ctcctcgtgg cgattggtgt
     3841 ccagcccaac accgaagagc tcggcatcga cgcatggggg atttgcaacg ataaaaaatt
     3901 tatcactgta gatgaaaatt tttgcactca agaaaaaaat atttttgcca ttggcgatgt
     3961 aacgcaaggg aaactgctcg cgcaccgcgc agcacaccag gggatttacg ttgccgaatt
     4021 tcttgcggga aagaatcccc gtcaaatccc cgctatcccc tcgtgtattt atacttcacc
     4081 gcaagttgct gcagtgggta tgaatgaaaa ggaactcaaa gagataaagc gggaatatga
     4141 gatgggagag ttcccatggt tagcgtcggg catggcgata gccgccggcg agcagaacgc
     4201 ctttacccgc gttttcaccg acaagaaaac cggtgaaatt ttgggtgcgc aagtggtggg
     4261 cgagaacgcc gccgaactca tcggtcaagt gtcgcttgcg atggcgtccg aacttttagc
     4321 cgaggatttt ttacatgcgg tgtttccgca tcccacgctc gcagaaacta tacccgaagc
     4381 gtttggtgtg cttatga
//
LOCUS       DAOMPD010000202         4370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8355_length_4370_cov_2.991657, whole genome shotgun sequence.
ACCESSION   DAOMPD010000202 DAOMPD010000000
VERSION     DAOMPD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8355_length_4370_cov_2.991657"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PLY93_07945"
     CDS             <1..1348
                     /locus_tag="PLY93_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRP69447.1"
                     /translation="MSHHDFPKNLSDLPAEGKLFFLTVQSLRIMSHYSVWIYFLIILW
                     RQIRLFFYSRFDFLFSDIKIISSAGEIPLRVVDIYSLSMFAAEDEGRTEDPTELRKRR
                     EREKGRVAKSAEIPAALGTLGGLIVLFISAGWVLSSIARMVKIYVGNFSSLGKIEEAT
                     IITLMVNLSRDFFIMLAPFFAAVMLLGIVGNISQTGFLFSLEPLRPKFSNISFTFNRM
                     IERVFLSKQIYVNLTKTIIKVIAIGFVSYLIISDDFLSIMKTGSVGVGEALRSLGSMS
                     FKLALILVIILLLMSIPDYIFQRSQFIESLKMTKQEVKQEFREQEGDPQIKARQRQRM
                     QEALRTQMRQAVQTADIVITNPTHYAVAIKFEFDVEGTPPKLVAKGEDEVARIIREIA
                     KANEVPIVEDKPLARFIYDNIELNQLLPENLLALLARIVAALPALRAKFNRGQAVA"
     gene            1341..3425
                     /locus_tag="PLY93_07950"
     CDS             1341..3425
                     /locus_tag="PLY93_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRP69448.1"
                     /translation="MRKWYQQNDIMLGVAVVAVVAMLIIPLPAMLLDFLLGISIMISI
                     VALLTAMFNREISDFSVFPTLLLVSTVFRLALNVSSTRLILAKGPAFEGQLIRAFGEF
                     VVGNNYVIGFVIFIILVMVQMMVITKGATRIAEVSARFALDALPGRQMALEADMSSGR
                     VTEEEGRRRNENLRREVDFYGQMDGATKFVQGDVRVGLIITAVNIVGGLVIGMVMRGE
                     GIQTALKTYTLLTIGDGLVAQIPSLLITTATGMVVTRAGAKNDLASELSTQMFQNART
                     LYITASALFAASLVPGFPKISLWVISIFLAFIAYNMREQKKIETQKQEAIQEQQKVGS
                     TERFLDELAVDPLKIEIGFNLVPLLDRKQGGTLLDRITNLRQKMAADLGMIVPPVRIS
                     DNLDLDGNEYVVLIGGIEVGRTKVFPEYLGALSNPTIRDELKPSSPDMVVTDAAFNQK
                     VILIEPEQKRDAEERGYIVIDASTMIITHLQEVFRAYASQIMGREEVKLLLDKTKQKY
                     PSVVDEVMKLFPNPGVLLKILHNLLRENVSIRNLPAILEAIADYGQRIQNAELLTDLV
                     RQRLGQQIIVPYLDKNKSLFAAVIDPQIEYDVRGNITIDERDGRVLAIDPHYREQLRS
                     VLIEAFNRMQSEGKFPVFVCGSEIRSSISEILSRDIATRSFAVIAYEEIPRETKFIET
                     TKVILEMDEVNA"
     gene            3425..>4370
                     /gene="flhF"
                     /locus_tag="PLY93_07955"
     CDS             3425..>4370
                     /gene="flhF"
                     /locus_tag="PLY93_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRP69449.1"
                     /translation="MQYFTEQGESHAAVLAKVRSKYGSGPEVLISEQRKVPAKSLWGR
                     VTGAERWEIHGAILEKNKTQSVKKKDETLDNKLKLLEEMLSRTSLRKTASGSGMDFNN
                     SGMPLKSTPELSTTPLMREVNPSLQKEILPTTNTAHEQVRRLEAEVAELKAQLKSQGG
                     PDLDLIKERELLSLYEHLLYMGFSHNFSESIAMRTRAEVPSTDYKLRKRIHARAREVL
                     AERVRTTTSAAHRIVALVGPTGAGKTTTLVKLAARLSVTQKRRVELITIDNYRIAATE
                     QLKVYARIMDIPCRVCRNAEELEGTIADSSAEYILIDTS"
BASE COUNT         1171 a         1043 c         1044 g         1112 t
ORIGIN      
        1 tatgtcacat cacgattttc ctaaaaatct gtccgacctc cccgccgagg gtaagctatt
       61 cttcttgacg gtacagagtt tacgtattat gtcgcattat tcagtgtgga tttacttttt
      121 aattatactc tggcggcaaa ttcggctttt tttttatagt cgattcgact tcttattttc
      181 tgatataaaa attatttctt ccgcagggga aatcccactg cgcgtggtcg atatttattc
      241 gctgtcgatg tttgccgccg aagacgaagg gcgcaccgaa gatcccaccg aattacgcaa
      301 gcgacgcgaa cgcgagaaag gccgcgttgc caagagcgcc gagatacccg cggcgttggg
      361 tacattgggc ggactcattg ttctatttat aagtgcaggt tgggtgctca gttcgatcgc
      421 gcgtatggta aaaatttatg taggtaattt ttcaagttta ggcaaaattg aagaagcaac
      481 cataattaca ttgatggtaa acttatcgcg cgactttttc attatgctcg cgccgttttt
      541 tgccgcagtg atgctcctgg gtattgtcgg taacatttcg caaaccggtt ttttatttag
      601 ccttgaaccg ttgcgcccca agttttccaa tatatctttt acgtttaacc gcatgattga
      661 gagagttttt ctctcaaagc aaatttatgt aaaccttaca aaaacaatca tcaaagttat
      721 agccattggt tttgtaagct acctcatcat cagcgacgat tttttgagta tcatgaaaac
      781 ggggagcgtc ggtgtgggag aagcgctgcg ttctttgggc tcgatgtcgt ttaagctcgc
      841 gctcattctt gtgattattt tgttactcat gtcgataccc gattacattt ttcaacgctc
      901 gcaatttatc gaaagtttga aaatgacaaa gcaagaagtg aagcaagaat tccgcgaaca
      961 agaaggggat cctcaaatca aagcacggca acgccagcgg atgcaagaag cgttgcgcac
     1021 ccaaatgcgg caagcggttc aaacagccga cattgttatc acaaatccaa cacattatgc
     1081 cgtcgcgata aaatttgaat tcgatgtcga gggaactcca cccaaactcg tcgctaaagg
     1141 tgaagacgaa gtggcacgta tcattcgtga gattgcaaaa gcaaacgaag tgcccattgt
     1201 cgaagataaa cctctcgcac gatttatata cgacaacata gagctcaatc aattattgcc
     1261 tgagaatctt ttagctctcc tggcgcgcat cgtggcggct ttgccggcac tacgcgcaaa
     1321 atttaaccga ggccaagccg ttgcgtaagt ggtatcaaca aaacgatatc atgttgggcg
     1381 tcgctgtcgt tgcggtggtg gcaatgttga ttattccatt accggcgatg ctgctcgatt
     1441 ttttattggg tatctcgatt atgatttcta tcgtcgcgtt actcacggcg atgttcaacc
     1501 gcgagatttc ggatttttct gtattcccca ctctgctttt ggtttcgact gtttttcgtt
     1561 tggctctcaa cgtttcttcg acgcggctca ttttagcaaa agggcctgct tttgaagggc
     1621 aactcattcg tgcgttcggt gaattcgtcg tcgggaataa ctatgtcatc ggctttgtta
     1681 tttttatcat tctagtgatg gtacaaatga tggtcatcac taaaggtgca acccgcatcg
     1741 ccgaggtgtc tgcacgtttt gcgttggatg cgctccctgg gcggcaaatg gcgctcgaag
     1801 ctgatatgag ctctggccgc gtcaccgaag aagaaggccg ccgccgcaac gaaaacttac
     1861 gccgtgaagt cgacttctac ggtcaaatgg acggtgcgac caagtttgtg caaggcgacg
     1921 tgcgtgtggg gctcatcatc acggcggtga atattgtcgg tggtcttgtg attggcatgg
     1981 tgatgcgcgg tgaaggtatc caaacggcgt taaagactta cacactcctc accatcggcg
     2041 acggtcttgt agcacaaatt ccttcgctct taatcaccac agcaaccggt atggtcgtca
     2101 cccgcgccgg cgcaaaaaac gatctcgcct ccgagctttc gacacagatg tttcaaaatg
     2161 cgcgtactct ttacatcacc gccagtgcgc tctttgcagc gtcgctcgtg ccagggtttc
     2221 ctaaaatttc actgtgggtc atcagtattt ttttggcgtt tatcgcatac aacatgcgcg
     2281 agcaaaagaa aattgaaacc caaaaacaag aggccatcca agagcaacag aaagtgggct
     2341 cgaccgaacg cttcttggat gagcttgcag tcgatccgct caaaattgaa atcggtttta
     2401 atttagtacc actccttgac cgaaaacaag gcggcactct tttagaccgg atcaccaact
     2461 tgcggcaaaa aatggccgcc gatttgggca tgattgtgcc gcccgtgcgc atcagcgata
     2521 atttagattt ggatggcaac gaatacgtcg tgctcatcgg cggtatcgag gtggggcgta
     2581 caaaagtatt tccagaatat ttgggtgctc tctcaaaccc gacaatacgc gatgagttga
     2641 aaccttcatc gccagacatg gtggtgacag acgcggcgtt taaccaaaaa gtaatcctca
     2701 tcgagcccga acaaaaacgc gacgcagaag aacgcggcta tatcgtcatc gacgcgagca
     2761 cgatgatcat cactcacctg caagaagttt tccgtgcgta cgcgtcgcaa attatgggcc
     2821 gtgaagaagt gaaactactt ttagacaaaa caaaacaaaa ataccccagc gttgtcgacg
     2881 aagtgatgaa actttttccc aacccggggg tattgcttaa aattttgcac aatcttttgc
     2941 gcgaaaatgt ttcaattcgc aatttacccg caatcttgga agcgatagcc gactatggtc
     3001 agcgcattca aaatgccgag cttctcaccg atctagtacg gcaacgtttg gggcagcaaa
     3061 ttatcgttcc ttatttagat aaaaacaaat cgctctttgc ggcggtaatc gaccctcaaa
     3121 tcgaatacga cgtgcgcggc aacatcacca ttgacgaacg tgacggtcgg gtgcttgcca
     3181 tcgatccgca ctaccgcgaa cagttgcgtt cggttttaat tgaagccttc aatcgcatgc
     3241 aaagcgaagg caaatttccc gtattcgtgt gcggctctga aattcgttca agcatttctg
     3301 aaatactctc gcgcgacatt gcaacgcgtt catttgccgt gatcgcctat gaagaaattc
     3361 cgcgcgaaac aaaatttatt gagacaacaa aagtaatatt agaaatggat gaggtgaatg
     3421 cataatgcaa tattttaccg agcaaggtga atcacacgct gcagtgcttg caaaagtacg
     3481 tagcaaatat ggtagcggcc ctgaagtctt gatttcagaa cagcgcaaag tgcccgccaa
     3541 gtcgctttgg gggcgtgtaa ccggcgccga gcgttgggaa atccacggtg cgattttaga
     3601 aaagaataaa acgcaaagcg taaaaaagaa agacgagact ttagacaata aactaaagct
     3661 gctcgaagaa atgctcagcc gcacgtcgct gagaaaaact gcgagcggtt caggaatgga
     3721 ttttaataat tcagggatgc ctctaaaatc tactcctgaa ttatcgacaa ctccattgat
     3781 gcgagaggtc aatccatctt tgcaaaaaga gattttaccg acaacaaaca cggctcacga
     3841 acaagtgcgc cgcttagaag ccgaagtggc ggagctcaag gcgcaactga aatcgcaagg
     3901 aggcccggat cttgatctaa taaaagagcg tgaattatta tcactctacg agcacttgct
     3961 ttacatgggg tttagtcaca atttttccga gagcattgca atgcgcaccc gtgccgaggt
     4021 accgtcgacc gactacaagc tgcgcaaacg tatccacgca cgggcgcgcg aagttttagc
     4081 agagcgtgtc cgcaccacca cgtcggctgc gcaccgcatc gttgcgctcg tcggccccac
     4141 gggtgcgggt aaaacgacga cactggtgaa attggcagcc cgcctttcgg taacgcaaaa
     4201 acgacgcgtc gaactcatta ccatcgacaa ctaccgcatc gctgccactg aacagctcaa
     4261 agtgtacgca cgcattatgg atataccatg tagggtatgc cgcaacgccg aggagctcga
     4321 aggtacgatt gccgacagca gcgcagagta cattcttatc gacaccagcg
//
LOCUS       DAOMPD010000203         4363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8371_length_4363_cov_3.230734, whole genome shotgun sequence.
ACCESSION   DAOMPD010000203 DAOMPD010000000
VERSION     DAOMPD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8371_length_4363_cov_3.230734"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /gene="purL"
                     /locus_tag="PLY93_07960"
     CDS             <1..1826
                     /gene="purL"
                     /locus_tag="PLY93_07960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRP69450.1"
                     /translation="YLFSRVIKGIGDYGNSLGIPNAGGEIFFHESYTRNPLVNAMTVG
                     VADIDKMASAKIPKVAGNYVVYVGAKTGRDGIHGASFASKNLSKESEEERSAVQVGDP
                     FLEKLVMEATLECIQKKLVLAIQDMGAAGLVSSSSEMAASGGVGVRLDLEKVPARETG
                     MQPFEFMLSESQERMLMVVEPARWNEVKAVFTKWELDAAIIGGTMGGNALQIFMNGEC
                     YADVPADILSKKAPRYVRETKAAPMKTGGVVLTQLDDLFKASDVSGITKLFCRLLGHT
                     NLCSRRPIYRQFDTDIGRGRIVGPGQNGCVVRVPHSNEALAMTIDGNGFYIQVDPYNG
                     AQHTVAEAVRNIAATGAIPIGLTNCLNFANPYIPENFWYFKEAVTGMSDACRTFKLPI
                     ISGNVSFYNESEDGPVLPTPTMGVVGLIENHEQAVPAAPADVFTNLELYLCGKFIPSQ
                     NASQYQYIEGRAAVGPLAELDLNAELIGAQKMVHFAKLKTLAAAIDLSAGGLLRALCR
                     MAFATYESQGAMPSLTLETQALQGLPLDTLLLGETAHTYLIAVSGAKASSVVKEATPE
                     FPVVKVGEMRIGDEKLRIANMEIPLQVLYTAWSKGLTPYLG"
     gene            1880..2221
                     /locus_tag="PLY93_07965"
     CDS             1880..2221
                     /locus_tag="PLY93_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRP69451.1"
                     /translation="MARHVDFPVDSQFTDRWSPRGFDPNHTLNEGEIGSLFEAARFAP
                     SRANEQPWIFYREKDKSSRELFLSFLTGGNAYWVKQASLVVIVCARTRFSDDNTINDH
                     ARFDTGAAGLV"
     gene            complement(2476..2742)
                     /locus_tag="PLY93_07970"
     CDS             complement(2476..2742)
                     /locus_tag="PLY93_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69452.1"
                     /translation="MEEKMAVLNAVLSIIDSESSVAKISDKAVSINKNIFERRLKLMF
                     PDQYISVKGYLQNPDFKELIEDTKTDIILYHEKIKEILNSHEQI"
     gene            complement(2748..3425)
                     /locus_tag="PLY93_07975"
     CDS             complement(2748..3425)
                     /locus_tag="PLY93_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRP69453.1"
                     /translation="MKQEIINTIKNSTFKIGTDQGAGTCVCIEPGYFLTCAHVLDSQS
                     WIELEDGVKILFQTLHKNKKYDFAKIKALARIENLAVINKQPNHKFSITEQLWVAGYP
                     AEMDNLTFMPVTVSAITTINDIPAIRFYGAAIGGFSGAPVFTKTGALVAMVTNKASNF
                     TSLFQGFISAQDTLNFYQPLLVEVVKNIKESSNLGFGYALPIHEIGKRAPTDFKIFSS
                     QEKIKEK"
     gene            complement(3719..3943)
                     /locus_tag="PLY93_07980"
     CDS             complement(3719..3943)
                     /locus_tag="PLY93_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69454.1"
                     /translation="MDELKEIIEAFKKSNTFSSEGNDLLNKSMKKFRDSSTMQNVDQK
                     MKEIKKLSEDIAEKNKELLDKLIETFTKNE"
BASE COUNT         1139 a          989 c          994 g         1241 t
ORIGIN      
        1 aatatctctt tagccgcgtc attaaaggca tcggcgatta cggcaacagc ttggggattc
       61 ctaacgcggg aggagagatt tttttccacg aaagttacac acgaaatcca ttggtgaatg
      121 cgatgactgt gggggttgcc gacatcgaca agatggcgag cgcaaaaata cccaaggttg
      181 cggggaacta cgttgtgtac gttggcgcaa agacagggcg cgatggtatt catggcgcga
      241 gctttgcttc aaaaaatttg agtaaggaat ccgaagagga acgcagcgca gtgcaggtgg
      301 gcgatccttt tttagaaaaa ctcgtcatgg aagcgacgct cgaatgtatt caaaaaaaac
      361 tagtccttgc gattcaagac atgggcgcgg cagggctcgt gagttcgtca tccgagatgg
      421 cggcgtcggg tggagtgggg gtgcgccttg acctggaaaa agtgcctgca cgggaaactg
      481 gcatgcaacc ttttgagttt atgctttcag aaagccaaga gcggatgctc atggtggtgg
      541 agcctgcgcg ctggaatgaa gtgaaggcgg tctttaccaa atgggaactc gacgctgcta
      601 tcatcggcgg aacgatgggt ggaaatgctc tccaaatttt tatgaatggt gaatgctacg
      661 cggacgtgcc tgcggatatt ctctctaaaa aagcgccgcg ttatgtgcgt gaaaccaaag
      721 cagcgccgat gaaaaccggt ggtgttgtac tcacgcagtt ggatgacctc ttcaaagcat
      781 cggatgtgtc tgggattaca aaactttttt gccgcctttt gggacatacc aatttgtgct
      841 cgcgtcgccc catttaccgc cagttcgaca ccgacattgg ccgtggccgc atcgttggtc
      901 ccgggcaaaa cggttgcgtg gtgcgagtgc cccatagcaa tgaagcgtta gcgatgacaa
      961 tcgacggcaa tggtttttat attcaagtgg atccctataa cggcgcacag cacaccgtcg
     1021 ccgaagcagt gcgtaacatt gccgccacgg gggctatccc aatagggctc accaattgcc
     1081 tcaattttgc caatccctat atccccgaaa atttttggta tttcaaagaa gcggtcaccg
     1141 gcatgtcgga cgcgtgtcgc actttcaagc tgccgatcat tagcggcaac gtgtcgttct
     1201 acaacgaatc ggaagacggg cctgtgttgc cgacgccgac gatgggtgtt gtagggctca
     1261 tcgaaaatca tgaacaagcc gtgcctgctg cgcccgcaga tgtctttacg aatttggaac
     1321 tttatctctg tggtaaattt ataccgtcgc aaaacgcgag ccaataccaa tacatcgaag
     1381 gtagagctgc agtggggcca ttagccgaac tcgatttgaa cgccgaactc atcggcgcgc
     1441 aaaaaatggt tcatttcgca aaattgaaaa cacttgcagc ggcaatcgat ctatcggcgg
     1501 gtgggctttt gcgcgcgctt tgccgcatgg cgtttgccac ttacgaatca caaggagcga
     1561 tgccgtcgct cacgttggaa acgcaagcgc tgcaaggttt acctctcgac accttgcttt
     1621 tgggtgagac agcgcacacg tatttgattg cggtgagtgg agcgaaagcg agtagcgtcg
     1681 ttaaggaagc gacgccagag tttcccgtag ttaaagtggg agagatgaga ataggtgacg
     1741 aaaagcttcg tatagcgaat atggaaatcc cgcttcaagt cttatacaca gcgtggtcga
     1801 aaggattgac tccttattta ggctgattgt ttgagcgcct cacgatgcag aacacaaatg
     1861 acacggtaga ataatttata tggcacgaca cgtagatttt ccagtcgact cccaatttac
     1921 cgaccgttgg tcgccacgcg ggtttgatcc caaccataca ctcaacgaag gagagatcgg
     1981 ctcgcttttt gaagccgcac gctttgcgcc ttcgcgggca aatgagcagc catggatatt
     2041 ttatcgtgaa aaagataaat cgtcgcgtga gcttttttta agcttcttaa ccggcggcaa
     2101 tgcgtactgg gtaaaacagg cgagccttgt ggttatcgtt tgtgcccgga caaggttttc
     2161 tgacgataac actataaacg accacgcgcg ttttgacacc ggcgcggcgg ggttggttta
     2221 gccccgagta gccctcaccc cccggccccc tctccctcaa gggcgagggg gagaaagagc
     2281 cccaccacgg ccagcacagg gtgctggcca cccctcccca ttgacatgga gagggatttt
     2341 tgtgcgtaga gacgcgattc atcgcgtctc tatgttattt ccccatttgc caaaggcaaa
     2401 tgccccaatg tccctcttcg cgttggtggc gagagtaatt tcagaatttt ctcatcgaac
     2461 tttaaaagat ttagttcata tttgttcatg tgaattgagt atttctttaa tcttttcatg
     2521 atataaaatt atatcggttt tagtatcttc aatgagctct ttaaaatcag gattttgaag
     2581 ataacccttg actgagatat attgatcggg aaacatcagt ttaagtctcc tctcaaaaat
     2641 atttttatta atactcactg ctttgtcact aatctttgct acagatgact cagaatcaat
     2701 aatcgataac acggcattta agaccgccat tttttcctcc ataaatgcta tttctctttt
     2761 attttttctt gagaagaaaa gattttgaaa tccgtaggtg cacgtttgcc tatctcatga
     2821 ataggtaatg catatccaaa accaagattt gaactttctt tgatattttt cactacctca
     2881 acaagcaatg gttgataaaa atttagtgtg tcttgagcac tgataaaccc ctgaaacaaa
     2941 gaggtgaaat tagaagcctt attggttacc attgctacga gggcacctgt tttagtaaat
     3001 actggtgcgc ctgagaaacc gccaatcgcc gcaccataga agcgaattgc cggaatatcg
     3061 ttgatggttg ttatcgcaga cacagttact ggcataaaag tcaagttatc catttctgca
     3121 ggatagcctg ctacccacag ctgttcggtg attgaaaatt tatgattggg ttgtttattg
     3181 ataactgcta agttctctat tcgagcgagg gcctttattt tagcaaaatc atatttttta
     3241 tttttgtgaa gtgtctgaaa tagtattttc acaccatcct caagctctat ccaactttgt
     3301 gagtccaaga cgtgggcgca tgtgagaaaa taacctggtt cgatacagac acatgttccg
     3361 gctccttggt ctgttccgat tttaaatgta ctatttttaa tagtattaat tatttcttgt
     3421 ttcatgttaa cctccatgat ttatattaat aaaatctgtg tgtgaattta ttcgtgaatt
     3481 tttattacag gcgcgtcaat atcgcacttg ccataattat ctactctcca aatcttacat
     3541 aatctatcca gccgtacaca ccgacatcag ccttaaagca gagagtcaaa ggcgtgcatg
     3601 acgtacccgt cgatggtgcg tcatggctca atatacttgt atgcaatcgt atcaaaagca
     3661 ggtaatagtt gacctgagtt tgaaagtact tgcacatcaa aaatgaagtt aaaaaatgct
     3721 attcattttt agtgaaagtt tctatcaatt tatcaagtag ttccttgttt ttttctgcta
     3781 tgtcttcact caatttttta atctctttca tcttttggtc tacattttgc atcgttgaag
     3841 agtctctaaa tttcttcata gacttgttaa gcaagtcatt tccttcgctg gaaaaggtat
     3901 tgcttttttt gaatgcttca attatttcct ttagttcgtc catatgtaaa atctcctaag
     3961 ctcttatttt tgttttgtgt actaaactat tttagcacct atactgctgt aacagtattt
     4021 tttgtttcta aatttttcac ctccccccaa actaaatctt cgcataaact attatgccac
     4081 acacaaaacc tgtgcctcga tacaaatttg cctacggcaa atttactcgg cacaggctcc
     4141 tacctctatg ggtaaatagg agcctggccc gagtgtccct caccttttct gtgaaaaggt
     4201 gagggatgta tcgagggtca ggcgccatct actcactata cttcaccaac acgacattct
     4261 cgttgctgcc ggtagcacta cccgccacgg ataccccact gccataacta tagctatcac
     4321 tacctctttg ataacctgcc gcgtaaacac cactcgagtt atc
//
LOCUS       DAOMPD010000204         4357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8394_length_4357_cov_2.257090, whole genome shotgun sequence.
ACCESSION   DAOMPD010000204 DAOMPD010000000
VERSION     DAOMPD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8394_length_4357_cov_2.257090"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLY93_07985"
     CDS             <1..493
                     /locus_tag="PLY93_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRP69455.1"
                     /translation="RSESSWCDSKPFDAYRVTQRREVRSYNKVADGETCSTARVDRGD
                     GSFKKEKRCTTKYKEVPVYAQKCYYTVNRWGYERDIQSRGGLNDTPVWPAVNIRAGNC
                     LGCERESGRSEAFVLHLVEVKKADKKHNCSYDEAKWRSIPDKAIKKIKVRLLGGAKCD
                     TLQ"
     gene            complement(519..>1283)
                     /locus_tag="PLY93_07990"
     CDS             complement(519..>1283)
                     /locus_tag="PLY93_07990"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRP69456.1"
                     /translation="DDVAIGGKGFERRGVGEGGIVHANCRSFVGAYPLAVRHSLTLAE
                     LALYLNATENWRASIEILKLKNWERGQLYPDTGALWVQPSPNMPTVDTAVVYPGTCLF
                     EGTNVSEGRGTTRPFEIIGAPFIDAKKYTEALNTLNLPGVYFRPVYFKPTFHKFQDSV
                     CGGAFVHVTDRARFESFYTGLMMVRTAHDLWPHEFDWRREPYEYVNDVLAIDLLTGAP
                     MFRLAVEKGDALTTYRASYEEEGRAFARQAKEFYLY"
     assembly_gap    1284..1293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1294..1744)
                     /locus_tag="PLY93_07995"
     CDS             complement(<1294..1744)
                     /locus_tag="PLY93_07995"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRP69457.1"
                     /translation="MAVTLGAEVLLQEADRYLKGKKFALLVNQSAVTKGGLYLFEALL
                     KKGYKPEKIFAPEHGLFGVEQDQITIDDAVDAFTGVKAVSLYGQTFAELHPHATNFEG
                     IDTLVVDIQDIGCRYYTYAYTMAFCIEVAARVGVRVLVCDRPNPLGGI"
     gene            complement(1744..2493)
                     /locus_tag="PLY93_08000"
     CDS             complement(1744..2493)
                     /locus_tag="PLY93_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRP69458.1"
                     /translation="MNAFLLAAGLGTRLRPLTDKVAKPALPVAGLPMLAYSLNFFFEA
                     EVKKLAVNTHHAPQTIHRALKKIEMPWQLHISHEEKLLDTGGGVKKCEAFLAGERVLL
                     ANGDIVSDVDIARLIRFHEARKAKLTLVVVPHPNANAIAPITIRANDAIVDINRTFDK
                     TNTGSHLYAGVAIFEPILWQYLKSEPSSIVYTAYTALIAAGEKVCAYVHDGIWFDCGI
                     IESYHTANRALESNAWHWQKVLHPNIKNAFA"
     gene            complement(2490..3017)
                     /locus_tag="PLY93_08005"
     CDS             complement(2490..3017)
                     /locus_tag="PLY93_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRP69459.1"
                     /translation="MQKLMFEFDFYIEHTLLGYWQQALTESDLSRIRNQFLAVAEKLA
                     AYPHKVFTHRDYHSRNVMLATDSSTQYIIDFQDARLGLVQYDLCSLLYDAYAQLESSM
                     RSALVLFAYEAGREIHRQTHDEFMHYFYLSAYQRTIKAMGTFGYQASQGRRDFAAYLE
                     PAQAMVEEIVKVYSL"
     gene            complement(2980..3426)
                     /locus_tag="PLY93_08010"
     CDS             complement(2980..3426)
                     /locus_tag="PLY93_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69460.1"
                     /translation="MHETIEAQFQNALAHAQMATLQSLSPLVPEASTRRYFRLFLEDD
                     STCVGVFENPETAKKTMPRVVAVQRFLENGGIAVPRIYFTDTHAGVMLQQDLGDVSLN
                     TALKAHPKKTDSKYVRLATPQRRRRMPRVSSRIRRAKTHVRVRFLY"
     gene            complement(3428..4168)
                     /locus_tag="PLY93_08015"
     CDS             complement(3428..4168)
                     /locus_tag="PLY93_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69461.1"
                     /translation="MIYYHTTHPTRVAGKSLDNGLAHGWYRIGQSFSTTDLIVVQEQL
                     LPVFWLRIDLKRYTPSRSARRILKKNQDFSVAILPFKITPEIENLFAAYRAGIDFSMS
                     ETVAEYLLDNEQYNAYDTYLIEVRDKDRLVAVGYFDVGKKTNTGILHFYDHALGDFSL
                     GKFLFLLELEYGLTHGFDYYYMGYLCKGDTKFDYKLFADRNSTEVYLRALNRWLPYNA
                     MDKKISRWGEKIIDTCRRSYRLVPTEEQ"
     gene            complement(4168..>4357)
                     /locus_tag="PLY93_08020"
     CDS             complement(4168..>4357)
                     /locus_tag="PLY93_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRP69462.1"
                     /translation="LDEQNVGRFLTLLDDFSRNTQFILITHNKKTMGKAQVLYGVTMN
                     EPGVSTLLSVELSKKTVL"
BASE COUNT         1075 a         1069 c         1113 g         1090 t
ORIGIN      
        1 acgctccgaa tcgagttggt gcgactccaa gccttttgac gcgtatcgcg ttacccaacg
       61 ccgcgaagta cgctcttaca acaaagttgc cgatggcgaa acgtgctcca ctgcacgcgt
      121 cgaccgtggc gatggctcgt ttaagaaaga aaaacgatgc accacaaaat ataaagaggt
      181 gcctgtatac gcgcagaaat gctactatac agttaaccgc tggggttacg aacgtgacat
      241 ccaatcacgc ggcggactaa atgacactcc tgtgtggccg gcggtaaaca tacgcgctgg
      301 aaactgcttg ggttgtgaac gcgaaagtgg gcgtagcgaa gcctttgtgt tacaccttgt
      361 ggaagtgaag aaagcggata agaaacacaa ctgtagttac gatgaagcaa agtggcgctc
      421 tattccagac aaggcgatca aaaaaattaa agtgcgcctt ttaggcgggg caaagtgcga
      481 tacgctacaa tagcgccgta cgctgtttac tcaaaacgtc agtacaaata aaactctttc
      541 gcctgccgtg caaacgcgcg tccttcttct tcgtacgatg cgcggtaagt ggtgagcgca
      601 tcgccttttt ccacagcaag gcggaacatg ggcgcgccgg taagtaggtc aatcgccagc
      661 acatcgttta cgtactcgta cggttcgcgc cgccaatcaa attcgtgcgg ccataaatcg
      721 tgcgctgtgc gaaccatcat aaggccggta taaaaacttt caaaccgcgc tctatccgtg
      781 acatgcacaa acgcgccacc gcatacacta tcttggaatt tatggaacgt gggtttgaaa
      841 taaactggcc taaaataaac tccaggcagg ttgagtgtgt tcagcgcctc tgtgtatttt
      901 ttggcgtcga taaagggtgc accgatgatt tcaaaagggc gcgtagtgcc gcgcccttcg
      961 gagacattcg tcccctcgaa aagacaagtt cccggatata caaccgcggt gtccaccgtc
     1021 ggcatgttgg gcgatggctg cacccacaat gctcctgtgt ctggataaag ctggccgcgt
     1081 tcccagtttt taagttttag gatttcgatc gaggcgcgcc aattttcagt ggcatttaaa
     1141 tagagcgcaa gctccgccaa tgttaaacta tgtcgtacgg caagaggata cgcacccacg
     1201 aacgaacggc agtttgcgtg gacaatccct ccttcgccga caccacggcg ctcgaagcct
     1261 ttgccgccga ttgcgacgtc atcnnnnnnn nnncaatgcc acccaacggg ttggggcgat
     1321 cgcaaaccaa cacgcgcacc cccacacgcg ctgctacctc gatgcaaaac gccattgtgt
     1381 aagcgtatgt atagtaacgg caaccgatgt cttggatatc gacaacgagg gtgtcgatgc
     1441 cttcaaagtt tgtggcgtgg gggtgtagct cagcaaaagt ttggccgtag agactcaccg
     1501 ctttcacgcc agtgaacgcg tcgacggcat cgtcgatggt aatttggtct tgctcgacgc
     1561 caaagagtcc gtgttcggga gcaaaaattt tttctggttt atatcctttt tttaggagtg
     1621 cttcaaatag ataaagaccg cctttggtga ctgcgctttg atttactaac aacgcaaatt
     1681 tttttccttt taagtatcga tccgcctctt gaagaaggac ttcagcgccg agagtaacag
     1741 ccattatgca aacgcgtttt tgatattggg gtgtaaaact ttttgccagt gccatgcgtt
     1801 gctttcgagc gcgcggttgg cggtgtggta actttcgatg atgccacaat cgaaccagat
     1861 gccgtcgtgc acgtacgcgc acactttttc gcccgccgca atgagcgcgg tgtatgcagt
     1921 gtacacgatg cttgagggtt cgcttttcaa gtattgccac agtatcggtt caaatatcgc
     1981 cactcccgca tagagatggc tacccgtatt cgttttatcg aatgtacggt taatgtccac
     2041 gatggcatcg tttgctctaa tcgtgatggg agcaatcgca tttgcattcg gatgcggcac
     2101 cacaacgaga gtgagttttg ccttgcgcgc ttcatgaaaa cgaatgagtc gcgcaatatc
     2161 gacatcggac acaatgtcgc cgtttgccaa cagcacgcgc tcgcccgcta aaaatgcctc
     2221 acacttttta acgccaccgc ctgtatccaa caatttctct tcatgagaaa tatgaagctg
     2281 ccacggcatc tcgatttttt tgagcgcacg gtggattgtc tgtggcgcgt ggtgggtgtt
     2341 aaccgcaagt ttttttactt cggcttcaaa aaagaaattg agcgaatacg cgagcatcgg
     2401 cagccccgcc acaggcagtg caggttttgc aactttgtcg gtgagcggtc ttaggcgcgt
     2461 gcccaaaccg gcagcaagga ggaatgcgtt cataaggaat ataccttcac aatctcctcc
     2521 accatcgcct gcgccggctc aaggtatgcc gcaaagtcgc gcctaccttg cgaggcttga
     2581 taaccaaatg tgcccattgc ctttatcgtg cgttggtacg cgcttaaata gaagtagtgc
     2641 ataaactcgt cgtgcgtttg ccggtggatt tctcgacccg cttcgtacgc gaacaatact
     2701 aatgcgctgc gcatggaaga ttcaagctgg gcgtacgcat cgtagagaag tgagcataaa
     2761 tcgtactgca caagccccag ccgcgcgtcc tggaagtcga tgatgtattg agtcgaggag
     2821 tctgtggcaa gcatcacatt gcgcgaatgg taatcgcggt gggtgaaaac tttgtgtggg
     2881 tatgcggcga gtttttcggc aaccgctaaa aattggtttc ggattcggga taaatccgac
     2941 tcagtgagcg cttgttgcca ataacccaac agcgtgtgtt caatataaaa atcgaactcg
     3001 aacatgagtt tttgcacgtc gaattcgaga cgaaacgcgg ggcatgcgcc gtcgtctttg
     3061 aggcgttgcc aaccgtacat attttgaatc tgtttttttg ggatgcgcct tgagcgcggt
     3121 gttgagactc acatcgccca agtcttgttg cagcatcaca cccgcgtgcg tatcggtgaa
     3181 atagatacgc ggcacggcaa tgccgccgtt ttccaaaaag cgttgcaccg ccacaacgcg
     3241 cggcatagtt ttttttgccg tctctggatt ttcaaaaaca ccgacgcaag tggagtcatc
     3301 ttccagaaac aaccggaagt agcggcgcgt cgacgcttcg gggacaagtg gggagagcga
     3361 ttgcagtgtc gccatttgcg catgcgccaa cgcgttttga aattgtgctt cgatagtctc
     3421 gtgcatgtca ctgctcttcg gtgggtacaa ggcgataact acggcggcaa gtgtcgataa
     3481 ttttttcacc ccaccgcgat atttttttat ccattgcgtt gtatggcagc cagcggttga
     3541 gtgcgcgcaa atacacttcg gtggagttgc gatcggcaaa aagtttgtag tcaaattttg
     3601 tgtcgccttt gcacaaataa cccatgtagt aataatcaaa cccgtgcgtt aaaccatatt
     3661 ccaattcgag caaaaaaaga aatttaccca aactgaaatc accgagcgcg tggtcgtaaa
     3721 aatgcaagat gccggtgttg gtttttttgc ccacatcaaa gtaccccact gccaccaaac
     3781 gatctttgtc gcgcacctcg atgaggtatg tatcgtacgc gttatactgt tcgttgtcga
     3841 gcaaatactc ggcgacggtt tcagacatgg aaaaatcgat acccgcgcgg tatgcagcaa
     3901 ataaattttc aatttcggga gttatcttaa acggtaggat tgccactgaa aaatcttgat
     3961 tcttttttaa tatacgacgt gccgaccgtg aaggggtata gcgtttcaaa tcgatgcgca
     4021 gccaaaaaac cggcagtagt tgttcttgaa caacgattaa atcggtcgtt gaaaaacttt
     4081 ggccaatgcg gtaccacccg tgtgcaaggc cgttatccaa gcttttgcca gcgacgcgag
     4141 tgggatgcgt ggtgtggtaa taaatcatca gagcacggtt tttttgctca gctcgacgct
     4201 caagagagta ctcactccgg gctcgttcat tgtgacaccg tagagtacct gcgctttacc
     4261 catcgttttt ttgttgtgcg tgatgagaat gaattgggtg ttacgtgaaa aatcgtcaag
     4321 tagggttaaa aaccgcccca cgttttgttc gtcgagc
//
LOCUS       DAOMPD010000205         4356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8397_length_4356_cov_2.434783, whole genome shotgun sequence.
ACCESSION   DAOMPD010000205 DAOMPD010000000
VERSION     DAOMPD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8397_length_4356_cov_2.434783"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLY93_08025"
     CDS             complement(<1..495)
                     /locus_tag="PLY93_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69463.1"
                     /translation="MRVKFLTSTASAVLTCCTLIGFVNPSPLHAGSCCGGGTAGALIM
                     PKSAHVMLAVSGDFEKYSGYFDRTGNYMSDPPHSDLQQYRLNGGVAVRLASHWQASFA
                     SGYVFNANKYAGLASNTNGLADSTLGVLYETFDNIRCVWRVRKWEDLIPAVYLGAQAT
                     IPTGI"
     gene            complement(500..1231)
                     /locus_tag="PLY93_08030"
     CDS             complement(500..1231)
                     /locus_tag="PLY93_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRP69464.1"
                     /translation="MKTTYTATLMSGSTQVVGKNVVQIKITGNDGVGVSHLDPTIEAE
                     MDMGTMKHTTPVASVTDNGGGVYTAEVYYIMATSGSMHWTLKVLDKSGFEIADRIPVT
                     VNGMMTARVSVSSASDQFNNMGTATNRPYFVFIDTVTGTAGSHTIKIFAATRKSMMMH
                     PALVTGLTLANASSTNWNVTSIGIELSIDSGATWITTPTNDGGGKFSFTSVAGLSTAA
                     SQDLMFKVTVNGNTIGQGTLKAQIP"
     gene            complement(1321..1779)
                     /locus_tag="PLY93_08035"
     CDS             complement(1321..1779)
                     /locus_tag="PLY93_08035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRP69465.1"
                     /translation="MAVASISTSAFAMEKITLPLLPPVKAVNNNTLPDYAGKPILVVF
                     WAVWCAPCMAELKDVEKFYQEKEIAVVGISVESPQKKAENFLKRVGITFDNFLDENSA
                     FADNVQITGVPTLLILDKHGNTIWMKSGYSTFPEKELRGAIQKIKLIKKE"
     gene            complement(1868..2257)
                     /locus_tag="PLY93_08040"
     CDS             complement(1868..2257)
                     /locus_tag="PLY93_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69466.1"
                     /translation="MRSILRYRLLHILGLMLFLVQMMDARAIYAGVTQSKYLICPHTK
                     AKFIRGTPCPCGHEHSKSHKGTRIVDEHADCNDDEDGLVRTPAFEAFIFIFDSEFLPR
                     FEAHEELFHPHTHIIFGLEVPPPDAPS"
     gene            2335..4152
                     /locus_tag="PLY93_08045"
     CDS             2335..4152
                     /locus_tag="PLY93_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69467.1"
                     /translation="MAQAEYEFDKTRSAIGADDLDGSERKEMLERFKSVGGQVLKEKS
                     LAEKQADARRAKEAKMGSASPRSVGGGALQETKLPSELRREKEREESEKIQRARQEYE
                     KALKKISGPGARFMIRLRCFLSGVSPFSGKAIKPSFVHFLNLEIKQALIEFNLIGNDL
                     FVQRPSIGKKIMHNLDAKNPLLMEALEYAHHIYNSEYFAPFISAAQTNSQVSLEAGAA
                     SIKSIYRALYLLYPFQETFRKAMGYALDIFIPEAQAAKLSQESLQGMAVKQKRFQANV
                     KLVFQTAFPKLFNLVCLVDGTDYPPYSPLLEKALQVTQNDKLGQRQKGEGALAESMNT
                     ENAATEQAGATEEEQEKTKEPENKSEHADKAEYRYGMSLMLLPPALVAKHDKTKRMLE
                     KVSLNDRILISYLYFLEFDQEYSFVLTTNKIHINVDYSGGVKSDHKKQMADLYNDSRA
                     IIKAYEKYEEMVQEYKAQIARKSSNYIEKSKLDDKAKGRADMEARNTRGLIRNFMDNV
                     TKKMALLIQDMRGAKQIVGNMDEPIKFDGAEKDKRLNGQPVKKCITDAYSFAFVFKER
                     LANGDLFPLTPMTEEEMIASFGISFGDVAVKSPTENNDF"
     gene            4152..4328
                     /locus_tag="PLY93_08050"
                     /pseudo
     CDS             4152..4328
                     /locus_tag="PLY93_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020764084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
BASE COUNT         1205 a          987 c         1069 g         1095 t
ORIGIN      
        1 tattccagtg gggatggtcg cttgcgcacc caagtacacc gctgggatga ggtcttccca
       61 tttacgcacc cgccacacac aacgaatgtt gtcgaaggtc tcgtacaaga cgcccaacgt
      121 gctgtcggca agcccgttgg tgttcgaggc gaggcctgcg tatttattcg cgttgaacac
      181 ataccccgag gcgaaagacg cttgccaatg ggatgccaag cgcaccgcaa cgccgccgtt
      241 taagcggtat tgctgcaaat cggagtgtgg cggatcgctc atatagttac ccgtacggtc
      301 gaaatagccc gagtattttt caaaatcgcc cgatacagca agcatgacat gcgccgactt
      361 aggcatgatg agcgcacccg ccgtgccgcc gccgcaacac gaccctgcgt gcaaggggga
      421 tggatttacg aaaccaatga gggtgcagca agtaagtacg gctgacgccg tacttgttaa
      481 aaattttact ctcatggcgc taagggattt gagccttcaa cgtaccttgg ccaatggtgt
      541 tgccattcac ggtcacctta aacatcaagt cttgcgacgc agcggtgctt aaacccgcaa
      601 cgctggtgaa tgaaaatttt ccaccgccgt cgtttgttgg agtggtaatc cacgttgcac
      661 cgctgtcgat ggaaagctca atgccgatgc tggtgacatt ccagtttgta ctggaggcgt
      721 tggcaagggt aagccctgtt acaagcgcag gatgcatcat catcgactta cgcgtggcag
      781 caaaaatttt gatggtatgg ctccctgctg tacccgtaac ggtgtcgata aagacaaaat
      841 acgggcggtt ggttgcggtg cccatattgt taaattgatc gctggcgcta ctcaccgaaa
      901 cacgagcggt catcataccg tttacagtca ctggaatgcg atcggcaatc tcaaaaccac
      961 tcttgtctaa aactttaagt gtccagtgca tactacccga tgtcgccata atataataca
     1021 cctcggcggt gtagacgccg ccgccgttat cggtaaccga cgcgaccggc gtggtgtgtt
     1081 tcattgtgcc catatccatt tcggcttcga tggtggggtc taaatgggaa actcccacac
     1141 cgtcgttacc ggtaattttt atttgcacaa catttttgcc tacaacctgt gtggagccac
     1201 tcatgagcgt agcggtatat gtcgttttca aatcatcgct ctgcgcgcac gcagtgagca
     1261 gggctacact tgccaataca agtatagagt taaatttttt tattgtataa gttttcatag
     1321 ttattccttc tttattaatt ttattttttg aatcgctccc cgaagttctt tttcggggaa
     1381 agtggagtaa ccgcttttca tccaaatggt gttgccgtgt ttgtctaaga taagaagcgt
     1441 cggcacgcct gtaatttgaa cgttgtcggc gaacgcgcta ttttcatcca agaaattgtc
     1501 gaaagtgatg cccacacgct tcaaaaagtt ttcggctttt ttctgtgggc tctccacaga
     1561 aatccccacg acggcgattt ctttttcttg ataaaatttt tctacatctt taagctcggc
     1621 catgcacggt gcgcaccaca ccgcccaaaa aacgacaaga atgggcttgc ctgcgtaatc
     1681 gggtagcgtg ttattattga ccgctttcac gggtggcagt agaggtaggg ttattttttc
     1741 catcgcaaaa gcagatgtac ttattgacgc tactgccaca gtggctctta aaatccatcg
     1801 ggacatagtt actcctttga attaaaccaa acccaaagcc gctatgcgac ttttggtttt
     1861 gggaagctca agaaggagcg tcggggggtg ggacttcgag tccaaaaatg atatgagtgt
     1921 ggggatgaaa taattcttcg tgcgcttcaa agcggggtag aaattcgcta tcaaaaataa
     1981 aaataaacgc ctcgaaggca ggtgtgcgta caagaccgtc ttcgtcgtcg ttacaatcgg
     2041 cgtgttcgtc gacaatgcgt gtgcctttat gcgactttga gtgttcgtgg ccgcaagggc
     2101 agggggtacc tcggataaat ttagctttgg tgtgcggaca aatgagatat ttggattgag
     2161 tgacacccgc atagatagcg cgcgcatcca tcatttgcac aagaaagagc attaacccta
     2221 atatgtgcag taaacggtac cgtaaaatcg accgcatagg gtcgaaggcg ggatatgaag
     2281 cagtgcgtca atcttaattg acaacgcatt tcggcgtcgc gttcgtcttt tgatatggcg
     2341 caagcagagt atgaatttga taaaacacga tcggcgattg gcgccgacga cttagacggt
     2401 tctgaacgta aagaaatgct cgaacgcttt aagtcggtgg gcgggcaggt cttaaaagaa
     2461 aagagccttg ctgaaaaaca agccgacgcc cgccgcgcta aagaagcaaa aatgggtagc
     2521 gcttctccgc gctctgttgg tggcggtgcg cttcaagaaa caaaactccc gtcagagttg
     2581 cgccgtgaaa aagaacgtga agaatcagaa aagatacaac gtgcacggca agaatacgaa
     2641 aaagctctca aaaaaattag cggtcctggt gcacgcttta tgattcgttt acgttgtttt
     2701 ttgtcggggg tgtcgccgtt tagcggtaaa gcaattaaac cgtcgtttgt gcatttctta
     2761 aatttagaga taaagcaagc gttgattgaa tttaatttaa ttgggaacga tttatttgtg
     2821 caacgaccgt cgattggtaa aaaaattatg cataatctcg atgcaaaaaa tccactgctt
     2881 atggaagctt tggagtatgc gcaccatatt tacaacagcg aatactttgc accgtttata
     2941 agcgccgccc aaaccaactc gcaagtttcg ctggaagcgg gggcagcttc gattaaaagt
     3001 atctaccgtg cgctttatct tttgtatccg tttcaagaaa cattccgcaa agcgatgggt
     3061 tatgcactcg atatttttat ccccgaagca caggcggcga aactctcgca agagtcgctg
     3121 caaggcatgg cggtaaagca aaagcgattc caagcgaatg tcaaattagt ttttcaaact
     3181 gcctttccca aactttttaa tttagtttgc ttggtcgatg gcaccgacta tccgccgtat
     3241 tcaccgcttt tagaaaaagc gctacaagtg acgcaaaacg acaagttggg tcaacggcaa
     3301 aaaggtgaag gcgctcttgc agaaagtatg aacacagaaa acgctgccac tgaacaagca
     3361 ggtgcaacag aggaagaaca ggaaaaaact aaagaacctg aaaataagag cgagcatgcc
     3421 gacaaagccg agtatcggta tggtatgtcg ttgatgctgt tgccgccggc actcgtggcg
     3481 aaacacgata aaacaaaacg gatgcttgag aaagtgtcgc tcaacgaccg cattttaatt
     3541 tcgtatttat attttttaga atttgatcaa gagtattcgt ttgttttgac cacgaataaa
     3601 atccacatta acgtggatta ctccggtggt gtgaagagcg atcataaaaa acagatggcc
     3661 gatctttaca acgactcgcg cgccatcatt aaagcgtatg aaaaatacga agaaatggtg
     3721 caagagtata aagcgcagat tgcgcgtaaa tcgagcaact acattgaaaa atcaaaatta
     3781 gacgataaag ccaaaggccg cgccgatatg gaagcccgca acacgcgcgg tctcatccgt
     3841 aatttcatgg ataacgtgac caaaaaaatg gcgcttttga tccaagacat gcgtggcgca
     3901 aaacaaatcg tcggtaacat ggacgaaccg ataaaattcg atggtgccga gaaagataaa
     3961 agactcaatg gtcaaccagt aaaaaagtgc atcaccgacg cttatagttt tgcctttgtt
     4021 ttcaaagagc gtttagctaa cggcgatctt ttccctctca ctccaatgac cgaggaggaa
     4081 atgatagcct ctttcggtat ttcattcggc gatgtggcag tgaagtcgcc aacagaaaac
     4141 aacgattttt aatgaaaaca ttggcagtta tccccgcgcg ttttgcgtcc acgcgttttc
     4201 cgggtaaacc tctggctctc attcacggta aacccatgat tgaatgggtg taccgcgcgg
     4261 cacaaaaatg caaagcgctc gcgcacattg ttgtagccac cgacgatgag cgtatagtaa
     4321 aagcggtgta agcagtcagc ggagatgtga ttctca
//
LOCUS       DAOMPD010000206         4325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8465_length_4325_cov_3.115457, whole genome shotgun sequence.
ACCESSION   DAOMPD010000206 DAOMPD010000000
VERSION     DAOMPD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8465_length_4325_cov_3.115457"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..105
                     /locus_tag="PLY93_08055"
     CDS             16..105
                     /locus_tag="PLY93_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69468.1"
                     /translation="MRDAVKAGRAGFLAGAIPPKLYATASSPI"
     gene            complement(114..494)
                     /locus_tag="PLY93_08060"
     CDS             complement(114..494)
                     /locus_tag="PLY93_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69469.1"
                     /translation="MEILLKSGAVLLLVSLALAWLLVGVKYLGLFPKIIKNQKHLLSA
                     HIDYILMAILNWVVYHIVGPHGNEHMAHLIIIGSFWNPFLFLVMAVKPDIKKSPFSPF
                     GMFSSVSFIFTTTGYAWAAITLLV"
     gene            complement(497..1291)
                     /locus_tag="PLY93_08065"
     CDS             complement(497..1291)
                     /locus_tag="PLY93_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRP69470.1"
                     /translation="MKLKFLIVFLFAVSFLAAEKTTKTSTDKTSEEEQLQIVEVKRGD
                     TLAKISKKYLEDPSQWPALLKYNKIANPNLIQPGMKLKVPASLSKKPAAVVIYKSGSA
                     QYTRAQERVWKEVLLKLGLYPEDQVKTGAHSLVHLQLTNQAILRIQPQSFVIVNKVGK
                     SNKELIFTLQNGRLQAQAGNMHSLGGRLAIRTPVAVASVRGTIFELTSTENDTGLACH
                     EGQVAVSAQKVTVEVPAGMGTYVEKGKAPMKPFKLPLPPQVKPAET"
     gene            1349..2095
                     /locus_tag="PLY93_08070"
     CDS             1349..2095
                     /locus_tag="PLY93_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69471.1"
                     /translation="MTTTPTTPRLFLQSVAFALFAAIIYFLINRFTGDKPGVYLVTFF
                     DHSIPFTPSAVFGYALGFTFIFIPVLFFQHSAFLKKVLWGFILCTALAAVLYFVFPSR
                     LPRHGVPTQEKFFYWCVALIYTLDRPVAAMPSLLALYGFLSAWTILPLSRRIGWGILA
                     AGVIFSAAAVALRQQLTAHILLAALVAYAVFLGIIKPTLAINKKAIKESAVPLLTVGK
                     RLALFYGIIILAAYLLFIIGVRFPLALPEN"
     gene            2104..3714
                     /locus_tag="PLY93_08075"
     CDS             2104..3714
                     /locus_tag="PLY93_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP69472.1"
                     /translation="MKQKTQTRNPVGWGYIEEAITPREKEAVLKNFHILFPKGQFVQN
                     TPLTANQITLTPTRIEVNPTLAHIVSIAQESRLLHAVGRSFRDLAKLREVHPLKASDA
                     VATPTTQEDLLRVLEWASQKNFAVIPFGGGSSVVGGVNPEGMDTFNGVITVDLQSMNR
                     VLDVNRTELVVHAEAGILGPALDAALKLHKMHTRYSPQSFHHSTLGGWIATRGAGHNA
                     TVYQKIETRVQSVEGVLANGRTFTTRPLSATSVSIDPQSYWTGSEGMLGFITAARLRV
                     YPLPEHRAFRAFAFPTFAHALDTARHIAQSELFPVQVRVLDEDENAQTARLAGVEATG
                     EALLILGDESTLPIVEMRLDAVGKIALECAGKKSEGASRLLREWVRMFFRQPYLRDML
                     IDHNVIVDTFETSITWAKALEFHSRVKAVAKEALIHECGAGHVGCRVTHIYSDGLCLY
                     YSFYGLGKRGALTKAWQRIKHRVSAEVIAAGGTISHHHAMGRDHRAYAHDEFSKAHRS
                     ALHDLKKSLDPQGIMNPGLCVSPPPAGE"
     gene            complement(3689..>4325)
                     /locus_tag="PLY93_08080"
     CDS             complement(3689..>4325)
                     /locus_tag="PLY93_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69473.1"
                     /translation="GNAARLAKWVSEIACETPHDALFHIANPAVFAKEDLPESLAERI
                     DAVLSATNTRAWLFEEGKLPPLIERMNYFKYFCLFERYSDGVRCFAFLGIFRHSRNFR
                     LFYARALFMSGMLHDAWAEIASLLEKYPSDRALLNEAGIYAHKMTRYDEASEIFARAR
                     SLYPDDATLAYNEAVFTEDFSRVQVAEKWSRVEKLNSWAEPERIIPPPVVD"
BASE COUNT         1069 a         1139 c         1018 g         1099 t
ORIGIN      
        1 atcatggccg ccgcgatgcg cgatgcggtc aaagcaggtc gtgcaggttt tttagccgga
       61 gcgatacccc ccaaacttta tgcaaccgct tcgagtccta tataaaattt cgattagacc
      121 aacagggtaa ttgccgccca cgcatagccc gtggtagtga aaataaacga tacgcttgaa
      181 aacatcccaa aaggagagaa cggcgatttc ttgatgtctg gttttaccgc catcaccaaa
      241 aaaagaaacg gattccaaaa cgaaccgatg ataataagat gagccatgtg ctcgttcccg
      301 tgcggtccca caatatggta caccacccag ttcaaaatcg ccatgaggat gtagtcgata
      361 tgcgcactca aaaggtgttt ttggttttta ataattttag gaaaaagccc caagtattta
      421 acgcccacca aaagccatgc cagcgcaagc gacaccaaca agagcaccgc acccgatttt
      481 aacaaaattt ccataattac gtttccgctg gtttaacttg tggcggcaat ggcaatttga
      541 acggcttcat tggcgcctta cctttctcga cataagtgcc catgcctgca ggtacctcca
      601 cggtgacttt ttgcgcagag acagcgacct gaccctcgtg acaagcaagg cctgtgtcgt
      661 tttctgtcga agtcagttca aaaattgtac cacgtaccga cgccaccgct accggtgtac
      721 gtatcgccaa ccggccacca agactgtgca tatttcccgc ctgcgcttgc aaacggccat
      781 tttgcagagt gaatatcagc tctttattgc ttttaccgac tttatttaca ataacaaacg
      841 attggggttg aatgcgtaaa atcgcttggt tggtgagttg caagtgtacc agcgaatgcg
      901 cgccggtctt cacttggtct tcgggataaa gtcccagttt taacaaaact tccttccaca
      961 ctcgctcttg tgcgcgcgtg tattgtgcgc ttcccgattt atatatcaca accgctgcag
     1021 gttttttact caagcttgca ggcaccttga gtttcatccc gggttgaatg aggtttgggt
     1081 tggcgatttt attgtatttc aacaacgccg gccactggct ggggtcttcg agatattttt
     1141 ttgaaatttt cgccagcgta tcgccgcgtt tcacttcgac aatttgcaac tgttcttctt
     1201 cacttgtttt atccgtgctc gtcttcgtcg ttttttctgc tgcgagaaaa gaaaccgcaa
     1261 aaagaaatac tattaaaaat ttcaatttca tacgcatgat acctattctt ttatagacgc
     1321 gcagtcacgc aaaataacga cgaaaaaaat gacaacgact ccgactaccc cacggctgtt
     1381 tttacaaagt gttgcctttg cgctttttgc cgccattatt tattttttaa tcaatcgctt
     1441 cactggcgat aaacccggtg tttatctggt cacttttttt gaccacagta tccctttcac
     1501 cccttcggca gtgtttgggt acgcattagg atttactttt atctttatac cagttttatt
     1561 ttttcaacac agcgcttttt taaagaaagt attgtgggga ttcatcctgt gtacggcgtt
     1621 agccgcagtg ctgtattttg ttttccccag tcgcttaccc cgtcacggcg tgcccacgca
     1681 agaaaaattt ttttactggt gcgtggcgct catctacact ctcgatagac cagttgctgc
     1741 aatgccgagc cttttggcgc tgtacggttt tttgagtgca tggacgattt tgccgctttc
     1801 acgccgcatc ggttggggga ttcttgccgc gggcgtcatt ttttcagctg ctgcagttgc
     1861 gttgcggcaa cagttaaccg cccacatact attggcggcg cttgtcgctt atgccgtatt
     1921 cttggggatt atcaaaccga ctcttgctat aaataaaaaa gcaattaaag aaagtgcagt
     1981 accgcttttg accgtcggta aacggctcgc actattttac ggtataatta tccttgctgc
     2041 ctatctactt tttattatag gcgtgcgatt tcctctggcg ctacccgaaa attaaaagca
     2101 acgatgaaac aaaaaacaca aacacgcaac cctgttggtt ggggctatat cgaagaagcg
     2161 atcactccca gagaaaaaga agccgtctta aaaaattttc acattctttt tcccaaaggt
     2221 caattcgtgc aaaacacacc acttactgca aatcaaatca cactgacgcc tacgcgcatc
     2281 gaagtgaatc ccactttggc gcatattgtt tctatagcgc aggaaagccg cttgttacac
     2341 gcggtgggcc gcagctttcg cgacttggca aaattgcgcg aagtacaccc acttaaagcc
     2401 tccgacgcgg tggcaacgcc cacaacacaa gaagatttgt tacgcgtgct cgaatgggca
     2461 tcccaaaaga attttgcggt gattcctttc ggtggcggtt cgagcgtcgt gggtggcgtg
     2521 aatcccgaag ggatggatac atttaacggc gtgattaccg tcgacttaca atcgatgaac
     2581 cgtgtgctcg atgtcaaccg aaccgagctc gtggtgcacg ccgaagcggg gattttaggc
     2641 cccgctctcg atgctgcatt aaaactgcac aaaatgcaca cccgctattc gccgcagtcg
     2701 ttccaccaca gcacactggg cgggtggatt gccactcgcg gcgcaggtca caacgccacc
     2761 gtgtatcaaa agattgaaac gcgcgtacaa tcggtcgaag gtgttttggc caatggacgc
     2821 acttttacga cgcgaccgct ttctgcaacg agtgtatcga ttgacccgca aagctattgg
     2881 acgggcagcg aaggtatgct tggttttatt accgctgcac ggttgcgcgt gtaccctttg
     2941 ccagaacacc gtgccttccg tgcgtttgca ttccctacct ttgcgcatgc actggatacc
     3001 gcgcgccaca ttgcacaatc cgaacttttt ccggtacaag tgcgtgtcct cgatgaagat
     3061 gaaaacgcgc aaaccgcacg cttagcgggc gtcgaggcga cgggggaagc tctcctcatt
     3121 ttaggcgacg aatcgacact accaattgtc gagatgcgcc tcgatgccgt cggtaaaatt
     3181 gcgcttgagt gcgccggcaa aaaaagcgaa ggcgcgtctc ggctcttacg tgagtgggtc
     3241 cggatgtttt tccgccaacc atatttacgc gacatgctca tcgatcacaa cgtcatcgtc
     3301 gatacattcg agacatcgat cacatgggcg aaggcgctgg aattccacag cagagtgaaa
     3361 gcagtagcaa aggaagcgct catccacgaa tgcggcgccg gccatgtcgg ttgccgcgta
     3421 acgcatattt atagcgacgg attatgcctt tactattcgt tttatggttt gggaaaacgc
     3481 ggcgctctca ccaaagcctg gcaacgcatt aagcaccgcg tcagcgccga agtgattgcc
     3541 gcaggcggca cgataagtca tcaccacgcg atggggcgtg accaccgcgc gtatgcgcac
     3601 gatgaattct cgaaagcaca ccgcagcgca ctccacgacc tgaaaaaatc gctcgaccca
     3661 cagggaatta tgaacccggg actgtgtgtc agtccaccac cggcggggga ataatacgct
     3721 caggctcagc ccaactgttg agtttttcaa cccgcgacca tttctccgcc acttgcacgc
     3781 ggctaaaatc ttcggtgaac accgcttcgt tgtacgcgag cgtggcatca tcggggtaga
     3841 gagagcgggc acgcgcaaaa atttccgacg cttcgtcgta gcgcgtcatc ttgtgcgcat
     3901 aaatccccgc ttcatttaag agcgctctgt cgctcgggta tttttccaga agcgatgcaa
     3961 tctccgccca tgcgtcgtgt agcatgccgc tcataaaaag cgcacgcgcg taaaaaaggc
     4021 ggaaatttcg tgaatgccga aaaataccta agaaagcaaa gcagcgtacg ccgtcgctat
     4081 agcgctcaaa cagacaaaaa tatttgaaat aattcatgcg ctcgataagt ggaggtagtt
     4141 taccttcctc aaaaagccac gcgcgggtgt tcgtcgcgct caacactgca tcgatgcgtt
     4201 ctgccaacga ctccggcaaa tcttctttag caaacaccgc cgggttggca atatggaaaa
     4261 gcgcgtcgtg cggcgtttcg cacgcaatct ctgaaaccca tttagccaag cgcgcggcgt
     4321 taccc
//
LOCUS       DAOMPD010000207         4323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8472_length_4323_cov_2.645736, whole genome shotgun sequence.
ACCESSION   DAOMPD010000207 DAOMPD010000000
VERSION     DAOMPD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_4323_cov_2.645736"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLY93_08085"
     CDS             <1..769
                     /locus_tag="PLY93_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HRP69474.1"
                     /translation="DIPKQRCITRDNVEVDVDGVVYLKVTDAQKASYGISNYHAAVIS
                     LAQTTMRSEIGKLNLDDTFRERDKINDKVVHEIDKASEPWGIKFIRYEIRSIDPSANM
                     LNTMEKQMEAERQKRADITLAQGDKQARINVSEGEKRAAINISEGEKQKRINEAEGRS
                     REITLIADASALGLKRIAEAIQKPGGANAVKMRIVEQFLEEFGGVLAKSKLSVVPGRI
                     AELQSYFQGLSTLTENVDSRNAMPDIKAEANRKGAKA"
     gene            766..1680
                     /locus_tag="PLY93_08090"
     CDS             766..1680
                     /locus_tag="PLY93_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HRP69475.1"
                     /translation="MNVLNLIVWGGLLIFFLYKLVTSVRVISPRQAALVERLGKYQKT
                     LLSGFHVLIPFIDKVTFIQDLKEEAIAVPPQDCFTHDNVKVEVDGVIYLEVIDPVMAS
                     YGVTDFRMASIQLAQTTIRSVMGTLELDRTFEEREVINQKIVSVLSEVGQTWGIRVHR
                     YEIKNIVPPVSVKNAMERQMTAERERRAVIARSEGDMQANINTSEGLKTELVNKSEGE
                     MQKRINEAEGRAKEIEALATATAQAIETVAAAAVLPGGKEAIALRLTEGYIGKLSGIA
                     RKDARVILPSDLTRIDEQLAALGLKIRI"
     gene            1677..2135
                     /locus_tag="PLY93_08095"
     CDS             1677..2135
                     /locus_tag="PLY93_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69476.1"
                     /translation="MKLKQRGNSKEISHAAQNLLGWSLPRVLGATYGLVSKKAASPRW
                     PEFLITAFVLKKYRGKSSLGFMILHATRGMARSRQNPKHPNADALRAEAQLREILAHL
                     IGSKPRDLHRLSGIYKGLVAESGVPIAAAAITEIITTTNKKRSRKNKVKK"
     gene            2146..2607
                     /locus_tag="PLY93_08100"
     CDS             2146..2607
                     /locus_tag="PLY93_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRP69477.1"
                     /translation="MKSWILKSEPDAFSIDMLAELPDQTTLWDGVRNYMARNHLMAMK
                     VGDRGYFYHSSCKVPAIVGEVRIVSEAEVDETQFNLASKYYDAKSTHAAPRWFCPRVK
                     FLKKYEHPLSREVLKEAGLENSQLFTSFRLSVIALTSTEAKIIEKLLKVKS"
     gene            2604..3152
                     /locus_tag="PLY93_08105"
     CDS             2604..3152
                     /locus_tag="PLY93_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69478.1"
                     /translation="MNKKFILIMLFFTLTVVGFFIFSYLFLGVFSDFKRTQNVSFNAV
                     VYAGVHEGDYNKTGKEVVETRNIFLANGLTCTPILVYYDDVQKVGKPYLKSLGGCSAE
                     KEPPTEVIKILREKNKMRYTFSLKRADQFSIYGQTAVVLRKIWPELVHLSEKGAKFKF
                     PVLQVVHDNGDSDFYVEAENNN"
     gene            complement(3149..3664)
                     /locus_tag="PLY93_08110"
     CDS             complement(3149..3664)
                     /locus_tag="PLY93_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRP69479.1"
                     /translation="MQRVKTCFWSKKFRFWGVFSCAVFFLLIEAIPKSAAFAHIGNAA
                     AKIQAEESIRKNIVRGAERYLGVPYIFGGATPKGFDCSGLIHFLYARYGFSLPRSVIG
                     MRPTLRLTRAPQKGDVIFFLNDNHNVGHVGIYIDENTFIHSPREGKAIRYEKLKHPYW
                     QKRFVEYRTVL"
     gene            3760..>4323
                     /locus_tag="PLY93_08115"
     CDS             3760..>4323
                     /locus_tag="PLY93_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69480.1"
                     /translation="MLKRIFAGLIPGIDRAIEKIPPHIRDIIQKASLAFAALLALLVI
                     IAGVNKGLRDAKPKGFQAIESNRDIFYLQEMREEYAKKRKLVEDVEVDPLEFPSRQVV
                     KEDTPFVPMGRDTINHLMGEKDEALEHDNPLRPREKSPGYLGDSMSIPQRTPDKKTID
                     EDDHLTKDKIQNKPLTLPQVALPTEKKS"
BASE COUNT         1268 a          985 c         1040 g         1030 t
ORIGIN      
        1 cgacatcccc aaacagcggt gtatcacccg cgataacgtc gaggtggatg tggatggcgt
       61 cgtgtatctc aaagtgaccg atgcgcaaaa agcaagctat ggtatctcta actaccacgc
      121 cgctgtaatt tcgctggcgc aaaccacaat gcgctctgaa atcggtaaac tcaacctcga
      181 tgacacgttc cgcgagcgcg ataaaatcaa cgataaagtt gtgcatgaaa ttgataaggc
      241 gtctgagcca tggggcatca aatttatccg ctacgaaatc cgctcgattg acccttcggc
      301 gaacatgctc aataccatgg aaaagcaaat ggaagctgag cgtcaaaagc gtgctgacat
      361 tactttagcg caaggcgaca agcaagcgcg tatcaatgtc tccgagggag aaaaacgcgc
      421 tgccattaac atttcagaag gcgaaaagca gaaacgtatc aatgaggctg aagggcgcag
      481 ccgtgaaatt acactgattg ccgatgcctc tgcactggga ttaaaacgca ttgcagaagc
      541 gattcaaaag cctggcggag cgaatgcagt caaaatgcgc attgtagagc aatttttaga
      601 agaatttggc ggtgttcttg ctaaatccaa attgtccgtg gtgccagggc gcattgccga
      661 gttacaatcg tatttccaag ggctgtcgac actcactgaa aatgtcgatt ccagaaatgc
      721 gatgccggat attaaagcag aagcgaatag aaaaggagca aaagcatgaa tgtattaaac
      781 ctaatcgttt ggggtggctt actcattttt ttcttataca aacttgtcac ttcggtacgt
      841 gtgatttctc cacgccaagc agcgcttgtc gaacgcttgg gaaaatatca aaaaactctt
      901 ttgagtggat tccatgtgtt aatccctttt atagataaag tcacgttcat ccaagacttg
      961 aaagaggaag cgattgccgt acctccacaa gattgtttta cgcacgacaa cgtcaaggtt
     1021 gaagtcgatg gcgtcattta tttagaggtg attgaccctg tgatggcaag ttatggcgtc
     1081 accgattttc ggatggcgtc catccagctt gcacaaacga cgattcgttc ggtgatgggt
     1141 acgctcgaac tcgaccgcac ctttgaagaa cgcgaagtca ttaaccaaaa aattgtgagc
     1201 gtgctgtcag aggtggggca aacatggggt attcgagtac accgctacga aattaaaaat
     1261 atcgtaccgc ctgtctcggt aaaaaatgcg atggagcgtc aaatgaccgc cgagcgcgaa
     1321 cgccgtgcgg tgattgcccg cagcgaaggc gatatgcaag cgaatatcaa cacttcagaa
     1381 ggattaaaaa ccgagctggt gaataaatcc gaaggtgaaa tgcaaaaacg gattaacgaa
     1441 gccgaaggcc gtgcaaaaga aattgaagcg ttggcgactg caaccgcaca agcgattgag
     1501 acggtggctg cggcggcggt cttgcccgga ggaaaagagg cgattgcatt gcgcctgaca
     1561 gaaggctata tcggtaagct ttcgggcatc gcacgtaaag acgcaagggt gattttaccc
     1621 tccgatttaa ctcgcatcga cgaacagtta gctgcgttag ggctaaaaat acggatatga
     1681 aattaaagca gcgtggaaac tcaaaagaga taagccacgc tgctcaaaac cttttaggct
     1741 ggtcgctccc acgtgttttg ggggcgacgt atggccttgt ttccaaaaaa gcggcctcac
     1801 cgcgctggcc tgaatttttg attacggcgt ttgtcctcaa aaaataccga ggtaagagct
     1861 cgttgggctt tatgattctg catgcaacgc ggggcatggc gcgaagtcgg caaaatccaa
     1921 aacaccccaa cgccgacgcg ttgcgtgccg aagcacagtt gcgcgaaata ctggcacacc
     1981 tcattggctc taagccgcgc gatttacacc gcctttccgg tatctacaaa ggcttggtcg
     2041 cagaaagtgg tgtgccgatt gcagcggcgg cgattacaga gattatcacc acgaccaata
     2101 aaaagcgcag tcgtaagaat aaggtaaaaa aataaatttc cgcccatgaa atcgtggata
     2161 ctcaaatcgg aacccgatgc gtttagtatc gatatgctcg ccgaactgcc agaccaaacg
     2221 acgctgtggg atggcgtacg taactacatg gcgcgcaacc acctcatggc gatgaaagtg
     2281 ggcgatagag gttattttta tcattcgagc tgcaaagtgc ctgccatcgt gggtgaggta
     2341 cgcatcgtct ccgaggcgga agtggatgaa actcaattta atttagcatc gaagtattat
     2401 gatgctaaaa gtacacacgc agcaccgcgc tggttttgtc cccgcgtaaa atttttaaaa
     2461 aaatacgagc accctttgtc gcgcgaagta ttaaaagaag cagggttgga aaactcacaa
     2521 ctctttactt cgtttcgctt gtcggtcatt gcgttgacaa gcaccgaagc taaaattatt
     2581 gaaaaacttt tgaaagtgaa atcatgaata aaaaatttat actcattatg ctctttttta
     2641 ctctcaccgt ggtgggcttt tttattttct catatctttt tttgggcgtc tttagcgatt
     2701 tcaaacgcac ccaaaatgtg agctttaacg cggtcgtgta cgctggggta cacgaagggg
     2761 actacaacaa aaccggaaaa gaagttgtcg aaacgcgcaa tatctttttg gcaaatggcc
     2821 tcacctgcac accgattctc gtttactacg acgacgtaca aaaagtgggg aaaccttact
     2881 taaagtcgtt ggggggatgc tccgctgaga aagagcctcc cacagaagta ataaaaattc
     2941 ttagagaaaa aaataaaatg cggtacactt tttctctcaa acgtgcagat caattttcca
     3001 tctatgggca aacggcggtg gtactgcgta aaatttggcc ggagcttgtc cacttgagcg
     3061 aaaaaggagc aaaattcaaa ttccccgtac tgcaagtggt gcacgacaac ggcgatagcg
     3121 atttttacgt cgaagccgag aataacaatt aaagcacggt gcggtattcc acaaaccgtt
     3181 tttgccaata cgggtgcttt agcttctcgt aacggatggc ttttccttcg cgcggcgaat
     3241 ggataaacgt gttttcatcg atatagatgc ccacatggcc gacattgtga ttatcgttca
     3301 agaaaaaaat cacatcgcct ttttgcggcg ctctcgtgag acgcaaagta ggacgcattc
     3361 caataacgct tcgggggagt gaaaaaccat accgcgcata aagaaagtgg atgagcccgc
     3421 tacaatcaaa gcctttcggt gtcgctccgc caaaaatata gggtacgcct aaataccgct
     3481 ccgccccccg cacgatattt ttgcggatac tttcctctgc ttggatttta gcggcggcgt
     3541 tgcctatatg cgcaaatgcc gcacttttgg ggattgcttc gatgagaaga aaaaagactg
     3601 cacagctaaa aacaccccag aaacggaatt tcttgctcca aaagcaagtt ttaacgcgtt
     3661 gcataggtta ttttttgtgc gccgcacaag ctgtcaaact ctttcgatac cagtaaaatc
     3721 ttcattattt tttatccaat tcatacgata ctatattata tgctaaagcg catttttgct
     3781 ggtcttatcc ctgggattga ccgcgccatt gaaaaaatac caccgcatat acgcgacatt
     3841 atccagaaag cctcgctggc gtttgccgcg ctcttagcgc ttctggtgat tatcgctggc
     3901 gtcaataaag ggttgcgtga cgccaagcca aaaggttttc aggcaattga atctaaccgc
     3961 gatatttttt atttacaaga gatgcgcgaa gaatatgcaa aaaagcgtaa gcttgtcgaa
     4021 gacgttgaag tggatccgct ggaattcccg tcgcgccaag tggtgaaaga agacactccg
     4081 tttgtgccga tggggcgcga tacaattaac catctcatgg gcgaaaaaga cgaagccttg
     4141 gagcacgaca atccgctgcg accacgcgaa aaatcccctg gctatttagg cgatagcatg
     4201 tcaatcccac aacgcacgcc agataaaaag actatcgacg aggatgacca cttaacgaaa
     4261 gataaaattc agaataaacc attaacatta ccacaagtcg ctttaccaac agagaaaaaa
     4321 agt
//
LOCUS       DAOMPD010000208         4315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8495_length_4315_cov_2.568075, whole genome shotgun sequence.
ACCESSION   DAOMPD010000208 DAOMPD010000000
VERSION     DAOMPD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_4315_cov_2.568075"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLY93_08120"
     CDS             <1..511
                     /locus_tag="PLY93_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP69481.1"
                     /translation="FRILLEEPEPSLNIALERLNSLLYKNIQGRLEDTRNMTLSLLRH
                     ENGHIDICGQHEKVILVRHDGTLEIHDTANLGIYVGLMEDISYTINLLSLTLNKGDQI
                     LLFTDGATEAENASKEQFSEKRLATAFKKYAHQSTQEIVESLKAEILAWQGDVPLMDD
                     ITLVVVRRT"
     gene            540..1106
                     /locus_tag="PLY93_08125"
     CDS             540..1106
                     /locus_tag="PLY93_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRP69482.1"
                     /translation="MAKEVILGEFNKIADTMPAESQAKFEFHPIDLMTHWKRCGLLAD
                     FIGAFYSFGNPPRIDKDENMLMNLSTVVNELIENAAKFSRQRAPVSIQLKHYSSILQI
                     EVTNQTVHTVAQNFQKIANKLLSENIEELYFKSLEERDENDNKTSGIGLMMILKDYPV
                     QMAFRFKELEDGKHTEITTKAYISVQGV"
     gene            1108..1461
                     /locus_tag="PLY93_08130"
     CDS             1108..1461
                     /locus_tag="PLY93_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69483.1"
                     /translation="MDIRENDYSVVYEEGAKTVKFQGNIRLQGVSEYEPIKSLLGACA
                     EQLTGSDTLTLDFQGLKFLNSAGITTLSMFVIAMRKKNTTKLAVVGSKVVAWQEKSLN
                     NLNKIWSDIQVSFHE"
     gene            1454..2581
                     /locus_tag="PLY93_08135"
     CDS             1454..2581
                     /locus_tag="PLY93_08135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP69484.1"
                     /translation="MNETELKPIGKVELAQSADDLDAVFRFRYEVYTEEMGFHFPWSD
                     DAARRLKDRVDAQSLNFFIRNSEGEIVGVIRLHTFSDQNVTKTLAEKYETHHFSETYE
                     GSSRMLIARFLIRKDYRARSKAAWKLILEAYRQCELNNAPLVFIDCSPHLVKFYEALG
                     FRRYAANFTDEVLGYKIPMVILLNDTPYLEKVSSPLFLLARHRADARSMEAWYNAQFP
                     KFGNSVFLQQFDSQELLANVASHAGTLPENFHKVSQKLKSSCLIHVKKGDTVLKQGEM
                     SNEMYLVLKGTLDVLFSNDGKNKQSVTILGPGEVFGELGFLMRSPRTSTVVALEDAEL
                     LMIAADKAEQLIEIHPKESAVLFLDLIRLLAQRYVERYQTV"
     gene            2591..3313
                     /locus_tag="PLY93_08140"
     CDS             2591..3313
                     /locus_tag="PLY93_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69485.1"
                     /translation="MRKLQYITLCFFVGAGHLFAESIQPVPDGNIVYAGAGVDGAVVL
                     EGGYVTPQLFSLLPLDISLAIPTGKIFNTPGSYRLGLHTHWTRRFYNNWGFNLTPGTR
                     IYTLRTPAWSGFAWHLDFQGQVGYIAEKWAATLDLGLSPKLTTYVGINELVTQNFPEA
                     KSGFYAKGFEAMLHYGVSGYWRFTDHFAAGLRLWLTLPIEDSKSPLARTVTDGVISFN
                     VTYYFDAIQKKSEKGSNEGGVQ"
     gene            3313..>4315
                     /locus_tag="PLY93_08145"
     CDS             3313..>4315
                     /locus_tag="PLY93_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP69486.1"
                     /translation="MERFILAKRFARRAFARKAVSLVFAAFFFTACGNDPSQDGFLVP
                     KTVKDDTSLPSEMVRGTKLHLMIETKGAPGPGDNIIFLHGGPGYDYRSLILLKDKVKA
                     SLTNAKIVMYDQRGGGLSERLDPSDSSLSVASHVEDLEAIRVTKFNAPMKVIAHSFGG
                     ALLQAYIAKYPANVTNAVFISSMPPKNNKDDDLLESIFNTAAGDYTWQRNLLGESHAE
                     LDFRFINFLSAQPYYYCSENDYKKVPFWRFGYVATIKTMENGLLASPVSGPSVTYGYD
                     FVSTNAAYTGKSAFIVGACDTKLGKPVANALSATLGGTKRAVVEVSDAGHFTFTDQLN
                     "
BASE COUNT         1161 a          968 c         1066 g         1120 t
ORIGIN      
        1 gttccgtatt ttacttgaag aacccgaacc gtcgctgaac atcgcactcg aaagactcaa
       61 cagccttctt tacaaaaata tccaagggcg tcttgaagac acgcgcaaca tgacgctctc
      121 tttgttgcgt catgaaaatg ggcatatcga tatttgtggt cagcacgaaa aagtgattct
      181 tgtgcgtcac gacggtactc ttgagataca cgacaccgcc aatttgggaa tttatgtggg
      241 tctcatggaa gatatttcgt ataccatcaa tttacttagt ttaacgctca acaaaggcga
      301 tcaaatttta ctttttaccg acggagctac cgaggcagaa aacgcaagta aagaacaatt
      361 ttcagaaaaa cgtttggcca ctgcgttcaa aaagtacgca catcaatcga cgcaagaaat
      421 tgtagagtcg ttgaaagcgg aaattttagc atggcagggg gatgtccctc ttatggatga
      481 tattacgctg gtggtcgtgc gccgcacata actaatttaa ttttttggaa ggagagtata
      541 tggctaaaga agtaatttta ggtgaattta acaaaattgc cgacacgatg cccgctgaaa
      601 gccaggcgaa gtttgagttc cacccgattg atttgatgac gcactggaag cggtgtgggt
      661 tactcgccga cttcatcggc gcgttttatt cgtttggtaa tcctccgcgc atcgacaaag
      721 acgagaatat gttgatgaac ctttcaacgg ttgtgaacga actgattgaa aatgccgcca
      781 aatttagtcg gcagcgcgcg ccggtgtcga ttcaactgaa acactattcc agtattttgc
      841 aaatcgaagt gacaaatcaa accgtccata cggtggcgca gaattttcaa aaaatagcga
      901 ataagctcct ctctgaaaat atcgaagagt tatattttaa gagtctcgaa gaacgggacg
      961 aaaatgataa taagacatca ggtattgggc tcatgatgat tctgaaagat tatccggtac
     1021 aaatggcatt ccgcttcaaa gaactcgaag acggtaagca caccgagatt acgacaaagg
     1081 cctatatttc ggtacaaggg gtataaaatg gatattcgcg aaaatgatta tagcgtcgtg
     1141 tatgaagaag gcgcaaagac agtaaaattc caagggaaca ttcgcctaca aggcgttagc
     1201 gaatacgagc ctataaaaag cttactgggt gcctgcgccg aacagcttac cggaagcgac
     1261 acgctgacgt tagattttca agggcttaaa ttcttgaata gtgctgggat taccacactg
     1321 agtatgtttg tgattgcgat gcgtaaaaaa aacacaacga aactggcggt tgtgggtagc
     1381 aaagtggtgg cgtggcaaga gaaatcactc aataacttga ataaaatctg gagcgatatc
     1441 caggtctcat tccatgaatg agactgaatt aaaaccgata ggtaaagtcg agcttgcaca
     1501 aagcgcagac gatctcgacg ctgtatttcg atttcggtac gaagtttaca ccgaagagat
     1561 ggggtttcat ttcccgtggt cggacgatgc cgcgcgtcgg ttaaaagatc gtgtcgacgc
     1621 tcaaagcctc aacttcttta ttcgcaacag cgaaggtgaa atcgtcggcg ttatccgtct
     1681 gcacactttt agcgatcaaa atgtgactaa aactctggcg gaaaaatacg aaacgcacca
     1741 tttctccgag acttacgagg ggtcgtcgcg gatgctcatc gcacgctttt tgatccgcaa
     1801 ggattaccgc gcgcgcagta aagccgcatg gaagcttatt ttagaggcgt accgtcaatg
     1861 cgaactcaat aatgcgccgc ttgtgtttat cgattgttct ccgcatctgg tgaaatttta
     1921 tgaggcgttg ggctttcggc gttacgcggc aaatttcacc gacgaggtgt tggggtataa
     1981 aataccgatg gtgattttac tcaacgacac tccttatctt gaaaaagttt cttccccgct
     2041 ttttctttta gcgcgccacc gtgcggacgc acgcagcatg gaagcatggt ataatgcgca
     2101 gtttccgaaa ttcggtaatt cagttttttt gcaacagttt gactcgcaag aattgttagc
     2161 caatgtcgcc tcgcacgcgg ggacactccc cgaaaacttc cataaagttt cgcaaaaact
     2221 caagtcgagc tgtctcattc atgtcaaaaa aggcgatacc gtgcttaagc aaggcgaaat
     2281 gagcaacgag atgtacttgg ttttgaaagg cactctcgac gtgttgtttt caaacgacgg
     2341 caaaaataag cagagcgtta ctattttagg gccgggtgaa gtttttggcg aattgggttt
     2401 tttaatgcgt agtccacgaa catccacagt ggtagcgtta gaagatgcgg agctactcat
     2461 gattgccgcc gacaaagcag aacaactcat tgagattcac cccaaagaat cggcagtttt
     2521 atttttagat ttaattcgtc ttttagcgca acgctatgtc gaacgatacc aaacagtata
     2581 ggtggtcagc atgagaaaat tacaatacat aacactttgt ttttttgtgg gtgccggaca
     2641 cctttttgcc gaaagtattc agcctgttcc cgatggcaac attgtatacg caggggcggg
     2701 tgtcgacggt gcagtcgttc tcgagggcgg ctatgtaacg ccccaattat tttcactctt
     2761 gcctcttgat atctcattgg caatacccac cggaaaaatt ttcaataccc cgggttcgta
     2821 tcgtttggga ttacataccc actggacgcg ccgcttttac aacaactggg gttttaatct
     2881 caccccaggc acccgtattt atacgctgcg cactccggcg tggtcggggt ttgcgtggca
     2941 tctcgatttt caggggcagg tgggatatat cgccgaaaaa tgggcggcga cgttggactt
     3001 ggggctctca cctaaactta ccacctacgt ggggataaac gagcttgtta cacaaaattt
     3061 ccccgaagca aagtcgggtt tttacgcgaa aggctttgag gcgatgctgc actatggcgt
     3121 ttcaggttat tggcgcttta ccgaccattt cgccgccggt ttgcgcctgt ggctcacact
     3181 gccgattgaa gacagcaaga gtcctctcgc gcgcactgtc accgatgggg ttatctcgtt
     3241 taacgtgacg tattatttcg atgcaattca aaaaaaatcc gaaaagggga gtaatgaagg
     3301 gggagtgcaa taatggaacg ttttatttta gctaaaagat ttgctcgtcg tgcgttcgca
     3361 cgcaaggcag tgtcgttggt atttgctgca ttttttttta ccgcgtgcgg caacgaccca
     3421 tcgcaagacg gctttctggt gcccaagacg gttaaagacg acacgtcgct gccgtcggaa
     3481 atggtacgtg gcacaaaact gcacttgatg atagaaacta aaggcgcgcc aggtccgggg
     3541 gataacatta tttttttgca cggcggcccg gggtatgact accgctcgct tatcctgttg
     3601 aaagataaag tgaaagcatc gctcacaaac gctaaaatcg tcatgtatga ccagcgcggt
     3661 ggtgggcttt cggagcgatt agacccttcg gattcttcgc tttcggtggc aagccacgtc
     3721 gaggatttag aagctatccg cgttacgaaa tttaatgccc ccatgaaagt tattgctcac
     3781 tcattcggtg gcgcgttgct tcaagcgtat atcgccaaat accccgctaa cgtcaccaac
     3841 gcggttttta tttcgtcgat gccgcccaag aacaacaaag acgacgattt gctcgaatcg
     3901 attttcaata ccgctgcggg ggactacacc tggcagcgca atctcttggg agaaagccac
     3961 gctgagcttg attttcgttt catcaatttt ctctctgcgc agccgtacta ctattgtagc
     4021 gagaacgatt acaaaaaagt accgttttgg cgttttggtt atgtcgccac catcaaaacc
     4081 atggaaaacg gtcttttagc aagtcctgtt tcgggaccgt cggtgacgta cggctatgat
     4141 tttgtatcga ccaacgctgc gtacacgggc aagtcggcgt tcattgtggg cgcgtgcgac
     4201 accaagttgg gaaagccagt ggccaatgcg ctcagcgcca cattgggcgg gacaaaacgt
     4261 gcggttgtgg aagtctcgga tgcagggcac tttaccttca ccgaccagct taacg
//
LOCUS       DAOMPD010000209         4305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8512_length_4305_cov_2.728706, whole genome shotgun sequence.
ACCESSION   DAOMPD010000209 DAOMPD010000000
VERSION     DAOMPD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8512_length_4305_cov_2.728706"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLY93_08150"
     CDS             complement(<1..329)
                     /locus_tag="PLY93_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69487.1"
                     /translation="MLAAGLGCYYDANPPDESLIHDEKPLLMLDINLGAGVTQKSIQI
                     KFTDAFGSTTLEGASLKTDATGRLLYPLRMQHGTRGYTLDSIIVDQSGQGTFAEAADL
                     TWDKIASP"
     gene            complement(358..2001)
                     /locus_tag="PLY93_08155"
     CDS             complement(358..2001)
                     /locus_tag="PLY93_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP69488.1"
                     /translation="MIQPNVFTGENPGLKPFDLSTYTGTKNKNFFTETPILFEAFELN
                     ATNTDRTYLEDVKKHLSGLGHLLGGVVDELTVACHREGKWGELVQFDKFGNRIDEIRY
                     SSEQRELRKIFYDYGVVNLDCHEDWKHEFSTAHRMALATLSNMNGEGGVSCPFAMTEG
                     IVHAIRALGTDEQKERYLPMLTDPASSSHLMAGQYVTERVGGSNVAANRTIATEQKDG
                     TWLLNGEKWYCSNPGDLWVTTAKIQGTNVVGLFLVARLKRDGSLNGCRLLRKKEILGS
                     KGKLTVESVYENCEATALGRPSHGLANLIRYILRTSRLHVVCGGLGHIARGILEAEAY
                     VSQREAYGAKLVDFPSVRRTLDEIKMLQTALTLVSFRAFRFAADDDTLELLMLPLMKV
                     VATQYSAMIVKEAIILHGGNGILEDFSVLPRVLNDAIINETWEGTHALLAENALRALK
                     RPKVRASMGAYFEKEKKILPQEVLTLAETFLADIDAMMASEHDRDVNRMLLCEKLWRF
                     FALIEMERWVKKYPSAAHTAQAKLLLTFVNQNARMPYKI"
     gene            complement(2007..2936)
                     /locus_tag="PLY93_08160"
     CDS             complement(2007..2936)
                     /locus_tag="PLY93_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRP69489.1"
                     /translation="MSSHPSIYPLLPYPVLKPWGGGHLNALTQKLNGENVGEYVIFSN
                     LPQFPVQVRTPKGNFPLADFWPRVFSNEELPFMIKILSTAEPLSLQNHPSDDDVKELG
                     LPGKGKFECWTVLDAADDASAYLGIKEGFWPSTLYALDKEENPLRFFNNYALKKGDVV
                     RLDPGLIHTTTGRLLFYEVQQVSDHTFRIYDFGRGRALHLKEAVHCVRDQKPSITSWT
                     SALETPYFSVAYHACNKSLTLTRRGEKISVFTWLGRDAELTTAEGKLYLRWGDSFLAA
                     GAKKTALQIKVGIRVNPTLEKLPLLDMLFEAYV"
     gene            complement(3308..3739)
                     /locus_tag="PLY93_08165"
     CDS             complement(3308..3739)
                     /locus_tag="PLY93_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRP69490.1"
                     /translation="MRAICDKKNILAVSDALQNVEILCGDFEQTLPYAESSNTLFYFD
                     PPYKPLNITSNFNSYGKDEFTDQEQIRLRNFCSKIDALGYSWILSNSDVKEVDTNNDF
                     FDTLYSNYPIQRVLAKRSINSNPEKRRPLTELLITSHAVSL"
     gene            complement(3749..>4305)
                     /locus_tag="PLY93_08170"
     CDS             complement(3749..>4305)
                     /locus_tag="PLY93_08170"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HRP69491.1"
                     /translation="FAATIGSEMTAAAAGTIGKVRRDPFAMIPFCGYHMADYFNHWLQ
                     VGRDLGNPPRMFFVNWFRKDQDGKFIWPGFGENTRVLEWIVDRVHGKGSAIESPLGWM
                     PRYDEMDWEGLDFPREKFNKLMTINTRLYKDEVLQHEEFFDRIYDKLPKELFFIRQLI
                     LSSLWRTGDDWVVGQVGVTTHSTK"
BASE COUNT         1087 a         1056 c          994 g         1168 t
ORIGIN      
        1 ggagatgcta tcttatccca cgttaaatct gcggcctctg cgaaagtacc ctgtccactc
       61 tggtctacga ttatagaatc caacgtatag ccccgcgtgc cgtgttgcat tcgcagcgga
      121 tacaagagtc tccccgtcgc atcggttttg agcgacgccc cctcaagcgt tgtgctgccg
      181 aacgcatcgg taaactttat ttgtatgctc ttttgtgtca ctccagcgcc gaggttgatg
      241 tcgagcatca acagaggttt ttcatcgtgg atgaggctct cgtcgggtgg gttggcgtca
      301 tagtaacaac caagccccgc tgcgagcaaa actgcacaaa gcagttttat cctccattta
      361 gattttatac ggcatccgtg cattttgatt aacaaatgtt aaaagaagtt tggcttgcgc
      421 ggtgtgtgca gcgctcggat atttttttac ccaacgctcc atttcgatga gcgcaaaaaa
      481 ccgccacagt ttttcgcaaa ggagcatccg gttgacatcg cggtcgtgct cggatgccat
      541 catcgcgtcg atatcggcaa ggaatgtctc agcaagagtg aggacttctt gcggtagtat
      601 ttttttctct ttctcgaaat atgcccccat actcgcgcgc actttggggc gcttcaacgc
      661 ccgcaacgcg ttctcggcca agagcgcgtg cgtgccttcc cacgtttcgt tgataatggc
      721 gtcgttcaat acgcgcggta acacgctaaa atcttccaga ataccgttgc caccgtgcag
      781 aatgattgct tctttaacga tcatcgcgct atactgcgtc gccaccactt tcatgagcgg
      841 tagcatgagt aactccaacg tgtcgtcgtc ggcagcaaag cggaaagccc taaaagaaac
      901 gagtgtgagc gctgtttgca gcatttttat ttcgtctaaa gtgcgcctga cgctcggaaa
      961 atccaccaac tttgcgccgt aggcttcacg ctgcgaaacg tacgcttcgg cttctaaaat
     1021 accgcgtgcg atatgtccca atccaccgca caccacgtgc aagcgcgatg tgcgtaaaat
     1081 ataccggatg aggttggcga gtccatgcga agggcgaccc aacgcggtgg cttcgcaatt
     1141 ttcataaacg ctctccacag tgagcttgcc tttagagccg aggatttctt tcttacgtaa
     1201 aagtcggcag ccgttgaggg agccatcccg tttgaggcgt gcgactaaaa aaagtccaac
     1261 gacgttggtg ccttgaattt tggcggtcgt cacccacaag tcgcctgggt tggaacaata
     1321 ccacttttcc ccattcaaaa gccaagtgcc gtctttctgt tcggtggcaa tcgtgcggtt
     1381 ggcggcgacg ttggaaccgc cgacacgttc ggtcacgtat tggcctgcca tgaggtgcga
     1441 tgaagacgca gggtcggtga gcatcggcaa gtagcgttct ttctgttcgt cggtgccgag
     1501 cgcgcgaata gcatggacga taccctcggt catcgcaaaa gggcacgaca ccccgccttc
     1561 accgttcatg ttcgagagcg tggcgagcgc catgcggtgc gcggtggaaa attcgtgttt
     1621 ccaatcttcg tggcaatcga gattgacaac gccataatcg taaaaaattt ttctgagctc
     1681 acgctgctca ctcgaatagc ggatctcatc gatgcggttg ccgaatttat cgaactgcac
     1741 caattcgccc catttgccct cgcggtggca tgcgacggtg agttcgtcca ccacaccgcc
     1801 taaaagatgc cccaaccccg agagatgctt tttgacatct tctaaatacg ttctgtcggt
     1861 gttcgtggcg ttgagttcaa acgcttcaaa gagaatggga gtttcggtaa aaaaattttt
     1921 gttttttgta ccggtgtacg tgcttaaatc aaacggttta agacccgggt tttcgcccgt
     1981 aaaaacattc ggttggatca taccactcac acgtacgctt caaacagcat gtcaagtaat
     2041 ggcagttttt ccaaagtggg atttaccctg atgccgactt taatttgtag tgcggttttt
     2101 tttgcacccg ccgctaaaaa gctatccccc cagcggagat acaattttcc ctctgctgtc
     2161 gtcagctctg cgtcgcgacc caaccaagtg aacaccgaaa ttttctcacc gcgccgcgtg
     2221 agtgtgagag acttattgca cgcgtggtag gcaaccgaga aataaggagt ttcgagcgcg
     2281 ctcgtccatg aagttatcga cggtttttga tcgcgcacgc agtggacggc ttctttaaga
     2341 tgcaaagcgc gcccccgccc aaaatcataa atacggaatg tgtggtcgga gacttgctgc
     2401 acttcataaa acaacaaccg accggtggtg gtgtggataa gccccggatc caaacgcacc
     2461 acatcgcctt ttttaagtgc ataattattg aaaaagcgca aggggttttc ttctttatct
     2521 aacgcataaa gcgtacttgg ccaaaatccc tctttaatgc ccaaataagc actggcgtcg
     2581 tcggcggcgt ccaagaccgt ccaacattca aacttgcctt tgccggggag tcccaattct
     2641 ttcacgtcgt cgtcggaggg gtggttttgc aacgagagcg gctcggcggt cgataaaatt
     2701 ttaatcatga agggcagctc ttcgttcgag aaaacgcgcg gccaaaaatc tgcaaggggg
     2761 aaatttccct tgggtgttct cacctgaaca ggaaattgtg gcaggttgga gaagatgaca
     2821 tactcaccga cattttcccc gttcaatttt tgcgtcagcg catttaagtg tcctcccccc
     2881 cacggcttca agacggggta tggaaggagg gggtagattg aggggtgaga gctcatttgt
     2941 ggtaaatgga attttatatc cgattcaatt atttataatc caaatttttt tacgatataa
     3001 ttctacaata tctttatttt ctatatcttg ttttcacttt atactcattt ctaaaaaatc
     3061 tttggcttga tcaattccaa caaatcgccg atttacaaga ttagcagaaa ttcctgtggt
     3121 actactccct gtaaatggat ctaagaacca agcgtaccgc tttgaagaga aaggttatta
     3181 ttgaataaaa aatatgatat atctgcaaaa cccatgtcga attttttgtc aatttttggt
     3241 aacacctcca tgacatcgcc atggtataaa ttaaaatttt gatctgacga ttgaaaataa
     3301 agcattttca taacgacact gcatggcttg ttatcaacaa ttctgtcaat ggccgtcttt
     3361 tctctggatt agaatttata ctccttttgg ctaaaactct ctgaatagga taatttgagt
     3421 aaagagtatc aaaaaaatca ttattcgtat cgacttcttt tacgtcagaa ttgcttaaaa
     3481 tccaagaata acctaacgcg tctatttttg agcaaaaatt acgcaaacga atttgttctt
     3541 gatctgtaaa ttcatccttt ccataagaat taaaatttga agttatattc aagggtttgt
     3601 atggaggatc gaaataaaat aacgtatttg aactttctgc atagggaagc gtttgttcga
     3661 aatctccaca aagaatctcg acgttttgta aggcatcgct taccgctaaa atattttttt
     3721 tatcgcaaat cgctctcaag ccgccaccct acttcgtcga gtgcgtcgtc acgcccacct
     3781 gccccacaac ccagtcgtcg ccagtgcgcc ataagctcga caagatgagt tggcggataa
     3841 agaataattc tttaggaagc ttgtcgtaaa tgcggtcgaa aaattcttcg tgttgcagca
     3901 cttcgtcttt ataaagacgg gtgttgatgg tcatgagttt gttgaatttt tcacgaggga
     3961 aatccaaccc ttcccaatcc atttcgtcgt aacgcggcat ccatcccagt gggctctcga
     4021 tggcagatcc tttgccatgg acacgatcga caatccactc caagacgcgt gtgttttcgc
     4081 caaagccagg ccatataaac ttgccgtctt ggtctttgcg aaaccagttt acaaaaaaca
     4141 tccgaggggg atttcctaag tcacgcccca cttgtaacca gtggttgaaa taatccgcca
     4201 tgtggtaacc acagaaagga atcatcgcaa aaggatcgcg gcgcacttta ccgatggtgc
     4261 ccgccgctgc agcggtcatt tccgatccga tggtcgcagc aaagt
//
LOCUS       DAOMPD010000210         4300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8525_length_4300_cov_2.098233, whole genome shotgun sequence.
ACCESSION   DAOMPD010000210 DAOMPD010000000
VERSION     DAOMPD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8525_length_4300_cov_2.098233"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..>380)
                     /locus_tag="PLY93_08175"
     CDS             complement(91..>380)
                     /locus_tag="PLY93_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRP69492.1"
                     /translation="YYDNATLHIYLASGKGEYESLRKRHVFYEANVLHRNSLKGWKWI
                     SDIFAVALILIALTGLLIPQGKNGALRGGEWYMAAGLLLPTGAVALFYLLT"
     assembly_gap    381..402
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<403..675)
                     /locus_tag="PLY93_08180"
     CDS             complement(<403..675)
                     /locus_tag="PLY93_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69493.1"
                     /translation="MSEGKSVKQKITKASRVIHRDLGYFFLGLIVAYSISGLALNHVD
                     DWNPDFVIAKKNITVQPQAAGYKADEATLLSYNALVGEEKHKTVDYP"
     gene            complement(706..>1383)
                     /locus_tag="PLY93_08185"
     CDS             complement(706..>1383)
                     /locus_tag="PLY93_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRP69494.1"
                     /translation="EYTEPSIATRMKEFDVEGFTDVVVVPIFLTVSPHPFDDIPTILG
                     QKNNPESREAMRLEKIERYTPKAKVIITPLLDFTDILEKNLIRRVNALSKDKSKEALV
                     MIAYGDEKYENEWNKLCDKMGASVRKNLGIQDYSYGWCGHLVHYNPLKTTEAIEKVAK
                     KGKRALVVPILVAVDEMFQGKIIPQGVEKAKKAGYDVVYKPDSILPDKGLEDWVVKVS
                     RQYVQKL"
     assembly_gap    1384..1393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1394..1616)
                     /locus_tag="PLY93_08190"
     CDS             complement(<1394..1616)
                     /locus_tag="PLY93_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HRP69495.1"
                     /translation="MKTNIRFLISLFALAAVVVTPTASKQTKVKKKVGLLLVNHGSRS
                     AAWRASLMQLEKNVRSEILAEKHFTDVRTA"
     gene            1864..3588
                     /gene="leuA"
                     /locus_tag="PLY93_08195"
     CDS             1864..3588
                     /gene="leuA"
                     /locus_tag="PLY93_08195"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRP69496.1"
                     /translation="MKPEIVEKYKPYRPLDLKDRTWPNKSITKAPRWCSVDLRDGNQA
                     LVFPMEIPAKSELFKLLVEIGFKEIEVGFPSAADVEYNFLRHLVDKKLVPPDVTIQIL
                     TQAREHLIRKSFDALAGVHRAIVHMYNPTSTLQRDVVFGKSRDEIKKLAVDGIRLVKQ
                     LAVEYHKKTGTIFELEYSPESFTGTEGDFAVEVCAAVMDVWEPTEDNKIILNLPSTVE
                     MTTPNIYADQIEYVIRNLPNRDLAVISLHTHNDRGCGVAAAELALMAGADRVEGTLFG
                     NGERTGNCDLVTVALNMFTQGVNPHLDFSNINRVAEVYERTTRMEIHDRHPYVGELVF
                     TAFSGSHQDAINKGLHRIAVQKDKVEWAVPYLPIDPSDIGRTYDSIIRINSQSGKGGV
                     AYVMEHDFGCKLPKTMHPEFADVVQAIAEKQGGEVIPSEINEAFKNEYLDRKEPLELL
                     GISSIHENPDTGDVKLELKMKSNFNQEGFLSSSETNFAGEGNGPLDAARNAIQRATDN
                     KISFQIKNYSEHSLGEGSDARAIAYIQIECTDKKTFYGVGISPNISRASIEALVSAVN
                     RAWQSIRN"
     gene            3674..>4300
                     /locus_tag="PLY93_08200"
     CDS             3674..>4300
                     /locus_tag="PLY93_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRP69497.1"
                     /translation="MVAEHPEYKPGLDGVIACITKLSWLDTEKEQIVVRGYDLIELIQ
                     KTSYIKVAYLLIYNKFPTDAEAKEFEQKLMAQADIPEEMYQIFKLLPKNTMAMDALRT
                     GISILGGYEEHAALMDTSTKSNEEKALKSLAKAPAIAVNSYRAITGQPFVKPDKSLGY
                     SENFVYMIKGSKPTAVETDTLDKILTTYIEHEMPNSTFAVRVVSSTLSD"
BASE COUNT         1130 a         1003 c         1022 g         1113 t
ORIGIN      
        1 atgagaaaaa ttgccacgaa gtgcagttga tagccgcgtc tattttgatt tttcctttcc
       61 atagttatcc tccgttttct ctattttact ttacgtgagt aaatagaaga gcgccacagc
      121 acctgtcggc aaaagtaacc ccgcagccat gtaccattcc ccaccccgca gcgccccatt
      181 tttcccttgt gggattaaaa gaccggtcag tgcaatgaga atgagcgcca ctgcaaaaat
      241 atctgaaatc catttccagc ctttaagaga gttgcggtgt agcacgttgg cttcataaaa
      301 gacgtggcgt ttacgtagac tttcgtactc ccccttgccg gaggcaagat agatgtgtag
      361 tgtggcgttg tcgtagtaga nnnnnnnnnn nnnnnnnnnn nnggggtaat ctacagtctt
      421 gtgtttttct tcccctacaa gagcgttgta agacagcaag gtggcttcgt ccgctttata
      481 gcctgctgct tgaggctgta cggttatatt tttcttggcg atgacgaaat cggggttcca
      541 atcgtcgacg tggtttagcg caagccctga aatcgaatac gcgacgatga gccccaaaaa
      601 aaagtaaccc aagtcgcggt ggatgactcg cgacgctttg gttatttttt gttttacgct
      661 tttaccttca gacataaatc tctcttaaaa tagtgaatac gatggttata gtttttgcac
      721 gtactggcgt gaaactttta ccacccagtc ttcgaggcct ttgtcgggta aaatagagtc
      781 gggtttatag acgacatcgt agcctgcctt ttttgctttt tcgacgcctt gggggatgat
      841 tttaccttgg aacatttcat ccacagcgac gagtatcggc acaaccaatg cgcgtttgcc
      901 tttcttggct actttttcaa tcgcctcggt agttttcaat gggttatagt ggactaagtg
      961 accgcaccaa ccatatgaat aatcctgaat ccccaaattt ttacgcacgc tggcgcccat
     1021 tttgtcgcaa agtttattcc attcgttttc atatttttca tcgccgtacg caatcatcac
     1081 caacgcttct ttagacttat ctttagagag cgcattaacg cggcggatta agtttttttc
     1141 gagtatgtca gtaaaatcaa ggagaggggt gatgatcact tttgctttgg gggtgtagcg
     1201 ctcaatcttt tcaaggcgca ttgcttcgcg cgattcggga ttattttttt ggcctaaaat
     1261 ggtgggtatg tcgtcgaaag ggtgcgggct caccgttaaa aaaatgggta caacgacgac
     1321 gtcggtaaaa ccttcgacgt caaattcttt catacgcgtg gcgatcgacg gttcggtgta
     1381 ttcnnnnnnn nnnacgcggt acgtacgtct gtgaaatgct tttcggcaag tatctcggag
     1441 cgtacatttt tttcaagctg catgagcgaa gcgcgccatg cagccgagcg tgagccgtgg
     1501 tttacgagaa gcaagccgac tttctttttt actttggttt gttttgaagc tgtgggtgta
     1561 actaccactg ctgcaagcgc aaaaagggag attaaaaatc gtatatttgt tttcatagaa
     1621 gatgagaatc attctcagta tcattcatgg tgtaaagtat ttttataatt ttatggacgc
     1681 cgcgccgata tttagtatat taacaacatg ccgatggcca acgccacttc gagtaaattt
     1741 ctttctttaa tcggctcact attcctattg cttcgactat aatgcgccat aagggcaaat
     1801 tcgtgcacaa cgcacacctt atggcagccg cgcaaaagcg catcattctt ttttggaggc
     1861 aatatgaaac ctgaaatcgt tgaaaaatat aaaccctacc ggcctttaga cttaaaagat
     1921 cgcacttggc cgaataagtc gatcaccaaa gcgccacggt ggtgcagcgt ggatttacgc
     1981 gacggcaacc aagccttggt gtttcccatg gaaattcccg ccaaaagcga gctttttaag
     2041 ctcctggtgg aaattggctt caaagaaatt gaagtgggat tcccctcggc ggcggatgtc
     2101 gagtacaatt ttttgcggca cttggtggat aagaaactcg ttccccccga tgtcaccatc
     2161 caaattttaa cgcaagcgcg cgagcatttg atccgcaaaa gttttgacgc gttggcgggc
     2221 gtgcaccgcg cgattgtgca catgtataac cccacgtcca ccttgcagcg cgatgtggtc
     2281 tttgggaaaa gccgcgacga aattaaaaaa ctcgccgtcg acggtatccg cttggtgaag
     2341 cagttggcgg tggagtacca caaaaaaacc ggcactattt ttgaactcga atactcgccc
     2401 gaaagtttca ccggtaccga aggggatttt gctgtcgagg tgtgtgctgc ggtgatggac
     2461 gtgtgggagc ccacagaaga taataagatt attttgaact tgccaagcac tgtcgaaatg
     2521 acgacgccca acatttatgc cgaccaaatt gagtacgtca tccgcaattt acccaaccgt
     2581 gatttagccg ttatcagttt acatacgcac aacgaccgag gttgcggcgt tgctgcagcg
     2641 gagttagcgc tcatggcagg ggcagaccgt gtcgaaggga cgcttttcgg taacggcgaa
     2701 cgaaccggaa actgcgattt agtcactgtg gcgttgaata tgttcacgca aggggtaaat
     2761 ccccacctcg acttctcgaa catcaaccgt gtcgctgagg tttacgaacg caccacacgg
     2821 atggaaatcc acgatcggca tccgtacgtg ggtgaattgg ttttcaccgc attttcagga
     2881 tcgcaccaag acgcgatcaa caaaggactg caccgcatcg cagtgcaaaa agataaagtc
     2941 gagtgggcgg tgccatactt accgattgac ccctccgaca ttggccgtac atacgattcg
     3001 atcatccgca tcaactcgca atcgggaaaa ggcggtgtcg cgtatgtgat ggagcacgat
     3061 tttgggtgca aattacccaa aacgatgcac cctgaatttg ccgatgtggt gcaagccatc
     3121 gccgaaaaac agggcggcga agtgattccc agtgaaatca acgaggcgtt caagaacgaa
     3181 tacttagacc gtaaagaacc gttggagctc ttggggattt catccatcca cgagaatccg
     3241 gatacaggcg atgtgaaatt ggaactcaaa atgaaatcta attttaacca agaaggattc
     3301 ctttcgtcga gtgaaacgaa ttttgcgggt gaagggaatg gtcctttgga cgctgcacgc
     3361 aacgccatcc aacgcgcaac agacaataaa atttctttcc agatcaaaaa ctacagcgag
     3421 cattcgctgg gtgagggctc tgacgcgcgc gcgattgcgt acatccagat tgaatgtacc
     3481 gacaagaaga ctttttatgg agtggggata agccctaaca tttcacgcgc gtcgattgag
     3541 gcactcgtct cggcggtcaa tcgtgcgtgg caatctataa gaaattagtc tcagcgagtt
     3601 gacgcacagg ctccttggag ccttgcgttc aaaaaactcg ccgccctgac tcgaaaatag
     3661 agaatgcaca attatggtag cagaacaccc cgaatataaa cctggccttg atggcgttat
     3721 cgcgtgtatc actaaacttt cttggttgga taccgaaaaa gaacaaatcg ttgtacgcgg
     3781 ttacgacctc atcgagctca tccaaaagac atcgtacatt aaagttgcgt atcttcttat
     3841 ctataacaaa tttcccaccg acgcagaagc gaaagagttt gagcaaaaac tcatggcgca
     3901 ggcggatatt cccgaagaga tgtaccaaat ctttaaatta ctacctaaaa atactatggc
     3961 gatggatgca ctccgcaccg gcatttctat tttaggcggt tatgaagaac acgcagcgct
     4021 catggacact tcgacaaagt ctaacgaaga aaaagcgctc aaaagtttgg ctaaagcccc
     4081 cgcgattgcc gtcaacagct accgcgctat caccggccag ccgtttgtga aaccggataa
     4141 atcgttgggc tattcggaaa actttgttta catgattaaa ggttctaaac ccactgcagt
     4201 cgaaaccgac acattggata aaatcctcac cacctacatc gaacacgaaa tgcccaactc
     4261 gactttcgcg gtgcgggtgg tgagctcgac gctttcggat
//
LOCUS       DAOMPD010000211         4290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8543_length_4290_cov_2.337190, whole genome shotgun sequence.
ACCESSION   DAOMPD010000211 DAOMPD010000000
VERSION     DAOMPD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_4290_cov_2.337190"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..596)
                     /locus_tag="PLY93_08205"
     CDS             complement(30..596)
                     /locus_tag="PLY93_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69498.1"
                     /translation="MKKEEEKKYFPLKKRISHIFEFAFTMCTFCIVNLFPFSALHSVA
                     KTFRILFSPFLGSARKRIVAHVNVSLKIQDPKELTHFVNANLDNTIRNFLELMQAWKM
                     KKKKFIDKYVEMDDEVRAIAADKTQGVVFAEGHFGNWEIPIPTYASIGFKIFFSAQRL
                     SNPYVDAWTHRTRIGYGGGGPIYRKEAE"
     gene            complement(593..1816)
                     /locus_tag="PLY93_08210"
     CDS             complement(593..1816)
                     /locus_tag="PLY93_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP69499.1"
                     /translation="MRFFMEYQADILPVVEQGRIVGQIDRDQLIEAMSEFRPEGCSVT
                     IKDAESTDELLKYLDSSGQESMVTINRNFDFNALFSRAEIIQNSGRIPKIKVWAPPVE
                     PEPVDATFRNTQPIPSQEKIIEKKESFPTPQLTQKEEQIPTPLEHENAPRFSITRKAV
                     EINIKTKTETTKSTSKTVSIPHELPQISTEKNNDKSLATNLHRELERGRLAINTLATL
                     DLPLMACDGTGVELFHNRPFADIRLRDGLYLKTDTLIRRAKDAITESAHKGELDIDKA
                     LELKEAPRGFHIFCKAIRNYDDPSARAVGYIFWLTATTPNPTPIRSDAIFSLDPDTSF
                     SGRTLPEILAAEEARAMAWAMREANDNQSDAALLLGIPRQTFNYRYRKLMRQLSHLRK
                     NEKHTQNNPPIIDTP"
     gene            complement(1833..2711)
                     /locus_tag="PLY93_08215"
     CDS             complement(1833..2711)
                     /locus_tag="PLY93_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69500.1"
                     /translation="MPQNRVQILFNELEAKFAETEKSFLHAEALRHRIKGEYEAILEA
                     VRSAKKKFHMHPRATLVRRLSLMETLLSDLLLRSQGERRSFDALGFLLSRLRSNLASE
                     ILEPKLYQKIRNALEEEENFKRLRAKYPDSEAVITHKKNTLAIKEKESASKVIRLMLI
                     SHAGIHYAIPVKSILKKTNALSPVAAKMRDAQYTRMPLPVNGQKESGETIAVFFIDLK
                     GKKCYLHCDSYFTPVEIAQKILKKMLVFTQDKIAGKVEHRPFVSFYGRRFFVYGARLS
                     YTIRTGVRNHTHTSHE"
     gene            complement(2715..3341)
                     /locus_tag="PLY93_08220"
     CDS             complement(2715..3341)
                     /locus_tag="PLY93_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRP69501.1"
                     /translation="MATQKKSTPKKRTKRQPRPSTPHEVFPPLPTYSPSIVRKKFPIV
                     SWHEFIATGFFSGYLPKAPGTWGSLCAVFLFYFIAQFLPAQGSVSIGFLSLSWFALFL
                     GTLATFIGIYTSQKLADEWNEKDPGEIVIDEFAGIFFACLLIPATPLALLLAFLFIRV
                     FDILKPGPIASLQNLPGGRGIVLDDVLAGLFAAPLAFIFHWVFTKWIF"
     gene            complement(3341..3988)
                     /locus_tag="PLY93_08225"
     CDS             complement(3341..3988)
                     /locus_tag="PLY93_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRP69502.1"
                     /translation="MKERAKLPYDVDTTTNFQVYGGVKKGDSAARVAVVETHDEVLYY
                     GAQLAETFFHSSCGGTLASAEEVWGKNVPYLKIQSSSWCSTAPVYKWQVKIPYTEIGR
                     RLKLKGVKAMRIVERSKSKRVKTIQITTARGTKKIRGDKFRMALGAIKVRSTFFGVAQ
                     KGSAVILTGRGFGHGVGLCQWGAKLQADKHGTHYRRILEHYFPGTSLRTVNAQSV"
     gene            complement(3995..>4290)
                     /locus_tag="PLY93_08230"
     CDS             complement(3995..>4290)
                     /locus_tag="PLY93_08230"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRP69503.1"
                     /translation="TGVSANEFSFTAGSSTRRGTGAFTITEAGVFTPKGTFTFKRKSY
                     HGSLTVTREKGRWLYVNQVPMDEYLTSVVSHEMSPSWNKEALKAQAVVARTYL"
BASE COUNT         1029 a          935 c         1094 g         1232 t
ORIGIN      
        1 ggttctttgc ggcgcaagag cggaataaac tattctgctt ctttacgata gatggggcct
       61 ccgccgccat atccaatgcg agtgcggtgt gtccacgcgt cgacgtaagg attggaaagg
      121 cgctgtgctg aaaaaaatat tttgaagcca atcgacgcgt aagtagggat aggtatttcc
      181 cagttgccaa agtggccttc tgcaaacaca acgccttgtg ttttgtcggc ggcaatcgca
      241 cggacttcat cgtccatctc tacatactta tcgataaact tctttttctt catcttccac
      301 gcttgcatca actctaaaaa gttacggatt gtgttatcga ggttcgcatt cacaaaatgc
      361 gtgagttctt tcgggtcttg aattttgagc gatacgttca catgcgcaac gatgcgtttg
      421 cgtgccgagc ctaaaaacgg cgaaaagaga atgcggaatg tttttgctac cgaatgcaac
      481 gccgagaatg ggaataaatt cacgatgcaa aacgtacaca tcgtgaaggc aaattcaaaa
      541 atgtgtgaaa ttcgtttttt gagagggaag tattttttct cttcttcttt tttcatggag
      601 tatctataat agggggattg ttttgcgtgt gtttttcatt ttttcgtaga tgggataatt
      661 gtcgcatcaa tttacggtag cggtaattaa acgtttggcg cggtattcct aaaagtagcg
      721 ccgcgtccga ttggttgtcg ttagcttcgc gcatcgccca cgccattgcg cgcgcttcct
      781 cggcggctaa aatttcaggt agggtgcgcc ctgaaaaact tgtatcggga tcgagcgaga
      841 aaattgcatc ggagcggatg ggagtggggt tgggtgtcgt agcggtgagc caaaaaatat
      901 aacccaccgc gcgggcagaa gggtcgtcgt agttacggat tgctttacaa aaaatgtgaa
      961 agcctcgtgg cgcctctttt aattccaatg ctttgtcgat gtcaagttct cccttgtgcg
     1021 cactttcggt gatggcgtct ttggcgcggc ggatgagcgt gtcggtcttc aaatatagac
     1081 cgtcgcgcaa gcggatatcg gcaaatggac ggttgtggaa taattcaacc ccggtgccgt
     1141 cgcacgccat gaggggtaaa tccaacgtgg cgagggtgtt gatggctaaa cgaccgcgtt
     1201 cgagttcacg gtgcaaattt gtcgctagag atttatcgtt atttttttct gtacttattt
     1261 gagggagctc gtgggggata gacactgttt tagaagtact ctttgtcgtt tccgtttttg
     1321 tttttatatt gatttcgaca gctttgcggg taatcgaaaa acggggcgca ttttcgtgtt
     1381 caagaggagt agggatttgt tcttcttttt gtgtcagttg tggtgttggg aaagattctt
     1441 ttttctctat aatcttttct tgggacggta taggctgcgt atttcgaaaa gtagcgtcga
     1501 ctggttcggg ttccaccggt ggcgcccaca ctttaatttt gggaatacgc ccactgtttt
     1561 gaataatttc agcgcggcta aaaagcgcgt tgaaatcaaa attacggttg attgtcacca
     1621 tcgactcttg accgctgctg tcgaggtatt tcaagagttc gtccgtcgat tcggcgtctt
     1681 tgatggtgac gctgcaccct tcggggcgga attcgctcat cgcctcgatg agttggtcgc
     1741 gatcaatttg accgacgatg cgcccctgtt cgaccaccgg taaaatgtcg gcttggtatt
     1801 ccatgaaaaa gcgcatcaag cgatgtagct tctcattcgt gagatgtatg cgtgtggttt
     1861 ctgacaccgg tgcgaatagt ataactcaaa cgtgcgccgt acacaaaaaa gcggcgtccg
     1921 taaaatgaca caaaagggcg gtgctccact tttcccgcaa ttttgtcttg tgtaaatacc
     1981 agcatttttt ttagtatttt ttgcgcgatt tccacaggag taaaatagct gtcgcagtgg
     2041 agatagcatt tttttccttt aaggtctata aagaaaacag ctatcgtctc tccgctctct
     2101 ttttgcccgt tgacaggaag cggcatgcgg gtgtattgtg cgtcacgcat tttggcagcg
     2161 acgggactta aagcatttgt ctttttgaga atacttttta cagggatagc gtagtggatg
     2221 cctgcatgcg aaattaacat aagccggatt actttcgacg ccgattcttt ttctttgatt
     2281 gcaagagtat tttttttatg ggtgatgacc gcttcagaat cgggatattt tgccctcaaa
     2341 cgcttgaaat tttcttcttc ttcgagcgca ttacgaattt tttggtagag ctttggttct
     2401 aaaatttcgc tggctaagtt ggagcgtaaa cgactcagga gaaaccccag cgcgtcaaag
     2461 ctacgccttt cgccctgcga gcgtaacagt aagtccgaca acaacgtttc cataagcgag
     2521 agacgtctca caagagtagc gcgcgggtgc atatggaatt tttttttcgc gctacgtacc
     2581 gcctcaagaa ttgcttcgta ttcgcctttg atacgatgac gaagagcttc tgcgtggaga
     2641 aaactttttt cggtttcagc gaactttgct tcgagttcgt taaaaagaat ttgtacgcgg
     2701 ttttgtggca ttgtttagaa aatccatttt gtaaaaaccc agtggaagat aaatgcaagc
     2761 ggcgcggcaa aaagtcccgc gagtacgtca tccaaaacaa tgccacgccc ccctggcaaa
     2821 ttttggagtg aggcaatggg accgggtttt agaatatcaa aaactcgaat gaaaagaaac
     2881 gccaacagaa gcgctaatgg tgtggctggt atcaaaaggc atgcaaaaaa aataccggca
     2941 aactcatcga tgacaatttc tcctgggtct ttttcattcc actcgtcggc taatttttga
     3001 gaagtgtaaa tcccaatgaa agtggcgagg gttcccaaaa agagggcaaa ccacgacagc
     3061 gaaagaaaac cgatgcttac gctgccctgc gcgggcaaaa attgtgcgat aaaataaaaa
     3121 agaaaaacag cgcagagcga gccccacgtt cccggcgctt tgggtaggta gcccgaaaaa
     3181 aaaccggtgg ctataaattc atgccaactc acaatgggaa attttttacg gactatcgac
     3241 ggtgagtatg tcggcaatgg cgggaatact tcgtgaggag tcgatggcct cggttgcctt
     3301 tttgtacgtt ttttaggtgt ggattttttt tgtgtagcca ttagacgctc tgtgcattga
     3361 ctgttctcag cgaagtgccg ggaaaataat gttcaagaat gcggcggtag tgcgtgccgt
     3421 gtttatcggc ttggagtttg gcgccccact ggcaaaggcc tacgccgtgg ccaaacccgc
     3481 gaccggtgag aatgacggcg cttccttttt gcgccacacc gaaaaaggtc gagcgtactt
     3541 tgattgctcc caatgccata cgaaatttat ccccacggat tttttttgtc ccgcgcgctg
     3601 tggtgatttg tatcgtttta acgcgtttgg acttactgcg ttcgacaatc cgcatcgctt
     3661 taactccctt aagtttcaag cgccgtccga tttctgtata ggggattttc acttgccatt
     3721 tataaacggg ggcggtggaa caccacgacg aactttggat ttttaagtag ggtacattct
     3781 tcccccatac ttcttccgcc gaagcgagtg tgccgccgca ggaagagtgg aaaaatgttt
     3841 ctgcgagttg tgcgccatag tataaaactt catcgtgcgt ttcaacaacg gcaacgcgcg
     3901 cagcgctgtc gcctttttta acgccaccgt acacttggaa gttggtggtg gtgtcgacat
     3961 cgtaaggtaa tttagcgcgt tctttcattt tttgtcaaag ataagtgcga gcgacgacag
     4021 cctgtgcttt tagagcctct ttgttccacg aagggctcat ctcgtggctc accaccgagg
     4081 tcaagtattc atccatcggt acttggttta cgtaaagcca acggcctttt tcgcgtgtta
     4141 ccgtgagtga gccatggtaa cttttccgct taaaagtaaa agtgcctttc ggtgtaaaaa
     4201 cgcccgcttc ggtaatcgtg aaagcgcctg tgcctcggcg tgtactactg ccggcggtaa
     4261 aactaaactc attggcgctg acaccagtgg
//
LOCUS       DAOMPD010000212         4284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8565_length_4284_cov_2.612438, whole genome shotgun sequence.
ACCESSION   DAOMPD010000212 DAOMPD010000000
VERSION     DAOMPD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_4284_cov_2.612438"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLY93_08235"
     CDS             <1..587
                     /locus_tag="PLY93_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69504.1"
                     /translation="LAQKINYVSLGGGFGIKYEKIEGNGVDFNFANLASAVADIFKDD
                     QLEVHFEPGRFIAAPTGILLTRVRHLKERTGKVIAIIDAAMNDLIRPMLYEAYHPILS
                     VHRRGGEQLYDVVGPVCESTDTFTTKRTLATLVENDWLAIGYAGAYGASMSSNFNSRV
                     LLPELLLSANGELKFLRTPQTYDEMLANELVAQN"
     gene            703..2532
                     /locus_tag="PLY93_08240"
     CDS             703..2532
                     /locus_tag="PLY93_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRP69505.1"
                     /translation="MPSRLHDLSWGDVFYAFFMGLRFDTVVSGYLLALPFLLLSIGIF
                     ITRIERLFLKTAFIFIFLGGFIALWGGAADIPFFNQFFTRLNITAIEWMDTPLFVVSM
                     IFQEWRYAIYVGLFFILMVVFFIVGRSTYRRAAHLPHIEGNYALKTAATFVCVFLIFL
                     GIRGRVDEKSPIRVGTAYFSNNAFLNQLGLNPTFTFIRSALDAKEEKNKPIHLMDNQK
                     ALQLVQRYLHIDTPHKEIPLMRSIHTTGKENRINVVVVIMEGMSAFRLQHYNRTYNHT
                     PFLDSMAKRGLFFENTFTSGIHTFNGVFSTLFSFPAIYRQHPMKESAIFRYNGIGDTL
                     KLAGYSTSYFTTHDGQFDNIEGFLRANGFEHVITKANYPSDKIKTTLGVPDDYMFEFA
                     IPLLRKRAAEKKPFFAAFLTASNHGPYYLPDYFTPRAKKIDDQMIEYSDWSLKKFFTL
                     AQKESWYKNTLFVFIADHGASIGPTFDMPLGYNQTPLIFYKEGMNPQTITKIAGQIDI
                     YPTIMGFLNIGYINNTMGIDLRKENRDFILFNGDDKYGVANTRWLYYGHKDGRANLYD
                     YKRDGQDVLKKNPSTALVMKTYAEAHLQAFQYLLQNPQMLYSR"
     gene            complement(3101..>4284)
                     /locus_tag="PLY93_08245"
     CDS             complement(3101..>4284)
                     /locus_tag="PLY93_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRP69506.1"
                     /translation="TASKEGGASLNILAGLTAGNFSAYWMGIVIVVLMGISYFVAITG
                     LQQLMSPFAVAVFAFGLVAYGFLSMGPVTIAVDSYGPVTDNAQSVYELSLIEKVPNIQ
                     AEVKKDFGFDLNFDRAKYFLEANDSAGNTFKATSKPVLIGTAVVGATAMIFSIIMMLT
                     NNFSNQSAIGKLSILYAPFLLGLITGGAIIYWFSGAATQAVSTGAYRAVEFIKANIKL
                     ESTEKASIKDSKRVVEICTQYAQKGMFNVFLGIFFGTLAFAFLNEYFFIGYLISIAVF
                     GLYQAIFMANAGGAWDNAKKLVEVELQQKGSPLHDATVVGDTVGDPFKDTSSVALNPV
                     IKFTTLFGLLAVELAVVLKNPDGSTMMLRSVLSVAFFLLSAVFVWRSFYKMRIKESKT
                     L"
BASE COUNT         1148 a         1044 c          956 g         1136 t
ORIGIN      
        1 aactcgcgca aaaaattaat tatgtttcgt tgggcggcgg ctttggtatc aagtacgaaa
       61 aaatcgaggg gaatggtgtt gattttaatt ttgcgaattt ggcatctgcg gtggctgata
      121 tttttaaaga cgaccaactc gaagtacact ttgagcccgg ccgctttatc gctgcaccga
      181 cgggcatttt actgacgcgc gtgcgacacc taaaagaacg cacaggcaaa gtgattgcca
      241 tcatcgacgc cgcgatgaac gatttaatac gccccatgct ttacgaagct taccatccca
      301 ttttgtcggt gcaccgtagg gggggtgagc aattatatga cgttgttgga ccagtgtgcg
      361 aatcgaccga taccttcacc acgaagcgta cgctcgccac tcttgtcgaa aacgattggc
      421 tcgcgattgg ttatgcgggc gcgtacggtg cgtcgatgag ctcgaatttt aactcgcgcg
      481 ttttattgcc agagcttttg ctttccgcaa acggcgaact caaattttta cgtacaccgc
      541 aaacgtacga tgagatgctg gcaaatgaat tagtagcgca aaattaacgc gatgcaaatc
      601 cggcacacga ttttccaact ccgctcggtg tacgtcttga gccgcgtgta tctggtgttc
      661 gtgttttttt tcaccacatt tcgggtgctc ttgtttttga gcgtgccctc acggcttcac
      721 gacctttctt ggggcgatgt gttttacgca ttttttatgg ggctacgttt cgatacggta
      781 gtttcgggtt acttgctcgc gctgccgttt ttactgctca gcatcggtat ttttattaca
      841 cgtattgagc gcctattttt gaaaacggcg tttattttta tttttctcgg cgggtttatc
      901 gcgttgtggg ggggcgcggc ggacattcca tttttcaatc aattttttac gcggctcaac
      961 atcaccgcaa tcgagtggat ggacacccct ctttttgtgg tgtcgatgat tttccaagaa
     1021 tggcggtatg caatctacgt cggtttgttt tttattttga tggttgtttt ctttatcgta
     1081 gggcggagta cataccgccg cgccgctcat ttgccgcaca tcgagggcaa ctatgcgctc
     1141 aaaacagcag caaccttcgt ttgtgtgttt cttattttcc tcggcattcg aggccgcgtc
     1201 gatgagaaaa gccccatccg tgtgggcacg gcgtatttta gcaacaacgc atttctaaac
     1261 cagttgggcc tcaatcccac gtttactttt attcgcagcg cgcttgatgc caaagaagaa
     1321 aaaaataaac ccatccattt aatggacaac caaaaagcgc tacagctcgt gcagcgttat
     1381 ctccacatcg atacgcccca taaagaaatc ccactgatgc gcagcatcca caccaccggc
     1441 aaagaaaacc ggataaacgt ggtggtggtc attatggaag gcatgagtgc gtttcgtttg
     1501 caacattaca accgcacata caaccacaca ccgtttttag acagcatggc caaacgtggg
     1561 ttattttttg aaaatacttt tacatcggga atccatacgt tcaacggagt cttcagtacg
     1621 ctcttttcat tccctgcaat ttaccgccag catccaatga aagaaagcgc gatttttcgt
     1681 tacaacggca tcggcgatac gttgaagctt gcagggtata gcacgtcgta ctttaccacg
     1741 cacgacggcc agttcgacaa catcgaaggc tttttacgcg cgaacggttt tgaacacgtc
     1801 atcaccaaag caaactatcc ttcggataag attaaaacca ctctcggtgt gcccgacgat
     1861 tacatgtttg agtttgcgat accccttctg cgcaaacgcg ccgcagagaa aaaaccattc
     1921 ttcgcggctt tcctgaccgc ctccaaccac gggccgtatt acctccctga ttatttcacg
     1981 ccgcgtgcga aaaagatcga cgatcagatg attgaatact ccgattggtc gctgaaaaaa
     2041 ttttttaccc tcgcgcaaaa agagtcgtgg tataaaaata cactgtttgt ttttatcgcc
     2101 gaccacggcg cgtccattgg ccctacattc gacatgccat taggctataa ccaaactccg
     2161 cttattttct ataaagaagg gatgaacccc caaaccatca caaagattgc cggccaaatc
     2221 gatatctatc ccaccattat gggattccta aatatcggct acatcaataa caccatgggg
     2281 attgacttac gcaaagaaaa tcgcgacttt attctcttta acggcgacga caagtatggc
     2341 gttgcgaaca cccgttggct ttattatggc cacaaagatg ggcgcgcaaa tttatacgac
     2401 tacaaacgtg acggacaaga cgtcttgaaa aaaaatccat cgacagcact cgtcatgaaa
     2461 acgtacgcag aggctcactt gcaagcgttc cagtacttgt tgcagaatcc ccaaatgcta
     2521 tactcacggt agcaagtaac gggcgattgc gattgacttt ttaagatatc acgttcctgt
     2581 gcaaccgcga caatatcttt tttacattga tatcatgcct ttttctgagc acaatgaaat
     2641 tccttaaatc atagttatgt cgggtcttat attagggttt agatttcgag gccttggcca
     2701 caaaattatg tcacattagg gggtaactat accgattttt taagattatg tcacattagg
     2761 gagtaactat accgattttt tgagattatg tcacattaag gggtaactat gttccacgag
     2821 tgctggagtg gccaaaatta gggtcaattt tgaggaaaaa ctttctccta tacatactat
     2881 cgatttttcg agccatttag ccatcgctga gcagcacgtg cttggtagat tacaaatgag
     2941 ttttttctgt catacggtca cttcgagacc tctggcagca gagacttact tgggcgttgc
     3001 agtgaccgtc aaaactgaaa cacaaccgca actagtgcgg tatgaatcat ccgaaaacgg
     3061 agactcacac cgcttgttac aacaagactt tttagcggac ctaaagtgtt ttactttctt
     3121 tgatgcgcat cttgtagaaa gaacgccata caaaaacagc cgagagtaaa aagaacgcaa
     3181 cggacaaaac actgcgcagc atcatggtcg atccgtctgg atttttgagc acaacagcga
     3241 gctccaccgc caagaggcca aaaagcgtcg tgaacttaat cacgggattg agcgccaccg
     3301 acgatgtgtc tttgaacgga tcacccacag tgtcccccac aacggttgca tcatgcagcg
     3361 ggctaccctt ttgttgcagt tcaacttcga cgagcttttt cgcgttatcc catgcaccac
     3421 cggcgttcgc catgaagata gcttgataaa gaccaaacac cgcaatggaa attaaatacc
     3481 caataaagaa gtactcgttg aggaacgcaa acgccaacgt gccaaagaaa attcccaaaa
     3541 agacgttaaa catccctttt tgagcgtatt gagtacaaat ttcaacaacg cgtttggagt
     3601 ccttaatcga cgctttctct gtactttcga gcttgatgtt cgctttaata aattccactg
     3661 cacggtacgc gccagtcgaa accgcttgcg ttgccgcgcc cgaaaaccaa tagataattg
     3721 cgccgccggt gatgagaccg agcaagaacg gcgcgtaaag aatagagagt ttaccaatcg
     3781 ccgattggtt actgaagttg ttggtgagca tcatgatgat agagaaaatc atcgctgttg
     3841 cgcccacaac cgccgtaccg atgagcactg gtttcgacgt tgctttaaac gtgttacctg
     3901 cgctatcgtt cgcttcgaga aaatattttg ctctatcgaa gttgagatca aaaccaaaat
     3961 ctttttttac ttctgcctga atgttgggta ctttctcaat gagcgagagt tcgtacaccg
     4021 attgcgcgtt gtcggtaaca gggccatacg aatcgacagc aatcgtgact ggccccatac
     4081 tcaagaagcc atacgccacc aagccgaatg caaaaaccgc cacggcaaaa ggactcatga
     4141 gttgctgcaa gccagtaatg gcgacaaagt aagagatacc cataaggacg acgatgacta
     4201 tccccatcca gtaagcggaa aaattaccgg ccgtgagacc cgccaaaatg ttgagcgacg
     4261 cgccgccttc tttagaggca gtca
//
LOCUS       DAOMPD010000213         4281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8573_length_4281_cov_2.259584, whole genome shotgun sequence.
ACCESSION   DAOMPD010000213 DAOMPD010000000
VERSION     DAOMPD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_4281_cov_2.259584"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..650
                     /gene="yajC"
                     /locus_tag="PLY93_08250"
     CDS             219..650
                     /gene="yajC"
                     /locus_tag="PLY93_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRP69507.1"
                     /translation="MNEAMFTIAQAASVPVTGAPAVSAAAPAKAATTATTSAPASTAN
                     QPQPDMSSTLSSFALPVLMIVVFYFLLIRPQQKREKEKRKQLNAMQKGDRVLTAGGIY
                     GTIVGVKQEENLAIVKIADDVKVEVAISTLSGVVTKDQNSK"
     gene            663..1241
                     /locus_tag="PLY93_08255"
     CDS             663..1241
                     /locus_tag="PLY93_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69508.1"
                     /translation="MRFLLYITLVFVCLPRAEYAGDTFNVESAFFLAQNLFDDDVPPL
                     APTDKPKEKAVTAASEKTTHKTVKKKKRKKKVKKKATSTAENEAVKTPSYSTAALARS
                     SYEWAPESEALSLTTEAKGVPLFNAAPVNKPTAQSQSSSVEKSNETLAEKKENFKISR
                     IPVTQVLIVAAFVLLFLLYRYRVSKQMKHKKY"
     gene            1251..1805
                     /locus_tag="PLY93_08260"
     CDS             1251..1805
                     /locus_tag="PLY93_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69509.1"
                     /translation="MSAQVRFFMILIVIALSVALIWPNIGERTIRLYPNETLSETKRD
                     EVLNNVFKYVNEHYKGKYTPEIRTGKPIDNPKGVQEKYLSITGKFVQAAFLNELGRIE
                     GVDASRMALEPMWVERNLKAKPFKLGLDLQGGMNLVLEGDFEKVATRIKEVYPPEMIA
                     ELKRKKKQKKIQKRKSRLRQSWHR"
     gene            1814..>3051
                     /gene="secD"
                     /locus_tag="PLY93_08265"
     CDS             1814..>3051
                     /gene="secD"
                     /locus_tag="PLY93_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRP69510.1"
                     /translation="MDLSPEKRKSDMEGVLQILSSRINQTGTSEPLIRLQGNEQIEIS
                     LPGVANPDQAKQLIASTARVTYHLHDPNGEEQRYTQLANAEFSEYMKLETDVQRLKFL
                     KTIETKINLPKKFALMVMYGKNPNMTKSGQHLEPESFLVVDKEPVMTGDAMSRNVYED
                     RNPEDGRIVVSFELTSEGADKFGKITTENQGKLLAIAIDDKIRSAPRIQGPILGGRGQ
                     ITGDFDVQEARSLAIVIREGALPVPLNIVEERSVGPTLGQAAIDSGLKAISIGFILVF
                     AYMLLYYHGSGFIASVGLLINLLLMAAIFALMDFTITLPGLAGVVLTMGMAVDSNVII
                     YKRIREELARGKSVKLAVAAGFERATLTIIDANLTTLIAAVILASKLGVGPIKGFGVT
                     LFIGIVTTLFTALIVTRAVM"
     assembly_gap    3052..3061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3146..4108
                     /gene="secF"
                     /locus_tag="PLY93_08270"
     CDS             3146..4108
                     /gene="secF"
                     /locus_tag="PLY93_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRP69511.1"
                     /translation="MNLNFHVMRFRWFNLTGSLLLMIGLLVFTFQKYGGFALSIDFAG
                     GIKLELTRVENLTLDSLRTLFEKEKINALVQLVDKTSGDRIKIEIGGNVQQDLTRRAA
                     AAKDELTKANYPVNAIDYTKYLIQTKLLPPNAAKIEFSAAEQVGPTVGKYMQTNAIYL
                     VMVALILISVYVALRFRFNFALGALLSSLHDLVITMGVIGVLQIPLSVPVIAALLTIL
                     GYSINDTIVIFDRIRENIHGKEDIGIEHVVDKSIMQSLTRTINTTTTTFIAIFPIYLL
                     GDDSLKDLAEVLLYGILIGTYSSNFVAAPIVVIWDNLMKRRRAS"
     gene            4105..>4281
                     /locus_tag="PLY93_08275"
     CDS             4105..>4281
                     /locus_tag="PLY93_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HRP69512.1"
                     /translation="MKLAIAKETASGEKRVALIPESVKKLKDKGFTLVVEKGAGEASF
                     ISDKEYVDAGATLVS"
BASE COUNT         1240 a         1030 c          946 g         1055 t
ORIGIN      
        1 ccgccagacg ctcaaaacca ttctcggttt cctcaaggcc agtatgcatc gaccccatga
       61 gcgatcggtt acgtagtgtg gtaaacccta aacttaaagg agataaaaaa tgaggatatg
      121 cacccatggg gcgttcttta aagtggaaac accggttcaa tggttttaca ctttatgtgt
      181 tgccttgacg cactgtgtca aaaatactcc ttgccgacat gaatgaagct atgttcacca
      241 ttgcgcaggc tgcaagcgtt cccgtgaccg gtgcgcccgc tgtaagcgct gccgctcctg
      301 caaaagcagc gacaacagcg accaccagcg cgcctgccag tacagccaac caaccccaac
      361 ccgatatgtc gagcacgtta tcgagctttg cactccctgt tttgatgatt gtcgtttttt
      421 atttcttgct tatccgcccg caacaaaaac gcgaaaaaga aaaacgtaaa caactcaatg
      481 caatgcaaaa aggcgaccgc gtattgaccg ccggcggtat ttatgggacc atcgttggag
      541 ttaaacaaga agaaaattta gcgattgtaa aaattgccga cgacgttaaa gtcgaagtcg
      601 ctatttcaac attgagcggt gtcgttacta aagaccaaaa ctcgaaataa agcagtcctc
      661 cgttgcgctt tcttctgtac ataactcttg tttttgtgtg tctgccgcgt gccgaatacg
      721 caggcgacac attcaatgtt gaaagcgctt tctttttggc acaaaatctt tttgacgatg
      781 atgtaccccc actggccccg acagataaac caaaagagaa agctgtaacc gctgcatcag
      841 aaaaaacaac gcacaaaact gtaaaaaaga aaaaaagaaa gaaaaaagta aaaaagaaag
      901 ctacgtctac cgctgaaaat gaagcagtca aaacaccgtc ttattcaacg gcagcattag
      961 cgcgctcgtc gtacgagtgg gcgcccgaaa gtgaagcgct ttcattaact accgaagcta
     1021 aaggcgtccc ccttttcaat gccgcaccgg taaacaagcc cacagcgcaa agtcaatctt
     1081 cctccgttga aaaatccaac gagacgcttg ccgaaaaaaa agaaaatttc aaaatttcac
     1141 gcatccccgt tacgcaggtt ttaattgtcg ccgcgttcgt gttattgttt ttgctctatc
     1201 gttatcgtgt ttcaaaacaa atgaaacata aaaaatacta aggaaatccc gtgagtgcac
     1261 aagttcgttt tttcatgatt ctcattgtca tcgctttgtc ggtggcacta atttggccaa
     1321 atatcggcga acgtacgata cgcctatacc ccaacgaaac gctttctgaa acaaagcgcg
     1381 acgaagtttt gaataatgtg ttcaaatacg taaacgaaca ctataaaggt aaatatactc
     1441 cagaaattcg tacaggcaaa cccatcgaca accctaaagg cgtccaagag aaatacctaa
     1501 gcatcaccgg taaatttgtg caagcggcct ttttaaacga acttggccgt atcgagggtg
     1561 tcgatgccag tcgaatggct cttgaaccca tgtgggtaga gcggaattta aaagccaagc
     1621 ctttcaaatt gggactcgat ctccagggag gaatgaactt ggtactggaa ggcgattttg
     1681 aaaaagtcgc tacacgcatc aaggaagtct atcctcctga aatgattgca gaactgaaaa
     1741 gaaaaaagaa acagaaaaaa atccagaaga gaaaaagtcg attgaggcaa agctggcatc
     1801 gataaaacaa gcaatggatt tgtccccaga aaagcgtaag agcgatatgg aaggggtatt
     1861 gcaaattctt agctcgcgta tcaatcaaac cggtacctct gagcctttaa tccgcctcca
     1921 aggtaacgag caaattgaga tctcgctccc cggcgtggca aaccctgacc aagccaaaca
     1981 gctcatcgct tcgacggcgc gcgtcactta tcacctgcat gatcccaatg gcgaagaaca
     2041 acgatacact caactggcca atgccgaatt ttctgaatac atgaaacttg aaaccgatgt
     2101 tcaaagatta aaatttttga aaacgatcga aactaaaatt aaccttccga aaaaatttgc
     2161 acttatggtg atgtacggaa aaaatccgaa tatgacaaaa tctggtcaac acttggagcc
     2221 agaaagcttt ttggtggtag ataaagaacc cgtaatgacg ggagacgcca tgagccgtaa
     2281 cgtgtacgaa gaccgcaatc ccgaagacgg gcgcattgtt gtttcgtttg aactcaccag
     2341 cgaaggtgcg gataaatttg gcaaaatcac aaccgagaac caaggtaaac tgctcgccat
     2401 tgcgattgac gataaaatcc gttccgcccc ccgtatccaa ggtcctattt taggcggccg
     2461 tggccagatt accggtgact tcgacgtaca agaagcgcgc tcgttggcga ttgttattcg
     2521 cgaaggggca ttacccgtgc cgctcaacat tgtcgaagag cgctcggtgg ggccaacttt
     2581 gggacaagcc gccatcgact ctggccttaa agccatttcg atcggtttta ttttggtctt
     2641 cgcgtatatg ctgctttact accatggttc gggctttatt gcgagcgtgg gtttattgat
     2701 taaccttctt ttgatggcgg caatttttgc actcatggat tttaccatca ccctccccgg
     2761 tcttgcaggg gtggtgctca caatgggtat ggcggtcgac tccaacgtga ttatctacaa
     2821 acgcattcga gaagaactgg cacggggtaa gtcggtcaaa ttggcggtcg cggcgggctt
     2881 tgagcgcgcc actctcacca tcatcgacgc caacctgaca acattaattg cggcggtgat
     2941 tcttgcgtcg aagttagggg tcggtcctat caaaggcttt ggcgttaccc tctttatcgg
     3001 tatcgtcacc acgctcttta ccgcgctcat cgtcacccgc gccgttatgg annnnnnnnn
     3061 ngcttgcgta ttcagttgaa attaaatcgc tttcgattgg gttcggtcag cacagaaatg
     3121 ccgcaaaact tacaggaggt aaagtatgaa cttaaacttt cacgtcatgc ggttccgctg
     3181 gttcaactta accggctctt tacttttgat gattggcctc ttagttttca cattccaaaa
     3241 atatggcggc tttgccctca gcattgactt tgcgggtggt atcaaactcg agctcacccg
     3301 cgttgagaac ctcacgttag attcgttacg cacacttttt gaaaaagaaa aaattaacgc
     3361 tctggtacaa ctcgtcgaca aaacgtcggg cgatcgtatc aagattgaaa ttggcgggaa
     3421 cgtccaacaa gacttaactc gccgcgctgc cgctgcaaaa gacgaactca ccaaagccaa
     3481 ctaccctgtg aatgcgattg actatacaaa atatctcatt caaacaaagc tactccctcc
     3541 caacgccgct aaaattgaat tttctgcagc agagcaagtg gggcctaccg taggtaagta
     3601 tatgcaaacc aacgcgattt acttagtcat ggtcgcgctc atccttattt cagtgtacgt
     3661 cgcattacgt tttcgattta attttgcgtt gggtgcgctt ctctcctcgt tacacgacct
     3721 tgtcatcacg atgggagtta tcggcgtgct acaaattccg ttgtcggtgc ctgtcatcgc
     3781 cgcacttttg accatcctcg gttattcgat caacgacacc attgtgattt tcgaccgtat
     3841 ccgtgaaaat atacacggga aagaagatat aggcattgag cacgtcgtcg ataaatcgat
     3901 tatgcaatcg ctcacacgaa ctatcaacac aactacgacg actttcatcg ccatttttcc
     3961 aatatatctt ttgggcgacg atagcctcaa agacttggca gaggttctcc tttacggaat
     4021 cttgattggt acttattctt caaactttgt agcagccccc attgtagtaa tatgggataa
     4081 tcttatgaaa cggagaagag cctcatgaaa ctggcaatag caaaagaaac agcatcgggg
     4141 gaaaaacgcg ttgcgcttat ccccgagagc gtaaagaaat taaaggacaa agggtttacc
     4201 cttgttgtag aaaaaggcgc aggcgaagcc tctttcattt cagataaaga atacgtagac
     4261 gccggagcca cactcgtgtc g
//
LOCUS       DAOMPD010000214         4252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8650_length_4252_cov_2.727901, whole genome shotgun sequence.
ACCESSION   DAOMPD010000214 DAOMPD010000000
VERSION     DAOMPD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8650_length_4252_cov_2.727901"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..396)
                     /locus_tag="PLY93_08280"
     CDS             complement(43..396)
                     /locus_tag="PLY93_08280"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRP69513.1"
                     /translation="MFIEILMQLPPVHEQLIVALDVPKITDAMHLVDTLGDTVSFYKV
                     GMQLYFAEGNAFIRTLQERQKKIFLDLKINDIPETVAKAVESIASLKVDYLTLFTGAA
                     QIKAATATLLSLHDF"
     gene            complement(386..1111)
                     /locus_tag="PLY93_08285"
     CDS             complement(386..1111)
                     /locus_tag="PLY93_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69514.1"
                     /translation="MKFFTRGAQICFFLYVVSFVLNAQPAPPAPEETMEDGYLALEGG
                     LGYNTYPTNNYFRLPITFTGGSRQREISTRFVLLSDALHASEYFSGRNEFLKPNSIYL
                     YNFGLLDIDYLSWYGRFWSLGLGIGAAHQGFLIAGVDKTAHAATLRLRAHGFIYWSSY
                     FATHANITLPVALYQSGTDRFNMLHGEINMLFDFKGRVRMPEPQSFMFSVSLQWDNVR
                     IRYAMHEYAQVELTPLFKVIIVY"
     gene            complement(1123..1506)
                     /locus_tag="PLY93_08290"
     CDS             complement(1123..1506)
                     /locus_tag="PLY93_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69515.1"
                     /translation="MSELRVPDIALPRNYVRVVHEEKITKEDVEKVRRALQERIDTLE
                     NELKREKEIAKKLSKSSAVLQDILTAPAFKALSLETRKSATETVKEKNASIAHVDEAP
                     VEYVDVTEIEISDTMVSQEESQSKK"
     gene            complement(1503..1805)
                     /locus_tag="PLY93_08295"
     CDS             complement(1503..1805)
                     /locus_tag="PLY93_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69516.1"
                     /translation="MRGEFTLSYHFFRQCDLRNHLELFLDIDGVSPLETWRHFTTRKS
                     PARPLRFTAAPEHRRVYLMFAGEVSGARGRLRILRRGKFIDNRSAQKKRETMKVVL"
     gene            complement(1807..2859)
                     /locus_tag="PLY93_08300"
     CDS             complement(1807..2859)
                     /locus_tag="PLY93_08300"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRP69517.1"
                     /translation="MIYGGQSGEHEISCISATYLEQTIRAAGFAPIPIYVDRHGVWHW
                     QSAVSRKPHENSINPAALVRLNDGVVLRSATNAVLVDFAFPIIHGPAGEDGSLQGYFE
                     VLGLPYAGTGVASSAVSMDKALMRAVFAAADLPQVKYFVIENAAKISIDTLDQRITST
                     MQYPVFIKPCNMGSSVGVHKVKSRAELTAALVDAARFDDHLLCEEGLSVRELEIAIAG
                     NYPEYITSGVGEIQVNHEFYSYDAKYVDPNGAVLLLKAPIDARTHAQIETLAKCAFKS
                     VRGEGFARIDFFLDKNSGKVYLNEINTLPGFTPISMFPQLFAAAGKSGAEMTRLIIRW
                     GEERCKRLFELRLAAR"
     gene            complement(2899..3228)
                     /locus_tag="PLY93_08305"
     CDS             complement(2899..3228)
                     /locus_tag="PLY93_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP69518.1"
                     /translation="MDISFGDVGNHKVVRVMGDVDLYNVGDLKRSVFELIDEGETESL
                     IIDMASVNYIDSSGVGALVAAQKKMKTQGGKFGLMALTEDVLNILKLATLDQFFKIYE
                     GEAELPQ"
     gene            complement(3246..3665)
                     /locus_tag="PLY93_08310"
     CDS             complement(3246..3665)
                     /locus_tag="PLY93_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRP69519.1"
                     /translation="MSRIQFAAVNEGQDIPELRVGPIAQMDLVRYAGASGDFNPIHND
                     VEFAKAAGLPGTIAHGMYIMALMGRSVTNWVSPTQVKAFAVKFKGMSLPGETMVFTGK
                     IKKKKEENGEKLVTVALTAANDKGEVKVSGDLVVKAD"
     gene            complement(3681..4133)
                     /locus_tag="PLY93_08315"
     CDS             complement(3681..4133)
                     /locus_tag="PLY93_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRP69520.1"
                     /translation="MAELSKDIVGRKMDPFTFTIERGKVKEFLLAIGEKNAIYWDTAV
                     AKAEGYTDTPIPLTFQTAFTFWGYGEKFWKDMDDFGIDTKRLLHMKEEYTYLHPIYPG
                     DTVHCNVEVVDVKVGKMNMVTFKNTMNDGKGKTFIEAEMAIVIRPEGM"
BASE COUNT         1061 a         1061 c          967 g         1163 t
ORIGIN      
        1 ataataattt ctccatgata attatataat aattatcaaa tattaaaaat catgcaagct
       61 gagtaaagtt gctgtggctg cttttatttg cgctgcgcct gtaaataacg taagataatc
      121 cactttgagc gatgcaatcg attcgactgc tttcgcaacg gtttcgggaa tgtcgttgat
      181 tttcaaatcg agaaagattt ttttttgtcg ttcttgaagc gtacgaatga acgcatttcc
      241 ttccgcaaag taaagctgca taccaacttt gtaaaacgac actgtgtcgc ccaaggtatc
      301 gactaagtgc atcgcatcgg ttattttcgg cacatcgaga gcgacgataa gctgttcgtg
      361 aacgggaggt aattgcataa gtatctcaat aaacaataat aactttaaat aacggcgtca
      421 actctacttg cgcgtattcg tgcatggcat agcggatgcg tacgttatcc cattgtagcg
      481 aaacagaaaa catgaacgat tgcggctcgg gcatgcgcac gcgccccttg aaatcaaaaa
      541 gcatgttgat ttcgccatgc aacatgttga aacggtcggt gccgctttga taaagcgcaa
      601 cgggcaaggt gatgtttgca tgtgtggcaa aataactgct ccaataaata aatccatgcg
      661 ctcgcaagcg cagcgttgcg gcgtgcgcgg tcttgtccac accggcgatg aggaatccct
      721 gatgtgctgc gcctattccc aaccccagcg accaaaagcg gccataccac gaaaggtaat
      781 caatgtcgag caacccaaaa ttataaagat aaattgaatt gggtttcaaa aattcgttgc
      841 gtcccgaaaa atattccgat gcatgcaacg catcggaaag caatacaaac cgtgtggaaa
      901 tttcacgttg gcgcgaaccg ccagtgaatg taatcggcaa ccgaaaataa ttgttcgtgg
      961 ggtatgtgtt gtaacctaag ccgccttcta aagccaagta accatcttcc attgtctctt
     1021 cgggggcggg tggtgcgggt tgtgcgttta atacaaaaga aacaacatag agaaagaaac
     1081 aaatctgtgc cccgcgcgta aaaaatttca tcccgtataa tttcactttt tactttgact
     1141 ttcttcttga cttaccatag tatccgaaat ttcaatttca gtcacgtcga cgtactccac
     1201 cggcgcttcg tcgacgtgcg ctatcgacgc gtttttttct ttcactgttt ccgttgcact
     1261 cttacgtgtt tcaaggctca acgctttgaa agcgggtgcc gtcaaaatat cttgcagcac
     1321 tgcacttgat ttactcaatt ttttggcgat ttccttttcg cgcttgagtt cgttttccag
     1381 cgtgtcgatg cgctcttgta atgcccgcct tactttttcc acatcttctt tagtaatctt
     1441 ttcttcgtgc accacgcgca catagttacg cgggagtgca atatccggca cgcgcagctc
     1501 gctcatagca ccaccttcat ggtttctctt ttcttttgcg cgctgcggtt gtctataaat
     1561 ttgccacgac gcaaaatgcg tagccgaccc cgcgccccgc tgacttctcc tgcgaacatt
     1621 aaatagacgc ggcggtgttc gggggcggct gtaaagcgta aaggacgcgc tgggctcttg
     1681 cgcgtggtga aatgccgcca agtttctaaa ggggagactc catcaatatc taaaaataat
     1741 tctaagtggt tacgcaaatc gcattgtcga aaaaaatgat acgagagcgt gaactcgccc
     1801 cgcatgtcaa cgcgccgcca aacgcagctc aaacaaacgc ttgcaacgtt cctctcccca
     1861 acggataatg aggcgcgtca tttcagcgcc cgacttaccc gccgccgcaa aaagttgcgg
     1921 gaacatgcta atcggtgtaa agcctggcaa ggtgttgatt tcattcaagt acacttttcc
     1981 cgaattttta tccagaaaaa aatcgatgcg tgcaaaccct tcgccgcgca ccgatttaaa
     2041 agcgcatttc gccagtgttt caatttgcgc gtgtgtacgt gcgtcgatgg gcgctttgag
     2101 taaaagcact gctccgttgg gatctacata tttggcatcg taagaataaa attcgtggtt
     2161 cacttgaatt tcaccaactc ccgatgtgat gtattcgggg taattcccag cgatggcgat
     2221 ttccaattcg cgcaccgaaa ggccttcttc gcaaaggaga tgatcgtcaa accgtgcggc
     2281 gtcgacaagc gcagcggtga gctcggcgcg gcttttcact ttatgcacac ccaccgagct
     2341 acccatgttg caaggtttta taaaaacagg atactgcatt gtgctcgtga tgcgctggtc
     2401 gagcgtgtcg atagaaattt tagccgcgtt ttcaatgaca aagtatttta cttgcggcaa
     2461 gtcggcagcg gcaaaaaccg cgcgcatgag agctttgtcc attgacacag cggaggaagc
     2521 aacgccggtg ccagcatagg gcaaccccaa cacttcaaaa tacccttgca acgaaccgtc
     2581 ttcaccggct ggtccgtgta taatcgggaa agcaaaatcg acgagcaccg cgtttgtcgc
     2641 cgagcggagt accacgccgt cgttcaagcg cacgagagca gcgggattta tactgttctc
     2701 gtgtggtttg cgtgaaaccg cagattgcca gtgccacaca ccgtggcggt cgacgtagat
     2761 ggggatgggg gcaaagcccg ccgcgcgtat cgtttgttct aagtacgtcg ccgagataca
     2821 cgaaatttcg tgctcgcctg actggccgcc ataaatcagc gcgacggttt ggatgggttt
     2881 aatgtgtact ccgccccgct attggggaag ctcggcctcg ccttcataaa ttttgaagaa
     2941 ctggtcgaga gttgcgagtt tcaagatgtt gagcacatct tcggtgagcg ccataagtcc
     3001 aaacttaccg ccttgggttt tcatcttttt ttgagcggcc accaatgctc ccacgccgga
     3061 tgaatcgatg taattgacac tcgccatgtc gatgatgaga gactcggttt cgccttcatc
     3121 gataagttca aaaaccgagc gctttaagtc acccacgtta tagaggtcta cgtcacccat
     3181 aacgcgcact actttgtgat taccaacgtc tccaaatgat atatccatga ttattatgac
     3241 gagcgttaat ctgctttgac aaccaaatca ccactgactt taacttcgcc tttatcgttg
     3301 gcggctgtca gcgcgacggt cacaagcttt tcgccgtttt cctctttttt ctttttaatt
     3361 ttaccggtaa aaaccatggt ttcgcccggg agactcattc ctttgaattt cacggcaaac
     3421 gctttcactt gtgtcggcga aacccagttg gtcactgagc gtcccatgag cgccatgata
     3481 tacatgccgt gtgcaatcgt accgggcaaa cccgctgcct ttgcaaactc aacgtcgttg
     3541 tggatggggt tgaagtcgcc cgatgcgccc gcgtaacgca cgagatccat ctgcgcgatg
     3601 gggcctacgc gtaactcggg gatatcttgt ccttcattga ctgctgcgaa ttgaattcta
     3661 ctcattatta gcctctttaa ttacatgcct tctggccgaa tgacaatcgc catttccgct
     3721 tcaataaagg ttttaccttt accgtcgttc atggtatttt tgaaagtcac catgttcatt
     3781 ttgcccactt ttacatcgac cacttcgacg ttgcaatgta ctgtgtcgcc tgggtaaatg
     3841 ggatggaggt atgtgtattc ttctttcatg tgcaaaagac gtttggtatc gatgccaaaa
     3901 tcatccatat ctttccagaa tttttcccca tatccccaaa acgtaaaggc agtttggaac
     3961 gttaatggaa tgggagtgtc ggtgtaccct tctgcttttg ccacagcggt atcccaatag
     4021 attgcatttt tttcgccgat tgctagtaag aattctttca ctttaccacg ctcgatggta
     4081 aacgtgaatg gatccatttt tcggccaaca atatctttac ttaattcagc catgttgcta
     4141 cttttgtagc gttacggtgc cgtcaagtta tttgaaactt agaactcatg aaccaacgag
     4201 cctgtgcgcc aactcgcaca ccaagccctt cttcccccct aaaaatcaaa at
//
LOCUS       DAOMPD010000215         4246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8674_length_4246_cov_2.695061, whole genome shotgun sequence.
ACCESSION   DAOMPD010000215 DAOMPD010000000
VERSION     DAOMPD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8674_length_4246_cov_2.695061"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /gene="hisG"
                     /locus_tag="PLY93_08320"
     CDS             complement(<1..349)
                     /gene="hisG"
                     /locus_tag="PLY93_08320"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRP69521.1"
                     /translation="MATSESQLRIALPKGRMSDESLEFFSSRGLCSIAKTTDGRELIL
                     HDEKNNIDFYLNRSKDVGAYVEQGAADLGIMGLDVLLEHSFDVYIPAVLPFGGCRLSV
                     AYPNDRSDWQKKRE"
     gene            complement(351..1106)
                     /locus_tag="PLY93_08325"
     CDS             complement(351..1106)
                     /locus_tag="PLY93_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69522.1"
                     /translation="MKSKHNHLSQTKIPLGDLHTEENTFLHNAMQWITNNPAALRRII
                     FSIIGLAIVVTVAFILYAAAKDEHSRRLHEALETYEQIKILPKGDARQAQMKNFGSTL
                     KEICEQPFSTIESGAACLSGGNAFLEVNDYPQAAALFARAASSYNSEPMISIARFMQA
                     QALEANREFDKALTLYKNLEKNFTVAKKQEMVIFHQARMLYYMGKYDEAEEKFVKLSR
                     ENEGKEFAAPSRNYISLLNAERGKQNTPIPLQK"
     gene            1200..2873
                     /locus_tag="PLY93_08330"
     CDS             1200..2873
                     /locus_tag="PLY93_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69523.1"
                     /translation="MRLKDYFILPLVLLSPLWTLQPNERISEDVGVYIEGAPLAESLP
                     TLKAVVTTVAGEGAWDLMASSFETRSGINIFDLKKLHEIGVDTKSPWGIAFNKETEIG
                     EKAYKPEFVGILPTLSGSSVYNLLKEKITEVNLDNLKEIENGKILSWGTEADPGFLIQ
                     TDDAILLSNNLEFAKKTQKRASSPIAKTHFYKDMRAYFIKKNANKQPLAAFYLNPKLL
                     VSLFDAQKEFIAQLQKGINEGSTDEAIVSEDSPLIKEISDNLEASGGAFIVQNNRLTS
                     YFSYKYKSGYLNDTTKFYPRILQVKAQPLTSDTLSTTPIHYGLLKLNVVAIFDLLKTL
                     SPLFNNKYTESIDKINDKLHVDIQKQVIDSFRGNFNFQIMSIPAPDKLQDITQWGLSG
                     SFGIKSGTSAGWLDFFKAVDKEAKKFAVKRKNKAKIKFTYENHDEGSFVLITLPPDVT
                     NSKKTTTIAILIREDEIIISNSKTGVLKVLKGSETTLSERLLRLPYDTTQAVFFVDLQ
                     QVLKALQKAKEAAAFKPYLAMLEKLKYFSITSNIEGDFAGAEFSLHLRK"
     gene            2875..3585
                     /locus_tag="PLY93_08335"
     CDS             2875..3585
                     /locus_tag="PLY93_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP69524.1"
                     /translation="MLSIENLTKEYGRRKAVNGISFSLKKGEITALLGVNGAGKTTTL
                     RMLTGYITPTSGSVLFEGQNVLTARENLLSHIGYLPESPALYTDMSVEDFLLYMYRLR
                     KATKENEKAAVERALKRTDLLERRHDYIASLSAGFRKRVGLAQALVHEPQVILLDEPI
                     ADLDPRQIREIKALLKDLKNDHAVLVSSHILKEVHEFADRIFLMHHGKILAESTTDAI
                     PADAFEAWFMRHTENEAA"
     gene            3572..3892
                     /locus_tag="PLY93_08340"
     CDS             3572..3892
                     /locus_tag="PLY93_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69525.1"
                     /translation="MKPLKFWGFLFFISYCTTTKFQVPEEKFFTDGYSLDKGYTQYVE
                     IVDDKTLALPVTGRFRQNCQTAQQKGSERFKQEKNIIKVESILLGEYFEANGACRVRI
                     ALKG"
     gene            3892..>4246
                     /locus_tag="PLY93_08345"
     CDS             3892..>4246
                     /locus_tag="PLY93_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRP69526.1"
                     /translation="MMIQHNTAVEFLNGHTIGADAPPFFIAGPCVIESEQLLNTVAKE
                     IRRVSDKYNVLILFKSSFDKANRSSIDSFRGPGIHEGLALLKSTAAKYNLPTLTDIHT
                     PEEGVIAASYVDMLQI"
BASE COUNT         1226 a         1050 c          877 g         1093 t
ORIGIN      
        1 tctctcgctt tttctgccaa tccgaccgat cattgggata ggccaccgaa agcctgcatc
       61 caccgaaagg cagcacagcg gggatataca cgtcgaaact gtgttccaac agcacgtcga
      121 gtcccatgat gcctaaatcg gctgctcctt gctcgacgta tgcccccacg tctttgctac
      181 ggtttaaata gaagtcgatg ttattttttt cgtcgtggag aatgagttca cgtccgtcgg
      241 tggttttcgc aatgctgcac aagcctcggc tcgaaaagaa ctcaaggctt tcgtcgctca
      301 tgcgcccttt gggaagcgcg atacgtaatt gagactcgct tgtggccaaa ttatttctgc
      361 aacggtatgg gagtattctg ttttccccgc tcggcattta aaagagaaat ataattgcgg
      421 ctgggggcgg caaactcttt gccttcattt tcgcgcgaaa gcttcacaaa tttctcttcc
      481 gcctcgtcgt atttacccat gtagtaaagc atccgcgctt gatggaaaat taccatttct
      541 tgttttttag ccacagtaaa gtttttctct aaatttttgt aaagggttaa cgctttatcg
      601 aattcacggt ttgcctcgag cgcttgcgct tgcataaatc gcgcaattga aatcatcggc
      661 tcgctattgt aggaggacgc tgcgcgggcg aacaacgccg ccgcttgagg atagtcgttc
      721 acttctaaaa aggcattccc accgctcaaa caagcggcgc cgctttcaat cgttgaaaat
      781 ggttgttcgc aaatttcttt aagtgtacta ccaaaatttt tcatctgcgc ttgccgagca
      841 tcccccttcg ggaggatttt aatttgctcg tacgtttcaa gcgcttcgtg gagtctgcgg
      901 ctgtgctcat ctttggctgc tgcgtataaa ataaaggcca cagtaacgac aatcgcaaga
      961 ccaataatag aaaaaatgat ccgccgcagc gccgcagggt tatttgttat ccactgcatg
     1021 gcattatgca aaaatgtatt ttcttctgta tgcaggtctc ccaagggaat ttttgtttga
     1081 cttaaatgat tatgctttga tttcactaat gtgctccacg aaaaaacaca ataacgaaga
     1141 acgagatacc gtgtcgagaa atacgcttgt cggctggtta cgccgccaat tttggcataa
     1201 tgcgattaaa agattatttc attctaccgc tcgttttact ttcaccgctt tggacactcc
     1261 aacccaatga acgcatctca gaagatgtcg gtgtttatat cgaaggcgct cctttggctg
     1321 aaagtctacc cactctcaaa gcagtcgtca ccacagttgc cggtgaaggc gcctgggatc
     1381 tcatggcgtc gagttttgaa actcgttctg gaatcaatat ctttgatttg aaaaaactac
     1441 acgagatagg ggttgataca aaatcacctt ggggaatcgc attcaacaaa gaaactgaga
     1501 ttggagaaaa agcgtataaa cccgaatttg tcggtattct gccgacactt tcaggctcta
     1561 gcgtctataa tttacttaaa gaaaaaatta ccgaggtcaa tctggataac ttaaaagaga
     1621 ttgaaaacgg caaaatcctc tcttggggta ctgaagcaga tcccggattt ttaatccaaa
     1681 cggacgatgc aatactttta tctaacaacc ttgagtttgc caaaaaaact caaaagcggg
     1741 cttcttcccc catcgcgaaa actcattttt acaaagatat gcgcgcttac ttcataaaaa
     1801 agaacgcaaa caaacaaccg ttggcggctt tttatctcaa ccctaaactc cttgtctcac
     1861 tcttcgatgc acaaaaagaa tttatcgccc agttacaaaa aggtatcaac gagggcagca
     1921 ccgatgaggc gattgtttca gaagactcgc cgctcattaa agaaatcagc gacaatttag
     1981 aagcatcggg aggagctttt atcgtacaaa acaaccgact tacatcttat ttttcttaca
     2041 aatataaatc gggttatctg aacgatacga ctaaatttta tccccgcatt ttacaagtga
     2101 aagcacaacc tttaacttca gacaccctat cgacaacccc tatccactac ggcctcctca
     2161 agctaaacgt cgtcgcaatt tttgatttac tcaaaacgct gagccccttg ttcaataaca
     2221 aatatacaga atcgatcgac aaaatcaatg acaaattgca cgtcgatatc caaaagcaag
     2281 tgattgactc atttagggga aactttaact tccaaattat gtccatcccc gcacccgata
     2341 aactacaaga cattacccaa tgggggttga gcggtagctt tggcattaaa agcggtacca
     2401 gcgccggctg gcttgatttt tttaaagcgg tcgataaaga agccaaaaag tttgctgtta
     2461 aaagaaaaaa taaagcaaaa ataaaattca cttatgaaaa ccacgacgaa ggtagttttg
     2521 tcctcatcac gttgcccccc gatgtaacaa actctaagaa aacgaccacc attgcgattc
     2581 ttatccgcga agatgaaatt attatttcca acagcaagac aggtgtactc aaagttctca
     2641 agggcagcga aactacttta agcgaacgct tgctgcgctt accgtacgac accacccaag
     2701 ctgttttctt tgtcgactta caacaagtct tgaaggcgct tcaaaaggca aaagaagccg
     2761 ccgctttcaa accgtatctc gcaatgcttg aaaaattaaa atacttctct atcacttcca
     2821 atatcgaggg tgattttgcc ggcgctgaat tttcattaca cttaaggaaa taacttgctc
     2881 tcgatagaaa atctcacgaa agaatatggc cgccgtaaag cggtcaacgg catttcgttt
     2941 tcactcaaaa aaggggaaat caccgcgctc ttgggcgtca acggagcggg caaaaccacg
     3001 acgctgcgca tgctcaccgg ctacatcaca cccacctctg gcagtgtttt attcgagggg
     3061 caaaatgtgc tcacggcgcg tgaaaaccta ttaagccata tcggttatct acccgaatcg
     3121 cccgcgctct acaccgacat gagcgtcgaa gattttcttc tttatatgta ccgcttgcgt
     3181 aaagcgacga aagaaaatga aaaagccgct gttgagcgcg cgctcaaacg caccgattta
     3241 ctcgagcgcc gacacgacta catcgcatcg ctttcggcgg gctttcgcaa gcgcgttggc
     3301 cttgcgcagg cgcttgtgca cgaaccgcaa gtcattctgc tcgacgaacc aatcgccgac
     3361 ttagaccctc ggcaaattcg cgaaattaag gcgctgctca aagacttaaa gaacgaccat
     3421 gccgttttag tctcaagcca catcctcaag gaagtgcatg aattcgccga ccgtatcttc
     3481 ttgatgcacc acggtaaaat tcttgccgaa agcacaacag acgcaatccc tgccgatgca
     3541 tttgaggcat ggtttatgcg ccacaccgag aatgaagccg cttaaatttt ggggatttct
     3601 tttttttatc tcttattgca cgacgacaaa attccaagtg cccgaagaaa aattttttac
     3661 cgatggatat tccctcgata aaggctatac acaatacgtg gaaattgtcg acgataaaac
     3721 gctcgctttg cctgtcaccg gacgcttccg ccaaaattgt caaacggcgc agcaaaaggg
     3781 aagcgaacgc tttaagcaag aaaaaaacat aataaaagta gaaagtattc ttttaggcga
     3841 atactttgaa gcaaacggcg cttgccgcgt gcgcattgca ctgaaaggat aatgatgatt
     3901 caacacaaca ccgccgttga atttttgaac ggccacacca tcggcgcaga cgccccacca
     3961 ttttttatcg caggtccctg tgttatcgaa agcgaacaac ttttaaatac cgtggccaag
     4021 gaaatccgac gcgtgagcga taaatacaac gtgctcattc tttttaagtc gtcgttcgac
     4081 aaagccaacc gttcgtcgat cgactccttc cgtggcccgg ggatccacga aggacttgcg
     4141 cttctaaaaa gcaccgcagc aaaatataac ctccccactc tcaccgacat ccacacccct
     4201 gaagagggtg taattgcggc aagctatgtg gatatgctac aaatcc
//
LOCUS       DAOMPD010000216         4240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8688_length_4240_cov_2.629630, whole genome shotgun sequence.
ACCESSION   DAOMPD010000216 DAOMPD010000000
VERSION     DAOMPD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8688_length_4240_cov_2.629630"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..2240
                     /gene="ppk1"
                     /locus_tag="PLY93_08350"
     CDS             216..2240
                     /gene="ppk1"
                     /locus_tag="PLY93_08350"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRP69527.1"
                     /translation="MHPAERYLHRELSWLSFNERVLAEAQKVTNPILERVRFLAITQT
                     NLHEFFMVRVASHKKKIYQAVLEEYTEGKNTYKTLAAIKQQIDNFQKNAVHLWQTNLK
                     STLKKVGLPFVPFTSCYKSKITLTALRQYVELSILPSLTPVEINKKYRAAHLESGVFY
                     LWVRLQSTSTHAQTRTVCIRVPKNLPGVLSLPHSKTTRYVLLHEVIRYFLFLLFPQEI
                     IQATHFFRITRGSEPLVHEDLSTPLPSTFLSQHKTQGDAVCLEVDKKMPETAWAFLQN
                     ELSLEDDDIYPLPVVFPHDTFESFLKNINLPSHFFSPLSTRIFKEQYLFSALKKRDIL
                     LHHPFDSFSIVEKFVSEAVNDSATTAIYATFYRIGEASAIMDALLTAAQHNIEVTILI
                     ELRARFDEETNVRWAQRMKDTSIRVIHGFADYKTHCKTILIERKEKNQIVRYAHLSTG
                     NYNAATARLYTDFGLLPANPTLTADIAKLFSSLTTYSTLPAMEKLIVAPAHLKEQLLF
                     LLRREKKNARLGKKAIITAKMNALVDRSIIDALYDASAAGVKITLFVRGICCLRPQMP
                     GLSENIRVMSIVGRFLEHSRVLYVHNDGQDEVYLSSADWMPRNFERRIELMFPVESAP
                     LKQRIINEALTLSVRRDQSVYELDKNGDYRLQEFSCSHGASSSTNGGNPV"
     gene            complement(2198..3343)
                     /locus_tag="PLY93_08355"
     CDS             complement(2198..3343)
                     /locus_tag="PLY93_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69528.1"
                     /translation="MVVLHALFLLRREEIKSYFFIFMIALSFLSCADFERPERNAAPA
                     NGVVLIVSSDFSTGFLSALIPTTNTVVKDILPLFNDSTLRYSASEQATFVVQRLGSDS
                     LRRLNNTIEYTTAFERSNPQDIAFLGSGQAAVSFFNRNSIGIVSTQNGNSLGNIDLSS
                     YADADGYAEVGALFYTGGFLYATLGRLNRTATDATWPPVGDSYLLKIDVVSRSVLVET
                     LLTHANPVSRLHYNAARNSLAFAAPARFYFDAALDGACLEYSLTTDTLLTPPITEAQA
                     GLEISDCEIRSDGSGVFIGNNLSRDSVLVLFDSTTHTVTRVAASLSSTNGGYFSNFLL
                     HSNGKLYLADRNIYAPGIRVFSGVGLTEETTHAIYTGLPPFVLEEAP"
     gene            complement(3337..>4240)
                     /locus_tag="PLY93_08360"
     CDS             complement(3337..>4240)
                     /locus_tag="PLY93_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69529.1"
                     /translation="WQGAGSASFDSTFFRVALQTSGNVNHDRAEAGTFYGSLAKTFNE
                     TEVGASGYFALRPLAFWYPGNDPTYPLLEFYAQVSSTYRNPTLYERFGDNVFVTPSEK
                     LKSEKAITNAGGVRGSFMCFKNLSCSWRTEAFLTGAHYYIIFTQNSSRTLIAVNASRA
                     QILGIENEVLLNWPERFLLTLRYTYLDARDYGNIPYYQDKFLPMRPRHHAVGVITVPY
                     RAFRFMGSVEYRGAVFRDRYNSYSFYLASKTLVDAGVDLILDSYARHVFNFTVKNIFD
                     DQEVDMIGYTLPGRYFLVKWTAAW"
BASE COUNT         1220 a         1040 c          930 g         1050 t
ORIGIN      
        1 gggtagaaat ctgccatggc gggcaagact cttgttaagc ccgacaggct gctagggcaa
       61 gtgttttcaa taattatacc ttcattacga ttgaatggtc atctaccgcc tttgaaatcc
      121 gcgaacgcat tgttttcctg aattcacctc ccctcctatc cagctaacgt acaattcctt
      181 tacacctcac cttcacgcta aaaacagaag aggctgtgca tcctgctgaa cgctatctcc
      241 accgtgaact atcgtggctc agcttcaacg aacgggtgct tgccgaagcg caaaaagtaa
      301 ccaacccgat tttagaacgc gtgcgctttc tcgcaatcac acagacaaat ctccacgagt
      361 tttttatggt gcgcgtggca tcgcataaaa aaaagatcta ccaagctgtt cttgaagaat
      421 atacagaggg taagaatact tacaaaacgc tcgcagcaat aaaacagcag atcgataact
      481 ttcaaaaaaa tgccgtccac ctatggcaaa caaatctcaa aagtacactt aaaaaagtgg
      541 gattgccttt tgtaccattt acatcttgct ataaatcaaa aatcaccctc accgctttac
      601 gccaatatgt agaattatca atcttaccgt cgctcacacc ggtagaaatc aacaaaaaat
      661 acagagctgc gcatcttgaa agcggcgtgt tttatctttg ggttcgtttg caaagtactt
      721 caacacatgc ccaaaccaga accgtttgca ttcgggtacc taaaaattta cccggagtgt
      781 tatcgctccc ccattccaaa acaacacgct atgttttatt gcacgaggtc attcggtatt
      841 ttttatttct tcttttcccg caggagatta ttcaagctac acattttttt cgtatcacac
      901 gtgggagcga accgttagtt catgaagatt tatccacacc actgccttcc acttttctgt
      961 cgcagcataa aacacaaggg gatgcggtat gcctcgaggt cgataaaaaa atgccagaaa
     1021 cggcgtgggc atttttgcaa aacgagttat cgttagaaga cgatgatatc tatccattac
     1081 ctgtcgtatt tccgcacgat acgttcgaga gtttcttaaa aaatataaat ttaccctctc
     1141 attttttctc accactttct acacgaatat tcaaagagca atatttattt tctgccctaa
     1201 agaaaaggga tattttgtta catcaccctt ttgattcctt ttcaatcgtc gaaaaatttg
     1261 tgtcggaagc ggtcaacgac agtgcaacaa ccgctatcta cgcgacattt taccgcattg
     1321 gcgaagcatc tgcaatcatg gatgcactac ttaccgctgc acaacacaac atagaagtaa
     1381 caatacttat cgagctgcgc gcgcggtttg acgaagaaac gaatgtgcgt tgggcgcagc
     1441 ggatgaagga tacctcgatc cgcgtcatcc acgggtttgc agactacaag acccactgca
     1501 aaacgattct tatagaacgc aaagaaaaaa atcaaattgt gcgttatgcg cacctttcaa
     1561 cgggaaacta caacgcagcg accgcccgac tttataccga ctttggactc ctcccggcaa
     1621 acccgacttt aactgcggac atcgcaaagc ttttcagttc attgacgaca tactcgacac
     1681 ttcccgccat ggaaaagctc attgttgccc ccgctcatct caaagaacag ctcctctttc
     1741 ttttacgtcg tgaaaaaaag aacgcacgct taggtaaaaa agctatcatc actgccaaga
     1801 tgaatgctct ggtcgatcgc agcattattg acgcactata cgacgcgtcg gcagcggggg
     1861 taaaaatcac cctttttgtg cgcggcatct gttgtttaag gccacaaatg cctggcttgt
     1921 cggagaacat ccgcgtgatg agcatcgtgg gccgtttttt agaacacagc cgcgtgcttt
     1981 acgttcacaa cgacggtcaa gacgaagttt atctctcgag tgcagactgg atgccgcgca
     2041 attttgaacg acgcattgag ttgatgtttc ctgtggaaag tgctcctctc aaacagcgca
     2101 tcatcaacga ggcgttgact ctttctgtcc gccgcgacca gagtgtgtac gagttagata
     2161 aaaacggcga ttatcgcctt caagaatttt cctgctctca cggcgcctct tcaagtacaa
     2221 acgggggtaa tcccgtataa atggcgtgcg tagtttcctc ggttaatccc actccactaa
     2281 aaacacggat gccgggtgcg tagatgttgc ggtcggcgag ataaagctta ccattcgagt
     2341 gtaataaaaa atttgaaaaa taaccgccat tcgtcgaaga taaactggct gctacacgcg
     2401 tcaccgtatg cgtcgtgcta tcgaaaagca caaggacact atcacggctc aagttgttgc
     2461 cgatgaatac accgctccca tcgctacgga tttcgcaatc ggaaatttca agcccagctt
     2521 gtgcttcggt aataggaggg gttaaaagcg tatcggttgt aagagagtac tccaagcatg
     2581 cgccatcgag ggcggcatcg aaataaaatc ttgcgggggc ggcaaaagcg agagagttgc
     2641 gtgccgcgtt gtagtgcagg cgtgagactg gattggcgtg cgtcagcagt gtttctacca
     2701 aaaccgagcg cgacacaacg tcgattttga ggagataact atcccctacc ggtggccaag
     2761 ttgcgtccgt cgctgtgcgg ttgagccgcc ccagcgttgc gtagagaaat ccacccgtgt
     2821 agaaaagtgc gccaacttcg gcataaccat cggcgtcggc gtacgaggat aagtcgatat
     2881 tccctaaact gttaccgttt tgggtactca caatgccgat gctattacgg ttgaaaaagc
     2941 tcaccgcagc ttgacccgaa cccaaaaacg ctatatcttg tgggttggaa cgttcaaatg
     3001 cagtcgtgta ctcgatggtg ttgttcagac ggcgcaaaga gtctgacccc aaccgttgca
     3061 caacaaatgt cgcttgttcg gaggcgctat agcgtaatgt agaatcgttg aacaaaggta
     3121 aaatatcttt gaccacagtg tttgtcgtgg ggataagcgc gctcaaaaaa cccgttgaaa
     3181 aatcagagct cacaatgagg acaacaccgt tggcgggagc ggcatttctc tcagggcgtt
     3241 caaaatccgc acaagaaaga aaactgagcg caatcataaa aataaaaaaa taagatttta
     3301 tttcttcccg tcttaaaaga aaaagagcgt gtaatactac catgccgctg tccattttac
     3361 caagaaataa cgaccgggta gagtgtagcc aatcatatcc acttcttgat cgtcgaatat
     3421 attttttact gtaaaattaa aaacatggcg tgcgtaacta tcgagaatca aatcgacacc
     3481 cgcatcgacg agtgtttttg acgccaaata aaacgaatag ctattatatc gatctctaaa
     3541 gacagcacct ctgtattcaa cagagcccat aaatcgaaac gctcggtatg gcaccgtgat
     3601 cactcccacc gcgtggtggc gtgggcgcat cggcaagaat ttatcttggt agtaagggat
     3661 attgccataa tcgcgggcgt ctaaataagt gtatcgaaga gttaataaaa atcgctctgg
     3721 ccaattaagc aacacctcgt tttcaatgcc caaaatttgt gccctcgaag cgttcacggc
     3781 gataagcgtg cgcgatgagt tttgagtaaa aataatatag tagtgtgcgc cggttaaaaa
     3841 cgcttccgtg cgccacgagc atgaaagatt tttgaaacac ataaaactac cacgcacgcc
     3901 accggcattt gtgattgctt tctcactctt caatttttca gacggcgtga cgaacacatt
     3961 atcgccaaag cgttcataga gggtaggatt acggtacgtc gaagagactt gcgcgtagaa
     4021 ctctaaaagt gggtaggtgg ggtcattacc tggataccaa aacgctaaag ggcgcagcgc
     4081 aaaataccca gacgccccca cctcagtctc gttgaaagtt ttagcgagcg agccataaaa
     4141 cgtccccgcc tcggcacggt cgtgattcac gttgccgctt gtttgcaacg caacacgaaa
     4201 aaaagtagaa tcaaatgacg cggacccagc cccctgccac
//
LOCUS       DAOMPD010000217         4231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8708_length_4231_cov_2.197079, whole genome shotgun sequence.
ACCESSION   DAOMPD010000217 DAOMPD010000000
VERSION     DAOMPD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8708_length_4231_cov_2.197079"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLY93_08365"
     CDS             <1..410
                     /locus_tag="PLY93_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRP69530.1"
                     /translation="GRVFLGMSDGECQEGMTWEAAMAAAHNKLTNIHPFLDFNNIQID
                     GKVEHVMGVEPLDKKFTSFGWAVRVADGHDFVAIAEAFDWSLQNEGLPKMILFKTVLG
                     KGVSFMENNPDWHGTPPNQEKRDTAMVELLKAG"
     gene            complement(407..>1242)
                     /locus_tag="PLY93_08370"
     CDS             complement(407..>1242)
                     /locus_tag="PLY93_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRP69531.1"
                     /translation="TAIASLVILASLFLKLDLRALLFAEHKILTDKDETIIHALLENT
                     HIEKTKKKKTPKFLSDKDSEARGDLTKEKGFESISNDYVLSPGTRETLKSRIISAKEY
                     TRIITAKESRVHIALPREKKEETTPSTEKLRIPAYYRWRNDFALSWDYAGRPAIPTAR
                     YAHYSYFRSMINKIQSVWAPPGGDPYPTFGDSYHRMGPTSGYMRFSTYPSQDIYIQFL
                     LDENGVVRDAKLVSSLGFESLDRSCIEALFAAHDFGPPPAELMEDGTLIVPFIFRIIV
                     R"
     assembly_gap    1243..1265
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1266..2015)
                     /locus_tag="PLY93_08375"
     CDS             complement(<1266..2015)
                     /locus_tag="PLY93_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69532.1"
                     /translation="MPRNLLALFLSCLVGMLALSACGRARTLAEEGIVRLEQGRQTSA
                     LELFDRALRSNPKEPLALYGKGILLSEEQITEDIARSMLTQATQQEKLSEKYRIKAYL
                     RIAELAASRNDRDATLQNLAKISITHPAVDPQVVRHIASLYLQLKEKTRARDLLTTYR
                     ESHPKDMETEYFLFRLYTLELKDFKAAAKLCAKVNWKTAFPEKYDLNCARVLASINDF
                     PAALELVNGYLTRKGANAEKNAHDLRDSIAKK"
     gene            complement(2027..2980)
                     /locus_tag="PLY93_08380"
     CDS             complement(2027..2980)
                     /locus_tag="PLY93_08380"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRP69533.1"
                     /translation="MERQKKNLILAATLGVIFILGFLINDPFLIFETSYEKSKPVIAS
                     RLERVKKITVLDGTSKRTFTRTTDGWALDNAAGTGVLRADSEKIESGLKNLFEARRYQ
                     EVSSNKEKQGEYEVRDNDFQIQLDGERSEKIAHAYLGKYSPSGSSSFIRLAGENAVYA
                     VKGFLKGDWNQDIDQYRDHSILKIAKENIKRIEVSGKNSFALKASDTGELVLDPPRAT
                     DKARVSTYASEISEFNGQKFFREPKLPPSYGKIKITLSAGITKEIEFFGPIANQEFVV
                     KSTDMNEPLTTAKSKVDNLFPRLEDLVDKAGAPQLVVPPQK"
     gene            complement(2982..>4231)
                     /locus_tag="PLY93_08385"
     CDS             complement(2982..>4231)
                     /locus_tag="PLY93_08385"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRP69534.1"
                     /translation="SYGAKTEALSNLITLYEQQDLENQLTAKLFKMVKPNEKRIGILS
                     GHGQFNMKKERNPTSLSLFTDKISSFYGDIMEVNTSNADVPVEVSTLIIVPPEALSPI
                     ERFRLDQFLMRGGNLIVAASGMDVNFSQQFMAAPGNPDLGDFLKKYGIELNSDMVNEP
                     KPNFYIPFVQPVNAFQMSRQPYPPWVLVPKANMSQQSLVSKGNTAFILPYTSSIKTNP
                     ALLPEGEGQDKFKVDVLAKSTSDSWSQPNFAFLAPDKMEEMLAAPKQATGAYNLAVMV
                     HGRFKSQFADGKDIPKEAPKTYLKQAEKDSRILIVGTPYAFSNMMYILSEMTGAPLVE
                     ENLKLFFSAIDVMNGNEDLVSLRKKSSPAIKAKLVTDGTRKFLTLLAFVVPLLVIGIL
                     GAYRLTRRAQARLKESAPVTTEG"
BASE COUNT         1020 a          936 c         1079 g         1173 t
ORIGIN      
        1 caggccgtgt ctttctgggg atgtctgacg gcgaatgcca agagggaatg acctgggaag
       61 cagcgatggc ggcagcgcac aataagttga ccaacatcca tccgtttttg gatttcaaca
      121 acattcaaat cgacggcaag gtggagcacg tgatgggtgt cgaaccactc gataaaaaat
      181 ttacgagttt tgggtgggcg gtgcgcgtcg ccgatgggca cgattttgtc gctattgccg
      241 aagcgttcga ttggtcgctc caaaacgagg gattacctaa aatgattctt ttcaagaccg
      301 tgttgggtaa aggcgtttcg tttatggaaa ataaccccga ttggcatgga actccaccca
      361 atcaagaaaa acgcgacacg gcaatggtcg aactattgaa agccggctag cgtacaataa
      421 ttcgaaagat aaacggcaca atgagcgtgc cgtcttccat aagctcggca ggcggtgggc
      481 cgaagtcgtg cgcggcaaag agtgcttcaa tacacgaacg gtcgagcgac tcgaacccca
      541 acgaagaaac taatttggcg tcacgcacta cgccgttctc atctagtaaa aactggatat
      601 aaatatcttg gctggggtat gttgaaaagc gcatataccc cgaggtcggg cccatgcgat
      661 ggtatgaatc accgaacgtg gggtagggat caccgccggg gggagcccat acgctttgaa
      721 ttttgttaat catcgaacgg aaataactgt aatgggcata acgcgccgtc gggatggctg
      781 gcctgcccgc gtaatcccac gaaagggcaa agtcattgcg ccagcggtag tacgcaggga
      841 tacgtagttt ttcggtcgat ggagttgtct cttctttttt ttcacggggg agagcgatgt
      901 gaacgcgcga ttctttcgcg gtgatgatac gcgtgtactc tttggcggag ataatacgcg
      961 actttagagt ttcgcgcgta cccggtgaaa ggacatagtc gttagagatg gattcaaacc
     1021 ctttctcttt agttaaatcg ccgcgcgctt cggaatcttt gtctgataaa aatttgggag
     1081 tttttttctt ctttgtcttt tcaatatgcg tgttttctaa aagcgcgtgg ataatcgttt
     1141 cgtctttgtc cgttaaaatt ttatgttcgg caaaaaggag cgcgcgtaaa tcaagcttta
     1201 gaaaaagcga cgccagaata accagtgacg caatggctgt gannnnnnnn nnnnnnnnnn
     1261 nnnnnttttt tggcgatgct atcgcgtaag tcgtgcgcgt ttttttcagc attagcaccc
     1321 ttgcgagtta gatacccgtt caccagttcg agcgcagcgg gaaagtcatt tatactcgcc
     1381 aaaacccgcg cgcagttgag atcgtacttt tcgggaaagg ccgttttcca attgactttt
     1441 gcacacagtt ttgccgccgc cttaaaatct ttaagttcta acgtatagag gcggaaaaga
     1501 aaatactcgg tctccatgtc tttgggatgg ctctcgcgat acgttgtgag taagtcgcgt
     1561 gctcgagttt tttctttgag ctgtaaataa agcgatgcga tatgacgcac cacttgcgga
     1621 tccacagcgg ggtgcgtaat cgatattttg gctaaatttt gcagcgtcgc atcgcggtcg
     1681 ttgcgcgatg ccgcgagttc ggcgatacgt aaatatgctt tgatgcggta tttctcggaa
     1741 agtttttctt gttgcgtcgc ctgtgtcagc atggagcgcg cgatatcttc ggttatttgt
     1801 tcttctgaaa gaagaatccc tttgccgtag agcgcgagcg gctctttggg attgctgcgt
     1861 agcgccctgt cgaaaagctc taacgccgag gtttgccttc cctgttccaa ccggacaata
     1921 ccttcctcgg caagcgtgcg cgcacgacca cacgcgctca atgcaagcat accgacaagg
     1981 cagcttagga aaagcgcgag taaatttcgt ggcacaggct tgaaagtcac ttctgggggg
     2041 gaactactaa ttgcggcgca cccgctttat cgactaaatc ttcaaggcgg ggaaacaaat
     2101 tatcgacttt tgactttgca gtcgtcaatg gttcgttcat gtcggtcgat ttgacgacaa
     2161 actcttggtt ggcgataggc ccaaaaaatt caatttcttt tgtgattccc gcgcttaaag
     2221 taattttaat ttttccgtaa gaaggaggaa gcttgggttc gcgaaaaaat ttctgtccgt
     2281 taaattcaga aatttcagac gcgtaagtgg aaacgcgcgc tttgtcggtg gcgcgcggtg
     2341 gatcgagcac gagctcaccc gtatcgctgg ctttgagagc aaagctattt ttccctgaaa
     2401 cttcaatccg cttaatattt tctttggcga tttttaagat agaatggtcg cggtattggt
     2461 cgatgtcttg attccagtcg cctttcaaga aacctttcac cgcatagacg gcgttttcac
     2521 ccgcaaggcg gatgaaactg gaactccccg aaggagagta ttttcctaaa tacgcgtgtg
     2581 caatcttttc agagcgttcg ccgtctaact ggatttgaaa atcgttgtcg cgcacttcgt
     2641 attcgccttg cttctctttg tttgaagaaa cttcttggta gcgacgcgct tcaaaaagat
     2701 tttttaatcc cgactctatt ttttcgctgt cggcacgcaa aacgccggtg cccgcagcgt
     2761 tatctaacgc ccagccgtcg gtggtgcgtg taaaagtacg cttcgatgta ccgtcgagaa
     2821 ctgtgatttt tttaacgcgt tcgagtcgcg atgcaatcac cggttttgat ttttcgtaac
     2881 tggtttcaaa aatcaaaaat gggtcgttga ttaagaaacc taaaataaaa atcacgccaa
     2941 gcgttgccgc taaaattaaa ttcttttttt gtctttccat gttaaccttc cgttgttacg
     3001 ggtgcgctct ctttgagccg cgcttgggcg cgccgtgtga ggcggtatgc gcctaaaata
     3061 ccgatgacga gtagagggac tacaaaagcg aggagagtta aaaatttacg tgtgccgtcg
     3121 gttacaagtt tagctttgat ggccggtgac gatttttttc gtaaactgac taaatcttcg
     3181 ttgccgttca taacgtcgat agccgagaag aaaagtttta agttttcttc tacaagcggc
     3241 gcgcctgtca tttcgcttaa aatatacatc atgttgctaa acgcatacgg agtgcccacg
     3301 attaaaatac ggctatcttt ttcggcttgt ttgagatagg tcttgggcgc ttctttaggt
     3361 atgtctttgc catcggcaaa ttggctttta aagcgaccat gcaccatcac cgctaagttg
     3421 tacgcgccgg tggcttgttt gggcgcagcc aacatctctt ccattttatc gggagcaagg
     3481 aaggcaaagt tcggctgtga ccacgagtcg ctggtggact ttgccaaaac atcaaccttg
     3541 aacttatctt ggccttcccc ctcgggtagg agcgcgggat tggttttaat actgctggtg
     3601 tacggcaaaa taaacgcagt gttgcctttg gagactaagc tctgttggct catatttgct
     3661 tttgggacca acacccacgg tgggtatggc tggcgtgaca tttggaatgc gttcaccggt
     3721 tgcacaaagg ggatatagaa attaggcttg ggttcgttca ccatatcgga attgagttcg
     3781 ataccgtatt tttttaagaa atcccccaag tcaggatttc ccggcgcagc cataaattgt
     3841 tggctgaagt tgacgtccat ccccgatgcc gccacgatga gattaccacc gcgcatcaaa
     3901 aattggtcta aacggaaacg ttcgatagga gaaagcgctt cgggtggaac gataattaag
     3961 gtagaaactt ccactggcac atcggcgttg ctggtgttca cttccatgat gtcgccgtag
     4021 aacgaagaaa ttttgtcggt gaagagcgaa agcgaagtgg gattgcgctc tttcttcatg
     4081 ttaaactggc cgtggcctga caaaatacca atccgctttt cattgggttt aaccatttta
     4141 aaaagtttgg cggtgagttg attttccaag tcttgttgtt cgtaaagcgt aatgagatta
     4201 ctcaacgcct cggtttttgc gccgtaagat a
//
LOCUS       DAOMPD010000218         4220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8728_length_4220_cov_2.123409, whole genome shotgun sequence.
ACCESSION   DAOMPD010000218 DAOMPD010000000
VERSION     DAOMPD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8728_length_4220_cov_2.123409"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLY93_08390"
     CDS             complement(<1..1161)
                     /locus_tag="PLY93_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP69535.1"
                     /translation="MEFERTTDNLIKNLRVYITDKPGYLGKVLTKVGMHGANIGDLRL
                     IKRAAKNTLREIEIYVRDQEQLQEIVHAVGELDGIQVASVTDAVLDLHKGGKIQMVSR
                     VPVDTPSDIRRIYTPGVAAVCRAIQENRELMYDYTYIQNTVAIITNGTAVLGLGDIGV
                     EAGMPVMEGKAVLFSKLVGINGIPILVDSKDIDTCVNTIVSIAHSFGAIKLEDFRAPE
                     CFAIEAALKEKLEMPVMHDDQHGTATVVLAALLNATKYVGDDLRALSVGIIGLGAAGT
                     GIASLLKAFGVQKIYGADIDASAGERLTQMGGTPLPLAELMQSAQVVVATTGVPNLIK
                     PEMIKSRQIILALSNPDPEIKPDVAVEAGAAFAADGRGVNNALAFPGIFKGAL"
     gene            1243..1947
                     /locus_tag="PLY93_08395"
     CDS             1243..1947
                     /locus_tag="PLY93_08395"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRP69536.1"
                     /translation="MVKLVLIRHGQSLWNLHNLFTGWVDVPLSSQGVAEAIAAGKRLA
                     EEKFNVIYTSTLIRAITTAMIVMSENKEGKVPILQKSKSDVPANWPAQAAPFENWVKI
                     YSSTAEANSIPVYSAWELNERMYGELQGMNKEETAQKFGDAQVKIWRRSYDVPPPAGE
                     ALKNTAERTIPWFQKNIEPQLKGGKNVLVAAHGNSLRAIIMYLEKLTEAQVLELELKT
                     GDPIIFRAEGTTITRA"
     assembly_gap    2113..2122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2169..2663
                     /locus_tag="PLY93_08400"
     CDS             2169..2663
                     /locus_tag="PLY93_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRP69537.1"
                     /translation="MGLKFLGIDPGYARMGYGLIEVNGNRATFVDAGVLETKASDAEG
                     ARLTQLQHFLKKYLAKHKVTHAGIEQVFLRRDLTTGIRLAEARGVIVMSLFQAGIPYS
                     EISPSAMKKSITGSGRAEKKSMQLMTAKILNLPTQMKIDDAADGLGLAFSAWLKYQAI
                     LLTR"
     gene            2686..>3052
                     /locus_tag="PLY93_08405"
     CDS             2686..>3052
                     /locus_tag="PLY93_08405"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69538.1"
                     /translation="MNARMESIEKKIVTLENEIKALRTEFEDATRAETKNTPAGVPPQ
                     IHPTPPPRVSAKSHSSWEWLVGGNLIGKLGIVTLILSAAFFMVYALDKGWLNPWTRIA
                     LLEIASAAAAYLSYRLYKKN"
     assembly_gap    3053..3062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3063..>4220
                     /locus_tag="PLY93_08410"
     CDS             <3063..>4220
                     /locus_tag="PLY93_08410"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69539.1"
                     /translation="RYVPEALAIVALPASVIAIYSAHFIYHYIERIPTMLLMLGLMSL
                     TLFYARRIRAQALTSVLFVGFFALPVIHSHNINETAAYFTYLFAVNILYTALVYFSLL
                     RREHTLSPHTLWSVAAGNALSLLGWAQKFSEYSIIPLLFCAVTFTLLLWSAHQSQWST
                     DERSDKVLRNLAVFFVNLLYTVMTPTVLNLNKNFHSDIFALAYLALAALNVTAVDFLA
                     LRTRSIAAAATSVVLLLTLAMVIFLHGGAERLALAVFLTAVMYMSARWPDKLVYYVTA
                     YLNLLNLLVVFGSVQSNADNVFLLNWQAATLFTYAAGATYLLTRKLWPSAFNFGTLLA
                     ITALLTSFVGIIAELNRLFQDSDTRLLIATLLLAVYALVLLTLGFKRHSVA"
BASE COUNT          999 a         1136 c         1025 g         1040 t
ORIGIN      
        1 gagcgcacct ttgaaaatgc cggggaacgc caacgcgtta ttcacgccgc gcccgtcggc
       61 ggcaaacgct gcccccgctt cgacggcaac atcgggcttg atttcaggat cggggttgga
      121 aagcgctaaa ataatttggc ggcttttaat catttcgggt ttgattaaat tcggcacgcc
      181 ggtcgtcgcc accacaactt gcgcactttg cataagctcg gccagcggca acggggtgcc
      241 tcccatttgc gtcagccttt ccccagccga tgcgtcgatg tcggcgccgt aaattttctg
      301 tacgccaaac gctttgagta acgaagcaat ccccgtgccc gccgcaccta aaccgatgat
      361 gcccaccgac agcgcgcgca aatcgtcgcc cacatatttt gtggcgttca agagcgcggc
      421 caaaacgacc gtcgcggtgc cgtgttggtc gtcgtgcata accggcatct ccaatttttc
      481 tttgagtgcc gcttcaattg caaagcattc aggtgcacgg aaatcttcga gcttaatagc
      541 accgaacgaa tgcgcgatgc tcacgatggt attcacacag gtgtcgatgt cttttgagtc
      601 gacaagaatc ggtatgccgt tgatgcccac gagttttgaa aaaagaacag cctttccttc
      661 cataaccggc atgcccgcct cgacaccgat gtcgcccaag cccaagacag cggtaccatt
      721 ggtgatgatg gcaacggtgt tttgaatata cgtgtaatcg tacatgagtt cgcggttttc
      781 ctgaatcgca cggcacaccg cagccacacc aggagtgtag atgcggcgga tatcactggg
      841 ggtgtcgacg ggcacgcggc tcaccatctg aattttgccg cctttatgca agtcgagcac
      901 cgcatcggtg accgacgcca cctgaatgcc gtcgagctca cccactgcgt gtacaatttc
      961 ttgcaactgt tcttggtcgc gcacgtagat ttcaatttca cgcaatgtat ttttcgccgc
     1021 acgtttgatg agccgcaaat cgccaatgtt cgcgccgtgc atccccactt tggtgagcac
     1081 ttttcccaaa tacccgggtt tgtcggtgat atagactcgt agatttttaa ttagattatc
     1141 ggtggtgcgt tcaaattcca tacgggcgga ttttcaacgg tgaataggga gtaatcccct
     1201 ttcaacttct ttacggcacc tctcgacacc acacttgcgc ccatggtaaa actagtttta
     1261 atccgccacg gccaatcgct gtggaaccta cacaatttat tcaccggctg ggtcgatgtg
     1321 ccgctgtcga gtcagggtgt ggccgaagcg attgccgcag gcaagcgcct cgctgaggaa
     1381 aaattcaacg tcatctatac atccacttta attcgagcaa tcaccaccgc gatgattgtc
     1441 atgtctgaaa ataaagaagg taaggtgcct attttgcaaa aatcgaagtc ggatgtcccc
     1501 gccaactggc cggcgcaggc tgcgcctttt gaaaactggg tgaagattta ttcgtccact
     1561 gccgaggcca attccatccc tgtttactct gcgtgggagc tcaacgagcg catgtacggc
     1621 gagttgcaag gcatgaataa ggaagaaacc gcgcagaaat tcggcgacgc gcaggtaaaa
     1681 atttggcgac gcagctacga cgtacctccg cccgccggtg aagctctcaa aaataccgcc
     1741 gagcgcacca taccgtggtt tcaaaaaaat atcgaaccgc aactcaaagg cggtaaaaat
     1801 gtactcgtcg ccgcgcatgg caacagcttg cgggcaatca ttatgtattt agaaaagttg
     1861 accgaagcgc aagtgttgga actcgaactg aaaaccggcg atccgattat tttccgcgcc
     1921 gaaggtacta caatcacacg cgcgtagaga aagtgttagg tgaactcgcg cttttaattt
     1981 tacaacattt gttcgacaga caaggcaagc gcgttgccat cgtcgattta gaagcggtga
     2041 cgggtaaaga catccacaca ttgcgtgccg ccatcgaaga cttaaaaatg caaagccttg
     2101 tcgccgaaga cgnnnnnnnn nnacagcatt gaaattcgac ccgaggggat tgcattcgca
     2161 cgcagccgat gggtttaaaa tttttaggaa ttgatccggg ctatgcacgg atgggctatg
     2221 gtctcattga agtcaacggc aaccgcgcca cgtttgtcga tgcaggagtt ttagaaacaa
     2281 aggctagcga cgccgagggg gcgcggctta cccaactgca acacttcctc aaaaaatatt
     2341 tagctaaaca caaagtcacg cacgcgggta tcgagcaagt ttttttgcgg cgcgacctca
     2401 ccactggaat ccgtctcgcc gaggcgcgtg gcgtcatcgt gatgagtctt ttccaagcgg
     2461 gaatacccta ttctgaaatc tcgcccagcg caatgaaaaa atcgatcacc ggttcgggca
     2521 gagccgagaa aaaatcgatg caactgatga cagcaaaaat cttaaatttg cccacacaaa
     2581 tgaaaatcga cgacgccgcc gacggcctgg gtctcgcgtt cagtgcatgg ctcaaatacc
     2641 aggcgatatt attgactcgg tgaaacgctc tgcctaaact gccgcatgaa tgcgcgcatg
     2701 gaaagcatcg aaaaaaaaat tgtaacgctt gaaaatgaaa tcaaagcact gcgtaccgaa
     2761 tttgaagacg caacgcgcgc tgaaaccaag aacactccgg cgggcgttcc tcctcaaata
     2821 caccccacac ctcccccccg cgtaagcgcc aaatctcact catcgtggga atggctcgtg
     2881 ggtggcaacc tcatcggcaa actcggcatc gtgactctta tactctcggc ggcgttcttt
     2941 atggtgtacg cgctcgacaa aggttggctt aacccgtgga cgcgcatcgc tcttttagag
     3001 atcgcctcgg cagcggcggc gtatttgagt taccgcctct acaaaaagaa ttnnnnnnnn
     3061 nnccgctacg tacccgaagc gttagcgatt gtggcgttgc cggcgagtgt tatcgccatc
     3121 tacagcgcgc attttattta ccactacatc gagcgcatac caacgatgct gctcatgctc
     3181 ggcttaatgt cgctcactct tttttacgca cggcgtatcc gcgcgcaggc attaacgtcc
     3241 gttctctttg tgggattttt tgctctaccc gtaatccatt cacataacat caatgaaacc
     3301 gccgcgtatt tcacttattt gtttgcagtg aatatacttt acaccgccct cgtctatttt
     3361 tcgcttctgc gccgcgaaca tacactctca ccgcacacgc tatggagcgt ggcggccggc
     3421 aacgcgctga gtcttttggg gtgggctcaa aagttttcag aatattcgat tataccattg
     3481 cttttttgtg cggtaacgtt tacgctgcta ttatggagtg cccaccaatc gcagtggagt
     3541 accgacgagc gttccgataa agttttgcgt aacctcgccg ttttttttgt gaaccttctt
     3601 tatactgtga tgacgcccac cgtgctcaac ctcaataaaa atttccatag tgatattttt
     3661 gcacttgcct acttggcgtt agctgcgctc aacgtcaccg ccgttgattt tttagccttg
     3721 cgtacgcgtt caattgcggc ggcagcgacg agcgtggtgc ttttactaac tttggctatg
     3781 gttatttttc tccacggcgg cgcagaacgt ttagctctcg ccgttttttt aaccgccgtc
     3841 atgtacatga gcgcacgatg gccggacaaa ctcgtttact acgtaactgc gtatctcaat
     3901 ttactcaatc ttctggtggt attcggttcg gtgcaaagca acgccgataa cgtgtttctc
     3961 ttgaactggc aggccgccac acttttcacc tacgccgccg gtgcaacgta cttgctcact
     4021 cgaaaacttt ggccgtcggc atttaatttc ggcacacttc ttgccatcac tgctctactc
     4081 acgtcgtttg ttgggatcat cgcagaactc aaccgccttt ttcaagatag cgacacaagg
     4141 ttgctcatcg cgacattgct gttggcagtg tatgcgttgg tgctattgac gttgggcttt
     4201 aagcggcata gcgtcgcaat
//
LOCUS       DAOMPD010000219         4193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8799_length_4193_cov_2.877477, whole genome shotgun sequence.
ACCESSION   DAOMPD010000219 DAOMPD010000000
VERSION     DAOMPD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8799_length_4193_cov_2.877477"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..>2514
                     /locus_tag="PLY93_08415"
     CDS             237..>2514
                     /locus_tag="PLY93_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69540.1"
                     /translation="MPWRTKNVFRITTSLPARLIDSIMAQGNWNLLFQESPFVDFQHT
                     LAVANFFAMRDDTRQAEYYFEKARNNLAITYASLRADYGWPKNFHEIKNAQSLPWGES
                     RDAFEDFLLCRFQLSIELLLAGMNNLGEEKDKLAAEINALETQLGKTLRQKDASFAAA
                     MMFIQHSFALRQNETDKNLLAARFAQMSDAQKNSISDYWQQRLLGFKIQENIRYGNLG
                     RALFLIRFIEENKKEKVDAVALVRLYIRCGAFSDAERLLTNTLASPEIRFPDNFNNYT
                     VVSQLYENLMAWRGEFKRGETQTQIAYEHITSLLMHEDTLKEDFVDMRKAAHNERLRQ
                     FIFAYIDRSACPSRNDFVDDSDMEREWLFKQAIFFERCKIALDKAVWAPLVQSMTNDE
                     KQILAYHRREELVQKNQSTIAVFINARFQLRKVLKRKNKKSLPFFLTKYLKAQNAVHP
                     IFFTLDWGLYFSDNLVYEALQELPGAITKKEAWQIFTELNRTFAWQKLSHGFFTIFEP
                     PEASLISQKANQFILDVLGDAAIPDSQNNNAHALYFTDGKIALFYEPQGQKGKLWHGE
                     AHTFKENLKADDIVFFGDALNLQNKLSKKIRAFLPALFFQCDTCKNIKAAPRFVASTT
                     STWASELSDNFSVVWNFERERDCLLGESKYSDTLLLINTTSPPELPCNLTVENIIIDV
                     QDKPSVFQSLGVLSLGYRERSALISMPRKMEAQNKIAFLFDLFQKTNRRQIPIHPAFE
                     EAKQRAEKSFSTNGEFSTL"
     assembly_gap    2515..2524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2525..2798
                     /locus_tag="PLY93_08420"
     CDS             <2525..2798
                     /locus_tag="PLY93_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69541.1"
                     /translation="MSRYTKIICFALLSVVACVQPAPAIDKTVVDQTLQDFTAERISY
                     YLHDARPKTNREFLDIVLSRRGISIRSFAPELKRYYPNEYKQLMGE"
     gene            complement(2847..3368)
                     /locus_tag="PLY93_08425"
     CDS             complement(2847..3368)
                     /locus_tag="PLY93_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRP69542.1"
                     /translation="MHNDGSFHRWQDALTEQNIHLLESNTSPLALPTGVVIDAQNKWT
                     LIESLCTALCTREKLDATISSAIHKELIAREKLMSTGIGEQMAIPHAVIPEACRFMTE
                     CVIIKNGLDFDSIDTHKAYIVVLLVAPKAALQEHLQVMASIARVFYKVEARTQFIAAR
                     TATDALQIVRAHA"
     gene            complement(3458..3793)
                     /locus_tag="PLY93_08430"
     CDS             complement(3458..3793)
                     /locus_tag="PLY93_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP69543.1"
                     /translation="MDIKLHEKQGYYVVYLKGSLDMYTSVELKSQTDSIALKNGDKII
                     LQLNDVSYIDSSGIGVLIKIVNSVQAAGGAVHLTGIKPMIEKIFKVAGLMSFFAFITE
                     EEFKSKYPV"
     gene            3857..>4193
                     /locus_tag="PLY93_08435"
     CDS             3857..>4193
                     /locus_tag="PLY93_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69544.1"
                     /translation="MRRQAFILFPIVALLWVAPILAQMNGTGDLSHPTVDELHKKLNA
                     LVARFKSDADERRKNAQKNEGVKKGKQPMDENCPADSFCIVLARGGMRTALDRDNPVV
                     FEEIIMKMNG"
BASE COUNT         1185 a          927 c          921 g         1150 t
ORIGIN      
        1 cgtcttcagc atctttgaag gcgccgaaga agtgctggcg ttgcgcgtga ttgcgcgttc
       61 tcttttagcg cgcgcggcgg ggctcaagag cgcataacgt aacacggttg tttgcgcttc
      121 ataaaaactc gttacgtcgc aagcgacaag cgcggtctaa gcgtccgcgc ttttttattt
      181 tggtaagccc aattcttctc tttattttgc tcactttagt cacggcgacg gacgctgtgc
      241 cgtggcgtac caaaaatgtt ttccgcataa ccacatcgct acccgcgcgc ctcatcgact
      301 cgattatggc acagggcaat tggaatcttc ttttccaaga aagcccattc gtggattttc
      361 aacatactct cgccgtggcc aatttcttcg cgatgcgcga cgatacaagg caggcggagt
      421 attatttcga aaaagcacgc aataacttag cgattacata cgcatcgtta cgcgccgatt
      481 acggttggcc taaaaatttc catgaaatta aaaacgcaca aagcttaccg tggggtgaat
      541 cgcgggatgc gtttgaggat tttcttttat gccgtttcca gttgagtatc gaacttttgc
      601 tcgctggtat gaataatctc ggtgaagaaa aagataaact tgccgctgaa ataaacgctt
      661 tggaaacgca attaggaaaa acattgcgtc aaaaggacgc gtcatttgcg gcagcgatga
      721 tgtttattca acatagcttt gcattacgcc aaaatgaaac ggataaaaat cttttagccg
      781 cgcgttttgc tcaaatgagc gacgcgcaaa aaaattcaat cagcgactat tggcaacagc
      841 ggttactcgg ttttaaaatt caagagaata tccgttatgg aaatttaggt cgtgctcttt
      901 ttctcatccg ctttatcgaa gaaaataaaa aagaaaaagt cgacgcagtg gctcttgtgc
      961 ggctttatat ccgttgcggc gcgttctcag atgccgaacg gctcttgaca aatacgcttg
     1021 catcgccaga aataagattc cccgacaatt tcaataacta caccgttgta agtcagctct
     1081 acgaaaactt gatggcatgg cgtggtgagt ttaagcgcgg cgaaacgcag acgcagattg
     1141 catacgaaca catcacatcg cttttgatgc acgaagatac cttgaaagaa gattttgtcg
     1201 atatgcgtaa agcagcacac aacgagcgtt tgcgacagtt tatctttgcc tacattgacc
     1261 gtagcgcatg cccgtcgcgg aacgattttg tcgacgacag cgacatggag cgcgagtggc
     1321 tttttaagca ggcaattttt ttcgaacgtt gtaaaattgc gctcgataaa gcggtttggg
     1381 cgccgcttgt tcaatcgatg acaaacgatg aaaaacaaat tttagcgtac caccgtcgtg
     1441 aggaattagt acaaaaaaat caaagtacga ttgctgtttt tattaacgca cgattccaat
     1501 taagaaaagt gttaaagaga aaaaataaaa aatctcttcc cttttttttg acaaagtatt
     1561 tgaaagcaca gaatgcggtg catcccattt tttttacgct cgattggggg ctgtacttct
     1621 cggataattt agtctatgag gcgttacaag aactacctgg agcgataaca aaaaaggagg
     1681 cttggcagat ttttacagaa ctcaaccgca cttttgcatg gcagaaatta agccatggtt
     1741 tttttacgat ttttgaaccc cccgaagcaa gcttgatctc acaaaaagcg aatcaattca
     1801 ttcttgatgt tctcggcgac gcagcaatac cagactctca aaacaataat gcccatgctc
     1861 tctattttac cgacggcaaa attgcattgt tttacgaacc acaaggacaa aagggcaaac
     1921 tgtggcatgg cgaggcgcat acgtttaaag aaaatttaaa agccgacgat attgtttttt
     1981 ttggggacgc tcttaatctg caaaacaaat tatctaaaaa aattcgcgct ttcttacctg
     2041 cgcttttttt tcagtgcgac acttgcaaaa atataaaggc agcgccgcgc tttgtcgcat
     2101 caacgacatc gacgtgggca tccgaattga gcgataattt ttcggtggtg tggaattttg
     2161 agagggagag agattgtctt ttgggggaga gtaaatacag cgatacgctc ctcttgataa
     2221 acacgacatc tcctccagaa ctaccgtgca acttaaccgt ggaaaacata attatcgacg
     2281 tacaagataa accttctgtt ttccaaagtt taggcgttct ttcgctgggc tacagagagc
     2341 gctcggcact catctcaatg ccaaggaaga tggaagctca aaataaaatt gcctttttgt
     2401 tcgatctatt ccaaaaaaca aatcgacggc aaataccaat acaccccgcc tttgaagaag
     2461 caaagcaaag agcggaaaag tcgttttcaa caaacggcga attttcaacc ctgtnnnnnn
     2521 nnnntatgag tcgttacact aaaattattt gttttgctct cttgagtgtt gtggcatgtg
     2581 ttcaaccagc gcccgccatt gacaaaaccg ttgtggatca aactttgcaa gattttacgg
     2641 cagaacggat tagttattat ctacacgatg cgcggcctaa aacaaaccgt gaatttttag
     2701 acattgttct ttcgcgacga ggtatttcaa tccgtagttt tgcgcccgag cttaagcggt
     2761 actacccaaa tgagtacaaa cagttgatgg gcgagtagcc tctcatcgcg taacttaaac
     2821 actagcaggt tgccgagaaa accttgctaa gcgtgcgcgc gcactatctg tagcgcatcg
     2881 gtagcagtgc gggcggcgat gaattgagtc cgcgcctcca ctttgtaaaa cacgcgtgca
     2941 atcgacgcca tgacttgcaa gtgctcttgt aacgccgcct ttggggcaac gagtagcacc
     3001 acgatatatg ctttatgcgt atcgatactg tcaaagtcaa gcccgttttt aatgatgacg
     3061 cattctgtca taaaacggca tgcctcggga atgactgcgt ggggtatcgc catttgctcg
     3121 ccgatccccg tactcattaa tttttcgcgc gcaatgagtt ccttgtgtat tgcgctcgag
     3181 atagttgcat cgagtttttc acgcgtacac aaagccgtac acaagctctc aatgagtgtc
     3241 catttatttt gggcgtcgat cacaaccccc gtcggcagag caaggggcga ggtatttgac
     3301 tcaagcaggt ggatgttctg ctcggtcaat gcatcttgcc accggtgaaa acttccatcg
     3361 ttatgcacat gtattctatt acacgaaagt tataggcggt caagcttatt cataataggg
     3421 tatgccgcag agttaaaatt gagagagatg actcttatta tacagggtac tttgacttaa
     3481 attcttcttc ggtaataaaa gcaaaaaagc tcatgagacc tgccactttg aatatttttt
     3541 caatcattgg ctttataccc gtcaaatgga cggcgccacc tgcggcttgt accgagttga
     3601 cgattttaat gagaacaccg atgccgcttg aatcaatgta gctaacgtcg tttagttgta
     3661 aaataatctt atctccattt ttcaatgcaa tgctatcggt ttggctttta agctcgaccg
     3721 acgtatacat atcaagactc cctttcaagt aaacaacata atatccttgt ttttcatgta
     3781 gttttatatc cattttttac acctaattag agtttacgct atatgtaagc acgtatcacg
     3841 tcgataatat attactatga gacgccaagc atttatttta tttccgatcg ttgccctttt
     3901 atgggttgcc ccaattttag cgcaaatgaa tggtaccgga gatttaagcc atcccactgt
     3961 cgacgagctg cataaaaaac tcaatgcact tgttgcccgt ttcaaaagcg acgccgatga
     4021 acgacgtaaa aacgcacaaa aaaacgaagg cgtcaaaaaa gggaaacagc caatggatga
     4081 aaattgcccc gccgattcgt tttgcatcgt gttggcgcgc ggtggtatgc gcaccgcgtt
     4141 agaccgagac aatcccgtgg tgtttgaaga aatcatcatg aaaatgaatg gca
//
LOCUS       DAOMPD010000220         4182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8839_length_4182_cov_3.033923, whole genome shotgun sequence.
ACCESSION   DAOMPD010000220 DAOMPD010000000
VERSION     DAOMPD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8839_length_4182_cov_3.033923"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLY93_08440"
     CDS             <1..315
                     /locus_tag="PLY93_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP69545.1"
                     /translation="RKAKLQLKQAERRALIEIEDARVAFEKTTAEREAYAKSAEAAEK
                     NYRAQLNDLRTSIATVLDVIQALQDLQNARNALFNAEYQEKISRLRYFVALGYLFPES
                     KF"
     gene            complement(325..858)
                     /locus_tag="PLY93_08445"
     CDS             complement(325..858)
                     /locus_tag="PLY93_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP69546.1"
                     /translation="MDAAKTLFADKGFEGTNVRDIIHESGLSPGTFYNYFQSKEEIFE
                     VLTDEIIDEVRAEIQQSYKDVKMEESEIRTSLKKLFQVFLGNPRLMKFLSRNQSYLRE
                     LRSKGRFNAMLADLEKALEDGIKNGMLPQLPVKIVSIAVFGAVFEIIATMVITPNFAV
                     DKAIETLSQLLFIQTQK"
     gene            954..1580
                     /gene="pdxH"
                     /locus_tag="PLY93_08450"
     CDS             954..1580
                     /gene="pdxH"
                     /locus_tag="PLY93_08450"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRP69547.1"
                     /translation="MDLHSVRMEYTGDDISQLPIEPYRAVELWLAEALKVEDDPTAFA
                     LATHSNERLSVRIVLAKNIDQNGVVFYTNGVSSKGQQIEAKAQAAGVFFWPKLHRQLR
                     FEGKVTHLAESDADAYFESRPHDSRVAAYVSHQSQSIESYKKLYEDFEKAKKNFAEKS
                     IPRPPTWHGYTIAFECVEFWQGQPNRLHQRLLYTRTENDTYRREWLQP"
     gene            complement(1591..2721)
                     /locus_tag="PLY93_08455"
     CDS             complement(1591..2721)
                     /locus_tag="PLY93_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69548.1"
                     /translation="MPQAIEDGYYLSLGAGANQLPLIRAARAHGLKVIAVDQNMDALG
                     FKESDLRIQESIFNYRKIFYKIVKIVDPSQIVGGYAASYGSALLSLAFLCERLHLIGL
                     SRTLVETVQDKLQVRRLVSSIEHNSFAQPLFVEIVQALRREEIEQLGFPLIAKIRRGA
                     SKKHIYKLENWGEVKNFLSRRNLDDLGIRGSDLEQYIEGDEIIVTGFSQNFNFNLVSI
                     HDRIAAKEPPYIDLEHRFPSKYAHLAGAIRDVQNQVCIKLQLADTPVVSEWKVVAGRL
                     YLVEFSAQIPGEHVADFMIPKGLGYDYFKNLVRLTLGEKVESAPEKHKQNITIKFWPQ
                     NPGSSEWQEAIQRAAFARVLNEKNSKKIASNLDRYAVAGFIN"
     gene            complement(2744..3541)
                     /locus_tag="PLY93_08460"
     CDS             complement(2744..3541)
                     /locus_tag="PLY93_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69549.1"
                     /translation="MTIFLFSLSTATQINAAEDTKFDLSSDQLENANTQESVVYGNTL
                     ENMRHAEKPRFVTLVDTFEYARGTGLQTRGVLFSYHNRAAREVRFIVDIPGFPPQAMR
                     RNKYGVWTYFYAPPEIVHTSPQKKVRYKFWVDGLYTADDTHTQHEKDSTGQIASVFHL
                     LHDQFAAATGAFVLDTPVNHGREVLFRIPAGDAETVSLAGDFNRWNPERDFLTKSEDG
                     YFELRKVIAPGKYTFLYRIDGKLAQKVATKSSRIHPVYGRVNFLEIK"
     gene            3624..>4182
                     /locus_tag="PLY93_08465"
     CDS             3624..>4182
                     /locus_tag="PLY93_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69550.1"
                     /translation="MADEQPNPEQEVVSYTPEELQTIQELIEFFNNAPGQVTVDVDQN
                     VDISDENPPEVDDALDLSDDVPDVSKRPVADLSKFDDIMNMDVDNFDAPPEQPTEIQD
                     VPLVNDAPLDGMGDIPLAQSDGSDLDTLPDLGATDMGDADALPDLAATDMGNADALPD
                     LAATDMGNADALPDLAATDMGNADAL"
BASE COUNT         1139 a          985 c          964 g         1094 t
ORIGIN      
        1 cgtaaagcaa aacttcaatt aaaacaagcg gaacgtcgtg cgcttattga aattgaagat
       61 gcgcgcgtcg cgtttgaaaa aacaaccgcc gaaagagaag cgtacgcgaa atcggcagaa
      121 gccgccgaaa aaaattaccg cgctcaatta aacgatttgc gcacttccat cgccaccgtg
      181 ctcgatgtaa tccaagcatt gcaagattta caaaatgcgc gtaatgccct ctttaacgca
      241 gagtatcaag aaaaaatatc gcggctgcgt tattttgtcg cgttaggtta tttattcccc
      301 gaatcgaaat tttaagagta tttgctattt ttgcgtttga ataaaaagaa gctgcgaaag
      361 cgtttcaatt gctttatcca cagcgaagtt gggggtaatc accatcgtgg cgatgatttc
      421 aaaaacggcg ccaaaaacag cgatggacac aatttttact ggtaactgtg gcaacatgcc
      481 gttttttata ccgtcttcaa gtgctttttc taaatcggca agcatggcgt tgaagcggcc
      541 cttgctgcgc agttcgcgca gataagattg gttgcgggaa agaaatttca tgaggcgcgg
      601 attgcccaag aatacttgaa ataatttctt taaggatgtg cggatttcac tctcttccat
      661 ttttacgtct ttgtacgatt gttgaatttc agcgcggact tcatcgataa tttcgtcggt
      721 taaaacctca aaaatttctt ctttactttg gaaatagttg taaaaagtcc caggggacaa
      781 tccgctttcg tgaataatgt cgcgtacgtt tgtaccctca aagcctttgt cggcaaaaag
      841 ggtttttgca gcgtccaaaa tttgaaaccg attttggagt tttgtttgcg cgttccacct
      901 ttgccaactg tttttttgcg gacggcctgt atataaaaag cgaacagagt ctcatggatt
      961 tacatagtgt gcgtatggaa tatacaggcg atgatatatc acaattacct atagagccat
     1021 accgtgccgt tgagctttgg ctcgccgagg cgttaaaagt cgaagacgac cccaccgcct
     1081 ttgcgctcgc tacgcacagc aacgagcgcc tttcggtgcg tattgtgctt gcgaagaata
     1141 tcgaccaaaa tggagttgtg ttttacacca atggcgtgag ctctaaaggc caacagattg
     1201 aagcaaaggc gcaagcggcg ggcgtgttct tctggcctaa actacaccgg caactgcgct
     1261 tcgaggggaa agtgacgcat ctagccgaaa gcgatgccga tgcttacttt gaaagccgtc
     1321 cgcacgactc gcgtgtggct gcgtacgttt cacaccaaag ccaaagtatc gaatcgtaca
     1381 aaaaactcta tgaagacttt gaaaaagcaa agaaaaattt tgccgagaaa agcatcccac
     1441 gaccgccaac ttggcatggc tacacgattg cttttgagtg cgtggaattt tggcagggac
     1501 aacccaatcg gctgcaccaa cgattacttt acacgcgcac cgaaaatgat acatatcgac
     1561 gcgagtggtt gcaaccttga attttaacga ttagttaata aaacccgcca cagcgtagcg
     1621 gtctaaattc gacgcaatct ttttggaatt tttttcattg agcacgcgag cgaaagcggc
     1681 acgttggatg gcttcctgcc actcgctcga tcctgggttc tgaggccaaa atttaatcgt
     1741 aatgttttgt ttgtgttttt cgggcgcaga ctcaactttt tctcccaatg ttagccgaac
     1801 gagattctta aagtaatcgt agcccaatcc tttgggaatc ataaagtcgg ctacatgctc
     1861 tccaggaatc tgcgccgaaa attcaacaag ataaagtcta cctgcaacaa ctttccattc
     1921 gctcaccacg ggcgtgtctg caagttgtag ctttatgcac acttgatttt gcacgtcgcg
     1981 gattgccccc gcaaggtgag cgtactttga aggaaaccta tgttcgaggt cgatgtacgg
     2041 tggttctttc gcggcgatgc ggtcgtgaat agaaactaaa ttaaaattaa aattctgaga
     2101 aaaaccggta actattattt catcgccttc gatatactgc tctaaatcgc tcccccgaat
     2161 ccccaaatcg tctaaattgc ggcggcttaa aaaatttttc acctcacccc agttttctaa
     2221 tttataaatg tgcttcttcg atgcaccccg tcgaattttt gcaatgagcg gaaagcccaa
     2281 ctgctcgatc tcctcgcgcc gaagcgcttg cacaatttcg acaaaaagcg gttgtgcaaa
     2341 tgagttgtgc tcaatgcttg aaacgagccg ccgcacttgc agtttgtctt gaactgtctc
     2401 caccaatgtg cggcttagac caataaggtg cagccgttcg caaagaaatg ccaaactcaa
     2461 tagcgccgaa ccgtagcttg ccgcatagcc accgacaatt tgtgacggat ctacaatttt
     2521 aacaattttg tagaaaattt ttcgataatt aaatatggat tcttgaatgc gtaaatcgct
     2581 ttctttgaaa ccaagagcat ccatattttg atcgacggca atgactttaa ggccgtgagc
     2641 gcgcgccgca cgaatgagtg gtaattggtt tgcacccgca cctaatgaga gataataacc
     2701 gtcttcgatc gcctgaggca tcagttaaat atcggcattt aacttacttt atctctaaaa
     2761 aattcacgcg gccgtacacc ggatgaatgc ggctggattt tgtcgcgact ttttgcgcca
     2821 gcttcccgtc gattcgatag agaaacgtgt acttccccgg cgcaataact ttacgcagtt
     2881 caaaataacc gtcttcgctt ttggtgagaa aatcgcgctc ggggttccaa cggttaaaat
     2941 cgccggcaag gctcacggtt tcggcgtcgc cggcggggat acggaacaag acttcgcgac
     3001 catggttcac cggcgtgtcg agaacaaagg caccggttgc agctgcaaat tgatcgtgca
     3061 ataaatgaaa caccgaggca atttgtcctg ttgaatcttt ttcgtgttgc gtgtgcgtgt
     3121 cgtcggcggt gtaaagtcca tcgacccaaa atttatagcg tacttttttc tggggggaag
     3181 tatgaacgat ctcaggcggg gcgtaaaaat acgtccatac gccatattta ttgcgccgca
     3241 tcgcttgagg gggaaatccg ggtatgtcga caataaagcg cacttcgcgc gctgctcggt
     3301 tgtggtatga aaaaagaacg ccacgagttt gaagtcctgt gccgcgtgcg tattcaaacg
     3361 tgtcgaccag cgtcacaaac cgaggttttt cggcatggcg catattttct aacgtattgc
     3421 cgtacacaac agactcttgt gtattcgcat tctccagttg atccgacgat aaatcaaatt
     3481 ttgtatcttc ggcggcattt atctgtgtcg ccgtgctcaa tgaaaaaaga aatatggtca
     3541 taaatacttt gtgcgacata aaaccctcaa attattttcg gaaaaaccga taaatacttg
     3601 agagattcta tcttaagcgc gctatggcag acgagcaacc aaaccccgaa caagaagtag
     3661 ttagctacac tcctgaagag ttgcaaacga ttcaagagtt aatagaattt tttaataacg
     3721 cgcctgggca agttaccgtc gatgtggatc agaatgtaga catttccgat gaaaatccac
     3781 ccgaggtcga tgacgcgctc gatttatccg acgatgtacc cgacgtctcg aaacgaccgg
     3841 ttgccgattt atctaaattt gacgatatca tgaacatgga cgtcgataat ttcgatgccc
     3901 cgccagaaca acccactgaa atacaagatg ttcctcttgt aaacgacgct cctttagatg
     3961 gcatggggga tatccccctt gctcaaagcg atggcagtga tttagataca ctccccgact
     4021 tgggcgcaac agacatgggg gacgccgatg cactccccga cttggccgca acagacatgg
     4081 ggaatgccga tgcactcccc gacttggccg caacagacat ggggaatgcc gatgcactcc
     4141 ccgacttggc cgcaacagac atggggaatg ccgatgcact cc
//
LOCUS       DAOMPD010000221         4176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8854_length_4176_cov_2.287794, whole genome shotgun sequence.
ACCESSION   DAOMPD010000221 DAOMPD010000000
VERSION     DAOMPD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8854_length_4176_cov_2.287794"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLY93_08470"
     CDS             complement(<1..304)
                     /locus_tag="PLY93_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRP69551.1"
                     /translation="MKKILILIAALAFIACGKKPANEAAPAQATAQAGAPNGEALYND
                     KACATCHGAQGHGDGPVGAALKPKPRNFADAKWKFGTDLKSVIHTIENGSPGTGMAP"
     gene            455..1150
                     /locus_tag="PLY93_08475"
     CDS             455..1150
                     /locus_tag="PLY93_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRP69552.1"
                     /translation="MLLWSEIILRLFLASLLGAIIGLERERKDWAAGMRTHMLVCLGS
                     ALAMLVSTYGFNDVLKNEHIALDPSRVAAQVISGIGFIGAGTILFLKDGVVRGLTTAS
                     GLWTVAAIGLAAGGGMYFAAAVATALGLVILWVLAPIERRLSERLKGKNVRIVMSNAM
                     SPSQILSTLVVKNKTPVSHFSFEKEGDNYIVDVRFHDESPTKWIKAIEIIKREKAVKE
                     VVWNHAQYKTVRS"
     gene            complement(1147..1845)
                     /gene="rsmI"
                     /locus_tag="PLY93_08480"
     CDS             complement(1147..1845)
                     /gene="rsmI"
                     /locus_tag="PLY93_08480"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRP69553.1"
                     /translation="MPTRTGTLYSVALPIGNSADITERAKAVLASVDFIACEDTRKAL
                     DLFRRAAIATKARLLVHNPFHEAQSAEGLLKLLRDGQNVALISDAGTPRLSDPGSRVL
                     RLAFMEKIPVVPVPGAAALTALLSVTPFSIDPFLFLGFLSPKSTRRQNELKKYQEFEG
                     AVALYESVHRIEKCIVDIQSVFPHAEILIGRELTKTHEELFWGTTAEAAAWVKGKHGE
                     FALIFRKNKLNGTH"
     gene            complement(1845..3044)
                     /locus_tag="PLY93_08485"
     CDS             complement(1845..3044)
                     /locus_tag="PLY93_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRP69554.1"
                     /translation="MFINQRPFSFFFILAFVSLYSSMTCLRNEKTLKSAYAQKQSEPI
                     FTTNTDGETVALANTAPASQPTQDTALPLTPKSQREKRVNPTDAKSLLTFIEKLQHHK
                     FSWAHFPLKRPNFVKGIYLTNATASNPKSFKHFVDRSKEYKLNAMVIDTQGRKLTTEQ
                     MSYIKSQGIYPIARVVCFDLGLKTQVPNTVHLDKIMASVEEAAQMGFPEIQLDYIRYA
                     DEPKLLRLPLKFKYEQISKVLDRARKLTDSLGVELGADVFGRITLNQHDQIGQRLEVF
                     GEYVQNLYPMVYPSHYYGDPEKIADPYGTVKEGVANSKERIPKTRIIAWIQGFAMKIK
                     ESGLALPEYVLQQFYAIDDAGGDGYIVWNARNDYSATWKALKEYERLAPTRKKAHVQK
                     ERIDKQG"
     gene            3152..3856
                     /gene="ubiE"
                     /locus_tag="PLY93_08490"
     CDS             3152..3856
                     /gene="ubiE"
                     /locus_tag="PLY93_08490"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRP69555.1"
                     /translation="MYENVEKRATVRGMFNRIAPTYDLLNHLLSFGIDNLWRKATIKK
                     MALRSDSVVLDLACGTGDLSNEAVKKGVRTILGVDPSQQMLRRAAAKLQYNATRCYFI
                     EAFGEELPLKNNLCSHAMIAFGIRNVSERHRAFTEIFRVLKRGGIFTILEFTPMERKI
                     PAFFFNLYFQKILPFVGGIISRDKEAYKYLPESVARFVTAQDLIHECESVGFTTLEKR
                     AFSMGVATCILLQKPV"
     gene            complement(3860..>4176)
                     /locus_tag="PLY93_08495"
     CDS             complement(3860..>4176)
                     /locus_tag="PLY93_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRP69556.1"
                     /translation="RTPKLRIEGQKDKIAERMKYAGHVKLDKTPFLELFMGIYSVLII
                     YTAFAFNRTMFVPFMVIYAAGFFYIGIASLYENFKEMRAKREFSLTTATAAAIVAQQI
                     KK"
BASE COUNT          970 a          893 c         1114 g         1199 t
ORIGIN      
        1 agggagccat gccagttcct gggctaccgt tttcaatagt gtggattacc gatttgaggt
       61 ctgtaccaaa tttccattta gcgtcggcaa agttacgcgg ttttggttta agcgccgcgc
      121 ccactggacc atcgccgtga ccttgtgcgc cgtggcaggt tgcgcacgct ttgtcgttgt
      181 aaagcgcttc accgttggga gcaccagctt gtgctgtagc ttgtgcaggc gctgcttcgt
      241 tggctggttt tttgccgcaa gcaataaagg ccaatgctgc aattaaaata agaatttttt
      301 tcatttcgag tctccttaaa agacggctaa aacttacgtt ttgccatagg gctgactttt
      361 cttttgaaat acgatgtcga tttatttttg acaattccat gacaaagttg acactttttt
      421 tgcttttgta aacgagtatt catatatcgc agtcgtgctt ttatggtctg aaattatatt
      481 acgccttttt cttgcttctt tgttaggcgc aattatcggt cttgaacgag aacgaaaaga
      541 ttgggcggca gggatgcgta cgcatatgct ggtgtgtttg gggtcggcgc tggcgatgct
      601 tgtttcgacg tatggcttta acgatgtgtt gaaaaacgaa catatcgctc tcgacccatc
      661 gcgtgtggcg gcgcaagtca ttagcggcat cggttttatc ggcgcaggca caattttatt
      721 tttgaaagac ggagttgtgc gtggattgac caccgcttcg gggttgtgga ctgtggcggc
      781 aatcggtctt gctgccggtg gcggtatgta ttttgctgcg gcggttgcaa ccgcattggg
      841 acttgttatt ttgtgggtgc tggctcccat cgaaagacgg ctttccgaaa gactgaaagg
      901 gaagaatgta agaattgtca tgagtaacgc aatgagcccc tctcaaatac tctcaaccct
      961 ggtggtaaaa aataaaactc ccgtctcgca tttttctttt gaaaaagaag gggataacta
     1021 tatcgtggat gtccgcttcc acgatgaaag tccaacaaaa tggatcaaag caattgagat
     1081 tataaagcga gaaaaagcgg tgaaggaagt ggtatggaac cacgcgcaat acaaaacggt
     1141 gcgcagctaa tgagtcccgt tgagtttatt tttacgaaaa atgagcgcaa actcgccgtg
     1201 ctttcctttt acccaagcgg cggcttcggc agttgtgccc caaaataatt cctcgtgcgt
     1261 ttttgtcagt tcgcgtccaa ttaaaatctc cgcgtgggga aagacactct ggatgtcgac
     1321 gatgcatttc tcaatccggt gtaccgattc ataaagagca actgctccct caaactcctg
     1381 gtattttttg agctcgtttt gtcgccgtgt gcttttcggc gaaagaaaac ctaaaaacaa
     1441 aaaggggtca atcgaaaatg gggtcacgct caaaagtgca gtgagcgccg ccgcgcccgg
     1501 aacaggcaca acggggattt tttccataaa agcaaggcgt aagacacgcg agccggggtc
     1561 ggagaggcgg ggggtgcctg catcggagat aagcgccacg ttttggccat cccgcaaaag
     1621 tttcagcagt ccttctgcgc tttgtgcctc gtggaaaggg ttgtgcacta aaagccgtgc
     1681 ttttgtggct attgctgctc tacgaaataa atccaacgct ttgcgcgtgt cttcgcaagc
     1741 gataaagtca acgctcgcaa gcaccgcttt ggcgcgctcg gtgatgtcgg cgctgttgcc
     1801 aatggggagc gcgaccgaat agagcgtgcc tgttcgtgtg ggcatcagcc ttgtttgtcg
     1861 atgcgctctt tttggacgtg tgcttttttg cgcgtgggcg ccaagcgttc gtattctttg
     1921 agtgctttcc atgtcgcaga ataatcgttg cgcgcattcc acacaatgta gccgtcgcct
     1981 ccggcatcgt cgatggcgta aaactgttgg aggacgtact cgggtaaagc gagtcccgac
     2041 tctttgattt tcatcgcaaa gccttggatc catgcgatga tgcgtgtttt agggatgcgc
     2101 tctttggagt tagcgacgcc ttctttgact gtgccgtaag gatcggcaat tttttcagga
     2161 tcgccgtagt agtgcgaagg atacaccatg gggtataagt tctgtacgta ctcgccaaag
     2221 acttcgaggc gttggccgat ttggtcgtgc tggttcaagg taatccgccc aaaaacatct
     2281 gcgcccaatt caacgcccaa cgaatcggtg agctttctgg ctcggtcgag gactttgcta
     2341 atttgttcgt atttgaactt gagcggtagg cgcagaagtt tgggttcgtc ggcgtagcgg
     2401 atgtagtcga gttgaatttc agggaagccc atttgggcgg cttcttcaac gcttgccata
     2461 attttgtcca agtgcacggt gttggggact tgggttttaa gacctaaatc aaaacagaca
     2521 acccgcgcga taggatagat gccttgactt ttgatatacg acatttgttc ggtggtgagt
     2581 tttctgcctt gagtgtcgat caccatggcg ttaagtttat attctttgct acggtcgaca
     2641 aaatgcttga acgacttggg attggacgcc gttgcattgg tgaggtatat ccctttcaca
     2701 aaattaggac gcttgagtgg gaagtgtgcc catgagaact tgtggtgttg tagtttctct
     2761 ataaaagtta agagtgattt tgcgtctgtg ggatttaccc gcttctcacg ttggctcttt
     2821 ggggttagtg gtaaagcggt gtcttgggtg ggttgagaag cgggagcggt attggcgagg
     2881 gcgactgttt caccgtcggt gttggtggta aagatgggtt cgctttgttt ttgcgcatac
     2941 gcacttttga gtgtcttttc attgcgtagg catgtcatgc tgctatagag agagacaaaa
     3001 gcaagtataa agaaaaaaga aaacgggcgc tggtttataa acatagttat cttcattttc
     3061 ggcatggcgt ttgggttcat ccccattttt tttgtaagac atgccgacaa cgaatgacga
     3121 gacgttgaac ttttcgatgt tgccctaacg catgtatgaa aatgttgaga agcgcgccac
     3181 cgtgcgtggt atgtttaacc gtatcgcccc cacttacgat ctactcaacc atttgttgtc
     3241 gtttggtatc gataatttgt ggcgcaaggc cactatcaaa aaaatggcgt tgcgctccga
     3301 ttcggtggtg ctcgatttag cgtgcggcac cggcgacttg tcgaatgaag cggtcaaaaa
     3361 aggcgtacgc acaattttag gcgtcgaccc atcgcaacag atgttgcgcc gtgccgcagc
     3421 aaaactgcaa tacaatgcaa cgcgctgtta ctttatcgaa gcgttcggcg aagagttgcc
     3481 gcttaaaaat aatctttgct cgcacgctat gatcgctttt ggcatccgca acgtttccga
     3541 acgccaccgt gcttttacag aaatttttcg ggttttgaaa cggggtggga ttttcacaat
     3601 tttggagttt actcccatgg agagaaaaat ccccgccttc ttttttaatc tttatttcca
     3661 gaaaatttta cctttcgtcg ggggtatcat tagccgcgac aaagaagcgt acaagtattt
     3721 acccgaatcg gtggcgcgtt ttgtcaccgc gcaagattta atccacgaat gcgaaagtgt
     3781 tggttttacg acgttggaaa agcgcgcttt ctctatgggg gtggcgacgt gtattctttt
     3841 acaaaagcct gtttagcagt tattttttta tctgctgggc aacgatagcg gctgcagttg
     3901 cggtagttaa tgaaaactct cgtttcgccc gcatttcttt gaagttttcg taaagagaag
     3961 cgataccaat ataaaaaaat ccagcagcgt agatgaccat aaaaggtaca aacatggtgc
     4021 ggttgaacgc gaaagcagtg tagataatga ggacactata aatccccata aagagttcga
     4081 ggaacggtgt cttgtcgagc ttgacgtggc ctgcatattt catgcgttcg gcaatcttgt
     4141 ctttctggcc ttcgatacgt agtttgggag tgcgga
//
LOCUS       DAOMPD010000222         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8860_length_4172_cov_2.678650, whole genome shotgun sequence.
ACCESSION   DAOMPD010000222 DAOMPD010000000
VERSION     DAOMPD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8860_length_4172_cov_2.678650"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLY93_08500"
     CDS             <1..258
                     /locus_tag="PLY93_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRP69557.1"
                     /translation="PLDAETILTETKDAKLVVTFEEHNTLAGLGGAVCELLSENNPKR
                     VHRIGLKDEFGQSGTIEALMQHYKLTAETMLPELVAFARAL"
     gene            complement(455..>1666)
                     /locus_tag="PLY93_08505"
     CDS             complement(455..>1666)
                     /locus_tag="PLY93_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HRP69558.1"
                     /translation="EGISATWIMLGWISGDLFTWFWVPKALRRLSAKQKNTTIPEFLA
                     YDKSNGPSTSIRILVALLTAVFLGIYAAAQLHAGSKAIHVLFGFEEYIGILAGSLIVL
                     LYSFSGGIRASIYTDSVQAVVMLASMLLLVTLSLTKLGGFTELFTSLAAIDPKLVRWN
                     KEGLKFGLFLYALGWLFAGIGVTGQPHIMIRAMTIDNVKNFKTARNVYFTWYVLFTIA
                     TLIVGLSCRVLLPELYASPELALPKLSYKLLPEFATGIVLAGLVSATISTADSQILSC
                     AAAISQDATCTKRETIGSTRLATAVAIVIETVIALSATSTVFNLVAISWSVLASALSP
                     LMILRALEVSLPNAVSILTMVTGLSTMLIWRFIFGLQNSVFDAMPGIIMGFIPFLIYR
                     LYRFKACAPTR"
     assembly_gap    1667..1676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1864..2682)
                     /locus_tag="PLY93_08510"
     CDS             complement(1864..2682)
                     /locus_tag="PLY93_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRP69559.1"
                     /translation="MNTTQRTREYYNSSDADEFYYRIWGGEDIHIGIYADINEPIRDA
                     SRRTVATMASMVNITSLSRVLDLGSGYGGAARYIADKIGCNVVCLNLSATENKRNIEK
                     NKEANLDKKIEVVEGSFEQLEFGDATFDIVWSEDAILHAGDKKRVFAEIARVLKPGGF
                     LIFTDPMQADNCPEGVLEPVLQRIHLQSMGSIKLYRELAKENKFREIEIRPYHDQLTL
                     HYTRVRDELLRKKEELLRYVSLEYIERMLAGLTHWVESAAKNYLAWGILHFEKM"
     gene            complement(2679..3560)
                     /locus_tag="PLY93_08515"
     CDS             complement(2679..3560)
                     /locus_tag="PLY93_08515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP69560.1"
                     /translation="MPHFFNFGHETMNDLQNFKNHPTEIRETNHYQQEYISQFVQKWD
                     ELINWEARSKSEGDFFIRSLKKFDAKRILDVATGTGYHSVMLLNAGFEVTSVDGNPEM
                     LARAFTNARKHGHILKTIHADWRWLNRDVHEKYDAIICLGNSFTHLFSERERRKALAE
                     FNAALRHNGVLIIDQRNYDSIIDQGYNSKHAYYYLGQNVKAEPEYVDAGLARFKYEFL
                     DGSIFHLNMYPLRRQYMRRLLLEVGFQRVVTYGDFQASFDKDDPDFLVHIAEKKFIEN
                     EPELESEDNSKIEREDD"
     assembly_gap    3685..3694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3695..>4172
                     /locus_tag="PLY93_08520"
     CDS             <3695..>4172
                     /locus_tag="PLY93_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRP69561.1"
                     /translation="DLDVGKKTARLVDIQTNEQNSLTRATLLELKAILDETLKDEKYA
                     SLVFTSTNPKFFSNGLDAANVMKTPDDKLAEEVGEIVLFFNHLLAFDKPVITEVTGYA
                     MGGGAVITTGSDYKYMLEGKGRISFSEVLVGLPLPVNFVEKIQRSVYPPYLNEVCAG"
BASE COUNT         1165 a          932 c          910 g         1145 t
ORIGIN      
        1 ccattggatg cagagaccat cctcaccgaa accaaagatg caaagctggt tgtaaccttc
       61 gaagagcaca acacattagc agggttgggc ggcgcggtgt gcgaactttt aagcgaaaac
      121 aaccccaagc gcgtccaccg tattggcctt aaagatgaat ttggccagtc gggcacgatt
      181 gaggcattga tgcagcacta taaactcacc gctgaaacca tgttgccaga actggtggcg
      241 tttgctcgcg cgctttgaat tttctctaac aggctgtagc aggcgttgaa gccgtgcata
      301 ggtgtgccgg tcacgttgac atgttcgctg aaagtgactt ctacgctaat cgtttgtccg
      361 gcactccatg taccgtcggc ctcacggcgt tgcacgccgc ttttgtggaa gaatttgtgt
      421 aaaatagcgt acaaaaattt gcaaaaaaat tgtctcagcg agtgggcgca caggctttaa
      481 atcgatacaa gcgataaata agaaaaggga taaaccccat tattataccc ggcatagcgt
      541 caaaaactga attttgtaat ccaaaaataa atcgccagat taacattgtc gataatcctg
      601 taaccatagt aagtatactt accgcgttgg gtaaagagac ttccaaagcg cgtagtatca
      661 taagcggcga aagagcgctt gccaacaccg accaagaaat ggcaacaaga ttgaagacgg
      721 tacttgtcgc cgataaggca attacggttt ctatcacgat tgctaccgcc gtggcaagac
      781 gtgtagagcc tattgtttct ctttttgtgc aagtagcatc ctgtgatatc gctgcggcac
      841 acgaaagaat ttgggagtca gcagtcgata ttgttgcaga gaccaaccct gcaaggacga
      901 tacctgtagc aaactcgggt aatagtttat acgataattt gggcaacgcc aattcggggc
      961 tcgcgtatag ttcgggtaac aagacgcggc aacttaaccc tacaataagc gtagcgatag
     1021 taaaaagaac gtaccaggta aaataaacat tccgcgccgt tttaaaattt tttacgttgt
     1081 cgatcgtcat ggcccgaatc ataatatggg gttgcccggt aactcctata cctgcaaaaa
     1141 gccaaccaag tgcatagaga aaaaggccaa acttcaaccc ctctttgttc cacctaacta
     1201 atttagggtc aatggccgct aaacttgtaa ataactctgt aaatcctccc agtttggtaa
     1261 gactgagcgt taccagaaga agcattgaag ctaacatcac aaccgcttgc actgaatcgg
     1321 tatagattga cgctcgaata ccgccggaga aagaataaag aaggacaatg agagagccag
     1381 ctaaaatacc gatgtattct tcaaaaccaa aaagaacgtg tattgcttta ctaccagcgt
     1441 gtagctgtgc ggcggcataa ataccgagaa atactgccgt taataacgct actaaaatac
     1501 gaatactcgt cgatgggccg tttgatttat cgtatgctaa aaattcggga attgtcgtgt
     1561 ttttttgttt ggccgaaagg cggcgcagag ctttgggaac ccaaaaccaa gtgaataagt
     1621 cgccggaaat ccaacctaac ataatccatg tggcagaaat tccttcnnnn nnnnnngtgg
     1681 cgccgatgag gcctataaac ataaaaccgc tattattcgt cgacacggct gaaagcgcaa
     1741 cgctccacgg attcacgttt ctgcccgcta ataaataatc gtttttattg tttttggctt
     1801 tgtgggcggc gtaaacaccg atacccatta ctaagaccat aatcagagta aaggttatcg
     1861 tcattacatt ttttcaaaat gcagaatgcc ccacgctaga tagttttttg cagcggactc
     1921 gacccaatgc gttaacccag cgagcattcg ttctatgtat tcgaggctaa catacctaag
     1981 tagctcttct ttttttcgca acagttcgtc gcgtacccga gtatagtgca atgttaactg
     2041 atcgtgatag ggacgaattt cgatttcacg aaacttattt tctttagcca gttcgcggta
     2101 tagttttatg gagcccatcg attgcaaatg gatgcgctgt aaaacgggtt ctaagactcc
     2161 ctcaggacag ttgtctgctt gcatgggatc tgtaaagatg agaaagccgc ctggttttaa
     2221 tacgcgcgca atttctgcaa aaacacgttt tttgtcgcca gcgtgtaaaa tagcgtcttc
     2281 agaccacaca atgtcaaaag tagcgtctcc aaattcaagt tgctcgaaag aaccttcgac
     2341 aacctcaatt tttttatcaa ggttagcttc tttatttttt tcgatattgc gtttattttc
     2401 tgtcgcgctt aggtttaagc agacgacatt acaacctatt ttatcggcga tatagcgtgc
     2461 tgcgccgcca tatccggagc ctaaatctaa tacacgcgat agagaggtta tattaaccat
     2521 cgatgccatt gtcgcgacag ttcgtcggct agcatcgcgg attggttcgt ttatatcggc
     2581 atagataccg atatgaatat cttcgccacc ccatattcga taataaaatt cgtcggcatc
     2641 agaactgttg taatattctc gagtcctttg cgtcgtattc aatcgtcttc cctttcaatt
     2701 tttgaattat cttcgctttc cagttcgggt tcattttcga taaatttttt ctcggcgata
     2761 tgaactaaaa aatcgggatc gtctttatca aaagatgcct gaaaatcacc gtacgtgacg
     2821 accctttgaa aaccgacctc gagcaataag cgacgcatat attgtctacg tagcggatac
     2881 atgtttaaat gaaaaatact accgtctaaa aattcatatt tgaatcgtgc caatcctgcg
     2941 tcaacgtact ctggctcagc tttgacattt tgacctaaat aatagtaggc atgttttgag
     3001 ttatagcctt ggtcgatgat tgagtcgtaa ttgcgctgat cgataatcaa tacgccatta
     3061 tggcgtaacg cagcgttaaa ttcggctagc gctttgcgac gctcacgttc tgagaagaga
     3121 tgagtaaatg aatttcctaa acaaataatg gcatcgtatt tttcatggac atctcgattg
     3181 agccatcgcc aatctgcatg tattgttttt aaaatatgac cgtgtttgcg tgcgttcgta
     3241 aacgctcttg ccaacatttc aggattaccg tcgacgctcg ttacctcgaa gcccgcgttt
     3301 aacaacatca ccgaatggta gccggtgcct gttgcaacat cgagaatgcg tttagcgtcg
     3361 aattttttta aacttcgtat aaaaaagtcg ccttcgcttt ttgagcgcgc ttcccagtta
     3421 ataagctcat cccatttttg gacaaattga ctgatatatt cttgttgata gtggttggtt
     3481 tctcgaattt ctgtggggtg gtttttaaag ttttgtaaat cattcatcgt ttcgtgacca
     3541 aagttaaaaa agtgaggcat cgtgtacagg gttttgccat tatgcgagtg ggcgcacagg
     3601 ctcacaggcg ctgaggcata tatttactct gctttacccc ctgactttga cataaaataa
     3661 gcgcataatg aattttaaac tatcnnnnnn nnnnccgatt tggacgtcgg taaaaaaacg
     3721 gcgcgcttgg tcgatattca aaccaacgaa caaaactcgc tcactcgggc gactctctta
     3781 gaactcaaag cgattctcga cgaaacattg aaagacgaga aatacgcttc gcttgtattt
     3841 acatccacca accccaagtt tttctccaac ggcctcgacg cggccaacgt gatgaaaacc
     3901 cccgacgaca aactcgccga ggaagtgggt gagattgtcc ttttcttcaa tcacttatta
     3961 gcgtttgata aaccggtcat caccgaggtc accggctacg cgatgggcgg cggtgcggta
     4021 atcaccaccg gcagcgatta caaatatatg ctcgagggca aaggccgcat ttcgtttagc
     4081 gaagtgttgg tgggcttgcc tttgcccgtt aactttgtgg agaaaattca acgcagtgta
     4141 tatccccctt atctaaatga ggtgtgcgcg gg
//
LOCUS       DAOMPD010000223         4169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8867_length_4169_cov_2.822314, whole genome shotgun sequence.
ACCESSION   DAOMPD010000223 DAOMPD010000000
VERSION     DAOMPD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8867_length_4169_cov_2.822314"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="PLY93_08525"
     CDS             complement(<1..1888)
                     /locus_tag="PLY93_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRP69562.1"
                     /translation="MEIRRYRLFFVLFIALATTIGCAGFGDPGLGDGIERFSVFYSEP
                     GRDQYTMVDKRVDDELVKLIDGAERYAYLAVYNFTKDSVVQAVLRAYKRNIDMRVVGD
                     IDEFYTSGYQAMYFNNINMTLGNATGIQHDKFAVVDDKYVFMGTGNISDTDMVRNNNN
                     WYILENPNIVKLYKDEFLQMHNGLFASAKRQRNATNTFVMNNHPMEVYFSPYQGDQAM
                     DRIIALVESATTSVHYMIFAYTHDELDAAMVKMARQKNIPVYGIHDSTFIRGVSEEAP
                     KLYSAGFNNDGTMNPTGPFVRWDGNENTSVKNNPAHGGKMHCKTVLIDAGTDHARMAT
                     GSFNWSNNAINNNDENVIIVNQPRVVNAVYEQWKGAWGIAYDLALKVPVRGHTASLND
                     IIISEVGWAGSSNGSTIDNGDDFIEIFNNTSQPIDLSHWAVQWGNQDSRNLYPVPDDT
                     NWYYENKYSCGGPLRPNLICPYETRLFYNKSLSAFAVTGAAEQIIYDEYGGEIRNSTG
                     GSLSTNHFKFSGTKNFKLSNSQFKIRLYDKAMNLIDEAGDGGTALGGKYGDYESPPKT
                     YSMERRGFDGTKFTKHQLGSTSAAWFTAFAPYNCTSRQVGSVFDGCTDKAANTYSSAG
                     YIYST"
     gene            2047..>4169
                     /locus_tag="PLY93_08530"
     CDS             2047..>4169
                     /locus_tag="PLY93_08530"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP69563.1"
                     /translation="MLFGMNCSGSTYSQFFEKYDLNFSSYDGVQIAGNPTYTPTGTYT
                     GGTTGYIDITWSAPVYGDAAKTTGLDSAALTALFTQNSGNSTAIGIACITDTASVACP
                     GIQPSVGATQMRVHFTHTGVTSGVETIQLKAALDTIYDEKGAALPTSAGSAVIPLPDR
                     WAPTVVNVTASTPNGAYKAGDTISIQVVFSENVTVTGIPQLTLATGTPATTAVSYVST
                     SPANTLNFTYTIASGNNSADLDYATTAALTAGASIKDAAGNNATLTLATPGSAASLGA
                     NKAIVVDTTAPAAPAALALLAADDTGVSNSDGITNKTSGMTIEGAAEANSTVKVYSPN
                     TGGTLLGTVTADGVGNWSLAGLSFTETTHTIVATATDGAGNVSVASSVLSVTVDTTAD
                     AAPTALDLINASDTGASNSDNITKTTAPSFTVSCVNGSSVQLFDGATVTGSAVTCVGG
                     TASLTTAVLADGAHSITAKQTDTAGNVSAASAALSVTIDTTAPTGYSVAIDQSYINAG
                     NETNVSFTVAGAELGISFNYSISSDGGGAPLTGSGIVGTASFQITALNVSTLGDGNLT
                     VGLTLTDTAGNAGLLVTNTKIKDKVAPVISAVTPTTGLGVTSSAVGYTLSEDCSTASA
                     VWTHTGGSADGTSPHTRTFTGAELNSGAHSNIAFGSNPLVDLAVYSITFSCTDVAGNA
                     ATAVTVTGVTYSDGPPTIQSALALDT"
BASE COUNT          958 a         1113 c         1090 g         1008 t
ORIGIN      
        1 ccgtcgaata aatataccct gccgaggagt aggtgttggc tgccttgtcg gtgcagccgt
       61 cgaaaacaga ccctacctga cggctggtgc agttgtaagg ggcaaatgca gtaaaccatg
      121 cggcggatgt actacctaat tgatgctttg taaattttgt gccatcaaaa cctcgccgtt
      181 ccatcgagta ggttttgggt ggggattcat agtcgccgta tttccccccc agtgcggtgc
      241 cgccgtcacc cgcctcatcg atgaggttca tcgctttgtc gtaaagacga attttgaact
      301 gtgagtttga aagtttgaaa ttcttggtgc cggaaaattt gaaatggttg gtcgacagcg
      361 accctccggt cgagttgcga atttcaccgc cgtattcgtc gtaaataatt tgctccgcag
      421 ctccggttac ggcaaacgcc gaaagggatt tattatagaa aagacgggtc tcgtagggac
      481 aaatgaggtt ggggcgcaac gggccaccgc atgagtattt attctcgtag taccagttgg
      541 tgtcgtcggg caccgggtat aaattgcgcg agtcttggtt gccccactgc accgcccagt
      601 gcgataaatc gatgggttga gaagtgttat taaaaatttc aataaaatcg tcgccgttgt
      661 cgatggtgct tccgtttgac gaacccgccc agcccacttc gctaatgata atatcgttga
      721 gggaggcggt gtggccgcgc acaggtacct tgagagccaa gtcgtaggcg atcccccatg
      781 cgcctttcca ctgttcgtac accgcgttca ccacgcgcgg ttgattgacg atgatgacgt
      841 tctcgtcgtt gttgttgatg gcgttgttcg accagttgaa agaacccgtc gccatgcgcg
      901 cgtggtcggt gcccgcgtcg atgagcaccg tcttgcagtg cattttgccg ccgtgtgcgg
      961 gattgttttt taccgatgtg ttttcgttgc cgtcccagcg cacgaagggg ccggtggggt
     1021 tcatcgtgcc atcgttgtta aatcccgccg agtagagctt gggcgcttct tcggaaacac
     1081 ccctaataaa cgtcgagtcg tggataccat aaacgggaat gtttttttgc cgcgccattt
     1141 tgaccattgc cgcatcgagc tcgtcgtgtg tgtacgcaaa aatcatgtag tggacactcg
     1201 tcgtcgccga ctcgaccaat gcaataattc tatccatcgc ttggtcgcct tggtagggtg
     1261 aaaaataaac ctccatggga tggttattca tgacaaaggt gttcgtcgca ttgcgctggc
     1321 gttttgccga agcgaaaaga ccgttgtgca tttgcagaaa ttcatctttg tagagtttaa
     1381 cgatattcgg attttcgagg atataccaat tgttgttgtt gcgcaccatg tcggtgtcgg
     1441 aaatgtttcc cgtgcccatg aaaacatact tatcgtcgac caccgcgaat ttatcgtgct
     1501 ggataccggt ggcattgccc agcgtcatgt tgatgttgtt aaaatacatc gcttgataac
     1561 cgctggtgta aaactcgtcg atgtccccca cgacgcgcat gtcgatgttg cgtttatacg
     1621 cgcgcagcac cgcttgtacc accgaatctt tcgtgaagtt atagacggcg aggtatgcgt
     1681 aacgctcggc gccgtcgatg agcttgacga gttcgtcgtc aacccgctta tccaccatcg
     1741 tgtactggtc gcggccgggt tcggaataga aaacagaaaa acgctcgata ccatccccaa
     1801 gtccagggtc gccaaagccc gcacagccaa tagtcgtagc gagtgctata aaaaggacaa
     1861 agaaaagtct atagcgacgg atttccatag ctacggtgta gtaaagttgg cgaggggctt
     1921 tgtcaataac tataaaattt gtcgtactct ttgactttac gccgtctata tacgatatat
     1981 taaagaagaa acataaaagg gaacacagtg gttttacgca caaaactaat ttatcttttt
     2041 cttctcgtgc tttttggcat gaattgttca ggcagcacgt atagtcagtt ttttgaaaag
     2101 tacgacctca atttctcaag ctatgacggc gttcaaattg caggaaatcc cacttacaca
     2161 cccacgggca cctataccgg tggcacaacc ggttacatcg acatcacctg gagcgcgccg
     2221 gtctatggcg acgcggcaaa aacaaccggt ctcgatagcg ccgccctcac tgctcttttt
     2281 acacaaaaca gcggtaactc aaccgcgatt gggattgcct gcattaccga tactgcaagc
     2341 gttgcttgtc cggggataca gccttcggtg ggagccacac agatgcgggt gcattttacc
     2401 cacacaggcg tcacgtcggg tgtggaaacc atccaactga aagcggctct cgacacgatt
     2461 tacgatgaaa aaggcgcggc gctgcccaca tcggcgggtt ctgcggtgat tcctctgccc
     2521 gaccgttggg ctccaacagt cgtgaatgtt accgcttcaa cccccaatgg cgcgtacaaa
     2581 gcgggcgaca ctatttccat tcaggttgtc tttagcgaaa atgtgaccgt caccggcata
     2641 ccgcaactga cgcttgcaac agggacgccc gcgacgactg cggtgtccta tgtttcaacc
     2701 tcgcctgcaa acacactcaa ttttacctac accattgcct cgggtaacaa cagcgccgat
     2761 ttagattatg caactaccgc tgcgctcact gctggcgctt caattaaaga tgcggcgggc
     2821 aacaacgcca cgttgacctt agccacaccc ggctcggcgg cttcactcgg cgcgaataaa
     2881 gccatcgttg tcgacacaac tgcgcccgct gcgcccgccg cgctggcgct ccttgcggcc
     2941 gacgacaccg gcgtatcgaa tagcgatggt attaccaaca agacttcggg tatgacaatc
     3001 gaaggcgccg cagaggcgaa ttcaactgtt aaagtttact ctcccaacac cggcggtact
     3061 ctcttaggta cagttaccgc cgatggagtg ggtaactggt cgcttgctgg tctttctttc
     3121 accgaaacga cgcacacgat tgtggccact gcgaccgatg gagcgggtaa cgtatcggtc
     3181 gcgtcgtcgg ttctttcagt gacggttgac accaccgccg acgctgcgcc cactgcgctc
     3241 gatttaatca acgcgtcgga taccggcgca tccaatagcg ataacatcac aaaaacaacc
     3301 gcgccttctt ttacagtgag ctgtgtgaac ggctcatcgg tgcagctttt tgacggcgcc
     3361 acggtaacgg gttctgcggt aacgtgtgtg ggcggcacag cgagtctcac tacggcagtg
     3421 cttgccgacg gtgcgcatag tatcacagca aaacaaaccg acaccgcagg gaatgtgagc
     3481 gccgcttcgg ctgcgctcag cgtcacgatc gacactactg cgccaacggg ttatagcgtt
     3541 gccatcgatc aaagttacat taacgcgggc aatgaaacca acgtttcctt taccgttgcg
     3601 ggcgcagaac tgggcatatc ttttaactat tctatctctt ccgatggggg aggcgctccg
     3661 cttaccggta gcggtatcgt gggtaccgct tcgtttcaaa ttaccgcact taatgtatcc
     3721 acgctgggtg atggtaattt gacggtggga ttaaccctca ccgataccgc cgggaacgct
     3781 ggacttttgg tcacaaacac taaaattaaa gacaaagtgg cgccggtcat ttcagcggtg
     3841 actcccacaa caggtttggg tgtcacatcc agcgcagtcg gctacacact ttccgaagac
     3901 tgttcgacag caagcgcggt gtggacacac acaggcggct ctgccgatgg gacatcgcca
     3961 cacacgcgca ccttcacagg cgctgaattg aactcaggcg ctcactcaaa cattgccttt
     4021 ggtagcaacc cgttggtgga tttggcggta tactccatca ccttcagttg caccgacgtg
     4081 gctgggaatg ccgcgaccgc tgtgacagtg acgggcgtaa cgtatagcga tggtccgccc
     4141 acaatacaaa gcgctcttgc actcgacac
//
LOCUS       DAOMPD010000224         4156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8898_length_4156_cov_2.402341, whole genome shotgun sequence.
ACCESSION   DAOMPD010000224 DAOMPD010000000
VERSION     DAOMPD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8898_length_4156_cov_2.402341"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLY93_08535"
     CDS             <1..191
                     /locus_tag="PLY93_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP69564.1"
                     /translation="KKISFFDFKKSTYTGVMHVEDAAAILPDFLKKVGKQNLESFAAR
                     PLTLDDIFIKMTGRQIDV"
     gene            184..>1015
                     /locus_tag="PLY93_08540"
     CDS             184..>1015
                     /locus_tag="PLY93_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP69565.1"
                     /translation="MFRSSWQLSLMHLREFFRDPEIILWSVLLPVAMSWVLGIAFVER
                     KVTTRHVAVVGDVHQSAQLREVINEIENRNIALPKKSNDHSVFKFSFVSENEALNLLR
                     KGKIVLFIKLSPQGALHFNLDPANDTSYLAYLILSRRLSQEKVELQMNAITTRGNRYI
                     DFLLPGLMALGIMNSCLWGIGYVLIEYRMKKLMRRMIATPVKRSAILLSFFSSRIIIN
                     FVEVMLLFLFGYLYFDFTLQGSVLNILLLFVSGNVAFAGLAFLLASRAENTRTGNGII
                     N"
     assembly_gap    1016..1025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1026..1255
                     /locus_tag="PLY93_08545"
     CDS             <1026..1255
                     /locus_tag="PLY93_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP69566.1"
                     /translation="SVPLMLASGVFFNYANFPEFIQPLLQYSPLALLADSIRLVFNTA
                     GGLAEIALPLLIMNLFGVVCFSLSLRIFKWH"
     gene            1315..>2489
                     /locus_tag="PLY93_08550"
     CDS             1315..>2489
                     /locus_tag="PLY93_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRP69567.1"
                     /translation="MTRKLLEELENSENGSFELFEEKGSAILKVFNPKGDGKPVHPVD
                     LMGRIRSMQIVLDKSVSVEKILRNTQEGRLPEGNPHVIGVWPKVVVRKAATGEVHTAA
                     DHMSASIYIDPPEGGGQVLSANTIKQLLKDHGVVKGLQEEVIQNLAKKPQYKKLILVA
                     KGQAPKNGENGYIQPLFDTIHKPQRDTSAKKVDHKKVDLIRAAKIGEAIAEKINPTLG
                     IPGYTVGGGILPAEPGKIAEFQLGPNVEISPDGMKLLSKIEGRPVLDSRGRIRVDEVV
                     YLKNIDYSTGNVDFPGSVIVEESIADGFKLHASGSVILQSSVGVCDLSAGKDVILSAG
                     FMGRGEGIIRSEGDVYARFVEQGTIEAKGSIFISEAALHSKLIAGGSIVVKGGRGDVT
                     "
     assembly_gap    2490..2510
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2511..3002
                     /locus_tag="PLY93_08555"
     CDS             <2511..3002
                     /locus_tag="PLY93_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRP69568.1"
                     /translation="YLQCMKLGGAGETKTLVTIGIDPSIRENIENIKISLQEKEATLE
                     KIRISLNRLNDLMKKRALDVTESTTREKLLMAMKKYRALVDSDQRQFETAQLSLSAHP
                     KAYVSVETQIFPNVEISFGRGLIYRSPVRTNIGHQVLYVEDRAIVVQPTLPHFLQIEN
                     EES"
     assembly_gap    3100..3109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3110..4127)
                     /locus_tag="PLY93_08560"
     CDS             complement(<3110..4127)
                     /locus_tag="PLY93_08560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRP69569.1"
                     /translation="MEKPRIPFSRAPVDFESVAPLLKEAIDNGWLTTGPLVERFTNGL
                     KDYWQTQNVVTVSSCTAALQIALASLDLKRGDEVILPAMTFVATLETVEQAGLTPVLA
                     DIDPLTWNISVDAIEAKISPWTKAIIPVAFAGNPLPMQRLRSLADKHKLKLILDNAHA
                     LEAKFEGKFLHEYADFSCYSFYATKNLSAAEGGAIICAEENIERVRALSLHGMSRNAW
                     RRYAGGTWRYDIVETGFKANLTDIHAAIGIPQLEHIEKNHRRRLELIERYGDGLKGLP
                     IRFQGLETGASPIEHAAHLFTISIAPQSSVKRDEIIEAFIQHGIAYSVHFIPLCEFTS
                     VKNKY"
BASE COUNT         1068 a          885 c         1008 g         1154 t
ORIGIN      
        1 ttaaaaaaat ttcctttttt gattttaaga aatctactta cacgggggtg atgcacgtcg
       61 aggatgccgc cgctatttta cccgattttc taaaaaaagt aggcaaacaa aatttagaat
      121 cgtttgccgc acgtccttta acactcgacg atatttttat taaaatgacg ggtaggcaaa
      181 tcgatgttta gatcgtcgtg gcaactttct ttgatgcacc tacgggagtt ttttcgcgat
      241 cccgaaatta ttttgtggtc ggtgcttttg cccgtggcga tgtcgtgggt tttgggtatc
      301 gcttttgtcg agcgcaaagt gacaacgcgt catgtcgccg tggtgggcga cgtacaccaa
      361 agcgcacagc tacgcgaagt gattaacgaa attgaaaacc gcaacattgc acttcctaaa
      421 aaaagtaacg accatagtgt ttttaagttt tctttcgtga gtgaaaacga agctctcaat
      481 cttttgcgca aaggcaaaat tgtgcttttt attaaactct ctccccaagg agcgttacat
      541 tttaatttag accccgccaa cgacacttcg tatttagcgt atctcatttt atcgcggcgt
      601 ttgagccaag agaaagtgga attgcaaatg aacgctatca ccacacgcgg caatcgttac
      661 atcgattttt tactgcccgg gcttatggcg ttagggatta tgaattcgtg tctttggggc
      721 attggctatg tactcatcga gtatcggatg aaaaaactta tgcggcggat gatcgccacg
      781 cccgtaaagc gcagcgcaat tctcctttca tttttttctt cgcgaattat cattaatttt
      841 gtcgaagtga tgctcttgtt tttatttgga tatttatatt tcgattttac gttacaaggt
      901 tcggtgttga atattcttct tctctttgtt tcagggaatg ttgcctttgc tggccttgct
      961 tttcttttgg cgtcgcgcgc tgaaaacaca cgcaccggca acggcatcat caatgnnnnn
     1021 nnnnntttcg gtgcctttga tgcttgcttc gggtgtcttc tttaattatg caaactttcc
     1081 tgagtttatt caaccacttt tgcagtattc tcctttagcg cttttagccg attcgatccg
     1141 gttggtgttt aatactgcgg gaggattagc cgaaatcgct ttacctcttc ttatcatgaa
     1201 tttatttgga gtcgtttgct tttcactcag tttgcgtatt ttcaagtggc attaaagtag
     1261 tataaaaatt ttgaggattg acgatattgg taaaaacaag tggctgaaaa tgaaatgaca
     1321 cgaaagttgc tcgaggagct agagaattcc gaaaacggtt ctttcgagtt atttgaagag
     1381 aaagggagcg ctatactcaa agtctttaat cccaaagggg atggtaagcc cgtccatccg
     1441 gtggatttaa tgggacgcat ccgctcgatg caaattgttt tggataagtc ggtctctgtc
     1501 gaaaaaattt tgcgtaacac acaggaaggt cggttgccag aggggaatcc gcatgtcatt
     1561 ggagtttggc ctaaagtggt ggtgcgtaaa gcggcaacgg gcgaagtgca taccgccgcc
     1621 gatcacatgt cggcttccat ttatatcgac ccccccgaag ggggagggca agtattgtct
     1681 gccaacacga taaaacaatt actcaaagac cacggtgtgg tgaaaggctt gcaagaagag
     1741 gttatccaaa atctggctaa aaagccgcag tataaaaaat tgatcctcgt cgcaaaggga
     1801 caagcgccca agaacgggga aaatggctat atccagccgc tctttgatac catccacaag
     1861 ccgcaacgcg atacatcggc aaagaaagtc gatcataaaa aggtagattt aatccgtgcg
     1921 gcaaaaatag gcgaagcgat tgccgaaaaa ataaatccca cgttgggaat ccccggctat
     1981 accgtggggg gagggatttt acctgcagaa ccgggtaaga ttgccgaatt tcaacttggt
     2041 cccaacgtcg agatttcccc tgatggcatg aagcttcttt caaagattga ggggcgtccg
     2101 gttttagatt cgcgcggtag aattcgagtc gatgaagttg tgtatttgaa gaacatcgat
     2161 tactcaacgg gcaatgtcga tttcccgggt tctgtaattg tcgaagaatc gatcgccgac
     2221 ggtttcaagt tgcacgcctc gggtagcgtt attttacagt cgagcgtggg cgtgtgcgat
     2281 ctttcagcag gcaaagacgt tattttgtcg gcgggcttta tgggcagagg cgaaggcatc
     2341 atccgatccg agggcgatgt gtatgcgcgc tttgtagaac aaggtactat tgaagccaag
     2401 ggctccattt ttatttccga agctgcgctc cactcaaaac tcattgccgg cggctccatc
     2461 gtcgtcaaag gcgggcgggg agacgtcacn nnnnnnnnnn nnnnnnnnnn tatctgcagt
     2521 gcatgaaatt agggggagcg ggtgaaacaa aaactttggt gacgattgga attgatccat
     2581 ccatccgaga gaatatcgag aatatcaaga tctcactgca agaaaaagaa gcaacgctcg
     2641 aaaaaattcg cattagctta aatcgcttaa acgacctcat gaaaaaacgc gcgttggatg
     2701 tcacagaaag tacaacgcgc gaaaaattac ttatggcaat gaaaaaatac cgcgcgcttg
     2761 tcgattcgga tcaaaggcaa ttcgaaacag cgcagttatc gctcagcgct caccccaaag
     2821 cgtatgtatc ggttgaaact caaatttttc ccaatgttga aattagtttt gggcgcggtc
     2881 ttatttatcg aagtcctgtg cgtacgaata ttggccatca agtgctttat gtcgaggatc
     2941 gtgctattgt tgttcaaccg acattgccgc actttttaca aatagagaac gaagaatcgt
     3001 aatcacttca tcttgctctg cgttggtgag cttagggtag agcgggagtg aaagagcccc
     3061 ttgaaagtat tccatgctgt gagggaaatc agtagacttn nnnnnnnnnc gtacttattt
     3121 tttaccgatg taaattcgca cagtggtatg aaatgcacag agtaagcgat cccgtgctgg
     3181 atgaacgctt caataatttc atcgcgtttc acgctacttt ggggggctat tgatatggta
     3241 aaaagatgtg ccgcgtgctc gatgggagat gcccccgtct ctaagccttg gaagcggata
     3301 ggtagtcctt tcagaccgtc gccataccgc tcgattaatt ctaaacgacg gcgatggttc
     3361 ttttcaatat gctcaagttg cgggatgccg atcgcggcgt ggatgtcggt taaatttgct
     3421 ttgaatcccg tttcaacaat gtcgtaccgc cacgtaccgc ctgcataccg ccgccatgcg
     3481 ttgcgcgaca taccgtgtag actcaacgcg cgtaccctct cgatattctc ttcggcgcat
     3541 attatggcgc caccttcggc ggcgcttaaa ttttttgtgg cataaaaact ataacaagaa
     3601 aaatcggcgt attcgtgtaa aaacttcccc tcgaattttg cttctaaagc atgtgcgtta
     3661 tccaaaataa gttttagttt gtgtttatcc gccagacttc ggagtcgttg catcggtaac
     3721 gggttgcctg caaacgcaac gggtatgatt gcttttgtcc agggagaaat ttttgcttca
     3781 atggcgtcta cagaaatgtt ccacgttaaa gggtcgatgt cggcaagcac cggagtaagt
     3841 cccgcttgtt caaccgtttc gagtgtggca acgaacgtca tcgccggaag aatcacttcg
     3901 tcgccgcgtt ttaagtctaa cgacgcgaga gcgatttgta aagcggcagt gcacgacgag
     3961 acggtaacaa cgttttgagt ttgccaataa tctttaagac catttgtgaa acgttctaca
     4021 agcggccctg tggtgagcca gccgttgtct atcgcttctt tgagcaaggg agcgacactt
     4081 tcaaaatcaa ccggtgcgcg cgaaaagggg attcgcggtt tttccattat gagctcttgc
     4141 gtttaacggc gacacg
//
LOCUS       DAOMPD010000225         4151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8916_length_4151_cov_2.297607, whole genome shotgun sequence.
ACCESSION   DAOMPD010000225 DAOMPD010000000
VERSION     DAOMPD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8916_length_4151_cov_2.297607"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1309
                     /locus_tag="PLY93_08565"
     CDS             110..1309
                     /locus_tag="PLY93_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP69570.1"
                     /translation="MKAKIYLDFNSTHPPNKEILHRAREFYFAHFANSSGLSLASQRV
                     NKRIEEARESVAHDLSVLPQQVVFTSCATESNNLLIRAFHQRKRGGTFNVLTTRIEHP
                     SVTECIKKLPNTAIHYLECNESGGISEEELDKHDLNAYDFIAMIAVQNETGVRLPYLQ
                     LFERIPQNNPPYVLVDFSQALPKLSKDAPEDLTPAIVQRLTQKNFFLTGTGHKLGAGF
                     GAGLIVLPAMHTAWKEAGLLAGGNQEYGVRAGSHNAEAIIAFAEALHEKVQSNSYKLW
                     KNIAHHFENRLMRILNRLDVKILPENKNNNDNRAPGTTLLLLPNTPIDFLVMALDQEG
                     ITVSTGTSCKSRSRTPSAAVLAMGLSEQKALSIVRLSYSQELKEADMEYTATTFEKIL
                     TAMKDSY"
     gene            complement(1320..1829)
                     /locus_tag="PLY93_08570"
     CDS             complement(1320..1829)
                     /locus_tag="PLY93_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69571.1"
                     /translation="MEKRSIKIMNILEVHLPPIQWERIVSLAVTRKKTFSTITRYCVL
                     RLARKKNLCWNPVLITAHSKAKGGVQITDKVHRHVVCLYGEDEMLIRLAAMHLRITMS
                     AFIRLSLELFLGHLAMEKQGYNFVTDEQLKWQAIRFIERYTPHTQNCFSRPDSIHFKW
                     FNFALSSYW"
     gene            complement(2015..3628)
                     /gene="lnt"
                     /locus_tag="PLY93_08575"
     CDS             complement(2015..3628)
                     /gene="lnt"
                     /locus_tag="PLY93_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRP69572.1"
                     /translation="MGLAPFNYFPVGIVAAGVLLWASHEQHGASLRRVLVPSFIFAFS
                     LSVITFGWILPTVHRYTGAHYMLTVFLVILYTILFQTKPLFFFLLKRYFFKTNAFTYR
                     ALFFWAACAALIDAFSPELFAWSWGYTLAGSEYLRQWAAVGSVYFLSFLAFVIASIFL
                     QFFILIKKEKALVVFLKMKVGFTLILILFFVGGVLYHTPQNTAAPQLHVLTVQTNIGA
                     ADEKKRGDAAFATEAINRLFTQSTEGELLHPRVDLILWPEASMPFHSANSQKENTSIY
                     SSTFDGVLTFLSHGTGATVLYQNMTMQNGHLYSELAARPATNTTYLKRRLVPWGEYLP
                     LEKFLPSLRRVFPDAGRFHAATAAQEMIITLTENGASLPPRKELEEILPYLNSPQKIR
                     ERYGNPQHPRNVLIKPILCYEGLFPQDSHTQHASLIVNLSSDAWFGDGYAAWQHAGAA
                     SLRAVENGIPLVRAAMSGLSYAVDNRGDFIGRYSVLNTVDTLYTPIALMKRFTLFAYG
                     GMAVFYIFMGMFLFCGVFAMKFHSYGVKE"
     gene            complement(3704..>4151)
                     /locus_tag="PLY93_08580"
     CDS             complement(3704..>4151)
                     /locus_tag="PLY93_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HRP69573.1"
                     /translation="SSKDMPADERVKKYYELRRRNLGDYNSAYEERESPYTRYETEVM
                     LRSDEMQRKGTTETKTQAMREEYFGKEAAKRMAAVDTEVKEERARINTYETEAQKLIR
                     ENPSLNEVEKNKKLDELRIKTLGKEEAEEYARRMQYEEYLKKNNLK"
BASE COUNT         1197 a          955 c          885 g         1114 t
ORIGIN      
        1 agtcgcgcta aaaacattat cgccaatgcg ctaaaatcac cgtatattaa agatgaaacg
       61 atgcggcgta gtcttatgcg attgcgcttt cgtttagatt taacaaccca tgaaagcgaa
      121 aatttatctc gattttaact cgacccatcc accgaacaaa gaaattcttc accgcgcacg
      181 cgaattttat tttgcacatt tcgctaattc atcgggactc tcgctcgcat cgcaaagagt
      241 gaataaacgc atcgaagaag cgcgtgagag cgtcgctcac gatctttctg ttttaccgca
      301 acaggttgtc ttcacttcgt gcgccaccga atccaataac ttgctcatcc gcgcgtttca
      361 tcaaagaaag cgcggcggaa ctttcaacgt cctcaccaca cgcatcgaac atccctcggt
      421 caccgagtgt atcaaaaaat tgcccaacac tgcaattcat tatctcgagt gcaatgaatc
      481 gggaggcatt tctgaagaag aactggataa gcacgattta aacgcgtatg atttcatcgc
      541 aatgattgcc gtgcaaaatg aaaccggagt cagattgcct tacctgcagc ttttcgaacg
      601 catcccccaa aataatcctc cctatgtgct cgtcgatttt tcgcaagcgc tcccaaaact
      661 cagcaaggat gcaccagaag atttaacgcc agctatcgtt caacgcctca cacaaaaaaa
      721 cttcttcctc accggcaccg gccacaaact cggtgcgggc tttggagcgg ggctcatcgt
      781 tttacccgcg atgcacaccg catggaaaga agcaggactt ttggcgggcg gcaaccaaga
      841 gtatggagtg cgtgcaggta gtcacaacgc ggaagcgatc attgcatttg ccgaagctct
      901 ccatgaaaaa gtgcaaagca attcgtataa gctatggaaa aacatagctc accactttga
      961 gaatcggctt atgcgaatac tcaacagatt ggatgtaaaa attttacctg aaaataagaa
     1021 taacaacgac aatcgtgccc ccggaacaac actgttactg ctccccaata cccctatcga
     1081 cttcctggtg atggcactcg accaagaagg tattaccgtt tcaacaggaa catcgtgtaa
     1141 atcccgctct cgtacacctt cggcagcagt tttggcaatg gggcttagcg aacaaaaagc
     1201 cctcagcatc gtacgtcttt catatagtca agagttgaag gaagccgaca tggaatacac
     1261 ggcgacaacg tttgagaaaa ttttaactgc gatgaaggat tcgtactaaa agtattttac
     1321 taccagtagc tagatagggc aaaattaaac cacttaaagt ggattgaatc tgggcgagag
     1381 aaacaatttt gggtatgtgg tgtatatctt tcaataaatc gtatcgcctg ccacttgagt
     1441 tgttcatcgg taacaaagtt atagccttgt ttttccattg ctaggtgtcc caagaataac
     1501 tccagtgaaa ggcgaataaa cgctgacatc gtaatcctta aatgcattgc agccaaccta
     1561 ataagcatct catcttcgcc atacaaacac accacatgtc gatgtacttt atctgttatt
     1621 tgcacaccac cttttgcttt actatgcgct gtaattagca ctgggttcca acataaattt
     1681 ttctttctcg ccaaacgtaa tacacaataa cgcgtaattg tcgaaaatgt ttttttccga
     1741 gtcactgcta acgagacaat cctttcccat tgaattggcg gcaaatgcac ctccaatata
     1801 ttcataattt tgatcgatcg cttttccaaa aaagacggct gttttctgca tttcataaat
     1861 tccttacata cagagggagt tgtctcttat tagaaatttt aaccctctat atattaaata
     1921 ccaaaaaagg ctgaattttt ctcacttttg gcaaaaaaag taaaattttt ataaaaaaat
     1981 gatattttat aaaaatagct atggaattcc ttagctattc tttaactcca tagctatgga
     2041 atttcatggc aaatactcca caaaacaaaa acatacccat aaaaatataa aaaacggcca
     2101 tccccccata tgcaaataac gtaaaccgtt tcattaatgc tataggggta taaagagtat
     2161 cgactgtatt gagcacagaa tagcgaccga taaaatcgcc acggttatcg acagcatagc
     2221 tcaagccact catcgctgca cgcactaacg gtatgccgtt ttctacagct ctaagcgacg
     2281 ccgctcctgc atgttgccat gccgcatagc catcgccaaa ccaggcatcg ctcgacaaat
     2341 tcacgatgag cgatgcgtgt tgcgtgtggc tgtcttgtgg aaaaagtcct tcatagcaca
     2401 atataggttt aatgaggacg ttgcgcggat gctgtggatt tccatagcgt tcgcgtattt
     2461 tttgtggtga gttaagatac ggtaagattt cctccaattc cttacgcggt gggagcgatg
     2521 ctccattttc tgtgagagta attatcatct cttgcgcagc ggttgccgca tgaaatcggc
     2581 ctgcgtcagg aaacacacgc ctcagtgaag ggagaaattt ttcaagaggt aagtattctc
     2641 cccacggtac aagacggcgt tttaagtagg tggtgtttgt tgcggggcgc gctgcgagct
     2701 ctgaataaag atgaccattt tgcatggtca tgttttggta gagaacggta gcgccggtgc
     2761 catgcgacaa aaaggtgagg acaccatcga acgtggatga atagatggaa gtattttctt
     2821 tttgggaatt ggcagagtgg aatggcatcg atgcttccgg ccaaagaatt aaatccacac
     2881 gtgggtgtaa aagctcgcct tcggtggatt gcgtaaagag acggttgatg gcctcggtgg
     2941 cgaatgctgc atcgccgcgt tttttttcat ccgccgcacc gatattcgtt tgtaccgtca
     3001 gcacatgcaa ctgcggtgcg gcggtatttt gcggtgtgtg gtataacact cctcctacaa
     3061 aaaacagaat caatatcagg gtaaatccca ctttcatttt taaaaacact accaacgctt
     3121 tttctttttt aattagaata aaaaattgca aaaagatgga tgcaataacg aaggcaagaa
     3181 aggagagaaa gtacaccgat cccactgccg cccactggcg caagtattcg ctgccagcaa
     3241 gtgtgtagcc ccacgaccac gcaaagagtt cgggagagaa ggcgtcgatg agtgctgcac
     3301 acgctgccca aaaaaacaaa gcgcggtagg taaaagcatt ggttttgaag aaatagcgtt
     3361 tgagtaaaaa aaagaaaagt ggttttgttt ggaaaagtat tgtatagagg attaccaaga
     3421 acaccgtcag catgtagtgt gcgccggtgt aacggtggac tgtagggaga atccaaccaa
     3481 aggtgataac ggaaagagaa aatgcaaaaa taaaacttgg caccaacaca cggcgcaacg
     3541 atgcaccgtg ttgttcatgg gatgcccata aaaggactcc cgcggcaaca atacctacag
     3601 gaaaataatt aaacggcgcg agtcccaaaa aaatacctgt tgcgtggact acggcaaaac
     3661 aggtttgtct tacataaaac ttttgaaaaa gcgagtacaa agattatttt aaattgtttt
     3721 tctttaagta ttcttcgtat tgcatccgcc gtgcatattc ttcggcttct tctttgccta
     3781 gagttttaat tctcaactcg tcgagctttt tatttttctc tacttcgttt agcgaaggat
     3841 tttcacgaat aagtttctgc gcctctgttt cgtatgtgtt gatgcgcgca cgctcttctt
     3901 ttacttcggt gtctactgcg gccatccgct ttgccgcttc tttaccaaaa tactcttccc
     3961 gcatcgcttg cgttttagtt tctgtggtac ccttgcgttg catttcgtcg ctacgcagca
     4021 tcacctcggt ttcgtaacgc gtgtacggcg attcgcgttc ttcgtaggcg ctattgtaat
     4081 cgcccaagtt tctacgacgc agctcatagt actttttcac ccgctcatcg gcaggcatat
     4141 ctttggaaga g
//
LOCUS       DAOMPD010000226         4141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8945_length_4141_cov_2.276065, whole genome shotgun sequence.
ACCESSION   DAOMPD010000226 DAOMPD010000000
VERSION     DAOMPD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8945_length_4141_cov_2.276065"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PLY93_08585"
     CDS             complement(<1..1225)
                     /locus_tag="PLY93_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRP69574.1"
                     /translation="MPHYQYTAFNKKGQKVTDVVEAISVQAATQKLKSQNLYVREIRA
                     DSTKRDRELFPFLSNLLYRIKRKDTGIFSRQLGTLLGAGIPLNEALSDISEQTENKHL
                     RKIIQEMKQQVTEGKSLSETIKQYADVFPPVYSSMIRVGEATGNYEPTLKRLADLEER
                     NEELKSRAITAMIYPMFMLVISVGVIFFLMTSVVPQLSDLFSGFKAELPLITRVVIGL
                     SKIFSNFWYVMILAIAGGIYAFVRYRSTPAGKLKTDKMIMRIPFFGNLLRRIQVGLFC
                     RNLGILAESQVPLLDALKIVSGTVTNSVFIDEINVAVQQIKEGSSLRESLKDSVILPQ
                     MAKGMISAGESTDRLAELLIKAAEIMECEVDSAVRRMTQSLEPIMIVFLGGIVAAIMA
                     AVMMPIMSGPTNDVTF"
     gene            complement(1227..2957)
                     /gene="gspE"
                     /locus_tag="PLY93_08590"
     CDS             complement(1227..2957)
                     /gene="gspE"
                     /locus_tag="PLY93_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRP69575.1"
                     /translation="MRKFGEILLDDGLIDEAELKKALALQKKSPLKLGQILVKKGLLT
                     EEDALKTLSKQYEMPYVEKLEVVNVDALVEKVPIKFVQKYRVAPYALNEREIKIALSD
                     PSLLHPLDEFRLLFIGYDVIPVLSRESEILRVIHNAYESKVRAEDSMLEMSDGLEILD
                     NIQDLQDSSELANQAPIIKLVNMILSNAVNEKASDVHFEPHENKFSIRFRIDGILHEM
                     MSPPKSVQNGVISRVKIMANLNIAENRLPQDGRIRLRFGGNDIDIRVSSLPTQHGERL
                     VLRLLNRTAAKYDLANIGFEKQTYESFLDMINQPNGILLITGPTGSGKTTTLYAALVH
                     LNSPERNIITVEDPIEYQIEGIGQVQARSKIGFTFAEGLRSILRQDPDVIMVGEIRDE
                     ETARIAIQSSLTGHLVFSTLHTNDAPSAITRLLDMGIEPFLVTSTCRGIMAQRLVRRL
                     CTHCKKPRMIPLKELAELGLSYAEKKLQLKSKSKKAKSPLPTKATKLQIYEPVGCKEC
                     MNTGYRGRMGVYEFMPLTAKIREMVLQRASLDDIKNKAIDEGLITLRNAALRQVVNGA
                     TSLDEALRIT"
     gene            complement(2987..>4141)
                     /locus_tag="PLY93_08595"
     CDS             complement(2987..>4141)
                     /locus_tag="PLY93_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRP69576.1"
                     /translation="EKKDCDEEKDPECGIIKQDGNVHVYRLENMQAENIEATLKKIQL
                     PVEPLRPLASTAAAGTTTPPPAATQANVQKKPIDVVAHKETNSIIFVGTNEEFDLVKS
                     LIKRIDLQRQQVLLEVLIVEVGVDNTNEFGLDWIMGKGYSAQFNSTKIATSSGYIGTD
                     GQIDTSRISTLPGLTLAFAEQSISGLIGVLNAHIGRSNYMVVSAPQILTLDNQEAEIN
                     VGEDRPVRVNSLSSAISAAGLTQSQSFEYRPVGVKLKFTPQVNKNRMVTLNLYQEVKS
                     ITSTDSSSGNPIIGKKDIKTYVRVQDGQTIVIGGLVSSDRRTEMKKVPILGDLPLLGY
                     LFKRTGTTNKRNNLLVFITPHVLTNRALADKVTEDLRKDQIKELKRSQTE"
BASE COUNT          978 a          959 c         1043 g         1161 t
ORIGIN      
        1 gaaaagtgac gtcgttcgtc ggcccgctca tgatcggcat catcaccgcc gccataatcg
       61 ccgcgacaat gccgcccaaa aaaacaatca taatcggctc taaagactgt gtcatgcggc
      121 gcacagcgct gtcgacttcg cactccataa tttcagcggc cttgatgaga agttcggcca
      181 aacggtcggt actttcaccg gcggaaatca tcccctttgc catttgcggc agaattaccg
      241 aatctttgag cgattcgcgc agagacgaac cttctttaat ttgttgcaca gcaacgttga
      301 tttcatcgat gaagacagaa ttggtgacag taccagaaac aattttgagc gcatctaaaa
      361 gcggcacttg gctttcggcg agaataccca agttgcggca gaaaagcccc acttgaatac
      421 ggcgcaaaag attgccaaag aagggaatcc gcataatcat cttatcggtc ttgagtttac
      481 ccgccggcgt cgaacggtaa cgcacaaacg cgtagatacc acccgcaatc gctaaaatca
      541 tcacatacca aaaattggag aagatttttg aaagaccaat caccactcgg gtgatgagtg
      601 gtagctctgc cttaaagcca ctgaaaagat ctgaaagttg tggcacgacc gatgtcatca
      661 aaaagaaaat cacccccact gaaataacga gcatgaacat cgggtaaatc atcgctgtga
      721 ttgcacggct tttgagttct tcgttgcgct cttccaaatc ggcaaggcgt ttcagcgtgg
      781 gttcgtagtt accggtcgct tcccccacgc gaatcatgct cgagtagacc ggcgggaata
      841 catcggcgta ttgtttgatc gtttcggaga gtgattttcc ttcggtcact tgctgcttca
      901 tctcttggat aattttgcgt aagtgtttgt tttcggtttg ctcgctgatg tccgacaacg
      961 cttcgtttag gggaatgccc gcccctaaaa gagtgccaag ctgacgcgaa aaaatccccg
     1021 tgtctttacg cttaatgcgg taaaggagat tgcttaaaaa aggaaaaagt tcgcgatcgc
     1081 gcttggtgga gtcggcgcgt atttcacgca cgtataagtt ttgcgatttg agtttctgcg
     1141 tcgccgcttg cactgaaatg gcttcgacaa cgtcggtgac tttttgtcct tttttattga
     1201 acgcggtgta ttggtagtgc ggcattttag gtaattctga gcgcttcgtc gagcgatgtc
     1261 gctccgttta ccacttgccg cagcgcagcg ttacgcaacg taatgagtcc ctcgtcgata
     1321 gctttatttt taatatcgtc gagagaggca cgctgtaaaa ccatttcacg aattttggcg
     1381 gtcagcggca tgaactcata aactcccata cggccacggt agccggtgtt catacattct
     1441 ttacaaccga caggttcata aatttggagc ttcgttgctt ttgtcggtaa gggggattta
     1501 gcctttttcg attttgattt cagttgtaat tttttttcag catagctcaa acccaattcc
     1561 gccaactctt taagagggat catgcgcggt tttttacagt gtgtgcataa acgccgtacc
     1621 aaacgctgcg ccataatccc ccgacaggtg gatgtgacga gaaaaggttc aatccccatg
     1681 tcgagaagac gggtgatcgc cgatggagcg tcgttggtgt gcagcgtaga aaaaaccaag
     1741 tggccggtga gcgacgattg aatcgcaatg cgcgcggttt cctcgtcgcg gatctcgccc
     1801 accataatca cgtcggggtc ttggcgcaaa atagagcgca aaccctcggc aaaagtaaaa
     1861 ccaattttgg agcgcgcctg cacttggcca atcccttcga tttgatattc gatgggatct
     1921 tcgacggtga tgatgttacg ctcagggctg ttgaggtgta caagcgcagc gtagagtgtc
     1981 gtcgttttac cggagccggt tgggccggtg atgagtagaa taccgttggg ttggttgatc
     2041 atatcgagaa agctctcgta cgtttgtttt tcaaaaccaa tgttggctaa gtcgtatttc
     2101 gcagcggtgc ggttcaaaag gcgcagtacc aaccgttcgc cgtgttgggt ggggagcgac
     2161 gaaacccgaa tatcgatgtc gttgccgcca aagcgtaacc gaatgcgacc gtcttgcggt
     2221 agacgatttt cggcgatgtt gaggttcgcc ataattttaa cgcgcgaaat gacgccgttt
     2281 tgcaccgact tgggcggaga catcatttcg tgtaaaatac cgtctatgcg aaagcggatc
     2341 gagaatttat tttcatgcgg ctcaaagtga acgtcggacg ctttttcatt taccgcattc
     2401 gataaaatca tattcacgag tttgataatc ggcgcttggt ttgcaagctc ggaggagtct
     2461 tgtaaatctt gaatattatc caaaatttca agaccgtcgg acatctcgag cattgaatct
     2521 tccgcgcgca ctttggattc gtaggcgttg tggataacgc gcagtatttc gctttcgcgc
     2581 gaaagaaccg gaataacgtc gtaaccgatg aaaagaagac gaaactcgtc gagtggatgc
     2641 aggagcgagg gatcggagag agcgatttta atttcacgct cgttgagcgc gtacggagcg
     2701 acgcggtatt tttgaacaaa tttgatgggc actttctcaa cgagtgcatc gacattcacc
     2761 acttcgagtt tttcgacgta gggcatttcg tattgttttg aaagtgtttt gagcgcgtct
     2821 tcctcggtga gcaatccttt tttaacgagg atttgaccga gtttgagcgg agattttttt
     2881 tgtagcgcga gcgccttttt gagttctgct tcatcgatga gaccgtcgtc gagaagaatt
     2941 tcaccaaatt tacgcatgcg ttactttgat actcgtggcc gcattgttac tcggtttggc
     3001 tgcgcttgag ttctttgatt tgatcttttc ttaaatcttc ggtaactttg tcggcgagcg
     3061 ctcggttggt gaggacgtgc ggagtaataa aaacgagcaa gttattacgc ttgttcgtgg
     3121 tgccggtgcg tttgaataaa taccccaaga gcggcaaatc tcccaaaatg ggcacttttt
     3181 tcatttctgt tctacggtcg ctgctgacaa gaccaccgat gacaatcgtt tgtccgtctt
     3241 gaacgcgtac ataggtttta atgtcttttt taccgatgat ggggtttccc gaactcgagt
     3301 cggtgctggt gatgcttttc acttcttggt aaaggttgag agtcaccatg cggtttttat
     3361 tgacttgcgg tgtaaattta agtttaaccc caacggggcg atattcaaag ctctgcgatt
     3421 gcgtcagtcc cgccgcgcta atcgccgacg aaagactatt gacgcgcacg ggtctatctt
     3481 cacccacatt gatctctgct tcttggttat ccaatgtcag aatttgtggc gccgaaacta
     3541 ccatataatt tgagcgacca atgtgcgcgt tcaaaacgcc gataagacca gaaatcgatt
     3601 gttcggcaaa cgccagcgtc aaccccggaa gggtgctaat acgggaggtg tcaatttgac
     3661 catcagtgcc aatatatccc gagctcgtcg caatttttgt actgttaaat tgagcgctgt
     3721 accctttgcc cataatccag tcgagaccga attcgttggt gttgtctaca cccacttcaa
     3781 caattaacac ttcgagtaac acttgttggc gttgtaaatc gatgcgcttg atgaggctct
     3841 taactaaatc gaattcttcg ttggtaccga cgaaaatgat actgttagtt tctttatgcg
     3901 ccaccacatc gatcggcttt ttttgcacgt tggcttgtgt ggctgcaggg ggcggcgttg
     3961 ttgttcctgc cgctgcggta gaggcaagtg ggcgcagcgg ttcaaccggt agttgaattt
     4021 ttttgagagt cgcctcgatg ttttcagcct gcatgttctc aagacggtac acatgcacat
     4081 tcccgtcttg tttaatgata ccgcactcgg ggtctttttc ttcgtcgcag tcctttttct
     4141 c
//
LOCUS       DAOMPD010000227         4134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8960_length_4134_cov_2.475116, whole genome shotgun sequence.
ACCESSION   DAOMPD010000227 DAOMPD010000000
VERSION     DAOMPD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8960_length_4134_cov_2.475116"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..359)
                     /locus_tag="PLY93_08600"
     CDS             complement(42..359)
                     /locus_tag="PLY93_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69577.1"
                     /translation="MYNLYRGGRYVLGTGNYLRRMFMESDDEFSARLKWASYVNYPKK
                     IVNTYKSYIFYEKVIVAPPEPLDLEHIANRLCLHALIGGEAYLFEAQGRIEVFSRREV
                     ANE"
     repeat_region   370..520
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=370..398
                     /rpt_unit_seq="ccactattccccacgcatgtggggatgaa"
     gene            complement(677..2359)
                     /locus_tag="PLY93_08605"
     CDS             complement(677..2359)
                     /locus_tag="PLY93_08605"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRP69578.1"
                     /translation="MKKGFQKLLEQKLHLFILVLFSAIAASNCGKTYPLSMATSTSLT
                     GAPVFDLLNGKKWQAEPGAEYVKVHLYFDEPLMLKEAEINACDKFDDNVHMFINFDEF
                     NYNLKTKKGDSKLSVTLDTPVNARSVTFNLNRNSNPCLSSVSFWDKSGKKVKFSTPEI
                     VAGEVKPSKTSKSKAYHPLNLFDSRYETAWASSPGEKDIFLDFNFANETKITALKIWN
                     GYQRSPLHCIRNGRVKTIEVTGDGNYNERISVADEMDSQIVKLPKPFKGKNLRLRMAE
                     LYRGELEGVVISELRFLNGNHSILFNPAPLAKDVSTENRELFRTAGFEDILNKGLGSY
                     EDTSTDSIDTRIVTAKGGLRIRESASTTAPVVILVPQGSKVEYAGEMGEELKIGNDTG
                     HWIKVSFAGKTGWAFDAFLESRNESAASSSIWKLRLRSDGSLYIQGTTEKSLLDYEAA
                     KKDTTNTSFYAVGNFEAEKPVPNEIDLNLFGFVRVVEVRSSEYVEMGHDCNGCGRDCA
                     QVKNNKGEKVFIQKARLIKSGNKYFLLNKSPNPQIPFTTVELEIEKPLPKES"
     gene            complement(2428..2991)
                     /locus_tag="PLY93_08610"
     CDS             complement(2428..2991)
                     /locus_tag="PLY93_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69579.1"
                     /translation="MGPLRRLFLSAIFLFSAACHPENSVPLQSENGDTRVKIEWVYKN
                     FPAEITIHELKRGKEAVLWQTGSALDIKDTPIGDEIDQGVFAVQQGKEKYFVLAAKNK
                     TAKPIYFFAAPHHLLPAEAGLGFQFKCLCVNHLFAIKPNETWYRIVRLRLTRENHIAS
                     ITISHDIVGIDKKGREEYLIDAAEPKG"
     gene            complement(3043..4026)
                     /locus_tag="PLY93_08615"
                     /pseudo
     CDS             complement(3043..4026)
                     /locus_tag="PLY93_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803639.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
BASE COUNT         1017 a          930 c          970 g         1217 t
ORIGIN      
        1 aaccatttat ctgtactcgt ctctattgtg tagatggcaa cctattcgtt tgcgacttcg
       61 cgccgtgaga aaacttcgat tcgcccttgt gcttcaaata aatacgcctc gccgccaatg
      121 agtgcgtgca aacacaagcg attcgcgatg tgttcgaggt ctaacggctc gggcggggca
      181 acgattactt tttcgtaaaa aatatagctt ttgtaggtgt ttacgatttt tttagggtag
      241 ttgacgtacg acgcccattt gagccttgcg ctaaattcat catccgactc catgaacatc
      301 cgacgcaggt agttgccagt accgagcacg taacgcccgc cacggtagag attgtacaag
      361 tcttcaatac cactattccc cacgcatgtg gggatgaagg ctattagttg taaaactaac
      421 ctcatattgt ccactattcc ccacgcatgt ggggatgaat tacgcggact ctttgttcag
      481 cgacaataca tccactattc cccacgcatg tggggatgaa ccgaccgcgc atgagcggcg
      541 caaagtcgcg cacgcagaaa ataccatggc gctaaataag tgaaatgtaa acaaaagttt
      601 tatatagcct ttgcatactg aaatcctcct tacaatttat ttccgaattt aaaacgtatc
      661 tcccgcatct ccccgctcaa ctttctttgg gaagtggttt ttctatttct aactctactg
      721 tggtaaacgg tatttgtggg ttgggcgact tattgagaag aaagtatttg tttccgcttt
      781 taataagcct tgctttctgg ataaaaactt tttcgccttt gttgtttttt acctgagcgc
      841 agtcgcggcc acaaccatta cagtcgtgcc ccatttcaac gtattcggag gaacgcactt
      901 cgacaacccg cacaaaacca aaaaggttta ggtcaatttc attcggcaca ggtttttctg
      961 cctcaaaatt accgacagcg taaaaagaag tgttggtggt atctttcttt gcagcctcgt
     1021 aatcgagtag tgatttttca gtagtgcctt gtatgtataa actaccatca ctacgtaacc
     1081 gtaatttcca aatactgctt gaggctgcag actcattgcg actttcaaga aaagcgtcga
     1141 aagcccaacc cgttttacct gcaaaagata cttttatcca atggccggta tcgtttccaa
     1201 ttttgagttc ttcgcccatt tcacctgcgt attctacctt acttccctgc gggactaaaa
     1261 tcacaacagg tgcagttgtt gatgcactct cacgaatacg caatccccct tttgcagtga
     1321 caatccgtgt atcaattgaa tcggtagatg tatcttcata agaaccaagg cctttgttga
     1381 gaatgtcttc aaaacctgct gtacggaaaa gttcgcggtt ttcagtagag acgtcttttg
     1441 caaggggtgc tgggttaaag aggattgagt ggttgccatt caagaaacgt aactcgctaa
     1501 tgacaacacc ttccagttct ccacggtata gctcagccat gcgtaagcgt aaattttttc
     1561 ccttaaaggg tttaggtagc tttacgattt gtgaatccat ttcatcggcg acagaaatac
     1621 gttcgttgta gttgccatcc cccgtaactt cgatggtttt gacgcggcca ttacgaatgc
     1681 agtgcaatgg tgacctttgg tagccattcc aaatttttag cgcagtaatt tttgtttcat
     1741 tcgcaaaatt aaaatcgaga aaaatatctt tttcacccgg tgaagatgcc cacgctgttt
     1801 cgtagcgcga atcgaaaaga ttgagagggt ggtacgcttt cgacttacta gtttttgaag
     1861 gttttacttc gcctgcaaca atttctggcg tgctaaattt tactttttta cccgatttat
     1921 cccaaaacga cacgctgctg aggcatgggt tgctgtttcg atttaggttg aatgtcaccg
     1981 aacgcgcatt gacgggagtg tcgagagtga cagatagttt gctgtcgcct tttttcgttt
     2041 tcaaattata attgaattca tcaaaattga taaacatgtg aacgttgtca tcgaacttat
     2101 cacaggcatt aatttctgct tctttgagca ttagaggctc gtcaaaataa aggtggactt
     2161 ttacgtattc agcgcccggt tcggcttgcc attttttgcc attgagtaaa tcgaacaccg
     2221 gcgcacccgt aagcgaagtg gatgtcgcca tcgaaagggg gtaggttttg ccacaatttg
     2281 atgcagcaat agcagaaaat aatacaagta taaaaaggtg taatttttgt tccaaaagct
     2341 tttgaaatcc ttttttcata aatacttcct taaatgaatt ttttaatcaa cacgagttcg
     2401 atactgaacg ttagaaatta ggcgtgctta acctttaggc tctgcggcgt caattaaata
     2461 ttcttcacgc ccttttttat cgatacccac aatatcgtgg ctaatggtta tcgaagcgat
     2521 gtggttttcg cgtgtgaggc gcaggcgtac aatgcggtac catgtttcgt ttggctttat
     2581 agcaaatagg tggttgacac aaaggcattt gaactggaaa cccagccccg cttctgccgg
     2641 taaaaggtgg tggggtgcag caaaaaaata aatcggcttt gctgttttat tttttgcggc
     2701 cagaacaaaa tacttttctt tgccttgttg tacggcaaaa acaccctgat caatttcatc
     2761 gccaatgggg gtgtctttaa tatccaacgc agaaccggtt tgccacagca cagcttcttt
     2821 gccgcgcttg agttcatgga tagttatctc tgccggaaaa tttttataga cccattcaat
     2881 ttttacccgc gtgtcgccat tttcactttg tagtggaaca gaattctctg ggtggcacgc
     2941 tgcggagaat aagaaaatgg cagataaaaa aagccggcgg agaggaccca cgccggcaat
     3001 ctatgcgcaa tcttttgcgc gtaaagtgtg ttttagcttt tattaactaa accaacgctt
     3061 cgttgcttcg gcgccgatgt cgatgccggt tgcgcgggcg cgttgtacaa tcgtccagaa
     3121 accacggtag gtggcgcggt cgtgcagttc tccttcgtgc tggatggggc cccacgctgc
     3181 atcctgtgct gcgaggagca ccgcgacgcc tttttgcact tcagagagat cgggtttcat
     3241 cgccgcgaca atcgcgtcga tttgggtggg gtaaatcgac cacatgcgca aaaagccaaa
     3301 ctcgttgcgt gcgcgcgatg cgtctgaaaa agtttggtcg taatttttga ggtcaagcgt
     3361 cacgttgtgc gcgggtacca cgccgttcgc gagcgcagcc gccaccacgt tgcctttagc
     3421 gcgcgcgagg agcttgtggt cgaactgacc gggacttttc atgttagaaa gcgcaatcgc
     3481 gccattgtga cctgaaataa aatccataag gccaaagtcg agcacttgca accaaggtaa
     3541 cgcagagatt ttttctacgt cgcgcaatgc tccgtgtgtt tccacgagga cgtggatggg
     3601 aatttcgcgt ttgatgtttg ctttttttgc ggcggcttgg atgtactcga tcatttcttt
     3661 gacttgttcc taagcggtgg gtttagggat ggtgatgtag gcaatgcgtt cgccacaacc
     3721 gggaaccaga atatccacgt cttgcttcca gtacttactg gtataatcgt gtatgcgcac
     3781 gcccgccata ttgtgcttgt tgtgctcact gttggttaag cgtacaatca tttcggcatg
     3841 ttctttttct tggccggtgg gtgcgccgtc ttcgcagtct tgggtgatgt cgaatacggg
     3901 gcctttttct tgttgaaagc tatacgcttt cataatcatt ttttcgttac cggcgaagtg
     3961 ctcgcatgcg gcaatcacgg ggaaaggctt ttcgccagag aatagtgctt ctttagggtg
     4021 tatcatggtg tggaaaaatt ggttttgcga gacagggcgt caatgaagag gagaaggagc
     4081 ctctgtctaa cccacggaca tacctgacaa atgctacgcc gcaattgggg ggta
//
LOCUS       DAOMPD010000228         4131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8967_length_4131_cov_2.416094, whole genome shotgun sequence.
ACCESSION   DAOMPD010000228 DAOMPD010000000
VERSION     DAOMPD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8967_length_4131_cov_2.416094"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLY93_08620"
     CDS             complement(<1..186)
                     /locus_tag="PLY93_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69580.1"
                     /translation="MKKFWENLSARERTYLVILAAFAVVIIIFLTGRSFATYMSNLAE
                     RSQNAESNTLRLESLGRE"
     gene            complement(190..>1527)
                     /locus_tag="PLY93_08625"
     CDS             complement(190..>1527)
                     /locus_tag="PLY93_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69581.1"
                     /translation="DVVFDSHLTTAVSGKATRAIVAGSRKDILIPYLSEFHKKSIPVL
                     GIYTPHDALYRLHPFTGLTSCIMLHISRLVSIIVIVVEGEWRFARVLPVGYDWLIAKL
                     KSKWKNDAATIHRLLQALPTSLDDSSEVDTFKKAHKITNAKFAMLTDAVADFTHVMRR
                     ELAASLRNLQLDADDEKIAQEKLTGENIDAKPFSPESMTVALSSDLNNAVVLENALAS
                     NLKYQVEKFPYERTPLALLNREHLITMGGAYSQTTTSKLNLLKDDLKKWVVSSRAGAS
                     RLILALGVAAVLLFSLSFLIDLKRRGYELREWETRQAKVFQQFFPGVPLPDSESYLST
                     AMQLVKKEKQKTEILDNFFNSPRLSAVLVALHQNISTDAQLEITSFYFNDRGVEINGL
                     VPDYARLEDMKRSLAKSTLLTAVESKNENSSPGPNGQNRIKFTLFCKVKKVEQ"
     assembly_gap    1528..1537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1538..1839)
                     /locus_tag="PLY93_08630"
     CDS             complement(<1538..1839)
                     /locus_tag="PLY93_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69582.1"
                     /translation="MARTPVVIEINNYEIKGLAFRNGLAGRTFFDAESILLNPERGIA
                     EQATAFVAEKYSNAIGVYFNVPYESLFTRELSFPFADRRKVREVLPFELEPQIPND"
     gene            complement(1865..2938)
                     /locus_tag="PLY93_08635"
     CDS             complement(1865..2938)
                     /locus_tag="PLY93_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRP69583.1"
                     /translation="MKSVAKKTPRFFRIKKRGAIILITTTLILTLTTSVALVYVRFAL
                     NEYRGVSTAGNREKSLQLAQSGLETGILLLTRVPVSSLYDFGIFTLAPTIPLGGGMIT
                     LSLSEESGKLNVNRLVRMFEDKTDERQLEAFQRLSIALGIPADLWDAVVDYIDENNTP
                     MPRGAERNDYERLVPPRRIKNGRMQSIEELLFIPGFDARLLYDDSRSQDRIKNTSTAS
                     LSDAEKAAISPADYVLANNISTYLVYNAANAGDLININSAPYHVWLSLSAFMTPAAAK
                     RIVTERVKRGGRFKSVAELATLPELQIPTTGSITLFQEIASRVTDQNQLYKIVADASI
                     DSQVAQVMGVLDPNAKRLVFYAE"
     gene            complement(2940..3815)
                     /locus_tag="PLY93_08640"
     CDS             complement(2940..3815)
                     /locus_tag="PLY93_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRP69584.1"
                     /translation="MPLKFFSQNVLVKTVAFAATIFFLLPLHADKKYLSSYVHNQYID
                     SLTCKGTTKLSRKKIPLKKVQYGKSQKGKPLTAYIVPPEKNIDIDDIETVYIIASQHG
                     DERNAARAVGYFVRELRRLSRSYLNHRREVVIPLYNPDGFARNTRFAMGKIDLNRDFP
                     SSNGAKGAPHAAETKAFMHLIKKYPPDFIYNIHQPFRVVLYYPEDEELAQPFAILSDY
                     QSGTDVGYPTPGSLGTWARENKIPTITVELSRSMKKSMAPFIYEEVRLALFHSALGCI
                     PKQAIPSRLEDYLTE"
     gene            3792..>4131
                     /locus_tag="PLY93_08645"
     CDS             3792..>4131
                     /locus_tag="PLY93_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69585.1"
                     /translation="MGKKLQRHRYDVLTSAASRQAFAQAPIPIAAFNRILKKSKMPSP
                     TVQLSEIRAEIDRVCNDIGVDVKKISVSFERSDFNLRTEVNEVTDALLIDAIAVAKRH
                     IENWRIHTFEK"
BASE COUNT         1059 a          929 c         1049 g         1084 t
ORIGIN      
        1 ttcacggcct aaactttcca agcgcaacgt atttgattcg gcgttttgcg aacgctcggc
       61 gagattactc atgtaggtcg caaaagagcg gcctgttaaa aaaatgataa tgacgaccgc
      121 gaaagccgcc aaaatgacga ggtaagtgcg ctcgcgtgcg ctgagatttt cccaaaattt
      181 tttcatccgc tattgttcta cctttttcac tttgcaaaag agagtgaatt taatgcggtt
      241 ttgaccgttg ggtccggggg atgagttttc gtttttggat tcaaccgctg tgaggagcgt
      301 cgattttgcc agggagcgct tcatgtcttc gagccgtgcg tagtcgggta caagaccgtt
      361 aatctcaacc cctctgtcgt taaaataaaa cgaggtgatt tccagttgtg catcggtcga
      421 gatattttga tgcaacgcga ccaaaaccgc cgaaaggcgg ggcgaattga aaaagttatc
      481 taaaatttca gttttttgtt tttctttttt aacaagctgc atcgccgtcg aaagatacga
      541 ctcgctatcg ggtaaaggca ctcctggaaa aaattgttga aacactttag cttgccgcgt
      601 ttcccattcg cgtaattcgt atccacgacg tttaaggtcg ataaggaaag ataaagaaaa
      661 aagaaggact gccgcaaccc caagcgcaag aatgaggcgc gatgcgccgg cgcgcgaact
      721 caccacccac tttttgagat cgtctttaag gagatttagt ttgctcgtcg tggtttgaga
      781 atatgcccct cccattgtga tgaggtgttc gcgatttaat aacgctaagg gggtgcgctc
      841 gtaggggaat ttttcaactt gatattttaa gttcgaggcg agagcgtttt ccagcacaac
      901 ggcgttattt aagtcggagc tcaatgccac cgtcatcgat tcgggactaa aaggctttgc
      961 gtcgatattc tcccctgtta gtttttcctg cgctattttt tcgtcgtcgg cgtcgagttg
     1021 caaattgcgg agagacgccg ccagctcgcg ccgcataacg tgggtaaaat cagccacagc
     1081 gtcggtaagc atagcaaatt ttgcattcgt gatcttatgc gcttttttga acgtatccac
     1141 ttcgctcgaa tcgtcgaggc ttgtgggcag cgcttgcaaa agacggtgga ttgtcgccgc
     1201 gtcatttttc cacttgctct ttaatttggc gatgagccag tcgtacccca ccggcaaaac
     1261 gcgggcaaag cgccattcgc cttcgacgac aatgacgata atcgacacga ggcgtgaaat
     1321 gtgcagcatg atgcacgagg taaggccggt aaaaggatgc aggcgataga gggcgtcgtg
     1381 cggcgtgtag atgcccaaca ccgggattga tttcttgtgg aattcactga ggtaaggtat
     1441 tagaatatct ttgcgtgaac ccgcaacaat cgctcgagtc gctttccccg aaacggcggt
     1501 agtcaggtgg ctgtcgaaaa ccacgtcnnn nnnnnnnaaa tcgttgggta tctgtggttc
     1561 gagttcaaac ggcaacactt cgcgcacttt gcggcggtcg gcaaaaggaa aactgagctc
     1621 gcgcgtgaaa agactttcat aaggtacgtt aaaataaaca ccgattgcat tggagtattt
     1681 ttcagcgaca aaagcggtgg cttgctcggc aataccgcgc tcgggattta agagaatcga
     1741 ttcggcatca aaaaacgtgc ggccggccaa gccattacga aacgcgagac ctttgatctc
     1801 gtagttatta atttcgatga cgacgggtgt gcgcgccata atcaattatc gtaaataaaa
     1861 agcctcactc ggcgtaaaaa acaagtcgtt ttgcattcgg atctaaaaca cccataacct
     1921 gcgctacttg tgaatcgatg gacgcgtcgg caacaatctt atagagttga ttctggtcgg
     1981 taacacgcga ggcgatttct tgaaaaaggg tgatgctccc cgttgtcgga atttgcaact
     2041 caggcaatgt ggccaattcg gcgacgctct taaagcgtcc cccccgttta acgcgttcgg
     2101 tgacaatgcg tttggcggcg gcgggtgtca taaacgccga aagagatagc catacgtggt
     2161 acggtgcaga attgatattg attaagtcgc cagcattggc ggcgttgtag acaagatagg
     2221 tgctaatatt attggcgagg acataatcgg cagggctaat cgccgctttc tcggcatcgg
     2281 aaagcgaagc ggttgaagta ttcttgatgc ggtcttgcga acgtgaatcg tcataaagaa
     2341 ggcgagcgtc aaatccaggg atgaatagca attcttcgat ggattgcatc cgtccgtttt
     2401 tgatgcggcg tggcggcaca aggcgttcat agtcgttacg ctctgcgccg cgaggcatcg
     2461 gtgtgttgtt ctcgtcgata tagtcgacaa ccgcatccca caagtcggcg ggtataccca
     2521 acgctatcga taaacgctga aaagcttcca gttgacgttc gtctgttttg tcctcaaaca
     2581 tacgcaccag tcggttcacg ttgagcttac cgctttcttc gctgagcgaa agtgtaatca
     2641 tgccgcctcc caaagggatc gtgggtgcga gagtaaagat accaaaatca taaagcgaac
     2701 ttaccggtac tcgtgtcaaa agaagaatac cggtttcaag cccactttgc gccagttgta
     2761 acgatttttc acggttgccc gctgtcgaga cgccacggta ttcgttcaaa gcaaaacgca
     2821 catagactaa cgcaaccgag gtagtgagcg ttagaatcaa cgtcgtcgtg attaaaataa
     2881 tggctccgcg ttttttgata cgaaagaaac ggggtgtttt tttagctact gatttcattt
     2941 tattcggtta gataatcctc aaggcgcgaa gggatagcct gtttcggtat acatcctaac
     3001 gctgagtgga aaagggccaa gcgcacttct tcgtaaataa aaggagccat cgattttttc
     3061 atcgaacgcg aaagttcaac tgtgattgtg ggtattttat tttcgcgcgc ccacgtcccc
     3121 aacgatccgg gagttggata acctacatct gtcccacttt ggtaatcgga taaaattgca
     3181 aagggttgcg caagctcttc gtcttcggga taataaagca caacacgaaa aggttgatga
     3241 atgttgtaaa taaaatcagg tggatatttt tttattaaat gcatgaacgc tttcgtctcc
     3301 gcagcgtgcg gcgcgccctt cgcgccgttt gagctgggaa aatcacggtt taaatcaatt
     3361 ttacccattg caaaacgagt gttgcgtgca aaaccgtcgg gattgtaaag ggggattacc
     3421 acctcgcgcc gatggtttaa gtagctacgc gagagtcgac gtagctcgcg cacaaaatac
     3481 cccactgcac gcgctgcgtt ccgttcgtcg ccgtgctgcg atgcaatgat ataaacagtt
     3541 tctatatcgt cgatatcgat atttttttca gggggtacta tgtacgcggt gagcggttta
     3601 cctttttgcg attttccata ttgaactttt ttgagtggga tttttttgcg agagagttta
     3661 gttgtccctt tacaggtgag cgaatcgata tattggttat gtacatacga cgaaaggtat
     3721 tttttgtcgg cgtgtagtgg caggagaaaa aaaatagtag cggcaaatgc aactgttttc
     3781 acaagtacat tttgggaaaa aaacttcaac ggcatcgcta tgatgttttg acatcggcgg
     3841 catcgcgtca agcgttcgca caggctccta tcccgattgc cgcgttcaat cgtattctaa
     3901 aaaagtccaa aatgccaagc cccactgtgc aactcagcga gatacgagcc gagatcgacc
     3961 gtgtttgtaa cgacatcggt gttgatgtaa aaaaaattag cgtcagcttt gagcgctccg
     4021 attttaattt gcgcaccgag gtcaacgaag tgaccgacgc actcctcatc gacgccatcg
     4081 ccgtggctaa acgacacatc gagaattggc gtatccacac ttttgaaaaa t
//
LOCUS       DAOMPD010000229         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8985_length_4125_cov_2.873219, whole genome shotgun sequence.
ACCESSION   DAOMPD010000229 DAOMPD010000000
VERSION     DAOMPD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8985_length_4125_cov_2.873219"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="PLY93_08650"
     CDS             complement(<1..1572)
                     /locus_tag="PLY93_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRP69586.1"
                     /translation="MRNNGPVTGREFIQRDDAQLVTKTNLKGVITYANRDFAEAAKMP
                     VDDLIGQPHNVIRHPEIPPAVFADLWSTIRAGKPWAQVLKNRCKDGDHYWVLANVSPI
                     VEGGQTTGYLSARYPVSDEQKRNADVLLKRVAAGGVHLIEGRVVRFPWLDKINFVRRA
                     SIMQKIILSTLLFLLPLVGYIGFWVNRVLTNERMQLHLLGDSAAIGVTAVTGVFFCIG
                     VFTFLRTIFSLKRRTHLVTQDLQRIQDGTYSGYIEVNTEDEIDRLALAMRNMKNRLGF
                     EMVETKLLLQEVLRNKVALDNVSSNIMIADDEGKIVYMNKALTEMLTACESEIRSVLP
                     NFKVAEIVGSSMNKFHKNPELQQGILKSLKTAHTSQIQIGSKTFDLHLTPVIDEKGVR
                     LGGALQWVDRTDELKAQKEIATIVDNATKGDYSARLSLDGKTGFIRDLSVSLNRVSEI
                     TEAALGDIGTIIRAMSKGDITKKIESEYTGKYAELKDQINGMIMTLAEIISNVSSNAE
                     NFTHASEQLSQMAQSM"
     gene            complement(1562..3598)
                     /locus_tag="PLY93_08655"
     CDS             complement(1562..3598)
                     /locus_tag="PLY93_08655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRP69587.1"
                     /translation="MDSLRATFREEALELLSSLEEALLGLEKNLTDDEALSTAFRAVH
                     TIKGSGGIFDFDELVCFSHEVEDLLDALRNKRLPVTLEIAALLITASDHLVALINHFT
                     TDTPESALKHLVSVSNTILLQLRRLMDTSEKAVDIDTSALPQNTKEAEVFNESTHPRF
                     IVRLKTDLYRHGLAPEKALRFLFKYSSLEYIKLITTDLPSAQNFDPEDAILVFEVAFQ
                     KTVSHEDAARELNEAFEFLLSRVSITQIAKAIHTVPEKGAGSGVNPTNENKNPSNANT
                     TKASSVESEQTKASKQLRVSSDKLDLLVDLVGELVMKNGTLQQLSRETQTKEFMRPVS
                     EMTRLVENVRELAMNLRMVPIGRTFHRMERVVRDAAGVSGKMVRLELKGEDTELDKSV
                     IERIGDPLMHIVRNSVDHGIESVEARLEQGKPQEGVVSLSAYNAAGAIVIEVTDDGRG
                     LDLSRILEKARAKGIADTDREYSESEIQQFIFAHGFSTAEAVTEISGRGVGMDVVKKN
                     IDALRGVIRIQSVFGQGMTLRIVLPLTLAIIDGFVVRVGAQKFVFPLESVKECLDFST
                     DALNDDTRNQIFNLRGEALPYTSLSSIFNIEHDSSERASLVIVEDGERRFGIVVDEPL
                     GEHKTVIKPLTGLLANLRGIGGATILGAREMAFIIDVPGIIDLIGKGRRSKYEK"
     gene            complement(3601..3975)
                     /locus_tag="PLY93_08660"
     CDS             complement(3601..3975)
                     /locus_tag="PLY93_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP69588.1"
                     /translation="MPQGTFKIIQIAAQEGRGVVALKGNFTIRYAKEAFFTLLEFFNK
                     FDALALDLSGIESIDSAGFQVLLVLKKESEAQQKKVKLIRHSAPVVDMIELYGAMGLF
                     RDKIVVAQNNRFRYGREIQHFT"
BASE COUNT         1005 a         1071 c          945 g         1104 t
ORIGIN      
        1 catcgattgc gccatttgcg aaagctgttc ggaggcgtgc gtaaaatttt cggcgttcga
       61 ggagacgttc gagataattt cggcgagcgt cataatcatg ccgttgattt gatctttaag
      121 ctcggcgtat ttaccggtgt attcactctc gattttcttg gtgatgtccc ctttactcat
      181 cgcgcggatg attgtgccga tgtcgccgag cgccgcctcg gtgatttcac tgacgcggtt
      241 gaggctcacg ctcaaatcac ggataaaacc ggttttaccg tcgagcgaca accgtgcaga
      301 ataatcgccc ttggtggcgt tatcgacaat cgttgcaatt tctttttgcg ctttgagttc
      361 gtcggtgcgg tcgacccatt gcaatgcgcc acccaaacgc acgcctttct cgtcgatcac
      421 cggcgttagg tgtaaatcaa acgtttttga gccaatttgg atttgtgacg tgtgcgccgt
      481 ttttaacgat tttagtattc cttgttgcaa ctctggattt ttatgaaatt tgttcatgct
      541 gctgccgaca atttctgcaa ccttaaaatt aggcaacacg ctgcggattt cactctcgca
      601 cgctgtcaac atttcggtga gcgctttatt catgtagaca attttacctt catcgtcggc
      661 gatcataatg ttcgacgaaa cattgtcgag cgcgactttg ttgcgtaaca cttcttggag
      721 caacaatttg gtttcgacca tttcaaaacc caatcggttt ttcatgttac gcatcgcgag
      781 cgctaaacga tcgatttcgt cttcggtgtt gacttcgata taccccgagt atgtaccgtc
      841 ttgaatacgt tgcaagtctt gcgtaacaag gtgcgtacgc cgtttcaaac taaaaattgt
      901 gcgcaaaaaa gtaaaaacgc cgatacaaaa aaataccccc gtaacggctg tcacgccgat
      961 tgcagcactg tcgcccaaaa ggtgtaattg catgcgctcg tttgttaaaa cacggttcac
     1021 ccaaaaaccg atatacccga ctaaaggcaa aagaaataaa agcgtcgaca ggattatttt
     1081 ttgcataatc gacgcgcggc ggacaaaatt tattttatcc agccacggaa agcgcaccac
     1141 gcggccttca ataagatgca cgccgcccgc cgcgacacgc ttcaagagta cgtccgcgtt
     1201 gcgcttttgc tcgtcggaca cggggtaacg cgccgaaaga taaccggttg tttggccgcc
     1261 ctcgacaatc ggcgaaacgt ttgcgagcac ccaatagtgg tcgccgtctt tacaacggtt
     1321 cttcagcacc tgcgcccacg gcttacccgc gcggatggta ctccataaat cggcaaagac
     1381 ggcgggaggt atttcggggt ggcggataac attgtgcggt tgaccgatga ggtcgtctac
     1441 cggcatttta gccgcttcgg caaagtcgcg gttggcataa gtgatgacac cttttagatt
     1501 tgttttcgtc accaactgcg cgtcgtcgcg ttggataaat tctctacccg ttacaggccc
     1561 attatttctc atacttactc cttcgtcctt taccaattaa atctataatg ccgggaacat
     1621 caataataaa cgccatttcg cgtgcgccca aaattgtcgc accgccgatg ccgcgcaagt
     1681 tggcgaggag tcctgtgagc ggtttgatca ccgtcttatg ctcgcccaac ggctcgtcga
     1741 cgacgatacc aaaacggcgc tcgccgtctt cgacgatcac taaactcgcg cgctcgctac
     1801 tgtcgtgttc gatattgaaa atactgctta aacttgtgta aggcaacgcc tcgccgcgca
     1861 aattaaaaat ctgattgcgt gtgtcgtcgt tcaatgcgtc ggtgctaaaa tcgaggcact
     1921 ctttgacgct ctccaacgga aagacaaatt tttgcgcgcc cacccgcacc acaaagccgt
     1981 cgatgatcgc aagcgtcaac ggcaacacaa tacgaagcgt catgccttgc ccaaacaccg
     2041 actggatgcg gatgactcca cgcagtgcgt cgatattttt tttaacgaca tccatgccga
     2101 cgccgcgacc agaaatttcc gtcaccgctt cggcggtaga aaaaccatgg gcaaaaataa
     2161 attgttgaat ttcgctttcg ctatattcac ggtcggtatc ggcaatccct ttcgcacgtg
     2221 ctttttctaa aattcgggat aaatccaacc cgcgcccgtc gtcggttacc tcaatgacaa
     2281 tcgctcccgc cgcgttgtac gcgctgagcg acaccacgcc ctcttggggc ttgccttgct
     2341 cgagccgtgc ctctacgctt tcaataccgt ggtcgaccga gttgcgcaca atgtgcatga
     2401 gaggatcgcc gatacgctcg atgacagatt tgtcaagctc ggtatcttcg cctttgagct
     2461 caagacgcac cattttaccc gagacacccg ctgcatcgcg caccacacgc tccatgcggt
     2521 ggaacgtgcg gccaatcggc accatgcgca agttcatcgc caactcgcgc acgttttcga
     2581 cgaggcgcgt catctctgaa acggggcgca taaattcttt cgtctgtgtt tcgcgcgaca
     2641 actgttgcag tgtaccgttt ttcatcacaa gctcgccgac aaggtcgacg agtaaatcaa
     2701 gcttatccga gctgacacgc aattgcttcg aagcctttgt ttgttcgctc tctacactgc
     2761 tcgcttttgt cgtattcgcg tttgaaggat ttttattttc atttgtggga tttaccccgc
     2821 tacccgcgcc cttttccgga acggtgtgaa ttgctttggc aatctgagtg atggaaaccc
     2881 gcgacagtaa aaactcaaac gcctcgttga gctctcttgc agcgtcttcg tgcgatactg
     2941 ttttttgaaa cgccacttca aaaactaaaa tcgcatcttc ggggtcaaaa ttttgtgccg
     3001 agggtaaatc cgtcgtgatg agttttatat attccagcga actatactta aacaagaaac
     3061 gcaacgcctt ctcgggggca aggccgtgac ggtaaagatc cgttttgagg cgcacaataa
     3121 aacgcgggtg cgtactttcg ttgaacacct ccgcttcctt tgtattttga ggtaaagcac
     3181 tcgtgtcgat gtctaccgct ttctcgctgg tatccatgag ccgccgcaac tgaagcaaga
     3241 ttgtgttact caccgagaca agatgcttga gcgcgctttc aggagtgtct gtggtgaaat
     3301 ggttaatgag cgctaccaga tgatcggagg cagtgatgag cagcgcggca atttcgagag
     3361 ttaccggcaa acgtttgttt ctcagggcat ctaaaagatc ttccacctcg tgcgagaagc
     3421 ataccaactc gtcgaagtcg aaaatgccac cagagccttt gatagtgtgc actgcacgaa
     3481 atgcagtgga gagcgcctca tcgtcggtta aatttttttc caacccaagc aacgcctctt
     3541 ccaacgagga caaaagctcc agcgcttctt ctctaaaagt ggcgcgtaaa gaatccatgc
     3601 tcaagtaaaa tgctgtattt cacgcccata gcggaagcga ttgttttgcg cgaccacaat
     3661 tttgtcgcgg aaaagcccca tcgcaccata aagctcgatc atatcgacga ccggcgcact
     3721 gtgacggatt aacttcactt ttttctgttg ggcttcagat tcttttttca acaccaacaa
     3781 cacttgaaag ccagcactgt cgatactttc gatgccggat aaatctaagg cgagtgcatc
     3841 gaatttatta aaaaattcca atagggtaaa aaacgcttct tttgcataac gaatagtaaa
     3901 attccccttc agagcaacga cacctcgacc ctcttgcgca gcaatttgta taattttaaa
     3961 cgttccctgt ggcattttta caaatttaat tcttgtttgt tttatttgtc aagtagataa
     4021 aaattataaa ctatagttaa cttaagagcc tgtgcaccag ctcgcgcaac gtgtacaaga
     4081 agagaatttt tttgtaaatt tttgtatgcg gttcgctcaa aacac
//
LOCUS       DAOMPD010000230         4120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_8997_length_4120_cov_2.776138, whole genome shotgun sequence.
ACCESSION   DAOMPD010000230 DAOMPD010000000
VERSION     DAOMPD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8997_length_4120_cov_2.776138"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLY93_08665"
     CDS             <1..466
                     /locus_tag="PLY93_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69589.1"
                     /translation="NAVILLLADFFLEILIFAGIAIVDVHLLGQGLVPLLSVFALGFI
                     WNFFCHFYFRPRILTMPYSFELGLINFGMLNGTSATGLMLLKMVDPHFKTPAVRVFAE
                     GSMLTQPFIAGGFITLLTPFIVTALPPWISIALYASLLVVWLIVGLSLGKRR"
     gene            complement(486..1625)
                     /locus_tag="PLY93_08670"
     CDS             complement(486..1625)
                     /locus_tag="PLY93_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRP69590.1"
                     /translation="MKQSYIHNDGESLISGTNLILFGLIIALVAFGLNILLASSSFMA
                     REQFSDPYFFLKKQAFAAFLGFLGMLAATQVDPNRYRQWVWPLYALAIFLLVLVFVPG
                     IGKRAGGANRWIVLGGLSLQPSDFAKVALILVLARIYSQAPVTTEREHGSIDWRLSFV
                     TLFIIALPTFLIAIEQDLGTAIHLVLVASTLLFFTRYPLIMQIGAFLVSVTAAGYIIL
                     KTPFRLGRISAWLNPWDERYGAGFQLVASMKSFLAGGLTGKGLGEELVRHHLQERHTD
                     FILAIVAEDLGALGVIALLAAYFLIAFYALILAARIRDDFMRLLATGCVLALILQVVI
                     NAGVTMGIFPTKGINLPLVSNGGTSLFVYMVMCGMMLSALRREQV"
     gene            complement(1637..2788)
                     /gene="prfA"
                     /locus_tag="PLY93_08675"
     CDS             complement(1637..2788)
                     /gene="prfA"
                     /locus_tag="PLY93_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRP69591.1"
                     /translation="MDGYTNHSCKDRWEYIHNFMAELNENNLRTIVANFAAIEERMAD
                     PQVTSDNKRLTELSRERARIGPAAELATEWLSLKNQIADCKKGLETEKDSEMKEILSS
                     ELSELCEKFEALSYELQLELIPKDVDAGKNIYLEIRAGTGGDEAGLFVRDLFRMYSRY
                     LDTLGIPYEIQDLSETGVGGFKEAILYVSGAKAYELLHFEAGTHRVQRIPATEANGRI
                     HTSAVTVAVIPEADEDEISINPNDLRIDVFRSSGAGGQHVNKTESAVRITHLPTGIVV
                     GCQEEKSQIKNRAKAMKALMSKLVEKQRQEHAEKYATEKKAQVGSGDRSEKIRTYNYP
                     QNRVTDHRINFTAHNLDQVMDGKLDAIIDALLLADREAKMREGEAAVAK"
     gene            complement(2805..2877)
                     /locus_tag="PLY93_08680"
     tRNA            complement(2805..2877)
                     /locus_tag="PLY93_08680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2841..2843),aa:Arg,seq:cct)
     gene            complement(2922..3443)
                     /locus_tag="PLY93_08685"
     CDS             complement(2922..3443)
                     /locus_tag="PLY93_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69592.1"
                     /translation="MKKKRHHHRNEKLGTSVAAQPSLKRINWKGESNVEVKYAQCCNP
                     SPPDSISGFISRLGVVSVHKKACPSLADLANRPEQAERIIPLTWVGHSTGRQATVEIV
                     GVDRPQIYLDIVKQLAESGANILEANATTTQVGKIHDLFVIEVDSNEHLTNILKEITT
                     VDGIESAEERFGG"
     gene            complement(3415..>4120)
                     /locus_tag="PLY93_08690"
     CDS             complement(3415..>4120)
                     /locus_tag="PLY93_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRP69593.1"
                     /translation="GVRVLVKEVQECYSVLGLVHTLWTPVPGRFKDYISVPKSNGYQS
                     LHTTVIVPGGQNIEVQIRTWAMHQISEFGIASHWAYKEKVATAKNYETQFFELQNMAI
                     SDEESDSKEFLQELVSNLSQTNEVYVISPKGKIFTMPENSTVLDFAFRIHTDIGLKCA
                     GAKIGERLVSIRTPLKNGDQVEIITNSSVKPSLNWLNFLKTPHARAKLKSYFRKIDTS
                     IAERLIENEKKAPPPP"
BASE COUNT         1005 a          994 c         1007 g         1114 t
ORIGIN      
        1 caacgccgtt attttactcc ttgccgattt ttttttagag atactcattt ttgcagggat
       61 tgccatcgtc gatgtacacc tcttgggaca aggattggtg ccgctattga gtgtgttcgc
      121 gcttggtttt atatggaatt ttttttgcca cttttatttt cgaccgcgca ttctcacgat
      181 gccgtatagc tttgagttgg gcctcatcaa ctttggtatg ttgaatggca catcggccac
      241 gggactcatg ttactcaaaa tggtcgatcc tcatttcaaa acgcctgcag tgcgtgtgtt
      301 tgcagaaggg tcgatgctca cacagccctt catcgcaggg ggattcatca cgctactgac
      361 accatttatt gtgacggcgt taccgccatg gatttccatc gctctttacg caagcttatt
      421 ggtagtgtgg cttattgtcg gcttgtcatt gggtaaaagg cggtaagagc gcggtaaaat
      481 tatttttaca cctgctctcg acgcagtgcg ctgagcatca tcccacacat caccatatag
      541 acaaaaagcg aagtgccccc gttggaaact aacggcaggt tgattccctt tgtggggaaa
      601 atccccatcg ttacacccgc gttgataaca acttgtaaaa taagcgctaa cacacatccc
      661 gtggctaaaa ggcgcatgaa atcgtcgcgt atacgtgcgg ccaagatgag agcataaaac
      721 gcaatcaaaa aatacgcagc caataacgca atcactccca acgcacccaa atcttcggcg
      781 acgatggcca aaataaaatc ggtgtgccgt tcttgcaaat gatggcgcac caactcttca
      841 ccgagcccct tgccagttaa accgcctgct aaaaacgatt tcatcgatgc gacaagctga
      901 aagcccgcgc catagcgttc atcccaagga ttgagccatg cggatatacg acccaaccga
      961 aaaggcgttt tgaggataat gtaacccgcc gccgtcacgc tgactaaaaa tgcgccgatt
     1021 tgcataataa gaggatagcg agtaaaaaag agaagtgtgc ttgcaactaa cacgagatgg
     1081 atggcagtac ctaagtcttg ttcaatggcg atgagaaaag tggggagcgc gataatgaaa
     1141 agagtcacaa acgaaagccg ccagtcgatg cttccgtgtt cgcgttcggt tgtaacaggg
     1201 gcttgcgaat agatgcgagc aagcacgagg atgagggcta ctttagcaaa atcggaaggc
     1261 tgcagggaaa gcccccccaa cacaatccat cggttagcgc cacccgcccg tttaccgatg
     1321 ccgggcacga acacgaggac gagaagaaaa atcgctaacg cataaagtgg ccacacccat
     1381 tggcggtagc ggttgggatc gacttgtgtg gcggcgagca tccccaaaaa acccagaaag
     1441 gcggcgaacg cttgcttttt caaaaagaaa taagggtcgc taaattgctc gcgcgccata
     1501 aacgacgacg acgcaagaag aatgtttaat ccaaacgcaa cgagagcaat gatgagcccg
     1561 aataaaatta aattcgtgcc gctaatcaaa gactcgccgt cgttatggat ataactctgc
     1621 ttcattgtaa tgcgagctac ttcgcgacag cggcctctcc ttcacgcatt ttagcttcgc
     1681 ggtcggccaa gagaagggcg tcgataatgg catcgagctt gccatccatc acttggtcga
     1741 ggttatgtgc ggtaaaattg atgcggtggt cggtgacgcg gttctgcgga tagttatatg
     1801 tgcggatttt ttcgctgcga tcccccgaac ccacttgcgc ttttttttcg gtggcatatt
     1861 tttcggcatg ttcttgccgt tgtttctcca ccagtttcga cataagcgct ttcatcgctt
     1921 tggctcggtt tttaatctgc gatttttctt cttggcaacc gacgacaatg cctgtgggta
     1981 agtgggtaat ccgcactgct gattcggttt tgttgacgtg ctggccaccc gcacccgacg
     2041 agcgaaacac atcgatgcgc aaatcgttgg gattgatact gatttcatct tcgtcggctt
     2101 cgggaatcac tgccaccgtt acggcggatg tatggatgcg cccgttagcc tccgtcgcag
     2161 ggatgcgctg cactcggtgg gtacctgctt caaaatgcaa cagctcgtat gctttagcgc
     2221 cggagacgta gagaatcgct tctttgaaac caccgactcc tgtttcgctg aggtcttgaa
     2281 tttcatacgg gatacccaat gtgtcgaggt agcggctgta catacggaac aaatcgcgca
     2341 caaaaagtcc cgcttcgtcg ccaccggtac ccgcgcgaat ttcgagataa atatttttac
     2401 ccgcatcgac atctttaggg atgagttcaa gttgcaactc gtacgagagt gcttcaaatt
     2461 tttcacatag ttcggaaagt tcgctggaga gaatttcctt catctcgcta tctttttcgg
     2521 tttcgagacc ttttttacaa tcggcaattt ggtttttgag agaaagccac tcagtggcta
     2581 attcggcggc agggccaatg cgtgctcttt cgcgcgaaag ctcggtgagt cgtttattgt
     2641 cgctggtaac ttgtgggtcg gccatacgct cttcgattgc cgcaaagtta gcgacaatgg
     2701 tgcgtagatt attctcattc aattctgcca taaaattatg aatatactcc cacctatctt
     2761 tgcatgaatg gttggtgtag ccatccattc atgcatctcg tttctgctcc cacccggagt
     2821 cgaactgggg tcttcgcctt aggagggcgc tgctctatcc gtctgagcta taggagcctc
     2881 aagtgcgctg aagtgaataa aagagtgagg cggacaagct cttaaccacc gaatcgttct
     2941 tctgccgatt caataccgtc gacggtggtg atttctttaa gaatatttgt taagtgttcg
     3001 ttggagtcca cctcaatcac aaataggtcg tggatttttc ccacttgcgt cgtggttgca
     3061 ttggcttcga gaatgttagc gccgctttca gcgagttgtt ttacaatatc caagtaaatt
     3121 tgaggacgat ccacgcctac aatttcaacg gtcgcttgcc tacccgtcga gtgccccacc
     3181 catgttaaag gaataatgcg ctccgcttgt tcggggcggt ttgctaaatc ggcaagcgaa
     3241 gggcacgctt tcttatgtac tgaaacaaca cccaaccgag agataaatcc tgaaattgag
     3301 tcgggggggg atggattgca acactgtgca tatttcactt cgacattgct ttcacccttc
     3361 caattgattc tcttgagcga aggttgcgcg gcgacgcttg tccccaattt ttcattacgg
     3421 tggtggtggc gctttttttt cattttcaat aaggcgttcg gcaatcgaag tgtcgatctt
     3481 tctaaaataa cttttgagtt ttgcgcgggc atgcggtgtc ttgaggaagt tgagccagtt
     3541 taaagatggt ttaacgctac tgtttgtgat aatttcgacc tgatcgccgt ttttaagtgg
     3601 cgtgcggata ctcacgaggc gttcgccgat tttcgctccc gcgcatttca agccaatgtc
     3661 ggtgtggatg cggaatgcaa agtcgagcac ggtcgagttc tccggcattg taaaaatttt
     3721 gcccttcgga gaaatgacat aaacttcatt cgtttggctg aggtttgaaa ccaactcttg
     3781 taaaaactct ttcgaatcgg attcttcgtc ggagattgcc atgttttgta gctcaaaaaa
     3841 ctgtgtttca taattttttg cagtggctac tttttcctta tacgcccagt gcgaagcgat
     3901 accaaattca gaaatttggt gcatcgccca agtacggatt tgcacttcga tattctgacc
     3961 tccgggaacg attactgtgg tgtgcaggct ttgatagccg ttcgacttag gtacagagat
     4021 ataatctttg aagcggccag gtaccggtgt ccataacgta tgcacaagcc ccaaaaccga
     4081 ataacactcc tgtacttctt tcacaagaac ccgcacgccg
//
LOCUS       DAOMPD010000231         4108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9031_length_4108_cov_2.151739, whole genome shotgun sequence.
ACCESSION   DAOMPD010000231 DAOMPD010000000
VERSION     DAOMPD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9031_length_4108_cov_2.151739"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..543
                     /locus_tag="PLY93_08695"
     CDS             118..543
                     /locus_tag="PLY93_08695"
                     /inference="COORDINATES: protein motif:HMM:NF039584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69594.1"
                     /translation="MWGSEEYDDEIITHLEELRTLQDQTHTVLSFVPWTFQAQTKGFK
                     LRKVPPHEYLKMVALARLFFDNIPHIEVSIMVAGKQLGELALHFGADDINSPVIEENV
                     LRSFGLKSVAESQQFIENAGFTPVRRSLNFIRGEKTDLG"
     gene            580..1371
                     /locus_tag="PLY93_08700"
     CDS             580..1371
                     /locus_tag="PLY93_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRP69595.1"
                     /translation="MAYTHLKVEQKTTTLKSSLEAHYAVVTIDAPPMNPLSSKVFHDF
                     DALIDSLESNKIRVAIITGNGKAFVAGADISEMKGMSVEKAKAYSLLGHKIFSRLENS
                     PVVFIAAVNGFALGGGLELALASDIRLLSEAAQLALPETSLGLIPGFGGTQRLARLIG
                     MSAAKYLSLTTERIGAADALRVGLAAKVVPAEKLIEEAESMACKILSLGPNAVSLLKK
                     VITHGADKSLPDALNYEAEEFSKLFAGTQAQEGLTAFLEKRPAKF"
     gene            1382..2053
                     /locus_tag="PLY93_08705"
     CDS             1382..2053
                     /locus_tag="PLY93_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69596.1"
                     /translation="MALKKKKKEKEVLPSSLLDNATAKELAVVEQVRKIQVERLRETY
                     SDFFKDPEYRKIAQYFFEQIYGAGDKASRDAAFNKIYEKLSAAVNAKRIVRVTQLKQL
                     NELTDELDLKIAQEFLRSNKKRAMTLKDFEVCYKALNNKEERTIQLQLLMDTTRFFHK
                     LAHIPLLGFVIKPTQLAAKILGVEYIMDFFMEGYRSFKSVKNAEPFMQAIEDREKAYI
                     QKFFP"
     gene            2067..2849
                     /locus_tag="PLY93_08710"
     CDS             2067..2849
                     /locus_tag="PLY93_08710"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRP69597.1"
                     /translation="MCEILGMSANVPTDIVFSFTGLIRRDGDTGPHKDGWGIAFYEDK
                     GVRLFHDPEESSRSKIAAFLQSYPIRSETVVSHIRKANRGKVALKNTHPFMRELWGRN
                     WVFAHNGQLKGIKKEKMESFQSVGSTDSEAAFCWLLGKLRRKFKNPPDNKTLIHEVER
                     LFAELAERGVANILMSDSSFLFTHCSNRLVYITRRAPFSRAQLLDADVTVDFNEATTP
                     KDVVTILATRPLTGNEDWNNYAPREYRVWRRGETVYQSRNAF"
     gene            complement(2846..>3697)
                     /locus_tag="PLY93_08715"
     CDS             complement(2846..>3697)
                     /locus_tag="PLY93_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69598.1"
                     /translation="FNAREIASRLEHILNQEKIAFDKEALLPIAERGEGSLRDAISLL
                     DQTLAFSGQGTLTMAAVRESLDILPLEVYNKFAAGLRQNDIHLLLNSIHELSQSGANL
                     RVFRNDLLSYMRTMVLLSEGVEVYSLSKEEAEQIHARAAEWDRATLIRVFQQLFRLNQ
                     EFGQMLSAKASELKMSLEIALVDLVARLKEPSVSQLVQKIERLKTAIEKGEKFEDAPL
                     LTQVQSASIAVKGKPSVSSVPENSGVKKTETNEEKLDGDASTLIQKAFLAEEVPVSPE
                     TQKMFEN"
     assembly_gap    3698..3712
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3713..>4108)
                     /gene="dnaX"
                     /locus_tag="PLY93_08720"
     CDS             complement(<3713..>4108)
                     /gene="dnaX"
                     /locus_tag="PLY93_08720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRP69599.1"
                     /translation="AYLFHGARGVGKTTMARILAKALNCADGPTSSICNTCEHCLEIT
                     EGRSTDVIEIDAASNRGIDNIRELRAQAVFAPMKSRYKVYIIDEVHMLTTEAFNALLK
                     TLEEPPNHVVFIMATTELHKVPETIVSRCQ"
BASE COUNT         1111 a         1028 c          936 g         1018 t
ORIGIN      
        1 ttatcgaatt gagaataatt gatagcttaa atttttaaac acatcaaatc aaagactaaa
       61 ccaacatgag attcctcaag aataaaatcc tcttggttca aaagctcgac aaacatcgtg
      121 tgggggagcg aagagtacga cgatgaaatc attacgcatc tggaagagtt gcgcacactg
      181 caagaccaaa cacacactgt actttcattt gtgccgtgga ctttccaagc gcagacgaaa
      241 ggctttaagc tacgcaaagt ccccccacac gaatacttaa aaatggttgc acttgcgcgc
      301 cttttcttcg acaacattcc gcatatcgaa gtttcgatta tggtcgcggg aaagcagttg
      361 ggtgagctgg cgctccactt tggcgcagac gatatcaact caccggttat cgaagaaaac
      421 gtgctgcgtt cctttggcct taaaagcgtc gccgaatcgc aacaattcat cgaaaacgcc
      481 ggatttaccc cggtacggcg cagtttgaac tttattcggg gagaaaaaac cgacttgggt
      541 taatgttgac agtgggtttc attatccttt tttcccgtga tggcatacac ccacctcaaa
      601 gttgaacaaa aaacaacgac gctcaagtct tctctcgaag cacactacgc tgtcgtgaca
      661 atcgacgctc cccccatgaa tcccctctcg tcaaaggtct tccacgactt tgacgcgctc
      721 atcgactcgc ttgagtccaa taaaatccgt gtcgccatta tcaccggcaa tggcaaagcg
      781 tttgtcgctg gggctgacat tagcgaaatg aaaggcatgt cggtggaaaa agcgaaggcg
      841 tattctctct tggggcacaa aattttttcg cgtttagaaa actctcccgt tgttttcatc
      901 gctgcggtaa acggttttgc gttaggcggc ggtcttgaat tagcattggc atcagacatc
      961 cgccttctct ccgaggctgc acagctcgcg cttcccgaga cgtcgttggg gcttatcccc
     1021 ggctttggcg ggacgcaacg cctcgcgcgc ctcattggaa tgagcgccgc aaagtattta
     1081 tctttaacca ccgaacgcat cggcgcagca gacgcactcc gggtaggcct tgctgcgaaa
     1141 gtcgttcccg cagaaaagct catcgaagaa gccgaaagca tggcgtgcaa aattttgtct
     1201 cttgggccaa atgccgtctc tcttcttaaa aaagtcatca cacacggtgc cgataaatca
     1261 ttacccgatg cgctcaatta cgaagccgaa gaatttagca aactttttgc agggacacaa
     1321 gcgcaagaag gattaaccgc gtttctggaa aaacgccccg caaaatttta agaaaagccg
     1381 tatggcgctc aaaaaaaaga aaaaagaaaa agaagtgctc ccctcttcac ttttagacaa
     1441 cgccaccgcc aaagagcttg ccgttgtcga acaggtgcgt aaaatacaag tggagcgtct
     1501 acgcgaaacg tacagcgact ttttcaaaga ccccgaatac agaaaaattg cccaatactt
     1561 ttttgagcaa atttacggcg caggtgataa agccagccgt gacgcagcgt ttaataaaat
     1621 ttacgaaaaa ttatcggctg cagtgaatgc gaaacgcatc gttcgggtca ctcaacttaa
     1681 acagctcaac gagctcaccg acgaattgga tttaaaaatt gcgcaggaat ttttgcgttc
     1741 gaataagaag cgcgctatga ctctcaaaga ttttgaggtc tgttacaaag cgctgaacaa
     1801 caaagaagag cgcacaatac aactgcagtt actcatggat accacgcgtt tttttcataa
     1861 attagcgcac ataccgcttt taggtttcgt gattaaaccg acgcaactgg cggcaaaaat
     1921 actgggcgtc gaatacatta tggacttttt catggaaggc taccgttctt tcaaatcggt
     1981 gaaaaacgcc gaaccgttta tgcaagcaat cgaagataga gaaaaagcgt acatccaaaa
     2041 attttttcca taaagaatta cccaccatgt gtgaaatttt aggcatgagc gccaatgtgc
     2101 ccaccgacat tgtgtttagt tttacagggc taattcgccg tgatggcgac acaggcccgc
     2161 acaaagacgg ttggggcatc gctttttatg aagacaaagg ggtgcggctt ttccacgacc
     2221 ccgaagagag tagccgctct aaaattgcgg cgttcttaca gtcatacccc atccgcagcg
     2281 aaaccgtcgt tagccatatt cgcaaagcca accgcggtaa agtggctctc aagaacacgc
     2341 accccttcat gcgcgaactc tggggccgca attgggtttt tgcgcacaac ggccaactga
     2401 aaggcatcaa aaaagaaaaa atggagagtt tccagagcgt cggctcaacc gatagcgagg
     2461 cggcgttctg ttggctcttg ggtaaattgc gccgcaaatt caaaaatcca cccgataaca
     2521 aaactctcat tcacgaagtg gaacgtcttt ttgcagagtt ggctgaacgc ggtgtggcca
     2581 atatcctcat gagcgattcg agttttcttt ttacccactg ctcgaaccgg ttagtgtata
     2641 tcacacgccg cgcacctttc agccgtgcgc agcttttaga cgccgatgtc accgtcgact
     2701 ttaacgaagc caccacaccc aaagacgtgg tgacgatttt agcgacgcga cctttaaccg
     2761 gtaacgaaga ctggaataat tatgcaccgc gcgaataccg cgtgtggcgc agaggcgaaa
     2821 cagtttatca aagccgcaac gctttttaat tttcaaacat tttttgagtt tcgggcgaca
     2881 ctggtacttc ttcggcgagg aatgcttttt gaatcaatgt tgacgcgtcg ccatccagct
     2941 tctcttcatt cgtttcagtt ttttttacgc cgctattttc gggtacggat gacactgacg
     3001 gttttccttt taccgctatc gatgcgcttt gaacttgagt taataacgga gcatcttcaa
     3061 atttttcgcc tttttcaatc gccgttttta accgctcaat tttttgcaca agctgactta
     3121 ccgacggctc tttgagacgc gctactaaat cgacgagtgc aatttctaaa gacattttaa
     3181 gttcgctcgc ttttgcagaa agcatttgcc caaactcttg attgagccga aacaattgct
     3241 gaaaaacgcg tatcaacgtt gctctatccc actcggcggc gcgcgcgtgg atttgctccg
     3301 cttcctcttt cgagagcgaa tagacttcga ctccctcgga caagagcacc atcgtgcgca
     3361 tatacgaaag taaatcgtta cgaaacacgc gcaagttcgc gcccgattgc gaaagctcgt
     3421 ggattgaatt taagagtaag tgtatatcat tttgtcgcaa gcccgccgca aatttattat
     3481 aaacctccag cggcaatatg tcgagcgact cgcgcaccgc cgccatcgtg agtgtcccct
     3541 ggcctgaaaa agcaagcgtt tggtctaaaa gtgagatggc atcgcgcaac gaaccttcac
     3601 cgcgttcggc aattggcaag agcgcttctt tatcgaaagc aattttttct tgattgagaa
     3661 tgtgctccaa gcgggaagcg atttcacgcg cgttaaannn nnnnnnnnnn nnttggcaac
     3721 gcgacacaat cgtctcgggt actttgtgca gctcggtcgt cgccataata aaaacaacat
     3781 ggttgggagg ttcttctaac gttttcaaca gtgcgttaaa cgcctcggtc gtgagcatgt
     3841 gtacttcatc gatgatatac actttatagc gcgacttcat cggtgcaaaa accgcctgcg
     3901 cccgcagttc acggatattg tcgatgccac gattcgacgc cgcgtcgatt tcaatcacgt
     3961 cagtggaacg cccttcggtg atttctaaac aatgctcgca ggtgttacat atactcgagg
     4021 tagggccatc ggcacagttg agcgccttgg ctaaaatacg cgccatcgtg gtttttccca
     4081 caccgcgtgc accgtggaaa aggtaagc
//
LOCUS       DAOMPD010000232         4101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9044_length_4101_cov_2.810677, whole genome shotgun sequence.
ACCESSION   DAOMPD010000232 DAOMPD010000000
VERSION     DAOMPD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9044_length_4101_cov_2.810677"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLY93_08725"
     CDS             complement(<1..438)
                     /locus_tag="PLY93_08725"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRP69600.1"
                     /translation="MITDDVRIDTLKPLVPPAILIEEIPLPYAGQTFVKAARDQINAI
                     VKGKDDRLIVVVGPCSIHDIKAAHEYATLLKPLRDKYKEELEIVMRVYFEKPRTRSGW
                     KGLINDPHIDNSFQINEGLRLARQLLVDLTMQGLPAGCEFLDTI"
     gene            630..917
                     /locus_tag="PLY93_08730"
     CDS             630..917
                     /locus_tag="PLY93_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRP69601.1"
                     /translation="MAIKPLADRVLIEAAPEKEEKIGSIFIPDTAKEKPNVGVIVAAG
                     PGKVTDDGKTVPLQVKAGQKVLYGKYSGTEIKENGKEYLIVRESDILAIVE"
     gene            946..2586
                     /gene="groL"
                     /locus_tag="PLY93_08735"
     CDS             946..2586
                     /gene="groL"
                     /locus_tag="PLY93_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRP69602.1"
                     /translation="MAKMLEYDESARAKLMTGINKLANAVKVTLGPRGRNVVIDKKFG
                     APTITKDGVTVAKEIELEDAYENMGAQLVKEVSTKTNDVAGDGTTTATILAQAIAREG
                     IKNVTAGANPMDLKRGIDKAVEVVVEHVKKNAKKVESKDIAAVATISANNDPEIGKLI
                     ADAMDKVGKDGVITVEEAKSIETSLDTVEGMQFDRGYVSSYMVTDAENMTATLENAYV
                     LIYDKKISAVKDVAPILNQVAQGGKALLIIAEDLEGEALATIVYNTIRKAINCVAVKA
                     PGFGDRRKAMLEDIAILTGGHVISEELGQKLESATLESLGKIEKVIVEKENTTIIKGG
                     GKDSDIQHRIKQIKKQIEETTSDYDREKLQERLAKLAGGVAVIRVGAATEVEMKEKKA
                     RVEDALSATRSAVEEGIVAGGGLTLLKAIPIVEKLKLEGDQETGKAIVARALEEPLRQ
                     IAANSGMEGSLIVQQAKGEKENVSFNAATLQWEDMLKAGIIDPAKVTRSALQNAASIA
                     GLVLTTECLIADKPEKKDPMAGGMPPGGMGGMGGMGDF"
     gene            2849..4009
                     /gene="hflX"
                     /locus_tag="PLY93_08740"
     CDS             2849..4009
                     /gene="hflX"
                     /locus_tag="PLY93_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRP69603.1"
                     /translation="MDASSLYQQKLESKEQYRALCVGQYGSTYFPHKRSQEESLRELL
                     MLAETAGVRTLHELLLHHTDIHPANFFTQGQLERIEKVIHDFNLNLLFIDAQLKPNQL
                     RNLEEIFKIRVLGRIEVILDIFAMRAKTREAILQVELAQLLYIMPRLKGLGGVLSRQG
                     ATGAPGIGTRGPGETMLETDRRYIRRRIQKIRDDLKHVERDRATSRHGRNLTTFALVG
                     YTNVGKSSTLNALSSSSQRVFAEDKLFATLDTASRKIYLGEKDFKPEYAILTDTVGFI
                     RNLPASLVAAFQSTLSEIQYADVIIEIHDASSAQLADEMAVVENELKRLNVADKPRLL
                     YFNKSDLVFPEEKKRLKDEYPQAVWGNSLSKEGVAELRKRLSTKEKLNIDCR"
BASE COUNT         1213 a          933 c         1007 g          948 t
ORIGIN      
        1 aattgtgtct aaaaattcac accccgccgg cagaccttgc atggttaaat cgacgaggag
       61 ctgacgcgcc aagcgtagcc cttcgttgat ttggaacgag ttgtcgatgt gagggtcgtt
      121 gatgagccct ttccaaccgg agcgtgtgcg tggtttctca aaatacaccc gcatgacgat
      181 ttctaattct tctttatatt tgtcgcgcag cggctttaaa agcgtggcgt actcgtgtgc
      241 agctttgatg tcgtggatag agcaggggcc gaccacgacg atcaagcggt cgtctttgcc
      301 tttcacgatg gcgttaattt gatcgcgcgc ggccttcaca aaagtttgac ctgcgtaagg
      361 gaggggaatc tcttcaatga ggattgccgg tggtacaagg ggtttgagtg tgtcaatacg
      421 gacgtcgtct gttatcataa tcttttatcg gttaaatgta gtttatttta tgtaaaattt
      481 caacaattat ttttgccatt ttcaataatt atagtatatt agcagcatag caaaaagagt
      541 gctaacacaa cgcatggcga gtgatagcgt tttttattgc gagtgctaag gtgatccctt
      601 ggctcaaaat tttggaggct aaaaaatata tggcaattaa accattggca gaccgcgttc
      661 ttatcgaagc ggcccctgaa aaagaagaaa aaatcggtag catctttata cccgacacgg
      721 caaaagaaaa gccaaacgtc ggcgtgattg tggctgctgg ccctggcaaa gtcaccgacg
      781 acggtaaaac tgtgccgttg caagtaaaag cagggcaaaa agtgctctat ggaaaatata
      841 gcggcactga aattaaagaa aatggcaaag aatatctcat cgtgagagaa tcggacattc
      901 tcgcgattgt agaataatta tagagttagt aaggtaggta aaattatggc aaaaatgcta
      961 gaatacgacg aaagcgcgcg tgcaaagctc atgacgggca tcaataagct cgccaatgca
     1021 gtcaaagtaa cgctggggcc acgcgggcgt aatgtggtta tcgataaaaa atttggcgct
     1081 cccaccatca caaaagacgg tgtcacggta gctaaagaaa tcgaactcga agacgcgtac
     1141 gaaaacatgg gcgcacagct cgtgaaagaa gtttctacaa aaacgaatga cgttgcaggc
     1201 gatggtacca ccaccgcgac aatccttgcg caagcgattg cacgcgaagg aattaaaaac
     1261 gtgactgcgg gtgcaaatcc catggactta aaacgcggta tcgacaaagc tgtcgaagtg
     1321 gttgtggaac acgttaaaaa gaacgcaaaa aaagtagaga gcaaagacat cgctgcagtg
     1381 gcgactattt ctgccaacaa cgatcctgaa attggtaagc tcatcgccga cgcgatggat
     1441 aaagtgggta aagacggcgt gatcaccgtg gaagaggcca agtcaattga aacatcgctc
     1501 gacactgtcg aaggtatgca attcgaccgt ggctatgttt cctcttacat ggtcaccgac
     1561 gctgaaaaca tgactgcaac gcttgaaaac gcgtacgtgt tgatttatga taaaaaaatc
     1621 agtgcggtca aagatgtagc tcccattttg aaccaagttg ctcaaggggg taaagcgctc
     1681 cttatcatcg ctgaagacct cgaaggcgaa gcgttggcga cgattgtgta taacaccatc
     1741 cgcaaagcga tcaactgcgt cgcagtcaaa gctcctggtt ttggcgaccg ccgtaaagcg
     1801 atgcttgaag acattgccat cttaactggc gggcatgtta tttcagaaga gcttggccaa
     1861 aaactcgaaa gcgcaacgct cgagagctta ggtaaaatcg aaaaagtaat tgttgaaaaa
     1921 gaaaacacca ccatcatcaa aggtggcggt aaagattctg atattcaaca ccgcattaaa
     1981 caaatcaaaa agcaaatcga agaaaccact tccgattacg atcgtgaaaa actgcaagag
     2041 cgtctcgcaa aactcgcagg tggcgtagcg gtcatccgcg tcggcgcggc aaccgaagtc
     2101 gaaatgaaag agaaaaaagc acgcgtcgaa gacgcacttt cggcaacgcg ttcggcagtg
     2161 gaagaaggta tcgtcgctgg cggtggattg acactcttga aagcaatccc cattgtagaa
     2221 aaactcaaac ttgaaggcga tcaagaaacc ggcaaagcga tagtcgctcg tgcgctcgaa
     2281 gagccactgc gccaaatcgc cgcaaactcc ggcatggaag gttcgctcat cgtgcaacaa
     2341 gccaaaggcg aaaaggaaaa cgttagcttt aacgcggcga cactccagtg ggaagatatg
     2401 ctcaaagcgg gcatcattga cccggcgaag gtaacgcgct ctgcgcttca aaacgcggcg
     2461 tcgatagccg gtcttgtact cactaccgag tgcctcatcg ctgacaagcc tgagaaaaaa
     2521 gacccaatgg caggcggcat gcctcctggc ggtatgggtg gcatgggcgg tatgggcgac
     2581 ttctaagagt tgtttcaacg ccaaaaggct gtctaagggc tgcgccaagc gggccatgag
     2641 acagcctaaa aaaccgaaaa ttagcttatt ttgcgcggta cgcgcgcaaa ataagcgttc
     2701 aattatcttt tttagacatc tcttttctgc gcgctaatca ccaaagtagc cgcaacgatt
     2761 gacgttatac aaaaaaatgt atagattaac attatgctaa cacaaattcc aaagagtcta
     2821 tagctgttat tttgtagcct tttttacaat ggacgcctcg tcgctttatc aacaaaaact
     2881 cgaatcgaaa gagcaatacc gcgcactttg cgtggggcaa tacggctcga cctattttcc
     2941 ccacaaacgt agccaagaag agtcgctgcg cgaacttttg atgttggctg aaacagcggg
     3001 ggtgaggact ctccacgagt tacttttaca ccacaccgac atccatccgg cgaatttttt
     3061 tacgcaaggc cagttagagc gcattgagaa agtcatccat gattttaatt tgaatttgct
     3121 ttttatcgat gctcagttaa agccgaacca actacgtaat ttagaagaaa ttttcaaaat
     3181 tcgcgtgctg ggacgcatcg aggtcatcct cgatatcttt gcgatgcgcg ctaaaacccg
     3241 cgaagccatt ttacaagtag agcttgcgca gcttctttat atcatgccgc gtcttaaagg
     3301 tttgggcggc gtactctcgc ggcaaggcgc cactggtgcg cctggcatcg gcacgcgcgg
     3361 gcctggggaa acgatgctcg aaactgaccg ccgctatatc cgccgccgca tccaaaaaat
     3421 ccgcgacgac ttgaagcatg tcgagcgcga ccgtgccacc agccgtcatg gcagaaattt
     3481 aacgacattc gctttggtgg gatacactaa cgtgggcaaa agcagcactt tgaatgcact
     3541 ttcgtcgagc tcgcagcggg tttttgccga ggacaaactt tttgcaactt tggataccgc
     3601 gtcgcgcaaa atttatttgg gtgaaaaaga tttcaaaccc gaatacgcta ttttaaccga
     3661 cacagtggga tttatccgca atctacccgc atcgctggtg gcggcgtttc aatcgacgct
     3721 atctgaaatt caatacgccg atgtgattat cgaaatccac gacgcttcct cggcgcaact
     3781 tgccgatgaa atggcggttg tcgagaacga acttaaaagg ttgaacgtcg ccgataaacc
     3841 acgccttctt tattttaata aaagcgattt agtttttccc gaagaaaaaa agcgccttaa
     3901 agacgaatac cctcaagcag tgtgggggaa ttctctctca aaagagggcg tagcggagct
     3961 acggaaaaga ttaagtacta aagaaaaatt aaatatagac tgtcgctaat ccaaatttta
     4021 acttattacg ggattagaaa agaagcaatc gcaccgggtc tttagtcgct tgcttaactt
     4081 tcactaaaaa cgtcaccgcc t
//
LOCUS       DAOMPD010000233         4097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9059_length_4097_cov_2.730579, whole genome shotgun sequence.
ACCESSION   DAOMPD010000233 DAOMPD010000000
VERSION     DAOMPD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9059_length_4097_cov_2.730579"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLY93_08745"
     CDS             complement(<1..1137)
                     /locus_tag="PLY93_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HRP69604.1"
                     /translation="MNERVDESLAPKESASVAAKDFRCPNCGGPVHLALPGKSQSVRC
                     GHCGSILEPTHDVLLLRQKYEKAFSHKQWIPLSQEGVLKGIKFKCVGIVVRKDNYGGS
                     WTEYLIFNPYHGYRYLVESSGHWTYVEQLANVGYTPNNEPAWDGFSLKINFAGKKFKY
                     ATGYKATVKNVIGEFPWEVIVGEKNAVTEYLNPPYQATCERVEGMRDGKSYTTESNWS
                     LGEYVYPKEIQQAFNLEEMPPRMGFGMCEPNPYKKRRFFALLWSAALFFFASATCVIS
                     SNRSENAKVIDKVLPLNIANFELKKDGTTEYLEFDHEEGTIQLSKETNIEFEFSIPLS
                     QQWISLNVFLIDEVTGKLRHDRWKDKGTGRWTGKVFIAIDPGEGS"
     gene            complement(1134..1811)
                     /locus_tag="PLY93_08750"
     CDS             complement(1134..1811)
                     /locus_tag="PLY93_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HRP69605.1"
                     /translation="MKKSQCPSCGAPLIFENAHSLYAVCKYCRTMSVQSEDALKEVGK
                     TAALVEDGSPLDLGTTGTIDGHSFKIIGRIQYNFGLGFWNEWFLSIADDAGGPAWLGE
                     ANGTYFFTRQLTKSSLRDDLQFASLEAGMPLYIDDNKFFVKDIQKGKVVSGEGELPFS
                     FTGEYEAPVVDCVNAKDEFATIDFSEEKPIVFLGKILRFADLRLSRVRSVYGFKAEDA
                     GIQGLTA"
     gene            1987..>2847
                     /locus_tag="PLY93_08755"
     CDS             1987..>2847
                     /locus_tag="PLY93_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRP69606.1"
                     /translation="MFQSILRFFRSKKSLTADEILRYPKPRQYTREQHNLRIKDIDPD
                     AVKTVQRIQQMGCRAYIVGGSIRDLLLGRKPKDYDIVTDAHPQELRRMFANSRIIGKR
                     FRLVHVVFKGHKIIEVSTARSLPSNRAAAKNSDELYLKRDNQFGTFKDDAARRDFTIN
                     ALIFDIKNEAIIDYAGGFEDIQNRVVRVIGNPDISFPEDPVRMYRAVKFAALLGLDLD
                     AATYKAIQKYKNLLKKASNARLHEEYNKIFRSGKTALVFASLNNSGLMETLMPSIQQA
                     EGLHNRLTDEA"
     assembly_gap    2848..2857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2858..3493
                     /locus_tag="PLY93_08760"
     CDS             <2858..3493
                     /locus_tag="PLY93_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69607.1"
                     /translation="PLGRRLAIADRMIQEHEEVNLNIYYALLAASSLTNILQQNRRDS
                     EKQLEHKVKGPLSIVQKDIQLTRREYETLLQIFSTQNYFHRDVNDRKGWVKEFQQRKI
                     FPESFTVYKILARADDDEAALQKALFWEIGLRHHLPESIRKSAVERAQIDEKEYNAYT
                     PTKEDTRENKNTSNQRTPSPKDKNRRRRGRRGGNGRNKQKMPPALPPSPVP"
     gene            3497..>4097
                     /locus_tag="PLY93_08765"
     CDS             3497..>4097
                     /locus_tag="PLY93_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69608.1"
                     /translation="MHLFVFYIFISALFISSIFSTPSPEKKKLAITTPKAFIDFHKKA
                     IYTRLKKSIQKADVVFPPHKIRLAVFKKEKKVELWMPNVANQWRFVKEYAFTANSGES
                     GPKLYYGDLQIPEGIYGIDSMALSTEYHLALHVNYPNDFDKAMLALEGRDPHYVSTGI
                     NIHGGNISYGCVVVGNTNIEEIFYLTYLAGKNNTELYIFP"
BASE COUNT         1201 a         1018 c          821 g         1047 t
ORIGIN      
        1 gctgccctcg cccggatcga tggcgatgaa caccttgccg gtccagcggc cagtgccttt
       61 gtccttccag cggtcgtgac ggagcttgcc ggtgacttcg tcgatgagga acacatttaa
      121 acttatccac tgttggctga gtgggatcga aaactcaaac tcgatattcg tttctttaga
      181 aagttgaatt gtaccctctt catggtcaaa ttcaagatac tcggttgtac cgtctttttt
      241 tagttcaaaa ttcgcgatgt tgagagggag taccttatct ataacctttg cattttcgga
      301 acgattagaa gaaataacac aagtggccga agcaaaaaag aaaagcgcag cggaccaaag
      361 gagagcaaag aaacggcgtt ttttatacgg attgggctcg cacataccaa aacccatacg
      421 aggcggcatt tcttccagat taaacgcttg ttgaatttct ttgggataaa cgtattcacc
      481 caaagaccag ttactctcgg ttgtgtaact ttttccgtca cgcatccctt cgacgcgttc
      541 gcaagtggct tggtaaggcg gattgagata ctcagtgacg gcattttttt cacccacgat
      601 gacttcccag gggaactcac caatcacatt tttaaccgtc gctttgtacc cagtggcata
      661 cttaaacttt ttgccggcaa aatttatttt tagagagaat ccatcccacg caggctcgtt
      721 gtttggagta tagcccacgt tggcaagctg ttccacgtac gtccaatgac cggaactttc
      781 gactaaataa cgataaccgt ggtatgggtt aaaaattaaa tactccgtcc atgaaccgcc
      841 ataattatct ttacgcacaa caatacccac gcatttgaat ttaatgcctt tgagtacccc
      901 ttcttgactc aagggaatcc attgtttgtg tgaaaatgcc ttctcgtatt tctggcgtaa
      961 taaaagaaca tcgtgcgttg gttctaaaat ggaaccgcaa tgaccgcacc gcaccgattg
     1021 cgatttaccc ggcagcgcta aatgaactgg cccgccacaa ttagggcagc gaaaatcttt
     1081 cgccgcaacg gacgcacttt ctttgggggc taaagattcg tcgacgcgtt cgttcatgcg
     1141 gttaaccctt ggatgcctgc gtcttctgcc ttaaagccat aaacactgcg cactctactg
     1201 agccgtaaat cggcaaagcg taaaatttta cctaaaaaga caattggctt ttcttcgcta
     1261 aagtcaatgg tcgcaaattc atcttttgca ttgacgcagt caacgacagg cgcttcatac
     1321 tcaccggtaa atgaaaaagg gagttccccc tcacccgaca cgactttacc cttttggatg
     1381 tctttcacaa agaatttatt atcgtcaatg taaagaggca ttcccgcttc cagcgatgca
     1441 aattgtaaat cgtctcgtaa agacgacttc gtcaactgtc gtgtgaaaaa atacgtaccg
     1501 ttggcttcac ccaaccatgc agggccacct gcatcgtcgg cgatacttaa aaaccattca
     1561 ttccaaaaac ctaaaccaaa attatattga atgcgtccga taattttgaa tgaatgaccg
     1621 tcgatggtac cggtcgttcc caaatccaaa ggagaaccgt cttccacaag agcagcggtt
     1681 ttgcccactt ctttgagtgc atcttcgctt tgcaccgaca tggtgcggca gtatttacaa
     1741 acagcgtaaa gactgtgcgc gttttcaaaa ataagcggag ctccacacga tgggcactgc
     1801 gactttttca tcgaaatcga acatataaat agacagcaga ttgtcacgca aaaaatattt
     1861 acctcctcgg aatctaacca ttttgcaatt taagttttgc ttgaggctct atccacgcgt
     1921 caaaaatcgg ctttcataat cggttttatc ttaaaagaat tcaacttaaa ggattaaatt
     1981 tactctatgt ttcaatcaat tttacgcttt tttcgttcta aaaaatcgct cacagcagac
     2041 gagatactac gctatcccaa accgcgacaa tacacccgcg aacaacacaa cctccgcatt
     2101 aaagacatcg atcccgacgc cgtaaaaacg gtgcaacgca ttcaacaaat gggctgccgt
     2161 gcatacatcg tcggcggctc gatacgcgat ttactcttag gccgcaaacc caaagattac
     2221 gacatcgtca ccgacgcaca cccgcaagaa ttgcgtcgga tgttcgccaa cagccgcatc
     2281 atcggtaagc gttttcggtt ggtacacgtc gttttcaagg gccataaaat cattgaagtt
     2341 tcaacggcac gttctttgcc ttcaaaccgt gctgccgcaa aaaactctga cgaactttat
     2401 ctaaaacggg ataaccaatt tggtactttc aaagacgacg ccgcgcggcg cgatttcacc
     2461 atcaacgcgc ttatttttga cattaaaaac gaagcgatta ttgattatgc aggaggtttt
     2521 gaagacatcc aaaatcgtgt tgtgcgagtc attgggaatc ccgatatttc attccccgag
     2581 gatccggttc ggatgtaccg cgccgtaaaa tttgccgcgc tattaggctt agatttagat
     2641 gcagcaacgt ataaggcaat tcaaaaatat aagaatttac tcaagaaggc gtctaacgcc
     2701 cgtttacacg aagaatataa taaaattttt cgtagcggta aaactgcgct tgtttttgct
     2761 tcgctcaata attccggcct catggaaaca ctcatgccct cgatacagca agcggaggga
     2821 ttacacaacc gtctcaccga cgaagcannn nnnnnnncct ttggggcgtc gtttagcgat
     2881 tgccgataga atgatccaag aacacgaaga agtaaatctc aatatctact acgcgctatt
     2941 ggctgccagc agtctcacca atatcctaca acaaaaccgc cgcgacagcg aaaaacaatt
     3001 agagcacaag gtcaagggac ctctatctat cgttcaaaaa gatatacaac tcacacgccg
     3061 cgagtacgaa actctcctac aaattttttc aacacaaaat tatttccacc gcgacgtgaa
     3121 cgatcgcaaa ggttgggtga aagaatttca acaacgtaaa atcttcccag aatcgttcac
     3181 cgtctataaa attcttgcac gggcagacga cgacgaagcg gcgctccaga aagcgttgtt
     3241 ttgggaaatt ggcctacgcc atcacttacc agaatccatt cgtaaaagcg ccgtcgagcg
     3301 cgctcaaatc gacgagaaag aatacaatgc ctatacaccc accaaagagg acacgcgcga
     3361 aaataaaaat acttcaaacc agcgtacgcc ttcgcctaag gataaaaacc gccgacgacg
     3421 cggaaggcgc ggaggcaatg ggcgtaataa acaaaagatg ccccccgctc tccccccttc
     3481 ccccgttcct taaaaagtgc atctctttgt tttttatatt tttatttctg cactttttat
     3541 ctcttccatt ttttctacac catcgcccga aaagaaaaaa ctagccatca caacacccaa
     3601 ggcgttcatc gattttcaca aaaaagcgat atacactcgc ctcaaaaaat ctattcaaaa
     3661 agctgatgtt gtttttccgc cgcataaaat acgattagca gtttttaaga aagaaaaaaa
     3721 agtagagcta tggatgccca atgtggccaa ccagtggcgt tttgtaaaag aatacgcgtt
     3781 taccgccaac agcggcgaga gtggcccaaa gttatattac ggcgacttac aaatacccga
     3841 aggaatttac ggcatcgact cgatggcgtt gagcactgaa tatcatttag cgctgcacgt
     3901 aaactatccc aacgatttcg acaaggcaat gctcgcttta gaagggcgcg acccccacta
     3961 cgtttctacg ggaatcaata tacatggcgg caacattagc tacggttgtg tcgtcgtcgg
     4021 taacactaac atcgaagaaa ttttttatct cacgtacctt gccggaaaaa ataatacaga
     4081 gctttacatt tttccct
//
LOCUS       DAOMPD010000234         4087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9089_length_4087_cov_2.238839, whole genome shotgun sequence.
ACCESSION   DAOMPD010000234 DAOMPD010000000
VERSION     DAOMPD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9089_length_4087_cov_2.238839"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>940
                     /locus_tag="PLY93_08770"
     CDS             <1..>940
                     /locus_tag="PLY93_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRP69609.1"
                     /translation="IHTNESLTPEVGIIGHSPMGTAIAAALANKQVNIRVYIPSADKA
                     KAFNEMNTDLGNFPLFKLPPNITFTANPKDLFKCGLYVQAVRPWELDEYYEKITPILN
                     ESYATIVGVVKGFTGSKYGIIFEDLEKIFKINPGRFAVMSGANYPEHILERKPCGFEL
                     AAVNTSLLAELIELFTTGYVFTRPAVNPYDVRGLQLGGALKNIYALGIGLVDGYYEKY
                     LGGNNDSTLFHLAHRISAEMANLVVEMGGRKSTLAGVAGITDLMLSCFGQDSHNRQYG
                     HDFVFETHNKSKTLSGVYGIKALPRLVKLRDGKFPIA"
     assembly_gap    941..950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1157..1894
                     /locus_tag="PLY93_08775"
     CDS             1157..1894
                     /locus_tag="PLY93_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69610.1"
                     /translation="MEYVAFLFLGLGVLLVLAGVLLQMREKQGGTSSIRIDDFLRARS
                     KTTKEEDMSKGNDPLPLNDAPVRTPSMPQSPIMSSTPQFPLGNNYAPQSVAQPAFATH
                     AVPGHFQFVENPPPVSTPQVQQEKVAVSIQRNNPRLFEKFAFLYLDSSEKNVYDGASG
                     AAAFDLSEVSGIRRFGRGVFAYDGFSFYFDHTSGQERFPLANLKTVGFYPNCVALTFR
                     DNQPVALLFMEETDSIRKVLETFRTGG"
     gene            1894..>2413
                     /locus_tag="PLY93_08780"
     CDS             1894..>2413
                     /locus_tag="PLY93_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69611.1"
                     /translation="MRAYDFNTHNKTVKRDKSRYFSYAFIFLLVAALAAFIYYVALNS
                     PRIMYYFRENKYSGIEKAHEAALAAIQNEKDADAQTLLQNSEIVDFIDLAHTLQKDHS
                     EDAILVYHEATLLAEVLRKQVYANNQAIIFLFFRDFIQKPIFPPDFDYALWQRVVLLG
                     RKARALGLPDVLA"
     assembly_gap    2414..2423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2424..2927
                     /locus_tag="PLY93_08785"
     CDS             <2424..2927
                     /locus_tag="PLY93_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69612.1"
                     /translation="KELEIDAYLIGGRPWWESAVEFGDIASPIRKTPAWHLIQTGLVR
                     ETPDFELIKTAYGATLATFVKGCYYLRSGNSPLGISNFRDLSKSPIDAGARDAALYVL
                     GYLSGRDKRVKEQLSYYAQIRFPEFAPQNPFFVSEYNYLLRFLGQKSEADQLMRAWDE
                     LRQEQGG"
     gene            3177..>4087
                     /locus_tag="PLY93_08790"
     CDS             3177..>4087
                     /locus_tag="PLY93_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HRP69613.1"
                     /translation="MLSRLKEFFRPNLERKKYKKEFSDELNQQLAGWARVGALIGTVA
                     WLDFAFHTDKQLHPEFPELLYFRLGLSVLASLVLVLSFIPFTKKYNAASLKVMVTYVI
                     LATSWFTGRIANDPNYVSGLQIVVMIPIAAPVAYWQFLSMQIASVIVFFISVFIYKPN
                     LSTAATAYSMNNLIISYAIAIAFGFLLDRIRFDSFLKAKNIQIKNAEIEAQIKRVNDL
                     KNQQDGDYFLTAQLLQPLSLNEVTPSRVYIDFLTEQKKKFQFRKWSSEIGGDISSAHS
                     IQLRGKTYVVFINGDVMGKLIQGAGGA"
BASE COUNT         1079 a          897 c          945 g         1146 t
ORIGIN      
        1 taatccacac caacgaatcg ctcacacccg aggtggggat aatcgggcat agccccatgg
       61 gtactgccat cgccgccgcg ttggcgaata aacaagtgaa catccgcgtt tatataccca
      121 gtgccgacaa agcgaaggcg ttcaacgaaa tgaataccga cttggggaac tttcctcttt
      181 ttaagttacc acccaacatt acttttacgg caaaccccaa agacttgttc aaatgtgggc
      241 tttatgtgca agcggtgcgc ccatgggaac tcgatgagta ttatgaaaaa ataacgccaa
      301 tcttgaacga gagctatgcg acgattgtag gcgttgttaa aggctttacc ggttcaaagt
      361 atggtattat ttttgaagac ttagaaaaga ttttcaaaat taacccaggg cgctttgccg
      421 ttatgtcggg ggctaactat cccgagcata ttttggagcg caaaccctgc ggctttgagc
      481 ttgccgccgt caacacatcg ctccttgcag aactgattga acttttcacc acaggctatg
      541 tatttacccg tcccgccgtc aatccatacg atgtgcgggg tttgcaatta ggcggagcgc
      601 ttaaaaatat ttatgcgttg ggtattggct tggtcgacgg ctattatgaa aaatacttgg
      661 gcggtaacaa tgactcgacg ctttttcatt tagcgcaccg tatctctgca gaaatggcga
      721 atttagttgt ggaaatgggc gggcgcaaat cgacgctcgc cggtgtggcg ggaatcaccg
      781 atctcatgct gagttgtttt ggccaagaca gccacaaccg gcaatacggc cacgatttcg
      841 tctttgaaac gcacaataaa tccaagacgc tttcgggggt ttatggtatt aaagcactgc
      901 ctcgacttgt taaattacgc gatgggaaat ttccgattgc nnnnnnnnnn aaagcaatat
      961 acgatatcat tatcgatggt aaaaaactcg acgatgccat cgaacatatc gcggatacat
     1021 tctccatttc acgcagcgtc caatttcggg attaacggag cttttggggc gacttatggc
     1081 tgtgtcagca ggtttttaag agcctgttta ttaaaacttg ctaaaagaaa cgacataact
     1141 ccacgataat ataaacgtgg aatacgtcgc atttttgttt ttaggtttgg gggtgctctt
     1201 agtgctcgcg ggggtgctct tgcaaatgcg agagaagcag ggaggaacat cgagtatccg
     1261 catcgacgat tttttaagag cgcgcagcaa aactactaaa gaagaggata tgtctaaagg
     1321 gaatgatccg ctccctctca acgacgcacc ggtacgcact ccttctatgc cccaatcccc
     1381 cattatgagt tcaacaccgc aattccctct agggaataac tatgcgccgc aaagcgtggc
     1441 acagccagct tttgccacac acgcggtacc aggtcatttt caatttgttg aaaatcctcc
     1501 accggtgagt acacctcaag tgcagcaaga gaaagtggcg gtgagtatcc aacgcaacaa
     1561 cccgcgcctt tttgaaaaat ttgcctttct ttatttggat tcgagcgaaa agaatgtgta
     1621 cgatggcgcc agcggcgctg cggcattcga cctctccgag gtgagcggta tccgccgttt
     1681 tgggagaggc gtttttgcgt acgatggatt tagtttttat ttcgatcaca ccagcggtca
     1741 ggaacgattc ccgttggcaa acttgaaaac agtgggattc tatcccaatt gtgtggcgtt
     1801 gacgtttcgc gacaatcaac cagttgcttt actttttatg gaagaaaccg atagcatccg
     1861 caaggtttta gaaacctttc ggacaggcgg ttaatgcgcg cgtatgattt taacactcat
     1921 aataaaacgg taaagcggga taaaagtcgt tattttagct acgcctttat ttttctactc
     1981 gtagcggcgc ttgccgcgtt tatctattat gtggcgctca actcaccgcg cattatgtat
     2041 tattttcgcg agaataagta ttccggcatt gaaaaagcgc atgaagcggc gttggcggct
     2101 atccaaaatg agaaagatgc agatgctcaa acacttttac aaaatagtga aattgtagat
     2161 tttatcgatt tagcgcacac gctacaaaaa gatcatagtg aagacgccat tcttgtctac
     2221 cacgaagcga cgcttttggc agaagttttg cgtaagcagg tctatgccaa caaccaagcc
     2281 ataatttttc ttttctttcg cgactttatt caaaagccta tttttccacc ggattttgat
     2341 tatgcactct ggcaacgtgt cgtgcttttg gggcgtaaag cgcgcgcgtt ggggctccct
     2401 gatgttttag cgcnnnnnnn nnnaaagagc tggaaatcga tgcatacctt atcggtggcc
     2461 gtccttggtg ggaaagcgcg gttgaatttg gcgacatcgc ttcgccaatc cgcaaaacac
     2521 ctgcgtggca tttgattcaa actggactcg tccgtgaaac tcccgatttt gaacttatca
     2581 aaactgcgta cggagccacg cttgctacct ttgtaaaagg ctgttattat ctacgttcgg
     2641 ggaactcacc cttggggatt tctaattttc gcgatttatc caaaagcccc attgatgccg
     2701 gtgcgcgtga cgcagcgctt tatgttttag gttacttgag cggcagagat aagcgagtca
     2761 aagaacagct ttcgtattat gcgcaaatcc gctttcccga gtttgcaccc caaaatcctt
     2821 tttttgtgag tgaatacaac tacctattgc gctttttagg gcaaaaaagt gaagcggatc
     2881 aactcatgcg tgcgtgggat gaactgcggc aagaacaggg aggataaaag cgcggcggcg
     2941 actgtaggct ttccctgagc tgccgcgcac tcgaatacgc cataccatta cgcagcggat
     3001 aagtctgttt gaataggcta cggtcggctg cataccacca ttatataatg ccaattattt
     3061 tggttagtat cgaaaatggc ttaggatcgc gacgaagtct ttgtacgtag tttcttaaaa
     3121 ttattacaac ataactttat agatgactta ctattttcct attctaaaat gtcgctatgc
     3181 tttcgcgact caaagaattt tttcgtccaa acctggagcg aaaaaaatat aaaaaggagt
     3241 ttagcgacga actcaaccag caacttgctg gttgggcgcg cgtgggagcg cttattggta
     3301 ccgtcgcgtg gcttgatttt gctttccaca ccgataaaca actacaccct gaatttcccg
     3361 aattacttta ttttcgctta gggctttctg tacttgcatc gctcgttctt gtgttatcgt
     3421 ttattccctt tacaaaaaaa tataacgcag ccagcttaaa agtcatggtt acgtatgtga
     3481 tactggctac ctcgtggttt accggacgga ttgccaacga tcccaattat gtgtcagggt
     3541 tacaaattgt ggtgatgatt cctattgcgg cgccggttgc gtattggcaa tttttatcaa
     3601 tgcaaatagc gtctgtaata gtttttttta tttcagtgtt tatctataaa cctaatttat
     3661 cgactgctgc tacggcgtat tcaatgaaca atcttatcat ttcgtatgcg atcgccatcg
     3721 cgttcggttt tttactcgat agaattcgtt ttgattcatt tctcaaagca aaaaatattc
     3781 aaataaaaaa tgcagaaata gaagcgcaga ttaaacgggt gaacgactta aaaaatcagc
     3841 aagacgggga ttactttttg accgcgcaat tattacagcc gctttcattg aatgaggtga
     3901 cgccttcgcg cgtttatatc gacttcctca cagagcaaaa aaagaagttt caattccgca
     3961 agtggtcttc tgaaattggt ggagatatta gttcggcgca tagcattcag ttgcgtggca
     4021 agacatacgt tgtttttatc aacggcgatg tgatggggaa attgatacaa ggagcgggcg
     4081 gtgcgtt
//
LOCUS       DAOMPD010000235         4078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9118_length_4078_cov_2.299528, whole genome shotgun sequence.
ACCESSION   DAOMPD010000235 DAOMPD010000000
VERSION     DAOMPD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9118_length_4078_cov_2.299528"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..>777)
                     /locus_tag="PLY93_08795"
     CDS             complement(219..>777)
                     /locus_tag="PLY93_08795"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRP69614.1"
                     /translation="PGIKHSAADYYALRLFDFLLGGDSFNSQLTQTIRTEKGWAYTAY
                     STFETDDFTGSVLLFTQTANANLPDVVAAMDAILAHPESFITTDKIERAKISLKNKFV
                     FLFESPAQYLKLYLNLKWDGLPETYLAHYIENLSRVTYADILRVAKKYYQPQNFTLLI
                     CGPKEAYQKKSALRPSEPKILTLEK"
     assembly_gap    778..787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<788..1685)
                     /locus_tag="PLY93_08800"
     CDS             complement(<788..1685)
                     /locus_tag="PLY93_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRP69615.1"
                     /translation="MSINKISPLFFFALVVSYVRVWPAPSSAKPQLDVTTRPWYHYIK
                     EETPETKIPQINHFKLSNGIGVYFYPADAVPLVTAQIFIDGGGFEVADDKLGLQTLWG
                     DTVVFSGSETMGRDTLSSYLEERASKFNFNAELDRSSFNVQSLAHYFEKDLRTLFSVI
                     ENPRFENNDFELLRTRLVKSLEKRDEDPSKWAALGLSQVYWKNTLRGRYATIRTVKNI
                     AREDLTLWQKKLWRAERLTIAVSGSIDEKRLKTLLEETFGKMPYDKTQKADLSRLHVE
                     PTFKPNSLYVLPKEIPQTTVLYK"
     gene            complement(1686..2492)
                     /locus_tag="PLY93_08805"
     CDS             complement(1686..2492)
                     /locus_tag="PLY93_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRP69616.1"
                     /translation="MRIKQFYILLTGTILLTIQPKLHAVDDKPKELLAVALNANGECF
                     YERDGKTQNVRIKTIFFKNDRLFTKNGKMNIQIGPNAVLHLSPYGTVRLNELNESEQK
                     IQIVVSLDQGRGYTKFTKPMKPGSKYQIRTPTITAGVRGTEFIISAGDTPENTHAEDA
                     DIPAGVYVNHGKVAVATVANESAEQEVPAGEQVTGNDELIKGVLDDFVKKKMAMFKRL
                     NTMREEQYRMLENEKRRQMELLEKVRSSRNFENRQKSFEELKKRNNSRFK"
     gene            2694..2870
                     /locus_tag="PLY93_08810"
     CDS             2694..2870
                     /locus_tag="PLY93_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69617.1"
                     /translation="MPNTTHERSEDALADGIADFWQKLDSDASKNLNAEEKKNESLIL
                     EWAGKLSKILQGKK"
     gene            2867..3529
                     /locus_tag="PLY93_08815"
     CDS             2867..3529
                     /locus_tag="PLY93_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRP69618.1"
                     /translation="MKKTERVYLFDIDGTLVNTSGRSAAAFRKALSAVLNFDPQWRGP
                     ELAGQTDASLYARALADTQREDNTTMRTAFFQSYHSHLEENLQTNPPHILTGVHEILS
                     HLKEINATLALLTGNTERGSQLKLNDLFSYFEFGFYGEEHIDRNDLGHAARKRIDAQY
                     GKNFPVTIVGDTPKDIACARAMGADVIAVATGPFSVDELLHADRVLPDLSHWFSANIN
                     II"
     gene            3658..3876
                     /locus_tag="PLY93_08820"
                     /pseudo
     CDS             3658..3876
                     /locus_tag="PLY93_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
BASE COUNT         1037 a          960 c          955 g         1116 t
ORIGIN      
        1 aatatttttt ttctcggtga aattgtaacc cgaaagtgtg cggttttgcg catcggtgac
       61 ccacaggatc gaaccatcgt atgcaagccc taaaggtatt tttgtgtcga gttcaatgtt
      121 gtcgctccaa atgccggatg cgcatagaac caagaacagg gcggcattcg cgattctttt
      181 cgtgttctct atcagtattc gtgaaaaaaa agagcgcatc atttttcaag cgttagtatt
      241 ttgggttccg acggccttaa cgccgatttt ttttggtacg cttccttggg gccgcagatg
      301 agaagagtaa agttttgcgg ttgataatat tttttagcga cacgcaagat atctgcgtac
      361 gtaacgcgcg ataaattttc tatatagtgc gctaaatatg tttcgggaag gccatcccat
      421 ttgagattca aatagagttt gagatactgc gccggacttt caaaaagaaa cacaaattta
      481 tttttgagtg aaatttttgc ccgttcaatt ttgtccgtcg taatgaagct ttcaggatga
      541 gcaaggattg catccattgc ggccacaaca tcgggcaggt tggcgtttgc cgtttgcgtg
      601 aaaagcagca cactgccggt gaagtcgtcg gtctcgaatg tggagtacgc cgtgtacgcc
      661 caaccctttt cagtgcggat cgtttgagtc agttgtgagt tgaaggaatc gccgccgagc
      721 aaaaaatcaa acaggcggag cgcataataa tccgccgccg aatgttttat ccctgggnnn
      781 nnnnnnnctt tatagagcac cgttgtttgc gggatttctt tcgggagcac gtagagcgaa
      841 ttaggtttga atgtcggttc gacgtgcaaa cgcgacaaat ccgccttttg ggtcttatca
      901 taaggcattt tcccaaaagt ttcttctaaa agtgttttta atcttttctc gtcgatggaa
      961 ccagaaacgg caatcgttaa ccgctcggcg cgccatagct ttttttgcca cagcgttaaa
     1021 tcttcccgcg cgatattttt gactgtgcgt atcgttgcat agcggccacg cagggtgttt
     1081 ttccagtaga cttgcgaaag ccccaacgca gcccattttg aagggtcttc gtcgcgcttt
     1141 tctaaacttt tgaccagacg cgtgcgcagt agttcaaagt cgttattttc aaaacgggga
     1201 ttttcaatca ccgaaaaaag cgtgcgtaag tctttttcaa aatagtgcgc taacgattgc
     1261 acgttaaaac tcgaacggtc aagctcggcg ttaaaattaa attttgaagc acgctcttcc
     1321 aaataactcg atagcgtatc gcgccccatc gtttccgagc ccgaaaagac aaccgtgtcg
     1381 ccccaaagcg tctgcaatcc caatttgtcg tcggcgactt caaaaccacc gccgtcgata
     1441 aaaatttgag ctgtcaccaa tggtaccgcg tcggcgggat aaaaatagac gccgatgccg
     1501 ttagagagct tgaaatggtt tatctgtgga atttttgtct caggtgtttc ttctttaatg
     1561 tagtgatacc aaggacgcgt tgtcacatcg agttgtggct ttgccgagga tggcgccggc
     1621 catactctga catacgaaac gacaagcgca aaaaagaaaa ggggacttat tttatttata
     1681 ctcatttatt tgaaccggct gttgttgcgt ttcttgagct cctcgaatga cttttgacga
     1741 ttctcaaaat tacgtgagct gcgtactttt tctaagagtt ccatttggcg tcgtttttca
     1801 ttctcgagca tgcggtattg ttcctcgcgc attgtgttga ggcgtttgaa catcgccatt
     1861 tttttcttca caaaatcgtc gagcactccc ttgatgagct cgtcgttacc cgtcacttgc
     1921 tcgcccgcgg gcacttcttg ctcggcgctt tcgttggcga ccgttgccac cgccacttta
     1981 ccgtggttta cataaacccc cgctggaata tcggcgtctt cggcatgagt gttttcaggg
     2041 gtgtctcctg cgctaatgat aaattcggta ccgcgcaccc ccgctgtaat cgtcggcgta
     2101 cgaatttggt actttgaacc tggtttcatg ggcttggtga atttagtata accacggcct
     2161 tgatcgagcg atacgacgat ttgtattttt tgttcgcttt cattcaattc gttgagacgc
     2221 actgttccat acggcgataa atgcaacacc gcgttggggc caatctggat attcatttta
     2281 ccgttttttg taaaaagtcg atcgttcttg aaaaaaattg tttttatccg cacgttttgt
     2341 gttttgccat cgcgttcata aaaacattcg ccgtttgcgt tcaacgccac agcgagcaat
     2401 tctttgggtt tatcatcgac cgcatggagt ttgggttgta ttgtcagtag gattgtccct
     2461 gttaataaaa tataaaactg ttttatacgc atagttatcc tttttttgtt aacgtggtta
     2521 ggccggaact cgctgagaca aattgttccc atttgctcct tgttttggcc tcgcgagtcg
     2581 gcgcacagtc tcctgaccac taacgtaaca ttatattaga cgtttctcac accgaaaagg
     2641 ttatttttat gccacgtggt tgaccataca ctctctttca agaactcgac atcatgccca
     2701 acaccaccca cgagcggtcc gaagatgcgc tcgctgacgg tatcgcggat ttctggcaaa
     2761 agctcgacag cgacgcgtca aaaaatttga atgcagaaga gaaaaaaaat gaatcgctca
     2821 ttttggaatg ggctggtaaa ctttccaaaa ttctccaagg gaaaaaatga aaaaaaccga
     2881 acgcgtctat cttttcgata tcgatggcac tctcgtcaac accagtgggc gcagcgctgc
     2941 ggcgtttaga aaagcactca gcgcagtgct taactttgac ccccagtggc gtggccccga
     3001 acttgcgggg caaaccgacg catcgcttta cgctcgtgcg ctcgccgata cccaaagaga
     3061 agacaacacc acgatgcgaa cggcattttt tcaaagctac cattcgcatt tggaagaaaa
     3121 tttgcaaaca aaccctccgc acattctaac cggagtgcac gaaatcctgt cgcatcttaa
     3181 agagataaat gccacgctcg cgttgctcac cggcaacaca gaacgcggta gccaacttaa
     3241 actcaacgat ttattttcgt attttgaatt tggtttttat ggcgaagagc acatcgaccg
     3301 caacgacttg ggacacgccg cgcgtaaaag aatcgatgcc caatacggaa aaaatttccc
     3361 cgtaaccatc gtcggcgaca cccccaaaga tatcgcttgt gcgcgggcga tgggggcaga
     3421 cgtgattgcc gtggcgaccg gcccttttag cgttgacgaa ctcttgcacg ccgatcgtgt
     3481 gctgcccgat ttatcgcact ggtttagcgc taacataaac ataatttgat tgattccttg
     3541 tgcgttgacg cgcagattca aaaccggcaa gcgcgctggg gtggcatgaa caaactcacc
     3601 gacgagcata tccagaagat tgttgaaagg taccaacatt gacgcgaaga accgcgctat
     3661 tcccgtcggg taatgatgga agagattgcc aacaacgatt acaacctcaa tatttcacgt
     3721 tacatcagta cggcaaaggc agaagaagaa attgatctga aagcggtgaa tgctcggtta
     3781 gtcgagttcg actcaaagat taccgaggcg acgaagaaac ataacgcatt tctgaaagag
     3841 ctggggttac cactgctgcc aacagtaaat cgatgaaatg tatctcattg acaaaactga
     3901 taagacgcga tgccgcgccg gcatggacgc cggcgaataa caagggcgca tctatgtcac
     3961 acaacgctgg atttacaaga cgtaaagtga cgcttgtgtc aactttgtgt ctctaaagcc
     4021 gaattgcgtc actttatgtg ccgagggcac ctgccgacga gcgcaaaatg cacttctg
//
LOCUS       DAOMPD010000236         4050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9193_length_4050_cov_2.606008, whole genome shotgun sequence.
ACCESSION   DAOMPD010000236 DAOMPD010000000
VERSION     DAOMPD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9193_length_4050_cov_2.606008"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLY93_08825"
     CDS             <1..336
                     /locus_tag="PLY93_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69619.1"
                     /translation="ANARAGFTFLSIYENLTIPPDKIINAITTLKTPLHQNVLHALTS
                     AAAATSFVQFYNDATKEEKTKFRISTFGLSATEILVDKGFNPYHRSDAPTFDSYIDEL
                     LGKTTLMQI"
     gene            347..1696
                     /locus_tag="PLY93_08830"
     CDS             347..1696
                     /locus_tag="PLY93_08830"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRP69620.1"
                     /translation="MEHRKSQKLWKRALEVFPGGVNSPVRAFRSVGGEPVFIESGRGA
                     YVYDTDGNKYLDFLSSWGPLILGHAPRKVTKAIRRAAVEGSTFGAVTEREVLLGEFIL
                     SQLRFVDKIRFVNSGTEAVMTALRLARGVTGRKMIIKFEGCYHGHSDSLLAKAGSGLL
                     TLAGNIAEASSPGVPDEIVKNTLVLPLNDKAHFEDALKKHAMHTAAVIIEPVPANAGL
                     LLQTRDFINYIVEASKAKGILVIFDEVISGFRLGFTGYAGREKLTPDIVTYGKIIGGG
                     LPVGALAGKKQIMDQLSPQGAVYQAGTLSGNPLAMAAGLAALKQLTHENGAVYRKLKE
                     LSAYLQENFTEEIAPLFQERDWQIQLIQDESLFWFSFQKKGAPVIVRSVQDIEPFSAK
                     IYAKIFHALLGKGIYIAPSAYEVGFLNNAMRRRDIDFFIKTLKIVIQSLPKEVQHFS"
     gene            1693..2760
                     /locus_tag="PLY93_08835"
     CDS             1693..2760
                     /locus_tag="PLY93_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRP69621.1"
                     /translation="MSDVQRKNLSRISLFSGSTLLKAFSMLSVIGLLFWWQHLIERNL
                     ENQYNFFAKEISAGTVSNALRELIEKQDRTLAAQIFSTPKSNSVNSTSIQIIHARLLN
                     RQNMLFYEQSFFILLLLSGHFFFVYIYFREHRKRKQIEQTILLATHELRQPLQSLSLA
                     LEAVHPKATEKTQAALELGLNDIHRLGELVRYLAGAFATKKDTSFFLITNLETFIENF
                     IETSFSSVKKRIRTDTKASPSFRLNIAAERLHFLFRNLTENALKYAKDTIQIHAKLEE
                     KYFIFSVMNSGASIDSNSFKKIGSAFYRATSVEVQNKTGFGLGLYLSGLIARQAGGTL
                     TIENTKEGITRTILKLRRYRA"
     gene            2757..3449
                     /locus_tag="PLY93_08840"
     CDS             2757..3449
                     /locus_tag="PLY93_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP69622.1"
                     /translation="MSREKHIAIVEDDDSIRELLAEHLKEAGYRVSAFFSAEQLERKL
                     DAQYNLTLLDIMLPGKSGLQLLKDIRAKGVDFPVILITASESDVHIDAALSDGANDYI
                     VKPFNLKHLLLKIENLFHQIDAKNQSESNDVYTLGAGKFEVGLQTVLRNKKEYKLTPS
                     EAATLMYFIENSNRIISRDELQEHIGAHAKAISSRNLDNYILKFRKLFEFDPKDPVLF
                     VTIPRKGYALKR"
     gene            3487..>4050
                     /locus_tag="PLY93_08845"
     CDS             3487..>4050
                     /locus_tag="PLY93_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRP69623.1"
                     /translation="MTNTQNNFDLAARGLKTECTPIWFMRQAGRYLPEYREIRKKNSF
                     DAMLANADVATEVTLQPIRRFDLDAAVIFADIMTILSALDIPFDFSSGGPALQFNIDD
                     AIDVEKLSAERVIDNQKKLNAFLTTIRQTRAALKADKAVVGFAAAPFTLLSYLIEGKT
                     SKTHNRTRAFMAAYPERFSSLMRKITAC"
BASE COUNT         1151 a          965 c          889 g         1045 t
ORIGIN      
        1 gcaaacgcac gcgccggttt tactttcctt tcaatttacg aaaacttaac aatcccgccc
       61 gataaaatta ttaacgcaat cactacatta aaaacgccgc tacaccaaaa tgttttacac
      121 gcattgacat ccgccgccgc cgccaccagt tttgttcagt tctacaatga cgcaactaaa
      181 gaagagaaaa caaaatttcg tatttcaact tttggtttgt cggcaacgga gattctcgtc
      241 gacaaggggt tcaatccata ccaccgcagt gacgctccca cttttgattc ttatatcgat
      301 gaacttttag gtaaaacgac gctcatgcaa atttaaggaa ataattatgg aacacaggaa
      361 atcacaaaaa ctctggaagc gcgcactcga agtttttccg gggggagtca attcaccggt
      421 gcgtgcgttc cggtcggtgg gcggcgaacc cgtctttatc gaatcgggtc gtggcgcgta
      481 cgtgtacgat accgacggga ataaatattt agactttttg tcgagttggg gaccgctcat
      541 tttaggccat gcgcctcgga aggtaacgaa agcaatccgc agagccgccg tcgaaggttc
      601 gacctttggc gcagtcaccg aacgcgaagt ccttttgggt gaatttattt tatcgcagtt
      661 gcgctttgtc gataaaattc gttttgtcaa ctcgggtacc gaagcagtga tgacggcgct
      721 acgcttagcg cgcggcgtca ccggacgcaa gatgattatc aaatttgaag gctgttacca
      781 cggccacagc gactcgcttc ttgcaaaagc gggttcggga cttttaactc tcgcggggaa
      841 tatcgccgag gccagctctc ccggggtacc cgatgaaatt gtgaaaaaca cattggtgtt
      901 accgttaaac gacaaagctc attttgaaga cgcgctcaaa aaacacgcca tgcataccgc
      961 tgccgtcatc atcgagcctg tgccggcaaa cgcagggctt cttttgcaaa cacgagattt
     1021 tatcaactac atcgtggaag cgtctaaagc gaaaggaata cttgttattt ttgatgaagt
     1081 gatcagcggt tttagattgg gtttcaccgg ttatgcagga cgcgaaaaac tgacacccga
     1141 tattgtcacg tacggtaaaa ttatcggcgg tggattaccc gttggcgcgc ttgcgggtaa
     1201 aaaacaaatt atggatcaac tctcgccgca aggggcagtc taccaagcgg gtactctctc
     1261 gggaaatcct ctcgcgatgg ccgccgggct tgctgcgcta aaacaactga cgcacgaaaa
     1321 cggcgcggta taccgtaagc tcaaagaact ctccgcgtac ttgcaagaaa atttcacaga
     1381 agaaatcgct cctctttttc aagagcgcga ctggcaaatc caattaatac aagatgaatc
     1441 gcttttctgg ttttcgttcc agaaaaaagg cgcgccagtc atcgtccgca gcgttcaaga
     1501 catagaaccc ttctctgcaa aaatttacgc caaaattttt cacgcgctct tgggaaaagg
     1561 aatctatatc gcgccgtctg cgtacgaagt gggttttttg aataacgcga tgcgccgccg
     1621 tgacattgat ttttttatca aaacgctaaa aattgtgatt caatcattgc caaaagaggt
     1681 ccagcatttt tcatgagtga cgttcagcga aaaaatttgt cgcgtatttc tcttttttcc
     1741 ggttcgactt tgctgaaggc attttcgatg ctgtcggtga ttggtttact cttttggtgg
     1801 cagcacctta tcgagcgcaa tttagaaaac cagtacaatt tttttgccaa agaaatttcg
     1861 gcgggaacgg tatctaacgc gctgcgcgaa cttatcgaaa agcaagaccg cactcttgcg
     1921 gcacaaattt tcagtacgcc gaaaagtaac agcgtgaatt ctacttcgat acaaattatt
     1981 cacgcgcggt tactcaaccg acaaaacatg cttttttatg agcagtcttt ttttattctt
     2041 cttttactct cggggcactt tttttttgta tatatttatt tccgcgaaca ccgcaagcgc
     2101 aaacaaatcg aacaaacgat cttgctcgct acgcacgaat tgcgacaacc gttgcaatcg
     2161 ctttctttag cgttagaggc tgtccaccct aaagcgacgg aaaagacgca agcagcgctg
     2221 gagttgggat taaacgacat ccatcgcttg ggcgaactgg tgcgctactt agcaggcgca
     2281 ttcgccacaa aaaaggatac aagttttttt ctcattacaa accttgaaac gtttattgaa
     2341 aattttattg agaccagttt ttcaagcgtt aaaaaaagaa tacgcaccga cacgaaagcc
     2401 tcgccttctt tccgtttaaa catcgctgcg gagagactgc attttttgtt ccgcaatctc
     2461 accgaaaacg cgctgaaata cgcgaaagac accatacaaa tccatgcgaa gcttgaagag
     2521 aagtatttta ttttttccgt tatgaattca ggtgcgtcga ttgattccaa ctcgtttaaa
     2581 aaaattggga gcgcgtttta ccgcgccacc agcgtcgaag tgcaaaacaa aacagggttt
     2641 ggccttggcc tttacctctc ggggttaatc gctcgacaag caggcggtac actgactatc
     2701 gaaaacacca aagagggcat cacacgaacc attcttaaat taaggagata ccgcgcatga
     2761 gccgtgaaaa acatatcgcc atcgttgaag acgacgactc aattcgagaa ctactcgccg
     2821 aacatttaaa agaagcgggc tatcgagtgt cggctttttt tagcgcagaa caactggaac
     2881 gcaaattaga cgcgcaatat aatttaacgc tcttggatat tatgttgccc ggaaaaagtg
     2941 gattacaact tttgaaagac attcgtgcca aaggggtcga ttttcctgtt attctaatca
     3001 ccgcttctga aagcgatgtg catattgacg ccgcactttc cgatggcgcg aacgactata
     3061 tcgtaaagcc ttttaacctc aagcatttgc ttttaaaaat tgaaaattta ttccatcaaa
     3121 tcgacgcgaa aaaccaaagt gaatccaacg atgtttatac tttaggcgca gggaaatttg
     3181 aagtggggct acaaaccgtg ctgcgtaaca aaaaggaata taaactcacg ccatcggagg
     3241 cggcaacgtt gatgtatttt attgaaaatt caaaccgcat cattagccgc gacgaactac
     3301 aagaacacat cggcgcgcat gcgaaagcaa tttcatcgcg caatctcgat aactatattt
     3361 tgaaattcag aaagcttttt gaattcgatc ctaaagaccc tgtactcttt gtgacgatcc
     3421 cccgtaaggg gtatgcgcta aagcggtaat ccataaataa aatagaagga acagtattta
     3481 atttttatga caaacacgca aaacaacttt gacttggccg cacgtggctt aaagaccgag
     3541 tgcacaccta tttggtttat gcgtcaagca gggcgctatt tacccgaata ccgcgagata
     3601 cgtaaaaaaa attcattcga tgcaatgctc gcaaacgccg atgtcgccac cgaggtgact
     3661 ctgcaaccca tccggcgatt tgatctcgac gccgccgtaa ttttcgccga catcatgacc
     3721 attttatcgg cgctcgacat tcctttcgat ttttcatcgg gtggccccgc gcttcaattc
     3781 aatatcgacg atgcaatcga cgttgaaaaa ctgagcgcag agcgggtcat cgataaccaa
     3841 aaaaagctca atgcgttttt aacgacaatt cgtcaaacgc gcgccgcact taaagcagat
     3901 aaagcggtcg ttggttttgc cgcagcgcca tttactcttt taagttacct catcgaaggc
     3961 aaaacctcga aaacgcataa ccgcacccgt gcttttatgg cggcgtatcc cgagcgtttc
     4021 tcaagcctta tgcgcaagat tacggcatgc
//
LOCUS       DAOMPD010000237         4039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9227_length_4039_cov_2.321285, whole genome shotgun sequence.
ACCESSION   DAOMPD010000237 DAOMPD010000000
VERSION     DAOMPD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9227_length_4039_cov_2.321285"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLY93_08850"
     CDS             <1..504
                     /locus_tag="PLY93_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain protein"
                     /protein_id="HRP69624.1"
                     /translation="GAAAVLLSNNAYALHLPRTHLGVRGSEKAILAEGKVEVDGHIVV
                     KNAGSISVALPKASNEAFVDGDLITIDNGVASKMRQENQPLVGVVSKEAALQLESGQG
                     ALKVAVAGVVSLRVHGQVKAGDKLALNTAQAGTCKVGQGQEKFFAVALEASTNDREKS
                     VLAILVR"
     gene            501..1145
                     /gene="mtnB"
                     /locus_tag="PLY93_08855"
     CDS             501..1145
                     /gene="mtnB"
                     /locus_tag="PLY93_08855"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HRP69625.1"
                     /translation="MTEDNLKDHPLAHELAGCGNLFHRWQWSLASSSNYSALLDAEQI
                     IISQSGVDKERMTSADFMLVDFDAKPLAGFEVLKPSAETALHCQIYQKADLFHAGSVL
                     HTHSKFSVFFSRRHLAEGKIIFSGFEMQKIFDGIATHESEIEIAVFKNSQTMRDITKA
                     FDDYLTTNKIPAAYLIEGHGVYTWAKTILHAKQKLEALEHLFELKYMEELCKRH"
     gene            1130..1672
                     /locus_tag="PLY93_08860"
     CDS             1130..1672
                     /locus_tag="PLY93_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HRP69626.1"
                     /translation="MQTTLTIYSEKNPNEKEEVIQEFGKIKDLLATQRIGFERWQAEK
                     PLSEKATPEEVADAYRTSIDKILSQGYKTFDVVSLYPDAPNAAEARKKFLSEHTHGED
                     EVRFFVDGSGMFYVHADGKVFMLLCTKGDFISLPAGTTHWFDMGPKPFFKAIRVFNNP
                     EGWVGHFTGSRIADQFPKYE"
     gene            1676..2377
                     /gene="mtnC"
                     /locus_tag="PLY93_08865"
     CDS             1676..2377
                     /gene="mtnC"
                     /locus_tag="PLY93_08865"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HRP69627.1"
                     /translation="MADIRVVLCDIEGTTSSISFVHKVLFPLSLKKMEGYLSANSIKE
                     DIKSHLDVLWKKIQPADANHREKILLQTLEGYIKSDVKDTTLKYIQGKIWHEAFLAGE
                     VKGHVYPEVASFFQKWSSHSKKIFIYSSGSIQAQKDLFQHSQAGDLSVYLSGYFDTTT
                     GMKRDALSYRKIQNAIGVDAAAILFLSDVVEELNAAREADMQTCLLLREGASAPESYD
                     GAKALDFAVVDSLFF"
     gene            2411..3895
                     /gene="glpK"
                     /locus_tag="PLY93_08870"
     CDS             2411..3895
                     /gene="glpK"
                     /locus_tag="PLY93_08870"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRP69628.1"
                     /translation="MAKPFIITIDQGTTGSRVFLISERGEVLESAYEEFTQYFPRAGW
                     VEHNANEIWSSVSRLLAKVLENSGLNATDAVAIGITNQRETTVVWEKKNGAPIHNAIV
                     WQCRRTADYCEELKKEGLGNLFRDKTGLVLDAYFSGTKIKYILDTVPQAREKASRGDL
                     LFGTIDSFLLYKLTGEHKTDFTNASRTLIFNIHERKWDSELCKILNIPENILPEAQPS
                     KSLFGKTKGLQVLPDGIPVYSMIGDQQSALYGQLCHNPGEAKNTYGTGCFVVMNLGDK
                     FLQSKNGLITTLACDTEGNPVYALEGSIFIGGAVVQWLRDKMQFFDDSKKSEMLAKSV
                     AMESDVVFVPAFAGLGAPYWDQDARGAIMGLTRDTTAEQITQAALKAIALQSYDVISA
                     MQADSGLPLSTLKVDGGATSNRYLMEFQAGILGAQVVVPRNAETTVLGAAYLAGLGAK
                     LWTSSDDLRKLNPAAATYHAVMSAEQRERELARWKKAVARVLTQ"
BASE COUNT         1141 a          829 c         1017 g         1052 t
ORIGIN      
        1 ggcgcagcgg cggtacttct ctccaacaat gcttatgcgc tgcatttgcc gcgtacgcat
       61 ctaggggtta gaggttcgga aaaagcgatt ctggccgaag gtaaagtcga ggtggatgga
      121 cacattgtcg taaaaaatgc tgggagcata tctgtcgctc tccctaaagc atccaacgaa
      181 gcgtttgtgg acggcgattt aattaccatc gacaacggtg ttgcctcgaa aatgcgacaa
      241 gagaatcaac cgctcgttgg tgttgtgtct aaagaagcgg cgcttcagtt agagagcggg
      301 cagggcgcgc ttaaagttgc agttgctggt gttgtttctt tgcgtgtcca cgggcaagtg
      361 aaagcgggag acaagcttgc actaaacaca gcgcaagcag gcacatgtaa agtggggcag
      421 gggcaagaaa aattttttgc cgttgcattg gaagcgtcga ccaacgatcg ggaaaagtcc
      481 gttttggcga ttttagtccg gtgacagaag acaatcttaa agaccatccg ttggcgcacg
      541 agttagcggg gtgtgggaac ttgtttcacc gttggcaatg gtcgctcgcc agcagcagca
      601 attactcggc tcttttggat gcagagcaaa taattatttc acaatcgggc gtggataaag
      661 aacgcatgac atctgccgat tttatgttgg tggatttcga tgcaaagccg ttggcaggtt
      721 ttgaagtatt gaagccgtcg gcggaaactg cgctgcactg ccagatatac caaaaagcag
      781 atttattcca cgcgggaagt gtattgcaca ctcactcaaa attttctgtc tttttttcaa
      841 gacgacattt agcagaagga aaaattattt tttccggctt tgaaatgcag aaaatttttg
      901 acggtatcgc cacgcatgaa agcgaaattg aaattgctgt tttcaagaat agccaaacaa
      961 tgcgcgatat aaccaaagca tttgacgatt atttaacaac aaataaaata ccggcggcat
     1021 acctcatcga agggcacggt gtttacactt gggcaaaaac cattttacac gctaaacaaa
     1081 aattagaagc tctcgaacat ttgtttgaat tgaagtatat ggaggaatta tgcaaacgac
     1141 attaacgatt tattcagaaa aaaaccccaa cgaaaaagaa gaagttattc aagaatttgg
     1201 aaaaatcaaa gatctattag ccacacaaag aattggtttt gagcgatggc aagcagaaaa
     1261 accactttca gaaaaagcaa ctccagaaga ggtggcggat gcataccgca cttccatcga
     1321 taaaatttta agccaagggt ataaaacatt cgatgttgtt tctttatatc ccgatgcgcc
     1381 caatgcagca gaagcgcgta aaaagttttt gagcgaacac acgcacggtg aagacgaggt
     1441 gcgctttttt gtcgatggct cgggaatgtt ttatgtgcac gccgacggta aagtttttat
     1501 gttgctttgc acgaaaggcg atttcatcag cttgcctgcg gggacaacgc attggtttga
     1561 catgggaccg aaaccttttt tcaaagcgat tcgtgttttc aacaaccccg aaggttgggt
     1621 ggggcatttt actggcagca ggattgccga ccaatttccc aaatacgaat agtcgatggc
     1681 agacattcgt gtggtacttt gcgatattga aggcaccacg tcttccatat cgtttgtgca
     1741 caaagtttta tttcctctgt ctcttaaaaa gatggagggt tatctcagtg caaacagtat
     1801 caaagaagat ataaaatctc atctggatgt actgtggaaa aaaattcaac ccgctgatgc
     1861 aaatcatcga gaaaaaattt tgctgcaaac attggaaggg tatataaaaa gcgatgtcaa
     1921 agacacgacc ttaaaataca ttcaaggaaa aatttggcat gaggcatttc ttgccggtga
     1981 agttaaagga cacgtgtatc cagaggtcgc ttcttttttt caaaagtggt catctcactc
     2041 gaaaaaaatt tttatttaca gctcaggatc catccaagcc caaaaggatt tatttcagca
     2101 ttcgcaagcg ggtgatttga gtgtttattt aagcggctat ttcgacacca caactggtat
     2161 gaagcgtgac gcgctgtcgt atcgaaaaat ccagaatgcg attggtgtcg atgcggcggc
     2221 gattttattt ctttcggatg tcgtcgaaga attaaacgcg gcgcgtgagg cggatatgca
     2281 aacgtgtttg ctgttgcgcg aaggcgcatc tgcgccggag agttatgacg gcgcaaaagc
     2341 acttgatttt gctgtagtgg attctctctt tttttaattg ccttcctctt tcggtaaaga
     2401 aaatacactc atggcaaaac cgtttattat cactatcgat caaggcacta ccggctcgcg
     2461 cgttttttta atttccgagc gcggcgaagt tcttgaaagc gcgtacgagg aattcacgca
     2521 gtattttccc cgcgcgggtt gggtggaaca caacgcgaac gaaatttggt cgagcgtttc
     2581 gcgtcttttg gcaaaggtgc tggagaattc tggcttaaac gcgacagacg ccgttgcgat
     2641 tgggattacc aatcagcgtg aaacaacggt tgtgtgggaa aagaaaaacg gcgcaccgat
     2701 tcacaacgcg attgtgtggc aatgccgccg cacagccgac tattgtgagg agttaaaaaa
     2761 agaaggactc ggcaatctct tccgtgataa aacgggtctt gtgcttgatg cgtatttttc
     2821 gggaacaaaa attaaatata tcttagacac cgtgccgcaa gcgcgcgaaa aagccagtag
     2881 gggagattta ctctttggaa ccattgactc ttttcttctt tataaattga caggggaaca
     2941 taaaactgat ttcaccaacg cttcacgcac gctcattttc aatatccatg agcgcaagtg
     3001 ggatagcgag ctatgtaaaa ttttaaacat tcctgaaaac atcttacccg aagcgcagcc
     3061 atcgaaaagt cttttcggaa aaacgaaagg tctccaagtt ttgcccgatg gcatccctgt
     3121 gtattcgatg attggcgatc agcaatcggc gctttatggt caactttgtc acaatccagg
     3181 agaagcgaaa aatacatatg gtacgggttg ttttgttgtc atgaatttgg gggataagtt
     3241 tttacagagt aaaaacggat tgattacgac gttggcatgc gacaccgaag gaaatccggt
     3301 gtacgcttta gaaggttcta tttttattgg tggtgcagtc gtgcaatggt tgcgcgacaa
     3361 aatgcaattt ttcgatgatt caaaaaagtc ggagatgctt gccaagtccg ttgcaatgga
     3421 atcggatgta gtgtttgtgc cggcattcgc tggacttggt gcgccgtatt gggatcaaga
     3481 cgcgcgtggt gcaattatgg gacttacccg cgatacaacc gccgagcaaa tcacacaggc
     3541 tgcgctcaaa gcgattgcgt tgcaatcgta cgatgtcatt tctgcaatgc aagccgacag
     3601 tggtttaccg ttgtccacat tgaaagtcga cggcggcgca acgagcaacc gctatcttat
     3661 ggagttccaa gcggggatct tgggcgcgca agtggttgtg cctcgaaacg cagaaacgac
     3721 agtgttgggt gcagcgtatc tagcagggtt gggagcgaaa ctgtggacat cgtccgatga
     3781 cttgagaaaa ttaaatccag cggcggcgac ataccacgcg gtgatgagtg cagaacaacg
     3841 cgagcgcgaa cttgcgcgtt ggaaaaaggc cgttgcgcgc gtgctgacgc agtagttatc
     3901 gaattgcttc gagaatatct aacgcgtggg ttttcaccga tactttatca aaaacgtgtg
     3961 taatttttcc attggtgtcg ataacaaacg ttgagcgtaa aattcccatg tacttacgac
     4021 cgtacatcga cttctccgc
//
LOCUS       DAOMPD010000238         4034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9239_length_4034_cov_3.126665, whole genome shotgun sequence.
ACCESSION   DAOMPD010000238 DAOMPD010000000
VERSION     DAOMPD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9239_length_4034_cov_3.126665"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PLY93_08875"
     CDS             complement(<1..1303)
                     /locus_tag="PLY93_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP69629.1"
                     /translation="MENKKNIWATLVEDDALWPQSMAEEERTQLLNKIQDANIAFETQ
                     SDAQSTTFLGRTYHQKQYLLPQRHALWLDKLLRKRGGKTGKNQDDAQTLLKEGNHLLL
                     TGGPGTGKSYTISQFIASLKIKDGRYTRVAICAPTGKAAARFIALEKSSTALVTTATI
                     HRLLGVSEHRNYVQYNSRNPLPYDIVIVDEVSMLNLFLLSSLVHALPPHAQLALVGDL
                     QQLPTIDGIAVDKAIHFLVQEKIVHHIHLTEAKRFSENKNNAYKRIAQEGFAALQDKE
                     VASEIPTICLNDRNTLYREIDAYIEKNLLDKTAQELRGAFNNAAKDEKTSDALLQKAF
                     NFLSQNVLLCERREGAWGSQAFNAYIRDFLEQKKLNRTLEPIINIQNNYTLQLLNGDL
                     GFLYEKDGIVNAYFKQSDGTFRCFRLDELRHVESAYALTIHK"
     gene            complement(1293..>4034)
                     /locus_tag="PLY93_08880"
     CDS             complement(1293..>4034)
                     /locus_tag="PLY93_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRP69630.1"
                     /translation="IIEQEKAKLAEILCVTFTEKAAAELKSRIYQRLKESTSPHAQAA
                     LQEFSQNAIGTIHSFCLLALRDLPNNFLESNDKNADDTILAQDALEEVIRLDWTKLPT
                     ADLANFLLQVEYGKGGLNSDFEKEITAKSAHLFASPSTKIFPDIKEIQQVQTEDDFVA
                     WTIYCTFERMRKACEQKNLLTFSTMITKTADALDDKEFVKKIQKKFRYALIDEFQDTD
                     TIQWKIFSNLFLNKTHKLIVVGDPKQAIYKFRGADVFVYLNAKQEMHHYGALEDQLPQ
                     NFRSTDKILTVLDSIFTNTLVKDVWEKAGFTYTPPTIVRTSNIENPMKFYLYEKEGKK
                     SFPTQGFAYLTAKRIQELKNESPNWSVGVIAFKHETLKVFAKVFREAGIEFAYYNAKP
                     NFSQIEFKHLKIFLQSFALPLEEGFRIAADTLFLRARSIIKNEDTNTIYKYYSYLYQL
                     ANEKKIMSFLQRLAENQAVLHLLLKHDGDALTYHTWRVLIERLLNAVGDSIHDIPSLK
                     NYLLQIESEDTERNGDMLRSPNAATLLTVQSAKGLAWDIVVLADGHNNKQWRNFPFFH
                     NKKGEPVIPANNDSFNANPNKLLSIEDEAKITQLNNLYVALTRAKNYFIGYIAPAHSD
                     KLKGPIADFLYPLRDTIQTLSFNDALENLPPLTLSKQKVLDEKIDHKDIDKRISKQES
                     FSSLVHSSFSEKNFIEDVLPRGTRTGDILHKILEHTDFTTFVDMPSDSKEESIVKYQT
                     QLSYAYPHGEEKTRRYAERVFEIIHACAHTQLTLNEDAVVTLAKLPRSNLNRETPFSS
                     SARTHQILRERKQTPLRTSMHGVMDLFFTNDEKNYYILDYKSNSVSDIAPEYLADYIH
                     ERYHKQAEIYIEALDAYLSKNYPNEDRRVVGCFFMALRYLKAESASGLVFLRTNHGK"
BASE COUNT         1015 a          767 c          945 g         1307 t
ORIGIN      
        1 ttttgtgtat tgtgagtgcg tacgcgcttt ccacatgcct caattcatcg agtctaaaac
       61 aacggaaagt accgtcgctt tgcttaaaat aagcgttcac gataccatct ttttcgtaaa
      121 ggaatcctaa atctccattg aggagttgga gagtgtaatt attttggata ttgatgatgg
      181 gctccaacgt gcggtttaat tttttttgct ctaagaaatc gcggatgtac gcattaaaag
      241 cttggctgcc ccacgcgcct tcgcgccgtt cgcacaagag tacattttgt gataaaaaat
      301 taaacgcttt ttggaggagt gcgtcggatg tcttttcgtc tttagcggca ttattgaatg
      361 caccgcgaag ctcttgggct gtcttgtcga gtaaattttt ttcaatgtaa gcgtcgattt
      421 ccctatagag agtatttcga tcgtttaaac aaatggtagg gatttcactg gcaacttctt
      481 tgtcttgaag agcggcaaaa ccctcttgcg cgatacgttt atacgcatta tttttatttt
      541 ccgagaagcg tttagcttca gttaaatgga tgtgatggac aattttttct tgaaccagaa
      601 agtggattgc tttatcgaca gcaataccgt caatcgtggg caactgttgc aagtcaccca
      661 ccagcgctaa ttgcgcgtgc ggaggaagag catgcacgag agagcttaag agaaaaaggt
      721 tgagcatcga aacttcgtcc acaatgacga tgtcgtaggg gagcggattt cgagagttat
      781 attgaacgta attcctgtgt tcgctcaccc ccaaaaggcg atggatggtg gcggtggtga
      841 cgagcgctgt ggaagatttt tctaaagcga taaatcgtgc agcggcttta ccggtgggcg
      901 cgcaaatggc aacgcgtgtg taccgaccgt ccttaatttt taatgatgcg ataaattgtg
      961 aaatagtgta actcttgcct gtacccgggc caccggttaa taagagatga tttccttctt
     1021 taagaagcgt ttgagcatcg tcctggtttt ttccagtttt gcctccacgc tttctcaaca
     1081 gcttatccag ccaaagcgca tggcgttgcg gtaagaggta ttgcttctgg tggtatgtcc
     1141 gtccaaggaa ggtcgtgctt tgtgcgtcgc tttgggtttc aaaagcaata tttgcgtctt
     1201 gtattttatt taagagttga gtgcgctctt cttcggccat cgattgcggc caaagcgcgt
     1261 cgtcttccac caaggtagcc caaatatttt ttttattttc catggtttgt ccttaaaaac
     1321 actaagccgg atgcgctttc ggctttaagg taacgcaatg ccataaagaa acagccgaca
     1381 acacgacggt cttcattggg ataattttta gatagataag catccaatgc ttctatataa
     1441 atttctgctt gcttgtggta gcgctcgtgg atgtaatcgg ctaagtattc gggggctata
     1501 tcgctcaccg aattactttt gtaatcgaga atgtaataat ttttttcatc gttcgtgaaa
     1561 aataaatcca tgactccgtg catcgaagtg cgtagcggtg tttgcttgcg ttcgcgtaaa
     1621 atttgatgtg ttcgagcaga gctgctaaat ggagtctctc gatttaaatt tgagcgcgga
     1681 agtttggcta aggtaacgac cgcatcctca tttaaagtta attgtgtatg tgcgcaggcg
     1741 tggataatct cgaaaacccg ctctgcgtaa cggcgtgttt tttcctcgcc gtgcgggtaa
     1801 gcgtagctga gttgagtttg gtatttgacg atagattctt cttttgagtc ggatggcata
     1861 tccacaaaag tagtgaagtc agtgtgttcc aatattttat ggagaatatc accggtgcgt
     1921 gtgcctcgtg gcaaaacatc ttcaataaaa tttttttcag aaaaagacga gtggacaagg
     1981 ctgctgaatg actcctgttt tgagatacgc ttatcgatat ctttgtgatc gattttttca
     2041 tcgagaactt tttgtttcga taacgtcaat ggaggtagat tttccagcgc atcgttgaaa
     2101 gatagtgttt gaatggtgtc gcgtaaagga tagagaaaat cggcaatggg acctttaagc
     2161 ttatcgctgt gcgcaggcgc aatataacca ataaaataat tttttgcgcg cgtgagcgca
     2221 acgtataaat tgttgagctg ggtaatcttg gcttcatcct cgatggagag gagtttattc
     2281 ggatttgcat tgaagctgtc attgttcgca ggtattacag gttctccttt tttattatgg
     2341 aaaaatggaa agtttctcca ttgtttgttg ttgtgtccat ctgccaagac gacgatatcc
     2401 catgcaagac ctttagcgct ttgcactgtc aaaagcgttg ctgcgttggg ggagcgtaac
     2461 atatctccgt ttctttctgt gtcttcgctt tcaatttgca gcaaataatt ttttagcgat
     2521 ggaatatcgt gaatactatc tcctaccgca ttgagtaagc gctcaataag aacgcgccac
     2581 gtgtggtatg tcagtgcatc gccatcgtgt tttagaagaa gatggagtac ggcttggttt
     2641 tctgcaaggc gttgcaaaaa actcataatt tttttttcgt ttgccagttg gtagagatag
     2701 gaataatact tatatattgt attggtgtct tcgtttttga tgatacttct tgcgcgtaag
     2761 aaaagcgtgt cggccgctat gcgaaaccct tcctcgaggg gtaacgcgaa cgattgtaaa
     2821 aaaattttaa gatgtttgaa ttcaatttga gaaaaattag gttttgcatt gtagtatgca
     2881 aactcaatcc ccgcttcgcg aaaaactttt gcaaagactt tcagagtttc gtgcttaaaa
     2941 gcgatgacgc ccaccgacca gttgggactt tcattcttta attcttggat gcgttttgct
     3001 gttagatagg caaaaccttg agtaggaaaa cttttcttac cttctttttc atagagataa
     3061 aacttcatcg gattttctat attgcttgtg cgtacgatgg tggggggggt gtaagtaaaa
     3121 ccagctttct cccaaacgtc tttgacaagc gtgtttgtga aaatagaatc gagcacggtt
     3181 agaattttat cggtgctgcg gaaattttgc ggcagttggt cttccaacgc gccataatga
     3241 tgcatttctt gttttgcatt gagatacaca aaaacatctg cgccacggaa tttataaatt
     3301 gcctgtttgg gatcgcccac gacaatgagc ttgtgagttt tatttaaaaa taaattgcta
     3361 aaaattttcc actgtatagt gtcggtgtct tggaattcgt cgatgagagc gtatctgaat
     3421 tttttttgta tttttttgac gaattctttg tcgtctaacg cgtctgctgt tttggttatc
     3481 atcgttgaaa atgtcagcaa gtttttttgc tcacacgctt ttcgcatgcg ctcgaacgta
     3541 caataaattg tccatgcaac gaaatcgtct tctgtctgga cttgctgtat ctcttttata
     3601 tctggaaaaa tttttgtcga tggcgaggca aaaagatgcg cgcttttagc ggtgatttct
     3661 ttttcgaaat ccgaattaag acctcccttg ccgtactcga cttggagtaa aaaattcgct
     3721 aaatcagcgg tgggcaattt tgtccagtcc agtcgaatca cttcttcgag agcatcttgc
     3781 gctaaaattg tatcgtctgc atttttatcg ttactttcca aaaaattgtt gggtagatcg
     3841 cgtagcgcta aaagacaaaa actatgtatt gtcccgatgg cgttttggga gaattcttgt
     3901 agcgcggctt gggcgtgggg ggatgtactt tctttcaagc gttgataaat acgcgattta
     3961 agctcggcag cagccttttc agtaaaagtg acgcacaaaa tttcagcaag tttagctttt
     4021 tcctgttcga taat
//
LOCUS       DAOMPD010000239         4032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9246_length_4032_cov_2.854161, whole genome shotgun sequence.
ACCESSION   DAOMPD010000239 DAOMPD010000000
VERSION     DAOMPD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_4032_cov_2.854161"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLY93_08885"
     CDS             <1..449
                     /locus_tag="PLY93_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP69631.1"
                     /translation="EVISGGSQEVKVGKLSLNARAYMARFNFTGADQEKKCGVLSGGE
                     RNRLHLALALKDGGNVLLLDEPTNDIDVNTVRALEEGLENFAGCAVVISHDRWFLDRV
                     ATHILAFEGDAQVKFFAGGFSEYEADRKKRLGTEPKRFKYKKLIKH"
     gene            complement(463..1194)
                     /locus_tag="PLY93_08890"
     CDS             complement(463..1194)
                     /locus_tag="PLY93_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP69632.1"
                     /translation="MKVLVIEDDEVQHELLKAVFAQVGADALFCKTGEDGLERLKTHF
                     FDIVLLDLGLPGKSGLQVLKTIRENVLTKEIAVIVFTATKTRESLALCMKYGINDYIA
                     KPFQPATFTKKLDNLRRELALKTTVAKEDAAHVILDRMTLVAKFTFGGVFNDDSVRKF
                     LQYYTPAFQNQTKNNEVLLNFAALPNMTAPQLRMFKLITSAIFPKKPLIVAGRSYGPL
                     LGELSDYEDRLFITEEDALEFLNKH"
     gene            1287..3926
                     /locus_tag="PLY93_08895"
     CDS             1287..3926
                     /locus_tag="PLY93_08895"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP69633.1"
                     /translation="MRRFTQKSLSNYLKILYPHLLIIFQWCMRKCGFIFLFLLIPLSA
                     KNLLREDMFVRQGFTHQWLTQWPERTKAIGDWKHIAATTSDNRPLRIGTLGAPFPKPG
                     IYHWPTKTIHEYTAITRFFLLPNELKAPHAWGIRLGSIGDNWQIYLNGALIRQAWHID
                     AENKRITTHRMIRDAVVEIPQHLLVAGENLLAFRLAGDAESAEVGFFTGQPYEVDSLR
                     TLLYERGETIILILLSLYLAVGFYHLLLYSRRRKENYNLYFGLFCLGLFLYLMTRTSI
                     IFDYVQDSTLVQKIEFVVLFNMVSAFLFFVDTLFTRRISRFPKIYAIIAVILSLATIF
                     TSALFNTTILRVWQLIALVGVLYFVVFQLMRTVIREVRTRIKSSTQKSAVLRLLLSLK
                     DALLFSPSGNLFLGILTTMATAVFDILDSMFFATGIALSKYGFAIFVLGIAVMLANRF
                     LKVHNEVEELNATLEKKVKERTHELQESLERVSALKQQQDADYFLTALLLKPLATSTA
                     QSKNIQMEYLVKQKKTFEFRRWKSEIGGDINMAASISLKERPYVVFVNADAMGKSIQG
                     AGGVLVLGSIIRAIIERTRISPVERQYFPERWLRNVVSEIQLVFESFNGSMYVSTVMG
                     LLDEVSGCFYYINAEHPAPTLYRAGETRFIDPEGTYRKLGVPGLSETVRVKVFRLKPN
                     DVVILGSDGKDDLLLETGDAMKRVNEDESLFLKIVMKTKGQLNEIVKQIETTGEVMDD
                     LSLMRISYHAQETQPNRKELSEKVQKQVSECNAEKNYAKAAEELLSYITHFPDDSKAI
                     LLLVKMYRRMGKYDAAIDWAERLRLRAETNLPALQLLTDLYAYTGNLARAKQILRECS
                     LIDAGNPKTEALREALEKKGEAA"
BASE COUNT         1063 a          982 c          906 g         1081 t
ORIGIN      
        1 tcgaggttat ctccggtggc tcgcaagaag tcaaagttgg caaactctca ctcaacgcac
       61 gcgcgtacat ggcgcgcttc aatttcaccg gcgccgacca agaaaaaaaa tgtggcgtgc
      121 tttcaggcgg tgaacgcaat cgattgcact tagcgcttgc gctcaaagac ggcggcaacg
      181 tgttgctttt agacgaacca accaacgaca tcgacgtgaa cacggtgcgt gcgctcgaag
      241 aaggtttgga aaattttgcc ggttgcgccg tggttatctc gcacgaccgt tggtttttag
      301 accgcgtcgc aacacatatt ttggcgtttg aaggcgacgc gcaagtgaaa ttttttgcgg
      361 gaggtttttc cgagtacgaa gccgaccgca aaaaacgcct cggtaccgaa cccaagcggt
      421 tcaaatataa aaagctgatt aaacattagc cactactcgt ggttaatgtt tattcaaaaa
      481 ttccagcgcg tcttcttcgg tgatgaaaag acgatcttcg taatcgctga gttcacccag
      541 taaaggccca taactacgcc ccgcaacgat gagtggcttt ttaggaaaaa ttgcactggt
      601 gattagtttg aacatccgta actgcggcgc agtcatgttg gggagcgcgg caaaatttaa
      661 cagcacttcg ttgttctttg tttggttttg aaacgcaggc gtgtaatact gtagaaattt
      721 gcgcacgctg tcatcgttaa aaacaccgcc aaaagtaaac ttagccacca atgtcatacg
      781 atctaaaata acgtgtgccg cgtcttcttt agcgacggtc gttttaagcg ccaattcacg
      841 gcgtagatta tccaattttt ttgtgaacgt cgccggttga aacggtttag caatatagtc
      901 attgataccg tatttcatac aaagtgcaag cgattcgcgg gttttagtcg ctgtaaaaac
      961 aatcaccgca atttcttttg tgagtacatt ttcccggatt gtttttagca cctgcagacc
     1021 gctttttcca ggaagaccta aatcaaggag cacaatgtcg aagaaatgtg ttttgagccg
     1081 ctcaagaccg tcttcgccgg ttttacaaaa aagtgcatcc gcgccaactt gtgcaaagac
     1141 tgccttcaag agctcgtgct gcacttcatc gtcttcaatg accaaaacct tcattaaaga
     1201 aaaaggggat gcacgataga cagcctgtaa agaatttgtg gataaattat cgctgttttc
     1261 aaaaaatttg atacaccaca atacccgtgc gtagatttac gcagaagtca ctctcaaact
     1321 atcttaaaat tctctatcca cacttactca taatttttca gtggtgtatg cgaaaatgtg
     1381 gatttatttt tctctttctg ttaatccctc tgtcggctaa aaatttatta cgtgaagata
     1441 tgtttgtacg ccagggtttc acccaccaat ggctcaccca gtggccagaa cgcacaaagg
     1501 cgattggcga ttggaagcat attgcagcga ctacaagcga taaccgacct ttgcgtattg
     1561 gcacgctcgg agcgcctttc cctaaacctg ggatttacca ctggcctaca aaaaccatcc
     1621 acgaatacac cgccatcacc cgtttctttt tactgccgaa cgaactgaaa gcgccgcatg
     1681 cgtgggggat taggctcggc agtatcggcg ataactggca aatttatttg aacggcgcgt
     1741 tgatccgcca ggcgtggcat atcgacgctg aaaacaaacg tatcaccaca caccgcatga
     1801 tacgcgacgc ggtcgtggaa atcccgcaac accttttagt cgccggtgaa aatttactcg
     1861 ccttccgtct tgccggcgac gccgagagcg cagaggtagg cttcttcacc ggccaaccgt
     1921 acgaagtgga tagtctacgc accctcctct acgaacgcgg tgaaacgatt atcctcatcc
     1981 tcctttcact ctatttagct gtgggatttt accatctgct cctttattca agacggcgca
     2041 aggaaaacta caatctttat ttcggtttat tttgcctcgg acttttttta taccttatga
     2101 cgcgcacgtc gattattttc gattatgtgc aagattcaac gctcgtacaa aaaatagaat
     2161 ttgtcgtgct ctttaatatg gtttccgcat ttcttttttt tgtcgacacg ctttttactc
     2221 gtcgtatctc tcgtttccct aaaatttatg caatcatcgc ggttatactt tcgcttgcga
     2281 caatttttac aagcgcactt ttcaacacca caattttgcg cgtgtggcaa ctgattgcat
     2341 tagtgggcgt actttacttt gtcgtttttc agctcatgcg taccgtgatc cgcgaggtaa
     2401 gaacacgcat taaatcctca acgcaaaaaa gtgccgtact gcgtcttttg ctctctctca
     2461 aagacgcgct tcttttttct ccttcgggga atttgttttt gggaattctc accacaatgg
     2521 ccacggcggt ctttgatatt ttagattcta tgttctttgc aacggggatt gctctttcta
     2581 aatacggctt cgctattttt gtgctcggca ttgcagtgat gctcgccaac cgtttcctca
     2641 aagtccacaa tgaagtggaa gagctcaacg ccacactcga gaagaaagtc aaagagcgca
     2701 cacatgaact ccaagaaagc ttggagcgcg tttctgcgct caaacagcaa caagacgccg
     2761 attactttct cacagccctc cttttgaaac cgttggccac aagtaccgct caaagtaaaa
     2821 atatccaaat ggaatatctg gttaaacaaa aaaagacatt cgagttccgg cgctggaaat
     2881 ccgaaattgg cggcgacatc aacatggccg cgtctatctc gcttaaagag cgcccctacg
     2941 ttgtttttgt caacgccgac gcgatgggta aatcgattca aggtgcaggc ggcgtgctgg
     3001 ttttaggttc tatcatccgc gccatcatcg agcgaacccg cattagccct gttgaacgcc
     3061 agtacttccc cgagcggtgg ttacgtaatg tagtgagtga aattcaactc gtttttgaga
     3121 gcttcaatgg ctcgatgtac gtgtccaccg tgatggggct tctcgacgaa gtcagcggtt
     3181 gtttttatta catcaacgca gaacaccccg cgcccacctt ataccgtgca ggcgaaacac
     3241 gctttatcga tccggaaggc acataccgta aactgggtgt tcccggactt tcggagacag
     3301 tgcgtgtaaa agtttttcgt ctcaaaccca acgacgtggt gattctcggc tcggacggta
     3361 aagatgactt actccttgaa acgggcgatg cgatgaaacg cgtcaatgaa gatgaatctt
     3421 tgtttctaaa aattgtaatg aagacaaaag gccaactcaa tgagatagta aaacaaatcg
     3481 aaacaactgg tgaagtgatg gacgatcttt cactgatgcg gatttcttat catgcacaag
     3541 agacacaacc caacagaaaa gagctctccg aaaaagtaca aaagcaagtt tccgagtgca
     3601 acgctgaaaa gaattatgca aaggcggcag aagagttact ttcgtacatc actcatttcc
     3661 ccgacgactc aaaagcgatt cttctccttg ttaaaatgta ccgccggatg ggtaaatacg
     3721 atgccgccat cgactgggcg gaacgtttac ggttgcgcgc tgaaacgaat ttgccagctc
     3781 tgcaattact caccgacctt tatgcgtaca cgggtaatct tgcacgggca aaacaaatat
     3841 tgcgggaatg ctcgcttatc gatgcgggta atcccaaaac cgaggcgctg cgcgaagcgc
     3901 tggagaaaaa aggagaggcg gcgtgataaa atttttttac cgcgctgcat ttatacttgc
     3961 cgttgtagtt actctcgttt cttgccaccg ctcgtcggtc gaatggcgca ggctcgattt
     4021 aggtctcacg cc
//
LOCUS       DAOMPD010000240         4026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9270_length_4026_cov_2.816671, whole genome shotgun sequence.
ACCESSION   DAOMPD010000240 DAOMPD010000000
VERSION     DAOMPD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9270_length_4026_cov_2.816671"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1018)
                     /gene="pyrB"
                     /locus_tag="PLY93_08900"
     CDS             complement(89..1018)
                     /gene="pyrB"
                     /locus_tag="PLY93_08900"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRP69634.1"
                     /translation="MSWKKQSILSAGQFNKNDLLEIIALSEKLENALKIKPNLKLLDD
                     YILAALFFEPSTRTRLSFEAAMHRLGGRVISAVGMQFSSLAKGETLFDTLKMVESYSD
                     ICAIRHSVEGASALAAQSIKIPVMNAGDGAGEHPTQGLLDLYTIFKRGFFEKPKLKIA
                     FVGDIRYGRTIHSLVQLLAHFDAEMTFIAPDELQLPDKYKVLLDSAKITYSQTHTIEA
                     LTAVDVGYITRIQEERFVDHREYEKFKDAYIVDADLVGRCQSHIAIMHPLPRLSEVST
                     DLDNHPAAAYFEEAHNGLYVRMALILGLLGVNF"
     gene            complement(1015..>2302)
                     /locus_tag="PLY93_08905"
     CDS             complement(1015..>2302)
                     /locus_tag="PLY93_08905"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRP69635.1"
                     /translation="ELKTQQDGDYFLTSLLIEPLSANRVESNYTKVQFFVKEKKEFSF
                     RRWSRDIGGDMCIAHTVELRGRKFTIALNADAMGKSMQGAGGALVLGSVFASIIERTR
                     SASIVRDSAPETWLRAAFMELNRVFESFDGSMLVSVVLAAIDDEAGVMYYINAEHPWS
                     VIYRNGKAAFTDEDFMLRKLGTLVGNENIQVHVFYLEDNDVVIMGSDGRDDLILGIDH
                     EGHRIINEDETLFLKHVEDAKADLPKLVETLKETGDLSDDLSFIRIEYHAPEHRRAPI
                     RLGDSQAVENFKTNLKTNAKPLTIHDIDIILTRHQHNATEMHYAIRWLIQLKQYEKAG
                     EWAEKFATEFPMETEFLYLAALGYKLAGRYGEALDFSERLFVRIASHEKNLINLVDLQ
                     ILLKRKGDAENALSFLNKYFPHNEKIVKLKQALEQI"
     assembly_gap    2303..2312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2313..3653)
                     /locus_tag="PLY93_08910"
     CDS             complement(<2313..3653)
                     /locus_tag="PLY93_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRP69636.1"
                     /translation="MRFLLRYVLIAFSFAVIPLSAAENFPVQVIDMGNTNATVSLESG
                     AKNASTWFVSDKPVDPMAFSSQMLKGEKRKEATPQSFTPVAVPSNLVKLFGEKKVGET
                     IWYYKSFIAPSNPLATLSLRLGKINDADEVYLNGILIGKSGSTSLFDHAWDKVRVYDI
                     PSSLLRPLEKNIILIRVHVYFSNEYGIIRDHVQIGDSEIMNRELIVNDILVLVFLSCY
                     LTFGLYFLFLFLRRPTEKSYLLFSLFIFTLVLYQFLQTQVKYYVSANFLALKRIEYLA
                     LFSIFPAFYFYLREFFQVKTNKVFRYIHWGLYAFFAVIVVLYGFVLLVDNPLAWTSWN
                     ERVYLKYIMPVLITVSFAIVVYKIFKRDKNARAIMVGLFFLLVTLVIDNLVYYGIINV
                     PRVTSYVIFTFIMSLAFILANQFVRVHKQVEDLNRNLEKKVEERTQELRESLTRV"
     gene            3745..3945
                     /gene="rpmE"
                     /locus_tag="PLY93_08915"
     CDS             3745..3945
                     /gene="rpmE"
                     /locus_tag="PLY93_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRP69637.1"
                     /translation="MKAGIHPVYEDAVIRCACGAEHKTRSTKKEIFVEICSNCHPFFT
                     GKTKLVDTAGRVDKFKKKYKMK"
BASE COUNT         1166 a          925 c          856 g         1069 t
ORIGIN      
        1 ggtcgaaaag gagggtaaat tttgaggaaa agctctcttt caggtgggta ggccaccgct
       61 tcttcgcgtc acgaaggcca taaccctgct aaaaattaac acccaacaat cccaaaataa
      121 gagccatgcg cacgtaaagc ccattgtgcg cctcttcaaa gtatgcagcg gcagggtggt
      181 tgtctaaatc ggttgagacc tcactcaaac gtggcagtgg atgcataatc gcaatgtgcg
      241 attgacaccg accgactaag tccgcatcga cgatatacgc gtctttgaat ttttcatatt
      301 cacggtgatc gacaaagcgc tcttcttgga tacgagtaat ataccccacg tccactgccg
      361 tgagcgcttc aattgtgtgc gtctgtgaat acgttatctt tgctgaatca agaagaactt
      421 tatatttatc gggaagttgt aattcatcgg gagcgataaa agtcatctcg gcgtcaaagt
      481 gcgctaagag ttgcactaaa gagtggattg tgcggccgta acggatgtca cctacaaaag
      541 cgattttgag cttaggtttc tcaaaaaatc cacgtttaaa aatggtgtaa aggtctaata
      601 acccttgcgt gggatgctca cccgcgccgt cgcctgcatt catcactgga attttaatac
      661 tttgtgccgc caacgccgat gccccttcta ccgagtggcg tattgcgcaa atgtcactat
      721 acgattcaac catctttaat gtatcaaaga gggtttcacc ttttgccaaa gacgagaact
      781 gcatccccac tgcagaaata acacgtccgc ccaagcggtg catcgccgct tcaaaagaga
      841 ggcgtgtgcg tgtcgacggt tcaaaaaaaa gcgccgccaa aatataatcg tccaaaagct
      901 ttaagttcgg tttaattttg agtgcatttt ccaatttttc actcaacgca ataatttcaa
      961 gtaaatcgtt cttgttaaat tgccccgctg atagtatcga ttgttttttc caactcatat
     1021 ctgttcaagt gcctgtttta attttacaat tttttcgtta tgcggaaaat atttattcaa
     1081 aaacgataac gcgttttcgg catcgccttt ccgtttcaat aaaatttgca agtcgactaa
     1141 gttaatcaaa ttcttctcat ggctggcgat gcgcacaaaa agtcgttctg aaaaatcaag
     1201 cgcttcgcca taacgccccg ctaacttata gcccaacgcg gcgaggtata aaaactcggt
     1261 ttccatagga aactcggttg caaatttttc tgcccactct cctgctttct catattgctt
     1321 gagttgaatt aaccaacgga tggcataatg catttcagtg gcgttatgct gatgcctcgt
     1381 tagaatgata tcgatgtcgt ggatcgtcaa cggctttgca ttcgttttca agttcgtttt
     1441 aaaattttca accgcctgcg aatctcctaa gcggataggc gcacggcggt gttcgggcgc
     1501 atggtattca atgcggataa acgaaaggtc gtcactcaaa tcgccggttt ccttcagcgt
     1561 ttcgacgagc tttggtaaat cggcttttgc atcttctaca tgtttcaaga atagagtttc
     1621 atcttcgttg atgatgcgat ggccttcgtg atcaatcccc aaaataagat catcgcgccc
     1681 atccgagccc ataatcacaa catcgttatc ttctaagtaa aaaacatgca cttgtatatt
     1741 ttcattgccg acgagagtgc ccaatttacg taacatgaaa tcctcgtcgg taaaggccgc
     1801 tttaccatta cggtaaataa ccgaccatgg atgttcggcg ttgatgtaat acatcactcc
     1861 cgcttcgtcg tcgatggcgg caagcaccac agaaacgagc atcgaaccgt cgaagctttc
     1921 aaaaacacgg ttaagttcca taaacgcggc gcgtagccat gtttcgggtg cgctgtcgcg
     1981 tacgatgctt gcgctgcgtg tgcgctcgat aatgctcgca aataccgagc ctaaaacaag
     2041 cgctccaccc gccccctgca tcgacttgcc catcgcgtcg gcgttgaggg caatggtaaa
     2101 tttacgccca cgcaattcga cagtatgcgc gatgcacata tcgccaccga tgtcgcggct
     2161 ccatcttcta aacgaaaatt cttttttctc ttttacaaaa aattgaactt tagtgtaatt
     2221 actctcgaca cggtttgccg acaacggctc gatcaacagt gaggtcaaaa aatagtcccc
     2281 gtcttgttgc gttttaagct ctnnnnnnnn nncacgcgcg ttaacgattc tcgaagttcc
     2341 tgcgttctct cttcgacttt tttttccaag ttacggttta aatcttcgac ttgtttgtgc
     2401 acgcgcacaa actggttggc taaaataaac gccaaggaca tgataaatgt aaaaattaca
     2461 tagctggtta cacgcggtac attgatgatg ccgtaataga ctaaattgtc aattaccagc
     2521 gttaccaata agaaaaacaa tcccaccata attgcacgcg catttttgtc gcgcttaaaa
     2581 attttataga caacgatggc aaaagagacg gtgatgagga cgggcataat gtatttgaga
     2641 taaacccgct cattccacga tgtccacgcg agtggattat cgaccagaag aacaaaaccg
     2701 tagagaacaa caattacagc aaaaaaggcg tacagccccc aatggatata acgaaacact
     2761 ttattcgttt tgacttgaaa gaactctctt aaataaaaat aaaatgccgg aaaaatggag
     2821 aacagcgcca agtattctat ccgttttaat gccagaaaat tagctgaaac ataatacttc
     2881 acttgcgttt gtaaaaattg atagagcacc agagtgaata taaagaggct aaaaagtaaa
     2941 tacgacttct cggttgggcg gcgaagaaac aaaaaaagaa aatacagacc aaaagttaga
     3001 taacacgaaa gaaaaacaag gacaagaata tcgtttacga tgagttctcg attcatgatt
     3061 tcagaatcgc cgatttgaac atggtcgcgg ataatgccgt attcattcga gaaataaaca
     3121 tgcactcgaa tcaaaataat atttttttcc aaaggtctaa gtaacgacga agggatatcg
     3181 tacacacgca ctttatccca cgcgtggtcg aaaagcgatg tgctgccact tttgccaatt
     3241 aaaattccgt tgagatacac ctcgtcggca tcgttgattt tacccaaccg caaagacagc
     3301 gtggccagcg ggttggaggg cgcgatgaac gatttgtaat accaaatcgt ctcaccgact
     3361 tttttttcgc caaacagttt tacgagattg gatggaacag caacgggggt aaacgattgg
     3421 ggtgtagctt cttttctttt ctctcctttg agcatttggc tcgaaaaggc catgggatcc
     3481 acaggcttgt cgctcacgaa ccaggtcgat gcgttcttcg cgccgctttc taaagaaacg
     3541 gtggcgttgg tattccccat gtcgatgact tgcaccggaa aattttcagc agcagagagc
     3601 gggattacag caaacgaaaa tgctatcaag acgtatcgga gtaaaaatcg catatacgaa
     3661 tgtttgtgcg tgtattcgtc gcgtcaagcg taagcgtatt tgaaaaagct tgtttgcctg
     3721 tctcgctcgt aaactctgcc acttatgaaa gcaggcatac atcccgttta cgaagacgcg
     3781 gtgatccgct gcgcgtgcgg cgcagaacac aaaacacgct caaccaaaaa agaaattttc
     3841 gtggaaattt gttccaattg ccaccctttc tttaccggca aaacaaagct ggttgacacc
     3901 gctggccgtg tcgacaaatt caagaaaaaa tataaaatga aataactgtt tatattttta
     3961 cgcgccgacg tgtaaaaata taaacaaact cagggcggcg taaacgccgt ccgttcgagc
     4021 ctaaat
//
LOCUS       DAOMPD010000241         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9338_length_4005_cov_2.640506, whole genome shotgun sequence.
ACCESSION   DAOMPD010000241 DAOMPD010000000
VERSION     DAOMPD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_4005_cov_2.640506"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLY93_08920"
     CDS             complement(<1..477)
                     /locus_tag="PLY93_08920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP69638.1"
                     /translation="MPQKTVLITGATRGIGRGIAVGFADAGYQVYGTGRSVTSLTAEP
                     SGWIKEKGIILKAADAADQASMAAFMKEIHTTHGRLDCLVNNAGIAANTPASRITLDE
                     ANRIIDTNFKAVFQNAQSYYQLQRKEGGNIINIASVLGMVGTTLASVYSGTKGAVIA"
     gene            complement(495..1637)
                     /locus_tag="PLY93_08925"
     CDS             complement(495..1637)
                     /locus_tag="PLY93_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRP69639.1"
                     /translation="MSALTILSDEEQSYFDAIKDFAKKEIAPHVSAMDEAQKINPDIV
                     KKLFEMGLMGIEVPQKLEGSGASFFTAILAVEGISQVDPSVGVMVDVQNTLVNNAFMR
                     WGSDALKKKYLPQLATGKVGSYCLSESSSGSDAFALKTKAEKKGDKYILNGQKLWITN
                     AGEADIFIIIANLAPEQGYKGITAFIVERGFKGFSVSKKENKLGIRASSTCELVLDNC
                     EVPLENLLGEEGKGYKVAIETLNEGRIGIGAQMVGLSEGALQHAVDYVKTRQQFGKPI
                     SSFQGMQFQLAQMAIDVESAKLMVYNAARLKDAGRDFVKEAAMAKFHSSQVAERVASL
                     AVECFGGYGFVKDYPVEKLYRDAKIGKIYEGTSFMQLMTIGKILLG"
     gene            1749..3461
                     /locus_tag="PLY93_08930"
     CDS             1749..3461
                     /locus_tag="PLY93_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRP69640.1"
                     /translation="MISHIYYESQFSRISLETFRDTIHFAAIENNLAIRQQQPDEEHA
                     VVWLLSAKGLAEHSTFIANWLQKYPQHRARIIALIDHDITDGQFFPLRDEQVSLCLSS
                     NMSMRQVSKAVEVQFNAIQAEFDRLNLQERLANSYIEIRRLTAVGQALATERDFDKLI
                     ELVLNSAIELTNADGGSIYLSEKHGDSPIVSHIRFKKSALQLDADEFLLPIDKTSIAG
                     YVALTVEPLLIDDVHNLPKNADYKYNSEYDRAHKYYTKSMMVLPMKNHHDEVIGVIQL
                     INRKRDADLKLSLEELKGNAVIPFTRKDYSIVSAMAGQAAVAIENNQLMRDIQNLFEG
                     FVQASVIAIEQRDPTTSGHSFRVADYSVNIAQAVNRDVSTRFSEVRFSRNQIQELRYA
                     SLLHDFGKVGVREDVLVKAKKLYPEQLELIRWRFHYIRKDIESKLLASTMVKLKAAHP
                     QEWPEIEALFQIELEKQLQEAEEMFSTILSANEPTVLAEGSFEFLKKIAERKIELTKE
                     ATMPFITNEELVMLSIRKGTLDENERKEIESHVTHTYNFLKQIPWTPNLRSVPEITYG
                     HHEN"
     gene            3507..3728
                     /locus_tag="PLY93_08935"
     CDS             3507..3728
                     /locus_tag="PLY93_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69641.1"
                     /translation="MQTRMMTIADIYDALTAWDRPYKKAIPPEKALDILQMEVKDGHV
                     DNDLLRIFIEAKVFERIDKLRAASLEHVR"
     gene            3775..>4005
                     /locus_tag="PLY93_08940"
     CDS             3775..>4005
                     /locus_tag="PLY93_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69642.1"
                     /translation="MRYFTLLILVFVAVSCGKKKEKTADAAQPRIAYAVYGVGIFDQI
                     GQTKASVYLNRGEMLTVLDTVETNVLLIIVSSV"
BASE COUNT         1032 a          987 c          958 g         1028 t
ORIGIN      
        1 cgcaatgacc gcacccttgg taccagagta caccgacgcg agcgttgtgc ccaccattcc
       61 caatacgctt gcgatgttga tgatgttgcc gccttctttg cgttgtagtt gataataact
      121 ttgtgcgttt tgaaacaccg ctttgaaatt agtgtcgatg atgcggttgg cttcgtcgag
      181 ggtgatgcgc gaagcggggg tgttcgcagc gatgcctgcg ttgttcacaa ggcaatccaa
      241 ccgtccgtgc gtggtgtgga tttctttcat aaacgcagcc atacttgcct gatcggcggc
      301 atcagcagct ttgagaataa tccctttttc ttttatccac cccgacggtt cggcagtcag
      361 cgaggttacc gaacgccccg ttccataaac ttggtaaccg gcgtcggcaa aacccacagc
      421 aataccgcga ccaatgccgc gtgtcgcgcc cgtgatgagg actgtttttt gtggcatcga
      481 tgcgcgctct ggggttatcc caacaaaatc ttgccgatgg tcatgagctg cataaagctc
      541 gtgccttcgt aaattttgcc gatctttgcg tcgcggtaca gcttttcgac ggggtagtct
      601 ttcacgaagc cgtaaccgcc gaagcactcg accgcaagcg aggcgacgcg ctcggcgact
      661 tggctcgaat ggaattttgc catcgccgct tctttcacga aatcgcggcc tgcgtctttc
      721 aggcgcgcgg cgttatagac catgagcttg gccgattcga cgtcgatcgc catttgcgcg
      781 agctgaaatt gcatgccttg aaaagaagat atcggtttac cgaactgttg gcgcgttttc
      841 acataatcga ccgcatgttg caatgcgcct tctgaaagcc ccaccatttg cgcaccgatg
      901 ccgatgcgcc cctcgttcaa ggtctcaatc gcgactttgt atcccttgcc ttcttcgccg
      961 agcaaatttt ctagcggcac ttcgcagttg tcgagcacga gttcgcaggt cgacgaagcg
     1021 cggataccca acttattttc ctttttcgag acagaaaaac ctttgaaacc acgctcgacg
     1081 ataaacgccg tgatgccttt atagccctgt tcgggggcga ggtttgcaat tataatgaag
     1141 atatccgcct cgcccgcgtt ggtaatccac agtttttggc cgttcaaaat atatttgtcg
     1201 cccttctttt cggctttggt cttgagagca aacgcgtcgg aacccgacga actttcggaa
     1261 aggcagtacg aaccgacttt gccggtcgct agttgcggca aatatttttt cttgagtgca
     1321 tccgagcccc agcgcatgaa cgcgttgttg acaagcgtgt tttgcacgtc gaccatcacg
     1381 ccgacagacg ggtcgacttg tgaaatacct tctaccgcca aaattgcggt aaaaaacgac
     1441 gcgcccgagc cctcgagttt ttgcggcacc tcgatgccca tgagccccat ttcaaaaagt
     1501 tttttgacaa tatcggggtt aattttttgc gcttcgtcca tcgctgacac atgcggagcg
     1561 atttcttttt ttgcaaaatc tttgatggcg tcaaagtacg attgttcttc gtctgaaaga
     1621 atagttaatg ctgacatggg taaatctgcg gtatttattc agtgtggaaa gaattattgg
     1681 gggattagga acctgtgaac atttactatt ttgtgttgac ccatcgtatc cacaagcgcg
     1741 gctgaaacgt gatttcgcat atctactatg aaagtcaatt ttctcggatc tcattagaaa
     1801 cctttcggga cacaatacac tttgccgcca ttgaaaataa tttggccatt cgtcaacaac
     1861 aacccgacga agaacacgcc gttgtttggt tgttgtcggc aaagggtttg gcagaacact
     1921 caacgttcat tgcaaactgg ttacagaaat acccgcagca ccgtgcgcgt atcattgcac
     1981 tcatcgatca cgatattaca gatgggcaat tctttccctt gcgagatgaa caagtcagtt
     2041 tatgcttgtc ctcaaatatg tcgatgcggc aagtgagtaa agccgtcgaa gttcaattca
     2101 acgccatcca agcagagttt gaccgactca atttacagga aaggctcgcg aatagctaca
     2161 ttgaaatccg ccgattgaca gcggtgggac aggcgttggc gaccgagcgt gattttgata
     2221 aacttatcga gttggtgctc aattcggcaa tcgaattgac gaacgccgac ggtggatcca
     2281 tttatttgag tgaaaagcac ggtgactctc cgatagtttc gcatatccgc ttcaagaaat
     2341 cggcgttgca gttagacgct gacgagtttt tactccccat cgataaaact tcaatcgctg
     2401 gttatgtggc gctcactgtg gagccgcttc tcatcgacga cgtgcataac ttgcccaaga
     2461 acgccgacta taaatacaac agcgaatacg acagagcgca caaatactac accaagtcga
     2521 tgatggtgct gccgatgaaa aatcatcacg acgaagtcat cggcgttatt caactcatca
     2581 atcgcaaacg cgacgccgat ctcaagctct ctttagaaga actcaaaggc aatgcggtga
     2641 ttcccttcac tcgcaaagat tattctatcg tgagcgcaat ggccggccaa gcggctgtgg
     2701 cgattgaaaa taaccagctc atgcgcgaca ttcaaaacct tttcgaaggt tttgtacaag
     2761 cgtcagtgat tgccatcgaa cagcgcgatc ccaccacgag tggccattcg tttcgtgtcg
     2821 ccgattactc ggtgaatatc gcgcaagcag tgaaccgcga cgtttcaacg cgctttagtg
     2881 aggtacggtt ttcacgcaac caaattcaag agctgcgtta tgcatcgctc ttgcacgact
     2941 ttggtaaagt cggtgtgcgc gaagacgtgt tggtcaaagc caaaaaatta tatcccgaac
     3001 agttagagct tatccgttgg cggtttcact acatccgtaa ggatattgaa agcaaattgc
     3061 tcgcatcgac gatggtgaaa ttgaaagcag cccatccaca agaatggccg gagattgagg
     3121 ctctctttca aattgaatta gaaaagcaac tccaagaagc cgaagaaatg ttttcgacga
     3181 ttttgtctgc gaacgaacca acggttcttg ccgagggatc gtttgagttt ttgaaaaaaa
     3241 ttgccgaaag aaaaattgaa ctcacaaaag aagcgaccat gccttttatt accaatgaag
     3301 agttggtgat gctctcgatc cgcaaaggca cactcgacga aaatgagcgc aaagaaatcg
     3361 aatcacacgt cacgcacacg tataattttt tgaagcaaat accgtggacg cccaacttgc
     3421 gttcagtgcc cgagattaca tacggccacc atgaaaacta aaacggcgca tgatatccac
     3481 tccaccgcca cgaaaaagaa atctcgttgc aaacacgaat gatgaccatc gccgacatct
     3541 acgacgcact caccgcgtgg gatcgtccgt ataaaaaggc aatcccaccg gaaaaagcgt
     3601 tggatatttt acagatggaa gttaaagacg ggcatgtgga taacgatttg ttgagaattt
     3661 ttatcgaagc gaaagtcttt gaacgtatcg ataagttgcg cgccgcttct ttagagcatg
     3721 tgcgttgatt ttctttattg acatagtatc tcattcagag ccacgcctca tgccatgaga
     3781 tattttacac tacttatttt agtttttgtc gctgttagtt gcggtaagaa aaaagaaaaa
     3841 accgccgacg cggcgcaacc gcgcattgca tacgcggtgt acggtgtggg gatttttgat
     3901 caaattggtc aaaccaaagc gagcgtatac ttgaaccgtg gcgaaatgct caccgtctta
     3961 gacaccgtcg aaacgaacgt gctgttgatc atcgtgtcga gcgtc
//
LOCUS       DAOMPD010000242         3998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9370_length_3998_cov_2.705554, whole genome shotgun sequence.
ACCESSION   DAOMPD010000242 DAOMPD010000000
VERSION     DAOMPD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9370_length_3998_cov_2.705554"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLY93_08945"
     CDS             <1..423
                     /locus_tag="PLY93_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP69643.1"
                     /translation="FMALPTIASIADESLQAVPRNLKEASLALGANRIQTILRVTFPA
                     ASSGIIAGVMLGVGRVLGETMTVMMVAGGSAQIALSPMAPVRTMTGTIASEMGEVVTG
                     DVHYRALFMIGLLLFVITLTINTLSGIIIERIRQRQGV"
     gene            420..1277
                     /gene="pstA"
                     /locus_tag="PLY93_08950"
     CDS             420..1277
                     /gene="pstA"
                     /locus_tag="PLY93_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRP69644.1"
                     /translation="MIKRTTRIGDVFGYAVIYSLTAITFIPLLAFIVYIVAKGIGRID
                     LDFLFGASSSLESGGIFAPLYGTIILTLLSLLFSVPIALFAAIYLSEYARKNRWFAII
                     ELTIVNLAGVPSVVYGLFGLGLFVVFFGFGKSLLAGALTMACMSLPLLITVFYQALKA
                     VPNTLREASLSLGASKLATAFRVVVPTATPGIFTGVILATARIAGETAPVLFTVAAYY
                     MPQLPQSVYDQSMLLSYHLYALSTQNSAASEEQKFAVTLVLVTLVLLVNALAILGRAR
                     ARKKIGNLE"
     gene            1281..2057
                     /gene="pstB"
                     /locus_tag="PLY93_08955"
     CDS             1281..2057
                     /gene="pstB"
                     /locus_tag="PLY93_08955"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRP69645.1"
                     /translation="METQNETAPILTLRNLNMRYGEKKALTDINMDILPRSITAFIGP
                     SGCGKSSLLRCFNRMNDLIENALVTGEILFDGQLNINDAKIDVTQLRRKIGMVFQKPN
                     PFPKSIYENIAYGIRVAAKSTKSEIDDRVEDALKKVGLWKEVADRLKDAGTRLSGGQA
                     QRLCIARTIATRPEIILMDEPTSALDPLSTQTVEELTLELKENYTVVIVTHNMQQAAR
                     ISDNTAFFLHGELIEYSPTKLMFTRPNNKRTEDYITGRFG"
     gene            2115..2765
                     /gene="phoU"
                     /locus_tag="PLY93_08960"
     CDS             2115..2765
                     /gene="phoU"
                     /locus_tag="PLY93_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRP69646.1"
                     /translation="MLIIEKALKQLRENLHAMTEVVIDMLEMVVVSLDKGNLEDLENI
                     FKQDKYLDQLEKSLDRQSMELLALINPHASDLRFVFSVIKTNGNLERMGDEMKNVARE
                     AKNLKGKIPDELKELARLVFEMSKKTFLALAQSDAKLSMNIILEDDTIDTLEYNIVTK
                     YPQLDIALTAKALERIADHATNIAESIIYSVEGVDVRHENTIKQRAFEKEYTSPLP"
     gene            complement(2889..3425)
                     /locus_tag="PLY93_08965"
     CDS             complement(2889..3425)
                     /locus_tag="PLY93_08965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRP69647.1"
                     /translation="MANSVEKRATVRGMFNRIAPTYDLLNHLLSFGIDRRWRKATIQL
                     LKLRRDSVLLDLACGTGDLSEEATKHEVRTIFAVDPSRQMLHRAEAKLRYNTTRCYFL
                     ESFGEELPLKDNLCSHAMIAFGIRNVAERNYAFKEIYRVLKPGGYFAILEFTPMEKKS
                     PHSFLIFIFKKFYLPLAL"
BASE COUNT         1113 a          962 c          874 g         1049 t
ORIGIN      
        1 ttcatggcgc tgcccaccat tgcgagcatt gccgacgaat cactacaagc cgtgcctaga
       61 aatctcaaag aggcctcttt ggcgctggga gcaaaccgca tacagacaat cctacgcgtc
      121 actttcccgg ccgcttcttc cggtattatc gccggcgtta tgttgggggt gggacgcgtc
      181 ttaggagaaa caatgacggt catgatggtc gccggtggtt ctgcgcaaat tgcactttcg
      241 cccatggcgc ctgtgcgcac gatgactggc accatcgcct cagagatggg tgaggtggta
      301 accggagacg tgcactaccg tgcacttttt atgatagggt tgttgctttt tgtcatcaca
      361 ttgactatca atacattgag cggcatcatc attgagcgca ttcggcagag gcaaggcgta
      421 tgataaaacg cacgacacgt atcggtgatg tcttcggcta cgccgttatc tactcgttga
      481 ccgcaattac atttatcccg cttttagcgt ttatagttta tatcgttgcc aaaggcatcg
      541 gccgcatcga tttagatttc ctcttcggcg catcgtcttc tttggaaagt ggcggcattt
      601 tcgcgccatt atacggtaca ataatactaa cgctcctgtc gcttttattc tctgtcccca
      661 ttgcactttt tgcggcgatt tatttatccg agtacgcccg taaaaatcgc tggtttgcca
      721 tcatcgaatt gacgattgtg aacttagcgg gagtaccctc ggtggtgtac ggtcttttcg
      781 gactcggctt attcgtcgtg ttcttcggtt ttggtaaatc gttattggcg ggtgccctca
      841 ccatggcttg catgtcgctt cccctcctta ttactgtctt ttatcaggca ctcaaagccg
      901 tacccaacac actcagggag gcgtcgcttt ccctcggcgc aagcaaactg gcgacggcgt
      961 tccgcgtcgt ggtgccgaca gcaacaccgg ggatattcac cggtgtcatc cttgcgaccg
     1021 cgcgtatcgc cggtgaaacc gcgcccgtcc tttttaccgt ggcggcgtat tacatgccac
     1081 agttgccaca aagcgtttac gaccaatcga tgctcctctc gtaccatcta tacgctttaa
     1141 gtacgcaaaa cagcgccgcc agcgaagaac aaaaatttgc cgtcactttg gtgctcgtca
     1201 ctctggtttt actcgtcaac gcgcttgcaa ttttaggccg cgcccgcgcg cgcaaaaaaa
     1261 ttggaaatct ggaataatat atggaaacac aaaacgaaac agcccctatc ttaacgctgc
     1321 gcaatttgaa catgcgctac ggtgaaaaaa aggccctcac cgacatcaat atggatattt
     1381 taccgcgctc catcaccgcc ttcattggac cttccggttg cggtaaatct tcacttttgc
     1441 gctgctttaa ccgcatgaac gacttaattg aaaacgcatt agttaccggt gaaatccttt
     1501 tcgacggcca actcaacatc aacgatgcga aaattgacgt cactcagttg agacgtaaaa
     1561 ttggtatggt ctttcaaaag cccaaccctt ttccaaagtc gatttatgaa aacattgcgt
     1621 atggcatccg tgtcgccgcc aagagtacaa aatcagaaat cgacgatcgt gtcgaagatg
     1681 ctctgaaaaa agtgggttta tggaaagaag tcgccgaccg cttaaaagat gcggggaccc
     1741 gtctttcagg cgggcaagcg cagcggctat gtatcgcccg caccatagcg acacgccctg
     1801 aaattatttt gatggacgaa ccgacgagcg ctttagaccc tttgagcacg caaacggtcg
     1861 aagagctcac gctcgaactc aaagaaaact acactgttgt catagttact cacaacatgc
     1921 aacaggcagc acggatttca gacaacaccg cattcttttt acacggcgag cttattgagt
     1981 acagcccaac aaagctgatg ttcacccgcc ccaacaacaa acgcaccgaa gattatatca
     2041 cagggagatt cggatgagat tcaaagcaag cacaattcca aaattcgcta cactttcaga
     2101 aaggagtctt tcgtatgtta attattgaaa aagcgcttaa acaattacgc gaaaatttgc
     2161 acgcgatgac agaggtcgtc atcgacatgc tcgaaatggt cgtcgtttca cttgacaaag
     2221 gaaatttaga agacttagaa aatattttca aacaggataa gtatttagac cagcttgaaa
     2281 aatctctcga ccggcaatcc atggagcttt tggcgcttat caacccccac gcttcagatt
     2341 tgcgctttgt cttttctgtt atcaagacaa acggcaatct ggagcgcatg ggcgatgaaa
     2401 tgaaaaacgt cgcgcgtgaa gcaaaaaatc tgaaaggaaa aatccccgat gaactgaaag
     2461 agttggcgcg ccttgttttt gagatgtcta aaaagacttt tcttgcattg gcacaaagcg
     2521 acgcaaagct gtcgatgaat atcattttag aagacgacac catcgacact ttggaataca
     2581 acattgtcac aaaatatcct caactcgata ttgctttaac ggcaaaggct ctcgagcgta
     2641 tcgccgacca cgccaccaac atcgcagaga gcatcatcta ttctgtcgaa ggggtcgatg
     2701 ttcggcacga gaatacaatc aaacaacggg cttttgaaaa agaatacacg tcgccactcc
     2761 catagagaaa gcgcgagttt ccaacgtcgt aaagccagcg ctttcgcatt cgtggattaa
     2821 atcttcgaca gtgacaaaac gctctaccga ctctggcaaa tatctgtacg cttctttatc
     2881 gcggctaatt aaagcgccaa tggaaggtaa aattttttga aaataaatat taaaaaagaa
     2941 tgcggggatt ttttctccat tggagtaaat tcaagaatgg caaaatatcc ccctggtttt
     3001 agcacgcgat aaatttcttt aaatgcgtag ttgcgttcag caacattgcg gataccaaaa
     3061 gcaatcatcg cgtgagaaca aagattatct ttaaggggca actcttcgcc gaacgattct
     3121 aaaaagtagc accgcgtcgt gttgtaccgt aatttggcct ctgcacggtg cagcatttgc
     3181 cgcgaagggt caacagcaaa aatggtacgt acttcgtgtt ttgttgcttc ttccgaaagg
     3241 tctcctgtac cacacgctaa atcgagaagt accgaatctc ttcggagctt aaggagctgg
     3301 attgttgctt tgcgccacct gcgatcgatg ccaaaggaaa gtaggtggtt tagaagatcg
     3361 tacgtcggag caatgcggtt aaacataccg cgtacagtag cgcgtttttc cacactattt
     3421 gccatgcgta tgggcaactt aaaaaatggc aacgtatcgt cattcattga gcttcgtcgt
     3481 tgtcggcgta tttcgattaa aaactgagac agtctattgc tattaaattg ccgaaattaa
     3541 ggatagtcat ggatataaat tcacgcccgt tttcttttgt cttcgttatg gctttcgtgt
     3601 cgctttacag tagcatgaca tgccttcgtt cgaacattaa aaatataaat cctatagtgc
     3661 aaaattttag taacaaaaca agtgctcaaa tcttcacaac caaaacaagt ggagaaacta
     3721 ccgcacttgc aaatatcgtc tcagaaactc ataatacccc gaaagaagct caagaaaaaa
     3781 attctataca gaaaaaaata aattccagag taaatcagac aaatagcaca ttatttcctt
     3841 ttcttaaaaa ataacgtgag tttcgtttat cgtgggcgaa tttccccata aagcgtcctg
     3901 ctttcattaa ggggatttat cttactaatg caaccgctat tagcaggatg ttgaaaatct
     3961 cctaactttt gtgtatattg accgtatgcg cggtgaca
//
LOCUS       DAOMPD010000243         3997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9371_length_3997_cov_2.801624, whole genome shotgun sequence.
ACCESSION   DAOMPD010000243 DAOMPD010000000
VERSION     DAOMPD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9371_length_3997_cov_2.801624"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLY93_08970"
     CDS             <1..544
                     /locus_tag="PLY93_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69648.1"
                     /translation="FRKKNAGIEVRSFTSEKNHTLPQLVNQKAARLSSEYTQVRLIEE
                     RDSKFKENLHLSVWEIRTRNKVLREETAIVMANEGPVIVSCLIPKADEQQYRTICDNA
                     FYSVVLDASGEAVMPAQKTAKSSVVDFLTRLYFLHVPNNLPSIPPETIFDPPSQQNTV
                     VPPRPIQYDGNYILPNDNPK"
     gene            528..1610
                     /locus_tag="PLY93_08975"
     CDS             528..1610
                     /locus_tag="PLY93_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRP69649.1"
                     /translation="MIIRNKTLPLLVFVALTLSPQNAKMPYQSTLPEALANTTPALEE
                     IWGEDSCETVIVKKKGYPFVSIACRGNYTKAMRTRFVDTLLKNFFTIKRETYKLKPQN
                     GLYLYKTQYGNEIVYIKLFVREAFDLWPKTPVDGKQIALYIQNVRESRDLVKWRTLGV
                     PLTFGVTVGRGNSRDLIGQISSYGDEVWLAVPLEDETIDVADGRLLTITEALDESKIG
                     EYFNELALETSVTGISPLYCSRFCNNIPALRALFGAVQALNESQKLVVLDTMPTPNAS
                     PFYQTARIMNFRAFRAHPFLSGEPQACTHLQNFAAEGGENATRIIAIDAGDENAYNCL
                     SRIAQTNTRTVDFVKISALSLTNRFR"
     gene            complement(1590..3074)
                     /locus_tag="PLY93_08980"
     CDS             complement(1590..3074)
                     /locus_tag="PLY93_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69650.1"
                     /translation="MFKKYIVFFIFFFSLNRIEAKLYLLLDEELRNNKVEQVIYKFVP
                     LTSAQEVELDYVKDFLREERAVAFAERLKKGDHAIYIGSDEWPKDAYIDKLAKDKAYQ
                     QKTENITFLVKNAFAQESVQLPKGFFDRSDLWRKYAEFTHHAYAGRFSLHYLDSRPNV
                     PKAGDWSYFTCVHESGRVMPSQKAPIFLSKKEFRLNFKTDVQLAFLGHLICINTPKAN
                     AIFEVDISKTDIQWSVEASRFSFWNEAVPIRFVLNADKPIYAPLELEFIPEVNETQTE
                     FFFQGERITCTSKRCRKKSARFLLPQFENTHAEFIVAVRALPDAYFRGTVRLLAGSLE
                     LQRVPLVVRPHSWVAELTYALKNPSEYRNAFVFSLIGVVFALLLFYYSFKLIRRRIES
                     VLEQRRKSVAKQTNAAVAIEKNSTVFLTASKNPFGCELYGFGSVVTLRIGEEAATISY
                     AQTTETLPLEQFLHRLPDGYVLTLKKNDSGGLTLDAALLAKSIR"
     gene            complement(3067..3807)
                     /locus_tag="PLY93_08985"
     CDS             complement(3067..3807)
                     /locus_tag="PLY93_08985"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRP69651.1"
                     /translation="MFERIGRGISLPLNSISYITEKKLWGYTVFPLLLTAGVAALLGF
                     LFWFFLLSSITAWLQVDTVNWFFLLRWFYYLFEFIFKLVFFYFLFSFMLRVYLVLFSV
                     IVIPFLSPVIEKILHAEGIDTLPIKASEIFGYIVSTVWYSIKMLLLQGGVALLLLFTG
                     PLQPFLNFFSSSYFIGRSYFDLVFDLVGRPREFSGMIQGNKAEATGLGVFSSVFVFIP
                     VVGVILAPLLCVVAATRLFAEKKTKQVV"
     gene            complement(3860..>3997)
                     /locus_tag="PLY93_08990"
     CDS             complement(3860..>3997)
                     /locus_tag="PLY93_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRP69652.1"
                     /translation="ENIAAQKIFSTYENILSMGMSSDFKLAIQEGATHLRIGSAIFGA
                     R"
BASE COUNT         1203 a          965 c          849 g          980 t
ORIGIN      
        1 atttcgtaaa aaaaatgccg gcatcgaggt gcgtagtttt acctcagaaa aaaatcacac
       61 gctcccgcag ctcgtgaacc aaaaggcggc acgcctttcg agcgaataca cacaggtgcg
      121 ccttatcgaa gaacgggatt caaaattcaa agaaaacttg cacctctccg tttgggaaat
      181 ccgaacacgg aacaaagtct tgcgcgaaga gactgccatc gtgatggcga acgaaggccc
      241 cgttatagtt tcatgcctta tccccaaagc cgacgaacaa caatacagaa ccatttgcga
      301 taacgcgttc tattccgtcg tgctcgacgc ttcgggcgaa gctgtaatgc ctgcgcagaa
      361 aacagcaaaa tcgtcggttg tagatttttt aacaagactc tattttttgc acgtccccaa
      421 taatttaccc agtataccgc ctgaaactat ttttgatcca ccctctcaac aaaacactgt
      481 ggtgccacct agacccattc aatacgatgg aaactatatt ctgcccaatg ataatccgaa
      541 ataaaacatt acccctactt gtgtttgtcg ctttaacgct atcgccgcaa aacgcaaaaa
      601 tgccatacca atcgacgttg cccgaggcgt tggcaaacac cacaccagcg ctcgaagaaa
      661 tttggggcga agatagctgc gaaactgtta tcgtcaaaaa aaaaggatat ccttttgtat
      721 cgattgcctg ccgtggcaac tatacaaaag ccatgcgcac tcgttttgtc gacacgttac
      781 tcaagaattt ttttaccatc aagcgcgaaa cgtataagct taaaccgcaa aatggtttat
      841 acctctacaa aactcaatac ggcaacgaaa ttgtttacat aaaattattc gtgcgcgagg
      901 cgttcgatct atggcccaaa acccccgtcg acggaaagca aattgcgctc tatatacaaa
      961 acgtgcgcga gagccgcgac cttgtgaagt ggcgcacgct gggtgttccg ttgacgttcg
     1021 gcgtcactgt agggcgcggt aattctagag atctcatcgg acaaatttca tcgtacggcg
     1081 acgaggtgtg gctcgcagtg cctttggaag acgagacgat tgacgttgcc gacggccgcc
     1141 tactcaccat caccgaagca ttggacgaga gtaaaatcgg cgagtacttt aacgagcttg
     1201 cattagaaac ctccgtcacc ggaattagtc cgctctattg ttcgcgcttc tgtaacaata
     1261 taccggcgtt acgcgcgctc tttggcgccg tccaagcgtt gaacgaatct caaaagttag
     1321 tggtgttaga cacaatgccg acgcctaacg cgtcgccttt ttatcaaact gcaaggatta
     1381 tgaatttcag agcgttccgc gctcatcctt ttttaagcgg tgaaccgcaa gcgtgcaccc
     1441 acttacaaaa ttttgccgcc gaaggaggcg aaaacgccac gcgtattatt gccatcgatg
     1501 caggcgatga aaacgcatat aattgtttaa gccgcatcgc ccaaaccaat acccgcactg
     1561 ttgattttgt aaaaatcagc gcgctttcat taacgaatcg atttcgctaa taaagccgcg
     1621 tcgagggtta atccccctga atcatttttt ttaagagtga gaacgtaacc gtcgggtaag
     1681 cggtggagaa attgttcgag aggcaacgtt tcagtagtct gcgcgtacga aattgtcgct
     1741 gcctcttcac cgatacggag cgtcaccacc gaaccgaaac cgtagagctc acagccaaac
     1801 gggtttttag acgccgtgag gaacaccgta gaattttttt cgatggcgac tgcggcattt
     1861 gtttgcttcg ccacactttt tctccgttgt tccaaaaccg attcaattct tcgtcggatt
     1921 aacttaaatg aataataaaa aagtagtaac gcaaaaacaa caccgattaa agaaaagacg
     1981 aaagcgttgc ggtactccga tggatttttg agcgcatacg taagttctgc aacccaacta
     2041 tgcggtcgaa cgacaagcgg tacgcgctga agctcaagcg agcccgcaag aaggcgcact
     2101 gttccacgga aatacgcgtc gggtaacgcg cgcacggcaa cgataaactc cgcgtgcgtg
     2161 ttttcaaact gcggtaaaag aaagcgcgcg cttttttttc gacaccgctt cgaggtgcaa
     2221 gtgatgcgct caccctgaaa gaaaaattcg gtttgggttt cgttcacctc gggtataaat
     2281 tccaactcga gcggagcgta aatgggttta tccgcattga gaacaaaacg aatcggcacc
     2341 gcttcgttcc aaaaagaaaa gcgactcgcc tccacgctcc actgaatatc tgttttactg
     2401 atatccactt caaaaatagc gtttgctttg ggtgtgttga tacagatcag atgcccaaga
     2461 aaagctaact gtacatcggt tttgaaattc aaccgaaatt cttttttact caaaaaaatc
     2521 ggcgcttttt gcgagggcat cacacgccca ctttcatgca cgcacgtgaa gtaactccaa
     2581 tcgcctgctt tcggcacatt gggtctcgaa tctagataat gcaacgagaa acgccccgcg
     2641 tacgcgtggt gggtaaactc ggcgtatttg cgccacaaat cgcttcggtc aaaaaaacct
     2701 ttagggagtt gtacactttc ttgggcaaat gcatttttta ccaagaaagt aatgttttcc
     2761 gttttttgtt ggtatgcttt atctttcgca agtttatcga tataagcgtc tttaggccac
     2821 tcgtcgctac cgatataaat agcgtggtcg ccttttttaa gcctttctgc aaacgcaacc
     2881 gcgcgttcct ctcgtaagaa atccttcacg taatcgagtt cgacttcttg cgcgctcgtc
     2941 aaaggtacaa acttgtagat gacttgttca actttattat tacgtaattc ttcatcgagc
     3001 agcaaataga gtttcgcttc tattcgattg agcgagaaaa agaaaataaa aaatacgata
     3061 tattttttaa acaacttgct tcgtcttttt ttctgcaaag agtcgggttg ccgccacaac
     3121 acaaagcaac ggagccaaga taacgccaac gacagggata aagacaaaaa ctgaagagaa
     3181 gactcccaac cccgtcgctt ctgctttatt gccttgaatc atgccgctaa actcgcgcgg
     3241 acgccccact aaatcaaaaa ccaaatcaaa atacgagcgt ccgataaaat agctggaaga
     3301 gaaaaaattc aaaaacggtt gtaatggtcc cgtaaaaagg agcagcaatg caacgccccc
     3361 ttgcaacaaa agcattttga tactgtacca taccgttgaa acgatatacc caaaaatctc
     3421 agacgccttt attggtaacg tatcgatacc ttcggcgtgc aaaatttttt caatgacagg
     3481 cgacaaaaag ggaatcacaa tgactgagaa aagaactaag taaacacgaa gcataaacga
     3541 aaaaagaaaa tagaaaaaaa ccaatttgaa aataaattca aaaaggtaat aaaaccatcg
     3601 taagaggaaa aaccaattta ccgtatcgac ctgaagccac gcagtaatcg agctcaataa
     3661 aaaaaaccaa aataaaaacc ctagcagagc cgctacaccc gccgtcaaaa gtagtggaaa
     3721 cacggtgtat ccccacaact ttttttctgt aatgtacgat atactgttga gaggcaaact
     3781 tatgccacgc ccaatgcgtt caaacattgt ttagaagaaa gaaaagcagc gttgccgtaa
     3841 agacaatttt ttgatttcgt tatcgtgcgc caaaaattgc gctgccgatg cgcaggtgcg
     3901 ttgcgccttc ttggatggcg agcttgaagt cgctcgacat acccatagaa agtatattct
     3961 cgtaagtcga aaaaattttc tgcgccgcga tgttttc
//
LOCUS       DAOMPD010000244         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9433_length_3978_cov_2.193219, whole genome shotgun sequence.
ACCESSION   DAOMPD010000244 DAOMPD010000000
VERSION     DAOMPD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9433_length_3978_cov_2.193219"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLY93_08995"
     CDS             complement(<1..993)
                     /locus_tag="PLY93_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP69653.1"
                     /translation="MKLTSTILISLPLLMGGFVQFIISQFYQKFATDALLLESAVVGS
                     IFFISRVSDAVLDPVCGYLSDRLRSRRAFIFLGLIALALGTYTAFLPALFETAPLALR
                     YAFAACGIFILYLGVTLVYIPHYAWLAALYRTTKAPLFAARAVIENFGTILGGAALAL
                     LVPYQTSVTGKGGSLLTTLGIMLVGPLLLGIFPLLLYKSTVDDTPSIPSFFHSMKTLL
                     KNKRFTFVSLMSFFNQFAATTLLAVSLYYSDYVLHNKELGVTMTIVFLLSATIFVPLW
                     SYLTRHFSRYTLWSLALLIIVAMSPTLLLAENGFAQYVIVFAFVAGAAAGAVLLF"
     gene            complement(993..2516)
                     /locus_tag="PLY93_09000"
     CDS             complement(993..2516)
                     /locus_tag="PLY93_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69654.1"
                     /translation="MRRALYGFILLFILVNCKKATLHFNDKNTAVANALFLETDRNVS
                     GIFTAKASSFFVRIKITEPVMLRAEVSAIANGDVSLALFHPADTLLFEVNDNGVGNAE
                     EISPVYLHTGEAIIRISNQGKHDAPFTFFYRTFNPPSNIEREPNNNFTTATKIEENRA
                     TGFYGPLYFKRGDIREKEQDCFVAQNTEPQNKNLSVQLSKVVGVRGSLNIFDEHLNSI
                     YTTTQEEKETFLKTPPVRILGERVYVCVAAEETKPTRSRDYYDLTFTWAPSSNDTEQE
                     PNDNPTAANTVIGEKIEATIHSLSDVDYFVYENHREYAVSIRVELKSIPENTLSFLRI
                     INNKEVTVFEGNAPRSEIAENLSIDANEKIILYVKNNKRTTKKNFKPSTYTLHFQETA
                     LTDENEVEPNNTPTTADTLVDLTQKTGFINPVGDVDFYRLKFDAPHDRILTFESKIDC
                     LLQLTHLRQNKTIKKQTGKERLLYNANFHNDDLIKLHCTNQKSFPKERAYRVQLIEP"
     gene            complement(2519..2821)
                     /locus_tag="PLY93_09005"
     CDS             complement(2519..2821)
                     /locus_tag="PLY93_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69655.1"
                     /translation="MESPAIAITNNVTPGANPPEYVFDLIQISLLAASGDIRALSKAV
                     TTARSNGADNRQILRAMFRSDEAFHAGIYSNEFLHLASSLQAGYSPLPKEAHIKSA"
     gene            complement(2928..3692)
                     /locus_tag="PLY93_09010"
     CDS             complement(2928..3692)
                     /locus_tag="PLY93_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP69656.1"
                     /translation="MVHLHYDYYAGDKSRTVIFLHGLFGSAKNFTTLAESITDVAETY
                     ALDARNHGRSPHTETHNLDDLVLDVKNFIGERSIKSPILIGHSMGGLTAMAYASENPV
                     RGLVILDIAPRSYAPGHMQEIVAQKMDLSAFKTRQEIDTKMAEILPNKTVRQFLQMNI
                     NRVDEHFIWTNNIAAIERSQERTQFPNFKFPLYIGPVLGVRGLQSDYVTDTDVQLMHK
                     AFPQLTMHDFADATHWLHHTHRAVLLQIIRSFLVKL"
BASE COUNT         1028 a          796 c         1011 g         1143 t
ORIGIN      
        1 aaaaagaagc acagctcctg ctgcggcacc cgccacgaaa gcaaaaacaa taacgtactg
       61 cgcgaaaccg ttttcggcca aaaggagggt gggggacatg gcgacaatga tgagtaaagc
      121 cagcgaccaa agtgtgtagc gcgaaaaatg gcgcgtaaga tacgaccaca gcggcacaaa
      181 tatggttgcg ctcaacagaa acacaatcgt catcgtgacg ccgagttctt tgttatgcaa
      241 aacataatcg ctgtagtaaa gagaaacagc taacaacgtc gtcgcggcaa attggttaaa
      301 aaagctcatg agcgaaacga aagtgaagcg tttatttttc aagagagtct tcatcgaatg
      361 aaaaaaagag ggaatggagg gagtatcgtc gactgtgctt ttgtagagaa gaagtggaaa
      421 tatccccaac agcaaaggtc cgacaagcat aatgccgagc gtcgttaaaa gacttccacc
      481 tttacctgta accgacgttt ggtagggcac taaaagcgcg agtgcagcac cgcccaaaat
      541 ggtaccgaaa ttttctatca cagcacgtgc cgcaaaaagc ggggctttcg ttgtgcggta
      601 aagagccgcc agccacgcgt aatgtgggat atagacgagt gtgactccta aataaagaat
      661 gaaaatacca caggcggcaa acgcgtagcg caacgcaagg ggtgcggttt caaaaagcgc
      721 agggagaaac gccgtgtacg tgcccagcgc gagagcgatg agtcctaaaa aaataaacgc
      781 gcggcgcgag cggagtcgat cgcttaagta accgcacacg ggatctaaca cggcgtcact
      841 cacgcggctg ataaaaaaaa tagagccgac gactgcgctt tctaaaagga gcgcgtcggt
      901 ggcgaatttt tggtagaatt gtgaaatgat gaattgcaca aagccgccca tgagtagcgg
      961 caaagatata aggattgtag aagttagttt cattatggtt caatcagttg tactcggtag
     1021 gcgcgttctt tagggaagga cttttggttc gtgcagtgga gtttaattaa atcgtcgttg
     1081 tggaaattag cgttgtagag taagcgttct ttacctgttt gcttttttat tgttttgttt
     1141 tggcgcaagt gggtgagttg taataagcaa tctattttgc tttcaaaagt caaaatgcgg
     1201 tcgtgtgggg catcgaattt taatcgataa aagtccacgt cgccgacagg gttgatgaat
     1261 cctgtctttt gcgttaagtc gactagtgtg tcggcggtcg tgggggtgtt gttgggttcg
     1321 acttcgtttt cgtcggtgag cgcggtttct tggaagtgta gcgtataggt agagggctta
     1381 aaattttttt tggtcgtgcg tttattgttt tttacgtaca atataatttt ttcatttgcg
     1441 tcaatcgaca agttttctgc gatttcgctt cgcggcgcat taccttcgaa cacagttact
     1501 tctttattgt tgataatgcg taagaagctg agcgtatttt cgggtatgct ttttagttcg
     1561 acgcgtatcg acactgcgta ttcgcggtgg ttctcgtaga caaaataatc gacgtcgctt
     1621 aaggagtgta tcgtcgcttc gattttttca cctatgactg tgtttgctgc tgttggattg
     1681 tcgttgggct cttgctcggt gtcgtttgat gaaggcgccc atgtgaatgt aaggtcgtag
     1741 taatcgcggc tacgcgtagg tttcgtttct tccgccgcca cgcacacgta aacgcgctcg
     1801 cctaaaatac gtactggcgg cgttttgagg aacgtttcct tttcttcttg agtggttgtg
     1861 taaattgaat tcagatgttc gtcaaaaatg ttgagtgagc ccctaactcc aacgactttg
     1921 cttagttgca ccgataaatt tttattttgg ggttctgtat tttgtgcgac aaagcagtct
     1981 tgttcttttt cgcggatgtc tcctcgtttg aaataaagcg gcccgtaaaa gccagttgct
     2041 cgattttctt ctatttttgt cgccgtggta aaattgttat tcggttcgcg ctcaatgttg
     2101 gagggtggat tgaaggtgcg ataaaaaaaa gtaaacggtg catcgtgctt cccttggttg
     2161 ctgatgcgga ttatcgcctc acctgtgtgt agataaaccg gtgaaatttc ttcagcgttg
     2221 ccgactccat tgtcattcac ttcaaaaagt agtgtatcgg cgggatggaa aagcgccaac
     2281 gagacatccc cgtttgcgat tgcggaaact tcggcgcgga gcatcacagg ttcggtgatt
     2341 tttatacgca caaagaaact cgatgccttt gcagtgaaaa tcccactgac gttccggtct
     2401 gtctctaaaa aaagcgcatt ggcaacagcg gtgtttttgt cgttaaagtg tagcgtcgct
     2461 tttttacaat ttactaaaat aaataagagg atgaaaccat aaagcgcacg gcgcataact
     2521 atgcagattt aatgtgcgct tctttcggta aaggtgaata ccctgcctgg agcgatgacg
     2581 ctaagtgtaa gaattcgttt gaataaatgc ccgcatgaaa cgcttcgtcg ctgcggaaca
     2641 tggcgcgcag aatttggcgg ttgtcggcac cgttgctgcg ggcggtggtc accgctttcg
     2701 ataatgcacg aatgtcgccc gatgcggcta acaaagaaat ttggattaaa tcaaacacat
     2761 attcaggtgg attggctccc ggtgtaacat tgtttgtaat agcaattgct ggggattcca
     2821 taattatata ttttgtgcgt tgcacaaagc gtcaattaaa aatcttgtcc gatacacttt
     2881 tcgtcctttc attaacgagc ctgtgcgcca actacgctgg aggatagtca taatttaact
     2941 aaaaaggatc gtataatttg tagcagcact gcgcggtgcg tatgatgcag ccaatgcgtt
     3001 gcatccgcaa aatcgtgcat cgtcagttgt gggaatgcct tatgcatcag ttgaacatcc
     3061 gtatcggtga cgtaatccga ctgaaggccg cgcactccga gcacaggacc gatataaaga
     3121 gggaatttaa aattgggaaa ttgtgttcgc tcttgtgagc gttcaatcgc cgcaatgtta
     3181 tttgtccaaa taaaatgttc atctacccta ttgatgttca tctgcaaaaa ttgccgtact
     3241 gtcttgttgg gtaaaatttc agccattttt gtgtcgattt cttggcgcgt tttgaatgcg
     3301 gataagtcca tcttttgtgc gacaatttct tgcatgtgtc cgggggcata actacgtgga
     3361 gcgatgtcga ggataactaa cccacggaca ggattctcgc ttgcgtacgc catcgctgtc
     3421 aatccaccca ttgagtgccc tatcaaaata ggacttttta tactacgctc cccgataaaa
     3481 ttttttacat cgagcactaa gtcgtccaag ttgtgtgttt cagtatgcgg cgagcggccg
     3541 tggttgcgcg catcgagtgc ataggtttcc gctacatcgg tgatggattc ggcgagcgtc
     3601 gtaaaatttt ttgccgagcc aaaaagaccg tgcaagaaaa ttacagtgcg gcttttgtcg
     3661 cccgcgtaat aatcgtagtg taggtgaacc attgggggaa gttttccatg tccgttttgc
     3721 gtgtcaacgt ggttcgatat gggtaaggtt gtggatttcc caaaagttcc ttacgtcttt
     3781 tttctgtaag gggttagggg gattgtttat tataaaaata tacgggataa ctagtctcgc
     3841 ctggttcgag tgtggattat gtcatattta gggataacta ttctaaccga caattttcct
     3901 caaaaaaaat ctaatttttt ggaaaaattt gcactttttt ttccaaaagt gagaaaattt
     3961 gaccgctttt tggtattt
//
LOCUS       DAOMPD010000245         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9435_length_3977_cov_2.755227, whole genome shotgun sequence.
ACCESSION   DAOMPD010000245 DAOMPD010000000
VERSION     DAOMPD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_3977_cov_2.755227"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1098
                     /gene="metF"
                     /locus_tag="PLY93_09015"
     CDS             193..1098
                     /gene="metF"
                     /locus_tag="PLY93_09015"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRP69657.1"
                     /translation="MHSPRLIADILREQKPALSFEFFPPKTQEGADTLFQTISELKSL
                     SPAYVSVTYGAGGSTRGLTHDLVVRLSQELKFTIVSHLTCVGSSKEEIHRILERYNEN
                     GIHNILALRGDAPKSDNGEWRPHPNGFHYATEMVAFIKKHFPQMGIGVAGFPEGHPET
                     PNRLKEIEYLKAKVDAGADYICTQLFFDNHDFYDYRERCALAGIHVPIVAGIMPITSR
                     KGMQRMAELSLGSRFPARLLRALARAEDDAYAENVGIHWATEQVLDLIDNGVAGVHFY
                     TLNKSKATLKIYDSLGIKRSYGIQH"
     gene            1082..2575
                     /locus_tag="PLY93_09020"
     CDS             1082..2575
                     /locus_tag="PLY93_09020"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRP69658.1"
                     /translation="MEYSIEFYILPISLLFLFSLVAGKISSRLGLPALLLFLAVGMIS
                     GSDGLGIQFHDANTAHAVSTVALCVILFSGGLDTKLDEIKPIAAAGAVLATVGVFLMA
                     AITGFLTWWILKNDAAVAAGISVFTALLVASIMSSTDSASVFSILRSRKLYLKNNLRP
                     LLELESGSNDPMAYLLVITLVSLMKNGADVSFANAALLLLIQLVVGAAVGYLLGKGAV
                     HFINRIGMTNAALYPILVFTIALLVFSVSYFIRGNGYLAVYVAGFIIGNSKFQHKRSS
                     LEFFDGLAWLSQLLTLGLLVNPHELLPVAGPAVVISLLMIFIARPASVFLCLLPFKRF
                     DFRDKVFISWVGLRGAVPIIFAIIPLAENVLHARLIFNIVFFCTLASLLIQGTSLSLV
                     AKWLKRVDENASEANPPMASVFAEEIKSVSSEIQITAESLIAGKRLMDLPFPEMAHVV
                     VVRRKDCYFVPTGASELIEGDVLLVISNDSALLKNREIGKMLPEQHG"
     gene            complement(2602..3390)
                     /gene="thiD"
                     /locus_tag="PLY93_09025"
     CDS             complement(2602..3390)
                     /gene="thiD"
                     /locus_tag="PLY93_09025"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRP69659.1"
                     /translation="MTSVALTIAGSDSGGGAGIQADLKTMAALGVFGTSAITAITAQN
                     TCGVDAVEILSSQIVYAQIQSVVSDFYLGAVKTGMLGNTILIETVAKAIHDFNIEHLV
                     VDTVMVSKSGHRLLAEDAEEAMRKTLLPYAYIVTPNIPEAEVLLGKNISTLAAMKNAA
                     EALHEMGAKYVLVKGGHLTESADDVFFDGFEFTVLEGERIDTTSTHGTGCTLSAAIAA
                     HLALGASPLEACRLAKEYLTGALRHAVPQGRGHGPVSHFWQHRF"
     gene            complement(3387..>3977)
                     /locus_tag="PLY93_09030"
     CDS             complement(3387..>3977)
                     /locus_tag="PLY93_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRP69660.1"
                     /translation="NSFTYSIPPDNPFATSTGEAGKPKREIFAWGLRNPWRFSFTPEN
                     KLIVADVGQDMYEEISIVERGANMGWNTVEGFHCFKPKKNCNQSGLTPPIYEYDHGVG
                     QSILGGYVYEGSAMTQYKGRYIFADSVSGRVFALPYREKNPKAEELIQAPGLWSSFGR
                     LRNGELVISELQSGRIFMLTPAKQVGAQDVLKGGIR"
BASE COUNT          909 a         1028 c          964 g         1076 t
ORIGIN      
        1 acatatttgc ctctatatat taaataccag aaaatggtca aattttctca cttttgcaaa
       61 aaaaaagtgt aattttttag aaaaaatcac ttgtgtggat agttatcctt cacgcgagca
      121 gaacgactca ccctcaataa cgaacatacc ctttcttctt tacggcacgt taatagagaa
      181 ccgatacgaa cgatgcactc gcctcgcctt atcgccgaca ttttgcgcga acaaaagccc
      241 gcgctcagtt ttgaattttt cccgcccaaa acacaggaag gagccgacac tcttttccag
      301 accatcagcg agcttaaaag tttatcccct gcgtatgtga gcgtcactta tggcgcgggt
      361 ggatcaacgc gcgggttgac gcacgacctt gtggtgcgtt taagtcaaga actcaagttc
      421 acaattgttt cgcacctgac ctgcgtgggt tcatcgaaag aagaaatcca ccgcatcctg
      481 gagcgttaca acgaaaatgg tatccacaat attttagcgt tacgtggcga tgctcccaaa
      541 agcgataacg gcgagtggcg gccacacccc aatggttttc attacgctac agaaatggtc
      601 gcgtttatta aaaaacattt cccgcagatg ggcattggag tggctggttt ccccgaaggc
      661 catcccgaaa caccgaaccg ccttaaagaa attgaatatt tgaaagcaaa ggtcgatgca
      721 ggcgccgatt atatctgcac tcaacttttt tttgacaacc acgatttcta cgattatcgc
      781 gagcggtgcg cattggcggg catccatgtg ccgattgttg cgggtattat gcccatcacc
      841 tcgcgcaaag gcatgcaacg tatggcagag ctttcgttgg gttcgcgttt ccccgcgcga
      901 cttttgcggg cactggcgcg cgccgaagac gacgcgtacg cggagaacgt tggcatacac
      961 tgggcgaccg agcaggtgct cgacctcatc gacaatggtg tcgcgggcgt gcatttttat
     1021 acactcaaca aatcaaaagc gacgctaaaa atttacgatt cactcggcat caagaggtct
     1081 tatggaatac agcattgaat tttatatcct acccatctct ttactttttt tatttagtct
     1141 cgtcgctggt aaaataagtt cgcgcttggg attacccgcg ttactgcttt ttttggctgt
     1201 ggggatgatt agcggcagtg acggtttggg aatccagttc cacgacgcga acactgcgca
     1261 tgcggtgagt acggtggcgt tgtgcgtgat tttattttca ggcggattag acaccaagct
     1321 cgatgaaata aagcccatcg ccgccgcagg cgccgtgctt gcgaccgtcg gtgttttttt
     1381 aatggctgcg attaccggtt ttctcacttg gtggatctta aaaaacgatg cggctgtcgc
     1441 agcggggatt agcgttttta ccgcgctctt ggtggcgtcg attatgtcgt cgaccgactc
     1501 ggcatcggtg ttttcaattt tacgttcgcg caaactctat ttgaaaaaca acctccgtcc
     1561 gcttttagaa ttagagagtg gcagcaacga ccctatggcg tacttgttgg tgattacatt
     1621 ggtgtcgctc atgaaaaacg gcgcggacgt tagtttcgcg aatgcagcgc tgctcctttt
     1681 gattcaactt gtcgtcggcg cggcggtggg ctatctttta gggaaaggcg ccgtgcattt
     1741 tatcaaccgc atcggtatga caaacgccgc actttatccc attttggttt tcaccatcgc
     1801 gttactcgtg ttttcggttt cctacttcat ccgtggaaac ggttacttgg cggtctacgt
     1861 cgcgggtttc atcatcggca attctaaatt ccaacacaaa cgctcgtcgc ttgagttttt
     1921 tgacggctta gcgtggttga gccagctttt gacgttggga ctccttgtca acccgcacga
     1981 gttgctgccc gttgccggtc cagcggttgt cattagcctg ctgatgattt tcatcgcacg
     2041 ccctgcgtcc gtttttttat gcctactgcc tttcaaacgg tttgatttcc gcgataaagt
     2101 ttttatttca tgggtggggc tgcgcggcgc ggtaccgatt atttttgcga ttatccctct
     2161 tgcagaaaat gtgttacacg cacggctcat tttcaatatc gttttctttt gcaccctcgc
     2221 atcgctcctc atccaaggca cgtctctcag tcttgtagcc aaatggctta aacgcgtcga
     2281 cgaaaacgcc agcgaggcca atccccccat ggcgagtgtc ttcgccgagg agataaagtc
     2341 ggtgtcgtcg gaaatccaaa tcaccgccga gtcgctcatc gccggtaagc gtctcatgga
     2401 tttacccttc cctgaaatgg cgcacgttgt ggtggtgcgt cgtaaggatt gttattttgt
     2461 acccaccggc gccagcgaac ttatagaggg ggatgtactc cttgttattt ctaacgacag
     2521 cgcgcttttg aaaaaccgcg aaatcggtaa aatgctgccg gaacaacacg gttaactttc
     2581 cagacctatt aataaagtta ctcagaagcg gtgctgccaa aaatgcgata ccggaccgtg
     2641 cccacgcccc tgcggcaccg catgtcgtaa cgcgccagtt aagtattctt tagcaaggcg
     2701 gcacgcttct aaaggcgatg cccccagtgc caggtgcgct gcaatcgctg ccgaaagagt
     2761 gcatccagtg ccgtgcgttg aggtggtgtc gatacgttcg ccttccaaaa cggtaaattc
     2821 aaatccatcg aaaaaaacat cgtcggcact ttcggtgagg tgcccccctt tcaccaacac
     2881 gtattttgcg cccatctcgt ggagtgcttc ggcggcattt ttcatcgcgg caagcgtcga
     2941 gatatttttt cccaaaagta cctccgcttc ggggatgttg ggggtaacta tgtacgcgta
     3001 gggcaagagt gttttacgca tcgcttcttc ggcgtcctcg gcgagtaagc ggtgcccact
     3061 tttagagacc atcaccgtat cgacaaccaa gtgttcgata ttaaaatcgt ggattgcttt
     3121 tgcgactgtc tcgataagaa tagtattgcc gagcatcccc gtttttactg ctccgagata
     3181 aaaatcgctc actacacttt ggatttgtgc gtacacaatt tgagaggaga gtatttccac
     3241 tgcatcgacc ccgcacgtgt tttgtgcggt aatcgccgta atcgcactcg taccgaaaac
     3301 gccgagcgcg gccatcgttt ttaagtcggc ttggataccc gcaccgccgc cagagtcgct
     3361 gcctgcgata gtgagcgcga ccgacgtcaa cggattcctc ctttcaagac atcttgcgca
     3421 cccacttgtt ttgcgggagt aagcataaaa attctccccg actgcaattc gctaatcacc
     3481 agttcgccgt tgcgcaaacg accaaagctt gaccaaagtc ctggggcttg gataagctcc
     3541 tcggctttgg ggtttttctc acggtaaggc aatgcaaaaa cacgtcccga taccgaatcg
     3601 gcaaaaatat accgtccctt atactgcgtc attgcactcc cctcgtacac gtagccgcct
     3661 aaaatcgatt gccctacacc gtgatcgtat tcataaatgg ggggcgttaa tccgctttgg
     3721 ttacaatttt tctttggctt gaaacaatga aaaccttcca ccgtgttcca ccccatgttg
     3781 gcgccacgct cgacgatgct aatttcttcg tacatatctt gccccacgtc ggcgacaatg
     3841 agtttgtttt cgggtgtgaa actaaaacgc catgggttgc gcaatcccca cgcaaaaatt
     3901 tcgcgtttgg gcttgcctgc ttctcctgtc gaggtagcga atggattgtc cggtgggatt
     3961 gagtacgtga atgaatt
//
LOCUS       DAOMPD010000246         3970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9450_length_3970_cov_2.825543, whole genome shotgun sequence.
ACCESSION   DAOMPD010000246 DAOMPD010000000
VERSION     DAOMPD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9450_length_3970_cov_2.825543"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLY93_09035"
     CDS             complement(<1..677)
                     /locus_tag="PLY93_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69661.1"
                     /translation="MKATQTPPSNQPLVEYEDFSFVAGKIITSTKHIYGRLKTIRAHT
                     HLECITVEYERINKIEALCEEIDKKKRYAVSFEAQYIIYPTKKKSPLSLRTGTTRIRY
                     WNFSESDFFKLNFLRSVHDKKLFLAHFSQSDLLFFIAAAFFHSEPRLSEAAYKQAVKY
                     LSKENFSENDARAFLFYIRKILKGDAPKIATIIHTFQKVDTAALFTVYLLKLLARYSY
                     FDLSLQK"
     gene            complement(649..2403)
                     /locus_tag="PLY93_09040"
     CDS             complement(649..2403)
                     /locus_tag="PLY93_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69662.1"
                     /translation="MTFFFVVTFILFFTTYLGAASTWPISGLTIIDTQWNSWVTRFFA
                     MDDKNNLYYGRVVDQENKLMKVHRFYRKPDLVKHFPMDDGDLVVSIYNNEKIMQFVAT
                     DFTGEVRYANEFALSFNVYDVDARVNIQEGNPSCLFYAYDKRTYSIKYWSELRTQNIL
                     VSRDPIELMLLQWNDGGVHYVSVSSSKMVWTFWKNGEYRTFPLPFAIKNARFYKYRNT
                     IHLIGIDHQGGLWQFDIAAGMLRRNLLEKDKRLVGVERVTAFNFRKELNILLTGEHIS
                     SAYRLTFDDFPIPRKKPKLEERKLFWEGKVFPLIDNTNQLNFLLETTLRHIYLETWST
                     PTAIITDIDWRLDLKMNPPSMIVHWVRPKGSEYAYRYLLDQNPDSEPLNEAKLIPTDT
                     LRFSARKEGDYVLHLQVRNVRSGALSRMYHIPLEWQYQPPEPDVLLLNQVAPRMVNPG
                     KVNFVLKQLEPGTYYAALDNKSDTIPKKIADVSTGGLSVNVRRPGRYYLHLANRDERS
                     QVLSPTMHYLFFVTPFDVDEDPTLSETRRLIDEIQRIKKKIEAAKGEPAKTQPWINRL
                     EDIEKQMKKNESDPNTTQ"
     gene            complement(2460..>3970)
                     /locus_tag="PLY93_09045"
     CDS             complement(2460..>3970)
                     /locus_tag="PLY93_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69663.1"
                     /translation="DHLVTKEGISKKPLEFARWGERPFLFYGCGPGLCARETDTLASP
                     IKVNSKTHALGMVSASSDAIFTWNKITDLSGIESYAWTIDDQKDTDPDLYNLNARALQ
                     ITMPGLNGGAYFMHIKYRDRAGNVSPITHYPFVVDSIPPSRPIIKSPSHANGIPDSRR
                     DVIFKFISSDDSGIQFYRYAFGEVLPRTFTETTEKGELTFEEVPYGSYYFAVEAVDLG
                     GNVSERGYYKIEIGANERNDLSIRHNAEADVITRPDIAFTIQDNGARGIKEVYYQFGF
                     DIKDPFAGQKAALEALDNIYTARIRGLEKGISVISLGIVYNDGSHAAARHFYFDSNDP
                     RARKKFAFVDVEYEKLPLVKPRAPFQVGGRADLISSVFDRGILEIRLNYDPTTICGIV
                     STSASKKPCDAARKVKVKGYVWAQGEINFSGKVEFIYLQKPGNYFLNAKTIFRGPERE
                     QFAYDSKKIEAPDLRKAFRKNFYLGVGALLCVLLLFAFWQRKRIIFYTGAWV"
BASE COUNT         1117 a          918 c          807 g         1128 t
ORIGIN      
        1 attttttgta acgataaatc gaaataacta taccgcgcta ataatttcaa aagataaact
       61 gtaaaaagag cggcggtatc cactttttga aacgtatgaa tgattgtagc gattttgggg
      121 gcatcgcctt ttaaaatttt tcttatataa aaaagaaatg cccgcgcgtc gttctctgaa
      181 aaattctctt tcgagagata ttttactgcc tgtttatacg ctgcctcgga taatctgggc
      241 tcgctgtgga aaaatgcggc ggcaataaaa aaaagtaaat cgctttgaga aaaatgggcg
      301 agaaataatt ttttatcgtg cacgctcctt agaaaattta gcttaaaaaa atctgattcg
      361 ctgaaattcc aataacgaat gcgcgtcgtt ccagttcgta aagacaaagg agattttttc
      421 tttgtcggat aaattatgta ctgcgcttca aacgaaaccg catagcgttt ttttttatcg
      481 atctcctcgc acagcgcttc aattttgtta atacgttcgt attcgacagt gatacactcc
      541 aagtgggtat gagctcgaat cgtttttaat cgaccataaa tgtgttttgt cgaagtaatt
      601 atttttcctg caacaaacga aaaatcttcg tactcgacca aaggttgatt actgggtggt
      661 gtttgggtcg ctttcatttt ttttcatctg tttttcaatg tcttctaagc ggttaatcca
      721 cggttgagtt tttgccggtt cgccttttgc cgcttcaatt tttttcttaa tgcgctgtat
      781 ctcgtcaatg aggcggcgtg tctccgacag tgttgggtct tcgtcaacat caaaaggagt
      841 aacaaaaaat agatagtgca tcgttgggct caaaacctga ctgcgctcgt cgcggttggc
      901 aagatgtaaa taatagcggc caggtctacg cacgttgacg ctcaagccgc ccgtcgaaac
      961 atcggcaatt tttttgggga ttgtatcgga tttattgtcg agcgcagcgt aatacgtgcc
     1021 gggttctaat tgcttaagca caaaattaac ttttcccgga ttcaccatgc gcggcgcaac
     1081 ttggttcaga agaagtacat ctggttcagg gggttgatat tgccactcta aaggaatatg
     1141 atacatccgc gacagagcac cgctgcgcac gttacgcact tgtaaatgca atacataatc
     1201 accctccttg cgcgccgaaa aacgtagcgt atcggtgggt ataagcttgg cttcattcaa
     1261 aggttcgcta tcgggatttt gatcgagcaa gtaccggtat gcgtactcac tccctttggg
     1321 gcgcacccag tgcacaatca tcgacggtgg attcattttc aaatccaacc tccaatcgat
     1381 gtctgtaata atcgcggtgg gagtgctcca cgtttctaaa taaatatggc ggagcgtcgt
     1441 ctcgagtaaa aaattgagct ggttggtgtt gtcaatgagc ggaaaaacct ttccttccca
     1501 aaataatttc ctctcctcca gttttggctt tttacgcggt atggggaaat cgtcgaatgt
     1561 taaacgatac gccgatgaaa tatgttcgcc tgtgagtaga atgttcaact ctttacggaa
     1621 attaaacgca gtcacgcgtt caacgccaac aaggcgctta tctttttcaa gtaaattacg
     1681 ccgcaacatt cctgctgcaa tatcgaattg ccataaacca ccttggtggt cgatgccaat
     1741 caaatgaatg gtgttacggt atttgtaaaa gcgcgcattc ttgatagcaa acggtaaagg
     1801 gaacgtacgg tattctccat ttttccaaaa cgtccaaacc atttttgaag acgagacgct
     1861 tacgtaatgc acaccgccgt cgttccattg cagcagcatc aattcaatgg ggtcgcgact
     1921 caccaggatg ttctgcgttc tcagctcgct ccagtattta atcgagtagg tacgtttgtc
     1981 gtacgcgtaa aacaaacacg atggatttcc ttcttgaatg ttcacacgcg cgtcgacatc
     2041 gtacacgtta aaactcaacg caaactcgtt ggcgtaccga acctcgccgg taaaatccgt
     2101 cgcgacaaat tgcataattt tttcgttatt gtaaatgctc acgactaaat cgccatcgtc
     2161 catggggaaa tgttttacga gatcgggttt gcgataaaaa cgatgaacct tcatcagttt
     2221 attttcttga tccaccacgc ggccatagta caaattgttt ttatcgtcca tcgcaaaaaa
     2281 acgagtcacc catgaattcc actgtgtatc gattatcgtt agcccactaa tcggccaagt
     2341 cgacgctgcg cccaaatagg ttgtaaaaaa taaaataaaa gtaacgacga aaaaaaatgt
     2401 catacgaatt tttctcgcga aaaacatttc gacttttatg agtggccgtg tcgatttact
     2461 cacacccacg cccctgtgta aaaaataata cgcttacgtt gccagaaagc gaataacaac
     2521 aatacacaca aaagagcgcc cacacctaaa taaaaatttt tacgaaacgc tttgcgtaaa
     2581 tcgggcgctt ctattttttt tgaatcgtaa gcaaattgtt ctctttcagg cccacgaaaa
     2641 attgtttttg cgttcaaaaa ataattcccg ggtttttgta gataaataaa ctccactttt
     2701 cccgaaaaat tgatttcacc ttgcgcccac acatagcctt tgactttcac cttgcgtgcc
     2761 gcgtcgcacg gcttttttga cgccgatgta cttacaatac cacaaattgt agtggggtcg
     2821 taattgagcc ttatttctaa aataccacga tcaaaaaccg aactaatcaa atctgcacga
     2881 ccgcccactt ggaatggcgc acgcggtttc accaaaggta atttttcata ctccacatcg
     2941 acaaaagcaa attttttacg cgcgcgcgga tcgttagaat caaaataaaa atgccgcgct
     3001 gctgcatgcg aaccgtcgtt atatacaata cccaacgaaa taacactaat tcccttttca
     3061 agtccacgga tgcgtgcggt gtaaatattg tctaacgctt caagcgcagc cttttgtcct
     3121 gcaaaagggt ctttaatatc aaaaccaaat tgataataca cttctttaat tccgcgcgcg
     3181 ccgttatctt gaatcgtgaa cgcaatgtcg gggcgtgtga taacgtccgc ctcggcgtta
     3241 tgccggatag ataaatcatt gcgctcgttg gcgccaattt cgattttata gtaccctctt
     3301 tccgaaacat tgcctcccaa gtcgactgcc tccacagcaa aataataaga accgtaaggt
     3361 acttcttcaa acgtcaactc gcctttttct gttgtctccg taaaagtgcg tggcaatact
     3421 tcaccaaacg cgtatcgata aaattgaata ccgctatcgt ccgaagaaat aaatttgaaa
     3481 ataacatcgc ggcgcgaatc aggaatgcca tttgcatgcg acggactttt aataatcggt
     3541 cgcgacggcg gtatcgaatc gaccacgaaa ggatagtgtg taatcggcga cacattccca
     3601 gcacggtcgc gatactttat gtgcataaaa tacgcgccgc catttaagcc cggcatcgtg
     3661 atttgcaaag cgcgtgcgtt caagttatac aaatccgggt cggtatcttt ttgatcgtcg
     3721 atcgtccacg cataactttc aatgcccgat aaatcggtaa ttttattcca agtaaaaatc
     3781 gcgtcactcg acgctgaaac catccccagt gcatgcgtct ttgaatttac tttaatcggc
     3841 gatgccagcg tatcggtttc acgcgcacaa agccccggac cgcaaccata aaataaaaac
     3901 ggtctctccc cccagcgcgc aaactccaac ggtttttttg agataccctc ttttgttact
     3961 aggtgatcaa
//
LOCUS       DAOMPD010000247         3970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9454_length_3970_cov_2.471520, whole genome shotgun sequence.
ACCESSION   DAOMPD010000247 DAOMPD010000000
VERSION     DAOMPD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9454_length_3970_cov_2.471520"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..828
                     /gene="gmk"
                     /locus_tag="PLY93_09050"
     CDS             268..828
                     /gene="gmk"
                     /locus_tag="PLY93_09050"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRP69664.1"
                     /translation="MVERPVFVVSAPSGGGKNSLINILLKEEPRIRHSISSTTRSKRA
                     NEKDGMNYHFLTKDEFEKRIRENKFLEYARVLDNYYGTEYAEIERIFNASCYPVLDID
                     IQGADALRKKRLPLVTFFIVPPSLEVLEKRLRARASESEEEVLKRLQLARVEIEEKNK
                     FDHVIVNDDLKTASDELIAVVRRHLV"
     gene            825..1808
                     /gene="miaA"
                     /locus_tag="PLY93_09055"
     CDS             825..1808
                     /gene="miaA"
                     /locus_tag="PLY93_09055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRP69665.1"
                     /translation="MSVITKPKLIVITGATAAGKSAFIYTHLRSLPLSIINADSRQVY
                     RGIAIAAASPTAEEELLFPHALYNFLPFDANFSAGEFLRVAEREIQKAHEANKVPLIC
                     GGTYFYIHALFHGLLPSMEIPPSITQEVAALSDTAAYAKLLQLDAKAAEQNHPHNAVR
                     VRRALSLCLAHGGPISALAKVGGIYNDYDIQLFIFDAPRALLKERAITRSQKMLDAGL
                     IREIEQLFSESARARKETAWQKIPALTGIGIREFFEMYEKTEKTPQQLSSQEIHTIKE
                     NIIQNTMRLVKRQQTWFRNAAPKPKDTKIVDPSFEYAHIATLVKEFILPAS"
     gene            1834..2079
                     /gene="hfq"
                     /locus_tag="PLY93_09060"
     CDS             1834..2079
                     /gene="hfq"
                     /locus_tag="PLY93_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRP69666.1"
                     /translation="MNKNNLQDFILNQIRKEKMGITIYLSNGVPIKGRVVSFDNFTIV
                     LEHDHKQTLVYKHAVTTISPEKPIRLNPTDTDESEEN"
     gene            2095..3057
                     /locus_tag="PLY93_09065"
     CDS             2095..3057
                     /locus_tag="PLY93_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69667.1"
                     /translation="MITDFFRRLLQITALAAVVTVVYLDFIFVPKGYTTFLIAPTTTG
                     GIDNQGVFQYAEGTNFVPTLFVPYRFQRYQLETQSKIQEIDVKLPLRYSAYLRLNDLF
                     YVQLKIRIEGFISEKGAYAALKALQYKPTDRDKFIEKQLALVVAEYLLDIKGDEKNLE
                     KTKITLAAFLSPTNKGEIQSRLNRQINDSWFTLTSVELKEIYMPDSALYAAQIRNLDE
                     VATADRAALLSQISKEAELALERKRNLEDITKAEKMGALIHENPYILDYYKIEKIAPR
                     AGNVMLDVTSNARHERPTPADLKDKRGRDGKNENSEGGEIGRAE"
     gene            3008..>3970
                     /locus_tag="PLY93_09070"
     CDS             3008..>3970
                     /locus_tag="PLY93_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRP69668.1"
                     /translation="MAKTKTVKVAKSVGLSKSTKVSKEFVENGKPFVMIMAGGSGTRL
                     WPLSRISRPKQLIQIASSKTLIEEAVDRARLLTTPDRIYIGTNKELAAQIQKIVKLDK
                     KQYLLEPTQRNTAPIIAYFTSFLKANGADDHSGVVILAADHHIEPQEAWANTVKLALS
                     RANERLWCIGIKPTRAETGYGYIEIGIEVAPRMSTISSFREKPDYQTASHYQTTERHF
                     WNSGMFVFSISRMREDLRVYQSQMLKLADECAQSSAALQKIFPKMENISIDYAIMEKT
                     AEMAVVACDIGWNDIGSWSALGDLVAADASGNRVEGHALLQDSTN"
BASE COUNT         1149 a          952 c          923 g          946 t
ORIGIN      
        1 ttttataagc cgtggttgac gcaatgctgt gattcttcat ggagcctgtg cgccaactcg
       61 cccgtgcaga gcaaattgga acaatttgcc gtcggcccaa agagccgccg gccttacgac
      121 gttgcacacc gctttggcgg agaaatttgt gcaaaatagc gtacaaaaat ttgtaaaaaa
      181 attgtctcag cgagttagcg cacaggctct ttcgcttggt cacataataa tgatttacat
      241 agcgttttac ttttatagaa actcaacatg gttgagcggc ctgtctttgt ggtttcagct
      301 ccctcagggg gcggcaaaaa tagcctcatc aatattcttc tgaaagaaga gccccgtatt
      361 cgacattcca tctcctcgac gacgcgctca aagcgggcaa acgaaaaaga tgggatgaat
      421 taccactttc tgacgaaaga cgagtttgaa aaacgcattc gtgaaaataa atttcttgag
      481 tatgcgcgcg ttttggataa ttattacggc accgagtacg ccgaaattga acgcatcttt
      541 aatgcaagtt gttatcccgt gttggacatc gacatccaag gagcggatgc gttgcgcaaa
      601 aaaaggttgc ccctcgtgac tttttttatt gtgccgccgt cgctcgaagt tttggaaaag
      661 cgcttgcgtg cgcgcgcttc cgaaagcgaa gaagaggtgt tgaaacgcct acaactggcg
      721 cgtgtggaaa ttgaagaaaa aaataagttc gatcatgtca ttgtaaacga tgacttaaaa
      781 acagcgtcgg atgaactcat tgcagttgtg cgcaggcact tagtttgagc gtaataacaa
      841 agcccaaact tatcgtcatt accggagcca ctgcagccgg gaaatcggcg tttatctaca
      901 cacacttgcg ctctttaccg ctttccatca ttaacgccga ttcacgccaa gtttaccgtg
      961 gcatcgccat cgcggcagcg tctcccaccg ccgaagaaga gttactcttc ccgcacgcgc
     1021 tctataattt tctgccgttc gatgcaaatt tctctgccgg ggagtttctc cgcgtcgcag
     1081 agcgtgaaat ccaaaaagcg catgaggcta ataaggttcc tttaatttgc ggcggcacgt
     1141 atttttatat ccatgcgctc ttccatggac tcttgccgtc gatggaaatt ccacccagca
     1201 tcacccaaga agtggcggct ttatccgata cggcggcgta tgctaaactc cttcagctcg
     1261 acgccaaggc cgccgagcaa aaccatccac acaacgcggt gcgggtgcgc cgcgcactct
     1321 ctttgtgttt ggcgcatggt ggccccatta gcgcactcgc caaagtaggg ggaatttaca
     1381 acgactacga tatacaactt tttatttttg acgccccgcg cgctcttctt aaagaacgcg
     1441 cgataaccag atctcaaaaa atgctggacg ccggacttat acgcgaaatc gaacagcttt
     1501 ttagcgaaag tgctcgggcg cgtaaagaaa cagcgtggca gaaaattccg gcgctcacgg
     1561 ggattggcat ccgtgaattc tttgaaatgt atgaaaaaac agaaaagaca ccacagcaac
     1621 tttcttcgca agaaatccac accatcaaag agaacatcat acaaaatacg atgcgcttgg
     1681 tgaagcggca acaaacgtgg ttccgcaatg cagcgccgaa acccaaagat acaaaaatag
     1741 ttgacccgtc ttttgaatat gcgcacattg ccacgctcgt gaaggagttc attttaccag
     1801 catcttaaaa tacaattttc aagcgatgta cttatgaata aaaataactt acaagatttc
     1861 attttaaacc aaatccgcaa agagaaaatg gggattacca tttatctctc caatggcgta
     1921 ccgattaaag ggcgtgtggt cagttttgat aatttcacaa ttgtgctcga acacgaccac
     1981 aagcaaacgc tcgtctataa gcatgcggta accaccattt ctcctgaaaa acccatccgc
     2041 ttaaatccca ccgacaccga cgagagcgaa gaaaattaaa atagttgatt tcttgtgatt
     2101 accgattttt ttaggcgact tctacaaatc actgcgcttg ccgcagttgt caccgttgtg
     2161 tatttggatt ttatttttgt ccctaaaggc tataccacat ttttaattgc acccacaaca
     2221 acggggggaa tagacaacca aggtgttttt caatacgctg aagggacgaa ttttgtgccg
     2281 acgctctttg tgccgtatcg tttccaacgc taccaactcg aaacgcaaag caaaatccaa
     2341 gagattgacg tcaaattacc gttgcgttat agcgcgtatt tacgcttgaa cgatcttttt
     2401 tacgtgcagc ttaaaataag aattgaagga tttatttctg agaaaggggc gtatgccgcg
     2461 ctcaaggcgc tgcaatacaa acctaccgac cgcgataaat ttattgagaa gcaacttgcg
     2521 cttgtggttg ctgaatatct actcgacatt aagggcgatg aaaaaaattt agaaaaaaca
     2581 aaaataacgc ttgctgcctt tttaagcccc acaaataaag gagaaattca gtcgcgcctc
     2641 aaccgccaaa tcaacgattc gtggtttact ttgacaagcg tggaactcaa agagatttat
     2701 atgcccgata gcgcgctcta cgccgcgcaa atccgtaatc tagacgaagt ggccacagcg
     2761 gatagagcgg cgctgctttc gcagatttct aaagaagccg agttggcgtt agagcgcaaa
     2821 cgcaatttgg aagatattac taaagctgaa aaaatgggag cactcatcca cgaaaatcca
     2881 tatatactcg actactataa aattgaaaaa attgcaccgc gtgcagggaa tgttatgctt
     2941 gacgtaacct cgaacgcgcg gcatgaaaga ccaactccag cagacttgaa agataaaaga
     3001 gggcgcgatg gcaaaaacga aaacagtgaa ggtggcgaaa tcggtagggc tgagtaaatc
     3061 caccaaagtt tctaaagaat ttgtagagaa cggtaagccg tttgtcatga ttatggcggg
     3121 tggctcgggt acacggcttt ggccgttgag ccggattagc cggcctaaac agttaatcca
     3181 gatcgcgagc tcaaaaacac tcattgaaga agcggtcgat agggcgcgcc tcctcaccac
     3241 gcccgatcgt atttatatcg ggaccaacaa agagctggcg gctcaaatcc aaaaaattgt
     3301 caaactcgat aagaagcaat atctgttaga gcctacacaa agaaataccg ctcccatcat
     3361 tgcgtatttc acctcgttct taaaagcaaa cggtgcagac gatcattcag gggtggtgat
     3421 tttagccgcc gaccaccaca tcgaaccaca ggaggcgtgg gcgaacacgg tgaagctcgc
     3481 gctttcacgc gcgaatgagc ggttatggtg cattggcatt aaacccacac gcgctgaaac
     3541 aggctatggg tatattgaaa ttggtattga agtggctcca cgcatgagta cgatttccag
     3601 ctttcgtgaa aaacccgatt accaaacggc aagccactac caaaccaccg agcggcattt
     3661 ttggaactcg ggaatgtttg ttttttcaat cagccgcatg cgcgaagatt tacgcgtgta
     3721 ccaaagtcaa atgctgaaat tagccgacga atgcgcgcaa agctcagcgg cgttacagaa
     3781 aatttttccc aaaatggaaa atatatccat cgattacgcc atcatggaaa agaccgccga
     3841 gatggcggtg gtggcctgcg acatcggctg gaacgacatc ggttcctgga gcgccctggg
     3901 cgatctggtg gcggccgacg ccagcggcaa ccgggtcgag ggccatgcgc tgctgcagga
     3961 ttccaccaat
//
LOCUS       DAOMPD010000248         3961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9482_length_3961_cov_2.717358, whole genome shotgun sequence.
ACCESSION   DAOMPD010000248 DAOMPD010000000
VERSION     DAOMPD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9482_length_3961_cov_2.717358"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="cas7e"
                     /locus_tag="PLY93_09075"
     CDS             complement(<1..843)
                     /gene="cas7e"
                     /locus_tag="PLY93_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HRP69669.1"
                     /translation="MSKEKIPETTYGSRFLQLHFLTSYPPSNLNRDDLGRPKTALMGG
                     ANRLRISSQSLKRAWRTSDVFSQALAQNRGVRTKNYGKEIFEKLVNDGIKEKDAKTWA
                     KAIAEVFGKLKAEKKDDPLLDLEIEQLAHFSVEEQAAIDTLVKKLIKDKRAPADDELK
                     LLRKENSAADIAMFGRMLASEPAYNFEAAVQVAHAITVHKVAVEDDFFTAVDDLNRGD
                     EDKGAGHMGDIEFGAGLFYLYICINRELLLKNLNGDKTLAENTIAALVEAVATVAPTG
                     KQNSF"
     gene            complement(857..1120)
                     /locus_tag="PLY93_09080"
     CDS             complement(857..1120)
                     /locus_tag="PLY93_09080"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRP69670.1"
                     /translation="MNSLQHETNTLAYEVTNIERLGFWLLIDDKEYFIAFAEYPVFKQ
                     ATVEQILSVERLSPSQFRWPALDADIELEALDNPSQFTLKYTP"
     gene            complement(1101..1316)
                     /locus_tag="PLY93_09085"
     CDS             complement(1101..1316)
                     /locus_tag="PLY93_09085"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRP69671.1"
                     /translation="MPTVYAAGNRRFFFNNREESRRHIHRQTPDGVAKFWLEPIVALA
                     SYYSLKDRELNEIEIIVKEKKDEFIAA"
     gene            complement(1316..1819)
                     /gene="casB"
                     /locus_tag="PLY93_09090"
     CDS             complement(1316..1819)
                     /gene="casB"
                     /locus_tag="PLY93_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HRP69672.1"
                     /translation="MRLFEKDSPSASALLDWWKKLPEHSGDRAALRRAASVVEAVQVP
                     YTHHLISTLKDEQAYGDKIAAICGILSHVRTDNPGQPFARQMASARSGSDQPVISNLR
                     FRRILQYGDITRDILFYQNTVRIVKNLDSRVNIRDLAESLYYWGDFTIKRWAYEYYGT
                     ADNLGDA"
     gene            complement(1816..3405)
                     /gene="casA"
                     /locus_tag="PLY93_09095"
     CDS             complement(1816..3405)
                     /gene="casA"
                     /locus_tag="PLY93_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HRP69673.1"
                     /translation="MNLMRDKWIPVKRRDGTVEDIAPFEVTDKIERNPVVALAAPRPD
                     FNGALIQFLIGLFQTVYAPKNETEWEKRMENPPKPDELQKITEKVAFAFELFGNGPRF
                     MQDMSLDESNRQFGIASLLIDSPGEHTLKLSKDFFIKRHTVARISPRAAAIALFTLQT
                     NSPSGGNGHLVSIRGGGPLTTLVKSEAKNAALWEHIWLNILTKEYFPTKFDDEDWPKI
                     FPWIDNKIGRLWGVGKSATVNLFHPYTVYWACPRRIQLLAETAQDFCDLTNETTDTIV
                     RGYYTKTYGLNYSKGGWIHPLSPYYMNKDMLLPLHPQPGGFLYDNWTSFALDRNSKGK
                     MAQVVSAFYGRKSQEMQSVIHAFGYDMDNMKARCWYEAEIPLFQIAEKDFVAFEEQVA
                     QVLAAAELVKRNLRQAVTLAWFDENQSVKGDFGAIETAFLKNTENNFFDLIQKILKRL
                     QSGRKDFLDLKAAWLKRLNTAALALFDSTTEAGSVEFENIERIVHARKNLIMFNFGAV
                     TKKALGLPVPEKIEKRSKQKA"
     gene            complement(3409..3948)
                     /locus_tag="PLY93_09100"
     CDS             complement(3409..3948)
                     /locus_tag="PLY93_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69674.1"
                     /translation="MPEDAREMIEFVYGDAQDIPAALKDWSDAAEQKENKKESMAVRN
                     MVNFSQGYTATNDADWDDVSAPSRYSEQKSVKIVLARLRDGVCEPWHKAGDSRHSWAA
                     SEVSLPDYIAAAEDHVDEATSTAVACSRETLPSRGKYAIFVVLSKNDNGYFGFAVNSK
                     GQRNKLLYNRKTGLEKESA"
BASE COUNT          908 a         1037 c          907 g         1109 t
ORIGIN      
        1 aaaactattc tgcttacccg tcggggcgac ggtcgcgacg gcctcgacca gtgcggcaat
       61 ggtattttcg gctagcgtct tgtcgccatt cagatttttc agaagcaact cgcggttgat
      121 gcagatatag agatagaaga ggccggcgcc gaattcgatg tcgcccatgt gcccggcgcc
      181 cttatcttcg tcgcctctgt tgagatcatc cacggccgta aaaaagtcgt cttcgacggc
      241 gactttgtgt acggtaatcg catgggccac ctgcacggcg gcctcaaagt tatacgcggg
      301 ttctgaagcc agcatacgac cgaacatcgc tatatcggcg gcggagttct ctttgcgcag
      361 aagtttgagt tcgtcatccg ctggcgcgcg tttatctttg atgagttttt tcacgagagt
      421 atctatggcc gcctgctctt ccacgctaaa atgcgccaac tgctcaatct ccagatcaag
      481 cagcggatca tccttttttt ctgccttcag tttgccgaaa acctccgcaa tggctttggc
      541 ccaagttttt gcgtcttttt ctttaatgcc atcgttcaca agtttctcaa atatttcctt
      601 gccataattc tttgtgcgga cgcctctgtt ttgcgcgagt gcctgcgaga acacatccga
      661 cgtgcgccat gcgcgtttca ggctttgcga agaaatgcgc agcctgttcg ccccgcccat
      721 gagggcggtt ttgggcctgc ccaagtcgtc gcggttgagg ttcgacggcg gatacgaggt
      781 gagaaagtgt agttgaagaa atcggcttcc gtatgttgtt tctggtattt tttcttttga
      841 catggttttt cctttcctat ggggtatatt ttagcgtaaa ctgcgaagga ttatccagtg
      901 cctctaactc aatatccgca tccagtgcag gccagcgaaa ctgcgaaggc gacaaccgct
      961 ccacactaag tatttgctct acggtagctt gcttgaaaac gggatactcc gcaaaagcga
     1021 tgaaatactc cttgtcgtcg atcaacaacc agaaacccaa ccgctcaatg ttggtcactt
     1081 cataggctaa agtgtttgtt tcatgctgca atgaattcat cttttttctc cttgacgatt
     1141 atttcaattt cgttcagttc cctgtctttc aaactataat aagacgccag cgctacaatc
     1201 ggctcaagcc agaatttagc cacgccgtca ggcgtttgtc tatgaatatg ccggcgcgac
     1261 tcttcacgat tattaaagaa gaaacgcctg ttccctgcgg catagaccgt tggcattacg
     1321 catcccccaa attatcggca gtgccgtaat actcatacgc ccagcgcttg atcgtaaaat
     1381 ctccccaata gtacaacgac tcggccaaat cgcggatatt caccctacta tcgaggtttt
     1441 tcacaatgcg cactgtgttt tggtaaaaca atatatcgcg cgtaatatca ccatactgta
     1501 gaatccgacg gaagcgcagg ttgctaatca ctggctgatc cgatcccgat cgggcggagg
     1561 ccatttgtcg ggcgaagggc tgcccgggat tatccgttct tacgtggctc agaatgccgc
     1621 agatagcggc tattttatca ccgtaagcct gctcgtcttt cagtgtgcta attaaatgat
     1681 gcgtataagg tacctgcacc gcctcgacta ccgaggccgc ccgcctgagc gccgcccggt
     1741 cgccgctgtg ttcgggtagc tttttccacc agtcgaggag cgccgatgca gacggagaat
     1801 ctttctcgaa tagtctcatg ctttctgttt gctccgtttt tcgatttttt ccgggactgg
     1861 caaaccgaga gctttctttg taaccgcgcc gaaattgaac ataatcaaat tcttgcgcgc
     1921 atggacgata cgttcgatgt tctcgaattc gacgctaccc gcttcggttg tcgaatcaaa
     1981 taaagccagt gctgcggtat tcaaccgctt cagccacgcg gctttcaaat cgagaaaatc
     2041 tttccgtccg gattgcaagc gcttcagaat tttttgtatc agatcaaaaa aattgttttc
     2101 cgtatttttt aggaatgcgg tttcaatggc cccaaaatca cctttaaccg attgattttc
     2161 atcgaaccac gccagcgtga ccgcctgccg caaattgcgt ttcacgagtt cggcggccgc
     2221 gagaacctgt gcaacctgct cttcaaaagc cacaaaatcc ttttcggcaa tttgaaacaa
     2281 cgggatctcg gcctcatacc agcaacgcgc cttcatgttg tccatgtcat agccaaacgc
     2341 atggattacg ctctgcatct cttgcgattt tcggccataa aaagccgaaa caacttgtgc
     2401 catcttccct ttgctattgc gatcaagcgc aaaagatgtc cagttatcgt acaggaatcc
     2461 tccaggttgg ggatgcaatg gcaaaagcat gtccttgttc atataatatg gactcaaagg
     2521 atgtatccat ccacctttgc tgtaattcag gccataagtt ttcgtatagt agccacgcac
     2581 gatggtatcg gtagtttcgt ttgtcaggtc acaaaagtcc tgtgccgttt ctgccaagag
     2641 ttgaattcgc cttgggcatg cccaatagac ggtgtacggg tgaaagagat tgactgtggc
     2701 tgacttgccc acgccccaaa ggcgtccaat tttattatcg atccaaggaa aaattttcgg
     2761 ccaatcctca tcgtcaaact tcgtaggaaa atattctttg gttaagatat ttaaccaaat
     2821 gtgctcccaa agcgccgcat ttttagcctc gcttttgact aacgttgtta atggcccacc
     2881 accccgaatt gaaacaagat gcccattgcc acctgaaggc gaatttgtct gaagagtaaa
     2941 tagagcaatt gccgccgctc tcggcgaaat tcgagcaaca gtatgtcgct ttatgaagaa
     3001 atcctttgag agtttcagcg tatgttctcc gggagaatcg ataagtaagg aagctatccc
     3061 aaactgacgg ttcgattcat ctagactcat gtcctgcata aacctcggtc cgttgccgaa
     3121 aagttcaaag gcgaacgcca ctttttccgt aattttttgt agctcgtcag gtttgggcgg
     3181 attttccatg cgcttttccc actctgtctc gtttttcggc gcatagaccg tctggaaaag
     3241 accgatgaga aactggataa gcgcgccgtt aaaatcggga cgcggagcgg caagcgcgac
     3301 aaccggatta cgttcgatct tatcggtcac ttcaaacggc gctatatctt cgacagtgcc
     3361 gtcgcgccgt tttacgggaa tccacttgtc gcgcatcaga ttcatcgttt aagcgctttc
     3421 cttctctaaa cccgttttac gattgtaaag tagtttattc cgctgccctt tgctatttac
     3481 tgcaaatcca aagtacccat tgtcattttt tgacaacaca acaaaaatgg cgtactttcc
     3541 tcgggatggc agggtttctc tgctgcacgc gacggcggta gatgttgctt cgtcgacgtg
     3601 gtcttctgcg gcggcgatat agtccggaag actgacttca cttgcagccc aagaatgccg
     3661 gctatcgcct gctttatgcc agggttcgca aacaccatct cgtagcctcg ccagtacgat
     3721 ttttacggat ttctgttcgc tatatctgct gggagcgctg acatcgtccc aatctgcatc
     3781 gttggtcgcc gtatatcctt gagaaaaatt taccatattc cgcacggcca tgctttcttt
     3841 tttattttct ttttgctccg cggcatctga ccaatccttt agcgcagcgg gtatatcttg
     3901 cgcgtcgccg tacacaaact cgatcatttc acgcgcatct tcgggcattt gccagccctt
     3961 g
//
LOCUS       DAOMPD010000249         3953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9504_length_3953_cov_2.307337, whole genome shotgun sequence.
ACCESSION   DAOMPD010000249 DAOMPD010000000
VERSION     DAOMPD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_3953_cov_2.307337"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..876
                     /locus_tag="PLY93_09105"
     CDS             109..876
                     /locus_tag="PLY93_09105"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRP69675.1"
                     /translation="MCEILGMSANVPTDIFFSFTGLIRRGGLTGPHKDGWGIAFYEDK
                     GVRLFHDPEASSQSQIAAFLQTYPIRSETVISHIRKANRGKVALKNTHPFVRELWGRN
                     WVFAHNGQLRGIKKEKLDSFEPVGTTDSEAAFCWLLGKLRRKFKNPPPSHVLIDAVEK
                     LFHELAKRGVANILMSDSDLLFTHCSNKLVYITRRAPFGHAQLLDADVTVDFKKATSE
                     KDIVTILATRPLTGDETWNRYAQGEYRVWRRGECVVN"
     gene            1073..1540
                     /locus_tag="PLY93_09110"
     CDS             1073..1540
                     /locus_tag="PLY93_09110"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRP69676.1"
                     /translation="MIAKITTAGRPGFNSTLITVEADILSGLPNLTIVGLPDHAVRES
                     RERIRSAITNAGFYFPAKQIVINLSPNDTPKEGGLIEAAMATAVLVASGQLPQEAFFE
                     TALLASLSLDGSLKSARALAATAVYTAAHSGIKKIIIPKCALDEIAAPKRGVF"
     gene            2054..>3953
                     /locus_tag="PLY93_09115"
     CDS             2054..>3953
                     /locus_tag="PLY93_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69677.1"
                     /translation="MFRGARLRFTVKIKQKFQGVHLPLWVKGALATTILFIIPFLVLS
                     YRSVHENIRMRKEILYRNFYLKAHAFSFEVRSYLKDKVDLQEENSYLLTRGHFTTGYE
                     LPISEAARLNVLLWLSDKSAGSALIEFFIDPKNNLPRILYVERHKSSRYFIFEANFLA
                     HLLDTSQSLDADNRIFIYNSHEEPFLSNSIENEYRVPTEWMAGIHRLFWQQEINSIQE
                     LKLNEEKFIIARYKIRDLPLVVYLARPYSTAMDEVNETTKNLVLIFLLVAVIVFLMLM
                     YFFRDQVSTLHHLRSFIDGRLSAQKAKRIFLIGDERTEIFADIIAIREKEKRALQERD
                     RADARTKAKGDFLASMSHEIRNPLNAILGIADLLRQKETDAEKRGYLQMIRESGDSLL
                     RIVNDILDISKIENNRLTLEETPFDIRKLIYDLRFFYSAKAETQHNDLIVSVKPGTGP
                     LVLGDPTRLRQILINFISNALKFTQKGRVVLRVRRYLNTESVHFFVHDSGIGISKEGL
                     SRIFAAYEQAEASTTRKYGGTGLGLNIALRLARLMQGSVRCRSRIGKGTSFYCRVHLP
                     QVMAATQAAFVVQDDSADMLARISGMRVLVAEDNEINQMLMVENLKPIVKEVVVVSNG
                     KEILEKVTH"
BASE COUNT         1111 a          864 c          914 g         1064 t
ORIGIN      
        1 ccacctacct taaacacttt ataaaaagtg taacatatgt cttgagggtt tagaccaaat
       61 cggttttgct ttctttacgt cgtatataag cgctatgaca cgccgcatgt gtgcgaaatt
      121 ttggggatga gtgcaaatgt tcccacagac atctttttta gttttacggg acttatccgg
      181 cgcggcgggc taacaggccc gcacaaagat gggtggggaa ttgcttttta cgaagataaa
      241 ggagtgcgcc tttttcacga ccctgaagca agtagccaat cgcaaattgc ggcgttttta
      301 caaacctacc caatccgtag cgaaactgtt ataagccata tacgtaaagc caaccgaggt
      361 aaagtagcgc ttaaaaatac gcaccccttt gtgcgcgagc tctggggacg aaattgggtg
      421 ttcgcacaca atggccaact tagaggaata aaaaaagaaa agctggatag ctttgaacct
      481 gttggtacaa ccgatagcga agcggcattc tgttggcttt taggaaagtt acgccgcaag
      541 tttaaaaatc ctcctccttc gcatgtttta atcgacgcag tggaaaaact ttttcacgaa
      601 ttagccaaac gtggggtagc caatattctt atgagcgact ccgatttgct ttttacccac
      661 tgctcaaata agctcgtcta cattacacgc cgcgccccgt ttggccacgc gcaactttta
      721 gatgctgatg taacggttga ttttaaaaaa gccacgagcg aaaaagatat tgtgacaatt
      781 ttggcgaccc gccccttaac gggagatgaa acttggaatc gctatgcaca aggcgaatac
      841 cgtgtgtggc ggcgcggcga atgcgttgta aactaacagg gagacccgtc gaatttggat
      901 tatgtcacat tagaggataa ctatccctta ttaagtcaca ttaggggata actatcccag
      961 cgagcgattt aagtaaaaaa atcgcgattt tttttgaaaa aactgtactt ttttttccaa
     1021 aaatgagaaa atttacgcgt tttctggtat ttaatatata agagaacaat ttatgatagc
     1081 aaaaataacc accgccggaa gacccgggtt taattcgacg ctcatcactg tagaagctga
     1141 tattttaagc ggacttccta acctcactat tgttggctta cccgaccacg ctgttcggga
     1201 aagccgtgaa cgcatccgct cggcaattac aaacgcagga ttttattttc ccgccaagca
     1261 aatagtaata aacctttcgc ctaacgacac cccaaaggaa ggtgggctca ttgaagcggc
     1321 gatggcaacg gcagttcttg tggcatccgg gcaactgccc caggaggctt tttttgaaac
     1381 agcacttttg gcatctttat cgttagacgg ttcgcttaaa agcgcacgtg ctttggcggc
     1441 aaccgctgtt tacaccgcag cacacagcgg aattaaaaaa ataattatac ctaaatgtgc
     1501 cctcgacgaa attgcagcgc ccaagcgcgg cgtcttttaa actcaaaact tggtcgtaca
     1561 aaatataagc aacgttctgt cgaagttgag cctgtttttg gtaacacctt ttacaataag
     1621 tcccgccgac gcttctggtt acgcggccta cacaaggtgg atattgaagc gggtattgcc
     1681 tttattgcac aaaatattgc taagatacat ttacatttgc taaaaattag gcaagggcct
     1741 cgccatatat taattggtga gtcgacagta agctaaaaag agaaaagctt aagaaaagca
     1801 ccctaagcat aatcgctaaa ttcaacgaaa atagatattc gccgaaatct aaacgcctaa
     1861 ttagacagcc actacatgaa aaaacccctc tctgcgcttg ccctgagtag cccaaagggc
     1921 gtatcgaagg gttagcgggg aggggtggtc agcacagggt gctttttatt gcaaaaaagt
     1981 tgcttcggcg agttggtgcg caggctcctt acttagtgct gtacccgtta tcgattaagc
     2041 aaaagtaagt tatatgtttc gaggcgctcg gctgcgcttt accgttaaaa taaaacaaaa
     2101 atttcagggc gttcacctgc cgctttgggt gaaaggtgcg ctcgcaacga cgatactttt
     2161 tataatccct tttcttgttt taagttatcg ttccgttcat gaaaatatcc gcatgcgaaa
     2221 agaaatactt taccgcaatt tttatttaaa ggcgcatgct ttttcttttg aggtgcgttc
     2281 gtacctcaaa gacaaagttg atttgcaaga ggaaaatagt tatctactga cgcgcgggca
     2341 tttcacgacg ggttatgaat taccaatctc agaagcggcg cggctgaacg ttctgctttg
     2401 gctctccgac aaaagtgcgg ggagtgctct cattgaattt tttatcgacc caaaaaataa
     2461 tttaccgcgt attttatacg ttgagcggca taagagttcg cggtatttta tttttgaagc
     2521 aaatttcttg gcgcatttac tcgacacatc gcaatcgctt gatgccgaca atcgcatttt
     2581 tatctataat tcgcacgaag agccgttcct ttccaacagc atcgagaacg agtatcgtgt
     2641 tcccacagaa tggatggcag ggatacaccg tcttttttgg cagcaagaaa tcaacagtat
     2701 tcaagaactt aaattaaacg aagaaaaatt tattattgca cgatataaaa tccgcgattt
     2761 acctttggtc gtttatttgg cgcgacctta ttccactgca atggacgaag tgaatgaaac
     2821 gacgaaaaac ctcgttctca tttttcttct tgtcgcggtt attgtctttt taatgctcat
     2881 gtattttttt cgcgatcaag tcagcacgct gcatcattta cgctcgttca tcgacggtag
     2941 gctgagcgcc caaaaagcca agcgcatttt tcttatcggc gatgagcgca cggaaatttt
     3001 tgccgacatt atcgctattc gcgaaaagga aaagcgcgct ctccaagaac gcgatagggc
     3061 agacgcgcgc acaaaagcca aaggcgattt tttggcgagc atgagccacg aaatacgtaa
     3121 tcccttgaat gccattttgg ggatcgccga tttactgcgt cagaaagaaa ctgacgccga
     3181 gaaaagaggc tatctgcaaa tgatacgtga atcgggcgat agtctgttgc ggatcgttaa
     3241 cgacattctc gatatctcta aaattgaaaa caaccgcctc actctggaag agacgccatt
     3301 cgatattcgt aagttgattt acgatttacg ttttttttat tcggcaaaag cagaaacgca
     3361 gcataacgac ctaattgtga gcgtgaaacc cgggacaggg ccgctggttt taggcgaccc
     3421 aacgcggttg cgtcaaattc tgattaactt catctcgaac gcgctcaagt tcactcaaaa
     3481 gggacgtgtg gttttgcgtg tgcggcggta cttaaatact gagagcgtgc atttttttgt
     3541 gcacgatagc ggtatcggta tttcaaaaga aggactgtcg cgtatttttg ccgcttacga
     3601 acaggccgaa gcgtcgacga cgcgcaagta tggcggcacg gggttggggc tcaacattgc
     3661 gttacgcttg gcgcgtctga tgcaggggag tgtgcgttgc cgcagccgca ttggtaaagg
     3721 gacttcgttt tactgccgcg ttcatttacc gcaagtgatg gcagccactc aagcagcgtt
     3781 tgttgtacaa gacgattctg ccgacatgct tgcgcgcatt agcggtatgc gggttttggt
     3841 tgccgaagat aatgaaatca atcaaatgct gatggtggaa aacttaaagc cgattgtcaa
     3901 agaagtcgtc gtggtctcaa acggtaaaga gatacttgaa aaagtaaccc atg
//
LOCUS       DAOMPD010000250         3931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9556_length_3931_cov_2.447626, whole genome shotgun sequence.
ACCESSION   DAOMPD010000250 DAOMPD010000000
VERSION     DAOMPD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_3931_cov_2.447626"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLY93_09120"
     CDS             <1..459
                     /locus_tag="PLY93_09120"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRP69678.1"
                     /translation="PHRFLSITKQGVAAIVHTKGNPTAHLILRGSTKGTNYDKESVAL
                     AAKELAQADILPRIIVDCSHGNSNKDFTRQPIVARDVAKQVAEGSETLMGVMLESHIK
                     EGNQKEAPLAKLEYGKSITDACISFETTAPLLDELANAVKKRRTIKKAVA"
     gene            501..1496
                     /locus_tag="PLY93_09125"
     CDS             501..1496
                     /locus_tag="PLY93_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRP69679.1"
                     /translation="MLLSTCSQFAPPTGDVSSQAYTPPHSEIDSLSKRPKIELHGHRG
                     ARGLAPENTWPAFEKAIRLGIDAVELDVQLTRDDAFVIHHDAFTNSKLCQKDNGARIH
                     ARAIQSLTVTELKTLDCGSKKNKLFPEQENIPHTRLITLDEFFIHVRQAESDFPQAKD
                     VRFNIEIKILPNITFTDTYLHNVAKRILQIAAAHSMTKRIMVPSFELRLLPFVKAIDA
                     TVPTAALFISSKRSHRKRTPRDFVERAQKYQADIIAPYYKDITPQLIIYTHRKNLRIF
                     AWTVNEPSDIKKIIAMGVDGIISDYPDRLIRAHKSLHTSIRDSFPDIRKIPTVSP"
     gene            complement(1644..2024)
                     /locus_tag="PLY93_09130"
     CDS             complement(1644..2024)
                     /locus_tag="PLY93_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP69680.1"
                     /translation="MERCRVTISPYESGAYTVVFIGGALNLFTARDVRAQMEPLLKEE
                     RVRVVVDIEGLDTIDSSGIGALVNFILAARKHADARVVFTQPNEIVMRVFEITKLKSF
                     FEIVDDLEAAKVIILNLNSQGIKS"
     gene            complement(2030..2779)
                     /locus_tag="PLY93_09135"
     CDS             complement(2030..2779)
                     /locus_tag="PLY93_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69681.1"
                     /translation="MRGLKASLAVALFSLLPLWSLQKVVLLPIINIENDANFAYLEAS
                     ISGSLRMRLREKLAFDELPEEKWALVAGQHFVLRKDLYTETAAMNLGVFSKQDIVIAG
                     GYKPIIAKGKAQGKSKIHVNVFLLDVKSKKVVINLDFDLPADSELFTAMGQVAEKMEV
                     EASKIILNKEDAARIGGSSTTPFFSDLSVGIGAGGSYYVNGYAKYITPQLPLLNVFLE
                     ARLPRMMASLKGCNQFTTGGGMPVKVFSAYF"
     gene            2902..3663
                     /locus_tag="PLY93_09140"
     CDS             2902..3663
                     /locus_tag="PLY93_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP69682.1"
                     /translation="MRMRILFVIFLLSTASTFLLRAQNPSTPTVQNTTNDTVNPPETS
                     VNTAIAQAGEWHFGSDPTVWVMIFIFNLALFVVLERVYVLRRVRGDAAHLVDYTTEAI
                     AKGEAISTIAENAADARYGLEGRIAAASLKGWDKGEHAMKEYAEATLIGEKRRLEKRL
                     VILSTLGNNTPFIGLLGTVLGIMKAFRDLSTMGDAGPAVVMRGISEALIATAMGLAVA
                     IPVVIAYNALSRMVQDKISQADEIATLLRAIRLNK"
     gene            3666..>3931
                     /locus_tag="PLY93_09145"
     CDS             3666..>3931
                     /locus_tag="PLY93_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP69683.1"
                     /translation="MAFSKNNGGPISDINVTPLVDIMLVLVIILMVTAEFTRYKTIPV
                     HLPKVNAAAVKTEPHRITVTIKKAGDIYVQDKAATDLNDVFEQI"
BASE COUNT         1085 a         1031 c          822 g          993 t
ORIGIN      
        1 ccgcatcgat ttttgtccat caccaaacaa ggagtcgcgg cgatcgtcca cactaaagga
       61 aatcccactg cgcatttaat tttgcgcggc tcaacgaaag ggacaaacta cgataaagaa
      121 agcgtggcct tggcggcgaa agaactcgcc caggcagata ttttgccccg catcatcgtc
      181 gattgcagcc acggcaactc gaacaaagat ttcacccgcc aacccattgt ggctcgcgat
      241 gtcgctaaac aagttgccga agggagcgaa actctgatgg gtgtgatgct cgaaagtcac
      301 ataaaagaag gcaaccaaaa agaagcgccg ctcgccaagc tcgaatacgg taagtcgatc
      361 accgatgcgt gtattagctt tgagacgacc gcgccgcttt tagacgagtt ggcaaatgcc
      421 gtaaagaaac gacgtacaat aaaaaaggcc gtcgcttgac tttcgcatca atagcgcggt
      481 tcctttgcgc cgcgttcttg atgcttctct cgacgtgctc gcagtttgct ccacccacgg
      541 gggatgtttc ttcacaagca tatactccac cacactctga aatcgattct ctttctaaac
      601 ggccgaaaat agaactccac ggccatcggg gtgcgcgcgg cctcgcgcct gaaaatacgt
      661 ggccggcgtt tgagaaagct attcgcctcg gcatcgatgc ggtagaactc gatgtgcagc
      721 tcactcgaga cgacgccttc gttatccacc acgacgcatt cacgaactcc aaactttgcc
      781 aaaaagacaa cggcgcccgg atccacgccc gcgccatcca gagcctcacg gtaaccgaat
      841 taaaaacact cgattgcggc tcgaaaaaaa ataagctatt tccagaacaa gaaaacatcc
      901 ctcacactcg cctcatcact ctcgacgaat tttttatcca tgtacgccaa gcggagagcg
      961 atttccctca agccaaagat gtgcgtttta atatcgaaat taaaattctg ccgaatatta
     1021 ctttcacaga tacatatttg cacaatgttg ccaaacgcat tttacaaatt gctgccgcac
     1081 actccatgac taaacggatt atggtgccgt cgtttgagtt gcgtctactc ccttttgtga
     1141 aagcgatcga cgcgacggta ccgacggcgg ctctctttat ttcgagcaaa cgtagtcacc
     1201 gtaagcgcac accgcgagat tttgtggagc gtgctcaaaa ataccaagcc gatattatcg
     1261 cgccttacta caaagatatc acaccacaac tcattattta cacccaccgt aaaaatttac
     1321 gtatattcgc atggacagtc aacgagccat ccgacatcaa aaaaattatc gccatgggtg
     1381 tcgatggcat tatttcggat tatccggatc gtttgattcg ggcgcacaaa agtctacata
     1441 ccagcatacg tgattctttt cctgacatac gtaaaatacc caccgtatcg ccataaccaa
     1501 actcggcgcc gaagcaatgc acattagtcg cttgttagca ttttaagaga aaaagcgata
     1561 gcttttgcct taccacgaga gagatgttcg accgcaaggc tctgtttctt gaaaccctcc
     1621 tgaattggca tcctgagtgt gtattatgat tttatgccct gcgaatttag gttcaaaatt
     1681 atgaccttcg ccgcttctaa atcatcgaca atctcaaaaa atgattttaa ctttgtgatt
     1741 tcaaaaacac gcatcacgat ttcattcggt tgagtaaaca ccacccgcgc atccgcatgt
     1801 ttccgcgccg ctaaaataaa attgacgagt gcgccaatcc ccgacgagtc aatcgtgtcc
     1861 aagccttcga tatcgacgac gacgcgtaca cgctcttctt tcaaaagcgg ctccatttgt
     1921 gcgcgcacat cacgggcggt aaatagatta agagcgcctc cgataaaaac gacggtgtac
     1981 gcaccgcttt cataggggga aattgttaca cggcaacgct ccattagagt caaaaatatg
     2041 cagagaaaac cttcactggc ataccacccc ctgtggtaaa ttggttgcag ccttttaggg
     2101 acgccatcat acgcggcaaa cgcgcttcta aaaaaacatt caacaacggc aactggggag
     2161 taatatattt tgcataccca ttcacataat aagaaccgcc cgctccgata ccaacgctta
     2221 aatcgctaaa aaatggcgtt gtcgacgatc cgcctatccg tgctgcgtct tccttattga
     2281 ggattatttt actcgcttca acttccatct tttcggcaac ttggcccatc gcagtaaaaa
     2341 gctcgctatc ggcgggcaga tcgaaatcta aatttataac aactttttta gatttcacat
     2401 ctaacaaaaa aacatttaca tggattttac ttttcccttg tgccttacct tttgcgataa
     2461 tgggtttata tcctccggcg ataacaatat cttgttttga gaaaaccccc aaattcatcg
     2521 ccgccgtttc agtgtataaa tcttttcgta aaacaaaatg ttggcctgca acgagcgccc
     2581 atttttcttc gggtaattcg tcaaacgcaa gcttttccct aaggcgcatc ctaagactgc
     2641 cgctaatcga cgcctcaagg tatgcgaagt tcgcgtcgtt ttcgatattg ataattggca
     2701 ataaaacaac tttttgtaat gaccaaagcg gcaatagaga aaataaggct actgctagac
     2761 ttgcttttaa gccgcgcata agccaaaacc tccatagtta tttcaacttg tcaacaacat
     2821 acactttatt tcactcccga gcctgtgcgc caactcgctg aatcgccttt acgccacttt
     2881 tgttttttaa actctgtcat catgcgaatg cgtattcttt ttgttatttt cctactctcg
     2941 acggcatcga cgttcttatt gcgtgctcaa aatcccagta cgcccacagt tcaaaacaca
     3001 accaacgaca cagtcaatcc ccctgaaacc tctgtcaata ctgcgattgc ccaagcgggc
     3061 gagtggcatt ttggttcgga tcccaccgta tgggtgatga tttttatttt taacttagcg
     3121 ttatttgttg tcttggaacg cgtctatgta ctgcgtcgtg tgcgcggcga cgctgcgcat
     3181 cttgtcgact acaccacgga ggcgattgca aaaggagagg cgatatctac aattgctgaa
     3241 aatgccgccg acgcacgcta tggcctcgaa gggcgtatcg ctgctgcttc gcttaaagga
     3301 tgggataaag gcgaacacgc catgaaagaa tacgccgaag caactctcat cggcgaaaag
     3361 cgccgtttag aaaagcgctt ggttattctt tctacattgg ggaacaatac gcctttcatc
     3421 ggtttattgg gcacagtctt gggtattatg aaagcgttcc gtgatttatc cacgatggga
     3481 gatgcaggcc ctgccgttgt gatgcgcggc atctcagaag cgctcattgc caccgccatg
     3541 ggactcgccg tggcgatccc ggtggtgatt gcgtataacg cactttcgcg catggtgcaa
     3601 gataaaattt cacaagccga tgaaatcgca acgctcttac gcgcgatacg cctgaataaa
     3661 taaccatggc attttcaaaa aataatggcg gccccatcag tgacatcaac gtcactccgc
     3721 ttgtcgacat catgttggtg cttgttatta ttctcatggt cacggcggag ttcacacgct
     3781 acaaaacaat tcccgtgcat ttgcccaaag tcaacgccgc tgccgtcaaa acagaaccgc
     3841 atcgcataac agtgactatt aaaaaggccg gagatattta cgtccaagat aaagccgcca
     3901 cagacttaaa cgatgtcttt gaacaaatca a
//
LOCUS       DAOMPD010000251         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9569_length_3925_cov_2.279328, whole genome shotgun sequence.
ACCESSION   DAOMPD010000251 DAOMPD010000000
VERSION     DAOMPD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9569_length_3925_cov_2.279328"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1350
                     /locus_tag="PLY93_09150"
     CDS             115..1350
                     /locus_tag="PLY93_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRP69684.1"
                     /translation="MAYKRLKYSMYRFLITATIFVLLGFVYLGHRLIVFQKPLALRIA
                     AWVFLGSMMANLLWLPFRRWQKQAHIFPTGRWGRFFETNAYNAMGFLSLLLFFTAMSE
                     FVRLAFGFSTTEFMQTLEKKFLVGFNLEQILALVILSSSVVAFLWGRIKVWRGPQYRL
                     VKLVYPLNGKAKRRPHFKKNKTASEKIRIVQVSDLHIGRAIQAVYVQNVVRKINALDA
                     DILCFTGDIGDGKQSELKSAAAPLKNLKAKQGKFLIPGNHEYYWGLHTWLKEFADLQF
                     TTLVNEGKLVTAQGKKIFIAGISDPVAARFGEALPQIPQRKKSHFAVLLTHRPTFVKS
                     ALVYGYDLMLAGHTHGGQYIPWSWVARMVYRFTSGLYRYKNLLVYVSDGTGYWGSPLR
                     LGTRLEITVFDIYINAQEG"
     gene            1353..2189
                     /locus_tag="PLY93_09155"
     CDS             1353..2189
                     /locus_tag="PLY93_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69685.1"
                     /translation="MQDTYKAVTRFADLDTQRHVTSRTYESMALEGRYRILAQAGFPF
                     EKIQSEQIYLRPVSNYAKFHRQQFPAVELNVVTEVNPTTQGTLHWNQKVFEMNGSLVC
                     HLQQETQTIQNGKPLVIPIESQPTEPVLYEKLPPWTGQCERIATYYTIPFAERDFAGK
                     YNLASYWKIFEEGRWGFAEKTGLTYERIVELDTTSFYMGGIASFYTEIAAGRTIEILT
                     WVERIDKIRYWFRQDLVLEGQVLASMRDEQLIVSLSRARPQRATPKFLTYMEKYVEFK
                     PA"
     gene            complement(2411..2764)
                     /locus_tag="PLY93_09160"
     CDS             complement(2411..2764)
                     /locus_tag="PLY93_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRP69686.1"
                     /translation="MRLFYFSTTHSQRGNNPIFFIETLTDGVDLPTLSAVMQKGNFSY
                     NDVDSMHYGFTDKLVWYRYTEATATPLLLMLGNFTLHYVDFYFVKEGKLLSKRSMGQA
                     RPFAGRYRVFIAATR"
     gene            complement(2876..3730)
                     /locus_tag="PLY93_09165"
     CDS             complement(2876..3730)
                     /locus_tag="PLY93_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69687.1"
                     /translation="MHKIIILFISFSLLHAATVITTTDRFTGTITAKTQTSLTMRDED
                     SVTHQIDRSHILKIMDDDGNVVYTAPSLAQDAALPPPLPSAAVAPPPPPQQYDEGSER
                     HDGFFLRMLGGWGSYSFLEKPVYTDGTGRIELSSLIGFFNLQLGVAVVDNFILHGSLS
                     GYSSTDISEGKLDGTTQAFTNKIKANISVIGAGFTYYIMPINLYFGAEVGRATTTITN
                     GSVSFTSEAGVAFNLYIGKEWWVSKNWGLGAALFYHYSTMPDSASGSVIPQITNTVIG
                     IAFSATYN"
BASE COUNT         1046 a          905 c          932 g         1042 t
ORIGIN      
        1 tcttcgcgca aaaaattttg tagttgtttt tctcgttcct cagatgaatt actcattggc
       61 gcgctgtctc tttttttaag aatgaagata gagcttgccc gtcaaccgta aagtatggcg
      121 tacaagcgat tgaaatactc catgtatcgt tttctcatca ccgccaccat tttcgtattg
      181 cttgggtttg tttacctggg gcatcgcctt atcgtctttc aaaaaccgct tgcactgcgt
      241 atcgctgcgt gggtgttttt gggttcgatg atggcaaact tgctgtggct gccttttcgt
      301 cgttggcaaa aacaagcgca tattttccct accggtcgct gggggagatt ttttgaaacc
      361 aatgcgtata acgcgatggg attcctgagt ctacttcttt ttttcacagc gatgagcgaa
      421 tttgttcgtc ttgcttttgg atttagcaca actgagttca tgcagacact tgaaaaaaaa
      481 tttttagtcg gattcaatct cgaacaaatt ttagccctcg tgattttatc gtcttcggtg
      541 gtcgcgttcc tttggggccg catcaaagtg tggcgcgggc cacaataccg tcttgtaaaa
      601 ctcgtgtatc cgttaaacgg taaagccaaa cggcgccccc atttcaaaaa aaataaaacc
      661 gcctctgaga agattcgcat tgtgcaagtg agcgacttac acattggccg tgcaattcaa
      721 gcagtgtacg tgcaaaatgt tgtgcggaaa ataaacgcat tagacgccga catcctttgc
      781 ttcaccggcg acatcggcga tggcaagcaa agcgaactca aaagtgccgc tgcgccgctg
      841 aaaaatctta aagcaaagca aggcaaattt ttaatccccg gcaaccacga atactattgg
      901 ggtttgcaca cttggctcaa agagtttgcg gatctccagt tcacgacgtt agttaatgaa
      961 ggcaaacttg tcacagcgca aggaaagaag atttttattg cggggatttc cgatcctgtt
     1021 gcggcaagat tcggcgaagc gctaccgcaa attcctcaac gcaagaaatc ccactttgct
     1081 gttttattaa cccaccgccc cacgtttgta aaaagcgcgt tagtctacgg ctatgactta
     1141 atgctcgctg gccacacgca cggtgggcaa tacatcccgt ggtcgtgggt cgcgcggatg
     1201 gtgtaccgtt tcacgagcgg gctataccgc tacaaaaatt tgctcgttta tgtcagcgac
     1261 ggcactggtt actggggatc gcccttacgc ttaggcacgc ggcttgaaat taccgttttt
     1321 gatatttaca tcaacgcaca ggaaggataa ctatgcaaga cacctacaag gccgtcacac
     1381 gctttgccga tctcgatacg caacgccacg ttaccagccg cacatacgaa tcgatggcgc
     1441 tcgaagggcg ttaccgtatt cttgcgcaag cgggatttcc ttttgaaaaa atccaaagcg
     1501 aacaaattta tttaaggccg gtgtcgaatt atgcaaaatt ccaccgccaa caattcccgg
     1561 ctgtggagtt aaacgtcgtc accgaggtaa atcccactac gcaaggcacg ctccactgga
     1621 atcaaaaagt ttttgaaatg aacggctctc ttgtgtgtca tttgcagcaa gaaacccaaa
     1681 cgatacaaaa tggcaaaccg cttgtcatcc ctattgaaag ccaaccgaca gagccggtgc
     1741 tttacgaaaa attaccgcca tggacgggac agtgcgaacg tatcgcaacc tactacacca
     1801 taccttttgc cgagcgtgat tttgcgggga aatataatct cgcctcgtat tggaaaattt
     1861 ttgaggaagg ccggtggggt tttgccgaga aaaccggcct cacatacgag cgcatcgtcg
     1921 agctcgacac cacttctttt tacatggggg gtatcgccag tttttatacc gaaatcgcgg
     1981 ctgggcgcac gattgaaatt ttgacgtggg tggagcgtat cgacaagatt cgctattggt
     2041 tccgccaaga tttggtgttg gaagggcaag tgctcgcgtc gatgcgcgac gaacagctta
     2101 tcgtgtcgct ctctcgcgcg cgcccccaac gggcaacgcc gaagtttttg acgtacatgg
     2161 aaaagtacgt ggaatttaaa cctgcttaaa ttcctgaatc aaactttccc aaggtgtacc
     2221 cttgagctgt tctttcagtg ctgcaaagtg gcggctttgg acacgagtaa ggctcttttg
     2281 ttttcgtgtt caatcgcacc cgttaagccc atacctactt ctttggtacc agcaacaagg
     2341 caagtgctcg aagggtttgc ccttgaacca tcgctaaatc atcggcgttg atgagctcga
     2401 tggggcggtg tcaacgggta gcggcgatga aaactcgata acgccctgcg aaggggcgcg
     2461 cctgtcccat cgagcgtttt gaaagcagtt taccttcttt tacaaagtag aaatcgacat
     2521 aatgcagcgt gaaattaccg agcatgagaa gcaaaggtgt tgccgttgcc tcagtgtaac
     2581 gataccaaac cagcttgtct gtgaagccat agtgcatcga atccacgtcg ttgtaagaaa
     2641 aatttccctt ttgcatcact gcagaaagag tgggtaaatc gactccatcg gtaagagtct
     2701 ctataaaaaa gatggggttg ttcccccttt gactatgtgt ggttgaaaag taaaataggc
     2761 gcaatattgc ggatggccgt tgatggtgga gctacaacgc gggatgctgt cttgttatag
     2821 caagcagcaa aacagattaa cgtaagaaaa aaaatcttac gtccacgcac gcgctttaat
     2881 tatacgtcgc gctgaaggcg atgccgatga cggtgttggt aatttgaggt ataaccgaac
     2941 cagaggcaga atcgggcatt gtgctataat gataaaataa tgccgctcca aggccccagt
     3001 tctttgaaac ccaccattct ttacctatgt agaggttaaa cgcaacacct gcttctgaag
     3061 taaatgaaac agaaccgttt gtaatggtag ttgttgcacg acctacctct gcgccaaaat
     3121 ataaatttat tggcataata taataggtaa aacccgcacc gatgacacta atgtttgcct
     3181 taattttatt tgtaaaagct tgtgtcgttc catctaattt gccttcagaa atatccgttg
     3241 aagaatatcc actgagcgaa ccgtgaagaa taaaattatc aaccacggca acaccaagct
     3301 gtaaattaaa aaatcctatg agtgacgaca gctctatacg ccccgtgcca tcggtataaa
     3361 caggtttctc taaaaacgaa taagaccccc aaccacccaa catgcgcaga aaaaagccgt
     3421 cgtgtctttc agacccttcg tcgtattgtt gcggtggtgg cggtggtgcc actgcggctg
     3481 aagggagtgg gggtggaagt gctgcgtctt gagcgagcga tggcgcggtg tagacgacgt
     3541 tgccgtcgtc atccataatt tttaaaatgt ggctgcgatc gatttggtgc gtaacgctgt
     3601 cttcatctct catggttaga ctcgtttgtg tcttcgctgt aatcgtgcct gtaaaacgat
     3661 ccgtcgttgt gataacggtt gctgcgtgga gaagagagaa gctaataaag aggataatga
     3721 ttttgtgcat aagctccatt ttattgcgga tattcaaggt gtcacgcaaa aatgaggact
     3781 gacgagatag ggcagaaatt gatggtttat acgtacagaa gagagttctc cctctaattt
     3841 ttccgatttt gactactcca atactcggga tacatagtta cctcttaatg cgacataatc
     3901 tgaaaaatca atatagttac cccct
//
LOCUS       DAOMPD010000252         3919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9581_length_3919_cov_2.804089, whole genome shotgun sequence.
ACCESSION   DAOMPD010000252 DAOMPD010000000
VERSION     DAOMPD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9581_length_3919_cov_2.804089"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLY93_09170"
     CDS             <1..869
                     /locus_tag="PLY93_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP69688.1"
                     /translation="FVSIPAPYGKLSMQVAGLDEGDAILFIHGWPDSHDTWSHQMEYF
                     SKNYRVGALDLPGVGGSDAPTERVGYHIDNILPLIQKAIETLGGKKTHLVAHDWGAII
                     SWVFASRPEYAKYIASYTAVSGPHPGLARDYLFSRLKSFDPQKIADALRQMAKSWYIY
                     LFQLPFVADYVLGNFGRAVWPRALMQGGVPEKDPMLNASNEEIARIAVNPLNLYRELI
                     QGKWLEVPTRIDTKVQVIIPKEDLAITPEIYSNVLEVAPHAVMHYLDANHWVQRSHPS
                     LVNQYLEMFIRQ"
     gene            898..1266
                     /locus_tag="PLY93_09175"
     CDS             898..1266
                     /locus_tag="PLY93_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69689.1"
                     /translation="MQTQTLHNTKEWDVLFTASNVWRVGVYRPRFRSRSEISELEEHT
                     CPEAFLLIAGSVTLFYRKDDGSLAEKKLIPMELTIVTEPHAGFSPNLDGVAFVIENAV
                     FTTTYTDVHTSQVTRQVSVK"
     gene            complement(1271..2122)
                     /gene="purU"
                     /locus_tag="PLY93_09180"
     CDS             complement(1271..2122)
                     /gene="purU"
                     /locus_tag="PLY93_09180"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRP69690.1"
                     /translation="MQLQSLILLVHSPDAPGIVARISNLIFETQGNITQLDQHTTTSE
                     EGQFFMRVRFDLPRENFTSFEEKWQALSRAINAKWQLFDIEKPQQMAILVSKADHCLY
                     DLLYRYASRELIVDIPFIISNHRDLEAVAEKYNIPFVHIPIAKETKKEAERELLKQVH
                     EKSDFMVLARYMQILSPEFIETYGKAIINIHHSFLPSFKGAYPYERAYERGVKLIGAT
                     AHFVTHELDEGPIIEQMVMRVSHRDSADDMRRMGKNIEKLALASAVRCYVERRIIRYE
                     NRTVVFT"
     gene            complement(2130..3185)
                     /locus_tag="PLY93_09185"
     CDS             complement(2130..3185)
                     /locus_tag="PLY93_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69691.1"
                     /translation="MAYAFERFVFYQTTVAERERVAALQILYPQLTRDRVLFIDSDLE
                     LETLEPNDFLVCANDSLHYAYPRAFAMGYVGLLTFADFIKTPAHFIDLQASTIFGEVV
                     LAFLSEPLKKQAVRLFSLFGFNPIAVDSLPILLKELKHRASLVVFDQDMPTQPKQRGA
                     ENRTKIFSLLRAERKQRRQLSVIIIKDFDQGSLFSDMTTQAKDVSNAMLSPQEFLEFI
                     RNYLCDFHTAHATWKLKTGTNTGLAKANYTGRPTAHLGLNNIKEAFEIFQDRSYQEFI
                     LLREQLLAETLDLSVKMRATDWLFDKQLAQAADSAQKNLLIRAQLPRALEKSSLQIQV
                     NEGKPSEEPSTGDVMFN"
     gene            3343..3414
                     /locus_tag="PLY93_09190"
     tRNA            3343..3414
                     /locus_tag="PLY93_09190"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3375..3377,aa:Cys,seq:gca)
     gene            3479..3562
                     /locus_tag="PLY93_09195"
     tRNA            3479..3562
                     /locus_tag="PLY93_09195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3513..3515,aa:Leu,seq:taa)
     gene            3536..>3919
                     /locus_tag="PLY93_09200"
     CDS             3536..>3919
                     /locus_tag="PLY93_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRP69692.1"
                     /translation="MPVQLRPRVHRLIDAHCHLDMVLERGLGVETITATLEQNGVDAL
                     VEIGADKNAMLWARTLAHTATRFDVYYTIGHHPGEAATEDFEFGLRFIEENKDDARFV
                     AVGEIGLDYHYYSDQAEIQKKVFIEF"
BASE COUNT         1031 a          924 c          971 g          993 t
ORIGIN      
        1 aatttgtctc tatccccgca ccgtatggca aactttcgat gcaagtcgcc ggtctcgatg
       61 agggcgacgc catcctcttt atccacggtt ggccggattc gcacgatacg tggtcgcacc
      121 aaatggaata tttctcaaaa aattaccgtg tcggcgctct cgatcttccc ggcgtcggtg
      181 gttcggatgc acccacagag cgcgtcggct accacatcga taacatattg ccgctcatcc
      241 aaaaggctat cgaaacattg ggtggaaaaa aaacacactt ggtcgcgcac gattggggcg
      301 ccatcatcag ttgggttttt gcatcacgcc ccgaatacgc caaatacatt gcgtcgtaca
      361 ccgcagtgag tggccctcac cccggtcttg cgcgggatta ccttttttca cgccttaaat
      421 cgttcgatcc acaaaaaatc gcggacgcat tgcggcagat ggcaaaatcg tggtatatct
      481 atttattcca attgccgttt gtggcggact atgtgctcgg caattttgga cgcgccgtgt
      541 ggccacgcgc gctcatgcag ggcggcgtac ctgaaaaaga ccccatgctg aacgcgagta
      601 acgaagaaat tgcgcgcatc gccgtcaatc cactcaattt gtaccgcgaa cttatccaag
      661 gcaaatggct cgaagtgccc acgcgcatcg acaccaaagt gcaggtaatc atacccaaag
      721 aagatttggc aatcactccc gaaatttatt cgaatgtgct cgaggttgct ccacacgcgg
      781 tgatgcacta tttggatgca aaccactggg tgcagcgatc gcacccgtcg ctggtgaatc
      841 agtacttaga aatgtttatc cgtcagtaaa aaactgacat aacacacaaa gcggtgcatg
      901 cagacacaaa cgctccacaa cacaaaagag tgggatgtac tcttcaccgc atcgaatgta
      961 tggcgggtgg gtgtttaccg cccgcgtttc cgctcacgca gtgaaatttc cgaactcgaa
     1021 gaacacacct gccccgaggc gtttttgtta atcgcgggaa gcgtaactct cttttatcgc
     1081 aaagacgatg gctcgcttgc tgaaaaaaaa ttaataccga tggagctaac aatcgtcacc
     1141 gaaccgcacg cgggattttc cccgaacttg gatggtgtcg catttgttat cgagaacgcc
     1201 gttttcacca ccacctatac cgacgtgcat acttctcaag tgacgcggca agtgtcggtg
     1261 aaatgataat ttacgtaaac acgacggtgc ggttttcgta gcggataatc cgccgttcca
     1321 cgtaacaacg cactgccgag gcgagtgcga gtttttcaat gtttttaccc atgcgccgca
     1381 tatcgtcggc gctgtcgcgg tgtgaaacac gcatcaccat ttgttcgatg atagggcctt
     1441 cgtcgagttc atgcgtgaca aaatgcgccg ttgcaccgat aagtttaacc ccacgctcat
     1501 acgcgcgttc gtaagggtat gcccctttga acgacggcaa aaaactgtgg tggatattga
     1561 tgatggcttt accgtatgtc tcgataaact ctggagataa gatttgcata tagcgtgcca
     1621 acaccataaa gtcggatttc tcgtgaactt gctttaagag ttctcgctct gcttcttttt
     1681 ttgtttcttt tgcgatggga atatgtacaa aaggaatatt atatttttcc gccacagctt
     1741 ctaagtcgcg gtggttggag ataataaatg gaatgtcaac aatgagttcg cgcgacgcat
     1801 agcggtagag taaatcataa agacaatggt cggctttaga aacgagaatt gccatttgtt
     1861 gtggtttttc tatgtcgaaa agttgccact tagcgttgat tgcgcgcgac aacgcttgcc
     1921 atttttcctc aaaagaagta aaattttcac gcggtaaatc aaaccgaacg cgcataaaga
     1981 actgcccttc ttcagatgtc gttgtgtgct ggtcgagctg ggtgatattc ccctgtgttt
     2041 caaaaattaa attagaaata cgcgccacaa tgcccggcgc gtcgggtgaa tgaacaagaa
     2101 ggatgagaga ctgcaattgc atgaactcac taattaaaca tgacatcgcc cgtcgacggt
     2161 tcttcagagg gcttgccttc gttgacttga atttgcaaag acgatttttc caaagcgcgt
     2221 ggtagttgtg cgcggatcaa taaatttttt tgcgcggagt cggcggcttg cgcaagttgt
     2281 ttatcaaaaa gccaatcggt tgcgcgcatt ttcacggata aatccaacgt ttcggcgagt
     2341 aactgttcgc gcaagagtat aaattcttgg taggagcgat cttgaaaaat ttcgaacgct
     2401 tcttttatat tgttgaggcc caagtgtgct gtcggcctgc cggtgtaatt tgcttttgca
     2461 agacctgtgt tggttcctgt ttttaatttc cacgtcgcgt gcgcggtgtg gaaatcgcaa
     2521 aggtagttgc ggatgaattc taaaaactct tgcggcgaga gcatcgcgtt tgagacatct
     2581 ttagcttgcg tggtcatgtc gctaaaaaga ctgccttggt cgaagtcttt aataataatc
     2641 accgaaagtt gccgccgctg ttttctttca gcgcgtagga gtgaaaaaat ttttgtacgg
     2701 ttctctgcgc cgcgctgttt aggttgcgtc ggcatgtctt ggtcgaagac aaccagtgac
     2761 gcacggtgct ttaactcttt aagtaaaatg ggtaacgaat ctacagcgat gggattaaag
     2821 ccgaatagag aaaataatcg caccgcttgt ttcttgagcg gctcgctcaa aaaggccaac
     2881 accacttcgc cgaaaatggt cgatgcttgc aagtcgataa aatgcgcagg ggttttaata
     2941 aaatcggcaa aagtcaaaag acccacataa cccatcgcga acgcgcgggg ataagcgtag
     3001 tggagcgaat cgtttgcaca aacaagaaaa tcgtttggct caagtgtttc gagctctaaa
     3061 tcactgtcga taaacaggac acgatcgcgc gtcagttgtg ggtagagaat ttgcaacgcc
     3121 gccacacgct cgcgctcggc gacggtcgtt tggtaaaaaa caaatcgctc aaacgcgtac
     3181 gccatagatg tgacataatt cagaactgtt tttcaccgtg tcaagccgac atagagttaa
     3241 gaccgaaact cgcacaggat atatctatgg gttagacagg tgagaatttc ataatgattg
     3301 acgccgtatc ttttattgat tcttacaaca cagtaaaaaa atggtgtcgt agccaagtgg
     3361 taaggcagag ctctgcaaaa gctctatgcg ccggttcaaa tccggccgac acctcaggga
     3421 agatttctta tcgatttgcc cacgaagatc ggcaagttga gtaagaataa ccaaaagagc
     3481 ccgtgtgatg gaattggtag acatacagga cttaaaatcc tggggcagca atgccgtgcc
     3541 ggttcaactc cggccacggg tacatcggtt aatcgacgcg cactgccatt tggatatggt
     3601 gctggagcgt ggattaggag ttgaaactat tacagcgacg ctcgaacaga acggcgtcga
     3661 cgctctggtt gaaattggtg cggataaaaa cgcgatgctg tgggcgcgca cgctcgcaca
     3721 taccgcaacc cggtttgacg tgtactacac cattggtcac cacccggggg aagccgccac
     3781 cgaagatttt gaatttggtc ttcgcttcat cgaagagaac aaagacgacg cccgttttgt
     3841 ggctgtgggg gaaatcggcc tcgactacca ttattattcc gaccaagcgg aaatccaaaa
     3901 aaaagtattt attgaattt
//
LOCUS       DAOMPD010000253         3893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9657_length_3893_cov_2.610735, whole genome shotgun sequence.
ACCESSION   DAOMPD010000253 DAOMPD010000000
VERSION     DAOMPD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_3893_cov_2.610735"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLY93_09205"
     CDS             complement(<1..211)
                     /locus_tag="PLY93_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP69693.1"
                     /translation="MSVRENLSLGLRLRKTPKAEITARTQEAAKVLGLEPYMDRKPRE
                     LSGGQRQRVALGRAIVRRPQVFLFDE"
     gene            complement(108..470)
                     /locus_tag="PLY93_09210"
     CDS             complement(108..470)
                     /locus_tag="PLY93_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP69694.1"
                     /translation="MASVNFEGVHKIYPNGYVGVEDFNLTIGDGEFMIFVGPSGCGKS
                     TTMRMVAGLEDISRGTITIGDKVVNTVPPRDRDIAMVFQDYAHERARKFELRAAASKN
                     AQGRNYGAHPRGGQSAGP"
     gene            complement(476..>913)
                     /locus_tag="PLY93_09215"
     CDS             complement(476..>913)
                     /locus_tag="PLY93_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69695.1"
                     /translation="DYDSGTNARQFIKGSRVNFNADYYVRLGHNGGSDYGNLYKHNNA
                     NYWDTGTDLWTTCTGCERYVGWDTTQQITEVKIPFAFIGNPSSLRIIAFVTNSTNDYV
                     YSLLSGQAGLIGEDNTPDTSTQKYFKLDRASILKPNDASLLAN"
     assembly_gap    914..923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<924..2888)
                     /locus_tag="PLY93_09220"
     CDS             complement(<924..2888)
                     /locus_tag="PLY93_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP69696.1"
                     /translation="MKRTSLNIFTLLTVIALAACGGSKGGEASSTGGGGGGAPSDTTP
                     PTVGTAITFSGISDTDITVSWGAASDNVTSQANLQYRLVKATSSAAIDNVAEVDAITG
                     VNLIADYTSALLTKSVTGLTASTTYHFAVVVKDTAGNKAIYAPQSQATAAAGTTASPT
                     FNPTPGAFNVDQNLVMASATSGATICYTDNGVTPACDGPKTGCTTGTKYTSAVVVSTT
                     QTYKALACKAGNTDSSETSGTFTVDKTAPTIASTTPADAATSIAENSTIAVTFSEAMD
                     VSTIKSVSGTTTCDINSQSIQVSLDNFATCIGMTSATPSTSDNTTFTMTPAAALQYNT
                     TYMVRVKTLVKDAVGNALTSEYAHTTGFKTGLGPALTINNVSPGTGTYSTTQSVSVTS
                     VEGGAVICYTTDGNTPATSAPGTCSTGSQINEGASIPISDTATLKILLTKVGHSDSAV
                     SSYTYTFPPKVTSTSPADGTPGFDPTVGKIDINFNRTMSRASASFNTTTGACTGNIQV
                     SADDFTTCIGITIPAGDSATLSATAVALPLPSVTTYKVKIENTLKSTAGDNVQPAQMA
                     IGVTTRYYRIITIDGADTDWSADELVMANGGAGLSWAVTWDSANFYLRLKDKDFKSDL
                     SARLDIYFDTTPGFELDINAATDAKGTEYAF"
     gene            3095..>3893
                     /locus_tag="PLY93_09225"
     CDS             3095..>3893
                     /locus_tag="PLY93_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69697.1"
                     /translation="MLDIVLLTALIIAVLTFFLCLNQITKRIKKPAEASLPHENTSLF
                     TAYLLRITISALLLFLLFAFLSHYYFDQLHDFFTLASMALISGCFVVFLIFTLLSEKL
                     QRYIDARIDLLRKETMSRLLRHFLYVGALTGIATVLLSAGYTFLVAQQPQMVIDFFLL
                     PIYASALFLFWLRFRVALVARASDYAFELIKRQEAALPLAGEHNPLWKLRLDFAAINR
                     FLFYHLEFFILCVVALLVAGQTEKFIAFTNALSGFMALSVFSIGICAA"
BASE COUNT          865 a          962 c          948 g         1108 t
ORIGIN      
        1 gctcgtcgaa taaaaacacc tgcggacggc gcacaatcgc acgccccagc gccacgcgct
       61 gccgttgccc gcccgaaagc tcgcgcggtt ttctatccat atacggttca aggcccagca
      121 ctttggccgc ctcttgggtg cgcgccgtaa tttctgcctt gggcgttttt cgaagccgca
      181 gccctaagct caaattttcg cgcacgctca tgtgcgtagt cttgaaagac catcgcaatg
      241 tcacggtcgc gtggcggcac ggtgttgacc accttgtcgc cgatggtgat agtgcctcgg
      301 cttatatctt caagccccgc taccatgcgc atcgtggtcg acttgccgca acccgagggg
      361 ccgacaaaaa tcataaactc gccgtcgccg atggtaagat tgaaatcctc taccccaaca
      421 tagccgttgg ggtagatttt atgcacgccc tcgaagttaa ccgatgccac gcggactaat
      481 ttgccaagag cgaagcgtcg ttgggtttca agatcgatgc gcggtcgagt ttgaaatatt
      541 tttgcgtcga cgtgtcgggg gtattgtctt caccgataag ccccgcttgt cctgaaagaa
      601 gagagtacac gtaatcatta gtgctattcg ttacgaaagc gataatccgc aacgacgaag
      661 gattgccaat aaaagcgaac ggaattttaa cctcagttat ttgttgtgta gtatcccaac
      721 ccacataccg ctcacaacct gtgcaagttg tccacaagtc agtccctgta tcccagtagt
      781 tggcgttatt atgtttgtaa agatttccat aatcgctacc gccgttatgc ccaagccgta
      841 cataataatc cgcgttaaaa tttacccgcg agcccttaat aaactgccga gcattggtgc
      901 ctgaatcgta atcnnnnnnn nnnaaaagca tactcggtgc cctttgcatc ggttgctgca
      961 ttgatgtcca gttcaaaccc tggggtagtg tcgaaataaa tgtctaaccg tgcggatagg
     1021 tcactcttaa aatctttgtc ttttaatctt aaataaaaat tcgcgctatc ccacgtaacc
     1081 gcccagctta gccccgcacc gccgttggcc ataaccagtt cgtccgcaga ccaatcggtg
     1141 tcggcaccgt cgatggtgat aatgcggtag tagcgtgtag taacccctat ggccatttga
     1201 gcaggttgaa cgttgtcgcc tgctgtggac tttagggtat tttcgatttt caccttatac
     1261 gtggttacgc tcggcaacgg cagtgcgaca gcggttgcac tgagtgttgc actgtcgccc
     1321 gccggtatag ttatgcctat gcacgttgtg aaatcatccg ccgaaacttg gatgttgccc
     1381 gtgcaagcac cggtggtggt gttgaaactg gcggacgcgc ggctcatggt tctgttgaag
     1441 ttgatgtcaa ttttacccac tgtggggtcg aaaccaggcg tgccatcagc agggctggtg
     1501 cttgtgactt taggcggaaa cgtgtacgtg tacgaactca ctgcagaatc ggaatgcccc
     1561 actttcgtga gtaaaatttt gagcgttgcg gtgtcgctaa tgggtatact tgcgccttcg
     1621 ttaatttggc ttcctgtact gcaagtgccg ggggcagagg ttgcgggtgt attgccatcg
     1681 gtggtgtagc aaatcaccgc accgccttcg acggaggtta cgctcaccga ctgcgttgtg
     1741 ctataggtac cagttccagg actaacattg ttaattgtga gtgcaggccc taaaccagtc
     1801 ttaaatcccg ttgtgtgcgc gtactcgcta gtaagcgcat tccccaccgc gtctttaacg
     1861 agagttttga cgcgtaccat ataggtcgta ttgtactgga gagctgccgc aggcgtcatg
     1921 gtaaatgttg tattgtcgct ggttgaggga gtagcagatg tcatgccgat gcacgttgca
     1981 aaattgtcta acgacacttg gatcgattgg ctgttgatat cacacgttgt cgtgccgctc
     2041 actgatttaa ttgtcgatac atccatcgcc tcagaaaaag taacggcgat ggttgaattt
     2101 tcagcaatac tcgtcgctgc atcggcgggt gtcgtcgatg cgattgtggg agcggtttta
     2161 tcgacggtaa aagtgccgct ggtttcagaa gagtcggtgt tacccgcttt gcacgccaaa
     2221 gccttgtacg tttgtgtggt ggagaccacc actgcgctgg tgtatttggt tccggtggta
     2281 catcctgttt tggggccgtc gcatgcaggc gttacgccgt tatcggtata gcagatcgtt
     2341 gcgcccgatg ttgccgatgc catcaccaaa ttttggtcta cgttaaatgc ccccggagtt
     2401 ggattaaacg tgggtgaggc agtggtgccc gctgcagcgg ttgcttgact ttgtggagcg
     2461 taaatcgctt tgttgcctgc ggtatcttta acaaccacgg caaaatgata cgttgtcgag
     2521 gcggtaagtc ctgtcacgct ttttgttaat aaagcggatg tatagtctgc gataagattt
     2581 acccctgtga tggcatcgac ttctgcaaca ttatcaatcg ctgcgctcga tgtagccttc
     2641 accaagcggt actgtaaatt ggcttggctg gttacattgt cgctcgccgc cccccatgaa
     2701 acggtaatgt cggtgtctga aatgccgcta aacgtgatag ctgttccgac ggtgggtggc
     2761 gttgtatcgc tgggagcacc tccgccgccg ccaccggtgc tacttgcttc gccgcctttg
     2821 ctgccgccgc acgcggctaa ggcgatgacg gttaataaag taaagatatt aaggcttgta
     2881 cgtttcataa atttctccaa cttgagaaca aacgtggcaa gccgcttaca gacagtcaag
     2941 cgagtaatga attgaatcat cgatgaggat gcgcaatttt tggcactgcc aaagttaaat
     3001 ttatttgaaa tagaatgtat atctatctca aataaggtgc gagtaaattc gtataacact
     3061 ttaccgccct gaaataagtg tgtgcaaggc ttcaatgctc gacatcgttt tactgactgc
     3121 acttattatc gctgttctta ccttctttct ctgcctcaat caaattacaa agcgtataaa
     3181 aaaacctgct gaagcatcgc tgcctcacga aaacacttcc ctgtttacgg cgtatctcct
     3241 ccgaatcaca ataagcgcgc ttcttctatt cttacttttc gcttttttga gccactacta
     3301 ctttgatcaa ttacatgatt tttttacact cgcctctatg gcgcttatta gcggttgctt
     3361 tgtggtgttt cttattttca ccttactctc agaaaagttg caaaggtata tcgatgcgcg
     3421 tatagaccta ctacgcaaag aaacgatgag tcgcctattg cgtcattttt tatacgtggg
     3481 agctctcact ggcatcgcca cagttcttct ctctgcaggc tacacttttt tggtcgcaca
     3541 acaaccacaa atggtgatcg atttcttttt actccctatc tacgctagcg cactttttct
     3601 tttctggcta cgctttcgag tagcgctcgt tgcacgcgcc agtgattacg cctttgaact
     3661 cattaaacga caggaagcag cattacccct cgctggagaa cacaaccctt tgtggaagtt
     3721 acgccttgat tttgcagcaa ttaatcgatt ccttttttat cacttagaat tttttatttt
     3781 gtgtgtcgtc gcgcttcttg tcgccgggca aactgaaaaa ttcattgcct ttacaaacgc
     3841 gctttctggt tttatggctc tttctgtttt ttctattggt atttgcgccg cga
//
LOCUS       DAOMPD010000254         3852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9795_length_3852_cov_2.219910, whole genome shotgun sequence.
ACCESSION   DAOMPD010000254 DAOMPD010000000
VERSION     DAOMPD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_3852_cov_2.219910"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLY93_09230"
     CDS             complement(<1..378)
                     /locus_tag="PLY93_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004466560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRP69698.1"
                     /translation="MQIVDRDGTPEEAKKLGLLDGEFDMIVERLGRSPSFTELAMFSG
                     MWSEHCSYKNSILILKTLYSESERMLAKPGEENAGALKINDKQAVVFKVESHNHPSAV
                     EPYQGAATGVGGIMRDIFTMGARP"
     gene            611..>3852
                     /locus_tag="PLY93_09235"
     CDS             611..>3852
                     /locus_tag="PLY93_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69699.1"
                     /translation="MKWSTGAKIVTVAIITLLFITCAPRTKQTQFSKNKMAEAGQNYT
                     PDQLRAALGAANYDALITGIGSDNLNLLSYGIGVSNMTALMNAVTGPGKLVALMSDGP
                     HSKKLTAIEVLDFLNKLDDACQLSSLMPNGNDTIQKMAQMVNGVTVAGMEGIKNIVHG
                     VQIQSGDKFGAPYSTNAMARLGMLVALLNEGASPSIMAVLINNVAAPGGAFSDSGTAK
                     LIRMVNETQDLWSLYEIIDKTSSIANITDVMLGLNGNIYCSKPEYTSKSTCTSNGGTW
                     TNTNCTTGTPSSPHTTQAACIGAGGTWTSDGIENMTQVLNQLDAVCTDLAYTNAKDCR
                     NNGKAWHTIASKVPVIVNNISVVPNMYTIVNNLTNDGKPFNPMLGPPDGVDIMVATLN
                     NVYNAGMGDTGTYAQYGLNGVKRLAYMVNQLDQTPVFSNDSNFDDTTGGFNCATGNFN
                     NYLNWAFTDNGPAAAPWNNKSWRSTNAKAGTGTCSLVSNDATADIYNATQSAELLVNL
                     TTTGNLTFAINTFGLGSFDIVRVFIDDSKVGEFTKSGSDTFTTQSYAIATGIKRIRFD
                     IQRIPGSAGKVYIDTVTLPGDKGAPRSAAEKTAIMMNNLYITGAVTNVADLLNNTTAT
                     STPAGCWNSSPISCVPTNDNGLDALIYAANRSEYPGGAAGAGVKGYVTISMASPAKVT
                     WFGHSLANGTAIKLSSSGTLPAPFATNMVYYVTNAAANNFELALTPSAASINTSTAGS
                     GIHMAISQPRLAVTVNEISNLNEMYMVLNNLTTFNSYQQLVALMDYVEIPKNVPNLIN
                     GLGAGGGAKTASILSGLTAAGTSNMLRTLAEPTHDGANISDVADLINGATTTSHVAVA
                     LSKLTLSGNIYMGKYTTVTANAATDRIAWNGHNYREQSPVDFDTTGTLPAPLKPGKKY
                     YMRNVGANDFQVSLTPGGAPIDITTNGAGTHTAYSKGELFYDGPVLGASGGKKFAQFF
                     SSVNAKATAMSQNCAGTPGDFNSNFCIKFHFVRIVNDLAGSSEGPVTIARIVGNLRPD
                     AGVGGGTGITRMTEALYDMKTMAAPVYNNALSNATADQFGRMTTLMTDMGV"
BASE COUNT         1019 a         1014 c          876 g          943 t
ORIGIN      
        1 gggccgtgcg cccatcgtaa aaatatcgcg catgataccg cccaccccgg ttgcggcgcc
       61 ttggtacggt tcgaccgccg acggatggtt gtgcgattct actttgaaaa ctaccgcttg
      121 cttatcgtta atcttgagtg cacccgcgtt ttcttcgcca ggcttggcga gcatgcgctc
      181 cgactcggaa tagagagttt taagaatgag aatagagttt ttatacgaac aatgctccga
      241 ccacatcccc gagaacatcg ccagctcagt aaaagacgga ctgcgcccga ggcgctcgac
      301 aatcatgtcg aattcgccgt ctaaaagacc aagcttcttg gcttcctcag gggtcccatc
      361 acggtctacg atttgcatac gtcgaagcat accttttaga taggtggaca aggaatagta
      421 gagagcctgt gagcctgtgc gagttccagt ctgggcactc agaaacatgg gttctgggtt
      481 aagaaaaaca aaagtttcac aacaactccc ttatgcgtcg cactaaacac ttgtaaacct
      541 ttttggcctc aatttgtact ttgtgaacat atattcatga taggttagcg atacttataa
      601 cgagggatat atgaaatggt caactggggc taaaattgta actgtagcta ttataacgtt
      661 actttttatt acttgcgcac cacgcaccaa acaaacgcag ttttctaaaa ataaaatggc
      721 cgaggcaggc caaaactaca cgcccgatca gttgcgcgct gcattgggcg cggccaatta
      781 cgatgcactc attaccggca tcggttccga caatctcaac ttattgtctt atggtattgg
      841 tgtctctaac atgactgctc tcatgaatgc agtcacaggc ccgggaaaac tcgttgcact
      901 catgtccgat ggccctcact ctaaaaaact gactgcgatt gaagtcttgg attttttgaa
      961 taaactcgac gatgcctgcc agctctcgag tttaatgccc aacggcaacg acaccattca
     1021 aaaaatggcg caaatggtga atggtgtcac cgtcgccggc atggagggca ttaaaaacat
     1081 cgtccacggt gttcaaattc aatcgggtga taaatttggt gctccctatt ccacgaacgc
     1141 catggcgcgg ttgggaatgt tggtcgctct gcttaacgag ggtgcatctc ccagtatcat
     1201 ggcagtcctc atcaataatg ttgccgctcc cggtggcgct ttttccgatt ccggcaccgc
     1261 gaaactgatt cgtatggtca acgaaacaca ggatttgtgg agtctctatg aaattattga
     1321 taaaacgtcc agtatcgcca atatcaccga cgtgatgctc ggcttaaacg gcaatatata
     1381 ttgctcaaaa cccgagtaca catcaaaatc aacttgtaca tcaaacggcg gtacatggac
     1441 gaacaccaac tgtactactg gcacgcccag ttcgcctcat acaacccaag cagcctgtat
     1501 tggtgccggt ggtacgtgga catcagacgg tatcgaaaac atgacacagg tactaaacca
     1561 gctcgacgcc gtctgtaccg atttagcgta caccaacgcc aaagattgcc gcaacaatgg
     1621 caaagcttgg cacacgattg cttctaaagt tccagtgatt gtgaacaaca tctcagtggt
     1681 gcccaacatg tacaccatcg tgaataatct caccaacgat ggtaaacctt tcaacccgat
     1741 gctcggcccc cccgacggtg tcgatattat ggtggcaacg ctcaacaatg tgtataacgc
     1801 tggtatgggc gacaccggca cgtacgcgca gtatggacta aacggcgtca aacgcttggc
     1861 gtacatggtg aatcagctcg atcaaacacc ggtttttagc aacgacagta acttcgacga
     1921 taccaccggc ggttttaatt gtgcaacagg gaatttcaat aactacctca actgggcgtt
     1981 taccgacaac ggtcctgccg cagctccatg gaacaataaa tcgtggcgtt cgacaaatgc
     2041 caaagctgga accggtactt gttctttagt cagtaacgat gcaactgccg acatttataa
     2101 cgccactcaa tcggcagaac ttcttgttaa tcttacaacc acaggtaacc tcacatttgc
     2161 aatcaataca tttggtctcg gctcattcga tattgtccgt gtttttatcg acgattcaaa
     2221 agtcggtgag tttacgaaat cgggtagcga tactttcacg actcaatctt atgccatcgc
     2281 aacgggcatt aagcgtattc gttttgacat acaacgaatt cccggcagcg ctggaaaagt
     2341 ctatatcgac acggttactc tccctggaga caaaggcgct cctcggtcgg cagccgagaa
     2401 aaccgctatc atgatgaaca acctttatat caccggtgcg gtaaccaacg tggctgactt
     2461 gctcaacaac accacggcaa cctcaactcc tgccggttgc tggaacagct cccccattag
     2521 ctgtgtgccc accaacgaca acggtttaga cgcgcttatt tacgccgcca accgctctga
     2581 ataccctggt ggcgccgctg gagcaggcgt caaaggctat gtaacaatct cgatggcaag
     2641 ccctgcaaaa gtaacttggt ttggccacag ccttgccaac ggcactgcaa tcaagctttc
     2701 atccagcggt actctgcctg cccctttcgc cacaaacatg gtctattatg tcacgaatgc
     2761 agcggcgaac aattttgaat tagcattaac cccctctgcc gcttcgatca acacctcgac
     2821 agccggtagt ggtatacaca tggcgatttc acaaccccgt cttgcggtga ctgtgaatga
     2881 aatctctaac ctcaatgaaa tgtacatggt cttaaataat ctaaccacat tcaattcgta
     2941 ccaacagctc gttgcactca tggactatgt agagataccc aaaaatgtcc ccaaccttat
     3001 caatggtttg ggtgcaggcg gcggcgcaaa aacggcaagt atcttgagtg gacttactgc
     3061 agctggtacc tcaaacatgt tgcgcaccct cgctgagccg acgcacgacg gcgccaacat
     3121 ctctgacgtg gccgacctca tcaacggcgc caccaccacc agtcatgttg cggtggcgct
     3181 ctcgaagctg acgctgtcgg gtaatattta catgggaaag tatacgacgg taactgccaa
     3241 tgccgcaacc gatcgtatcg catggaatgg ccataattat cgggaacagt ctcctgttga
     3301 ttttgataca accggcactc ttcccgcacc attgaaaccc ggcaagaaat actatatgcg
     3361 caatgtgggt gcgaacgact ttcaagtcag tttgaccccc ggtggtgcac ccattgatat
     3421 aactactaat ggcgccggta cacacactgc ttattcaaaa ggcgaacttt tttatgatgg
     3481 acctgtgctg ggagcttcgg gcggtaaaaa atttgcacag ttttttagct cagtcaatgc
     3541 caaagctact gctatgtcac aaaattgtgc tggaactccg ggagatttta actctaattt
     3601 ctgtatcaag tttcacttcg tgcgcattgt gaatgactta gcaggctctt cggaagggcc
     3661 agtaacgatt gcccgcattg tgggtaactt acgccccgat gcaggtgttg gtggcggtac
     3721 aggtattact cgtatgaccg aggctctcta tgacatgaaa acaatggccg cccctgttta
     3781 taataacgca ctttccaacg ccaccgccga ccaatttggt cgcatgacga cgctcatgac
     3841 cgacatgggg gt
//
LOCUS       DAOMPD010000255         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_9912_length_3818_cov_3.069625, whole genome shotgun sequence.
ACCESSION   DAOMPD010000255 DAOMPD010000000
VERSION     DAOMPD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_3818_cov_3.069625"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2795
                     /locus_tag="PLY93_09240"
     CDS             <1..2795
                     /locus_tag="PLY93_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP69700.1"
                     /translation="YTFRIPSIAAAARIFVNAHREIENWVVSEDLRQVVPYLRTQYLQ
                     LPLAGPVELVVQVSNAHFKKGGIWQPISLAHSQAMDGFRLSGVLADVFVIVALLTFAL
                     YYIFLSLLRNPEKSALYYGIFCLVVAIESLFSGERLFSVLFPSIPWDIAYRIELFCNF
                     TDALFFYFFLRYFFPEIKQSSVLKGMAGVAILFSLATLTLPVSLLRFLKEPFQIFIAT
                     AILYVFFLLIKQAIRKIYGARIFLLGLVVVAIGFSNDVLKNTEHITTPYLFSYSILIF
                     TMLQAVLISGKSMRLFHENAELSKKLIEADKFKDEFFAQTSHELRTPVQAIVQTVENI
                     RRGNSGSIAEKAQETLKVVEETGRRLIYLIDDISDFVELKHSEVRLRVEAIALKKIAE
                     PIFRLALGLAENRPISIFDEIPAELKDVRVDVTRFQQILLNLISTAIRYTHSSTIHVK
                     SEIAENGFNLKIIYNGVQPSEAYIDDEETNRDELGALVVRRLAHLMYGRYYYQKIAES
                     QHALCLFLPYTDINKLKKILSAPPLKKEYKTDDGKKLDATSISPHNTEFKETSVLLVS
                     DNAGQIRLLQEQLASIGIYSQSAKNGAEAMGLLQNEHNIALIMSDILLVDVSGIDLTR
                     NIRMLYDIGILPIILIADKNQAGIAASAFTVGANDIIRKPFEKVEVLARVKNMLLQRE
                     ASLARENYRSLARELEIARNIQESILPIPQPKNNLYKIEAVCLPARSIGGDLYDFIED
                     ETSLGVLLADVAGHGIPAALYAAMLKISFHNLRDQVRHPEKLLKSINEVMLESKERTF
                     ISCTYTYLDFKNKRLLHANAGHLPLFVQDPNQTAVKKIQPPGSVLGVRSAATLTVEMI
                     HLKPGTRLTLFTDGVIELTNRKGEFFEEEGLIQVLQEMRNAPLSALKDELLERMREFA
                     SMENFLDDVTFLVIDV"
     gene            2829..3806
                     /locus_tag="PLY93_09245"
     CDS             2829..3806
                     /locus_tag="PLY93_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69701.1"
                     /translation="MSAKIVVFEPDGIYRTIIENILLKNDYKPYFVDSGAECKVVVDE
                     VRPEIIILDIKAAKSHDFRILSDLKILTKAPIVLTTEENVDDYMTTLANKGVTIVLPK
                     PIKSSELSRVLANLLTVDVRVWFGLHNYLPDLRKLRKICLKRSTQTRSCIKTITNEMH
                     AWGFNFTQETEMDLVWQEILINAIYHAHGYTAEKKERVPIELPDPYQVEVVYGANENA
                     FGIAVRDFMGTLTPEIVIHALKQTIEQQRLIERSVKTGEDISEQILDRGRGIDLIRQL
                     SGEYYFIMDPGNSTEVIIIYDRYYEKDDSVGSLKIFDLSYHLGATPFSR"
BASE COUNT         1103 a          821 c          810 g         1084 t
ORIGIN      
        1 tgtatacttt tcgtattcca tcgatagcag cggctgcgcg tatttttgtc aatgcgcaca
       61 gagaaattga aaactgggtg gtctctgaag atttaaggca agtcgttccc tatttacgca
      121 cacaatattt acaactgccg ttagcaggcc ctgtggagtt ggtcgtacaa gtcagtaacg
      181 cgcattttaa aaaaggcggt atttggcagc cgatttcatt ggcgcactcg caagcaatgg
      241 atggttttcg tctcagcggt gtgctcgccg atgtttttgt tatcgttgct cttttaacat
      301 ttgcgcttta ctatattttt ctttcgttgc tgaggaatcc tgagaaatcc gcattgtact
      361 atggaatttt ttgcctcgtt gtggccatcg aaagtctttt ttcgggagag cggctcttta
      421 gcgttctttt cccttcgata ccgtgggata ttgcgtaccg aatagaatta ttctgtaact
      481 ttaccgacgc actctttttc tatttttttc tacgatattt ttttccagaa ataaagcaaa
      541 gcagtgtgtt aaaaggaatg gctggtgtag caatactttt tagtctcgca acgctcacct
      601 tacctgtttc gttgctacgt tttttaaaag agccgtttca aatctttatt gctacggcaa
      661 ttttatacgt ttttttccta cttataaaac aggcaatccg taagatctac ggtgcacgaa
      721 tttttttatt ggggttggtg gttgttgcta taggttttag caacgatgtt ttgaaaaaca
      781 cagaacatat tacaaccccg tatttatttt cctactcaat actcattttc accatgttgc
      841 aagcagtatt gatttcaggt aaatcgatgc ggctttttca cgaaaacgca gaactttcta
      901 aaaagcttat agaggccgat aagtttaaag atgaattttt tgcgcaaaca tcgcatgaat
      961 tacgcacgcc cgttcaagca atcgtgcaaa cggttgagaa tatacggcga gggaattccg
     1021 gttctatcgc tgaaaaagcg caagagactc tcaaagtcgt cgaagaaacc ggtagacggc
     1081 tgatttatct catcgacgac atcagtgatt ttgtcgaact aaaacatagc gaagttcgtt
     1141 tgcgcgttga agcgattgcg ctaaaaaaaa ttgccgagcc tattttccgt ctcgcactgg
     1201 ggttggccga aaacagacca attagcatat ttgatgaaat acccgccgaa ttaaaagatg
     1261 ttcgcgtcga cgtaactcgt tttcaacaaa tactcctaaa cctgataagc acggcgattc
     1321 gttacactca ttcgtcgaca attcatgtaa aaagtgagat cgctgaaaat gggtttaact
     1381 taaaaattat atataatggc gttcaaccca gcgaagcgta tatcgacgac gaagaaacga
     1441 atcgcgacga actgggcgcg ttggttgtgc gtcgattggc gcatctcatg tacggccgct
     1501 attattatca aaaaattgcc gaatcacagc acgcgctatg cttgttccta ccttacactg
     1561 atataaataa acttaaaaaa attttgagcg ctccgcccct gaaaaaagaa tataaaaccg
     1621 acgacggtaa aaagttagat gcaacctcaa taagccctca caataccgaa ttcaaagaaa
     1681 catccgtcct tttggtgagc gacaacgcag gacaaattcg ccttctacaa gagcaactgg
     1741 cgtcgatagg tatttattcg caatcggcaa aaaatggcgc cgaagcgatg ggattgcttc
     1801 aaaacgagca caacatcgcg cttattatga gcgacatttt gctcgttgat gtttcaggca
     1861 ttgatttaac gcgtaacatt cgaatgcttt acgatatcgg tatactacca attattctta
     1921 tcgccgataa aaatcaggcg ggtatcgccg ccagcgcatt taccgtcggt gcaaacgaca
     1981 ttatccgcaa accttttgaa aaagtagagg tgttggcgcg cgtgaaaaat atgcttttgc
     2041 aacgcgaagc ctcgctagcc cgcgaaaact accgctcgct tgcccgcgag ctcgaaattg
     2101 cgcgtaacat tcaagaatcg attttaccga taccgcagcc taaaaataat ctctacaaaa
     2161 tcgaggcggt gtgtttaccc gcgcggagta tcggcggcga tctttacgat tttatcgaag
     2221 atgaaacatc gttgggagtt cttttggcgg atgtcgccgg ccacggtata cccgctgcac
     2281 tttatgcggc gatgcttaaa atctcgtttc ataatttacg cgatcaagta cgccatccgg
     2341 aaaaattact gaaaagtatc aacgaagtga tgctcgaaag taaagaaaga acatttatta
     2401 gctgcaccta cacctacctg gatttcaaga ataagcgcct cttgcacgca aacgccggcc
     2461 acttacccct tttcgttcaa gaccccaatc aaacggcagt caagaaaatc caacccccag
     2521 gtagtgtgtt gggtgttcgc agtgcggcaa cactcacggt tgaaatgata cacttaaagc
     2581 cgggcacccg cctcactctc ttcacggatg gtgtgattga gcttacaaac cgcaagggcg
     2641 aattttttga ggaagaaggg ttaatccaag tcttgcaaga gatgcgtaat gcaccgttga
     2701 gcgctctcaa agatgagcta ctcgaacgga tgcgggagtt tgcgtccatg gaaaattttc
     2761 tcgatgacgt tacctttctt gtaatcgacg tatagatttt ttttatttca accgatactt
     2821 taaaaggcat gtcagcaaaa attgtagttt ttgagcccga tggtatttat cgcacaatca
     2881 tcgaaaatat actattaaaa aacgactata aaccctattt tgtcgatagc ggcgctgaat
     2941 gcaaagttgt cgtcgatgaa gttcgtcctg aaattattat tttagatatt aaagcggcta
     3001 aatcgcacga ttttagaatt ttatctgatt taaaaatatt gaccaaagcg ccgattgtac
     3061 tcaccaccga agaaaatgtc gacgattata tgacgacgtt agcaaataag ggcgttacaa
     3121 ttgtactccc caaacccatc aaatcgtcgg agctctcgcg tgtattagca aatttgctga
     3181 cggttgacgt gcgagtgtgg tttgggctcc acaattattt accagactta cgtaaattac
     3241 gaaaaatttg tcttaagcgt tcaacgcaaa cacgttcatg catcaaaacg attactaatg
     3301 aaatgcatgc ttggggtttt aattttactc aagaaacaga aatggactta gtgtggcaag
     3361 aaatccttat caacgctatt taccatgcac acggctatac tgccgaaaaa aaagaacgcg
     3421 taccgattga attacccgac ccttaccaag ttgaagtagt gtacggtgca aatgaaaatg
     3481 cattcggtat tgctgtgcgt gatttcatgg gaacactcac tcctgaaatt gtgattcacg
     3541 cgttgaagca aacgattgaa caacaacgtc tcatcgagcg ttccgtaaaa acgggagaag
     3601 atataagtga acagattctc gaccgtggac gagggatcga tctcatccgt caattatcgg
     3661 gtgagtatta ttttatcatg gatccaggaa attccaccga agtaattatc atttatgacc
     3721 gatactatga aaaggatgac tcggtgggtt cgcttaaaat ttttgacctt tcttatcatt
     3781 tgggcgccac ccctttttcc cgataaagcg ggcgcacc
//
LOCUS       DAOMPD010000256         3777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10034_length_3777_cov_3.015046, whole genome shotgun sequence.
ACCESSION   DAOMPD010000256 DAOMPD010000000
VERSION     DAOMPD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_3777_cov_3.015046"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLY93_09250"
     CDS             <1..671
                     /locus_tag="PLY93_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69702.1"
                     /translation="YYTFMRRHDAQTTQKENADACSHCGTPFALEGELTKCKSCGAVE
                     GSGTFDWVLAEITQEIEYNGLTSRKDIAEPWSADRIEDRASYLFWRNILAQCKGDAQV
                     LQRDATDEYREKGIQKTVYYQAAVGAADLTSLKERDGVLYASVRIKWSAAKNPKAEPT
                     HAESILTLAAKREAAEATTFADPGCASCGAPLPESDSATCAYCHAPVTKKNSDWLLVD
                     VQNG"
     gene            782..3286
                     /gene="pheT"
                     /locus_tag="PLY93_09255"
     CDS             782..3286
                     /gene="pheT"
                     /locus_tag="PLY93_09255"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRP69703.1"
                     /translation="MKLSLNWLNEFVDLADVHPNELVDLLTMKTCEVEGFTNFMQHLA
                     EIPVAHIDALEKHPDADKLKICKVNTGKEVIQIVTGASNVEQGKKFPVAQVGVTLPDG
                     PHKDGIHAGAGHKASMPNGRTMAKAKLRGVESFGMLCSGSELGITQLNYAPAKIDGLM
                     PLPDDWEVGTPLSHYLGGHDIIFDIDNKSITHRPDLWSHFGFAREIAALLDKPLKKNP
                     EKTTAQSIGIVPKKIPLPKIQIQGDAAITYNAAEISQFKVAPSPLLRQMRLLSIGQKP
                     INNIVDISNYVLFEMGQPNHAFDRAKIQGPVQVSLARANEKIDLLDGTTHTLPIRLIV
                     IRDHDKPVALAGVMGGAGTETSNNTTSIFLESATFHRSHIRMAVSKTGIRSDSSQRFE
                     KAQDPSKALPAIYRFAEILNEEQPGIELSEVASVSAIEFDTPVTMKVSNAFINQKLGK
                     IQSRSLSNAEVLTRLGFIIEEQNEVLTVTVPSYRKWFDITRPEDLVEEIGRFIGYKEI
                     TIEAPLVACETPRTQNFSRALEHRLRALAAERFHLNEVMSYAFHSEKDLNADESFTTA
                     AQAMKMKNAINDELNFMRISPLPGLLRAVQTNYREEENVRLFEIEKIFLPSSDERLAR
                     NETYFFAAALTSTKSAADALTDLSAMAGEILTHAGLLRHEQVFSNTPSAIFHPGRSGT
                     AGAHDKKIIRFGQVHPRLLAEYGIKEDMYYLDTKLDDLLSLTKNISHYRAPFTHPGVE
                     FDLTLVIPRHGTFAELKKAAGFVDPLVRRDLDKTVMVCFDYVGTFEGGNLADGEKAVS
                     VRVEWRNPTRTLAGDEIKKLQDGLIHKMQKVGFKLR"
     gene            complement(3383..>3777)
                     /locus_tag="PLY93_09260"
     CDS             complement(3383..>3777)
                     /locus_tag="PLY93_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRP69704.1"
                     /translation="LVVATACFQAVNVIGYPEARIILSQAATYLATSPKSNAAYRAIN
                     NAQNLVRETGNLPIPLALRNAPTALMKDAGYGKGYEYSHDQPRNFSQQEYLPDAISGR
                     TLYEPGENTREKEIRATLKKLWGEKYGY"
BASE COUNT          998 a          969 c          930 g          880 t
ORIGIN      
        1 agtactacac gtttatgcgc cgccacgacg cgcaaacgac gcagaaagaa aacgccgatg
       61 catgcagcca ttgcggtacg ccgtttgcac tcgaaggcga gctcacgaaa tgcaagagtt
      121 gcggcgccgt cgaaggctcg ggcacattcg attgggtctt ggcagaaatc acgcaagaga
      181 ttgagtacaa tggcctgaca agtcgaaaag acatcgccga gccatggagc gccgaccgca
      241 tcgaagaccg tgcgtcttac cttttttggc gcaatatact cgcacaatgc aaaggagacg
      301 ctcaagtttt gcagcgtgac gcaacagatg aatatcgtga aaaaggtatt cagaagacag
      361 tgtactatca agctgctgtg ggtgctgcgg acttaacgag cctcaaagaa agagacggcg
      421 tgctttacgc cagtgtacgg ataaaatggt ctgcggcgaa aaatcccaaa gcagaaccaa
      481 cgcacgccga atcgattttg acgctcgccg caaaacgcga agctgccgag gcgacgacgt
      541 ttgccgaccc gggctgcgct tcgtgcggcg cgccgcttcc cgaaagcgat agcgcaacat
      601 gcgcgtattg ccacgcgccg gtgacgaaga agaactcgga ttggctactg gtagatgtgc
      661 agaatgggta atgaagagtc tcatgcgcta actcgcgtag cgagttttcg catgcgacta
      721 catacccacc gtaaagagct tgtccgcctg tgttttcatt cgagttaatc gaaatataat
      781 catgaaactt tctttgaact ggctcaatga attcgtcgac ttggcggatg tacatcctaa
      841 cgaactcgta gacttactga ctatgaaaac ctgcgaagtt gagggtttta ccaactttat
      901 gcaacacttg gctgaaattc ctgtagcgca tatcgacgcg ctcgaaaaac atcccgatgc
      961 ggataaactt aaaatttgca aagtcaacac cggtaaagag gtgattcaaa tcgtcaccgg
     1021 cgcatcgaac gtcgaacaag gtaaaaaatt tccggtggct caagtgggcg tcactttacc
     1081 cgacggcccg cacaaagacg gcatccatgc cggtgcaggc cacaaggcgt ccatgcccaa
     1141 tggccgcact atggccaaag caaagctgcg cggcgtagaa agtttcggca tgttgtgttc
     1201 gggtagcgag ttggggataa cgcaactgaa ttacgcgcca gcgaaaatcg atggcctcat
     1261 gccgttgccc gatgattggg aagtcggcac accgctttca cactacttag gtgggcacga
     1321 tattattttt gacatcgata ataagtcgat tacgcaccgc cccgacctgt ggagccactt
     1381 tggttttgcg cgcgaaatcg ccgcactcct cgacaagcct ttaaaaaaga atcctgaaaa
     1441 gaccaccgca cagtcgattg gtatcgtacc gaagaaaatc cctctcccta aaattcaaat
     1501 ccaaggcgat gcggcgatta cctataacgc ggcagaaatt tcccagttca aagttgcccc
     1561 ttcaccgctt ttacgccaaa tgcggcttct gtcgattggg caaaagccca tcaacaatat
     1621 tgtagatatc tccaactacg ttctttttga aatggggcaa cccaaccacg cgttcgaccg
     1681 agctaagatt caaggtccgg tgcaagtgag ccttgcgcgc gcgaatgaaa aaatcgacct
     1741 cctcgacggc accacgcaca cgctgccgat acgccttatt gtcatccgtg accacgacaa
     1801 gcccgtcgct ctcgcggggg tgatgggcgg tgcaggcacc gagaccagca acaatacgac
     1861 gtcaattttt ttagagtcgg ccacattcca ccgctcgcat atccgcatgg cggtatcgaa
     1921 aacaggcatc cgcagcgact catcgcagcg ctttgaaaaa gcgcaagacc cctcaaaagc
     1981 actgccggcc atttatcgtt ttgctgaaat actcaacgaa gaacagcccg gcatcgagct
     2041 atccgaagtg gcgagtgttt cggcgattga gtttgacaca ccggtcacga tgaaagtctc
     2101 aaatgctttt ataaatcaga aactcggtaa aattcaaagc cgctcactct caaacgccga
     2161 ggtgctcact cgcttgggct tcatcatcga agagcaaaac gaagtgctga ctgtcactgt
     2221 gccgagttac cgcaagtggt tcgacatcac ccgtcccgaa gatttagtcg aagagattgg
     2281 tcgtttcatt ggttataaag aaattacgat tgaagcgccg ctcgtcgcgt gcgaaactcc
     2341 gcgtacccaa aatttttccc gcgcgttgga acaccgcttg cgcgccctcg ctgcggagcg
     2401 ttttcatttg aacgaagtga tgagctatgc gttccactca gaaaaagatt tgaatgccga
     2461 tgaatctttc accacagcag cacaagcaat gaaaatgaaa aacgccatca atgacgaatt
     2521 aaacttcatg cggatttccc ccctccctgg tttattgcgc gcggtgcaaa ccaattatcg
     2581 tgaagaagaa aacgtgcgcc tttttgaaat tgaaaaaata tttctaccgt caagcgacga
     2641 aaggctcgca cgcaacgaaa cgtatttttt tgcagccgca ctcacttcga ccaaaagtgc
     2701 tgctgatgca ctcaccgatc tttcggcgat ggcaggagaa attttaaccc acgctggtct
     2761 tttacgtcac gagcaagttt tttcaaacac gccctcggca atttttcacc cagggcgtag
     2821 cggtactgca ggcgctcacg acaaaaaaat tatccgcttt ggtcaagtcc atccccgcct
     2881 tttagcggag tatggaatta aagaagatat gtactatttg gatacaaagc tggacgatct
     2941 tttatcgctc acaaaaaata tatcgcacta tcgcgccccg tttacgcacc caggagtgga
     3001 gtttgatttg acgttggtaa tcccccgaca cggtacattt gccgagctga aaaaagcggc
     3061 gggatttgtc gatcctctcg tgcgccgcga cctcgacaaa accgtaatgg tgtgcttcga
     3121 ttatgtaggc accttcgagg gcggtaattt agccgacggc gaaaaggcgg tttcggtacg
     3181 tgtcgaatgg cgcaacccca cacgcacgct tgcgggcgac gaaattaaaa agctgcaaga
     3241 cggcttaatt cataaaatgc agaaagtggg gtttaagttg agatgataac ttatcaccgg
     3301 ggggatgtct aaaaagggcg aatttaacgc ttatttttaa ttttttcttt gttttaattt
     3361 catacctttt tggttttctt tttcagtagc cgtacttctc accccagagc ttttttagcg
     3421 tagcacggat ttctttttcg cgggtgttct ctcccggttc ataaagtgtg cggccagaaa
     3481 tggcatcggg taaatactct tgctgtgaaa aattcctcgg ttggtcgtgc gagtactcgt
     3541 accctttgcc gtacccagcg tctttcatta gcgcagtggg tgcgttccgt aaagcaagcg
     3601 ggatggggag attgccggtt tcacgcacaa gattttgagc gttgttaata gctcgatacg
     3661 ccgcattgct tttcggtgaa gtcgctaaat acgtggcggc ttgcgaaaga atgatccgcg
     3721 cttcgggata gccaatcaca ttgaccgctt ggaaacacgc cgtcgccacc accaacg
//
LOCUS       DAOMPD010000257         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10079_length_3762_cov_2.793364, whole genome shotgun sequence.
ACCESSION   DAOMPD010000257 DAOMPD010000000
VERSION     DAOMPD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10079_length_3762_cov_2.793364"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..2113
                     /locus_tag="PLY93_09265"
     CDS             101..2113
                     /locus_tag="PLY93_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRP69705.1"
                     /translation="MNLLFKESSPYLKQHVNNPVHWQPWGEAAFEEAKRRDVPVFLSI
                     GYSTCHWCHVMAHESFEDNEVASILNEHFVSVKVDREERPDIDQVYMHICYAMTGQGG
                     WPLTFVLFPDKRPFFAGTYLTKTSMYGRLGLIELLKNVTAVWRNQRSALEAEASEALG
                     EIRGKKLEVAGKAELSHIKNAVAFFEKRFDAKHGGFSKKTKFPSFHNLIFLLRYSHFY
                     NHEAALNMVEKTIYEISAGGIYDQIGFGIHRYSTEPTWHLPHFEKMLYDQALYIFTLA
                     ELYQKTKREFYRTQLLRTVQFVETTLACPERGYYTALDADTEHEEGKYYIFTYHELRE
                     ILTDEELHFIVRHLTIQERGNFHDESTGEEVNANIFHWRENPTGGVSLNWDALPLFEQ
                     FEPIRQKILSARKKRTLPHLDNKILTDTNALYLAALAKAAFVLNDVALKKTAQSLAHF
                     LLARIENGTLWHVKNNHGKIDGTLDDYAYTAWGLWEYYSLSRESASLLAATSLTDTLL
                     GNFAADGGFYFTDNKKETEFVIRTREFFDGALPTGNAVALDNLWRLFQTTNENKYRTA
                     YDKLLALFGGFTAEIPTACAHALGVFMKTLVSKELVFTQESAALDAALASQYSPSLAL
                     VKATPELAAQFPYLNAYQSDSEKYWLCRNFACEAPVASVDKIFPQL"
     gene            2172..3290
                     /locus_tag="PLY93_09270"
     CDS             2172..3290
                     /locus_tag="PLY93_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRP69706.1"
                     /translation="MALDDHAGLEDIEVLKSSICELNQKELRFRGYSINDLAEKATFE
                     EVVYLLWNDALPSRQELSDFTNALRAEYNLPREIIEHINHVPREAHPMAVLRTLVSSL
                     GLYDEDAANRSVPAQQKKAIKLIAKIPLIVAAIARHRNAKANVEANPELGIAANFLYS
                     LTSAIPDTKTARLIETTLILYAEHELNASTFAARVTAATEADTYSCIVSALSTLQGGL
                     HGGANQRSIEMLLTIANPESVDEYIAPRLTKKEIIMGFGHRVYKNGDPRVPILRTLCE
                     SLAKSQNEKKLFQTAKQLEEEMLKRKSLHANVDFYSGLAFYLLGLSADIFTCVFAMAR
                     SAGYLAHIAEQWQRNKLIRPRAVYEGKKNLAFVPIDKR"
     gene            complement(3294..3632)
                     /locus_tag="PLY93_09275"
     CDS             complement(3294..3632)
                     /locus_tag="PLY93_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRP69707.1"
                     /translation="MQQVFTNEEENIAAQFLREKGFIVLNRNNRSYGAEIDLLCTRKE
                     DEAYYIFEVKKVARQRGLAYPRISQKQLSRLRKAAQRMQEKSDKFLTVRLCLLVVDKA
                     AQRVEMFEDI"
BASE COUNT         1040 a          989 c          815 g          918 t
ORIGIN      
        1 tgcaaaaaaa ttgtctcagc aaattggcgc acaagctccg tacagacaag cacttttgtg
       61 ttttgatata cgctctgtcc ttttttatta ttaaagatac atgaatcttt tattcaaaga
      121 aagttcgccc tacttgaaac aacacgtcaa taaccccgtc cattggcaac cgtggggcga
      181 agcggcgttt gaagaagcca aacgccgcga cgttcctgtc ttcctctcca tcggttattc
      241 cacctgccac tggtgccatg tgatggcgca cgaatcgttt gaagacaacg aggtcgcatc
      301 tattctcaac gagcattttg tttcggttaa agtcgaccgc gaagagcgac ccgacatcga
      361 ccaagtgtat atgcacattt gctacgcaat gaccggccaa ggcggatggc cactcacatt
      421 cgttttattc ccagacaagc gccctttttt tgcaggcacg tacttaacaa agacgtcaat
      481 gtatggccgc ctagggctta tcgaacttct aaaaaatgtt acggctgttt ggagaaacca
      541 acgcagcgca ctcgaagccg aagcgagcga agcgttggga gagatccgtg gaaaaaaatt
      601 agaagttgca ggtaaggcag aactgtcgca catcaaaaac gccgtcgcat tttttgaaaa
      661 acgctttgac gcaaagcacg gcggatttag taaaaaaaca aaattcccct cgttccataa
      721 tttaattttc ctcctgcgtt actcccactt ctataatcat gaagccgcgc tcaatatggt
      781 tgaaaaaact atctacgaaa ttagcgccgg cggcatttat gaccaaatag gttttggtat
      841 acatcgttac tccaccgagc caacgtggca tttaccgcat tttgaaaaaa tgctctacga
      901 tcaggcgctg tatatcttca ctcttgccga gctttatcaa aagacaaagc gcgaatttta
      961 ccgcacccaa cttttgcgca cggttcaatt tgttgagacc acgctcgcat gccccgaacg
     1021 gggatattat accgcgctcg acgccgacac cgaacacgaa gaaggcaagt attatatttt
     1081 tacgtaccat gaactcagag aaattttgac cgatgaagaa cttcacttta tcgtgcgcca
     1141 tcttaccatc caggagcgtg gcaactttca cgatgaatcc accggcgaag aagtgaacgc
     1201 gaacattttt cattggcgcg aaaatccaac cggtggtgtt tcgctcaact gggatgcgct
     1261 tccacttttt gaacagtttg aacccatacg ccaaaaaatt ttatctgcac ggaaaaaacg
     1321 cactctcccc caccttgaca ataaaattct caccgacacc aacgctcttt acttagctgc
     1381 gctcgccaaa gccgcgttcg tgctcaacga tgtagcgtta aagaaaaccg cgcaaagcct
     1441 cgcacacttt cttttagcgc gcattgaaaa tggaacccta tggcatgtga aaaataacca
     1501 cggcaaaatc gacggtacac tcgacgacta cgcgtatacg gcgtgggggc tttgggagta
     1561 ttattccctc tcgcgcgaaa gtgcatcgct tctcgcagcg acttcactca ccgacactct
     1621 acttggaaat ttcgcagcag acggcggttt ttatttcacc gacaataaaa aggaaaccga
     1681 gtttgtcatc cgcacgcgcg aattctttga cggcgcactc cccacaggca acgcggtagc
     1741 gttagataac ctctggcggc tttttcaaac cacgaatgaa aataaatacc gcacagcgta
     1801 cgacaaacta ttggcgcttt ttggtggttt taccgccgaa attcccaccg catgcgcaca
     1861 cgcactcggt gtttttatga aaactctcgt gagcaaagag ctcgttttta ctcaggagag
     1921 cgctgcgttg gacgcagcgc tggcatccca atattcgcct tcgcttgctc tcgtcaaagc
     1981 aacgcccgaa cttgcagcac aatttcctta tctaaacgca tatcaaagcg atagcgaaaa
     2041 atactggctt tgccgtaact ttgcgtgcga agcaccagtg gcgagcgtgg ataaaatttt
     2101 tccacaactc taacaggatt tttaaactaa aatgcttgcc gcgcacaccc ccacattcaa
     2161 tacgaagtat tatggcactc gatgatcacg caggactcga agacattgaa gttttgaaat
     2221 cctcaatctg tgaattaaac caaaaagaac tccgctttcg ggggtacagc atcaacgatc
     2281 tggcagagaa agcaactttt gaagaggtgg tgtatttact atggaacgat gcactccctt
     2341 cgcgccaaga gctctccgac tttaccaacg cgttgcgtgc agaatacaat ttgccgcgtg
     2401 aaattatcga acacatcaac cacgttccac gcgaggcgca cccgatggcg gtattacgca
     2461 cattagtttc ttctttgggg ctgtacgacg aagacgctgc aaaccgcagc gtaccggcgc
     2521 agcaaaaaaa agcaataaaa ctgatagcga agattccatt gattgtagcg gcaatcgcac
     2581 gccaccgcaa cgccaaagcc aatgtcgaag ccaaccccga gttgggcatc gcggccaatt
     2641 ttctctactc gctcaccagc gccatccccg acacaaaaac agcgcgcctt atcgaaacga
     2701 cgctcattct ttacgccgag cacgaactca acgcgtcaac attcgccgcg cgcgtcaccg
     2761 ctgccaccga agccgacacg tactcgtgca tcgtttcggc gttgagtaca ttgcaggggg
     2821 ggctccacgg cggagcaaac caacgctcga ttgaaatgct cctcacgatt gcaaaccctg
     2881 aaagcgtcga tgaatacatc gcgccgcgtt tgaccaaaaa agaaatcatc atgggttttg
     2941 gtcaccgtgt ttacaaaaac ggcgatcctc gggtaccaat tttacgcact ctctgcgaga
     3001 gcctcgcgaa aagtcaaaac gaaaaaaaac tttttcagac ggcaaaacaa ctggaagaag
     3061 aaatgcttaa acgcaaaagc ttgcatgcaa acgtcgattt ttattcaggt ttagcttttt
     3121 atttactggg gctttctgcg gatattttta cctgcgtttt tgcgatggcg cgctctgctg
     3181 gctatttagc gcacatcgcc gaacaatggc aacgcaataa actcatccga ccgcgcgcag
     3241 tttacgaagg aaagaagaat ttggcgtttg ttcccatcga caaacggtaa aaattaaata
     3301 tcttcaaaca tttcaacccg ctgcgccgcc ttatcgacaa cgagtaggca aagacgcacc
     3361 gttaaaaatt tatccgactt ttcttgcatc cgttgcgcgg cctttctcaa cctgcttaat
     3421 tgtttttgac taatgcgcgg gtatgcaagc ccacgctgtc gtgccacttt ttttacctcg
     3481 aaaatataat acgcttcgtc ttctttgcgc gtgcacagca aatcgatttc cgcaccgtag
     3541 ctgcggttgt tgcggttgag cacaataaac cctttctcgc gcaaaaattg cgcagcgata
     3601 ttttcttctt cgttggtgaa gacttgctgc acgctatgtc tcaatcacag atgattattg
     3661 gtcaagacgg tgacgatgtt aacaaacctc aagcgcatgg gggtgttgac ttcaactata
     3721 aaagctcaat ctgcgctgat aagtccggca gcgcggagat cg
//
LOCUS       DAOMPD010000258         3753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10105_length_3753_cov_2.633856, whole genome shotgun sequence.
ACCESSION   DAOMPD010000258 DAOMPD010000000
VERSION     DAOMPD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10105_length_3753_cov_2.633856"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /gene="argB"
                     /locus_tag="PLY93_09280"
     CDS             complement(<1..308)
                     /gene="argB"
                     /locus_tag="PLY93_09280"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRP69708.1"
                     /translation="MDPTHAPSGERIETLLEALPYIQRFAGKTIVVKYGGAAMDKADL
                     SAKFAQDIVLLKLCGIDPVIVHGGGPQINQLLDRLGVKSTFIDGHRVTGDEEMATVEM
                     V"
     gene            complement(333..674)
                     /locus_tag="PLY93_09285"
     CDS             complement(333..674)
                     /locus_tag="PLY93_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRP69709.1"
                     /translation="MVFLIGMPNLGEMIFLVVIILLLFGGKKLPELARGLGAGIHEFR
                     RGMNGQNEPTETRVHDVERDVTPTQEEAAVQQKQITAPRKGVRAAAPTKKKTTASKTV
                     AKKAPAKSKKK"
     gene            770..1234
                     /locus_tag="PLY93_09290"
     CDS             770..1234
                     /locus_tag="PLY93_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69710.1"
                     /translation="MHRWIYPVSSVFLVLVLLLSCSKKNENSVDGNSTVEGMPPKAPE
                     SAAPEYMQRLGLLYPQAYYYRVGKNFLQKTNHPLAEILRYYETALEKNNFKIIARLSQ
                     NDGALLQGEKKVKAFQEVLSIDVQKLPYADNFLIRVGRSQVDYASLEKRGEP"
     gene            1231..2361
                     /locus_tag="PLY93_09295"
     CDS             1231..2361
                     /locus_tag="PLY93_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRP69711.1"
                     /translation="MKLDRSYSFLDFSRFLPFAFFAIFLSLLTLVIFVYYSYIYAFFI
                     SGILFILFRSPHRWLLKQLHGRRNLAAVISTFLVILILVIPSTFLVLALVGEAKHAIE
                     MARQWFTPEKINEIYQNNLWLQDRFNLRIEEIAAYRAKLFDVILANQFETIKQGWQWI
                     LTSIKFFVDFTFALFILFFLFRSVDHIGQVVYRSLPFPDKFEKYIGERMVKIFDTVVK
                     GNLIVSILQGSVIGIVFWFCGLSTPLLWGAIAVPFALIPVIGTAVVWLPGAVYLYSQG
                     MHTTSFIMGAGCVLAYFLLENLIKPMLLDRDLNLHPLFLFLAIVGGLNAFGIKGLILG
                     PFIVTMFVTIWQLVSEWNGSYVGQNTPLESDEYEKSDEGRSP"
     gene            2364..3185
                     /locus_tag="PLY93_09300"
     CDS             2364..3185
                     /locus_tag="PLY93_09300"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRP69712.1"
                     /translation="MPARQKILILGVLLITLGAVLTTWFFVKRHAGGDGWRITVEGNR
                     IVTEKEIQEVVEYLLKAAKDGVSADEIRDTLLLNPRIQTAKVSVLPAQKIHVTVTERL
                     VAYLQNEQEGIAEKDAAGVTIVENVATLNKDLTPDKVIFYLTFRNESPESPRSDIIRL
                     WQKTHEKYAFLWQRFAEIEIRPLRRGTDTSGQEAGEGKGNDNHLWRYRIYSAGVRSCI
                     VYEGRFSEETLRRLWAVYAYLETKLPRSVTLVDLQENSAIIREMKNIHHNNEVGS"
     gene            3182..>3753
                     /gene="ftsA"
                     /locus_tag="PLY93_09305"
     CDS             3182..>3753
                     /gene="ftsA"
                     /locus_tag="PLY93_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRP69713.1"
                     /translation="MSYDGAPMLAGRLIAALDLGSSKTVAIVARVASPDEVEVIGLGV
                     VESRGVKSGSVTNIEHTVRSIQEAIEEAELMSAVEIDEVIINISGKNVRGDNATGMVT
                     ITGRDRIITHADVFRVIDTAQNIRIPADQEILHVLSREFKVDDQAGIRDPLGMVGVRL
                     DAQVHIVTAPVTQLQNTEKAVEQAGVRVSN"
BASE COUNT          924 a          839 c          918 g         1072 t
ORIGIN      
        1 accatttcaa cggttgccat ctcttcgtcg ccggtgacgc ggtggccgtc gatgaatgtg
       61 gacttgactc ccaatcggtc taagagttga ttgatttgcg ggccgccgcc gtggacaatc
      121 accgggtcga tgccgcagag ttttaagagg acaatgtctt gcgcaaattt tgcgctgagg
      181 tcggctttat ccatggcggc gccgccgtat ttaacgacga tggttttgcc ggcaaagcgt
      241 tggatataag gcaacgcctc gaggagcgtt tcgatacgct cgcccgaagg cgcatgggtt
      301 ggatccacgg taatcacggt tgaaaaagtg cgttactttt tcttggattt tgccggtgct
      361 tttttggcca ccgttttaga agcggttgtt tttttctttg ttggtgctgc agcgcgcacg
      421 cctttgcggg gggcagtgat ttgtttttgt tgtactgccg cttcttcttg cgttggcgtg
      481 acatcgcgtt cgacatcgtg tacccgagtt tctgttggtt cgttttggcc attcatgcct
      541 cgacgaaatt catggatgcc tgcccctaaa ccacgcgcca gttcgggtaa tttttttcca
      601 ccgaaaagaa gaaggataat caccaccaag aaaatcattt cgcctaaatt aggcatgcca
      661 attaagaaga ccatgacgaa gtaaaagtga taattgacac cctgtaaagt aaaatacagt
      721 tcttgtcaat ttacgctgta cgcgcgtgat tcaataatta tgccgcgcca tgcacagatg
      781 gatttatccc gtttcgagtg tctttttggt gttggtgttg ttgctttctt gcagcaagaa
      841 aaatgaaaat agtgtcgacg gaaattccac cgtagaaggc atgccaccga aggcgccaga
      901 aagtgccgcg cccgagtata tgcagcgctt ggggctgctt tacccacaag catattacta
      961 ccgtgtgggt aaaaattttt tacaaaaaac aaaccatccg ttagcggaga ttttgcgcta
     1021 ttatgaaact gctctggaga aaaataattt taagataatt gcgcgtttat cccaaaacga
     1081 cggcgcgttg ttgcaaggtg aaaaaaaagt aaaggctttt caagaagtat tgtcgatcga
     1141 tgtgcaaaaa cttccatacg ccgataattt tctcatccgt gttggccgtt cgcaggtcga
     1201 ttacgcttcg ctcgagaaaa gaggcgagcc atgaaacttg atcgcagtta tagctttctc
     1261 gatttttctc gatttttacc gtttgctttt tttgcaattt ttctttcttt gctaacgctt
     1321 gttatttttg tttactacag ttatatctac gcgtttttta tttcaggtat tttatttatt
     1381 ttgtttcgct cacctcaccg ttggttgcta aaacaattac acggaagacg caatttggca
     1441 gcggtgattt cgacgttctt agttatcttg atactcgtta taccgagcac ttttttggta
     1501 ttggcgcttg tcggtgaagc aaagcacgcc attgagatgg caaggcagtg gttcactcct
     1561 gagaaaatca atgaaattta tcaaaataat ttgtggcttc aagaccgttt taacttgcgt
     1621 attgaagaaa ttgccgctta tcgtgcgaag ttgtttgacg ttattttagc caaccagttc
     1681 gagacgatta aacagggctg gcaatggata ctcaccagta tcaaattttt tgtagatttt
     1741 acatttgcac tttttattct cttctttctt ttccgcagtg tcgatcacat cgggcaagtg
     1801 gtttaccgca gcttaccttt tcccgataag tttgaaaaat atatcggcga acggatggtg
     1861 aaaatttttg acactgtcgt taaaggaaac cttattgttt ctatactgca aggttcggtg
     1921 attggtattg ttttttggtt ttgcggactt tcaaccccac ttctctgggg agccatcgct
     1981 gtgccctttg cgctcatacc ggtgattggc acggccgtgg tgtggctccc gggagcggta
     2041 tatctctatt cacaggggat gcacacgacg tcattcatta tgggggcggg gtgtgttctc
     2101 gcctattttc ttctcgaaaa tcttattaag cccatgttgc tcgaccgcga tttaaacttg
     2161 cacccattgt ttctttttct cgctattgtc ggtggattaa atgcgtttgg tatcaaagga
     2221 cttattttag gaccttttat tgtaacaatg tttgtcacca tttggcaact tgtgagtgaa
     2281 tggaacggta gctatgtagg tcagaacact cctctggaga gtgatgaata tgagaagagc
     2341 gacgaggggc gctctccata aaaatgcctg cccgtcaaaa aattctcatc ctgggcgttt
     2401 tactcatcac tttaggtgcg gttctcacaa cgtggttttt cgtgaagcgg cacgcaggcg
     2461 gtgacggttg gcgtatcact gtggaaggta accgtatcgt cactgaaaaa gaaattcagg
     2521 aagtggtgga gtatttactc aaagccgcta aagacggtgt gagtgccgat gagattcggg
     2581 atacattgct tttaaacccg cgcattcaaa cagcgaaggt gtcggtgtta ccggcgcaga
     2641 aaatccatgt caccgtcaca gaacggttgg ttgcatattt acaaaatgaa caagaaggca
     2701 tcgccgaaaa agacgcggca ggagtcacca tcgttgaaaa tgtggctacg ctcaacaaag
     2761 acctcacccc cgacaaagtc attttctact tgacttttcg caacgaatca ccggaatcac
     2821 caagaagtga cataatacgg ctttggcaaa aaacgcacga gaaatacgct tttctttggc
     2881 aacgttttgc tgaaattgaa atccgccctc ttcgaagggg gacggacacg agtggtcaag
     2941 aagctggcga agggaaagga aacgataacc acctttggcg ttatcgtatc tattcggcgg
     3001 gggttcgctc gtgtattgtt tacgaagggc gtttttcaga agaaaccttg cgccgccttt
     3061 gggcagtgta cgcgtatctt gaaacaaaac tgccgcgttc tgtcactttg gtagacttac
     3121 aggaaaacag cgctattata cgcgaaatga aaaatatcca ccacaacaac gaggtcggct
     3181 catgagttac gacggcgcgc cgatgctcgc agggcggctt atcgccgcat tagatttagg
     3241 ctcttccaaa accgtcgcaa tcgtcgcccg cgttgcttca cccgatgagg ttgaagtcat
     3301 cggtttgggc gtcgtcgaat cgcgcggcgt caaatcgggt tcggtgacaa acatcgaaca
     3361 caccgtccgc agcatccaag aagccatcga agaagccgag ctcatgtcgg cggttgagat
     3421 tgacgaagtc attatcaata tttctggtaa gaacgtacgc ggcgacaacg ccactggcat
     3481 ggtgacgatt accggtcgtg atcgcatcat cactcatgcg gatgtcttcc gtgttatcga
     3541 cactgctcag aacatccgca ttcctgccga tcaagaaatt ttacacgtcc tttcgcgtga
     3601 atttaaagtc gatgaccaag ctggcatccg cgatccactc ggcatggttg gtgtacgttt
     3661 ggacgcgcaa gtacacattg tcactgcacc ggtgacccaa ctccagaaca cagaaaaagc
     3721 tgtagagcag gccggtgtgc gcgtctccaa taa
//
LOCUS       DAOMPD010000259         3749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10117_length_3749_cov_3.253384, whole genome shotgun sequence.
ACCESSION   DAOMPD010000259 DAOMPD010000000
VERSION     DAOMPD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10117_length_3749_cov_3.253384"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..730
                     /locus_tag="PLY93_09310"
     CDS             113..730
                     /locus_tag="PLY93_09310"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRP69714.1"
                     /translation="MQIFFLAVSLTVLPLFAGEGLEFMQNIINNFESLRTELSEDFSP
                     AKQKEVAHRARELALTILDVNEIGKLALGKHYEKLTAKQQKDFLGLFHELMANRVVEA
                     NIPSQKVISAKIPIEIISERVENDDVFDKEAIIVNTAVPHRKQNFKVEFYLYRTDSGL
                     KLYDVHVDEASTLLDFRNQFSSIIRKKGYKHLVKLLRERIAKINK"
     gene            711..2048
                     /locus_tag="PLY93_09315"
     CDS             711..2048
                     /locus_tag="PLY93_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP69715.1"
                     /translation="MQKLINSVALFSIFGGAFLYAEKPIRFADGLTQLLKNNANILES
                     KAQILSFAAYQSEASALDYPTFSLLTYAAPIFESKGTPQSSKVDYAKWGPYVHAELQI
                     VYPIFSFGRIKDGKKAADHAVAGARYLHESQINKSIFEYKQLYLQIILLNRLKKVLDD
                     ANEKMGAVLSQAEKLYKKGTGDVQRKDLTRLKLFALELQRFNAEWLANKKTSALALGH
                     FLGERESLIVLEQDFPHVDRSTRALEQLIAEAHEANPDLKALTEGLQARHHQLEMEKG
                     GHLPVLFLASRSEVNYANTRSEQQSSYAFDPYNRDWFAVVIGAKWELDFGNTKAKIQR
                     AEAEYEKLKAKESEALTGIPLKIAMAMWDFEKQRAQLEISQKKYKEANKWSLSEMTGY
                     SAGTGNAKDLIEAFGAQLLTEKEVAEGEFNLCLAAAKLAMEVGDQTTLKTWQE"
     gene            2056..3618
                     /locus_tag="PLY93_09320"
     CDS             2056..3618
                     /locus_tag="PLY93_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP69716.1"
                     /translation="MKFIALILLPLLFATLLFAKRRELLLGEKAYSHGAYDTARKHFE
                     AAIENGEASGDPHLYIGLILESRRQYAESIPYFRAAAERPMTKKFKKVAYWKMVILCR
                     KAKQYAEALRYVERLESMGEKSELFEKIRAEADGHSITSPEDRSLKGFATIQKATNLE
                     EDLRKESGQGQDETKIQNLRKEIIATYQKAIEENPYWKEYRWKIARHYERLEDREGAL
                     ATYRAIWEDTANPRAAYKLGIGAKRVSNYTQALKYFGAALEKVGNDNTLGFYVRLNAA
                     QSHYALTHPVEALAHAKIAKKYAEEIQLSEKTQASLKRVFCLAAISNKEDDAKYCKFS
                     PKTESAGFINLIEMKRALAQKNNTTAAKFATRIYENDAEEVDEGEAETAIPAYAYSDL
                     PLAIEVLFQTENYRNVLELTDKFEEKLTYYADLHTWRAISHFALKEYPLAVLEFNKIK
                     KLSPVQMNLHLMSLAHTGNWAEVKSKGALYMKDTRAREKLEKNFRTLKLYAPLREEAD
                     FEIWLRGVSVDN"
BASE COUNT         1142 a          830 c          889 g          888 t
ORIGIN      
        1 ttttggtcgg tactttttta agtgttgcaa aagtgattct tgtggtgttg gcaatcatca
       61 taattgttgc ctatttccgt aaaaaaagct aattttgaac tgaatgcgtt ttttgcagat
      121 tttttttctc gctgtttctc taacggtgct gccgcttttt gcgggcgaag ggttagagtt
      181 catgcagaac atcatcaaca attttgaatc gctgcgtacc gaactctccg aagatttctc
      241 gcctgccaaa caaaaagaag tggcgcatcg tgcgcgcgaa ctagcgctca ccattttaga
      301 tgtgaatgaa attggcaagt tggcgttagg gaagcactac gaaaaactta ccgccaagca
      361 acaaaaagat ttcttgggat tgttccacga actcatggcc aaccgcgtgg tcgaggcgaa
      421 catcccttct caaaaagtga ttagcgcaaa aataccgatt gaaatcattt cggaacgcgt
      481 tgaaaacgac gacgtctttg ataaagaagc gattatcgtc aacactgccg tgccgcaccg
      541 caaacaaaat ttcaaagtcg agttttatct ctaccgcacc gacagcgggt taaaactcta
      601 cgacgtgcac gtcgacgaag cgtcgacgct gcttgacttt agaaaccagt tttcatcgat
      661 tatacgaaaa aaaggctata aacaccttgt gaaattatta cgtgaacgga ttgcaaaaat
      721 taataaatag cgttgctcta ttctctattt ttggcggagc attcctttac gctgaaaaac
      781 cgatccgctt tgccgacgga ttaacacagc tacttaaaaa caacgccaac attttagaga
      841 gcaaagcgca aattttaagt tttgccgcgt accaaagcga agcgtcggcg ttggattatc
      901 cgacattttc tttactcacg tatgcagcgc cgatttttga atctaagggg actccgcagt
      961 cgtcgaaagt tgattatgca aagtgggggc cttatgtcca cgccgagttg caaattgtgt
     1021 accccatttt tagttttggc cgcatcaaag acggtaaaaa agccgcagac cacgctgttg
     1081 cgggcgcacg ttatttacac gaaagtcaaa tcaataaaag tatttttgag tataaacaac
     1141 tttacttgca aattattttg ctcaaccgcc tcaaaaaagt tttagacgat gcgaacgaaa
     1201 aaatgggcgc agtgctttct caagcagaaa aattatataa gaaaggcacc ggtgacgtac
     1261 agcgcaaaga tttaacgcgc ttgaagttgt ttgcgttaga gttgcagcgg tttaacgcag
     1321 aatggttggc caataaaaaa acgtcggcgt tggcgctggg gcatttttta ggagaaaggg
     1381 aatcgctcat tgtgttagag caagattttc cgcacgtcga tcgctcgacc cgcgcattgg
     1441 aacaacttat cgccgaagcg catgaagcca accccgatct aaaagcgctc accgagggat
     1501 tgcaagcgcg ccaccaccaa ctcgaaatgg aaaaaggcgg tcatttgccg gtgctttttc
     1561 ttgccagccg ctccgaggtg aattatgcga acacccgctc cgaacaacaa tcgagctatg
     1621 cgttcgatcc atataaccgc gattggtttg ccgtggtcat cggcgctaaa tgggaactcg
     1681 attttggtaa tacaaaagca aaaatccaac gcgccgaagc cgagtacgag aaattaaaag
     1741 ccaaagaaag cgaagcgtta acaggtattc cgcttaaaat cgcaatggcg atgtgggatt
     1801 ttgaaaaaca acgcgcacaa ttagaaatca gtcaaaaaaa atataaagaa gctaacaagt
     1861 ggtcattgtc cgaaatgacc ggctatagcg caggcaccgg taatgcgaaa gatttaatcg
     1921 aggcgttcgg cgcacagtta ctcactgaaa aagaagtcgc ggaaggtgag tttaatctat
     1981 gccttgccgc cgcaaaatta gccatggaag tgggcgacca gacaacactc aaaacatggc
     2041 aggagtaaac gccgtatgaa gttcatcgcc cttattttat tacctctcct ttttgcgacg
     2101 ctactttttg caaaaaggcg agagttgtta ctcggcgaaa aagcatattc ccatggtgcg
     2161 tacgataccg cgcgcaagca ttttgaagct gcaattgaaa atggcgaagc cagtggcgat
     2221 ccacatttat acattggatt gattttggaa tcgcgccgcc aatacgcaga aagcattccg
     2281 tattttcgtg cggcagctga acgccccatg acgaagaaat ttaaaaaagt tgcgtactgg
     2341 aagatggtta tcttgtgccg caaagcaaag cagtatgccg aggcgctgcg ctacgtcgag
     2401 cgtctggaat cgatgggtga aaagagcgaa ctctttgaaa aaatacgcgc agaggcagac
     2461 ggtcattcga taacatcccc cgaagatcgt tcgttgaagg gatttgccac cattcaaaaa
     2521 gccacaaatt tggaagaaga cttgcggaaa gagagtggac agggacaaga tgaaacaaaa
     2581 attcaaaatt taagaaaaga aattatcgcg acctatcaaa aagcgattga agaaaatccc
     2641 tattggaaag aataccgttg gaaaattgcg cgccactacg agcggttaga agatcgagaa
     2701 ggggcgctcg ccacctaccg cgccatttgg gaagacaccg ccaacccccg tgcggcgtac
     2761 aaactgggca tcggcgcaaa acgagtgagt aattacacac aggcactgaa gtatttcggg
     2821 gcggcgttag agaaagtggg caacgacaac acgttggggt tttacgttcg acttaacgcg
     2881 gcacaatcgc actacgcgtt gacgcatcct gtcgaagcat tggcgcacgc aaagattgct
     2941 aaaaaatatg ctgaagaaat tcaactctct gaaaaaacac aggcgagtct gaaacgcgtg
     3001 ttttgtttgg cggctatatc caacaaggaa gacgatgcca agtattgcaa gttctctcct
     3061 aaaacagaaa gcgcagggtt tatcaacctc atcgagatga aacgcgcgct tgcccaaaaa
     3121 aataatacca ctgctgcaaa atttgccacc cgcatttatg aaaacgacgc cgaagaggtc
     3181 gacgaaggcg aggcagagac ggcgattccc gcatatgcgt actccgattt accgctagcg
     3241 attgaagtgc ttttccaaac ggaaaattat cgcaatgtac ttgaacttac cgacaagttt
     3301 gaagaaaagc ttacttacta cgccgacttg cacacatggc gtgcaatctc gcactttgcg
     3361 ctcaaagagt acccattggc ggttttagaa tttaacaaaa ttaaaaaatt gagtcctgta
     3421 caaatgaact tgcatctcat gtcgctggcg cacaccggta attgggctga ggtaaaaagt
     3481 aagggcgctc tttatatgaa agatacgcgt gcgcgagaaa agctcgaaaa aaatttccgc
     3541 actttaaaat tgtatgcgcc gctgcgagaa gaggccgatt ttgaaatctg gctgcgtggt
     3601 gtatcggtcg ataattaagc gggcagattg tctaacccac ggacatacct gacaaatgct
     3661 acgccgcaaa tcagcgacga tagagggcgg taagccgtct tgttctgaaa ttcaatggcc
     3721 aataatcggt ctgaatggcc atattgggc
//
LOCUS       DAOMPD010000260         3746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10125_length_3746_cov_2.543755, whole genome shotgun sequence.
ACCESSION   DAOMPD010000260 DAOMPD010000000
VERSION     DAOMPD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_3746_cov_2.543755"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLY93_09325"
     CDS             complement(<1..251)
                     /locus_tag="PLY93_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRP69717.1"
                     /translation="MARRVAVISSGQTFHSGGRADVNGQELITEAVTRALKASDLTID
                     AIDAIVIGNMDHFEGINYVDSWSAPGTGGTMKPVIKLTTG"
     gene            complement(319..1476)
                     /locus_tag="PLY93_09330"
     CDS             complement(319..1476)
                     /locus_tag="PLY93_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRP69718.1"
                     /translation="MEKIAIIGVGQTKQERNKKNQNFAEMVYEATQHALADAGIEIKD
                     IDNVITVSNDFWDGRTISSMAVQDAAGAFEKNVSTVEGDGTFGAYYAMTRVLSGSYKN
                     TLVVAHSKGSESDTNLITNGMFEPIFTRPLGLDNITTAALQARAYMEAFSIKEEDFAA
                     VAVKNHANSLKNPNAQIAKTITISDVMQSKKYADPLKLYDISPITDGAAALILSGDTF
                     LQKLKGGKAKPVYVKGAAFCSDAYDLGLRDLARSPALQKAAATAYRMAGIKTPAEEIH
                     LAELYDAFSYMELMWYEGLAFCDEGKGADFIRSGKTQLTGALPVNASGGVLSAHAVIT
                     AGLIRMIEAALQIRGDAGAHQVGRKVETALAHGINGPCAQAHCVWVLGKEA"
     gene            complement(1525..1998)
                     /locus_tag="PLY93_09335"
     CDS             complement(1525..1998)
                     /locus_tag="PLY93_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRP69719.1"
                     /translation="MSDWRENVEPIIHPSKIKVPYTWSVGETGSKFLRALRDKKKIIA
                     NKCPTTGQVFVPPKLNSPYSLEPITEWLELKGTGVIKSFTLQRYESAAHPQGESKIFA
                     LILLDGATQALPHFLGEIDFENVKMGMRVEAVFRENREGSILDIMYFKPTERDIS"
     gene            complement(2071..3282)
                     /locus_tag="PLY93_09340"
     CDS             complement(2071..3282)
                     /locus_tag="PLY93_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP69720.1"
                     /translation="MPQRLESTAWEPVHIFELWRGEWGLTREAEFRADPWYIVSGKEV
                     VDSGFGKPPKDFFSKTLPPGVVVPGFVNAHSHIELSCMLGKVPVGSHLTQMGTIIQAQ
                     RGSLTKQQIRQAAVTAIRHAENFGTFFFCDICNSVDFIRFLRDLPLFNGNRYLELLGF
                     SSPSDQARIASAQELLDIDSTFYPTVHSIYGSSPLQMRFVREKARHTSVSIHLLESAA
                     EIDFQDDKGQIVEFLEQIGQRVRHHEMQGRSAIDYLYAMGMLSFKKLLMVHLSAARVE
                     DIDKLNEYVPHAAWVLCHRSNKHLVIERTNWEKFKNSPLKMLIGTDSLATNTDLSVLH
                     ELKAILQEDKFSERDLLRAATWDAYEYLEFRNNTIPHFIYPNARPNIASLGSVARVEV
                     LGVQNNHPETR"
     gene            3335..>3746
                     /locus_tag="PLY93_09345"
     CDS             3335..>3746
                     /locus_tag="PLY93_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRP69721.1"
                     /translation="MLLTLKGTYTALITPFTPDGERVDWGALKKLVETQIDAGVEGLV
                     PCGTTGESPTLSPAEHSEVIAKVIEWAKAKKSSTVVIAGTGSNSTAEAIHLTKEAAAA
                     GADAVLSVNPYYNKPVQRGLVQHFQRIADASKVPV"
BASE COUNT          873 a          916 c          967 g          990 t
ORIGIN      
        1 ccagtggtga gtttaatcac cggtttcatt gtgccgccgg taccgggggc agaccacgaa
       61 tcgacgtagt taatcccttc aaagtgatcc atgttaccga tgacgatggc gtcgatggcg
      121 tcgatggtga gatcacttgc tttaagcgca cgggtgacag cttcggtgat gagttcttgg
      181 ccgttcacgt cagcacgccc accgctgtgg aatgtttggc cggagctaat gactgctacg
      241 cggcgtgcca ttacgttacc aactccagcg cttcgttgag ataatctcgc attgtttgca
      301 tagcttctta ttccacagtt atgcctcttt tcctaaaacc cacacgcaat gcgcttgtgc
      361 gcaagggccg ttgatgccat gcgcgagagc ggtctcgact tttcgtccca cttgatgcgc
      421 ccccgcatcg ccacgtattt ggagcgccgc ttcaatcatg cggataagac cggcggtgat
      481 cacagcatgc gcggacagca caccgcccga tgcgttcacg gggagtgcgc ccgttagttg
      541 cgttttaccg ctacggatga aatccgcgcc tttaccttcg tcgcaaaagg ccaaaccttc
      601 gtaccacatg agctccatat aactaaacgc atcgtatagc tcggcgaggt ggatttcctc
      661 tgcaggcgtt ttgatgccag ccatgcgata ggcagttgca gccgcctttt ggagtgcggg
      721 tgagcgtgct aagtcccgta aacctaagtc gtacgcatcg gagcagaacg ccgcgccttt
      781 gacatacacc ggttttgctt tgccgccttt aagtttttgt agaaatgtat cgcctgacaa
      841 aatgagcgct gccgcgccgt cggtgatggg ggagatgtcg tatagtttca atgggtctgc
      901 gtattttttg gattgcatca cgtcagaaat tgtaattgtt ttggcaattt gtgcgttggg
      961 atttttgagc gagtttgcgt ggttctttac tgcaaccgca gcaaaatctt cttcttttat
     1021 tgaaaacgcc tccatgtacg ctcgtgcttg cagcgccgca gtagtgatat tgtcgagacc
     1081 taaggggcgc gtgaagatgg gttcaaacat accgttggtg atgaggtttg tgtcgctttc
     1141 ggagccttta ctgtgcgcca cgacgagtgt atttttatac gagcccgaaa gaacacgcgt
     1201 catggcgtag tacgcaccaa aagtgccgtc gccttccact gtcgaaacat ttttttcaaa
     1261 cgctcccgca gcgtcttgca ctgccatcga cgagatggtg cgaccatccc aaaaatcgtt
     1321 cgagacggtg atgacgttgt cgatgtcttt tatctcaatg ccggcatcgg cgagcgcgtg
     1381 ctgcgttgct tcgtacacca tctcggcaaa attctgattt tttttattgc gttcttgttt
     1441 ggtctgacca acccctataa ttgcgatttt ttccatgtta ttcctgttta tttattattg
     1501 ttgtcgtaga gacgcaccat ggtgttagga tatatccctt tctgtgggtt tgaaatacat
     1561 tatatctaaa atgcttcctt cacggttttc gcggaaaacc gcttcgacac gcattcccat
     1621 tttcacattt tcaaaatcga tctctcctaa aaaatgcggc agcgcttgtg tggcgccatc
     1681 gagcaatatg agcgcaaaaa ttttggattc accttgagga tgcgctgcgc tttcatagcg
     1741 ttgcaaggta aacgatttta tcacacccgt gcctttgagt tcaagccatt cggtaatggg
     1801 ctcgagcgaa tagggggagt tcaatttggg cggaacaaac acttggccgg tggtggggca
     1861 tttgttcgcg atgatttttt ttttatcgcg gagcgcacgc aaaaactttg agcccgtctc
     1921 gcccaccgac cacgtatatg ggacttttat tttactgggg tgtataatcg gttctacatt
     1981 ctcacgccaa tcgctcatgc ggacacatta gagaaaagga gatacgaggg gaaataaaaa
     2041 gagtattagt attatgggcg atggatgtct tcagcgagtt tcgggatggt tattttgaac
     2101 acccaaaact tcgacgcgcg cgacactgcc gagcgaagca atgttggggc gcgcattggg
     2161 gtaaataaaa tgcgggattg tgttgttgcg gaactccaag tattcgtacg catcccacgt
     2221 tgcggcacgt agtaaatcgc gttctgaaaa tttatcctct tgtaaaatcg ctttaagttc
     2281 gtgcaacacc gataaatcgg tatttgtcgc gagtgagtcg gtgccgatga gcattttgag
     2341 cggtgagttt ttgaattttt cccagttagt gcgttcgatg accaagtgtt tattcgagcg
     2401 gtggcaaagc acccatgccg cgtgcggcac atactcgttc aatttatcga tatcttcgac
     2461 gcgtgccgcc gataaatgca ccatcaaaag ttttttgaaa gagagcatcc ccatcgcgta
     2521 gagataatcg attgccgaac ggccttgcat ttcatggtgg cggacgcgct gcccaatttg
     2581 ttctaaaaat tcgacaattt gtcctttgtc gtcttggaaa tcaatttcgg cggcactctc
     2641 taaaagatgg atagagaccg atgtgtgccg cgctttttca cgcacgaagc gcatttgcaa
     2701 tggggacgat ccgtaaatcg aatgcaccgt tggatagaat gtggaatcga tatctaaaag
     2761 ttcttgggcg ctcgcaatgc gtgcttgatc gctgggagag gaaaatccca agagctctaa
     2821 atatcgattg ccgttgaaca acggtaaatc gcgcaagaag cggatgaagt cgacgctgtt
     2881 gcagatatcg caaaaaaaga acgtaccaaa attttcggcg tggcggatgg cagtcactgc
     2941 cgcttgccgt atttgttgtt tagttaatga accgcgttgc gcctgaatga tcgtacccat
     3001 ttgagtcagg tgactaccca ccggcacttt gccgagcatg cacgacagct cgatgtggct
     3061 atgtgcattc acaaacccag gcaccacaac gccgggtggt agtgttttgc taaaaaaatc
     3121 tttgggtggt ttgccgaaac cagagtcgac gacttctttg ccggaaacaa tataccacgg
     3181 gtcggcacgg aattcagcct cccgtgttaa cccccactcg ccgcgccata gctcaaaaat
     3241 atgcacaggt tcccaagcgg tactttcgag tcgttgcggc aaccataaca cgtttagttt
     3301 ttgacgccct gtcttttaca cggctgcggg aagtatgctc ttaacactca aaggaacgta
     3361 cactgcgctc atcactccat ttactcccga cggcgagcgt gtcgattggg gtgcgcttaa
     3421 aaagctcgtt gaaacgcaga tcgatgccgg tgtggaaggg cttgtgccat gcggcaccac
     3481 cggtgaatcg cccacgctct cacccgccga gcacagcgag gtgatcgcta aagtgattga
     3541 gtgggcgaaa gcgaaaaaat cgagcacggt tgtgattgca ggcacgggca gcaattcaac
     3601 agcagaagca atccacctta caaaagaagc tgccgctgca ggagccgacg cggtactttc
     3661 ggtaaacccc tattacaaca aaccagtgca gcgggggctc gtgcaacatt ttcagcggat
     3721 cgccgacgcc tcaaaagttc cagtgg
//
LOCUS       DAOMPD010000261         3719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10207_length_3719_cov_2.993450, whole genome shotgun sequence.
ACCESSION   DAOMPD010000261 DAOMPD010000000
VERSION     DAOMPD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10207_length_3719_cov_2.993450"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /gene="hemG"
                     /locus_tag="PLY93_09350"
     CDS             complement(<1..1285)
                     /gene="hemG"
                     /locus_tag="PLY93_09350"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRP69722.1"
                     /translation="MSRIAVVGGGISGLAHAYYWKKKHPKDEIILFEAGKLGGNIQTQ
                     THDGALLEPGPDSYLERSGLFTTMIRDLGIESELLHETKNTAKRYVVKDGYLVKAPTS
                     PGSLFFSSLLFFPEKIKMFLAMGKKFSIWPTITLFDAARNVLGFSTAEYLASPMARGI
                     YGSEAEDIEFSSIFPELFKKITETPKLKIALKEYSREKKEYWQNALGGVSFERGIYNF
                     KGGLITWVNALEKYLSANGVSIIHDKIARIQKHSDGKHALSSKAKSYNTFDKIIFTVG
                     RDSLAYIWREADKELSRKITEVKHSPINVVYAAWNKKDLYAGGYGFLVPRKERISILG
                     CIFASNVFPGRIKEDYFLTKTMVSGDGALFKDEDLSHLSQESLARVLRIKANPLWTKV
                     FRQTPGIPRYAPGYGELKREIFTLLEKHEGVHFAGW"
     gene            1371..2057
                     /locus_tag="PLY93_09355"
     CDS             1371..2057
                     /locus_tag="PLY93_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRP69723.1"
                     /translation="MLVAMVQEVATTAMGYAVIIKLEFKNKIIPVFVGPTEAFAISAI
                     LHKETRDRPIAVDLLATLITESKARLEKVFIHDFQGGTFLARIYLTGEQFNKGALELD
                     ARPSDALALALRLRSPVYVADHVYDRTAIKFSTVREADNKAIHTDSESIDDILSDDER
                     EEFFEAILEEFGEQKKAVKKTKPAVEKIKFLSRSEALERMLKKALADEQYEEAARIRD
                     ELRAESSKTN"
     gene            complement(2075..3109)
                     /locus_tag="PLY93_09360"
     CDS             complement(2075..3109)
                     /locus_tag="PLY93_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HRP69724.1"
                     /translation="MFSLFKKKEKPTFSAYIQSMNKTVTVKHRQTLLEAALEAGLDYP
                     FSCGAGSCTSCKTKLVQGEYKDLSDMSLVLTEDDLKKGYILACQTLPKTDLIVTLEPD
                     ESKEYAGTVSTYHKLTHDIIEVKIKLAEKMRFSAGQYADFAIPGIKGTRSYSFAAAPG
                     AGGASFEVVFFIRLVPGGQVTEWFHSKDRTGEAIKIRGPRGTFGWRDHKDTLLCIAGG
                     SGMAPIKAMLEDAAQKKLTKQIIYLFGARTQKDLYALDDMKKLAAQLKLTFTPILSHE
                     EEGSSWKGARGFVTELIAQQKIDFANTAAFLCGPPAMIDAAIQKLEECGVSKHEIFFD
                     KFTDKSHIKS"
     gene            3222..>3719
                     /locus_tag="PLY93_09365"
     CDS             3222..>3719
                     /locus_tag="PLY93_09365"
                     /inference="COORDINATES: protein motif:HMM:NF040252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HRP69725.1"
                     /translation="MDKHTMLSPTLNDNPLLHQGALPDFEKIRSEHVVPAVRAALTLA
                     EEALAAVEKEENPTWENFVLPLRQATRRLDQAWSAVNHLMGVKNSDDLRKAHDAVQNE
                     VVAFSLKMGQSRALYNAWEKLQKNKSELSTPARVRIVEAALRDARLSGIALEGKAKER
                     FNAIQQ"
BASE COUNT          992 a          850 c          893 g          984 t
ORIGIN      
        1 tccaccccgc aaaatgcaca ccttcgtgct tttcgaggag agtaaaaatt tcgcgtttta
       61 actcgccgta acccggcgcg tatcgaggga ttccgggcgt ttggcggaaa acttttgtcc
      121 ataaaggatt tgctttgatg cgtaacacgc gcgcgagaga ttcttgcgac aagtgggaaa
      181 ggtcttcgtc tttgaataat gccccgtcgc ccgaaaccat cgtcttggtg aggaagtaat
      241 cttctttaat tcgcccggga aaaacattcg aggcaaaaat acatcctaag atggaaatgc
      301 gttctttacg cggtactaaa aatccatagc caccggcgta taaatctttt ttattccaag
      361 cggcgtacac cacgttgatg ggtgaatgct tcacttcggt aattttacgc gacagctctt
      421 tatcggcttc gcgccaaatg tacgcaagac tgtcgcggcc tacagtgaaa ataattttat
      481 cgaaggtgtt atacgatttt gcttttgagg aaagcgcatg cttgccgtcg gagtgcttct
      541 gaatgcgcgc gattttgtcg tggataatgc tgacaccgtt tgcgctaaga tacttttcca
      601 gcgcatttac ccaagtgatg agaccgcctt tgaaattgta tataccgcgc tcaaacgaaa
      661 cgccacccaa tgcgttttgc cagtattctt ttttttcgcg cgagtattct ttgagtgcaa
      721 ttttgagctt cggtgtttcg gtaatttttt tgaaaagttc gggaaaaatt gaggaaaatt
      781 caatgtcttc ggcttcgctg ccgtaaatcc cccgcgccat aggggaagcc aaatactcag
      841 cggtagaaaa tcccagtacg ttgcgtgcag catcgaaaag ggtgatagtg ggccaaattg
      901 aaaatttttt acccatcgcg agaaacattt ttattttttc gggaaagaat aataaagaag
      961 aaaaaaataa cgacccggga gaagtaggcg ctttgactaa atagccgtct ttgacgacat
     1021 accgttttgc cgtgttttta gtttcatgga gtaattcgct ttcaatacct aaatcgcgta
     1081 tcatagtggt gaataatccc gaacgctcta aatacgaatc ggggccaggt tctaaaagcg
     1141 caccatcgtg ggtttgggtt tggatgttgc caccgagttt acctgcttca aaaagtatga
     1201 tttcgtcttt ggggtgcttt ttcttccagt aatatgcatg cgctaaccca gaaattccac
     1261 cgccgaccac tgctatacga gacatgattt tgtgtcataa aatttgaaca gggcgacaat
     1321 gaaaaactaa aaatggcttt acaaaaacta caactattcc agtttcaatt atgctcgtag
     1381 caatggttca agaagtcgcc acaacggcga tggggtatgc agtcatcata aaacttgaat
     1441 tcaaaaataa aataataccg gtgtttgtgg gccccaccga agcgtttgct atttctgcaa
     1501 ttttgcataa agaaacccgt gaccgaccga ttgcggttga tttacttgca acgttaatta
     1561 ccgagtcgaa ggcgcgtctg gaaaaagtgt ttatccatga ttttcaagga ggcacttttc
     1621 ttgcgcgtat ttatctaacc ggcgaacaat tcaacaaggg tgcgcttgag ctcgatgcgc
     1681 gcccttcgga tgcgcttgcg ttggcgttaa gattgcgtag ccccgtctac gtagccgatc
     1741 acgtttacga ccgcacggcg atcaaatttt ctacggtgcg tgaagcggac aacaaagcaa
     1801 tccacaccga tagcgaaagc atcgacgata tactcagcga cgacgagcgc gaagagtttt
     1861 ttgaggcgat tctcgaagaa tttggcgaac agaaaaaagc ggttaaaaaa acaaaacctg
     1921 cggttgaaaa aataaaattt ctttcgcgct ccgaagcgct tgagcgaatg ctcaaaaaag
     1981 cgcttgccga cgaacaatac gaagaagcgg cgcgtatccg tgacgagtta cgcgccgaat
     2041 cgtcaaagac aaattaaact aaagttaaaa tgagttaaga cttaatatga cttttgtcgg
     2101 tgaatttgtc gaaaaagatt tcgtgttttg acacaccaca ctcttcgagt ttttggatgg
     2161 ccgcatcaat catcgcaggc ggaccacaaa ggaaggcggc ggtgtttgca aagtcaattt
     2221 tttgttgtgc gattaactct gtcacaaagc cacgcgctcc tttccacgac gatccttctt
     2281 cttcatgcga aagaatgggc gtgaacgtga gtttaagctg cgctgcgagt tttttcatgt
     2341 cgtcgagcgc gtagagatct ttttgggtgc gcgcgccaaa gagataaata atttgcttcg
     2401 tgagcttttt ttgtgccgca tcctcaagca ttgctttaat aggagccata ccgctgccac
     2461 ccgcaatgca gagcagcgtg tctttgtggt cgcgccatcc aaacgtaccg cgcgggccgc
     2521 gtatttttat cgcttcgccg gtgcgatcct tcgagtggaa ccactcggta acttgccccc
     2581 ccggcaccaa gcggataaaa aacacaactt caaaactggc gccacccgcg cccggggcgg
     2641 cggcgaaaga ataagaacgg gtgcctttaa tgccgggtat agcgaaatct gcgtattgtc
     2701 ctgcgctaaa acgcattttt tcggcgagtt taattttgac ctcgataatg tcgtgcgtga
     2761 gtttgtggta agtactcacc gtgccagcgt actctttcga ttcatcgggt tcaagggtga
     2821 cgattaaatc agttttaggt aaagtttggc aagcgaggat gtatcctttt ttcaaatcgt
     2881 cttcggtgag gacgagcgac atatccgaca aatctttgta ttccccttgc acgagttttg
     2941 ttttacagct ggtgcaactg cctgcgccgc agctaaaggg ataatcaagc cccgcttcga
     3001 gcgcagcctc aagtaacgtt tggcggtgtt tcaccgtaac ggttttattc atgctttgga
     3061 tgtacgcact aaacgttggt ttttcttttt tcttaaaaag tgaaaacatt tcttacataa
     3121 acttattccc gaaaccgctg gacaatctct tttagattgt gcgtcaaccg tcaggtttac
     3181 agttgtcgga aagactcgta aaaaaatgct cgaaactaag tttggataaa cacactatgc
     3241 tttcacccac tttgaacgac aaccctcttt tacaccaagg cgcattgccc gactttgaaa
     3301 aaatccgaag cgagcatgtt gtaccggcgg tgcgcgctgc attaacgttg gctgaggaag
     3361 ctctggccgc cgtcgagaaa gaagaaaatc ccacttggga aaactttgtt ttaccgttgc
     3421 gtcaagcaac gcgccgcctc gaccaggcgt ggtcggcggt gaatcatctc atgggcgtga
     3481 aaaatagcga cgacttgcgt aaagcgcacg acgctgtgca aaacgaagtc gtcgcgttca
     3541 gccttaaaat gggacaaagt cgtgcgctct acaacgcgtg ggaaaaactg caaaaaaata
     3601 agagcgaact ttcaacaccg gcgcgggtgc gtattgtcga agcggcgttg cgcgatgcac
     3661 gcctttcggg gattgcgctt gaaggcaaag cgaaagaacg cttcaacgcg atccagcag
//
LOCUS       DAOMPD010000262         3704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10257_length_3704_cov_2.468073, whole genome shotgun sequence.
ACCESSION   DAOMPD010000262 DAOMPD010000000
VERSION     DAOMPD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10257_length_3704_cov_2.468073"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="PLY93_09370"
     CDS             complement(<1..1700)
                     /locus_tag="PLY93_09370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRP69726.1"
                     /translation="MAVSSEVSAFLEDLFENLSAIEEGIAQLEDEPTNQEHLNAIFRA
                     AHTIKGNASMMNLTNLVALGHALETTLQECLNARLQVDRDALRLFSECRVTMTEIGAE
                     LRSGKDGKSIAILETTDRLQALLLDEEGAAKAVSMDGVRRMQITLRIAKAELAPAVRA
                     FLAETKIAEMGTIISKEPPEEKLESPEFLASNRELHFTVDTTSSPKDIRENLNIDLIE
                     DVEIVDKGAGSAPKTVQSTARERNEMVADVQSTTSDTIRLSVQTIDRLLNLAGELVLA
                     NGGLLQLADELTTTQGAENQALRLTEKNREIFHIASEIQNIVMKSRMLPIEHVFSRFT
                     RFVRDYAESAGKMIRLETSGEKTELDKRVIDEIVKPLTHLIRNSLDHGIETSEERIAA
                     GKPPEGALKISAAQSGSSILITVEDDGRGLNAEKILKKAIEKNLIKAEEAQNLTPEEI
                     REFIFMRGFSTKEAADDLSGRGFGMDIVRESIERLSGDLSIHSVDGKGTRMTINLPLT
                     LAILSTLTFKVRNDVFALPLTVIDESLRVHDGSIVEIEGRAVLHAQDEMLPFLRLDRV
                     "
     gene            1779..2753
                     /locus_tag="PLY93_09375"
     CDS             1779..2753
                     /locus_tag="PLY93_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69727.1"
                     /translation="MAVQDTQTLRNPKVAIRSFSFSFALGILLLVEVILLGGLSVFAF
                     FSMKESARQLETAYSERGRELTLALSSNTISTDVRSLSRLSATFAAVVNKYRYNDTER
                     PISEIFILSKDGRILAHSDVSLVTTGTREAINEISAKYNQEFFHGSLSYEANSVQQED
                     LPKPSGIFQSKNSFLVDTFMSPHVYYSTDFSTALKEVDRRTRKEKSYATLHVITNRLG
                     VYYYLSAMLQKFLLVFAITFLSGFLVTGIILFAFFLRARSIQSEWKRALTYLWENEVI
                     KTEVAHGFQALSGKLNDIEKRVNQPPPALPQARVQTDILDAILIEPVK"
     gene            2755..3618
                     /locus_tag="PLY93_09380"
     CDS             2755..3618
                     /locus_tag="PLY93_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP69728.1"
                     /translation="MLEFNTALTEDILVIELEGALDSNSAADFRTWFNEKIRVGFRAF
                     ALDCLCLEYISSAGISALIDLQNQLKVQNGKLVLYQLSSETRQLLRFLQIDAKLNLCS
                     DYDAALQMLTGIKRVEKENLSTPPATENGGVAMVGVEELRVLEQTESIASTTPVQPAE
                     TPPIHEHNPNEVVVEEQLTQEKRGLHPIQETSAAVDVQTSTTENKIDIGVNPTHATTL
                     AEKEGAPPTAPVDWEETPVREAQELKIDAAAKRLITCPNCRNVLRLSVPGDYLCPSCR
                     FRFTYKGSSAT"
BASE COUNT          902 a          892 c          894 g         1016 t
ORIGIN      
        1 aacactcggt cgagccgcaa gaagggcagc atttcatctt gcgcgtgcaa aactgcgcgc
       61 ccttcgattt caacgatgct gccgtcgtga acgcgcaagc tctcgtcgat gacggtgaga
      121 ggtaatgcaa acacatcgtt gcgtactttg aaggtgagcg tcgaaagtat cgcgagtgtg
      181 aggggaagat taatcgtcat gcgcgtcccc tttccatcca ccgaatggat cgacaaatcg
      241 ccagacaaac gctcaatcga ttcgcgcaca atatccatgc caaagccacg ccccgacaga
      301 tcgtccgccg cttctttggt ggaaaaaccg cgcatgaaaa taaattcgcg gatttcttcg
      361 ggggttaaat tttgcgcttc ttctgctttt atgagatttt tttcaatcgc tttcttgaga
      421 attttttcag cattgagacc gcgtccgtca tcttcgacgg taattaaaat actgctgcct
      481 gattgcgctg cgctaatttt tagcgcgcct tcgggtggtt tacccgcagc aatcctttct
      541 tcgctggttt caatgccgtg gtcgagcgag ttgcggatta agtgcgtcaa aggtttcacg
      601 atttcgtcga tgacgcgttt gtcgagttct gttttttcgc cgctcgtttc gagacgaatc
      661 attttgcctg cgctttcggc gtagtcgcgt acaaaacgag tgaagcgcga aaagacgtgt
      721 tcaatcggca gcatacgcga tttcatgacg atattttgga tttcagaagc aatatggaaa
      781 atttcacgat ttttttcagt gaggcgcaat gcttggtttt ccgcgccttg tgttgtcgtc
      841 aattcgtcgg cgagttggag aagtccaccg ttagcaagca ccagttcgcc agcgaggttg
      901 agtaggcggt cgatggtttg cacggaaaga cgaattgtgt cgctcgtcgt cgactgaacg
      961 tccgccacca tttcattgcg ctcgcgcgcg gtggattgaa ccgttttggg cgcactcccc
     1021 gcgcctttgt cgacaatctc aacatcttcg atgaggtcga tgttcaaatt ttcacgaatg
     1081 tcttttgggg aagatgttgt atcgacggta aagtggagtt ctcggtttga cgctaagaat
     1141 tcggggcttt ctaatttttc ctcagggggt tcttttgaga taattgtgcc catttcggcg
     1201 attttagttt cggccaaaaa tgcacgtacc gctggagcca actctgcttt tgcaatgcgc
     1261 agtgtaattt gcatgcgccg cacgccgtcc atcgaaacgg cttttgccgc gccttcttcg
     1321 tcaaggagca aggcttgcaa gcggtcggtg gtttctaaaa tagcgatgga tttaccgtct
     1381 ttaccggagc gcagttctgc gccgatttca gtcatcgtga cgcggcattc tgaaaaaagg
     1441 cgcagtgcgt cgcggtcgac ttgcaaccgt gcgttgaggc attcttgtaa cgtcgtttca
     1501 agtgcatgcc ccaaagcgac caaattggtt aggttcatca tcgacgcgtt acctttaatt
     1561 gtgtgcgccg cacgaaaaat ggcgtttaag tgttcttggt ttgttggctc gtcttcaagc
     1621 tgagcgattc cttcttcgat tgctgaaaga ttctcaaata aatcttcgag aaatgcagaa
     1681 acttcagaac taacggccat agcttatttt cggctttgaa aagcgtataa tagagaaatt
     1741 attatctaaa cgttttcctt tttttccgat agataagtat ggcggttcag gatactcaga
     1801 cattacgtaa cccaaaggtg gcaatccgta gcttttcatt cagttttgca ttgggtattt
     1861 tgctgctcgt cgaagtaatt ttactcggtg gcctttcggt ttttgctttc ttttcgatga
     1921 aagaaagcgc gcgccagctc gagacggctt attccgaacg ggggcgtgag cttacacttg
     1981 ccttatcatc caacactatc tcaaccgatg ttcgatcgct ttctcgcttg tccgctactt
     2041 ttgcggcggt tgtcaataaa taccgctaca acgacaccga acgccccatt tctgaaattt
     2101 ttattttatc taaggacgga cgtatcctcg cacactccga cgtgtcgctg gtgacaacag
     2161 gcacgcgcga ggcaatcaac gaaatatctg caaaatataa ccaagaattt tttcacggtt
     2221 cgctatctta tgaggccaat agcgtgcagc aagaagattt acccaaacct tcgggaattt
     2281 ttcaaagtaa aaattcgttc cttgtcgaca cgttcatgag cccgcacgtt tattactcca
     2341 ccgatttttc caccgcgctt aaagaagtgg atagacgcac tcgaaaagaa aaatcgtacg
     2401 caacgctgca cgttattaca aaccgcttgg gtgtgtacta ttatctttca gccatgttac
     2461 aaaagttttt acttgttttt gcgattactt ttttgagcgg ctttcttgta accggtatta
     2521 ttctctttgc tttctttttg agggcgcgtt cgattcaaag cgaatggaag cgtgcgctca
     2581 catacctttg ggaaaacgaa gtgattaaaa cggaagtggc gcatggcttc caagcgcttt
     2641 ccgggaaatt gaacgacatt gaaaagcgcg tcaaccaacc gccgcctgca ttgccacaag
     2701 cgcgcgtgca gactgatatt ttagacgcga ttttaatcga acctgtgaaa taaaatgtta
     2761 gagtttaaca ccgcgctcac tgaagatatt ctggttattg aattagaggg ggcgctcgat
     2821 tccaatagcg ccgctgattt tcgtacgtgg ttcaatgaaa aaatacgcgt ggggtttcgt
     2881 gcttttgcgt tagattgcct ctgcttagaa tatatttcct ctgcgggtat ttctgcactc
     2941 atcgacctgc aaaatcaact gaaggtacaa aatggtaaac ttgtgctgta ccaactttcg
     3001 agcgagacac gccagctctt gcgttttttg cagattgacg caaaattaaa tttatgtagc
     3061 gactacgacg ccgcgctaca gatgctcact ggcattaaaa gagtagaaaa agaaaatcta
     3121 tcgacgcccc ctgcaaccga aaacggtggc gtggctatgg tcggtgtgga agagttgcgc
     3181 gtcctcgaac aaacagagtc tatcgcatcc accacgcctg ttcagcctgc cgaaacgccc
     3241 cccatccacg agcacaaccc caatgaagtg gtcgttgaag aacagctcac tcaggaaaag
     3301 cggggattac accccatcca agaaacaagc gcggcagtgg atgtgcaaac aagcaccact
     3361 gaaaataaaa tcgacatcgg tgtgaatccc actcatgcca caactcttgc tgaaaaagaa
     3421 ggcgcgccac caacggcacc ggtggactgg gaagaaaccc ccgtgcgcga ggcgcaagag
     3481 ttaaaaatcg acgcggcggc aaagcgactc atcacttgcc ccaactgccg caacgttttg
     3541 cgactttcgg tgccgggcga ttacttgtgc ccgtcgtgtc ggtttcggtt tacgtataag
     3601 ggaagttcgg ctacataagt gaccaagctg taactcgttc cgtcaatttt gtctaaccca
     3661 cggacatacc tgacaaatgc tacgccgcaa ttggggggta tttt
//
LOCUS       DAOMPD010000263         3677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10361_length_3677_cov_2.738818, whole genome shotgun sequence.
ACCESSION   DAOMPD010000263 DAOMPD010000000
VERSION     DAOMPD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10361_length_3677_cov_2.738818"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLY93_09385"
     CDS             complement(<1..391)
                     /locus_tag="PLY93_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP69729.1"
                     /translation="MSTIAHSIGHAADAHDEHYLSKKGIMSWLSSVDHKRIGLLYLFT
                     GLAAFFVGGIFALLLRTELLFPGHTFIDEKTYNVFFTLHGSIMIFIFIVPGIVATFGN
                     IFLPIMIGAREVAFPVINRLTYQLYLIG"
     gene            complement(400..1482)
                     /locus_tag="PLY93_09390"
     CDS             complement(400..1482)
                     /locus_tag="PLY93_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRP69730.1"
                     /translation="MGDFFANVFGFISHAPPASTLAPDVKFAADFINGISLVAFILVE
                     GVLIYFIIRYRRRKGEPDKQTPYITHHTTAEIIWTVLPTIILGIIFYFGISSFLKLRS
                     MPKDGEIIELTARQWAWDIKYPYCVKNPKTNSEKCIKLSSVNVNPTADHPNRLQDEAM
                     KDQVRFLIAKGRKYVMQMTSTDVIHSFTVPSFYVKQDVVPGLTSRMWFEPTKVGEFVI
                     TCNEYCGDKHSGMLGIFKVVEPAEFDAWKNAQLAAITKAMSSEGEKVDESVLIAKGQE
                     LYKGKGCVACHSIDGKRGAGPSFKGIYGKTETLTTGSVLVDDAYLKESMLKPSAKVVK
                     GFAAMPPMPIEEDETKYLIAFIKSLK"
     gene            complement(1553..2404)
                     /locus_tag="PLY93_09395"
     CDS             complement(1553..2404)
                     /locus_tag="PLY93_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP69731.1"
                     /translation="MRWLSLFIPLFCIVTLYADLPPADDTLAKTPPPLEVTVEEKLGA
                     PLASMQFFDEEGKPIKLSDLADGHRPIVIAPVYYNCPHLCTLTLNGLMSAIEKETRYK
                     LGKDYLVVAYSFHPDEKFQLAQVKQANYLRKLSEVTDQNLPEYRKKWRFFTGNVEGIA
                     AMSKAIGFKYLKEVSTNLKKKDEEYIHPAVLVVTTPDLKISRYLYGVEYSPIDFKMSL
                     LEAADGKITKSVSDRLLLTCYSFDPIKRKYSLVAWRVMRLGGIVALVLLGALLAVLWV
                     REKRRRA"
     gene            2509..2958
                     /gene="dut"
                     /locus_tag="PLY93_09400"
     CDS             2509..2958
                     /gene="dut"
                     /locus_tag="PLY93_09400"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRP69732.1"
                     /translation="MSIVRIQFIRLSTNALLPARQSTNAAGYDIYAALDAPHTLARGA
                     IFPVPTGIALEISAGFHVSIRARSGLAVKHGITPVNSPGTIDADYRGEIFVPLINLGS
                     EAFTIHHGDRIAQLLLEKTYDIEWQEVDTLTNTTRGDGKFGSTGLAR"
     gene            2924..>3677
                     /locus_tag="PLY93_09405"
     CDS             2924..>3677
                     /locus_tag="PLY93_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP69733.1"
                     /translation="MANLVPQVSPVNAGILDFPARLRPNKLYFAFGGGVGRFGTNFTL
                     FHLNDTTVWVDLGSGFANHHTPGMDKNLPNRQLLLGFPPTAIFLTHGHEDHIGALPHL
                     TEVIPHGTPIYTSPFTKALVVNRLKDRGIDPSIWEFKLIEENTQVKIGNFTVFTFFMP
                     HSIPQCFSVGLEADAAGEKKRIYFSSDFKLKGGEVRHKVAQIKKFAPVDFLFIDSTGA
                     LNEGETVDEKEVDESLERLILKIKGRIFLTTFS"
BASE COUNT          944 a          864 c          851 g         1018 t
ORIGIN      
        1 taccaatgag atataattga taggttaaac ggttgatcac cggaaatgcg acttcgcgtg
       61 cgccaatcat aatcggtaga aagatgttac caaacgtcgc gacaataccc ggaacaataa
      121 agataaaaat catgatcgat ccgtgtaacg tgaaaaacac gttatatgtt ttttcgtcga
      181 tgaacgtgtg tccggggaaa agaagttcgg ttcttaaaag aagcgcaaaa ataccgccga
      241 caaaaaaagc ggcgagaccg gtaaaaagat aaaggagtcc gattcgttta tgatcgacgc
      301 tcgaaagcca agacataatt cctttcttgc ttagataatg ttcgtcatgt gcgtctgctg
      361 catggcctat actgtgtgct attgttgaca tcgttcctcc tacttgagtg atttaataaa
      421 tgcgattaag tatttcgttt cgtcttcttc gatgggcatc ggtggcatcg ctgcaaatcc
      481 tttcaccact tttgccgagg gtttcagcat cgattctttc aaataagcgt cgtcgacaag
      541 cacggaaccc gtggtcaacg tttctgtttt gccgtagatg cctttgaacg aagggcctgc
      601 accgcgtttg ccgtcgatag aatgacaggc gacgcagcct ttgcctttgt aaagttcttg
      661 tcctttggca atcaacacgc tttcgtcaac tttttcacct tcgctgctca tcgctttggt
      721 gattgcggcg agttgtgcat ttttccatgc gtcaaactcg gcgggctcga ccactttgaa
      781 aatccccaac atgcccgagt gtttatcgcc gcagtattcg ttacaagtga tgacaaactc
      841 accgaccttg gttggttcaa accacatccg cgaggttaaa ccagggacta catcttgctt
      901 tacataaaaa gaaggaacag tgaagctgtg tatgacatcg gtggatgtca tttgcatcac
      961 gtatttccgg ccttttgcaa tgagaaagcg cacttgatct ttcatcgctt cgtcttggag
     1021 acgattgggg tggtcggcag tgggattgac gttaacagaa gacagcttta tacatttttc
     1081 agagtttgtc ttagggtttt ttacgcaata aggatatttg atatcccatg cccactggcg
     1141 tgctgtcaat tcaatgattt caccgtcttt tggcattgac cgtagcttta agaaagagga
     1201 gatgccaaaa tagaaaataa tgcccaagat aatcgttggc agaacagtcc atataatctc
     1261 agcggtggtg tggtgcgtta tgtacggcgt ttgtttgtcg ggttcgccct tgcgccgacg
     1321 gtaacggata ataaaataaa tgagcacacc ttcaactaaa atgaacgcga ctaagctaat
     1381 accgttgatg aagtcagcgg caaacttgac gtcaggcgct aaagttgatg cgggcggtgc
     1441 gtggctaata aacccaaaca cattcgcaaa gaaatctccc atagataacc cctcaatttg
     1501 ttagcgtcag tgtttggaga cagcgaattg gccatcccaa cactcacacg aattatgcgc
     1561 gtcggcgttt ttctctcacc cacaacacgg ctaagagggc tcccagcaaa acaagcgcga
     1621 caatgccccc taagcgcatc acgcgccagg cgaccagact atattttcgc tttatggggt
     1681 caaaactata acaagtcaat agtaaacggt cgctcactga ttttgtgatt tttccatcgg
     1741 ctgcttctaa aagagacatc ttaaaatcaa tcgggctata ctcgacaccg tataaatacc
     1801 gcgatatttt taagtcgggg gtagttacca ctaacacagc gggatggata tattcttcgt
     1861 cttttttctt gagatttgtt gaaacttctt ttagatactt aaatccaatc gctttcgaca
     1921 ttgccgcaat gccttcgacg ttgccggtaa aaaaacgcca ttttttgcgg tactcgggta
     1981 aattttgatc ggtgacttcg gagagtttac gcaagtagtt tgcttgcttc acttgtgcga
     2041 gttgaaattt ttcatcgggg tgaaaactgt acgcgacaac gaggtaatct tttcctaact
     2101 tgtaacgcgt ttccttttca attgcactca ttaaaccgtt gagcgtcaac gtgcagagat
     2161 gggggcagtt atagtaaaca ggggcgatga cgatggggcg gtgtccgtcc gccaagtcgc
     2221 tcaatttaat cggtttgcct tcctcgtcaa agaattgcat cgacgccagc ggtgcgccga
     2281 gtttctcttc cacggtgact tccagaggcg gtggagtttt tgcgagcgtg tcgtcggcgg
     2341 gcggcaaatc ggcgtaaagg gtcacgatac aaaaaagtgg gatgaataac gataaccagc
     2401 gcatgccacc ggtttgaatt tatgcgtcag tgtgtattgt gttaattgaa atttgtggtg
     2461 gttgtttgag tttacgcgct gtgtcttctt taattaatta taatatcaat gtctatcgtt
     2521 cgtattcaat tcattcgatt atccaccaac gctctcttgc ccgcacggca aagtacaaac
     2581 gcagccggct acgatattta cgccgcgtta gatgcgccgc atactcttgc acgcggcgcc
     2641 atctttcctg tgcccaccgg cattgctctc gaaatttcag cgggcttcca cgtctccatc
     2701 cgtgcacgtt cggggcttgc ggtgaaacac ggcatcactc ctgtcaatag tcctggcacc
     2761 atcgacgccg attatcgtgg tgaaattttt gtgcccctca tcaatttggg gagcgaggcc
     2821 ttcaccatcc accacggtga tcggattgcg caactccttc tcgaaaaaac atacgatatt
     2881 gaatggcaag aggtcgatac tttgactaat acaacaagag gagatggcaa atttggttcc
     2941 acaggtctcg cccgttaacg caggcattct cgatttccct gcacgtttac gccccaacaa
     3001 actttatttt gcgttcggcg gcggcgtggg acgcttcggc actaacttca ccctctttca
     3061 tttgaacgac accacggtgt gggttgactt gggttcgggg tttgccaacc accacacgcc
     3121 ggggatggac aaaaatttac ccaaccgcca actcttgtta ggatttccgc cgactgccat
     3181 ttttttaacg catggccacg aagatcacat cggcgcgttg ccgcatttga ccgaggttat
     3241 tccgcacggc actcccattt atacgtcgcc ttttacaaaa gcgctcgtcg tcaatcgtct
     3301 caaagataga gggatcgatc cttcaatttg ggaatttaaa ctcattgaag aaaacaccca
     3361 agttaaaatt gggaatttta ctgtatttac ttttttcatg ccgcactcga taccgcaatg
     3421 cttttctgtg gggcttgaag ccgatgctgc cggcgagaaa aaacgcattt atttttcgag
     3481 cgacttcaag ctcaaaggcg gtgaagtccg ccataaagta gcgcagatta aaaaatttgc
     3541 gcccgtcgat tttcttttca tcgactcgac gggcgcgctc aatgaaggcg aaacagtcga
     3601 cgaaaaagaa gtcgacgaat cgctcgaaag actcattttg aaaatcaaag gccgtatttt
     3661 tttgacgaca ttctcat
//
LOCUS       DAOMPD010000264         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10438_length_3652_cov_3.033361, whole genome shotgun sequence.
ACCESSION   DAOMPD010000264 DAOMPD010000000
VERSION     DAOMPD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_3652_cov_3.033361"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /gene="cysS"
                     /locus_tag="PLY93_09410"
     CDS             complement(<1..948)
                     /gene="cysS"
                     /locus_tag="PLY93_09410"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRP69734.1"
                     /translation="MVLNLYNTLNKKKESIEGQSVKIYSCGPTVYNYAHIGNFRSYVF
                     ADVLNRTLKLAGYRVEHAMNLTDVDDKTIKGAAELVQKEPSKYVNMKAALAAYTAPYT
                     EAFFADLQTLNIVKFDHHPRATDHIPAMLDLVERLVKAGVAYEQDGSVYYRIADKKDY
                     GKLSKIDLEKNRTGTRYNTDEYDKDDVRDFVLWKAETPSGVHWHSHHGDGRPGWHLEC
                     SAMIHEIFKGEIDIHTGGVDLIFPHHENEIAQSEAAYGNGFVKHWAHCEHLLVDNRKM
                     SKSAGNFYTLRDLLTQGKRPEALRYFLLSAHYRQKINFTE"
     gene            complement(992..1063)
                     /locus_tag="PLY93_09415"
     tRNA            complement(992..1063)
                     /locus_tag="PLY93_09415"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Pro,seq:ggg)
     gene            1225..1869
                     /locus_tag="PLY93_09420"
     CDS             1225..1869
                     /locus_tag="PLY93_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69735.1"
                     /translation="MVTMEQLEQLEGRIVKALELISDLRVENSHLESESERLKASNDQ
                     LRLTAEEREREAVKWQQQLQEATQELSRLKSKEQELETRILAIIAKLDNLKAPEKPAA
                     ASSTNTEPTKAEPASAATESAKEPSSANNTSKDAVNDKDEIILDEEDDEIILLDDEEE
                     IIADKKPATTEEKKETTTAPSDEIIIEDDDVLGVFDDDDDEDFLIVEEEMSEKK"
     gene            1938..2435
                     /locus_tag="PLY93_09425"
     CDS             1938..2435
                     /locus_tag="PLY93_09425"
                     /inference="COORDINATES: protein motif:HMM:NF027443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HRP69736.1"
                     /translation="MALGFLPIVGFNFTLYAEMQSKSVFYIQRNKNRNEVHYALRLNP
                     EACVPVGKHPLFAYWRNLEISPTAIEPIGRFESIAYGIASQELKGNDLFVKLRAFPER
                     IIRIHFEKNPRCTATAHVTINGRESELKFIYVFAKEGLIKPTVIYVDINGQLNGVKVS
                     ERILR"
     gene            2449..3072
                     /locus_tag="PLY93_09430"
     CDS             2449..3072
                     /locus_tag="PLY93_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRP69737.1"
                     /translation="MLGAILGFASGFLGSVPVTGPIALLVFRSSLRGSFSSAMHIVLG
                     ASIAEALYCALATFGYIQIITAYPFLTKYIRYMGSIFLLLLGVLFLFQKIKIVDEETP
                     VVSANEKRGGFFTGFMISLLNPTLFLTWGSATSTIFSWFNAITVLDMVLFPIAAGLGI
                     VCWFFLLLEIFKKFRVKIGERIGYFAIRGAALLLIGLGIYMLSQVNQ"
     gene            3069..>3652
                     /locus_tag="PLY93_09435"
     CDS             3069..>3652
                     /locus_tag="PLY93_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP69738.1"
                     /translation="MKCLFFLCVPFFWVCRSAPVTLNDNSIESVYEKVLAQSAVKKKL
                     LDIDIASKNFTIEELFILSLDRTEQLAIAAERTSAADAGVRKSYGAWLPRISMIAAQY
                     APLTPGFVVPGVRFSARQNIMTGLNEYTGVVGARLTRVSEEQNLRAEMAAHLLNIADA
                     ALQLKFTQTLRAQTAEIVKLSASNLAEIRRRISIG"
BASE COUNT          956 a          817 c          876 g         1003 t
ORIGIN      
        1 ttcagtaaaa ttgattttct ggcgatagtg tgccgagagc agaaaataac gcagagcctc
       61 ggggcgtttg ccttgcgtga gtaaatcgcg tagcgtgtaa aaattacccg ccgactttga
      121 cattttgcgg ttgtcgacca acaggtgttc acagtgcgcc cagtgcttca cgaagccgtt
      181 accgtatgcg gcttcgcttt gcgcgatttc gttttcgtgg tgcggaaaga ttaaatccac
      241 gccaccggta tggatgtcta tttcaccttt gaaaatttcg tggatcatcg ccgagcattc
      301 gagatgccaa cccgggcggc cgtcgccgtg gtgcgaatgc caatgcacgc cggaaggagt
      361 ttcagccttc cagagtacaa aatcgcgcac gtcgtcttta tcgtactcgt cggtgttgta
      421 acgcgttccg gttctatttt tttcgagatc aatcttcgag agcttgccgt aatctttttt
      481 gtcggcgatg cggtaataga ccgaaccatc ttgttcgtat gcgacgcccg ctttaacgag
      541 gcgttcaacc aagtcgagca ttgcggggat gtggtcggtc gcgcgcgggt ggtgatcgaa
      601 ctttacgatg ttgagcgttt gcaaatcggc gaaaaatgct tcggtgtagg gagcagtgta
      661 cgcggcgagc gcggctttca tgtttacata tttactcggc tctttttgta caagctctgc
      721 tgcgcccttg atggtcttat cgtccacgtc ggtgagattc atcgcgtgtt cgactcgata
      781 ccccgcgagc ttcaacgtgc ggttcagcac atcggcgaag acgtagctgc ggaaatttcc
      841 gatgtgcgca tagttataca ccgtcggccc gcaggaataa attttgacgg attgcccctc
      901 gatggattcc ttttttttat tgagtgtatt atataggttg agcaccataa agtggggttt
      961 ttaagggggg cgcagccccc cttttttcat ttcgggctga ctggatttga accagcgacc
     1021 acacgccccc cagacgtgtg cgctaccact gcgctacagc ccgttttatg ctttcgcata
     1081 gagtcgactc attcgtcgcc gaggcgataa taaatcaacc atacgcgtaa gcaacaataa
     1141 aagacaggcc gtcaatcgat aagaagtata ttaaaactca aaaaacgctt gtccgctgcg
     1201 cttaactcaa agtaaggtca acgtatggta actatggaac agcttgaaca actcgaaggt
     1261 cgtattgtca aggctctcga acttatttcc gacttacgcg ttgagaatag ccaccttgaa
     1321 tccgagtcag aacggctaaa ggcaagcaat gatcaattac gcctcaccgc cgaagaacgc
     1381 gaacgcgaag cggtgaagtg gcagcaacaa ttacaagagg caacgcaaga gttgagccgt
     1441 ttgaaatcta aagaacaaga actcgaaact cgaatactgg caattattgc aaaactcgac
     1501 aaccttaaag ctcctgaaaa acccgccgct gcctccagta ccaacaccga acctacgaaa
     1561 gccgagccag cgtcagcagc tacagaatcg gcaaaagagc ctagcagcgc gaataacacc
     1621 tcaaaagatg cggtaaacga taaagacgaa atcattcttg acgaagaaga cgacgaaatt
     1681 attttactcg acgacgaaga agaaattatc gccgataaaa agccggcaac caccgaagaa
     1741 aaaaaagaaa caacaaccgc tccttcggat gaaattatca tcgaagacga cgatgttttg
     1801 ggcgtctttg acgatgacga cgacgaagac ttcttaatcg tcgaagaaga aatgtcagaa
     1861 aaaaaatagt ctcatccacg aatatctcta tcattttcaa ggttatcacg caggctttca
     1921 ataaagtacc catcgcaatg gcgttgggat ttttgccgat tgttggtttc aattttactt
     1981 tgtatgcgga aatgcaatcg aaaagcgtat tttatatcca gcgcaataaa aacagaaacg
     2041 aagtccatta tgctttacga ttaaatcctg aagcttgtgt acctgtcggt aagcatcctc
     2101 tctttgccta ttggcgcaat cttgaaatta gccccaccgc gatagaaccc attggtaggt
     2161 ttgaaagtat tgcctacggt attgcatcgc aagaattaaa gggaaacgat ttatttgtga
     2221 agttgcgcgc ttttcccgag cggattatcc gcattcattt cgagaaaaac ccgcgctgta
     2281 ctgctacagc gcatgtaaca atcaacgggc gcgaaagcga actaaaattt atttacgtgt
     2341 ttgcgaaaga agggcttatt aagccaacag tgatctacgt tgacatcaac ggtcaattaa
     2401 acggagtgaa agtgtctgaa cgcattctgc ggtgaggcac aggtaatttt gttaggcgca
     2461 attttgggat ttgccagcgg ttttttaggc tcggtgcctg ttacaggtcc gatagcgctt
     2521 ttggtttttc gttcaagtct gcgtggtagt ttttcttctg caatgcatat tgttctgggc
     2581 gcgtcgattg cagaggcgct ttattgcgcg ttagcgacgt ttgggtatat tcaaattatt
     2641 acggcatatc cttttttgac aaagtatatt cgctacatgg gctcaatttt tcttttattg
     2701 ttgggagtgc tatttctttt tcaaaaaatt aaaattgtcg atgaggaaac ccccgttgtg
     2761 tcagcaaatg aaaaaagagg gggatttttc actgggttca tgatttcact tttgaaccca
     2821 acactttttt taacttgggg aagcgcgacc tctacaattt tttcatggtt taacgctata
     2881 acggtattag atatggtttt gttccccatc gcagcagggc ttggtattgt ttgttggttt
     2941 tttttattgc ttgaaatttt taagaaattt cgtgtgaaaa ttggcgaacg tatcggttac
     3001 ttcgccattc gtggcgcggc tcttttacta attggcttag gaatttatat gttatcacag
     3061 gtaaatcaat gaaatgctta ttctttcttt gcgtaccttt cttttgggtt tgccgcagcg
     3121 cacctgtaac gctaaacgat aattctatcg aatcggtgta cgagaaagtg cttgctcaat
     3181 cggcggttaa aaaaaagtta ttggatatcg atatcgcatc taaaaatttt acgattgaag
     3241 agttattcat cctctcgctc gaccgcactg aacagcttgc cattgccgcc gagcgcacca
     3301 gcgctgccga cgcaggagtg agaaaaagtt acggtgcgtg gttgccgcgt atttcgatga
     3361 ttgccgcgca atacgcaccc ttgactccag ggtttgtggt gcctggggtg cgtttttcgg
     3421 cgcgccagaa tatcatgacc ggccttaacg aatatactgg agtcgtgggc gcacggttga
     3481 cgcgtgtttc agaagagcag aatttgcgtg ctgagatggc ggcacatctc ctcaacattg
     3541 ccgacgcggc actccaactt aaatttactc aaacgttgcg cgcacaaacc gctgagattg
     3601 taaagctgag cgcttccaat ttagccgaaa tacgccgtcg tataagcatt gg
//
LOCUS       DAOMPD010000265         3649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10449_length_3649_cov_2.319699, whole genome shotgun sequence.
ACCESSION   DAOMPD010000265 DAOMPD010000000
VERSION     DAOMPD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10449_length_3649_cov_2.319699"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PLY93_09440"
     CDS             complement(<1..1188)
                     /locus_tag="PLY93_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69739.1"
                     /translation="MDSLAAAFALFRRGEFAESLAAALILCEREAQNFWARYLAAISA
                     AFEPNLKAFEKHLNELDAFETQNVYFHYLKAYYALLQNDVEKALWHYLQIVDDPEGWL
                     ARSLVKKFRKIKTIENVAFRAGDYIVLPPELPPPIEFRKKETDSDEEKISTLSVRPTI
                     IYEPVRENISREKNIAPLIKFIFLKKILEKNKRKFFIATIALTGLALSALAIYAGIRF
                     SLSRPQEIRVVDFQIADSAAVMPVVDPKNTLYTYKTREAIIGDFEKAKQQLKEKKVNQ
                     ARYLLNRLLYSNADFQTREKARTFLGFIPEPAFTEFRDNIPLAKLLKAPKLRADSLLV
                     FSGVVNDAYNDPNGKGSIYKLVVSESDQEYLVHAFSAEKIIAGNTRRQSVQIYGRFKG
                     LVGE"
     gene            complement(1192..1758)
                     /gene="efp"
                     /locus_tag="PLY93_09445"
     CDS             complement(1192..1758)
                     /gene="efp"
                     /locus_tag="PLY93_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRP69740.1"
                     /translation="MINSNHIKRGMALKVDGDLVVIQSFEHHKPGKGAAIVRVRIKSL
                     SKGTTVERTWRSGEMLEDVELEKRNATYNFEDGDQLVFMDSETYDQIHVDKKDLEDIL
                     VYISDGMEFQILLYDDKPVSVIPPNFVTVKVEYAEEGLKGDTATTAYKEVTVEGGGKI
                     QTPLFVKTGDMIKIDLRDLSYVERVNQK"
     gene            complement(1958..2767)
                     /locus_tag="PLY93_09450"
     CDS             complement(1958..2767)
                     /locus_tag="PLY93_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69741.1"
                     /translation="MAKGARGSKNNGADGQHLITILFVLGVIGWGFFAIDRLTDPEET
                     SRIGVNSASARGHSVDNSSWRKNLRAWLSKKLSVEKQTVVSKPQRVSLNIPLEQEPQN
                     LTREDLEETLTQVDIKEEPLAEKPSQNEVSVLFYRLDKKGQPTLSMVKRAIDAEKRSL
                     RSMLTLLIKGPTLNEQDRDYIDSFIRKPRVLSAGVHRECVYIDFDKNFGSGVSYQTMR
                     FQIQQLFTNATVWTKTSCLELRVQGKYTPHLGSDGLFFPKRIDAVWLKENL"
     gene            complement(2856..3347)
                     /locus_tag="PLY93_09455"
     CDS             complement(2856..3347)
                     /locus_tag="PLY93_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69742.1"
                     /translation="MKIKILIKAQSEAALKQTNKTLFVFLLLLFTNVCTPLRDSVLKD
                     VERNYSTVGFLDVDTYQIFCPLLSEGDRVQKCKEALLNGLVAYKERYEHEAHQRRTHT
                     DFTPFITTEKISNADRERWRTSFSVFMKNHTRLVYEEYKNNSFSGVFRLRIKDLIYRV
                     QEV"
     gene            complement(3373..>3649)
                     /locus_tag="PLY93_09460"
     CDS             complement(3373..>3649)
                     /locus_tag="PLY93_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRP69743.1"
                     /translation="EVIFEEFKGTGNMELHLDRKLVEKRTFPAIDMNRSGTRREELLL
                     DKDDVNRIWILRKIFASLSTVESMELLLEKMRGTKNNKAFLDSMKTG"
BASE COUNT          982 a          851 c          760 g         1056 t
ORIGIN      
        1 ttcgcccacc agtcctttga accgcccata aatttgtacc gactgtctgc gcgtatttcc
       61 tgctattatt ttttcagcgc taaaagcatg aactaaatac tcttggtcgc tttcgcttac
      121 cacaagcttg taaatactcc ctttcccatt cgggtcgttg taagcatcat taactacccc
      181 agaaaaaacg agtaaactgt cggcgcgtaa tttaggcgct ttcaaaagtt ttgccaacgg
      241 aatgttgtca cggaactctg taaaggctgg ctcggggata aaacccaaaa aagtacgagc
      301 tttctctcgg gtttgaaaat cggcattcga gtacaagagg cggttgagta aataacgcgc
      361 ttggtttact tttttttctt tgagctgttg ttttgccttt tcaaagtcac cgataatcgc
      421 ctctcgggtt ttatacgtat agagcgtatt tttcggatca acaacgggca tcactgcggc
      481 agaatcggcg atttgaaaat cgacgacacg tatttcttga gggcggctta aagagaaacg
      541 gatgcctgca taaatagcca gcgcagaaag agctaaacct gtaagcgcaa tggtagcgat
      601 gaaaaacttt ctcttatttt tctctaagat ttttttcaaa aaaataaatt ttatcagtgg
      661 tgcgatattt ttttccctgg aaatattctc gcgcacaggt tcgtaaataa tcgtcggcct
      721 tacggaaaga gttgaaattt tttcttcgtc gctatccgtt tcttttttcc taaactcgat
      781 gggtggcggt aactcaggcg gcaacacgat atagtcgcca gcacgaaatg cgacattctc
      841 gatagtttta attttacgga attttttaac gagcgaacgc gcaagccagc cttcgggatc
      901 gtccacaatt tgcaaatagt gccacaacgc cttctccacg tcattttgca gcaacgcgta
      961 atacgctttc aaataatgga aataaacatt ctgtgtttca aaagcgtcta actcgttgag
     1021 gtgcttttca aaagctttga gattgggctc aaacgccgcg ctaattgcgg caaggtagcg
     1081 cgcccaaaaa ttttgtgcct cgcgctcgca gaggataagc gcggcggcaa gcgactcggc
     1141 aaactctcca cggcggaaca atgcaaaagc cgccgccaga gaatccatcg attacttttg
     1201 gtttacgcgc tccacataac tgagatcgcg taaatcaatt ttaatcatgt cgccagtttt
     1261 tacaaacaac ggcgtttgaa ttttaccgcc gccttcgacc gtcacttctt tgtaagcggt
     1321 cgtagcggtg tcgcctttta atccttcctc agcatactcc actttgaccg tcacgaaatt
     1381 gggaggtatc acgctcactg gcttatcgtc gtagagcaaa atttggaatt ccataccatc
     1441 ggagatgtaa actaaaatat cttcgagatc ctttttatcg acgtgaattt ggtcgtaggt
     1501 ttcgctatcc ataaaaacga gttgatcgcc gtcttcaaag ttatacgttg cgttacgttt
     1561 ttccagttca acgtcttcga gcatttcgcc cgaacgccaa gtgcgttcaa ccgttgtgcc
     1621 tttgctgagt gacttgatgc gtacgcgtac aatcgccgcc cctttacctg gtttgtggtg
     1681 ttcaaaactt tgaatgacga cgaggtcgcc gtccacttta agcgccatac cgcgcttgat
     1741 atgatttgag ttaatcatgc ggatggatag acgttggcga tatagcgaaa agccgtaatt
     1801 aaaaataaaa acccgcttac cccgctggat ttttttctgt cagggtaagc gggcgagaag
     1861 acgcccaata attcacttat cgggaagacc tccactggga ggaggttcca accagtatat
     1921 cgacaaagac ggaacagaac ttaactttct tatggaatta taaattctct ttgagccata
     1981 cagcgtcgat gcgcttggga aaaaaaagtc cgtcactccc taaatggggc gtgtatttcc
     2041 cttgcacccg caactcaaga cacgatgttt ttgtccacac agtggcgttt gtgaataatt
     2101 gctgaatttg aaatcgcatg gtttggtagc tcacccccga accaaaattt ttatcaaaat
     2161 cgatatatac acattcgcgg tgtaccccgg cgctcaagac tctgggtttt cgaataaaag
     2221 aatcgatata gtcgcggtct tgctcgttaa gcgttggacc tttaataagt agagtgagca
     2281 tcgaacgtaa actgcgtttt tcggcatcga tagcacgttt gaccatcgac aatgtaggtt
     2341 gccccttttt gtctaatcga taaaataaaa ccgacacttc gttttgcgat ggcttctcgg
     2401 ccaacggctc ttccttaata tcaacctggg tcaatgtttc ttccaaatct tctcgcgtta
     2461 aattctgtgg ctcttgctca aggggaatgt tcaacgaaac gcgctggggt ttacttacaa
     2521 cagtttgctt ctctactgaa agtttttttg agagccatgc gcgtaaattt ttacgccaag
     2581 atgaattatc gacactatgc cctcgtgcag aagcgctatt gacgccgata cggcttgttt
     2641 cttcggggtc ggtgagacga tcgatagcaa aaaatcccca accaataact cccaaaacga
     2701 aaagaatcgt gatgaggtgt tgcccatccg caccgttatt ttttgagcca cgcgctcctt
     2761 ttgccacagt ttactatcga cattttaggg gggtagactt gaaagcactt atgagccttt
     2821 gcgctaactc gctgcgcgag ttggtacaaa ggctcctata cttcttgaac gcgataaatt
     2881 aaatctttta tccgcaggcg aaagactccc gaaaatgaat tatttttata ttcctcgtaa
     2941 acaaggcggg tgtgattttt cataaaaaca ctaaacgaag tacgccaacg ctctctatcc
     3001 gcatttgata ttttctcagt ggttataaaa ggagtaaaat ctgtgtgcgt acggcgttgg
     3061 tgcgcttcgt gttcataacg ttctttgtaa gcaactaaac cattcaacag cgcctctttg
     3121 catttttgaa cgcggtcacc ttcactcaaa agtgggcaaa aaatctggta cgtgtccaca
     3181 tcaagaaaac caacggttga ataattgcgc tccacatctt tcagcacgct atcgcgcaaa
     3241 ggggtacaca catttgtgaa aagcaaaagg agaaagacaa acaacgtttt gtttgtttgc
     3301 ttcaatgcgg cttcagactg cgcttttatt aaaattttta ttttcataaa agcgacaaaa
     3361 acctaccgat atctaccccg ttttcatgct atctaaaaat gccttattat tttttgtacc
     3421 acgcattttt tccagcaaga gttccatact ttcgacagtc gaaagcgaag caaaaatttt
     3481 gcgcaaaatc caaatgcgat tcacatcgtc cttatcgagc aacaactctt cgcgtcgtgt
     3541 acccgagcgg ttcatatcga tcgctggaaa tgtccgcttt tccaccaact tacggtcaag
     3601 atggagctcc atattacccg tacccttaaa ttcttcaaaa atcacctcg
//
LOCUS       DAOMPD010000266         3649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10450_length_3649_cov_2.271564, whole genome shotgun sequence.
ACCESSION   DAOMPD010000266 DAOMPD010000000
VERSION     DAOMPD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10450_length_3649_cov_2.271564"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1044
                     /locus_tag="PLY93_09465"
     CDS             82..1044
                     /locus_tag="PLY93_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP69744.1"
                     /translation="MLHAQTALPVFSLGRMTLGSESIFEDLKSPRVGLDRKNRYRVAG
                     YAQFDSKIFDTLLLSPGIRYDKDSQFGDYVSPRFGAKWGTEDFILRASAGMGYRSPSF
                     RELYLYFDNPSAGYTVHGNASLKPENSQSVNVGVEWYATRRISVDSNAYYNNLTNLIQ
                     TATAGTIGTITQYAYKNVAHAFTTGSDNRITVYLTRFLRATLGYSYTFAWDNSIQREL
                     EGRARHRGNLGLLLRYHRLTLHAALALVDRRPYYTDEEGKPPTQTPIYSRAYATVDVN
                     GEYAFLNGLSIFAGGENLNSAGDAAYLPLPPLRVYGGLRYTFGG"
     gene            1126..1779
                     /locus_tag="PLY93_09470"
     CDS             1126..1779
                     /locus_tag="PLY93_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69745.1"
                     /translation="MRKLVKKITCALPLMALFALGCAQGGEVASGEVALSTTCESTPT
                     DGFSQVCKPSAGVAKHYRIDGVQTSTAHSSVSLFIGFASQPASGNTNPAAGVGEAKVQ
                     FYHGNGTAPPPQSDVYFANEDITLSPPYTDYTTAAQTICFDITNATPPRITLWATGKN
                     GADCADSAKLTAATAIFNKSDWTTTVALENKNVYVYKATTAAATKVTAYVTTFGTGF"
     gene            complement(1776..2744)
                     /locus_tag="PLY93_09475"
     CDS             complement(1776..2744)
                     /locus_tag="PLY93_09475"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRP69746.1"
                     /translation="MEKGTTKILDWRTVSVEKIFIVMQKYTYFFLLFFIVCTGKNTVV
                     GAGKKKLEIEKSPFGVVSVYDLDGKRYISIGEQEQSAVNLTNKREWVYSYMYLLSLGV
                     LAHERLDGQVPQQVLLIGLGGGSFADFLAEVFPLWQITVVELNPAVIRLAKKYFPLSE
                     KIRIVEGDGRVYLKNTHSQYDVIVMDAFGEHFIPPELYTVEYFRLLKSRVLPQGLVLV
                     NTWEGTPFEMAEVQTLRSVFRTGYYIHHIDETPSNRIYVLGDALPRRKNLQEKIKREF
                     YERHFTGGNPTRFLQSMVDLSTVRTNARVITDRSVRTLFIKRQAVD"
     gene            2786..>3649
                     /locus_tag="PLY93_09480"
     CDS             2786..>3649
                     /locus_tag="PLY93_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69747.1"
                     /translation="MPLFESAFIAGLKSVLTSALYPVLPFLLVLFWHGDKKTRKTRFV
                     HAVIFAFVFTLLFTIMNALGGSGIVRLTANSYFIYAIVFFLLYFALAFARLLPAKITP
                     RFLTQPPTLVSLFALSSFAALFAIAFMGNSVSSLLVQVAKAMQVQTNVLLPLFAVFLF
                     AFAVEFPLLLVSFLLITLPSKPEWQKYMRYAISTLFIAAAFHYAEDSVGNFPDAAQAF
                     PFAAIGFGMCVTFYLLADPALSDASMPRRLKINTSGMLLLSGLGLFLFTSLFVKYWVS
                     KLKPMRSFKKAR"
BASE COUNT          939 a          942 c          787 g          981 t
ORIGIN      
        1 ctattccgcg atcaactgct gtaagaccaa cgcggcgaca ttgcgctcga caactacgaa
       61 gacacccgtg agaacacgta tgtgctccat gcacaaactg cactacctgt tttttcatta
      121 ggccgaatga ctttaggctc agaaagtatt ttcgaggact tgaaatcgcc gcgcgtcggt
      181 ctcgacagga aaaaccgcta ccgcgtcgcg gggtatgcac agttcgattc taaaattttc
      241 gacacactac ttctttcccc cggcattcgt tatgataaag actcacaatt cggcgactac
      301 gtttcacctc gctttggcgc gaagtggggc acggaggatt ttatcctgcg cgcgagtgca
      361 ggtatggggt atcgctcccc ctcgttccgc gagctctatc tctatttcga caacccgtcg
      421 gcgggataca cagtacacgg caacgcatcg ctcaaaccgg aaaattcgca gagcgttaac
      481 gtaggcgtcg agtggtatgc cacacgccgc atttccgtgg acagcaacgc gtactataac
      541 aatctcacaa acctcatcca aaccgccaca gctggcacca ttggtaccat cacccaatac
      601 gcgtataaaa atgtcgcgca cgctttcacc accggcagcg acaaccgcat cacggtttat
      661 ttgacccgtt ttcttcgggc gacgcttggc tattcgtaca cattcgcttg ggataattcc
      721 atccaacgcg aacttgaagg tcgtgcacgt catagaggaa atttaggtct tttattgcgt
      781 taccatcgtc taacgctcca cgcagcactc gcgctcgtcg accgccgtcc ctattacacg
      841 gatgaagaag gcaagccacc cactcaaaca ccaatttatt cacgtgcgta cgccaccgtg
      901 gatgtaaacg gcgaatacgc cttcttaaac ggtctgagta tttttgccgg cggtgaaaat
      961 ttgaactcgg cgggcgatgc agcgtattta ccgctgccgc cactacgcgt ttatggcggg
     1021 ttgcggtaca cattcggggg ataactatga cagactctac accctactct tgataatgag
     1081 tctcattata gattactaaa tttgagaaat ttaaaggaga ataaaatgag aaaattagta
     1141 aaaaaaatta cttgtgcact gccccttatg gcgctcttcg cgttgggctg tgcgcaaggg
     1201 ggagaagtgg caagtggaga ggtggcactt tctacaacct gtgaatcgac accaactgat
     1261 ggatttagcc aagtttgtaa gccatcagca ggtgttgcta aacactaccg cattgatggt
     1321 gtacaaacat cgactgctca ttcatcggta tcgcttttca taggctttgc ttcacaaccg
     1381 gcgtcaggga atacaaatcc tgcagcaggt gtaggcgaag caaaagtgca gttttaccac
     1441 ggcaatggaa cggcgccgcc accgcaatcg gatgtgtatt ttgcaaacga agatataaca
     1501 ctttcacccc cgtataccga ctacacaacg gcggcgcaaa cgatttgctt cgacattacg
     1561 aacgcaaccc caccgcgcat tacactttgg gcgacgggta aaaacggtgc ggattgtgcc
     1621 gacagcgcaa aactcactgc agcaactgcg attttcaata aatccgattg gacaacaacc
     1681 gttgcgcttg aaaataaaaa tgtgtacgta tataaagcga caacggcagc agcgacaaaa
     1741 gtaacggcat acgtcacaac tttcggtacc ggtttttaat cgaccgcttg tcgttttata
     1801 aataaggtac gcaccgatct gtcggtaatc acgcgcgcgt ttgtgcgtac cgtcgataaa
     1861 tccaccatgc tttgtaaaaa gcgggtggga tttcccccgg tgaaatgccg ctcgtaaaac
     1921 tcacgcttta tcttttcctg caaatttttt cgacgcggca aggcatcccc caagacataa
     1981 attcggttac tcggtgtttc atcgatgtgg tggatataat accccgtgcg aaaaacagaa
     2041 cgcaaagttt ggacttctgc catttcaaaa ggagtgcctt cccaagtatt cacgagtaca
     2101 agtccttgag gcaacacacg cgacttaaga agacggaagt attcaaccgt gtaaagctcg
     2161 gggggtataa agtgctcgcc gaaagcgtcc atcacaatca catcgtactg cgagtgcgta
     2221 ttttttaagt acacacgacc atccccctca acgatacgta ttttttcaga aaggggaaaa
     2281 tatttttttg caaggcgaat cacagccgga tttaactcaa ccacggtaat ttgccacaaa
     2341 ggaaatactt ccgctaaaaa atcggcaaag ctgcccccgc ccaaaccgat gagcaacacc
     2401 tgctgtggca cttgtccatc gaggcgttca tgcgccaaca ctcctaagga aagaaggtac
     2461 atgtacgaat acacccattc tcttttattg gttaagttga ccgccgactg ttcttgctcg
     2521 ccaatactaa tgtagcgttt accatcaagg tcgtacaccg agacaacacc gaaaggagac
     2581 ttttctatct ctaatttttt tttacctgcg ccgacaacgg tgttcttgcc tgtacagaca
     2641 ataaaaaata ataaaaaaaa gtaagtatat ttttgcatca caataaaaat cttttctacc
     2701 gagacggtgc gccaatctaa aattttagtc gtgccttttt ccataattat gacgattcag
     2761 ccttcttagc gtatattgtg ggtgtatgcc cctttttgaa agtgctttta ttgcaggctt
     2821 aaaaagcgtt ctcacgagcg cgctctaccc tgttttgcca tttcttctcg tgctcttctg
     2881 gcacggcgat aaaaaaacgc gtaaaacacg gttcgtccac gcggtgatct ttgcctttgt
     2941 ttttactctt ctttttacaa tcatgaacgc tctgggagga agcggcatcg tacgtctcac
     3001 tgcaaattct tatttcatct acgccattgt tttttttcta ctttattttg cgttggcgtt
     3061 tgcacgatta ttgcccgcaa aaatcactcc ccgttttctc acgcaaccgc cgactctcgt
     3121 ttctcttttc gcgctcagtt cttttgccgc actttttgcc atcgcgttta tgggaaattc
     3181 tgttagttct cttttggtgc aagtggcaaa agcaatgcaa gtgcaaacga atgtgttact
     3241 gcctctcttt gcggtatttc tttttgcctt cgcagtggaa tttcccctac tcctggtttc
     3301 attccttttg ataacgttac cctcaaaacc agaatggcaa aaatatatgc gctatgcaat
     3361 cagcactctt tttatcgctg cggcattcca ctacgccgaa gatagcgtgg ggaatttccc
     3421 cgacgctgcg caggcattcc cttttgccgc catcggtttt ggcatgtgcg ttacatttta
     3481 tttactcgct gaccctgcct taagcgatgc ctccatgccg agacggttaa aaataaacac
     3541 ttcggggatg ctccttcttt cagggctcgg tctttttctt ttcacctcgc tcttcgtgaa
     3601 gtactgggtt tcgaagttaa aaccgatgcg ctctttcaaa aaagcacgc
//
LOCUS       DAOMPD010000267         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10502_length_3633_cov_3.080492, whole genome shotgun sequence.
ACCESSION   DAOMPD010000267 DAOMPD010000000
VERSION     DAOMPD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10502_length_3633_cov_3.080492"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLY93_09485"
     CDS             <1..769
                     /locus_tag="PLY93_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69748.1"
                     /translation="EFLYHEPLAHVGGISIDGPKKALVIGGGDGGAAEEFLKYKTIEK
                     VVMVELDGGVVDACKEHIPLISNGAFENPKLELRIEDGIKYVFETKEVFDQVLLDLTD
                     PFGPSIELYTHEFYEQIARILSDKGVLSLHIESPITRPELFAKLYWTLKSVFPIVRPM
                     TNYVPLYGALWGFALASKTTDPTTLDKKTIVERLNAHALPSLKFYNEDTHHSLMALPN
                     YVREMLHQKQEPIHKGDTLPVPAGSKRNLYLFEQEEK"
     gene            989..1657
                     /locus_tag="PLY93_09490"
     CDS             989..1657
                     /locus_tag="PLY93_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69749.1"
                     /translation="MSAIDDAFKAGKDSQKAAELAETEKRLDDQIRQIAERLQAMHTL
                     RDIAPKVKLTPTQTIFATGKDEDGEYIELVAYSFNSQSFNIARPVGTAAKTMRLYFNG
                     KDLTRIKTIVDDQNFFEQYKYYTKASHQNPVKGDPNEIQLQTSFNKPTEIAEKAPDYK
                     VKLGDIENDPTNPNRLKFKRDFYIEHLIYFEKLFRFTFEFQKRGASNGDVETINRMKN
                     SLRY"
     gene            1654..2418
                     /locus_tag="PLY93_09495"
     CDS             1654..2418
                     /locus_tag="PLY93_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRP69750.1"
                     /translation="MIQNSNSIEASSSSNSSKKSFRRQNRQERLLKAKLESLELFVRV
                     IAHDLNGPLTSINLTLQALRRISCSAEVEKLIQVIDRDVLQATSLLEDLKTLNKREVY
                     RLQKINITHEIRQVVERIDLIKNSNIDIHWSTEPHDLYILGDSIRLRRVWHNVITNAL
                     DAIALKKRDEKIRVYVSLKKRNHFVRVQFRDAAGGIAKEYLPRLFEPFYTTKGTKYRG
                     LGLFIARKIIDEHNGKMRLVSRRPYTRVTIDIPVAP"
     gene            2425..3498
                     /locus_tag="PLY93_09500"
     CDS             2425..3498
                     /locus_tag="PLY93_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP69751.1"
                     /translation="MSAPLLNFSGEFKNPLGKILLVEDVESIQLAIRDFLFTRYDVTS
                     VASAEEALSLLAESDDIKLLITDIKLPEMNGLELARLARKLVENIPIILITSYDVNEF
                     IDTIREQEFSQVITKHGRMSLKEIEVTIQKMLTGDIFGVEKYFPDFKIHEIALSEFPR
                     ALANRTLYSIKIKSLQERSVVSERLASQLKNQKKTSEAVIKLVLDEITSNAIFRAPLT
                     DTGEFKYQTKNLHDDILHKHQNIIFEEEDWFTLEFGVYDDWVIITCIDPHGRLTQKEI
                     LFRLHRHLATDAETGLPMGLHDSHGRGLFLLREHLSSVIYNIERGRKTEVICLYNTVA
                     QQAYKNISIYELPVVPTVDEHHE"
BASE COUNT         1126 a          791 c          790 g          926 t
ORIGIN      
        1 cgagtttttg taccatgaac cattagcgca cgttggtggg atttctatcg atggccctaa
       61 aaaagcgcta gtgattggcg gcggcgacgg cggcgccgcc gaagaatttc tgaaatataa
      121 aacaatcgaa aaagtggtga tggtcgaact cgatggtggc gtcgtcgacg catgtaaaga
      181 acatatcccc cttattagca acggcgcgtt tgaaaacccc aaactcgagc tgcgtattga
      241 agatggcatt aaatatgttt ttgaaactaa agaagtgttc gatcaagttc ttctcgactt
      301 aaccgaccct tttggtccgt ccatcgaact ttatacgcac gagttctacg aacaaattgc
      361 aagaatctta agcgataaag gcgtgttgag tttgcatatc gagtcgccta tcacccgccc
      421 cgaacttttt gcaaagcttt actggacgct taaatcggtg ttcccgattg tgcgacccat
      481 gaccaactat gtgccgctct acggtgcgtt gtgggggttt gctttagcga gtaaaacaac
      541 cgaccctaca acattggata aaaaaacgat tgttgaacgg ctgaatgcac acgcgctccc
      601 ttcgcttaaa ttctataacg aagatacgca ccactcgttg atggcgttac ccaactacgt
      661 gcgcgaaatg ttgcatcaaa agcaggagcc tatccacaaa ggagatacac tacccgttcc
      721 cgcaggttca aaacgcaacc tttacttatt cgagcaagaa gagaaataaa aaattgtaat
      781 ttgggatgtg ttcttgtacg agatttttac ataatttacg acattttttt tagaaatgtg
      841 aatccataaa aaaacaggga atggaatttc agtttacgcc accataagtc gatagacaga
      901 ggggaataat agcaaaggga gcagctaaat gccgaatacg atataaaaaa tattcactac
      961 agtaaccata gtttttttca ctgtgtcttt gagcgcaatc gacgatgctt ttaaggcagg
     1021 taaagacagc caaaaagcgg ccgaacttgc agaaactgaa aaacgtttgg acgatcaaat
     1081 tcgtcaaatt gcagaacgac ttcaggcaat gcacacgttg cgcgatattg cgccgaaagt
     1141 aaaattaacg ccgactcaga ctatttttgc cactggtaaa gacgaagacg gagagtatat
     1201 cgagcttgtc gcttatagct tcaactccca atcgtttaat attgcacgac ccgttggtac
     1261 tgccgcaaaa acaatgcgcc tctatttcaa cggtaaggat ttgacccgca tcaaaacaat
     1321 tgtcgacgat caaaactttt ttgagcagta taaatactac acgaaagcat cgcatcaaaa
     1381 cccagtcaaa ggcgatccga atgaaattca attacaaaca tcgttcaata aaccgacaga
     1441 gattgctgaa aaggcgcccg actacaaagt aaaattaggc gatattgaaa acgatcccac
     1501 gaacccaaac cggttaaagt ttaagcgtga tttttatatc gaacatttaa tttactttga
     1561 gaagttgttc cgtttcacgt ttgaatttca aaagcgtggc gcgtcgaatg gcgatgttga
     1621 aacaatcaat cgaatgaaaa actcacttcg gtattgattc aaaacagtaa ttcaatagaa
     1681 gcttcctctt catcaaattc atcaaaaaaa tcgttccggc ggcagaaccg ccaggaacgg
     1741 ttgctcaaag caaagctcga atcgctcgag ctttttgtgc gtgtcatcgc gcatgactta
     1801 aatggtcctt taaccagtat caatctgaca ctacaagcgt tacgccgaat ctcatgttcg
     1861 gcagaggttg aaaaacttat tcaggtgatc gatcgcgatg ttctacaagc gacaagtttg
     1921 ctcgaagatt taaaaacgct caacaagcgg gaagtgtatc gactgcaaaa aataaatata
     1981 acgcatgaaa tacgccaagt tgtcgagagg attgatttaa ttaagaatag taatatcgac
     2041 atccactgga gtacggagcc gcacgatctt tatatattag gcgatagtat tcgcctgcgc
     2101 cgtgtttggc acaatgtaat aaccaacgcg ctcgatgcga tagcgttaaa aaaacgcgat
     2161 gagaaaatac gtgtgtacgt gagtttgaaa aaaaggaatc attttgtgcg tgttcaattc
     2221 cgcgatgcgg cggggggaat cgcaaaagaa tacctgccgc gcctctttga acctttttat
     2281 acgacgaagg gcacaaagta tcgtggtcta ggtcttttta tcgctcgaaa aattatcgac
     2341 gagcataatg gaaaaatgcg ccttgtctcg cgccgccctt atacgcgagt gacgatcgat
     2401 ataccggttg cgccataact aaaaatgtca gcacctctcc ttaattttag cggcgaattc
     2461 aaaaacccac tgggtaaaat tcttctcgtc gaagacgtcg aatcgataca gttggcaatc
     2521 cgcgattttt tattcacgcg ttacgacgta acgtcggtgg ccagcgccga ggaagcgcta
     2581 tcgcttttag ccgagagcga cgacattaaa cttctcatca ccgacattaa actgccagaa
     2641 atgaacggac tggaattggc gcgtttggcg cgtaagcttg ttgaaaatat tcccataatt
     2701 ctcatcacgt cttatgatgt gaacgaattt atcgacacta tccgcgagca agaattttct
     2761 caagtcatca cgaagcacgg gcggatgagt ttgaaagaaa tcgaagtgac gattcaaaaa
     2821 atgctcaccg gcgatatttt tggagtggag aaatattttc ccgattttaa aatacacgaa
     2881 attgcgctat ctgaatttcc acgcgctttg gcgaatcgaa ctctctactc gataaaaatt
     2941 aagtctctgc aagagcgtag cgtggtgagc gagcgtttag cgtcacaact aaaaaatcaa
     3001 aaaaagacat ccgaagctgt cataaagcta gtgctcgatg aaatcacctc gaatgcgata
     3061 ttccgtgcgc cgcttaccga cacaggcgaa ttcaaatacc aaacaaaaaa tttacacgac
     3121 gatattctgc ataagcacca aaatattatt ttcgaagaag aagactggtt cactctcgaa
     3181 tttggcgttt acgacgattg ggttatcatc acttgcatcg atccgcatgg caggctcact
     3241 caaaaagaaa tcttgtttcg gttgcaccgg catttagcta ccgacgcaga aacaggttta
     3301 ccgatgggtt tacacgactc gcatgggcgc ggattgtttc tgctgcgcga acatctctcg
     3361 tcggtaattt acaacatcga gcgcgggcgt aaaaccgagg tcatttgcct ttacaacacg
     3421 gtggctcaac aagcgtataa aaacatttct atttatgaac taccggtcgt accgaccgtg
     3481 gacgaacacc atgaatgaaa aaattatcga gttaaaaaat ctttcgtttt cttacggtaa
     3541 gcggatgatt ctcgacgaca tcaatgtcga gtttaaatcg gagacattta ccgtgattgc
     3601 aggggaatcg ggcagcggga aaagtacgct ctt
//
LOCUS       DAOMPD010000268         3611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10564_length_3611_cov_2.501969, whole genome shotgun sequence.
ACCESSION   DAOMPD010000268 DAOMPD010000000
VERSION     DAOMPD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_3611_cov_2.501969"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..479
                     /locus_tag="PLY93_09505"
     CDS             150..479
                     /locus_tag="PLY93_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69752.1"
                     /translation="MQPVISQLWENLMLFVVIGIFIELAVSAVFSIKILEDFLNTALL
                     RSIKNAIVILAAFGVCAKVDYLRFLYGTKLAMPEVIHYVLSSLVLARLANLFHEFLKY
                     IKTRSLQ"
     gene            610..870
                     /locus_tag="PLY93_09510"
                     /pseudo
     CDS             610..870
                     /locus_tag="PLY93_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
     gene            919..2667
                     /locus_tag="PLY93_09515"
     CDS             919..2667
                     /locus_tag="PLY93_09515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRP69753.1"
                     /translation="MRASQLPLFTSKNDPSDAQVASHRLLSRAGYIRKQSSGLYAYLP
                     FGYMVHRKVEQIVREEMNRFGGVEVSLPVITPAELWQESGRWDIMGKEMMRMHDRHEV
                     GYALGPTHEEAMTDLARSFLQSYKQLPINLYQIGKKYRDEIRPRYGLVRCREFVMKDA
                     YSYHADDASLDETYQKMRECYRAIFDRCGLKTLPVEADSGNMGGSGSEEFMVASEIGE
                     ETLLLCSDDACGYRSNQEKTPYIENRRDILQIVSTPGAIETVHTPDTKTIDDVANLLG
                     VEARHCVKAVIFEDKENIYFCFVPGDRELSEIKMKNITGATDYWAAGEETIQSVTGAD
                     AGFTGPVGLPVSSEHGKQLKKTIHLLFDKHLKNQNGLVVGANQTDHHLKNVCEGRDFT
                     IPDAYDLVVAHDGDTCPQCKKSALTETRGIEVGHIFKLGKKYADKMKLAVLGVDGKPL
                     TPTMGCYGIGVGRTVATAVEQNHDERGIVWNAALAPFQVYLVSLAKGDTEIAYVEALY
                     AKLNAAGVSAYLDDRDERAGVKFNDAELVGFPFIVTVGKKTIESGKVEVQLRRSGEKL
                     ELGEEDLMKLCINRII"
     gene            2721..>3611
                     /locus_tag="PLY93_09520"
     CDS             2721..>3611
                     /locus_tag="PLY93_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRP69754.1"
                     /translation="MFDFTNRVKKIINDYAPREAKRLGHEFLGPEHVLLGLLKAQDSI
                     AVKMLVGLGIDLTELRKVIEKHCEQDGNTLVIDPATKDKMQRILELSRDEARKMRHSY
                     IGSEHILLSLLRDTNGIVASVFQHFQINYQVIRNELNATLGLPQSTTTPPKTTAAQQP
                     KKETSKTPMLDEYARNLSRMAQEKLTDPVIGRDQEIERVVQILSRKTKNNPILIGEAG
                     VGKTAIAEGLAQRIVAQRVPEPMFNKQVFSLDVAALIAGTKYRGEFEDRIKKIMKEVR
                     ISGEVILFIDEVHTIIGAGAA"
BASE COUNT         1015 a          859 c          895 g          842 t
ORIGIN      
        1 ttgattgagt agcgcgtgtg caacatggtc aaaacggcgt tgatcgagcg cggtggggat
       61 tttgaaggta tatgtcataa tgttttggat ttaagagtat agacttaaaa aaatactttt
      121 cataacacgg tcactcgata agatagatta tgcaaccggt tatttctcaa ctttgggaaa
      181 atttaatgct ttttgtcgtc atcggcattt ttatcgagct ggcagtttcg gcggtgtttt
      241 cgatcaaaat cctcgaagat tttttgaaca ccgcactttt acgctcgatt aaaaacgcca
      301 tcgttattct tgcggcattt ggcgtttgcg ccaaagtcga ttatctgcgt tttttatacg
      361 gcacaaagct ggcgatgcca gaggtgattc attatgtgct gtcgagttta gttttggcac
      421 ggctggccaa tttattccac gaatttttga agtatataaa aacgcgtagt ttacagtgaa
      481 cgcttggtgc tcgagataaa aattgggcga taaccgttcc accacatacc atctctatat
      541 gagcactatt aaaattaaac gcgtttacga agccacagac aaaggggatg gttttcgcat
      601 cctcagcgag gactttctaa agaaaaagca cacatcgatc tgtggtataa agacattgcg
      661 ccgacgaatg agttacgcaa gtggtttgcg cacgatgttg aaaaatggcc agaattccaa
      721 aaacgctaca gggcggaatt gactacccac aaagccgcag tcgcagaatt aaaagcaata
      781 ataaagaaag aaaaaaatgt aactcttctt ttctcagcgt ccgatgaagc gcacaacaac
      841 gcggtggtgt tggcgaaagt gatttcataa agaacgctat ttctttacaa cctgtcgaaa
      901 ttactcgact gcacgcgcat gcgcgcatca caactaccac ttttcacctc gaaaaacgat
      961 ccttcggatg cgcaagtcgc ctcgcaccgg ctgctttccc gcgcggggta catccgcaag
     1021 caatcctcgg gcctctatgc atacttgccg tttggatata tggttcaccg caaggttgag
     1081 cagatcgtgc gcgaagaaat gaaccgcttc ggaggcgtcg aagtgtcgtt gcccgtcatt
     1141 acccccgccg agttgtggca agagtcgggt cgttgggata tcatgggcaa agaaatgatg
     1201 cggatgcacg accgccacga agtcggctac gcgttaggac ccacgcacga agaagcgatg
     1261 acagaccttg cgcgcagctt cttacaatcg tacaagcagt tgccgataaa tctctatcaa
     1321 atcggcaaga aataccgcga cgaaattcgt ccgcgttatg gtcttgtacg ttgccgagaa
     1381 ttcgtcatga aagatgcgta ctcgtaccac gccgacgacg cctcgctcga tgaaacatac
     1441 caaaaaatgc gcgagtgcta ccgcgccatt ttcgaccgct gcggattgaa aaccctgccg
     1501 gtcgaggccg actcgggcaa catgggcggc agcggcagcg aagaattcat ggtcgcctct
     1561 gaaatcggcg aagagacgtt gctcttgtgt agcgacgatg cctgcggcta ccgttcgaac
     1621 caagaaaaaa cgccgtatat tgaaaatcgc agagacattc tccaaatcgt ctctactccg
     1681 ggggcaatcg aaactgtaca cactcccgac acaaaaacca tcgacgatgt cgcaaatcta
     1741 ttaggcgtcg aagcgcgcca ttgcgtcaag gcggttattt ttgaagacaa agaaaatatt
     1801 tatttctgtt ttgttcccgg cgatcgcgag ttatccgaaa tcaaaatgaa aaacattacg
     1861 ggggcgaccg actactgggc agcaggcgaa gaaaccatcc agtccgtcac cggcgccgat
     1921 gcaggcttca ctgggcctgt gggattaccc gtgtcgagcg agcacggcaa gcagttaaag
     1981 aaaaccatcc acctcctctt cgataaacat cttaaaaacc aaaacggtct cgtcgtcggc
     2041 gcgaatcaaa ccgaccacca cctgaaaaat gtctgcgaag gtcgagattt caccattcca
     2101 gacgcgtacg atttggttgt cgcgcatgac ggcgatactt gccctcaatg caagaaatcc
     2161 gcgcttaccg aaacacgcgg tatcgaagtg gggcatatct tcaagctggg caaaaaatac
     2221 gccgacaaaa tgaagctcgc ggtactcggt gtcgacggta agccgctgac accgacgatg
     2281 ggctgctatg gtatcggagt cggacgtacc gtcgcgacgg cggtggaaca aaatcacgac
     2341 gaacgcggca tcgtttggaa cgctgcgctt gctccgtttc aggtatacct tgtttcgttg
     2401 gcgaaaggcg acacagaaat agcgtacgtc gaggcgctgt acgcaaaact taacgctgcg
     2461 ggtgtgagcg cgtacctcga cgaccgcgac gaacgcgcgg gggtaaaatt caacgatgcc
     2521 gaacttgtgg gttttccttt tattgtcacc gtcggcaaga aaactatcga gtccggcaag
     2581 gttgaagtgc aactgcgccg cagcggcgag aagttagagc ttggcgaaga agatcttatg
     2641 aagttgtgta tcaatagaat aatttaataa tttgatgact ttttattgac gcgcataccc
     2701 tttggcggta taatatccac atgtttgatt tcaccaatcg ggttaaaaaa attatcaacg
     2761 attatgctcc acgcgaagca aaacgactag ggcatgagtt tttagggccc gaacacgtgc
     2821 tgttgggact actcaaagca caagattcaa ttgcggttaa aatgttggtg ggtttgggga
     2881 ttgacctgac cgaactacgt aaagtgattg aaaagcactg tgagcaagat ggcaacacgc
     2941 tcgtcataga ccctgcaacc aaagataaaa tgcagcggat tttggagctt tcacgcgatg
     3001 aagcgcgtaa aatgcggcat agctatattg gttcggaaca tattttactt tctttattac
     3061 gcgacactaa cggcattgtg gcatcggttt ttcagcattt tcaaatcaac taccaagtta
     3121 tccgcaacga actgaatgca acgttgggac taccgcaatc gacaacgaca cccccaaaga
     3181 ccacagcggc gcaacaacca aagaaagaaa cttcaaaaac gccgatgctc gacgagtacg
     3241 cgcgcaacct ttcgcgcatg gcacaagaaa aactcaccga ccctgtcatt ggccgcgatc
     3301 aagaaattga acgcgtggtt caaattcttt cgcggaaaac aaaaaataat cccatcttga
     3361 ttggcgaagc aggcgtcggt aagactgcga ttgccgaagg gcttgcgcaa cgcattgtgg
     3421 cgcagcgtgt gcccgaaccg atgtttaaca aacaagtttt ctcgctcgat gtggcggcgc
     3481 tcattgcggg cactaagtac cgaggcgaat ttgaagaccg aattaaaaaa attatgaaag
     3541 aagtccgcat cagcggcgaa gtgattcttt tcatcgatga agtgcatacc atcattggtg
     3601 caggggcagc c
//
LOCUS       DAOMPD010000269         3593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10639_length_3593_cov_3.051159, whole genome shotgun sequence.
ACCESSION   DAOMPD010000269 DAOMPD010000000
VERSION     DAOMPD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10639_length_3593_cov_3.051159"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1256
                     /locus_tag="PLY93_09525"
     CDS             177..1256
                     /locus_tag="PLY93_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP69755.1"
                     /translation="MRSLYKAIIAIFFLAPAFGTLYADFFYYPFEFEKQQNDIYALSV
                     EKNILERQLAEAKKDSAFQKNQYEEKIRNLESRIAALTDTLRETQNALRDERRSHVED
                     NAYNTLKIDEKERQIRTLKKESTAAQKKLAEESAAQEKKYLSEIEKLKKELEQNREEY
                     RKELAQLTGQKNERIAELEKALSEKSRELAQCIDLAKQQKQTVSELENQAKELEQKLQ
                     KEIAEGNLRIKRYKDRIVINIDDKILFPSGSASLRREVRATLNTVAEMLVKNTSSRVL
                     IEGHTDNVPIRTPRFPDNWELSSARALSVLRNLLTNKNLDPSRFGAAGYGEFRPVAPN
                     DTAANRQFNRRVDIVLQLANTGEAK"
     gene            1344..2294
                     /locus_tag="PLY93_09530"
     CDS             1344..2294
                     /locus_tag="PLY93_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69756.1"
                     /translation="MDTEIVGLLPNLSPSMTLSEALKEFNSLVAQSKKWCVNPTKEAG
                     LRRNLTPQEQTKFAQNDLALSGLLGCNAWYTMTDRTPEIDIAYTRHTIDPSLSQGVQC
                     VSVGFLQPYILAAHSNCDKLTVVDLSFRTLRLHALMLPLLTAKNFAGIDVVIETWEKQ
                     SGVKLSEVCTSYSLSRCKIELMAFAEKYRSGINVELTLSSLTHFKGDFNQPTIFYSSN
                     ALDPNFTTADEFKKIQANWMTRTAPFYVIYHQAESSNFGVYKLTKQTIQKICADNFIV
                     ARSGHYSKDGCKYFPATRSEYTTYFDNAATNKEEKLPCRF"
     gene            2375..3544
                     /locus_tag="PLY93_09535"
     CDS             2375..3544
                     /locus_tag="PLY93_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRP69757.1"
                     /translation="MKIQNPNMKELSTSAVADVSKAKYKQYGKYLGDFVVGETYEHPR
                     GMTVTAGMIQDFSTTFLEANPLYLNLEYAKALGHKAIPASPMLVFNLLLSLGVQNDSE
                     KAYANLGYYNVKFLKNVYPGDTITSFTKIVDKKERGEGKPGIVTINTVGNNQHGERVA
                     QYMRKIMVPPAPQGYIPPKFIPSKADIPPYAESVEIEIPDFDAGKWPTGVTRPDTYYE
                     DFNVGDIIVSPNGRTITDEHFAWTYRVGNTHPLHFDRLYSQGMSGAMSGDPITYGGLV
                     FAWLLGLAGRDISENALADLGYTEGYHTQPSKSGETVYCIHRVLAKEPVDGKLKAGAI
                     QFQVIGLRDIKPKPAIEKYGADLFIKENNKKDLGKEKIPEKIFEIERRLLIRSRG"
BASE COUNT         1094 a          856 c          819 g          824 t
ORIGIN      
        1 cggcttgaga aagagcagga attacgggaa gccgagttgg gagcgctcat tggccggcac
       61 cagttagaag cggcgcgttt actaaagaag accgacatcg aagcgcggga aaaaacggtg
      121 gtcaaactcg acgaaaaagt tgcggataag caaaaagcgt tagcggaggc aaaataatgc
      181 gtagtctata caaagcgatc attgctattt ttttcttagc gccagcgttt ggtacactct
      241 acgccgattt cttttattat cccttcgaat ttgagaagca gcaaaacgat atttatgcgt
      301 tgagcgtaga aaaaaatatt ttagagcgac aattagcaga agcaaaaaaa gactctgcgt
      361 ttcaaaaaaa tcaatacgaa gaaaaaattc gtaacttaga aagtcgcatt gccgcgttga
      421 ccgatactct acgcgaaact caaaatgcgc tacgcgacga acgccgcagc cacgttgaag
      481 acaatgcgta caacacgctt aaaatagatg aaaaagaacg gcagattaga acattaaaaa
      541 aagaaagcac tgccgctcaa aaaaaattag ccgaagaatc ggcggcgcaa gagaagaaat
      601 atctttcaga aattgaaaag ctcaaaaaag aacttgagca aaaccgcgaa gaataccgca
      661 aagagttggc tcaactcaca ggccagaaaa atgaacgcat cgccgagtta gaaaaagcgc
      721 tcagcgaaaa gtcgcgtgaa ttggcgcagt gtatcgattt agcgaagcaa caaaaacaaa
      781 cggtttcaga attagaaaac caagccaaag agttagaaca aaagctgcaa aaagaaatcg
      841 ccgaagggaa cttgcgcatc aagcgttaca aagatcgcat cgttatcaac attgatgata
      901 aaattctttt cccttctggc tcggcgtcgc tgcgccgcga agtgcgcgca acgctcaaca
      961 ccgtcgccga gatgcttgtt aaaaatacat cctcacgagt gctcatcgaa gggcataccg
     1021 ataacgtccc aatccgcacg ccgcgttttc ccgacaactg ggagctttca tcagcgcggg
     1081 cgctttctgt gttgcgcaat ttactcacga ataaaaattt agacccaagc cgttttggag
     1141 ccgctggcta tggcgaattt agacccgttg caccgaatga cacagcagcc aatcgccaat
     1201 ttaaccgtcg tgtagatatt gtgttacaac tcgccaatac gggagaagca aagtgaaacg
     1261 gaaaacattt tttttactta tatctatttt tgtagcatct tgtacacaag agaaacaagc
     1321 tcagcaaagc attgaaaatt tctgtggata cagaaatagt agggttgttg ccaaaccttt
     1381 caccgtcgat gactttgtcc gaggcgttga aagaatttaa ttctctcgta gcccaaagta
     1441 aaaaatggtg cgtcaatccc accaaagaag cgggattacg ccgcaattta actccacaag
     1501 aacaaacaaa gtttgctcaa aacgatttag cgttaagcgg tcttttagga tgtaacgcat
     1561 ggtacacgat gaccgatcgc acaccggaaa ttgacatcgc ttacacacgc cacacaatcg
     1621 acccaagttt gagccaaggc gttcagtgtg tgtccgttgg gtttttgcaa ccatatatac
     1681 ttgctgcaca ttcaaactgt gataaactca cagtggtcga tttaagtttc cgcacattgc
     1741 gcttacacgc gcttatgttg ccactcttaa cggcaaaaaa tttcgcaggg atcgatgttg
     1801 tgattgagac ttgggaaaaa caaagtggtg taaaactctc agaggtgtgt accagttact
     1861 cgttgagtcg ctgcaaaatc gagcttatgg cgtttgcaga aaaataccgc tcgggtatta
     1921 acgttgaact cacgctttcg tcgttaactc atttcaaagg agacttcaat caacccacaa
     1981 ttttttacag tagcaacgcg cttgatccta attttacaac cgccgatgag ttcaaaaaaa
     2041 ttcaagccaa ctggatgacg agaacggcgc cattttatgt gatttaccat caagcagaaa
     2101 gcagtaactt cggcgtttat aaattaacaa aacaaacgat tcaaaaaata tgtgcagaca
     2161 attttatcgt cgcacgttcc ggtcactatt ctaaagatgg gtgcaaatac ttccctgcca
     2221 cccgcagtga atacaccact tacttcgaca acgccgcaac aaataaagaa gaaaagctgc
     2281 cttgccgttt ttaagagcct gtgcgccaac tcgctgagac tctttagagt tctttcattg
     2341 ttcacaccct gtcttttttt gtaaaaatgc ccttatgaaa attcaaaatc ccaacatgaa
     2401 agaactttcg acgtctgccg tggcagacgt ctccaaagct aaatacaagc aatacggcaa
     2461 gtacttgggc gactttgtcg tcggtgaaac gtacgagcac ccgcgcggca tgaccgtcac
     2521 ggcgggaatg attcaagatt tttcaacgac atttttagaa gcgaaccctc tttacttgaa
     2581 tctcgaatac gcgaaagcgt tggggcataa ggcaatcccc gcgagcccca tgctcgtttt
     2641 caatttactg ctttccttgg gcgtgcaaaa cgattctgaa aaagcgtacg cgaacttggg
     2701 ctactacaac gtgaagtttc tgaagaacgt ctatccgggc gatacgatta cctcgttcac
     2761 caagatcgtg gataaaaaag agcgcggcga aggcaagccc ggtatcgtca cgatcaacac
     2821 cgttggcaac aaccaacacg gcgagcgtgt cgcgcagtat atgcgcaaga tcatggtgcc
     2881 tcccgcgcct caaggttaca taccaccgaa atttattcca tctaaggcgg atatcccccc
     2941 ttacgcagaa tcggtcgaaa ttgaaattcc cgattttgac gcgggtaagt ggccgacggg
     3001 cgtgacgcgc cccgatacgt actacgaaga ttttaacgtg ggcgatatta ttgtttcgcc
     3061 caacggccgc acgattaccg acgagcattt tgcgtggacg taccgcgtcg gcaacacgca
     3121 tcccctgcac ttcgaccgcc tctattcgca aggcatgtcg ggcgcgatga gcggcgaccc
     3181 aattacctat ggcggtctcg tgttcgcgtg gcttttgggg cttgcaggac gcgacatttc
     3241 tgaaaacgcg ctcgccgatc tcggttacac agaaggatat catacacaac cttcgaagtc
     3301 gggcgagacc gtatactgca tccaccgcgt gctcgccaaa gagccggtcg acggcaagct
     3361 caaggcaggg gcgattcaat ttcaagtcat tggcttacgc gacattaaac ccaaacccgc
     3421 gatcgagaaa tacggcgcag atctcttcat caaagaaaac aacaagaaag acctcggcaa
     3481 agaaaagatt cccgagaaga ttttcgagat cgagcggcgg cttttgattc gttcgcgggg
     3541 ttaaaatgcg gagtcggggg tgatttactc gtcgcccatt tgacctgaaa cga
//
LOCUS       DAOMPD010000270         3583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10677_length_3583_cov_2.758220, whole genome shotgun sequence.
ACCESSION   DAOMPD010000270 DAOMPD010000000
VERSION     DAOMPD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10677_length_3583_cov_2.758220"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="PLY93_09540"
     CDS             <1..1505
                     /locus_tag="PLY93_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRP69758.1"
                     /translation="RAESEAARILSGLGLEDALHTLPAQKLSGGQMMRVLIARALFAK
                     GDVLLLDEPTNHLDMPGIKWMEKFLLNEFSGMLLLITPARRFLSDIAEQILDVDYKTI
                     TLFNMPYKRFVGARLLAAEQKEREAEAAKKKIAELTEFIDRFRAKASKARQAQSRAKQ
                     VEKIEIPEIILSSRRKPSFFFRYDREPGRDILNVHNVAMQFTAKPLFTGCEFMLRKGD
                     KAAIIGPNGHGKTTLLKIIAGELKPLEGTVHFGHEVRMGYYAQVHDELRKLSEPMHEH
                     LHELHSTKPVKEVRTALGHMLFEGDDQKKSLSALSGGEMARYVFADLMLQKPNFLVLD
                     EPTNHLDIEGIEALEDAIRDFSGTVLMVSHDRSFIKRACSRVFELKDGALQELNAGVA
                     ALDDVDEEIAPATLARQKAEANTAADTLSYEERKRQRSDAGKLQTQIKKLEAGIAAHE
                     GQLAHLDALFTDGGALQNLSPEDIRAKSAERAQLQKELDAMMSEWGELAG"
     gene            1710..3104
                     /gene="nirK"
                     /locus_tag="PLY93_09545"
     CDS             1710..3104
                     /gene="nirK"
                     /locus_tag="PLY93_09545"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HRP69759.1"
                     /translation="MKLKSVFVMSAALAFLAVGCSKKVEKAVLAHALNVPPQLNRSGN
                     AKVIVNLETKELKGRLADGVEYTFWTFGGTVPGPMIRIREGDDVEFNLSNHPTSKMPH
                     NIDLHAVTGPGGGAAASLTTPGHSSKFSFKALNSGLFVYHCATAPVGMHIANGMYGLI
                     LVEPKGGLPKVDKEFYIMQSEFYTKGKHGQPGLQGFDMDKALDEKPEYVVFNGSVGSM
                     TNEKTLKAKVGDKVRMFVGNGGPNLVSSFHVIGEIFDAVYTEGGAERNQKNVQTTLIP
                     AGGSAIVEFKMEVPGDHVIVDHSIFRTFNKGALGLLKAEGDAAPSIYSGKQSDTVYLP
                     EGGATQTIPSETVVAPGPKNKEERLVAGKRVYDTTCAACHMGQGQGVSKAFPPLAKSD
                     YLNADKKRAIHVVMNGLSGPVTVNKEKYDSIMPALGLSNDDIASVLTYVLNSWGNSGG
                     DITSAEVAAQRGGH"
     gene            3055..>3583
                     /locus_tag="PLY93_09550"
     CDS             3055..>3583
                     /locus_tag="PLY93_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRP69760.1"
                     /translation="MVILLPLKSLLNVVAISKILNYPAHRMRGITIHAMVVAAFFVTP
                     LFAVDMVTIPSGNFRPFDLAKDQKNVKIRRFQIDKYPVSTADFAAFLEKNPAWQKNNV
                     SPIKADKGYLTGVALSPPQSGIAMTQVSWYAARAYCRAEGKRLPTLDEWEYVATYNKW
                     DALPANNERILKWFAQ"
BASE COUNT          965 a          878 c          904 g          836 t
ORIGIN      
        1 accgcgccga atccgaagcc gctcgcattt tgtcggggtt aggtttagag gatgcgttgc
       61 atacgttgcc cgcgcaaaag ctatcgggcg gacaaatgat gcgcgtactc attgcgcgtg
      121 cactttttgc caaaggcgat gtgctgctgt tggacgaacc caccaaccac ttggatatgc
      181 ccggcatcaa gtggatggag aagtttctac tcaacgaatt tagtggcatg ctactcctta
      241 ttacgcccgc ccgccgcttt ctttcagaca tcgccgaaca aattttagat gtggactaca
      301 aaaccatcac tctttttaat atgccgtaca agcgtttcgt gggtgcgcgt cttttagccg
      361 ccgagcaaaa agagcgcgaa gccgaagcgg cgaaaaaaaa gattgcagag ctcaccgaat
      421 tcattgatcg ttttcgtgcg aaagcgtcga aagcaagaca ggcacagtcg cgcgcaaagc
      481 aagtggagaa aattgaaatc cccgagataa ttctctcttc acgccgcaag ccttctttct
      541 tttttcggta tgatcgagaa cccgggcgcg atattttgaa cgtacacaac gtggcgatgc
      601 agtttacagc aaagcctctc tttaccggtt gcgagtttat gttacgcaaa ggcgacaaag
      661 cagcgattat cggccccaac gggcacggta aaaccacgct cttaaaaatc atcgccggtg
      721 agctcaagcc tctcgaaggc acggtgcatt ttggccacga agtacgtatg gggtattatg
      781 cacaggtgca cgacgaactg cgcaagttga gcgagcccat gcatgaacac ttgcacgaac
      841 tgcattcaac aaagccggta aaagaagtac gcaccgcgtt gggacacatg ctctttgaag
      901 gcgacgatca aaaaaaatca ctttctgcgc tttcgggcgg cgaaatggcg cgctatgttt
      961 ttgccgacct catgctgcaa aaaccaaact ttctggtctt ggacgaaccc accaaccatt
     1021 tggacatcga aggtattgaa gcgttggaag acgccatccg tgatttttca ggcacagtgc
     1081 tgatggtgag ccacgaccgt agttttatca agcgtgcgtg cagtcgcgtc tttgaactca
     1141 aagacggtgc attacaagag cttaacgcgg gcgtcgctgc gcttgacgac gtcgacgaag
     1201 aaatcgcccc tgcaacactc gcccgccaaa aagccgaggc caacactgcc gccgacacgc
     1261 tttcttacga agagcgcaaa agacaacgca gcgatgccgg taagttacaa actcaaatta
     1321 aaaaactgga agcgggcatc gctgcacacg aaggccaatt agcccatctc gatgcgcttt
     1381 tcaccgatgg cggtgcatta caaaatcttt cgcccgaaga cattcgggct aaaagcgcag
     1441 aacgcgcaca attgcaaaaa gaactggatg caatgatgag cgaatggggt gagttggcgg
     1501 gatagaaaga agtctgtgcg tcaactcgct ggagcaattt ttttgtgagt ttttgtagac
     1561 gttgcgcgag ttggcacgca ggttcagaga tgaaagtttt tacttgttga ttgatataaa
     1621 tcaatgcaaa ttattttaaa cagtgtttat cctatcaaaa gtgacagcat cgtttacgcg
     1681 ctgtcggcta aaaatttaga ggagagatta tgaaacttaa gtctgtgttt gtgatgagcg
     1741 ccgcacttgc cttcttggcg gtgggttgtt caaaaaaagt agagaaagct gttttggcgc
     1801 atgcactcaa tgtaccacca cagctcaacc gtagcggaaa cgccaaggtg attgtcaacc
     1861 ttgagacgaa agagcttaaa ggccgattgg ccgatggtgt agaatacacc ttctggacat
     1921 ttggcggtac ggtgccgggt ccaatgatcc gtattcgcga aggggacgac gttgagttta
     1981 atctctcaaa ccaccccacg agtaaaatgc cgcacaatat cgacttgcac gcggttacag
     2041 gccccggtgg cggtgcggcg gcttcgctca cgacaccagg gcatagctcg aaattttcgt
     2101 tcaaggcgct caactcaggg ctttttgttt accactgcgc gactgcaccc gtcggtatgc
     2161 acatcgccaa cggtatgtac ggtttgattt tggttgaacc caaaggtgga ttacccaagg
     2221 tagataaaga attttacatt atgcaatccg agttttacac caaaggtaaa cacggccaac
     2281 ctggccttca aggctttgac atggataaag cactcgacga aaaacccgaa tacgttgttt
     2341 tcaacggttc agtcggttca atgacaaacg agaaaacatt gaaagccaaa gtaggcgaca
     2401 aagtgcgcat gtttgtgggt aacggcggtc caaacctcgt ttcctcgttc cacgtcattg
     2461 gtgaaatttt cgacgctgta tacaccgaag gcggcgcaga acgcaatcaa aaaaacgtac
     2521 aaactacgct catccctgcg ggtggttcgg cgattgttga atttaaaatg gaagtcccgg
     2581 gcgaccacgt cattgtcgac cactcgattt tccgtacgtt caacaaaggc gcattgggct
     2641 tacttaaagc cgaaggcgac gcagcaccca gcatttactc gggcaaacaa tccgatacag
     2701 tgtacctacc agaagggggc gcaacgcaaa caatcccgtc tgaaactgtg gttgctcctg
     2761 ggcccaagaa taaagaagaa cgtttagttg cgggtaaacg cgtttacgac accacctgcg
     2821 ccgcttgcca catgggccaa ggccaaggtg tgtctaaggc gttcccaccg cttgcgaagt
     2881 ctgattactt gaatgcagac aaaaaacggg ccattcatgt cgtcatgaac ggtttatccg
     2941 gtcctgtgac tgtgaataaa gaaaaatacg acagcatcat gcctgcactc ggtttgtcga
     3001 acgacgacat tgccagtgtg ttgacctatg tactcaactc atggggtaac tcgggtggtg
     3061 atattacttc cgctgaagtc gctgctcaac gtggtggcca ttagtaaaat tttgaattat
     3121 cccgcgcata gaatgcgcgg gataactatc cacgccatgg tggtggcggc attttttgtc
     3181 accccacttt ttgctgtgga tatggtaaca ataccttcag gtaacttccg ccctttcgat
     3241 ttagcgaaag atcaaaagaa cgtcaaaata cgccgattcc agatcgataa gtatccggtt
     3301 tcgacggcgg attttgccgc ttttttagag aaaaatcccg cttggcaaaa aaataacgtt
     3361 tcgccgatta aagccgacaa gggctatttg acgggcgttg cgctttcccc cccacaatcg
     3421 ggcattgcga tgacgcaagt gtcgtggtac gccgcacggg cgtattgccg cgccgaaggt
     3481 aagcgtttgc cgacgctcga cgaatgggag tacgtcgcca cgtacaataa atgggacgca
     3541 ttacccgcaa ataacgaacg tatcttaaaa tggtttgcac aac
//
LOCUS       DAOMPD010000271         3583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10679_length_3583_cov_2.452381, whole genome shotgun sequence.
ACCESSION   DAOMPD010000271 DAOMPD010000000
VERSION     DAOMPD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10679_length_3583_cov_2.452381"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..2213
                     /locus_tag="PLY93_09555"
     CDS             228..2213
                     /locus_tag="PLY93_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRP69761.1"
                     /translation="MQQGKLNFDIIAEETPLKAVLSHTITSENDSYTLTIRLPQQNKG
                     KIPENLSPYQRLYADLWDLMEQYYREPLAVFSSSRTMLLPHQVEAALKVVSASRPRFL
                     IADEVGLGKTIEAGLVLKELKLKHGYKKILIVVPSPLLAQWQQELKTKFAEEFTILTG
                     DALRRQTAFIDTALPQYLVSVDLAKDERYLETFLAQNFDLVVFDEAHRLRRDANMATQ
                     AWHFANAISRTVDGLLLLSATPFRGKLEEIFFLIQLIDPDILGPLATFQTQFAEDASL
                     LRDRLSPVVIRRRKIDVGGFTKRFAKTVKIDLCKEEREFYDRTTEYVKTEFNRALSRG
                     ENLKTFIMIVFQKLLDSSPYALLKALDGRRARLEGLYFRVMHEMTESEEVAEAIKEFQ
                     EEEGFEESEILNPQEIRAEIQTISHLAKLGQKITHDSKLKQLQKTLKSMGSMGHKKFV
                     IFTQFKSTLEYLLENLKTYKPVGFHGGLNFEQKEEAIRQFFADKDILICTEAGGEGRN
                     LQVAACLINYDLPWSPLKLEQRIGRIHRFGQTRDVHIVNFACRDTVAERVVEVLEEKI
                     RLFESALGPSDTLLGVFESEYKFGRSLMAFLGAKKTRREHNEELERSLFLAKENLRNV
                     DRLLSTEQMNFNLAAFRHARGRALSKATKAAVRWKQS"
     gene            2198..2929
                     /locus_tag="PLY93_09560"
     CDS             2198..2929
                     /locus_tag="PLY93_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69762.1"
                     /translation="MEAIVKEYAAMENLLFKKNGDTVVLENETRRGTFDQEAATKRVD
                     LEFFAFGHELIDRYAHAIISETEKTPIVSIASSENGFLFFFSMSLELDKKYRRMYRVY
                     LSKFGDHEKCPTILSQQAQAHFIDEDYLKLVTQKALARIETKILTDVESILKKIRPSE
                     SYWKNRIVDSAMARKNTIEEKLEIQRSKVKWYGEKFVGSVSRLSSDKRRSEQKEYERL
                     QHSNFRLEPKITLELKRVCVLSSDS"
     gene            2939..>3583
                     /locus_tag="PLY93_09565"
     CDS             2939..>3583
                     /locus_tag="PLY93_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRP69763.1"
                     /translation="MSLLESVKTSVAAAGRVAQTSLVFARHAQFFANVVAHPTAPETA
                     RELRRIFESLGATYIKLGQFIASAPGLFPDAYVTEMQALLDRTTPIEFSELDKILKHE
                     LGTEYRNHFQNIEEIPLASASIAQVHAATLATGESVVLKIQRPNIEAKMDADLGLMSF
                     AARLLEILLPDTKRVGVTDVIHELGRSMADETDFLKEAKNILEFDDFLHQIGEDD"
BASE COUNT          991 a          871 c          856 g          865 t
ORIGIN      
        1 gcccttttag gcctcgataa ttgtgttttg gtggaagaca acggcgtgct tttggtcgca
       61 aaacgcgagg cgttggccga aattaaagag ttgcgcgaaa aagcggcgaa gaatttattg
      121 tgaggggtta aaagcctcag cgagttggcc acaggcctct aacagcacaa gtgtctaaca
      181 gcacaagctc ctttacgcaa taatgcgccc ttgtattatg tctcttcttg caacagggta
      241 aactcaactt cgatattata gccgaggaaa caccgctcaa agcggtgttg agccacacga
      301 ttacctcgga aaatgattcc tacaccctca caatacgatt gcctcaacaa aataaaggaa
      361 aaattccgga gaatctctcg ccttaccaac gcctttacgc cgatttatgg gatctcatgg
      421 agcaatatta ccgtgaaccg ttggcggttt tctcttcgtc acgcacgatg ctgttgccgc
      481 accaagtcga agcggcgctc aaagtggtat cggcgtcccg cccacgtttt ttaatcgccg
      541 acgaagtagg gttgggaaaa actatcgaag cgggtcttgt tctcaaagaa ctgaaactca
      601 agcacggcta taaaaaaatt ttaatcgtcg tgccatcccc cttgctcgcg cagtggcagc
      661 aagagctcaa aacaaaattt gccgaagaat tcactatctt gactggcgat gctctgcggc
      721 gccagacggc gtttatcgac acagcgctac cgcaatactt ggtctcggtc gacttggcga
      781 aagacgaacg gtacttggaa acttttttag cgcaaaattt tgacctcgtg gtgttcgacg
      841 aagcgcaccg cttgcgccgc gacgcaaaca tggcgacgca ggcatggcat tttgccaacg
      901 ccatcagccg caccgtcgat ggtttgcttt tactttcggc gacgccgttt cgcggtaagc
      961 tcgaagaaat tttttttctc atccaactca tcgaccccga tattttaggt cccttggcga
     1021 ctttccaaac acagtttgcc gaggatgctt ccctcctgcg tgatcgttta tcgccggtag
     1081 tcatccgccg ccgcaaaatc gacgtggggg gattcactaa gcgctttgct aaaacggtaa
     1141 aaattgattt atgtaaagaa gaacgcgagt tctacgaccg caccaccgag tatgtaaaaa
     1201 cagaattcaa ccgcgctttg tcgcgcggcg aaaatttgaa gacgtttatc atgatagtct
     1261 ttcaaaaatt attggattcc tctccgtacg cgcttttgaa agcgttggat ggtcgccgtg
     1321 cgcgcctcga aggtttgtat ttccgtgtca tgcacgagat gaccgaaagc gaagaagttg
     1381 ccgaggcgat caaagagttt caagaagaag agggctttga agaaagcgaa attttaaacc
     1441 cgcaagaaat ccgcgccgaa atccaaacaa ttagtcatct ggcaaagttg gggcagaaaa
     1501 ttacccacga tagcaaactc aaacaactac aaaagacgct taaatcgatg ggttcgatgg
     1561 gccataaaaa atttgttatc tttacccagt tcaaatcaac gctggagtat cttctcgaaa
     1621 atcttaaaac atataaaccg gtgggttttc atggcggtct caacttcgag caaaaggaag
     1681 aagctattcg tcaatttttt gccgacaaag atattctaat ctgtaccgaa gcgggaggcg
     1741 aagggcgcaa tttacaagtg gctgcttgcc ttattaacta tgatttacct tggagccctt
     1801 taaaactcga gcaacgcatt gggcgcatcc atcgtttcgg acaaacacgc gatgtgcaca
     1861 tcgtaaattt cgcctgccgc gacaccgtcg ccgaacgcgt cgtcgaagtg ctcgaagaaa
     1921 aaatccgcct gtttgaaagc gcgttaggcc cgtcggatac gctcttgggt gtgttcgaga
     1981 gtgagtataa atttggccgc agcctcatgg catttttggg cgcaaagaaa acgcggcgcg
     2041 aacacaatga agaactcgaa cgcagccttt ttttagccaa ggaaaattta cgtaacgtcg
     2101 atcgcttgct ctccaccgag caaatgaatt ttaacctcgc cgcgttccgc cacgcgcggg
     2161 ggagggcgct ctcaaaggca acgaaagcag ccgtgcgttg gaagcaatcg taaaagaata
     2221 cgcggcgatg gagaatcttc ttttcaaaaa gaatggagac accgttgttt tagaaaatga
     2281 aacgcggcgc ggcacgttcg atcaggaagc ggcgacaaaa cgcgtagatt tggaattttt
     2341 tgcctttggt cacgaactca tcgaccgcta tgcgcacgcc atcatcagcg aaactgaaaa
     2401 aacacctatc gtaagcatcg cttcttccga aaatggattt ctctttttct tttcaatgtc
     2461 gttagaactc gataaaaaat atcggcggat gtaccgcgtg tacttatcta aatttggcga
     2521 ccatgaaaaa tgtcctacta ttttatcaca acaagctcaa gcgcatttta tcgatgagga
     2581 ttatttgaaa ctggtgacac agaaagcgct tgcacgtatc gaaacaaaaa ttttaaccga
     2641 tgtcgaatcc atcttgaaga aaattcgtcc ctctgaaagc tactggaaaa atcgtatcgt
     2701 cgattcagca atggcgcgca agaataccat cgaagaaaag ttggaaatcc aacgcagcaa
     2761 agtgaaatgg tatggcgaaa aatttgtggg ctctgtttcg cgtctctcaa gcgataagag
     2821 acgcagcgag caaaaagaat acgaacgctt gcagcacagc aatttccgcc tcgaacctaa
     2881 aatcaccctt gagttgaaaa gagtttgcgt gttgtcttcg gatagctaaa agactccaat
     2941 gagtctactt gaaagcgtga agacatcggt ggcggcggcg gggcgtgtcg cacaaacgtc
     3001 gcttgtcttt gcacggcacg cgcagttttt cgcgaatgtc gtcgcacacc ccacggcccc
     3061 cgaaaccgcg cgcgagttgc gccgcatttt tgaaagcttg ggggcgacgt atatcaagtt
     3121 aggtcagttt attgcctctg cccccggact ttttcccgac gcgtacgtca ccgaaatgca
     3181 ggcgctttta gaccgcacca cgccgattga gtttagtgaa ctcgataaaa ttttgaaaca
     3241 cgagttgggc actgagtacc gcaaccattt tcaaaatatc gaagaaattc ctttggcgtc
     3301 ggcatcgatt gcgcaggtgc atgcggcgac tctcgcaact ggcgaaagtg ttgttctaaa
     3361 aatccaacgc ccgaacatcg aagccaaaat ggacgccgac ttagggctca tgtcgtttgc
     3421 cgcacgcctt ttagaaatcc ttttacccga caccaaacgc gtcggcgtca ccgatgttat
     3481 ccacgagctg gggcgttcga tggccgatga aaccgacttc ctaaaagaag cgaagaatat
     3541 tcttgagttt gacgatttcc tccatcaaat tggcgaagac gat
//
LOCUS       DAOMPD010000272         3579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10696_length_3579_cov_3.025539, whole genome shotgun sequence.
ACCESSION   DAOMPD010000272 DAOMPD010000000
VERSION     DAOMPD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_3579_cov_3.025539"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLY93_09570"
     CDS             <1..800
                     /locus_tag="PLY93_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRP69764.1"
                     /translation="YPALVTLFLPLFWMFLTALKQEGMGLGTEIIPQGAFGDLYTLKN
                     FTAILGDPDFPFFRFFANSTVVAASAALLSTAVCFMAAYAFARKKFPLKETLYALLMA
                     SMMVPGMIFMVPQFALIADLGWMNTWQALVIPHIANVFGIFLLRQAIERIPRSLFEAA
                     EVDGASDYQTMWLVVVPLVSPILVTLILLTFLGQWSNFLWQLIVNTPDSSWITLPVGI
                     SLFKGQYSIAWEKMMAASVVSVLPVLLLFLFLQRYIISGLTQGGVKE"
     gene            complement(804..2120)
                     /locus_tag="PLY93_09575"
     CDS             complement(804..2120)
                     /locus_tag="PLY93_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRP69765.1"
                     /translation="MYDNRFPRAWITCTLSIPLGFAAVIVAKYLLLLIAIFTNLFFYG
                     RFSIEEILPWQHQLGYFVILMPVVGAFFIGFLARFGSPGIRGHGIPEAMEKILTGESR
                     VPRRITVLKPLSAALAIGSGGPFGAEGPIIATGGALGSWLGQLWPTAESERKILLACG
                     AAAGMTAIFGTPIASIFLAIELLLFEFRTRSFIPVMLAVVTAGSLRSLFFHPEPVFAV
                     PLLHAPNSLSMVLHFISGALYGLVAVGASKIIYIIEDGFEKLPLHWMWWPALGAIAIG
                     VCGFFEVRSLGVGYSNITDTIAGKFGISALFSILIFKFISWAIALGSGTSGGTLAPLL
                     TFGACVGFLFGLLVETQFPNLHFDKHTAALVGMAAVFAGASRAVLASAAFAVEVTGQY
                     HVILPVLGACASAYLVSRALLKYSIMTEKIERRGIPVPHEYFPIKN"
     gene            2342..3019
                     /locus_tag="PLY93_09580"
     CDS             2342..3019
                     /locus_tag="PLY93_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69766.1"
                     /translation="MSPLAERIDSAVEKNQTIKVRAYALLPETEKDLNLIILRLLNKY
                     NKLDLTATLYTCIKELAVNGAKANIKRILFDEVKIDLENEADYRRGLDVFREKLTDKF
                     INEYAQKAKNRGLYVDIKFEYDDTQLSVEVVNNSALTKREDERIREKFRDGLKYENIG
                     ELYMEMQDNSEGAGMGIALILMMLKSENIDPHYFTIYSDYTNKTVAKITFPMSSQFRD
                     ERADFKV"
     gene            3023..>3579
                     /locus_tag="PLY93_09585"
     CDS             3023..>3579
                     /locus_tag="PLY93_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69767.1"
                     /translation="MRHRVAALGKFLLSKKIFSFVIVVNFFSCTFYDNADVELPPEIQ
                     KRLNERVKRIDKEEFLDDTRFQIGKITILRQTVLDSVDFFAPLVNNLHFTTVHSTISD
                     ALNFKEGDIVTRSALYDAERYIRLLDPIKEATILETPNAETGKTDITVVTRDRLSAYV
                     RAGGAGSGGYTSMGVQAGDTSLFGR"
BASE COUNT          998 a          858 c          839 g          884 t
ORIGIN      
        1 agtatcctgc tttagtgacg cttttcttgc cgcttttttg gatgttcctc accgccttaa
       61 aacaagaagg catggggctc ggcactgaaa ttatacccca aggtgcgttc ggcgacctat
      121 ataccctcaa aaattttacc gctatcttag gcgatcccga ttttcctttt tttcgctttt
      181 ttgcgaatag caccgtcgtg gcggcatcgg cggcgctctt gtcgacggcg gtgtgcttta
      241 tggcggcgta cgcgtttgcg cggaaaaaat ttccgctcaa agaaactctc tatgcgctac
      301 tcatggcgtc gatgatggtg ccgggcatga ttttcatggt accgcagttt gcgctcatcg
      361 ccgacttggg gtggatgaac acctggcagg cgcttgtcat cccacacatc gccaacgtgt
      421 tcggtatttt tttgcttcgg caagccatcg agcgcatccc gcgttcgctc tttgaagccg
      481 ccgaggtcga tggtgcgagt gactaccaaa cgatgtggct cgtggtcgtt cctttggtgt
      541 cgcccatttt ggtgacgctc attctgttga cgttcttggg gcagtggtcg aattttttat
      601 ggcaactcat tgtgaacacc cccgactcga gttggattac tttacccgtg ggcatatcgc
      661 ttttcaaggg ccagtattcc atcgcgtggg aaaaaatgat ggccgcctcg gtggtttctg
      721 ttttgccggt acttttgctt tttcttttct tgcagcggta catcatcagc ggcttaacgc
      781 aaggcggcgt gaaagaataa aaattaattc ttgatgggaa aatactcgtg cggtacggga
      841 ataccacgcc gttcaatttt ttctgtcatg attgagtatt taagtaaagc gcgcgacacc
      901 agatacgccg aagcgcacgc tcccaagacg ggtaatataa cgtgatactg accggtaact
      961 tcgacggcaa acgctgccga ggcaagcact gctcgtgagg cgccggcaaa aactgccgcc
     1021 atacccacca atgcagcggt gtgtttgtcg aaatgtaagt tggggaattg cgtttcaacg
     1081 agcaatccga aaagaaaacc gacacaggcg ccgaacgtga gtaatggtgc aagcgtcccc
     1141 cccgatgtac cgctacccaa tgcaatcgcc caggaaataa acttaaaaat taaaatagaa
     1201 aacaatgcag agataccgaa ttttccggcg atggtgtcgg tgatgtttga atagccgacg
     1261 cctaaagagc gtacttcaaa aaaaccgcat actccaattg ctatcgcacc caacgctggc
     1321 caccacatcc agtgtagcgg cagtttttca aaaccgtctt cgataatata aataatttta
     1381 gatgcgccca cagcaaccaa accataaaga gcgcccgaga taaagtggag taccatcgac
     1441 aggctattcg gcgcatggag gagtggtaca gcaaagacag gctcaggatg aaaaaataaa
     1501 gaccgtaagg aacccgcagt cacaacggca agcataaccg gaataaaaga gcgtgtacga
     1561 aactcgaata acaataactc aatcgctaaa aaaatcgatg caatcggcgt gccgaaaatt
     1621 gccgtcatgc cggcagcggc gccacatgcg agtagaattt tacgctcact ttctgcggtc
     1681 ggccacagtt gtcccaacca tgaacccaac gcaccaccgg tggcgataat tggcccttcc
     1741 gcaccgaagg gaccacccga accaatggca agggctgccg aaagaggttt aagtactgtg
     1801 atgcgtcgtg gtactcgact ttctcctgtc aggatttttt ccatcgcttc ggggatacca
     1861 tgccctcgaa tgccgggaga accaaagcgc gccaagaagc cgatgaaaaa tgcaccgacg
     1921 acaggcatga gaataacgaa ataacccaac tggtgttgcc atggcaaaat ctcttcgatg
     1981 gaaaaacgcc cataaaagaa aagatttgta aaaattgcga tgagaagtag tagatacttc
     2041 gcaacaataa ccgcagcaaa tcctaagggg atcgacaatg tgcaggttat ccatgcgcgg
     2101 gggaatcgat tatcgtacat cgtttcaaat tttttttgtt agacactgcg taatataaat
     2161 aaagtattct aagcaggagt ctgtgcgaca actcgcgcag cgtgtacaaa aaatgcctca
     2221 gcgatttccc gcctggccac taagcctcta tctttagacg cttgaatatc caatatgcgc
     2281 acttttccaa acaaaaataa ctttcgcttg tcctttcata ggcgagtcat agtgtaccct
     2341 tatgagtcct ttggcggaac gtatcgattc agcggttgaa aaaaatcaaa cgattaaagt
     2401 gcgtgcgtac gcactcttgc ccgaaactga gaaagacctc aatctcatta ttttacgcct
     2461 cttaaataaa tacaacaaac tcgatttaac cgccacgctc tacacctgca tcaaagagct
     2521 tgcagtcaat ggagcgaaag cgaacatcaa acgcatttta ttcgacgagg tgaaaatcga
     2581 tctcgaaaac gaggcggact atcgccgtgg attagatgtt tttcgtgaaa aattaaccga
     2641 taagtttatc aacgaatatg cgcagaaggc aaaaaatcgt ggattatacg tcgacatcaa
     2701 atttgagtac gacgacaccc aactgagcgt cgaggtggtg aacaatagcg cgctcaccaa
     2761 acgcgaagac gaacgtatcc gcgaaaaatt tcgcgatgga ttaaaatacg aaaacatcgg
     2821 cgagctttac atggaaatgc aagacaattc cgaaggagcg ggtatgggga ttgctctcat
     2881 tttgatgatg ctcaaatccg aaaatatcga ccctcactat tttacaattt atagcgacta
     2941 caccaacaag actgttgcaa aaattacatt ccctatgtcg tcacagtttc gtgatgaacg
     3001 cgccgacttc aaagtatagt acgtgcggca tcgtgtcgcc gcattgggca aatttcttct
     3061 ttcaaaaaag attttctcgt ttgttattgt cgtaaatttt ttttcgtgta ctttttatga
     3121 caatgcggat gttgagctgc cccctgaaat tcaaaaacgt cttaatgaaa gggtgaaaag
     3181 aatagataaa gaagaatttc tcgatgacac tcgttttcaa attggcaaaa ttaccatctt
     3241 aagacaaacc gttttagact ccgtcgattt ttttgcgccg ctggtgaata atttgcattt
     3301 cacgacagtg cattctacca tttctgatgc gctcaatttc aaagaggggg atatcgtcac
     3361 ccgatcggcg ctctacgatg cagagcggta catccgcctt ttagacccta taaaagaagc
     3421 cacaattctt gaaacgccca acgcagaaac gggtaaaacc gatatcaccg tcgtgacgcg
     3481 cgaccgcctc tctgcatacg tgcgcgcggg cggtgcgggg agcggtggct atacttcgat
     3541 gggagttcaa gcgggggaca cgagtctttt cggtcggtt
//
LOCUS       DAOMPD010000273         3570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10740_length_3570_cov_2.591750, whole genome shotgun sequence.
ACCESSION   DAOMPD010000273 DAOMPD010000000
VERSION     DAOMPD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_3570_cov_2.591750"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLY93_09590"
     CDS             complement(<1..750)
                     /locus_tag="PLY93_09590"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002180540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRP69768.1"
                     /translation="MSKILVQKFGGTSVGDPERIKRVAARIQSYYEKGYQLVVVVSAM
                     GDTTDDLIDLAAKISKTPPKREMDMLLSTGEQVSISLLAMALSEIGVPAISFTGGQVH
                     IVTDGNFSNARIASIDTERLSAELNQNHVCIVAGFQGVDEQQNITTLGRGGSDTTAVA
                     LAAALKAKECEIYTDVDGVYTTDPNKVDGARKLAQVSYDEMLELASLGAGVLHSRAVE
                     FAKKYNVVIHVKSSFNFNEGTKIVNENDMMEK"
     gene            847..1224
                     /gene="secG"
                     /locus_tag="PLY93_09595"
     CDS             847..1224
                     /gene="secG"
                     /locus_tag="PLY93_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRP69769.1"
                     /translation="MLDILSTILTVLLVIVSILMIIIILLQSNRSAGGMFGSGAQTAF
                     GGGSADALTKITGGFAAAFLILSFGLAAIKSSQHSSKAAAEHLSKEAAAEAAKTPALN
                     PPTNNAATPAPNAAPAPAAPAKP"
     gene            1244..2200
                     /gene="glpX"
                     /locus_tag="PLY93_09600"
     CDS             1244..2200
                     /gene="glpX"
                     /locus_tag="PLY93_09600"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRP69770.1"
                     /translation="MERNLALEFVRVTEAAALAAARQMGKGDEKIADQAAVDAMRKAF
                     DSVSVTGKIVIGEGERDEAPMLYIGEEVGAGGTPVDIALDPLEGTTITAKGGYNATTV
                     IAVADKGCFLHAPDTYMNKLAVGPAAKGALDITDPIPLTLKRLARALDKYVEDITVCI
                     LDRPRHDDLIREVRAVGSRIKLIGDGDVSGAIATCMDDSPVDILLGIGGAPEGVLSAA
                     ALKCMGGDFQGQLKFRNDDERKRAVEMGISDPDKVFTMEELAKGDSVLFCATGVTDGE
                     MLRGVRFNPKGAVTESIVMRARSRTIRWLKSHHHFDFKPKYL"
     gene            2197..2652
                     /locus_tag="PLY93_09605"
     CDS             2197..2652
                     /locus_tag="PLY93_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69771.1"
                     /translation="MNYAPREEFTSIRVTREGKWFAGAKEIINYKVISHFKQNLFCDE
                     KGVYIHQTFHQFAEKGYIIVEGPLLSVFRIDDTTLTLDSLDTMPINETEVIQNSDDDA
                     LYVFYPRLKCYASVPASVAPDFSRYLEEEGDGFTFLKKPIERKKIQVWL"
     gene            2735..2905
                     /gene="rpmH"
                     /locus_tag="PLY93_09610"
     CDS             2735..2905
                     /gene="rpmH"
                     /locus_tag="PLY93_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRP69772.1"
                     /translation="MKRTWQPANKKRNRTHGFRTRNATAEGREVLRRRRQKGRWKLTV
                     SDERRRWDIVKR"
     gene            2911..3324
                     /gene="rnpA"
                     /locus_tag="PLY93_09615"
     CDS             2911..3324
                     /gene="rnpA"
                     /locus_tag="PLY93_09615"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRP69773.1"
                     /translation="MHPHGLSGVNPPQAYKGRASLFIPLKSKKRIAALFKVGKKIYAS
                     QFLLRYLPTPTTEVDSVVNPPLSVVFAVSHKLGKAHQRNRIKRRLREALAIAVDFHSW
                     RQKKKYDLAILPRVDVATMPFDALVQDLKHALKRL"
     gene            3327..3545
                     /gene="yidD"
                     /locus_tag="PLY93_09620"
     CDS             3327..3545
                     /gene="yidD"
                     /locus_tag="PLY93_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRP69774.1"
                     /translation="MRSFFVFLIRFYQYTFSAVLGGQCRFHPTCSEYAVECFQKHTPH
                     CAFILTLWRILRCQPFAKSGYDPVPEQK"
BASE COUNT          903 a          929 c          867 g          871 t
ORIGIN      
        1 tttttccatc atatcgttct cgtttactat ttttgtacct tcgttaaaat taaagctcga
       61 cttcacatga atgacgacat tgtatttttt ggcaaactcc accgcccggc tgtgtaacac
      121 tcctgcgccc aacgacgcca gttctagcat ctcatcgtag ctgacttgcg caagttttcg
      181 tgcgccatcc accttgttgg gatcggtggt gtaaacaccg tcgacatcgg tgtaaatttc
      241 gcactctttt gctttgagcg cagccgccaa agcaacggca gtcgtgtcgg agccaccgcg
      301 ccccaaagtg gtgatgtttt gttgttcgtc tacgccttga aagccggcga caatacaaac
      361 atggttttgg ttgagctcgg cgctcaagcg ttcggtatcg atggaagcga tgcgcgcgtt
      421 ggaaaaatta ccgtcggtga caatgtgtac ttgaccgccg gtaaagctaa tggcaggcac
      481 gccaatttca gaaagcgcca tcgctaaaag ggaaatagat acttgctcgc ctgtagagag
      541 tagcatatcc atttctcgtt tgggtggtgt ctttgaaatt tttgcagcca aatcgatgag
      601 gtcgtcggta gtgtctccca tcgccgagac cacaacaacc agttgatagc ctttttcgta
      661 gtaactttga atacgcgccg caacgcgttt gatgcgttca ggatcgccga cagaggtacc
      721 gccgaatttc tgaactaaaa tcttgctcat agtgtattaa ctatctccat atcagaaaaa
      781 tgaaacagtg cgtcatgcag aatgtattta cgctcgacgc cctgtcaaaa aagagtgctg
      841 taacctatgt tagatatact ttcgacaatt ttaacggtgc ttttagtcat cgtgtcgatt
      901 ctcatgatta ttatcattct gttgcagtcg aaccgttctg cagggggaat gtttggctcg
      961 ggcgcacaaa ccgcgtttgg tggtggctct gccgatgcgc tgacaaaaat taccggcggt
     1021 ttcgccgcag cgtttttaat tctcagtttt ggtttagccg ctattaaatc ctcgcagcat
     1081 tcgtctaaag cagcggctga acatctcagc aaagaagcgg cggcagaagc agccaaaaca
     1141 ccggcgttga accctcctac aaataacgca gcaacgcctg cacccaatgc cgctccggcg
     1201 ccggcagcac ccgcaaaacc ataggaaata aaggagcatg acaatggaac gcaacctcgc
     1261 gctggaattt gttcgtgtca ccgaagcggc agcgctggct gcggcgcggc agatgggaaa
     1321 aggcgacgaa aaaatcgccg accaagccgc tgtcgatgca atgcgtaaag cgttcgattc
     1381 ggtaagcgtc accggtaaaa ttgttatcgg cgaaggcgag cgcgacgaag cccccatgct
     1441 ttacatcggc gaagaagtgg gcgcaggcgg cactccggta gatatcgcac ttgatcccct
     1501 cgaaggtact acgataacag ccaagggcgg gtataacgcc accacggtaa ttgctgtagc
     1561 cgacaaaggt tgcttcctcc acgcacccga cacgtacatg aataagctcg cggttggccc
     1621 tgcggcaaaa ggcgcgctcg acatcaccga ccccattcct ttaactctca agcgtttagc
     1681 gcgtgcgctc gacaaatacg tcgaagacat cactgtttgt atcttagacc gcccccgcca
     1741 cgacgatttg atccgtgaag tgcgcgcggt gggttctcgc attaagctca tcggtgatgg
     1801 cgacgtttca ggcgccatcg ccacttgcat ggacgattcg ccggtggaca tcctcctcgg
     1861 tatcggtggc gctcccgaag gcgtgctttc cgcagcggcg ctcaagtgca tgggcggcga
     1921 tttccaaggg caactgaaat tccgcaacga cgacgaacgt aaacgtgcag tcgaaatggg
     1981 catcagcgat cccgataaag ttttcactat ggaagaactc gccaaaggcg acagcgttct
     2041 tttctgcgcc accggtgtta ccgacggcga aatgctgcgc ggcgttcgct tcaaccctaa
     2101 aggagcggtg actgaatcga ttgtgatgcg cgctcgctca cgcaccatac gctggttgaa
     2161 atcacaccac catttcgatt ttaagccgaa atatctttga attacgcccc ccgcgaagaa
     2221 tttaccagta tccgcgtgac gcgggagggt aaatggtttg ccggcgccaa agaaattatt
     2281 aactataaag ttatttctca ttttaagcaa aacttgtttt gcgacgagaa aggcgtctat
     2341 atccaccaaa cattccatca attcgccgaa aaaggatata tcatcgtcga aggtccgctg
     2401 ttgtctgtct ttcggatcga cgacacaaca ctcaccttgg atagcctcga caccatgccc
     2461 ataaacgaaa cagaggtcat ccaaaatagc gacgatgatg cgctttatgt attttatccc
     2521 cgcttaaaat gttacgccag cgtgcctgct tccgttgctc ccgatttttc gcgctatctt
     2581 gaagaagaag gggatggttt tacgttctta aaaaaaccaa tcgaacggaa gaaaatccag
     2641 gtgtggcttt gagcctcagc gagttgacgc acagactctt acgcacaact caataaactc
     2701 tttacaacct gttacagact gctaattgta cccaatgaaa cggacatggc aacccgcaaa
     2761 taaaaaacgt aaccgcacac acgggtttcg cacgcgcaat gcaaccgccg aggggcgtga
     2821 agtgctgcgc cgccgccgcc aaaaaggccg ttggaagctc actgtttctg acgaacgccg
     2881 ccgctgggac atcgttaagc gttaatttga gtgcacccgc atgggctctc aggtgtaaat
     2941 ccacctcaag cctataaagg gcgcgcctct ctttttattc cactaaaatc gaaaaaacgt
     3001 atcgctgccc tctttaaagt aggaaagaag atttatgcgt cgcagttttt gttgcgctat
     3061 ttgcccacgc ccaccactga agtcgatagc gtggtgaatc ctccgctctc tgttgttttt
     3121 gcagtaagcc ataaattagg caaagcgcat cagcgtaacc gcataaagcg tcgtttgcgg
     3181 gaagcgcttg cgatagctgt ggatttccat agctggagac agaaaaaaaa atatgatttg
     3241 gcgattttac cccgtgttga cgttgccaca atgccatttg acgctctcgt acaagatctc
     3301 aaacacgcgc tgaagaggct ataatcatga gatcattttt cgtgttttta atccgttttt
     3361 atcaatacac gttctcggcg gttttgggag gccagtgccg tttccatcct acttgttcgg
     3421 agtatgcggt ggagtgtttt caaaagcata caccacactg cgcatttata ctcacgctgt
     3481 ggcgcatctt gcgctgccaa ccttttgcga aaagtggcta cgaccctgtg cccgaacaaa
     3541 agtaacgaag agaataagca tgagccacac
//
LOCUS       DAOMPD010000274         3561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10773_length_3561_cov_2.253565, whole genome shotgun sequence.
ACCESSION   DAOMPD010000274 DAOMPD010000000
VERSION     DAOMPD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10773_length_3561_cov_2.253565"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..406)
                     /locus_tag="PLY93_09625"
     CDS             complement(86..406)
                     /locus_tag="PLY93_09625"
                     /inference="COORDINATES: protein motif:HMM:NF039366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HRP69775.1"
                     /translation="MFQIFSRLLSAKSKTDCSTCPPAPMHDLHSNTSQKKYTLFNAAV
                     DTVKGDFATSEERERRLAICRTCEYLDKGDGKMIRAERCKLCGCFVHIKAAYANASCD
                     INKW"
     gene            complement(410..>1054)
                     /locus_tag="PLY93_09630"
     CDS             complement(410..>1054)
                     /locus_tag="PLY93_09630"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRP69776.1"
                     /translation="LCALLLLSCKKETASDAANKAHREAERTAAILESPDRDEWQKPD
                     AVVEALRLKNGDVVADIGAGTGYFARRFAEKVAPKGVSIGYDINATMVEYMQRDANAR
                     HLVDSYKAHLIGSHNPVLEDNYYDLIFFCNTYQHLENRISYFKSLKKSLKKNGRIVVI
                     DFETLDKTDEESGEVPENLVDKKVVINEFKKAGFTLKQDRNFLPSQYFLEFTRK"
     assembly_gap    1055..1064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1255..>1724
                     /locus_tag="PLY93_09635"
     CDS             1255..>1724
                     /locus_tag="PLY93_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRP69777.1"
                     /translation="MAAGKKAIYYALAANIGIAITKSVAAFFTSSSSMMAEAIHSFTD
                     CGNQILLLLGLRETTAPITPLHPLGHGKASYFWSFIVALLLFLIGGVFSIYEGSHKLS
                     NTKPLEHVWVAILVLSIGIILESFSLYGALKEAQPTQKHRSLFHWFRETRQSEL"
     assembly_gap    1725..1734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1735..2233
                     /locus_tag="PLY93_09640"
     CDS             <1735..2233
                     /locus_tag="PLY93_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69778.1"
                     /translation="IIGEDVAALLGLSIALFFVLLAVITGNPVFDAVGSIAIGVLLIV
                     IATIITFEVKSLIVGESADDEFKKSLDSFIAEKYPNVILINVITQHYGNDIVLAIKAR
                     FKIWPYSAQALVEEINKIEVSLRAQFSAIRFIFFEPDLLPEEKKKKRLQTKKRAKPVK
                     RTKLA"
     gene            2240..3100
                     /gene="lpxC"
                     /locus_tag="PLY93_09645"
     CDS             2240..3100
                     /gene="lpxC"
                     /locus_tag="PLY93_09645"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRP69779.1"
                     /translation="MLNAYRTTIKHPGIYEGVGLHSGILTRLSFYPADCGEGILFFRK
                     GERGKEGAAVKASLDNVIDTSLAVTLGIVGDTDKAFQLQTVEHLLYALFVLGITDIAI
                     EVEGGKEVPIADGSAGPFIQTLQKLEKHEYTQKVKPIVVTHPVSVTDGERYLVAIPSK
                     DFQVSYYIDYPHPLLKNQAVQLRYSDEIFIETIGFARTFGFMKDVEYLRSRGLAQGGS
                     VDNAVIYMPDGTTMNDFRFSGESVLHKILDLIGDLALVGRPIQGHVLGARGGHALDVA
                     FGKKLLAAFG"
     gene            complement(3105..>3561)
                     /locus_tag="PLY93_09650"
     CDS             complement(3105..>3561)
                     /locus_tag="PLY93_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69780.1"
                     /translation="WLHAVATHTFSLVAAWLWQMRPREDTRWRSVFMRHRVSILLYLL
                     AQLTIVLIYAGLDFRFANTAMPWAVGLLQLLLNMAVTALILYNHFGKLRLLRFATALT
                     FSIVNFVLLFDSSFNAFISAEAALAASSFTQIFTLFVFLVYAEEQSEIA"
BASE COUNT          982 a          792 c          776 g          991 t
ORIGIN      
        1 tttcaatcgt tagaaaaggg acttattatt ttgaactcgg cgcatctttt tgtttaagtc
       61 tacaaaacac cgtataaact tttcgtcacc acttgttgat gtcacacgaa gcattcgcat
      121 aggccgcttt gatgtggaca aaacaaccac agagtttgca acgttcggca cgaatcattt
      181 taccgtcgcc tttatctaaa tattcgcatg tgcggcatat cgcaagccga cgctctcgtt
      241 cttcgctggt agcaaaatcg cctttaacag tgtcaacggc ggcgttgaat aatgtgtact
      301 ttttttgact tgtattggaa tgcaagtcgt gcatcggtgc gggagggcag gtcgagcaat
      361 cggttttgct ttttgccgaa agcaagcgtg aaaaaatttg aaacaacgat tatttccttg
      421 taaactctaa aaaatattgt gaagggagaa aatttcgatc ttgtttaagg gtaaatcccg
      481 cttttttgaa ttcgtttatc actacttttt tatcgaccaa attctctgga acctcgcccg
      541 attcttcgtc ggttttatct aaagtttcaa aatcaatcac cacgatgcgt ccattttttt
      601 tcaaactttt tttgagtgac ttaaaatagc tgatacgatt ttctaagtgt tggtaggtgt
      661 tacagaaaaa aattaaatcg taataattat cttctaacac cggattatgc gaaccaatga
      721 ggtgcgcttt gtagctatcc actaaatgac gcgcattcgc atcgcgttgc atatattcaa
      781 ccatggtggc attaatgtcg tagccaattg aaacaccttt tggcgcaact ttttctgcaa
      841 agcggcgcgc aaaatacccc gtgcctgcgc caatgtcagc gacaacgtct ccatttttta
      901 atctcaacgc ttcgacaacg gcatcgggtt tttgccattc atcgcgatcg ggcgattcga
      961 gtatcgccgc tgtacgttct gcctcacgat gcgctttatt tgctgcatcg cttgccgtct
     1021 cttttttgca agagagcaaa agtaaagcgc ataannnnnn nnnngaaaaa gtctgcactg
     1081 caatagtaac gtttcacgac attgattttc ctgagtgggc acacaggttc tatacgatta
     1141 cgtttatcca taagttatag tgacataata cataagtcgt tgccgcctgt cattcaattt
     1201 tagtattgtc gcaatggttt gtgcataacc ataaatcgat gcactgttat tctaatggcc
     1261 gcaggaaaaa aggcaattta ttacgcatta gcggcgaata taggtatcgc tatcaccaaa
     1321 agtgtggccg cttttttcac cagttcgtcg tcgatgatgg cggaagcaat ccattctttt
     1381 accgattgcg gcaatcaaat cttacttctt ttaggcctac gtgaaacaac tgcgcctatc
     1441 acccccttac atcctttagg ccacggaaaa gcttcttatt tttggtcatt catagtggcg
     1501 cttttactat ttttaatcgg tggggttttt tcaatctacg aaggtagcca caaactttca
     1561 aacacaaaac cattagaaca tgtatgggtt gccatactcg tacttagcat tgggattatc
     1621 ttggaatctt tttcgctcta tggtgcatta aaagaagcac aacccacaca gaaacaccgc
     1681 agcctttttc attggttccg tgaaacccgc caaagtgagc tgatnnnnnn nnnncattat
     1741 tggcgaagac gtcgccgcat tattggggct atccatcgcg ctatttttcg tcttgttagc
     1801 cgtcatcact ggaaatcctg ttttcgacgc cgtgggtagt attgctatcg gcgtacttct
     1861 cattgttata gcgacgatta tcacttttga ggtgaaaagc cttatagtgg gtgaaagtgc
     1921 cgacgatgaa tttaagaaat ctctggacag cttcattgca gaaaaatacc ctaacgttat
     1981 actcatcaac gtcatcactc aacattacgg taacgatatt gtcctagcga ttaaagcccg
     2041 cttcaaaatc tggccttact cggcgcaagc acttgtagaa gaaatcaaca aaattgaggt
     2101 ttctctgcgc gcccaatttt cggcaatccg ttttattttc tttgaacccg atcttttgcc
     2161 ggaagaaaaa aagaaaaagc ggctacagac caaaaaacgg gcgaaaccgg taaaaagaac
     2221 taaattagca tgagttttta tgctcaacgc ataccgcaca acaattaaac acccagggat
     2281 ttatgaagga gtgggattac attcgggaat acttacccgc ctttcatttt atcccgctga
     2341 ctgcggcgag gggattctct tttttaggaa aggagaacgc ggtaaagaag gggctgcggt
     2401 taaagcatct ctcgacaacg ttatcgatac atcgctcgcg gtgacattag gcattgtggg
     2461 cgatacggac aaagcttttc aactccaaac cgtagaacat ttactctacg cactttttgt
     2521 gttggggatt accgacattg cgatcgaagt cgaaggtgga aaagaagtcc ctatcgccga
     2581 tgggagcgca ggccccttta tccagacact acaaaagctt gaaaagcatg aatacactca
     2641 aaaggtaaaa ccgattgtgg ttacacaccc tgtatcggtg accgatggcg agcgttacct
     2701 cgtggcgata ccttcaaaag attttcaagt ttcgtattac attgactacc cccatccgct
     2761 tttaaaaaat caagcagtac aactgaggta cagcgacgaa attttcatcg aaacgattgg
     2821 ttttgcgcgc acgtttggct ttatgaaaga tgtggagtat ttacgttcgc gcgggctcgc
     2881 gcaaggcggt agcgtcgata acgcggtcat ttatatgccc gacggcacaa cgatgaacga
     2941 ctttcgtttt tcgggagaat cggtgctgca taaaatttta gatttgatcg gcgaccttgc
     3001 actcgttggt cgccccatac aagggcatgt tttgggggcg cgcggcgggc acgccctcga
     3061 tgttgcgttt gggaaaaaat tactcgccgc gtttggttaa tcttttaggc gatttcactt
     3121 tgctcttcag cgtaaacaag aaacacaaaa agtgtaaaaa tttgggtaaa gctcgacgcc
     3181 gccagtgccg cctctgcact gataaatgcg ttaaagcttg agtcaaaaag caaaacaaaa
     3241 ttgacgatcg aaaaagtgag cgcagtagcg aaacgtaaaa gacgcaactt cccaaaatga
     3301 ttatagagga ttagcgccgt cactgccata tttaagagta actggagaag tccaaccgcc
     3361 cacggcattg cagtgttggc aaagcggaaa tcgagtcctg cgtaaatcag cacaatcgtc
     3421 aactgcgcca aaagatagag aagaatcgaa acgcggtggc gcataaaaac gctgcgccag
     3481 cgtgtatctt ctctcgggcg catttgccat aaccacgccg ccacgaggga aaaagtgtgt
     3541 gtggcgacgg cgtgtaacca c
//
LOCUS       DAOMPD010000275         3549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10808_length_3549_cov_2.583572, whole genome shotgun sequence.
ACCESSION   DAOMPD010000275 DAOMPD010000000
VERSION     DAOMPD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10808_length_3549_cov_2.583572"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLY93_09655"
     CDS             <1..877
                     /locus_tag="PLY93_09655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP69781.1"
                     /translation="GYIGLEIAMAYNRLGVKVRIIEFTDRVLRTQSEDISAELEQAMR
                     SEGIEILPNFRAIKFEKREAEVIIHCKCKDGSMTQIRERGRVLVASGTRPNTAKLGIE
                     TVGLETDASGHIKVNDQMGTNVPHIYAVGDVANTPPFVYTAAYEGRLAVENAFTETVN
                     RADYTALPWVVFTDPQVAGVGMDEMQAAQAGLPYDVSKLELTNLPRAIVANDTRGFIK
                     LIRHRETDRLLGGRIVASEGGELVQSLAFAIKFGITVKQLSEELYPYLTLSEGLKLAA
                     IGFRKDVKKLSCCAS"
     gene            887..1087
                     /locus_tag="PLY93_09660"
     CDS             887..1087
                     /locus_tag="PLY93_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HRP69782.1"
                     /translation="MIKVRRLNGKVFYLNHNLIETIEDTPDTVVKLTNGTKYVVRDGA
                     DALLERVIAFNRQIFAEKMRTE"
     gene            1169..1558
                     /locus_tag="PLY93_09665"
     CDS             1169..1558
                     /locus_tag="PLY93_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRP69783.1"
                     /translation="MTTGKLAEKFKINREAIRFYERKGLLPKPERTATGYRLYDVRAE
                     KTLSFILNSKKLGFTLAEIKSLLSLRIVNGENCSTIRIKAQRKIEDIENKIHQPGSLK
                     KALSLLVESCLKKQTSTYCPIIDNLEN"
     gene            2181..3431
                     /locus_tag="PLY93_09670"
     CDS             2181..3431
                     /locus_tag="PLY93_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRP69784.1"
                     /translation="MSSRSGQRPYKPAVTRHSEYKALWQKHWPDFLAAYPAHNEAKFG
                     KIDTWKINEAKKILSCGQFGRGFQWHECESCKVVLAVPFSCKSRLCLSCYRKRLFGWS
                     IHLAKVLNPALLHFHVVFTMPGQLAELLFTRRINPRFLNLLAARVYAKRQRATAHVDE
                     TFRPGILSTVHLAGNSLNFNPHVHAIATRDLLNPKTGEIREVNFMPYQTIRYDWQRAI
                     CRYLRRRHILSKEEYDYFLKKYAKGFHVYFQKIAGETSDVIFKTAQYIAFGLFHNSQI
                     ESVDHEKSTLTFKFKSHVDAQSREKTFSSLKMPIHEFMARMLFFLPDRHEKSIRYYGI
                     YVRPAKTAALEQSEKLSLWAEGIKSSFDTPNPTACPLCQKNMRTYTIFARDTLRFEKR
                     LRTKYFLADGYFFLKSTRAPPEGQ"
BASE COUNT          978 a          835 c          901 g          835 t
ORIGIN      
        1 aggctatatc gggctcgaaa ttgcgatggc atacaatcga ctcggagtaa aggtgaggat
       61 catcgagttt accgatcgcg tgttgagaac tcagagtgaa gacattagcg ccgaacttga
      121 acaagcgatg cgctcagaag gaattgagat tctcccgaat ttccgggcca taaaatttga
      181 gaaacgcgag gcggaggtaa ttattcactg taagtgcaaa gacggcagca tgacacaaat
      241 cagggaacgg ggcagagttc ttgtggcttc tggtacgcgc cccaataccg cgaagctcgg
      301 cattgagacc gtaggtttag aaacagatgc gagtggtcat ataaaagtca acgaccaaat
      361 gggaacgaat gttccgcaca tttatgctgt gggggacgtc gcgaacaccc caccctttgt
      421 ttataccgcc gcctacgaag ggcgccttgc cgtagaaaat gctttcaccg aaaccgtaaa
      481 ccgtgctgat tacactgcgc tgccttgggt cgttttcacc gacccgcaag ttgccggcgt
      541 tggtatggac gaaatgcagg cagcacaggc cggattaccc tacgatgttt caaagctgga
      601 actcacgaat ctaccgcgtg cgattgtcgc aaatgatacc cgcggcttca tcaagctgat
      661 tcgccaccgg gaaacggatc gcttgctcgg tggccgcatt gtagcatctg aaggcggcga
      721 gctggtgcag tctctggcgt ttgcaatcaa attcggcatt acggtcaaac aattatccga
      781 agaactctat ccgtatctga cgctcagtga agggcttaag cttgccgcca tcggttttcg
      841 caaagacgtg aaaaagctga gctgctgtgc gagttaggca accagaatga taaaggtgag
      901 gcgcctgaac ggcaaagtct tttacctgaa ccataatctg atcgagacga tcgaggacac
      961 accggataca gtggtcaagc tcacaaatgg aacaaagtac gttgtgcgcg acggtgcgga
     1021 tgcattgctt gaaagggtta ttgcgtttaa tcgacagatt tttgccgaaa aaatgcggac
     1081 agaatagtac cgatggtgat gccaacgggt gttatgcggt gaaaaaattg tttgatttgc
     1141 cgcgcgcctt tcacaatggg ggataagtat gacgaccgga aaattagccg agaagttcaa
     1201 gatcaatcgc gaggcaatac gcttctacga acgaaagggc ttactgccca aaccggagcg
     1261 caccgccacg ggttacagat tgtacgacgt gagagcggaa aaaaccttat cgttcattct
     1321 aaactcgaaa aagctgggct tcacgcttgc agaaataaaa tccctgctat ccttacgaat
     1381 tgtaaacgga gagaattgct cgacgatccg tatcaaagcg cagcggaaga ttgaagatat
     1441 cgaaaacaag attcaccaac ccggaagttt gaaaaaagcc ctatcgttgt tggttgaatc
     1501 gtgtcttaaa aaacagactt cgacttactg tccaattata gacaacctgg agaactgaaa
     1561 attgttttgg actctgcatt atagtagtct ggtatatgtg cccgctctgc ggatgaagta
     1621 atcaggtttg cacaggcccg aaagggcagt gtttatcggc agctacttcg ggaaagccag
     1681 atcggtcaag tcgaaaggca tgtgagaagg acattcttat tcgtccgtct aaagtgcgcc
     1741 gccatggagg gcggcggata acaaaggcgc aactatgtca gagaacgcgg aatttacctg
     1801 acgttttgac ttgctgtgtc ttcacggcaa gtcaaaatgt gggtggagcg gctcgtggtg
     1861 gcgcacagaa caagcgtccc gcttgtgcgc catccacgat ctttgccgcg taaccccgag
     1921 cacccgagga gctacggcac cgcagggagc gcagcaggtc aaattttgtt gtctgaagta
     1981 attgcgcctg tgagcccggc taggagggcc gggcgactag gattgcgctt tcataacaac
     2041 gaaaaatgcc catattgatg tgaatatcgt tttttttgag ttattgtgaa gatttctgtc
     2101 tgaattgtgg gaaaattcgg ctcatttctg gtatttaata tatagagggt ggatgatgct
     2161 ctccggagca attcatctac atctcatcga gatcaggtca gcgaccgtac aagccggcgg
     2221 tcacgcggca cagtgaatac aaggcgcttt ggcagaagca ttggcctgat tttcttgcgg
     2281 cctacccggc gcacaatgag gctaaattcg gtaagattga cacatggaaa attaacgaag
     2341 ccaaaaaaat cttaagctgt ggtcagtttg ggagaggctt ccagtggcac gaatgcgaat
     2401 cgtgcaaagt ggtcttggcg gtaccgtttt catgcaaaag tcggctctgc ctttcatgct
     2461 accgcaaacg gttattcggt tggtcgattc atttagccaa ggtgctgaat ccagcgttac
     2521 tgcattttca cgtagttttt accatgccgg ggcagctcgc ggaattgctt ttcactcgac
     2581 gaatcaaccc gcgctttttg aatcttcttg cggccagagt ttacgcgaaa cggcagcgcg
     2641 cgaccgcgca cgtagatgaa acttttaggc caggaatctt gagcaccgtt cacctggctg
     2701 gcaacagtct gaatttcaac cctcacgttc atgccatcgc cacacgcgat ctcttaaacc
     2761 ccaagaccgg agaaattaga gaagttaatt tcatgcccta ccagaccata cgttatgact
     2821 ggcaaagggc aatttgccgc tacctacgtc gacgacatat actatcgaaa gaggaatatg
     2881 attattttct aaaaaagtac gcgaagggat ttcacgtgta ttttcagaaa atcgccgggg
     2941 aaacgagcga cgtcattttc aagactgcac aatacatcgc gttcggtctc tttcacaaca
     3001 gccagatcga aagcgtagac cacgaaaaga gcacgctgac atttaaattc aaaagccacg
     3061 tagacgcgca aagccgggag aaaacattca gttcgttgaa aatgccgatc catgagttca
     3121 tggcgcggat gctttttttt ctgccagacc ggcatgagaa atcaatacgc tattatggca
     3181 tctacgtacg cccggcgaaa actgccgctc tcgaacagtc tgaaaaactt tccctctggg
     3241 cagagggtat caagtcttca tttgacacac cgaatcctac ggcctgtccg ctatgccaga
     3301 aaaatatgcg aacatacaca atatttgcgc gggacacact tcgcttcgag aaacgccttc
     3361 ggacaaagta ttttctcgct gacggctact tttttctgaa atcgaccaga gcaccgcctg
     3421 aagggcaata aatttcgtaa atcgtatcaa atagccattt ttttaacgca tcaacactgc
     3481 ggcggggatg ttacacgcca tgcgccaaaa aaccaataaa aacgccgttt tcggccgcaa
     3541 atatagtcc
//
LOCUS       DAOMPD010000276         3548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10811_length_3548_cov_2.688520, whole genome shotgun sequence.
ACCESSION   DAOMPD010000276 DAOMPD010000000
VERSION     DAOMPD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10811_length_3548_cov_2.688520"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PLY93_09675"
     CDS             complement(<1..1176)
                     /locus_tag="PLY93_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP69785.1"
                     /translation="MNLRANNISKLQKIHFDALVVGGGINGAVSAAALSGKGAKVALI
                     DRKDFASETSQESSNLAWGGIKYLESLEFGLVRKLCKSRNHLIKSYPSSVQEIRFFTT
                     IEKGFRFPVWFIWLGTWFYWLIGSFFTRRPRYLSRRRIQKDEPIINTANAVGGFEYSD
                     AYLHDNDARFVFSFVRSAMNNGCIAVNYLEALKATRSHNQWHVDAIDTISGKKLKITA
                     DVIVNACGPYADIFNTHTGVETKHQHVFSKGIHLVVNRLSKETRVLTFFADDGRLFFA
                     IPMGAKTCIGTTDTRMPSPEARVTEEDRDFVLGNINKRLKLDKPLTRADIIAERCGVR
                     PLVTKRNTDGGKDWMQMSRKHEVEANFDTQYITIFGGKLTDCLNVGDEVSELVENMGI
                     "
     gene            complement(1479..2327)
                     /locus_tag="PLY93_09680"
     CDS             complement(1479..2327)
                     /locus_tag="PLY93_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRP69786.1"
                     /translation="MIINHNMSAINTHRVLKFQNWNLDKDMEKLSSGMRINRGGDDAS
                     GLAVSEKMRTQVNGLRRAEQNTEDGMSFIQTTEGYLQETGELVQRIRVLAVQAANGIY
                     TDEDRQQIQVEISELIDEIDRIASQAEFNKMKLLTGSFARLNPTASMWFHMGANMHQR
                     ERVFIETMTSAALKLRNPTVLTFISISTPGKANSVIGLSDEALRIISKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDTDMAETMSSYTRYQILTQAGTAMLAQANQRPQS
                     VLQLLR"
     gene            complement(2636..>3038)
                     /locus_tag="PLY93_09685"
     CDS             complement(2636..>3038)
                     /locus_tag="PLY93_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69787.1"
                     /translation="NYTATGKVTVNGDTTLAIDDTKLVQSQKYTLNFITGTTWRFKCY
                     ITDSNITSYTSTTPLVSIYTQETGADGLTTGRGGNDNLLAPKGYNYFCYADTNGGGNY
                     FESGTDKLAVGVLPSSIGDMITVPLTQFQDF"
     assembly_gap    3039..3074
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3075..3532)
                     /locus_tag="PLY93_09690"
     CDS             complement(<3075..3532)
                     /locus_tag="PLY93_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69788.1"
                     /translation="MRFAGRWWIVIPAALWAFLFTACIQTTELLQPVNSKPITTAAQG
                     TLTLQLNLTATEHKNQVFSVLVFQMGTLKAVLTDDGSATTDGAGAASVAVKAVDTNNC
                     ATATNALLDNGEYNYYFVINYNTLARTAVNTGATGNGCGASGFIDSTAGGT"
BASE COUNT          829 a          832 c          873 g          978 t
ORIGIN      
        1 aatccccatg ttctcgacca actcggacac ctcatcaccc acgttcaaac aatcggtgag
       61 cttgccgcca aagatggtga tgtactgcgt gtcaaaattt gcctcgactt cgtgcttgcg
      121 tgacatctgc atccaatctt tgccgccgtc ggtgttgcgt ttggtcacca aggggcgcac
      181 accgcaacgt tcggcaatga tgtcggcacg ggtgagcggt ttatcgagtt ttaatcgctt
      241 attgatgttc cctaaaacaa aatcgcgatc ctcctcggtg acgcgcgctt cgggagatgg
      301 cattcgcgtg tcggttgtgc cgatgcaagt ttttgcgccc atggggatgg caaaaaacaa
      361 acggccatcg tctgcaaaaa aagtgagcac cctcgtttct ttagagaggc gattgacaac
      421 aaggtgaatc cctttagaaa agacatgttg gtgttttgtt tcaactcccg tgtgtgtgtt
      481 aaaaatatct gcgtacggac cgcacgcgtt cacgataaca tctgccgtga tttttaattt
      541 cttcccggag attgtatcga tggcatccac gtgccactgg ttgtggctgc gcgttgcctt
      601 gagagcctct aaataattaa ccgcgatgca accgttgttc attgccgaac gcacaaatga
      661 aaaaacaaac cgagcatcgt tgtcgtgcaa gtacgcgtcg gagtattcaa agccacccac
      721 agcgtttgca gtgttgatga tcggttcatc cttttggatg cgacggcgcg aaagatagcg
      781 ggggcgacgg gtgaaaaagc taccgatgag ccaatagaac caagtgccga gccaaataaa
      841 ccacaccgga aaacgaaacc ctttttcgat ggttgtaaaa aagcgaattt cttgcaccga
      901 cgaaggatag cttttaatga gatggttgcg cgatttgcac aatttacgca ccaaaccaaa
      961 ctcgagtgat tctaaatact taattccgcc ccacgcgagg ttcgatgact cttggcttgt
     1021 ttcggatgca aagtccttgc gatcgatgag cgccaccttt gcgcctttgc cggagagcgc
     1081 cgctgccgaa accgcgccat taatgccacc gcccacgacg agtgcatcga aatggatttt
     1141 ttggagttta gagatgttgt ttgcgcgtag gttcatagtg agtaaggata ttatttacta
     1201 tactccactt aagcgtgaac atagtgcggc gagcacaatt caaccggtat aatttaataa
     1261 attggataac tgcctttata aaaaaaggtc gaggctaacc tcgaccttca gagtcgtacg
     1321 gcgaagggct tggagccctt aggtttgcgg ccgacacgat gtcgtttttg ccgcaaaccg
     1381 aagtactcaa gttctcctgc cactggtttg atttttttta tgccgcactt ggtgcggcat
     1441 aaaaaaagta ctcagttaat actgagtact tcgatctctt aacgaagaag ttgcaacacg
     1501 ctttgcggac gttggttcgc ttgcgcgagc atcgctgtac cggcttgggt gagaatttga
     1561 taacgagtgt aactcgacat cgtttccgcc atgtcggtgt cgcggatacg gctttcagac
     1621 gcttggatgt tttcgtaagc gttcatgaga cctttcgctg catgctcaag acggttgtag
     1681 tacgcaccta agtcggcgcg ttgcttgctg atgatgcgca acgcttcatc agacaaacca
     1741 atcactgagt ttgctttgcc cggtgtggaa atcgagatga aggtcaacac tgttgggtta
     1801 cgcaatttta acgcagctga tgtcatcgtt tcaataaaca cacgctcgcg ttggtgcata
     1861 tttgcgccca tgtggaacca cattgaagcg gtggggttta agcgtgcaaa tgaacctgtt
     1921 aaaagtttca ttttgttaaa ctcggcttgc gatgcgatgc gatcaatctc gtcgatgagt
     1981 tcggaaatct ccacttggat ttgttggcgg tcttcgtcgg tgtagatacc gttggccgct
     2041 tgaactgcga gaacgcggat gcgttgtacc agttcgccgg tttcttgaag atagccttct
     2101 gtggtttgga taaagctcat accgtcttcg gtgttttgtt ctgcacgacg cagaccgtta
     2161 acctgtgtac gcattttttc agatactgca agacctgacg cgtcgtcgcc gcctcggtta
     2221 atgcgcatac cgctcgaaag cttttccatg tctttatcta aattccagtt ttggaatttc
     2281 aacacgcggt gcgtgttgat ggcagacata ttgtggttga taatcatttg tttcctccgt
     2341 gaaactcttt ttgcccggtg gcggctttta acctaacccg cgagacctca tgtctcgccg
     2401 aggttatctt atcggttgcc tatatggttc gtatcgcgac ccgcctttgg gtctctctac
     2461 gagaggcagc cgaggtgaaa caacctctgc ccagtcttac atttcatatc ggcggattaa
     2521 atttttactt aatagagttt tataaaaaag cgatgcactt ttttttattt ttgaaaaaaa
     2581 gtgctcaagc gatttgtgaa ttgtgccgac ggcgcttctt caagtaacag atagattaaa
     2641 aatcttggaa ttgtgtcaac ggaactgtaa tcatatcacc gatggaactg ggtaaaacac
     2701 caacagccag cttatccgtc cccgattcaa aatagttgcc gccaccgttc gtatcggcgt
     2761 agcaaaagta attataccct ttgggtgcaa gcagattgtc gttgccgcca cggcctgttg
     2821 tcaaaccatc cgctcctgtt tcttgcgtgt atatggatac gagcggtgta gtggaggtat
     2881 acgacgttat attgctatct gtaatgtaac acttgaaacg ccaagtggtg cctgttataa
     2941 aatttaacgt atatttttgg ctttggacta atttggtatc gtcgatggcg agcgtggtgt
     3001 cgccgttcac ggtcactttc cccgttgcgg tatagtttnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnngtgccc ccggcggtgg agtcgataaa gcccgatgca ccgcaaccat
     3121 tccctgttgc acccgtgttc actgcggtgc gcgccagagt gttgtagtta atgacaaaat
     3181 aataattata ttccccgttg tccaagagcg catttgtcgc cgttgcacaa ttattggtgt
     3241 ccaccgcttt gacggccaca ctcgccgccc ctgcaccgtc ggttgttgca ctgccatcgt
     3301 cggtgagcac cgccttgagc gtacccattt gaaacaccaa cacgctaaag acctggtttt
     3361 tgtgttctgt ggcagttaag ttgagctgga gtgtcagtgt gccttgtgct gcggttgtga
     3421 tgggtttact attcactggc tgcaaaagtt cggtggtttg gatacatgcc gtgaacaaaa
     3481 aagcccaaag ggcagcgggg ataactatcc accaacggcc agcgaagcgc attaaggtaa
     3541 ccctctca
//
LOCUS       DAOMPD010000277         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10840_length_3541_cov_2.417958, whole genome shotgun sequence.
ACCESSION   DAOMPD010000277 DAOMPD010000000
VERSION     DAOMPD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10840_length_3541_cov_2.417958"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLY93_09695"
     CDS             complement(<1..671)
                     /locus_tag="PLY93_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP69789.1"
                     /translation="MSLVVLLLAFSPLVFALFLFLFGIKSERILSPLLQVFNALYSAL
                     TIILLALYILSHFQDTPIDVLTVFQAGSYSFKLQFLIDHYSIACQTLIGLLSGLVFMF
                     SRRYMHREPGYRRFFISLLIFRSGISMVALANNISVLYAGWEMVGLSSFLLIGFYQTR
                     KGSGRHALRVFTIYRFCDVGLFIGAWLFHELGGEFTFFDFNTVDKRNIPLLASQSAAF
                     TLIGF"
     gene            complement(668..3157)
                     /locus_tag="PLY93_09700"
     CDS             complement(668..3157)
                     /locus_tag="PLY93_09700"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HRP69790.1"
                     /translation="MGIDLGGLARYLPTQGPIRNFIHHNTLAEFQKEGYTFHEALRAA
                     ADLYGTKTYFPPDEYIRLYGKKEITDVAIERVLKREKLPRETKTALFDLRNYRDESPS
                     AFKQRGILWFLGERHGIHIDEMLNPVVVRIVANYLDQGVLSLRFADHANTFWQTLQAQ
                     LSTASLAGVHKKSRALIIENNADALVEELLIRFVPNEKYQAAFVLEVLLELSGWAGLT
                     HQLEKNPTALFSARPITLLEFVALYLALLVGFTPNATLSIPEGYTPPALLSHEAPAEN
                     KSMQLRRLWHEAWELSHAMGMMAAMRDKSHRQKIRSSRAQDAKVQAFFCIDDRECSLR
                     RLVEEFSFDIETFGTAGHFAIDAYYLGPHSTTPTKQCPGPVDALHVVRGTLKGKKKAT
                     ALSQREMSFWHRYANHLYSGWLLSYAFSFLALGRLLVSVFFPRKNFATAAAIMNAEGH
                     ATLHYEKTKGTPEGYTVQEMAARVKSVLTEVGLIHSFAPLVVLVGHGSTSTNNPYFAA
                     YNCGACSGHAGAVNARVFAAMANRSDVRALLKKDAILLDDDTFFIGALHDTARDEIHF
                     FDTDLLPSRLKSHFEKTKQLFSVALEENARERSRRFAMVPLKISAKRAHNEAKLRTDM
                     LFEPRPEYNHANNSMLVIGRRLVTENLFADRRLFLNSYNPFADAQGVVLEKIIAAAVP
                     VCAGINLEYLFSRLDAEIFGAGSKLPQNIISLLGVAIGSEGDLRTGLPTQMTEIHDPL
                     RLTVIIEQSESVVTKIFEKNTAFKELVFGGWIYLALYDASRGRYIIFEKNKFVPIDVS
                     KVPVPIKQSSVEITHGTRENILPTRLMRAKA"
     gene            complement(3273..>3541)
                     /locus_tag="PLY93_09705"
     CDS             complement(3273..>3541)
                     /locus_tag="PLY93_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69791.1"
                     /translation="LIGMGILPLHFKNGENAQTLGLDGTEVFNLEGYNNAMKPHTDIT
                     VTAKRADGSIVTFVTKNRADTPVEVVYLKNGGILHTVLRKLGTT"
BASE COUNT          950 a          840 c          867 g          884 t
ORIGIN      
        1 aaaaaaccga tgagagtaaa cgctgccgat tgagacgcca aaagcgggat atttctttta
       61 tcgacggtat tgaaatcgaa aaaagtgaac tctccgccca actcgtggaa aagccatgcg
      121 ccgataaaga gacccacgtc acaaaaacga taaatggtaa agacacgcaa tgcatgacgc
      181 cccgaaccct tgcgagtttg ataaaaacca attagaagaa atgacgaaag gccgaccatt
      241 tcccatcctg cgtacaatac gcttatattg ttcgccaaag caaccattga aatgcccgaa
      301 cgaaaaatta agagcgagat aaaaaaacga cggtacccag gctcgcggtg catatagcgt
      361 cttgaaaaca taaagacgag ccccgacaaa agtccaatga gtgtttggca tgcgatggaa
      421 taatggtcta tgagaaattg taatttaaaa gaataactcc ctgcttgaaa aactgtcagc
      481 acgtcgatgg gagtgtcttg aaaatgcgag agaatgtaga gagcgagaag aataatcgtt
      541 aaagcactat agagtgcatt aaaaacttgt aataacggcg aaaggatgcg ctcgcttttt
      601 ataccgaata aaaataaaaa aagcgcgaag acaagcggag aaaaagctaa aagtaaaaca
      661 acgaggctca cgcttttgcc ctcatcaatc ttgtcggtaa aatattctcg cgtgtcccgt
      721 gtgtgatttc aaccgacgat tgttttatgg gcaccggaac tttggataca tcgattggca
      781 caaatttatt tttttcaaaa atgatatagc gacctctgga ggcatcgtag agcgccaaat
      841 aaatccaccc cccaaaaacg agctctttga atgcggtatt cttctcgaat atcttcgtca
      901 caacgctttc gctttgttcg ataatcaccg tcaagcgcag cggatcgtgg atttctgtca
      961 tttgcgtagg aagccccgtg cgtaaatctc cctctgaccc gatggcaacg cccaagagcg
     1021 aaataatgtt ttgtggcagt ttagaacccg cgccgaaaat ttcagcatcg aggcgcgaga
     1081 agaggtactc caaattgatg cccgcacaca ccggcacagc ggcggcaata attttctcca
     1141 gcactacgcc ttgggcgtcg gcgaatgggt tgtacgaatt caaaaaaagt cggcggtcgg
     1201 cgaataaatt ttcggtgaca agccgccgcc caatcacaag catcgaattg tttgcgtggt
     1261 tatactcggg gcggggttca aaaagcatgt cggtgcgtag ttttgcttcg ttgtgcgcac
     1321 gtttggcgct aattttcaaa ggcaccatag caaaacggcg gctgcgttcg cgggcatttt
     1381 cttcgagcgc aacagaaaaa agttgttttg ttttttcaaa gtgggatttc aaccgagacg
     1441 gtaaaaggtc cgtgtcaaaa aaatggattt catcgcgcgc ggtatcgtgc agtgcgccga
     1501 taaagaatgt atcgtcgtcg agcagaatgg catctttttt gagaagcgca cgcacgtcgc
     1561 tgcggtttgc catcgccgca aaaacgcgcg cgttgaccgc acccgcatgg ccgctacacg
     1621 cgccgcagtt gtacgcagcg aagtaagggt tgtttgtgct tgtggagcca tgccccacta
     1681 aaacaaccag cggcgcgaag gagtggatca atcccacttc ggtaagcacg ctttttacac
     1741 gcgcggccat ctcttgcacg gtgtatccct cgggagtgcc ttttgttttt tcgtaatgaa
     1801 gcgtggcgtg tccctcggcg ttcatgattg ccgcagcggt tgcaaaattt ttgcgtggaa
     1861 aaaaaacaga aactaaaaga cgtcccaacg ccaaaaaaga gaaagcataa cttaaaagcc
     1921 aacccgagta taaatggttt gcatagcggt gccaaaaact catctcgcgt tggctgagtg
     1981 ccgttgcttt cttctttccc ttgagcgtgc cgcgtacaac atggagcgcg tcaacaggcc
     2041 ctgggcactg tttcgtgggt gtcgtgctgt gcggccctaa ataatacgcg tcaatcgcaa
     2101 aatgccccgc tgtaccgaaa gtttcaatat cgaaagaaaa ttcttccacc aatcggcgca
     2161 acgagcattc acggtcgtcg atgcaaaaaa atgcctgtac cttcgcatct tgcgcgcggc
     2221 tcgaacgaat tttttgccgg tgcgatttat cgcgcatcgc agccatcatt cccattgcat
     2281 gactgagttc ccacgcttca tgccacagac ggcgtagttg catactttta ttttctgctg
     2341 gcgcttcatg cgacaagagc gcaggtggtg tgtagccttc aggtatcgaa agagtggcat
     2401 tgggcgtgaa tcccactaag agcgctaaat agagcgcaac aaattcaaga agcgtaatgg
     2461 ggcgtgcaga aaataatgca gtgggatttt tctctaactg gtgcgtaaga cctgcccaac
     2521 cggaaagctc gagcaacacc tcgagtacaa atgcggcttg gtatttttcg ttgggtacaa
     2581 atcgtataag tagctcttct acaagagcat ccgcattgtt ttctataatg agtgcacggc
     2641 tttttttatg cacgccagcc agagacgccg tcgagagctg cgcttgcagc gtttgccaaa
     2701 aagtgttggc gtggtcggcg aagcgcaaac tcaatacgcc ttggtcgagg tagttcgcta
     2761 caatgcgtac cacaacgggg ttcagcattt catctatatg gataccgtgc cgttcgccca
     2821 aaaaccaaag tatcccgcgt tgtttgaatg cgcttgggct ttcgtctcta taatttcgta
     2881 aatcaaaaag tgcggtcttt gtttcacgcg ggagtttttc gcgcttcaaa actctttcta
     2941 tcgcaacgtc ggtgatttct tttttcccat aaaggcggat gtactcgtcc ggcgggaaat
     3001 acgtttttgt tccgtataaa tccgccgccg ctctcagtgc ctcgtggaaa gtataacctt
     3061 ccttttggaa ttcagcaaga gtgttatggt gtataaaatt gcgtatcggc ccttgcgttg
     3121 gtagatagcg cgccaaacct ccgaggtcta tgcccatggt ttattaaagc agccagtgcg
     3181 tttcactctt gcaacaccga gggatttttg tatctcgtca aggaaagtgg ggcgattgga
     3241 cagcctccaa taccccactg tattcaagta ttttaggttg tacccagttt ccgaagcacc
     3301 gtgtgcaaaa taccaccgtt cttgagatat accacctcaa cgggtgtgtc ggcgcggttc
     3361 ttggtcacaa acgtgacgat tgaaccatcg gcacgcttcg cggtcacagt gatgtcggtg
     3421 tgcggcttca ttgcattgtt gtagccttcg agattaaaaa cttcagtacc gtcgagtccc
     3481 aacgtttgtg cattttctcc gttcttaaag tggaggggta aaatccccat gccgataagg
     3541 t
//
LOCUS       DAOMPD010000278         3527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10894_length_3527_cov_2.266417, whole genome shotgun sequence.
ACCESSION   DAOMPD010000278 DAOMPD010000000
VERSION     DAOMPD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_3527_cov_2.266417"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLY93_09710"
     CDS             <1..265
                     /locus_tag="PLY93_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69792.1"
                     /translation="KRLRAWGKLGWKVLGDIPALCIRRLADETPFSVLGVLEGECITH
                     IDGETVNQPTRNFGIWLTLSSRKTLDIDTLRLGRKMSYHLTKK"
     gene            277..777
                     /locus_tag="PLY93_09715"
     CDS             277..777
                     /locus_tag="PLY93_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRP69793.1"
                     /translation="MPFRQNFYFRLALTVIIPLCVAWSFTKIVQSSFFDIARVTNESM
                     LPFLKQGDLLVIDKAAPCLKIPFTRQKFFCRSCEIGHAYVFPNPLDARRFLVKFAVAK
                     AHQTSDIIWFTQQSINKAASFDAKTSCFFEGSNTEHSIDSRHFGPIQLQKIVGKVVYP
                     PIAYNP"
     gene            774..>999
                     /locus_tag="PLY93_09720"
     CDS             774..>999
                     /locus_tag="PLY93_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69794.1"
                     /translation="MIDAKQNRSGKPFTLRYRLFLFFLFILFFILAYRAVELVVASPL
                     PPSVMGEAQKVRRGVIFDARGHELAISRDTV"
     assembly_gap    1000..1009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1010..>2006
                     /locus_tag="PLY93_09725"
     CDS             <1010..>2006
                     /locus_tag="PLY93_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP69795.1"
                     /translation="GIKPLEARLQLESVDVLSRVTGVPKAEIIQKITTHDKFFYLRRK
                     MPLEEAAPLRKLDLAGLVLQTEPDRYYPNGHLASQIIGFTGVDGEGLAGIEFGQNSVL
                     THAPSKSSATGITDADFIGQNIHLTLNSFVQHQLEKTLAKGLEESVSKHAMGVILETP
                     TGKILAMSSQPDFNPNFPNKAAEGSRKNLPISFQYEPGSTFKIFTMAALLRENMLDDK
                     HEYFCPGYFEYKGTRIACTHKHGKETFTDVMKDSCNYGVITASWQMPVLKLYENLKAF
                     GIGSYCEVALPGEAKGHLPSPKDWDHFLKMSIPIGHGVSVTPLQLALAANSIGNDG"
     assembly_gap    2007..2016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2017..2667
                     /locus_tag="PLY93_09730"
     CDS             <2017..2667
                     /locus_tag="PLY93_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP69796.1"
                     /translation="NFMPPLIIDRITDAQGNIMERFTPQVKYRVSTIENSRKIRSTLR
                     EVVKSGTGAGADLGLKEFDVCGKTGTSIKSDRKGYTDKYQASFVGFFPCDKPEITIML
                     MFDEPRGEYHQGATVAAPVFRRILREIIPTIHVGELRQVRALSTAQYRSIANEKAQMP
                     DFRGFSKKELLFQVLSKFPGDHKIQGAGYVVTQTPAAGQVINPPYSFSFTLGFPDE"
     gene            2737..>3527
                     /locus_tag="PLY93_09735"
     CDS             2737..>3527
                     /locus_tag="PLY93_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69797.1"
                     /translation="MIYTHKNWLSSVFILAIIFGSAFLMAETTTNDVAGKESPVPKSP
                     QAETEKQLEKLATENEEETTVSDKKAVKTDVKNKKSSASAQKTKNAKKDTPTGTFRPD
                     FRDQEIQDILKIFSKIIGKNIIADEKVKGKITVISPYKIPRSLAYPYLYSMLAIKGFG
                     IVEENDNLIRVVALKDALAGSVLIYLGRDEIKEELHKSDLPVTQILPIYGSKPSRLAA
                     ILKRLTSTNTELIDYDDINMLIISGSLFEVNRLIRIANLIDFAPE"
BASE COUNT          975 a          883 c          757 g          892 t
ORIGIN      
        1 caaacgcttg cgcgcgtggg ggaagcttgg ttggaaagtg ttgggcgaca tccccgcgct
       61 ttgcatccgc cgcttggcgg atgaaacgcc gttttcagtc ttgggtgtgc ttgaaggaga
      121 gtgtatcacc cacattgacg gcgaaacggt caatcaaccg acgcgcaatt tcggtatttg
      181 gcttacgctt tcaagccgca agacgctcga tatcgacacg ttgcgcttag gaagaaagat
      241 gagctaccac ttgacaaaaa aataatttct atccgcatgc cgtttcgaca aaatttttat
      301 tttcgattag cgctcacagt tatcatccct ctctgcgtgg catggtcttt tacaaaaata
      361 gtgcagtctt ctttctttga tattgcacgc gtcaccaatg agtcgatgtt acccttctta
      421 aaacagggcg atttactcgt tatcgataaa gcggcgccgt gcttaaaaat tcctttcacc
      481 cgacaaaaat ttttctgtcg ttcttgcgaa atcggtcatg cgtacgtgtt tccaaaccca
      541 ctcgacgcaa ggcgattcct cgttaaattt gccgtcgcga aggcgcatca aacaagcgac
      601 ataatctggt ttacgcaaca atccataaat aaagctgctt ctttcgatgc caagacgtct
      661 tgtttttttg aaggctcgaa taccgaacat tcgattgact cacgccattt cggaccaatt
      721 caactgcaaa agattgtcgg taaagtggtc tatcccccta ttgcatataa cccatgattg
      781 atgccaaaca aaaccgttcc ggaaaacctt tcactttgcg ataccgtctc tttttatttt
      841 ttctctttat tctttttttt attttggcat accgcgccgt tgaattggtc gttgcctcgc
      901 cgctgcctcc gtcggtgatg ggtgaagcgc agaaagtgcg gcgtggagtt attttcgacg
      961 cgcgcggaca cgagctcgcc atttcacgtg acacggtctn nnnnnnnnng gggattaaac
     1021 cgctcgaagc gcggctgcaa ctcgaaagcg tcgacgtgct ttctcgagtg acgggcgtcc
     1081 ctaaggccga gattattcaa aaaatcacaa cgcacgataa atttttttat ctccgccgta
     1141 aaatgccgct ggaggaagcc gcgcctttaa gaaaactcga cttagcgggt ttggtactcc
     1201 aaaccgaacc cgaccgttat tacccaaacg ggcacttagc gtcgcaaatc atcggcttta
     1261 ccggcgtcga cggcgaggga ctggcaggta tcgagtttgg gcaaaattca gtactcaccc
     1321 atgcgccttc aaaatcgagt gcaacgggta tcacagacgc cgatttcatt ggtcaaaaca
     1381 tacacctcac gctaaactca ttcgtgcagc accaactcga aaagaccctc gccaaaggtc
     1441 tcgaagaaag tgtctcgaaa cacgccatgg gcgtcatttt agaaacaccc accggaaaaa
     1501 ttttagcgat gtcgtctcaa cccgatttta accccaactt ccctaacaaa gccgccgaag
     1561 gctcgcgcaa aaatttaccg attagctttc aatacgaacc tgggtcgacc ttcaaaattt
     1621 ttacgatggc cgcactttta cgcgagaata tgctcgacga taaacacgaa tacttttgtc
     1681 ccggttattt tgagtacaaa ggcactcgca ttgcttgcac gcataaacac ggcaaagaga
     1741 ctttcaccga cgtgatgaaa gattcgtgta actatggcgt catcaccgct tcgtggcaaa
     1801 tgcccgtttt aaaactctat gaaaatttga aagcgtttgg catcggttcc tattgtgaag
     1861 tggcgctccc tggcgaagca aaggggcact tgcctagccc caaagactgg gatcattttt
     1921 tgaaaatgtc tatccctatc ggtcatgggg tgagcgtaac gccattgcaa ttagcgttgg
     1981 cggcaaattc aatcggcaac gacggtnnnn nnnnnnaatt ttatgcctcc cctcattatc
     2041 gaccgtatca ccgacgcgca gggcaacatc atggaacgct tcaccccgca agtaaaatac
     2101 cgtgtctcca cgattgaaaa ctcgcgtaaa attcgttcaa cgctgcgcga agtagtaaaa
     2161 agtggaaccg gcgcgggggc ggatcttggc cttaaagaat tcgacgtttg cggcaaaacg
     2221 ggtacgtcga ttaagtcaga tcgaaaagga tacaccgata aataccaagc gtctttcgta
     2281 ggtttttttc cttgcgacaa acctgaaatt acgattatgc tcatgtttga cgagccacgt
     2341 ggcgaatacc accagggggc caccgtcgcc gcgcctgttt ttcgccgtat tttgagggag
     2401 attataccca ctatccacgt cggagaattg cgccaagtgc gcgcgctttc cactgcgcaa
     2461 taccgctcga ttgcaaacga gaaagcacaa atgcccgatt tccgtggttt tagtaaaaaa
     2521 gaactccttt ttcaagtgct ttctaaattc cccggcgacc ataaaattca gggtgcgggg
     2581 tatgtggtaa cccaaacgcc tgctgctggc caagtaataa atcctccgta tagctttagt
     2641 tttactttag gatttcccga tgaataaata gaggcttgaa gcgttgtcgc acaatcgctc
     2701 tatgacagta taaaaaacca tctagtgttt actgtaatga tttatacaca taaaaattgg
     2761 ctatcgagcg tatttattct tgccattatt tttggcagcg cctttttgat ggccgaaaca
     2821 accacaaacg atgtagcagg gaaagaatcc cccgttccca aatcccccca agctgaaact
     2881 gaaaaacaat tagaaaaact ggcgacggaa aacgaagaag aaaccacagt atcggataaa
     2941 aaagcagtca agacagatgt aaaaaataaa aaaagtagcg cctctgccca aaagactaaa
     3001 aacgccaaaa aggatacccc caccggtaca ttccgccccg actttcgaga tcaagaaatt
     3061 caggacatct taaaaatctt ttcaaaaatt atcggtaaaa atattatcgc cgacgagaaa
     3121 gtaaaaggta aaattactgt catttctccg tataaaattc ctcggtcgct tgcatatccc
     3181 tacctctact ccatgcttgc gattaaaggc tttggtatcg ttgaagaaaa tgacaacctc
     3241 atccgcgtcg ttgcgctcaa agacgcgctc gccggtagtg tgcttattta tctaggccgc
     3301 gatgaaataa aagaagaatt gcataaaagc gatttgcctg tcactcaaat cctacctatt
     3361 tacggtagca agccctcgcg cttggcggca attctgaaac gcctcaccag caccaacacc
     3421 gagctcatcg attacgatga catcaacatg ttgatcatta gcggcagcct ttttgaagtc
     3481 aatcgcctca tccgcatcgc aaacctaatc gactttgccc ctgagaa
//
LOCUS       DAOMPD010000279         3506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10972_length_3506_cov_2.665604, whole genome shotgun sequence.
ACCESSION   DAOMPD010000279 DAOMPD010000000
VERSION     DAOMPD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10972_length_3506_cov_2.665604"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLY93_09740"
     CDS             complement(<1..532)
                     /locus_tag="PLY93_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP69798.1"
                     /translation="MMIRLVFASLSNRRVVVLLTSVSVALAVFLFTSVERLRVGARQS
                     FTQTISGTHIIVGARTGQIPLLLSSLFHIGAPTQNISYRTFLKYKRHAAVSWAVPLSM
                     GDSYRGFRVVGTTPDIFRYYRYGNRRPIELKTGALTLTRFKAVLGSSAARTLALRVGD
                     KIILTHGISDAQGINDH"
     gene            complement(529..1236)
                     /locus_tag="PLY93_09745"
     CDS             complement(529..1236)
                     /locus_tag="PLY93_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP69799.1"
                     /translation="MKKNNSLALSIQNLTFNYPQKQVLNIPRLEIPMGEHTLISGESG
                     CGKSTLLNLVSGILTGHNGTLQVLGRDYAGMKAAEYDRFRAENIAVVFQQFNLISYLS
                     ARENILLGVKLARKEISPLDVDALLRHLQVDAVAETRAGNLSHGQQQRIAVARALAQK
                     APLILADEPTSALDEKNARLFWDLLFNEARANNTTIVVVSHDVRHQKRFDRVIDFTKI
                     NDAVEVRKKNMPQKRSK"
     gene            complement(1243..1671)
                     /locus_tag="PLY93_09750"
     CDS             complement(1243..1671)
                     /locus_tag="PLY93_09750"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HRP69800.1"
                     /translation="MFIFLAVGFTPVLQSESAVNDISWKTLKGYNLKSKKMTTAVSRL
                     NGQMVRIPGFMVPLDDMGYATAGRFLLVPDPQSCIHAPAPPANQMIYVVMENNKQAPV
                     RYGIPVLLTGKLTIKSIRSQFGVVTFQLLGKTVEQFRGRR"
     gene            complement(1710..2516)
                     /locus_tag="PLY93_09755"
     CDS             complement(1710..2516)
                     /locus_tag="PLY93_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69801.1"
                     /translation="MAIDAATKSLYNERIGAHKAQISEHEKEIAAYKKAMTANKKLKP
                     FFHLGNVAQLLDIIRLQIEMNEVSEQMMQVKNNSFLDNARKNLYKIFADLDVVVKGDI
                     DEPIDFNRTELDKITPFTPKQRLNLYRHLRRSIDKLIESYGTNTKWLWSFPELYAKSA
                     NLGKNLIDFREVQSKRDPRETYFYDRKEMVQKIIDDLMDASNRYRDKYEISTKATGDL
                     IMAIKLLDALRRVAVVVKDDAMAAKAKTGIDSYKARIEAEKEEKKAPVRK"
     gene            complement(2558..2719)
                     /locus_tag="PLY93_09760"
     CDS             complement(2558..2719)
                     /locus_tag="PLY93_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69802.1"
                     /translation="MRVEETSNNTARHIYEAQQVATQEAQNRQEPVQKDAPAQATGTA
                     ATSGIDTYA"
     gene            complement(2759..>3506)
                     /locus_tag="PLY93_09765"
     CDS             complement(2759..>3506)
                     /locus_tag="PLY93_09765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRP69803.1"
                     /translation="RMLFGALSLIFPDGERFFIRSVKYFADQIKDESLIADVKNFIGQ
                     EAQHTKLHEVFNKNVLGPEYDTTPFLTWYRDVVYGKIEEEATKLFGHKLSLSITAAAE
                     HFTATWAAHGLSSGYLEEHVKNQELLDFMQWHAVDEIEHKHVAFDVLQEVDDSYLLRV
                     AGMLFCATLLPFVVTVAFASLVKQSHKLDIFTFFRDGVYEGAKGGMARSFIPAIIEYF
                     KPGFHPSDNDNYAEAAKVAERIEKRMQYVA"
BASE COUNT          853 a          830 c          846 g          977 t
ORIGIN      
        1 cgtggtcgtt tatcccttgc gcgtcgctga tgccgtgcgt cagtataatt ttatcgccca
       61 cacgcaaagc gagtgtgcgc gccgccgacg aacccaaaac agctttgaag cgggttaaag
      121 ttaatgcacc cgttttaagc tctatgggcc gccgattacc gtaacggtaa tagcgaaaaa
      181 tatcgggtgt agtgcccacg acccgaaagc cacggtaact atcgcccatc gaaagtggca
      241 ccgcccagga aacagccgcg tgccttttgt atttcaaaaa tgtgcggtat gaaatatttt
      301 gtgtcggtgc gccaatgtga aaaagcgacg acaacaagag cggtatttga cctgtgcgtg
      361 cccccacaat gatgtgcgtg ccgcttatcg tctgtgtgaa actttggcgt gcccccactc
      421 gtaaacgctc aacgctggta aaaagaaaaa cagccagcgc caccgaaacg cttgtaagga
      481 gtacaacgac gcgtcggttc gatagcgaag cgaacaccaa gcggatcatc atttgctcct
      541 tttttgcggc atattttttt tccgtacttc gacggcgtcg ttaatttttg taaaatcgat
      601 aacgcgatca aagcgttttt ggtgtcgcac atcgtgcgag acgaccacaa tcgtggtatt
      661 gttagcgcgc gcctcgttaa aaagaagatc ccaaaaaagg cgcgcattct tttcatcgag
      721 tgcgctggtg ggctcgtcgg ctaaaatgag cggggctttt tgcgccaacg cacgcgccac
      781 cgcaatacgc tgttgttgac cgtggcttaa attgcccgcg cgcgtctctg caacggcgtc
      841 gacttgcaag tgtcgcagca atgcgtcaac gtcgagcgga gatatttctt tgcgcgcaag
      901 cttaacgcct aaaagaatat tctcgcgcgc gctgaggtat gaaattaaat tgaactgttg
      961 gaaaacgacg gcgatattct cggcgcggaa tctgtcgtat tcggcggctt tcataccggc
     1021 ataatctctc cccaaaactt ggagcgtgcc gttgtggccg gttaaaattc cagacaccaa
     1081 atttaggaga gtgcttttgc cacagccgct ttctcccgaa atgagcgtgt gctcacccat
     1141 cggaatttca aggcggggta tattgagcac ttgcttttgc ggatagttga acgttaaatt
     1201 ttgaatagaa agcgcaaggg aattattttt tttcattctt aattatctac gccctcgaaa
     1261 ctgctctacg gtcttaccca acaattgaaa cgtaacgact ccaaattgcg accggatcga
     1321 ttttattgta agtttgccgg tgagtaaaac gggtatacca taacgcactg gggcttgttt
     1381 attgttctcc atcacgacat aaatcatttg gtttgcaggc ggtgccggtg catggatgca
     1441 cgattgtgga tcgggcacca ataaaaaacg ccccgcagtc gcatagccca tgtcgtctaa
     1501 cggcaccata aacccgggta tgcggaccat ttgtccgttc aagcgggaaa cggcggttgt
     1561 catttttttg ctcttgaggt tatagccttt gagtgtcttc cacgaaatat cgtttaccgc
     1621 gctttcgctt tgcagaaccg gtgtaaatcc cactgcaaga aaaataaaca cagcccggca
     1681 aaaatttttt ctgaaaaggc ccacgggtat tattttctta ccggcgcctt tttttcttct
     1741 ttttcggctt cgatacgcgc tttataagag tcgatacccg tttttgcttt ggctgccatc
     1801 gcgtcgtctt tcacaacaac cgccacacgg cgtagcgcgt ctaaaagttt gatggccata
     1861 atcaaatcac ccgtggcttt tgtggagatt tcatatttgt cgcggtaacg gtttgaggcg
     1921 tccatgaggt cgtcgataat tttttgtacc atttctttcc ggtcgtaaaa gtaagtttcg
     1981 cgcgggtcgc gcttcgattg cacctcgcga aaatcgatga gattcttgcc taagtttgcg
     2041 cttttagcat aaagctcggg gaaagaccaa agccattttg tattagtgcc ataagactca
     2101 ataagtttat cgatggagcg gcgtaaatgg cggtacaaat tgagtcgctg tttgggcgta
     2161 aacggagtaa ttttatcgag ttcggtacgg ttgaaatcga ttggttcgtc gatgtcgcct
     2221 ttaacgacca cgtctaaatc ggcaaaaatt ttatatagat ttttacgcgc attatccaag
     2281 aacgagttat ttttaacctg catcatctgt tctgaaactt cattcatttc aatttgcagc
     2341 cgaatgatgt cgagtaattg ggcgacgtta cccaagtgaa aaaaaggttt taatttttta
     2401 ttcgccgtca tcgctttttt gtaagcggca atttcttttt cgtgctcgga tatttgcgct
     2461 ttatgcgcgc caatgcgttc gttgtaaagc gatttggtcg ccgcgtcgat tgccatagtt
     2521 aatcttcggc ctttggaggc gtataattaa aggaagatta agcgtaagta tcgatgcccg
     2581 acgtggcggc ggtacccgtc gcctgggcag gcgcgtcctt ttgcaccggt tcttgccgat
     2641 tctgtgcttc ttgcgtggct acctgctgtg cttcgtagat atgccgcgcg gtattgttgc
     2701 tggtttcttc tactcgcata ctactatatt agcgaataaa acgcacaaag gccaattttt
     2761 atgccacgta ttgcatgcgt ttttcaatac gctcggcaac ctttgccgcc tctgcataat
     2821 tgtcgttatc cgacgggtgg aatccgggtt taaaatactc gatgatggca ggtataaaag
     2881 agcgcgccat gccgcctttt gccccttcgt aaacgccatc tctaaagaaa gtaaagatat
     2941 ctaatttgtg cgattgcttc acgagcgatg caaatgccac cgtgacaaca aaaggtaaca
     3001 gcgttgcgca aaagagcata cctgccacgc gcaaaaggta tgaatcatcg acttcttgta
     3061 aaacgtcgaa agcaacgtgc ttgtgctcaa tttcgtccac cgcgtgccac tgcatgaaat
     3121 cgagcagttc ttggttttta acgtgttctt ctaaataccc tgaggagaga ccgtgcgccg
     3181 cccacgttgc ggtgaaatgt tcggcggcgg cggtaatcga aagcgaaagt ttgtgtccaa
     3241 agagcttcgt cgcctcttct tcaattttgc cgtacaccac gtcgcggtac cacgttaaaa
     3301 agggcgtcgt atcgtactct gggcccaaca cgtttttatt aaacacctcg tgtaactttg
     3361 tgtgctgcgc ttcttgcccg ataaaatttt tgacatcggc gatgagcgat tcgtctttaa
     3421 tttgatcggc aaagtattta acgctgcgga taaaaaagcg ttcaccgtcg ggaaaaatta
     3481 gactgagagc gccaaaaagc atacgg
//
LOCUS       DAOMPD010000280         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_10992_length_3502_cov_2.828547, whole genome shotgun sequence.
ACCESSION   DAOMPD010000280 DAOMPD010000000
VERSION     DAOMPD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10992_length_3502_cov_2.828547"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..712
                     /locus_tag="PLY93_09770"
     CDS             479..712
                     /locus_tag="PLY93_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRP69804.1"
                     /translation="MDLANLMRPRKICLCKSVSRQDIADAVQGGANTFRKLVEKTGAT
                     TGCGTCYHEVYAVFCEERDKLKAEQSDQSMLEF"
     gene            702..2651
                     /locus_tag="PLY93_09775"
     CDS             702..2651
                     /locus_tag="PLY93_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69805.1"
                     /translation="MSFKLSLLQLLPIKERGFLSMLKKITLVSVLIPLLTLSIAAKEI
                     QENPLAKKLERYQPYDLSEIEASAQSLNFNPRLVAEELGEKLNQFYLSDPSAKKIVNR
                     QDNDSNTAWYRKEWNVNIQRLFMRDLLAKPQKSFLIKEAPNIGMLHYYYAEAQMKLKK
                     PYHAAFHFAQALRYRSLKLDDEIYVDKDRLTLVKAGNAEVADADNYRQVKATLAEKTK
                     EKERLEEELILLDDRSRSPHTPAPQAGTSVEAERQRQNRLIAEQKARTEAALARVKGE
                     FDKAKTDFEGAKAKFEEHAKAYNRKSADMLIEMSALVRQIEDAIKERSKVLNKKALYK
                     TNFNQTLQHDYSQNRQFAAYANILEAATRLDAENPKTNFALAEEYRTSQDISRSIDAY
                     ERALDANEKPRVGFELKNEEVQKAHASLGALYYAKHRYVDAAYFYEKAYNATAESREK
                     QNLGWQLAKLHAEKTGNYEKAISLFENYLQLISALNPTDITDRTQWIKERFLANRYLM
                     QMFDKKNVPVKVKAALQNAEAAHQDLEKLIVDLRERNNKTYQDLQTAKKSLLDDSRSR
                     ELNVYLKLESDFEKQKHELSIIETVRNSLPLAQFYRQYATFLEAQHDIQGAIDVYAMA
                     EKHGIGADAARREQNRLKTLLGGGV"
     gene            complement(2670..3374)
                     /locus_tag="PLY93_09780"
     CDS             complement(2670..3374)
                     /locus_tag="PLY93_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69806.1"
                     /translation="MQKIRITAALLIAVFIAGACKGDKSPWIWKIEGKTVTLNQLEEA
                     YSGFGVLMKDQLQQRMGRFLPEEEFKQLLEDPDRTGNPQLAELFKSLGKDLFADQYQD
                     MLILNAEAQKDGFTKRADVKAKLEFLQKYFLANMYLFDQLKLQDIKINDAEALEEWQK
                     AKKADKRLATVPLDQGLEMVKGQMVMKVAMEKQRTLMKNAKQKYRIDTNKDFDIAEYV
                     KKTREEARKKRETGTK"
BASE COUNT         1023 a          855 c          733 g          891 t
ORIGIN      
        1 gcaaaaacag caacagcaca atcacggcca acacaacgag gagtatattt ggtttgttca
       61 tttgcttttt tttcaatttg agcgctcccc gtcaagctct tagtaattcc aacctatggc
      121 aagcatacaa ccaaacgctg tcagctatgt tgagcttgca catgtaaaag aaagtttttc
      181 cttaaatttt acctgatttt gaccactcca gtgctcgtgt agcatagtta ccccctaatg
      241 tgacataatc ccgaaaatcg gtatagttat tccttaatgt gacataatcc gaaaaatcga
      301 catagttacc ccctgatgtg acataatcta aaaaatagac atgttacctt gacagcttgc
      361 gtcctccaac ttactataag gctctgccag tatggtacgc gactccttgg ttggatacgt
      421 tccgacccat ttattatatt tattgccgcc tcatataaaa gataataatg tctaaataat
      481 ggacttggcg aacctcatgc gccctcggaa aatatgcctg tgcaaaagcg tttcacggca
      541 ggatatcgcc gatgccgtcc aaggcggtgc gaacaccttc cgcaagttag ttgagaaaac
      601 aggcgctaca accggttgcg gcacttgtta ccatgaagtg tacgccgtat tttgcgaaga
      661 aagggataag ctcaaggcgg agcaaagcga ccaatcgatg cttgagtttt aagctttctt
      721 tattacaact tttaccgata aaagaaaggg gatttctatc gatgcttaaa aaaatcaccc
      781 tcgtctcagt cttaatccca cttttgacgc tttctattgc cgcaaaagaa attcaagaaa
      841 acccactcgc aaaaaagctc gaacgctacc aaccgtacga tctcagtgaa attgaggcgt
      901 ccgctcagtc gctcaacttt aaccctcgtc ttgtggccga agaactgggt gaaaaactca
      961 accagtttta tttgagcgac ccttctgcca aaaaaattgt caaccgccaa gataatgatt
     1021 ccaacaccgc gtggtaccgc aaagaatgga atgtgaatat ccaacgactt tttatgcggg
     1081 atttactcgc caaaccacaa aagtcatttt taattaaaga agctccaaac attggtatgc
     1141 tgcattatta ttatgcggaa gcgcaaatga agcttaaaaa accataccat gcggcgtttc
     1201 attttgcgca ggcgttgcgt tatcgtagtc tcaagctcga cgatgaaatt tatgtcgaca
     1261 aagaccgttt aacgctcgtg aaagcgggaa acgccgaggt ggccgatgcg gacaactacc
     1321 gtcaagtcaa agcgacactc gccgaaaaaa ccaaagagaa agaacgttta gaagaagagt
     1381 tgatactctt ggacgaccgc tcgcgttcac cgcacacacc cgcgccccaa gctggaacga
     1441 gtgtcgaagc cgaacgccaa cgccaaaacc gtctcatcgc cgaacaaaaa gcgcgcaccg
     1501 aggctgcgct cgcgcgtgta aaaggcgagt tcgacaaagc caaaaccgac tttgaagggg
     1561 caaaagcaaa atttgaagaa cacgccaaag cgtataaccg taaatcagcg gacatgctca
     1621 ttgagatgtc agcattggtt cgccaaattg aagacgctat caaagaacgc tcgaaagtcc
     1681 tcaacaagaa agcgctctat aaaacaaact tcaaccaaac gctgcaacac gactacagtc
     1741 aaaaccgtca attcgccgcg tacgccaata tcttagaggc agccacgcgt cttgacgctg
     1801 aaaatcctaa aacgaatttt gctctcgccg aggaataccg cacctcgcaa gacatctcgc
     1861 gcagcatcga cgcgtacgaa cgcgcgctcg atgcgaacga aaaaccacgc gtgggctttg
     1921 agctcaaaaa tgaagaagtg caaaaggcgc acgcatcgtt gggcgcgctc tactacgcca
     1981 agcaccgcta cgtcgacgcg gcgtactttt acgaaaaagc atacaatgca accgctgaaa
     2041 gccgcgagaa gcaaaacttg ggttggcagt tggctaaact acacgcggaa aaaaccggca
     2101 attacgaaaa agccatttcc ctttttgaaa attatttgca gttgattagc gcgctcaacc
     2161 ccaccgacat caccgaccgc acgcagtgga tcaaagaacg ttttttagcc aaccgctatc
     2221 tcatgcaaat gttcgataaa aaaaatgtgc ccgtaaaagt gaaagccgca ttacaaaacg
     2281 ccgaggctgc gcaccaagac ttggaaaaac tcatcgtcga cttacgcgaa cgcaacaaca
     2341 agacttacca agatttacag accgccaaaa aaagtctttt ggacgattca cgcagtcgcg
     2401 agctcaacgt ctacttgaaa ctggagtccg actttgaaaa acagaagcat gaactctcta
     2461 tcattgaaac ggtgcgcaac agtttgccgc tcgcgcagtt ttaccgtcag tacgccacct
     2521 ttctcgaagc gcagcacgat attcaaggcg ccatcgacgt gtacgccatg gcagagaaac
     2581 acggcatcgg cgctgatgcc gcccgccgcg aacaaaatcg actgaaaact ttactcggcg
     2641 ggggcgtcta aaaagggcgg atttaacgct tattttgttc cggtttcgcg ctttttacgc
     2701 gcttcctctc gggttttttt cacgtattct gcaatatcga agtctttgtt agtgtcgatg
     2761 cggtattttt gtttcgcatt tttcataagc gtacgttgtt tttccatagc gactttcatc
     2821 accatttggc cttttaccat ttcaagccct tggtctaaag gtacggtggc caaacgttta
     2881 tcggcttttt tcgctttttg ccattcttcg agcgcttcgg cgtcgttaat tttgatatct
     2941 tgtaatttta gttgatcaaa aagatacatg tttgcaagaa agtatttttg gaggaattcc
     3001 agttttgctt tcacatcggc gcgtttagta aatccgtctt tctgcgcctc ggcatttaag
     3061 atgagcatat cttggtattg gtcggcaaat aaatctttac ccaaactttt gaaaagttcg
     3121 gcaagctgtg gattccctgt acgatcggga tcttctaaga gctgcttaaa ttcctcttcg
     3181 ggtaaaaaac gacccatccg ttgttgtaat tgatccttca tcaaaacgcc aaaaccactg
     3241 tatgcttctt cgagttgatt taacgttact gtttttcctt caattttcca tatccaagga
     3301 gatttatcgc ctttacacgc acccgcgata aagaccgcaa tgagaagtgc ggcggtaatt
     3361 ctaattttct gcatatattg ctccataata aatagtgtgg cagtttttgc gttttgcaat
     3421 aagttgcaaa gcacaaactt tttagtaagt atgcaaccca cagagataac tgacactttt
     3481 ggggtcaagt tgagtgcgtt tt
//
LOCUS       DAOMPD010000281         3492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11023_length_3492_cov_3.059936, whole genome shotgun sequence.
ACCESSION   DAOMPD010000281 DAOMPD010000000
VERSION     DAOMPD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11023_length_3492_cov_3.059936"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLY93_09785"
     CDS             complement(<1..684)
                     /locus_tag="PLY93_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69807.1"
                     /translation="MQKSNRGYVVYPNEFSRRREKLELPNQVLYTPQQYKKLDDALEG
                     GALITGEIYEYEKKTNTEKTLNNNDDTPIDIKSVLVKLHHYNARTKKRTTISVYAKDE
                     NIVFEMLRAIFPLENDRYLLNPIQAGASIALVTPLDDAEANDFYMRLLNDKFQVRLLS
                     GNDFIKGNPTILESLRNISSRFISYETAREVKENPPFVISAATDIQAEAQEHEEYLTT
                     ENEYLKNFSS"
     gene            complement(863..1456)
                     /locus_tag="PLY93_09790"
     CDS             complement(863..1456)
                     /locus_tag="PLY93_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69808.1"
                     /translation="MNIFIKNRCYSLLLFLLLALGCKSTQNQYVIKEAIPTNTNIAIL
                     VEGENDFKNAVFMEFMNAGYSVKAFNATDFYTTDDVLDVRSLKKTAFVQNIFDTKNSQ
                     RQVAVADKVFENIYKLHIFNFENLKVEMLQALRAKLNARYVILLSLKNWDQGYSWTRV
                     IRLDNMDLVFVQNYKANRKDDLKSVVDQMIQTMKKGK"
     gene            1553..1903
                     /locus_tag="PLY93_09795"
     CDS             1553..1903
                     /locus_tag="PLY93_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69809.1"
                     /translation="MALQKKYRLWLQTVGVALLVSFLTLLFFQQRKKSPDRVALGEEV
                     YASHDCTDCHIDTEKLREKKNKKEVGLIRVRFEKTVLTQFLSTDPRHTSYTLMTDEDR
                     ANLIEYLTSLVVKK"
     gene            2146..2691
                     /locus_tag="PLY93_09800"
     CDS             2146..2691
                     /locus_tag="PLY93_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69810.1"
                     /translation="MLPLHAQVETETWRFKPQIGLWFGPVTPVPGTAIADVLITSLGG
                     GIFFRGNLPTNTLRTEIGVSYGYYTSRSTARLHSLPVYGALVYTLPINLPLSFFVKGG
                     LGSNYLKNAIEGSQNWLPTGYLGFETSFPAGKLVNIGVRADYYYVYEKHLSAPANNPN
                     YEVHNGHFLNFGLMVNFNLSR"
     gene            2694..>3492
                     /locus_tag="PLY93_09805"
     CDS             2694..>3492
                     /locus_tag="PLY93_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69811.1"
                     /translation="MKKVGFTLLLSCWAVFTHCTDQLPLSDLVNNSITLKFLGTYESN
                     DPYGDLTLYKDDIINSGITGNAAWTNSTDIYTYANGLNISRIKYYIDIAEIRIAQGQG
                     KSTSQDIDDYWSQFAIKRELLCSDYSVAGGGRSLPNCSSNSGIQKLANFFNGGFTYPA
                     SDVSPGSYNHLGIYFRRFNTYPAARFNAAGQFMNASGSATTQDASEQSMTAAFDNNPV
                     YGFDAENLLLNPYGATTSEPLMFPLQRKDLSIQVPGGDEPYVLEVRVF"
BASE COUNT          918 a          774 c          775 g         1025 t
ORIGIN      
        1 ggaagaaaag ttttttaagt actcgttctc cgtcgttaag tactcctcgt gctcttgtgc
       61 ttccgcctga atatctgtgg cagcggaaat tacaaacgga ggattctctt taacttcacg
      121 cgccgtttcg taggaaataa atcgactgct aatgttgcgc agcgactcaa gtattgttgg
      181 attgcctttt ataaaatcat ttccagagag caagcgcact tggaacttgt cgttcaacaa
      241 ccgcatataa aagtcattcg cttcggcatc gtcgagcggt gtgacaaggg caatcgatgc
      301 gcctgcttga attggattta gtaaataacg gtcgttctcg agcggaaaaa tcgctcgtag
      361 catttcaaac acaatatttt cgtctttggc atacaccgaa attgtcgtgc gcttctttgt
      421 gcgcgcgttg tagtggtgga gcttcactaa caccgatttt atatcgatgg gggtgtcgtc
      481 gttattgttg agtgtttttt ctgtatttgt ttttttctcg tattcgtata tttcaccggt
      541 gatgagtgcg ccgccttcta aagcgtcgtc taattttttg tactgttgag gggtataaag
      601 cacttggttt ggcaactcga gtttctcgcg ccgacgggaa aactcgttgg ggtacacgac
      661 atagccacga tttgattttt gcaacgcttt actgagcacg atcgggtagc gtaaccttat
      721 ttcatgagaa atcggtgtct cggtacggct tgcaaaaagg ccgatcactg tcggcgtatt
      781 agcagcaagt ggtgataaaa cgcccgcccc cagtaagcta aaaagcagag cggaaaaaat
      841 gctctgtcga aacacgttgt ctctacttgc cttttttcat tgtttgaatc atttggtcga
      901 caacgctttt aagatcgtct tttctgttcg ctttatagtt ttgcacaaaa actaagtcca
      961 tattgtcgag acgaataacg cgtgtccacg aatagccttg atcccagttt ttgagagaaa
     1021 gcagaataac gtaacgcgca ttaagtttag cgcgtaatgc ttgcagcatt tcaactttca
     1081 gattttcaaa attgaaaatg tgtagtttgt aaatattttc aaacacttta tcggccacgg
     1141 caacttgacg ttgcgaattt tttgtgtcga aaatattttg tacaaatgct gtttttttga
     1201 gcgaacgtac gtcgagcacg tcgtcggttg tgtagaaatc ggtcgcgttg aatgccttca
     1261 ctgagtagcc agcgttcata aattccataa ataccgcgtt cttaaaatcg ttttctcctt
     1321 cgacaagaat agcgatgttc gtattcgtag ggattgcttc tttaattaca tactgattct
     1381 gtgtggattt acaaccaagc gccaataata gaaaaagtaa aagactataa cagcgattct
     1441 taataaaaat attcatggtt actatgtggt tgagttgtct gcattgtaaa taagaattta
     1501 gggttggcac gcagactcta aacttctatt attcacacgg ttgcaagcgc gcatggctct
     1561 tcaaaaaaaa taccgtcttt ggttgcaaac tgttggagtg gcgttgctcg tctcttttct
     1621 aacgcttctt ttctttcagc aacggaaaaa atccccagac cgtgtggcat tgggagaaga
     1681 ggtctacgct tcgcacgatt gcaccgattg tcacatcgac acagaaaaat tacgcgagaa
     1741 aaaaaataaa aaagaagtgg gattgatccg cgtacgcttt gaaaaaaccg tgcttacaca
     1801 atttttatca accgacccac gacacacttc gtacacgctg atgacagacg aagaccgtgc
     1861 gaatttaatc gaatacctaa catcactcgt ggtgaagaag taaatcggtc aattttcctt
     1921 cctttataaa gaaattgtaa tttttttact cgaaatgact aatcgggata gttatccctt
     1981 aatgcgacat aacgcagtat tgggcacagg ctctcttccg tatttgctca tcggctcgtt
     2041 ggcttgacga cacgtcacgt acacgctttg agtcaataaa ttctcatgct caaaatcgct
     2101 cgttactgtt cgacgttctt tctctcattt attgttactc tcggtatgct gccactccac
     2161 gcacaagtcg agacggagac atggcgcttt aaaccacaaa tcggattgtg gtttggccct
     2221 gtgacgcccg tgccgggcac cgccattgca gatgttttaa tcaccagttt aggagggggc
     2281 attttctttc gtggtaattt acccaccaac accttgcgta cagaaattgg cgtctcttac
     2341 ggctattaca cttcgcgctc gacggcgcgc ctccattcgc taccggttta tggagcgctt
     2401 gtttatacac tgcccatcaa cctgccgctt tctttttttg tgaagggcgg cttgggtagc
     2461 aactatttga aaaacgcgat tgaaggttcg cagaactggt tgcccaccgg ttacttgggg
     2521 tttgaaacaa gtttccccgc agggaaactt gtgaacattg gcgtgcgcgc agattactac
     2581 tatgtctacg aaaaacattt aagcgcacct gcgaataatc ccaattacga agtgcacaat
     2641 ggccactttt taaattttgg tttgatggtg aattttaacc taagtaggta aatatgaaaa
     2701 aagtgggatt tacacttctt ctgagctgtt gggctgtatt cacccattgc accgaccagt
     2761 tgccgctttc cgatttagtg aacaattcaa tcacgcttaa atttttgggc acctatgaaa
     2821 gcaatgaccc ttacggcgat ttgacgctct acaaagacga tattataaat agtggcatca
     2881 ccggcaatgc ggcgtggacg aacagcaccg atatttatac ctatgcaaat gggctcaata
     2941 tcagccggat caaatactat atcgacatcg ctgaaatccg cattgcgcaa ggacaaggaa
     3001 aatctaccag ccaagacatc gacgattact ggtcgcaatt cgccattaag cgtgaactct
     3061 tatgctccga ctattcggtg gcaggtggtg gccgcagttt accgaattgt tcctcgaaca
     3121 gcggtattca aaaactcgcc aattttttta acggtgggtt tacataccct gcaagcgacg
     3181 tttcaccggg gagttacaac catttaggca tttatttccg ccgctttaac acctaccctg
     3241 cggcgcgctt taacgcagca ggccagttta tgaatgcgag tgggagcgcc acgactcaag
     3301 acgcaagcga gcaatcgatg acggctgcgt tcgataataa tcctgtttat ggcttcgacg
     3361 cagaaaactt gttattgaat ccgtatggag caaccaccag cgaaccgttg atgttcccat
     3421 tgcaacgcaa agatttaagt attcaagtgc ctgggggaga cgagccctat gtactcgaag
     3481 tgcgcgtttt ct
//
LOCUS       DAOMPD010000282         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11065_length_3481_cov_2.428196, whole genome shotgun sequence.
ACCESSION   DAOMPD010000282 DAOMPD010000000
VERSION     DAOMPD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11065_length_3481_cov_2.428196"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..>2160)
                     /locus_tag="PLY93_09810"
     CDS             complement(169..>2160)
                     /locus_tag="PLY93_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRP69812.1"
                     /translation="QDVIVREEDCGTSDGFNMSADKEGEKVIVSLKQKIVGRYLQQDI
                     KDPNTDEVIYIRNTLLTEEIATQIETMGYETIKVRSILTCESTTGVCSHCYGIDLSTL
                     QPVEKGEAVGTIAAQSIGQPGTQLTMRTFHIGGTATTKSAESTIKAPYDAVVLQIQGR
                     QVNNRQKQRILAKRGSIVIAQILARYTKSQFVEFTHEAGSKLIKGEIIGKLKDKEGHE
                     ALLYTPAAGTIFIEKNEIYLLASEYAIQLGAGSVLHAEEKDVVKASQLLCEYDPYNSL
                     IIATHSGRLELVDVEDGKNLRMEDADDRNIGKLKKIVAYKNEKLVPRGHIYDKGKLVA
                     ETPLPVGAILMVENGASVQEGDIVAKIESKAEKTRDITGGLPRIDELFEARHPKDPCH
                     LAEIDGMLVDTGTIVREKRVLKIIPDGLTGDEAEANAVSIAIPLHKQIQVHDGEMVVR
                     GEPLDDGVMDPHDLLRIMGEEIAQRYLIREIQEVYRLQGVYINDKHIEIIIRQMMRKV
                     EVIDAGDTIFVPMSQVDRALFKYENQRVERDGGAEATSTPILMGLTKASLNSESFLSA
                     ASFQETTRVLTDAAIKGKDDPLEGLKENIIIGHLIPAGTGMRDYRAVRAYKTVMGDLF
                     YTEEELEEMYNGAQEKAEEVVALAPTGRSTDDDEDSDEE"
     assembly_gap    2161..2198
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2199..3439)
                     /locus_tag="PLY93_09815"
     CDS             complement(<2199..3439)
                     /locus_tag="PLY93_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRP69813.1"
                     /translation="MALELFKPFIMKWLVDKEYTQNIKSAKRMIENEEREVFEGLEEV
                     IHEHPVLLNRAPTLHRLGIQAFLPVLVEGKAIQLHPLVCHAFNADFDGDQMAIHVPLS
                     PRSQLEAWLLMLSAHNLLNPANGQPIVGPTQDMILGLYYLTASLPGAKGEGLIYDNMD
                     ELSYAIDRGVVDYRAKVRYVQNDKIFETTPGRLIFNQLLPPNYPFVNQALTNKGVNEI
                     IADLYKVKGAAVTVKFLDAIKRLGFQSATKFAPTISIDDVKIPESKARIIEKANKDVE
                     RVETEHRKGVITNEERYKKVIDIWTNANEKITEDLFTLLSEDQAGFNPIYAMAVSGAR
                     GSRSQIRQLAGMRGLMAKPSGDIIELAIRSNFKEGLGVLEFYISTSGARKGLADTALK
                     TADAGYLTRRLVDVSPDVIVR"
BASE COUNT          787 a          860 c          804 g          992 t
ORIGIN      
        1 gcgggggatt ttgttttata gaccgctctt tcacggccaa aacgaatcaa ttggttaatt
       61 gttggcatac tttcctttta tttcactatt atgttggtac tcaaattatt cagtggcaaa
      121 tttgtttttt ttgacaccgc gtcaacgtct ctaagagttt tacaaccact attcttcatc
      181 gctatcctcg tcgtcatcgg ttgatctgcc cgtcggcgct aacgccacga cttcctcggc
      241 cttttcttgc gcaccgttgt acatttcttc cagctcttcc tcggtgtaaa acaagtctcc
      301 catcaccgtt ttgtacgcac gcaccgcgcg gtagtcgcgc atacccgtgc ctgctggaat
      361 caaatgaccg ataatgatat tctctttaag cccttcgagg ggatcgtctt tgcctttgat
      421 agcggcatcg gtaaggacgc gcgttgtttc ttggaacgac gctgccgata aaaagctttc
      481 agagttgaga gaagcttttg taagtcccat caaaatagga gtcgaggttg cttctgcacc
      541 gccgtcgcgc tcgacgcgtt ggttttcata cttgaagaga gctctgtcga cttggctcat
      601 cggtacaaaa attgtatcgc ctgcatcgat gacttcgact ttgcgcatca tttgacggat
      661 gataatctca atatgtttat cgttgatata cacgccttgc agacggtaca cttcttgaat
      721 ctcgcggatg aggtaacgct gtgcgatttc ttcgcccatg atgcgtaata aatcgtgcgg
      781 atccatcacg ccgtcgtcta aaggttcgcc gcgcacaacc atttcaccgt cgtgcacctg
      841 gatttgctta tgcaaaggaa tcgcaatcga aaccgcgttc gcttctgcct cgtcgccagt
      901 caagccatcg ggaataattt ttaggacacg cttttcacgc acaatcgtac cggtgtcgac
      961 caacataccg tcgatctccg ccaaatgaca ggggtctttg ggatggcgtg cttcaaaaag
     1021 ctcgtcgatg cgtggtagac cgccggtaat atcgcgcgtt ttttccgctt tcgattcaat
     1081 ttttgcaacg atatcgcctt cttgaacgga tgcgccattc tcgaccatga gaatcgcacc
     1141 cacgggtagc ggtgtttctg cgacaagttt acctttgtcg tagatgtgcc cacgcggcac
     1201 cagtttttcg tttttgtagg ctacaatttt tttgagttta ccaatgttgc ggtcgtcggc
     1261 gtcttccatg cgtaagtttt tgccgtcctc aacgtcgacc aactctaagc gtcccgaatg
     1321 cgtcgcgatg atgagcgagt tgtaagggtc gtattcgcaa agcaattggc ttgcttttac
     1381 aacatctttc tcttccgcgt ggagtaccga gcccgcgcct aactgaatag cgtattcaga
     1441 ggccaataag tagatttcat ttttttcaat aaaaatggtg cccgcagccg gtgtgtataa
     1501 caacgcttcg tggccttctt tatctttgag tttgccgata atctcgcctt taatgagctt
     1561 cgatcccgct tcgtgcgtaa actcgacaaa ttgtgatttt gtataccgcg ctaaaatttg
     1621 tgcaatcaca atagagccac gcttcgccag aatacgttgc ttttggcggt tgttgacttg
     1681 acggccttgg atttgcaaaa cgacggcatc gtatggagct ttaatcgtac tctcggcaga
     1741 tttcgtcgtc gcagtaccgc cgatgtggaa cgtacgcatg gtgagctggg tacctggttg
     1801 accaatcgat tgcgctgcaa tcgtacccac cgcttcccct ttttccaccg gttgcaacgt
     1861 cgacaaatcg atgccgtaac aatgcgaaca aacgccggtt gtcgattcgc acgttaaaat
     1921 cgaacgtact ttgatggtct catagcccat cgtttcaatt tgcgttgcga tctcttctgt
     1981 gagaagagta ttgcggatat agatcacttc atcggtgttt ggatctttga tgtcttgctg
     2041 taaataacga ccgacaattt tttgcttgag gctcacaatc accttttcgc cttctttgtc
     2101 ggcagacatg ttaaatccat cggaggtgcc acaatcttct tcgcggacaa tcacgtcttg
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gcggacaatc acgtctggcg
     2221 aaacatcgac caagcggcgt gttaaataac ccgcgtcggc agttttgagc gcagtgtcgg
     2281 ccaaaccttt acgggcgccc gaggtggaaa tatagaactc caacacaccc aacccttctt
     2341 taaagtttga acggatcgca agttcgataa tatcgcccga tggtttcgcc atgagcccac
     2401 gcataccggc cagctgacgg atttgcgagc gcgaaccgcg cgctcccgaa accgccatcg
     2461 cataaatggg gttaaagccc gcctggtctt cactcaaaag agtgaataaa tcttcggtaa
     2521 ttttttcgtt agcgttggtc caaatatcaa tgactttttt gtaacgctct tcgttggtaa
     2581 tcaccccttt gcggtgttca gtttctacgc gctcaacgtc tttgttggct ttttcaataa
     2641 tgcgcgcttt cgattcggga atttttacat cgtcgataga gatagtgggg gcaaacttgg
     2701 tcgccgattg aaagcctaaa cgtttaatcg catccaaaaa tttaaccgtc accgccgcgc
     2761 cttttacttt gtaaaggtcg gcaataatct cgttcacgcc cttgtttgtc aacgcttggt
     2821 ttacaaacgg ataattgggg ggtaaaagtt gattgaaaat caaacggccg ggagttgttt
     2881 caaaaatttt atcattttga acataccgca ctttggcgcg gtaatcgaca acaccgcggt
     2941 cgattgcata gctgagctcg tccatgttat cataaatgag tccctcgcct tttgcgccag
     3001 gcaaacttgc cgttaaatag tagagtccca aaatcatgtc ttgggtgggc ccaacaatcg
     3061 gctgaccatt tgcagggttg agaagattgt gcgccgaaag catgagcaac cacgcttcta
     3121 attgcgagcg tggcgaaagc ggcacatgga ttgccatctg gtcgccgtca aagtcagcgt
     3181 taaacgcgtg gcacaccaaa ggatgcaact ggattgcttt accttcgacg agaacaggta
     3241 agaacgcctg aatacccaaa cggtgcaacg tgggtgcgcg gttgagcaac acgggatgtt
     3301 cgtggatgac ttcttcgagc ccttcgaaaa cttcgcgctc ttcgttttca atcatgcgct
     3361 tggcagattt aatattctgc gtgtactctt tatcgacgag ccatttcatg atgaatggct
     3421 tgaagagttc gagagccatt ttcttgggga tcccgcactg ataaatttta agttccggcc
     3481 c
//
LOCUS       DAOMPD010000283         3476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11086_length_3476_cov_2.721426, whole genome shotgun sequence.
ACCESSION   DAOMPD010000283 DAOMPD010000000
VERSION     DAOMPD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11086_length_3476_cov_2.721426"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PLY93_09820"
     CDS             <1..927
                     /locus_tag="PLY93_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69814.1"
                     /translation="EAKRLYQSQALVVERYLEKARHVEVQILGNAAKENFALFDRDCS
                     AQRNNQKIIEEAPAPHLPNTVRLTLHASAVRLANHVGYTNAGTMEYLYDSARQECYFL
                     EMNTRLQVEHTVTEMITGLDLVYEQIRIAEGARTDYSTVKAFGHAIEARICAEKSDGS
                     YQPSTGVITQYIEPKEQPPVACEPGASMRLESGVELGSRVSGKYDNMIAKLIVHAENR
                     DAARVFLHAKLTEFIINGLHTNIPLVKALLLDSKFAAVEHYTRYLQREFTPPQNDVEE
                     TATVAALLLYEIERNESTTRIDTILETHKQRV"
     gene            872..1489
                     /gene="lptE"
                     /locus_tag="PLY93_09825"
     CDS             872..1489
                     /gene="lptE"
                     /locus_tag="PLY93_09825"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRP69815.1"
                     /translation="MKVQLASTQFWKHINSVCKIFRKIYPAFLVYLAACASLAQGNGT
                     VNSQPLTDAYKNYRSQKKHSLYIQNFDNRTYAPQLTGRLKAKLQIALASSASLYITDD
                     KNNADLVLYGKILLYGEEPTQFSAMNQAPAFNLVIAASIKLRARVEEKAAQEEILYEQ
                     HDIQFSTMYNTGDPYFETRFTAEERLLDGLANRIANIIFDPKVKP"
     gene            1486..1947
                     /locus_tag="PLY93_09830"
     CDS             1486..1947
                     /locus_tag="PLY93_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HRP69816.1"
                     /translation="MILATLCYVRSNGKTLMMHRNKRVDDVHFGKYNGLGGKFEPMES
                     PEECVVREVFEESNLRITPRLKGVMTFPQFAKGHDWYCFLYTADTFTGTLKDDCPEGD
                     LVWIDNNKICDLPLWPGDKIFLSWLDSSHFFSAKFNYENGELKNHAVVFYG"
     gene            1940..3247
                     /locus_tag="PLY93_09835"
     CDS             1940..3247
                     /locus_tag="PLY93_09835"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRP69817.1"
                     /translation="MAEQEKLTTTAWGRFLKMGALAGKVGFSLVGEKALGLFLSDDSR
                     EMRMAKSWAKNAEKIAETLGQLKGGAMKIGQMLSIQEALLPKELTFVLKSLQNNAPTI
                     SADTMFQTLDADIPHWRTLIKSIDHKPLAAASIGQVHRATMLDGREVAVKIQYPKIDE
                     VIFSDLKNLRRLFELILESVMSANLTHLFTEIEARLSEEVDYRREKTRLEEFRRFYTK
                     NPKVSIPKPVPELSSQRVLTTELLLGLSIEEARHYPQEKKNEWGILLFQSLVEQLLVF
                     RKLQSDPNPANYAFKKDSGIILYDFGSIKELPDWLHQGYMGVVQIAREGQFEKIATIL
                     ASMQISLKNGDKIPQHQIDFFYETLSPIFTNKPFRFTKTTEPIQKIVAYNKSHFDELK
                     NIAIPGDVLFIDRAFMGLCANLTALEAEAHWGDVLLNAIDARV"
     gene            complement(3263..>3476)
                     /locus_tag="PLY93_09840"
     CDS             complement(3263..>3476)
                     /locus_tag="PLY93_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69818.1"
                     /translation="IVKKSMGKVPVALYVNYKDFANDQTRTKIADEYRQIFKPNLKDF
                     EQEFYRYLINTLEIKYYTYLKKNLKK"
BASE COUNT         1000 a          834 c          776 g          866 t
ORIGIN      
        1 gaagcaaagc ggctttacca aagccaagcc ttggtagtgg agcgttattt agaaaaggca
       61 cgccacgtcg aggtacaaat tttgggcaat gctgcaaaag aaaactttgc ccttttcgac
      121 cgcgactgta gcgcacagcg caacaaccaa aaaattatcg aagaagcccc tgcgcctcat
      181 ttacccaaca cagtacgttt gacactccac gcatcggcgg tacgcttggc gaaccatgtc
      241 ggttatacca acgcaggcac gatggaatac ctttacgata gcgcacggca agagtgctat
      301 tttcttgaga tgaacacccg cttacaggtc gagcacacgg taaccgaaat gattacggga
      361 cttgatttag tttacgaaca aatccgtatc gccgaaggcg cgcgtactga ctattctacg
      421 gtgaaagcat tcggtcacgc gattgaagcg cgcatctgcg cagaaaaaag cgacggcagt
      481 taccaaccct cgacgggagt gattactcaa tatattgaac cgaaggagca acctcctgtt
      541 gcgtgcgaac ctggcgcatc catgcgcctt gaatccggtg tggagttggg gtcgcgggtt
      601 tcggggaagt acgataacat gatcgcaaaa cttattgtgc acgccgaaaa ccgtgatgcc
      661 gcgcgggtgt tcttgcacgc caaactaact gaatttatta tcaacggctt gcacaccaat
      721 atacctctcg tcaaggcgct actccttgat tccaaatttg ctgccgtcga gcactataca
      781 cgttatttac aacgggagtt tacgccgccg caaaatgacg tggaagaaac ggcgacggtt
      841 gcggcgttac ttttgtacga gatcgagcgt aatgaaagta caactcgcat cgacacaatt
      901 ctggaaacac ataaacagcg tgtgtaaaat ttttagaaaa atttaccctg cttttttggt
      961 atatctcgct gcgtgcgcat cgctcgcaca aggaaatgga acggtgaact cgcagccttt
     1021 gaccgatgca tacaaaaatt atcgctcaca aaaaaagcac tcgctctata tccaaaattt
     1081 tgacaaccgc acatacgcgc cacagctcac ggggcgactt aaagcaaagt tgcaaatcgc
     1141 actagcgtcc agcgcatcgc tttatataac cgacgataaa aacaatgcag atttagttct
     1201 gtatggaaaa attttactct acggcgaaga acccactcag ttctcggcga tgaaccaagc
     1261 accggcgttt aatttagtga ttgctgcctc aatcaaatta cgcgcacgcg ttgaagaaaa
     1321 agcagcgcaa gaagagatac tctacgagca gcacgatatt caattttcaa cgatgtacaa
     1381 cactggcgac ccctattttg aaacccgctt taccgccgaa gaacgactcc tcgacggact
     1441 tgccaaccgc atcgccaaca ttatttttga ccccaaggta aaaccatgat cttagcaacc
     1501 ctctgttatg tacgttccaa cggtaaaact ctgatgatgc accgaaacaa gcgcgtcgac
     1561 gatgtacact ttggtaagta caatggtttg ggtggaaaat ttgaaccgat ggaatctccc
     1621 gaggaatgcg tggtgcgcga agtttttgag gaaagcaatc ttcgcatcac gccccgcctt
     1681 aaaggggtga tgacgtttcc acaatttgcc aaaggacacg attggtattg ttttctttat
     1741 actgccgata cgttcactgg cactctcaaa gacgattgcc ccgaaggcga tcttgtgtgg
     1801 atcgacaaca ataaaatatg cgacttgcca ctttggccgg gagataaaat ttttctttcg
     1861 tggctcgatt catcgcattt tttttcggca aagtttaatt acgaaaacgg agagttaaaa
     1921 aaccacgcgg tagtttttta tggctgaaca agaaaaactc actacaaccg catggggtcg
     1981 ctttttaaaa atgggagctc tcgctggcaa agtcggattc tctctggtag gagaaaaagc
     2041 gttgggtctt tttctcagcg acgactcacg cgaaatgcgc atggcaaaat catgggcaaa
     2101 gaacgccgaa aaaattgccg aaactctcgg ccaattaaaa ggcggcgcga tgaaaatcgg
     2161 ccaaatgctt tcaatccaag aagcgttact gcccaaagag ctaacttttg ttttgaaaag
     2221 tttgcaaaac aacgcaccga cgatttccgc cgacacgatg tttcaaacgt tagacgccga
     2281 catcccccat tggcgcactc tcattaaaag catcgaccac aaaccgctcg cggcggcgtc
     2341 gattggccag gtgcatcgtg caacgatgct cgatggccgt gaagtagcgg taaaaatcca
     2401 ataccctaaa atcgatgaag ttattttttc agacctgaag aatttacgcc gccttttcga
     2461 gctcattctc gaatcggtga tgtcggccaa tttaacgcac ctttttacag aaattgaagc
     2521 gcggctttcg gaagaagtcg attaccgccg cgagaagacg cgcttagaag agttccgccg
     2581 cttctacact aaaaatccca aggtgagtat ccctaaaccg gtgcccgaac tttcatcgca
     2641 acgggtattg acgaccgaat tactattggg actttccatc gaagaggcga ggcattatcc
     2701 gcaagagaaa aaaaacgaat ggggcatttt acttttccaa agccttgtcg agcaacttct
     2761 tgtttttcgt aaattgcagt cagaccccaa ccctgcaaac tacgctttca aaaaagacag
     2821 cggcatcatt ctatacgatt ttggcagcat caaagaatta cccgattggc tccaccaagg
     2881 ctatatggga gtcgttcaaa ttgcgcgcga gggccagttt gaaaaaattg caactatttt
     2941 agcgtcgatg cagatttctc tcaaaaacgg cgataaaatt ccgcagcatc aaattgattt
     3001 tttttatgaa acgctttctc ctatttttac gaataaacca tttcgcttca ccaagactac
     3061 cgagccgata caaaaaatcg tcgcttacaa caaatctcat ttcgacgaat tgaaaaatat
     3121 cgccatccca ggcgatgtac tttttattga ccgtgcgttc atgggacttt gcgcaaattt
     3181 aaccgcactt gaagccgaag cgcattgggg agatgtcctt cttaacgcaa tcgacgcgcg
     3241 ggtctaataa ttcacaggca cgctattttt ttaagttttt cttgagataa gtgtaatact
     3301 taatttccaa agtgttgata aggtagcggt agaactcttg ctcaaaatct ttcaagttgg
     3361 gcttaaagat ttggcggtat tcgtcggcaa ttttggtgcg ggtttggtcg ttcgcgaaat
     3421 ctttgtagtt gacgtaaagc gcaacgggca ccttgcccat cgattttttg acaata
//
LOCUS       DAOMPD010000284         3468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11119_length_3468_cov_2.437738, whole genome shotgun sequence.
ACCESSION   DAOMPD010000284 DAOMPD010000000
VERSION     DAOMPD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_3468_cov_2.437738"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>268
                     /locus_tag="PLY93_09845"
     CDS             <1..>268
                     /locus_tag="PLY93_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP69819.1"
                     /translation="EAFIDRLAHASLVDGVRLSGAKKIYFRHNDLIHLEALLQKSKAS
                     RKMIITESLFSMDGDRAPLHELQELSQKYDALFYVDDAHALGLYG"
     assembly_gap    269..297
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <298..756
                     /locus_tag="PLY93_09850"
     CDS             <298..756
                     /locus_tag="PLY93_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP69820.1"
                     /translation="SVDFRMATLGKALGLEGAMLATTHNARKYLLHKARPFVFSTAPL
                     PAIAHAGLTAISLAGSMDSERAHVASLSGRVRALLQQKGFSTGSSTTHIIPLLFNSEI
                     EALWYADTLEKRNLHVKAIRPPTVPTPRLRFSMNARVSENVLFQLDEAFA"
     gene            757..1407
                     /gene="bioD"
                     /locus_tag="PLY93_09855"
     CDS             757..1407
                     /gene="bioD"
                     /locus_tag="PLY93_09855"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRP69821.1"
                     /translation="MPLFIAGTGTNIGKTMFSTALMKRWARAQHLQYLKPIQTGSDDD
                     TQTVQKLSGLPDNFFLPNIYRFSLAASPHLASESERKSIDIVHLKASLYALKETRTVI
                     ELAGGLMVPLTREGYYTNCDLMRDVGFPAVLVASTALGTINHTVMSWKILKNYDIECR
                     GFFFIHHAIKTRHGALLQRDNIRTIIEMTGAINLGLYSTEDLIGGDSYPPIPNLFL"
     gene            1404..2696
                     /gene="bioA"
                     /locus_tag="PLY93_09860"
     CDS             1404..2696
                     /gene="bioA"
                     /locus_tag="PLY93_09860"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRP69822.1"
                     /translation="MIWYPFTPQNGALNPILIQRGEREYLYDENGKAYIDAVASWWTM
                     AHGHNHPAIMNAIREQTQKLDHVMLADFTHEGALSLAEKLLTSTKKKFTHVFYSDNGS
                     TAVEAMLKLAVQYWSNEGHPAKNQFIKFDVNYHGDTVGAMATAAPSVFNRVFSPLLFH
                     APCFRYPRVGEDTLNAQMLIALEEYLEKNQEEIAALILEPLIAAAGGMVFECTDALNT
                     IAALAQKYNVLLLFDEVFTGMGRTGEMFAYQKAGVVPDILALAKGLTGGTLPLAATLV
                     NARVHRAFQTDDPLKTFYHGHTMTGNPIAVSAALASIQIFEKENTLQYVQALEQKMQL
                     LCSRLTKKFPQRIKNVRVLGAVAAIDIVASGEEGYVFSYAKEIKAQAFREGVILRPLG
                     NVLYVTPPYRISDAALEKTFLVIEEILENYAVDHPKFD"
     gene            2668..>3152
                     /locus_tag="PLY93_09865"
     CDS             2668..>3152
                     /locus_tag="PLY93_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP69823.1"
                     /translation="MPSTTPSLINRETALAILSGELPLYRALSDAAEAREKYFGKTVR
                     IQILDNIKNGACAENCCYCAQRSNATGVENYPLKDAEAIFADAQAAKEAGAYRFCMVT
                     SGTAPSMNTTEKLTRIIRRITEELNLRVCLSAGFLDLPKAVQLKEAGLDRYNHNLNTS
                     E"
     assembly_gap    3153..3189
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3190..>3468
                     /locus_tag="PLY93_09870"
     CDS             <3190..>3468
                     /locus_tag="PLY93_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004777846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRP69824.1"
                     /translation="KKICTTHTFADRVDTIETLNRAGVSLCSGVIIGMGESREDIIDV
                     AFKLKALSIVSIPVNFFIPVPGHAVENPSLLTPEFCLRALIVFRLVNP"
BASE COUNT          879 a          834 c          831 g          858 t
ORIGIN      
        1 cgaggcgttt atcgatcgtt tagcgcacgc ctctctcgtc gatggagtgc gcctttcggg
       61 ggcaaagaaa atatattttc ggcacaacga tttaatacac ttggaagcgt tgcttcaaaa
      121 aagcaaagcc agtagaaaaa tgatcatcac cgaatctctt tttagtatgg acggcgatag
      181 agcccctctt cacgaactgc aagagctttc acaaaaatac gatgcgcttt tttatgtcga
      241 cgatgcacac gctttgggac tctatggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc
      301 gtcgatttcc gtatggcgac gctgggaaag gcgttggggc ttgagggcgc gatgcttgcc
      361 accacgcaca acgcgcgtaa gtatttactc cacaaagcgc gtccgtttgt tttttccaca
      421 gcgcctttac ccgcgattgc gcacgcgggc ttgaccgcaa tttctttggc cgggtcaatg
      481 gacagcgaac gcgcacacgt cgcttccctt tctggtcgcg tgcgtgcgct gttgcaacaa
      541 aaaggctttt cgacgggcag ttcaacgacg cacattatcc ctctattatt taattcagaa
      601 atcgaagcgc tctggtatgc cgatacactc gaaaagagga atctacatgt aaaagccatc
      661 cgaccaccga cggttcccac gccgagattg cgtttttcca tgaacgcccg cgtctcagaa
      721 aatgtgctat tccaactcga cgaggcgttt gcgtaaatgc cgctttttat agcaggtacc
      781 ggcacgaata tcgggaagac aatgttttca accgcgctga tgaaaagatg ggcgcgtgcg
      841 caacatctcc aatatctcaa acccatccaa accggtagcg acgacgacac tcaaacggtg
      901 caaaaacttt ctgggcttcc cgataatttt tttctgccta acatttatcg cttttctctt
      961 gccgcctcgc cccacctggc ttctgagagt gaaaggaaga gcatcgatat agtgcacctc
     1021 aaggcatcgc tctatgcgct caaagaaaca cgcacggtga ttgaacttgc cggcggtctt
     1081 atggtgccgc tcacacgcga gggttattac acgaactgtg atttaatgcg tgatgtggga
     1141 tttccagcgg tcttggttgc ctctaccgcg ttgggtacaa ttaaccatac ggtgatgtct
     1201 tggaagatat tgaaaaatta cgatatcgaa tgccgtgggt ttttttttat tcaccatgcc
     1261 ataaaaacgc gccatggcgc gcttctacag cgagataata tccgcactat aattgaaatg
     1321 acaggcgcga ttaatttggg attgtattcc actgaggatt tgattggggg ggatagttat
     1381 ccacctatac ccaatttatt tttatgatct ggtatccatt caccccccaa aatggcgcgc
     1441 taaatcccat tttgattcag cgcggcgaga gagagtattt gtacgatgaa aacggcaaag
     1501 cgtatatcga cgccgtcgcc tcgtggtgga cgatggcgca tggacacaac catcctgcga
     1561 ttatgaatgc catccgcgag caaacgcaaa agctcgacca tgtgatgctc gccgatttta
     1621 cgcacgaggg cgcgctatcg ctggcagaaa agttacttac aagcacgaaa aaaaaattta
     1681 cgcacgtttt ttattccgat aacggctcca ccgccgttga ggcgatgctc aagctcgctg
     1741 tgcagtattg gtctaacgaa ggccatcccg ctaaaaatca atttatcaag tttgatgtaa
     1801 actaccacgg cgacacggtg ggggcaatgg cgaccgctgc ccccagtgtt tttaaccgcg
     1861 tgttttcgcc gcttttattc cacgcgccat gttttcgcta tcctcgtgtg ggagaagata
     1921 ctctcaatgc gcagatgctc atagcgctgg aggagtatct cgaaaagaac caagaagaaa
     1981 ttgccgcact tatccttgaa ccactcatcg ccgctgcggg cggtatggtt tttgagtgta
     2041 ccgatgcgct caataccatc gccgcacttg cgcaaaaata caacgtcctt ttactttttg
     2101 atgaagtctt tactggtatg gggcgcacag gggaaatgtt tgcgtaccaa aaagcgggcg
     2161 ttgtgcccga catacttgct ctggccaagg ggctcaccgg cggcacgctc cctttggctg
     2221 cgacgctagt gaacgcgcgt gtgcacagag cattccaaac ggacgatcca ttaaaaacat
     2281 tttaccacgg gcacactatg accggcaacc cgattgcagt gagcgcggcg ttagcgtcca
     2341 tccaaatttt tgaaaaggaa aacacgttgc aatatgtcca agcgttggaa caaaaaatgc
     2401 agctgctttg tagccgtctc acaaaaaaat ttccgcagag aataaaaaat gtgcgtgtct
     2461 tgggcgccgt tgccgctatc gacattgtgg cctctggtga ggaagggtat gttttttcgt
     2521 atgcaaaaga aatcaaagcg caagcgttta gagaaggggt aatcttacgt ccattgggga
     2581 atgtattata cgtgacaccg ccttaccgga tttcagacgc tgcacttgag aaaacatttt
     2641 tagtgattga agaaattttg gagaactatg ccgtcgacca ccccaagttt gattaaccga
     2701 gaaaccgcac tggcaatttt atcgggagag ttgccgcttt atcgagcgct gtccgacgcc
     2761 gcagaagcgc gcgaaaaata ttttggaaaa acggtgcgca tccaaatctt ggataatatc
     2821 aaaaacggcg cctgcgccga gaactgctgc tactgcgctc agcgttcaaa tgccaccggc
     2881 gtggaaaatt atccacttaa agacgcagaa gcaatttttg ccgatgcaca agcagcgaaa
     2941 gaggcggggg cgtaccgttt ttgtatggtg acatctggca ctgcgccatc gatgaacacc
     3001 accgaaaagc ttacacgtat tatccgccgt atcaccgagg aattaaattt gcgcgtgtgc
     3061 ctctcggcgg gattcttaga tttacctaaa gcggtgcaac ttaaagaagc gggcctcgac
     3121 cgttacaatc acaacctgaa cacctcagaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnna taaaaaaatt tgtaccacac acacgtttgc agacagggtt gacacgatag
     3241 agacattaaa tcgtgcgggt gtgtcgctct gctctggggt gattatcggt atgggtgaga
     3301 gtagggaaga tattatcgat gtggcgttca agctcaaagc gttgagcatc gtttcgatcc
     3361 ctgtgaactt ttttattcca gtgccaggac atgctgtaga aaatccttct ttgcttactc
     3421 ccgagttttg tctgcgtgca ctcattgtct tccgtttagt aaatcccg
//
LOCUS       DAOMPD010000285         3443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11216_length_3443_cov_2.899941, whole genome shotgun sequence.
ACCESSION   DAOMPD010000285 DAOMPD010000000
VERSION     DAOMPD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11216_length_3443_cov_2.899941"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1224
                     /locus_tag="PLY93_09875"
     CDS             121..1224
                     /locus_tag="PLY93_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRP69825.1"
                     /translation="MDILDRYIFKSFIKTFLGTLLLLFGLAIIVKIIDSLKMFTDNKH
                     GAWLLVKYYIVVSPSFVTFIVPPALMFAIAFSVAQFNRHFELTVIMTAGRSFKRILRP
                     MLIFSIFLSVAFFLFNEYVAYPASYKAVNISYILRDRGADARLKKSDKSSDLTLRFGN
                     RYYTIGNGLWYESRLIGFHLVEMTPQGTIARIISADAASVSAENSHSWSLKNVEITLF
                     NEQGAYVGMETFDAQQIELPETLRSFRNFYIETDPDERSIFEAYQLYRNRKELGGSYR
                     EFLTETVWHAGYPLICIFIVFLGGMTGGKLKKASAATSIAASMLFTLFYFFLMYFGTA
                     FGESGTLPPLIAGNLANFVAAGGAIWIHYKIDF"
     gene            1197..>2375
                     /locus_tag="PLY93_09880"
     CDS             1197..>2375
                     /locus_tag="PLY93_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69826.1"
                     /translation="MDSLQNRFLTMKNFYHKHPLPVILCVGFLLRALAAFYNYTPTAE
                     DDYANVIEPALAHYQTGAPIQTEAYRLAFLPKAFYAFIQPLKIFNVTSAPFLVSWGFF
                     ILGCFSLLGIWAFYRLGGEIGSEKENMRTTAAWLYAAHFILPFFSTRAFQESLTLTTV
                     PLALWFLLKKNARARDFLLGGVFLGLTVVFRFQVGILALTAFSFFVVYVFKKKITAKH
                     LLGYITGGLLALFFLIFLDKIEGRVLLSTPLEYIRINYEGNIASASYGSAPWHTYITL
                     LAAIFIPPFSLVLLYPFFAAFQKNLFLAINLVVFILLHSFIGNKLERFMLPVLPFFFL
                     LTLHGLFTLRKMRIVRYAWRGFVVLNALILLPVTLSRSQLNTIDAAAYLAHKPKDTKM
                     I"
     assembly_gap    2376..2385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2386..2704
                     /locus_tag="PLY93_09885"
     CDS             <2386..2704
                     /locus_tag="PLY93_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69827.1"
                     /translation="YRIDFWRQAYMSFAMPHPISVETLPALKAQVDITLQKPIYILSL
                     GEFTQSELTILKESGKICAIEHRFEPSWQEKLVIYANPKFNARRDTTVLYYCSPSLAR
                     ASK"
     gene            complement(2679..>3443)
                     /locus_tag="PLY93_09890"
     CDS             complement(2679..>3443)
                     /locus_tag="PLY93_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRP69828.1"
                     /translation="RAGADLVVGTRKANGGQIENWSLLRRFISWGATKMAHFFLPQNS
                     SDPMSGFFAVSKNFYNRIATEINPRGFKILLEILAHARGANMQEVGYSFRPRQFGESK
                     LSVSVAFTYLVALYDLKFGRLIPVRFIKYALVGASGVVVNQLVLFLSRSGFKLSDAIA
                     LGVALEISIFWNFIVNNYFTFKDKSIAGVKNLLWGFLKFNFVCLGGAFINYSITMSLV
                     ASFMRNIYLANLVGIGVSTIWNYFINLQITWKRELD"
BASE COUNT          875 a          836 c          707 g         1015 t
ORIGIN      
        1 cggtgtcggt ttttattttt gggtgactgc ctgcgacaat gcgtggaata ccacccaaga
       61 acgcgccgac cacgaatcgc aaccatcggc ggcagtgttt gtacggccac gttaaatttt
      121 atggacattc tcgatcgtta tattttcaaa tcgtttatca aaacattctt aggcaccttg
      181 ctcttacttt ttggcctcgc cattattgta aaaattatcg actcgcttaa aatgttcacc
      241 gacaacaagc acggcgcttg gcttctggtg aaatactaca tcgtggtgag cccgtcgttt
      301 gtgacgttta tcgtgccgcc cgcgctcatg ttcgccatcg cgtttagcgt cgcgcagttc
      361 aaccgccatt ttgaactcac ggtcatcatg acggcggggc gttcgttcaa gcgcatactg
      421 cgccccatgc ttattttttc tatttttctt tcggtcgcgt tttttctttt taacgagtat
      481 gtggcgtatc cggcatcgta taaagcggtc aatattagtt acattttgcg cgatagaggt
      541 gcggatgcac gtcttaagaa atcggacaag agcagtgatt taacgctgcg ctttggaaat
      601 cgctactaca ctatcggcaa cggactctgg tatgaaagcc gactcattgg ttttcatttg
      661 gttgaaatga ccccccaagg cacaatcgcc cgcattattt ctgccgatgc ggcgagcgtt
      721 tctgcagaaa atagccattc gtggtcgctt aaaaatgtag agattacgct tttcaacgaa
      781 caaggcgcgt atgtcggtat ggaaactttc gatgcacaac aaatcgaatt gcccgaaacc
      841 ttaaggtcgt ttcgtaactt ttatattgaa accgaccccg acgagcgatc tatttttgaa
      901 gcgtatcaac tctatcgtaa tcgaaaagaa ctcggcggct cgtatcgtga atttcttacc
      961 gaaacagtct ggcacgcggg ttatcctctc atctgtatat tcatcgtctt tttgggtggt
     1021 atgactggcg gtaagctgaa aaaagcaagt gctgcaacga gtattgctgc ttcgatgctt
     1081 tttactcttt tctatttctt cttgatgtac ttcggtaccg cattcggcga gagcggcact
     1141 ctaccgcctc ttatcgctgg caatttagca aattttgtcg ccgccggtgg agcgatatgg
     1201 attcactaca aaatcgattt ttaactatga aaaattttta ccacaaacat cctctccccg
     1261 tcattttatg cgtgggtttt cttttgcgag ctttggcggc gttctacaac tacacaccca
     1321 ccgccgaaga cgattatgca aacgtgattg aacctgcact cgcgcactac caaaccggcg
     1381 cccccattca aactgaggca taccgtttgg cttttttacc gaaagcattt tacgcgttta
     1441 ttcaaccttt aaaaattttt aatgtgacga gcgccccatt tctcgtttca tggggatttt
     1501 ttattttagg atgtttttct ttgttaggta tttgggcatt ctaccgttta ggtggtgaaa
     1561 tcggcagtga aaaggaaaat atgaggacaa ccgccgcttg gctctatgca gcgcacttta
     1621 ttttaccgtt cttttcaacg cgtgcgtttc aagaaagtct aaccctcacg accgtgccgc
     1681 ttgcgctgtg gtttctcttg aaaaaaaatg cacgcgcgcg tgactttctt ttaggcggcg
     1741 tgttcttggg gctcactgtt gtttttcgtt ttcaagtcgg tatcctcgcg ctcaccgctt
     1801 tttctttctt cgtcgtctat gtattcaaaa agaaaattac cgccaaacat cttttaggct
     1861 acataaccgg cggattactt gcattgtttt ttttaatttt tttggataaa atagaaggtc
     1921 gcgtattact ttcaacgcca ttagaatata tacgtattaa ctacgaaggc aatattgcca
     1981 gcgccagtta cggctctgct ccttggcata cgtatatcac tcttcttgcc gcgattttta
     2041 ttccgccgtt ttctctggtg cttttatatc cgttctttgc cgcgtttcaa aaaaacttat
     2101 ttttagccat caatcttgtc gtgtttattt tactacacag ctttatcggc aataaacttg
     2161 aacgctttat gctaccagtg ttacccttct tttttctgct tactctccac ggattattca
     2221 cattacgtaa aatgcgtatc gttcgctatg cgtggcgtgg ttttgtcgta ctcaatgcac
     2281 ttatactttt acccgtaacg ctcagtcgta gccaactcaa caccatcgac gcggcggcgt
     2341 atttggcaca caaacctaaa gacacgaaaa tgattnnnnn nnnnngtatc gcatcgattt
     2401 ttggcgacaa gcgtatatgt cgtttgctat gcctcacccc ataagcgtcg agacgctacc
     2461 tgcgttaaaa gcacaggttg acataacgct gcaaaagcct atttatattc tctctttagg
     2521 tgaatttaca caaagcgaac tcaccatcct aaaagagagc ggtaaaatct gcgccatcga
     2581 acatcgtttt gagccttcgt ggcaagaaaa actggtgatc tacgctaacc caaaattcaa
     2641 tgcacgccgc gacacgacgg tactctatta ttgctcccct agtctagctc gcgcttccaa
     2701 gtgatttgca ggttgatgaa gtagttccaa attgtcgaaa cgccgatacc cactaaattg
     2761 gctaagtaga tattgcgcat aaaacttgcc acaagactca tcgtgataga atagttgata
     2821 aaagccccac ccaaacaaac aaaattgaat ttaagaaatc cccatagtaa attttttacg
     2881 ccagcaatac ttttatcttt gaacgtgaaa taattattca cgataaaatt ccaaaagata
     2941 gaaatttcaa gcgcaacgcc caacgcaatc gcatccgaaa gtttgaaccc actacgcgaa
     3001 agaaagagca cgagttgatt caccacaaca cccgacgcac ccaccaacgc atatttgatg
     3061 aagcgcaccg gaatcagccg accaaacttg aggtcgtata gcgcaacaag atacgtaaac
     3121 gccaccgata cgctcagctt gctttcacca aactggcgtg ggcgaaaact atatcctacc
     3181 tcttgcatgt tggcgccgcg tgcatgcgcc aaaatttcga gaaggatttt gaaaccgcgc
     3241 gggtttatct cggtggcaat gcggttatag aaattttttg atacggcaaa aaaaccgctc
     3301 atcgggtcac tcgagttttg cggtaaaaaa aagtgcgcca tcttcgtcgc tccccatgaa
     3361 ataaaacgac gtaggagcga ccaattctca atttggccac cgttcgcttt gcgggtaccg
     3421 acgactaaat ctgcccccgc gcg
//
LOCUS       DAOMPD010000286         3439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11233_length_3439_cov_2.195035, whole genome shotgun sequence.
ACCESSION   DAOMPD010000286 DAOMPD010000000
VERSION     DAOMPD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11233_length_3439_cov_2.195035"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLY93_09895"
     CDS             complement(<1..434)
                     /locus_tag="PLY93_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69829.1"
                     /translation="MNQTRTKSFSWILIICLGIIACKKTSLFTEIDARFKKLDDVFVE
                     EFKNEKRVIENNTEQNLNAGEAKTGRYGTLLVLDVWEFPQADDSKRNYEALVEEEKRN
                     TPREYDQTKANEYRYQFVRGSGVAGEIFTIKKLLFRVLGENK"
     gene            complement(431..1090)
                     /gene="rpe"
                     /locus_tag="PLY93_09900"
     CDS             complement(431..1090)
                     /gene="rpe"
                     /locus_tag="PLY93_09900"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRP69830.1"
                     /translation="MSVRISPSILAAPLAYLGQAAKQVEEARCDYVHIDVMDGHFVPA
                     LTFGDQITAAIGAETKLPLDVHLMVAKPEVEAQKYFALKPGILTFHYEATTAPIRLAQ
                     TIRAQGIHAGISLNPRTPVSVLNDLAPYFDVVLLMTVEPGFYGQKFIESSWQRLKELN
                     ALKTREKNAGRMLQIEIDGGVTDANAVQLAQEGADILVSGSYLFKDGMKERVQKLKGQ
                     V"
     gene            complement(1090..2247)
                     /locus_tag="PLY93_09905"
     CDS             complement(1090..2247)
                     /locus_tag="PLY93_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69831.1"
                     /translation="MNTTKNPSTATGPKLLFIKHLSKIALFYFVGLSIFFISGFLIFK
                     ILFADTEKAIVPDVVGRLFLGEHNKLREDFKIEITPTHLVEYPYGYILAQDLTPGKSV
                     DKNTKLELLVNLSDAIVQVPKLIGFSETLVDGSISSLPVGGRIFSLRKGVVTHIPSNQ
                     PKREVLAQFPPPGTPVLPNTPIALLVSDGPLPTAPKAPQALLQQKIEKGVPVSIALNA
                     AYHLKIPATIKLVKVEKAETNATLLTDAKISADSIELEVGEFITTPAKGKSKIPPNDL
                     PHAQLFVPADKWGTVNQVLTIARREKDLGEDGSPYSEYFLIKNGSPLPLFRRRNETFD
                     VYHDYYAYSAIKPAETTPEAGKTIIGDAPQVKEAPKERSPDKTVHLVAETL"
     gene            complement(2296..>3194)
                     /locus_tag="PLY93_09910"
     CDS             complement(2296..>3194)
                     /locus_tag="PLY93_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP69832.1"
                     /translation="FTLSYLAHEVLFVNNLYHSASDAQRKKFIPQVLDGTALAGMAMS
                     EPGAGTDVLGMTTSAVKEGDKYILNGVKQWITNGNVAQYFIVYARTDKNNRQAISSFI
                     VDSAMPGFSVGKKEIKMGMRQSPTCQLILEDVKVPQENLIGAEGGALTHMMRNLEIER
                     LTLAAMSVGIARRALDDMVRYASQERKAFGRPLADFGQIQQMIAESYARYRASRALVY
                     ETALRINPTSRESLSAAAAKLSATQMAEYVARSAIQVSGGYGYTREYAVEKLLRDAIL
                     LSIGGGTNEAMQKNIMKDLRGG"
     assembly_gap    3195..3220
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3221..3424)
                     /locus_tag="PLY93_09915"
     CDS             complement(<3221..3424)
                     /locus_tag="PLY93_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP69833.1"
                     /translation="MRALTHDLAQGVLEKQVKEFDEKETLNEALLRRMGSEYNFFGIT
                     IPTEAGGHGLDAAAAVIVHEELSY"
BASE COUNT          800 a          793 c          869 g          951 t
ORIGIN      
        1 tttttgtttt cgcctaaaac gcggaataat aatttcttaa tcgtgaaaat ttcacccgcc
       61 acaccactac cgcgtacaaa ttggtagcgg tattcgttcg cctttgtttg atcgtattcg
      121 cgcggggtgt tgcgcttttc ttcttcgacg agtgcctcgt agttacgctt gctgtcgtca
      181 gcttggggaa actcccacac gtcgagcacc aagagtgtac cgtaacgacc ggtcttggct
      241 tcacccgcgt tcaaattttg ttcggtatta ttctcaatga cgcgcttttc atttttgaat
      301 tcttccacaa aaacatcgtc gagttttttg aaccgcgcgt cgatttcggt aaaaagactc
      361 gtctttttac acgcaatgat gccaagacat attattaaaa tccaagaaaa actttttgtt
      421 cttgtctgat tcatacctgt cctttgagct tttgtacgcg ctctttcata ccgtccttga
      481 aaagataact cccagaaact aaaatatccg cgccctcttg ggcgagttgg actgcatttg
      541 catcggtgac accaccgtcg atttctattt ggagcatacg ccctgcattc ttttcacggg
      601 tcttgagcgc gttaagctct ttaagacgtt gccacgacga ttcgataaac ttttgaccgt
      661 aaaaaccagg ctcgacggtc atcaggagca ccacgtcgaa atatggcgca agatcattca
      721 agacggaaac cggtgtgcgt gggttgagtg aaatgccagc gtggatgcct tgtgcacgga
      781 ttgtctgggc taagcggatg ggggcggtcg ttgcctcgta atggaaagta agaatccctg
      841 gttttaatgc gaaatacttt tgcgcttcga cttcaggctt tgccaccatg aggtgcacat
      901 cgagagggag tttggtttcg gcgccaatcg cggcagtgat ttgatcgcca aaggtgagcg
      961 ccggtacaaa gtggccgtcc atcacgtcga tgtggacata atcgcagcgc gcttcttcga
     1021 cttgcttggc cgcttgcccc aaataggcta agggggcagc aagaatgctg ggggaaatgc
     1081 gaacagacat tagagtgtct cggcaacaag gtgcacggtt ttatcggggc tgcgttcctt
     1141 tggggcttct tttacttgcg gggcatcgcc aataatagtt ttgcctgctt ctggagtggt
     1201 ttcggcaggc tttatggcgc tgtatgcata atagtcgtgg tatacatcga aggtttcatt
     1261 gcggcggcgg aaaagcggta aaggggatcc attcttaatc agaaaatatt cgctatacgg
     1321 tgagccatct tcccctaaat ctttttcgcg gcgggcaatc gttaaaactt gatttaccgt
     1381 tccccattta tcggcgggaa caaaaagctg cgcatgcggc aaatcgttcg gggggatttt
     1441 tgattttcct tttgcaggcg tcgtgataaa ttcacccact tcgagttcaa tggagtctgc
     1501 tgaaattttt gcatcggtaa gcagggttgc atttgtttcg gctttttcta cttttacaag
     1561 tttaatggtt gccggtattt taagatggta agcggcgttg agtgcgatag aaaccggaac
     1621 tcctttttca attttttgtt gtaagagcgc ctgtggcgct ttaggcgcag taggtaaagg
     1681 accgtcggag accaggagag cgattggagt gtttggtaaa acaggagtgc ccggaggggg
     1741 aaactgtgcc aacacttcac gcttgggttg gttggacggg atatgcgtta ccaccccttt
     1801 gcgcagtgag aaaatccgcc caccgacggg taggctacta atcgagccat cgactaatgt
     1861 ttctgaaaaa ccgatgagct tgggcacttg cacaatggca tcggataaat taacaagtaa
     1921 ttccaattta gtgttcttat caactgattt acccggggtt aaatcttgtg caagaatata
     1981 tccgtacggg tattcgacga ggtgggtggg ggttatctct attttaaaat cttcgcgcaa
     2041 tttattgtgt tcacccaaga aaagacgacc aaccacatca ggaacgatgg ctttttctgt
     2101 gtctgcaaaa agaattttga aaattagaaa tccggaaatg aaaaagatgg agagacccac
     2161 aaaataaaaa agggcaattt tagaaagatg ctttatgaaa agtaatttag gtcccgtggc
     2221 cgtcgacgga tttttagtag tgttcatttc agggtgagtt tgggcggata aatcaagctg
     2281 taaagaattt gtgcgttaac ctccacgtaa gtctttcata atattcttct gcattgcctc
     2341 gttggtgccg cccccgatgg aaagtaagat cgcgtcgcgt aacagttttt caaccgcgta
     2401 ttcacgggtg tagccatagc caccagaaac ttggattgcc gagcgcgcca cgtattcggc
     2461 catttgagtc gccgaaagtt ttgccgccgc cgctgagagc gattcacgcg atgtggggtt
     2521 tattctgagt gcggtttcat acacgagcgc acggctggcg cggtaacgtg cgtaagactc
     2581 ggcgatcatt tgctggattt ggccaaagtc ggcaaggggg cgaccaaacg ctttgcgctc
     2641 ctggcttgca taacgcacca tgtcgtcgag cgcacgccgg gcaatcccca ccgacatcgc
     2701 cgcgagcgtc aaccgttcaa tttctaaatt gcgcatcatg tgtgtgaggg ctcctccctc
     2761 ggcgccgatg aggttttctt ggggaacttt aacatcttct aaaataagtt ggcaagttgg
     2821 ggattgtcgc atgcccattt tgatttcctt tttcccgacg ctaaaacccg gcatagcgct
     2881 atcgacaatg aagctggaga tggcttgccg gttgttttta tcggtgcgtg catagacgat
     2941 aaagtactgc gcgacattgc cgttagtgat ccactgcttg actccgttta agatgtactt
     3001 atcgccttct tttaccgcac tggttgtcat acccaggacg tcggtgcctg caccgggctc
     3061 agacatcgcc atgccagcga gcgcagtacc gtctaaaacc tgtgggataa atttcttgcg
     3121 ttgtgcgtcc gacgccgaat ggtataaatt attcacaaac aacacttcgt gcgctaaata
     3181 gcttaaagta aatgnnnnnn nnnnnnnnnn nnnnnnnnnn ataactcagc tcttcgtgga
     3241 caatcaccgc cgccgccgcg tcgagaccat gcccgcccgc ctcggtgggg atggtgatac
     3301 cgaaaaaatt atattcactc cccatacggc gtagcagcgc ctcgttgagc gtttcttttt
     3361 catcgaactc tttcacctgt ttttctaaaa cgccttgggc aaggtcgtgc gttaaggcgc
     3421 gcaatgccgc gtggctttc
//
LOCUS       DAOMPD010000287         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11304_length_3422_cov_2.117315, whole genome shotgun sequence.
ACCESSION   DAOMPD010000287 DAOMPD010000000
VERSION     DAOMPD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11304_length_3422_cov_2.117315"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1169
                     /locus_tag="PLY93_09920"
     CDS             108..1169
                     /locus_tag="PLY93_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69834.1"
                     /translation="MRLLTLFFFSLAIFALSAQEKPITTDKVYIGASEATTNEKSEEE
                     LQKARLLKSEKRQKLVLAAKLSEARSGAKHVQRADGSYCANSLAQFTLQGEDDISGLN
                     RFHYRLNDDAWLDYLGPVSIAQEGEHYLKWESIDRVGNREDQQSLKIRIDNTPPTLTP
                     TPVGRFVTGRNGYTAAPQFQLAVRAHDAGCGVQAVYMNLDNGGWTPLKGTALLFEKPG
                     THHVQLMAEDILNNRSAIKQMTVTIDATPPRLVILASPAPLVENGASVCRPGTVVSLA
                     AEDDETQVARIEYRYSPNNEWIGSMDTRQPLQDTREFFIEARAIDSVGNTTEQIPSFR
                     CRVWGEPPTTKIIMHGGDK"
     gene            1166..1330
                     /locus_tag="PLY93_09925"
     CDS             1166..1330
                     /locus_tag="PLY93_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69835.1"
                     /translation="MKAAPFVLFTAALGFMACGGLKGQIKESRAQSRSATAAAVYGEK
                     LMRKTITRRY"
     gene            1450..>2524
                     /locus_tag="PLY93_09930"
     CDS             1450..>2524
                     /locus_tag="PLY93_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69836.1"
                     /translation="MLADEESIYQITNDTKLQEFLKKIQQVRSEEAAKKYRVALASME
                     KISPLPDGSYYAVYRDALKAKAEKPEKDLNAILAALEKPDASPLTDAEAVYRLREAFA
                     VEPELAALEKYKNRYIKIIQNMNATVDCTASIKEPPGKVYMSTGYEWPLEVAVRCNNA
                     ALQSHLALRFDSAGPVSFGDSATKSVVLILDKLKGEFPVTELSVNKEENKFSLRGISR
                     FNETMAVLSVYAPKPTLAFRITPEEYTLKGVPVFYSESYDRQAAGAQPATPWKTLTGK
                     ETQATLAMNGQFTLGGQRDGAAYDMVYGHPNGKYSDSIWSSYITLSVDGKFYRFDKIP
                     GLTRSESADKKTLTTTGKLVEEDA"
     assembly_gap    2525..2534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2535..>3422
                     /locus_tag="PLY93_09935"
     CDS             <2535..>3422
                     /locus_tag="PLY93_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69837.1"
                     /translation="TVTLTLTRDGEDNYVVGVEYTQLSGSAKEVGVRLLVDTWAGKTD
                     GVPFLTPAGAGNASQIHTHEFKFTPAYSTLWETIDAAGDGYVYLRNTMSGKGLVPPDQ
                     IAFARWGSAYRSEWDYSVSSDNTLLGDSAAILWWQPIRVDSGKSRYIATRFGIFKRDE
                     KNIADVQDDKSGFTYLYLERKNESNAPRTYRIEIESPDAALQIPSGNEQTFTLQPQER
                     TVRAVPLTVVGGGEKKITVKEYHDNAKEPLVSSVVVKLAPETGRSANVPIWPNTKPYP
                     VRYISNNGDKKLRCVVKSAV"
BASE COUNT          922 a          900 c          870 g          720 t
ORIGIN      
        1 aaaacgcact caacttgacc ccaaaagtgt cagttatctc tgtgggttgc ataaggctcc
       61 ttagttatta gcttgacgtg aactttccta ttcaaaaata atataaaatg cgtttgttga
      121 ctctgttctt tttctctctt gctatctttg ctctcagcgc acaagaaaag ccgatcacca
      181 ccgacaaagt gtatatcggt gcgagtgaag cgaccacaaa cgagaaaagc gaggaagaac
      241 ttcagaaggc acggctcttg aaatccgaaa agcggcaaaa acttgtcctt gctgcaaaat
      301 tgtctgaagc gcgctcgggc gcaaaacacg ttcaacgcgc agacggttct tattgcgcca
      361 attcactcgc acaattcacg ttgcaaggcg aagatgacat aagcggactc aaccggttcc
      421 actaccgctt aaacgacgat gcttggctcg attatttagg tcctgtttcc atcgctcaag
      481 aaggcgagca ttatctcaaa tgggaaagta tcgaccgcgt cggcaaccgc gaagaccaac
      541 aatcgctgaa aatccgcatc gacaacacgc caccgacgct tacacccaca ccagtggggc
      601 gttttgtaac ggggcgcaat ggctacaccg ccgcgccgca atttcaatta gcggtgcgcg
      661 cacacgatgc cggttgcggc gtgcaggccg tctacatgaa tctcgataac ggaggatgga
      721 cacctttgaa aggcactgcc ttgctgtttg aaaaaccggg cacccaccac gtacaactca
      781 tggcagaaga tatattgaac aaccgctctg cgataaaaca aatgactgtc accatcgatg
      841 cgaccccacc tcggttagtc attttagcgt ctcccgcacc gcttgttgaa aatggcgcgt
      901 ctgtctgccg cccgggcacc gtcgtttcac tcgccgccga agacgatgaa acgcaagtcg
      961 cacgcatcga ataccgctat tcgcctaaca acgaatggat tggcagcatg gatacgcggc
     1021 aacctttaca agacacgcgt gagtttttta ttgaggcgcg cgccatcgac agcgtgggca
     1081 ataccaccga acaaataccg tcgttccgtt gccgcgtgtg gggcgagccg cccaccacca
     1141 aaattattat gcacgggggc gacaaatgaa agccgcacct tttgttttat ttaccgccgc
     1201 attgggtttt atggcttgcg gtggcctcaa aggacaaatt aaagaaagcc gcgcccagtc
     1261 gcgcagcgcc accgctgcgg cggtgtacgg tgaaaaattg atgcgcaaaa cgattacacg
     1321 tcgttattag aggaaatcgg acgcgaggat aatagctgca aaataaaaaa cgaaaaaaaa
     1381 accaactggg ttctctgctc ggccgatgcc aataatcaac tgagaggtat ttctgcatcg
     1441 tcgggagcgg tgcttgccga cgaagagtcc atctatcaaa ttaccaacga tacaaaacta
     1501 caagaatttt tgaagaaaat ccaacaggtg cgttcagaag aagcggcaaa gaaataccgt
     1561 gtcgcgctgg cgtcgatgga aaaaatttca ccgttacccg acgggtctta ttacgccgtg
     1621 taccgcgacg cgctcaaagc caaagccgag aaacccgaaa aagatttgaa cgcaatcctt
     1681 gcagcgctgg aaaaacccga cgcatcgccg ctcaccgacg ccgaggcagt gtaccgattg
     1741 cgcgaggcgt ttgcagtgga gcccgagctt gctgcgttgg agaaatataa aaatcggtat
     1801 ataaaaatta ttcaaaacat gaacgccacc gtcgattgca cggcttcgat taaagagccg
     1861 ccgggcaaag tgtatatgag caccggttat gagtggccgc ttgaggtggc ggtgcgctgt
     1921 aacaacgccg cgttgcaatc ccacttagct ttgcgcttcg attcggcggg gcccgtgagt
     1981 tttggcgatt cagcgactaa atcggttgtg ctcattttag ataaactcaa aggcgagttt
     2041 cccgtcactg agctttcggt aaataaggaa gaaaataaat tttcactgag ggggatttct
     2101 cgctttaacg aaaccatggc cgtgctgtcg gtgtacgcac ccaagcccac gctcgccttt
     2161 cgcatcactc ccgaagaata tacactcaaa ggcgtgcccg tcttttactc agaatcgtat
     2221 gatcgccaag cggcgggtgc gcagccggca accccgtgga aaactttaac gggaaaagaa
     2281 acccaagcga cgctcgcgat gaacggccaa ttcacgttgg gtggccagcg cgatggcgca
     2341 gcgtacgata tggtgtacgg ccaccccaac ggcaaatact ccgacagtat atggtcgtcg
     2401 tatatcacgc tgagcgtcga cggaaaattc tatcgcttcg ataaaattcc cggtctaacg
     2461 cgcagtgaat ccgcagataa aaaaacgctc acaaccacgg gtaagctcgt tgaagaagat
     2521 gcaannnnnn nnnnaaccgt gacgctcacg ctcactcgcg acggcgagga taactatgtc
     2581 gtcggcgtcg aatacacgca actgagcggt tccgccaaag aagtgggggt gcgtctactc
     2641 gtcgatacgt gggcgggtaa aacggacggc gtgccatttt taacgccggc gggtgcgggt
     2701 aacgcatcgc aaatacacac gcacgagttt aagttcaccc ccgcctattc gacgctttgg
     2761 gaaaccatcg acgctgcagg cgatgggtat gtctatttac gcaacaccat gagcggcaaa
     2821 gggcttgtgc cacccgacca aattgcattc gcgcgttggg ggtctgcgta ccgcagcgaa
     2881 tgggactatt ctgtgagttc cgacaacacg cttttgggcg actcggcggc gattttatgg
     2941 tggcagccca tacgtgtcga ttctggcaag tcgcggtata tcgccacacg ttttggcata
     3001 ttcaaacgcg atgaaaaaaa cattgccgac gtgcaagacg acaaatcggg ttttacgtat
     3061 ctctatcttg aaagaaaaaa cgaatcgaat gcgccgcgca cctaccggat tgaaatcgaa
     3121 agcccagacg ccgcgttgca aattccctcg ggtaatgagc aaacattcac tctacaaccc
     3181 caggaacgca cggtgcgcgc ggtacctttg accgtcgtgg gcggcggcga aaaaaagatt
     3241 acggtgaaag aataccacga caacgccaaa gaaccgcttg tttccagcgt cgttgtgaag
     3301 ttagcccccg aaaccggacg ttcagcgaat gtgcccattt ggccgaacac aaagccctat
     3361 cctgtgcgtt atatctcgaa caatggcgat aagaagttgc gctgcgttgt gaaatcggca
     3421 gt
//
LOCUS       DAOMPD010000288         3421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11310_length_3421_cov_2.701426, whole genome shotgun sequence.
ACCESSION   DAOMPD010000288 DAOMPD010000000
VERSION     DAOMPD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11310_length_3421_cov_2.701426"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..701
                     /locus_tag="PLY93_09940"
     CDS             165..701
                     /locus_tag="PLY93_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69838.1"
                     /translation="MRQSARALTIFISLVVLVGAGCTFERREDFNFSVDLPLRCGVFK
                     SYYFVRDVREEIYPDLALVLANANRLALTDQQREKLISAARHCNELCAVEKDTLRHMQ
                     EDIKAKLKLNEIHGDLNLLAKDVRAFERAKSTWLNHHAKRYYQGLSVLRSGQRALWVP
                     AEGFLNKLPSPESVGVYF"
     gene            780..1943
                     /locus_tag="PLY93_09945"
     CDS             780..1943
                     /locus_tag="PLY93_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69839.1"
                     /translation="MAAAIFLISRYRDGTPLIVVGFLFIHVFVSQPLKDWLIVRLHTS
                     SRAYPWIGSVNFENRMQRIMRMNDALDFLNWLIRHWKLSRLRVVLFDDKSIVYYLAAG
                     KKPRKMVVRDEIPEAFQQELLKNKSGAYTFNLSSPVKAYLQARKVKFVVPLLFRERLI
                     GVIGLNERLDKIRERFIVQAAQRISLAVENERLERTVPRSEFLKQEFRLAEKIERHLN
                     GVSEYRINGYTIQKLNTAWEKKHFTAIFGCAPGEGVHFVMLLRLERASTRSNALQLFT
                     MQGYFYSLSRGLTDVSQLADALHSAVVHNENQKFFVEGFLLSFSATMRITQLLAFGSH
                     LAYRGTTGWAWMPESPPLGSNGFSSKAVIEVVMTKDLVLSMREYPLVLIGSSV"
     gene            1940..>2760
                     /locus_tag="PLY93_09950"
     CDS             1940..>2760
                     /locus_tag="PLY93_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69840.1"
                     /translation="MIRFFWIFTYIVLLVIVPIQLVSTTDYIQRVKPRVLTTPHYLAV
                     VPLTFDIPDEASYLRSLESRVEDVSKLRYRQALLPIYAAAFFFWISGIFLYVFARVKR
                     VERAYILFSLFVTAYLLLFVDFLTTRYTDNFFFAYNIVIIAPFLYLYRSVYELETRGV
                     MYPIIMFIGVLSYFIFPVQSVSDEIFFIQLLGAIFTLVFFYCAYLFLRSEKKPKAEQA
                     VRKKWAARIFNISLISSVALPPAFFFTLYYFPVRLDINYNALFFLPAFFSIIFLTL"
     assembly_gap    2761..2802
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2803..>3421
                     /locus_tag="PLY93_09955"
     CDS             <2803..>3421
                     /locus_tag="PLY93_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69841.1"
                     /translation="TLSLRWGLVAFHVPISLWAVRLLYFAFFGFLYWFTIGFSLGEIF
                     QNETIRLGHFIVLGVFLILIDPLRTALLTSLDTYTHARRQSLDAFLSQSFQQISNPQR
                     VNQIVDRIATLLSEGLSASWAKVVVSNDLFRDWVVSSDKVVALPSDDPFWIEAYRPLR
                     KGRFLVLTRTFIGPVRDFLQKQGGYLVITMSKFKAGILIAERRENT"
BASE COUNT          815 a          677 c          791 g         1096 t
ORIGIN      
        1 tggagtggtc gaaaaggagg gtaaattttg aggaaaagct ctctttcagg cgggcgggac
       61 atcgcttctt cgcaacacga aggccatagc agcctggacg gattacagtt atggtataat
      121 cactttatga tttacacgca acgccacccc ccttatagga gtctgtgcgt caaagtgcaa
      181 gagctttaac gatttttatt tctttagttg tgctcgttgg ggcgggatgc acctttgaac
      241 ggcgcgagga ttttaacttt tcggtcgatt tacctctacg ctgtggggtg tttaagtcat
      301 attatttcgt acgcgatgtt cgtgaagaga tataccccga tttagcgctc gtgttggcga
      361 acgcgaaccg cttggcattg acggaccaac aaagagaaaa attgataagc gcggcaaggc
      421 attgcaatga actttgcgcc gtagaaaaag atacacttag acacatgcag gaagacatta
      481 aagcaaaact caagttaaat gaaattcacg gtgacttgaa cttactcgcc aaggacgtac
      541 gtgcttttga acgtgccaaa tcaacgtggc ttaaccacca cgctaaacgc tattatcagg
      601 gtttaagtgt tttacgcagt ggccagcgtg cgctttgggt acccgctgaa gggtttttga
      661 ataaattacc gtcacccgaa tcggtaggcg tgtacttcta aaatcctatg ttctggcata
      721 ggttaaaagc actcccttgg tgggcgatta gtttatcatt tggtgtcaat attattgcgt
      781 tggcagcagc cattttcctt atatcgcgct atcgtgatgg cacaccgctt atcgtcgttg
      841 gttttctttt tattcatgtt tttgtttcgc aaccattaaa agattggttg attgtgcgtt
      901 tgcatacttc gtcgagggca tatccatgga ttgggagcgt taattttgaa aatcgcatgc
      961 agcggattat gcgtatgaac gatgcgcttg attttttgaa ttggttaatt cggcattgga
     1021 aactttcacg tttacgcgtt gttcttttcg acgacaagag tattgtctat tatttagcgg
     1081 cgggtaaaaa accgcgcaaa atggtcgttc gcgatgaaat acccgaagca ttccaacaag
     1141 aattgttgaa aaataaatcg ggtgcctaca catttaattt atcatcgccg gttaaagcat
     1201 atttacaagc gcggaaagta aaatttgttg tgcccctcct tttccgtgaa aggctcattg
     1261 gcgtgattgg cctcaacgaa aggttagata aaatccgcga gcgtttcatc gtacaagcag
     1321 cgcagcgcat cagtcttgcc gttgaaaacg aacggcttga gcgcaccgtt ccccgcagcg
     1381 aatttttgaa gcaagagttc cgtcttgcgg aaaaaattga acgacacttg aacggtgtat
     1441 cagagtaccg tataaacggc tatacaatcc aaaaattaaa tactgcgtgg gagaagaaac
     1501 actttaccgc tatttttggc tgtgcgcctg gggaaggagt tcactttgtg atgcttttgc
     1561 gtttagaaag ggcttcaaca cgttcaaatg cgttacagct ttttacaatg caaggttatt
     1621 tttattcgct ctcacgcggg ttaaccgatg tctcgcaact ggcggatgcg ttacacagtg
     1681 cggtagtgca caatgaaaac cagaagtttt ttgttgaagg gttcctgctt tccttttctg
     1741 cgactatgcg cattactcaa ttattagcgt ttggcagtca tcttgcttat cgaggaacta
     1801 cgggttgggc atggatgcca gaaagtccgc ctttgggttc gaatgggttt tcgtctaaag
     1861 cggtaatcga ggtggtaatg acgaaggatc ttgttttaag catgcgcgaa tatcccctgg
     1921 ttttgattgg cagttcggta tgatacgatt tttttggata tttacttaca tcgttctttt
     1981 ggtgattgtg ccgatacagt tggtctccac aacagattac attcaacgag ttaagccacg
     2041 agttttaaca acacctcact atttagcggt ggtgccgttg acgtttgata tacccgatga
     2101 ggcgagttat ttacggtcgt tagaaagccg tgttgaagat gtgagtaaat tgcgttatcg
     2161 ccaggcactg ttgcccattt atgccgctgc attctttttt tggatttcag ggattttttt
     2221 atatgtattt gcgcgggtaa aacgtgtcga gcgtgcctat atccttttta gtctttttgt
     2281 aacggcgtat cttttacttt ttgtcgattt tttaacgacg cgttacacgg ataatttttt
     2341 ctttgcgtat aatatcgtca ttattgcgcc tttcttgtat ctttaccgca gcgtatatga
     2401 attagaaaca cgcggtgtta tgtacccgat tattatgttt atcggtgtat tgtcttattt
     2461 tatttttcct gttcaatcgg tcagcgacga aatatttttt attcaacttt taggcgcaat
     2521 tttcactctt gtttttttct actgtgcgta tctttttctc cgatcagaaa aaaagcccaa
     2581 agccgaacaa gcagttcgaa aaaaatgggc ggcgcgtatt tttaatattt cattaatctc
     2641 ttctgttgcg ttaccaccgg cgttcttttt tacgctttat tattttccag tgcggttgga
     2701 catcaactat aacgcacttt ttttcctgcc ggcatttttt tcaataatct ttttaacgct
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacgctaa gtttgcgttg
     2821 ggggctcgtc gcatttcatg tgccgattag tttatgggcg gtgaggcttc tttattttgc
     2881 tttttttgga ttcctttatt ggtttacgat tggctttagc ttgggtgaga tttttcagaa
     2941 tgagaccatt cgattggggc actttattgt attgggagtg tttcttatac tcatcgatcc
     3001 tctgcgtacc gcgcttttga cttcactcga tacgtacacc catgcccgcc ggcaaagcct
     3061 cgacgcgttc ctgtcgcaaa gctttcaaca aatatcaaac ccgcaacggg tgaatcaaat
     3121 cgtcgatagg attgccacgc tcttgagcga aggtttgagc gcgtcgtggg ccaaagttgt
     3181 cgtgagtaac gatttgtttc gggattgggt tgtcagtagc gataaagtgg tggcactacc
     3241 ttccgatgat ccgttttgga ttgaagcgta tcgtcctttg cgtaaaggac gatttttagt
     3301 tttaacacgg acttttatcg gtccagtgcg cgattttttg caaaagcagg gcggctatct
     3361 cgttataacg atgagtaaat ttaaggcagg tatactcatt gccgaacgcc gcgaaaatac
     3421 t
//
LOCUS       DAOMPD010000289         3406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11365_length_3406_cov_2.911967, whole genome shotgun sequence.
ACCESSION   DAOMPD010000289 DAOMPD010000000
VERSION     DAOMPD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11365_length_3406_cov_2.911967"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1434
                     /locus_tag="PLY93_09960"
     CDS             <1..>1434
                     /locus_tag="PLY93_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP69842.1"
                     /translation="MTDVESMEVLKGPGSALYGRNEPGGAINITTKKPLDHAHASVEG
                     MVGFWNMHRLAADVGTPFIDNQLLTRMNVGYYKTDGYRGLSREIIEVLPTVTWKFTEK
                     HALTLDYDFRNIKTRPDNYGILFDKNNNKLSAGSPSRTYYSPFNTAEQTINRISALYE
                     GKFNEHLKLRSTLIYDDRSIFMVRNANVTVNTNDTVTNRDSRVQNDKIQTVLHQDEAV
                     LKFNTGSWAHTVLAGFEFEYAKMNTARDSYKPLAAIGLSNPYPTESDYKSLPKTQNYD
                     RLVELITTSAYAQEQVAFSKYVKARAGVRLDHVDASDKGIGTQPGNNTVQQRTISMAR
                     DLISYQAGLVYQPWEISSFYGGYSEGRYIAANTEAPYLYTEPETSSQLEFGNKLSLFK
                     DKLQTNLAIFRTTRASYAFTGTNGNVNFLGGKRRTDGIELDVSGSPIAGLWIIFNSAL
                     MDSKVMTESTGTGVNAVDLAGKTPQNIP"
     assembly_gap    1435..1444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1445..1737
                     /locus_tag="PLY93_09965"
     CDS             <1445..1737
                     /locus_tag="PLY93_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP69843.1"
                     /translation="AGSLWTTYQIKNGFFKGLGFGGGPTYKSAVYVDIKNIMQVPGYV
                     VADAVLFYKKEKWMAQINFRNITNTVYYTSPSFAGAMPGEPFNVVISAKASL"
     gene            1795..2469
                     /locus_tag="PLY93_09970"
     CDS             1795..2469
                     /locus_tag="PLY93_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69844.1"
                     /translation="MKRIYLYMVVLFMLPMAIHAHYIWIEYDKGREAKVYFGGVEDNE
                     REVLPGKLDKIIGLKAHTIDAQGMLKESVVKKTSHYLGVEAQPNTTSIYVETLVMPVA
                     DLSKFGLGVVKPNYYARFGMHRTSSPQKAEMNLDILPVANAKDTMQILFRGKGLEKAK
                     VSLYAPNTWMKEYRTDKDGKIQLSLPWTGEYVLEVIYTEKVSGKFDGKAYEAIRHRAT
                     YTFVVE"
     gene            2466..3170
                     /locus_tag="PLY93_09975"
     CDS             2466..3170
                     /locus_tag="PLY93_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRP69845.1"
                     /translation="MKTNILHRRIASFSAVFLVISAVTGLVWAYAPHIFFKDGYLKKK
                     QYVKAPSFTTIKATEKEVIQKAGLFFHSYDKIVSITLKPEASKLVYLVVKQDDKKTQS
                     LLFDALSGKMLSPIDEKFAIEIAAQYVPKGSVFKLSDAIDIYTHRKGRKVNNVYRVVF
                     TGSETIEIFIDRHSGEIIEESSPSRRFLFLMQKLHQLEFFGTKKELTAIPGISLLILA
                     ITGVMILWRRYTRNRE"
     gene            complement(3187..>3406)
                     /locus_tag="PLY93_09980"
     CDS             complement(3187..>3406)
                     /locus_tag="PLY93_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69846.1"
                     /translation="RITLSALIRLALELFMNRIALERQSYRFVTDEQLKWQAIRFVEK
                     YTSYAQNIASRPHTHRFQWLNFATTTYW"
BASE COUNT          988 a          734 c          791 g          883 t
ORIGIN      
        1 atgaccgatg tagaatcgat ggaagtctta aaagggcctg gctcagcgct ctatgggcgt
       61 aacgaacctg gcggcgcaat caatatcacc accaaaaaac cacttgacca tgcgcatgcc
      121 tcggtcgaag ggatggtggg cttttggaac atgcaccgtc ttgccgccga tgtcggaact
      181 ccctttatcg acaatcaatt actcacgcgc atgaacgtgg gctattataa aactgacggc
      241 taccgagggc tctcgcgtga aattatcgaa gtgctgccga cggtgacgtg gaagtttacc
      301 gaaaagcatg cactcacttt agactacgat ttccgcaaca ttaaaacgcg cccagataac
      361 tacggtattt tattcgacaa gaataataat aaattgagtg cggggagtcc atcgcgcacg
      421 tattattctc ctttcaacac cgcagagcaa accatcaacc gcatctctgc gctttatgaa
      481 ggtaaattta acgagcattt gaagttgcgt tccactctaa tttatgacga tcgctcgatt
      541 tttatggtgc gtaacgcaaa tgttaccgtc aacacgaatg acactgtgac gaatcgtgat
      601 tcacgagtgc aaaacgataa aatccaaacc gtattgcacc aagatgaagc agtgctcaaa
      661 ttcaatacgg gttcttgggc gcatactgtt ctcgcaggat ttgaatttga gtatgccaaa
      721 atgaatactg cacgcgattc gtataaacct ttagcggcaa tcgggctttc aaacccatac
      781 ccgacagaaa gcgattataa aagtctgcca aaaacacaaa attatgaccg cttggttgag
      841 ctcatcacca caagcgcgta cgcacaagaa caagttgcgt tttcaaaata cgtgaaagcg
      901 cgtgcgggtg tgcgcttaga ccacgttgac gcgtccgaca aaggaatcgg gactcaaccg
      961 ggaaataata ccgtacaaca acgcaccatc agcatggcgc gtgatcttat cagttaccaa
     1021 gcgggactcg tgtatcaacc ttgggaaatt tcttcttttt atggtggtta ctccgaaggg
     1081 cgttatatcg ccgccaatac cgaggctccg tatctttata cagagcctga aacgtcgtcg
     1141 cagcttgaat ttggaaacaa actttcttta tttaaagaca agcttcaaac caatttagca
     1201 atttttagaa ccactcgcgc gagttacgct ttcaccggca ccaacggcaa tgtcaatttc
     1261 ctgggtggta aaaggcgcac cgatggtatc gaactcgatg tttcaggtag tcccatcgcc
     1321 ggcctttgga ttatttttaa ctcggcgttg atggattcta aagtgatgac agaatcaacg
     1381 ggcacgggag taaatgctgt cgatcttgca ggtaaaaccc ctcaaaatat tcccnnnnnn
     1441 nnnnacgccg gttcgctctg gacgacgtac caaatcaaga atggtttttt caaaggttta
     1501 ggcttcggcg gtggccccac gtataaatcg gcggtgtacg tggatattaa aaatatcatg
     1561 caagtacctg gttacgttgt cgctgatgca gtgcttttct ataaaaagga aaagtggatg
     1621 gcacaaatca acttccgtaa catcaccaac acagtgtatt atacgtcgcc gtcttttgcg
     1681 ggagcgatgc cgggcgaacc cttcaacgtg gtcatttcgg caaaagcgtc actatagaac
     1741 ataaacttgc cgcagcttgc tgcggcaagt ttatactgat ttaaggagtt attgatgaag
     1801 agaatatatt tgtatatggt ggtgttattc atgctgccga tggccattca cgcgcactat
     1861 atatggattg agtacgataa aggccgcgaa gcaaaagttt atttcggtgg cgtggaagac
     1921 aacgagcgcg aagttttgcc ggggaagctc gacaaaataa tcgggctcaa agcgcacacg
     1981 atagatgcac aaggtatgct gaaagaaagt gtagtcaaaa agacatccca ctatttgggg
     2041 gttgaagctc agccgaatac aacgagtata tatgtagaaa cgttagtgat gcccgtggcg
     2101 gacttgagca agtttggctt gggggttgtg aaaccgaatt actacgcccg ttttggcatg
     2161 catcgtacaa gctcgccaca aaaagcggag atgaatttag atattttgcc cgttgcaaac
     2221 gcgaaagaca ccatgcaaat tttgtttcgt ggtaaagggc tcgaaaaagc aaaagtttct
     2281 ctttatgccc ccaatacgtg gatgaaagag taccgtacgg acaaagatgg taaaattcaa
     2341 ctatcgctgc cgtggacggg tgagtatgtg ctcgaagtta tctacaccga aaaagtatcc
     2401 ggaaaatttg acggtaaagc gtatgaagct atacgccacc gcgccacata cacttttgtc
     2461 gtagaatgaa aacaaatata ctgcaccgcc ggattgcttc tttttcggca gtgtttttgg
     2521 tgattagtgc agtcactggt cttgtgtggg cgtacgcgcc acatattttt ttcaaggatg
     2581 gatacttaaa gaagaagcag tatgttaaag caccgtcatt cactacgata aaagctactg
     2641 aaaaagaagt tattcagaaa gcaggtctgt ttttccatag ctatgataaa atagtgtcta
     2701 taacgctgaa accagaagcg tctaagcttg tttatttagt cgtaaagcaa gatgataaaa
     2761 aaactcaatc actacttttt gatgcgctct ctggaaaaat gctgagcccc atcgatgaaa
     2821 aatttgcgat tgaaattgcc gcgcaatatg tgcctaaagg ttcggtattc aagttgtctg
     2881 atgcaatcga tatctatacg caccgtaaag gtcgcaaagt gaataacgtt taccgcgttg
     2941 tgtttacggg aagtgaaacg atagaaattt ttatcgatcg ccactccggt gaaattatcg
     3001 aagagtcaag cccgtcgcgg cgctttttgt tcctcatgca aaagctacac cagttggaat
     3061 tttttggaac aaaaaaagaa ctgaccgcaa ttcccggaat ttctttgctt atcctcgcca
     3121 ttactggtgt catgatacta tggcgacggt acacacgcaa tcgggaatga gacagactat
     3181 ccgttgttac caataagtgg ttgtcgcaaa attaagccac tggaaacggt gtgtgtgtgg
     3241 gcgggatgct atattttggg cgtaagaggt atatttttct acaaaccgaa tggcctgcca
     3301 tttgagttgt tcgtcagtga caaatctata gctctgtctt tccagagcta tcctattcat
     3361 gaacaattcc aatgcaagcc gaataagggc tgatagcgta attcgc
//
LOCUS       DAOMPD010000290         3400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11401_length_3400_cov_2.538714, whole genome shotgun sequence.
ACCESSION   DAOMPD010000290 DAOMPD010000000
VERSION     DAOMPD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11401_length_3400_cov_2.538714"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLY93_09985"
     CDS             <1..231
                     /locus_tag="PLY93_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69847.1"
                     /translation="LKQFYTSLREQVSLEKKKSIRQKREKIETKEKRTAKEMLDLSLL
                     QFFDGDPTKAFETLEKLTAAMEKQKQKNSALK"
     gene            238..>1073
                     /locus_tag="PLY93_09990"
     CDS             238..>1073
                     /locus_tag="PLY93_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRP69848.1"
                     /translation="MRNSSFSLKRRYYQKSIISLVNIEEAIARVQELIDEIFDDTRAI
                     VLYWNDAAGAFVPVKEEDREAHLKFRIFDDFMLWLGEQNRIFSKHDFEHSKKFVDIRE
                     YALAFFKTTNAQILVPFNLNNSVLAVLYMVAREEGKRFNKRELRFLLEIRDITTVSLS
                     NAALYERLHAMLFHLEEKVKERTSELSNAQAQLIQQEKMASLGVMVAGIAHEINTPTS
                     VVSGAAENLNKNLQLIIAEIVDNRIESEAMRALLREAARVRENKDRKALNATEKFTKV
                     KN"
     assembly_gap    1074..1102
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1103..1872
                     /locus_tag="PLY93_09995"
     CDS             <1103..1872
                     /locus_tag="PLY93_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69849.1"
                     /translation="LISSEKIDEANARQRADFLLDMGLHDDTSIIQIAANSNEKEFQI
                     FRALVNIDKNLNNMAFALSSILNIVKALKHYSHRDQAEAENVDVRDGIENTLTVFHNQ
                     LKHGIHIERHYEEVSKISCNPGELNQIYSNLIVNAVHAMKGEGRLDIFVRERSLDKIF
                     DFKNVLSDEKLEEALGTATQNRYVLISIQDSGSGIPVEYQSKIFDPFFTTKAPGEGTG
                     LGLGIIRNVILKHKGFLALSSKPGRTRFTIALPCAAS"
     gene            complement(1869..2333)
                     /locus_tag="PLY93_10000"
     CDS             complement(1869..2333)
                     /locus_tag="PLY93_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69850.1"
                     /translation="MRFVLLFPLFFLTIGCGLFKTHGEFSFAVADQSMLPVLEQHFFY
                     PKRFIKPQTTPIFESDDVLWYAYRPHNIDATAFYGISLQKKTLGFQEIDLRNRIIPSG
                     QNLIIDKYERLEEGEYRLRVAYQNKVIDQIDFIIVADSATESIDYEKDESEI"
     gene            complement(2350..3294)
                     /locus_tag="PLY93_10005"
     CDS             complement(2350..3294)
                     /locus_tag="PLY93_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69851.1"
                     /translation="MRRPLALLIALTAVITYCKKNPHISQKEIEKLTTLKVDKILIQK
                     KYQLDESASLEDVTFFQAAQKTYAIVVTPTEKSYTLIRQVDFGILLQDIKLHFISIGN
                     KKSQALVTHGLAEKRIVLFDAQNFMQEFTPQSVDTKAVKIIKVEEKPFDELLVFGDKN
                     FRFNGIRWIPFDPDEIFPYLENFTAAGDDSFLEITNRGQFGTRVIVSIAFTDVTGDIS
                     QKLKLTKDIPTVRLYKPGYPAHKKGGGVVALRHPLIEIQKDSWGKNGRIKLPLFMKDI
                     QHFTLRAVYSQRGHSLDWPSTVSEGIVKDGQGYLAVER"
BASE COUNT          985 a          673 c          789 g          924 t
ORIGIN      
        1 ctaaaacagt tttatacttc gttgcgcgaa caagtctctc ttgaaaaaaa gaagtctatc
       61 cgacaaaaaa gagaaaaaat tgagacgaaa gaaaagcgca ccgccaaaga aatgctcgat
      121 ttatcgcttt tgcaattttt tgatggcgac ccaactaaag cttttgagac gttggaaaaa
      181 ctgaccgctg caatggaaaa gcaaaaacaa aaaaatagcg cattgaagta aagcacgatg
      241 cgtaatagtt cattttcgtt aaaacggcgg tactaccaaa aaagtattat ttctttagtg
      301 aatatcgaag aggcgattgc gcgcgtgcaa gaactcatcg atgaaatttt cgacgatacg
      361 cgcgctatcg ttctttattg gaacgatgcc gcaggtgcat ttgttccagt caaagaagaa
      421 gaccgtgaag cgcatctcaa attccgcatc ttcgacgact ttatgctttg gttgggtgaa
      481 caaaatcgaa tttttagcaa gcacgatttc gagcattcca aaaaatttgt cgacatccga
      541 gaatatgcat tggctttttt caaaacaacc aacgcacaaa ttttagtacc ttttaattta
      601 aataattcag tgctcgccgt tttatatatg gtggcgcgcg aagaagggaa acgctttaac
      661 aaaagagaac tgcgttttct tttagaaatt cgcgatatta ctactgtttc gctttccaac
      721 gccgcgctct acgaaaggtt gcatgcgatg cttttccatt tggaagaaaa agtgaaagag
      781 cgcacgagcg aactttctaa cgcgcaggcg caactcattc aacaagaaaa gatggcgagt
      841 ttaggcgtta tggtggcggg gattgcgcat gagatcaaca cccccacatc ggtggtgagc
      901 ggcgctgctg aaaacttaaa caaaaacctg caactcatca ttgctgaaat tgtggacaac
      961 cgcattgaat ccgaagcgat gcgcgcactt ttacgcgaag cggcgcgggt gcgcgaaaac
     1021 aaagacagaa aggctctcaa cgccaccgaa aaatttacta aagtaaaaaa tttnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnagcttatt tcttccgaga aaatagacga agcgaacgca
     1141 cgacaacgcg ccgattttct cctcgatatg ggtttgcacg acgatacaag catcattcaa
     1201 atagcggcaa attctaacga aaaagaattt caaatttttc gcgcgctcgt caatatcgat
     1261 aagaacttaa ataacatggc gtttgcgctc tcgtcgatac tcaatatcgt gaaagcgtta
     1321 aagcactatt cacaccgcga ccaagcggaa gcggagaatg tcgacgtgcg cgatggtatc
     1381 gaaaatacat tgacggtatt tcataatcaa ctcaagcacg gaattcatat tgagcggcac
     1441 tacgaagaag tgtcaaaaat ttcatgtaac cctggcgaac tcaatcaaat ttattcaaac
     1501 ttgattgtga acgccgtgca cgcaatgaaa ggcgaggggc ggttagatat tttcgtacgc
     1561 gagcgttcgt tggataaaat tttcgatttt aaaaatgttt tgtccgatga aaagttagaa
     1621 gaagcattgg ggacggctac tcaaaaccgc tatgtattga tttcgataca agattcgggg
     1681 agtggaatcc ctgttgagta tcaatccaaa atttttgatc cgttttttac aaccaaagcc
     1741 ccgggcgaag gcacagggct tggtttgggc atcatccgca acgtgatatt aaagcataag
     1801 ggatttttag cattgtcgag taaaccgggt agaactcgct ttacgattgc ccttccttgt
     1861 gccgcgagtt aaatttcact ttcgtctttt tcgtagtcga tcgattcggt ggcggaatcg
     1921 gcgacgataa taaaatcgat ttgatcgatc actttatttt ggtacgctac ccgcagtctg
     1981 tactcgcctt cttcgagccg ttcgtattta tcgataatca aattttgacc gctggggata
     2041 attcggttgc gtaagtcgat ttcctgaaag cctaaagttt ttttctgtaa tgaaatgccg
     2101 taaaacgccg tcgcgtcgat attgtggggg cggtatgcat accagagtac gtcgtcgctt
     2161 tcaaaaatag gcgttgtttg aggtttaata aaacgcttcg gatagaaaaa gtgttgctcg
     2221 aggacaggga gcatactttg atcggcaacg gcaaatgaaa attcaccgtg tgttttgaaa
     2281 agaccacagc cgatagttaa aaaaaataac gggaacaata gtacgaaccg catactatta
     2341 ttttcggcgt tatctttcga cggctaaata gccttgtccg tcttttacaa tgccttcgct
     2401 gacggtcgac ggccaatcta aactatgccc gcgttgtgaa tacacggcgc gtaatgtaaa
     2461 atgttggatg tctttcataa aaagcggcag cttgatgcgg ccattttttc cccacgagtc
     2521 tttttggatt tcaatgagcg ggtgccgtaa agcgacgaca ccaccacctt ttttgtgcgc
     2581 gggatatcct ggcttgtaaa gacgcaccgt gggaatatct ttagtcagtt ttaatttttg
     2641 actaatgtca ccggttacat cggtaaacgc aatggagacg ataacacggg tgccaaactg
     2701 gcctcggttg gttatctcga ggaagctgtc atctccagcg gcagtaaaat tttccaagta
     2761 ggggaaaatt tcatcgggat caaagggaat ccaacgaata ccgttgaagc ggaaattctt
     2821 atcgccaaaa actaaaagtt cgtcgaaagg cttttcttca actttgataa ttttaaccgc
     2881 tttggtgtcg acgctttgtg gagtgaactc ctgcataaaa ttttgggcgt caaaaaggac
     2941 gatgcgcttt tcggctaacc catgagtgac aagcgcttgc gattttttat tgccgatgct
     3001 aatgaagtgg agtttgatgt cttgcagaag aataccaaaa tcgacttggc ggatgagcgt
     3061 gtagcttttt tcagtgggag ttacaacgat ggcatacgtt ttttgtgccg cttggaagaa
     3121 agtcacatct tctaaagagg cgctttcgtc gagttggtat ttcttttgaa tgagtatttt
     3181 atcgacttta agtgtagtga gcttttcgat ttctttctgt gaaatgtggg gatttttttt
     3241 acaataggta ataaccgctg tgagcgctat caaaagagca agggggcgac gcatagagtc
     3301 agtgttcgag aggggaacag gcggaaaagt ggttaggagc ctgtgcgtat gcaacccaca
     3361 gagataactg acacttttgg ggtcaagttg agtgcgtttt
//
LOCUS       DAOMPD010000291         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11407_length_3398_cov_2.380796, whole genome shotgun sequence.
ACCESSION   DAOMPD010000291 DAOMPD010000000
VERSION     DAOMPD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11407_length_3398_cov_2.380796"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PLY93_10010"
     CDS             complement(<1..1650)
                     /locus_tag="PLY93_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRP69852.1"
                     /translation="MAQATKDKVYDFVIIGSGFGGSVSAYRLSQKGYKVLVIEKGKWF
                     KNAEDFPKSNWNLWKWLWLPTLKMFGLFKMTIFRHVTIFSGVGVGGGSLVYANTLPVP
                     KTPFFKSKSWAHLANWEKELKPHYQTALKMVGAARNPRLFDGDNVLKEIAISHGHKKA
                     FEHTNVAVYFGKEGETVSDPYFKGEGPTRTGCTFCGGCMLGCHYNAKNTLDKNYLYLA
                     QKLGCEIIAEREVVDVQYHENNTHPYGIIFHRSTAYFKKKETVRAAGVVFAGGVLGTN
                     ALLLKLKEKKSLPNISDQLGFGIRTNSESLLGVTTERRDIDFSKGIAIGSILDVDKDT
                     HLEVVRYPEGSGFFRTAMVPAVMGSNIFIRLGKMFWMYLCHPIKYLRAFFVPDWAKYT
                     QIMLFMQSLDSTLRLKRGLFRLKTALDKGPAPTSSIPMAPKLAEEFGKKTKGTVHSLS
                     IEVLTGLATTAHILGGACMGSTPQEGVIDSQNRLFGYDNMYVCDGSMISANIGVNPSL
                     TIMALTERAMSFVPMKGKAVSGTRKPVAEKKAAKKKVAVKKK"
     gene            complement(1776..3224)
                     /locus_tag="PLY93_10015"
     CDS             complement(1776..3224)
                     /locus_tag="PLY93_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRP69853.1"
                     /translation="MRLPDKPLSQQAIFKELSQLEQLDPHYKKAKLFSLVYYLGEAYT
                     GFLSKVFYKFFSANGLNPGAFKSLKVMERNIVAMTADLFHGDDAVCGVVTSGGTESCL
                     LAVKTYRDFARVTKRIKKPEMIVADTAHVAWLKGAQYFNVKIHRVHSLPEGGMDIDAV
                     KKKINRNTILLLGSAPEYPHGLIDPINKLSALAVKYKIPLHVDACVGGFILPFIEENG
                     HALPPWDFRVEGVTSISADIHKYGFAAKGASLILYRHQRHFEHQVFVDVDWPGGIFAS
                     PALLGTRPGGAYAAAYAALLANGRKGYLKLAKDALSATEQLKKGITELGFKILGNPQA
                     TLFAYRSSRPDIDVFAVADLMEKHGWFIDRIQKPDALHAMVTPGHLRIVKDYLRDLKA
                     CLIIAQKQPELSQKGQAATYGMMAHVPFRKIVKKQVLKMFASTYRISGGVLNLEDVSL
                     ENSVSDAGTPVKRPLAERFALWLAERNRKRNR"
BASE COUNT          856 a          846 c          809 g          887 t
ORIGIN      
        1 cttttttttc actgctactt ttttctttgc ggctttcttt tcagctacgg gcttgcgtgt
       61 gccactcact gccttgcctt tcatcggcac aaacgacatc gcgcgctcag tgagagccat
      121 gattgtgagc gagggattaa cgccaatgtt cgcgctaatc atcgagccgt cgcaaacgta
      181 catattgtcg tagccaaaaa gtcggttttg gctatcgatc acgccttctt gaggggtcga
      241 ccccatgcac gcaccaccca aaatgtgcgc agtggtcgca aggccggtca acacttcaat
      301 cgagagcgaa tgcactgtac cttttgtttt tttgccaaac tcttcggcaa gcttgggcgc
      361 catcgggatg gaagacgtgg gcgctgggcc tttgtcgagc gcggttttaa gccgaaaaag
      421 accgcgcttc agacgcagcg tcgaatcgag gctttgcata aagagcataa tctgtgtgta
      481 cttcgcccag tcaggaacaa aaaatgcgcg caagtattta atcggatggc agagatacat
      541 ccagaacatt ttacccaagc ggataaaaat gttcgagccc atcaccgctg gcaccatcgc
      601 tgtgcggaaa aatcccgaac cttcagggta gcgcaccact tcgaggtgcg tgtctttatc
      661 gacgtcgaga atcgaaccga tggcgatgcc ttttgaaaaa tcaatatcgc gccgttcggt
      721 ggtgactccc aacaaacttt ccgaattggt acgaatacca aagcctaatt ggtccgatat
      781 attcggtaac gatttttttt ctttgagttt gagcaagagt gcgttcgtgc ctaaaacacc
      841 gccggcaaac acaacgcccg cagcgcgtac tgtttccttc tttttgaaat acgcggtaga
      901 acgatgaaaa ataattccat aagggtgcgt attgttttcg tggtattgca catccaccac
      961 ttcgcgttcg gcaataattt cacaacccaa tttttgagcg aggtagagat aatttttatc
     1021 gagagtattt ttagcgttgt aatggcagcc gagcatacag ccaccgcaaa acgtacagcc
     1081 cgtgcgcgtg gggccttcgc ctttgaaata gggatcgctc accgtctcgc cttctttgcc
     1141 gaaataaacg gcgacgttcg tgtgttcgaa agcttttttg tggccgtgcg aaatggcaat
     1201 ttctttgagg acattgtcgc cgtcgaaaag ccgtgggttg cgcgctgccc ccaccatctt
     1261 gagcgcagtt tgatagtgcg gttttaattc cttttcccag ttggcaaggt gcgcccagct
     1321 tttcgatttg aaaaacggcg tcttgggcac gggcagcgtg ttggcgtaca cgagcgaacc
     1381 accgcccaca cccacaccag agaagatagt gacgtggcgg aaaatagtca tcttaaaaag
     1441 gccaaacatt ttgagagtgg gcagccacaa ccatttccat aaattccagt tactttttgg
     1501 aaaatcctcc gcgtttttga accacttccc tttttcgata acgagaactt tatatccctt
     1561 ttgcgagaga cggtatgccg aaactgagcc gccaaacccg gagccgatga tgacaaaatc
     1621 gtatacttta tcctttgttg cctgcgccat acgccaccga gaaaaaaaga ctctatccgc
     1681 caactctaat gtggttagga gcctttgagc caactcgctg agacaatttt ttttatacgc
     1741 tgcgcgagtg ggcgcacagg ctcttaggca aactcctaac gattccgctt gcggttgcgt
     1801 tcggcgagcc acaatgcgaa acgctccgcc aaaggtcgct ttaccggcgt gccagcatct
     1861 gacacagaat tttctaaaga aacgtcttct aaatttaaca cgccgcccga aattctgtac
     1921 gttgatgcaa acattttcaa gacttgtttt ttgactattt ttctaaaagg cacgtgcgcc
     1981 atcatgccat acgttgccgc ttgtcctttt tgagagagct ccggttgctt ttgagcgatg
     2041 attaagcacg cttttagatc gcgtaaatag tctttcacaa tgcgtaagtg ccctggggtc
     2101 accatcgcgt gcagcgcgtc gggcttttga attctatcga taaaccatcc gtgcttttcc
     2161 atgagatcgg caaccgcaaa cacatcgatg tcaggccggc tcgaacggta tgcaaagaga
     2221 gtcgcttgcg gattacccag aatcttaaaa cctaattctg taattccttt tttgagctgt
     2281 tcggtggcgc tgagtgcatc tttcgcgagt ttcaaatatc ctttgcgccc attggccaac
     2341 aacgccgcgt acgccgccgc gtatgcgccg cccgggcgtg ttcccaaaag cgcaggtgag
     2401 gcaaatatcc cccccggcca atcgacatcg acaaacacct gatgttcaaa atggcgctga
     2461 tggcggtata aaatgagcga cgcgcctttc gctgcaaaac catatttgtg gatatccgcc
     2521 gaaatggaag taacgccttc gactctaaaa tcccaaggcg gcagagcgtg gccattttct
     2581 tcgataaatg ggagaataaa cccaccgaca cacgcgtcga cgtgcaatgg aattttatat
     2641 tttactgcaa gcgcagataa cttatttata gggtcgatta acccatgcgg gtattcgggc
     2701 gcgctaccta aaagcagaat ggtgttgcgg ttgattttct tttttaccgc atcgatgtcc
     2761 atgccgccct cgggtaaact gtgcacacgg tggattttta cgttgaagta ttgcgcgcct
     2821 ttaagccatg ccacgtgcgc ggtatcggca acgatcattt caggcttttt gattcgtttt
     2881 gttacacggg caaaatcacg gtatgttttt accgccaaaa gacaactctc agtaccaccc
     2941 gaggtcacta cgccgcagac ggcatcgtcg ccgtggaata aatcggcagt cattgcgaca
     3001 atgttgcgtt ccattacttt aaggcttttg aaagctcccg gatttaatcc gttggctgaa
     3061 aaaaacttgt aaaaaacttt gctcaagaaa ccggtgtacg cttcgcctaa gtaataaacc
     3121 aaactgaata actttgcttt tttgtagtgg ggatcgagct gttcgagctg acttagttct
     3181 ttgaagattg cttgctggga taggggttta tcgggtagac gcatttattt tacctcaaca
     3241 aataatataa tccaacacct aaataattac caattccata accaatgagg ccggtagtaa
     3301 ggccaatggg gatgagatcg ctgcgatcga tagcgctggc ggcgggcggc acaaaaggag
     3361 gcgagcaaat agcagaaaca gagaccacaa taaaagta
//
LOCUS       DAOMPD010000292         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11438_length_3388_cov_2.405941, whole genome shotgun sequence.
ACCESSION   DAOMPD010000292 DAOMPD010000000
VERSION     DAOMPD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11438_length_3388_cov_2.405941"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="PLY93_10020"
     CDS             complement(<1..1544)
                     /locus_tag="PLY93_10020"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRP69854.1"
                     /translation="MAQKYVYFFSAQKTEGGAGDKKLLGGKGANLAEMTTLGIPVPPG
                     FTLSTEVCTYFYANERKYPAELQAQIESGVTELETTIGKKFGDTKNPLLVSVRSGAPV
                     SMPGMMDTILNLGLNDATVLGLIASTGNERFGYDSYRRFIQMYGNVVMGMKHHDFEHI
                     LDELKKSKGVTDDLALTAQDLQKLITLYKAEVKKITGKDFPQNPKEQLMGAIDAVFAS
                     WMNDRAITYRKLNNISESLGTAVNVQSMVFGNMGDDCGTGVAFTRNPANGERKFFGEF
                     LMNAQGEDVVAGIRTPLSIEKLGEKMPEALKELLSYADKLERHYKDMQDIEFTIERSK
                     LYMLQTRNGKRTAFAAVRTAVEMVDEKLIDDKTALKRIDAHTLPSLLAKIFDEKEKKD
                     VLAKGLVVATGLPAGPGAASGRIALTAEKAVEWAEKGIKSILVRVETSPEDISGMHSS
                     EGILTARGGMTSHAAVVARGMNKPCIVGCSALEVNYNKGTLTFHAGTPQEVALKEGDE
                     ISIDGFT"
     gene            1635..>2847
                     /locus_tag="PLY93_10025"
     CDS             1635..>2847
                     /locus_tag="PLY93_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69855.1"
                     /translation="MSVSSTTPKNYPTLSLRLTLRLGLVMLVVGLLFAAIYFVLSISL
                     FSRLSNEQMLQKKQSAQIIIESTLNSKENINSRTLIREIQEAGLSNDIGVFDMKQQLI
                     DHSNDKIAAALGRYATDPKLRLADVETQRKILAAALRHAKKKSSSLFLVVDNLSYVAY
                     PSKSYTVLVGFRPQSLNYLTLGVSFFLLCLIAVAINTLFVYQYGHSLSTTLMRIVRGL
                     EIDSLHSGGFSPQYAEVASLLQAVEEKTSASSGQTFSSSNPTMAEKSGRTEETQHLLQ
                     NKLFERPFPRMQRYELAVYPRRPKVNTKEFICGAHNDGRIDVLIGITDADSVGALVAK
                     NRIQERFWALSEENTAPTAMAKSLWQAFFASSEYVPGLFFARLDEAEKNLDIYRAGAV
                     HLFEVSGNNCRT"
     assembly_gap    2848..2857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2858..3121
                     /locus_tag="PLY93_10030"
     CDS             <2858..3121
                     /locus_tag="PLY93_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69856.1"
                     /translation="SAQFGSSFTALSQHTLAEGSQLVMVSHDTFSAMELSTDDFADIL
                     AKLSEGKSGKLLLAAILEKIHQKLDAGETVPGLLAVLSEKKKL"
     gene            complement(3193..>3388)
                     /locus_tag="PLY93_10035"
     CDS             complement(3193..>3388)
                     /locus_tag="PLY93_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP69857.1"
                     /translation="FIDTDMTDMIKKREAILAKMLEHIPQKRLGKPEDLAGAAVFLAS
                     DAASYITGQTLVVDGGLTAM"
BASE COUNT          785 a          867 c          841 g          885 t
ORIGIN      
        1 gtgaaaccgt cgatagagat ttcatcgcct tctttgagtg caacttcttg tggcgtgcct
       61 gcatggaaag tgagcgtgcc tttattgtaa ttcacttcga gcgcgctgca acccacgata
      121 cagggtttat tcataccccg cgcgaccaca gcggcatggg aggtcatccc accgcgtgcg
      181 gttaaaatgc cttcggagct atgcatgcct gaaatgtctt cgggggatgt ttccacacgc
      241 actaaaatac ttttgatgcc tttctcagcc cattcaaccg ctttttctgc ggtgagcgca
      301 atgcgaccgc tggcagcacc cggccctgcg ggaagaccgg tggcgaccac caagcctttt
      361 gcgagcacgt ctttcttttc tttttcgtcg aaaatttttg caaggagcga aggcagcgtg
      421 tgcgcgtcga tgcgcttcaa cgcggtttta tcgtcgatga gcttttcatc gaccatttcg
      481 acggcggtac gcacagcggc aaatgcggtg cgcttgccgt tgcgggtttg tagcatgtag
      541 agtttagagc gttcgatggt gaactcgatg tcttgcatgt ctttgtagtg gcgctcgagt
      601 ttgtccgcgt acgatagcag ctctttcaac gcttccggca ttttctcgcc cagcttttca
      661 atcgacaacg gtgtgcgaat gccggcaacc acgtcttcgc cctgcgcgtt catgaggaac
      721 tcgccaaaaa atttacgttc gccgttggcg gggttacggg tgaacgccac accggtgcca
      781 caatcgtcac ccatgttgcc gaacaccatc gactgcacgt tcaccgcagt gccgagcgac
      841 tcagaaatgt tgttgagttt gcggtaggtg attgcgcggt cgttcatcca cgaagcaaag
      901 accgcatcga tggcgcccat gagctgttct ttggggtttt gcggaaaatc tttgccggtg
      961 atttttttca cttctgcttt gtagagggta atcagttttt gtaaatcctg cgcggtgagc
     1021 gctaagtcgt cggtgacacc ctttgacttc ttgagttcgt ccaaaatatg ttcaaagtcg
     1081 tggtgtttca tccccatgac cacgttgccg tacatttgga taaagcggcg gtagctgtcg
     1141 taaccaaagc gctcgtttcc ggtgctcgca atgagtccca acacggtagc gtcgttcaag
     1201 cctaaattca agatggtatc catcatgccg ggcatcgaaa cgggcgcgcc cgaacgcacg
     1261 gatactaaaa gagggttttt ggtatcgcca aattttttac cgatggtcgt ttcaagctcg
     1321 gtgacgcctg actcaatttg ggcttgtaac tcggcagggt atttgcgttc gtttgcataa
     1381 aaatacgtac aaacctcggt ggaaagggta aatccggggg gcaccggtat gcctaaagta
     1441 gtcatttcgg ctaaatttgc gccttttcca ccgagcagtt ttttatcgcc ggcgccacct
     1501 tcagtcttct gtgccgagaa aaaatagaca tacttttgtg ccattacgca gcctcgaaga
     1561 ttttgacgcg gtgtaaagat aaacagaaca tgtgttcaaa tttgacgcgc tatttgccga
     1621 tgctaaagat acgtatgtct gtctcgtcca caacccccaa aaactacccc acgttgtccc
     1681 tgcgcttgac gttgcgctta gggctcgtta tgcttgtcgt ggggctcctc tttgcggcga
     1741 tttattttgt cctgtctatt tccttattct cgcgcctctc gaacgaacag atgttacaaa
     1801 aaaaacaaag cgctcaaatt attatcgaat cgactttaaa ctctaaagag aatatcaact
     1861 cacgcacgct gatacgtgaa atacaagagg ccgggctttc caatgatatt ggcgtgtttg
     1921 atatgaaaca gcaacttatc gaccatagca acgataaaat cgccgcagcg ttaggccgct
     1981 acgccaccga tcctaaactg cgcctggcgg atgtagaaac ccaacgcaaa attttagcag
     2041 ccgcgcttcg acacgcaaag aaaaagtcct cctctctctt tcttgtcgtc gataatctct
     2101 cgtacgtcgc gtaccccagt aaaagttaca ccgtcctcgt tgggtttagg ccgcaaagcc
     2161 tcaattacct cactctcggc gtttcattct ttttactttg cttgatcgcc gttgccatca
     2221 acacgctttt tgtttaccag tacggtcatt cgctttcgac aacactcatg cggattgtgc
     2281 gcggtcttga aatcgattcg ctgcactcag ggggattttc accccaatac gctgaagtcg
     2341 cctcgctctt acaggccgtg gaagaaaaaa caagcgcctc tagtggacaa acgttttcga
     2401 gtagcaatcc cactatggct gaaaaaagcg ggcgcacgga agaaacgcaa catttattac
     2461 aaaataaatt attcgagcgc cctttcccgc gcatgcagcg ttacgagttg gctgtctatc
     2521 cacgccgtcc caaagtaaac acgaaggagt ttatctgtgg cgcgcataac gatggccgca
     2581 ttgacgtgct catcggcatc accgacgccg actctgtcgg cgcgctcgtt gccaaaaacc
     2641 gtatccaaga gcggttttgg gcgttatccg aagagaatac ggcaccgaca gcgatggcta
     2701 aaagcctgtg gcaggcgttt tttgcttcga gtgaatacgt acccgggctt ttcttcgcac
     2761 gcctcgacga ggcagaaaaa aatttggata tctaccgcgc aggggctgtg catctttttg
     2821 aggtgtcggg gaataactgc cgtacccnnn nnnnnnnagt gcacagtttg ggagcagctt
     2881 cactgcgctc tcacaacaca cattggcaga aggcagccaa ctggtgatgg tttcacacga
     2941 cacatttagc gcaatggagt tatccaccga cgactttgcc gatatccttg ctaaattgag
     3001 tgaaggcaaa agcggcaaat tgttacttgc agccattttg gaaaaaatcc accaaaaact
     3061 cgatgcggga gagacggtgc caggactttt agcggtgctt tcagaaaaga aaaaactata
     3121 aattcagcat tttgagaata tcggtagctg tctaagagcc gcttgcacgg ccattcaggg
     3181 agcgtccgag tttcacattg cggtcaagcc gccgtcgacc accagcgttt ggccagtgat
     3241 gtagctcgcc gcgtcggatg ctaaaaaaac tgctgcgcct gccaagtctt cgggtttacc
     3301 aaggcgtttt tgcgggatgt gctcgagcat tttagcgagt atcgcttcgc gttttttgat
     3361 catgtcagtc atgtcggtgt cgatgaaa
//
LOCUS       DAOMPD010000293         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11569_length_3358_cov_2.321223, whole genome shotgun sequence.
ACCESSION   DAOMPD010000293 DAOMPD010000000
VERSION     DAOMPD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11569_length_3358_cov_2.321223"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLY93_10040"
     CDS             complement(<1..426)
                     /locus_tag="PLY93_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRP69858.1"
                     /translation="MAALKRQLGFLTAAFTVIGSMIGSGVFRTSNQLYFDQRLDSNII
                     LLLWIVGGLVALAGALSYAELAAAYPEAGGEFAYLNRIFGKLPAFLTGFISLTVGFSA
                     PIAFTSLVIVDLVAQGYAQMTRMEVVYPPFRLQLYAAFIP"
     gene            complement(427..1650)
                     /locus_tag="PLY93_10045"
     CDS             complement(427..1650)
                     /locus_tag="PLY93_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69859.1"
                     /translation="MKKIFLLTVRRRKTGVQTLLLALFCLFFLTCCQSTRVVDETTGR
                     AAGAQKDGVSGYYFDDMARYLGGFDLPRDSNLAQYTTSSEYAEHRTKMAKFWEKVRTQ
                     SITPMSKWRDETLGANGKTVCRSDKPAFYPLCGADIINLYTMCPNASEYIMVALEKAG
                     GIPHPEENSRAYFQGLRNMRSVINNIADRNYFFSAHMKTNIVKNEEIPGIAPVLLAFT
                     SGLGWRIVTMEKIYLSPNGEPIALDDPHLDFKPRGVRIWFRTDADPRLRSLTYIEQYL
                     DRASVAPDYPLTKYLAQKRGAFMLLKAAVYLMHNDYYRAVREFFLTIPDTLVQDDSGF
                     RYVDLRDKWDIEIYGNFTGPAIRMSVRPQNPVQPELAKLYQEKNPKELPFNFGYGSLR
                     RPPRSNLMVARRKEV"
     gene            complement(1659..2777)
                     /gene="prfB"
                     /locus_tag="PLY93_10050"
     CDS             complement(1659..2777)
                     /gene="prfB"
                     /locus_tag="PLY93_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRP69860.1"
                     /translation="MANSLKEIISELSELKETIETRFKELHLEEAEAELSDLRQKMED
                     PTLFADDSKVEKVRELQTRIAYLDKKVSAWKDLILELSDAHEYAEIAAMEKAENLAGE
                     IEKRLRDARTIFDKLDLESLLTGPDDMANAFVNIHPGAGGTESQDWADMLLRAYSRYA
                     EKEGFELEMVDYQEGEEAGIKNATLYIRGPNAFGYLKAENGIHRLVRISPFDSNKRRH
                     TSFASVHVSPEISDEVDVEVLEKDLRVDVYRASGAGGQHVNKTESAVRITHLPTGIVV
                     SSQSQRSQIQNRLTCMKMLKAHLYAKARAEKEADIESRAGERKDISWGNQIRSYVFHP
                     YNMVKDLRTGYETAQIQNVMDGELSPFIDAYLRQATHN"
     gene            complement(2893..>3358)
                     /locus_tag="PLY93_10055"
     CDS             complement(2893..>3358)
                     /locus_tag="PLY93_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69861.1"
                     /translation="EHQSRLVAHPARRGRAFPPDRSAADPAPDRGQPNPPGGGGGCAC
                     TLYIARPPLYLITQGKEKHYAYSDPEKNRILKTFKQDSKYTIQRYKGLGEMNPEQLWE
                     TTMDPANRVMMQVTVDDAVIADDTFVILMGDQVEPRRKFIEENANKVEELDL"
BASE COUNT          781 a          842 c          856 g          879 t
ORIGIN      
        1 ggggataaac gccgcataga gttgcaatcg aaacggcgga tacaccactt ccattcgagt
       61 catttgtgca tacccttggg cgactaaatc gacaatcacg agcgaagtaa aagcaatcgg
      121 cgctgaaaat cccacagtga gcgaaataaa tcccgtcaag aatgcaggca acttaccgaa
      181 gatgcggttg agataagcaa actcgccgcc cgcttctgga tacgccgccg ccaactcggc
      241 gtagctcaac gcgcccgcca acgcgacgag gccacccaca atccacaaaa gtaaaatgat
      301 gttcgagtct aagcgctgat caaaataaag ttggttactg gtacgaaaaa ctccggagcc
      361 aatcatcgaa ccgatgacgg tgaacgcggc ggttaaaaaa cccagctgcc ttttcagcgc
      421 tgccatctat acctctttgc ggcgcgcgac cattaagttc gaacgcggcg gccttcttaa
      481 agagccatag ccaaaattaa acggtagctc tttgggattt ttttcttggt agagttttgc
      541 aagttcgggt tgaacgggat tttgagggcg caccgacatc cgaattgcag ggcctgtgaa
      601 gttaccgtaa atttctatat cccacttgtc gcgcaaatcg acgtagcgaa aacccgaatc
      661 gtcttgcacc aaagtatcgg gtatcgtcaa aaaaaattcg cgcaccgcgc ggtaataatc
      721 gttgtgcatc aaatacaccg ccgctttcag cagcatgaac gcaccgcgct tttgtgctaa
      781 gtattttgta agagggtaat cgggggcaac ggacgcacgg tctaagtatt gttcgatata
      841 cgtgagcgaa cgcagtcgcg gatcggcgtc ggtgcgaaac cagatgcgca caccgcgcgg
      901 tttgaaatct aaatgagggt cgtcgagcgc aatcggttcg ccgttgggcg acaagtaaat
      961 tttttccatc gtgacgatgc gccatcctaa acccgaggta aaagcaagca gcacgggggc
     1021 aatccctggt atctcttcgt ttttgacaat gttggttttc atgtgcgccg agaaaaaata
     1081 atttctgtcg gcaatgttgt ttatcaccga gcgcatattc cgcaaccctt ggaaatacgc
     1141 gcgcgagttt tcttcggggt gtggaatgcc gcccgctttt tcgagagcaa ccatgatata
     1201 ctcactcgca ttagggcaca ttgtgtagag attgatgatg tctgcaccac agagaggata
     1261 aaatgcaggc ttgtccgaac ggcacactgt tttaccgttt gcgccgagcg tttcatcgcg
     1321 ccatttactc atgggggtga tgctttgcgt gcgtactttt tcccaaaact ttgccatttt
     1381 tgttcggtgt tcggcgtatt cggacgaagt ggtgtattgc gctaaattcg agtctctggg
     1441 taaatcaaag ccgcccaagt aacgcgccat atcgtcgaag taatagccgc tcaccccgtc
     1501 tttttgtgcg ccggcagcac ggcctgtcgt ttcatcgaca actcgggtac tttgacaaca
     1561 cgtcagaaaa aaaagacaga agagcgcgag gagcaaagtc tgtacaccgg tttttcggcg
     1621 gcgcaccgtt aataaaaata tttttttcat gggtgtcctt aattgtgggt ggcttggcgt
     1681 aagtacgcgt ctataaaagg agacaactca ccatccatca cattttgaat ttgtgcggtt
     1741 tcataaccgg tgcgtaaatc tttcaccata ttatacggat ggaaaacata actgcggatt
     1801 tgattgcccc acgaaatgtc tttacgctcg cccgcgcgcg attcgatatc ggcttctttt
     1861 tcggcgcgcg cttttgcgta aagatgcgct ttgagcattt tcatacaggt gaggcggttt
     1921 tgaatttggc tgcgctgcga ttgtgacgac accacgatgc cggtgggtaa gtgcgttata
     1981 cgcacagcag attcagtctt gttgacgtgc tgaccgcccg cacccgacgc acgatacaca
     2041 tcgacgcgca aatctttttc caaaacttcg acatcgactt catctgaaat ttcgggcgag
     2101 acatgcaccg atgcaaacga agtatgacga cgcttgttcg agtcaaaggg ggatattctc
     2161 accagacggt ggatgccgtt ttctgctttg agataaccga acgcattggg gccacggata
     2221 tagagcgtgg catttttaat tcccgcctct tcgccttctt gatagtcgac catttccagc
     2281 tcaaagcctt ctttttctgc gtagcgcgaa taggcacgca agagcatgtc cgcccaatct
     2341 tgcgattcgg taccacccgc gccagggtgg atgttcacaa acgcattcgc catgtcgtcg
     2401 ggtccggtga gtaagctctc taaatcgagt ttatcaaaaa tagtgcgtgc atctctgagc
     2461 cgtttttcaa tttctccggc cagattttca gctttttcca tggcggcaat ttcagcgtac
     2521 tcgtgcgcgt cggaaagttc gagtatgaga tccttccaag cgctgacttt tttgtcgagg
     2581 taggcaatcc gcgtttgcaa ttcgcgcact ttttctactt tgctatcgtc ggcaaaaagc
     2641 gtcgggtctt ccatcttttg acgcaaatca ctcagctcgg cttccgcttc ttccaagtgc
     2701 aattctttga agcgagtctc gattgtttct ttgagctcgc ttaactcgct tataatctct
     2761 ttgagagaat ttgccacggg gataagtcgg cgtgtgagat acggcgcaaa gtcttctttt
     2821 gaagttcgat ggaaaagaag gctagcagcc tgtgcgccaa ctcgcggcaa cgcagcgccg
     2881 tttgtcgttt ttttacaaat ctaactcttc gaccttgttg gcattttctt cgatgaactt
     2941 gcgccgtggc tccacttggt cacccatgag aatgacaaac gtgtcgtcgg caatcaccgc
     3001 gtcgtccact gtcacctgca tcatcacccg gtttgcagga tccatcgttg tctcccaaag
     3061 ctgttcggga ttcatctctc ccaatccttt gtagcgttgg atagtgtatt tgctatcttg
     3121 tttgaaagtt ttgagaattc tatttttctc cggatctgag tacgcgtagt gcttttcttt
     3181 gccttgcgtg atgagataaa gcggtgggcg cgcaatgtaa agggtgcagg cgcatccacc
     3241 gccaccgcca ggcggattgg gttggccgcg gtcaggcgca gggtcggctg cggatcggtc
     3301 ggggggaaac gctcgccccc gtcgcgcggg gtgggcaacg agccgggatt ggtgttca
//
LOCUS       DAOMPD010000294         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11576_length_3356_cov_2.383217, whole genome shotgun sequence.
ACCESSION   DAOMPD010000294 DAOMPD010000000
VERSION     DAOMPD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11576_length_3356_cov_2.383217"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLY93_10060"
     CDS             complement(<1..770)
                     /locus_tag="PLY93_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRP69862.1"
                     /translation="MKKKLPQEYLFSLNKEQRYAATHLWERDTQALQVIAGAGSGKTT
                     TLIAAVLSAIDAGYSPERIAVLTFSRRAAHELKERFAKKDTRVGYTGTMHALAWKLLR
                     ASGAQEKILLHSQEIRASILKDIFPEYQTVPIRILLSPHFLSEEKRTLLDERYENYLR
                     TNNAIDFDAMISRATQKSAEENPFDVVFVDEFQDTSPDQVAFIRSLRAQKLFVVGDDW
                     QSIYRFRGADVSMTRDFLQRFENAERAYLVKNYRSGSQ"
     gene            complement(767..1678)
                     /locus_tag="PLY93_10065"
     CDS             complement(767..1678)
                     /locus_tag="PLY93_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HRP69863.1"
                     /translation="MPLESVKNENPLIIHILSNARSLYSTRRFFEEGYKRGHKMKVYP
                     PMQLSAYLERNALELYYTQQKIETPHCIIPRLGQKKPEHTLAIIRHYEMMRVRPLNGS
                     YPMARCRDKFVTMQILAQNKIPVPRSVLIDDPSHIDTTFEKLGNPPYIIKIPTGSQGT
                     GVMIADTKTAARSFIETLLSQDLQLIVQEYIAEARGADYRAIILDGNLIAAMRRQATG
                     DDFRSNIHRGADAAKVELSIEAARTVKLAARILGLTFAGVDLIESNRGPMVLEINASP
                     GIEGIEGATGVNIAEKVIRHLEGLPRA"
     gene            1929..2606
                     /locus_tag="PLY93_10070"
     CDS             1929..2606
                     /locus_tag="PLY93_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HRP69864.1"
                     /translation="MPQNKEYPPPWHLTGAGVIIFFPAHKERVLDSLALGEGNRASFA
                     GGMGAVMLVRYQDAECGPYDELLYIPGFFKHQGRTYLRIAKIFVSTPQSVEWGRRNWA
                     IPKELARFAWQQGKKEWHIDVKETHSEKNIYSISLSPRFFSFPISTALLPWSLLQKQE
                     ERYSDGGDYFLETRLAATGTAKICFIDRVEGSKVFPDYHDVSHGPRIAFAIDPFRMTF
                     PVAKKVR"
     gene            2603..>3356
                     /locus_tag="PLY93_10075"
     CDS             2603..>3356
                     /locus_tag="PLY93_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRP69865.1"
                     /translation="MKHSVYPILGIDIGATTVKFATLNAAGVLGERRALPIASQSNAA
                     FIEQLISIVEDAAFSQCQSVGIGSPGPLDLERGCILSSANMPQIKNLEVIALLKNKFP
                     TKIIRLDNDATCATLGQKFFGVAKTWEDFAVFTLGTGVGGGAFFHNRLERGYKGNFFE
                     VGHIPVDAMRDAGRQCGCGNRGCLEAYASATGIAFSYKEETGVDMSAKDIAARAREGD
                     THAQAAFSLAAHALALGAAAITQTTNITSFIFT"
BASE COUNT          794 a          795 c          886 g          881 t
ORIGIN      
        1 atttggctgc cgctacggta atttttcaca aggtaggcgc gctctgcatt ttcaaagcgc
       61 tgcaaaaagt cgcgcgtcat cgatacatcc gcgccacgaa aacggtaaat actctgccag
      121 tcgtcgccca ccacaaaaag tttttgcgcc ctcagcgaac ggatgaacgc cacttgatcg
      181 ggcgatgtgt cttggaactc gtcgacgaaa acaacatcga atggattttc ctctgcactt
      241 ttttgcgtcg cgcgtgaaat catcgcgtcg aagtcgatgg cgttgtttgt gcgcaaatag
      301 ttttcgtagc gttcatctaa aagagttctt ttttcttcgg ataggaaatg gggagagagc
      361 aaaatgcgta ttggcacagt ttggtattcg ggaaaaatat ctttgagaat tgaagcgcgt
      421 atctcttgcg agtggagtag aattttttcc tgcgcacccg atgcacgcaa gagtttccac
      481 gccaacgcgt gcatcgtgcc ggtgtagccc acgcgcgtat cttttttcgc aaagcgttct
      541 ttgagctcgt gtgccgcacg gcgtgaaaaa gtaagcaccg caatgcgttc tggactatac
      601 ccagcgtcga tggctgacaa aacggcagcg atgagcgtcg tcgttttacc gctacctgca
      661 ccggcaatta cctgcaatgc ttgggtatct ctctcccaaa gatgcgtcgc tgcgtagcgc
      721 tgctctttat tgagcgaaaa gagatattct tgcggaagtt tttttttcaa gcgcgtggca
      781 atccttctaa atggcggatt actttttctg cgatgttgac gccagttgcg ccttcgatcc
      841 cttcgattcc cggcgaagcg ttgatctcta aaaccattgg gccacggttc gattcaataa
      901 ggtcgacacc cgcaaacgtg aggcccaaaa tacgtgcagc caatttgacc gtgcgtgccg
      961 cttcaatcga cagttccact ttggcagcgt cggcgccacg atggatattc gagcggaaat
     1021 cgtcaccggt ggcttgcctg cgcatcgcgg cgatgaggtt tccgtctaaa ataattgcac
     1081 ggtaatccgc gccgcgcgcc tcggcgatat actcttgtac aataagctgc aaatcttggc
     1141 tgagaagagt ctcaataaaa ctacgtgccg ccgttttagt gtcggcaatc atgacaccgg
     1201 tgccttgcga gccagtgggt atttttatga tataaggcgg attgcccagt ttttcaaatg
     1261 tggtgtcgat gtgcgaagga tcgtcgatga gcacactgcg cggcacggga attttattct
     1321 gtgctaaaat ttgcatcgtg acaaatttat cgcggcaacg cgccatggga taactaccgt
     1381 ttaacgggcg cacgcgcatc atttcatagt ggcggataat cgcgagcgtg tgctctggtt
     1441 tcttctgacc caagcggggg atgatgcaat gcggtgtttc aattttttgt tgggtgtaat
     1501 aaagctctaa cgcgttacgc tccaaatacg cagaaagctg catcggtgga tacactttca
     1561 ttttgtgccc acgcttatac ccctcttcaa aaaaacggcg cgtcgagtag aggctacgcg
     1621 cgtttgaaag gatgtggatt attagagggt tttcgttttt tacggattcg agcggcacaa
     1681 gctctgccta atatgtgtat cagcatggaa agaggttttc tggggtgagt ttgcaggctc
     1741 cagcgagttg gcgcacaggc tccatggttg caagttaaac ggagtactaa attctcttta
     1801 accctcgccc aaattatttt aacataactc gatgcgctcc aaattctccc aaaataatcg
     1861 acaaactgcg cgagttattc tatattcgta taaacagctt tgaaagtgag caaaagtgga
     1921 agagaaatat gcctcaaaat aaagaatatc cacccccatg gcatttgacg ggggcggggg
     1981 ttattatttt cttccccgcg cataaagagc gcgtgctcga ctcgctggcg ttgggagagg
     2041 gaaaccgtgc ctcgtttgcg ggcggtatgg gagcggtgat gctcgtgcgt tatcaagacg
     2101 ccgagtgtgg cccttacgac gagctgcttt atattcccgg cttttttaag caccaaggac
     2161 gcacatactt acgcatcgcc aaaatttttg tttccacgcc gcagtcggta gagtggggta
     2221 gacgcaactg ggcaatcccc aaggagttgg cgcggttcgc ttggcagcaa ggaaaaaaag
     2281 aatggcacat cgacgtgaaa gagacgcaca gtgaaaaaaa tatttattcg atttcgctct
     2341 ctccccgttt tttttcattc cccatttcca cggcgctgtt gccgtggagt cttttacaaa
     2401 aacaagaaga aagatactcc gatggcgggg attacttttt agagacgcgc cttgcggcaa
     2461 ccggtacagc aaaaatttgt tttatcgacc gcgttgaggg ctcaaaagtt tttcccgatt
     2521 accacgacgt gtcgcatggt ccgcgcatcg cgtttgcgat tgatcctttc cgaatgactt
     2581 ttcccgttgc taaaaaagta agatgaagca cagtgtgtat cccattttgg gcatcgacat
     2641 tggtgcaacg actgtcaagt ttgcaacgtt aaacgcggcg ggtgttttag gtgaacgccg
     2701 tgcattgccg attgcctcgc aaagcaatgc ggcatttatc gagcagctga tttcaatcgt
     2761 ggaagatgcg gcgtttagcc aatgccaaag cgtcggcatt ggctcccccg gtcctttgga
     2821 tttagaacgg gggtgtattc tttcctcggc gaatatgccg cagattaaaa atctggaagt
     2881 tatcgcactt ctcaaaaata aatttccgac gaaaatcatc cgccttgaca acgatgctac
     2941 gtgtgcaacg ctggggcaga aattttttgg tgtagcaaaa acttgggaag atttcgccgt
     3001 ctttacgttg gggaccggcg tgggtggtgg tgcgtttttc cacaaccgtt tagagcgcgg
     3061 ctataaaggg aattttttcg aggtggggca catccccgtc gatgcgatgc gggacgcagg
     3121 caggcagtgt ggctgcggaa accgaggttg tttggaggcg tacgcgtctg ccacggggat
     3181 tgcgttctct tataaagagg aaacaggtgt ggatatgagc gcaaaagata ttgctgcgcg
     3241 tgcccgtgaa ggcgacactc acgctcaggc cgcattttca ctcgccgcgc atgcgctcgc
     3301 gttgggcgcg gcggcaatta cccaaacgac gaatatcact tcttttattt tcacag
//
LOCUS       DAOMPD010000295         3349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11611_length_3349_cov_2.935337, whole genome shotgun sequence.
ACCESSION   DAOMPD010000295 DAOMPD010000000
VERSION     DAOMPD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11611_length_3349_cov_2.935337"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLY93_10080"
     CDS             complement(<1..536)
                     /locus_tag="PLY93_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRP69866.1"
                     /translation="MAYRKTKIICTIGPATNTLDKIRKLAEAGMNIARINMSHGDQKS
                     HQEVIQRIKSYNKTAAMPIAILLDTQGPEIRTGDVMENFTLTVGDTIEITAKSENPES
                     KSVITVNYRDMLKDLKPGDRITLDNGIINLDVVEKTDFGLKCRVVDGGVVKSRRHINL
                     PGIRVNLPSITAKDVSDI"
     gene            657..1073
                     /locus_tag="PLY93_10085"
     CDS             657..1073
                     /locus_tag="PLY93_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69867.1"
                     /translation="MDDLYTVTPSRSEKAQEYNRLVRLLAQSGKSNFKKYLYDPLFAA
                     AWERRASDARKMLDHLRASLKNPAYRTTLAPNCKRMVGSAMVEGFPLLGDTVIFFLEI
                     IQMLPQAAESPEAKEFMQILKEPLEAWQKIHDDRSA"
     gene            1074..1571
                     /locus_tag="PLY93_10090"
     CDS             1074..1571
                     /locus_tag="PLY93_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69868.1"
                     /translation="MFDRELNNMEDARIAEALEPVKLGSADTKSHISHEVQMLYQQIL
                     IASRADNFKRAGQLLSRYMIEYSDAEDYAEKDVRKVIDALTLRDPSFSGALHNLLATD
                     LYFRITQGIMKGDLKVAVRMIRKYAMIFEGNPDIRYYYEIDRLERMLYNVITKKNLWK
                     IMKKI"
     gene            1585..1932
                     /locus_tag="PLY93_10095"
     CDS             1585..1932
                     /locus_tag="PLY93_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP69869.1"
                     /translation="MADAQIQIQEKVNGKKAILEIGGELDAKTTPAFKAKLDEVLAKG
                     IDGLLIDCTRLTYVASAGIGAMNAALKTLKDKNGKMALSGLSKEVRDTMDLMFLTKRL
                     PVYTNAAEGAKNI"
     gene            1973..2443
                     /locus_tag="PLY93_10100"
     CDS             1973..2443
                     /locus_tag="PLY93_10100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69870.1"
                     /translation="MQTEITLKISGDVSELKGVREAIKAFLAKIKTEEHNALANVELN
                     ALEESRLVLAVDEALANIIEHGYENKTALAEAQISLTMRQNGSDAEFELCDNGVPFNP
                     LEHKTNTEFYYEEGLEDGLGLIALTILDTAYERKADQNILRIKCALRKEIGKKI"
     gene            2440..>3349
                     /locus_tag="PLY93_10105"
     CDS             2440..>3349
                     /locus_tag="PLY93_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69871.1"
                     /translation="MKKIMKKLWLKILPKISPGLRLKLTAFTLFFVSVLLVAGFLFSY
                     LSLKNQLEESFRKEVRAPEEVVSAHVADLHRFTQGLVQLETFRIRLKQKTAAAQKLRK
                     AVLVKEDSFSNRFRNLAKTFGARVQYTYQTRRFDTYYSTYLTDKNLRDFETQLKAAID
                     TSLTQPLDTRTFTRWKTIAARVARDEISAAQAEEKNASGSQSKTIAARKNAAAKNRSI
                     LMNDLSRVFDTYLQARLDRMQFSRQTIRLLSYSTELETLFGGSDKNGKHEKEKAKKKV
                     NMPRPLFDTGIHGVADTPENWALLRNA"
BASE COUNT          983 a          730 c          815 g          821 t
ORIGIN      
        1 aaaatatcgg aaacatcttt ggcggtgatc gacggcaaat tgacgcggat gccggggagg
       61 ttgatatgcc ggcgcgactt caccacgcca ccgtcgacca cgcggcattt gagaccaaaa
      121 tcggttttct ccaccacgtc gagattaata ataccgttgt cgagggtgat acggtcgccc
      181 ggttttaagt ctttgagcat gtcgcggtag ttgaccgtga tgacgctttt cgattcggga
      241 ttttccgatt ttgcggtgat ttcaatggtg tcgccgacgg tgagcgtgaa attttccatc
      301 acgtcgccgg tgcggatctc agggccttgc gtatcgagta aaatcgcaat gggcatggcc
      361 gccgttttgt tatacgactt aatccgttgg attacctctt ggtgcgattt ttgatcgcca
      421 tgagacatat tgatgcgtgc aatgttcatg cccgcctcgg cgagcttgcg tattttgtcg
      481 agggtgttgg tggcggggcc aatggtacaa atgattttag ttttccggta agccataaga
      541 gtagtatgcg attagaggat aggccgcaaa acataaaata gaaagtggta gataattcta
      601 taaactaaaa aagccttgtc cgtgccgttt taagctccac cttaaccgag cttgttatgg
      661 atgacttgta tacggtgacc ccctcacgct ctgaaaaggc gcaagaatat aatcgtctcg
      721 tgcgcctttt agcgcaatcg ggtaaaagta atttcaaaaa gtatttatac gatccccttt
      781 ttgcagccgc gtgggaaagg cgtgcgagcg atgcgcgcaa gatgctcgat cacctgcgtg
      841 cctcgctcaa aaatcctgcg taccgcacaa cgcttgcccc caattgtaaa cggatggttg
      901 gttcggcaat ggtcgagggc tttcctcttt tgggtgatac ggttattttc tttttagaaa
      961 ttattcaaat gctgccgcaa gcggcggagt cgccagaggc gaaagagttt atgcaaatac
     1021 tcaaagaacc attggaagcg tggcagaaaa tccacgacga tcggagcgct tagatgttcg
     1081 atcgagaact taacaacatg gaagacgcgc gcatcgccga ggcgcttgag ccggtaaagc
     1141 tggggagcgc cgacaccaaa tcgcacatct cgcatgaagt acaaatgctg tatcaacaaa
     1201 ttctcatcgc ctcacgcgcc gataatttta agcgagcagg tcagcttttg tcgcgctata
     1261 tgatcgagta tagtgacgca gaagactatg cggaaaaaga tgtacgtaaa gtcatcgatg
     1321 cgttgacgct gcgcgatcca tcgtttagcg gcgcactcca taatctttta gcgactgatt
     1381 tatattttcg tatcacccaa ggcattatga aaggggatct caaagttgca gtccgcatga
     1441 ttcgtaaata cgcgatgatt tttgaaggca accccgacat ccgttattat tacgaaatag
     1501 atcgactgga gcgcatgctt tacaacgtga ttactaaaaa aaacttatgg aaaataatga
     1561 agaaaatata aaaggaagta caaaatggca gatgcacaaa ttcaaattca agagaaagta
     1621 aacggaaaaa aagcgattct tgaaattggg ggcgaactcg acgcaaaaac gacccctgca
     1681 ttcaaggcga agctcgacga agtactcgca aagggtatcg atggccttct catcgattgc
     1741 acacgactca cctacgttgc ctcggcaggt atcggtgcga tgaacgctgc attgaaaacg
     1801 cttaaagata aaaacggaaa aatggcattg tcgggtcttt ccaaagaagt gcgcgacacg
     1861 atggatttaa tgtttttaac aaaaagattg cctgtgtaca caaacgctgc cgaaggtgca
     1921 aaaaatattt aatcgagttc ctctttaaat aattgagtgg agcgattaaa ttttgcaaac
     1981 agagattaca cttaaaattt caggcgacgt atctgaatta aaaggagtgc gcgaggcgat
     2041 aaaagcattt ttggcaaaaa taaaaacaga agaacacaat gccttggcga atgttgaatt
     2101 aaacgctctg gaagaaagcc gcctcgtttt agccgtcgat gaagcgctgg caaatattat
     2161 cgaacacggg tatgaaaaca agacagcgtt agctgaggcg caaatttcgc tcacgatgcg
     2221 gcaaaacggg agcgatgcgg aatttgaact ttgcgataac ggtgtgccct tcaacccgtt
     2281 ggagcataaa accaacaccg aattctatta tgaagaaggt ttggaagacg gtttgggact
     2341 cattgcgctg acgattctcg acaccgcgta tgaaagaaag gcagaccaaa acattttacg
     2401 gataaaatgt gcgctgagaa aagaaatagg gaaaaaaata tgaaaaaaat tatgaaaaaa
     2461 ttatggctaa aaattttacc taaaatctcc ccagggcttc gactcaagct taccgcgttt
     2521 actctctttt ttgtctcggt gttgcttgtg gcgggttttc ttttttctta cttgagtttg
     2581 aaaaatcaac tggaggaatc tttccgtaaa gaagtgcgcg cgcccgagga agtggtgagc
     2641 gctcacgttg cggatttaca tcgctttact caaggacttg tgcagttaga gacatttcgt
     2701 attcgcttaa aacaaaaaac ggctgcggcg caaaaattac gcaaagctgt tttagtaaaa
     2761 gaagattcgt tctcaaatcg gttccgtaat ttagcaaaaa cgtttggtgc acgcgtgcaa
     2821 tacacctatc aaacgcgccg ctttgacacg tattatagca cctacctcac cgataagaac
     2881 ttgcgcgatt ttgaaactca gttaaaagcc gcgatcgata cgtcgctcac gcagccttta
     2941 gacacgcgta ctttcacccg ttggaaaaca atcgctgcgc gcgttgcacg cgacgaaata
     3001 agcgccgcgc aagccgaaga gaaaaacgca tcggggtcgc aatcaaaaac aatcgctgca
     3061 cgaaaaaatg cggcggccaa aaaccgatcg attttaatga acgacttgag ccgtgttttc
     3121 gatacttact tgcaggcgcg tttagaccgg atgcaattct cgcggcaaac aattcgtctt
     3181 ttatcgtaca gcaccgaact cgaaacgctt tttggcgggt ccgacaagaa tggaaagcac
     3241 gagaaagaaa aagcaaaaaa gaaagttaat atgccgcgcc ccctctttga caccggtatc
     3301 cacggggttg ccgacacgcc ggaaaactgg gcgcttttac gcaatgccg
//
LOCUS       DAOMPD010000296         3349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11613_length_3349_cov_2.507286, whole genome shotgun sequence.
ACCESSION   DAOMPD010000296 DAOMPD010000000
VERSION     DAOMPD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11613_length_3349_cov_2.507286"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLY93_10110"
     CDS             complement(<1..768)
                     /locus_tag="PLY93_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRP69872.1"
                     /translation="MKKNIRWIYTAIVLILIGGIVPLISIDTDARRQSIQDRFNKIKA
                     QNDAKLEQIRKEVTDMNAEIKRRSLSFQVEINEKMKEKMANITGLKPPKPPKPNPKPE
                     PEPEPAPPPPVPDEDKRSQCDPSAEAFDWRSQGVVPAIRDQKSCGDCYMFAAMASYET
                     ALKIRNNHEADMAEQYFLNCTREFGCDGGWYGTVWTKMTKMKGDVETNYPYQAKKGIC
                     KNITPAGDYRVVRSGYVSQGVGSPQAIKNALCEHGVLA"
     gene            965..1441
                     /locus_tag="PLY93_10115"
     CDS             965..1441
                     /locus_tag="PLY93_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69873.1"
                     /translation="MKKTGYLALFVIAFVVSYCGGTKGMQDISQGEGSIDEIEGGFIG
                     DDKLIVKGAGVSKKGVKTSLQKEATAKEAARMDAMSKVIEICRGANMQAAATVENAEL
                     ANSVIGKDLAGRIKGAQVKKSNCKPDGEYVGCKVIMEITKKGIKKDCELAMADLAK"
     gene            complement(1487..3202)
                     /gene="argS"
                     /locus_tag="PLY93_10120"
     CDS             complement(1487..3202)
                     /gene="argS"
                     /locus_tag="PLY93_10120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRP69874.1"
                     /translation="MTLRFFVRALLTKAAQKAATTLGVTLPENFSPRVDYTPSIALGH
                     YASPAAMELAKVFKQAPRVIAESICAELDKAAFAKVEIASAGFINFTLADEPLRSNVN
                     FASVHEWLMGVQKEIPTEKIIFEYVSANPTGPMNIVSARAASVGDSLTRVLKALGQDV
                     WREYYVNDYGNQVDMLGLSFAYRYLQKFIAVDLPEDCYQGEYIVHYLEKILADEKIPD
                     AILLTQERVAALAKEAWLSQAASYFSPLGVRAILHEQKKDLQTFRAEFDHFFSEASLH
                     QKGAVVDIAEKLKTKGKIKTTDGAQVFLSTEYGDDKDRVVLRSDGRPTYLLADIAYHY
                     NKVERGFNRIVDIWGPDHHGYIARLTGALKALDFENFKVIIAQQVNLLENGEPVVMSK
                     RLGKFQTMRDLIEKIPVDVCRYFFVVRSLSTHLDFDLTLALDQSKKNPVYYIQYAHAR
                     IHSIAREVNFDLTEPLHLDTILANANFSENIEREELFFFALRFPELLQDIARNYEIQR
                     LPEYLYTLAAYFTAFYHGKNNRVKDVVQSNPHEGTGLLMLSALTAQVLETGLSLLGIS
                     APKEM"
BASE COUNT          895 a          735 c          798 g          921 t
ORIGIN      
        1 ggcgagcaca ccgtgttcgc agagcgcatt tttgatggct tgtggcgagc cgacgccttg
       61 cgacacgtag ccagaacgca ccacacggta atcgcccgca ggggtgatgt ttttacatat
      121 cccttttttg gcttgatagg gatagttggt ttcaacgtcg cctttcattt ttgtcatttt
      181 tgtccaaaca gtgccatacc agccgccgtc gcagccaaac tcacgcgtgc agtttaagaa
      241 atactgctcg gccatgtcgg cttcgtggtt attgcggatt ttaagcgcag tttcgtaaga
      301 tgccatcgca gcaaacatat agcagtcgcc gcaagatttt tgatcgcgga ttgctggcac
      361 gacaccttgg ctgcgccagt caaacgcctc agcgctggga tcgcactggc tacgcttgtc
      421 ttcatccggc acaggcggcg gtggagcagg ttctggttcc ggttctggtt ttggattagg
      481 cttaggaggc ttgggcggtt ttaagccggt aatatttgcc attttttctt tcattttttc
      541 gttgatttca acttgaaacg aaagcgagcg ccgttttatt tcagcgttca tatcggtgac
      601 ttctttgcgg atttgttcga gcttagcatc gttctgcgct ttgattttat taaagcgatc
      661 ctgaatgctt tgccttcttg catcggtatc aatggagatg agtggaacaa ttcccccaat
      721 gaggattagc acaattgcag tgtaaatcca cctaatgttc tttttcatac tctatttaga
      781 cgtgcgaagc tacgatgtaa agagaatttt tttatacgac taaaaaattt tacgaccttt
      841 ttcagaccat acagcgtcta cataaagaac aactttctct tataggcttg acttgttaaa
      901 gaaaagctca gacatagaca agcccaaagt ataataaagt tgggaaataa aattaggaga
      961 ctctatgaaa aaaacaggtt atttagcgtt atttgtgatt gcttttgtgg tttcatactg
     1021 cggcggcaca aaaggtatgc aagacatcag ccaaggcgaa ggctcaatcg atgaaatcga
     1081 aggtggtttc attggtgacg ataagctcat cgttaaaggc gctggtgttt caaaaaaagg
     1141 tgtaaaaaca tcgcttcaaa aagaagcgac tgcgaaagaa gcagcacgta tggacgcaat
     1201 gtcgaaagta atcgaaattt gccgtggtgc taacatgcaa gcggctgcaa ctgttgaaaa
     1261 cgcagaactc gcaaactcag tgattggtaa agacttggct ggccgtatca aaggcgctca
     1321 ggttaaaaaa tcaaactgta aacccgacgg tgaatatgtt ggctgcaaag tcatcatgga
     1381 aatcaccaaa aaaggtatca aaaaagactg cgaattggca atggccgacc tcgcgaaata
     1441 aaatcattca gtatattttg cgcgtcagac gcgcagaata tacgttttac atctctttcg
     1501 gcgctgaaat tcctaaaagc gacaaccccg tttctaaaac ttgcgcagtg agtgcagaga
     1561 gcatcaaaag cccagtacct tcatgtgggt ttgactgaac cacatcttta acacgattat
     1621 ttttaccatg gtagaacgct gtgaagtacg cggcgagcgt atagagatat tcaggcaagc
     1681 gttggatttc ataattgcgg gcaatgtcct gcaaaagctc ggggaaacgt aacgcaaaga
     1741 aaaaaagttc ttcgcgctcg atattctctg aaaaatttgc attcgctaaa attgtatcca
     1801 aatgcagcgg ctctgttaaa tcaaaattaa cctcgcgcgc aatcgaatgg atgcgtgcat
     1861 gcgcgtattg gatataatac acaggatttt ttttggattg atcgagcgcg agcgttaaat
     1921 caaagtcgag gtgtgtgctc aaactgcgca ccacaaaaaa atagcggcac acatccacgg
     1981 ggattttttc aatgagatcg cgcatcgttt gaaatttacc caaccgcttc gacattacca
     2041 ccggttcgcc gttttcgagc aaattcacct gttgcgcaat aatcactttg aaattttcaa
     2101 agtcgagcgc tttcaaggct cctgtcaggc gtgcgatata gccgtggtgg tcgggccccc
     2161 aaatatcgac gatgcggtta aagccccgtt cgactttgtt gtagtggtag gcaatgtcgg
     2221 cgagtaagta tgtggggcgc ccgtccgagc gcagcacaac acggtcttta tcgtcgccgt
     2281 attcggtgct caaaaaaact tgtgcgccgt ctgttgtttt tatttttcct tttgttttca
     2341 gtttttcggc aatgtcgacc acagctcctt tttggtggag cgacgcctcc gaaaaaaaat
     2401 gatcgaactc tgcacggaat gtttgtaaat cctttttctg ctcgtgtaaa attgcgcgca
     2461 ctcctaaagg agaaaaataa gaagcagctt gcgatagcca cgcttctttg gcgagagcag
     2521 cgacgcgctc ttgagttaaa agaatagcgt cagggatttt ttcatcggct aaaatttttt
     2581 caagatagtg gacaatatat tccccttggt aacaatcttc gggtaaatcg acggcaataa
     2641 atttttgtag ataacggtat gcaaacgaga gccccagcat atccacttgg tttccataat
     2701 cgttcacgta gtattcgcgc caaacatctt gccccaatgc ttttaagact cgggtgaggc
     2761 tgtcgcccac cgaggcagcg cgcgccgaca caatgttcat ggggccggtg ggatttgccg
     2821 atacgtattc aaaaataatt ttttcagtgg gaatttcttt ttgaactccc ataagccatt
     2881 cgtgaacgct tgcaaaattt acgttggagc gcaggggttc atcggcgaga gtaaagttga
     2941 taaaccccgc actggcaatt tctacttttg caaaagcggc cttgtcgagc tcggcgcaaa
     3001 tgctttcggc aattacgcgc ggcgcttgtt taaaaacctt agcaagctcc atcgcggcag
     3061 gacttgcgta gtgccccaat gcaatcgaag gggtataatc cacacgcgga gaaaaatttt
     3121 ctggtaacgt cacgcccagc gttgtggcgg ctttttgcgc cgctttggtc aaaagcgcgc
     3181 gtacaaaaaa tcgtaatgtc atacgcctat ctcgatttac gatttagagc gaaaactcaa
     3241 acccttcggg aatcattttc gcggaaggga aaattttttg cgcgtcggct tccatttgca
     3301 aaagcgtatg atacgtgtgc aatgtgtcgt ggtggatcat gtgtagatt
//
LOCUS       DAOMPD010000297         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11659_length_3336_cov_2.026516, whole genome shotgun sequence.
ACCESSION   DAOMPD010000297 DAOMPD010000000
VERSION     DAOMPD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11659_length_3336_cov_2.026516"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLY93_10125"
     CDS             <1..381
                     /locus_tag="PLY93_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HRP69875.1"
                     /translation="NDTVLRGQVLIAPGNKHTVLKRSGHRYYVDVIEGDLVNRHRPSV
                     DVLFRSVANNAGKNAVGVIMTGMGDDGAHGLLEMKKSGAYTIAQDEESCVVYGMPKEA
                     VKCGAVDKILPLENIAAEVLRKTV"
     gene            400..777
                     /locus_tag="PLY93_10130"
     CDS             400..777
                     /locus_tag="PLY93_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP69876.1"
                     /translation="MEEKKKAMIVDDSRLTQKQLAQILTDCDIDVTDIAANGVEAVDK
                     VKANPNAFDLITLDITMPQLDGVSALKEIMQVNKNLRVLMVSAIGKESVIKVCMATGA
                     RNFIVKPFHAEKVKEIVKATLAA"
     gene            921..>1803
                     /locus_tag="PLY93_10135"
     CDS             921..>1803
                     /locus_tag="PLY93_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRP69877.1"
                     /translation="MQKIYSLSVIFFSIAGILPLNAAPRKGQLTPAAKTAPAAVVENK
                     VEVTAKTSEADAQAQEKKNWYDSIKISGMIRVRPESKQNFGFDNTQKYDFVGQKTWLT
                     AEKTFSDKSKAVVTLQDVRIWGGQNPTNTDTGAELQAVDVREAYLLLKNFLFTPFDLQ
                     VGRQKIGYGDEVFIGVSDWGNTGRSFDGVRLYLNSDKNNLQIFSTLLQGGRSNDPMNP
                     NSALANPLPNSGPGSKLNQYFSGIYDAWKIHPMFMLDTYFFARNQDNKITANQLYTGG
                     VRISNRTEAGNAAPKDAI"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1814..2370
                     /locus_tag="PLY93_10140"
     CDS             <1814..2370
                     /locus_tag="PLY93_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRP69878.1"
                     /translation="YSINTAYQSGFNAGKKIEAYAVVGFLGVIFNAGAKMRVGGQGAY
                     ASGGDNSGSTYHTFDPLYPTPHMTYGQADMASLRNLSGFGVDYTVWFTPALNLRIDYW
                     YATRTSTSDGWYAVTGKEATTAATLAGATQLYHEIDARLDYKARDFLLFQAGYAYAMR
                     GKALKAAGKNADYQYAYLMSTILF"
     gene            2602..>3336
                     /locus_tag="PLY93_10145"
     CDS             2602..>3336
                     /locus_tag="PLY93_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRP69879.1"
                     /translation="MADDAKKFTTKATEKNGMEVMLDVLEKNGVDICWGYPGGAILPF
                     YDALYSSKIKHILTRHEQGAVHAAEGFAKATGRLGMCISTSGPGATNLLTGIADAKLD
                     SVPVLALTGQVATGAIGTDAFQECDTYGMSIPITKYNALIRHADDVARITQEAITVAT
                     SLRPGPALIDFPKDVQTGKTKITKPEMRLSERHYKKPKLKGDFDRLIDALTKAERPLL
                     YVGGGAVISNAYKEVRALAEKADIPVF"
BASE COUNT          928 a          797 c          838 g          763 t
ORIGIN      
        1 aacgacaccg ttttgcgcgg ccaagtttta attgccccgg gtaacaaaca caccgtcttg
       61 aagcggagcg gccaccgcta ttacgtcgac gttatcgaag gcgatttagt gaatcgccac
      121 cgtccatcgg tcgacgtact tttccgttcg gttgccaata atgcaggaaa aaatgccgta
      181 ggagtcatca tgaccggcat gggcgacgac ggcgcgcacg ggcttttaga gatgaaaaaa
      241 agcggtgcgt acacaatcgc acaagacgaa gaaagttgcg ttgtgtatgg tatgcctaaa
      301 gaggcggtaa agtgcggtgc cgtcgataaa attttaccgt tagaaaatat tgcagcagaa
      361 gtgctgagaa aaacagtttg agaaaaaaag gggattacta tggaagaaaa gaagaaagcg
      421 atgatcgtgg acgattcacg gctgacgcaa aaacagttgg cgcaaatttt aaccgactgc
      481 gatatcgacg ttaccgacat cgcggcaaac ggagtggaag cagtggataa agtgaaagcc
      541 aatcccaacg cgttcgatct catcactctc gacatcacca tgccgcagct cgacggcgtc
      601 tctgcgctca aagagattat gcaagtcaat aaaaatttac gcgtgctgat ggtgagcgcc
      661 atcggtaagg aaagtgtgat taaagtttgt atggcgaccg gcgcacgcaa ttttattgtg
      721 aaaccgtttc atgcggaaaa ggtgaaagaa attgtaaagg ccacattagc ggcttaaaga
      781 gcgggttggc gcacagactc gtaagcggtg agttgattat agacttattt ctctgttttt
      841 gactcataaa ttgttcgtcc cactatttga taaaaaaagt tttaccgcga tcaaacgcga
      901 tcaattttaa ggagtgcttt atgcaaaaaa tttattcatt gtcggtgatt tttttctcga
      961 tcgctggcat ccttccgctg aatgctgcgc cacgaaaggg gcagcttacg cctgcggcaa
     1021 aaactgcgcc agcggccgtg gtcgaaaaca aagtagaggt gacggcgaaa acaagcgagg
     1081 cagatgccca agcgcaggag aaaaaaaact ggtacgactc gattaaaatt tccggtatga
     1141 ttcgggtgcg ccctgaatca aaacaaaatt ttggattcga caacactcaa aaatacgatt
     1201 tcgtcggtca gaaaacatgg ctcaccgcgg aaaaaacatt ttcggataaa agtaaggctg
     1261 tggtgacgct gcaagacgtg cgcatttggg gcggacaaaa tcccaccaac accgacaccg
     1321 gcgccgaact ccaagcggtc gacgtgcgcg aggcgtattt actcctcaag aatttcttat
     1381 tcactccgtt cgatttacaa gtgggacgcc aaaaaatcgg atacggcgac gaagtgttta
     1441 tcggcgtttc cgactggggg aataccggac gttctttcga tggcgttcgc ctctacttaa
     1501 atagcgataa aaataatcta cagattttct caacgctgct ccaaggggga cgctctaacg
     1561 acccgatgaa ccccaattct gccttggcaa atcctctccc caactcaggg ccagggtcaa
     1621 aattaaatca atacttcagc ggcatttacg atgcgtggaa aatccatccc atgttcatgc
     1681 tcgacactta ttttttcgca cgcaaccaag ataataaaat cactgcaaac cagttgtaca
     1741 cagggggcgt ccgaattagc aaccggacag aagcagggaa cgcagcgcct aaagacgcga
     1801 tctnnnnnnn nnnattattc tattaacacc gcataccaaa gtggttttaa cgcaggtaaa
     1861 aaaattgaag cgtacgcagt ggtgggtttc ttgggtgtga tttttaacgc gggcgcaaag
     1921 atgcgcgtgg gtggccaagg cgcgtacgcc agcggcggcg acaacagcgg ttcaacgtac
     1981 cacacgttcg atcctcttta ccccacaccg cacatgacgt atggtcaagc cgatatggcg
     2041 agtttgcgta acctttcggg ctttggtgtt gactacaccg tgtggtttac ccctgcactc
     2101 aatttgcgga tagattattg gtatgccaca cgcaccagta catccgatgg atggtatgca
     2161 gtgacgggta aagaggcgac aaccgccgct acgctcgctg gtgcaacgca gttgtatcat
     2221 gaaatcgacg cacgcttaga ttacaaagcg cgcgactttt tactttttca agcaggatat
     2281 gcatacgcga tgcgcggcaa ggctttgaaa gctgcgggta agaatgcgga ttatcaatac
     2341 gcgtatttga tgtccacgat acttttttaa tacgattata gcggtagtta attggttgga
     2401 attaactacc gcctaagttc tatgagcctg tgcgccaact cgctgagaca atttttttgc
     2461 aaactttcgt gtttttgagc gaaccgcgta caaaagtttg taaaaaaatt gccgctttat
     2521 acacgctacg cgagttgatg cacaggttcc tataacctaa ttaacctttg acggcgtgtc
     2581 ttaacacgcc gtcacggagg tatggcagac gacgcgaaaa aatttacaac gaaggcaaca
     2641 gagaaaaatg gcatggaagt catgctcgac gtgttggaaa aaaatggcgt cgatatttgc
     2701 tgggggtacc caggcggagc cattttacct ttttacgacg cgctctactc ttcaaaaatt
     2761 aaacatattt taacccgcca cgaacagggg gctgtacacg ccgccgaagg gtttgctaaa
     2821 gccactggcc gcttgggtat gtgtatttca acctcaggcc ccggcgcgac gaacttgttg
     2881 accggcatcg ccgacgctaa gctcgacagc gtgccggtgt tggcgctcac cgggcaagtg
     2941 gcgacggggg ccatcggcac cgacgcattc caagaatgcg acacgtatgg catgtcgatt
     3001 ccgattacaa agtacaacgc actcatccgc catgccgacg acgtggcgcg catcacccaa
     3061 gaagccatca cggtcgctac ttcgctgcgc ccggggcctg cgctcattga tttccctaaa
     3121 gatgttcaaa ctggtaaaac aaaaattact aaacctgaaa tgcgcttgag cgagcggcat
     3181 tacaaaaagc ccaaactcaa aggtgacttc gaccgcctta tcgacgcgct caccaaagcc
     3241 gaacgcccgt tgctttacgt gggcggcggt gcggttatct cgaatgcgta taaagaagtg
     3301 cgcgcactcg ccgaaaaagc agacattccc gttttc
//
LOCUS       DAOMPD010000298         3326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11696_length_3326_cov_2.168756, whole genome shotgun sequence.
ACCESSION   DAOMPD010000298 DAOMPD010000000
VERSION     DAOMPD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11696_length_3326_cov_2.168756"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="argB"
                     /locus_tag="PLY93_10150"
     CDS             <1..409
                     /gene="argB"
                     /locus_tag="PLY93_10150"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRP69880.1"
                     /translation="PQDIQTTVLTTLKQGGFIPVIAPIGFDREGNALNINADTMAGAI
                     ASALKAEKLLLLTDTPGVMYEGKTLTRLTPHEVDSLQQQEIISGGMIPKTNCAKNAVE
                     EGVAAAHIIDGRVPHALLLEIFTDNGVGTMVVG"
     gene            442..1203
                     /locus_tag="PLY93_10155"
     CDS             442..1203
                     /locus_tag="PLY93_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69881.1"
                     /translation="MKKLLLLILSASFAPSFAYVSSNVAGQTGLIATPTARINWENSG
                     RDFGVTTGYTYFNNNATYNVPQVNLSLFRRWEIGGAYNAAPGKDNNDALLHTKLRFSP
                     WSGTGASELAIGGSYQSLNSNPGTTAGQVYLAATYQASLFGMEADTTLVVGKTFGKSI
                     PSGDVDFSMGFDLDFFPSLFKGYVRWLNELANYDYLYANAPTRALSRGAFNTGFRIPV
                     LKHMSNLKFDVTIKMTDALDNERGFGIGAVVGGSF"
     gene            complement(1331..>3326)
                     /locus_tag="PLY93_10160"
     CDS             complement(1331..>3326)
                     /locus_tag="PLY93_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRP69882.1"
                     /translation="IATSGVLSIAAARITRIHPAYFFLLSGAVLPFVLPFTFPSQSLL
                     VELAEVGALFVVFLAALEIEWDLRFVWQARTIFTSLVLQAMTILPVMAIMYFFITPHL
                     STALTVAFLAAMHAPDRRQSKFSESFRADRVASDVAFMGFTSEVSALLAIAVLSAYTK
                     EITFAADIVKSLVGVVLMLLVLISIFPPLIRFLVRRVGEESYAFFYLLLLLLIVVVGV
                     LIRVDIEPLFGIYAAGFVLKRFISEGSRALERMRFTGHSMIVPAFYIALGIAGALFSS
                     FQADALVYALSILLTTLIARAAFAYIVRMRDRRTRISLSQLIRKNPLVLVLVYIAYAR
                     GLISSPALHTVLYYLILNEVLAVYLNRFQRSEKREPLIENNTRILVPVANPESMMPLL
                     TLAGHMGHSDAPAKIFPLNVVPDTTESAMRIRRVEAQFQELKKLYATREEDLELTARI
                     DNDRIHAVTHAARELITEKIILGLGQITTLQKPHGYSFLDTLAEKATQQTVIAAHLPV
                     ELAVSTELNVLVANERMILSQEIWLPIVLNLSRRLKATPVFLGEAQVLPHITEYLNEL
                     DPRRKYPIRAGNLHAGLDLLTLSNNDSALWIAILERPAFAPEESIHVRLPEMMLRAFS
                     ERNFLLLYPQMTAIPKKFKKSKKGTWRRVKNFFGFSKIIF"
BASE COUNT          929 a          874 c          804 g          719 t
ORIGIN      
        1 tccacaagat attcaaacga cagtactcac cactctcaaa caaggaggat ttatccccgt
       61 catcgcgccg attggtttcg acagagaagg caacgcactc aacatcaacg ccgacaccat
      121 ggcgggcgcc attgcatcgg cgctcaaagc agaaaaattg ttactcctca ccgacacgcc
      181 gggggtgatg tacgaaggta aaacgctcac ccgcctcacc ccgcacgaag tcgactcact
      241 gcaacaacaa gaaattattt caggtggcat gatccccaaa acgaactgtg ccaaaaacgc
      301 tgtcgaagaa ggagtggccg ccgcgcacat catcgatggc cgcgtaccgc atgcgctcct
      361 ccttgaaatt tttacagata atggtgtcgg tacgatggtc gttggttgac agcgtatttc
      421 atacctcatt gctgccattc tatgaaaaaa ttattactat taattttaag cgcctcgttt
      481 gcgccaagtt ttgcgtacgt ttcttccaac gtcgcaggac aaacaggatt aatcgcaacg
      541 ccaaccgcac gcattaactg ggaaaatagc ggacgcgact ttggcgtcac caccggatac
      601 acctacttca acaacaacgc gacgtacaac gtgccgcaag tcaacctctc tcttttccgc
      661 cgctgggaaa tcggtggtgc atacaatgca gcccccggca aagacaacaa cgatgcgttg
      721 ctccacacca agttacgctt ttcaccctgg tcgggaacag gtgcatccga gttagccatc
      781 ggcggcagct accaaagcct caactcgaat ccaggcacaa cggcaggtca agtttattta
      841 gcagcgactt accaagcgag ccttttcggc atggaagccg acaccacatt agtcgtgggt
      901 aaaacttttg gtaaatcgat ccccagtggc gacgtggatt tttcgatggg attcgatctc
      961 gattttttcc ccagcctttt caaaggttat gtgcgttggc tcaacgagtt ggcgaactac
     1021 gattatctct atgctaacgc acctacacgc gccctctcgc gcggggcatt caacaccggt
     1081 ttccgtatcc ccgttttgaa gcacatgtca aacttgaagt tcgatgtcac gattaaaatg
     1141 accgatgcgt tagacaacga acgcggattt ggtatcggcg ctgtcgtcgg tggcagtttc
     1201 taacagactg ttaaaaaaca gacccaagca aggtgttaaa actgaacgta gccgcagaca
     1261 gaccattggg ggctgttttg cgagcgattg ctaccgacaa tcgcagcgtc tgcggcacgc
     1321 caacacccta ctaaaaaata attttagaaa agccaaagaa attcttcact cgtcgccaag
     1381 ttcctttttt tgactttttg aatttctttg gtatcgccgt catctgcggg tagagtaaca
     1441 aaaaattacg ttcgctaaac gcccgcaaca tcatctccgg caatcgaacg tggatggact
     1501 cctcgggcgc aaaagccggt ctttctaaaa tagcaatcca caacgcgcta tcgttgttgc
     1561 tcaacgtgag tagatccaac cctgcgtgga gattccccgc acggatgggg tatttgcggc
     1621 gtggatctaa ctcgtttaaa tactcggtga tgtgcggcaa cacttgcgcc tcgcctaaaa
     1681 aaaccggagt ggctttgaga cggcgcgaga gattgagcac aatcggcagc catatttctt
     1741 gggagagtat catacgttcg ttggcgacga gcacgttaag ttcggtcgag accgctaact
     1801 ccaccggcaa gtgtgcagcg atgacggttt gttgcgtcgc tttttcagcg agggtgtcta
     1861 aaaacgagta gccgtgcggt ttttgtaacg tggtaatctg ccccaagccc aaaataattt
     1921 tttcggtaat aagctcgcgc gctgcgtgtg tcactgcatg gatgcggtcg ttgtcgatgc
     1981 gcgctgtgag ctctaaatct tcctcgcgcg tcgcgtagag tttttttagc tcttggaatt
     2041 gcgcctcgac gcggcggatg cgcattgcac tttcagtagt gtcaggcacc acgttgagtg
     2101 gaaaaatttt tgccggcgcg tcgctatgcc ccatgtgacc ggcgagcgtc aagagaggca
     2161 tcatcgattc ggggttggcg acaggcacta aaatacgagt attattttca atcagcggtt
     2221 ctcttttttc actgcgctgg aaacgattca agtacaccgc taaaacttcg ttgagaataa
     2281 gataatacaa tactgtatgc aacgccggtg acgaaatcaa tccgcgcgcg tatgcaatgt
     2341 aaaccaacac caacaccaac ggatttttgc ggatgagttg gctcaatgaa attcgcgtgc
     2401 gtcggtcgcg catacgaaca atatacgcaa acgctgcgcg cgcaatcaat gtcgtcaaca
     2461 gaatcgacaa cgcgtaaacg agtgcatccg cctgaaacga ggagaaaaga gcgccggcaa
     2521 taccgagcgc gatataaaac gcaggcacaa tcatactgtg cccggtaaac cgcatgcgct
     2581 ccaacgcgcg cgaaccttca gagataaaac gcttgagtac aaagcccgca gcgtaaatac
     2641 caaagagcgg ttcgatatct acccttatca gcacgccaac cacaacgatg agaagcaata
     2701 aaagaagata aaaaaacgca tacgactctt cgcccacacg gcgtactaaa aaacggataa
     2761 gcggcggaaa aatggagatg agcacaagga gcatcaatac cacccccacc agcgatttga
     2821 caatgtcggc ggcaaaggtg atttcttttg tataagctga caaaaccgca atggccaaaa
     2881 gcgcactcac ttctgacgtg aaacccataa acgccacatc gctggcgaca cggtcggcgc
     2941 ggaacgattc agaaaattta ctctggcgtc tgtcgggggc gtgcatcgcc gccaaaaaag
     3001 ccactgtgag agccgtcgat aaatgcggag tgataaaaaa atacataatc gccataacgg
     3061 gaagaatcgt catagcttgc aaaacaagag aggtgaaaat ggtgcgcgcc tgccacacaa
     3121 agcgcaaatc ccactcaatt tcaagcgcgg ctaaaaaaac cacaaaaagc gcgcccacct
     3181 cggccagttc caccaagagg ctttgcgacg ggaaagtaaa cggtagaaca aatggcagga
     3241 ctgcccccga aagcagaaaa aaatacgcgg ggtgaatacg agttatacgg gccgctgcaa
     3301 tactcagcac accgctcgtg gcgata
//
LOCUS       DAOMPD010000299         3325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11702_length_3325_cov_1.843425, whole genome shotgun sequence.
ACCESSION   DAOMPD010000299 DAOMPD010000000
VERSION     DAOMPD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11702_length_3325_cov_1.843425"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..>767)
                     /locus_tag="PLY93_10165"
     CDS             complement(140..>767)
                     /locus_tag="PLY93_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP69883.1"
                     /translation="PKLDAIVLSHNHYDHTESDTLKFLAARDNPPVFLPLRVQPLVKN
                     FGFDKIYELDWWQEVALEKFKITCLPSKHFSGRSATDRNQTLWTSMMISFNDPKKNVY
                     FAGDTAYSPHFTEIAHEFSDIDLTLMPIGAYLPRWFMREVHVSPDESIRAFEDLNAKK
                     LLAIHWGTYDLGDEEWDQPKRDAITAAQIRRLDFARICITDIGAVTLF"
     assembly_gap    768..787
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<788..1199)
                     /locus_tag="PLY93_10170"
     CDS             complement(<788..1199)
                     /locus_tag="PLY93_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69884.1"
                     /translation="MTRLLQAIKNLPQYTTEHHNGERFRNIWGPHPDSEFRKYDAQSG
                     DLKYRALDVAAWALRKGIQPRERGTPARVHPPTDAELLPSPIEARAIWLGHATVLLQA
                     GGLNIITDPVFGERVSPVKFVGPKRLTGHALELER"
     gene            complement(1205..2881)
                     /locus_tag="PLY93_10175"
     CDS             complement(1205..2881)
                     /locus_tag="PLY93_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRP69885.1"
                     /translation="MSIKPDPKSIPRPWVKHYDKHVPETMEYSSKSLPELFEESAKEH
                     ATRVGVKFLGKSICYAEIEKSATAFAAFLEAKGIGKGDRVFLMLPNSPHFIVAYYAAL
                     KRGAIVVPASPLDTASEAEYKFKNSGAKVIVYLDLLHSKLSDLLQKDTTTPTVVCDVA
                     DYLSAVKRFFFRLKKATLKIPLPDGKRTASVKYKKVLKEFNDKTPTKIVVKNSEPAVI
                     IYTGGTTGVSKGVVLSHYAMVVNMTQAKNWGDLTLDDVGLCVLPFFHGFGMSVGLNVS
                     LSSGGTMVLMPRWDAGQAIKHFEKDGITVYAGVPTMYNAMLNHKNFAKMKNAKLRGCF
                     VGAAPVPEALKVAFKEKTGGTLIEGYGLTESVTAKCANPYHGLKKEKSIGIPWPDTLM
                     AIVDDTTGELLPPGKDGEIILHSPDLMLGYWQNEEATRATIKNGWLYTGDIGRMDEDG
                     YFAIVDRKKDLIISGGYNVYPAEVEDVLYKHPAVLEACVVGTKDDHLGEVGRAFIALK
                     EGMSVSADELKNFLIDKLIKYKIPKEFVFRKELPKSPVGKILRKELRKES"
     gene            complement(2905..>3325)
                     /locus_tag="PLY93_10180"
     CDS             complement(2905..>3325)
                     /locus_tag="PLY93_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRP69886.1"
                     /translation="TQWAKIGRHMSAGSVAQARPDHDVVILYGDGSAGYSIVEFDSFV
                     RNRLPIAAIIGNDACWMQIYRDQKTLLGDDVACMLNYSPYDQIAKTFGGAGQTISKPA
                     QIKTAVTALKKSLSQKKPYILNVLIGRSEFRKGSISI"
BASE COUNT          761 a          760 c          875 g          909 t
ORIGIN      
        1 accccaaatg atttgctcct ttacgtcggt tgcgtcgatg gcgaacaaga cagttcaacc
       61 gtttggggtg tgcgtcgtgt aatcgccgaa tatgagcgtt taatccgtga aggaaaaaat
      121 accactgctc tcatgcaacc taaaaaagcg ttaccgcgcc gatatcggtg atacaaatcc
      181 gcgcaaaatc gagacggcgg atttgtgcgg cggtgatggc gtcgcgtttg ggttgatccc
      241 actcttcgtc gcctaaatcg tacgttcccc aatggatggc caaaagcttt ttagcgttta
      301 aatcttcaaa cgcgcggatc gattcgtcgg gggagacgtg cacttcgcgc ataaaccagc
      361 gggggaggta cgcgccaatc ggcatgagcg ttaagtcgat atcggaaaac tcgtgtgcaa
      421 tttcagtgaa gtggggtgag tacgcagtgt ctccagcaaa gtaaacattt tttttggggt
      481 cgttaaaaga aatcatcatc gaagtccaca gtgtttggtt gcggtcggtg gcgctcctgc
      541 ccgaaaaatg tttggaaggt aggcaagtga ttttgaattt ctccaaggcg acttcttgcc
      601 accagtcgag ttcataaatt ttatcgaagc caaaattttt gactaacggt tgaacgcgta
      661 agggtaaaaa aacaggggga ttgtcgcgtg cagctaaaaa cttgagcgtg tcgctttcgg
      721 tatggtcgta gtggttgtgc gagaggacaa tcgcgtcgag tttgggtnnn nnnnnnnnnn
      781 nnnnnnngac gttcgagttc aagcgcatgc cctgttaagc gtttaggacc cacaaattta
      841 acgggagata cgcgttcgcc aaaaacagga tcggtgataa tattgagtcc acctgcttgc
      901 aagagcaccg ttgcgtgtcc caaccaaatg gcgcgcgctt cgatgggtga aggaagaagc
      961 tctgcgtcgg tgggtggatg cacgcgtgca ggggtgccgc gttcacgcgg ttggattcct
     1021 ttgcgtaacg cccacgccgc cacgtcgagc gcacggtatt tcaaatcgcc gctttgtgca
     1081 tcgtacttac gaaattctga gtcggggtgt ggcccccaga tattacgaaa tcgctcgccg
     1141 ttgtggtgtt cggtggtgta ttgcggtagg ttcttgatcg cttgtaacag gcgtgtcatt
     1201 aggactaact ttctttacgc aactctttgc gtaaaatttt acccacaggg cttttgggta
     1261 attccttacg aaatacaaat tcctttggga ttttgtattt aatgagtttg tcgattaaaa
     1321 aattcttaag ttcatcagcg ctgacactca tgccttcttt gagtgcgata aatgcacgtc
     1381 ccacttcacc caagtggtcg tctttcgtac cgacaacgca cgcttctaaa acagcggggt
     1441 gtttgtagag cacatcttcg acctcggctg ggtacacgtt gtaaccgccg gaaataatga
     1501 ggtctttttt acggtcgacg atagcgaaat aaccgtcttc gtccatgcgc ccaatgtcgc
     1561 cggtgtatag ccagccgttt ttgattgtag cgcgggtggc ttcttcgttt tgccaatagc
     1621 ccaacatgag gtctgggctg tgtaaaatga tttcaccgtc tttaccgggg ggtaatagtt
     1681 cgccggtggt gtcgtcaaca atggccatga gagtgtctgg ccaagggata ccaatcgatt
     1741 tttctttttt aaggccgtgg taaggattgg cgcatttcgc ggtaactgac tcggtgagac
     1801 catacccttc gatgagagtg ccgccggttt tttctttgaa cgctacttta agcgcttcgg
     1861 gcaccggtgc cgcgcccaca aaacaaccgc gtagttttgc atttttcatt ttagcaaaat
     1921 ttttgtggtt gagcatcgcg ttgtacattg tgggaacgcc cgcgtaaacc gtgatgccgt
     1981 ctttttcaaa atgtttgatc gcttgccctg catcccagcg cggcatgagc accatggtgc
     2041 ctcccgagct taaactgacg ttgaggccaa cgctcatgcc aaagccatgg aaaaaaggca
     2101 atacgcaaag gcccacgtcg tcaagagtta aatcgcccca gtttttggcc tgcgtcatgt
     2161 tgacgaccat tgcgtagtgc gaaagcacaa cccctttgga aacaccggta gtgccgccgg
     2221 tataaataat caccgctggt tcgctgtttt taacaacaat tttggttgga gttttatcgt
     2281 taaattcttt caatactttt ttgtatttca ccgatgctgt ccgcttgccg tcgggaaggg
     2341 ggattttcag cgttgccttt ttaagtcgaa agaaaaagcg ttttactgcg gatagatagt
     2401 cggcgacatc gcacaccacg gtgggcgtcg tggtgtcttt ttggagtaga tcagaaagct
     2461 tactgtggag gaggtcaaga tatacaatga ccttcgcgcc ggaatttttg aatttgtatt
     2521 ctgcttcgct ggcggtgtct aaaggacttg caggtaccac aatggcgccg cgtttcaagg
     2581 ctgcgtagta cgcgacaata aaatggggac tattgggcag catgagaaaa acacgatcgc
     2641 ctttgccgat gcccttggct tctaaaaagg cagcgaatgc ggttgctgac ttctcaattt
     2701 ctgcatagca tattgactta cctaaaaatt taacaccgac gcgcgtggcg tgttctttgg
     2761 cgctttcttc aaaaagttcg ggcaaacttt tagacgagta ctccatcgtt tcggggacgt
     2821 gtttatcgta atgcttcacc catgggcgtg ggatggattt aggatcgggt ttaattgaca
     2881 taggtactcc ttaaaaattg ctccttaaat cgagatagaa cctttccgaa attcgctgcg
     2941 gccaatcaag acgttcagaa tatacggctt tttttgcgaa aggctttttt tgagcgcagt
     3001 tacagcggtt ttaatttgtg cgggttttga gattgtttgt cccgcaccgc caaaagtctt
     3061 tgcaatttgg tcgtacggcg agtagttgag catacatgcc acgtcgtcgc ccaagagcgt
     3121 tttttggtcg cgataaattt gcatccagca ggcatcgttg ccgatgatcg ctgctatagg
     3181 gaggcggtta cgcacaaacg aatcgaattc gacaatgctg taccctgccg aaccgtcgcc
     3241 atagagtatc accacgtcgt ggtcggggcg cgcctgcgcg acgctgccgg cggacatgtg
     3301 cctgccgatc ttcgcccatt gcgtg
//
LOCUS       DAOMPD010000300         3298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11803_length_3298_cov_2.658649, whole genome shotgun sequence.
ACCESSION   DAOMPD010000300 DAOMPD010000000
VERSION     DAOMPD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11803_length_3298_cov_2.658649"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>221)
                     /locus_tag="PLY93_10185"
     CDS             complement(<1..>221)
                     /locus_tag="PLY93_10185"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRP69887.1"
                     /translation="ATFYDPKLCEHLTSDSAARLGGATLAYAIESQLSPKANPISSAL
                     ALRAIELIRRNIPQLYKDGKNEKLITTML"
     assembly_gap    222..239
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<240..740)
                     /locus_tag="PLY93_10190"
     CDS             complement(<240..740)
                     /locus_tag="PLY93_10190"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRP69888.1"
                     /translation="MQGISWAQFSVPTRIQFENDSLFKVGNYVKQFGSRILLLNLKSE
                     NKNPSELTVLKNGLSKHSDGVIVYDDLTEDPNSDQVDSAAYFTKKSHADVIVAYGSLE
                     TFATAKLVALLVTNAVFASDLLNGRAQIKNPPLPLVTIPVEPSLGEELAPSFTIRDAA
                     DGHKKYF"
     gene            complement(839..1141)
                     /locus_tag="PLY93_10195"
     CDS             complement(839..1141)
                     /locus_tag="PLY93_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRP69889.1"
                     /translation="MNIDKINNAAHIHNVANKNNAKAPNSPSVGMGQDSVSISQAALQ
                     ARETALVEKAMASASDVRLDRVREVKEKLARGDYDNLSNEMLNQIATRLTTPIKNS"
     gene            complement(1217..1432)
                     /locus_tag="PLY93_10200"
     CDS             complement(1217..1432)
                     /locus_tag="PLY93_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69890.1"
                     /translation="MPIAPVKNAPKLAITAAKTKKLIDMNLKIKLDVNPTQYVIEDRE
                     ERINKKMLNAFALFADKIERGIKTFTG"
     gene            1964..3088
                     /locus_tag="PLY93_10205"
     CDS             1964..3088
                     /locus_tag="PLY93_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69891.1"
                     /translation="MKKIVIMATVLGMVYSSSVFAGPFTISNVTCTDIGASTLCSTSG
                     VMDGVRTKLKQIETDINKGLPPVDGERYANGMANSAVLTSKGLGVDYASNIKLFTIGG
                     SAGIAIDAGANGLSGMSNGAATMSGLGGSFSGVFGLNLGVFSLPKLGPIDFKKMIVFV
                     NVGYVNVGSLVGKAMGEGGSVSGSLTTVGLNARYRVIPEVSLLPFGLFAWSGLDITSG
                     INYSSMKIQLTAEFSESLSNNLGGGVNMSGNYNGTLTFGPDLGILTVPVDVSSGIRVL
                     NIFRLYAGLGLDINLGYSEIAAKVPGKVSGSLSGGGNSSTVNADAGLDLSAKGAADYL
                     AMRFFGGLQLDFSVLNIFVQYNTAITSGTHGVSMGARIYW"
BASE COUNT          774 a          662 c          863 g          981 t
ORIGIN      
        1 aaagcatcgt cgtaatgagt ttttcgttct taccgtcttt gtaaagctgc ggtatattcc
       61 ggcggatgag ttcaatcgcg cgtagcgcga gcgcgctgga gatggggttt gctttcggcg
      121 agagctggct ttcaatcgcg tacgcgaggg ttgctcctcc caaccgtgct gcgctgtcgg
      181 aagttaagtg ttcgcaaagc ttagggtcgt aaaacgttgc gnnnnnnnnn nnnnnnnnna
      241 aagtattttt tatggccgtc ggcggcgtcg cggatggtaa agctgggtgc aagctcttcg
      301 cccaacgaag gctcaactgg aatcgtgacg agtggaaggg gaggattttt aatctgagcg
      361 cgtccgttca ataaatctga agcaaagacg gcattggtca caagaagcgc cactaatttg
      421 gccgtggcaa atgtttccaa agaaccatac gccacaatca catccgcgtg tgatttttta
      481 gtgaagtatg ccgccgaatc aacttgatcc gaattggggt cttcggttaa atcgtcgtaa
      541 acgattacgc cgtcggaatg cttggataat ccgtttttga gaacggtgag ttcagagggg
      601 tttttatttt cacttttgag attgaggagt aaaatgcgtg aaccgaattg tttgacatag
      661 ttgcccactt tgaaaagcga atcgttctca aattgaattc gagtcggtac tgaaaattgt
      721 gcccagctaa tgccctgcat tattctttat tttcgacatg tatcaaagga agatttaccc
      781 ataaaaaagg cggcttatgc cgcctatgcg ctttgaaagg atggcgaata tcctttactt
      841 agctattttt gatcggtgtt gtcaaacggg ttgcaatttg attgagcatt tcgttggaca
      901 aattgtcgta atcaccgcgg gcgagttttt ctttgacttc acgcacacgg tctaaccgaa
      961 cgtcgctggc agacgccatc gctttttcga ctaaagccgt ttcacgcgct tggagcgccg
     1021 cttgtgaaat gctaacgcta tcttgtccca tgcccacaga gggggagttg ggcgctttag
     1081 cgttattctt gttggccaca ttgtggatgt gagccgcatt gttaattttg tctatattca
     1141 tagtcttcct tttttgccaa gctttcgttt ccgttagcct tgcggtgttc gcccgtatat
     1201 aagccttgct gagctttcac cctgtaaagg tctttatacc tctttctatt ttatcggcaa
     1261 acaaggcgaa agcattaagc atttttttat ttatacgttc ttcacgatct tcgattacat
     1321 actgcgtcgg attgacatcg agcttaattt ttaaattcat atcgatgagt tttttagtct
     1381 ttgccgctgt aatcgccagt ttaggcgcat tttttaccgg tgcaataggc acaacccgag
     1441 gcggttatgg tctaatatcg tcagttcgcc gccgttttgc atgctccgca gatattgact
     1501 gagatgcgct ttgaattcgg acacaggagc cattgttatc atgtgaccga ttaggtcata
     1561 ttggtcacaa tgtcaatgag atgattcctt tgcccgaggc aaagttaacg ctaaattcag
     1621 caatgggtca aagaccccat gcagaagact ctcatttccg tgcaaatatc tttgccgttt
     1681 tcacggaaaa cttgataaag gccaatgatt ggtgccccac cccggccccc tccccacaaa
     1741 tgtggagagg gggtgacgag gggcatttgc ctttggcaaa tggggggaag tatcaagatg
     1801 cgcctgcggc gcatcttgac ttcaaaaaac ccctctccat gtcaatgggg aggggtggcc
     1861 agcatcctgt gctggacggg gtggggctca ggctccatct cgttgtgctg tctaaaacgc
     1921 tgaaaaaggg ttgcaaattt aacgcaaagt gggaagaggg agcatgaaga agatagtaat
     1981 aatggctact gtgctgggga tggtgtacag tagtagcgta tttgccggtc cgtttacaat
     2041 tagtaatgtc acctgtacgg acattggcgc ctcgacacta tgtagtacaa gtggtgttat
     2101 ggatggggtt cgtacaaagc tgaagcaaat agaaacagat attaacaaag gcttgccgcc
     2161 agttgatggt gaacgttatg ctaatggcat ggcgaactcg gcagtgttaa cgtccaaagg
     2221 tttaggggtc gattacgcca gtaacattaa actctttact attggcggta gtgcaggtat
     2281 tgctattgat gcgggtgcaa atggtctctc aggtatgagt aacggcgcag ctaccatgtc
     2341 gggtttagga ggctcatttt caggggtgtt tggtcttaat cttggagtgt tttccttgcc
     2401 taagttagga cctattgatt tcaagaaaat gattgtcttt gtcaacgttg gatatgtcaa
     2461 tgtgggcagt cttgtgggga aagctatggg tgaaggtggc tctgtttcag gtagtctcac
     2521 tacggtgggt ttgaatgccc gttatcgggt catcccagag gtctctttat taccgtttgg
     2581 tttgtttgcg tggagtggtt tagacattac ttcgggtatt aactatagta gtatgaaaat
     2641 ccaactcaca gcagaattct cagaatcgct gagtaacaat cttggaggtg gtgtaaacat
     2701 gtctggtaac tataacggga cattaacttt tggccctgat cttggtattt taacggttcc
     2761 tgtggatgtg tcatcaggta tccgtgtatt aaatattttt cggttatacg cgggcttagg
     2821 gttagatatt aaccttggat attctgagat tgctgctaaa gtaccgggaa aagtttcggg
     2881 tagccttagt ggaggtggta atagtagcac ggttaatgcc gatgcaggac tcgatttaag
     2941 cgctaaaggc gctgctgact atttggcgat gcgctttttt ggtggccttc aactggattt
     3001 tagtgtattg aatatttttg tgcagtataa tactgcgatt acctctggta cacacggtgt
     3061 ctcaatgggt gcaaggatat attggtaaaa ttgtcctctc acgcgcgggg taaaaacgct
     3121 agccccgctt gatgctaccg ccgcatcgat gagcgatgcg gtgcgtgttg agtttttggg
     3181 agcggcgggt tttgttaccg gttcgcgtac cctcctcgaa tacaaaaatt ttagggtgta
     3241 tgtcgattgc ggtctttacc aagggcccaa gtatgtcgaa gaacgtaact acgcaccg
//
LOCUS       DAOMPD010000301         3286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11871_length_3286_cov_3.351284, whole genome shotgun sequence.
ACCESSION   DAOMPD010000301 DAOMPD010000000
VERSION     DAOMPD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11871_length_3286_cov_3.351284"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="eno"
                     /locus_tag="PLY93_10210"
     CDS             <1..1248
                     /gene="eno"
                     /locus_tag="PLY93_10210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRP69892.1"
                     /translation="VEVEVTLESGDTGRAIVPSGASTGSREALELRDGSLPDATAGDK
                     ARYGGKGVLKAVSNVNELINPHLVGVDALAQREVDGILLQLDGTENKAKLGANATLAV
                     SMATAHAAARYLDLPLFRYLGSPFSRTLPVPMMNVINGGAHADNNLDFQEYMIVPVAF
                     SSFRECLRQGAEIFHALKKVLHDKSYNTAVGDEGGYAPNVKSNREGLDLLMAAIEKAG
                     YKPGVDTFIAMDAASSEFYSNGKYKLDGDGGKELDASGLIELYADYCAKYPIVSLEDG
                     MAEQDWDGWKKLTEKLGSKIQLVGDDLFVTNKKILAEGIEKGIANSILLKVNQIGTLT
                     ETFETMQLAVKNGYTNVASHRSGESEDTTIADLAVAAETGQIKTGSMSRTDRIAKYNQ
                     LLRIEEMLGNDAFYPGIKAFKNH"
     gene            1248..1808
                     /locus_tag="PLY93_10215"
     CDS             1248..1808
                     /locus_tag="PLY93_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69893.1"
                     /translation="MQKLREIAQAVIAHRAFIPALIALAALAIGALLAVEHTRNILKI
                     RGPYLIVLTAIILFPLFAFRFFDPHEKARPISIFFVLSGVYFIYMTSPDIVEYFRMLL
                     FERMPPGTTRQLIFGFTAYISLAGALHYLLKPTNNENTSETTTKTHILDVPIIKFIIY
                     AVLHIIYLNLFVTIAREKFGITTMFL"
     gene            complement(1789..2463)
                     /gene="trmD"
                     /locus_tag="PLY93_10220"
     CDS             complement(1789..2463)
                     /gene="trmD"
                     /locus_tag="PLY93_10220"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRP69894.1"
                     /translation="MILHVVTLFPEFFESPLKASLLGKAIQSGILNVRYVNPRDFSEN
                     KYKKVDDHPYGGGAGMVMMAEPIARAVEHIKKEVPETRVALLAARGKLWNSIAIDEYA
                     KLPALTLICGHYEGVDERVAEHVADESIRIGNFVLSGGEVAALTIIDSLARKLPGFMG
                     NAHSLEHESFDDGASFEHAQFTRPEVWRNHKVPEVLLSGNHTRIAAWRHEEAQKFNDK
                     ITKTSS"
     gene            complement(2460..2963)
                     /locus_tag="PLY93_10225"
     CDS             complement(2460..2963)
                     /locus_tag="PLY93_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP family protein"
                     /protein_id="HRP69895.1"
                     /translation="MLRKALLFSITTFFAFAAKTNTNSFNQDMLKRHNHWRTKHGVPA
                     LEWNADVAKVAQDWANTIARADNMRHRQPNKYGENIFWISGRTPTGNDVVDAWYNEIK
                     DYNYNRPGFSMKVGHFTQVVWKDTTQIGCGSARSATGGIYVVCNYNPPGNYSGRFPAN
                     VLRPRTR"
BASE COUNT          809 a          792 c          816 g          869 t
ORIGIN      
        1 gtggaagtgg aagtgacttt agaaagtggc gatactggcc gcgcgattgt gccctcggga
       61 gcgtccaccg gcagccgcga agcactcgaa ctgcgcgacg ggtctctccc agatgccacg
      121 gcgggcgaca aagcgcgcta tggcgggaaa ggggtattga aagcggtttc taacgtcaac
      181 gaacttatca acccgcacct tgtcggtgtc gatgccctag cgcaacgcga ggtggatggt
      241 attttattgc aactcgatgg caccgagaat aaggctaaac tgggtgccaa tgcaactttg
      301 gcagtttcaa tggccactgc gcacgctgcc gctcgttatt tagatttacc tcttttccgt
      361 tatttaggtt cgccgttttc acgtacactc cctgtgccga tgatgaacgt catcaatggt
      421 ggcgcgcatg cggacaacaa tcttgatttt caagaataca tgattgtgcc ggtggcattt
      481 tcttctttcc gcgaatgctt gcgtcagggg gcagaaattt tccacgcgct caaaaaagtg
      541 ctgcacgaca aaagctacaa caccgccgtt ggtgacgagg gggggtatgc acccaatgtg
      601 aaatccaacc gcgagggtct tgaccttctt atggcggcga ttgaaaaagc gggctacaaa
      661 cccggcgtcg atacgtttat agcaatggac gccgcttcga gtgagtttta ctctaacggt
      721 aaatataaac tcgacggcga tggcggtaaa gaactcgacg caagtggcct tattgagctc
      781 tacgcagact attgtgctaa gtaccccatc gtgtcgctcg aagacggcat ggccgaacaa
      841 gactgggatg gctggaaaaa actgacggaa aaattgggtt caaaaatcca actcgtgggc
      901 gacgaccttt tcgtgacaaa taaaaaaatt ctcgctgaag gcatcgaaaa gggtattgcg
      961 aactccatcc tcctcaaagt aaaccaaatc ggtacgctca ccgaaacttt tgaaacgatg
     1021 cagctcgccg tcaaaaacgg ctataccaac gtggcgtcgc accgctcggg cgaatccgaa
     1081 gacaccacga ttgccgacct cgccgtcgcc gctgaaacgg gtcaaattaa aaccggctcg
     1141 atgagccgca ccgaccgcat cgcaaagtat aaccaactct tgcgcattga agagatgctt
     1201 ggtaacgatg cattctaccc tggcattaag gcgttcaaaa accactaatg caaaaactgc
     1261 gcgaaatagc ccaagctgtt attgcgcacc gtgcgtttat accagctttg attgcactcg
     1321 ctgcgctcgc tataggggcg ttgcttgcgg tcgaacatac acgcaatata cttaaaattc
     1381 gcggccctta tcttatcgta ttaacggcga ttattctatt ccctctgttc gcgtttcgtt
     1441 tttttgaccc ccatgagaag gcgcgcccca tttctatttt ctttgtgctt tcaggggttt
     1501 attttattta tatgacttca cccgatatcg tcgagtactt tcgaatgtta ctttttgaac
     1561 ggatgccccc cggaacaacg cgtcagctca tctttgggtt tactgcgtac ataagccttg
     1621 ccggtgcgct acactattta cttaagccca caaataatga gaatacaagt gaaacgacga
     1681 ctaaaactca tattttggat gtacccatca taaagtttat tatctatgca gtgttgcata
     1741 ttatttactt aaatcttttt gtcaccattg cgcgagaaaa attcggtatc acgacgatgt
     1801 ttttgtaatc ttgtcgttga atttttgcgc ttcttcgtgc cgccatgcag cgatgcgcgt
     1861 gtgatttccc gataagagta cctcaggcac tttatgattg cgccacacct cggggcgggt
     1921 gaattgcgcg tgctcaaacg acgcgccatc gtcgaatgat tcatgttcga gcgaatgggc
     1981 gttccccata aagcccggga gtttgcgcgc cagtgaatcg atgattgtca atgcagcgac
     2041 ctctcctccc gagagtacga aattacctat gcgtatgctt tcatcggcga cgtgttcggc
     2101 gacgcgctcg tcgacgcctt cgtagtggcc gcagatgagg gtgagcgcgg gcagttttgc
     2161 atattcgtcg atagcgatgg aattccatag cttaccgcgt gccgctaaga gtgccactcg
     2221 tgtctcgggt acctcttttt taatatgttc cactgcacgg gcaattggtt ctgccatcat
     2281 caccataccc gcacccccgc catatggatg gtcatccacc tttttatatt tattctcgct
     2341 aaaatcgcgg gggttgacgt agcggacgtt gagaatgcct gattggatgg ctttccccaa
     2401 aagcgacgct ttgagcgggc tttcaaaaaa ctcggggaaa agagtaacga cgtggagtat
     2461 caccgcgtgc gcggtctaag cacgtttgca ggaaaacggc cagagtaatt accgggtgga
     2521 ttataattgc aaaccacgta aataccacct gttgcagagc gcgcggaacc gcaacctatc
     2581 tgggtggtgt ccttccacac aacttgggtg aagtggccga ctttcatact aaagcccggg
     2641 cggttgtagt tatagtcttt gatttcatta taccacgcat cgacgacatc atttcctgta
     2701 ggagtgcggc cagagatcca aaaaatattt tcaccgtact tgttgggttg gcggtgccgc
     2761 atattgtcgg cgcgcgcaat tgtgttcgcc cagtcttgtg caactttggc gacatcggcg
     2821 ttccactcga gagcgggtac accgtgcttt gtgcgccagt ggttgtggcg cttcaacata
     2881 tcttgattga atgaattggt gttggttttt gccgcaaaag caaaaaaagt agtaatagaa
     2941 aataataacg cttttctaag catattcttc tttactaaga cgtgttgttg ttgtaaatcg
     3001 ttttcttgtt tttttatata gggcggattc aaatttgaag tcggtcgaaa tcgcagcgtt
     3061 gcttaatttt tcagagatca ctattttacg gcaaattgcg agtcagggca gcattcggtg
     3121 ttccgcttac ttggcgcaag aacgttgtgc tcgtggtctt aacgaaaata ggagcgggct
     3181 tatccaacaa gacttcccca aaaagaaggg ggggtagact tcttgcattg tggattatgt
     3241 cacattaggg gataactatc cacacgaatg atttctgcaa aaaaaa
//
LOCUS       DAOMPD010000302         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11943_length_3271_cov_2.173818, whole genome shotgun sequence.
ACCESSION   DAOMPD010000302 DAOMPD010000000
VERSION     DAOMPD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11943_length_3271_cov_2.173818"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PLY93_10230"
     CDS             <1..1432
                     /locus_tag="PLY93_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRP69896.1"
                     /translation="NSDFFPWAKPTPANYFRATFWTGFGGPELGPFKIYWNDLSLRFE
                     KRAPQIAVRETYDNESKDIYISGFTDERYDFSLYDEMQIRLLPNYFDLNLGFAFGKSW
                     DNDNTIRPDDYNSTRYSIVIRPVWYITEHLHYLLELVYAREKSTIGNRYREHFNSIKS
                     NTGGMSDTEGLEWGDTDTKYTYQIKTGFTINPAGRGIYSRPVIRLLYGVQHSNVQAAF
                     GNSFEESLNRRNQFNLNRDIHWHHMVRVEFEHWFSVSGWEDGYTPPLAQQTQRLSQVH
                     KGVPVDPNKPKPYDIYTALWGGYLLPFGPLTTALADRLVNEPDGCLGLNSRQCVINYT
                     LGGVSGGAHVRVRSHTLEYTAGLWYLPVYSAAISRTTGDVSTNVAFFVASAGMRYFPE
                     FIPSLKGVFVGAELGYGIPMIENSVAGDARDLNLGTYFVAMLSIGYQYRLSVHFSVEA
                     GTQFVFMPTTYVMLGATPYMAANYHL"
     gene            1429..3018
                     /locus_tag="PLY93_10235"
     CDS             1429..3018
                     /locus_tag="PLY93_10235"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRP69897.1"
                     /translation="MNIVEISSECSGLAQRGGLGSVIWGLIDAFSGMGHSVKLIIPYY
                     AEIQYGVRMYTALEIFFGGEKIPVSVFTTKHGGADVYLIHSDRFFRGAYADVYIDSGK
                     LGRGYFEDDAQRFAFFSLAAAHLLLHLHQQNKIDVLHAHDWHSGLVLLLTRIHPIFEK
                     LQSVREIFTIHNLEYQGTRPLYAMGELRAFFDWYTDLAYMGEKILDPYLDPHADIACI
                     NPMRAAINTATVVTTVSPSYAEEICRPDNSVADFYGGRGLEKDLASLRSEDRLCGITN
                     GIDQTFYTPEKLSIPYSATNFIKGRKENRAALFDDFSRIIEGMLKTQSLLNRCEKALI
                     EKYQKITRNEFLSKPLFVAVTRLTGQKIRQFFEVVHTKTVLEHCAHLNAHFIFLGKGD
                     LLQTLQEQTQSAHNIFLFGGFDDALEMRLYASADAMLMPSEFEPCGTSQMKAMRYGSL
                     PIAAAVGGLKDTIQNGDNGFLYDGASRHEKATEFLTTIRYVAELTHKNAPLFHEMQRT
                     AMATDFSWTSAAAEYMRVMVG"
BASE COUNT          809 a          788 c          782 g          892 t
ORIGIN      
        1 taactccgat tttttcccgt gggcaaaacc tacgcctgcc aattatttcc gcgcgacgtt
       61 ttggacgggc tttggtggtc ctgagttggg gccattcaag atttattgga atgatttgag
      121 tctacgtttt gaaaagcgcg caccccaaat tgccgtgcgt gaaacctacg acaatgaaag
      181 caaagatatt tatatttctg gtttcaccga cgagcgctac gattttagtt tatacgatga
      241 aatgcaaatt cgacttttgc cgaattattt tgatttgaat ttaggttttg ctttcggcaa
      301 aagttgggac aacgataaca caatacgtcc cgacgattat aatagcactc gctattcaat
      361 agtcattcgt ccggtgtggt acatcaccga gcatttgcat tacttgttag agttggtgta
      421 cgcacgcgaa aaatcgacca tcggtaatcg ctaccgcgag cactttaact ccattaaaag
      481 caacaccggc ggcatgtcag acaccgaggg tctcgaatgg ggcgacaccg acaccaagta
      541 cacgtaccaa attaaaaccg gttttacgat taaccccgct gggcgtggga tttattcgcg
      601 gcctgtgatc cgtcttcttt atggcgttca acactcgaat gtgcaagcgg cgttcggcaa
      661 ttcgtttgag gagagcctca accgccgcaa ccaattcaat ctaaaccgcg atatccactg
      721 gcaccacatg gtgcgtgtgg aatttgagca ttggttctcg gtgagcggtt gggaggacgg
      781 gtacactccc cctttggcgc agcaaactca acgcctctcg caagtacata aaggtgtgcc
      841 cgtcgatcct aataaaccta aaccgtacga catctacact gcgctgtggg gtgggtatct
      901 cctacccttt gggccactca ctaccgccct tgcggataga ctggtcaacg agcccgacgg
      961 ttgtttgggc ttaaatagcc ggcaatgcgt tattaactat actttgggcg gcgtctcggg
     1021 cggtgcgcat gtacgcgtgc gttcgcatac gttggagtac accgctggcc tttggtattt
     1081 acccgtgtac agcgctgcaa tcagccgcac tacgggggat gtctcgacga acgtggcgtt
     1141 ctttgttgcg agtgcaggta tgcgttactt tcctgaattt atcccctctc ttaaaggcgt
     1201 cttcgtcggc gccgagttgg ggtatggcat accgatgatt gaaaacagtg tcgccggcga
     1261 cgcgcgcgac ctcaaccttg gtacatactt cgtcgccatg ctgtccatag gttatcaata
     1321 ccggttgagc gttcacttta gtgttgaagc cggcacacag tttgttttca tgccgacaac
     1381 gtatgtcatg ctgggagcga ctccttacat ggcggcgaac tatcacttat gaacattgta
     1441 gaaattagta gtgaatgctc gggactggcg cagcgcggcg gcttgggtag cgttatctgg
     1501 ggtcttatcg acgcgttcag cggcatggga cactcggtaa aactcattat cccctattac
     1561 gcagaaatcc aatacggtgt gcggatgtac actgcgctcg agatattttt cggtggagag
     1621 aaaatccccg tctctgtttt caccacaaaa cacggtggtg cagacgtcta tttaatccac
     1681 agtgatcgat tctttcgtgg tgcatacgcc gacgtgtaca tcgacagcgg taaattgggg
     1741 cgtggctatt ttgaagacga cgcgcaacgt tttgcatttt tttctttagc ggcggcgcac
     1801 ttgcttttac atctacatca acaaaataaa atcgatgtgt tgcacgcgca cgattggcac
     1861 agcggtttag tattgttact cacgcgtatc caccctattt ttgaaaagct gcaatctgtt
     1921 cgagaaattt ttacaataca taatttagaa tatcaaggta cgcgaccact ctatgcaatg
     1981 ggagagctac gcgcattttt tgattggtac accgatttag cttatatggg tgaaaaaatt
     2041 ctcgatccgt atcttgatcc ccacgccgac attgcgtgca tcaatccaat gcgtgcggcc
     2101 atcaacacag ccactgtggt gacgacggtt tcgccatcgt atgcagagga aatttgcaga
     2161 ccagacaaca gcgttgccga tttttacggt ggtcggggtt tagaaaaaga tttagcttct
     2221 ttgcgctcag aagatcgcct ttgcgggatc accaacggta tcgatcaaac attttatacg
     2281 cccgaaaaat tatcgattcc ttattccgcc acaaatttta taaaggggcg caaagaaaat
     2341 cgcgctgcgc tcttcgatga tttttctcgc atcatcgaag ggatgctcaa aacacaatcg
     2401 cttttgaatc gttgtgaaaa agcgcttatt gaaaaatacc aaaaaataac gcgtaatgag
     2461 tttctctcaa aaccactttt tgtggcagtc acgcgtttga cagggcagaa aatccggcag
     2521 tttttcgagg tcgttcacac caaaacggtt ttagaacatt gcgcgcatct taacgcgcat
     2581 tttatttttt tgggtaaagg cgatcttttg caaacgctac aagagcaaac gcaaagcgct
     2641 cacaatattt ttctttttgg tgggtttgac gatgcgctcg agatgcgtct ctacgcctct
     2701 gccgatgcga tgctcatgcc gtctgaattt gagccgtgcg gtaccagcca aatgaaagcg
     2761 atgcgttacg gttctttacc tattgccgct gcggtaggcg gtctcaaaga caccatccag
     2821 aacggcgata atggtttttt atacgacggt gcttcgcgcc acgaaaaggc caccgagttt
     2881 ttaaccacaa tccgttatgt cgcagaactc acccataaga acgctccgct cttccacgaa
     2941 atgcaaagaa ccgcaatggc caccgatttt tcgtggacta gcgcagcggc agaatatatg
     3001 cgggtgatgg tgggataact atctcaacct cttatgtcgt attaggtata attatttata
     3061 tgagcaattt ttggtgaaac gtacgctttt tttctaagat actcgcgtta aagttctcaa
     3121 cgtaccgcga ccttgttagg gtaaattaag tattcttgag ggagcacccc ccaagaatac
     3181 atcgaggcta aattgcttcg ccgccgcgcg taccgtcgct gatacgcacc acttcatcga
     3241 gtggcagtac gaaaattttt ccgtcgccaa t
//
LOCUS       DAOMPD010000303         3263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11978_length_3263_cov_2.020262, whole genome shotgun sequence.
ACCESSION   DAOMPD010000303 DAOMPD010000000
VERSION     DAOMPD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11978_length_3263_cov_2.020262"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLY93_10240"
     CDS             complement(<1..564)
                     /locus_tag="PLY93_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69898.1"
                     /translation="MPKLIAVVYKNSALENYSTKQIRRHRELNPHLSAQMVQAHEEHA
                     ASLDALHRIVDTLQLKTEFFERDRLRQNLDNFALVISLGGDGTFIHASHYIKNVSSNQ
                     TLLVGINSSPKHSVGHYCRFHLLAKKNENQFAAMLAAFFNEKNPSWKENFLLRMRVTV
                     QGQEVAYPILNDVLFCEKEAGSTSRYTL"
     gene            complement(751..2160)
                     /locus_tag="PLY93_10245"
     CDS             complement(751..2160)
                     /locus_tag="PLY93_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRP69899.1"
                     /translation="MKTRTVQIMDTTLRDGEQTYNVAFSASEKLQVARLLLEKANVNF
                     VEVASARVSLGELESVKQITAWAKKTGGQKLLERIEILGFVDEKRSVAWMLEAGVLTL
                     NMLTKGSLHHLQNQLRKTPKEHFDDIQKTIEYAIKEGRKVNVYLEDWSNGYLNSRAYM
                     YEYLDFIATQPVGRIMLPDTLGVLYPKDVYAAISEITERYPKNRFDFHPHNDYGLGTA
                     NVLEAIRAGAHGVHTTVNCLGERTGNASLAEVVAGIHDHLGLKTTVNEKSLFNLSRTV
                     ETFSGKRLAANTPIVGEDVFTQTAGIHADGDKKGGLYESRLLPARFGRDRRYALGKLA
                     GKASLDQNLKTLGITLDDAERAQVLKRIIELGDQKKQISVDDLPFIIGETLKRPEEKI
                     VKIEQVTVTSGLHVRPMCSLTVSYKDKNTQVSGQGEGGYDAFMVALTSWAKANKIKMP
                     RLIDYEVRIPPGGQTSALV"
     gene            2263..>3263
                     /locus_tag="PLY93_10250"
     CDS             2263..>3263
                     /locus_tag="PLY93_10250"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRP69900.1"
                     /translation="MNDIIQKKKLGNKLSQAEIKRFIDGVTSGKIPDYQTSALLMAIC
                     WRGMDVEERTYLTQAMADSGKKLNFRSMDGLKVDKHSTGGVGDKTSLMLAPWLACVER
                     KVPMISGRGLGFTGGTLDKLAAIPGYRIDFTEKELSQFLKKNGCFVIGQTAAIAPADK
                     KIYALRDVTATVDEISLITASILSKKLTEDLDVLVMDVKCGSGAFMQSLKDATALATS
                     IANTANKAGVRTVSLITAMNFPLGRYTGNTAETAEAYTFCRADSAYHRIAEKLYIIKE
                     KLTDAEKLLQSLVEITVELAILLLDPDAIKNKTKRNTALVDLFGQWQSGALEKKFIE"
BASE COUNT          812 a          819 c          774 g          858 t
ORIGIN      
        1 caacgtatag cgcgaagtgg agcccgcttc cttttcacaa aaaagcacgt cgtttaaaat
       61 gggatacgcc acctcttggc cttgcacggt aacgcgcatc cgcagaagga aattttcttt
      121 ccaagacgga tttttttcat tgaagaatgc agcgagcata gcggcaaact gattctcgtt
      181 tttcttggcg agcaaatgaa aacggcagta atgccccaca ctgtgcttgg gtgacgagtt
      241 aatccccaca aggagcgtct ggtttgagga tacgttcttt atataatgcg acgcgtgaat
      301 aaaagtaccg tcgcctccca acgaaatcac aagcgcgaag ttgtccaagt tttggcgtaa
      361 ccgatcgcgc tcaaaaaatt ctgtcttgag ttggagcgta tccacaatgc ggtggagcgc
      421 atcgagcgac gccgcgtgtt cttcgtgcgc ttgaaccatc tgcgcgctca aatgcggatt
      481 aagttcccga tgccgacgga tttgtttcgt gctataattt tccagagcag aatttttgta
      541 aacaacagca atgagtttag gcatacttat tttctaagaa tgatgttgag catccgctcg
      601 gtcgcacgaa ttgccgcaat cacttggtcg acatcgacgc cccgtgtggt gagaatacgc
      661 tcgtcttttt caatacccaa ctcaccgaca taaaacggct gtttttttgt ccttgtactg
      721 cctttttcct tttcgctctg ccaactgatt ttacaccaac gccgacgttt gaccgccggg
      781 aggaatccgc acttcgtaat cgatgaggcg tggcatttta attttgtttg ctttcgccca
      841 tgacgtcaac gccaccatga acgcatcgta accgccttcg ccttgtcccg acacttgcgt
      901 gtttttgtct ttgtacgaaa cggtgagcga gcacatcggg cgcacgtgca aaccggaggt
      961 cactgtgact tgctcgattt tcacaatttt ttcttcgggg cgcttgagtg tttcaccgat
     1021 gatgaacgga agatcgtcga cgctaatttg ctttttttga tcgccaagtt caataatgcg
     1081 tttcaaaact tgcgcacgct cagcgtcgtc caatgtaatt cccagcgttt tcaaattttg
     1141 atcaagcgat gctttgcccg ccagtttacc caatgcgtaa cggcggtcgc ggccaaagcg
     1201 tgcgggtaaa agtcggcttt cgtaaagtcc gcctttttta tcgccatcgg catggatgcc
     1261 agccgtttgc gtgaatacat cttcgccgac aatcggtgtg ttcgcggcca agcgcttgcc
     1321 cgaaaaagtt tcaacggtgc gcgataagtt aaaaagactt ttttcattca cagttgtctt
     1381 gagtcccaag tggtcatgga tgcccgccac cacttctgca agcgatgcgt tacccgtgcg
     1441 ttcacccaaa cagttcactg tagtgtgtac cccatgcgca ccggcacgga ttgcttcaag
     1501 tacgttggca gttcccagtc cgtagtcgtt gtgcggatgg aaatcgaaac gattcttggg
     1561 atagcgttcg gtgatttctg aaattgccgc atacacgtct ttgggataaa gcacgcccag
     1621 agtatccggg agcatgatgc gtcccaccgg ttgcgtggcg atgaaatcca aatactcgta
     1681 catatacgca cgcgagttta agtaaccgtt cgaccaatct tcgagataga cattgacttt
     1741 gcgcccttct ttgatagcgt attcgattgt tttttggatg tcgtcaaaat gttctttggg
     1801 cgtcttgcgc agttggtttt gcaaatggtg tagcgaacct ttggtaagca tgttgagtgt
     1861 aagcacgcct gcttcgagca tccacgcaac cgaacgcttt tcatcaacga agcccaaaat
     1921 ttcaatgcgc tccaaaagtt tttgaccgcc tgtttttttt gcccacgcgg tgatttgctt
     1981 tacgctttcg agctcaccca acgaaacacg cgccgaggcg acttcgacaa aattcacatt
     2041 ggctttttct aacaataacc gcgcgacttg tagcttctcc gacgccgaaa atgcgacgtt
     2101 gtacgtctgc tcgccatcac gcagcgtcgt atccataatt tgaacggtac gcgttttcat
     2161 ttgccctcat gaaaggtctt tatacacggt gaaaccaaaa aaggaactca ggcattcaaa
     2221 ctttacagtt gtcgtataga ctcaaacaat gaacatttca ttgtgaacga tatcatacaa
     2281 aaaaagaaac tgggtaataa actttctcaa gcagaaatca agcgctttat cgacggtgtt
     2341 acctctggca agattcccga ttaccaaacc agcgcactct tgatggccat ttgttggcgc
     2401 ggcatggatg tggaagagcg tacctacctc acgcaagcga tggcggatag cggtaagaaa
     2461 ctcaacttcc gctctatgga tggccttaaa gtcgacaaac actccaccgg cggcgtggga
     2521 gataaaactt cgctcatgct ggcgccgtgg ctcgcctgcg tcgagcgtaa agtgccaatg
     2581 atttcaggta gagggttggg ttttaccggt ggcactctgg ataaacttgc agccatcccc
     2641 ggataccgta tcgatttcac tgagaaagaa ctttctcaat ttttgaaaaa aaacggttgc
     2701 tttgtcattg ggcaaactgc agcgattgcc cccgccgata aaaaaatata tgcactgcgc
     2761 gacgtgacgg caacggtcga tgaaatttca ctcatcaccg cctcgatcct cagcaaaaaa
     2821 ctcaccgaag atttagatgt tttggtgatg gatgtcaaat gcggatcggg ggcgtttatg
     2881 caaagcttaa aagacgccac tgcgctcgct acttcaatcg ccaacacagc gaacaaagca
     2941 ggggtgcgca ccgtaagtct catcaccgcg atgaattttc cgctggggcg ttataccggt
     3001 aacaccgccg aaacggcgga agcgtacact ttttgccgtg cggacagcgc gtaccaccgc
     3061 attgccgaaa agctttatat tatcaaagaa aagctcactg acgcggagaa acttttgcaa
     3121 tcgttggttg aaattactgt cgagctggcg atcttactcc ttgaccctga cgcgataaaa
     3181 aataagacca agcgcaacac cgcgcttgtc gatctcttcg gtcagtggca aagcggcgcg
     3241 ctagagaaaa aatttatcga gtg
//
LOCUS       DAOMPD010000304         3261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_11987_length_3261_cov_2.415159, whole genome shotgun sequence.
ACCESSION   DAOMPD010000304 DAOMPD010000000
VERSION     DAOMPD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11987_length_3261_cov_2.415159"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLY93_10255"
     CDS             complement(<1..795)
                     /locus_tag="PLY93_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HRP69901.1"
                     /translation="MTSLTEREKLMSSVAASVDSGARRSAACAAINLQLRTLERWEKQ
                     TLGDKRLIIEKQPANKLTAAERSEVLRICCSDEFKDMTPNEIVPKMAETGRFIASEAS
                     FYRILKCEGMLRKSGAHKTPRRKKPTPLTASGPCQVWSWDITYLRSPVKGLYYYLYLV
                     QDVWSRAIVGWAIHETESAEHAAELMKKISTEMDIKNVKLHADNGGPMKGATMLATLQ
                     RLGVTPSFSRPSVSNDNPYSESLFKTLKYRASYPDTFASIEQVQQWV"
     assembly_gap    927..966
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<967..1298)
                     /locus_tag="PLY93_10260"
     CDS             complement(<967..1298)
                     /locus_tag="PLY93_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRP69902.1"
                     /translation="MEFKSEFKKEMVRKLLLPGGKGIVALSREIGESPQTLYNWRDKY
                     SYGSDTMSRKKRAGGLTSAERLKAVLEFARLPQSETGEWLRQKGLRSDHLKLWESELL
                     AQSEDRGY"
     assembly_gap    1539..1548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1549..2168
                     /locus_tag="PLY93_10265"
     CDS             <1549..2168
                     /locus_tag="PLY93_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69903.1"
                     /translation="TTALFADTHNAGRFGAEFSYGDTIGVLRRSNQRPAYVSVPTLAL
                     WYHFTDSIAVNLRAGLYMTTFVREFSYGICEYSICELPPTKNELTSYAFSIEIPFYIA
                     QLNAVRLYLAPSAGYNYMQTEMFYFSAASSVPEAQFKYLSEQYSVSATLGVQVALLEQ
                     LHILGRTTFGYIWTGQPPTSFINTPNQQFYLGFQSWSLGAIVYFN"
     gene            2234..2830
                     /locus_tag="PLY93_10270"
     CDS             2234..2830
                     /locus_tag="PLY93_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69904.1"
                     /translation="MLCLHTGVLSATVRGGRLGTEFSYGDTFGLFNAGLGSTYNGLPS
                     MAFWYHFTDWVAVNVRTGFYMTGGNRQDSYSSSKENYVSFGTSIEVPFYVARFERVHL
                     YLAPSFGYTFNWATSSSTDELVGSDKSKLLDEFFSTFAVFGVQIEVMDQLHIIGRTTL
                     GYIWGNVNQDYAYGSNRGRHLSYFGFQSWSLGAVVYFN"
     gene            2858..3103
                     /locus_tag="PLY93_10275"
     CDS             2858..3103
                     /locus_tag="PLY93_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69905.1"
                     /translation="MKKFFAIVVFLHLHSNQLSAVSLYAGGTLGSSYSTVGNGSVGAC
                     ISNACYYTGPKWDIFGRNVGRVYLADLYSIKVGFRFY"
BASE COUNT          733 a          768 c          744 g          966 t
ORIGIN      
        1 gacccattgc tgcacttgct cgatggatgc aaatgtgtca ggatagcttg cgcggtattt
       61 gagagttttg aataacgatt cagaataggg attgtcgttg gatacactgg gccgggaaaa
      121 tgaaggtgtc acacccagcc gctgcaaggt cgcgagcatc gtggcaccct tcataggtcc
      181 gccattgtcg gcgtgtagtt ttacgttttt tatatccatt tctgtgctga tttttttcat
      241 gagttctgct gcatgctccg cgctttcggt ttcatgaatt gcccaaccga cgatcgcgcg
      301 gctccagaca tcctgaacca aatagagata atagtacaga ccttttacgg ggctgcgcag
      361 atacgtgatg tcccagctcc agacttggca agggccggat gcagtaagtg gcgttggctt
      421 ttttcgccgt ggtgttttgt gtgcgccgga ttttctgagc atgccttcgc atttcaaaat
      481 ccggtaaaaa ctcgcttctg aagctataaa acgccctgtt tccgccattt tgggcacaat
      541 ttcattcggt gtcatatctt tgaattcgtc gctgcaacag atacgcagaa cttcgctgcg
      601 ctcggccgct gtcagtttat tcgcaggttg tttttcaata atcaatcgtt tatcgccaag
      661 cgtctgtttt tcccaacgct caagagttcg cagttgcagg ttgatcgctg cgcacgcggc
      721 actgcgccgc gccccgctat ctaccgaagc ggcgacgctg ctcatcagtt tttctctctc
      781 tgtcagcgaa gtcatagttc cttttcctct ggaaataaat ctaccgtttt tttttgcagg
      841 attatgattg cggaaagctc tgccagagct ttgtccttct tccgcaaatc cttttctagg
      901 tttttatttg cgccacgcaa caggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnntggt aacctctatc ttcgctctgc gccagtaact cgctttccca tagcttcagg
     1021 tgatcgctcc gtaagccttt ctgacgcagc cattcgccgg tttcactctg cggtaatctt
     1081 gcaaattcca gtactgcttt cagcctttcc gcagacgtta agccgccggc tctttttttt
     1141 cgagacatgg tatcactgcc gtaactgtac ttgtcgcgcc aattataaag agtctgagga
     1201 ctttccccga tctcccggga caacgccact attcccttgc cacctggcaa gagcagcttc
     1261 ctgaccattt ccttcttaaa ttcactcttg aattccattg tatcctccgt ttatttggag
     1321 gcgacaacta tcctgagatt ttccgatatg ggcaccttgt agatcgattt gtaatagata
     1381 tgaaagtaaa aagcttacat tcaattgatc ttgcccgatt aaagaacaat tcaagtaata
     1441 tgttgagcat tcccgaccaa ccaacaatat gtttttccgg cacgaggtta ccgtattagt
     1501 agagtgagtg tgcacccatg aaaaaactta ccctcatcnn nnnnnnnnac accaccgccc
     1561 tctttgcaga tacccacaac gccggacgtt tcggcgccga gttcagctat ggcgacacga
     1621 taggtgtttt gcgtaggtcg aaccaaagac cggcgtatgt cagcgttccg acactcgcgt
     1681 tgtggtatca ctttaccgat tctatcgctg taaacttgcg cgccggtttg tatatgacta
     1741 cgtttgttcg agagttctca tacggcattt gcgaatacag catttgcgaa ctgccgccga
     1801 caaaaaacga actgacgtcg tatgccttct ctatcgaaat tcctttttat atagcgcaac
     1861 tgaatgcggt tcgtttatat cttgcaccaa gtgcggggta caattatatg caaactgaaa
     1921 tgttttattt cagcgctgca agctccgtac ctgaagcgca gttcaaatat ttgagcgagc
     1981 agtatagcgt gtctgctaca ctcggtgtgc aggttgcgct tttagagcaa ctgcacatac
     2041 ttggtagaac gacgtttggt tatatttgga cggggcaacc gccaacttct tttattaata
     2101 cgccgaatca gcaattttat ttgggctttc aaagctggtc gttgggggcg atcgtttatt
     2161 ttaattgatt atggccgttt cgagattaca tccccgtttg attgctatac taagaagaag
     2221 catacttatc gctttgctat gcctacacac cggtgtgctg tctgccaccg taaggggcgg
     2281 acgcttgggc accgagttta gctatggcga cacgtttggc cttttcaatg ccgggcttgg
     2341 gtcgacttac aacggattgc ccagcatggc gttttggtac cacttcaccg actgggtcgc
     2401 cgtgaatgtg cgcaccggtt tttacatgac cggtggaaat cgtcaagact cttattctag
     2461 ctcgaaagag aactacgttt cttttggaac ttcgatcgaa gtaccttttt atgtagcccg
     2521 ttttgagcgc gtgcatttat accttgcacc cagctttggt tatacgttca attgggcgac
     2581 cagctcttca acagatgagc tggtcggtag cgacaaaagc aaacttctcg acgaattttt
     2641 tagtaccttt gccgtgttcg gcgtgcaaat cgaggtgatg gatcagttac acattatcgg
     2701 gcgcaccaca ttgggttata tttggggtaa cgttaatcaa gactatgcgt atggcagtaa
     2761 tcgcggccgc catctgtcgt attttggttt tcaaagctgg tcgttggggg cggtggttta
     2821 ttttaattga tatggtaatt aagaggttaa acttaaaatg aaaaaatttt tcgcaatagt
     2881 tgtttttcta cacctgcact ccaaccagct cagtgcagta tctctctatg ccggtggcac
     2941 gttgggtagt agctattcga ccgtgggcaa cggctcggtg ggggcttgta tcagcaatgc
     3001 ctgctattat accggcccta aatgggacat ctttggccgc aatgtgggca gagtgtactt
     3061 ggccgactta tactctatta aagtaggttt tcgattttat tgaggcgtaa aggtaaaatg
     3121 acactcaaag aaaatacttt ttaaccgcct tacgaaactt tcgcatagac ggcaatgaaa
     3181 acgaaggcga agtagaaaaa atcgtcacac gacgctgcaa ttcttttttg ctatttcaat
     3241 tcagcgccgg cttttttcca t
//
LOCUS       DAOMPD010000305         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12031_length_3252_cov_2.302784, whole genome shotgun sequence.
ACCESSION   DAOMPD010000305 DAOMPD010000000
VERSION     DAOMPD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12031_length_3252_cov_2.302784"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLY93_10280"
     CDS             complement(<1..1137)
                     /locus_tag="PLY93_10280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP69906.1"
                     /translation="MRIIDKLLLGIAALSIMLMVTIFFSDDSTLAAKNTISNKNQDKI
                     REYPVSLNNTIYRDHDDLKSADMDVAMEEYLDDLRAHPEKREEELRLIDDLSPEDAGI
                     LPPLNGTSGNNNSLQPTSNEKDTAPKIQKNNDGRVQIIQHKVKSGESIWRLAQRYNVP
                     VQTIVNANKIKEKSIIQPGDTLRIPDRAGIFHTIKKQESLKQIAKKYKINPDKIKKAN
                     GLTSTLLAKGDEIFLPGAKPLPEIRYVQQKVFAWPIKAKYRLTSPFGWRVHPITHHKA
                     FHSGIDIGAMMKTPIHAAADGVVAFAGNAGSYGNMVLLRHKNGLFTVYGHASKLIAKK
                     GQFIKRGQKIALVGSTGASTGPHLHFEVKSTEKHLSPFKALKKME"
     gene            1217..2218
                     /locus_tag="PLY93_10285"
     CDS             1217..2218
                     /locus_tag="PLY93_10285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP69907.1"
                     /translation="MIYILGAGIAGLTLAEVLHARNMDFQVFEKQASLGKEASGKNAG
                     MLRTYDTDTVLKPFTLKSLEYYRQNEPSFVERGMILKPWEIDYAETPEGEKFFSHNRF
                     KGFFLPANGTLEPLEVLDRLHKKVKNIALNAPVGLKVAAQRIAALKIAEEEIPLKEDD
                     KIVVAAGEGSVALAHETATPIELIAYRRTLYEFKNTIGHNGPIEWDEEASVYFRPSGG
                     GKTLTATAGDQIPVAQGTVDEDLEDDKAFQIIEREFPFLNRENLVSKRICRRLMPLDN
                     RPFIGRDTSLANLYWFTGLGGRGMSMSPGIAPYFADLLLNEKKILEFEPFLPGRVVV"
     gene            2280..>3252
                     /locus_tag="PLY93_10290"
     CDS             2280..>3252
                     /locus_tag="PLY93_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69908.1"
                     /translation="MFNKTALLILLSFFVSLSAENVAEKAKSKRAEALNQIIYLEIPL
                     RNFSSEKPELIKEYDEVKNKYSVALSFFFEDNFVESYKIFLEVQNSLEKLYEQLSLFY
                     IDRTNVVLAQSVKEATEIEVRYNRKAPFATQAIGKKREAGVRISQDKKETKLEQRLYD
                     PTDIHYLYDKKAMIDNIDHAYMLLGQAKLARQKSLDLEKWLEKGKPMPPTMKKERIEN
                     YKAVIDLCRQAKVNGIMVMQLNRIYDNYSLQTRFKDNYYMKEKRLDPVFDFAIPDEFV
                     KDASDSRNVAYDQRERIMIKGEDPTKVMNEDRKPVPKAKRFDGGASNG"
BASE COUNT          899 a          683 c          748 g          922 t
ORIGIN      
        1 ttccatcttt tttagagctt tgaacggact caaatgtttt tctgtcgatt ttacttcaaa
       61 gtgcaagtga ggaccagtcg atgcgccggt ggaacccacc agcgcaattt tttggccgcg
      121 ctttataaat tgtccttttt tagcgatgag tttagaagca tggccgtaga cggtgaataa
      181 tccatttttg tggcgcaata aaaccatgtt gccataacta cccgcattgc ctgcaaacgc
      241 cacgacgccg tcggcagcgg catggatggg ggttttcatc atcgcgccaa tgtcgatgcc
      301 ggaatggaat gctttatggt gcgttatggg gtgaacgcgc cagccaaaag gggatgtcaa
      361 acgatacttc gctttaatcg gccatgcaaa aactttttgt tgcacgtagc gaatttcagg
      421 taacggttta gctcccggca aaaagatttc atcgcctttc gctaacagcg ttgaggttaa
      481 tccgttcgcc tttttaattt tatcagggtt aattttatat tttttggcaa tttgttttag
      541 actttcttgt tttttaattg tatggaagat accggcgcga tcgggaatgc gcagcgtatc
      601 gccgggttga ataatcgatt tttctttgat tttgttggcg ttcacaatcg tttgcacagg
      661 cacgttatag cgttgagcaa gccgccaaat cgactctcca ctttttactt tatgctgaat
      721 aatctgaaca cgaccatcgt tatttttttg tatttttggg gcagtatcct tctcgttcga
      781 ggtgggttgg agagaattgt tattgccgct tgtaccattc aacggaggca atatacctgc
      841 gtcctcgggc gaaagatcgt cgatgaggcg taattcttct tcgcgctttt cggggtgtgc
      901 gcgtaagtcg tcgagatatt cttccatcgc gacatccatg tcggcgcttt tcaaatcgtc
      961 gtgatcgcgg taaatggtgt tgttgaggct aacgggatat tcgcgtattt tatcttgatt
     1021 tttatttgat attgtatttt ttgcagcgag tgttgaatcg tcgctaaaaa aaatagtcac
     1081 cataagcatt atagagagcg ccgcaatacc gagtaacaat ttatctatga tacgcacttt
     1141 tattattttc ggcacttaag ctatatagat ttgagtcttt tttacttttc gtgggtgttc
     1201 aagattaaaa taaggcgtga tttatatttt aggcgcaggc attgcgggac ttactcttgc
     1261 tgaagtcttg cacgcgcgca atatggattt tcaagttttt gaaaaacagg cgagtcttgg
     1321 caaagaggca tcgggtaaaa atgcgggcat gctccgcacg tacgataccg atacggtgct
     1381 taaaccgttt acgttaaaaa gccttgaata ctaccgtcaa aacgaacctt cgtttgtcga
     1441 acgcggtatg attctcaaac cgtgggaaat cgattacgcc gaaactcccg aaggagagaa
     1501 gtttttctcg cacaaccgct ttaagggctt ttttttgccc gccaacggta cgcttgagcc
     1561 actcgaagtg cttgaccggt tgcataaaaa agtaaaaaat atcgccttga atgcgccggt
     1621 ggggctgaaa gttgcagcgc aacgcatcgc ggctttaaaa atagcagaag aagaaatccc
     1681 acttaaagaa gacgataaaa tcgtcgttgc cgccggtgaa gggtcggtgg ctctcgcgca
     1741 cgaaacagcg acccccattg agcttattgc gtaccgccgc accctctatg agtttaaaaa
     1801 caccattgga cacaatggcc ccatcgagtg ggatgaagaa gcgagcgttt atttccgtcc
     1861 ctcgggtgga ggtaaaacac tcaccgccac cgcgggcgat caaataccgg ttgctcaagg
     1921 cacggtggac gaagacttgg aagacgataa agctttccaa attatcgaac gcgagtttcc
     1981 gtttttgaac cgcgagaatt tagtgagtaa acgtatttgc cgccgcctca tgccgctcga
     2041 caatcgtccc tttattggcc gcgacacatc gcttgccaat ctttactggt tcaccggctt
     2101 gggcgggcgc ggtatgtcga tgtcgccggg cattgcccca tactttgcag atttacttct
     2161 aaacgagaaa aagatcctcg aatttgagcc atttttaccc ggacgagtgg tggtgtaaaa
     2221 aaatatatct ttactttact tctttgggcc attttccgat attgaataag aggactctta
     2281 tgtttaacaa aaccgcactt ctcattctct tgtcattttt tgtgtcttta agcgcagaaa
     2341 atgtcgctga aaaggcaaag tctaagcgtg ccgaggcatt aaaccaaatt atttatttag
     2401 aaataccttt gcgcaatttt agctctgaaa aacccgagct aattaaagaa tacgacgagg
     2461 tcaaaaataa atactcggtg gcattgagtt ttttctttga agataatttt gtcgaatcat
     2521 ataaaatttt cttagaagta caaaattctc tggaaaagct ttatgaacaa ctttcacttt
     2581 tttatatcga ccgcactaat gttgttttag cgcaatcggt aaaagaagcc accgaaattg
     2641 aagtccgcta taacagaaaa gcccctttcg caacacaggc aatcggtaaa aagcgtgaag
     2701 caggcgtgcg cattagccaa gacaaaaaag aaactaagtt agagcaacgt ctttatgacc
     2761 ccaccgacat ccactatctg tacgataaaa aagcgatgat tgacaatatc gatcacgcgt
     2821 atatgttatt gggacaagcg aagcttgcgc gtcaaaaatc gctggattta gaaaagtggt
     2881 tggagaaggg gaaacccatg ccacccacca tgaaaaaaga acgcattgag aattacaagg
     2941 cggtgattga tctttgccgc caagcaaaag taaacggcat tatggtgatg caactgaatc
     3001 gtatttacga caattatagt ctgcagacac gcttcaaaga caactattac atgaaagaga
     3061 aacggttaga tcccgttttt gattttgcaa tccccgacga atttgtgaaa gatgctagcg
     3121 attcgcgtaa cgtcgcttat gatcaacgtg aaagaattat gattaaaggg gaagatccca
     3181 ctaaagtgat gaacgaggat cgtaaacctg tacctaaggc aaaacggttt gacggcggtg
     3241 caagtaacgg aa
//
LOCUS       DAOMPD010000306         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12034_length_3251_cov_4.678035, whole genome shotgun sequence.
ACCESSION   DAOMPD010000306 DAOMPD010000000
VERSION     DAOMPD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12034_length_3251_cov_4.678035"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLY93_10295"
     CDS             complement(<1..445)
                     /locus_tag="PLY93_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69909.1"
                     /translation="MRFFDMTPRRRTSLSIRKASRLKVKAAVIILNARGYEFSEQSLY
                     RECLALALQFWKGRGGIAQRNTRYNHRKGEYEIVPFYTTEHLRRTVHARGHHAGISIS
                     RMVDFGIEVYLERIIERVLSRMHRGEFMHEVAMWKARYEKRHNRAA"
     gene            complement(628..1869)
                     /locus_tag="PLY93_10300"
     CDS             complement(628..1869)
                     /locus_tag="PLY93_10300"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRP69910.1"
                     /translation="MAEAYIVDAIRTPRARGNKRGSLADVHPQELVATLLKELPKRHP
                     KLDVKKVDDVILGCVTQIGDQAACIARYGVMAAHWPQEVPGYTVNRFCGSGLQAVNDA
                     ATYIQSGTFDLVVAGGVESMSRCKMGSDAGEVTEENMATAMSIGNPKIAAHYNLVPQG
                     ISADIIATKYNISREEADKFAESSQQKADVAIQNGRFKNSVIPVIKDDGSVVDKDEYP
                     NPKWDFKYLSKMPAMFKGVGEKVFDAMALKTYPELESITHIHGGGNASGVVDGSGIVL
                     LASEKAIQQHGLKKRARIVSMAVSGEEPTIMLTGPLSASKKALEKAGLKVEDIDLWEI
                     NEAFAAVPLYVQKQLGIPMDRINVNGGAIALGHPLGGTGAILTGTALDELERRKARYA
                     LITLCIGGGMGIATIIERVDG"
     gene            complement(1953..2174)
                     /locus_tag="PLY93_10305"
     CDS             complement(1953..2174)
                     /locus_tag="PLY93_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRP69911.1"
                     /translation="MSRKAWVIKDDCTSCSLCVDTLPQYFRMDNDDLSETHNKGENVQ
                     DAVVPEADYVKMQEMMDDCPGECIKWKDE"
     gene            2306..>3251
                     /locus_tag="PLY93_10310"
     CDS             2306..>3251
                     /locus_tag="PLY93_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP69912.1"
                     /translation="MAHAWFKSINPQNQRQYSLEDVIYRCPETGALLEVKHDMAHLAQ
                     KSAQDWRALFESRWRSHDKIDASGIWGKREWVLPDLPSEDIISLGEGASTLFSGSQLS
                     QLFAHDELYVKLCGNSHTGSFKDLGMTVLVSQVNHIIKKGAPVRAIACASTGDTSAAL
                     AAYAAYAGILSVILLPKNKISLAQLMQPIANGSLVLALDTDFDGCMQIVQELTQEKEI
                     YLANSMNSLRIEGQKTIGIEVAQQLGWRLPDWFIIPGGNLGNVSALGAGLKMLYELKL
                     VNSLPRIAVAQARAANPLYTSFKQDFKNFAPMTAGETQA"
BASE COUNT          775 a          854 c          760 g          862 t
ORIGIN      
        1 tagccgcccg attgtggcgc ttctcatatc gcgccttcca cattgctact tcatgcataa
       61 actcccctcg atgcatccga ctcaacacac gctcgataat ccgctctaaa tacacctcaa
      121 tcccaaagtc gaccatccgc gagatcgaaa tccccgcatg gtgcccgcgc gcgtgcacgg
      181 tacgccgcaa atgctcggtc gtatagaacg gcacaatctc atactccccc ttacggtggt
      241 tataccgcgt gttccgctgc gcaataccac ccctcccttt ccaaaattgc agcgccaacg
      301 cgaggcattc acggtagagc gattgctccg agaattcata gccgcgtgcg ttgaggatta
      361 ttactgccgc tttcacttta agccggcttg ccttgcggat gctcagcgag gtccggcggc
      421 ggggggtcat atcaaagaat ctcatctata ttataaatac cagaaaatgc tcgatttttc
      481 tcacttttgg aaaaaaaaag tgcaaaaact caaaaaaaaa tgaagttttt tcgtaaaatt
      541 tccggtgggt atagttatcc cttcatgggg taaatagcac tgcggcgggt agtgagaccg
      601 tactcgccgc agcgtataag cgatcgctta accgtcaacg cgttcgatga ttgtggcaat
      661 acccatgccg ccaccgatgc aaagcgtgat gagcgcatag cgtgctttac ggcgttcgag
      721 ctcgtcgagt gcagtccctg tgaggatggc tcctgtgccg cctaaggggt gacccaatgc
      781 aattgcaccg ccgttaacgt tgatgcgatc catagggata cccaattgct tttgtacata
      841 aagcggtacc gcagcaaagg cttcgttaat ttcccataga tcgatgtctt cgactttaag
      901 cccagccttt tcgagtgctt ttttagacgc tgaaaggggg cccgtgagca tgatggtcgg
      961 ttcttcgccc gacactgcca ttgaaacgat acgtgcgcgt ttttttaagc catgctgttg
     1021 gatggctttc tctgatgcta aaagaacaat gcccgaacca tcgacaaccc ccgacgcatt
     1081 gccgccgccg tggatgtggg taatcgattc cagctcgggg taggttttaa gtgccatcgc
     1141 atcgaaaact ttctcgccca cgcccttaaa catcgctggc attttagaaa ggtatttgaa
     1201 atcccatttg ggattgggat attcgtcttt atcgaccacg ctgccgtcgt ctttgataac
     1261 gggaataact gagtttttaa aacgtccgtt ttgaatcgcc acgtcagctt tttgctgcga
     1321 cgactcggcg aatttgtcgg cttcttcgcg gctgatgttg tacttggtag cgataatgtc
     1381 ggcggagata ccctgcggca ctaaattata gtgtgctgca attttgggat taccgatcga
     1441 catcgcggtg gccatatttt cctcggtgac ttcacccgca tccgagccca ttttacagcg
     1501 gctcatcgat tctacgccac cagcgaccac gaggtcaaac gtgccgcttt ggatatacgt
     1561 tgccgcatcg ttgaccgctt ggagtcccga tccacaaaag cggttcacgg tgtagcccgg
     1621 cacttcttgc ggccaatgcg ccgccatcac accatagcgg gcgatacacg ccgcttgatc
     1681 gccgatttgg gtcacgcagc ctaaaataac gtcgtcgact tttttaacat cgagtttggg
     1741 atggcgtttg ggtagctctt ttaataacgt cgccaccaac tcttgcggat ggacatctgc
     1801 caaagaacct cttttatttc cgcgtgcacg cggtgtacga attgcatcga caatatacgc
     1861 ttctgccata ttttacctac cttccttaga gatatattaa cgcattcata gatacgccta
     1921 cgctgcgtaa agagaatcac acgcacaggc tcctactcat ccttccattt aatgcattca
     1981 ccaggacagt cgtccatcat ttcctgcatt ttgacataat cggcttcggg aacaaccgca
     2041 tcttgcacgt tctcaccttt attgtgtgtt tctgacaaat cgtcgttatc catgcggaag
     2101 tattgtggta atgtatcgac acaaagactg caactggtgc aatcgtcttt aataacccat
     2161 gcttttctac tcatattgag atattactaa aactaaacag agcgtcaatt ttttgttgcg
     2221 ttgcattatt tttttgcgac aaaataatga caacgcacaa tactttaagt agttgtccgc
     2281 ctgtttcggc catcaatttt tccctgtggc gcacgcatgg tttaaaagta tcaaccccca
     2341 aaatcagaga cagtattcct tagaggacgt gatttaccgc tgcccggaaa cgggcgcact
     2401 tttggaagta aagcacgaca tggcacactt ggcgcagaag agcgcgcaag attggcgtgc
     2461 tcttttcgag agccgttggc gttcgcacga caaaatagac gcctctggga tttgggggaa
     2521 gcgcgaatgg gttttgcccg atttaccctc tgaagatatt atttctttgg gcgaaggagc
     2581 atcgaccctt ttttcgggga gccaactttc ccaactcttt gcgcacgacg aactctatgt
     2641 caaactctgc ggtaacagtc acaccggttc tttcaaggat ttggggatga cggtgttggt
     2701 atcacaagtc aaccacatta tcaaaaaagg tgcgcctgtg cgtgcgattg cctgcgcttc
     2761 caccggcgac acctcggctg cgcttgcggc gtatgcagcg tatgcgggca ttttgagcgt
     2821 catcctttta cctaaaaata aaatttctct cgcgcagctc atgcagccca tcgcgaatgg
     2881 ctctctcgtt ttagcgctcg ataccgactt cgacggctgc atgcaaattg tgcaagaact
     2941 cacgcaagag aaagaaattt atttagcaaa ctcgatgaac agtctgcgta tcgaagggca
     3001 aaagactatc gggattgaag tggcgcaaca attaggttgg cgtctccccg attggttcat
     3061 catccccggt ggcaacttag gtaatgtctc ggcgttgggt gcggggctca aaatgctcta
     3121 tgaattaaaa ctcgtgaact cgctcccccg catcgccgtt gcgcaagcaa gagctgccaa
     3181 tcctctttac acgtctttta agcaggattt caaaaatttt gcaccgatga ctgccggtga
     3241 aacccaagcg t
//
LOCUS       DAOMPD010000307         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12090_length_3240_cov_2.193093, whole genome shotgun sequence.
ACCESSION   DAOMPD010000307 DAOMPD010000000
VERSION     DAOMPD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12090_length_3240_cov_2.193093"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    219..255
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <256..487
                     /locus_tag="PLY93_10315"
     CDS             <256..487
                     /locus_tag="PLY93_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRP69913.1"
                     /translation="NSSANFLDVEAWEKLADFHAPRLKKGMEILVQGTLKQDRWQDAE
                     GKKHSKFKLIARAIAVSDLKYRPAAEFVKEAA"
     gene            complement(534..1970)
                     /locus_tag="PLY93_10320"
     CDS             complement(534..1970)
                     /locus_tag="PLY93_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRP69914.1"
                     /translation="MIDNYVRKLFSSFLGDQDITLIIGVAYLIASVFFIFGLKFMNSP
                     RTARRGMFMAEIGMGLAIVTTLLDQEIVSLDWILIGLFIGTIAGYIMAIYTPMTAMPQ
                     RTALSHAFGALAAGLVGVTKYYTGHVAGDDHGIMIAVGLEVLLGGLTFTGSMIAFGKL
                     QGVLPGAPIRYKGQNFVNILVIAVLLGSLGYLIYDPSATTLFYVLAFVSLIFGVSLVL
                     PIGAADMPVVMSLLNAYAGLAGCATGFALNNNVLIIAGALDGASGVILSIIMTKAMNR
                     SMTNVLFGGFGAVQATAETHEVKGSIKSQTSEEAAAILDISDSIVIVPGYGMAVSQAQ
                     HSCKKLVDYLIKKGKKVRFAIHPVAGRMPGHMNVLLAEAGVDYDLLCDMESINDDFAT
                     TDVSIVIGANDVVNPAAKEDKSSPIYGMPILNVDDSRTVMVLKRGMSPGFSGIENELF
                     YKDNTVMVFGDAKKTLDGFVVSLQELDA"
     gene            complement(1967..2293)
                     /locus_tag="PLY93_10325"
     CDS             complement(1967..2293)
                     /locus_tag="PLY93_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRP69915.1"
                     /translation="MDTLGPLYIFILAAFVGYTVIHKVPPLLHTPLMAATNAISAISV
                     VGAIIVAGMEHNNPETSTLAIIMAVTAVAAAMTNVVGGFLITDRMLKMFKKDKASDAK
                     KTEGAP"
     gene            complement(2320..>3240)
                     /locus_tag="PLY93_10330"
     CDS             complement(2320..>3240)
                     /locus_tag="PLY93_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRP69916.1"
                     /translation="LKVRPPETHEINGYKDGAWLFATLDPLHRADALAAYEKKKLTTF
                     AMEFIPRITIAQSMDVLSSMASIAGYRAVLLASHEFGRFFPMLMTAAGTIPPAKAFVL
                     GAGVAGLQAIATAKRLGAVVEAFDVRAAAAEQVESLGAKFVKMEITENLQDDQGYAKE
                     ASAEFIAKQKELLHKHIAKSDIVITTAQIFGKKAPILIEDYMVNSMKPGSVIIDLASE
                     TGGNCTLTKHGETVEVNGVKILAPANITSTMASAASRMLSKNMENLLIYLFENGALAR
                     DFATDEILKRSIVTRDGATVSEIVKKATGK"
BASE COUNT          866 a          797 c          766 g          774 t
ORIGIN      
        1 aaaatttgag cgttttctgg tatttaatat atagagggca aacctcaaca acaaaaaata
       61 aacggagaaa aacaatgaat caaccacgaa tgaatttcca acaatttatg gggcgtctag
      121 tacgcgatcc ccaattaaag acgaccccca ccggcaaact ggtgatgacg ttcacgctcg
      181 cgtacaacac gtactcgaaa ggcgatgcga ataactcann nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnntaact caagcgcaaa ctttcttgac gtcgaagctt gggaaaaact
      301 ggccgacttc cacgcgccgc gccttaaaaa agggatggag attttagtgc agggaacgct
      361 gaaacaagac cgctggcagg atgccgaggg caaaaaacat tcgaagttta aattgattgc
      421 acgggcgatc gccgtgtccg atttgaaata ccgccctgct gcggaattcg tcaaagaagc
      481 agcgtagacc aatccatgta ttgcatgggg cttatgcccc atgcaataca caattaagcg
      541 tctaactctt gcaggctgac aacaaagccg tcgagagttt ttttcgcatc tccgaaaacc
      601 atcactgtat tgtctttata gaaaagctcg ttttcaatcc ctgaaaagcc aggactcatc
      661 ccgcgcttta agaccatcac cgtacgggaa tcgtcgacat tcaaaatagg catgccataa
      721 atgggactcg atttgtcttc ttttgcagca gggttcacca catcgttggc gccaatcaca
      781 atcgatacgt cggttgtagc aaagtcgtcg ttgatcgatt ccatgtcgca aagaaggtca
      841 taatccacac ccgcttcggc gagcagtacg ttcatgtggc cgggcatacg gcctgcaacg
      901 ggatggatgg cgaagcgcac tttttttcct ttcttaatga gataatccac aagttttttg
      961 cagctatgct gcgcttgtga aaccgccata ccataaccgg ggacgatgac aatagaatca
     1021 gaaatatcga ggatcgccgc cgcttcctct gaagtttggc ttttaatcga acctttcact
     1081 tcgtgcgttt cagcggtggc ttggactgca ccaaacccac caaaaaggac gttggtcatc
     1141 gagcggttca tcgcttttgt cataattatt gaaagaatga cccccgacgc gccgtctaat
     1201 gcaccggcaa taatgagcac gttgttgttc aaggcaaaac cggtggcaca acccgcaaga
     1261 cctgcatacg cattcaaaag cgacatcacc accggcatgt cggcggcgcc aatggggaga
     1321 acaagactca caccaaagat gagcgaaacg aaggctagta cataaaagag ggtagttgcc
     1381 gaaggatcat aaattaagta accgagtgat cccagtagga cagcaatcac cagtatgtta
     1441 acaaagtttt ggcctttgta acggatcggc gcaccgggca gcacgccctg caatttaccg
     1501 aacgcaatca tggaaccagt aaacgtaagg ccgcccaaaa gaacttctaa accgacggca
     1561 atcataatgc cgtggtcgtc ccctgcaacg tgaccggtgt agtatttagt cacacccaca
     1621 aggccagcgg ctaatgcgcc gaacgcgtgc gaaagcgcag tccgttgcgg catcgcggtc
     1681 atcggcgtgt aaatcgccat gatgtaaccg gcaatggtac cgatgaatag accgattaaa
     1741 atccaatcga gactcacaat ttcttgatcg aggagcgtgg tgacaatcgc aagccccata
     1801 ccaatctctg ccatgaacat ccctcggcgc gcagtgcgcg gtgagttcat aaatttaagg
     1861 ccaaatataa agaagacaga tgcaatcaaa tatgcaaccc caatgatgag ggtgatatct
     1921 tgatcgccta agaacgaact aaaaagtttg cggacgtagt tgtctatcat ggagcgcctt
     1981 ccgttttttt cgcatcactc gctttatctt ttttgaacat tttaagcatc cggtcggtga
     2041 tgagaaatcc acccaccacg ttcgtcatcg ccgccgccac cgctgtcacc gccatgataa
     2101 tggcgagcgt cgaagtttcg gggttgttat gctccatacc ggcgacaata atggcgccca
     2161 cgaccgaaat cgctgaaatg gcgtttgttg ccgccatgag tggggtatgc aaaagggggg
     2221 ggactttgtg gatgactgta taacccacaa atgccgccaa aataaaaatg tagaggggac
     2281 ctaacgtatc cattttaact cctatttatc gtaattgtct tatttaccgg ttgctttctt
     2341 tacaatttca gaaacggttg caccgtcacg cgtgacgata ctgcgtttta aaatttcatc
     2401 ggttgcaaag tcacgcgcta aagcgccgtt ttcaaaaagg taaatgagta agttttccat
     2461 gtttttagaa agcatacggc tggctgcgct cgccatcgtc gaggtgatat ttgccggcgc
     2521 taaaattttg acaccgttca cttcgacggt ttcgccgtgt ttggtgaggg tacagttacc
     2581 gccagtttca gaagccaagt caataatgac cgagcctggt ttcatgctgt tgaccatgta
     2641 gtcttcgata agaatcggcg ctttctttcc aaaaatttgc gcagtggtga tgacaatatc
     2701 cgacttggcg atgtgtttat gcagcaattc tttttgcttt gcgataaatt ccgccgacgc
     2761 ttctttggca tagccttggt cgtcttgcaa attttctgtg atttccattt tgacaaattt
     2821 tgcacccaaa ctctcgactt gctcggcggc ggcggcgcgc acgtcgaacg cttccacgac
     2881 cgcgcccaaa cgcttggcgg tggcaatcgc ttgtaaacct gccacacccg cgcccagcac
     2941 gaatgctttt gcggggggga ttgtacctgc cgccgtcatg agcattggaa aaaaacgacc
     3001 aaattcgtgt gacgctaaga gcaccgcacg gtaacccgca atcgacgcca tcgagcttaa
     3061 aacgtccatc gattgcgcaa tggtaatacg agggataaac tccatggcaa acgtagtgag
     3121 ttttttcttt tcatacgccg caagtgcatc ggcgcggtgt agggggtcga gagttgcaaa
     3181 aagccatgca ccgtctttat acccgttgat ttcgtgagtc tctgggggac gtactttgag
//
LOCUS       DAOMPD010000308         3237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12098_length_3237_cov_2.945317, whole genome shotgun sequence.
ACCESSION   DAOMPD010000308 DAOMPD010000000
VERSION     DAOMPD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12098_length_3237_cov_2.945317"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLY93_10335"
     CDS             complement(<1..191)
                     /locus_tag="PLY93_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69917.1"
                     /translation="MFFLHADEKKVRSTKIFSLAIHAPEDAAFTEIEFSDINLNEASD
                     DEKISGARVKTVRTREKVFR"
     gene            complement(273..1796)
                     /locus_tag="PLY93_10340"
     CDS             complement(273..1796)
                     /locus_tag="PLY93_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRP69918.1"
                     /translation="MERFSRLDWVSIFFAVAATLIGVMVLYGGGVTGEDLAERKIYLL
                     ILCVIAMLIFANINYQLLGSYAPIIYALGLLTLFLVLVPFIGKQVKGARSWIRFAGFG
                     LQPAEFMKLVIVITLSKYLVLRESEIGKLKELILPTVLVAIPALLIAAQPDLGGAVMF
                     MSILFGMLFIGGANTSVLFGFAIIGFVAVFTPMFLEYERYIIVDDIYNSLRETQFRLA
                     DAVRILGFEVWEVAENLSYHTRMVQDQTLVTWAKETLSQGDNLRIFWQTASEIEKNNP
                     NFLRDMLRNSTVMISIGFTGIFFYGLGFLGSFMTGSRWMKRVGNIALIVGLAFGLGFM
                     FNKFVNFKPHQVIRIVSFVNPDKFQKGAGYQLRHSLITVGSGKFAGKGLFQGDMTKGE
                     TPFLPEWYNDFIFSVVGEQMGFWGSGLLLFLLFGLVLRAINIAVTSKDDFGAMLASGI
                     ALIFFLHIFINIGISLGLMPVTGIPLSFVSYGGSHMLLCYICLGILINIYRRRFINA"
     gene            complement(1800..>2115)
                     /locus_tag="PLY93_10345"
     CDS             complement(1800..>2115)
                     /locus_tag="PLY93_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP69919.1"
                     /translation="VQTVREGLRAVTTRGTAAAVFRVPGIIPIAGKTGTVQTRSGAPA
                     DKASQHGWFIGYGPFDGDPKEVLVVGVIVERGVGGAVGAAPIARDVFVKYSQKLKAGT
                     ID"
     assembly_gap    2116..2149
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2150..>3237)
                     /locus_tag="PLY93_10350"
     CDS             complement(<2150..>3237)
                     /locus_tag="PLY93_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP69920.1"
                     /translation="KYKAHEEIILIENLKPEQITVIADYYITFSKFIIRSTSRRFYNL
                     GSAASHLTGYVGLPNQREIAAGIRSYQYTGKNGLEAQYDTILRGDDGEIVQIKSATGD
                     VEEQKVFRDFVPGNNLILTIDTDLQTAASKALGDKTGALIAIRPFTGEVLALASKPDF
                     DPNILIALDNPNRTTHIQQIRENRAELNRAISTKYPPASTYKSLVALAGLEEKRLNAS
                     DHYYCNKTFTLKSSYKGAADATWGDWAKHGDNDLIGALAQSCSVYFYEAGYRMGAEPI
                     IKYSRYFHLGEKSQIDLPNEIPGFIPDSKWKEKTFNTRWFDGDTVNLSIGQGFIETTL
                     IGMVGFYSGIATGGSIYKPHLLRQVRFA"
BASE COUNT          900 a          774 c          743 g          786 t
ORIGIN      
        1 cggaacactt tttctcgagt gcgcaccgtt ttcacccgcg cgcctgaaat tttttcatcg
       61 tcgctggctt cgttcaaatt gatgtcggaa aattcaatct cggtgaatgc agcgtcttcg
      121 ggggcatgaa tcgccagcga aaagattttt gtcgaacgaa ctttcttctc gtctgcgtgc
      181 aaaaaaaaca tggaaagtgt aaataagact aagataattc gcataaaaat ttattgggct
      241 tactttatgt acggtcaacc actttagagc ctttacgcat taataaaccg tcgtctgtaa
      301 atattgatga gtatccctaa acatatataa cacaacaaca tatgcgaacc gccgtaactc
      361 acaaaactca gtgggatacc cgtcaccggc atgagtccca acgaaatacc gatgttgata
      421 aaaatatgta aaaagaaaat gagcgcaata ccagaggcaa gcatcgctcc aaaatcgtct
      481 ttcgaggtga cggcgatgtt gatggcgcgc aacaccaatc cgaaaagtag gaagagcaaa
      541 agtcccgaac cccaaaagcc catttgttcg ccgacgacag aaaaaataaa gtcgttgtac
      601 cattcgggca aaaacggtgt ttcgcctttg gtcatgtcgc cttggaacaa tcctttaccg
      661 gcgaacttgc ccgagccaac ggtgatgagc gaatggcgta gttgatagcc tgcgcctttt
      721 tgaaatttat cggggttcac aaacgagacg attcgaatca cttggtgcgg cttgaagttc
      781 acaaacttgt tgaacataaa ccccaggcca aatgccaaac cgacgataag cgcaatgtta
      841 cccacgcgtt tcatccagcg cgagccggtc ataaaactcc ccaaaaagcc caagccgtaa
      901 aagaaaatac cggtgaaacc aatcgaaatc atgacggttg aattgcgcag catatcgcgt
      961 agaaaattgg ggttgttctt ttcaatttcc gatgcggttt gccaaaaaat tctaaggtta
     1021 tcgccttgtg aaagggtttc tttcgcccac gttaccaagg tttgatcctg caccatgcgg
     1081 gtgtggtaag ataaattttc ggcgacttcc caaacttcaa aacctaaaat ccgcactgcg
     1141 tcggctaaac gaaattgtgt ttcgcgcaat gagttataaa tgtcgtcgac gatgatatac
     1201 ctttcatatt cgagaaacat tggagtgaaa actgcgacga aaccaataat tgcaaaacca
     1261 aaaagtaccg acgtgtttgc ccccccgata aaaagcatac caaagagaat ggacataaac
     1321 atcaccgcgc cacccaaatc gggttgcgct gcgatgagta acgctggtat tgccactaaa
     1381 acggtgggca ggataagctc tttgagttta ccaatctccg attcgcgcaa caccaaatac
     1441 ttgctgagag taatcacaat gacgagtttc ataaactcgg caggctgcaa gccaaagcca
     1501 gcgaagcgga tccacgagcg ggcacctttg acttgtttgc cgataaaagg cacgagaact
     1561 aaaaacagcg tgagaagccc gagtgcataa ataattggcg catacgaacc caagagttga
     1621 tagttaatgt ttgcaaaaat cagcattgcg atgacgcaca aaatcaagag atagattttc
     1681 cgttcggcta aatcttcccc cgtgactcca ccgccataaa gcaccataac accgataagc
     1741 gtagcggcga cggcgaaaaa aatggaaacc caatcgagac ggctaaagcg ttccataatt
     1801 caatctattg tccccgcttt taatttttgt gaatacttta caaacacatc gcgtgcaatg
     1861 ggggcggcgc caacagcgcc acccacgccg cgctcgacaa tcactcccac gactaaaact
     1921 tctttgggat cgccgtcgaa aggaccatag ccgataaacc atccgtgctg cgacgctttg
     1981 tcggcagggg cgcccgaacg cgtttgtacc gtacccgttt taccggcaat ggggataatc
     2041 cccggtactc gaaaaaccgc agcagcggtg ccacgcgtgg tcaccgcacg caacccttcg
     2101 cgcaccgttt gcaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgcgaagcgt
     2161 acttgccgca atagatgagg tttgtaaatc gaaccgccgg tggcaatgcc ggaataaaaa
     2221 cccaccatgc cgatgagtgt tgtttcaata aacccttggc cgattgaaag gttcactgtg
     2281 tccccgtcaa accagcgtgt attaaatgtt ttctctttcc acttgctatc gggaataaaa
     2341 ccaggaattt cattcggcaa atcaatttgg gatttctcgc ctaaatgaaa gtagcgcgag
     2401 tatttgatga taggttctgc acccatgcga taccccgctt cgtaaaaata aaccgagcag
     2461 ctttgtgcaa gcgcaccgat taaatcgttg tcgccgtgct ttgcccagtc gccccaagtt
     2521 gcgtcggcgg cgcctttgta actacttttc agcgtgaacg ttttattaca ataatagtgg
     2581 tcgcttgcgt tgaggcgttt ctcctctaaa ccggcgagag caacgagcga tttatacgtt
     2641 gaggcggggg ggtattttgt cgagatagcg cggttgagct cggcgcggtt ttcgcgaatt
     2701 tgttgaatgt gtgttgtgcg gttggggttg tcgagagcga tcagaatatt ggggtcaaaa
     2761 tcgggtttag acgccaacgc gagcacttcg ccggtgaacg ggcggatggc gatgagcgca
     2821 ccggttttgt cgcccaacgc tttcgaggcg gcggtttgca agtcggtgtc gatagttaaa
     2881 attaaattgt taccgggaac aaaatcacga aagacttttt gttcttccac gtcccccgtc
     2941 gccgatttga tttgcacaat ttctccgtcg tcgccccgca aaatggtgtc gtactgcgcc
     3001 tctaagccat ttttgccagt gtattgatag ctacgaatcc ccgctgcaat ttcgcgttgg
     3061 ttgggtaaac cgacatagcc cgttaaatgc gatgcagcag agcctaaatt gtagaatcga
     3121 cgcgaggttg atcgaataat aaattttgaa aaagtaatgt agtaatcagc aatcaccgta
     3181 atttgttccg gtttcaaatt ttcgattaaa ataatttctt cgtgcgcttt atattta
//
LOCUS       DAOMPD010000309         3237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12101_length_3237_cov_2.564425, whole genome shotgun sequence.
ACCESSION   DAOMPD010000309 DAOMPD010000000
VERSION     DAOMPD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12101_length_3237_cov_2.564425"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLY93_10355"
     CDS             <1..281
                     /locus_tag="PLY93_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRP69921.1"
                     /translation="EDIVDTGLTLQCMIDLLKSRGVKSLEVAALLVKPKNVVLQNKLT
                     YPVKYKAFEISDEFVVGSGLDYKGFMRNLPYIAQVTDNTQLKLFDENN"
     gene            287..721
                     /gene="ruvX"
                     /locus_tag="PLY93_10360"
     CDS             287..721
                     /gene="ruvX"
                     /locus_tag="PLY93_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRP69922.1"
                     /translation="MPENTERLLCIDYGKRFIGFAVANYPGITARALTTFDTQKGDIF
                     TYLQKIIEEEKVNRILLGYPASEIEGEIHLLIQKFRETLSQHTALQIDFVDESNSSQE
                     ADLLLAETRIGRKKKRAQDSEAAKLVLLRYLNAESAIHEPNA"
     gene            complement(747..1373)
                     /locus_tag="PLY93_10365"
     CDS             complement(747..1373)
                     /locus_tag="PLY93_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69923.1"
                     /translation="MSEHFSCSACCGIYNLKKKDEERYKWLEENTQQFLALDLSNTES
                     IIRFRREREMVTLPQRVREDTYVCPFVGFVHNQKMGCLLHPVGSPHPQISILPHPQNF
                     SFYGEGICQSYDCLAKERRAHRADFFRWAETAPFFAYSRLASDHTLHRALAGMDLGEC
                     DLAPFYDLLSQLYEEYNVVVTSFENIEKEVPSSWEDLWLYLANNIVCM"
     gene            complement(1435..2694)
                     /locus_tag="PLY93_10370"
     CDS             complement(1435..2694)
                     /locus_tag="PLY93_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP69924.1"
                     /translation="MNMTTTTAKQTFSSYEKFVIAILTFLQFTVILDFMILSPLGTFL
                     IPKLKITPEQFGWVVSAYALSAGISGITAAGFADRFDRKKMLLFFYTGFTLGTLFCGL
                     APDYHTLLASRIFTGIFGGVMNSIAFAIITDLFPMQVRGRVMGFVQMAFASSQILGIP
                     MGLFFANHFGWHFSFFMIVAVAFPVGIVIFFKMRPIDKHLKIKSERNPIAHLVATATK
                     PRYLRGFVATMLLATGGFMLMPFGTVFLTGNLKIDAADLPIIYTVTGMAALLTGPFAG
                     RMADRFGKYQVFTTASLLGIGVVLYYTQLQVATVAFITAISAILFVFITARIVGATTL
                     ISGVPATQDRGAYMGVSSAVQQISGGIASAVAGLIVFQRADGSLGNYRILGYVVAVTM
                     ALTILLMYIIHRMVVISVTPQPLLSHE"
     gene            complement(2781..>3237)
                     /locus_tag="PLY93_10375"
     CDS             complement(2781..>3237)
                     /locus_tag="PLY93_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRP69925.1"
                     /translation="TDIGAINSKMQLGRICALADSGVAEGAVMHQKTGKLPDKGFWFQ
                     PTFFTNVTPSSRIAREEIFGPVLSVMTFRTPEEAIEKANNTPYGLSAGIWTDKGSKAM
                     KMANAVKAGVVWINTFNKFDPTSPFGGYKESGYGREGGKQGLLDYCKQE"
BASE COUNT          986 a          755 c          709 g          787 t
ORIGIN      
        1 ccgaggacat tgtcgacacc ggccttactc tccaatgcat gatcgacctc ttaaaaagtc
       61 gaggcgtgaa atcgctcgaa gtggctgccc ttttagtaaa acccaagaac gtcgtgttgc
      121 aaaataaact tacctacccc gtcaaataca aagcttttga aatttccgat gagtttgtcg
      181 tcggctctgg tctcgactat aaaggtttta tgcgcaactt gccctacatc gcgcaggtca
      241 ccgacaacac gcagcttaaa ctctttgacg agaacaatta aagaccttgc ccgagaatac
      301 cgaacgactt ctctgcatcg actacggcaa acgcttcatt ggttttgccg tcgccaatta
      361 cccggggatt accgcacgcg cactcaccac gtttgacact caaaaaggag atatctttac
      421 ctatctacaa aaaattatag aagaagaaaa agtaaatcgt attttattgg gttaccctgc
      481 atctgaaatt gaaggagaaa tccacctctt gattcaaaaa tttcgtgaaa ctttgtcgca
      541 gcacacagcg ctacagattg actttgtgga cgaatcgaat agcagccaag aggctgacct
      601 acttttggcg gaaacgcgca taggtcgcaa aaaaaagcgg gcacaagaca gcgaggcggc
      661 caagctggtg ctccttcggt atttaaatgc tgagtcggca atccatgagc caaacgctta
      721 aaggattata ttatattcca agattgtcac atacacacga tattattagc aaggtatagc
      781 cataaatctt cccagctgga tgggacttct ttttcaatgt tttcaaacga cgttaccact
      841 acattatatt cttcgtagag ttgcgagagt aaatcataaa acggcgccaa gtcgcactcg
      901 cctaaatcca tacccgctag cgcgcggtgg agcgtatggt cggaagctaa acggctatat
      961 gcaaaaaacg gcgccgtttc agcccaacga aaaaaatccg cacggtgggc gcgtctttct
     1021 ttcgcaaggc aatcatagct ttggcaaatc ccttcgccgt agaaggaaaa attttgtgga
     1081 tgcgggagga tggatatttg tggatgcgga gaaccaacag ggtgtaaaag gcaacccatt
     1141 ttttgattat gtacaaaacc aacaaacgga cacacatacg tatcctcacg cacccgttgc
     1201 ggtagtgtca ccatttcgcg ttcacgccga aaacgtataa tactttcggt attcgataaa
     1261 tcaagagcta aaaattgctg cgtattctct tcaagccatt tatacctttc ttcgtccttt
     1321 tttttaagat tataaatacc acagcaagcg ctacatgaaa aatgctcact catgcaagga
     1381 ttgatggctc gggtgcttat atcatgaagt gtcttttctt ttggaagcgg agcgctattc
     1441 atgcgacaat aagggttgcg gtgtaacgga aataacaacc atacgatgta ttatgtacat
     1501 aagtaaaatt gtgagcgcca ttgtcactgc gaccacataa ccgagaattc tataattacc
     1561 cagagaacca tcggcacgct gaaaaacgat aaggcccgct acagccgacg caataccgcc
     1621 ggatatttgt tgtacggcag aactaacgcc catataggcg ccccggtctt gcgttgctgg
     1681 cacgccgcta ataagtgtcg ttgcacccac tatgcgtgca gtaataaaca caaacaaaat
     1741 tgcagatata gccgtgatga atgccaccgt cgctacctgc aattgagtat aatagagaac
     1801 gacccctatt cctaaaagcg aagcggtggt aaaaacttgg tacttaccaa acctatcagc
     1861 catacggcca gcaaaagggc cggtaaggag agcagccatg ccggtgactg tgtagataat
     1921 aggtaaatct gccgcatcga tttttaaatt acccgttaaa aaaacagtcc caaagggcat
     1981 gagcataaac cctcccgttg caagaagcat cgttgcgaca aacccgcgta aataacgtgg
     2041 tttagtggca gtggcgacaa ggtgtgcaat cgggtttcgc tctgatttga ttttaagatg
     2101 tttgtcgata gggcgcattt taaagaatat aacaatacca actggaaaag caacggcaac
     2161 aatcataaaa aacgaaaaat gccagccaaa atgatttgca aagaaaagcc ccattgggat
     2221 tcccaggatt tggctcgacg cgaacgccat ttgcacaaac cccataacgc gtccgcgcac
     2281 ttgcatcgga aaaagatcgg tgataatggc aaatgcaatg gagttcataa cgccgccaaa
     2341 gataccggta aaaatacgtg acgctaaaag cgtatggtaa tcgggagcaa gcccgcaaaa
     2401 aagtgtacct aaagtaaaac cggtataaaa aaagagtaac atttttttgc gatcaaatcg
     2461 atctgcaaac cctgcagcgg tgattcccga aatacccgcg ctcaaagcat acgcagaaac
     2521 aacccaaccg aattgttcag gcgtgatctt aagttttggt attaaaaatg tcccgagagg
     2581 agataaaatc ataaaatcaa gaataacagt aaactgcaaa aaagttaaaa tcgcaatgac
     2641 aaatttctcg tagcttgaaa aggtttgttt tgcagtagta gtagtcatat tcatccaggc
     2701 aatttttgaa aggtcgttaa cgcgacgaga gaaatgacta cacaaactat gatttggtca
     2761 ggagcctgtg cggtaatcca ctactcttgc ttacaataat ccagcaaacc ttgcttgcca
     2821 ccctcgcggc cataaccgga ttccttgtag ccaccgaaag gcgaagtagg atcaaatttg
     2881 ttaaaagtat tgatccacac gacacccgct ttaactgcgt tggccatttt cattgctttt
     2941 gaacctttgt ccgtccagat acctgcagaa agtccgtacg gggtattgtt tgctttttcg
     3001 atggcctctt cgggggtgcg gaatgtcatc accgacaaga ctggtccaaa aatttcctcg
     3061 cgtgcgatgc ggctcgacgg tgtgacgttc gtaaaaaatg tcggttggaa ccaaaaacct
     3121 ttatcgggca gcttaccggt tttctggtgc atcactgccc cctcggcgac acccgagtcg
     3181 gcgagcgcac aaattctgcc gagctgcatt ttagaattga tggcgccaat gtcagtg
//
LOCUS       DAOMPD010000310         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12113_length_3235_cov_2.541509, whole genome shotgun sequence.
ACCESSION   DAOMPD010000310 DAOMPD010000000
VERSION     DAOMPD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_3235_cov_2.541509"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2245
                     /locus_tag="PLY93_10380"
     CDS             <1..2245
                     /locus_tag="PLY93_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69926.1"
                     /translation="YQLTVSGVQDINGNTIAAPGTLQFQCGNDTTPPSLLSVTVVSAT
                     AGSTVLMLNFSEAVDNVTGNVMANYTGDYKNNTGGDLAGILSNWGGSTANASNLITAG
                     RQGNTAQVKLTFQPALPNGGHQIGVKNVTDLVSNVILSNSVNNVQPVIVNAPTGFVGG
                     PVFDDPFGDGTAAGLITVYDGKIYLGTDKNSSKLFEMNYGLTQAQTITLDADGTFGAP
                     YENFEGYVSRFSGCTGSFSNPPISADGCNANQVQGVDAIYSACVGGTSIPQLTGTACT
                     SISGAHEYMFIASRRTNSIGSARYRSFWYTETKSSSSTVFPFTEGFNGDTNGGTYSYR
                     AMNVIVFKDYLFVNMGTEQGGGGRGGRVCVNPAGCAGGYAYLSYVQLPNTSSLTRIGA
                     QSNTSGGNRKNGSFNSNDGGNYRGPGEAGDTDTGILNAITAMYEYDNDGTGGNESQLY
                     LANGGFYNGALTAARVTTTDGGIVRTKLTYSSKSSLPLDCATGGNGCTNYYEDVTPDA
                     KPEWYKYLSIPLPQNSSAVSASNCATGYVEMECTLPYNLFVPALKAIPYMRTAPNGDL
                     YLARNACDTNNLNRNGKNGAGGADFRTERQVCPKGHEVPQLWMLPKGTTASPKGAADW
                     VLVAQYGTSGKTNMNGQNAIGAHNSHISLLEFVGSHLYVGWDNGTDGANVWRTDMTSV
                     ASGSTPAESSFSMVNLPGIESGGGGSNQKIFSHVTVNDGGKDWLILTTRDGSNAMKIY
                     RTANGQN"
     gene            complement(2296..>3235)
                     /locus_tag="PLY93_10385"
     CDS             complement(2296..>3235)
                     /locus_tag="PLY93_10385"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRP69927.1"
                     /translation="CARIRLGKELMNYFDAVFWQVAVIAPKKQALLVEDFFLNAGALS
                     TYELLYAEGKTHNLTEENTTLYFFFEEKFPIHAFTPMALATIGLAELPFTIAQVEYAD
                     YLKEFEKSFKAFELTQKTALVPPWDENNAVLPPHLKSLYLVPGMAFGTGKHSTTQLMV
                     EFIEETVRPTDTVLDMGTGSGILAIAALLYGAQKAYGFDVETLAVESAAANLELNVQK
                     YNRPFNAHFQKGDFTSLDSILCDKAITVFLANILPNIFEANATSLKAALYNCRAWALS
                     GIPTAQGDAFSAFLKTITPHPFLTREKDEWLVVYHR"
BASE COUNT          830 a          735 c          860 g          810 t
ORIGIN      
        1 gtatcaactg acggttagcg gtgtgcaaga tataaacggc aacaccattg ctgcacccgg
       61 tacattgcag tttcaatgcg gtaacgatac cacaccgccc agcctcttga gtgtcaccgt
      121 ggtttctgct acggcaggtt cgactgtgct gatgctcaat tttagcgagg ctgtcgataa
      181 cgtcaccggt aacgttatgg ctaattacac gggagattat aaaaacaaca ctggtggcga
      241 ccttgcgggt atattgtcca attggggcgg tagtaccgcc aatgcttcga atttgattac
      301 tgcaggcaga caaggtaata cagcgcaagt gaagttgacg tttcagccgg cgctccccaa
      361 cggtggccat caaatcggcg taaagaatgt aaccgattta gtgagtaatg ttattttaag
      421 taacagcgtg aacaacgtgc aacccgtcat cgtcaacgcc cccactggct ttgtcggtgg
      481 tcctgtgttc gacgacccct ttggcgacgg cacagcggca ggactcatca ctgtgtacga
      541 tgggaaaatt tatttgggta cagataaaaa ctcgagcaag ctttttgaaa tgaattatgg
      601 cttaacacaa gcgcagacga ttactctgga tgccgatggc acgtttggtg caccgtatga
      661 aaattttgaa gggtatgtat cgcgtttctc gggctgtaca ggttcatttt caaatccacc
      721 tatttcagca gatggttgta atgcaaacca agtgcaggga gtggacgcca tttattctgc
      781 ttgcgtcgga gggacttcaa ttccacagct tacaggcacg gcttgtacaa gcatttcggg
      841 tgctcatgaa tacatgttca tcgcttcacg cagaaccaac agtatcggta gcgcgcgcta
      901 tcgatcgttc tggtacacag agaccaagtc aagttcgagc actgttttcc ctttcacaga
      961 agggttcaac ggtgacacca atggtggaac atactcatac cgtgctatga atgttatcgt
     1021 gttcaaagac tacttgtttg tgaatatggg aacagaacaa ggcggcggcg gtcgcggtgg
     1081 tcgagtgtgc gtcaatccgg ctggctgcgc gggaggctat gcatacttaa gctatgtgca
     1141 attaccgaac acgagcagct tgacccgcat tggagcccaa tccaatactt cgggaggcaa
     1201 tcgtaagaat ggttcgttta attcaaacga tggtggtaac tatcgcgggc ctggcgaagc
     1261 gggtgataca gacaccggaa ttctgaatgc aattacggct atgtatgagt acgacaatga
     1321 tggtacgggc ggtaatgaat cgcagctcta cctcgccaac gggggattct ataatggtgc
     1381 acttaccgct gctcgggtaa caaccacaga tggtggtatt gttcgcacga aactcacgta
     1441 ttcgtctaaa tcctcgctac ctttggattg cgcaacgggt ggaaacggat gtactaacta
     1501 ttacgaagac gtcacaccgg atgcaaaacc agagtggtac aagtatcttt cgatacccct
     1561 accgcaaaat tcgtccgccg taagcgcttc caactgtgcg acaggctacg ttgaaatgga
     1621 atgcacgtta ccgtacaatc tctttgtacc tgcgctcaag gcgataccgt acatgcgtac
     1681 ggctccgaat ggggatttat atctggcgcg caatgcgtgc gacacgaata acttgaatcg
     1741 aaacggaaaa aatggtgctg gcggtgccga ttttcgtaca gaaagacaag tttgtcccaa
     1801 agggcatgaa gtgccacaac tatggatgct tcccaaagga actacggctt cccccaaagg
     1861 agcggcagat tgggttctgg tcgctcaata tggcacttca ggaaaaacca acatgaacgg
     1921 tcaaaatgca atcggtgcgc ataactcgca catttcactg cttgaatttg tagggagcca
     1981 tctctatgtt ggttgggata acggcaccga tggtgcgaac gtgtggcgta ctgatatgac
     2041 gagcgtcgcg tcgggttcaa cccccgccga gtcgagtttt tcgatggtaa acttaccggg
     2101 cattgagtcg ggtggtggtg gttccaacca gaaaattttt agccatgtca cggtgaacga
     2161 tggcggtaaa gactggttga ttctgacgac ccgcgacggt tcgaacgcga tgaagattta
     2221 tagaaccgcg aacggtcaga actaaggaag ccgtgcgggt agaatgacta aattttatag
     2281 ggtggtgtgt tgctcttaac ggtgatacac caccagccac tcgtcttttt cgcgcgtgag
     2341 aaagggatgc ggggtgatgg tttttaagaa tgcgctaaac gcatcgcctt gtgcagtggg
     2401 gattcccgaa agcgcccatg cgcggcagtt gtaaagagca gctttgagag aggttgcgtt
     2461 ggcttcaaaa atattgggta aaatgttcgc caaaaatacg gtgattgctt tatcgcaaag
     2521 gatagagtcg agacttgtga aatccccctt ttgaaaatgt gcattgaaag ggcggttgta
     2581 tttttgtacg ttgagttcta aattggccgc cgccgattcc actgctaacg tttcaacgtc
     2641 aaatccgtat gccttttggg cgccataaag caacgcagca attgctaaaa tccccgaccc
     2701 cgtccccatg tcgagtaccg tgtcggtggg gcgtaccgtc tcttcgatga actccaccat
     2761 gagctgagtg gtggagtgtt tgccggtgcc aaacgccata cctggcacaa gatacaacga
     2821 ttttaagtgt ggtggaagca cggcgttgtt ttcatcccat ggcggaacga gggcagtttt
     2881 ttgtgtgagt tcaaacgctt tgaaactttt ttcaaactct ttgaggtaat cggcgtactc
     2941 cacttgcgcg atggtaaatg gaagctcggc aagcccgatt gtcgctaagg ccatgggagt
     3001 gaaagcgtgt atcggaaatt tctcttcaaa gaaaaaatag agagtggtgt tttcctcggt
     3061 taagttgtgc gtcttgcctt cggcgtacaa aagctcgtac gtgctcaacg caccggcgtt
     3121 caaaaaaaaa tcttcgacaa gaagcgcctg ttttttcggt gcgataacgg cgacttgcca
     3181 aaaaaccgcg tcgaaataat tcatgagttc tttgccaagg cgaattcgtg cgcaa
//
LOCUS       DAOMPD010000311         3209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12237_length_3209_cov_3.415346, whole genome shotgun sequence.
ACCESSION   DAOMPD010000311 DAOMPD010000000
VERSION     DAOMPD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12237_length_3209_cov_3.415346"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..648)
                     /locus_tag="PLY93_10390"
     CDS             complement(19..648)
                     /locus_tag="PLY93_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69928.1"
                     /translation="MSKLGRHYAENEMRHYLDEKLDAKAAEGLKQHLETCRECFTLYM
                     EIREIEYLQHIGQGASAALRERVKTFARQASGKLIRVRFKVLRDKFILMSGDSENLEF
                     QGVRANFAYRGKTERGPVSFSHKIDNQLVTLTLIPVEGKKNYKVAVDFSGKKEKICAH
                     LNLHGKTVETIADLRKQAQFLSIVERGQEIDLRFEKSGKELFTINFGID"
     gene            complement(666..>1200)
                     /locus_tag="PLY93_10395"
     CDS             complement(666..>1200)
                     /locus_tag="PLY93_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP69929.1"
                     /translation="RSPIQQMLLACTEGDDAAWDTFITLVQGVISGIGRKYVVNDVED
                     FCQNVYVLLMSNDFALMRRFKGDAEFELLRYIRSIAWREAKNQSRKNRVKERRIQNLG
                     GEPVDATQNSDTADLRIVMNSTLMKLPFEEREILTLVAVGYKFREIADILKKPLNTVI
                     SKANRTQKMLKKRPELQ"
     assembly_gap    1201..1210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1352..1627
                     /locus_tag="PLY93_10400"
     CDS             1352..1627
                     /locus_tag="PLY93_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRP69930.1"
                     /translation="MRIQEDIKPISYFKAHSAEVLEQINKTHRPFYVTQNGEAKAVVL
                     APETFEEMNNAIKILKIVSMSEQDIREGRVLTQTNVENQLKKKFCIK"
     gene            1635..1967
                     /locus_tag="PLY93_10405"
     CDS             1635..1967
                     /locus_tag="PLY93_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRP69931.1"
                     /translation="MPKNEIIWAKYAYADLDGILDRINRNNPENVPKIYQKIMSKIKS
                     LETFSNIGRVIPELERLNIFIFREIFTNPWRIFYRAEKQRVYILAIFDGRRDLEEIII
                     KRLLFEKS"
     gene            complement(2098..2745)
                     /locus_tag="PLY93_10410"
     CDS             complement(2098..2745)
                     /locus_tag="PLY93_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69932.1"
                     /translation="MKKKVVKMKNGLLGRIVDWSRNIGALPMKLVFRISLVISLFAIV
                     FFVSDIEAKSKKGKKKGSNIFPAQVVKNYFTASSQASFSPVKSKLKRAEAQMRQQITI
                     GKKYTLAKHCIGTLDFSVSEQQYFSNILCTKNGEPSKKYDDYEYMAAQLIFESVPESA
                     EELFGQDFRGIGGSTDIKYARFTFVLTTGFDWMTIQTATAKANLIQATPFKLDND"
     gene            complement(2791..3069)
                     /locus_tag="PLY93_10415"
     CDS             complement(2791..3069)
                     /locus_tag="PLY93_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69933.1"
                     /translation="MKRWDINHANTISLLVTGLEYPRLMVLRGEIFETVRGVKAINLK
                     GSFGRAATLEVEFEGSSSDLADRLLSSGLSIRLKVGEVKPNALDIQAK"
BASE COUNT          868 a          732 c          620 g          979 t
ORIGIN      
        1 ttgtacattt ctatctcctt aatcaatccc aaaattaatt gtaaacaact ctttgccgct
       61 cttctcaaaa cgtaagtcaa tctcttgccc ccgctcgaca atcgacaaaa attgcgcctg
      121 cttgcgcagg tcggcgatgg tttctaccgt tttgccgtgc aagtttaagt gggcacaaat
      181 tttttctttc ttaccggaaa aatcaacggc cactttgtaa tttttcttac cctcaaccgg
      241 tataagcgtc agcgttacca gttggttgtc tattttgtga gaaaaactga cagggccgcg
      301 ctcggttttg ccccgatacg caaaattggc gcgcacaccc tgaaactcca aattttcgct
      361 gtctccgctc ataagtataa acttgtcacg caaaacctta aaccgcacac ggattaactt
      421 gccgctcgcc tgccgcgcaa aagttttaac tctctcgcgc aacgccgccg acgcgccttg
      481 gccaatgtgc tgtaagtatt cgatttcgcg tatctccata taaagagtaa agcattctct
      541 gcatgtttca agatgctgct ttaacccctc tgccgctttt gcatccagct tttcgtcgag
      601 gtagtgccgc atctcgtttt ctgcgtaatg tcttcctaac ttactcatgt gcataccccc
      661 gatttttatt gcaactccgg tcgttttttt aacatttttt gtgtgcggtt agcctttgag
      721 ataaccgtat tcaagggttt tttcaaaata tctgcaattt cacgaaattt atagcctacg
      781 gctaccaatg tgaggatttc cctctcttca aaagggagtt tcatcagcgt gctattcatc
      841 acaatgcgta aatcggcggt gtcactattt tgggtggcat ccaccggctc gccgcctaaa
      901 ttttgtatac gccgctcttt tactctattt ttacggcttt gatttttagc ctcgcgccac
      961 gcaatcgaac ggatatagcg taatagttca aactctgcat cgcccttaaa acggcgcatg
     1021 agcgcaaagt cgtttgacat caaaagcaca tacacgtttt gacagaaatc ttcaacatcg
     1081 ttgacgacat acttgcgccc aatacccgaa ataacgcctt gcaccaaggt gataaacgta
     1141 tcccacgcgg catcgtcgcc ttcggtacac gcaagcagca tttgttgaat cggcgatctt
     1201 nnnnnnnnnn catagcggcg caaattctac aagtttgtag cgtccgctaa ctgcgtcaat
     1261 gagtttgtgg agtttcatgc gcccgtaagc tttagccgat gaagtttaaa tttcttgacg
     1321 ctaagactga atatagtact tttttcggtc tatgaggatt caagaagaca ttaagccaat
     1381 atcttatttt aaggcgcatt ctgcagaggt tcttgaacaa ataaacaaaa ctcaccggcc
     1441 attttatgta acacaaaacg gagaagcaaa agccgtagtt ttagcccctg agacatttga
     1501 agaaatgaat aatgctataa aaattcttaa aatagtatcc atgagtgaac aagatattcg
     1561 agagggtcga gttttgactc aaacaaatgt agaaaatcaa ctgaaaaaga aattctgcat
     1621 taaatagcag attgatgcca aaaaatgaga taatctgggc aaaatatgcc tatgccgatc
     1681 tagatggtat tcttgatcgc attaatcgta ataatccaga gaacgtacct aagatctatc
     1741 aaaagattat gtctaaaatt aagtcgcttg aaactttttc taacattggg cgcgtaatac
     1801 ctgaactaga aaggctaaat atctttatat ttcgtgagat ttttacaaat ccatggcgca
     1861 tattttacag agcagaaaag caaagggtct acattcttgc catttttgat ggccgccgtg
     1921 atcttgagga gattattatt aaaagattgc tttttgaaaa gagttaagtg aggcatagcg
     1981 gcgcgaattc tacgagtttg tagtatttca tgcaccgcgg gcgcggcgca tgaaatacta
     2041 tactaagcct accgcgctta agtcatccgc accgagccgg gagatgactt tatttgttta
     2101 atcgttatcg agtttaaacg gtgttgcttg gattaagttt gccttagcgg ttgctgtttg
     2161 tatagtcatc cagtcgaaac cagttgttaa tacaaaagta aaacgtgcat attttatatc
     2221 ggtactgccg ccgatgccgc gaaaatcttg accaaagagt tcttctgctg attcagggac
     2281 gctctcaaaa attaattgtg ctgccatgta ctcgtagtca tcatatttct tacttggttc
     2341 accatttttt gtgcaaagaa tatttgagaa atactgttgt tctgaaacgc taaaatcaag
     2401 tgtgccaatg caatgctttg ccagcgtata tttcttccca atagtgatct gttgtcgcat
     2461 ttgagcttct gcgcgcttga gtttagattt gactggtgaa aaagaggctt gagagcttgc
     2521 ggtgaaataa ttttttacta cttgtgctgg aaaaatattc gagcctttct ttttgccctt
     2581 ttttgattta gcttctatat ctgatacaaa gaaaactata gcgaaaagac ttatcacaag
     2641 cgatattcga aagacaagtt tcataggcag tgctccaata ttgcgagacc aatctactat
     2701 ccgccctagt agcccgtttt tcattttcac aactttcttt ttcataattt acctctttca
     2761 aatttatttt ttgcatatat tattatgcca ctatttcgcc tgtatgtcga gcgcgttggg
     2821 tttcacttct cccactttta ggcgaatgct taaccccgaa ctcagtaaac ggtcggctaa
     2881 gtcgcttgag ctgccctcaa attcgacttc gagcgtcgct gctcggccaa aagagccttt
     2941 gaggttgatc gctttcacgc cgcgcaccgt ctcaaatatt tctccacgca acaccatcaa
     3001 acgtgggtac tcgagaccgg taaccaacaa gctaatcgtg ttggcgtggt taatgtccca
     3061 gcgtttcata atctgcgcct tcgcacaagc catactgtta ccgagtctgt gcataaatgc
     3121 cataaatttc taatttgtac catacctttc ccatggagcc tgtgcgccaa ctcgcacacc
     3181 aagcccttct tcccttctaa aaatcaaaa
//
LOCUS       DAOMPD010000312         3194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12308_length_3194_cov_2.192737, whole genome shotgun sequence.
ACCESSION   DAOMPD010000312 DAOMPD010000000
VERSION     DAOMPD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12308_length_3194_cov_2.192737"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLY93_10420"
     CDS             <1..851
                     /locus_tag="PLY93_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP69934.1"
                     /translation="AYGGTLELLNVQLRSLNIPIHFLLGNEISQLDKLLSDGAKMVFY
                     ETPTNPTLEIFDIALISEKAKKHGALTVIDNTFASPINQNPLRLGADIVVHSATKYLG
                     GHSDITAGLVMGKREHINEIMPWRKNLGQTPAPETASMLARSIRSLTPRVKWQNESAM
                     FIASKLEKHKAIKRVYYPGLESFPGHQTAKKQMHGFGGMLTIELNADLERTVRVVDAF
                     KIFKIAPSLGGVESLATQPVTTTHHGLSEQERKRRGISDSMIRLSIGLEDPADLLEDL
                     QQALDY"
     gene            complement(1272..>1546)
                     /locus_tag="PLY93_10425"
     CDS             complement(1272..>1546)
                     /locus_tag="PLY93_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRP69935.1"
                     /translation="YCTNKLRYKSMVTVSMNNVYFRAPGYLGDIIQIYARLKEVRRSS
                     VTAEGKAVAYDPEKQTSREIITCEVTYVAVSPTGKPVRIFDEHGKA"
     assembly_gap    1547..1587
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1707..>3194)
                     /locus_tag="PLY93_10430"
     CDS             complement(1707..>3194)
                     /locus_tag="PLY93_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP69936.1"
                     /translation="QKLQGLPQVGSSDENEVLWRKAQNLGKPFNSTFDDEYPTVVRNG
                     NYMYFASNRENGQGSFDVYRAKVPDFAKPEVVVTYKGRVKEKFSDKGIEANIRITDVD
                     GERNFSTQETDGLYSADLVNKKQYKMVVTAPGYTPVEYIADLRNINSPVVIQKDFELE
                     RIVTLPKEATLYLTFVDEKGKVLKPQATYTLAPENPNEAVVRFQKNKGVLQVPLMAKY
                     KNIDDAMAALQKMQLGVSAQKKGYADLHETRSLADALRGANGETLDKVELKFVMKEGE
                     APAVHITECKAGNGCVAIAYFSTNIADKLNNEKAAGLKAVVELWNKEPHKYVYVYGHT
                     DSRGGASYNMKLSKARADFVKKRLVEYGIPAEMVITKAMGETQLANKNEKTDAGRRLN
                     RRAEIYFDTQKRSDNAVIQEKAAPKTVKPKKNVKKKATKKKVDKKRKPLAPEPVEPSA
                     KKPQAEDNPTPSTPAVKEESAPEGTEETQKTEEKSTSPHTEIKIQ"
BASE COUNT          755 a          709 c          744 g          945 t
ORIGIN      
        1 atgcatacgg tggtacgctt gagctactaa acgtccaact aaggagtctt aatattccaa
       61 tacattttct tttagggaat gaaattagtc aattggataa attattgagt gacggtgcaa
      121 aaatggtgtt ttatgaaaca ccaaccaacc cgactttaga aattttcgat attgcactca
      181 tctccgaaaa agcaaagaag catggagcgc ttaccgtaat cgataacacc tttgcctctc
      241 cgataaacca aaacccatta cgattaggcg ctgatattgt cgtacatagc gcaacaaaat
      301 atctcggtgg tcacagcgat attacggcag gcttagttat gggtaaacgt gaacatatca
      361 atgaaataat gccatggcga aagaatttag ggcagacccc ggcaccggag acagcgtcga
      421 tgctggctcg tagtatccgc tctctgacac caagggtgaa gtggcagaat gaatccgcta
      481 tgtttatcgc ctcaaaatta gaaaaacata aagcaataaa acgagtatat taccctgggt
      541 tagaatcttt ccctggacat caaactgcca aaaagcaaat gcacggtttt ggtggaatgt
      601 tgacgataga actgaatgca gatttagaaa gaactgtcag ggttgtggac gctttcaaga
      661 ttttcaaaat cgcgccgagt cttggcggag tagaaagtct tgctacccaa ccagtgacta
      721 caacccacca cggactcagc gagcaagaac gtaagcgcag gggaatctcc gactcaatga
      781 tacgcctttc cattggcttg gaggatccgg cagatctgct ggaagatttg cagcaggctt
      841 tagattattg aaaatactat tctacctatt cctgcacgat ggaattgttt ctcgcggcaa
      901 aatgaaagaa taggcgccaa ccgattacgc ccacgttcgt tttaaggtat atggcggcac
      961 gtgtattcta tatactctgc gtaaaaagcc tgtgcgccat gaacattaac cacatacgca
     1021 caggctcgaa ggcacgtaaa gtctctaaaa aatcttgttg atttttccgg attatgtcat
     1081 attagcggat aactatgccg gttttctgat tatgtcacat taaggggtaa ctatgcactg
     1141 cgggtactgg gctgctcaaa atcgaggaaa ttttaaagaa aaactttctt tatatacatg
     1201 tagattattg attcttcaac ccatttaggc atagctggca gtgtctggtt ggatgcttac
     1261 cgcacagact cttaagcctt cccgtgctca tcaaagatac gcaccggttt tcccgtaggc
     1321 gatacggcga cgtaggtgac ttcgcaggtg ataatttcac gcgaggtttg tttttcagga
     1381 tcgtacgcca cggctttacc ctcggcggtt acgctcgaac ggcgcacttc ttttaagcgt
     1441 gcatatattt gaatgatgtc gcctaaatag ccgggcgcgc ggaaatagac gttgttcata
     1501 ctcactgtca ccattgattt gtagcgcaat ttattagtgc aatatannnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnacg ccaaaagttg cccgccaaaa atatggtggt
     1621 tggggttcaa gtgctccgac atcaccatgt gctgcgtgac caactcaaac ggttcgttgt
     1681 ttgtatttcc ggtaggatga cgcatgttac tgaattttaa tttctgtatg cggtgatgtg
     1741 ctcttctctt cggtcttttg cgtttcctca gttccctcgg gtgcactttc ttctttcact
     1801 gcgggtgtgg agggtgtggg gttgtcctcg gcttgtggtt ttttcgccga gggttcaacc
     1861 ggttcgggag cgagcggctt gcgcttttta tctactttct tttttgtcgc tttcttcttt
     1921 acattcttct tcggctttac cgttttaggc gccgcctttt cttgaatcac tgcattgtcc
     1981 gagcgttttt gcgtatcgaa ataaatttca gcgcggcggt tgagacggcg tccggcgtca
     2041 gttttttcgt tcttgtttgc cagttgggtt tcacccatcg cttttgtaat gaccatttcg
     2101 gcagggatac catactcgac caagcgcttt ttcacaaagt cggcgcgcgc tttggaaagc
     2161 ttcatgttgt acgatgcacc gcctcggctg tcggtgtggc cgtacacgta cacgtatttg
     2221 tgaggctctt tattccagag ttcaacgacc gccttaagac cggcggcttt ttcgttgttg
     2281 agtttatcgg cgatattcgt tgaaaaataa gcgattgcca cgcagccatt gcccgctttg
     2341 cattcggtga tatgcaccgc cggcgcttcg ccttctttca tgacgaattt tagttcaact
     2401 ttatctaaag tttcaccgtt tgctccacgt agtgcatcgg ccaacgaacg ggtttcgtgc
     2461 aagtcggcgt atcctttttt ctgcgcagaa acgccaagct gcattttttg gagcgcggcc
     2521 atcgcgtcgt cgatgttttt atattttgcc atgagaggca cttgaagtac gcctttattc
     2581 ttttgaaagc gcaccaccgc ttcattggga ttttcaggcg caagagtgta tgtcgcttgt
     2641 ggttttagaa ctttaccttt ctcgtcgaca aaagttaaat aaagcgttgc ttctttaggg
     2701 agggtgacta tgcgttcaag ttcaaaatct ttctgaatga ccacgggtga attgatgttc
     2761 cgcaaatcgg caatgtactc aacaggagta tacccgggag cggtaacgac cattttatat
     2821 tgttttttat tcacaaggtc tgcgctgtaa agcccgtctg tttcttgtgt tgaaaaatta
     2881 cgttcgccat ccacatcggt gatgcggatg ttcgcttcga tccctttgtc gctgaatttt
     2941 tctttgacgc ggcctttgta cgtcacgaca acctctggct ttgcaaagtc gggtacttta
     3001 gcgcggtaca cgtcaaaact accttggcca ttttcacggt tcgaggcgaa gtacatgtag
     3061 ttaccgttac ggactacggt ggggtattcg tcgtcgaagg tcgagttaaa gggtttgccc
     3121 aagttttgag ctttacgcca caaaacttcg ttttcgtcgg atgagcccac ttgcggcaag
     3181 ccttgtaatt tttg
//
LOCUS       DAOMPD010000313         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12340_length_3185_cov_3.077636, whole genome shotgun sequence.
ACCESSION   DAOMPD010000313 DAOMPD010000000
VERSION     DAOMPD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12340_length_3185_cov_3.077636"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..485
                     /locus_tag="PLY93_10435"
     CDS             57..485
                     /locus_tag="PLY93_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRP69937.1"
                     /translation="MYIKGKSFDEIQIGESASFSKTISETDVYLFAGISGDFNPLHVN
                     EEYAKISPFKTRIAHGGLAASLLANVLGMKLPGLGTVALEVTQKFRTPVYFGDTVTVT
                     VTVAKKVERMKIIEMNILWSNQRGETVSKGSCRVLPPTKD"
     gene            534..1433
                     /locus_tag="PLY93_10440"
     CDS             534..1433
                     /locus_tag="PLY93_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP69938.1"
                     /translation="MLKPPVLIIDPDIALQEILGEILRNRFRIFAAANIAAAKEVMQG
                     EEPDLVFIELQLPNESAIDFIEQTKRSKANTQFIVITDNATVENAISALRHGAFDFLK
                     KPFEIEDIARIVEKFFSITKNREVDYDIYSSIIAESRTFELPTNFALVSTFMSEIMHI
                     VSRFSGIDKKATLTIRLSLYEMLVNAMEHGNLEVDYEMKKKLLDEVVDYQRFLEERAK
                     QTSYNERKVLVSYHYDAKSISFTITDEGPGFDVSKVPNPHASENLESLNGRGIFISRV
                     NMTRVTYNDKGNSVTLYKELLKQ"
     gene            complement(1435..2388)
                     /gene="rsmH"
                     /locus_tag="PLY93_10445"
     CDS             complement(1435..2388)
                     /gene="rsmH"
                     /locus_tag="PLY93_10445"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRP69939.1"
                     /translation="MEATAEKTAAHVSILVDAVVESAAALKNLATDKRRVWDGTAGLG
                     GHLKALADTYPVAALFASDADAAMLELARARLGEKVREYRHANFSTNPFSDLTPFGFI
                     FLDLGISSAHFDFFERGFSFRYDQELDMRMDTTRGKGAVHVLATQSEEKLAQIFFDYG
                     EEKFARRIAREIVLRRRTAPVTKTFELTEICEKIYPPKYKAKGHAQRHAATRVFQALR
                     IYVNGELDALQKALQTLPQQLCVGGRLAIITFHSLEDRMVKHAFRRLSFIEQNDPHAK
                     SNYAEGDFKLLNAAGITPTDEEIEKNPRARSARLRVLERVR"
     gene            complement(2385..>3185)
                     /locus_tag="PLY93_10450"
     CDS             complement(2385..>3185)
                     /locus_tag="PLY93_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP69940.1"
                     /translation="GQLTEAIRRNPHSVLLFDEIEKAHPEIQNILLQVLDEGELTDNG
                     GHRVDFKETVIILTSNIGARFLQKGGKLGFSGEDQTKEETKREQVLEELRRHFSPEFL
                     NRIDEVITFDPLSKNEIQEIVDLTIEELNFNALTKNIFLSLKKNARTYLAEKGYSEKY
                     GARPLKRLVQREIEDELAMMLLEKKITEPVEIKISTHKKDGVEKLSFTLHNLSAEKFL
                     EIKNEFYEDEASIDEIWRNSPPEIEDEGKIENNEGEIENAIESEASKK"
BASE COUNT          820 a          706 c          755 g          904 t
ORIGIN      
        1 ggtatacgca ggacgaaatc aacgagctga aaaaacaaaa gtgtgtgtga ggctttatgt
       61 acattaaagg taaatcattc gatgaaatcc aaattggcga atcggccagt ttttctaaaa
      121 ccatcagcga aaccgacgtg tatctttttg cggggattag cggcgatttt aatcccttgc
      181 atgtcaacga agagtatgct aaaatatctc cttttaagac acgcatcgcc catggcggtc
      241 ttgccgcttc gcttttggcc aacgtgttag ggatgaagct acccggactg ggcacggttg
      301 cgcttgaggt gacacagaaa tttagaaccc ccgtgtattt cggcgatacg gtaaccgtga
      361 cggtgaccgt tgccaaaaaa gtagagcgga tgaaaattat cgaaatgaat atcctgtggt
      421 caaaccaacg cggtgaaacg gtgagtaagg gctcgtgtcg agttttaccg cccaccaaag
      481 attagcgctt tcggttgaca ctgtactttt tttagaataa gcgtatgcag tccatgttga
      541 agccaccggt cctgattatc gatcccgata ttgctttgca agaaattctg ggggagattc
      601 tacgtaatcg gtttcgtatt tttgcagcag cgaatattgc tgccgctaaa gaggtgatgc
      661 aaggtgaaga acccgatctg gtctttattg aattgcaatt acctaatgag agcgctatcg
      721 attttatcga acaaactaaa cgttcaaaag caaacacaca gtttattgtg attaccgaca
      781 atgcgacggt tgagaatgca atctcggcgt tgaggcatgg cgcattcgat ttcttaaaaa
      841 aaccttttga aatcgaagat attgcccgta tcgttgaaaa attcttttca atcactaaaa
      901 atcgtgaagt ggattatgat atctactcgt cgattatcgc agaatctcgg acatttgaat
      961 taccgacaaa ttttgcgttg gtttcgacgt tcatgtccga gattatgcac atcgtctcgc
     1021 gtttctcggg gattgacaaa aaagcgacgc tgacaatccg tctttcgctt tatgaaatgc
     1081 tcgtcaatgc gatggagcac ggtaatctcg aagttgatta tgaaatgaag aaaaagctat
     1141 tggatgaggt cgtcgattat cagcgttttt tagaagagcg cgcaaaacaa acgtcgtata
     1201 atgaacgcaa agtgctcgtg tcgtaccatt atgacgcaaa atcgatttca ttcacaatca
     1261 ccgatgaagg cccaggattt gatgtgagta aagtacctaa ccctcacgcg agtgaaaatc
     1321 tagaaagcct caacggacgc ggtattttta tttcgcgcgt gaacatgacg cgcgtgacct
     1381 acaacgacaa aggcaactct gttactttat ataaagagtt gttaaaacaa taaattagcg
     1441 aacgcgctct aaaacacgca accgcgccga gcgtgcgcgc ggattttttt cgatctcttc
     1501 gtccgtgggc gtaataccgg ccgcattcaa cagtttgaaa tccccctcgg cgtagttgct
     1561 cttcgcatgg ggatcgtttt gttcaatgaa agaaagccga cgaaaagcgt gtttcaccat
     1621 cctgtcctca agtgaatgga aagtgatgat cgccaaacga ccgcccacgc ataattgttg
     1681 cggcagcgtt tggagcgctt tttggagagc atcgagttcg ccgtttacgt aaatccgcaa
     1741 tgcttgaaaa acgcgcgtgg ctgcgtggcg ttgagcgtgt cctttcgctt tatatttcgg
     1801 cggataaatt ttttcacaaa tttccgtcag ctcgaaagtt tttgtgacgg gcgcggtacg
     1861 tctgcgcaaa acaatttcgc gtgcgatgcg acgggcaaat ttttcttcgc cataatcaaa
     1921 aaagatttgc gctaattttt cttcgctttg cgttgcaaga acgtgcaccg cgccttttcc
     1981 gcgtgtggtg tccatgcgca tatcaagctc ttggtcgtaa cgaaaactaa acccgcgttc
     2041 aaaaaaatca aaatgcgcag aggatatacc taaatctaaa aaaataaaac cgaacggggt
     2101 taaatctgaa aatgggtttg ttgaaaaatt agcatggcga tactcacgca ctttctcgcc
     2161 aaggcgtgcg cgcgccaact ccaacatggc ggcgtcggcg tcggaagcaa aaagcgcggc
     2221 aacggggtag gtgtcggcaa gcgcctttaa gtggcctccc aaaccagccg taccatccca
     2281 aacgcggcgt ttgtcggtgg cgagattttt tagagcggcg gcactttcga caacggcgtc
     2341 gacaagaata ctgacgtgtg ccgccgtttt ttcagcggtg gcttctattt tttcgatgcc
     2401 tcgctttcga tggcgttctc tatttcacct tcgttatttt caatttttcc ttcgtcttca
     2461 atttcagggg gagagtttct ccaaatttcg tcgatggacg cttcgtcttc gtagaattca
     2521 tttttgattt ctaagaactt ttctgcactc aaattgtgca atgtaaaaga aagtttctca
     2581 acaccgtcct ttttgtgggt cgaaattttt atttcaactg gctcggtgat ttttttctca
     2641 agtaacatca ttgccagttc gtcttcaatc tcgcgttgga ccaatctctt taaggggcgt
     2701 gcgccgtatt tttcgctata ccctttttcc gccaggtatg tgcgcgcatt cttcttgaga
     2761 gacaaaaaaa tatttttcgt gagtgcatta aagttgagtt cttcgatggt caaatcgacg
     2821 atttcttgaa tctcattttt ggaaagtgga tcgaatgtaa tcacttcgtc gatacggttc
     2881 aaaaattcag gagagaagtg gcggcgcaac tcttcgagca cttgttcacg cttcgtttct
     2941 tccttggttt gatcttcgcc gctgaagccc agcttgcccc ctttttgcaa aaagcgcgcg
     3001 ccgatgttgg aggtgaggat gattaccgtc tctttgaaat ccacgcggtg gccgccgttg
     3061 tcggtgagtt cgccctcgtc gagcacctgc aacaaaatgt tttgaatctc ggggtgtgct
     3121 ttttcaattt cgtcgaagag taacaccgag tgcgggttac gccgaattgc ttcggtgagt
     3181 tgacc
//
LOCUS       DAOMPD010000314         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12371_length_3177_cov_2.593209, whole genome shotgun sequence.
ACCESSION   DAOMPD010000314 DAOMPD010000000
VERSION     DAOMPD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12371_length_3177_cov_2.593209"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>619)
                     /locus_tag="PLY93_10455"
     CDS             complement(<1..>619)
                     /locus_tag="PLY93_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP69941.1"
                     /translation="PLVNVTPGGEQWAPANYNSGFSGYSLAYRALAASINLVSVQIYD
                     LVGPDAIINYISRLTKAPESRFQPNPALSLGASELTPAELLLGYAIIGNKGREIIPHA
                     IIYIADRDGNVIAHPENDVIQALNFKRRNGQIQVVEEGPAFILRKMMENVVNAGTPTQ
                     AIRIDAGYKGAGAGKTGTSNGFNDAWFGGYTTDMAAVIWFGMDNGN"
     assembly_gap    620..629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<630..2105)
                     /locus_tag="PLY93_10460"
     CDS             complement(<630..2105)
                     /locus_tag="PLY93_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRP69942.1"
                     /translation="MQLYENEVNPPLKSLPWWGKVEKIAVYLFFITALLGGLFLGFIH
                     FRIHSKTGDLDRLASFRPTIPTRLYDQKGRLIAELFRHQRKLVTLEEIPRPVIAAFLA
                     IEDSNFYEHFGIDFKGILRAALKNLRAGGVVAGGSTITQQLVKGLYTDSERTFYRKIY
                     EAVLALQVEKAFTKDEILEMYFNQTYFGHGAYGIASAAQFYFDKPVSKLNFMEASILA
                     ALPKSPHNYSPFRAPHEAYKKNRVSLNRLVEVGYITKKEADDLYNTFWPIYWKKIVTT
                     PPSKTIFGEKINNAPYFTEHVRQELIALYGEDVVYSKGLQVYTTLDLDHQKSAEKALL
                     PELEKLDPIARNQNKYTSGGIDGSLLSTYFALGNIVGIPGIRREYSLRNDFRKKFKED
                     AADAFELVSLVLPARTSNEVSSEFMSANREHRSDLRVQGAFLALDMKTGRYTAMIGGR
                     EFKSSDQFNRATMARRQPGSAIKPFVYGAALEARAIHYAMGF"
     gene            complement(2200..>3177)
                     /locus_tag="PLY93_10465"
     CDS             complement(2200..>3177)
                     /locus_tag="PLY93_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP69943.1"
                     /translation="NDILDFSLMEAGKLTLHSTPFSLVEFYAEISQITEVLAQQKKIY
                     FTVKPLPEGLPEKVLADEARLKQALLNVIGNAVKFTAKGFIKISVHWIETRSDTVSLE
                     FVVQDTGVGILDSDLPRLFKPYMQADQKRTRRFGGTGLGLAITASIVEAFGGKIAVQS
                     TKGVGSWFSLQIPLKIAPKEKKPQFPSAVQNESTQKIADSYPLHILVVEDEPVNQIVI
                     TESLRLLGYEARVVSSGFEAINLVEKEDFDLILMDIQMPALSGIEATERILQLPERAH
                     CKIIAVTADTSNENRNQCAAAGMVDFLMKPYTLPALREVILKWAKKNHL"
BASE COUNT          774 a          718 c          811 g          864 t
ORIGIN      
        1 tgttaccgtt atccatgcca aaccagatca ccgctgccat gtcggtggtg tatccgccaa
       61 accacgcgtc gttaaaaccg ttgctcgttc cagttttccc ggcgcctgca cctttgtaac
      121 cggcgtcgat acgaatcgct tgcgtgggtg tccccgcatt gacgacgttc tccatcattt
      181 tgcgtaagat aaaagcaggg ccttcttcga cgacttgaat ttgcccgttg cggcgtttga
      241 aattgagcgc ttgtatcacg tcgttctcag ggtgtgcaat cacgttaccg tctctatcgg
      301 caatataaat gattgcgtga ggtataatct cgcgtccttt attgccaatg attgcatacc
      361 ccaacaaaag ttctgcgggt gtaagttcgc tcgcgcccaa agaaagtgcg gggttgggtt
      421 gaaaacgcga ctcgggcgct ttggtgaggc ggctgatata gttaataatc gcatcggggc
      481 caacaaggtc gtaaatttgc accgatacga ggttgatcga tgcggcgagc gcacggtaag
      541 ctaacgaata accgctaaat ccggagttgt agtttgccgg tgcccattgt tcgcctccgg
      601 gtgtgacgtt taccaaaggn nnnnnnnnng aaccccatag cgtagtggat ggctcgtgct
      661 tcgagtgccg cgccgtacac aaacggtttg attgccgaac cgggctggcg gcgcgccatt
      721 gtagctctgt tgaattggtc ggatgattta aactcgcgcc cgccgatcat cgcggtgtag
      781 cggccggttt tcatatcgag cgcgaggaac gcgccttgca cgcgtaaatc ggagcggtgt
      841 tcgcggttgg ccgacataaa ctcgctcgac acttcgtttg aggtgcgcgc gggtaagaca
      901 agggagacaa gctcgaacgc gtcggcggcg tcttctttga attttttacg gaagtcattg
      961 cggagcgaat actcgcggcg gataccgggg atacccacaa tgttgcccaa tgcaaagtac
     1021 gtggagagga gcgaaccatc gataccaccc gatgtatatt tattttggtt acgtgcgatg
     1081 gggtcgagtt tttcgagttc gggcaggagc gctttttctg ccgatttttg gtgatctaag
     1141 tcgagagtag tgtaaacttg gagccccttg gagtatacca cgtcttcacc ataaagcgcg
     1201 atcaactctt ggcgcacatg ctcggtgaag taaggggcat tattaatttt ctcgccaaaa
     1261 atcgttttag aaggtggggt ggtaacaatc tttttccaat agataggcca aaatgtgtta
     1321 tagaggtcgt ccgcctcttt tttggtgata taccccactt ccactaaacg gttgagtgag
     1381 actcgatttt ttttgtatgc ttcgtgggga gcgcggaaag gggaatagtt atgcggcgat
     1441 ttaggcagtg cggctaaaat tgacgcttcc ataaaattaa gttttgagac gggtttatcg
     1501 aaataaaact gtgccgccga ggcgataccg tatgcgccat ggccaaaata agtttggttg
     1561 aaatacattt cgagaatttc gtctttagtg aacgctttct ctacttgcag tgccaacacc
     1621 gcttcgtaaa tttttcgata gaacgtgcgt tcgctatcgg tatagagtcc tttcacgagc
     1681 tgctgcgtga ttgttgaacc gccggcaacg acaccaccgg cgcgtaaatt tttgagcgcc
     1741 gcgcgcaaaa tgcctttgaa gtcgatacca aagtgttcgt aaaaattcga gtcttcaatc
     1801 gccaaaaatg ccgcaatcac cggacggggg atttcttcga gagtcactag tttgcgttgg
     1861 tggcggaaga gttcggcaat gagtcgccct ttttgatcat aaaggcgtgt ggggattgtg
     1921 gggcgaaaag acgccaagcg gtctaaatcc cctgttttag agtggatgcg aaaatgaata
     1981 aagcctaaaa agagtccacc taaaagagcg gtaataaaga agagatacac cgcaattttt
     2041 tcaactttgc cccaccatgg caacgacttc aaaggggggt tcacttcgtt ctcatataac
     2101 tgcacgggtg attttcttct ttagatatac acggaaaaga attttcgtaa gcttaaggtg
     2161 ggctaagaat ctgtgcgcca acttgctaag cgagtaggct cataaatgat ttttttttgc
     2221 ccacttgaga atcacctcac gtagcgcggg taatgtgtag ggtttcatga gaaaatcgac
     2281 catacctgct gcagcgcatt ggttgcggtt ttcgttcgag gtgtcggcgg tcacagcaat
     2341 aattttacaa tgtgcacgtt cgggtaactg taaaatacgc tcggtggctt caattcccga
     2401 aagagcgggc atttgaatat ccatgagaat aagatcaaag tcctcttttt ccactaagtt
     2461 gatagcctca aatccactcg acacaacacg cgcttcgtac cctaaaaggc gtaacgactc
     2521 ggtgatgaca atctggttga ccggctcgtc ttcaacgact agaatatgta acggataact
     2581 atcggcaatt ttttgagtag attcattttg aacggcagac gggaactgcg gttttttttc
     2641 tttcggagct atttttaagg ggatttgcaa ggaaaaccaa ctgccgacgc cttttgtact
     2701 ttgtactgct attttgccac caaacgcttc aacgatgctc gctgtgatgg caagccccag
     2761 accggtgcca ccaaatcgcc gcgtcctttt ttggtcggct tgcatgtatg gtttaaataa
     2821 tcgaggcaaa tcggagtcga gtatacccac acccgtatct tgcacgacaa actcgagtga
     2881 caccgtgtcg ctccttgttt caatccagtg cacagatatt ttaatgaaac cctttgcagt
     2941 gaattttact gcgttaccaa tgacgttgag taatgcctgt tttaggcgcg cttcgtcggc
     3001 gagtaccttc tctggaagtc cttcgggtaa aggcttcacc gtgaagtaaa tctttttttg
     3061 ttgcgctagc acttcggtaa tttgtgaaat ttcagcatag aactcaacta aagaaaacgg
     3121 tgtagagtgt aacgtcagtt tacccgcttc cataagagag aaatctaaaa tgtcgtt
//
LOCUS       DAOMPD010000315         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12521_length_3146_cov_2.183112, whole genome shotgun sequence.
ACCESSION   DAOMPD010000315 DAOMPD010000000
VERSION     DAOMPD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12521_length_3146_cov_2.183112"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLY93_10470"
     CDS             <1..324
                     /locus_tag="PLY93_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69944.1"
                     /translation="VVAWFKDKKNKARLKKLLENGVQITDATATRISNAFEGKTFVFT
                     GTLTHFTRESAESAVKERGGRASSSVSKRTDFVVVGTDAGSKEKKAKELGVKILTEEE
                     FKLML"
     gene            complement(326..775)
                     /locus_tag="PLY93_10475"
     CDS             complement(326..775)
                     /locus_tag="PLY93_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69945.1"
                     /translation="MKRPTCFKVVYLGVLTFTFTFCQKNHELPEGARLTPMKEYTVDN
                     AREWTPIAKEHVPTAKRSINKGKPALIIEVPLRSADISHYIEKIGILGADGKEIAVEA
                     LPRSATPTLHAYFDLKLLPWSGKIKIFAKCNKHDLWVSEIKVKDLGL"
     gene            complement(798..1208)
                     /locus_tag="PLY93_10480"
     CDS             complement(798..1208)
                     /locus_tag="PLY93_10480"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRP69946.1"
                     /translation="MKVLRRQKNLSFGTSPDNEQGLKLELRFDEDTKTAYGEIVLPVH
                     FQGNQPDCVHPGVIALMLDEVMMKINQALNLNVTTGEVTVRYLQLAKVDEPLYLRGYF
                     VKKNKRVIENRAELEDDIGKIVARGKGKYIELDL"
     gene            1360..2223
                     /locus_tag="PLY93_10485"
     CDS             1360..2223
                     /locus_tag="PLY93_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRP69947.1"
                     /translation="MKNDFTLDPTQTNKDDSDPILEYKNIDTRLIESRKVMLGKIPFY
                     QVGSVDLVAHSIHCLDAMREKKAKSTGQFGPQLVFPFDPYRYVWVRFRKRMLAMANSS
                     FINLPDGAGMLYMSRSLHEPLPELISLVGYAMNLIRIAHAKEYTVFLLGSHDEVVEKL
                     YINFRRSFQGLRIVGRHNGYLKGAVGQRVLEAIRKTDPHILLVGMGYHKGMRWIEENR
                     KNLGDLIVVNVGGAFETLAGTKTKAPAFMAGNGYTWLWRALNRPYRWHRLFLVVYWFL
                     ETTYFRFFKKK"
     gene            complement(2210..3028)
                     /locus_tag="PLY93_10490"
     CDS             complement(2210..3028)
                     /locus_tag="PLY93_10490"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRP69948.1"
                     /translation="MDNYALEHQTLLPVIEDAGRAVMRYFQEHDYTIQHKDADNPVTD
                     ADFAANRIIRAALTKKFPHDAVLSEESENSSERQRMEERLTKNRVWIIDPIDGTREFI
                     KGRPHFAISVGLVVHGVPVLGFIHNPAQQYMLSGGMSMGLFKNGTAFTPPERFGVNPT
                     QALPNIIVSRSEYTHGHLPHLAKWYHNLESHTMGSIAYKLALVADGTYDLTVSVKPKN
                     EWDIAGGAALLKAARLILLDGAFNDFIFNKPQTESLGLVAGTPEACAWYRTIFS"
BASE COUNT          738 a          706 c          840 g          862 t
ORIGIN      
        1 gtggttgcat ggtttaaaga caagaaaaac aaagcgcgtt tgaagaagtt gttggaaaat
       61 ggcgtgcaaa ttaccgacgc taccgccacg cgaatctcca atgctttcga gggtaaaacg
      121 tttgtgttca ccggcacgct tacgcacttc acgcgcgaga gcgccgagag cgcggtgaaa
      181 gagcgcgggg gacgggcgtc gtcgtcggtg agcaaaagaa ccgattttgt ggtggtggga
      241 accgacgcgg gcagcaaaga aaaaaaagcg aaagagttgg gtgtgaaaat tttaaccgaa
      301 gaagagttta agttaatgct gtaatttaca gccccagatc tttgactttg atttcactca
      361 cccataaatc gtgtttattg cacttggcaa aaattttgat tttccccgac caaggtaata
      421 gtttcaaatc aaaataagcg tggagagtgg gggtagcgct gcggggtaat gcctcaacgg
      481 cgatttcttt accgtcggcg ccgaggatac caattttttc aatgtaatgc gatatatctg
      541 cgcttctgag tggcacttcg ataataagtg cgggctttcc tttgtttatc gaacgcttgg
      601 ctgtcggcac atgttctttt gcaatgggtg tccactcgcg cgcattatcg acggtgtact
      661 ctttcatggg cgtgagacgc gcaccttcgg gtaattcgtg atttttttgg cagaacgtaa
      721 atgtaaaagt aagtacgccc aagtacacaa ctttgaagca agtagggcgt ttcatggttg
      781 atttaatcta cacgccgtta aaggtctagc tcgatgtatt tgcctttacc acgcgctacg
      841 attttaccga tgtcgtcttc cagttcagcg cggttttcga tgacgcgttt attttttttc
      901 acaaaataac cgcgcaagta aaggggttcg tccaccttgg cgagttgtaa ataccgcacg
      961 gtgacctcgc cggtggttac gttcaggttt aacgcttggt tgattttcat catcacttcg
     1021 tcgagcatga gagcaataac gcccggatgg acgcaatcgg gttggttacc ttgaaaatgg
     1081 acgggtaaca caatttcgcc gtaagcggtc ttagtatctt cgtcaaagcg tagttcgagc
     1141 ttcaagccct gctcgttgtc aggactggta ccgaaactca ggtttttctg tcttctcagt
     1201 accttcataa gcacatgtta ccttttgtaa cacattgtca agcactatta agtgtatgaa
     1261 gttgacttca gttaacttct ccaaactact gaaacttgct ttacagttag cgcacaggct
     1321 ctaattggtt gaactctttt ataatgcacc gcagagacca tgaaaaacga ttttacttta
     1381 gatcccactc aaaccaataa agacgattct gacccgattt tagagtacaa gaacatcgac
     1441 acccgcttga tcgagagccg caaggtaatg ttggggaaaa ttccctttta ccaagtgggc
     1501 tctgtggatt tggtggcgca ttccatccac tgtttggatg cgatgcgcga gaaaaaagca
     1561 aagtccactg gccagtttgg gcctcagctt gtttttcctt ttgatcccta ccgttatgtg
     1621 tgggtacgtt tccgcaagcg tatgcttgcg atggcgaatt cttcttttat taacttaccc
     1681 gacggcgcgg ggatgctcta tatgtcgcgc tcgctacacg agccgctgcc cgaacttatt
     1741 tcgctggtgg gttatgcgat gaacctcatc cgtattgcac atgcgaaaga gtacaccgtg
     1801 ttcttgttgg gctcgcacga cgaagtggta gaaaaacttt atatcaattt tcgtcgttcg
     1861 ttccaaggtt tgcgcattgt cgggcggcat aacggctatc tcaaaggcgc agttggccag
     1921 cgtgttttgg aagcaatccg taaaacagac ccacacattc ttttggtggg gatggggtat
     1981 cataaaggca tgcgctggat tgaggagaat cgaaaaaatt tgggcgacct cattgtggtg
     2041 aacgtcggcg gtgcgtttga aaccctcgct ggcacaaaaa caaaagcgcc ggcgtttatg
     2101 gcgggtaatg gctacacctg gctctggcgt gcgttgaacc gcccttaccg ttggcatcgc
     2161 cttttcttgg tagtgtattg gtttttggag acgacgtact tccgtttttt caagaaaaaa
     2221 tagtacggta ccaagcgcat gcctctggtg tgccagcgac gagccccagc gattcggttt
     2281 ggggcttatt aaaaataaaa tcgttaaaag caccatcgag aagaatgagc ctcgctgctt
     2341 ttaataacgc ggcaccaccg gcaatatccc actcattttt gggcttcact gaaacggtta
     2401 aatcgtacgt accgtcggcg acgagcgcca acttgtacgc gatggaaccc atcgtgtgcg
     2461 attccaaatt gtggtaccat tttgcaagat gcggtaagtg gccgtgtgtg tactcgctgc
     2521 gtgaaacaat gatgttgggt aacgcctgag tgggatttac cccgaatcgt tccggcggcg
     2581 tgaacgcagt gccgtttttg aaaagtccca tcgacatgcc gccgctcaac atgtattgtt
     2641 gcgcggggtt gtggataaaa cccaagaccg gcacgccgtg gacgacaagc cccaccgaga
     2701 tggcaaagtg gggtctgcct ttgataaatt cacgcgtgcc gtcaatgggg tcgataatcc
     2761 acacacggtt tttggtgagc cgctcttcca tgcgctggcg ttcagagctg ttttcgcttt
     2821 cttcgctcaa aacagcgtcg tgcgggaatt tttttgtgag cgcagcgcgg atgatacggt
     2881 tggcggcaaa gtcagcgtcg gtgacggggt tgtcggcgtc tttgtgttgg atggtatagt
     2941 cgtgttcttg aaagtagcgc atcaccgcgc ggccggcatc ttctatgacg gggaggagag
     3001 tctggtgttc gagcgcatag ttatccattt ttttcctcga gtgcggggtt gaggagcctt
     3061 tgtggatcaa aagcttcttt aattatgtct tgcatcgcga aataactggg ggggagtgtt
     3121 gcgcgggcga ggtggcgttt gagcct
//
LOCUS       DAOMPD010000316         3119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12654_length_3119_cov_2.610966, whole genome shotgun sequence.
ACCESSION   DAOMPD010000316 DAOMPD010000000
VERSION     DAOMPD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_3119_cov_2.610966"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLY93_10495"
     CDS             <1..546
                     /locus_tag="PLY93_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP69949.1"
                     /translation="LLDDIFQKVPVNTEGMLCIHESDPGLFLGYWQERGSFIRPIQNG
                     WFLTGDYAWQNAEGYIFFMGRRDDILKSFGYRVSPFEIERVFRDHPSIEEVVAFGEKF
                     NDEKMLIALAVKLKVGYTLNEAEILSWAEDKLARYKMPKKIYFRDSFPRSINGKIVRS
                     KLVKDSGILEVAARKNAEMPA"
     gene            complement(749..>3119)
                     /locus_tag="PLY93_10500"
     CDS             complement(749..>3119)
                     /locus_tag="PLY93_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRP69950.1"
                     /translation="RLGGVGGWEQDLITGDFWYSQEMLRILDLPADTTMRPSETVQFI
                     DEADRAAHEARIQLALEEKTIWESEFATTTQKGRHVIVRSLGQPIFENGHLVKMVGFV
                     QDITEERESDRLIKETNERLSLIFDTLSEGVSLSELVRDEKGNIIDHRILEVNNAFYR
                     IADYAKNLPVIGGLASELFQIPREFFTQFWAKHINSKEPTQIEYHSPSSGNHLIVTTS
                     PLQNNQFITSFYDITVRKKQELEIKESEERHRKLIESIADAVFVHRDGVIQYANPSAS
                     LLLGAVSTQELVGRNVRDIVPLENRNAVLKQSPLQEAQIIRLNGASVAVEMSATEITF
                     SGHDAILLVARDLSERKRAEEKIRYMGQHDQLTQLPNRLLLTDRILQAITFAEFHPTV
                     FAVLVLDVDHFKKINDALGHHKGDLFLQEVAARLLTVVKSIDTLSRQGGDEFLILLND
                     LTEAKDAAQVAQKISQLFQTPFQIAESSITASTSIGIATYPHDGRDPETLIRNADIAM
                     YHAKDSGRNQFQFYSSDLNRITHERLELETLLRTAIQKEELTVHYQPQQNLSSGEIES
                     CEALLRWPHPERGFIPPGRFIPIAEETGLITEIGRWVLQKVAEDYKFFSDAGFDSLRI
                     SINASAKEIQDANFRSHIRHTVTTGVIPPSVLELEVTESMIMHDTAQAVRTIQELSDL
                     GIRFSIDDFGTGYSSLSYLRELKIHNLKIDQSFVRDLTDDPDDAAIVRTIIGLAKNLR
                     LQVIAEGVETEAQKRFLKVHGCDIIQGYELSPALSANDFVEFLQGKAVG"
BASE COUNT          800 a          663 c          755 g          901 t
ORIGIN      
        1 cttttagacg atatattcca aaaagtgcct gtcaacaccg aagggatgct ctgcatccac
       61 gaaagtgacc cagggctttt tctgggctat tggcaggaaa ggggaagttt tatccgcccc
      121 atccagaatg gctggttttt aaccggcgat tatgcatggc agaatgcaga aggatatatt
      181 tttttcatgg gacgccgtga cgatattctt aaatcattcg gataccgtgt gtcgcctttt
      241 gaaatcgaac gcgtttttcg tgaccatcct tccatcgaag aggtggttgc gttcggcgaa
      301 aaattcaacg atgaaaagat gttaatcgcc cttgcggtga aactcaaagt ggggtatact
      361 cttaatgaag cagaaatttt gtcgtgggct gaggataaac tggcacgcta taaaatgccg
      421 aagaaaattt attttcgaga cagtttccca cgctcgatta acggtaaaat tgtgcgtagc
      481 aagctcgtga aggatagtgg tattctggaa gtggccgcgc gaaaaaacgc tgagatgccc
      541 gcttgaaagt aggtttctct tcaaattgta cccttgtttt tgaccactcc agcacttggg
      601 gcggatagtt accctctaat gcgacataat tcgggaaacg cggatagtta ccccttaatg
      661 cgacataatc cgaaaaattt gaataattct tcccgcagtt aacagagtga tgttaatgct
      721 acacacgatg gatgaattgt tgattttttt aacccaccgc tttcccttgc aaaaactcga
      781 caaaatcgtt tgcagacaaa gcgggggata gctcatatcc ttggatgatg tcgcatccgt
      841 gcactttgag gaatcttttt tgcgcttcgg tttcgacacc ctcggcgatg acttgaaggc
      901 gcaagttttt agcgaggccg ataatcgtgc gcacaatcgc ggcgtcgtcg gggtcgtccg
      961 tgaggtcgcg tacaaacgac tggtcaattt tcaaattatg aatttttagt tcgcgcaagt
     1021 aacttagcga tgaatatcct gtaccaaaat catcgatgga gaaacggata cctaaatcgg
     1081 aaagctcttg aatggtgcgc accgcttgtg ctgtgtcgtg cataatcata ctttcggtga
     1141 cttccaattc caataccgac ggtggaataa cccctgttgt aacggtgtgg cggatgtggc
     1201 tacgaaaatt ggcgtcttga atttcttttg cgctggcgtt tatggaaata cgtaaactat
     1261 caaaacccgc atccgagaaa aatttgtaat cctcggcaac tttctgcaaa acccagcggc
     1321 caatttcagt gatgagaccg gtctcttcag cgatggggat aaatcgtccc ggtggaataa
     1381 aaccccgttc ggggtgtggc catcgtagga gtgcttcgca cgattcaatt tcgccgctac
     1441 ttaaattttg ctgcggttga taatgcacag tgagttcttc tttttggatt gccgtgcgta
     1501 acaaagtttc gagttctaac cgctcgtgtg taattcggtt gagatcgctg gaataaaact
     1561 gaaattgatt acggccacta tctttggcat ggtacatggc gatgtcggcg ttacggatga
     1621 gcgtttcagg gtctcgacca tcgtgagggt acgttgcaat gccgatgctc gtcgacgcag
     1681 taatcgacga ttcggcaatc tggaaaggtg tttgaaaaag ctgacttatt ttttgcgcaa
     1741 cttgtgcggc gtcttttgct tcggttaaat cgttaaggag gattaggaat tcatctcctc
     1801 cttgtcgaga gagggtatcg atacttttta caactgtcaa caggcgcgca gcgacttctt
     1861 gcaagaataa atcccccttg tgatgcccca gtgcgtcgtt aatttttttg aaatgatcga
     1921 cgtctaaaac aagcaccgcg aataccgttg ggtggaattc ggcaaaagta atggcttgta
     1981 gaatgcggtc ggtcagaaga agacggttcg ggagttgagt gagttgatcg tgttgtccca
     2041 tataacgaat tttttcttcg gcgcgtttgc gctcggaaag atcgcgggca actaagagta
     2101 ttgcatcgtg tccggaaaaa gtaatttcgg ttgcagacat ttcaaccgca accgacgcac
     2161 cgtttaagcg gatgatttgt gcttcttgca gtggagattg tttcaagact gcgttgcgat
     2221 tttctaaagg cacaatatcg cgcacatttc tacccacgag ttcttgggtt gataccgcac
     2281 ccaagagcag tgatgcgctg gggttggcat actggatgac accgtcacgg tgcacgaata
     2341 ccgcgtcggc aatcgattca atgagttttc ggtggcgctc ttcgctttct ttaatttcta
     2401 gttcttgttt tttacgaaca gtaatgtcgt aaaacgaagt gataaattga ttgttctgca
     2461 acggtgaagt agtgacaata agatgattgc cactacttgg actgtggtat tctatttgag
     2521 tcggttcttt tgagtttata tgttttgccc aaaattgcgt aaagaattcg cgtggtattt
     2581 gaaaaagttc gctggctaac ccaccaataa cgggtaaatt tttcgcataa tcggcaatgc
     2641 gataaaaagc attattgact tcgaggatac ggtggtcgat gatattccct ttttcatcgc
     2701 ggactaattc ggataaagaa acgccttcag aaagtgtatc aaaaattaac gaaagccgtt
     2761 cgttggtttc tttaatcagt cgatcacttt ctctttcttc agtgatatct tgtacaaaac
     2821 caaccatctt aacgagatgc ccgttttcaa aaataggttg cccaagagag cgtactatga
     2881 catgcctgcc tttctgtgtc gtggtggcaa actcagattc ccatatcgtt ttctcttcta
     2941 atgctaattg aatacgcgct tcgtgtgcgg cacgatctgc ttcgtcgata aattgcaccg
     3001 tttcactggg gcgcattgta gtgtctgcgg gtaaatcaag aatcctcaac atttcttgcg
     3061 aataccaaaa atcgccggtg ataagatctt gctcccaacc gccaacacca ccgaggcgg
//
LOCUS       DAOMPD010000317         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12746_length_3102_cov_2.991139, whole genome shotgun sequence.
ACCESSION   DAOMPD010000317 DAOMPD010000000
VERSION     DAOMPD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12746_length_3102_cov_2.991139"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLY93_10505"
     CDS             complement(<1..654)
                     /locus_tag="PLY93_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP69951.1"
                     /translation="MKSFFTMAICKKKQQVPETKTRDTILAAAVEEFAQNGFAGARTE
                     NIAKAANVNKAMLHYYFQDKETLYDAVLDTLYAWLLETETLTQEVAITPMNSVQFVHL
                     FLKIVVHKHANPHNEAFRRILAWELAAGQNNFKRVAQKYIVPRILQLKQVLEVGIANG
                     ELQCKNPILTLYSMVSQVVFYYMHRYTYEDTPIHDELYSNVTPNYFLSFLLENFIACY
                     "
     gene            complement(717..2240)
                     /locus_tag="PLY93_10510"
     CDS             complement(717..2240)
                     /locus_tag="PLY93_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRP69952.1"
                     /translation="MLSILAGLLLLGICVFFHELGHFLIGKWMGVRPRVFSIGYGRAL
                     FFRRRKSTIYQITAIPLGGYVQFHGDDPTKQNRKMRKGDFFSVGPWRRMALAFGGPLF
                     SILLGFVVVFILLQTGYRPMDNIVRVDEKSALYKAGLRDGDRVTSIDGNKTYSFDRVL
                     YEVGFAKGDTIDTTVERGGKTLALKLPVDRVEVGTPGLFTGARPFSRLTMTENGPQGV
                     DLKTGDEILKANGTEVYSIEELIQVLNTEKKDTATLEIRRPDAQRGFTLLTIQVPLKK
                     VPRVIFKNIVDLQTKKSIPELAIEEASGKAFQNIFINGESFATFIEFRNAIEKANAAA
                     LQLRIGPVEVSANVELSHRRLIGIRPAEKRINNATELSFIQKIMYSADETIFITKSTL
                     VGLGRIIQGKLSFNKSVSGPVKMVAIAAKTVEMGWQTYFLLLAQITIILGVMNLLPIP
                     VLDGGHILFYLIEALYKPLSFKVQATATRVGMTLMLAMGIYVIGLDIFDVFVRRVFG"
     gene            2331..>3102
                     /locus_tag="PLY93_10515"
     CDS             2331..>3102
                     /locus_tag="PLY93_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69953.1"
                     /translation="MAKKLFFHRRHKLPPWAQIYSVWGVLAAVTLVLLFFTSIEQVAP
                     TKTSVAPTSARAFSQTLFYDLREADSNESFDVLYTYAKQLASRGNFKLTQEKKADCAI
                     VRLTRGSDDKHSLTAAVTGDKTQAAFHLLSFLEYASAHHSDAAFDASLIFTSARCSLE
                     KAMLQIERETNAPLVELNIGSADTHYNRIGALRNLQARTFFESAFLSSERTLWANIMA
                     TTAADNEAVFQLPVKATWNKNPDAQVAENPLWHHPALAK"
BASE COUNT          836 a          751 c          693 g          822 t
ORIGIN      
        1 ataacaggcg ataaaatttt ctaacaaaaa cgacaaaaaa taattgggtg tgacattgct
       61 gtaaagctcg tcgtggatgg gtgtatcttc gtatgtgtag cgatgcatgt aatagaacac
      121 cacttgcgaa accatgctgt agagagtcaa aatcggattt ttacactgca attcgccgtt
      181 agcgatgcct acttcgagca cttgctttag ttgcaaaatt cgagggacga tatatttttg
      241 cgccacccgc ttaaaattat tttgaccggc ggcgagctcc cacgctaaaa tacgacggaa
      301 tgcttcgttg tgcgggttcg cgtgtttgtg gacaacaatt ttcaaaaaaa gatgcacgaa
      361 ctgcaccgag ttcatcggtg tgatcgccac ttcttgagta agtgtttccg tttccaaaag
      421 ccatgcgtat aaggtgtcca acactgcatc gtagagagtt tctttatctt gaaaataata
      481 atgcaacatc gccttattga cgtttgccgc cttggcaata ttttccgtgc gtgcgcctgc
      541 aaagccgttt tgagcaaatt cttcaaccgc cgcagctaaa atagtgtcgc gtgttttagt
      601 ttcaggaacc tgttgttttt ttttgcagat tgccattgta aaaaaactct tcaataacaa
      661 tatacaacat gttcaacttt tcgtctattt tattaaccat ttggttaatt ccaccctcac
      721 ccaaacactc gacgcacgaa cacgtcgaaa atatccaacc caatcacata aatccccatc
      781 gccagcatga gcgtcatgcc gacgcgcgtg gcggtagctt ggactttgaa agaaagcggt
      841 ttgtagagtg cttcgatgag ataaaataaa atgtgcccac cgtctaaaac ggggatgggc
      901 aaaagattca tcactcctaa aataatcgtg atttgcgcca agagtaaaaa gtacgtttgc
      961 caacccattt cgacggtttt cgcggcgatc gcgaccatct tcaccggccc agaaacgctt
     1021 ttgttgaacg aaagcttacc ttggatgata cgccccaacc ccaccagagt tgactttgtg
     1081 atgaaaatcg tttcgtctgc gctatacata attttttgta taaacgaaag ctcggttgca
     1141 ttgttgatac gcttttctgc ggggcggata ccgatgaggc ggcggtgcga tagttcgaca
     1201 ttggcgctca cctcgaccgg tccaatacgc agttgcagcg ccgcagcatt tgccttttct
     1261 atcgcattac gaaattcgat aaacgttgca aagctttcac cattgatgaa aatattttgg
     1321 aaggctttac cgctcgcctc ttcgatggcc aactcgggga tagatttttt agtctgtaag
     1381 tcgacaatgt ttttaaaaat gacacgcggc acttttttca gcggcacctg aatcgtgaga
     1441 agggtaaatc ccctttgcgc atcggggcgg cgaatttcga gcgttgcggt atctttcttt
     1501 tctgtattta agacttggat caactcttca atcgaataga cttcggtgcc gttggcttta
     1561 agaatttcat cgcccgtttt taagtccacg ccttgggggc cattctcggt catggtgagg
     1621 cgcgagaatg ggcgtgcacc ggtgaaaaga cctggagtgc ccacctctac acggtcgacg
     1681 ggtaatttta atgcaagcgt tttaccaccg cgctcaacag tggtatcaat ggtgtcgcct
     1741 ttggcaaagc ccacttcgta aagcacgcga tcgaaggaat atgttttgtt gccatcgatg
     1801 gatgtaacac ggtcgccatc tctcagacct gctttataga gtgcagattt ttcatccacg
     1861 cggacaatgt tatccatggg tcggtagccg gtttgcaata aaatgaacac cacaacaaaa
     1921 ccaagcaaaa tagagaagag tggaccgccg aacgccaacg ccatacgccg ccaagggcca
     1981 accgaaaaga aatcgccttt gcgcatttta cgattttgtt tagtagggtc gtcgccatgg
     2041 aactgcacat agccgcctaa aggtattgca gtgatttgat aaatggtact tttgcggcgg
     2101 cgaaaaaaga gcgcacgacc atagccaatc gagaacacac ggggacgcac ccccatccat
     2161 ttgccgatga ggaaatgccc cagctcgtga aaaaaaacac agatacccag gagtaataaa
     2221 cctgctaata ttgaaagcac acttcaattt aataaaatgg agaggcgttg gaaagtaaaa
     2281 ctttcatccg attgacgtgc gcacttacaa atttttctct acctttagct atggcgaaaa
     2341 agctattttt tcatcggcgc cataaacttc ccccttgggc acaaatttat tctgtatggg
     2401 gtgttcttgc cgccgttact ctggttcttc ttttttttac ttcgattgaa caagtggcgc
     2461 caaccaaaac cagtgtcgct cccacttctg cacgcgcttt ttctcaaacg ttattttacg
     2521 atttgcgcga ggctgatagc aacgaaagct tcgacgtact ctatacgtat gcaaaacagt
     2581 tagccagccg cgggaatttt aaacttacac aggaaaaaaa agccgattgc gccatcgtgc
     2641 gtcttacacg aggctctgac gacaagcact cactcaccgc agcggttaca ggggacaaaa
     2701 cccaagccgc gtttcatctt ttgtcgtttc ttgaatacgc ctcagcacac cacagcgatg
     2761 cagcattcga tgcttctctt attttcacga gcgcccgctg ttctttagaa aaggcaatgc
     2821 tgcaaattga acgagaaacg aatgcgccgc tcgttgaatt aaatataggg agcgccgaca
     2881 cacactataa ccgcatcggc gctctcagaa atttgcaagc gcgcactttt tttgagagtg
     2941 catttttgtc gtccgagcgc acactgtggg caaacattat ggccacaacc gctgcggaca
     3001 atgaggccgt gtttcaactg cccgtgaaag ccacttggaa taaaaatccc gacgcgcaag
     3061 tggccgaaaa tccactttgg caccaccctg ctcttgcgaa ag
//
LOCUS       DAOMPD010000318         3099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12763_length_3099_cov_2.130092, whole genome shotgun sequence.
ACCESSION   DAOMPD010000318 DAOMPD010000000
VERSION     DAOMPD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12763_length_3099_cov_2.130092"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..546)
                     /locus_tag="PLY93_10520"
                     /pseudo
     CDS             complement(1..546)
                     /locus_tag="PLY93_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            782..1510
                     /locus_tag="PLY93_10525"
     CDS             782..1510
                     /locus_tag="PLY93_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRP69954.1"
                     /translation="MRKGEGALLPLVLFLIYVFDFDRTSFFIHALVGFVALALMYALN
                     DIHDAPFDLHDEVRKSAWVEALAQKQKVAYIIHALQLAFFIGVLIFYLKMTPLIVAAA
                     FTISTAYNLWGKKIRFLGDILVLFWGPTFTLLLSVKIPIFLVANTGFMLLMGHTFQMA
                     RDSHADAKNDIRTTWVVAPRWAVVIFYIICIAAQLCFAFGGFYAAAFAVTSFYLVPLF
                     GGYTQKSWLILKFLQASSWLLILV"
     gene            1648..2388
                     /locus_tag="PLY93_10530"
     CDS             1648..2388
                     /locus_tag="PLY93_10530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP69955.1"
                     /translation="MKTATTAPNLSIVLLCYKTEEHFVPYATQIIKAFNVVYSPFEMI
                     LVANYDAHDSTDKTPAMARCFASENKKCRVVARAKEGGMGWDLRSGLEIAQGEKIMFL
                     DGDGQTPVEDMVRLWNFSEETVADIWGIYRTPRRDGAIRKAMTLIFNTSIRILFPRLR
                     VRDINAKPKILTRRAYEIIQPKNSDWFIDAEVLLAAVNHHFTIRQIPGAFEANTWRKS
                     FVNLCVVFEFITNMIKARFHYWFTPKIK"
     gene            2385..>3099
                     /locus_tag="PLY93_10535"
     CDS             2385..>3099
                     /locus_tag="PLY93_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69956.1"
                     /translation="MKLPTYTDTYSSVKIPLVIALLCFIFYSGLVPYGKMRTVSDKFN
                     YHREVALAFLQGKLDITCPQKTGCHDLALYRGKYYIYQPPVPTLFMLPAIFIWGAATP
                     DALIAAFLGSLNVFLFGVFFITLRRKYENSTSPRERLRNNLRAIKSREEIIFILVWGL
                     GTAHYYISMLGDVWHITALCGQTFLLAALTAMLSEKVGWRIGAAIFFALAVFTRLNLV
                     TAAPFFAYLLWREKKSTSYF"
BASE COUNT          754 a          728 c          709 g          908 t
ORIGIN      
        1 ttcttttaag ttgtgcggtg gaatttttgt cgccataccg acggcgatgc ccgacgaacc
       61 gttaaccaaa agatttggcc acgccgctgg caatacttta ggctcgcggc gggtgccgtc
      121 gaagttatcg acgaaatcaa ccgtttcttt atcgatatct tttaaaagtt cttctgccat
      181 cgaggtgaga cgcgcttcgg tgtaacgata tgccgcaggg ggatcgccat cgacagagcc
      241 aaagtttcct tggccgtcga tcaagagttc gcgcatcgaa aaatcttgag ccatacgcac
      301 catcgtgtcg tacaccgctg cgtcgccgtg ggggtggtag ttaccaatca cttcccccac
      361 aatctttgcc gattttacat acgcccgatc ttgccgccac gcgcgttcgt tcatcgcatg
      421 gaggatgcga cggtgtaccg gtttgagtcc atcgcgcaca tcggggaggg cacgcccgac
      481 aatcacactc atcgcgtacg cgaggtacga ttttttcatt tggtcttcaa tttcgacggg
      541 gatgatttct tttgcaatgg tatcgggaac tgttgtgtgc tcactccctt ttccggtgcg
      601 cttaccttta gacatgcgac ataataaagg agagaaacac gtagtcgaga attaagtggt
      661 tggcgcgcaa gctcgaaaaa gtgcttttat cgaaaaaggc agtacgccgc gtatagcaca
      721 aacagtttag ccgagattgg tatacttaat tcttacgcct tttttctctc tttttacgcg
      781 tatgcgtaaa ggcgaaggcg cgcttttacc tttagtcctt tttttgatct acgtttttga
      841 ctttgatcgc acatcatttt ttatccatgc attggttggc tttgttgcgc tcgcgctgat
      901 gtacgcgctc aacgacatcc acgatgcacc ctttgatttg cacgatgagg tgcgaaaaag
      961 tgcgtgggtt gaagcgctcg ctcaaaaaca gaaagtagcg tatataatcc acgcactcca
     1021 gttggctttt tttattggag tgttgatttt ttatctaaaa atgactccac tcatcgttgc
     1081 tgcagcattt accataagca cggcatataa tttatgggga aagaaaatac gttttttagg
     1141 cgatatactt gtgctatttt ggggtccaac ctttacgctt ctcctgtcgg taaaaattcc
     1201 cattttctta gtcgctaaca ccggctttat gctccttatg ggacacacat ttcaaatggc
     1261 gcgcgatagc cacgccgatg caaaaaacga catcaggaca acgtgggtgg tggcgcctcg
     1321 gtgggcggtg gttatttttt acatcatttg tatcgcggca cagctttgtt ttgcatttgg
     1381 tggtttttat gctgcggcat tcgctgttac atctttttat ttggtgcccc ttttcggcgg
     1441 ctatactcaa aaaagttggc tcattttgaa gtttttacaa gcaagttcat ggcttctcat
     1501 tttggtgtag cgcacaggct ctgaggcaat gacgactaga ttattgttta caaaataggc
     1561 attgcttata aaaaatattc atgggcgcac tatacattct acgcgttctt tataacgcac
     1621 gccagtggcc gcacgctttt tgagattatg aagaccgcca ccacagcccc caatctctcc
     1681 attgtccttc tttgctataa aacagaagag cattttgtcc cttacgcaac acaaattata
     1741 aaagccttca acgtggtata ttcccctttt gaaatgattt tagtcgccaa ctacgacgca
     1801 cacgacagca ccgacaaaac gcccgctatg gcccgctgct ttgccagtga aaataaaaaa
     1861 tgccgtgtag tggcgcgagc caaagaaggc ggtatggggt gggatttacg cagcggcctt
     1921 gagatagcgc aaggagaaaa aattatgttc ctcgacggcg acgggcaaac accagtggaa
     1981 gacatggtgc gtctgtggaa ttttagcgaa gaaactgttg ccgacatttg gggcatctac
     2041 cgcacaccgc gccgagatgg cgctatccgt aaagcaatga cgcttatttt taacaccagt
     2101 atacggattc tttttccccg tttacgcgta cgcgacatca atgcaaagcc caaaattttg
     2161 acacgccgcg cttacgaaat cattcaacct aaaaatagcg attggtttat cgacgctgaa
     2221 gttttgctcg cggcagtaaa ccatcatttt acgattcggc aaatccccgg tgcatttgag
     2281 gcaaacacct ggcgcaagtc gttcgtgaat ttatgtgtag tattcgaatt tataaccaac
     2341 atgattaaag cgcggttcca ctactggttt actcccaaaa taaaatgaag ctacctacat
     2401 atacagatac ttacagcagc gttaaaatcc cccttgttat cgcattactc tgttttattt
     2461 tttactcggg tcttgtgcct tacggaaaga tgcgtacagt atctgataaa tttaactacc
     2521 accgtgaggt tgcgcttgcg tttcttcaag gcaaactcga cattacctgc ccacaaaaaa
     2581 ccggctgcca tgatttagcg ctctaccgtg gtaagtatta tatctaccag ccgccagtac
     2641 caacgctctt catgctgccg gccattttta tctggggcgc agcaacgccc gatgcgctca
     2701 ttgcagcttt tttaggtagc cttaatgttt ttcttttcgg cgtctttttt attacgttgc
     2761 gccgcaaata tgaaaacagt acaagtccac gcgagaggtt gcgtaacaat ctacgcgcca
     2821 taaaatcacg cgaagaaatt atttttatac ttgtgtgggg tttaggtacc gcgcattatt
     2881 atatatcgat gctgggtgac gtgtggcata tcacagcgct ttgtgggcaa acatttcttt
     2941 tggcggcgct tacggcaatg ctttcagaaa aggtgggatg gcgtattggg gcagcaattt
     3001 tttttgcgct cgctgttttt acgcgactca acctcgtgac ggcggcgccg ttttttgcgt
     3061 atcttctttg gcgtgagaaa aaaagtacat cttattttt
//
LOCUS       DAOMPD010000319         3071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12917_length_3071_cov_3.053714, whole genome shotgun sequence.
ACCESSION   DAOMPD010000319 DAOMPD010000000
VERSION     DAOMPD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12917_length_3071_cov_3.053714"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLY93_10540"
     CDS             <1..186
                     /locus_tag="PLY93_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69957.1"
                     /translation="VEGTESGRWITMDVKDINGDGLSEILLGAAYTPNFGAQGEGKQR
                     IPLGPSLLFMTPQKSKL"
     gene            complement(194..652)
                     /locus_tag="PLY93_10545"
     CDS             complement(194..652)
                     /locus_tag="PLY93_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69958.1"
                     /translation="MLKKNQTISLAIFSLILFTPYCKRKEAPHITELRQFFTSAANQF
                     EVESHHLDKTATPAEVKQSMLALEKVFSENIRAAKNLIQKYPQIMNERPTIQIILKEE
                     MHTLQKSIQLVARKVAYWQEKLAKDKEFNTIKKRIENLNNGFEFDTKSKD"
     gene            complement(653..2080)
                     /locus_tag="PLY93_10550"
     CDS             complement(653..2080)
                     /locus_tag="PLY93_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69959.1"
                     /translation="MRTLLAFGVFVGITRGALEQFLFGIAANGSDILAYIPFYISLPF
                     IYSALLSALPGIQYIKTLQPVTFASILGLLPPILDYIFSSTETHKVFYGYFLIHDLRE
                     FPWLGYNPQQNYSLGEAVTIWLTFIFASLYVYSRTRNFILAVCAFFLAYVAYILYALA
                     IPMGISFILFGYVPHRAPLEAMGPAPLRQGLFVISAVQVFIAWLIDTTFTGALKTYLK
                     RSLHILPFVLLVILGAIQGHATFFSTSFVFSCILLVGFTIIAQNDLRENNNIQNHIVP
                     TANALTIIFCVMLLFAGMRFSLIGFALIALAILYHYKFFNARKTLLGSMKIEGAWAFL
                     SYLMGLMAGKSAQPSTKIIITGALIFGGFSLFSLIKDAKDIDDDTKENRITLYTYVQK
                     QGWSTRTLHGFLVLALVLGLIIVALFLRENAVSFLIHTILSLVTIFAAVKAFHKIGFQ
                     LFLLALAAVIINLIVHEYLMLNRVQ"
     gene            complement(2133..2687)
                     /locus_tag="PLY93_10555"
     CDS             complement(2133..2687)
                     /locus_tag="PLY93_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69960.1"
                     /translation="MDCKRRSDNSAKTATDPQVQLITAFLSAPFMPDDKIDGLWIADK
                     KDLQSMLAAKFLKEYDARKDVVPLDEIKKNLEAAKYFLRVNNIECDELFLVVGGQATA
                     HPGEKKIQTGKVNTYDLTFSVMNTKTYEPFKVSATMSYDDKSDTLTMNQGGKPFRLYR
                     EKRSIEALVSEYAKPAVSVELPQY"
BASE COUNT          923 a          590 c          655 g          903 t
ORIGIN      
        1 gttgagggca cagagagcgg tcgttggatt acgatggatg taaaagatat aaacggcgat
       61 ggtttgtcag aaattttatt aggcgcggcg tatacaccta attttggggc tcaaggggaa
      121 ggcaagcaaa gaatacctct tgggccaagt ctattattta tgacgccaca aaaaagtaag
      181 ctatagacga ctgttagtct ttcgactttg tatcaaactc aaaaccgtta ttcaagtttt
      241 caatacgttt ttttatagtg ttaaactctt tgtctttggc gagtttttct tgccaatacg
      301 caactttacg cgcaactaac tgaatacttt tttgtagtgt gtgcatttct tctttgagga
      361 taatctgaat cgtcggtctt tcgttcataa tctgtggata tttttgaatt aagttttttg
      421 cggctcgaat gttttcacta aatacctttt ctaacgccaa catactttgt tttacttcag
      481 ccggtgttgc tgttttatct aaatggtgcg actcaacctc aaattggttt gccgccgatg
      541 taaagaactg cctgagttcg gtaatatgcg gcgcttcttt tctcttgcag tatggagtaa
      601 aaagtattag gctaaaaata gcaagcgaga tagtttggtt tttctttaac atctattgga
      661 cccgatttaa cattagatat tcgtgtacaa ttaaatttat gataacggca gcgagcgcaa
      721 gaagaaaaag ttgaaatcca attttatgaa aagcttttac cgctgcgaaa attgtcacaa
      781 gagaaagaat agtatgaatc aaaaagctga cggcgttttc tcgtaaaaag agagcaacga
      841 taattaaacc aagcactaag gcaagtacta agaacccgtg cagcgtgcgt gtactccacc
      901 cttgcttttg tacatacgta tatagggtta ttcgattttc ctttgtatcg tcatctatat
      961 cttttgcgtc tttaatgaga gaaaataaag aaaaaccgcc aaaaatgagt gcgccggtaa
     1021 tgataatctt tgtagatggt tgagcgcttt tacccgccat taaccccatg aggtaactta
     1081 aaaaagccca tgcaccctcg attttcattg aacctaaaag agttttgcgg gcgttaaaaa
     1141 atttataatg gtacaatata gctaaagcta taagcgcaaa ccctattaaa gaaaaacgca
     1201 tgcctgcaaa aagaagcata acacaaaaaa taatcgtgag tgcattcgcc gtgggtacta
     1261 tatgattttg tatgttgtta ttttcgcgta aatcgttttg agcgataatt gtgaagccta
     1321 ctaacaaaat gcatgaaaac acaaatgagg ttgaaaaaaa agtggcgtgg ccctgtattg
     1381 cccccaatat aactaagagt acgaatggta aaatatgtaa agagcgcttt aagtaagttt
     1441 taagcgcgcc agtgaatgta gtgtcgataa gccatgcgat aaatacctga actgccgaga
     1501 ttacgaacaa cccttgacgt aagggggctg gccccattgc ctctaaaggc gcacgatgtg
     1561 gtacatagcc gaagagtata aaactaatcc ccatggggat ggctaaagcg tatagaatat
     1621 aggctacgta cgcaagaaaa aatgcgcata cggctaaaat aaaatttcga gttctcgaat
     1681 acacatagag cgatgcaaaa ataaaggtca gccatatcgt cactgcttca ccgagtgaat
     1741 aattttgttg tggattatac ccaagccatg ggaattctct taaatcgtgg atgagaaaat
     1801 agccatagaa tactttatgc gtttcagtcg aactaaaaat ataatcaagt attggcggca
     1861 gtaagcctaa aatacttgca aaagtaaccg gttgtaatgt ttttatatat tggataccgg
     1921 gtagcgctga taaaagcgca ctatagataa atggtagcga aatataaaaa ggtatatagg
     1981 ccaaaatgtc gctaccgttt gcggcaatac cgaaaagaaa ttgttctaag gcgccgcgtg
     2041 ttatgccgac gaacacgcca aacgccaaga gcgtacgcat gtcaaacggg gtttttcttt
     2101 gccttgtaac ccaaaatcga tagaaggtca tttcaatact gcggtagttc tacagaaact
     2161 gccggtttgg catactcact gacgagcgct tctatgctgc gcttttcccg atagagccga
     2221 aacggtttac cgccttggtt catggtaaga gtatcgcttt tatcgtcata gctcatggtt
     2281 gcagaaactt tgaatggttc ataagttttt gtgttcatga cgctaaaggt taggtcgtag
     2341 gtattgactt tacccgtctg aattttcttt tcacctgggt gtgccgttgc ctggcctccc
     2401 accactaaaa agagctcgtc gcattcgatg ttattgacac gtaagaaata ttttgccgct
     2461 tctaagtttt tctttatttc atctaaaggc acaacgtctt ttcgagcatc gtattctttt
     2521 aagaacttag cggcgagcat cgattgtaaa tcttttttat cggcaatcca taaaccgtct
     2581 attttatcgt ctggcataaa gggagcagat aaaaatgcag tgataagctg cacttgtgga
     2641 tcggtggctg tttttgccga attatcgctt cttctcttac aatccattac caataagaga
     2701 agaaagcaga caaaagcatg ccttaaaaaa cgaggtatat ggtttgagcg acacacttgg
     2761 gttaatatac tatcgtcatt tttccattca ggttttaact atctcagtga gttggcgcac
     2821 aggctcctaa taggtgacat ctctcgtcga gagactcgaa gaggagattt gaaaactcgt
     2881 cacggcaacc gctttttaaa aacaactcct gttatggagg ccccacgcgt tgactaggcc
     2941 caagcgatgg atccacttta caagacgtgg tttgtgagta atctttaatg gctttccagg
     3001 taccgccatt agagatacac cgttgtgcgt cagtgctacc gctacctgtt gttgttgaag
     3061 cgcctgagca a
//
LOCUS       DAOMPD010000320         3068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12935_length_3068_cov_2.135081, whole genome shotgun sequence.
ACCESSION   DAOMPD010000320 DAOMPD010000000
VERSION     DAOMPD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_3068_cov_2.135081"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLY93_10560"
     CDS             complement(<1..212)
                     /locus_tag="PLY93_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69961.1"
                     /translation="MIQQPFISKRSEGVYELSETGVERAYLLTMQRLLLHYAQQALKI
                     PPAEAQKLGQQLPALVQKIIKKLDVY"
     gene            complement(216..647)
                     /gene="aroQ"
                     /locus_tag="PLY93_10565"
     CDS             complement(216..647)
                     /gene="aroQ"
                     /locus_tag="PLY93_10565"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRP69962.1"
                     /translation="MQITVVNGPNLNLLGTREAAHYGTFTLADLEKKLRTHAGIKATL
                     TFFQSNHEGVLIDFIQKLTVKDRLILNAGAFTHTSVAIRDALTATQVPFIEVHISNVH
                     AREAFRHHSHLSPIAQGVIVGLGIESYLLAVDYFLRMHTTL"
     gene            complement(655..1752)
                     /gene="aroB"
                     /locus_tag="PLY93_10570"
     CDS             complement(655..1752)
                     /gene="aroB"
                     /locus_tag="PLY93_10570"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRP69963.1"
                     /translation="MVKKNNAIAVKLGTRSYAIEYAQNLTTLSASLPKVQGKEFALID
                     ANVEKYHGKALKAALPQITFLRIPHGERSKSFRALENLANALVREGATRHSRLYAIGG
                     GVVGDLTGFLAGVFMRGISFVQIPTTLLAMVDSSVGGKTAVNIAAGKNLVGVIHQPQH
                     VFIHMPFLKTLPQRELRCGLSESIKTALIYDKKLIKYLEKFEYNPKKIPTEFLAHLSL
                     ECIRTKATIVSKDEKEQNIRAFLNFGHTLAHAIEAKLQYKKVLHGEAVAIGQRFAALL
                     SRRLGFLTKEDELRIENLLHKFNLPTRLKDAFPNGVNPTLLLNLMYADKKNRGREIRF
                     VLLSALGKAQLPQKVEEQELLASLKEFQMLA"
     gene            complement(1745..2905)
                     /locus_tag="PLY93_10575"
     CDS             complement(1745..2905)
                     /locus_tag="PLY93_10575"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRP69964.1"
                     /translation="MQNYARMPVAFQYGAGDRLYDTEGKEYIDFLSGIAVTALGHAHT
                     DLIAALTNQAEMLWHTSNLFYNQQQAQLARALVEINFPGKVFFCNSGAEANEAAIKLM
                     RGYGQKKKPQRQKIVALKDGFHGRTFGAMSVTGQDKIQKGFGEILPHIEFVAPDDIAG
                     LASAIDSNTCGVIMEPVIGEAGVMPLSLDFLKIARARCNEEEALLCFDEIQIGMGRSG
                     HYFACQHYGVLPDLVTMAKGLGGGFPMGALLVAEKYADVFAHGMHGSTFGGNHLACAV
                     AYEVIRTMESEKLLEHVRSASRYLTAALKKLKEKYPTKIIEVRGLGLLIGIVLKEGIE
                     TGPLLSRALELGLLIGRAGASVLRLAPPLNVRKATMDAALQKIDALIGELIG"
BASE COUNT          721 a          714 c          790 g          843 t
ORIGIN      
        1 aaatagacgt cgagtttttt aataattttt tgaacgagtg cggggagttg ttgccccaat
       61 ttttgcgctt cggcaggggg aattttcaac gcctgctgcg cgtagtgcaa caacaagcgt
      121 tgcattgtga gcaagtacgc gcgttccacc cccgtctcgg agagttcgta aacgccttcg
      181 cttcgtttgc tgatgaaagg ttgctggatc atcgcttaaa gagtggtgtg catccttaaa
      241 aaataatcga cggcgaggag gtaactctcg atacccaaac ccacaatcac tccctgtgca
      301 atcggcgaca ggtgtgagtg gtggcggaat gcctcgcgcg cgtgcacgtt tgaaatatgc
      361 acttcaataa aaggaacttg cgtcgccgtc agcgcgtcgc ggattgccac cgaggtgtgt
      421 gtgaacgcgc ccgcattcaa aatgagtcta tcttttacgg tgagcttttg tataaagtcg
      481 ataagtacgc cttcgtggtt cgattgaaaa aaagtgagcg tcgctttgat accagcgtgt
      541 gtccgcaatt ttttttctaa atcggcgaga gtgaatgtgc cataatgcgc tgcctcgcgc
      601 gtaccgagta aatttaaatt ggggccattg actactgtaa tttgcatgag acgtttatgc
      661 taacatttga aattctttca ggctcgcaag tagttcttgc tcttcgacct tctgcggtaa
      721 ttgggctttc cccaaagcag aaagtaaaac gaaacgaatt tctcttcctc tatttttttt
      781 atcggcgtac atgaggttta agagtagagt aggatttact ccgttgggaa atgcgtcttt
      841 gagtctggtg ggtagattaa acttgtgtaa aagattctcg atacgcagtt catcctcttt
      901 cgtgagaaat cccaaacgac gtgaaaggag cgcagcaaaa cgttgaccga ttgccaccgc
      961 ctcgccgtgt agaacttttt tatactgaag cttcgcttca atcgcgtgcg cgagggtatg
     1021 tccaaaattt aagaacgcac gaatgttttg ttctttttcg tctttcgata caattgtggc
     1081 tttggtgcgg atacactcca acgaaaggtg tgcgagaaat tcggttggga tttttttagg
     1141 attgtattca aatttttcga gatacttgat taatttttta tcgtagatga gcgctgtttt
     1201 gatggattcg gataatccgc accgcagctc gcgttgaggt agagttttta agaaaggcat
     1261 gtggataaaa acgtgctgcg gttgatggat cacaccgact aaatttttac cggcggcgat
     1321 attgactgcg gttttgcctc ccactgacga atcaaccatc gccaaaagcg tggtggggat
     1381 ttgtacgaaa gaaatcccac gcataaaaac gccggctaaa aaaccggtca ggtctcccac
     1441 cacaccgcca ccgatggcgt aaaggcgcga gtgacgggtt gcaccttctc gcacaagagc
     1501 gttcgcaagg ttttcgagcg ctctaaagct tttgcttctt tccccgtgag gaatgcgcag
     1561 aaaagtaatc tgtggaagcg ccgctttgag agccttgccg tggtattttt cgacattggc
     1621 gtcgatgagc gcaaattctt tcccttgtac tttagggagc gaagcgctga gcgttgttaa
     1681 gttttgagcg tactcaatgg cgtacgaacg cgtgcccaat ttaacagcga tggcgttatt
     1741 ttttttaacc aatcagttct cctataagag cgtcgatttt ttgaagtgca gcgtccatcg
     1801 tcgctttgcg cacattgagg gggggagcca agcgcagcac agaagcgccg gcgcgcccga
     1861 tgagtaatcc taactcaaga gcgcggctaa ggagtggtcc agtttcgatg ccttctttaa
     1921 ggacaatacc gataaggagt cccaaaccgc gcacctcgat aattttagtc gggtattttt
     1981 ctttgagttt tttgagcgct gccgtcaaat agcgcgatgc gctacggaca tgttcgagta
     2041 atttttccga ctccatcgtg cggatgactt cgtacgccac cgcgcacgcg agatggttac
     2101 caccaaaggt cgaaccgtgc atcccatgcg caaatacatc ggcgtatttt tcggcaacca
     2161 aaagcgcccc cattgggaaa cctcccccca atccttttgc catggtgaca aggtcgggta
     2221 gcacgccgta atgttggcag gcaaagtagt ggccgctacg tcccatcccg atttggattt
     2281 cgtcaaaaca gagcagggct tcttcttcgt tacaacgtgc gcgggcaatt ttcaaaaaat
     2341 ctaatgacaa cggcatcact cctgcctcgc caatcacggg ctccataatg acaccgcaag
     2401 tgttggagtc gatggcggaa gccaagccag cgatgtcgtc gggagcaaca aattcaatat
     2461 gcggtagaat ttcaccaaac cctttctgaa ttttatcttg gccagttacc gacattgcgc
     2521 cgaaggtgcg cccatggaaa ccgtctttga gagcgacgat tttttgccgt tgtggttttt
     2581 tcttttgtcc gtacccacgc atgagtttta tcgccgcttc gtttgcctcg gcgcccgaat
     2641 tacagaaaaa gacttttccg ggaaaattaa tttcaacgag cgcgcgtgca agttgcgcct
     2701 gttgttggtt gtaaaataaa ttcgaggtgt gccaaagcat ttccgcttgg ttggtgagcg
     2761 cggcgatgag gtcggtgtgc gcatgcccca acgcggtgac ggcgatgccg cttaaaaaat
     2821 cgatatactc tttgccctcg gtatcgtaaa ggcggtcgcc tgcgccgtat tgaaatgcga
     2881 ccggcatgcg tgcgtagttc tgcaaaagat attttttatc gagctcttgg atttctttga
     2941 gcgaaagtgt agcttaaggg tcgaacgcgc cgtggttgtg agatgcctct cgatgcgcgt
     3001 gtgtgtcgtc gccgatgccg taaagctcac gcgcgcttgt tgtcggttta atttttccgc
     3061 ccatgata
//
LOCUS       DAOMPD010000321         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_12970_length_3061_cov_3.076181, whole genome shotgun sequence.
ACCESSION   DAOMPD010000321 DAOMPD010000000
VERSION     DAOMPD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12970_length_3061_cov_3.076181"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLY93_10580"
     CDS             complement(<1..751)
                     /locus_tag="PLY93_10580"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRP69965.1"
                     /translation="MARTEFYFDKNPEPHLGRTAAILKAHPEMRKFIGKKNPWSAALI
                     LFTVALQLTIAYFLREQEWYIILPAAWLVGTLINHAYIGLLHDASHALIFRGRFWNDV
                     FGLIVNAPMLVPSYVSFKKYHMKHHAFQGVYELDADLPAHWEVKTIKNVWYRKLLWLA
                     FYPLIQTLRTMRVREVAFFDGWVLANWITTFATDFAVYYFMGPMAFLYVALSFWFGFG
                     LSIVGGRLIQEHYMVAEPQETYSYYGWLSLPS"
     gene            916..2070
                     /locus_tag="PLY93_10585"
     CDS             916..2070
                     /locus_tag="PLY93_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP69966.1"
                     /translation="MVADALAKKLRVAWVADIVDEVSGVITDTEEMYDLAQSHGYFWQ
                     PVTTYHKPIKPFYIFDPILPIPIGRFYAGTTFYVPDFLRVVQFFRKNRVNVIVSNTPG
                     SMGMLAMAAAKYLSIPWVDIYHTDIDYYVNDLTGRITKPLLKNPALFFLKQYQKQADL
                     IFVRTEEFYDLMIKKGHPKKKLRYYPAGVNARHWNPKNIDRKIFSQFGIDPTRTIVIF
                     VGRITKVKDIEFLLRYFVEQKPENAVCAVIGGGPEKELYEKKYEGENVKFLGVQRGAA
                     LQKLYASADLYVLPSASETLGKTVLEAMASGTTVVVSDKGGPKDYVTHNENGVVFKAH
                     SYASFKQAMNGVLADRARLETLGINAREAMANHTDEKLFEAFTGHIAELL"
     gene            complement(2076..>3061)
                     /locus_tag="PLY93_10590"
     CDS             complement(2076..>3061)
                     /locus_tag="PLY93_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP69967.1"
                     /translation="GREVLHAQDEMLPFLRLDRVLGYPPVEEETIEHRHAILVDLHGE
                     KFALGMDEFLKRRDLVVKSLNQNYKHVPGLAGASMLGDNEIVFILDLAEVIELHRSKE
                     KPARSETMNLNKSETLNSNTPKEPVEGMVANNTAVRIEKLFDPADKEVFRNWISQSNK
                     AAIKGIQMLTGSQAITVKKSKGARVKAGNSRQIMERIVQNADNIYLLHLPMIPDTGSI
                     DLILKRDSAERMARLLFSAAGLDFEGEFDPSPLLEITNILGSAYTNTLTFLTETSVEP
                     ATPSLLETKEEIHALIEGRLKAPHADILVVDNEFHIKNENIAVELVIYLNS"
BASE COUNT          808 a          717 c          759 g          777 t
ORIGIN      
        1 aagatggcag cgacagccaa ccgtaatagc tatacgtctc ctgcggctcg gcgaccatgt
       61 agtgttcttg gatgaggcga ccaccgacga tggaaagacc aaagccaaac caaaacgaga
      121 gggcgacata gaggaacgcc attggtccca taaaataata caccgcaaaa tcggtagcaa
      181 aagtggtgat ccagttagcg agtacccaac cgtcgaagaa cgccacttcg cgcacgcgca
      241 tagtgcgcaa tgtttgaatt aaaggataaa atgctaacca caacaatttg cggtaccaaa
      301 cgttttttat ggttttgact tcccaatgcg cgggcaaatc ggcatcgagt tcgtacacgc
      361 cttggaatgc gtggtgcttc atgtgatatt ttttgaacga cacatacgac ggcacgagca
      421 ttggtgcatt gacaatgaga ccaaaaacat cgttccaaaa ccgcccacga aaaataagcg
      481 cgtgcgaagc atcgtgcaaa aggccgatgt acgcgtgatt gataagggta ccgacgagcc
      541 atgcagcggg taaaataatg taccactcct gttcgcgtaa aaaataagca atcgtgagct
      601 gcaacgcaac agtgaaaaga atgagcgcag cgctccatgg attttttttg ccgatgaact
      661 tgcgcatctc ggggtgcgct ttcaaaattg ccgcagtgcg gcctaagtgc ggttcgggat
      721 ttttatcaaa ataaaactca gtccgtgcca tacgtctttt tgtgcgttgc aaacaggcgg
      781 taaagtagaa tgaaataagg agcttgtgcg ccaactcgcg cagcgtacaa aaaactgcaa
      841 aaaaattgtc tccgtgagtt ggcgcacagc tctccggtat ctacttgacg ttgtagcgcg
      901 catcgtctac ctgaaatggt ggctgatgca ttagcaaaga agttgcgtgt ggcgtgggtg
      961 gccgacatcg tcgacgaagt ttccggagta attaccgaca ccgaagaaat gtacgattta
     1021 gcgcaatcgc acggttattt ttggcaaccg gtgacgacgt accacaaacc gattaaaccg
     1081 ttttatatct tcgatcccat tttacctata cccattggac ggttttatgc gggtacaaca
     1141 ttttatgtac ccgatttttt acgtgtggtg caatttttca gaaaaaatcg agtcaacgtc
     1201 atcgtctcga acacacccgg ctcgatgggt atgctcgcta tggccgcagc aaaatatctc
     1261 tcgattccat gggtggatat ttatcacacc gacatcgact attacgtgaa cgatctcacc
     1321 ggtcgcatca ccaaaccgct gctcaaaaat ccagcgctct tttttttgaa gcaataccag
     1381 aagcaggccg atcttatttt tgtacgtacc gaagagttct atgacttaat gataaaaaag
     1441 ggtcacccga agaaaaaatt acgctactac cctgcggggg tgaatgcacg gcattggaac
     1501 cccaaaaaca tagatagaaa aattttctcc caattcggta ttgatcccac tagaacgatt
     1561 gtcatttttg tcggacggat cactaaagtc aaagatattg aatttttgtt gcgctatttt
     1621 gtcgaacaaa aaccagagaa tgcggtgtgc gccgtgattg gcggtggtcc agaaaaagaa
     1681 ctttatgaaa aaaaatacga gggcgagaac gtaaaatttt taggcgtgca gcgcggcgcc
     1741 gcattgcaga aactttacgc aagcgccgat ctttacgtgt tgccgtcggc gagcgagaca
     1801 ttggggaaaa ctgttctcga ggcgatggcg tccggcacca ctgttgtggt ttcagataaa
     1861 ggcggcccga aagattacgt gacgcataat gaaaacggtg tggtgttcaa agcgcacagc
     1921 tacgcgtcat ttaagcaagc gatgaatggc gtactcgccg accgtgcgcg ccttgaaacc
     1981 ttgggtataa acgcacggga agcgatggcg aaccacaccg acgaaaaact tttcgaggca
     2041 ttcaccggcc acatcgccga gctgttgtaa accgatcagc tgttcaagta aatcactaat
     2101 tcgacggcaa tattttcgtt tttaatgtgg aactcgttgt cgacgacgag aatatcggcg
     2161 tgcggcgctt ttaaccgtcc ttcaatgagc gcgtggattt cttctttggt ttctaacaac
     2221 gatggtgtcg caggttcgac gctggtttcc gttaagaagg tcaacgtgtt agtgtatgcg
     2281 ctccctaaaa tattagtgat ttctaaaagc gggctagggt cgaactcgcc ttcaaagtcg
     2341 agccccgccg cgctaaacag gagacgcgcc atgcgttcag cgctgtctct ttttaagatg
     2401 aggtcgatcg accctgtgtc gggaatcatg ggcagatgca gcaggtaaat attgtccgcg
     2461 ttctgtacga tacgctccat gatttgccgc gagttgcctg ctttaacacg tgcgccttta
     2521 cttttcttta cggtgatcgc ttggctgccg gtgagcattt ggatgccttt gatggcagct
     2581 ttgttgcttt gggaaatcca gttacgaaac acctctttat cggcagggtc gaaaagtttt
     2641 tcaatgcgca ctgccgtgtt gttcgccacc attccttcaa cgggctcttt gggtgtgttc
     2701 gagttcaggg tttcgctttt gtttaagttc atagtttcgc tccgcgcggg tttctctttg
     2761 ctgcggtgta attcaatcac ctctgcaagg tctaatataa acacaatttc attgtcgccc
     2821 aacatggaag ctccggcaag accgggtaca tgtttatagt tttggttgag cgacttgacg
     2881 accaagtcgc gccgcttgag aaattcatcc atacccaaag caaatttttc accgtgcaaa
     2941 tcgacgagaa tggcgtgacg gtgttcgatg gtctcttctt caacaggggg ataacctaac
     3001 actcggtcga gccgcaagaa gggcagcatt tcatcttgcg cgtgcaaaac ttcgcgccct
     3061 t
//
LOCUS       DAOMPD010000322         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13027_length_3049_cov_3.239479, whole genome shotgun sequence.
ACCESSION   DAOMPD010000322 DAOMPD010000000
VERSION     DAOMPD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13027_length_3049_cov_3.239479"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2014
                     /locus_tag="PLY93_10595"
     CDS             <1..2014
                     /locus_tag="PLY93_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRP69968.1"
                     /translation="QYRWFLFSLGYSAHNTISIHDGFRMGVGAAEHFTFGDAFLFYNL
                     NIAKENNTATHGIVASVRLGGIDSDPPEITFTPDGNSFSPNDDGVRDLLIFNATVSDK
                     SPVVYYELRIHDANGKMVYQLKSDERLREKSFSFALFFRSFLAPRARADIPEQFTWNG
                     RVFSDASKKVKDTVFEDEPQVEKLPDGTYHYEFWVLDEKNNESKHATGAIIIDTKKPI
                     ASVELDSDLISPNGDGQRDILTITQEVTTDDSYEAFVLATNGEKVRRYAWKNNAPTRV
                     EFDGKKDNGDLLAEGVYRYQLVGYNAAGNRNEAMSGNFYVSRRVDTVFLKSSNVGLNP
                     LQKSFAEVVLSPSVTYSEGYKEGAIFIRKDCESKKEEDILFQLPVTTLEESATAKNKK
                     SKKNKSALYVWRGESKDGKRLPDGVYCLSFRAHYENGNTPVSPPVQIVLDTTGPELDV
                     VADLEVRQFSPDGDGENEEQVFRLSSDDLTGIDSYALTIEEVVITDKETKYLPVRRFE
                     GKGIMPRSIYWDGRTESGALVESLTQYQYTLSAVDNYGNTSTTQGRRFETGVLVTPRV
                     NGFFIRLPQVQLDNLTGDTARLLLSTLNKYAKYKIKLEVHTTPSRGIETGLRRTEKAA
                     RALYGYFVDKGIAPDRLSYQGFGDTAPVYRGAVAVKNNRVDVYLSR"
     gene            complement(2025..2630)
                     /locus_tag="PLY93_10600"
     CDS             complement(2025..2630)
                     /locus_tag="PLY93_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRP69969.1"
                     /translation="MSSLHKLEPEGGWYKTDRPRIEPISEKKANILMKAVLWGASQVS
                     KNQTGTKKIPDVFLLLMQHQHIFWQWLQFASRLMPFGTLERRETELMILRVAWNTRCY
                     YEWGQHISLSKREGILVEDIERVAKGPNAEGWSPRVRAMLTATDELCLNGIVSSATWT
                     NLTEHLDAGKMIELLLLVGAYQMLAGVLNSVGLPLEEEFSG"
     gene            complement(2627..>3049)
                     /locus_tag="PLY93_10605"
     CDS             complement(2627..>3049)
                     /locus_tag="PLY93_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP69970.1"
                     /translation="LFGLPWHAAYSASKFGLRGVSEVLRFDLARHNIGVSLVCPGGVD
                     SGLVDTIKIAGIDTSDAEVAALREQFQKRAVPPESVAKDILRGIHRNKYMVFTSLDVQ
                     IGHFFQNTFALPYEAVMHFLNDKIQDIADNQKKKKDNL"
BASE COUNT          803 a          747 c          741 g          758 t
ORIGIN      
        1 gcaataccgc tggtttttat tctctctcgg ttattccgcg cacaacacca tctccatcca
       61 cgacggcttt cgcatgggcg tcggagctgc cgaacatttt acttttggcg atgcgttttt
      121 attttacaac ctcaatatag ccaaagagaa caacaccgcc acgcatggta tcgtcgccag
      181 cgtgcgtttg gggggtatcg atagcgatcc gcctgaaatt acatttaccc cagatggaaa
      241 ctccttttct cccaacgacg acggagtgcg cgatttgttg atttttaatg cgacggtgag
      301 cgacaaaagc cctgttgttt attatgagct gcgcatacac gatgcgaacg gtaaaatggt
      361 ttatcaactt aaaagcgacg agcgtttgcg cgaaaagagt tttagcttcg cacttttttt
      421 ccgtagtttt cttgccccgc gtgcgcgtgc cgatataccc gaacaattca cttggaacgg
      481 acgcgtcttt tcagatgcga gtaaaaaagt gaaagacacc gtctttgaag acgagccaca
      541 agtggagaag cttcccgacg gtacatacca ctatgaattt tgggtgctcg atgaaaaaaa
      601 taacgagagc aagcatgcta ctggcgcaat tatcatcgac actaaaaagc ccatagcaag
      661 cgtcgaactc gacagcgatt taatttcccc caatggcgac ggccaacgcg acatactcac
      721 aatcacccaa gaggttacaa ccgacgacag ttacgaggct ttcgttctgg cgaccaacgg
      781 tgaaaaggtg cgtcgttacg cgtggaaaaa caatgcaccc actcgtgttg agtttgatgg
      841 taaaaaggac aacggtgatt tactcgctga aggcgtgtat cgctatcaac tcgtcggcta
      901 caacgctgcc ggcaaccgca acgaagcgat gagcggcaat ttttatgtgt cgcgccgcgt
      961 cgacaccgtg tttctcaaaa gctccaatgt gggattaaac ccgttacaaa aatcttttgc
     1021 cgaagttgta ttatccccca gcgtcactta tagcgaaggc tacaaagaag gcgctatttt
     1081 cattcgaaaa gattgtgaaa gcaagaaaga agaagatatt ctatttcagc tcccggtcac
     1141 tacgcttgag gagagcgcta cggcaaaaaa taaaaaatca aagaaaaaca aatctgcttt
     1201 atatgtatgg cgtggagaaa gcaaagacgg taaacgccta cccgatggtg tgtattgcct
     1261 ttcgttccgc gcgcattatg aaaatggcaa caccccagta tcccctcctg tgcaaattgt
     1321 tttagataca accggacctg aactcgatgt agtcgccgat cttgaagtgc gtcaattttc
     1381 acccgatggc gatggagaaa acgaagagca agtgtttcgg ttatcgagcg acgatctaac
     1441 cggtattgat tcgtatgccc tcacgattga agaagtggtt atcaccgaca aagaaactaa
     1501 gtatttaccc gtgcgccgct ttgaaggaaa agggataatg ccgcgttcca tttattggga
     1561 tggccgcaca gaaagcggcg ctcttgtcga atcgctcact caataccagt acacattgag
     1621 tgcggtggat aactatggca acacgagcac gacacaggga cgtcggtttg aaacgggcgt
     1681 tctggtcacg ccgcgggtga acggcttttt tattcggttg ccgcaagtgc agctcgacaa
     1741 tttaaccggc gacaccgccc gtctactttt gagtacgctc aacaagtacg cgaaatataa
     1801 aatcaaactc gaagtgcaca caacaccttc gcgcggcatt gaaacgggat tgaggcgtac
     1861 tgaaaaagcg gcgcgtgcgc tatacggata ctttgtcgac aaaggcattg cgccagatcg
     1921 cttaagttat caaggttttg gcgacactgc gcctgtttac cgtggagccg tggcggtaaa
     1981 aaataatcgg gtcgatgtct acttgagtcg atgactttgc aagcctatcc gctaaactct
     2041 tcttctaaag gaagccctac gctgttcaaa acccctgcga gcatttggta tgcacccacg
     2101 agaagcaaga gctcgatcat tttgcctgcg tctaagtgct ctgtgaggtt tgtccatgtt
     2161 gcgcttgaaa cgatgccatt caggcaaagc tcgtcggttg cagtgagcat cgcacgcaca
     2221 cgcggcgacc aaccttcggc gtttgggcct ttggcgacac gttcgatgtc ttcgactaaa
     2281 attccttcgc gtttagaaag agaaatatgc tgaccccact cgtaatagca acgagtgttc
     2341 cacgcaacgc gcaaaatcat gagttcggtt tcgcgacgtt ccaacgtgcc aaacggcatg
     2401 aggcgcgaag caaattgtaa ccactgccaa aaaatatgct ggtgttgcat caggagtaaa
     2461 aagacatcgg ggattttttt cgtaccggtt tgatttttac ttacttgcga tgcaccccac
     2521 agcactgctt tcatgaggat gttcgccttt ttttctgaaa tgggttcgat gcggggacga
     2581 tctgtcttat accaaccgcc ttcgggttca agtttgtgga gtgaactcat agattatcct
     2641 tcttcttttt ttgattgtcg gcgatgtcct ggattttatc gttcaaaaag tgcattaccg
     2701 cttcgtaagg gagcgcaaac gtgttttgga agaaatgccc aatttgcacg tcgagcgaag
     2761 taaacaccat gtatttgttt cgatggatgc cgcgcaaaat atcttttgct acactttctg
     2821 gaggtaccgc gcgtttttgg aattgttcgc ggagggcggc aacttccgca tcgctcgtgt
     2881 cgatgcctgc aattttaatc gtgtcgacaa ggccggagtc cacgccacct ggacacacaa
     2941 ggctcacgcc gatgttgtgc cgcgctaaat cgaaacgcaa cacttccgaa acgccgcgca
     3001 aaccgaattt actcgcgcta tacgccgcgt gccacggcaa gccaaagag
//
LOCUS       DAOMPD010000323         3042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13069_length_3042_cov_2.175762, whole genome shotgun sequence.
ACCESSION   DAOMPD010000323 DAOMPD010000000
VERSION     DAOMPD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13069_length_3042_cov_2.175762"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PLY93_10610"
     CDS             <1..1381
                     /locus_tag="PLY93_10610"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP69971.1"
                     /translation="VWSQSETPPPYLMGGSLVQGYTFNPNHPTDGYNGTVTWTDRANE
                     YQLNQAWTYAEIPTDTSKKEFDWGGRIDLLFGSSYRWTTSSGFEDKIFKTNTGINSNC
                     TALPATGTQGTAKDNSNCNTVSSQYGLSIPQLYIDAAYKSFKLRIGHIISPVGFFTVD
                     TTQNFFAVLPYTYQYGEPFTHWGTWVYWAVNERLNAMGGATYGGDNFDGSGTGSKLPG
                     FLGTVNYIFADKSTLDWVVHISSEYNYKSAANSTDPAILSGDFKSGSYNRFESLYSWR
                     YFQTLVYKRNLTTKLQWVLQSDFGIQKVADFEAALTGSHTLDKEVYAMWYGINTYFFY
                     ALTDVLRVGLNLEWFRDQNGVRVGSVLPSMAATSTRAAGMGTGSSPFRGNYIGNFFAI
                     TIGPRFQVQKNTFVRFMARYDYFDDAALNALKPGDTGYNQTGSAALPFGDGNSRNQLI
                     FGIDLGVVF"
     gene            1414..2748
                     /locus_tag="PLY93_10615"
     CDS             1414..2748
                     /locus_tag="PLY93_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRP69972.1"
                     /translation="MKKNSISFAISSLMLSVCLFAGMTPAFAEANDVAAKIDSGRTAW
                     MLVSSALVLLMIPGLALFYAGMVRQKNMLNTILMSLIAMSVVGVEWILVGYNMVFGKS
                     IGGIFGWSEGGFALQNIPWDTTTNGIPTLVNVMFFGKFAIITPALISGAIVERMKFSA
                     YVLFILLWSVLIYNPLAHMVWGPGGWLLEKGVLDFAGGTVVHISSGFSALFLAWLFLK
                     KRVGYPEDQMRPGSLFQTLLGAGLLWVGWFGFNAGSAIATSADFMLRAGLAFTNTQIA
                     AAAAGFVWITIEYALHKKPTSVGLASGMVAGLVGITPGAGHVSPMAALAICSLAALVC
                     FTAVVAKRRLGYDDSLDVFGVHGMGGLFGALATGLFASVGTPHIGLFSGGDAQQFLLQ
                     LLGAGVGIAVAGVGTIVIAFIVDKTVGLRVSVKQETLGLDLSQHGETSLTRV"
     gene            2772..>3042
                     /locus_tag="PLY93_10620"
     CDS             2772..>3042
                     /locus_tag="PLY93_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRP69973.1"
                     /translation="MKLIIALIQPHRLEAVKRELNKREIYRLTVIEAMGYGRQKGQLQ
                     YFRGQEMESELINKIELQIGVNDAFVEPTIEAIMAGARSDSVGQIG"
BASE COUNT          730 a          763 c          785 g          764 t
ORIGIN      
        1 tgtctggagc caaagtgaaa ctccccctcc gtacctcatg ggcggcagtc ttgtccaagg
       61 ctacaccttc aatcccaacc accccaccga cggttacaac ggtactgtaa cttggacgga
      121 tagagccaac gagtaccaac tgaaccaagc gtggacatac gccgaaatcc ccaccgacac
      181 cagcaaaaaa gaattcgatt ggggtgggcg tatcgacctg ttgtttgggt cgagttaccg
      241 gtggacaacg agtagcggct ttgaagataa aattttcaaa accaacaccg gaataaacag
      301 taattgcacc gctctgcccg caacaggaac gcaaggcact gcaaaagata actcaaactg
      361 taacacggtg agtagccaat acgggctatc gataccgcag ctttatatag atgcggcata
      421 caagtcgttc aaactgcgta tcggacacat catttcaccc gtgggatttt tcaccgtcga
      481 caccactcaa aacttttttg cggtgctgcc gtacacgtac caatacggtg agcctttcac
      541 gcattggggt acttgggtct actgggcggt gaacgaacgc ctcaatgcca tgggtggtgc
      601 tacgtatggc ggcgataatt ttgacggctc gggtacgggc tcaaaactcc ccggattttt
      661 aggcactgtc aattatatct ttgcagataa atcgacgctc gactgggtcg ttcatatcag
      721 cagtgaatac aactacaaat cggcggccaa tagcacagac cctgcaattc tttcggggga
      781 tttcaaaagc ggatcgtaca accgcttcga gagcctctat agctggcgtt acttccaaac
      841 gttagtctat aagcgtaatt taacgacaaa attacagtgg gttcttcaat ccgattttgg
      901 catccaaaaa gtggccgact tcgaagcagc gttgactgga tcgcacacac tcgacaaaga
      961 agtgtatgca atgtggtatg gtatcaacac ctacttcttc tatgcactca ccgatgtatt
     1021 gcgcgtggga ctcaatcttg aatggtttcg tgatcaaaac ggcgtacgcg tcggcagtgt
     1081 tctaccaagc atggcggcaa cgtcaacacg cgctgcgggc atgggtacgg ggagctcacc
     1141 tttccgcggg aactacatcg gtaatttttt tgccatcacc attggtcccc gcttccaagt
     1201 acagaaaaat acgttcgtgc gttttatggc acggtacgac tatttcgacg acgctgcact
     1261 caatgcatta aaaccaggag atactggcta taaccaaaca ggcagcgcag cgcttccctt
     1321 tggcgacggc aatagccgca accaactaat ctttggcatc gacctgggag tggtgttcta
     1381 atgcgtacag caaataacaa aaggaggatt actatgaaaa agaattctat atcgttcgct
     1441 atctcttcgt taatgctatc ggtttgtctc tttgccggaa tgacaccggc gttcgccgaa
     1501 gcaaacgatg tggcggcaaa aattgactcc ggtagaaccg cgtggatgct cgtatcctcc
     1561 gcgctggttc tcctcatgat acccgggctt gcgctttttt atgcaggcat ggtgcggcaa
     1621 aaaaatatgc tcaacacaat cctcatgagc ctcatcgcca tgagtgtcgt tggtgttgag
     1681 tggattcttg tcggttacaa catggtgttt ggtaaatcca tcggcggcat ctttggatgg
     1741 tctgagggcg gctttgccct acagaacatc ccatgggata ccaccacaaa cgggataccg
     1801 acattggtga atgtgatgtt cttcggcaag ttcgcgatta tcacccccgc gcttatttca
     1861 ggcgcaattg tcgagcgcat gaagtttagc gcgtacgtgc ttttcatttt gctttggagt
     1921 gtactcatct acaaccctct cgcgcacatg gtgtgggggc caggcggttg gctcttggaa
     1981 aaaggagttt tagattttgc cggcggcacg gtggtgcata ttagctctgg attttctgcg
     2041 ctatttctcg catggctttt ccttaaaaag cgcgtcggtt atcccgaaga ccaaatgcgc
     2101 cccggaagcc ttttccaaac actattaggc gctggcctgc tttgggtagg ctggttcggt
     2161 tttaacgccg gttctgccat cgccacatcg gcggatttta tgttgcgtgc agggttggcg
     2221 ttcaccaata cacagattgc tgcggccgcc gccggttttg tgtggatcac cattgaatac
     2281 gctttgcata aaaaaccaac ctcggtgggt ttggcttcgg gtatggttgc tggccttgtg
     2341 ggaattaccc cgggtgcagg ccacgtttca ccgatggcgg cgctcgccat ttgttcactc
     2401 gctgcgctcg tttgctttac agccgttgtc gccaaacgcc gcttaggcta cgatgactct
     2461 ctcgatgtgt ttggagtgca tggcatgggc ggtttattcg gcgctttagc aaccggactt
     2521 ttcgcgagcg tgggcacacc gcacattggt ttgttctcgg gcggtgacgc acaacaattc
     2581 ttactgcaac ttttgggggc cggtgtgggt atcgccgttg cgggtgtagg cacaatcgtg
     2641 attgcgttca tcgtcgacaa aacggttggc ttgcgcgtct ctgtaaaaca ggaaacgttg
     2701 gggttagatc taagccaaca cggtgaaaca agcctgactc gggtttaatc caactactta
     2761 aggaggaaat tatgaaactt attattgcac tcatccagcc gcaccgttta gaagcggtga
     2821 agcgtgaact caacaagcgt gaaatctacc gcttaaccgt catcgaagcg atggggtatg
     2881 gccgccagaa gggccagctt caatactttc gcggtcaaga aatggaatcg gagctcatca
     2941 ataaaatcga gctacaaata ggcgtcaacg acgcctttgt cgaaccgacc atcgaagcga
     3001 ttatggcggg cgcacgcagc gactctgtgg gacaaattgg cg
//
LOCUS       DAOMPD010000324         3033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13119_length_3033_cov_2.402955, whole genome shotgun sequence.
ACCESSION   DAOMPD010000324 DAOMPD010000000
VERSION     DAOMPD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13119_length_3033_cov_2.402955"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLY93_10625"
     CDS             complement(<1..424)
                     /locus_tag="PLY93_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRP69974.1"
                     /translation="MPKRGVKNPISPFVAVLINPSSAGGLSLKRWAAYRDALHQKRVR
                     TTEYISVSEADFRERTRMLAKKFKRIAVVGGDSSLTIAAEELVKVNYKGVLQFMPAGS
                     VNDIVLDLRENTAQRQSAYLGFLKTQGKRKNFIGQANFG"
     gene            complement(436..801)
                     /locus_tag="PLY93_10630"
     CDS             complement(436..801)
                     /locus_tag="PLY93_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69975.1"
                     /translation="MPKPNAGKETAKPATSEVAQGDAKLFFVESIFPLLKEDELYKTL
                     TAKYGQRTGSSVGDTWRGAYTWDKADGFLVQWVEPYQKHGYTRSLYYLSRKIRDEIKN
                     DLQAWQFVREIKALDSLLK"
     gene            complement(871..1218)
                     /locus_tag="PLY93_10635"
     CDS             complement(871..1218)
                     /locus_tag="PLY93_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69976.1"
                     /translation="MRIYFLMMSAALTAALWAQAPRQPTTSGARPGTAPTTPTQPSAP
                     GRSPTQPTNPNTPTQPANGNNQNTQISGFDGVTWGTMYKDMKERFRVMASNSNANGND
                     NVEIIADTPDREI"
     gene            1309..2910
                     /locus_tag="PLY93_10640"
     CDS             1309..2910
                     /locus_tag="PLY93_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP69977.1"
                     /translation="MKIIIVGAALAGPTAAARAREINEKAEIILLERNTRVSYAMTGL
                     AHYLSGEVTLLDDLNREREDFFRDIYNIDVRPRTEVIHLDAKKHRITIETMGRREEMS
                     YDKLIFASGAGSLRPLGAPKDAENFRYFRTLDDLEAIRASLDANRKRFVVLGASSMGL
                     EALDGLVRGGAEVTLIEKKSGILPEYSRAITNLAHNSIAKKTRIITGFKEITFSQQDK
                     NIHTVTVDGEQIKTDFVVSAIGVKPRTEILKKAGVKLLANGAIPIDEKCRTNIENIYA
                     CSICAAVPVGTEHYWIAQAALSDKTAQVAGENAAGGKARLVVASASQIIRLPDIEVGR
                     VGIDRFGKNKVASVLVHASNIEPYMPAASPIALKLWYLKKNQQVVALEAVGHRIKARL
                     DAMATAIQGKLKLKDLAMLDFAYTPSMGTARDALNIAATVALQQANGITGIVTIDTVR
                     TQRKKYLVLDVSEKETQTEFHDLYIPLERLRSSHEVIQQKLKKTKAKTVALLSETGRR
                     GHLAFRILQSFGFNVQNISGGKASL"
BASE COUNT          755 a          746 c          742 g          790 t
ORIGIN      
        1 taccaaaatt cgcttggccg ataaaatttt ttcgtttacc ttgggtttta agaaacccca
       61 aatacgcact ttggcgttgc gccgtatttt cgcgcaagtc cagcacaatg tcgttgactg
      121 aacctgcggg cataaattgc aacacgcctt tgtagtttac tttgaccaac tcttcggcgg
      181 caatcgtgag cgaagagtcg ccacctacaa ccgcgatgcg cttaaatttt tttgccagca
      241 tgcgggtgcg ttcgcgaaaa tctgcctcgc ttacggagat atactcagtg gtgcgcactc
      301 ttttttggtg gagtgcgtcg cggtatgcag cccacctttt aagcgacaac ccgcccgccg
      361 agctggggtt aattaacacg gcaacgaagg ggcttattgg attttttaca ccgcgcttgg
      421 gcacccgtgt cttttttatt tcagtaagga atcgagcgct ttaatctcgc gcacaaattg
      481 ccacgcctgt aaatcgtttt taatttcatc gcggatttta cgcgatagat aatacagcga
      541 acgagtataa ccatgctttt gatatggctc tacccattgt accaagaagc catccgcctt
      601 gtcccaagtg tacgcaccac gccatgtgtc ccccaccgac gaaccggtgc gttggccata
      661 cttcgccgtg agcgttttgt aaagctcgtc ttcttttaat aaagggaaaa tactttcgac
      721 aaaaaataat tttgcatcgc cttgcgccac ttcgcttgtt gcaggctttg ctgtttcttt
      781 gcctgcattg ggtttcggca tcgactccac aggctttgct tccggttttt tgtaaaagac
      841 ataacggtaa taaatttcac gtcgacggat tcaaatttca cggtctggcg tgtctgcaat
      901 aatctcaacg ttatcgttgc cgttcgcgtt ggagttcgac gccatcacac gaaagcgttc
      961 tttcatgtct ttgtacatcg ttccccatgt aacgccatca aaaccagaaa tttgtgtatt
     1021 ttggttgttg ccgtttgctg gctgcgtcgg tgtgttgggg tttgtcggtt gagtgggact
     1081 tctcccgggt gcgctcggtt gcgttggtgt tgtaggggct gtaccggggc gcgctccgga
     1141 cgtagtgggt tgtcgtggcg cttgcgccca caatgccgct gtcagtgcag cgctcatcat
     1201 gagaaaataa atccgcattt tcacatttta ttatcggtag aataaaacaa actgcttagt
     1261 tttttccttg accgttgcag tttatgcggt attcgagtac aagcttttat gaaaattatc
     1321 attgtaggcg cagcgcttgc tggccccacg gcggccgcac gggcgcgcga gataaacgaa
     1381 aaagcagaga ttattttgct cgaacgcaac acccgcgtca gctatgcaat gaccggtctt
     1441 gcgcactatc tctcggggga ggttacatta ttagatgacc tcaaccgcga gcgtgaagat
     1501 tttttccgcg atatctataa catcgacgtg cgtccacgca ccgaggtcat ccatttggat
     1561 gcgaaaaaac accgaatcac tatcgagacg atgggccgcc gcgaagagat gagttatgat
     1621 aaactcattt ttgcctcagg tgcagggtcg ttacgccctt tgggggctcc taaagatgcg
     1681 gaaaactttc gttacttccg caccctcgac gatctggaag caattcgtgc gtcgctcgat
     1741 gcgaaccgca aacgctttgt cgtgctcggt gcaagttcca tggggctcga agccctcgac
     1801 ggtttagtcc gtggcggtgc ggaggtgacg cttatcgaaa aaaaatctgg aatcttaccc
     1861 gaatactcgc gtgcgatcac aaacttggcg cacaattcca tagcaaagaa aacacggatt
     1921 attactggtt tcaaggaaat tactttttca caacaggata agaatattca tacggtgacg
     1981 gtagatggcg agcagattaa aaccgatttc gtcgtttcgg ccattggggt caaaccacgc
     2041 acggaaattc tcaaaaaagc gggagttaaa cttttggcca atggggcaat ccccatcgac
     2101 gagaaatgcc gtacaaacat cgaaaatatt tatgcatgca gtatctgtgc cgccgtgccc
     2161 gtcggtacag agcactactg gatcgcgcaa gcggcgcttt ccgacaaaac agcgcaagtc
     2221 gcgggagaaa atgctgcggg cggcaaagca cgccttgttg tggcgagtgc atcccaaatt
     2281 atccgtctac cggatattga agtcggacgc gtcggcattg atcgctttgg taaaaacaaa
     2341 gtcgcaagtg ttttagtgca tgcaagcaat attgagccat acatgccggc cgcatcgcca
     2401 atcgcgctta agctctggta tcttaaaaag aaccagcaag ttgtcgcgct cgaagctgtc
     2461 ggtcatcgta tcaaagcacg cctcgacgcg atggcgacgg caatccaggg caagctcaag
     2521 ttaaaagatt tagccatgtt ggattttgca tacaccccat cgatgggcac cgcacgcgat
     2581 gcgctcaaca tcgctgcaac ggtcgccttg caacaggcca atggtattac tggcattgtt
     2641 accatagaca ccgttcgaac gcagcgtaaa aaatatctgg ttctggatgt ctcagaaaaa
     2701 gaaacacaga cagagttcca cgatttatat attcccctcg aacgcctacg ttcatcgcat
     2761 gaggtcatcc aacaaaagct gaaaaaaaca aaagcaaaaa ctgtcgcact cctgagcgaa
     2821 accggtaggc gtggtcattt agcattccgc attctgcaaa gttttggatt taatgttcaa
     2881 aatatttcag ggggtaaggc gtcgctttaa aatgaccgag atattgcaaa aaagctggtc
     2941 gcgagcattg ggtggcgata cgcagcgttt tcatcctgtc gatgtttttc gagcaggcga
     3001 agccaaccac catatatcga acactgcgta tcc
//
LOCUS       DAOMPD010000325         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13123_length_3032_cov_3.044004, whole genome shotgun sequence.
ACCESSION   DAOMPD010000325 DAOMPD010000000
VERSION     DAOMPD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13123_length_3032_cov_3.044004"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLY93_10645"
     CDS             <1..615
                     /locus_tag="PLY93_10645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HRP69978.1"
                     /translation="SLAGIPTRVEPFFVVLDCGGVGYGVRIPGNVYEAIIAGGYDTNQ
                     KKFSLVTRSIYSEDNAQLFGFLTENEAQLFDFVRSLSGFGPQAAMGILSTLGADGLLD
                     ALLQQNVAALVKVPGVGKTKAEKLCFEANTKKAKLEKLLKAPNQPKRADDASDEILTQ
                     ALLSLGYSTKEIQSASEKLKNKEKPATVTRENLQEWIRLFLQQL"
     gene            771..1268
                     /locus_tag="PLY93_10650"
     CDS             771..1268
                     /locus_tag="PLY93_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69979.1"
                     /translation="MYNYEGTGKTLFKAAKFNERRRAQKFFIQSARTHLIPLINAKTG
                     VIAIPSRYKFLFKLLKVCVPKEQLFSDVLQIKQKPFSKVANKTLSAAGRYARVYENLQ
                     WNTLSWRKQKFERYLLCDDVSTSGATLERAAYLLKAHLDLKDEQILFWALMYRPRLPL
                     EETTS"
     gene            complement(1258..2286)
                     /gene="rsgA"
                     /locus_tag="PLY93_10655"
     CDS             complement(1258..2286)
                     /gene="rsgA"
                     /locus_tag="PLY93_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRP69980.1"
                     /translation="MARVKGGVSTNDSSLKAVVAGVFGAYYHVILHLDRLDGQRFKAR
                     PRGKLRIDYREKNTGYNKLRETLLAIGDVVTMERDEKNADTLVITGVEPRRNAIHRAS
                     FGRQQCLAANIDGVIVMASILSPDFSLGFVERCLAEIHIAGVEAMLVVNKMDLRKEND
                     SDSETDSILSYYEKIGIPVFRESVRDALSPALAKTLQQGTWLILGQSGTGKSTLLNRV
                     LGKNLQSVGRVSLIDKGRHTTTNPALYFSEEHANLILIDVPGLREFGMHHRDAHEVKV
                     GFKDFQKLDCRFENCLHLKEPGCAVKAAVDAGEIPLFRYRSYMSMLDTVHENFKPRKG
                     DYWRGIKK"
     gene            complement(2288..>3032)
                     /gene="purQ"
                     /locus_tag="PLY93_10660"
     CDS             complement(2288..>3032)
                     /gene="purQ"
                     /locus_tag="PLY93_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRP69981.1"
                     /translation="QRRHGNLRSSGDSLTPGHSPQRRGGIMRTGVLTFPGSNCDADLI
                     DALKTDFHAEVELLWHTDTFEVRHDFYFIPGGFSYGDYLRTGALAAQAKSLASLRQAA
                     NENRPIVGICNGFQILCEAKFLPGVLIRNAKQNYICEWTPLKGDGDWKDTFPEDFHLP
                     ISHGEGNFLCSLEELERIEENGQIFLRYKENPNGALHNIAGVTSKNKRVLGLMPHPER
                     ALRAQKDSALSQKQFGKLFFEKLFTLASV"
BASE COUNT          806 a          745 c          696 g          785 t
ORIGIN      
        1 tctctcgccg gtattccaac gcgggtggaa cctttttttg tcgtgctcga ttgcggtggt
       61 gtcggatacg gcgtgcgcat cccggggaat gtgtacgagg cgattatcgc gggcggatac
      121 gacacgaacc aaaaaaagtt ttctttggtg acgcgcagca tttacagcga agacaacgcg
      181 cagctctttg gttttttaac cgagaacgaa gcgcaacttt ttgattttgt gcgttcgctt
      241 tcaggctttg gcccgcaagc ggcgatgggt attctttcga cgttgggcgc ggatggcctt
      301 ctcgacgcac tcttgcaaca gaacgtcgct gcgctcgtta aagtgccggg tgtgggaaaa
      361 acaaaagctg aaaaactttg ttttgaagcg aataccaaaa aggccaaact ggaaaaattg
      421 ctcaaagccc ccaatcaacc caagcgcgct gacgacgctt cggatgagat cttgacacaa
      481 gcgttgttat ctttagggta ctccactaaa gaaatacaga gcgcatcgga gaaattgaaa
      541 aacaaagaga aaccagcaac ggtgacgcgc gaaaatttac aagaatggat acgacttttt
      601 ctacaacaac tttagctgga cgcgcagcac atttattttt tcccgaaatg tgtctgcttt
      661 gcaacaaacg catagcgaca cgtcatttgt tttgctctcg ctgtctcaac gaaaaaccca
      721 tcacacgcgc aacgcaagta catgagaatt taatatggca acagcactat gtgtataatt
      781 atgaaggcac aggcaaaaca ctttttaagg cggcgaaatt caatgaacgc cgccgtgcac
      841 agaagttttt tattcaaagc gcacgcacac atttaatacc gctcattaac gcgaagacag
      901 gggtcatcgc aatcccgtcg cggtataaat ttttgttcaa actcctgaag gtctgcgtac
      961 ccaaagaaca acttttttcc gatgtactcc aaatcaaaca aaaaccattt tcaaaagtag
     1021 cgaacaagac attaagtgct gcggggcgtt acgcgcgcgt ctacgaaaat ttgcagtgga
     1081 atacgctctc ttggagaaaa caaaaattcg agcgttacct tttgtgcgac gacgtttcaa
     1141 cctcgggggc aacgctcgaa cgcgcggcgt accttttgaa ggctcatctc gacttaaaag
     1201 acgagcaaat tttattttgg gcgctcatgt atcgtccgcg cttgccttta gaagaaacta
     1261 cttcttgatg ccgcgccagt aatcgccttt gcgcggctta aaattttcgt gtaccgtgtc
     1321 gagcatcgac atatacgaac gatatctaaa aagtgggatt tcccctgcat cgacggctgc
     1381 cttgacagca cagccgggtt ctttgaggtg caggcaattt tcaaaacgac aatcgagttt
     1441 ttgaaagtcc ttaaatccca ctttaacttc atgagcgtcg cggtggtgca taccaaactc
     1501 gcgcaaccca ggcacgtcga tgagaataag gtttgcatgc tcttcgctaa aatagagcgc
     1561 tgggtttgtg gtggtgtgtc gtcctttgtc gatgaggctt actctgccta cgctttgcaa
     1621 atttttaccc aatacacggt taagaagcgt actctttccc gtccccgact gccccaagat
     1681 gagccacgta ccttgttgta atgtttttgc cagcgcaggc gaaagagcat cgcgcacgct
     1741 ttcacgaaaa acagggatgc caattttttc ataatacgaa agaatcgagt cggtttcgct
     1801 atcgctatcg ttctctttac gcaaatccat tttattgaca acaagcatcg cctcgacacc
     1861 agcaatgtga atttcagcaa ggcagcgctc gacaaaaccc aagctaaaat cgggagagag
     1921 tatcgacgcc attacaataa caccgtcaat gttagccgcc aaacattgct gccgtccaaa
     1981 cgaagcgcga tgtatcgcgt tgcgacgcgg ctcaacaccg gtgatgacca aagtgtcggc
     2041 gtttttttcg tcgcgctcca ttgtaacgac atcgccaata gcgagtaatg tttcgcgcaa
     2101 cttattgtag ccagtatttt tttcacggta gtcgatacgc aacttacccc gcggccgcgc
     2161 cttgaatcgc tggccatcga gtctatcgag gtgcaatatt acatgatagt acgcgccaaa
     2221 gacaccggcg actacagctt tcaacgaaga atcgtttgtg cttacgccgc ctttaacacg
     2281 cgccatacta aacagatgct aacgtaaaga gtttttcaaa aaataatttt ccaaattgct
     2341 tttgcgacaa cgctgaatct ttctgtgcgc gtaatgctct ttcgggatgc ggcatcaatc
     2401 ccaacacacg tttatttttc gatgtaacac cggcgatgtt gtggagtgcg ccgttgggat
     2461 tttctttgta tcgcaaaaat atctgtccgt tttcttcaat cctttccagc tcttcgagcg
     2521 agcaaagaaa attcccttcg ccgtgtgaaa ttggtaaatg aaagtcctcg ggaaatgtat
     2581 ctttccagtc gccgtcacct ttgagtggag tccattcgca aatgtaattt tgctttgcgt
     2641 tgcggattaa cacccctggc aaaaactttg cctcgcaaag aatttgaaaa ccattgcaaa
     2701 ttcccacaat gggacggttc tcgttcgccg cttgacgtaa cgatgcgaga gattttgctt
     2761 gggcggctaa agcccccgtg cgcaaataat caccgtacga aaaaccaccc gggataaaat
     2821 aaaaatcgtg acgtacttca aatgtgtcgg tgtgccagag aagttcaacc tctgcatgaa
     2881 aatcggtttt taacgcatcg attaaatccg cgtcgcagtt gcttcctggg aatgtgagta
     2941 cacccgtgcg cattatcccc cctcgccttt ggggagagtg gcctggggtg agggaatcac
     3001 cactacttcg caagtttcca tgacgtcgtt gt
//
LOCUS       DAOMPD010000326         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13124_length_3032_cov_2.889150, whole genome shotgun sequence.
ACCESSION   DAOMPD010000326 DAOMPD010000000
VERSION     DAOMPD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13124_length_3032_cov_2.889150"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1009
                     /gene="gspN"
                     /locus_tag="PLY93_10665"
     CDS             89..1009
                     /gene="gspN"
                     /locus_tag="PLY93_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HRP69982.1"
                     /translation="MTENELITEGLEESASLPTPPTENTRSRKKILLRAIPYLAGFFA
                     FLLGLLFFAPLESYAYLALRQIAGEGTRIEVIDLSLSSFGNFKVQGIQIPLAGTGGKQ
                     GLIKIAELKGKISLLNALFGDKYNSHAEAVIFSFTQGDLSFKIDALELNSNLTLEKSG
                     GTNKLLNGTFDFTAESAQLIYKEKKYLKEEIVIPFLKVTIKCRAQQGNIAVESADAIG
                     RLVNAQIRGSIQVGGQSNLNLNIILRPTDEFFEKYQDKDPRTLLKFAGILQDDNRIEL
                     NVRGPMNAPQVTPVTVKPALPTIPSNAPTP"
     gene            complement(1033..1641)
                     /locus_tag="PLY93_10670"
     CDS             complement(1033..1641)
                     /locus_tag="PLY93_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69983.1"
                     /translation="MKLKNIFIVQCALFFLALSFIFSTGKIHAASIDIQKIRLKGAQY
                     IKDLKYFQETGLVPARQTAIEMQIENFEQSLIGYRTAKPNERNGALRIVQGSYEKFNV
                     EFRALADIYREWVDKYQKQYYEKSSEPFADTTTKEKTIATVAVARQDEARASSAYNNR
                     NYAYAAHLYLRSLKHYHQAYQFRKWPQLVQLIVHTKEKPKNK"
     gene            complement(1660..2568)
                     /locus_tag="PLY93_10675"
     CDS             complement(1660..2568)
                     /locus_tag="PLY93_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69984.1"
                     /translation="MRRLKLFLQIVLLVGFPFSTTIFAAAPDQGFVEKIIGENRYFIE
                     FANTAVSNFGSPENEKSLKEANQHNFNANLYYLESNYVDAFKEIRLSQEILLDLYYDI
                     LRKRYIEDARALLDLNAPVIIQSKDKKSAHFLQLGYRDVEVSRQFRDMGYNYNRFLFS
                     NKIRYYIDGIKRARRAKRFAFLALIESKTPIEDKAEYQTQTWDEARNKDDREKIRDYE
                     RVKNSMVNQMNRKLYTDGMNFFLHHDDNYGLISGQKKSILKDAFKELSTKRPEKKKRN
                     PEYPAADQPEEKTPNAPTNENKPTKP"
     gene            2805..>3032
                     /locus_tag="PLY93_10680"
     CDS             2805..>3032
                     /locus_tag="PLY93_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69985.1"
                     /translation="MNRKTIYARLVPAHSTSASWHKTKLLRHALSALSIATLMTCGPK
                     ENNATMHQTDEIIEYRQDGAPFEGFIAYPKGK"
BASE COUNT          819 a          693 c          642 g          878 t
ORIGIN      
        1 aaagtcgaga gtttcaattt tcgacctgta atgaagaaac cggggattta cgacttcaac
       61 gtttcaattt caggcttcca gagaaaaaat gacggaaaat gaactcatca cagaaggatt
      121 agaagaatcg gcgtcattac ccacgccgcc caccgagaac acccgctcgc gaaaaaaaat
      181 tcttctccgc gccattccgt acctcgccgg tttctttgca tttcttttag ggttactttt
      241 ctttgcaccg ctggagtcgt acgcgtatct tgcgctacgc caaatcgcgg gcgaaggcac
      301 gcggatcgaa gtgatcgatt tatcgctgtc gtcattcggc aatttcaaag tacaaggcat
      361 tcaaattccg ttggcgggta cagggggcaa acaaggctta attaaaatcg cagaactcaa
      421 aggaaaaatt tcactcttga acgctctctt cggcgacaag tataactctc acgccgaggc
      481 ggttattttt tcattcactc aaggcgatct ttcgtttaaa atcgatgcgc tcgaactgaa
      541 ctcaaacttg acacttgaaa aatcgggcgg caccaataaa ttactcaacg gcacgtttga
      601 tttcaccgct gagtcggcac agctcatcta taaagaaaaa aaatacctta aagaagaaat
      661 cgtcatccct ttcctaaagg ttaccatcaa gtgccgagcg cagcagggaa acatcgctgt
      721 tgaaagcgcc gatgccattg gacgtctcgt caacgcacaa attcgaggat cgattcaagt
      781 ggggggacag agtaacttga atttgaatat catcttacgc cccaccgatg aattttttga
      841 aaagtaccaa gacaaggacc cacgcactct ccttaaattt gcaggcattc tacaagacga
      901 caaccgcatc gaactcaacg tgcgcggccc aatgaatgca ccacaagtga cgccggtaac
      961 agtgaaaccg gcgctgccaa cgattccttc taatgcgccg acaccgtaaa acgaggtaat
     1021 ctaaaatttg aattacttat ttttaggttt ttctttggtg tgtacgatga gttgaactaa
     1081 ctgcggccat tttctaaact ggtacgcctg atggtaatgc ttcaacgaac gtaaataaag
     1141 atgcgcggcg tacgcgtagt tgcggttatt gtacgcgctc gatgcacgcg cttcgtcttg
     1201 ccgtgcaacc gccaccgttg caatcgtttt ctctttggtg gttgtgtctg caaatggttc
     1261 gctacttttt tcataatact gtttttggta tttatccacc cattcgcgat aaatgtcagc
     1321 cagcgcacga aactcgacgt taaatttctc gtacgagcct tggacaattc gcaaagcgcc
     1381 attgcgttca tttggcttgg cggtgcgata accgattaag ctctgctcaa agttttcaat
     1441 ctgcatttca atcgcagttt gccgcgcggg cacaagacca gtttcttgaa aatattttaa
     1501 gtctttgata tactgtgcgc ctttaagacg aattttctga atgtcaatcg aggcggcatg
     1561 gatttttccc gttgagaaaa taaaactcag cgcaagaaaa aagagggcac attgaacgat
     1621 gaaaatattc tttaatttca ttctgttcac gtacctcccc tatggctttg tcggcttatt
     1681 ttcattcgtc ggagcatttg gtgttttttc ctcaggttga tctgccgccg gatactcggg
     1741 gtttctcttc tttttttccg gtcttttagt cgacagttct ttgaacgcgt cttttagtat
     1801 cgatttcttt tgaccggaaa tcaatccata attatcgtcg tggtgtaaga aaaaattcat
     1861 gccgtcggtg tagagcttac ggttcatttg attcaccata ctatttttta cacgttcgta
     1921 atcgcggatt ttttctcggt cgtctttgtt gcgcgcttcg tcccacgttt gggtttggta
     1981 ctccgccttg tcttcgatgg gggttttact ttcgatgagc gctaaaaacg caaaccgttt
     2041 tgcacggcgc gctcttttta taccatcgat ataatagcgt attttgttcg agaataaaaa
     2101 acgattatag ttatacccca tgtcgcggaa ctgtctcgac acttcaacgt cacggtagcc
     2161 caactgcaaa aaatgcgccg attttttatc tttcgactga ataatgacgg gagcatttaa
     2221 gtcgagcaat gcgcgcgcgt cttcgatata acgtttacgc aaaatatcgt aataaagatc
     2281 gagtaggatt tcttggctca accgaatttc tttgaacgcg tcgacgtagt tgctctcaag
     2341 ataatagagg tttgcgttga aattgtgttg attggcttct ttaagagatt tttcgttttc
     2401 gggcgaacca aaatttgaca ccgccgtgtt ggcaaattcg atgaaatacc gattctcgcc
     2461 aataattttt tcgacaaacc cttgatcggg tgccgccgca aaaatcgtag tcgaaaaggg
     2521 aaatcccact aagagtacga tttgtaagaa tagctttaat cgacgcattt ggctcctaaa
     2581 cgaaccctca taaaattcac aataaaatgt ttcttttgct gagaattctt ctctaatatc
     2641 gacaaaatga ctgaatgctt caagcaattt tctctataga gccgatgcgc caactcgcac
     2701 agcgtgtacg aagaggcaat ttttttgcaa attttctgga gtttactatt tgacgccatc
     2761 tcaaacaagc tttgatgcgc tgtatgtctt ggaaaatacg aagagtgaat cgcaaaacca
     2821 tttatgctcg gctcgtgccg gctcactcga cgagtgcatc ttggcacaag acaaaactct
     2881 tgagacatgc gttgagcgct ctatcaatcg ctactttaat gacatgtggt cccaaagaaa
     2941 ataatgccac catgcaccaa accgatgaga ttattgaata tagacaagat ggcgctccgt
     3001 ttgaaggctt tatcgcctac cccaaaggta aa
//
LOCUS       DAOMPD010000327         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13125_length_3032_cov_2.696339, whole genome shotgun sequence.
ACCESSION   DAOMPD010000327 DAOMPD010000000
VERSION     DAOMPD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13125_length_3032_cov_2.696339"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLY93_10685"
     CDS             <1..317
                     /locus_tag="PLY93_10685"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRP69986.1"
                     /translation="SGSAHTVLYVGGAAKISVRLQECFGLDVHPTLYSGKLLLTIELL
                     SPSRQPIATTQNLPSFWKSTYPEVRKELRGRYPKHFWPEDPLSMQGTTGTKKAADRKR
                     LI"
     gene            complement(314..724)
                     /locus_tag="PLY93_10690"
     CDS             complement(314..724)
                     /locus_tag="PLY93_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRP69987.1"
                     /translation="MQSSADIAQMLKSRGLRVSLQKLEIAKHVLGTHRHFTAEEIFRE
                     INTDFPRVSRATVFNVLNLFAENGLLRTLRARSDVLLYDSNTSEHDHVVDTATGKVYD
                     VELSPHERQDFLQSLWLKNPHLPKSNAASVVVHV"
     gene            859..2445
                     /locus_tag="PLY93_10695"
     CDS             859..2445
                     /locus_tag="PLY93_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRP69988.1"
                     /translation="MNIQPKKKHIMKSKKVSTSIVLISVVVLVSIAASTFAIQSFFHK
                     NTNSLIENTTTTLLKRVLQLFIETSISFEKEYDKARTDAERRASIVRWNRTIDTISYA
                     SNQHFGENIPQVRLIGSREIFGIQPMGTTTTAIQNNFESEAAKKIMHDKLPMYTVQEK
                     DFLRIAVPLPSNAHPGCAKCHQVPLDAPKLLGTANITVPLKMHKQQSLYYSLLVTGIV
                     VLVLGIVLVIILWFLKSKIFRPVAYLAKTTDVITAGDFTKKIVVFGSDVTREALTMLS
                     VLQKKFTETIFEIQQHSSLVMAAAEHVASTAQDLSRIAIAGSGQSISDGNIEKLIELI
                     ENNANSAKEADALAQESSSLTDRGSKAFHATFSALEEIISKVSIIQEISAQTNLLALN
                     ATIEAARAGEHGRGFAVVASEVSKLAEMSRNASKEISEVAQTSIAHSQEAQTLLDKIL
                     PSIQKTTENVVSIRERSDAQVPHVDEIHAVLNTLTRVTTNVASASEQLAASAEELTSQ
                     SENLNQRLAYFQVNTKNDGA"
     gene            complement(2608..>3032)
                     /locus_tag="PLY93_10700"
     CDS             complement(2608..>3032)
                     /locus_tag="PLY93_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69989.1"
                     /translation="EAQFLDVGLFDVLRKLTRNGIDVILSLLDQDYRRLPFPIPESTR
                     TVGEYLAMAHESLKLSAICVVCGEDAHHSQKLTLVGINAQGEPEYAPASFHSEVVTVG
                     TSQELAQNKLFVEKLQEFTQNIYEARCCNCHVVKDEPI"
BASE COUNT          864 a          704 c          688 g          776 t
ORIGIN      
        1 ccagcggcag cgcgcacacc gtgctttacg ttggaggcgc ggccaaaatt tcagtgcgac
       61 tgcaagagtg tttcggcctc gatgtgcatc ccactctata cagtggaaaa ctattattaa
      121 ctatagagct tttgtcgcca tcgcgccaac ccatcgccac gacacaaaac ttgccctcat
      181 tttggaaatc cacatacccc gaggtacgca aagagctgcg tggccgctac cccaaacact
      241 tttggccgga agacccgctt tccatgcaag gaacgacagg cacgaagaag gcagcggaca
      301 gaaaacgctt gatttaaaca tgcaccacaa cagacgccgc attacttttc ggcaaatgcg
      361 gatttttgag ccataacgat tgtaaaaaat cctgacgctc atggggggaa agttctacgt
      421 cgtatacttt accggtggcg gtatccacaa cgtggtcatg ctcggaggtg ttggaatcgt
      481 acagaagcac gtcgctgcgc gcgcgcaagg tacgcaaaag gccattttcc gcgaaaaggt
      541 tgaggacgtt aaaaaccgtt gcgcgcgaga cacggggaaa gtcggtattg atttcgcgga
      601 agatttcttc ggcggtaaaa tgacggtgtg tgccgagtac atgcttggca atctctagtt
      661 tttgcaacga aacgcgtaaa ccacggcttt tcagcatttg tgcaatgtcg gcagagcttt
      721 gcatagttaa taatatagtc acaaaaatag aaggcgtcta attttagata aaaaaagtaa
      781 ctttgagttg gatctcacca ccttgcaaat ctaaaaaaca aagcaaactt tttaagctgt
      841 atatgtattg cgtcgagaat gaatatacaa cctaagaaaa aacacatcat gaaatctaaa
      901 aaagttagta catctattgt tctcatcagc gttgttgtcc ttgtcagtat cgccgcatcc
      961 acatttgcta ttcaatcctt ttttcacaaa aataccaatt cgctaataga gaatacaacc
     1021 acgacacttc ttaagcgagt gctacaactt tttattgaaa cgagtatttc gtttgaaaaa
     1081 gaatacgata aggcgcgcac cgatgcagaa cggcgagcga gtatcgtgcg ctggaaccgc
     1141 accatcgaca ccattagcta cgccagcaac caacattttg gagaaaatat accgcaagta
     1201 cgcctcatcg gcagtcgtga aatttttgga attcaaccga tgggaacaac cacgacggcg
     1261 atccaaaata actttgaaag cgaggcagcc aaaaaaatta tgcacgacaa gctgcctatg
     1321 tatactgttc aagagaagga tttcttacgt atcgctgtcc cactcccttc caatgcacac
     1381 cccggctgcg caaagtgcca ccaagttcca ctcgatgcgc caaaactctt aggcactgca
     1441 aatattactg tgccgctaaa aatgcataaa caacagtctc tttattatag cctccttgta
     1501 acgggtattg ttgttttggt tttggggatt gttttagtaa ttattctttg gtttttgaaa
     1561 agtaagattt tcagacctgt ggcgtacttg gctaaaacga cagacgttat cacggcgggt
     1621 gattttacaa aaaaaattgt tgtttttggt tcggatgtaa cccgtgaggc cctcactatg
     1681 ctttctgtat tgcagaagaa attcaccgag acaatttttg aaattcagca acattccagc
     1741 ttggttatgg cggcggcaga acatgttgct tccactgcgc aagatttatc gcgaatagca
     1801 atagcgggct ccggtcaaag tatatcggat ggcaatatcg aaaaactgat tgaattgatt
     1861 gaaaataatg ccaatagtgc aaaagaagcc gatgcgctcg cgcaagaatc gtcgagctta
     1921 accgacagag gtagcaaggc attccatgcc acgttcagcg cactcgaaga aattatcagt
     1981 aaagtgtcta ttattcaaga gatttcagcg caaaccaact tgctggcact caacgcaacc
     2041 atcgaagctg cgcgcgccgg tgaacacggt cgtggttttg ccgtcgtcgc ttctgaggtt
     2101 agcaagcttg cagaaatgag ccgcaatgcc agtaaagaga ttagcgaagt ggcgcaaacg
     2161 tcaattgcgc attcgcaaga agcgcaaacg cttctcgata aaattttgcc gtcaattcag
     2221 aaaaccacag agaatgtcgt aagtattcgc gaacgctccg atgcgcaagt gccgcacgtt
     2281 gatgaaatcc acgcagtctt aaatacgtta acacgagtca caaccaatgt tgccagtgca
     2341 tcagagcagt tggccgcttc cgccgaagaa ctaacgtctc agtcggaaaa tttaaaccag
     2401 cgtttggctt attttcaagt gaacacaaaa aatgatggag cgtaagcacg gttagcctac
     2461 ggaacgtcag ttttgtttat ccctgtttta gtttcttgta tatgtgtagt gcatgatttt
     2521 cttaatggga cataatatta gatagttatc tcctaatgcg acataatatt ggatagttat
     2581 cccctaatac gacataatac tttatcgtta aatcggctcg tctttcacaa cgtggcagtt
     2641 acaacaacgc gcttcgtaga tattttgtgt gaactcctgc aacttctcta caaaaagttt
     2701 gttctgcgcc agttcttgtg aagtccctac agtcaccact tcgctgtgga acgacgcggg
     2761 tgcatattcg ggctcccctt gcgcgttgat gcccacgagc gtcagttttt ggctgtggtg
     2821 ggcatcctcg ccgcacacca cacaaatcgc cgacagtttg agtgactcgt gtgccatcgc
     2881 taagtactct cccacggtgc gcgtcgattc gggtatgggg aaaggcagtc ggcggtagtc
     2941 ttggtcgagc aaggataaaa tcacgtcgat accgttgcgg gttagtttcc gcaatacgtc
     3001 gaaaaggccc acgtccaaaa attgtgcttc tt
//
LOCUS       DAOMPD010000328         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13159_length_3027_cov_3.613728, whole genome shotgun sequence.
ACCESSION   DAOMPD010000328 DAOMPD010000000
VERSION     DAOMPD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13159_length_3027_cov_3.613728"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            581..1042
                     /locus_tag="PLY93_10705"
     CDS             581..1042
                     /locus_tag="PLY93_10705"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRP69990.1"
                     /translation="MERETKSVTHVPLPLEAAFEAFTNHNSYPKAGLGIQTKLIRPGN
                     SEESNGLGAVREIITPLGTAREEVVGINRPHTWDYHFLKWPMPYRHLSGRMSFREVSG
                     GTEVTWTSVIEVPDGIWGTIVGPVVQVFGDMFLKFLGSVLRDVAGQERNEK"
     gene            1374..1838
                     /locus_tag="PLY93_10710"
     CDS             1374..1838
                     /locus_tag="PLY93_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRP69991.1"
                     /translation="MATKYQGTPAEKQTLDLFIKFMRAHDSLENRTRRDFQNLDLTPA
                     QFGILETLCYLGPLKQCELASKLLTTAGNITRLLDTLQKIEYIERLPEPGDRRSFRIH
                     LTARGKKSVEKVFPVFLRSIQGSFNCLNTDEKKQLNQLLRKLGTHIKHSLNE"
     gene            1870..2253
                     /locus_tag="PLY93_10715"
     CDS             1870..2253
                     /locus_tag="PLY93_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRP69992.1"
                     /translation="MDSIISGFGKIGRYIFCIPLVAFGMNHLTQAPMMAGMVPIPGGV
                     FWVYFTGVAMLLGAIGIITNFKGLGALAAFLTGVLLLIYVFTIHLPGLINAKDVMSRM
                     MSLMMVLKDAGLAGGAWAIALQLKK"
     gene            2313..>3027
                     /locus_tag="PLY93_10720"
     CDS             2313..>3027
                     /locus_tag="PLY93_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69993.1"
                     /translation="MFDYKEDKLVWHGEAVNPLVHNAEDLLNKQFNYTGPFWLYSEKI
                     LTERLAILEKELPHFHFYHSVKSLPNIGILKRIQPNKNFGADVVSGGELYRALKAGFN
                     PDKIVFAGVGKTEAEIEYGIDKKIRAFHVESIAELKDIERIAEAKNVTAHIAFRLNPD
                     VEVDTHKHITTGKEENKFGMSDGDFTTGVTLLKKSKHLKLVGLQAHIGSQLFDAAPYI
                     QTAKYLHDKYAIVQKELAQK"
BASE COUNT          879 a          677 c          702 g          769 t
ORIGIN      
        1 tgtcaccgcg catacggtca atatacacaa aagttaggag attttcaaca tcctgttaga
       61 gggtaactat ccccattttc cggattatgt cgcattaggg ggtaactatc cacgcgagcg
      121 atttcttcaa aagtgagaaa aattgaccgt tttctggtct ttatcatata gagaccaata
      181 tgttaaagac atcaatcagt ttaccaaaag aatccgtcgt gacttggagc caaaaccact
      241 gggtaatcat gcagatgtat gcgcgatttt tgcgggtcgt tgagcggcag aaattcttgc
      301 agcccgcgtg gagccgattc gagcacactc atttattcac tcatagaaac ggcgaaggcc
      361 aacggtatgg agccgtgcga aagacaatca attctttgcc tgacaacggg tgttgtagga
      421 ccggtagacg gattcgtacc atactggcat aatccgtttt aaataaattg tgaagaatgg
      481 caatctaaaa gagaatagcc cttaagtcaa caatacacta taggtagagg attattttag
      541 tatatacggc ataatagttg tctaagcttt gtaagttatc atggaacgag agaccaaatc
      601 agtaacacat gtgcctctcc ctctggaagc ggctttcgag gcattcacca accataattc
      661 ataccccaaa gctgggcttg gcattcaaac caaacttatt cggccaggca attccgaaga
      721 atcaaatggt cttggagcag ttcgggaaat catcacgcca ctcgggaccg ctcgcgaaga
      781 agtcgtcggt ataaatcggc cccatacctg ggattatcac tttctaaagt ggccgatgcc
      841 ttaccgtcac ttgagtgggc gcatgtcatt tcgtgaggtg tcgggtggaa ccgaggtaac
      901 atggacatcg gtgattgaag tgcccgatgg catttggggc acgattgtcg gtccagtagt
      961 gcaggtattt ggagatatgt ttctgaaatt tctgggttca gtgctgcgcg acgtagcagg
     1021 acaagaaagg aatgaaaaat aatctttttt tattcaataa ctgtaaataa agaataattt
     1081 taggctatcg aaagcatgct taacacttca agccagaggg cggtaaagcc tacgacccgc
     1141 cgctgattct ttcacttttg atttatggct accaccgtgg gtggcgcagt tcgcgcgaat
     1201 tgcagcgtgc ttgtgaagaa aacacggcca tgcgttatct ggtgggtgat cataaaatta
     1261 aggcgagcgc ggcgaacgat caaacatata cgcggcaaaa aattagaggc gcacaaactc
     1321 taaaatagtt gccaacttta ttgttgatat atatattgtt catacatcaa caaatggcga
     1381 caaaatatca aggaacaccc gccgaaaagc aaacgcttga tttgtttatc aagtttatgc
     1441 gcgcgcacga ttcgcttgaa aaccgcacgc ggcgcgattt tcaaaatctt gatttaacgc
     1501 ctgcacagtt tggcattctc gaaactttgt gttatttagg gccgctaaag caatgtgaat
     1561 tggcgtcgaa gcttttgacg acggccggca acattacccg ccttctcgac accctgcaaa
     1621 aaatcgaata catcgagcgc ttgcccgaac ccggcgatcg ccgttcattt cgcattcatc
     1681 tcacggcacg aggcaaaaaa tcggttgaaa aagttttccc cgtatttctt cgtagcatac
     1741 aaggctcgtt taactgtctg aacaccgacg aaaaaaaaca gttaaaccaa ctgttgcgta
     1801 aactgggtac gcacattaag cattcattaa atgaatgaaa ataaaaattg aaaacaaaag
     1861 gagattcaaa tggattcaat tatttcaggt tttggtaaaa tcggtagata cattttctgt
     1921 attccgctgg ttgcgtttgg aatgaaccac ttaacacaag cgccgatgat ggctggcatg
     1981 gtccccatac ccggcggcgt attttgggtt tattttaccg gtgtggcaat gcttttgggc
     2041 gcgattggta tcatcaccaa cttcaaaggt ttaggcgcgc tcgcggcgtt cttgaccggc
     2101 gtgttgctgc tcatctatgt attcactatc cacttgcccg gtctcatcaa cgccaaagat
     2161 gtaatgagcc gtatgatgtc gctcatgatg gtcttaaaag acgcaggttt agccggcggt
     2221 gcttgggcga ttgctcttca gttgaaaaag tagcccacaa cttcaaagtt aaagagtcgc
     2281 tgtcatggag cgactttttt tttaggctac acatgtttga ttataaagaa gacaaactcg
     2341 tctggcatgg tgaggctgta aatccccttg tgcataacgc cgaggattta ctgaacaaac
     2401 aattcaacta caccggccct ttttggcttt acagcgaaaa aattttaacc gagcggttgg
     2461 caatcctcga aaaagaattg ccgcattttc atttttacca ctcggtaaag tcattgccca
     2521 acatcggcat cttgaaacgc attcaaccga ataaaaattt cggcgccgac gtggtgtcgg
     2581 gcggtgagct ctaccgtgcg cttaaagcgg gatttaaccc cgacaaaatt gtgtttgcgg
     2641 gtgtgggcaa aaccgaagcg gagattgagt acggcatcga caaaaaaatc cgcgcattcc
     2701 acgtcgaaag tatcgccgag ctcaaagata tcgagcgcat cgccgaggca aaaaatgtga
     2761 ctgcgcatat tgcgttccgc ctaaaccccg atgtcgaggt cgatacgcac aagcacatca
     2821 ctaccggcaa agaagaaaat aaatttggca tgagcgacgg cgattttact acgggggtaa
     2881 cgctcctaaa aaaaagcaaa cacctcaaac tggtgggact acaagcgcac atcggctcgc
     2941 agcttttcga cgcagcgccg tatatccaaa ctgcaaaata cttgcacgat aaatacgcta
     3001 ttgtccagaa agaactcgcg caaaaaa
//
LOCUS       DAOMPD010000329         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13160_length_3027_cov_2.625505, whole genome shotgun sequence.
ACCESSION   DAOMPD010000329 DAOMPD010000000
VERSION     DAOMPD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_3027_cov_2.625505"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLY93_10725"
     CDS             complement(<1..585)
                     /locus_tag="PLY93_10725"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRP69994.1"
                     /translation="MKNFLILRKQFFTVFLLLAIFFVACTKKKEAEVWYCPMHTHYQS
                     DHAGKCPICGMNLVKKENTDITGNKEEQKDEHKSHAEQATPTENSITVSADAQQRAGI
                     VTTRPSKRQLSMTLTLAGEVAYEPDIYAATIEYRQLVAAGQTLDASMGGFNLTQSALL
                     RLRQMGLSKDEIHQYAHSNTAASRLITGTGAGYAL"
     gene            complement(582..1829)
                     /locus_tag="PLY93_10730"
     CDS             complement(582..1829)
                     /locus_tag="PLY93_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP69995.1"
                     /translation="MRYLIYIFAIFISPLVAADAGTYFQIALEKNARLAEEAQLAAAT
                     REEHLRESIFNNNPELSVGLMNVPTRTFPAINRDSMSGLSVGISQRLALPWEAHFRKA
                     EAEWRATNAALAVELQKAVLRYDVYEKFNAVLFYTKRAKNLANTKRLIVATLKVLSVP
                     RKDARNIASQILEARASLATQENTILENEYELEKAWLDLSALCGQNLERRSGEDALKA
                     WEDLSLLTLPKSEDITAALLYKKSAQEVNTAEAMASLARASTFPEVTLSASYLFRQQV
                     PGSSMNDDMISVAASTPFPFFYSLKNRHDIAAINNRVEAARAHLLETERQLTAQIAAE
                     KTRFHALVKAAENSTKAILPAHSSAHHSQLTTLNLTGSSAAEALIAYRMYLTADEEHL
                     KQVRDAHAAFHRLHYLLAGGLTP"
     gene            complement(1891..2289)
                     /locus_tag="PLY93_10735"
     CDS             complement(1891..2289)
                     /locus_tag="PLY93_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69996.1"
                     /translation="MRKSVVGYFLKSATFAFALPLSLAAAPLCNTCSPEIPFTQNIAD
                     TTQAKTHACCEKKSQIQNIAHKTRCLSCLETPSTPATETKASPIIPLNTVLPSVVFTL
                     KTDFVYFSITQFYPPLSSSPPFILLKKILL"
     gene            2421..>3027
                     /locus_tag="PLY93_10740"
     CDS             2421..>3027
                     /locus_tag="PLY93_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRP69997.1"
                     /translation="MKYFIPSALLGFLSLLTIPTVNADAPFRKISRIELKPVFHRRFK
                     QPVFVTPYPGRIAACPDAYAIVEKRGVVRIESMDQKCGLVLADLRDRVAAPTLEEGLL
                     GLAFPPNFRESGSYFVYYSAANPRRTILARLDMTGKMGVGKNRVTELLTIRQPISNHN
                     GGMLMFGPDGYLYLGVGHGGSGGDPRGYAQNTASHLGKLLRI"
BASE COUNT          683 a          700 c          835 g          809 t
ORIGIN      
        1 gagagcataa cctgcccctg tgccggtgat gagacgcgac gccgctgtat tcgagtgcgc
       61 gtattggtgg atttcatctt tggacaagcc catttgccgc aagcgcaaga gcgcactctg
      121 cgtgagatta aagccgccca tcgacgcgtc gagtgtttgt ccggcggcga cgagctggcg
      181 atactcaata gtcgcggcgt aaatatcggg ttcgtacgca acctctcccg caagtgtgag
      241 cgtcatcgac agttggcgtt tgctcgggcg cgtggtgaca atccctgcgc gttgttgcgc
      301 gtccgcactc acggtgatgc tgttttctgt tggggttgct tgctcggcat ggcttttatg
      361 ctcgtctttc tgctcttctt tatttcccgt tatgtcggta ttttccttct tcaccaagtt
      421 cattccacaa atggggcatt tacccgcgtg gtcgctttgg taatgtgtgt gcatcgggca
      481 ataccacact tcggcttctt tttttttcgt gcacgcgacg aaaaaaatag cgaggagtaa
      541 aaacacggta aaaaattgtt ttcgtaaaat taaaaaattc ttcatggtgt taatccccct
      601 gcaagaagat aatggagtcg atggaacgct gcgtgcgcat cgcgcacttg cttcaagtgc
      661 tcttcgtcgg cggtcaggta catacggtat gcaatgagcg cttcggctgc gctcgatcca
      721 gtgaggttga gtgtggtgag ttgcgagtgg tgcgccgagc tgtgtgcagg taagatggct
      781 ttcgtggagt tctcagcggc tttgacgagc gcatgaaaac gtgtcttttc ggcagcgatt
      841 tgcgcggtaa gttggcgttc ggtttctaga agatgggcgc gcgctgcctc gacacggtta
      901 tttatggcgg cgatgtcatg gcggtttttc aaactataga agaatgggaa cggtgtcgat
      961 gccgccactg aaatcatgtc gtcgttcatg gagctaccag gtacttgttg gcggaacaaa
     1021 tacgatgccg agagcgtcac ctcggggaat gtggacgcgc gtgccaaaga cgccatcgct
     1081 tcagcggtgt tgacctcttg cgcagatttc ttgtaaagta gcgccgctgt aatatcctcg
     1141 cttttgggta aggtgaggag cgataaatct tcccatgcct ttagcgcatc ttcgccgctg
     1201 cggcgttcta aattttgacc gcacagcgcc gacaaatcga gccatgcttt ttcaagctcg
     1261 tactcgtttt ccaaaatggt gttttcttgc gtggcgagcg aggcgcgcgc ttctaaaatt
     1321 tggctggcaa tgttgcgtgc atcttttctg gggacgctca acaccttgag tgtcgcgacg
     1381 atgagtcttt ttgtattggc caaatttttt gcgcgcttcg tgtagaaaag gactgcgttg
     1441 aatttttcat acacgtcgta gcgtaacacc gctttttgaa gctccacagc taacgccgca
     1501 ttggtggcac gccattcggc ttcggccttg cggaagtgcg cctcccacgg gagcgccaag
     1561 cgctgtgaaa taccgacgct caaccccgac atcgagtcgc ggtttatcgc aggaaacgtc
     1621 cgtgtcggca cattcatgag tcccacagaa agttcggggt tattgttaaa aatactctcg
     1681 cgtaaatgct cctcacgcgt ggcggcggct aattgagcct cctcagcaag tcgcgcattt
     1741 ttttcgagcg ctatttggaa ataggtacca gcatcggcgg caacgagagg cgatataaag
     1801 attgcaaaaa tataaattaa gtaacgcata aatcctccta aaaagtttgg gtgttatttc
     1861 atacgcgctg cgcacgcagg gaatgactta ctataagagg atttttttta atagaataaa
     1921 gggtggagag gaagagagcg gtggatagaa ttgggttatt gaaaaataaa cgaagtcggt
     1981 ctttagggta aataccactg aaggcagaac ggtattaagc ggtataatcg gcgatgcttt
     2041 agtttcggtg gcgggggtag aaggcgtttc aagacacgaa aggcagcgtg tcttgtgtgc
     2101 gatgttttga atttgggatt ttttttcgca gcacgcatgc gtttttgctt gagtagtgtc
     2161 ggctatattt tgtgtgaatg gaatttcggg agaacaagtg ttgcaaagtg gcgccgccgc
     2221 taatgaaagc ggtaatgcaa aagcaaacgt cgcgctcttg agaaaataac cgacgacact
     2281 tttacgcatc aatacttaat aatagtaaat aaaaaagcta tcggcaagta aaattcctga
     2341 gcgagttggc gcacaggctc gtcatagact ccttgataga ctccttgcca cgctggccgc
     2401 aactttatac attgtgttta atgaagtatt ttattccaag cgccttactc ggatttctct
     2461 cgttattaac tatacccact gtgaatgcgg acgccccgtt ccgaaagata tcacgcattg
     2521 aacttaaacc ggtgttccat cgtcggttta agcaaccggt gtttgtgacg ccgtaccccg
     2581 ggcgcattgc ggcatgcccc gatgcatacg cgatcgttga gaagcgggga gtcgtccgta
     2641 tcgaaagcat ggatcaaaag tgcggactgg tgctcgccga tttgcgcgac cgtgtcgccg
     2701 cccccacgtt agaagaaggg ctcttggggt tggcgtttcc tccgaatttt cgggagagcg
     2761 gcagttattt tgtgtattat tcggcggcaa accctcgccg cacgatatta gcgcgtttgg
     2821 atatgacggg taaaatgggt gttggtaaaa accgcgtcac cgagttatta actatacgcc
     2881 aaccgattag caaccacaac ggcggtatgc tgatgtttgg ccccgatggt tatctttacc
     2941 tcggcgtggg ccacggtggc tcagggggcg atccgcgtgg atacgcgcaa aacaccgcgt
     3001 cgcatttggg caaactttta cgcatcc
//
LOCUS       DAOMPD010000330         3010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13271_length_3010_cov_3.165482, whole genome shotgun sequence.
ACCESSION   DAOMPD010000330 DAOMPD010000000
VERSION     DAOMPD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13271_length_3010_cov_3.165482"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1695)
                     /locus_tag="PLY93_10745"
     CDS             complement(115..1695)
                     /locus_tag="PLY93_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HRP69998.1"
                     /translation="MKWMRLKALPLFIIVSVIFSCVRRATEIEPTTLTYHLPSDPINL
                     NPVISEDAYSSAIQSRIFESLVERDPKTLLFRGNIAKSWNVGADKLTYTFHLRDNIFF
                     HDDTPLTATDVLFSYKMLMDDKIPNAHKKVYYKDVESLNAPDAHTVVFRMKRPYAFAL
                     EHLGGFEIIPKHVYEVGNFMTDERNFRGPVGSGPYRFVEWKNGVRVVLTRFEKYWGAT
                     PAIRKIEYQIIKNDAVALQALKKGDIDSYNLKPLQWTRQTNSEKFNRDFRKIKYLAPS
                     YRYVGYNMRTFPFNDRRVRLAMAHLMDLPRIQKTILEGLAEITTGPFLPQSLQYNKSL
                     PPLAYNPQKALLLLKEAGYTPNSKGMLEKDGNVLEIEFMVPAVGGFYDQFVSIIKEDF
                     YKVGILLTPRKLEFQTLLEKINKREFHAVMLGWSSGIESDPYQLWHTSQRATGHNFTG
                     FGNTESDALIEKARVTFNVTERNKLYHRFHEIVYQEQPYTFLYVGYALIALNRRFTNV
                     NVYPAGLDMLEWKIEGNE"
     gene            1792..2373
                     /locus_tag="PLY93_10750"
     CDS             1792..2373
                     /locus_tag="PLY93_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP69999.1"
                     /translation="MELIASILVLAVTAALILLLNYYRRLLNDGRREYPREELIYAHN
                     FILNEWQRLIHGDVALSYAGVAIGALLGYMLSYTGGVYGEHYESAFFHSAIIPALWFI
                     GQPYLKEKADELGWPIAIRKFIQNDAAFFFALSSTIASQSLVSYGFYHALSFLWIAFN
                     FVVIVALLLYYFYKCERVEKGGLPDRPPRENKI"
     gene            2398..2865
                     /locus_tag="PLY93_10755"
     CDS             2398..2865
                     /locus_tag="PLY93_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP70000.1"
                     /translation="MAQSDARGIQYVLFKVGVETYAVEIAETQEVLRYQEPRRIPHAP
                     SHVLGVINLRGQIIPIVGLRERFSIEPIPPTAETRIIVVASEGKLTGVVCDSVERVLY
                     IPIENIEANPDFANSGTRSSIHGVAHQEGEEGVIFLLAISTVTADIPVVPTSA"
BASE COUNT          752 a          658 c          747 g          853 t
ORIGIN      
        1 ccggcggcgg ggggtcatat caaagaatct catctatatt ataaatacca gaaaacgctc
       61 gatttttctc acttttggaa aaaaaagtgc aaaaactcaa aaaaaatgaa gtttttactc
      121 attcccttca attttccatt cgagcatatc caaccctgca ggatacacgt ttacattcgt
      181 gaagcggcgg ttcaatgcaa tgagcgcata accgacgtag agaaatgtat agggctgttc
      241 ttgatagaca atttcgtgga agcggtggta gagcttgttg cgttcggtga cgttaaacgt
      301 gacacgcgct ttttcaatga gtgcgtcgct ttcggtgttg ccaaaaccgg tgaaattgtg
      361 gccggtggca cgttgcgacg tgtgccaaag ttggtatgga tccgattcga tgccggatga
      421 ccaaccgagc atcactgcgt ggaactcgcg tttattgatt ttttccaaaa gtgtctggaa
      481 ttccagtttt cgtggcgtaa gcaaaatacc cactttgtaa aaatcttctt taataataga
      541 aacaaattga tcgtaaaaac caccgaccgc aggcaccata aattcaattt ccaaaacgtt
      601 gccatctttc tcaagcatac cttttgagtt aggggtgtat cctgcttctt tcaaaagaag
      661 aagtgctttt tgtggattat acgcgagcgg tgggagcgat ttattgtatt gcaagctctg
      721 cggcaaaaat gggccagtgg taatttctgc aagcccttcg aggatggttt tttgaatgcg
      781 tggtaagtcc atcaagtgcg ccatcgcaag tcgtacacgt ctgtcgttaa aagggaaggt
      841 gcgcatgttg tacccaacgt agcggtagct gggggcgaga tattttatct tacgaaagtc
      901 gcggttgaat ttttctgagt tggtttgccg tgtccattgc agtggtttta agttgtagct
      961 gtcgatgtcg ccttttttta aggcttgcag cgcgacggca tcgttcttga taatttggta
     1021 ttcaatttta cggatggcgg gggttgctcc ccaatatttt tcgaagcgtg tgagtacaac
     1081 acgaacgcca tttttccatt cgacaaaacg atacggccca ctccccacag gcccacggaa
     1141 gttgcgttcg tcggtcataa agttaccaac ctcgtacaca tgtttgggga taatttcaaa
     1201 accacccaaa tgttcgagcg caaatgcata aggtcgtttc atacgaaaaa caacggtgtg
     1261 tgcatcgggt gcgttgaggg attctacatc tttataataa acctttttgt gcgcgttggg
     1321 aattttatcg tccatcaaca ttttatagct gaaaagtaca tccgttgcag tgagcggcgt
     1381 atcgtcgtgg aaaaaaatat tgtcgcgcaa atgaaacgtg tatgtgagtt tatccgcacc
     1441 gacgttccac gacttcgcaa tgttgccgcg aaaaagcagc gtttttggat cgcgttcgac
     1501 caaactttca aaaattcgcg attgaatggc cgacgaatac gcgtcctcgg aaattaccgg
     1561 attgagatta atgggatcgc ttggcaagtg gtatgtaagc gttgtaggtt caatttctgt
     1621 cgcacggcgc acgcaagaga agataacgct cacaataata aaaagaggca atgcctttag
     1681 gcgcatccat ttcatacggg atgtccttca cgtttgagcc cgttcgacaa tacaaatagt
     1741 taaaattttg aaatgtgatt gaatggctgc gcgaatgctt gaatgtggtt catggaactg
     1801 attgcgtcaa tccttgtact tgccgtcacg gctgcgctta ttttgcttct gaattattac
     1861 cgtcgtctct taaacgacgg gcggcgtgag tacccgcgtg aagaactcat ttatgcgcat
     1921 aattttattt taaacgaatg gcagcggctc atccatggcg acgttgcgct cagttacgca
     1981 ggggttgcta ttggtgcgct tttggggtat atgctcagtt ataccggtgg tgtgtatggc
     2041 gagcactacg agagtgcttt ttttcattcg gcgattatcc ctgcgttgtg gtttattggc
     2101 caaccttatc tcaaagaaaa agcagacgag ttaggctggc caatagctat tcgtaagttt
     2161 atccaaaatg acgcagcgtt tttctttgcg ctgtcgagca caatcgcttc gcaatcgctc
     2221 gtttcgtatg gcttttatca tgcacttagt ttcttgtgga ttgcttttaa ttttgtggtc
     2281 atcgttgcgc tcttgctgta ttatttctat aagtgcgaac gtgttgaaaa agggggattg
     2341 cccgatcgac cgcctcgtga aaataaaatt taagtattta aagaaggaaa tccaccagtg
     2401 gcacaaagcg acgcacgcgg cattcaatat gttttgttta aagtaggcgt tgaaacctac
     2461 gcggtcgaaa ttgcagaaac tcaagaagtg ttgcgttatc aagagccccg ccgcatcccc
     2521 cacgcaccat cacatgtttt gggagtgatt aacttgcgcg gtcaaattat tccgattgta
     2581 ggcttgcgtg aacgtttttc cattgagccg attcccccca ccgcagaaac gcgtatcatc
     2641 gttgtcgctt cagaaggcaa gcttaccggc gtggtgtgcg attctgtcga gcgggttctt
     2701 tatatcccca ttgaaaacat cgaagccaat cccgattttg ccaattcggg cacccgttcg
     2761 tcgatacacg gggtggcgca ccaagagggc gaagagggag ttatttttct cctcgccatt
     2821 agcactgtga cggcagacat ccccgttgta cctaccagcg cctaacgaca ccgacacgca
     2881 ctttatgcgc gcagcgtatc aaaatgcgct gaaaggcgtc ggtaacagcg accccaatcc
     2941 agctgtgggt gcggtgattg tgtcgccgga tggtatagtt ttggccgagg gtgcaaccga
     3001 gcgcgcgggg
//
LOCUS       DAOMPD010000331         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13290_length_3006_cov_3.487292, whole genome shotgun sequence.
ACCESSION   DAOMPD010000331 DAOMPD010000000
VERSION     DAOMPD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13290_length_3006_cov_3.487292"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLY93_10760"
     CDS             <1..427
                     /locus_tag="PLY93_10760"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP70001.1"
                     /translation="EDALQDAFERLLHAPRVNDANDATAWLYRVAHNRAIDVLRAQKK
                     TIAGEEYLFNEKSTPATQEIRLLRKERDALIEKAAQQFDTKGRGTLAYHLLRETPLTP
                     EKMAHALGMAPRTCRRFVDNLFIYIQNELQKNGYTREDL"
     gene            470..1222
                     /locus_tag="PLY93_10765"
     CDS             470..1222
                     /locus_tag="PLY93_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70002.1"
                     /translation="MEKKIDKHLSLKDFLFFHHRVLDVSGGILFERGNFIAQSDANRI
                     REHLMICEECRQKAIEWARENTPAKKKVPANFYWRRYTPVAAVLLLSVGAFFFFNQNK
                     TTTGVVVRSTNEPSHFLVNHKNNAIGHIKMAPYSEINIPTHNEPFHKVRLSEGEAQVT
                     IERTGPSYLDIVGDRGVVRVSWLGKKSETRNEPVLQFSIVDRDERRGSPDFFVQVEHG
                     ELAIENTQSGKSAFRSGDAFTVSKNSEITPVY"
     gene            1325..1915
                     /locus_tag="PLY93_10770"
     CDS             1325..1915
                     /locus_tag="PLY93_10770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRP70003.1"
                     /translation="MIAVGKKAPDFKATALVGKDFKDVSLGDYKGKYVVLFFYPLDFT
                     FVCPTEIIAFSDKAAEFEKIGAQVIGCSVDSKFSHLAWSDVPRDKGGIGEVKYPLLAD
                     ITKDIARNYGVLIEDEGVALRGVFIIDGKGVLKSATINNNNVGRNIDEVLRTVQADQF
                     SESHAGEVCPANWAPGKDSMKADTKGAQAYFAKHGK"
     gene            complement(1973..2620)
                     /locus_tag="PLY93_10775"
     CDS             complement(1973..2620)
                     /locus_tag="PLY93_10775"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HRP70004.1"
                     /translation="MKSYNHPRVFVHTLAVFSLTTFVFTGCNNYGLVDMLENPAGFVS
                     CKNEGPCYIFVATSGGGFNGNLGGIAGADQICNSSPLKPNSGKYKAVLVGSDANSRKV
                     CVSPDCTGTDEAVDWVFHPNTTYVNILGQTIGTTSGNGIFLSILNPVSTSATPAEVWT
                     GLNANWQDFNTCNNWTTSEVAYYGAGGFANSIGLLFSGASAQTCDVLRNLYCTQQ"
BASE COUNT          866 a          707 c          672 g          761 t
ORIGIN      
        1 cgaggacgcc ctacaagatg cgttcgaacg cctcttgcat gcccccagag tcaacgatgc
       61 gaacgacgcc accgcatggc tttaccgcgt ggcgcacaac cgcgccattg acgtcttacg
      121 cgcacaaaag aaaaccattg caggtgaaga atatctcttc aacgaaaaaa gcacaccggc
      181 aacgcaagaa atccgacttt tgagaaaaga acgcgatgcg ctcattgaaa aagcggcgca
      241 acaattcgac acaaaagggc gcgggactct cgcgtaccac ctgttacgtg aaacaccact
      301 cactccagaa aaaatggcgc acgcgcttgg tatggcaccg cgcacttgcc ggagattcgt
      361 cgacaacttg tttatttata tacaaaatga actgcaaaag aacggctata ctcgagagga
      421 tttataaatt tttggcaaat ttttttagga cgaacgttat aatactggta tggagaaaaa
      481 aatagacaag catctttcgc tcaaagattt tttatttttt caccaccgtg ttcttgatgt
      541 ttcgggtgga atcctctttg agcgcggtaa ctttatcgct caaagcgatg caaaccgtat
      601 tcgagagcac ctgatgattt gtgaagagtg ccgccaaaaa gccatcgagt gggcgcgtga
      661 gaataccccc gcaaagaaaa aagtaccagc aaatttttat tggcggcgtt atacacccgt
      721 ggctgcggta cttcttttaa gcgtgggtgc attctttttt ttcaatcaaa ataaaacaac
      781 aaccggcgtg gtggtacgct ccactaacga gccatcgcat tttttagtga accacaaaaa
      841 caacgcaatc ggtcacatta aaatggcgcc gtatagcgag ataaacatac ctacgcacaa
      901 tgagcctttt cataaagtac gtttatccga aggcgaagca caagtcacga ttgagcgtac
      961 cggcccttcg tatttagata tcgtcggcga ccgaggtgtt gtgcgcgttt catggttggg
     1021 caaaaaatca gaaacacgca acgagccagt tttgcagttt tcgatcgtcg accgcgacga
     1081 gcggcgcggc tcgcccgatt tttttgtgca agtcgaacac ggcgagttgg cgattgaaaa
     1141 tacccaaagc ggcaaaagcg cattccgttc gggggatgca ttcaccgttt ctaaaaatag
     1201 cgaaatcacg cccgtgtatt gaacctcggt ggcatgggtg taattttatt tgacgctctc
     1261 actaaaaact attatattaa cagagtataa atcaatacta agtataaaaa ctaaaagagg
     1321 taatatgata gcagtaggta aaaaagctcc tgattttaag gcgaccgcct tggtcggcaa
     1381 agatttcaaa gacgtctcgc tcggcgatta caaaggaaag tacgttgtgc ttttctttta
     1441 tccactcgac ttcaccttcg tgtgccccac tgagattatc gctttctccg ataaagcggc
     1501 agaatttgaa aaaatcggtg cacaagtcat cggttgctcg gtcgacagta agtttagcca
     1561 cttggcttgg tcggatgtcc cccgtgacaa aggcggcatc ggcgaagtga agtatccact
     1621 cttggccgac atcaccaaag acattgcacg caactacggc gttttaatcg aagacgaagg
     1681 tgtggcattg cgcggtgtgt tcatcatcga cggcaaaggc gtgcttaaat cggcgacgat
     1741 taacaacaat aacgtgggcc gtaacatcga cgaagtgttg cgcaccgtac aagccgacca
     1801 attctcagaa tcgcacgccg gtgaagtttg tcccgcaaac tgggcacccg gtaaagattc
     1861 gatgaaagcc gacacgaaag gcgcgcaggc gtatttcgca aaacacggca agtaaaaatt
     1921 ttacactatt tttcagtcgc gtccgcgact gaaaaatagt gtgcttattc gtttattgtt
     1981 gtgtgcagta aagattgcga agcacatcgc acgtctgagc agacgcacca ctgaacagta
     2041 aacctatact attggcaaac cctcctgcac cataatatgc gacttcactc gttgtccaat
     2101 tattacatgt attaaaatct tgccaatttg cattcaaccc cgtccaaact tcagcgggtg
     2161 ttgccgatgt agacaccgga ttgagtatag ataaaaaaat tccattgcca ctggtagtac
     2221 caatcgtttg acccaaaata tttacatacg tggtgtttgg atgaaagacc caatctaccg
     2281 cttcatcagt acccgtacag tctggactca cacacacctt acgcgaatta gcatcgctac
     2341 caaccaacac cgctttgtat ttaccgctgt tcggttttag aggcgagcta ttgcaaattt
     2401 gatcggcgcc tgctataccg cctaaattgc cgttaaagcc accaccactt gtcgccacga
     2461 aaatataaca aggcccttcg ttcttgcaac tgacaaaacc cgctggattt tccagcatat
     2521 cgaccaagcc atagttattg catccagtaa aaacaaaggt agttaatgaa aataccgcca
     2581 acgtatgcac aaatactcgg gggtgattat agcttttcat agccaaacgt agcgcagctt
     2641 tgatggcgtg tcatgctttt tttgagtatt tattcttcaa gtctgaattg ttgagaataa
     2701 caccggtaca gcaacgggcg gcgaacgact ggggaaggaa tccgctgtta aaccaacgag
     2761 ttccggcatg gcctccttat cgcggtagta ctggcattac acttttgcca attcagttgt
     2821 ttttagtgat aagctggcag gaataactat tctgattttc ggattatgtc acattagggg
     2881 ataactatcc tggttttcgg attatgtcgc attagagggt aactatcccc atttttcgga
     2941 ttatgtcaca ttagcaggct gttgaaaaaa acgcttaata ttccaaaatt ggggactgcc
     3001 tgaatt
//
LOCUS       DAOMPD010000332         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13310_length_3002_cov_1.835426, whole genome shotgun sequence.
ACCESSION   DAOMPD010000332 DAOMPD010000000
VERSION     DAOMPD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13310_length_3002_cov_1.835426"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLY93_10780"
     CDS             <1..501
                     /locus_tag="PLY93_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP70005.1"
                     /translation="DAGVKIYHPNYEVWDAALFKKICPGKERFIGRDNWIRRVVESAK
                     AFGAENVIPNFVGGVEMADTIGFTDIDKAVSSTSEGLDFFMSQGIIPRFTTWCPEPFT
                     PLGEISAPAPLLYYLKLLRNYQGIHKKYNLAVPPGYGEAGVGKAVFSVSAFMDVLEFL
                     GCLMGT"
     gene            complement(478..>1900)
                     /locus_tag="PLY93_10785"
     CDS             complement(478..>1900)
                     /locus_tag="PLY93_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70006.1"
                     /translation="TKTLERKAQIKTEYGTQNALGARILVTKKDEVGTYLIDYKREKY
                     DYEAFGKNTVANSAFSLDALKLLGQLNFTPSYNMLIRTEYREQARGLQANSFYSHENK
                     KLAILGVDNLIRPTDNQRVTAGVTASFAEGTATDATTASTQNAGMKKLMGSLEWQYIF
                     GERNALTLSTDLWYGENTDYLTSTPVYYRAGNAEARNVFPLARFLIGENQQALQIDAT
                     VGVKVFFAQSMQPVWGPRVAFDFFYPGYQSTLELERTGSVPDAEKYFFNVLYQSPYRF
                     LSAEEVWKAAFKNNFHLTKETHLKASIILLNYPLYFDRKIESATGLLTLTPILYRTVQ
                     GSLSLAQNFGAYFYHDTGLEAEYSIDVVSLREPFALFSRIHFTPPSWDFSLELKYVAA
                     RREIDATSLAKTTLSAYTLLGASIEYTLLPSLKFFIRGENLLNQKYEYVSLYRTSGAR
                     GWFGLNMVFQTLSHRRLRPHETT"
     assembly_gap    1901..1910
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1911..2187)
                     /locus_tag="PLY93_10790"
     CDS             complement(<1911..2187)
                     /locus_tag="PLY93_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70007.1"
                     /translation="MKIVPVLLSYAALMMGLFPFTAAPASPGLDIEDELNFNKDVRNK
                     KKSPSLNEDKAPEETITDKDTTIEGDDEQYAPPKKSDIELPDIAEGRL"
     assembly_gap    2454..2463
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2532..>3002)
                     /locus_tag="PLY93_10795"
     CDS             complement(2532..>3002)
                     /locus_tag="PLY93_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70008.1"
                     /translation="VGKVNTAYKALHDYFSEKENTAPSLQEVFDAVCAIRRSKLPNPR
                     EIGNAGSFFKNPIVDLEKYKALKEKYPTLPRFTVGINSIKIPAAWLIENLGYKGTTRA
                     DANGAKYGVHAKQPLVLVNYGGASGAAILSLAHEIQERVQEKFDIGLEMEVNII"
BASE COUNT          705 a          651 c          790 g          836 t
ORIGIN      
        1 gatgccgggg taaaaattta ccatcccaac tacgaagtgt gggatgccgc gcttttcaaa
       61 aaaatttgcc ccgggaaaga gaggttcatt ggccgcgaca attggatccg ccgtgtggtt
      121 gagtcggcaa aagcgtttgg cgcagaaaac gtaatcccca attttgtggg cggagtagag
      181 atggccgaca ctatcggctt taccgacatc gacaaggcgg tgtcctcaac ttcagaaggg
      241 ctcgattttt ttatgagcca agggattatc ccacgcttta ccacctggtg tccagagcct
      301 tttacgccgc tgggggaaat ttcagcgcct gcgccgctgt tgtattattt gaaactcttg
      361 cggaactatc aaggtattca caaaaaatat aacctcgccg ttccaccggg ctatggcgaa
      421 gcgggcgtgg ggaaagcggt ctttagcgtg agcgcgttta tggatgtact tgagttttta
      481 ggttgtctca tggggacgta agcgcctatg agacaacgtt tgaaatacca tgtttagacc
      541 aaaccaacca cgcgcgcccg aggtgcggta gagcgacacg tattcgtatt tctggttcaa
      601 gaggttttct ccacgaataa aaaatttcag cgaaggcaat agggtgtact cgatgcttgc
      661 tcccaagagc gtgtacgctg acagtgtggt ctttgctaaa cttgtcgcgt cgatttctcg
      721 gcgtgcagcc acatacttga gttcaagact aaaatcccac gagggtggag taaaatgaat
      781 gcgtgagaaa agcgcaaagg gctcgcgtag cgatacaacg tcgatggagt actcggcttc
      841 aagcccggtg tcgtggtaaa aatatgcacc aaaattttgc gccaaggaga ggctgccttg
      901 tacggtacgg tataaaatcg gggtaagggt gagtaggcct gtggcgctct ctattttacg
      961 gtcaaaataa agagggtagt tgaggagtat tattgacgct ttcaggtgtg tttctttggt
     1021 gaggtgaaaa ttatttttga acgcggcttt ccaaacttcc tcggcgctca aaaaacggta
     1081 gggggattga tagagcacat tgaaaaaata cttttcagca tcgggaacac tccccgttct
     1141 ttctaattcc agtgtgcttt ggtaacccgg atagaagaaa tcgaacgcga cgcgcggccc
     1201 ccaaaccggt tgcatacttt gtgcaaagaa aactttcacc cccaccgtgg cgtcgatttg
     1261 caacgcctgt tgattttctc caatgagaaa acgcgccagt gggaagacgt tgcgcgcctc
     1321 ggcatttccc gcacgataat ataccggtgt ggatgttaag tagtcggtgt tttcaccgta
     1381 ccacaaatcg gtcgagagtg tcagcgcgtt gcgttcgcca aagatatatt gccattcgag
     1441 cgagcccatg agttttttca tgcccgcgtt ttgggtgctt gcggtcgtcg cgtcggtggc
     1501 cgtcccttcg gcaaaagaag cggtgacgcc cgccgtgact cgttggttgt cggtggggcg
     1561 gatgaggtta tctactccca ggatggcgag ttttttattc tcgtgtgaat aaaaagagtt
     1621 cgcttgcaaa ccgcgcgcct gttcgcggta ttcggtgcgg atgagcatat tgtagctggg
     1681 ggtgaagttg agttgcccca aaagtttgag cgcatcgagt gaaaaagcgg agttggcaac
     1741 ggtgttctta ccgaacgctt cgtaatcgta tttttcgcgt ttgtagtcga tgaggtatgt
     1801 acccacttca tcttttttgg tgaccaaaat ccgcgcgccc aacgcgtttt gggtaccgta
     1861 ttcggtttta atttgcgcct tgcgctctaa agtctttgtg nnnnnnnnnn aaaggcggcc
     1921 ttcggcgatg tcggggagtt caatgtcgct ttttttgggt ggggcgtatt gttcatcgtc
     1981 gccttcgata gtggtgtctt tgtcggttat ggtttcttcg ggtgccttat cttcgttgag
     2041 agagggcgat ttctttttgt tacgaacgtc tttattgaaa ttcagttcgt cttcgatgtc
     2101 taacccgggg gatgcgggag cagccgtaaa ggggaataat cccatcatca acgcggcata
     2161 gcttaaaagc acaggtacaa ttttcatgga gtttgaaaga cttacttttc tcgacgcgct
     2221 gtaaacggta atgtgggaag agcctaattt gcctcagtga cttttaatcc gaccactaat
     2281 gtaggaaact gaatttgtaa ccctccaacc aaacgccgct caggctgctc tgctcgtatg
     2341 gcatagggta tttaatgtcc ttttggagtg tataagatgt cagggataac tatgccgatt
     2401 ttctggatta tgtcacattt agggataact atgcaccgca gacactggag tggnnnnnnn
     2461 nnncaaaatt aggggaaaat ttaaggaaca actcctttct atatgtacag gctattataa
     2521 ttcgcagcat ttcaaataat attgacttcc atctctaacc caatatcaaa tttttcctga
     2581 acgcgttctt ggatttcgtg cgccaacgat aaaattgccg cgcctgatgc accaccataa
     2641 tttacaagaa cgagcggttg cttggcgtgt acgccatact tggcgccgtt tgcgtcagcg
     2701 cgggtggtgc ctttgtagcc caaattctca atcagccatg cggcgggtat tttgatagaa
     2761 tttataccga cggtaaaacg aggcagtgta ggatattttt cctttagtgc cttgtacttt
     2821 tctaaatcca caatggggtt tttgaaaaaa ctaccggcat taccgatttc tcgtgggttg
     2881 gggagtttgg agcggcgtat cgcacaaact gcatcgaaga cttcttgtaa cgagggcgct
     2941 gtattttctt tttcggaaaa atagtcgtgc agcgctttgt acgccgtgtt gacttttcct
     3001 ac
//
LOCUS       DAOMPD010000333         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13323_length_2999_cov_2.571332, whole genome shotgun sequence.
ACCESSION   DAOMPD010000333 DAOMPD010000000
VERSION     DAOMPD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13323_length_2999_cov_2.571332"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..879
                     /locus_tag="PLY93_10800"
     CDS             79..879
                     /locus_tag="PLY93_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRP70009.1"
                     /translation="MVILRERRHQPLSFERYYGNFTPTLASHRGYGKHGPIAENTLPA
                     FMASEKLGFHAHEFDVRKTQDNQVMLLHGPRLEVTSDGVGRMEDTPSAQIQKLDAAHY
                     LLDKTDKRKKHTRVPFPTLEKVLTTLPKNSKLNIEIKRDRWDLSRGVEALTIQVVEKA
                     QSESRVAFSGFHFLTLWYLRRLKTKIPVGLLIEPGIFARAKLFLYRYLLLPDNIHLHY
                     STATPYLLAKLKKRGYGLAFWTVNDESIARYLFNHGADIVITDNMQLK"
     gene            complement(907..2055)
                     /locus_tag="PLY93_10805"
     CDS             complement(907..2055)
                     /locus_tag="PLY93_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRP70010.1"
                     /translation="MRIAFDAKRAFHNQTGLGHYSRSLIQAVATHFPQHEYFLCNPKP
                     SALFPLRENNLHEVLPTTFWAKKFSSLWRSGGIKKDLARLKIDLYHGLSHEIPRGIHK
                     TNIKSVVTIHDLIHERYPEQYSAIDVRVYRHKFRYACANADAIIAISEQTKRDIIDFY
                     NIPEKKIRVCYQSCNPIFEKNVSTKEKERVRRMYSLPPKYFLYVGSIIERKNLLNVLK
                     ALYLLKNKTSTPLVVIGNGKKYKNEVKNYLRENQLINRVIFLSENKAASTPEFISSAV
                     FPALYQMATAMVYPSSFEGFGIPVLEALWSRLPVITSNTSCLPEAGGPSSYYITPTSP
                     QEIATAMERIENDILLAKKMADAGLKYAANFTSAKSAAAVMQVYEDSI"
     gene            complement(2059..2565)
                     /locus_tag="PLY93_10810"
     CDS             complement(2059..2565)
                     /locus_tag="PLY93_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70011.1"
                     /translation="MIQFWRLGLKRAPMMWILAGALYLVAYVETAVFVRYAKHLSTNN
                     LVLLYAFLLLLIGFAFGVLMVQAILCRLLRYEIGNFETLGFTRTKIIGYYALQNIFVV
                     LLALLPAISTDLILSSLFAQKNVETASFDRVILSQFMLAMGITASYVIFLLLYTQKSP
                     ALLLKERL"
     gene            complement(2562..>2999)
                     /locus_tag="PLY93_10815"
     CDS             complement(2562..>2999)
                     /locus_tag="PLY93_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRP70012.1"
                     /translation="RTNLSKMRARGRAASGIIGMTLEGQDYMVGLDVVSGKNSHLLVV
                     SESGYGKRVKYDEFGAKGRGGKGMAFFKATEKTGKAVVIRTVTEDAEVIVTTNSGMVL
                     RSEVSQIAQQGRAASGVRVVDMTAGDKVADMTVYVPESATDKK"
BASE COUNT          771 a          702 c          712 g          814 t
ORIGIN      
        1 ttgacgcctg ttttgtaaaa aaaaataatg gctattattt ttataataat tgtagtactc
       61 actcttactt tgttattaat ggttattctc cgcgaaagac gccaccagcc gctttcgttt
      121 gaacgatact acggcaattt cacgcccacg cttgcctcgc atcgtggcta tggcaagcac
      181 ggccccatcg ctgaaaacac gctacctgca tttatggcgt ctgaaaaatt gggatttcat
      241 gcgcacgagt tcgatgtgcg taaaactcaa gacaaccaag tgatgctctt gcacgggccg
      301 cgtctcgagg tgacgagcga tggtgtgggt agaatggaag acactccttc ggcgcaaatt
      361 caaaagctcg atgccgcgca ctatctgtta gataaaaccg acaaacgaaa aaaacacacg
      421 cgtgtgcctt tccccactct ggaaaaagtg ctgacgacgc ttcccaaaaa ttcaaagctc
      481 aacattgaga ttaaacgcga tcggtgggat ttatcccgtg gggttgaagc gctgacgatc
      541 caagttgtgg aaaaggcgca aagtgaaagc cgtgtcgcat tttcgggatt tcattttttg
      601 acgctctggt atctgcgtcg tcttaaaaca aaaatccccg tggggcttct catcgagcct
      661 ggaatttttg cgcgggcaaa gcttttcctt tatcgttatt tattactccc cgacaatata
      721 cacctgcact attccactgc cacgccgtac cttttggcaa aactcaaaaa acgcggttat
      781 ggtttagcgt tttggacggt gaacgatgag agcatcgcgc gctatttatt taatcatggc
      841 gccgatatcg tcatcaccga taatatgcaa ctgaaataaa attccgttct attttgcgta
      901 gggcaactat attgagtctt cgtaaacctg catcactgcg gcggcgcttt tcgcgctcgt
      961 gaaatttgcc gcatacttaa gaccagcgtc ggccattttt tttgcaagca gaatgtcgtt
     1021 ttcaatgcgc tccatcgctg tggcaatttc ttggggtgaa gttggggtga tgtagtatga
     1081 actcgggccg cctgcttcgg ggaggcacga tgtgttcgag gtgatgacgg gaaggcggct
     1141 ccacagcgct tctaaaacag ggataccaaa accttcaaag gacgatggat acaccatcgc
     1201 tgtcgccatt tggtagagcg cagggaaaac cgccgacgag ataaactcgg gcgtggatgc
     1261 ggctttattt tctgagagaa aaatgacgcg gttgattaat tgattttcac gcaaataatt
     1321 tttcacctcg tttttgtatt ttttaccatt gccaatcaca acgagtggtg ttgatgtttt
     1381 atttttgagt aagtaaagcg ctttgagtac attgagtaaa tttttacgtt caataatcga
     1441 accgacgtac agaaaatatt ttggaggtaa cgagtacatc cgccgcacgc gttctttttc
     1501 ttttgtgctc acattttttt caaaaatggg attacaactt tggtaacaaa cgcgaatttt
     1561 tttttcgggt atgttataga aatcgataat atcgcgtttg gtttgctcgc tgatggcgat
     1621 aatcgcgtcg gcgtttgcgc acgcgtagcg aaatttatgg cggtacaccc tcacgtcgat
     1681 ggcgctatat tgttcggggt aacgctcgtg gattaaatcg tggatggtca ccaccgattt
     1741 tatatttgtt ttgtggatac cgcgcgggat ctcgtggctc aagccgtggt agaggtctat
     1801 tttcaagcgc gctaaatctt ttttgatgcc gcccgaacgc cacaaagagg aaaatttttt
     1861 tgcccaaaac gtggtgggta aaacttcgtg tagattattt tctctaagtg gaaacaaagc
     1921 gctgggtttg gggttacaaa gaaaatactc gtgttgtggg aagtgtgtgg ctactgcttg
     1981 gatgagacta cggctgtaat gccccaaccc cgtttggtta tggaaagcgc gcttcgcgtc
     2041 aaaagcaata cgcatcgctt aaagtctttc tttgagtaaa agtgcggggg atttttgagt
     2101 atagagcaaa agaaaaatga cgtaggaagc ggtaatcccc attgcgagca taaattgaga
     2161 gaggatgact cgatcgaacg atgccgtctc tacgttcttt tgcgcaaaaa ggctgctgag
     2221 aataaggtcg gtagaaattg cggggagtag cgcgagcaac acgacgaaaa tattttgtaa
     2281 agcatagtag cctatgattt tagtgcgggt aaatcccaac gtttcaaaat taccgatttc
     2341 atagcgcaac aaacggcata aaatagcctg caccatcagc acgccaaatg caaaaccgat
     2401 aagcagcagt aagaacgcat agagaagtac caagttattc gtgcttaaat gttttgcata
     2461 gcgcacgaag acagcggtct ccacgtacgc caccaaataa agcgcaccgg cgagaatcca
     2521 catcatcggt gcgcgcttaa gacccaatcg ccaaaactgt atcatttttt atccgtggcg
     2581 ctctcgggca catacaccgt catgtcggca actttgtcgc ccgccgtcat gtctaccacc
     2641 cgcacacccg atgcggcgcg gccttgttgt gcaatttgcg aaacttcgct gcgcaacacc
     2701 atacccgaat tggtcgtcac gatcacttca gcgtcttcgg tgacagtgcg gatgaccact
     2761 gccttgcctg ttttttcagt cgccttaaaa aacgccatcc ctttcccgcc gcgtcccttt
     2821 gcgccgaact catcgtattt gacgcgttta ccataccccg attcgctcac gacgagtaag
     2881 tgagaatttt taccgctgac aacatccaag cccaccatgt agtcttgccc ttcaagtgtc
     2941 atgccgataa tccccgacgc ggcgcggccg cgcgcccgca ttttactcaa gttggttct
//
LOCUS       DAOMPD010000334         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13363_length_2993_cov_2.488768, whole genome shotgun sequence.
ACCESSION   DAOMPD010000334 DAOMPD010000000
VERSION     DAOMPD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13363_length_2993_cov_2.488768"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLY93_10820"
     CDS             <1..320
                     /locus_tag="PLY93_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70013.1"
                     /translation="KESTKWLENKWAQIEGVSYEPAAIGQGVELETALRKLANTDAAK
                     KEAGKKELEAIIHEERSQFLEVARKILAEKTKPETETPQPQSESKSSESHDAHKGEEH
                     GNP"
     gene            307..1269
                     /locus_tag="PLY93_10825"
     CDS             307..1269
                     /locus_tag="PLY93_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRP70014.1"
                     /translation="MATRKQRKEPAIFIVNPQAGGGKSQRELKKLLDEIASLYGNAKI
                     EFTEEKGHATTLTRTALKNGVDLVVAVGGDGTISEVAAGFFDTKGKALLPLKEAPALG
                     ILPAGSGSDYARTLGIPRSSAIALQILQTAQTRTVDAGLLDFVDFQGKKTKRIFINIA
                     DIGIGGEVVEILERQGKKLGAFLSYQLATLRGLIAYKNKDLKIILDKKQELSAVYTGA
                     IVALGKYFGSGMQIAPNAEVDDGFFDVVLLGEMTKMELVAKMQKVRKGTHILDKNVAV
                     YRAKHVEISSTARALLDCDGELPGQTPVTFSIIPKAIKVVVPPR"
     gene            complement(1298..1750)
                     /locus_tag="PLY93_10830"
     CDS             complement(1298..1750)
                     /locus_tag="PLY93_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70015.1"
                     /translation="MFWLARAALSLLTLLILVGTVWLFWGDDLRRLGVEKKIKDTTKE
                     KINEKAKQTAKEAPKKIKKEIQTLKKDLSESTITIQAQDEWKKAQSGLSGIVAVERKN
                     NTALSVQLAQKNDERWHSILRRAPQNAPVAIPVRPPVKKESKDSVRRR"
     gene            complement(1794..>2993)
                     /locus_tag="PLY93_10835"
     CDS             complement(1794..>2993)
                     /locus_tag="PLY93_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70016.1"
                     /translation="SYVRENFRDFVGLSAGKLRIGGTKWEVGVFGLEGFADARHNFES
                     AGGYIGHPFVVDGQQHAFLFKARTVRGVLYDYYGGGIRQGFDPENGKFFDLIYRNRTE
                     EMSAEYLYGIGKNNRIEIGPAFEHRINQDYYINSSDQYTLQKTPALGVSKRAQDFYLN
                     EQFLTQGVSLNVNTRNGYYFPMKNFQRYLFTEDQFEGLRTRTKVLHANPIFGLSDHYT
                     LVDTYAVYQHNLFSDALRLETALARTATFWENRYATPRDDVWKVDVRFFNFHRWGTLA
                     ARTFTAVGNNMSANARREIAAQFTRGFYYGSFTPSSGFLASAEYRSPGVRLPYVLVAA
                     VAFIDYVGVGDATRHFDWYPIVGFGLRTMLYEIDNNVFRLDFGFNLNDSNLNLLKAIQ
                     FGMNHAF"
BASE COUNT          804 a          736 c          738 g          715 t
ORIGIN      
        1 caaaagaaag cacgaaatgg ctcgagaaca aatgggcgca aattgaaggc gtcagttacg
       61 agcccgctgc aatcggccaa ggtgtcgagt tagaaactgc gttacgtaaa ttagcaaata
      121 ccgacgctgc aaaaaaggaa gcgggcaaga aagaactcga agcaattatc catgaagaac
      181 gttcgcaatt cctcgaagtg gcgcgtaaaa ttctcgcaga gaaaactaaa ccagaaaccg
      241 aaacaccgca accacaaagc gagagtaaat cctccgaatc tcacgatgcc cacaaaggtg
      301 aagagcatgg caacccgtaa acagcgcaaa gagcctgcta tttttatcgt caatcctcag
      361 gcgggtggcg gaaaatcaca acgcgaatta aaaaagttac tcgatgaaat cgcttcgctt
      421 tacggtaacg caaaaattga attcaccgaa gaaaaaggcc atgccaccac actcacgcgc
      481 accgcgctaa aaaacggtgt cgaccttgtg gtggccgttg ggggcgatgg cacgattagc
      541 gaagtggcgg cgggtttttt tgacacaaaa ggcaaagcat tgttaccgct gaaagaagcg
      601 ccagcgctcg gtattttacc cgccggttcg ggaagcgatt atgcgcgcac gctgggcatc
      661 ccacgttctt cggcaattgc tctccaaatt ttacagaccg cacaaacgcg caccgtcgat
      721 gcagggctgt tggattttgt cgactttcaa ggtaagaaaa ccaaacggat ttttatcaat
      781 attgccgaca tcggtatcgg cggcgaggtt gtggaaattt tagagcgcca aggtaaaaaa
      841 ctgggggcat ttctctcgta tcaactcgcc acgttgcggg ggttgattgc atacaaaaat
      901 aaagacttga aaattatact cgataaaaaa caagaactga gcgccgtcta caccggtgcg
      961 attgttgcac tgggtaaata ttttggtagc ggcatgcaga ttgcgcccaa cgccgaggtc
     1021 gatgacggct tttttgacgt ggtgcttttg ggcgaaatga caaaaatgga actcgtcgct
     1081 aaaatgcaaa aagtgcgcaa gggcacgcat atcctcgaca agaatgtcgc ggtctaccgc
     1141 gccaagcacg ttgaaattag ttcgacagca cgcgcgcttt tggattgcga cggtgagcta
     1201 cctggacaaa caccggtgac gttttcaata atccccaaag cgattaaagt agtcgtgccg
     1261 ccacggtaga ttcaaactta aacggcggtt ataggcatta ccgccgtcgc accgagtctt
     1321 tggattcttt ctttacgggt ggtcgaacgg gtatggcaac gggcgcgttc tgtggagcgc
     1381 ggcgtaaaat gctgtgccaa cgctcgtcat ttttttgagc taactgaaca gagagcgctg
     1441 tgttgttttt gcgttcaaca gcaacaattc ccgaaagacc gctctgtgct tttttccatt
     1501 cgtcttgcgc ttgaatagtg atggtagatt cactcaaatc ttttttgagc gtttggattt
     1561 ctttttttat ttttttgggg gcctctttag cggtttgttt tgctttctcg tttatttttt
     1621 ctttcgtggt atctttgatt tttttttcca cacccaagcg ccgtagatcg tctccccaaa
     1681 aaagccaaac ggtgccgact aaaataagca gcgttaaaag agaaagtgcc gcgcgcgcca
     1741 accaaaacat tttctgcgtt tgggggtagc gagacacacc gccaagcaca tctttaaaaa
     1801 gcgtggttca tcccaaattg gattgcttta agtaagttca aattactgtc gttgagatta
     1861 aacccaaaat cgagacgaaa cacgttgttg tcgatttcat aaagcattgt gcgcaagccg
     1921 aaccccacaa tggggtacca atcgaagtgg cgcgtagcat cccccacgcc gacgtaatcg
     1981 ataaacgcca cagccgccac gaggacgtac ggcaagcgca cacccggcga gcggtactcg
     2041 gcggaggcca gaaatcccga actgggagtg aatgatccat agtaaaaccc acgcgtgaat
     2101 tgtgcagcga tttctcggcg tgcgttggcg ctcatgttgt ttcccactgc cgtaaacgtg
     2161 cgcgccgcca acgtacccca gcggtgaaaa ttaaagaaac gcacgtcgac tttccacacg
     2221 tcgtcgcgcg gtgtggcata tctgttttcc caaaatgttg ccgtgcgcgc caacgcggtt
     2281 tccaaacgca gcgcatcgct gaacagattg tgttggtaca ccgcgtatgt gtcgacaaga
     2341 gtgtaatgat cggataatcc aaagataggg tttgcgtgca acacctttgt tcgagtgcgc
     2401 aatccttcga actggtcttc ggtgaataaa taccgttgaa agtttttcat gggaaaataa
     2461 tacccgttgc gcgtgttgac gttgagcgac accccttgcg ttaaaaactg ctcgttcaaa
     2521 taaaaatctt gcgcacgctt agacacccca agcgcgggcg tcttttgcag ggtgtactga
     2581 tcgctgctgt tgatataata atcttggttg atgcggtgct cgaacgccgg accaatttca
     2641 atcctgttat tttttccgat gccataaaga tattccgccg acatctcttc ggtacggttg
     2701 cggtaaatta aatcaaagaa ttttccgttt tcgggatcaa agccttggcg gatgccgccg
     2761 ccataataat cgtaaagcac accgcgcacc gtacgcgctt tgaacaggaa cgcgtgctgc
     2821 tgtccgtcga ccacaaatgg atgaccgata taaccacccg cagattcaaa attgtgccgc
     2881 gcgtcggcaa aaccttcgag accaaaaaca ccgacctccc attttgtgcc gccgatgcgt
     2941 agtttgcccg cgctcaatcc cacaaagtcg cggaaattct cgcgtacata cga
//
LOCUS       DAOMPD010000335         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13405_length_2985_cov_2.912287, whole genome shotgun sequence.
ACCESSION   DAOMPD010000335 DAOMPD010000000
VERSION     DAOMPD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13405_length_2985_cov_2.912287"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLY93_10840"
     CDS             <1..323
                     /locus_tag="PLY93_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP70017.1"
                     /translation="LELNGYSVRRGGDIHEALEKFSSFSPGIVLIDASLPAKSWLELI
                     KCIRDYEVEKDSDYRCVILVTTTRYSNEIYQKAIKFGASDVVGKPVEHKVLLQSIVKY
                     ATQY"
     gene            331..1359
                     /locus_tag="PLY93_10845"
     CDS             331..1359
                     /locus_tag="PLY93_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70018.1"
                     /translation="MALRFFTAALLTGSLGAAKVKPKKEIPQANGVDPISVYIQHGLP
                     GVAHDTKEKRFYWLYGIDAYYSYGSMVINFTDKPMQDLGEDSELKIYENLLVSPFSPP
                     RYALLEASVYPLPIGGAVVRSEAYDFYQRAQIGGEGFNLIRSATRGYQEPYAVSLFLG
                     NMARYRPLARTPEEREINQQSGYQSSIGYTGYLISYGSHHIKDNVLFQDHWVEIGWKI
                     KGDQFFTLQTFQWDYGLGLKIHEHPDVSNIAFLSIKRNRLDYSAGAWDFRANSGFELR
                     VDFKLNSLVPVRVYFEVNKKWPIKKATAFILVLGFTWESDSLYEGVLNTYSGGQNFQV
                     LLKPNITF"
     gene            1373..2059
                     /locus_tag="PLY93_10850"
     CDS             1373..2059
                     /locus_tag="PLY93_10850"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMUG2 DNA glycosylase family protein"
                     /protein_id="HRP70019.1"
                     /translation="MKKTLADKIIAFNNSLHFSGTLPKGFQVLNPFRDNKETIPSMEK
                     FYRKFYNDTTPRRFIIGINPSRHGAGVTGVPFTDTKRLESECGITMHSVKTHEVSSVF
                     FYEMITAFGGVEKFYSQFYINSPFALAIVRETKPGVFLNANYYDDNALFLCVKDYMIE
                     TLKRHTAVGLITKEVFVLGKKNAGFIQKLNNESSFFDKITVLEHPRYIQQYKSKEKQY
                     YIDKYLAAFA"
     gene            2142..2774
                     /locus_tag="PLY93_10855"
     CDS             2142..2774
                     /locus_tag="PLY93_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRP70020.1"
                     /translation="MDQIFKKIALVGDVWVLWILLLASIYSVAVMIDRFRVFHKNKLN
                     FESFFSALKSLLEKRDTEGALGLCNAHAALEARVTLAGLSHFSKGATSVQEAMQSALV
                     LERAHFEKNLIVLATMGNNAPFVGLLGTVLGVIKAFHDLGVAGQSGVSVVMSGISVAL
                     IATAFGILLAIPAVVANNYFQTRLKRRVQNTQAIMHFMQVHLNAQGENFP"
     gene            2774..>2985
                     /locus_tag="PLY93_10860"
     CDS             2774..>2985
                     /locus_tag="PLY93_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRP70021.1"
                     /translation="MNDDTDDGIVSGINVTPLVDITLVLLIIFMVTAHFVTDEGLKVH
                     LPKAVAAEATPTPAVTVTSSKEGEIRL"
BASE COUNT          823 a          619 c          714 g          829 t
ORIGIN      
        1 ttttagaact gaatggttat tcggtgcggc gtggtggcga tatccacgag gcgttagaaa
       61 aatttagcag tttttcaccg gggattgtac tcatcgatgc cagcttaccc gccaagagct
      121 ggcttgaact cattaagtgt atccgcgact acgaagttga aaaagatagt gattatcgct
      181 gtgttatttt ggtcaccacc actcggtact cgaacgaaat ataccaaaaa gcgataaaat
      241 tcggcgcgag cgatgttgtg ggtaagcccg ttgagcataa ggtattgctg caatcgattg
      301 taaaatacgc gacacagtat tagcatgagc atggcgttgc gcttttttac cgccgcgcta
      361 ttgaccggta gtcttggggc ggcgaaagtt aaaccgaaga aagagattcc tcaagcaaat
      421 ggggtcgacc ccatttctgt ttatattcaa catggcttgc caggagtggc tcacgacaca
      481 aaagaaaagc ggttttattg gctctatggt atcgatgcgt attattcata cggtagcatg
      541 gttattaact ttaccgataa accgatgcaa gacttgggcg aggatagcga actgaaaatt
      601 tatgaaaatt tattggtgtc gccgttttca ccgccgcgat atgcgctttt agaagcaagc
      661 gtgtatccgc tgccgatagg cggtgcggtt gtgcgcagcg aagcgtacga cttttaccag
      721 cgggcacaaa ttggcggcga aggatttaac cttattcgct ccgcgacacg aggctaccaa
      781 gagccgtacg cggtgtcgct atttttaggt aacatggcac ggtaccgccc attggcgcgc
      841 acacccgagg agcgtgaaat taaccaacaa tcgggctacc aaagttcgat tggttatacc
      901 ggttacttaa tcagttatgg ttcacaccac atcaaagata acgtgctctt tcaagaccat
      961 tgggtcgaga ttggatggaa aattaaagga gatcaatttt tcaccttgca aacttttcag
     1021 tgggattatg gacttggttt aaagatacac gagcaccctg atgtgtcgaa tattgcattc
     1081 ttaagtatta aacgaaatcg cttggattat tcggcgggtg cgtgggattt ccgtgctaat
     1141 agtggctttg agttgcgtgt cgactttaag ctgaattcgt tggtgcctgt gcgggtttat
     1201 tttgaggtga acaaaaaatg gccaattaaa aaagcgacgg cgtttattct tgtattgggc
     1261 tttacttggg agtcggattc tttatacgaa ggcgttttga atacctattc aggcgggcaa
     1321 aactttcaag tgctcttaaa accgaatatt acattttagg atggtgtagt taatgaaaaa
     1381 aacactcgcg gataaaatta ttgctttcaa taactctctt catttttcag ggacgttacc
     1441 aaaaggattt caagtgctca atccttttag agataataaa gagacaatcc cctcgatgga
     1501 aaaattttat cggaaatttt acaacgacac cacgccgcgt cgtttcatta ttggtatcaa
     1561 tcccagccgc catggcgccg gagtcaccgg cgtacctttc acagacacta aacggttgga
     1621 gtctgaatgc ggtattacaa tgcattcggt gaaaacgcac gaagtgtcgt cggtattttt
     1681 ttatgaaatg attaccgctt ttggcggcgt agaaaaattt tatagccagt tttatatcaa
     1741 ttcacctttc gctttagcca ttgtacgcga aacaaaaccg ggcgttttct taaacgctaa
     1801 ctactatgac gacaacgcac tttttctttg tgtgaaggat tacatgattg aaactctcaa
     1861 gcgtcacacc gcggtgggtc tcattacaaa agaggtgttt gttctgggta aaaaaaatgc
     1921 cggttttatc caaaaactta acaatgaatc ctcttttttt gataagatca cagttttaga
     1981 acacccgcgc tatatccaac agtataaatc gaaagaaaag cagtattata tcgacaagta
     2041 tttagcggcg tttgcttaaa gtcataaaat ataaaagaga ttgacgcgct gttaaacttt
     2101 aatgttgaga atgattatca cttgctataa ataagagaac aatggatcaa attttcaaaa
     2161 aaattgcact cgttggggat gtctgggtac tctggatttt actcttggcg agtatttatt
     2221 cggtggcggt gatgatcgac cgctttcgtg ttttccataa aaacaaactc aactttgaaa
     2281 gtttttttag cgcgttaaaa tcacttttag aaaaacgcga cacggaaggc gcgcttggac
     2341 tctgtaatgc ccacgcggcg ttagaggcgc gggtcactct ggctggactt tcgcacttta
     2401 gcaaaggcgc aacttcagtc caagaagcga tgcagtcggc gctggtttta gaacgtgcgc
     2461 acttcgaaaa aaatctcatc gtactcgcga ccatgggaaa taatgctcct ttcgttggtc
     2521 ttttaggaac ggtcttagga gtcatcaaag cgttccacga cttgggcgtc gccggccaat
     2581 cgggggtgtc ggtggtgatg tcggggattt cagtggcgct catcgcgacg gcattcggta
     2641 ttcttttagc aatcccagcc gtggtcgcaa acaactattt ccaaacgcga ctcaaacggc
     2701 gcgtacaaaa tacacaggcg attatgcatt tcatgcaagt gcacctgaac gcacaaggag
     2761 aaaatttccc gtgatgaacg acgataccga cgatggcatt gtgagtggaa ttaatgtgac
     2821 accgcttgtg gacatcacat tggtgcttct tatcatcttt atggttactg cgcactttgt
     2881 caccgacgaa ggtctcaaag tacatttacc caaagcggtg gcggcagagg cgacgccgac
     2941 gccagcagtc acggtgactt cttccaaaga aggggaaatc cgact
//
LOCUS       DAOMPD010000336         2972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13470_length_2972_cov_2.312993, whole genome shotgun sequence.
ACCESSION   DAOMPD010000336 DAOMPD010000000
VERSION     DAOMPD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13470_length_2972_cov_2.312993"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PLY93_10865"
     CDS             complement(<1..1391)
                     /locus_tag="PLY93_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRP70022.1"
                     /translation="MKIFFCILFSLPLLHCTPSTPPQPTAKQGVLSLQADAASNRQAW
                     DFKRDGTLTLDGEWELYWNQLLDPIDFNHNTNPKPTAIVRIPDVWKEQNINGVQTSNF
                     GYATYRLRIRFPEKSFLQRQLLALRVHYELTAYRVFYNGIAVATNGNVTTDASHFQAE
                     YRPLTVNLPTEDTDDVEIIVQVANFVHQSGGFITPIKLGLASTLIADTRLKQLTDAFL
                     LGATLLMALYFIMLFLWRPVDKAPLWFALSCFAIITRICVTSERLIFVVIPNFPWDML
                     LRTTYVSTLVALIAAIFYVKNLLPLDTNRWVIRIITLVSVLLAIPILFLSPLQFTHIV
                     FGVEVITIAALSYMVFVSFLAFVRKRENTFPLFLGFIFIVIAAMNDILYNHLQVGIGY
                     IIPYGVLALILMHSASLLIRLATSFRRAELRKEDLEAEVALRTKRLSEAVHVAENAIQ
                     EKSVFFAMMTHEL"
     gene            complement(1388..1711)
                     /locus_tag="PLY93_10870"
     CDS             complement(1388..1711)
                     /locus_tag="PLY93_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70023.1"
                     /translation="MALWVGFHSFNLKENPAVLTSFGFQLMLYFGLFTVFSVRERRRF
                     WSSRPSRVMLLAGFFEAALGALVSIVGLPYFAAIPWTLTFFSISSTATVVFIFNDWVK
                     ARLYR"
     assembly_gap    1822..1852
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1853..>2972)
                     /locus_tag="PLY93_10875"
     CDS             complement(<1853..>2972)
                     /locus_tag="PLY93_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRP70024.1"
                     /translation="VLVTRASALEDAASMAVLCADKTGTLTQNKLHVHEIFPANDFNA
                     DEVLQYAALASKEENHDPIDIAILTRVRESHLSLPAAHVQKFIPFDPTRRHTAATLVL
                     SGATTTVYKGAVDTILSITPLTEDAAQSVAYTVEDQAAHGFRSLAVALQRENASPRFV
                     GFLTLFDAPRSDAAGLITQLAEEGVAVKMLTGDALPIATELGEALQLTSIISVKDPSL
                     QTEDANGSEKIQHPAIERLVKNPHIGGLAEVFPADKFNVVKAYQEAGYIVGMTGDGVN
                     DAPALRQAEVGIAVRNATDMAKGAASVVLTTDGLESILPLVREGRMVSQRILTWIVNK
                     VSLTILKTAFVVFGFLLTGQFVIETLGIILVALISDFTK"
BASE COUNT          827 a          686 c          731 g          697 t
ORIGIN      
        1 cttaactcgt gcgtcatcat tgcaaaaaat accgattttt cttgtatcgc attttcagcg
       61 acatgcaccg cttcgctcaa tctttttgtg cggagagcaa cctcagcttc aaggtcttct
      121 ttgcgcaact cagctctgcg gaaactcgtg gcaaggcgaa tcaaaagcga ggcagagtgc
      181 atcagaataa gagccagtac cccataaggg atgatgtacc caatacctac ttgaagatga
      241 ttgtagagaa tatcattcat agcagcgatg acaataaaaa taaatcctaa aaaaagaggg
      301 aatgtattct cgcgcttacg tacaaacgct aaaaatgaaa cgaacaccat gtaacttaga
      361 gcagcgatgg tgataacctc aaccccaaaa acgatatgcg taaattgtaa aggcgataaa
      421 aaaagaatgg gtattgccaa aagtacgcta acaagcgtta ttatgcgaat aacccaacgg
      481 tttgtatcca acggcaaaag attttttaca taaaagatcg ctgcaatgag tgcaacaagc
      541 gttgaaacgt acgtcgtgcg taacaacata tcccatggaa agttgggtat gacgacgaaa
      601 ataagtcgtt cgcttgtgac gcatatacgc gtaatgatgg caaaacaact caatgcaaac
      661 caaagtggcg ctttgtcaac aggccgccag agaaacagca tgataaaata aagcgccatg
      721 agcaacgtgg cgccgagtaa aaatgcgtcg gtgagttgtt ttagtcgtgt gtccgcaatc
      781 agagtggatg ccaagccgag ttttatggga gtaataaacc cacccgattg gtgcacaaaa
      841 ttggcaactt gtacaatgat ttcaacgtcg tcggtatctt ctgtgggtaa atttaccgtg
      901 aggggtcggt attcggcttg aaagtgcgag gcgtcggtag tcacattgcc gttcgttgcc
      961 acggcaattc cgttataaaa gactcgatac gcggtgagtt cataatgaac gcgcaacgcc
     1021 aacaactgcc gctgtaaaaa acttttttct ggaaagcgaa tacgcaagcg atacgtggca
     1081 tagccaaagt tcgatgtttg cacaccgttg atgttctgtt ctttccacac gtcggggatg
     1141 cgcacgatgg cagtaggctt ggggtttgtg ttgtgattga agtcgatggg atctaaaagt
     1201 tgattccagt agagttccca ttcaccgtca agagtcaatg tgccgtctcg tttgaaatcc
     1261 cacgcttgtc ggtttgaggc tgcgtcggct tgcaacgaca agacaccttg ttttgccgtc
     1321 ggttgtggcg gtgtacttgg agtgcagtgt aataatggta gagagaatag gatgcaaaaa
     1381 aagattttca tcgataaagt cgtgctttga cccaatcatt gaatataaaa acgactgttg
     1441 ctgtcgagct aattgaaaaa aatgtcagcg tccacgggat ggcggcgaaa taaggcaaac
     1501 caacgatgga taccagagcc cccagagcgg cttcaaaaaa accagccaga agcatcactc
     1561 ggctgggacg ggacgaccaa aaacgccgcc gctcgcgcac cgaaaaaaca gtgaacaaac
     1621 caaaataaag catgagttga aaaccaaacg atgtcagtac ggcaggattt tcttttaaat
     1681 taaaagaatg aaatcccacc caaagcgcca tgagcgattc tatagttaat aatactccaa
     1741 gcgtcgcgcc aactttaatc ggtattttta agttccaagt ttctactgtg tgcggtgcgt
     1801 gcgagcggtc tgtctagagc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttagtaa
     1861 aatccgaaat gagcgcaacg aggatgatcc ccaaagtttc gatgacgaat tgaccggtga
     1921 gcaaaaatcc aaaaacgacg aatgccgttt ttagaatagt gagactcact ttgttcacaa
     1981 tccaagtgag gatgcgctgc gacaccatac gcccttcgcg caccaacggc aaaatgcttt
     2041 caagaccgtc ggtcgtcagc acaacgctcg ccgctccttt ggccatgtcg gtggcgttgc
     2101 gcactgcaat acccacctcg gcttggcgca gcgcaggagc atcgttcacg ccgtcgcctg
     2161 tcataccgac gatgtagccc gcctcttggt acgcttttac cacgttgaat ttgtcggcgg
     2221 gaaaaacttc agccaagcca ccgatgtgtg gattttttac gaggcgctca atcgcggggt
     2281 gttggatttt ctctgaaccg tttgcatcct ccgtttgcag cgatggatcc tttacagaaa
     2341 taatcgacgt gagttggagc gcttccccca actctgtagc aatgggcagc gcatcgccgg
     2401 tgagcatttt taccgccacg ccttcttcgg caagctgtgt aattaaacca gcggcatcgc
     2461 tacggggcgc atcgaagagc gtgagaaatc ccacaaagcg tggggaggca ttttcacgct
     2521 gcaacgccac cgccaaagaa cgaaacccat gcgcggcttg gtcttcgacg gtatacgcaa
     2581 cagattgcgc ggcatcttct gttaaaggcg taatgctaag gatggtatcg acggcgcctt
     2641 tataaaccgt tgtcgttgct ccacttaaaa caagcgttgc tgcggtgtgc cgccgcgtag
     2701 gatcaaacgg gataaatttt tgaacgtgtg ccgccggtag agaaagatga ctttcacgta
     2761 cacgggtcag gatggcaatg tcaatcggat cgtggttttc ttctttagaa gcgagcgcgg
     2821 cgtattgcaa aacttcatcc gcgttaaaat cgttcgcagg aaaaatttca tggacgtgga
     2881 gtttattctg agtgagcgta ccggttttat ctgcgcacaa caccgccatc gacgcggcgt
     2941 cctcaagcgc gctggcgcgt gtcaccaaca cc
//
LOCUS       DAOMPD010000337         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13497_length_2967_cov_2.723901, whole genome shotgun sequence.
ACCESSION   DAOMPD010000337 DAOMPD010000000
VERSION     DAOMPD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13497_length_2967_cov_2.723901"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1012)
                     /locus_tag="PLY93_10880"
     CDS             complement(<1..>1012)
                     /locus_tag="PLY93_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRP70025.1"
                     /translation="NDVVAAYLTSSAGMISGVFALSYADNRLSIKTENTKVSLPAYSS
                     TAFNYSGTTILVLPDAIIREGTGKLSVDTFVFEYKNLSYNPHEGIFFNFTAQTPLTSQ
                     KIIPLPNALTGFLYAEGQYKKKIPSGVLQIKNFSFKESALSLDADTLAMAFEGSMLFV
                     KKQRVRLNKKWPIENLSLRLGATSSTLSVENLSMVLARGTINATYILNRQTKVQNLKL
                     SASHIKAQDLSALFSLKTTVFGNVTAAGQLSFAGFNAESIGKSLSGNLSLQIGRGKIK
                     DSFFQKGIFTGPLYKLEQKFSDIEFASGTADVLFSQGNIHARKVFFDAEEWNVKLRAE
                     ANR"
     assembly_gap    1013..1022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1023..1761)
                     /locus_tag="PLY93_10885"
     CDS             complement(<1023..1761)
                     /locus_tag="PLY93_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70026.1"
                     /translation="MFKWLLRVLIGLLLLLLLVSTVFFFYIRHSYSPEKIKPALVALL
                     QEKSGFGVSIKTLDFTWSGDIRLEKVCFRNMEMQLSRCFASAEKIFLDLKISALLKKQ
                     IVVNAVDVRGAALEFFSEKSSQKNKKNQFVYSWQNVRPTHPLSAETQNSETKFFSLSD
                     LHLNTIKVTDAKIIQDVQALGLPLGEISFSFVYRARNNHAFKLELTLPQKGKAVFDAQ
                     LRFADFPSTIQMIVESFRLNASDRIVGT"
     gene            complement(1819..2106)
                     /locus_tag="PLY93_10890"
     CDS             complement(1819..2106)
                     /locus_tag="PLY93_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRP70027.1"
                     /translation="MKKLFFLLLTVTAVFAEAEPARVKLPLLDLEHVLAVLVYSVIGI
                     ITMVLTFVIIDWIHPRDIWGEIADKQNHAMAIIFGCMILGIAIIIAASVVG"
     gene            complement(2154..2354)
                     /locus_tag="PLY93_10895"
     CDS             complement(2154..2354)
                     /locus_tag="PLY93_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70028.1"
                     /translation="MKEILKMYIFIPASLFLAGLSVYQIWTKSSGQEPIEAKNLDIKS
                     IRANPTSFRAVFLPRILAGGGK"
     gene            complement(2400..>2967)
                     /locus_tag="PLY93_10900"
     CDS             complement(2400..>2967)
                     /locus_tag="PLY93_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70029.1"
                     /translation="VTGKGFIYESQLSHYWGGSGEDSWSEGSNSTNFKTQAIPAGKYS
                     VFLMGATNIGVLQFKQLLSRFGSRVVLNIATRPSTPLAGGGALQNANPTQKPTMNQAT
                     NPLGFVAGDAKTKTGSLLPLAFPGKEEAPKLNLRMLAVRDVLDTGQNFWLFLFLFLGV
                     GYYHIRYKMKEKKRLADDGDDSDDDDDE"
BASE COUNT          865 a          651 c          604 g          837 t
ORIGIN      
        1 ctcgattggc ctcggcgcgt agttttacat tccactcctc tgcgtcaaaa aagactttgc
       61 gtgcgtggat atttccttgc gaaaacaata catccgccgt acctgaggcg aattcaatat
      121 ctgaaaattt ttgctccaac ttgtaaagcg ggcctgtaaa aattcctttt tgaaaaaaac
      181 tatctttgat tttgccacgg ccaatttgta acgataaatt ccccgaaagc gatttgccga
      241 tactttctgc gttaaatcca gcgaacgaca attgacctgc tgcggtcaca ttgccaaaga
      301 cggttgtttt cagagaaaag agagcgctta aatcttgagc ctttatgtgt gaagcggata
      361 attttaaatt ctgtactttt gtttgtcgat tgagtatata cgttgcattt atagtccccc
      421 gcgccaacac catactcaag ttttctacag ataaagtaga ggaagtagcg ccaagccgta
      481 acgacaaatt ttcaatcggc cattttttat tgagtcgaac gcgttgtttt ttcacaaaaa
      541 gcatcgagcc ctcaaacgcc attgcaagcg tatctgcatc gagagataaa gcgctttctt
      601 taaaagaaaa atttttaatc tgtaatacgc ctgaggggat cttttttttg tactgtccct
      661 ctgcgtataa aaatccggtg agagcattcg gcagtgggat aattttttga gaggtgagcg
      721 gtgtttgtgc ggtaaagtta aagaaaatgc cttcgtgagg gttatacgat aaatttttgt
      781 actcaaagac aaacgtatct acggataatt ttcctgtccc ttcacggatg atggcgtcgg
      841 gcaaaacaag aattgtggtt cctgaataat taaaagcggt agaagaatat gcaggcaaag
      901 atactttagt attctcagtt ttaatactga gtcgattgtc cgcataactt aaagcaaaaa
      961 ctcctgaaat catccctgca gaagacgtga gatatgccgc aaccacatcg ttnnnnnnnn
     1021 nngtgtaccc acgatacgat cgcttgcgtt caaccgaaaa ctctcgacaa tcatttggat
     1081 cgtagaggga aaatctgcaa agcgtaactg cgcatcgaag acagcttttc ctttttgcgg
     1141 cagcgttaat tcgagtttga acgcatggtt gttacgtgcg cggtacacaa atgagaaaga
     1201 tatttctccc aacggcaacc ccaacgcctg cacatcttgg attatctttg catcggtaac
     1261 ttttatcgta ttgagatgta aatccgacaa cgaaaaaaat ttggtttcag aattttgagt
     1321 ttctgcgctg agcggatgcg ttggtcttac gttttgccaa ctatagacga attgattttt
     1381 tttatttttt tgcgaacttt tttcactaaa aaattcaagc gctgcgccgc gcacatcgac
     1441 tgcgttgacg acgatttgct tttttaatag tgcagatatt tttaaatcta agaaaatttt
     1501 ctctgccgaa gcaaagcaac gcgataattg catttccata ttccgaaagc aaactttttc
     1561 aagacggata tcccccgacc acgtaaagtc gagagttttt atcgaaacgc caaatccact
     1621 cttttcttga agcaaagcga cgagggctgg tttaattttt tcgggagaat aactatgccg
     1681 aatataaaag aaaaaaactg tacttactaa gagtagaagg agcagaagac cgattagaac
     1741 acgcagaagc catttgaaca tgatacctta ttattttgcg cgagccacac gcaaaataat
     1801 aaatataaac ctttaatctt aaccgacgac agacgcggcg ataataatgg cgatgcctaa
     1861 aatcatgcaa ccaaaaataa tcgccatggc atggttttgc ttatcggcaa tttcgcccca
     1921 aatatcgcgc ggatggatcc agtcgatgat tacaaaagta agcaccatcg taataatacc
     1981 gatgactgaa tacacaagga ctgcaagaac atgctctaag tcgagcaagg gtaattttac
     2041 ccgcgcaggc tcagcctcag caaacaccgc tgttaccgtc agcaacaaaa agaatagttt
     2101 tttcatatac ccctctttat aataaatttt ctaataagta cacactgcgt gcgttatttt
     2161 cctccgccag caagaatccg cggtaaaaaa acagcacgga aagatgtggg atttgctcga
     2221 atacttttga tatccaaatt ttttgcctcg atgggctctt gcccacttga tttagtccaa
     2281 atctggtaaa cagacaaccc cgctaaaaaa agcgaagcgg gtatgaaaat atacattttg
     2341 agaatttctt tcattctatg cacccttttt gttcctttcc tctcgcgcct ctaacgtcgc
     2401 tattcatcat catcatcatc tgaatcatcg ccgtcgtcag ccaagcgttt tttctctttc
     2461 attttatagc gaatgtggta gtaaccaacg cctaaaaata agaaaaggaa aagccaaaaa
     2521 ttttgtcctg tgtcgagcac atcgcgaacc gccagcatcc gcaagttaag cttgggggct
     2581 tcctctttgc ctggaaacgc aagaggtaac aaagagcctg ttttagtctt tgcgtcgcct
     2641 gccacaaaac ccagcggatt ggtggcctga ttcatcgttg gcttttgtgt tgggtttgca
     2701 ttttgcaatg ccccccctcc cgctaagggt gtcgaaggac gagtagcgat gttcagaaca
     2761 acccgcgagc caaagcggga tagaagttgt ttaaactgaa gcacgcctat attcgtcgct
     2821 cccatcaaga aaacagagta cttgccggca gggattgctt gggttttaaa attggtcgaa
     2881 ttcgaacctt cactccatga atcctcaccc gagcctcccc aataatgaga gagctgcgat
     2941 tcatagatga accctttgcc ggtgact
//
LOCUS       DAOMPD010000338         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13505_length_2966_cov_2.587771, whole genome shotgun sequence.
ACCESSION   DAOMPD010000338 DAOMPD010000000
VERSION     DAOMPD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13505_length_2966_cov_2.587771"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLY93_10905"
     CDS             <1..445
                     /locus_tag="PLY93_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP70030.1"
                     /translation="LILDEPISALDVSIQAQILNLLVDLRQQYGFAAIFITHDLSIVR
                     WFSDRVAVLYLGRVVESALADIIFEKSRHPYTKALSLSKLDTNVHKKEFFSLQGEIPS
                     PINVPSGCPFAPRCDRVVPQCTASFPPITKTGKDVFFHCYNPLEV"
     gene            446..1066
                     /gene="upp"
                     /locus_tag="PLY93_10910"
     CDS             446..1066
                     /gene="upp"
                     /locus_tag="PLY93_10910"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRP70031.1"
                     /translation="MSVKIFSEHALLQTKIATLRNVNTPHPEFVRTMQDVSTILCALV
                     SAEITTTKMSVKTPLTRTHGVKVEYHVVLVPILRAGLGMLEGFQSLLPTASVGHVGVE
                     RDHKTLKPKFYYYKVPTLRGAEVWVIDPMLATGGSLDFALTKIKDAKPRIIRAVSVLA
                     SPEGIAFVQKNHPDVHFYTAVLDTKLNNKGYIVPGLGDAGDRYFNT"
     gene            1066..1683
                     /locus_tag="PLY93_10915"
     CDS             1066..1683
                     /locus_tag="PLY93_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70032.1"
                     /translation="MYKAITRQASLNRLLLEHVVPRFERVKKIRFLCAKKTIHLRGII
                     REGDERIFGMKTGDIYFDFRMLKEEVGAQSVKMRLSKTKLMSSARPDMIKWSNRFSNK
                     IEDILLYHFTRGDSPFFMVNRKRRFIGFDLNFILMQIPPELDVLGKINIVNVSFEPRR
                     IVWYLNSNLVLQSVVQMFKPEYIEIEKIEAEADAQRLLTDINLIE"
     gene            complement(1685..2365)
                     /locus_tag="PLY93_10920"
     CDS             complement(1685..2365)
                     /locus_tag="PLY93_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70033.1"
                     /translation="MLPLTFAKNWYGLASDATNAFRLVTDFNLKEFAPDRNSLHHVNA
                     RMTSVHPTKDKKKYLEANFANDLEHVNDTLAIEKRAALTPTYASAEYRWNVMHPGKET
                     LYIQPQRTFFLPDRNRIFTMYIRGAGRAHHVFALFEGPNKIKQEIFVASLDFKGWKRF
                     EVVIPPYLRLRNPKKYNRYELYFLGLKVQSYFRDEPGTSVFNLAEMFILADTSDRLIP
                     GADMPPMK"
     gene            2431..>2966
                     /gene="lpxK"
                     /locus_tag="PLY93_10925"
     CDS             2431..>2966
                     /gene="lpxK"
                     /locus_tag="PLY93_10925"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRP70034.1"
                     /translation="MNPLEKLYSFGDRLNKRLKKSVRIPGTRILSVGNITTGGTGKTP
                     ATIYFSKLLRSDYPTQAVLSRGYGGKLSHKTTLLSDGYKLLMPSDDSGDEPYLMAVNL
                     PGVKIAVGRNRLKNAQQLLRDSNISLFLLDDGFQHYRIARDADVVLVDATNPFGSGQL
                     LPNGILREPMKIWRALRR"
BASE COUNT          766 a          673 c          697 g          830 t
ORIGIN      
        1 tcttattctc gacgaaccca ttagcgcgct cgatgtttcg attcaagccc agatattaaa
       61 cttgctcgtc gatttacgtc agcaatatgg cttcgctgcg attttcatca cgcacgattt
      121 atcgattgtg cgctggttct ccgaccgtgt ggcggtgtta tatttaggtc gtgttgtcga
      181 aagtgcgctg gcggacatta tttttgaaaa gagccgtcat ccttatacaa aggcgctttc
      241 gctttcaaag ctcgacacta acgttcataa aaaagagttt ttttcgctgc aaggggaaat
      301 cccatcgccc atcaacgtgc cgtcggggtg tccttttgcg ccacggtgcg atcgtgttgt
      361 tccacagtgt acggcgtctt tcccaccgat aactaaaacg ggcaaagatg ttttttttca
      421 ttgctataat ccacttgagg tataaatgag cgtaaaaatt ttttcagagc acgcgttatt
      481 gcaaacgaaa attgcaacac tgcgcaatgt caatacgccg caccccgaat ttgtgcgcac
      541 gatgcaagat gtgagtacaa ttctttgtgc gctcgtgagt gcagaaatca ccacaacaaa
      601 gatgagcgta aagacgccgc tcacgcgcac gcatggcgtt aaagtagaat accacgtcgt
      661 gcttgtacca attttacgtg ccggtttagg gatgctcgag ggttttcaat cgcttttgcc
      721 cacggcgtcg gtggggcatg ttggcgttga gcgcgaccac aaaacactca aacccaaatt
      781 ttattattac aaagtgccca cgctgagagg cgccgaggtg tgggtcatcg accccatgct
      841 tgcaaccggc ggctcgctcg attttgcgct cacaaaaatt aaagacgcca agccgcgcat
      901 catccgtgcc gtgagcgttt tagcttctcc cgaggggatt gcttttgtac aaaaaaatca
      961 ccccgacgtg catttctaca cggccgtgct cgatacaaag ctcaacaaca aagggtatat
     1021 cgttcctgga cttggcgatg cgggtgatcg atattttaac acataatgta taaagcaatc
     1081 acgcgacaag cttctctcaa ccggcttttg ttggagcacg tcgtgccgcg ttttgagcga
     1141 gtgaaaaaaa ttcgattttt gtgcgcaaaa aaaacgattc atttacgtgg catcatccgc
     1201 gaaggcgacg aacgcatttt cggtatgaaa acgggcgata tttattttga ctttcgtatg
     1261 ttaaaagagg aagttggcgc acagtcggtg aaaatgcgtt tgagtaaaac aaaactcatg
     1321 agctctgcac gccccgatat gatcaagtgg agcaatcggt tctctaataa aattgaagat
     1381 attttgttgt atcattttac tcgcggcgat tcgccttttt ttatggtgaa tcgaaaaaga
     1441 cgtttcatcg gtttcgattt gaattttatt cttatgcaaa tacctcccga gttagacgtg
     1501 ttgggaaaaa ttaacatcgt gaacgtttct tttgaaccac ggcggattgt ctggtactta
     1561 aactcaaatc ttgtgttgca atcggtcgtg cagatgttta agccagagta tattgaaatt
     1621 gaaaaaattg aagccgaggc ggatgcacag cggcttttaa cggacatcaa tttaatcgaa
     1681 taatttattt cataggtggc atgtcggcgc cggggattag tcgatccgag gtgtcggcaa
     1741 ggataaacat ttctgctaaa ttaaaaacag acgtaccggg ttcgtctcta aaatagcttt
     1801 gtacttttaa tcctaaaaaa tataattcgt aacggttgta ttttttggga ttgcgcaacc
     1861 gcaagtatgg tgggattacc acttcaaaac gtttccatcc tttgaagtcg agtgatgcca
     1921 caaaaatttc ctgtttaatt ttatttggtc cctcgaaaag agcaaagaca tggtgtgccc
     1981 gtccggcgcc acgtatgtac attgtaaata tgcgattacg gtcgggtaaa aaaaatgtac
     2041 gctgcggttg gatatagagc gtttcttttc cagggtgcat gacattccag cggtattcgg
     2101 cagaggcgta cgtgggggtg agtgctgccc ttttttctat ggcaagcgtg tcgttcacgt
     2161 gctccaaatc gtttgcaaaa tttgcctcaa gatatttttt tttatcttta gtgggatgca
     2221 cactggtcat gcgcgcattg acgtggtgca gtgaatttct gtccggtgca aattctttga
     2281 ggttgaaatc cgttaccagt cggaatgcat ttgttgcatc actggcgagt ccgtaccaat
     2341 ttttcgcaaa agtcaaaggc aacataaaga ttgtaaaaaa tattaccgct cgacacgctc
     2401 tcgcaaaaac cacgaggtgc tctaatactt gtgaatcctc tcgaaaagct ttattctttc
     2461 ggcgatcgcc tcaacaaacg cctcaaaaaa tctgtgcgta taccgggtac gcgtattctt
     2521 tctgtgggca atattacaac cggcggcacg ggcaaaacac cggcgacaat ttatttctcc
     2581 aaactcctgc gctccgatta tcccactcag gcggtgctct cgcgcggcta tggcggtaag
     2641 ctttcgcaca aaacaacgct gctgtccgac ggctataaac ttttaatgcc gagcgacgac
     2701 tcaggcgacg aaccatactt gatggcggta aatttgccag gggtgaaaat tgcagtgggg
     2761 cgcaaccgct taaaaaacgc gcagcaactt ttacgcgatt caaatatctc tctctttttg
     2821 ctcgatgacg gttttcaaca ttaccgcatt gcgcgcgacg ccgacgtggt gctcgtcgac
     2881 gcaaccaatc ctttcggtag cggccaatta ttgcccaacg gtattttgcg cgaaccaatg
     2941 aaaatttggc gcgcgcttcg gcggtt
//
LOCUS       DAOMPD010000339         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13514_length_2964_cov_3.444826, whole genome shotgun sequence.
ACCESSION   DAOMPD010000339 DAOMPD010000000
VERSION     DAOMPD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13514_length_2964_cov_3.444826"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1435)
                     /locus_tag="PLY93_10930"
     CDS             complement(56..1435)
                     /locus_tag="PLY93_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70035.1"
                     /translation="MPRTKAIQNPASFRDPAGFIFHAEKKIFRTVSHSYREHYEHLME
                     SGLYEKLSGTQILVPHKEIPLKKFSHKYKKVYKILEPEKIDFISYPYEWCFSQLKDAA
                     LLTLEVQRTAFEHGMTLKDASVYNVQFQNGRAIFIDTLSFEKYIDGTPWVPYGQFCRH
                     FLAPLLLMTHTDVRLIRLLLLYTDGIPLDLASRLLPKRTYLSLSALVHLHIHSKTNSG
                     ISAVKKIYRIERNALAVLIDNLHNIVQGLQWKPRGTTWANYASFNNYSSRAVEHKKRI
                     VKEFIKYCNPTSVWDLGANVGIFSRIASSKNIPTYSFDIDPAAVEKNYCAIKKNTPEK
                     LLPLFLDLTNPTPAVGWANEERAALLTRAKPHTILALALIHHLVIANNVPLEKVAAFF
                     ANHCQHLIIEFVPKEDSQVQRLLASRADIFRDYTQKTFEKIFSHYFKIKKHESVEEST
                     RTLYWMHRR"
     gene            complement(1438..2334)
                     /locus_tag="PLY93_10935"
     CDS             complement(1438..2334)
                     /locus_tag="PLY93_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRP70036.1"
                     /translation="MPIFFLIVICFIAGILLRRFKKVPENTHVVLNNFIIYVSLPALI
                     IKSLRIIPMTTVYFSAALMPWLIFGSSVVFFLALQKLKVFDRLTTAALILTAGLGNTS
                     FVGFPVIEALYGKQYIPVAVIIDELGTFLVMCVMAIPLADFLRIGEFNLRKAIKDLAL
                     FPPMIALAFAFLFQAWRPPETVFTIAERLGDTLAPLALISVGFQWQASEMKELKKELA
                     VGLSYKLLLAPLLTAFVFSLFTYNAELKKIVVLEAAMGPMITGGIIAIQKNIHPKLVA
                     AMLSIGIPLSLLTSLVVKYFYK"
     gene            complement(2334..>2964)
                     /gene="aroC"
                     /locus_tag="PLY93_10940"
     CDS             complement(2334..>2964)
                     /gene="aroC"
                     /locus_tag="PLY93_10940"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRP70037.1"
                     /translation="KDIFYTDALDLTDPRTAREKIEANDIRVPDAALAGKMTRLIQDT
                     RQAGDSLGGVVTCHALGVPTGWGDPVFDRLDALIAHAMLAIPATKGIEIGSGFSAASL
                     TGSEHNDIFENQNGKIRTKTNRSGGIQGGISNGMPIEFRVAFKPTATISREQESVNAK
                     GENVTMKVKGRHDPCVLPRAVPIVEAMLALVLADQFLLAQSVRFDDLRV"
BASE COUNT          786 a          647 c          747 g          784 t
ORIGIN      
        1 gtttacttca aaccgtagtt ttgagatgcc gcgtcaacga ggtagttatc caatgttaac
       61 gtctgtgcat ccagtaaagc gtgcgtgtcg attcttccac tgattcgtgt tttttaattt
      121 tgaaatagtg cgaaaagatt ttctcgaaag ttttctgagt gtaatcgcgg aaaatatctg
      181 cacgtgaagc gagtagtcgc tgtacttgcg aatcttcttt cggcacaaat tcaataatta
      241 agtgttggca gtggtttgca aaaaacgccg ctactttttc caagggcacg ttgttcgcga
      301 taacgaggtg gtggatgagc gcaagcgcca aaatggtgtg tggtttcgcg cgggttagga
      361 gcgccgcacg ttcttcgttc gcccaaccca cagcgggcgt tggattagtc aaatcgagaa
      421 aaagggggag caatttctcg ggcgtatttt tttttatggc gcagtaattt ttttccactg
      481 ccgcagggtc gatgtcgaac gaatacgtgg ggatattttt agaagaagcg atgcggctaa
      541 agatgcctac atttgcgccc aaatcccaca ccgatgtggg gttgcagtac tttataaatt
      601 ctttaacaat ccttttctta tgctccactg cacgggatga atagttattg aaactggcat
      661 aattcgccca cgttgtgcca cggggtttcc actgcaaacc ttgaacgatg ttgtgcaagt
      721 tatctatcaa caccgctaaa gcgttacgct cgatgcggta tatttttttc actgcgctta
      781 taccgctatt tgtttttgag tggatgtgga gatgcaccaa tgcgctcaaa gaaagataag
      841 tgcgcttggg taaaagacgg ctagctaagt cgagtggaat accgtcggtg tagagtaaaa
      901 gcaaccgaat caagcgcacg tcggtgtgcg tcatgagtaa aagcggcgcc agaaaatgtc
      961 gacaaaactg cccgtagggg acccatggcg tcccatctat atatttctca aatgaaagag
     1021 tatcgataaa aatggcgcgg ccattttgga attgtacatt gtacacgctc gcgtctttga
     1081 gagtcatccc atgctcaaac gccgtgcgtt gaacttcaag tgtcaagagc gcagcgtctt
     1141 taagttggct gaaacaccat tcgtaagggt acgaaataaa atcaatcttt tcgggttcta
     1201 aaattttata tactttttta tatttgtgcg aaaatttttt tagtggaatt tctttatgcg
     1261 gcacaaggat ttgcgtaccg gataattttt catagagccc cgattccatt aagtgttcgt
     1321 agtgttcgcg gtacgagtgc gacaccgtac gaaatatttt tttctcggcg tggaaaataa
     1381 aaccagcagg gtcgcggaaa gaagcaggat tctggattgc cttggtgcga ggcatagtta
     1441 tttataaaag tatttcacca caagcgaagt caggagcgaa aggggaatgc cgatcgatag
     1501 cattgcggcg acaagcttag ggtggatatt tttctgtatt gcgatgatgc cgccggtgat
     1561 catgggaccc atcgccgctt cgagtacgac aatttttttg agttcagcgt tgtacgtgaa
     1621 taaagaaaac acaaacgcag tcaaaagcgg cgcaagcaaa agcttgtaac ttagaccgac
     1681 agcgagctct ttcttgagtt ctttcatctc gctcgcttgc cattgaaaac caactgaaat
     1741 gagtgcgagt ggggcgaggg tgtcgcctaa ccgctctgcg atggtgaata ctgtttcagg
     1801 tggtctccac gcttggaata aaaacgcaaa cgccaaagca atcatcggcg gaaagagcgc
     1861 gaggtcttta attgctttac gcaaattaaa ttcaccgatt cgaagaaaat ccgccaaagg
     1921 gattgccatc acgcacatca ccaaaaatgt accgagctcg tcaatgatga ctgcaacagg
     1981 gatgtattgt ttgccataaa gggcttcaat caccggaaag ccgacaaacg acgtgttgcc
     2041 caaccctgct gtaaggatga gcgcggcggt ggtgaggcga tcgaacactt tcagtttttg
     2101 taaagcgaga aaaaatacaa cagaactgcc gaaaataagc catggcatga gtgctgcgct
     2161 aaaataaacg gttgtcatag gtattatgcg caatgacttg atgatgagcg ctggtagtga
     2221 gacgtagatg ataaaattat tgagaaccac gtgtgtgttc tctggcacct ttttgaaacg
     2281 gcgtaaaagg atacctgcga taaaacaaat gacaatcaag aaaaaaatag gcattatacc
     2341 ctcaagtcgt caaagcgcac actttgtgca agcaagaatt gatctgccaa caccaacgcg
     2401 agcatcgctt cgacaatagg taccgcacgc ggcagcacgc aagggtcgtg gcggcctttc
     2461 actttcatcg taacgttttc gccttttgca ttcaccgatt cttgttcgcg gctaatcgtc
     2521 gcggtgggct taaacgccac ccgaaactca atcggcatac cgttggaaat tcccccttgg
     2581 atgccgcccg agcggtttgt tttggtacgg atttttccgt tttggttttc aaaaatatcg
     2641 ttgtgctcgc tgcccgtgag agacgcggca gaaaatccgc taccgatttc aatccctttt
     2701 gttgcaggga ttgcgagcat cgcgtgcgcg atgagtgcgt cgaggcggtc gaacaccgga
     2761 tcgccccaac cggtaggcac acccaatgca tggcaggtga caacgccgcc taaagagtcg
     2821 ccggcttgcc gtgtatcttg gatgaggcgt gtcatttttc cagcgagcgc agcatcgggc
     2881 acacggatat cgtttgcttc aattttttcc cgcgcggtac gtgggtcggt taaatcgagc
     2941 gcatcggtat aaaaaatatc ttta
//
LOCUS       DAOMPD010000340         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13622_length_2948_cov_2.899758, whole genome shotgun sequence.
ACCESSION   DAOMPD010000340 DAOMPD010000000
VERSION     DAOMPD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13622_length_2948_cov_2.899758"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLY93_10945"
     CDS             <1..195
                     /locus_tag="PLY93_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HRP70038.1"
                     /translation="HAAFLKPVEGRTEGVLVDSIKAMTELREKFDRVYGACASGGHTL
                     FDVEKGEGTLKELIAFSVAI"
     gene            200..430
                     /locus_tag="PLY93_10950"
     CDS             200..430
                     /locus_tag="PLY93_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70039.1"
                     /translation="MNTQSLISNLKRWQPTARERYKTELVGLFGSWASLFDWVALAQA
                     MENEFHPRIDLVLEKNFKPRIKKYIEPELIQL"
     gene            427..1176
                     /gene="cas5e"
                     /locus_tag="PLY93_10955"
     CDS             427..1176
                     /gene="cas5e"
                     /locus_tag="PLY93_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HRP70040.1"
                     /translation="MREYLLFRLYGPLASWGDIAVGEYRPSYSYPSKSAICGLLAAAL
                     GIDRIDDESHLDLSRSVQFALCVLAPGSLLRDYHTIQTPGNTRRHLSTRKDELADRLN
                     IATILSSRDYRMDAAYDACISLKEDSQYDLAQIKGALERPKYTLYLGRKSCVVALPLC
                     PRLLQAESALRALQAYETVLKDQEGGIKSEVSGLLAGGNSVTYLWEEDNSDEYTHIQT
                     RRDSVLSRRRWQFAERQEKYKMIGKERDIHV"
     gene            1189..1650
                     /locus_tag="PLY93_10960"
     CDS             1189..1650
                     /locus_tag="PLY93_10960"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRP70041.1"
                     /translation="MSFYEKLLNKILLGQSDANIEFTELCKLLEKLGFSMHITGSHHV
                     FRKEGVRELINVQKDGGKAKPCQIPGQANFSRLQFQGVVMNKYEIIIFYSNEDEAFIA
                     EVPELPGCMAHGATNEEALRSANDAIAAWLETAKKFGDPIPQPKGRKLMFA"
     gene            1660..2454
                     /gene="cas6e"
                     /locus_tag="PLY93_10965"
     CDS             1660..2454
                     /gene="cas6e"
                     /locus_tag="PLY93_10965"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HRP70042.1"
                     /translation="MYLTRLTLDPRSQAIQNWLSNPYRIHQRLWRGVPEIIQRQHEAL
                     TSATPPFLYKVEIKPQLGMLVQSLHKIDWGSVFADEKILKPLTEDTKILTPEKFIVAG
                     RSYRFSLLANATKKVKDYRSVFAAELSGYPVTSGRVDRADYKEGKVRLEELKKTLSKE
                     QKERIKSKKVGVYSEMEQLAWLARRGMAGGFQLQSAIVESTQTISATKKKMEGGGGEI
                     RKILAVTFAGLLEVTDSETFIKTYAAGIGSAKAFGCGLLLLAPARA"
     assembly_gap    2508..2517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2573..>2948
                     /locus_tag="PLY93_10970"
     CDS             2573..>2948
                     /locus_tag="PLY93_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HRP70043.1"
                     /translation="MANRNLQEIPKFGDNWSFLYFEKGTLDQWQKSVAFHYTLASGEE
                     KYIQIPIETVALLMLGPGTTVTHEAIKRVSESRCLMAWTGERGVRFYSAGYTGTYSSR
                     NLLKQIQLHEDPTQRRRVVHDMY"
BASE COUNT          789 a          680 c          764 g          705 t
ORIGIN      
        1 cacgccgctt ttttaaagcc tgtggaaggt cgaaccgagg gtgtattggt cgactcgatt
       61 aaagcaatga cggagctccg ggagaagttc gacagagtct atggcgcctg cgcttccggt
      121 gggcatactc tcttcgatgt agagaaggga gaaggcacgc tgaaggagct gattgctttt
      181 tctgtggcca tataataata tgaacaccca atcgcttatc tctaacttaa aacgctggca
      241 accgaccgca cgggagcggt ataagaccga attggtaggt ttgttcggtt cctgggcttc
      301 gctctttgat tgggtggcgt tagcgcaagc tatggagaat gaattccatc ctaggataga
      361 tttagttctg gaaaaaaact ttaagccgcg gatcaaaaaa tatatcgagc cggagttgat
      421 ccaactgtga gagaatattt actcttcaga ctctatggtc ctttggcctc ttggggagat
      481 attgccgtcg gagagtaccg gccaagctat tcttatccat ccaagtcggc gatctgcggt
      541 cttcttgcag ccgccttggg tatcgatcgc atagacgacg aaagtcatct tgatctttcg
      601 cgatccgttc agttcgcatt gtgcgttctt gctccgggta gcctgctccg cgactaccac
      661 accatacaga ctccgggaaa tacgcggcgc catctttcga cgcgcaaaga tgagttggcg
      721 gatcgcttaa acatcgcgac tattttatcc agccgggatt atcgcatgga tgcggcgtat
      781 gacgcctgca tttctctgaa agaggattcc caatacgatt tagcgcagat caagggcgct
      841 ctcgagcgcc ctaagtacac tctctatctt ggtcgtaagt cctgtgtggt ggcgttaccc
      901 ctttgccccc ggcttttgca ggcggaatct gcgctgcgtg cattacaagc ttatgaaact
      961 gtattaaagg accaagaagg ggggataaaa tcggaggtaa gcggattact tgcaggcggc
     1021 aattcggtaa cgtatctctg ggaagaagat aactcagatg aatatactca catccagacg
     1081 cgccgcgatt ctgttttgag ccgccggcgc tggcagtttg cggagcggca ggagaaatat
     1141 aagatgattg gcaaagaaag agatattcat gtgtagagtg tttatactat gtcgttttat
     1201 gaaaagctac taaacaaaat tttgctcggt caatccgatg ccaatattga atttacggag
     1261 ctttgtaaac tcttggaaaa attgggattc tcgatgcata tcaccggatc gcatcatgtg
     1321 tttcgtaaag aaggtgtccg tgaactcatc aatgtccaaa aagatggagg caaggcaaag
     1381 ccctgtcaga ttccaggtca ggcgaatttt agtagattac aatttcaagg agtggttatg
     1441 aacaaatacg aaatcattat cttctacagc aacgaagatg aagctttcat tgcggaagta
     1501 ccggaactgc cgggctgcat ggcgcatggg gccacaaacg aagaggctct gagaagtgca
     1561 aacgacgcaa tcgccgcttg gcttgaaacc gctaaaaaat ttggagaccc aataccccag
     1621 cctaaaggta gaaagctgat gtttgcctga tttttcggta tgtacctcac ccgcctcaca
     1681 ctcgatcccc gcagccaggc aatccagaat tggctcagca acccgtaccg catacaccag
     1741 cgcttgtggc ggggcgtccc cgaaataatc caacggcaac acgaggcgct gacaagtgcg
     1801 accccgccgt ttctatacaa agtggaaatc aaaccgcaac tgggaatgct ggtgcaatcg
     1861 ctgcacaaaa tcgactgggg cagcgtattt gcagacgaaa aaattctcaa accgctaacc
     1921 gaggatacaa agattttgac acccgagaaa tttattgttg ctggacgtag ctatcgcttt
     1981 agcttgttgg caaatgcaac aaagaaggta aaagattacc gcagcgtttt tgcagcagaa
     2041 ctttcggggt atccggtaac ctctggtcgc gtcgaccggg cggattataa agaaggaaaa
     2101 gtcagattgg aagagctcaa gaagaccttg tccaaagaac aaaaagaaag aataaagtcc
     2161 aaaaaagtgg gggtttattc agagatggaa cagcttgcct ggctggcgcg ccgcggtatg
     2221 gctggcggct tccaattgca gagtgccatt gtcgaaagta cgcagacaat ttcggcgacg
     2281 aagaaaaaga tggaaggggg tggtggtgaa attaggaaaa tcctggccgt tacatttgcg
     2341 ggactcttgg aggtcaccga ctcagaaact tttatcaaaa cttatgccgc cggcatcggc
     2401 tccgccaagg cattcggctg cgggttgctc cttctcgcgc ccgcacgtgc gtgaggacag
     2461 gagtgagctg caacgcatag ccgcattata gcgacataat attttccnnn nnnnnnnttg
     2521 acatattttc ttttccattg acgcagcgag taaggcaggt ctatatcgac ctatggctaa
     2581 ccggaactta caagaaattc ctaaatttgg cgataactgg agttttcttt atttcgaaaa
     2641 aggcacgctc gaccaatggc aaaagtcggt cgcattccat tacactctgg ccagtggcga
     2701 agagaagtat atccagatac cgatagaaac cgtggctctc ttaatgctcg ggcccggtac
     2761 cacggtgacg cacgaagcga ttaagcgtgt ttctgaaagc cgctgtctca tggcgtggac
     2821 gggagagcgt ggggtccgtt tttactccgc cggttacacg ggcacctatt cgtcgcgcaa
     2881 tctattgaaa caaattcagt tacacgaaga tccaacacaa cgacgccggg tggtacacga
     2941 tatgtacc
//
LOCUS       DAOMPD010000341         2922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13770_length_2922_cov_2.531566, whole genome shotgun sequence.
ACCESSION   DAOMPD010000341 DAOMPD010000000
VERSION     DAOMPD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_2922_cov_2.531566"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLY93_10975"
     CDS             complement(<1..1209)
                     /locus_tag="PLY93_10975"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRP70044.1"
                     /translation="MKAQPLHDNSAVIVDGIRTPFVRAFQDYRNFDTIELGRRTVEAL
                     LKRNGIQNEVEAIYWGAVLLPSTTPNIAREIALETALKPTVESLTVTRACSSSLMSVT
                     QAAAAIERGEFDVALAGGSESVSNAEVPLPQKLIRTFAPLVMNSKSGPLDYLSALAQL
                     APFNDLLPKMPRVAERSTGELMGEAAENMCRRAGITREAQDELAYKSHVASSKAFESG
                     RFDDEIFPMEIKPGHTVYRDQIVRGNIEYKKLSQLRPAFRRRGTITAGNASALTDGAA
                     ATLLMSYKKARELGLKPRARFLSWHYSGVDPRDQLLIGPAISMPIALERAGLSMKDID
                     MVEIHEAFAGQVLCVLKAMASEEFVKFYAGDKAKASEVNPAFLNKYGGSVALGHPFGA
                     TGARIVNTAAN"
     gene            complement(1211..2617)
                     /locus_tag="PLY93_10980"
     CDS             complement(1211..2617)
                     /locus_tag="PLY93_10980"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HRP70045.1"
                     /translation="MQDIILSAAKNPLLRNLFSQVGLPTPVPLMRQKDAWVNESLHNK
                     KIVYWGSSSENNNALLTPLLEAGGEIYTDSKTNDALKKIATRVGTEIRAVSKLAEHDR
                     VYALVLDASALKTPDSFQTFYEFFNAHLARVEQNGRILVVSTCARGSDLVHECAIEGF
                     IRSIAKEVGAKAITANALLGEVKDEKALATPVLFFLSRSSCYVSGQILHLSATATAPK
                     EIQRAQALKGKVALVTGAARGIGEKIARALAREGARVVCLDREQEGDALARVARSIDG
                     YIFHQDLSEANAAEKISQYFKTKFKGCDILIHNAGITQDKTLKKMPKEKWQRVIDINL
                     KAPIAITEQMLKDKSLNDGCRVVCLSSIAGIAGNFGQTNYATSKAGLRGYVASMATTL
                     AKTHSTINAVAPGFIETKMTETIPLVTREAGRRLNNLGQGGHPEDVAEVICFLSQPGV
                     FGVNGETLRVCGGSILGA"
     gene            complement(2627..>2922)
                     /locus_tag="PLY93_10985"
     CDS             complement(2627..>2922)
                     /locus_tag="PLY93_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70046.1"
                     /translation="LAPYAQMSGFRRPILHGFAQLGRVYEHIRKNIFCGQIDPLAEMT
                     IRFEKPLVLPQACRVFLTDDGAFYIGAAVGSQAVTSGSVRAKKAAAKIPQAKN"
BASE COUNT          639 a          783 c          753 g          747 t
ORIGIN      
        1 attggcggcg gtgttcacga tacgcgctcc ggttgcgccg aaaggatgcc ccagcgccac
       61 cgagccaccg tacttgttga gaaatgctgg attgacttca ctggcttttg ctttgtcacc
      121 ggcataaaat ttcacaaact cctccgacgc catcgctttc aacacgcaca gcacttggcc
      181 tgcaaacgct tcgtggattt ccaccatatc gatgtctttc atactgagcc ccgcccgctc
      241 caaagcaatc ggcatcgaaa ttgcaggacc aatcaacagt tgatcgcgcg gatcgacacc
      301 gctgtaatgc cacgaaagaa aacgcgcgcg gggtttcaaa cccagctcgc gcgctttttt
      361 gtagctcatg agcagcgttg cggctgcgcc atcggtgagc gcgctggcgt tgcctgcggt
      421 gatagtgcct ctacggcgaa acgcaggcct cagttggctt aattttttat attcgatgtt
      481 cccgcgcaca atttggtcgc ggtaaacggt gtgcccgggt ttgatttcca tcggaaaaat
      541 ttcatcgtca aagcggccac tctcaaatgc cttactcgat gcaacatgcg atttatacgc
      601 gagctcgtct tgtgcttcgc gcgtgatgcc cgcacggcgg cacatattct ccgctgcttc
      661 acccatgagc tcaccggtgg agcgttcggc gacgcgtggc attttcggta ataaatcatt
      721 aaacggtgca agctgcgcca atgcgcttaa atagtcgagc ggccccgact tactgttcat
      781 taccagcggc gcaaaagtgc ggatgagttt ttgtggcagc ggtacctcgg cattcgacac
      841 cgattcagaa ccgcctgcga gcgcgacgtc aaactcaccg cgttcaatcg ctgctgccgc
      901 ttgtgtaacc gacatgagcg agctcgaaca cgcacgagtg acagtgagcg attccactgt
      961 gggtttaagc gcagtttcga gcgcgatttc acgcgcgatg ttcggcgtcg tcgagggtaa
     1021 aagcactgca ccccagtaaa tcgcttcaac ctcgttttga atcccattgc gctttaagag
     1081 cgcttccacg gtgcgtcgtc ctaattcgat ggtatcaaag ttgcggtagt cttggaacgc
     1141 gcgcacaaaa ggcgtgcgga taccatcgac gatgacggct gagttgtcgt gtaaaggttg
     1201 cgctttcatg ttatgcacct aaaatactgc cgccgcacac gcgcagcgtt tcgccgttga
     1261 cgccaaaaac tcccggctgc gacagaaagc aaattacctc agcgacatct tcgggatggc
     1321 cgccttggcc taagttattg aggcggcgcc ctgcttcgcg cgtcacgagc ggaatcgtct
     1381 cagtcatttt agtttcaata aaacccggtg ccaccgcgtt aatcgtcgaa tgggttttgg
     1441 caagcgttgt tgccatcgat gcaacataac cgcgcaagcc cgctttcgag gtggcgtagt
     1501 tggtttgtcc aaagttgcct gcaatgcctg cgatggacga caaacatacg acgcggcaac
     1561 catcgttgag ggatttatct ttcaacattt gctcggtgat cgcaatcggt gctttgagat
     1621 tgatgtcgat gacacgctgc catttttctt tgggcatttt tttgagcgtt ttatcttggg
     1681 tgatgcctgc gttgtggatg agaatatcgc agcctttaaa ttttgttttg aaatactgcg
     1741 atattttttc tgcggcgttg gcttcactca agtcttgatg gaaaatataa ccgtcgatgc
     1801 tacgcgccac gcgtgcgagt gcatcgcctt cttgttcgcg gtcgaggcag actacacgcg
     1861 caccctcacg agcgagcgca cgcgcaatct tctcgccgat accgcgtgcg gcaccggtga
     1921 cgagtgccac cttacctttc agtgcttgcg cacgttggat ttctttcggt gcagttgcgg
     1981 ttgcacttaa atgcaaaatt tgtccactca catagcacga tgaacgcgac agaaaaaaga
     2041 gaaccggtgt tgcaagtgct ttctcatctt tgacttcacc caagagtgca ttcgcagtga
     2101 tagcttttgc gccgacttct tttgcgatcg atcgaataaa cccttcgatg gcacactcat
     2161 gcacgagatc gcttcctctg gcgcaagtcg agacaaccaa gatgcggcca ttttgctcga
     2221 cgcgcgcgag gtgtgcgttg aaaaactcat agaaagtttg aaagctgtcc ggtgttttga
     2281 gtgcgcttgc gtccaagacc aatgcgtaca cgcggtcgtg ttcagcgagc tttgaaacag
     2341 cacgaatttc tgttcccaca cgcgtggcaa tttttttaag cgcgtcgttt gttttggagt
     2401 cggtgtaaat ctcaccaccg gcctctaaga gcggtgtgag gagtgcgttg ttattttcgg
     2461 aggatgaacc ccaatacaca atttttttat tgtgtagact ttcattcacc cacgcatcct
     2521 tttgccgcat aagcggcacc ggtgtgggca atcccacttg tgagaaaaga ttacgcagca
     2581 gtggattttt tgccgcgctt aaaataatgt cttgcatagt tactccttaa tttttcgctt
     2641 gggggatttt ggccgccgct ttttttgcgc gcacgctacc cgaggtaacc gcttgcgagc
     2701 ccaccgcagc accgatataa aatgcgccgt cgtcggttaa aaacacacgg cacgcttgcg
     2761 gcaacacgag cggcttttca aagcggatgg tcatttctgc aagcggatcg atttgcccac
     2821 agaaaatatt tttgcggatg tgttcgtaca cacgccccaa ctgtgcaaag ccgtgcagaa
     2881 tcggacgccg aaagcctgac atttgcgcat acggcgctaa cc
//
LOCUS       DAOMPD010000342         2919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13789_length_2919_cov_2.888268, whole genome shotgun sequence.
ACCESSION   DAOMPD010000342 DAOMPD010000000
VERSION     DAOMPD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_2919_cov_2.888268"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLY93_10990"
     CDS             <1..724
                     /locus_tag="PLY93_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRP70047.1"
                     /translation="LPSSDVLKDENLPKGEFVLDGSLVARLLNPPEKNEYTPAFKLFR
                     YYSLFLNAWNQRVKLWPLLKNEKIFSLAVKNIETDTSASDLFFLSQHFATHKSWIPIL
                     MEVPVRRIKDAFHVDKDATAIYFRTLTKQVTQKEHPFLNDPPKMEVKNGTHVANLART
                     MRSELSRKGVQVLEFTNADHNDYEKTILLGTSGNAFYLESIARIIGVQKSYAAVNRSL
                     FTDLVLVIGKDYNKLKGEEVTQ"
     assembly_gap    735..775
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <776..1350
                     /gene="rsfS"
                     /locus_tag="PLY93_10995"
     CDS             <776..1350
                     /gene="rsfS"
                     /locus_tag="PLY93_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRP70048.1"
                     /translation="ATRSKANVKPDIDLQQIAILVRAIEAKKAEDVVVLDLREVSSYL
                     NYFVIATALSRAHLRTLYDELHKTAIENNIERPRAQTPQFDSGWVTYDFGFYVVHLFE
                     NEKRQFYDLESVWANAPRVQLDETSSHSSQGNLLQKKKKSVKTESVAKKKTITKKKTI
                     KEKTMATAKKKAAPKKKAAPKKKAAAKKKK"
     gene            complement(1421..1726)
                     /locus_tag="PLY93_11000"
     CDS             complement(1421..1726)
                     /locus_tag="PLY93_11000"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRP70049.1"
                     /translation="MSSQQNIPNENKIEIHLDPEILKGTFANVTNIGHAKEEFILDYL
                     FIQQHPAPFGKLVSRIILTPGHAKRLLQALQENVRRYEEQFGAIDTGVATPTNRTLQ"
     gene            1787..2347
                     /locus_tag="PLY93_11005"
     CDS             1787..2347
                     /locus_tag="PLY93_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP70050.1"
                     /translation="MIKKNCFRLFLFLTVLFTASACVDYSNSSSLVGTRGQIDDVPHY
                     DRFIGSIYFHTASSALSNSAIKDIKRIARTLNDRRYEKSRVVISGYADKKRGVEENTE
                     LAAERAQRVALTLEAQGLDLERLIIDSRDSRFARSKAAGRRVDIYIDYNSVGAYRNDF
                     LYPILVGFFLLVTFAVALVVFRRRSR"
     gene            2437..>2919
                     /locus_tag="PLY93_11010"
     CDS             2437..>2919
                     /locus_tag="PLY93_11010"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRP70051.1"
                     /translation="MVLPNATNATTRHYPLAEIWRKNPTRHSHCAAATHGVTERENNR
                     KFKIYMSESYYNYWGKSRPNADGDYDYHPLVYHSLDVAAVGWVWLTKEPGLLAKFGPA
                     LGCCDGNELKPLIAFFLGLHDLGKFADSFQGPNSDVRRALGKIGEAPVYNVRHDSLGY
                     L"
BASE COUNT          798 a          634 c          689 g          757 t
ORIGIN      
        1 tctaccatcg agcgacgtac ttaaagatga aaatctaccc aaaggcgaat ttgttttaga
       61 cggttctttg gttgcgcgcc ttttgaatcc accagagaaa aatgaataca ctcctgcgtt
      121 taagctcttc cgttattatt ctctcttctt gaacgcgtgg aatcaacgcg taaaactgtg
      181 gccgcttctt aaaaacgaaa agattttttc gctggcggta aaaaatatcg aaaccgatac
      241 atccgcctcc gatctttttt tcttaagtca gcattttgca acccacaaaa gttggattcc
      301 cattcttatg gaagtgccgg tgcgtcgaat aaaagacgcg ttccacgtcg ataaagacgc
      361 tactgctatc tatttccgca ctctcacaaa gcaagttact caaaaggaac acccgttctt
      421 gaatgaccca cctaaaatgg aagtgaagaa tggtacgcac gttgcaaatt tggcgcgcac
      481 catgcgcagc gaactttcac gcaaaggagt gcaagtgctt gagtttacaa acgccgacca
      541 caacgattac gaaaagacga ttcttttagg cacaagcggc aatgcgtttt atttggaaag
      601 tattgcgcgc atcatcggcg tacagaaatc gtatgctgcg gtgaatcgtt cgcttttcac
      661 cgaccttgtg ttggtgatcg gtaaagacta caataagctc aaaggcgaag aggtcactca
      721 ataatggcaa cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagcg
      781 acgcgcagta aagcgaacgt taaaccggat atagacttgc agcaaattgc tattttggtg
      841 cgcgcgatag aagctaaaaa agcggaagat gttgttgttc tcgatttgcg cgaggtctct
      901 tcatatctca attattttgt tatcgccact gcgctttccc gcgcacactt gcgcaccctt
      961 tacgacgagt tgcataagac tgccattgaa aataatatcg agaggccgcg tgcacagacg
     1021 ccacaattcg atagtggttg ggttacatac gattttggat tttacgttgt gcatttattt
     1081 gaaaacgaaa agcgacaatt ctacgattta gaatcggttt gggcgaacgc gccacgcgtt
     1141 caactcgacg aaacaagttc tcattcctcg cagggcaatc tgctacagaa gaaaaaaaag
     1201 tctgtaaaaa ctgaaagcgt tgcgaaaaaa aagacgataa ctaagaagaa gacgataaag
     1261 gagaaaacca tggcaacagc aaagaaaaaa gctgcaccta aaaagaaagc agcacctaag
     1321 aaaaaagcag cagcgaagaa aaaaaagtaa tcttcgccga ttctactaag gggcctttga
     1381 ttagaaaatt tctattcttt ggcccctttt tttttggttt ttattgtaac gtgcggtttg
     1441 tcggtgttgc gacaccggtg tcgatagccc caaattgttc ttcgtaacgg cgtacatttt
     1501 cttgcagcgc ttgcaataag cgcttagcgt ggccgggcgt taaaatgata cgcgacacta
     1561 atttcccgaa aggtgcgggg tgttgctgaa taaataaata atctagaata aattcttctt
     1621 ttgcgtggcc gatgttggtg acgttggcga aagtcccttt tagtatttca gggtcaagat
     1681 ggatttcaat tttattttca ttgggtatgt tctgctgcga actcatgcca acaaaaaaag
     1741 gaaggcttac tgcttgacaa cgacaaaaaa gaagcatgtt agaaccatga taaagaaaaa
     1801 ttgttttcgt ttgtttttat ttttgactgt gttgttcaca gcatcggcgt gcgttgatta
     1861 ttcaaattct tcgtcgctag tcggcacaag aggtcaaata gacgacgtgc cacattacga
     1921 tcgctttatc ggcagcatct attttcacac cgcttcgtcg gcgctctcga atagcgcaat
     1981 caaggacata aaacgtatcg cacgaacgct caacgatcga cgctacgaaa aatcgcgtgt
     2041 tgtgattagc ggctatgcgg ataagaaacg cggtgttgaa gaaaacaccg aacttgccgc
     2101 cgagcgcgca caacgtgttg cattgacgtt agaagcccaa gggcttgact tagaacggct
     2161 catcatcgac agccgcgact cgcgttttgc gcgctctaag gcagcggggc ggcgcgttga
     2221 catttatatc gattacaatt cggtgggcgc ctaccgcaat gattttcttt accctatact
     2281 tgtaggattt tttcttttag tcacatttgc cgtagcgctg gttgttttcc gtcggcggag
     2341 tagatagcgt aattggtgga gtgaaggtgt acgattacca tgctaactta agcataggca
     2401 agttccaggg gaactctcga tcgatggttg taatctatgg tcctaccgaa tgcaaccaac
     2461 gccactacgc ggcattatcc attggctgag atttggcgca aaaacccaac gcgccattcc
     2521 cattgtgctg cggccacgca tggggtcact gagcgcgaga ataatcggaa atttaaaatt
     2581 tatatgagcg agagttacta taattactgg ggcaagtcgc ggccgaatgc cgacggcgac
     2641 tatgactacc acccgcttgt ctaccacagc ctcgatgtcg cggcggtggg atgggtgtgg
     2701 cttaccaaag agccgggttt gctggctaaa ttcggcccgg cattggggtg ttgcgacggc
     2761 aatgaactaa aacccctgat tgcatttttt ctcggtttgc acgatttagg caaatttgcc
     2821 gattcgtttc aagggccaaa ttccgatgtg cggcgggcat tgggaaagat cggagaggca
     2881 ccggtttaca atgtgcggca cgacagcctt ggctattta
//
LOCUS       DAOMPD010000343         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_13902_length_2899_cov_2.048523, whole genome shotgun sequence.
ACCESSION   DAOMPD010000343 DAOMPD010000000
VERSION     DAOMPD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13902_length_2899_cov_2.048523"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLY93_11015"
     CDS             <1..466
                     /locus_tag="PLY93_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70052.1"
                     /translation="PQTKEKILVRTTIRRMINAMVEDLIYTTEKNLERAGIKNFATLL
                     VYTGDKKNPPLVCFSNVLDAQVKALKQFLFRYLYRHADVVQMNERAVITLSHIFDVIM
                     DNPRLLPPDFYERTKDVDTPRVIADFIAGMTDRYALEWKRLYDESSNRSIYP"
     gene            507..>1109
                     /locus_tag="PLY93_11020"
     CDS             507..>1109
                     /locus_tag="PLY93_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70053.1"
                     /translation="MRNLNYYLTFFAFLATYSLSAQRLGRPADKVPLIKTKKAEPTTP
                     LLADDGSVTSVKNTEESGGDTSRMATAEKLKADREKSAEDRLPFGPPQIEKRENVFGD
                     NKEKRRILDKMEYRIGFITEGAGFNNTDLRKLDESTVATIQNTDDKQTFAFSRFYINL
                     YFPLTENFYFRVDLFKNGFWGNHQLAGSSANNSSSSTPVGA"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1120..1651
                     /locus_tag="PLY93_11025"
     CDS             <1120..1651
                     /locus_tag="PLY93_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70054.1"
                     /translation="MSFGDLHLNYVIWKNAQNDLSIKIGRQFFEIGGEVYDYFLKDYL
                     DAVTLNYANSNIGGFRILAFDAFQMGADPTLHANYVRYFSHDNYRVRYFDGDVNVLRA
                     GIIYDSRNFLNWKGPLAGDLLQSKLYAFGARYGAVAKGGSDRSHRGDRVTFAITISIL
                     CLAHASFLISPANDTF"
     assembly_gap    1791..1822
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1823..2369
                     /locus_tag="PLY93_11030"
     CDS             <1823..2369
                     /locus_tag="PLY93_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70055.1"
                     /translation="ANADGFFASGGKYDANGIQTSHGFTSFKGSRVGGLIMSRYYGFR
                     PSSYTDAGGFADYPHDYERKASTAFAHLGVGTTLWQKIRFGLDYWIAFDTGSSERVKT
                     MATDPITNVVIKAQERLGKLMGQEVNFSTDFAVNQFWTLYFKAGVFSPGAFYSTPGIV
                     TDTPYGTDIAMGVQLGSKLVF"
     gene            complement(2398..>2899)
                     /locus_tag="PLY93_11035"
     CDS             complement(2398..>2899)
                     /locus_tag="PLY93_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRP70056.1"
                     /translation="QSQVKSIPNLTQQYLHQSEQIATLMLTGMKLRHSARRGLTAEHC
                     FGLLIEALPDVSEEKKFIITDNLERLTSLTQYFDGKEKNGEPAKDVMDLLDDIYPGEQ
                     WIQSGEIPLVYRCNCHREGYLERIVELSRVDIENVFGDLAEIEVKCGYCANKFYYKRS
                     EIESYL"
BASE COUNT          716 a          678 c          694 g          769 t
ORIGIN      
        1 cccgcagaca aaagaaaaaa ttttggtgcg caccaccatt cgaagaatga ttaacgcgat
       61 ggttgaggat ttaatctaca caaccgaaaa aaatttagag agggcgggta taaaaaattt
      121 tgctacgctt ctcgtctata ccggcgataa aaaaaatccc cctcttgttt gtttttcaaa
      181 tgttctcgat gcacaggtaa aagcgcttaa acaatttttg tttcgttatt tataccggca
      241 cgccgatgta gtacagatga atgagcgtgc ggtgatcaca ttgtctcaca tttttgatgt
      301 tattatggat aatccacgat tgttgccccc tgatttttat gaacgcacta aagatgtcga
      361 taccccccgc gttattgccg attttatcgc tggcatgact gatcggtatg cgcttgaatg
      421 gaaaaggttg tatgatgaat cgtcaaaccg atccatatat ccgtaaaact gttgacacga
      481 acgcaaagaa acgaatgttt tactacttgc gtaatttaaa ttattattta acattttttg
      541 cgttcttggc aacgtattct ttgtcggcgc agcgtttagg gcgaccggcg gataaagtac
      601 cgttgatcaa aacaaaaaaa gcggagccaa caacgccttt actcgccgac gacggtagcg
      661 tcaccagcgt aaaaaatacg gaagaaagtg gcggcgacac gagtcggatg gccaccgctg
      721 aaaaattaaa agccgaccgc gaaaaatctg ccgaggaccg tttgccgttt ggaccgcctc
      781 aaattgaaaa gcgcgagaat gtttttggcg ataataaaga aaaacggcgc attctggata
      841 aaatggaata ccgcatcggt tttattaccg aaggcgcagg ctttaacaat accgatttgc
      901 gcaagctcga tgaatcgacg gtcgccacca tccaaaacac cgacgacaaa cagacatttg
      961 cattttcacg cttttatatc aacctttatt ttccgttgac agaaaatttt tatttccgcg
     1021 tcgacctttt caaaaacggt ttttggggta atcaccagct tgccggttca agcgccaaca
     1081 actcgtcgtc gtccaccccc gtgggcgccn nnnnnnnnng atgagctttg gtgatttaca
     1141 cttaaattac gttatttgga aaaatgcgca aaacgacttg tcgataaaaa ttggccgcca
     1201 gttttttgaa atcggcggcg aggtgtacga ttactttttg aaagactatc tcgatgcggt
     1261 gacgttgaac tacgccaatt ccaacatcgg tggttttcgt attcttgcgt tcgacgcatt
     1321 ccaaatgggg gcagatccca ctttgcacgc taactacgtg cgttattttt cacacgacaa
     1381 ctaccgcgtg cgctattttg acggtgacgt gaatgtgcta cgcgctggga ttatctacga
     1441 ttcgcgcaat tttttgaatt ggaaaggtcc gctcgcgggc gacttgctgc aaagcaaact
     1501 ctacgctttt ggcgcacgct acggcgctgt cgctaaaggc ggctctgacc gctcgcacag
     1561 gggggatcgg gtaacttttg cgataacgat ttcaatttta tgtttggcac acgcctcatt
     1621 tttaatctcc cccgcaaacg atacgttcta aaaacttttg ccgacgccgc cgtttcggtg
     1681 ggtatcgacc gtcgcttacc gacagccggt ggtgaaagcc aagacgtgga cacgaatggg
     1741 tttggctttg gcggcggtgc cagttttgaa ttgccgcgtg tcatccccat nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nncgcgaacg ccgacggttt ttttgcctcg ggcgggaaat
     1861 acgacgccaa cggcatccaa acttcgcacg gcttcaccag cttcaaaggc agccgcgtgg
     1921 gtggtctcat catgagccgt tactatggct ttcgtccgtc gagctatacc gacgccggtg
     1981 gtttcgccga ctatccgcac gactacgaac gcaaagcaag cacggcgttt gcgcacttgg
     2041 gggtgggtac aacgctttgg caaaaaatcc gtttcggtct cgactattgg attgcgttcg
     2101 acaccggatc gagtgagcgc gtcaaaacaa tggcgaccga ccccatcacg aacgtggtca
     2161 tcaaagcgca agagcgattg ggtaaactca tgggacaaga agttaatttt tccaccgact
     2221 ttgcagtaaa tcaattctgg acgttgtatt tcaaagcagg cgttttctcg cccggagcat
     2281 tttattcaac gccagggatt gtcaccgata caccgtacgg caccgatatc gcgatgggag
     2341 ttcagttggg ctcgaaattg gtgttctaac aatagccttt gagcctcgtg cggcaattta
     2401 caaatacgat tcaatttcac tgcgcttgta gtaaaattta ttggcgcagt aaccgcactt
     2461 gacttcaatt tccgctaagt cgccgaacac gttttcaata tcgacgcgcg aaagttcgac
     2521 gatgcgttcg aggtagcctt cgcggtggca gttgcaacgg tacacaagcg gtatctcccc
     2581 cgattgaatc cattgttcgc ccgggtaaat gtcgtcgagt aaatccatca cgtctttggc
     2641 gggttcgccg tttttttctt taccgtcgaa gtattgcgtt agcgaagtga ggcgttctaa
     2701 gttgtcggtg atgataaatt ttttttcctc cgaaacgtcg gggagtgctt cgatcaacaa
     2761 cccaaagcaa tgttctgcgg tcaatcccct gcgcgcgctg tgccgtagtt tcatgccggt
     2821 gagcatgagc gtggcaattt gttcgctttg gtgtaagtac tgctgcgtta agttggggat
     2881 agacttcact tggctttga
//
LOCUS       DAOMPD010000344         2863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14109_length_2863_cov_2.475783, whole genome shotgun sequence.
ACCESSION   DAOMPD010000344 DAOMPD010000000
VERSION     DAOMPD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14109_length_2863_cov_2.475783"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PLY93_11040"
     CDS             <1..936
                     /locus_tag="PLY93_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70057.1"
                     /translation="PTGTSSRRVQGRRVADVGELSSPARTGGKYHTGAKALEAPQKEE
                     VNFSFGYKGISNYLLQFNINARAYRKLFEVRYQDGSQVSTTASTAGAGVPVYNVIASG
                     NETYTLTNMDKNAYYTHFEIAVLQLPSASNWIFHGSIGGYFGAGYSPQGLAAFYNDVG
                     VYNESTADPNFRENRFGRLDNDRGYMGKIVFGYRFLKTLSVVNVLRYRDGEPMVKDRL
                     VTGLAQGPIVVPITERGGGLSGVGRHTYSLAWDLRLRYDAALWGNTVWAFLDVYNLLN
                     SRTELAEYPLEGTAFRDPVEQGIERTVRLGLGMQF"
     gene            complement(933..1607)
                     /locus_tag="PLY93_11045"
     CDS             complement(933..1607)
                     /locus_tag="PLY93_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRP70058.1"
                     /translation="MLPQLVIFDCDGVLVDTEDIYFAMNKKFINEQVKNASTAAAFTF
                     GYYEHFIGMASDLMWDEIRTKFNLRHSVEELIQIEKENKLAALQNAPIAPIDGVQSLI
                     QKLLEKNVNLAVASSGRREAVTLLLAKSNLMQYFPVIVTGEDVMRGKPAPDIFLKAAQ
                     LSKAPAGASVVIEDSRNGILGAKAASMRAVGYINPKSGSQDLSKADLRVQSFNDSALL
                     EFLSLT"
     gene            complement(1623..2357)
                     /locus_tag="PLY93_11050"
     CDS             complement(1623..2357)
                     /locus_tag="PLY93_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70059.1"
                     /translation="MDILSNPYLLVLFNALISGGAFLFFTLGIGSLAFKYRRRSSPYL
                     TLLCFVMGVTQLYAWLNLNTIIFQTAWANYIFVGANFFIGPGVYYFYQATAHENFTPS
                     RLTRWTFIPGVVVLFLIPLINWVAPQAMPKDPRQFFLTGTPSFIDVLFSAAMVHNIFY
                     YVKLFLVTRPLLKANPEIQKNGGATAFLLFFGIISFINLYGLVAYITQDIRHFYADSC
                     VITLLIVSILVFALRYPQYFLKVKPE"
     gene            complement(2392..>2863)
                     /locus_tag="PLY93_11055"
     CDS             complement(2392..>2863)
                     /locus_tag="PLY93_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRP70060.1"
                     /translation="AYYLGGIFPRHTVVVAKKEMKKVPLFGITLKASRTIFVDREDSS
                     DTKRALKQAIDTIKKDKNHIWIFPEGTRSQGKGLGEFKKGAFAMAIASGAPIVPIINE
                     PMERLLDIPNKTLHKGTQHVKILPAIPTEGMKFRDMDALHDKVRALFEKEIAAF"
BASE COUNT          748 a          688 c          731 g          696 t
ORIGIN      
        1 ccgacgggca cgtcgtcccg acgtgtgcaa ggacgacggg ttgccgacgt gggcgagctc
       61 tcttcaccgg cgcgtaccgg cggtaaatac cacaccggcg caaaagcgct cgaagcccca
      121 caaaaagaag aagtgaattt cagttttggc tacaaaggaa tttcaaatta tttgttgcag
      181 ttcaacatca acgcccgcgc ttaccgcaag ctctttgaag tgcgctacca agacggttcg
      241 caagtttcga caacagcatc gacggcgggg gctggcgttc cggtttacaa tgtcatcgca
      301 agcggcaacg aaacgtacac gctcacgaac atggacaaaa acgcgtacta cactcacttt
      361 gaaattgcag tgctgcaact tccctccgcg agtaactgga tttttcatgg ctccatcggc
      421 ggctattttg gcgcggggta ttcaccgcag ggccttgctg cgttctataa cgacgtgggt
      481 gtatacaacg aatcgactgc cgaccccaat tttcgtgaaa atcgttttgg ccgcctcgac
      541 aacgatcgtg gctatatggg gaaaattgtt ttcggttatc gctttcttaa aacactctcg
      601 gtggtaaatg tcctccgcta ccgcgacggc gaaccgatgg ttaaagatcg gcttgtcacc
      661 ggtcttgcgc aaggtcccat tgtggttccc atcaccgaac ggggcggcgg tttaagtggg
      721 gttggacgcc acacctactc gcttgcgtgg gatttacgcc tgcgctacga cgcagcgctg
      781 tgggggaata cggtttgggc atttctggat gtctataatc tcttgaactc gcgcaccgag
      841 ctcgccgaat acccgctcga aggcacagcg ttccgcgacc ccgtcgaaca aggcatcgag
      901 cggacggtac gcttggggtt agggatgcaa ttttaagtga gacttaaaaa ctctaaaagc
      961 gcggagtcat taaaactttg aacgcgtaaa tctgccttcg ataaatcttg cgaccccgat
     1021 ttagggttga tataccccac ggcgcgcatc gacgccgctt tagcacccag aatcccattg
     1081 cggctatcct cgatgaccac cgatgctccc gcaggagctt tcgagagttg ggcggctttt
     1141 aaaaaaatat cgggtgcggg cttgccgcgc atcacatctt caccggtgac aatcacagga
     1201 aagtattgca tcaggttact ttttgcaagc aataatgtta ccgcttcgcg ccgtcccgac
     1261 gaggcgacgg caaggttcac atttttttcc agcaactttt ggattaaact ttgaacgccg
     1321 tcgatgggag cgatgggggc attttggagc gcggcaagtt tattttcttt ttcaatttgg
     1381 attaactctt ctaccgaatg acgcaaatta aattttgtac ggatttcatc ccacattaaa
     1441 tcggacgcca ttccaatgaa atgttcgtaa taaccgaaag taaacgccgc agcggtggag
     1501 gcattcttca cctgttcgtt gataaatttt ttattcatcg caaaataaat gtcctcggtg
     1561 tcgacgagga caccgtcgca atcaaagatg acgagttgag gcagcatgtg ttcggctcgt
     1621 aattactctg gttttacttt caaaaaatac tgcggataac gcaatgcaaa aactaaaatc
     1681 gacacaatga ggagagtgat gacgcacgaa tcggcgtaga agtgccgtat atcttgggtg
     1741 atataagcga cgagaccata gaggttgata aaactaatga tgccgaaaaa caaaaggaac
     1801 gccgtcgctc ccccgttttt ttggatttcg gggttggctt taaggagtgg gcgtgtcacc
     1861 agaaaaagtt tcacatagta gaagatgtta tgcaccatcg ccgctgaaaa aagcacgtcg
     1921 ataaagctgg gggttccggt caaaaaaaac tggcgtgggt ctttcggcat cgcctgcggc
     1981 gcaacccagt taatgagcgg aattaaaaag aggacaacca cgcctgggat gaatgtccag
     2041 cgggttaacc gcgatggggt gaaattctcg tgggcggtgg cttgataaaa ataataaacc
     2101 cccggcccga taaaaaaatt cgcaccaaca aaaatataat ttgcccacgc ggtctggaaa
     2161 ataatcgtgt tgagatttaa ccacgcgtaa agctgtgtaa cccccatcac aaagcacagt
     2221 aaagttaaat agggcgatga acggcggcgg tacttaaagg cgagcgaacc tatacccagc
     2281 gtaaagaaaa gaaatgcgcc cccactaatg agcgcattga agagaacgag taaatacggg
     2341 tttgagagaa tgtccatgcg gggaaggtcg aaattcccgc agggcggtca actaaaacgc
     2401 cgcgatttct ttctcgaaaa gtgcacgcac tttgtcgtgg agcgcatcca tgtcgcggaa
     2461 cttcatcccc tcggtgggaa ttgcgggtaa aattttaacg tgctgcgttc ccttatgcaa
     2521 tgttttattt ggaatatcca aaagtcgttc catgggttcg ttgataatcg gcacaatcgg
     2581 tgcgcccgag gcaatcgcca tcgcaaacgc gccttttttg aactcgccca agcctttacc
     2641 ttggctgcgc gtgccttcag ggaaaatcca aatatggttt ttatcttttt tgatggtatc
     2701 gatggcttgt tttagcgcac gcttggtgtc gcttgaatct tcgcgatcga caaaaatggt
     2761 gcgcgacgct ttgagagtaa tcccaaagag aggtactttt ttcatttctt ttttagcgac
     2821 cacaacggtg tggcggggaa aaataccgcc taaataatac gcg
//
LOCUS       DAOMPD010000345         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14197_length_2849_cov_2.396922, whole genome shotgun sequence.
ACCESSION   DAOMPD010000345 DAOMPD010000000
VERSION     DAOMPD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14197_length_2849_cov_2.396922"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1195
                     /gene="pnp"
                     /locus_tag="PLY93_11060"
     CDS             <1..>1195
                     /gene="pnp"
                     /locus_tag="PLY93_11060"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRP70061.1"
                     /translation="MLAAVKFGHQEIIRLCEMQEKFKAKAGKPRWVVADKEDHSALNE
                     KIRALGLADMKAANAKKGKAERQEAIDATKKALQEKMAAEFASLEPADAEKQSKIVKS
                     LIGELEVDVVRDQIFHEGVRADGRKLDEIRPISIEVGVLPGAHGSALFTRGETQSLGV
                     LTLGTDGNAQEADDVEGKSSSRFYLHYNFPPYSVGEVRRISGPGRREIGHGKLAENSI
                     VSVLPDGARFPYVMRVVSEILESNGSSSMATVCSGALALMDGGVPIKAPVAGVAMGLI
                     TEGSGDNPKFAVLSDIAGLEDHFGDMDFKIAGSTKGITGFQLDTKVQGISYEIMEKAI
                     HQAREGRLHILNLMNAALDKPRGELKDNAPRISTLTIERDRIGELIGPGGKNIRSIIE
                     KTGVEI"
     assembly_gap    1196..1220
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1221..>1426
                     /locus_tag="PLY93_11065"
     CDS             <1221..>1426
                     /locus_tag="PLY93_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002147671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRP70062.1"
                     /translation="ASNDAESARKARSLIEGQFAEAEIGKIYEGTVKKITDFGAFIEI
                     MPGKDGLCHISKVSQQRIANVADV"
     assembly_gap    1427..1436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1437..1521
                     /locus_tag="PLY93_11070"
     CDS             <1437..1521
                     /locus_tag="PLY93_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRP70063.1"
                     /translation="KEGQRVNVKVLAVDRQGKISLSIRDAE"
     gene            1566..>2849
                     /locus_tag="PLY93_11075"
     CDS             1566..>2849
                     /locus_tag="PLY93_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRP70064.1"
                     /translation="MKVEKIRLDNGLTVLLENIPHRTSASVGLYLPIGSRFEKKSESG
                     FSHFVEHMLFKGTKNRDYTEIARAIDRLGGFMNASTGKEMTDYYVTLSGRHLSIALDV
                     ISDMFYNSLFSEEEFESEKKVIVEELKMTEDQPDDYLFDLFYRTQLGENSLGRPVGGE
                     STHIERTKRDTLFNYYRTHYGPNGAVLSLAGALFNSAQSKKSLIATIVRFFNRNNHTL
                     APKIGYKTEAYLKPEFQEKRTVKPKSFKHLPKKLEQVNFVLSLPGIKNDIKPTAEMSV
                     LTHYLGGTMSSRLFTEMREKKGLCYSVSAFHSQYLHEGIWGIFCATSPKKYADAVETA
                     VAEITKLADGISKHEIEESKSGLRGGTELSMESAHRRASFNARSELYYDTQIYWHEYI
                     AAIDRVTPESLQHTLLNLWRSVAPGLTSIGPKSSST"
BASE COUNT          764 a          683 c          714 g          653 t
ORIGIN      
        1 catgctcgct gccgttaaat ttggccatca agaaataatc cgcctttgcg aaatgcaaga
       61 aaagttcaaa gcgaaagcag gcaagcctcg ttgggttgtc gctgataaag aagaccattc
      121 tgcgctcaat gaaaaaattc gtgcattggg tcttgccgac atgaaagccg cgaatgcgaa
      181 aaagggtaaa gccgagcggc aagaagccat cgacgcgacc aaaaaagcgc tccaagaaaa
      241 aatggctgca gagtttgcat ccttggagcc tgccgatgca gaaaagcaat caaaaattgt
      301 gaagagtctt attggcgaac ttgaagtcga cgtggtgcgc gatcaaattt ttcacgaagg
      361 cgtgcgtgca gatggacgca aactcgacga aattcgaccc atctccatcg aagtgggcgt
      421 gttgcccggt gcacacggtt cggcgctttt tacgcgtggt gaaacgcaat cgttgggcgt
      481 gttgaccctt ggcaccgatg gcaacgcgca agaagccgac gacgtggaag gtaaaagttc
      541 aagtcggttt tatttacact acaacttccc tccttattca gtgggtgaag tccgccgcat
      601 ttcagggcct ggccgtcgtg aaatcggcca cggtaagctc gccgaaaatt cgattgtgag
      661 cgttcttccc gatggagcgc gttttccgta tgtgatgcgc gtcgtttctg aaattttaga
      721 atcgaatggt tcgtcgtcga tggcgaccgt gtgttcgggc gcactcgcgc tcatggacgg
      781 tggcgtaccg attaaagcgc ccgtagcggg tgtcgcgatg gggctcatca ccgaggggag
      841 tggcgacaat cccaagtttg ccgttctttc cgacatcgcc ggtttagaag accattttgg
      901 tgatatggat ttcaagattg ccggttcgac gaaaggaatt acaggattcc agctcgacac
      961 caaagtgcaa ggcatcagtt atgaaatcat ggaaaaggca atccaccaag ctagagaagg
     1021 gcgtctccat atcttgaacc tcatgaacgc tgcgctcgat aaaccgcgtg gagagctcaa
     1081 agacaatgcg ccgcgcatca gcacactcac cattgagcgc gaccgcatcg gcgaattgat
     1141 tgggccgggg ggcaagaata tccgctcgat tattgaaaaa accggcgttg agattnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn cgcctcgaac gatgcagagt cggcgcgtaa agcacgctcg
     1261 ctcatcgaag gccagtttgc cgaagcggag attggtaaaa tttatgaagg cacagtgaaa
     1321 aagattaccg atttcggtgc gttcattgag attatgccgg gtaaagacgg tctttgccac
     1381 atcagcaaag tatcgcagca acgcatcgcc aacgtggcgg atgtccnnnn nnnnnntaaa
     1441 gaaggacaaa gggtcaatgt gaaagtgttg gccgtcgatc gccaaggtaa gatatcactc
     1501 tccatccgcg acgctgagta actacacacg ctggttaccc atggcaacca gcgtgtgcta
     1561 taaacatgaa agtagaaaaa atacgactcg ataacggcct tacggtttta ctcgagaaca
     1621 ttccgcaccg taccagcgca tcggtggggc tttatctccc catagggtcg cgctttgaga
     1681 aaaaaagcga atcgggtttt tcgcacttcg tcgagcacat gcttttcaaa ggcacaaaaa
     1741 accgcgacta taccgagatt gcacgcgcca tcgatcgttt gggcggattt atgaacgcct
     1801 cgaccggaaa agaaatgacc gattattatg taacgttgag cggacggcac ctatcgattg
     1861 cactcgatgt aatttctgat atgttctaca attctctttt tagcgaagaa gaatttgaat
     1921 ctgaaaaaaa agtcatcgtc gaggagctca agatgactga agatcagcct gacgattatc
     1981 tttttgattt gttttaccgt acacagttgg gtgaaaattc attgggacgc cctgtcggcg
     2041 gtgagtctac acacatcgag cgcaccaaac gcgatactct tttcaattat tatcgcactc
     2101 actatggccc caatggtgca gtgctttcgt tggccggtgc cctttttaac tcagcgcaaa
     2161 gtaagaaatc cctcatcgcg acgatcgtgc gctttttcaa ccgcaacaat catacattgg
     2221 cgcccaagat cggctataag accgaggcgt atttaaagcc agaattccaa gaaaagcgta
     2281 ctgtcaaacc aaagagcttc aagcacttgc ctaaaaagtt agagcaagtt aactttgttc
     2341 tctcgctacc aggtataaaa aacgacatca aacctaccgc cgaaatgtcc gtgctcacgc
     2401 attacttagg cggcaccatg tcatcgcgcc tttttacaga gatgcgcgaa aaaaaagggc
     2461 tctgctattc ggtgagcgca ttccattcgc aatacttgca cgagggcatt tgggggattt
     2521 tttgtgcgac ttcacccaaa aaatacgccg acgcagtaga gaccgccgtt gctgaaatta
     2581 ctaaattggc cgatggtata tcgaagcatg aaatcgaaga aagtaaatcg gggttgcgcg
     2641 gcggcaccga actctcgatg gagagtgcgc accgtcgtgc ctctttcaat gcccgttcag
     2701 agctttatta cgacacgcag atatactggc atgaatatat cgctgccatc gatcgcgtca
     2761 cacccgaatc gcttcaacac acactcttga atctttggcg tagcgttgcg ccagggctca
     2821 caagcattgg gcctaaaagt tcttcgact
//
LOCUS       DAOMPD010000346         2843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14227_length_2843_cov_2.782640, whole genome shotgun sequence.
ACCESSION   DAOMPD010000346 DAOMPD010000000
VERSION     DAOMPD010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14227_length_2843_cov_2.782640"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLY93_11080"
     CDS             complement(<1..361)
                     /locus_tag="PLY93_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRP70065.1"
                     /translation="MLQQRSIGLIASTSIVAGCMIGTGIFLKPATMASEAGSITIVLL
                     AWLIAGLLSYAGALTYGELCAHFPAAGGEYAILRESYGRFPAYMYGWMRFTIGAPGSA
                     ASYAVGAATFLHVVIPYE"
     gene            complement(366..1250)
                     /locus_tag="PLY93_11085"
     CDS             complement(366..1250)
                     /locus_tag="PLY93_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70066.1"
                     /translation="MAVHALLYRADVPDSRVDRWNIKLIERIVKKSTTPAIAEIYYYQ
                     STQISSWVVPHENVRFHRLRSGEAFPLAERINQTHADYVLVFDNSTYLSAADIWLLMG
                     LVKMQSVVVVNPTRFAARKNENFWVRQLRHARFALHTYLKRPTPAMGIFFAVDKTKMR
                     QTTNFLQKITYADFIRATYKNAPQQIQGQSRSAAKGTQHDFLETVASTVEALQIWKRD
                     KSFPYWFSHRFLLFIVAQLAVYIGVLAFYIAPLYSLPFFLFAIFITPQFFFAHFSWRS
                     PCHALVQGCTRLLLYLIG"
     gene            complement(1275..2165)
                     /locus_tag="PLY93_11090"
     CDS             complement(1275..2165)
                     /locus_tag="PLY93_11090"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRP70067.1"
                     /translation="MFRRLKPLFFLLPLVLVACTYKEGMLKSTIVSWAQVSPYGITMA
                     LAFYSSFLLIEREFPRVGLDVKLGDYILLAGVLGGIIGSKIAYVIEIRNTPEFLQGSL
                     WSHLFSGAGLTWYGGFILASIAIIYILYKNKVNILLGMDILTPMLALGYVFGRAGCIF
                     SGDGCYGGLCTWNLPAPFCMAFPNGAASWSQWLGEHANGNLAATAINTPFFESAFSFL
                     CFIFLWNVRKKNWPLGSKFFLYLFLHALFRFSIEFIRLNPRDVFGVSQAQFLSLILMA
                     ISLGYVLIAFKRHGRLEKTG"
     gene            2262..>2843
                     /locus_tag="PLY93_11095"
     CDS             2262..>2843
                     /locus_tag="PLY93_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP70068.1"
                     /translation="MAFTILSVDDHPVIFIGVQAALEKDGFAQIIQCTDYRRIASFID
                     KYAPEIVLLDLYMPKFAIERDVPHLCRKHPNTFFVAYTAEEDVQMIQRCKKLGFVGYI
                     IKSQSLTVIRKEIEAISRGELVFPAAPLLSENAAVAMPLTETQMEVLRLMASGHKNAE
                     IAQLLNMRESNVDYHKRNIKKILNANTSSEVITI"
BASE COUNT          852 a          627 c          717 g          647 t
ORIGIN      
        1 tttcataagg gataacgaca tgtaaaaaag tagcggcgcc gacggcgtac gatgctgcgc
       61 tgccgggtgc accgatagtg aaacgcatcc aaccgtacat gtacgcaggg aagcggccat
      121 agctttcacg gaggatggcg tattctcccc ctgcggcggg gaagtgtgcg caaagctcgc
      181 cataagtgag cgcaccggcg taagagagaa gaccggcgat aagccatgcg aggagcacga
      241 tggtgatact ccccgcttcg gaggccatcg ttgctggttt caagaagata ccggtgccaa
      301 tcatacaacc agcgacgatg ctggtggagg cgataagacc gatcgatcgc tgttgtaaca
      361 tgtgtctatc ctataagata gagcaaaagc cgagtgcagc cttgcacgag cgcgtggcag
      421 ggagagcgcc atgaaaagtg cgcgaaaaag aattgtggtg taataaaaat tgcaaacagg
      481 aaaaaaggga gactgtaaag cggggcgata taaaatgcca acacgccgat gtaaacggca
      541 agttgagcga ctataaaaag taaaaaacgg tgtgaaaacc aatacggaaa acttttatcg
      601 cgtttccata tttgtaacgc ttcaacggtc gacgcaacag tttccaaaaa atcatgctga
      661 gttccttttg ccgccgaacg actttgccct tggatttgtt gcggcgcatt cttgtacgtg
      721 gcgcggatga aatctgcata ggttattttt tgcagaaagt ttgtcgtctg tcgcattttt
      781 gttttgtcga cggcgaagaa aatgcccatt gcaggcgtgg ggcgcttgag gtatgtgtgg
      841 agtgcaaagc gggcgtggcg tagttgacgc acccaaaaat tttcattttt gcgcgcggca
      901 aagcgagtgg gatttacaac gacaacgctt tgcattttga cgagtcccat caaaagccaa
      961 atatcggcag cggaaagata agtggagtta tcaaaaacta aaacatagtc tgcgtgggtt
     1021 tgattaatgc gctctgccag tggaaacgcc tcaccgctgc gcaagcggtg gaagcgtacg
     1081 ttctcatggg gcacaaccca cgaagaaatt tgcgtgcttt ggtagtaata aatttcagca
     1141 atggcggggg tggtactctt ttttacaatg cgctcaatga gttttatatt ccatcgatcg
     1201 acgcgcgaat cggggacatc cgcgcggtaa agaagagcat gcactgccac aacttattgg
     1261 atacgcctta aagcttaacc ggtcttctcg agtctgccgt ggcgcttaaa cgcgatgaga
     1321 acgtagccca gcgatatagc catcagaatg agcgacaaaa attgcgcctg tgaaacgcca
     1381 aacacatcgc gggggttcaa gcggataaat tcaatcgaaa agcggaagag cgcgtgtaag
     1441 aaaagataga gaaaaaactt agagcccaat ggccaatttt tcttacgcac attccacaag
     1501 aaaataaaac agaggaatga aaatgccgat tcaaagaaag gagtgttgat ggcggtggcg
     1561 gctaaattgc cgttcgcatg ttcgccgagc cattgactcc aactggcggc gccgttggga
     1621 aaagccatgc agaaaggcgc gggcaaattc caagtgcaaa gcccaccgta gcaaccgtcg
     1681 cccgaaaaaa tacaacccgc acgaccaaac acgtaaccta aagcaagcat tggggttaga
     1741 atatccatcc ccaaaagaat gttcacttta tttttgtaaa gtatgtagat gattgcaatg
     1801 gatgcaagaa taaatccacc gtaccaagtg aggccggcgc cggagaaaag atgcgaccac
     1861 agcgaacctt gtaaaaattc tggggtatta cggatttcaa taacgtatgc aattttagaa
     1921 ccgatgatgc ctcccaaaac ccctgcgagt aaaatataat cgccgagttt aacgtcgagc
     1981 cccactcggg gaaactcacg ctcgatgagg agaaaagaac tataaaaagc gagcgccatg
     2041 gtgatgccat aaggtgaaac ctgcgcccaa gaaacgatgg tgcttttgag cataccttct
     2101 ttataagtac acgcgacgag cacgaggggt aaaagaaaaa agagtggttt taatcgtcga
     2161 aacatacggc acagatggcg tgtaaaacgc gcggtcaatc gaatccgata ttacccaaaa
     2221 aatgtttgca aggcagcctt aaaagataaa agcacctgtt aatggccttt actatattga
     2281 gtgtcgatga ccatcctgtt atttttattg gggtgcaagc agcccttgag aaagatggtt
     2341 ttgcacaaat catccagtgt accgactatc gacgcatcgc ttcgtttatc gataagtatg
     2401 ctcccgaaat tgtgttactc gatttgtata tgcctaaatt tgccatcgaa cgcgatgtcc
     2461 cccacctttg tcgtaaacac cccaacacat tttttgttgc gtataccgct gaagaagatg
     2521 tgcaaatgat tcagcgttgc aaaaaactgg ggtttgtagg gtatataata aaaagtcaat
     2581 cgctgaccgt tattcgtaaa gagattgagg ccatttcgcg cggggaactg gtcttccctg
     2641 cagctccatt actttcggaa aatgccgctg ttgccatgcc tttgacggaa actcaaatgg
     2701 aggttttgcg tcttatggcc tcggggcata aaaacgcaga aattgcacaa ctcttaaaca
     2761 tgcgcgaatc taatgtcgat tatcacaaac gcaatataaa aaaaatactc aacgcgaata
     2821 cttcgtccga ggtcatcacg att
//
LOCUS       DAOMPD010000347         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14236_length_2842_cov_2.778615, whole genome shotgun sequence.
ACCESSION   DAOMPD010000347 DAOMPD010000000
VERSION     DAOMPD010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14236_length_2842_cov_2.778615"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(682..1449)
                     /locus_tag="PLY93_11100"
     CDS             complement(682..1449)
                     /locus_tag="PLY93_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70069.1"
                     /translation="MLRHLLILSLISTMGCEIKTCYQEAKEKHLEKRYGHKPVSKEQL
                     ADWEKEIEGYSKIINQKIDAGIKTGKVYRKLGESYALMESYDLCIQNLDKAAEYGEAV
                     AEVFYWQGLCYANKARVNNWQADYARKAEQAFLKALNLDKTMNKVKYELAVMYYAGFA
                     RNNQYRVLNDTLTVSQQQFQDKAIELMQEYKSVEPDQAKAYFFLAGLYKERGELAEAK
                     REMRDLMAFIEKNNSMSYKRNLDYQRAATYLQDFNKK"
     gene            complement(1466..1972)
                     /locus_tag="PLY93_11105"
     CDS             complement(1466..1972)
                     /locus_tag="PLY93_11105"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRP70070.1"
                     /translation="MDPPPPEPVVEEVAPEPELKAAKPEPEPIVAPSEIYEEVMEVAV
                     KPSLNLRTKPNTRGKILKRIPYGEAVTILDRFDDDTRTSSNQWYRVRYRGKIGYVNAY
                     YLRGEGEDQVIPEKRKSTTRSRYKVRKKSRVKKHRHNRIKHAAPKKAQKEESYDDGYN
                     ADAEPSPR"
     gene            complement(2074..2565)
                     /locus_tag="PLY93_11110"
     CDS             complement(2074..2565)
                     /locus_tag="PLY93_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70071.1"
                     /translation="MRQAVKVKLSSWCTLLSAFVLFACGSSRDNVFKIGIEEGWLDQT
                     RYVVLALGIQEYTNEPTKVAVEGACAIAEAKIPQRFSEIILGDTTKRCEGVDPTTDSL
                     CDWGYRLDRRIFFFRGVDIKRRHDFTSKKAICEVAHVYYHPALRYQMQQYAARFNLVP
                     AIE"
     gene            complement(2572..>2842)
                     /locus_tag="PLY93_11115"
     CDS             complement(2572..>2842)
                     /locus_tag="PLY93_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRP70072.1"
                     /translation="YVTGAAVFEATHGTAPKYAGLDKVNPSSVILSGEMMLRYMGWVE
                     AADSIIASLKKTISQKTVTYDFARLMDGAKEIKCSEFADALIKNL"
BASE COUNT          713 a          661 c          643 g          825 t
ORIGIN      
        1 tgaagcggcc aaaaacgtga atatcgctgt gcctttaatt gtgcgtctgg cgggcacgaa
       61 cgccgaggaa gcgaaagaaa tcttaaagaa aagtgggatt aaactcgaag tggccgatgg
      121 cctcaaagac ggcgccgaga aagtcgtaaa agcgattgct taattaagtc ttgaattatg
      181 tgcgcggatg cgcacataat tcaagtaaac tagattagcg gcagacctta acgttagacg
      241 ctgttgagca gctgctggtg cctgtgattt tacagccttt cttctcgcat tttttacgtg
      301 cttcaaacga atctgagcta cccaagctac attggttgca agcagatccc gaagcgcagt
      361 ttgcgcattt ttctgcatag atagacgtgc cgagtgcagg tacaacgaac aacaaacaaa
      421 caaaaatcaa ttttttcatg tgaactcctt tgaggtaagg ttgtcctaaa aatcataaat
      481 ctgatgtttt gcgcgcgtag tgcgtcaaaa atatcaataa atttaatatt cttgaggaac
      541 taatggttac gccaacagac cattagacac ccctccccta agccacttta gaatggcgaa
      601 tgaatgttac aactttttta tcaagaaata caaatgttgc aataattttc caatacttac
      661 ttttttagct ttttacaata gttacttctt attaaaatcc tgtaaatacg tggccgcgcg
      721 ttggtaatcc aaattgcgtt tgtaactcat tgaattattt ttttcgataa acgccatcaa
      781 atcacgcatt tcacgcttgg cttcggcaag ctcaccgcgt tctttataaa gaccggcgag
      841 aaaaaaatac gcctttgctt gatcgggttc caccgattta tactcttgca tgagttcaat
      901 cgctttatct tgaaattgct gctgagaaac ggtaagcgtg tcgttcaaaa cacggtattg
      961 gttattgcgc gcaaaaccag cgtagtacat cacggctagc tcgtacttga ctttgttcat
     1021 cgtcttgtcg aggttcagcg ctttcaagaa agcttgctcc gctttccgtg catagtccgc
     1081 ctgccaatta ttcacgcgcg ctttgttggc gtagcacaaa ccttgccaat agaacacctc
     1141 ggcgacggct tcaccatact cggcggcttt atctaggttt tgaatgcaaa gatcataact
     1201 ttccatgagc gcgtacgatt cacccagttt gcgatagact ttgccggtct tgatgcctgc
     1261 atcaattttc tgattgataa tttttgaata gccttcgatt tctttttccc aatcggcgag
     1321 ctgctcttta cttacaggtt tatgcccata acgcttctct aaatgttttt cttttgcttc
     1381 ttgataacaa gttttaatct cacagcccat cgtgctgatg agcgacaata tgagtaggtg
     1441 gcgtaacatc gctgcttatc tcgctttaac gtggcgacgg ctctgcgtcg gcgttatacc
     1501 cgtcgtcgta gctttcttct ttctgcgctt ttttaggcgc agcgtgtttt atgcgattgt
     1561 ggcggtgttt ttttactcta ctttttttgc gaactttata acgcgaacgc gttgtgctct
     1621 tacgcttttc tggtataact tgatcttcgc cctctccgcg caaatagtat gcgttgacat
     1681 agccgatttt tccacggtag cgcacgcggt accattggtt tgacgaggtg cgtgtgtcgt
     1741 cgtcaaagcg atcaagaatt gtaaccgctt cgccataagg aatacgcttc aaaattttgc
     1801 cgcgcgtgtt tggtttggtg cgcaagttga gcgatggctt gacggcgacc tccatcacct
     1861 cttcgtaaat ttctgacggg gcgacgatgg gttcgggttc gggtttcgcg gctttaagtt
     1921 cgggctcggg cgcaacctct tccacaacgg gttcgggtgg gggtgggtcc acttttttcg
     1981 gggcggattt acactgactt agaaacaatg ccagtgttgc ggcgagaaag cacttgccac
     2041 aaaaaacaat tttcagcatg atttattatc ggcttattcg attgcgggga caaggttaaa
     2101 acgcgcggca tactgttgca tttggtagcg gagcgcaggg tgataataaa cgtgcgcaac
     2161 ttcacaaatt gcttttttag aagtaaagtc gtggcggcgt tttatatcaa caccacgaaa
     2221 gaaaaaaatc cgtctgtcta aacgataacc ccaatcgcaa agcgaatcgg ttgtgggatc
     2281 gacaccctcg cagcgttttg tagtgtcgcc taaaataatt tcggaaaatc gctgcggtat
     2341 ttttgcctcg gcgattgcac acgcaccttc gacggcaact ttggtgggtt cgttggtgta
     2401 ttcttggata cccaacgcga gcaccacata acgagtttgg tcgagccacc cttcttcgat
     2461 accaattttg aaaacattat cgcgcgatga accacacgca aaaagaacga aagcgctcaa
     2521 aagtgtgcac caagaggaga gttttacttt cacagcttgg cgcactatga attataaatt
     2581 tttaatcaac gcgtcggcaa actcggaaca cttaatctct ttcgcgccgt ccatgaggcg
     2641 tgcaaaatcg tacgtgaccg ttttctgcga aattgtcttt ttaagcgagg caataatact
     2701 gtcggcagcc tcgacccagc ccatatagcg cagcatcatt tcgcccgaaa gtatcaccga
     2761 cgacgggttc actttatcta agcccgcata tttcggcgcg gtgccatgcg tcgcttcaaa
     2821 aacagccgcg cccgtcacgt ag
//
LOCUS       DAOMPD010000348         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14239_length_2842_cov_2.432365, whole genome shotgun sequence.
ACCESSION   DAOMPD010000348 DAOMPD010000000
VERSION     DAOMPD010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14239_length_2842_cov_2.432365"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>842
                     /locus_tag="PLY93_11120"
     CDS             <1..>842
                     /locus_tag="PLY93_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HRP70073.1"
                     /translation="TIDEGTGGFTERIKRRVVVPYLGSLDEYRHVVVHELVHAFQYDI
                     LLADSIGSAFTLVRTGEPPLWFIEGMAEYFSLGWDTTVDMQMRDAALTDTLPTLKMMT
                     EYRVPNPFIFYKGGQSVMRYIADTYGEHKLGEILRDMRDLRGIDDAIKTNLGIPFDEF
                     DASWRRWVKRLYFPQVDKKYDNEDGPLVSRHNDDESMINLHAAISPDGTKLVYTTARG
                     FYPVLILRDIKDVSLAKDYSLKKKSDIADSDIVLLTSSDNPTFYQLHLLTNRVSFTPD
                     GKRI"
     assembly_gap    843..857
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <858..2667
                     /locus_tag="PLY93_11125"
     CDS             <858..2667
                     /locus_tag="PLY93_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRP70074.1"
                     /translation="QGKDRLILFDIQKRRVVKTWAPAVDMVISPRLSSDGKYAIFVGA
                     TLGVTDIYLFELKTGKTHKITDDNFSEKDPTLSGDNKFVAYSSNKNSENNWASATYHL
                     YEKNLATKEIRQLTFDAQKQLSAQFFDPTSNDTLLYVSNHTGIQNIYLLNRTTLEKKK
                     LTDAAGGALEAQVSAGHVLNTANTRRIVYTQFREQGFDIALRDVDLAHENFYPTDTVV
                     QTYQPFTLPPYNTNAKPTSLTPYSPRLTPDFLFFLLGYGAYESGSYFAGMVAVNGSDL
                     TGNHSFSVVANFLTNGLGIFNFDYGYLKRRLKMHWGVYRTTFAISPVNLIDLQSLNGL
                     FWYPNYVASVMKFGAYFGMDYPLTPFDSLLLQFNVARTEETFIFDYTTPYSRYQPDRF
                     TNLYSVQYGFRHNNVLYSIIGPLKGWHFAYVGEQTFNLSGHDYVFNRNTVEARFYWNI
                     YQRYIFASRAYAATTNGPDAVYFPWIIGGPFNLRAYPFWSLRGESAFFFNLEFRFPLL
                     DALLFGFPVPWVLQGFSMVVFLDGGGVFDNPKTWRGYNTAEGKLDDFLLSYGLGVRFI
                     LVPGLMLKIDWGTPWNLRTARPIGDWQGAFSIGYEY"
BASE COUNT          698 a          712 c          653 g          764 t
ORIGIN      
        1 tgaccatcga cgaaggcacc ggtgggttca ccgagcgcat caaacgtcgc gtggtggttc
       61 cttatttagg cagtctcgac gaataccgtc acgttgtggt gcacgagcta gtgcatgcat
      121 tccaatacga catcctcctt gccgattcca tcggcagtgc gtttactttg gtgcgcacgg
      181 gcgagcctcc gctgtggttt atcgaaggta tggcggaata tttttcgttg ggttgggaca
      241 ccaccgtcga catgcaaatg cgcgatgccg cgctcaccga cacactccct acgctcaaaa
      301 tgatgaccga gtaccgcgta cccaacccgt ttatttttta caaaggcggt caatcggtga
      361 tgcgttatat cgccgacact tacggtgaac ataagctggg ggaaattttg cgcgatatgc
      421 gcgatttacg cggcatcgac gacgcgatca aaaccaactt agggatccct ttcgatgagt
      481 tcgacgcgtc gtggcggcgg tgggtgaagc ggctttattt tccgcaagtg gataaaaaat
      541 acgacaacga agatggtccg ctcgtcagtc gccacaacga cgatgagtcg atgattaact
      601 tgcacgcggc aatttcaccc gacggtacca aacttgttta taccactgcg cgagggttct
      661 atcccgtgct tattttgcgc gacattaaag acgtttcgct ggcgaaagat tattcgctaa
      721 aaaagaaaag cgacatcgcc gattcggata tcgttctttt aacaagctcg gacaatccca
      781 ctttttatca actgcactta ctcactaacc gtgtgtcgtt tacccccgac ggtaagcgga
      841 ttnnnnnnnn nnnnnnncca aggaaaagac aggctgattc tttttgacat ccaaaagcgg
      901 cgcgtcgtga aaacgtgggc acccgctgtt gatatggtta taagcccgcg tctctcctcc
      961 gacggtaaat atgccatctt tgtgggagca acactggggg taaccgatat ttatttgttt
     1021 gaactgaaaa ccggaaagac acacaaaatt accgacgata atttttcaga aaaagacccc
     1081 acgctttccg gcgataataa gtttgttgcg tattcttcaa ataaaaacag tgaaaataat
     1141 tgggcgagcg cgacatacca cctctacgaa aaaaatttag cgacgaaaga aatccgccag
     1201 ttaacgtttg atgcacaaaa gcaactctct gctcaatttt ttgatcccac atcgaacgac
     1261 actcttcttt acgtatcgaa ccacaccggc atccaaaaca tttatttatt gaatcgcacg
     1321 actctcgaaa agaaaaaact caccgatgca gccgggggcg cactcgaagc gcaagtgagc
     1381 gcggggcatg tgctgaatac cgccaacacc cgtcgcatcg tttacacaca attccgcgag
     1441 caaggctttg atattgcgct gcgcgatgtg gatttagcgc acgagaattt ttatcccacc
     1501 gacaccgtcg tccaaacgta tcaacctttc acgctccccc cttataacac caacgcaaag
     1561 ccaacttctt tgacgccgta ttcgccacgc ctcacacccg attttttatt ttttctttta
     1621 gggtatggcg cgtacgaaag cggcagctac tttgccggaa tggttgcggt gaatggctct
     1681 gacttaactg gcaaccacag ctttagcgtg gtcgccaact ttttgacaaa cggcttgggg
     1741 attttcaatt tcgattacgg ctacttgaag cggcggctca aaatgcactg gggagtatac
     1801 cgcaccactt ttgccatctc accggtaaac cttatcgatt tgcaatcgct caacggtctt
     1861 ttttggtacc ccaactacgt ggcgtcggtg atgaagtttg gcgcctactt tgggatggat
     1921 tacccgctga cgccgtttga ttcgttgttg ttgcaattca atgtcgcacg caccgaagaa
     1981 acttttattt tcgattatac aactccctat tctcgttatc aacccgaccg cttcactaac
     2041 ttgtactcgg tgcagtatgg ctttagacac aataacgtcc tctactcgat tattggtccg
     2101 ctcaagggct ggcattttgc gtacgtgggg gagcaaacgt tcaatctatc gggacacgat
     2161 tatgtcttca accgcaacac cgtcgaagcg cgcttttatt ggaatattta ccagcgttat
     2221 attttcgcca gccgcgcata cgccgccaca accaatggcc ccgatgcggt atattttccg
     2281 tggattatcg gcggtccctt caatttacgc gcctatccat tttggtcgct ccgtggcgaa
     2341 agcgcattct tctttaattt agagttccgc tttccgctgt tggatgcact cctttttggt
     2401 tttccagtac catgggtttt gcaaggtttc tcgatggtcg tgttcttgga cggtggtggt
     2461 gtattcgata atcccaaaac atggcgtggt tacaacactg ctgaaggtaa gctcgatgat
     2521 tttttacttt cgtatggatt gggcgtgcgc tttattctcg taccaggact tatgttaaaa
     2581 atcgattggg gtacgccgtg gaatttacgc actgcgcgcc ctatcggcga ttggcagggt
     2641 gcatttagta tagggtatga gtattaaaat cccgaattat tttttacgaa agaaaaattc
     2701 gtaaacggta attattgcaa tagtcgcact gacaaccatt gtgatactca tatcacgaac
     2761 ctatcgtaat gcctcggacg cgtcaacttt ttgtctaacc cacggacata cctgacaaat
     2821 gctacgccgc aattgggggg ta
//
LOCUS       DAOMPD010000349         2834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14289_length_2834_cov_3.195394, whole genome shotgun sequence.
ACCESSION   DAOMPD010000349 DAOMPD010000000
VERSION     DAOMPD010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14289_length_2834_cov_3.195394"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PLY93_11130"
     CDS             <1..940
                     /locus_tag="PLY93_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRP70075.1"
                     /translation="AVLHGQMADSAIEEVMVDFYQHAYDILVSTTIIESGIDIPNCNT
                     LVVLSAHEFGLSQLYQLKGRVGRSEKQAYAYFFYPPGQVKSLNGDAERRLEVLAEYDD
                     LGSGFKIAMKDLEIRGAGNLLGKEQSGDIMEIGFELYSKMLSDKIAELKGKAEAAEFE
                     TTITIPYDWFFPDDYIADTREKMEFYKTFSSAQSMEEFTLAKENLIDRFGTPPAVVER
                     MLNFEEIRQIANSLRIERIALGGSSDDENKGVHLIVAADHLLDMNKVTTLLTRDKRVS
                     LDRLDAKRINLDIALTPQIAFFRELRALLKSLSSFA"
     gene            942..2126
                     /locus_tag="PLY93_11135"
     CDS             942..2126
                     /locus_tag="PLY93_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRP70076.1"
                     /translation="MKKGFVEKIIERDTYASLAKLIRAHLDKKFAVDELLVQDALGVW
                     HSSNENKLYVQIRNFRIVNKKLRVAFHSGNKIREIKKYLKEIYPPIYLKLESIDLREK
                     MVRFTLLTQSEDKFSRHRFQKAQENARRQFEKKLNLFGKYSHDLKTPFSTLITSVENM
                     VLSDEPVPAKLRMRLDTIRTAIYSTLRTAGQSLDAARLFTRRRKTILVPYNFSQFVKQ
                     LTEFYEIIFESYGLTLSTEIHPNIPVEIDPIQMEKVLNNLLNNAVKHNIPGGFAHVAL
                     FTQKEKVVLKISDTGLGHVPNVEKIKDRNPWNFSSHGYGLEIVRELVKINRGRLHFEN
                     KQGVGTTVTVSLPHVSKLEGVVLALRGHNFQTTMHEVELLANERIRLSRKPTLKKPIP
                     KR"
     gene            2166..2621
                     /locus_tag="PLY93_11140"
     CDS             2166..2621
                     /locus_tag="PLY93_11140"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRP70077.1"
                     /translation="MSEILYGGFWRRLVAAILDQIILLLGRAFIYGVLTLIVYASLYL
                     FEVKYYHVIIYSVFGFFIFVLNIWLTWIYFALMESSSLQGTLGKLALGVRVYHIENRP
                     LTFEEATKRYFAKILSRYILLIGYILAAFSSKKQALHDFIANSVVLVER"
     gene            complement(2625..>2834)
                     /locus_tag="PLY93_11145"
     CDS             complement(2625..>2834)
                     /locus_tag="PLY93_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70078.1"
                     /translation="DDLNDVLIVGHNPVLEDLLTLLTARVPFHTKMPTCALAGVHFHV
                     SLWSEIRPATGILRVLLYPKLFAES"
BASE COUNT          845 a          559 c          654 g          776 t
ORIGIN      
        1 tgcggtgtta cacgggcaaa tggcagatag tgcgattgaa gaagtgatgg tcgatttcta
       61 tcaacacgcg tacgacattt tagtgagcac aacaatcatc gaatcgggga ttgatattcc
      121 aaattgtaat acactcgtag tgctatcagc acatgaattt gggttgagtc aactctacca
      181 actcaagggt agggtggggc gttcggaaaa acaagcgtac gcgtactttt tttatccccc
      241 agggcaagta aaatcactca acggcgatgc ggaaaggcgg ttggaagtgc tcgctgaata
      301 cgacgatttg ggttctggtt ttaagattgc gatgaaggat ctagaaatcc gtggcgccgg
      361 taatctcttg ggaaaagagc agagcggcga tattatggaa attggttttg agctctattc
      421 caaaatgctg tcggataaaa tagcagaact caaaggcaaa gcagaggccg ctgaatttga
      481 aaccacgatc accataccct acgattggtt tttccccgac gactatattg ccgatacgcg
      541 cgaaaagatg gagttctaca aaacgttttc atcggcgcaa tcgatggaag agttcactct
      601 tgccaaagaa aatttaatcg atcgctttgg tactcctcct gcggtagtgg agaggatgct
      661 caactttgaa gaaattcgac aaatcgccaa tagcttgcgg atcgagcgca tcgcgttggg
      721 gggaagtagc gatgacgaga acaaaggagt gcatcttatt gtcgccgcag atcacctttt
      781 ggacatgaat aaagtgacga cgcttttgac gcgcgacaaa cgagtctctt tggatagatt
      841 agatgcgaaa cgcataaatc ttgacattgc attaacgcca caaattgcat tttttagaga
      901 gctgagagcg ttattaaaat cgctgtcgag ttttgcttaa aatgaaaaaa ggatttgttg
      961 aaaagattat cgagagagat acatacgcct cgttggcaaa acttatccga gcgcatttag
     1021 ataaaaaatt cgctgttgat gaactgctcg ttcaagacgc tttaggcgta tggcacagct
     1081 caaacgaaaa taaactctat gtgcagataa gaaattttag aatcgtcaac aaaaaattaa
     1141 gagtagcgtt tcactcggga aataaaattc gtgaaataaa aaaatactta aaagaaattt
     1201 atccaccgat ttatctcaaa ctcgaatcga tcgatttgcg agaaaaaatg gtacgcttta
     1261 cgctactcac ccaaagcgaa gataaatttt cgcgccaccg tttccagaaa gctcaagaaa
     1321 acgcgcgtcg ccaatttgaa aaaaaattaa atctttttgg taagtattcg cacgatttaa
     1381 aaactccttt ttccacgctc atcacaagcg tggagaatat ggtgttgagc gacgagcccg
     1441 tgccggcaaa actgcgtatg cggttggata cgatacgtac cgcaatttat tcgacactgc
     1501 gtactgcagg ccagtcgctc gacgctgcgc gccttttcac gcggcggcgt aaaactatcc
     1561 tcgtgccgta caatttttcg cagtttgtta aacagctcac cgagttctat gaaattattt
     1621 ttgaaagtta tgggttaacg ttaagtaccg aaatccaccc caatataccg gttgaaatcg
     1681 accccataca aatggaaaaa gtgttgaata atcttttgaa taacgcggtg aaacacaata
     1741 taccgggcgg ttttgcgcac gttgctcttt ttactcaaaa agaaaaagtc gtgctcaaaa
     1801 tttcagatac agggttaggc catgttccga atgtagaaaa aattaaggat cgtaatccgt
     1861 ggaatttctc ctcgcacggt tatggtttag agattgtgcg cgaattggta aaaataaatc
     1921 gtggccgttt acattttgaa aataagcaag gagtgggcac gacagtgacg gtaagcttgc
     1981 cccatgtttc taaattggaa ggcgttgtgc tggcgttgcg cggccataat tttcaaacca
     2041 caatgcacga ggttgaactt ttggcaaacg aacgcattcg cctttcgcgt aaaccaacgc
     2101 tcaaaaagcc catacccaaa cgataaattg gtcaaaaaaa ctccaaaaag cttcgatata
     2161 taattatgag cgaaattctc tatggtggtt tttggcgtag acttgtcgcg gccattctgg
     2221 atcaaattat tttattgctg ggtcgcgcat tcatttatgg cgttttaacg ctcatcgttt
     2281 atgcaagtct ctatttattc gaggtgaaat attatcacgt tattatttat agcgtttttg
     2341 gattttttat ttttgtattg aatatttggc taacatggat ttattttgcg cttatggaaa
     2401 gttcatcgct ccaaggcaca ttggggaaat tggcattagg cgttcgagtt taccatattg
     2461 aaaaccgccc gctcacattt gaagaagcga ctaagcggta ttttgcaaaa attttgagtc
     2521 gttatatttt gcttatcggt tatattcttg ccgcgtttag tagcaagaaa caagcgttac
     2581 acgattttat cgctaactcg gttgtgcttg tcgagcgtta agatttaaga ttctgcaaaa
     2641 agtttggggt agagtaacac acgcaaaatc cccgtagcgg gtcgaatttc agaccaaagc
     2701 gaaacgtgga aatggactcc cgctaaagca caggtcggca ttttggtgtg gaatggcacg
     2761 cgcgcggtga ggagcgtcaa gagatcttcc aacacggggt tatgccccac gatgagaacg
     2821 tcgttaagat cgtc
//
LOCUS       DAOMPD010000350         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14306_length_2830_cov_2.619820, whole genome shotgun sequence.
ACCESSION   DAOMPD010000350 DAOMPD010000000
VERSION     DAOMPD010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14306_length_2830_cov_2.619820"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1399)
                     /locus_tag="PLY93_11150"
     CDS             complement(146..1399)
                     /locus_tag="PLY93_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRP70079.1"
                     /translation="MAQHGKVYILGGYQTDFARNWSKEGKTIQALMNEAMDGAFAATQ
                     IDPADVESIHVGNFAGELYAMQGHLGAMSVNYSEKLRGVPTSRHEAACASGAISALMA
                     RTEIEAGHYNLSMVVGVELMKSVDSKTGGDFLGTAAWYDKEAKGVQFPFPKLFGRLGT
                     VYEELYGLKYDHLAEISAVNYENAKHNPVAQTREWYMNKDHALTRGNFNMEVGGIIHI
                     SDCSQVTDGSAVVFLASEKYASEYAKKHNLKLESIPHIMGWGHSTAPILFDVKTEEAH
                     AAKKKGEYILPHTRKAILDAYKRAGIEGSKDLDLVETHDCFTTSEYAAIEHFGVTAPG
                     EAYKAIESGDIKIGGKLPFNPSGGLIGAGHPVGATGTRQLLDVYKQLTQNAGANQVEG
                     AKVAATLNIGGSATTNVVHIVGTGK"
     gene            complement(1446..>1995)
                     /locus_tag="PLY93_11155"
     CDS             complement(1446..>1995)
                     /locus_tag="PLY93_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70080.1"
                     /translation="ERIDTYKMPAYMQKMIDQGMLGRKAKGKGGYFNRDAEKNKLTLD
                     IPSFSFNPTKKEKVDWVEKTKAAIHDGLYSQAINLVKTASGEEADVVRHFILGYVAYS
                     FARIGEVTPEKDGIHGIDRVMSSGFSWLPASGWVDLFGGVKETCALIEKAKLPVPQQL
                     SSIHTTGRLCQVADVTKFLAGR"
     assembly_gap    1996..2005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2006..2744)
                     /locus_tag="PLY93_11160"
     CDS             complement(<2006..2744)
                     /locus_tag="PLY93_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRP70081.1"
                     /translation="MTRTIKKVGVLGASGNMGSLSGGIFAQANIECVFFARTVDKAKA
                     GLEAAVNQARSDVLRKYIRAASYEELEKEIPTCDWIFEGLAEDMAIKNEFFSKIENLK
                     RADAIVSTVSSGLSIRKMAEGRNENFRKNFMGTHFYNPPGKLPANELIYHPDVPNETR
                     EFVYDFCEKVLRRVNIITEDTAAFAGNRIGFQFLNEAAIYAEKYGVDKMDYLLGPYTG
                     RALAPLATIDLVGLDAHKAIVDNVVANV"
BASE COUNT          620 a          723 c          697 g          780 t
ORIGIN      
        1 aattagccac ctggtctgcg tccattttgt cggtcgagat attgttatta tctaacgctt
       61 tatctgccaa aaatttctca aacttggtgt agaaattttt aacgtgcgct gtgagtgctt
      121 ttgccgtatc taacgccatt gtcgtttatt ttcctgtacc gacgatgtgt accacgttgg
      181 tggtggcaga tccgccgata ttcaaagtcg ccgccacttt cgcgccttcg acttggttgg
      241 cgccggcgtt ttgagtgagc tgcttataca cgtccaaaag ctggcgtgta ccagtagctc
      301 cgacaggatg gccagcaccg atgagaccac ccgatgggtt gaacggtaac ttaccgccaa
      361 ttttaatgtc gcccgattcg atggctttgt acgcttcgcc aggagcagtt acgccaaagt
      421 gttcaatcgc cgcatattca gaggtggtga agcagtcgtg cgtttctacg agatctaaat
      481 cttttgaacc ttcgatgcct gcgcgtttgt acgcatcgag aatcgctttg cgggtgtgtg
      541 gcagaatata ctcgcctttt ttcttcgctg cgtgcgcttc ttcggttttc acgtcgaaga
      601 gaatcggcgc agtcgagtga ccccagccca tgatgtgtgg gatactctcg agtttcaaat
      661 tgtgcttttt tgcgtattcg ctcgcgtatt tttcagacgc taagaacacc accgccgaac
      721 cgtcggtgac ttgcgagcag tcggagatgt ggatgatacc accgacttcc atattaaaat
      781 tgccacgggt gagcgcgtga tctttgttca tgtaccactc gcgggtttgc gccacagggt
      841 tgtgcttggc attctcgtag ttcaccgctg agatttcagc gaggtggtcg tatttcagtc
      901 cataaagctc ttcgtacacc gttcctaaac gcccaaagag tttgggaaaa ggaaactgca
      961 cgcccttggc ttctttgtcg taccaagccg cagtgcctaa aaagtcacca ccagtctttg
     1021 aatcgaccga tttcataagc tccacgccca cgaccatcga caggttgtag tggcccgctt
     1081 cgatttccgt acgcgccatg agagcagaaa tagcgcccga tgcacacgct gcttcgtgac
     1141 ggcttgtcgg cacaccgcgt agtttttcgc tatagttgac gctcatcgcg cccaagtggc
     1201 cttgcattgc ataaagctcg cccgcaaagt taccgacatg gatcgactct acatcggcag
     1261 ggtcgatttg tgtggcggcg aatgcgccgt ccattgcttc gttcatcaac gcttggatcg
     1321 tctttccttc tttagaccaa ttgcgcgcga agtcggtttg gtaaccgccc aaaatataaa
     1381 cttttccgtg ttgtgccatg tttatctcct taaagtcgtc ttttccaata taatcatcga
     1441 tacgcttaac gccctgctaa aaactttgtc acgtcagcga cttgacagag acggccagtg
     1501 gtgtggatcg acgaaagctg ctggggaacg ggtagttttg ctttttcaat gagagcgcac
     1561 gtttctttca cgccgccaaa aagatctacc caacccgagg caggtagcca cgaaaaaccc
     1621 gaagacatca ctcggtcgat gccgtggata ccgtcttttt caggggtcac ttcgccgatg
     1681 cgggcaaagc tatacgcaac gtagcctaaa atgaaatgac gcacgacatc tgcctcttcg
     1741 ccgctcgcgg ttttgacgag attgattgcc tgcgaataca gaccgtcgtg aatggcagct
     1801 ttggtttttt ccacccaatc gactttttct ttcttcgtgg gattaaagct gaaactgggt
     1861 atatccaacg tgagtttgtt tttctcagcg tcgcggttga aatacccgcc tttgcctttt
     1921 gccttgcggc caagcatccc ttggtcgatc atcttttgca tgtacgcggg cattttatac
     1981 gtgtcgatgc gttcgnnnnn nnnnntcacg ttcgcaacca cgttatcgac aatcgcttta
     2041 tgtgcatcga gcccgacaag gtcaatcgtg gcgagcggag cgagcgcacg gccggtgtat
     2101 ggccccagga ggtaatccat cttatcgacg ccatatttct cggcgtagat ggccgcttcg
     2161 ttcaaaaatt gaaaaccgat gcgattgccc gcaaacgctg cagtgtcttc ggtgatgatg
     2221 ttcactcggc gcaaaacttt ttcgcaaaag tcgtacacaa attcgcgcgt ttcgttggga
     2281 acatcggggt ggtaaatgag ttcgttcgcg ggcagttttc cgggcgggtt atagaagtga
     2341 gtgcccataa aattcttgcg gaagttttca ttgcgacctt cggccatttt acggatcgac
     2401 aaccccgatg aaacggtgct cacgattgcg tcggcgcgct ttaaattttc aattttggaa
     2461 aagaattcgt tcttaatcgc catgtcctcg gcgaggcctt caaaaatcca atcacaagtg
     2521 gggatttctt tttccaactc ttcgtaactg gcagcgcgga tgtatttgcg caacacatcg
     2581 gagcgtgctt ggtttaccgc tgcttcgagc cccgccttag ctttatcgac ggtgcgggca
     2641 aagaaaacgc attcaatatt agcctgcgca aaaatccccc cagaaagcga gcccatgttg
     2701 cccgatgcgc ctaaaacgcc gacttttttg atcgttcgtg tcatgtagtg tgtctcccca
     2761 cgtaatttaa gatatcagcg agcctttttg agtgttttag acagagcgac aacaaagttt
     2821 gtacagaaga
//
LOCUS       DAOMPD010000351         2816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14399_length_2816_cov_2.787396, whole genome shotgun sequence.
ACCESSION   DAOMPD010000351 DAOMPD010000000
VERSION     DAOMPD010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14399_length_2816_cov_2.787396"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLY93_11165"
     CDS             <1..435
                     /locus_tag="PLY93_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRP70082.1"
                     /translation="YMAYNRCIGTRYCSNNCPHKVRRFNWFENWENKLRDPQQYALNP
                     DVTVRSRGVIEKCSFCQQRISEKRQEAKVAGRAIKDREIQTACEQACPTDAIIFGNIN
                     SADTAVTKANSDPRSYELLAQINVKPSVKYRVRIKNRSEAKA"
     gene            437..1822
                     /gene="nrfD"
                     /locus_tag="PLY93_11170"
     CDS             437..1822
                     /gene="nrfD"
                     /locus_tag="PLY93_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRP70083.1"
                     /translation="MATEKNIYHIGKPLAAMDDDVMRPIEAFPTKLWWGAFGVSATLA
                     LIGVSTIIYSFIVGLGIFALNNPVGWGFMIVNFVFWVGIGHAGTLISAILYLFRQKWR
                     TSINRAAEAMTIFAVIVAAIFPLIHVGRPWFVFWTFPYPNERGPLWANFRSPLLWDLF
                     AIATYFSVSIAFWYMGLVPDFATLRDRFEKFKAKSKIGQFLLKVKAFAYRVLALGWVG
                     SAKTWTHYEKMVMLLSALATPLVLSVHTIVSFDFATSVIPGWHATIFPPYFVAGAIYS
                     GFAMVLTLMIITREVFKLKEYITVKHLENMAIVILVTGMMVSFAYTTEFVMAAYSASH
                     NEQYAFINRLKGPFAWAFWIMFTCNVVSPHFMWFKKLRTNIKALFAISIVVNIGMWFE
                     RFVIVVISLHRDYLPSSWDSYNPTWADYTILIGSFGIFFTLFLMFVRTIPTIALAEVK
                     ADETNSQLSHH"
     gene            1842..2390
                     /locus_tag="PLY93_11175"
     CDS             1842..2390
                     /locus_tag="PLY93_11175"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRP70084.1"
                     /translation="MLSIAEIKRGLFSYEEAKSGFIAVVKSAADLLKAAEKTKQANVK
                     SFDCFTPCPIHGLDTAMGIHRSWIPSLTLVGGILGAVLGLAYITFIDVFNWPIIYGGK
                     PYFSWPAYVPLLFELSIYFASFFTVFAVIALGKLWRINRKLPVQGVTSDVFAIWIGDA
                     NLSKADVERILSGLNVEIKEIQ"
     gene            2390..>2816
                     /locus_tag="PLY93_11180"
     CDS             2390..>2816
                     /locus_tag="PLY93_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70085.1"
                     /translation="MRNIFFLAAAFSFVACADVRREYHYFDDMHYSRASDTAKFDALG
                     NRLFDMLEPEHTITHEGKAAYPYRKDFDVDLATRELKAPAVTDTKRGAKEYHIYCEPC
                     HGADGLANGPVAKKFAGVRKLKNERADDFPITKIYHIATV"
BASE COUNT          698 a          675 c          636 g          807 t
ORIGIN      
        1 tacatggcgt acaaccgttg tattggtact cgttattgct cgaacaactg ccctcacaaa
       61 gtgcgtcgtt ttaactggtt tgaaaactgg gaaaacaaac tccgcgatcc tcagcagtat
      121 gcactcaacc ccgacgtaac cgttcgttca cgcggtgtga ttgagaagtg ttctttctgc
      181 cagcaacgga tttctgaaaa gcggcaagag gcaaaagttg cagggcgtgc gattaaagat
      241 cgtgaaatac aaaccgcatg cgagcaagca tgccccaccg atgcaattat ttttggtaac
      301 atcaatagcg cagacactgc agtgacaaaa gccaatagcg acccacggtc gtacgagctt
      361 ttagctcaaa ttaatgtaaa accatcggta aaataccgag tacgtattaa aaacagaagc
      421 gaggcgaagg cttagtatgg cgacagaaaa aaacatttac cacattggaa agccgctcgc
      481 ggcgatggac gacgatgtta tgcgtcccat cgaagctttc cctaccaaac tctggtgggg
      541 ggcttttggg gtttcagcaa cattggcatt gattggtgtc agtacgatta tttactcctt
      601 tatcgttggg ctcggcatct ttgccttgaa caaccctgtc ggttggggct ttatgattgt
      661 aaacttcgtc ttttgggtag ggattggcca cgcaggcacg ctcatttcgg cgattttata
      721 tttgttccga caaaagtggc gtacgtccat taaccgtgct gcggaagcga tgacgatttt
      781 cgcggtgatt gttgcggcga ttttcccgtt gatccacgtt ggtcgtcctt ggtttgtctt
      841 ttggacattc ccctacccca acgaacgcgg tccgctgtgg gcaaactttc gctcccccct
      901 tctttgggac ttgtttgcga ttgcgacgta cttctcggtc tcgattgcat tctggtacat
      961 gggactcgtc cccgactttg caacgctacg cgaccgtttt gaaaaattca aagccaaaag
     1021 taaaatcgga caatttttac tcaaagtaaa agcattcgca taccgtgttc tcgctttagg
     1081 ttgggtaggt tcggcaaaga catggacgca ctatgaaaaa atggtgatgc tcctttctgc
     1141 actcgccaca ccgctagtgc tctcggtaca caccatcgtt tcttttgact ttgcaacctc
     1201 ggttattccc ggttggcacg ccaccatttt ccctccttac tttgttgccg gtgcgattta
     1261 ttcgggtttt gcgatggttt tgacgctcat gattatcacg cgtgaagtct ttaaactcaa
     1321 agaatatatc acagtaaaac acttagagaa catggcgatt gtaattttgg tcaccggtat
     1381 gatggtgtct tttgcttaca cgacagaatt tgtgatggcc gcttactctg caagccataa
     1441 cgaacagtac gcgtttatca atcgtctcaa gggtcccttt gcttgggcgt tctggattat
     1501 gttcacttgt aacgtggttt ctccccattt tatgtggttc aagaaactgc ggacaaacat
     1561 taaagcctta tttgcaatct ctattgttgt aaatatcggt atgtggtttg aacgtttcgt
     1621 gattgttgtg atttcactac accgcgacta cttaccttca agttgggatt cttataatcc
     1681 tacttgggct gactacacga ttctcatcgg aagcttcggt attttcttta ccttgtttct
     1741 catgttcgta cgtacaatcc ccaccattgc attggctgag gttaaggccg atgaaaccaa
     1801 cagtcagttg agtcatcact aaggatcgtt aaagaggaaa tatgttatca atagcagaaa
     1861 ttaaacgcgg tctcttctcc tacgaagagg ccaagtccgg cttcatcgcg gttgtgaaat
     1921 ccgcagccga tctcttaaaa gcagcagaga aaacaaagca agctaacgtc aaatctttcg
     1981 attgcttcac cccctgcccc atccacggct tagacaccgc gatgggcata caccgttctt
     2041 ggattccatc actcacattg gttggcggta ttttaggcgc agttttaggg ttagcgtaca
     2101 tcacgtttat cgatgtgttt aactggccaa tcatttatgg tggaaagccc tatttctcat
     2161 ggcctgcgta cgtgccgctc ctttttgaac tttcgattta ctttgcttct ttctttaccg
     2221 tctttgccgt cattgcctta gggaaactat ggcggattaa ccgcaaactt ccagttcaag
     2281 gcgtcacctc tgacgttttt gccatctgga ttggcgacgc aaacttaagt aaagcagatg
     2341 tagagcgtat tctatcgggc ttaaacgtcg aaatcaagga gatccaataa tgcgaaacat
     2401 ttttttctta gcagcagcat tttcgttcgt tgcctgcgcg gacgtgcgcc gtgaatacca
     2461 ctatttcgac gacatgcatt actcgcgcgc ttcagacaca gctaagtttg atgcattggg
     2521 taatcgcctt ttcgatatgc tcgaacctga gcacaccatc actcacgaag gtaaagcagc
     2581 gtacccttac cgtaaagact ttgatgtcga tttagcaaca cgggagctta aagcgcctgc
     2641 tgttaccgac acgaaacgcg gtgcaaagga ataccacatc tattgcgagc cctgccacgg
     2701 tgcagatggt cttgcaaacg gtcctgtggc taaaaaattt gccggtgtac gtaagctcaa
     2761 aaacgaaaga gccgacgatt tccccataac taaaatctac cacattgcca cagtgg
//
LOCUS       DAOMPD010000352         2814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14412_length_2814_cov_2.936571, whole genome shotgun sequence.
ACCESSION   DAOMPD010000352 DAOMPD010000000
VERSION     DAOMPD010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14412_length_2814_cov_2.936571"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PLY93_11185"
     CDS             complement(<1..1358)
                     /locus_tag="PLY93_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRP70086.1"
                     /translation="MAYPFEEIEKKWQEAWEKNQTFKPEDDFSKPKYYVLDMFPYPSG
                     AGLHVGHPEGYTATDIVARAKRMQGFNVLHPMGWDAFGLPAERYAMQTGIHPAITTKK
                     NIDNFRRQLKALGFSYDWSREINTTDAGYYKFTQWIFLKLFNSFYDIEAKKARPIEEL
                     KIPQNLTGKEREDFIAEHRLAYIANMPVNWCAELGTVLANEEVDEWVGKGYKVERRPM
                     RQWMLRITTYAERLLDDLVHVEWPQSTLELQKNWIGRSTGALVTFNVSSVKTHHGLSL
                     QVFTTRPDTLYGVTFIVLAPEHPLVDTITPDSHKKAVAEYREKTALKTDLDRQRGVQL
                     SAQLDAGKKEKSGVFIGAYATHPLTQEKIPLYIADYVLMGYGTGAIMAVPAHDERDFA
                     FAGQFGLEIKPVVETPGSTGSPTDASLPRVEDGIAINSPLIDGLKTADAKIKIIEHLE
                     KE"
     gene            complement(1418..2515)
                     /locus_tag="PLY93_11190"
     CDS             complement(1418..2515)
                     /locus_tag="PLY93_11190"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP70087.1"
                     /translation="MDKPNDSPHTNSRKIIDPVQLHQITERLFCRLPIHLIHENQRVS
                     VKAFNYQYPHFEIQHHLPAIESRTLLLEKDDNSIMLECAIMRRTEHGTELVKPLRLYL
                     SKRGIRHEKRETLDDSLSFAGVVIHCLPHIEFYQLNAATNSGRDQLIQQYKNAIKGII
                     SNSLVKIELQRHNRFSVRMKKLIDFNLPIFAPMMEHERQGDNLSLPAVPFSEYKQVMR
                     ADGLPGDHMGEICEPLRYRGAFIIGFVQVLTHTPANLGQYHSVRQIVRRLENDLDKRG
                     AFPKNPITGKIIDVSPNGIGFNYTTQRNIMSSVSIGDKIVFDARFSPENVTTLSGKVM
                     NVSAQESAMRFGVELEHISEESQAAIKKMIS"
BASE COUNT          688 a          584 c          684 g          858 t
ORIGIN      
        1 ttttcttttt ctaagtgttc gattattttt attttggcgt cggcagtttt aaggccgtcg
       61 atgagaggcg agttgatggc aatcccgtct tcaacccgtg gtaaagacgc atcggtgggt
      121 gagcctgtcg aaccaggcgt ctctaccacg ggttttattt caagaccaaa ttgaccggcg
      181 aaagcaaagt cgcgttcgtc gtgcgcgggg actgccatga ttgcgccggt gccgtagccc
      241 atgagtacgt agtcggctat ataaagagga attttttctt gagtgagtgg gtgggttgca
      301 tatgcgccga taaacacccc cgatttttcc tttttgcccg cgtcgagttg tgcactaagt
      361 tgcacgccgc gttgtctatc taaatcggtt tttagcgctg ttttctcgcg gtattctgcc
      421 actgcctttt tatgcgaatc gggggtgatg gtatccacca gagggtgttc tggggccaac
      481 actatgaatg taacgccata gagggtgtcg gggcgtgtgg taaatacctg gagagacaaa
      541 ccatggtgcg tcttcacgga ggaaacgtta aacgtgacca atgcgccggt agagcggcca
      601 atccagtttt tttgtaattc caatgtgctc tgtggccatt cgacatgaac caaatcgtct
      661 aaaagacgtt cggcgtatgt ggtaatccgt agcatccatt ggcgcatcgg tcgccgctcg
      721 accttataac ccttacccac ccattcatcg acttcctcgt tcgcgagcac agtgcctagc
      781 tcggcgcacc agttaaccgg catattggcg atgtatgcta agcggtgttc agcgataaaa
      841 tcttcacgtt cttttccggt gaggttctgc ggtattttca gttcttcaat cgggcgggct
      901 ttctttgctt cgatgtcgta gaaagaatta aagagtttaa gaaaaatcca ttgcgtgaat
      961 ttataatagc ctgcgtcggt ggtgttgatc tcacgcgacc agtcgtacga aaaacccaac
     1021 gccttgagtt gacggcggaa gttatcaata ttttttttgg ttgtaattgc cggatggatt
     1081 cccgtttgca tcgcgtaacg ttcggcaggt aaaccaaaag catcccaccc catgggatgc
     1141 agcacgttaa acccctgcat ccgtttggcg cgcgcgacaa tgtcggtggc ggtgtatccc
     1201 tcggggtggc ctacgtgcaa ccccgcaccc gaagggtaag ggaacatatc gagtacataa
     1261 tacttgggtt tggaaaaatc atcttcgggt ttgaaggttt ggtttttctc ccatgcctct
     1321 tgccattttt tttcaatctc ttcaaagggg tatgccataa tgcagagttg acgtgcgaga
     1381 cagagtgaaa agcgttatgg aatagatgtt ttgactttta agaaatcatt tttttaattg
     1441 ccgcttgcga ctcttcggaa atatgttcga gttcaacgcc aaaacgcatc gcactttctt
     1501 gcgcactgac gttcatcact ttacctgaaa gtgtcgtcac attctcggga ctgaaacgcg
     1561 cgtcgaaaac aattttatcg cctattgaaa ccgatgacat tatgttgcgt tgcgttgtgt
     1621 agttgaagcc aatcccgttt ggacttacgt caataatttt accggtgatg gggtttttag
     1681 ggaatgctcc gcgtttatct aaatcgtttt caagacgacg tacaatctgc cgcacagaat
     1741 ggtattgccc caaatttgcg ggtgtgtgcg tgaggacttg tacaaaacca ataataaatg
     1801 caccacgata acgcaatggt tcgcaaattt cacccatgtg gtcgccgggt aatccatcgg
     1861 cgcgcatcac ttgcttgtat tcggagaaag gcactgctgg taaactgaga ttatcccctt
     1921 ggcgttcgtg ttccatcata ggagcaaaaa taggtaaatt aaaatcgatg agttttttca
     1981 tgcgcacgct gaagcggttg tgccgttgca gttcaatttt aaccaacgag ttagaaatta
     2041 ttcctttgat tgcattttta tattgctgaa tgagttggtc tctgcctgag tttgtggcgg
     2101 cgttgagttg ataaaactca atatgcggta aacaatgtat aacgacaccc gcaaaagaga
     2161 gcgagtcatc taatgtctct cgtttttcgt ggcggatacc acgtttggag agataaagac
     2221 gcagtggctt aacaagctct gtgccgtgtt cggtgcgtcg cataatagcg cattcgagca
     2281 taatggagtt gtcatctttt tccaaaagta aagttctgct ttctattgcc ggtaaatgat
     2341 gttgaatttc aaaatggggg tattgatagt taaacgcctt taccgagaca cgttggtttt
     2401 cgtggattaa gtgaatcggt aagcggcaaa aaagtcgttc ggtaatttgg tgcagttgca
     2461 cgggatcgat aatttttcgt gagttagtat ggggggaatc gttcggtttg tccatgagtc
     2521 agctattcac ttaacgaata aaaaatctct tactaaacgt aaatcttata taatggttcg
     2581 gtaaatggag tttgtataat gattcgtaaa attttaggat tccgcacaat tttaatggtt
     2641 atttctaagc ctccaaagtc gcaaaattga tttcggtatg catcggattg ttttcatcgt
     2701 catgtgacat aacgtttatt aactaacaac attcgatgtc tgctttaata tcttatgcat
     2761 tttttttgag tttttgcact ttttttttcc aaaagtgaga aaaatcgagc attt
//
LOCUS       DAOMPD010000353         2800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14489_length_2800_cov_3.105647, whole genome shotgun sequence.
ACCESSION   DAOMPD010000353 DAOMPD010000000
VERSION     DAOMPD010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14489_length_2800_cov_3.105647"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..725)
                     /locus_tag="PLY93_11195"
     CDS             complement(114..725)
                     /locus_tag="PLY93_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70088.1"
                     /translation="MPTEYPTPTNTNKKSTTQTIGILLIVFASMYLFYSLYAIGAALL
                     ASAFVDALPALKKIIPEFKNADVEVQIFVQPIKTLYHIQALEKVATMVISAYGLFAGI
                     RLVQLQQKKSGILHSIRWAYTALVYLLLELVIFLLVQQPLVRRLFQTISEKITPLVED
                     GAAGTEFLSGLANNSGMGSEIAGLLFLAAFPIVTILLLHRAEK"
     gene            960..1676
                     /locus_tag="PLY93_11200"
     CDS             960..1676
                     /locus_tag="PLY93_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HRP70089.1"
                     /translation="MQQQLRYIERFVDGIRVIEIPGAPDAPTFFCMHGYGANAFDLAG
                     LSQHLAFPSSTRYLFPDGILGVDAGFGNGRAWFPIDWVEIERIRREGGLRDLSKNLPL
                     GMAEAREKVIGLLTKLSVDIDKTVIGGFSQGAMMATEILLSHRLYFKGLILFSGTLVN
                     RVGWQQLLQKKFMEQNKKLQFIQSHGRFDPVLPFQAAEWLNQLLNDDGGRGELVAFDG
                     QHEIPASVVEATNRWLQSSL"
     gene            complement(1787..2215)
                     /locus_tag="PLY93_11205"
     CDS             complement(1787..2215)
                     /locus_tag="PLY93_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70090.1"
                     /translation="MLKTSISLPRKSIPTWLANRWAIMRMYTRFLRIVERKRKFRRGV
                     TRQYNKGNGHCGIVKVSFTAKEYDSLHTMAAGIRTSVSYLIYYLIQFWEKASEKKAKF
                     VVGGNYTPRVLEWSKVRVNFEEKLSFRRAAHRFFYHTCLF"
     gene            complement(2438..2719)
                     /locus_tag="PLY93_11210"
     CDS             complement(2438..2719)
                     /locus_tag="PLY93_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRP70091.1"
                     /translation="MPTNRQPHTLKENISSVKRQVGKRIRILRKAKNLTQEQVAMGRL
                     SLRAYQRIETGTTNVGVDNLFVIADALDVHISEFFENINLESVDVEENS"
BASE COUNT          759 a          549 c          627 g          865 t
ORIGIN      
        1 agcgcacgtt gtgcatcgtt aatctgcgga attgtccctt tcgccatgcg ccaaaagatt
       61 cacgcgacag acacacagtg acagtacgtt aattacagga gaatagtaca gttttatttt
      121 tctgcgcggt gtaggaggag aattgttaca atgggaaaag ccgccaaaaa aagtaacccc
      181 gcaatttcgc tgcccatacc tgagttgttg gctaaacccg ataagaattc cgtaccggct
      241 gcgccgtctt ctactaaagg agttatcttt tcggaaatag tttgaaaaag acggcgcact
      301 aacggttgtt ggacaagaag aaaaatcact aattctaaaa gaaggtatac caacgcggtg
      361 tatgcccagc gtatcgaatg cagtataccc gatttttttt gttgtagttg tacaagtcgg
      421 atacccgcaa aaagtccata cgcagaaatt accattgtgg caactttttc gagtgcttga
      481 atgtggtaga gagtttttat cggttgaaca aaaatttgaa cttcgacatc ggcattctta
      541 aattcgggta ttattttttt tagtgccggc aatgcatcaa caaatgcact tgcaagaaga
      601 gctgcaccga ttgcatagag tgaataaaaa agatacatcg aagcaaagac gataagcaat
      661 ataccgattg tttgtgttgt cgatttttta tttgtgttgg tgggagtagg gtattctgtc
      721 ggcatgtttt acctcaaaaa gaattgtatg ctttctttac gcttgtgact gaagctgtca
      781 agttcttgac cttttgatat cctttactta tattgctcgc aatgttataa taaccgaaac
      841 caaaagagag agtgtcagca gaaaaagata gaaataaacc gtcgcttttc ggtttatagt
      901 taaaatattt tttctaaagt agttaaagcg aatataatta ataatcaagg aagatattca
      961 tgcaacaaca gcttcgttat attgaacgtt ttgtcgatgg tatacgcgta attgagattc
     1021 ccggcgcgcc cgatgcgccc acattttttt gtatgcacgg ctatggtgcg aatgcgttcg
     1081 acttggccgg gctttcgcag catttagcgt ttccttcaag cacgcgctat ctttttcccg
     1141 atggtatttt aggcgttgac gcagggtttg gcaacgggcg cgcgtggttt cccatcgatt
     1201 gggttgaaat cgagcgcatc cggcgcgaag gtggcttgcg tgatctttct aaaaatttac
     1261 cactgggcat ggccgaggcg cgagagaagg tgattgggct tctgacaaaa ttgtctgtcg
     1321 atattgataa gacggtgatt ggtggatttt ctcagggcgc aatgatggct accgagatat
     1381 tgttatcgca tcggctttat tttaaagggt tgattctttt ttcaggtact cttgtcaatc
     1441 gagtggggtg gcagcaactt ttgcaaaaga agtttatgga gcagaataaa aaactacagt
     1501 ttatccaatc acacgggcgt ttcgatccag tgcttccgtt tcaggcggcg gaatggctca
     1561 accagctttt aaacgatgat ggtggtagag gcgaactggt ggcgtttgat gggcagcatg
     1621 aaataccggc gtcggtggtt gaggcaacga atcgttggtt gcaaagttct ttgtagttta
     1681 tttatttcag tatactatga agacttttct gtcgcgatat tcgctgtgtg ggtgtcgcaa
     1741 tctctattat ttcgtcaaag cgtcgaagtc atctctgcgc ttcggtctaa aataggcatg
     1801 tgtggtagaa aaagcggtgt gctgcccgcc tgaacgacag tttttcttca aaatttaccc
     1861 ttacttttga ccattccagt acccgtggcg tatagttacc cccaacgaca aatttggctt
     1921 ttttttctga cgctttttcc cagaattgga tgagataata aatgagatac gacacacttg
     1981 ttcgtatacc cgcagccatt gtgtgcagcg aatcgtattc cttcgccgta aaacttactt
     2041 tcacaatccc gcaatgtcca tttcctttgt tgtactgccg cgtcacacca cgccggaact
     2101 tcctcttgcg ctcaacaatc cgcaaaaatc gtgtatacat ccgcataatc gcccaacggt
     2161 tggcgagcca agtagggatg gattttcttg gtaaactgat agatgtcttt aacataattg
     2221 tttctctata tattaaatac caaaaacagg tcaaattttc tcacttttta gaaaaaaagt
     2281 ataaatttca gaaaaaatca taattttttt tgagaaaata gtaaatttgg atagttatcc
     2341 ctcgatatga cataatccaa gctcgcagca ggcggtgata gttatcccgt ttttggtgtt
     2401 ggatgaggaa ttttcgggcc tcataaaccg aacgaagcta actattctct tcgacatcca
     2461 ccgattcaag attaatattc tcaaaaaatt cactgatatg cacgtcgagc gcgtcggcga
     2521 tgacaaaaag gttgtcaacg ccgacattgg ttgtaccagt ttcgatacgt tggtacgctc
     2581 ttaaactgag tctacccata gccacttgtt cttgtgttaa attttttgct ttgcgtaata
     2641 tccgaatgcg tttgccgact tgtcgcttta ccgaagaaat attctcttta agagtgtgtg
     2701 gttgtcgatt ggttggcaca atactatttt tttccttatg tggtgttttg agatttagtc
     2761 aatgcgatat aacatgtcaa atgaagaaaa tgtctaaccc
//
LOCUS       DAOMPD010000354         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14594_length_2787_cov_2.384334, whole genome shotgun sequence.
ACCESSION   DAOMPD010000354 DAOMPD010000000
VERSION     DAOMPD010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14594_length_2787_cov_2.384334"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PLY93_11215"
     CDS             complement(<1..1286)
                     /locus_tag="PLY93_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70092.1"
                     /translation="MAEALVEEKKIKDDEFDIHGDLAVSEAEDKSFVILYDSAQKTAS
                     PKTNEHPIDESQAPALDDFSINDSLSNTQQPHPEDAYIDDILSSDDIDTPLDASTSTV
                     YESAPALEDDEPITLSSEELDGILQTDSDDAEDFHPDFPILEENKEHPGESTSVVNKA
                     STTNTENNIDSFVNNIALDEEELPIALSEQDLDEILLEEEGSTVDGADDKSTPATDEL
                     PQGTILVEEPSDGFDEEPIALSDDELDNILADAEASHSEDSSPQSHAAPELLPHEDDT
                     LLTLDKSDDITSIDDANTFDTPEKATVDASESRGFFDSDDDDEPITLSDDELNGILST
                     TEEEESPNTHTEEIALDDAPLAEPLAVEDDDEPIALSNEELDGILMDTEGTPELEQEK
                     TSQAADQGFFEDTGENESITLSADELDGIVASATTE"
     gene            1440..1934
                     /locus_tag="PLY93_11220"
     CDS             1440..1934
                     /locus_tag="PLY93_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70093.1"
                     /translation="MPVKFITSLLFASTIVLHAEEKSDELREKKKIATIDAHAQRKAM
                     LPKLIDAGILMLEGSAYHQFIRTFVDEEEMQRFKASYTENDKVEYMRWGREKGAAILR
                     LLRTLRSTQPDWVEDRACFYTAELGTKSFSFVYQHNAWFIENHSECPKKALPSVPKEH
                     TTTP"
     gene            complement(1940..2299)
                     /locus_tag="PLY93_11225"
     CDS             complement(1940..2299)
                     /locus_tag="PLY93_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70094.1"
                     /translation="MRNVIQNSLIVTAALFFPFCASAEWREGFKNDSTLQLVGEGKLS
                     ESEPQIRANAMAKEAAVMDALSHWIKFCEVESSADGIARFRIENQKYRKVECSSTMCR
                     ARVIIEKEGLRSKCNAK"
     gene            complement(2308..>2787)
                     /locus_tag="PLY93_11230"
     CDS             complement(2308..>2787)
                     /locus_tag="PLY93_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRP70095.1"
                     /translation="FLCRQTDLITAASKTGKWTNIKKGQFVAPEDALQIADKYRKSGS
                     NKVTLCERGYTFGYNNLVVDMRAFEIMRREGLHVVFDATHSTQLPGGGKTSGGDRRMA
                     FPLVRAATAVGLDGVFSEVHPNPPEAKSDATNQLYLNDYEAFLSSVLKIDRLVKSGV"
BASE COUNT          629 a          638 c          725 g          795 t
ORIGIN      
        1 tcctcggtgg ttgcgctggc gacgataccg tcgagctcgt cggcagaaag ggtgatgctt
       61 tcattttcgc ctgtgtcttc gaagaagcct tgatccgccg cttgagatgt tttctcctgt
      121 tccagctccg gcgttccttc ggtatccatg aggatgccat cgagttcttc gtttgaaaga
      181 gcaataggtt cgtcatcgtc ttccacggca agcggctcag ctaacggcgc gtcgtcgagc
      241 gctatttcct cggtgtgagt attggggctc tcttcctctt cggttgtgct taaaattcca
      301 ttgagttcgt cgtccgaaag ggtaatcggt tcgtcgtcat cgtcgctatc gaaaaaacca
      361 cgagactctg aagcgtcaac agttgctttt tctggagtgt caaaagtatt ggcgtcgtca
      421 atcgaggtga tgtcgtcgga cttgtcaaga gtgagtaacg tgtcgtcctc gtggggtaga
      481 agttcgggag cggcatgtga ttggggggag gagtcttcgc tatggcttgc ctcggcgtcg
      541 gcgaggatgt tatctaactc gtcgtctgaa agagctatcg gctcttcgtc aaaaccgtcg
      601 ctcggttcct caaccaaaat tgtgccttga ggtaactcgt cagtggcagg cgtcgattta
      661 tcatcggctc catcgacagt tgagccttcc tcttccaata aaatttcatc taagtcttgc
      721 tcggataatg caatcggtag ttcttcttcg tcgagcgcga tgttgttcac aaacgagtcg
      781 atgttgtttt cggtgtttgt agtcgaggct ttattaacta ccgaagtact ttctcctgga
      841 tgttccttat tttcttcgag aatagggaag tcgggatgaa agtcttcagc gtcgtcagaa
      901 tcggtttgta agataccgtc gagttcttcg ctagatagcg tgatgggttc gtcgtcttca
      961 agcgctgggg cagattcgta cactgtcgag gttgaggcgt cgaggggagt atctatatcg
     1021 tcagaagaca agatgtcgtc gatgtacgca tcttcggggt gtggttgttg tgtatttgaa
     1081 agcgagtcgt taatggaaaa atcgtctaac gctggggctt ggctttcgtc gatgggatgt
     1141 tcgtttgttt ttggggatgc cgtcttttgt gcagagtcgt agagtataac aaacgatttg
     1201 tcttccgctt cgctcacggc taaatcacca tggatatcaa attcatcgtc tttaattttt
     1261 ttttcttcca ctaaagcttc agccattttt atacaccctc tttattatcg gtttttaagg
     1321 ctgcaagctt aaataatcgc ttgtgtgtta agtataaaaa ttttatagag gcttggagct
     1381 tgtgcgcctg cgtaagtttc tcgcggttga cgcaacgcct aaaaagttca acgtggggta
     1441 tgccagtgaa atttataacc agtttgctct ttgcaagtac aatagtgctc cacgccgaag
     1501 aaaaaagcga tgagttacgc gaaaaaaaga aaattgcaac aatcgatgcg catgcacaac
     1561 gaaaagcgat gctgcccaag ctcatcgatg cggggattct catgttggaa ggcagtgcat
     1621 accaccaatt tattcggacg tttgtcgacg aagaagaaat gcaacgtttt aaagcgagtt
     1681 ataccgagaa cgataaagtg gagtatatgc gttggggaag agagaagggg gcagctattt
     1741 tacgcttatt acggacgctc cgcagtacac aacccgattg ggtagaagac agagcatgct
     1801 tttacacggc tgaactgggt acaaaaagtt tttcttttgt ttatcagcac aacgcttggt
     1861 ttattgaaaa ccatagcgaa tgcccaaaga aggcgcttcc cagcgttcct aaggagcaca
     1921 caacgactcc ctaattaatt tatttagcgt tgcatttgct gcgcagcccc tctttttcaa
     1981 tgatgacgcg tgcgcggcac atcgtggaag aacattccac tttgcggtat ttttgatttt
     2041 caatgcggaa gcgagcgatg ccgtcggcgc tcgactctac ctcacaaaat tttatccaat
     2101 ggctgagtgc gtccatcacc gccgcttctt ttgccatggc attcgcgcgg atttgaggtt
     2161 cgctttcgct gagtttcccc tcacccacga gttgtagcgt gctgtcgttc ttaaagccct
     2221 cgcgccattc tgcgctggca caaaagggaa agaacaacgc agcggtcacg ataagactgt
     2281 tttggatgac atttctcatt ctatctatta gacgccactt ttgactaaac ggtcgatttt
     2341 taataccgac gatagaaacg cctcgtaatc gttaaggtac agttggttcg tcgcatccga
     2401 tttcgcctct ggtgggttgg gatgcacctc gctgaagacg ccgtctaacc caacggcggt
     2461 ggcggcgcgc accaatggaa atgccatacg gcggtcgccg cccgaagttt tacccccacc
     2521 aggtagttgg gtggagtgcg tggcatcgaa aacaacgtgg agtccctccc ggcgcataat
     2581 ctcaaacgcg cgcatgtcga cgacgaggtt gttgtaacca aaagtatagc cgcgttcgca
     2641 gagcgtcact ttgtttgacc ccgacttacg gtatttatcg gcgatttgga gcgcgtcctc
     2701 cggtgcgacg aattggcctt ttttgatgtt cgtccacttt ccggttttag acgccgctgt
     2761 aataagatcc gtttggcggc agaggaa
//
LOCUS       DAOMPD010000355         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14659_length_2777_cov_2.663115, whole genome shotgun sequence.
ACCESSION   DAOMPD010000355 DAOMPD010000000
VERSION     DAOMPD010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14659_length_2777_cov_2.663115"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLY93_11235"
     CDS             complement(<1..510)
                     /locus_tag="PLY93_11235"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRP70096.1"
                     /translation="MTTYHFSFPTSITFGNGARHLVADHFKKHGFTTVLIVTDSGLAS
                     LPFFTEYVKELGKNGLTIPIFSGIQGNPTKSQVGAGAAAFKKSGAKAMLAIGGGAALD
                     VAKAIALMAYHPGDIFDYEDEKSGALPVDKEIPYWVAIPTTAGTGSEVGRSAVVADDT
                     THVKKIIFSQ"
     gene            complement(769..2049)
                     /locus_tag="PLY93_11240"
     CDS             complement(769..2049)
                     /locus_tag="PLY93_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HRP70097.1"
                     /translation="MQLLNPTAMELTATICFAIAILHTFLASKIASLAHRFPEGSFAE
                     NIIHFISETEVVFGLWAAALFTTIIATTGSFTGAIDYIQSLNFTEPKFVLVVMVMAAA
                     KPLVSLAEQLINGLTRLIPLNQKIAFYIVALGIGPVLGSVITEPAAMTLLALILKKRL
                     FNQDVSKKFAYATLGLLFVNVSVGGVLTHFAAPPVLMVSAKWNWGLGFMFTHFGWRGF
                     LTTVTGSILMGFIFRREIQKLSLTSDASSRVPAWLTAAHLFLLALTVFFAHYENIFFG
                     VFMFFLGLTVATREYQEPLKLREGLLVGFFLAGLVTLGSLQAYWLKPLLLSLDGSQLY
                     FGATALTAITDNAALTYLGTLVPNLSDSLKYALVAGAVSGGGLTVIANAPNPAGVGIL
                     RSSKIFKDTGVSPLGLFLGALIPTTLAVVFFWII"
     gene            2204..>2777
                     /locus_tag="PLY93_11245"
     CDS             2204..>2777
                     /locus_tag="PLY93_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70098.1"
                     /translation="MKAILNFSNNRISRSNSLLTGLIPLVLVFILACSKAKTPPTQAP
                     IDPNALVLSGPPASVSFPLAYMVATGALKDAAPSVVFKMWMTPDQMRALALDGKTAFM
                     AVPSNVAANLYNKGAQIKPLNISTWGILFMLSRDKERTKLADYKNEEIVLPFRGDMPD
                     IVFQLIAEKEGLDVKKDFKIRYVSSPLDAMQ"
BASE COUNT          812 a          667 c          601 g          697 t
ORIGIN      
        1 ttgcgaaaag ataatttttt tcacatgcgt ggtgtcatcg gcgacgaccg cgctacgtcc
       61 cacctctgag cctgtgccag cggtggtggg gattgccacc caatatggaa tttccttgtc
      121 aacagggagt gctcccgatt tttcgtcttc gtaatcgaaa atatcccctg gatggtatgc
      181 cataagcgcg atagctttcg ccacgtccaa cgccgcgcca ccaccaatcg ccaacattgc
      241 ctttgcccca cttttcttga acgccgcagc accggcaccc acttggcttt tggtgggatt
      301 tccttgtatg ccgctaaaaa ttggtatggt aagaccattt tttcccaatt ctttgacgta
      361 ctcagtaaag aaaggaagcg acgcaaggcc gctatcggtg acaataagca ctgttgtaaa
      421 tccatgcttt ttaaaatggt cggcgacaag atggcgcgcg ccgtttccga acgtgattga
      481 ggtgggaaag ctaaagtggt atgtagtcat gcgctcaagt ttttttcttc catgaggaag
      541 caacgataat tggataaata aaaaattgtg gttataccaa aagtcatgga cagcgttgaa
      601 caggcgggga ggcaaagcga aaactgctac gggattcaca aacctcgtag tattcttggg
      661 atagttatcc ctaaaagaga cataatacaa actttgtctg actgtctctt aatatttcta
      721 tatcttgatt taaagcgctt gttcttacaa atgccttcat tgttttattc atattatcca
      781 aaaaaatacg acagctaaag tagtcggaat caatgctcct aaaaaaagcc ctaaaggcga
      841 aacccctgtg tctttgaata ttttactaga gcgcagtata cctacccccg caggattggg
      901 ggcatttgca atcacggtta accccccacc cgagaccgcg ccagcgacaa gtgcatactt
      961 cagactatcg gacaaatttg gcactaaagt ccccagatac gtcaacgccg cgttgtcggt
     1021 gatagcggtc aatgccgttg cgccaaagta taattgagag ccatcgagcg ataaaagaag
     1081 tggttttaac cagtacgcct gcaaactgcc gagggtaact agccccgcaa gaaaaaaacc
     1141 gactaaaaga ccctcgcgca atttgagtgg ttcttggtat tcgcgcgtcg caacggtgag
     1201 tcctaaaaaa aacataaaaa caccaaagaa aatattttca tagtgggcaa aaaataccgt
     1261 caacgcgaga aggaaaaggt gtgctgcggt tagccaagcc ggtactcgtg aagacgcatc
     1321 gctcgttaat gaaagttttt gtatttcacg acggaaaata aatcccatca gaattgagcc
     1381 cgttaccgtc gttaaaaagc cacgccaacc aaagtgcgta aacataaaac ccaagcccca
     1441 gttccatttt gccgacacca taagtaccgg tggcgcggca aagtgggtca agaccccgcc
     1501 caccgacacg ttgacgaaca acagacccaa ggtcgcatac gcaaattttt tagacacatc
     1561 ttgattaaaa agtcttttct ttaaaataag cgctaaaagc gtcattgccg caggttcggt
     1621 aatcaccgaa ccaagcacgg gaccaatccc caacgccaca atataaaatg caattttttg
     1681 attaagtggg atcaaccgcg tcaagccgtt gatgagttgt tctgcaagag aaactagcgg
     1741 ttttgccgcc gccatcacca tcacgaccaa aacaaacttg ggctctgtaa aattaaggct
     1801 ttggatataa tcaatcgcac cggtaaaact acccgtcgtg gcaatgatgg tggtaaaaag
     1861 agccgctgcc cacaaaccaa atacaacttc cgtttccgaa ataaaatgga tgatattctc
     1921 tgcaaaggaa ccttcgggaa agcgatgtgc aagacttgca attttagacg caagaaatgt
     1981 atggagtatg gcaatggcaa aacaaatcgt cgctgtaagt tccatcgcgg ttgggttaag
     2041 tagctgcatt tttacctcat attcttatat ttttacttag tgcacgaatt atacagggaa
     2101 ctcatcgtca aactttacga ttaaaaatta gactaatgag cctatgcgtg acatcttttc
     2161 tctaaacgct tgacgcaaca atcgagttta catttttcac agtatgaaag cgattctcaa
     2221 tttcagtaat aaccgtataa gtaggtcaaa ttctcttcta acggggttga ttccacttgt
     2281 acttgttttt attctcgctt gcagtaaagc taaaacaccg cccacacaag cacccatcga
     2341 ccccaacgct ttagtgctct ccggtccacc ggcgtcggtg tcgttcccat tggcgtacat
     2401 ggtcgcgacc ggtgcgctga aagatgccgc tcccagcgtg gtatttaaaa tgtggatgac
     2461 ccccgatcaa atgcgtgctc tggcgctcga tggtaaaacc gctttcatgg cagtaccgtc
     2521 gaacgtggcg gcaaatctct acaacaaagg cgctcagatt aaaccgctca atatctctac
     2581 atggggaatc ctttttatgc tctcgcgcga caaagaacgc accaaacttg ccgactataa
     2641 aaacgaagag attgtgttgc ctttccgtgg cgatatgccc gacattgtgt ttcaactcat
     2701 cgccgagaaa gaagggctcg acgtaaaaaa agatttcaaa attcgttatg tcagctcgcc
     2761 tttagatgcg atgcaat
//
LOCUS       DAOMPD010000356         2762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14755_length_2762_cov_2.599926, whole genome shotgun sequence.
ACCESSION   DAOMPD010000356 DAOMPD010000000
VERSION     DAOMPD010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14755_length_2762_cov_2.599926"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLY93_11250"
     CDS             complement(<1..138)
                     /locus_tag="PLY93_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRP70099.1"
                     /translation="MGLVQEITDQNFIDKTASGVVMVDFWAPWCGPCRMVAPVLEDLQ
                     KE"
     gene            707..1099
                     /locus_tag="PLY93_11255"
                     /pseudo
     CDS             707..1099
                     /locus_tag="PLY93_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
     gene            1100..1960
                     /locus_tag="PLY93_11260"
     CDS             1100..1960
                     /locus_tag="PLY93_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRP70100.1"
                     /translation="MDHKPRIFIWGMGLIGASLGLKLKSKGYCVEGAVRSEKSRAVLK
                     QMGFEKIYTSNEESLNALYNADILVLGLTLSDSLSILDLVFSSVIPQKRLIVFDTCST
                     KRSICNFVKEKYPDAPFVGVHPMAGKETSGPGAADAELYANATVYIVPRAQSAIHEKV
                     EALWRDCGAVCTLIDAAEHDRLMAYMSHGLHLNACLLARMANDVASIAPTVYPGAGSF
                     RDMTRIASSSGALWQEIVLSNKDNVVHWLKKLSTEADVLADKLEQNTLDIPALFHEAN
                     EARERIMRTL"
     gene            complement(1944..2297)
                     /locus_tag="PLY93_11265"
     CDS             complement(1944..2297)
                     /locus_tag="PLY93_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70101.1"
                     /translation="MKPLDLQVSIQNSYEAARSEAVRLEKPILIGQQANLDAKHEQAL
                     RDESVSALEAQYLQENLFLEDEYKSPDYENTGERKKEKHPQKKDTAKETVESTEEEIS
                     EDKNKNPQGNFTTYA"
     gene            complement(2306..>2762)
                     /locus_tag="PLY93_11270"
     CDS             complement(2306..>2762)
                     /locus_tag="PLY93_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRP70102.1"
                     /translation="AMQQMKQIAQIEKQLKEKLEQTEINLKTLQQQKSAAPEAFGDAK
                     EEQLVKTLAFKEKLEERLREAFAEASNISEFMRDSEANGAVHVEKTVFPGVTIEINGA
                     SFTVQDEYNNVSFVEEDGRINIIAYVPPDDDDKHFQKNRRKGRYADTQK"
BASE COUNT          662 a          708 c          606 g          786 t
ORIGIN      
        1 ttctttttgt aaatcttcta aaaccggtgc caccatgcgg catggaccac accacggtgc
       61 ccaaaaatcc accatcacga cgcctgacgc tgttttatct ataaaattct gatctgtaat
      121 ttcttgtacg agccccataa taccttcctt ttgacttgac gctttatttc tatgaaagta
      181 tacaaaacaa aaatcgtttt agcaagtttt attattcatg ccttcaatag ccatccacaa
      241 aaagaaccgt cgttcgcgca tgcgcgcttt taccctcact attatcccca actctggcga
      301 gcatgtacgg caatggcgga tttcatttgc atcgatttat tttattctgg ctctttgctt
      361 catccttgtt ggagtgctta ttttctctat tttacgccaa aaacccgtga atactgaaaa
      421 aatggaatcg tccgagcttg cctccgcttg gattgctcgc tgggaactcc ttgaaaccgg
      481 aaaaaatgaa atccgcaaga acttggataa actcaaagaa ataggcgatg aatacttcaa
      541 aacaattttc aaaacgaccc ccatgctcga tgacttttca acgctgggct cgtccgccga
      601 acagaatgca aaactattac ctctcatgag tgtgatgcgc tttttaaacg cgcgcgaaga
      661 ggcctacatt aacatgccca tcggtattgc cgtccattca aaccaaatca cctcgcttta
      721 tggtggccgc gccgaccctt tcggccttga aactagtttc cacaccggca tcgactttgc
      781 cgctggcacg ggcgccccca tttacgtaac cgccgacggc gttgtggtca caaccgacga
      841 tgccggggaa tcgggcttgg ggaaatatgt aaaagttctg cataagtatg gtatcatgac
      901 cgtctatggc cacatgagtc tcatcaccac aacgaaggat aagaaagtaa agcgcggcga
      961 taaaattggc gaagtgggct ccactggccg ctcgacagga ccgcacttgc actacgaagt
     1021 gcatgtgaaa actaccggcc ccgaaaattg gttcgacctc acgtataacc cgatgccgtt
     1081 cattaaagag caactgtaaa tggatcacaa accacgcatt tttatttggg ggatgggact
     1141 tatcggcgcc tcgctgggat taaaactgaa atcaaaaggc tactgcgtcg agggcgccgt
     1201 ccgttcggag aagagtcgtg ctgtcttaaa acaaatgggc tttgaaaaaa tttacacctc
     1261 gaacgaagaa agcctcaacg cactctataa cgcggatata ctcgttttgg ggctgacatt
     1321 gtccgattcg ctctcaatac tcgaccttgt tttttcaagc gttataccac aaaagcgatt
     1381 aattgttttc gacacttgct ccaccaaacg cagcatttgc aattttgtaa aagaaaaata
     1441 tcccgacgcc ccattcgtcg gcgtgcatcc catggctggt aaagagacaa gcggacctgg
     1501 cgctgccgac gcggagctct atgcaaacgc tacagtttac attgtgccgc gcgcacaatc
     1561 tgccatccat gaaaaagttg aagcgctgtg gcgcgattgc ggcgcggtgt gtacactcat
     1621 cgacgctgcc gaacacgacc gcctcatggc gtacatgagc cacggtctgc atctgaacgc
     1681 atgcctttta gcgcggatgg ccaacgacgt tgcttcgatt gcgccgacgg tttacccggg
     1741 cgctggcagc tttcgcgaca tgacgcgtat cgcttcaagc tcgggcgctc tttggcagga
     1801 aatcgttttg agcaataaag ataacgtcgt ccactggctc aaaaaattat cgaccgaagc
     1861 ggacgttctt gccgacaagc ttgaacaaaa taccctcgac atacccgcgc tcttccacga
     1921 agccaacgag gcgcgcgagc gcatcatgcg tacgttgtaa aattcccttg tgggtttttg
     1981 tttttatctt cgcttatttc ttcctcggtt gattcaacag tttcctttgc tgtatctttt
     2041 ttctgagggt gtttttcttt ttttctctcg cctgtatttt cataatcggg ggatttatat
     2101 tcgtcttcta aaaaaagatt ttcttgcaga tattgcgcct cgagtgcgct cacgctttca
     2161 tcacgcagtg cttgctcgtg ttttgcgtct aaattcgctt gttggccaat gagaattggt
     2221 ttttctaagc gcaccgcctc tgaacgcgcc gcttcgtaag aattctgaat actgacttgt
     2281 aaatcaagcg gcttcatcca tccccctatt tctgtgtgtc ggcatagcgc cctttgcggc
     2341 gatttttttg aaaatgttta tcgtcatcgt cgggcggtac gtaagcgatg atattgatgc
     2401 gtccgtcttc ttcgacaaaa ctgacgttgt tatactcgtc ttgcacggta aacgacgcgc
     2461 cgttgatttc aatcgtcacg ccggggaaaa ccgtcttttc gacgtgcaca gcgccgttcg
     2521 cttcgctgtc gcgcataaac tcagagatat tcgacgcttc ggcgaacgct tcgcgcaaac
     2581 gttcctcgag tttttctttg aacgccaacg ttttcactaa ttgttcttct tttgcatcgc
     2641 caaacgcttc gggtgcagcg gacttttgtt gttgcaatgt tttcaaattg atctcagttt
     2701 gttctaattt ttctttgagc tgtttttcaa tttgtgcaat ttgcttcatc tgttgcattg
     2761 ct
//
LOCUS       DAOMPD010000357         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14782_length_2759_cov_3.548077, whole genome shotgun sequence.
ACCESSION   DAOMPD010000357 DAOMPD010000000
VERSION     DAOMPD010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14782_length_2759_cov_3.548077"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2367)
                     /locus_tag="PLY93_11275"
     CDS             complement(<1..2367)
                     /locus_tag="PLY93_11275"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRP70103.1"
                     /translation="MTNEREIENGFIRKLQDLKYQYRDDIRDLISLERNFRQKFEELN
                     KVNLTDSEFARLKDGIVNADVFAAAKLLRERNTFEREDGTPLHYTLVNIKDWCKNSFE
                     VINQLRINTDNSHHRYDVILLINGVPVVQVELKSLEISPRRAMQQIVDYKNDPGNGYT
                     NTLLCFIQIFIVSNRANTWYFANNQNQHFSFDADERFLPVYQYADENNRKITHLDTFA
                     ERFLIKCLLGDMISRYMVLVASEQRLMVMRPYQIYAVKAIVDCIHQNRGNGYIWHTTG
                     SGKTLTSFKASTLLKDNPDIEKCLFVVDRKDLDRQTRQEFNKFQEGCVEENTNTETLV
                     RRMLSTDYADKVIVTTIQKLGLALDGTNKKRYKEQLTPLQSKRIVLIFDECHRSQFGE
                     NHKAIKEFFPNAQLFGFTGTPIFDKNASYKKFEGETASMMTTDDIFQKQLHAYTITHA
                     IEDRNVLRFHVDYFKGEGDAKAHPSDIMAQHAVAETILEKHDAATAGRKFNAIFATAS
                     INDAIEYHRLFKDLQFYYEKERSGYKPLNVACVFSPPADGNKDVQQIQEDLPQERLDN
                     EDAPDEKKAALKSIIDDYNARYGTNHDVNNFDLYYQDIQKRIKDQQYPNHDLPHEHKI
                     DVVIVVDMLLTGFDSKYLNTLYVDKNLKYHSLIQAFSRTNRILNDTKPYGNVLDFRQQ
                     EEAVEEAIALFSGEKIDSPRSIWLVDPGPVVIGKLDNAVAALSKFMESQGLKTAPEEV
                     ANLKGDEARGQFVNLFKEVQRLKTQLDQYTDLTPEDRASIEKIVPPDEL"
BASE COUNT          635 a          641 c          666 g          817 t
ORIGIN      
        1 caactcgtct ggcggcacga tcttctcgat gctcgcacgg tcttcgggtg tgaggtcggt
       61 gtattgatcg agttgtgttt tgaggcgttg tacttctttg aacaaattta caaactgtcc
      121 acgagcttca tcgcctttga ggttagccac ctcttcgggc gcggtcttga gcccttgcga
      181 ttccatgaat ttgctgagtg cggctacagc attatcgagc ttacctataa cgaccgggcc
      241 agggtcgacg agccaaatac tgcgggggct atcgatcttt tcacctgaaa aaagtgcaat
      301 cgcttcttcg acggcctctt cttgctggcg aaaatcgagc acattgccgt aaggtttggt
      361 gtcattcaga attctgttag ttcgcgaaaa agcctgaata aggctatgat acttcaggtt
      421 tttatccaca tagagcgtat tcaggtattt agagtcaaag cccgtcagaa gcatgtcgac
      481 gacaatcacc acatcaatct tatgctcgtg cggcaggtcg tgattcgggt attgctggtc
      541 tttgatgcgt ttctggatat cctgataata gagatcaaaa ttgttcacat catggtttgt
      601 gccataacga gcattgtaat cgtcaataat gctcttgagt gcagccttct tctcgtcggg
      661 ggcatcttcg ttatcgagcc tttcttgcgg caaatcttcc tgaatctgtt gcacatcttt
      721 gttgccatcg gcaggcggag agaatacaca tgcgacattt aggggcttgt accccgatcg
      781 ttccttttca tagtaaaatt gcaaatcttt gaaaagacgg tggtattcta tggcatcgtt
      841 gatggatgct gtggcgaaaa tggcgttaaa ttttctgcca gcagtcgcag catcgtgctt
      901 ttcaagtatg gtctcagcaa ccgcatgttg cgccataatg tcgctggggt gtgcttttgc
      961 atcaccctca cctttgaaat aatccacatg aaaccgcaaa acattgcgat cttcgatcgc
     1021 atgcgtgatg gtatacgcat gcagttgctt ttgaaaaata tcgtcggtcg tcatcatcga
     1081 cgccgtctcc ccctcaaact tcttgtagct ggcgttttta tcaaagatag gcgtaccggt
     1141 aaagccgaac agctgcgcat tggggaaaaa ttctttaatc gccttgtggt tctcgccaaa
     1201 ctgtgagcgg tggcattcgt caaaaatcag tacaatacgc ttgctttgta gtggcgttaa
     1261 ttgttcttta tacctttttt tgttggtccc gtcaagagcg aggcctagct tctgaattgt
     1321 ggttacgata actttgtcgg catagtctgt ggagagcata cgtcgcacaa gtgtttcagt
     1381 gttggtgttc tcttcgacgc agccttcctg aaacttattg aattcctgcc gagtttgccg
     1441 gtcgaggtct ttgcggtcta cgacgaatag gcatttttca atatcgggat tgtctttgag
     1501 caaagtggaa gctttgaacg aagtcagagt tttgccgcta cccgtggtat gccagatata
     1561 gccgtttccg cggttctggt gaatgcaatc aacgatcgcc tttaccgcgt aaatctggta
     1621 ggggcgcatt accatcaatc tttgctcgct ggcgactaat accatatagc ggctaatcat
     1681 gtcgcccaat aggcatttta tgagaaacct ttcggcgaag gtatcgagat gggtgatttt
     1741 cctattgttc tcgtcggcat attggtagac cggtaaaaag cgctcatctg catcaaaact
     1801 gaaatgctgg ttctggttat tggcaaagta ccaggtattg gcccgattgc tcacgataaa
     1861 tatctgaata aagcaaagca gtgtgtttgt ataaccgtta cctggatcat tcttatagtc
     1921 cacgatctgc tgcattgcac gacgggggct gatttcaagc gatttgagtt cgacctgcac
     1981 aaccggtacg ccgttgataa gcagaatcac gtcgtagcga tgatggctat tgtcggtatt
     2041 gatgcgcagc tggttgatta cttcgaaact atttttgcac caatctttga tattcactaa
     2101 cgtatagtgt agcggcgtgc catcctcgcg ttcgaaggtg ttgcgttcgc gtaaaagctt
     2161 ggcggcggca aagacatcgg cattgacgat accgtctttg aggcgcgcaa attccgaatc
     2221 cgtaagatta accttattga gttcctcaaa cttctggcga aagtttctct ctaatgagat
     2281 taaatcccga atatcgtcgc ggtactggta ctttaggtct tgtagcttcc tgatgaagcc
     2341 gttttctatc tctctttcgt ttgtcattct ctggaaaata cttgtttcgg ctgagcattg
     2401 gtctgcaatc gttttctcgg cgtaaagctc cttctttagt tacgttagtt caaaatatcc
     2461 gaaatacagc ctgattcata aagaatcggc atcgggtggc gattaaagat cattatccca
     2521 aacagagaaa cataccttgc tgcaaaattg ctgttctaag cattcatcac ttgtcgcccg
     2581 gccctcctag ccgggctcac aggcgcatct acgtcacaca acaagattta caagctgcgc
     2641 gcacgcgcac ttcgttggcg cgtgtgctcg gcctcgggca cctggactgc ctcctcgcgc
     2701 ggcacagaag tgcattttgc gctcgtcggc aggtgccctc ggcacataaa gtgacgcaa
//
LOCUS       DAOMPD010000358         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14896_length_2741_cov_2.653388, whole genome shotgun sequence.
ACCESSION   DAOMPD010000358 DAOMPD010000000
VERSION     DAOMPD010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14896_length_2741_cov_2.653388"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLY93_11280"
     CDS             <1..475
                     /locus_tag="PLY93_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP70104.1"
                     /translation="MLGGLYLKRKAYDRAIEYYEKARGSMDPNTRYNTLVSQADAMLS
                     SEKPAQLIFPFLKQAIQLRPNADEARFLLARALMREGTMASQDRALEELTVITSGHGS
                     ISLASKSFTLMGIIYYKQGQLNRSLEMFNRALELDPSNSEAFQNRRSVAERLENG"
     gene            complement(472..2334)
                     /locus_tag="PLY93_11285"
     CDS             complement(472..2334)
                     /locus_tag="PLY93_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRP70105.1"
                     /translation="MILVIATEISKEQIEIIVSDHRRLESRTHRTLAPCAAVLKLVQK
                     WEKLYQHENIVNPDGDYLYRKIQLLGRDLSRLLFNAEPPWGDNPDRIHFIADAALQRL
                     PIEILPFQERLLAEKLPILRSLKTDSAPTPKRTPLKSNLFLLVVDVNRNETMRKGLQA
                     EYKNALNENRKYEIEAYQADKIRLDDVRQKLQESAYFHFAGHGEAASLPLWQTDKLTH
                     ESLHGLDLTILHTAFVNSCYSSKTDTRVPTLAEAFINAGAKNYIGYSLPISNDAAYLM
                     AAEFWRHLKASRRPDLAVHKARQAAVEKFGTRDFGWLALHCYASQNSAPRSPVWGAAK
                     IAAAVLLLCIPLAYMLTKNFSTPTRLKESTTRTNTLDKPSPQHSPPPTEKTKKPTTTK
                     NAQREQYKPAAPDSPSLEKLSAGAKRIDPYSFIKPKTLSKMRTFISAAKNGTYVVKVT
                     ECEAPVKNFVYFYDLLPRYVAFETNPNQPAETQYHLGLAAQNLRDATKLTEDAWKKIS
                     LMGCVYGLTEPNNAATVKPAHPKEAASAEKLTIEPQRAKKMQAYYTAIQKPAWQPPSL
                     ECETQSKIFMVWYKGRPFQKPDVPYKSTLSPALYIANLNKLWDTIESEGCIESH"
     gene            complement(2371..2622)
                     /locus_tag="PLY93_11290"
     CDS             complement(2371..2622)
                     /locus_tag="PLY93_11290"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRP70106.1"
                     /translation="MPGTSTLAFEITNIERYGFWILIDEREYFVSFDEYPIFKEATLQ
                     QILDVERPAPNQFHWPSLDADIELEALQNPKKFTQSFKH"
BASE COUNT          637 a          615 c          705 g          784 t
ORIGIN      
        1 aatgctgggc ggtttatact tgaaacgtaa ggcttacgat agagcgattg agtattatga
       61 gaaagcacgc ggctcgatgg atcccaacac acgttacaac accctcgtgt cgcaagcaga
      121 cgcgatgctc tcttccgaaa aacccgcaca actcattttt ccttttttga aacaagcaat
      181 ccagttacgc cccaacgcgg acgaagcgcg ttttctttta gcgcgtgcgc tcatgcgcga
      241 gggtacgatg gcgtctcaag acagggcgct tgaagaactt actgtaatca cctcggggca
      301 tggctcaatc tcgcttgcat cgaaatcgtt tacactgatg ggcattattt attacaaaca
      361 agggcagctc aaccggtcgc ttgaaatgtt caaccgtgct ttagagctcg acccgtcgaa
      421 cagcgaggcg ttccagaatc gtcgttcggt ggccgagcgt ttggaaaatg gttagtggga
      481 ctctatgcaa ccttcgcttt cgatggtgtc ccaaagtttg ttgaggttcg caatataaag
      541 agcgggcgac agcgtgcttt tgtacggaac atcgggtttt tgaaagggtc tgcctttgta
      601 ccaaaccata aaaatttttg actgagtctc gcattcgagc gatggcggtt gccatgcggg
      661 tttttgaatc gcggtataat acgcctgcat tttttttgcc cgttgcggct ctatggtcaa
      721 tttttctgca gaggctgcct ctttggggtg tgccggtttt accgttgccg cattattggg
      781 ttcggtaagg ccgtagacgc aacccatcag cgatattttt ttccatgcgt cttcggttag
      841 ctttgtggcg tctcgtaggt tttgtgccgc caagccaaga tgatactggg tttctgccgg
      901 ttgattgggg ttcgtctcga atgcaacgta gcggggtagt aaatcataaa aatacacaaa
      961 gtttttcacc ggtgcttcgc attcggtaac ttttacaaca tacgtaccat tttttgcggc
     1021 actgatgaac gtccgcattt tggataatgt tttgggttta ataaaactat aaggatcgat
     1081 acgctttgca cccgcagata atttttctaa cgatgggcta tccggcgcag caggtttgta
     1141 ttgttcacgc tgcgcatttt ttgtggttgt gggtttcttt gttttctctg tggggggcgg
     1201 actatgttgg ggggatggct tgtctaaggt attggtgcga gtcgtgctct ctttcaggcg
     1261 agtcggcgtt gaaaagtttt tagtgagcat atacgccaaa gggatacaca acaatagcac
     1321 cgccgctgca atttttgcgg caccccatac gggagagcgc ggcgcagaat tttgagacgc
     1381 gtaacagtgc agcgcaagcc agccaaaatc gcgggtgcca aatttttcta ccgccgcttg
     1441 ccgcgctttg tggacggcca agtcggggcg gcgcgaagct ttcaaatgac gccaaaactc
     1501 tgccgccatt aaatatgccg cgtcgtttga aatgggcagc gaatacccga tataattttt
     1561 agcgcctgcg ttgataaacg cttcggccaa ggtcggcacg cgcgtatcgg tttttgaaga
     1621 gtagcaacta ttgacgaaag cggtatgcag gatggtgagg tcaagcccgt gcaaagattc
     1681 gtgcgtcagc ttatcggttt gccaaagggg cagcgatgcc gcttcgccgt ggcctgcaaa
     1741 atgaaagtac gccgattctt gtagtttctg ccgcacgtca tcgagacgaa ttttatccgc
     1801 ctgataggct tcaatttcat actttcgatt ttcattgagc gcgtttttat attcggcctg
     1861 taaacctttg cgcatcgttt cgttacggtt cacatcgacg accagcaaaa atagattgct
     1921 cttcagcggc gttctttttg gggtgggtgc cgaatcggtt ttaaggctgc gcaaaatggg
     1981 cagcttttcg gcaagaaggc gctcttgaaa aggcagaatt tcgatgggca accgttgcag
     2041 cgcggcgtct gcaataaaat gaatgcggtc agggttatcg ccccacggcg gctcggcgtt
     2101 aaaaagcaat cgtgacaaat ctctgccgag gagttgaatt ttccgatata gataatcacc
     2161 gtcggggttg acgatgtttt cgtgttggta tagtttttcc catttttgaa cgagcttcaa
     2221 caccgccgcg cacggggcaa gcgtgcggtg cgtacgcgat tcaaggcggc ggtggtctga
     2281 aacgatgatt tctatttgtt ctttagatat ttcggtagca ataaccagaa tcacgcacgc
     2341 cgccttgggc ttatgttttg ggatgtcaaa tcagtgttta aaactttggg tgaacttctt
     2401 aggattttgt aaagcctcaa gctcaatgtc tgcgtcaaga cttggccaat gaaattgatt
     2461 tggggctggc ctttcgacat caagaatttg ctgcaaagtt gcttctttga aaataggata
     2521 ttcatcaaat gaaacaaaat attctcgctc gtcgattaat atccaaaagc catatctttc
     2581 gatattggtt atttcaaacg ctaaagtgct tgttccaggc attgataaat cattatattt
     2641 aatgtttatg gcgtcaaacc tttcttggct agtcgtgagc agctacgagc ctgtgcgcca
     2701 actcgcacac caagcccttc ttcccttcta aaaatcaaaa t
//
LOCUS       DAOMPD010000359         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14898_length_2741_cov_2.325391, whole genome shotgun sequence.
ACCESSION   DAOMPD010000359 DAOMPD010000000
VERSION     DAOMPD010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14898_length_2741_cov_2.325391"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..425
                     /locus_tag="PLY93_11295"
     CDS             135..425
                     /locus_tag="PLY93_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70107.1"
                     /translation="MKTATINNHPIAPMISTHQQRGAWSYQRKPSTYPAKTIKGSFSD
                     RAQSSPKALTFREILEREMGGWGSKKTVVGAAAKAETSAEAETTQFLTGYWA"
     gene            complement(398..943)
                     /locus_tag="PLY93_11300"
     CDS             complement(398..943)
                     /locus_tag="PLY93_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70108.1"
                     /translation="MNLFRKLTSALTDYLTLYGEVGSAITQRGGAFLRSRWQRFLELP
                     IEEKGHAVLTMLVVLFTFLPWRGYAIQFGDSEPRKHGIYSDDFAIILVGCLISLLPHL
                     WRMLPNEPHFLKNGALYRYSGISIVALFALLNWFFPTRIAQAAEASFTWSFFVFEAVV
                     AVWVVTGVLGAKCYAQYPVKN"
     gene            complement(1014..2195)
                     /locus_tag="PLY93_11305"
     CDS             complement(1014..2195)
                     /locus_tag="PLY93_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP70109.1"
                     /translation="MSKYEGLMDFTITDEMHEMLDMAHELCRNEVVPRRAEMDEHEKF
                     PHEIFQKFGQAGLFGIMFEEKYGGLGLSSFLTVLAIEVMSEYCLGVATSAGAIKLGAL
                     PIEHGGTEEQKQKYLTPLAKGEKIAAFALTEPGAGSDVPNLKTFAEKKGDRYILNGTK
                     QWITNAGVADIYTVFALTDKTKGPRGISCFIVEKGTKGLSFGKLEQKMGIRCSHTRQV
                     IMEDMEVPAENLVGLKPNAGFIHAIKTLISSRPFIATMGTGLATGAYKEALKYTRQRE
                     QFGKNIINFQAVQHMLAEMVTKIEASRLLAYRAAKYSLNNHPEAARYSAMAKLMSSET
                     AVQVCNDALQLHGGYGFTKDYPVEKMYRDAKILTIYEGTSQIQKNEIGAYAIKDAGQL
                     K"
     gene            2311..2613
                     /locus_tag="PLY93_11310"
     CDS             2311..2613
                     /locus_tag="PLY93_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRP70110.1"
                     /translation="MGFSDFKDMMSQMNQMRQQAKEVQKKLGAMRVTAETGAGLVKAT
                     VDGEANLIDLVIDTSLLDADEIKVLPKLIKKAIQEAQKDARKQVMSDMKGMMGGMM"
BASE COUNT          705 a          673 c          687 g          676 t
ORIGIN      
        1 gtccgaggct tcaaaaacta tggtttggcc aaagattgtc cgacctcccc gccgaaggta
       61 agtaatttta gttgccagat acacccactt atagtattat gtcatatagc tgggtggttg
      121 tctctgcgga gactatgaaa acagcaacaa taaacaacca cccaatcgca cccatgattt
      181 ctacgcacca gcagagaggc gcgtggagct accaacggaa gccgagcacg taccctgcaa
      241 aaacaataaa gggttcattc tcggataggg cgcaatcgtc accgaaagct ctcaccttcc
      301 gtgaaattct cgaaagagaa atgggtggtt ggggctcaaa aaaaacagtg gtgggagcgg
      361 cagcgaaagc tgagacttct gccgaagccg agaccactca gttcttaaca gggtattggg
      421 cgtagcattt tgcgcccaat acccctgtaa ctacccacac cgcaacaact gcttcaaaaa
      481 caaaaaagct ccatgtaaag gaagcttctg ctgcttgtgc aatgcgcgta gggaaaaacc
      541 aatttaagag tgcaaaaagc gcaacgatgg aaatcccact ataccggtag agggctccgt
      601 ttttaagaaa gtggggttca ttcggcagca tccgccacaa gtgcggcaaa agcgatataa
      661 ggcagccgac taaaataatc gcaaaatcgt cgctatagat accgtgtttg cgcggctcac
      721 tatctccaaa ttggatggca taaccacgcc acggtagaaa agtaaacaag acgacaagca
      781 tcgtcaaaac ggcatggccc ttctcttcga tgggcaactc cagaaaccgc tgccagcggc
      841 tgcgcaaaaa tgcaccgccg cgttgagtga tagcgctacc gacctcaccg tagagcgtga
      901 ggtaatcggt aagcgcagaa gtgagttttc taaaaaggtt catggtagcg ctggtcgagt
      961 agcctcggct cgatggcttt ggctactcac tgactggcgt atcgagacca acgttatttt
     1021 aattggccag cgtctttgat ggcgtacgcg ccaatttcat ttttctgaat ttggctggtg
     1081 ccttcgtaga tggtcaaaat ttttgcgtcg cggtacattt tttcaaccgg atagtccttg
     1141 gtaaaaccat acccgccgtg gagctggagt gcgtcgttgc acacctggac cgccgtttca
     1201 gacgacatga gttttgccat cgccgaataa cgcgccgctt cggggtggtt gttcaaagag
     1261 tacttcgccg cgcggtacgc taagaggcgc gacgcttcga ttttagtcac catctcggcg
     1321 agcatgtgct gcaccgcttg gaagttgatg atgttcttgc cgaactgctc gcgctggcgt
     1381 gtatacttga gcgcttcttt atatgcgccg gtggcaagac ctgttcccat ggtggcgata
     1441 aacgggcgtg aagagatgag agttttaatc gcgtggataa accctgcatt cggtttcaat
     1501 cccactaagt tttcggcggg tacctccata tcttccataa tgacttgccg tgtgtgcgag
     1561 cagcggatgc ccattttttg ttcgagttta ccgaaagaca atcctttagt acccttttca
     1621 acgataaaac aagagatacc ccgtggcccc ttggttttgt cggtgagcgc aaacacagtg
     1681 tagatgtcgg cgacgccggc gttcgtaatc cactgtttgg tgccattcaa gatgtaccga
     1741 tcgccttttt tctcggcaaa ggttttaagg ttggggacat cggaacctgc ccctggttcg
     1801 gtgagtgcga acgcggcgat tttttcgcct ttagcgagcg gtgtgagata tttctgtttt
     1861 tgctcttcgg tgccaccgtg ttcaatcggc agcgcaccga gtttaatggc gcctgcgctg
     1921 gtggcaacgc ccaagcaata ctcgctcatc acttcaatcg cgagcacggt caagaaagat
     1981 gaaagtccca aaccaccata cttctcttca aacatgatgc cgaaaagacc ggcttggcca
     2041 aatttttgaa aaatttcgtg cgggaatttt tcgtgttcgt ccatttcggc gcggcgcggc
     2101 accacttcgt tgcggcaaag ctcatgcgcc atatcaagca tttcatgcat ttcatcggtg
     2161 atggtaaaat ccatcaagcc ttcgtatttt gacataattt cctctctaaa agtaagatta
     2221 atttcttctg tcgctgttcc atcgttagac agtcggacaa gtcgattata tttacgattt
     2281 acgcagcgta tcgatattca atcgtactct atgggatttt cagacttcaa agatatgatg
     2341 agccagatga accaaatgcg ccaacaggcc aaagaggttc aaaaaaaatt gggagccatg
     2401 cgcgtcaccg ccgaaaccgg tgcgggactt gtgaaagcca ccgttgatgg cgaggcgaac
     2461 ctcatcgatt tagtcatcga caccagcctt ttggacgccg atgaaattaa agtattgcct
     2521 aaactcatta aaaaggcaat ccaagaagcg caaaaagatg cgcgtaagca agtaatgtcc
     2581 gacatgaaag ggatgatggg cggtatgatg taatgtcgat tccgttttca ttacactgat
     2641 gccgcccgaa cttttggcgg cgattgaagc gattgcagcg ctacccggga ttggccgccg
     2701 ctccgccatg cgcatcgcgt ttgattttgt caaaaaaggg c
//
LOCUS       DAOMPD010000360         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_14990_length_2727_cov_2.123877, whole genome shotgun sequence.
ACCESSION   DAOMPD010000360 DAOMPD010000000
VERSION     DAOMPD010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14990_length_2727_cov_2.123877"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLY93_11315"
     CDS             <1..317
                     /locus_tag="PLY93_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70111.1"
                     /translation="RKRLGDRFTGTTSLSYSYFTDDMASLTLGVNYTYLNEGDATING
                     EPEKTSGFSRHLFGASLAWATPERNIIVTVGYNPALHVPDIQYNFPATHVISLGVNYV
                     LR"
     gene            304..861
                     /locus_tag="PLY93_11320"
     CDS             304..861
                     /locus_tag="PLY93_11320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HRP70112.1"
                     /translation="MYYVKKTNQLRWMITHFFLFFFLGITIHCSNLIPDGSDLRTHNP
                     NSIVNVGDSAPNFSLVSTAHTTLNLTTALSGKNAVVLYFTMWCSVCTSHTDEILQTLV
                     NAYPNTRFVFVDYVSASLNDTISMQHYSGYDGKPIDVAFDQAQRVMNTYGATMASTIV
                     IDSAGKVRMNETYKKVRLDNLLGTL"
     gene            858..1109
                     /locus_tag="PLY93_11325"
     CDS             858..1109
                     /locus_tag="PLY93_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRP70113.1"
                     /translation="MKAALACLFLVFYCLPIWAESGETLFREKCVQCHALPDKEALNG
                     KQWKAVLKTMQKRMQEAKKPMLTDEELQKVYCFLTDGNC"
     gene            complement(1142..>2727)
                     /locus_tag="PLY93_11330"
     CDS             complement(1142..>2727)
                     /locus_tag="PLY93_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRP70114.1"
                     /translation="ASTLQAEVERRRAFAIIAHPDAGKTTLTEKLLLYGGAIHLAGHV
                     RARKDRKSTKSDFMTLEQERGISVSTAALQFEYDGMVMNLLDTPGHQDFSEDTYRTLM
                     AVDSAIMLIDASRGVEPQTIKLFKICRERNLPITTFINKMDMPALEAFELLDNVEKVL
                     GIAAVPLVWPMGSGRDFKGYFDLYEKKLHRFERTVGGAHRAPEAVGGIESDEVRNLMG
                     DAAHDKFCEDVQIASDALPPFDKEKYLQGTMTPVFFGSAVTNFGVEHLLSHLKELAPA
                     PRPILLDNSDIIEPTDNRFTGFVFKMQANMNKLHRDRVAFVRITSGKFERGMSVSVPR
                     LDKDVKLANPVAFFGQERTTIDTAYAGDIIGIISPGIYRLGDVLYERVSGADKAPEFH
                     PLPRFAPDVYARIVLTDTGKAKQFRKGISELADEGVVQLFNVNDASPLIGVIGQLQFE
                     VFKYRIEDEYGAPIRLEPLPFECSRWISSSDASKAGRFDKIVNDEKGNPVLLFESQFR
                     LRSFQEQNPDVELHLHPIH"
BASE COUNT          692 a          709 c          653 g          673 t
ORIGIN      
        1 accgcaagcg cttgggcgat cgcttcacgg gcacgacatc cttaagctat tcgtacttca
       61 ccgacgatat ggctagccta acccttgggg taaactacac gtatctcaat gagggcgacg
      121 ccaccatcaa cggcgaacct gaaaagacat cgggattttc gcggcatctg ttcggcgcat
      181 cgcttgcgtg ggcaacaccc gagcgcaaca tcattgtgac cgtaggttac aacccagcgc
      241 tccatgttcc cgatattcag tacaactttc ccgcaaccca cgttatttct ttaggagtta
      301 attatgtatt acgttaaaaa aacaaaccaa ttaagatgga tgattaccca tttctttctt
      361 ttcttttttc tggggataac tatccactgc tccaatctaa tcccagacgg cagcgattta
      421 cgtacccaca atccgaattc gattgtgaac gtgggagata gtgcgcctaa tttttcactt
      481 gtctccaccg cgcacactac gctcaatctc accaccgctc tttcgggaaa aaacgccgtg
      541 gtgctctact tcaccatgtg gtgctcggtg tgcactagcc acaccgacga aatcctacaa
      601 acattggtga atgcataccc taacacgcgc tttgtgttcg tcgattatgt ttccgcatcg
      661 ctcaacgaca ccatctcgat gcagcactac agcggctatg acggcaaacc catcgatgtc
      721 gcttttgacc aagcacagcg cgtcatgaac acatacggcg caacaatggc gagcaccatt
      781 gtgatcgata gcgcgggcaa agtacgcatg aacgaaacgt ataaaaaagt acgcctcgat
      841 aatttattgg gtaccctatg aaagctgcac tcgcttgctt atttcttgtt ttttattgct
      901 tgcccatttg ggcagaaagc ggtgaaactc tttttcgaga aaaatgtgtc cagtgccatg
      961 cactgcccga caaagaagcc ctgaatggca aacaatggaa ggccgttttg aaaacgatgc
     1021 aaaagcggat gcaagaagct aaaaaaccga tgctgactga cgaggagttg caaaaagtgt
     1081 attgttttct tacggatggg aattgttaac ctttttaagc cgttgctgcg cttttaaaaa
     1141 attaatggat tgggtgtaaa tgcaactcca cgtcggggtt ttgttcttga aaagaacgta
     1201 agcgaaactg gctttcaaaa agtagcaccg gatttccttt ttcgtcgttc acaattttat
     1261 caaacctgcc cgccttcgat gcatcggagg aggatatcca ccgagagcac tcaaacggca
     1321 acggttctaa acgaatgggc gcaccgtact cgtcttcaat gcggtatttg aaaacctcaa
     1381 actgcaactg gccgatgact ccaatgagcg gcgaagcgtc gttcacgttg aaaagttgca
     1441 caaccccttc gtcggcaagt tcgctaatcc ctttacggaa ttgctttgcc ttgccagtgt
     1501 cggtaagcac gatgcgcgcg tacacgtcgg gggcaaagcg cggcagcgga tggaactcgg
     1561 gcgctttgtc cgcacccgat acacgctcat aaagcacatc acccaagcgg taaatacccg
     1621 ggctaataat accgataata tcgccggcat acgcggtatc gatggtggtg cgctcttggc
     1681 caaagaacgc aacgggattg gcgagcttca cgtccttatc aagacggggc acgctcacag
     1741 acatgccgcg ctcaaacttg ccgctcgtga tgcgtacgaa cgccacacgg tcgcggtgga
     1801 gcttgttcat attcgcctgc atcttaaaca caaagccggt gaaacggttg tccgtaggtt
     1861 cgatgatgtc gctattatca agcaaaatag ggcgtggtgc gggagcaagc tctttaagat
     1921 gcgagagcaa atgttcgacg ccaaagttgg tgactgcgct accaaaaaag actggcgtca
     1981 tcgtcccttg taagtatttc tctttatcga atggagggag agcgtccgag gcaatctgca
     2041 cgtcttcgca aaatttatcg tgcgcggcat cgcccatgag attacgcact tcgtcggatt
     2101 caatgccgcc caccgcttcg ggtgccctgt gcgcaccgcc cacagtcctt tcaaaacggt
     2161 ggagcttctt ttcgtataaa tcgaaatacc ccttgaaatc gcgccccgac cccatcggcc
     2221 atacgagcgg gaccgcagca attcctaaca ctttctcgac gttatctaaa agctcaaacg
     2281 cttcaagcgc aggcatatcc attttgttaa tgaacgtggt gatcggtaaa tttcgttcgc
     2341 gacaaatttt gaaaagtttg atagtctgcg gttcgacacc acgcgaagca tcgatgagca
     2401 taatcgctga gtcaaccgcc atgagagtgc ggtacgtgtc ctcactaaag tcttggtggc
     2461 caggggtgtc gagcagattc atcaccatgc cgtcgtattc aaattggagt gcggcagtgg
     2521 acaccgagat accgcgctct tgttccaagg tcatgaaatc cgacttggtc gattttcgat
     2581 ctttacgcgc gcgtacatga cctgcgagat ggattgctcc gccatagaga agcaattttt
     2641 ctgttagcgt tgtttttcct gcgtcggggt gtgcaataat cgcaaacgca cggcggcgtt
     2701 caacttcagc ttgtagagtg ctcgctc
//
LOCUS       DAOMPD010000361         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15062_length_2715_cov_3.272556, whole genome shotgun sequence.
ACCESSION   DAOMPD010000361 DAOMPD010000000
VERSION     DAOMPD010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15062_length_2715_cov_3.272556"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLY93_11335"
     CDS             complement(<1..391)
                     /locus_tag="PLY93_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70115.1"
                     /translation="MEIPLSLIFLGVLVFVSYTAQAMTGFGSIVIALTIGALFFEMRT
                     ILPVLVALNLLLCIWILYRGFRAIDTSVLFQKILPLMGAGVALGVLVSQTWDGPLLRR
                     LFGGIIAAFALRELVFLLFFQNKTQKPL"
     gene            637..1647
                     /locus_tag="PLY93_11340"
     CDS             637..1647
                     /locus_tag="PLY93_11340"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRP70116.1"
                     /translation="MKKKIITIFVALFTFLLIVNFRWIIYLGHLAKHQAKVILFKERI
                     VDRLKKNNLDPKERNALTNTLKLRAALEQLYEIKNSKSYTAYYALGRDALGYNITVAS
                     ALSLKPESFHFWPIGSFAYLGFFDKEYAETWAKKYHDKGYDVHLAEIGGYSTLGWFED
                     PLYSSQLKWGTFNLAHLLAHEIAHEKLYIKNNTELSEMIASFIEKKAAPDAMRVAQLW
                     HGEDKKASLFKKRRNELVTKLLSLREKLQQLYASSLNDIQKLNRKKEWITILKKEIEQ
                     KRDYYFVKGSIEINNASLAQIKRYTPNSPMFEKIFDACLEKDKAHVYSCWFSAVEKQN
                     KK"
     gene            complement(1723..>2715)
                     /gene="fusA"
                     /locus_tag="PLY93_11345"
     CDS             complement(1723..>2715)
                     /gene="fusA"
                     /locus_tag="PLY93_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804029.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRP70117.1"
                     /translation="IEEVATGEIAAAVGLKDTTTGDTLCMEDSPAILEKMTFPAPVIH
                     IAVEPKTKGDQEKMGIALGRLAEEDPTFHVRTDEETGQTIIAGMGELHLEVLVDRMKR
                     EFKVEANVGKPQVAYRETIRKAVEQEGKYIKQTGGRGQYGHVWIKVEPLEPGGGYVFE
                     NKIVGGVVPREYVQPVNKGIEEALTSGVMAGYPVVDVKVALFDGSYHDVDSSELAFKI
                     AGSMAFKDACRKASPVLLEPIMKVEVTTPEDYMGDVVGDLNRRRGKVNAMNQRGNVRV
                     VDALVPLAEMFGYATELRSITQGRAAYTMQFEHYEEVPPNISKEISEKAAAKKG"
BASE COUNT          779 a          671 c          573 g          692 t
ORIGIN      
        1 ggagtggctt ttgtgttttg ttttgaaaaa acaataaaaa cacaagctca cgcagtgcga
       61 acgccgcaat gatgccccca aagaggcggc gtaaaagagg accatcccat gtttggctca
      121 ctaacacgcc taaagccacc cccgctccca tgagcggtag gattttttgg aacaatacgc
      181 tcgtgtcaat cgcgcggaac ccacggtaca aaatccaaat acacaacaga agattgagcg
      241 caaccaagac cggcagtatc gtgcgcattt caaaaaagag tgcgccgatg gtgagcgcaa
      301 tgacgatgct accgaaaccg gtcatcgcct gcgcggtata cgaaacgaaa acaagaacac
      361 ctaaaaaaat taaacttaat ggtatttcca tatttggata acgatgctta ttatttgagc
      421 ttatgcgtca atccgtgaaa aaataaatgc catttcctta agagcgtgtg cgacaactca
      481 tgcagtgtac aaaaatttgc aaaaaaattg cctcagcgag ttggcgcaca agctcttaaa
      541 tgacttgcaa gagcaaagaa gtattgaagc ggtgtagcta tgccttgtaa gtttatattc
      601 cacatactcg tttttttgcc cctgcgctaa acatatatga aaaaaaaaat tattactatt
      661 tttgtcgcgc tctttacttt tctactcatc gtcaattttc gctggattat ctatttaggg
      721 cacctcgcca aacaccaagc caaagtcatt cttttcaaag agcggattgt agatcgtctt
      781 aaaaaaaaca atctcgaccc caaagagaga aatgcgctaa caaacacgct caagctgcgt
      841 gcagcgctcg aacaacttta tgaaatcaaa aattcaaaaa gctatacggc atactatgcc
      901 ttgggacgcg acgcattggg gtataacatc accgttgcgt cagcgttgag cctcaagccg
      961 gagtcgttcc atttttggcc cattggcagc tttgcgtact taggcttttt tgacaaagaa
     1021 tacgcagaga catgggctaa aaaataccac gataaaggct acgatgtcca cctggctgaa
     1081 attggcggtt actcgacact cggttggttt gaagacccgc tctattcgtc gcaactcaaa
     1141 tggggaactt ttaacttggc gcatcttttg gcacacgaga ttgcgcacga aaaactttac
     1201 atcaaaaaca acactgagct ttcagagatg atcgcatcgt ttattgaaaa aaaggcagcc
     1261 cccgatgcca tgcgagtagc gcagctttgg cacggggagg ataaaaaggc ttctcttttc
     1321 aaaaaacgac gtaacgagct cgtcacaaaa ttgttatcac tcagagagaa actgcaacaa
     1381 ctctacgctt cttccttgaa cgacattcaa aaattaaaca ggaaaaagga atggataact
     1441 attctaaaaa aggaaatcga gcaaaaaaga gattattact ttgtcaaagg ttctatcgaa
     1501 atcaataatg cttctttggc acagatcaag cgatacacgc caaattcgcc tatgtttgag
     1561 aaaatttttg acgcatgcct agaaaaagat aaagcgcacg tgtattcgtg ttggtttagc
     1621 gcagtagaaa aacaaaataa aaagtaacat gcccattggg taatgccgcg ctgatacacg
     1681 acaactgtcg tctaccagac ttcaacccca aataggcgag atctaccctt tcttcgctgc
     1741 tgccttctct gaaatttctt tcgagatatt cggcggtact tcttcgtaat gctcgaactg
     1801 catggtgtac gccgcacggc cttgagtaat gctgcgtaac tcggttgcat aaccaaacat
     1861 ttcagccaat ggcacaagag catccaccac gcgcacatta ccacgctggt tcattgcgtt
     1921 cactttacca cggcggcggt ttaagtcgcc gacgacatca cccatatagt cttcgggagt
     1981 tgtcacttcg accttcataa ttggttcgag cagtacagga ctcgctttgc ggcaagcgtc
     2041 tttgaacgcc atagaaccgg caatcttaaa cgccaattca gatgaatcca catcgtggta
     2101 tgaaccgtcg aaaagagcga ctttaacgtc gacgaccgga taacccgcca tcacgcccga
     2161 tgtgagcgct tcctcaatcc ctttgttaac cggctgcacg tattcgcgtg gaactacacc
     2221 gcccacaatt ttgttttcaa acacataacc accgcctggt tcgagcggtt cgactttaat
     2281 ccaaacgtgt ccgtattgac cgcggccacc ggtttgttta atatacttac cttcttgttc
     2341 gaccgcttta cggatggttt cacggtacgc cacttgcggt ttgccgacgt tcgcttcgac
     2401 cttgaactcg cgtttcatac ggtcgacgag aacttcgaga tggagttcac ccatccccgc
     2461 aataatcgtt tgtccagttt cttcgtcagt gcggacgtgg aatgtggggt cttcttctgc
     2521 aagacgcccc aacgcaatcc ccattttttc ttggtcaccc tttgtcttcg gctcgaccgc
     2581 gatatgaatc acaggtgcgg ggaatgtcat tttttcaaga attgccggcg aatcttccat
     2641 acaaagagta tcgcccgtgg tggtgtcttt aagacccaca gcagccgcga tttcacctgt
     2701 agccacttcc tcaat
//
LOCUS       DAOMPD010000362         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15064_length_2715_cov_1.992857, whole genome shotgun sequence.
ACCESSION   DAOMPD010000362 DAOMPD010000000
VERSION     DAOMPD010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15064_length_2715_cov_1.992857"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLY93_11350"
     CDS             <1..189
                     /locus_tag="PLY93_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70118.1"
                     /translation="AEANRAGEGKAALEFKFRPSFVENVANPLLLGIENRKEGEFHDL
                     PFACRGNVFSGNCYKQNW"
     gene            266..820
                     /locus_tag="PLY93_11355"
     CDS             266..820
                     /locus_tag="PLY93_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70119.1"
                     /translation="MDYREQIYIENKKLSAMIIFILRKLPGKLNRIALCKNLFYAEMH
                     YFQKAGKSISGATYLHIESSPMPRFFNEILESMVAKEEVCVVPHVAPEVIDGQQIMVL
                     RGMVFECAAPLIPILTREELRAVRMVSSTLQNEMNLETRYFPQYYQHYVQTGLYEGIP
                     FVTFPKRPHLQFKAWGRKIYRLMY"
     gene            complement(843..>2715)
                     /locus_tag="PLY93_11360"
     CDS             complement(843..>2715)
                     /locus_tag="PLY93_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRP70120.1"
                     /translation="IKPAPNMGARGVMFLQKSDHLSFAFEFAAVQDKNHEIILEHFVP
                     AREISVDALSFNGHVFLTGVADRIIEIKDKHFFIETGHTLPGNFTQHEYNLILATLTR
                     YAQALSTLANKPYHGALKGDLRLTPHGALAIGEIAGRLSGGFMSTHTYPAAHEKNLMQ
                     MFIRLMQHDATVVLSFEEAIRTTHVAIERSLYADAGEIIQLKTADELLTLKKKYDKEG
                     VIHIAGNYQNGDLIQNLQSNVGKVYHTVLRAPTLKDAERTFAHIKNELTFSTRIPEYN
                     SRKMSKGARAKWNNAYCWVCKVCDGVYCASSVPGMGGPGGMNSFQDNINALQEYKIAP
                     QYIHPTPNTGEPDITFRLFRNEALRFSAPILSAPITGSVTNMGGSITEYDYAMETGSA
                     MARLNLIPTFGDGASADKYKIGLHAIEALGYGIPVFKPRADQKELISRIYDAEKAGAT
                     AWAIDIDGVSFKTMKLKSAETRHKSLEELKELAAASQLPFIIKGVMSEHDAEVAALAG
                     AAAIVVSNHGGRVLDALPGTARVLPRIAAWVKQNAPQLQVLADGGVRSGSDVFRMLAL
                     GAEAVLIGRPIAIIAVGYGRMGVQSLIQSYTDELKQTLRVLNLPSLAAIRPEHITKY"
BASE COUNT          641 a          630 c          726 g          718 t
ORIGIN      
        1 gccgaggcca atcgagcagg ggagggaaag gcggcgcttg agtttaaatt tcgtcccagt
       61 tttgtggaga acgtggcaaa tccgttactc ctcggcattg agaaccgtaa agaaggcgag
      121 tttcacgatt taccgtttgc ttgccgcggt aatgtgtttt cggggaactg ctacaaacag
      181 aattggtaag atagtctctc atcgcgcgtg tcgcataggc tcttattaga gttgccgcac
      241 ggcctcgctt tccactgtct gcattgtgga ttaccgcgaa cagatttata tcgagaacaa
      301 aaaattaagc gcgatgatta tttttattct gcgtaaattg ccgggaaaac tcaatcgcat
      361 tgcgctttgt aaaaatcttt tttatgctga gatgcattat tttcagaaag caggtaaaag
      421 catcagtggc gcgacttact tgcatattga atcatccccc atgccacgct tttttaacga
      481 aattttggaa agtatggtgg ccaaagaaga agtctgcgtg gtgccgcacg ttgcccctga
      541 agtcatcgac gggcagcaaa ttatggtgct aagaggaatg gtttttgagt gcgccgcgcc
      601 gcttattccg attttaaccc gcgaagagct acgcgcagtg cgtatggttt catcgacgct
      661 gcaaaatgaa atgaacttag agacgcggta ttttccgcag tattatcaac actatgtcca
      721 aacagggctt tatgaaggta tcccctttgt aacattcccc aagagaccac acttgcaatt
      781 taaggcgtgg ggcagaaaaa tttatcgttt gatgtattag taggatgctt ctcaacagcc
      841 tgttagtatt tcgtaatatg ttcaggtcga attgcagcga gggaaggtaa attcaacacc
      901 cgtagagttt gcttgagctc gtcggtgtaa ctctggatga ggctttgcac acccatgcgg
      961 ccgtagccca cggcgatgat cgcaatggga cgaccgatca acaccgcctc tgcccccaat
     1021 gccagcatac gaaaaacatc cgaacctgaa cgcacgccgc cgtcggctaa aacttgtagt
     1081 tgcggtgcat tctgtttcac ccatgcagcg atacgaggta aaacccgcgc tgtacccggg
     1141 agtgcatcga gcacccgccc gccgtggtta gacactacaa tagccgctgc gcccgcgagc
     1201 gcggccacct cggcgtcgtg ttcgctcatc acgcctttaa tgataaaagg aagctgtgaa
     1261 gcggcggcga gttctttgag ctcttcgagc gatttgtgcc gagtctctgc acttttgagt
     1321 ttcatcgtct taaacgaaac gccgtcgatg tcgattgccc acgcggtcgc tcccgctttc
     1381 tcggcatcgt agatgcgact gatgagttct ttctggtcgg cgcggggctt aaagacgggt
     1441 atgccatacc ctaaggcttc aatcgcgtgt aggccaattt tatatttatc cgccgacgca
     1501 ccgtcgccga atgtgggtat aagattgagg cgcgccatcg ccgagccagt ttccattgca
     1561 tagtcgtatt cagtgatgga gccacccatg ttggtgacgc tgccggtgat gggggctgaa
     1621 agtatcggcg cagaaaagcg taatgcctcg ttacgaaaaa gacgaaatgt gatgtcgggc
     1681 tcgccagtat tcggcgtggg gtggatgtat tgcggtgcaa ttttatattc ttgaagcgcg
     1741 ttgatgttgt cttgaaaact attcatgccg cctggaccgc ccatcccagg gacactcgaa
     1801 gcgcaataga cgccgtcgca cactttacac acccagcaat acgcgttatt ccattttgca
     1861 cgcgcgcctt tcgacatctt ccgcgagttg tattcgggga tgcgggttga aaatgtaagt
     1921 tcgtttttga tgtgcgcaaa agtgcgctcg gcgtctttca atgtgggtgc gcgaagaaca
     1981 gtgtggtata ccttgcccac gttagattgc aagttttgga tgagatcgcc attttggtag
     2041 tttccggcga tgtggattac tccctctttg tcgtatttct ttttgagcgt taaaagttca
     2101 tcggctgttt tcagctggat gatttctcct gcgtcggcgt aaagcgagcg ttcgatggcg
     2161 acgtgcgttg tgcggattgc ttcttcaaaa gagaggacaa cggtggcgtc gtgttgcatc
     2221 agacggataa acatctgcat cagatttttt tcatgcgcgg cagggtaggt atgcgtgctc
     2281 ataaaaccac cggaaagtct accggcaatt tcaccgatag caagtgcgcc gtggggggtt
     2341 aagcgcaaat ctcccttgag cgcgccgtgg taaggcttgt tggcgagcgt ggagagcgct
     2401 tgggcgtagc gggtcagtgt tgctaaaatg agattgtatt catgctgtgt aaaatttccg
     2461 ggcagggtat ggccggtttc aataaaaaaa tgtttgtctt taatctcaat aatccggtcg
     2521 gcgacaccgg ttaaaaaaac atggccgtta aacgaaagtg catcgactga aatttcacgc
     2581 gcggggacaa agtgctctaa aataatttcg tgatttttgt cttgcactgc ggcaaattca
     2641 aatgcaaacg agaggtgatc cgatttttga agaaacatca ccccgcgtgc gcccatattt
     2701 ggcgcaggct tgatg
//
LOCUS       DAOMPD010000363         2712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15081_length_2712_cov_2.669176, whole genome shotgun sequence.
ACCESSION   DAOMPD010000363 DAOMPD010000000
VERSION     DAOMPD010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15081_length_2712_cov_2.669176"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..490)
                     /locus_tag="PLY93_11365"
     CDS             complement(44..490)
                     /locus_tag="PLY93_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70121.1"
                     /translation="MVGTSMLYARDVVGTLLKDTVATFSDSYDLIIYSLSAAIQRDLR
                     AQKQSVLEETAATNERIEIYLESKGILSRRDRQPITRGDFARIVFQRFDLPRGFFTKL
                     FETTSWYFRDAVRIGLFSENDTAEGTMSTREMLSVFTRAEALSRSR"
     gene            complement(558..1487)
                     /locus_tag="PLY93_11370"
     CDS             complement(558..1487)
                     /locus_tag="PLY93_11370"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRP70122.1"
                     /translation="MEFIEELNLIPPSLSGSVVVMGDFDGVHTGHYALIHEAVSHAKA
                     ENLTSILLTYEPSPKKIMKKLAYDSRLTTHAEKQQLLANTDLDIVIFYPVTAKTLQIS
                     ARNFLRSFLLERLSMRRLVMGGDHHFGHNRRGNAKYLQAAKKRYGFALTVIDEQLTLG
                     KRTSSSRIRTALAEGKMDITAEILGRPYSVTGKVVHGERRGRSIGFPTANLSIDPEKL
                     LPALGVYAGVSVLENGERICSVANLGQKPTVGAFPVGLEVHCIQFKKDIYGQNLTFEF
                     TKRIREEKKFASLDALKAQIEEDVKEAKSVVVF"
     gene            1738..>2712
                     /locus_tag="PLY93_11375"
     CDS             1738..>2712
                     /locus_tag="PLY93_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRP70123.1"
                     /translation="MRVRSKLIIALSLVIAVAIIPISFYIVNKFEHSSFQKAKEEGEA
                     QSQFFVRSVFNLLLTNGGNIPATRVDAKEVLKIFEGQKAQGLVAMKAIFLSRDKENNG
                     STLASWPHWIEKESVTELPPTYALRACSDHPQNRCMVFSANMGLSDEAPSLLVSLEYA
                     EAVVLAPIQELKQNLLFAVVIALMLVLILAVLLGFQMTKPLKILEKGTRELAKGNLTY
                     TVALERADEFGELARSFNFMVTSLNRHIEELKEKDRELNAAQTIQRAFLPETLPQSAS
                     IGFAAHFTPMAQVGGDLYDVFETGEGYGILIADVSGHGVPAALITGMAK"
BASE COUNT          754 a          638 c          627 g          693 t
ORIGIN      
        1 ctgcgcctaa agagcactat aaaacgattt tggacaagca cgtttagcgc gaacggctca
       61 gcgcttcggc gcgcgtaaat acggacagca tttcgcgcgt cgacattgta ccttcggcgg
      121 tatcgttttc agaaaaaagg ccgatacgca ctgcatcgcg aaaataccac gaggtggttt
      181 caaaaagctt agtaaaaaaa ccacgcggta aatcgaaccg ttgaaaaacg atgcgggcaa
      241 aatcgccgcg cgtgataggt tgtctgtcgc gccgtgagag aatgcccttg gattccaaat
      301 aaatttcaat ccgttcgttt gtagctgccg tttcttccag cacgctttgt ttttgtgcgc
      361 ggaggtcgcg ttgaatggct gcactcaacg aatagatgat tagatcgtac gaatctgaaa
      421 aagtggcaac ggtgtccttg agaagcgtac cgacgacatc gcgtgcgtag agcatagaag
      481 tacccaccaa aagcgtccag agaacaaaaa aaatattttg gaaaaaccgt accatcttct
      541 ttaatatacg gtaaaaatca aaaaacaacg acactttttg cctctttcac gtcttcctca
      601 atttgtgctt ttagcgcatc gagactggca aattttttct cttcacggat acgttttgtg
      661 aattcaaacg ttaaattttg gccgtaaata tctttcttaa attggataca atgcacttct
      721 aaaccaacgg ggaatgcacc cactgtcggc ttttgtccca aattagcaac ggaacaaatg
      781 cgttcaccgt tttcaagaac gctcacaccg gcgtagactc ccaacgcggg caacaatttc
      841 tcagggtcga tcgataggtt tgccgtggga aagcctatcg acctgccacg gcgctcgccg
      901 tgcaccacct tccctgtaac ggaatagggg cggcctaaaa tttcagcggt gatgtccatc
      961 ttgccttcgg ctaaagccgt acgaatgcgc gacgaggacg ttctcttgcc cagcgttaat
     1021 tgttcgtcga taacggtgag ggcaaatcca taccgttttt tggccgcttg aagatacttt
     1081 gcattaccgc gacggttgtg tccgaagtgg tgatcaccgc ccatcactaa gcgccgcatg
     1141 gataaccgtt ccaaaagaaa cgaacgtaaa aagttgcggg cgcttatctg taatgttttt
     1201 gccgtaacgg ggtaaaaaat aacgatatcc aaatcggtgt tcgctaataa ctgctgcttt
     1261 tctgcatgtg tggtgaggcg gctatcgtac gccaactttt tcataatttt cttgggactg
     1321 ggttcgtacg tcaatagaat cgacgttaag ttttcagctt tcgcatggct caccgcttcg
     1381 tgtatgagtg cataatgacc agtatgaacg ccatcaaagt cgcccatgac aacgacactc
     1441 cccgacaagg agggggggat taagtttaac tcttcaataa attccacaga gcatgacaaa
     1501 tataaaggtc tttacacgct gtcctttcaa ataatgaagt catcgatgcc cagtggccgt
     1561 aaacgtaaac gtagaaaaat tgcaaaccat aagcgcaaga aaaagcgtcg tgcaaatcgt
     1621 cataaaaaga aataatttgc tataaaaaat gaagcaaggg aagacgaaaa atcttccttt
     1681 ttgcttcatt ttagtaaata atgatggctg tcctactcat aaagtagagc atgccttgtg
     1741 cgtgtacgca gcaaactcat tattgctctt tctttagtta ttgcagttgc cattatcccc
     1801 atttcattct atatcgtaaa caagtttgag cattccagct ttcaaaaagc gaaagaagag
     1861 ggcgaagctc aatcccaatt ttttgtacgt agcgttttta atttgttgct caccaacggc
     1921 ggcaatattc cagccacgcg cgtcgatgcg aaagaagttc taaaaatttt tgagggacaa
     1981 aaagcacaag gccttgtagc gatgaaagca atttttctct cgcgcgataa agaaaataac
     2041 ggtagtactt tagccagttg gccgcattgg atagaaaaag aaagtgttac tgaacttccc
     2101 cccacctatg cgctgcgcgc ttgcagcgac catccgcaaa accgctgtat ggttttttcg
     2161 gcgaatatgg gattgtccga cgaagcgccc agtttgctcg tctcgttaga atacgcagag
     2221 gcggtggtgc tggcgcccat ccaggagttg aaacaaaatt tactctttgc cgtcgtgata
     2281 gctttaatgc ttgttttaat tctcgcagtg ctgttgggct ttcaaatgac aaagccgctc
     2341 aaaattttag agaagggcac acgcgaatta gcaaaaggca atttgacgta caccgttgcg
     2401 cttgaacgcg ccgatgaatt tggtgagttg gcgcgctctt ttaacttcat ggtcacctcg
     2461 ctcaaccgcc acatcgaaga gctcaaagaa aaagaccgcg aactcaacgc ggcacaaacc
     2521 atccaacgcg catttttacc cgaaacgctg ccgcaaagcg catcgattgg ctttgcggcg
     2581 cactttaccc ccatggcgca ggtgggcggc gacttatacg atgtttttga aacgggggaa
     2641 ggttatggaa ttttaatcgc cgatgtgagc ggccacggcg taccagcggc gctcatcacc
     2701 ggcatggcca aa
//
LOCUS       DAOMPD010000364         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15118_length_2708_cov_2.391255, whole genome shotgun sequence.
ACCESSION   DAOMPD010000364 DAOMPD010000000
VERSION     DAOMPD010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15118_length_2708_cov_2.391255"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLY93_11380"
     CDS             <1..315
                     /locus_tag="PLY93_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70124.1"
                     /translation="AGRIARTSFYPIPAVDAAVLFFNREENLPRPHLRALLKAAFWGK
                     RKTLASALRKNPFWSDESVAVSWNEKLTAIQQAHERIFAKRADDISVEEFFVVYDALN
                     RS"
     gene            complement(499..978)
                     /gene="bfr"
                     /locus_tag="PLY93_11385"
     CDS             complement(499..978)
                     /gene="bfr"
                     /locus_tag="PLY93_11385"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRP70125.1"
                     /translation="MQGDKKALEFLNAHLKNELTAINQYFLHAKMLKNWGMDKLAAHE
                     QKESIEEMKHADRLIERILFLEGLPNLQDLGRLRIGENVEEILQCDLDMEIGVLPLLK
                     EAIQHCEKVGDFISRIIFADILASEEAHIDHLETQLGLIQKMGLPNYIQLQSSSVAG"
     gene            complement(1321..2616)
                     /locus_tag="PLY93_11390"
     CDS             complement(1321..2616)
                     /locus_tag="PLY93_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRP70126.1"
                     /translation="MRTFLSPVDNRFVPWLASKLYFPLLKAMQNIQKIHIRDEDIQTL
                     KKFKNERLLFFTNHPTTAEPPIVFYLGRLLGQRFKFMASRQVFNWQGGLIGWAIRRIG
                     AYSVIAGVADRDAMKLTRKILSEPTGKLVIFPEGEPTSGEVDTLMPFQPGVAQLGLWG
                     LEDARKADSTADINVWYAFMKFTIDAPSEKIKADLDRNLKKLEKKLGIEALQKDKHLL
                     HRFLTVGRVILEQAEKDFGIADKTEKGFDYRIGRVRHAMLDRLAEKLQAANYDKSEDA
                     IHKWRKLFALVELHQLKYPDPKLPKLDTKTIREAHKDAVLIFDFIVMKRDYIIDNPTP
                     ERLYEWLDRYESYLFKKIPRALGGVPSHLPRHAHILFGTPYKLSEVVTENRRDRRIAV
                     ENFTNRLRSDMQKLLESSASLTKPLFSNEEIQAVRAKIK"
BASE COUNT          659 a          596 c          688 g          765 t
ORIGIN      
        1 gcgggacgta tcgcgcgcac gagtttttat cctataccgg cggttgacgc cgccgtttta
       61 ttttttaatc gtgaagagaa tttaccacgc cctcacttgc gcgcgctact taaagcggcg
      121 ttttggggga agcgaaaaac gttagcgagc gcgctcagaa aaaatccatt ttggagcgat
      181 gaaagcgtgg cagtctcttg gaacgaaaaa cttaccgcta ttcaacaggc gcacgaaaga
      241 attttcgcaa agcgcgccga cgacatttcg gtcgaggagt tttttgtcgt gtacgacgcg
      301 ttaaaccgca gctaaatttg gcgaggttgt cccaaagtca caaaccgcac caaacagacg
      361 cggcggcaaa gcgaagccaa caatgggatt tgttttgcaa accctggagt gttggcgtgc
      421 gatttgcaaa aaaattactt cttggaaatg cacgctgcgc gagttggcgc acaggctccc
      481 aggcggagag ctttctgttt atcctgcgac cgacgagctc tgtagctgga tataattggg
      541 aagtcccatt ttttgaataa gccccaactg cgtttcaagg tggtcgatgt gcgcttcttc
      601 ggacgctaaa atatctgcaa aaatgatgcg tgaaataaaa tctccaactt tttcgcagtg
      661 ttggattgct tctttaagta aaggtaaaac gccgatttcc atatcgaggt cgcattgaag
      721 aatctcttcg acattttcac cgatgcgtaa tcgacctaaa tcttgcaggt tgggcagtcc
      781 ttcgagaaat aagatacgtt cgatgagtct atcggcgtgc ttcatttctt caatggattc
      841 tttttgctcg tgtgcggcaa gtttatccat gccccaattc ttgagcattt ttgcgtggag
      901 aaaatactgg ttaattgccg ttagttcatt cttgaggtgc gcatttaaga actcaagagc
      961 ttttttatca ccttgcatgg tgtataattt attatctata caagacggcc agtaaaaagt
     1021 ggctgagaaa ctatgcttgt gtgcgccaac tcgctggagc acaggttctt gaatttatca
     1081 atcacataac atacttactc aattcgtcac tgtcggttgt ttcctcgaaa atttgatggc
     1141 agtaaatgca ttggtatgcg acgcgaatgg gttttaccga tacccccgtc aacaaagcaa
     1201 atttgcccca aaaagaatac ttgtttttgc ggattgcgta tgggcttttg cgatcggtgc
     1261 cgcacgcaca tttttttaaa tcggtcgttg ttccagtcat aatagcttta tatctatagt
     1321 ttattttatt tttgcacgca cggcttgaat ttcttcgtta ctaaaaagcg gctttgtcaa
     1381 ggatgccgag ctctccaaca gcttttgcat gtcgctgcgc aaacgattgg taaaattttc
     1441 aacggctatg cggcggtcgc ggcggttttc ggtaaccacc tcggacaact tatacggtgt
     1501 gccgaaaaga atgtgcgcgt gccgtggcaa gtgagaggga actcctccca atgcacgtgg
     1561 gatttttttg aagagatacg attcataacg gtcaagccat tcgtagagac gctcgggcgt
     1621 ggggttgtcg atgatgtagt cgcgcttcat cacaatgaag tcgaaaataa gtaccgcgtc
     1681 tttgtgcgcc tcgcggatgg ttttcgtgtc gagctttggt aatttggggt cggggtattt
     1741 gagctggtgt aattcgacga gtgcaaaaag tttgcgccac ttgtggatgg cgtcttcgct
     1801 cttgtcgtag ttcgccgctt ggagtttttc agcgagacgg tcgagcatcg cgtggcgcac
     1861 gcggccaatg cggtaatcga atcctttttc tgttttgtcg gcaatgccaa aatctttttc
     1921 ggcttgttcg aggatgaccc gccccactgt taaaaaacga tgtagtaggt gtttatcttt
     1981 ttgtaacgcc tcgataccta atttcttttc aagttttttt aagtttcgat ctaaatcagc
     2041 cttaattttc tcagaaggcg catcgatcgt gaatttcatg aacgcgtacc atacattgat
     2101 gtcggctgtg gaatctgctt tccgtgcatc ttctaaaccc caaagcccca actgagccac
     2161 acccggctgg aacggcatga gcgtatccac ttcacccgag gtgggctcgc cttcggggaa
     2221 gatgacgagc ttacctgtgg gctcggataa aatttttcgg gtgagtttca tcgcgtcgcg
     2281 gtcggcgaca ccggcaatca ccgagtacgc accgatacgg cggatagccc aaccgatgag
     2341 accgccttgc cagttgaata cctgccgcga tgccatgaac ttgaaacgtt gtcccaagag
     2401 acggcctaaa taaaatacaa tcggcggttc ggcagtggtg gggtggttgg tgaaaaaaag
     2461 caagcgttcg tttttgaatt ttttgagggt ttggatgtct tcgtctcgga tatggatttt
     2521 ttggatgttt tgcatcgcct tgaggagtgg gaaataaagc ttcgacgcca accacggcac
     2581 gaagcggtta tcgacagggg ataaaaaagt acgcataaca gaaagtcaaa gctggctttg
     2641 gagcgtcaat ggtttctctg gcgggctact aaaaaataac gattgggtta ggagcgtatc
     2701 tcaagacc
//
LOCUS       DAOMPD010000365         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15199_length_2698_cov_2.743473, whole genome shotgun sequence.
ACCESSION   DAOMPD010000365 DAOMPD010000000
VERSION     DAOMPD010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15199_length_2698_cov_2.743473"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..2392)
                     /locus_tag="PLY93_11395"
     CDS             complement(32..2392)
                     /locus_tag="PLY93_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRP70127.1"
                     /translation="MAKINKVGFTDRLKNFILDTLSGNFDETKKFQKRLQSAIRFIIT
                     AAKKFLDDEVLLRSASISYAIVVSFVPTLVVIMMVGARFINIEEYFEQINDMARLGGM
                     QVDLEPYFNVIRNLLKNAGAIGGIGLLVLMFSATSVLRNVDDAINRIWRVIRKRPMVQ
                     KIGGFMMVVIFGPIVLTIGISSAQWLVKQFASPNLVQVKIIKDTVQILGDQHMLLVQN
                     EKGKPFREKNILGAVDLLVNNEAIVFSAKHNEIIPAERREVYEHARNVQPKELRDSTF
                     VDYARVNNRELIITGNGILLTSLDNGKTFYTKKFYRQRRDHAVEVGFTRLYVLSDQNI
                     VIIGTEGLILLSKDAGRTWRPNFQESATKHLRQMARLPNGSWLLVGDHAALVTTDNGL
                     TFNDYQPVLAQLQIQKVSLTGIAVSPKAGYIVGEAGLLLVTRDGGLSWKSVPIAQTMF
                     FEAVAVATDGTAVAVGYDGLIRYSQFLPDGSMQWLSAATNTSEVLRAVQFYPKENRFI
                     IVGDNYQMVSQQMSNDKLHAREFTVIQKAPFWRRLISALGNIVIPFMVIFFLFFLIYK
                     IIPYTKVSAKAAASGAVITSIAWVIFLILYKYYVTHFSKGTAALYGTLALVPLTLLML
                     YISSLIMLFGAELAFFVQYPHLLRLSTKKTDDERHKRQLWYGISMLNRLAQSFNTGKV
                     DCSTGALVEHCNGDQEEFMFIIERLKTRGYVTQADGQLWLLAMNPDRIQISTLVEDLD
                     PSDYSIPNFSPKNPFMVSVKKHFDKLEENRGKVFRSITLSQLMNKI"
     gene            complement(2441..>2698)
                     /locus_tag="PLY93_11400"
     CDS             complement(2441..>2698)
                     /locus_tag="PLY93_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70128.1"
                     /translation="KSTHRSCLYLRAFAFAMQDDFDTAYNLIKKIKRGMREIDNRLLY
                     LIVAVGANKEDISALKRFIHRQSYRENASSLRRLAQKILLS"
BASE COUNT          760 a          599 c          580 g          759 t
ORIGIN      
        1 tcaattatcg acatttttta aaattcgtta tctatatttt attcatgagt tgcgataacg
       61 taatcgagcg gaacactttg cctcgattct cttcgagttt atcgaagtgt tttttgaccg
      121 agaccataaa cggatttttt ggcgaaaagt tgggtatcga gtaatccgat gggtcaaggt
      181 cctccacgag cgttgaaatt tgaatgcgat cgggattcat cgctaagagc caaagttggc
      241 cgtcagcctg cgttacatag ccgcgcgtct tgagtcgttc aataataaac atgaactctt
      301 cttgatcgcc gttgcaatgt tcgacaagtg cgcccgtgga gcaatcaact ttaccggtat
      361 tgaagctctg cgctaatcgg ttaagcatac taatgccgta ccagagttgg cgcttatgcc
      421 tttcgtcgtc ggtttttttt gtactcaagc gcaagaggtg cgggtattgc acaaagaacg
      481 caagctctgc cccaaagagc ataatgagcg acgaaatata aagcatcaaa agagtcaacg
      541 gcaccaacgc taaagttcca taaagcgcag cggtgccttt tgaaaaatga gtgacgtaat
      601 atttgtagag tattaaaaaa ataacccacg caatcgatgt aataaccgcg ccggaagccg
      661 ctgctttcgc cgaaactttc gtgtatggga ttattttata gataagaaag aacaagaaaa
      721 atataaccat aaagggaatc acgatatttc ctaaagccga gatgagtctg cgccaaaagg
      781 gcgccttttg aatgacagta aattcacgcg cgtgtagctt atcgttgctc atttgctgag
      841 ataccatttg gtagttatcg cccacaatga taaaacgatt ctccttggga taaaactgta
      901 cggcacgcaa aacctctgaa gtatttgttg ccgcagacag ccattgcatt gagccatcgg
      961 gtaaaaattg agaatagcga atcaagccat cgtaacccac tgcaactgct gttccatccg
     1021 tggcgacagc gacagcttca aaaaacatcg tttgtgctat cggcacgctt ttccaagaga
     1081 gcccaccgtc gcgcgtcacc aaaagaagtc ctgcttcgcc gacaatgtac cctgctttgg
     1141 gtgaaactgc aatgccggtc aacgatactt tctgaatttg cagttgcgcc aaaaccggtt
     1201 ggtagtcgtt aaatgttaaa ccgttgtctg ttgtgacaag agctgcgtga tcgccgacga
     1261 gcagccacga accattcggc aaacgcgcca tttgtcttaa atgttttgta gcgctctctt
     1321 gaaaattagg ccgccaagtt ctgccggcat ctttagaaag gaggatcaac ccttcggtac
     1381 caatgataac gatattttga tcggacaaaa cataaagacg ggtgaatccc acttcgacgg
     1441 cgtggtcgcg tctttggcga taaaactttt ttgtataaaa tgtcttaccg ttatcaaggc
     1501 tcgtcagcaa aataccgttt ccagtaataa taagctcacg gttatttaca cgcgcataat
     1561 cgacaaacgt cgaatcgcgc aattcctttg gctgtacgtt tcgagcgtgt tcgtacactt
     1621 cgcgtctctc ggcaggtata atttcattgt gtttagcgct aaaaacaata gcttcattat
     1681 tgacgagtaa atccacagcg cccaaaatat ttttctcgcg gaatggtttc cctttttcat
     1741 tttgaactaa gagcatgtgt tggtcgccca aaatctgtac tgtatctttg ataattttta
     1801 cttggactaa attaggcgaa gcgaattgtt tcacaagcca ctgcgccgaa ctgatgccaa
     1861 tcgtaaggac aatcggccca aaaataacga ccatcataaa accgccaatt ttttgtacca
     1921 ttggtcgttt acggatcacg cgccatattc gattgattgc gtcgtcaacg ttacgtagca
     1981 cgcttgtcgc tgagaacatg aggaccaaaa gacctatacc gccgattgcg ccggcgtttt
     2041 ttagtaggtt acgaatgacg ttgaaatacg gttcaagatc gacttgcatc ccccccaagc
     2101 gcgccatgtc gtttatctgc tcaaaatact cttcgatgtt aataaatcgt gcccccacca
     2161 tcatgatgac aacgagcgtt ggcacaaaac tcactacgat agcatagcta atcgacgcac
     2221 tacgcaacag aacttcgtcg tctaaaaatt ttttagctgc agtgattata aatcggatgg
     2281 cggattgtaa ccgtttttga aattttttgg tttcgtcgaa attccccgaa agggtatcga
     2341 gtataaaatt tttgagtcta tcagtgaaac caactttgtt aattttagcc atattttata
     2401 cgaaaagcat aaaagagtct atatgtcaac cgagtgtttt ttacgacaac agtatttttt
     2461 gagcaagacg gcgcagtgaa gaggcattct ctctatagga ttggcggtgt ataaatcgct
     2521 ttaacgcgct tatgtcttct ttatttgctc ccaccgcaac gataaggtat aagagccggt
     2581 tatcaatctc gcgcattcct cgttttattt tctttatgag attatacgca gtatcaaaat
     2641 cgtcttgcat tgcaaaagca aacgcgcgta agtagaggca tgagcgatgc gtcgattt
//
LOCUS       DAOMPD010000366         2695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15215_length_2695_cov_2.397348, whole genome shotgun sequence.
ACCESSION   DAOMPD010000366 DAOMPD010000000
VERSION     DAOMPD010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_2695_cov_2.397348"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLY93_11405"
     CDS             complement(<1..700)
                     /locus_tag="PLY93_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRP70129.1"
                     /translation="MIDWSNTLASVVQGLKPSGIRAFFDIVATRKDCISLGVGEPDFV
                     TPQVIVDHAVQALRDGYTHYTGNSGLLSLRREIAKYLDHEYGLVYNPEKEILITVGVS
                     QGVDVAFRALLSENEGVLYGEPCYVSYEPMIRLAGGLPQKIDTSAETQFVLTSGAIRK
                     RIDAEKGNSKILFLNYPSNPTGASIAEAELESIAALAEEKNLIVISDEIYGELSYEKK
                     HIPFSTLPRMKERTL"
     gene            complement(739..1938)
                     /locus_tag="PLY93_11410"
     CDS             complement(739..1938)
                     /locus_tag="PLY93_11410"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRP70130.1"
                     /translation="MLLTPLEQVDCTGKKILLRVDFNVPMDKATGVISDDTRIVKSLP
                     TIELLLSRGASLIIVSHLGRPEKKGDPAFTLAPIAKRLAELIPQTKIHFSTEAIGPIP
                     EKFVSELKPAEILVLENIRYYPEETSKDEKVRFEFAKKLCKLAEIYVNDAFGAAHRAH
                     ASTYECAKVLPSYAGLLLKKEIDILEKLTTQPQRPFVAVIGGSKVSSKLAVLENLVLK
                     ADSILVGGAMAYTFLKSRLIEVGSSLVEKEYLSQAFQIIDKAAYNKHELLLPDDHVVS
                     SEFSEKGKIKTVKRAIPDGFMGMDIGPKTIDRYSNIIKSAKTVLWNGPMGVFEMKKFS
                     VGTFAIAKAMAKTKGTTIVGGGDSLFALAQSGLEDKMTHISTGGGATLEYLEGKKLPG
                     VEALLKK"
     gene            2036..>2695
                     /locus_tag="PLY93_11415"
     CDS             2036..>2695
                     /locus_tag="PLY93_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP70131.1"
                     /translation="MPEKSHARQNVSRKVIFEQALDAEKKGNLNLALEKYKMALQIDQ
                     KFFDAWLNAGAIYTRQGKSNKAVVCYQRALVSKNDVRAHYNLAVEYYKNNNFVGAQKS
                     LKSVLRLDSRYLNAHLLLGYVFGKLGENDKSEISIRNALKIDPQNIQAQTALALLYFH
                     TDRSELALRQVNLLLSRSPNDKVLLGLSAKINLSGGDLRSAAAGFKSIAARDNDLKQF
                     YT"
BASE COUNT          707 a          578 c          648 g          762 t
ORIGIN      
        1 gcagtgtgcg ctctttcatt cggggtagcg ttgagaaagg aatatgtttt ttttcataag
       61 agagttcgcc gtaaatttca tcggagatga caatgagatt tttctcctcg gccaaggcgg
      121 caatgctttc aagctctgct tcggcgatcg acgcaccggt ggggtttgag gggtaattca
      181 aaaataaaat ttttgaatta cctttttctg catcgatgcg ctttcggatt gctccagagg
      241 tgagcacaaa ttgggtttct gcgctcgtgt caattttttg tggcaatcca cccgctaaac
      301 ggatcatcgg ttcgtatgaa acataacacg gttctccata gagcacgcct tcattttctg
      361 agaggagggc gcggaatgca acatcgacgc cttgtgaaac gccgacggtg atgaggattt
      421 ctttttcggg attatagaca agaccgtatt catggtctaa gtattttgca atttcgcggc
      481 ggagcgataa gagtccagaa tttcctgtgt agtgcgtgta gccgtcgcgt agcgcttgaa
      541 ccgcgtggtc gacaataacc tgcggggtca caaaatcggg ttcgcctacg cccaacgaaa
      601 tgcaatcttt gcgcgttgca acaatgtcaa aaaaggcacg aatccctgag ggttttaatc
      661 cttggacaac cgaggctaat gtatttgacc aatcaatcat gcacagctct tcgcttaaag
      721 tctgtggagt ttaccaaatc acttttttag gagcgcttca acacccggca gtttttttcc
      781 ttccaagtat tcgagggttg ccccgccgcc ggttgaaata tgcgtcattt tgtcttctaa
      841 gcccgattga gcaagagcaa agagagagtc gccaccgcca acaatcgtgg tgccttttgt
      901 tttcgccatc gcttttgcaa tcgcaaaggt tcctacagaa aatttcttca tttcaaaaac
      961 acccatcggg ccattccaga gcaccgtttt cgcgcttttt ataatgttcg aataacggtc
     1021 gatagttttg gggccaatgt ccatacccat aaaaccgtcg gggattgctc gcttcaccgt
     1081 tttaattttt cctttttcac taaactcaga agaaaccacg tgatcgtcgg ggagtagtaa
     1141 ttcgtgttta ttatacgccg ctttgtcgat gatttggaac gcttgactca aatactcttt
     1201 ttccacgagc gagctcccca cctctatcaa acgcgatttt aagaacgtgt acgccatcgc
     1261 tccaccaaca agaattgaat ccgctttgag aactaaattt tccagaacag ccaatttgga
     1321 agatactttt gaaccgccaa tcacggcgac gaaagggcgt tgtggttggg tagttagttt
     1381 ttccagaatg tcaatttctt tttttagaag aagtccggcg taggagggta agacttttgc
     1441 gcattcataa gtcgaagcgt gcgcgcggtg tgctgcgcca aaagcatcgt tgacgtagat
     1501 ttcagcgagt ttacaaagtt ttttagcaaa ctcgaagcgt accttttcgt ctttagatgt
     1561 ttcttcagga tagtaacgga tgttttctaa aactaaaatt tctgcaggtt ttagttcact
     1621 cacaaatttt tcaggaatcg gccctatagc ttcggttgaa aaatgtattt tagtttgtgg
     1681 gatcaactct gcgaggcgct tggcgatggg cgctaaagtg aacgcagggt cgcctttttt
     1741 ttcggggcgg cctaagtgag atacaatgat gagcgatgcg ccacgcgaga ggagtagctc
     1801 gattgtgggt agacttttga cgatacgggt gtcatcgcta atgacgccgg tggccttgtc
     1861 catagggacg ttaaagtcga cacgcaacag gattttttta ccggtgcagt cgacctgttc
     1921 cagcggagta agtaacatga acggataagc caaagtggac tacgggcggt aaatcgtttt
     1981 ctaaactaaa aatttacgtc gaaaagttcg ttaattttac cgatttataa agagtatgcc
     2041 cgaaaagtct cacgcccgcc agaatgtgtc gcgtaaggtt atttttgagc aggcgctcga
     2101 tgccgagaag aagggtaatc taaacctcgc tctggagaaa tataaaatgg ctctacaaat
     2161 cgatcaaaaa tttttcgatg cttggctaaa cgctggcgcg atttacacca gacaaggtaa
     2221 aagcaacaaa gcggttgtct gttatcaacg ggcgttggtt tctaaaaacg acgtgcgtgc
     2281 gcattacaat ctcgcggtag agtattacaa aaataacaat tttgtggggg cacaaaaatc
     2341 gctgaagtcg gtcttgcgcc tcgattctcg atacttgaac gcgcatttgc ttttagggta
     2401 tgtttttggc aagttgggag aaaacgataa gtctgaaata tcgattcgta atgcgctaaa
     2461 aatcgatccg caaaatatac aagcgcaaac cgcattggcg ttattgtatt ttcataccga
     2521 cagatcagag ctggcgttga gacaagtgaa tttacttttg tcgcgtagtc ccaacgacaa
     2581 ggtactttta gggttgagcg caaaaattaa tctttcgggc ggcgatttac gcagcgcagc
     2641 agcgggcttc aaaagcattg ccgcacgcga taacgatcta aaacagtttt atact
//
LOCUS       DAOMPD010000367         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15266_length_2687_cov_2.340426, whole genome shotgun sequence.
ACCESSION   DAOMPD010000367 DAOMPD010000000
VERSION     DAOMPD010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15266_length_2687_cov_2.340426"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..>2687
                     /locus_tag="PLY93_11420"
     CDS             147..>2687
                     /locus_tag="PLY93_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRP70132.1"
                     /translation="MIKKIIRFSAENRALTLFLTAVFLLGAWYAAKTIPLDALPDLSD
                     TQVIVYARWDRSPDVIEAQVTTPVVRTLMGGAKVKTIRALTDYGTAFVYVVFEEGTDI
                     YWARSRIVEYMGRIQTLVPADVKIEMAPDATGLGWVYQYVLVDKSGKLDLQKLRALQD
                     FTVKYQLLSVAGVAEVASIGGFQKEYQITLDPIRLRAAKISLAQVVDKIRASNTQAGA
                     RLLEMNGAEYMVRSQGFLKNKADMENIGIGTDDAGNAILLRAVATVTEVPALRRGVVD
                     FMGKGNVVSGIVVMRHGENAHAVIERIKEKITELKKFLPEGVEIQAVYDRSELIEHSI
                     SNLKIKLFEEMLIVALVILVFLSHFPSAIVPILTIPIAVLISFIPLNLLGVSSNIMSL
                     AGIAISIGVLVDGAIVEVENAYRKLEEWQRTGMKEDYHKVRLDALLEVGPSVFFSLLV
                     IAVAFLPIFALVDQEGRMFKPLAYSKNLAMAIAALLAITLDPAMRMLFTRMEPFVATK
                     PLSFLGKLGYKTLNTVVVGKYYPEDKHPISKKLIQLYGPICHYTLRHPKRTIGAAALL
                     MLTIVPLYLKLGHEFFPPLYEESLLYMPVTQPGLSASEAARLLQITDKIIAEQAEVKS
                     VLGKAGRADTATDSAPLSMFETIITLKPRSKWQVKSPEALIQKLDAALKIPGLTNAFT
                     MPIRGRVDMLSTGMRTPLGLKIQARTPADVDRTAIHAEQLLKPMKELRSVIAERATTG
                     YYVDIQQRRLDVARYGLSIDDMQLFVRTALGGEALTQTIEGIERYNVTLRYAADLRNS
                     VEKLKRVPVSLEGSGQVPLSAIADVSLKTEAGMLRNENGFPTGYVYIDT"
BASE COUNT          679 a          700 c          687 g          621 t
ORIGIN      
        1 gagtatgcgt tagtgtcggg tatcgacgca aaaaacgagg tggcactcac gggtgcattc
       61 ctcctcgact ccgaagctaa actcaagtgg ggagacaccc ttccgcagaa tttaaaacca
      121 gcagcgtacg gccacaacgg gcacaaatga taaaaaaaat tattcgattt tcggcagaaa
      181 accgcgcgct cactcttttt ttaaccgctg tgtttttact cggcgcgtgg tatgccgcaa
      241 aaacaatccc actcgatgcg ctgcccgacc tttccgatac acaagtgatt gtgtacgcgc
      301 ggtgggacag gagcccggat gtcatcgaag cgcaagtaac gacgccggtg gtgcgcactc
      361 tcatgggtgg cgcgaaagtg aaaaccatcc gcgcgctcac cgattatggc acggcgtttg
      421 tgtacgttgt gtttgaggaa ggcaccgata tctactgggc gcgctcgcgc attgtggaat
      481 acatggggcg catccaaact ctggtacccg ctgacgtgaa aatagaaatg gcccccgatg
      541 caacaggctt gggttgggtc taccaatacg tactcgtcga caaatcgggt aaactcgatt
      601 tacaaaagtt gcgcgcgctg caagatttta ccgtgaagta ccaactgctc tcagtcgcgg
      661 gtgtcgccga ggtggcgagc attggaggct ttcaaaaaga ataccaaatt accctcgatc
      721 ccattcgatt gcgtgcggca aaaatatccc tcgcccaagt tgtggataaa atccgtgcga
      781 gcaacacaca ggcaggcgca cgccttctgg aaatgaacgg cgccgagtac atggtgcgca
      841 gccaaggttt cttaaaaaat aaagcagata tggaaaatat cggcattggt accgacgatg
      901 cgggcaacgc tatcctcttg cgtgcggtcg ccacagtcac cgaggtgcct gcgttgcggc
      961 gcggtgtagt cgatttcatg ggcaaaggca atgtggtctc gggtattgtg gtgatgcgcc
     1021 acggtgaaaa cgcacatgcg gtgattgagc ggatcaaaga aaaaattacc gagcttaaaa
     1081 aatttctacc cgagggcgtc gaaatccaag cggtgtacga ccgatccgag ctcattgaac
     1141 atagtatttc aaatttgaaa ataaaactct ttgaagagat gctcatcgtc gcgttggtca
     1201 tcctggtttt tttatcgcac tttccttcgg cgattgtgcc catcctcaca ataccgattg
     1261 ccgttttaat ttcatttata ccgctcaatc ttttgggcgt atcgtcgaac atcatgtcgc
     1321 ttgcgggcat cgctatttca atcggcgtgt tggtcgacgg cgccatcgtc gaagtcgaga
     1381 atgcgtaccg taagctcgaa gaatggcaac gcacaggcat gaaagaagac taccacaaag
     1441 tgcgcctcga tgcgcttttg gaagtgggac cgtcggtttt cttttcgttg ctcgtcatcg
     1501 cggtggcgtt tcttcccatt ttcgcgttgg tcgatcaaga agggcggatg ttcaaaccgc
     1561 tcgcgtattc aaaaaactta gcgatggcga tcgccgcgct tttagcgatt acattagacc
     1621 cggcgatgcg tatgcttttt actcggatgg aaccttttgt cgccacaaaa ccattgtcat
     1681 ttttaggaaa gctgggctac aaaacgctca ataccgttgt ggtgggtaaa tattaccccg
     1741 aggacaagca ccccatcagc aaaaaactca tccaacttta tgggcctata tgtcattata
     1801 cgctgcgcca ccccaagcgc accatcggtg cggcggcgtt gctcatgctc acgattgtgc
     1861 cgctctactt aaaactgggg catgaatttt tcccaccgct ctatgaagaa tcgttgctct
     1921 acatgccggt cactcaacca gggctttcgg caagcgaagc ggcacggctt ttgcaaatca
     1981 ccgataagat tatcgccgaa caagctgagg taaaaagtgt tttggggaaa gcaggccgcg
     2041 ccgacaccgc caccgactcc gcgccgcttt cgatgtttga aacgattatt acactcaagc
     2101 cacgctcaaa gtggcaggtg aaatctcccg aagcgttgat acaaaaactc gatgctgcgc
     2161 ttaaaatccc cggactcacc aatgcattca ccatgcccat tcgcggacga gtggatatgc
     2221 tctcgaccgg catgcgcaca cccttgggtc tcaaaataca agcgcgcact cccgccgatg
     2281 tggatagaac ggcaatccac gcagagcaat tactcaaacc catgaaggag ctgcgtagcg
     2341 taatcgccga acgcgcaaca acgggttatt acgtggacat ccaacagcgg cgtcttgatg
     2401 tcgcacgcta tgggctctcc atcgacgaca tgcaactttt tgtacgtacc gctttgggag
     2461 gggaagcgct cacccaaacc atcgaaggca ttgaacgcta caacgttact ctccgttacg
     2521 ctgccgatct gcgcaattcg gtggagaaat taaaacgcgt gccggtatct ttagagggta
     2581 gcggacaagt accgttgagc gcaattgccg atgtatcgct taaaaccgaa gcgggtatgc
     2641 tgcgcaacga aaacggattt cccacgggct atgtctatat cgacact
//
LOCUS       DAOMPD010000368         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15336_length_2677_cov_2.675057, whole genome shotgun sequence.
ACCESSION   DAOMPD010000368 DAOMPD010000000
VERSION     DAOMPD010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15336_length_2677_cov_2.675057"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLY93_11425"
     CDS             <1..648
                     /locus_tag="PLY93_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70133.1"
                     /translation="GSQSFARGFDLSTGSLFWFQDSFPSRSSSNEDILEAMIKEVQRP
                     TTTLSNEQFMAIASEKDRMSSQGASGGLARVAILDFYDRTNSPLYTWLSGSLSSAVDD
                     SMKKIFEYERNDETKSNAAGTKYFKSPADISSSRLKDFQKETNADYIIFGFYSLDKKT
                     GNVVIESKVYDLMKNKAIGGSKSESPVDVRLFNSVDQIAQGIVQDIFTMTQRQNN"
     gene            661..1230
                     /locus_tag="PLY93_11430"
     CDS             661..1230
                     /locus_tag="PLY93_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70134.1"
                     /translation="MKKTTATLMAWILVGGASLFAQSNTTPFYERFKFDVGGFALAPQ
                     SEPAQIGGGASIGYALKSIDIYARIAYLNSDSGLNAIMLPNLLLDYRLPLLTNFFTAT
                     PYVAAGVWYGKIKNSVTHAYGSTPLGTMYLETGMGGELLISGELSLFARVGVGYALAE
                     TTTDSLNSSGLTLTGGLRYSFGSRTTPNY"
     gene            complement(1275..1895)
                     /locus_tag="PLY93_11435"
     CDS             complement(1275..1895)
                     /locus_tag="PLY93_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70135.1"
                     /translation="MGFSLLFPQYAIQYPSPPVTPEVKESTSGSSINKEISGKKDKSA
                     SKTPQAQMRIRIHLGDKSILVAEAPMNATYSFSHQKGRLTYQRTIRAEDIRELAIEAY
                     RARKVSSSKEGDTYEFEPSQVRIELKDGQSFTLSYLFKDLRKIHAKNADGAFTVFGFF
                     ADTYNPKSGWQERGGITMPRVTRQARPAAFTKLEYFEAVDKTGGSE"
     gene            2138..>2677
                     /locus_tag="PLY93_11440"
     CDS             2138..>2677
                     /locus_tag="PLY93_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70136.1"
                     /translation="MASMRKNLNDVYPRVFSRSVERSDPHLFTVTTPENVFQEAQKQN
                     RRLFRYMMNDLMQPGSGIAGYENLKEMGRLADEGKSALILSAHFSNFDVPALYALLYK
                     KGKEGRELFEKIVFIAGRKLTEGPREVKAMAEMFNRVVITAKGHNMTPEEIQIALAIN
                     KASQKAIAKLQREGYVFWLY"
BASE COUNT          737 a          612 c          622 g          706 t
ORIGIN      
        1 ggaagtcaat cgtttgcgcg cgggtttgat ttatctacag gatctttgtt ttggtttcaa
       61 gacagctttc ccagccgctc ttcgtcgaac gaagatattt tagaagcgat gataaaagaa
      121 gtgcaacgac caaccacgac attgagcaac gaacagttta tggcaatcgc ctctgaaaag
      181 gaccgcatgt cttcccaagg ggcatcgggt ggtcttgcac gcgttgcaat tctcgatttt
      241 tacgatcgca ccaactcgcc tttgtacaca tggctgagcg gatcgctctc aagcgcagtc
      301 gacgactcaa tgaaaaaaat cttcgaatat gaacgcaacg acgaaacgaa aagtaatgcg
      361 gcaggcacaa aatactttaa gtcgcccgcc gatatatcgt catcgagact caaagatttt
      421 caaaaagaaa ccaatgccga ttatattatt tttggctttt attcgctcga taaaaaaaca
      481 ggcaatgtag ttattgaatc aaaagtgtac gatctcatga aaaataaagc gattgggggt
      541 tcaaagagcg aatcccccgt cgacgtgcgt ctttttaatt cggtcgatca aatcgcacaa
      601 ggcattgttc aggatatatt tactatgact caacggcaaa acaattaaac ggagataggt
      661 atgaaaaaaa caacagcaac gcttatggcg tggattttgg tcggcggtgc atcgcttttt
      721 gcgcaatcga acactacacc attttacgag cggtttaagt ttgacgtagg cggctttgct
      781 ttagcgccgc aaagcgaacc cgcacaaatt ggcggtggtg cgagcattgg gtatgcgctc
      841 aaaagcatcg atatttacgc gcgcatcgcc tacctcaata gcgactcggg cttgaatgca
      901 atcatgctcc ctaacttgct tttggattat cgcttgccgt tactcacaaa cttttttaca
      961 gcaactccct atgttgccgc cggtgtatgg tatggtaaaa tcaagaattc tgtcacgcat
     1021 gcgtatggta gcactccgtt aggcacaatg tatcttgaaa cggggatggg cggcgagctt
     1081 ctcatcagcg gcgaattgtc gctttttgcg cgcgtcggcg tgggctatgc gttagcagag
     1141 accaccaccg actcgctgaa ttcaagcgga cttactttaa ccggcgggtt gcggtattca
     1201 ttcggtagca gaacaacgcc taattattaa gaacaggctc atcaatctaa atcgtcgacg
     1261 gaaaagcccg ttgattattc gctaccacct gttttatcga ctgcttcaaa atactccaat
     1321 tttgtaaatg cggcggggcg tgcttgccgc gtaacgcggg gcatagttat ccccccgcgc
     1381 tcctgccaac cacttttggg gttgtacgta tcggcaaaaa aaccaaagac ggtaaaagcg
     1441 ccatccgcat tcttcgcgtg gatttttcgt agatctttaa aaagatagga cagcgtaaac
     1501 gactgcccat ctttcaattc aatacgcact tgcgacggtt caaattcata cgtgtcgcct
     1561 tctttgctgg aactcacttt gcgcgcacga tacgcttcga tggcaagttc gcggatgtct
     1621 tcggcacgaa ttgtccgctg gtacgtgagg cggccttttt ggtgtgaaaa cgaatacgtg
     1681 gcgttcatcg gcgcttcagc gacaagaatc gatttatcgc ctaagtgaat gcgtatgcgc
     1741 atttgtgctt gcggggtttt ggaggcggat ttatcttttt taccgcttat ttctttattt
     1801 atcgacgatc cacttgtaga ctcttttacc tcgggcgtta cgggtggtga cgggtattga
     1861 atagcatact gaggaaacag aagagaaaat cccactaaac aaaaaaataa ataccgacta
     1921 aaaaaaggtg aaaacaatga gcaacctctc ttcaatatat atcttcttaa attgggtcgt
     1981 caagacataa tttttctttt ttgttttgaa gagaacttaa agcctgtgcg ccaactcgcg
     2041 cagcgtgtac aaaaacttgt aaaaaaatgt ttcagcgagt tggcgcacag gctccttact
     2101 cactttacaa aatgtcacac acatgctgcg tgtattcatg gcgagtatgc gcaaaaatct
     2161 aaacgatgta tatccccgtg tgttttcgcg gtcggtcgag cgctctgacc ctcatctttt
     2221 tacggtgaca acccctgaaa atgtgtttca agaggcgcaa aagcagaatc gccgcctctt
     2281 ccgctacatg atgaacgatt tgatgcaacc cggctcgggt atcgctggtt atgaaaactt
     2341 gaaagagatg ggtaggcttg ccgatgaagg aaaatcggct ttgatactca gcgcgcactt
     2401 ttctaatttc gatgtccctg cactttacgc actcctctac aaaaaaggta aagaaggacg
     2461 cgaacttttt gaaaaaatag tgtttattgc aggccgcaaa ctcaccgaag ggccacgcga
     2521 agtaaaagcg atggcagaaa tgttcaaccg tgtggtgatt accgcaaaag gccacaacat
     2581 gacgcccgag gaaattcaaa ttgcgttagc tatcaacaaa gcgtcgcaaa aagccatcgc
     2641 taagttacag cgtgagggat atgtgttttg gctctac
//
LOCUS       DAOMPD010000369         2667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15394_length_2667_cov_2.458270, whole genome shotgun sequence.
ACCESSION   DAOMPD010000369 DAOMPD010000000
VERSION     DAOMPD010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15394_length_2667_cov_2.458270"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2555
                     /locus_tag="PLY93_11445"
     CDS             69..2555
                     /locus_tag="PLY93_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70137.1"
                     /translation="MYKRISSFLPISVSIISIFYFPISPAFSAQTSVNASPENKDHAI
                     EILRASEAEYVTVSEGNEGIFILRGGIIVRSGQVILRAETIKMNTKTGEIFGEGRVSF
                     ESGDSKLVGEKFYYDNKHGAGVVYSSKAHADPFYISGSAIKQVENGRYIARDAFFTSC
                     NEKYPHYYFKAKKLWLYKNNEVAAISAVYYVGHTPVFYWPLILKSDFGTGIITQYGNN
                     LTKGSFFQNTWRFSNPFSQDSVWQPRSGKLMFDWYEKTGYLLGINLARNSDVLKYNIN
                     TSIANYKARETTADGSITNQVRQLDGSYGVTNFFWYKIKADIKATLNRSEKNDYATSA
                     YVRFEHYKHRNFELEFGQRFEPDMTINALYRPRFSQQAIQTSLLNWETGLAFTNKDTQ
                     FSVRAERRLLWYQADPDSQSKYAPIYDLVPEISFTHRNFLIRGGDGIFRGLVNTVNLK
                     TQFSQEYKDITSTPIRQFLRNEGYDQVSFYFSLFSWLNFIPSAGLGVQYSYSSQDDAL
                     LQKELKRQSYGYLFTNDTVRLGYPFLYAQAIYTQRYSFGDGAPDLTFGANRMQAVTTS
                     LMSDLNPYASFSLTATRDLRKYPYAITEQERWSDMVARTEANYDFIRGFTVNLFGLSQ
                     RKYNHFNGLGILDTAAYSIQYDTMKTNDFQFYYRVGGYRLPFMRELTLFKITSTYRHN
                     FINIADSSLRLGFETDLLLHSFWRLRFSINSLADRFERYQANSPAHVPFFDDFVQSLN
                     PFDANARRDRILNLESFLVGIEHDLHRWLFRLTFSSQRRTIYTGAALRDRLSFLEQTI
                     YISFTLKDLDGFGLPYTEIYRYNPTDAGIK"
BASE COUNT          698 a          639 c          578 g          752 t
ORIGIN      
        1 taaaactgaa agagcgctta tctcatactt tgtttgacgc tctgtcttcg ccttgcagca
       61 taaaatgcgt gtacaaacgg atttcttctt tccttccgat ctccgtttct attatttcta
      121 ttttttattt tcctatttct cctgcttttt cggcgcagac atcggtgaat gcatccccag
      181 aaaataaaga ccacgcgatt gaaattttgc gggcgtccga agccgagtac gtgaccgttt
      241 ctgaaggcaa cgaggggatt tttattttgc gcggcggtat tattgtgcgc tcaggtcaag
      301 tgattttacg cgccgaaaca atcaaaatga acaccaagac aggggaaatt tttggtgaag
      361 gccgtgtttc gtttgaatca ggggacagta agctcgtcgg agaaaaattt tactacgata
      421 acaagcatgg cgcaggagtt gtatattcct ccaaagcgca cgccgatccg ttttatattt
      481 caggcagcgc catcaagcaa gtcgagaatg ggcgttatat agcgcgcgac gcttttttta
      541 cttcgtgcaa tgagaaatac ccccactact atttcaaagc aaagaaattg tggctctata
      601 aaaataacga agtcgccgca atttcagcgg tttattatgt cggtcacaca ccggtgtttt
      661 actggccgct cattttgaaa tccgacttcg gtacagggat tatcacccaa tatgggaata
      721 atcttactaa aggaagcttt ttccaaaaca cttggcgttt ttctaatcca ttctcacaag
      781 atagcgtatg gcaaccccgt tcgggaaaac tgatgttcga ctggtatgaa aagaccggat
      841 atcttttggg gattaacctt gcgaggaata gcgatgttct taaatacaat ataaatactt
      901 cgatcgccaa ttacaaagcg cgcgaaacca ccgccgatgg ctctatcaca aaccaagtgc
      961 gtcaactcga tgggagttat ggcgtgacca acttcttttg gtataaaatc aaagctgata
     1021 taaaagcgac tttgaatcgc tcggaaaaaa acgattacgc gactagtgct tatgtacgct
     1081 ttgaacacta taagcaccgt aattttgaat tagagtttgg gcaacgtttt gaacccgaca
     1141 tgactatcaa cgcgctctac cgcccccgct ttagtcagca ggcgatacag acaagcttgc
     1201 tcaattggga aacagggctt gcgttcacca ataaagacac gcagttttcg gtccgagccg
     1261 agcggcggtt gttatggtac caagccgacc ccgactctca atcgaaatat gcccctattt
     1321 acgatttagt tccagagatt tctttcaccc accgtaactt tttaatacgg gggggagacg
     1381 gtatttttcg cggcttggtc aacacggtca atttgaagac acaatttagc caggaatata
     1441 aagacatcac atcgacgcct attcggcaat ttttacgcaa cgaaggttac gaccaagtga
     1501 gtttttattt tagccttttc tcatggctca attttattcc ctccgctggg ttgggtgtgc
     1561 agtatagtta cagctcgcaa gacgacgcac tcctccaaaa agaactcaaa cggcaaagct
     1621 acggctatct tttcaccaac gacacggtgc gcttggggta tccattttta tacgcacaag
     1681 cgatttacac acagcggtat tctttcggcg acggtgcgcc cgacctcacg ttcggcgcca
     1741 accgcatgca agcggtcacg acatcgctca tgtccgatct caatccgtat gcgtcgttct
     1801 ctctcacggc aacgcgcgat ttgcgtaaat acccttacgc gattaccgaa caggaacgct
     1861 ggtcggacat ggtggcgcgc accgaagcca attacgattt catacgcggt tttaccgtca
     1921 atttatttgg tttaagccaa cgtaaatata accacttcaa cggtttgggg attctcgata
     1981 ctgccgcgta ttccatccag tacgacacga tgaagaccaa cgatttccag ttttattatc
     2041 gtgtgggtgg gtatcgattg ccttttatgc gagaactcac tctttttaag attacatcca
     2101 cctaccgcca caattttatc aacattgccg attctagttt acgcttagga tttgagaccg
     2161 atttactctt gcatagtttt tggcgtttgc gcttctcgat taactctctt gccgatcgat
     2221 ttgagcgcta ccaagcaaat agccctgcgc acgtaccttt cttcgacgat tttgtgcagt
     2281 cgttaaaccc attcgatgct aacgcacggc gcgatcgtat tttgaattta gaaagttttt
     2341 tggtgggtat cgagcacgat ttacaccgtt ggcttttccg cctcacattc tcttcgcagc
     2401 ggcggactat ttacaccggc gcagcgctgc gcgaccgttt gagtttctta gaacagacaa
     2461 tttatattag ctttactctc aaagatctcg acggcttcgg tttaccatac acagagatct
     2521 accggtataa cccaaccgat gcggggatta aatagcgatt gactctaaat aatttctaaa
     2581 ctaaaatcag gagggaggat ggtgttagta atcgcgccta aactcacaaa accgacgccg
     2641 gtgtctttaa gccgttccgc cgaagtg
//
LOCUS       DAOMPD010000370         2663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15427_length_2663_cov_2.912577, whole genome shotgun sequence.
ACCESSION   DAOMPD010000370 DAOMPD010000000
VERSION     DAOMPD010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15427_length_2663_cov_2.912577"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLY93_11450"
     CDS             <1..724
                     /locus_tag="PLY93_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRP70138.1"
                     /translation="IGNLQSKEVVLAFSNSGETREIVELLPHIKRLQGKLIAVTQKAE
                     STLATSADSAITYSITREGCPLELAPMASTTMSLVIGDAIATALIKLKKFRREDFGKY
                     HPSGTLGRKLLTRVKDVMQTSENILLTRETPFHEILPRMISANLGAMVIVGSKGHVRG
                     ILTDGDIKRCLTQFSKNPEHLFTATAAQIMTVDPVFVYETSLVDEAFALMQEKKTYTL
                     PVVNTAKKPVGIVRMHDLMVGG"
     gene            727..1536
                     /locus_tag="PLY93_11455"
     CDS             727..1536
                     /locus_tag="PLY93_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70139.1"
                     /translation="MADTEKDKVVRAVEKLQRDRIVIFRINALTRVGREELMNIFARL
                     FEEFGLNPALLFNLYSAVVEIIFNAIKANAKHVIFKSEVKQKLVRQKFEDIDALLQII
                     FHEEVLRDFMTRHIVPEKLKRRVNTVLQIEEKKRTGRIDSLKAKDLEVLREFKRLIEQ
                     EEVLIHFTIAMTDERITFNVVNDTPILGKDMRRIEHSRATHRKLFDEGRSTDYFGPDY
                     IDLTESAGFGIAMADEIYYEMGLDPMKHFTIAAENGATRAVMFFPREKFLF"
     gene            1624..2148
                     /locus_tag="PLY93_11460"
     CDS             1624..2148
                     /locus_tag="PLY93_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70140.1"
                     /translation="MFLIHLLFLMLLLTALLVQLVTPLVLNIPMMALILLIFIITIGL
                     FKRARKKKRFPIVASLSLLLQSILFCTSIAIFVGITIPYERPEDSPAVVKGIRRFLVA
                     QGFLENTAIPSASGVEDANRGENPPATINAPEASKIEIKIEDEPAIPKEKAAAGAASN
                     PEPPKLEKSSGEKE"
     gene            2145..2537
                     /locus_tag="PLY93_11465"
     CDS             2145..2537
                     /locus_tag="PLY93_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70141.1"
                     /translation="MKRTLKIILVLYLIILFVLSLLPLTGYYAALGVLPVLSYYLYRK
                     IILKEKNSESIVTRGVFLLACVVFLLGFVLNMFCTLYTTYRMAEFRSTIEEQKKEIYT
                     DIHKEENRKIPPETTTPMPGQSVNIRKK"
BASE COUNT          735 a          588 c          613 g          727 t
ORIGIN      
        1 tatcggtaat ctgcaatcga aagaagtcgt gctcgctttt tccaattcgg gcgaaacgcg
       61 ggaaattgtc gagcttttac cgcatatcaa aagactacaa ggaaagctca ttgcggtgac
      121 tcaaaaagca gagagcacac tcgccacctc tgccgatagc gcgattacgt attccatcac
      181 ccgcgaggga tgcccgcttg aattagcgcc catggcgtcg acaacgatgt cgcttgtcat
      241 tggcgatgcg attgccactg cgcttatcaa gcttaaaaaa ttccgccgcg aagattttgg
      301 aaaataccac ccgtcgggga cgttggggcg caaactcttg acgcgtgtta aagacgtcat
      361 gcagacatcg gaaaacattt tgctgacacg cgaaacgcct ttccatgaaa tactccctcg
      421 tatgatttca gcaaacttgg gtgcgatggt tatcgtcggc agtaaaggcc atgtgcgcgg
      481 tatcttaacc gacggcgaca tcaaacgctg tttaacgcaa tttagtaaaa atccagaaca
      541 tctttttacg gcaaccgcag cgcaaattat gactgtcgat cccgtctttg tgtacgagac
      601 atcgcttgtc gacgaagcgt tcgcgctcat gcaagagaaa aaaacttata cgctgcccgt
      661 tgtaaacacc gccaaaaaac cggtggggat tgttcgtatg catgatttaa tggtgggtgg
      721 ctaattatgg cagatactga aaaagataaa gtagtccgtg cggtagaaaa attacagcgc
      781 gacaggatcg ttatttttcg tatcaatgcg ctcactcgtg tgggacgcga agagctgatg
      841 aatatttttg cacggctttt tgaagagttc ggattaaatc cggcgttgct ttttaatctt
      901 tattctgccg ttgttgaaat tattttcaac gcgattaaag ccaacgcaaa gcacgttatt
      961 ttcaaatccg aggtgaaaca aaaacttgtt cggcaaaaat ttgaagatat agacgcgctt
     1021 ctacaaatta ttttccacga agaagtgttg cgcgatttta tgacgcggca cattgtgcct
     1081 gaaaaactca aaaggcgcgt caacaccgta ttgcaaatcg aggaaaaaaa gcgcacgggg
     1141 cgtattgatt ccttaaaagc aaaagacctt gaagtgttgc gcgaattcaa acgactcatc
     1201 gagcaagaag aagtgttgat ccactttaca atcgcgatga ccgacgaacg catcacgttt
     1261 aacgttgtga acgacactcc tattttgggt aaagacatga ggcgtatcga gcatagccgc
     1321 gctacgcacc gtaaactttt tgacgaaggg cgttctaccg actactttgg tccggattat
     1381 atcgacctca ccgaatcggc ggggttcggt atcgccatgg cggatgaaat ttattacgag
     1441 atgggattgg atcccatgaa gcattttacg attgctgcag aaaatggcgc aacgcgtgcg
     1501 gtgatgtttt tcccgcgtga aaaattttta ttctaatcgt ctatacacgc tgcgcgagtt
     1561 ggcgcacagg ctcctattct ctcgaaagag tagacatgct gcctttactt tttatgcttt
     1621 cctgtgtttt taatccacct tttgtttttg atgcttctct tgactgcact tttggtgcag
     1681 ttggtaaccc cgttggtgct caatataccg atgatggcgc ttattcttct catctttatt
     1741 atcacgattg ggctttttaa gcgagcgcgt aaaaagaaaa gatttcctat cgttgcttcg
     1801 ctttcacttt tgctacaaag tattcttttt tgtactagca ttgccatttt tgtggggata
     1861 actatcccct acgaaaggcc agaagattca cccgcagtgg taaaaggaat tcgtcgtttt
     1921 ctggtggcgc aaggttttct ggaaaacact gccattccaa gcgcatccgg cgtggaagat
     1981 gctaatcgcg gggaaaatcc gcctgcgaca atcaacgcgc ccgaggcgtc caaaattgaa
     2041 attaaaattg aggatgaacc cgctatcccc aaagagaagg cagcggcggg cgcagcgtca
     2101 aaccctgaac cgccgaagct cgaaaagagt agtggagaaa aagagtgaaa agaacgctaa
     2161 aaattatact cgttctttat ttaattattt tatttgttct ttcgctcttg ccgttgacgg
     2221 ggtattacgc cgctttgggt gtacttccgg tcctatcgta ttatctctat cgaaaaatta
     2281 ttttaaaaga gaaaaacagt gaaagtatcg tcacgcgtgg ggtatttttg ctggcgtgtg
     2341 tcgtttttct tttggggttt gtgctcaata tgttttgcac cctatacacc acgtaccgga
     2401 tggcggagtt ccgttcgacc attgaagaac aaaagaaaga aatttatacc gacatacaca
     2461 aagaagaaaa tagaaaaatc ccacccgaaa caacaactcc catgcctggg cagtctgtaa
     2521 atataagaaa gaagtaaatt tattatgata aacttggaat ctcccacctt aaaattttct
     2581 atctccggtt tgcgtggagt ctaccccaac gatatttcac ccgacaaatg ggtcgagtac
     2641 gtgcgcgctt ttgcgcagac gtt
//
LOCUS       DAOMPD010000371         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15458_length_2659_cov_2.187404, whole genome shotgun sequence.
ACCESSION   DAOMPD010000371 DAOMPD010000000
VERSION     DAOMPD010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15458_length_2659_cov_2.187404"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLY93_11470"
     CDS             complement(<1..1050)
                     /locus_tag="PLY93_11470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP70142.1"
                     /translation="MTLAEKLKKKVLEYRKRIHAKGNERMTLLVIPHQTKAMINMQLS
                     NYVIVLAIFSLVFIIIASVFAVGLQRDIQGEADSLQSRDRAFINERELYLNKYHEMAV
                     QQDIFKKQVSNLFTQAKLAENDQTIFLDNEALRESAVQMLDSEGEEYVRSLSYKENRA
                     IKFLDFEATLKAAQNKEQEEGFYFYPEVTLYRQLKLDLRQTENAVAMLKQLLQERNQV
                     QQALPYYWPLVGGRFTSFFGGRISPFGSSQDFHTGIDIANSTGTPIYAAADGRVYGCV
                     YSGGYGLAVRLSHAYGFQTLYGHMSSVLTSCGAFVRKGQQIGRIGATGRATGPHLHYE
                     VRIMEHPINPMPYLTN"
     gene            complement(1047..1565)
                     /locus_tag="PLY93_11475"
     CDS             complement(1047..1565)
                     /locus_tag="PLY93_11475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRP70143.1"
                     /translation="MATIQVHKKYKLHWYEKFYFMSLSLGMWVTLKHFIKSAFLRRMV
                     TVSYPEKKKPLASRFRGLHRLKRNELGQERCTACYCCQWACPADAIQIVAEVVPPERQ
                     YLHPEDKYAKTFQVDLLRCIYCGLCEEACPKGALYLDGGMPMPGDSRDGLILNKDQML
                     EEKGGKIIGERL"
     gene            complement(1565..>2659)
                     /locus_tag="PLY93_11480"
     CDS             complement(1565..>2659)
                     /locus_tag="PLY93_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70144.1"
                     /translation="LEMVARGNDTYITYTAPETEGEKPPYLSHNYQGSLSDICPVGAM
                     LNENTLFQSRVWWYESEDTYCHGCSTLCKVSANVKNNALYRYMPRRLPSPQHAGEDYW
                     GSHDYFICDYGRFSGRDFSKDRLHHYNINGEKSVSQTALPLLAEKVRTAQKILLLGGA
                     TESSEDVDAIQNLVSQWQNGGKTVEWDFRSDAQAFHKDADAQWDFLLSKDARPNARYL
                     RDLGKTIFTDFEQLIQKAKDADLILVLNELSAPFAYQTGVIRPQVKGFNVPNKVEDTA
                     LFSVLNGDKTGASLWGKIALFTTHENMAAQLAALALPVLAFPEKNGVFRDNKGENKNI
                     TASLRPVQGLQSTAAVLQRAFADSEQKVAV"
BASE COUNT          656 a          630 c          634 g          739 t
ORIGIN      
        1 attcgtcaga taaggcatag gattaatcgg atgctccata atacgtactt cgtaatgtaa
       61 atgtgggcca gtggcgcgac cagtggcgcc aatgcgtccg atttgttgac ctttacgtac
      121 gaaagcgccg catgaagtca acacgctact catgtgaccg taaagggtct ggaagccata
      181 cgcgtgcgaa agacgtactg caagaccata gccgccgcta tagacgcaac catacactcg
      241 accatcggca gcggcgtaga tgggcgtacc ggttgagtta gcgatgtcaa tcccggtatg
      301 aaaatcttga gagcttccaa aaggagaaat gcgcccacca aaaaaggaag taaaccgacc
      361 acccacaaga ggccaatagt aagggagcgc ttgttgtact tgattacgct cttgtaggag
      421 ttgtttcagc atcgccaccg cgttttcagt ttgtctgaga tcgagcttaa gttgtctgta
      481 aagcgttacc tctggataaa aataaaatcc ttcttcttgc tctttatttt gtgcggcttt
      541 gagcgtcgct tcaaaatcca agaattttat tgcacgattt tctttgtagc tgagcgagcg
      601 cacatattcc tcgccttcgc tatcgagcat ttgtactgcg ctctcgcgga gagcttcatt
      661 atctaagaaa attgtctgat cgttttccgc taatttagct tgagtaaaaa ggttggaaac
      721 ttgtttctta aaaatgtcct gctgaacagc catctcgtga tacttattta aatagagctc
      781 gcgttcgttg atgaaagcac ggtcgcggct ttgtagtgaa tcggcttcgc cctgaatgtc
      841 acgttgtaac cctacggcaa acaccgatgc gatgataata aaaacaagcg aaaaaattgc
      901 aagtacaatg acgtaattag aaagctgcat gttaatcatc gctttcgttt ggtgtggaat
      961 gacgaggagc gtcattctct cattcccttt ggcgtgtatc cgcttgcgat attcgagaac
     1021 tttctttttt aatttttcag caagggtcaa aggcgctccc cgataatttt accgcctttc
     1081 tcttcgagca tttggtcttt gttgagaatg agtccgtcgc ggctgtcgcc cggcatcggc
     1141 atgcctccgt ctaaatagag cgcaccttta gggcatgcct cttcgcaaag accgcagtag
     1201 atacaccgta ataaatcgac ttgaaatgtt tttgcatatt tatcttcggg gtggagatat
     1261 tggcgttcgg gtgggacgac ctctgccacg atttggattg cgtcggcagg acaagcccac
     1321 tgacagcaat aacacgcggt gcaacgttct tgccccaatt cattgcgctt caaccgatgc
     1381 aaaccgcgaa aacgcgacgc cagtggtttc tttttttcgg ggtatgaaac agtcaccatg
     1441 cggcgcagga acgccgattt aataaaatgt ttcagcgtca cccacatacc caacgagagc
     1501 gacataaaat aaaatttttc gtaccagtgg agtttatatt ttttgtggac ttggattgtt
     1561 gccattagac tgccaccttt tgttcgctat cggcgaaggc gcgttgcaac accgccgccg
     1621 tgctttgtaa gccttgaacc ggccttaacg atgccgttat atttttattc tcgcctttat
     1681 tgtcacggaa gactccattt ttttccggga acgccaacac aggtagcgca agcgctgcga
     1741 gctgcgccgc catgttttca tgcgttgtga aaagcgcaat tttaccccaa agcgatgcgc
     1801 cagttttatc gccattcaac acgctaaaaa gcgcagtatc ttccactttg ttggggacgt
     1861 tgaaaccttt tacctgtgga cggatgacac cggtttggta tgcaaacggc gcacttaact
     1921 cgttcaagac taaaattaaa tcggcgtctt tagctttttg tatgagttgt tcaaaatcgg
     1981 taaatattgt ttttcctaag tctctaaggt atcttgcatt cggacgcgcg tctttactca
     2041 agagaaagtc ccactgcgca tcggcatctt tgtggaacgc ctgagcatcg ctgcggaaat
     2101 cccattcaac cgttttacct ccgttctgcc attggctcac taaattttga atagcatcga
     2161 catcttcact cgattccgtt gcgccaccca ataagagaat tttttgggct gtacgaactt
     2221 tctcggccaa aagggggagc gctgtttggg agacagactt ttcaccgttt atattgtagt
     2281 ggtgcaagcg gtctttagag aaatcacgcc cactaaaacg gccatagtcg cagataaaat
     2341 aatcgtggct accccaatag tcttcaccgg cgtgttgcgg cgaaggcaag cggcggggca
     2401 tataacggta gagcgcgtta tttttgacgt ttgccgaaac tttgcagagc gttgaacaac
     2461 cgtggcagta cgtgtcttcg gattcatacc accacacgcg tgattgaaaa agcgtgtttt
     2521 cgttgagcat cgcccccaca gggcagatat ccgaaagcga accttggtag ttgtggctta
     2581 aatagggtgg cttttcgcct tcggtctcgg gagcggtgta agtgatgtac gtgtcgtttc
     2641 cgcgcgcgac catctctaa
//
LOCUS       DAOMPD010000372         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15507_length_2653_cov_2.290993, whole genome shotgun sequence.
ACCESSION   DAOMPD010000372 DAOMPD010000000
VERSION     DAOMPD010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15507_length_2653_cov_2.290993"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="mraY"
                     /locus_tag="PLY93_11485"
     CDS             <1..697
                     /gene="mraY"
                     /locus_tag="PLY93_11485"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRP70145.1"
                     /translation="FWQIVIATIFAATVFYFPYAPKGGQEQATSLFLPFMKSPLIDMG
                     VVAIFFWLWIVVGTSNAVNLTDGLDGLAAGLSVIVIVTLAALAYVTGLPEVTHYLRIV
                     FVPEANEISVFLAALGGAAVGFLWYNAYPAQVFMGDTGSLALGGAIGMSAILIHREVL
                     LVLLGGIFVMEAMSVILQVGSYKARKKRIFRMAPIHHHFELGGWHENKVVIRFWIVGI
                     LLALISLSSLKII"
     gene            complement(690..1571)
                     /locus_tag="PLY93_11490"
     CDS             complement(690..1571)
                     /locus_tag="PLY93_11490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP70146.1"
                     /translation="MCASPQTAFKYRIERYAFDVWECSACASQFQYPLPKDAYAFYDE
                     GYYTGTAGFHYQDEREKERYHNFVHDARLNRIQEFLSAGRSKILDVGCAFGALVRAAE
                     RHGFEAYGLDVSAFAVREGNKISEEAKNLVRFFQGDLLHLPRGAKKIFAPRSFAAVTL
                     IEVAEHLTKPRAAFETTYNLLHEGGVLVVQTANFEGWQARSAGSNYHYYLPGHLVYYT
                     ATGLKKMLAEIGFREFREFFPVDFSLWAKWRKAWGDVSKAGDMGRFVKMSLYHLKSKF
                     KWQGKPLTSSYVLYAIK"
     gene            complement(1619..2224)
                     /locus_tag="PLY93_11495"
     CDS             complement(1619..2224)
                     /locus_tag="PLY93_11495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP70147.1"
                     /translation="MTNQSVWDAHYTRSRAQQLYPDENVVRLLKKEFGVLTAKSCALD
                     LGAGSGRHLSLLSDHFERVYACDFSEKSLRLYPRRKINATQVKIPELPYLDASFDFIL
                     CWGVLHYLSHEDATATVAEIRRALKANGSIFLTLRSDKDTHLKSQLSTGDLREGYARL
                     FTKEEALSFFDGFSQVKYGFILRQILGEELVVAHHVLWAKR"
     gene            complement(2221..2568)
                     /locus_tag="PLY93_11500"
     CDS             complement(2221..2568)
                     /locus_tag="PLY93_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70148.1"
                     /translation="MDLGKITWLKGDILHYSFNSISDHLRTLESFSTIAAREAFQKGR
                     KVSLVGVFFRSGWVFVQKLFFKWAFLDGAAGWIIAYYSAVSTFSKYIKLYMLHKTPHA
                     LPPLHRVERGRKA"
BASE COUNT          654 a          668 c          605 g          726 t
ORIGIN      
        1 tttttggcaa attgtcattg ccacaatttt tgcggcgact gttttctatt ttccttatgc
       61 ccccaaaggc ggtcaagaac aggcgacgag tcttttccta cctttcatga aaagtccact
      121 catcgacatg ggtgttgtgg caattttctt ttggctgtgg attgtcgtag gcacctcaaa
      181 cgcggtcaat ctcaccgatg ggttagatgg tttggcggcg ggcttgtcgg tgattgtcat
      241 cgtcactctc gctgcgctcg cgtacgtgac cggccttcct gaggtaacgc attatttacg
      301 cattgtgttt gtgcccgaag caaatgaaat ttccgttttc ttggccgcat tgggcggcgc
      361 cgcagtggga ttcctttggt acaatgccta ccccgcacag gttttcatgg gcgacactgg
      421 ctctctggca ttaggtggtg cgattggcat gagtgcaatt ttaatccacc gcgaagtgtt
      481 actcgtgctc ttaggcggta tttttgtcat ggaagcgatg agcgttattt tgcaagtcgg
      541 ctcgtataag gcgcgtaaaa aacgtatctt ccgcatggcg ccgatacatc accatttcga
      601 gttgggggga tggcacgaaa ataaagtggt cattcggttt tggattgtcg gtattttact
      661 cgcgctcatt tcgctatcga gcttaaaaat tatttaattg catagagtac atagctcgat
      721 gtgaggggtt tgccctgcca tttgaatttt gatttcaagt gatagagcga catttttaca
      781 aagcgcccca tatcgcccgc ctttgagaca tccccccatg ctttacgcca ctttgcccac
      841 agcgagaaat ccaccggaaa aaattctctg aactcacgaa agccaatttc agccagcatc
      901 ttttttaagc cagtcgcagt ataatacacc aaatgcccgg ggagataata gtggtaattt
      961 gagcccgcgc tccgtgcttg ccagccttca aaatttgccg tctgcaccac gagtacgccg
     1021 ccttcgtgca ataaattata cgtcgtctca aacgctgctc gcggttttgt taagtgctca
     1081 gcgacttcga tgagagtgac tgccgcaaaa cttctgggcg caaatatttt ttttgcgccg
     1141 cgtggcaaat gcagcaggtc gccttgaaag aaccgaacga gattttttgc ctcttctgaa
     1201 attttattcc cttcgcgcac agcaaaagcc gaaacgtcga gaccgtacgc ctcaaagccg
     1261 tgccgctcgg ctgcgcgcac caacgcaccg aacgcacagc ccacatcaag aatttttgaa
     1321 cgccccgcgc tcaaaaattc ttgaattcga ttcaagcggg cgtcatgcac aaagttgtga
     1381 tagcgttcct tttcccgctc gtcttgatag tgaaaccccg ccgtgccggt ataataacct
     1441 tcatcgtaaa atgcgtaggc gtctttgggg agtgggtatt ggaattgcga agcgcatgca
     1501 ctgcactccc aaacatcgaa cgcatacctt tcgatgcggt acttaaacgc ggtttgaggg
     1561 gatgcgcaca cagggcactc accatctttt ttttttattc ttcgtttgga tttttttatc
     1621 accgctttgc ccaaaggacg tggtgtgcaa ccacaagttc ttcgcctaaa atttggcgca
     1681 aaataaaacc atacttcacc tgtgagaatc cgtcaaaaaa tgagagcgcc tcttctttgg
     1741 taaaaagacg cgcatagcct tctctaagat cgcctgttga aagttgactt ttgagatgcg
     1801 tatctttatc cgaacgcaac gtgagaaaaa tactaccatt ggcttttagc gcccgtcgta
     1861 tctccgcaac agtagcggtg gcatcttcgt gcgataaata atgcaatacc ccccaacaca
     1921 agataaaatc aaaactggcg tcaaggtatg gtaactcggg aatttttact tgcgtcgcat
     1981 taatttttcg gcgtgggtag agccttagac ttttttctga aaaatcgcac gcataaacgc
     2041 gctcaaagtg gtcgctcaaa agcgataaat ggcgcccact ccctgcgcct aaatccaacg
     2101 cacagctttt agccgtgaga actccaaatt cttttttcag caagcgcacc acgttttcat
     2161 cggggtatag ctgctgcgca cgactacgtg tataatgagc atcccaaacg ctttgatttg
     2221 tcacgccttg cggcctctct ctactctatg gagaggcggc aaggcgtgag gcgtcttatg
     2281 cagcatatag agtttgatgt attttgaaaa cgtcgacacc gcagaatagt atgcgataat
     2341 ccatcctgcc gcgccgtcta aaaatgccca tttgaaaaaa agtttttgta cgaacaccca
     2401 ccccgaacga aaaaaaactc ccacaagcga aactttacga cccttttgaa aagcctcccg
     2461 tgcggcaatc gtcgagaaag attctaaggt acgcagatgg tcgctaatcg aattaaacga
     2521 gtaatggaga atatccccct tgagccatgt gattttccct aaatccactt ccacgcggtc
     2581 gtgtggattt tcaccctgcc actttgcatc agtgcggtta aataagtgga ctttcgtgtc
     2641 aggttaccaa ccc
//
LOCUS       DAOMPD010000373         2645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15576_length_2645_cov_2.372201, whole genome shotgun sequence.
ACCESSION   DAOMPD010000373 DAOMPD010000000
VERSION     DAOMPD010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15576_length_2645_cov_2.372201"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..916
                     /locus_tag="PLY93_11505"
     CDS             104..916
                     /locus_tag="PLY93_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRP70149.1"
                     /translation="MQKSPFQFRAVVQARLTSERLPKKVLEKIGDKTIIEHIAARVES
                     IQSAGISLLFAIAEENDSLLSDFLREKKLPFVTGHPSHVLKRFIDAATDLNDDDYVIR
                     LTADNPFIDKDRLAALVERLKERPVDYAYTADLPLGMGSEAIRVAALRSVMLRDTPLR
                     TGGEAGLKDHHREHVTIFIRENPHLYEIFPFRLDSTITPEAAKARVTGIRMTIDEAAD
                     LAVARQVYAHFAARGLSHFGALDVIELSKNDPSMLATNRHVEQRAATSVDTR"
     gene            complement(923..>2645)
                     /locus_tag="PLY93_11510"
     CDS             complement(923..>2645)
                     /locus_tag="PLY93_11510"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP70150.1"
                     /translation="IKKSDNVVVDYGMEVDANFDPGVLQDPDLKARIAELNKFRIPDQ
                     NIVENEYLKDASLEWVSYPYPPIPAVSRATKGLGAANVKYEASSVNYAMPMVFKWQNR
                     LYPSISLLLACRYYGIDVTKDVEVKLGSYVKIKNIPNKKISIGIFGEEKDIMVHPNAD
                     RSVVIPINEQGMMAINFIGGPYTFPHTKMVELAEQVNMKDKTLNTDDYKNKILLTAMY
                     YATGVATTRDIHPSPFSYTTAGIEHHASALNTILMQDFISYAPMWMNFLILFAFAALL
                     GFIIPRTNIGVTLVVVGVMAGLFFVEAVLIFKYVKSVHFFLVPYIEMVVVLIAVIGYK
                     VLTEEENVKYIRNTFSKFVSKDIVNELLANPESLKLGGEKKELTVFFSDIRGFTTMSE
                     SLSPEDLVQLLNDYLSTMTNLVIAYKGTIDKYMGDAIMAFWGAPVQNEDHAYFACLTA
                     VKQLEELKIMQDKWAENGWPVIDIGIGLNSGYAVAGNMGSSHRMDYTVMGDTINLGSR
                     LEGTNKQYGTRIIISEFTYAKVKDRIYARELDDIRVKGKNEPVRIYELIDIVNRDDLD
                     ALRVAVD"
BASE COUNT          689 a          639 c          599 g          718 t
ORIGIN      
        1 tttatacccg acttgaccgc cattggccta ttctaccttc tttctggcat agacaagaac
       61 tttttgattt ctggcgtaca tgtttactaa agtatgacta tccatgcaga aatccccttt
      121 ccagtttaga gcggtagtac aggcccgact cacgagcgaa cgtttgccca aaaaagtgct
      181 tgagaaaatc ggagacaaaa ccatcatcga gcatatcgct gcgcgtgtcg agagtattca
      241 aagcgctggc atttcgcttc tttttgccat cgccgaagaa aacgattctc ttttgtcgga
      301 tttcttacgt gaaaaaaaat taccttttgt gacgggacat ccatcacatg ttttaaaaag
      361 attcatcgac gcagcaacag acttaaacga cgacgattac gttattcgac tcactgcgga
      421 taaccccttt atcgacaaag atcgtttggc tgcgctcgtc gaacgcctca aagaaaggcc
      481 ggtggattac gcgtacaccg ctgatttacc gttggggatg gggagcgagg ccatccgcgt
      541 ggcggcgttg cgttcggtga tgctgcgcga caccccgctc cgtacggggg gcgaggcagg
      601 gctaaaagat caccaccgcg agcatgttac aatttttatc cgagaaaatc cacacctcta
      661 tgaaattttt ccgtttaggc ttgattcaac catcacaccc gaagcggcca aagcgcgcgt
      721 tacaggcatc cgcatgacca tcgacgaagc agcagacctc gcggtggcac ggcaggtgta
      781 cgcccacttt gccgcacgag gcctcagcca cttcggtgcg ctcgatgtga tcgagctttc
      841 taaaaacgac ccgtcgatgc tcgccaccaa ccgccatgta gaacagcgcg ccgctaccag
      901 cgtggatacg cggtagcgga gtttagtcta ccgcaacgcg caacgcatct aagtcgtcgc
      961 ggtttacaat atcaatgagt tcgtaaatcc gcactggctc gttctttccc ttcactcgaa
     1021 tgtcgtcgag ttcgcgtgcg tagatgcggt ctttcacttt tgcgtaagtg aactcagaaa
     1081 taataatccg tgtgccgtat tgcttgttgg taccctcaag acggctgccg aggttaatcg
     1141 tgtcgcccat cacggtataa tccatgcggt gcgatgaacc catgttccct gcaaccgcat
     1201 aacccgagtt aaggccgata ccaatatcga tgacaggcca accgttctcc gcccatttgt
     1261 cttgcattat tttcagctct tcgagctgtt ttacggcggt gaggcaggca aaataggcat
     1321 ggtcttcgtt ttgcactggt gcgccccaaa acgccataat cgcatctccc atgtatttat
     1381 cgatggtgcc tttgtatgca atgaccaagt tcgtcatcgt cgagaggtaa tcattcaaaa
     1441 gttgaaccag gtcttctggg gaaaggcttt cggacatggt ggtgaatcca cggatgtcgg
     1501 agaagaacac ggtgagttct tttttctcac cgcctagttt tagcgattca ggatttgcca
     1561 aaagttcgtt cacaatatct tttgagacga atttggagaa cgtattacgg atatatttca
     1621 cgttctcttc ttcggttaaa actttatagc cgatgacagc aatcagcaca acgaccattt
     1681 caatatacgg caccaagaaa aagtgaacac tttttacgta tttaaagata agtactgctt
     1741 cgacaaaaaa caaccctgcc atgaccccaa caacaaccag agtaactcca atattcgtac
     1801 gggggatgat aaaccctaac aaggcggcaa aggcaaagag aattaagaag ttcatccaca
     1861 tcggtgcata gctaataaag tcttgcatca aaatggtgtt gagcgcactc gcgtggtgtt
     1921 caatacccgc agtggtgtaa ctaaacggtg aagggtgaat atctcgcgtg gttgcaacac
     1981 cggtggcata gtacatcgcc gttaaaagaa ttttattttt atagtcgtcg gtatttaagg
     2041 ttttatcctt catattgact tgctcggcaa gttcgaccat ttttgtgtgt gggaaagtgt
     2101 aaggacctcc aatgaagtta atcgccatca tgccttgttc attgatgggg attaccaccg
     2161 aacggtcggc attaggatgt accataatat ctttttcttc accaaaaata ccaatactaa
     2221 tttttttgtt cggtatattt ttaattttta cataagagcc taacttaact tcgacatctt
     2281 ttgtgacatc gatgccgtag tatcggcaag caagaagcaa ggaaatcgat gggtataaac
     2341 ggttctgcca cttaaaaacc atcggcatgg cataattcac cgacgacgct tcgtatttta
     2401 cgttcgctgc tcccaagcct ttcgttgcgc gactcacagc tggaatggga gggtatgggt
     2461 atgaaaccca ttccaaagat gcgtctttca agtactcgtt ctctacaata ttttggtcgg
     2521 ggattctgaa tttattgagt tcggcaatcc gcgcttttaa gtcaggatct tgtaaaaccc
     2581 ctggatcaaa attggcgtcg acttccatgc catagtccac tacaacgttg tcggattttt
     2641 taatt
//
LOCUS       DAOMPD010000374         2643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15592_length_2643_cov_2.762751, whole genome shotgun sequence.
ACCESSION   DAOMPD010000374 DAOMPD010000000
VERSION     DAOMPD010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15592_length_2643_cov_2.762751"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="ssb"
                     /locus_tag="PLY93_11515"
     CDS             <1..442
                     /gene="ssb"
                     /locus_tag="PLY93_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRP70151.1"
                     /translation="MANDINRVILIGRLTRDPELKSTNSGNYFCRFSLASNRNIYNKQ
                     TGESRDEVGFFDCVAWGKSAESIHKYLQKGRRICVDGSLRWSSWEGQDGKKQSKVEIH
                     IETFQFLDSKQGDGAPRDAAPQEPSNPSAAAFDSGAMSDDDIPF"
     gene            463..759
                     /gene="rpsR"
                     /locus_tag="PLY93_11520"
     CDS             463..759
                     /gene="rpsR"
                     /locus_tag="PLY93_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRP70152.1"
                     /translation="MEERQERQERENTRREEGEHSDRKGGPRGPRFKKKTCRFCENKD
                     LTMEYKRVDILVKLVSNKGKILPRRLTGSCAKHQRDVAKAIKKARNAGFMPFSV"
     gene            764..1804
                     /locus_tag="PLY93_11525"
     CDS             764..1804
                     /locus_tag="PLY93_11525"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRP70153.1"
                     /translation="MELLLGFPLSLVFTLIPLYFFREKIPRKLWLIGVFACTSLFFLL
                     PHAWRLQSFLPGGAALFFLITGVWVLMLNPQNTILKLLGIPLPSRAELKAEIEKVPDV
                     ENRLRKYFTLTEQLKKIWEKRDAFLSGANSVLLLFFTLVSAILSFAMAFQDYQSVGIL
                     KVTIDGVQEQINARLTEAGASAIDIHAQIVGMSPTIIFVLSFFSIYCLGIFLRIFARI
                     RFKQNIIHGQLNLFRLSDSWVWVLIVFAGLYVLGFKNSKLEAIEFYTKNGLWILLFLY
                     MLQGMGIISLFFEVRLLPVNWLIFTLLITSILLPGIFLIFAAIFAVIGLMEVWLGLRK
                     RSLRSINNLENS"
     gene            1804..2250
                     /gene="rplI"
                     /locus_tag="PLY93_11530"
     CDS             1804..2250
                     /gene="rplI"
                     /locus_tag="PLY93_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRP70154.1"
                     /translation="MRVILKQDIATLGRAGDIKEVKDGYARNYLFPRGLVMSATARSV
                     KEKAFLEQVQARKVAKRKKSAEELAAQLNGKDVTVKAKVGEDSKLFGSITNIQVAKEL
                     ETMGFHVERRAIVLDDNIKALGNYKVHIKLHDGVQANIQVHVVAEE"
     gene            complement(2286..>2643)
                     /locus_tag="PLY93_11535"
     CDS             complement(2286..>2643)
                     /locus_tag="PLY93_11535"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70155.1"
                     /translation="RKLAHYPPYVSLARLILIGHAEEIVFREAEKLAKAIEPINTKDL
                     FEKTEISQGTVSLLGPALPQLTKIDNEYRVHFLIKAPSESVLRSYLVRIHPILERFEK
                     SASSRFILDMDPRETS"
BASE COUNT          747 a          538 c          606 g          752 t
ORIGIN      
        1 tatggcaaac gatatcaatc gagttatttt aattgggcgc ttaacccgcg atcccgagct
       61 taaatccacc aactcgggga attatttttg ccgcttttca cttgcttcaa accgcaatat
      121 ctacaacaaa caaaccggcg aaagccgcga cgaagtgggc tttttcgact gcgttgcatg
      181 gggaaaatcc gccgaatcga tacacaagta cctacaaaag ggacggcgta tttgcgtcga
      241 tggttcattg cggtggtctt cgtgggaagg ccaagatggc aaaaaacaga gcaaagttga
      301 aattcacatc gagacattcc agtttctcga cagcaaacag ggtgacggtg caccacggga
      361 tgcagcaccg caagaaccct cgaatcccag cgcagccgcg tttgacagcg gggccatgag
      421 cgacgacgat atcccgtttt aaggaataat tggagaacga caatggaaga acgtcaagaa
      481 agacaagaac gagaaaatac aagaagagaa gagggggagc atagcgaccg caaaggtggc
      541 ccacgcggcc cacgtttcaa gaaaaaaaca tgccgctttt gtgaaaacaa agacctcact
      601 atggaatata agcgggtcga tattttagtg aaattagtct caaacaaagg taaaattcta
      661 ccccgccgct taacgggttc gtgtgcgaaa caccaacgcg atgtggcgaa ggcgataaaa
      721 aaagcacgca acgctggctt tatgccattt agcgtatagt ttgatggagt tattactagg
      781 ttttccttta agcctggtat ttactttaat tccactttat ttctttcgag aaaaaattcc
      841 tcggaagttg tggcttatag gggtgtttgc atgtacatcc ctgtttttcc tattgccgca
      901 cgcatggcgg ttacaatcgt ttttaccggg tggggcagct ctatttttct tgattaccgg
      961 tgtgtgggtt ttgatgctca atccacaaaa tacaatttta aagcttttgg gaatacccct
     1021 gccttcgcgg gcagaactta aagctgaaat cgaaaaagta cccgatgtag aaaatcgcct
     1081 gcgtaaatat tttactctta ccgaacaact taaaaaaatc tgggaaaagc gcgatgcatt
     1141 tctttcggga gctaattcgg tcttattact tttttttact cttgtttcgg ctattttgtc
     1201 ttttgcgatg gcttttcaag attaccagag tgtgggtatc ctaaaggtga caatcgatgg
     1261 ggttcaagag cagatcaacg cacggttaac cgaagcgggg gcttcagcaa tcgatataca
     1321 tgcccaaatt gtcggcatga gtcctacaat tatttttgta ttgagttttt ttagcattta
     1381 ttgcttggga atttttttac gaatttttgc gcgcatccgt ttcaaacaaa atatcataca
     1441 cggacaactc aacctcttcc gtttatccga ttcgtgggtg tgggtattga tcgtttttgc
     1501 gggactttat gtattgggct ttaagaattc aaagcttgaa gcgatagagt tttatacaaa
     1561 aaacggctta tggatattac ttttcctcta tatgttgcaa ggcatgggga ttatttctct
     1621 tttctttgag gtacgtcttt tacctgtcaa ttggttgatt tttactcttt taatcacgag
     1681 tattcttttg ccggggattt ttcttatctt cgccgcaatt tttgccgtga ttggtcttat
     1741 ggaagtttgg ctcggtctac gcaaaaggtc tttacgctct atcaataatt tagaaaatag
     1801 ctaatgcgcg ttattttgaa acaagacatt gcgaccttag gtcgtgccgg tgacattaaa
     1861 gaagtaaaag acgggtatgc gcgtaattac ttattcccac gcggtttggt gatgagcgcc
     1921 acagcgcgct cggtaaaaga aaaagcattt ttagagcaag tacaagcgcg taaagtagca
     1981 aagcgcaaaa aatccgctga agaattggca gcgcaattaa atggtaaaga tgtcactgtc
     2041 aaagctaaag tcggcgaaga cagtaaactt tttggcagca tcactaacat tcaggtggcg
     2101 aaagaactcg aaacaatggg cttccacgtc gagcgccgcg ctattgtttt ggacgacaat
     2161 atcaaagcat tgggtaacta caaagtacat atcaaattgc atgacggcgt tcaagcgaac
     2221 atccaagtac acgttgttgc ggaagaatag acaataaggc gagtaggcta aaacttgcga
     2281 aaccattaac tcgtttctct gggatccatg tctaatataa accgactcga tgcgcttttt
     2341 tcaaaacgct cgagtatggg atggatacgg acaagatagc ttcttaatac actttcactg
     2401 ggcgccttta ttaaaaagtg cactcgatat tcgttatcga tttttgtcag ttgtggtaat
     2461 gctggtccca aaagagaaac cgtcccttga gagatttccg ttttctcaaa caagtctttt
     2521 gtgtttatgg gctcaatcgc tttcgccagt ttttcagcct cacggaaaac aatctcttct
     2581 gcgtggccga taagaatgag acgcgccagt gaaacataag gtgggtagtg agccagtttt
     2641 ctt
//
LOCUS       DAOMPD010000375         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15627_length_2637_cov_2.530984, whole genome shotgun sequence.
ACCESSION   DAOMPD010000375 DAOMPD010000000
VERSION     DAOMPD010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15627_length_2637_cov_2.530984"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLY93_11540"
     CDS             complement(<1..292)
                     /locus_tag="PLY93_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRP70156.1"
                     /translation="MSIEAAYRKVSSPYGFTMYDVEATQALEKASRAAQDLIRAHGFE
                     RIMPAMIDYPETFAEGAHRDIFSIKDNSGENLVLRNDITAQVVKGYVRQIERK"
     gene            complement(289..2382)
                     /gene="glyS"
                     /locus_tag="PLY93_11545"
     CDS             complement(289..2382)
                     /gene="glyS"
                     /locus_tag="PLY93_11545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRP70157.1"
                     /translation="MAEALLFELATEEIPAAYQKNLLSEWQRKLPPLLKATGIPHGGI
                     HVYATARRIAFVVENIHEQGEDITQTLQGPAKSAAFTPDGKPSPALLGFAKKVGIEPT
                     HIEFQDTGKGLYAVATTVRKGALLREALPPLLHTLLTVFRFPRSMRWSDGPTVYARPI
                     AGYFVMYGKAEWDFPHTALKGTVLEVIPRRPVRGHFILDKEPIAVESATAYRAALEKH
                     GIVADVSERRALIERQLKEAAAQKGLHLVENEALLDEVNFIVERPSVLVASFSADYLR
                     LPDGVILSEMNQHQRYFGLREGKKLSNQFLIVTNANTKNTTAAHNIRSGNERVLRARL
                     SDGAFFYDEDLKKPLAERVDDLTQIVFHEGLGTYREKVERMARFAALFDAQSFSTAVL
                     EKSARLAKADLTTQLVFEFDHLQGEIGAQYAAKSGVEAEICTAISEHYQPRFQGDTLP
                     STRLGALLSLADKWDNMLAAFVLGKEPTASQDPFAIRRQALYIIQILAAQKIRVSLAT
                     VIKASLEGYPHAEGVQGVHEKILQFFKARLVTIFEVEGFDKKLTRAAIYTGSDDVCDL
                     FTRAAAIKSIAEKDAASFTALLTAFKRMAHIVGAVKSGRAVDASIFTDPAEKNLHAFA
                     TRLHELSRAPENVSIEYYQKIFTEFAAGKNVVDTFFEKVMVNHDDEKVKQNRIALLLL
                     TLSAVENLIALEELT"
BASE COUNT          644 a          725 c          600 g          668 t
ORIGIN      
        1 ctttgcgttc aatttgccgc acatagcctt tcaccacttg cgcggtgatg tcgttgcgca
       61 acactaaatt ttcgccgcta ttgtctttaa tgctaaaaat atcgcggtgt gcgccttcgg
      121 caaaagtttc ggggtagtca atcatcgcag gcataatgcg ctcgaagcca tgcgcgcgga
      181 ttagatcttg cgcggcgcgg ctggctttct ccaacgcttg cgtcgcctcg acatcgtaca
      241 tcgtgaaacc atacggcgac gaaacttttc ggtatgctgc ttcgatactc atgtcaactc
      301 ttccaatgca atcaaatttt ctactgcgct caacgtcagc aaaagtaatg caatgcggtt
      361 ctgctttact ttttcatcat cgtggttcac cattactttt tcaaaaaaag tatcgaccac
      421 attttttcct gcagcaaact cggtgaaaat tttttggtaa tactcgatag agacattttc
      481 aggcgcccgg ctcagttcgt gcaaacgcgt ggcaaacgca tgaagatttt tttcggcagg
      541 gtcggtaaaa attgatgcgt ccaccgctct cccacttttg accgcaccca caatatgcgc
      601 catccgtttg aacgcagtca gaagcgcggt aaaactcgct gcatcttttt ctgcaatact
      661 cttgattgcc gctgcgcgcg tgaataaatc acacacatcg tccgagccgg tgtagatcgc
      721 cgcacgggtg agcttcttat caaaaccttc tacctcaaaa atagtcacca accgcgcctt
      781 aaaaaattgt aagatttttt cgtgcacgcc ttgcacacct tccgcgtgtg gatacccttc
      841 caacgacgcc ttgatgaccg tggcgagtga aacacggatt ttttgcgcgg ctaaaatttg
      901 aatgatgtac aacgcctgcc ttcgaattgc aaacggatct tggctcgctg tgggctcttt
      961 gcccaacaca aacgcagcga gcatattatc ccacttgtct gctaacgaca aaagcgcgcc
     1021 taaccgcgtc gacggcaatg tatcaccttg gaatcgcggt tgatagtgtt cggaaatcgc
     1081 ggtacaaatc tctgcctcca cgccgctttt ggctgcgtac tgtgcgccaa tttcgccttg
     1141 gagatggtcg aactcgaaca cgagctgtgt ggtgagatcc gccttggcaa ggcgcgccga
     1201 tttttccagc accgcagtcg aaaaactctg cgcgtcaaaa agcgcggcaa aacgcgccat
     1261 ccgctccacc ttttcgcggt acgtccccaa gccttcgtgg aaaacaatct gcgttaaatc
     1321 gtcgacacgc tcggccaatg gttttttcaa atcttcatcg taaaaaaatg caccgtcgga
     1381 aaggcgcgca cgcaacaccc tttcgttacc actgcggatg ttgtgcgccg ctgtggtgtt
     1441 ttttgtgttg gcgttggtca caatcaaaaa ctggtttgat aatttttttc cctcacgcaa
     1501 accgaaataa cgttggtgct ggttcatctc cgataaaatc acgccatcgg gcaaacgtaa
     1561 gtaatccgcg ctaaaagaag ccacaagcac cgacggcctc tcgacaataa aattcacttc
     1621 gtcgagcaac gcctcgtttt ccaccaggtg taatcccttt tgcgccgctg cttctttaag
     1681 ctgccgctcg atgagagcgc gccgctcaga tacatccgca acaatgccgt gcttttccaa
     1741 cgccgctctg tacgcggtag cgctttccac cgcaatcggt tctttatcta aaataaaatg
     1801 cccacgcact ggacgacggg ggattacctc cagcactgtg cctttaagtg cagtgtgggg
     1861 aaaatcccac tcggctttac catacatcac aaagtaaccc gcaattggcc gcgcatacac
     1921 cgtgggacca tctgaccaac gcatcgaacg cggaaaacga aacaccgtca aaagcgtatg
     1981 caagagcggg ggcaatgcct cgcgcaataa agcccccttg cgcacggttg tcgccaccgc
     2041 gtaaaggccc ttacccgtat cttgaaactc tatatgcgtc ggctcgatac ccaccttttt
     2101 cgcaaaacct aaaagcgcgg gggaaggttt gccgtcaggg gtaaacgccg cactttttgc
     2161 aggtccctgc agggtttggg ttatatcttc cccttgctcg tggatatttt caacgacaaa
     2221 cgcgatgcgc cgcgccgtcg catagacatg aataccgccg tgtggaatcc ccgtcgcttt
     2281 caaaagcggc ggtaattttc tttgccattc gctcaataaa tttttctgat acgctgccgg
     2341 tatctcttcg gttgctaatt caaaaaggag tgcttctgcc acttaagcaa atctactctt
     2401 ttatatgcgg cgtaaagcgc aaagagggag tagagtctat gcaccaactc gcgcagcgtg
     2461 tacaaagagg taattttttt gcaaccgttt gtgcactaac tacatctcaa aggtatggca
     2521 aaagaagaaa aagccgtcgg caacgccgac accttgaatg cggcctctac cgaatggcaa
     2581 acgggtaaat ctaaattcgg ttcgtggttt agcggcgata aaatgttcat cctcatg
//
LOCUS       DAOMPD010000376         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15671_length_2630_cov_2.868350, whole genome shotgun sequence.
ACCESSION   DAOMPD010000376 DAOMPD010000000
VERSION     DAOMPD010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15671_length_2630_cov_2.868350"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PLY93_11550"
     CDS             complement(<1..2026)
                     /locus_tag="PLY93_11550"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HRP70158.1"
                     /translation="MNNSILFVAVIFLLSLLIPVDLDARAGGGGSYRSSGSSSSSSSS
                     RSSYSGSSSSSYRSSSSSSYRSSSGSRSSSSSSYRPSKPSYLTIRSFDSVERVLNFQA
                     PGKLVLREKLTAKIPRLSDEPGYELDFSGPQEPWKIDSVRETTGVFDSVREAHHSILT
                     GPKKNAAGTKQLEFFSMASYAATPTLSGSTLVFTSNALCLNAAKCKIELSGLSPLDRV
                     ELSYRDATKGIKEVTTKNFDNVSLQGVYALTIRFAGNHHKSAAQAAWQKAECQFETET
                     HLRMDSSGILSHSENILNPKDTAYICYTLHLSFSTEQFLLKNVKHLLYADSSPLELRL
                     RGNIREFGYEALGAIASGSADAAELTIPLPALYLSTDNYPQISARNITTRKHIFVQTP
                     PDVVASFKLFACENSEENIFPYIWEPPCKTVRPLSFHESRQDNRITLTPDEAQTQKTW
                     LLIGSIQKGAFASPNILTKLQFAFGNYHTIGDYPRWVFWFYFSSIVVGIILAIFIFRY
                     LALRKRRILSQNAENARQTNAERIEDFVTREIQKHDPAFDIEAFKARGRTIAEKIQSA
                     WCAGDMSSCRRYLSQGVYNRFRTQLKIMREIEKKQNAMADFSPSAYVRSGPYDAIAVR
                     LDAKARDTMLDATINPTDALAMAKRAPLTRFSEDYTFMRRHDAQTTQKENA"
     gene            complement(2090..2380)
                     /locus_tag="PLY93_11555"
     CDS             complement(2090..2380)
                     /locus_tag="PLY93_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70159.1"
                     /translation="MGAFALIVCKHEMHGKCFDEKKIHACGEICQHKDDGKACALQTE
                     LGIEQCQKKGDLKICAYMCLFAKDGKDLYCQKQRELCNKPENKSSTDCELIP"
BASE COUNT          652 a          613 c          661 g          704 t
ORIGIN      
        1 cggcgttttc tttctgcgtc gtttgcgcgt cgtggcggcg cataaacgtg tagtcctcgc
       61 tgaaacgggt aagcggtgcc cgtttggcca tcgccaaagc atccgtcgga tttatagttg
      121 cgtcgagcat cgtgtcgcgc gccttggcat cgaggcgtac ggcgatcgca tcgtatggac
      181 cggagcgaac ataggcactc ggggagaaat cggccatggc gttttgcttc ttttcgatct
      241 ctcgcataat tttaagctgt gtgcggaagc ggttatagac cccttgcgaa agataacggc
      301 ggcaactcga catatcgccc gcgcaccatg cggattgaat tttttcggcg atggtacggc
      361 cacgcgcttt gaacgcttcg atgtcgaagg cagggtcgtg cttttggatt tcgcgtgtga
      421 caaagtcttc gattcgttct gcatttgtct gacgcgcgtt ttctgcattt tgcgaaagga
      481 ttcgccgctt tcgcaacgcc agatatctaa agataaatat cgccaaaata atacctacga
      541 caatgctcga aaaatagaac caaaataccc aacgcggata atcaccgata gtatggtagt
      601 taccgaatgc aaattgtaat tttgtcaaaa tattgggtga cgcaaacgcg cctttctgga
      661 tgcttcctat taagagccag gttttttgtg tttgtgcttc atcgggggta agcgttatgc
      721 gattgtcttg tctggattca tggaagctca aagggcgcac ggttttgcaa ggcggctccc
      781 atatatacgg gaaaatattt tcttctgaat tctcgcaggc aaaaagtttg aaactcgcaa
      841 ccacgtctgg tggtgtctgc acaaaaatat gcttgcgcgt tgttatgttc cttgcgctta
      901 tttgcggata gttgtcggta gataaataca aggcgggtag aggtatggtg agttcggcgg
      961 catccgcact gccagaggct atagcgccga gagcttcata gccgaattct cggatattgc
     1021 ctctcaagcg tagctccaac gggcttgaat cagcatagag gaggtgctta acgttcttga
     1081 gcaaaaactg ttctgtagaa aaagaaagat gcaaagtata acaaatataa gccgtatctt
     1141 ttgggttcaa aatattttct gagtgggaaa gaatacccga agagtccatc cgcagatgcg
     1201 tctcggtttc aaattggcat tccgcctttt gccacgccgc ctgtgccgcg ctcttgtggt
     1261 ggttaccggc gaagcgaatc gttagggcat aaacgccttg caaggagaca ttatcaaagt
     1321 tttttgtagt aacttcttta atgcctttag tcgcgtcgcg atacgaaagc tcaactcgat
     1381 cgaggggact aagtccgcta agttcaattt tgcatttggc cgcattcaag cacagtgcat
     1441 tcgatgtgaa tacaagcgta ctacccgaaa gcgtgggagt cgcagcgtac gatgccatag
     1501 aaaaaaactc gagttgtttt gtacccgctg cattcttctt aggccccgtc agaatactgt
     1561 gatgggcctc ccttacagaa tcaaacacac ccgtcgtttc gcgaacgcta tcgattttcc
     1621 atggttcttg agggccagag aaatcgagtt catagccagg ctcatcgctt agacgcggta
     1681 tcttcgccgt caacttttct cgaagtacca atttccctgg agcctggaaa ttcaatacac
     1741 gctcaacaga atcgaagcta cggatcgtta ggtacgaggg tttagacgga cggtatgaac
     1801 tcgatgagct actacgtgac cctgacgaac ttcgatacga cgaagagctc gacgagcggt
     1861 acgaagaact cgatgaaccc gagtaggaac tacgcgaaga gctcgaactt gaagagctcc
     1921 ccgacgaacg gtatgacccc ccgccgccgg cacgggcgtc gaggtctaca ggaataagta
     1981 gcgaaagcag aaaaattact gctacaaaca aaatcgaatt gttcataatg tttctcctga
     2041 gtaaacgcat acgaatgcgc gatgtgtcgg gaaatttgcg cgctaaactc taaggtatta
     2101 actcacaatc tgtgctagac ttgttctctg gtttattgca aagttctctt tgtttttggc
     2161 aatacagatc tttgccgtct ttcgcaaaaa gacacatgta cgcgcaaatc ttcaaatcgc
     2221 cctttttttg gcattgctca atacccagtt cggtttgcag tgcgcatgcc ttgccgtcgt
     2281 ctttatgctg gcaaatttca ccgcaggcgt gaattttttt ctcgtcaaaa cacttgccat
     2341 gcatttcgtg tttacagacg atgagcgcaa atgcgcccat aaaaaacaga aaatatggta
     2401 ctgtacgttt catgcaataa gctatcccgc tggctctgtg cgtcaagaaa aatagatgct
     2461 tttaaataat tggttgacgc ggccaggccg gaacttccgg ttttgcctca ttggagcaaa
     2521 tcgtgctcta cttcgaggca ggcacggcgc atcgcttgag cacagcctcg atggcggcga
     2581 ttagttcgct ttcgtcggcg cgttctttgg cgtggtagta gtaggttgac
//
LOCUS       DAOMPD010000377         2628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15682_length_2628_cov_3.440731, whole genome shotgun sequence.
ACCESSION   DAOMPD010000377 DAOMPD010000000
VERSION     DAOMPD010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15682_length_2628_cov_3.440731"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1121
                     /locus_tag="PLY93_11560"
     CDS             282..1121
                     /locus_tag="PLY93_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70160.1"
                     /translation="MRLFSTYFLLPPLLVRGFILYSFLLCIGISTTSLFAQDSESFFD
                     DDLPEDNSSSDSLKSKPIELRGDDDAKRKAAQQTDEKGQAIEAPKPKITGPTEQVAPM
                     ASRPSETVLDNSRFSLGGNYFMNVGRGISKLPSGFGAFLGYDADESHGRGFDLRLEAG
                     FLNITKGDNSITGGYFEMGPTWIYRPGKFPLQFLTAILPGIGYYDFKDAVSAQNAVKF
                     TAHVALGVEFPFLIKRLDRTDEFVPFIHLRTGMIYDTELPFVQYGVYGGIAYKFGQVI
                     FKY"
     gene            1129..>2628
                     /locus_tag="PLY93_11565"
     CDS             1129..>2628
                     /locus_tag="PLY93_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70161.1"
                     /translation="MKTVLKSQKYIQLSALALVLLFFVAACAKKKKSDDFQNFTGLGY
                     NAVPKTITGGSGLNIAVKGIDINFDGKTDGLYICRTGGIKQDLTPVSAPAFAPTGAGF
                     TDSPTVDFSAMTCSTLPVAHAVVANGRVERVVILNPGAGCSGNQVSITSPVGQSGLGA
                     TATLSLDRGKVVRVTMNSKGAGYVAPTVALAGAGGTGAQMGVNIGHDASGILNNSGQI
                     TGLYLINLGDGRYNATDAGTTPLVATISGDTGTAAPITVSAANLADSELDPDGKCSVT
                     YDPTNADPSKRYIPQMLFTWPVTPTVGLDVNGDTNADYYLYTEEDGSSRVMTNSDGSG
                     SIAKLIIKNPMINESTNWLYQKLDYGQVIGFDILDNSTIANNVLGRIALDREDATYVD
                     AANPMPIISPIRNGVHYAAPMDVNILCADRVACNAVAYSLSNTGSAAIPNFGNVNLDT
                     TGYDYSGAMHAIRPGDSSTISFRQLPFGNYILNYIVRDAAGRESSVQTIT"
BASE COUNT          680 a          616 c          625 g          707 t
ORIGIN      
        1 ttattaacta ctcacattgg acagcaaaac cacaaaattc cagcctaaaa tttggtgagg
       61 aagtcccaat caccccttgg ccgaaggaca aactcgttat tttcatggat atataactcg
      121 cgatgatggc aaggagacaa aaaaattgct tctctataat acgataaagt acgctgcgcg
      181 agttggcgca taggtctcat taaacctcta tttgccagaa ttctcatttt ttggcacacg
      241 tctcgttcac taacgcatat acttatggaa taataccata catgcgccta ttttctacat
      301 attttttact cccgccgctc cttgtccgtg gttttatttt gtattcgttt cttctttgta
      361 taggaatttc taccacttcg ctttttgcac aagattcaga gagctttttt gacgatgatt
      421 tacccgagga taattcaagc tcggatagtt taaaatctaa acccattgaa ttgcggggtg
      481 acgacgatgc caagcgtaag gcagcgcagc aaaccgatga aaaagggcaa gcgattgaag
      541 cacccaagcc taaaataact ggccccaccg aacaggttgc ccccatggcg tcgcgcccca
      601 gcgaaaccgt gctcgacaac tctcgttttt cgttgggggg taactatttc atgaatgtgg
      661 ggcgcggcat cagcaagctg ccctcggggt ttggtgcttt tctgggctac gacgccgatg
      721 aatcgcatgg gcgtgggttt gatttacggt tggaggcggg gtttttgaat ataaccaaag
      781 gcgacaactc aatcaccggc ggctacttcg aaatgggccc aacgtggatt tatcgtccgg
      841 gcaaatttcc tttacagttc ttaacggcaa ttttacccgg cattggttat tatgatttca
      901 aagatgcggt atcggcacag aatgcagtca aatttactgc acacgttgcg ttgggtgtag
      961 agtttccgtt tctaattaaa cggctcgaca gaaccgacga gtttgtgccg tttattcatc
     1021 tgcggacagg tatgatttac gacactgaat tgccgtttgt acaatacggg gtgtacggcg
     1081 gcattgcgta taaatttggg caggttatat ttaagtattg aggcaactat gaaaactgta
     1141 ttaaaatcac aaaaatatat ccaattaagc gcactggcat tagtgcttct ctttttcgtt
     1201 gcagcgtgtg cgaaaaaaaa gaaatccgat gatttccaaa acttcactgg tttaggatat
     1261 aacgctgtcc ccaaaacaat cactggtggt tccggcttaa atattgcagt aaaaggaatc
     1321 gatatcaatt ttgacggcaa gaccgacggc ctttatattt gtcgtactgg tggcatcaaa
     1381 caagatttga cccccgtatc ggctcccgca tttgcaccca ccggcgctgg ttttacagac
     1441 tcgcccaccg ttgatttttc agcaatgaca tgttcgacac tgcctgtggc gcatgcggtc
     1501 gttgccaatg gccgcgttga acgtgtagtc atccttaatc cgggggcagg ttgttcgggt
     1561 aatcaagtct caattacaag ccctgtggga caatctggct taggagcaac agcgacgctc
     1621 tctttagata gagggaaagt cgtacgggta accatgaaca gtaaaggtgc aggttacgtt
     1681 gctccgacag ttgctttagc tggcgcaggt ggcacaggcg ctcaaatggg agtcaacatt
     1741 ggacacgatg caagtggcat tctcaataac tcgggacaaa tcaccggtct ttatctgatt
     1801 aacttgggcg atggtcggta taacgccacg gatgcaggaa cgactcctct cgttgcaacg
     1861 atttcaggcg atacgggcac agctgcgccc ataacagtgt ctgcggcaaa tttggccgat
     1921 tcagaattag accccgacgg taagtgtagt gtgacttacg accctacgaa tgccgatcca
     1981 tctaaacggt atattccaca aatgcttttt acgtggcctg tgacgccgac tgttggtttg
     2041 gacgtcaacg gcgacaccaa cgccgattat tatctataca ccgaagaaga tggttcctcg
     2101 cgtgtgatga ccaactcaga tggctcgggt tcgattgcta aactcattat caaaaaccca
     2161 atgattaacg aatcgacgaa ttggctgtat cagaaactgg attatggcca agtgattggt
     2221 tttgatatat tagataatag caccattgcg aataatgtat taggccgcat cgcactagac
     2281 cgtgaagacg ccacgtatgt cgacgccgcc aatccgatgc ccatcatttc tcccatccgt
     2341 aacggcgtgc actatgccgc accgatggat gtgaacattc tttgtgccga tcgcgtggcg
     2401 tgcaacgccg ttgcgtatag tttaagcaac accggttcgg cggcgatccc caattttggt
     2461 aacgtcaatt tagacaccac tggctacgat tattcgggcg ctatgcacgc gatacgtccg
     2521 ggagacagca gcaccatttc gttccgccag ttgccgtttg gtaattatat attgaattat
     2581 atcgtgcgcg atgcggcggg ccgcgaatca agcgttcaaa ccattact
//
LOCUS       DAOMPD010000378         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15741_length_2621_cov_2.646142, whole genome shotgun sequence.
ACCESSION   DAOMPD010000378 DAOMPD010000000
VERSION     DAOMPD010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15741_length_2621_cov_2.646142"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..202
                     /gene="rpsU"
                     /locus_tag="PLY93_11570"
     CDS             11..202
                     /gene="rpsU"
                     /locus_tag="PLY93_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRP70162.1"
                     /translation="MEVRDSEPVEGAIKKFKREVANSGVLTELKKREFFEKPSVIKRR
                     EREQAERKRFRKRNTYGEA"
     gene            319..>898
                     /locus_tag="PLY93_11575"
     CDS             319..>898
                     /locus_tag="PLY93_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRP70163.1"
                     /translation="MTDADRVIAALPIETYIARVVPLKRKGNNLWGLCPFHGEKTPSF
                     SVSPQKGIYKCFGCGAGGNVITFAKEYNKLSFPEALKLLAEFAGVELTHQPRRLGVDD
                     RNKELLELHLWVHKLYQSCYANSDASAYAAARKVLPETARAFELGYAPNEPRFLETKL
                     NERYRNEPKLLAKAIENLAALGLTGRNDGDSYN"
     assembly_gap    899..917
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <918..2138
                     /locus_tag="PLY93_11580"
     CDS             <918..2138
                     /locus_tag="PLY93_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRP70164.1"
                     /translation="DLRGQVIAFGGRLVREKANAGKYINSPESPLFNKSHTVYRLGDA
                     TQAIRREGFVILCEGYLDALGLFERGLENAVAPLGTAFTADQAKQIKRFTDNVVFFFD
                     NDKAGTDAAFKALQIARAANLAGKVVHPLFSKEKQDPFDLSRSLSNEDMRTVIRDAHQ
                     EVDFITWYFFRYHYETTELAQMKKALREFYDYMRLLETELERDAFLKAAAAVLQIDIK
                     ILQKDFLESLQDKKNTAAWLGAERKPVTEKQPKQVKEVTKQEKEIIALALRFPELMEE
                     EVLLNELPWESDTAYLLFSFFQDRLRIGNTFDWENLSSAMQFLPDALSGLLAAIIMEY
                     EDTLNASVELTVTDAKLNLRRLVRTLVLQTINNRIAMRQKRISELDAQGEDIDELMLE
                     QQEDIDKRIQWQRM"
     gene            2142..>2621
                     /locus_tag="PLY93_11585"
     CDS             2142..>2621
                     /locus_tag="PLY93_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70165.1"
                     /translation="MRWLDVVVLFLYFILLQFVGFWAGRGQKNAADFFSGRHTLPWWV
                     IAASIVAAETSSLTFISIPGVAYNGNLTFMQLAFGYVIGRVVVAYLFLPAYFSGKIDT
                     VYAFLEIRFGVTMRRLAAVLFQLTRVLGTGVRLYATALPLALISGWSIGASILIFALL
                     "
BASE COUNT          671 a          634 c          656 g          641 t
ORIGIN      
        1 ttcgaatgga atcgaagtgc gcgatagcga acccgtcgag ggcgccatta aaaaatttaa
       61 gcgcgaagtt gccaattcag gcgtattgac cgaacttaaa aaaagagaat tttttgaaaa
      121 acccagtgta attaagcgtc gcgaacgcga acaagccgaa cgcaagcgtt ttcgtaaaag
      181 aaacacgtac ggcgaagcgt aacattattc attaaagcta acttaagtac aattttggcc
      241 acacgttgtg gccaaaatta tagatacact gtttagcttt acggttgccg cacaagtttc
      301 tcctcaaaaa aagtccacat gaccgacgcg gatcgtgtta ttgccgcgct gcccattgaa
      361 acctacattg cgcgtgttgt ccccctcaaa cgcaaaggaa ataatctttg gggtctctgc
      421 ccttttcacg gcgaaaagac tccttctttt tcggtgtcac cgcaaaaagg catctataaa
      481 tgttttggtt gcggcgcggg cggcaacgtc atcacgtttg ctaaagagta caataaactt
      541 tctttccccg aagcgttaaa gctcctcgcc gaatttgcgg gcgtcgagct cacgcaccaa
      601 ccgcgcaggt tgggcgtcga cgatcgcaac aaagaattgt tggagctcca cctttgggtg
      661 cataaacttt accaaagctg ttatgccaac tcggacgcca gcgcgtacgc ggcagcacgc
      721 aaagttttac cagagacagc gcgcgctttt gaattaggtt atgcacccaa cgaaccgcgc
      781 tttttagaga cgaagctcaa cgaacgctac cgcaacgagc ccaaactttt agcaaaggcg
      841 attgaaaatc tggccgcgct aggcctcacc ggtcgcaacg acggcgattc gtacaaccnn
      901 nnnnnnnnnn nnnnnnngat ttgcgcgggc aagtgattgc attcggcggg cgtttggtgc
      961 gcgaaaaagc aaacgccggt aaatacatca attcgcccga gtcgccgctc tttaataaat
     1021 cgcacacggt gtaccgattg ggagatgcta cgcaggccat ccgccgtgaa gggtttgtca
     1081 ttctttgcga gggttatctc gacgcgctgg gactttttga gcgcgggctt gaaaatgctg
     1141 ttgcgccgtt gggcactgcg ttcactgccg atcaagcgaa gcaaataaaa cgcttcaccg
     1201 ataacgttgt attttttttc gataacgaca aagcgggcac cgacgctgca ttcaaagcac
     1261 tacaaattgc tcgagcggcc aacttggcag ggaaggtggt gcatccgctt ttctcaaaag
     1321 aaaagcaaga tccttttgat ttgagtcgtt ctctttctaa tgaagacatg cgtaccgtca
     1381 tccgcgacgc acaccaagaa gtcgatttca ttacgtggta tttttttcgt tatcactacg
     1441 agacaaccga gctggcgcaa atgaaaaaag cgctgcgtga gttttatgac tacatgcgcc
     1501 ttttggaaac cgaattggag cgtgatgctt ttttgaaagc ggcggcggcg gtgttgcaaa
     1561 tcgatataaa aatcttacaa aaagatttct tggagtcgct acaagacaaa aaaaacacgg
     1621 cagcatggct tggcgcagaa agaaaaccag ttacagagaa acaacccaag caagttaaag
     1681 aagtaaccaa gcaggaaaaa gaaattatcg cgctggcgtt gcgctttccc gagctcatgg
     1741 aagaagaagt gctcttaaac gagctgccgt gggagagcga caccgcgtat ttactctttt
     1801 ctttttttca agaccggttg cgtatcggca acactttcga ctgggaaaat ctctcgagcg
     1861 caatgcaatt cctacccgac gccttgtcgg ggctactcgc cgcaattatt atggagtacg
     1921 aagatactct caacgcatcc gttgagctta ctgtaaccga tgctaaactc aacctgcgtc
     1981 gcttggtgcg cacgctggta ttgcaaacta tcaacaaccg tatcgccatg cgacaaaaac
     2041 gcatcagcga actggatgcg caaggtgaag acatcgacga actcatgctc gaacaacaag
     2101 aagacatcga caagcgcata cagtggcaac ggatgtagaa aatgcgctgg ttggatgtcg
     2161 ttgttctttt tctttatttt attttactgc agtttgtcgg cttttgggcg gggcgcggtc
     2221 aaaaaaacgc cgccgatttt ttctcaggca ggcacacctt gccttggtgg gtgattgccg
     2281 cttccatcgt cgcggcagaa acgtcgagcc tgacgtttat ttccatcccg ggggtggcgt
     2341 ataacggcaa cctcacgttt atgcagctcg cgttcggcta tgtgattggc cgcgtcgttg
     2401 tggcgtatct ttttttgccc gcgtatttta gcggtaaaat cgatacggtg tacgcgtttt
     2461 tagaaatacg ctttggcgta acgatgaggc gtttggcggc ggtgttgttc caactgacgc
     2521 gggtgttggg caccggcgta cggctttatg caaccgcgct cccattagcg ctcatcagcg
     2581 gatggtctat cggggcgtcg attttgattt tcgcgctgct c
//
LOCUS       DAOMPD010000379         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15755_length_2620_cov_2.597661, whole genome shotgun sequence.
ACCESSION   DAOMPD010000379 DAOMPD010000000
VERSION     DAOMPD010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15755_length_2620_cov_2.597661"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLY93_11590"
     CDS             <1..883
                     /locus_tag="PLY93_11590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP70166.1"
                     /translation="RGITCYRVYDRDIPEYPYTADIYEDHLILTEVATASFIERADSD
                     VIRAEIKKRFATIFHVDINKIHQKTRSKQKGGMQYQKLSEGGAIIAHGNGLQFYIYPD
                     NWLDVGLFLDHRNLRKTVRESAADKKMLNLFCYTGSFSINAGAGGAKLVHSVDLSSRY
                     VKVLTENWRLNADTIAASVLHETFAMDARTFLQESPQNFYDIIVCDAPVFSRSSRQTR
                     DFDVLRDHPQLIRDALRTLSPNGILYFSTNFRKFRIRFPEEGDSAQVKNISAQTIPED
                     FRNKWIHTCYSVQKSAS"
     gene            1352..>2620
                     /locus_tag="PLY93_11595"
     CDS             1352..>2620
                     /locus_tag="PLY93_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRP70167.1"
                     /translation="MVKFRKGALFLLTLFVVSSSLWASEADLKVPDLASQSFLGIDGR
                     TLLTIGLAVCFLGIIFGLVEFFRVKNMPVHKAMREVSELIYETCKTYMITQGKFLSLL
                     WLFVAAIMIYYFGFLAHSTDAAGQVQTGYGFSKVAAILACSVLGILGSYGVAWFGIRI
                     NTFANSRTAFAALAGKPYNVYAIPLRSGMSIGVLLISVALVFMLAILLFMPADYAGAC
                     FIGFAIGESLGASALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCAGDNA
                     GDSVGPTADGFETYGVTAVALISLVLLAVFPQNQVQLLVWIFSISITMIFASLGSYYI
                     NAFIARARYKTADNFNFETPLSSLVWITSIVSVGFTYLVSYLLIANLGDGTIWYKLAS
                     IISCGTLAGAVIPEVVKAFTSTDSAHVREVV"
BASE COUNT          644 a          622 c          601 g          753 t
ORIGIN      
        1 ccgtggcatt acttgctacc gcgtttacga ccgcgacatt cccgaatacc cgtacactgc
       61 cgatatttac gaagaccact tgatattaac cgaggtagcg acggcgtcat ttatagaacg
      121 cgccgacagc gacgtaattc gtgcagaaat aaaaaaacga ttcgctacaa tttttcatgt
      181 tgacataaat aaaattcacc aaaagacgcg ctcaaaacaa aaaggaggaa tgcaatacca
      241 aaaactgagc gagggtggag caattattgc acacgggaat ggtcttcaat tttatatcta
      301 tcccgataac tggctcgatg tggggctctt tctcgaccac cgtaatttac gtaaaacggt
      361 gcgtgagagc gcagcggata aaaaaatgct caatttattt tgctacacag gttcttttag
      421 catcaacgcc ggtgcgggcg gagcaaagct tgtacacagc gttgacttat cgtcgcgcta
      481 cgtaaaagtt ttaaccgaga attggcgatt gaacgcagac accattgccg catctgtgtt
      541 gcatgaaacc tttgcgatgg acgcgcgcac ttttttacaa gaatctccac aaaattttta
      601 cgacattatc gtgtgcgacg cgccggtttt ttcccgtagc tcgcggcaaa cacgcgactt
      661 tgacgtacta cgcgaccacc cgcaactcat ccgcgatgca ctgcgcacac tttcccccaa
      721 cggtatactt tatttttcaa cgaactttcg aaagtttcgt atccgctttc ccgaggaagg
      781 cgattcggct caggtgaaaa atatcagcgc acaaacaatc cccgaagatt ttcgtaacaa
      841 gtggattcat acttgttatt cggtgcaaaa gtctgcgagt tagattaaag gcagaacgcc
      901 aaataactca ctaaagttta gatgtatggc gtgtacttaa aacacttgga aacagcgtaa
      961 cataggagcc ggactggata ttctaaggcg tagtctgttt aacgcacgga catacctgac
     1021 aaatgctaag ccggaaatca gcgacgctag aaggcgagcg gtaagccgtc ttgttctgaa
     1081 attcaatgcc aataatcggt ctgaatggct atattgggct gccattttta caacctgtag
     1141 ctcaatggca tcgctaattt ttcgaggggg ttttcgggcg ccgcgatttt tccgctaacg
     1201 aaaaagcgtc aaatcactgc gcctgaacgc ccccagccag gtgtaaaacg tctgttgtat
     1261 acagagatag ttcctttttt actttacccc ttgtttttga gcaccaatac agccgacact
     1321 ttcttttcat gaaaaatttc gggagaattc tatggtaaaa tttcgtaaag gagcgctctt
     1381 tctgttgacg ctctttgttg tttcatcctc actttgggct agcgaggccg acctcaaagt
     1441 gcctgactta gcgagtcagt cattccttgg catcgacggt cgcacccttt taacgattgg
     1501 gttagcagtc tgttttttag gtatcatttt tggcttggtg gaatttttcc gcgtcaaaaa
     1561 tatgccggtg cataaagcga tgcgcgaggt ttcagaactc atttatgaga cctgtaaaac
     1621 gtacatgata acccaaggta agttcctttc tttattgtgg ctttttgtcg ccgccattat
     1681 gatctattat ttcggctttt tggcgcattc caccgacgcg gcagggcaag tacaaaccgg
     1741 ctatggcttt tcaaaagttg cggcgattct tgcttgttct gtattgggta ttttaggaag
     1801 ctatggcgtc gcgtggttcg gtatccgcat caacacattt gccaactcgc gcactgcgtt
     1861 tgcagcgctc gcgggtaaac catacaacgt ttatgcaatc ccactacggt cggggatgtc
     1921 tatcggggtt cttttaattt ctgttgcctt ggtctttatg ctggcaatcc tcctttttat
     1981 gcccgccgat tacgcgggag cctgcttcat cggtttcgcc atcggtgaat cgttgggtgc
     2041 atcggcgtta cgtatcgccg gtggtatatt cactaaaatt gccgacattg gttccgacct
     2101 catgaaaatc gtttttaaaa ttaaagaaga cgatgcacgt aacccaggtg tgattgccga
     2161 ctgcgcaggg gacaatgccg gtgactcagt gggtcccact gccgacggtt ttgaaacgta
     2221 cggagtcacc gcagtggctc ttatttccct cgtccttttg gcggtattcc cacaaaacca
     2281 agtgcagctc ttggtatgga ttttttccat cagcatcacc atgatttttg cgtcgttggg
     2341 gtcttactac atcaacgcct ttatcgccag agcgcgctat aaaaccgccg acaattttaa
     2401 ttttgaaacc ccgctttcgt cgttagtatg gattacttcc atcgtttcgg tgggtttcac
     2461 ttatttggtg tcttatttgc tcatcgctaa tttaggcgat ggcacaattt ggtataaact
     2521 tgcatcgatt atctcttgcg gtaccctcgc tggtgcagtc atcccggagg tagtgaaagc
     2581 ttttacctcg accgattcag cgcatgtacg cgaagtcgtg
//
LOCUS       DAOMPD010000380         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15770_length_2619_cov_2.152106, whole genome shotgun sequence.
ACCESSION   DAOMPD010000380 DAOMPD010000000
VERSION     DAOMPD010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15770_length_2619_cov_2.152106"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>287
                     /locus_tag="PLY93_11600"
     CDS             <1..>287
                     /locus_tag="PLY93_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70168.1"
                     /translation="EGANAYDVFDYVVAALLHTGFNPEKQSVGIVGLGRIGRKVFAFL
                     NKIGVRTFYFDPFLKNSGSLDAVLECDYVTFHVPLTRTGEHATEGMLNAEY"
     assembly_gap    288..310
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <311..772
                     /locus_tag="PLY93_11605"
     CDS             <311..772
                     /locus_tag="PLY93_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70169.1"
                     /translation="RVQKTIRIIQTCRGQIWSPDFYRSLASHPHIKIAAQDVYPDEPP
                     LASDLLLSQFSTPHIAGYSTRGRLSGILRGVKALFDDIDGTKYFPQSHTWDFNRESEN
                     FQKQVNNFNTLRDTYPWRKEFHEYDAKERAELRVAFPQIKDNFFEHLFEPS"
     gene            845..1489
                     /gene="coaE"
                     /locus_tag="PLY93_11610"
     CDS             845..1489
                     /gene="coaE"
                     /locus_tag="PLY93_11610"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRP70170.1"
                     /translation="MQNAYARVYGLTGVIGSGKSTATQLFKELGATVLDADEIARYVV
                     NPTAPYYAGIRDRITQLFGGLTQGSLFLPNGELNRARLGSVVFGDDQKVRMLNEIMHP
                     CIQQEFAARVNRVDKNSLLIYDVPLLFETGLDKLVKATILVYAPEEVCMQRALERANQ
                     KGEKLNADAIYARLRSQISIEKKRERADYIIDNSGTLESLKDQVERVYQKLVQL"
     gene            1486..2238
                     /locus_tag="PLY93_11615"
     CDS             1486..2238
                     /locus_tag="PLY93_11615"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRP70171.1"
                     /translation="MKEFYVMNLDARRIAILAGGAFVALGITLFIGMSIGRSQSTATQ
                     RLNTESDIAKTEETKLESADKLVLASGPSSVPMKDFNETKNEIPSVPPLPSKREINQD
                     IPLQEDPLVSGPPKTKLHKAHKTTTHSKNKVSGDTKASKKRTHKRSSLADDADEQLAP
                     VAGTAKAKRIGETRYTIQVAAFKQASQANHLIDKLKSEGIRARSEKSGNYFLVTVGRS
                     KSKSKLDKAYAKLKELEYNVYIRKLSPPPDAT"
     gene            2247..>2619
                     /locus_tag="PLY93_11620"
     CDS             2247..>2619
                     /locus_tag="PLY93_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRP70172.1"
                     /translation="MSRSFLSLSALTREEFHALIDRAIAGKHKSSLWGKPLQDKSIAL
                     LFEKPSTRTRISFEVAVHELGGYPLMLTQSDLQTSRGEDLPDTARVMGRYVDGIMARV
                     NSHTTLETFSRYARIPIINGLS"
BASE COUNT          732 a          579 c          631 g          654 t
ORIGIN      
        1 cgaaggggcg aatgcatacg atgtttttga ttacgtggtt gccgctttgc tgcacacagg
       61 atttaacccc gagaagcaaa gcgtgggtat tgtgggtttg ggccgcattg gccgcaaggt
      121 gtttgctttc ttgaacaaaa ttggcgtgcg cactttttat tttgatcctt ttctaaaaaa
      181 tagcggttct ttagacgctg tgctcgagtg cgactatgtc acgttccacg tccctttaac
      241 gcgcacgggt gagcatgcga cagagggtat gcttaacgca gaatactnnn nnnnnnnnnn
      301 nnnnnnnnnn cgcgttcaaa aaacaatccg tattatacaa acttgccgtg gacaaatttg
      361 gtcgcccgat ttttatcgca gtttggcatc gcacccgcat ataaaaattg ctgcgcaaga
      421 tgtgtacccg gatgaacccc cgttggcgag cgacttactg ctttctcaat tttcaacgcc
      481 acacattgcg ggttattcga cgcgtggccg tttgagcggt attctgcgcg gagtgaaggc
      541 gctctttgac gacatcgacg gtacaaaata tttcccgcag tcgcacacgt gggattttaa
      601 ccgcgagagt gaaaattttc agaaacaagt gaacaatttt aacacgctgc gcgatacata
      661 cccctggcgc aaagagttcc atgaatacga tgcaaaagaa cgcgcagagt tgcgtgtagc
      721 ttttccgcaa ataaaggata atttctttga acatctcttt gaaccaagtt agagacaagc
      781 gccaagagaa aatactgtta atttaaggat tgtcgtagcg actctttttt gacctcttct
      841 ctctatgcaa aatgcttatg cgcgcgttta cggtctcact ggcgtcatcg gcagcggcaa
      901 atcgaccgcc actcaattat ttaaagagtt gggagcgact gttctcgacg ccgatgaaat
      961 cgcacggtac gtggtgaatc ccactgcgcc ttattatgca ggtatccgcg ataggattac
     1021 tcaacttttt ggcggactta cacaggggtc gcttttttta cccaacggtg aactcaatcg
     1081 tgcacggttg gggtctgttg tttttggcga cgatcaaaaa gtacggatgc ttaatgaaat
     1141 tatgcacccg tgtattcaac aagaatttgc agcgagggtc aatcgtgtgg ataaaaattc
     1201 tttgcttatt tacgacgtac cgttgctttt tgaaacagga ctcgataaac ttgtcaaagc
     1261 gacaattctg gtgtacgcgc cggaagaagt gtgtatgcag cgcgcactcg agagagcaaa
     1321 ccaaaaggga gaaaaattaa acgccgacgc tatttatgca cgactgcgct ctcaaatttc
     1381 cattgaaaaa aagcgcgaac gcgccgacta tataatagat aactctggta ccttggaaag
     1441 ccttaaagac caagtggaaa gagtgtatca aaagttggta caattatgaa agaattttat
     1501 gtaatgaatt tagacgcgcg gcgcattgcc atcctcgccg gtggagcatt cgtcgcattg
     1561 ggcattacac tttttattgg tatgtcgata ggtcgtagcc aaagcacggc aacgcaaagg
     1621 ctaaataccg aaagcgacat agccaaaacc gaagagacaa agctggaaag tgcggataaa
     1681 cttgttttag cttcagggcc ttcgagtgta cccatgaaag attttaacga aaccaaaaac
     1741 gaaatccctt cggtgccgcc tcttccgagt aaacgtgaaa taaatcaaga tattcctttg
     1801 caagaagatc cgttggtttc agggcctcct aaaacgaaat tgcataaggc gcacaagact
     1861 accactcact caaaaaacaa agtaagcggc gacactaaag cttctaagaa gcgtactcat
     1921 aaacgctcgt ccttggccga cgatgctgat gaacagcttg cgcccgttgc aggcactgca
     1981 aaggcgaagc ggattggcga aacgcgttac acaattcaag tggcggcgtt taagcaagcg
     2041 tcgcaagcaa accatctcat cgataaatta aaaagcgagg ggattcgtgc gcgttctgaa
     2101 aaaagcggta actatttttt ggtaacggtg ggacgttcaa aaagtaaaag caagcttgat
     2161 aaagcgtatg caaaactcaa agaacttgag tataacgtct acatacgtaa gttaagcccg
     2221 ccaccggacg ctacttaatc caacaaatga gccgaagttt tttaagccta agtgccttga
     2281 cgcgtgaaga gttccacgcg ttaatagatc gcgcgattgc ggggaaacac aaatcttcgc
     2341 tttggggtaa acccttgcaa gataaatcaa ttgcgttgtt gtttgaaaaa ccgtcgacgc
     2401 gcacgcgtat ttcgtttgag gtggctgtgc acgaactcgg cggttatccg ttgatgctga
     2461 cgcaaagcga tttacaaact tcgcgcggtg aagatttgcc cgataccgcg cgcgtgatgg
     2521 gacggtacgt cgacggcatt atggcgcgtg tgaacagtca cacaacgctt gaaacttttt
     2581 ctcggtatgc gcgtattcct ataatcaacg ggctatcgg
//
LOCUS       DAOMPD010000381         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15816_length_2613_cov_2.601642, whole genome shotgun sequence.
ACCESSION   DAOMPD010000381 DAOMPD010000000
VERSION     DAOMPD010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15816_length_2613_cov_2.601642"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="PLY93_11625"
     CDS             <1..1721
                     /locus_tag="PLY93_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP70173.1"
                     /translation="IILYWAFRENSYLYYSVTNLAVVLYYIVFDGIATMVLFPESPRF
                     VHSLLSILAMLYAGTYLMFVAQFSQLRAKHPLLDKLLWYFGYTLLFIGIFGHFTIGAR
                     MMNNLTIMISFSAVLVSFIAVLFVDSRNNAALKWFKSGNIFLLLFVLIFLFFLVGYLP
                     SNVFTRNAYRIGTLIELIFFSLALAIRIRTIDDNRRTAEVRAEARMQFAADVSHELRT
                     PLTAIVALTDLMGESQLDTKQTEYLRALKSAAGTMREFANDVLIYAKLEREKVSPERI
                     AFSLHETLQKVADIYEQMARARGITFQRAWEIAPKKLWMGDPLRIGQVLQNLLSNALK
                     FTSTGDKIVLGATVRTIGNKERIEISVRDTGKGISPEKLTDIFGAYVQADASTERHYG
                     GTGLGLSIARQLSELMGGRLDVESTLGFGTVFTMILYLSPTAQQEKVVTVNYKQQVHQ
                     MQKPLRILHVEDQADIRLIFQYFFADTIHHLDSAESGEEGLELFKKHQHDIVFSDVRL
                     PGIDGISVLREIQRIAMERFLPTKTVICSGSLLGNSESLPTPYILIKPISMHDVYATI
                     IHLVNE"
     gene            complement(1738..>2613)
                     /locus_tag="PLY93_11630"
     CDS             complement(1738..>2613)
                     /locus_tag="PLY93_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HRP70174.1"
                     /translation="KKKGARIFFYIPPHRVQRWLGGGQHVKIQLSDAGEAIFGSSNIS
                     HASFAAWNDFSVLLAGEIILEFAESFRTLGFPLERNHLLTLKKLIQNSIPEYNFTYHV
                     FDPNKHQGVLGPLFWRKKNTITEAMVQLFEEARESVFLTSFYFKPTKKLFRAMLRAAR
                     RGVKITIFHSHRAALDATDLAWIAAAVKFDLLLKAKIRIFENKKGEHSKLVLVDGMKA
                     ALGSYNFEDAAHDRLAEAMLVTREKKVVESIQQIFDLLKRNSQNILVKKLWRKRLRFR
                     LRIRIRLWGVFKRWL"
BASE COUNT          695 a          594 c          564 g          760 t
ORIGIN      
        1 acattatcct atattgggca tttcgtgaaa attcatattt atattatagc gtaactaatt
       61 tagcagttgt attatactac atcgtttttg acggcattgc cacaatggtg ctttttcccg
      121 aatcgcctcg atttgtacat tctctgttat ctattttagc aatgctgtac gcaggcactt
      181 atctgatgtt cgttgcccaa ttttctcaac tacgcgctaa acatcctctc ctcgataaac
      241 ttctttggta ttttggctac actcttcttt ttatcggcat atttggccat tttactatcg
      301 gtgcgcgtat gatgaacaat ttaacgataa tgatttcgtt ttctgctgtg cttgtcagtt
      361 ttatcgccgt tctatttgtc gatagccgaa acaatgccgc gcttaaatgg tttaagagcg
      421 gcaacatttt tttattattg tttgttttga tattcttatt ttttttagtt gggtatttac
      481 ccagtaacgt attcacacgc aacgcttatc gtatcggcac actcatcgaa cttattttct
      541 tctcacttgc actggcgata cgaattcgca ccatcgatga taatcgacgc accgccgagg
      601 ttcgtgccga agcgcgtatg caatttgccg ccgacgtttc acacgaatta cgcacaccat
      661 tgacggcgat tgttgcactc accgacctca tgggtgaatc gcagttggat acaaaacaaa
      721 cggaatactt gcgtgcatta aaatcggcag ccggtacgat gcgggaattt gctaacgacg
      781 ttctcatcta cgcaaaactg gaacgcgaga aagtttctcc agaacgcatt gctttttctc
      841 tgcacgagac gctacaaaaa gtagcggata tttacgaaca aatggcgcgc gcacgcggta
      901 tcacttttca aagggcgtgg gaaattgctc ccaagaagtt atggatgggc gatccattac
      961 gcataggtca agtgttgcaa aacctattga gtaacgcgct caagtttaca tccaccgggg
     1021 acaaaattgt tttaggcgca accgtgcgta cgattggcaa caaagagcgc attgaaattt
     1081 cagtgcgcga caccggcaaa ggtatttctc cggagaaact taccgatatt ttcggtgcgt
     1141 atgttcaagc cgatgcaagc accgagcgtc attatggagg tacgggtttg ggattgagta
     1201 tcgcgcggca gttgagcgaa cttatgggtg gtcgcctcga cgttgaaagt acattggggt
     1261 tcggtacagt tttcacgatg atcctttatc tctcacccac agcacaacaa gaaaaagtag
     1321 ttactgtgaa ctacaagcaa caagtgcatc aaatgcaaaa gccattgcgc attttgcacg
     1381 ttgaggatca ggcggatatt cgtctaattt ttcagtattt ttttgccgat acgatacatc
     1441 acctcgatag cgccgaaagt ggtgaagaag gacttgagct ttttaagaag caccaacatg
     1501 atattgtatt ctcggatgtt cgcttgcccg gcatagatgg aatttccgtc ttgagagaaa
     1561 ttcaacgtat cgcgatggaa cgctttttac ctacaaagac tgtaatttgc tcggggagtt
     1621 tgttggggaa cagtgagtcg ctacccacac cgtacatcct cattaagccc atttcaatgc
     1681 acgatgttta tgcaacaatt attcacttgg tcaatgagtg acgaacgtgc agaactttta
     1741 caaccaacgc ttgaacaccc cccaaagacg gatacgtatc cgtaggcgga acctcagacg
     1801 tttcctccaa agttttttga cgaggatgtt ttgagaattt ctcttgagca gatcgaaaat
     1861 ttgttgaatg ctttcaacga cttttttttc gcgcgtgaca agcattgcct cggctaaacg
     1921 gtcgtgcgcg gcgtcctcaa aattataact ccccaaagcg gccttcattc cgtcgacgag
     1981 cactaatttg gagtgctcgc cttttttatt ctcaaagata cgtatttttg ctttgagtaa
     2041 caagtcaaac ttgacagcgg cggcgatcca cgccaaatcg gtggcatcca gcgcggcgcg
     2101 gtgcgagtgg aaaatcgtaa tcttaacccc acgccgagct gcgcgcaaca tagcacgaaa
     2161 aagttttttt gtaggtttga aataaaagct ggtgagaaat acactctcac gcgcttcttc
     2221 aaaaagttga accattgctt cggtgatagt attttttttt ctccaaaaaa gcggccctaa
     2281 aacgccttgg tgcttattag gatcgaacac gtgataagta aaattatatt caggaataga
     2341 attttgaatc aattttttta gcgttaaaag atgatttctc tccagaggaa atcccaacgt
     2401 acggaacgac tcggcaaact ctaaaattat ttctcccgcc aaaagcacag aaaagtcgtt
     2461 ccacgctgca aacgacgcat ggctgatatt gctgctacca aagatggctt cccccgcgtc
     2521 ggagagttga attttaacgt gttgccctcc ccccagccaa cgctgcacac ggtgcggcgg
     2581 tatataaaaa aaaatccgcg ctcctttttt ttt
//
LOCUS       DAOMPD010000382         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15889_length_2604_cov_2.975677, whole genome shotgun sequence.
ACCESSION   DAOMPD010000382 DAOMPD010000000
VERSION     DAOMPD010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_2604_cov_2.975677"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="alr"
                     /locus_tag="PLY93_11635"
     CDS             complement(<1..1059)
                     /gene="alr"
                     /locus_tag="PLY93_11635"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRP70175.1"
                     /translation="MQHYHDNHSALPRTEATPTLKIHRAALANNLDAFKRLAPKSKIL
                     LPVKANAYGCGLGTLMPFFQSSSVDMLGVANVYEGVELRKLGWKKPILNLGGFFAQTL
                     PLFVEHGITPAVTDLWHVEALQNLSHPSIEVHIKLDLGMGRIGIQKQEINNLLERLKS
                     APSIKVKGIFTHFPHTGEKANTHTPQQNKIFIHEAQKIITCLNLIRQDILLHAANSYS
                     TVFFPETHHDMIRPGILFYGYYQSEADRLEFKERFPFKPCIELVATPISYRQIPRGAG
                     ISYSSLYTVEDAIENAAVLPLGYADGFPRALSNNIMFGEHPLRGRVTMDQIIVGNVTE
                     KKTIRLLGEGVPSLEAWGD"
     gene            complement(1059..1520)
                     /locus_tag="PLY93_11640"
     CDS             complement(1059..1520)
                     /locus_tag="PLY93_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRP70176.1"
                     /translation="MLEELLLRSALSMAYNEAEMAFAENEVPVGAVIIYNNEIIARDH
                     NRIVAEANALRHAELLVIERAAKLIKNERLTECHLVTTLEPCMMCSGAVVWSRLASVH
                     FLSEEERSPGLRSLLALPNVNHKPQLYRHRSDEWDAASLMRRFFQMRRTED"
     gene            complement(1528..2223)
                     /locus_tag="PLY93_11645"
     CDS             complement(1528..2223)
                     /locus_tag="PLY93_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70177.1"
                     /translation="MFTNTQLRNALFVSIFLFLSISGVLLLVNPQKAAFALPHAVNTP
                     IIGLEMALSPYEVWNILGDPQTTIGQKIRADFSLGTYIDFGYILAYSLCYLMLTWLLV
                     RRQNIAWGWLLVAALFVVATAISDVLENLAIFRILDTGTEPLVDLHIDQLIVFARLKW
                     LFLGVQGLPAAIMLRREMRRGPSFILTAAFAFGALGIMKQYAPEIMTLFLAFFWVYLF
                     VKLLPLKNRWWPN"
     gene            2424..>2604
                     /locus_tag="PLY93_11650"
     CDS             2424..>2604
                     /locus_tag="PLY93_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70178.1"
                     /translation="MAAIFDDTETFSDTLNAVAYGQIVPLTDKEFAVFQKLIYDWAGI
                     YMNSTKKALVSGRLMK"
BASE COUNT          697 a          593 c          662 g          652 t
ORIGIN      
        1 atcgccccac gcttctaaag atgggacacc ttctcccaaa agtcggattg tttttttctc
       61 ggtaacatta ccgacgataa tttgatccat cgtaacacga ccgcgcaatg ggtgctcgcc
      121 gaacataatg ttatttgaaa gcgcacgggg gaatccatcc gcgtaaccta aaggaagcac
      181 tgcggcattt tcaatcgcat cttccacagt gtaaagcgaa gagtaagaaa tgccggcgcc
      241 acgcggtatt tgtcggtaac taatgggcgt ggccacgagc tcaatacaag gcttgaacgg
      301 aaaacgttct ttgaactcca aacggtcggc ttcgctttga tagtagccat aaaataaaat
      361 ccctgggcgg atcatgtcgt ggtgtgtttc gggaaaaaat accgtactgt acgaattagc
      421 cgcgtgcaag agtatatctt gtcgtatgag atttaggcat gttataattt tttgcgcttc
      481 gtggataaag attttgtttt gttgtggggt gtgcgtattg gctttttctc ccgtgtgcgg
      541 gaaatgagta aagatgcctt ttactttaat cgacggcgca cttttcaagc gctcaagtag
      601 attgttgatt tcctgctttt ggataccgat acgccccata cctaagtcga gtttaatatg
      661 tacttcgata gaggggtgcg ataaattttg taacgcttct acgtgccaca aatcagtgac
      721 agcgggggtg atgccatgct ccacaaaaag aggcagcgtt tgggcaaaaa atccccccaa
      781 gttcaaaatg ggttttttcc aacccagctt acgcaactcg acgccttcgt aaacattcgc
      841 aacgccgagc atatcgacag aggacgattg aaaaaaaggc atgagggtgc ccagaccgca
      901 accatacgcg ttggctttca ccggcagaag aattttactt ttgggggcaa gacgcttgaa
      961 ggcgtcgaga ttgtttgcta acgcagcgcg gtggattttt agtgtcggtg tcgcctctgt
     1021 gcgtggtagt gccgaatggt tgtcgtggta gtgttgcatc aatcttctgt acggcgcatt
     1081 tggaagaagc ggcgcatgag cgatgcggcg tcccattcgt cgctacggtg gcggtaaagc
     1141 tgtggcttgt ggttcacgtt ggggagagct aaaagcgaac gcaaacccgg cgagcgctct
     1201 tcttcggaaa gaaaatgtac cgatgccaaa cgcgaccaca ccaccgcgcc cgagcacatc
     1261 atgcacggtt cgagggttgt gactaagtgg cattcggtga gacgttcgtt tttgatgagt
     1321 ttggcggcac gttcaatcac caaaagttcg gcgtgtcgca gtgcgttcgc ttcggcgacg
     1381 atgcggttgt ggtcgcgcgc aatgatttcg ttgttgtaaa taatcaccgc ccccaccggc
     1441 acttcgtttt cggcgaatgc catctcggct tcgttatacg ccatcgacag tgcggagcgc
     1501 aacaggagtt cttctaacat agacaattca attgggccac caacggtttt taagcggtaa
     1561 cagtttaacg aaaagataaa cccaaaagaa cgctaaaaag agcgtcatga tttcaggtgc
     1621 gtattgtttc atgataccca atgctccgaa tgcaaacgct gcggtaagaa taaacgacgg
     1681 accgcgacgc atttcacgac gcaacataat ggcggctggc aagccttgaa cgcccaagaa
     1741 taaccattta agccgcgcaa aaacaatgag ttgatcgata tgtaaatcga cgagtggttc
     1801 ggtgccggtg tctaaaatac gaaagatggc taagttttca agtacatcgc ttattgctgt
     1861 tgccaccaca aatagcgccg caacaagaag ccatccccat gcaatgtttt gccgcctaac
     1921 aaggagccat gtcaacatta aatagcacaa agagtatgcc agaatgtatc caaagtcgat
     1981 atacgtgcca aggctaaagt ccgcacggat tttttggcca atggtcgttt gtggatcacc
     2041 caagatattc cacacctcgt aagggctcag cgccatttca agcccaatga tgggagtgtt
     2101 gacggcgtgc ggcaatgcga atgcggcttt ctgtggattt accaagagaa gcacgccgct
     2161 tatactaagg aaaagaaaaa tagatacgaa tagagcgttg cgtaattgcg tgttggtaaa
     2221 catgcgtcta agcagggaat caaatctgag tctatgtgcc aaccttaaag tggatctatt
     2281 gaacttatga aatacgtcga cgcgttgtct caaaaatcaa caaagagcct gtgtcccaat
     2341 tcgctgagat aattttttgc aaatttttgt acacgccgcg cgagttggcg cacgggctct
     2401 gaggttgtta ttgaggaaaa aaattggcag caatttttga tgacaccgaa accttctctg
     2461 acaccttaaa cgcagtagcg tacggtcaaa ttgtcccctt gaccgacaag gaatttgccg
     2521 tttttcaaaa actcatctac gactgggcgg ggatctacat gaactcgacc aaaaaagcgc
     2581 tcgtgtcggg gcggctgatg aagc
//
LOCUS       DAOMPD010000383         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15892_length_2604_cov_2.579835, whole genome shotgun sequence.
ACCESSION   DAOMPD010000383 DAOMPD010000000
VERSION     DAOMPD010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_2604_cov_2.579835"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..748
                     /locus_tag="PLY93_11655"
     CDS             92..748
                     /locus_tag="PLY93_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP70179.1"
                     /translation="MAAKKKAAKRKDAQGATRDRKSLKATPKPKKKATAPNSKTGAVD
                     VQYTGAKAHSITPFIMYNANPEEVAKFYVKIFKNSKILRANPMQADFILNGQRFFSYN
                     VGDHAEFKFTWGISFMINVDTQKEVDYYWERLTADGGKESMCGWVQDKFGLSWQVTPN
                     ILLQLVSHKDKAIADRAQQAMLKMKKIDIAALKAAAQGQGRPGADAAKDGVQAAANAK
                     "
     gene            738..1331
                     /locus_tag="PLY93_11660"
     CDS             738..1331
                     /locus_tag="PLY93_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRP70180.1"
                     /translation="MQNKENIVSAGRHPNEVVFTRVFDAPRQMVWDAWTKEENLREWF
                     GPKGSQNIHAKMDFRVGGIFHYSMKFGEHPEMWGLWTFREINPPKSMQLIQNFSDKDR
                     GVTSHPMSPTWPKKMLSTTTFEEAGSKTTLAIHWEPFEATQEEIDTFNAARPNMNDGW
                     SGTFQQLDAYLEQKKIGASHVKSKGTKTRAFARCPSR"
     gene            1345..1716
                     /locus_tag="PLY93_11665"
     CDS             1345..1716
                     /locus_tag="PLY93_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRP70181.1"
                     /translation="MTDRNEMKNWYFTLADFRVEVGFTFQFAGGPSPERQYTHLCEIK
                     EIVPGKKLSYSWRYQGYSGDSLVTFELEAQGSKTLLRFSHTGLDTFPADNPDMSAKNF
                     AAGWNHIVLKALPEYVERKSV"
     gene            1760..2134
                     /locus_tag="PLY93_11670"
     CDS             1760..2134
                     /locus_tag="PLY93_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002724549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRP70182.1"
                     /translation="MNKLTHASGVEEYIAVFPQNIRKILEQLRLTIKQSAPEAVESIA
                     YAMPAYKLNKKPLVYFAGYKNHIGFYATPTGHSAFAKELSRYKQGKGSVQFPLDEPVP
                     LSLIKRIVEFKVKENLKTKKEK"
     gene            2136..2504
                     /locus_tag="PLY93_11675"
     CDS             2136..2504
                     /locus_tag="PLY93_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRP70183.1"
                     /translation="MEIRHKIFTMPFANVYPAYIKKAERKNRSKAEVDAIIFWLTGYD
                     AKSLKHLLAEKVDIETFFAKAPRFNPMAKEITGVVCGVRVEDVEDKLMQKIRYLDKLV
                     DELAKGKAMDKILRQNRLKS"
BASE COUNT          800 a          601 c          659 g          544 t
ORIGIN      
        1 tcgaagccga gacactaaat cctgtcgaaa tgcagcaggg cggctggcag gcaattctcg
       61 acaatttcaa aaaatacgca gaggcaaact aatggcagcg aagaaaaagg cggccaagcg
      121 caaggatgcg caaggggcga cgcgcgacag gaagtcgctc aaggcgacac ccaagccaaa
      181 aaagaaagca accgcgccga attccaaaac tggcgcagtg gatgttcaat ataccggcgc
      241 caaagcgcac agcatcacgc cgttcatcat gtataatgca aaccccgaag aagttgcaaa
      301 gttctacgtg aagatattca aaaactcgaa aatcctacgc gcgaatccga tgcaggcgga
      361 tttcattctg aacggacaga gatttttctc gtataacgtc ggcgaccatg ccgagttcaa
      421 atttacctgg gggatatcgt tcatgattaa tgtcgacacg cagaaagagg tagattatta
      481 ctgggaacgc cttaccgccg acggcggcaa agaaagtatg tgtggctggg tacaagataa
      541 gttcggactt tcgtggcaag taacgccgaa tatattactc caactcgttt cgcataaaga
      601 caaggcgatc gccgaccgcg cacagcaggc gatgttgaaa atgaagaaga tcgatattgc
      661 agccttgaaa gcggctgccc aaggtcaagg acgaccaggg gccgacgctg ccaaggatgg
      721 cgtccaggcg gcggccaatg caaaataagg aaaatattgt gagcgcggga agacatccca
      781 acgaagtcgt cttcacccgc gtcttcgatg cgccccgcca gatggtatgg gatgcctgga
      841 ctaaagaaga aaacctgcgc gagtggtttg ggccgaaagg ctcgcagaac attcacgcga
      901 agatggattt ccgggtgggt gggatttttc attactccat gaagtttggc gagcatcccg
      961 agatgtgggg attgtggaca ttccgcgaaa tcaatccgcc gaagagcatg cagttgattc
     1021 agaatttttc tgataaagac cggggcgtca cgagccaccc catgtccccg acttggccga
     1081 aaaagatgct ctcgacaact acctttgaag aggcaggctc aaagacaacg ctcgcaattc
     1141 actgggagcc gttcgaggcg acgcaggaag agatcgacac gttcaacgcg gcgagaccga
     1201 acatgaacga cggctggagc ggtacgttcc aacaactcga tgcttatctc gagcaaaaaa
     1261 aaataggagc gagccatgtc aaatccaagg gaactaaaac tcgagcgttt gctcgatgcc
     1321 ccagccgata aagtctggcg agctttgacc gaccggaatg agatgaaaaa ctggtatttt
     1381 acccttgcag attttcgtgt cgaggtcggg ttcacttttc aattcgcggg cggcccgtcg
     1441 ccggagcggc agtatactca cctctgcgaa attaaagaga tcgtgcctgg taagaagttg
     1501 tcgtacagct ggcgctacca ggggtacagc ggcgattcgt tagtgacgtt cgagctcgag
     1561 gcgcaaggga gcaagacctt acttagattc tcgcataccg ggctcgatac atttccggcg
     1621 gataaccccg atatgtcggc gaaaaatttt gcggccggtt ggaaccatat cgtactcaag
     1681 gcgttgcccg agtatgtcga gaggaaatcc gtatgacacc aatcaaacaa accgccgaaa
     1741 caaaaggaaa actctaagca tgaataaatt gacgcacgcc agcggcgtcg aagaatacat
     1801 tgcggtcttt cctcaaaata tccgaaaaat tttagagcaa cttcgtttaa cgataaagca
     1861 atcggctccc gaagcggtag agagtattgc atacgcaatg cccgcataca aactgaacaa
     1921 aaaacctctg gtctattttg ccggatacaa aaatcatatc ggattttatg ccacgccgac
     1981 gggacactcc gcgtttgcaa aagaactttc acgatataaa cagggaaaag gttccgtcca
     2041 atttccactg gacgagccgg tacctctgag tttgataaaa cggattgtcg agttcaaggt
     2101 aaaagaaaat ttgaaaacta aaaaagaaaa ataaaatgga aattcgtcac aaaattttta
     2161 caatgccgtt tgcgaacgta taccccgctt atatcaagaa ggccgaaaga aagaaccgtt
     2221 caaaagcgga ggtcgatgcg atcattttct ggctcaccgg atacgacgcc aaaagtttaa
     2281 aacatttgct cgccgagaaa gtagatatcg aaacattttt tgcaaaagct ccgcgattta
     2341 atcctatggc taaagagatc acaggcgttg tgtgcggtgt tcgcgttgaa gatgtcgaag
     2401 acaaactcat gcagaaaatt cgttatttgg ataagctcgt agacgagctt gcaaagggca
     2461 aagcgatgga taaaattttg cgccagaacc gcctgaaatc atgattgcaa aaattgtgac
     2521 actccacatc aactataaat taagaggaca atctaaaccc tcaagacata tgttacactt
     2581 tttataaagt gtttaaggta ggtg
//
LOCUS       DAOMPD010000384         2594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15969_length_2594_cov_2.610477, whole genome shotgun sequence.
ACCESSION   DAOMPD010000384 DAOMPD010000000
VERSION     DAOMPD010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15969_length_2594_cov_2.610477"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLY93_11680"
     CDS             <1..527
                     /locus_tag="PLY93_11680"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804949.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRP70184.1"
                     /translation="YYPRIDLKGNPTDEGVLTIPQAILSSYPKVVGLDGRKMSKSYHN
                     SILLNEPKDLLAKKMMSMKTDTARVHRTDPGNPDNCPVFSYYDFFAPDLKAEVDKGCR
                     SATLGCVDCKKQIIARMEKTIAPFREKRAELKNDEKKIAEILAAGAEKARRVAKITMA
                     RVKDALGMSAPYKL"
     gene            527..1384
                     /locus_tag="PLY93_11685"
     CDS             527..1384
                     /locus_tag="PLY93_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRP70185.1"
                     /translation="MQHETTTATLSLERVDISTFQVAFETRTGSTEKGPLAALWRLIE
                     SYEVDIFVVSLSRITADFLTYMQSSAISLDEQADFTQMAARLIYYKSRQLLPNAAAAE
                     DTAPDTLPYELVEQLLEYKKMQQAAEQMRTLEEKSQLRFARDSQWNHFEKDLDYLQVD
                     LLSFLKAFRDFLEREEKSRPMQIADEHISVEEMIPYMASRIVGESEASFFRSVTGFSI
                     ARIVACFLALLEMAKQGLVALRQDVWLGDIHFIHATDGAQLSLAFENTEETAPRETAP
                     YEQSSNLTP"
     gene            1381..1920
                     /gene="scpB"
                     /locus_tag="PLY93_11690"
     CDS             1381..1920
                     /gene="scpB"
                     /locus_tag="PLY93_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRP70186.1"
                     /translation="MSDTQYLKASIEGLLFLSGHGLTFSEIQSGLSENAEQIKIALDS
                     LIDEYAEREAGIAIQEISGRYQFTTQPHIYPAIQNFLRQKKKETLSKSTLETLAIITY
                     KQPITLPEIEEIRSVSSRTQLLTLQQKKLIKSLGQKETVGKPTMYGTTKEFLRYFGLN
                     SLDELPAPQDVKELNFDEL"
     gene            1945..>2594
                     /gene="pheA"
                     /locus_tag="PLY93_11695"
     CDS             1945..>2594
                     /gene="pheA"
                     /locus_tag="PLY93_11695"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRP70187.1"
                     /translation="MKEWRDKIDSIDADIVRLISERATYASEIAKAKAKNGIPLFQPD
                     REQEVYTKIARLNSGALDDTAFKNIYREIMSATLKLEGSLNVGYFGVEGSFTHQAALD
                     KFGRSLPLHSYRTIDDVFQAVEAREIKYGIVPIENSTEGMVKATLDALSKYDLHIYSD
                     VLLQIKQSLLSTAKDVSQIEKVFTHPQAYAQTQNYLMKHLRRAEWIETYSTSEAAER"
BASE COUNT          760 a          653 c          589 g          592 t
ORIGIN      
        1 attactatcc ccgtatcgac ctaaaaggaa atcctactga cgagggagta ctcacaattc
       61 cgcaagcgat tttgtcgtcg taccccaaag tcgttggttt agacgggcgc aaaatgagca
      121 agagttatca caattctatt ttgctcaacg agcctaaaga cttgctcgcg aagaaaatga
      181 tgtcgatgaa aacagacacc gctcgggtgc accgcaccga tcctggaaat cccgacaact
      241 gccctgtctt tagctactac gattttttcg cgcccgactt aaaagccgaa gtggataaag
      301 gttgtcgcag tgcaactttg ggttgtgtgg actgcaaaaa gcaaatcatc gcgcgtatgg
      361 aaaaaacaat cgccccattt cgtgaaaaac gcgcggaact caaaaacgat gaaaagaaaa
      421 ttgctgaaat tttagccgcc ggcgctgaga aagcgcgtcg tgtcgcaaaa ataaccatgg
      481 cgcgcgtgaa agacgcgctc ggtatgtcgg cgccctataa actttaatgc agcacgagac
      541 gaccaccgcc acgttatcgc ttgaaagagt tgatatatcc acttttcaag tggcgtttga
      601 aacgcgcacc ggctcaaccg agaaagggcc tttggcggct ctgtggcgtc tcatcgaaag
      661 ttatgaagtc gatatttttg tagtttctct ttcgcgcatc accgccgatt ttttaacgta
      721 tatgcaaagc tcggcgattt cactcgacga gcaggcagat ttcactcaaa tggcggcgcg
      781 gttgatttac tataaatctc gccaactttt acccaacgcc gccgccgccg aggacactgc
      841 ccccgacacg ctgccgtatg aattggtgga gcaactttta gaatataaaa aaatgcaaca
      901 agccgccgag caaatgcgta ccctcgaaga gaaaagccaa ttacgctttg cacgcgattc
      961 gcagtggaat cattttgaaa aagatttaga ttatttacaa gtcgatttat tatccttttt
     1021 gaaagcattc cgcgattttt tagagcgcga ggaaaaatcg cgccccatgc aaatcgccga
     1081 cgaacatatc agcgtcgaag aaatgatccc ttacatggcg tcgcgcatcg tgggtgaaag
     1141 cgaagcatct ttttttcgct cggtaacggg gtttagcatt gcgcgcatcg tcgcttgttt
     1201 tttagcgctc ctcgaaatgg cgaaacaagg actggtggca ttgcggcaag atgtatggtt
     1261 gggcgacatt catttcatcc acgccaccga cggcgctcaa ctttcactgg cgtttgagaa
     1321 cacagaagaa acagcgccgc gtgaaaccgc tccgtacgaa caatcgagca atcttacacc
     1381 atgagcgata cacaatactt aaaagcttct atcgaagggt tgctctttct ttcagggcac
     1441 ggcctcacct tttctgaaat ccagtcggga ctttcagaaa acgcagaaca gataaaaatt
     1501 gcactcgact cgctcatcga cgagtacgcc gagcgcgaag cgggcatcgc catccaagaa
     1561 atttcggggc gttaccaatt caccacgcaa ccgcatattt accccgcaat ccaaaatttt
     1621 ttacggcaaa agaaaaaaga gacgctgagt aaatcaacct tagagacgct ggcaatcatc
     1681 acgtacaagc agcccatcac gcttccagaa atcgaagaaa tccgcagcgt ctcgtcgcgt
     1741 actcaacttt tgaccctcca gcaaaaaaaa ctcattaaat cgttgggaca aaaagaaacc
     1801 gtcggcaaac cgacgatgta cggcacgacc aaagagtttt tacgctattt tggattgaat
     1861 agcttggacg aactacccgc accgcaggac gtgaaggagc taaactttga tgaactctaa
     1921 aatttttata gcggagcaca accgatgaaa gagtggcgcg ataaaatcga cagcatcgac
     1981 gccgacattg tacgcctcat ttcggagaga gcaacgtacg cttctgaaat tgcgaaagca
     2041 aaagccaaaa acggcattcc ccttttccaa cccgaccgcg aacaagaagt ctacactaaa
     2101 attgcgcgcc tcaactccgg tgcgttagac gatacggcat tcaagaatat ctatcgtgaa
     2161 attatgagcg cgaccctcaa actggagggt tcgctgaacg tcggttattt tggtgtcgag
     2221 ggttcattta ctcaccaagc agcgctggat aaatttggcc gcagcttacc gctccattcg
     2281 taccgtacca tcgacgatgt gtttcaagcg gtggaagcgc gtgaaatcaa atacggtatc
     2341 gtaccgattg aaaactcgac cgaagggatg gtcaaagcga cgctcgatgc gttgagtaaa
     2401 tacgatctcc atatttactc cgatgtatta ttgcaaatta aacaaagcct tttgagtacc
     2461 gcgaaagatg tatcccaaat tgaaaaagtt ttcacccacc cgcaagcgta cgcgcaaaca
     2521 caaaattatt tgatgaagca cttgcgccgc gccgaatgga ttgaaacata ctccacctcc
     2581 gaagccgcag aacg
//
LOCUS       DAOMPD010000385         2592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15984_length_2592_cov_3.178557, whole genome shotgun sequence.
ACCESSION   DAOMPD010000385 DAOMPD010000000
VERSION     DAOMPD010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15984_length_2592_cov_3.178557"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLY93_11700"
     CDS             complement(<1..867)
                     /locus_tag="PLY93_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRP70188.1"
                     /translation="MNTENLSAKSNRRKKILGLIVCLSIFTAATVGQIESKAQKEISD
                     EGLTELFNAALFHVRNDYVDEVSRQTLIFGAIRGMLASLDDAHTRFMTQDETNELQTE
                     MRGNFGGLGIEISQRDNVLTVVSPIDDTPAMRAGIRPNDKIVEIDKKSTRDLSLTDAV
                     KQLRGKPGTSVNITVIRDGEDEALSFDLVREIIKIQVVTSQFLEKEKLGYVRLKQFNQ
                     TATEDLARVLTEFKKKKAKGIILDLRWSPGGLLDAAHRIGNFFIKSGVIVSTRGRKKE
                     LDRVFNAEPSAAI"
     gene            complement(916..>2592)
                     /locus_tag="PLY93_11705"
     CDS             complement(916..>2592)
                     /locus_tag="PLY93_11705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP70189.1"
                     /translation="IEFKEISLSTVRSKVLFFISIMIATLFVANFMIYYNVSFSVLMR
                     FSLFAIAIAAVMAFMLFSVILQALREIEQTAQGIREGKNTMLFPEALDAEFINVATGL
                     NTATQTIRDYQHNLERKVEMRTAELTEANEALHQKDHLIQMELDFAAEIQKGIIPTKI
                     EEWNGLKFFSYYLPMEKVSGDYFDVFPAHGNRLGVLMADVSGHGVPAALITTMAKVAF
                     ARAAQNTHSPVQTFREVNNQLLEIVTTQDYLTAFYLTIDDTHHFLFGNASHQHAKIAR
                     ARSVSTEETTDTEESSISTIDSLDTDGLFVGAMHEANESYAEKEGRLYAGDRLFLYTD
                     GLIEIRNKAGEELGNAQFDALLQKASSLPAEAVVPFVVDEVLKFAEGNPPNDDISMLM
                     IEVNPEYSRFLERVARANRLIESNNSQEGVELLEEAIQIYPKNLPALKTAALVNFDLA
                     RIQKSERYLKMYLEINKQDAEIFYYLSSIAILRKNFMNAEKYAREAIHLRANYALAFN
                     NLAIASLNVGKYVAARHAMEKALSLEPENSEIQKNAEHLEKYIEGQNFNS"
BASE COUNT          648 a          609 c          605 g          730 t
ORIGIN      
        1 aatcgccgcc gacggttccg cattaaaaac tcgatcgagt tcttttttac ggccacgtgt
       61 cgatacaatc acacccgact taataaagaa gttaccgatg cggtgcgcag cgtctaagag
      121 accgcctggg ctccaacgta aatcgagaat aatgcctttt gcttttttct ttttaaactc
      181 agtcaaaacc cgcgccaaat cttcggtcgc cgtctggtta aactgcttta accgcacata
      241 gcccaatttt tctttttcta aaaattgcga agtgacgact tgaattttaa tgatttcacg
      301 cactaaatcg aaagagagcg cttcgtcttc accatcgcgg atgactgtga tattcaccga
      361 ggtgcccggt ttaccacgca actgttttac tgcgtctgtg agagaaagat cgcgcgtcga
      421 ctttttgtct atttccacaa ttttatcgtt ggggcgaata cccgcacgca tcgcaggggt
      481 gtcgtcaatg ggagaaacca cggtgagcac gttgtcgcgt tgcgagattt caatgcccaa
      541 gccgccaaag ttaccgcgca tttcggtttg caactcgttg gtttcgtctt gagtcatgaa
      601 gcgagtgtgt gcatcgtcta aagaggcaag catacctcga atcgcgccaa aaattaaggt
      661 ttgtcgagaa acctcgtcga cgtagtcgtt gcgtacgtga aaaagcgctg cgttaaaaag
      721 ttcggtgagg ccctcatctg aaatttcttt ctgagctttg ctctcaattt gcccaacggt
      781 cgccgctgtg aaaatcgaca aacaaacgat taagccgagg atttttttgc ggcggttact
      841 tttcgccgat aagttttctg tgttcatgga agattcaagg ttggcatcgg ttcgttggat
      901 tatcggtcaa tcattttacg agttaaaatt ttgtccttct atatactttt ccaaatgctc
      961 tgcatttttt tgtatttcgc tattttctgg ttccagagaa agcgccttct ccatggcgtg
     1021 ccttgccgca acgtacttgc cgacgttgag cgaagcaatc gccagattat taaaggccag
     1081 cgcataattt gcgcgtaagt ggatcgcttc acgtgcgtac ttctctgcat tcataaaatt
     1141 tttccgtagg atggcaatcg aacttaagta atagaaaatt tcagcgtctt gcttgttaat
     1201 ctctaaatac atttttaaat accgttcact tttctgtatt cgggctaaat cgaaattgac
     1261 gagagcggca gttttaagcg ccggcaagtt tttgggataa atttgaatgg cctcttcgag
     1321 caactcaacg ccctcttgcg agttgtttga ttcaatcaag cggttggcgc gcgcaacgcg
     1381 ttccagaaac cgtgaatact ctgggtttac ctcgatcatg agcattgaaa tgtcgtcgtt
     1441 ggggggattt ccttcggcga acttaagcac ttcatcgacg acaaaaggca ccaccgcttc
     1501 tgcaggtaac gaactcgctt tttgcaagag tgcatcaaac tgcgcgtttc caagctcttc
     1561 acccgcttta ttacggatct caataagacc atccgtgtag agaaaaagac ggtcgcctgc
     1621 ataaagacgc ccttcttttt cagcataact ttcattggct tcgtgcattg caccgacaaa
     1681 aagaccatcg gtgtcgagcg aatcgattgt gcttatggag gactcctctg tgtccgtggt
     1741 ttcttcagtg gaaaccgaac gcgcgcgcgc gattttcgcg tgttggtgcg acgcgttacc
     1801 aaaaagaaaa tggtgggtgt cgtcgatggt taaataaaac gcggttaaat aatcctgcgt
     1861 ggtaacaatt tctaaaagct ggttattgac ttctcggaat gtctgcaccg gcgaatgcgt
     1921 gttctgcgcc gcgcgtgcaa aagccacttt cgccattgtg gtgatgagcg cagcaggcac
     1981 accgtgaccc gacacatctg ccataagtac acccaagcgg ttaccgtgcg cggggaaaac
     2041 atcgaagtaa tcccctgaaa ctttttccat agggaggtag taactaaaga atttcaatcc
     2101 attccattct tcgattttag tcggtatgat tcctttttgg atttcagcgg caaagtcgag
     2161 ttccatttgg attagatggt ctttttgatg gagcgcttcg tttgcctccg tcagctctgc
     2221 ggtgcgcatc tccactttac gctccaaatt gtgttggtaa tcgcggatgg tttgcgtggc
     2281 ggtattgagc cccgtcgcca cattgataaa ctcggcgtct aacgcttcgg ggaaaagcat
     2341 ggtgtttttg ccctcgcgga tcccctgcgc ggtttgctcg atttcacgca acgcttgtaa
     2401 aatcaccgaa aaaagcataa acgccatcac cgctgcaatc gcgatggcaa aaagcgagaa
     2461 gcgcatcagc accgagaaac tcacgttgta ataaatcata aaattggcga caaaaagggt
     2521 cgcaatcatg atagatataa agaagagaac ttttgagcgc accgtcgaaa gagagatttc
     2581 tttgaattca at
//
LOCUS       DAOMPD010000386         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_15999_length_2589_cov_3.023283, whole genome shotgun sequence.
ACCESSION   DAOMPD010000386 DAOMPD010000000
VERSION     DAOMPD010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15999_length_2589_cov_3.023283"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLY93_11710"
     CDS             <1..730
                     /locus_tag="PLY93_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRP70190.1"
                     /translation="FLDDKNNDPLFFATVGALVKKKPESARAAILREIKEQDFTKAGN
                     RIPDLVHLLSVYKDISLESFLLEKLQSADTYADYRSGILKYLAEMKNWSENLKTHVAK
                     IFEDEAEPLTVRGSAAYALGKAGIDTAKPKLKAVLNKIEAMENGDEKKRYTRFRMQII
                     AGLILLKDDAVKEILFNMSRDDDEIVRLRAVRQIGALKIPDARPLLEYKSKFDPSVKI
                     QREAKKALSHLDKDTPDDLPSEDK"
     gene            742..1185
                     /locus_tag="PLY93_11715"
     CDS             742..1185
                     /locus_tag="PLY93_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70191.1"
                     /translation="MRRFARGVCIPFLFIFFPLHGYLSNDDVTLEKAFENKTANQLFL
                     KYQRTDGDYLVFYDLDGSILFLRYRVDKWDYANDNTRDALRRGITYHVFLKDFQRLAE
                     NELPKGVTGAGLSKAIQKRKIRRIREVYSGALVDIKPSALRDLRY"
     gene            1186..2421
                     /locus_tag="PLY93_11720"
     CDS             1186..2421
                     /locus_tag="PLY93_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRP70192.1"
                     /translation="MLFLLILILTLFSAFFSGSEAAIFSQDIRRLKRGTTDVFSPKLR
                     KTIIGWLKKPERVITGIILGNLVVNIAITDAGEAWLIELFADFNQRHVLFPLIITLYI
                     LTFSEVIPKIIALVFKDLWIRALQLPLRGWFRLSNRFTIPFDKIANAAVKSLKGVKSD
                     LTESELLEAVRFAERHGLLKGEEMHMLSRSIAFHHNTIYAAMIPRSQLLMLPAHSTLS
                     QCKKAFSQSTHSFATIYKKNPRDIQGVVYLRGVVQLALLKKKEIEEKMYSLDFLPASM
                     SLSRSLSFLIDARRDLAGVVDESGAFMGMVTLRGILNHILGSSFKTAPVDEYLTPIDA
                     RRYRVAAQMPLDRFNEIFRTTFDAALAETIGGFLLEKFDGFPHNDAEIEIGSLVFKDF
                     LVADHKITSFVLWVKKNAT"
BASE COUNT          715 a          598 c          560 g          716 t
ORIGIN      
        1 atttttagac gataagaata acgatccgct tttctttgca actgtcggcg cactggtgaa
       61 aaagaaaccc gaatctgcgc gcgcggcaat tttgcgcgaa atcaaagaac aagattttac
      121 aaaggcaggt aaccgaatac ccgacctcgt ccatttgctt tcggtttata aagacatctc
      181 gctcgaatcg tttcttttgg aaaaattaca aagcgccgac acatacgccg attaccgcag
      241 cggtattttg aaatacttgg ctgagatgaa aaattggtct gaaaatttga aaacacacgt
      301 tgctaaaatt tttgaagacg aagcagaacc tctaaccgta cgtggctcgg cggcgtacgc
      361 actcggcaaa gcgggtatcg acacggccaa acctaaactc aaagcagttt tgaataaaat
      421 tgaagcgatg gaaaacggtg atgaaaaaaa acgctatacc cgtttccgta tgcagattat
      481 tgcagggctc attttactca aagacgatgc ggtgaaagaa attcttttca atatgtcgcg
      541 cgacgacgat gagattgtac gtttgcgcgc ggtacgtcaa attggcgcgc ttaaaatccc
      601 agacgcgcgc ccacttttag agtataaatc caaatttgac ccgtcggtga aaattcaacg
      661 cgaagcaaaa aaagcgctgt cgcatctgga taaagacact cccgatgatt tacccagtga
      721 agacaaatga atacagcatt tgtgcgtaga tttgcgagag gggtatgtat cccttttctt
      781 tttatttttt ttcccctaca cggttatcta tccaacgacg acgttactct tgaaaaagca
      841 ttcgagaata aaacggcaaa ccaacttttt ttgaaatacc aaagaacaga cggcgattat
      901 ttagtctttt acgatttaga cggttcaatt ttatttttac gctaccgcgt cgacaaatgg
      961 gattacgcaa atgacaacac tcgcgatgcg ttgcgacgtg gcatcacgta ccatgttttc
     1021 ttaaaagatt ttcaacgcct tgctgagaat gaattaccca aaggcgttac gggcgcaggg
     1081 ctatcgaaag cgatacaaaa acgtaaaata cgccgtatcc gtgaagtata tagtggcgct
     1141 cttgtggata taaaaccgtc tgcgctgcgc gacttacgtt attaaatgct ttttttactt
     1201 attcttattt taactttatt ctctgctttt ttttcgggca gcgaagcggc cattttttcg
     1261 caggatatcc gacgcctcaa acggggcaca accgatgttt tttcgcctaa attacgaaaa
     1321 acgattatcg gttggctgaa aaaacccgag cgcgtcatca cggggatcat tttaggtaac
     1381 ctcgttgtga atattgccat caccgatgcg ggcgaagcgt ggttaatcga gctcttcgcc
     1441 gactttaacc agaggcatgt gctcttccct ctcattatca ctctctacat actcactttt
     1501 agcgaggtta tccctaaaat tatcgcgctt gtgttcaaag atttatggat tcgtgcgctg
     1561 caactccctt tacgcgggtg gtttcgcttg tcgaatcgct ttacaatacc gttcgataaa
     1621 attgccaacg ccgccgtaaa atctctcaaa ggcgttaaaa gcgacctcac cgaaagcgag
     1681 cttttagagg cggtgcgctt tgccgagcgt catggtttgc tcaaaggcga agagatgcac
     1741 atgctctccc gctctatcgc tttccaccat aacacgattt atgcagcgat gataccccgc
     1801 tctcaacttc tgatgttgcc cgcacattcg acgctttccc agtgcaaaaa agcatttagc
     1861 caaagcacac acagctttgc gacgatttac aaaaaaaatc cacgcgacat ccaaggcgtg
     1921 gtgtatttgc gtggcgtggt gcagttagcg ctcctcaaaa agaaagaaat tgaagaaaaa
     1981 atgtattcgt tagattttct acccgcgagt atgtcgcttt cgcgctcgct ttcgtttctc
     2041 atcgatgcgc ggcgcgacct tgcaggcgtt gtcgacgagt cgggcgcctt tatgggcatg
     2101 gtgaccttgc gaggaatatt gaatcacatt ttaggatcga gtttcaagac agcgcctgta
     2161 gacgaatatt taacgcccat cgacgcccgt cgctaccgtg tggcggcaca aatgcccctc
     2221 gatcggttca acgaaatttt tagaacgact tttgacgctg cgcttgcaga aacaatagga
     2281 ggatttctcc ttgaaaaatt cgatggtttt ccgcataacg atgcagaaat tgaaataggc
     2341 tctctggttt tcaaagattt tttagtcgcc gatcacaaaa ttacttcatt tgtattgtgg
     2401 gtgaaaaaaa atgctactta gcattgtcat attcctcttc tgcttagcag gcagcgcctt
     2461 tttttcggca actgaagtgg cgtttttttc tttgaatccg ctttctgtca ccaatggtaa
     2521 tcgccacaaa ctggaaaaac ttttccacag caaaaacgac atcatcgcga tgctccttac
     2581 gggtaataa
//
LOCUS       DAOMPD010000387         2587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16014_length_2587_cov_2.346761, whole genome shotgun sequence.
ACCESSION   DAOMPD010000387 DAOMPD010000000
VERSION     DAOMPD010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16014_length_2587_cov_2.346761"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..727
                     /locus_tag="PLY93_11725"
     CDS             23..727
                     /locus_tag="PLY93_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70193.1"
                     /translation="MDKPEQESQQGGQGAQTEPPKIEPKLDNDGIFKHAYALGQSAAE
                     KKTEWLKEVFGTADRAEIEKLVKKPKDNELPRTVLDQIAALQKENETLQASIKAQALN
                     DSISKEIGGSGFNLHNSEMVRTMLGNEYDFKQINGELVAHKKGSDQPVVIDNRFATIK
                     TILGTWAKEPSTSFLFAQQQQVTMPQGAHNGSTLKDGELTSRTFVAALKQSGQFSDAI
                     NGKPFDRERVNRLIRK"
     gene            963..1856
                     /locus_tag="PLY93_11730"
     CDS             963..1856
                     /locus_tag="PLY93_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70194.1"
                     /translation="MQSSTNMNDHILPKLAELAWGMLISQLKFVPRLNKWPNPLPGSN
                     ANEYRVPKISGGSAKDRLPGAPVIFEDRVAGYTPITAKQIYDAFVTDESYQSSTGFAY
                     FESLVESAVKNVVQEVETYAAEQVALQGGIGTVGTLGQAVTRANLQTARLAFVNNKIP
                     TGNLLAFLSGETMKDIADINEYTRFDAVGAVPRNALREGVADRVSGFELLESPYVYSP
                     NVGEHTNLLFDPSQIMYIFPAQAPISGDAQKAEFERDGIRLYLLKEYLAGYNGAYAYT
                     VSVNFGIKAVRPEGVIKLLGK"
     gene            1949..>2587
                     /locus_tag="PLY93_11735"
     CDS             1949..>2587
                     /locus_tag="PLY93_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70195.1"
                     /translation="MRIVDAMMQRIRMSTNKMMEQVTLNTYRNVRAGMGFSAEPDLAP
                     LRAMLSERQSYFDERLKKYRWLVKRFDNPSKKIGRRGHDVIREISLDTLEEGFLRGNP
                     TAFTSTELARKIVNKGTLFKSEEDALEALKGVQKDIINKIPLFNKDGELKFMLHTRAG
                     GIYSGKFRAMDVERYADMVAFTTAGEANTAANIAQAGEIGTRIVKWNSTGKGV"
BASE COUNT          761 a          640 c          663 g          523 t
ORIGIN      
        1 aaactaaaac agagaacgaa taatggacaa gccagaacaa gaaagccaac aaggcggcca
       61 aggcgcacaa accgagccgc ctaaaatcga gcccaagtta gacaacgacg ggatttttaa
      121 gcacgcgtat gcgttgggtc aatccgcagc ggagaaaaaa accgaatggc ttaaagaggt
      181 tttcgggaca gccgaccgcg ccgaaatcga aaaacttgtc aagaagccaa aagacaacga
      241 gctccccagg acggtactcg accaaatcgc cgcactgcaa aaagagaatg aaacactgca
      301 agcaagcatc aaagcacaag ctctaaacga ttccatctct aaagagattg gcgggagcgg
      361 gttcaatctg cacaattcgg aaatggtgcg cacaatgttg ggaaacgagt acgattttaa
      421 gcagattaac ggcgagttag tggcacacaa aaaaggctct gaccaacctg tggtgattga
      481 caatcgtttc gcgacaatca aaaccatttt gggcacatgg gctaaagagc catccacatc
      541 gttcttgttt gcgcaacaac aacaagtgac aatgccgcaa ggtgcgcaca atggcagcac
      601 acttaaagat ggcgagttga caagccgcac tttcgtggct gcgctgaaac agtcagggca
      661 attcagcgac gcgattaacg gcaagccgtt tgaccgcgag cgcgtaaata gactaatccg
      721 caaataattc cattgacgga aaaacttacg taattagata gagtcatcat ggcggttctg
      781 cccaccgcct caacaaaagg cgtcgggtgg ttccccgcaa ccaacacgaa accgagttcg
      841 tgtaaagcat ctcggactgg gtggcccccc gcagccaaat ggttctgcct accatcacaa
      901 aaggcgtcgg gtgactcccc gcagtcggaa accaattagg ccgaaaggcc ataggagact
      961 atatgcaaag ttcaacgaat atgaatgacc atattctacc caaacttgcc gaactggcat
     1021 ggggtatgtt aatatcacaa ttaaaatttg ttccacggct caataagtgg ccgaacccac
     1081 taccagggtc aaatgcgaac gaataccgcg taccgaaaat ctcgggcggg tctgcgaaag
     1141 accgcttgcc gggcgcaccg gtaatttttg aagaccgtgt ggcgggttac acacccatca
     1201 ccgcgaaaca aatttacgat gcgtttgtga ccgacgaaag ttatcaaagc tctaccggtt
     1261 ttgcgtactt tgaatcactc gttgaatcgg cggttaaaaa cgtcgttcaa gaagttgaaa
     1321 cgtacgcagc cgaacaagtc gcactacaag gcggcattgg tacagtcggc acgttagggc
     1381 aagcggtgac acgcgcaaac ctgcaaaccg cacgtttagc gtttgtgaac aacaaaatcc
     1441 ccacgggtaa cttgttggcg tttttgtcgg gtgaaaccat gaaagacatc gccgacatta
     1501 acgagtacac acgcttcgac gcagtgggcg cagtgccgcg caacgcgtta cgcgaaggtg
     1561 tggcagaccg cgtaagtgga ttcgagcttc ttgaatcgcc ttacgtgtat tcgcccaacg
     1621 tcggcgagca caccaacctg cttttcgacc cgtcgcaaat catgtacatt ttcccagcgc
     1681 aagcaccgat tagcggtgac gcgcaaaaag cggaatttga gcgtgatggc attcggctct
     1741 atctgctcaa agaatacctc gcagggtata acggcgcata cgcgtatacc gtctcggtaa
     1801 actttggcat taaggccgtc cgccccgagg gcgtgattaa gctcttaggt aagtagtgct
     1861 gaggtactat cgtgcagcgt tcaagtatgg cattttcgga atcttgcgag aagtcttacg
     1921 cgcgttgcac gatggtcaaa acgtcgaaat gcgaatcgtg gacgcgatga tgcaacggat
     1981 acgaatgagt acaaacaaga tgatggagca agtgacactt aacacttacc gtaatgtgcg
     2041 tgcaggcatg ggtttctcag ccgaacccga ccttgcgccg ttacgtgcaa tgttaagcga
     2101 gcggcaaagt tatttcgatg aacgactcaa gaagtatcgt tggcttgtga agaggtttga
     2161 caacccttca aagaaaatag gtcgtcgcgg ccacgatgtc attcgtgaaa tttcgttgga
     2221 taccctcgaa gagggttttc ttcgtggcaa tccaacggcg tttacatcga ccgagttggc
     2281 tcgaaaaatc gtcaacaaag gcacgctctt taagtccgaa gaggacgctc tcgaagcgct
     2341 gaaaggcgta caaaaagata taatcaataa aattcccctc tttaacaaag atggtgaact
     2401 taaattcatg ctacacaccc gcgcgggcgg aatatatagc ggcaaatttc gcgctatgga
     2461 tgtagagcgg tacgccgaca tggtagcatt caccacagcg ggcgaagcga acaccgccgc
     2521 gaatatcgca caggccggtg agataggcac gcgcattgtg aaatggaact ccaccggcaa
     2581 aggcgtg
//
LOCUS       DAOMPD010000388         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16072_length_2580_cov_3.729505, whole genome shotgun sequence.
ACCESSION   DAOMPD010000388 DAOMPD010000000
VERSION     DAOMPD010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16072_length_2580_cov_3.729505"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..666)
                     /locus_tag="PLY93_11740"
     CDS             complement(118..666)
                     /locus_tag="PLY93_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70196.1"
                     /translation="MKFKNSKKFITTCLMAVLALSIAFPLVAAKKKSKAKSKGSYKQI
                     TAVLPQFYKEYEKNALRATKKWKGKKVSANGTVGFLGGGMIIHTNSFENFGELSIDCN
                     VSENVMMKFSNGDTVDVFGEIDRIRFVLALGVTYIELENCQFKNIVSTEKDNTPSEED
                     VDKNSSEDESDDENENNNEDTP"
     gene            complement(663..1154)
                     /locus_tag="PLY93_11745"
     CDS             complement(663..1154)
                     /locus_tag="PLY93_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70197.1"
                     /translation="MMRSFTKYVLLLAIISFGYCGKKNQEIEKSKANKLGESLGNTMR
                     DLGAGLRDGISNDATAPIKLADELSTVLKLGAVKYNTRTNAITVYCEALTDIDLNVML
                     KIFDAQNQEMGRVKHRLKMESEDAQNIAFTFDATIDLSNARLIHLDRGKEKPKEKTKG
                     KSL"
     gene            complement(1151..1723)
                     /locus_tag="PLY93_11750"
     CDS             complement(1151..1723)
                     /locus_tag="PLY93_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70198.1"
                     /translation="MANKQSKTPKKRSSGNERQRKSSTKRVVHEVEIVPQAPPPPPSV
                     PNVPIVQVFTAPQGPQATHVPMQESISGAPFMLAIAGFIFGLPGILCLVACGTATSAV
                     ETSLGATVIILIIVFNVIPLAAGLIGGIISRNSPKNGGILMLVGAVFELLGVFFAQNL
                     FGWITAICYVIGGVIAMGQKKMVPIKTGNE"
     gene            complement(1824..2066)
                     /locus_tag="PLY93_11755"
     CDS             complement(1824..2066)
                     /locus_tag="PLY93_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70199.1"
                     /translation="MKIRSLSPDEHGCTAKVSEANGTPMEVMGVAFYKAAKKRCKKLK
                     VGQKVKLTPREIMYLFMKEVPQNGKRNIGMLAVFPY"
BASE COUNT          642 a          577 c          518 g          843 t
ORIGIN      
        1 attaaaaacc tcctctattt gtgcacgaaa attgatagtt ggcgcacagg ctcctacggg
       61 ataagggttc cttccagaac tcgctgcgcg agttcaggaa ggaacgatag ttatttttta
      121 gggagtgtct tcgttgttgt tttcattttc atcgtcgctc tcatcttcag atgaattttt
      181 gtctacatct tcttcggacg gtgtgttatc tttttctgtt gaaacaatgt ttttaaattg
      241 acaattttca agttcaatgt atgtaactcc aagagcaagt acaaaacgaa ttctatcaat
      301 ctcgccgaat acatcgaccg tatcaccatt ggaaaatttc atcataacgt tttcagaaac
      361 attgcagtca atacttaact cgccaaaatt ttcaaaagaa ttagtatgaa ttatcatgcc
      421 accaccaaga aaaccaaccg taccattcgc actaactttt ttgcctttcc attttttagt
      481 tgcgcgcagt gcattttttt catattcttt ataaaattgt ggtaatacgg ctgtaatttg
      541 cttgtaagag ccttttgact tcgctttact tttcttcttt gccgctacga gcggaaaagc
      601 aatgctaagg gcaagtactg ccattaagca agtagtgatg aattttttgc tgtttttgaa
      661 tttcatagag attttccttt tgtcttttct ttgggttttt ctttacctct atctaagtga
      721 ataagccgcg cattggagag atcgatggtt gcatcgaagg taaatgctat gttttgcgca
      781 tcttcgctct ccattttaag ccgatgcttg acgcgtccca tttcttggtt ttgtgcgtca
      841 aaaattttta acattacatt caaatcgatg tcggtgagtg cctcacagta aaccgtgatg
      901 gcatttgttc gtgtattgta ctttaccgca cccagcttta atactgtact taactcatcg
      961 gcaagtttaa tcggtgcggt cgcgtcattt gagataccgt cacgcaatcc agcgcctaaa
     1021 tcgcgcatcg tattacctaa actctcgccc aatttattgg ctttactctt ttcaatttct
     1081 tggttctttt tgccgcaata gccaaaacta atgatagcga gaagaaggac gtattttgta
     1141 aaagagcgca tcattcgtta cctgtcttaa tcggcaccat ctttttttgc cccatagcga
     1201 taacgccgcc aatcacataa caaattgcgg ttatccagcc aaagaggttt tgcgcaaaaa
     1261 agacgcccag aagttcaaaa acagcaccga cgagcataag tataccgccg tttttaggtg
     1321 aatttcggga tataataccg ccaatgagac cggcagcaag aggtataaca ttgaatacga
     1381 taatcaaaat tattaccgta gcacctaaac ttgtttcaac cgccgaggtt gctgtaccgc
     1441 acgcgactaa acagagaatg ccgggtaagc caaaaatgaa ccctgcaatg gccaacataa
     1501 agggcgcgcc gcttatagat tcttgcatcg gtacatgcgt tgcttgtggc ccttgtggtg
     1561 ccgtaaacac ttgcacgatg ggcacgttag gcacactggg tggtggcggc ggtgcttgtg
     1621 gtactatttc tacttcgtgt acaactcttt ttgtactact ttttcgttgc cgctcgtttc
     1681 cagagctgcg cttttttggg gttttagatt gtttatttgc catgataact actctatgta
     1741 gttaccatgc ttttttattg caaaaaaatt gaattttttt aatagttcat gcagaacccg
     1801 caaggcgggt tctgtatgaa cccttaatac ggaaacaccg ccagcatacc gatgttacgt
     1861 ttgccgtttt gtggtacttc tttcataaaa agatacatga tttctctcgg ggtcagtttg
     1921 actttttgac ccactttaag ttttttacaa cgcttcttcg ctgccttgta aaaagctaca
     1981 cccatcactt ccatcggcgt accgttcgct tcagaaacct tcgccgtgca accatgttcg
     2041 tcagggctga gagacctaat tttcacaaca aatctttgct ttttggcctg ctttttggca
     2101 atcttttcga tgtgaaaatt tgtcccgtgt tcgatttctt ttttggtgcc gctcttttgt
     2161 acaaactgaa tcaaccgctt ctctgttttg gttgcgcgtc ggtctataag gtcagtggga
     2221 gtatctacat gggtgctgct ttacgtgcgc tccgattttc tttcgggcac ttttctactt
     2281 gtataacact gttggcaaat catgtaacaa ccactgtttg cgctttcacc tcgcccctgg
     2341 caggcgcggt tgcatgattc tgtgtcggtt atgcgcccat aaaactgtga aaggccatcc
     2401 atcatatttc gaccttcatt gcaatcaaag cggacgctcc ctcgtgcgca tgcgtctgct
     2461 tgagattggc atgccatcat acaattataa tcgtaattgc aattctgagc acagatagat
     2521 gcagactctg cacaatgtga tgactgtgcc gccaaaagtg aaaggtttgc cgaagcaatt
//
LOCUS       DAOMPD010000389         2578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16086_length_2578_cov_2.771304, whole genome shotgun sequence.
ACCESSION   DAOMPD010000389 DAOMPD010000000
VERSION     DAOMPD010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16086_length_2578_cov_2.771304"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PLY93_11760"
     CDS             <1..1111
                     /locus_tag="PLY93_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70200.1"
                     /translation="IDLRQWDSPSAITIWQKLAQTNTADGKDILHRFMDYVDAERHGK
                     PSFSERATTPEKWVKGPLIAQKNENQVVGDCPVMSKKTVCCNLKTIDAVTNCVMGCNY
                     CAIQTMHSNDNVYTDADLAEKLRKVQLNPTRDYHFGTGQSSDSLLAGNKHGVFDALME
                     FCRENPNAFLEFKSKSSNTAYFEKANNFPPNLFFSWSLNPQEFIDAEEAHTASLEKRL
                     EAAERMVMHGFSVAFHFHPIVWFEGWQEAYSHVVKQVLSRFSPHDVVYVSLGTMTIPR
                     SVRKKIRAAGITTQVARNLTAENPEGKLTYPDAIKNELFAHVYREFDAWHADVFFYLC
                     MEEAKFWHPLFGFAYETNEDFEREMLKAIRLKLKW"
     gene            1116..1904
                     /locus_tag="PLY93_11765"
     CDS             1116..1904
                     /locus_tag="PLY93_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRP70201.1"
                     /translation="MPLKFVYRTLLGLLIVAFPLSFIFNTTLFRFLNCDKHGTFFLDT
                     FFLTLTSLADGFWVVLLILFFQSVLKNSSTRPLLILIFALILGNALLHSAKYFFDEPR
                     PLRVLGESVCVLGQRLTVRSFPSGHSFSVALFFMHLRPKRSFALAFFLFCISFLAILS
                     RAYVGAHFPRDMATGAAVGVLVYLTAEWIYKKINPIPLSPYKAKLSLAFAGIVVSLGY
                     IFLYEEKTTELTFALTPLAYGFVAYWIFYLTKIIFDYKKNTAKT"
     gene            complement(2012..>2578)
                     /locus_tag="PLY93_11770"
     CDS             complement(2012..>2578)
                     /locus_tag="PLY93_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70202.1"
                     /translation="VYNGIPSTTNAAADFAIGQANLTTNTTTDADFTIQSDYLKAPTD
                     VLLHNNHLYISDTGYHRVLVFNTVPTIVDQKPNLIIGHSAANNILPNRGLPVAAEGLN
                     YPTNLTAVGNTLAVADRDNHRALLYNLPITANAEAAQKVLGQSTMNANMPGVGQGQFN
                     QPNGIFFDGGFMWVVDSLDHRVQVVAIP"
BASE COUNT          642 a          596 c          593 g          747 t
ORIGIN      
        1 gattgatttg cggcagtggg attctcccag tgcaataaca atttggcaaa aattggcgca
       61 gacaaatacc gctgatggta aggacatcct tcaccgcttc atggattacg tcgacgccga
      121 gcggcacggt aaaccgtctt tcagtgaacg tgcaaccaca ccggaaaaat gggtgaaagg
      181 cccgctcatt gcacagaaga atgaaaacca agtcgtgggc gattgtcccg tgatgtcgaa
      241 aaagacagtg tgttgtaatt tgaaaaccat cgacgcggtc accaactgcg tgatgggctg
      301 caattactgc gcaatccaaa caatgcactc gaacgacaac gtttacacgg atgcggactt
      361 agcggagaag ctgcgtaaag tccagctcaa ccccacgcgc gactaccatt tcggcaccgg
      421 tcaatcatcc gactcactct tggcaggcaa taagcacggc gtgttcgacg cgctcatgga
      481 gttttgccgc gagaatccca acgctttctt agagttcaaa agtaaatcgt ccaacacggc
      541 gtactttgaa aaggcaaata attttccgcc caatcttttt tttagttggt ctttaaaccc
      601 gcaagagttc atcgacgccg aagaggcgca caccgcttcg ctcgaaaaac gactagaagc
      661 tgccgagcgc atggtaatgc atggatttag cgtcgccttc cattttcatc caattgtttg
      721 gtttgaaggc tggcaagaag catactcgca cgtcgtcaaa caagtccttt cacgtttttc
      781 gccccacgac gttgtgtatg tgtcgctcgg cacgatgacc attccccgca gcgtgcgcaa
      841 aaaaattcgt gcggcaggca tcactaccca agtggcacgc aatctcactg cggaaaatcc
      901 cgaaggtaag cttacctacc ccgacgcgat taagaacgaa ctcttcgcgc acgtctatcg
      961 agagtttgac gcctggcatg ccgatgtatt tttttattta tgtatggaag aagccaagtt
     1021 ttggcatcca ctttttggtt ttgcgtacga aacgaacgaa gactttgagc gggaaatgct
     1081 caaggcaatc cgcttaaaat tgaaatggta aaattgtgcc gttaaaattt gtttatcgta
     1141 cacttttggg tttacttata gtagcattcc cccttagttt tatttttaac acaacgcttt
     1201 ttagattttt gaattgcgat aaacatggga ctttctttct ggacactttc tttctcactc
     1261 taacttcact cgccgatggt ttttgggtgg tgttactcat actctttttt cagagtgttc
     1321 tcaaaaattc ttcgacacgc cctcttctta ttttgatttt tgcgctcatt ctggggaatg
     1381 cgcttttgca ttcggctaaa tattttttcg acgagccgcg cccgttgcgt gtcttaggcg
     1441 agagcgtgtg cgtcttgggt caacgcctca cggtacgcag ctttccttca ggccattcgt
     1501 tttcggttgc actttttttt atgcatctgc gccctaaaag gtcgttcgct cttgcatttt
     1561 ttcttttttg tatttctttt ttggcaattt tatcgcgcgc atacgtgggt gcgcacttcc
     1621 cacgtgacat ggcgacgggg gcggcggtgg gtgttcttgt ctatcttacc gccgaatgga
     1681 tttacaagaa aattaatcct attcctcttt ctccatacaa ggcaaaatta tcccttgcat
     1741 tcgctggcat cgttgtgtcg ttgggatata ttttcctcta cgaagaaaaa acaacagagc
     1801 ttacttttgc gctcactccc ctcgcgtatg gttttgtagc gtactggata ttttacttaa
     1861 cgaaaattat ttttgactat aaaaaaaata ctgcaaaaac ataggcaggg gattttagct
     1921 aatctcatct ttagacgaaa aataaaattg aacaatggtg tagatagtat aaaggtattg
     1981 atggatatct tggatagtag ttcttgaaaa tctacgggat tgccaccact tgcacgcggt
     2041 ggtcaagact atcgaccacc cacataaaac cgccgtcaaa gaaaataccg ttgggttgat
     2101 tgaattgccc ctgccccact ccgggcatat ttgcgttcat agtgctctgc cccaacacct
     2161 tttgcgctgc ttctgcattt gcggtgatgg gtaagttgta tagaagcgct cggtggttgt
     2221 cgcgatcggc gacagcaagc gtgttgccca ccgcagttaa gttggtgggg tagtttaacc
     2281 cttctgcggc gacaggcaat cctcgattcg gaagaatatt gtttgctgcc gaatgcccaa
     2341 tgattaaatt aggtttttgg tcgacgatcg tcggcacagt gttgaaaacc aacacacggt
     2401 gatagcctgt gtcagaaata taaagatgat tattgtgcag caaaacatcg gtgggggctt
     2461 tgagataatc ggattgaata gtaaaatctg catccgtcgt cgtgtttgtt gttaaatttg
     2521 cctgcccgat agcaaaatcg gcagcggcat ttgttgttga cggtatgcca ttatacac
//
LOCUS       DAOMPD010000390         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16207_length_2563_cov_2.156699, whole genome shotgun sequence.
ACCESSION   DAOMPD010000390 DAOMPD010000000
VERSION     DAOMPD010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16207_length_2563_cov_2.156699"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /gene="asd"
                     /locus_tag="PLY93_11775"
     CDS             <1..550
                     /gene="asd"
                     /locus_tag="PLY93_11775"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543513.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HRP70203.1"
                     /translation="NPRFHDRFLGGYYMTIYLSPQDDHRIHSPVDGRILGYYYQPGKL
                     FPVNNLAVNSINQLFSKNERLITYIQSEPGLVAVVKVDATSVGKIRVTYDPNIATNRW
                     IRLAKEYLYDEEIQIHKGAELGRFEMGSTVMLLFEKDTVELLDFEERQKMLLGQPIAL
                     FSRKKSPDVAEEKQRRQKKARR"
     gene            555..1343
                     /gene="proC"
                     /locus_tag="PLY93_11780"
     CDS             555..1343
                     /gene="proC"
                     /locus_tag="PLY93_11780"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRP70204.1"
                     /translation="MTIGIIGLGKMGSAIAQALAGKFEVVAYEKNENAIKNLRPSLVK
                     KIRIVESLDILFSAAGPVIIAVKPTQVKEVVSAIRDHRLVISIAAGVTLQSIDEARKE
                     NGPTVRAMPNTPLVIKRGITAFTGNQFCTSADIETTKSFFESVGESLYLENEKLLHAV
                     TGLSGSGPAYVYLFLQTLEDSGVLLGLSRDDSRRLAMHTVLGSAELARQSQSAPQNLI
                     HDVTSPGGTTAVALKSLHARGFPNAVMEAVAIAARRSKELAGEK"
     gene            1355..2266
                     /locus_tag="PLY93_11785"
     CDS             1355..2266
                     /locus_tag="PLY93_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRP70205.1"
                     /translation="MPSKKQESILSEEEILTLLRSLKASKKNSISGDDIAAFDTHPRA
                     YAATDSLIENTHYKRTWLLPKGIAYKLFARNWSDFLCKGILPRYALLNLNLSRKSATL
                     SFLSPFLHELDHLLVRHKMILIGGDTAYSQTDVFTLTLLGSEGTFIPRCASSIQVGDC
                     VVQLGAVGASDWARKCLMRKITDRMRTLFARPHLFAKLPSRHNLKAALHQSDSVDKTL
                     HILAAQNRVTLAIDVDAIDVYPRVQKGSHAILSAAEDLALFGIATKTSSAFRVIGRVV
                     SSDSHGVVYRHRGKIVTYAGGFEHFKN"
     gene            2235..>2563
                     /locus_tag="PLY93_11790"
     CDS             2235..>2563
                     /locus_tag="PLY93_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRP70206.1"
                     /translation="MLVDLNTSKIDFRGPLVVLHRPQIPQNTGNIARLCVAIQSPLII
                     TGRAGFDFSERPVRRAGLDHWNHLAFSHFPRFKDFYKKFGEHRLVAVTKAATRSALTF
                     DYTPNGI"
BASE COUNT          645 a          625 c          632 g          661 t
ORIGIN      
        1 caacccgcgt tttcacgatc gttttttggg tggctattac atgacgatct atctctcgcc
       61 gcaggatgac caccgtattc attcccccgt ggatggccgt atcttggggt attattacca
      121 gccgggaaaa ttatttcctg tgaataattt ggcggttaac tcgatcaatc aacttttttc
      181 aaaaaacgaa cgcctcatca cgtatatcca aagcgagcct gggctcgttg ccgtggttaa
      241 agtggacgca acgtcggtgg ggaaaattcg agtgacgtac gaccctaaca tcgccaccaa
      301 ccgttggatc cgtttagcta aagaatatct ttacgatgaa gaaatccaaa tccacaaagg
      361 cgccgagttg ggtcgctttg aaatgggctc caccgtgatg ctcctttttg agaaagacac
      421 ggttgagcta ctcgattttg aagaaaggca gaaaatgctt ttaggacaac cgattgcact
      481 ttttagccga aaaaaatctc ccgatgttgc agaagaaaag cagcgaagac aaaagaaggc
      541 aaggcggtaa aaaaatgacg attggaataa ttggtttagg taaaatgggc tctgcgattg
      601 cacaagcgct tgcgggtaaa tttgaggttg tggcgtatga aaagaatgaa aacgctataa
      661 aaaatttacg tccatctctc gtcaaaaaaa ttcgtatcgt tgagtcgttg gatattctct
      721 tctctgcagc cggccctgtc attattgcag tcaaacccac tcaagtgaaa gaagtggttt
      781 ctgcgatacg cgaccaccgc cttgtgatta gcatcgctgc aggagtgacg ctacaaagca
      841 tcgacgaagc gcgtaaagaa aatggcccaa cggtgcgtgc gatgccgaat acgcctctcg
      901 ttatcaagcg tggcattacc gcatttactg gaaaccaatt ctgcaccagt gcagatattg
      961 aaaccacaaa gtcatttttt gaaagcgtgg gagaatcgct ctatctagaa aatgaaaagc
     1021 ttttgcacgc ggtgacaggg ctttcaggtt cagggcctgc gtacgtgtat ctttttttgc
     1081 aaacgcttga agactcgggt gtacttttgg gcctgtcgcg cgacgacagc cgccgtcttg
     1141 ccatgcacac cgtgttgggt tctgccgagc tggcgcggca atcgcaaagt gcgccacaaa
     1201 atttaatcca cgatgtcaca agcccgggcg gcaccacggc ggttgcgttg aaatcgctcc
     1261 acgcacgggg cttcccgaac gcagtgatgg aagcggtggc aatcgcagcg cggcgctcca
     1321 aagaacttgc gggtgagaaa taacgtacag caagttgccg tctaaaaaac aagagagtat
     1381 tttaagcgaa gaagaaattc ttacgctttt gcgttcatta aaagcatcta agaaaaatag
     1441 catctccggc gacgacatcg ccgcgtttga tacacatccg cgtgcgtacg ccgccaccga
     1501 cagtctcatc gaaaacacgc attacaagcg tacttggctt ttacccaaag gtattgcgta
     1561 taaacttttt gcgcgcaact ggtctgattt tttgtgcaaa ggaattttgc cacgttatgc
     1621 gcttttgaat ttaaatttga gccgcaaaag cgccactctt tctttcctct ctcctttttt
     1681 gcacgaactt gatcatctgc ttgtgcggca taaaatgatt ctcattggtg gcgatacggc
     1741 atactctcag acagatgtct ttacgctcac actgttgggt tctgagggta cgttcatccc
     1801 tcggtgtgca agtagcatcc aagtgggtga ctgcgtcgtc caactggggg cggtgggggc
     1861 gagcgattgg gcgcgtaagt gcctcatgcg aaaaatcacc gatcgtatgc gtactctttt
     1921 tgccaggccg catctcttcg caaagctacc ttcgaggcac aacttaaaag cggcgctcca
     1981 tcaatccgac agcgtggata aaacgttgca tattttggca gcacaaaatc gggtgacact
     2041 cgctattgat gtcgacgcca tcgatgtcta ccctcgtgtg caaaaaggtt ctcacgcaat
     2101 tttgagtgcc gctgaggatt tggcgctttt cggcattgca acgaaaacct cttctgcttt
     2161 tcgcgttatc gggcgtgtcg tttcgtccga tagccacggc gtcgtgtatc ggcatcgggg
     2221 taaaattgtt acctatgctg gtggatttga acacttcaaa aattgatttt cgcgggccgc
     2281 tcgtcgtact ccaccggcca caaattccgc aaaacaccgg taacattgcg cggctttgtg
     2341 tcgcgatcca aagcccgctt attatcaccg gccgcgcggg gtttgatttt agcgagcgtc
     2401 cggtgcgtcg cgcggggcta gaccattgga atcatttagc gttctcccat tttccgcgct
     2461 tcaaagactt ttataaaaaa tttggagaac accgccttgt cgcagtgacc aaagctgcca
     2521 cccgctctgc gttgacgttt gattacactc ccaacggtat ttt
//
LOCUS       DAOMPD010000391         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16298_length_2552_cov_3.154586, whole genome shotgun sequence.
ACCESSION   DAOMPD010000391 DAOMPD010000000
VERSION     DAOMPD010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16298_length_2552_cov_3.154586"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLY93_11795"
     CDS             <1..360
                     /locus_tag="PLY93_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRP70207.1"
                     /translation="NPYKKLRDTYPRGTVIEGVVSGVVDFGVFIKFDEGYEGLAHIST
                     MEKEQATNFKKTIQKGQPIKAVVKSIDPENRKISLSLKDVNYALEKIEMRQYLEKESS
                     SKAETTSPFANLKTLVS"
     gene            357..1088
                     /locus_tag="PLY93_11800"
     CDS             357..1088
                     /locus_tag="PLY93_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRP70208.1"
                     /translation="MKRNALTVVGQICQRELKSYFQSALAYFILSITFSLAFLWGYAY
                     IKHETTSSLAIQRIFYMLSGTTMITGVLISMRLIAEEKMQGTIELLFTSPLTEGQLVL
                     GKFFSALSFLAIQAFLTLPIPLLVVFYGGANVGHVVAGFSGVLLIGAATAAMGLFYST
                     LTKTQLLAAVMTAANLVFFLLLGFFSPFIDQPLKSVLREMSFYVHFMDFEKGVVSLKH
                     VTFYLSIVIFYLYIATLALSIRRWR"
     gene            1136..>2552
                     /locus_tag="PLY93_11805"
     CDS             1136..>2552
                     /locus_tag="PLY93_11805"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRP70209.1"
                     /translation="MNLLRQRVSGCIGLSLIFFYFAVNLSAKGFLSATLFFIAALIYA
                     ACGVVLYTLYKGEERKGGFFRILQFAYWLILLAAVCYFMQTDAIWTSLTDNKTNAIAE
                     RLRQILQILYFVGFALVVLCLLIASILRSEAKEAMNAVWTLGVLLLFLVAANYWTHIR
                     PAQVDMTLLRKFSLSQDSRNLLRGVEKDVAITAFYPFFSDLYRNVELTLRDAASVNSK
                     ISYTFIDPLREKERADAFKVDRIGTILVASGTNKTKFEIVDEDALRRLERELVSAILQ
                     VSGKPLNIYYSQGHDEKPLSGAFKDDTLEAFDENLRALKHHVRQLTPKEGFPQKMPAA
                     DLVFILGPRRDFSAQEKKTILDYFEKGGKLFIAIDPESSADFSFLLTPLNVKYVKEKV
                     LSDYALPPGKTTLQTINYSDHAITAPFVRLSEERKITIFPGAGYLEDLAKPNTDYDVN
                     YFLLSHYSSWIDKIRNGLRDDK"
BASE COUNT          663 a          573 c          579 g          737 t
ORIGIN      
        1 aatccttaca aaaaattacg cgacacctat ccacgcggta ccgttatcga aggcgtggtt
       61 tccggcgtcg ttgattttgg tgtgttcatc aaattcgacg agggttacga aggtctagcg
      121 catatttcca cgatggaaaa agaacaagcg acaaatttca agaagacaat ccaaaaagga
      181 cagccgatta aagcggtggt caaaagtatc gatccagaaa atcgtaaaat ttcgttgtcg
      241 ctcaaagatg tcaactatgc gttagaaaaa attgaaatgc ggcaatactt agaaaaagaa
      301 agttcaagca aggctgagac caccagtccc tttgcgaact taaaaactct agtgtcgtga
      361 aacggaatgc tttgactgtc gttgggcaaa tttgccagcg tgagctcaaa agttattttc
      421 aatcggcgct cgcttatttt attttaagca ttacgttcag tttagcattc ctttggggct
      481 acgcctacat caagcacgaa accacctcgt ccttagcgat acaacggatt ttctatatgc
      541 tttcgggcac gacgatgatt actggcgttt taatttcgat gcgccttatc gccgaagaaa
      601 aaatgcaagg cacgattgaa ctactcttca catccccgct taccgaaggc cagttggtgt
      661 tgggaaaatt tttttctgcg ctctctttcc tcgccatcca agcattctta acgctgccca
      721 tcccactttt ggtggtattt tatgggggag ccaacgtcgg ccacgtcgtt gcgggcttca
      781 gcggcgtttt gctcatcggt gcggcgacgg cagcgatggg acttttttac tcgactctca
      841 ccaaaacgca acttttagcg gcggtaatga cggcggcaaa tcttgttttc tttctacttt
      901 taggtttttt ctcgccgttc atcgaccaac cgctcaagag cgtgttgcgc gagatgagtt
      961 tttatgtcca tttcatggat tttgaaaaag gagtggtgtc gctcaaacat gtcacttttt
     1021 atttaagtat tgttattttt tatttataca ttgcaacgct cgcgttgagt atacggcggt
     1081 ggagatagtc catgaacaat agatgccgag gtggtgtatg aatttggaac ggtgggtgaa
     1141 tttattacgt caacgggtaa gcggatgcat tggtttgtcg ctcatttttt tttattttgc
     1201 agttaatctt tccgctaaag gatttttatc cgcgacactt ttttttattg ctgcactcat
     1261 ttacgccgct tgcggtgtgg tgttatacac cctttacaaa ggcgaggaac gtaaaggggg
     1321 attttttcgc atcctccaat ttgcatactg gctgatttta ttggctgcag tttgttattt
     1381 tatgcaaacc gacgcgatat ggacaagttt aacagataat aaaacaaatg caatcgctga
     1441 acgcttacgg cagatattac aaatattata ttttgtcggc tttgctctcg ttgtgctttg
     1501 tctcctgatt gcgtcgattt tgcgatcgga agccaaagaa gcgatgaacg ctgtatggac
     1561 attaggggtg ttactgttat ttctggttgc cgcaaactat tggacacaca tccgtcctgc
     1621 gcaagtggac atgacactct tgcgtaagtt ttcgctttcg caagatagcc gtaatttatt
     1681 gcgcggtgtt gaaaaggatg tcgccatcac ggcgttctat cccttcttta gcgatttgta
     1741 ccggaatgtg gaactcactt tgcgcgacgc ggcgtcggtg aattcaaaaa ttagttacac
     1801 cttcatcgac cccttgcgcg aaaaagagcg cgccgacgca ttcaaggtcg accgtatcgg
     1861 caccatcctt gtcgctagcg gcacaaacaa aacaaaattt gaaattgtcg atgaagatgc
     1921 gctgcgtcgc ttggagcgcg aattggtgtc ggcgatttta caggtgagcg gcaaaccact
     1981 caacatttat tattcacagg ggcatgacga gaaaccgctc tcaggggcat tcaaagacga
     2041 cacactggaa gcattcgacg agaatttacg cgcgctcaaa caccatgtac gacagcttac
     2101 ccccaaagag ggctttcccc agaaaatgcc cgccgccgac ttagtgttca ttcttggccc
     2161 ccgccgtgat ttttcggcgc aggagaaaaa aacgatcctc gattattttg aaaaaggtgg
     2221 caaacttttt atcgctattg atcctgaatc ttccgccgat ttttcttttc ttttaacgcc
     2281 gctgaacgtc aaatatgtca aagaaaaagt tttgagcgat tacgcgctcc ccccaggaaa
     2341 aaccacgcta caaacaatta actattcaga ccacgccatc actgcgcctt ttgtgcgttt
     2401 atccgaagag cgtaaaatta cgattttccc cggcgcgggt tatttggaag accttgcaaa
     2461 accgaatacc gattacgacg tgaattattt tttattgagc cactacagca gttggattga
     2521 taagatccgc aacggtttgc gcgacgataa aa
//
LOCUS       DAOMPD010000392         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16320_length_2550_cov_2.429659, whole genome shotgun sequence.
ACCESSION   DAOMPD010000392 DAOMPD010000000
VERSION     DAOMPD010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16320_length_2550_cov_2.429659"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLY93_11810"
     CDS             <1..320
                     /locus_tag="PLY93_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70210.1"
                     /translation="TEIKMLEHASDIINRNGMLSMVPQGGKSLVTQLQTMPPDMVATQ
                     GNMIIFTNGAMSANEADFMLEAGDMMFGGNAGDMTEFFNFFDSFFDELYNMGFTKIDF
                     EKL"
     gene            348..>1553
                     /locus_tag="PLY93_11815"
     CDS             348..>1553
                     /locus_tag="PLY93_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRP70211.1"
                     /translation="MKKLKTLFTTLGITAFVFALPAQDINKIEQGELVPLGKLRPLSV
                     SEMPLNKHGINAGVSTSNSGYFDSSLNQMLFLGFYRPYATYLMDENHLFTLRGKWAIT
                     EFTRDIKGSNNQVVKTRADASSSVGSLEVASAELHFNRLNITAGRAFYKLGRGLLFGN
                     FADGAEVSQSFRWFEYKVLAVYSGEYGQSACGLSVSGCGGRSPYDIVPGRSFDAQLVN
                     AGKRVFGGGEITSAPYWGSRLSAQFLYTKDFNTDANKDGKKFTYDAFYAGLAARGFIG
                     APSLRYMLEYAYQGGSTINDGAINQSAAIGAHALQMEVAYNPPFYQEKFKPMVIAQYA
                     FASGDSDRNDGTNAAQVNTKGNDTGFYHFGIFSGGLALKPRLQNIHVARVGIAGRPLG
                     NFYALRNLML"
     assembly_gap    1554..1563
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1564..1766
                     /locus_tag="PLY93_11820"
     CDS             <1564..1766
                     /locus_tag="PLY93_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70212.1"
                     /translation="EGKISDGDASEKSADIGQGFDATLVYDFRSDLKFYYGFGLFQRG
                     NAYKANKRDLIQVHLLSVTINF"
     gene            1795..>2550
                     /locus_tag="PLY93_11825"
     CDS             1795..>2550
                     /locus_tag="PLY93_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70213.1"
                     /translation="MKRYIKTTITVCLAFMAAHCAQSKPSVDIKVNPSVQLQDGSPLS
                     LPNYDELSPQLVQTSDGKVALFFVSNRPADGYTCTNSYTVFIATTTVAYTGGTLPPFN
                     SPQVVYDGNTNCLSSSATPFKIAAYAVSSNYQLIIDNGTAIISYVITGTAWDQNTPTT
                     FAGSTHRLLGVDAYSLYHEIFTLNDGGQIYYTKISESPTTSIAFQGMMGIPVESIAPV
                     PSDVETNGFFFLDQDIGMGVKDSAMALPHLSLSL"
BASE COUNT          679 a          595 c          602 g          664 t
ORIGIN      
        1 gcaccgaaat aaaaatgctc gaacacgctt cggacattat caaccgcaac ggtatgttgt
       61 cgatggtgcc acaaggcggt aaatcgctgg tgactcaact gcaaacaatg ccacccgaca
      121 tggttgcaac acaggggaac atgattattt ttactaacgg cgcaatgtcc gccaacgaag
      181 cagattttat gctggaagcg ggtgacatga tgtttggggg taatgccggt gacatgaccg
      241 aattttttaa tttttttgac agtttttttg atgaactcta caatatggga tttactaaaa
      301 ttgactttga gaaactgtaa aactaaacaa atcccctgaa gtattttatg aaaaaactga
      361 aaacgctctt tacaacgctt ggtataactg cctttgtttt tgctctccct gcacaagata
      421 taaacaaaat cgaacaaggc gaactggtgc cactgggaaa gttacgtcca ctctcggtga
      481 gtgaaatgcc gctcaacaag cacggtatca atgcgggggt gtcgacatcg aactcagggt
      541 attttgatag ctcgctcaac caaatgttgt tcttggggtt ttatcgcccg tatgccacgt
      601 atttgatgga tgaaaaccat cttttcacgc ttcgcggtaa atgggcaatc accgaattta
      661 ccagagatat aaaaggctcg aacaatcaag tggtaaaaac ccgcgccgat gcaagctcgt
      721 cggtgggctc acttgaggtc gcgagcgccg aattacattt caaccgactc aacatcactg
      781 ctggccgtgc gttttacaaa ttggggcgtg gtttactttt cggcaacttt gccgatggcg
      841 ccgaggtgtc gcaatcattc cggtggtttg aatacaaagt acttgcggtg tattcgggcg
      901 aatatggcca aagcgcatgc ggcttgagcg tgtctggttg cggtggccgc agcccctatg
      961 acattgttcc tggccgctcg ttcgatgcgc agcttgtgaa cgcaggtaag cgtgtttttg
     1021 gcggagggga gattacctct gcaccgtatt ggggcagccg cctctcggca caatttttat
     1081 ataccaaaga ctttaatacc gatgctaaca aagacggtaa aaaatttacg tacgatgcct
     1141 tttatgccgg ccttgcagcg cgcggtttta tcggcgcacc gtcgttgcgg tacatgttag
     1201 aatacgcata tcaaggcggt tcaaccataa acgatggcgc cataaaccaa agtgccgcca
     1261 tcggcgcgca cgcattgcaa atggaggtgg cgtataaccc cccattctac caagaaaaat
     1321 ttaagccgat ggtaatagcg cagtacgcgt ttgcctcggg tgattctgac cgcaacgatg
     1381 gtaccaacgc cgcacaagtc aacaccaagg gcaacgacac agggttttac cattttggta
     1441 tcttttctgg cggtcttgca ttgaagccac gcttgcaaaa catccacgtt gcgcgtgttg
     1501 gtattgccgg tcgcccgctg ggtaattttt atgcgctgag aaatttgatg cttnnnnnnn
     1561 nnnccgaagg aaaaatttcc gatggcgacg cttccgaaaa aagcgcagac atcggccaag
     1621 gctttgacgc cacgctcgtc tatgatttcc gttccgattt aaaattttac tatggctttg
     1681 gtcttttcca gcgtggcaac gcgtacaaag cgaataaacg cgatttaata caagtacatt
     1741 tattgagtgt gaccattaac ttttgagata ctttgagaaa tttatggagg atttatgaaa
     1801 cggtatataa aaactacaat caccgtctgt ttagcattca tggcggcgca ctgcgcacaa
     1861 tcaaagccca gcgtggacat taaggtgaac cccagcgtcc agctccaaga cggctcaccg
     1921 ctgtcgctgc cgaattacga tgaactgagc ccacagctgg tgcaaacgag cgacggtaaa
     1981 gtggcgctct tttttgtatc gaataggcca gccgatgggt acacttgtac aaatagttac
     2041 acggttttta ttgcaacaac gactgtggca tacacaggtg gaacacttcc gccttttaat
     2101 tcaccacaag tagtttatga tgggaatacc aattgcctaa gctcaagcgc tactccattc
     2161 aaaattgcgg cgtatgctgt gagtagcaat taccagctta ttatcgataa tggcactgca
     2221 attatatctt atgtaattac gggtactgcc tgggatcaga acacaccaac aacattcgca
     2281 ggctcaacac accgtctctt gggtgtcgat gcgtatagtc tttatcacga aattttcaca
     2341 ctgaatgacg gtggccaaat ttattataca aaaatatctg agtcaccgac aacgtctatc
     2401 gcctttcaag gcatgatggg aatccccgtg gaatctatcg cgccggtacc cagcgatgtg
     2461 gaaaccaatg gcttcttttt cctcgaccaa gatattggaa tgggggtaaa agacagtgca
     2521 atggccttgc cgcacctatc gctttctctt
//
LOCUS       DAOMPD010000393         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16348_length_2546_cov_2.782015, whole genome shotgun sequence.
ACCESSION   DAOMPD010000393 DAOMPD010000000
VERSION     DAOMPD010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16348_length_2546_cov_2.782015"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLY93_11830"
     CDS             complement(<1..420)
                     /locus_tag="PLY93_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70214.1"
                     /translation="MENPPVAENPKEQKAPHPKKQETPAHEKQNLNKEKKKDHTALIE
                     RIRDVLNYGNSLQVRDTLNTLTRLTADEQKSLLPELKKTCESKDPLVLRKIAEFIGVA
                     PFNDLDNELVKFLDDKNNDPLFFATVGALVKKKPESAR"
     gene            complement(473..763)
                     /gene="hisE"
                     /locus_tag="PLY93_11835"
     CDS             complement(473..763)
                     /gene="hisE"
                     /locus_tag="PLY93_11835"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRP70215.1"
                     /translation="MNWEFLVELEGLLKDRFNKRPQGSYTTKLFEAGSQKILQKVGEE
                     SIEYILDAQNADKEKAVYEGADLLYHFMVSLVAQGWSLGDIVQELEKRHQKH"
     gene            complement(760..1239)
                     /gene="mlaD"
                     /locus_tag="PLY93_11840"
     CDS             complement(760..1239)
                     /gene="mlaD"
                     /locus_tag="PLY93_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRP70216.1"
                     /translation="MKKMEFYTGLFICFGLFCFAYIALSLGGLSLWRDNRYTLYANFV
                     SISGLKPGAVVEIAGVGVGNATRIELNHNKARLTLRLNKGIQIPADSAVAIRTRGLIG
                     EKFVKITPGADDTILKPDEEILETESVVDLEELIGKFMFDSKEESNSENVPTKDKKR"
     gene            complement(1241..2026)
                     /locus_tag="PLY93_11845"
     CDS             complement(1241..2026)
                     /locus_tag="PLY93_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP70217.1"
                     /translation="MKNAIELKGIFKSLSGQLVHENLSLEVERGKTTIVVGASGAGKS
                     VMLKYILGFLKPDQGDILVEGSSILNLRESEMKKVRSRYGVVFQGAALFDAMTVFENV
                     ALPLVEKTKKTSAEIEAQVMHSLTEMKLQDARHKMPAELSGGMQRRVALARALQLNPE
                     IVLFDEPTTGLDPETSENIYDLFIETQRQLNYTALIVSHDIPRIFRAGDKIAVLNERK
                     IESVVKPKDLKKAHGWLKRMLTPRSPESVSKKSIISESRKGKV"
     gene            complement(2029..>2546)
                     /locus_tag="PLY93_11850"
     CDS             complement(2029..>2546)
                     /locus_tag="PLY93_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP70218.1"
                     /translation="LLAELAPVLTALLIVGRAGSALCAELGVMRLSEQIEALECLAID
                     VYNFLVSPKLLAALIAVPLLIFIFTVSGILGGYVSGSLILGVSVGDYYQSIRDVMDAE
                     TIRMALIKGIVFSIFIITVTAYKGLYVHQSANNITGKGALALSRATTQAVVIASVGIL
                     ILDYLLTAVLI"
BASE COUNT          639 a          617 c          558 g          732 t
ORIGIN      
        1 gcgcgccgat tcgggtttct ttttcacaag tgcgccgaca gttgcaaaga aaagcggatc
       61 gttattctta tcgtctaaaa atttcactaa ctcgttatct aaatcgttaa aaggcgctac
      121 cccgataaac tcggcaattt tacgcaacac gagcggatct ttactttcgc aagttttctt
      181 aagttcgggc agcaaggatt tttgttcatc ggcggtaagg cgtgtgagcg tgtttaaggt
      241 gtcgcgcact tggagcgagt tgccataatt caacacatcg cggatgcgtt caattaaagc
      301 ggtatggtcc tttttctttt ctttgtttag attttgtttt tcgtgggcag gtgtttcttg
      361 cttcttggga tggggcgctt tttgctcttt gggattttca gcaacagggg gattttccac
      421 atgtttaggt aaaggtgccg catataaaga aagaacgctg agacagtaaa tactaatgtt
      481 tttgatgacg tttttccagc tcttgaacga tatcccccag cgaccagccc tgcgccacga
      541 gagaaaccat gaaatgatag agtaaatctg caccttcata taccgctttt tctttatcgg
      601 cattttgagc gtctaaaata tactcaatcg actcttcgcc gaccttttgg agtatttttt
      661 gtgaccctgc ctcaaaaagt tttgtcgtgt aagacccctg ggggcgttta ttaaacctgt
      721 ctttaagtag accttctaac tctacaagaa attcccaatt catcgttttt tatcctttgt
      781 gggaacattc tcgctgttac tttcttcttt actgtcgaac ataaatttac cgatcaattc
      841 ttctaaatcg accaccgact cggtctctaa aatttcttca tccggtttaa gaatcgtatc
      901 gtccgcgccc ggggtaattt tcacaaattt ttcaccgata agtccgcgcg tgcggatagc
      961 caccgccgag tcggcgggta tttggatgcc tttattcaag cgcaacgtta aacgcgcttt
     1021 attatgattg agctcgatgc gggttgcatt ccccacgccc acgcctgcaa tctcaacgac
     1081 cgcgccgggt ttaagccccg agatggagac aaagtttgca taaagcgtgt aacgattatc
     1141 ccgccacagg cttaatcctc ctaacgaaag tgcgatatac gcaaagcaaa aaagaccaaa
     1201 gcagataaaa agtcctgtgt aaaattccat ttttttcatg ttatactttt cctttacggc
     1261 tctcgctgat aatacttttc tttgacacac tctctgggga gcgcggcgta agcatacgct
     1321 ttaaccaacc gtgcgccttt ttcaaatctt tgggtttaac gacactttcg attttacgtt
     1381 cgtttaagac ggcaatttta tctcctgcac ggaaaatgcg cggtatatcg tggcttacaa
     1441 taagcgcggt gtaatttaac tgccgttgag tttcgataaa taaatcgtaa atgttttcgc
     1501 tggtttcggg atctaatccg gtggtgggtt cgtcgaaaag tacaatttcg ggattgagtt
     1561 gcaacgcgcg tgcgagcgcc acacgccgct gcatgccgcc cgaaagctcg gcgggcattt
     1621 tatgccgcgc atcttgcaat ttcatttctg tgagcgagtg catcacttgc gcttcgattt
     1681 ctgcggatgt tttttttgtt ttttccacga gggggagcgc tacattttca aagacggtca
     1741 ttgcatcaaa gagcgccgcg ccttggaaca ccacgccata gcgagagcgc acttttttca
     1801 tctcgctctc acgtaaattt aaaatgctcg agccttcgac aaggatatcg ccttgatcgg
     1861 gtttaaggaa acctaaaata tacttgagca taacactttt acccgcaccg cttgcgccaa
     1921 cgacaatcgt ggttttcccg cgctcgactt cgagcgataa attttcgtgt acaagctggc
     1981 cgctcaagct cttaaagatg ccttttagtt cgattgcgtt tttcatggtt agattaaaac
     2041 tgccgttaaa agatagtcta aaatgaggat acccaccgaa gcaatcacca ccgcttgcgt
     2101 cgttgctcgc gaaagcgcta acgctccttt accggttatg ttgttcgcgc tttggtgtac
     2161 gtacaaacct ttatacgcag tgacagtgat aataaaaata gaaaagacaa tccctttaat
     2221 gagcgccata cggatggtct ccgcgtccat gacatcgcgt atgctttggt aataatcccc
     2281 caccgagacg cctaagatta gcgaacccga cacatagccg cctaaaattc cgcttacggt
     2341 aaagataaaa attaacagcg gcaccgcgat gagcgcagcg agcaatttag gcgagactaa
     2401 aaaattatac acgtcgatgg ccaagcactc caacgcttcg atttgctctg aaaggcgcat
     2461 cacacccaac tcggcgcaca gagcgctgcc tgcccgcccc acgattaaaa gtgcggttaa
     2521 aaccggcgcc aactcggcta agaggc
//
LOCUS       DAOMPD010000394         2543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16384_length_2543_cov_3.025723, whole genome shotgun sequence.
ACCESSION   DAOMPD010000394 DAOMPD010000000
VERSION     DAOMPD010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16384_length_2543_cov_3.025723"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLY93_11855"
     CDS             complement(<1..350)
                     /locus_tag="PLY93_11855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP70219.1"
                     /translation="MTTNIELSVILPTYNEAENIPILIPLLQKVLAKYNYEILVMDDN
                     SPDGTYRIVEKMVRQDNRIRCVRRMANRGLSQAVVEGFSVAQGDAMLVMDADMQHDEA
                     AIPQFIEHFRAGAD"
     gene            487..798
                     /locus_tag="PLY93_11860"
     CDS             487..798
                     /locus_tag="PLY93_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRP70220.1"
                     /translation="MIIPDFPDIEVFDWDFANVHKLLKRHKVSTIECEQVFFNEPFLI
                     YEDTEHSIQELRYYSLGVTNEYRHLTVIFTIRNRKIRPLSARPMTKNEVKNYENIKKG
                     T"
     gene            773..1057
                     /locus_tag="PLY93_11865"
     CDS             773..1057
                     /locus_tag="PLY93_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRP70221.1"
                     /translation="MKTLKKVPKFKSEVEESEFWSKHDLTDYFDLSKAKRVKFPNLKP
                     STKLISIRLPEGLIEDIKILANRNAVPYQSLIKLYLADSVKREKNWQTRL"
     gene            complement(1154..2047)
                     /locus_tag="PLY93_11870"
     CDS             complement(1154..2047)
                     /locus_tag="PLY93_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRP70222.1"
                     /translation="MAKIVGFTFIRNAIEYDYPIRESILSLLPLVDKVVVAVGKSKDD
                     TLRLVRGIDKNKIHIIETVWDDSKREGGRVLAEETQKAYNEVIADKWAFYIQGDEVLH
                     EADYPRIREALVFYEDNPHVDGLLFKYRHFYGSYDYVGSSRRWYRREIRVVRPPRFAL
                     APQLKIYSYKDAQGFRKNENQKLRVKEIDAHVHHYGWVKPPEAQMKKQQTFHKLWHDD
                     SWMQQNIPKAAEFDYSGIDALEHFTGTHPKVMQERIREKNWRFDFDISRSTYSVKEKI
                     SRWVERYTGYRPGEYKNYVKV"
     gene            complement(2107..>2543)
                     /locus_tag="PLY93_11875"
     CDS             complement(2107..>2543)
                     /locus_tag="PLY93_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase U32"
                     /protein_id="HRP70223.1"
                     /translation="KALDDIALHKPFDRQLIGELDKVASRKYFSGFLTRGREETLPED
                     ERDFQNYDGNTRYQSHYYAGCIKRQDADGVAWFELKTRVEEGDALEVFFPGQFDSRWF
                     TAGQLKRGDTMHSVLSGGIGEVGMYIPFAVPEHAFLARQIKH"
BASE COUNT          729 a          603 c          501 g          710 t
ORIGIN      
        1 aaatctgccc ccgcgcgaaa gtgctcaatg aattgcggta tcgctgcttc atcgtgctgc
       61 atatccgcgt ccatcaccaa catcgcgtca ccctgtgcaa cggagaagcc ctcgaccaca
      121 gcttgcgaaa ggccacggtt tgccatgcgc cgcacgcaac ggatacgatt gtcttgtcgt
      181 accatttttt caacaatgcg gtatgtgcca tcaggcgaat tgtcatccat caccaaaatt
      241 tcataattat actttgccaa caccttttgt aaaaggggga ttaaaattgg tatattttca
      301 gcttcgttgt aagtggggag aatgacggag agttcaatgt tcgtagtcac agacccgtac
      361 agctataatt caaagctaca gctacaatca aaaaagcgag ttataggctc gccggcttag
      421 agtcctacta cgtcatttac tcgaaaaact tgacagattg tatttctgta tatattatgt
      481 atatacgtga ttattcctga ttttcctgac attgaagtct tcgattggga ttttgcaaac
      541 gtgcataaat tgctcaaaag acataaggtt tcgactatcg aatgcgagca agttttcttt
      601 aacgaaccgt ttctgatata tgaagatacc gaacattcaa ttcaagagct tcgatattac
      661 tccttaggtg ttaccaatga gtatcggcat cttacagtaa tatttactat ccgtaaccga
      721 aagattcgtc ctctttcggc tcgtccaatg actaaaaatg aggtaaaaaa ctatgaaaac
      781 attaaaaaag gtacctaagt ttaaatctga agttgaagaa tctgaatttt ggtcaaaaca
      841 cgatttaacg gattactttg acttatcaaa agctaagcgc gttaaatttc ctaatcttaa
      901 gccaagtaca aagctaattt ccattagatt acccgagggg cttattgaag acattaaaat
      961 tctcgctaat cgtaatgctg tcccttatca atcactaata aaactttatt tagctgactc
     1021 tgtaaaaaga gaaaagaatt ggcaaacccg actttgattt atctttaatc aaaaaataga
     1081 tgcccaatat gagaaaacac aaggaaaacg accggtcaaa cgataaattc attcaaattt
     1141 ctaaacgccc ccgctacacc ttcacataat tcttatactc gcccggacga taccccgtgt
     1201 aacgctcaac ccagcgtgaa attttttctt ttaccgaata cgtcgaacgg ctaatgtcaa
     1261 aatcgaaccg ccagtttttc tcgcgtatgc gctcttgcat caccttgggg tgtgtacccg
     1321 tgaaatgttc taacgcgtcg atacccgaat aatcaaactc tgccgccttg ggtatattct
     1381 gctgcatcca tgagtcgtcg tgccacagtt tatgaaatgt ctgttgtttt ttcatttgcg
     1441 cttcgggcgg ttttacccaa ccgtaatgat gtacgtgcgc gtcgatttct ttaacgcgca
     1501 atttttgatt ctcgttttta cgaaaccctt gcgcgtcttt atacgaatat attttgagct
     1561 gcggcgcgag cgcaaagcgc ggcggcctta caactcgaat ttcacggcga taccaccgac
     1621 gcgacgaacc gacgtaatcg tacgaaccgt aaaaatgcct gtacttaaaa agaagcccat
     1681 cgacgtgggg attatcttca taaaagacta acgcttcacg aatacgagga taatccgcct
     1741 catgaagaac ttcatcaccc tgaatataaa aagcccactt atcggctata acttcattgt
     1801 acgctttctg cgtttcttcg gccaagactc gaccgccttc gcgctttgag tcgtcccaca
     1861 cggtttcaat tatatggatt ttgttcttat cgataccgcg cacgaggcgt aatgtatcgt
     1921 ctttactttt acccacggcc accacaactt tatcgacgag aggcaagagc gataaaatcg
     1981 attcgcgtat gggataatcg tattcaatcg cgttgcgaat aaaagtaaag ccgacgatct
     2041 tagccacaat tccgtacagc tataattcaa agctacagct acaatcaaaa taaaaaaata
     2101 taccctctaa tgttttattt gtcgtgccag aaacgcatgt tctggcacgg caaacgggat
     2161 atacatgcct acctcgccaa taccgccgct taaaaccgag tgcatcgtat caccgcgttt
     2221 tagttggcca gcggtaaacc agcgcgaatc aaattgtcct ggaaaaaaaa cttcgagtgc
     2281 gtcgccttct tcaacgcgcg tcttgagctc aaaccacgca acgccatcgg cgtcctggcg
     2341 ttttatacac cccgcgtagt aatgcgattg atagcgcgtg ttgccgtcgt aattttgaaa
     2401 atcgcgctca tcttcgggga gtgtttcttc gcgaccacgc gttaaaaaac ccgagaaata
     2461 tttacgcgac gccactttgt caagttcgcc gatgagctgc cggtcgaatg gtttgtgtaa
     2521 ggcaatatcg tcgagagctt tgc
//
LOCUS       DAOMPD010000395         2533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16456_length_2533_cov_2.581517, whole genome shotgun sequence.
ACCESSION   DAOMPD010000395 DAOMPD010000000
VERSION     DAOMPD010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16456_length_2533_cov_2.581517"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1023
                     /locus_tag="PLY93_11880"
     CDS             37..>1023
                     /locus_tag="PLY93_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP70224.1"
                     /translation="MLANGASAEVREKLCGSVSSGGTESILLAMKTYRDMARQERGVK
                     HPEMVLPTTAHVAFHKAAQYFNIKIRFVNFDASYRAKIDEVKRAINHNTVVVVGSAPT
                     FPHGVIDPIEEMSELARKKGVPFHTDACLGGFILPWAKKLGYNVPKFGFELPGVTSIS
                     VDTHKYGYAAKGTSVILYRNEKIRHHQFFTATNWPGGMYFSPTFAGSRPGGLSAACWA
                     ALVSIGEKGYTDAAKKILAAADYIKKEIHKMPDLKIMGDPLYVIAFSAAAGIDIYKVM
                     EQMTHKGYSLNGLHRPACVHIALTLRHTEPGVKERFIEDLKAAVAYVKAHPSE"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1187..1981
                     /locus_tag="PLY93_11885"
     CDS             1187..1981
                     /locus_tag="PLY93_11885"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRP70225.1"
                     /translation="MPKGYPGRYPPPPHIDLAVRSQAIRELKGPDPETLPQPLKKRIF
                     TAIGARLMKWYIELVYRTSKQIAFEIHPDTEALLSNPRGQFIIALWHNRLMYTIYSLA
                     DNVAGRGHDILAIISQSSDGELIARVTKLWGAFTARGSSSRGGTAALKKILRYTKLHF
                     HPLITPDGPRGPVYEVKEGLPAMAHLTNLPIVPMCYDVEKKWIARSWDQFIIPKPFTK
                     AVLCYGAPIYLDKNMPIEEACIHIQKIMLEQVVYTENLLKRKNQKA"
     gene            2074..>2533
                     /locus_tag="PLY93_11890"
     CDS             2074..>2533
                     /locus_tag="PLY93_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70226.1"
                     /translation="MKVEGSTFLVTGGSSGLGLAASEELFMRGANIVIADLLHAELAH
                     FQGKSHHTNNRIVYSKTSVTEPESIQDTVGLAFREFGVLHGVINCAGIGAIVKTVAKK
                     KFGALENARRVIEINLLGTYNVVSIAASAMSAQAANADGERGVFINTASVA"
BASE COUNT          659 a          623 c          645 g          596 t
ORIGIN      
        1 aaatttgaag cggaaattgt tgcaatgacg gcaaatatgc tagccaacgg tgcaagcgcg
       61 gaggtgcgcg aaaaactgtg cggttcggtc tcgtcgggtg gcaccgagag catcctcttg
      121 gcgatgaaaa cctaccgcga catggcacgc caagagcgcg gagtaaagca ccccgaaatg
      181 gtgctcccca ccacggcgca tgtcgcattc cacaaggcag cgcaatactt taatatcaaa
      241 attcggtttg tgaattttga cgcatcatac cgtgcaaaaa tcgacgaggt caaacgcgcc
      301 atcaatcaca acactgttgt cgttgtgggt tcggcaccga cattcccgca tggcgttatc
      361 gatcccattg aggaaatgtc ggagttggcg cgcaaaaagg gagtaccgtt ccataccgac
      421 gcctgcttgg gtggttttat tttaccgtgg gcaaaaaaac tgggatacaa cgtgcccaag
      481 ttcggttttg aattgcccgg agtcacctct atatcggtgg acacgcacaa atatggttat
      541 gcggcgaaag gcacctcggt gattttatac cgcaacgaaa aaatccgcca ccaccaattt
      601 ttcactgcga ctaactggcc gggcggcatg tatttttctc ccacgtttgc cggttcgcgt
      661 ccgggggggt tgtcggcggc gtgttgggct gcgctcgtct ctatcggcga gaagggttat
      721 accgacgctg caaaaaaaat cttggctgcc gctgattata taaaaaaaga aatacataaa
      781 atgcctgact taaaaatcat gggcgatcca ctctatgtga ttgccttttc agccgcagcg
      841 ggaatcgaca tctataaagt gatggaacag atgacgcaca aggggtattc tttgaatgga
      901 ctgcaccggc ctgcttgtgt gcatattgcg ctgactctta gacacaccga gcctggagtg
      961 aaagagcggt ttattgaaga cttgaaagcg gctgtcgcgt atgtaaaagc gcacccttcc
     1021 gagnnnnnnn nnntctgcgc cgatttacgg catggcggcg acactaccgg tgcgcagcgt
     1081 ggtgggcgat ttactcaaaa cgtatatgga cgcatactat aaggtgtaag ataaaaaaga
     1141 cacctgttat tcaatagtaa tttggtctat gccgaaacgc tataaagtgc ctaaaggcta
     1201 tcctggacgc tatccaccgc caccgcacat cgacttggcg gtgcgcagcc aagcgatacg
     1261 cgagctcaaa ggccccgatc ccgaaacact cccacagcct ctcaagaagc gcatctttac
     1321 tgcaattgga gcgcggctga tgaagtggta tattgaattg gtgtaccgca cctctaaaca
     1381 aatcgcgttt gaaatccacc ccgacaccga ggcgcttctc tcaaaccctc gcggtcagtt
     1441 catcattgcg ctttggcaca accgcctcat gtataccatt tacagtttag ctgacaatgt
     1501 cgcagggcgt gggcacgaca ttttagcgat tatttcgcaa tcgtcggatg gagagctaat
     1561 cgcgcgcgtg acaaaactgt ggggagcgtt caccgcacgc ggctcttctt cgcgcggtgg
     1621 tacagcggcg cttaagaaaa ttttacgcta cacaaaatta catttccatc cactgattac
     1681 ccccgacggg ccacgcggac cagtatacga agtgaaagaa ggcctgcctg cgatggcgca
     1741 cctcaccaac ttacccattg tgccgatgtg ttatgatgtc gaaaaaaaat ggatcgcccg
     1801 ctcgtgggat caatttatta tccccaaacc ttttaccaag gcggtgctct gctacggcgc
     1861 tcccatttat ttggacaaaa atatgcctat cgaagaagca tgcattcaca ttcaaaaaat
     1921 tatgctggaa caagtcgttt atacagagaa tttgttgaag agaaaaaatc aaaaagcata
     1981 gcgcttagat tcaagctact attgctttaa ttaaaactca ttgaaataaa ctttaagatt
     2041 ggcgcaccga ctctttctct gggagcatcg tgcatgaaag ttgaagggag tactttttta
     2101 gtgactggcg gcagttcggg gttggggctt gcggccagtg aagagttatt catgcgcggt
     2161 gcgaatatcg tcatcgccga ccttctccat gccgagctgg cgcattttca gggtaaatcc
     2221 caccatacaa ataaccgcat cgtttacagc aaaaccagcg tcaccgagcc cgaaagtatc
     2281 caagacacgg ttggtttagc gttccgcgaa tttggcgtac tgcacggtgt tattaactgt
     2341 gcaggcatcg gcgcgattgt aaagacagtg gcgaagaaaa aatttggtgc gcttgaaaat
     2401 gcgcgacgcg tcattgagat taaccttttg ggtacgtaca atgtggtgag tattgccgct
     2461 tcggcgatga gcgcccaagc cgccaacgcc gacggcgaac gcggtgtgtt catcaatact
     2521 gcgtcggtgg cgg
//
LOCUS       DAOMPD010000396         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16525_length_2526_cov_2.983408, whole genome shotgun sequence.
ACCESSION   DAOMPD010000396 DAOMPD010000000
VERSION     DAOMPD010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16525_length_2526_cov_2.983408"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLY93_11895"
     CDS             complement(<1..996)
                     /locus_tag="PLY93_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70227.1"
                     /translation="MSTVHRVKKYRFHYVAFLLSLSTHILHAAPIGWGDAILVEPRAE
                     EYSVCCHEGEGQLYAVVSRLKSRFVADQPTLVDIVLNYTQNGRFVSRVVSTVMLAPSG
                     ELFPLHPSVSALGNEILIAWQETSTATCAANNAEDSCAWGSAAGIYYVYSSSGPEGFG
                     GKQALPATVGKLNAILPMAKMTAQGRHLIFYQEPTSGTRFMLSAATGGRGVFSGITPV
                     AQISAGVRGALFPSALRRGNRIDIVYQNRAAATLIDDIFRGFSIDNGVTWSSNLRITE
                     NGQQNFSGKLTNTKDKLVFVWQSNPKKVWSIFAAPEGQEAIQVSDGEAPSYLPTIV"
     gene            complement(1240..2196)
                     /locus_tag="PLY93_11900"
     CDS             complement(1240..2196)
                     /locus_tag="PLY93_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP70228.1"
                     /translation="MFIRFWGVRGSLATAITARQVEEKIRKVLELASPADLLSKEAIA
                     NFIQNLPLSLRGTYGGNTTCIEIQTSENKTVVIDAGTGLRALGNALMTRGKLTGKDDA
                     AFFFTHSHWDHIQGLMFFVPIFIPGNTFHFYSCFPDIETRLRYQMVTTHFPLTFDELG
                     SNKVFHSFNESENVEVYDLQVSAKAVRHPGGCYSYKFKQPNGKTFIFCSDAEFNLEIL
                     EDIGPFIDYFFGADVLVFDTQYTFEESLQKIDWGHSSAAIATDIAIKSEVKKLVLYHH
                     DPSYDDAKMDSVLLQAIKYKSMMAPNHPLEIVAAYEGLEIEL"
     gene            2309..>2526
                     /locus_tag="PLY93_11905"
     CDS             2309..>2526
                     /locus_tag="PLY93_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRP70229.1"
                     /translation="MNMHAVILAAGRGKRMQSDLPKVLHVLGGKPLLWHVLDQVKAAG
                     FSSATVVVSYKKEMVQESVETWMKTNSG"
BASE COUNT          650 a          656 c          563 g          657 t
ORIGIN      
        1 aacaatcgtc ggcaagtaac tcggcgcttc cccatcgctc acttgaatcg cttcttgtcc
       61 ttcgggtgca gcaaaaatag accaaacttt tttagggttg gattgccaca cgaacaccaa
      121 tttatctttt gtattcgtta gctttcccga aaaattctgc tgcccatttt cggtaatccg
      181 caaattgctc gaccacgtta ccccattgtc aatactaaag ccacgaaaaa tatcgtcgat
      241 gagcgtcgct gcggcacggt tttggtacac aatgtcaatc cgattacccc gcctcaacgc
      301 gcttgggaaa agcgctccgc gtacaccagc cgatatttgc gccacagggg taatcccact
      361 aaaaacacca cgcccccccg tcgccgccga cagcatgaaa cgagttcccg atgtcggctc
      421 ttgataaaaa attaaatgtc tcccttgtgc cgtcatttta gccataggta aaatggcgtt
      481 gagcttaccc accgtcgcag ggagtgcctg tttcccccca aaaccttccg gcccgctact
      541 gctatacacg taatagatac ccgcagcgct cccccacgcg cacgaatctt ccgcattgtt
      601 cgccgcgcac gtcgctgtgg atgtctcttg ccaagcaata agaatttcat tccccaacgc
      661 tgagaccgaa ggatgcaacg ggaataactc gcccgacggt gcgagcatta ccgtgctcac
      721 tacacggcta acaaagcgcc cattttgggt atagttcaaa acaatatcga caagtgtcgg
      781 ttgatcggcg acaaaacgtg atttaaggcg cgaaaccacc gcatacaatt gcccctcgcc
      841 ttcatggcag cacacgctat actcttccgc ccgtggttca acaaggattg catcccccca
      901 accaataggc gctgcgtgca gtatatgcgt cgaaagagag agaagaaacg caacgtaatg
      961 aaaacgatat tttttgactc gatgaacagt agacatttac gaatggcggt agcgttgagc
     1021 gtatttgtcg acaagagcaa ttatcatttt ctgataagtc actctatatt tttgactgca
     1081 cttcggggcg tttctgcagc gagttctttt tcttatattt tgcatatctt tctatccagt
     1141 atccggcgcc gaaaattctt atctatacac agcccccatt aattgaggtc gcttctatcc
     1201 cctcacccat acaattctcc cgccaccgca tcattttttt cacaactcaa tctccagccc
     1261 ctcgtacgcg gccacaattt ctaacggatg attcggcgcc atcatgctct tatacttaat
     1321 cgcctgcaag agtacgctgt ccatctttgc atcatcgtac gaggggtcgt ggtggtaaag
     1381 caccaatttt ttcacttcgc ttttaatggc gatgtcagtg gcaatcgcgg cggaggagtg
     1441 cccccaatcg attttctgca aactttcctc gaaagtatat tgagtatcga aaacgaggac
     1501 atccgcaccg aagaaataat cgatgaacgg gccgatgtct tcaagtattt caagattgaa
     1561 ttcggcgtca gagcaaaaaa taaacgtttt accattgggc tgtttaaatt tgtacgagta
     1621 acaacccccg ggatgccgga ctgctttcgc ggaaacttga aggtcgtaca cttcgacgtt
     1681 ttcactctcg ttgaaggaat ggaagacttt attggaaccc aattcatcaa aggtgagcgg
     1741 gaagtgcgtc gtcaccattt ggtagcgcaa gcgcgtttcg atgtcgggaa aacacgaata
     1801 aaaatggaaa gtattgccgg ggataaagat gggaacaaaa aacataagac cttgtatgtg
     1861 gtcccagtgc gagtgcgtaa agaaaaatgc cgcgtcgtct ttgccggtga gtttgccacg
     1921 cgtcatgagc gcattgccga gcgctctgag cccagtgcct gcgtcgatga caacggtctt
     1981 attttcactg gtttggattt caatgcaagt ggtgttgccg ccgtaggttc ctcttagcga
     2041 aagcggcaaa ttttgtataa agtttgctat agcttcttta gaaagtaaat ccgccgggct
     2101 tgccaattcc agcactttac ggattttctc ttcaacttga cgggcggtga tagcggttgc
     2161 gagcgagccc cgaacacccc aaaaacgtat aaacatcttc cttaatttaa tcggcgtatg
     2221 ctcctacaat gattttatct ccagtttgac atccattccc ttctaaacac tttacaccct
     2281 gtctttcaat gccattttag ccgcatcgat gaacatgcac gccgttattt tggcggcagg
     2341 ccgcgggaaa cgaatgcaaa gcgacttgcc aaaggttctt catgttttgg gcggaaagcc
     2401 gctcttgtgg catgtcctcg atcaagttaa agctgccgga ttttcctcgg cgacggtggt
     2461 tgtctcttac aaaaaagaaa tggtgcaaga atcggtggaa acgtggatga aaaccaatag
     2521 cggcat
//
LOCUS       DAOMPD010000397         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16654_length_2511_cov_1.987785, whole genome shotgun sequence.
ACCESSION   DAOMPD010000397 DAOMPD010000000
VERSION     DAOMPD010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16654_length_2511_cov_1.987785"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLY93_11910"
     CDS             <1..341
                     /locus_tag="PLY93_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70230.1"
                     /translation="RLKAAKDTKPVEFVTFDNWENFSQLRGELATDDLLVVVLTRKGT
                     FSDAPVMYKIPDIVQKQFKAHGVLLVFPRAIQPSGMAWGQDEEMITPIRDAWRSSWRS
                     MRRMFRRSKK"
     gene            complement(353..1633)
                     /locus_tag="PLY93_11915"
     CDS             complement(353..1633)
                     /locus_tag="PLY93_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRP70231.1"
                     /translation="MPILLFLILGVFHSLFADTAPTEALPLYLYYLFALIGGLLLNFM
                     PCVLPVLSLKAMALVDKGTKDTSSTKKKSIAQNIHRALPYTLGVVVTFSVVGGLFLIL
                     RASGQAIGWGFQLQNPIFVAVIALLFAAMAFNLFGVFEIRAPVITTRPAIGEAMTGVI
                     AVVAATPCTAPFMASALGASLTLPAFTGFLMFPLLGVGMALPFLIFALFPSSGKMLPK
                     PGAWLFTFKGVLGFMMVASSLWLVWVFDALTGSAFTLLTAILFVVFALYIYGRNQMAP
                     SKRALRFVRYAVIFLSLGFAFHFAFAGFTPIAKKTTVAVAWQNFSVSARDASLAKGEP
                     VFIDFTADWCITCKVNEEVAFTKKVMQHFVDKGVVLLRADFTNLDDTIADEITKLGRA
                     GLPVYAFYNRAGKVTLLPQVLTEGLLIKAIDGNT"
     gene            1796..>2511
                     /locus_tag="PLY93_11920"
     CDS             1796..>2511
                     /locus_tag="PLY93_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRP70232.1"
                     /translation="MATSHTRETLKHSSIFSLFTSLSRIFGLARDVLKAYAFGTGPLS
                     VAFDIAFRLNNMLRNLVAEGALSQSFVPLYTKYAAEGEEKAREAAGVVVVFTALLMGC
                     ITVLTIFLLPYFLPLVLADDKIPTEEMLLTVRLSQLLFPYLVMMSVASLYMGIQYAHK
                     IFWAASFGPALLNIVILVVFGAYAVYIRATHATFGVRAIYIFSWATLLAAAVQVVFQI
                     YTVHRRGLSPRYSFRFKHGAL"
BASE COUNT          649 a          627 c          635 g          600 t
ORIGIN      
        1 ggcggctcaa agcggctaaa gatacaaaac cggttgagtt tgttactttc gacaattggg
       61 aaaatttttc gcagctacgc ggcgagttag ccaccgacga tcttctcgtc gtcgtactca
      121 cgcgcaaagg cactttctcc gatgctccgg tgatgtataa aattcccgac atcgtgcaaa
      181 agcaattcaa agcgcacggc gtgttgcttg tttttccgcg tgcaattcaa cccagtggca
      241 tggcttgggg tcaagacgaa gagatgatta cccccatccg cgacgcatgg cgttcgagct
      301 ggcgcagtat gcggcggatg tttcggcgga gtaaaaaata aaagcggtat tgttacgtat
      361 ttccgtcgat ggcttttatt agcaagcctt cggtcaagac ttgcggcaac agcgttactt
      421 tacccgctct gttgtaaaaa gcatacaccg gcaatcccgc acggcctaac ttcgtgattt
      481 cgtccgcgat ggtgtcgtcg aggttcgtga agtcggcgcg taacaacaca acgcctttat
      541 cgacaaagtg ttgcatcact tttttagtga acgctacttc ttcgttgact ttgcaagtga
      601 tgcaccagtc ggcggtaaag tcgataaaca ccggctcgcc cttggcgagc gaagcgtcgc
      661 gcgcgctcac cgagaagttc tgccatgcca ccgccacggt tgtttttttg gctatcggtg
      721 taaatcccgc aaaggcaaaa tggaacgcaa accccaacga caaaaatatg acggcgtaac
      781 gcacgaaacg aagcgctcgc tttgacggcg ccatttggtt gcggccataa atgtagagag
      841 caaatacaac aaaaagaatt gcggtgagta aagtgaacgc agaaccggtg agcgcgtcga
      901 aaacccaaac gagccacagc gacgacgcca ccatcataaa gcccagcacg cctttgaaag
      961 taaaaagcca cgcgccgggc ttgggcagca ttttgccgct tgaaggaaaa agcgcaaaaa
     1021 taagaaatgg caacgccatg ccgacaccta aaagagggaa catgagaaaa cctgtaaacg
     1081 cgggcagcgt gagcgacgcc cccaacgcgc tcgccataaa gggagcggtg catggtgttg
     1141 ctgcaacaac agcaataacg ccggtcattg cttcaccgat ggcggggcgt gtagtgatta
     1201 ccggtgcgcg gatttcaaac accccaaaaa gattgaatgc cattgcagca aaaagaagcg
     1261 caatcaccgc gacaaaaata ggattctgga gttggaatcc ccaaccgatg gcttggccgc
     1321 ttgcacgcaa aattaaaaag agaccgccca ccacgctaaa ggtaacgacg acccccaacg
     1381 tgtaaggcaa ggcgcgatgg atgttttgcg ctatagattt ttttttagtt gaacttgtgt
     1441 cttttgtgcc tttgtcaaca agcgccatcg ctttaagcga gagaaccggc aacacgcacg
     1501 gcataaagtt taagagtaga ccaccgatga gtgcaaaaag ataatagagg taaagcggaa
     1561 gcgcttctgt cggtgcggtg tcggcaaaaa gactgtggaa aactcccaaa attaaaaaaa
     1621 gtagaatcgg catgttaagg ggaatataat accgaaaaga cgatacgcca actgtaaaat
     1681 acgcatccta aagtagtgag gtttctatgg tgagtcaaca aaaatcatat agtttttgaa
     1741 tctcgcgaag ctcagcaacc gctttaccgt gtatttttga accacactcg ggtatgtggc
     1801 cacatcgcac acccgcgaaa ctcttaagca ctcgtcgatt ttttctctct tcacttcgct
     1861 ctcgcgcatc tttggtctcg cccgcgatgt cttgaaggca tacgcgtttg gtactggccc
     1921 actttcggta gcgtttgata ttgctttccg actgaacaac atgctgcgca accttgtcgc
     1981 cgagggggcg ctctctcagt cgtttgttcc gctctacacc aaatacgccg ccgagggtga
     2041 agaaaaagca cgcgaagcgg caggcgtggt ggtggttttt accgccctat tgatgggatg
     2101 tatcacggta ctgactatct ttttgctccc atattttttg cccttggttt tagcggatga
     2161 caaaatcccc acagaggaaa tgctgcttac cgtacgcttg agtcagctcc tttttccgta
     2221 tttagtgatg atgagcgtgg cgtcgctcta catgggtatc cagtacgcgc ataaaatttt
     2281 ttgggcagcg agctttggtc ctgcgctctt aaacattgtg atcttggtgg tctttggtgc
     2341 gtacgccgtg tatatccgcg ccacgcatgc tactttcgga gtacgtgcga tttacatttt
     2401 ttcgtgggcg acgcttttgg cggcggcagt gcaagttgtt tttcaaattt acaccgtcca
     2461 ccgccgtggc cttagcccac gttacagttt ccgctttaaa cacggcgcac t
//
LOCUS       DAOMPD010000398         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16661_length_2510_cov_2.390224, whole genome shotgun sequence.
ACCESSION   DAOMPD010000398 DAOMPD010000000
VERSION     DAOMPD010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16661_length_2510_cov_2.390224"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PLY93_11925"
     CDS             complement(<1..1408)
                     /locus_tag="PLY93_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRP70233.1"
                     /translation="MVFNRIKYVSFAVLAPLLMLNCFTSEKALPGVYGEGQIVGKVVG
                     TDGMAIQGVTVTTTYQGVTYTKTSSNKGNFTFKIDNVKRGEGFQVHLAKTEFQSTDYA
                     TVITLPNLKVDMGNLVMYIEGDEGSGITRQITGQILDNFSFKPLIGANVAVTDSAGQV
                     RTGVTGEDGKFTIQSNYFKGPARDADGNLLPPPYPSYQLSAFKSNYITRTDLIAKMSS
                     DDVPIEDGQIKLYNKFGSVYGYVVEDTITPETPLNGVEAKIVNSNNQTHICPTGGPYN
                     PEPLSETDFCPDMDDDFGTANYGANGGGFKIKSQFIFLGNRYNIELSKTSAACQARTT
                     GATGCYRSKTLFSDIYLTGNNAITGEKTNLLWDSWIEGTVTGGAGAGVVVKLYDVNNT
                     YLTQTTSTAGGKFIFDNPQILRAKVYKMTFEKDGYYARLLGVPDGTRPCGGGTMYQSE
                     VPITITANGSNDVGSVPMT"
     gene            complement(1398..>1953)
                     /locus_tag="PLY93_11930"
     CDS             complement(1398..>1953)
                     /locus_tag="PLY93_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70234.1"
                     /translation="NRIYGIVFDLAYQQNRSYVIDEQTTYIPAQGNVPSPITGYMRSD
                     YLIMHLMFSYRYSPARVLSKIKFMRPVAKYFKAIAGNLQIGGFLKTPLSAQLEIQDPN
                     VGDPNDPFYDTAKFTKPVSAGVMAGIGFEVRLGSAIFFFEGQYFRGLTDTFGKVESRL
                     FNTKEMAEQGIYASTGFKFGIYGF"
     assembly_gap    1954..1963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1964..2207)
                     /locus_tag="PLY93_11935"
     CDS             complement(<1964..2207)
                     /locus_tag="PLY93_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70235.1"
                     /translation="MQSTTAASDAEQDYTTSKGPESYKRTLYMGLLVGMNAMGPNEGV
                     DISTAGLTAAQKARVETAMENGNSWQISPVIGLRSEL"
BASE COUNT          623 a          613 c          568 g          696 t
ORIGIN      
        1 ccgtcatagg cactgagcct acgtcgtttg aaccatttgc tgtaattgtt attggcacct
       61 cagattggta cattgtgcca ccaccgcaag gtcttgtccc atcgggtacg cctaaaaggc
      121 gcgcatagta gccatctttt tcaaacgtca ttttatagac tttagcgcgt aaaatttgtg
      181 ggttatcgaa aataaactta ccacccgctg tgctggttgt ttgagttaag taggtgttgt
      241 tcacgtcgta cagtttaacc acaacccccg cacccgcacc accggtaacg gtgccttcga
      301 tccatgaatc ccagagaaga ttggtctttt caccggtgat ggcgttgttg cccgttaaat
      361 agatatcgct aaacagtgtt ttcgagcgat aacagcccgt ggcgccggtg gtgcgcgctt
      421 ggcaagcagc gctggtttta ctgagctcaa tgttgtaacg attccccaaa aaaataaatt
      481 gactttttat tttgaagccg cctccattcg cgccataatt agccgtacca aaatcatcgt
      541 ccatatcggg gcaaaaatcg gtttcggaca atggttcagg gttatacggg ccaccggtgg
      601 ggcatatatg cgtttgatta ttggaattga caatctttgc ttcaacgccg ttcaacggcg
      661 tttcgggtgt aatagtgtct tcaacgacgt acccgtacac cgaaccgaat ttattgtaga
      721 gtttgatttg tccatcttca atcggtacgt catccgacga cattttagcg atgagatcgg
      781 tgcgagtgat atagtttgat ttaaaggcac tcagttggta tgaaggataa ggggggggca
      841 agagatttcc atctgcgtca cgcgccgggc ctttaaagta gttactttgt atagtaaact
      901 tgccatcttc tccggtcact cccgtcctca cttgccccgc cgaatcggtg acggcgacgt
      961 ttgcaccgat gagaggttta aatgaaaaat tgtcaagaat ttgaccggtg atttggcgtg
     1021 taattccaga gccctcgtca ccctcgatat acatcaccaa attacccata tccactttca
     1081 agttgggtaa tgtaatcacg gttgcataat cggtggattg aaattcagtt tttgctaaat
     1141 ggacttgaaa cccttcaccc cgcttcacgt tgtcgatttt gaatgtaaaa ttacctttat
     1201 tactgctggt tttagtatat gtcacgcctt ggtacgttgt cgtcaccgtt acaccttgga
     1261 tggccatacc gtcggtgccg accactttac ccacaatttg gccttcgcca taaacacccg
     1321 ggagcgcttt ttctgaggta aaacaattca gcatcaaaag tggcgctaac accgcgaagc
     1381 ttacatattt tattctatta aaaaccatag ataccaaact taaatcctgt acttgcataa
     1441 ataccctgct ctgccatttc tttagtatta aaaagacgac tttctacttt accaaaagta
     1501 tcggttaacc ccctaaagta ttgtccctca aaaaagaaaa tggcagatcc taagcgcacc
     1561 tcaaaaccaa tgcccgccat cacccccgcc gagaccggtt ttgtaaattt tgcggtgtca
     1621 taaaatgggt cgttgggatc gcccacgttg gggtcttgga tttctaactg ggcagaaagc
     1681 ggagtcttga gaaatccccc tatttgtaaa ttgcctgcaa tcgccttaaa atacttcgcc
     1741 accgggcgca taaattttat tttactcaaa acccttgccg gcgaatagcg ataggaaaac
     1801 attagatgca tgattaaata atccgaacgc atataaccgg tgatcggcga tggcacattg
     1861 ccctgcgcgg gaatgtacgt ggtttgctcg tcgatgacat acgagcggtt ttgttggtat
     1921 gctaaatcga acacaatacc gtaaatgcgg ttgnnnnnnn nnnagagttc gctacgcaga
     1981 ccaatgaccg gcgaaatttg ccaactgtta ccattttcca tggccgtttc cactcttgct
     2041 ttttgcgcgg cggtcaaacc ggcggtcgat atatcgaccc cctcgttggg acccatcgcg
     2101 ttcatgccga caagaaggcc catataaagt gtacgtttat acgattcggg gcctttcgag
     2161 gtcgtgtaat cctgttccgc gtccgatgca gcggttgtgg attgcactga cgccggttgt
     2221 gccggtatcg accgccgcaa ttggattttc ccctgtgcat ggagggggat taaaaaaata
     2281 aataataata ttatagggac tgtgccccta taaaattgtt tatatttatg ttttagcacc
     2341 cgcaccccgc tggcgtatat cccgcgccgg ttgtggctgc cgtgaaccgg aaacatttat
     2401 tgttggcgcc cgcaacacct gcattgccgc tactggcacc caacgcacca cccgaggcgg
     2461 ggttgatggt attccctgta ctacaggtgg gtgcaccacc cccaaaaaat
//
LOCUS       DAOMPD010000399         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16667_length_2509_cov_2.258354, whole genome shotgun sequence.
ACCESSION   DAOMPD010000399 DAOMPD010000000
VERSION     DAOMPD010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16667_length_2509_cov_2.258354"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLY93_11940"
     CDS             complement(<1..399)
                     /locus_tag="PLY93_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70236.1"
                     /translation="MVETFLSSKCPLCAAVGFPHLQKKDAHRKNYKIHRCSQCTVWYI
                     HPMPTSDTLNELYQNTYFKKRSDRGYADYTSEKIYTSVTQTLHKNLTDLNFFAWAKTR
                     PQKRLLEVGCAAGHAVDYFVRRGWEAIGIDI"
     gene            complement(403..1845)
                     /gene="rsmG"
                     /locus_tag="PLY93_11945"
     CDS             complement(403..1845)
                     /gene="rsmG"
                     /locus_tag="PLY93_11945"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRP70237.1"
                     /translation="MPKNILFSLLREIPQVTDEKVAMLLTHWQMMVASDDRTDLTRLE
                     RTRDIAIKHYLDSALPLKFIKLPSPLLDIGTGAGFPGLVLKILSPETHVILGEVRPKR
                     VAFLKSVIDALELTGVQVYPHRIGPQFPLEVQGVVTRALESARDTLYRVATFLPQGGQ
                     VILLKGPKGAEEISEALAALPNFKHEKTYRYRLANTSHARQLVVFTRVKESIRTKNVE
                     KFSPVENSATNKGIPQSLEITSRANNIFKLVASLNESRTIKREERFIVSGKKIIDDLL
                     RNPNAIPFAETLILGQRMHVENSTLKRIYLADALFREVDTLGTKQPLLLMRTPPLQAW
                     QGDSVDGITVFLPFQDPANIGAAIRSAVAFGAQRVVLLKEAASPFLPKAVRACAGSIF
                     NTTILEGPSISELVENPIVLLALDMEGIPIDKVPTTKSVSLLVGVEGYGYKNTGNTTR
                     ITIPISKNIESLNAQSALAIALYELSRRMV"
     gene            1933..>2509
                     /locus_tag="PLY93_11950"
     CDS             1933..>2509
                     /locus_tag="PLY93_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRP70238.1"
                     /translation="MFDAMPILTAKNAFDYARISKIVLSGKNIVRFLAPLVVFEDNHL
                     LVIFKPAGILSQSDKTGDLSVNEIFAAYLKEKYQKPGRVFCAAVQRLDRPASGLMALA
                     RTSKAAARLSEEIRATRFKKRYRVFTEKKLIGGPRAVLTAAMEKQSTKSGFKATLQTQ
                     NNSSLYVVSTRLVRSQHGVHEYDVSIDTGKFH"
BASE COUNT          600 a          570 c          668 g          671 t
ORIGIN      
        1 gatgtcgata ccgatggctt cccaccctct ccgaacaaaa taatccaccg cgtgaccagc
       61 cgcacacccc acttcgagca aacgtttttg gggacgagtt ttcgcccacg caaaaaaatt
      121 taagtcggtt aagtttttat ggagcgtttg cgtgacggat gtatagattt tttcggaagt
      181 gtagtcggcg tatccacgat cgctgcgctt tttaaaatac gtgttttgat agagttcgtt
      241 gagagtatcg cttgtgggca taggatggat gtaccagacc gtgcattggg agcagcggtg
      301 gattttataa ttttttcgat gcgcgtcttt tttttgtagg tgtggaaatc ccacagcggc
      361 gcacagtgga catttgcttg aaaggaacgt ttctaccaca cattatacca tccgtcgcga
      421 aagttcatag agtgcaatgg cgagcgctga ttgcgcgttg agcgattcga tgttttttga
      481 aatgggtata gtgatgcgcg ttgtgttgcc ggtatttttg taaccatagc cttccacccc
      541 cacgagaaga gacaccgatt tggtggtggg tactttatcg atgggaattc cttccatgtc
      601 gagggcaaga agcacaatgg gattttccac aagttcggaa attgatgggc cttctaaaat
      661 tgtggtgtta aaaatcgaac cggcgcatgc acgcaccgct ttgggtaaaa aaggcgacgc
      721 tgcttctttt aggagtacaa cgcgctgcgc accgaacgcc accgccgaac ggatcgcggc
      781 gccgatgttt gcagggtctt ggaacggcag aaaaacagtg atgccgtcga cactgtcccc
      841 ctgccatgct tggagtggcg gtgtgcgcat gagtaaaagt ggttgcttgg tgccgagtgt
      901 atccacttcc cgaaaaagag catcggcgag atagatccgc ttgagcgtgg agttttcgac
      961 gtgcatcctt tgccccaaaa tcaacgtctc tgcaaaaggt attgcgttgg ggtttcttaa
     1021 aaggtcgtcg atgatttttt tcccgcttac aataaaacgc tcttcgcgtt taatcgtacg
     1081 cgattcgttc agtgaagcga caagtttgaa aatattgttc gcgcgtgagg tgatttcaag
     1141 actctgcggt attcccttgt tcgtggcaga gttttccaca ggagaaaact tttctacatt
     1201 tttagtgcgg atggattctt tcacgcgggt aaagacgacg agctgccgtg catgggaggt
     1261 gttcgccaaa cgatagcggt atgttttttc atgcttaaag ttggggagag cagcgagtgc
     1321 ttctgaaatt tcttctgcac ccttgggtcc cttaagaaga atcacctgcc ccccttgcgg
     1381 caaaaaagtg gcaacgcggt agagagtgtc gcgcgcgctt tcaagagcgc gggtgacaac
     1441 gccttggact tcgagcggaa attgcggacc gatgcggtgt ggatagactt ggacgccggt
     1501 gagctctaat gcatcgataa cgctcttaag aaaagccacg cgtttaggac gcacctctcc
     1561 caagatgacg tgcgtttcgg gcgagagaat tttgagcaca agcccgggga aacctgcacc
     1621 ggtgccaatg tctaaaagcg gcgacggcag tttgatgaat ttgagcggca gcgccgagtc
     1681 caaatagtgc tttattgcaa tgtcacgcgt tctctccagc cgcgttaagt cggtgcggtc
     1741 gtcggaggca accatcattt gccaatgcgt caagagcatg gcaacttttt catcggtcac
     1801 ttgcggtatt tcgcgtagga gcgaaaagag tatattttta ggcattaacg gcgagggatt
     1861 attttcgtcc tctgcgttat ttttttttga aaatggggtt ggtcgcttat ctctcttttt
     1921 cacggggggt atatgtttga cgcaatgccg atactcacgg caaaaaacgc gttcgattac
     1981 gcccgtatat cgaaaatcgt tttatcgggg aaaaacattg tgcgtttcct tgccccgttg
     2041 gttgtctttg aggataatca tctgctcgtt attttcaaac cagcgggtat tttatcgcag
     2101 agcgacaaga ccggtgattt gtccgtgaat gaaatttttg ccgcatatct caaagaaaaa
     2161 taccaaaagc ctggtcgagt gttttgtgcc gctgtgcagc gtctggatag acctgcgtcc
     2221 gggcttatgg cgcttgcacg cacttccaaa gcggcagcgc gactttcgga agaaattcgc
     2281 gctactcgtt tcaaaaaacg gtaccgcgtt tttaccgaaa aaaaactcat cggtgggcca
     2341 cgtgcagtgc ttacagcagc tatggaaaaa cagagcacaa agagtggttt taaggctact
     2401 ctccaaacgc aaaataattc ctcattatat gtagtctcca cacgcttggt gcgctcgcaa
     2461 cacggcgtcc acgaatacga cgtgagcatc gacacgggga aattccacc
//
LOCUS       DAOMPD010000400         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16719_length_2503_cov_2.839052, whole genome shotgun sequence.
ACCESSION   DAOMPD010000400 DAOMPD010000000
VERSION     DAOMPD010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_2503_cov_2.839052"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /gene="mutS"
                     /locus_tag="PLY93_11955"
     CDS             <1..1481
                     /gene="mutS"
                     /locus_tag="PLY93_11955"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRP70239.1"
                     /translation="GLSNNPTVRHLGQVRDTLGAILRVQKFIADNRLEPLLESGWRSL
                     EDFPTVVYSLLEDALLAENLPPLLDSRRFVKFGYMPLLDELIEISENAVTHLTQYEEK
                     QKAEHGITTLKIKHNNVLGYFIEVSKGQISKVPQSYQRRQTLTNGERYTTQELKELEN
                     KILSASEEVLRLQKGVFEDIRTQILENATLLQNWAERLARLDVLLAFARTAVTHRHVR
                     PTIHTGKRLRAEAVRHPVIEAYFKSEMFIPNDILLSNENNPTGENEPHLAIITGPNMA
                     GKSTYIRQIGIMQIMAQAGAFVSALSATLPIVDRVFTRIGAHDRLSHGESTFFVEMAE
                     CARIFRNATEDSLILLDEVGRGTSTYDGISIAQAMIEELNDASKGRPKTLFATHYSEL
                     SHLISKENGIIGLTVSVIEKEGEVFFMRKIVEGVAEKSFGIHVADMAGMPKSVTRRAQ
                     EILMNLEKKADFLLAEDNYARGEKRPTRPAEIFDDKQGELFE"
     gene            complement(1470..>2503)
                     /locus_tag="PLY93_11960"
     CDS             complement(1470..>2503)
                     /locus_tag="PLY93_11960"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRP70240.1"
                     /translation="WTGLNGRSFIPLLSSFACAIPGILSARAMPNGRARMATILIAPL
                     MSCSARLPVYVLFIGAFIEPRAGALWAAVALFGMHLVGITVALPLAWLLNRKVLKAGE
                     GTFMLELPEYRLPHWPNVFRRVYDAGSDFIKTVGTIVFALSIVIWALVYFPHSEKTYN
                     KELSAFAQSSQLNTTSLTKILNKKKPTEKEKTIKADFDNTLRAAYLRDSLMGRFGRTI
                     EPIFEPLGFDWKISVAVLSAFPAREVFVSTLGIIFSVTDAEENSTALGKKLVNEKKSD
                     GTPAYSILVAISIMVFFALCAQCMSTLATIRRELMSTRWAVTVFVVLTSLAYLASLGV
                     YQIGRLLLE"
BASE COUNT          694 a          611 c          621 g          577 t
ORIGIN      
        1 ctggtttgag taacaatcca acagtgcgtc acttggggca ggtacgcgat acattgggcg
       61 caatattgcg tgtacaaaaa tttatcgcag acaatcgact cgaaccactt ttggaaagtg
      121 gttggcgttc actggaagat ttccccacag tcgtatattc acttcttgaa gatgcgttgc
      181 tcgccgaaaa tttaccccct ttactggaca gtcgccgttt tgtcaaattc ggttatatgc
      241 cgcttttgga tgaattgatt gagatcagcg aaaatgccgt tacgcactta acccagtacg
      301 aagaaaaaca aaaagcagag catggaatta cgacactcaa aataaagcat aacaacgtgc
      361 ttggatattt tattgaagtc tccaaagggc aaatatctaa agtacctcag agttatcaac
      421 gccggcaaac gctcacaaat ggtgagcggt acaccacgca agagctcaaa gaattagaaa
      481 ataaaatcct gtctgcatcc gaagaagtgt tgcgtttaca gaaaggcgtg tttgaagata
      541 ttcgcacaca aattttagaa aatgcaacgc tactgcaaaa ctgggctgaa cgtttagcgc
      601 gcctcgacgt gcttttggcg tttgcgcgta cagcggtgac tcaccgtcac gtacgcccta
      661 ctatacacac aggcaaacgc ctgcgcgccg aagcggtgcg ccaccccgtt attgaagcgt
      721 atttcaaaag tgaaatgttt atccccaacg atattctact cagtaacgaa aataatccca
      781 cgggtgaaaa cgagccccac cttgccatca tcaccggccc caacatggcg ggtaaaagta
      841 cgtacattcg acaaataggt attatgcaaa ttatggcgca ggcgggtgca ttcgtgtctg
      901 cgctttcggc gacactgccg attgttgatc gcgtgttcac gcgcatcggt gcgcacgacc
      961 gactctcgca cggtgagagt acattctttg tggaaatggc cgagtgtgcg cgtatttttc
     1021 gcaatgcgac agaagattct cttattctgt tagacgaagt tggtcgtggt acgtcaacat
     1081 acgacggcat ttcgattgca caggcaatga tcgaagagct aaacgatgcg agcaaggggc
     1141 gccccaaaac gctttttgcg acacattatt cagagctatc gcaccttatc tcgaaagaaa
     1201 acgggattat aggactcacc gtctcagtca tcgaaaaaga gggagaagtt tttttcatgc
     1261 gcaagattgt cgaaggtgtt gccgaaaaat cgtttggtat ccatgttgcc gatatggcgg
     1321 ggatgcccaa aagtgtcacg cgacgcgcac aagaaattct gatgaacctc gaaaaaaagg
     1381 cggactttct tttggccgaa gataactatg cgcgtggaga aaaaaggccg acccgcccag
     1441 cagaaatttt tgatgacaag caaggcgagt tattcgagta agagtcgccc gatttgatac
     1501 acacccaaag acgctaaata cgccaacgac gtcaacacca caaaaacagt aaccgcccat
     1561 cgcgtgctca tgagttcgcg gcggatggtc gccaacgtgc tcatgcactg tgcgcagagc
     1621 gcaaaaaata ccatgattga aatcgccacc agaatactat acgccggtgt gccgtcggat
     1681 ttcttttcgt tcacgagttt tttacccagt gcggtggaat tttcttcggc gtcggtgacg
     1741 ctgaagataa tgccaagcgt cgatacaaac acctcgcgtg cggggaaagc cgagagcacc
     1801 gcgaccgata ttttccagtc gaatcccagc ggttcaaaaa tcggttcaat cgtgcggcca
     1861 aagcgaccca tcaagctgtc gcgtaagtat gcagcgcgta aagtattgtc gaaatcggcc
     1921 ttgattgttt tttctttctc tgtgggcttt tttttattca ggattttggt gagcgatgtc
     1981 gtgttgagtt gagaagattg agcaaaggcg gatagttctt tgttatacgt tttctcgctg
     2041 tgcggaaaat agacaagcgc ccatatcaca atcgaaagtg caaagacaat tgtgcccacg
     2101 gttttaataa aatcgctccc cgcatcgtat acacggcgga acacattcgg ccagtgcggc
     2161 aaacggtatt cgggcagttc gagcataaat gtgccttcgc ctgctttcaa gactttacgg
     2221 ttgagtagcc acgctaaagg cagcgcaacg gtgatgccca caaggtgcat cccaaagagg
     2281 gctaccgccg cccacagtgc ccccgcacgc ggctcgatga acgcaccgat aaaaagaaca
     2341 tacaccggta gccgcgccga gcacgacatg agcggtgcga tgagaattgt cgccattcgc
     2401 gcacggccat taggcatcgc ccgtgctgat aaaatccccg ggatggcgca cgcaaaactg
     2461 gaaaggagcg ggataaacga gcgtccattg agaccagtcc atc
//
LOCUS       DAOMPD010000401         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16753_length_2499_cov_2.282324, whole genome shotgun sequence.
ACCESSION   DAOMPD010000401 DAOMPD010000000
VERSION     DAOMPD010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16753_length_2499_cov_2.282324"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..571
                     /locus_tag="PLY93_11965"
     CDS             308..571
                     /locus_tag="PLY93_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70241.1"
                     /translation="MKAAYATVEKVRTALIEAAKTAYEEAGMMGLCAEGRWENALGAM
                     QSISLNNFFKKSATERGKSHRAVSDTPRSKRAIQTTTKTKNKA"
     gene            complement(496..1590)
                     /locus_tag="PLY93_11970"
     CDS             complement(496..1590)
                     /locus_tag="PLY93_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRP70242.1"
                     /translation="MISQRNHALDFLRGITILLMVVVNNQGDWTHVYPLLRHATWHGF
                     LGADIVFPLFLFVAGYAAALKIERTNTIASAYFLSLSRRAALLFSIGLFLNGWPFGIL
                     GGTEFHLSTLRLLGVLQRIALCVFFGGVILWHIRSVRITILSVACLFIFYEIGMRVCI
                     GEYNGQRFGQSFELFTNYARFVDTALLPERMLYRVQGIPFDPEGLFTTLTATASFLLG
                     ALCFRMKKGRLLATAILFFMVAAILPVEPINKQLWTLPYTLLTGATAFLLLTMLERLK
                     WDVPHFWQRPILAMGKNPLMLYALSVLIAKTLALGKVSDNLSLKAHIYGAFTQLPITP
                     ETASLCYALFLVFVVVWMARLLRGVSLTAR"
     gene            1662..2456
                     /locus_tag="PLY93_11975"
     CDS             1662..2456
                     /locus_tag="PLY93_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRP70243.1"
                     /translation="MQRLKNLFLNDRFIFSLILLNTICVFIEGFHSFPAKVIFAAIGI
                     DYAITVLFIVELLIKVKTFSWKGYTAHGWNRFDMVLVLFSMGSLALPFTHWTTQDLSF
                     LIVLRVARVFKFFRFLRFVPGIEQLVQGVQRALKTSVVVLIGFFVFIVVCALISNNLF
                     KEISPEYFGDPAQSMYTIFRVFTIEGWYEIPDALIQNQQGAIVFFIRFYFVVLLTTGG
                     LFGFSIVNSVFVDAMVADNNDALEKKVDDLMRELRSLRQIIEKQNR"
BASE COUNT          691 a          587 c          601 g          620 t
ORIGIN      
        1 cgctaccgca gcgttgtcga tgatacggtt acccaccatt tcaaccgtgt ctttatcggc
       61 actctggttg tggtgtgcga gttctgccat gaggtatgca agttgttttt ctttcggcaa
      121 ttgttccttt tcggggtgta ctttcaaatc aatcgttctc atgacaaacc tcgccactaa
      181 aagaatacac aaaacgaacg aggatttcag aaaaaagaat cgtgttgtca gcgattagta
      241 aactttaaag cctgtgcacc aactttgcga ttgccgcaca aactcatcgt ttggaatctg
      301 cccatgcatg aaggccgctt acgcaaccgt tgaaaaagtc cgtactgcgc tcatcgaggc
      361 agcaaagaca gcgtacgaag aagcagggat gatgggctta tgcgcggaag gccgttggga
      421 aaacgcactc ggagcaatgc aaagtatcag tctgaataac ttcttcaaaa aaagcgctac
      481 agaacgcggg aaatctcatc gcgccgtgag cgacacgccg cgcagcaaac gtgccatcca
      541 aaccaccaca aaaactaaaa acaaagcata acaaagcgac gccgtttcag gcgtgatggg
      601 taattgagta aacgcgccgt aaatgtgcgc tttgagcgag aggttatcgc ttacttttcc
      661 caacgcgagc gtctttgcga taagcacgct gagtgcgtag agcataaggg gattttttcc
      721 catcgccaag atgggtcttt gccagaaatg gggtacatcc cacttgaggc gttcgagcat
      781 agttaataac aaaaacgccg ttgcgccagt gagcaacgtg tacggcagtg tccagagttg
      841 tttattgatg ggctcgacag ggaggattgc cgctaccatg aagaataaaa ttgcggtggc
      901 aagaaggcgt ccttttttca tccgaaaaca aagcgcgcct aaaagaaaag aggcggtggc
      961 tgtgagtgtg gtgaaaagcc cttcggggtc gaatggaata ccttgtacgc ggtagagcat
     1021 cctttcgggc aaaagcgcgg tgtcgacaaa gcgggcgtag ttggtgaaaa gctcaaaact
     1081 ttggccgaaa cgctggccgt tatattcccc aatgcaaacc cgcatcccga tttcgtagaa
     1141 aataaacaag cacgcgaccg agaggatagt tatcctcacc gagcgtatgt gccacaaaat
     1201 cacccccccg aaaaaaacgc agagcgcgat gcgctgcagc acgcctaaaa gccgcaacgt
     1261 gctcaaatgg aactccgtac cgcctaaaat accgaacggc cacccattca aaaaaagacc
     1321 gatggagaaa aggagagctg cacggcgcga aagcgacaga aaataagcgg aggcaatggt
     1381 gttcgtacgt tcgattttga gcgcggcggc gtaaccggca acaaacaaaa aaagcggaaa
     1441 aacaatgtcc gcgcctaaaa agccgtgcca tgttgcgtgg cgtaaaaggg gataaacgtg
     1501 cgtccagtcg ccttggttgt tcaccactac catgagaagg atagttatcc cgcgcagaaa
     1561 atcgagcgcg tggttacgtt gggatatcat aggagcctga ccacatacca actctacaac
     1621 ttaattttct ttgtagagtc aaaaaataat atggtagtgc aatgcagcgg cttaaaaatc
     1681 tttttctcaa cgaccgattt attttttcac tcatccttct taacaccatc tgtgttttta
     1741 tcgaaggctt tcactccttt ccagcaaaag taatctttgc cgccataggc attgactatg
     1801 ccattaccgt actttttatc gttgagcttt taataaaagt aaaaacattt tcatggaaag
     1861 ggtacaccgc acacggttgg aatcgcttcg acatggtgct cgttcttttt tcgatgggct
     1921 cgcttgcgct accgtttacg cattggacta cacaagattt aagtttcctc attgtgttac
     1981 gcgttgcccg agtattcaaa ttttttcgtt tcctccgctt tgtgcctggc atcgagcaac
     2041 tcgtgcaagg ggttcagcgc gcgctcaaaa cctcggtagt tgtgctcatc ggtttttttg
     2101 tgtttatcgt cgtgtgcgcg cttatctcaa ataatctttt caaagaaatt agtcccgaat
     2161 actttggcga tccggcacag tcgatgtaca cgatatttag agttttcacc atcgagggct
     2221 ggtatgaaat tcccgacgcg ctaattcaaa accagcaagg tgcgatagtt ttttttatcc
     2281 gtttttactt cgttgtgctt ttaacaaccg gcgggctctt tggattttcc atcgtgaact
     2341 cggtgtttgt cgatgcaatg gtagccgaca ataacgatgc gctcgaaaag aaggtagacg
     2401 acctcatgcg cgaattacgt tcattgcgac aaattataga aaaacagaac cgctaatttt
     2461 tcggagcgag agggattcga accctcgata cggttaccc
//
LOCUS       DAOMPD010000402         2498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16759_length_2498_cov_2.194433, whole genome shotgun sequence.
ACCESSION   DAOMPD010000402 DAOMPD010000000
VERSION     DAOMPD010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16759_length_2498_cov_2.194433"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLY93_11980"
     CDS             <1..1373
                     /locus_tag="PLY93_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRP70244.1"
                     /translation="KFFWQNSARKTLYSKKVKFLYKMTMVYFLKRLYAVLFLLLYSSF
                     AWGSEAQSVVNELTLDAAESMFLSANYQLLAGKFQIDAAQAQVLQARLWTNPNITFAQ
                     SVYTPASRQFFDFRNTGETQVQLEQLFLLAGKRGYQIKAAQLNAANAEQNFYDLLRTL
                     RIELQSNFYGIHFMRENIAFYDEGIAGLKNTVLMLETAYKKRLVLLSEVMRIRTLLFS
                     LQNERTELVATLIAREHNLKTLLGMPIENTQAIIPKVQSEMQQSFSNRLIARDEALKR
                     AKDNRPDLKMAINAVFLEENNLALQHALKYPDITVGANWDRAGPFTENYFGLQVTAPL
                     PTLNRNQGNIATAEKNLNVVKELAIKTQRDIERDVMIAYERAVEKDKLYQEFKDKFNT
                     EYQNLVGLMFKNYQKRYITVIEFADFYEAYRNTITTMIKLEEERVASFAVLAAAVNAP
                     VLRSEP"
     gene            1370..2332
                     /locus_tag="PLY93_11985"
     CDS             1370..2332
                     /locus_tag="PLY93_11985"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRP70245.1"
                     /translation="MKKTFGILFFLSSLLHCGSQLNGSATPVAAIEKPTVFDNGNEII
                     FPPDYLGLKRIHTQKIQKGNGSFSITAPAKIMAAIQVQKNSRIALFESQELTTLYAQH
                     RTARINAQRAARSLSRTKDMFESQVATQREVIDAESEAASTRAAVSEMEARMRAYGIN
                     PKEIESVHRDTVWLIADIPEAQISEVRKGQKVKIIFASLPGEEKQGRAEAISDNIDPM
                     TRTAKIRISVANPKRVLKSGMFAEVNFGETKSNFITLPADAVITVENQNYVFVKRKGN
                     EFIRRAVTTLPSRTNFVAIASGISRDDEVVVHGAILLKGLSFGY"
BASE COUNT          728 a          608 c          574 g          588 t
ORIGIN      
        1 aaaaattttt ttggcaaaat agcgcgcgca aaacgttgta ctcaaagaaa gttaaatttt
       61 tatataaaat gaccatggta tattttttaa agcgtctata cgccgttctg tttcttcttt
      121 tatatagttc ttttgcatgg gggagcgaag cccaatcggt tgttaacgag ttaactctcg
      181 acgcggcgga aagcatgttt ctgtcggcga attaccagtt actcgccggt aaattccaaa
      241 tcgacgccgc gcaagcgcaa gttttgcaag cgcggttgtg gacaaaccct aacataacct
      301 ttgcacaaag cgtatacacc cccgcatcgc ggcaattctt cgatttccgc aacaccggtg
      361 aaacgcaagt ccaattggaa caactatttt tactcgcggg taaacggggc tatcaaatca
      421 aagcggctca actcaacgct gctaacgccg agcaaaattt ttacgacttg ttgcgcacgc
      481 tgcgcatcga attgcaatcc aacttttatg gcattcattt tatgcgcgag aatatcgcct
      541 tttacgatga gggaattgcc ggccttaaaa atacggtttt gatgttagaa accgcgtaca
      601 aaaaacggct cgtactttta agcgaggtga tgcgcatccg tacgcttctt ttttcgttac
      661 aaaacgaacg caccgaactt gtggcgacac tcattgcacg cgagcacaat ttgaaaacgc
      721 tgttggggat gccgattgaa aatacgcaag cgattatccc caaagtgcaa agcgagatgc
      781 aacaaagttt tagcaaccgc ctcatcgccc gcgacgaagc gttaaagcgc gcgaaagaca
      841 accgccccga cttgaaaatg gcgattaacg cggtgttctt agaagaaaat aatttagccc
      901 tgcaacacgc gctcaagtac cccgacatca ccgtcggcgc caactgggac agagcgggac
      961 cttttaccga aaattatttt ggacttcaag tcactgcgcc gctgcccaca ctcaaccgca
     1021 accaaggcaa tatcgccacc gctgaaaaaa atctaaacgt cgtcaaagag ctcgcgataa
     1081 aaacacaacg cgacatcgaa cgcgatgtaa tgattgcata cgaacgcgcc gttgaaaaag
     1141 ataaactcta tcaagaattc aaagacaaat ttaataccga atatcaaaat ttagtagggc
     1201 ttatgttcaa aaattatcaa aaacggtaca tcaccgtaat cgaattcgcc gatttttacg
     1261 aagcgtaccg caacaccatc accacgatga ttaagctcga agaagaacgg gttgcgtcgt
     1321 tcgcagtgct cgccgcggcg gtgaacgcgc cggtgctaag aagcgaaccg tgaaaaaaac
     1381 attcggaatt ttattttttt taagttcgct tttacattgc ggctcgcagc tcaacggttc
     1441 ggccacgccc gttgctgcga ttgaaaaacc gaccgtgttc gataacggca atgaaattat
     1501 ttttccaccg gattacctcg ggttaaagcg catccatact caaaaaatac aaaaaggcaa
     1561 cggttcgttc tcaatcaccg ctccggcaaa aattatggcg gctatccaag tacaaaagaa
     1621 tagtcggatt gctctttttg aatcgcaaga actcacaacg ctctacgcgc aacaccgcac
     1681 tgcgcgtatt aacgcccagc gcgccgcccg atctttgtcg cgaaccaaag atatgtttga
     1741 aagtcaagtc gcgacgcagc gcgaagtcat cgatgcggaa agcgaagcgg catcgacgcg
     1801 tgcggcagtg tcagaaatgg aagcccgtat gcgcgcgtac gggataaatc ccaaagagat
     1861 tgagagcgtg caccgcgaca ccgtgtggct tattgccgac atccccgaag cgcagataag
     1921 cgaagtacgc aaaggacaaa aagtaaaaat aatttttgcg tcgttgcctg gggaagaaaa
     1981 acaaggcaga gcagaagcga ttagcgacaa catcgacccg atgacgcgta ctgccaaaat
     2041 tcgcattagc gtggcgaatc ctaaacgcgt gcttaaatcg ggcatgtttg ccgaggtaaa
     2101 ctttggcgaa acaaaatcga attttatcac gttgcccgcc gatgccgtca tcactgttga
     2161 aaatcaaaat tatgtttttg tcaaacgcaa aggaaacgaa tttatccgcc gcgcagtaac
     2221 aacgctcccc agccgcacaa acttcgtcgc gattgcatcg ggaatctcac gcgacgacga
     2281 agttgtggtg catggggcaa tcttgctgaa aggcttgagc ttcggctatt agagctcgat
     2341 tgtggttttc aaacttcttg agatttcgct gaagtatcga ttgccgacga tttttctcgc
     2401 ggcgtttatt ttctttgccg gtatttttgc atgggtcgat atgcgcaaag aggcataccc
     2461 cgatataggc gatacgatgg tgagcgtttt ggcgacat
//
LOCUS       DAOMPD010000403         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16793_length_2493_cov_2.720673, whole genome shotgun sequence.
ACCESSION   DAOMPD010000403 DAOMPD010000000
VERSION     DAOMPD010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16793_length_2493_cov_2.720673"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLY93_11990"
     CDS             <1..501
                     /locus_tag="PLY93_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HRP70246.1"
                     /translation="RKVAEGGLTRLKSMTVCESALKQVADTLHLGELLRMGKGEITTG
                     GLTRVSNLADAMEATIGAIYVGAGYEAAQKFVLKHFHAILENPELVDGNQDFKSTLQE
                     YLAKKSHLHPEYKLIAADGPDHEKEFTIALFVNGVKETEGVARTRKQAEQNAAREYMS
                     RVKERK"
     assembly_gap    778..787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <788..1128
                     /locus_tag="PLY93_11995"
     CDS             <788..1128
                     /locus_tag="PLY93_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70247.1"
                     /translation="FLRVVERKRKFRRKVTRQYNWGNGHCVIVKVRFTPKEYDSLHTM
                     AAGIRVSVSYLIYDLIQFWEKASKKSAKFAVGGNYAPKMLEWSKIRVKFEEKLSFRRA
                     GNRFFAPLRL"
     gene            complement(1138..>1787)
                     /locus_tag="PLY93_12000"
     CDS             complement(1138..>1787)
                     /locus_tag="PLY93_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70248.1"
                     /translation="LIRGWKTLRLRLAELDLKNQVALSLGKGKETPLEKAADDYVYSL
                     TVLRDDIMSEHLPYIAINVSSPNTPNLRLLQNAKAIRMLVEKCVAVSPRPVVVKFAPD
                     FNSIKEYRQTLDSALQGGAGGIIVTNTTTNHSLAPLPEELKNFGGGLSGKPLAELGRT
                     YLKETLKITRGKVPVISSGGIFSPEEARHRIDMGADLVQVYTGFIFYGPNFAVNA"
     assembly_gap    1788..1797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1798..2132)
                     /locus_tag="PLY93_12005"
     CDS             complement(<1798..2132)
                     /locus_tag="PLY93_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRP70249.1"
                     /translation="MLQQAYRLTKPLLFQLNPERTHGAVDLLMRMITPFLRGNSRHDR
                     DAFILFDRKFYARIGIAAGFDKFCRAPDYLFKLGFGFIESGSLTPLAQKGNSRPRLVR
                     LPEHEALIN"
     gene            complement(2154..2450)
                     /locus_tag="PLY93_12010"
     CDS             complement(2154..2450)
                     /locus_tag="PLY93_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70250.1"
                     /translation="MWPNPKCDKPENLTGTLVPDPITINGKRRSVGGQICNCHHAQSQ
                     SFLDLLQADHNLENEEVGRKSKQAYDMTEGDKVMKQLGGELPVPGAATMPEKRP"
BASE COUNT          646 a          576 c          631 g          620 t
ORIGIN      
        1 cgcaaagttg ccgagggggg gcttacccgc cttaaatcaa tgacggtgtg cgagtcggcg
       61 ctcaagcaag tggccgatac gctccacttg ggcgagcttt tgcgcatggg caaaggcgag
      121 attaccaccg gtggcctcac ccgtgtttcc aacctcgccg acgcgatgga ggccaccatc
      181 ggcgccatct acgtcggtgc gggttatgaa gcggcgcaga agtttgtcct caaacacttc
      241 catgcaattt tagaaaatcc agagttagtc gacggtaatc aggattttaa gtcgacgttg
      301 caagaatacc tggcgaaaaa aagccactta caccccgagt ataaactcat tgccgctgac
      361 ggccccgatc atgaaaaaga attcaccatt gcgctcttcg tgaacggcgt caaagaaacc
      421 gagggggtgg cgcgtacgcg caagcaagcc gagcaaaacg ccgcgcgcga gtatatgagt
      481 cgagtgaaag agagaaaata atttagtcga atgttcgtaa gcactacctg cggacagaat
      541 cagaaaattt agttacacat aatcccatat aagggataac tatcccaaca gagaattttg
      601 gcctccaaaa tcatgatttt tttcgaaaat ttgcattttt tttctaaaaa gtgagaaaat
      661 ttgaatattt tttggtattt aatatataga gaggcaaaca tgttaaagac atccataagc
      721 ttaccaaaag aatctgtgac gacttggact caaaaccact gggcgatcat gcagatgnnn
      781 nnnnnnnggt ttttacgggt cgttgagcgg aagagaaagt ttcgtcggaa ggtaacacgg
      841 cagtataact gggggaatgg gcattgtgtg attgtaaaag tgcggtttac gcccaaggag
      901 tatgactcat tgcatacgat ggcagcgggc atacgcgtga gtgtatcgta tcttatttat
      961 gatctcatcc aattctggga aaaagcgtcg aaaaaaagtg ctaaattcgc cgttgggggt
     1021 aactatgcgc ccaagatgct ggaatggtca aaaatcaggg taaaatttga agaaaaacta
     1081 tccttcaggc gggcaggcaa ccgttttttc gcaccactac gactttagcg gcgctaatca
     1141 agcattaaca gcaaaatttg gtccataaaa aatgaatccc gtgtaaacct gcactaagtc
     1201 ggcgcccatg tcgatgcggt gccgtgcttc ctcgggtgaa aaaattcccc ctgatgaaat
     1261 caccggcact ttgccacgcg tgattttcag tgtttctttc aggtaagtac gccccaactc
     1321 cgccaatggc ttacccgaaa ggccgccacc aaaatttttt aattcctcgg gcaatggcgc
     1381 cagcgagtgg ttggtggtcg tgtttgtgac gataatcccg cccgcgccgc cttggagcgc
     1441 agagtctaaa gtttgtcgat attctttgat ggaattaaaa tcgggagcaa acttgacaac
     1501 cacgggacga ggagaaacgg ccacacactt ttcaaccaac atacggattg ctttggcgtt
     1561 ttgcaaaagg cgtaagttgg gcgtgttggg cgaactcacg ttaatggcga tgtacggcag
     1621 gtgttcgctc atgatgtcgt cgcgcaatac cgttaaagaa tacacataat cgtcggcggc
     1681 tttttccagt ggtgtctctt tgcctttgcc taaactcaac gccacttgat tttttaagtc
     1741 caattcggcc aaacgcaacc gcaaagtttt ccagccacgg atgagccnnn nnnnnnnttg
     1801 ttgatgagtg cttcgtgttc ggggagacgc accaagcgtg ggcgggaatt tcccttttgg
     1861 gcaagtggcg ttagactccc tgactcaata aaaccaaagc ctagcttgaa gagataatcg
     1921 ggtgctctgc aaaatttatc gaaaccagcg gcaatcccaa tgcgtgcata aaattttcga
     1981 tcgaataaaa taaacgcatc acggtcgtgc ctcgaattac cacgtagaaa tggcgtaatc
     2041 atccgcatga gtaaatccac cgcaccgtgc gttcgttcgg gattaagctg gaaaagaagc
     2101 ggtttggtga gtcggtaagc ttgctgtaac accgcgttgt tatttatggc gcgttatggg
     2161 cgcttctctg gcattgtggc cgcaccgggt acgggtaact caccacccaa ctgtttcatc
     2221 accttgtcgc cttcggtcat gtcatacgct tgttttgatt ttctacccac ctcttcgttt
     2281 tctaaattgt ggtcggcttg taataaatca agaaagctct gcgattgggc gtggtggcag
     2341 ttacatatct ggccgcccac cgagcggcgt ttgccgttga tggtgatggg gtcggggacc
     2401 aacgtgccgg tcaaattttc tggtttgtcg cacttggggt taggccacat gccggtttag
     2461 cggcacacac cacccgcgtg cacacctttg ggt
//
LOCUS       DAOMPD010000404         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16843_length_2487_cov_2.713405, whole genome shotgun sequence.
ACCESSION   DAOMPD010000404 DAOMPD010000000
VERSION     DAOMPD010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16843_length_2487_cov_2.713405"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /gene="nuoL"
                     /locus_tag="PLY93_12015"
     CDS             <1..1675
                     /gene="nuoL"
                     /locus_tag="PLY93_12015"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRP70251.1"
                     /translation="VMMGVVLFVSLTVLIFSVGYLADRKKDYARFYAWVCFFMFSMLL
                     LATAANLMVFFIGWELVGVSSYKLISFYMDREDAQRAGIKAFILNRIGDAAFLVAMFA
                     IFRVTGTADFRELAFAFSKGSIATANANFIGLMIFIAVAAKSAQIPLYTWLPDAMTGP
                     TPVSALIHAATMVTAGIFLMLRLESIFVVAPEASNIVAWTGGITALLAAFVAVAQTDI
                     KKVLAFSTVSQLGLMVLAVGTGSYVAAFFHLFTHAFFKAMLFLGAGAVIVYRHHEQNI
                     RRMGGIVKAHPFLAILFWLALIALAGLPGLSGFFSKDHIMAAAFSSQKGGLLLGTIAV
                     AASLLTAFYSFRLGILVFHGKDRLNAAHDNHHGDLPSVSFYLPLVLLVVPTVLAGYIG
                     VPAFFTGYEPVFFEKLTINLPASTMEFFKVARVALDHHAELLVVAASIAVAIVGALAA
                     IVYYGILRKVPVAEGAYHRTLSRLSLKKLYIDEIYHALFVNPYKKLGDKIAAWDFNVL
                     PKISDGIGLLTQSVCTLVARLQSGELAHYAAYMVAGVALLLFIAMGTLA"
     gene            1943..>2487
                     /locus_tag="PLY93_12020"
     CDS             1943..>2487
                     /locus_tag="PLY93_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP70252.1"
                     /translation="MLAALFSWSTFKEQGVRQHKVYWAMLLFLESTILGVFSAWNLIL
                     FYAFWELFLIPVVYLIWRFGLEERKRAAMQFFLYTFLSSVFMLVAFAAFVYYTPRIGV
                     NFDLRQNIAQDTQFLSVTHQRLVLLFLMLAFLVKMPLVPFHSWLPLTHTQAPIGTLLL
                     SGLILKLGSYGVLRFVTPNMQA"
BASE COUNT          478 a          599 c          650 g          760 t
ORIGIN      
        1 ggtgatgatg ggcgtggtgc tttttgtttc gcttactgtg ctgattttca gcgtcggtta
       61 tttagccgac cgcaaaaaag attacgcgcg cttttacgca tgggtgtgtt tctttatgtt
      121 tagcatgctg cttttggcga cagcggcaaa cttgatggtc ttttttatcg gctgggaact
      181 cgtgggtgtt tcgtcgtata aactcatctc cttttatatg gaccgcgaag acgcacaacg
      241 cgcggggatc aaagcgttta ttttgaaccg catcggtgat gcggcctttt tagttgcgat
      301 gtttgcgatt ttccgcgtca ctggtactgc cgacttccgc gagttggcct ttgccttttc
      361 taaaggcagt atcgccactg cgaatgccaa tttcatcggt ctcatgattt ttatcgctgt
      421 ggcggccaag tcggcgcaaa tccctcttta tacatggcta cccgatgcga tgaccggacc
      481 cactccggtg tcggcgctca tccacgcggc gacgatggtg acagcaggta tttttttgat
      541 gctgcgctta gagtctattt ttgtcgtcgc tcccgaagca tcgaacatcg tcgcttggac
      601 gggaggcatc actgcgcttt tggcggcgtt tgtcgccgtc gcacagacag atataaagaa
      661 ggtactcgcg ttctcaacgg tctcacagtt ggggctcatg gtgcttgccg ttggcacagg
      721 ttcatacgtc gcggcgtttt ttcatctttt cacgcacgca ttctttaagg cgatgctctt
      781 cttgggggcg ggtgcggtga ttgtgtaccg tcaccacgaa caaaatatcc gccggatggg
      841 aggtatcgtc aaagcacatc cttttttagc gattctcttt tggcttgcgc ttattgcatt
      901 ggcaggactt cctgggcttt cgggtttttt ctcaaaagac cacatcatgg cagcggcgtt
      961 ttcgtcgcaa aaaggcggac tcctcttagg gacgatcgcc gtcgctgctt cgcttttgac
     1021 ggcgttctat agttttcggt taggcatttt ggtcttccac ggtaaagata gactcaacgc
     1081 ggcgcacgat aaccaccacg gcgatttacc gtcggtgagt ttttatttac cgttagtact
     1141 tttagttgtg cctaccgtgc tcgcaggcta cattggcgtg cccgcatttt tcaccggtta
     1201 tgaaccggtg ttctttgaaa agctgacgat aaacttgccc gcaagtacca tggagttttt
     1261 caaagtggcg cgtgtggcgc tcgaccacca tgcggagtta ttagttgtcg ctgccagtat
     1321 cgccgtggca atagtggggg cgttggcggc gattgtttac tatggtattt tacgcaaagt
     1381 gccggtggct gaaggcgcat accaccgcac tttgagccgg ttgagtctca agaaattata
     1441 tatcgacgag atataccacg ctctttttgt aaatccgtat aaaaagttgg gcgataaaat
     1501 tgcggcgtgg gatttcaacg tgcttcctaa aatttcagac ggcattggtc ttttgacgca
     1561 aagtgtgtgc acgttggttg ctcgactaca aagcggtgag ctcgcccatt acgccgcgta
     1621 tatggtggcc ggtgttgcgc tcctcctttt tatcgctatg gggacactcg catgattgct
     1681 atttcaattc tcgtacccag cgctgtcgcc gttttactcc ttttattacg cgatgaaaaa
     1741 aatgtggtga cgtacaaaaa ccgcaacgcg tggctcacgg tgggcgctac cgtagtgtcg
     1801 cttatcgcag cgttggcgat gacaaaacag tttgtattca ctgcgagcac accgcaattc
     1861 catgaagtgt ggtggcaagg tggctcgtcg gggctcaatc tcacctttgc ggtggatggc
     1921 ctttctctac cgctttttgt ggttgctagc tgcccttttc tcgtggagta ctttcaaaga
     1981 acaaggcgtt cgacaacaca aagtgtattg ggcgatgcta ctttttttag agagcaccat
     2041 tttgggtgtt ttttctgcgt ggaatttaat tcttttttat gctttttggg aattattttt
     2101 aattccggtg gtctacctaa tatggcgatt cggtttagag gaacgcaaac gcgcggcgat
     2161 gcagtttttt ctctatacat tcctctcgtc cgtgtttatg ctcgtggcgt tcgcagcgtt
     2221 cgtgtattat acgccgcgta tcggcgtcaa tttcgacctg cggcaaaaca ttgcgcagga
     2281 cacgcaattt ctcagcgtaa cgcaccaacg tttagtgctt ctctttttaa tgctcgcgtt
     2341 tttggtcaaa atgccgttgg tgcctttcca ctcgtggctg ccgctcacgc acacgcaagc
     2401 ccccatcggc acgttgcttt tgtcggggct cattctcaaa ttgggctctt acggcgtgtt
     2461 acgttttgtc acgccgaaca tgcaggc
//
LOCUS       DAOMPD010000405         2485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16863_length_2485_cov_2.183128, whole genome shotgun sequence.
ACCESSION   DAOMPD010000405 DAOMPD010000000
VERSION     DAOMPD010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16863_length_2485_cov_2.183128"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLY93_12025"
     CDS             <1..760
                     /locus_tag="PLY93_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP70253.1"
                     /translation="TFFFGHLIVNLSLYLAVAAIYDSFPKYTGREWHSGRVVAIAWNV
                     VLVVVLLAYFHHLYMDFVQPKALQYIGQVASYISGLPSAVVTIFGAMAIVYRAPVKWS
                     LGFTLLFFGMMGWGIGGIGAVIDSTIAVNSKFHNTLWVPAHFHSYMIEGLVLMALGYF
                     YHYCQEKAQLPENPRFQKLIVWLMLVGGYGFLLMFYLSGAKAIPRRFAFYPAELSHGA
                     LFSAIGLVFISAFLLGLILYIYETGKRWKKARAL"
     gene            766..1521
                     /locus_tag="PLY93_12030"
     CDS             766..1521
                     /locus_tag="PLY93_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70254.1"
                     /translation="MKAILFLIVVVLFAPTDANSTGNNNAENILREESKNLYRSVPDI
                     VISTIDGKRVSLSALNKEIPLLVTLFYRNCPGACSPFLRSLKSASEGNGGLGHDYKIV
                     ALSFDAEDDLDDIRAMAKAIGVEGNPGWVLGTAAPRDILKLDDAIGFWFKRNTAQKEF
                     DHPSLVAAVKDGKVQRVLLGISVNPVRFQETIFALKGIFVPFYEQPGNDTIFRCLEYD
                     PKTGTMGFGWGMLVLAIPAFFALLIAVVIFRRR"
     gene            complement(1563..>2242)
                     /locus_tag="PLY93_12035"
     CDS             complement(1563..>2242)
                     /locus_tag="PLY93_12035"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770541.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRP70255.1"
                     /translation="MLRRKKQSFGGKTCIVSGSGNVAIYTIEKIHELGGKVIACSDSN
                     GVIIDKNGIDLDFVKQIKEVERRRIKAYTEKHTDAIYQPSGKIWEIPCQVAMPCATQN
                     ELNGKDAEKLVKNGCIAVGEGANMPTTPEGIRVFLESGILYGPGKATNAGGVATSALE
                     MQQNASRDSWSFEYTEKRLAKIMSNIHELCYETAEEFGTPGNYVNGANIAGFMKVGQA
                     MLSMGLI"
     assembly_gap    2243..2252
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2253..2466)
                     /locus_tag="PLY93_12040"
     CDS             complement(<2253..2466)
                     /locus_tag="PLY93_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRP70256.1"
                     /translation="MTELFRHIGEYTDVPAGDIGVGGREIGYMFGQYKRITNRYESGV
                     LTGKGLHYGGSLVRTEATGYGAVYFVS"
BASE COUNT          561 a          596 c          578 g          740 t
ORIGIN      
        1 aactttcttt tttggtcacc tcattgtcaa cttgagtctt tacttggcag ttgcggcgat
       61 ttacgattca ttccccaaat ataccggccg cgaatggcac tcgggacgcg ttgttgcgat
      121 tgcgtggaac gtggtgctcg tggttgtgct ccttgcttat ttccaccacc tttatatgga
      181 cttcgtgcaa cccaaagcgt tgcagtatat cggccaagtt gcttcttata taagtgggtt
      241 accctcggcg gtggtaacca tctttggcgc gatggcaatt gtgtaccgcg caccggttaa
      301 gtggtcgctg ggcttcaccc ttctcttttt tggtatgatg gggtggggga ttggcggtat
      361 cggcgctgtc atcgactcga caattgcggt caacagcaaa ttccacaaca cgttatgggt
      421 gcctgcacac tttcatagct atatgattga agggttagtg ctcatggcgc tgggttattt
      481 ctaccactac tgccaagaaa aagcgcaact gcctgaaaat ccacgctttc aaaaactcat
      541 cgtgtggctt atgctcgttg gcggttatgg cttcttgctc atgttctatc tttccggagc
      601 gaaagcaatc ccgcgtcgtt ttgcttttta ccccgcagag ctctcgcacg gtgcgctttt
      661 ctcggcgatt ggcttggtgt tcatcagcgc cttcttgttg ggattgattc tttacatcta
      721 cgaaaccggt aagcgctgga aaaaagcacg ggcgttgtaa acgtattgaa agcgatactt
      781 tttcttatcg tagttgtact gtttgcaccg accgatgcaa acagtacggg aaataataat
      841 gcagaaaata ttctgcgtga agaatcgaag aacttgtatc gcagcgtccc cgacatcgtt
      901 atttcaacca tcgatggtaa acgtgtttcg ctgtctgcgc tcaacaaaga aatcccgctt
      961 cttgttactc tcttttatcg caactgtccg ggcgcgtgca gtcctttttt gcgttcactc
     1021 aaaagcgcat ctgaaggaaa tggcggttta gggcacgatt acaaaattgt agcgttaagt
     1081 tttgatgctg aagacgattt agacgacata cgcgctatgg caaaggctat tggcgtcgaa
     1141 ggtaatccgg gttgggtctt gggtaccgcc gcccccagag atattctgaa actcgacgat
     1201 gcgattggct tttggtttaa gcgaaacacc gctcaaaaag aattcgacca cccttcgctc
     1261 gttgctgcgg taaaagacgg caaagtgcag cgggtacttt tgggtatttc ggtaaaccct
     1321 gtgcgttttc aagaaacgat ttttgctctc aaaggtattt ttgtaccgtt ctatgaacaa
     1381 ccaggtaatg atacaatttt tcgttgtttg gagtatgatc caaaaaccgg tacaatgggt
     1441 tttggttggg ggatgctcgt tttagctatc cccgcttttt ttgcactcct gatagcagtc
     1501 gttatcttcc gccgccgtta attatcctta acgtgcgcaa agcgcacgta ggatagttca
     1561 acttaaatca gtcccatcga gagcatcgct tggcctactt tcataaagcc tgcgatgttg
     1621 gcgccgttga catagttacc cggcgtacca aattcttcgg cggtttcata acaaagttcg
     1681 tgaatgttgc tcataatttt agccagtcgt ttttcggtat attcaaacga ccacgaatcg
     1741 cgtgaagcat tttgttgcat ctcaagcgct gaggtcgcta caccgcctgc attcgtcgct
     1801 ttgccaggac cgtacagaat accactttcc aaaaagactc gaataccttc gggggtcgtc
     1861 ggcatattgg cgccttcacc cactgcaatg caaccattct ttacgagttt ctcagcgtct
     1921 ttgccgttga gctcgttttg tgtagcgcag ggcattgcta cttggcaagg gatttcccaa
     1981 atttttccac tgggctgata aatcgcatcg gtgtgctttt cggtatacgc tttgatacgg
     2041 cggcgttcga cttctttgat ttgtttcaca aaatccaagt cgataccgtt tttatcaata
     2101 atcactccgt ttgaatcaga gcaggcaatg actttaccgc ccaattcgtg gattttttct
     2161 atcgtataaa ttgccacgtt tccagaacct gaaacgatac aggttttgcc gccgaaactt
     2221 tgtttttttc tccgtagcat ttnnnnnnnn nncagaaacg aaatataccg ctccataacc
     2281 tgtcgcttcg gtgcgtacca acgaacctcc gtagtgcaag cctttacctg tcaatacacc
     2341 cgattcgtag cggttggtaa tccgtttata ttggccgaac atataaccaa tttcacgacc
     2401 acccacaccg atgtcgcctg ccggtacgtc ggtgtattcg ccgatgtgcc ggaaaagttc
     2461 ggtcatgaag ctttggcaaa agcgc
//
LOCUS       DAOMPD010000406         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16884_length_2482_cov_2.597033, whole genome shotgun sequence.
ACCESSION   DAOMPD010000406 DAOMPD010000000
VERSION     DAOMPD010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16884_length_2482_cov_2.597033"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLY93_12045"
     CDS             <1..324
                     /locus_tag="PLY93_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRP70257.1"
                     /translation="IEDMPQAYSDADTIVARCGSGTLFEILWAAKPAYLIPYPFAAAN
                     HQRANADAIKPHLNCEIFDERPFNAEHALVRFTAFLKNLPRTPRNPPEARAEEKIIRY
                     IKESL"
     gene            328..>741
                     /locus_tag="PLY93_12050"
     CDS             328..>741
                     /locus_tag="PLY93_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70258.1"
                     /translation="MRIFFSPLKYSFLSSLFLFSTTLFADRWLILPPRLEMPQAKLSK
                     DEAASLARSMALYLRVSRVEKIVQVAETEACLKNANVDMAQKVAPNALKRVAKNCMAE
                     RMLLTRIRSKKSGVEAMTKVYYEESDRLTDTIVTNG"
     assembly_gap    742..751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <752..1909
                     /locus_tag="PLY93_12055"
     CDS             <752..1909
                     /locus_tag="PLY93_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70259.1"
                     /translation="IPDALGKNFSERFSMSPAKPKATSRDLIVVGDTFGSAYFDWPQL
                     KNLLLSVDSQKSAFCFSNAQGQVQALRPVGDKALQNQFLDKLHFFGGNTYNDALFDCA
                     FKALNVSREEGREAQIVFFVSDYPRASNTQITLRSQMRRLVRNARILIAPASNATSAM
                     LLFWQRIAREAGDKTQFIPLMQRVRIGLSSGQEWFLFRRGSRLYESRTEEKERFDDGI
                     VIPDKYLSSLTNENLVATYQELSQNKVISQNAVESYINPVQKSLSRIFQSATKTPSAW
                     RVLLEQNGQRYYLSLTPSLAQKLRLQEFARVYVELLPSTSSDVILNRPSPALLVDAVD
                     SSPSDEIDVSDYIRYPQKYLRKAIGGRSFYILPGKVLQVLSPENDALESDF"
     gene            1930..>2482
                     /locus_tag="PLY93_12060"
     CDS             1930..>2482
                     /locus_tag="PLY93_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP70260.1"
                     /translation="MNFTLLKNKNFLFFIGARTAMVLTFQIVGTIAQWQVYAATRDSL
                     YIGFLALTEVIPAILMNLVGGYAADTFSRKRMALIGSCFTTTSVFILVSTSGSHHLES
                     IVPLFVATFVNGLGRGIVAPASTALLGDIVDRNFYARAAVFNSIAWQMSIISGPAMAG
                     IVYEKFTPLLCYQIALALTCLSML"
BASE COUNT          631 a          622 c          541 g          678 t
ORIGIN      
        1 atcgaagata tgcctcaagc gtatagcgac gccgacacca tcgtcgcgcg ttgcggcagc
       61 ggtactcttt ttgaaatcct gtgggcggca aaaccggcat acctcatccc ctaccccttt
      121 gccgctgcaa accaccaacg cgccaacgcg gatgcgataa agccacatct caactgtgag
      181 atttttgacg aaagaccgtt taatgccgaa cacgcgttag taagatttac cgcgtttcta
      241 aaaaatttac cgcgtacacc aagaaatcct ccagaggctc gagccgaaga aaaaattatc
      301 cgatatatta aagagagctt gtaatctatg cgtatatttt ttagtccttt gaaatactcg
      361 ttcctttctt ctctatttct tttttccaca actctttttg cagaccgttg gctcatcctc
      421 cccccaagat tagaaatgcc acaagcgaaa ctctctaaag acgaggcggc gtctctggcg
      481 cgctcgatgg cgctatacct gcgcgttagc cgcgttgaaa aaattgtaca agtcgccgaa
      541 accgaagcgt gtcttaaaaa tgccaatgtc gatatggcgc aaaaggttgc gcccaatgcg
      601 ctcaagcgtg ttgcaaaaaa ttgtatggct gagcgcatgc ttttgacgcg catccgatca
      661 aaaaaaagcg gagtggaagc gatgacaaaa gtctattacg aagaaagcga ccgcctcact
      721 gacactatcg taacgaacgg cnnnnnnnnn nattcccgat gcgctgggta aaaatttcag
      781 cgagcgtttt tccatgtctc ccgcaaagcc caaagcgaca agtcgcgacc tcatcgttgt
      841 gggggatact tttggttcgg cgtactttga ttggccgcaa ctcaaaaatc ttttgttgag
      901 tgtcgattcg caaaaaagcg cattttgttt ttccaacgca caaggccaag tgcaggcgtt
      961 acgcccggtt ggcgataagg cattacaaaa tcaattcctc gacaaactcc atttcttcgg
     1021 tgggaatact tacaacgatg cactcttcga ttgtgcgttc aaagcgctga atgtttcgcg
     1081 tgaagaaggc agagaggcgc aaattgtttt ttttgtttct gactaccccc gcgcctcgaa
     1141 tacgcaaatc acattgcgct cgcaaatgcg ccgcttggta agaaatgcgc gtattttgat
     1201 tgcgcctgca agcaatgcca cgagcgccat gcttcttttt tggcagcgta tagcgcgtga
     1261 agcaggagat aagacacagt ttattccatt gatgcaacga gtgcgcattg ggctttccag
     1321 cggccaagag tggtttttat tccgccgtgg ctctcgcctc tacgaatcac gcacagaaga
     1381 aaaagagcgc tttgacgatg ggatagttat ccccgataaa tatctctcct cgctcaccaa
     1441 cgaaaatcta gtcgccactt accaagaact ttctcaaaat aaagtgatct cacaaaatgc
     1501 agttgagtcc tatattaacc ctgtgcaaaa atcgctttct cgtatttttc aaagcgccac
     1561 taaaacacca tcggcgtggc gtgtgctttt agaacaaaat ggccaacgct actatctctc
     1621 gcttaccccc agtcttgcgc agaaattacg cttgcaagag tttgcgcgtg tctatgttga
     1681 gcttttgccg tcgacttcct ccgatgtaat tctaaaccgt ccaagccctg cgctccttgt
     1741 cgatgctgta gattcctctc cgagcgacga aatcgatgtg agcgactata tacgctatcc
     1801 acaaaaatat ttacgtaaag ccattggagg ccgttcgttt tatattcttc caggaaaagt
     1861 tttgcaagtg ctctccccag aaaacgatgc gttagaaagt gatttctaag agcatacttt
     1921 ttcatgagca tgaattttac ccttcttaaa aataaaaatt ttttattttt tattggagcg
     1981 cgtactgcaa tggtgctcac gtttcaaatc gtcggcacca tcgcacagtg gcaagtgtat
     2041 gcagcgacgc gcgattcact ctacatcgga tttcttgcgc tcaccgaggt tatacccgca
     2101 attttaatga atctcgtggg tggttatgcc gccgacacat tcagccgcaa acgcatggcg
     2161 ctcatcggtt cttgctttac caccaccagc gtatttattt tagtttctac atccggctct
     2221 catcatttag aatcgattgt gccgcttttt gtagcgacgt tcgtcaacgg cttgggacgc
     2281 ggtattgtcg caccggcgag tactgcactt ttgggcgaca ttgtcgatag aaatttttat
     2341 gcacgcgctg cggtctttaa ttctatcgca tggcaaatgt cgattatctc tgggcctgcg
     2401 atggcgggaa tcgtatatga aaaatttacg ccacttctct gttaccaaat tgcattggcg
     2461 ctcacttgcc tttcgatgct ct
//
LOCUS       DAOMPD010000407         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16939_length_2475_cov_2.640909, whole genome shotgun sequence.
ACCESSION   DAOMPD010000407 DAOMPD010000000
VERSION     DAOMPD010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16939_length_2475_cov_2.640909"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLY93_12065"
     CDS             complement(<1..690)
                     /locus_tag="PLY93_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRP70261.1"
                     /translation="MAKELYELNEAIPLGEIPKKMHAQVIREDRFGEPKKAFQREVVD
                     VPDINADEALVLVKAAGINYNNIWASLGIPLNMVEVHRKVQNDQSGFHIGGSDASGIV
                     YKVGANVKNVKVGDEVVVHCGSWDVNNADIKAGKVDPIVHPSNAIWGYEVNYGSFAQF
                     TKVQGHQLLPRPKHMSWEESAAYMLVGATAYRMLMGFKEHTVRKDDVVLIWGGAGGLG
                     GDIGVALMLVGA"
     gene            782..1573
                     /locus_tag="PLY93_12070"
     CDS             782..1573
                     /locus_tag="PLY93_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804853.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRP70262.1"
                     /translation="MNRLKIGISACFMHKDPTRAIFKGKTLLYNEESLVHWAMRLGAM
                     PLMVPSVVEGEGRVSLADYAYALDGLILHGGSDVAPEAYGEAPMKPEWKGDRVRDLYE
                     IELFHEFKKLGKPILGVCRGAQVINVALGGSLYQDIETQVPDALNHRDWQRYDQNFHE
                     IDIAPQSYLARLYGEGKFKTNTIHHQALKTMGTNLVIEATSSEDGIVEAFKLKEPTYV
                     CGIQWHPEFHDEADRSLLSGDPLLKDFLSECEIFKMNQKMDRAKK"
     gene            1606..>2475
                     /locus_tag="PLY93_12075"
     CDS             1606..>2475
                     /locus_tag="PLY93_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70263.1"
                     /translation="MRAAAGVLLIVSSLILNACGGRSSSPTALVTDENGLTTIATDDF
                     PSDFALTLTQVTVTGNDATVEFTTGVEARVWIEYGKNSYTENKRTAPTTGVTHTLTLT
                     ALEKGRYFFKIGAVSLDDTQKSAWGGNLDASFLIASASLAQISNIQTTTQGEATTITF
                     NTDIAAQASIDVGLTTAYDLLTESESGFTKTHSITVSLPQMGTFYYRIRLVDDAGDTK
                     TDDNAGAGYTFTRANSEPLGSKLNPIVIDTTPLASNTVVNYNHSATTTGAASNYISSY
                     APYTNAGSYAAPEV"
BASE COUNT          627 a          657 c          606 g          585 t
ORIGIN      
        1 cgcgcctacc agcatgagag caacgccgat gtcgccgcca aggccgcccg cgccgcccca
       61 aatgagcacc acgtcgtctt tacgcacggt gtgctctttg aagcccatga gcatacggta
      121 cgcggttgca cccaccagca tgtacgctgc ggattcttcc cagctcatgt gtttggggcg
      181 tggaagaagc tggtgtcctt ggactttcgt aaactgcgca aaactgccgt agttgacttc
      241 ataaccccaa atcgcgttcg atggatgcac gatgggatcg actttacccg ccttgatgtc
      301 ggcattgttg acgtcccatg agccgcaatg caccacgact tcgtcgccga ctttcacgtt
      361 cttgacgttg gcgcccactt tatatacaat gcccgacgcg tctgagccgc cgatatgaaa
      421 gcccgattgg tcgttttgca ctttgcggtg gacttcgacc atgttcaaag ggatgcctaa
      481 agacgcccaa atgttgttgt agttaatacc ggctgcttta acgagcacca acgcttcgtc
      541 ggcgttgatg tcaggcacat cgactacttc gcgttgaaac gcttttttgg gttcaccaaa
      601 gcggtcttct cggatgacct gcgcgtgcat ttttttagga atttctccca gagggattgc
      661 ctcgttgagt tcatagagtt cttttgccat gggcgggaga atttaattta gagacaggcc
      721 gtcaaaaatt ttgatactaa aagtgctaca aggcctgaat cactggtaca aaatgccgca
      781 aatgaatcgt ctaaaaattg gtatatcagc ctgttttatg cataaagacc ccacgcgcgc
      841 catcttcaaa ggaaagacgc ttctttataa tgaagaatcg ttggtgcatt gggcaatgcg
      901 cttgggcgcg atgccgttga tggtcccctc tgtggtagag ggcgaaggcc gcgtatcgct
      961 tgccgattat gcgtatgcac ttgacggtct cattttacac ggtggctctg acgttgcacc
     1021 cgaagcgtat ggtgaggcgc caatgaagcc cgaatggaaa ggcgatcgtg tgcgcgacct
     1081 ctacgagatt gaactcttcc atgaattcaa aaaattggga aagccaattt tgggtgtgtg
     1141 tcgtggcgca caggtgatta acgtcgcgct cggtggctcg ctctaccaag atattgaaac
     1201 ccaagtaccc gatgcactca accaccgcga ttggcaacgg tacgatcaaa attttcacga
     1261 gattgatatt gctccccaaa gctatctggc gcgcctttat ggcgagggaa aattcaaaac
     1321 aaacaccatc caccaccaag cgttaaaaac gatggggact aatctcgtaa tcgaagccac
     1381 gtcgagtgaa gacggtattg tcgaagcgtt caaacttaaa gagccgacgt acgtgtgcgg
     1441 tatccagtgg catcccgagt tccacgacga ggcggatcgc tcgcttttat cgggagatcc
     1501 actcctcaaa gattttttat ccgagtgtga aatcttcaaa atgaatcaaa aaatggatcg
     1561 agcaaaaaaa tgaccctctc ggccaccggc acgcaataat actatatgcg tgcagccgca
     1621 ggcgtgttac ttatcgtctc ctcactcatc ctaaatgcct gcggcggccg ctcttccagc
     1681 cccactgcgc ttgtcaccga cgagaatggt ttaacaacca tcgccactga cgattttccc
     1741 tctgacttcg cattgacact cactcaagtc actgtcaccg gcaacgacgc aacggtcgaa
     1801 tttaccaccg gcgttgaggc gcgggtgtgg attgaatacg gcaaaaacag ttacactgaa
     1861 aacaaacgca ctgcgccaac gactggcgtg acacacaccc tgactttaac tgctttagaa
     1921 aaaggccgct atttttttaa gataggtgcc gttagcttag acgataccca aaagtctgcg
     1981 tggggtggca atctcgatgc gtcgtttctc atcgcatcgg caagccttgc acaaattagc
     2041 aacattcaaa cgacaacgca aggcgaagca accaccatca cattcaacac cgatattgcg
     2101 gcgcaagcat ccatcgatgt gggattgacc acggcgtacg atttactcac tgagagcgaa
     2161 agcggtttca caaaaacgca ttcgataacc gtgtcgcttc cacaaatggg tacgttttat
     2221 tatcgtatcc gtttggtgga tgatgcaggc gatacaaaaa ccgacgacaa cgcaggtgcg
     2281 ggctatacgt tcacacgggc aaactccgaa cctttgggct ctaaactcaa cccgattgtc
     2341 atcgatacaa ctccgcttgc gtccaacaca gtggtgaact ataaccatag tgcgacgacg
     2401 accggcgcgg cgtctaatta tatcagctcc tatgcgccgt acacgaatgc ggggagttac
     2461 gccgcacccg aagtg
//
LOCUS       DAOMPD010000408         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_16973_length_2471_cov_2.276490, whole genome shotgun sequence.
ACCESSION   DAOMPD010000408 DAOMPD010000000
VERSION     DAOMPD010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16973_length_2471_cov_2.276490"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLY93_12080"
     CDS             complement(<1..575)
                     /locus_tag="PLY93_12080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HRP70264.1"
                     /translation="MIHPSKIIHELGIAPLKKLGQNFQIDTHVVANMASLIPSDAKIL
                     EIGPGLGAWTEVFLQRGHSMVLVEKDRTLAARLLTVYENNKKVEVVEGDFLAMPFDVP
                     PISMTTAAVGNLPFYITSELLLRLIADCTFIKYGLFGVQLEVAERIAAAKGSSLAIAL
                     NAHGKLTLAGRIARTSFYPIPAVDAAILFFHR"
     gene            complement(579..1370)
                     /gene="whiG"
                     /locus_tag="PLY93_12085"
     CDS             complement(579..1370)
                     /gene="whiG"
                     /locus_tag="PLY93_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HRP70265.1"
                     /translation="MPTDPKKYDETDEQLLWEEYKKSHEPKLREYFIKKYAPLVKYVA
                     GKVAVGMPHNVEFDDLVSYGTFALLDAIEKYDPAKEVKFKTYAMTRVRGGIFDELRNV
                     DWIPRSIRQKAKQVEKVILELENRMGRTADDEEIAHELDIDIDEFHDLMSKISGTSLI
                     SLNDVWHGNDENEEVVFVDTLESPEALNPDVIVEREEIKNMIVECIKKLPDREKKVVV
                     LYYYEDLTLKEIGEVLEVTESRVSQLHTKAIMRLRGRLSQMKNLM"
     gene            complement(1414..>2471)
                     /locus_tag="PLY93_12090"
     CDS             complement(1414..>2471)
                     /locus_tag="PLY93_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRP70266.1"
                     /translation="SRLKALVVSQWGNYGLPPKQSSFIMHATVVQHYLGGGYYPLGGS
                     HHIFESIKNVILEKGGACLTSHPVTEIIVEDGKAIGVRACAERDSGSPTKEFYADKII
                     SDAGAYNTYLKLLPKVDIPFGGALKQFYKSHPAVGNVTLYLGLRESPTTLGFGGENHW
                     IYSSYDHDANFAASRARPQTGNDWFDENELAGVYLSFPSLKNAHATAHTAEIIAFTDY
                     TFFEKWKEEPWKKRAEEYKQLKEKISQKLIAFVNARYPGFADLIEYRELSTPISTEHF
                     TDHPQGSIYGVPSVPDRFAAAKAPWCNPQSPVENLYLTGADVSSPGVAGAMMGAVATL
                     SHLPNGFKLLDLMRLQP"
BASE COUNT          604 a          614 c          600 g          653 t
ORIGIN      
        1 cgatgaaaaa ataaaatggc ggcgtcaacc gccggtatag gataaaaact cgtgcgcgcg
       61 atacgtcccg ccaaagtgag tttgccgtgc gcgttcaacg caatggcgag tgacgaacct
      121 ttcgcggcag cgatgcgttc ggcgacctca agctgcacgc cgaaaagacc gtacttaata
      181 aaagtgcagt ccgcaatcaa ccgcaacagc aattcactcg ttatataaaa aggtaaattg
      241 cctacagccg cagtggtcat agaaattggt ggtacatcaa acggcatggc gaggaaatcc
      301 ccctcgacga cttccacctt tttattgttt tcatacacag tgagcaagcg tgccgccagc
      361 gttctatctt tttcaacgag caccattgaa tgcccccgct gtaaaaaaac ttccgtccat
      421 gcacccaacc ctggaccaat ctctaaaatt tttgcatcgc taggtatcag cgaggccata
      481 ttcgcgacga cgtgcgtatc aatttgaaaa ttttggccaa gttttttgag aggggcaatt
      541 cccaattcgt ggataatttt agaaggatgt atcaagagct acatcaaatt tttcatttgc
      601 gagagccgtc cccgcaaacg cataattgct ttggtgtgga gttgcgacac ccgcgactcg
      661 gtgacttcga gcacttcgcc aatttctttg agcgtcaagt cttcgtagta ataaagcacc
      721 acgacttttt tttcacggtc gggcaatttt ttaatgcact cgacaatcat gttcttgatt
      781 tcttcgcgct cgacaatcac gtcggggttt aacgcttcgg gcgactcgag ggtgtcgaca
      841 aacacgactt cttcgttttc gtcgttaccg tgccacacgt cgttgagcga gatgagcgac
      901 gtgcctgaaa tttttgacat gagatcgtgg aattcgtcga tgtcgatgtc aagctcgtgc
      961 gcaatttctt catcgtcggc ggtgcgcccc atgcgatttt caagttctag aattactttt
     1021 tccacttgct tggctttttg tcggatagac cgcggtatcc agtcgacgtt gcgtagttcg
     1081 tcaaagatac cgccacgcac ccgtgtcatt gcataagttt tgaacttaac ttctttggca
     1141 ggatcatatt tttcaatcgc atcgagcaaa gcaaatgtgc cgtagctcac caaatcgtcg
     1201 aactcgacgt tgtgcggcat accgacggca accttaccag cgacgtattt aacgaggggc
     1261 gcatatttct taataaaata ttcgcgcaat ttgggctcgt gggatttctt atactcttcc
     1321 caaagaagct gttcatctgt ctcgtcgtac ttcttgggat ccgtaggcat tatgtcaccc
     1381 gtgctctggt ggcgatggct acgtcaagtg ttttcaaggt tgaagcctca ttaaatctaa
     1441 gagcttaaac ccgttcggca agtgcgaaag tgtggccact gcccccatca ttgcgccggc
     1501 caccccggga gaggaaacat cggcgccggt gaggtataaa ttttcaacgg gcgattgagg
     1561 attgcaccac ggcgccttcg cggcggcaaa acggtcggga acgctgggca caccgtagat
     1621 gctaccttgc gggtggtcgg taaaatgctc ggtagaaatt ggcgtggata actcgcggta
     1681 ctcgataagg tctgcaaatc caggatagcg tgcgttgaca aaagcaatca acttttggct
     1741 gattttttct ttaagctgtt tatattcctc tgctcgcttt ttccacggct cttctttcca
     1801 tttttcaaaa aatgtatagt cggtgaaagc gataatttca gcggtatgcg cggtggcgtg
     1861 tgcatttttc aacgaaggaa aagaaaggta tacccccgcg agttcgttct cgtcgaacca
     1921 atcatttccc gtctgtggac gcgcgcggct cgcggcaaag ttggcatcgt ggtcgtagct
     1981 cgaataaatc caatggtttt caccgccaaa acccaaagtg gtgggacttt ctctgagccc
     2041 caaatagagc gtcacgttgc cgaccgccgg gtgcgatttg taaaattgtt ttaatgcacc
     2101 accaaaggga atatctacct tgggcaataa tttgagatac gtgttgtacg caccagcgtc
     2161 ggaaataatt ttatcggcat aaaactcttt agtgggactt ccactgtcac gttcggcgca
     2221 cgcacgcaca ccgatggctt tgccgtcctc gacaataatt tccgtgacag ggtgcgaagt
     2281 gaggcatgcg ccgccctttt caaggatgac gtttttgatc gattcaaaaa tatggtgcga
     2341 accaccgagg ggataataac cgccgcctaa ataatgctgc accaccgttg cgtgcatgat
     2401 gaacgaactt tgtttgggcg gcaaaccata gtttccccac tgcgacacca caagcgcttt
     2461 gaggcgcgaa t
//
LOCUS       DAOMPD010000409         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17084_length_2457_cov_2.380100, whole genome shotgun sequence.
ACCESSION   DAOMPD010000409 DAOMPD010000000
VERSION     DAOMPD010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17084_length_2457_cov_2.380100"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLY93_12095"
     CDS             complement(<1..404)
                     /locus_tag="PLY93_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRP70267.1"
                     /translation="MKVVALANQKGGVGKTTTTINLGAYLASEGKRVLIVDIDPQGNA
                     GSGLGLEIAEEKHTIYGWLLGEEKIAPIQKTKETRLDIIPANIDLSGFEIDVRDAANR
                     DFILKERLATLKEDYDFVLIDCPPSLGVLTINA"
     assembly_gap    577..586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<587..1167)
                     /locus_tag="PLY93_12100"
     CDS             complement(<587..1167)
                     /locus_tag="PLY93_12100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP70268.1"
                     /translation="MLEKNHYPPLSEKITPQNLTRLNAYADLLFSEAMPAGFIGDCTR
                     DDIDTRHILDALWTALAPESCAVFPLASTRPLNVFDLGAGAGLPSLPLAILFSQHTFH
                     LIDAQEKRLRFIAEALPKLGLTNVKLYHCVVQDFTKKFIDAHTADIIIFRAFRKVLAS
                     LELALNVLKAVESGGENPKVLYWRSQKIPFTERG"
     gene            1238..1483
                     /locus_tag="PLY93_12105"
     CDS             1238..1483
                     /locus_tag="PLY93_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70269.1"
                     /translation="MSPELLDILVCPRSKKKLTQADSATIEEINRRIAEGVCVEISGT
                     SVATPTTEGLVQNETGLFYFVREGIPVLIYENAVTYK"
     gene            1499..>2457
                     /locus_tag="PLY93_12110"
     CDS             1499..>2457
                     /locus_tag="PLY93_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRP70270.1"
                     /translation="MVPQLPITDFSRIDSPEALKKLKPTDLPAYCDYLRQFILQSVAQ
                     SGGHLSSNLGSVEITVALHYVFNSPLDRFCWDVGHQCYAHKILTGRKGEFSTIRKTGG
                     LSGFPKRSESTHDLYNTGHAGTAISQAMGESVGLRLMQKEGEPLRNSIAIVGDASIVT
                     GMAFEAMNHAGFARTPMLVLLNDNEMSISQNVGALSYRLTQLINTRFYKKSKRGFIAL
                     MSKIPLIGRVINRLAKRFRMSMKSLAIDHQFFGELGFRYLGPIDGHDVVRMVNVLSSL
                     PPIEEPILLHVVTKKGYGYKLAENDQIECHGVARFDPYYGIEQ"
BASE COUNT          622 a          576 c          600 g          649 t
ORIGIN      
        1 gcgttgatgg tgagcacccc caaactcggt gggcaatcga tgagtacaaa atcataatcc
       61 tctttgagtg tcgccaagcg ttctttgagt ataaaatcac ggttcgctgc atcgcgcacg
      121 tcgatttcaa atccagacaa gtcgatattc gcagggatga tgtcgaggcg ggtttctttt
      181 gttttttgaa tgggcgctat tttttcttcg cccaaaagcc aaccatagat agtgtgcttt
      241 tcctctgcga tttcaagtcc caaaccgctg cccgcattcc cttgtgggtc gatgtcaacg
      301 atgagtacgc gcttgccctc ggaggcgagg taggcgccga gattgatggt cgtggtggtt
      361 ttacccacac cgcctttttg gttggcgaga gcgacgactt tcatgcgtta cgactcaacc
      421 tcgacaagtt tatcggcggt aatttttttc caagagcgag gatagccttt caacgcggga
      481 cgaactcggc taaacgtata aagtccgcgc ggtaaaatag tctctgccga cgtaaacttc
      541 acaaaggaat cgatgttata gcctaaatct gccactnnnn nnnnnncctc gctctgtgaa
      601 aggtatcttt tgagaccgcc agtaaagtac tttcggattt tccccgccgc tttccaccgc
      661 tttcagaacg ttcaacgcca gttcaagcga cgctaaaact ttacgaaatg cgcggaaaat
      721 aatgatgtcc gcagtgtggg catcgataaa ttttttggta aaatcttgca ccacacagtg
      781 ataaagtttt acgttcgtca agcccaattt agggagagct tctgcgataa atcgcagccg
      841 tttttcttgc gcgtcgatga gatgaaatgt atgctgcgag aacaaaatag caagcggcaa
      901 cgatggcaat cccgcgcccg cgcccaagtc aaacacgttt agtggacgag tactcgcaag
      961 cggaaacacc gcgcacgatt ccggggcaag cgcagtccaa agtgcatcca aaatatggcg
     1021 cgtgtcgatg tcgtctcgtg tgcagtcgcc gatgaaacct gcgggcatcg cttccgaaaa
     1081 gagtaaatca gcataggcgt taagccgcgt taaattttgc ggtgtaattt tttcggaaag
     1141 gggaggatag tggttttttt caagcactta ccgcacagca tagatacgtg tcatggcgta
     1201 aagtttgacg cagtgtttca aattccggct ctggggtatg agccccgagc tgctcgatat
     1261 tttggtttgt ccccgttcta aaaaaaaact gacacaagcc gattcagcga cgattgaaga
     1321 aatcaatcgc cgcattgccg aaggcgtatg cgttgaaatt tccggtacgt ccgtcgctac
     1381 gcctacaacc gagggacttg tacaaaacga gacaggcctt ttctattttg tgcgcgaagg
     1441 tattccggtt cttatttatg agaatgccgt gacgtataaa taattttaca ttagggtaat
     1501 ggttccacaa ctaccgatca ccgatttttc tcgcattgat tcacccgaag cgttgaaaaa
     1561 actaaaaccg acggatttac ccgcttactg cgattattta cgacaattta ttttacaaag
     1621 tgtcgctcaa agtggcggac atttatcctc aaacttgggt tcggtggaga ttaccgttgc
     1681 gctgcattat gtttttaatt ctcctttaga tcgtttctgt tgggatgtcg gccaccagtg
     1741 ttacgcacat aaaattttaa caggtcgcaa gggcgagttt agtaccattc gtaaaactgg
     1801 cggcctttcg ggttttccca agcggagtga atcgacgcac gatctttata acactggcca
     1861 cgcaggtacc gccatctcac aagcgatggg cgaatcggtg gggttgcgct tgatgcaaaa
     1921 agaaggcgag ccgttgcgca atagcatcgc cattgtggga gatgcgtcga ttgtcaccgg
     1981 catggcgttt gaggcgatga accacgcagg gtttgcacgc acaccaatgc tcgttctttt
     2041 gaacgacaac gaaatgagca tttcgcagaa cgtgggtgcg ttgagttacc gcttgactca
     2101 actcatcaac acgcgcttct ataaaaaaag taaacgcgga ttcatcgcgc tcatgtcgaa
     2161 aattcctctt atcgggcgtg ttatcaaccg tttggcaaaa cgctttcgaa tgtcgatgaa
     2221 atcgctcgcc atcgatcatc aattctttgg tgaattggga tttcgttatt taggccctat
     2281 cgacggccac gacgtggtac gtatggtgaa tgtgctctca agtctgccac cgattgaaga
     2341 accaatttta ctgcacgtcg tcaccaaaaa aggctatggt tataagctcg ccgaaaacga
     2401 tcagattgaa tgccatggtg tggcgagatt tgacccctat tacggcattg agcaaaa
//
LOCUS       DAOMPD010000410         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17160_length_2449_cov_1.762740, whole genome shotgun sequence.
ACCESSION   DAOMPD010000410 DAOMPD010000000
VERSION     DAOMPD010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17160_length_2449_cov_1.762740"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLY93_12115"
     CDS             complement(<1..949)
                     /locus_tag="PLY93_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRP70271.1"
                     /translation="MSTEKWSGGAVVARMLKLERVEKVFGIIDGTYFGFYASLPKEGI
                     ALVTPRHETSAAHMAGAYARLTGKLGVCMASNGPGVANILPGLVVEEAEGNRVLLITS
                     ARRTGIMYPDRGGTYQCFDQVGVIGKIAKFSVAVPSFERIPEMMRMALRHVYEGRPGV
                     VHVDIPENIMNAKVKTSPTFWPVERYRKVAPLAPTSALVEEAAQMLIQAKLPMIHAGS
                     GVYHADAFAELNEIAHLLQAPVTTSWAARGALVDTSPLAVPMVHVVLNHRVRNAADVV
                     LALGSRIGETDWWGKAPYWKKSSEQKMIQVDTNALQIGGN"
     gene            1113..2255
                     /gene="hrcA"
                     /locus_tag="PLY93_12120"
     CDS             1113..2255
                     /gene="hrcA"
                     /locus_tag="PLY93_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRP70272.1"
                     /translation="MTQRQGEILRALVKEYVTSGEPVGSQILVEKSAMAFSSATVRKE
                     MSVLEQMGLLSSPHTSAGRIPTDRAFQLYLADLVHLFEITLNQKAELENLYRSAALQL
                     DQLLKTTAQMLAQTSNFTGIVLAPNSVGSSIKRIELVSVMENLVLMVMVSSSGAIYEK
                     KIKLDRAVSQEELYKISRYLNQNLKGHELGDVQERGLEFLRTSLNELGELSDIGTAVT
                     QAIVYNPPDQTIHIEGDANLHRKIYEITSDRHKADRIIQQLADKDYLSRIFEKMKNLP
                     QVGSQVGLEIDGEILSGITILGKGYTLSGKSVGALGVIGANRMPYEKIIPALDYSSQI
                     LSNVLGEKTNIEVAGEELKVSEIEITARKELPAQSNRPKNTSVRNK"
BASE COUNT          640 a          654 c          586 g          569 t
ORIGIN      
        1 tattgccgcc gatttgcagc gcgttggtgt cgacctgaat cattttttgt tcgctacttt
       61 ttttccagta cggcgccttg ccccaccaat cggtttcacc gatgcgcgag cccaaggcga
      121 gtacaacatc ggcagcgttg cgcacgcggt ggttcaacac gacgtgcacc atcggcaccg
      181 ctaaaggaga tgtatcgaca agcgcgcccc gcgcagccca actggtcgtc accggcgctt
      241 gcagtaaatg tgcgatttcg ttaagttcgg caaatgcgtc cgcgtggtac acaccgctcc
      301 ccgcgtggat catcggcaat tttgcttgaa ttaacatttg agccgcttct tcaaccagtg
      361 cgctggtggg cgcaagcggc gccacttttc ggtaacgctc caccggccaa aatgtgggcg
      421 acgtttttac tttggcgttc ataatatttt cgggaatgtc tacatgcacg acccccggcc
      481 gcccttcgta tacgtggcgc aacgccatgc gcatcatctc tggaatgcgc tcgaatgaag
      541 gcaccgcaac gctgaacttt gcaattttac caatgactcc cacttgatcg aaacactggt
      601 atgtcccgcc acggtcgggg tacatgatgc cggtgcggcg cgcgctggta atgaggagca
      661 cgcggttccc ttcggcttct tcgacaacaa gcccagggag tatattcgcc acacctggac
      721 cattggatgc catacatacc cccagcttgc ccgtgaggcg ggcatacgcg cccgccatgt
      781 gcgccgcact cgtttcatgc cgaggggtaa caagcgcaat cccttctttg ggtaaagagg
      841 catagaagcc aaagtatgta ccgtcgatga taccaaaaac tttttcgacg cgttctagtt
      901 tgagcatgcg cgccaccact gcgccaccac tccatttttc tgtactcatt cgacactttc
      961 gttatacgaa aatggctttc aactctaatt tttttttcag aggtatttaa attgagcgct
     1021 tgttattttt ttattgccgt tcggtatttc ttttcgtatg ttaatcgcat tgatagcact
     1081 ctgtgtgagc gagtgctatc aaataaacaa ctatgacaca acgccaagga gaaatactac
     1141 gcgctcttgt taaagagtat gtcaccagtg gcgagccggt gggttcgcaa attttggttg
     1201 aaaagtctgc tatggcgttc tcgtccgcca ccgtgcgcaa agaaatgtcg gtgctcgaac
     1261 aaatgggtct tttgagctcg ccacacacct ctgcaggtcg gatccccacc gaccgtgcat
     1321 tccaactcta tctcgccgat ctcgtgcatt tatttgaaat cacgctcaac caaaaagcag
     1381 agctcgaaaa tttataccgc tcggcggcgt tgcagctcga ccaacttttg aaaaccaccg
     1441 cgcaaatgct cgcgcaaacc tctaacttta cgggcattgt acttgctccc aatagcgtgg
     1501 gttcgagtat caagcgcatc gagcttgttt ctgttatgga aaatttggtt cttatggtga
     1561 tggtcagctc gtcgggcgcg atttacgaga aaaaaattaa actcgaccgc gcggtgagcc
     1621 aagaagaact ctataaaatt agtcgttact taaaccaaaa cttgaaaggc catgagctcg
     1681 gggatgttca agagcgcggt ctcgaatttt tgcgtacttc attgaacgag ttgggcgaac
     1741 tttccgacat cggtacggca gtgacgcagg cgattgtgta taaccccccc gaccaaacaa
     1801 tccacatcga gggcgatgcc aacctgcacc gtaaaatcta cgagatcaca tccgaccgcc
     1861 acaaagccga ccgcatcatc cagcaactcg ccgataaaga ttacttatcg cgcatttttg
     1921 aaaaaatgaa aaacttgccg caggtcggct cgcaagtagg attggaaatc gacggtgaaa
     1981 tcttgtcagg catcaccatt ctcggtaaag gctacacctt gagcggcaag agcgtaggtg
     2041 cgttgggcgt catcggcgca aaccgtatgc cttatgaaaa aattatcccc gcactcgatt
     2101 acagttcgca gatcctctcg aatgtactcg gcgaaaaaac aaacatcgaa gtcgccggtg
     2161 aggagctcaa agtgagtgaa attgaaatca ccgcgcgcaa agaactgccc gcacaaagta
     2221 atcgtcccaa aaacacgagt gtgcgcaata aataaaatca ggagttataa cgatgagcta
     2281 agaaacaaac accaacaaag aatatgccac cgaagcgcag gaagcagccg cgatgcaaca
     2341 agaaaaagta gaaagtatgc tcaatgaagc cgaaaccgcc tcggtggatg aaacagatga
     2401 gctcaaaaat caaatcaccg aactcacagg cgctctgcaa cgcgagcgc
//
LOCUS       DAOMPD010000411         2448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17162_length_2448_cov_2.584204, whole genome shotgun sequence.
ACCESSION   DAOMPD010000411 DAOMPD010000000
VERSION     DAOMPD010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17162_length_2448_cov_2.584204"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLY93_12125"
     CDS             <1..525
                     /locus_tag="PLY93_12125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP70273.1"
                     /translation="IPVQSGSKFVLRTMRRGYTPQSFTRRIEHVRKVCPDIHIGTDVI
                     VGFPSESETEFVETMEFCRAANFANIHVFPFSKRRNTPIVDWLERREKMAKDKPMYHE
                     INGNTIRERIGRLIELKNAMGANYAARMEGKTFWAVAEKVEGQNVEYLTENYLRGSLV
                     RDGIKRGERFRVTL"
     gene            complement(515..1561)
                     /gene="lpxD"
                     /locus_tag="PLY93_12130"
     CDS             complement(515..1561)
                     /gene="lpxD"
                     /locus_tag="PLY93_12130"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRP70274.1"
                     /translation="MKLSELAQKLGGHVQGDGNLEIQGVRDLELIRVIDTEAKPQQGF
                     IYFVETKKNFNTHPESIECDALLTTTSLAANFKNAVVVNDKEARLAFIRLLELFEKIP
                     KPLAAEKGSAYIHPTAKVDATAIVMHGAVVLENVVVGAHTVLHPHVVIEPNAKIGAHT
                     VLHPNVVVKYACEIGSHCIIHSSTVIGADGFGFYDKDGTRFKIPQIGNVVVGDYVEMG
                     ASVTVDRATIESTTIGDYTKFDDQVHVGHNCQVGKYVYIAGNTVLAGSVIVEDNVTMG
                     GQSAISGKVRLRKGSVIMGMSATQSDSEPGVIYFGIPARPAREMHKINGSLVYVPELL
                     KRVAALEEKINSKA"
     gene            1707..1901
                     /locus_tag="PLY93_12135"
     CDS             1707..1901
                     /locus_tag="PLY93_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70275.1"
                     /translation="MYELNEGRLLDFQTALKKQPSICETYNVHSAIERGKALLLEPRN
                     VRHQERVEDLFYLLFKRTSG"
     gene            1916..>2448
                     /locus_tag="PLY93_12140"
     CDS             1916..>2448
                     /locus_tag="PLY93_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70276.1"
                     /translation="MKKYYSLSILCFFSFCGAPAVKKVAPNLPLPTTIDAAVQSDFRT
                     PKNRARDIYRHPAETLKFFGLKKNMTVVEIWPSAGWYSEIIAPYLKESGHYYAALPVD
                     GAYSQKLLTWLEDHPQVRGSVTLTKFTPPNHTAIATEESVDMVLTFRNVHNWMKSNAE
                     KEAFAAFFRILKPGGVLG"
BASE COUNT          656 a          631 c          534 g          627 t
ORIGIN      
        1 attcccgtgc agtcgggctc caaatttgtc ctgcgcacga tgcgtcgtgg ttacacgccg
       61 cagtcgttca cgcgccgtat cgaacacgta cgcaaggttt gccccgacat ccacatcggt
      121 accgacgtca ttgtgggatt tccctctgaa agcgaaacag aatttgtcga gacaatggaa
      181 ttttgtcgtg ccgccaactt tgccaatatc catgtttttc cgttttctaa aagaaggaac
      241 acccccatcg tcgattggtt ggaacggcgc gaaaaaatgg cgaaagataa acccatgtac
      301 cacgagataa acggcaacac catccgcgaa cgcatcggcc gcctcattga actcaaaaac
      361 gccatgggag caaattatgc ggcgcggatg gaaggaaaaa cattctgggc ggtcgccgaa
      421 aaagtcgaag gccagaatgt cgagtacctc accgaaaatt acttacgcgg ctctcttgtg
      481 cgcgatggta ttaaacgcgg tgagcgtttt cgtgtcacgc tttagaatta attttttctt
      541 ctaacgccgc cacgcgtttc aaaagttcgg gcacgtacac caacgagccg ttgattttgt
      601 gcatctcgcg cgcaggacgc gcggggatgc caaaataaat caccccaggt tctgaatccg
      661 attgagtcgc gctcataccc ataataacgc tccctttgcg caagcgcacc ttacccgaaa
      721 tcgccgattg accgcccatc gttacgttgt cttcgacaat cacgctaccg gcaagcacag
      781 tatttcctgc aatataaaca tacttaccca cttggcaatt atgccccaca tggacttgat
      841 cgtcaaactt ggtgtaatcg ccaatcgttg tcgattcaat cgtcgctctg tcaacagtga
      901 cgctcgcccc catttcgaca taatctccca caaccacgtt cccaatttgt ggaattttga
      961 aacgcgtacc gtctttgtcg taaaaaccaa aaccatccgc accgatgacc gtcgagctat
     1021 ggatgatgca atgcgaaccg atttcacacg catatttaac gaccacgttg ggatgcagca
     1081 cggtgtgcgc gccaattttt gcattcggtt caatgacgac atggggatgc aagacggtgt
     1141 gcgcaccgac cactacattc tccagcacta ctgcgccgtg catcacgata gcggttgcat
     1201 ccacctttgc tgtgggatgg atatacgcgc tccctttttc tgccgccaag ggtttaggga
     1261 ttttctcaaa aagttcaagt aggcggataa acgccaaacg cgcttccttg tcattgacga
     1321 caaccgcgtt cttaaaattt gctgctagcg aagtggtggt gaggagtgca tcgcattcga
     1381 tcgattcggg gtgcgtgttg aaattttttt tcgtctccac aaaataaata aaaccttgtt
     1441 gtggttttgc ctctgtgtcg ataacgcgga tgagctctaa gtcacgcaca ccttggattt
     1501 caagattgcc gtcaccttgg acatggccac ccagcttttg ggccaattcg cttaatttta
     1561 tcatgcacaa cggaagtgag cgtgttttgt cgctgtcaac caactttaga aaattaaaac
     1621 tgcacaataa attttactca acaaaaagat tttttatgcc gatataatct tagggcacac
     1681 aaaaacaaaa atcgcctaat agacttatgt acgaattaaa cgaaggccgt ctcctcgact
     1741 ttcaaacggc attaaaaaaa caaccatcca tctgtgaaac gtataacgta cactctgcga
     1801 tagagcgcgg caaagcgctt cttttagaac cacgtaatgt gcgacaccaa gaacgtgtcg
     1861 aagatttgtt ttatcttctt ttcaagcgta cgagcggcta aataccgtct cgtatatgaa
     1921 aaaatattat tccctctcaa tcttatgctt ttttagtttt tgtggcgcac ctgctgtaaa
     1981 aaaggtagct cccaatctcc ccttacctac aaccatcgac gctgcagtac aaagcgactt
     2041 tcgtactccc aaaaatcgag cacgcgatat ttaccgccat cctgcagaaa cgctcaaatt
     2101 ttttggcctc aaaaaaaata tgacggttgt tgaaatatgg ccgagcgcag gatggtattc
     2161 cgaaatcatc gcgccttact taaaagaaag cggccactac tacgcggcac tccctgtgga
     2221 tggtgcgtac tctcaaaagt tgctcacctg gttagaagac catccgcaag tgagaggctc
     2281 tgtcacattg accaaattta ctccgcctaa tcatactgcc attgctacag aggagagcgt
     2341 cgatatggtg ttgacgttcc gcaacgtgca taattggatg aaatccaatg cagagaagga
     2401 ggcttttgct gcgttcttta gaatcctcaa acccggtggc gtcttagg
//
LOCUS       DAOMPD010000412         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17216_length_2442_cov_2.466276, whole genome shotgun sequence.
ACCESSION   DAOMPD010000412 DAOMPD010000000
VERSION     DAOMPD010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17216_length_2442_cov_2.466276"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="PLY93_12145"
     CDS             complement(<1..2040)
                     /locus_tag="PLY93_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRP70277.1"
                     /translation="MHEKTGCACTEGAAKKTHDHAHHHTHTNDNSGGAKVKDPVCGMV
                     IDPATAAAHEDYKGETIYFCSTHCAVEFRKSPDAFMSGVPRAQKKAAAGSLYTCPMHP
                     EILQDHPGDCPKCGMALEPTVPSEDSENHEYKDMKKRFWFSALFTLPLFAVAMGDMVP
                     ALAISHRMPAGWFGWLQFILATPVIVWGGLPLWGKAWRSIKNTSPNMFTLIGLGALAA
                     YFFSLVALLFPHIFPDAFKGHNGMVDLYFESAAVITTLVLLGQVLELRARESTGGAIK
                     ALLGLRVKTAHLVGSYGSERDIPLEEVYQGALLRVKPGEKVPVDGALTEGSSFVDESM
                     ITGEPIPVEKKIGHRVTGGTLNTTGSFVMRAERVGSETLLSQIVQMVADAQRSRAPIQ
                     RLADVVAAWFVPAVVLVSVLSFIVWTFWGPEPRLAYAFVNSVAVLIIACPCALGLATP
                     MSIMVGVGAAAKQGILIKNAEALETLGKITTLVVDKTGTLTEGKPSVAAIFANSGFDE
                     KKLLSYAAALEKPSEHPLAQAIVAEAKTQNIPILVAQKFESLTGSGVRAEVDGRTIFI
                     GNELLMQTNGVDLESAAVFIQTHRGNGETVVFLSEASRLVGAIAIADKIKATTPKAIQ
                     TLHAAGISVVMLTGDNATTANAVAQKLGIDDFKADAKPEDKAAYISALIKEGKTVA"
     gene            2150..>2442
                     /locus_tag="PLY93_12150"
     CDS             2150..>2442
                     /locus_tag="PLY93_12150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRP70278.1"
                     /translation="MQRASEQKRQQGRLKVIVNGQYRFKNDGDWMACQVFDISAGGIA
                     IGGKESFYAGDVIELRFSVENYTLFMTIEITNLTGRKAGGKIIDIGNEDKKAL"
BASE COUNT          594 a          631 c          618 g          599 t
ORIGIN      
        1 cgcaacggtt tttccttctt taataagcgc gctgatgtac gccgctttgt cctcgggttt
       61 ggcgtcggct ttgaaatcgt cgatgcccaa tttttgagcc actgcgtttg cggtggtggc
      121 gttgtccccg gtgagcatca caacgctgat gcccgcagcg tgcaatgttt ggattgcttt
      181 cggtgtcgtc gctttgattt tgtcggcgat ggcgatggcg cccacaaggc gcgacgcttc
      241 actgaggaac accaccgttt caccgttgcc acggtgtgtc tggataaaca ccgctgcact
      301 ttccaaatcc acaccgttgg tttgcatgag gagttcgttg ccgataaaaa tagtgcggcc
      361 atcgacttcg gcgcgtacac cactgccggt gagcgattca aatttttgag caacgagaat
      421 agggatgttt tgcgtttttg cttcggcgac gatggcttgc gcgagaggat gctcgctggg
      481 tttttccaac gcagcggcat agctcaacaa ttttttttca tcaaagccac tgtttgcaaa
      541 aattgcagcc accgacggtt ttccctcggt gagcgtgccg gttttatcga ccacgagtgt
      601 ggttattttt cctaacgtct ctaacgcctc ggcgttttta ataagaatac cttgtttcgc
      661 agcggcgccc acacccacca tgatcgacat cggcgtggca aggcccaacg cgcaaggaca
      721 agcgataatg agcaccgcga cgctgttgac gaatgcgtac gcaaggcgtg gctcaggccc
      781 ccaaaacgtc cacacaataa aagaaagaac agagacaaga acaaccgccg gtacaaacca
      841 cgccgccaca acgtccgcca agcgttgaat cggcgcacgg ctgcgttgtg catccgctac
      901 catttgcaca atttgcgaaa gcagtgtttc gctaccgacg cgttcggcac gcatgacaaa
      961 agaaccggtg gtgttgagtg tccctccggt gactctatga ccaatttttt tctccaccgg
     1021 aatgggttcg ccggtgatca tcgactcgtc tacaaaactt gatccttcgg tgagcgctcc
     1081 atcgaccggt actttttcgc caggctttac acgcaacagt gcgccttggt acacttcttc
     1141 taatggaata tcgcgttcgc taccgtacga acccacaagg tgcgcggtct tcacacgcaa
     1201 gcccaagagg gctttgattg cgccccccgt cgattcgcgg gcacgcagtt cgagcacttg
     1261 acccaagagc accaatgtgg tgatgaccgc cgccgattca aaataaagat cgaccatgcc
     1321 gttgtggcct ttgaacgcat cgggaaaaat atgcggaaaa agcaatgcaa ctaaactaaa
     1381 aaaataagcg gcgagcgcgc ctaaaccaat gagagtaaac atattgggag aggtattttt
     1441 tatcgaacgc cacgctttcc cccaaagtgg caatcccccc caaacgataa caggcgtcgc
     1501 taggataaat tgcaaccagc cgaaccaacc cgcaggcatg cggtggctga tcgcaagcgc
     1561 aggcaccatg tcacccatgg cgacggcaaa aagagggaga gtaaaaagtg cagaaaacca
     1621 aaatcgtttt ttcatgtctt tatattcgtg gttttcgcta tcttccgatg gcactgtggg
     1681 ttccaacgcc atgccgcact tggggcaatc gcccgggtgg tcttgtagaa tctcggggtg
     1741 catggggcag gtgtatagcg aacccgctgc cgcctttttt tgcgcacgcg gcacaccgct
     1801 catgaacgca tcgggggatt tacgaaattc aacggcgcaa tgcgtgctac aaaaataaat
     1861 tgtttctcct ttgtagtctt cgtgcgcagc ggcagtggcg ggatcaatca ccatgccgca
     1921 caccggatct tttactttcg ctccacctga attgtcgttc gtgtgtgtgt ggtggtgcgc
     1981 gtgatcgtga gttttctttg ctgcgccttc tgtacaggcg catcctgttt tttcgtgcat
     2041 tctttctccc gagttaaaga caattatata ccaaaatttt tatgtaaaac tttggtcgct
     2101 cattctgctt tccgataata taattatacg ctttgggaaa agccaaaata tgcaacgagc
     2161 tagcgaacag aaacggcaac aaggccgact taaagtcatt gtgaatggtc aatatcgatt
     2221 caaaaatgac ggcgattgga tggcatgcca ggtttttgac ataagcgcag gagggatcgc
     2281 tattgggggc aaagaatctt tttatgccgg cgatgtgatt gagcttcgtt ttagcgtcga
     2341 aaactacaca cttttcatga cgattgaaat cacaaacctc accggtcgca aagcgggggg
     2401 caaaattatc gacatcggca acgaagataa aaaagcgcta ca
//
LOCUS       DAOMPD010000413         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17295_length_2433_cov_2.625315, whole genome shotgun sequence.
ACCESSION   DAOMPD010000413 DAOMPD010000000
VERSION     DAOMPD010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17295_length_2433_cov_2.625315"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1888
                     /gene="purF"
                     /locus_tag="PLY93_12155"
     CDS             416..1888
                     /gene="purF"
                     /locus_tag="PLY93_12155"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRP70279.1"
                     /translation="MSEHSLKRPSEHPKEECGIVGIYNVEEAANHAYLGAYALQHRGQ
                     ESAGIVSSDGEHLYRYAGMGKVAEVFTPTKLRQLQGRHAIAHNRYSTTGASFLRNAQP
                     IRFESRLGTMALAHNGNLTNANSIRHRLGEEGSLFQTTIDTEVISHLVARSRADNFTD
                     ALIEALKQIRGAYSLVVLTADTLYALRDPNGFRPLVMGKKDEGYIFASETCALDIIDA
                     EYVRDIEPGELVIVSPRGVTSLFPFEKASHNLCIFEYVYFARPDSYVFGKSVYNTRLR
                     MGEILAEESPADADLVMPVPDSSSVAALGYSRQSGIPYHMGLIRSHYIGRTFIEPDQK
                     IRDFGAKIKYNAIRSVVEGKRVIVIDDSIMRGTTTRKIVKMLRVAGAKEIHIRIASAP
                     TRHPCYYGIDIPSRSELIAATHSLEEIIKYLRVDSLAYLSLEGMLRGEKETESRGKSG
                     TRGYCTACFDGNYPVSLDENEANYSNQKTLFSEYQVEEHT"
     gene            1977..2390
                     /locus_tag="PLY93_12160"
     CDS             1977..2390
                     /locus_tag="PLY93_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70280.1"
                     /translation="MLIVAGEESGDILGGAIVREALKKKKIVLWGCGGDRMAAAGVKI
                     HYTANELATIGFWEAAKNYRRLTKIVNALVEKAVQNGTKKAWLIDFPGFNLLLAKRLK
                     AKGIAATLVVSPTVWAWKYNRVFKIRDRFERVLCL"
BASE COUNT          620 a          599 c          615 g          599 t
ORIGIN      
        1 aattccgctt ctataaaatc aacgcctttt tgagagggcc gatcaaaaaa aaatagttat
       61 cgattagaaa acaccgcgtc aatcataata ggatttttgg gcggatttaa atcggcgtat
      121 tcaaatccgc agtgcaagaa atttagtcgc tgcccttacg gtcttgatgg tatcaggcta
      181 ctcatggaca gctcttttgc ccgtcgtggg ttttttgatg cgaaagtact gaaacgtact
      241 cggcgtggct gcgatcgcag cgctcttgcg ccaggtaagc gccataccga ataccacggc
      301 gattattact cgtaagatgt tcgccgcact gaatcacagg ccccttcgct ttccgctttg
      361 cggtatgact cgatgcgcat aatttaccgc atccgtgcgt aacaaaaagg acagtttgtc
      421 cgagcattcc ttaaaacgcc cttcggaaca tcctaaagaa gagtgtggca tcgtcggcat
      481 ctataatgtt gaagaagcgg ctaaccacgc gtatttgggc gcatacgcac tccaacaccg
      541 ggggcaagaa agcgcgggca tcgtctcctc cgacggcgag catctctacc gttatgcggg
      601 tatggggaaa gtcgccgaag ttttcactcc tacgaaattg cgccaactac aaggacggca
      661 tgcaattgca cacaatcgtt attcaaccac cggcgccagc ttcttgcgta acgcgcaacc
      721 gattcgtttt gagtcgcgct tgggcacaat ggcgttggcg cataacggca atttaacaaa
      781 cgctaattcg atacgccatc gattgggaga agagggctcg ctttttcaaa cgactatcga
      841 caccgaggtg attagccacc ttgtcgcccg ctcacgcgcg gataacttta ccgacgcact
      901 tatcgaagcg ttgaaacaaa tccgtggagc gtattcgtta gtcgttttga ccgccgacac
      961 cctctatgcc ttgcgcgacc ctaatggttt tagaccgctg gtgatgggta aaaaagatga
     1021 gggctatatt tttgcctcgg aaacttgcgc tttagacatt atcgacgccg agtatgtgcg
     1081 cgatattgaa ccgggagaat tagtgattgt ttcgccgcgt ggagtaacgt ctctttttcc
     1141 gtttgaaaaa gcgtcgcaca acctttgcat ttttgaatat gtctattttg cgcgccccga
     1201 ttcttatgta tttggtaagt cggtctataa cacgcgccta cggatgggtg aaattttagc
     1261 cgaagagtcc cccgccgatg ccgacttggt gatgcctgtg cctgactcgt cgtcggtggc
     1321 tgcgctcggt tattcacgcc aaagtggcat cccgtaccat atggggctca tacgctcgca
     1381 ctacattggg cgtacgttca ttgagcccga tcaaaaaatt cgtgactttg gtgcaaaaat
     1441 taaatacaac gccatccgct cggttgtcga agggaagcgg gtaatcgtta tcgacgacag
     1501 tattatgcgt ggtacgacta cacgcaaaat tgtaaaaatg ctgcgcgttg ccggcgcaaa
     1561 ggaaatccac attcggattg ctagcgcacc gacaagacat ccctgttatt atgggattga
     1621 tatccccagc cgctcagaac tcatcgcggc gacgcatagc ttagaagaaa tcatcaaata
     1681 cttacgcgtt gattcgttgg catatctttc gctggaaggc atgctacgcg gtgaaaaaga
     1741 gaccgagagt cgaggtaaaa gcggtacgcg tggctattgt accgcctgtt tcgacggtaa
     1801 ctaccctgtg agtttagatg aaaatgaagc gaactactca aaccaaaaaa cgctcttcag
     1861 cgaataccaa gtcgaagaac acacgtaaag gcagcgcttc gactgcgctt agcaacctct
     1921 cttctgtata cgtgcgtcat cctaaaagtc gagtcccgag caaagtcgag gcacgcgtgc
     1981 ttattgttgc tggtgaagaa tcgggggaca tcttgggtgg cgcgattgtg cgcgaagcgc
     2041 tcaagaagaa aaaaattgtg ctgtggggtt gcggtggcga ccgtatggct gctgcaggcg
     2101 ttaaaatcca ttacaccgcc aacgaactgg cgacgattgg tttttgggaa gcggcaaaaa
     2161 attaccgtcg tctcactaaa attgtgaacg cgcttgtgga aaaagctgtc cagaacggga
     2221 cgaaaaaggc ttggctcatc gactttcccg ggtttaattt gcttttggca aaaagactca
     2281 aagcgaaagg aatcgctgca accctcgtcg tttcgcccac cgtctgggcc tggaaataca
     2341 accgggtatt caaaatacgc gaccgctttg aacgcgtgct ctgcctttaa gcttttgaac
     2401 ccgacgtcta taaagaaatt ggcgtcgcag cgc
//
LOCUS       DAOMPD010000414         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17359_length_2424_cov_2.319122, whole genome shotgun sequence.
ACCESSION   DAOMPD010000414 DAOMPD010000000
VERSION     DAOMPD010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17359_length_2424_cov_2.319122"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..815
                     /locus_tag="PLY93_12165"
     CDS             93..815
                     /locus_tag="PLY93_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP70281.1"
                     /translation="MEITEFGDTHNPPAIFLHGYGAHPHLYREFIARLSEKYHVFVPH
                     LFGLNNACRRSFAGNVEAMRDFLEKAQLTSSFVVGHSYGALVAMHLATYYPKLLRAVA
                     VNPLLPNIFHGEKLRRQFENIQKDLQHATGGIRGILTHPEVGIRYGMNILSNPLGYID
                     GATNAIVSALPEAQSEVPVDLVYADLDALFHIDNADVERWRQALPQLQFNPIPDYSHN
                     WIIYYGSYAYDRLQNLFPTESA"
     gene            812..>1285
                     /locus_tag="PLY93_12170"
     CDS             812..>1285
                     /locus_tag="PLY93_12170"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP70282.1"
                     /translation="MNNIFKKIKDTLPSTAEVQGAIQGIIGDKLPGKFATTMDFYADG
                     KRQNTDLIPLQKHVVIFIHGNSDTELGWRKKNTPFNFGDNLMRDFAVTPLYIRYNSGL
                     AVSENGEKLTALLEAFVQKHTKVKSLTLVAHSMGGLITHAAIYHARAQEKIWLTKL"
     assembly_gap    1286..1295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1296..1724
                     /locus_tag="PLY93_12175"
     CDS             <1296..1724
                     /locus_tag="PLY93_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70283.1"
                     /translation="GAPLAKFAEKSEQILQFIPNPVTLMAASVISLRSRGLKDLSHGQ
                     SGLPSENVVLQKNVHYVLIAGGLGQNENGVVNKVVGDGMVRRPSATQKQMPQSSWLST
                     AFPWFKKSTPISVEVLSGVGHLALRTSDEVYAVIQKYFRN"
     gene            1807..>2424
                     /locus_tag="PLY93_12180"
     CDS             1807..>2424
                     /locus_tag="PLY93_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70284.1"
                     /translation="MPTILEDLKDAVVIVTGSGRGIGRGIAECFAAQGAKVVITDIDH
                     ATAQQTAQEIEKMGVETLAMDCDVSKYDQAEALANKVMEKWGKIDVLVNNAGITQDGL
                     FLRMKPEQWQKVLDINLTGTYNCTHAVVNHMRKARKGAIINISSIAAGGNPGQANYSA
                     SKAGVLGFTYALGKELAALGIRVNAVAPGFIKTPMTDKIPEKLREQ"
BASE COUNT          658 a          578 c          595 g          583 t
ORIGIN      
        1 aaacgcactc aacttgaccc caaaagtgtc agttatctct gtgggttgca taaatgtcgt
       61 tttcagtgca aatatatttc agaacttggg ttatggaaat taccgagttc ggtgacacgc
      121 ataatccccc tgcaattttt ttacacggct atggggcgca tccgcattta tatcgtgagt
      181 ttattgcacg gttgtctgaa aaataccacg tctttgtgcc ccatcttttt ggcttaaaca
      241 atgcgtgccg acgcagtttt gcgggtaacg tcgaagcaat gcgcgatttt ttagaaaagg
      301 cacagttgac ttccagtttt gtcgtggggc attcgtacgg tgcgttggtc gcgatgcatt
      361 tagctacgta ctatccaaag cttttgcgtg cggttgcagt caatccgctc ttacccaata
      421 tttttcacgg cgaaaaacta cgccgtcagt ttgaaaatat ccaaaaggat ttgcaacacg
      481 ctaccggcgg catccgtggc atactgactc accccgaggt gggtatccgc tatggtatga
      541 atattttgtc gaatccgttg gggtatatcg acggcgcgac gaacgcgatt gtgagcgctt
      601 tacccgaagc ccaaagcgaa gtgccggtcg atttggtata cgccgattta gacgcacttt
      661 tccatatcga caacgcggat gtagagcgtt ggcggcaggc gttaccgcag ttgcagttta
      721 acccgatacc cgattatagt cataactgga ttatttacta cggctcttat gcgtacgata
      781 gactacaaaa tttatttccc acggaaagcg cttgaacaac atcttcaaaa aaattaaaga
      841 cacgttgcct tcgactgccg aagtgcaagg ggcaatccaa ggcattattg gggataaact
      901 ccctggaaaa ttcgccacga ccatggattt ttacgccgac ggtaaaaggc agaacaccga
      961 tttaattcca ctccagaagc acgttgtaat ttttatccac ggtaactcag acaccgaatt
     1021 aggctggcgt aaaaaaaaca caccgtttaa ctttggggat aatctcatgc gtgattttgc
     1081 ggtcactcca ctttatatac gctacaacag cggccttgcg gtgagcgaaa acggcgaaaa
     1141 gctcaccgcg cttttagagg cgtttgtcca aaagcatact aaagtaaaat cgctcacact
     1201 tgttgcacat agcatgggag ggctcatcac tcatgcggcg atttaccacg cccgcgccca
     1261 agagaaaata tggctaacga aattannnnn nnnnnggggc gcctttggca aagttcgccg
     1321 aaaaaagcga acagatttta caatttatcc ccaatcctgt gacgctcatg gccgcttcgg
     1381 taatttcact acgtagccgt ggactcaaag atttgagcca tgggcaatcg ggcctaccgt
     1441 cggaaaatgt agtgttacaa aaaaacgtac actacgtgtt gattgcaggc ggcttggggc
     1501 agaatgaaaa cggtgtggtc aacaaagtcg taggggatgg catggtgcgg cgacccagcg
     1561 caacacaaaa gcaaatgccg caatccagct ggctttcgac ggcattcccg tggtttaaaa
     1621 aaagtactcc catttctgtc gaggtgctgt cgggggtagg gcacttggcg ttacgcacgt
     1681 cggacgaggt gtacgcggtg attcagaagt attttaggaa ttgatcctag tggccgggga
     1741 ggatggcgca caagctctca gttgctcaaa gtattgtcgt aatgtttcag cgctcaaaaa
     1801 tgacccatgc caactatttt agaagactta aaagacgcgg tggtgattgt taccggttct
     1861 ggtcgtggga ttggccgtgg tatcgcagag tgctttgccg cacaaggcgc taaagtggtg
     1921 atcaccgaca tcgatcacgc aaccgcacaa caaaccgctc aagaaattga aaagatgggt
     1981 gttgaaaccc tcgcgatgga ttgcgacgtc tctaaatacg atcaagccga agcgctcgcc
     2041 aacaaagtca tggagaaatg gggtaaaatt gatgtccttg tcaacaacgc aggcatcacc
     2101 caagacggtc tctttttacg catgaaaccc gaacagtggc aaaaagtcct cgatattaac
     2161 ttaacaggca cttataactg cacgcacgca gtggtaaacc acatgcgcaa ggcgcgcaaa
     2221 ggcgccatca tcaacatcag ctcgattgcc gccggtggca atcccggaca agcaaactac
     2281 agcgcgtcta aagcgggtgt gttgggattt acctatgcac tcggtaaaga actcgccgcg
     2341 ctgggcatcc gcgtcaacgc cgttgctcct gggtttatta aaaccccgat gaccgataaa
     2401 atacccgaaa aattgcgcga acaa
//
LOCUS       DAOMPD010000415         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17560_length_2399_cov_2.528584, whole genome shotgun sequence.
ACCESSION   DAOMPD010000415 DAOMPD010000000
VERSION     DAOMPD010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17560_length_2399_cov_2.528584"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>738)
                     /locus_tag="PLY93_12185"
     CDS             complement(<1..>738)
                     /locus_tag="PLY93_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP70285.1"
                     /translation="SWAKTRARVKRLIDSIAEELIELYALRQQQKGFAYPLDSTFQHD
                     FEAAFPFAETEHQLEAIYAIKRDMESEKAMDRLVCGDVGFGKTEVAIRAAFKAAMAGK
                     QVAILCPTTILAFQHYRSFVKRFSDYPVTIDFLSRFRTTTESQDIKKKIAEGKIDIII
                     GTHSLLSNDVKYKSLGLLIIDEEQRFGVQHKEKLRQMKHNLDSLALTATPIPRTLHMS
                     LAGIRDLSIIETPPVDRRKIETHVLAE"
     assembly_gap    739..758
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<759..2346)
                     /locus_tag="PLY93_12190"
     CDS             complement(<759..2346)
                     /locus_tag="PLY93_12190"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRP70286.1"
                     /translation="MTFAFQDTVQELRSGILNAALPLSALPLTAYLHYQKYPERVLYI
                     VPDDNTALTIHRTLDALQKIHNTNFNTGALVSWGVIAYSYAAADKEKEYLRTQAERLI
                     LNNSPCLLIASVEGLTLPIAVDGSAAQSLALKAGMRLERETLVQFLVDAGYTYAEPPP
                     VTKPGEFCVKGGIVDVFPPNLDYAVRIDYFGDDIESIKAFSHETQRSFSTLKDVSLSA
                     LKPNLNFLKKELTTLSQKIQKEKLELPPIVSTEGGNPTGYVDIYPALRRTVFLPELWL
                     LQPGTHAFVADAQSALRRAKILSDERAYLYEKSEGKFRVEPTILFLTQEKVEEFFLQT
                     VAIPVEATEKIPTRFAEVKQYGGRLGALKETINENIDKPFYFFIESESQCERLINALQ
                     DILIPKIFPYYYPHGFKHESFFFLTENDVFGRVVKKYAGDKKISRILDSYTDLKEGDY
                     IVHVNYGIGKFARLKRMKVSGAQRTGKDGPIGERVVVERDFLELIFAEGDKLFVPLDQ
                     LHLVHKYIGSTENPHLDYLGK"
BASE COUNT          599 a          568 c          546 g          666 t
ORIGIN      
        1 ttttcggcga ggacgtgcgt ttcaattttg cgccgatcga cgggcggtgt ttcgataatc
       61 gaaagatcgc ggatgccagc cagcgacatg tgtagcgttc tggggatggg cgttgcggtg
      121 agcgccaacg aatctaaatt gtgtttcatc tgccgcaatt tttctttatg ctgcactccg
      181 aagcgttgtt cttcatcgat gatcaaaagt cccaacgatt tatacttcac atcgttcgac
      241 aaaagagaat gtgtaccaat aataatatcg attttccctt cggcgatttt tttcttaata
      301 tcttgacttt ctgtagttgt gcggaatcga cttaaaaaat caatcgtcac tggataatcg
      361 gaaaagcgtt tcacaaaact gcggtagtgt tggaatgcca aaatagtcgt ggggcataaa
      421 atcgccacct gctttcctgc catcgccgcc ttgaacgccg cacgaatagc gacctcagtt
      481 ttaccaaaac ccacgtcgcc gcacacgagg cgatccattg ccttctcgct ttccatgtcg
      541 cgcttgatag cgtaaatcgc ttcgagctgg tgttcggtct ctgcaaaagg aaacgccgct
      601 tcaaagtcgt gttggaacgt gctgtcgagg ggatatgcaa aacctttttg ctgttgtcgt
      661 aacgcgtaga gttcaatcaa ctcctcggcg atagagtcga tgagacgttt tacacgcgca
      721 cgggttttag cccacgacnn nnnnnnnnnn nnnnnnnntt tttcccaaat aatccaaatg
      781 cggattttct gtcgagccga tatatttatg caccaagtgc aattgatcga gcggcacaaa
      841 aagtttatcg ccctcggcaa aaataagttc taagaaatcg cgttcgacaa cgacacgctc
      901 accgatagga ccatctttcc ccgtgcgctg cgctcccgac accttcatcc gctttaagcg
      961 cgcaaattta ccgatgccgt aattgacgtg aacgatgtag tcgccttctt ttaagtcggt
     1021 atagctatcg aggatgcggc taattttttt atcgcctgcg tattttttga cgacacgccc
     1081 aaagacatcg ttttcggtta aaaagaaaaa cgattcgtgc ttaaaaccat gcggatagta
     1141 atacggaaaa attttaggta ttagaatatc ttggagcgcg ttaattaaac gctcacattg
     1201 gctttccgac tcgataaaaa aataaaatgg cttatcgatg ttttcgttta tcgtttcttt
     1261 gagcgctccc aaacggccac cgtactgctt cacttccgca aagcgcgttg ggattttctc
     1321 tgtcgcttcc accggaatag ccacagtttg taaaaaaaac tcttctactt tttcttgtgt
     1381 caaaaaaagt atagttggtt cgacgcgaaa cttaccttcg ctcttttcat aaaggtacgc
     1441 cctctcgtcg ctcaggattt ttgcccgccg caatgcactt tgcgcgtccg cgacaaaggc
     1501 atgcgttcct ggttgaagca accagagctc gggcaggaat accgtgcgac gcagcgcagg
     1561 ataaatatcc acataacccg ttgggtttcc cccttcggtc gatacaatcg ggggaagttc
     1621 taatttctct ttttgaattt tttgactcag cgtcgttaat tcttttttca aaaaatttaa
     1681 attgggttta agcgcagaca gcgacacgtc tttgagagtg gaaaaactgc gctgtgtttc
     1741 gtgagagaat gctttgatac tttcaatgtc gtcgccaaaa taatctatcc gcaccgcgta
     1801 atctaaatta ggcggaaaaa catccacgat accgccttta acacaaaact ctcccggctt
     1861 tgtcaccggt ggaggctcgg cgtaagtgta acctgcgtcg accaggaatt gaacgagtgt
     1921 ttcccgctca agacgcatcc ctgctttgag agcaaggctt tgcgccgcgc taccgtccac
     1981 cgcaatggga agcgtcaacc cttccaccga tgcgatgaga aggcacgggc tgttattcaa
     2041 aatgagtctt tcggcttggg tacgtaagta ctctttttcc ttgtcggcgg ccgcatagct
     2101 atacgcaatc acaccccacg aaactaatgc accggtatta aaattagtat tatgaatttt
     2161 ttgtagcgca tcgagagtcc ggtggatcgt caatgcggtg ttatcgtcgg ggacgatata
     2221 caaaacgcgc tcgggatatt tttggtaatg gagatacgca gtaagcggta aggcggagag
     2281 tgggagcgca gcgtttaaaa tgccactgcg caactcttgc acggtatctt gaaaggcaaa
     2341 agtcaattcc gagggtaatg cgatttcgtc gttggtttat ttcacaccat tttcctttt
//
LOCUS       DAOMPD010000416         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17588_length_2395_cov_2.725641, whole genome shotgun sequence.
ACCESSION   DAOMPD010000416 DAOMPD010000000
VERSION     DAOMPD010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17588_length_2395_cov_2.725641"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLY93_12195"
     CDS             complement(<1..276)
                     /locus_tag="PLY93_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRP70287.1"
                     /translation="MKKRNRLSEILVEKGVIRLAGYTAVVVLLLILFFMFREGTPFLE
                     TYSISSFLFKNEWFPLSDRYGMLPLITGSAMVTLVALSFSIPLSFATA"
     gene            complement(280..1155)
                     /locus_tag="PLY93_12200"
     CDS             complement(280..1155)
                     /locus_tag="PLY93_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRP70288.1"
                     /translation="MKKLTATIGLLFLALLACSRKNNTLKIAGSDTMVQIGQHLAKAY
                     MQQDPQKLISIQGGGSGTGIAALLNGSTDIASASREMKPEELMRAQQRGITVKEYVIA
                     NDALVIAVHPSNKISKLTIEQLSGIYAGKITNWSALGGDNKPILAISRDSNSGTHAYF
                     KDEVLRQGKTVNKGEKSTDPLARLEFGKNVTFAVSSQAISDQVKVNTAAIGYLGIGWA
                     AEGVKSVTISRDGKAFYAPTLENTVQGKYPLARGLQMYVAVKEDQKAIEFMNFVLSPE
                     GQKIVQEQGFIPVKK"
     gene            1280..1636
                     /locus_tag="PLY93_12205"
     CDS             1280..1636
                     /locus_tag="PLY93_12205"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRP70289.1"
                     /translation="MQLREYERMFKALSDKQRLRILALLSRRALVVNDIKLMLRLSMP
                     TISQHLSILRDSDLVLDNKQGRWVEYCIHPKLYDNNNLGGIIYAELKTFFDGDETLNL
                     DFDFLDKINSRMGQVE"
     gene            1720..>2395
                     /locus_tag="PLY93_12210"
     CDS             1720..>2395
                     /locus_tag="PLY93_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRP70290.1"
                     /translation="MITTRTLYHSIALAFFVLLVSEARSSERNAGTPTTEAIEKNKEE
                     TKESNSLKPDFVSYTDDGITFSAGDYSLSMLHRLQFRYAGPFDADPRSVADLKQNTQS
                     FMVRRARLKLAGHAYKPWIKFYIQYDWSQPVLRDFHITLDRFAWLTLRIGRGKVIYND
                     ERVSSSGYQQFVNRSILNDLFTVDRQQGIQLLGRFFENTAADFNYVLGIFTGRDVGER
                     LNDDEIL"
BASE COUNT          614 a          528 c          572 g          681 t
ORIGIN      
        1 agcggtggcg aaagaaagtg ggatagaaaa ggagagtgcc accaaagtga ccatggcgct
       61 gccggtgatg agcggtagca tgccatagcg atccgagagg gggaaccact cgttcttgaa
      121 aagaaaactg gagatggaat acgtttctaa aaaaggagtg ccttcgcgga acatgaaaaa
      181 gagaattaaa aggagaacaa cgactgccgt gtaaccggcg agtcttatga ctcctttttc
      241 tacaaggatt tcgctaagtc gattgcgctt tttcataacc tatttcttga ccggtataaa
      301 gccctgctct tgaacaattt tttgcccttc aggactgagt acaaaattca taaactcaat
      361 ggccttttgg tcttccttta cagcgacgta catttgtaat ccgcgggcaa ggggatactt
      421 tccttgcact gtattttcca aagtgggtgc gtaaaacgcc ttgccgtctc ggctaattgt
      481 aacagattta accccttccg ccgcccagcc gatgcccaag tagccaatgg cggccgtatt
      541 gactttaact tgatcgctaa tcgcttgtga agacacggcg aaagtaacat ttttaccaaa
      601 ttctaaacgg gcgagtgggt cggtggattt ctctccttta tttaccgtct tcccttgacg
      661 cagcacttcg tctttgaaat aagcgtgtgt acccgagttg ctgtcgcggg agattgcaag
      721 aatgggtttg ttgtcaccgc ccaacgcgct ccagttggta attttaccgg cgtaaatacc
      781 gctcagttgc tcgatagtga gttttgaaat tttgttcgag ggatggacgg caatgactaa
      841 cgcgtcattt gcaataacgt attctttgac tgtaatcccg cgttgttgcg cccgcatcaa
      901 ttcttccggt ttcatttccc gcgatgcact ggcaatgtcg gttgagccat taaggagtgc
      961 cgcgataccg gtgccgcttc caccgccttg gatggaaatg agcttttggg ggtcttgttg
     1021 catgtatgct ttagcaaggt gctgcccaat ttgtaccatg gtgtcggagc cggcaatttt
     1081 gagtgtgttg tttttgcggc tacacgcaag gagcgcgagg aagagtagtc ctatcgttgc
     1141 tgtaagtttt ttcattgcgg tcacgaaaat taggttagtc tatttgtata tccacattta
     1201 ggtaaatagc gaaatatcta tacgttgaga gctgtgtgca ttgtttgaca cggtacttaa
     1261 cttagacaat tcgttaatta tgcaattacg cgaatacgaa cgtatgttta aggctctttc
     1321 cgacaaacaa cgcctgcgta ttcttgcgct actctcgcgc cgtgctctgg tagtgaatga
     1381 tataaaactc atgctccgcc tctctatgcc tacaatttca caacaccttt cgattctgcg
     1441 cgattcggat ttagtgttag ataataaaca aggacgctgg gtggagtact gtatccatcc
     1501 taagctttac gataacaata acttgggggg gattatctac gcagagctca agacattttt
     1561 tgatggcgat gaaactctca atttagattt tgatttttta gataaaatca atagtcgaat
     1621 ggggcaagta gaataatatg tttatatttc gctaaataac aaaatgttgt atacgtatta
     1681 ttctcccttt aggaaatgta acctcagtag gaaaaataaa tgataacaac aagaacactc
     1741 taccatagca ttgcgcttgc cttcttcgtg cttttagttt cagaagcgcg ttccagcgaa
     1801 cgcaacgccg gtacgccaac gacagaggcg atagaaaaga ataaagaaga aacaaaagag
     1861 agtaactcgc tcaagccgga ctttgtttct tataccgatg acggcatcac ctttagcgcc
     1921 ggtgattact cgctttccat gctgcaccgc ttgcagttta ggtatgcagg ccccttcgat
     1981 gccgacccgc gtagcgtggc ggatctaaaa cagaacacgc aatctttcat ggtgcgtcgt
     2041 gcgcggttaa agctggcagg ccacgcgtat aaaccgtgga ttaaatttta tatccaatac
     2101 gattggtcgc agccggtctt gcgtgatttt catattacgt tagaccgctt cgcttggctg
     2161 acgttgcgca ttggtcgcgg taaagtgatc tacaacgacg aacgggtctc gtcatcgggg
     2221 tatcaacagt ttgttaaccg ctcaatcctg aacgaccttt ttaccgtcga tcgtcagcaa
     2281 ggtatacaac ttctggggcg tttctttgaa aataccgccg ccgactttaa ttatgtgctt
     2341 ggtattttta ccggacgcga tgtgggggag cgtttgaacg acgacgaaat cctca
//
LOCUS       DAOMPD010000417         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17631_length_2391_cov_2.815497, whole genome shotgun sequence.
ACCESSION   DAOMPD010000417 DAOMPD010000000
VERSION     DAOMPD010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17631_length_2391_cov_2.815497"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLY93_12215"
     CDS             <1..445
                     /locus_tag="PLY93_12215"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803771.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRP70291.1"
                     /translation="QGALTYILQSADGQVHDVHSVSAGLDYPSVGPEHAYLKEQGRAQ
                     YVAIKDKEALAAFQELSKQEGIIPALESAHAVAAAKNYVEALHKKGKKNLSILICLSG
                     RGDKDVQEVERLLAIPEALEHEESEPPPASFTTAPTVKVVATGGI"
     gene            455..>763
                     /locus_tag="PLY93_12220"
     CDS             455..>763
                     /locus_tag="PLY93_12220"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRP70292.1"
                     /translation="MRVGLGYDIHRTQSGSSIVLGGVTFDAPFSLVGHSDADALLHAI
                     TDAILGALADYDIGYYFPPSKEENKKRASSDFLFFAVERMRTRGYAIENIDCNVICEV
                     P"
     assembly_gap    764..773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <774..956
                     /locus_tag="PLY93_12225"
     CDS             <774..956
                     /locus_tag="PLY93_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRP70293.1"
                     /translation="IQPVREKIRQNLAKLLSVDIERVSVKGRTKEKVDAVGRGEALEC
                     QAVVLLSAKAGDNAVE"
     gene            complement(924..1883)
                     /locus_tag="PLY93_12230"
     CDS             complement(924..1883)
                     /locus_tag="PLY93_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70294.1"
                     /translation="MADALDKRPTHAPRYRREITILSLAFLFSGVILLGVFIHGVAFF
                     LWAFLFALMLCGAIFIVFTASRWTPLKRAVLFYLVLLLSIRAASEYLFAFFIFTALFY
                     TLFSLLWKDSHTQRHSELPGVLAALLLCFTLYGVFSSVVESIRSAYLKIEKSISLQCE
                     EDKHERICKDSASIFSIPEFWKHGGFSSLVIDLQKIMPLQNFIDSATQNTIAFAALDL
                     PMEQVVQEMQKFFSSQKSFLTSKAAKGEPLLPKLIMKNKDSMLMALNYESVKMPLYLS
                     ERDESAALLLLLKLRQSTWLFIIDGKAVEGREFLLNRIVTGFR"
     gene            complement(1932..>2391)
                     /locus_tag="PLY93_12235"
     CDS             complement(1932..>2391)
                     /locus_tag="PLY93_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRP70295.1"
                     /translation="LSVAMGSDVNLVNAPILAKERQLKITGKKDPQGENETPSLSIRV
                     VDEKETMTLKATVGTVGGFITELNGNSIELHPEGNLLYIENTDTPGVVGHLGTFLGAH
                     KINISSMQLSRAQKGGAARTFLGLDDEMSKELIDELKKKEFIVEARFIKI"
BASE COUNT          672 a          570 c          569 g          570 t
ORIGIN      
        1 gcaaggcgcg ctgacgtaca ttctacagag cgctgacggc caggtccacg atgtgcattc
       61 agtgtcggcg ggactcgact acccgagcgt tggacccgag cacgcgtatc tcaaagaaca
      121 aggcagagcg caatacgttg cgattaaaga taaagaagcg ctcgccgcat tccaagaact
      181 ctccaaacaa gaaggcatta ttcctgcgct cgagtcggcg catgcggtag cggctgctaa
      241 aaattatgtc gaggcgttac acaagaaagg taaaaaaaat ctatcgattc ttatttgtct
      301 ttcggggcgc ggcgacaaag atgttcaaga agtcgaacgc ctgctagcaa tcccagaagc
      361 tcttgaacat gaagaaagcg aaccgccacc agcctctttt acaacggcac ccacggtgaa
      421 agtcgtcgcc actggcggca tctaaattaa acgcatgcgc gttggtttag gttacgatat
      481 ccaccgtaca caaagcggca gttctattgt tttaggtgga gtgaccttcg acgcgccttt
      541 ttcgctcgtg gggcattccg atgccgacgc gctcttgcat gccatcaccg acgcaatttt
      601 aggcgcgctc gccgattacg acattgggta ttatttcccc ccctcgaaag aagaaaataa
      661 aaaacgcgcg agtagtgatt tcttattttt tgcggtggag cgtatgcgta cgcgtggcta
      721 cgctattgaa aacattgatt gtaacgtcat ctgcgaagta cccnnnnnnn nnnattcaac
      781 ccgtccgaga aaaaatccgg caaaacttgg ccaagctctt gtcggtagat atcgaaagag
      841 ttagcgtgaa agggcgtacg aaagaaaaag tcgatgcggt ggggcgcggc gaagctctcg
      901 aatgccaagc ggtagtgctc ttgtcagcga aagccggtga caatgcggtt gagtaaaaac
      961 tcgcgtcctt cgactgcttt tccatcgatg ataaaaagcc aagtggattg cctaagtttg
     1021 agcaacaata gcagcgctgc ggattcgtct ctctcgctga gatacaaagg catttttaca
     1081 ctttcgtaat tcaacgccat aagcatcgaa tctttatttt tcatgataag tttgggcagc
     1141 aatggttcac cctttgcggc tttactcgtt aaaaaacttt tttgcgagga gaaaaatttt
     1201 tgcatctctt gcacgacttg ctccatcggt aaatctaacg cggcgaacgc gatggtgttt
     1261 tgagtagcgc tgtcgataaa attttgaagc ggcataattt tttgaagatc gataactaac
     1321 gaagaaaaac caccgtgctt ccaaaattca ggtatagaga aaatagacgc gctatccttg
     1381 cagatccgct cgtgtttgtc ttcttcgcac tgaagggaga tgcttttttc aatttttaaa
     1441 tatgcactgc ggatagattc cacgacgctc gaaaaaactc cataaagcgt gaaacataaa
     1501 agaagcgctg ccagcacccc tggtaattct gagtgtcgtt gcgtgtggga atctttccat
     1561 agtaacgaaa aaagcgtgta aaaaagtgcg gtaaaaataa aaaatgcaaa aagatattca
     1621 ctcgccgcgc gtatcgacaa caggagcaca agataaaaaa gcactgcacg cttcaatggt
     1681 gtccaacgcg atgcggtaaa aacaataaaa attgcaccgc acagcataag cgcaaaaaga
     1741 aacgcccaaa gaaaaaacgc cacgccgtga ataaacacgc ccaaaagaat aacgccacta
     1801 aatagaaacg ctaacgatag aatggtgatt tcacggcggt aacgcggcgc gtgcgtcgga
     1861 cgcttatcta aagcgtcagc catgcgccgc aaaaaacgac taactataga cggcacgccg
     1921 ctcacaggcg actaaatttt aataaagcgt gcctcgacga taaattcttt ctttttgagt
     1981 tcgtcgatga gctctttcga catttcatcg tcgagtccca agaaagtccg cgctgcaccg
     2041 cccttttgag cacgcgagag ctgcatgctg gagatattga ttttgtgcgc acccaagaag
     2101 gtacccaagt gacccacaac gcccggggtg tctgtgtttt caatataaag caaattgcct
     2161 tcggggtgca gctcaatcga gttgccattg agttcggtga tgaatccgcc gacggtgccc
     2221 accgtggctt tgagcgtcat cgtttctttt tcgtcgacaa cgcgtatcga aagcgaaggc
     2281 gtttcgtttt caccttgcgg atcttttttg ccggtaattt taagctgacg ctctttggct
     2341 aaaatgggtg cattcactaa attcacgtcg ctccccatcg ccacagaaag c
//
LOCUS       DAOMPD010000418         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17733_length_2379_cov_2.556368, whole genome shotgun sequence.
ACCESSION   DAOMPD010000418 DAOMPD010000000
VERSION     DAOMPD010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17733_length_2379_cov_2.556368"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLY93_12240"
     CDS             complement(<1..271)
                     /locus_tag="PLY93_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRP70296.1"
                     /translation="MRRAYYEIRRMLAVNSIFIDLLIDVFIGYLYRPRGLRPRSFSRR
                     LLAGTVRRIFRRPKNFARFPVQFRRTLERLGPTYVKLGQILSLRDD"
     gene            413..>2379
                     /locus_tag="PLY93_12245"
     CDS             413..>2379
                     /locus_tag="PLY93_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP70297.1"
                     /translation="MLRDRTIQYLLLSGFSALMLNCSGKTYSTFFQPFDLLFNRSSTS
                     LSMVSATPLTNTRVMLTYSKAIDFDSAVNPANYTVKGPDGNLLHIMAVSRDPNNANVI
                     FVDTAPQSAGKEYTVTASNIKGVDGTPLGGSNSAKFTAPNNADQMGPQFSQVTAVNGT
                     TLEVYFNEAMDKTSAETAANYDIYTDAACSSGNVNVTGAVRDTSNIAKVTLISASMTA
                     GTTYYLCATNAVKDMWGNANAATIASQPFVYSAPTPKVSSAVSSSPTSLVVTFDQKMA
                     STGLTTAGNYTFANCGALTNATGATITALNNNTQVLLTGLTVGTTGTCKVTVNTAIQS
                     AAGSALTAATNSALFAYNTADTTGPQIVSMAPTNSNTVEIIFTENIDVSSITSGTIAF
                     SPTLNVTGVVCIGNVCTVTTGDQSTTTYTATVTGVKDGVGNTTGSTSTTFTGDGKPYI
                     VAIYPVDQNTVMVEWSEPIGNAASVDAGDYSISGVTITGAALYPSGVDPSRYVQLTIN
                     PALTAGTGYTLSVNNPTGSTDSTGNGTLGTVPNGGAFTGPTSTQAPLITSASSPSPTT
                     VLVNFNEPLFNPITAGAFSFTGVGCALGTPTAATEIQAGVILLTIPGQAADAAACKVE
                     VATANQVKDLAGNGNAAGLTHANAFAYTGTGS"
BASE COUNT          610 a          601 c          636 g          532 t
ORIGIN      
        1 tatcgtcgcg caacgataaa atttgtccca gcttgacata agtgggaccc aagcgttcga
       61 gtgtacggcg gaactgcact gggaatcgcg caaaattttt agggcggcga aaaattcgac
      121 ggacggtgcc tgcgagtagg cggcgcgaaa aagaacgcgg gcgcaaccct cgggggcggt
      181 ataaataacc aatgaacacg tcaataagca ggtcaataaa aatcgaattg acggcaagca
      241 ttcgacggat ttcgtagtag gcgcggcgca tttggtttga gatagacgcg ttgaattacg
      301 tgtcaacttt taattgaatt gggaatatcg tcaaattcat gaaatctata gatttctcct
      361 caaaaaaaat aaaaaatgtc gtaaaacaaa caatttttcc gtcaaaatat gaatgcttcg
      421 cgatagaact atccaatatc ttctcctgtc tggtttttca gccctcatgc tcaactgttc
      481 ggggaaaacg tacagtacgt ttttccagcc attcgattta ctcttcaatc gctcgtcgac
      541 atcgctgtcg atggtttcag ccacgccact caccaacacg cgggtgatgc tcacgtattc
      601 aaaggcgatt gattttgact cggcggtgaa tcctgcgaat tatacggtta aaggccccga
      661 cggcaatttg ttgcacatca tggcggtgag tcgcgatccc aacaacgcga acgtaatttt
      721 cgtcgatacg gcgccgcaga gcgcagggaa agaatacacg gtgactgcgt cgaacatcaa
      781 aggagtggat ggtacgccgt tggggggctc gaacagcgcg aaatttactg cgccgaataa
      841 cgccgaccag atggggccac agtttagtca agtaacagcg gtgaatggca ctacgctgga
      901 agtttatttt aatgaagcta tggataagac aagcgcagag acagctgcga actatgatat
      961 ttatacggat gcggcatgct cgtcgggcaa cgtcaacgtg acgggcgccg tgcgtgatac
     1021 gtcgaatata gcgaaagtga cgctcatcag cgcatcgatg acagcaggaa cgacatatta
     1081 tctttgtgca acgaatgctg taaaagatat gtggggcaat gcaaatgcgg cgacgattgc
     1141 ttcgcaaccg tttgtctata gcgcgccgac gcccaaagtt tcgagtgctg tgtcttcatc
     1201 acccacgtcg ctcgtggtga cgttcgacca aaagatggcg tcgacgggac taaccaccgc
     1261 cggcaattat acgtttgcaa attgcggggc tctcaccaat gctaccggcg cgacaattac
     1321 cgcactcaat aacaatactc aagtgctgtt gaccggcctc acggtgggca cgacgggtac
     1381 gtgtaaggtt acggtgaata cggccattca gtcggctgcg ggctccgcgc taacggccgc
     1441 gacaaactcg gcgctctttg cctataacac agcggacacc accggcccgc agattgtgag
     1501 catggcgccc accaatagca ataccgttga aattattttt acagaaaata tcgatgtcag
     1561 ttccatcacg agcggaacga ttgccttctc tcccacactc aacgtgacgg gggttgtttg
     1621 tattggcaat gtctgcactg ttacaacggg cgatcaaagt acaacgacgt acactgcaac
     1681 cgtaacgggc gttaaagatg gggtaggcaa caccaccggc tcaaccagta cgacattcac
     1741 cggtgacggt aaaccgtaca tcgttgcgat ttatcccgtc gatcagaaca cagtcatggt
     1801 cgagtggtcg gaacccatcg gcaacgcggc gtcggtcgat gcgggggatt actctatttc
     1861 gggtgtaacg atcaccggcg cagcgcttta tccctcgggc gtcgacccgt cgcgttatgt
     1921 gcagttaacg ataaatccgg ctttgaccgc cggtacgggc tatacgttga gcgtcaataa
     1981 ccccacgggt agcaccgact ctacgggcaa tggtacattg ggtactgtgc ctaatggcgg
     2041 cgcatttacg gggccgactt cgacgcaggc gccactgatt acaagcgcgt cgtcgccctc
     2101 gcctacgacg gtgctggtga atttcaacga accgctgttt aatccgatta cagcaggagc
     2161 gtttagtttt acgggcgtag gctgcgcttt aggaacaccc accgcggcga cagaaattca
     2221 agcaggcgtc attcttttga cgatacccgg tcaagccgcc gatgcggccg cttgtaaagt
     2281 agaagttgct acagcaaatc aggtcaaaga tctggcgggt aacggtaacg cggcaggttt
     2341 gacgcacgcg aacgccttcg cttataccgg cacgggttc
//
LOCUS       DAOMPD010000419         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17850_length_2367_cov_2.233564, whole genome shotgun sequence.
ACCESSION   DAOMPD010000419 DAOMPD010000000
VERSION     DAOMPD010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17850_length_2367_cov_2.233564"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLY93_12250"
     CDS             <1..341
                     /locus_tag="PLY93_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRP70298.1"
                     /translation="TKQGADEAVTIPRGNTASTTRWKKGGARRLVSLPTIDYPASIRK
                     RSGQGSVELLLVVGATGRVESVEVIKSSGYSKLDINAKNAYRNAVFSASPSGEKASGV
                     VVVTFRMRDN"
     gene            341..1096
                     /locus_tag="PLY93_12255"
     CDS             341..1096
                     /locus_tag="PLY93_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP70299.1"
                     /translation="MSAVELKNLSLRIGTKQILSNIDVSINPGETLVLMGKNGSGKTM
                     LLKTLVGLFKPQEGTASIFSQSVHAMSHHELDRLRQKIGYVFQKSGLFDSLHVWENVI
                     FALRRFHNLSEAELRDKATECLNRSGLKEVEDKYPSELSGGMQKRAGIARAIAMDPQL
                     LLLDDPTAGLDPVLTDAIADLIIDIQKRLGSTAIIVAHDFKLAYKLADRIGLMVGGRL
                     HGVVSRDEFKTTTEPYFEQFREGKLVGPIPVIE"
     gene            1098..>2367
                     /gene="guaD"
                     /locus_tag="PLY93_12260"
     CDS             1098..>2367
                     /gene="guaD"
                     /locus_tag="PLY93_12260"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HRP70300.1"
                     /translation="MPQKKKGICAAVINPVAENQIEYFDKAYLLWDEQGTLTYFSDKK
                     PDGGTWDSYPIEHQNDKIAIPGLIDLHTHLPQYEFVAQGAEALLPWLEKYTFPQEARF
                     ADNAVAREQSHNFFQSCVANGTTTTVAYLSSFFDAAHVAFEEASRVRLRAYLGLTLMD
                     MNVPRALQTKTSIAEKNMRTLIEKFHKKGLNEFVVTPRFAVSCSEEMLRMCGAVSREY
                     DTFLQTHISENSAEVREVAALFPQYKNYTDVYRQTGCLHAKTLLGHGIHLTHEERKII
                     AETKSVVVHCPTSNTFLGSGIFSYGVLLNENLRLGLGTDVAGGYSLSMFHEAKQAVEM
                     AKLRAHFDATPSALNSGSALYQATLGNAHALGRADELGSFAVGKQADIALIDDARTNP
                     LLENAKNHYASLAERLNRVLYRGHPGMVAQT"
BASE COUNT          630 a          587 c          617 g          533 t
ORIGIN      
        1 cgacaaagca aggcgccgat gaagcggtga cgataccacg cggcaacacg gcgtcaacaa
       61 cccgctggaa aaaaggcgga gcgcggcggc tcgttagttt acccacgatt gattaccccg
      121 cgagtatccg aaagagatcg ggacaaggga gcgttgagct tttactcgtt gtcggcgcga
      181 cgggacgtgt agaaagcgtc gaggtgatta aatcgtcggg gtatagcaag ctggatatca
      241 acgcaaagaa tgcgtaccgc aacgcagtat tctcagcgtc gccttcgggt gaaaaagcgt
      301 cgggtgttgt cgtggtgacg ttccgcatga gggataacta atgagcgcgg ttgaattaaa
      361 aaatttatcg ctgcgcatcg gcacaaaaca aattctatct aatatcgatg tgagcatcaa
      421 cccaggcgag acgttggtgc tcatgggaaa aaatggttcg ggtaaaacga tgctcttgaa
      481 aactctcgtg gggttattca aaccacaaga agggactgcc tccattttct ctcagagcgt
      541 gcatgcgatg tcgcaccacg agttagatag actgcggcag aaaatcggtt atgtcttcca
      601 gaaatcggga ctcttcgact cgctccacgt ttgggaaaat gttatttttg cactgcgccg
      661 ttttcacaat ttaagcgaag cggaacttag agataaagcc accgagtgcc tcaaccgctc
      721 tggtcttaaa gaggtcgaag ataaataccc ctccgaactt tcgggtggga tgcaaaagcg
      781 tgcgggtatt gcgcgcgcga ttgcgatgga tccgcaactt ctacttttgg atgacccaac
      841 ggcggggtta gaccccgtac tcaccgacgc catcgccgat ttaattatcg acattcaaaa
      901 aagattaggc agtacagcga ttatcgtcgc gcacgatttt aagctcgcgt ataaactcgc
      961 cgaccgcatc ggcctcatgg tcggtgggcg tttgcacggc gtcgtctccc gcgatgaatt
     1021 taagacaacc accgagccct actttgaaca atttcgcgaa ggaaagttag tggggccaat
     1081 ccccgtcatt gaataacatg ccacagaaaa agaaaggaat ctgcgctgcg gtgataaacc
     1141 ctgttgcaga aaatcagatt gagtattttg ataaagcgta tctgctttgg gacgagcagg
     1201 gcactctcac atatttcagc gataaaaagc cagacggggg tacttgggat agttatccta
     1261 ttgaacacca aaacgataaa attgccatcc ccggtctcat cgatttgcac acgcacttgc
     1321 cgcaatatga atttgtcgcg caaggggcag aggcgctttt accatggctc gaaaaatata
     1381 ccttcccgca agaggcgcgc tttgccgaca acgctgtcgc gcgggagcaa agccacaatt
     1441 ttttccaaag ctgcgtggcg aacggtacca ccaccaccgt cgcatacctc tcttcttttt
     1501 tcgacgccgc tcacgtggca tttgaagaag catcgcgtgt gcgcttgcgc gcgtatttgg
     1561 gattaacgct catggatatg aatgtgcctc gtgcgttgca gaccaaaaca agcattgccg
     1621 aaaagaatat gcgtacgctc attgagaaat tccacaagaa aggcctcaat gaatttgtgg
     1681 tcaccccccg ctttgccgtc agttgcagtg aagaaatgct gcggatgtgc ggcgcggtga
     1741 gtcgagagta cgacacgttt ttgcaaacgc atatcagcga gaactccgcc gaggttagag
     1801 aagtggcggc gttatttcca cagtataaaa attacaccga tgtgtaccgc caaaccggtt
     1861 gcctgcacgc gaagacgttg ctcggccacg ggattcattt aacacacgaa gagagaaaaa
     1921 taattgcaga aactaaaagc gtcgtcgtgc attgccccac ctcaaatact tttttgggaa
     1981 gcggcatttt ttcgtatggc gtattgctga atgaaaatct gcgtttgggt ttgggcaccg
     2041 acgttgcggg tggatactcg ctatcgatgt ttcacgaagc taaacaagcc gtcgaaatgg
     2101 caaaactccg cgcgcacttt gacgcaacgc cttctgcact caacagtgga agtgcgctct
     2161 accaagcgac actggggaat gcacacgcgt tgggccgcgc agatgagtta gggagctttg
     2221 ccgtggggaa acaagccgac attgcgctca ttgacgatgc acgcacaaac ccacttttag
     2281 agaacgcaaa aaatcactac gcttcgctcg ccgaacgctt aaaccgtgtt ctctaccgtg
     2341 gccatccagg aatggtcgcc caaacat
//
LOCUS       DAOMPD010000420         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17918_length_2359_cov_3.125868, whole genome shotgun sequence.
ACCESSION   DAOMPD010000420 DAOMPD010000000
VERSION     DAOMPD010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17918_length_2359_cov_3.125868"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLY93_12265"
     CDS             <1..656
                     /locus_tag="PLY93_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70301.1"
                     /translation="TCHPYCRHRLSPVPEETLVDVVSASPETHSEIEKRIQKQTSGNI
                     DISRLDTVGQRSLSNVLEDIYERYPNLPKLESVVATNRHLSDENGAPIFASMDNKELK
                     IYAGAQDNDNLRRILKRENRDLKAINGRTAHHYESIDDVIRHEFGHYIAESNNMIGGN
                     YTIFTGMVENAGLMSHIMTNKNEFFPELFLLFEKGVLAEKLPIVDAHMRQLLTRIFG"
     gene            653..856
                     /locus_tag="PLY93_12270"
     CDS             653..856
                     /locus_tag="PLY93_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70302.1"
                     /translation="MTKGVSQCLYCTHYQSIRHGEKIPRCKAFPDGIPMPLFFTEVSH
                     RKPYPLDNGIQFEPIDELKESEE"
     gene            856..1281
                     /locus_tag="PLY93_12275"
     CDS             856..1281
                     /locus_tag="PLY93_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70303.1"
                     /translation="MILTYYLDSSLNPVPTGNAFGRASKVHTLLNWHPRWQYLSPNEM
                     RRKIVTASAQLDNLSYKGSKADPAQPMQFPRSMGQDPAFSLDEQIRRLLSALVQQVEY
                     NLNRAGMGMPQYGHGNEQFTARQDTISREALQTLYPYLA"
     gene            1406..1582
                     /locus_tag="PLY93_12280"
     CDS             1406..1582
                     /locus_tag="PLY93_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70304.1"
                     /translation="MDDVAEADLVSATQLEIGDLIFVQRGWQRNDIYRVMTVSPSSMG
                     RHQYKLTRKLDDAA"
     gene            1635..1949
                     /locus_tag="PLY93_12285"
     CDS             1635..1949
                     /locus_tag="PLY93_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70305.1"
                     /translation="MAFHFGDTLEKGEVDEVLKNAPVARWLVPVANDKVDELACSTEG
                     LIQTFRASLVIVAKTASNCTQELIEVLQKVYTTSEDVAGVHDSPSSPHAWGIAKETNA
                     TI"
BASE COUNT          666 a          576 c          573 g          544 t
ORIGIN      
        1 acacctgcca tccgtattgt cggcatcggt tgtcgccggt gcccgaagag acgttggtgg
       61 atgttgttag cgcgtcgcca gaaacacact ctgaaataga aaagcgcatc caaaaacaaa
      121 cctctggaaa cattgatatt tctcggctgg atacagtcgg ccaacggtct ttaagcaatg
      181 ttctcgaaga catttatgag cgatacccta atttgccgaa attagaatca gttgttgcca
      241 ctaatcggca tttaagcgat gaaaacgggg cacctatttt tgcaagcatg gataataagg
      301 agctaaaaat ttatgcaggg gctcaagata acgacaattt gcggcgcata ctcaaaagag
      361 agaaccgcga ccttaaagca attaacggta gaaccgctca ccattacgaa tccattgacg
      421 atgttattcg tcacgagttt ggtcactata tcgctgaaag caataatatg ataggtggca
      481 attatacaat tttcaccgga atggttgaaa atgcggggct tatgtcgcat ataatgacga
      541 acaagaatga attttttcca gaattgtttt tgctttttga aaaaggtgtt ttggcagaaa
      601 aactacccat agttgacgcc catatgcgcc aattactcac gaggatattc ggatgacaaa
      661 aggtgtttct caatgtcttt attgtacaca ttatcaatct atccgtcacg gcgaaaaaat
      721 accgcgctgt aaggcatttc ctgatggtat acctatgccg ttatttttta ccgaggtttc
      781 tcaccgcaaa ccctacccct tggacaacgg cattcaattt gagccgatag acgaactaaa
      841 agagagtgaa gagtaatgat tctcacctac tacctcgata gctccctcaa ccccgtgccc
      901 actggtaacg cattcggcag ggcgtcgaaa gtacacacct tgctgaactg gcacccgcgc
      961 tggcaatatc tctcacctaa cgagatgcgc cgaaaaatcg tcacagcatc cgcacagctc
     1021 gacaatctgt cgtacaaagg ctcgaaagca gacccagcac agccaatgca gtttccacgg
     1081 tcaatggggc aagacccagc gttcagtctc gacgagcaaa tacgccggct gctctctgcc
     1141 ttggtgcagc aagtcgagta caacctaaac cgcgcaggca tgggcatgcc gcaatacggc
     1201 cacggtaacg agcaattcac cgcacggcaa gacacgattt cacgcgaagc actgcaaaca
     1261 ctctatccct atctggcata atatgagaat acatcaaacg gtcatatctc gcaactcaag
     1321 aggagacata atacagtcta agaccaacat tgtctcttgc ctgcattttc gcgaagggcg
     1381 gtcgattgtc cgcaccggta cgggcgtgga cgacgtagca gaggcggatt tagtgagcgc
     1441 gacacagttg gaaatcggcg acttgatttt cgtgcaacgc ggctggcaac gcaacgatat
     1501 ttaccgcgtg atgacagtga gccccagctc tatgggccgc catcaataca aactcacaag
     1561 gaagttagac gatgccgcct gaaatgcaaa tcgttaaaga tatatgcgac ctgttcgccg
     1621 cgttgtcgcg acctatggcg tttcattttg gcgacactct cgaaaaaggc gaggttgacg
     1681 aagttctcaa aaatgcacct gtggcgcggt ggcttgtgcc agtagcgaac gacaaggttg
     1741 acgagttagc atgcagcaca gaggggttga ttcagacttt tagagcgagc ctcgttatcg
     1801 tcgcaaaaac tgcgtcgaat tgcacacaag agttgataga agttttacaa aaagtttata
     1861 ccacatcgga agacgtggcg ggcgtgcatg attcgccttc atccccacat gcgtggggaa
     1921 tagctaaaga aactaatgcg acaatatgac caatgcggtt catccccaca tgcgtgggga
     1981 ataggttgcg agccacgtcg tttctgcagg cgttttcggt tcatccccac atgcgtgggg
     2041 aatagggtac aggctactac aatgaggata cttgaaacgg ttcatcccca catgcgtggg
     2101 gaatagcatt gtctaactta atgcagacag gtctgtcacg gttcatcccc acatgcgtgg
     2161 ggaatagaca gacatagcgc aaaaattaga catggacaac ggttcatccc tacatgcgtg
     2221 gggaatagtt caaccggtac gctcgcccaa attttgtgaa cggttcatcc ccacatgcgt
     2281 ggggaatagg acgaagcccg ggtgaaccaa gaccagcttt gcggttcatc cccacatgcg
     2341 tggggaatag gtatccgcg
//
LOCUS       DAOMPD010000421         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_17990_length_2352_cov_2.144972, whole genome shotgun sequence.
ACCESSION   DAOMPD010000421 DAOMPD010000000
VERSION     DAOMPD010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17990_length_2352_cov_2.144972"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PLY93_12290"
     CDS             <1..884
                     /locus_tag="PLY93_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70306.1"
                     /translation="PQLEKVKEKLLETEIPKFKAEYENPYRVPSNDLIMLYAHDFASV
                     PPLTYKPFDPEAEAEEKLKAAELVLPGEETQGSGPKPQPGENLLNRFSSAFITARPLN
                     YIPNTGDSGDENDDAAGVPQVPQQITFRTFVQIVNTVSRFIKTQDKTGYAKYYQSLSP
                     LHQAAYTYYSLFQKEAQAQTVAWSDEIHKVMQKAQLDAGAANKLKDEVEAYRKVSQSI
                     TRALAQGQQKGMPAQLMQSLYGQLVTIFENNGNLAEKHTALKMSLLQVTVMPAKEAIT
                     QILTTQLNELENLYGLQ"
     gene            884..1930
                     /locus_tag="PLY93_12295"
     CDS             884..1930
                     /locus_tag="PLY93_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRP70307.1"
                     /translation="MGLEERLRAYNAVRPSINKPTGNEKKNSNVIETFYFDTHPVSGE
                     TLALPPKIAVDFRLDIHRENFLFFDLESTGLGSGEQVYPFLIGLADSQKSGAQLTTLF
                     ADTPADEEEILIQFLNAARGKTLVSFNGKSFDLPLILRRAEKYGIDARGLEKQHVDLY
                     HLIRRIFPEKPSRLIDAETRLLHFTRKDDLSGAAVAQAYFEMLQFKNDALKNKIIDHN
                     KWDVLSLLSLLLRVSVAFTHARRGEHLWAYKIHRDKSATHDEKKQLLIHAPHTLDARD
                     FYTLGQVYRREKDYHKAARAFLTSYRQGYANAAIDTVRALARLKKTTLAHSVARYALR
                     HEDERVQRHLMKYA"
     gene            1987..>2352
                     /locus_tag="PLY93_12300"
     CDS             1987..>2352
                     /locus_tag="PLY93_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HRP70308.1"
                     /translation="MIDKKVAAILDNTLDGKRMNHTDALYLWQHGDFKDLGNAADEVR
                     KKINPANEVSYTAFRVVNYTNYCNVECSFCSFMDEVGSGKGYTLSKEEIFAKLDEALR
                     LDAPQLFLQGGVNPDLPMTY"
BASE COUNT          664 a          620 c          538 g          530 t
ORIGIN      
        1 tgccgcaact cgagaaagtg aaagaaaagc ttctcgaaac tgaaatccct aagttcaaag
       61 ccgagtacga aaacccctac cgcgtgccgt cgaacgatct tatcatgctt tacgcacacg
      121 acttcgcgag tgtgccacct ttgacgtaca aaccgttcga tcccgaagcc gaggcagaag
      181 agaaactcaa agcggcagag ttagtgctcc ccggcgaaga gacgcaaggc tccggcccca
      241 aaccgcaacc gggggaaaac ttactcaacc gctttagctc tgcctttatc actgcgcggc
      301 ctttaaacta tatacccaac accggcgact cgggcgacga gaacgacgac gcagcgggtg
      361 ttccccaagt cccgcaacag ataacattcc gcacgtttgt gcaaatcgtc aacacggtga
      421 gccgctttat caaaacgcaa gataagacgg gttatgcaaa gtattaccaa agtctttcac
      481 ccctccatca agcggcgtac acctattact cgctttttca aaaagaagcg caggcgcaaa
      541 cggtggcgtg gtcggacgaa atccacaaag tcatgcaaaa ggcgcagctc gacgcgggtg
      601 cggcaaataa actcaaagat gaagtcgagg cataccgtaa agtttcacaa agtattacgc
      661 gcgcgcttgc gcagggccaa caaaaaggaa tgccagcgca attaatgcaa agcctttatg
      721 gtcaactcgt gactattttt gaaaacaacg gcaacttagc agaaaaacac acggcgctta
      781 aaatgtcact cttgcaagtg acagtcatgc ctgcgaagga agccattacc caaatcctta
      841 caacgcagct taacgaactt gaaaaccttt acggattaca ataatgggct tggaagagcg
      901 actacgcgca tacaacgcgg tgcggccttc gatcaataag cctaccggta acgaaaaaaa
      961 aaacagcaac gttattgaaa ctttttattt cgatacacac cctgtttcgg gtgagacgct
     1021 cgctttaccc cctaaaatcg ccgttgattt tcgcttagat attcaccgcg aaaattttct
     1081 tttctttgat ttagaaagca ctggcttagg ctcgggtgag caggtttatc cttttttaat
     1141 tgggcttgca gactctcaga aaagtggcgc gcagcttacc actctttttg ccgacacccc
     1201 cgccgatgaa gaagaaatcc tcatccagtt tttgaacgcc gctcggggaa aaacgttggt
     1261 ctcttttaat ggaaaatcgt tcgacctacc gcttatctta cgccgtgcgg aaaaatatgg
     1321 tattgacgcg cgtggcctcg aaaagcagca cgtcgacctc tatcatttaa tccgccgtat
     1381 ctttcccgaa aagccatcac ggcttatcga cgctgagacg cggcttctac attttacgcg
     1441 caaggacgac ttatccggcg cggcggtcgc tcaggcgtat tttgagatgc tccaattcaa
     1501 aaatgacgcg ctcaagaata aaattatcga ccacaacaag tgggatgtac tctcgctcct
     1561 ttctttactg ttgcgggtgt cggtggcgtt cacccacgcc cgcagaggcg agcacctgtg
     1621 ggcgtacaaa atacaccgcg acaagagcgc aacccacgac gaaaaaaagc agttgctcat
     1681 tcatgcaccg catacgttgg atgcacgcga cttttacaca ttggggcaag tgtaccgccg
     1741 tgaaaaagac taccacaaag cagcgcgtgc gtttttgacc tcttaccgtc aggggtatgc
     1801 caacgccgcg attgacaccg tgcgcgcttt agcgcggtta aaaaaaacaa ctctggcgca
     1861 ttccgtggcg cgctatgcac tcaggcacga agacgaacgg gtgcagcgcc acttaatgaa
     1921 atatgcttaa attgaccaca tagtacttta ccccctataa accatcttca gcgtttcgca
     1981 gactccatga ttgataaaaa agtagccgca atcctcgaca acacgttaga cggcaaacgt
     2041 atgaaccata ccgacgcgct ctatctttgg caacacggtg atttcaaaga tttagggaac
     2101 gccgccgatg aagtgcgcaa aaagataaat cccgccaacg aagtgtcgta cactgcgttt
     2161 cgtgttgtca actataccaa ttattgcaat gttgaatgtt cgttctgttc gttcatggac
     2221 gaggtcggct ctggaaaagg ctacacactc tctaaagagg aaatttttgc aaagctcgac
     2281 gaagcgttgc gtctggatgc gccacagctt tttttacaag gcggggttaa tcccgattta
     2341 ccaatgacgt at
//
LOCUS       DAOMPD010000422         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18037_length_2346_cov_2.422959, whole genome shotgun sequence.
ACCESSION   DAOMPD010000422 DAOMPD010000000
VERSION     DAOMPD010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18037_length_2346_cov_2.422959"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLY93_12305"
     CDS             complement(<1..211)
                     /locus_tag="PLY93_12305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP70309.1"
                     /translation="MNVAYNAGVDIAVPFGAQVQATAPGIVVSINREANFTYTVRIRH
                     NYGYETVYKGLEMVTVSQEEKVNKGE"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<248..1013)
                     /locus_tag="PLY93_12310"
     CDS             complement(<248..1013)
                     /locus_tag="PLY93_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70310.1"
                     /translation="MKKSAPERKKKKSSSQPKVHAVESKAISVTAEVLPPQPFLSAWA
                     MRGKETVKMAGPMLMHVSQSQQRIIAWVRRKKNERLTIMLMPHDRGSTRNYHISNLTL
                     IIITSILGIILLVSALLVIRHSSTIQEVDKLRVSHKDAEVQFAKVRQEIIDLSKSYED
                     IRQNLSSLQALSTEAGKSSGKTKDNFAEELAELQKKTSQIKDPEKIPLEIFLMNRMLH
                     DMEISRGSLGQVQDYLKKRDRMLKNTPTLWPVQGYII"
     gene            complement(1068..1724)
                     /locus_tag="PLY93_12315"
     CDS             complement(1068..1724)
                     /locus_tag="PLY93_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRP70311.1"
                     /translation="MKTTQIKKNYRIVLVDDHAILRSGIKMLIESKPGLEVVGELSNG
                     QELVTLMKEMPCDMVILDLNMPVMNGIETLGYLQENYPLVKSIVLTTHKEKPFLKRSL
                     SKGAHGYLLKEETHDDLLSAIHDVRQGKRAISKEMTNLIVDDFANDLVSPLSIDLLTQ
                     REKEILALTANGQTSKEVGERLEISARTVEAHRAHIREKLGLETHSELVRYAMEHHLI
                     "
     assembly_gap    1987..2086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          533 a          517 c          501 g          685 t
ORIGIN      
        1 gttcgccctt attgaccttt tcttcttgcg aaactgtcac catctcaagg cctttataca
       61 cggtttcata gccgtagttg tgccgtatcc gtaccgtata cgtaaaattt gcttctcgat
      121 tgatactcac cacgatgccg ggcgcagtgg cttgtacctg cgcaccgaaa ggtacagcga
      181 tgtcgacccc tgcattatac gctacgttca aacggtgagc cagccgtacg agaccgannn
      241 nnnnnnntaa taatataccc ttgcaccggc cacagcgtgg gggtgttctt cagcatgcgg
      301 tcgcgctttt ttaagtagtc ttgcacttgg cctaaagaac cgcgcgagat ttccatgtcg
      361 tgcagcatgc ggttcatgag aaaaatctcg agcggtattt tttcagggtc tttaatttgg
      421 ctcgttttct tttgcaactc agccaactct tcggcgaaat tgtcttttgt tttgccagag
      481 cttttgccag cctcggtact caacgcttgc aacgacgata gattttgtcg aatgtcttcg
      541 tacgatttgc ttaagtcgat aatttcttgc cgcactttag caaattgcac ctcggcatct
      601 ttatgcgaca cccggagttt atccacctct tggatagtgc tcgaatggcg gataaccaaa
      661 agcgcgctca cgagcaaaat aatgcctaaa attgaggtga taataataag agttaaattg
      721 ctgatatggt agttgcgcgt tgaccccctg tcgtgaggca tgagcataat ggtgaggcgc
      781 tcgttcttct tgcggcgcac ccacgcaatg atgcgttgct ggctttgcga cacgtgcata
      841 agcatggggc ccgccatctt tacggtttct ttcccgcgca tcgcccacgc gcttagaaag
      901 ggttggggag gtaatacttc agcggtgacg gagattgctt tactctctac cgcatgcact
      961 ttgggctgcg aactagactt tttctttttg cgctcagggg cagacttctt catggagaat
     1021 acgccagcaa gtgagctttg agtttgtgcg tcaaccgtaa acaggctcta aattaagtga
     1081 tgctccattg catatctgac gagttcactg tgcgtttcaa gccccaattt ctcgcggata
     1141 tgcgcacggt gcgcttcgac ggtacgcgcg ctaatttcaa ggcgctcgcc gacttcttta
     1201 ctcgtttgac cgtttgcggt taaagccaaa atttcctttt cgcgttgcgt tagtaaatcg
     1261 atggaaagcg gacttaccaa atcgtttgca aaatcgtcaa cgatgaggtt tgtcatttct
     1321 ttggaaatcg cccgtttacc ttgccgtaca tcgtggatgg ctgaaagtaa atcgtcatgc
     1381 gtttcttctt ttaggagata gccatgcgct ccttttgaaa gcgagcgttt caagaatggt
     1441 ttttctttat gcgtcgtgag tacgatcgat ttgactaaag ggtaattttc ttgtaaatac
     1501 cccagtgttt caatgccgtt catcaccggc atattgagat ccaggattac catatcgcac
     1561 ggcatttctt tcataagagt aaccagttct tgcccgttcg atagctcacc cacaacctca
     1621 aggcctggct tgctttcaat cagcatttta atccccgaac gtaatatcgc gtgatcatcg
     1681 acgagcacaa tgcggtaatt cttttttatc tgtgttgttt tcatttgcat cagaacctcg
     1741 tttttgcggt taaataatcg cattagattt tattcacata actgcaagtc tatataaaaa
     1801 aattaaaatt acaatccgtg tgattacgtt cttttttaag tagtattact tacgctaaac
     1861 agtcaaataa gccatgtata atgctggtac agcacacttt tgcaagttgg agtacgtaag
     1921 ttgtcaggca taactgctcc agtttttcgg attatgtcac attagggggt aactattcca
     1981 atttgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggat tatgtcacat
     2101 tagagggtaa ctattccaat ttgcggatta tgtcacatta gagggtaact attccaattt
     2161 gcggattatg tcacattagg gggtaactat ccgcgtttgc tttctagatt acgcctaaag
     2221 cgcatcccaa aactttcgtt gtacaacaaa gcgcgttcag cgtggaactt atcgcgtggc
     2281 gagtaatgag cggtcttgag atacgctcct aacccaatcg ttatttttta gtagcccgcc
     2341 agagaa
//
LOCUS       DAOMPD010000423         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18043_length_2345_cov_2.849782, whole genome shotgun sequence.
ACCESSION   DAOMPD010000423 DAOMPD010000000
VERSION     DAOMPD010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18043_length_2345_cov_2.849782"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..438
                     /locus_tag="PLY93_12320"
                     /pseudo
     CDS             115..438
                     /locus_tag="PLY93_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766237.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
     gene            470..>659
                     /locus_tag="PLY93_12325"
     CDS             470..>659
                     /locus_tag="PLY93_12325"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRP70312.1"
                     /translation="MKIAIAADHGGFELKEELKEALKKKGGIEVVDLGAFNAESVDYP
                     DFGAALARRVASGEFERGI"
     assembly_gap    660..709
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <710..913
                     /locus_tag="PLY93_12330"
     CDS             <710..913
                     /locus_tag="PLY93_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRP70313.1"
                     /translation="IKGIRAALCHNAYTAEMARKHNDANIIAMGGRIVDKATAIQMTE
                     IFLNTEFEGGRHARRVEKISALE"
     gene            876..>2345
                     /locus_tag="PLY93_12335"
     CDS             876..>2345
                     /locus_tag="PLY93_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70314.1"
                     /translation="MRGALKKLALWSNLKRALFLLSFAPFFATSLTNAQEGMATAPTQ
                     RKVFLARDNFLAALNENIERINLNDKVSALRLERKASTPKNLDDVLIDFEEDSLFTNP
                     AYTQSIRKAEFERVTHKGMNGRSAFFSSREHGLQIQLPTHLNLAGGGLTTEAGNFSFV
                     TEFNPTASNGEILRRENFVSGKQFLFSIRLLKGRVSVRLIHLIAEETSTDKKNYSSVE
                     LKSIDKIRLEQRNLLVFTYNEAQKKLTLTLNEREQEVYPLSKSGTYITFENLLSAPYS
                     LFVRYRGYADNILFSNSIFSENDVRDLGKLTPYGDTYTQRKGVIQSGIFDMRFSQSTV
                     TRIAVSAQSTSENWLEVLARCIDRRFDAELSQRDLPFLPINRIEGAKCRFIQFKSIFT
                     ADNAGKTSPLLKSITLEYRENPPPDAPLKPKVTALDSESVQVTISPNTELDVIKGGRY
                     IIYYGHTPQQIEGAVYFTDSPTTGLAHKVPLHVTVTNSIL"
BASE COUNT          645 a          567 c          474 g          609 t
ORIGIN      
        1 ccacctacct taaacacttt ataaaaagtg taacatatgt cttgagggtt tagaataagt
       61 tcttaattta ttacttgaaa cattatgtct cattgatgtt ttggattatc tcttatggaa
      121 cttaaagtcc tttcacttta tgcattcgct gcattctttg ctgcggcagg acttttgcat
      181 tttattaagc ctaaattttt tttgcgtatc atgcctccgt acataccatg gcataaaccg
      241 attgtatatc tcactggcat cgctgaaatt ggtttagcag tgttgcttct tatacctcct
      301 tatcgcgtat gggcagcgtg ggggatcatc gcacacctca ttgcggtttt tcccgccaat
      361 atttatcacc tcacctcggg gggagcgggc atgcgcatac cgcaatggct tttagtttta
      421 cgcctgccat tacaattatc ggttgggcat tctggcacac aaggtaaaga tgaaaattgc
      481 aattgcagca gaccacggtg gcttcgagct caaagaagaa ctcaaggaag cgttgaaaaa
      541 aaaagggggc attgaagtcg tcgatttggg cgccttcaac gccgaatcgg tcgattatcc
      601 tgatttcggc gcagcgcttg cgcgccgtgt cgcttccggc gagtttgagc ggggtatatn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttaaagggat
      721 tcgtgcggcg ctatgccaca acgcgtacac tgccgaaatg gcgcgcaaac acaacgatgc
      781 caatatcatt gcgatgggtg gtcgcatcgt cgacaaagcg acagcaatac aaatgacaga
      841 aattttctta aacacagaat ttgaaggcgg taggcatgcg cggcgcgttg aaaaaattag
      901 cgctttggag taacttaaag cgcgcccttt ttttgctgag ctttgcgcct ttttttgcga
      961 cttcacttac caacgcgcaa gaaggcatgg cgactgcccc tacgcaacgt aaagtattcc
     1021 ttgcccgtga taatttcctc gctgcattga atgaaaacat cgagagaatc aatttaaacg
     1081 acaaagtttc tgctttgcgt ttagaacgta aagcaagcac acccaaaaac ttagacgatg
     1141 tgcttattga ttttgaagaa gactctttat tcacaaaccc cgcatacaca caaagcatcc
     1201 gcaaagccga gtttgagcgt gttacgcata aaggaatgaa cggccgcagc gcttttttct
     1261 cttcgcgcga gcacggcctt caaattcaac tccccacgca tttgaacctt gctggcggcg
     1321 gattaaccac ggaggctggc aacttttcat ttgttaccga atttaacccc accgcctcta
     1381 acggtgaaat tctacggcgg gaaaattttg tctcggggaa acagtttctt ttctcaatcc
     1441 gtctcttaaa aggccgtgta agtgtacgcc ttatccacct tatcgccgaa gaaacatcca
     1501 cagataaaaa aaattacagt agcgttgaac taaaaagtat cgataaaatt cgcttagaac
     1561 aacgcaacct actcgtcttc acatataacg aagcacagaa aaaacttaca ctcactctaa
     1621 acgaacgcga acaagaagtt tatccgctta gtaagagcgg aacctatatt acttttgaaa
     1681 atttgctaag cgctccctac tcgctttttg tgcgctatcg gggttacgcc gacaacatac
     1741 ttttttctaa ttcaattttt tcagaaaacg acgtgcgcga tttagggaag ctcactcctt
     1801 acggagatac ctacacccaa cgcaagggcg ttatccaaag cggtattttt gacatgcgct
     1861 tttcacaaag tacagtgaca cgcattgccg tatctgcaca aagcacatct gaaaactggc
     1921 tcgaagtttt agcgcgctgt atagaccgcc gctttgacgc cgaattatct caacgcgatt
     1981 tacctttctt acccatcaac cgcatcgaag gcgccaagtg ccgcttcatt caattcaaga
     2041 gtattttcac agcagacaat gcgggaaaga cttcaccact cctaaaaagc atcactctcg
     2101 agtacagaga aaatcctccc cccgatgctc cgcttaaacc aaaagttacg gcattggata
     2161 gtgaaagcgt acaagtgacc atctccccca acaccgaact cgacgtcatt aaaggcggcc
     2221 gttatattat ttattacggc cacacgccac aacaaattga aggcgcagtg tatttcaccg
     2281 attcacccac aaccggattg gcgcacaaag taccattgca tgtcaccgtg acaaactcga
     2341 tactt
//
LOCUS       DAOMPD010000424         2343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18061_length_2343_cov_2.704545, whole genome shotgun sequence.
ACCESSION   DAOMPD010000424 DAOMPD010000000
VERSION     DAOMPD010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18061_length_2343_cov_2.704545"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLY93_12340"
     CDS             <1..290
                     /locus_tag="PLY93_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70315.1"
                     /translation="PSQKAANCSLPIVTYRLVGHKTHFSNLSGLGVYEIARGDGMNVR
                     VKVQAVAGQMNCAQDSRFKRFVDVCGSALPFRVNSCGEKIQKPRVDSSRKK"
     gene            complement(321..581)
                     /locus_tag="PLY93_12345"
                     /pseudo
     CDS             complement(321..581)
                     /locus_tag="PLY93_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025677.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(1381..1902)
                     /locus_tag="PLY93_12350"
     CDS             complement(1381..1902)
                     /locus_tag="PLY93_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70316.1"
                     /translation="MQKSRLIYYSLICVCIICARDILAEETSENKKLEVGAKNTLSLE
                     LLGIGGIYSVNYERSITKDALIRFGVAYLPPMRSTDNYHTLAAPLGFYYLIGQSEHKL
                     ELGFAGGFYGGYNKDPVTYGSEWKVRSAFFYSVGYRYQPVSSGIVFRITANLAFFDNI
                     AQPWGGISLGYAY"
BASE COUNT          608 a          588 c          509 g          638 t
ORIGIN      
        1 cgccgtcgca aaaagcggca aactgctctt tgccaatcgt aacgtatcgt ctggtagggc
       61 ataaaactca cttctctaat ctctccggtc ttggggttta cgagatcgcg cgtggcgatg
      121 gcatgaacgt gcgggttaaa gttcaagctg ttgccggcca gatgaactgt gctcaagatt
      181 cccggtttaa aagattcgtc gacgtctgcg gtagcgcgtt gccgtttcgt gtaaactctt
      241 gcggcgagaa gattcaaaaa ccgcgggttg attcttcgcg caaaaagtaa atccgccagc
      301 tgaccgggca tggtaaagac cacgtgaaag tgcattaacg ccggattcag cacctttgat
      361 aaatgaatcg accaaccgaa tacgcgttta cggtaacagg acaggcaaag gcggcttttg
      421 catgaaaaag gtaccgccaa gaccacttta catgattcac attcatgcca ttgaaagcct
      481 ctcccaaatt ggccacaggt taagagtttc ttcgcttcgc tgattttcca ttcgtcgatt
      541 ttgccgaatt tagcttcata gtgctctggg tacgcattca gaaaatcaga ccaatgcgtc
      601 tgccatatcg ccttgtagtg actataccgg gtcagtgcag gcctgtacgg tcgttgaccc
      661 gatttcgacg cgatgtaaat aaattgctcc ggagagcata taccaccctc tatatattaa
      721 ataccagaaa acggatgatt tttctcacaa tcatttcaaa aagacatttg tcactaaaac
      781 acgctatttt cgtcgttatg agggctaaat cttagtcgcc cggccctcct aaccgggctc
      841 acaggcgcaa ttacttcaga caacaaaatt tacccgctgc gctccctgcg gtgccgtagc
      901 tcctcgggtg ctcggccgct ctgccacaaa gatcgcggac aaagcacaag cgggacgctt
      961 gttttgtgct ttgccgcgag tggctccggg cacatttcgc cttgcggcga aacgtcgggt
     1021 aaatccaacg ttgtctgaca taattttctg cggctcctga aaactcccgc catccatggc
     1081 gggggcttat gcaaatggtt aatcaaaacg gccgcgggtg cagccgataa ctacgagttt
     1141 ccttatcgta gcaggtattt ttatcgtatt tgccagagca tcctttatgt tttttccctt
     1201 catttttcaa gatttgacct tcttgcgttg tttggctata gccacgaaag agcggcgcat
     1261 ttgaaccggg cactgctgaa ctcggtctct cggtgggagc ttgtcgggct tgagatattg
     1321 tgactgttga aataaatata gttatgatcc cagtcaggca tcgcgctgtg ctcccgttca
     1381 tcaatatgcg tatcctaaag aaataccacc ccaaggttgt gcaatattat cgaaaaaagc
     1441 taaatttgct gtaatccgaa agactattcc gctgctcacc ggttgatacc ggtatcctac
     1501 agaataaaaa aaagctgatc tcactttcca ttctgatcca taagttacag ggtctttatt
     1561 atatcctccg taaaacccac cagcaaatcc taactccaat ttatgctcgg actgacctat
     1621 caggtaataa aaacctaacg gcgcggctaa cgtatgataa ttatccgtcg atctcatagg
     1681 cggcaaatag gcaacaccaa atctgataag cgcatcttta gtgattgacc gttcataatt
     1741 tacggaataa atacccccta tccctaaaag ttctaaggaa agcgtatttt tcgccccgac
     1801 ctccagcttt ttgttttccg atgtttcctc cgctaatatg tctcttgcgc aaataataca
     1861 cacgcagatc agagaataat agataagtct tgatttttgc atcttgtcac ctttgcttag
     1921 tatgagtaag tcgccggatc gcagtagcta ccagtagagt agcaagcgct acaccagaaa
     1981 tagcaatcat cctctcccca aaaccagtga aaccaacatc cgggggtacg ataagtgtaa
     2041 gtgtggcata caggtggatc aggcggcgac aatgtgtcaa ctgtaatggt cggcggtaat
     2101 acggtcgttc ttacacctcc catatcccaa tatagccaac ccagattcac gcgcttccaa
     2161 gcttgaactc cctgcggtcc ggcgttattt tcaccgggtc caaaggtatt tttcgtctct
     2221 gtactattac agaatccgaa attcggatcg caggagtgcg aaattttata tgtcacaact
     2281 tccccctcgt gtcagcggat tctgtcgcct ttaattgact cgtaatttga tattttctcc
     2341 tca
//
LOCUS       DAOMPD010000425         2340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18098_length_2340_cov_2.103720, whole genome shotgun sequence.
ACCESSION   DAOMPD010000425 DAOMPD010000000
VERSION     DAOMPD010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18098_length_2340_cov_2.103720"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLY93_12355"
     CDS             complement(<1..435)
                     /locus_tag="PLY93_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70317.1"
                     /translation="MWNRKLAPLAVAFFLGCNNYGLLEQLQNPGGGNSAQNIMMYAFV
                     SSATMNGNLTGGGSLQSAFPPCSAFTGLQLADCACTQMAQNAGLAIPVNKYVAWLSTS
                     TNDMTCRISNTTSPLINCTLPGGGPTWVNMSGQTVASGYAALL"
     gene            complement(447..1406)
                     /locus_tag="PLY93_12360"
     CDS             complement(447..1406)
                     /locus_tag="PLY93_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70318.1"
                     /translation="MPGSLQEAITKSMHTKFEFVEVDAAKIEPIAAAVRAKNQGAITP
                     KDAADICRKADIDILIYGNFTFDDSTKEISIHTNISLGSTDKFRTLQPVKNRVDATIF
                     GAADRVAGDIVAEITKVALEQHKARGEEANKKGKTQLAKIEKAKTWADTNWMITVAGG
                     PILPLIFGGSATPKADAAIDAYIVRRLKEAWHLGVLGSFASIHSQNSSAEAVMNNVGA
                     GAVLGGYFFDLSPRWRATSAVGLGYYFGSFESYNFCRSCFNTTSTATSYKINNPFAIA
                     RLGIHFLIFSFLSIGLEADYRMYFDGGGPVQAGHGTLSISAVF"
     assembly_gap    1850..1859
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          582 a          595 c          601 g          552 t
ORIGIN      
        1 caaaagcgcg gcatagccgc ttgcgacggt ttggcccgac atattcaccc aagttgggcc
       61 tccgccggga agcgtgcaat ttatcaacgg gctcgtggta ttgctaatac ggcaagtcat
      121 atcgttcgtc gaagtgctga gccatgcgac atacttattt acgggaattg ccaagccggc
      181 gttttgcgcc atctgagtgc aggcgcaatc ggccagttgt agtcccgtga atgcgctgca
      241 cgggggaaag gcagattgca agctaccgcc gcccgttaaa tttccgttca tcgtcgccga
      301 agatacgaat gcatacatca ttatattttg cgcagagtta cctccgcccg gattttgaag
      361 ttgctctaaa agcccatagt tattgcaccc caaaaaaaac gcgactgcaa ggggggcaag
      421 ttttctattc cacatatatc ctcctattaa aaaactgccg aaatggatag agttccgtgg
      481 ccagcctgaa ccggcccgcc gccgtcgaag tacatgcggt agtcggcttc gaggccaatc
      541 gataagaaag aaaaaatgag aaagtggatt cccaaccgcg cgatagcaaa cggattgtta
      601 atcttgtaag aagtggccgt agaagtcgtg ttaaagcaag acctgcaaaa gttgtaagat
      661 tcgaaactac caaaatagta gccgagaccc acggcgctgg tcgcgcgcca acgcggcgaa
      721 aggtcgaaaa aataaccgcc cagaacggca cccgcaccga cattgttcat gacagcttcc
      781 gccgacgagt tctgactatg gatactggcg aacgaaccga gcactccgag atgccaagcc
      841 tccttaagac gacgcacgat ataagcgtcg atcgctgcgt cggctttcgg ggtggcgctg
      901 ccgccgaata tcaagggaag aatagggccg cctgcaacgg ttatcatcca attggtatct
      961 gcccatgttt tcgctttttc aattttagcc aactgggttt tacctttctt gttcgcctct
     1021 tcaccgcgag ctttgtgctg ttcgagcgcg acttttgtaa tctcggcgac gatatcgccc
     1081 gcgacgcggt cggcggcgcc gaaaatggtc gcatcgacgc ggtttttgac gggttgcagt
     1141 gtacgaaatt tgtcggtcga gccgagcgaa atatttgtat ggatggagat ttcttttgta
     1201 gaatcgtcga aagtaaaatt accgtatatc aaaatatcga tatcggcctt acggcagatg
     1261 tcggctgcgt ctttgggcgt aatcgcaccc tgatttttag cgcgcacggc ggcggcaatc
     1321 ggttcgatct tcgccgcatc gacttcgaca aactcaaatt tcgtgtgcat cgactttgtg
     1381 atagcttctt gcaaagaacc gggcatatac ttgaaattcg ccgtgccggt cttgtcgtcg
     1441 tattgaatga tggcaacgcg cgccgtatcg gtagccggtg cggcaatgag agaagacgac
     1501 agaaagagaa aatgccgatg aggcttacca acttcataaa aatataagac gagttcgacg
     1561 tcgtaatgtc agcaaaaatt atttgtgtcc ggagtaagga gcctgtgcgc cttgccgcga
     1621 tgcacgaaag cgcacatctc tgtgattttg aatttcaatt tcaactgaaa agttgctatg
     1681 caacgtgaat acggcaggct atgcgtgccc atggttacgc gcgttatgcg tagcatcact
     1741 tacgctattt gctttctact cttagcgatt tgcaccgcct gccagtcaca aggctggggt
     1801 aagttttgga aaacgaccaa agttacacca acatctgcac ctgcgtctgn nnnnnnnnnc
     1861 cctgacgatg atttccgtgc ccgccaatac agcgggtttt cttatgggct cagcggatat
     1921 cggtggaaca gtcatgccag agcataccgc cgcgtccatc accgtctccg cgatggtcaa
     1981 gtacgaagtc caatacggcg actggcttat gtgaaaacct gggctacgag caacggctat
     2041 tcgtttgcca atccaggcgc tcagggaaac gatggtgcgc gcactaatca acatcccgtg
     2101 acgaaagtga actggcgcga cagcattatc tggcgcaatg cggcctcgca ggaagctggc
     2161 ctaacgcccg tgtattacac ggatgccggg tttaccatgc cattaaaaac atcgacgaac
     2221 accgtctcaa tgaatggtac tgccggtagt gaagacagac ccttcgtcaa ttggtgcgaa
     2281 tgcctatcgc ctgcccactg aaatgggctt accttgtgtt tttttaaccc gctcaacaaa
//
LOCUS       DAOMPD010000426         2339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18106_length_2339_cov_2.278021, whole genome shotgun sequence.
ACCESSION   DAOMPD010000426 DAOMPD010000000
VERSION     DAOMPD010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18106_length_2339_cov_2.278021"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>753
                     /locus_tag="PLY93_12365"
     CDS             <1..>753
                     /locus_tag="PLY93_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRP70319.1"
                     /translation="IAESGIVADDVCAFLGTLRTREKVLERRSQARKLSALKTFYNFL
                     EKRSLIAKNPTRTLKAARYKRLLPRPLRPIELERLLDDPVDDEEHFTGMRDKALWETI
                     YSSGMRISEALSLSVTPFSFSVIPDEITVTGKGSKDRVVYFGKCARQAITEYLPLRSA
                     VLSRLKKQSDALFLNFKATPLTRRGAHYLIQKRARALGLATRVTPHTLRHSFATDLLN
                     EGANIRHVQEMLGHASVSTTQNYTHVAKERLF"
     assembly_gap    754..777
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<778..1059)
                     /locus_tag="PLY93_12370"
     CDS             complement(<778..1059)
                     /locus_tag="PLY93_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70320.1"
                     /translation="MRAEDQVWGGIYAIIYRLGEIAIKRKRTKCTQIKLPPERKQQRV
                     LEWKNRPPGIKQSGQAGWPVFSKISGGTGSLIEFLDMWIYLFLCGCDLRW"
     gene            1058..1417
                     /locus_tag="PLY93_12375"
     CDS             1058..1417
                     /locus_tag="PLY93_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRP70321.1"
                     /translation="MEKHGIREADLEESFIRSGGKGGQNVNKVSTAVRLSHPTSGIEI
                     KCMKHRTQGMNRYAARVLLCEKLEQKERDTNAIENPAHKKKRLSKLNRARKAKRKAKA
                     KAEKKPKQPDWRKSGEW"
     gene            complement(1443..1658)
                     /locus_tag="PLY93_12380"
     CDS             complement(1443..1658)
                     /locus_tag="PLY93_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70322.1"
                     /translation="MAIAFSAYLYTDRAHRELTEADIEKISSAYHAWRDDRFLRQAQG
                     TKRPLVATRFGSPSSESVETSDKKELV"
     gene            complement(1814..>2339)
                     /locus_tag="PLY93_12385"
     CDS             complement(1814..>2339)
                     /locus_tag="PLY93_12385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRP70323.1"
                     /translation="MMITGWARPAAIGDEDKKFEVLYRLLGDGQTSRLVDRLVVREKL
                     AGSVKIWSTTPGDKLSSEFAIFIDVFDAASYNKIENIIQEELDRLAKEGPTENELKKI
                     KNRYAAELIQALQKNAGMADALSYYDLLLGDYHRMFTALEEIESVNAVQIQELIKKYF
                     IPAQNTTVYINPIQ"
BASE COUNT          601 a          607 c          563 g          544 t
ORIGIN      
        1 aaatcgcaga aagcggcatc gtggccgatg acgtgtgcgc gttcttggga actctgcgca
       61 cgcgcgaaaa agtactcgaa cgccgttcgc aagcgcgcaa actctcagcg ctcaaaacgt
      121 tctacaattt tcttgaaaag cgttcactca ttgcaaaaaa tcccacgcgt acgctcaaag
      181 ccgcacgcta caaacggctt ttgccgcgcc cactgcgccc cattgaactc gaacggcttc
      241 tcgacgatcc ggtggacgac gaagaacatt tcaccggcat gcgcgacaag gcgctttggg
      301 aaacgattta ttcgtcgggc atgcgtattt cagaagcgct gtcgctttca gtgacgccgt
      361 tttcttttag cgtcatcccc gatgagatca ccgtgacggg taaggggagt aaagaccgcg
      421 tggtttattt cggcaaatgt gcgcggcaag ccatcaccga atatctgccc ctgcgcagcg
      481 cggtgttgag tcggctcaag aaacaaagcg atgctctctt tttaaatttc aaagccactc
      541 cgctcacccg acgcggtgcg cactatctca tccaaaagcg cgcacgcgca ttgggcctcg
      601 caacgcgcgt tacaccgcac acccttcggc attccttcgc caccgattta ttaaacgaag
      661 gcgcaaacat ccgccatgtt caagagatgc tggggcatgc aagtgtttcc accacacaga
      721 attatacgca cgttgcaaaa gaacggctct ttgnnnnnnn nnnnnnnnnn nnnnnnncca
      781 ccgcaagtcg cacccgcata ggaagaggta aatccacata tctaaaaatt cgatgagcga
      841 gcctgtgccg ccagaaattt ttgaaaaaac tggccaacct gcttgaccgg actgctttat
      901 accggggggc ctatttttcc attcaagaac ccgctgttgt ttgcgttctg ggggtagctt
      961 tatctgggta cattttgtcc gttttctctt tattgcaatc tccccaagcc tgtaaataat
     1021 cgcgtagatg ccaccccaga cctggtcttc ggcgcgcatg gagaaacacg gcatccgcga
     1081 agccgacctc gaagaatcgt tcatccgcag cggcggcaag ggcgggcaga acgtgaacaa
     1141 agtttcgacg gcggtgcggc tatcgcaccc gaccagcggt atcgaaatca agtgcatgaa
     1201 acaccgcacg cagggcatga accgctacgc cgcgcgggtc ctgctatgcg aaaaactgga
     1261 acagaaagaa cgcgacacca acgcgattga aaaccccgca cacaagaaga agcggctgtc
     1321 aaaactcaac cgcgcgcgca aagcaaagcg caaggccaag gcgaaagcgg aaaaaaaacc
     1381 aaaacaacca gattggagaa aatcggggga atggtgatga gcaatatttt cgatcttgag
     1441 ggttaaacca attccttttt gtcgctcgtt tcaacagact ccgaagacgg agacccaaag
     1501 cgggtcgcaa cgagcggtct ctttgttccc tgagcttgtc gaaggaaacg atcatcacgc
     1561 caagcatgat aggcgctgga gattttttca atatcggcct ccgtaagctc gcggtgtgcg
     1621 cgatcggtat agaggtaggc agaaaatgca attgccaatt ggtaagtcaa acaaagtgta
     1681 aatccaacat tatgcaaccc atatgcaacc cacagagaac ccatgcgcca actcgcgcag
     1741 cgtgtataaa agcaattttt ttgaaaattt ttgtgcgcag ttaacgcaca ggcttctatt
     1801 ggctcattcc gtattattga atcggattta tatacaccgt cgtattttgc gccggtataa
     1861 aatacttctt tattagctct tgaatttgta ccgcgttgac actctcgatt tcttcgagtg
     1921 cagtgaacat gcggtggtaa tctcccaaaa gtaaatcgta atacgaaagt gcgtcggcca
     1981 tgcctgcatt tttttgtaac gcttggatga gctcggcggc gtagcgattt tttatttttt
     2041 tgagttcatt ttctgtgggg ccttctttcg cgagacgatc gagttcttct tggatgatat
     2101 tctcaatttt attatacgac gctgcgtcga acacgtcgat gaagatggca aactcagagg
     2161 atagtttatc gcccggagta gtcgaccaaa ttttcacgct gcctgcaagt ttttctcgta
     2221 cgaccaggcg atcaaccaag cggctggttt ggccgtcgcc taaaagtcga tacaaaactt
     2281 caaatttttt atcctcgtcg ccgatggcgg cagggcgtgc ccaaccagtg atcatcatc
//
LOCUS       DAOMPD010000427         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18128_length_2337_cov_2.388694, whole genome shotgun sequence.
ACCESSION   DAOMPD010000427 DAOMPD010000000
VERSION     DAOMPD010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18128_length_2337_cov_2.388694"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLY93_12390"
     CDS             complement(<1..479)
                     /locus_tag="PLY93_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRP70324.1"
                     /translation="MKKIPYTTNSVEDLLTLARDLRDKDGGCPWDIEQTHQSLAKYLL
                     EESSELIDVIEAGITQANIHDFKEELGDVLFQVVIHAQLAQENNFFNFYDVARTVTDK
                     LTSRHPHVYGSTQAKNTQQVLNTWEKIKKEEREKKQSLEENLPSAGRSDNLWPNHSF"
     gene            566..1528
                     /locus_tag="PLY93_12395"
     CDS             566..1528
                     /locus_tag="PLY93_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HRP70325.1"
                     /translation="MAVASANNQKKISYRAKEVSKCPVCGTTHFKEELLSGSGRLIAG
                     KLTQELQRLYEENKKWGKINPLVYHVQVCPKCLFAAFPKDFLSVNPEEVLAIKNTIAH
                     RQKVVETFFGKYDMGENRHLVHGAISYMLAVDCYHLRGNAVAPTPKKAVAALRSAWLL
                     DDLYKEADYRPYDKLRDFYYVEAARYYKRVLDTMTAGIEPMEEAAYILGPSLDFNWAF
                     DGVIYLNSYLTRKFIEQMATNPKDKYTIMDSSRRYLSKLYGSGKSSKSRPSVIIDMAK
                     ELYDEMGKMMEKYRCEYDPAFAEQKAKEAAEAAALVAEAQGRAK"
     gene            complement(1789..>2066)
                     /locus_tag="PLY93_12400"
     CDS             complement(1789..>2066)
                     /locus_tag="PLY93_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRP70326.1"
                     /translation="CVDEKSPIQALQRSQPNLPMRPGSPERQTHDYHRHGTTTLFAAL
                     DVVSGKVLGQCKERHRASEFIDFLKHIDANTPRKMELHSISDNFSAR"
     assembly_gap    2067..2076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          608 a          505 c          561 g          653 t
ORIGIN      
        1 caaaaactat ggtttggcca aagattgtcc gacctccccg ccgagggtaa gttttcttct
       61 aagctttgct ttttttcgcg ttcctctttt ttaatttttt cccaagtatt tagtacttgc
      121 tgagtattct ttgcctgagt gctaccatag acatggggat gccgcgaagt gagtttatcc
      181 gtcacagtac gagcgacatc gtaaaaatta aaaaaattat tttcttgagc gagttgtgcg
      241 tgaataacca cttgaaagag tacgtctccc agctcttctt taaaatcatg gatgtttgct
      301 tgggtgatgc cggcttcgat aacatcgata agttcactcg attcttccaa aagatacttg
      361 gctaaacttt gatgggtttg ttcaatatcc catggacaac caccgtcttt atcgcgtaag
      421 tcgcgtgcaa gagtgagtag gtcttcgaca gaatttgttg tgtatggtat ttttttcatg
      481 caccagtttt tgaagaagat tgcatgcgac agtaaaaatt caatcggtct tgacacgttg
      541 tttttatgaa ccatccatgc aaggtatggc agtagcatcg gcgaacaatc aaaagaaaat
      601 ttcatatcga gcaaaagaag tgtccaaatg ccctgtgtgt ggcacgactc actttaaaga
      661 agaattactt tccggatcgg gacggcttat tgcggggaaa ctcacccaag agttgcaacg
      721 cctctatgaa gaaaataaaa agtggggaaa aattaatcct ctcgtgtatc atgtacaggt
      781 ttgtcccaaa tgtctttttg cggcgtttcc caaagatttt ctttcggtaa atccagaaga
      841 agtccttgcg ataaaaaaca cgattgccca taggcaaaaa gttgttgaaa ctttttttgg
      901 taagtatgac atgggggaga accggcatct tgtgcacggt gcgattagct atatgctcgc
      961 cgttgattgc tatcatttac gtggcaacgc ggtcgctcca actcccaaaa aggcagttgc
     1021 tgcgctgcgt tcggcatggc ttctagacga tctctataaa gaagcagatt accgccccta
     1081 cgacaagctt cgcgattttt attatgtcga agcggcgcgg tactacaaac gagtgttaga
     1141 tacaatgacg gcgggcattg aaccgatgga agaagcggcg tatattttag ggccatcttt
     1201 agattttaac tgggcgtttg atggggttat ttatttgaac tcctatttga cgcgtaagtt
     1261 catcgaacaa atggcgacga accccaaaga taaatataca attatggaca gctcgcgtcg
     1321 ttatctctca aaactttatg gcagtggtaa atcctccaag agtcgcccat cggtgattat
     1381 cgacatggcg aaagaattat acgacgagat gggtaaaatg atggagaaat accgttgtga
     1441 atacgaccct gcgtttgcgg aacagaaggc gaaagaagca gcagaagccg cggcattggt
     1501 ggccgaggcg caagggcgtg caaagtaacg cgatcggtag gatgccttct ttgttacgta
     1561 aagcacaagg aggtgcttgg ttcgtgtgca acaggatgtt gctctttgta cgaaccgcca
     1621 agcgcggaag ctgaccaaga gtcgatagtc tcctgtaatt aagaaaatgc cgcaattttt
     1681 gtgctgatat ctgcggcttt tttcgtccag cgaaaaggtt ttgggtcttt attccatccg
     1741 cgtatgtacg cgtatatggc cattgtaagg tcgcgcaccg aagtccagtc accgcgccga
     1801 aaagttgtct gaaatgctat gcagttccat tttgcgtggc gtattcgcat cgatatgttt
     1861 caggaaatcg atgaactcgc tagcacggtg gcgctcctta cattgaccta agactttacc
     1921 ggaaacgacg tctaatgcag cgaacagagt cgtcgtccca tgccggtgat agtcatgcgt
     1981 ttgccgttcg ggactgcccg gacgcatcgg taaattcggt tgcgagcgct gcaatgcctg
     2041 tattggggat ttctcatcaa cgcagannnn nnnnnnctgg gtgcagcaga aaaccattga
     2101 ttttgcaggt ggattcaggt ataaaccaat aacatcagcc atcgtgcgtg tagtccagtg
     2161 cgttacattc tttggcttct cctgaaccgt cttgcagcaa ggcttcaatt caatataccc
     2221 ttacccaacg actaatctgc gttctatcga cccgcaatac gtgcgcaacc tgggctactt
     2281 ttaggccatc acttcgggca agcactgctt gaacgcgctt caattgtttt ggcttga
//
LOCUS       DAOMPD010000428         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18147_length_2335_cov_2.514474, whole genome shotgun sequence.
ACCESSION   DAOMPD010000428 DAOMPD010000000
VERSION     DAOMPD010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18147_length_2335_cov_2.514474"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>795
                     /locus_tag="PLY93_12405"
     CDS             12..>795
                     /locus_tag="PLY93_12405"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRP70327.1"
                     /translation="MNKFLDILNAAYKFVETGADVSWVRRFTALGKAYLSGVESDITY
                     VPNKKDLSEALQNKIMSAQRAAGKIRSKSTVISCGFGAMARPSVFFKALKFIAEKNKS
                     PLRLTWVTVSAAGARGRAAGSLEDLAIPGLIERYITGHVETVKAFVPLVSGGQMAWDM
                     LPQGVITQVLVAQAQNKNFVETETGLGTYVEKQIDDGATNLVTRSGDKLRYTLPRIGV
                     AIVVAASVDNEGNISAEGAASLTEMADAARAAEGPGIFREGAP"
     assembly_gap    796..805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <806..1783
                     /locus_tag="PLY93_12410"
     CDS             <806..1783
                     /locus_tag="PLY93_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70328.1"
                     /translation="RAAKANGGIVIAVVGKVVDSFTAERLLSSHEVDYIIVDPYAEQI
                     GGIPLNDAWSLFLPRTQSVSAADFKEAYAALRLINKVAGITSERDASAQMVARLGARV
                     LQENIKEGALVNLGVGLPEEVAGIYCDEGRSEEVLFTVEGGAMGGLPAPGVFFGASLY
                     PQKLTSSTTTFARYREGLDATVLGFLQIDAEGNVNVSRRGADGSAKTAIGPGGFIDIC
                     EGAKLIIFMGSSRVRGKVEITPNGVKILPGGKPKLVPRLDEVTFSAKRALQKGKQVFY
                     ATDFGLLRLTENGLELIEVFRGIDIERDIERTQLIPIDVSRVKKRRIQI"
     gene            1844..>2335
                     /locus_tag="PLY93_12415"
     CDS             1844..>2335
                     /locus_tag="PLY93_12415"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRP70329.1"
                     /translation="MGSSIGRLFRVTTFGESHGEALGCVIENCPAGMPLTVHDIQGDL
                     DRRRPGQSRVTTPRSEPDTARIVSGTFEDKTIGSPIAITVFNENAREKDYSDIKEIFR
                     PSHADFTYQERYHHRAWRGGGRASARATIGVVAAGAVAKKLLREKAGVEIVGYVSQIK
                     DIFY"
BASE COUNT          576 a          566 c          638 g          545 t
ORIGIN      
        1 cgtgtagagc catgaataag tttctcgata tcttaaacgc agcgtataaa ttcgtcgaaa
       61 ccggcgcgga tgtctcttgg gtacgccgtt ttaccgcttt aggtaaagcg tatttaagcg
      121 gcgtcgaatc ggacattact tatgtaccga acaaaaaaga tttaagcgaa gcattacaga
      181 ataaaatcat gagtgcgcag cgcgcagcgg gtaagatacg ttcaaaatcg acggtcatca
      241 gttgtgggtt tggcgcgatg gcacgcccga gtgttttttt taaagcgctt aaatttattg
      301 cagaaaaaaa taaatccccc ctccgcctca cttgggtgac ggtgagcgct gccggtgcgc
      361 gtggccgcgc tgcgggctca ctcgaagact tggcgatccc gggccttatt gagcgttaca
      421 tcacaggtca cgttgaaacg gtgaaagcat ttgtgcctct cgtgagcggt ggccagatgg
      481 cgtgggatat gctgccgcaa ggcgtcatca cgcaagtgtt ggtggcgcaa gcacagaaca
      541 aaaattttgt ggaaaccgaa actggcctcg gcacgtatgt tgaaaaacaa atcgacgatg
      601 gtgcgacaaa tcttgtcaca cgttctggcg ataagttacg ctacacatta ccgcgtatcg
      661 gtgtagccat cgtcgtcgct gccagtgtgg ataacgaagg caatatctcg gcagagggcg
      721 cagcgtcgct caccgaaatg gccgatgccg cgcgtgcagc agaaggtcca ggaatttttc
      781 gggaaggagc cccacnnnnn nnnnncgtgc agcaaaagcc aacggcggca ttgtgattgc
      841 cgtggtgggt aaagtggtcg atagttttac cgctgaacgc cttctttcgt cacacgaagt
      901 cgattacatc attgttgacc cctacgccga acaaattgga ggcattccct taaacgacgc
      961 gtggtctttg ttcttgcctc gcacgcagag tgtgagcgca gcagacttca aagaagcgta
     1021 cgctgcgctc cgcctcatca acaaagtggc ggggattacc tctgagcgcg acgcctctgc
     1081 gcagatggtt gcgcgtttgg gtgcgcgggt actccaagaa aatatcaaag aaggcgcgct
     1141 ggtcaattta ggcgtgggac ttcctgaaga agtggctgga atttattgcg acgaaggacg
     1201 cagcgaagaa gtattgttca ctgttgaagg tggggcaatg ggtggactac ccgcaccggg
     1261 tgtttttttc ggcgcctcgc tttatccgca aaaactcact tcttccacta ccacatttgc
     1321 gcgttaccga gaagggctcg atgcgacagt attgggtttt ttacaaatcg acgccgaagg
     1381 gaatgtaaac gtatcgcggc gtggcgcaga cggttcggca aaaaccgcca tcggccctgg
     1441 aggtttcatt gatatttgcg aaggcgcaaa gctcattatt tttatgggct cgtcacgcgt
     1501 tcgcggcaaa gtagaaataa cacccaatgg cgtaaaaatt ttgccaggcg gaaaacccaa
     1561 gctcgtgccg cgtttagacg aagtgacgtt tagtgcaaag cgtgcgctcc aaaaaggtaa
     1621 gcaagttttt tacgcaaccg actttggcct cctgcgttta accgaaaacg gtcttgagct
     1681 catagaagtt tttcggggga ttgatatcga acgcgatatt gagcgcactc agcttatacc
     1741 catcgacgtc tcacgcgtga aaaaaagacg gattcaaatc taactcccgt tgtgttccct
     1801 tacgtttacg gcctcactcc attctcgcaa atgtccgtaa tttatgggta gttcaatcgg
     1861 tcgtcttttc cgcgtcacga ccttcggcga atcgcacggt gaagcgttag ggtgcgtcat
     1921 tgaaaattgt cctgcgggta tgccgctcac agtgcacgac atccaagggg atttagaccg
     1981 acgtcgccca gggcaaagtc gagtgacgac gccccgcagc gaacccgata ccgcgcgtat
     2041 tgtatcaggc acgtttgaag ataaaaccat tggcagcccc attgcgatta ctgtctttaa
     2101 cgaaaacgcg cgagagaaag attatagcga catcaaagaa atttttcgcc ccagccacgc
     2161 cgacttcact taccaagaac gctaccacca ccgtgcatgg cgtggcggtg ggcgtgcttc
     2221 ggcgcgagcg acgattggtg tcgtggcggc aggtgcggta gcgaagaaac tcttgcgcga
     2281 gaaagcgggc gttgaaatcg tcggttatgt atcacaaatt aaagatattt tttat
//
LOCUS       DAOMPD010000429         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18162_length_2334_cov_2.392277, whole genome shotgun sequence.
ACCESSION   DAOMPD010000429 DAOMPD010000000
VERSION     DAOMPD010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18162_length_2334_cov_2.392277"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /gene="flgC"
                     /locus_tag="PLY93_12420"
     CDS             <1..453
                     /gene="flgC"
                     /locus_tag="PLY93_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRP70330.1"
                     /translation="ESINVASTGLTAQRLKMDVISNNIANASTTRTNEGDGAYHRRRV
                     VMQPVNVRTRWKSPVYPFGLRAGDGEGVRVLKIERDETTPMRMVYDPSHPDAIKVGPK
                     KGYVEYPNVDIVSEMVDMISASRSYEANSQVIQGAKSMFQAAMQIGRA"
     gene            463..807
                     /gene="fliE"
                     /locus_tag="PLY93_12425"
     CDS             463..807
                     /gene="fliE"
                     /locus_tag="PLY93_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRP70331.1"
                     /translation="MLPITENFFHQNNVHGDYVALKTSDERHNNGRAIKVMGEDAALN
                     FSDALVKALEKVNAQQVSAETLGQKVVSDPTGVEAHTLMIQAEKARMSLTLVKNIADL
                     AVRTYRELSNLR"
     gene            958..>2334
                     /gene="fliF"
                     /locus_tag="PLY93_12430"
     CDS             958..>2334
                     /gene="fliF"
                     /locus_tag="PLY93_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRP70332.1"
                     /translation="MPPFVKRILSRLKELSSKLDSTKKIILGSAVAVVLVALVIIGNV
                     SSAKSSVVLFKSMETKDFAAVTAKLSEMGFHHTTSGTDTIFISPDKRNAAMVALAQEN
                     LIPQGVHGWELFDIDKWSETQFEKDIKKQRALSGAIASTLRQVKGVEKAQVNLAFPAE
                     ELFQEKSEPVTAAVLLEYKPGVEKLSTKEIKGIETLVARSVPGLKKENVTIAGPDGDV
                     INDFDNEIDKQKAEIKDVEAKLRIQEAQRTKLLADIRKSLTKFYGEGVYGDRYDVVRL
                     DAKLRWDKEELERNEVEPVVMTPDDPKTPYSEREVKDSLEVSSKTTTEEFDGNGFTPE
                     GPAGTEPNIPPGYKDRDYQKAKYVKKETIKNNKFNETHRKIKKQPWELERVNLAVLLD
                     GKWERLGVKADQSGYERKYLGVTDEELLKVTDVLKKSIGFDIGRGDQISVKHLQIDRS
                     KQFEAEDEE"
BASE COUNT          725 a          512 c          587 g          510 t
ORIGIN      
        1 gaatcgatta acgtcgcatc caccggactt accgcgcagc gtctcaagat ggacgtcatc
       61 tccaacaaca ttgcaaacgc atcgacgacg cgcaccaacg aaggcgatgg tgcgtaccac
      121 cgccgccgcg ttgtcatgca gccagtcaac gtacgcaccc gctggaagtc tcctgtatat
      181 ccatttggct tgcgggcagg cgacggggag ggtgtgcgcg tcttgaaaat tgagcgcgat
      241 gaaaccactc cgatgcgcat ggtgtacgat ccatcgcatc ccgatgctat taaagtaggt
      301 cccaaaaagg gatacgttga atatcccaac gtggacattg tttcagaaat ggttgatatg
      361 atttcagcta gccgcagcta tgaagcaaat tcacaagtca tccaaggcgc aaagtcgatg
      421 ttccaagcag cgatgcaaat aggtagagca taggagtaaa caatgttacc aatcactgag
      481 aatttttttc atcaaaacaa tgtccacggc gattacgttg cgcttaaaac ttccgatgaa
      541 cgccataaca acggacgggc gatcaaagtc atgggcgaag acgctgctct caatttttcg
      601 gatgctttgg taaaggcgtt agaaaaagtc aacgcgcaac aagtgagcgc agaaacactg
      661 gggcaaaaag ttgtctccga ccctacaggc gtcgaagcgc acacactcat gattcaggcg
      721 gagaaggcgc gcatgtcgct cacgctggtg aaaaatatcg ccgacttggc ggtgcgtaca
      781 taccgcgaac tttctaactt gagatagttg gatggctaac gaagcaaata aaattttggg
      841 gtggggctgt accactctca tgagcgcagc aattaaaaga tggttgcgct catgcgtgct
      901 tgtaaaattt ttgaaaaaaa gatgtgacat aataaaagga taaaaaagga gtacactatg
      961 ccgccgtttg taaagagaat tcttagtcgc cttaaagaac tgtcgagtaa gctcgacagc
     1021 actaaaaaaa taattttagg ctcggcggtg gcggtcgttc ttgtcgcgct cgtgattatc
     1081 ggcaatgttt cctctgcaaa atcgagtgtc gtgctcttta agagtatgga aacgaaagac
     1141 tttgcagcgg tgaccgccaa gctctccgaa atgggatttc atcacaccac atctggcacc
     1201 gacaccattt tcatttcacc cgacaagcgt aacgcggcga tggttgcact tgcccaagaa
     1261 aacttaatcc cgcaaggcgt ccacggctgg gaactttttg acatcgacaa atggtcagag
     1321 actcaatttg aaaaagatat taaaaaacaa cgcgcgcttt caggcgcgat tgcgagcact
     1381 ttgcgccaag taaaaggcgt tgagaaagcg caagtcaatt tggctttccc cgccgaagaa
     1441 ttgttccaag aaaaaagcga gccggtgact gcagcggtgc ttttagaata taaaccgggt
     1501 gtcgaaaagc tgtcaacaaa agagatcaaa ggtattgaaa cgttggtcgc acgttcggta
     1561 ccgggactca aaaaagaaaa tgtgacaatc gcagggccgg atggcgatgt gattaacgac
     1621 tttgataatg aaatcgataa acaaaaagca gaaattaaag acgttgaagc gaaactgcgc
     1681 atccaagaag cgcaacgtac caaactcttg gctgacattc gtaagtcgct cacaaaattt
     1741 tatggcgaag gcgtgtatgg cgatcgttac gatgttgttc gtctcgatgc caagttgcgt
     1801 tgggataaag aagagctgga aagaaatgaa gtcgaaccgg tagtcatgac gcccgacgat
     1861 cccaagacgc cgtactcaga acgcgaagta aaagactctc tcgaagtttc gtcgaagaca
     1921 accaccgaag agtttgatgg aaacggtttt actcccgaag ggccagcggg tactgagccc
     1981 aacattcctc ctgggtataa agaccgtgat tatcaaaagg cgaagtacgt aaaaaaagaa
     2041 acaatcaaaa ataataaatt taacgaaacg caccgcaaaa ttaaaaagca accgtgggaa
     2101 ctcgaacgtg tgaacctcgc ggtactcctc gacgggaaat gggagcgttt gggcgttaaa
     2161 gcggatcaat ccgggtatga aagaaagtat ttgggcgtta ctgacgaaga attattaaaa
     2221 gtaaccgacg tgctcaagaa gtcgattggt tttgatattg gccgaggcga ccaaatttct
     2281 gtaaagcatt tacaaattga tcgtagtaaa caattcgagg cggaagacga agag
//
LOCUS       DAOMPD010000430         2332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18180_length_2332_cov_2.825648, whole genome shotgun sequence.
ACCESSION   DAOMPD010000430 DAOMPD010000000
VERSION     DAOMPD010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18180_length_2332_cov_2.825648"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..771
                     /locus_tag="PLY93_12435"
     CDS             106..771
                     /locus_tag="PLY93_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70333.1"
                     /translation="MKSLSRKLTFLFLSITPLLADNYPTYVITDPTNQAAYTAQLPNL
                     THKIAAQYQLDNMNNIVRGNANAQAVATTGAGTAYAMGEHTFIVGASGAAAVATSDNT
                     FSQVFDKIQNLESGAVPQFGIGAALSAMVGMNLGNLRTPRYLGPFEIARTTLFVNAMN
                     YSQKAWTLQHRCWAHTSNTKCESRKEKVISSTVKFFSPPALITHTFLQNIIRPPAIKI
                     LYP"
     gene            768..1196
                     /locus_tag="PLY93_12440"
     CDS             768..1196
                     /locus_tag="PLY93_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70334.1"
                     /translation="MTVGTGTPADPLLIWTPQGTLNITSGSGTLPIEVSTNIRLFYFL
                     SFYAGGAVDLNVGKATTAIHLTADMKGTTSGGTPQSAGNSRLDSVIESSPQFASARAF
                     VGLQLNLLPATQGNFVALFTQVSASSIGGLAALVGVRGAW"
     gene            complement(1405..1728)
                     /locus_tag="PLY93_12445"
     CDS             complement(1405..1728)
                     /locus_tag="PLY93_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70335.1"
                     /translation="MPLKIITTSLSALFFFGACTAVKYNAHVSPDGSNGFLRTYYSSE
                     KEKAGGCSDGTGIEQCKIQGADVICKDLNIILPVEKVEESKPVATAETKPDVNKVNKA
                     KEKGK"
     gene            complement(1719..1928)
                     /locus_tag="PLY93_12450"
     CDS             complement(1719..1928)
                     /locus_tag="PLY93_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70336.1"
                     /translation="MKKIYLLLSLALVAFMGCRSIEITNFDVLSNKDGKVVYRVDAVE
                     NTTFSSRPLLGIICASDSKGDLVCR"
     gene            complement(1925..2227)
                     /locus_tag="PLY93_12455"
     CDS             complement(1925..2227)
                     /locus_tag="PLY93_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70337.1"
                     /translation="MKSGVMNFMRISFVAALALMAAHCATPAHYQYQRTVPGADGTVY
                     VQTLKIQPRIIYNSTKPVILNCKLRGENLNCDRELQINIDSTYWQAKDYFQKEKTP"
BASE COUNT          591 a          574 c          505 g          662 t
ORIGIN      
        1 aagcgaccag caaaattggc tgcatagctg tcaccgatgt ctcggggttc tacatattta
       61 gaatacaagt ttacgaatga ttcgtaggtt ttgatgagga caacaatgaa aagtctatcg
      121 cgtaaactta cttttctttt tttatcgatt actccccttc tcgcggataa ctatcccacg
      181 tacgtcatta ccgacccaac caatcaagca gcttataccg cacagctccc taacctcact
      241 cacaaaattg ccgcgcaata tcaactcgac aacatgaata acatcgtgcg cggcaatgcg
      301 aatgcacagg ctgtagcaac taccggtgct ggcactgcat acgctatggg tgagcatacc
      361 tttattgtcg gcgcaagtgg cgcagcggcg gtggccacaa gcgacaacac cttctcccaa
      421 gtttttgata agatacaaaa tttggaaagc ggcgcagtgc ctcaatttgg tatcggtgcc
      481 gcgctctcgg cgatggtggg tatgaactta ggtaatttgc gtactccccg ttatcttggc
      541 ccctttgaaa ttgcacgcac aacgctcttc gtcaacgcga tgaactactc gcaaaaggct
      601 tggacacttc agcatcggtg ttgggcgcac acgtccaata ccaaatgcga aagccgcaag
      661 gaaaaagtta tttccagtac ggtgaaattc ttttcaccac cggctttgat tactcacact
      721 tttctgcaaa atataattcg gccaccggcg ataaaaattt tgtacccgtg acggtgggca
      781 caggaacccc agcagacccg ctactcattt ggactcccca aggtacgctc aatatcacct
      841 caggttcggg cacgttaccc atcgaggttt caacaaacat acgtcttttt tattttctat
      901 ctttttacgc aggcggcgca gtcgatttga atgtaggcaa agccaccacc gccatccatt
      961 tgaccgcaga catgaaaggc actacatcgg gaggcacacc ccaaagtgca ggcaatagcc
     1021 ggttagacag cgtcatcgaa tcttcacccc agtttgccag cgcacgcgct tttgtgggac
     1081 tgcaactcaa tcttctgccg gcaacgcagg ggaattttgt cgctcttttc acccaggtga
     1141 gtgcctcctc aatcggtgga ctcgctgcac tcgtaggcgt gcgcggcgcg tggtgatttg
     1201 tccccttttc ttaaattaag ccattatcac aattttgggg ataactatcc cgtattatgt
     1261 cacattgggg gataactatc tcatgttatg tcacattagg ggataactat ctcgcgttat
     1321 gtcgcattag ggaataacta tctcgtatta tgtcgcatca tattgactgc taacatagcc
     1381 aagagtcatc cactgaattt atggctattt ccccttttct tttgctttat tcaccttatt
     1441 tacatccggt tttgtttctg ccgtagctac tggctttgac tcttccactt tttctaccgg
     1501 taaaataata ttcaaatctt tacagatgac atcggcgcct tggattttgc attgttcgat
     1561 accggtgcca tcgctgcacc cacccgcctt ttctttttcc gacgaataat acgtgcgtaa
     1621 aaagccattg gaaccatcgg gggaaacgtg tgcgttatat tttacagcag tacaagcacc
     1681 gaaaaaaaat aatgccgata gcgatgtggt tataattttt agcggcatac gagatcccct
     1741 ttgctatcgg aagcgcaaat gatgcctaag agtgggcgcg atgaaaaagt tgtgttttct
     1801 accgcatcga cacggtagac cactttgccg tctttattgg agagaacatc aaaatttgtg
     1861 atttcaatgc tgcggcagcc catgaaggca actaacgcca agcttagtaa caaataaatt
     1921 tttttcatgg tgttttctcc ttttgaaaat aatccttggc ctgccagtaa gtagaatcga
     1981 tattaatctg tagctcgcga tcgcagttta agttttcgcc acgtaatttg cagttgagta
     2041 tcaccggttt tgttgaatta tagataatac gcggctgaat ttttagcgtt tgtacataca
     2101 ctgtaccatc cgcgcctggg actgtgcgtt ggtattggta gtgtgccggt gtcgcacaat
     2161 gcgccgccat gagtgcaaga gcggcaacaa aagaaatccg cataaagttc ataacgccac
     2221 ttttcatttt gcacctgctt tgccgagtac ttctataatt tcggtatggt ttttacggcg
     2281 tgccgcttcg agcgcggtaa aagaatccca tgttttgata ttcacatcgg ct
//
LOCUS       DAOMPD010000431         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18233_length_2327_cov_2.501761, whole genome shotgun sequence.
ACCESSION   DAOMPD010000431 DAOMPD010000000
VERSION     DAOMPD010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18233_length_2327_cov_2.501761"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLY93_12460"
     CDS             complement(<1..298)
                     /locus_tag="PLY93_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRP70338.1"
                     /translation="MNSTIQVGILGAGGFTGQELMRIFARHPYVHLAYVTSSEYAGKT
                     ILEAFPALYHERYKNLKFSTHPQSVSDVPKLDVIFLATPDDVSLKWAPEFLNAGV"
     gene            complement(295..1161)
                     /locus_tag="PLY93_12465"
     CDS             complement(295..1161)
                     /locus_tag="PLY93_12465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRP70339.1"
                     /translation="MKIKTTGIILLFTMGFASACDKTPKTKAEAMKEVKEKMRDASNP
                     IAVMDTTEGTITIELYRHAAPKTVANFIDLAKGEKEFTDATGNKIKRPYYNGLIFHRV
                     IPNFMIQGGDIKGDGTGGPGYQFADEINAKALGLDKIKVVSSPMAQNEVMQVVRDAIF
                     KKLNIKSQADLNAKMAQANQMLQGEQSKWKDKSLEELYTARGITYTPNLASESNTKGS
                     LSMANAGPNTNGSQFFISVADNTYLDGKHTVFGRVLGGLDIAEKITKVERDQSDRPKK
                     PIKIEKLTILEN"
     gene            complement(1199..1945)
                     /locus_tag="PLY93_12470"
     CDS             complement(1199..1945)
                     /locus_tag="PLY93_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRP70340.1"
                     /translation="MKLKLKIWRQNDQKTKGAFKNYQIDNVNEHMSFLEMLDVLNEDL
                     THKGEEPVAFSHDCREGICGSCAMVINGHPHGPEHGTATCQLHMRKFNGQSVTVEPLR
                     AKAFPVLKDLVVDRSAFDRVQQAGGYITVSTGSAPEANLTPVPKATADEAFLAAQCIG
                     CGACVAACKNASAMLFVAAKVSHLALMPQGDAERADRVRKMVKAMDDEGFGNCTNTGA
                     CEAECPKEISISHIARMNREYFTASIINEN"
BASE COUNT          536 a          522 c          582 g          687 t
ORIGIN      
        1 taacacccgc attcaaaaat tcgggcgccc atttaagcga tacatcgtcg ggggtggcga
       61 ggaaaattac gtccagtttt ggaacatcgc tcacgctttg gggatgcgtt gaaaatttta
      121 agtttttgta ccgctcgtgg tataacgcgg ggaaagcttc caaaattgtt ttgcctgcgt
      181 attcagaaga ggtgacgtat gcgagatgga catacggatg tcgagcaaaa atacgcatga
      241 gctcttggcc ggtaaatcca ccggctccca aaataccgac ttgaatggtt gaattcaatt
      301 ttctaaaata gtgagctttt caatcttgat aggcttcttg gggcggtcgg actggtcacg
      361 ctctactttt gtaatttttt cagcaatatc taacccgccg agcacacggc caaaaacagt
      421 atgcttgccg tcaaggtacg tgttgtctgc gacgctgatg aaaaactggc taccgttggt
      481 gttggggcct gcgttagcca tcgaaagcga accttttgtg ttcgactctg aagctaaatt
      541 gggggtgtac gtgatgcctc gcgcagtata aagctcttcg agagatttat ctttccactt
      601 gctctgttcg ccctgtaaca tttgattcgc ctgcgccatt tttgcgttta aatcggcttg
      661 gctttttatg ttaagttttt tgaaaattgc atcgcgcacc acttgcatca cttcgttctg
      721 cgccatgggt gacgaaacca ctttaatttt atcgaggccc aacgcttttg cgttaatctc
      781 gtcggcaaac tgatagcccg ggccaccggt accatcgcct ttgatgtcgc cgccttgtat
      841 catgaagttg ggaattacac ggtgaaaaat taagccgttg tagtacggac gcttaatttt
      901 gttaccggtg gcgtcggtga attctttttc accttttgct aaatcgataa aatttgcgac
      961 ggttttgggt gcggcatggc gataaagctc gatggtaatc gtgccctctg tggtatccat
     1021 gacggcgatg gggttagatg catcgcgcat tttttcttta acttctttca ttgcttccgc
     1081 cttagttttg ggtgttttat cgcatgccga agcaaaaccc atggtgaaga gtaggataat
     1141 ccccgtagtt ttaattttca taattgtttt ttaatactcg ttttgtcgat tgaattattt
     1201 agttctcgtt aattatcgac gcggtgaaat attcacggtt catccgtgca atatggctga
     1261 tcgagatttc ttttggacac tccgcttcgc atgcaccggt gttggtgcag ttgccgaacc
     1321 cttcgtcgtc catcgctttt accattttac gtacacggtc ggcgcgttcg gcgtcgcctt
     1381 gtggcatgag agcaaggtgt gaaactttgg cagcgacaaa aagcatcgcc gaagcgtttt
     1441 tacacgccgc cacgcaagcg ccgcaaccga tgcactgcgc cgccaagaat gcttcgtcgg
     1501 cagtcgcttt aggcactggc gttaaattgg cctcgggggc tgaaccggtg ctgacggtga
     1561 tgtacccacc cgcttgttga acgcggtcga atgccgagcg gtcaacgact aaatctttga
     1621 gaacggggaa agctttggca cgtaaaggct ctacagtcac cgattggccg ttgaatttac
     1681 gcatgtggag ttggcaagtg gcggtgccat gctccgggcc atgcgggtgt ccgttgatga
     1741 ccatggcgca cgaaccacaa attccttcgc ggcaatcgtg gctaaacgcc accggttctt
     1801 cgcctttgtg tgttaaatct tcgttgagga catcgagcat ttcgaggaac gacatgtgct
     1861 cgttcacgtt atcgatttga taattcttga atgccccttt agtcttttga tcgttttggc
     1921 gccagatttt tagtttaagc ttcattatag actatggtgt gttttggaat tatggcataa
     1981 agcgaaaaac ggttttatac gtgaaattat gcggtatttt attgttgata tagtaaggat
     2041 tccttacatt atgcccatgg ttgaagcgct tacatccgac tctactttcg atagcaccgt
     2101 gacgagtaaa ggccaagtga cgctgcccgt gcatttcgta tccgcaaggc cgataaaaca
     2161 gacgtagcgt gggcgcgttc gatcgagtcc acgttaaacg aatggcaagg taacgcggac
     2221 gatgatctgt aatccgttcg atatcgttga ggttcctttt ctgttcagcg atcgtaaaga
     2281 agtaaaaaaa cgcaaagcat taattctatc cacgcggaag tataacg
//
LOCUS       DAOMPD010000432         2326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18243_length_2326_cov_2.264201, whole genome shotgun sequence.
ACCESSION   DAOMPD010000432 DAOMPD010000000
VERSION     DAOMPD010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18243_length_2326_cov_2.264201"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLY93_12475"
     CDS             <1..293
                     /locus_tag="PLY93_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70341.1"
                     /translation="VGSMFFYYAGAGLTYPVAVAQSITLTPFATVGFSGGKLDYQSGY
                     TFLLPALDVGASAEWALSERLRLAAALSYRHVFDPTLPGSFIQLHLGAGYIF"
     gene            317..>2326
                     /locus_tag="PLY93_12480"
     CDS             317..>2326
                     /locus_tag="PLY93_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70342.1"
                     /translation="MKRKLMAIVMAATILGCSQYGDKMPQQKDAAAVTSSSKDITDFS
                     ILGVTGTITGNAVSLTVPYGTNVTALVATFTTTGASINIAGTDQVSGTTANNFSTPQT
                     YTVVAADGSTKNYTVTVTVALATSKDITAFSILGASGTITGSSISVTVPYGTNVTALV
                     ATFTTTGASVNISGANQVSGTTANNFSTAKTYTVVAADSSTKNYTVTVTVALNTAKDI
                     STFSISGISGTITGNTIAVTVPYGTNVTALVATFTITGASVNISGTSQVSGTTANNFA
                     TAKTYTVVAADGSTKNYTVTVTVNPTPPVAAQWARSVTSGSDDSRFQAVAVDSAGNIY
                     AAGYQYGTGSYTYGSGVSVAGTYNGNNIVLVKYNSAGTAQWARSITSGSGESQFNAVA
                     VDSSGNIYAAGYQKGSGSYTYGSGVSVAGSASGTYYNVVLLKYNSSGTAQWARSVTSS
                     SYTSRFNAVAVDSSGNIYAAGAQPGTGSSYGSGVFVKGSYGGTNIVLVKYNSAGTAQW
                     VRSATDGSDSSEFNAIAVDSSGNIYAAGTQVGTGSNTYDTGVSVAGSGGMNIVLVKYN
                     SAGTAKWARSVTSGGAQSEFYAVAADSAGNIYAAGRQNGNASYTYGSGVSVAGGASDN
                     IVLVKYNSSGTAQWARSVPSGSTSWFNSVAIDSSGNIYAAGYQYGT"
BASE COUNT          579 a          589 c          641 g          517 t
ORIGIN      
        1 atgtaggcag tatgttcttt tattacgcgg gcgcggggct cacctacccc gtcgcggtgg
       61 cgcaaagcat cacgcttacg ccatttgcaa cggtgggatt ttcaggcggc aagctcgact
      121 accaaagtgg ctacacgttt ttactgcccg cgctcgacgt aggcgcctct gccgaatggg
      181 cgctgagcga acgcctacgt ttggcggcag cgttgtcgta ccggcatgtt ttcgacccaa
      241 cgctaccggg gtcttttatt caactgcatt taggggcagg ctatattttt taagaggtaa
      301 attaaaaggg ggatatatga aacgaaaact tatggcaatt gttatggcgg caaccatttt
      361 aggttgtagc caatatgggg ataaaatgcc gcagcaaaaa gatgcggcag cggtaacgag
      421 ttcatccaaa gacatcaccg attttagtat tttaggcgta acgggcacca ttaccggcaa
      481 cgcggtgagt ctaaccgtac cctatggcac aaatgtcacc gcgttggtgg ccacgttcac
      541 caccaccggc gcaagcataa acatcgccgg cacagaccaa gtgagcggca cgacggcgaa
      601 taacttttca acgccccaaa cctataccgt agtggcagcg gatggctcta caaaaaatta
      661 tactgtgaca gtaacggtag cattagcgac ttctaaagac atcactgcgt ttagtatttt
      721 aggtgcttca ggcacaatta ccggtagctc gattagtgtt accgtaccct atggcacgaa
      781 cgtcaccgcg ttggtggcca cgttcaccac cactggtgca agcgtcaaca tcagcggcgc
      841 aaaccaagtt agtggcacga ctgcgaataa tttttcaaca gctaagactt acaccgtcgt
      901 ggcggcggat agctcgacga agaattatac cgtgacggtc acggtggcac tgaatacagc
      961 taaagacatc tcgacgttta gtatttcagg tatatcaggc acgattaccg gcaacaccat
     1021 cgccgttacc gtaccctacg gcaccaacgt caccgcgttg gtggccacgt tcaccatcac
     1081 cggtgcaagc gtcaacatca gcggcacaag ccaagtgagc gggaccacag cgaataattt
     1141 tgcaacagcc aagacttaca ccgtcgtggc ggcggatggc tcaacgaaaa attatacggt
     1201 gacggtgacg gtaaacccga ccccaccggt tgcagctcag tgggcacgtt cggtgaccag
     1261 tggcagtgac gattcgcggt ttcaagccgt tgccgtcgat agcgcgggca atatctacgc
     1321 ggcgggttat caatacggaa ccggtagcta tacatacggc agcggagtat ctgtggcggg
     1381 cacttacaac ggcaataaca tcgttctagt gaaatacaac tcggctggca cagcgcaatg
     1441 ggcgcgatcg attaccagtg gaagtggcga atcgcaattt aatgccgttg ccgtcgatag
     1501 ctcgggcaat atctacgcag caggttatca aaaaggtagt ggtagctata cttatggcag
     1561 tggggtatcc gtggcgggta gcgcgagtgg cacctattat aacgtcgttc tattaaaata
     1621 caactcatct ggtactgccc aatgggcgcg ctcggttacc agtagcagtt atacatctcg
     1681 atttaatgcc gttgccgtcg atagctcggg caatatctac gcggcgggtg ctcaacctgg
     1741 aaccggtagt agctatggta gcggggtatt cgtgaagggg agttatggcg gcaccaacat
     1801 cgttctcgtg aaatacaact cggctggtac agcgcaatgg gtccgatcgg cgaccgatgg
     1861 aagtgacagt tcggagttta atgccattgc tgtcgatagc tcgggcaaca tctacgcggc
     1921 ggggactcaa gttggaactg ggagcaatac atacgacaca ggcgtgtcgg tcgcgggcag
     1981 tggtggcatg aacatcgttc tagtgaaata caactctgcc ggtacagcga aatgggcgcg
     2041 ctcggttacc agtggcggtg cccagtcgga attttatgcc gttgccgccg atagcgcagg
     2101 caatatctac gcagcaggac gtcaaaacgg aaacgctagc tatacatacg gcagcggagt
     2161 atctgttgca ggtggtgcta gcgacaacat cgttttggtg aaatacaact cttctggcac
     2221 agcacagtgg gcgcgctccg ttcccagtgg ctccacttcg tggtttaact ccgttgccat
     2281 cgatagctct ggcaatatct acgcggcggg ttatcaatac ggaacc
//
LOCUS       DAOMPD010000433         2322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18281_length_2322_cov_3.363917, whole genome shotgun sequence.
ACCESSION   DAOMPD010000433 DAOMPD010000000
VERSION     DAOMPD010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18281_length_2322_cov_3.363917"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..142)
                     /locus_tag="PLY93_12485"
     tRNA            complement(68..142)
                     /locus_tag="PLY93_12485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Arg,seq:acg)
     gene            297..1148
                     /locus_tag="PLY93_12490"
     CDS             297..1148
                     /locus_tag="PLY93_12490"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRP70343.1"
                     /translation="MQNTPFVLKNHKDGFELYAQTWKPESYSKVMVIQHGFGEHSNRY
                     KNLLQFAEKNNTAVYALDARGHGKTPGKRGHVDDFNLYANDLVLLIEKATTENANKPI
                     FLLGHSMGALVAALATLQGDTAKKLKGLVLSSGGFLPVLDTVKAIKKTAASLLANFVP
                     AAVVDAGLDIRLLSRDEQSVKEYQSDPLVHGKISFRMGSDLFRYGQELIELAPRFTLP
                     LYVFHGDADGIARVEGSKAFFNAAGSQDKTLKIYPGFYHETMNEPFVDRQTVLTNLFD
                     WINAHAK"
     gene            complement(1141..>2322)
                     /locus_tag="PLY93_12495"
     CDS             complement(1141..>2322)
                     /locus_tag="PLY93_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRP70344.1"
                     /translation="QAIPTYFIFVVLLNLHIKYFWIVLIGALTAFFLNVYSATYVAEN
                     HKTARGLIVAAASSVSTVVHRGIAVGMRGSAIPALIIVTMMGIVYMLGIVEEKGENKF
                     VHGMYALSLALTSMLSLFAVSQATAAMMPLASSAVNRLKMNASQDEKKVLLTKFRALR
                     SVSVPSFVLHGKVMLSALVVLAFLVYAQILSSAGQASLFKHLMQIAMLLVGGIASYFL
                     SARVNELVLNLGPRLVRTTRQTFRETGYAGATTTTTDMGTLWQTANRYLMRKTLPLFI
                     GTILLPALTCIVGGAHGLTGYLIGFGFLSFLNGNSWLTTGAAWSSARHAAEADMYVTR
                     DTAQFNALVEADMVGDSMHEAVAPILSGGILVTIIASLMFTPATLVLHERLREIVLRF
                     I"
BASE COUNT          680 a          518 c          534 g          590 t
ORIGIN      
        1 cacgcagatg caagacgcct ttagcagttt tgtcatcgca tgatggagaa gcttttagga
       61 gcctatgcgc gcccgagagg agtcgaacct ctgaccctca gattcgtagt ctgatactct
      121 aatccagctg agctacgggc gctttaagtt ttgcctaaaa agcttctttt gataggctgt
      181 caacttttag agtagcttca tttgtaaagc taaatcttgg cgcacaggct ctttagacac
      241 tccacaggaa ttatgaattg cgtttgtcgc accgtttcat cacttttgat tgtcccatgc
      301 agaatacccc attcgtctta aaaaaccaca aagacggttt tgagctttat gcacaaacgt
      361 ggaaaccaga gagctattca aaagtcatgg ttattcaaca tggttttgga gagcattcaa
      421 accgatacaa aaatctttta caatttgcag agaaaaataa cacagctgtt tacgcactcg
      481 acgcgcgtgg ccacggtaaa acgccaggta aacgggggca tgtcgacgat tttaatttat
      541 acgccaacga cttagtgtta ctcatcgaga aagctaccac cgaaaacgcc aataaaccca
      601 tttttttact cggccattcg atgggagcgt tggtcgcggc actagcgaca ctgcaaggag
      661 ataccgcaaa gaaactgaaa gggcttgttt tatccagcgg tggattttta ccggtgttgg
      721 atacggttaa agcaataaaa aagactgccg catcgttgct tgctaatttt gtaccagcgg
      781 cagtcgttga cgcaggttta gatatacgcc ttctgtcgcg cgatgaacaa agcgtgaagg
      841 agtaccaaag cgatccttta gtacacggta aaatatcgtt tcgtatgggc agcgatcttt
      901 ttcgttatgg ccaagaactg attgaattag cgccacgttt tactttaccg ctttatgttt
      961 ttcacggtga tgcagacggc attgctcgtg tcgaaggatc aaaagcattc tttaatgcag
     1021 ccggttcaca agataaaaca ctaaaaattt atcctggatt ttatcacgaa acaatgaatg
     1081 aaccttttgt tgatagacaa acggtattga cgaatttatt cgattggatt aacgcacacg
     1141 ctaaataaag cgtaatacaa tttcacgtaa acgttcgtgt agcacaagcg tcgccggagt
     1201 aaacatgagt gatgcgataa tcgttactaa aattcccccc gaaagtatgg gcgcaaccgc
     1261 ttcgtgcatc gaatcgccca ccatatcggc ttcaacgagc gcgttaaact gtgctgtatc
     1321 ccgcgtgacg tacatgtctg cttcggcggc atgtcgtgca ctcgaccaag ccgctcccgt
     1381 ggtaagccaa ctgttaccgt tcaaaaaaga aagaaagcca aaaccgataa gatagccggt
     1441 taacccgtgc gcaccgccaa cgatacaggt tagcgcgggt aataaaattg tacctataaa
     1501 aagggggagt gtcttacgca ttaagtaacg gttggccgtt tgccaaagcg tacccatatc
     1561 ggtagtcgtt gttgtcgcac cggcatagcc ggtttcacga aatgtttgcc gagttgtacg
     1621 taccaaacgt ggacctaagt ttagcaccag ctcgttgaca cgagcggata aaaaataaga
     1681 agcgatgcct cctactaaaa gcatagcgat ttgcattaag tgtttgaaaa gcgaggcttg
     1741 gcctgcgctt gaaagaattt gtgcgtacac caagaacgcc agaacgacaa gtgctgatag
     1801 cataaccttg ccgtgcaaca cgaacgaagg cacagagaca ctacgtaatg cgcgaaattt
     1861 ggtgagtagt actttttttt cgtcttgtga agcattcatt tttagacggt tgactgcaga
     1921 cgacgcaagg ggcatcatcg cagctgtcgc ttgagagact gcaaaaagag aaagcatcga
     1981 agtgagcgcg agcgatagag cgtacatacc gtgtacgaat ttattttctc ctttctcttc
     2041 aacgataccc agcatataaa ctatgcccat cattgtaacg ataatgagag ccggtattgc
     2101 cgaacctcgc atgccaacag cgatgcctcg gtgtaccact gttgaaacag agctcgctgc
     2161 cgcgacgatg agcccacgag cggttttatg attttctgct acataagtag cggagtaaac
     2221 attcaagaaa aatgcagtta acgcccctat aagaacaatc caaaaatatt tgatgtgaag
     2281 gtttaggagc acaacaaaaa taaagtaagt aggtatcgct tg
//
LOCUS       DAOMPD010000434         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18337_length_2317_cov_2.030946, whole genome shotgun sequence.
ACCESSION   DAOMPD010000434 DAOMPD010000000
VERSION     DAOMPD010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18337_length_2317_cov_2.030946"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLY93_12500"
     CDS             complement(<1..1060)
                     /locus_tag="PLY93_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70345.1"
                     /translation="MRKSNIYTVTILFLSVFLFSFACKSASQKDSTADGWVTVTGVAQ
                     IANGNMGLARDEALKDAKRVAVQEVLGSMVSARSDVVNYELVRQSVTAKSEGMIEEFQ
                     ILSAGASSQNEYQVKIKAKVSERMVNDTISEVIASQGKPRIMALITETLDGKNDANQV
                     AQTEIESQFTAQNFPFVDKATVEKILAKNRKKIAAALGGNNDAAREVGVDAGAEIILV
                     GASRVKSAGKIANSQLVSMQADVALRVIDVNTGKILTAGQQHGAFPHINPETGAVEAV
                     KRAVTPLREKLVADIVRLWDINRAHTITLLVVGLDYERLMVLRGELTEKVRGVTAVNL
                     KGSSGKAATLEIEFQGSST"
     assembly_gap    1134..1143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1144..2244)
                     /locus_tag="PLY93_12505"
     CDS             complement(<1144..2244)
                     /locus_tag="PLY93_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70346.1"
                     /translation="MADSIKTSISDSAKAFLLSDREGEFQGENFERAFIRYYLALNQV
                     LQGDLAAAKRTLRKLDADLKDMKYDDVAYKQILIARYLDALVSEELKEYNDARVQYKN
                     LENFGVDTAWLNRERYTLAARAKDRADMAKYVSFKPDTLLPSSEDPTSEKIGELVLIC
                     ETGKAAVKESRGKLLTDEQFMIPLRISIDIAVLSDGKGMSTAGIIAMMGTAENPIPRF
                     VERDALPLPSIQIGGAAIPGFRELTNFDNMAQKNFNDNYSSYINKNVASIATKIVLAA
                     VAAEAVSSAIRKNTNDNLIGALGGLAVGIGAGAGVAATVKPDLRSWHTLPTGFSATRL
                     FLPAGKYPLQVDGDGEAVIVYGKPALEVEIQPH"
BASE COUNT          498 a          583 c          581 g          645 t
ORIGIN      
        1 cggtcgacga accttggaat tcgatttcta acgttgctgc tttccccgaa gaacctttca
       61 aattcactgc ggtaacgccg cgcactttct ctgtaagttc tccccgcaaa accatcaagc
      121 gttcgtagtc gagccccact accaaaaggg taatggtgtg ggctcggttt atatcccaca
      181 gacgaacaat atctgctacc aacttttcgc gcagcggtgt caccgcgcgc ttcacggctt
      241 ccaccgcacc cgtttcagga ttgatgtgag ggaaggcgcc atgctgttga cctgctgtta
      301 aaattttgcc ggtgttcacg tcgatgacgc gcagtgcaac gtcggcttgc atcgaaacaa
      361 gttgtgaatt cgcgattttg cccgcagatt taacgcggct ggcgccgacc aaaataattt
      421 cagcgccggc gtcgacgccc acttcgcgcg cagcgtcgtt atttcctcct agtgcggctg
      481 caattttctt tcgattctta gccagaattt tttcgacagt tgctttatcg acgaacggaa
      541 aattttgtgc ggtaaactgg gattcaattt cggtctgtgc gacttgattg gcgtcgtttt
      601 taccgtctaa tgtttcggtg atgagcgcca ttatgcgcgg tttgccttgc gatgcaatca
      661 cttcgctaat ggtgtcgttc accatgcgct cgctcacttt tgctttaatt ttgacttgat
      721 actcgttttg cgacgatgca cccgctgaaa ggatttgaaa ttcttcgatc ataccttcac
      781 tttttgcggt gaccgattgc cgcacgagct cgtaattcac cacgtcgctg cgcgcagaaa
      841 ccatgctacc caatacttct tgcaccgcta cgcgtttggc gtctttgagc gcttcgtcgc
      901 gcgcaaggcc catgttacca ttggcaattt gtgcaacacc ggtgacagtc acccaaccgt
      961 cggcggtact gtctttttgt gatgcgcttt tgcacgcaaa ggaaaataaa aataccgata
     1021 aaaataaaat tgtgacagtg tagatgttgg atttacgcat ggtgttagtc tccttagtgt
     1081 atttcatatc ccgtcaagag tgttattgtt ttttgaaaag ccgcaaggcg acannnnnnn
     1141 nnngtggggt tggatttcca cttcaagcgc gggcttaccg taaacgatga cggcctcacc
     1201 gtcgccgtcg acttgtaacg ggtatttacc tgcgggtaag aaaagtcgcg ttgcgctaaa
     1261 acccgtaggt aaagtgtgcc agcttctcag gtcaggtttg accgttgctg ccacgcccgc
     1321 ccccgcgccg atgcctacgg caaggccgcc caaagcaccg atgagattgt cgttggtatt
     1381 cttgcggatg gcgctcgata ctgcctcggc ggcaacagcg gcgagcacaa tttttgtggc
     1441 aatcgacgcg acgttcttat tgatgtacga cgaataattg tcgttgaagt ttttttgcgc
     1501 catgttgtca aaattggtga gctcgcgaaa accagggatc gccgctccac caatttggat
     1561 agagggaagc ggtagtgcat cgcgctcaac gaagcggggg attggatttt ccgcggtgcc
     1621 catcatcgct ataatccccg ccgtcgacat tcctttgcca tcgctcaata cggcgatatc
     1681 aatcgagatg cgcagtggaa tcataaattg ttcgtcggtc aacaatttgc ctcggctttc
     1741 tttcaccgcc gctttaccgg tttcgcaaat gagcaccagt tcgcctattt tttcggaagt
     1801 gggatcttca cttgagggca ataaagtatc gggtttaaaa gacacatact ttgccatgtc
     1861 ggcacggtct ttcgcgcgtg cagctaaagt gtagcgttcg cggttgagcc aagcggtgtc
     1921 gacgccaaag ttctctaaat ttttatattg cactcgggcg tcgttgtatt cctttaattc
     1981 ttcagaaacc aatgcgtcga ggtagcgggc gataagaatt tgtttgtacg ccacatcgtc
     2041 gtacttcatg tctttgaggt cggcgtcgag tttgcgcagg gtgcgttttg ccgccgccaa
     2101 atcgccttgc aaaacttgat tgagcgcaag gtagtaacgg ataaatgcgc gttcaaaatt
     2161 ttcgccttgg aactcgcctt cgcggtcgct caaaagaaac gcttttgcag aatcgctgat
     2221 gctggttttg atggagtcgg ccatttccgc tgcgcgggaa aaaatttcat tgctggtgga
     2281 gagatctcct tttgcgcgat agataatacc tgcttcc
//
LOCUS       DAOMPD010000435         2310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18412_length_2310_cov_2.670510, whole genome shotgun sequence.
ACCESSION   DAOMPD010000435 DAOMPD010000000
VERSION     DAOMPD010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18412_length_2310_cov_2.670510"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLY93_12510"
     CDS             <1..483
                     /locus_tag="PLY93_12510"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRP70347.1"
                     /translation="KRLDFVNVFTAMRPMRKIAVALLFFTSFLNAKNITDYHKKQFDK
                     VTFNRYGIAHIVTPQGWVAINKKNKVLYHPYIYDNGPDYVKEGLLRFVENGKMGFVDE
                     RGKKIIHAQFDFVAPFESGKAKYCIGCQKIQDGEHSRIDEKTGTWGYIDKKGKRISIN
                     "
     gene            516..>2310
                     /locus_tag="PLY93_12515"
     CDS             516..>2310
                     /locus_tag="PLY93_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRP70348.1"
                     /translation="MNHPLFTLPLKNPIFIFSLILFIILLVPFFLRRLHIPYVVGLIL
                     AGVLIGPHGTHLIERDASIVLFGTVGILYLMFLAGLEVDIADFIKQRNRSLIFGLYTF
                     STSLILGTLMGRYALSMPWLGSILLGSLLSSHTLLTYPIVGRFGVTQNKAVIVTIGGT
                     VIADTLALLTMAIIVSMTHGTINTRFWTSIGISIIAFFAFVLFAIPPFVRWVYKRIED
                     EISQYIFTLGIVFFCAFLAEAAGIDGIIGAFLSGLTLNQLIPRTSPLMNRIHFVGNAI
                     FIPFFLIGVGMLVNLKILFLSPKAIVIAVGLSALAIFSKYIAAVFAQKTFRFSPEQRS
                     LIFGLSSARAAATLAIVLIGHNVILGTDTNGNSVRLLDDNILNGSILMILLTSTVAAF
                     ATERAARKIAEKENVEPTLDEKNTRVLMIALSETNAAKDLIDLAAAMVNRKAGDFVKG
                     IHIINETTDSSDTIKHSRKLLEQTEKYAAGTGFELPCDLRLDLNITTGLIHSVKENRA
                     EELLTGFHKKTFLSRSALGHVIESYLERSSDIVYMYRSIQPLGTYKRLVVAIPPHAEK
                     NHGFSRWFRRLRTLAVSAGMRTIFYCTINSQL"
BASE COUNT          632 a          496 c          461 g          721 t
ORIGIN      
        1 aagcgacttg actttgtgaa tgtctttacc gcaatgcgcc ctatgcgaaa aattgctgtc
       61 gctctcctct ttttcacaag ctttctcaat gcaaaaaata taaccgacta tcacaaaaaa
      121 caattcgaca aggtaacttt taatcgatat ggcattgcgc atatcgtcac tccccaaggt
      181 tgggtagcaa tcaacaaaaa aaataaagtg ttgtatcatc cttatatata cgataacggc
      241 ccagactatg ttaaagaagg gttgttgcgc tttgtggaaa atggtaaaat gggtttcgtg
      301 gatgaacgtg gcaaaaaaat tatccatgca caatttgatt ttgtcgcccc ttttgaatcg
      361 ggtaaagcca agtattgcat cgggtgccaa aaaattcaag acggcgaaca ttcgcgtata
      421 gacgaaaaaa caggaacctg gggctatatc gacaaaaaag gtaaacgaat ctctatcaat
      481 taagagaata tcaaaacagt aataaattta cacagatgaa tcatcctctt tttacattac
      541 ctcttaaaaa tcccatcttt attttctctc ttatcctttt tattattttg ctcgtaccct
      601 tttttttacg ccgcctacac ataccgtatg tcgtcggctt aattttggcg ggtgtactca
      661 tcggtcccca tggcacgcac cttatagaac gcgatgccag tattgtactc tttggtaccg
      721 tcggtatttt atacctcatg tttttagcgg gcctcgaagt ggatattgcc gattttatta
      781 aacaacgcaa ccgcagttta atatttggtc tctatacttt ttctacgtct cttattttag
      841 gcacacttat gggtaggtat gctctatcta tgccttggtt aggttcgatt cttttaggca
      901 gcttactctc gtcgcacacg cttttaacgt atccaatagt gggacgcttt ggcgtcacgc
      961 aaaataaagc ggtcatcgtt accatcgggg gcactgttat cgccgatact cttgcgctac
     1021 tcaccatggc gattattgta agtatgacac acggaactat caatactcga ttttggacaa
     1081 gtataggtat ttcaattatt gctttttttg cgtttgttct atttgcaata ccgccttttg
     1141 tgcgttgggt atataaacgt atagaagacg aaatatcgca atatattttt actttaggta
     1201 tagttttctt ttgtgcattt ttagctgagg ccgctggtat agacggtatc atcggcgcat
     1261 tcctttcagg gcttaccctc aatcaactca taccgcgcac ttcgcctctc atgaatagaa
     1321 ttcactttgt aggtaacgct attttcattc catttttcct tattggcgta ggcatgctgg
     1381 ttaatctcaa aattttattt ttgagtccaa aagccatcgt tatagcggtt ggtctttcgg
     1441 cattagcaat ttttagtaaa tatattgccg ccgtttttgc tcaaaaaaca tttcgtttct
     1501 ccccagaaca gcgcagtctt atctttgggt taagcagtgc acgcgctgca gcgactttgg
     1561 ctattgtttt aatcgggcac aacgttatat taggcaccga tacaaacggt aactctgtgc
     1621 gcttactcga cgataatata ctcaacggct ccatcttaat gattctgtta acaagtacag
     1681 ttgcggcgtt tgcaaccgaa cgtgcggctc gaaaaattgc cgaaaaagaa aacgtagagc
     1741 ctactctcga tgaaaaaaat acgcgcgtat tgatgatcgc tttatcggag acaaatgccg
     1801 ccaaggattt aatcgactta gccgctgcta tggtcaaccg caaagccggc gactttgtaa
     1861 agggaattca cattattaac gaaactaccg attcttcaga tacaattaag catagccgga
     1921 aattgttaga acaaaccgaa aaatacgcag cgggaacggg ctttgaatta ccttgtgact
     1981 tacgcttgga tttgaatata acaacggggt taattcattc cgtaaaagaa aatcgtgcag
     2041 aggaactctt gacgggtttt cacaaaaaga cgttcctttc gcgctccgct ttggggcatg
     2101 tgattgaaag ttatttagaa cgcagtagtg acattgtcta tatgtaccgt tctattcaac
     2161 ctttgggtac atataaaaga cttgttgttg caatccctcc acacgccgaa aagaaccatg
     2221 gtttctcgcg ttggttccgt cgtttgcgca cgttggccgt gagcgcgggc atgcgcacta
     2281 ttttttactg caccatcaat tcgcaactgg
//
LOCUS       DAOMPD010000436         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18450_length_2306_cov_3.253665, whole genome shotgun sequence.
ACCESSION   DAOMPD010000436 DAOMPD010000000
VERSION     DAOMPD010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18450_length_2306_cov_3.253665"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1167)
                     /locus_tag="PLY93_12520"
     CDS             complement(184..1167)
                     /locus_tag="PLY93_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRP70349.1"
                     /translation="MPVKCLIPAAGKGTRMRPLTHTLPKAMLPVAGKPTIFHIIDRAH
                     KAGVTDFVIITGYLCELMESEILEAYPHLNIEFVEQKEQLGLGHAIYTAKEKFLNKGE
                     SSPLSLFLIYGDTLFEGDIETMMQSKEPVIGVYEVEDPRRFGVIEKGEGQQIKSLVEK
                     PEKFVSNLAIPGVNFFPDAAKLFSALEYIITNDIRTKNEYQATDAFQYMLQTQQIPFV
                     WQRLTAWDDAGTLDAILETNKIILSHNGKGKEFAVAQDAKIIDSDISQFTSVSAGAVV
                     EKSTLVNCIVDRGSIIKNCKLENSLIGRNAVLENVAGVVVVGDDTQLHLKR"
     gene            complement(1446..1916)
                     /locus_tag="PLY93_12525"
     CDS             complement(1446..1916)
                     /locus_tag="PLY93_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRP70350.1"
                     /translation="MKPEKKPMPELEASTWLNSPPLTIEGERGKVVLLYAFQMLCPGC
                     VTHASPQVQKIRNFYDSDILTVIGLHTVFEHHSAMQEESLKAYLYEFKYTYPVAIDAP
                     SNDSPLPRTMTKLGMRGTPSILLIDKQGNLRRHFFGQMEDLYLGTEIGLLLAEG"
BASE COUNT          624 a          535 c          534 g          613 t
ORIGIN      
        1 ggtagcgttg gtggtgtaat ccacgtccgc taaacggcca tccaaaacca aatcgcgcac
       61 gctaccgccc acgagccacg cctcaaagcc atgctttacg agcgtctctt cgatgtagcg
      121 acagagtgaa tagtgcggtt ctggaatgaa gattttagat ggcacaactt tattgaagga
      181 gaatcaccgc ttgagatgta actgggtgtc gtcaccgaca acaaccacac ccgcgacgtt
      241 ttccaaaaca gcgttgcgcc caatgagtga attttccaac ttgcaatttt tgatgatcga
      301 cccccgatcg acaatgcagt tgacgagcgt tgatttttcc accacagccc ctgccgacac
      361 actggtgaac tgacttatat ccgaatcgat aatctttgca tcttgtgcaa cggcaaactc
      421 tttccccttg ccattgtgcg ataaaataat tttgttcgtc tccaaaatcg catcgagagt
      481 gccagcgtcg tcccacgcgg tgaggcgttg ccacacaaac ggtatttgtt gcgtttgcaa
      541 catgtactgg aatgcgtcgg tcgcttggta ttcatttttt gtgcggatgt cattcgtgat
      601 gatgtactcc aatgcagaga aaagttttgc tgcatcgggg aaaaaattca ccccggggat
      661 cgctaagtta gaaacaaatt tttctggctt ctcaaccaaa cttttaatct gctggccctc
      721 tcccttctca atgacgccaa agcggcgggg atcttccact tcgtaaacac caatgacagg
      781 ttccttcgat tgcatcatgg tttcaatgtc gccctcgaac aacgtgtctc cataaatcaa
      841 aaataaagat aacggggatg actccccttt atttaaaaac ttttctttcg cagtgtaaat
      901 cgcatgcccc aatcccaact gttctttttg ttcgacgaac tcgatattca ggtgagggta
      961 cgcctctaaa atttcgctct ccatgagttc acacaagtaa ccggtgatga taacaaaatc
     1021 agtcacgccc gctttgtgcg cgcggtcgat gatatggaaa atggtgggtt taccagcaac
     1081 ggggagcatc gctttgggga gcgtgtgcgt gagtgggcgc atgcgtgtgc ctttaccggc
     1141 agcggggatt aaacacttaa cgggcatggt gcattttcga gaagatagag agttgtgcaa
     1201 gctcttaatg gagaaagcac aagtttgttt ttgttggcgc acaggttcct aagtaaatgc
     1261 acgggacgtt gccgaagaaa atagcacgcg tatgctaatc aaagcgctcg ttgatgcagc
     1321 cacgcgacgt tgaattttaa gtcgccctaa aactgactga aattacaaaa tacagcctaa
     1381 aaccgtctct cctaaatggg agggtcaatg cttcttcgtg ccacgaaggc tatagacaca
     1441 aaaactcatc cctccgccaa aagcaatcca atctcggtgc ccaaatacaa atcctccatc
     1501 tgaccaaaaa agtgcctacg caaattgcct tgtttatcaa tgagtaaaat gctcggtgtg
     1561 ccgcgcatac ccaatttagt catcgtgcgg ggtaatgggc tgtcattgct tggtgcgtcg
     1621 atggcaacgg ggtatgtgta cttaaactcg tagagatatg ctttgagcga ctcttcttgc
     1681 atagctgagt ggtgttcaaa aacggtgtgt agtccaatca cggttagaat atcactgtcg
     1741 taaaaattac gaattttttg tacttgcggt gaagcgtgcg tcacacaccc gggacaaagc
     1801 atttgaaacg cataaaggag aacaaccttg ccacgctcgc cttcgattgt caacggaggt
     1861 gaattcaacc atgtactcgc ttcaagctct ggcatgggtt ttttctctgg tttcatggaa
     1921 atagtgtatg cttatgggcg ggaaagagcc acaaaaaatg gcgctttagc tgcaaaggaa
     1981 aacgagcttg atcactgtac aattttatgc gttagtgcca aattcgacac tctatccatc
     2041 gtccagacat cgtggggata actatcctca taagcaattt tagcaaaaaa atcacgattt
     2101 tttctaaaaa tctgtacttt tttttctcaa aagtgagaaa atttgagcgt tttctggtat
     2161 ttaatatata gagggcaata tgttaaagac atcgatcagc ttacccaaag acaccgtcgc
     2221 gacttggggc caaaaccact gggcgattat gcggatgtac gcacggtttt tgcgagtcgt
     2281 cgagcggaaa agaaagtttc ggcggg
//
LOCUS       DAOMPD010000437         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18499_length_2302_cov_2.234980, whole genome shotgun sequence.
ACCESSION   DAOMPD010000437 DAOMPD010000000
VERSION     DAOMPD010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18499_length_2302_cov_2.234980"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLY93_12530"
     CDS             <1..773
                     /locus_tag="PLY93_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70351.1"
                     /translation="IYALLCRAKILTRAMQQIFAKCVGLLLGFSIVLSALSMQQLQAQ
                     NAKPTIVELINLLKPGSLTHEKGKWVQTWVDAPGFDGVQPATVDIPNGFASFVDTGTG
                     AGSISHEFAVYYPEGAGAILVHNFQDDRDAYVSEIKFYQLRATKLQPVASPLTQPVCR
                     DLASAATIKTFYKNPLLSGLDPDPILPTFELPRKGTTILAYCSGNLNRFQVQQTMAGK
                     LDNNLDMQEKIHQSIQFTSKIQYLWDKKKGVFRSKKVK"
     gene            1339..1563
                     /locus_tag="PLY93_12535"
     CDS             1339..1563
                     /locus_tag="PLY93_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70352.1"
                     /translation="MREFVRLWHVAAERSTGKKIAKPRVMKVKSREFHAMENATRTGE
                     EEKLVVEPYWIMHFDGTSQFRNKERSVLKL"
     gene            1731..>2302
                     /locus_tag="PLY93_12540"
     CDS             1731..>2302
                     /locus_tag="PLY93_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP70353.1"
                     /translation="MLDNATVENLIARYDSPVPRYTSYPTALEFKPLEDAALNDSYQQ
                     CNPNHPFSVYIHLPFCENQCLYCGCNVLLSRRKDILDTYIPLLIQEIQSKTELLSFRP
                     KISQLHFGGGTPNFLRLPDWDKLFEALRKNFDFNDNAEISVELDPMYLGEGYLNHLRT
                     LGFNRVSYGVQDVTPKVLEAVNRPQDIDRIR"
BASE COUNT          636 a          541 c          521 g          604 t
ORIGIN      
        1 atatttatgc tttactttgt cgcgcaaaaa tactgactcg tgccatgcaa caaatttttg
       61 ctaaatgcgt gggtctcttg ctcggatttt ccatagtcct gtccgcactc agcatgcagc
      121 agctacaggc acagaacgcc aaacccacaa tcgttgagtt gataaatcta ttgaaacccg
      181 gctccctgac ccatgagaaa gggaagtggg tacagacctg ggtcgatgct cccggttttg
      241 acggcgtcca acctgcgacg gtggatattc ccaatggatt tgcgtccttt gttgataccg
      301 gaaccggagc ggggagcatc tcgcatgagt ttgccgttta ttatcccgaa ggggcaggcg
      361 ccattctggt acacaacttc caagacgatc gcgacgccta tgtgagtgaa ataaaatttt
      421 atcaactgcg cgcaacaaag ctgcaacctg tcgcatcgcc cttgactcaa cctgtctgcc
      481 gcgaccttgc ttcggccgcg accataaaaa ccttctacaa gaacccgctt ctttcaggac
      541 ttgatcccga tcccattttg ccaactttcg agcttcctcg gaagggcacg acgattttgg
      601 cttactgcag cggcaatttg aatcgattcc aggtacagca aacgatggcc ggcaagctgg
      661 ataataacct ggacatgcag gaaaaaattc atcaatccat ccagttcact tcgaaaatac
      721 agtacctgtg ggataagaaa aaaggtgtat tcagaagcaa gaaagtcaag tagaatgaac
      781 tgaccatagc cgccttcgag aaaagactga cactaatgtc agtctttggt ggcactgcgt
      841 tcgacttcgg gtaattttgt cctatgaaag acctcagaaa caaagtcgtg ctgaggcggg
      901 cggtatcggc catgtagaag tgacatgcgg caatataaaa tctaaccctt tagggcttaa
      961 gcgatccaca gctggtcatt ttgtctcctg aaaaacggtc tttacacctg agaaagacag
     1021 catctattat ggttggtagc aatttcttat ctgcttagac aaacatgagt attaactatg
     1081 aaaagtattt tcagaggcga agaattttgc ttaaaatcgc atcttttact ttcatttgcg
     1141 cttgcatctt atctgttagt gccgtttagg catccgaaga taccgcttgg acaaatccga
     1201 tttccagcca ggccattacc ttacttcagg gctgggaaaa cacagagaaa cctgagctcc
     1261 gcgacattgt ggcgactttt tcactgaggc aaatccatta aacttgcgct ctcgcaaaaa
     1321 acagacgcag acctgtcgtt gcgcgaattt gtaaggctct ggcatgtcgc cgctgagcgt
     1381 tccacaggta agaaaatcgc aaaacctcgg gtcatgaagg tgaagagtag ggaatttcat
     1441 gcgatggaga atgccaccag aacaggtgag gaagaaaagc ttgttgtaga accttattgg
     1501 attatgcact ttgacggaac ttcgcagttc agaaacaagg agcgctcggt tttgaaactc
     1561 taagtgccga cggttctacc ttattaggca tcaaaataca ctatttcaag aagatttggt
     1621 agcgagttgg cgcacaggct ctaaaggcgc aactatgtca gacaacgaag attcaatgac
     1681 acgataaaac gcgtctcttg acaacacctc gtcgtaacaa ctttaagccc atgctcgata
     1741 atgcaacggt cgaaaatctg atcgcccgtt atgattcccc cgtacctcgg tacacttcat
     1801 accccaccgc gcttgagttt aaaccgttgg aggatgctgc gcttaacgat agctatcaac
     1861 agtgcaaccc caaccatcct tttagcgtgt atatccactt gccgttttgt gagaaccagt
     1921 gtctctattg cggatgcaac gtactacttt cacggcgcaa agatattctg gacacgtata
     1981 ttccacttct tattcaagag atacaaagta aaacagagct tctcagtttt aggccaaaga
     2041 tcagtcaact tcattttggc ggaggcactc ctaatttttt gcgtttaccc gattgggata
     2101 aactttttga agcgcttcgt aaaaattttg attttaacga caatgctgaa atttcagtcg
     2161 aactcgaccc catgtatttg ggcgaggggt atctaaatca tttgcgcacg ttggggttca
     2221 atcgtgttag ctacggtgtg caagatgtga ctcccaaagt tttagaagcg gtgaatcgtc
     2281 cgcaagatat tgatcggatt cg
//
LOCUS       DAOMPD010000438         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18559_length_2295_cov_2.491964, whole genome shotgun sequence.
ACCESSION   DAOMPD010000438 DAOMPD010000000
VERSION     DAOMPD010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18559_length_2295_cov_2.491964"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLY93_12545"
     CDS             complement(<1..746)
                     /locus_tag="PLY93_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRP70354.1"
                     /translation="MTKLKFTTWKEVIEFAKKRGVLFYDFRFTDIVGAWHHVSYYVDA
                     IEEDSFKALPFDASSIAKWQPIHKSDMQLIPDLEGAFMDPFTADPTLVIFCDVYDIYK
                     GQMYEKCPRSIAKKSLEYLKSTGLGDVAYFGPENEFFIFDSIRVRDDINCQYYEIDSN
                     EGSWNTATANSNAGHNLGHRPGTKGGYFPVSPRDSQVEIRAEIVKTLNEVGIETFVVH
                     HEVAQAQGEVGIKFGTMVEAADNVQKLKYVV"
     gene            complement(766..1353)
                     /locus_tag="PLY93_12550"
     CDS             complement(766..1353)
                     /locus_tag="PLY93_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70355.1"
                     /translation="MRFLAIFFFIGIFLSATHTILAGTRAPLDKPRFKVLDEQVDLLL
                     LRNTPRYLVEGIGGAGMLLGETETKLRRFHGEPAYRDFASPETLYYSTEKFSGAFILK
                     HGRIYEIRLEIEKNKSPSLEWFTALGLHESDVRGKDTQDVLAFATKFYRTRRVRQVGD
                     KVDVFGRGIEFQLRGKSIIFVVIRAAEPYSDLRTD"
     gene            1337..2134
                     /locus_tag="PLY93_12555"
     CDS             1337..2134
                     /locus_tag="PLY93_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRP70356.1"
                     /translation="MAKKRNVSKAYQDPEFMTSVEARSLRILSEYLMPLTRFEKMRVK
                     DTIVFFGSARIPSPVEVKAERARLVAQGKTDDAAQRRFDARVRYYKDAVRLSYRLTNW
                     SKALPADARRFLICSGGGPGIMEAANRGAAYARGLSVGMNIALPFEQIPNPYITQNLN
                     FDFHYFFMRKFWLTNMAKALVAFPGGFGTFDELFEILTLVQTHKMAKRLPIVVYGKNY
                     WQDVVNFEKIADWGMISHEDLKYFHFADSPDEAFDFLTQHLTELYLK"
BASE COUNT          586 a          552 c          548 g          609 t
ORIGIN      
        1 accacgtact tgagcttttg tacgttgtcg gcggcttcga ccatggtacc gaatttaata
       61 cccacctcgc cttgcgcctg cgccacttcg tggtgcacca cgaaagtctc aattcccact
      121 tcgttgagtg tttttacaat ttcggcgcgg atttctacct gtgagtcgcg tggcgacacc
      181 ggaaagtaac ctccttttgt accagggcgg tgccccaagt tgtgaccagc gttagagttc
      241 gcggtagcgg tgttccacga gccttcgttg ctgtcgattt cataatattg acagttgatg
      301 tcgtcgcgca cgcggatgga atcaaaaata aagaattcat tttcagggcc aaagtatgcc
      361 acgtctccca aaccggtgga ttttaaatac tccaacgatt ttttggcgat tgagcgtgga
      421 catttttcgt acatttgccc tttgtagatg tcatacacat cgcaaaaaat aacgagagtg
      481 ggatcggcgg tgaaaggatc catgaatgcg ccttcgaggt cggggatgag ctgcatgtcc
      541 gatttatgga tcggctgcca tttcgcaatc gagctggcgt caaaggggag cgccttgaac
      601 gaatcttctt cgatggcatc gacgtagtat gaaacatggt gccacgcgcc cacaatatcg
      661 gtaaaacgaa aatcatagaa taaaacgccg cgcttctttg caaattcaat tacttctttc
      721 cacgtagtga attttagttt tgtcatggct ggcagtttta acgttttagt cagtgcgtaa
      781 atcgctgtac ggttcggcag cgcggataac tacaaaaatg atagatttcc ctcgaagttg
      841 aaattcaatc cccctaccga atacatccac cttgtctccc acctgccgca cgcgccgagt
      901 gcgataaaat tttgtcgcaa acgcaagcac gtcttgtgtg tctttgcccc gcacatcgct
      961 ctcgtgcaag cccaacgcag tgaaccattc taaagaaggc gatttatttt tttcaatctc
     1021 cagtcggatt tcataaatac ggccatgttt taatataaat gcgccgctaa atttttctgt
     1081 gctataatag agcgtttccg gcgacgcaaa atcacggtac gccggttcgc catgaaaacg
     1141 ccgcagcttg gtttcagttt cacccaagag catacccgca ccgccgatgc cttcgacgag
     1201 atagcgtggc gtgttccgca ataaaagtaa atccacctgt tcgtccaaaa ccttgaaacg
     1261 gggtttatcc aacggcgcac gcgtgcctgc aagaatcgtg tgcgtagccg ataaaaaaat
     1321 gccgataaaa aagaaaatgg ctaagaaacg caacgtgagt aaagcatacc aagaccccga
     1381 gtttatgacg tcggtagaag cccgctcgct ccgcatttta tcggagtatc tcatgccgct
     1441 cactcgtttt gaaaaaatgc gcgtgaaaga caccatcgtt ttttttggtt cggcgcgcat
     1501 cccttcgccg gttgaggtca aggccgaacg cgcacgttta gtggcacaag gcaagacaga
     1561 cgacgctgcg caaaggcgtt ttgacgcgcg ggtgcgttac tataaagacg cggtccgctt
     1621 atcgtatcgg cttactaatt ggtcgaaggc gttgcctgcc gatgcacggc gatttctcat
     1681 ttgctcgggc ggggggcccg gcatcatgga agcggccaac cgaggcgctg cgtatgcgcg
     1741 tggactttct gtgggcatga atattgcgtt accatttgaa caaataccca atccgtatat
     1801 cacccaaaac ctcaattttg attttcacta tttttttatg cgcaagtttt ggctcaccaa
     1861 tatggcaaaa gccctggtgg cttttccggg tggttttggc acgttcgatg aactctttga
     1921 aattttgacg cttgtgcaaa cgcataagat ggcgaaacga ttgcccatcg ttgtgtacgg
     1981 caaaaactat tggcaagacg ttgttaattt tgaaaaaatt gccgattggg gtatgatttc
     2041 gcacgaagac ttaaaatact ttcacttcgc ggattccccc gacgaagcgt ttgatttttt
     2101 aacacaacat ttaaccgaac tctatctaaa ataaatacct gatgaaatga aaaagtgtca
     2161 gacctattaa gtattcagag ccgaacagcc aagaaatctt gacgacgtac tatgtttctt
     2221 tcgcagaagc gaaaactagc tgacagcgca ctgcggcgcg ggtgggttgt attatgcgag
     2281 tatcggttat tgaag
//
LOCUS       DAOMPD010000439         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18610_length_2289_cov_3.041629, whole genome shotgun sequence.
ACCESSION   DAOMPD010000439 DAOMPD010000000
VERSION     DAOMPD010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18610_length_2289_cov_3.041629"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLY93_12560"
     CDS             <1..1341
                     /locus_tag="PLY93_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRP70357.1"
                     /translation="KNVARKATILADGLATKFGRLALGKNITVAFMPWDGYNFEDAII
                     LSEKLVREDTFTSIHIEEFEIQARETKLGREMITRDIPNISERAFRDLDDEGIIRVGA
                     EVRAGDILVGMVTPKGETDLTPEYRLLHSIFGEKAKEVRDSSLRVPNGHAGIVVDIVR
                     FKRDEGDELPAGIVEAVKVYVAQKRKISVGDKMAGRHGNKGVISTILNEADMPMLPDG
                     TPIDIVLNPLGVPSRMNLGQIFETQLAYIGKKLGIHFETPVFDGAEWHHIQEYAKKAN
                     VSHDSKILLRDGRTGDYFRQAAFVGTIYMLKLHHMVEDKMHARSTGPYSLVTQQPLGG
                     KAQFGGQRLGEMEVWALEAYGAAHTLQELLTVKSDDMQGRARVYESIVKGIQAIKPGV
                     PESFNVLIRRIRSLALDITILDHEEKPVEISDLDDEFSRVRRRIKVDSVENSAG"
     gene            1378..>2289
                     /locus_tag="PLY93_12565"
     CDS             1378..>2289
                     /locus_tag="PLY93_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRP70358.1"
                     /translation="MREFHDFHKIRIKLAAPDKIREWSRGEVKKPETINYRTLKPERD
                     GLFCEKIFGTTKDWECYCGKFKSIRYKGVVCDKCGVEVTHSKVRRERMGHIELSYPVA
                     HIWYYRTVPSRIALVLDMSANDIKSVLYFERYVVIEPSESGLEFKQLITQEEYSRLVE
                     LYGDKFYASIGADAVKELLAIVNIEEEIRLIRAKIESKKKISDKRILKRLEILEAFRD
                     SHNRPEWMVLDTIPVLPPELRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLGLKA
                     PDIIVSNEKRMLQEAVDALFDNSRRKKS"
BASE COUNT          614 a          528 c          601 g          546 t
ORIGIN      
        1 aaaaacgtcg cgcgtaaagc gacgatttta gcagacggtc ttgcgaccaa gtttggtcgt
       61 ttggcgttgg gtaaaaatat caccgtggct ttcatgccat gggacggtta taacttcgag
      121 gacgcgatta tcttgtcgga aaagttggta cgcgaagata cgtttaccag catccacatc
      181 gaagagttcg agatccaagc gcgcgaaacc aagctcggtc gtgagatgat cacccgagat
      241 attcctaaca tcagcgagcg ggcattccgc gatttagacg atgaaggtat catccgtgtg
      301 ggcgcagagg tgcgtgcggg agatattttg gttggtatgg tgacccccaa aggcgaaacc
      361 gacttaaccc ccgagtaccg tttactccat tctatttttg gcgaaaaggc caaagaagtg
      421 cgtgactctt ctttaagagt acccaacggt cacgcgggga ttgtcgtcga catcgtgcgt
      481 tttaagcgcg acgagggcga tgaacttccc gctggtatcg ttgaagccgt caaagtgtac
      541 gtcgctcaaa aacgtaaaat ttctgtcggg gataaaatgg ccgggcgcca cggaaacaaa
      601 ggcgtcattt ccacaatcct caatgaagcg gatatgccga tgctccccga cggcacaccg
      661 attgatattg ttttgaatcc gttgggggtt ccttcgcgga tgaacttggg gcaaattttt
      721 gaaacccagt tggcgtacat cggtaaaaag ttgggcatcc attttgaaac gccggttttc
      781 gacggagcag aatggcacca cattcaagag tatgccaaga aagccaatgt cagccacgac
      841 tcaaaaattt tattgcgcga cggccgtacg ggggattact tccgtcaagc ggcgtttgtc
      901 ggtaccattt atatgctgaa gctacaccac atggtcgaag acaaaatgca cgcgcgctcc
      961 accggcccct actcgcttgt gacacaacaa ccgttgggtg gtaaagcgca gtttggtggc
     1021 cagcgtttgg gtgagatgga agtgtgggcg ctcgaagcat acggtgcggc gcatacgcta
     1081 caagaacttt taacggttaa gtccgacgat atgcaagggc gtgcgcgcgt ttatgaatcg
     1141 atagtgaaag gtattcaggc gattaaaccc ggagtacccg agagcttcaa cgtgctcatc
     1201 cgtcgaattc gctcgttggc gttggatatt accatcctcg accacgaaga aaaaccagtt
     1261 gagatttccg atctagacga tgagttttca cgcgtacgcc gccggattaa agtagactcg
     1321 gttgaaaatt cagcagggta atgacaggta gataacgcaa aaggatagag gctaagaatg
     1381 agagaattcc acgattttca caaaattcgt atcaagctcg ctgctcccga taaaattcgt
     1441 gagtggtcgc ggggtgaagt aaagaagccc gagacgatta actaccgtac gcttaaacca
     1501 gagcgtgatg gtttgttctg cgagaaaatt tttggtacaa ccaaagattg ggaatgttac
     1561 tgcggcaaat tcaagtcgat tcgttataaa ggcgttgttt gcgataagtg cggcgtcgaa
     1621 gttacgcact ccaaagtccg ccgtgagcgt atgggtcata tcgagctctc gtaccctgta
     1681 gcgcatatct ggtactaccg cactgtgccc agccgcatcg cgttggtgct cgatatgtcg
     1741 gcaaacgaca ttaaaagcgt tctttatttt gaacgttatg tggtcatcga accgagcgaa
     1801 tcgggtcttg agttcaagca actcatcacg caagaagaat actcgcgctt ggtcgaactt
     1861 tacggcgata aattctatgc gtcgattggc gccgatgcag tcaaagaact tttagcgatt
     1921 gtaaacatcg aagaggaaat ccgcctcatc cgtgcaaaga ttgaatctaa gaaaaaaatt
     1981 agcgacaagc gtattttaaa gcgcttagaa attttagaag cgttcagaga cagtcacaac
     2041 cgtcctgaat ggatggtgct ggacaccatt cccgtgcttc ctccagagtt gcgtccaatg
     2101 gtgcaactcg aaggtggccg gtttgcaacg agcgacttga acgaccttta ccgtcgtgtc
     2161 atcaaccgca acaaccgctt gaaacgcctt ttaggtctta aagctcccga catcattgtt
     2221 tcgaacgaaa agcggatgct ccaagaagcg gtcgatgcac tttttgataa cagccgccgc
     2281 aagaaatcg
//
LOCUS       DAOMPD010000440         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18709_length_2279_cov_3.656475, whole genome shotgun sequence.
ACCESSION   DAOMPD010000440 DAOMPD010000000
VERSION     DAOMPD010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18709_length_2279_cov_3.656475"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="PLY93_12570"
     CDS             <1..1703
                     /locus_tag="PLY93_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRP70359.1"
                     /translation="TTSIAGAAGKSYYTFYGAYAAMRDTTVAQKRIFVLTFLASILAG
                     WLLITHPLSPSLKKRGENLCSPFSFQEKGAGGMSFGQRATAVVILVSFVTVNCGSGFA
                     PGSVPGDLADNKESIISALYTGLPAGTVYYSHNHLGSGALVTDANGEEVFRITYTEYG
                     EIDLHNSGKYNPETGAIEHHIDAALIAITAVKYTGQEYDPETGFYYYNARYYDPQLGL
                     FTTPDKKFDQNAGSFGFNRHMYVAGNPVMYNDPSGHFAFLAVLGAVLIKAAVGAAIGG
                     TVAATVQAARHNWDFANNWNSIGKAFGMGAAIGAATAVGGIAGKWASSGISSEFGRIF
                     TEQVVSRTVAGGLNGGINALYGNKNFLKGFGGGLVSGAVGGVFSGVSAGIGNVGFGVP
                     SILIDGVGNVASNAASRSMGFSSKSDGAAFLTGILNGGLRVGIRTLSAKISGAEFQRS
                     KYNTVGVAHRDTHIYKDLEASGNNPYALEYRQLSEAKYTADGDLNWKYEGSSVSDALS
                     LSVNETSQFHDGFNALVYHGGAQWLMETPFNELSMIPSYAIVQHLDQGSFWNNYETYR
                     "
     gene            1687..>2279
                     /locus_tag="PLY93_12575"
     CDS             1687..>2279
                     /locus_tag="PLY93_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70360.1"
                     /translation="MKLIANAIFIVATIINISCAGGSVSFKKNRIYAKEDVCNPWLGK
                     HDRYLKYKDYSVFSVNGEDLDTILRIDWDEWNMWYIILVVAPIPTYGFWKETSPAILN
                     FEIKNQRKNNIVLNPCEILFFSSDAGKVEKKNLKSIPNCSSLIPIIIKPKDKKFFTVN
                     YIAEEMNQLKQINLIFSEPAAKERIIQIIEIDDDWEY"
BASE COUNT          612 a          427 c          554 g          686 t
ORIGIN      
        1 cgacgacgag cattgcgggt gcagcaggca agagttatta cacgttctat ggcgcttatg
       61 ccgccatgcg cgatacgacg gtggcgcaaa agcgtatttt cgtgctcact tttcttgcct
      121 ccattttggc tgggtggctt ttgataactc accccctatc cccctctctt aaaaagagag
      181 gggaaaatct atgctctccc ttctcttttc aagagaaggg ggctgggggg atgagtttcg
      241 ggcaacgcgc caccgccgtt gtcatacttg tctctttcgt caccgtcaac tgtggtagcg
      301 gatttgcccc gggctctgtg ccgggcgatt tggcggataa taaagaaagt attatttcgg
      361 cactgtacac gggattgcct gctggtacgg tttattatag tcataatcat ctcggttccg
      421 gggcgttggt tacggacgcc aatggcgaag aagtttttcg gattacgtac acggagtatg
      481 gtgagattga tctgcataat tccggaaaat acaatcccga aacaggcgcc atcgaacacc
      541 atatagacgc tgcgctcatt gcgattactg cagtcaaata caccggccaa gaatatgacc
      601 cggaaaccgg tttctattat tacaacgcac ggtattacga cccgcagttg gggttgttta
      661 cgacgccgga taaaaagttt gatcaaaatg ctggtagttt tgggtttaat cgacatatgt
      721 atgtggccgg taatccggtt atgtataatg acccgagtgg gcattttgct ttcttggctg
      781 ttttaggtgc cgttctcatc aaagcagcgg ttggtgctgc tattggcggt acagtcgcag
      841 caacagtgca agctgcacgg cacaattggg attttgcaaa taattggaat tctattggca
      901 aggctttcgg tatgggtgcg gcaatcggtg ctgcgactgc cgttggcggc atagccggaa
      961 aatgggcttc atcaggtatt tcaagtgagt ttggccgaat ctttaccgag caagtggtaa
     1021 gccgaactgt tgcaggggga ttaaatggcg gtatcaacgc cctttacggt aataaaaatt
     1081 ttctaaaagg ctttggtggc gggcttgtta gcggagcagt cggtggcgta tttagtggag
     1141 tttctgcagg tattgggaat gttggctttg gcgtaccgag tatcttaatt gatggtgttg
     1201 gtaatgtggc ctcgaatgca gcttcacgca gtatgggttt ttcaagcaaa tcagacggag
     1261 cggcgtttct taccggaatt cttaacggcg gtcttcgtgt tggtattcga accctttctg
     1321 caaaaatctc tggtgccgaa tttcaaaggt cgaagtataa taccgttggg gtcgcgcata
     1381 gagacacaca catttataaa gacttggaag caagtggcaa taatccttac gcacttgaat
     1441 atagacaact ttcagaggca aaatatactg cagatggtga tttaaattgg aaatatgaag
     1501 gctcttctgt ttctgacgca ttgagcttgt ctgtaaatga aacatcacaa ttccatgatg
     1561 gttttaatgc tcttgtttat catggtgggg cacaatggct tatggaaacc ccattcaatg
     1621 aactgagcat gataccatct tatgcgatag ttcaacactt agatcaaggt tcattttgga
     1681 ataattatga aacttatcgc taatgcaatt tttatcgttg caacaataat taatatttct
     1741 tgcgcgggtg gttcggtttc ctttaagaaa aatcgaattt atgctaagga agacgtatgt
     1801 aatccttggc tgggtaagca tgatcgttat ttgaaatata aggattatag cgtcttttca
     1861 gttaatgggg aagatttaga tacaatattg cgtatcgatt gggatgagtg gaatatgtgg
     1921 tatattattc ttgtagttgc tcctattcca acctatggct tctggaaaga gacgagccct
     1981 gctattttga attttgaaat caaaaatcaa agaaaaaata atatcgtttt gaatccttgc
     2041 gagattttat tcttttcttc tgatgccggt aaagttgaaa agaaaaatct aaaatcaatt
     2101 ccgaattgta gctcacttat tcctatcatt attaagccaa aagataagaa attttttact
     2161 gttaattata ttgcagaaga aatgaatcag ctaaaacaga ttaacctcat tttttcagaa
     2221 cctgctgcga aagaaagaat tatacaaatt attgaaattg acgatgactg ggaatactg
//
LOCUS       DAOMPD010000441         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18737_length_2276_cov_2.731652, whole genome shotgun sequence.
ACCESSION   DAOMPD010000441 DAOMPD010000000
VERSION     DAOMPD010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18737_length_2276_cov_2.731652"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PLY93_12580"
     CDS             <1..1336
                     /locus_tag="PLY93_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRP70361.1"
                     /translation="RGMTGGFPGLFSMHPYYVKINEYNDMESRDLWEYRLNLTASELH
                     FLEAHLWELSYAYFPYYYLDENCSYQILALLEAVRPEVRLNNRFSYFVAPTDTLHTLS
                     ENNFFTGKSQYRASSATRYTTFYENATPAARETMVLLKENRTLPQTHTSDDTQAVDIL
                     LEYYKYTNEYNMALWPETDKEHYQTLLAWRAQVPHENALENSVRARAEDNPLVGFKSS
                     EIAVGYAAQDNLGNATTLRFRPALRELTDASRGFSPWSQMQMMQFSFAYFFEKQHLRL
                     EEANIVDIFALSPWQAHIRKISYRLRTGLYRHDALQNYNAATLSWDSTAGIGITLLPL
                     KSIGFFVLAEANAYVTPTWQSAARIAPSLYTGFKFNWSRAFASTLSYRIDYAFFSPAL
                     IEQKIETRNALAVTDSTALEVKFFYAWAAVETLPPTTRLDAMRGDASLKLYF"
     gene            complement(1697..>2276)
                     /locus_tag="PLY93_12585"
     CDS             complement(1697..>2276)
                     /locus_tag="PLY93_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70362.1"
                     /translation="ADEPREMRHTISITGMAPTMVLIYNYGAGYDYLLTKPIDTWGLT
                     ASASIKSGYLWLPLAGNNLLGAGRTLRSDLGVKAWWRVELENSRFDFFLGLSGVYIQE
                     KHILYYQDSSKLPSVEVGRQLFSRILPGFALGIVFRPQKISHWSFGMSFNGLLNNSAN
                     MTYLVPNGATLQNTGISLGRGLFWEFLVGYSF"
BASE COUNT          635 a          585 c          482 g          574 t
ORIGIN      
        1 gcgcggcatg acggggggat ttcccggttt attttcgatg cacccctact acgtaaaaat
       61 caacgaatac aacgacatgg aaagccgcga cctgtgggag taccgcctta acctcaccgc
      121 aagtgaactg cattttttag aagcgcattt gtgggaattg agttacgcgt attttcctta
      181 ttattattta gacgaaaatt gttcttacca aattttagcg ctgttggaag cggtgcgtcc
      241 agaggtacgc ctcaacaacc gctttagtta tttcgtcgcg cctaccgaca cgctgcacac
      301 gctttcagaa aataattttt ttacgggtaa aagtcaatac cgtgcatcga gcgcgacgcg
      361 ttacacgacg ttttatgaaa acgccacgcc ggcagcacgc gagacaatgg ttctccttaa
      421 agaaaatcga actctaccac aaacgcacac gagcgacgac acccaagcgg tggatattct
      481 tttggagtac tataaataca ccaacgaata caatatggca ctctggcctg aaacagataa
      541 agagcattac caaacactac ttgcatggcg cgcccaagta ccgcatgaaa atgcactcga
      601 aaattctgtg cgcgctcgcg ctgaagacaa tccccttgtg ggttttaagt cgtctgaaat
      661 tgccgtaggc tatgccgccc aagataattt gggcaatgct acgacgctgc gctttcgccc
      721 tgctctgcgc gaactcaccg acgcctcgcg cggattttca ccgtggtcgc agatgcaaat
      781 gatgcaattt tctttcgcgt acttttttga aaaacaacac ttgcgtttag aagaagccaa
      841 catcgtcgat atttttgcac tgtcgccgtg gcaggcgcat atacgtaaaa tatcgtaccg
      901 gttgcgcacc ggcctttacc gccacgacgc gctacaaaac tacaacgcag cgacactctc
      961 atgggattca accgccggca tcggcatcac gcttttgccg cttaaaagta taggcttctt
     1021 tgtgctcgcc gaggccaatg cgtacgtgac gcccacatgg cagagcgcgg cacgcatcgc
     1081 gccttcactc tacacgggct tcaaattcaa ctggtcgcgt gccttcgctt cgacattatc
     1141 gtaccgcatc gattacgcgt ttttctcgcc cgccctaatc gagcaaaaaa tagaaacgcg
     1201 taatgcgctg gccgtaacag acagcaccgc actcgaagtc aaatttttct acgcatgggc
     1261 ggcggtcgaa acgctgccac ccacgacgcg cctcgacgcc atgcgcgggg atgcatctct
     1321 taaattgtat ttttagttta gtgtcgcttg agtcaaaata ccaccaaact ttgccaccca
     1381 cgtccgaacg caaaaaatca cttttactca taagcttatc gtcgcccggc acaccggatg
     1441 aataaccata tcggcctgct gcccccactg aaaaccccag atttttattt gggttggtga
     1501 gcagatagtc gtagcttaaa ccaaaatgat atgtgaaaag aaaaatgggt attgcaatac
     1561 cggttactgc aagcgtgtgt ttttcttctt tgtcttgaga taagatttga tggggtaaaa
     1621 ataatacgag tagaatgaaa atgtattttt tatgcgattt catgttttag caccaaagcc
     1681 ccagagttag cacgcattag aaagaataac caaccaagaa ctcccaaaac aaaccacgcc
     1741 ccaatgaaat accggtattt tgtaacgttg cgccattggg gaccagataa gtcatatttg
     1801 cgctgttatt cagtaaaccg ttaaatgaca tcccaaaact ccagtgtgat attttttgcg
     1861 gacgaaaaac tatacccaaa gcaaatccag gtagtatgcg gctaaatagt tgtctgccaa
     1921 cctctacact tggcaatttg ctagaatctt gataatacaa tatatgcttt tcttgaatat
     1981 acaccccgct taaaccgaga aaaaaatcaa aacgactgtt ttcaagttca acacgccacc
     2041 atgctttaac acccaagtcg gagcgcaacg ttctacccgc acctaacaaa ttattgcccg
     2101 ccaatggaag ccacaaatag ccggatttta tagaagcact ggcggttaat ccccatgtgt
     2161 cgatgggttt agttagaaga taatcatagc ctgcaccgta gttataaatc aagaccattg
     2221 ttggcgccat tcccgtaatc gagattgtgt gcctcatttc ccgaggttcg tctgct
//
LOCUS       DAOMPD010000442         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18828_length_2266_cov_2.653098, whole genome shotgun sequence.
ACCESSION   DAOMPD010000442 DAOMPD010000000
VERSION     DAOMPD010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18828_length_2266_cov_2.653098"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /locus_tag="PLY93_12590"
     CDS             <1..2120
                     /locus_tag="PLY93_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70363.1"
                     /translation="ISTGLWFIADAIPSFFFDKKALQARLNEASDMSGLAISYQGIDV
                     SLSRGVRILGVRISFDKEFARGRYLLEAPAVTIRVPFSFPSTADANLLTRARLIIESG
                     KLNYFITNDDTDREMLNEVHRLLQRNLRYHIECNDCSFALHVKDNNYFQEITPVKTVF
                     FTVRHIGEEIEALLHYESSTIGDGDFFAKFKACATSACTDIEGYWYVKPVGLKTAIFN
                     NFQKKYHIQSGLLSGEMAFDRNIVDTEKTVRGKKITVREPVSNFRLSASAKNFVLSQK
                     NEEWYRADSFIVDTKILIHGPSSTGYVSATLEDYNIHVEFDDLRADALPERYTFRVEP
                     QRFSDKTLHLPTQKKITGLDKLEINLSERKGDKYQKAEAHVQIKSGNFYLADEKKLPP
                     IRIENTDFTLADEKFTGNLQATLGTSRVDFTSNGSLTLYPVSFKPRLNALIREHDEVE
                     ARQIFALKGRIAATLKSPSLLWDDLKPYVNFWLDDNWQEVREGMHYSWMPSHLKRREY
                     FVRFFQYLDFSMPIEIQSFNWGEQTPLRGELFFAPQYTGGGFRLSAGDGKNWTNLSVS
                     YGGDEASAPWYTHNLRLNINGGYELLKPWFGGEFFEYFSGVELAYDSNYNGERAADHY
                     LKVNSVTDLRLKRVRLGAWARKRQIPLQWETVDVRANRSNGFGTISSVRAENENTIIS
                     GYGDYKLFDRDLDVNLKHSILIK"
BASE COUNT          617 a          514 c          536 g          599 t
ORIGIN      
        1 ttataagcac tgggttgtgg tttatcgccg atgcgatccc cagcttcttt tttgataaga
       61 aggcgttgca agcgcgattg aacgaagcga gcgatatgag cggcttggcg atttcatacc
      121 aaggtatcga tgtcagtttg tcgcgcggtg tgcgcattct tggcgtgcgt atctcgttcg
      181 ataaagaatt tgcgcgtggg cgttatttac tcgaagcgcc ggcggttaca atccgcgttc
      241 ctttttcatt tccttcgacc gccgatgcaa acttgctcac gcgtgcccgc ttaattattg
      301 aatcgggaaa gctcaactat ttcatcacca acgacgacac cgaccgcgaa atgttgaacg
      361 aagtgcaccg tcttttacaa agaaatttgc gttaccacat tgaatgcaac gattgtagtt
      421 ttgctctcca cgttaaagac aataattatt ttcaagaaat tactccggta aaaactgttt
      481 tttttaccgt gcgtcacatt ggagaggaaa tcgaagcgtt gttgcattac gaaagttcga
      541 ccattggcga cggcgatttt tttgccaaat tcaaagcgtg tgcaacatcg gcttgtacag
      601 atatcgaagg gtattggtat gtaaaaccag tgggattaaa aacggcaatt tttaataact
      661 ttcaaaaaaa ataccacatc caatcggggt tactttcggg agagatggct ttcgaccgca
      721 acatcgtgga caccgagaaa acagtgcgag ggaaaaaaat aacagtgcgc gaacctgtct
      781 ccaattttag attgtcggca tcggcaaaaa attttgtgct ctctcaaaaa aatgaagagt
      841 ggtaccgcgc ggattccttt atcgtagaca caaaaatctt aatccacggc ccaagctcaa
      901 ctggctatgt gagcgcaact cttgaggact acaacataca cgttgaattt gacgacttac
      961 gtgccgatgc tctgcccgag cgctatacat tccgcgtcga accgcaacgt tttagcgaca
     1021 agacgctaca cctgccaaca cagaaaaaaa ttacagggct ggataagtta gagatcaatc
     1081 tttccgaacg caaaggcgat aaataccaaa aagccgaagc gcatgttcag attaaaagcg
     1141 gcaacttcta tcttgccgac gaaaaaaagt taccgccaat tcgaatcgaa aatacggact
     1201 tcacgcttgc cgacgagaaa tttaccggca accttcaagc gactttgggt acatcgcgtg
     1261 tcgatttcac ctcgaatggt tcgctgacgc tttacccggt gtcgttcaag ccccgtctca
     1321 atgctctcat ccgcgaacac gacgaagtag aggcgcgcca aatttttgcg ctcaaaggcc
     1381 gcatcgccgc aacattgaaa tccccttctc ttttgtggga tgatttaaag ccttacgtta
     1441 atttttggct cgacgataat tggcaagagg tgcgcgaagg gatgcactat tcatggatgc
     1501 catcgcactt gaaacgccgc gagtactttg tgcgcttttt tcagtattta gatttttcga
     1561 tgccgattga aattcagagt tttaattggg gcgagcaaac accgctgagg ggcgagcttt
     1621 tttttgcccc ccaatacaca ggcggtggct ttcggttaag cgcaggcgat ggcaaaaact
     1681 ggacaaacct ttctgtgtcg tatggcggcg acgaagcgag tgcgccttgg tacacacata
     1741 atttacgttt gaatataaac ggtggctacg aacttttgaa gccgtggttt ggcggtgaat
     1801 tttttgaata cttctcgggg gttgaattgg cgtacgacag taactacaac ggcgaacgcg
     1861 ctgccgacca ctatcttaaa gtaaactcag tcaccgattt acggttgaag cgtgtgcgtt
     1921 taggcgcgtg ggcgcggaag cggcaaattc ccttgcaatg ggagactgtc gatgtacgag
     1981 caaatcgttc caatggtttt gggaccattt catcggtgcg cgctgaaaat gaaaacacca
     2041 tcatatctgg ttacggggat tacaaactct ttgatcgtga cttagacgtg aatttgaagc
     2101 attcgatttt aattaaataa gaagataaaa gttttgcaat agccaaaccc tcctcgaaag
     2161 caattaatat gccttaaaaa tatacgccgc accagcctga cttacagagg tgtcgctgga
     2221 ggcggcgcca tccgtattcg ttaccgtcgt ttgattagag ctttct
//
LOCUS       DAOMPD010000443         2257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18922_length_2257_cov_2.348774, whole genome shotgun sequence.
ACCESSION   DAOMPD010000443 DAOMPD010000000
VERSION     DAOMPD010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18922_length_2257_cov_2.348774"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..782)
                     /locus_tag="PLY93_12595"
     CDS             complement(63..782)
                     /locus_tag="PLY93_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70364.1"
                     /translation="MTRYITILSFLIFPSWIMGVVPMLDMVNTPTAQTLKRGEFNVAL
                     TAYEGGGIFNRNMIGIHDNIYLGAAFDVENAIGYNTAKMNIPGVIAKAKLTDGWADFP
                     ILLAVGYDAFYASRYGKVNTGNPYNRMIFGPYFSITKPIYLLGDEQHIHFGTRMPVYP
                     NYTPEDTELYIGIDFPMGMFIPIFEIQRILFDANRFKEILFNLGFRFQFFDHLAFEID
                     FMMGIDQRTNRMLVFEYMDRF"
     gene            912..>1221
                     /locus_tag="PLY93_12600"
     CDS             912..>1221
                     /locus_tag="PLY93_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70365.1"
                     /translation="MARITDISDSSTENKNYNFDEPTMKRERNKTMTLIFSAGVVLMI
                     AVVAAVVYYWLWQKKQTLVETRLQPATQFEEANSRKMAPDQLPQDLELKQAVDMYNRG
                     Y"
     assembly_gap    1222..1252
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1303..>2257
                     /locus_tag="PLY93_12605"
     CDS             1303..>2257
                     /locus_tag="PLY93_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP70366.1"
                     /translation="MALVYLGIMADDEGKFNLAEDFLNRAIKFDSTNFYAHYNRAIAL
                     RHKGMYQEALAALERAQRLRPDLVEAQNLKGQLQYQNQDLKGAEETLKHAIETSKDPL
                     AYYNLGMVYKKDGKFAEAKSAFLDALNVASAGEIAYKAATQLGMLHATQGDLPNAKYY
                     FERAVALSPATAKYHYNLALVLHQLGESERAVRELDEAIKLGSENPQTYMYVARLYSE
                     LGRPQDAEKALERALQEAPKDPVILSQLGDMLIQQSKWAEAIQIFKQLHESSPKTKDK
                     ADALYNLGKIYVSVRDFKNAEKALEGAYELDMTNEDALVLLA"
BASE COUNT          663 a          469 c          547 g          547 t
ORIGIN      
        1 cccaatcacc ccgtggccga aggacaaact cgtgatcttc atggaatcct aaccaaccac
       61 tttcaaaatc gatccatata ctcaaacacg agcatgcggt tggtgcgctg gtctatcccc
      121 atcatgaaat caatttcaaa cgcgagatgg tcgaagaatt gaaatctaaa tcctaagtta
      181 aaaagaattt ccttgaaacg gtttgcatca aagagaatgc gttggatttc aaaaataggt
      241 ataaacatcc ccatcggaaa atcgatccct atataaagtt cggtgtcttc gggggtgtaa
      301 ttgggatata cgggcatgcg cgtgccgaag tggatgtgct gctcgtcgcc taaaagataa
      361 atgggttttg tgatgctaaa atagggaccg aatatcatac ggttgtaagg atttcccgta
      421 ttgacttttc cgtaacggct tgcataaaag gcatcataac ccacagccag caaaatgggg
      481 aagtccgccc aaccgtcggt gagttttgct ttagcaatca ctccggggat attcatctta
      541 gcggtgttgt aaccgatggc attttcaacg tcgaacgccg cgcctaaata aatgttatcg
      601 tggatgccaa tcatgttgcg gttaaagata ccaccgcctt cgtatgcagt gagcgcgacg
      661 ttgaactcgc cgcgcttcaa tgtttgtgca gtgggcgtgt tgaccatatc gagcatggga
      721 acaacgccca taatccacga gggaaagatt agaaaagaaa gaattgttat atatcgtgtc
      781 atattaacga gtttagtatt ttttgtggta tttaattggc gctgatagca accaatccaa
      841 tgcccatcct ttatatagtt atcggttaaa aatcacacta tggaaaagta aaatattacc
      901 gaaaatgaaa tatggcgcgt atcaccgaca tcagcgattc ttcgacagaa aataaaaatt
      961 ataattttga tgaacccaca atgaagcgag agcggaataa gacgatgacg ctcattttct
     1021 ctgcgggtgt tgttttgatg attgccgtgg ttgcagcggt ggtttattat tggttatggc
     1081 aaaaaaaaca aactcttgtg gaaacgcgtt tacaaccggc gacacaattt gaagaagcca
     1141 atagccgcaa aatggcgcca gaccaattgc cccaggattt ggaactcaag caagcggtgg
     1201 atatgtataa tcgtggctat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgcgttt
     1261 cagcagttga ttgaatcagg caaaagtgat gcggtaaaaa gtatggctct tgtttacttg
     1321 ggtatcatgg ccgacgacga aggtaaattt aatcttgcgg aggatttttt aaatcgtgca
     1381 attaaattcg attccacaaa tttttatgca cactacaata gagccattgc gttgcggcat
     1441 aaagggatgt accaagaagc gttggcggcg ctcgaaaggg cgcaacggct acgccccgat
     1501 ctcgttgaag cgcaaaatct caaagggcag ttgcaatatc aaaatcaaga tttgaagggc
     1561 gccgaagaaa cattgaagca cgcgattgaa acctcgaaag atccgctcgc gtattacaac
     1621 ttgggaatgg tatataagaa agatggaaaa tttgccgaag ccaagtcagc atttttagat
     1681 gcattgaacg tcgcctctgc cggcgagatt gcgtacaagg cagcgacgca gttggggatg
     1741 ttgcatgcca cacagggaga tttgcccaac gccaagtatt atttcgagcg tgctgttgca
     1801 ctatcgcctg cgacggcaaa gtaccattac aacttagcgt tggtattaca ccaattaggg
     1861 gaatcggagc gcgcggtgcg cgagctcgat gaagcgatta aactcggtag cgagaatccg
     1921 caaacgtata tgtacgtagc gcggctttat tctgagttag gccgtccgca agacgcagag
     1981 aaggcactgg agcgggcgct ccaagaagcc cccaaagatc cggtaatcct ttcacagctg
     2041 ggcgatatgc tgattcagca atcgaagtgg gcagaagcga tacaaatttt caaacagctt
     2101 catgaaagct ctcccaagac caaagataag gcagatgcac tctataactt ggggaaaatt
     2161 tatgtgtcgg tacgggattt caaaaatgca gaaaaagcac tcgagggtgc gtacgagctc
     2221 gacatgacga acgaagacgc tctggtgctt ttggccg
//
LOCUS       DAOMPD010000444         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18956_length_2252_cov_3.459263, whole genome shotgun sequence.
ACCESSION   DAOMPD010000444 DAOMPD010000000
VERSION     DAOMPD010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18956_length_2252_cov_3.459263"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..922
                     /locus_tag="PLY93_12610"
     CDS             131..922
                     /locus_tag="PLY93_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP70367.1"
                     /translation="MADKCPKCVQKVPEYMATYGDFITLILCFFIAIYQPPAPIKVSE
                     MRIMLSPFQGARGIMPGGNTLSKQKLEDMGLTVESMPAEAKGKVQSKDKFQIKQIFED
                     EIKKKKIKITEDERGIVISLIGDGYFAPGSAKLTEEGRRILVKISDVLARMDSYTRIE
                     GYADDEMTARPAGEFYESAWELAAQRAINAVRFLEEKNEVPPEKLSAATYGKTRQLTQ
                     SGTPEARALNRRIDVIILSGKNQSRTYKDNALPEGRVPQTETSVP"
     gene            complement(970..>2252)
                     /locus_tag="PLY93_12615"
     CDS             complement(970..>2252)
                     /locus_tag="PLY93_12615"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRP70368.1"
                     /translation="LFFMFTFTAHYSYILYVLLVFIGVLVGLEIPILLRILKERMSFR
                     DLVARVLSLDYVGGLVAAISFPLLLLRPEVGLVRASLLAGVVNVVVAFFAIYIFQKQI
                     RLRAQLLKASAVLLLLIGALVYSKPLQGFLDDELHADPVVYSKQSNYQKIVMTSWHEH
                     FSLFLNNNLQFNSFDEYRYHESLVHAPVQMYLDHQMQANTKNKKINVLVMGGGDGLAI
                     RELLRYENIEHIDLVDIDPAMTDLAKNHIWLKRLNHNAFSDTRVTIHNEDAFIYLQKP
                     KKLYDVVILDFPDPGNFSIGKLYSDIFFYRLKNVLADGAVGVTQSTSPFVARASFWCI
                     YNTIASTGYHALPYHVYVPSFGEWGFVAFSLKPIAMPRKLYLEKSMRFLNKDILATMS
                     NFPSDMAHVATHTQTLMSQSLVHYYEKEWQRTTR"
BASE COUNT          711 a          510 c          477 g          554 t
ORIGIN      
        1 gaaggtgtga tgtccatcca ggccggcgag aaccccaaag ttattatgga caaacttctt
       61 tgcttcgtag acccctcttc gcgtgaaatg ctcattaagc aacgcgaagc cgaaggcggc
      121 ggcaactaac atggccgaca agtgtcccaa atgcgtacaa aaagtcccag aatacatggc
      181 gacttatggg gacttcatta cgctcatctt atgttttttt attgcgattt atcaaccccc
      241 tgctcccatc aaagtctcag aaatgcgtat tatgctttca ccctttcaag gagcgcgcgg
      301 tatcatgccg ggcggtaaca ctctctcaaa acaaaaatta gaagatatgg gcctcactgt
      361 cgagtcgatg cctgccgaag caaaaggaaa agttcaatcg aaggataaat tccaaatcaa
      421 gcaaattttt gaagacgaaa taaaaaagaa aaaaataaaa attaccgaag acgaacgcgg
      481 tattgttatt agtcttatcg gtgatggcta ttttgcgcca ggttcggcca aacttacaga
      541 agaaggtcgc cgtattttag taaaaatatc cgatgtactt gcgcggatgg attcgtacac
      601 acgcatcgag ggctatgctg acgatgaaat gactgcgcgc cctgctggcg agttttacga
      661 atcggcgtgg gaactcgcag cacagcgagc catcaatgca gtgcgatttt tagaagagaa
      721 gaatgaggta cctcctgaaa aattatcggc ggcgacttac ggtaaaacgc gacaacttac
      781 tcaatcggga acacccgaag cgcgtgcact caacagacgc atcgacgtga tcatcttatc
      841 gggtaaaaac caatcgcgta cgtataaaga caacgcactc cccgaggggc gcgtgcctca
      901 aaccgaaaca agcgtacctt aacaaggatg gttaatgggc tgccgaacag ccattaccgc
      961 ccttaaatac tatcgcgtcg tgcgttgcca ttctttctcg tagtaatgca cgagactttg
     1021 cgacatgagt gtttgggtat gcgttgcaac atgcgccata tcactgggaa aattagacat
     1081 cgttgcaaga atatctttat ttagaaaacg catactcttc tcaagatata actttcgtgg
     1141 catcgctatc ggtttcaatg aaaacgccac aaagccccat tctccaaaac ttggtacata
     1201 aacatgataa ggcaacgcgt gataccctgt cgatgcgata gtattgtaga tacaccaaaa
     1261 cgatgcccgt gcgacaaaag gcgatgtcga ttgagtaact cccactgctc cgtcagctaa
     1321 aacattcttg aggcgataga aaaaaatatc tgaatataat ttaccgatag aaaaattacc
     1381 tggatctgga aaatctaaaa taactacgtc gtagagcttt ttgggtttct gtaaataaat
     1441 aaaagcgtct tcgttatgta tcgtgacacg cgtatctgaa aatgcgttgt ggttaagtct
     1501 ttttagccaa atatgatttt tagctaaatc tgtcattgct ggatcaatat cgactaaatc
     1561 gatatgctca atattttcat accgcaataa ttctcgaata gcaagtccat ccccaccgcc
     1621 cattacaagg acattgattt ttttattttt tgtgttcgct tgcatttgat gatctaaata
     1681 catttgcacc ggtgcatgca ccaatgactc gtggtagcgg tattcgtcga aactattaaa
     1741 ctgcaaattg ttattcaaaa ataatgaaaa atgttcatgc cacgaagtca taacaatttt
     1801 ttgatagttc gattgcttcg agtacacgac gggatcggcg tgtagctcat cgtctaaaaa
     1861 tccttgcagc ggttttgaat aaaccaaagc tccaatgagt aacagaagta ccgcactggc
     1921 tttcaatagt tgagcacgca aacgaatttg cttttgaaat atatagattg caaaaaacgc
     1981 cactaccaca tttacaacgc ccgccaaaag cgatgcgcgc acgagcccca cttcggggcg
     2041 caaaagcaac aacgggaaac taatcgccgc gacaagcccg cccacataat ccagcgagag
     2101 cacccgtgca accaaatcgc gaaacgacat gcgctctttg agaatgcgca ggagaatcgg
     2161 aatttcaagc cccactaaaa cgccgataaa aacgaggagt acataaagaa tatacgaata
     2221 gtgtgcggta aacgtgaaca taaaaaatag aa
//
LOCUS       DAOMPD010000445         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18967_length_2251_cov_2.960838, whole genome shotgun sequence.
ACCESSION   DAOMPD010000445 DAOMPD010000000
VERSION     DAOMPD010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18967_length_2251_cov_2.960838"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLY93_12620"
     CDS             complement(<1..297)
                     /locus_tag="PLY93_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70369.1"
                     /translation="MSLVSTFLESQSDVGSACAGEITQTQQVSTIKESTLRALFLLFG
                     IAFGYILIRAGVSSYDVIRDMFLFKSWHMYGVLGVAVPVSFIGLQLFRRTRRKSL"
     gene            complement(297..794)
                     /locus_tag="PLY93_12625"
     CDS             complement(297..794)
                     /locus_tag="PLY93_12625"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRP70370.1"
                     /translation="MSPEYIWPFWLGGLMIAFISIALVVTTGKFLAVTRGYVSACAVF
                     SRLKYFQKRNVGGLFSYSNLFTVGLIVGGFIATINTVGYHPTWDLGEFNAIWGDSLLV
                     KGVVLTLGGFLWGLGSRLAKGCTSGNAISGLSKGSVGSLAATCGFLIAGVVVTFAISI
                     ISGVK"
     gene            complement(1426..>2251)
                     /locus_tag="PLY93_12630"
     CDS             complement(1426..>2251)
                     /locus_tag="PLY93_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70371.1"
                     /translation="APRIFSLLVLILGVSPLLGPTIGGFIALHFSWRVLFLTLVLIAL
                     FLMLLVRFFLIGAKAADKEVSIRVKAMVVTYKAILKFKGFHTYAFAGAFSFSTLFVYV
                     AASPIIFMQHYTLSPQAYSLIFALIAGGFLLASQLNVVLGKRIRPEKVFRVTLLIQLI
                     SSALFLSAALVGFLNVYSTFFFLFVCLSCIGMSNPNASALALAPFSRHVGSASALLGF
                     VQTGISALVSVAVAPLGPYGLSAIVSLFFVTTLIAVVILFWAGRRSPNSHSMVLVG"
BASE COUNT          754 a          531 c          482 g          484 t
ORIGIN      
        1 gagcgacttg cggcgggtgc gtctgaaaag ttggagaccg atgaacgata ctggaaccgc
       61 tacgcccaaa acaccgtaca tgtgccacga cttaaaaagg aacatatcgc gtatcacatc
      121 gtagctcgac acccctgcgc ggattaaaat atacccaaac gcaataccga aaagaagaaa
      181 aagcgcgcgc aatgtacttt ctttaatagt acttacttgc tgtgtttgcg taatctctcc
      241 cgcacacgca gaacctacat cgctttgact ttctaaaaaa gtactcacta aactcattat
      301 tttactcctg aaataataga aatcgcaaac gtgacaacaa caccggcaat gagaaatcca
      361 caagtagcgg caagactacc cacagagccc ttcgagagtc ccgaaatggc gttacccgac
      421 gtgcaacctt tagcaaggcg tgaccccaat ccccaaagaa agccacctaa agtcaggaca
      481 acccctttca caaggaggct gtcaccccag atagcgttga actcgcctaa atcccaagtg
      541 gggtgataac ccaccgtgtt gatagtggcg ataaaaccac ccacaatcaa ccccacggtg
      601 aaaagattac tgtatgaaaa aagtccgccc acattccgct tttgaaaata cttgagtcgt
      661 gaaaacacag cgcacgcact cacgtaaccg cgtgtgacgg ctaggaactt tcccgttgtg
      721 acgacgagcg caatcgaaat aaaagcgatc atcagaccgc ccaaccaaaa tggccatata
      781 tattcaggtg acatagttat cctcctgaga acagcttaac aaatgttcaa tataacgtcc
      841 agaattattc gttttattgg atggaagttt aatgtgtgaa ataaccggta aagcagagga
      901 taactatcca tgcgaataat ttaggtaaaa aatatgattt ttttcatgaa atttgtactt
      961 tttttttcca aagtgagaaa atttgaccgt tttttggtat ttaatatata gagggcaata
     1021 tgttaaagac atccatcagt ttaccacaaa aaagtatcgc aacttggacg ataaaccact
     1081 gggcaattat gcggatgtac gcacggtttt tgcgaattgt cgagcgtaaa agagaatttc
     1141 agcgtggagt aacgcggcaa tacaactggg ggaatgggca ttgtgtgatt gttaaagtaa
     1201 aattcacgcc caaagaatac gattcactgc ataccgttgc agcgggtata cgggtgagtg
     1261 tatcatacct catttattac cttatccaat tctgggaaaa aatgtcgaaa aaaagcgcca
     1321 aattcgtcgt tgggggtaac tatacgccca aagtgctgga gtggtcaaaa aggagggtaa
     1381 attttgagga aaaacttcct tttaggcggg ccgggtaccg attcttcagc caactaagac
     1441 catactgtgg ctattcggac tcctgcgccc tgcccaaaaa agaatgacta ccgcaataag
     1501 cgttgtcaca aaaaaaagcg atacaattgc tgacaaacca tacggcccca acggcgccac
     1561 tgccaccgat acaagcgcag aaataccagt ttgcacaaat cccaaaagcg ccgatgcact
     1621 accgacgtgg cggctaaaag gcgccaacgc taacgcgctt gcgttggggt tactcatacc
     1681 gatacacgaa aggcaaacaa ataaaaagaa aaatgtcgag tacacgttca gaaaccccac
     1741 taaagccgcc gatagaaaga gcgcactact tatcagttgg attaaaagtg taactcgaaa
     1801 aactttttca gggcggatgc gtttgcccaa aacgacgttg agttgactcg ctaaaagaaa
     1861 accaccggca atgagcgcaa aaatcaaact atacgcttga ggcgaaagcg tatagtgctg
     1921 cataaaaata atcggcgaag cggcgacgta cacaaaaagc gtagaaaacg aaaacgcccc
     1981 tgcgaacgca tacgtgtgga accccttaaa tttaagaatg gctttatacg tcaccaccat
     2041 cgccttcaca cggatggaca cttctttgtc tgccgctttt gcaccgataa gaaaaaatct
     2101 taccagcagc atgagaaata gcgcgatcaa cacaagcgtc aagaaaagta cacgccaact
     2161 aaaatggaga gcgataaatc caccgatggt agggcctaag agtggcgaca cgcctaaaat
     2221 caacaccaag agcgaaaaaa tacgcggtgc g
//
LOCUS       DAOMPD010000446         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18973_length_2251_cov_2.035974, whole genome shotgun sequence.
ACCESSION   DAOMPD010000446 DAOMPD010000000
VERSION     DAOMPD010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18973_length_2251_cov_2.035974"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>2251
                     /locus_tag="PLY93_12635"
     CDS             19..>2251
                     /locus_tag="PLY93_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRP70372.1"
                     /translation="MQSVTKDATDKAKLILSTSTQQAQAYTLTILNLTSLIGTPITGV
                     IQIPFTGLSLPAATFTWRNPTDQSALPETIGSWTLCDPNVSSSACTVQPYYNKNAVDI
                     AVAGVDVAAYRYRLDAGSWGTEIPVATPLTLSGLSEGYHTLYIVVKHTLGYWQETTSP
                     DVKTFSWVQDTLPAADAAFDPVTLPVANGGTTASQSVNIRVIGSEIARYKICLDNGAY
                     NDCTTSTFQGATPTSVNTNVTIPNGDYTAILTPGAATFKVIGYDASGNAQATPVAAGT
                     FNYTIDTQNVEAVFKVSDLPSSINNGTTSATVNVLSTYGAVNYIGEIIDGTDCSSVDW
                     ATKAASPVSIATPITRSGMTDTVPQKTICAVGRSVGGLWQGSWDGTGTPPNITKYTWL
                     IDTTAPSVTIYWQNPIAAPTSTTQTTGYKFQVSSSGGASYYRHAVVSGAGTPCSSGVY
                     SANTSIETDISVDPPAILTSGTTVYKVCVVAGDLAGNWQSAASAAQTAEWTVDLEAPT
                     TNPSFSAASSSVRSFNTAALTFSIDNSTATADSYYYEVQVATDAAFTNIIADTTVKSC
                     KTSALPECPLALGIKDFTVAVDAFQSASYYARVKAGDALGNMHSSFSAASAEHFVVGK
                     IVGVIKNELNANVSGVAVKLLTSAGSDVTALYGGTITTDGSGAFTIDNVRTAKLGYQI
                     RVAEGDATYYPATKRNITVQEKGTAGLLQTNIGTLGVATKSGASPQTITAKVIDADDG
                     WMLG"
BASE COUNT          582 a          617 c          578 g          474 t
ORIGIN      
        1 gtcaacggcg gcttgcaagt gcagtcagta accaaagatg ccacagataa ggcaaagctt
       61 attttgtcga caagcacgca gcaagcgcaa gcgtacacgc tcacaatttt gaatttaaca
      121 agtcttatag gaactcccat aaccggcgta atccaaatcc cttttaccgg tttatctctg
      181 cctgccgcga cgttcacttg gcgcaatccc acagaccagt cggcgttgcc tgaaacgatt
      241 ggtagttgga ctttatgcga tcccaacgtt tcctcaagcg cgtgtaccgt gcaaccgtac
      301 tacaacaaaa atgccgtcga tattgcagtt gcaggtgtcg acgttgctgc ataccgctat
      361 agactcgacg cggggagctg gggcacagag attcccgttg ctacaccgct gacgctttcg
      421 gggttaagcg agggctacca cacgctctac attgtggtga agcacacatt ggggtattgg
      481 caagagacca cctcacccga cgtcaaaact ttctcgtggg tgcaagacac tctccccgct
      541 gccgatgcgg cgtttgatcc ggtgacactc cctgtggcca atgggggcac aaccgcttcg
      601 caaagcgtga atatccgcgt aattggttca gaaattgcgc gctataaaat ttgtttagac
      661 aacggcgcgt ataacgattg caccacaagc accttccaag gggcaacgcc gacttcggta
      721 aacacaaacg tcaccatacc caacggcgac tatacagcca tcttaacacc gggggcggcg
      781 actttcaagg tcattgggta cgacgccagc ggcaatgcgc aagcgactcc tgtagcagcg
      841 gggacattca attacaccat cgatacgcag aatgtcgaag cggtttttaa agtgtccgat
      901 ttacccagct ccatcaacaa tggcaccaca tctgcaacgg tgaatgtgtt gagcacttac
      961 ggcgcggtca actacattgg tgaaattatc gacggcaccg actgtagttc cgtcgactgg
     1021 gcaacaaaag cagcatcccc tgtgtctatt gcaacgccga ttacacgcag cggcatgacc
     1081 gatacagtcc cgcaaaaaac aatttgcgct gtggggcgta gcgtcggtgg tttgtggcaa
     1141 ggctcgtggg atggaaccgg gacgcccccc aatatcacaa aatacacttg gcttatcgac
     1201 accactgcgc cttcagtgac gatttactgg cagaacccga ttgccgcacc aacgtcaaca
     1261 acgcaaacga cggggtataa atttcaggtc tcctcgagcg gtggcgcgtc gtattaccga
     1321 catgccgttg tgagcggtgc aggtacaccg tgttcatcgg gtgtgtatag cgcaaacaca
     1381 tctattgaaa ccgacatcag cgtcgatcca ccggcgatac ttacgagcgg tacaacggtg
     1441 tataaagtct gcgtcgttgc cggagattta gcgggcaact ggcaaagcgc cgcctccgca
     1501 gcgcaaaccg cagaatggac ggtggattta gaagcgccca caacgaatcc ttcttttagc
     1561 gccgcttcga gtagcgtacg cagctttaac actgccgcct tgacgtttag tatcgataac
     1621 agtaccgcaa cggcagattc gtattactat gaagttcagg tggccaccga tgcagcgttt
     1681 accaatatta tcgccgacac tacggtaaaa agctgtaaaa cgagtgcact acccgaatgc
     1741 ccattggctt taggcataaa agatttcacg gtcgccgtcg acgctttcca atcggcaagc
     1801 tattacgccc gcgtgaaagc gggtgatgcg ttgggcaaca tgcacagcag tttttcggca
     1861 gcgtcggcag agcattttgt tgtcggtaaa attgtcggtg tcatcaaaaa cgaactaaac
     1921 gccaacgtta gcggcgttgc ggtcaaactc ctcacgagcg cggggagcga cgtcacagcg
     1981 ctttacggcg gcaccatcac aaccgatggt agtggcgctt tcacaatcga caacgtacgc
     2041 acagccaagt taggctacca aatccgtgtg gccgaaggcg atgcaacgta ctaccccgcg
     2101 acaaagcgga acatcaccgt ccaagaaaaa ggcaccgccg gtttgttaca aacaaacatt
     2161 ggtacattgg gggtcgccac aaaatccggc gcatcaccgc agacgataac ggcaaaagtg
     2221 attgacgccg acgatggttg gatgctcggc t
//
LOCUS       DAOMPD010000447         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_18998_length_2248_cov_3.196990, whole genome shotgun sequence.
ACCESSION   DAOMPD010000447 DAOMPD010000000
VERSION     DAOMPD010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18998_length_2248_cov_3.196990"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLY93_12640"
     CDS             <1..639
                     /locus_tag="PLY93_12640"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRP70373.1"
                     /translation="ILWAYSTHLYFSEGYLKKKRGVEAPFLTKTKVTSADALNKFALF
                     FHSYGGKGVESLVLRAEASKLTYLATFNEGKKQKKALMDAVTGNILSPIDEKFAVEIA
                     SQYVPQDTVFKSAEALDVYTHRKGRKVNNVYRVVFTGSETIEIFIDRSSGEIIEESSP
                     SRRFLFLMQKLHQLEFFGTKKELTIIPGASLLFLVITGILIWWRRFKRKNKF"
     gene            complement(685..1329)
                     /gene="rsmI"
                     /locus_tag="PLY93_12645"
     CDS             complement(685..1329)
                     /gene="rsmI"
                     /locus_tag="PLY93_12645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRP70374.1"
                     /translation="MPIGNAADIFARAHTTLSVVDFIACEDTRKTLELLRRARIETKA
                     RLLVHNPFGEAKSAEGLLKLLYEGKNIALVSDAGTPRLSDPGSRILRLAFTEKIPVVP
                     IPGAAALTTLLSITPFSVDPFVFLGFLSPKSGRRQNELKKYLDFEGAIALYESVHRIE
                     KCLADICDIYPDAEILIGREMTKTHEEFFLGSTLEATSWVQGKHGEFALIFRKK"
     gene            complement(1307..>2248)
                     /locus_tag="PLY93_12650"
     CDS             complement(1307..>2248)
                     /locus_tag="PLY93_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRP70375.1"
                     /translation="KNSGSPQFWNIKRFFQQPVSTKSLKHFVDRSRKYGLNTLVIDTQ
                     GKMLTREQISYIKSNGIYPIARVVCFDLGLKEKYPNKAHLDSIMQEVENAAKAGFQEV
                     QLDYIRYADEPKLLRLPLKFKYEQINHVLSRARRLTDSLGVELGADVFGRITLNEHDQ
                     IGQRLEIFGDHVHNLYPMVYPSHYYGDPEKIADPYGTVKEGVENSKERIPKTRIIPWI
                     QGFAMKIKESGLSLPEYILKQPYAVDDAKGDGYVVWNARNDYTSTWKAIKEYDRLVET
                     RKKHTPIQNSPVVRVNARRKKTYRYSLFGSSANWKRC"
BASE COUNT          613 a          469 c          505 g          661 t
ORIGIN      
        1 attttatggg cgtactccac gcacctttac tttagcgagg gctatctcaa gaaaaagcgt
       61 ggagttgaag cgcctttctt aactaagaca aaggtaacaa gcgcggacgc acttaataaa
      121 tttgctctgt ttttccatag ctatggggga aaaggggttg agtcgcttgt tttacgcgca
      181 gaagcttcga agcttaccta tttagctacg tttaacgaag gaaagaaaca gaaaaaggcg
      241 ttgatggacg ctgtaacagg gaatatttta agccccatcg atgaaaaatt tgcggttgaa
      301 attgcctcgc aatacgtgcc tcaagacaca gttttcaagt cagctgaggc tctcgatgtt
      361 tatacccacc gcaaaggccg caaagtaaac aatgtttatc gcgttgtgtt tacgggaagt
      421 gaaacgatag aaatttttat cgatcgtagt tcgggtgaaa ttatcgaaga gtcaagccca
      481 tcgcggcgtt ttctgtttct catgcaaaaa ttacaccagt tggagttttt tgggacaaaa
      541 aaagaactga cgataattcc tggagcctct ctccttttct tggttattac aggaattctt
      601 atttggtggc gtagatttaa aagaaaaaat aaattttaac cactctcact ataaaataaa
      661 agacgaagtt ttttaagaaa ctttttattt tttacgaaaa atgagtgcaa actccccgtg
      721 tttaccctgt acccaagaag ttgcttccag agtgctacct aaaaaaaatt cttcgtgcgt
      781 tttagtcatt tcacgaccaa tgagaatttc tgcatcgggg tagatgtcac aaatatcggc
      841 aaggcatttt tcaatacggt gtaccgattc gtagagcgca attgctcctt caaaatctaa
      901 atatttctta agttcgtttt gccgcctccc gcttttggga gaaagaaatc ccaaaaacac
      961 aaaagggtca acggagaaag gggtgatact taaaagcgtt gtgagtgctg ccgcgccagg
     1021 aataggcacg actggaattt tttcagtaaa agctaagcgc agtatacggg aacctgggtc
     1081 agaaagccgc ggcgttcctg catccgaaac cagcgcaata tttttccctt cgtatagaag
     1141 ctttaacagc ccttcggcgc tttttgcttc gccaaaaggg ttgtgtacca aaagccgcgc
     1201 tttagtttca attcgcgcac ggcgcaatag ctccaaggtt ttgcgcgtat cttcacaggc
     1261 gataaaatcc acaacagata aggtcgtgtg cgcacgcgca aaaatatcag cagcgtttcc
     1321 aattggcact gctaccgaat aaagagtacc tgtatgtttt ttttcttctg gcattaaccc
     1381 gaactacggg agagttttga atgggggtgt gttttttccg cgtctcaact aagcggtcgt
     1441 attctttaat tgctttccat gtcgatgtgt agtcgttacg cgcattccac acgacatagc
     1501 catcgccctt ggcgtcatcg actgcgtaag gttgcttcaa tatgtactcg ggtaaagaaa
     1561 gccctgattc tttaattttc atcgcaaaac cttgtatcca cggaatgata cgcgtttttg
     1621 gaatgcgctc tttggagttt tcgacgcctt ctttaactgt accgtaagga tcagctattt
     1681 tttcagggtc gccgtagtaa tgcgaagggt ataccatggg gtagaggttg tgtacgtgat
     1741 cgccaaagat ttcaagcctt tgcccaattt ggtcgtgctc gtttaacgta atgcgcccaa
     1801 aaacatccgc gcctaattcg acccctaaag aatcggttag gcggcgcgca cggcttaaga
     1861 cgtggttgat ttgttcgtat ttaaatttta gtggaaggcg taatagtttt ggctcatccg
     1921 cgtaccggat gtagtcgagt tgcacttctt gaaaccccgc tttagctgcg ttttcaacct
     1981 cttgcattat gctatctaaa tgcgctttat taggatattt ttctttaagg ccgagatcaa
     2041 aacagacaac acgcgcaatg ggataaattc cattcgactt gatataagaa atttgttcgc
     2101 gggtgagcat tttaccctgc gtgtctatta caagcgtgtt gagaccgtat ttgcggctac
     2161 ggtcgacaaa gtgtttaagc gattttgtac taacaggctg ttgaaaaaaa cgcttaatat
     2221 tccaaaattg gggactgcct gaattttt
//
LOCUS       DAOMPD010000448         2242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19050_length_2242_cov_2.549154, whole genome shotgun sequence.
ACCESSION   DAOMPD010000448 DAOMPD010000000
VERSION     DAOMPD010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19050_length_2242_cov_2.549154"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1064)
                     /locus_tag="PLY93_12655"
     CDS             complement(132..1064)
                     /locus_tag="PLY93_12655"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRP70376.1"
                     /translation="MKIDAHTEIFGIIGHPLGHTFSPTMHNAAFAAKKINAVYLAFPI
                     KNLLRLKSSMRQWNIRGLSVTIPHKISIRRKLDRIDPLALQIGSVNTILWGKTGLLEG
                     YNTDGPGAMMALKKARVPLLDKKILILGSGGSARGIAFALTREKPQEIAIMARNPTMA
                     MQLARNLTLQKENPQVTLLINEKRNQSRLTDFMPQHGRKWTSLYCSTPEQIEKYDLII
                     NTTPVGMRGSENQKHSPLTIHELKKHQTVFDIVYNPSETPLLKLARKKGCGIVMGYKM
                     LLYQGVLQFELFTGQSAPVQAMEKALIEEIKRIS"
     gene            complement(1061..1444)
                     /locus_tag="PLY93_12660"
     CDS             complement(1061..1444)
                     /locus_tag="PLY93_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70377.1"
                     /translation="MLVEVRRELVKQDHRYLQKRFLQDAHNRLAIYIWQLPDDAADEM
                     LRFQISYREIALDWAAERGIRYASVDDGENPMGMKRSPVLKQEHSVRESLLTKLIDLL
                     KGYSDKKSLQFIIDIIENESIVKKI"
     gene            complement(1450..>2242)
                     /locus_tag="PLY93_12665"
     CDS             complement(1450..>2242)
                     /locus_tag="PLY93_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRP70378.1"
                     /translation="LQRKGATLMKAEKLSIPASAAFTLLFIATIIVGFSINNSSLSAA
                     PKRQLGETQKEAELREQLFNEFYRQWKAGEKLGLDTPKTGSKGAAHPTITVVEFADPL
                     CPHCKSMGIVLDAFVKANAAKVRVIYRHYPLDIQCNSAMRQAFHQGACDLARAMECGA
                     AQDKFWPVHDAVFQEQETFFRNPVTDKAIESIGQNAGVQVSLFAQCFKSQATMAKVKS
                     DIAAGNRMKITGTPTVVINDRRLPSIPLEYISGVLEKILLEESKR"
BASE COUNT          546 a          512 c          523 g          661 t
ORIGIN      
        1 aagcggcgtg caatcgccga tttacctacg ccacgaaaac cgatgagcgc aatacgcggt
       61 tttttcggcg tttgtactct ttcgcgtcga tgctttttat gatgagcgtt tttatttttt
      121 tgactataca tctatgaaat ccgtttaatc tcttctataa gagctttctc catcgcttga
      181 accggcgcgc tttgtccagt aaaaagttca aattgcaaca cgccctggta gagaagcatt
      241 ttgtaaccca tgacaatgcc acaacctttt ttacgcgcca atttaagcag cggcgtttcg
      301 ctgggattgt aaacgatatc gaataccgtt tggtgttttt ttaattcatg tatggtaagg
      361 ggactgtgct tttgattttc tgaaccacgc atgccaacag gggtggtatt gataatcaaa
      421 tcgtattttt caatttgttc gggggtacta caatatagac tcgtccactt gcgcccgtgc
      481 tgcggcataa agtcggtgag gcggctttga ttccgttttt cgttgataag gagcgttact
      541 tgcggatttt ctttttgcaa agttaaattc cgcgcaagct gcatcgccat ggtagggttg
      601 cgcgccataa tagcaatttc ttgcggcttt tcgcgcgtga gagcaaaagc aatcccccgt
      661 gccgatccac ccgaacccaa aatgagaatt tttttatcga ggagcggaac gcgcgctttt
      721 ttaagcgcca tcattgcgcc tggaccgtcg gtgttatagc cttctaaaag accagtcttt
      781 ccccaaagga tggtattgac tgaaccaatt tgtagagcca acggatcgat acggtcgagc
      841 ttgcggcgga tggaaatttt atgcgggatt gtcaccgaca gcccacggat attccactgg
      901 cgcatcgacg atttaagccg caataaattt tttatcggga atgccaaata caccgcgttt
      961 atttttttag ccgcaaacgc cgcgttgtgc atcgtcggtg aaaaagtatg acctaaagga
     1021 tggccaataa taccaaaaat ttctgtatgc gcgtcaattt tcatattttt tttacaatgc
     1081 tttcgttttc tataatatca ataataaatt gcagcgattt tttatcggag tatcctttca
     1141 aaagatcaat gagttttgtt aagagagatt cacgcactga atgctcttgc ttcaaaacag
     1201 gagaacgttt catccccatc ggattttcac cgtcgtcgac gctcgcataa cgtatacctc
     1261 gctcggccgc ccaatcgagc gcaatctcgc ggtatgaaat ttgaaagcgt agcatttcat
     1321 cggcggcatc atcggggagt tgccaaatat aaatagcaag tctattgtgt gcgtcttgca
     1381 aaaatcgttt ttgtaagtag cggtggtctt gcttgacgag ttctcgtcgc acctcgacaa
     1441 gcattctact taccgcttcg attcttcgag tagaattttc tctaaaacgc ctgaaatata
     1501 ttctaatgga atcgatggaa gacgacggtc gttaataaca accgtgggcg taccggtaat
     1561 tttcatgcgg ttaccggcag cgatgtcgct cttcacttta gccatcgttg cttgcgactt
     1621 gaaacactgc gcgaaaagcg aaacttgaac gcccgcattc tggccgatac tttcaatcgc
     1681 tttgtcggtc acgggattac gaaagaaagt ttcttgttct tgaaagacgg catcgtgtac
     1741 cggccagaac ttatcttggg ctgcgccaca ctccatcgca cgcgctaaat cgcacgcccc
     1801 ttggtggaac gcttggcgca tggcgctatt gcattggatg tcgagtggat agtggcggta
     1861 aatcacacgt acttttgcgg catttgcttt cacgaacgca tcgagaacaa tccccatact
     1921 cttgcagtgc gggcaaagag ggtcggcaaa ttcaacgacg gtgattgtgg ggtgtgcggc
     1981 tcccttgctg cctgtcttgg gggtatctaa accgagcttt tcgcccgctt tccattgtct
     2041 atagaactcg ttaaaaagtt gttcgcgcaa ttcggcttct ttttgagtct cacccaactg
     2101 ccgtttaggg gctgctgaaa gcgagctgtt gttgatagag aagccaacga taatagttgc
     2161 aataaaaaga agagtaaatg cagcgcttgc ggggatagac aatttttctg ccttcattag
     2221 agttgctcct ttccgttgta at
//
LOCUS       DAOMPD010000449         2241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19067_length_2241_cov_2.316102, whole genome shotgun sequence.
ACCESSION   DAOMPD010000449 DAOMPD010000000
VERSION     DAOMPD010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19067_length_2241_cov_2.316102"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLY93_12670"
     CDS             <1..416
                     /locus_tag="PLY93_12670"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP70379.1"
                     /translation="EFITLFSLELHITKGAITLNYINAGQHPVLMYQHDKLIRLEDSQ
                     KLLGVTKAEYKTTTLEFDASPMRLILYSDGAFDVFSKAGKHLGHKKFYEWVESSLAKS
                     PADQLTFLRQKIEAYTGSGEESDDLAIIIIETKQS"
     gene            complement(413..1690)
                     /locus_tag="PLY93_12675"
     CDS             complement(413..1690)
                     /locus_tag="PLY93_12675"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRP70380.1"
                     /translation="MATQSSLNLREKLIKFGTWIFLALLFVIIVLSFGMPDFIGTSSR
                     MDSYNAAKIGGEYLTRAEVAESQKQIEERMAQNMKGLDAKNRKILEDMAKGRALDESI
                     DRKIFTQVLKEEGYIPSASSEARILATFYKRQFGEYIINGKLDIERLNEVLGKRRLSL
                     DQIGRTILLDYGPQKAFEMLQATAYASPYTLIDAARFATTRNSLRVVVLDSATKDKLM
                     RARFNPSEAEIQAKFKTEFLARDAKAVLDKVKRDSIRATLFNENRANLEKDLTTYLQN
                     TASKGIDAIAAATGASPLLLTDVGLSDDLDAKKPKGLSTSLSPLSQSEVFVKQRLGAP
                     IGHLIGPVDAGGLTYFFTITARKEANLPAATEYKKIENAKEILTKAKDLPKGMTYDTL
                     FESAARGNYGELLTAAVEMQRNSVRIIRYNTAN"
     gene            1781..>2241
                     /locus_tag="PLY93_12680"
     CDS             1781..>2241
                     /locus_tag="PLY93_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HRP70381.1"
                     /translation="MNSYITQIADKALNVSATLRRLSSAQKNAILEKLAQLIEERAPA
                     LKTENAKDIAYAKEIKLEASLVERLTLSDKVIEAMARAAREIAALPDPVGEVIEGRTL
                     ESGLKLTKVRAPLGVILLIYESRPNVTIDVGALCLKSGNAAILRGGKEAIH"
BASE COUNT          562 a          516 c          555 g          608 t
ORIGIN      
        1 atgaattcat tactcttttt tcgttagaac tacacattac aaaaggagcg attacgctca
       61 actatatcaa cgctggtcag catccggtgc tcatgtatca gcacgataag ctcattcggc
      121 ttgaagatag tcaaaagctt ttaggcgtca ctaaagcaga gtacaaaact acaacgcttg
      181 aattcgacgc ttcacccatg cggctaattc tttattccga cggcgcgttc gatgtgttta
      241 gtaaagcggg caagcatttg gggcataaaa agttttacga atgggttgaa tcgtcgctgg
      301 caaaatcccc cgcagatcaa cttactttct tgcggcaaaa gatagaagcg tacaccggtt
      361 cgggtgaaga aagtgacgat ttagcgatta ttattatcga aacaaaacag agttaatttg
      421 cagtgttgta tcgaatgatg cgcacgctat tgcgttgcat ttcaaccgcc gctgtcaata
      481 gttcaccata gttcccccgt gcggcgcttt caaaaagtgt gtcgtaagtc atacccttgg
      541 gcaaatcttt tgctttggta agtatttctt tggcgttttc aatttttttg tattcagtag
      601 cagcgggcaa gttcgcttct ttgcgagcgg tgattgtaaa gaagtaagtc aatccacccg
      661 catcaacggg gcctatcaag tgaccgatgg gtgcacctaa gcgttgtttg acaaacactt
      721 cggattgcga aagtggcgag agcgaagtag agagcccttt gggctttttg gcgtctaaat
      781 cgtcgctcaa ccccacgtcg gtaagtagaa ggggggatgc acccgttgca gcagcgatgg
      841 cgtcgatacc tttggatgca gtattttgta aatacgtcgt taaatctttt tctaagttcg
      901 cgcggttttc gttaaagaga gttgcacgga tactgtcgcg tttaacttta tcgagcaccg
      961 ctttggcatc tctcgccaaa aattcggtct tgaattttgc ttggatttca gcttcggatg
     1021 gattaaaacg cgcacgcatg agtttgtctt tggtggcgct atctaaaacc accacgcgca
     1081 atgaatttcg tgtggtggca aaacgcgccg catcgatgag ggtgtacggc gatgcgtacg
     1141 cagtggcttg cagcatttca aacgcttttt gtgggccgta gtccaaaagg atagtgcggc
     1201 caatttggtc gagggataag cgtcgtttgc ccaaaacttc gttgaggcgt tcgatgtcga
     1261 gtttaccgtt gatgatgtat tcgccaaatt ggcgtttata gaacgtcgct aagatgcgcg
     1321 cttcgctcga cgccgaaggg atatacccct cttctttgag tacttgcgta aaaattttac
     1381 ggtctatcga ctcgtcgagt gcgcggcctt tcgccatatc ttcgaggatt ttacggtttt
     1441 tagcatcgag acctttcatg ttttgtgcca tgcgctcctc gatttgtttt tggctctcgg
     1501 ccacctctgc acgggtgagg tattcgccgc caattttcgc cgcgttgtac gaatccatgc
     1561 gcgagctggt accgataaaa tcgggcatac caaaagaaag cacgatgata acaaaaagaa
     1621 gcgcgagaaa aatccatgta ccaaatttga tgagtttttc acgcagattg agcgacgatt
     1681 gagtggccat ttgccaacat cgatttttga tttacctttt caaacctata ttcaagtttt
     1741 agggttgtcg cacagactct aaaagcaact attttatcgg atgaactcgt atatcactca
     1801 aattgccgac aaagcgctca atgtttccgc cacgctgcgc cggctgtcga gcgcgcaaaa
     1861 aaatgctatc cttgaaaaac tggcgcagct tatcgaagag cgtgcgccag cgcttaaaac
     1921 cgaaaacgcc aaagatattg cgtatgcgaa agaaataaaa ctagaagcga gccttgtcga
     1981 acgcctcact ctttcggata aagtcatcga agcgatggcg cgtgccgccc gcgaaattgc
     2041 cgcgcttccc gatccggtag gtgaggtgat tgaagggcgc actcttgaaa gcgggcttaa
     2101 actcaccaag gtgcgtgcgc ctttgggtgt cattctactc atctacgaat cgcgccccaa
     2161 tgtgaccatc gacgtgggcg cactttgtct caaatcgggg aacgcggcaa ttttgcgcgg
     2221 cggcaaagag gccatccaca g
//
LOCUS       DAOMPD010000450         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19177_length_2231_cov_2.189798, whole genome shotgun sequence.
ACCESSION   DAOMPD010000450 DAOMPD010000000
VERSION     DAOMPD010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19177_length_2231_cov_2.189798"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PLY93_12685"
     CDS             <1..1449
                     /locus_tag="PLY93_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRP70382.1"
                     /translation="IDGDENNNNLGDWQAGGGRLHAKTIAVDAGTPNAKLMLGSFNWS
                     PNANNNNDENLIIIHSPAIVERYLKFWQQIYDDGVPMDQRYPTNAEMAAGITTYQKRE
                     RGDYQKIIISEVNYAGTTRKIDGKFYAYDSNQFIELYNPNDRAIDISFWSLYFPVIDN
                     YTDPGVYSSGWPGYEALAKRGLVGFPGGTVIPAKGFVVVTKPDQPKDLNTDGYSFPAS
                     VLYDPANAASFSNKITYYNPANGLSNFFTLFDRRTSSTGRTTVVGGGPLTFEFPAFDN
                     TTGKCRPAFNAEIDRFTGSGYFNPYHMHAGNTAGYPHFTSLWIELRDNRGNLVDIAGH
                     QRTNNGAAYTLGVSSAPTTNADVGYLKGGFFTAASSGNTTDGTRLVDSGSISFPNNGL
                     PLGTGTAASDRICDIQATNRLYMASMERNMPGGGVWGDGTNPNSWDHATKDIAGGGSG
                     GNGGTGAYIKSDYKDYTVATPGEINSRWSALP"
     gene            1458..>2231
                     /locus_tag="PLY93_12690"
     CDS             1458..>2231
                     /locus_tag="PLY93_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70383.1"
                     /translation="MNGPQYFRNRAKTRYSACLLCLFLSCTSGSQFDERNQDFFKDAK
                     DAEKINYTTEPLTFSGGIQTLITTISGFGLNIETPIPATIEGIVTMPSNYAVAVGATA
                     TGCANNDVIYSRSFILQDKDAAILVVYGLNPPSQNTADAANAKYILNAKNDGMASLGN
                     KLKITVTKAQKYGGGANVIPIVTDFDVTSRVVLSSGNAIPYATQSSAFARGVDLYQTR
                     QLEGYVLQSPAYVECSSGRSFQFNYQQGYMGILCVGAASY"
BASE COUNT          593 a          534 c          573 g          531 t
ORIGIN      
        1 atcgacggcg acgagaataa caacaatttg ggtgactggc aagcgggcgg ggggcgtctc
       61 catgcaaaga cgattgccgt cgacgcgggc actcccaacg caaagctgat gctcggctct
      121 ttcaactggt cgccgaatgc caacaacaac aacgatgaaa atctaatcat cattcattca
      181 ccggcgattg tggagcgata tttaaaattt tggcagcaaa tttacgacga tggcgtgcca
      241 atggatcagc gttatcccac caacgctgag atggcggcgg ggataactac ctatcaaaaa
      301 cgcgagaggg gtgattatca aaaaattatt atttccgagg tgaactacgc gggcaccacc
      361 cgtaaaatag atggtaaatt ctacgcgtac gacagcaacc aattcattga actctataac
      421 cccaacgacc gtgcgattga tatttcattt tggagtctct attttccggt aattgataac
      481 tacaccgacc cgggtgtgta ttcgagcggt tggcctgggt atgaagcgct cgccaagcgc
      541 ggccttgtgg gatttccggg cggtacagtt atccctgcga aaggctttgt cgttgttacc
      601 aaacccgacc agccgaaaga cttgaacacc gatggatata gttttcctgc aagcgtactt
      661 tatgaccccg ccaatgcggc gtcgtttagt aacaagatta catactataa ccccgccaac
      721 ggcctctcga atttttttac gctttttgat cggcgtacca gcagtacggg gcgtacaaca
      781 gtggtagggg gtggcccact cacctttgag ttcccagcgt ttgataatac aaccggcaaa
      841 tgcagacctg cttttaacgc agagattgac cgctttaccg gttcgggata ttttaaccca
      901 taccacatgc acgcagggaa caccgcaggc tatccacatt tcacttcgct ttggattgag
      961 ctgcgcgaca accgaggcaa cttggtcgac attgcaggcc accaacgtac aaataatggc
     1021 gccgcatata ccttaggcgt ttcgagtgcc ccaaccacga atgcggatgt gggctacctc
     1081 aaaggcggat tttttactgc agcatcgtcg gggaacacaa ccgatggcac tcggcttgta
     1141 gattcaggga gcattagctt tcccaataac ggattacctt taggtacagg cactgcggct
     1201 tcggatcgga tttgcgacat ccaagccacc aaccgcctct acatggcgtc gatggaacgt
     1261 aacatgccag gtggaggcgt ttggggtgat ggtaccaatc ccaatagctg ggatcacgcc
     1321 acgaaagaca ttgccggtgg cggtagcggt ggtaatggcg gtacgggtgc gtacataaaa
     1381 tcggattaca aagactatac tgtcgccact ccgggggaaa tcaattcgcg ctggagcgcg
     1441 ttgccgtagg aggaattatg aatgggccac aatattttcg taacagagca aagacgcgct
     1501 atagcgcatg cttactctgt ttatttttat cgtgtacctc gggcagtcaa ttcgacgagc
     1561 gtaatcaaga tttctttaag gatgcgaaag acgcagagaa aatcaactac acgacagagc
     1621 ctcttacctt ttccggtggg attcagacgt tgataacaac aatcagtggt tttggtctaa
     1681 atatcgagac gccaataccg gcgaccatcg agggcattgt cacgatgccg tctaattatg
     1741 cggtcgcggt gggggcaacg gcgacggggt gtgcgaacaa cgatgttatt tattcacgct
     1801 cgtttattct acaagataaa gacgcagcaa tccttgttgt gtacggactc aatcccccca
     1861 gccaaaacac agcggatgct gcgaacgcaa aatatatctt gaatgccaaa aacgacggta
     1921 tggcgtcttt ggggaataag ctaaaaatta cagtcaccaa agcgcaaaaa tacggtggtg
     1981 gcgcaaacgt tatacccatt gtgactgatt tcgatgtaac aagtcgtgtt gtgttgtcat
     2041 cggggaatgc aatcccctat gcaacacaaa gtagcgcatt tgcaagggga gtggatttat
     2101 accaaacacg acaattagaa gggtacgtgc tccagtcgcc ggcgtatgta gaatgttcgt
     2161 cgggacgctc gttccagttt aattaccaac agggttatat ggggattttg tgtgtcggtg
     2221 cggcaagtta t
//
LOCUS       DAOMPD010000451         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19223_length_2226_cov_2.467526, whole genome shotgun sequence.
ACCESSION   DAOMPD010000451 DAOMPD010000000
VERSION     DAOMPD010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19223_length_2226_cov_2.467526"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLY93_12695"
     CDS             complement(<1..260)
                     /locus_tag="PLY93_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRP70384.1"
                     /translation="MKNIAIIAHVDHGKTTLVDKLLGFCSTLEAKESTERVMDSNDLE
                     RERGITILAKNTAVEYEGTRINIVDTPGHSDFGGEVERVLKMV"
     gene            complement(310..867)
                     /locus_tag="PLY93_12700"
     CDS             complement(310..867)
                     /locus_tag="PLY93_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRP70385.1"
                     /translation="MITSVSDSSTVVTAELSVEALFLKHKTQLLRYLHNFVGNDEADD
                     VLQDVFISFLAQKRAEKIRATDELGWLYRACHNRAIDYIRKRKKLIYFTEEKFDRMEA
                     TEENKNTRTWTELRDTLHTLALRFGKKGEGILLLHLLEEGKPKILIAEIMEITDRHLR
                     RRIESFYQFLQQELKKLGIEKIELE"
     gene            complement(877..1614)
                     /locus_tag="PLY93_12705"
     CDS             complement(877..1614)
                     /locus_tag="PLY93_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRP70386.1"
                     /translation="MRLIDALLKRALAESEKKALGMILAGQVLVNDTPVTKPATPVSA
                     QDTIRVRGIKKYVSRAGEKLAGALDAFMFPVENRSFIDIGASTGGFTDVLISRGAAYV
                     AAVDVGKGLLHQKLRLNPRVEIFEGVDFKNFGAERLHQKPEAFVADVSFTSLNLIVEK
                     AFSLLKPVHRKVEGVVLFKPQFELPKAERNLLDKGVLHNEKRLKQLLHDFETQMQSLN
                     IIIQALIPAAVKGAKGNQEYVAHLTRP"
     gene            complement(1633..>2226)
                     /locus_tag="PLY93_12710"
     CDS             complement(1633..>2226)
                     /locus_tag="PLY93_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRP70387.1"
                     /translation="DLPAMDDDDFRRGQPSSHKKYGEATAILVGDLLQALSFEFLAMA
                     RLNPKAMVYFARAVGTQGMVAGQFMDMQASSSNTLRMHQLKTGRLIEAAVVLPFFIGE
                     NKIPLSVVRWARALGLLFQITDDLLDGTQSSSTLGKTAGKDLAQNKKTIVALLGQSEA
                     EKRAALLSTRLENQAKQIFTASAFFASLPSFLLHRKS"
BASE COUNT          525 a          510 c          562 g          629 t
ORIGIN      
        1 accatcttta gcacacgctc gacttcgccg ccaaaatcgg agtgaccggg ggtgtcgaca
       61 atattgatac gcgtaccttc gtattcaacg gcggtgttct ttgctaaaat ggtgatgccg
      121 cgttcgcgtt ccaggtcgtt ggagtccatc acgcgttcgg tgctttcttt tgcttccagt
      181 gtggagcaga agcccaagag tttgtcgaca agagttgttt tgccgtggtc gacgtgggca
      241 atgatggcga tgtttttcat tggagtattt tcaataccga aagatgagat ttagacgagg
      301 tgtaaagtct tactccagct ctattttttc aatccccaac ttctttagtt cttgttgtaa
      361 aaattgataa aacgattcga tacgccgccg taagtgtcga tcagtaattt ccataatctc
      421 tgcgataaga attttcggtt ttccttcttc gagtaaatgt agcaacaaaa taccttcgcc
      481 ctttttacca aatcgaagcg caagcgtgtg gagcgtatcg cgtagctctg tccaagtgcg
      541 ggtattctta ttttcttcag tggcttccat gcggtcgaat ttttcttcgg taaaatagat
      601 aagctttttg cgtttgcgaa tatagtcgat ggcgcggtta tggcaagcac ggtagagcca
      661 gcctaactcg tcggtagcgc ggattttttc ggcgcgcttt tgcgccaaaa aagaaataaa
      721 tacatcttgg agtacgtcgt ccgcttcgtc gttgccgaca aaattatgaa ggtagcggag
      781 cagttgcgtt ttatgtttta agaagagcgc ttcaacgctc aactcagccg taacgaccgt
      841 ggatgaatcc gatacagaag taatcatagt ggagtgttat ggacgtgtca aatgggcgac
      901 gtattcctga ttacccttgg cgcctttgac ggcagccggt ataagcgctt ggattatgat
      961 attcaaagat tgcatttgtg tttcaaagtc gtgtaacaac tgtttaaggc gtttttcgtt
     1021 gtgcaagacg cctttgtcaa gcagattgcg ttctgctttg gggagttcaa actgtggttt
     1081 gaaaagcacg acaccttcta ccttgcggtg aaccggtttt aagagtgaga atgccttttc
     1141 tactattaaa ttaagcgagg taaaagagac atctgccaca aatgcttcgg gtttttggtg
     1201 gagtcgctca gcgccaaagt ttttgaagtc gacgccttca aaaatttcaa cgcgggggtt
     1261 aaggcgcagc ttttgatgga ggagtccttt ccccacatcc actgccgcta catacgctgc
     1321 gcctcgggaa atgagcacgt cggtaaagcc gccggtcgat gcaccaatgt cgataaagct
     1381 gcggttttcc acaggaaaca taaaagcatc gagcgcaccg gctaattttt cccctgcacg
     1441 cgagacatat ttttttatcc cccgcacacg gatggtgtct tgtgcggaca cgggtgttgc
     1501 aggttttgtc accggtgtat cgtttaccaa cacttggcca gcgagaatca tccccaaggc
     1561 ttttttctcg ctctctgcaa gggcacgttt aaggagggca tcaatcagtc tcatcgacgc
     1621 cgaacctgtg ggttatgatt tacggtggag caagaacgaa ggcagcgatg caaaaaaagc
     1681 ggaggcggta aaaatttgtt ttgcttgatt ttcaagtcga gtggaaagga gggcagcacg
     1741 cttttcagct tcgctttggc ctaaaagcgc gacaatcgtt tttttatttt gcgcaaggtc
     1801 tttaccggcg gttttcccta acgtcgatga actctgtgta ccatcgagga ggtcgtcggt
     1861 gatttggaac aggagtccca acgcgcgcgc ccagcgcact accgacagcg ggattttatt
     1921 ttcgccgata aaaaagggga gtaccaccgc tgcttcgata agtcgtcctg tttttaactg
     1981 gtgcatccgc agtgtgttgc tgcttgatgc ttgcatatcc ataaactggc ctgccaccat
     2041 gccttgtgtg cccaccgcgc gggcaaaata aaccattgct ttgggattta agcgagccat
     2101 cgccaaaaat tcaaaactga gcgcttgcaa taaatctccc acgaggattg ccgtcgcctc
     2161 gccgtatttt ttatgactcg aaggttgccc gcgtcgaaaa tcgtcgtcgt ccattgcggg
     2221 caagtc
//
LOCUS       DAOMPD010000452         2224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19243_length_2224_cov_2.642231, whole genome shotgun sequence.
ACCESSION   DAOMPD010000452 DAOMPD010000000
VERSION     DAOMPD010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19243_length_2224_cov_2.642231"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /gene="ccsA"
                     /locus_tag="PLY93_12715"
     CDS             complement(<1..261)
                     /gene="ccsA"
                     /locus_tag="PLY93_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRP70388.1"
                     /translation="MYYALWVSAFALTAYSTYVALLQAPADAIQGNVQRIFYFHVPLV
                     WVSFIAFVGGAVMSFRYLLSRNMRHDTWARAFIKTGWAFTTTV"
     gene            complement(274..1314)
                     /gene="mtnA"
                     /locus_tag="PLY93_12720"
     CDS             complement(274..1314)
                     /gene="mtnA"
                     /locus_tag="PLY93_12720"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRP70389.1"
                     /translation="MKTNLSFIKTARDKIQLVDQKLLPQKLDYVTCKNGTDVAKAIKE
                     MVVRGAPAIGISAAYGMYLTAWEASKKNKKLTVDIFKKQKQQLDAARPTAVNLMWATS
                     EMLKTAEVFLTSDAAKAKDAALKLLLKLYDKALEIHNDDAERCLTMSNHGVKYLLTKY
                     PKEKHNILTHCNTGSLATGGIGTALGVIRLLSEKGKINMVYADETRPYMQGARLTVFE
                     LQKDKIPVTLTIDSQAAYLMQKKMVDAVFVGADRIAANGDVANKIGTYSLSVLAKAHR
                     IPFFVVAPQSTFDKTIETGNDIVVEERSAKEVLEIQGKQIAPKVPVVNYSFDVTPQAN
                     IAAIFSEDGVLE"
     gene            complement(1330..>2224)
                     /locus_tag="PLY93_12725"
     CDS             complement(1330..>2224)
                     /locus_tag="PLY93_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRP70390.1"
                     /translation="AIASGAAARPFVTHHNALDIELYMRIAPELYLKRLIVGGYEKVF
                     ELNRNFRNEGVSTKHNPEFTMIEIYQAYADYHTMMDLVQDIFEMLALKLLGTTEIPYG
                     EHVISLKKPWKRMEYLESIKENTGLDFAAFLHEENPSVDAAKKMAASKGLKAEALNTF
                     WEVVDLVFSEKVEPTLIQPTIIMHFPKAISPLAKSIPGNPYLVERFEPYITGREMGNA
                     FSELNDPLEQELRFNEQASQRDSGAEETMATDNDFVTALKVGMPPTGGLGIGIDRLVM
                     LLTNKASIKDVILFPLLRPQK"
BASE COUNT          513 a          547 c          529 g          635 t
ORIGIN      
        1 tacagtcgtg gtgaacgccc agcccgtttt gatgaacgca cgtgcccacg tatcgtgacg
       61 catgttgcgc gagagaagat aacgaaagct catcaccgct ccgcccacaa aggcgataaa
      121 cgaaacccag acaagcggga catggaaata aaaaatacgc tgcacattac cttggattgc
      181 gtcggcgggg gcctgcaaga gcgcgacgta ggttgagtat gccgttaagg cgaatgccga
      241 aacccaaagt gcgtaataca tctctctaac cttttactcc aacacgccgt cttcggaaaa
      301 aatagcggcg atgttcgctt gcggcgtcac atcgaaactg taattgacga cgggcacttt
      361 gggtgcaatt tgctttcctt gaatttcgag cacttctttg gcgctgcgct cttcaacaac
      421 aatgtcgttg ccggtttcaa tcgtcttatc gaaagtcgat tgcggagcaa caacaaagaa
      481 tgggatacgg tgcgcctttg ccaacaccga aagcgagtat gtgccgattt tattcgccac
      541 gtcgccattc gcggcaatac ggtctgcgcc cacgaacacg gcatcgacca tttttttctg
      601 catgaggtac gccgcttgcg aatcgatggt gagagtcacc ggaattttat ctttttgtaa
      661 ttcaaaaacg gtgagtcgcg ccccttgcat atatggccgt gtctcgtcgg cgtacaccat
      721 gttgattttt cctttttcgc tgagcaagcg aataactccc aacgcagtgc cgatgccacc
      781 ggtcgctaat gaaccggtgt tgcagtgagt gaggatgttg tgtttttcct tggggtattt
      841 tgtgagtaga tacttcaccc cgtggttcga catcgtgagg caacgttcgg catcatcgtt
      901 gtggatttcc aatgccttgt cgtataactt gagcaataat ttaagcgctg cgtcctttgc
      961 cttcgccgca tcgctcgtta aaaacacctc tgctgttttg agcatctcgc tcgtcgccca
     1021 cattaagttt accgctgtgg ggcgtgccgc gtcgagttgc tgtttttgtt ttttgaatat
     1081 atccacagtg agctttttat tttttttcga cgcttcccat gcggttaaat acataccgta
     1141 cgctgcgcta atgccgatag cgggcgcacc acgcaccacc atttctttaa ttgccttagc
     1201 aacgtcggta ccatttttac acgtcacata atccaatttt tgcggtaaca gtttttgatc
     1261 gacgagttgg attttatcgc gtgctgtttt tataaatgag aggttggtct tcatactttt
     1321 ctctaaaaat tatttttgcg gtcttaacaa aggaaataag attacatctt taatactcgc
     1381 tttatttgtc aacagcatca ccaagcggtc gatgccaatg cccaacccac cggtgggagg
     1441 catgccgact ttgagtgcag tcacaaagtc attgtcggtc gccattgtct cttccgctcc
     1501 gctgtcgcgc tgcgacgctt gctcgttaaa acgcaactct tgttccaagg gatcgttgag
     1561 ttccgaaaaa gcattcccca tttcgcggcc agtgatgtat ggctcaaagc gttcgacgag
     1621 gtacggattt cccgggattg atttcgccaa aggcgatatt gctttaggga agtgcatgat
     1681 aatcgttggt tggatgagcg tcggttctac tttttcggaa aaaacaagat cgaccacttc
     1741 ccaaaatgtg ttgagcgctt ctgctttaag tccttttgac gccgccattt tctttgccgc
     1801 atccaccgac ggattttctt cgtggaggaa tgcagcaaag tcaaggccgg tattttcttt
     1861 tatactctct aaatactcca ttctcttcca cggttttttc aacgagatga cgtgctctcc
     1921 ataaggaatt tctgtggtgc ctaaaagttt gagcgcgagc atctcaaaaa tatcttgtac
     1981 taagtccatc atcgtgtggt aatccgcgta cgcttgataa atttcaatca tagtgaactc
     2041 ggggttgtgc ttggtgctca cgccctcgtt gcggaaattg cggttgagtt cgaacacttt
     2101 ttcatagcca ccgacgataa gacgtttcaa atagagctcg ggcgcaatgc gcatatagag
     2161 ctcaatgtcg agcgcgttgt gatgcgtaac gaaaggccgc gccgccgccc ctgaggcaat
     2221 tgct
//
LOCUS       DAOMPD010000453         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19261_length_2223_cov_2.318727, whole genome shotgun sequence.
ACCESSION   DAOMPD010000453 DAOMPD010000000
VERSION     DAOMPD010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19261_length_2223_cov_2.318727"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLY93_12730"
     CDS             complement(<1..703)
                     /locus_tag="PLY93_12730"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70391.1"
                     /translation="MHIAIIDVGSNNIKLEVFELSTDGHSHLIFNTKFPARLGSDVFV
                     THRLRAENVEQAIAAFQKIHRITLDYACEATVAIATAALRECENSDFIERAKREAQIS
                     ISVISGLEEARLVYNGVRAHTSFGEKNYFINDIGGGSTEILVANDNAIHFAQSLRLGT
                     VRLKEMFGSDSKETFKLLERYVARSLEPYLADIKEKRIEAALCTGGNSRALLDVLREM
                     NAPIKEELHLPLIETK"
     gene            complement(710..1693)
                     /locus_tag="PLY93_12735"
     CDS             complement(710..1693)
                     /locus_tag="PLY93_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRP70392.1"
                     /translation="MEGSGRVTQVAAEKKQAPKGTPPGPIVYIFLDGVGIGNSNADQN
                     PFARFETQFFSAYAGGKFEKPGQLLVTDAHMGIPGLPQSATGQTALFTGYNGPKILGR
                     HVNGFPTFTLRPYFKEKNILKLLNDAGYNATLLNSYSPLYLERLNHPRAERILSASSL
                     MQLNSGKPYFTTDDYFSGRSLYMDMTNWFLRKHGTQVPLVDAYENGRKLVQIARNYDL
                     VIFEYFFTDKVGHAQSWGAAKRIIHHVDNFLKGVWEELDAQKETVVISSDHGNIEDLS
                     IRTHTKNPVGTFIYGKEERAFATQVHSLLDIPRVILSLFHLPFTEDYQPSL"
     gene            complement(1696..>2223)
                     /locus_tag="PLY93_12740"
     CDS             complement(1696..>2223)
                     /locus_tag="PLY93_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRP70393.1"
                     /translation="RNYWLDQIVVLMGGRLAEEIQFQDVTTGASNDIERATQIARHMV
                     TEWGMSEKIGPIRLSSSDSGSVFLGRDYARKGDHSEEYATIVDSEVKRIIDTAFTRGR
                     MLLKKNKKRLDQVASALLERETITGSELREIMSGRKLKPLPPAPEPTAKNDDKKPTGQ
                     TPAFGTDLLSGAQPA"
BASE COUNT          517 a          517 c          575 g          614 t
ORIGIN      
        1 ctttagtttc aatgagcggc aagtggagtt cttctttgat gggagcgttc atttcccgca
       61 acacatcgag caatgcgcgt gagttgccgc cggtgcatag cgccgcctcg atgcgttttt
      121 ccttgatgtc ggcgaggtag ggctctaaag agcgggcaac gtaccgctca agcagtttaa
      181 aagtctcttt tgaatcgctg ccaaacatct ctttgaggcg cacggtgcct aaacgtaaac
      241 tttgagcaaa gtgtatcgcg ttgtcgttcg ccacgagaat ttccgtagaa ccgccgccaa
      301 tatcgttgat gaaataattt ttctctccaa acgaagtgtg cgcgcgaaca ccgttataga
      361 caagccgcgc ttcttctaat cctgaaataa ctgaaataga aatttgcgct tcacgttttg
      421 cacgctcgat aaagtcagag ttttcgcatt cgcgtagcgc cgccgtggca attgccaccg
      481 ttgcttcgca ggcataatcc aaagtgatgc ggtgtatttt ttggaaggcg gcgatcgctt
      541 gttcgacgtt ttcggcacgc agccggtggg tgacaaaaac gtcgctccct aaccgtgcgg
      601 gaaattttgt attaaaaata agatgcgaat ggccgtcggt gcttaattca aaaacttcaa
      661 gtttgatgtt gttcgaaccg acgtctataa ttgcgatatg cactttggat tacaaactcg
      721 gttgataatc ttccgtaaac ggtaaatgaa aaagcgataa aataacgcgc ggtatatcta
      781 acagagaatg cacttgtgtt gcaaatgccc tctcttcttt gccgtaaata aaagtcccga
      841 cgggattttt tgtatgtgta cgaatcgata aatcttcgat gttgccgtgg tcggagctaa
      901 tcacgacggt ttctttttga gcgtcgagtt cttcccagac tccttttaga aaattgtcga
      961 cgtggtggat aatccgcttg gccgctcccc acgattgcgc gtggccgact ttgtcggtaa
     1021 aaaaatattc aaaaatgact aagtcgtagt tacgcgcaat ttgaaccagt tttcggccat
     1081 tctcgtaagc gtcgacaagc ggcacttggg tgccgtgctt gcgcaaaaac caattcgtca
     1141 tgtccatgta aagactgcgg cctgaaaagt aatcgtcggt ggtgaagtag ggcttacccg
     1201 agttgagctg catgagcgac gatgccgaaa gtatacgttc ggcgcgggga tggttcaagc
     1261 gttccaggta taacggtgaa tacgaattga gtagcgttgc gttgtagccc gcatcgttga
     1321 ggagttttaa gatatttttt tctttgaaat aaggccgcag tgtgaatgta ggaaatccat
     1381 tcacgtgccg ccctaaaatt ttggggccat tgtaaccggt aaaaagcgca gtttgtcctg
     1441 tcgccgattg ggggagccct gggattccca tgtgtgcgtc ggttacaagc aattggcctg
     1501 gcttttcaaa tttgccacca gcgtatgcgc taaaaaattg agtctcgaaa cgcgcgaatg
     1561 ggttttgatc ggcgtttgag ttaccgatac ccacaccgtc caaaaaaata tagacgatgg
     1621 ggccaggggg agttccttta ggcgcttgtt ttttctctgc cgctacctga gtgactctcc
     1681 ccgagccttc catagctacg cgggctgtgc ccctgaaagt aagtcggtgc caaaagcggg
     1741 cgtttgtcct gtcggctttt tatcatcgtt tttggccgtc ggttcgggcg ctggggggag
     1801 tggctttaat ttacggccac tcatgatttc acgtaactcg ctgccggtga tggtttcacg
     1861 ttccaaaagg gcagatgcga cttgatcgag gcgtttttta tttttcttta agagcatgcg
     1921 tccgcgcgta aacgccgtgt cgatgatacg tttcacttcg ctatctacaa tcgttgcata
     1981 ctcttcggag tgatcgccct tgcgcgcata gtcacgccct aaaaagacag aaccggagtc
     2041 cgaactcgac aagcggatgg gaccaatttt ttcagacatg ccccattcgg tcaccatgtg
     2101 ccttgcaatt tgtgtcgcgc gttcgatgtc gttagaggcg ccggtagtca cgtcttggaa
     2161 ttggatttct tcggctaagc gaccgcccat gagcaccaca atttggtcga gccaatagtt
     2221 gcg
//
LOCUS       DAOMPD010000454         2221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19284_length_2221_cov_2.289474, whole genome shotgun sequence.
ACCESSION   DAOMPD010000454 DAOMPD010000000
VERSION     DAOMPD010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19284_length_2221_cov_2.289474"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PLY93_12745"
     CDS             <1..1821
                     /locus_tag="PLY93_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRP70394.1"
                     /translation="KVIFGDHVTVDAGTGIVHTAPGHGQDDYNVGLKYGLDVLSPVDN
                     RGRYTSEFAELEGTFVFDANPLIIEKMRSNGTLLFATTFVHQYPHSWRSHKPLIMRAT
                     PQWFMKIEPLREKALEVIKETQWIPEWGESRFTAAVKSRPDWCLSRQRYWGVPIPAFQ
                     CKNCGHVHLDEASLNHVIGFVEKEGVEVWYEKPALELLPQGTKCSDCGGADFDKENDI
                     LDVWFDSGVSWYAVLKQNPDLGFPADVYLEGSDQHRGWFQSSLWPSLALTGVAPFKKV
                     VTHGYILDAQGRAMSKSLGNGMSPRTDIIDKFGADILRLWVASCDYRTDNRIGKEMIN
                     HLTDAYRKIRNTLRYLLGNTQDGRIVERPALSVAEGSRNDTAITEKLDLWVLAEAHEL
                     VTQVTDAYEESEFHLVFQKILQFCAVTLSNLYLDMVRDTLYCDGDPRSDKNTPASEIY
                     HRRRESTLTTLKILTDTLVTLLAPVLSFTAEETHRTYNQSQSVFESPWPDLSRFENTN
                     LLKEFSELKTLRDEVNTAIEPLRKAGEIGSNVEVEVEIPQKGDENTLLRFLGVSALKT
                     DALTLKVRKSEKPKCPRCWLHRDLKESGLCERCDTVTNSI"
     gene            1853..2086
                     /locus_tag="PLY93_12750"
                     /pseudo
     CDS             1853..2086
                     /locus_tag="PLY93_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
BASE COUNT          576 a          524 c          588 g          533 t
ORIGIN      
        1 aaagtgattt ttggcgatca cgttactgtc gatgcgggta ccggcattgt gcatacagcg
       61 ccaggccacg ggcaagacga ctacaatgtg gggttgaagt acggcctcga tgttttgtct
      121 ccggtggata accgtgggcg ttacacgagt gaatttgctg aattagaagg cacttttgtt
      181 tttgatgcca atcccctaat tattgaaaaa atgagatcga atggaacgct gctctttgcg
      241 accacttttg tacaccagta tccacattcg tggcgctcgc acaagccgct catcatgcgg
      301 gccacaccgc agtggtttat gaaaattgaa ccgctgcgtg aaaaggcgct cgaagttatt
      361 aaggaaaccc aatggattcc cgaatggggc gagagccgtt ttactgcagc ggtcaaaagc
      421 cgccctgatt ggtgtctatc ccgccaacgc tattggggtg taccgatacc cgcgttccag
      481 tgcaaaaatt gcggccacgt gcatttggat gaagcctcgc tcaaccacgt tatcggtttt
      541 gtcgaaaaag aaggcgtcga agtgtggtat gaaaaaccag cgttagaact tttgccccag
      601 ggcacgaaat gcagtgattg cggcggcgcc gatttcgata aagaaaacga tattttagac
      661 gtgtggtttg acagtggagt gagttggtac gcggtcttga aacaaaaccc tgatctaggc
      721 tttcccgccg atgtgtattt ggaaggttct gaccaacacc gcggttggtt tcaatcgtcg
      781 ctgtggccgt ctttggcgct taccggtgtt gctcctttca agaaagtggt gacgcatggt
      841 tatattttag acgcccaagg ccgagcgatg tccaagagtc tcggcaacgg catgagccca
      901 cgcaccgaca tcatcgataa attcggcgct gacattttgc ggctgtgggt ggcgagctgc
      961 gactaccgca ccgacaaccg catcggaaaa gaaatgatca atcacttgac cgacgcctac
     1021 cgtaaaattc gcaacacgct gcggtatctt ttaggtaata cgcaggatgg gcgtatcgtt
     1081 gagcgtcctg ccctgagcgt agccgaaggg agccgaaacg atacggccat taccgaaaag
     1141 ctcgatttat gggtacttgc tgaggcgcac gagttggtaa cgcaggttac cgacgcgtat
     1201 gaagagtcgg agtttcactt ggtctttcaa aaaattctac aattttgtgc tgtcactctc
     1261 tccaatttat atctcgacat ggtgcgcgat acgctctact gcgatgggga tcctcgttct
     1321 gacaaaaata ctcctgccag cgagatttat catcggcgca gagagtcgac gttaacgacg
     1381 ctcaaaattt taaccgacac ccttgtgacg ctcttagcgc cggtgttgag ctttaccgcc
     1441 gaagaaaccc accgcacgta taaccaaagc caaagtgttt ttgagagccc atggccggat
     1501 ttatcacggt ttgaaaatac aaatttactc aaagagttta gtgaactaaa gacactgcgc
     1561 gacgaggtga acaccgcgat tgagccttta cgcaaagcag gtgaaattgg ctcaaacgtc
     1621 gaggttgagg tagagatacc ccaaaaaggc gatgaaaata ctttgctgcg ctttttggga
     1681 gtttctgctt tgaaaacaga tgcattgaca ctcaaagtga ggaaatccga gaaaccaaaa
     1741 tgcccgcgct gctggttaca ccgcgattta aaagagtcgg ggctgtgcga acggtgtgac
     1801 acagtgacca attccatttg acgcggtgtt tttaagtccg gtttagcttt gtatgcccac
     1861 gtacgattac aaatgtgccg aatgtggcaa tgtgtttgaa aaattccacg gcatgaacga
     1921 aacgccagcg gttgagtgcc caagctgtgc atctaaaaac acagcgcggc aggtgagctt
     1981 gggtgcaggg attgttttta agggggcggg gttttatgtg aacgactaca aaaaagacgc
     2041 cggtgcgtca aagagtccta cagccccggc ggcaaaaaat actgaggcat cggtcaagac
     2101 cgaaagcgct cccactaaaa cagagaacgc cgcacctgct tcatcgcatt catgcgggcc
     2161 cagttgttcg cacacatagc tgtgcttatg aactcactgc gcatcgagcg agtattagta
     2221 t
//
LOCUS       DAOMPD010000455         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19375_length_2212_cov_1.628651, whole genome shotgun sequence.
ACCESSION   DAOMPD010000455 DAOMPD010000000
VERSION     DAOMPD010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19375_length_2212_cov_1.628651"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>760
                     /locus_tag="PLY93_12755"
     CDS             <1..>760
                     /locus_tag="PLY93_12755"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP70395.1"
                     /translation="FLIAILLIACRGEPYHSFGDAWRTLPAEMQGEVQGLPAHWASVG
                     TEGAVSTANALASEAAIQMLQAGGNAIDALLAAQWVLAVTEPQSSGLGGGGFLLYYQA
                     KSKRVYALDGREEAPSGIDETIFLTKENKPMPFLMRVEGARAVGVPGTVALMEYAKKR
                     FGNSQIPFARTFESALKMARQGIRVSSRLSQAMHLNRARFLKQNRGYNPYLHSDKPYN
                     VGEILYQDDLADTLALLKEEGTASFYKGAIAKDIL"
     assembly_gap    761..823
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <824..1756
                     /locus_tag="PLY93_12760"
     CDS             <824..1756
                     /locus_tag="PLY93_12760"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRP70396.1"
                     /translation="PVAASTMTDSDLKNYRVAERPVAVRCIGKNIFYSMSAPASGAYT
                     LAAVTNAPQSASEDSFFKASLAAQQQSFIQREKTLEDPDWNWVKKNQSVPEKSSAHES
                     ENTTHVSIVDVEGNIVSYTASVEMSMGSALFVQGRGFILNNELTDFTPIPKRVNAVEQ
                     GKRGRVTALNAEARETVGGKRPNSSMSPLIFEREDGTFFALGSPGGPTIVGVNALVAA
                     RLLRREELAAAVLAPRAIAMPSGHIIAELVIKQNRELVEALHRSGFPIDTTRKIISLG
                     SVQAVSFNPKTNTYRAVSDLRREGLALVAKPKVD"
     gene            1758..>2212
                     /locus_tag="PLY93_12765"
     CDS             1758..>2212
                     /locus_tag="PLY93_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HRP70397.1"
                     /translation="MQLLDIVVIASYFILTFGVGILATRKKNETTEDYFLSGRKLPWH
                     LAGLSMVATTFAADTPLAVTGITLERGIAGNFLWLSLIPAGLMTAIFYARLWRRSGVV
                     TDAEFATLRYTGRSAMFLRRFRALYLALPVNLIIMGWVTTGMAKVMAVF"
BASE COUNT          492 a          531 c          594 g          532 t
ORIGIN      
        1 atttccttat tgcaattttg ctcatcgcct gccgcggtga accctaccat agctttgggg
       61 atgcgtggcg cacgctgcct gcggaaatgc aaggcgaagt gcaaggcttg cccgcgcatt
      121 gggcgagcgt cggcacagaa ggggcggttt cgaccgcgaa tgcacttgca tccgaagcgg
      181 caatccaaat gctacaagca ggtggaaacg cgatcgatgc gcttctcgca gcgcagtggg
      241 ttttggcggt caccgagccg caatcttccg gtttaggagg tgggggattt cttctttatt
      301 atcaagcgaa gtcgaagcgg gtgtatgcgc tcgatggccg cgaagaagct ccctctggaa
      361 tcgatgaaac aattttttta acaaaagaaa ataaaccgat gccgttttta atgcgcgtgg
      421 aaggcgcgcg tgcggtgggc gtccccggga ccgtcgcgct catggagtat gccaaaaaac
      481 gctttggcaa ttcacaaatt ccgtttgcca gaacgtttga aagcgcgctg aagatggcga
      541 ggcaaggcat ccgcgtttcg tcacgcctct cgcaggcaat gcatctcaac cgcgctcgat
      601 ttttgaaaca gaacaggggc tataatcctt atctccattc ggataaacct tataacgtcg
      661 gtgaaatttt ataccaagac gatttagcag acacactcgc gcttcttaag gaagagggca
      721 cggcatcttt ttacaaaggc gcgattgcaa aagacattct nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctgtcg ccgcttcaac
      841 aatgacagat agcgatttga aaaattatcg tgtggccgag cgtcctgtgg cggtgcgttg
      901 tattggaaaa aatatttttt actcgatgag tgcgcctgct tcgggggcgt acacattagc
      961 ggcggtaacc aatgcgcctc aatcggccag tgaagactct ttttttaaag cgtctttagc
     1021 ggcgcagcag caaagtttta tccagagaga aaaaacgctc gaagaccccg attggaattg
     1081 ggtgaaaaaa aatcaaagtg tacctgaaaa atccagtgcg catgaaagtg aaaacaccac
     1141 gcacgtttcc attgtcgacg tggaaggaaa tatcgtgagc tacaccgcgt cggtggagat
     1201 gagtatgggg agtgcgctct ttgttcaagg gcggggtttt atcttaaata acgaactcac
     1261 cgactttacg cccattccca agcgcgtgaa cgcagtcgag cagggcaaga gaggccgcgt
     1321 aacagcgctc aatgcagagg cgcgtgaaac ggtggggggg aagcgtccca acagctcgat
     1381 gtctcccctt atttttgaac gagaagatgg cacttttttt gcattgggtt cgccgggagg
     1441 gccaacgatt gtcggcgtca acgcgcttgt tgccgctcgc cttctgcgcc gcgaagaatt
     1501 ggcagcggcg gttttagcgc cacgcgccat cgcgatgccg agtggtcata taattgccga
     1561 gcttgtcatt aagcaaaatc gagaactcgt cgaggcgctc caccgcagtg gctttcccat
     1621 cgacactacg cgcaaaatta tttcattggg ttcagtacaa gcagtgtctt tcaaccctaa
     1681 aactaacacg taccgcgcgg tgagtgattt acgccgtgag ggcttggcgc tcgttgccaa
     1741 acctaaggtc gattaacgtg caactcctcg acatcgtcgt catcgcttcg tattttattc
     1801 ttacttttgg tgtgggtatt ttagccaccc gtaaaaagaa cgaaaccacc gaagattatt
     1861 ttttatcggg gcggaaactc ccgtggcatt tggcggggct ttcgatggtc gccactacat
     1921 ttgctgccga caccccgttg gcggttaccg gtatcactct tgagcgtggt atcgcaggga
     1981 attttttgtg gttgtcgctc atccccgctg gcctaatgac cgctattttt tatgcgcggt
     2041 tgtggcggcg ttcgggggtc gtcaccgacg ccgagttcgc cacgctgcgt tacaccggac
     2101 gctctgcgat gtttttacgc cgcttccgtg cgctctactt ggctttaccg gttaacctta
     2161 tcatcatggg ttgggtgacg acggggatgg caaaagtgat ggcggttttt tt
//
LOCUS       DAOMPD010000456         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19435_length_2207_cov_1.947026, whole genome shotgun sequence.
ACCESSION   DAOMPD010000456 DAOMPD010000000
VERSION     DAOMPD010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19435_length_2207_cov_1.947026"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..920
                     /locus_tag="PLY93_12770"
     CDS             108..920
                     /locus_tag="PLY93_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70398.1"
                     /translation="MMQDIPYDQPFSEFLIYLRKAYPKAQYGEAFIPIIAHKATAGYV
                     IQKHFPELMPEFMRLNGQFAYAISEPNWGNSPLNIKSTLKENTDGTFELYANKSFVLD
                     GDIGIFVAQKITPLNDENPAKEAKTFALVIVDTDCYRTTRTALGTPEATYRVDENTYM
                     THYRCEFTVTLHNALFTPISKREILMLARTIVFREQVAYAVLAYLEAAKYADMKATLS
                     RVELLCQRAKEEPGQDDIAVAREILSASVIRILDRTDAHPFWKWLSRFIKPT"
     gene            complement(907..>1344)
                     /locus_tag="PLY93_12775"
     CDS             complement(907..>1344)
                     /locus_tag="PLY93_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70399.1"
                     /translation="LLFIAEMAERLSTKSYMGEDEAKSLLEKFDALPEILTWGDYFAA
                     EIATEHWQKSDAEFENICETVVFDFIAAIYIFTDKTSAFLVCARDEYRNALLQTEESV
                     ERDEKIHLGILEEYFTQMGLSKEKLNSEDTEFFDAFNVKAKSA"
     assembly_gap    1345..1364
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1489..>2207
                     /locus_tag="PLY93_12780"
     CDS             1489..>2207
                     /locus_tag="PLY93_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRP70400.1"
                     /translation="MARQAVRVTAPRKTKKRTRLAEIGPEIEIKNEEPVVDRSLAKNP
                     YTESSIQWYLEEINKIPLLTREEEDKLARATVAGDAKAKERLITSNLRFVVQVAKKYQ
                     KFGISLLDLINEGNLGLIKAAERFDPDMGYHFISYAVWWIRQAIILAINQKAAMIRLP
                     MNRTIDVARIESIQKDLENELGEEPTLAQIADRLDMPEAEVRWLKQISSDYVSLDVPA
                     NTDSENTQILQVRDEKYISPE"
BASE COUNT          609 a          546 c          482 g          550 t
ORIGIN      
        1 cgagtggagg gcttgtacgg taaaattatc tcttgctgct atcacggtat atacggacaa
       61 tttttttcat cgacttgtcc agtcgtctca aaagcacaac ggtgtaaatg atgcaagata
      121 taccctatga tcaacccttc tctgagtttc tcatctattt gcgcaaagcg taccccaaag
      181 cgcaatacgg cgaggcgttt ataccgataa tcgctcacaa agccaccgcg ggctacgtta
      241 tccaaaaaca ttttcccgaa cttatgcccg agttcatgcg tctcaacggt caattcgctt
      301 atgcgatctc tgaacccaat tggggaaact cacccttaaa catcaaaagt accctcaaag
      361 aaaacaccga cggcacattt gaactctacg ccaataaatc atttgtgtta gacggcgaca
      421 tcggtatttt tgtcgcacaa aaaataacgc cgctcaacga cgaaaatccc gctaaagagg
      481 caaaaacttt tgcgctcgtg attgtcgata cggattgtta ccgcactaca cgcaccgcac
      541 tcggcacccc cgaggcgacg taccgcgtgg atgaaaatac gtacatgaca cattaccgct
      601 gtgaatttac ggtgactctc cacaacgcgc tttttactcc gatctctaag cgagaaatcc
      661 tgatgcttgc gcgcacgatt gtttttcgtg agcaagtcgc atacgcggtg ctggcatatc
      721 ttgaagcggc gaaatacgca gacatgaaag caacgctttc tcgcgtcgag ttactctgtc
      781 agcgcgcgaa agaagaacca gggcaagacg acatcgccgt tgcgcgtgaa attttgtcgg
      841 cgtcggtgat tcggattctc gaccgcaccg atgcacatcc tttttggaaa tggctttcgc
      901 gttttattaa gccgacttag ctttaacgtt aaaggcgtcg aagaattccg tatcctcgct
      961 attgagtttt tctttggaga gtcccatttg tgtaaaatac tcttctaaaa tgcccaagtg
     1021 tattttttca tcacgctcga cactttcttc tgtttgcagg agtgcattac ggtattcgtc
     1081 gcgcgcgcag acaaggaatg cagacgtctt gtcggtaaag atataaatgg cggcgatgaa
     1141 atcgaacacg acagtttcac aaatattctc aaattcggca tcgctttttt gccagtgttc
     1201 ggtggcgatt tcagcggcga aataatcgcc ccatgttaat atctcaggca atgcgtcaaa
     1261 tttctcgaga agacttttag cctcatcctc gcccatgtac gattttgtcg aaagccgctc
     1321 cgccatctcg gcaataaata gcaannnnnn nnnnnnnnnn nnnngggggg atagtttttt
     1381 ttgtagcaca aagctttcga gtgcggggcg attttgcata gttagttcat tatcggaaaa
     1441 agtccgtatc cgctttacgc gctgttttat ataccgttat cggtctaaat ggcgaggcag
     1501 gctgtgagag taaccgcacc tagaaaaacg aagaaacgca cccgcttagc agaaatcggg
     1561 cctgaaattg aaattaaaaa cgaagaacca gtcgtcgata gatctctcgc aaaaaatccc
     1621 tataccgaat caagcatcca atggtatctt gaagagatca ataagattcc acttttaacg
     1681 cgtgaagaag aagataagct cgcacgcgca accgtggctg gtgacgctaa agcgaaagaa
     1741 cggctcatta cctccaacct acgctttgtc gtgcaagtcg ccaagaagta tcaaaaattt
     1801 ggtatctcgc ttttggatct catcaacgaa ggtaacttag gactcattaa agccgccgaa
     1861 cgcttcgacc ctgacatggg gtatcatttc attagctacg ccgtttggtg gatccgccaa
     1921 gcgattattt tagcgatcaa tcaaaaagcg gcgatgatcc gcctgccgat gaaccgcacc
     1981 atcgacgtgg cgcgtattga gtcgatccaa aaagatttag agaacgaatt gggcgaagaa
     2041 cccacactcg ctcaaattgc cgaccgcctc gacatgcccg aggcagaggt acgctggctt
     2101 aaacaaatct cctccgatta cgtatcgctc gacgtgcccg ccaacaccga ttcagaaaac
     2161 acccaaattt tgcaagtgcg cgacgaaaaa tatatttctc ctgaaaa
//
LOCUS       DAOMPD010000457         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19465_length_2205_cov_2.428837, whole genome shotgun sequence.
ACCESSION   DAOMPD010000457 DAOMPD010000000
VERSION     DAOMPD010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19465_length_2205_cov_2.428837"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..539)
                     /locus_tag="PLY93_12785"
     CDS             complement(51..539)
                     /locus_tag="PLY93_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70401.1"
                     /translation="MRSIHHLFLLLGGLLAFMPHCTAGVAHSVQVILPTPHVNVQTGA
                     ARQIGNLFFYTDRNIPETSNAYNALLQGKTAETLDILSQIEGSNLPDIQQAYWQNDVA
                     VCFILDGRYKEADELLMQASLLADEEAIMHNKRIAVYLHESWRTLKERKKVEVAPPSK
                     QP"
     gene            624..1565
                     /gene="trxB"
                     /locus_tag="PLY93_12790"
     CDS             624..1565
                     /gene="trxB"
                     /locus_tag="PLY93_12790"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRP70402.1"
                     /translation="MNENHYNTIIIGSGPAGYTAAIYAGRANLKVLVLEGIERGGQLM
                     ITSEVENYPGFPEGIQGPELMEHFRNQALRFGATLKSVLADKAELKDGAPFKVTAEGT
                     TYTGDTVIIATGASARFLGISSEEKYKGKGVSACATCDGFFFRGKEVAVVGGGDSAFE
                     EATFLTKFASKVTLLHRRDTFRASKIMVERAEKNPKISIKPFVEVSEVYGEEKTGTVT
                     GLKLKNTQSGVVEDFPVQGLFIAIGHDPNTALFKGQLDMDATGYLKTQPGRTATNIPG
                     VFAAGDVQDHYYRQAITAAGSGCQAAIEAERYLESKG"
     gene            1574..>2205
                     /locus_tag="PLY93_12795"
     CDS             1574..>2205
                     /locus_tag="PLY93_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRP70403.1"
                     /translation="MQDFESRPAYFEGKFVPLKDANVNIRTHALQYATAFFGGMRGYY
                     NASKKNVYVFRLEDHHRRLRQSAKILRFHWAMPFEEFREITVKLLRDGNWRENCYLRP
                     FIYKSELSMTPRLHNVEDRFSLYAIPLNDYLDTTRGMQVQVSSWMRIADNQIPARAKA
                     TGGYINSALAKSEALENGFDEAIFLDQRGFVCEGSAENIFLVRGNTLITPG"
BASE COUNT          558 a          537 c          566 g          544 t
ORIGIN      
        1 ggtattcacc tcaaacaaga ctggtttcct ggatacactg acgctacgta ttaaggctgt
       61 tttgaaggcg gagcaacttc caccttcttg cgctctttaa gcgtgcgcca cgattcgtgg
      121 aggtacaccg cgatacgctt gttgtgcata atcgcttctt cgtcggcaag aaggctcgct
      181 tgcatcaaga gttcatccgc ctctttatag cggccgtcga gaataaaaca caccgccaca
      241 tcgttttgcc aatacgcttg ttggatgtcg ggtaaattgc tcccttcaat ctgtgatagt
      301 atatcgagtg tctcggcggt ttttccttgc aagagcgcgt tgtacgcatt gcttgtttcc
      361 ggaatgttgc ggtcggtgta aaaaaataaa ttcccaattt gacgcgcggc gccggtttgt
      421 acattcacat gcggtgtggg tagaatgact tgcacgctgt gcgccacacc cgcagtgcag
      481 tggggcatga aagcgagtaa tccccctaaa agtaaaaaaa ggtggtgtat ggagcgcatg
      541 tgcccatttc gtttttcgtg cgcgggcaga aaacataaaa tgaaaattgt ccgtgtgagt
      601 cgatttagag tatcttctca actatgaatg aaaatcatta caatacaatt attattggct
      661 cgggccctgc gggctatacc gcagccattt atgcagggcg tgcaaacctt aaagtcctcg
      721 tcttagaagg gattgagcgc ggcggtcagc tcatgatcac gagcgaagtg gaaaattatc
      781 cgggctttcc cgaaggtata caaggccccg aactcatgga gcatttccgt aaccaagcgt
      841 tgcgttttgg agcaacatta aaatccgtat tggccgacaa agcagaactt aaagacggtg
      901 caccgttcaa agtgaccgcc gagggtacaa cgtacaccgg cgacacggtg attatcgcca
      961 ccggtgcatc ggcgcgcttt ttgggaatat cttccgagga aaaatataaa ggcaagggcg
     1021 tgtcggcgtg tgcgacgtgc gacggttttt tcttccgtgg taaagaagtc gcggtggtcg
     1081 gcggtggcga ctcggcgttt gaagaagcga ccttcctcac taaatttgca agtaaggtga
     1141 cgctgctgca ccgccgtgac actttccgtg cgagtaaaat tatggtcgag cgcgcggaaa
     1201 aaaatcctaa aattagcatc aaaccttttg tcgaagtctc tgaagtctat ggcgaagaaa
     1261 aaaccggtac cgtcaccgga ttaaaactga aaaatacgca atcgggcgtg gtggaagatt
     1321 ttccggtaca agggctattc attgcgattg gacacgatcc caacactgcg cttttcaaag
     1381 gccaactgga catggatgcc accggttatt tgaaaaccca acccggacgc accgcgacga
     1441 acatccctgg tgtttttgcc gccggtgacg tgcaagacca ttattaccgc caagcgatta
     1501 ccgcagcggg gtcgggctgc caagcggcga ttgaagccga gcgctattta gaatcaaaag
     1561 gttagtcaac gccatgcaag atttcgaatc gcgccccgcg tactttgaag ggaaatttgt
     1621 tccgctcaaa gacgcgaatg tgaatatccg cacccacgcg ctgcaatacg ccactgcatt
     1681 tttcggtggc atgcgcggct attacaacgc gtctaaaaag aacgtgtacg tattccgcct
     1741 cgaagaccac caccgccggc tcaggcaatc cgccaaaatt ttacgcttcc attgggcgat
     1801 gccctttgaa gagtttcgtg agattacagt aaaacttttg cgcgatggca actggcgtga
     1861 gaactgctat ctgcgtccgt ttatctataa atccgaattg tcgatgacgc cgcgcctgca
     1921 caatgtggaa gatcgctttt ctctctatgc aatccctttg aacgattatt tagacaccac
     1981 gcgcggtatg caagtgcagg tttcgtcgtg gatgcgtatt gcggacaacc aaatccccgc
     2041 acgcgcaaaa gccacgggtg gttacatcaa ttctgcactt gcaaaaagcg aagctctgga
     2101 aaatggtttt gacgaagcaa tttttctcga ccaacgcggt tttgtgtgcg aaggatctgc
     2161 cgaaaatata tttttggtgc gtggaaatac gctcatcact cctgg
//
LOCUS       DAOMPD010000458         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19485_length_2203_cov_2.906425, whole genome shotgun sequence.
ACCESSION   DAOMPD010000458 DAOMPD010000000
VERSION     DAOMPD010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19485_length_2203_cov_2.906425"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLY93_12800"
     CDS             complement(<1..291)
                     /locus_tag="PLY93_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70404.1"
                     /translation="MINKIPQFIFTACIFLSIPYLLHAENNPPEIQEVTIELRTLSVS
                     MAQKNLIHFLEKNDGYFVYQNPRTVAGYVPLNVPLETIFAAAAKEGHVRSRNI"
     gene            complement(284..1450)
                     /locus_tag="PLY93_12805"
     CDS             complement(284..1450)
                     /locus_tag="PLY93_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRP70405.1"
                     /translation="MNFMVAMRRCTVLIFLSPILFFSFNCRSMATQSEADGYGGSSKG
                     TTHSAVPSPTSEAAPESPKPMAKKSVAKKTARMVVYVGQIAVEVFELKEKTTALEALA
                     KNLGGFIESNKTTEDYARIVLRVPVKRFNEALESISNEGRVLSREVSAADITKEFSDL
                     ETRLKNNIAVRERLYTLLKSAKKVKARLKILQEINRITQLITADEERLKNMASAASYS
                     TVIATLQLFKSPTTIRMPSPFSWIASLVPERRSILTYKDIELPLPASFFDYEKEFKKE
                     KVEALFISARQSKIRAGRVKNDPKADAVFWRQALEKEMLSRGYVKKSRDDVDTGSIQL
                     YSYVDGLEEHFYLIAFFTREKYLYVIEAYYPNKQELDELDKNVRTSIKEVKIHD"
     gene            1603..>2203
                     /locus_tag="PLY93_12810"
     CDS             1603..>2203
                     /locus_tag="PLY93_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HRP70406.1"
                     /translation="MEPLKELTQTVTELPRGGYLAQTPGGYFQFGAPPETIKDTMFLP
                     EGVPTVFVLPEHFFNWRKGISVAELEFPIYYNFFLKKRKTILICNREQFLRFKTVFNE
                     SVFGPKELDVAQDYMEGLEVPQLKREMKFFSSTFKFSDMVGFGLFNEHNRFTYNGVTV
                     ARTAEGNFSLSKGDAHIANVPGKIEFKTIYRIGTRLHEPF"
BASE COUNT          555 a          497 c          517 g          634 t
ORIGIN      
        1 tatattgcgc gagcgtacat gaccttcttt cgcagcggcg gcaaaaattg tttccagcgg
       61 aacattcagt ggtacatatc ccgctactgt gcgcgggttt tggtacacga agtaaccgtc
      121 gtttttttcc agaaaatgga ttaagttttt ttgagccatt gagacgctga gcgtgcgcag
      181 ctcgatcgtc acctcttgaa tttcaggggg attattttct gcgtggagga gataaggtat
      241 gcttaaaaaa atgcacgcgg taaatataaa ttgtggaatt ttgttaatca tggattttta
      301 cctctttgat gctggtacgc acatttttat cgagttcgtc caactcttgc ttgttgggat
      361 agtacgcttc gatgacgtac aaatattttt cgcgggtgaa aaacgctatt aaataaaaat
      421 gctcttccaa tccgtcgaca taactgtata gttgtatcga acccgtgtcc acatcgtcgc
      481 gcgatttttt tacatagccg cgcgagagca tttctttttc taacgcttgc cgccaaaaaa
      541 cggcgtcggc tttggggtcg ttcttcacgc gacccgcacg gattttcgat tggcgcgccg
      601 aaataaataa cgcctctact ttttcttttt tgaactcttt ctcatagtca aaaaaagagg
      661 caggcaaggg cagttcgatg tctttatacg tcagaatgct gcgcctttca ggaaccagag
      721 aggcaatcca actgaaagga gacggcatgc ggatggtggt gggacttttg aaaagttgca
      781 gagtggcaat gaccgtcgaa tacgacgctg cgcttgccat gtttttgaga cgctcttcgt
      841 cggcggtgat gagttgggtt atacggttga tctcttgtag aattttgagg cgcgctttta
      901 ctttcttggc gcttttgaga agagtataaa gtcgctcgcg cacggcaatg ttgtttttca
      961 ggcgtgtctc taaatcgcta aattctttgg taatgtcggc agcgcttact tcgcggctta
     1021 gcacgcgccc ttcgttagag atgctctcca acgcttcgtt aaaacgcttc accggcacgc
     1081 gcaagacgat gcgcgcgtaa tcttcggtgg ttttattcga ctcaataaac cctcccaaat
     1141 ttttggcgag tgcttcgagt gcggttgttt tttctttgag ttcaaaaacc tccactgcaa
     1201 tttgccctac ataaaccacc atgcgcgctg tttttttagc gacacttttt ttagccatgg
     1261 gttttggact ttctggggca gcttcggacg tagggctggg gactgcgcta tgagtggtgc
     1321 ccttagagct tcccccgtat ccatcggcct cactctgcgt tgccatgctg cggcagttga
     1381 atgagaaaaa tagaataggg cttagaaaaa ttaaaactgt gcatcgccgc atagccacca
     1441 taaagttcat atagctaaca ttgctgtaca gacacgacgt gcaaatcgaa aaaattgtct
     1501 cagcgagttg gcgcacaggc tcgtagatat tgagaaaatt ctttacaata ccattaaaca
     1561 gtcgtaaaga gttgaaacag atcgtcaaaa agactttata acgtggaacc tcttaaagaa
     1621 cttactcaaa ccgtcactga actaccgcgt ggcggctatt tagcgcaaac tcctggcggc
     1681 tattttcaat tcggtgcacc acccgaaacg atcaaagaca cgatgttttt gcccgaaggt
     1741 gtgccgacgg tttttgtact accagagcac tttttcaact ggcgcaaagg cattagcgtc
     1801 gccgagttgg aattccccat ctactataat ttctttctca aaaaaagaaa aacgattcta
     1861 atttgtaacc gcgaacaatt tttgcgtttc aaaaccgttt tcaacgaatc ggtgttcggt
     1921 cccaaagaac tcgatgtggc gcaagattac atggaaggtt tggaagtgcc gcagcttaaa
     1981 cgcgagatga aatttttcag tagcacgttt aagttttccg acatggtcgg ctttggtctt
     2041 ttcaacgagc ataaccgttt cacttataac ggcgtcacag tggcgcgcac cgccgagggt
     2101 aatttttcgc tctccaaagg agacgcacac attgccaacg tgccaggaaa aattgagttt
     2161 aaaacgattt accgaatcgg cacgcgcttg cacgagcctt ttg
//
LOCUS       DAOMPD010000459         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19518_length_2200_cov_2.332867, whole genome shotgun sequence.
ACCESSION   DAOMPD010000459 DAOMPD010000000
VERSION     DAOMPD010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_2200_cov_2.332867"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLY93_12815"
     CDS             <1..1068
                     /locus_tag="PLY93_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70407.1"
                     /translation="QTIGSAYYRSYWYTDTKSSVSNTFTFTEQFNGDDGGGAYAYRAM
                     NMLMFKDYLFINMGTEQGGGGRGGRICMNPSGCPAGVQFSYVSLPNMYYATRIGALFT
                     PTGGALRNGSFKSNTTGYHGTGADDGILNAINVMYEYDNDGVGGNESQLYFANGGYYS
                     GALTGPRVTNSDGGVIRTKLAYSSKTSLPPNCPNDSTGCVAYYEDITPDAKAEWYKYL
                     SIPYPQNSSASWVDANSDGINDNCASTLVEMNCTLPYNIFVPALKAIPYMRTAPNGDL
                     YMLRNACSTTALNRNGVAGRDFRTEKQVCPKGSEVPQLWMLPKGTTGSPKGAADWVLV
                     AQNGVTNKTNMAGNNAIGANT"
     gene            1083..1352
                     /locus_tag="PLY93_12820"
     CDS             1083..1352
                     /locus_tag="PLY93_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70408.1"
                     /translation="MGNYLYLGWDNATDGANIWRTDMSGMLSGVAPAEGDFSIVNVPG
                     IDGGGGGSNQKIFSHVTVSDAGKDWLIITTRDGADAMKIYRTANP"
     gene            complement(1389..>2200)
                     /locus_tag="PLY93_12825"
     CDS             complement(1389..>2200)
                     /locus_tag="PLY93_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70409.1"
                     /translation="YVAAARRNFTLTADLSITLPWRDEDRLRESKMTVRYAVLPMTAH
                     QRLMGLRATPAVRFSRLDDGAHAAEIMIEQKSTEKTRSYKLQFQATLFNLTHIFNGPV
                     LRQYFKNPTLPEAVRPYVDSLELSPHERTAVEKFRTEWLKRNEGKHPIYAIAAAERDT
                     GLEPSTRCAIYRSLGIPCRRMIFYDLDQKALHTYSQVYIQPTGWVTITPQYSSARPKE
                     FPIANNELELFSPDSVTIHPKPRASTKLSPLPPMSELLKFSNIRIVTSSIE"
BASE COUNT          546 a          504 c          609 g          541 t
ORIGIN      
        1 caaactatcg gctccgcgta ttaccgatcg tattggtaca cggacacaaa gtcgagtgtg
       61 agcaatacgt ttacgtttac cgaacaattc aacggcgacg acggcggggg ggcgtatgcg
      121 taccgtgcaa tgaatatgct tatgttcaaa gattatcttt ttatcaacat gggtacagag
      181 cagggcggtg gcggccgtgg tgggcgtatc tgtatgaatc cgagtggctg tccagcgggg
      241 gtacaattta gttatgtatc gctacccaat atgtactatg caacgcgcat tggcgcgctt
      301 tttactccaa caggtggcgc attgcggaac ggttcgttta agtcgaacac cacgggttat
      361 catggcactg gggctgacga cggcatacta aatgccatca acgtgatgta cgaatacgat
      421 aacgatggcg tgggcggcaa tgaatcgcaa ctttatttcg cgaatggtgg gtattacagc
      481 ggcgctctca ctggtccacg cgtaaccaat agcgatggtg gcgttatccg cacaaaattg
      541 gcgtattctt caaaaacctc gcttccaccg aactgtccta acgatagcac cggctgcgtc
      601 gcatactacg aagatataac tcccgacgcc aaagcagagt ggtataaata tctttcaatc
      661 ccttatcctc aaaattcttc ggcgtcgtgg gtggatgcca atagcgatgg tataaacgat
      721 aactgtgcga gcactctcgt agaaatgaat tgcacattac cgtataacat ctttgtgccg
      781 gcgctcaaag caattccgta tatgcgcacc gcacccaacg gtgatttgta tatgttgcgt
      841 aacgcttgca gcacaacggc gctcaaccgc aacggggttg cagggcgtga tttccgtaca
      901 gaaaaacaag tgtgtcccaa aggctcggaa gtgccgcaac tttggatgct ccccaagggc
      961 acgacgggct cgcccaaagg cgcagcagac tgggttttag tggcacaaaa tggagttacc
     1021 aataaaacta acatggcggg caataatgca ataggcgcta acacttaacg cttttagagt
     1081 ttgtggggaa ttatctttac ttgggttggg ataacgccac cgacggtgcg aacatttggc
     1141 gtacggatat gtccggtatg ctttcgggcg tagcgcctgc ggaaggtgat ttttcgattg
     1201 tgaacgtgcc cggaattgat ggcggtggag gcgggtcaaa ccagaaaatt tttagccatg
     1261 tcactgttag cgatgcgggt aaagattggc tcatcatcac cacccgtgat ggcgctgatg
     1321 cgatgaaaat ataccggaca gcaaatccgt agaaactcgc tgcgagcccg tgcggataaa
     1381 aaattttttc actcgatact cgatgtgacg atgcgtatat tgctaaattt caaaagttcg
     1441 ctcatggggg gcagcggcga taacttggtg gaagcgcgtg gcttggggtg aatggtaacg
     1501 ctatcggggg agaaaagctc aagctcattg ttggcgattg ggaactcttt cgggcgcgcg
     1561 cttgagtatt gcggcgtgat tgtcacccaa ccggtgggct gaatataaac ttgcgaatat
     1621 gtatgcagtg ccttttgatc taaatcgtaa aaaatcatcc gacggcaggg gatgcctaac
     1681 gagcggtata tagcgcagcg tgtggagggc tcgagtcctg tatcacgctc ggcggcagcg
     1741 atggcataga tagggtgctt accttcgttg cgtttgagcc actctgtacg aaatttttcc
     1801 actgctgtcc tttcatgagg cgataattct aacgagtcga cgtagggacg caccgcttca
     1861 ggtaacgttg gattcttaaa atactggcgc aacacaggac cgttaaaaat atgcgtcaaa
     1921 ttgaaaagtg tcgcttggaa ttgcaattta tacgaacgcg ttttctcggt acttttttgc
     1981 tcaatcataa tttctgcagc gtgtgcgcca tcgtcgaggc gtgaaaatcg gacagcgggt
     2041 gttgcgcgta gccccatgag acgctggtgc gctgtcatcg gcaacacagc gtagcgcacg
     2101 gtcattttcg actcgcgcaa ccggtcttca tcgcgccatg ggagagtaat gcttaggtcg
     2161 gcggtgagag taaaattgcg ccgcgctgcc gcgacgtaat
//
LOCUS       DAOMPD010000460         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19530_length_2199_cov_2.214552, whole genome shotgun sequence.
ACCESSION   DAOMPD010000460 DAOMPD010000000
VERSION     DAOMPD010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19530_length_2199_cov_2.214552"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLY93_12830"
     CDS             complement(<1..616)
                     /locus_tag="PLY93_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70410.1"
                     /translation="MAKFDHLEKLWAAYLDYPTARQADKWLVGYFREHKNYGKKDRTW
                     YSEAFFGLLRHALCFLPQEKKCDAREAWAALKSQPLKTLTPSLSQRGIESLPHTWHTW
                     LQKSSLAQEDIPLFLEHLNTRPPLYLRANYPDRASEIEAELKSADFKVETIKQGRLVT
                     FCIEGKTSIYELQCLKNGLIEIQDFASQLLGAAVDAHPRMTVWDI"
     gene            complement(652..2199)
                     /gene="htpG"
                     /locus_tag="PLY93_12835"
                     /pseudo
     CDS             complement(652..2199)
                     /gene="htpG"
                     /locus_tag="PLY93_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
BASE COUNT          470 a          494 c          543 g          692 t
ORIGIN      
        1 aaatatccca caccgtcatg cgcgggtgtg catcgaccgc agcgcccaag agctgtgacg
       61 caaaatcttg gatttcgatg aggccgttct tgaggcattg gagctcatag attgaagttt
      121 ttccttcgat acaaaatgtg acaaggcgtc cctgttttat cgtctcaacc ttaaagtcgg
      181 cagacttcaa ctcggcttca atttcactgg cgcggtcggg atagtttgca cgcaggtaaa
      241 gtggcggcct tgtgttgagg tgttccaaaa atagcgggat gtcttcttgg gctaatgaac
      301 ttttttgtaa ccacgtgtgc caagtatggg ggagactctc aatccccctt tgggatagag
      361 agggagtgag ggttttaaga ggctggcttt ttaatgcagc ccatgcctcc cgagcgtcgc
      421 actttttttc ttgcggcaaa aaacagagcg cgtggcgtag caatccaaaa aacgcttcgc
      481 tataccacgt gcggtctttt ttaccataat ttttatgttc gcgaaaatat cccacaagcc
      541 acttgtctgc ttggcgggcg gttgggtaat ctaagtacgc agcccatagc ttttcgagat
      601 gatcgaactt tgccacaggg ggttagcgct tacggcgttg cagcgccgta actaagaaag
      661 tgattttgac gccaccgcca acatggcttt gttaaacgcg gccacgtctt gcaattcacc
      721 gccatcggcg atgactgcat ggccgtacag gatttgcgcg taatcgtgga cttcactcgc
      781 attgggattt ttatcgtaaa gctggaataa ttttttcacc acttcgtgcg taggatttac
      841 ttcgagtgtt tttttcatcg gcgggacgtt ttagccgttg cgcgctaaaa tttttgcaag
      901 gtacggagag acatcgaact cttcgcccac aagacgtgac ggtgcattca cgagcgcagt
      961 ggtgaattta acttctttta ctgcatcttt gagttgttct ttaagcgctc ctaaaagcgc
     1021 acccatttct ttgtcagctt cttttatttt ttcggcgtct ttcgcttttt ctttttcgtc
     1081 agcgatgtcg agttcacctt tagcggcaga cttaaatttt ttctcgttaa aatcgccgat
     1141 gttctgtaac acgatttcat cgtatgcatc gacgaggtat agaatttcat atcccttcgc
     1201 acgcaacgct tcggtgtgcg gcgaattctc aagcgtcgaa cgggattcgc cagcgatgta
     1261 atagatttct ttttgctcgc tcttcatccg cgaaacgtat tctttgagcg tggtttgctt
     1321 ttctttatcg tgtgacgaag caaagagcaa caacgcagaa agtttttctt tattttcaaa
     1381 atcggtcgat gcgccttctt tgattgcagg accaaactct ttgtaaaagc tggcgtactc
     1441 ctcgcgttct ttttctaaga gcgtggtgag ctcgctgata atttttgtgg tgagaccttt
     1501 tttgatcgca gtgatgtgcc tgtcttgctg caaaatttca cgcgagatat tgagcggtaa
     1561 atctgcgctt tcgatgacgc cgcgcacaaa gcgtaaatag cgaggtaaca gttcttctgc
     1621 tctttcgaga atgagaactt ttttaacgta cagttgtaat ccacctttga agttggtgta
     1681 atataaatcg aacggcgcac gcttgggcag gtataggagc gcttggtatt cgatacggcc
     1741 ttccgcacgg tagttaatta cttttagtgg gttttcccaa tcgtgtgaga ggtgtttata
     1801 gaactcgttg tactcttcct ctttaacatc gtttttggag cgcgtccaaa ttggtttcat
     1861 cgagttgagc gtcgtttctt tgacgacttt ttctgtttta ggttcgccgc tttcaccctc
     1921 tttttgaggg atggtttcgt acacctcttt tttcatcaga atggggtatg cgataaagtc
     1981 agaatatttt ttgacggcgc tttcaacgac aaactcgtcg gtaaaatcgg gcaatccgtt
     2041 ttcattatcg gcgggtttca agtcgagggt gatagtggtg cctgcctgcg gcacgtcggc
     2101 gtcgtcgacg gtgtatgtgc ctgcgtcagt ggaaacccag cgcgttcctt ttgtttcgtt
     2161 tgcgcggcgg gtgagcatcg tgattttatc ggccaccat
//
LOCUS       DAOMPD010000461         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19623_length_2190_cov_2.289930, whole genome shotgun sequence.
ACCESSION   DAOMPD010000461 DAOMPD010000000
VERSION     DAOMPD010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19623_length_2190_cov_2.289930"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLY93_12840"
     CDS             <1..704
                     /locus_tag="PLY93_12840"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRP70411.1"
                     /translation="IVDLVQCKKQIDLVVPRGGSTLIDFVSEHSLIPVVKHDKGVCSI
                     YIAADAPFTMAREIVLNAKTQRPGVCNSVECLLVDAAWPHTKDLMAALQESGVTLHSD
                     AGGIQEFKERGVDVQPFLNDLGFGHEYLSLDASVRFVKNIDAAIEHILTYSSRHSEAI
                     ITQDILQATQFVDALDSAAVFVNASTRFHDGSCFGLGAEVGIATGKLHVRGPMGIKDL
                     TSMRYIVHGQGEIRR"
     assembly_gap    813..824
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <825..1290
                     /locus_tag="PLY93_12845"
     CDS             <825..1290
                     /locus_tag="PLY93_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70412.1"
                     /translation="VLVMPAASAPLRNDNRLFSYRERFHLLRRLFKKEIQEGGVVLSW
                     LEHRLPQPNYTVHTLDTLKKICPQEPTIVIGADQAEKIQHWQRSDFLVQNYTFLIFAR
                     GGVGGDQPLQLVPGMRYTIEKDFNEDISATQVRASLSKLSAQERLDAVEKWG"
     gene            complement(1199..>2190)
                     /locus_tag="PLY93_12850"
     CDS             complement(1199..>2190)
                     /locus_tag="PLY93_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRP70413.1"
                     /translation="TLFVSTTIQFALTIVKILSIAVIVFLLFFFTPATAATAPPTHWP
                     GMAPFAAVLMAAFWAYDGWNNLPMLGGEVKNPQDNLPWALAIGILLVLGVYLALNLSF
                     FRVLSFDEVLAANSGSNRLAEPVATIALAKSVPGASVKIIAALLTISALGAMSGSILS
                     SARVPYAMATDRVFFAALGRLSKKNKTPVAATATQGDIAALLALSGSFDQLTDSVVFA
                     SWIFYALTTAAIFKLRRAVSTGVEPRFKVPLYPFLPIVFLLCASAFILYAMIAMPYLT
                     ARTRRYRVGDSALFLVLEEREITFTPTSPPHPAAPVHSAWRVTHALGLLKYLR"
BASE COUNT          578 a          537 c          587 g          476 t
ORIGIN      
        1 tcattgtcga tttagtacaa tgcaaaaaac aaatcgactt ggtggtgccg cgcggtggct
       61 ctacgctcat tgattttgtt tcggagcaca gcctcatccc tgttgtgaag cacgataaag
      121 gcgtgtgtag tatttacatc gccgccgatg ccccatttac catggcgcgt gaaattgtgc
      181 tcaatgcaaa aactcaacgg ccgggtgtgt gcaatagcgt cgaatgttta ctcgtcgacg
      241 cagcgtggcc gcacactaaa gacttgatgg ccgcgcttca agaaagcggc gtgacgctcc
      301 acagcgatgc gggagggatt caagaattca aagagcgcgg agtggacgtt cagccgtttt
      361 tgaacgactt gggctttgga cacgagtatt taagtttaga cgcgagtgtg cgtttcgtta
      421 aaaatatcga tgccgccatc gaacacatcc tcacgtatag ctcgcggcac tcagaggcga
      481 tcatcaccca agacattttg caagcgacac agtttgtcga cgcactcgat tcggcggcgg
      541 tgtttgtgaa cgcctcgacg cgcttccacg acggtagctg ctttggttta ggcgccgagg
      601 tgggcatcgc caccggcaag ctccacgtgc gtgggcctat gggcatcaaa gatttaacgt
      661 cgatgcgata cattgtgcac ggccaaggcg aaatacgccg ttaaaaaaat tgcaacccca
      721 actgcgtaat ccaccgaaga gagattctcc tctctacttt tttggcggca ctttcgatcc
      781 gccgcaccgt gggcattacc gcatcgtcga gcnnnnnnnn nnnntgtgct cgtcatgccc
      841 gctgcaagcg cgccgctacg taacgataat cgacttttct cataccgcga acgcttccat
      901 ctactgcgtc gcctttttaa aaaagaaatt caagaaggcg gcgtcgttct atcgtggttg
      961 gagcaccgtt tgccccaacc gaattacact gtgcatacac tcgatacgct aaaaaaaatc
     1021 tgtccacaag agccaactat cgtcatcggc gcggatcaag cagaaaaaat tcaacattgg
     1081 cagcggagtg attttctcgt tcaaaattat acatttctta tttttgcgcg cggtggtgtg
     1141 gggggagatc aaccgttgca actcgtgcca ggtatgcgct acaccatcga aaaagatttt
     1201 aacgaagata tttcagcaac ccaagtgcgt gcgtcactct ccaagctgag tgcacaggag
     1261 cggctggatg cggtggagaa gtgggggtga aagttatttc tctctcttcc aaaaccaaaa
     1321 atagagcgga atccccaacg cgataacggc gagtcctagc ggtgaggtag ggcatggcaa
     1381 tcatggcgta gaggataaac gcggatgcgc aaagaagaaa aacgatggga aggaaaggat
     1441 agagtggcac cttgaaacgc ggctctaccc ctgtgctgac agcgcgccgt agtttaaaaa
     1501 ttgccgccgt tgtgagcgcg taaaaaatcc acgaggcaaa aacaaccgaa tcggtgagtt
     1561 gatcgaacga acccgagagc gcaaggagcg cggcgatgtc tccttgggtt gctgtcgctg
     1621 caacgggggt tttattcttt ttagaaaggc gccccagcgc agcaaaaaaa actctatcgg
     1681 ttgccattgc gtacggcacc cgcgcgctcg aaagaataga gccgctcatc gcgccgagcg
     1741 ctgagatggt taaaagagcg gcgataattt tgacgctcgc acccggaaca cttttcgcaa
     1801 gcgcgattgt cgccacaggt tctgccaaac gattgctacc cgaattagcg gcaagtactt
     1861 cgtcaaaact caggacacga aaaaaagaca aattgagagc aaggtataca ccgagtacga
     1921 gaagaattcc gatagcgagc gcccaaggga gattgtcttg tggattttta acttcgccgc
     1981 cgagcatggg caaattgttc caaccgtcgt acgcccaaaa tgccgccatg agcactgcgg
     2041 caaaaggcgc catccccggc cagtgcgtgg ggggtgcagt ggcagcggtc gcaggggtaa
     2101 aaaagaaaag aaggaatacg atgacagcga tactgagtat tttaacgatg gtgagtgcaa
     2161 attggattgt tgtacttaca aaaagagtca
//
LOCUS       DAOMPD010000462         2189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19636_length_2189_cov_2.734770, whole genome shotgun sequence.
ACCESSION   DAOMPD010000462 DAOMPD010000000
VERSION     DAOMPD010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19636_length_2189_cov_2.734770"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLY93_12855"
     CDS             complement(<1..597)
                     /locus_tag="PLY93_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRP70414.1"
                     /translation="MKIKTIFESTTTNSKIVLICLSALCLTPLVSPPIALFMGIIFAQ
                     VFDHPFPETSKKATHWLLKASVVGLGFGMTATSALKAGKEGFLFTVASIFGTLIVGLL
                     LGKLLKVEKITSFLISGGTAICGGSAIAALSPVVKAKQTQISVALGVIFILNSVALFI
                     YPYIGHLLQMSQNDFGLWCAIAIHDTSSVVGAAAKYGND"
     gene            806..1042
                     /locus_tag="PLY93_12860"
     CDS             806..1042
                     /locus_tag="PLY93_12860"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRP70415.1"
                     /translation="MFKRLRLEVPAERVKEPFVFRMVTKYNLQTNIVEANLGPNRSGT
                     VVLEIEGTPQDIERGILFLRETDIEVKEVDVKEK"
     gene            complement(1093..1848)
                     /locus_tag="PLY93_12865"
     CDS             complement(1093..1848)
                     /locus_tag="PLY93_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70416.1"
                     /translation="MAKATPQRKETPAKKSAPKAQKKFFVVPEKTKTPLTKSAYRAPT
                     LGGLPLASKAGLPMIGYRLKKLLINTPNTWEEINREEHGSFFSLFISYHLPLLIPFYF
                     FFVIRELLHFINIILFFRHTLIVLPLFTLFYMGYLFLLSIIAEETAELSGGRFTPQSG
                     VRIAIFSSLILSFLSIATLLPVVGIPFLLLGLFWHYRQLFQGARTLLNIGDNHYKMYR
                     WVHVLVWLLLGLLAFVLLSVVSFLFAKLGHIAI"
     gene            complement(1850..>2189)
                     /locus_tag="PLY93_12870"
     CDS             complement(1850..>2189)
                     /locus_tag="PLY93_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70417.1"
                     /translation="AILDGPRELRLKIPKDLTRENLKVRIFSTAQQFNYTQQDGVLIV
                     HAIPSKKALSVLEVVYKEDGKEYRANVLLNHKRDKSTAKATKVDNKKSTAKNIVRNKK
                     RKKHNVKRNE"
BASE COUNT          684 a          414 c          471 g          620 t
ORIGIN      
        1 atcattaccg tattttgcag cggcgcccac tacagaactt gtatcgtgga tagcgatggc
       61 acaccataat ccaaaatcgt tttgagacat ttgaagtaaa tgaccgatat aagggtagat
      121 aaataaagct actgaattaa gaataaaaat aacgcctaat gccacgctga tttgagtttg
      181 tttcgccttt acaacgggag ataatgcagc gatggcggaa ccgccacaaa tagcggttcc
      241 gcctgaaatt agaaaagatg taattttttc gactttaaga agtttaccca ataaaagccc
      301 tacaatcaaa gttccaaaaa tcgaagcaac ggtaaataaa aagccttctt tccctgcttt
      361 caaggcgctt gtagcggtca taccgaaacc taaacccaca acggaggctt ttaaaagcca
      421 gtgagtagct tttttgcttg tttcagggaa aggatggtcg aaaacctggg caaatataat
      481 ccccatgaat aacgcaatag gaggcgaaac aagtggggtt aagcataaag cggataaaca
      541 gattaaaacg atcttgctat ttgtcgtggt cgattcgaaa atggttttta ttttcatata
      601 atttaaacct ttctaatttt tcttttcttc tattaaattt ttgtagtcaa cgaattgagt
      661 attctctaaa actaaatttg agttgggtcg gtaagtataa ttttttatat ctcacctttg
      721 gtacagaaat gaaatgtctt gacagtagat tgaaaagagt gtgtcgtggg tgaaggcata
      781 aatttcacaa agagaggtga aaagcatgtt taaacgatta cgcttagaag tacctgccga
      841 acgcgttaaa gagccctttg ttttccgtat ggtgaccaaa tataatctac aaacaaacat
      901 cgtcgaagcc aatctaggcc ctaaccgcag cggcacagtg gtactggaaa tcgaaggcac
      961 cccacaagat attgaaagag gaattttgtt cttacgtgag accgatatcg aagttaaaga
     1021 agttgatgtg aaggaaaaat gacactcaga gttaaaaaac agcgaccatt acaattttta
     1081 tgtcggattg atttatatcg ctatatgccc cagtttggca aaaagaaaac tcaccacgct
     1141 caaaaggaca aaagccaaaa gccctaataa taaccacacc aaaacgtgca cccagcggta
     1201 cattttgtaa tggttgtcgc cgatgttgag taatgtacgc gctccttgaa aaagttggcg
     1261 gtaatgccaa aacaacccca aaagtaagaa aggtataccc acaacaggta acaatgttgc
     1321 aatcgaaaga aagcttaaaa tcaacgaaga aaaaatagcg atacggacac cgctttgagg
     1381 ggtaaagcgt ccccctgaca actcggcggt ctcttcggcg ataatactaa gcagaaaaag
     1441 ataccccatg taaaataaag taaagagagg caatacaatc aaagtgtggc ggaagaataa
     1501 aataatattg atgaaatgca atagctctcg gatgacaaaa aagaaataaa aaggaatcag
     1561 caggggtaaa tgatagctaa taaagaggga aaaaaaacta ccgtgctctt cacggtttat
     1621 ttcttcccat gtattggggg tattgatgag tagttttttg aggcgatagc ctatcatggg
     1681 taatccggcc ttggaagcca ggggtaaccc tcccaaggtg ggcgctcgat acgcggattt
     1741 cgtaagcggc gtttttgttt tctcggggac cacaaaaaat tttttttgcg ctttaggagc
     1801 actttttttt gcaggcgtct cttttctttg tggtgtggct ttagccattt tattcattcc
     1861 gcttgacgtt atgcttttta cgcttcttgt tcctgactat atttttggcg gtggacttct
     1921 tgttatccac tttcgttgct ttcgcagtac ttttatctcg tttatggttt aagagtacgt
     1981 tggcacggta ttcttttccg tcttctttgt aaaccacttc tagcacgcta agcgctttct
     2041 tggatggtat tgcatgcacg atgagcacgc cgtcttgttg tgtataatta aattgctgtg
     2101 ctgttgaaaa aatacgcact tttaagtttt cgcgcgttaa atctttaggt atcttcaagc
     2161 gcaattcgcg gggaccgtcg aggatggcg
//
LOCUS       DAOMPD010000463         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19755_length_2179_cov_2.539077, whole genome shotgun sequence.
ACCESSION   DAOMPD010000463 DAOMPD010000000
VERSION     DAOMPD010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19755_length_2179_cov_2.539077"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2179
                     /locus_tag="PLY93_12875"
     CDS             <1..>2179
                     /locus_tag="PLY93_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70418.1"
                     /translation="CTYKEFHLPNILVQLLNGINDHGMDILIDSVRHSYTNLACISNP
                     IANADVPTANYMASNQALCPESAMITTQPATTAAADTFASGQPYKVKVDHYTFLMKLA
                     YIVAGFDYSDFTSGTDKNFAAPNLPGPAKLYNLMNLTTDARDMVFLLDSFDRETCPGT
                     GNCIAVNADNTITLVLPYSTGDLWTDIQGNQQFMGLKNLLSVLDLVTDTSKMGMLVNG
                     YRPTTGRFDTSYDSGQIRYYLDRLRVVIERRTTCNAASYPGTPAEQALQDFTVPQGGA
                     AAWNQKLAKLINLISLANTNRMMDLIYYIDDGFDINHLETGSCPNRGIDNLMVMLNNL
                     NDVAPHVNDMATYNPPYTNLTPNTTNSELITAASLIENVNLDPTNDNPAKRNRVKYLI
                     EYIGNTLDVLQLACYTAITDNNPTPAGTCANRGLINQVADGSKLSDTSTVALSAAGQK
                     MAALMNYSAGPPEQGIADIEKMRFLVKNVSMTNLNGMIKGMLIAGTQKTADVVNMITG
                     NNCWDTSNGVIGVLDGITSLPINPAAGIGSGYTSPPTVSISGGGAGAIAKAVIETDST
                     KSGYQTIKDVIVLSNGTGYAGGNITFVGGGGIPPGTNPTNIVGNCNFTPPITYRGFPT
                     ATGTGATGMGKLVNMINYVTGSPKSITDLINGVNDGNKLGLLINGIGRSSNLVGVMNA
                     VLDANRNNNATKQDLIDLVNNLSREDILKMVHLLENLGDAREVD"
BASE COUNT          595 a          555 c          519 g          510 t
ORIGIN      
        1 ctgtacttac aaagaatttc atttaccgaa catcctggtg cagcttttga atgggattaa
       61 cgaccacggc atggatatac tcatcgattc agtaaggcat agttatacaa acttagcgtg
      121 catctcaaat ccgatcgcta atgccgatgt accgacggcg aattacatgg cgtcgaacca
      181 agcgctttgt cccgagagcg caatgattac cacccaaccg gcgacaaccg ccgcagcgga
      241 tacttttgcc agcggtcaac cgtataaagt aaaagtggat cactatacct tcctgatgaa
      301 gctggcgtat atcgtcgcag ggttcgatta ctctgatttc acgagcggga cggacaaaaa
      361 ttttgctgca cccaatttac caggtcccgc taaactctat aatttgatga acctcactac
      421 ggacgcgcgc gatatggtct ttctccttga ttccttcgat cgtgaaactt gtccgggcac
      481 ggggaattgt atcgccgtta atgcggacaa caccatcacg cttgttctcc cgtacagcac
      541 cggtgattta tggacggata tccaagggaa tcaacagttt atggggctca aaaatttgtt
      601 gagcgtactg gatttagtaa cggacacttc taaaatgggt atgttggtga acggctaccg
      661 acccacaacc ggtcgctttg acaccagtta cgatagcgga cagattcggt attatctcga
      721 tcgcttgcgc gtggtgatcg aacgacgcac gacgtgtaat gcggcttcgt acccaggcac
      781 cccagccgaa caagcgttgc aggattttac agtaccgcaa gggggggcgg ctgcgtggaa
      841 ccaaaaactg gccaaactca tcaatctcat ctcgctcgcg aacaccaacc gtatgatgga
      901 cctcatctat tatatcgacg acgggtttga tatcaaccac ctcgagactg gctcatgccc
      961 caaccgtggc atcgacaacc taatggtgat gctcaacaac ttgaacgatg tcgctccgca
     1021 cgtaaacgat atggcgacgt ataacccgcc gtacaccaat ttgacgccca acaccaccaa
     1081 cagcgaactc atcaccgccg cgtctctcat cgagaatgtg aaccttgacc caacgaacga
     1141 caaccccgcc aagcgcaacc gcgtaaaata cctcattgaa tacattggca atactttgga
     1201 tgtgcttcag ttggcgtgct atactgcgat taccgataac aaccccacac cggcgggaac
     1261 gtgcgccaac cgtggcctta tcaaccaagt tgccgatggg tctaaactaa gcgacacttc
     1321 aactgtcgct ttatcggcag cagggcaaaa aatggccgcg ctcatgaatt attctgctgg
     1381 tccaccggag caaggcattg ccgacattga aaaaatgcgc ttcttggtca aaaacgtttc
     1441 aatgacgaat ttaaacggca tgatcaaagg gatgctcatt gctggtacgc aaaagaccgc
     1501 cgatgtggtg aatatgatta ccggcaataa ctgttgggat acctccaatg gtgtcatcgg
     1561 tgttttagat ggaataacca gcttaccgat taacccagca gcaggtattg gttcaggtta
     1621 taccagtcca cccactgtat ccatttcagg ggggggcgca ggcgctatcg cgaaagcggt
     1681 gattgaaacc gattcaacaa aatcaggata ccagacaata aaagatgtaa tcgttttatc
     1741 gaatggaacg ggctacgctg gtggaaatat tacttttgta ggcggcggtg gaattccccc
     1801 tggaacaaat cccacaaaca tcgtgggcaa ctgtaatttt acaccaccca taacgtaccg
     1861 tggttttccc acagccaccg gcaccggtgc caccggcatg ggtaaacttg tgaatatgat
     1921 taactatgtg acgggttcgc ccaagtcgat caccgatctc atcaacggtg tgaacgatgg
     1981 taacaagctg gggctcctta tcaacgggat tggccgttcg agtaatcttg tcggcgtgat
     2041 gaacgcggtg ctcgatgcaa acagaaataa taacgccacc aaacaagatt tgattgatct
     2101 tgtgaacaat ctttcgcgtg aagacatttt gaaaatggtg caccttttgg aaaatttggg
     2161 cgatgcgcgc gaggtggat
//
LOCUS       DAOMPD010000464         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19786_length_2176_cov_2.021688, whole genome shotgun sequence.
ACCESSION   DAOMPD010000464 DAOMPD010000000
VERSION     DAOMPD010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19786_length_2176_cov_2.021688"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLY93_12880"
     CDS             <1..554
                     /locus_tag="PLY93_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRP70419.1"
                     /translation="QQKINEHKAGKTFRVWSAAASSGQEAYSIAMLLAENFGMSGWEI
                     VGSDISERILDVAINATYPIEQASQVSDVYLKNYCLKGVNASEGKFCIRPEILRNVRF
                     LRVNLNENIPQSLGLFDVIFLRNVMIYFDKPTRQQVAERLAKFLKPHGNLVIGHAESL
                     HGITTVLKTVKPTIYAIATTTHD"
     gene            526..1062
                     /locus_tag="PLY93_12885"
     CDS             526..1062
                     /locus_tag="PLY93_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRP70420.1"
                     /translation="MRLQQLPTTDTAPSVYEVYLQPGEYYWGDETHRIKTLLGSCVAI
                     CVWHPLRKIGGMSHCLLPSRVVDGVWRGVSREISKNEWSGRYVDETFMIFFDLMRQHA
                     TLPQEYQVKVFGGGNMFELQDKNSITVGERNIEMIHTILEREKIPIVAQHMGGNGHRN
                     IIFELWNGGVWMRHTEIP"
     gene            1068..1679
                     /locus_tag="PLY93_12890"
     CDS             1068..1679
                     /locus_tag="PLY93_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HRP70421.1"
                     /translation="MTAFTPPTIPLRRFIAIGSSTGGPQALETVLQGVSKLTLPIVIT
                     QHMPENFTFAFANRLNDLLSITVKEAEDGDTLQMGYVYVAPGGKQMRVKRNKADFFIH
                     LSDEPPVNRHRPSVDVLFDSVVYAAPDAALGVLLTGMGNDGAAGLLRMREKGYLTIAQ
                     DEASSVVWGMPREAIKLGAVEEKNILPLDKIAAAMMAFVNKRM"
     gene            1716..>2176
                     /locus_tag="PLY93_12895"
     CDS             1716..>2176
                     /locus_tag="PLY93_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRP70422.1"
                     /translation="MAYDKIKLPAKGEKIAVKDGKLNVPENPIIPFIEGDGTGPDIWK
                     ASVRVLDAAVAKAYAGKRKIEWLEVYAGEKSNEVYAPNTWLPDETLTALADYKVSIKG
                     PMTTPVGGGIRSINVALRQMLDLYTCLRPVQYFDGVPSPVKHPEDVEMVIFR"
BASE COUNT          575 a          514 c          547 g          540 t
ORIGIN      
        1 tgcagcaaaa aataaacgaa cataaggccg gtaaaacttt ccgtgtgtgg agtgccgccg
       61 cctcaagcgg tcaagaggcg tatagcatcg ccatgctgtt ggcggagaat tttggcatga
      121 gcggttggga aattgtcggc tccgacatca gcgaacgcat tctcgatgtc gccatcaacg
      181 ccacttatcc catcgaacaa gcatcgcaag tttccgacgt ctacttaaag aactattgcc
      241 tcaaaggggt aaacgcgtcg gaaggaaagt tttgcatccg accagagatt ttacgtaacg
      301 ttcgtttctt gcgtgtgaac ctaaatgaga atataccgca atcgttggga ttgtttgacg
      361 tgattttttt acgcaatgta atgatttatt ttgacaagcc cacgcgccaa caagtcgccg
      421 aacggctcgc aaaatttttg aagccgcacg gcaatttagt gattggtcac gcggaaagtt
      481 tacacgggat caccactgtg ctcaaaacgg taaagccgac aatttatgcg attgcaacaa
      541 ctacccacga ctgataccgc accgtccgtt tatgaagtgt atttgcaacc cggtgagtat
      601 tactggggcg acgaaacaca ccgtataaaa actctgttgg gctcttgcgt agctatttgt
      661 gtgtggcatc ctttgcgtaa aattggcggt atgagccact gtcttttacc ttcgcgcgtt
      721 gtcgacgggg tgtggcgggg agtgagtcga gaaatctcca aaaacgagtg gagtggtcgt
      781 tacgttgatg aaacgtttat gatttttttt gatttgatgc ggcaacacgc cacgctacct
      841 caagaatatc aagttaaagt ttttggtggc gggaatatgt tcgagctgca agataaaaat
      901 agcatcaccg tgggcgaacg gaatatcgaa atgatacaca caattttaga acgcgaaaaa
      961 attcccattg tcgcgcaaca catgggtggc aatgggcacc ggaatattat ttttgaactc
     1021 tggaatggcg gcgtttggat gcgccatact gagataccgt aactgttatg actgccttca
     1081 cgccaccgac aatccctctt agacgattca ttgccattgg ttcttcaacg ggggggcccc
     1141 aagcgctcga aacggtattg caaggcgtga gcaaattaac tttgccgatt gtcattacgc
     1201 aacacatgcc cgaaaatttt acattcgcgt ttgcgaaccg tctcaacgac cttttgtcga
     1261 tcacagtgaa agaagccgaa gacggcgaca cgttacaaat ggggtatgtg tatgtcgccc
     1321 ctggtggtaa acaaatgcgc gtcaaacgca acaaagcaga tttttttatt catctttctg
     1381 acgagccacc ggtcaaccgc catcgccctt cggtcgatgt cctttttgat tctgtcgttt
     1441 acgctgctcc tgatgcggcg ttgggggttt tactcacggg catggggaat gacggcgcag
     1501 ccggtctttt gcgtatgcga gaaaaaggtt atctcaccat tgctcaagac gaagcgtcgt
     1561 cggtggtgtg gggtatgccg cgcgaggcaa ttaaattagg ggcagttgaa gaaaaaaata
     1621 ttttaccgtt agataaaatt gccgcagcaa tgatggcgtt tgtcaacaaa cgaatgtaaa
     1681 actttacacc gcatttcaag catcgagctt gtctaatggc atacgataaa attaaactac
     1741 ccgcaaaggg agaaaaaatc gccgtcaaag acggcaaact gaacgtcccg gaaaatccta
     1801 tcattccttt tattgagggc gacggcaccg gcccagacat ctggaaggca agcgtgcgtg
     1861 tactcgacgc ggcggttgcg aaagcgtatg ccggtaagcg caaaattgaa tggctcgaag
     1921 tttacgccgg cgaaaaatca aacgaagttt atgctcccaa cacatggttg cccgatgaaa
     1981 cactcaccgc ccttgccgac tataaagttt ccatcaaagg cccgatgacg acccccgttg
     2041 gcggcggcat ccgttcgatc aatgtggcgc tccgccagat gctcgatctc tacacgtgct
     2101 tgcgccctgt gcagtatttc gacggcgtgc cctcgcctgt caaacatcct gaagacgtag
     2161 agatggtcat ctttcg
//
LOCUS       DAOMPD010000465         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19824_length_2172_cov_2.329712, whole genome shotgun sequence.
ACCESSION   DAOMPD010000465 DAOMPD010000000
VERSION     DAOMPD010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19824_length_2172_cov_2.329712"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLY93_12900"
     CDS             <1..535
                     /locus_tag="PLY93_12900"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRP70423.1"
                     /translation="SSFKNELTDEVVRSGFFNFLKSKNVMLVKSAEESRALVEMQGPI
                     IVVSASGMLSGGRVMHHIEHRLGDTRNYVLFTDYVGEGTLAHQILSGQTSINIYGKEI
                     PIRAHVAQIHSFSAHADLNALLSWMHHFQKNNVERIFINHGEDAARENLAKELSFMRG
                     AKIELPRYQSTYYLTGV"
     gene            complement(517..>2172)
                     /locus_tag="PLY93_12905"
     CDS             complement(517..>2172)
                     /locus_tag="PLY93_12905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRP70424.1"
                     /translation="DNLKVGVVRDWANKIITDENLTGHRFEDAPKLPESIWSLITRDS
                     DGCPATRCQHYANCNYYRARKKWGEAQLVVANHHLFLYHLLNDKYTLPDYGALVIDEA
                     HGLIKTGFNIFTATFGRHSFADLKKGIDKTLLQQLGVQGEIRLELEELVNTCLTRWET
                     FFSQWENETNLTFTETGTELIRKNLSSAPELSPPLTELKEKFEDLRSQDLESTLLNAV
                     NAQYKELRKMDEFAKLFTAFNTTHNVYWAEKKNERFYLNACRLNLGETLANLIPELRL
                     YTSATLGYLPMGDFPTKKSELIQRGFFNRFLTDALPLAEEGSIDCNVFFSPFNYREHA
                     ALYIPDKFMLPEHGAPFAVLNQYFDDLTQEIAALTQMSSGGTLVLFTSNYHLSQVTDR
                     LRAATDLPVISQLEIGVQDALAQFRKDKNSILLGSQSFWQGIDIAGDNLRLLIITKLL
                     FTPPDDPIFKARSENLEAQGKKPFFELSLPHATTMLRQAFGRLIRTESDKGVVALLDS
                     RVWKKSYGKTLLSNLPRVTATKTFSELEQANKKFGLLHYTPVK"
BASE COUNT          517 a          505 c          581 g          569 t
ORIGIN      
        1 ctcgtctttc aaaaatgaac tcaccgacga agtagtgcgc tccggttttt ttaatttctt
       61 gaaatccaaa aacgtgatgc tggtgaaaag cgccgaggag agccgcgcgc tcgtcgaaat
      121 gcaagggccg attattgtgg tgagcgcgtc ggggatgctc tcgggtgggc gggtgatgca
      181 ccacatcgaa caccgtttag gcgatacgcg caactacgtg cttttcacag actacgtggg
      241 cgagggcact ctcgcgcacc aaattctaag cggccaaacg tcgattaaca tatacggcaa
      301 agaaatcccc atccgtgcgc acgtcgcaca aatccactcc ttctctgcgc acgccgactt
      361 aaacgctttg ctttcgtgga tgcaccactt ccaaaaaaat aacgttgagc gtatttttat
      421 caaccacggc gaagacgccg cccgtgaaaa tttagccaaa gaactgtcgt tcatgcgcgg
      481 cgcaaaaatt gaactccccc gttaccagag tacgtattat ttgacagggg tgtaatgcaa
      541 cagaccaaac tttttatttg cctgttccaa ctctgaaaat gttttggttg ccgtcacgcg
      601 cggcaaattg gagaggagtg tcttgccata gctctttttc cagacgcggc tgtcgaggag
      661 cgccaccacg cctttgtcgc tttcggtgcg gatgagcctc ccaaaagctt ggcgcagcat
      721 cgtcgttgcg tgtggaagcg ataattcaaa aaatggtttt ttaccctgcg cttccaaatt
      781 ttcagaacgt gctttgaaga tagggtcgtc gggtggcgtg aaaagaagtt tggtaataat
      841 aagaaggcgc aagttgtcgc ctgcgatgtc gatgccttgc caaaacgatt gcgaacccaa
      901 caagatggaa tttttgtctt tgcggaattg ggcgagcgcg tcttgcaccc caatttccag
      961 ttgtgaaatc acgggaaggt cggtggccgc acgcaagcgg tctgtcactt gcgacaagtg
     1021 gtagttcgag gtgaaaagca ccaacgtgcc ccccgatgac atttgggtga gcgcagcaat
     1081 ctcttgagtg agatcgtcga agtattggtt tagcacagcg aagggtgcgc cgtgttcggg
     1141 tagcatgaac ttgtcgggta tgtaaagcgc ggcgtgttcg cggtaattga agggagagaa
     1201 aaacacattg caatcgatgc tgccctcttc ggcgaggggt agggcatcgg ttaaaaagcg
     1261 gttgaagaaa ccgcgttgga tgagttctga ttttttggtg gggaaatctc ccatgggtaa
     1321 ataccccaac gtcgccgaag tgtacagtct gagttcgggg attaaattgg ccaacgtctc
     1381 gcccaagttg aggcggcacg cgttcaagta aaagcgctcg ttcttttttt ccgcccagta
     1441 cacattgtgt gtggtgttga acgcagtgaa gagtttggca aattcatcca tcttgcgcaa
     1501 ctctttgtat tgtgcgttca ctgcgtttaa gagagtgctc tccaaatctt ggcttcttaa
     1561 atcttcaaac ttttctttga gttcggtcaa agggggactc agttcgggcg cggacgataa
     1621 atttttgcgg atgagttctg tacctgtttc ggtgaacgtc aaattcgttt cattttccca
     1681 ctgcgaaaaa aaggtttccc aacgagtaag gcaagtgttc acgagttctt cgagttcaag
     1741 gcggatttct ccttgcacgc ctaactgttg cagtagcgtt ttatctattc cctttttcaa
     1801 atcggcaaag ctgtggcggc caaatgtagc ggtaaaaata ttgaatcccg ttttgatgag
     1861 cccatgcgct tcgtcgatga caagcgcgcc gtaatcgggg agggtatatt tatcgttcaa
     1921 taaatgatac aagaaaagat ggtggtttgc cacgacgagc tgcgcttctc cccatttttt
     1981 acgcgcacgg taataattgc agttggcgta atgctggcag cgcgtcgcag gacaaccgtc
     2041 gctgtcgcgc gtgatgagcg accatataga ctcgggcagt ttgggggcgt cttcaaaacg
     2101 gtggccggtt aaattttcat cggtgataat cttgtttgcc caatcgcgca ctacgcccac
     2161 tttgaggttg tc
//
LOCUS       DAOMPD010000466         2162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19942_length_2162_cov_2.308495, whole genome shotgun sequence.
ACCESSION   DAOMPD010000466 DAOMPD010000000
VERSION     DAOMPD010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19942_length_2162_cov_2.308495"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLY93_12910"
     CDS             <1..846
                     /locus_tag="PLY93_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70425.1"
                     /translation="IQVNGDSWGTSNQRYNQLNVEGLVVHSSAVGKGRIPMVRLLTGQ
                     DLKIVLSWGAANPFDPDIHLVGTVPAGQSITNVNGDNCNTDHFHVWAARPNVGLSWQQ
                     QYSAKTRTYIQGDASYNGANYFPVSSSTTVALVQDANSGYGPEAINMLSGYTDGTYWV
                     TVINWGQWYDSSYSVTKASQRWDVTQVGLKVYDANGLAFEMIAAQPTTAPTALAPATL
                     VAGCGAGGASDWNECELWQAFKITVAGGGAAGRKFTPVNTYQNWQDRSGANVYDMNKC
                     YLKTF"
     gene            858..>2162
                     /locus_tag="PLY93_12915"
     CDS             858..>2162
                     /locus_tag="PLY93_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70426.1"
                     /translation="MRHKTYPRLALFLTTLTLLFTTPACVGRFLSTMLSNEGADFKII
                     EASAMANTRIRVSFNRPLDKTQGASTVLSNYSIPGLTLISVEAGSESNQVFLNLDSSE
                     TTASNRMQHKSYTLTVSNVLNQTLDPLLYEGRSISFMGARWLRAVCQNDDGTSSCPPL
                     AATGGATGYEIKVRGDYAVGTNYLWRLVDPSTSTPKPACENPMHTNHPTCNDGALPTY
                     DNGLGSGTTTTLNWNATPIPVSTPIRIIGLLTGNYRLELMIQDAQGAWQPMSEQTAYD
                     FFVDGTSPERIGLSNAGGPSSVPAYTTLTNVQITNIGIRPRSCTEFPNPAPDCSASPE
                     APSHYKYQILYQAGSCPSALPTVLVPWTNEISANIPITYDMSANGEGCYTIRVLARDI
                     AGNWQCDTSAGDTAINRCQHNLGDKSVNDFFNNRAYQVAHITF"
BASE COUNT          552 a          554 c          546 g          510 t
ORIGIN      
        1 attcaagtca atggcgacag ttggggaact agtaaccagc gctataacca actcaacgtt
       61 gagggacttg tggtacacag cagcgctgtc ggcaaaggac gtatacccat ggtgcgcctc
      121 ttaaccggcc aagatttgaa aatcgttctt tcgtggggag ctgcaaatcc cttcgatccg
      181 gatatacatc ttgtgggtac tgtgccagca gggcaaagta tcaccaatgt gaatggagac
      241 aactgcaaca ccgatcactt tcatgtttgg gcggcgcgcc ctaacgtcgg tttaagctgg
      301 cagcagcagt actcggcaaa aacccgcaca tacatccaag gggacgcttc gtataatgga
      361 gccaactatt tcccggtaag ttcatcgacc acggttgcgc tcgtgcaaga cgcgaattca
      421 ggctatggcc ccgaagctat caatatgctt tcggggtaca ccgacggcac gtactgggtg
      481 acggtgatta attggggcca atggtacgat agttcgtata gtgtcacaaa ggcgtcgcag
      541 cggtgggatg tcactcaggt aggcttaaaa gtgtacgatg ccaacggtct tgcgtttgaa
      601 atgattgcag cacaaccgac aacggcacca acagcgcttg caccagcgac acttgttgcg
      661 ggttgcggcg cagggggggc gtccgattgg aacgaatgcg aattatggca ggcgttcaaa
      721 attaccgttg cgggcggtgg ggcagcggga cggaaattta cccctgtcaa cacctaccaa
      781 aattggcaag accgttcggg agccaatgtg tacgatatga ataaatgtta tctcaaaaca
      841 ttttgaggaa aaaaattatg aggcataaaa catatccacg gttagctctc tttttaacca
      901 cgcttacgct gctttttact actccggctt gcgtggggag atttttatcg acaatgctct
      961 ccaacgaagg cgcggatttc aaaattattg aagccagcgc gatggccaac acccgcattc
     1021 gggtttcgtt taaccggccg ctcgacaaaa ctcaaggggc gagcacggtc ttgagtaatt
     1081 atagtatccc cggtttaacg ttgatttcgg tggaagcggg ctctgaatcc aaccaagtgt
     1141 ttttgaatct tgattccagc gaaacgacag ccagcaaccg catgcagcat aaatcgtata
     1201 ctctcactgt atcgaatgtg ctcaaccaaa cattagaccc acttttgtac gagggacgct
     1261 cgattagttt tatgggtgcg cgctggttgc gtgcagtatg ccaaaacgac gacggcactt
     1321 cgtcgtgccc gcctttggcg gcaacgggcg gtgcaacggg ttatgaaatt aaagtgcgtg
     1381 gcgattacgc tgtaggtacc aactacctct ggcgtttagt tgatccttcc accagtaccc
     1441 ctaaaccagc ttgcgaaaac ccaatgcaca ccaatcaccc aacctgtaac gatggcgcct
     1501 tgcctacata cgacaatggt ttgggtagtg gtacgacaac gacgttgaat tggaacgcaa
     1561 cccccatccc tgtaagtacc cccatccgta tcatcggtct tctcaccggg aattaccgtt
     1621 tagagctaat gattcaagac gcccagggtg cttggcaacc gatgagtgaa caaacggcat
     1681 acgacttttt tgtcgatgga acctcgccgg aaaggattgg attaagtaat gccggcggtc
     1741 cttcatcggt acctgcgtac accacgctca ccaacgtgca aattaccaac attgggattc
     1801 gccctcgtag ttgcactgaa ttccccaacc ctgcacccga ttgttctgcg tctcccgaag
     1861 cgccgtcgca ttataaatac caaattctct accaagcggg tagctgcccg agcgctctac
     1921 ctacagtgct tgtgccttgg acgaatgaaa tttcggcgaa catccccatc acctacgaca
     1981 tgagcgccaa cggtgagggt tgctatacca tccgtgttct tgcgcgagat attgcgggta
     2041 attggcagtg cgataccagc gcgggtgata cagcaatcaa caggtgccag cataatttgg
     2101 gcgataaaag cgtgaatgac ttttttaata atcgcgctta ccaagtcgcg catatcacct
     2161 tt
//
LOCUS       DAOMPD010000467         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_19977_length_2158_cov_2.842606, whole genome shotgun sequence.
ACCESSION   DAOMPD010000467 DAOMPD010000000
VERSION     DAOMPD010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19977_length_2158_cov_2.842606"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PLY93_12920"
     CDS             complement(<1..1461)
                     /locus_tag="PLY93_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRP70427.1"
                     /translation="MRGKMPILFSLLVSFLIVGCYVGSVGDEAPNQGALQQTAQGQIV
                     ANLTNKGIVKDARLNEQSMIALYIYRKKDYSLSCTTATNPKKCEDLLIFKNEKLDALG
                     FTNEPGYFGYDADFDSYSPMLVAEGNSYPYATKPADPPGFPDPDVWQCRKITYPSSPD
                     YGKYECGVNSHPIRNFKKDLTTYTLQGYATNGRGYASSYILDYNLFNSLNHPVKDGDR
                     FYAYFAVHFKAIKAARDSEDRFDKTTSTWKRPIEPDLISPRIEINMKMLAEGAYDKPG
                     SVYDIPDYATWYDPFAGCRPTTGVAGSVYFSEILWMGSKSIAAVSATEDEFIELYNNT
                     GAAINISGWKIKGAATGGGDLVLPLCATIPANGVYTVGKQTTKAFTQFDYTASGISLS
                     NSGVNLVLEDAQGTTIHTMTSCTSPAWGGRGVNGGANTPKRSMRLAAIATASPLCTAW
                     VTTSTGDTNYPTASANVSALYAAPDDVSEGTVATPGY"
     gene            1557..>2158
                     /locus_tag="PLY93_12925"
     CDS             1557..>2158
                     /locus_tag="PLY93_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRP70428.1"
                     /translation="MQTVIKEADIIESTAAALQYISYYHPPDFIQAMKVAYEKEESRA
                     AKDAMAQILLNSRMSAFGHRPICQDTGIVIVFIELGMDVRIEGTLATLEDMINEGVRR
                     AYLDHDNILRASIVADPAGARKNTKDNTPAVIYTRLIPGNTMDITVAAKGGGSENKSK
                     FTMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGRAD"
BASE COUNT          515 a          553 c          547 g          543 t
ORIGIN      
        1 gtatcctggg gtggccaccg tgccttcgct cacgtcatcg ggcgcggcgt acaatgcact
       61 cacattcgct gaagcagtcg ggtagtttgt gtcgcccgta ctcgtggtca cccaagcggt
      121 gcacaacggc gatgcggtgg cgattgccgc taaacgcatc gagcgtttgg gagtgttggc
      181 tccaccattg acgccccttc ccccccacgc aggtgacgta caacttgtca ttgtgtggat
      241 tgtagtgcct tgcgcgtctt cgagcaccag gttgacgccg ctattcgaaa gcgatatacc
      301 ggaagccgtg taatcaaact gggtaaacgc ttttgtggtt tgtttcccaa cggtgtaaac
      361 accattggcg gggattgttg cacataaagg taaaacaaga tctcctcccc ccgttgccgc
      421 tcctttgatt ttccagcctg aaatattgat agcagcgccg gtgttgttat agagttctat
      481 aaattcatct tctgtggccg aaacggcggc aatgctctta ctccccatcc acaaaatttc
      541 actaaagtag acgcttcccg ccactcctgt tgtgggacgg caaccggcga aggggtcgta
      601 ccatgtcgca taatcgggta tatcgtacac gctgcctggt ttatcgtacg caccttcggc
      661 gagcattttc atgttgattt caatgcgcgg cgagataagg tcgggttcga tggggcgttt
      721 ccacgtcgaa gtggttttat caaaccggtc ttcggaatcg cgcgccgctt tgatggcctt
      781 aaaatgcacc gcgaaataag cgtagaaccg atcgccgtct ttaacgggat ggtttaagct
      841 attaaaaaga ttgtaatcta aaatgtacga tgaggcatag ccgcgcccgt tggtggcata
      901 gccttgcaag gtgtacgtgg taaggtcttt cttaaaatta cgtatggggt gagaattaac
      961 cccacattca tatttaccat aatccgggct gctcgggtac gtaattttgc ggcactgcca
     1021 cacgtcgggg tcgggaaatc ccggcggatc cgccggtttc gtagcgtagg gataactatt
     1081 cccctcggcg acgagcatgg gggagtaact atcaaaatcc gcatcgtaac caaaataacc
     1141 gggctcgttg gtgaatccca atgcgtcgag tttttcattt ttgaaaatga gcaagtcttc
     1201 gcatttttta ggattcgtgg cagtggtgca actcaagcta taatcttttt tacggtagat
     1261 ataaagagcg atcatgcttt gctcgttcaa acgcgcgtcc ttgacaatcc ccttgttggt
     1321 gagattagcg acaatttgac cttgcgcggt ttgttgcaat gcgccttggt tgggggcttc
     1381 atcgcccacc gagccgacgt agcaacccac tattaagaag cttaccaata aagagaagag
     1441 aatgggcatt ttgcctctca tggactcaat ggcgcttatc atcgatgctt tggcaagcat
     1501 tttagaattt atgcaaagtt actttccgct ctcattcctt ttattatatt agcactatgc
     1561 aaacagtaat taaagaagcc gacattatcg aatccacagc cgctgcgctg caatacatct
     1621 cgtactacca tcctcccgat tttatccaag cgatgaaagt tgcttatgaa aaagaagaat
     1681 cccgcgccgc caaagacgcg atggcacaaa ttctattgaa ctcgcgcatg tccgcatttg
     1741 gccatcgacc catctgccaa gacaccggca ttgtgatagt gtttatcgaa ctgggcatgg
     1801 atgtacgcat cgaagggaca ttggctacgc ttgaggatat gatcaacgaa ggcgtgcgcc
     1861 gtgcatacct cgaccatgac aatattttgc gggcgtcgat tgtcgccgat cccgccggag
     1921 cgcggaagaa cacaaaagac aacacccccg ccgtcattta tacccgcctt atccccggca
     1981 acacaatgga tatcaccgtc gcggcaaaag gcggtggttc cgaaaataaa tccaaattca
     2041 ccatgctcaa tccttccgac agcatcgtcg actgggtact caaaacagtg ccgacgatgg
     2101 gcgcgggctg gtgcccgccg gggatgctcg gcatcggcat cggtgggagg gccgacaa
//
LOCUS       DAOMPD010000468         2151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20048_length_2151_cov_3.990935, whole genome shotgun sequence.
ACCESSION   DAOMPD010000468 DAOMPD010000000
VERSION     DAOMPD010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20048_length_2151_cov_3.990935"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLY93_12930"
     CDS             <1..201
                     /locus_tag="PLY93_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70429.1"
                     /translation="RDAFLESKFHLKENAGKEAFVDMYDDLLRVGVDLFDFQVYSPIA
                     ASFGGYEIPREIYGIYLEEALR"
     gene            201..>2151
                     /locus_tag="PLY93_12935"
     CDS             201..>2151
                     /locus_tag="PLY93_12935"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HRP70430.1"
                     /translation="MAFYYYQSTDFNVLFNRIYALVKEERKALKPQTPQTIVVGSRGI
                     TEWLEEKFIERDDVLMGIDFSFLENALLAFDERANTQSFPEANESWFQAPQQSDELSY
                     ATFFDFQLAVLDVLLTSQKKEDALQKEFNLKNLTQLQMLALLQKVTKQINEYLLHAPE
                     AMQKALTQRNPPPTLQLFSLAHEKLTQRGLISPLFLLSPQKKESFPFKKSSTPKVSHT
                     LYLFGMPLLSAYHLTQLLRVSQNVDVHLAALHIPFIKEGKNLFYQKASQHFEQYTDFL
                     QKKASALNATLHIESTESTLSLWQKEWASGASSQTFVDKRIEFLALPATYRGAEIIAD
                     KWHDALVKDDTLRQEDFSLLLTDEKNQFAAFEKASHERKLSLFTRAKFFERVSDVLEL
                     LSIFSQASSEGITRELVVTYLNNPLVCETFALTRETQDLYIAALSSANGYRSNYEASL
                     AIYNFDAAIKRLRLGILVDTQNATDEEVPGAAYLREFDSVEIFENFLNCIGLLLDTPS
                     ALQNQSAKKITEQFVKLITRFESPNNPFEIAEVCSTLKKIELALEEHEMSAAQIVSLL
                     TNTLSKPSLTQKYDELGICASSFLSVTPHRRHTTIFSLNEEIDKDTKLDADALPEYSL
                     SPTRLEKRDRTQIELVQAALSPCEVL"
BASE COUNT          634 a          527 c          411 g          579 t
ORIGIN      
        1 cgcgacgctt ttttggaatc gaagttccac ttaaaagaga acgcgggtaa agaagcgttt
       61 gtcgatatgt atgacgattt actccgtgta ggcgttgatc tttttgactt ccaagtgtac
      121 tcaccgattg ccgcgtcttt tggcggctac gaaatcccgc gtgaaattta tggaatttat
      181 ttagaagagg ctctccgcta atggcttttt actactacca atcaaccgac tttaacgttc
      241 ttttcaacag aatctacgcc ctcgtcaaag aggaacgtaa agcattaaag ccgcaaacgc
      301 cacaaaccat cgtcgttggc agtcggggta tcaccgaatg gcttgaagaa aaatttatcg
      361 agcgcgacga cgtgttaatg ggaattgatt tctcattttt agaaaatgct cttttagcat
      421 ttgatgaacg cgcgaatacg caaagctttc ccgaggccaa cgaatcgtgg ttccaagcgc
      481 cgcaacaaag cgacgagctt tcttatgcga cgttctttga tttccaactg gcagttttag
      541 acgttttatt gacttcacaa aaaaaagaag acgcgctcca aaaagagttt aacctcaaaa
      601 atctaaccca gttacaaatg cttgcactgc tgcaaaaggt aacgaagcaa attaacgaat
      661 atcttttaca cgccccagaa gcgatgcaaa aagcgctcac tcaaaggaat cctccgccta
      721 ccttgcaact tttttccctt gcgcatgaaa aattaacaca acgtgggcta atttctcctc
      781 ttttcctatt atcgccacaa aaaaaagaat ctttcccgtt caaaaaatct tcaacaccta
      841 aagtctcgca tacgctttat ctttttggta tgcccctcct ttcggcgtat catttaacgc
      901 agcttctccg cgtttcacaa aatgtcgatg tgcatttagc agcgttacat atcccgttca
      961 taaaagaagg gaaaaatcta ttttatcaaa aagcgtcaca gcactttgag caatacacag
     1021 actttcttca aaagaaagca agcgcactta acgcaacgct gcacatcgag agtacggaaa
     1081 gcacgctttc tctgtggcaa aaagaatggg catcgggcgc ttcatcccaa acctttgttg
     1141 ataagcgtat cgaatttttg gcgttacccg ccacgtaccg aggggcagaa attatcgccg
     1201 acaaatggca tgatgctctt gtgaaagacg atactttgcg ccaagaagac ttctctctct
     1261 tacttaccga tgaaaaaaat caatttgccg cgtttgaaaa ggcatcgcac gaacgtaaat
     1321 tatctctttt tacacgcgct aaattttttg agcgcgtatc ggatgttttg gaactccttt
     1381 ctattttttc acaagcgtct tcggaaggga taacgcgtga actcgtcgta acttatctca
     1441 acaacccctt ggtgtgcgag acatttgccc tcacgcgaga gacacaagat ctttacattg
     1501 ccgcactttc aagcgcaaac ggctatcgtt ccaactacga agcctcgctc gctatttata
     1561 attttgacgc cgccataaaa cgcctccgct taggtattct cgtcgacaca caaaacgcca
     1621 cagacgaaga agtcccaggg gcagcatacc ttcgtgaatt tgacagcgta gaaatatttg
     1681 aaaatttttt gaattgcatc ggtcttcttt tagatacccc cagtgcgctc caaaaccaaa
     1741 gcgcaaaaaa aataactgaa caatttgtta aactcatcac gcgctttgaa agccccaaca
     1801 atccctttga aatcgccgag gtatgtagta ctctaaaaaa aatcgaactc gctttagagg
     1861 aacatgaaat gtcggcagcg caaattgtta gtttgctaac caacactctt tctaaaccaa
     1921 gcctcactca aaaatacgat gaattgggga tttgcgcttc ttcttttttg tctgtaacgc
     1981 cgcatcgccg gcacaccacc atttttagcc tcaacgagga aatcgataaa gacacaaaat
     2041 tagacgccga cgctttacct gagtattctc tttcccccac acggttggaa aaacgcgatc
     2101 gaactcaaat tgaacttgta caagcggcgc tttctccatg cgaagttttg c
//
LOCUS       DAOMPD010000469         2145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20122_length_2145_cov_2.679426, whole genome shotgun sequence.
ACCESSION   DAOMPD010000469 DAOMPD010000000
VERSION     DAOMPD010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20122_length_2145_cov_2.679426"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLY93_12940"
     CDS             complement(<1..853)
                     /locus_tag="PLY93_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70431.1"
                     /translation="MQILRRCRTLAFLKRGILCLVCIVTLPQAIFADADTSNLARVGI
                     LRFINNTQSENFEWVEQSLPDAIDLSMRARFEYVRLNQDQLQIAKEQVPPTENLSGGL
                     KRFSQTDVIKIAQLSNADILIYGDFIVDESSQEIVLRSVIFNAASKQIIGRVENRTPM
                     NARIFKNIDQMAAEIVSEIYRYALQENQKAGRTKDNLKLLVLVPSYTTNKEETQAKEE
                     LNVLKRELAVRTPGRYLTIFEFFDEYHVTEQEQKKSLGYAKARERVRLQLWLENYGVT
                     DAMIVLVS"
     gene            complement(912..>2145)
                     /locus_tag="PLY93_12945"
     CDS             complement(912..>2145)
                     /locus_tag="PLY93_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70432.1"
                     /translation="SQLTLSNATFTLKDWNGTVISGAPALDPDGKQFIFPASCVDAWG
                     NAPYNNPGTSAPPTNSPQVDYANGLPSGVYALTASVPSYYSFTEPSIGVTNNTVVHTG
                     YLPMVAAFKDKAVDLSDAKKIKIILTWGTGAKDLDLHLVGPSNTGYDCQAYNAPAIEN
                     TASSKFHIYFHQKYCANIGTSAPYGSANMAVDDTYEYGPEIINLNDGYVDGAYKVSVF
                     NNDTTAPNWTNSKARIYIFAGNYFSGGGGLIKMIDLHTSSTARLWRPFKFSISGTTLT
                     IDDNAGSTFGYTAVTYAQMVGSSNGLGGITDLVLSAGGLGYTVAPTVALSAPAGAGTT
                     ATATASITGNAVTGLTITSNGTGYTSTQNVTVTFSGGGGGSGAAASAVVSLFDPKTDP
                     GLFTNGTTVAGGAGLADY"
BASE COUNT          553 a          537 c          472 g          583 t
ORIGIN      
        1 cgctcaccaa cacaatcatc gcatcggtga cgccatagtt ttcaagccaa agctgcaatc
       61 gcacccgctc acgcgccttt gcatacccca atgatttttt ttgctcttgt tcggtaacgt
      121 ggtattcgtc gaaaaattca aaaatggtta aatatctccc aggagttctt accgccaact
      181 ctcttttcaa aacattaagt tcttctttcg cttgtgtttc ttctttattt gtggtatatg
      241 atggcacgag caccaaaagc tttaagttgt ctttggttcg tcccgctttt tggttttctt
      301 gtaacgcgta tcgataaatt tcagagacaa tctcagccgc catctggtcg atattcttaa
      361 aaatgcgcgc attcataggt gtgcggtttt caacgcgccc aataatttgc ttactggcgg
      421 cgttgaaaat tacactccgc agcacgattt cttggctact ttcgtccacg ataaaatcgc
      481 catagattaa aatatcggca ttcgacagtt gcgcaatttt tataacgtct gtttgcgaaa
      541 aacgtttcaa tcccccgctt aaattttctg tcggcggcac ttgttccttt gctatttgta
      601 attggtcttg atttaaacgc acgtattcaa accgcgcacg catcgataaa tcgatagcgt
      661 cgggcaaact ttgctccacc cactcgaaat tttcgctttg ggtattgttg ataaaccgca
      721 gaatacccac ccgcgccaag ttagatgtat ctgcatcggc aaaaattgct tgaggtaaag
      781 tgactataca caccagacat aagataccac gcttgagaaa agcgagagtt ctacagcggc
      841 ggagaatttg catacagtcg tttacatata atctctcaaa aacacaattc atttaattct
      901 ttctaaatca gtcaataatc cgccaatcca gcgccgcccg cgacagtggt gccgttggtg
      961 aaaagtcccg gatctgtttt gggatcaaag agagaaacga cagccgaggc agcagctcca
     1021 ctacccccac cgccaccgct aaaagtgact gtcacgttct gtgtcgaggt gtaacccgtg
     1081 ccattgctag tgattgtaag gcctgtcact gcattgccag ttatgctcgc cgttgccgtc
     1141 gccgttgtcc ctgcccctgc tggagcagaa agagcaaccg tcggcgcaac ggtgtatccc
     1201 aaaccacccg ccgacaagac gaggtcggtg ataccgccca aaccattcga cgagcccacc
     1261 atctgtgcat acgtcacagc agtgtaaccg aaggtcgatc cggcgttatc gtcgatagtg
     1321 agcgttgtac ctgatataga aaacttaaat ggccgccaca aacgagctgt gctcgaggta
     1381 tgcaaatcaa tcattttgat taagccaccg ccaccggaga aatagttccc cgcaaaaata
     1441 taaatgcgcg ctttcgagtt cgtccaattc ggcgcagtgg tgtcgttgtt aaacacagaa
     1501 actttatacg cgccgtctac ataaccgtcg ttcaaattga taatttcagg gccatattca
     1561 tacgtatcgt cgacggccat gtttgccgat ccataaggag cagatgtacc aatattcgcg
     1621 caatactttt ggtgaaaata aatatggaat ttactgctcg ccgtattttc aatcgcgggg
     1681 gcgttgtacg cttggcaatc gtagcctgtg ttagacgggc cgactaaatg taagtctaag
     1741 tcctttgcgc cggtgcccca tgttaaaatt atttttattt tcttggcatc ggatagatcg
     1801 actgctttat ctttgaaagc agccaccata ggcaagtatc cggtatgcac aaccgtgtta
     1861 tttgtgacgc caatcgaagg ttcggtaaaa gaataatacg acggcaccga agcggtgagc
     1921 gcgtacacac ccgacggcaa gccattggca taatcgactt gtggcgagtt tgtcggcggg
     1981 gcgcttgttc ctggattatt atacggtgca ttcccccatg catctacgca acttgcagga
     2041 aaaataaact gtttaccatc gggatcgagt gcgggcgctc ccgaaataac cgtgccgttc
     2101 caatctttaa gggtaaaagt cgcatttgag agcgtcaact ggctt
//
LOCUS       DAOMPD010000470         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20139_length_2144_cov_1.890857, whole genome shotgun sequence.
ACCESSION   DAOMPD010000470 DAOMPD010000000
VERSION     DAOMPD010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20139_length_2144_cov_1.890857"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PLY93_12950"
     CDS             complement(<1..1162)
                     /locus_tag="PLY93_12950"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRP70433.1"
                     /translation="MQVPEYVTNQRLIGWVEAMANLTQPDDIYFCDGSEEEYNRLCAE
                     MVTKGTLIKLNPKKRPNSYLAWSDPADVARVEERTFICSKRPADAGPTNNWMDPREMK
                     TTLNGLFNGSMQGRTMYVVPFCMGPLESPLSLYGVQITDSPYAVVSMKIMTRMGKGAL
                     QKIGDGNFIPCMHSVGVPLKKGEKDVRWPCNAENKYIVHFPEERAIWSYGSGYGGNAL
                     LGKKCLALRIASVIGRDEGWLAEHMLILGFEKPNKEKTYIAAAFPSACGKTNFAMMVP
                     PESWKGYKVTTVGDDIAWIRRAPDGGLVAINPEAGYFGVLPGTSKESNSNAMEIISKN
                     TIFTNVALTADGDVWWEGMTKEIPEGVTDWTGQPWTPDSGRHAAHPNARFTSP"
     gene            1172..1960
                     /locus_tag="PLY93_12955"
     CDS             1172..1960
                     /locus_tag="PLY93_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70434.1"
                     /translation="MQEINSACQANANFQIQADSLIYAGKPFSTTVFALNTASSQILK
                     SYKGTLNWFVIGAGTLSYSDTLAPGQSAVIALLANDASDKKIRGTSNNITAKAPVIFS
                     THRITLPAQTYMYSPFTLKVEALGSDGEIFQNYTGTALLTTLNSSGIFLQTDGITQIA
                     ELNNFVNGVSANSVEFTQPHPTLQIKLNDNADSSHFAISNLIQVAIDQLCFSALPMSN
                     ANTLRLFSNSVSNATSYYIYEKQAGNWIAFGTARPPRPRIYATH"
BASE COUNT          545 a          568 c          483 g          548 t
ORIGIN      
        1 cgggcgaagt aaaacgcgcg ttagggtgtg cagcgtgacg gccagaatcg ggcgtccacg
       61 gttggcctgt ccaatcggtg accccttcgg ggatttcttt cgtcatccct tcccaccaaa
      121 cgtcgccgtc ggcggttaag gccacgttgg taaagatggt gtttttgctg ataatttcca
      181 tcgcgttgct gttcgattct ttcgaggtgc cagggagtac gccaaaatat cctgcttcag
      241 gattgattgc cacaaggccg ccatccgggg cacggcgtat ccatgcgatg tcgtcgccga
      301 cagtggtaac tttataccct ttccacgatt caggaggaac catcatcgca aagttggttt
      361 taccgcacgc cgaagggaat gccgcagcga tgtaagtttt ttctttgttg ggtttttcaa
      421 accctaagat gagcatgtgc tcggcaagcc aaccttcatc gcggccaatg acagaggcaa
      481 tccgtaacgc gaggcatttt ttacccagga gtgcattacc gccatacccc gaaccataag
      541 accagatagc gcgttcttcg ggaaaatgga cgatatattt attttccgca ttgcacggcc
      601 agcgtacgtc tttttcgcct tttttgagag gaactcccac cgagtgcata caagggataa
      661 aattaccgtc gccaattttt tggagagccc ccttacccat acgcgtcata attttcatgc
      721 tcaccactgc atacggcgag tcggtgattt gtacaccgta gagcgaaagc gggctttcga
      781 gcgggcccat acaaaaaggc accacgtaca tcgtacgtcc ttgcatcgaa ccgttaaaaa
      841 gaccgttcaa cgtcgttttc atttcacgcg gatccatcca gttgttggtg gggcctgcat
      901 cggcggggcg ttttgagcaa atgaaggtgc gttcttcgac acgcgccacg tcggcgggat
      961 ctgaccatgc taaatagcta ttgggtcgtt ttttggggtt cagttttatc aacgtaccct
     1021 tcgtgaccat ttcggcacac aggcgattat attcctcttc gctgccgtcg cagaaataga
     1081 tatcgtcggg ctgagtcaag ttggccatcg cttcaaccca gccgataaga cgttgattcg
     1141 ttacgtattc gggtacttgc ataaaatggg aatgcaagaa ataaattcag cgtgtcaagc
     1201 taacgctaat tttcagattc aggccgattc actcatttat gccggtaagc ccttttccac
     1261 gactgtcttt gcactcaaca ccgcttcaag ccagatcctt aaatcctata aaggtacgct
     1321 caactggttt gttatcggtg cgggtacgct ttcttactcc gatactcttg ctcccgggca
     1381 aagtgcggtt atcgcacttt tggcaaacga tgccagcgac aagaaaattc gcggcacaag
     1441 caacaacatc accgcaaaag cgccggtcat tttttcaacg catcgaatta ccctacccgc
     1501 acaaacgtat atgtattcgc cttttacgct caaagtcgaa gctttgggtt cagacggtga
     1561 aatatttcag aattatacgg gcacagcact cttgacgaca ctcaacagca gcggcatatt
     1621 cttgcaaacg gatggaatca cacaaatcgc cgaactcaat aatttcgtaa atggcgtcag
     1681 cgccaattcc gtcgagttta cacaaccaca ccccacatta caaatcaaac tgaacgacaa
     1741 cgcggatagc tcgcactttg cgatttcaaa cctcatacaa gtcgctatcg atcaattgtg
     1801 tttttccgct ttaccgatga gtaatgcgaa tacgttaaga ctattttcga atagcgtttc
     1861 aaatgcaacg tcgtattata tctatgagaa gcaagccggt aactggattg ctttcggtac
     1921 tgcccgcccc ccgcgacctc gtatctatgc aactcactga cgacaggtac tgaatataaa
     1981 tatctcctcg aagcacgcga tgcaagcaat caattgtact cacttcacag actaaagcaa
     2041 cacccgctgc ctgcacgacg atatctaccg gtatgtcgaa taataacata gcgagtacga
     2101 catctcgtac atacgccgcc agcccttatt gcgtacaaga caac
//
LOCUS       DAOMPD010000471         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20301_length_2129_cov_2.665863, whole genome shotgun sequence.
ACCESSION   DAOMPD010000471 DAOMPD010000000
VERSION     DAOMPD010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20301_length_2129_cov_2.665863"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLY93_12960"
     CDS             complement(<1..526)
                     /locus_tag="PLY93_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRP70435.1"
                     /translation="MIAKIITAGRPGFHSTLITVEADILSGLPNLAIVGLPDHAVRES
                     RERIRSAITNAGFYFPARQIVINLSPNDTPKEGGLIEAAMAAAVLVASEQLPQSVFNN
                     TALLASLSLDGTLKSARALAATAVFAIAQPSIKRLIVPEDAQSEIPAPRSTEVYALSS
                     LSALRLFAADKIPRI"
     gene            659..1411
                     /locus_tag="PLY93_12965"
     CDS             659..1411
                     /locus_tag="PLY93_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRP70436.1"
                     /translation="MLKKILFSLFLVSSYAVFAQQSNAESELESAVYANDAKRIRAAV
                     KAGANPNGGNYWGHYLGIAATSGNLEAIKALVGLGADVNYATRGGWTAAMGAADNGHL
                     AVLQYLVSKKADLNAKTRMGRSILMRASYQGKTKIVQYLITKKVNINDTDFNGVTALI
                     LAAQAGHLDTVKVLLAAGADRNAETQDKRTALSIVHSILEQDAHTNGTNTQSETYKRY
                     KPIEALLKMRTMPHKIEKTKKSPKKTVRKGKK"
     gene            1422..1838
                     /locus_tag="PLY93_12970"
     CDS             1422..1838
                     /locus_tag="PLY93_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70437.1"
                     /translation="MNFIQKQKEKIKAWWLQNHTTQVFQKNVWPTLEKFIIAGPLFVG
                     FILGIWRYAPGSKQFEVAFQSLVFFVVYLSALIFFQLLDSIFFDSMPIILASIRSFLA
                     LAYIGVTLKQFFEWRHEKLQFYPLTQRLRSRISLLP"
     gene            1842..>2129
                     /locus_tag="PLY93_12975"
     CDS             1842..>2129
                     /locus_tag="PLY93_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70438.1"
                     /translation="MGDTTKEGRVIPLFSSPTHSNKKSASKQPNGSQLFRQVMGYVRK
                     RAQKRHIGWTLIPALITLRIVSTDNMKAIRAAFEKGPSNENPVPPDLLSDFL"
BASE COUNT          610 a          498 c          486 g          535 t
ORIGIN      
        1 ggatgcgcgg gattttatct gcagcaaata aacgcaacgc actcagcgaa gagagcgcgt
       61 acacctctgt ggaacgaggg gcgggtattt cactctgggc atcctcggga acaatgaggc
      121 gttttatcga cggttgtgct atggcaaaca ccgccgtcgc cgccaacgcc cgcgcacttt
      181 ttagcgttcc atcgagagag agcgaagcca aaagcgcagt gttattgaat acgctttgcg
      241 gcaactgttc tgaagcgacg agcaccgctg cagccatagc tgcctcgatg agcccgcctt
      301 ctttaggggt gtcgttcggc gatagattga tgacaatttg ccgtgcgggg aaataaaacc
      361 ctgcgttcgt aattgccgaa cggatgcgct cgcggctttc gcgcaccgca tggtcgggga
      421 gcccgacaat ggcaaggttg gggagcccgc ttaaaatgtc ggcttcgacg gtgattaaag
      481 tcgagtgaaa acccggacga ccggctgtga ttattttagc tatcatattt tatccttcta
      541 tatattaaat accaaaaaac actcgaattt tctcactttt tagaaaaaaa gtacaaattt
      601 gtgaaaaaaa atggaatttt acggttgcta ccctgtcaaa ttctagtgat tcgtacccat
      661 gttgaaaaaa attcttttct cattatttct tgtgagttcc tatgctgttt ttgctcaaca
      721 gtcgaatgcg gaatccgagc ttgaatcggc ggtatacgcc aacgacgcaa aacgtatccg
      781 tgccgctgtc aaagcggggg caaaccccaa tggcggcaac tactgggggc attatttggg
      841 aatcgccgcg acatctggta atctcgaagc aataaaagcc ttggtgggac tgggcgccga
      901 tgtcaactat gcaacacgag gcggatggac tgctgccatg ggcgctgccg ataacggcca
      961 cttagcggtg ctgcaatacc tcgtttctaa aaaagcggac ttaaacgcta agacacggat
     1021 ggggcgttcg attctcatgc gcgcgtcgta ccaagggaaa acaaaaatcg ttcagtactt
     1081 aatcactaaa aaagtaaaca tcaatgatac cgactttaac ggcgtcaccg ctcttatttt
     1141 agcggcgcaa gcagggcatc tcgatacagt aaaagtatta ttagcagcgg gggcagaccg
     1201 caacgctgaa actcaagata aacggactgc gttgagcatc gtgcattcca ttttagagca
     1261 agacgcacac acaaacggca cgaataccca aagcgaaacc tataaaagat ataaacctat
     1321 tgaggcgtta cttaaaatgc gaacaatgcc gcacaagata gaaaaaacca aaaaatcccc
     1381 caagaaaacg gtaaggaaag gaaaaaaata acaccacgcg tatgaacttt atacaaaagc
     1441 aaaaagaaaa aataaaagcg tggtggctac aaaatcacac aacacaagtc ttccaaaaaa
     1501 acgtatggcc gactcttgaa aaatttatca tcgcaggacc tctttttgtg gggtttattt
     1561 taggtatttg gcggtatgct ccgggttcaa aacaatttga agtagcgttt caatcgctcg
     1621 ttttttttgt tgtatactta agtgccctaa ttttttttca actcttggat tccattttct
     1681 tcgattcgat gcctattatt ttagcgagta tccgctcatt ccttgcgctt gcctatattg
     1741 gcgttacttt aaagcagttt ttcgagtggc ggcatgaaaa attacaattc tacccactga
     1801 cgcaacggtt acgaagtcgt atttctcttt taccataaaa tatgggcgat acaacgaagg
     1861 agggtagggt aatcccctta ttttcctccc ccactcattc caacaagaaa tccgcatcca
     1921 aacaacccaa cggctctcaa ctttttagac aagtaatggg ttatgtgcgt aaacgcgcac
     1981 aaaagcggca catcggttgg acgcttatcc ctgcgctcat cacgttgcgt attgtttcga
     2041 ccgacaacat gaaggccatt cgtgccgcgt ttgaaaaggg accgtcaaac gagaatcccg
     2101 ttcctcccga tttactgtcg gattttctt
//
LOCUS       DAOMPD010000472         2126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20331_length_2126_cov_2.409947, whole genome shotgun sequence.
ACCESSION   DAOMPD010000472 DAOMPD010000000
VERSION     DAOMPD010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20331_length_2126_cov_2.409947"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLY93_12980"
     CDS             <1..315
                     /locus_tag="PLY93_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70439.1"
                     /translation="VFEQVAKGNYLERQMGFAFASFFLSYYIGLGWAHYLIKDEGRNW
                     TKIPSDIVESIPVFFNLWKDVIVGGVQYLKPGFHPDQMNNDHLARDFFKRYEDTFAKK
                     SA"
     gene            442..1191
                     /locus_tag="PLY93_12985"
     CDS             442..1191
                     /locus_tag="PLY93_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRP70440.1"
                     /translation="MAGHSKWKNIQHRKGAADAKRGAAFTKLAKEIMVAAKLGGSDEN
                     SNPRLRAAVLKARQSSMPKDNIERAIKKGAGELEGITYEEGVYEGYGPGGIAIMVEVM
                     TDKKSRTIPELKSLFKTNGGTMADANAVAYLFDKKGLILVEGDTITEDAIMEIALDAG
                     AEDIEQDDDKVFAIKTAVNDFHTVLAAIEPKINEKNFKITESGLKFIPQTTVKVEGEK
                     AKEAIELIDALEEHDDVQNVYTNLEWSDNLV"
     gene            1227..>2126
                     /locus_tag="PLY93_12990"
     CDS             1227..>2126
                     /locus_tag="PLY93_12990"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRP70441.1"
                     /translation="MPQKLKTMKKIPMLRMLSSVFFLISLIPFSLWAAPTVLVDTVAI
                     VANEPVTSVDLANEIQFMKKRKGFKKEKRGLESQALDILIGRAIISSIAREEAVTISA
                     ERVETQIQKEMDMRGILRQAEFEKIIKRDTGLTLDEYKEEMAHQLLTQQVMQLRVSVP
                     NPTPSQVEEWYRHNKARVGKKYNFRMIYIPVHGNEVQVNKQMKAARAMAGNSAVSFAK
                     AAAKYSAHSSKSRGGLLVDMRLDQIAQIDPILAGAVNGSPVGSVSQVFVGSNGAYYCI
                     RVESARAIGLEEIYDNVRAILYGQ"
BASE COUNT          629 a          484 c          519 g          494 t
ORIGIN      
        1 gttttcgagc aagtcgccaa agggaactac ctcgagcggc aaatgggttt tgcttttgct
       61 tcatttttct tgtcgtatta tatcggcctc ggctgggcgc actatctcat caaggacgaa
      121 ggccgcaact ggacaaaaat tccttcggac attgttgagt cgattccggt gtttttcaac
      181 ctttggaaag acgtcattgt cggtggggtt cagtatttga agccaggttt ccaccctgac
      241 cagatgaaca acgaccactt ggcgcgcgat ttcttcaaac gttacgaaga tacgttcgca
      301 aagaaatcgg cataacccgt aaatagttgg agctgtcgaa taagctgctg gtgcggctat
      361 tcgacagcct cgggctaatt gattgtattt tttttgaatc ctgctttttg cgacatctaa
      421 tatcccacgt ttgtcctttc tatggcaggc cacagtaaat ggaaaaatat acagcaccgt
      481 aaaggtgcag ccgacgcgaa acgcggtgca gcttttacaa agctcgcaaa agaaattatg
      541 gttgcagcga agttgggcgg cagcgatgaa aactcaaacc cacgcttacg ggcggcagtg
      601 ttgaaagcac gtcaaagctc gatgcccaaa gacaacatcg aacgggcaat caaaaaaggc
      661 gcgggcgaac tcgaaggcat tacctacgaa gagggggttt atgaaggtta tggcccaggt
      721 gggattgcta ttatggtcga agtgatgacc gataaaaaat cacgcacaat acccgaactc
      781 aaaagccttt ttaagacaaa cggcggcacc atggccgacg ccaacgcggt cgcttatctc
      841 ttcgataaaa aaggtctcat cctcgtcgaa ggcgacacca ttaccgagga cgccatcatg
      901 gaaattgcac tcgatgcagg cgcggaagat atcgaacaag acgacgacaa agtttttgcg
      961 ataaaaactg ctgtgaatga tttccacacg gtgcttgctg cgattgagcc caagatcaac
     1021 gaaaagaatt tcaagattac cgaatcgggt cttaaattta taccacaaac cacagtcaaa
     1081 gtcgaaggcg aaaaagcaaa agaagcaata gagttaattg atgcgctcga agaacacgac
     1141 gacgtacaaa acgtctacac caatttggag tggagcgaca atctcgtttg acggcaggcg
     1201 tcgcgaacat ttactttccc gtgcaaatgc cacaaaaact taaaacaatg aaaaaaatac
     1261 caatgcttcg aatgctttcg tcggtatttt ttttaatcag tcttattcct ttttcactct
     1321 gggcagctcc cactgttctt gtggacacag ttgcgattgt cgcaaatgag ccggtgacca
     1381 gcgttgactt agctaatgaa attcagttta tgaaaaagcg caaaggcttc aaaaaagaaa
     1441 agcgtggttt ggaaagtcag gctctcgata tacttattgg tcgcgctatt attagcagca
     1501 tcgcgcgcga ggaagcggtc acgatttcag ccgaacgcgt ggaaacacaa atccaaaaag
     1561 agatggatat gcgcggtatt ttacgtcaag cagagttcga aaaaataatt aaacgcgaca
     1621 ccggtttaac cctcgacgaa tataaagaag aaatggcgca tcaacttttg acgcaacaag
     1681 tgatgcaatt acgcgtgtcg gtgcccaacc ccacaccgtc gcaagtggaa gagtggtatc
     1741 gacacaacaa agcgcgcgtg ggaaaaaaat ataactttcg tatgatttac attccggtgc
     1801 atggcaacga agtgcaagtg aataagcaaa tgaaagctgc gcgcgcgatg gcgggaaact
     1861 ctgccgttag ctttgcaaaa gcggcggcaa aatactctgc acactcgtca aaatcaagag
     1921 gcggactact cgtcgacatg cgcctcgatc aaattgcaca aatcgatccc attttagcgg
     1981 gtgcggtcaa cggttcgcct gtcggctcgg tgtcgcaggt gtttgtcggt tcaaatggag
     2041 catactactg cattcgcgtt gaaagcgcgc gcgctatcgg tctcgaagaa atatacgaca
     2101 acgtgcgggc aattttgtac ggccaa
//
LOCUS       DAOMPD010000473         2119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20421_length_2119_cov_2.987888, whole genome shotgun sequence.
ACCESSION   DAOMPD010000473 DAOMPD010000000
VERSION     DAOMPD010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20421_length_2119_cov_2.987888"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLY93_12995"
     CDS             <1..1307
                     /locus_tag="PLY93_12995"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRP70442.1"
                     /translation="QDSIRQGFKSLPNGMKDSGVEWLGMVPAHWEVKRLKYIARFRSG
                     ESITSENIEPEGQFPVYGGNGQRGFASNYTHDGEFILIGRQGALCGNINFANGKFWAS
                     EHAVVVRSTAKIVVRWLGELLYAMNLNQYNISAAQPGLAVERIQELAIPLPPLSEQRA
                     IAAYLDRETARIDTLIAKQEQLIALIDEKRQALISHAVTKGVSHSIRQGFKSLPNGMK
                     DSGVEWLGAIPEHWEVKRLKYVLNLVTRKVPVGSSKAIALENIQSWTGNIVESETEYE
                     SEGIEFHAGDILFGKLRPYLAKVYRAESNGISVGDIFVLRAGNQNSTHFYSLILRTRD
                     YISAIDSSTYGAKMPRVSWEYFSNLPIPLPPLDEQRAIAAYLDRETAKLDTLKAKAQS
                     AIELLREKRSALISAAVTGKIDIRPDFIRQGFKSLPNENRGE"
     gene            1311..1820
                     /gene="tnpA"
                     /locus_tag="PLY93_13000"
     CDS             1311..1820
                     /gene="tnpA"
                     /locus_tag="PLY93_13000"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRP70443.1"
                     /translation="MLKKGNSYYELYYHVVFATKRREAMLSLAKISEIREIVKRKSDE
                     MGFLSHIVNGYVDHIHMLVTIPPSLAIATVVKHIKGVSSRSIDELYWQEGYWVQGIEK
                     KSVSTVAAYIENQWHHHQTFIRQGFKSLPNENDLPNEINLIEMDYLPNENSRNDHGVE
                     TPVLSGVNP"
     gene            1817..>2119
                     /locus_tag="PLY93_13005"
     CDS             1817..>2119
                     /locus_tag="PLY93_13005"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HRP70444.1"
                     /translation="MKLERFSAGKWQQRLQYKSFEPIMVNHEWTWEDARINILLEQAN
                     RALGELNAFSLIVPDIDLFIQMHVLKEAQMSSRIEGTQTGIDEVLMPEEDISIEKRD"
BASE COUNT          611 a          465 c          533 g          510 t
ORIGIN      
        1 ggcaggattc cattaggcag ggatttaaat ccctgcctaa tggaatgaaa gattccggcg
       61 tcgaatggct gggtatggtg ccggcgcatt gggaggtgaa gcgattgaag tatattgcac
      121 gatttcggag tggcgagagt ataacatctg aaaatatcga accagaaggt cagtttcctg
      181 tatatggcgg caatgggcaa agaggctttg caagtaatta tactcatgat ggtgaattta
      241 ttctaattgg ccgacaaggt gcgctctgtg gtaatatcaa ctttgcaaat ggtaagtttt
      301 gggcgtcaga acacgcggtt gtcgttcggt caactgcaaa aattgttgtt cgctggcttg
      361 gcgagctgtt gtacgcaatg aatttgaatc agtacaatat ttcagcggcg caacctggtt
      421 tagcagttga gcgtattcaa gaactcgcca tccccctccc acccctttcc gaacaacgcg
      481 ccatcgctgc ctacctcgac cgcgagaccg cccgcatcga taccctcatc gccaagcaag
      541 agcagctgat tgcgctcatc gacgaaaaac ggcaggcact catttcgcat gcggtcacga
      601 agggggtttc gcattccatt aggcagggat ttaaatccct gcctaatgga atgaaagatt
      661 ccggggttga atggctgggc gcaataccgg agcattggga ggtgaagagg ctgaaatatg
      721 tactgaattt ggttacaaga aaagtacctg ttggctcatc aaaggcgata gcacttgaga
      781 atattcagag ctggactggc aatatagttg aatccgaaac agaatatgaa tctgaaggca
      841 tagaattcca tgctggggat atcctatttg gcaagttgcg accatactta gcaaaggtct
      901 atcgggcaga aagtaacggc atttcggtcg gcgatatctt tgtcctgcga gcaggtaatc
      961 aaaacagtac acacttctat agtttgatat tgcgtacccg agattatatt tcggcaattg
     1021 atagctctac ttatggcgcc aaaatgcccc gcgtaagttg ggaatatttt tcaaacctcc
     1081 ccatccccct cccgcccctc gacgaacaac gcgccatcgc tgcctacctc gaccgcgaga
     1141 cggccaagct cgacacgttg aaggccaaag cgcaatcggc gattgagctt ctgcgcgaaa
     1201 aacgctccgc cctcatctcc gcagccgtca cggggaagat tgacattaga ccggatttca
     1261 ttaggcaggg atttaaatcc ctgcctaatg aaaatagagg cgaatagaat atgctaaaaa
     1321 agggtaatag ctattacgag ctatattatc acgtcgtttt tgcgacgaag aggcgcgagg
     1381 cgatgttgtc gttggcaaaa attagtgaaa ttagagaaat cgtcaaacgc aagagcgacg
     1441 aaatggggtt cttgagtcat attgtgaatg gttatgtcga tcatatacac atgctcgtaa
     1501 cgataccacc ttcgctggct attgcaacgg tggtgaaaca tataaaggga gtttcatcac
     1561 gtagtataga tgaactttat tggcaagaag ggtattgggt tcagggcatc gaaaagaaaa
     1621 gtgtgtccac ggtagcagct tatattgaaa accaatggca tcatcaccag actttcatta
     1681 ggcagggatt taaatccctg cctaatgaaa acgatttgcc taatgaaatc aatctaatag
     1741 aaatggatta tctgcctaat gaaaacagtc gaaacgacca tggagtcgaa acacctgtgc
     1801 tgagcggagt caacccatga aactcgagag attctccgca ggcaaatggc aacaacgcct
     1861 gcaatataaa agctttgagc ccatcatggt gaaccatgaa tggacgtggg aagacgcgcg
     1921 aatcaacata ctgcttgagc aggccaaccg ggctctcggc gaattaaatg cattttctct
     1981 catcgttcca gacattgacc tgtttattca gatgcacgtt ttgaaagagg cgcagatgtc
     2041 gagccgcatt gaaggaacgc agaccggcat cgatgaagtg ctgatgcccg aagaagatat
     2101 tagtatcgaa aaacgcgat
//
LOCUS       DAOMPD010000474         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20437_length_2118_cov_2.260301, whole genome shotgun sequence.
ACCESSION   DAOMPD010000474 DAOMPD010000000
VERSION     DAOMPD010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20437_length_2118_cov_2.260301"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLY93_13010"
     CDS             complement(<1..400)
                     /locus_tag="PLY93_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70445.1"
                     /translation="MEITGSSISIPRNFLFLHKLPARFEMLVIARGAAFKESTAYEVE
                     LLGQRLVLRSKVELKPGTKYELEKENALVFRIVKEKHSENDNISDRHIASETIKEAYS
                     DFENLSFQSFYGMTNLDLLSLKAIEEEGYSV"
     gene            complement(421..1554)
                     /locus_tag="PLY93_13015"
     CDS             complement(421..1554)
                     /locus_tag="PLY93_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRP70446.1"
                     /translation="MFKKMNTLFLFAIFLLPLSAILSAVTNEEITKLVDACSEPVASG
                     YAKVLPRFSKVLPEKKADPLKTLEAVVPRACYLELQEWQLQTALQLGHYGLNQGLLPS
                     TISDLTEILSWHTVAKEVYVKLGRVYEKMRQAGAAPDEIAQVFYTAQQKNLSPDQSEV
                     FALRYSEYRVAGKKHNEAFHSAQDEIQEIKKLHGKKIKSYVFEKDQSPILHTLQEGSP
                     EQDTLWEKLRGQIQEEKSFGLVIPQKSQKNWNFEKLEDFFEMWKGTPYKWGGFNKKGI
                     DCSGFVIKAIESQFPQSKMPRSASQLAKFGSDVARDKLTTGDLVFFAASDVPGRITHV
                     GIYLADGKFAHASSKRGVTLANIGDKYYVERFVSAKRLFDTAQ"
     gene            complement(1558..>2118)
                     /locus_tag="PLY93_13020"
     CDS             complement(1558..>2118)
                     /locus_tag="PLY93_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRP70447.1"
                     /translation="RVTENNEVSRSDAGVKEKDVGSSVQDGGILLKKSAVPASRLAEI
                     AFKPFAAQFAERNISVSLNLPHTLPYLVCDIAKVAWVLSIFLSNAVRYTPEWGKLNVS
                     ASLLNAKLRIAVENSGYGISIESAERMFTRVENESAIEFGQGLALILAREIIEAHGGL
                     IGVESELGVMTRFYFEIPIENTTLRT"
BASE COUNT          561 a          524 c          400 g          633 t
ORIGIN      
        1 taacgctata cccttcttcc tcaatagcct ttaacgacaa taaatcgaga tttgtcattc
       61 cataaaaact ttgaaaagat aaattttcaa aatcgctata ggcttcttta attgtttccg
      121 acgcaatatg cctgtcgcta atattgtcgt tctcgctgtg cttttctttc actatgcgaa
      181 agacaagcgc attttccttt tccagttcgt attttgtccc aggctttagt tccactttcg
      241 agcgtaaaac taatctttgc cctaaaagct ccacctcgta cgcggtgctt tctttgaaag
      301 cggcgccgcg tgcaatcacg agcatttcaa aacgcgccgg tagtttgtgt agaaaaagaa
      361 aattacgtgg tatcgatatc gacgaaccgg taatttccat cgaaccgttg acgctaaaag
      421 ttattgcgcc gtgtcgaaca gacgtttggc cgaaacaaac cgctcgacat agtatttatc
      481 gccgatgttc gcaagcgtca caccgcgctt tgaagaggcg tgcgcaaatt ttccatccgc
      541 taaataaatt ccaacgtgcg taattctacc cggaacatcc gaggcggcaa agaagactaa
      601 gtcgcctgta gttagtttat cgcgtgcaac gtcgctccca aattttgcta attggctggc
      661 gctgcgcggc attttgcttt gtggaaattg cgattcaatc gctttaatca caaagccaga
      721 gcaatcgatc ccttttttat taaacccacc ccatttatac ggagtgcctt tccacatctc
      781 aaaaaaatct tctaattttt caaaattcca gtttttttga gatttttgtg ggataactaa
      841 tccaaaactt ttttcttctt ggatttgacc gcgcagtttc tcccataacg tatcctgctc
      901 cggtgaccct tcttggagtg tgtggagtat cgggctttga tctttttcaa agacgtagct
      961 ttttattttc ttgccatgca attttttaat ttcttgaatt tcatcttgag cgctgtggaa
     1021 cgcttcgttg tgttttttac ccgcaactcg gtactccgaa tagcgcagtg caaaaacctc
     1081 gctttgatcg ggagaaagat ttttttgttg cgcggtataa aaaacttgcg caatttcatc
     1141 gggcgccgcc cccgcttgtc tcattttctc gtagacccgc cccaacttca catacacttc
     1201 tttagcaacg gtatgccaac ttaaaatttc tgttaaatcg gaaatcgtcg atggaagtaa
     1261 tccttggttc aagccataat gccccaactg caacgccgtt tgcaactgcc attcttgcaa
     1321 ttctaagtaa cacgcgcgcg ggaccaccgc ctctaaggtc tttaatggat cggccttttt
     1381 ctcaggtaaa actttactaa agcggggtag cacctttgca taaccgcttg ccaccggttc
     1441 ggaacaagca tcgaccaact tagtaatttc ttcattcgtc acagcgctta aaattgccga
     1501 caatggcagc aaaaagatag caaataaaaa tagagtattc attttcttaa acatacttca
     1561 tgtccttaaa gttgtatttt caatgggaat ttcaaaataa aaacgcgtca ttactcccaa
     1621 ctccgactca acgccaatga ggccgccgtg tgcctcaata atttcacgcg ctaaaataag
     1681 cgccaaacct tggccaaact ctattgcgct ttcattttca acgcgcgtga acatccgctc
     1741 cgcactttcg atagaaattc cataccctga attctcgaca gcaatccgca attttgcatt
     1801 gagcaacgac gcagacacat tgagctttcc ccattcgggg gtgtaacgca ctgcattact
     1861 taaaaaaatt gaaagcaccc aagcgacttt agcaatatcg caaactaaat atggtaaagt
     1921 atgcggtaaa tttaagctta ctgaaatatt tctttcggca aactgagccg caaaaggttt
     1981 aaatgcaatt tctgccaagc gcgatgcagg caccgcgctt tttttaagga ggataccccc
     2041 gtcttgcacg ctggaaccga cgtctttttc tttaacgcct gcgtcgctcc gagagacttc
     2101 gttattttcc gttactcg
//
LOCUS       DAOMPD010000475         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20475_length_2115_cov_2.806796, whole genome shotgun sequence.
ACCESSION   DAOMPD010000475 DAOMPD010000000
VERSION     DAOMPD010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20475_length_2115_cov_2.806796"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLY93_13025"
     CDS             <1..287
                     /locus_tag="PLY93_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRP70448.1"
                     /translation="GELFVGAQTPVYVGMIIGLHAKDNDLDVNPIREKKLTNVRASGT
                     DEALRLVPPRKLSLEQCLDFLEDDEALEVTPTSLRLRKKVLNPSQRKRSA"
     gene            295..1149
                     /locus_tag="PLY93_13030"
     CDS             295..1149
                     /locus_tag="PLY93_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRP70449.1"
                     /translation="MVDFFSPANLKSLWVTLSTGHAFAATGIVIALLLTIRAIILRIA
                     NRSIQDSFMRMRYRRGVSYAMFFVGIFLLLPIWLPSIRNIATFLGIFGAGFLIVTKDL
                     WICVGGWAYIMIRRPYAIGDRVEVGEIAGDVIDIRLMETSIMEVSNTEGRLTTGRIIY
                     FPNAKVFTEIIATTSKEIAHTYQEMHVRLGPASDWQAVEKILIKATYEEYKFAVEEKN
                     KLENHEVRYTVSGYREPRVLTEMQDGHIVLHLQFMAPHGLGTIMTDRIWRSFLSQVQK
                     DPTIQFGS"
     gene            complement(1134..>2115)
                     /locus_tag="PLY93_13035"
     CDS             complement(1134..>2115)
                     /locus_tag="PLY93_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70450.1"
                     /translation="DAVRYLKEAIRLAGKANEREAKTLTILAQKLSLKRLDNKNGLGD
                     NNTADIRVDGDDVYIATWGAGLYRYTRSTDTMKRIQLPSPQLRGLYVDFDEIFITSFD
                     GVYRLSKKDGTVASLKDESGELKLGQKTIKDDRYIYFTTLTRGLVQYDTIKKKIQTFG
                     KNSWVGSNQVYALDADLTYIVVGTIDHGVKIHNKLTGETVSLGVEENGLRSANIKAAL
                     IDGRYVYIGAHDDGVYVYDMKEKKLSRLPVDAPFPSALAKRENEILIGTSGEGVRIFS
                     RNNNRVEKLRAIEGLSSNEVQIIRVEGDFLWIGYLEKGIDVFFLPSHEPN"
BASE COUNT          541 a          515 c          502 g          557 t
ORIGIN      
        1 gaggagagct ttttgtaggt gcgcagaccc ccgtgtacgt tggcatgatt atcggcttgc
       61 acgccaaaga taacgacctc gatgtaaatc ctatccgtga aaaaaagctc acaaacgtgc
      121 gtgcgtcggg taccgacgag gcactgcgct tagtgccgcc acgtaaactt tccttggagc
      181 aatgcctcga ttttttggaa gacgacgaag cgttagaagt caccccgacc agtttacgct
      241 tacgtaaaaa agtactcaat ccttcgcaaa gaaaacgcag cgcataaaga aagaatggtt
      301 gatttcttct cacctgcaaa tttaaaaagt ctgtgggtga cgctttcaac cggtcatgcg
      361 ttcgccgcca ccggtattgt cattgcactc ctgctgacga tccgcgcaat tattcttcgc
      421 atcgccaatc gaagcattca agatagtttt atgcggatgc gttatcggcg cggcgtttcc
      481 tatgcaatgt tttttgtggg catttttttg ctcctgccaa tttggctgcc gtcaatccgc
      541 aacattgcaa cctttttggg gattttcggc gccggttttt taatcgtcac taaagattta
      601 tggatctgcg ttggcggttg ggcgtacatt atgatccgtc gcccatacgc catcggcgac
      661 agggttgaag tgggtgaaat tgcgggtgac gtcatcgaca tccgcctcat ggaaaccagc
      721 attatggaag tttccaacac cgaaggacga ttaaccacgg ggcgcattat ttatttcccg
      781 aatgcaaaag tttttaccga aattatcgcc accaccagca aagaaatcgc acacacctat
      841 caagaaatgc acgtccgttt aggtccagca tcggattggc aggcggtaga aaaaatttta
      901 atcaaagcaa cttacgaaga atacaaattc gccgtagaag aaaaaaataa attagaaaat
      961 cacgaagtgc gctacaccgt ttcggggtac cgcgaaccgc gcgtgctgac agaaatgcaa
     1021 gacggccata tcgtattgca tttgcaattt atggcgccgc atggtttggg taccattatg
     1081 acagaccgca tctggcgttc ttttttatcg caagtgcaaa aagaccccac gattcaattt
     1141 ggttcgtgag acggcagaaa aaacacatcg attccctttt ccaaataacc aatccaaagg
     1201 aaatctcctt cgacacgaat aatttgtact tcgttcgagg ataatccctc gatggcgcgc
     1261 aacttctcga cgcggttatt gttgcggcta aaaatgcgca ctccctcgcc cgaggtgccg
     1321 atgagaattt cgttttcgcg cttcgccaac gccgacggga aaggcgcatc gacaggtaga
     1381 cgactgagtt ttttttcttt catgtcgtaa acataaacgc cgtcgtcgtg cgcgccaata
     1441 tagacgtacc ggccatcgat gagcgccgct ttgatgttgg cagagcgaag accgttctct
     1501 tcgacgccga gagaaaccgt ctcgcctgtt aacttgttgt gaattttgac gccgtggtcg
     1561 atggtgccaa cgacgatata cgtgagatcg gcatcgagtg cataaacttg gtttgagccc
     1621 acccacgaat ttttgccaaa ggtttgaatt ttctttttga tagtgtcgta ttgcacaaga
     1681 ccgcgtgtga gtgtggtgaa ataaatataa cgatcgtcct tgatcgtttt ttgccccagc
     1741 ttcagctcgc cactttcgtc cttgagcgag gcgacagtac cgtccttttt tgagagacga
     1801 taaacaccgt cgaaagaagt aataaaaatt tcatcgaaat caacatagag accgcgtaat
     1861 tgtggcgacg gtagttgaat gcgcttcatt gtgtcggtag agcgggtgta tcgataaagc
     1921 cccgcccccc acgtcgcaat ataaacatcg tcgccatcaa cgcggatgtc ggcggtgtta
     1981 ttgtcgccca aaccattttt gttgtcgagc cgcttcaacg acaatttttg cgccaaaatt
     2041 gtaagcgtct tagcctcgcg ttcgttcgct ttaccagcaa ggcggattgc ttcttttaag
     2101 taacgcactg cgtct
//
LOCUS       DAOMPD010000476         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20499_length_2113_cov_1.970360, whole genome shotgun sequence.
ACCESSION   DAOMPD010000476 DAOMPD010000000
VERSION     DAOMPD010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20499_length_2113_cov_1.970360"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLY93_13040"
     CDS             <1..883
                     /locus_tag="PLY93_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP70451.1"
                     /translation="PKEKRGYGTMLVAGFGILGAVVATLIGDLFKWQTAYIIGSVMGL
                     LLLILRISVYESGMYEKTAETGVKRGNFLYLFTDSERFYLFTSRERFMRYFRSILIGV
                     PLWFVVGILVIFSKEFGQALGANFTINPGHAIGFMYAGLSVGDFLTGYISQRIGSRRK
                     VVLVCQFLNVGALALYLFSSNFGPIYFYTLCVFLGLINGYWAVFITIGAEQFGTNLRS
                     TVATTVPNFVRGSVPLLTTAFLFFDQEVFGKGLVNSAQGMVPAAFIVGGGSLLLGIWA
                     TLTIDETHGKDLNYVEQ"
     gene            917..2113
                     /gene="tyrS"
                     /locus_tag="PLY93_13045"
                     /pseudo
     CDS             917..2113
                     /gene="tyrS"
                     /locus_tag="PLY93_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
BASE COUNT          535 a          466 c          543 g          569 t
ORIGIN      
        1 gccgaaagaa aagcgtgggt acggtaccat gctggttgcc ggttttggaa tcttgggagc
       61 ggtggttgca acgctcatcg gtgatttatt caaatggcaa actgcgtaca tcatcggtag
      121 tgtgatgggg cttctccttt tgattttgcg catcagtgtt tatgaatcgg ggatgtacga
      181 aaaaacggcg gagacgggcg tcaaacgcgg caactttctt taccttttca ccgacagcga
      241 acgtttctat cttttcacgt cacgcgagcg ctttatgcgt tattttcgat cgattctcat
      301 tggtgtgccg ctgtggtttg tggtgggtat ccttgtgatt ttctccaaag aatttgggca
      361 ggcattgggg gcgaatttca ctatcaatcc cgggcatgcc atcggcttta tgtacgccgg
      421 tctttccgtg ggggatttcc tcacggggta tatcagccaa cgcattggct cgcgtcgaaa
      481 agtggttctt gtatgtcaat ttttgaatgt gggcgctctc gcgctttatc ttttctcttc
      541 taattttggt cccatctatt tttacacgct gtgcgttttt ttagggctca tcaacggtta
      601 ttgggctgtt tttatcacca tcggggcaga gcagtttggc accaatttac gttcgaccgt
      661 cgccaccact gtccccaatt ttgtacgcgg ttcggtgccg ttactcacca ccgccttctt
      721 attttttgat caagaagttt ttggtaaagg tttggtcaat agtgcgcaag gcatggtgcc
      781 cgcagcgttt atcgtcggcg gtggttcact tcttttgggg atatgggcga cgcttacaat
      841 cgacgaaacg cacggcaaag acttgaacta tgtggaacaa tagttcccac cgcgtctcaa
      901 aatctgagtt tagcgtgtgg ccgaaaagca agagaaaatt gagcttccgc gtgaagtaga
      961 aaacgagttt gaagaactca cccgtggcat cgaagaagta ttaccggtgc atgagtttcg
     1021 caagaaatta ttaaacgcgt acaacacaaa aacaccgctc aaagttaaag cgggattcga
     1081 tcccaccgcg cccgacttac acttggggca cacggtgctc atccaaaaat tgcggcagtt
     1141 ccagaaattc ggccacgaga ttcttttttt aatcggcgat tatactgcaa cgattggcga
     1201 tcccaccggg aaatcagaaa cgcgcaaagt tctctcgcgc agcgcagtgg aagaaaatgc
     1261 aaaaacatac aaagagcaag tttttaaaat tttagatccc cagaaaacaa aagtagtatt
     1321 taactctacg tggttggatt cttgaaagct tgaggattta ctttatttaa ccggtaaata
     1381 caccgtggcg cgaatgttgg agcgcgatga tttttcaaag cggtacgctg acggccaacc
     1441 gatttcattg gtggagttta tgtacccgct catgcagggg tacgattcgg ttgcgctcaa
     1501 gagcgacatt gaattgggag gtaccgacca aaaatttaat ttactcgtcg ggcgtgactt
     1561 acaagcagct tatggtcaaa gtggccagtg cgtgatgatg acaccgcttt tggtagggct
     1621 cgacggtata aaaaaaatgt cgaagtcgtt ggggaactac atcggtatcc aagagctacc
     1681 ggcggatatt tacggcaagc tcatgagtat ttccgatagc ttgatgctcg attattatac
     1741 attgctttcc gataaaacaa cgacagagct tgttcgtctt aaagatgcgc ttaaaaacgg
     1801 ttcggtgcat cccaaagaag caaaggcagc gctggcgttt gaaattactc accgcttcac
     1861 ttccgaagag actgcccgca caactgccgc cgagtgggag aaagtccata accctaaaga
     1921 acgcggtttg ccgcaggaaa tccccgagtt ccattacacc aaggggacgc agcttgtgag
     1981 tattgttaaa gaagcaggac ttgccgcgtc gaactcggaa gcgcggcgga tgattgagca
     2041 aaagtcgatt tatcgtgtgg acgaaaatgg tctcgaagaa gcgttggtgg atttcaaaat
     2101 gcccgtgaac gaa
//
LOCUS       DAOMPD010000477         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20555_length_2108_cov_3.132002, whole genome shotgun sequence.
ACCESSION   DAOMPD010000477 DAOMPD010000000
VERSION     DAOMPD010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20555_length_2108_cov_3.132002"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLY93_13050"
     CDS             complement(<1..572)
                     /locus_tag="PLY93_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP70452.1"
                     /translation="MTDQTLPEKKKILIVEDEDVQLIALETFLSQWGFTIIAVKTATA
                     AIAWLSQNEPDMALIDLHLPDNPGIEILKHIRDTDGSIPVPVFTMSAEDSPEIRIVCM
                     STGADDFLPKPLNFADVSMKIQQAFERVSYKKEIQKLNAKLEKDKKSLQRYFSADLVE
                     EILNEEFSTELGGQIVSASIMFFDIRGSTT"
     gene            complement(594..1421)
                     /locus_tag="PLY93_13055"
     CDS             complement(594..1421)
                     /locus_tag="PLY93_13055"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRP70453.1"
                     /translation="MSSIIIPPLPTIVAQVIQFDANNPKNGAADLEKLVIPDKAISAE
                     LLRVANSAFYGRSGKVKILRDALAVLGIKATKNLVIYLSTKTMASTYKSEAFKKYLNR
                     FPIYSALIAQGIAQETSIRAQADEAFLAALLHSIGMNIMALQNQGHFSDMLQACEKNK
                     WDLLKLEKQSYGTTHIELGKKAALEWKLPDEFQPLMEISPSSDCASLVSPIEKVTFVA
                     SVCASLLLGIPVTETAKTGAQRCYNELGGTHNVLLRHAAPDTQVKIEAHPFTQLASV"
     gene            complement(1425..1832)
                     /locus_tag="PLY93_13060"
     CDS             complement(1425..1832)
                     /locus_tag="PLY93_13060"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRP70454.1"
                     /translation="MPEMEWSNDYATGIDEIDKQHIFLFDLVNRLIREAAISATPASI
                     NEVIEELVRYTVYHFDFEEGIMKKVNYPHYTEHKKKHEGLKAQVQLYAAELRAGGLDM
                     VDFVDFMKSWLKFHILREDMKYVAALAPVWHGK"
     gene            complement(1847..>2108)
                     /locus_tag="PLY93_13065"
     CDS             complement(1847..>2108)
                     /locus_tag="PLY93_13065"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRP70455.1"
                     /translation="AGVFIFGMITRVALGHTGRPIKTAPMITFAYLTLNLALLVRVVV
                     PLTGAAQMGYLIAGTLWLISFILYLVKYTPMLVRPRIDGRPG"
BASE COUNT          536 a          486 c          480 g          606 t
ORIGIN      
        1 atggtggttg aaccacggat atcaaaaaac ataatcgacg cagatacaat ctgcccgccc
       61 aattcggtag aaaactcttc gttcaaaatt tcttcaacga ggtcggcaga aaaataccgc
      121 tgcaaactct ttttatcctt ttctaatttt gcgttaagtt tctgaatttc ctttttgtaa
      181 ctcacgcgct cgaacgcttg ctgtatcttc atcgacacat cggcaaaatt gagcggctta
      241 ggtaaaaaat cgtctgctcc cgtagacatg catacaatgc gaatttcggg agaatcttcg
      301 gcggacattg taaagactgg cacagggatt gaaccgtcgg tatcgcggat gtgtttcaaa
      361 atttcaatcc ccggattgtc tggcaagtgt aagtcgatga gcgccatatc cggttcattt
      421 tgactgagcc atgcaatcgc tgcggttgct gttttaacag caatgatagt aaaaccccat
      481 tggctaagaa atgtttccaa cgcgatgagt tgcacgtctt cgtcttctac aatgaggatt
      541 ttcttttttt caggaagcgt ttgatcggtc atagaaagtc atcgtttttt tacttaaacc
      601 gacgcaagtt gtgtaaaagg gtgtgcctct atttttactt gtgtgtcggg cgcagcgtgg
      661 cgcaaaagca cgttatgcgt gccacctaac tcattgtaac atcgctgtgc gcctgttttg
      721 gctgtttcgg tgacaggaat acctaacaac aacgaggcac agaccgacgc aacgaacgtc
      781 actttttcaa tgggtgaaac aagtgacgca caatcgctgg agggtgagat ttccataagg
      841 ggttggaatt catcgggtaa cttccattct aacgccgctt ttttgccaag ctcgatatgc
      901 gtcgtaccat aagattgctt ttctaatttg agtaaatccc acttgttttt ttcgcacgct
      961 tggagcatgt cgctgaagtg cccctgattt tgtaatgcca taatattcat accgatgcta
     1021 tgtagcaacg cggctaaaaa tgcttcatcg gcttgcgctc ttattgaggt ctcctgcgcg
     1081 atgccttgtg caatgagagc agaataaata gggaaacggt tgagatattt tttaaatgcc
     1141 tcgcttttgt aagttgaagc cattgttttg gtgcttaaat aaatcaccaa attcttggtc
     1201 gctttgatgc ccaacaccgc gagagcatcg cgcaatattt ttactttccc cgagcgacca
     1261 taaaaggcag agtttgccac gcgcaagagt tctgcgctaa tcgctttatc gggtatgaca
     1321 agtttttcta aatcggcggc gccattcttg gggttgttgg cgtcaaattg gattacctgc
     1381 gctacgattg tgggtagtgg cggtatgatg attgaactca ttttttactt cccatgccat
     1441 acaggggcca acgctgcaac gtacttcatg tcttcgcgta aaatgtgaaa tttaagccac
     1501 gatttcatga aatcgacaaa atcgaccatg tctaacccac ctgctctaag ttcggcagcg
     1561 tagagttgca cctgtgcttt taggccctcg tgtttttttt tgtgctccgt gtaatgtgga
     1621 tagttcactt ttttcatgat gccttcttca aagtcaaaat gatacactgt atagcgcacg
     1681 agctcttcga tgacttcatt gatgctcgct ggtgttgccg agatggcggc ttcacgaatg
     1741 aggcgattga ctaaatcaaa aagaaaaata tgttgcttat cgatttcgtc gattccagtt
     1801 gcgtaatcgt tgctccactc catctctggc atatttcttc acaacgctac ccgggtcttc
     1861 catcaatacg ggggcgaacc agcatcggcg tgtacttcac aaggtacaga ataaaactga
     1921 tgagccacag cgtgcccgcg attaaatacc ccatttgcgc tgcgccggta agcggcacaa
     1981 ccacgcgcac caaaagcgcc aagttgagag ttaagtaagc aaacgtaatc atcggcgccg
     2041 ttttgatggg gcggccggtg tgccccaatg ccacgcgggt aatcatacca aaaataaaaa
     2101 cacccgcc
//
LOCUS       DAOMPD010000478         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20730_length_2095_cov_2.511765, whole genome shotgun sequence.
ACCESSION   DAOMPD010000478 DAOMPD010000000
VERSION     DAOMPD010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20730_length_2095_cov_2.511765"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLY93_13070"
     CDS             <1..766
                     /locus_tag="PLY93_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70456.1"
                     /translation="SVSYLCGLHIIQQYFNCLYVTALADAIMCTRFSVFLYILSFFIT
                     STACRNSVSNIDISHDIKYLAAFSSPSLVTPADENKFNQAFQSQYKTRVVLLRPITAE
                     ANLSLSSNKVSLFAFIKINAQREARIVAREIATALGGSLVQLYPIAESVNSQDNFSGN
                     TFYLSLEKRLSSFTRMNGALKQDAMLHEAESLSKDRNMLSYYAATILSNTPYHTLHIL
                     GYSGIAESQYLTLDGLFYRSIKKLDTADTVLLEKMR"
     gene            815..1204
                     /gene="gcvH"
                     /locus_tag="PLY93_13075"
     CDS             815..1204
                     /gene="gcvH"
                     /locus_tag="PLY93_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRP70457.1"
                     /translation="MAENPTELKYSKEHEWVKVDGTKARIGITHYAQDSLGDVVFIDF
                     PKEGATIAQNSVAGTIESVKAVSDIYAPLSGTITKINLGLNKAPAAVNQDPYGEGWLF
                     EIKDVNEAELASLLDAAAYEDFLKTLS"
     gene            complement(1244..1687)
                     /locus_tag="PLY93_13080"
     CDS             complement(1244..1687)
                     /locus_tag="PLY93_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70458.1"
                     /translation="MQRIKILFPLSVMALFALNCASGSNSKELLDEQGKVIGRYDRLN
                     DREAKASFDMNRNGVHEKVSNYKDAQLTSVEYYDDSNGTQTKTVGFKDGKPATVQVFD
                     KNGKAIRGDVLLDSQKGTTKEVTLPEKNKKVIFNGDGTVSISDVK"
     gene            complement(1724..>2095)
                     /locus_tag="PLY93_13085"
     CDS             complement(1724..>2095)
                     /locus_tag="PLY93_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70459.1"
                     /translation="RHVHYGVVALRDYFTKEISEARRRDREDWAYEIAIMLRNRFHFL
                     EIYEEHFGHAVSRNDWLKMLDASEVMGEFRKQLFTRMIPNLKEIGLLSERIRPKYAEL
                     GILKYETELSADKLTADDLTK"
BASE COUNT          563 a          494 c          426 g          612 t
ORIGIN      
        1 aagtgtcagt tatctctgtg ggttgcatat cattcaacag tattttaatt gcctgtacgt
       61 aacggcctta gcagatgcca tcatgtgtac gcggttctct gttttccttt atattctctc
      121 tttctttatt acaagcactg cttgtcgtaa ctcagtttca aatatcgaca tcagtcacga
      181 tattaaatac ttagcggcgt tttcgtcgcc ttctttagtt acccccgccg acgaaaataa
      241 atttaaccaa gcgtttcagt cgcaatataa aacccgtgta gtgctgctgc gccccattac
      301 cgctgaggca aatttatcct tatcctcgaa caaagtttct ctttttgctt tcattaaaat
      361 caatgcgcaa cgagaagcgc gtatcgtcgc gcgcgaaatc gccactgcac tgggcggttc
      421 gcttgtccag ctctacccca tcgccgaatc cgttaattca caagataatt tttcaggaaa
      481 cacgttttat ttgagccttg aaaaacgact ctcgtctttt acccgcatga atggcgcttt
      541 gaaacaagat gcaatgctcc atgaagcgga aagtctgtcg aaagatcgca atatgctttc
      601 atactacgcc gctacaatcc taagcaacac cccctaccac actttacaca tcttgggata
      661 ctctgggata gcggaatcgc agtatttgac attagacggt ctattttacc gttctattaa
      721 aaagctcgac accgccgata ccgtgctgct cgaaaaaatg agataatgaa ttcgtttatt
      781 ttctgaaaaa agtaatccat agagggagta aaaaatggcc gagaatccga ccgaattgaa
      841 gtatagtaaa gaacacgaat gggtaaaagt cgatggcaca aaagcccgca tcggtatcac
      901 acactacgcg caggactcgt taggcgatgt ggtttttatc gatttcccta aagaaggagc
      961 gaccatcgct caaaatagtg tcgctggcac gattgaatcg gtgaaagcag tgtcggacat
     1021 ttacgcacca ttgtcaggca ctatcacaaa aatcaatctg gggctaaaca aggcccccgc
     1081 agcagtgaac caagacccat acggtgaggg atggcttttt gaaatcaagg atgttaacga
     1141 agccgaactc gcttctcttt tagatgcggc ggcatacgaa gattttctga aaactctaag
     1201 ttgaacacta ccatcggctg ctttgcagcc gatggcggta ttattatttt acgtcggaaa
     1261 tggagaccgt tccgtcgccg ttaaaaataa ctttcttgtt tttctctggc aatgtgactt
     1321 cttttgtcgt gcctttttga ctgtccagaa ggacatcacc acggatagct ttaccgtttt
     1381 tatcaaacac ttgcactgtc gctggtttac cgtctttgaa accaacggtt ttagtttggg
     1441 taccgtttga gtcgtcgtag tattcaaccg atgtcaactg tgcatcttta taatttgaaa
     1501 ctttttcgtg aactccgtta cggttcatat cgaaactggc tttggcttct ctgtcgttta
     1561 acctatcgta acgaccaatg actttgcctt gttcatcaag aagttctttg ctattcgaac
     1621 cactcgcaca attcaatgca aataaggcca ttacgcttaa tggaaataaa atctttattc
     1681 tctgcatggt atagctcctc accgccgatt atatcgtcaa ttcttatttg gtcaaatcgt
     1741 cggctgttaa tttatcggca gaaagctcgg tttcgtattt taaaatacct aactcggcat
     1801 atttggggcg gatacgctca ctcagaagac cgatttcttt taagttgggt atcatgcgcg
     1861 taaagagttg cttacggaac tcccccatca cctctgaagc gtcgagcatc ttgagccagt
     1921 cgttacggct aaccgcatgc ccaaaatgtt cttcgtaaat ctccaaaaag tgaaagcgat
     1981 ttctaagcat gatagcaatc tcgtacgccc aatcttcgcg atcacggcgg cgtgcttctg
     2041 aaatttcttt agtgaagtaa tcacgcaagg caaccacgcc ataatggacg tggcg
//
LOCUS       DAOMPD010000479         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20789_length_2090_cov_2.374447, whole genome shotgun sequence.
ACCESSION   DAOMPD010000479 DAOMPD010000000
VERSION     DAOMPD010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20789_length_2090_cov_2.374447"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /gene="ccoN"
                     /locus_tag="PLY93_13090"
     CDS             complement(<1..1706)
                     /gene="ccoN"
                     /locus_tag="PLY93_13090"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRP70460.1"
                     /translation="MAVENGVLETFDYDNRIVRKFALATIVWGVIGMLVGVLIAILLY
                     APNYLVNHAVLSFGRIRPLHTNAVVFAFVGNAIFLGVYYSLQRLLKARMYSSILSNIH
                     FWGWQLIIVAAVITLPLGYTSSKEYAELEWPIDIAITVIWVVFGLNMLMTILNRREKH
                     LYVGIWFYIATFVTVAMLHIGNSLAVPVSFLKSYSVYAGVQDALVQWWYGHNAVAFFL
                     TTPFLGIMYYFVPKAANRPIYSYKLSIIHFWGLVFIYIWAGPHHLLYTSLPNWAQSLG
                     TVFSLMLLIPSWGGVLNGLLTLRGAWDRVRQDPILKFLIVGVTAYGMSVVEGPMMSLK
                     SVNALTHYTDYVVSHVHLGALAWNGGLTAAMLYYIIPRIYGRPLFSVRLANFHFWIAT
                     IGMIFYVVPLYVGGITQSLMWKQFTAQGTLQYANFLDTVTQIVPMYIMRSIGGSLYLI
                     GFLALAYNLFRTIRAGKFIPSETASAPPLKVAMAHVEKDTYKHRILERKPLVFTALTL
                     IAVMIGGIVEFVPVAMVRSNIPTISSVKPYTPLELEGRDIYIREGCSNCHSQMIRPFR
                     AET"
     gene            complement(1696..1911)
                     /gene="ccoS"
                     /locus_tag="PLY93_13095"
     CDS             complement(1696..1911)
                     /gene="ccoS"
                     /locus_tag="PLY93_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRP70461.1"
                     /translation="MEALYVLLIFSVLLGGGFLAAFFWAQKNGQFEDTQTPAMRILFD
                     EADAKRQREGQSHLLRKKQISGEYHGS"
BASE COUNT          614 a          520 c          505 g          451 t
ORIGIN      
        1 gtttccgcac gaaacgggcg tatcatctgc gagtggcagt tgctgcagcc ttcgcggata
       61 taaatatcgc ggccttcgag ttcaagcggt gtgtagggtt ttacactgga aattgtgggg
      121 atgttcgagc gcaccatcgc caccggtacg aattcaacga taccgcctat catcaccgca
      181 atgagagtaa gcgcggtaaa cactaagggt ttgcgctcta aaatgcggtg cttgtacgtg
      241 tctttttcaa cgtgcgccat cgccactttc aaaggtggag cactcgcggt ttcagaagga
      301 ataaacttgc ccgcacggat ggtgcggaaa aggttatacg ccaacgctaa gaaaccgatg
      361 agataaagcg aaccaccgat ggaacgcatg atatacatcg gtacaatttg agtcaccgtg
      421 tctaagaagt tggcatactg taatgtacct tgtgcggtga attgtttcca catgagcgac
      481 tgcgtaatac cgccgacata gagcggcacg acgtagaaga tcataccgat ggttgcaatc
      541 cagaaatgga agtttgccaa acgaacgcta aaaagtggac ggccataaat gcgcgggatg
      601 atataataga gcatcgcagc ggtgagacca ccgttccatg caagtgcacc caagtggacg
      661 tggctcacca catagtcggt gtagtgcgtg agtgcgttga cacttttaag cgacatcatc
      721 ggtccttcga cgaccgacat accgtatgcg gtaactccca caatgaggaa tttcaaaatc
      781 ggatcttggc gtaccctgtc ccatgcgccg cgcaatgtta aaagaccgtt caacacccca
      841 ccccacgaag ggataagtag catgagcgaa aaaacagtgc ctaaagactg tgcccagttg
      901 ggtagcgacg tataaagaag gtggtgtggc cctgcccaaa tataaataaa caccaagccc
      961 caaaagtgga taatcgaaag tttatacgag tagataggcc ggttcgccgc ttttggcacg
     1021 aaataataca tgatacccaa aaaaggcgtt gtcaagaaaa acgccaccgc gttatggccg
     1081 taccaccatt gcacgagagc gtcttgcacg ccagcgtaca ccgaataact tttgagaaac
     1141 gaaacaggta ccgcaagcga gttgccgata tgcagcatcg ccacggtcac gaatgttgcg
     1201 atataaaacc aaatacccac gtagaggtgt ttttcacggc ggttcaaaat cgtcatgagc
     1261 atattgagac caaacaccac ccaaataaca gtgatggcaa tatcaatcgg ccattcgagc
     1321 tctgcgtatt ctttgctcga ggtataaccc aaaggcagcg ttatcaccgc cgcaacgata
     1381 atgagctgcc aaccccaaaa gtggatgttc gagaggatgc tcgagtacat acgcgctttt
     1441 aagagacgct gtaacgagta gtacactcct aaaaaaatcg cattccccac gaacgcaaaa
     1501 acaaccgcat tggtgtgcaa aggccgaatg cgaccaaacg agagaaccgc gtggttcact
     1561 aaatagttgg gcgcataaag aaggatagcg atgagtactc ccaccaacat cccgatgaca
     1621 ccccagacaa tcgtcgccag agcgaacttg cgtacgatcc ggttgtcgta gtcgaaggtt
     1681 tctaaaaccc cgttttcaac tgccatgata ttctcctgaa atttgtttct ttcttaatag
     1741 atgactttgc ccttcgcgtt gccgctttgc atctgcctcg tcgaataaaa tacgcatcgc
     1801 tggcgtttgg gtatcctcga actggccatt tttttgcgcc caaaaaaaag cggctaaaaa
     1861 cccccccccc aaaaggacgc taaaaataag taatacatag agcgcttcca tattagaatt
     1921 tcccctgcgc cgttgtgtac gctttgaagc gcgacgatgc aaacgcaaac ccaatgacag
     1981 tgagcgaact gatgggcatg aggatagcgc aaaaaagcgg cgtcacttgt ccagtaagtg
     2041 cgacgctcac gccaaaaaca ttatatgcaa acgaaatgat gtacgcggtg
//
LOCUS       DAOMPD010000480         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_20958_length_2077_cov_2.095945, whole genome shotgun sequence.
ACCESSION   DAOMPD010000480 DAOMPD010000000
VERSION     DAOMPD010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20958_length_2077_cov_2.095945"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="PLY93_13100"
     CDS             <1..1353
                     /locus_tag="PLY93_13100"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRP70462.1"
                     /translation="DLKKIIERLTHAGTKKVRVAVADIDGVLRGKYLHIDKFTSALES
                     TFGFCNVVFGWDINDICYDNTKYTGWHSGYPDALVQVAPETERAVPWDNHVPFFLGEF
                     VNSDLSPLTICPRQTLKRVVEKARQMGFTAKNGMEFEWFNFRETPKNLEAKGYIHLEP
                     LTPGMFGYSLIRSGQEREYFNALMDELTEFGIPIEGLHTETGPGVYEAAICFSDALES
                     ADRATLFKTATKDIAARFGIMPSFMAKWHQTLPGCSGHMHQSLWNENGENIFYDAKDE
                     HHMSAAFKSYIAGQVKLLPEMLPFFAPTINSYKRLVDGYWAAVKPSWGIDNRTVSHRV
                     ILGSEKSTRVEVRISGSDVNPYLSLAASLAAGLWGIEQGLTLDKPPVRGNAFSESHAE
                     HLPRNLMDATRRLKESRTARIILGDELVDHFVATREWEWRQFQDAITDWETKRYFEIV
                     "
     gene            1420..>2077
                     /locus_tag="PLY93_13105"
     CDS             1420..>2077
                     /locus_tag="PLY93_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRP70463.1"
                     /translation="MAKDPKDLTPDNSTYAPWQREFFKNIDIFVKAGLKEDKAREMLI
                     KYLKLSSETPIPKVMETFKDETKLEEVGVFTERDDKLRDFMIDFLTPMLKKFHIEGEE
                     NLQDILPLLGKYPVTLVSNHLSHFDTAAMYGLLYNHSKEARRLADSMVFIAGRLVFLA
                     DFTRLGLYTIESLLVCSKRDMTDNPGMADMMTRINMRSFRQAADLQKQGKVICIFPEG
                     T"
BASE COUNT          534 a          506 c          510 g          527 t
ORIGIN      
        1 gatcttaaaa aaataatcga acggctaacg catgcaggga caaaaaaagt ccgcgttgcc
       61 gtggcggaca tcgacggtgt gctgcgcggt aaatatttac atatcgataa atttacctct
      121 gcactcgagt ctacatttgg attttgcaat gtagtctttg gttgggacat caacgatatt
      181 tgttatgaca atacgaaata caccggctgg cattcaggct atcccgatgc gctggtgcag
      241 gtggctcccg aaaccgaacg cgccgtgccg tgggataacc atgtgccgtt ttttttgggc
      301 gagttcgtga actccgattt atcgccctta accatttgtc ctcgacaaac cttaaaacgc
      361 gttgtagaga aagcgcgtca aatgggattc accgccaaaa atggcatgga atttgaatgg
      421 tttaattttc gtgaaacgcc taaaaattta gaagctaagg gatacatcca ccttgaaccg
      481 ttaactcccg gtatgttcgg ttactctctc atccgctcgg gacaagaacg cgaatacttc
      541 aacgcgctca tggacgagct cactgagttt ggcatcccca tcgaaggatt acacaccgaa
      601 accgggccgg gcgtttacga ggcggcgatt tgttttagtg acgctctcga aagcgccgac
      661 cgtgcgacgc ttttcaaaac tgcaaccaaa gacatcgctg cacgctttgg catcatgccg
      721 agctttatgg cgaagtggca ccaaacgcta cccggttgtt cgggacacat gcaccagtcg
      781 ctttggaacg agaacggcga aaatattttt tacgacgcta aagatgaaca tcacatgagc
      841 gctgcgttca agagctatat cgcggggcaa gtgaaacttt tacctgaaat gttacccttc
      901 tttgcgccga cgatcaactc gtacaagcgc ctcgtcgacg gctattgggc ggcggtgaaa
      961 ccgtcgtggg gtattgataa ccgcacggtg tcgcaccgcg tgatccttgg ttctgaaaaa
     1021 tcgacgcgcg tggaagtgcg tatttctggg tcggatgtca atccctactt gtcgcttgcc
     1081 gcctcgctcg cggcgggact gtggggtata gaacaaggct tgacgctcga caaaccaccg
     1141 gtgcggggaa acgctttttc agaaagtcac gccgaacatt taccgcgcaa cctcatggat
     1201 gcgacgcgac gcctcaaaga atcccgtacc gcgcgcatca ttttgggcga tgaactggtc
     1261 gatcattttg ttgccacacg cgaatgggag tggcgccagt ttcaagatgc tatcaccgat
     1321 tgggaaacca agcggtattt tgaaattgtg taatttgtcg gcggctcctg cggcacatta
     1381 ttcttgactt tgcgccgcgc tttttgtctt actcgcagca tggctaaaga tccaaaagac
     1441 ttaactcccg ataattcgac gtatgcaccg tggcagcgcg agttctttaa gaatattgat
     1501 atttttgtca aagcaggctt aaaagaagac aaagcgcgcg agatgcttat taaatatctc
     1561 aagctttctt cggaaacccc catccccaaa gtgatggaga ccttcaaaga cgagaccaaa
     1621 ctcgaagaag taggggtctt taccgagcgc gacgacaagt tgcgcgattt tatgatcgat
     1681 tttttgacgc caatgcttaa aaaattccat atagaaggcg aagaaaattt acaagatatc
     1741 ttacctcttt tgggaaaata tcccgtgacg ttggtttcaa accacttgag ccattttgac
     1801 accgcagcga tgtacggcct tttatacaac cacagtaaag aggcgcggcg tttggctgac
     1861 tcaatggttt ttatcgcagg ccgtctcgtt tttttggcgg actttactcg gttaggtctt
     1921 tacaccattg aaagtctgct cgtttgttct aagcgcgaca tgaccgacaa ccctggaatg
     1981 gcggatatga tgacccgcat taacatgcgc tcgttccgcc aagcagcgga tttacaaaaa
     2041 caaggcaaag ttatttgtat ttttcccgaa ggcaccc
//
LOCUS       DAOMPD010000481         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21013_length_2072_cov_2.862667, whole genome shotgun sequence.
ACCESSION   DAOMPD010000481 DAOMPD010000000
VERSION     DAOMPD010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21013_length_2072_cov_2.862667"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PLY93_13110"
     CDS             complement(<1..1151)
                     /locus_tag="PLY93_13110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRP70464.1"
                     /translation="MRSLLQRKMLLASVIFIAVLPILASASFITYRSFQQAKLDAVDR
                     AKTLLSHVTERQKQLIVQTLQSLRILTQLPDILAGKRECSLFLRKFIERNPYYVNAGL
                     VDAKGNIICSAQEPKHKINVSQREWFNMAMKTQVGQIGGLQFGLITGKPSIVAALPIV
                     EENLVTHVLYLSISVSWLEDVFAEYSLPEKSQITALDSHGLVLFQHPQKVENEPSLLG
                     KEFPSQKVWNHIKGSGENIVPARIYEDGGRYVYTFQRIEAEDRVAMVIALRFIESEIY
                     RQATRDGIALVLGLLLSLLSTLIIAYVAGNYLFLKPVAEEIDKLHDVAETDELTQVLN
                     RRGFVRLFKEKLEEEASNDYHALLLIDLDHFKNVNDTYGHSAGDAVLKET"
     gene            complement(1158..>2072)
                     /locus_tag="PLY93_13115"
     CDS             complement(1158..>2072)
                     /locus_tag="PLY93_13115"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRP70465.1"
                     /translation="LFLLVGEVLPKAIGRRKNLRLLEKSYPLIWVFYYLLLPMSYIFL
                     KISRFIGKVGVVRGEDARLEVFRFISEHTGESRLPLTESLAAYSSTMISEIMTPMNEI
                     SSLSVGATLGECAEIIEKSSYSRYPVYENIPAEIIGYLDLRDALSMPASARIRQIMKP
                     ALFYPYSMKLDALHAEMRRIAVPMVFVVSEYGIILGMVTEENLAEELVGDIFSHDQKL
                     ETEYLSKNEEGDYDIDCVMDIDDFAKVFSLTIIKENFETLGGYLLNNLMRVPVVNEEL
                     DLPPGKFKVLESTRSALRRVRFFPRRKR"
BASE COUNT          576 a          488 c          442 g          566 t
ORIGIN      
        1 gtttctttta agacggcgtc acccgcgctg tgcccgtagg tatcgtttac atttttgaaa
       61 tgatcgagat cgataaggag caatgcatgg tagtcgttac ttgcttcctc ttctaatttt
      121 tctttaaata gtcggacaaa accacggcgg tttaatacct gtgtcaactc gtcggtttcg
      181 gccacgtcgt ggagcttatc aatttcttcc gcgacaggct ttaagaaaag ataattaccc
      241 gcgacataag cgataatcaa tgtagataaa agtgaaagca aaagacccaa cacaagcgca
      301 atgccgtcgc gcgttgcttg tcgatagatt tcgctttcga taaatcgtaa tgcaataacc
      361 atcgcaacgc ggtcttccgc ttcaatccgc tggaatgtgt acacataccg ccccccatct
      421 tcataaatgc gggctgggac aatgttctcc ccagagcctt tgatgtgatt ccacactttt
      481 tggctgggga attccttacc caacaaagat ggttcgtttt caactttttg cggatgctgg
      541 aataatacca aaccatgcga atcgagcgcg gtgatttgcg atttttccgg aagcgaatac
      601 tcggcaaaaa catcctcaag ccaactaacg ctaatggaga gataaagcac gtgcgttact
      661 agattttctt cgacaatagg cagcgccgcc acaatgctgg gtttaccggt aataagccca
      721 aattgcaaac cgccgatttg ccccacttgc gttttcatcg ccatattaaa ccattcgcgt
      781 tggctcacgt ttattttatg tttcggttct tgagcgctgc agataatgtt cccttttgca
      841 tcgacgagtc ctgcattaac ataataggga tttcgttcga taaattttcg caagaatagc
      901 gaacattctc ttttgcctgc taaaatgtct ggcaactgag ttaatatacg caacgactgg
      961 agcgtctgca caatcagttg tttttgcctt tctgtgacat gcgataaaag ggtttttgct
     1021 ctatcgacag cgtctagttt tgcctgttga aaagagcggt aagtaatgaa cgaagcagag
     1081 gcgagtatcg gcaacacggc gataaaaatt accgaggcga gaagcatttt gcgttgaagt
     1141 aaactacgca ttttatttta tcgctttctg cggggaaaaa aacgaacccg ccgtaaagca
     1201 gaacgtgtcg attcaagaac cttaaatttg cccggcggca aatctaattc ttcattgacc
     1261 accggcactc gcatcaaatt attcaagaga tagcctccca atgtttcaaa attttcttta
     1321 atgattgtaa gggaaaatac tttggcaaaa tcgtcgatat ccatgacgca atcgatatca
     1381 taatcgcctt cttcgttttt ggagagatat tctgtttcca atttttgatc gtggctgaaa
     1441 atatcgccga cgagttcctc tgctaaattt tcttcggtga ccatccccaa aataatacca
     1501 tactcactta caacaaaaac catcggaacc gcaatccgtc gcatttccgc atgcagcgcg
     1561 tcgagtttca ttgagtatgg ataaaagaga gcgggtttca taatctgtcg aatgcgcgca
     1621 ctggcaggca tcgagagtgc atcgcgtaaa tccaggtagc cgataatctc agcggggatg
     1681 ttttcataaa cgggatagcg tgaataggag ctcttctcga tgatttcagc gcactcgccc
     1741 agcgttgcac ccactgaaag cgaagaaatt tcattcatgg gggtcatgat ttcgctgatc
     1801 attgtgctgg aatacgccgc caacgattcc gtcaacggta gacgactttc accggtgtgc
     1861 tccgagataa accgaaacac ttccaggcgc gcatcttcgc cgcgcacaac ccctacttta
     1921 ccgataaaac gcgaaatttt taaaaagata tagctcatcg gtaacaataa ataatagaag
     1981 acccaaatga ggggatagct tttttccaat aagcgcaaat tcttgcgtcg cccaatcgcc
     2041 ttgggtaaca cttcgcccac aagcaagaat aa
//
LOCUS       DAOMPD010000482         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21014_length_2072_cov_2.662866, whole genome shotgun sequence.
ACCESSION   DAOMPD010000482 DAOMPD010000000
VERSION     DAOMPD010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21014_length_2072_cov_2.662866"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLY93_13120"
     CDS             complement(<1..527)
                     /locus_tag="PLY93_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70466.1"
                     /translation="MLSGFERLSTQEDGERFSVCFDGALFPFYSRRSRPRDVALFKRA
                     IEETPPEMTPILVAPFIADVEEIFTLPRRFYFLAPLFFDTPQIDDARFIGDDAALHQF
                     LRGVESVDKFSIYLPPPWKEAAPYVETGVRQILAQAAARLKTIHHFARLWQTNLRVNA
                     RRVINYVDVRTLKESR"
     gene            complement(529..1029)
                     /locus_tag="PLY93_13125"
     CDS             complement(529..1029)
                     /locus_tag="PLY93_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70467.1"
                     /translation="MRWIYSVTVLSTLIFFPLCSEVNQFSFCYDFDRSGCIRPIAGDA
                     VFSTEKLQKKKTIADFANALYFRGDRLAFALNHARSKYNVTFECLRGEYTLPDTPGFK
                     EDIEYIELREKNVYGLVMAGSLVEKKYAAIKKETYRALLPFSVEYSIFCHKDLIAKHS
                     IRVVLK"
     gene            complement(1026..1499)
                     /locus_tag="PLY93_13130"
     CDS             complement(1026..1499)
                     /locus_tag="PLY93_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70468.1"
                     /translation="MTLASSITGALFIFQALHWFGVALYVGSLLFLLFIFQKVYQRYR
                     AYKYVDNFRAEVITLYWRYLHIAFLVIISSGTALALAKGKSVIQGAYGMVFSAKLALW
                     LVQIYFTQETLKPFVVDVESDEQPHTNTQPRVISPLFIFLLLMLISSAGFALKFL"
     gene            complement(1502..1855)
                     /locus_tag="PLY93_13135"
     CDS             complement(1502..1855)
                     /locus_tag="PLY93_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRP70469.1"
                     /translation="MIIIETLSHINMPAKELQKAIEFYTQFLDFEVVEENTNTAVVSF
                     DNLQVRLDSTLESYAKTPVLSFLLDVDDFTEALQEIEEENISIAKGPFEIDGGESVYV
                     ADPNGNLIELYYREE"
     gene            1978..2060
                     /locus_tag="PLY93_13140"
     tRNA            1978..2060
                     /locus_tag="PLY93_13140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2011..2013,aa:Leu,seq:caa)
BASE COUNT          602 a          466 c          504 g          500 t
ORIGIN      
        1 cggctctctt tgagcgtgcg tacatcgaca tagttaataa cacggcgtgc gttcacgcgc
       61 aggttcgttt gccacaggcg cgcaaagtgg tggatggttt tgaggcgcgc agccgcctgc
      121 gctaaaattt gccgcacccc cgtctcgacg tacggcgccg cctctttcca cggtggaggc
      181 aaataaatcg aaaatttatc cacgctttca acgccacgca aaaattgatg gagcgccgca
      241 tcgtcgccga tgaagcgtgc gtcgtcgatt tgcggcgtat caaaaaaaag gggggcgaga
      301 aaataaaacc ggcgtgggag agtaaaaatt tcttccacgt cagcgataaa cggcgcgaca
      361 aggatgggcg tcatttccgg tggagtttct tctatagccc ttttaaaaag cgcaacatcg
      421 cgcgggcgcg agcggcggct gtaaaaaggg aaaagtgcgc cgtcaaagca aaccgagaaa
      481 cgctcgccgt cttcttgtgt ggaaagccgt tcaaagccgg agagcatctt attttagcac
      541 aacgcggatg gagtgtttag cgattaaatc tttgtggcaa aaaatggagt actctacgct
      601 aaaaggaagt aatgcgcggt aagtttcttt ttttatcgca gcgtattttt tttcgacaag
      661 actgcccgcc atcacaagtc catagacatt tttttcgcgc agttcgatat actcgatgtc
      721 ttctttgaat cctggtgtat cgggaagcgt gtattcgcca cgcaagcact caaacgtcac
      781 gttgtacttc gagcgtgcgt ggttgagagc aaacgccaag cggtcgccac ggaaataaag
      841 cgcatttgca aagtcggcaa tcgttttttt cttttggagt ttttcggtgg aaaaaacagc
      901 atcccccgca atcggtctga tgcaaccact acgatcaaaa tcatagcaaa aggaaaattg
      961 gttcacctcg gagcacagag ggaaaaaaat gagcgtgctc aacaccgtta cagaataaat
     1021 ccacctcata agaactttaa ggcaaaacct gcgctggaaa tcaacattaa aagcaaaaag
     1081 ataaataagg gggatatcac ccgtggctgt gtattggtgt gtggttgttc gtcgctctct
     1141 acgtcgacga caaaaggttt gagcgtctct tgtgtgaaat aaatctgtac cagccaaagg
     1201 gccaactttg cggaaaaaac cataccgtaa gcgccttgaa tcaccgactt cccttttgcg
     1261 agggcaagcg ctgtaccaga ggaaatgatc accaagaaag cgatatgcaa atagcgccaa
     1321 tagagagtaa tcacttcggc gcgaaaatta tcgacgtact tgtatgcacg gtagcgttgg
     1381 tacacttttt gaaaaataaa aagtaaaaaa aggagcgaac ccacgtaaag ggcgacgcca
     1441 aaccaatgca atgcttgaaa gataaaaagc gcccctgtga tactggacgc taaagtcatg
     1501 gctattcttc gcggtagtaa agctcgatga ggttaccgtt ggggtcggcg acataaacgc
     1561 tttcgccccc gtcgatttca aagggacctt tggcgatgga gatgttttcc tcttcgattt
     1621 cttgtagcgc ttcggtaaaa tcgtctacgt cgagtagaaa agacaacaca ggggttttgg
     1681 catacgattc aagtgtacta tccaaccgca cttgtaaatt atcaaaactg acaacagcgg
     1741 tattggtgtt ttcttcaaca acctcaaaat ctaaaaattg ggtataaaat tcaatcgcct
     1801 tttgcaactc tttagcgggc atattgatgt gactaagggt ctcaatgata atcatgtaac
     1861 aggcaaaaaa cacgttaaaa acaggcgagc aagcactttt agtgaaatgg caaatcgata
     1921 taagcgttaa cttgctttgc gctatatctt aactttatag aatagcgctt acgtaccgcc
     1981 gaagtggtga aatggtagac acgccgcact caaaatgcgg tgggggcaac cccatctcgg
     2041 ttcaagtccg accctcggta cattgataaa tt
//
LOCUS       DAOMPD010000483         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21213_length_2056_cov_2.440280, whole genome shotgun sequence.
ACCESSION   DAOMPD010000483 DAOMPD010000000
VERSION     DAOMPD010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21213_length_2056_cov_2.440280"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PLY93_13145"
     CDS             <1..1556
                     /locus_tag="PLY93_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRP70470.1"
                     /translation="KAAPVFILMFATIVGFLYSRFLIGRRASRYLLWSAGFIFLFITL
                     LTLTAPDAAALTEKKVRFDFKTIFTNPLSELSHYFFKTAQPNRILENQSNNGINPALA
                     QSPLARYNTDSPTHARIFPRIAFQRSHKYNIVFYFLESTSYAYKNLKYNGMDIMPNLK
                     ALYEKGFATQKHYANFPLSINTFYTVLTAAYQYPDKNFIPCSRPQIPVPTIYETLKGA
                     GFQTAVFHGSSLDNFCRRDFLKNRHVDAQVGFGELDKMQYPPIDATWQADDRAMINPF
                     IEFMKQKPKKSKFAVVMPISPHHPYLVPSEQFNITKKELERNQGRKEQYWLQYINSLY
                     FSDFVLGEFIKAMEKNHLLKNTVLFIFGDHGEAFYQHAGNYLHSLFVYEENVRVPFVI
                     LNPVLFPQKIHYDGISRMIDIAPTALDIVGVKAPKHYQGLSLLSTHRQQYALLHTQWA
                     DDMMGIVDGNNKYIYRFADGFEELYNLSNDPTEKNNLATHERRLVDQYRSLVLNGRKA
                     SQEFYRALH"
BASE COUNT          595 a          438 c          414 g          609 t
ORIGIN      
        1 gtaaagcggc gcccgttttt attttaatgt tcgcgacgat tgtgggtttt ttgtattcac
       61 gatttctcat tgggcgtcgc gcgagtcgct atcttttgtg gagcgcaggc tttatctttc
      121 tttttatcac gttgttgacg ctgacagcgc ccgatgctgc ggcactcacc gaaaaaaaag
      181 tgcggtttga tttcaaaact atttttacaa atcctttaag cgaactgagt cattattttt
      241 tcaaaacggc acaacccaat cgcattttgg aaaatcaaag caacaatgga attaatcccg
      301 cattggcaca atcgccattg gcgcgctata acaccgattc acccacccat gcacgtattt
      361 ttcctcgaat tgcgtttcaa cgtagtcaca agtataacat tgttttttat ttcttggagt
      421 cgacatcgta cgcgtataaa aacctcaaat acaatggaat ggatatcatg ccgaacctca
      481 aggcgcttta tgagaaagga tttgcaaccc agaaacacta cgcaaatttt ccgttgtcga
      541 tcaatacttt ttacacggtg ctcacggcgg cgtaccaata ccctgataaa aattttattc
      601 cgtgttcgcg tccgcaaatc ccagtgccaa caatctatga aacgctcaag ggcgcgggat
      661 ttcaaacggc agtttttcac gggtcgagtc tggacaattt ttgccgccgc gatttcttga
      721 aaaacagaca cgttgatgca caagtggggt ttggcgaact tgataaaatg caatacccac
      781 ccatcgacgc cacttggcag gcagacgatc gagcgatgat taaccccttt atcgaattta
      841 tgaaacaaaa acccaaaaaa tccaaatttg ccgtggtaat gcccatctct ccacaccatc
      901 cgtatttagt accaagtgaa caatttaata ttacaaaaaa agaattggag agaaaccaag
      961 gacgcaaaga gcagtactgg ttgcaataca tcaactcact ttatttttca gattttgtat
     1021 tgggtgaatt tatcaaagcg atggagaaaa atcatttact caagaacaca gtgcttttta
     1081 ttttcggcga tcacggtgaa gcgttttatc aacacgcggg taactatctt cattcgcttt
     1141 ttgtctatga agaaaatgtg cgtgtgcctt tcgtgattct aaaccctgtt ctctttccac
     1201 aaaaaataca ttacgacggc atttcgcgca tgattgatat cgctcccact gcgctcgaca
     1261 ttgtgggcgt caaagccccc aaacactacc aagggcttag tcttttgtcg acgcaccggc
     1321 aacagtatgc gctattacac acgcaatggg cggatgatat gatggggatt gtcgacggta
     1381 acaacaagta catttatcgg tttgccgatg gttttgaaga actctacaat ttatccaacg
     1441 atccaaccga aaaaaataat cttgcaacgc acgaacgccg attggtcgac caataccgtt
     1501 cacttgtact caacgggcgt aaagcgtcac aggaatttta tcgtgccttg cattagagct
     1561 tcgagggttt ttcataatca aagccatcgc atttttgagt tttatctttc tttacctccg
     1621 gtagagcagt tttcagaaaa acaatcgact atgaaaaact ttcgagttta gcacagccga
     1681 tgaagtcgaa atctttgcaa ttggttcttg aatctgccaa aagagatatc aaaaagattt
     1741 gtcctgcccc attgttactg gattgtaaaa gatatctctg ctgactgggt tgaaagtgtt
     1801 gataaaacaa tttggatagc aagatcgtac cgttatatca cagtgtattg gtattccaaa
     1861 ccaagagcag ataaattcat caatgatgtg gtgtattagc agaattgttt caaaagctga
     1921 ctttatacca gatttttgtg ggagtaattc tgtcgatctt ttagattatg tcgcattagg
     1981 gggtaactat atcgattttt cagattatgt cgcattaggg ggtaactata ttgatttttc
     2041 agattatgac gcatta
//
LOCUS       DAOMPD010000484         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21320_length_2047_cov_2.602410, whole genome shotgun sequence.
ACCESSION   DAOMPD010000484 DAOMPD010000000
VERSION     DAOMPD010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21320_length_2047_cov_2.602410"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLY93_13150"
     CDS             <1..303
                     /locus_tag="PLY93_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRP70471.1"
                     /translation="AQPNDTMPAPRLGTGLKSPQGVHDLHGLIWEWVDDFNSSTVTGD
                     SRADTDTNSNMFCGATALTGSDLRNYTAYMRYGMRSSLKGNYTGLNLGFRCAKEIK"
     gene            300..887
                     /locus_tag="PLY93_13155"
     CDS             300..887
                     /locus_tag="PLY93_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRP70472.1"
                     /translation="MKKLQFVLAGLLAFGAMGACKEKDPHAHHKMESGNVSGASLYEF
                     HDMFTDQDGKKLMLHQMEGKYIVLSMFYATCTAICPRIASEMTRLEKALTNEQRAQTR
                     FVLISFDHERDDVKNLQKFKAKMKLDESFTLLTGSVDGVREVAAALGVNYKKLDDGEF
                     SHSSVFTLLSPKGEIILQHAGLSMNADTFREKIRP"
     gene            906..1304
                     /locus_tag="PLY93_13160"
     CDS             906..1304
                     /locus_tag="PLY93_13160"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HRP70473.1"
                     /translation="MTRLEWTIEYSVGDPKIDQQHAYLFELANRLFQHMGAPDAAQII
                     DDTVKELHRYVDTHFAYEEAILRKSKYEHYEEHKQLHELMRTRLDLLTKQLQQGVLNR
                     QDLVEFMENWLTEHIIMEDMRFISAVAKLS"
     gene            1397..>2047
                     /locus_tag="PLY93_13165"
     CDS             1397..>2047
                     /locus_tag="PLY93_13165"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRP70474.1"
                     /translation="MYNLPVFATAFRIFFLACALEAVAIISMWIATLHGVHVVETKIG
                     GVDWHSYEMVFGFTRAAIFGFVFTAGQHWSGKFLLGGKSALALFTLWFLGRFAFYLPE
                     PFAAVSYGFDIAAGAFAIYRLLPLLKQKNNRPPFFLIALMQMALVAAVVAHYVSAWQE
                     HFLHAVRVGLLTAVLLVAIIAGRILPFFAGVVMAGQKPKIRQGLEKWIWPATQLVVV"
BASE COUNT          509 a          451 c          500 g          587 t
ORIGIN      
        1 gcacaaccta acgacaccat gccggcgcct cgtttgggta cggggctaaa aagtccgcaa
       61 ggcgtgcacg atttacacgg gcttatttgg gagtgggtcg acgatttcaa ctcgtcaacg
      121 gtaaccggcg attcgcgcgc cgacaccgac accaactcga atatgttttg tggcgcaacc
      181 gctttaacgg ggagcgattt acgtaactac acagcgtaca tgcgctatgg tatgcgtagt
      241 tcgttgaaag gtaattatac aggccttaat ttaggctttc gttgtgcaaa ggagataaaa
      301 tgaaaaaatt acaattcgtg ttagcaggat tacttgcgtt cggcgcaatg ggcgcttgta
      361 aagaaaaaga tccccatgcg caccacaaaa tggagtcggg caacgtgtcg ggggcgtcgc
      421 tttatgagtt ccacgatatg ttcaccgatc aagacggtaa gaaattgatg ctacatcaaa
      481 tggaaggtaa atatatcgtg ctttcgatgt tctatgcaac gtgtacagcg atttgtcccc
      541 gcatcgcctc tgaaatgacg cgcttagaaa aggcgctcac aaatgaacag cgtgcacaaa
      601 cacgttttgt gttaatctca ttcgaccatg agcgcgacga tgtaaaaaat ttgcaaaaat
      661 tcaaagcaaa aatgaaactt gacgaatcgt tcactcttct tacagggagc gtagatggtg
      721 ttcgcgaggt ggcagccgca ttgggtgtaa actacaagaa gctcgacgac ggcgaatttt
      781 ctcactcgtc ggtctttacg ttactttcac cgaaaggtga aattatcttg cagcatgcgg
      841 ggttaagcat gaatgcagat acgtttcgtg aaaaaattcg cccttaactg tctcaaggag
      901 agtgcatgac ccgtttagag tggacgatcg aatatagcgt tggcgatccc aagatcgacc
      961 aacaacatgc ataccttttc gaactagcaa atcgtttgtt tcagcacatg ggggcgcccg
     1021 atgcggcgca gattatcgac gataccgtca aagaattgca tcggtatgtg gacacgcatt
     1081 ttgcatacga agaggctatt ctgcgtaagt cgaaatacga acattatgaa gagcacaagc
     1141 agctccatga gctcatgcgt acgcgtttgg acttgctcac gaaacaatta cagcaaggag
     1201 tgctcaatcg acaagatttg gttgaattta tggaaaattg gctcaccgag catattatta
     1261 tggaagacat gcgttttatt tcagcggttg cgaaactgtc gtaatatttt tgaatgatat
     1321 ttgcagaaat atactttttt gtcttttttt gatttacgtc aaggccgtag tttcataatt
     1381 tgtctttatt aacattatgt ataatttacc cgtttttgcg acagcgtttc gtatcttctt
     1441 cttagcgtgc gcgcttgaag ccgtggcgat catttcgatg tggattgcca ctttgcacgg
     1501 cgttcacgtt gtcgagacta aaataggtgg cgtcgattgg cactcgtacg agatggtctt
     1561 cggctttacc cgcgccgcta tttttggttt tgtttttacc gctggtcaac actggtctgg
     1621 taaattcctt ttgggcggca agtcggcgtt ggctcttttc actctctggt ttttaggtcg
     1681 gtttgctttt tatctacccg aaccctttgc cgccgtttcg tacggatttg atattgccgc
     1741 cggcgctttt gctatttacc gcttactccc cctcctgaaa caaaaaaata atcgtccccc
     1801 gttcttttta attgcactga tgcaaatggc gctcgttgcc gctgtcgtcg cacattatgt
     1861 aagcgcatgg caagagcatt ttttgcacgc agtgcgcgtg ggccttttga cggcggtgct
     1921 cttggtggca attatcgccg gtcgtatttt gccgttcttt gcgggtgttg tgatggctgg
     1981 tcaaaaacct aaaattagac aaggcctcga aaaatggatt tggcctgcaa cccaactggt
     2041 cgttgtc
//
LOCUS       DAOMPD010000485         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21370_length_2043_cov_2.392354, whole genome shotgun sequence.
ACCESSION   DAOMPD010000485 DAOMPD010000000
VERSION     DAOMPD010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21370_length_2043_cov_2.392354"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..812
                     /locus_tag="PLY93_13170"
     CDS             297..812
                     /locus_tag="PLY93_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HRP70475.1"
                     /translation="MKKIIALVMVGVWIATPAFAQSMRKHTDRKAKFSIDHPSNWQKT
                     LNKEGVNFTVKSKDNLANVQVMAAAVEADTSTVYFLSEVEKAASEQHINRIPEENRVV
                     ATDDLTAMNVDEGAIGAYILDYDGTKIKQLIMALRKNATMYTIIVTFAEVGEEQYKDI
                     AQRIGDSFKAL"
     gene            complement(861..1838)
                     /gene="nadA"
                     /locus_tag="PLY93_13175"
     CDS             complement(861..1838)
                     /gene="nadA"
                     /locus_tag="PLY93_13175"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRP70476.1"
                     /translation="MKSTEEKIVEWRKNAGKTYFKEELDAREPLVREILALKEQKNAI
                     ILGHNYMTPDVYYGISDFVGDSLALARKSLEVNADIVLFNGVHFMAETAKILNPKAKV
                     LIADLKAGCSLAESMTGADVRALRKKHPGVPIVSYINCTADVKTEVDIICTSANAVKI
                     VESFDSDTVIVAPDGYLANNIQKKTKKKIIPWAGKCMVHELYTEFDVDMARKSFPNAT
                     IIAHPECKDTVTAKVDFTGSTSQMGKFIRDSKAKEVMLMTECSMGDNLRADFPEVTFI
                     SSCQTCPHMKKITLEKVRDALKYEQFEITLSSETIERARISLDRMLTVA"
BASE COUNT          568 a          444 c          444 g          587 t
ORIGIN      
        1 tcgctggccg ctttgacgac gagaaagtaa aactcactgg gcgcaaggac actgttattt
       61 ttcacttgtt tctttatatc taccctggca ttattctttt ggttggcttt tttgtcgtgc
      121 gcgcgcgccg ccgtcgttta gatctaaaaa ccgatagata acgcttgctt ttttcagaat
      181 acgcttaact ttgccgaggg gatatcgcat tggctgccga tgtagtcgta tgctattgta
      241 tttttacgca ctacgtacat aaaaatacaa catcaaaaat tcctagagga gataaaatga
      301 aaaagataat agcacttgtg atggtgggtg tatggattgc aacccccgcc tttgcgcaga
      361 gtatgagaaa acacaccgat cggaaagcaa agtttagtat cgatcatccc agtaattggc
      421 aaaagacctt aaacaaagag ggagttaatt tcactgttaa atcaaaagat aatttagcga
      481 atgtacaggt catggcggcg gcggttgaag cggatacgtc gacagtctat tttttaagcg
      541 aagtggagaa agcggccagc gaacagcata taaaccgaat acctgaggaa aaccgcgtcg
      601 ttgcaaccga tgatttaaca gcaatgaacg tcgatgaagg agcaatcggt gcgtacatct
      661 tagattatga tggaacaaag attaaacaac tcattatggc gttacgtaaa aatgccacta
      721 tgtacacaat tatcgtgact tttgccgagg ttggcgaaga acaatacaaa gatattgcgc
      781 aaagaatcgg cgattctttc aaagctctct aacttatttt tcattctgcg cgcccatgcg
      841 cagaatggat ttacaaaaac ctaagcgaca gtgagcatgc ggtctaaaga aatgcgcgcc
      901 cgctcaatcg tttcactcga tagtgtaatt tcaaactgct cgtacttgag ggcatcgcgt
      961 actttttcga gagtaatttt tttcatgtgt gggcaagtct gacacgaaga aataaaagtt
     1021 acttcgggaa aatccgcgcg taagttatct cccatcgagc attcggtcat caacatcact
     1081 tccttggctt tggaatcacg gataaatttg cccatttgag aagttgaccc agtgaaatcc
     1141 acttttgccg tcacggtgtc cttgcattcg ggatgcgcaa tgatggttgc gttcggaaaa
     1201 ctcttgcgtg ccatatcgac atcgaactca gtgtaaagtt cgtgcaccat acatttcccc
     1261 gcccatggga tgattttctt ctttgttttc ttttggatgt tgttggcaag ataaccatcg
     1321 ggagcaacaa tcaccgtgtc agaatcgaaa ctctcgacga ttttaacggc gttggcgctg
     1381 gtacaaataa tatcgacttc tgttttgaca tcagcggtac agttgatata cgacacaatc
     1441 ggtacaccgg ggtgtttttt acgcagcgca cggacatccg ctcccgtcat gctttccgcg
     1501 agtgagcacc ccgccttgag atcggcgata agcacctttg ctttggggtt caggattttt
     1561 gccgtttccg ccatgaagtg cacaccgtta aagagaacga tgtccgcatt cacttcgagc
     1621 gacttgcgcg cgagcgccag agaatctccc acaaaatcag aaatgccgta atacacatcg
     1681 ggcgtcatat agttatgccc caaaataatc gcattctttt gttctttgag agcgagaatt
     1741 tcacgcacca gtggctcgcg cgcgtcgagt tcttctttga aataagtttt accggcattt
     1801 ttgcgccatt cgacaatttt ttcttcggtg gatttcatgc gttaaagaca aaatacgagt
     1861 atttgatagg attgtccact tttatagaac caggctctta tacttcaact gctgccgaat
     1921 tacaagtctc aagccgctat agcagcaccc tcaacctaaa aaaattattt tttttctcaa
     1981 ttttgcactt ttttttcaaa aagtgagaaa aattaagcgt tttctggtat ttataatata
     2041 tat
//
LOCUS       DAOMPD010000486         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21437_length_2038_cov_2.066062, whole genome shotgun sequence.
ACCESSION   DAOMPD010000486 DAOMPD010000000
VERSION     DAOMPD010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21437_length_2038_cov_2.066062"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..862)
                     /gene="panB"
                     /locus_tag="PLY93_13180"
     CDS             complement(71..862)
                     /gene="panB"
                     /locus_tag="PLY93_13180"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRP70477.1"
                     /translation="MNIQDFSKKKEAGEKITVVTCYDHAFARLIARTSIDAILVGDSL
                     GNVIQGNPNTLAVTLDEMVYHARMVKRGAPEVFVLVDLPFMAYQVSVEEALRSAGRIL
                     KETGADAVKLEGGKNVVPQVKAMVESGIPVVGHLGLTPQSVLSFGGYRVQGKTDEAAH
                     AMLEAAIALENAGAFALVLEMIPASLTTKISRALKIPTIGIGGGVESDGQVLVLNDLL
                     GLDPDFTPRFVRKYAHLSQNVLEALEHYASDVRTQQFPGEKETFS"
     gene            complement(938..1318)
                     /gene="folK"
                     /locus_tag="PLY93_13185"
                     /pseudo
     CDS             complement(938..1318)
                     /gene="folK"
                     /locus_tag="PLY93_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
     gene            1404..1841
                     /locus_tag="PLY93_13190"
     CDS             1404..1841
                     /locus_tag="PLY93_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70478.1"
                     /translation="MKKIYSTLLIIMSLVIFTHCEKHRHGDDHEHGHENSSGALRLDN
                     GKKWMADDITRQTYKGLNKDIRSAQLNDAKAIANFVAAQVKRVNALIQGCTMKGEAHN
                     QLHLLIEKTQKDLAAVKKTSPKNIEVVLHDLAKSTALFEKYFE"
BASE COUNT          533 a          536 c          439 g          530 t
ORIGIN      
        1 gccacagcaa accccagccc ttggcttgcc cccgtgatca aggcaaagcg cgcttcattc
       61 aagggaggtg tcatgaaaac gtttcttttt ccccggggaa ttgttgggtt cgcacgtcgc
      121 tcgcgtaatg ctcgagcgct tctaaaacat tttggcttaa atgcgcatac ttacgcacaa
      181 agcgtggggt aaaatcggga tctaagccca aaagatcgtt gagcaccaac acttgaccgt
      241 cgctttccac gccgccgccg atgccgatgg tggggatttt tagtgcacgc gaaattttgg
      301 tcgtaagcga cgcggggatc atttccaaca ccagcgcaaa cgcaccggcg ttttctaacg
      361 caatggcggc ttcaagcatg gcgtgcgccg cttcgtctgt tttgccctgc accctgtagc
      421 caccgaacga caacaccgat tgcggtgtga gtcccaaatg gcccaccacg gggatgccac
      481 tttcgaccat cgccttcacc tgcggcacta catttttgcc gccttcgagt tttaccgcgt
      541 cagcgccggt ttctttgagg atacgccctg cgctacgcag cgcctcttcg acgctcactt
      601 ggtatgccat gaacggtaaa tcgaccagca caaatacctc gggggctcct cgtttcacca
      661 tccgcgcgtg gtaaaccatt tcgtctaaag tcaccgcaag cgtgttgggg tttccttgga
      721 tgacgttccc caacgaatcg ccgaccaaaa tggcatcgat cgatgtgcgt gcgatgagac
      781 gtgcgaacgc gtggtcataa caagtcacca cggtgatttt ttcgcccgct tccttctttt
      841 ttgaaaaatc ttgtatattc ataaccgccc tactttgtat ttttttccac gctaatcccg
      901 tttcactctc gacgacatca ccgcataaat cgtgcatgat ggtgcgtatc catccgcgtg
      961 cacggttgaa gggatgaggt atcgtgagat ttttttcgtg aaaaccgccc cccgaccacc
     1021 aaataatatc aatatcgatt tcacgcggcc cattttttat ccgttttttt cgccccagtt
     1081 gtatttcaat tttttggatt tctgttaaaa gttctgtggg acttttattg gtggcaatca
     1141 aaagcccttg gttcaaaaac cacgcctgcg cccgaatgag gatgggcagt gtttctaaaa
     1201 ccggcgatat tttttttata acgcaaacat taccgatggc cttgagcgct tttcgtaaat
     1261 ttttttcacg gtcgccgaca ttcgtgccca gcgcgagtac atattcaaag gcggccataa
     1321 cattgttctt ggtaaaacaa aatctaaatg actatcagcg cgaaaagcgt ttacactcat
     1381 aaggcaaaac tcaaagtgca attatgaaga aaatatactc tactctcctt ataataatga
     1441 gtctcgttat tttcacccat tgtgaaaaac accgccacgg cgacgatcac gaacacggcc
     1501 atgaaaattc cagcggcgcg ttacgcctcg acaacggcaa aaaatggatg gccgatgaca
     1561 tcacccgtca aacctataaa ggtttaaata aagacatccg cagcgcacaa ctcaacgacg
     1621 ccaaagccat tgccaacttt gtggccgcgc aagtaaaacg agtgaacgca ttgattcaag
     1681 gttgcaccat gaaaggtgaa gcgcacaacc aactgcatct attgattgaa aaaacacaaa
     1741 aagatttggc ggcagtaaaa aaaacatccc ctaaaaatat tgaagttgtt ttacacgacc
     1801 tcgccaaaag caccgccctt ttcgagaagt attttgaata aagtcgcgtg ttttgcacgg
     1861 ggatttaaaa atctcacgct gactatacgt catgcgtaaa attatagcgg tacttctttt
     1921 tctctttgtg gttgcttcgc ttttagccct caaagaatat ctccttctta agaagattca
     1981 aacagcaatg gctgattcat cttcagaaga gagcaccctt cagacatccg tcacgaca
//
LOCUS       DAOMPD010000487         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21441_length_2037_cov_2.740666, whole genome shotgun sequence.
ACCESSION   DAOMPD010000487 DAOMPD010000000
VERSION     DAOMPD010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21441_length_2037_cov_2.740666"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2037)
                     /locus_tag="PLY93_13195"
     CDS             complement(<1..>2037)
                     /locus_tag="PLY93_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP70479.1"
                     /translation="ADALPDLGTTDMGNADALPDLGMDDAPADTAPAFDLPDTGGDFN
                     FDTPPAGGLDDASFNLDTPAETTPDLADFSSKPSAQQDDTYNFDSAGPSLDDLSPEVA
                     LAGDSGGLSSLSSDLSSLAAEDAATIDPATLRRVRETLRSYPTALRRRLSRALLDEGM
                     KPADSNELLQLLSEQASANEVGAWLDTKNVAEVAEEKYEEGPRIVMARPEYTEEGLAR
                     QERLIKLTRFGAIAAFVAFVLLGGAYFGLLKPYFYRQAVAKGRDIILQKGSAAIKEAE
                     QQFDKALTYYDNDTYAYLQYADAYRYKGMYTEAFAKLFADVKLLGASVKVGDKEMRSS
                     AELFGSLKRVPVVNYANGDKALNVGGAQLTLQKKGAYVVSHLDNHKDEAAVLLALGNF
                     HSNPSARFARTAYKNNYLGADYYRRVLMARPSTPAFKKEEMLDRAVMGIGDIFYHEKN
                     YDRALDYYKKIIDKEPDNVTAHAGVLKSLLKLFQLNNDPRMVIQHHTLIRGKKLENKL
                     PMYIKARLAAFYIDLPADNELRVKYNISPANMLTGQELKTRADELLTSIFNSSETDVF
                     GVKHEGSRFAEGYYQRARSYAKDKNQIRMALKQFEYAFHYDPRHFMALNDRAELLMSM
                     NDFSGAIDLLKRAKELSAPDRLAELGENEADETLSEAPVGIIPFNLGKAMYLDAL"
BASE COUNT          456 a          498 c          542 g          541 t
ORIGIN      
        1 cagtgcatcc aaatacatcg ccttacctaa attgaaaggg atgatgccca ccggcgcctc
       61 ggagagcgtt tcgtcggctt cattttcacc cagctcggct aaacggtcgg gtgcgctcaa
      121 ttcttttgca cgctttagta aatcgatggc tccagaaaaa tcgttcattg acattaaaag
      181 ttcggcacgg tcgttgagcg ccataaaatg gcgcgggtcg tagtggaacg catactcaaa
      241 ctgtttcaat gccatacgga tttgattttt atcttttgca tacgaccgcg cgcgttggta
      301 atatccctcg gcaaaccgtg aaccctcgtg ctttacgcca aagacatcgg tttcactcga
      361 attaaaaatg gacgttaaaa gttcgtctgc ccttgttttg agttcttggc cggtgagcat
      421 gtttgcaggt gagatgttat acttcacccg caactcgttg tcggcaggca ggtcgatata
      481 aaacgccgcc agccgcgctt taatatacat cggtaattta ttttccaatt ttttaccgcg
      541 gataagcgtg tggtgttgaa tcaccatacg gggatcgtta ttgagctgaa aaagtttcag
      601 taacgatttg agcacaccgg cgtgtgcagt cacattatcg ggttctttat cgataatttt
      661 tttatagtag tcgagagcgc ggtcataatt tttttcatgg tagaaaatat cgccaatgcc
      721 catgacggcg cggtcgagca tttcttcttt tttgaatgcc ggtgttgacg gacgcgccat
      781 gagcacccga cggtaataat cggcgcctaa ataattgttc ttgtatgcgg tgcgggcaaa
      841 acgcgcagat gggttcgagt gaaaatttcc caacgccaaa agcaccgccg cttcgtcttt
      901 gtggttgtcg aggtgagaga cgacgtacgc gccttttttt tgcaaagtaa gttgagcgcc
      961 acccacattc aacgctttgt ctccgtttgc atagttcacc acggggacgc gcttgagcga
     1021 gccgaaaagc tctgcgctcg aacgcatttc tttgtcgccg acttttacgc ttgcgccaag
     1081 caattttaca tcggcaaaaa gtttggcgaa cgcttcggtg tacatacctt tgtagcggta
     1141 tgcatcggcg tactgtagat aagcgtaggt gtcgttgtcg taatacgtga gcgctttatc
     1201 gaattgttgt tcggcttctt ttatcgctgc gctgcctttt tgtaaaatga tatcgcgtcc
     1261 ctttgcgacc gcttggcggt aaaaataagg ttttaataaa ccgaagtagg cgccgcctaa
     1321 aagcacaaac gcgacaaacg ctgcaatcgc gccgaagcgg gtgagtttaa tgaggcgttc
     1381 ttgccgcgcg agtccttcct ctgtgtattc gggacgcgcc ataacgatgc gcgggccttc
     1441 ttcatacttc tcttcggcca cctcggcgac attttttgta tcgagccatg cgcccacttc
     1501 gttcgccgat gcttgctctg agaggagttg caacaactcg ttcgagtcgg cgggcttcat
     1561 accttcgtcg aggagcgctc ggcttaaacg gcggcgtaat gcagtggggt atgagcgcaa
     1621 cgtttcacgc acgcggcgca gcgttgcagg gtcaattgtc gcggcgtctt cggcagcgag
     1681 tgaacttaaa tcgctggaaa gcgacgatag gccgccagaa tcgccggcta atgcaacctc
     1741 gggagataag tcgtccagag aaggtcctgc cgaatcaaaa ttatacgtat cgtcttgttg
     1801 tgcagacggc ttgcttgaga aatccgctaa gtctggggtt gtttccgcag gggtatctaa
     1861 attaaacgac gcgtcgtcga gcccccctgc gggtggagtg tcaaaattga aatcgccacc
     1921 agtgtcgggt aaatcgaacg caggagcggt gtcggcaggg gcatcgtcca tacccaagtc
     1981 ggggagtgca tcggcattcc ccatgtctgt tgtgcccaag tcggggagtg catcggc
//
LOCUS       DAOMPD010000488         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21462_length_2035_cov_3.321717, whole genome shotgun sequence.
ACCESSION   DAOMPD010000488 DAOMPD010000000
VERSION     DAOMPD010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21462_length_2035_cov_3.321717"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLY93_13200"
     CDS             <1..669
                     /locus_tag="PLY93_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP70480.1"
                     /translation="NPKGRLPVTFTALMNLKWDLRLTAVNQIEALGFKRSDVRHIVLT
                     HLDLDHAGGLPDFPKAKVHVYDDEYLAAMHPQSFNETQRYKSIQWAHNPDWQRYTLEG
                     ESWFGFPAVRALAGTGGDDILLIPVTGHTRGHVAVAVKGDKGWLLHCGDAYFHHGQMD
                     LAHEHCPIGLEIFQRLMAVDNDARVENLTRLRSLKQEQGHNVTLFSAHDKDEFERLAH
                     LKAV"
     gene            669..1487
                     /locus_tag="PLY93_13205"
     CDS             669..1487
                     /locus_tag="PLY93_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP70481.1"
                     /translation="MGSDTVQKSINHFFQKYLPDSGITRFFIGASNYYWFVRKVVSSA
                     LHLRGKHLDTFIRVVINQVRFTGLQALPMLSVIALSLGAVTIVEALVFLPKLGQNDFI
                     GNLLRLVIIREIGPLITAIIVLARSGSAITAELCTQKMHREIDSLEMMGINPYLMIVL
                     PRLFAGIISIMLLIIYFDVIAIMGGYFISLAVTSFPFSAFVDTLARSITMTDLVSTFI
                     KSVLMGIVIPLTCTYYGFKPESLFQIPIYVSKAVIYSLFTVFLLNAFISVLFYL"
BASE COUNT          484 a          432 c          470 g          649 t
ORIGIN      
        1 aatcccaaag ggcgcttgcc agttaccttt acggcactga tgaatctcaa gtgggattta
       61 cgcttaaccg ccgtcaatca aatcgaagcg ttgggtttta agcgtagcga tgtgcgacac
      121 attgttttaa cgcaccttga tttagaccat gccggaggtt tgcctgattt tcccaaagcg
      181 aaagtacatg tatacgacga tgaatatttg gcggcaatgc acccacagtc gtttaacgag
      241 acccaacgct ataaatcgat acagtgggcg cataaccccg attggcaacg ctacaccctt
      301 gagggagaat catggtttgg ttttcctgcg gtgcgtgcgc tcgcagggac agggggggac
      361 gatattttgt taatcccggt taccggccat acacgtggcc acgttgccgt tgctgtaaaa
      421 ggcgataaag gctggttgct ccattgtggc gatgcgtatt ttcatcatgg acagatggat
      481 ttagcgcacg aacattgccc cattggtctt gagatttttc aacggctgat ggcagtcgat
      541 aacgatgccc gtgtggaaaa tttaacacgc ctgcgcagtt tgaaacaaga acaggggcac
      601 aacgtgactc tctttagcgc acacgacaaa gatgagtttg aacgcttagc ccacttaaag
      661 gcagtatagt gggctcagac accgttcaaa aatcaatcaa ccatttcttc cagaagtatc
      721 tacctgactc tgggataact cgatttttca tcggtgcatc caactattac tggtttgttc
      781 gcaaagtggt cagctcggcg ttgcatttgc gcggcaagca cctcgacacg tttatacgcg
      841 ttgtcattaa ccaagtgcgt ttcacgggct tgcaagcgtt gccgatgctt tcggtgattg
      901 cgctttcgct tggtgcagtc actattgtcg aagcgttggt gtttttgccc aagctcggcc
      961 aaaatgactt tatcggcaat cttttgcgcc tcgtgattat tcgtgaaatt ggtccgctta
     1021 tcaccgcgat tatcgtattg gcgcgttcgg ggagcgccat cacggcagag ctatgcactc
     1081 aaaaaatgca ccgtgaaatc gattcactcg aaatgatggg gatcaatcct tacctcatga
     1141 ttgtattacc tcgtcttttt gcgggtatta tttcgattat gctgctcatc atttattttg
     1201 atgttattgc catcatgggt ggatatttta tttcacttgc ggttacatcg tttccgtttt
     1261 ctgctttcgt cgatacactc gctcgttcaa tcacgatgac cgatcttgta tcgacattta
     1321 ttaaatcggt tcttatgggt attgtcattc cactgacttg cacttattat ggctttaagc
     1381 ctgaatcact ttttcaaata cccatctatg tatcgaaggc ggtgatttat tctcttttca
     1441 ctgtttttct actcaacgca tttatatcgg tactctttta tttatgaagg cgtttcaagg
     1501 tagatttctt gcgttggttt tgtttgtttt ttgttcctgg ggaagtcctt gctatgcagt
     1561 tgtagcaacg ctccatacgg cgaatcccgt agaggaaaaa tctccttatg tagtggcgac
     1621 tattgatttc ttattgccag gttatggttt gtacacgcaa aatgagaaat ggttgggggg
     1681 gacgtacttt gtattgaatc ttgtttcgtt aactagccta tatttctcgt atcaacattg
     1741 gatttttagt aattctgcat acagtatcca tggagatgat agcagaattt atcgaggacg
     1801 tgcagaattt tactttgtag cgtctattct tgcaaatttt atgatccgtg ttataagttc
     1861 atggcatagt tactctctaa tgcgagagaa tgttgataag aaagggctac gatatgaatt
     1921 ctatccagat aacgcgaacg gtttttcagg acagattgga tattttttgc gattttaagt
     1981 ttttacgtag cgaccgccat cacgtcgagg atttgcatcg gagaaagccc tgctt
//
LOCUS       DAOMPD010000489         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21465_length_2035_cov_2.672222, whole genome shotgun sequence.
ACCESSION   DAOMPD010000489 DAOMPD010000000
VERSION     DAOMPD010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21465_length_2035_cov_2.672222"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PLY93_13210"
     CDS             <1..1483
                     /locus_tag="PLY93_13210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP70482.1"
                     /translation="DAKTASVPASSSQHPVALILAPTRELVVQIAEEAEFFADAVGLK
                     LATIFGGVDHDKQRRELARHPQIIVATPGRLLDFLRGGEINLKNVVRIVLDEADRMLD
                     MGFIDDVRKILSKCSSNESEERQFSLFSATINFSALYSVWEFMREPEEILINPELIDH
                     ASIEQEMLHLGQDEKLPYLIQFLEANKLEPVIIFTNSRQYVDVLVKNLNHHSIAAEGL
                     SSMVTQNRRLRVLDDFKESKFRVLVATDVASRGLHIEDVQLVVNYDIPMDSETYVHRI
                     GRTARAGKTGKALSFSSEFDYDSLAKLERYLKYKIPVAQPEQRFIDNLSFVRIVQVAR
                     QDGVDHTRSRSGRRTDSKRREQKRGQQKTGARTQTTPQREKTGQKQRHGARQEQKRDG
                     GAYYDSRRRNERPVQVQQKQQENFAIVSRDTKKAGVFSRMLTSLKSLFVKKKKPQLSE
                     RTKQILAQENHGNRTQTSGTRRHNNKRRNNAHNRGKKNNNRRR"
     gene            1483..>2035
                     /locus_tag="PLY93_13215"
     CDS             1483..>2035
                     /locus_tag="PLY93_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70483.1"
                     /translation="MMMLLSERTLTGKLIFLTLALFSFFLPLHFFTSTVLLAAASLFF
                     LISLCFREARLALHFAAAPRLRNLFFVLFLFALWRLFYGARTDGQSDVFASVRGVWFL
                     LIPALIYFYASSRSRLYTLVTFFLAASAFAVLGNVWSTRAIFLEHPLQSFTATPATLY
                     ATAFVLLLGVVEASFFASHKVRGS"
BASE COUNT          513 a          527 c          490 g          505 t
ORIGIN      
        1 agacgcgaag acggcatccg tgccggcgtc ttcttcgcaa catcctgttg ctctaatcct
       61 tgcgcccact cgggaattgg tggtgcaaat tgcagaggaa gcggagtttt ttgcggacgc
      121 agtgggatta aaactggcca cgatttttgg cggtgtcgac cacgataaac agcgccgtga
      181 actggcgcgg catccacaga ttattgtagc aacccccggg cgacttctcg actttctccg
      241 tggcggtgaa attaatttga aaaacgtggt gcgcattgtc ctcgatgaag ccgaccgcat
      301 gctcgacatg ggcttcatcg acgacgtacg caaaatcctg tcgaagtgtt cctccaacga
      361 aagcgaagaa cgacagtttt ctcttttctc cgccaccatc aatttcagcg ctctctattc
      421 ggtgtgggag ttcatgcgtg agcctgaaga aattctcatt aaccccgagc tcatcgatca
      481 cgcgtcgata gaacaagaaa tgttgcattt aggacaagat gaaaaattac cgtacttaat
      541 ccaattttta gaagccaata aactcgaacc agtgattatt tttaccaaca gccggcagta
      601 cgtggatgtt cttgtgaaaa acttgaatca ccacagtata gccgccgagg gtttgtcgag
      661 tatggtgaca caaaatcgtc gtttgcgtgt actcgacgat ttcaaagaaa gtaaattccg
      721 cgtcttggtt gctaccgacg tggcgagtcg aggcttgcac atcgaagacg tacagcttgt
      781 cgtgaattac gacatcccca tggattctga gacgtacgtg caccgcattg gacgcacggc
      841 gcgcgcaggc aaaaccggca aggcgttgag ttttagctcg gagttcgatt acgattcgct
      901 cgcaaagctt gaacgttact tgaaatacaa aattccggtt gcgcaacccg aacaacgctt
      961 tatcgataat ttaagttttg tgcgcatcgt gcaagtggcg cgccaagacg gtgtagacca
     1021 tacccgttca cgcagtgggc gccgcaccga ctctaaacga cgcgagcaaa aaagaggtca
     1081 gcaaaaaacc ggcgcaagaa cacaaaccac cccgcagcgg gaaaaaaccg gacagaagca
     1141 acgccatggc gcacgacaag agcaaaagcg cgacggtggt gcatactatg attcacgccg
     1201 ccgcaacgaa cgaccggtgc aggtacagca aaagcaacaa gagaattttg ctattgtctc
     1261 gcgcgacacg aaaaaagcgg gtgttttttc acggatgttg acttcgttga agtccctgtt
     1321 tgttaagaag aaaaagccac aactttccga gcgcacaaaa caaattcttg cccaagaaaa
     1381 ccacggcaac cgcacacaaa caagcggtac acgccgtcac aacaataaac ggcgtaacaa
     1441 cgcacacaac aggggcaaga aaaacaacaa ccgacggcga taatgatgat gctgctttct
     1501 gaaagaacgc tcacaggaaa gttaattttt ctcacacttg cgctattttc gttcttttta
     1561 ccgttgcatt tttttaccag cactgttcta ctcgcggccg cctcgttatt ttttttaatc
     1621 tctctctgtt ttcgtgaagc gcggctcgcg ttacacttcg ccgccgcccc ccggttgcgg
     1681 aatctttttt tcgttctttt tctttttgca ttgtggcgcc ttttttacgg tgcgcgcacg
     1741 gacggccaaa gcgacgtatt tgcaagcgtg cggggagtat ggtttttact cataccagcg
     1801 ctgatttatt tttatgcttc atcgcgctcg cgtctttaca ccctcgttac atttttttta
     1861 gccgcgtccg cctttgccgt gctgggcaat gtttggtcaa cgcgcgcgat atttcttgaa
     1921 caccccttgc agagttttac cgcaaccccc gcaacactct acgcgacggc gtttgtcttg
     1981 ctcttaggcg ttgtggaagc gtcgtttttt gcgagtcaca aagtgcgtgg ctccg
//
LOCUS       DAOMPD010000490         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21559_length_2028_cov_2.501267, whole genome shotgun sequence.
ACCESSION   DAOMPD010000490 DAOMPD010000000
VERSION     DAOMPD010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21559_length_2028_cov_2.501267"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..633)
                     /locus_tag="PLY93_13220"
     CDS             complement(64..633)
                     /locus_tag="PLY93_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70484.1"
                     /translation="MTWFHLWRIRRKINKAIKKLLHNLHHDSKSYLEYGNLRRGKPWG
                     LREGREIVWIFLVDLLAERPLIYKITYHIDEGRYHCYSRSGGRNEMTNVLQFSSDSLD
                     ELIERLNMLASEVPAIRERMLRQYIYSMKNQKTSKRWMDFFKKKDNQLNADTLLHLKE
                     AVHAMEGQHKISKGEAMKLLGYVEMLFDE"
     gene            complement(723..1208)
                     /locus_tag="PLY93_13225"
     CDS             complement(723..1208)
                     /locus_tag="PLY93_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRP70485.1"
                     /translation="MKIEENKQGLFIMSSGAAAQPFQYAVGLYGSKFLQELRDHHRIL
                     GICCPGCKKVYVPPRQVCGPCYKEMRELVEVATEGKIVSYTILRFQFLDPETGEKKPV
                     PYGYGFFKLDGADNYFQHFMEVPEDESRLYIGARVKAIFSNEKHNSLKAIKHFALIEA
                     K"
     gene            complement(1256..>2028)
                     /locus_tag="PLY93_13230"
     CDS             complement(1256..>2028)
                     /locus_tag="PLY93_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRP70486.1"
                     /translation="IWDRLVFAGAIGGLALEAQAYLARYNHKKEDAARVVVRDRKNGA
                     NNPYAHVQKETTIKQVLGSPLIADPLHWLDMCPRTDGAAAVIFAADTIAEKICDKPAW
                     VLGTAVRHNWAMTADVPLHGLQSLTSASKELFTKTGITDPLKQLDVIEMYLPYSYAGL
                     SWIESLGLCKPGDGPKLVWDGVTDMEGELPINPSGGPICGNPIGATALIRVAEATLQV
                     QGRAETRQVKGGKVEIALATGFGGCFWSDMLLFGSKKP"
BASE COUNT          494 a          504 c          474 g          556 t
ORIGIN      
        1 aagcagggct ttctccgatg caaatcctcg acgtgatggc ggtcgctacg taaaatacaa
       61 ggtttactca tcaaacaaca tctctacgta accgagcaac ttcatagctt cacctttaga
      121 aattttatgt tgtccttcca ttgcatgcac tgcttctttg aggtgtaaca gtgtgtcagc
      181 attgagttga ttgtcttttt tcttaaagaa atccatccat cttttggatg ttttctggtt
      241 cttcatcgag tagatgtatt ggcgtagcat ccgttcgcgg atggcgggaa cctcagaggc
      301 gagcatattc agtcgttcga tgagttcatc taaactatcg gaagaaaact gcagtacatt
      361 cgtcatttca ttgcgtcccc ctgagcggga ataacaatgg tatcgccctt cgtcgatatg
      421 gtacgtaatt ttatagatga gagggcgctc tgccaagaga tcgactaaga aaatccacac
      481 gatttcacgg ccttcgcgca atccccaggg cttccctctg cgtagattgc catattctaa
      541 gtaggatttt gagtcatggt gcaggttatg cagtaacttt ttgatggctt tattgatttt
      601 acgccggata cgccaaaggt ggaaccacgt caaacgacga taagcatatt ttatttcgcg
      661 tagccccccc acttttccct aaaaaacttc aaattttcat taactaacca gcagacatgg
      721 ggctatttag cttcgatgag cgcaaaatgc ttaattgcct tcagcgaatt atgtttttcg
      781 ttgctaaaaa tagccttcac ccgggcacca atataaagcc gcgattcatc ctcgggcact
      841 tccataaaat gctgaaagta gttatccgca ccatcgagtt taaagaaacc atagccatac
      901 ggcaccggct ttttctcacc agtctcaggg tctaaaaact ggaaacgcaa gatagtgtag
      961 ctgacaatct tcccttcagt ggcaacttcg acaagctcgc gcatttcttt atagcaaggc
     1021 ccacatactt gccgtggtgg cacatacact ttcttgcaac cgggacagca gatccccaga
     1081 atacggtggt ggtcgcgcaa ttcttgcaaa aatttgctac cataaagacc cactgcgtat
     1141 tgaaacggtt gcgccgccgc gcccgacgac ataatgaata gtccttgttt attttcttca
     1201 attttcatgg gtgtctcctt gtgtagttat tcataaaaaa gtgggatgca ctctgctatg
     1261 gctttttgct cccgaaaagc agcatgtccg accaaaaaca gccaccaaaa ccagtagcaa
     1321 gtgctatttc tactttaccc cccttgactt gccgggtctc ggcgcggcct tgcacttgca
     1381 gcgtggcttc ggcgacgcgg atgagtgctg tggctccaat gggattgccg caaatcggac
     1441 cgcccgaggg gttaatcggg agctcacctt ccatgtctgt gacaccgtcc cataccagtt
     1501 tgggaccatc gccaggttta caaagcccta aggactcaat ccagcttagt cctgcatacg
     1561 aataagggag gtacatttca atcacatcga gttgttttaa gggatcggta atccctgttt
     1621 tagtgaagag ctcctttgat gcagaggtga gcgattgtag gccatgtagt ggcacgtcag
     1681 cagtcatcgc ccagttatgc cgcaccgcag tgcctaaaac ccacgcgggt ttgtcgcaaa
     1741 ttttttcggc gatggtgtcg gcggcaaaaa tgaccgccgc tgcaccatcg gtgcgcgggc
     1801 acatatcgag ccaatgtaat ggatcggcga tgagaggcga gcccaatact tgtttaatgg
     1861 tggtttcttt ttgtacgtgc gcgtaggggt tgtttgctcc atttttccgg tcacgcacca
     1921 cgacgcgtgc ggcgtcttct tttttgtggt tatagcgtgc aaggtacgct tgtgcttcga
     1981 gcgcaaggcc accgatggcg ccagcgaata caagacgatc ccatatcg
//
LOCUS       DAOMPD010000491         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21609_length_2023_cov_2.806911, whole genome shotgun sequence.
ACCESSION   DAOMPD010000491 DAOMPD010000000
VERSION     DAOMPD010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21609_length_2023_cov_2.806911"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLY93_13235"
     CDS             <1..340
                     /locus_tag="PLY93_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRP70487.1"
                     /translation="EEASKSEKLLLDCGGGIVEEADGLRSERKIKLLKENFFCIYISL
                     SEEKLLHRLENLAHNASRPRFSNTDSPQSLLAVFKRREPLYLETAHAVVDISDTNIQE
                     SALRITQLFK"
     gene            350..1786
                     /locus_tag="PLY93_13240"
     CDS             350..1786
                     /locus_tag="PLY93_13240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRP70488.1"
                     /translation="MFRVSLITRNRTLVDFCKNNLPHGFTLKNSTRFSWGRDNLQGII
                     LFDSDFLGEDQAAILPALAEHFNGKESAVSLCLMMNQETKARTLSLFPEKLEKIQGIL
                     SYRIENGKVAQLTSTEWQWFLETQQKILKQVSERILAQTENEILAARLSQISSQRNVE
                     IRFPAFMHGKSLSIARFREKLVAVSTAEAYLFINSKGDIPIDDFLEYYASLIRPETPP
                     RWRHIDFAKTPPHLHAQQLWPTKKSIKTAFSILCLQNLHLLEWQNQAVLSNQIRNLND
                     SSLRLIVVGNTELPQLVRRDLFRQDLYSLLRKTMIEIPPLYERIEDISHIAAEYIQRK
                     NFTHLSEDKVAVAKKILNRFDVSTGYKGLFMTLDLMNELKKSKGMPVFELMQETSVNE
                     EFKAAQAFLREEVEADPASLFDNLASGEKNSMSLENVEKHYITAVCQRYGWQVTEAAR
                     HLQISRKTLYDKMRRYKISRPDKKKRAS"
     gene            1783..>2023
                     /locus_tag="PLY93_13245"
     CDS             1783..>2023
                     /locus_tag="PLY93_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRP70489.1"
                     /translation="MKRSAFHIASGKFKRIAFNPPKAHGHHQNTTTARVKEAAFQIVR
                     NNIDLEGGWLFYDLFAGSGQMGLEALSLGATHATFV"
BASE COUNT          589 a          478 c          440 g          516 t
ORIGIN      
        1 cgaagaagct tcaaagagcg aaaaattgct tctcgattgc ggcggtggca tcgtcgaaga
       61 ggcggatgga cttcgtagcg aacgaaagat taaactactt aaagaaaact ttttttgtat
      121 ctatatttcg ctcagcgaag aaaaattact ccatcggctg gaaaatttag cgcataacgc
      181 gtcgcgtccg cggttttcaa ataccgattc accgcaaagc ctgctcgccg tctttaaaag
      241 gcgcgaaccg ctataccttg aaacggcgca cgccgtcgtc gatatttcag acaccaatat
      301 tcaagagagc gctttgcgca ttacacagct cttcaagtaa ggtcattgaa tgtttcgtgt
      361 ttcgctaatc acccgcaacc gaacccttgt tgatttttgt aaaaataatc tgccacacgg
      421 tttcactctc aaaaactcta cacgattcag ctgggggcgc gacaatttac aaggtattat
      481 tcttttcgat tccgattttt taggtgaaga ccaagctgcc attttacctg cactcgccga
      541 acactttaac ggcaaagaaa gcgccgtgtc gctttgtctt atgatgaatc aagaaacaaa
      601 agcacgcacg ctttctcttt tccctgaaaa acttgaaaag attcaaggca tactatcgta
      661 tcgcattgag aacggcaaag tcgcgcagct cacctccacc gaatggcaat ggtttcttga
      721 aacgcaacaa aaaatcttaa aacaagttag tgaacgtatt ttggcacaaa cagaaaatga
      781 aattttagcg gcgcgcttat cgcaaattag ttcacaacgg aatgtggaaa ttcgctttcc
      841 cgcgtttatg cacgggaagt cgctaagcat cgctcgcttt cgtgaaaaat tagtcgcggt
      901 gagtactgct gaagcgtatt tatttatcaa tagtaaaggc gatatcccca tcgacgactt
      961 tttagaatac tatgcctcgc tcatccgacc cgaaacgccg ccgcgttggc ggcatatcga
     1021 tttcgctaag acaccgcccc atctacatgc gcaacaacta tggccgacga agaaatccat
     1081 aaaaacagct ttttctattt tgtgtttgca aaacttacat ttattggaat ggcaaaatca
     1141 agcggtattg agtaaccaaa tccgcaactt gaacgacagt agtttaagac tcatcgttgt
     1201 gggtaataca gaactcccac agcttgtgcg gcgcgatctt tttagacaag acctctactc
     1261 attgctgcgc aaaaccatga ttgaaattcc accgctttat gagcgcattg aagatattag
     1321 ccacatcgcc gctgaatata ttcagcgcaa aaatttcact cacctttccg aagataaagt
     1381 ggcggttgcg aaaaaaatac taaaccgctt cgatgtttca acaggctaca aggggctttt
     1441 tatgactctt gatctaatga acgaacttaa aaaaagtaaa gggatgcctg tttttgaact
     1501 gatgcaggaa acttccgtca acgaagaatt taaggctgcg caggcgtttt tgcgcgaaga
     1561 ggtcgaggcc gatcccgcat cgcttttcga caatttagcc tcgggtgaaa aaaattcgat
     1621 gagtcttgaa aacgtggaga agcactacat taccgccgtt tgccaacgat atggttggca
     1681 agtgaccgaa gccgcgcgcc atttacaaat ttcacgcaag acgctctacg ataaaatgcg
     1741 ccgctataaa atctcgcgcc ctgacaagaa aaagcgcgcg tcatgaaacg cagcgcgttt
     1801 catatcgcat cgggaaaatt taagcggata gcgttcaatc ctcctaaagc gcacgggcac
     1861 caccaaaata ccaccactgc gcgcgtcaag gaagcggcgt ttcaaattgt gcgtaacaat
     1921 atcgatttag agggcggttg gcttttttac gatctttttg cgggtagcgg tcaaatgggg
     1981 cttgaagcgt tgagcttagg cgccacgcat gccacctttg tcg
//
LOCUS       DAOMPD010000492         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21723_length_2015_cov_2.735204, whole genome shotgun sequence.
ACCESSION   DAOMPD010000492 DAOMPD010000000
VERSION     DAOMPD010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21723_length_2015_cov_2.735204"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLY93_13250"
     CDS             complement(<1..349)
                     /locus_tag="PLY93_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP70490.1"
                     /translation="MPTIKSTIRMPQKIKSIFPLAERIGAGVLDLLESLGAFVIFAGQ
                     TFRYIFARPFDFRNLLYQMGEIGVKSIPVGVVSSFFVGMVMALQLGGPMEQQIQGIST
                     FMGGGISLAMVREL"
     gene            442..654
                     /locus_tag="PLY93_13255"
     CDS             442..654
                     /locus_tag="PLY93_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70491.1"
                     /translation="MANEPKKPLIDKLLIIGALVTFITVTILFSPMLLMATVLAGLPM
                     VAMVILITPTLHLNILERQTESNREK"
     gene            719..1819
                     /gene="ribB"
                     /locus_tag="PLY93_13260"
     CDS             719..1819
                     /gene="ribB"
                     /locus_tag="PLY93_13260"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRP70492.1"
                     /translation="MLKKLSQIPLLRKSVSGIVDVPTALAAFKAGKMLIVTDSEDREN
                     EGDLVISADDSTPETVNFMAKFGRGLICVPVTRNRAEKLNFQPMVEHNEDARRTAFTV
                     SVDAKEKTSTGISAHDRYYTIKKIADGASRANDFMRPGHIFPLVAVEGGVLVRAGHTE
                     ASVDLCRLTGKSPVGVICEIMKDDGTMARMADLKKFSKDHHLPILTIEALIEYRRKSE
                     QTVAFSHKDKLKTKWGDFTAHYFNSAIDANVHVALSRGKIDGKEPVLVRVHREDVLAD
                     VFQITPRKNYMAVDEAMAALAKAKRGVFLFLGQKSSQLIPESDRTLREYGIGAQILTH
                     LGVKKIKLLTRNPRRVVGLDAFGLEIVETVTE"
     gene            1816..>2015
                     /gene="ubiA"
                     /locus_tag="PLY93_13265"
     CDS             1816..>2015
                     /gene="ubiA"
                     /locus_tag="PLY93_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UbiA prenyltransferase family catalyzes the
                     transfer of a prenyl group to various acceptors with
                     hydrophobic ring structures in the biosynthesis of
                     respiratory quinones, hemes, chlorophylls, vitamin E, and
                     shikonin; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRP70493.1"
                     /translation="MKQAAAYLRMIKFSHTLFALPFALAALVIIYARYKSQVHITPLK
                     VLLLILAFTGMRSFAMAINRIL"
BASE COUNT          541 a          483 c          483 g          508 t
ORIGIN      
        1 caagttcacg caccatcgcg agcgaaattc cgcctcccat gaacgtagat atcccttgga
       61 tttgttgctc catcgggccg cctaactgga gcgccatcac catacctaca aagaacgaac
      121 tcaccacacc gacggggatc gatttcactc cgatttcccc catctggtag aggagattgc
      181 ggaaatcaaa aggtcgagcg aatatgtacc gaaaagtttg ccccgcgaaa ataacgaatg
      241 cgcccaaact ttcgaggagg tctagcaccc ctgcgccaat tctttcggcg agagggaaaa
      301 tacttttgat tttttgtggc atacgaatcg tcgactttat ggtcggcata aagagtctgt
      361 gcgtcaatcg gaaagttgac gcacaggctc acaaacctcg aaaggcattg acgggtcatt
      421 gacaggacaa taaacgacgg catggctaac gaaccgaaga aacctctcat tgataaatta
      481 ctcattattg gcgcattggt gacatttatt actgtcacaa ttctgttctc gccaatgctt
      541 cttatggcaa ctgttttggc gggactaccg atggtggcaa tggtgatttt gattacccca
      601 acgctgcatc tcaatatcct cgaaagacaa acagaatcca accgcgaaaa ataattttca
      661 tctattaagt ttctgatatt ttttgtgcgc taagcgcaca aaaaattatt tcactgttat
      721 gttgaaaaaa ctttcccaaa ttccattatt gagaaaatca gtttcgggta ttgtcgacgt
      781 gcccaccgcg cttgctgctt tcaaagcagg caagatgctc attgtcactg attcggaaga
      841 ccgtgaaaac gagggcgatc tcgtcatttc cgccgacgac tccacgcccg aaaccgtcaa
      901 ttttatggcg aaatttggca ggggactcat ttgcgtgccg gtgacgcgta accgcgctga
      961 aaaacttaat tttcaaccga tggttgagca caacgaagat gcccgccgca ctgcatttac
     1021 cgtttcagtc gatgcaaagg aaaaaacatc cacgggtatt tcagcgcacg atcgttacta
     1081 tactataaaa aagattgccg acggcgcatc gcgcgcgaac gactttatgc gtcccgggca
     1141 tatttttccg ttggtggcgg tggaaggcgg agtgttggtg cgggctggtc acaccgaagc
     1201 gtcggtcgat ttgtgtcgct taacgggtaa atcccccgtg ggcgtgattt gtgaaatcat
     1261 gaaagacgac ggcacgatgg cgcgcatggc cgatttgaaa aaattttcca aagatcacca
     1321 tctgccaatt ttaacgattg aagcgctcat tgaataccgc cgcaagagcg agcaaacggt
     1381 ggcgttttca cacaaagata agctcaaaac taaatggggc gattttaccg cgcattattt
     1441 taattcagcg atcgacgcca acgtgcacgt tgcgctctcg cgcggtaaaa ttgacggcaa
     1501 agaacctgtg ctcgtacgcg tgcaccgtga agatgtgctt gccgatgttt ttcaaataac
     1561 accacgcaaa aattatatgg cggtcgacga agcgatggct gcattggcaa aggctaaacg
     1621 cggcgtgttt cttttcttgg gacaaaagtc gtcgcaactg atccccgaaa gcgacaggac
     1681 actacgcgag tacggtattg gcgcgcaaat tttaacacac ttgggcgtaa aaaaaattaa
     1741 acttttaacg cgtaacccgc gccgtgtggt gggactcgac gcgttcggtc ttgagatagt
     1801 agaaacggta accgagtgaa gcaggccgct gcgtacttgc ggatgatcaa attttcgcac
     1861 acgctttttg cgttaccctt tgctttggcc gcgctggtga ttatctatgc gcgctacaaa
     1921 agccaagttc atattacacc gcttaaagtg cttttgttaa ttctcgcgtt taccggcatg
     1981 cgtagttttg caatggcgat aaatcgtatt ttaga
//
LOCUS       DAOMPD010000493         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21729_length_2014_cov_3.579888, whole genome shotgun sequence.
ACCESSION   DAOMPD010000493 DAOMPD010000000
VERSION     DAOMPD010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21729_length_2014_cov_3.579888"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLY93_13270"
     CDS             complement(<1..317)
                     /locus_tag="PLY93_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRP70494.1"
                     /translation="MSNSNTQAAPHSHLKDLALTFLGLIVLTIITVKISKVDFGSVAI
                     NISIALFIATIKALMVALYFMHLKWEDKLIIGVALMSIPFLILMVSTMVWDVANKTAE
                     GAY"
     gene            complement(338..553)
                     /locus_tag="PLY93_13275"
                     /pseudo
     CDS             complement(338..553)
                     /locus_tag="PLY93_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
     gene            complement(752..1135)
                     /locus_tag="PLY93_13280"
                     /pseudo
     CDS             complement(752..1135)
                     /locus_tag="PLY93_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
     gene            complement(1150..>2014)
                     /locus_tag="PLY93_13285"
     CDS             complement(1150..>2014)
                     /locus_tag="PLY93_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRP70495.1"
                     /translation="VYLMIIPAMGVVSEIIPVFSREPIFGYKAIAWSSVAIAGVSFLV
                     WAHHIFVSTLSTSAAVFFSLMTYFVAIPTAIKVFNWITTLYGGQISLKTPMLYTLGFV
                     FLFAIGGLTGLPLGAPSVDMSYHDTYFVVAHFHYTIQGGTVIALMAALYYWMPKFSGR
                     MYNEKLGVLGFFLVFIGFNLTFIPQFMMGTQGMPRRYYDYEPIFHIYHMLSTFGAYTL
                     GIGFLVAISNIIYSCYRGEKAPDNPWGANTLEWKIPTPMPTHNFHEIPTVTEWPYEYG
                     RDGVPLAEQKK"
BASE COUNT          571 a          440 c          500 g          503 t
ORIGIN      
        1 tggtatgcgc cttcggcggt cttattggcg acatcccata ccatggttga gaccatgagg
       61 atgagaaacg gaatcgacat gagcgctacg ccgatgataa gtttatcttc ccatttgagg
      121 tgcataaaat agagggcgac catcaacgct tttatcgttg caataaaaag tgcaatagaa
      181 atgttgatgg cgaccgagcc aaaatccact ttggagattt ttaccgtaat gatcgttaag
      241 acaatgagtc ctaaaaatgt cagcgctaaa tctttgaggt gcgagtgtgg cgctgcttgg
      301 gtgtttgaat tagacattgt gttctccttg tattctttta tgcgaccaga tacagtaacg
      361 gaaataaaaa aatccagata aggtcgacta agtgccagta aagaccggtg atttcaacgg
      421 gggtgtacca acccgggtga aattctttgc ggcggccttt gatgaaaacc catgtcaaca
      481 aacaaaggcc gatgatgatg tgaatgccgt gaatgccggt cataataaaa tagaggaaaa
      541 agaagatact cgcaaacgcg ggctttgttg gatctttgct gccgatattc gccgtgttga
      601 aaggaactac tttacatttt tcaacttcga tgtcagcgcc atgcagctta tcaaaagtgt
      661 tttcacattt gttttctgct ttgagtgttt tgtagagatc gggcgcgatt gccgctttgg
      721 ctaattcgac ttcgtgcatt ttatgactat attcgaattt tttattcacg agaaagacga
      781 caccgcagac aatagtaatc agcaacagcg cggacatgag ccgtgtttgg ccgtgtttta
      841 ccgcttcaat cgcccacgca acagtgaacg agctaaagag gagaatgatg gtgttggctg
      901 tgccaaacgc cacgctcaat tcttttgccg atgcgtggaa tcctgcgggg tcgcggtagt
      961 agccaatcgc atacgcggtg aataaaattg caaagagaag aatctctgta cctaaaaata
     1021 accacatgcc gaatttcgac atcgaataca cgacggatgc cgggtttccc caaaggctac
     1081 cgtcgcggac ggaacggcct tcataggtgg tatagcctgc ctgatttgaa ctcatgtttt
     1141 ttagctccgt tatttttttt gttccgctaa aggaacacca tcgcggccat attcgtaagg
     1201 ccattcggtg acagtgggta tttcatgaaa gttgtgcgtg ggcatcggcg tggggatttt
     1261 ccactcgagc gtgtttgcgc cccatggatt atcgggcgcc ttttcgccac ggtagcacga
     1321 atagatgatg tttgaaatcg cgaccaagaa accaatacct aaggtgtatg caccgaatgt
     1381 cgagagcatg tggtagatgt ggaaaatcgg ttcataatcg tagtagcggc gcggcatccc
     1441 ctgcgtaccc atcatgaatt gagggatgaa cgtcaggtta aacccaatga agaccaagaa
     1501 gaagccaagc actcccagtt tttcattgta catacgccct gaaaatttag gcatccaata
     1561 gtagagcgct gccatgaggg caatcactgt accaccttgg atggtataat gaaagtgggc
     1621 gaccacaaag taggtatcgt ggtagctcat atccaccgac ggcgctccca agggtaagcc
     1681 ggttaatccg ccgatggcaa acaaaaacac aaaacctaat gtgtagagca taggggtttt
     1741 gagggaaatt tgcccaccgt aaagcgtagt aatccagtta aaaactttaa tcgctgtggg
     1801 gattgccaca aaatacgtca tgagtgaaaa gaacaccgct gccgaggtgg ataatgtgct
     1861 cacaaaaatg tggtgtgccc acactaaaaa gctcactcct gcaatcgcca ccgacgacca
     1921 cgcaatcgct ttgtagccaa aaataggttc gcgcgaaaag acggggataa tttcggaaac
     1981 gactcccatc gcaggaataa tcatgagata cacc
//
LOCUS       DAOMPD010000494         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21736_length_2014_cov_2.457887, whole genome shotgun sequence.
ACCESSION   DAOMPD010000494 DAOMPD010000000
VERSION     DAOMPD010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_2014_cov_2.457887"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /gene="dnaK"
                     /locus_tag="PLY93_13290"
     CDS             complement(<1..1618)
                     /gene="dnaK"
                     /locus_tag="PLY93_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRP70496.1"
                     /translation="MAKEKIIGIDLGTTNSCVSVMEGGEAVVIPNSEGARTTPSVVAF
                     TDKGETLTGQVAKNQMVTNPENTLRSVKRFIGRRFNEVSEDVRKVAYKVKEGASKDVL
                     IQTREKDYRPEEISARVLTKMKQTAEEFLGTKVEKAVVTVPAYFNDEQRQATKDAGRI
                     AGLDVVRIINEPTAAALAYGLDRDKSGEVIAVYDLGGGTFDISILELADNVFEVKATS
                     GDTQLGGDDFDQAVIDWIVAEFKKETAIDVSKDKMALQRLQEAAEKAKIELSNKETAD
                     INIPFITMDASGPRHLNMTLTRAKFNQLTAELVERSVGPCKKCIADSGYSIADINKVV
                     LVGGSTRIPAVREKVKEIFGKEPDKSVNPDEAVAIGAAIQGGVLAGEVTDVLLLDVTP
                     LSLGIETLGSVMTKIIARNTTIPTKKSEVFSTAADNQTAVDIHVLQGERELARDNRTL
                     GRFQLSEIPAAPRGTPQIEVTFDIDANGIVHVSAKDLKTGKEQKIKIEASSSLSESEI
                     EKMVKDAELNAEADKKAKEAASQYNELDHIV"
     gene            complement(1643..>2014)
                     /locus_tag="PLY93_13295"
     CDS             complement(1643..>2014)
                     /locus_tag="PLY93_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRP70497.1"
                     /translation="LPALDAFEQFFTAYEKKALESADIKAIVEGVQLVQKQILRVFNE
                     AGVEEVNPTGEEFNPNFMEALTLVEGDVEHETVMQVFQKGYRIQGRVIRPARVVVAKP
                     KTPAQKTTTENTEQQTGEESN"
BASE COUNT          388 a          508 c          509 g          609 t
ORIGIN      
        1 acacgatgtg gtcgagctcg ttatactgcg acgcggcttc tttggctttt ttatccgctt
       61 cagcgttgag ttcagcgtct ttcaccattt tttcgatttc ggactctgaa agcgagctag
      121 acgcttcaat tttaattttc tgctctttgc ctgtcttcaa gtccttcgca gaaacgtgga
      181 cgataccgtt ggcgtcgatg tcgaaagtga cttcgatttg cggtgttcct cgcggcgctg
      241 cggggatttc agaaagctga aagcggccaa gagtgcggtt gtcgcgcgct aattcgcgtt
      301 ccccttgcag gacgtggata tccaccgctg tttgattgtc ggctgcggta gaaaaaacct
      361 cggacttctt agttggtatc gttgtgttgc gggcgatgat tttggtcatc acactaccca
      421 aagtttcgat acccaaagac agcggtgtca cgtcgaggag gagtacgtcg gtcacttcac
      481 cggcaagaac accgccttgg atcgctgcgc caatggccac tgcttcgtcg gggttcactg
      541 atttgtcggg ttcttttcca aagatctctt ttactttttc gcgcacggcg gggatacggg
      601 tagaaccacc aacaagaacc actttgttga tgtcggcgat actgtaaccc gaatcggcga
      661 tacatttttt gcatggccca acagagcgtt cgacgagttc ggcagtgagt tggttgaact
      721 ttgcacgggt gagcgtcatg ttgaggtggc gcggcccaga ggcgtccatc gtgatgaatg
      781 ggatgttgat atcggcggtt tctttattgg aaagttcaat tttcgctttt tcagccgctt
      841 cttgcaaacg ctgcaacgcc attttatctt tgctcacgtc aattgctgtt tcttttttga
      901 actctgcaac gatccaatcg atgacggctt ggtcgaagtc gtcgccaccc aactgcgtgt
      961 cgccgctagt ggctttcacc tcaaagacat tgtctgccag ttccagaatc gaaatatcga
     1021 atgtgccgcc gcccaagtcg tacacggcga tgacttcgcc cgatttatcg cggtcaagac
     1081 cgtatgcgag agccgccgct gtgggttcgt tgatgatacg caccacgtcg agaccggcga
     1141 tgcggccagc atctttggtc gcttggcgtt gttcgtcgtt gaagtatgca ggcactgtta
     1201 cgaccgcttt ttcgacctta gttcccaaaa actcttcggc ggtttgtttc attttggtga
     1261 gcacgcgcgc tgaaatttct tcgggacgat aatccttttc acgggtttgg ataagtacat
     1321 ctttcgaggc accttcttta accttatacg ccactttgcg cacgtcttcg ctcacttcat
     1381 tgaagcggcg tccaataaag cgcttcactg aacgcagcgt gttctccggg ttagtaacca
     1441 tttggttttt tgccacttga ccggtgagcg tctccccttt atcggtgaat gcaaccaccg
     1501 atggcgtggt acgcgcacct tcgctgttgg ggataacgac cgcttcgccc ccttccataa
     1561 ccgaaacgca tgagttggtt gttcccaagt cgatgccaat aattttttct tttgccatgt
     1621 taatctcctt tagtaggtat tgttaattac tctcttctcc tgtttgttgt tctgtgtttt
     1681 ccgttgttgt tttttgtgcg ggtgttttag gttttgctac caccacccgt gcagggcgta
     1741 taacacgccc ctgaatacga tagccttttt ggaatacttg catcactgtt tcgtgctcaa
     1801 catcgccttc gacgagggtg agcgcttcca tgaagttggg gttaaattct tcgccggtgg
     1861 gatttacctc ttcgacgccc gcttcgttaa agacgcgcag gatttgcttt tgaacaagtt
     1921 gcacgccttc gacaatcgct ttgatgtcgg cgctctcgag cgcctttttt tcgtacgcag
     1981 taaaaaactg ttcaaatgca tcgagtgcgg gtaa
//
LOCUS       DAOMPD010000495         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_21760_length_2012_cov_2.671436, whole genome shotgun sequence.
ACCESSION   DAOMPD010000495 DAOMPD010000000
VERSION     DAOMPD010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21760_length_2012_cov_2.671436"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLY93_13300"
     CDS             complement(<1..481)
                     /locus_tag="PLY93_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70498.1"
                     /translation="MLVFFISISLYGTQPLLFKINPAAQTNTNTVCLSELVDTTSLPA
                     QVIEKLERYCKIETKGKTTRINAKDVELYAWAAGVVPDKIVGGSSLVEKTDIPLSRKN
                     PTQNARVKLRRGTQVRLSLTSPFMKIERDAQTLADAFTGETVDVRPTGTRKTLRARLI
                     "
     gene            complement(575..1342)
                     /gene="flgG"
                     /locus_tag="PLY93_13305"
     CDS             complement(575..1342)
                     /gene="flgG"
                     /locus_tag="PLY93_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRP70499.1"
                     /translation="MIAQQFNMDTISHNLSNVNTTGYKKNRVDFEDLLYQHQVLAGTP
                     STAVSEIPVGVNVGVGVRPAATQKLFQMGSLQSTGHKLDMAISSDVGFFKILMPDGTF
                     GYQRNGDFKIDSRRQVLQSDGYTLEPPLVIPPDGLIETLTINEQGEVRIQIGDDQIPR
                     QIGQIELYRFVNPAGLKAVGKNLFKESPASGPEIAGTPGLNGFGGILQGFLEMSNVNL
                     AEEMVNMIVAQRAYEANSKSIQTSDSMLSTAIQLKRG"
     assembly_gap    1345..1354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1467..>2012)
                     /locus_tag="PLY93_13310"
     CDS             complement(1467..>2012)
                     /locus_tag="PLY93_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRP70500.1"
                     /translation="MTSIPVLGVIDPAVSALTQKAKRQSAAGLIATKSTIHSNAYDAA
                     LKRHHSEHFLVSRACPLLVPIIEEGFIDTPITEEILRIYLQPMVEQGIEYLLLGCTHY
                     PLIARVITRLYPHLKLIDSAEETATTLKNLLTTKHLLNTASEGRIELFVSDMTESMRD
                     LKELFFQNSVDRFEVVKLTNE"
BASE COUNT          464 a          453 c          529 g          556 t
ORIGIN      
        1 cgataagacg cgcacgcaat gtcttgcgtg ttcccgttgg tcgcacgtca accgtttcac
       61 cggtgaacgc gtcggcgagc gtttgcgcgt cgcgctcaat tttcataaac ggcgatgtca
      121 aactcaagcg cacctgcgta ccacgacgca atttaacgcg cgcattttgt gtggggtttt
      181 tacgggaaag agggatgtcg gttttttcta cgagactgct gcctcccaca attttatcgg
      241 ggacaactcc cgctgcccac gcgtagagtt ccacgtcttt tgcgttaatg cgcgttgtct
      301 tgccttttgt ttctatttta caataacgct ccaatttttc gatgacctgc gcaggtaaag
      361 aagtcgtatc taccaattcg ctgaggcaca cggtgttggt gttcgtctgc gccgcaggat
      421 tgattttgaa taacagaggt tgggttccat aaagggagat agatatgaaa aataccagca
      481 tcgcaaagac gcatttcaat gcgtctttgc ggtaccatga cggatcccta cgaatacgaa
      541 aattaaattt gcgtaacgtc aatccatccg cctattaacc gcgtttcagt tgtatcgccg
      601 tcgataacat gctgtcggat gtttgaatcg attttgagtt ggcctcgtac gcgcgttgcg
      661 cgacgatcat attcaccatc tcttcggcga gattcacgtt cgacatctcc aaaaaacctt
      721 gaaggatgcc gccaaatcca ttgaggccgg gcgtgcctgc aatttctggt cctgaggctg
      781 gtgattcttt gaaaagattt ttgccgactg ctttaagtcc ggcggggttt acaaaacgat
      841 agagttcaat ttgaccaatt tggcggggga tttggtcgtc gccaatttgg atgcgcactt
      901 ctccctgttc gttgatggtg agagtttcaa tgaggccatc gggggggatt accaacgggg
      961 gctctaaagt gtaaccgtcg gattgcaaca cctggcggcg tgaatcgatt ttgaaatcgc
     1021 cgttgcgctg gtaaccgaac gtgccatcgg gcattagaat tttaaaaaaa cccacgtcgg
     1081 aggaaatcgc catatcaagt ttgtggccgg tcgattgtag cgaacccatt tggaaaagct
     1141 tttgcgtcgc agcgggacgc acacccacac ccacgttcac acccaccggg atttcagaaa
     1201 ccgccgtcga gggagtccct gcgagcactt ggtgttggta gagtaaatct tcaaagtcca
     1261 cgcggttttt cttgtaaccg gtggtgttca cgttcgacaa gttgtgagaa attgtgtcca
     1321 tgttaaattg ttgcgcgatc atgcnnnnnn nnnnagagcg taccatactt tatattcggt
     1381 tgctttttgg aatcggggga gaaaaatttg tgacagggat agggcgctac gcgagttggc
     1441 gtacaggctc gtaggtttat ccgcttttac tcattcgtga gtttgaccac ttcaaagcga
     1501 tcaacgctgt tttgaaaaaa gagttctttt aaatcacgca tgctctccgt catgtcggaa
     1561 acaaaaagtt caatccggcc ttcggaggca gtgttgagga gatgctttgt cgttaataaa
     1621 tttttgagcg tcgtcgctgt ctcctcggcg ctatcgatga gttttaagtg tggatagagc
     1681 cgtgtaatta cgcgagcaat taaaggatag tgggtgcatc ccaataaaag atattcgatg
     1741 ccttgttcga ccatcggctg taaataaata cgtaaaattt cttcggtgat gggggtgtcg
     1801 ataaagcctt cttcgatgat gggaaccaag agcgggcatg cgcggctcac cagaaaatgt
     1861 tcgctatggt gtcgtttgag tgcagcatcg taagcattgc tgtggatggt gctcttggtt
     1921 gcaatgagcc ccgcagcgct ttgtcttttt gccttttgtg tgagagcgct caccgcaggg
     1981 tcaattacgc ccaagacggg aatggaggtc at
//
LOCUS       DAOMPD010000496         1994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22014_length_1994_cov_1.680248, whole genome shotgun sequence.
ACCESSION   DAOMPD010000496 DAOMPD010000000
VERSION     DAOMPD010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22014_length_1994_cov_1.680248"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..>1994
                     /gene="mnmG"
                     /locus_tag="PLY93_13315"
     CDS             210..>1994
                     /gene="mnmG"
                     /locus_tag="PLY93_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRP70501.1"
                     /translation="MQVSDTRFKYPRTYDAIVVGGGHAGCDAAHVAAKAGLKTLLVTM
                     ALDTIGQMSCNPSIGGVAKGHIVREIDALGGLMGVATDRTGIHYKMLNRSKGPAVWGP
                     RAQADKKLDQNEVKYILESTPNLDILQDAVADILTDAVGQLPHVSGIKTQRGLEIFAP
                     AVVITTGTFLRGLIHVGDFQTQAGRYGDKSSQELSPSLMRIGFELGRLKTGTPPRLHG
                     DSIDYSVMQLQHPDENPQPFSYAFEYAQEKLWQTQIDCHITHTSATTHDIIRGALDRS
                     PLYSGKIKSIGPRYCPSIEDKVVRFAEKERHQIFLEREGLRTREVYANGISTSLPEDV
                     QWNIVRSIKGLEDAVIMRPGYAVEYDFIQPTELYPTLETKKVRGLYLAGQINGTTGYE
                     EAAAQGLIAAYNIIYRAQGRGEFILRRDEAYIGVLIDDLVNKGVDEPYRMFTSRAEHR
                     LTLRQDNADLRLMHYAAALTLDGGRNDFCKEKYARYAHYKTYIATHKASHNLIQQLST
                     MNTLVQKGTTYEAALRRPQLSDEAIRLVFVDVVASQETPALPPLTDEEVFKVAMEIKY
                     DGYGAREANRIERRLGSEERPIPDDFDYD"
BASE COUNT          515 a          524 c          517 g          438 t
ORIGIN      
        1 gttatgcacc acggtacggt tgccgtacac tttcactaaa tttttggttt ccagagtgag
       61 cgcatcgggc ttgataaccg gcgcgggttt atctaaaatg ctttttgtcc gcgtcgcgcg
      121 cgccatagtt tatttcgagt gtctgtgctg tggcggcaag ctcttatgag atttctttac
      181 gcgctgtcaa aactatcgtc tgtggaggtg tgcaagtgag cgacacgcgc ttcaagtatc
      241 cgcgcacata cgatgcgatt gttgtcggtg gcggccacgc gggctgcgat gcggcgcacg
      301 ttgctgccaa ggcggggctt aaaacgttgc tcgtcaccat ggcgctcgac accatcggcc
      361 aaatgagttg taacccgtcg attggcggtg tcgcgaaagg acacatcgtg cgcgaaatag
      421 acgcgttggg tggactcatg ggcgtcgcca ccgaccgtac tggcatccat tacaaaatgc
      481 tcaaccgctc caaagggcct gccgtgtggg gaccgcgtgc acaagcagat aaaaagctgg
      541 accaaaatga agtgaagtac attcttgaat cgacaccgaa tctcgatata cttcaagacg
      601 cggtggcaga tattttaacc gatgccgtag ggcaattacc ccatgtcagt ggaataaaaa
      661 cccaacgagg attagaaatt tttgcacccg ctgtggtcat caccaccgga acgtttttgc
      721 gtggcctcat ccatgtcggt gattttcaaa cgcaggcggg acgctatggc gataaaagtt
      781 cgcaagagct ttccccctcg ctcatgcgta ttgggtttga gttgggcagg cttaaaaccg
      841 gcacaccacc gcgcctccac ggcgatagta tcgattactc ggtgatgcaa ctccaacacc
      901 ccgacgaaaa cccacaaccg tttagttatg cgtttgaata cgcccaggaa aaattatggc
      961 aaacccaaat cgattgccac atcacgcata catctgccac aacgcatgat atcatccgtg
     1021 gcgcgttgga cagatcgccc ttgtattcgg gaaaaataaa atcgattggg ccgcgctact
     1081 gtccttcgat tgaagataaa gtggtgcgct ttgccgagaa agaacgccac caaatttttt
     1141 tagagcgcga gggattacgc acacgcgagg tgtacgcaaa cggtatctcg acaagcttgc
     1201 ccgaagacgt gcagtggaac atcgtacgct cgatcaaagg gttggaggat gcagtcatca
     1261 tgcggccagg gtatgccgtc gaatacgatt ttattcaacc caccgaactt tatcccacac
     1321 tcgaaaccaa aaaagtcaga gggctctatc tcgccggcca aatcaatggc accacaggct
     1381 acgaagaagc agcggcgcag ggacttatag ccgcctacaa tattatttac cgtgcacaag
     1441 gtcgaggcga atttattttg agacgcgacg aagcgtatat cggcgttttg attgacgact
     1501 tggtgaacaa aggcgtcgat gaaccatacc gcatgttcac ctcgcgcgcc gagcaccgcc
     1561 taacgctgcg ccaagacaac gccgatttac gtctcatgca ctacgctgcg gcactgactt
     1621 tggatggcgg ccggaacgat ttttgcaaag aaaaatatgc acgctacgct cactataaaa
     1681 catacatcgc cacgcacaaa gccagccaca acctcatcca acagttgagt acgatgaaca
     1741 cgttggtgca aaaaggcacc acgtatgaag cggcactcag aagacctcaa ctgagcgatg
     1801 aggccatccg tctggtgttt gtcgacgttg tcgcatcgca agagacaccc gcgttgccgc
     1861 cgctcaccga cgaagaagtt ttcaaagtgg cgatggagat taaatacgat gggtatggcg
     1921 cgcgcgaagc caatcgtatc gagcgccgat tgggttctga agaacgcccc atcccagacg
     1981 attttgatta tgac
//
LOCUS       DAOMPD010000497         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22118_length_1986_cov_2.394096, whole genome shotgun sequence.
ACCESSION   DAOMPD010000497 DAOMPD010000000
VERSION     DAOMPD010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22118_length_1986_cov_2.394096"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..883
                     /locus_tag="PLY93_13320"
     CDS             107..883
                     /locus_tag="PLY93_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70502.1"
                     /translation="MDYFENKITLLTGASGGLGSEFATQLLDLGAHMILSDLKIESLD
                     RYKSYSGKGKVLGLIAGDVSTKEGTERFLADVKKVTSCPDILINNAGIAQVGPFGEIP
                     LDRWEKLMNINLISPMRICHYFLPVMAERGSGHIVNISSVAGIVPMPGGAPYTASKHG
                     IKGFSDAMRIDYAKKGILVSTVHPFFTKTAILQSERFGGVEVNADDYSFMIETPEKVI
                     RETIKAIANKSEFILPGPTAKLLDLIRRFAPSLIGITKFF"
     gene            921..1817
                     /locus_tag="PLY93_13325"
     CDS             921..1817
                     /locus_tag="PLY93_13325"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HRP70503.1"
                     /translation="MRAKSAINCVSIAVGFIAVFTFIVNAAAFSAQEPVRVFGEYGKL
                     QTLAVSPNGKFLAFSSDYRDQKGDIILYNIETKEKQTITKTPYLETQPVFSRDGRSVY
                     YFINEAGAGSIKKINLADGSVALVTDRNFWCEFPTLSPDGHSLAYYSKRNNRYNLYEM
                     NLSTGKEIQLTNEQHFDFGPVYSANGRNIYFYSNRKGSSFSLFRYERASQKITPLYGP
                     GGFTFQPTSDVHGNLIFAVSNVSGNNDIYAISLNANRVDPVTNSPGNDLFPAVDARHS
                     QLYYISQKDGDYAVYKRQFTAN"
     gene            1836..>1986
                     /locus_tag="PLY93_13330"
     CDS             1836..>1986
                     /locus_tag="PLY93_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70504.1"
                     /translation="MSTQKEYTAEKIHPTAFVHENATLYGDITLGEYASIWPGVVLRA
                     DMNAFV"
BASE COUNT          530 a          496 c          447 g          513 t
ORIGIN      
        1 taaaacgcac tcaaattgac cccaaaagtg tcagttatct ctgtgggttg cataggctct
       61 tatagattct taattgacac aatgttcaat ttctacgtgc tgcggcatgg actattttga
      121 aaataaaata accttgctca ccggtgcgtc gggcggtttg ggcagcgagt ttgcaacaca
      181 gttactcgat ttaggcgcgc acatgatttt atccgacttg aaaatcgaat cgctcgatcg
      241 ctataaatcg tactcgggca aaggcaaagt gcttggactc atcgcgggtg atgtttctac
      301 taaagaaggt acggagcgtt ttttagccga tgtgaaaaaa gtcacctcat gccccgacat
      361 tcttatcaac aacgctggca ttgctcaagt ggggccattc ggggaaatcc ccctcgaccg
      421 ttgggaaaaa ctcatgaata ttaatctcat atcgccgatg cgcatctgcc attatttttt
      481 acctgtcatg gccgaacgag gcagcggcca catcgtgaac atttcatcgg tggcgggcat
      541 tgtgccgatg ccaggcggtg cgccttacac cgcgtccaaa cacggcatta aaggcttttc
      601 agacgcgatg cggattgact acgctaaaaa aggaattctt gtatccacgg ttcatccctt
      661 tttcaccaag acggcgattt tgcaatcgga gcggtttggc ggtgtcgagg tgaacgccga
      721 cgattactcg tttatgattg aaacacccga gaaagtcatc cgcgagacga ttaaagcaat
      781 cgccaacaaa agtgaattta ttttgccagg ccccacggca aagctgctcg atttaatccg
      841 tcgttttgca ccgagtctca ttggtataac taaatttttt taggtgcttg ctctcccgtg
      901 cccatgattt aacatggtgc atgcgtgcaa aaagcgcaat caactgtgtg tcaatagccg
      961 tgggctttat cgcggtattt actttcatcg taaatgccgc agcgttttca gcgcaagagc
     1021 ctgtgcgggt gtttggcgaa tatggcaaac tacaaacgct cgccgtttcg cccaacggca
     1081 agtttttagc gtttagttca gattaccgcg accaaaaagg cgacattatt ctctacaata
     1141 tcgagaccaa agaaaagcaa acgattacca aaactcccta ccttgaaacg caaccggtct
     1201 tttcgcgcga cgggcgcagc gtctattact tcatcaacga agcgggtgcg ggttccatta
     1261 aaaaaataaa tcttgccgac ggcagtgtcg ctctcgtcac cgaccgcaat ttctggtgcg
     1321 agttcccaac actgtccccc gacggtcatt cgttggcgta ttattccaag cgcaataacc
     1381 gctacaatct gtacgaaatg aatttgtcga ccggcaagga aatacagctc accaatgaac
     1441 aacattttga tttcgggcct gtctattcag ccaatggccg caatatttat ttttactcaa
     1501 atcgcaaagg aagctcattt tctcttttcc gctacgaacg tgcatcgcaa aaaataacgc
     1561 ccctctatgg tccgggcggg tttactttcc aaccgacgag cgacgtgcac ggaaacctca
     1621 tttttgccgt atcgaatgtt tcgggcaaca acgatattta tgccatttct ctgaacgcaa
     1681 accgtgtgga ccctgtgact aattctcctg gaaacgatct tttccccgca gtggatgctc
     1741 ggcacagcca actctattac atcagccaaa aagacggaga ctacgccgtt tacaaaaggc
     1801 aattcactgc aaactgagta cgggaaaaca caatcgtgtc gacacaaaaa gaatacactg
     1861 ctgaaaaaat acatcccaca gcgtttgttc atgaaaatgc aactctctat ggcgacatca
     1921 ctttaggcga atacgcgtcg atttggccgg gggtggtatt acgcgccgac atgaacgcgt
     1981 ttgtgt
//
LOCUS       DAOMPD010000498         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22281_length_1975_cov_2.269271, whole genome shotgun sequence.
ACCESSION   DAOMPD010000498 DAOMPD010000000
VERSION     DAOMPD010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22281_length_1975_cov_2.269271"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLY93_13335"
     CDS             <1..921
                     /locus_tag="PLY93_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRP70505.1"
                     /translation="KRALTIAAVGRHHLLMVGSPGTGKSLLARAYRYILPPLSFSEAA
                     EVTRVHSLAGFSNGDLVRSRPFRSPHHSTSMPALVGGSSLIKPGEISLAHLGTLFLDE
                     LPEFRNEALQSLREPLEEKKITIARARGHITYPADFQLIAAANPCRCGELFNQSGKCE
                     CRHGNALSQFSKLVGPFLDRIAIELNLNELPQHLPATPITTTEKARTSISSARKYSQE
                     RNGKGILNSQFSVEKLYRLFEKIKPMRIYERYAQIEHLTMRSWVNTLRVALSIADLER
                     TTPNEAHIMEAMQYRFLRKRLDALRSLKAA"
     gene            complement(966..1859)
                     /gene="xerD"
                     /locus_tag="PLY93_13340"
     CDS             complement(966..1859)
                     /gene="xerD"
                     /locus_tag="PLY93_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRP70506.1"
                     /translation="MAVRRNYITNFKEYLSLERGLSQNSIFSYVSDVQKLEKFLTKEK
                     KQLQSSSQKDISNFLREESRKKLSSRTRARVVASLRQFYGYLENRKFIETNPMETIEA
                     PKIEKALPDFLTQDEIRKLFAAFNDKNLLELRDKTMFEFLYSAGLRISEACMLLLSDI
                     DRENQVLTIKGKGDRERLVPYGEVAAELLNTYLDSARSDILNQYESNYVFVSKKGGAL
                     SRKSAWRLLKRYMKRAGIGRNITPHTLRHSFATHLLQNDADLRAVQELLGHMDIATTQ
                     IYTHLASNELKKTHQTYHNRA"
BASE COUNT          492 a          485 c          460 g          538 t
ORIGIN      
        1 aagcgtgcgc tcacgattgc tgcggtgggg cgtcatcatt tactcatggt gggctctcct
       61 ggtacaggga aaagtttact cgcgcgcgcg taccgctata ttttaccgcc gctttctttc
      121 agcgaagcag ccgaggtgac gcgcgtacat tcgctcgccg gattctcgaa cggtgatttg
      181 gtgcgttcac gtcctttccg ttcgccgcac cacagcacgt cgatgccggc gttggtgggc
      241 ggtagcagtt taattaaacc tggagaaatt agccttgcgc acttaggtac actttttctc
      301 gacgaactcc ccgaatttcg taacgaagcg ctccaaagcc tacgcgagcc tcttgaagaa
      361 aaaaagatta ccatcgcgcg agcgcgcggg catatcacct accccgctga tttccagctc
      421 atcgccgcag cgaacccttg ccgttgcggc gagctcttta accaaagcgg caaatgcgag
      481 tgccgccatg gcaatgctct cagtcagttt tcaaaactgg ttggcccttt tttagaccgc
      541 atcgccatag aattaaattt gaacgaactg ccacaacatc taccggctac tcccatcacc
      601 accaccgaaa aagcacggac tagcatttcc tctgcacgaa aatatagcca ggagcgtaac
      661 ggcaaaggta ttctcaacag ccagtttagc gtagaaaagc tttatcgcct ttttgaaaaa
      721 ataaagccga tgcgtattta cgaaaggtat gcacaaatag aacatctcac gatgcgttca
      781 tgggtcaata cattgcgcgt tgcactcagt attgccgatt tagaaagaac cacacctaat
      841 gaggcacaca ttatggaagc gatgcaatac cgctttttac gcaagcggct tgacgcacta
      901 cgctccttaa aggcagcata aacaatagta ttttttgctt ttagcttaaa aaataataaa
      961 aggttttatg cccggttgtg gtaagtctgg tgtgtttttt ttaattcgtt gctcgctaag
     1021 tgggtgtaaa tctgcgtcgt ggcaatatcc atgtggccta aaagctcttg cactgcgcgc
     1081 aagtcggcgt cgttttgcaa taggtgcgtc gcgaacgagt gacgcagcgt gtgcggagtg
     1141 atgttgcgcc caatacccgc acgtttcata taacgcttca aaagccgcca tgcgcttttg
     1201 cgcgagagtg cgccgccttt tttagaaaca aagacataat tcgattcgta ttgatttaag
     1261 atatcactgc gtgcgctgtc gagataagtg ttgagcaatt ctgcagccac ttcaccataa
     1321 ggcaccaacc gctctctgtc gcccttccct ttgatcgtca gcacttggtt ttcacggtcg
     1381 atgtccgaaa gaagcaacat gcacgcctct gaaatgcgca agcctgccga ataaagaaat
     1441 tcaaacatcg ttttgtcgcg caattcgagt aaatttttat cgttgaacgc tgcgaagagc
     1501 ttacggattt catcttgcgt cagaaaatcg ggcagcgctt tttcaatttt aggcgcttcg
     1561 atggtttcca tgggatttgt ctcgataaat ttgcggtttt caagataccc ataaaactgg
     1621 cgcagcgagg ccaccacgcg cgcacgggtg cgactcgaca actttttgcg cgattcttca
     1681 cgcagaaaat tgctgatgtc tttttgcgaa ctcgactgga gttgcttttt ttctttggtt
     1741 aaaaattttt ccagcttttg cacgtcggag acatacgaaa aaatactgtt ttgagaaagc
     1801 ccacgttcga ggcttaaata ctctttgaag ttggtgatgt agttgcgtcg tacagccatt
     1861 atatttcatt ttcgacaaag gttattttta ttttgacaca ttttttatgt cacacgaaac
     1921 taacagggtg ttgaaaaatg ccgtgcgcgc cgcagacgcc ggggttgccc ttagc
//
LOCUS       DAOMPD010000499         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22300_length_1973_cov_3.340459, whole genome shotgun sequence.
ACCESSION   DAOMPD010000499 DAOMPD010000000
VERSION     DAOMPD010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22300_length_1973_cov_3.340459"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>1973
                     /locus_tag="PLY93_13345"
     CDS             139..>1973
                     /locus_tag="PLY93_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP70507.1"
                     /translation="MSELPMNLDSKVNVQLDQSYEPGPGFLFIPPISKARYDFILQVQ
                     SAFVNKVVSFTFPDFSHCFEYSLFEIIDGENLRVSASSSQFYLQELAETKNIDGFKKF
                     IARTRPLAIQHEKNLSAQMMRQSLYNVAHNLPPMVEFLHIPSEARLHSAMKRLAPKGM
                     RYSVGIPIIIDRKPIGVLWGIHRKSFNGEERRDVRQQMISLSRAIDFVISEEWAETSD
                     AFSTRRKIEKHDPTANLESLVYTQQALQEAPIKSMVLYSNRYAEKYRQDTNYIVETVN
                     GYKISLKRYLPEHLNGQDRILLMLPGFFCNRSIMDRLAKEMALEHGYPVFTLDIRGRS
                     TETLPQKRFNNNHWSVDNYIWEDFPAALHWLKETHPGKKVVVYGHSMGGMIPLFYSGA
                     YDRYGKSQARNLAIKPESIIDGIVTITSPIYVRIAAQSTWFNMFRNSAKLLTNNVFAD
                     SIMKLLDYTLATTVGGIDLNKFFTFLNSISASVRTLSFDVTYRLPTIKEFVGYEQITP
                     PEWYFLMEDVFCEESTLVISQFIRSLIDGDNFTSIDKKINYTLELNNLEIPHFTVVGS
                     IDEIAPPDTVRQGHLAGKSPKNKIAEYKQGHLGIITHPETVKQIAV"
BASE COUNT          553 a          502 c          408 g          510 t
ORIGIN      
        1 ctctaaaatc aaaaatttaa cttacgctac gctaagttat acgaagttaa gctcgttttt
       61 agcgttaaaa aatgccaaaa aatgagttgg cgcacaggct cctaaccaga tagactctta
      121 cttgtcgcgc tatttaatgt gtcagaattg ccaatgaatt tggatagcaa agtcaacgtc
      181 caactggatc aatcgtatga gccgggtccg ggatttcttt ttataccccc catctctaaa
      241 gcgcggtatg attttattct acaagtccag agcgctttcg taaacaaagt cgtttcattt
      301 acttttcccg atttttcaca ctgttttgaa tatagcttgt ttgaaatcat cgacggcgaa
      361 aatttacgcg tctcggcgtc ttcatcgcaa ttttacttac aagaactcgc tgagacaaaa
      421 aatatcgacg gtttcaaaaa atttatcgcc cgcacacgcc cactggccat ccagcatgaa
      481 aaaaatctct cggcgcagat gatgcgccaa tcgctttaca acgtcgcgca caatttgccg
      541 ccgatggttg aattcttgca tatcccttct gaagcgcggt tgcactcggc aatgaaacgt
      601 cttgccccca aagggatgcg ttactcggtg ggaatcccca tcatcatcga cagaaaaccc
      661 attggcgttt tgtggggtat ccaccgcaaa tcgtttaacg gcgaagaaag gcgcgatgtg
      721 cgccagcaaa tgatttcact cagccgtgcg attgactttg ttatctcaga ggaatgggcg
      781 gagacctctg acgcatttag cacgcgccgc aaaattgaaa aacacgaccc aaccgcgaat
      841 ttagaaagtc tggtctatac acaacaagcg ttacaagagg cgccgattaa atcgatggtg
      901 ctttattcaa accggtatgc tgaaaaatac cgccaagaca ccaactacat cgtcgaaacc
      961 gtcaatggat ataaaattag cttaaagcgt tatttgcctg agcacctcaa cggccaagac
     1021 cgtattttgc tcatgctccc cggctttttt tgtaaccgtt ccattatgga tcgcctcgcc
     1081 aaagaaatgg cgttagaaca tggctatccc gttttcactc tggatataag aggccgctca
     1141 acggagacat tgccccaaaa acgatttaat aataaccact ggtcggtgga caattacatt
     1201 tgggaagact ttcccgctgc gctccattgg ctaaaagaaa cgcacccggg aaaaaaagta
     1261 gtcgtgtacg gccacagcat gggcggtatg attcccctct tctattcggg agcgtacgac
     1321 cgctacggca aaagccaggc gcgcaactta gccatcaaac ccgaatcgat catcgacggc
     1381 atcgtcacca tcactagccc catctatgtg cgcatcgccg cacagtcgac gtggttcaat
     1441 atgtttcgta actcggcaaa actccttaca aacaacgttt ttgccgattc cattatgaaa
     1501 ctcctcgact acacgttagc aacgaccgtc ggtgggattg acctcaataa attttttacg
     1561 ttcctcaata gcatctccgc ttcggtacgc acgctttctt tcgatgtgac ataccgatta
     1621 cccaccatca aagaatttgt gggctatgaa cagattactc ctcccgagtg gtatttcctt
     1681 atggaggatg tcttctgtga agaatcaacg cttgtgatta gccagtttat tcgttcgctc
     1741 atcgacggcg ataattttac ctcgattgac aaaaaaatta attatacgct tgaactcaac
     1801 aaccttgaaa ttcctcactt caccgttgtg ggttccatcg acgaaattgc tccccccgac
     1861 actgtgcggc aagggcattt ggcggggaaa tcgcccaaaa ataaaattgc cgagtacaag
     1921 caagggcatc tgggaattat cactcaccct gaaacggtga aacaaatcgc cgt
//
LOCUS       DAOMPD010000500         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22341_length_1970_cov_2.312794, whole genome shotgun sequence.
ACCESSION   DAOMPD010000500 DAOMPD010000000
VERSION     DAOMPD010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22341_length_1970_cov_2.312794"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..948)
                     /locus_tag="PLY93_13350"
     CDS             complement(229..948)
                     /locus_tag="PLY93_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70508.1"
                     /translation="MVEANIQCIALVGAGPSLDLCLDVMNTLVARGAFFCVADSVAVA
                     FQKKFPQTPFCVFTVETRRHAYLLRLKKNPAAPVTLWAYHNANARNLIKFHQGYTIQR
                     FKLVGEEGGLPTLYSPGTVFGVMLAFAMERIAPRGEIHLIGADFSYLDQRIYTRFLEP
                     HTPLTTRLFTREIWQLEMVFKKTSQVILKHGVVVRTSFEFLNARENIRAFVERLGEGV
                     RLVEYSPLGVDTAWVEKRAPF"
     gene            complement(935..>1970)
                     /locus_tag="PLY93_13355"
     CDS             complement(935..>1970)
                     /locus_tag="PLY93_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRP70509.1"
                     /translation="AGVFLLIRLYPFWYALPLVKIATGAIGGITLVTASLSAKAQSNI
                     KGQIGYASVAQVGVMFVELALGYRDIALFHFMGNAVLRCYQLLASPSVLVIFLRMQNE
                     GAVLSSGVTGFLARFAGIKFRRVLYAIAFSECYGETIVNFLLKTLKAFAQFASRVVSY
                     QFSILVLVLLLTIVILEKQFPAALAAQVIRLTFGFLAVYLALAAFGEKVSPVRLLNNA
                     SLSSLLTGLSTLFLAPEYFMHTALFFLGVGAGYFLARVALYLLPAEKLQLNYAGMGVI
                     FPNITLLLLAGILSLSGFTLTPTFLGEDLLLHYGAGKFVFETFIIAGTFILNAVTLMR
                     MYVKLSYGRG"
BASE COUNT          565 a          485 c          500 g          420 t
ORIGIN      
        1 tcgcaaaatt tagtcgatag cgtagggtat cggctcacgg taacgcttta tcgtttggac
       61 aattaagagc ctgtgcgtca actcgcgcag cgtgtaggaa aggcaatttt cttgcaaatt
      121 tttgtacgcg gttcgcttaa aaacgcgaaa ttttgcacaa attcgggcgt cctcgtcccg
      181 tgcagagcaa attggaacaa tttgccgccg gcctcacgac ggtgcgtttc aaaacggcgc
      241 gcgtttttcc acccacgctg tatccacgcc caggggtgaa tactcgacaa ggcggacccc
      301 ttctcccaag cgctcgacaa aggcacggat attttcgcgc gcatttaaaa attcaaacga
      361 agtgcgtacc acaacaccgt gcttcaagat cacttggctt gtttttttga aaaccatttc
      421 gagctgccat atctcgcgcg tgaaaaggcg cgttgtgagg ggcgtgtgcg gttcaagaaa
      481 gcgcgtgtag atgcgttgat ccagatatga aaaatcggcg ccaatgaggt ggatttctcc
      541 cctgggtgcg atgcgttcca ttgcaaacgc aagcatcacc ccaaagacgg tgccggggga
      601 ataaagcgtg ggcaacccgc cttcttcacc cacaagtttg aatctttgga tggtgtagcc
      661 ttgatggaat tttataagat tacgcgcgtt ggcgttgtgg tatgcccaaa gggtaactgg
      721 cgccgctgga ttttttttca agcgcaagag atacgcatgg cgtcgtgttt cgacggtgaa
      781 gacacaaaaa ggagtttggg gaaatttctt ttggaacgcc accgcgactg aatcggcgac
      841 gcaaaaaaaa gcgcctcgtg ctacaagagt attcatgaca tcgagacaca gatcaagcga
      901 tggccctgcg cccaccaggg cgatacattg aatgttagcc tctaccatac gacagcttta
      961 cgtacatccg catgagcgtc actgcgttta ggataaaagt gcctgcgata ataaacgttt
     1021 caaaaacaaa ttttcctgcg ccgtagtgga gtaacaaatc ctcgcccaaa aatgttggcg
     1081 tgagcgtaaa tccactgagc gacaaaatac cagcgagcag cagcaaagta atgttgggaa
     1141 aaataactcc catgcctgcg tagtttaatt gtaacttctc ggcgggtaaa aggtaaagag
     1201 ccacgcgggc gagaaaataa cccgcaccca cgccgagaaa aaaaagagcc gtgtgcatga
     1261 aatactcggg cgctaaaaaa agggtcgaga gccctgtgag gagagaagag agcgaagcgt
     1321 tgttgagtaa acgcacgggc gatacctttt cgccgaatgc cgcgagcgcc agatataccg
     1381 ccaaaaaacc aaacgtgagg cggatgactt gcgccgcgag cgccgcaggg aactgttttt
     1441 ctaagatcac gatggtgagt agaagtacta aaactaaaat gctaaattgg tacgacacca
     1501 cacggctggc aaattgtgca aacgctttga gtgttttcaa aagaaaattc acaatcgttt
     1561 cgccatagca ttctgaaaat gcaatagcat agagtacgcg tcgaaattta attccggcaa
     1621 agcgcgcaag aaaaccggta acgccgctgc ttaataccgc gccttcgttt tgcatccgca
     1681 aaaaaataac gagcaccgat ggggaagcca ggagctgata gcaccgcagc accgcgttac
     1741 ccatgaaatg aaaaagcgca atgtcgcggt aacccaacgc cagctcgacg aacatcaccc
     1801 ccacttgcgc caccgacgca taaccgattt ggcctttgat gttagattgc gctttagcgg
     1861 aaagcgacgc agtaacgagg gtgatcccgc ctatagcgcc cgttgcaatt ttcacaagcg
     1921 gcaacgcata ccaaaacgga tacaatcgaa tgagtaaaaa cacacccgcg
//
LOCUS       DAOMPD010000501         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22361_length_1969_cov_2.100313, whole genome shotgun sequence.
ACCESSION   DAOMPD010000501 DAOMPD010000000
VERSION     DAOMPD010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22361_length_1969_cov_2.100313"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>572)
                     /locus_tag="PLY93_13360"
     CDS             complement(<1..>572)
                     /locus_tag="PLY93_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70510.1"
                     /translation="THSTVDYGGGDDFDIEQLFEDGAKASPSAWSAPEEAIQQLTDGD
                     IEPSGGAYSSSESKVSYDITEIIFGESTVAESNNTIDAVPSTPSFAENLEFKIDTSEE
                     YEDETILPATEEKPRTIAVENIPKPREAKITPELKKPILVERASITPVLQVSRQRELL
                     FPMLKTQKRALEITNPAEAQQVIEKLEETL"
     assembly_gap    573..616
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<617..1051)
                     /locus_tag="PLY93_13365"
     CDS             complement(<617..1051)
                     /locus_tag="PLY93_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70511.1"
                     /translation="MKENTNTLATSVKSKRPPWIEKICIPTSAASEVHEEEWQSRLAE
                     KMRTMRGGDLRQPPVSVKGEAITNHFAFHISPLGHGDFDNVPVEPSVKKNQSYSTANE
                     ADISTQESFVDADIKDSTTEEVEFAQPRTKAAAQSGDNYVLHS"
     gene            complement(1096..>1615)
                     /locus_tag="PLY93_13370"
     CDS             complement(1096..>1615)
                     /locus_tag="PLY93_13370"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRP70512.1"
                     /translation="DVSYAAAKELGFVEKGVAKVHLELVQAGEDNFVSKVDSPSKQTK
                     RSTKKIVPEDEIIEPVDFDDSIDAAADDDDEEIVTHKTTKKSNGKLVFLDGKKPKGYT
                     IQVGAFKKKINAERHRDELLESYHQKSFIATNGKWHFVCMGDFKTREQAKAFLKKLSD
                     DGVDVMYRGKVS"
     assembly_gap    1616..1666
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1667..>1969)
                     /locus_tag="PLY93_13375"
     CDS             complement(<1667..>1969)
                     /locus_tag="PLY93_13375"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRP70513.1"
                     /translation="KAFIVALFVVFLVACRAPQARTSPNDPAQQAQNQDGQDPVLQDG
                     APQEDRASTLVSEGYASWYGTELQGRATASGESFDMNKMTAAHRDFPMNSLVLVKN"
BASE COUNT          402 a          451 c          423 g          598 t
ORIGIN      
        1 tcgagagttt cttcgagctt ttcgataact tgttgcgctt cggcgggatt agtgatttcc
       61 aaagcgcgtt tctgtgtctt gagcatcggg aaaagtaatt cgcgctgacg gctcacttgt
      121 aagaccggcg ttatagatgc gcgctccacg agtatcggct ttttaagttc gggggtgatt
      181 tttgcttctc taggtttagg tatattttct actgctatcg tacgcggttt ttcttcagtg
      241 gctggaagta tggtttcgtc ttcgtattct tcggaggtat ctattttgaa ctccaaattt
      301 tcagcaaagg atggagttga gggtacagca tctattgtat tgttactctc tgctaccgta
      361 ctttcaccaa aaataatttc agtaatatcg tagctgactt ttgattcact ggagctatac
      421 gcgccaccac tcggctctat atcgccatcg gtcaactgtt gaattgcttc ctcgggtgca
      481 gaccatgcgc tcggcgatgc cttggcgccg tcctcaaaaa gttgctcgat atcaaagtcg
      541 tcgccaccgc catagtcaac ggtggaatgc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnncgag tgtagaacat agttatcccc actttgtgcc gctgctttcg
      661 tgcggggttg tgcaaattcc acttcttctg tggtcgagtc ttttatatca gcatctacaa
      721 aactctcttg ggtagaaata tctgcttcgt ttgctgtgga atacgactga ttttttttaa
      781 cgcttggctc aaccggcaca ttgtcaaaat ccccgtgtcc caaagggctg atgtggaatg
      841 cgaaatgatt tgttatcgct tcgcctttaa ccgataccgg aggctggcga agatcaccac
      901 cgcgcatagt gcgcattttt tccgccaagc ggctttgcca ttcttcttcg tgtacctcag
      961 aggcagcgct tgtggggata caaatttttt caatccaagg tggccgcttg gacttaacgc
     1021 ttgtggcgag agtattcgta ttttctttca agacacccta ctttctcgcc acgatttaca
     1081 aagactctct ttggtttagc ttactttacc acggtacatg acatccacac cgtcgtcgct
     1141 caattttttc aagaatgctt ttgcttgttc gcgcgttttg aaatcaccca tgcaaacaaa
     1201 atgccatttt ccgttggtgg caataaaact tttttggtgg taactttcta agagttcatc
     1261 gcggtggcgt tccgcattta tttttttctt aaacgcaccc acctggatag tgtatccttt
     1321 gggctttttg ccatcgagaa aaaccaactt gccatttgat ttttttgtag tcttatgcgt
     1381 cacaatttct tcatcgtcat catcggcggc ggcgtcgatg gagtcgtcaa agtcaaccgg
     1441 ttcgatgatt tcatcttcgg ggacgatttt ttttgtactg cgttttgttt gtttggaagg
     1501 ggaatctacc tttgagacaa aattatcttc accggcttgc accaattcta aatgcacttt
     1561 cgccactcct ttttcgacaa agcccaattc ttttgccgca gcatagctca catcgnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat ttttaacaag
     1681 gactaacgag ttcattggga aatcgcggtg cgctgccgtc attttattca tatcgaacga
     1741 ctcgcccgat gcggtggccc gcccttgcaa ctctgttcca taccacgacg catagccttc
     1801 gctcaccagc gtggatgcgc gatcttcttg tggtgcgccg tcttgtaaca ctgggtcttg
     1861 accgtcttga ttctgcgcct gttgtgcagg atcattgggg ctcgtgcgcg cctgtggagc
     1921 acggcatgca accagaaaaa caacaaataa tgcgacaata aaagctttc
//
LOCUS       DAOMPD010000502         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22388_length_1966_cov_3.368394, whole genome shotgun sequence.
ACCESSION   DAOMPD010000502 DAOMPD010000000
VERSION     DAOMPD010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22388_length_1966_cov_3.368394"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..1870)
                     /locus_tag="PLY93_13380"
     CDS             complement(419..1870)
                     /locus_tag="PLY93_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRP70514.1"
                     /translation="MKKLIGFFFLAILLQSLVFGAQDIHEAEKINNDTSGHVLWASEY
                     VLDLYLPQIHTESERSYLGGVVQNLIADELALQRRVITNGKITASVIEYSVRKTDEPL
                     VPNEREELLNRFPKPKVLPLKVVRLASTPSVFSCKELPPNRYSLLTQVSGDVRSVRID
                     LLLCHGTNALLQKTSGAKEQDLVLSIHRLMNPVRAKITGNEYASLAITTTPSNASVYI
                     DNQFVGKTPLSYSYLIPDKYTLVIKRDGYEPFTTNFVAARGDVIDKSYSLKPAQAMGL
                     IQIDSEPSGAKVYLDADYKGVTPKVLDKLQFGTYRLHVLKEDAGEIYQNIILSEKNSV
                     VKVSASLTEFLAKTPAGFWGISYKGWYWVSLTAAALSFGTAIAFYVWRDDAKEDVYAR
                     LSGKSLSMYTADDYAFRDERNAAYKTREDYATGFMVGAGVFAVAAIYFYVQSLLSADE
                     GIVMNKTPSKAKGFDFAIGASVEHTDLTLRFRF"
BASE COUNT          588 a          443 c          387 g          548 t
ORIGIN      
        1 aattcaaaaa ttcaccgagc accaggcgct gtttttcttt catcgagaat aaatgcgcca
       61 ttttgagagt gattttttcg gcgatatttt cttgatctat gtcattgtct ttagccatat
      121 aactcgttga attctcacca tatttcatta accaagaaca taacacattt ttctaaaatg
      181 tcaattcgtg gttattctaa caaaagtacg tagttatact tattattttt gagagcaatc
      241 gtttacaaaa aaataaaaaa attttgtaaa agacgagagg ctctggtacg caagaaatcc
      301 gaagcaaagc cagccggtta ggcaaaatca cagcctcttg cgggctaccc tttccaaagc
      361 ggtgcatagt taccccccaa tgggacataa tacggcaaaa aatggccaac gagtagattt
      421 aaaaacgaaa ccgcagcgtt aaatcggtgt gttcgacaga ggcaccaata gcaaaatcaa
      481 atcctttcgc tttcgatgga gttttattca tcacaatgcc ttcgtcggcg gacagaagtg
      541 attgtacgta aaaataaata gcagcaactg caaagacccc cgcaccgacc ataaaccctg
      601 tcgcatagtc ctcacgcgtt ttgtatgcag cgttacgctc gtcgcggaaa gcgtaatcgt
      661 cggcggtata catactgagt gacttcccgc ttaaccgagc gtaaacatct tcttttgcat
      721 catcgcgcca tacataaaaa gcgatagcgg tgccaaaaga caacgctgca gcggtgagcg
      781 atacccaata ccatcccttg tatgaaatcc cccaaaagcc agcaggagtt tttgccagaa
      841 attcggtaag tgatgctgaa actttcacga cagaattctt ttcgctcaaa atgatatttt
      901 gatatatttc acccgcatct tctttcaaca catgtagccg gtaagtaccg aactgcagtt
      961 tatctaaaac ttttggcgtt actcctttat agtcggcatc gagataaacc tttgcgcccg
     1021 atggctccga atcgatttga atcaatccca ttgcttgagc gggttttaat gaatagcttt
     1081 tgtcaattac atcgccacga gctgcaacaa aatttgttgt aaacggctcg tacccatcgc
     1141 gctttattac aagcgtgtat ttatcgggta taaggtatga ataactcaac ggggttttac
     1201 caacaaattg attatcaata tacaccgacg cattcgatgg cgttgtcgtt atcgcaagtg
     1261 atgcatattc attacctgta atttttgcac ggacagggtt catcagccta tgaatactca
     1321 gcacaaggtc ttgctctttt gccccagaag ttttttgcaa cagcgcgttt gtgccatgac
     1381 aaagcaataa atcaatccga acactgcgta catcacccga cacttgtgtc aataatgaat
     1441 accgattagg aggcaattct ttacatgaaa aaaccgaagg cgtactggcg agccgaacta
     1501 ctttcagcgg caagacttta ggtttgggaa atcgattgag taactcctca cgttcgttgg
     1561 gaacaagcgg ctcatctgtt ttgcgcaccg agtattcaat gacagatgcg gtaatttttc
     1621 cattagtaat gacgcgtcgc tgcaatgcga gttcatcggc aattaaattt tgcacaacac
     1681 caccaaggta gctgcgctca ctttcagtat gtatttgcgg taaatataaa tctaatacat
     1741 attcactcgc ccaaagtaca tgccccgatg tatcgttgtt tattttctca gcttcatgaa
     1801 tatcttgcgc accaaaaaca agagattgga ggagtatcgc taaaaagaag aatccaatta
     1861 attttttcat aacccaaaga ttatgctgcc gaggctaaat gcttgatacg ttggatcatc
     1921 gaagcaaagc cgttcgcccg cgacatcgat aaatgtttgg caaagc
//
LOCUS       DAOMPD010000503         1965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22416_length_1965_cov_2.179058, whole genome shotgun sequence.
ACCESSION   DAOMPD010000503 DAOMPD010000000
VERSION     DAOMPD010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22416_length_1965_cov_2.179058"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLY93_13385"
     CDS             <1..876
                     /locus_tag="PLY93_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70515.1"
                     /translation="RGLIAFLMSLVLVASLFFIGVAVSIKRMVINPTFYADQIDQLDF
                     SRTADELERAKVKPGASSQKGQLHSFSYMLPLVLKNFEAPLKKQTRALLILSADYFKG
                     KSKTLELKYDLSALLQDSAFKNKLKEFLQKKADFLPDFLNNIAVDEAAELLPREFDLL
                     KYAKFSRTNVEEAQAMTISLYKKFENYYMAALVVSSVLLIVLFFAIKSLRNYYFTLAV
                     IFLLSGILLFTPYTYFDSFFKNLGGDLFTFLKEIGYVKLFAAAVKSAFLFSPLVCLGI
                     AGVSLFLGLTKKEST"
     gene            1000..>1965
                     /locus_tag="PLY93_13390"
     CDS             1000..>1965
                     /locus_tag="PLY93_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRP70516.1"
                     /translation="MQSLEAYLSIGILILLLGISLVSTFYLRFVPLRYIFFGFRIALT
                     GLGREDEGTTLPRTQRAGEINNLRAMFLSGSIQANAAVLLGIPVVVLWGGVSSLIWVI
                     LFGILLASLRFASGTLAVRYRQKTESGWLGGAQEFLALTFWGRSGMLVYVILAIAITL
                     VWGAAVQADLWQNFYKIPAKAKISIKIGEHFFASPSIPLIMALIGLFLSLGSMRRVAR
                     VAAFISPIVSGLLILLSLFIIAQNSAKASQFLSNAWAHAFHFKSIAAGVLTALVAFKT
                     IAYTGIVAPEEATGISSAVTAASINDYAVKQGLVSMSAALFYTTIV"
BASE COUNT          465 a          418 c          466 g          616 t
ORIGIN      
        1 cgagggttga ttgctttctt aatgagttta gtgttggtgg cgagcctttt tttcatcggc
       61 gttgctgtct ctataaagcg catggtcata aaccccactt tttatgcaga ccaaattgat
      121 cagttggatt tctctcgtac cgcggatgaa ttggaacggg caaaagtaaa acctggagca
      181 tcgtcacaaa aaggacaatt gcattcattt tcgtatatgt tgccgttggt actcaaaaat
      241 tttgaagcgc cattaaaaaa acaaacgcgc gcattgctca ttctctcggc ggattatttt
      301 aagggtaaaa gtaaaacgct tgaattaaag tatgatttaa gcgcgctcct acaagacagt
      361 gcgtttaaaa ataagctgaa agagtttttg caaaaaaaag cggattttct tcccgatttt
      421 ttaaataaca ttgccgttga tgaagctgct gaacttttgc cgcgtgagtt tgatttattg
      481 aaatatgcaa aattttcaag aacaaatgtc gaggaagcac aagcgatgac cataagccta
      541 tacaaaaaat ttgaaaatta ctatatggct gctcttgtcg tctcttctgt gttgttgatt
      601 gtactatttt ttgctataaa aagcttacgt aattactact ttactttagc ggtaattttt
      661 cttttatcgg gtattttgct ttttacaccg tatacgtact ttgatagttt ttttaaaaat
      721 ttaggcggcg atctttttac ttttttgaaa gagattgggt atgtaaaact ttttgccgca
      781 gcggtgaagt cggcgttttt attctctcct ttagtgtgtt tgggaatagc tggcgtatcg
      841 ctatttttag gacttaccaa aaaagaatcg acgtgaggag cccgtgcgcc aacctagtga
      901 ggagtctgca cgagttgacg cacaggctct tgaagcaact tccatcaagc tgccccattt
      961 catgcttgcc gtacactctt tcagctcaag tatgaaccga tgcagagcct cgaagcctat
     1021 ctttccatcg gaattttaat cctccttttg gggatttccc tcgtttcaac tttttatttg
     1081 cgcttcgtac cgctgcgcta cattttcttt ggcttccgca tcgctctaac ggggttggga
     1141 cgtgaagacg aaggcacgac actgccgcgt acacagcgtg cgggtgaaat caataattta
     1201 cgcgcgatgt ttttgtcggg cagtatccaa gcgaacgctg ccgtgctttt gggcataccg
     1261 gtggtggtgc tttggggcgg agtttcttcg ttaatttggg tgattctttt tggaatttta
     1321 ctggcgtcgc tgcgttttgc ctcgggcact ctcgcggtgc gataccgtca aaaaaccgag
     1381 tcgggttggt tgggcggagc gcaagaattt ttggcgttaa ccttttgggg gcgttcgggg
     1441 atgctggtgt acgtcatttt ggcgattgcg attacactcg tgtggggcgc agcggtgcag
     1501 gcagacctct ggcaaaattt ttacaaaatt cccgctaaag caaaaatctc aataaaaatt
     1561 ggggagcatt tttttgcttc gccgtcgata ccgctcatca tggcgctcat cggccttttt
     1621 ttaagtttag ggagtatgcg ccgcgtggcg cgtgtggcag cgtttatttc gccgattgta
     1681 tccggacttt taatcctact ttcgcttttt atcatcgcgc aaaactcggc aaaggcatcg
     1741 cagtttttat ccaacgcttg ggcgcatgca tttcatttta aaagcattgc cgcaggtgtg
     1801 cttaccgcgc tcgtagcgtt taagaccatc gcatacacag gtatcgtcgc ccccgaagaa
     1861 gccactggaa tttccagtgc ggtgaccgcg gcgtcgatca acgactacgc cgtaaaacaa
     1921 gggctcgtct cgatgtcagc ggcgctcttt tataccacga ttgta
//
LOCUS       DAOMPD010000504         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22494_length_1959_cov_2.556723, whole genome shotgun sequence.
ACCESSION   DAOMPD010000504 DAOMPD010000000
VERSION     DAOMPD010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22494_length_1959_cov_2.556723"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="PLY93_13395"
     CDS             <1..1444
                     /locus_tag="PLY93_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70517.1"
                     /translation="RIFDSSGAWQTTTTNSFGEFIINGTFAHPPDRIGSRAFASDVVT
                     GLSISTADLRVGMLVKGTSLPVGTKIIQILSANSVRLSDQSTATVADNLQFMRLNDVE
                     ITKSGYTGATEVSKQIFQFGHDGSATCPATPYNLDTNINACGVTDVGPVGAKNCTGNL
                     VLYPIGIFAPMGNFKHQIKQTYEKFLTEKAGLTISARTGSLVLTSGAQRNLNFDGFYL
                     HFDDTPKALPNVPEGKWSNHVPVNAMSTKCTPTVTTNCSPRANGVFAEGIGDDTRATP
                     WGLKTYVYYNFYAAAAGSYTIQTTGGTDTQITLYAQTGANMGSDDDSGSGSNAKMTLN
                     LLRGWYYIKVFPKNNNTFGFFDVEVTGPTQDESNYSAMLTPTTPSSVDYQTVNGTASY
                     QTNCAPGNGNLVLSWYNHTDHLLYIAAPGENGGCNAKAWLEKHDAIGGIIRGRFEGVL
                     RPIAPGGSNQTIPPNTGPGIGGFFNIVRME"
     gene            1446..>1959
                     /locus_tag="PLY93_13400"
     CDS             1446..>1959
                     /locus_tag="PLY93_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70518.1"
                     /translation="MRSFLVGLFSVTALFAVSEGELTFSVAGDNYVSHGAQGVLQTLK
                     NQKRIVVAVKDRDAKFLFALTVDVPQESETQTLHLNTHDSVMSVVFRSTKGSMAIMPV
                     VQFAKEDPNAIEIERIEKETNEYESESPADAKLRKRRHNDPHPPRKKLHVEYRRVKPR
                     WHSMTKEERLR"
BASE COUNT          536 a          471 c          477 g          475 t
ORIGIN      
        1 gcgtattttt gatagttcgg gggcctggca aacgacaacc acgaattcat ttggcgaatt
       61 tattatcaac ggtactttcg cacaccctcc cgacagaata ggctcgcggg cgtttgcgag
      121 tgatgtggta accggtttat cgatttccac agcagatttg cgcgtgggta tgttagtcaa
      181 agggacgagt ttgcctgtag gcacaaaaat tattcaaatt ctgtcggcga acagtgtccg
      241 cttgagcgat caatccaccg caacggtcgc ggacaatttg caatttatga ggctcaacga
      301 tgtagaaatt actaaatctg gttatacagg agcaaccgag gtctcaaaac aaatttttca
      361 atttgggcac gatggttctg caacctgccc tgcgactccc tataatttgg acacgaatat
      421 caacgcgtgc ggtgtcaccg acgtaggccc ggtgggggca aaaaattgca cggggaattt
      481 agttctctat cccatcggta tttttgctcc gatgggtaat ttcaagcatc aaattaaaca
      541 gacttatgag aagtttttga ctgaaaaagc aggcttaacc atttctgcgc gtacgggtag
      601 tcttgtgctg actagcggtg cacaacgaaa cttaaatttt gatggtttct atttacactt
      661 tgacgatacc cccaaagcgt tgccgaatgt gccagaggga aagtggtcaa accatgtacc
      721 ggtgaatgca atgagtacaa aatgcacgcc aacagtaacg accaactgtt cgccccgcgc
      781 caacggagtt tttgccgaag gcatcggcga cgacacccgc gccacgccgt gggggcttaa
      841 aacctacgtg tactataatt tttatgcagc cgcagcgggc tcctatacta tacagacaac
      901 aggcggcacc gacacccaaa tcacgctcta tgcgcaaacc ggcgctaaca tgggttctga
      961 cgacgattct ggttcaggct ccaatgcaaa aatgacgtta aatcttctaa ggggttggta
     1021 ttatatcaag gtattcccca aaaacaacaa cacatttggt ttttttgatg ttgaagtaac
     1081 cggtccaact caagacgaaa gtaactattc agcgatgttg actcccacaa ctcctagttc
     1141 agtggactac caaaccgtga atggtaccgc gtcttatcaa actaattgcg cgccaggtaa
     1201 cggcaatttg gtgttatcgt ggtataatca taccgaccat ttgctctata tcgccgctcc
     1261 gggagaaaat ggaggatgta acgctaaagc atggcttgaa aaacatgacg ccatcggtgg
     1321 tatcatccgt ggtcgttttg aaggagtgtt gcgccccatt gctccagggg gatcgaacca
     1381 aacaatcccc cccaacaccg gccctggcat tggtggtttt tttaatatcg ttcgcatgga
     1441 gtaacgtgcg ttccttcctt gtcgggttgt tttcggttac agcgcttttt gcggtaagcg
     1501 aaggggaact cacgttttct gtggctgggg ataactatgt ctcgcacgga gcgcaagggg
     1561 ttttgcaaac gttaaaaaat caaaaacgca tcgtcgttgc cgtaaaagat cgcgatgcaa
     1621 agtttctttt tgcgctcacc gtcgacgtgc ctcaagagag cgaaacacag acattgcatt
     1681 tgaacaccca cgattcggta atgagcgtgg tgttccgttc gacaaaaggc tcaatggcca
     1741 tcatgcccgt tgtgcagttc gccaaagaag accccaacgc aatagaaatt gaacggattg
     1801 aaaaagaaac aaacgagtat gaaagcgaga gtcctgcgga tgcaaaactg cgtaaacgcc
     1861 gccacaacga cccccacccg cccaggaaaa aattgcacgt cgaataccgc cgcgtgaagc
     1921 cgcgttggca ttcaatgacg aaagaagaac ggctgcgct
//
LOCUS       DAOMPD010000505         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22536_length_1957_cov_1.827024, whole genome shotgun sequence.
ACCESSION   DAOMPD010000505 DAOMPD010000000
VERSION     DAOMPD010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22536_length_1957_cov_1.827024"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLY93_13405"
     CDS             complement(<1..796)
                     /locus_tag="PLY93_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRP70519.1"
                     /translation="MDKERKIRLTVGIMTLVSLATFVALLAILGRWQMAGDGLKINLR
                     FRFLNNLSTGAPVRISGGLPVGFVESIYQKDLKTYVQISLKKELLNKIPKRAETVFAI
                     YTTGLMGQKYINITVPDGKENEVYFQSGDEWVGIDPPSVDQMMMAFSSWFDGKNGGQV
                     LAEIMKETETFLSNLNSIVNENRGDIRTTIKQARASFTSLSAQLDTLMQKLNILSKNF
                     TDISNQNKQDIQIMLENMSKISRDLNLITQRINSGRGSVGKLIQDEE"
     gene            complement(806..1567)
                     /locus_tag="PLY93_13410"
     CDS             complement(806..1567)
                     /locus_tag="PLY93_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP70520.1"
                     /translation="MAGETHIHLDNVSKSFGTKHILRGINLEIKRGEIIYIIGKSGSG
                     KSVTLKNITGLMRPEPGRVLIDGHDIHAVDNTTLNEIRKKMGVLFQMAALFDSMSVFE
                     NVAFAPRRFTKLTMEEISALVKEKLTLVGLKDVERLNPSALSGGMQKRVGLARAIAMS
                     PEIVLYDEPTTGVDPILAAAIDDLIRSLNTKTGVTSIVISHDMASVLRTADRVAMLYD
                     GVFRRVGTTEEFKNSTDPVVRQFVEGNAEGPIPIL"
     gene            complement(1600..>1957)
                     /locus_tag="PLY93_13415"
     CDS             complement(1600..>1957)
                     /locus_tag="PLY93_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRP70521.1"
                     /translation="SVPRFLAALFSIPMITVMAIVVGVFGGALISNISLDIPYNLYFD
                     SARTLVRSTDLISGVGKTFFFGAEIALISCFSGFRARGGAEGVGQATIQAVVFSFMMI
                     IVSDYFITYIFQLFGL"
BASE COUNT          497 a          458 c          495 g          507 t
ORIGIN      
        1 gctcttcgtc ttggatgagt ttacccaccg agccgcgccc ggagttgatg cgctgggtga
       61 tgagatttaa gtcgcgagaa attttagaca tattttcaag cattatttgg atgtcttgct
      121 tgttttgatt tgagatgtct gtaaaatttt tggaaaggat gtttagtttt tgcatgagag
      181 tatcgagctg cgccgaaagc gaagtgaaag acgcgcgcgc ttgtttaatc gtggtgcgga
      241 tgtcgccacg gttttcgttc acgatactgt tgaggtttga caaaaacgtt tcggtctctt
      301 tcatgatttc tgccaagact tgaccgccgt ttttaccgtc gaaccaacta gagaacgcca
      361 tcatcatttg gtcgaccgac ggcgggtcga tacccaccca ttcgtcgccg ctttgaaaat
      421 agacttcgtt ttctttgccg tcgggcacgg tgatgttgat atacttttgc cccataaggc
      481 cagtggtgta gatagcgaac acggtttcag cacgcttggg gattttattg agaagctctt
      541 ttttgagcga aatttgtacg tacgttttca aatctttttg atagatgctc tcaacaaacc
      601 ccacgggaag cccccctgaa attcggaccg gcgcgccggt ggaaagatta ttcaaaaagc
      661 gaaagcgtaa gttaattttt aagccatcgc ctgccatttg ccaacgcccc aaaatggcga
      721 gcagcgccac gaaggtggcg agcgagacga gcgtcattat gcccactgtt aaacggattt
      781 tgcgttcttt gtccataaat tttccttaca aaatagggat tggtccctcg gcattgccct
      841 cgacaaattg tcgcactaca gggtcggttg aatttttgaa ttcttcggta gtgccaaccc
      901 tgcgaaatac tccgtcgtaa agcatcgcca cacgatcggc ggtgcgtaac accgacgcca
      961 tgtcgtgtga aatcacgatg gaggtaacgc cggttttggt gttgagcgag cggatgagat
     1021 cgtcgatcgc agcagctaag atagggtcaa caccggtggt gggctcgtcg tagagcacaa
     1081 tttcaggact catggcaatc gctcgtgcaa gccccacacg cttttgcatg cctccggaga
     1141 gcgccgatgg atttaagcgc tccacgtctt taagccctac gagagtgagt ttttctttga
     1201 ccagagcgct gatttcctcc attgtcaatt tagtaaaacg acgtggcgca aaagcgacgt
     1261 tctcgaaaac agacatcgag tcgaagagcg ccgccatttg gaagagcact cccatttttt
     1321 tgcgaatttc attgagcgtt gtattatcta cagcgtggat gtcgtggcca tcgatgagga
     1381 cgcgtcctgg ctcggggcgc atgaggccgg tgatgttctt gagcgtcaca gacttccccg
     1441 acccgctttt accgatgatg taaataattt caccacgttt gatttctaag ttaatcccgc
     1501 gcaaaatatg ttttgtgcca aaactttttg aaacgttgtc gaggtggatg tgtgtctcgc
     1561 cggccataag caaagttcag tgtaaatccg ccttggtatt cataacccaa ataactgaaa
     1621 aatatacgtg ataaaataat cggagacgat aatcatcata aacgaaaaga ctaccgcttg
     1681 gatggtcgct tgccccacgc cttcggcgcc gccccgagcc cgaaaaccag agaaacacga
     1741 aatgagggcg atttctgcac cgaagaaaaa agttttaccc acccccgaaa tgaggtcggt
     1801 ggaacgtaca agagtgcgcg ctgaatcgaa ataaaggttg tatggaatat ccaagctaat
     1861 gtttgagatg agagcgccgc cgaaaacgcc caccacaatc gccatgacgg taatcatggg
     1921 gatggaaaaa agcgcggcta aaaatcgcgg cactgat
//
LOCUS       DAOMPD010000506         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22559_length_1955_cov_3.005789, whole genome shotgun sequence.
ACCESSION   DAOMPD010000506 DAOMPD010000000
VERSION     DAOMPD010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22559_length_1955_cov_3.005789"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLY93_13420"
     CDS             <1..774
                     /locus_tag="PLY93_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HRP70522.1"
                     /translation="FTKNDAAHTPVYVTYRPDMSLNDVIHAINRSDAGIVAYLNHNNN
                     LVLKGREASDNYLTNFMIRHMEDSGEFLTGFSGLLQNSGAAGAFDYRRTDEINKLQST
                     LDRITLTSVVHPAGAMHLSNEVEGNTALIAAALGKDVGGTGDANMAYGAKDGGNATRI
                     AQAMRHKPTMVGEYRNSDEFYNALIAKAGIESKTAKEQVENQKLIMTNLENMRQSVMG
                     VNLDEEMAAMVQFQHGYNAAAKIMQTMNEMLDRLINHLG"
     gene            complement(787..1182)
                     /locus_tag="PLY93_13425"
     CDS             complement(787..1182)
                     /locus_tag="PLY93_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP70523.1"
                     /translation="MAFDILLQADAMQHRYMRRDDFVYRVGDECDGYVFVVIEGTVGA
                     VDTFGNFKEGYEVTRGGFFGDIEVMTGNPLRLQGFRVKTISATLAMMDKRAVQLVGGI
                     HSQFFLNLLQSAVDNLRYAEEELLKKPRT"
     gene            complement(1172..1540)
                     /locus_tag="PLY93_13430"
     CDS             complement(1172..1540)
                     /locus_tag="PLY93_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP70524.1"
                     /translation="MSESQKIQPLHLEKGAVVFAEGEVSNGCMYFIFSGEVLITKSGF
                     GVIRSLSQGHFFGEMALVRAIPRSATAIVASATAKLGCIDLKTFAWLAKNKPIFLSNL
                     ISVVAKRAARAMQKVVDRGL"
     gene            complement(1549..>1955)
                     /locus_tag="PLY93_13435"
     CDS             complement(1549..>1955)
                     /locus_tag="PLY93_13435"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP70525.1"
                     /translation="GDGILATFGCPIPKEHDAANAVVAACAIHKHMASINRLREIAGK
                     VPYGFGIGIATGKIFAGNIGSERMIKYAVMGDAVNTAARIQDLTKGLPYAIIIDEPTR
                     AAGNPTCSLEELTTTQLRGKNEPVQLFGVLKV"
BASE COUNT          533 a          503 c          453 g          466 t
ORIGIN      
        1 tttacaaaga acgatgcggc gcacacccct gtctacgtga cgtatcgccc cgacatgtcg
       61 ctcaacgatg tcatccatgc catcaatcgc tctgacgctg ggattgtggc gtatcttaac
      121 cacaacaata atttagtgct taaaggccgc gaagcgtcgg ataactatct caccaatttt
      181 atgatccgcc atatggaaga ctcgggtgag tttttaaccg gcttctcggg ccttttgcaa
      241 aactcgggcg cagccggcgc gtttgattac cgacgcaccg atgaaatcaa caaactacaa
      301 tcgacattgg acagaatcac tctcacgtcg gtggttcatc ccgcgggagc aatgcacctc
      361 tcgaatgaag tcgaaggtaa caccgcgctc atcgccgctg cgctgggtaa agacgtgggc
      421 ggtacgggcg atgccaacat ggcttatggc gcaaaagacg gcggtaatgc aacgcgcatt
      481 gcgcaagcga tgcggcacaa accaacgatg gtgggtgaat acagaaacag cgacgaattt
      541 tataacgcgc tcatcgcaaa agcgggcatc gagtcaaaga ccgctaaaga acaagtcgag
      601 aatcaaaaac tgattatgac aaacttagaa aatatgcggc agtcagtgat gggcgtcaac
      661 ctcgacgaag agatggcggc gatggtacag ttccagcacg gctacaatgc agccgcaaaa
      721 attatgcaaa cgatgaacga gatgctcgat cggttgatta accatttggg ataaaaggtt
      781 gtttgattaa gtgcgcggtt tttttagtaa ttcctcttcg gcgtagcgca aattatccac
      841 agcgctttgt agaaggttca agaaaaactg ggagtgaatc ccgccaacga gctgcaccgc
      901 gcgtttatcc atcatggcga gtgttgccga tattgtcttc actcgaaagc cttgtaaacg
      961 taagggattg cctgtcatca cttcgatatc gccaaaaaaa ccaccccgcg tgacttcata
     1021 accttcttta aaattgccga aggtatcgac agcccccaca gttccctcaa taacaacaaa
     1081 cacataaccg tcgcactcgt cgcccacacg gtagacaaaa tcgtcgcggc gcatataacg
     1141 gtgctgcatg gcatcggctt ggagcaatat atcaaaggcc acgatcgacc accttttgca
     1201 tagcgcgtgc cgctctttta gcgactacac tgatgagatt agataagaaa ataggtttat
     1261 ttttagccaa ccatgcaaat gtttttaagt cgatacaccc taactttgcc gttgccgagg
     1321 cgacgattgc agttgccgaa cgtggtatcg cccgtacaag cgccatttcc ccaaagaaat
     1381 ggccttgtga aagagaacgg ataacgccga atcccgattt tgtaatgagc acttcgcctg
     1441 aaaaaataaa atacatgcag ccattcgaga cttccccttc ggcgaacacg acggcgcctt
     1501 tttctaaatg aaggggttga attttctggc tctcactcat aattttttct ataccttcaa
     1561 aacgccgaaa agttgaacgg gttcgttttt acctctcagt tgagttgtcg tcaactcctc
     1621 caacgaacaa gttggattcc ccgctgcacg cgttggctcg tcgatgatga ttgcgtatgg
     1681 taaacctttt gttaagtctt gaatacgcgc tgcggtgttg acggcatccc ccatcaccgc
     1741 gtatttaatc atacgttctg aaccgatgtt gccggcaaaa attttacccg tcgcgatacc
     1801 gataccaaat ccataaggaa ctttacctgc gatttcacgc aaccggttga tcgacgccat
     1861 gtgtttgtgt atcgcgcacg cagcaaccac tgcatttgcg gcatcgtgct ctttgggtat
     1921 gggacaacca aacgtcgcca aaataccgtc accca
//
LOCUS       DAOMPD010000507         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22671_length_1946_cov_2.263882, whole genome shotgun sequence.
ACCESSION   DAOMPD010000507 DAOMPD010000000
VERSION     DAOMPD010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22671_length_1946_cov_2.263882"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLY93_13440"
     CDS             complement(<1..799)
                     /locus_tag="PLY93_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRP70526.1"
                     /translation="MRMLVVGSVAFDDIQTPFGTKKNVLGGAASHFSIAASPLVAPKI
                     IAVVGNDFPQKYFTYFDKFDIDTSGIERSSGKTFHWSGFYQHDMAVAHTRSTELGAFA
                     MFEPKLTEEDKLRQVIFLANIDPVIQYKVLKQMKKTQLVGFDSMNFWIESKRKDVWKM
                     VKEADIVFLNDAEVRMLAEEASLIKAAKIIQKKGPKAVIVKKGEHGVTAVTKNEIFVS
                     PAYPTEKVIDPTGAGDSFAGGCMSYLVANTQPGKPLTSDILRKAVSWG"
     gene            complement(842..>1190)
                     /locus_tag="PLY93_13445"
     CDS             complement(842..>1190)
                     /locus_tag="PLY93_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRP70527.1"
                     /translation="LDEAQGVLCHHLGYFVMTDGFSVKYHKATPLYKDLRVHAQLTSA
                     RSKRLYTKAAIYLGDELLVESKIKWYIIPVRLIERRFNANGNSNSKIPLKDFLEPNRA
                     RAKEIRRRLRTMK"
     assembly_gap    1191..1200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1201..1395)
                     /locus_tag="PLY93_13450"
     CDS             complement(<1201..1395)
                     /locus_tag="PLY93_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRP70528.1"
                     /translation="MTQPTGQTSVVGQQLHHDNCFACGAINHYSFHIPSTFDEKTGEV
                     DFIYQLRQEYEGAPGHAHGGA"
     gene            complement(1453..1656)
                     /gene="rpmF"
                     /locus_tag="PLY93_13455"
     CDS             complement(1453..1656)
                     /gene="rpmF"
                     /locus_tag="PLY93_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRP70529.1"
                     /translation="MAVPKRRTSHTRRRNRRAHNAIGKPNLRACSNCGAYGIPHRICT
                     ACGYYGGKQVLIPRVKKAKADKE"
     gene            complement(1675..>1946)
                     /gene="hisG"
                     /locus_tag="PLY93_13460"
     CDS             complement(1675..>1946)
                     /gene="hisG"
                     /locus_tag="PLY93_13460"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRP70530.1"
                     /translation="TKYPRLATEYFFRRGFNIRTIELYGSIEIAPLTGLSDVIVDLVS
                     TGATLKANNLVEEEVILESSARLIMNPSSFVLRRELLMQFVDTLF"
BASE COUNT          490 a          463 c          458 g          525 t
ORIGIN      
        1 aaccccatga aaccgctttg cgcaaaatat cgctcgtcaa aggtttgccc ggttgggtgt
       61 tcgcgacaag gtagctcata cagccgcctg caaaagagtc gcccgctcct gtgggatcga
      121 tcactttttc ggtggggtat gcaggtgaga caaaaatttc atttttggta acagcggtca
      181 cgccgtgctc gccttttttg acaatcactg ctttagggcc tttcttttga ataattttgg
      241 ccgctttaat gagcgacgcc tcttcggcga gcatacgaac ttcggcgtcg ttcaagaaaa
      301 cgatgtcggc ttctttcacc attttccaaa cgtctttgcg cttcgattcg atccaaaaat
      361 tcatcgagtc aaagcccaca agctgcgtct ttttcatttg cttcaaaact ttatattgaa
      421 tcacggggtc gatattcgcc aagaaaatca cttggcgcaa cttatcttct tcggtgagct
      481 tgggctcaaa catagcaaat gcgcccaact cggtcgagcg ggtgtgtgca accgccatat
      541 cgtgttgata aaaccccgac caatggaatg ttttgccgct actgcgctcg atgcctgagg
      601 tgtcgatatc gaatttatcg aagtatgtaa aatatttttg agggaaatca ttccccacaa
      661 ccgcgataat tttaggagca accaagggag aagcggcaat ggaaaaatga ctcgcagcgc
      721 cgcccaagac gtttttcttt gtaccaaacg gcgtttggat atcgtcaaaa gcaaccgatc
      781 ctacaacaag catacgcata gtttatctcc tttaatcgct aaacccattc tcaaatagat
      841 attatttcat tgtacgcaat ctgcggcgga tttctttcgc gcgcgcgcga tttggttcca
      901 agaaatcttt gagtggaatt ttagaattac tgttcccatt agcgttgaag cgccgctcga
      961 tgaggcgtac ggggatgatg taccatttaa ttttagattc caccaaaagc tcgtcgccta
     1021 aataaatcgc cgctttagta taaagccgtt tggagcgcgc cgaggtgagt tgagcatgta
     1081 cacgtaaatc tttgtagagc ggcgtcgctt tatggtattt aaccgaaaaa ccgtcggtca
     1141 tcacaaaata acctaagtga tggcaaagca caccctgcgc ttcgtcgagg nnnnnnnnnn
     1201 tgcaccgccg tgcgcgtggc cgggagcacc ctcgtactcc tgacggagtt ggtagataaa
     1261 atcgacttcg cctgtttttt cgtcgaacgt cgacggtatg tgaaaagaat aatggttgat
     1321 tgcgccgcaa gcaaaacaat tatcgtgatg gagttgttgc cccaccacac tcgtctgtcc
     1381 cgtaggttgc gtcatgatta acgcgcccac gctatctgag tctgtgtacg gttaacgcac
     1441 aaacccatgc ctttattctt tatcggcttt tgcctttttg acgcgtggaa tcaatacttg
     1501 cttgccgcca taataaccac atgcagtaca aatacggtgt gggataccat acgcgccaca
     1561 gttggaacaa gcgcgtaaat tcggcttacc gattgcgttg tgcgcgcggc ggttgcgacg
     1621 acgtgtgtgt gatgttcttc ttttgggtac agccattgta gtcgtaaaat gaaattaaaa
     1681 caaggtgtca acgaattgca tgaggagttc ccgacgcagt acaaacgacg agggattcat
     1741 gatcaaccgt gcgctgcttt cgagaatcac ttcttcttcg actaaattat ttgctttaag
     1801 cgtcgctccg gtggagacta aatcgacaat cacgtcggat agccccgtca acggcgcaat
     1861 ctcaatagaa ccgtagagtt caattgtgcg gatattgaat cctcgccgaa aaaaatattc
     1921 ggtagccaga cgggggtatt tggtcg
//
LOCUS       DAOMPD010000508         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22673_length_1946_cov_2.061872, whole genome shotgun sequence.
ACCESSION   DAOMPD010000508 DAOMPD010000000
VERSION     DAOMPD010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22673_length_1946_cov_2.061872"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLY93_13465"
     CDS             <1..367
                     /locus_tag="PLY93_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRP70531.1"
                     /translation="GPGVAIRILTAVTKENIATLRDADAILLDELKASGWYEKVWQAG
                     AIFLPVRSVGVMGDNRTYENVIALRLVTSEDGMTADFAHIPYDVLSKIASRIINEVRG
                     VNRVVYDISSKPPATIEWE"
     gene            380..973
                     /locus_tag="PLY93_13470"
     CDS             380..973
                     /locus_tag="PLY93_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRP70532.1"
                     /translation="MRRFIRIFFFFCCTPIYFSLAADSWKNEWRQEESPSKENRDKSK
                     GSDRLVKELDEVWRVVFKNAKGEDVVLDCEVARSEKDKERGLMFRKFLGKNRGMIFVY
                     EEPHEMRFWMQNTVLPLSIAFIHSNLFISSLHDMKPMSLDIVASEVPTLYAIEANQGW
                     FKTNTILPGNRLTIYKKKDEYKIKRTNKYRQALPEVK"
     gene            1064..>1946
                     /locus_tag="PLY93_13475"
     CDS             1064..>1946
                     /locus_tag="PLY93_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70533.1"
                     /translation="MRRILLLVGFSPLLLFLYCKEKTLNKPAVEIQKIFWSSPEEVLK
                     PTIWDTGDTTVTNEDRLEIFQPHIAGIGGGYFGVGSIQNFTLAAWARSEYIWLMDFTR
                     VVVAANKIHTAFLKRLSGPEEHRLMWDKKKSATAVDKIIDKEFKNDPDLAFIKQSYRK
                     ASPYLMITFGFLDKIIKKRQYKFWLNDQEQYNYLKSLAQQNKIRALKGDLNGDTTVKG
                     IATAARQMGVPMRILYYSNAEEYFNGYKPPFKNSFSQMPVDDKSFVVRTISFNKGKFR
                     WAEDSDLSTDRGFHYSLQ"
BASE COUNT          556 a          449 c          466 g          475 t
ORIGIN      
        1 agggccgggg gtcgcgatcc gcatattgac ggcggtcacc aaagaaaata ttgccacgtt
       61 acgcgacgcc gatgcgattt tattagacga actcaaggcg tcggggtggt acgagaaagt
      121 ttggcaagcg ggcgcgattt ttttaccggt gcgctcggtc ggcgtcatgg gcgataaccg
      181 cacgtacgaa aacgtgattg cgctgcgctt ggtgacgagc gaggacggca tgaccgccga
      241 ctttgcccat atcccgtacg acgtgctttc aaaaattgct tcacgcatca ttaacgaagt
      301 acggggtgta aaccgtgtgg tctatgacat ctcttcaaaa ccgcccgcga cgattgagtg
      361 ggaatagggg agtgatggcg tgaggcgctt tatccgcatc tttttctttt tttgttgtac
      421 gcctatttat ttttctcttg cggcagattc gtggaaaaac gagtggcgcc aagaagagag
      481 tccttcaaaa gaaaaccgcg ataaatccaa aggctccgat cgcttggtta aagagctgga
      541 tgaagtatgg cgcgtggttt ttaagaatgc taaaggcgaa gacgtggtgc tcgactgcga
      601 agtagcgcgc agcgaaaaag acaaagaacg gggcctcatg ttccgtaaat ttttgggaaa
      661 aaatcgtggc atgatttttg tgtatgaaga gccgcacgaa atgcggtttt ggatgcaaaa
      721 cacagtattg ccactcagca tcgcctttat ccacagtaat ttatttatat cgagcttgca
      781 cgacatgaaa cccatgtcac tcgacattgt cgcttccgag gtgccaacac tctatgcgat
      841 tgaagcaaac caaggctggt ttaaaaccaa cacaattttg cccggcaatc ggctgactat
      901 ctacaaaaaa aaagacgagt acaaaatcaa aagaacgaat aaataccgcc aagctctccc
      961 cgaagtgaag tagtgttttg gaaatcgaca cgatcaccaa aaatctctca caaacaccgc
     1021 tgaaaaagcg ttttcgtgcc gtttcgtaaa tggttcatgc caaatgcgtc gaattttatt
     1081 actcgtggga ttttccccgt tacttctttt tctttattgt aaagaaaaaa cactaaacaa
     1141 acccgcagtc gagatacaaa agattttttg gtcctctccc gaagaagtct taaaacccac
     1201 tatttgggat actggcgata ccaccgtcac taacgaagat cgtttagaaa ttttccagcc
     1261 acacatcgcc ggtattggcg gcggttattt tggcgtgggt tcgattcaga actttactct
     1321 cgccgcatgg gcacgaagcg aatatatttg gctcatggat ttcacgcgcg tggtggtggc
     1381 agccaataaa attcacactg cgtttttgaa acgcttgagc ggccccgaag agcaccgcct
     1441 catgtgggac aaaaaaaagt cggcgacagc ggtggacaaa attatcgaca aagagtttaa
     1501 gaacgatccc gacttagctt ttattaaaca gagctaccgc aaagcgtcgc cgtatctcat
     1561 gatcacattc ggcttcttag ataagattat caaaaagaga caatataaat tttggctcaa
     1621 cgaccaagag caatataact atttgaaaag cttggcgcag cagaataaaa tccgagcgct
     1681 caagggcgac ttaaacggcg acaccaccgt gaagggaatt gccacagcgg cgcgtcaaat
     1741 gggggtgccg atgcgtattc tctattactc taacgccgag gagtatttca acggctacaa
     1801 acccccgttt aagaattcat tttcgcaaat gccggttgac gataaatcct tcgtggtacg
     1861 caccatatcc ttcaataaag gcaaattccg ctgggcagaa gattcggatt taagcaccga
     1921 ccgtggcttc cattacagcc tgcaac
//
LOCUS       DAOMPD010000509         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22790_length_1937_cov_2.565887, whole genome shotgun sequence.
ACCESSION   DAOMPD010000509 DAOMPD010000000
VERSION     DAOMPD010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22790_length_1937_cov_2.565887"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLY93_13480"
     CDS             complement(<1..469)
                     /locus_tag="PLY93_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP70534.1"
                     /translation="MIAFDVVVIGSGPAGEGAAMQCAKDGKRVAMVDLNPELGGECLH
                     RGTIPSKALRHSIQRFLESTTNPLVKVFQSSVAELNFPALVNAARSISRREAEINRRN
                     YDRNRVAIFVGYAKITSPNTITVALRDGGEEMLSAVAIIIATGSRPYRPQDIPF"
     gene            complement(466..1302)
                     /gene="lpxA"
                     /locus_tag="PLY93_13485"
     CDS             complement(466..1302)
                     /gene="lpxA"
                     /locus_tag="PLY93_13485"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRP70535.1"
                     /translation="MPKIHPTAVVENPTNLADDVVVHAMAYIGPDVKIGAGSEVGVRA
                     TITNRVTLGKNNKIFTGAALGEGAQDIHFDNPEAEIVIGDNNVFREFATVHQPTIKGK
                     KTTIGNGCYFMAVSHVAHDCHVGNNVIMVNKTGIAGHVEVGDNALISGLVMVHQFVRV
                     GAFAVLGGVSKATRDILPFTMNNGNPALAFGLNVVGLKRSGMSPAERSAIKQAFQIFY
                     LKDLSTPKAIDAIQNELLPSLPEGSPERKRIEYIGEWLKTSKRGFTFHASKRHEVENE
                     TE"
     gene            complement(1477..>1937)
                     /locus_tag="PLY93_13490"
     CDS             complement(1477..>1937)
                     /locus_tag="PLY93_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70536.1"
                     /translation="DFIGHAIRKFPNLSETDFILLTHRLELVPPRNYVYPVLEKPLRA
                     AQIRQVVMQRMKYAQDIVAMQARAETETTVTLEERPVDGDAPKKKGIRDLLKINRKTE
                     NNDGETEKAASTSTNKKALKSAKASTKTTKELAPKKSVSKTKTSKLKKKS"
BASE COUNT          456 a          469 c          466 g          546 t
ORIGIN      
        1 caaagggaat gtcttgggga cggtagggac gcgagccggt tgcgataatg atcgccaccg
       61 cgctcaacat ttcctcaccc ccatcgcgga gcgcaaccgt aatcgtgttg ggtgaagtga
      121 tttttgcata tcccacaaaa attgcgacac ggttgcggtc gtaattacga cggttaatct
      181 ccgcttcacg gcggctaatc gagcgtgcag cgttcacgag cgcagggaaa ttcaactcgg
      241 ctacgctgct ttggaatact ttgacaagag ggttagtcgt cgattctaaa aacctctgta
      301 tagaatggcg cagcgctttc gagggaatag tgcctcggtg caggcattcg ccgcccaatt
      361 ctgggttgag atcgaccatc gcgacacgtt taccgtcttt ggcacattgc atcgccgcgc
      421 cttcgcctgc ggggccagag ccaatgacaa cgacatcaaa agcgatcatt cggtttcgtt
      481 ctcgacttcg tggcgcttcg aggcgtggaa agtaaaaccg cgctttgagg tctttagcca
      541 ctcgccgata tattcgatgc gtttgcgctc gggtgagcct tcgggaagcg aggggagtaa
      601 ttcattttgg atggcgtcga tggctttggg agtcgaaagg tctttcaagt aaaaaatttg
      661 aaatgcttgt ttgatcgccg atcgttcggc gggagacatg cccgagcgtt tcaagcccac
      721 cacgtttaat ccaaacgcca aagcgggatt cccattgttc atagtaaaag gcagtatgtc
      781 gcgcgtggct ttactcaccc cacccaacac ggcaaacgca cccacgcgca cgaattggtg
      841 taccatcacc aaaccagaaa tgagcgcatt atcgcccacc tcgacgtgac cggcgatacc
      901 ggttttattc accatgatga cgttattacc aacgtggcaa tcgtgcgcaa catgcgaaac
      961 cgccataaaa taacatccat taccaatcgt cgtttttttt ccttttatcg tcggttgatg
     1021 cacggttgca aactcacgaa agacgttatt atcgccaatg acgatttcag cttcagggtt
     1081 atcaaagtga atatcctgtg cgccttcacc gagcgctgct cccgtaaaaa ttttattatt
     1141 tttgccgaga gtgacacggt ttgtaatagt cgcacgtacg ccaacctctg aaccagcgcc
     1201 aatttttaca tcggggccga tgtacgccat ggcgtgcaca acgacatcgt cggcgagatt
     1261 ggtgggattt tccacgaccg cagtggggtg tattttaggc atgtggtaat cccctattca
     1321 aaaataggcg gtcatgcgtt ttttatgacc gttaacctaa gagactgttc gccaactggc
     1381 tgagataatt ttcttacaac tttaatgtac acgctgccga gttgaagtac agactcccag
     1441 ccaaaatttg cgtagattta aaacaagggt aaacttttaa gattttttct ttaattttga
     1501 agtttttgtt tttgacacag atttttttgg ggctaattct ttcgttgtct ttgttgacgc
     1561 tttcgcgctt ttaagagctt tcttattcgt gctggtcgat gctgccttct cagtctcgcc
     1621 atcgttattt tcagttttac gatttatttt gagtaaatcg cgtatccctt tctttttagg
     1681 tgcatcacca tcgacaggtc tttcttccag cgttaccgtc gtttcagttt ctgcacgcgc
     1741 ctgcatcgcg acaatgtctt gtgcatactt catccgttgc atcacgactt gccgaatttg
     1801 cgctgcacgc aacggttttt ctaaaacagg atatacataa tttctaggtg gtaccagttc
     1861 caaacggtgg gtgagcaaaa taaaatcggt ttcggaaagg ttggggaact tgcggattgc
     1921 atggccgatg aaatcca
//
LOCUS       DAOMPD010000510         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22881_length_1932_cov_2.149707, whole genome shotgun sequence.
ACCESSION   DAOMPD010000510 DAOMPD010000000
VERSION     DAOMPD010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22881_length_1932_cov_2.149707"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLY93_13495"
     CDS             complement(<1..678)
                     /locus_tag="PLY93_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70537.1"
                     /translation="MIEAIYNHLHSPITFLWIDLLWVVFTILLLTLAILFYFLNRKAI
                     INRAFALLLLVIAYFAIISILTISFRVWEAQFIILIMPILCYLGFTFHLFIKAVIGSK
                     NLMERGDYGYFIVGFIFTFYTLYELLTPTAYEALRQTTSIIEHRVERAYTRHPMYILY
                     VFYIALPIFWATYQLIKTIRTPTEEQKEILPLAKNIFWIYLSSISLALLSLSVAPILG
                     FKQSPALG"
     gene            complement(700..1665)
                     /gene="fmt"
                     /locus_tag="PLY93_13500"
     CDS             complement(700..1665)
                     /gene="fmt"
                     /locus_tag="PLY93_13500"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRP70538.1"
                     /translation="MTEPQKTRVVFWGSPRIAAEFLTALTQHEKLSVIACVTQPEKIL
                     QRQGKTLTRSTVHERAAALEIPVFTPKLIKKEADELLHALNQIGFDIFVVLAYGKILP
                     QSIIDAPRLKAVNFHGSLLPLLRGASPIEHALLYGFKQTGWTLQRIVSQLDAGSIIAQ
                     SQVAIAEDETTQTLYAKMTADLLEHGIAMLMNYIEGRADIRAQDESQATHCGKILAED
                     GRLIFTESAHALRNRYRAFTPRPGVFAFFRGKKIKLTFNLTTPPKAMASAPGSLLQTG
                     KTELWLVCADGHALLLESVTPEGKKTISAADFINGYRVTTGDIFN"
     gene            complement(1658..>1932)
                     /locus_tag="PLY93_13505"
     CDS             complement(1658..>1932)
                     /locus_tag="PLY93_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRP70539.1"
                     /translation="AFQSRLGRGKWLTPMTSDTIAHLPTQGIKHLAVLAPSFVADNLE
                     TLHEIDIEYRHLFLSAGGKSFTYIPCLNDDTRWVKGFAAFIDKNYD"
BASE COUNT          514 a          402 c          510 g          506 t
ORIGIN      
        1 ccctaatgcg ggcgattgct taaaacctaa aatcggcgcg acggataaac ttaagagtgc
       61 aagcgaaatg cttgagagat aaatccaaaa aatatttttt gctaaaggca aaatttcttt
      121 ttgttcttcg gtgggggtgc gtatcgtttt aataagttga tacgtcgccc aaaagatagg
      181 aagcgcaata taaaacacgt agagaatgta catggggtgt ctggtgtacg cccgttcaac
      241 acggtgctct ataatcgatg tcgtttgtcg tagcgcttcg tatgcagtgg gggtgagtag
      301 ttcatagagt gtgtaaaaag taaaaataaa gcctacaata aaatatccgt aatcgccgcg
      361 ttccatgaga ttctttgagc cgataaccgc cttgataaag agatgaaacg taaatcctaa
      421 gtagcataaa atcggcatga tgagaataat aaattgcgct tcccaaacac gaaatgaaat
      481 ggtgagaata ctgatgatgg caaaataggc aatgactaaa agcagcaacg cgaaagcacg
      541 gttgatgatg gcttttctgt tgagaaaata gaagagaatt gcaagagtga gaagtaaaat
      601 agtgaaaaca acccagagta aatcaatcca caggaaggtg atgggactgt gcaggtggtt
      661 gtagatggct tcaatcatgc cgttaaggat attcttattt tagttaaaaa tgtctcccgt
      721 ggttacacga tacccgttaa tgaaatccgc cgcgctaatc gttttttttc cttcgggggt
      781 tacgctttca agaagaagcg cgtggccgtc tgcgcagaca agccaaagct ctgtttttcc
      841 tgtttgtaaa agacttccag gagcgcttgc catggcttta ggtggtgtcg ttaaattaaa
      901 cgtcagtttt attttcttgc cacggaaaaa agcaaacacc cccgggcgcg gcgtgaaggc
      961 gcggtagcgg tttcgcagcg cgtgcgcgct ttcggtaaag atgagccttc cgtcttcggc
     1021 taaaatttta ccgcaatgcg tcgcttggct ttcgtcttga gcgcggatgt cggcgcggcc
     1081 ttcgatgtaa ttcatgagca ttgcgatacc atgctccaat aaatctgccg tcattttggc
     1141 gtaaagcgtt tgcgtcgttt catcctctgc aatggcaact tggctttgcg cgataatact
     1201 gccagcgtcg agttgggaaa caatccgttg cagcgtccag ccggtttgct tgaagccata
     1261 gaggagtgca tgttcaatcg gcgatgcgcc ccgcaggaga ggtaagaggc taccgtggaa
     1321 gttgaccgct ttgaggcgcg gcgcatcgat gatgctttgt ggcaaaattt ttccgtacgc
     1381 caacaccaca aaaatatcaa aaccaatttg attgagtgcg tgcaagagtt catccgcttc
     1441 tttcttgatt aactttgggg taaagacggg aatctctaac gctgcggcgc gttcgtgtac
     1501 cgttgaacga gtgagtgttt ttccttgtcg ttgcaaaatt ttttcaggtt gggtgacgca
     1561 cgcgatcact gataattttt catgctgcgt gagagccgtc aaaaactcgg ctgcgatgcg
     1621 gggcgagccc caaaaaacaa cgcgcgtttt ttgaggctca gtcataattt ttgtcgatga
     1681 atgcggcaaa gcctttcacc cagcgggtgt cgtcgttgag gcagggaata tatgtgaacg
     1741 attttccccc cgcagataga aaaaggtggc ggtactcgat gtcgatttca tggagtgtct
     1801 ctaaattatc ggcgacaaaa gacggcgcaa gtaccgcaag atgtttgatc ccctgcgtgg
     1861 gtaagtgagc gatggtgtcg ctcgtcatgg gggtgagcca tttaccgcgc cccaagcgcg
     1921 attggaaagc gc
//
LOCUS       DAOMPD010000511         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22926_length_1928_cov_2.804058, whole genome shotgun sequence.
ACCESSION   DAOMPD010000511 DAOMPD010000000
VERSION     DAOMPD010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22926_length_1928_cov_2.804058"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLY93_13510"
     CDS             complement(<1..651)
                     /locus_tag="PLY93_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70540.1"
                     /translation="MKKLKLILPIVCLALNTFACKSASEKYEATATDNRVRVTDDWVK
                     ATGVAAIRDGNMGLAKDAKRVAVQEVLGSMISARSEMQNFQLISQKVTSQSEGMIEKF
                     EVLSASAVSAVEYQVKIRAKVSTALIDKTIEETVSAQGKPRMMVLIDETIEGEADNNK
                     TARSAIEGEFSNQGFPMVDPDTVEKLIAKNKKKIAQALDGNNTAATELGVDAGAEII"
     gene            complement(673..1248)
                     /locus_tag="PLY93_13515"
     CDS             complement(673..1248)
                     /locus_tag="PLY93_13515"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRP70541.1"
                     /translation="MKKKYVWFLPLFIASCIITGKNSDLLDPVNAGLNLRDPGNGTII
                     DTVTKLVWKRCSQGQSDDAACSLTAATFQYCTSDDNACNGGVDTATLESGPAFDSCNA
                     LNSNPSGGYAGYTTWRVPTYDELWTIHVNARETSEAGLNNLFPNLDKAVYYLSATSAS
                     YNEYRQVLYKPPVANHTATKTTPSALRCVAQ"
     gene            complement(1259..1894)
                     /locus_tag="PLY93_13520"
     CDS             complement(1259..1894)
                     /locus_tag="PLY93_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70542.1"
                     /translation="MSRGSTKEAEEEKYEKNLDDSIYARRVDNVVRRSTLRLGVSGMG
                     LNQLGDEKNANSYFGDKALAGYIDIFGPGRNDIFSSGIGFIFRHMEMKDRHDLAGVGV
                     LDILGFNVAMRFFLLHFDFLWSIWSPYIIIDTRIVYVESTTISTGPDKKLGSLASFAS
                     LGVSARAGVQWSIAETFGFYAEALYGYTPVGKRETNIDGLQIVAGMLLRFK"
BASE COUNT          459 a          479 c          436 g          554 t
ORIGIN      
        1 aataatttct gcccctgcgt cgacgcccaa ctcggtggcg gcggtattgt tgccatccaa
       61 cgcttgcgct atttttttct tatttttggc gattaacttt tctacagtgt caggatcaac
      121 catgggaaac ccttgatttg aaaactcccc ctcgattgcc gatcgtgctg ttttattgtt
      181 gtctgcttcg ccttcaatag tctcgtcgat gagaaccatc atgcgcggct taccctgtgc
      241 tgaaaccgtt tcttcgattg ttttatcgat gagagccgtg ctcactttcg cgcgaatttt
      301 tacttggtat tcaacggcag aaaccgcaga tgccgagagc acctcaaatt tctcaatcat
      361 gccttcggat tgactcgtga ctttttggct aatgagttga aaattctgca tctccgaacg
      421 cgcagaaatc atacttccca acacttcttg cacggcgaca cgttttgcat cttttgcaag
      481 tcccatgttg ccgtcccgaa tagccgcgac gccggtggct ttcacccagt cgtcggtaac
      541 tctcactcga ttgtcggtag cggttgcctc atatttttca gaagctgatt tgcaagcaaa
      601 agtatttaga gcaagacata caataggtag tataagtttt aattttttca ttttattcct
      661 cattatattt ttttattgcg ctacgcagcg taacgcgctt ggagtggtct ttgtcgctgt
      721 gtgattagcc acaggcggct tatagagcac ttgtcgatat tcattgtacg acgccgaagt
      781 agcactgaga taataaacgg ctttatctaa gttgggaaaa aggttgttca accccgcttc
      841 gctggtctcg cgtgcgttca catgaattgt ccacagctcg tcgtatgtcg gtacgcgcca
      901 cgttgtgtat ccggcataac cgccgctggg gtttgaattc agtgcgttac aactgtcgaa
      961 tgcaggtccg ctttcaagcg tggccgtatc gacaccaccg ttgcaagcgt tgtcgtcgct
     1021 ggtacagtac tgaaaagtcg cagctgttaa gctacacgcc gcatcgtcgc tctgtccctg
     1081 cgagcagcgt ttccaaacaa gtttggtgac ggtgtcgata atcgtgccgt taccggggtc
     1141 tcgtaaattg agccccgcgt tcacggggtc gagtaaatca gagtttttgc ccgtgatgat
     1201 gcacgaagct ataaagagtg gtagaaacca tacgtatttt tttttcataa aatttacctc
     1261 acttaaaacg gagaagcatc cccgcgacaa tttgtaagcc gtcgatgttt gtctctcttt
     1321 taccgacggg tgtgtagccg tagagtgcct cggcgtaaaa tccaaatgtt tcagcaatcg
     1381 accactgcac ccctgcgcgg gcagagacac ccagcgatgc aaaggatgct aaactgccca
     1441 atttcttatc agggccggtt gagattgtcg tcgattctac gtagacaatg cgcgtatcga
     1501 taattatata tggactccaa atgctccaca gaaaatcaaa gtgcaataga aaaaagcgca
     1561 tcgctacgtt aaagcccaag atatccagca cgcccacgcc tgcgagatcg tggcgatctt
     1621 tcatttccat gtggcgaaaa atgaaaccta tacccgatga aaaaatatcg tttcgacctg
     1681 ggccaaaaat gtctatgtat cctgcaagcg ctttgtcgcc aaaatatgag ttagcgtttt
     1741 tttcgtcacc cagttgattt aagcccatac ctgacacacc aagacgtaac gtcgatcgac
     1801 gtacgacgtt gtcgacacgg cgggcataaa tcgaatcatc gagatttttc tcgtactttt
     1861 cttcttctgc ttcttttgta gaaccccgag acatcggtga cagctatgca gccaattttg
     1921 ctggtcgc
//
LOCUS       DAOMPD010000512         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_22967_length_1925_cov_2.958824, whole genome shotgun sequence.
ACCESSION   DAOMPD010000512 DAOMPD010000000
VERSION     DAOMPD010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22967_length_1925_cov_2.958824"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLY93_13525"
     CDS             complement(<1..723)
                     /locus_tag="PLY93_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRP70543.1"
                     /translation="MLVPSPAPEINELDELKNELKSEYKYGFVTDIESETIPVGLNED
                     VVRRISAMKNEPEYMLEWRLKAYRAWTQMKEPRWAEVSFPPIDYQKVSYFSRPKKKID
                     SLEDADPKLLETFKKLGLPLSEQMHLAGVKGSEVNTRPGIPGSTLGGMIPPNERPNVA
                     MDVVFDSVSVVTTFQDQLAKHGIIFCSFSEATRKHPELVQKYLGSVVPIRDNFFAALN
                     SAVFSDGTFVYIPPGVRCPMELS"
     gene            complement(842..1780)
                     /locus_tag="PLY93_13530"
     CDS             complement(842..1780)
                     /locus_tag="PLY93_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70544.1"
                     /translation="MVQRAFSAWVVIFYFFSCPSLQSQPPQKHVETVMPKEKKENPPP
                     TVVIQNALLNPYESEIFSENCQQEYDNFGTQKREMRYKKKKFRGSVVEQRSGRKRLYD
                     HNIFLRSLFAGGVTFNGASLDKKFDGALFLDIGSAILFGEGAVTVRDLYEDAAINTHL
                     TIIASDINDASNKSNRYIAIYRNKKNKLPFPVVEVEKKILSPKDFLRPLRLYFKSDDQ
                     PVILRSTNSGPDLYYDVPQAFLHLRSATAAFFTRDVLYLFNKYILYKPKRALGFIILG
                     EIDAAVGVNHRASPWENIDWDKRTFDDAVKLNRYYK"
BASE COUNT          493 a          407 c          486 g          539 t
ORIGIN      
        1 cgagagttcc atggggcaac gcacgcctgg ggggatatac acaaacgtac cgtcgctaaa
       61 aacggcggaa ttgagcgcgg cgaaaaaatt atcgcggata ggcacaacgg atcccaaata
      121 tttctgcaca agttcggggt gtttgcgcgt tgcttctgag aacgagcaga agataatacc
      181 atgctttgct agttgatctt ggaaagtggt gacgacggaa acgctgtcaa agacgacgtc
      241 catcgcaacg ttgggacgct cgttgggggg gatcataccg cccagcgtgc taccggggat
      301 tcccggacgc gtgttgactt cagaaccttt tacgccggct aagtgcattt gttctgagag
      361 gggcaatccc aattttttga atgtctcgag gagcttgggg tcggcgtctt ccaaggaatc
      421 aatctttttt ttaggccgtg agaaatagct cactttttgg taatcaatcg gtggaaagct
      481 tacctctgcc caccgcggtt ctttcatttg tgtccaagca cggtacgcct tgaggcgcca
      541 ctcgagcatg tattcaggtt cgtttttcat cgcacttatg cggcggacga catcttcgtt
      601 gagacccacg gggatggttt ccgattcaat gtcggtaaca aagccgtatt tatattcgct
      661 cttgagctca tttttgagtt cgtcgagttc gtttatttct ggcgcgggtg aaggaactaa
      721 catttgagga taatatacat aattgagaca attttgcaaa aaaaaatacc tttaagtcta
      781 cttcttgtca gtggattatg attgccactg tgtctctgga gagtgctcgt gcttattcct
      841 ctcatttata gtaacgattg agcttaactg cgtcgtcaaa ggtgcgcttg tcccaatcga
      901 tgttttccca cggcgaggcg cggtggttta cccccaccgc agcgtcgatt tcacccaaga
      961 taataaatcc caaagcgcgt ttcggtttat ataaaatgta tttattaaat aagtaaagca
     1021 catcgcgtgt aaaaaacgct gccgtggcag agcgtaagtg cagaaatgct tgcggcacgt
     1081 cgtaatagag atcggggcct gaattcgtgc tgcgtaaaat aaccggctga tcgtcgcttt
     1141 taaaataaag gcgcaacggg cgtaaaaaat cttttgggga gagtattttc ttttcgactt
     1201 caaccacagg gaacggtaat ttattttttt tattgcggta aatagcgatg tagcgattgc
     1261 tcttattgct tgcatcgttg atgtcgctgg caataattgt aaggtgggtg ttaatcgctg
     1321 cgtcttcgta taaatcgcgc accgtcaccg cgccttcgcc aaaaagaatg gcgctgccga
     1381 tgtcgagaaa aagcgcaccg tcgaattttt tatcgagact tgcgccgttg aatgtgacgc
     1441 cgccagcaaa aagcgagcgc aagaaaatat tgtgatcgta gagacgctta cgccccgagc
     1501 gttgttcgac gacgctgcct ctaaattttt ttttcttata gcgcatctcc cgtttttgag
     1561 taccaaaatt atcgtattct tgttggcaat tttcagaaaa aatttcgctt tcatagggat
     1621 tgaggagcgc attttggata acaaccgtag gcggcggatt ttcttttttc tctttaggca
     1681 ttaccgtttc aacgtgcttt tgcggtggtt gcgattgaag cgaagggcaa gaaaaaaaat
     1741 aaaaaataac gacccaagca gaaaatgcgc gctgaaccaa aagacatatt gcatgcaagg
     1801 ggcgatggcg gcgcatcaac gacaagactg agtatggcaa tgtgctttca aggtgatttt
     1861 taggttaagg ttatgcaacc cacagagata actgacactt ttggggtcaa gttgagtgcg
     1921 tttta
//
LOCUS       DAOMPD010000513         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23029_length_1920_cov_2.648257, whole genome shotgun sequence.
ACCESSION   DAOMPD010000513 DAOMPD010000000
VERSION     DAOMPD010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23029_length_1920_cov_2.648257"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLY93_13535"
     CDS             complement(<1..328)
                     /locus_tag="PLY93_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70545.1"
                     /translation="MPEKQKNLLDETVKQATQWGLRTLGAFAGSEFAQRTGLQAVARD
                     ALYGVTRTVVRAGTSILAAAKSATNLVGYPRLEKPKSSSDLFDLNFSEEQNMIAEAMG
                     EFAEGVL"
     gene            complement(418..951)
                     /locus_tag="PLY93_13540"
     CDS             complement(418..951)
                     /locus_tag="PLY93_13540"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 family protein"
                     /protein_id="HRP70546.1"
                     /translation="MKIFFRNLLIAYLLTFSGYMLLHVIGFNTTDHNKGDIARTSLVV
                     ISWCMIFVTIVQMRALETETHHFLTAFRYGFLFAVFFSLGFTLFLVLYQHVINPKFYS
                     TYRTYFENRLLTAQISAETLSTKMRQFDMSYDGKFPSYLLFFLSNGMGGIVTAAIAAA
                     IFREKKQKGDNYPHKSE"
     gene            complement(953..>1423)
                     /locus_tag="PLY93_13545"
     CDS             complement(953..>1423)
                     /locus_tag="PLY93_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRP70547.1"
                     /translation="SPYYFDLKQTLLNPQQLSLAGKIIATMVRQNYPSAVVAAGLTMG
                     ADPIIYAACLSEINISHPIFPAVVRKEVKDHGTGKRIEMLKSITPATPCVMVDDVITT
                     AGSTLKAVHAMREAGFTVRHAYCILDRAEGGREALAHEGIELLPVFTAAEFREK"
     assembly_gap    1424..1438
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1677..>1920
                     /gene="rpmA"
                     /locus_tag="PLY93_13550"
     CDS             1677..>1920
                     /gene="rpmA"
                     /locus_tag="PLY93_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRP70548.1"
                     /translation="MAHKKGGGSSKNGRDSNAQRLGIKAYGGEFVTAGSILVRQHGTV
                     FHAGRNVLRAKDDSLFTTIPGIVKFERIDKHRQKISV"
BASE COUNT          532 a          428 c          417 g          528 t
ORIGIN      
        1 tcagtacacc ttcggcgaac tcacccatcg cttcggcaat catattttgt tcttcgctaa
       61 agttgaggtc gaataggtcg ctgctgcttt tcggtttttc taaacgggga taccccacaa
      121 ggttggttgc actttttgcg gcagcaagaa tcgacgtgcc cgcccgcacc acggtgcgcg
      181 tgaccccata gagggcatcg cgtgcaacgg cttgcaagcc tgttctttgt gcaaattcag
      241 aaccggcaaa cgcgccgagg gtacgcaatc cccattgcgt cgcttgcttc accgtttcgt
      301 ctaaaaggtt tttttgtttt tctggcatag ttatccctat ctaagaaaaa gataaagaac
      361 cagagggtcg aatacagcgt caataaaaat tgaacatttg ttcaagtttt gaggttctta
      421 ttcacttttg tgaggatagt tatccccttt ttgctttttt tctctaaaaa ttgccgcagc
      481 gatggccgct gtaacgatgc cacccatgcc gttggagaga aaaaataata agtaagaagg
      541 aaatttacca tcatagctca tatcaaactg gcgcatcttc gtcgataacg tttctgcgct
      601 gatttgtgcg gtgagcaagc gattctcaaa atatgttcga tacgtggaat aaaacttggg
      661 attgatgacg tgttgatata acaccagaaa aagagtgaac cccaaagaaa aaaatactgc
      721 aaacaaaaaa ccatagcgaa acgccgtcaa aaaatgatgt gtctcagttt caagagcacg
      781 catttgtaca atcgtcacaa aaatcataca ccacgagata acaactaagg acgtacgcgc
      841 tatgtcccct ttgttatggt ctgttgtgtt gaagcctatc acatgcaaaa gcatgtatcc
      901 gctgaaagtc agtaaataag caatgagtaa gttacgaaaa aatattttca ttttattttt
      961 ctctaaattc ggcggcggta aagacaggca acagttcaat gccttcgtgc gctaatgctt
     1021 cacgtccccc ttcggcacgg tctaaaatac agtatgcatg tctcacagta aaacccgctt
     1081 cgcgcattgc atgcactgct ttgagagttg agcctgctgt ggtaattacg tcgtcgacca
     1141 tcacgcaagg tgttgcggga gtaatacttt tgagcatttc aattcttttt cctgtgccgt
     1201 ggtctttcac ttctttgcgc acaaccgctg gaaaaattgg gtgggatata tttatttcgc
     1261 tcaagcacgc cgcgtaaata attggatcgg cgcccatcgt aagacctgcg gcaacgactg
     1321 cgcttggata attctggcgc accattgttg caataatttt acccgccaga gacaattgtt
     1381 gtgggttcag tagtgtttgc ttgagatcaa agtaataggg actnnnnnnn nnnnnnnngg
     1441 ttttcgcggt aagacagtgt gcggatcgcg ttctttaatg cttctttatc agtggctatg
     1501 tgaggcatac tggcatctcc aaaaagagaa acagatggca aagctaacta taccctgtgt
     1561 acaaaatgag catacattag agcctcgtac gagttagcgc acaggctcct ccttgtattt
     1621 tcatataaac caacaaatag actttacgac cagtttcaac cgatagaaat gacaatatgg
     1681 cacacaaaaa gggcggcggt tcgtctaaaa acggtcgcga ttcaaatgca caacgcttag
     1741 gtattaaagc ttatggcggc gaatttgtca ctgcagggtc tatcctcgtt cgtcagcacg
     1801 gtacagtatt tcatgcaggt cgcaacgtct tgcgtgcaaa agacgattca ctttttacga
     1861 ccatccctgg catcgttaaa tttgaacgta tcgataaaca ccgccaaaaa atctctgttt
//
LOCUS       DAOMPD010000514         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23088_length_1916_cov_2.641591, whole genome shotgun sequence.
ACCESSION   DAOMPD010000514 DAOMPD010000000
VERSION     DAOMPD010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23088_length_1916_cov_2.641591"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..870)
                     /locus_tag="PLY93_13555"
     CDS             complement(616..870)
                     /locus_tag="PLY93_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70549.1"
                     /translation="MEKKIKIVVHTKDNITNESALRNSDWKMTMPNTELGDRWEKDAM
                     LDPNDQAKAEKLFGAAVWDFYNAAGRNKPNIHMAITDLIN"
     gene            1416..>1916
                     /locus_tag="PLY93_13560"
     CDS             1416..>1916
                     /locus_tag="PLY93_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRP70550.1"
                     /translation="MKLFVLVFSFFLIATTTYALESDAEQFDIAAFRQSVEKRIQKRT
                     LKNGLRVIVMRNTANPTVACYLKIGVGSANESFDQAGTAHFLEHLLFKGTPTLGTTNF
                     EAEKPFLEQMIVDGERIDTLERTLLNPLLSSDERKKIEARKRVLEKRLLRWQKGAQQY
                     VLSEEDS"
BASE COUNT          493 a          451 c          472 g          500 t
ORIGIN      
        1 cgcatgttgg gcgaaattgg cgtttggtgc gtcgttgaat aatttaccgt cgcttgccgg
       61 tagttgaacg agtcgccggc gtccgtgccg gctcttcctt cgcagcatcc ttgctgctcc
      121 gggtttatgc cgttctgaat atttacaaca acagcaaaag aaatgtggaa agtgcaaaac
      181 cgcccgactt ttcttttagc cgagaaataa tttttgcggt tttacacatt ccacatcgtt
      241 ggttttgttt ggagaaaata tttttccgcc gccccgcagc gctgggaaga tatattttag
      301 cgtaggtgcg cataataggt tttttatgtc ggtgcgcctg cctgtggcag gccgaagggc
      361 gcagcttgcc gaaagcatca acgtaggcaa gctacgccgc acgaacttca cgacccacgg
      421 agcatatttt ctttcagaga aaagcgtttt ggagtgaagc ggcataaaaa aagttatgcg
      481 caccgaagcg ttgggtaaaa cggcacggtg ttgcgagtag ccgtcggcca actgttagcc
      541 ggttcggctc ccagccgacg ggaggccgca ggccgattgg caacgccgtg gagcgctgcg
      601 gggcggcggt gggggctagt tgatcagatc agtaatagcc atgtggatat ttggcttatt
      661 tcgacctgct gcattgtaga aatcccaaac cgcagcgcca aagagttttt ccgctttggc
      721 ttgatcgttt ggatcaagca tggcgtcttt ttcccagcgg tcacccaatt cagtgttggg
      781 catcgtcatt ttccaatctg aatttcgcaa agcactttcg tttgtaatgt tatcctttgt
      841 gtggacaaca attttgatct ttttttccat atctataaaa tacgagaact gggctaagta
      901 tcgtaaataa aattcaggat gatggtttcg ccaacgggta gcgatgcggt cactggtgag
      961 acatcattaa attcgtgttt ttgaccgttg aattgaatga tgatgattat gaagctgcga
     1021 ctatttcaag tagagtacca tagtacgcca tgcccaccct tgtcaccgac tcacgcgcca
     1081 acatttgcca gccccacccc ctttccccct ccccacgacg tagggagggg gatgtgattc
     1141 caccgttgct gggcgtggag gtgaacttgc cacggttcaa acatcttgca atctgacgaa
     1201 attacgtaca tctaatgttg cttgcctcca cacaaataaa gataaaaaga ccaaaaaagt
     1261 agacgtaaaa cgtccttatg cgcagctatc taaaatgttt caacgtaagg caaaggaaga
     1321 acagaactca ttatgcggta taaagagcct gtgcgccaac aatcttaata attagatttt
     1381 tatcgccgcg acgcggcgga accttaaact ggtatatgaa acttttcgtt ctcgtctttt
     1441 cgtttttcct tatagcaacc acgacgtatg ctcttgagag tgacgcagaa caattcgaca
     1501 tcgccgcttt tcggcagagt gtggaaaagc gtatccaaaa gcgcacactt aaaaatggac
     1561 tccgcgtaat tgtgatgcgc aacactgcca atcccacggt ggcgtgttat ctgaaaatcg
     1621 gcgtgggctc cgcgaatgaa tcgttcgacc aagcgggtac cgcgcatttt ttggagcacc
     1681 tccttttcaa aggaactccc acgttgggca cgaccaactt tgaagcagaa aaaccgtttt
     1741 tagaacaaat gattgtcgat ggcgagcgca ttgacacact cgaacggact ctgctcaatc
     1801 cacttttaag tagcgacgag cgtaagaaaa ttgaagcgcg taaaagggtt ctagaaaagc
     1861 gtctcttgcg ttggcaaaaa ggcgcgcaac aatatgtcct ttccgaagaa gactcg
//
LOCUS       DAOMPD010000515         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23108_length_1915_cov_1.890860, whole genome shotgun sequence.
ACCESSION   DAOMPD010000515 DAOMPD010000000
VERSION     DAOMPD010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23108_length_1915_cov_1.890860"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLY93_13565"
     CDS             complement(<1..1004)
                     /locus_tag="PLY93_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP70551.1"
                     /translation="MNLEIPKKFNQITELAQQVAEHTFRPVSRKYDRGEHNYPVELDM
                     FDAILRGMEESGTGFGASGATKDKKTNDTGEVRNGKNMQLVLNTMEMCRGDAGLLLTI
                     PGQGLGNAAIAAVANEEQKARFGGKWAAMAITEPEAGSDSSAIRTTARLDGDEWVING
                     EKIFVTCGERAKVVVVWATLDRSLGKAAIKSFVVEHGTPGMRVERLERKLGIRASDTA
                     TIVFTDCRIPRSNLLGTEAIETEQSFVGAMKTFDNTRPLIAAMALGVARASLDLTTEL
                     FEKAGVKLPYTKASLDVSAIEAELYRLEAEYEAARLLALKAAWMADNGQANSLEASMA
                     "
     gene            complement(1014..>1915)
                     /locus_tag="PLY93_13570"
     CDS             complement(1014..>1915)
                     /locus_tag="PLY93_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRP70552.1"
                     /translation="RNPMTGPLIAEKLGQGDYALALRILSSISFVNLLNDYGSALQRS
                     AFLPLFTGDKYMQASLAIMEPQLRFDIHKLKTTAERSHSGYWLNGEKTMVVAAKDADV
                     FLIFADLTGVGIHPFIVEKNTEGVIIEEERHMGLLAASMAKVKLQNVHVPKEALLGES
                     ESSFDLEKFIDASRIALGALTVGTMKSAIDYAITYCNQRVAFGEPITNRQAVAFMLAD
                     MATEYEALRLMVYRAAALFEQGKDCRRAAYLADVFSAQYSMRIATDAVQLLGGHGYTR
                     EHPVEMWYRHLRASAILRGTISL"
BASE COUNT          413 a          527 c          498 g          477 t
ORIGIN      
        1 ttcgccatcg acgcttcgag cgagttggct tggccgttgt cggccatcca cgccgccttg
       61 agagcaagaa gtcgagctgc ttcgtattcc gcttcgagtc gataaagttc ggcttcaatc
      121 gcactcacat ccagagatgc tttggtgtaa gggagtttta cgcccgcctt ttcaaaaagc
      181 tcggtggtga ggtctaacga cgcgcgcgcc acacccaacg ccatcgctgc gatgagcggg
      241 cgggtgttgt cgaaagtttt catcgcgccg acaaagctct gctcggtttc aatcgcctct
      301 gtacccaaaa gattcgagcg cgggatgcgg caatcggtga acacaatcgt cgcagtatcc
      361 gacgcgcgga tgcccagctt tctttctaaa cgctccacgc gcatgccggg agtgccgtgt
      421 tcgacgacga acgatttaat cgccgctttg ccaagcgagc ggtcgagagt cgcccacacc
      481 acaaccacct tggcgcgttc gccacaggtg acgaaaattt tttcaccgtt gatgacccat
      541 tcgtcgccgt ctaagcgcgc ggtggtgcgg atggcgctcg aatctgagcc ggcttctggt
      601 tcggtgattg ccatcgccgc ccacttgcca ccgaaacgag ctttctgttc ttcattggcg
      661 acagcggcaa tcgctgcatt ccccaagcct tggccgggta tcgtgagcaa taaccccgca
      721 tcgccccgac acatttccat cgtgtttaac acaagctgca tatttttgcc gttacgcact
      781 tcgccggtgt cgttggtttt tttatctttg gttgcgcccg atgcaccaaa gccggtgccg
      841 ctttcttcca tgccgcgcaa tatggcgtcg aacatatcga gctcaaccgg atagttatgc
      901 tcaccgcggt cgtatttgcg cgaaacaggg cggaaggtat gctctgcaac ctgttgcgct
      961 aactctgtaa tttggttaaa ttttttaggt atttcaagat tcatggttga cctctataaa
     1021 gaaattgtac cgcgtagaat cgcgctcgct ctcagatggc gataccacat ctccaccgga
     1081 tgttcgcggg tatagccgtg gccgcccaag agttgcaccg cgtcggtcgc gatgcgcatt
     1141 gaatattgcg cgctgaacac gtcggcaagg tatgccgcgc ggcggcaatc tttgccttgt
     1201 tcgaaaagtg cggcagcgcg gtacaccatg aggcgcaacg cttcgtactc ggtggccatg
     1261 tctgcgagca tgaatgccac ggcttgccgg ttggtgatgg gttctccaaa tgcaacgcgc
     1321 tggttgcagt aggtgatcgc gtaatcaatc gcgcttttca tggtgccgac ggtgagagct
     1381 cccaatgcga tgcgacttgc atcgatgaat ttttctaaat caaaagaaga ttcactttca
     1441 cccaaaagcg cttctttcgg tacgtgtacg ttttgcagct tcactttcgc catcgatgca
     1501 gccaaaaggc ccatgtggcg ttcttcttcg atgataaccc cttctgtatt tttttcaacg
     1561 ataaagggat ggataccaac accggttaag tcagcgaaga taagaaatac atccgcatcc
     1621 tttgcggcaa cgaccattgt tttttcgccg ttgagccaat agccgctgtg gctgcgctcg
     1681 gccgttgttt taagtttgtg gatgtcaaaa cgcaactgcg gctccatgat tgcgagcgat
     1741 gcctgcatat atttatcgcc agtaaagagc ggcaggaatg cagaacgctg caacgcgctg
     1801 ccataatcgt tgagcaaatt tacaaaggaa atcgacgata aaatccgcaa cgccaacgca
     1861 taatcaccct gacccaattt ctcagcgatg agcggtcccg tcatggggtt acgtt
//
LOCUS       DAOMPD010000516         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23148_length_1912_cov_3.362951, whole genome shotgun sequence.
ACCESSION   DAOMPD010000516 DAOMPD010000000
VERSION     DAOMPD010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23148_length_1912_cov_3.362951"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLY93_13575"
     CDS             <1..486
                     /locus_tag="PLY93_13575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRP70553.1"
                     /translation="STTLDELREKVQRVSGLSHIFISRHAAKGLVKIPHEYTPQFTPK
                     LENFSLLNHETVWALSAIANSRAFEEMLRRAGAEKVEQISFRDHHDFSEKDIRMVLSR
                     IKPYDIVVTTEKDYVRLRKHAQALQVLKQFYYLKIEFEVPENEILLKEGLKAHLLKNQ
                     S"
     gene            complement(470..1228)
                     /locus_tag="PLY93_13580"
     CDS             complement(470..1228)
                     /locus_tag="PLY93_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70554.1"
                     /translation="MKLVSLFALLIMGGFTSLLTAGPEIYDPLDAYKKSNTLSSKPQS
                     EIVTQGTTVESARFKYDAGLLARVDYLDSKKNLTGYSLFDYDKGILVRERLFHIGDIL
                     VEDIKYEYHKNKLVKSLAHDIRGNALIEWRYVYDKEGQLVGGKRFLAKHLTESFRVTK
                     NANGTTQHIYNARGELTSKVESVYKDGLLMHRLKTGLVGARYADYRYNTSKELVEIIY
                     HETVRGEKKWVKTHRFEYSMAANVPTRSAFKTGS"
     gene            1321..1521
                     /locus_tag="PLY93_13585"
     CDS             1321..1521
                     /locus_tag="PLY93_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70555.1"
                     /translation="MQGIQEIGIASVAQPTVISSEDLKALLYLTIRGHNGLTGAAVEE
                     KNSVRTTEDRGIDFEKGVDVRA"
     gene            1555..1725
                     /locus_tag="PLY93_13590"
     CDS             1555..1725
                     /locus_tag="PLY93_13590"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70556.1"
                     /translation="MESMTGYGESHFNAEGISIVCSARSLNSRFLEVELDYPSELLWF
                     KAASEAIIQRRF"
BASE COUNT          527 a          419 c          439 g          527 t
ORIGIN      
        1 agcacaacgc tcgacgagtt acgtgaaaaa gtgcaaagag tgtctgggtt gtctcacatt
       61 tttattagcc gccacgccgc caaaggactt gtgaaaattc cgcacgagta cacaccgcaa
      121 tttaccccca agcttgaaaa tttttcactc ttaaaccacg agaccgtgtg ggcgctttcg
      181 gcgattgcca acagtcgcgc gtttgaagaa atgctgcggc gtgcaggcgc ggaaaaagta
      241 gaacaaatta gttttcgcga tcaccacgat ttttcagaaa aagacatccg catggtgttg
      301 agccgcataa aaccatacga cattgtcgtc acaaccgaaa aagattatgt gcgcttgcgc
      361 aaacatgcgc aagcattgca agtgttgaag cagttttatt atttaaagat tgagttcgag
      421 gtgcccgaga acgaaatttt attgaaagaa ggcttaaaag cgcacttact caagaaccag
      481 tcttaaatgc ggatcgtgtc ggtacgttgg cagccatcga atattcaaag cggtgtgtct
      541 tcacccattt cttttcaccg cgcacggttt cgtgataaat aatttcaacc aactctttcg
      601 acgtattgta gcggtaatcg gcgtaacgcg cacccacaag accggtcttt agtcggtgca
      661 tcaataaacc gtctttatat accgactcaa ctttgcttgt tagttcaccg cgcgcattgt
      721 aaatgtgttg cgttgttccg ttcgcgttct ttgtcactcg aaaagactcc gtcaaatgct
      781 tggcgagaaa gcgtttgcca ccgacgagct gtccttcttt atcgtataca taacgccatt
      841 caatgagtgc gtttccacgg atgtcgtgtg ccaatgattt aacgagtttg tttttatgat
      901 attcgtattt aatgtcttcg acaagtatat cgccgatgtg gaaaagacgt tcgcgcacaa
      961 gaattccttt atcgtaatcg aacaaagaat aacccgtgag atttttttta gaatcgagat
     1021 aatcgactct cgctaaaaga ccggcatcgt acttgaaacg cgcgctttcc acagtggtgc
     1081 cttgagtgac aatttcactc tgtggcttcg acgatagtgt gttactcttt ttgtaagcgt
     1141 cgaggggatc gtatatctca gggcctgccg ttaaaagaga agtgaatcca cccatgatga
     1201 gcaaagcaaa tagtgaaacg agcttcatag aatgtatttc gacgcactct aaaaaaaaat
     1261 gagctttttt ttctaaagcc ttcaagctta tttaccgaca ctaaatatgt gagggattct
     1321 atgcaaggga tacaagaaat tggcatcgct tcggttgcac agcctactgt gatctcctca
     1381 gaggatttga aggcgctttt gtacctgaca atccgcgggc acaacggcct caccggagct
     1441 gccgtagaag aaaaaaatag cgttcgcacc actgaagatc gtggcatcga ttttgaaaag
     1501 ggtgtagacg ttcgggcttg atacgcttat ccaagcgtag caagcggttt tactatggaa
     1561 agtatgacgg gttatggcga aagccatttc aacgccgagg gtatatcgat tgtgtgttcg
     1621 gcacgcagtt taaatagccg ctttcttgaa gtagaactgg attacccgtc tgagctgctt
     1681 tggtttaaag cagcgagtga agcgattatc caacgccgtt tctaacgggg gcgtgtagag
     1741 attgtttttc aaagtgcagg tgcgcttcct tcgaatatta attttaacac cgaccttatt
     1801 tcacaatacg aaaaattagt cgcacagcgc gttcataaaa aaaacttcaa tgtagatccg
     1861 cgcgattttt tacacattcc cggatttgta gagcgccgcg ttaaagattg gc
//
LOCUS       DAOMPD010000517         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23149_length_1912_cov_3.287022, whole genome shotgun sequence.
ACCESSION   DAOMPD010000517 DAOMPD010000000
VERSION     DAOMPD010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23149_length_1912_cov_3.287022"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PLY93_13595"
     CDS             <1..1022
                     /locus_tag="PLY93_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRP70557.1"
                     /translation="GFQHAERAALNSIASIDVSHATLYATLEPCCHHGRTLPCVDIIF
                     ERKIKRVVVGERDFAAQVQGRSLAILQEQGVDVSLFEPHVFEKEAWFTTQPFFFAQKN
                     KRPRVTLKWAETQDGYIAPREGVSGKISGSAAAFITAALRNYHKFTLATPGTTAIDAP
                     QLNVRFDTPLASLENSGLSNFFQELLYLQSSLAKITDDQHTSIKRAERGFILDEASVS
                     EKFLTRQENMGGRYTFFPFEKSDWKNNFKTSAEKVFTQIYSRGFNSVLVEAGPTFARL
                     LLSHGFVDAVVVYRSKNKTAQNLWGDKGRRVPFADFTSRFALLEYGDLGDDELFFYRR
                     KNHDR"
     gene            1012..1863
                     /locus_tag="PLY93_13600"
     CDS             1012..1863
                     /locus_tag="PLY93_13600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRP70558.1"
                     /translation="MTVDVVIPAYERKELLREAVESVLAQTYNNFTLFIVDDASPTPL
                     VHNWRISDSRLRFVRLIKNEGPGRARNAGVKEGNAPLIAFLDSDDLWHKDKLRAQVDF
                     FRKNTSAEWVHTHEVWFRNGKEVKQKKEYRKEGGQFLTRAFERCLISPSSVVMRRSFF
                     ESVGGFEPHFYCCEDYELWLRLLLRAPIGYIDEPLTIKRAGAWSQLSQTPVLDRLRVL
                     ALHRFYRQLRNESTKQEYILPLLQEAEKKCTILLKGALKRNNNTQVTQYQAWLTLFNT
                     RRTLLIR"
BASE COUNT          493 a          438 c          488 g          493 t
ORIGIN      
        1 cggggtttca acacgccgag cgcgctgcgc tcaatagtat tgcgtctatc gacgtgtcgc
       61 acgccacact ttacgcgacg cttgaaccgt gttgtcacca cggccgcacg ctgccctgtg
      121 tggatataat ttttgaacgt aaaatcaaac gtgtggtggt cggcgagcgt gattttgcgg
      181 cgcaggtaca gggccgctcg ctggcaatac tccaagaaca aggggtggat gtctcgcttt
      241 ttgaacctca tgtttttgag aaagaagcgt ggttcacgac acagcctttt ttttttgcac
      301 agaaaaataa aagaccgcgc gtgacgctta aatgggcgga aacccaagac ggctatatcg
      361 cccctcgtga aggcgtatcg ggaaaaatta gcggcagtgc ggcggcgttc atcacggcgg
      421 cgttgcgtaa ttaccataaa tttactctcg ccacaccggg cacaaccgcc atcgacgcgc
      481 cgcagctcaa cgtgcgtttt gatacaccgc ttgcttcatt ggaaaactcg gggttgagta
      541 atttttttca agagcttctt tatttgcagt cgtcgttggc aaagattacc gacgaccaac
      601 acaccagcat taaacgtgca gagcgtggat ttattttaga cgaagcgtct gtttcagaaa
      661 aatttttgac acggcaagaa aacatgggtg ggagatacac tttttttcct tttgaaaaaa
      721 gtgattggaa aaataatttt aagacaagcg cggaaaaagt ttttacacaa atctattcgc
      781 gaggcttcaa tagcgttttg gtggaagcgg gaccgacgtt tgcacggctt ttactttcgc
      841 atggttttgt cgatgctgtc gtcgtttacc gctccaaaaa taaaaccgcg caaaaccttt
      901 ggggtgataa agggcgtaga gtgccttttg ccgattttac aagccggttt gcgcttttag
      961 aatacggcga tttaggcgac gacgaacttt tcttctaccg cagaaaaaat catgaccgtt
     1021 gatgtggtaa tcccagcgta tgagcgcaaa gagcttttac gcgaagccgt cgaaagcgtg
     1081 cttgcgcaga cgtataacaa cttcacattg tttatcgtcg acgatgcctc ccccacgcct
     1141 cttgtgcaca actggagaat tagcgacagc cgcttgcgtt ttgtgcgctt aataaaaaac
     1201 gaagggccgg gtcgtgcgcg caatgcaggc gtgaaagaag gcaacgcccc gcttattgca
     1261 tttttagaca gcgacgacct ttggcataaa gataagttgc gtgcgcaggt ggattttttt
     1321 cgcaagaata cgagcgcaga atgggtgcat acgcatgaag tatggtttcg caatggcaaa
     1381 gaagtgaaac aaaaaaaaga ataccgtaaa gaaggcgggc aatttttaac gcgtgcgttt
     1441 gagcgttgcc tcatctcgcc gtcgtcagtg gtgatgcgcc gttctttttt tgaaagcgtg
     1501 ggcgggtttg aaccgcactt ttattgttgc gaggattatg aactgtggtt acggttattg
     1561 ctgcgtgctc caatcggata tatcgacgaa ccgctcacga ttaagcgggc gggtgcgtgg
     1621 agtcaacttt ctcagacacc cgtactggat aggctgcggg tgttagcgtt gcaccgtttt
     1681 tatcgccaac tacgtaatga atccacgaag caagaataca tcctgccgct tctccaagaa
     1741 gctgaaaaaa aatgtacaat acttttgaaa ggcgcgctaa agcgtaacaa caacacccaa
     1801 gtaacgcagt atcaagcttg gttgactttg tttaacaccc gccgtacgtt actcatacgt
     1861 tgatcgtcgc ggcctttttc taagtactgc ggtgcatatt caaccacaag cg
//
LOCUS       DAOMPD010000518         1910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23180_length_1910_cov_2.407547, whole genome shotgun sequence.
ACCESSION   DAOMPD010000518 DAOMPD010000000
VERSION     DAOMPD010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23180_length_1910_cov_2.407547"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLY93_13605"
     CDS             <1..536
                     /locus_tag="PLY93_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70559.1"
                     /translation="ASVEETTIEDYEWLMGINWWGVLYGTKFFLPHLQKVDEAHIVNI
                     SSVFGLMGVPTQSAYNAAKFAVRGFTECLRQELVDTHIGVSCVHPGGIKTNIAASARF
                     KTSPTGEKLDHSVAADKFAQIARTTPEEAAATIVRGIEKKSPRVLIGADAVAIDLMVR
                     ALPESYATVLGAALKLS"
     gene            592..1692
                     /gene="ispG"
                     /locus_tag="PLY93_13610"
                     /gene_synonym="gcpE"
     CDS             592..1692
                     /gene="ispG"
                     /locus_tag="PLY93_13610"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRP70560.1"
                     /translation="MAESTTHEHDTIPRRKTRTVMVGDVPVGSGHPVPVQSMTKIKTA
                     DWKGTVEQILRYEEAGCQIVRCTANNEEAAEALKKIKENIHIPLVADIHFRHKLALIA
                     LKSGVDKIRINPGNIGSKEKVAEVLEACKDRNVPIRIGVNAGSLEKDILKKVGYPTSD
                     GMVESALRHLEICDEHNFNNIIISLKSSDVYMMMEAYRKLAKMCDYPFHLGVTEAGTS
                     WQGTIKSSIGIGGLLAEGIGDTIRVSLTTDGVEEIKVGREILRSLGLVSFGVTLVSCP
                     TCGRLEVDLFRIAKEVEEAVAHIKTPLKVAVMGCLVNGPGESKGADIGISSGSGSAVL
                     YVKGESKGKIDESEIVPRLLEEIRILEESMKK"
     gene            complement(1700..>1910)
                     /locus_tag="PLY93_13615"
     CDS             complement(1700..>1910)
                     /locus_tag="PLY93_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70561.1"
                     /translation="IAVGARIPLYKNIALLASVNIQQAATTAISGNTPTATDVSGNTI
                     SYAPLTVLSTNIGVSLGIVRRFDIF"
BASE COUNT          489 a          451 c          544 g          426 t
ORIGIN      
        1 cggcgagtgt ggaagaaacc accattgaag attacgagtg gctcatgggg attaactggt
       61 ggggcgtgct ttatggcacc aagttttttt taccgcactt gcaaaaagtc gacgaagcgc
      121 acatcgttaa tatctcgagc gtgtttggat tgatgggcgt gccgacgcaa tcggcgtaca
      181 acgcggcgaa atttgccgta cgcggtttca ccgaatgctt gcgccaagag ttggtggata
      241 cccacattgg ggtaagctgc gttcatcccg gcggtattaa aaccaacatt gccgctagcg
      301 cacgtttcaa aacgagcccc acaggagaaa aactcgacca tagcgtcgcc gcagacaagt
      361 ttgcacaaat agcgcgtacc acacccgagg aagcagcggc gacgattgtg cgcgggattg
      421 aaaagaaaag cccccgtgtg ctcatcggcg ccgatgccgt ggcgatcgac ctcatggtgc
      481 gcgcgctccc cgagagctat gccacggttt taggcgcagc gttgaaattg agttagtttt
      541 ccagagtcgt atttctcttt tctgcctgtt tcactcctcg atgagggtga tatggctgag
      601 agcaccacac acgaacacga caccatcccc cgtcgcaaaa ctcgtacagt gatggtcggc
      661 gatgtgcccg tggggtcagg ccatcccgtg ccggtgcagt cgatgacgaa aattaaaacc
      721 gccgattgga aaggcacggt cgaacaaatc ttgcgctacg aagaggcggg gtgccagatc
      781 gtgcgctgta cagcgaacaa cgaagaagcc gccgaagcgc tcaagaaaat taaagaaaac
      841 atccatatcc cgttggtggc cgatatccac ttccggcaca agctcgcgct catcgctctc
      901 aaaagcggtg tcgataaaat ccgcatcaat ccggggaata tcggcagcaa agaaaaagtc
      961 gccgaggtgc tggaggcttg caaagataga aatgtcccca tccgcatcgg tgtgaatgcc
     1021 ggttcgttag aaaaagacat tctcaaaaaa gtggggtacc ctacatcgga cggcatggtg
     1081 gagtcggcgt tacggcattt agagatttgc gatgaacata attttaataa tattattatc
     1141 tctctcaagt cgagcgatgt ctacatgatg atggaagcgt accgtaagct cgcgaaaatg
     1201 tgcgactacc cattccactt gggagttacc gaggcgggca ccagttggca aggtaccatt
     1261 aaatcttcaa tcggcattgg cggccttctt gccgaaggga ttggcgacac aattcgggta
     1321 agcctcacca ccgatggcgt tgaagaaatt aaagtggggc gtgaaatttt gcgctcgttg
     1381 gggttggtga gttttggcgt gacgttggtg tcttgcccca cttgcggccg tctcgaagtg
     1441 gatttatttc gtatcgcgaa agaagtcgaa gaggcagtgg cgcatatcaa gacaccgctt
     1501 aaagtcgcgg tgatggggtg cctcgtcaac ggaccgggcg aatcgaaagg cgccgacatc
     1561 ggcatttcgt cggggtctgg ctctgcagtg ctttatgtta aaggggaaag taaaggaaag
     1621 attgacgagt cagaaattgt gccgcgtctt ttagaagaaa ttcgtatcct cgaagaatcg
     1681 atgaagaaat aatcgcaaac taaaaaatat caaatcgccg cacgatgccc agcgaaacgc
     1741 cgatattcgt cgacaaaacg gtgagaggtg cgtagctaat agtgtttccc gaaacgtcgg
     1801 tggcggtggg tgtgtttccc gagatggcgg ttgtggcggc ttgttgtata ttcacactgg
     1861 ccaaaagtgc gatgttcttg tataaaggga tgcgcgcccc tactgcgatg
//
LOCUS       DAOMPD010000519         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23224_length_1907_cov_1.648488, whole genome shotgun sequence.
ACCESSION   DAOMPD010000519 DAOMPD010000000
VERSION     DAOMPD010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23224_length_1907_cov_1.648488"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLY93_13620"
     CDS             complement(<1..201)
                     /locus_tag="PLY93_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70562.1"
                     /translation="MFGAGCDGFAFGPVLSNDNNGPVYGVSAGYTYFLIDRLGASLNA
                     SAIFGSDYQQYGVGPAFTYFVGP"
     gene            complement(389..1063)
                     /locus_tag="PLY93_13625"
     CDS             complement(389..1063)
                     /locus_tag="PLY93_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRP70563.1"
                     /translation="MSIPVNFLTAYHALFETGTLLKNAQVLVHAGAGGVGLAAIQLAK
                     NHGCTVFATAGTPKKIELLKEYGVDYPINYRETDFEREVLRLTQGEKIDVVLDSVGGS
                     YFKKDLNLVRAHGRVVAYGAAAFTDRSLFKLPTILPQLLSMLTLSMVDLMTNSRGFYG
                     VNMLRVAEECPDLLQRELQEIMQLFAAEELTTIVSQQLSWREAHKAHEVLEGRLSTGK
                     IVLVVD"
     gene            complement(1047..1403)
                     /locus_tag="PLY93_13630"
     CDS             complement(1047..1403)
                     /locus_tag="PLY93_13630"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRP70564.1"
                     /translation="MKAIPFQRASDASSLRLENWPEPEAKTHEILIQVRAFGVNFADI
                     LARKGEYGDAPHFPFIPGYEVAGVVNQLGSNVKKFKVGDAVLALTHFGGYAEYAVADE
                     RAAVLKPKNLNFVHPR"
     gene            complement(1411..>1907)
                     /locus_tag="PLY93_13635"
     CDS             complement(1411..>1907)
                     /locus_tag="PLY93_13635"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HRP70565.1"
                     /translation="AGLKDKLTKMNKDDIAPLFWYAFAWGSAINIDREDTEALSNLPK
                     IEVMMAQVKEWDASFYFAGAWLFEGVYYGARSEMMGGNPTRSKEAFENNLKLTGRKLL
                     MTKYFYARTYCIFAQDKACFEKNLKEVLDTPLDIFPEQKLANVLAKKKAERLLKRSEE
                     FFVE"
BASE COUNT          491 a          483 c          429 g          504 t
ORIGIN      
        1 agggcctaca aaataggtga acgccggtcc gacaccgtat tgttgatagt ctgaaccaaa
       61 aattgccgat gcgtttaaag aagcccccaa acgatcaatc aggaaatacg tgtagcctgc
      121 gctcactcca tacaccggcc cattattatc gtttgaaagg acggggccaa aagcaaaacc
      181 atcgcaccct gcgccaaaca cgaactcttg gcaacgcgtc gttcggtggg agtgactttg
      241 tgttgcgcta cgtgattcgt cgatacacga ttatcttcta cagccaagac gttagctatt
      301 gcagaaaaaa agaataaact cattattaca aaagaatatc gattgagctt catcattttc
      361 tctttgtttt taacaacctt ttcaaaattt aatccaccac taagacaatt tttcctgtgc
      421 tcaatcggcc ttcgagaact tcatgcgctt tgtgtgcttc gcgccacgag agctgttgcg
      481 atacaatcgt tgttaattct tcagcggcga aaagctgcat tatctcttgc aattcgcgct
      541 gcaataaatc agggcattcc tctgccacgc ggagcatatt gacgccatag aaaccacggc
      601 tattggtcat taaatcgacc atcgagagag tgagcatgct taaaagttgc ggcagaattg
      661 tgggcagctt aaataaacta cgatcggtaa atgccgccgc accgtacgct accacgcggc
      721 catgcgcgcg caccagattt aaatctttct tgaaatacga accgcccacc gaatctaaaa
      781 ccacatcaat tttttccccc tgcgtgaggc gtaatacctc gcgttcaaaa tctgtttcgc
      841 ggtagttaat gggataatcc acgccatatt ctttgagcag ttcgattttt tttggggttc
      901 ccgccgtcgc aaacacggta cagccgtggt tcttcgccaa ctggattgcc gctagcccca
      961 ccccacctgc gcccgcgtgg acaagtactt gcgcgttttt aaggagggta ccggtttcaa
     1021 aaagcgcgtg gtacgctgtt aagaaattaa cggggatgga caaagttcaa attcttcggt
     1081 ttcaaaaccg ccgcgcgttc gtcagcaacg gcgtactcgg cgtaaccgcc gaaatgagtg
     1141 agcgcgagga cagcatcgcc cactttaaat tttttgacat tactccccag ttggttcaca
     1201 acaccagcga cctcgtatcc tggaataaac gggaaatggg gcgcatctcc gtactcacct
     1261 ttacgcgcga ggatgtcggc aaagttgacg ccaaacgcgc gcacttggat taaaatttca
     1321 tgcgttttcg cttcaggttc tggccaattt tcaaggcgta gcgacgaggc gtccgatgcg
     1381 cgttggaatg ggattgcttt cataattcct ctattcgaca aaaaattctt cgctacgttt
     1441 caaaagacgt tctgcctttt tcttcgcgag cacgttggcg agcttttgtt cggggaaaat
     1501 atcgaggggt gtatccaaaa cctctttgag atttttttca aagcacgcct tgtcttgcgc
     1561 aaaaatacaa tacgtgcgcg cgtaaaagta cttcgtcatg agaagctttc taccggtgag
     1621 ttttaaatta ttttcaaacg cttctttaga acgggtggga tttcccccca tcatttcgga
     1681 gcgcgctccg taataaacgc cttcaaagag ccatgcaccg gcgaagtaaa aagaggcatc
     1741 ccactctttg acttgggcca tcatcacttc aattttaggc agattcgaga gcgcctcggt
     1801 gtcttcgcga tcgatgttga tggcagagcc ccacgcgaat gcataccaaa aaagaggcgc
     1861 gatgtcgtct ttattcattt tcgtgagctt gtcttttaat ccagcct
//
LOCUS       DAOMPD010000520         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23277_length_1904_cov_1.514332, whole genome shotgun sequence.
ACCESSION   DAOMPD010000520 DAOMPD010000000
VERSION     DAOMPD010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23277_length_1904_cov_1.514332"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLY93_13640"
     CDS             <1..325
                     /locus_tag="PLY93_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70566.1"
                     /translation="LVNIALENNFLIKTKIKFYIFLANPKQKKDPKASKGYVHESDVI
                     AMAKAAGFKLIAKSEINANKKDTKDYPEGVWTLPPSYRLGDKDKTKYAAIGESDRMTL
                     KFQKP"
     gene            complement(397..>1646)
                     /locus_tag="PLY93_13645"
     CDS             complement(397..>1646)
                     /locus_tag="PLY93_13645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728565.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRP70567.1"
                     /translation="FDINYVFNRFLIGEAFLINTLKIPKEVLNKSDFNLLQHLGFTAE
                     QIQIANEVICGTMMIEGAPYLKGEHLSVFDCANKCGRKGQRYIHYNAHLNVMAAAQPF
                     ISGAISKTINMPYEATVEDVKNAYMASWKLMIKANALYRDGSKLSQPLSSVGENIFDA
                     LEDDTLPEDMAADIAMSPTALKAEKMAEKIIEKIIVQRVAERRRLPSRRQGYTQKAVI
                     GGHKVYLRTGEYEDGSLGEIILDMHKEGAAFRSLMNGFAIAVSLGLQYGVPLEEFVEA
                     FVFTKFEPNGVVQGHDRVKMATSVMDFIFRELAITYLARNDLAHVSPDLAFDTPAKLN
                     DARTAEKVKPKTEVKAQPQPVLVGASTSRAGLSVKVTASQIQEARLKGYEGTSCPECS
                     SMTLVRNGSCLKCETCGATTGCS"
     assembly_gap    1647..1656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1657..>1904)
                     /locus_tag="PLY93_13650"
     CDS             complement(<1657..>1904)
                     /locus_tag="PLY93_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70568.1"
                     /translation="IEPDFALVKFKKLAGGGYFKIVNKSVPLALEHLGYSKDQIAKIV
                     EYMVGTATFTGAPHINRENLTLRGFTAEVLDTIEEALP"
BASE COUNT          495 a          462 c          444 g          493 t
ORIGIN      
        1 gttggttaat atagctttag aaaataattt tttgataaaa acgaaaatta aattttatat
       61 atttttggca aatccaaaac agaaaaaaga ccctaaagca tcgaagggat atgtccacga
      121 aagcgacgtg attgctatgg caaaggcagc agggtttaag ctcattgcca aatcagaaat
      181 caatgcaaac aagaaagaca ccaaagatta tcccgagggt gtatggacac tgccaccgag
      241 ttatcgctta ggcgacaaag ataaaacaaa gtacgccgct atcggcgaga gcgaccgcat
      301 gacgctcaaa tttcaaaaac cgtaatgata ttttggcagg atagccattc catcccgcct
      361 ctatgcagcg tgcttaaata ccactaacgc ggaggattac gaacaccccg tcgtcgcgcc
      421 gcatgtttcg cacttgaggc acgaaccgtt gcgtaccaac gtcattgaac tgcactcggg
      481 gcatgaagtg ccttcgtagc ctttcaggcg cgcttcttga atttggcttg ccgtcacttt
      541 cacactaagc ccagcacggc ttgtcgatgc gccgacgaga accggttgcg gttgtgcttt
      601 cacttccgtc ttgggtttta ctttttcagc ggtgcgagca tcgtttagct ttgctggcgt
      661 gtcgaacgcc aaatcggggg aaacgtgtgc caagtcattg cgcgcaagat aggtaatcgc
      721 gagttctctg aaaataaaat ccatcaccga ggtggccatt ttcacacgat cgtgcccctg
      781 caccacgccg ttgggttcaa atttagtgaa cacaaacgct tcgacaaact cttcgagcgg
      841 cacaccgtat tggagaccca acgaaacggc gatggcaaaa ccgttcataa gcgagcggaa
      901 cgccgcgcct tctttgtgca tatcaagaat aatttcaccg agtgaaccgt cttcgtattc
      961 gcccgtgcgc aaataaactt tgtggccacc gatcactgcc ttttgggtat agccttggcg
     1021 gcgggacggt agacggcggc gttcggcaac gcgttgcacg ataattttct caataatttt
     1081 ttctgccatc ttttcggctt taagcgcagt gggagacatt gcgatgtcgg cagccatgtc
     1141 ttctggaaga gtatcgtctt cgagtgcgtc aaaaatattt tcacccacgc tcgacaaagg
     1201 ttgcgacagt tttgaaccat ctcggtaaag cgcgttggct ttaatcatga gcttccacga
     1261 cgccatgtat gcattcttca cgtcttcgac ggtcgcctcg tatggcatgt tgatcgtttt
     1321 tgaaatcgcg ccgctgataa aaggctgcgc cgccgccatc acgtttaagt gcgcgttata
     1381 gtggatatag cgctggcctt tccgtccgca tttattcgca caatcaaaaa ccgaaaggtg
     1441 ttcgccttta agatacggtg ccccctcaat catcatggtg ccacaaatga cctcgttcgc
     1501 tatctggatt tgctccgcag taaatcccaa atgctgtaat aaattaaaat cggatttgtt
     1561 caaaacttct ttggggattt tcagcgtgtt gatgaggaac gcctcgccga taagaaagcg
     1621 attgaataca tagttaatat caaacgnnnn nnnnnngggg agagcctctt caatggtatc
     1681 taaaacctct gcggtgaaac ctctgagagt caaattttca cggttgatat gcggcgcacc
     1741 ggtgaaggtg gcagtcccca ccatgtactc gacaattttt gcaatttggt ctttgctata
     1801 gcccaagtgt tccaacgcga gcggtaccga tttattcaca attttaaaat aaccgccacc
     1861 tgcgagtttt ttgaatttta cgagcgcaaa gtcaggctcg atgc
//
LOCUS       DAOMPD010000521         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23392_length_1895_cov_2.729891, whole genome shotgun sequence.
ACCESSION   DAOMPD010000521 DAOMPD010000000
VERSION     DAOMPD010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23392_length_1895_cov_2.729891"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>953)
                     /locus_tag="PLY93_13655"
     CDS             complement(<1..>953)
                     /locus_tag="PLY93_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70569.1"
                     /translation="TTYTPPTPTLTDGGFYNNNDPTDKRPRLLQAMLNVNDGEAFSYI
                     TGDVQDTSKTINMMNGTRRLNSLTQIVNMMPGQITATLINNTDPAAVTRSLVYMTNNL
                     DDDETGTAKAFASMIHYGTKIVAQGRELEPYDCNCTWTGCDTCLREYVPATPTCLEFT
                     AIGPRRMAAVLNLEAGPYLEALLSGLGWQISIAAMNCGWARDDGDNSGAVQCTSFPDR
                     DNASGGMLKDVGDSYNGVPKPTARQCIPAREITAGYKYGNYTWGASNEQGIRWQNCQS
                     IMPWKSGGSFIATAEGDTDNIIWNGLSAPLGLGTIEKGIGQ"
     assembly_gap    954..963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<964..1881)
                     /locus_tag="PLY93_13660"
     CDS             complement(<964..1881)
                     /locus_tag="PLY93_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70570.1"
                     /translation="MNTLVDTHGNETYGVGALGSYNGAVLGWYGNTPSGTGCTTTDCD
                     TNTTDKLGRLIQMINNIEQIPNTVYFMVYVQDIGYASYLIDNLTRIRYMTDIVNNLTN
                     VDLMVKVMNGADACSVYSGVNNTVATDTSGNCTTAGGTWFGVGRCKNFPTQTTRAACL
                     AAGGGTGIWEGADRAKMLALLNGLGTSTKRGTFTKNVGDMYTLSDVVNRLGYDGSIAR
                     SSGQQVRVVRLMNGVENCGLKAQYDKYIQYQPTCVDGSGNPRPLNFQFKEACQAAGAG
                     YNWQTKASNTAWVSPSTIYYDCGTVAADGT"
BASE COUNT          436 a          471 c          457 g          521 t
ORIGIN      
        1 ttgaccaatc cctttttcaa tagtacctaa acccaatggt gcagataaac cattccatat
       61 aatattatca gtatcaccct cggcagttgc tataaaagaa ccgcctgatt tccaaggcat
      121 gatactctgg cagttttgcc aacgtatccc ttgctcattc gatgcacccc aagtataatt
      181 gccatattta taacctgctg ttatttcgcg agcaggtata cattgtcgtg cagttggttt
      241 tggtacacca ttgtagctat cgcctacatc cttgagcata cctcctgatg cattgtctct
      301 atcgggaaat gacgtgcact gtactgcacc agaattatcg ccatcgtctc gtgcccaacc
      361 acagttcatc gctgcaatcg agatctgcca acccaagccc gagagcaaag cttcaaggta
      421 tggccctgct tcaaggttta agacggctgc catccgccgt ggaccaattg ctgtgaattc
      481 aaggcaggtt ggtgtagcgg gtacatattc tctcaaacag gtgtcgcaac ccgtccaggt
      541 acaattacaa tcgtacggct ctaactcacg accttgtgct acaatttttg taccgtagtg
      601 gatcattgat gcaaatgctt ttgcagtacc ggtttcgtcg tcgtcgaggt tatttgtcat
      661 ataaacgagt gaacgagtaa ccgccgcagg gtcagtattg ttgatgaggg tagcggtaat
      721 ttggcctggc atcatattca caatttgagt gagcgagttt aaccggcggg taccattcat
      781 catattgata gttttagatg tatcttgcac gtcacctgta atatacgaga acgcctcacc
      841 gtcgttgacg ttgagcatcg cttgcaaaag gcgggggcgc ttatcggtgg gatcgttgtt
      901 gttgtaaaaa ccaccatccg tcaatgtggg tgtggggggg gtgtacgttg ttgnnnnnnn
      961 nnncgtaccg tcggctgcga cagttccaca atcataataa attgtagagg gtgataccca
     1021 agccgtatta gatgcttttg tctgccaatt ataccccgcg cctgctgctt ggcacgcttc
     1081 tttgaactga aaattgagcg gacggggatt tccactgccg tctacgcagg tgggttggta
     1141 ttgaatatat ttatcgtatt gggcttttag accacagttt tcaacgccgt tcatgagccg
     1201 tacaacacgc acttgttgac cgctggaacg agcgattgaa ccatcatatc ccaaccgatt
     1261 caccacatcg ctgagggtgt acatgtcgcc cacgttcttt gtgaatgtac cgcgttttgt
     1321 cgatgtacct aagccattga ggagtgcgag catctttgcc cggtctgccc cttcccatat
     1381 accggtacct ccccccgccg caagacacgc agcacgggtg gtttgtgttg gaaaattttt
     1441 acacctaccc acgccaaacc aagtaccacc ggcggttgta cagtttccac tcgtgtctgt
     1501 agcgaccgta ttgtttactc ccgaatacac cgagcacgca tcggcgccgt tcattacttt
     1561 gaccatgagg tcaacgttgg tgaggttgtt cacgatgtcg gtcatgtagc ggatacgagt
     1621 caggttatcg atgaggtacg aggcgtagcc aatatcctgg acgtagacca tgaaataaac
     1681 cgtgttgggt atttgctcga tattattaat catctggatt aggcgtccca gtttatcggt
     1741 cgtgttcgtg tcgcagtcag ttgtcgtaca acccgttcca ctgggggtgt taccgtacca
     1801 accgaggaca gcgccattgt aggagcccaa agcgcccacg ccatacgtct cgttaccgtg
     1861 tgtgtctaca agcgtgttca cgcgcgaagg ttgga
//
LOCUS       DAOMPD010000522         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23434_length_1892_cov_2.483941, whole genome shotgun sequence.
ACCESSION   DAOMPD010000522 DAOMPD010000000
VERSION     DAOMPD010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23434_length_1892_cov_2.483941"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PLY93_13665"
     CDS             <1..1228
                     /locus_tag="PLY93_13665"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRP70571.1"
                     /translation="EEGVPLKASVLVGGPPALILAAVMPLPETISEILFASMLGKRSF
                     KYKRRGGYLLSPFVDFAIHGEISNQMLPEGPFGDHLGYYSLKHDFPHLKVDKILVRKK
                     NAIWPFTVVGRPPQEDSEFGALIHSLTKDLISHEVPGVREVNAVDAAGVHPLLLAVGS
                     ERYLPYAEKKPREILTQANAILGFNQMSLAKYLFITAEEGSVKLSTHRVEDFLVHILE
                     RIDLTRDLHFQTCTTMDSLDYSGHALHEGSKLIVAAYGEKRRTLATVLPERAMKRYTF
                     RDARLALAGVIVVRTTAFKDYKSTEYEMRSLGSSCREFLEEGIVLIVVADDAEYTAKN
                     LDNFLWTTFTRSDPARDIYGVDSFTENKHWGAKGAIIIDARVKHHHAGMLVEDAAVSE
                     NINRFFKKGASLEKFH"
     gene            1288..1461
                     /locus_tag="PLY93_13670"
     CDS             1288..1461
                     /locus_tag="PLY93_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70572.1"
                     /translation="MAKEIIQNKIQSGEKVKVLDLEKYRLKKKLLDAGVQYYENKAGK
                     FTLWVRTAQLTHY"
     gene            1464..>1892
                     /locus_tag="PLY93_13675"
     CDS             1464..>1892
                     /locus_tag="PLY93_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRP70573.1"
                     /translation="MKLLSFLTRKTSSHKTYKNPVPTVDIIIEIQSGIVLIERKNPPY
                     GWAIPGGFVDYGECLEDAARREAKEETSLVVTLKNLLYVYSNPERDPRQHTVSTVFIA
                     TAKGTPIAADDAKTLKVFPLNKIPKDMAFDHAQILKDYIRF"
BASE COUNT          533 a          452 c          431 g          476 t
ORIGIN      
        1 agaagagggc gtaccgctta aggccagcgt gcttgtgggt gggccacccg cgctcatttt
       61 agctgctgtg atgccgcttc ctgaaactat cagcgaaatt ttgttcgcgt cgatgttggg
      121 caaacgctct ttcaagtata aaaggcgcgg cggttatctc ctttctccct ttgttgactt
      181 tgccatccac ggcgaaattt caaaccaaat gctgcccgaa ggaccattcg gcgaccactt
      241 gggctattat tcgctcaaac acgattttcc tcatttgaaa gtcgataaaa ttttagtacg
      301 taaaaaaaat gcaatttggc ctttcaccgt cgtcggacgc ccaccgcagg aagattcgga
      361 gttcggcgcg ctcatccatt cgctcactaa agatttaatt agccacgaag tgcccggtgt
      421 acgcgaggtc aacgcggtcg atgccgccgg tgtacatccc ttactcttag cggtggggag
      481 cgagcgctat ctaccgtatg cggaaaaaaa gccgcgtgag attttaacgc aggccaacgc
      541 aatacttggc tttaatcaaa tgtcgctcgc aaagtatcta tttattacgg cagaagaggg
      601 cagtgtgaag ctatcgacgc accgtgtgga agatttctta gtgcatattt tagagcgcat
      661 cgatttaacg cgcgatcttc attttcaaac atgtaccacg atggattcgc tcgattattc
      721 ggggcacgct ctccacgaag gatcaaagct tatcgtcgcg gcgtacggtg aaaagagacg
      781 cacgcttgcg accgtgctgc ccgaacgtgc catgaaacgc tacaccttcc gcgatgcccg
      841 gttggcattg gcaggcgtta ttgttgtacg cacgaccgca ttcaaagatt ataaatcaac
      901 cgaatacgaa atgcgctctt tgggaagtag ttgccgtgag tttttagaag aagggattgt
      961 actcatcgtc gttgccgacg acgccgagta caccgcaaaa aatctcgata attttctttg
     1021 gacgaccttc acgcgttctg accctgcacg cgacatctat ggcgtcgata gctttacgga
     1081 aaataaacat tggggagcaa aaggggcaat cattatcgat gcgcgggtaa aacaccacca
     1141 tgcaggaatg ctcgtcgaag acgccgcagt gagcgaaaat attaaccgct ttttcaagaa
     1201 aggcgcatcg cttgaaaaat ttcattaagc gacataacat tttcccaaac tttcaatttt
     1261 gcactatgta gaatccgata cttaaatgtg gctaaagaaa tcatccaaaa taaaattcaa
     1321 tctggcgaaa aggtaaaagt tttagacctt gaaaaatatc gtctaaagaa aaaactcctt
     1381 gatgcgggtg tgcaatacta cgaaaacaaa gcggggaagt ttactctgtg ggtgcgaact
     1441 gcgcagttga cgcattactg aaaatgaaat tactttcttt ccttactcga aaaacaagct
     1501 cccacaaaac atacaaaaat cctgtcccca ccgttgatat catcattgaa atccaaagtg
     1561 ggattgtact cattgaacgt aaaaatcccc cttacggttg ggcaatcccc ggcggttttg
     1621 tcgattacgg tgaatgctta gaagatgccg cgcggcgtga agccaaagaa gagacttcgt
     1681 tagttgtcac gctgaaaaat cttctttacg tgtactcaaa tccagaacgg gatccaaggc
     1741 agcacaccgt ttcgactgtt tttatcgcca ctgccaaagg aacgcctatt gccgccgacg
     1801 atgccaagac tctcaaagta tttcctctga acaaaatacc gaaagatatg gcatttgacc
     1861 acgcgcaaat cttaaaagat tatatccgtt tc
//
LOCUS       DAOMPD010000523         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23518_length_1885_cov_2.654645, whole genome shotgun sequence.
ACCESSION   DAOMPD010000523 DAOMPD010000000
VERSION     DAOMPD010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23518_length_1885_cov_2.654645"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLY93_13680"
     CDS             complement(<1..338)
                     /locus_tag="PLY93_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70574.1"
                     /translation="MKKILVTSFLLTGLSPLMAASSVEFLQNGFGAKSAGLGNAYGVV
                     VQDPSALYWNPAGLAQIYSEKRITQTVDAVRDGKALADDEEFNKLLEDQNTSGTPDSK
                     TVEITERSTQV"
     gene            complement(349..720)
                     /locus_tag="PLY93_13685"
     CDS             complement(349..720)
                     /locus_tag="PLY93_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70575.1"
                     /translation="MKKIRYSAFLLLAFTACMNMPADEASFKAYPNPYDPSKGVLTIE
                     KLSGTAYTLGNQHDLIVYDYSLTETYRAQVTPNGSNQLVWAGIDEGGTRVAPGVYFLK
                     VIVTTATGVENGNEMLKLIVQ"
     gene            complement(793..1149)
                     /locus_tag="PLY93_13690"
     CDS             complement(793..1149)
                     /locus_tag="PLY93_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70576.1"
                     /translation="MPEKLLYDAKEPLFINNHRPVSDAFLNLFYGTINLAQIHRFEKL
                     YRSLKTEIETINMGPQQFIAQLNGAFNRYQEHRRHDAFEAMDDKALKYVLEFMEKSIR
                     DVAKEVQKNTAKSLIG"
     gene            complement(1209..>1885)
                     /locus_tag="PLY93_13695"
     CDS             complement(1209..>1885)
                     /locus_tag="PLY93_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP70577.1"
                     /translation="ADDLIAQKFDAVILATGIIPRKLNIPGAENANVLSYIDVLRDRK
                     KVGKNVAVVGGGGIGFDIAVYLTDPGFDTWKDPKEFAKEWGFDLQVGTRAGLTQKAAD
                     HIASPRKVTMFKRSKGKFGGSLGKTTGWVHKLTLEERGVDQISSVTYEKIDEQGLHYV
                     LKGETKVLPVDTIVVCAGQEPKRDLEAGLVAAHKAVHIIGGASEAGELDAKRAIAEGA
                     QLGARL"
BASE COUNT          421 a          457 c          446 g          561 t
ORIGIN      
        1 acttgggtgc tgcgctcggt aatctctacg gttttagagt cgggcgtgcc gctggtgttt
       61 tggtcttcca agagtttatt aaactcttcg tcgtcggcta atgcttttcc atcacgcacg
      121 gcatccacag tctgcgtgat acgcttttca gagtaaattt gtgcaagacc ggcggggttc
      181 cagtagagcg ccgacgggtc ttgcacgaca acgccgtacg cgttgccgag ccccgcgctt
      241 ttggcgccga aaccgttctg caaaaattca accgacgaag cggccattaa gggggataga
      301 cctgttagaa gaaaagaagt cactagtatt tttttcataa ccgtttcctt attgtacgat
      361 gagtttcagc atttcgttac cgttttcaac gcccgtggcg gtggtgacga tgactttgag
      421 aaaatacacg ccgggcgcga cacgggtgcc gccctcgtcg atgcccgccc ataccagttg
      481 atttgaacca ttgggtgtga cctgtgcgcg gtaagtttcg gtgagactgt agtcgtacac
      541 aatgagatcg tgttgattac cgagagtata ggctgtgccg ctgagttttt caatcgtcag
      601 tacgcctttc gacgggtcgt acggattggg gtatgcctta aacgaggctt cgtcggcggg
      661 catgttcatg cacgcggtga acgccagtaa taaaaaagct gagtagcgta ttttcttcat
      721 ttttacctct tttttaaata actatgcctg tttttcaata actaaggatt tttcaataat
      781 catgtatgtt ttttatccaa tgagactttt ggcggtattt ttttgcactt ctttagcgac
      841 atcccgaatc gacttttcca taaactcgag tacatacttg agcgctttgt cgtccatcgc
      901 ttcaaacgca tcatgccgcc ggtgttcttg gtagcggtta aaagcgccat tgagctgtgc
      961 gataaattgc tgtggaccca tgttaatggt ttctatctcg gtttttaacg aacgataaag
     1021 tttttcaaac cggtgtattt gtgccaagtt gattgtgccg taaaataaat tcaaaaaagc
     1081 atccgatacg ggacggtggt tatttatgaa gaggggttct tttgcatcgt agagtaattt
     1141 ttctggcatc gttagttatt ccaatatgta tttggacgta attttttctt tattatcgac
     1201 aaacacgcct ataatcttgc ccccaactgt gcaccttcgg caatcgcccg cttcgcgtcg
     1261 agttcgcccg cttcactcgc gccaccgatg atgtgcaccg ctttgtgtgc cgccacaaga
     1321 cccgcttcta aatcgcgttt gggctcttgg cctgcgcaca caacaatcgt gtcgacgggc
     1381 aatactttgg tttctccttt taatacatag tgcaaacctt gttcgtcaat tttctcgtat
     1441 gtgacgcttg aaatttgatc cacaccccgc tcttctaagg tgagcttatg cacccaacct
     1501 gtggttttgc cgagtgaacc gccaaactta cctttgctgc gtttgaacat cgtcacttta
     1561 cggggcgatg caatgtggtc ggcggctttt tgagtgagcc ctgcgcgggt gcccacttgc
     1621 aaatcaaagc cccattcttt ggcaaactct ttggggtctt tccacgtatc aaaccccggg
     1681 tcggttaaat agacggcgat gtcaaaacca atgccgccac cgcccaccac ggcgacgttc
     1741 ttgccaactt ttttacgatc gcgcaaaaca tcgatgtagc tcaaaacatt tgcattttca
     1801 gcgccgggga tgttgagctt acgtggaata atccctgtag ctaaaataac cgcgtcaaat
     1861 ttctgtgcga ttaaatcgtc ggcag
//
LOCUS       DAOMPD010000524         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23558_length_1882_cov_2.402846, whole genome shotgun sequence.
ACCESSION   DAOMPD010000524 DAOMPD010000000
VERSION     DAOMPD010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23558_length_1882_cov_2.402846"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLY93_13700"
     CDS             complement(<1..672)
                     /locus_tag="PLY93_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRP70578.1"
                     /translation="MPAENRRVILSVKNVEKKFAHVHAVKNLSLKIFEGESVALLGPN
                     GAGKTTLLEMIEGLQKPDSGEILIDGLTYDKDEKKIRQLMGLAFQETRFPEKIKVGEA
                     LSLFAAFYGGSRARIKEILSLISLTEKKDAYVNNLSGGQRQRLALGIGIIQKPKIILL
                     DEPSTGLDPHARREIWHIMADLKKQGSTLLLTTHYMEEVERLCERIIILFKGKDLADG
                     PLSHLL"
     gene            complement(676..1689)
                     /gene="rfaE1"
                     /locus_tag="PLY93_13705"
     CDS             complement(676..1689)
                     /gene="rfaE1"
                     /locus_tag="PLY93_13705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRP70579.1"
                     /translation="MPEKKHLEAVLRKLPSSRIFVLGDLMWDEYVRGSVARISPEAPV
                     PVLLAQAEDRTPGGAANVLKNLRDLHCTMGILGIVGADANGMQLQHELEKWGIGDNLH
                     LIHLTDRPTTIKTRFMARNQQLLRVDRENASTIDAKIEDTILKVLERLIGGFNALIIS
                     DYDKGLLTKRVITEAIRIAKAKNVFVAVDPQVKHFKDYVGADIMTPNEKEASEGIGES
                     FPQNETEVEAIAKKIRSELKLGHLLITRSQRGMAYFNDSQSVYLPTVAREVFDVTGAG
                     DTVISVYTAAVAAGATNSEAMILSNTAGGIVVGKLGTATASVDEIRAALNHTLPELRV
                     VQY"
     gene            complement(1682..>1882)
                     /locus_tag="PLY93_13710"
     CDS             complement(1682..>1882)
                     /locus_tag="PLY93_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HRP70580.1"
                     /translation="GAPPHGGLAFGVDRVLMLALKRASIRDVIPFPKTQKGTCLMSGA
                     PSAVDMKQLRELYLKSTVVPHA"
BASE COUNT          448 a          478 c          460 g          496 t
ORIGIN      
        1 gagtaaatgc gacaaaggac catcggccaa atctttgccc ttgaagagaa taataatccg
       61 ctcgcataac ctttcgactt cttccatgta atgtgtggtt aaaagcaacg tcgagccttg
      121 ttttttcaga tcagccatga tatgccaaat ttcacgacgt gcgtgtgggt cgaggcccgt
      181 cgacggttcg tctaaaagaa taattttggg tttttgaatg atgccgatgc cgagcgcgag
      241 gcgctgccgt tgcccgcctg aaaggttatt tacatacgcg tctttttttt cggtgagcga
      301 aatgagcgac aaaatctctt tgatgcgcgc gcgactgccg ccataaaacg cggcaaaaag
      361 agacaacgcc tcgcccactt taattttttc ggggaaacga gtttcttgga atgccaatcc
      421 catgagctgt cgaatttttt tttcgtcctt gtcgtacgtt aggccgtcga ttaagatttc
      481 tcccgaatcg ggtttttgta gaccttcaat catctcaagg agagtcgttt tacccgcgcc
      541 gttgggaccc aaaagagcca ccgactcgcc ttcaaaaatt ttaagcgaaa gattttttac
      601 ggcatgcaca tgcgcaaact ttttctctac attttttacg gataaaataa cgcggcggtt
      661 ttcagcgggc attcgttaat attgcacgac acgtaattcg ggcagagtgt gattaagggc
      721 tgcacgaatt tcatcaacag aagcggtagc agttcccaat ttacccacga cgataccgcc
      781 ggcggtattc gacaaaatca tcgcttcgct attcgttgct cccgcagcga cagcagccgt
      841 ataaacgctg atcacggtat cgcctgcgcc ggtgacgtca aaaacttcac gcgccaccgt
      901 cggtagatac acgctttggc tgtcgttgaa gtacgccatg ccgcgctgcg aacgagtgat
      961 gagcagatga ccgagcttaa gttcactgcg gattttctta gcaatcgctt ccacttcagt
     1021 ttcattttga gggaagcttt cgccaatccc ttcggacgct tctttctcgt tgggggtcat
     1081 aatgtcggcg ccgacataat ctttaaaatg tttcacctgc ggatcgactg ccacaaaaac
     1141 attttttgct tttgcgatac ggatagcctc ggtgatgacg cgtttggtga gtaaaccttt
     1201 atcgtaatcg ctgataatga gcgcgttaaa accgccgatg aggcgttcaa ggactttaag
     1261 aatggtgtct tcgattttag cgtcaatcgt actggcattt tctctgtcga cgcgcaaaag
     1321 ctgttggtta cgcgccataa accgcgtttt gatcgtcgtg ggtctatcgg tcaaatgaat
     1381 gaggtgcaaa ttatcgccga taccccactt ttctaattcg tgttgcaact gcatgccgtt
     1441 cgcgtctgca cccacaatgc ccaagatgcc catcgtgcaa tgtaggtcgc gtaaattttt
     1501 gagcacgttt gccgcaccgc ccggcgttct gtcttctgcc tgtgctaaaa ggaccggcac
     1561 aggcgcctcg ggtgaaatac gcgcaacaga gccgcgcacg tattcatccc acatgagatc
     1621 gcccaataca aaaatgcgac tcgaaggtaa ttttcgtaaa accgcttcga ggtgtttttt
     1681 ttcaggcatg cggcaccacg gttgatttca aatagagctc gcgcaactgt ttcatgtcca
     1741 ccgccgaagg agcgcccgac ataaggcaag tgcctttttg agttttaggg aaaggaatca
     1801 catcgcggat cgacgcacgt ttgagcgcca gcatgagcac gcggtcgacg ccgaacgcca
     1861 agccaccgtg cgggggcgcg cc
//
LOCUS       DAOMPD010000525         1879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23600_length_1879_cov_1.746162, whole genome shotgun sequence.
ACCESSION   DAOMPD010000525 DAOMPD010000000
VERSION     DAOMPD010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23600_length_1879_cov_1.746162"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..487
                     /locus_tag="PLY93_13715"
     CDS             107..487
                     /locus_tag="PLY93_13715"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HRP70581.1"
                     /translation="MVTISILAGIAALLHFVFFLFESVLWTKPVVRKIFGQTEADART
                     TKLLALNQGFYNLFLALSTVAGIVLWHTGSIPAAVGILTVSLASMVGAAVVLFFSGGV
                     KMLRGALIQGLVPAVCLFLLWRPV"
     gene            503..973
                     /locus_tag="PLY93_13720"
     CDS             503..973
                     /locus_tag="PLY93_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HRP70582.1"
                     /translation="MTLTLLQEAATALGFSRVAALPIIQIEDEFRVFYEKYLTRGGHA
                     DLKYLERPERFTLTAIDPNAKTLLIFLYPYRFRSVETHLRATPYKIARYAWQKDYHYL
                     LKGKLNTLLEKFTGRGARSPILRRCWKNTGRGGQVWGLSGATAFSSTLHRGVIF"
     gene            913..>1283
                     /locus_tag="PLY93_13725"
     CDS             913..>1283
                     /locus_tag="PLY93_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRP70583.1"
                     /translation="MGFIGRNSLLIDPTSGSYFLIASLFIEEDLDADLPERNLYLFLA
                     NDFHAFCKDCTLCIDACPTGALLGDGLMNTQKCISYQSIETKEAAEYPKGTKKHRWIF
                     GCDVCQQVCPYNKNEESYSDE"
     assembly_gap    1284..1293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1502..>1879)
                     /locus_tag="PLY93_13730"
     CDS             complement(1502..>1879)
                     /locus_tag="PLY93_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRP70584.1"
                     /translation="EIAVREQVGLLAWSPLAAGLITGKYANGARPQGTRWAMQKKFNQ
                     RDTHEAHAAVDAYLKIAQTHQLDIAQMAIAFVLSRPFTTAAIIGATTMEQLKTNIAAC
                     DVMLSQEVMTDIQKVRRMYPMPF"
BASE COUNT          428 a          444 c          491 g          506 t
ORIGIN      
        1 gtgggtgtgg tttttatggc ctgactttta tatccgccgg atgaaaaagc gtgcatgaac
       61 aaccactatt ccttgacggg ataaatccca ccgctgaagc ttgtttatgg ttaccattag
      121 catactcgcc ggcatcgctg ctctattgca ctttgtgttt tttctttttg aaagcgtatt
      181 gtggacaaaa cctgtggtac gcaaaatttt cggtcaaact gaagccgacg cgcgcacgac
      241 gaaacttctc gcgctcaacc aaggctttta taatttgttt ttggcgcttt ctaccgttgc
      301 gggaattgtc ttgtggcaca ccggctctat cccggcggca gtgggaattt taaccgtgtc
      361 gctcgcgtcg atggtgggcg cggcggtggt gttgtttttt tcgggcggtg tgaaaatgct
      421 acggggtgcg ctcatccaag gccttgtgcc tgcggtgtgt ttatttcttt tgtggcgtcc
      481 cgtgtagcgc agcgttcgtg cgttgacttt aacacttctc caagaagcag caacagcgtt
      541 ggggttttcg cgtgttgccg cattgccgat aatccaaatc gaagatgagt tccgtgtttt
      601 ttatgaaaaa tacttaacgc gcggcggtca cgccgactta aagtatttag aacgccccga
      661 gcgttttact ttgactgcga ttgatcccaa cgcaaagacg ctcctcattt ttttataccc
      721 ataccgtttt cgtagcgtag agacacacct acgcgctacg ccgtacaaaa ttgctcgtta
      781 cgcgtggcag aaggattacc attatctctt aaaagggaaa ttaaacacac ttcttgaaaa
      841 attcactggc aggggcgcgc ggtcaccgat tctgcgccgg tgttggaaaa atactgggcg
      901 cggcgggcag gtttggggtt tatcgggcgc aacagccttc tcatcgaccc tacatcgggg
      961 agttattttt taatcgcatc gcttttcatt gaagaagatc tcgatgcgga tttacccgaa
     1021 cgaaacttgt atttattttt ggcaaacgat ttccatgcct tctgcaaaga ctgcacgctc
     1081 tgtatcgacg catgccccac cggtgcgctt ttaggcgacg gacttatgaa cactcaaaaa
     1141 tgtatttcat accaaagtat cgaaacaaaa gaagcggcag agtaccccaa aggcaccaaa
     1201 aagcaccgtt ggatttttgg ctgcgacgtt tgccaacagg tgtgtccgta caataaaaat
     1261 gaagaatcgt atagcgacga aatnnnnnnn nnntgtttaa cgatacacat cccgttatcc
     1321 cccttgtggc agcgggtgag gagaatttta cgcgctcgca gattaaaggc tcggttttgg
     1381 atcggcgtgg gatgaagaaa gtgcgcgaga ataaagaagc tctcaaaaaa atttaacgga
     1441 agcgcaacac aggaaagcat taacgccgct tcgactgtgg tatttctttt tcaccgtttg
     1501 gttaaaacgg catggggtac atacgccgca ccttttgaat gtcggtcatc acttcttgcg
     1561 atagcatcac gtcgcacgcg gcgatgttgg tctttaactg ttccatcgtc gtcgcgccga
     1621 tgatggcggc ggtggtgaag gggcgtgaaa gcacgaaggc aatcgccatt tgggcaatgt
     1681 cgagttggtg cgtttgggca attttcaagt acgcatcgac agcagcgtgc gcttcatggg
     1741 tgtcgcgctg gttaaatttt ttttgcatcg cccaacgggt gccttggggg cgcgctccgt
     1801 tcgcgtactt gccggtaatg aggcctgcgg ccaaaggcga ccacgcgagg aggcccactt
     1861 gctcgcgcac cgcaatttc
//
LOCUS       DAOMPD010000526         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23816_length_1865_cov_2.607182, whole genome shotgun sequence.
ACCESSION   DAOMPD010000526 DAOMPD010000000
VERSION     DAOMPD010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23816_length_1865_cov_2.607182"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1631
                     /locus_tag="PLY93_13735"
     CDS             117..1631
                     /locus_tag="PLY93_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70585.1"
                     /translation="MKSAVKIFGAADAIQKDVERLLDAQNKSYKQLSLNEKLTIFQHT
                     AVERASQSMGIEFHALRPYITDFMSFGASIYEHHHTMDVVKRASELGLTDTDLHTVDL
                     GGASLPAALELAQRICADEARLVLIAGSEIPRGGDPSIKYYREVSDTLLHPQKELHTH
                     ANLISLYALFADRMMFESNITVSDIERITEHYRSKATADERAAVFQKPLKAGELQRYL
                     ASPYATAMIAVATDHAIAFFAANQNYLNEVQQKLSSLKIENSLYIHSVGVSFVDKYVT
                     SRSNFTSPAQSAAARAFARSTLTPRHVDYAWVYDCFTLMLVKQAADYFNLPAQTVVKT
                     LQEGYIDVDGKKIHVNEQGGILNTQAAISLSAATGLIDIFQHAQKNNDANTFLFGGNG
                     GLDTVNAVAVLTREPVPINQNTIPPQPKKTAQPFNFTENQNATLYAATLVRFNPGSDV
                     PFVLGAFYDEDGSLFLARMTQKDGSPLQTTKNLVRDKTRVQISLKTGVPVARLY"
     gene            complement(1628..>1865)
                     /locus_tag="PLY93_13740"
     CDS             complement(1628..>1865)
                     /locus_tag="PLY93_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70586.1"
                     /translation="KAQANKYIFDFGNEFSLQGVFVPLSAGEYALFVTGTSAEAVNLK
                     ELSENLLPLGIRKVERVSHASFEQISAGAIDMSI"
BASE COUNT          524 a          523 c          380 g          438 t
ORIGIN      
        1 tttccctacg acacccagag gaaaaaagcc gcattcaagc cgtgcgaaaa atgccccatt
       61 tggtatggtg cgctcctcga acaactttcg ctcgctctcc cccgatttgc caaccgatga
      121 aatccgccgt taaaattttt ggcgccgccg atgccattca aaaagatgta gagcgccttc
      181 tcgacgcgca aaataaatcg tataagcaac tttcactcaa cgaaaaacta acaatctttc
      241 aacacaccgc cgtcgaacgc gcatcgcaaa gtatggggat tgagttccac gctctacgcc
      301 cgtatatcac tgactttatg agttttggcg cctcgattta cgaacaccac cacacgatgg
      361 atgtcgtgaa gcgcgcctct gaactgggac taaccgatac cgatttacac accgtggatt
      421 taggaggcgc gagtttaccc gccgcactcg aattggcgca gcgcatttgc gccgatgaag
      481 cacgcctcgt tcttatcgcg ggctctgaaa taccgcgcgg cggcgatccc agtataaaat
      541 actaccgcga agtttcggac actctgttgc acccgcaaaa agagctccac acacacgcca
      601 acttaatttc tttatacgca ctttttgccg accgcatgat gtttgaatcg aacatcaccg
      661 taagcgacat cgaacgcata accgaacact accgctcaaa agcaactgca gacgaacgcg
      721 ccgctgtttt tcaaaagcca ttgaaagcgg gcgaactcca acgctacctc gcgtcacctt
      781 atgcaacggc aatgatcgcg gttgccaccg accatgcaat agcttttttt gcggcaaacc
      841 aaaactactt aaatgaagtt cagcaaaaac tttcttctct taaaatagaa aattcactct
      901 acatccattc tgtcggtgtg agcttcgtcg ataagtacgt taccagtcgt agcaatttta
      961 cttcgcccgc ccaaagcgca gccgcccgtg catttgcccg ctcgacactc acgccgcgcc
     1021 atgtcgatta cgcgtgggtg tacgattgct ttactcttat gctcgtaaaa caagccgccg
     1081 attattttaa tctccccgca caaactgtgg taaagactct ccaagaaggg tatatagatg
     1141 tagacggaaa gaaaatccat gtcaacgaac aaggtggtat tttgaatacc caagcggcga
     1201 tttccctttc cgccgccacg gggttgatcg atatttttca acacgcgcaa aaaaataacg
     1261 acgccaacac atttcttttt ggtggaaacg gcggcctaga cacggtgaac gccgtcgcag
     1321 tactcacgcg cgaaccggtg cctattaacc aaaataccat cccaccgcaa cctaaaaaaa
     1381 ccgcgcaacc gtttaatttc acagaaaatc aaaacgccac gctttatgcc gccacactgg
     1441 tgcgctttaa cccagggagc gatgtgccat ttgttctcgg cgcgttttac gacgaagacg
     1501 gatcgctttt tcttgcacgt atgacgcaaa aagacggttc gccgctacaa acgacaaaaa
     1561 atcttgtacg cgataaaacg cgcgttcaaa tttcactcaa aaccggcgta cctgttgcga
     1621 gactttatta aattgacata tcgatagcac cggcagaaat ttgttcaaac gaagcatggc
     1681 tcacccgctc gactttgcgt ataccgagtg gcaataaatt ctcactcagt tctttaagat
     1741 taaccgcttc ggcgcttgtg cccgtcacga aaagtgcata ctcacccgcg cttaaaggta
     1801 caaaaactcc ttgcaaagag aactcattcc caaaatcaaa aatatattta ttggcttgtg
     1861 cttta
//
LOCUS       DAOMPD010000527         1864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23835_length_1864_cov_2.053068, whole genome shotgun sequence.
ACCESSION   DAOMPD010000527 DAOMPD010000000
VERSION     DAOMPD010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23835_length_1864_cov_2.053068"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>616)
                     /locus_tag="PLY93_13745"
     CDS             complement(<1..>616)
                     /locus_tag="PLY93_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP70587.1"
                     /translation="SHFARGGESIEPIFLTQVFDRNKKLLRDFTPKQRKEQIVSAGTA
                     SIVRSLLETAVNQGTGSSVRKVGYRGYAAGKTGTTNGYRDAWFVGFNKRYTAAVWVGY
                     DRPSLSLGPGQAGAGVAAPIWSNYQIRLQEKIKDEEPYLKSQALTPITLCRGSGTPAA
                     QAPSCAETYTEYFLPDTGPETGNKIDLGDSVSIDTHIDTGSVNPP"
     assembly_gap    617..634
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(646..>1864)
                     /locus_tag="PLY93_13750"
     CDS             complement(646..>1864)
                     /locus_tag="PLY93_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRP70588.1"
                     /translation="WRCICAGYVHQERSACGRVTENFVTGSEFYFESHVKNLTVAQAA
                     MLATLPSSPNRYSPIRNPHVSRVRLQHALMKMVDLGFIDIQTAQKADVEAVQYYTTLN
                     IAPSETAYGRRVDNAPYFSEWVRPLLEKEFGKDALYNEGLKIHTGLDLDHQKIAQNQL
                     WRALREQNSISRNYFFSKQLELSHEYSALLTLSQFALNLPELNPSRRFAQYEQQMVFF
                     EEFLDPLELVNLGFGGDERLDALLLRLRADNPFANRYLAVQGAFVELDNKTGEVTAVV
                     GGLPFNTQNQMNRALQMQRQPGSSFKPLLYAAAIETKKVTAATQFPDSPIVSIDERGD
                     LWMPENYSSGFRGFITLRDALRYSVNTVSIALARDVGLSNVIPIIANELYVKPQQIPY
                     NLSIALGTYEVSP"
BASE COUNT          443 a          451 c          476 g          476 t
ORIGIN      
        1 gggggattta cactgccagt gtcgatatgg gtgtcgatac ttacgctatc ccccaaatca
       61 attttgttac cggtttctgg accggtgtcg ggcaaaaaat actcggtgta cgtttcagcg
      121 cacgagggtg cctgcgctgc gggtgtaccc gagcctcggc aaagcgtaat tggcgtgagc
      181 gcttgcgatt tcaaataggg ttcttcgtct ttaatttttt cttgaaggcg tatttggtag
      241 ttcgaccata tcggcgcagc cacccctgcc cccgcttgac caggccccaa cgaaaggctg
      301 ggtctatcgt accccaccca caccgctgcg gtataacgct tattaaaccc tacgaaccac
      361 gcatcgcggt agccgttggt ggtgcctgtt tttccggcgg cataccctcg gtagcctact
      421 ttgcgcacac tcgagcccgt gccttggttg accgccgttt caagtagaga acgcacgatg
      481 ctggcggtac cagcgctcac aatttgttct tttctttgct tgggtgtaaa atctcttagc
      541 aattttttat tgcggtcaaa aacttgcgtt aaaaaaatcg gctcaatgct ttcgccgcca
      601 cgcgcaaagt gtgaaannnn nnnnnnnnnn nnnncgcgcc atcccttaag gcgaaacttc
      661 ataagtgccc aaagcaatcg acaagttata cggtatttgt tgcggtttaa cgtaaagttc
      721 atttgcaatt atggggatta cattgcttaa acccacgtcg cgcgccaacg cgatactgac
      781 ggtatttacc gaatagcgta acgcatcgcg taacgtgata aatcctctaa agcccgatga
      841 ataattttcg ggcatccata aatcgccgcg ctcgtcgata ctcacgatgg gcgaatcggg
      901 aaactgggtt gccgccgtta cttttttggt ttcaatcgca gcggcgtaaa gaagcggctt
      961 aaaagacgac ccgggttggc gctgcatttg taaggcacgg ttcatttggt tttgggtgtt
     1021 gaatggcaaa ccgcccacta ccgccgtcac ttcgcctgtt ttattatcaa gctcaacgaa
     1081 cgcgccctgt actgcgaggt agcggttggc aaaggggttg tcagcgcgca agcgcagaag
     1141 gagtgcatcg agtctttcgt cgccgccaaa acccaaattg acgagctcta acgggtctaa
     1201 aaattcttca aaaaaaacca tctgctgttc gtactgagca aaacggcgac tgggattaag
     1261 ctcgggcaag ttcaatgcaa actggctcaa ggttaaaagc gccgaatact catgcgagag
     1321 ttcgagctgc tttgaaaaaa aataattgcg tgaaattgag ttttgctcgc gcaaggcgcg
     1381 ccaaagttgg ttttgtgcaa ttttttggtg atctaaatcg aggcccgtgt gaatttttaa
     1441 accttcgttg tagagtgcat ctttgccaaa ttctttttct aataatgggc gtacccactc
     1501 cgaaaagtac ggagcattat cgacacggcg cccatacgcg gtttcggagg gagcaatatt
     1561 gagggtggtg tagtattgta ccgcttcgac gtcggccttt tgcgcagttt ggatgtcgat
     1621 aaaacccaag tcgaccattt tcataagagc gtgttgcaaa cgcacgcgtg agacgtgagg
     1681 attacggatg ggtgaataac ggttgggcga actgggcaag gttgcgagca tggccgcttg
     1741 cgcaacggtg agatttttta cgtgactttc aaaataaaat tcggagccgg taacgaaatt
     1801 ctcggtaaca cggccgcagg cgctcctttc ctggtgtaca tacccggcac agatacaccg
     1861 ccac
//
LOCUS       DAOMPD010000528         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23883_length_1862_cov_2.156060, whole genome shotgun sequence.
ACCESSION   DAOMPD010000528 DAOMPD010000000
VERSION     DAOMPD010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23883_length_1862_cov_2.156060"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>478)
                     /locus_tag="PLY93_13755"
     CDS             complement(<1..>478)
                     /locus_tag="PLY93_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRP70589.1"
                     /translation="GATEKFIGTWFQARKNRDKVILASKVAGNGTTWMHDGSDINRVS
                     LLSSIDGSLKRLQTDYIDLYQLHWPNRGSYHFGQLWNYKTKRNAKEEELENFLEVLTT
                     LNSLVQSGKIRYIGLSNETAWGTMQYLRLAETQNLPRVQSEQNEYSLLYRLHEGDMH"
     assembly_gap    479..488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<489..556)
                     /locus_tag="PLY93_13760"
     CDS             complement(<489..556)
                     /locus_tag="PLY93_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70590.1"
                     /translation="MDYALSQGINFFDTAEMYPVPAS"
     gene            complement(665..1777)
                     /locus_tag="PLY93_13765"
     CDS             complement(665..1777)
                     /locus_tag="PLY93_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRP70591.1"
                     /translation="MRSISIFTAVLFVYASAILAAPQTQNSDAARVAIIKFGDKTGTQ
                     NFEYMPGSLQDAITNSMKKKFEFIEVDPTKVEPIAEKIRKANKGQIGPKEAAEICRLA
                     DIDILIYGNFTFSEETKTIEINTQISLGSTDKYRTTKPVQNRVDATIFGAADKVAGDI
                     VSEITLVAKEQQAAKGMEAEKNKKGKTQLAKTEKTITWADIRWNFSADAGAGYPLVSA
                     KYANIRIQPLIGLTAMYRWRNNWHIGGQIFMDSFYSFEKNGPNATYIQFFTPAANLGY
                     FFDLSPSWRATTTVGLGYYVGKFDNHAECNSNCYIQTTSPTKKTIYNPFVTVAAGIHY
                     LVFSHFSVGLNARYNMFYDGKPLHAVSGTLSLSLLF"
BASE COUNT          437 a          383 c          472 g          560 t
ORIGIN      
        1 cgtgcatgtc gccctcgtgc aggcggtaga gaaggctgta ttcgttttgt tccgattgca
       61 cgcgaggcag gttttgggtc tcagcaaggc ggaggtattg cattgtcccc caagcggttt
      121 cgttggaaag accgatgtac cggatcttcc cagattgaac gagcgagttt aacgttgtaa
      181 gtacttctaa aaaattttca agctcttctt ctttggcgtt acgcttagtt ttgtaattcc
      241 ataattggcc aaaatggtag ctgccacggt taggccaatg caactgataa aggtcgatgt
      301 aatcggtttg tagtctttta agtgagccgt cgatcgaact taaaagactt acgcgattga
      361 tgtcgctgcc gtcgtgcatc cacgtggtgc cattgcccgc gactttgctt gctaagatga
      421 ctttgtcgcg atttttacgc gcctgaaacc aagtaccgat aaatttttca gtggcgccnn
      481 nnnnnnnnga agcgggcacg gggtacattt cagccgtgtc gaaaaaattg atgccttggc
      541 tgagcgcata gtccatttgg gcatgcgctt cctcctcggt gttttggatg ccccacgtca
      601 tcgtgccgag gcaaatgctg ctgacttgaa gtgaggtgtt gccgagattg cgatagttca
      661 tcctctaaaa caaaagcgac aagctcagcg ttccagaaac ggcgtgtagt ggtttgccgt
      721 cgtagaacat attatagcgg gcgttgagtc ccaccgaaaa atgcgaaaag actaagtagt
      781 ggataccagc ggcaactgtg acaaaggggt tataaattgt ttttttagtg ggggaggtgg
      841 tttggatata gcagttggag ttacactctg catggttgtc aaatttaccg acgtaatagc
      901 ctaaccccac cgttgtggtt gcacgccaag agggggataa atcaaagaag taacccaagt
      961 tagcagcggg ggtgaagaac tgtatatatg tggcattagg gccatttttc tcaaaagagt
     1021 aaaaagagtc cataaatatc tggccaccaa tatgccagtt attgcgccag cggtacatcg
     1081 ccgtgagacc tatgagtggc tggattctta tatttgcata ctttgcactc acgagtgggt
     1141 atcccgctcc agcatctgct gaaaaattcc atcggatgtc cgcccaagtt atggtttttt
     1201 ctgtttttgc caattgagtt tttccctttt tgtttttctc cgcttccata cctttggcgg
     1261 cttgttgttc tttggcgacg agggtaattt ctgaaacgat gtcgccggcg actttgtcgg
     1321 cagcaccaaa gatggtggcg tcgactcggt tttgcaccgg ttttgtcgtg cggtatttgt
     1381 cggttgagcc taaagaaatt tgtgtgttaa tttcgattgt ttttgtttct tcgctaaatg
     1441 taaaatttcc gtagatgaga atatcgatgt cggcaaggcg acaaatttca gcggcttctt
     1501 tgggaccaat ttggcctttg ttggcttttc taattttttc agcaatcggc tctacttttg
     1561 tagggtcgac ttctataaac tcaaattttt tcttcattga gtttgtgatt gcatcttgta
     1621 acgaccccgg catatattca aagttctgag taccggtctt gtctccaaat ttaatgatcg
     1681 caacccgtgc ggcgtcgcta ttttgtgttt gcggggcggc aaggatggct gacgcataca
     1741 caaagagaac agcggtaaaa atagaaatcg aacgcatttg tttttataga agtttattgt
     1801 atcgcgtcaa tcgtaaaaac ttaccctcgg cggggaggtc ggacagattt ttaggaaaat
     1861 cg
//
LOCUS       DAOMPD010000529         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_23962_length_1857_cov_2.680910, whole genome shotgun sequence.
ACCESSION   DAOMPD010000529 DAOMPD010000000
VERSION     DAOMPD010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23962_length_1857_cov_2.680910"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLY93_13770"
     CDS             <1..289
                     /locus_tag="PLY93_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRP70592.1"
                     /translation="EEAATRGAGEILLTSMDADGAKTGYDLVVTKNIAEKVSIPVIAS
                     GGAGKKEDFAAAFRAGANAALAASLFHFNELKINELKEYLVHEGFPIRANV"
     gene            371..934
                     /locus_tag="PLY93_13775"
     CDS             371..934
                     /locus_tag="PLY93_13775"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRP70593.1"
                     /translation="MQDIDFSRYYRRGRRPNEYSVSDNREKTGNFLPANKLSLYLAIA
                     FSCLAIGFIGGTQLQKSKMTREIENKVVPESMATANVAEPSVETEKQMESVRVTRTED
                     KIVAENTVHEAKPNYLILAKIFPNEKEASLAGLGLKRNGYRAFMARNGKKIKLYVGPI
                     EGKSEAYDALAKIKKNAEFSGAILYRK"
     gene            982..1452
                     /locus_tag="PLY93_13780"
     CDS             982..1452
                     /locus_tag="PLY93_13780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRP70594.1"
                     /translation="MLVVRDAVIDDAASIAALLKPYVEKKNLLHRSLEEIRANIDKTI
                     VVVEDERIVGTTSLVFFSPTLCEVRALVVAEDLQRGGVGRLLVEAAEEKAQQMRTTRP
                     LRVFALTYTPEFFERLGYARTTKDRFPEKIYEVCRFCLRKDDCHEIAVEKIFSV"
     gene            1616..>1857
                     /locus_tag="PLY93_13785"
     CDS             1616..>1857
                     /locus_tag="PLY93_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70595.1"
                     /translation="MNVLRKLAPFFLIGLILTGVVVGTVFTFKFFNRANLEKALASSD
                     GFSLLFVVTSAIDDKKIQVISVAEIFPATRRIGVVS"
BASE COUNT          560 a          360 c          419 g          518 t
ORIGIN      
        1 agaagaggcg gcaacgcgcg gtgcgggtga aattcttttg acttcgatgg acgccgacgg
       61 cgcaaaaacg gggtatgatt tagtcgtcac aaaaaatatt gccgaaaaag tttcgatccc
      121 tgtcatcgct tcggggggcg cgggtaaaaa agaagatttt gctgcagcat tccgtgcggg
      181 cgctaacgct gcgcttgcgg cgtcgctctt ccatttcaac gaacttaaaa tcaacgagtt
      241 gaaagaatat ttagtccacg aaggatttcc catccgtgca aacgtttagc tttgtggtaa
      301 aactatctta aaatcgcgca agcggtaaaa tagtgctaca agaattgtgc aatatgcacg
      361 aaaattaaat atgcaggata tcgattttag ccgttattat cgtcgtggac ggcgccccaa
      421 tgaatactcg gtttcggata atcgagaaaa aacgggaaat tttttacccg ccaataagct
      481 ttccctttat ttagccattg ctttctcatg ccttgcaatt ggttttattg ggggtactca
      541 attacaaaaa agtaaaatga ctcgggagat tgagaacaaa gttgttcccg aaagcatggc
      601 aacggcgaac gtcgccgaac caagtgtaga gactgaaaag caaatggaaa gcgtgcgagt
      661 aactcgcaca gaagataaaa ttgtagcaga aaatactgtg catgaagcaa agccaaacta
      721 tttgattctt gctaaaattt ttccaaacga aaaagaagcg tcgctcgcag ggttaggctt
      781 aaagcgcaac ggataccgag cgtttatggc gcgcaacggt aaaaaaatta agctgtatgt
      841 aggccccatc gaaggtaaaa gcgaagcgta cgacgcttta gcaaaaataa aaaagaatgc
      901 cgagttttcg ggcgcaatac tttaccgaaa ataaataaaa cacatttatt tttacaacgc
      961 aagtaagatt tttaaaacgt aatgcttgtg gttcgtgacg cagtgatcga cgacgcggcg
     1021 agtattgctg cgttacttaa accttatgtc gaaaaaaaaa atctattgca ccgttcgtta
     1081 gaagaaattc gagcgaacat cgacaaaact atcgtcgttg ttgaagacga gcgcatcgtc
     1141 ggcacaacga gtttagtttt tttctctccg acgctttgcg aagttcgtgc gttggtggtt
     1201 gcagaggatt tacaacgcgg cggcgtgggt cgtcttctcg ttgaagcggc agaagaaaaa
     1261 gcgcaacaga tgcgaacaac aagacctctt cgagtttttg cgctcacgta cactccagaa
     1321 ttttttgaac gcttaggtta tgcgcgcaca acgaaagatc gctttccaga aaaaatttat
     1381 gaagtgtgtc gtttctgttt acgcaaagac gattgccatg aaattgcagt agagaaaatt
     1441 ttttcagtgt aaaatttatt tttgaaaaat atttttaaaa attattttcg gcgcatgaaa
     1501 aattttttac gtaaatgatt gacgccccac gtttgttgaa tacgatgtga cataattttc
     1561 aatgcgacat aatgttgtta cagaaaatcg atttgagtta cgataagaac aattcatgaa
     1621 cgtattacgt aaactcgctc cctttttttt aatcggtctt atacttaccg gcgttgtcgt
     1681 tggcactgtg tttacattca aattttttaa ccgcgctaat ttagaaaaag cattggcgtc
     1741 gagcgatggt ttctcgcttc tctttgttgt aacctcggcg atcgatgata aaaaaattca
     1801 agtcatttcc gtggctgaaa tttttcctgc gacgcgccgc atcggtgtcg tttcttt
//
LOCUS       DAOMPD010000530         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24044_length_1852_cov_2.634947, whole genome shotgun sequence.
ACCESSION   DAOMPD010000530 DAOMPD010000000
VERSION     DAOMPD010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24044_length_1852_cov_2.634947"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="PLY93_13790"
     CDS             complement(<1..1532)
                     /locus_tag="PLY93_13790"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRP70596.1"
                     /translation="MKKTRYIFVTGGVASSLGKGVSIASIGALLEARGFKVTIQKMDP
                     YINIDPGTMSPFQHGEVYVTEDGAETDLDLGYYERFTSATLGRENSVSTGQIYHSVIS
                     RERRGEYLGRTVQVVPHITNEIKNRILLLAQKDTTLDFVLVEIGGTVGDIESVPFLEA
                     IRQFRLDQGPENSMFIHLTLVPSIVDGGEIKTKPTQHSVKELLKHGIQPDILLCRTTT
                     MLDADVKEKISLFCNVAKTRVFSAPDIEHTIYEIPQIFHSEDLDKEIIRYFRMENQSG
                     EFLQNLNPMLAKWNQVVDAFKNPRQRVKIAVVGKYMALHDAYRSLYEAMTHGGFHKHI
                     AVEFIKIDSEKLEEENYEAELKDAQGILVPGGFGERGVEGKLLAIRYARTKKIPYFGI
                     CLGMQCAVIEFARNVLNFTDAHSTEFAIDTTHPVIHLMEEQSAVVNKGGTMRLGAYPT
                     KIREGSLLAECYKEPLVQERHRHRFEFNNKYRAAFENAGLKISALSPDEKLVEAVEID
                     TA"
BASE COUNT          464 a          432 c          449 g          507 t
ORIGIN      
        1 tttgccgtgt cgatttccac cgcctcgaca agtttctcgt cgggggaaag agcagagatt
       61 ttgagccctg cgttctcaaa cgcagcacgg tatttattgt tgaactcaaa gcgatggcgg
      121 tggcgttctt gtaccaacgg ttctttatag cactcggcga ggagtgagcc ttcgcggatt
      181 ttggtggggt atgcccccaa acgcatcgtg ccgcctttgt ttaccactgc cgattgttct
      241 tccataagat gaatcaccgg gtgcgttgta tcgatggcaa attcggtcga gtgcgcgtcg
      301 gtaaaattta agacgttgcg cgcaaactca atcaccgcgc actgcatccc caagcaaata
      361 ccaaagtaag gaattttttt agtgcgtgca taccggattg ccaagagctt tccctcgacg
      421 ccgcgttcgc caaaaccgcc gggtactaaa atcccctgag cgtcttttaa ttctgcttcg
      481 taattttctt cttcgagttt ttcagaatcg attttaataa attcgacggc gatgtgtttg
      541 tgaaaaccgc catgcgtcat cgcttcgtag agcgagcggt acgcgtcgtg cagcgccatg
      601 tatttaccca cgacggcaat cttcacgcgt tggcgcggat tcttaaaagc atcgacaact
      661 tggttccatt tggcgagcat cgggttcaag ttttgtaaga actcccccga ctggttttcc
      721 atacggaaat aacggataat ctctttgtct agatcttcac tatggaaaat ttgtgggatt
      781 tcataaatcg tgtgttcgat atcgggggcc gaaaaaacgc gcgttttggc gacgttacaa
      841 aaaagagaaa ttttttcttt cacatcggcg tcgagcatcg tggtcgtgcg gcacaaaaga
      901 atgtcgggtt ggatgccgtg cttcaaaagc tcttttaccg agtgttgcgt cggctttgtt
      961 tttatctccc ctccgtcgac aatcgacggc accagcgtga ggtggataaa cattgaattt
     1021 tcagggcctt ggtcgagtcg gaattgacga atcgcttcta aaaaaggtac cgattcgatg
     1081 tcgcccactg tgccgccgat ttccacgaga acaaaatcga gagtcgtgtc tttttgcgcc
     1141 aagagtaaaa tacggttttt aatttcattg gtgatatgcg gcaccacttg taccgtgcgt
     1201 cccaaatact cgccccgccg ctcgcgtgaa ataacgctat ggtaaatttg tccggtggag
     1261 accgagttct cccgccccaa tgtcgcactg gtgaaacgct cgtagtaccc caagtcgagg
     1321 tcggtttcgg cgccgtcttc ggtcacatag acttcgccgt gctgaaacgg actcatcgtg
     1381 cccgggtcga tattgatata cgggtccatt ttttggatag ttaccttaaa tccacgcgct
     1441 tccaggagtg cgccgatgga agcgattgaa acgcctttgc ccaaggaact ggcaacaccg
     1501 ccggtaacaa aaatatagcg tgtttttttc atattaataa gaccttaccc ctgcacaaac
     1561 tatcggatta tgtcgcatcg acaaatggcg cgtcaagtta ttgagattgt ctacaaaatt
     1621 aagaacttta tggacatcgc tgtctatttt tagtattgta tagatataga ttatataaag
     1681 gagaatacaa tgccgcaatt aaaattatta aaaaaacaag atgaagttga aaatatcaag
     1741 acgtttacct ttgaaacggg gggagcgtcg tggacgcctg ggcagtatca agcgtatgag
     1801 cttaaacaag cgggcagcga tgaaaaagag cgtattcgct atttcaccat cg
//
LOCUS       DAOMPD010000531         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24068_length_1851_cov_2.285078, whole genome shotgun sequence.
ACCESSION   DAOMPD010000531 DAOMPD010000000
VERSION     DAOMPD010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24068_length_1851_cov_2.285078"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLY93_13795"
     CDS             <1..505
                     /locus_tag="PLY93_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70597.1"
                     /translation="SAESVQIGKKQAHWFKITNAAGLTGWAYGAGLAVESDEGNVQDI
                     QAKAEKKLRDAMIGRWEAATIQGALTPNFVALYTDGKIEFGINRSKTQTGKYEIIFDG
                     ANAQVIVSDIKNPMMTDIKAKMVGETLVFEAVKDGKDYKLNLADKDPGGLEAEKKAKT
                     DAATTAP"
     gene            506..1234
                     /locus_tag="PLY93_13800"
     CDS             506..1234
                     /locus_tag="PLY93_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRP70598.1"
                     /translation="MQRTNSNTNKIRIGKVLAGLGVAPRRQTADFLAQHDVRIDGEKI
                     TELNYAVDANKIPLLNLTIDGKRIAMEFHSDKVLLFHKPTGVVSSHRTQKIRGKELKT
                     IYDFLPVKYKTWFFAGRLDISSSGLMVLSNDGDHIFQLSHPKFGALKKYFVRTSRPLS
                     NDEIIKIQKGVFDKGEKLRFEKCIALDKPAHYEIHLKEGKNREIRRVLEKVGVFAREL
                     RRTELGPYALETIPVGGFLLTEKK"
     gene            1277..>1851
                     /locus_tag="PLY93_13805"
     CDS             1277..>1851
                     /locus_tag="PLY93_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRP70599.1"
                     /translation="MIEVTVREALNTALTEELARDKDVFLIGEEVGHYNGAYKVSQHL
                     MERFGEDRVIDTPIAEAGFAGLAIGAAMAGLRPIVEFMTWNFSLVAIDQLINNAAKMH
                     FMSGGQFPLAIVFRAPQGAGGSLAAQHSTVLEGFYAYTPGLIVIAPSTVGDMRGLLKS
                     AVRENNPVVFLEHEKIYAVKGELEHADNPDY"
BASE COUNT          521 a          430 c          474 g          426 t
ORIGIN      
        1 cagcgccgag agcgtgcaaa tcggcaaaaa gcaagcgcac tggtttaaga tcaccaacgc
       61 tgcgggttta accggttggg catacggcgc cggtttagcg gttgaatccg acgaaggtaa
      121 cgtccaagat atccaagcta aagcagaaaa aaaattgcgc gatgccatga ttggtcgttg
      181 ggaggctgcc accatccaag gagcgcttac ccccaatttt gtggcgctct ataccgacgg
      241 aaaaattgag tttggtatca accgttcaaa aacacaaacc ggtaaatatg aaatcatttt
      301 tgatggagcc aacgcgcaag tgattgtgag cgatattaaa aaccccatga tgaccgacat
      361 caaagccaaa atggtgggcg agacattggt ttttgaagct gtcaaagacg ggaaggatta
      421 caaactgaat ttggcagata aagaccctgg tggtttagag gccgaaaaaa aagcaaaaac
      481 cgacgccgca acgacggcac cgtaattgca aaggacaaat tcaaacacga ataaaatccg
      541 tatcggtaaa gtcttggcgg gcttgggagt tgcgccgcga cgccagacgg cggatttctt
      601 agctcagcac gatgtacgca tcgatggcga aaaaattact gagctcaact atgccgtcga
      661 tgcaaataaa attccgctgt taaacctcac catcgacggc aaacgtatcg ctatggaatt
      721 ccatagcgac aaagttttgc tgttccataa acccaccggc gtggtctcgt cacatcgcac
      781 gcaaaaaatc cgtggcaaag aacttaaaac catttatgat tttctgcccg taaaatacaa
      841 aacttggttt tttgcgggca ggctcgacat tagctcgtcg gggctcatgg tactctccaa
      901 cgacggcgat catattttcc aattaagcca tcccaagttt ggcgcgctaa aaaaatattt
      961 tgtacgcacc tcgcgcccac tttccaacga cgaaattata aaaattcaaa aaggcgtgtt
     1021 cgacaaaggc gagaaactac gctttgaaaa gtgcatcgca ctcgataaac cagcacatta
     1081 cgaaattcat cttaaagaag gtaaaaaccg cgagatacgc cgcgtgcttg aaaaagtggg
     1141 tgtgtttgcg cgcgagttac ggcgcaccga gttgggaccg tacgcgctgg aaacgatacc
     1201 ggtgggtggg tttttattga cggaaaaaaa atgaataacc gcttgcccgc tgcttaattt
     1261 ttgtgcaaag ggttctatga ttgaagttac cgttcgcgaa gcgctcaata ccgcacttac
     1321 cgaagagctc gcgcgcgaca aagatgtttt cttgattggc gaagaagtgg ggcattataa
     1381 cggcgcgtat aaagtttcgc agcacctcat ggaacgcttc ggcgaagacc gcgtgatcga
     1441 tacaccgatt gccgaggcgg gttttgcggg gctcgcaatc ggcgctgcga tggctggcct
     1501 gcgcccgatt gtggagttca tgacatggaa tttttcgttg gtagcaattg accaactcat
     1561 caacaacgcg gcgaagatgc actttatgtc ggggggacaa ttcccgcttg cgattgtttt
     1621 tcgcgcgccg caaggagcgg gtggttcgct cgccgcgcaa cactcgacgg tgttggaagg
     1681 tttttatgcg tacacgcccg gtcttattgt cattgctcct tcgacggtcg gcgatatgcg
     1741 cggactttta aaatcggcgg tgcgcgagaa taatcctgta gtgtttttag aacacgaaaa
     1801 aatttatgcg gtcaaaggcg aactcgaaca cgccgataat cccgattatt t
//
LOCUS       DAOMPD010000532         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24143_length_1847_cov_1.779576, whole genome shotgun sequence.
ACCESSION   DAOMPD010000532 DAOMPD010000000
VERSION     DAOMPD010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24143_length_1847_cov_1.779576"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>376
                     /locus_tag="PLY93_13810"
     CDS             <1..>376
                     /locus_tag="PLY93_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70600.1"
                     /translation="GENDRWNDEFQYFSRNDSGKLPGLNGDVSTYRTGLAVSGEPFMK
                     KLLKMRLDPRVYAYYARVRGNNGGSDYSENGRIGNFSDNDWSLLTGARLAWNGQEQFP
                     FFRQLQVYVDGALSTGSDLKRQG"
     assembly_gap    377..386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <387..1042
                     /locus_tag="PLY93_13815"
     CDS             <387..1042
                     /locus_tag="PLY93_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70601.1"
                     /translation="ASFGGGAGVSAQTKSILGFLSPLGELDFFFAQGAKYDKNGNMTS
                     HGFIGMRGDRIGGTLMRRYWGVRPSGYVNYGGIDDTPFDANRKAGSLLLHAGVGAEMW
                     KRFVVRADIWHLQDTGSTSVDFSPTNLDLINNPYVSRAELEAQKRLGKTLGQEINLTI
                     QYFPNSLLMFQLTASLFVPGSFFDTAVEDIVSKNGVPKGGPADADFMGVFLRSTISF"
     gene            1042..1827
                     /locus_tag="PLY93_13820"
     CDS             1042..1827
                     /locus_tag="PLY93_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70602.1"
                     /translation="MGRSIPHFYLFVLALAAVSFSACTSSVGEPWPTAINVDRKRGCA
                     EIREVNWGGSMKNDGTYDADDDFIEIQNSDCNKPVDLTDWRIELSGDIHKIFYIPAGA
                     NNTIAPGGFKVIVGKASGAFRDQGSADYQPIVLPDLAIPERNWAITTRTAENFLMESG
                     INTTEADDSTSRNFPLSGAFDGYTVRSMERTEDTFEEEGGSVSTWHASTPCNETSPSG
                     QGTPLMGTACSTTSNGASGKNVHADYRLRTFATPGEKNTPDYK"
BASE COUNT          456 a          471 c          472 g          438 t
ORIGIN      
        1 ggtgaaaacg atcgctggaa cgacgagttc cagtatttct cgcgcaacga cagcggtaaa
       61 cttcccggtc tcaacggaga tgtctcgacg taccgcaccg gtcttgcggt gagcggcgag
      121 cctttcatga aaaaactttt gaagatgcgc cttgacccac gcgtgtatgc gtattatgca
      181 cgcgtacgcg gtaacaatgg cggttccgat tattctgaaa acggacgcat tggcaatttt
      241 tccgataacg actggtctct cctcaccggc gctcgtcttg cttggaatgg gcaagagcaa
      301 tttccatttt ttaggcagct tcaagtgtat gtcgacggcg ctctttccac cggttccgac
      361 ttaaaacgac aagggcnnnn nnnnnnacgc atcattcggc ggtggcgcag gggtgtcggc
      421 gcaaacaaaa agcatattgg gttttttatc ccctttggga gaactcgact ttttctttgc
      481 acaaggcgca aaatacgaca agaatgggaa catgacctcg cacgggttca tcggtatgcg
      541 aggtgaccgc atcggtggca ctctaatgcg ccgctattgg ggagtgcgcc cttcggggta
      601 cgttaactac ggtggcatcg acgacacccc gttcgatgca aaccgtaaag caggctctct
      661 cctcctccac gcgggggtcg gcgccgaaat gtggaagcgt tttgtggtac gtgccgatat
      721 ttggcatttg caagacaccg gttcgaccag cgttgatttt tcgccgacaa acttggatct
      781 catcaacaac ccttatgttt cgcgcgcaga actggaggct caaaaacgct tagggaaaac
      841 tttggggcaa gaaatcaatc tcaccatcca atacttcccc aactcgctcc tcatgttcca
      901 gctcaccgct tcgctatttg taccgggttc gttctttgat actgcggtag aagacattgt
      961 gagtaaaaac ggcgtcccca aaggcggtcc cgctgacgct gatttcatgg gtgttttctt
     1021 aaggagcacg ataagtttct aatgggacgc tcaattccac atttttattt atttgttctt
     1081 gcattggctg ctgtaagctt tagcgcgtgt acctcatcgg tgggcgaacc gtggcccact
     1141 gcgatcaacg tcgacaggaa acggggctgt gcagaaatcc gtgaagtgaa ttggggaggc
     1201 tcgatgaaaa acgacggcac ttacgatgcc gacgacgatt ttattgaaat ccaaaattcg
     1261 gattgcaaca agcccgtcga tttaaccgac tggcggattg aactttcagg ggatatacat
     1321 aaaatttttt atataccagc cggagcaaat aacaccatcg caccgggtgg cttcaaagtg
     1381 attgtcggta aagcaagcgg cgctttccgc gaccaaggga gcgccgacta ccaaccaatt
     1441 gtgctccctg atctagcaat ccccgaacgc aactgggcga taaccacacg caccgctgaa
     1501 aattttttga tggaaagtgg gatcaacacc accgaagccg acgactccac cagtcgcaac
     1561 tttccgctct ccggcgcgtt cgatggttat accgtgcgat cgatggaacg caccgaagat
     1621 acgtttgaag aagagggggg atcggtttcg acgtggcatg cgtcgacgcc gtgtaacgag
     1681 acttctcctt caggacaagg tacgccactt atgggaactg cttgttccac aacatcgaat
     1741 ggcgcgtcgg gaaaaaatgt ccatgccgat taccgtttgc gtacgtttgc cacccctggc
     1801 gaaaaaaata caccggacta caaataatga gaatctcgaa aacatat
//
LOCUS       DAOMPD010000533         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24171_length_1845_cov_2.415642, whole genome shotgun sequence.
ACCESSION   DAOMPD010000533 DAOMPD010000000
VERSION     DAOMPD010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24171_length_1845_cov_2.415642"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLY93_13825"
     CDS             complement(<1..481)
                     /locus_tag="PLY93_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRP70603.1"
                     /translation="MSFNRAAVDSELLKEFFIGCEEHLSEAESAFIDLEKHPSDIDII
                     NDIFRHFHSVKGDAAAIGVDAVRILAHELETMLDKLREQTVTTTPELLDILLEGLTLL
                     QSQVLAIQENKPLPDIDGILTKLSSYTAHEKSGDTMQSAPTSAALDDMLVEETNSAAQ
                     "
     gene            complement(478..>1561)
                     /locus_tag="PLY93_13830"
     CDS             complement(478..>1561)
                     /locus_tag="PLY93_13830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRP70604.1"
                     /translation="DQARRELDQYFAGKLRDLALGIRMVPIGQTLKKYPAAVRDMARK
                     QHKEIRVIIEGEETELDKAILEEISDPLLHMIRNSVDHGIEMPDVRVKNGKSREGTLL
                     IRAEHEGDRVSIFVEDDGGGLNTQKILAKALEKGIVSAKTAENLSEREIFQLIFAAGF
                     STADKVSELSGRGVGMDVVKNNILRLNGTVDVESAQGVGTRITLKLPLTLSILEGLLL
                     EDAAQMYAIPLIAIEKAYQVDPRALKRVGRYWLIEKNGVTLPVFRIAELFHSDTTLEG
                     SFYLIEAAAAGGKFGLMVQKIVGRQEIVLKPLGDYLGKVTGISGTTILGNGHVALILD
                     TKTIADSVTNILRQDANAGKSAEGAA"
     assembly_gap    1562..1571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1572..>1845)
                     /locus_tag="PLY93_13835"
     CDS             complement(<1572..>1845)
                     /locus_tag="PLY93_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70605.1"
                     /translation="PTQSESARPPAAKTPTLAENNETAVTQVRRQGSMRVDLAKIEDL
                     VNLIGELIINKNQVDALSNTLVAAHEKNQTDDKAQIADFHSAKNQLS"
BASE COUNT          375 a          487 c          475 g          498 t
ORIGIN      
        1 tttgcgccgc gctatttgtt tcttctacca acatatcatc gagcgctgcg ctcgttggcg
       61 cactctgcat tgtgtcgcca cttttttcgt gcgcggtgta agaagaaagt ttcgttaaaa
      121 taccgtcgat gtcgggcaac ggtttatttt cttgaatcgc caacacttgg ctttgcaaga
      181 gggtcaatcc ctcgagcaaa atatccaaaa gttcgggggt ggtagtgacc gtttgctcgc
      241 gcaacttatc gagcatcgtt tccaactcgt gcgcgagaat gcgcaccgcg tcgacaccaa
      301 tagccgccgc gtcgcccttc accgaatgga agtggcggaa aatgtcgttg atgatgtcga
      361 tgtcgctggg gtgtttttcc aaatcgatga acgcgctctc tgcctcggag aggtgctctt
      421 cgcagccgat gaaaaattct ttgaggagtt cagaatccac cgccgctctg ttaaaactca
      481 tgctgccccc tctgcgcttt ttccagcgtt cgcgtcttgg cgcaaaatgt tcgtaaccga
      541 gtcggcaatc gtcttcgtgt ctaaaataag cgcgacgtgc ccattgccta aaatggtagt
      601 gccactgatg cctgtcactt tgcccaaata atcgcccaac ggttttagta caatctcttg
      661 gcgtcccaca attttttgta ccatgagacc aaacttaccg cctgccgccg ccgcttcgat
      721 gagatagaac gaaccttcga gcgtggtgtc actgtggaaa agttcggcga tgcgaaaaac
      781 cggcagagtc actccatttt tttcgatgag ccaataacgg cctacgcgtt tcaacgccct
      841 cggatcgact tggtacgctt tttcaatcgc gatgagcggg atggcgtaca tttgcgcggc
      901 gtcttctaaa agcaaccctt ccagaataga aagcgtgagc ggcagcttta aggtgatgcg
      961 cgtgcccacg ccttgcgccg actcgacatc gacagtgccg tttaaccgca agatattatt
     1021 tttcaccacg tccatgccga cgccacgccc tgaaagctcg gagactttgt cggcggtgct
     1081 aaagcccgct gcaaaaatga gttggaaaat ttcccgctcg ctcaaatttt ctgccgtctt
     1141 ggccgacacg atcccttttt caagcgcctt tgctaaaatt ttttgggtgt tgagaccgcc
     1201 accgtcgtct tcgacaaaaa tagagacgcg gtcgccttcg tgttcggcgc ggatgagaag
     1261 agtaccctcg cgcgattttc catttttcac gcgcacgtcg ggcatttcga tgccgtggtc
     1321 gaccgagttg cggatcatgt gcaagagcgg gtctgaaatt tcttctaaaa tagctttgtc
     1381 gagctcggtt tcttcgcctt cgataatcac gcggatttct ttgtgttgct tgcgcgccat
     1441 gtcgcgcacc gccgccgggt attttttgag cgtctgtcca atcggtacca tgcggatacc
     1501 caatgccaag tcgcgcagct tgccggcgaa atactgatcg agctcgcgcc gcgcttgatc
     1561 gnnnnnnnnn nagctcaact ggttctttgc cgaatgaaaa tcggcgattt gcgctttgtc
     1621 gtcggtttga tttttttcgt gcgcggcaac aagtgtgttg gagagtgcat cgacttggtt
     1681 tttgttgatg atgagctcgc cgatgagatt caccaagtct tcaatttttg caagatcgac
     1741 gcgcatcgaa ccttggcggc gcacttgcgt cacggcggtt tcgttatttt cagcaagcgt
     1801 gggcgttttt gccgcaggag ggcgcgcgct ttcactttga gtagg
//
LOCUS       DAOMPD010000534         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24209_length_1842_cov_2.393397, whole genome shotgun sequence.
ACCESSION   DAOMPD010000534 DAOMPD010000000
VERSION     DAOMPD010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24209_length_1842_cov_2.393397"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..>1213
                     /locus_tag="PLY93_13840"
     CDS             215..>1213
                     /locus_tag="PLY93_13840"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRP70606.1"
                     /translation="MQQLKYLFLFLVTAFAFLHCVNITDGYQGTEQLNTALSSSCSVN
                     EAYYQGIDTCLTNNAFKSELQKRLRGQRVLRYTENTEPFSAYFTKKFMTLSNVGYPVE
                     KRFDVWDAYVIFAEKAANPVTGCAQGELYDYYDGQCYITPTEIMSVSSGGDQNTYNRE
                     HSWPKSWFAAGATPVNDAAQGKYCYNGNGEGISWDTAKNWDFRAFTDLHHLIPSRDSV
                     NTSRSNYSYGVVDVNEGGFPSGNNSKFGSPKTADMPGFIAGATTTKVFEPPANLKGDI
                     ARNYFYMATRYYTEDGCWNSNEAVTRANINKWLEDLLRQWHTADPVSQGERDRNDWI"
     assembly_gap    1214..1223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1393..>1842)
                     /locus_tag="PLY93_13845"
     CDS             complement(1393..>1842)
                     /locus_tag="PLY93_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP70607.1"
                     /translation="LRSAPAVGAAVMGIILVFIPIRRHAGKILLAAVALFGVIMILFG
                     LNTHFYTAIVLLTLSGAVDNVSVVMRSTILQTHTNDQIRGRIAAINSIFIASSNEIGA
                     FESGLTARYIGLVPSVVFGGCMTVLVVVLVAWRIPSLRRLSFADIQK"
BASE COUNT          524 a          412 c          447 g          449 t
ORIGIN      
        1 aatattgcga aaggggccaa ggtgcgcatg acggggccga tttttccgcc cgaatacaac
       61 ggcgcagata cgaatctgag tttgatgttg ggattgcgtt cgcaagtgga gacgttgaag
      121 taagatgtct cggggatact ttatcttacc atcgatgatc ttcaggcaaa gtctatgact
      181 ttcctaaaaa tcaaaagaca atggtaggtg tataatgcaa cagttaaaat atctttttct
      241 tttccttgta accgccttcg catttctcca ttgtgtgaat attaccgatg gctatcaggg
      301 aacagagcaa ttgaatacag cgctttcgtc ctcgtgctcg gtcaacgaag cgtattatca
      361 aggcatcgat acatgcctca ctaataatgc gttcaagagc gagcttcaaa aacgccttcg
      421 tggccagcgg gtgttgcgct acacagaaaa caccgaacct ttttcggcgt actttactaa
      481 aaaatttatg acgctctcta acgtagggta tcccgtcgaa aaaagattcg atgtatggga
      541 tgcgtatgtc atttttgccg aaaaagcagc caatcccgtt actggttgcg ctcagggaga
      601 gctctacgat tattacgacg gtcaatgtta cataacgcct acagaaatca tgtcggtgtc
      661 gtcggggggc gatcaaaaca cgtataaccg cgaacattcg tggccgaaga gttggtttgc
      721 ggcaggagcg acaccagtga acgatgctgc acagggtaaa tactgttaca acggtaatgg
      781 cgaagggata tcttgggata ccgcaaaaaa ttgggatttt cgagcgttta ccgatttaca
      841 ccatttgata cccagtcgtg attctgtgaa cacatcacgt agtaattatt cctacggtgt
      901 tgtcgatgtg aacgaaggcg gatttccatc aggtaacaat agcaaatttg gttctcccaa
      961 aacggcggat atgcctggct ttattgcggg ggctactacc acaaaagttt ttgaaccacc
     1021 ggcaaacctt aaaggcgata ttgcgcggaa ttacttttat atggcgacac ggtattatac
     1081 tgaagatggt tgctggaatt ctaacgaagc tgtgacccgt gcaaatatca acaagtggct
     1141 tgaagatttg ctacgtcaat ggcacacggc agaccccgtt tcacaaggcg aacgtgaccg
     1201 caacgattgg attnnnnnnn nnncgcattc aagggaaccg taatccattc atcgaccacc
     1261 ccgaatgggt ggctaaaatt agcgattttt aagcctcgcg agttgaggta tggtttcgta
     1321 cggcggcgac ccaaaagagt tagaaacaat aagacgaatg ccaactatat ccgttgcgtt
     1381 tctggcatgc tcttactttt gaatatccgc aaaacttaac cgccgtaggc ttggtatccg
     1441 ccatgcgacg agcaccacca ctaaaaccgt catgcacccg ccaaagacaa ccgatggcac
     1501 gagccctatg taacgcgcag tcagcccgct ttcaaaagcg ccgatttcat ttgaagaggc
     1561 gataaagata gaattgatgg cggcaatccg gccacggatt tgatcgttgg tgtgcgtctg
     1621 tagaattgtc gagcgcatca caacgctcac attgtcaaca gcgcctgaaa gagtcagtaa
     1681 gacaatggca gtatagaagt gggtatttag accgaacaaa atcataatta caccaaagag
     1741 agcaacagca gcgagaagaa ttttccctgc atggcgacga ataggtataa aaacaaggat
     1801 tattcccatc acggcggcgc ctacggcagg tgctgagcgc ag
//
LOCUS       DAOMPD010000535         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24358_length_1832_cov_2.313450, whole genome shotgun sequence.
ACCESSION   DAOMPD010000535 DAOMPD010000000
VERSION     DAOMPD010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24358_length_1832_cov_2.313450"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLY93_13850"
     CDS             <1..1262
                     /locus_tag="PLY93_13850"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HRP70608.1"
                     /translation="VVFQKAGTTDGGIYINPTTGTTSEAGGTQTIGISLTSKPTATVM
                     LTISSSNTAEGQIKRSSGTCDTAGSVAAGSCTLTFTDADYLTPKNIAVVGQNDNVVDT
                     GTAYKLQISASSADPLYTIAQFDAASITNTDNDTAGFVATPASGLVTRPSGGGATATS
                     QIKLSSQPSANVTFSVKSDNTAGGDVTTPAGKSFTFTSANWNTPQNLVVTGGATTPVD
                     YKIIIDGTVTSTDTNYSGVTGTALHTGISLKNIAAGDKYIFFTSSASSGSFGGASGAD
                     AKCNAAANKPNTSTYKAIIGGGGRTACTTANCGTGGATEHTDWVLLPTQRYTRSDGTE
                     IGTTNTAGIFIFPLTNAVSTSSSSPWTGLSTTWLTSFSCSNWSTTTGTGTAGVTSYVS
                     SMVLDAGFGGGNGLGCSVNYPLYCAEQ"
BASE COUNT          467 a          488 c          492 g          385 t
ORIGIN      
        1 aagttgtttt tcaaaaagcg gggactacgg atggcggcat ctacatcaac ccgacgaccg
       61 gaacaacgag cgaagcgggc ggtacgcaaa ccattggcat ttcgctcacc tctaaaccca
      121 ctgcaacggt aatgctcacc atttcgagta gcaacaccgc cgaagggcag ataaagcgta
      181 gcagtggtac ttgcgatacg gcgggaagcg tcgcggcggg tagttgtact ctcacgttta
      241 ccgatgccga ttacctgaca ccgaaaaata ttgccgtcgt tgggcaaaac gacaacgtag
      301 tcgatacagg cacggcatac aaactacaaa tttctgcatc gagcgccgac cctctctaca
      361 ccattgcaca attcgacgcg gcgagcatca ccaacaccga taacgatacg gcggggtttg
      421 tcgcgacgcc agcgagcggc ctagtaacca gaccgtcggg aggcggggct actgctacaa
      481 gccagatcaa actctctagc caaccaagtg cgaatgttac ctttagcgtc aagagcgaca
      541 atactgcggg tggtgatgtg acaacgcctg ccggtaaatc attcactttt accagcgcca
      601 actggaatac accgcaaaat ctggtggtga ccgggggagc gacgacgccc gtggattata
      661 aaatcattat tgatggtacc gttacgagca cagacacgaa ctattcgggc gtaacaggca
      721 ctgcgctcca cacgggcata agcctgaaaa atattgctgc gggcgataaa tatatatttt
      781 tcacaagctc ggccagcagt ggcagtttcg gcggtgcttc tggcgccgac gcaaaatgta
      841 acgctgccgc caataaaccc aacacgagca cgtataaggc cattatcggt ggtggtgggc
      901 gcacggcatg cacaacggcc aattgtggta caggcggcgc taccgaacac accgactggg
      961 tgttgttacc cacgcaaaga tatacccgct cagacggtac agaaatcggt acaacaaaca
     1021 cagccggtat ttttatcttt ccactgacta atgccgtcag cactagtagt agctcgccat
     1081 ggacaggtct gtcaacgact tggctgacat ctttttcttg ctcgaattgg tcaaccacca
     1141 ccggaactgg tacggcgggc gtaacttctt atgtgtcttc gatggtgcta gacgcaggtt
     1201 tcggcggtgg caacggtctt ggctgcagcg tcaattaccc cctttactgc gcagagcaat
     1261 agcagaaaaa aaatcgcagg ccggattcag aagcctgacg agtcgatttc gtgagttcta
     1321 tgagactctt gatgttgcgg aatagagttg tataaacctt aatcttaccg ttccttctct
     1381 tgaaagaaat tccatcgtag aaaactctat attcattaca ctttggcgtt atgcattttt
     1441 tatttacatc agaaatttgc gccggcaagg agggcggcgg attacacagg cgcagctatg
     1501 tcagagaacg cgggatttac aagacgtttt tgcgccgcat aaaaaagtct atttctcttg
     1561 cgtagccgcg tatccaggcg caaaaatgtg cctggagcac ctgcccgacg agcgctaaac
     1621 caacctcccg gttgcgcgag gagggcatct tcggtgcgca aggccgagga cgcgtggaac
     1681 acccgggacc cggagaaggt cccgggcgcg tacacggtgg actcccggtg gcgcaatcgc
     1741 gcggagttcg tcacgggccg cgcagagatc gtggccttcc tgacccgcaa gtggacccgc
     1801 gagctggact accggctgat caaggagctg tg
//
LOCUS       DAOMPD010000536         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24408_length_1829_cov_2.904171, whole genome shotgun sequence.
ACCESSION   DAOMPD010000536 DAOMPD010000000
VERSION     DAOMPD010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24408_length_1829_cov_2.904171"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1329
                     /locus_tag="PLY93_13855"
     CDS             400..1329
                     /locus_tag="PLY93_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRP70609.1"
                     /translation="MNQLTQYFPFIAAAASLLALFEPTLFTWFNTSLIKIALAFIMFF
                     TGLSLHAADFRRVVKNPRPVVIGFILQYSVMPTIGYLAAQFFHLPKELAAGLILVACC
                     PGGTASNVISYLAKADVPLSVSMTACSTLAAVFFTPVLSSYLIGSRVPVDAWAMMFDT
                     VLVILLPIFLALLLKRTAVRIIMRLEKGGNFIAVLLITLIVASIIGSSRTQIFQGGFT
                     LVLAVLTLHTGGFFFGYILSRFFKLDLIRARTVSIEVGMQNSGLGVVLATKHLSALAA
                     VPCAISSLVHSLIGSIVAAWWSRRSHQATENSE"
     assembly_gap    1522..1567
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1568..>1829
                     /locus_tag="PLY93_13860"
     CDS             <1568..>1829
                     /locus_tag="PLY93_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRP70610.1"
                     /translation="RRKAEGAPLFLLHDGPPYANGEFHLGHGLNKVLKDIFNKYKLLR
                     GYRVPYVPGWDCHGLPIELAAIKKLGKNKDNSDKDPLRIRQAC"
BASE COUNT          462 a          393 c          402 g          526 t
ORIGIN      
        1 gtaaactaat cgatgtcttt aacatatttg cctctatata ttaaatacca gaaaatggtc
       61 aaattttctc acttttataa aaaaaagtac aatttttgat aaaaattata ttttttttgc
      121 agaaatcatt cgtgtggata gttataccct aatgtgacat aatatcaaaa actgggatag
      181 ttatccatcg atgtgacata atactggaac caagacttcg aaatttaaac ctgaataaaa
      241 gccccagaat tggtatgaag ttaaggagca aaaaaactaa tacgtattca gactaaccat
      301 ctaattatcc gtcttaggaa gaaaattcat cttgttgcat actaccaggc tccatggcgc
      361 ttgacagcgt agcggattaa gcagtgatta gacatagccg tgaaccagct cacccaatac
      421 ttccccttta tcgccgcagc agcttctcta ctcgcgttat ttgagcccac actctttacg
      481 tggttcaata catcgcttat taaaattgct cttgcgttca tcatgttttt tacggggcta
      541 agcttgcacg ccgccgattt tcgtcgtgta gtaaaaaatc cacgccccgt tgtgattgga
      601 tttattctgc agtacagtgt gatgcccaca ataggctatc ttgcagccca attttttcat
      661 ttgccgaaag agttggcggc agggctcatt cttgttgcgt gttgcccagg gggtacggcg
      721 tctaatgtta ttagctatct tgcgaaagcg gatgttccat tgtcggtgag catgactgcg
      781 tgttcgacgc ttgcggcggt attttttacg cctgtgcttt cgtcgtacct cattggtagc
      841 cgtgtgcctg tggacgcgtg ggcgatgatg ttcgacacgg tattagtgat cttgttgccg
      901 atttttctcg cgttgctttt gaaacgcacc gctgtacgaa taattatgcg tcttgaaaaa
      961 ggcggtaatt ttatcgccgt acttcttatc accctcatcg tggcgtcgat tatcggtagc
     1021 tcgcgcaccc aaatctttca aggaggattt actcttgtcc ttgctgttct tacactgcac
     1081 accggcggct ttttctttgg gtatatttta tcacgcttct tcaagcttga ccttatccgt
     1141 gcgcgcaccg tttcgattga agtgggaatg cagaactcgg gcttgggggt tgtactggca
     1201 actaagcatt tgagtgcgct tgccgcagta ccgtgcgcaa tttcttcttt ggtacatagt
     1261 ctcatcggga gcattgtggc agcgtggtgg tcgcggcgta gccatcaagc gactgagaac
     1321 tctgagtaga tacgctgtgc agcctgtctt cactttgaat tatttccgta agagaagtaa
     1381 tcccacttat gagccaaaat aaagatgaaa ataaacaatt aaaagaaaag aacccgtggg
     1441 agcacacgat atgtctgccg cagacggtgt atccgatgcg cgctgattta tcgaagcgcg
     1501 aaaatgaaat tatcgactat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnacg ccgcaaggcc gagggggcgc cgcttttttt attacacgat ggcccaccgt
     1621 acgcaaacgg cgagtttcat ttggggcatg gactcaacaa agttctcaaa gatattttta
     1681 ataagtataa gctcttgcgt ggctatcgcg tgccgtatgt ccctggttgg gattgccacg
     1741 gattaccaat tgagctggcg gccataaaaa aattgggaaa aaataaagac aattcagaca
     1801 aagacccact gcgtatccgc caagcctgc
//
LOCUS       DAOMPD010000537         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24447_length_1827_cov_2.451467, whole genome shotgun sequence.
ACCESSION   DAOMPD010000537 DAOMPD010000000
VERSION     DAOMPD010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24447_length_1827_cov_2.451467"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLY93_13865"
     CDS             complement(<1..671)
                     /locus_tag="PLY93_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70611.1"
                     /translation="MKSLVALLKKTAGLKGWQVSRLSKNSTEVYYVGRELETVRCNAV
                     SNYTLRVQTPCEDNFMGESATRFQGKPVSLNALVEETKERAELVKNPAYRFAESSPLL
                     SAIHPPIDDIQIRNADLDTLIAVGVKAQEYRQKHLARFPLNSLELFFEEYTLDIQNHR
                     GLICHTPSTRLVCDYVLTSPDNAHEIMGIKKRRFLSDLCIEEEWENDARILSDLTQAT
                     LPPTG"
     gene            complement(675..1043)
                     /locus_tag="PLY93_13870"
     CDS             complement(675..1043)
                     /locus_tag="PLY93_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70612.1"
                     /translation="MRRLIVIALIAPLIWGCTGLVDDEKKGEICIHYRQQLTYPNSSC
                     TEGAETDTKQAYFCEDDVKEINCLTLPTTSCPASAPYITYTDNAKYYGKRKCADDGYI
                     TSCPSDAKYKVADNTAFCPN"
     gene            complement(1088..>1827)
                     /locus_tag="PLY93_13875"
     CDS             complement(1088..>1827)
                     /locus_tag="PLY93_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRP70613.1"
                     /translation="GIVEEFKNVKQIKLMKLEAVDEATDLSRFWARTPHSRRQIYTQI
                     RHLIQKIASNELREVCLHFLKNRAFMRLFLEAPASRNVHHAYIGGLMEHTLNVMQTTA
                     AFCRVYPHADIDLAIAGAFLHDIGKVDEYDFLLSHYEHSEAGRLKGHTLLSYERLKPV
                     ITHINNMHPITQAKLEHVILAHQGKRIWGAIEEPRFLEAYLVHAADAVDSTQFIFHDL
                     KRTAEERGGESAWSEYSRELGREVFLR"
BASE COUNT          438 a          419 c          460 g          510 t
ORIGIN      
        1 tttcccgtcg gcggtaaagt tgcttgcgtc aaatcggaca aaatacgcgc gtcgttttcc
       61 cattcttctt cgatacacaa gtcgctcaaa aaacggcgtt ttttaatccc cataatttca
      121 tgcgcattgt cggggctcgt gagaacatag tcgcacacaa ggcgtgtcga gggcgtatga
      181 caaatgagcc ctcgatggtt ttgaatatcg agcgtgtatt cttcaaaaaa aagttccaaa
      241 ctgtttaagg gaaagcgggc caagtgtttt tggcggtact cctgcgcctt tacacccact
      301 gcgatgagcg tatctaaatc ggcgttgcgt atttggatgt cgtcgatggg aggatggatt
      361 gctgaaagaa gcggagagct ttctgcaaaa cgatacgcag gatttttgac gagctctgcg
      421 cgttctttgg tttcttccac gagcgcgttg agagaaaccg gctttccttg aaaacgagtg
      481 gcgctttcgc ccataaaatt gtcttcgcac ggagtttgta ctcgaagcgt atagttactc
      541 actgcattgc agcgcaccgt ttcgagctcg cggcctacat aatacacctc ggtggagttt
      601 ttggaaagac ggctcacctg ccaaccttta aggccggcag tttttttgag taacgctacg
      661 aggcttttca agttttagtt agggcagaaa gcggtattgt cggcgacttt atattttgca
      721 tccgatgggc aagaggttat gtagccatcg tcagcacatt ttctcttccc gtaatatttt
      781 gcattatcgg tataggtgat atacggcgct gatgcaggac aagatgttgt gggtaatgtt
      841 aagcaattaa tttctttcac atcgtcttcg cagaaatacg cttgcttagt atccgtttca
      901 gcgccttcag tgcatgatga attaggataa gtaagttgtt gccggtagtg aatacaaatt
      961 tcgccctttt tttcatcgtc cacaagtccg gtacaccccc aaattagagg tgcaatcaaa
     1021 gcgataacaa tcaagcgacg cataccgtcg catagcagag cctatgcgcg gtgtcaagaa
     1081 tttgcgctca tcgtaaaaat acctcacgcc ccagttcgcg gctatactcc gaccacgcgc
     1141 tctcgccgcc gcgttcttca gcggtgcgtt ttaagtcgtg aaaaataaat tgcgtcgagt
     1201 cgaccgcatc ggcggcgtgg actaaatacg cttccaaaaa acgcggttct tcaatcgccc
     1261 cccaaatcct tttgccttgg tgcgccaaga tgacgtgttc aagctttgct tgggtgatag
     1321 ggtgcatatt attaatgtgg gttatcaccg gctttaatcg ttcataacta agtagagtgt
     1381 gtcctttgag tctccctgct tcggagtgtt cgtaatggct aagtaaaaaa tcgtactcgt
     1441 caactttgcc gatgtcgtgc aagaaagcgc cagcaatggc taaatcgata tcggcgtgcg
     1501 gatagacacg gcaaaatgcg gcggtcgtct gcatcacgtt gagcgtgtgc tccatgaggc
     1561 cgccgatata cgcgtggtgg acgttacgcg acgcaggggc ttcaagaaag aggcgcatga
     1621 atgcacggtt tttcaaaaaa tgcaaacaca cttcgcgcaa ttcattcgag gcaatttttt
     1681 ggatgagatg gcgaatttgg gtataaattt gccgccgtga atgcggtgta cgcgcccaaa
     1741 agcggctgag atcggttgct tcgtcgaccg cctcgagttt catgagtttg atttgtttta
     1801 cattcttgaa ttcttcgacg atgcctt
//
LOCUS       DAOMPD010000538         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24551_length_1819_cov_2.751701, whole genome shotgun sequence.
ACCESSION   DAOMPD010000538 DAOMPD010000000
VERSION     DAOMPD010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24551_length_1819_cov_2.751701"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLY93_13880"
     CDS             complement(<1..207)
                     /locus_tag="PLY93_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRP70614.1"
                     /translation="MQHKVDIGIIGGTGVYGVEGMQILDRIKIDTPWGHPSDAITIAS
                     YGEGASAITLAFLPRHGKGHFIPPT"
     gene            complement(239..1729)
                     /locus_tag="PLY93_13885"
     CDS             complement(239..1729)
                     /locus_tag="PLY93_13885"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRP70615.1"
                     /translation="MPQTKKKNSPKSVNPINSFLAYPKKIQCEATGSTDPKRPQVCFV
                     TKEELPSLKEIPPFFKAEPNEVLAVKEGFIVGLGEIKKLDADKFADTALAALQTAATK
                     LSSYDILLSQENIEKVGFPTVLRLLAVAQANAEYPTDLLKSAKNQKRVLVKNTRIVVP
                     DSFLPIAKGVLASALVEARHICAMRQTQALPGNYLTPDTMKERATDIAKMFKLSIKSF
                     GFNDLVKMGAGGILAVTIGSAREPQLIVLEYKHPKAKKTLSLVGKGVTFDTGGISLKP
                     GGDMHEMKFDMSGSAMALHAIASIAEQKLPVNVVCAIAMVENMPSGTAFKPGDVYTAL
                     NGQTVEVQNTDAEGRLILGDTLTYVQKHYKSDILIDMATLTGACVVALGDFYAGLFSN
                     NVGARSIVEKAAAVSHEPVWPLPVGPRYRKMLKSDIADINNVGGRWGGASTAAAFLES
                     FIEEGQAWAHLDIAGVGMVKKPFNVYTPAGSGYGVRLLTEIARQLA"
BASE COUNT          415 a          494 c          427 g          483 t
ORIGIN      
        1 tgtcggcggt ataaaatgcc ccttgccgtg gcgcggtaga aacgccaacg tgatggcgct
       61 tgccccttcg ccatacgaag cgatcgtgat ggcatccgaa gggtgtcccc acggtgtatc
      121 gattttaatt ctgtcaagga tctgcatccc ttcgacgcca taaacgccag tgccgccgat
      181 aatcccgata tctactttat gctgcatttc tctctctttc tattcgttaa taacaacact
      241 acgccaactg acgcgctatc tcggtgagca aacgtacacc gtaaccgctg cctgcgggtg
      301 tgtacacatt aaatggtttc ttcaccatcc ccacaccggc gatgtcgagg tgtgcccacg
      361 cttggccttc ttcgataaac gactctaaga acgccgctgc cgtcgacgct cccccccaac
      421 ggccaccgac gttgttgatg tcggcgatgt cggattttag cattttgcgg tagcgtggcc
      481 ccacgggtaa cggccacacc ggttcgtgtg aaaccgccgc tgctttttcg acaatgctac
      541 gagcgcctac attattggaa aaaagtcctg cgtaaaaatc tcccagcgcc actacacacg
      601 caccggtgag cgttgccatg tcgatgagaa tatcgctctt gtagtgtttt tgtacatacg
      661 tgagagtatc gcccaaaatg agacgcccct cggcgtcggt attttgtacc tccaccgttt
      721 gtccgttgag cgcggtatag acatcgcccg gcttgaaggc agtccccgat ggcatatttt
      781 ctaccatcgc aatcgcgcac accacattca ccggtaattt ctgttcggcg atggaagcga
      841 tcgcgtgcag cgccatcgcg ctccccgaca tatcgaattt catttcgtgc atatctccac
      901 cgggcttgag tgaaatacca ccggtgtcga aagtcacccc tttacccacg agcgacaatg
      961 tcttttttgc cttggggtgc ttatactcga gcacaatgag ttgcggttcg cgcgccgaac
     1021 caatggtgac agcgaggatc ccccctgcgc ccatttttac taagtcgtta aaaccaaacg
     1081 attttatgga gagcttaaac attttcgcaa tgtcggtggc gcgctccttc atcgtgtcgg
     1141 gcgttaaata attcccgggg agcgcttgcg tctgccgcat cgcgcatata tgccgcgctt
     1201 caacgagcgc cgaggcaagc acgcctttgg caattggcag aaacgaatcc ggcacaacaa
     1261 tgcgtgtgtt cttaactaaa actcttttct gattttttgc cgacttcaat aaatcggtgg
     1321 gatactctgc attcgcttga gccaccgcca ataaacgcaa caccgtgggg aatcccactt
     1381 tttcgatatt ttcttgcgac aacaaaatat cgtagcttga aagtttagtg gccgccgtct
     1441 gtagcgctgc caatgccgtg tcggcaaatt tatcggcgtc gagttttttt atttcgccca
     1501 accccacgat aaacccttct ttaacggcca acacctcgtt aggctcggct ttgaaaaaag
     1561 gtggaatttc tttcagagac ggcaactctt ctttagtgac aaaacacacc tgtggtcgtt
     1621 tgggatcggt agaccccgtg gcttcgcatt gaattttttt ggggtacgct aaaaaggagt
     1681 tgatgggatt tacacttttg ggggaatttt tcttttttgt ttgaggcata agcttgcccc
     1741 ttgtcttaaa ctcaattagc ccgaaaagga aaaagaggtt atgcgcattt ataaattaca
     1801 ttcgataaaa ccggcgaca
//
LOCUS       DAOMPD010000539         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24570_length_1818_cov_2.454906, whole genome shotgun sequence.
ACCESSION   DAOMPD010000539 DAOMPD010000000
VERSION     DAOMPD010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24570_length_1818_cov_2.454906"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLY93_13890"
     CDS             complement(<1..348)
                     /locus_tag="PLY93_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRP70616.1"
                     /translation="MSEFHIEGGHKIGGSITPQGNKNEALPVLCAALMNTHEVIIRNV
                     PQIEDIKHLLKIMEAIGASIATLDDGHSYKIRTPEIWLSELPIELAAGIRGSVTLLGP
                     LLSRAKKVFLPRPG"
     gene            complement(526..1338)
                     /gene="dapB"
                     /locus_tag="PLY93_13895"
     CDS             complement(526..1338)
                     /gene="dapB"
                     /locus_tag="PLY93_13895"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRP70617.1"
                     /translation="MIGFGIVGSLGRMGRAIAKQAASNTTDAKLSFQLTAPVEHAAHA
                     DLGKKYNAVTGIAFDATITTLDKALIPQGGFIDFSHASTVIHTLQKAAEKGVPVVVGT
                     TGFTKEERAAIESMAVKIPLVIASNMAIGVNVLFSLVGEAARSLAPHGFDPEVMEIHH
                     NLKKDAPSGTATTLKEEILRAYNRDEESVLYGRHGMIGARKENEVGVMALRGGDVVGD
                     HTVYFLGQGERIEIKHQAVSRDTFASGALTALRFLQGKKPGLYSMKDVLGLK"
     gene            complement(1340..>1818)
                     /locus_tag="PLY93_13900"
     CDS             complement(1340..>1818)
                     /locus_tag="PLY93_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRP70618.1"
                     /translation="NIPGRSAVGLTLDTIKELSMHGNIPAIKEATGDLGFMAQIIEAT
                     EGRMTLLSGDDNLIMPVLSIGGQGVVSVIANVYPQETGAITRDYFANKVEEARKKFLK
                     LLPLCKAMFIETNPIPVKYAASQKGLCVNSLRLPMTPLGSSNEAALNAAMQTFERG"
BASE COUNT          429 a          465 c          438 g          486 t
ORIGIN      
        1 cccgggtcgc ggcaaaaaaa ctttcttcgc acgcgataaa agcgggccca aaagagtcac
       61 cgaaccacgg atacccgcag caagttcgat gggaagctcg ctcaaccaaa tttctggagt
      121 acggatttta tacgaatggc cgtcgtcgag cgtggcgatc gacgctccga tagcttccat
      181 aattttaagc aaatgtttaa tgtcttcaat ttgcggaaca ttacggatga tgacctcgtg
      241 cgtgttcatg agcgctgcac acagcacagg cagcgcttcg tttttattcc cttgaggggt
      301 gatgctgccg ccgattttat ggccgccttc gatgtggaat tctgacatgg tttttaatgc
      361 gtcgcaacgc ggcgtagaat acgttttttt tgagtgcgtg taaatcgtta ttccatttct
      421 taaagcggca taaggctcga taaggagcct gtgcgccagt tgaaataaag accgagaatt
      481 ttttgagcac gttgtgtgcg agcgtggttt aaaatggtcg tattgttatt tcaagcctaa
      541 aacatctttc attgaatata agccgggttt tttaccttgg agaaaacgca gagcggtcaa
      601 cgcacccgaa gcaaacgtgt cgcggctcac cgcttgatgc ttaatttcga tacgttcgcc
      661 ttggcctaaa aagtaaacgg tgtggtcacc cacaacgtcg ccgccacgca gcgccatcac
      721 acccacttcg ttttctttac gtgcgccaat catgccgtgg cgtccataaa gcacactttc
      781 ttcgtcgcgg ttatacgcgc gtaaaatttc ttctttgagc gtcgttgccg tacccgacgg
      841 agcgtctttt ttaaggttgt gatggatctc catcacctcg ggatcaaagc catgtggagc
      901 caacgagcgc gccgcctcgc ccaccagtga gaagagcacg ttcacgccaa tcgccatatt
      961 cgatgcaatc acgagcggga ttttcaccgc catgctttca atcgcagcac gttcttcttt
     1021 cgtaaaacca gtggtgccca caaccacagg tacccctttt tcggcggctt tctgcaatgt
     1081 atggatgacg gtcgatgcat gcgaaaaatc gatgaatccc ccttgcggta tgagcgcttt
     1141 atctaaagtg gtgatagtgg catcaaacgc aatgccagta actgcgttat atttttttcc
     1201 caagtctgcg tgtgcagcgt gttccactgg agcggtgagt tggaatgaaa gttttgcatc
     1261 ggtggtgttc gatgccgctt gctttgcaat cgcgcgtccc atgcgcccta acgagccgac
     1321 aataccaaat ccaatcatac tatcctcttt caaaagtttg catcgctgca ttgagcgccg
     1381 cttcgttgga agaacccaac ggggtcatgg gcaagcggag cgaattgacg cacaacccct
     1441 tttgagacgc ggcgtatttc accggtatgg gatttgtttc gataaacatc gctttgcaaa
     1501 gcggcaagag tttcaaaaac tttttccgcg cttcttctac tttgttcgca aaataatcgc
     1561 gcgtgatggc gccggtttct tgtggataca cattggcaat cacgctcacc acaccttgcc
     1621 cgccgataga caacaccggc ataattaaat tatcgtctcc ggacaagaga gtcatacgcc
     1681 cttcagtggc ctcaataatt tgtgccataa atcccaagtc ccctgtggct tctttgattg
     1741 ccggaatatt cccatgcatt gaaagttctt taatcgtgtc gagggtaagt cccactgccg
     1801 aacggccagg gatgttgt
//
LOCUS       DAOMPD010000540         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24652_length_1814_cov_2.611143, whole genome shotgun sequence.
ACCESSION   DAOMPD010000540 DAOMPD010000000
VERSION     DAOMPD010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24652_length_1814_cov_2.611143"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1392
                     /gene="der"
                     /locus_tag="PLY93_13905"
     CDS             52..1392
                     /gene="der"
                     /locus_tag="PLY93_13905"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRP70619.1"
                     /translation="MPAIAIIGRQNVGKSSLFNSLIGARKAIVYNSPGVTRDLLEEKV
                     PWGEGAWTLTDFPGFEDAKLLRNDALTQAAITAAQKKLDKFHLLLFVVARGGLTSFET
                     DLLNRLRRHKKPVWLVVNFIDDPQLEADAAEFHELGFSQTFFVSALNRRNVDFLKENI
                     QIHFQSGKKESIGNVVNPTLENENADLKLAILGKPNAGKSTLFNTFTGKERALISPIA
                     GTTRDSIDEIFHFQGKRIKIVDTAGLRRQRNVVEDVESISVKRTKEALASADVCFLVM
                     DYQENLDKQNKTLFALIAEAGKPLIALINKLDVFRSDSETKAQIAADIAAAQKAFWNF
                     PWFFVSAESGENCIKALEKAFALKEQIFEKISTPKLNTILKELGRSHILHNQNIRLKY
                     ITQGTPLNRFILFTNKPVNAPVKRHLASSLAKKLDMTEVPVLLQVRVQESKKKK"
     gene            1435..>1814
                     /locus_tag="PLY93_13910"
     CDS             1435..>1814
                     /locus_tag="PLY93_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70620.1"
                     /translation="MQKPVALITGASAGIGFEMAKVFAARGYDLIIVARTAKKLKEFH
                     KQISPLITVTDIAMDLTSPNAAARLFTVTQKKKLKVDVLVNNAGFGSNGHFVELDRKN
                     ELQEIDLNVRVLTELCHFYGKEMA"
BASE COUNT          516 a          444 c          415 g          439 t
ORIGIN      
        1 taattatctt ctttagcgtg acattcctgg aagaatacgc tacattttct tatgcccgcc
       61 atcgccatta tcggtcgcca aaatgtaggt aagagctcgc tcttcaatag cctcatcggc
      121 gcacgcaaag cgattgtcta taattcgccg ggggttacgc gcgatcttct tgaagagaag
      181 gttccgtggg gcgaaggcgc atggacgctg accgattttc ctgggtttga agatgcaaaa
      241 ctcttacgca acgatgcgct cacacaagcg gcaatcacgg cagcacaaaa aaaactggat
      301 aaattccact tgctgctttt tgtcgttgca cgagggggat taacctcgtt tgaaaccgac
      361 ttactgaacc gccttcggcg gcataaaaaa ccagtttggt tggtggtgaa ttttatcgac
      421 gatccccaac tcgaagccga cgccgccgaa tttcatgaac tgggtttttc tcaaactttt
      481 tttgtttcgg cgctcaatcg gcgcaacgtc gattttctca aagagaatat tcagatccat
      541 tttcaatcgg gcaaaaaaga atctataggg aatgtggtaa atcccacttt agaaaacgaa
      601 aacgccgatt tgaaactggc aattttgggt aaacctaacg caggcaagag cacgctcttc
      661 aacactttca ccggcaaaga gcgcgcgctc atctcaccga ttgctggcac cacacgcgac
      721 agtatcgacg aaatttttca ctttcaagga aagcggatta aaattgtcga tacagcgggt
      781 ttgcgccgcc agcgcaacgt cgttgaggat gtggaatcga ttagtgtaaa gcgcacgaaa
      841 gaagcgctgg cgtcggcgga tgtgtgcttt ttggtcatgg actaccaaga gaatttggat
      901 aagcaaaaca aaactctttt tgcgctcatt gccgaagcag gtaaaccgct cattgcgctc
      961 atcaacaagc tcgatgtctt ccgtagcgat agcgaaacga aggcgcaaat tgccgctgac
     1021 atcgccgccg cgcaaaaagc gttttggaat tttccgtggt tttttgtgag cgccgagtcg
     1081 ggtgaaaatt gcatcaaggc gttggaaaaa gcatttgctc tcaaagaaca aatttttgaa
     1141 aaaattagta ctcccaaact caataccatt ttgaaagaac ttgggcgtag tcacattctg
     1201 cacaaccaaa acatccgctt gaaatacatc actcaaggca ctccactcaa ccgatttatt
     1261 ctcttcacca acaaacccgt caacgcaccg gtgaagcggc acttggcctc gtcactcgcc
     1321 aagaagctcg acatgacaga ggtgccggtg ctgttgcagg tgcgcgtaca agaaagcaaa
     1381 aagaaaaagt agcatttttc tatacaaggc gtctaaaaaa ccttacccgc actcatgcaa
     1441 aaacctgttg cactcattac tggcgcgtcg gcgggtattg gttttgaaat ggccaaagtt
     1501 tttgctgcgc ggggatacga tctcatcatt gttgcgcgca ccgcaaaaaa gctcaaagag
     1561 ttccataaac aaatctcgcc gctcatcacc gtcaccgaca tcgcgatgga tttaacgtcc
     1621 ccaaacgcag cggcgcgcct ttttacagtg actcaaaaaa agaaactcaa ggtggatgtc
     1681 ctcgtcaaca atgcaggctt tggtagcaac gggcatttcg tcgaactcga ccgcaaaaat
     1741 gaattgcaag aaatcgattt aaacgtgcgc gttctcaccg agctttgcca tttttatggc
     1801 aaagaaatgg caaa
//
LOCUS       DAOMPD010000541         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24761_length_1807_cov_2.555936, whole genome shotgun sequence.
ACCESSION   DAOMPD010000541 DAOMPD010000000
VERSION     DAOMPD010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24761_length_1807_cov_2.555936"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLY93_13915"
     CDS             <1..342
                     /locus_tag="PLY93_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70621.1"
                     /translation="MGLAVLKCYVVDIWLWDKSVRIIAGVILGGGLVVISFYYEKFRE
                     KFKDLAAILFVVGVFSLSSPAEAAAKATATAAPPVIADTALSWPLYAAFWVIFAVIVW
                     RMFFIFRKDKV"
     gene            395..1492
                     /locus_tag="PLY93_13920"
     CDS             395..1492
                     /locus_tag="PLY93_13920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRP70622.1"
                     /translation="MQEAAAAHVGDTFIEHYERKIHSQKQLIELSKALNSTLDLRSLI
                     DSILNVSLAQSRCLQAGIYLIPEVDAHSLVLNENCIGFEIADTSEFVMDYKSDLIKLL
                     SEYPTLLTLTEIKNIAQATEHSSLAQVNYHDAILQLSRLGENLLLIPLKAKGRVNGIL
                     VMGPKSDGEEYIAEEKAFLSDLASLAAIAVENARLYELATVDMMTKLKIHHFQTRLRE
                     EMEHARETRTPLSLLLTDIDHFKKFNDTYGHQVGDIVLKEVAKVLITSAHGKDIAARY
                     GGEEFTIIAPGRSLDEMQELAEKIRERVQKMSVPNPTDKGDEVLKVTISIGVTTLRHD
                     EDHENKQIIERADQALYTAKHGGRNRVEVLK"
     gene            complement(1532..1753)
                     /gene="rpmB"
                     /locus_tag="PLY93_13925"
     CDS             complement(1532..1753)
                     /gene="rpmB"
                     /locus_tag="PLY93_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRP70623.1"
                     /translation="MARTCQLTGKGTRFGNNVSHSHRKTRRQFKANVQKKRIFLPEEG
                     RFITLNISTRALRTLQKKGLAAMMKDAGK"
BASE COUNT          492 a          412 c          417 g          486 t
ORIGIN      
        1 atggggcttg cagtcctcaa gtgttacgtg gtcgatattt ggctttggga taaatcggtg
       61 cggattatcg caggtgtgat tttaggcggc ggtttggtgg tcatcagctt ttactacgaa
      121 aaatttcgag aaaaattcaa agacttagcg gcgattcttt ttgtcgtcgg cgtattttca
      181 ctttcttcac ccgccgaagc ggctgcaaaa gctaccgcta ccgccgcgcc gccggtgata
      241 gcagataccg cactctcgtg gccgctctac gccgcttttt gggtcatttt tgctgttatc
      301 gtttggcgca tgttttttat ctttcgtaaa gacaaagtat aaaaaatgtt tattttttgc
      361 ttgttgcttt agagttactt taatttaagt gcgtgtgcaa gaagcggcag ccgcccatgt
      421 gggtgatacc tttatcgaac actatgaacg caaaattcat agtcaaaagc agctcatcga
      481 gctttcaaaa gcgctcaatt ccactctcga tttacgctcg ctgatcgact caattctcaa
      541 tgtatccctt gcgcagagtc gatgcttaca agcgggcatc tatctcatac cggaggtgga
      601 tgcccatagc ctcgtcttaa atgaaaactg tattgggttt gaaattgcag acacctctga
      661 atttgtaatg gattacaaaa gcgatttaat taaactactt tcagaatacc caacactttt
      721 gactttaacg gaaattaaaa atattgccca agcgacagaa cactcgagcc ttgcacaagt
      781 gaactaccac gatgcgatat tgcagctctc gcgcctcggt gaaaatctat tgttgattcc
      841 gcttaaagca aaaggccgtg tgaacggtat tttagtcatg gggccaaaat ccgacggtga
      901 agaatatatt gccgaagaaa aagcgttcct ctctgattta gcgtcactgg ccgcgattgc
      961 ggttgaaaac gcaaggcttt acgaacttgc cacggtggat atgatgacaa aactaaaaat
     1021 ccaccatttt caaacacgcc tgcgcgaaga aatggaacac gcgcgtgaaa cgcgtacccc
     1081 gttgagtctt ttgctcaccg acatcgacca ctttaaaaaa tttaacgaca cgtacggcca
     1141 tcaagtgggc gacatcgttc tcaaagaagt agcgaaggta ctcatcacta gcgcgcacgg
     1201 taaagacatt gcggcgcgtt atggggggga agaatttacc atcatcgctc ctggccgttc
     1261 gttggacgaa atgcaggaac ttgccgaaaa aattcgtgag cgggtgcaaa aaatgagcgt
     1321 acccaacccc actgacaagg gggatgaagt cctcaaagtt acaatttcta ttggagtcac
     1381 aacgctacgc cacgacgaag accacgaaaa caaacagatt atcgaacgtg ccgaccaagc
     1441 gctttacaca gcaaaacacg gtggccgcaa ccgagtcgaa gtcttaaaat agaaccttac
     1501 aacctttggc tatttagtgg attgaaaaag cttattttcc cgcgtctttc atcattgcag
     1561 ctaaaccctt cttttgaagg gtacgcagcg cacgggtaga aatgttgagc gtaatgaaac
     1621 ggccttcttc aggtagaaag atgcgttttt tttgcacatt ggctttgaac tgacggcgtg
     1681 tcttacggtg cgagtgactc acgttattgc cgaagcgggt tccttttcct gttaattgac
     1741 acgttcttgc cataatagtc tccagtgcgt tgtttgagaa agtcaaagtg gcgtaactat
     1801 tcagcct
//
LOCUS       DAOMPD010000542         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24773_length_1806_cov_3.297544, whole genome shotgun sequence.
ACCESSION   DAOMPD010000542 DAOMPD010000000
VERSION     DAOMPD010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24773_length_1806_cov_3.297544"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLY93_13930"
     CDS             complement(<1..334)
                     /locus_tag="PLY93_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP70624.1"
                     /translation="MAQKRISVMMVDDSALIRRALTQILSEDEGIEVLGAFSDPFLAA
                     NAMKKRLPDVVILDVEMPGMDGLTFLQKIMTQRPLPVIICSSLVTQGSDTLAKALSLG
                     AGEVIQKPK"
     gene            complement(336..1190)
                     /locus_tag="PLY93_13935"
     CDS             complement(336..1190)
                     /locus_tag="PLY93_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRP70625.1"
                     /translation="MVQVAYKTPSLSDAVWARLAPIIESEVGIQMPIDKKTLLQNRLL
                     RRLRSLDFETFEEYCDYLFSEKGQRHEMTLFLNEITTNKTYFFREFRHFEVLNDRIIP
                     EFVEKRKNSAQSFRVWSAASSTGEEVYSIACIIEEYIRSENIVLPYEILGSDISTRVL
                     WHAYDAIYDEEQVRMISPDLKRRYFLENKNPSRTQLRVAPEVRAHIKFRKINLMNPRY
                     PHLEQFDVVFCRNVLIYFSRVDIECIVEKMMAHIKPGGYFITGHADAIPIAGVGLKRI
                     EPSVYVKL"
     gene            complement(1222..>1806)
                     /locus_tag="PLY93_13940"
     CDS             complement(1222..>1806)
                     /locus_tag="PLY93_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70626.1"
                     /translation="KSQAAAAEISTLSESSVSVAKTAGDLITKLIPDIQKTAELVSEI
                     NSASAEQTNGANQISSAVEEFGSVIQENASSSEELASTSEELTAQADHLRDIVTEIIT
                     GSIEEEYERMSPVKSKKNRQGTKRTPHSISHVLEEDTFEKRPAPKVTSTFTTKHTSKG
                     VPAQSKATNHQDGFEYEMSNARDDADHEFQRIGR"
BASE COUNT          431 a          434 c          403 g          538 t
ORIGIN      
        1 ctttgggttt ttggatcact tcgcccgcgc ccaagctcaa cgccttggcg agcgtgtcag
       61 agccttgggt gacaagactg gaacagatga tcaccggcag cggtcgctgt gtcataattt
      121 tttgtaaaaa cgtcaacccg tccatacccg gcatttcgac atcgagaatc acgacatcgg
      181 gcaggcgttt tttcattgca ttggccgcca agaaagggtc gctaaatgct cccaagactt
      241 caatcccctc gtcctcggag aggatttgtg tcaacgcacg acggataagc gccgaatcgt
      301 cgaccatcat tacagaaata cgtttttgtg ccatgctata gttttacata aacgctcggt
      361 tcgatgcgtt taagccccac gcccgctatc ggtattgcgt cggcgtggcc agtaataaaa
      421 taaccgccag gttttatatg cgccatcatt ttttcgacaa tacattcaat gtctacgcgt
      481 gagaaataaa tcaacacgtt gcggcaaaag acaacgtcaa attgctctaa gtgaggatag
      541 cgtggattca ttaaatttat tttacgaaac ttgatatgcg cgcgtacctc tggggcaacg
      601 cgtagttgag ttcggctggg atttttattt tctaaaaaat accgccgctt caaatcgggg
      661 gaaatcatac gtacttgttc ttcgtcgtaa atcgcatcgt acgcatgcca taacacccgc
      721 gttgaaatat ccgaacccaa aatttcatac ggcaacacga tattttcaga gcggatatat
      781 tcttcaatga tgcacgcaat cgagtatact tcttcgccgg ttgaagacgc ggcactccat
      841 actcgaaacg attgcgcgct attctttctt ttttccacga attctgggat gatgcggtcg
      901 ttcaacactt caaagtgacg gaactcgcgg aaaaaataag ttttgttcgt ggtgatctcg
      961 tttaagaaga gcgtcatttc atggcgttgg cctttttcgc tgaaaagata atcgcaatac
     1021 tcttcaaatg tttcaaaatc caaacttctt aaacgcctca acaaacgatt ctgcaacagt
     1081 gtttttttat cgatgggcat ttggataccc acttcgcttt caatgatggg cgctaaacgt
     1141 gcccaaacgg catcggaaag tgagggtgtt ttgtatgcta cctgtaccat tatatttact
     1201 cactcctttt ttgtttaact gttatctgcc tatccgttga aattcatgat cggcgtcgtc
     1261 acgcgcattc gacatttcgt attcaaaacc gtcttggtgg ttggtcgctt tcgattgcgc
     1321 cggcacgcct ttcgaggtat gctttgtagt aaaggttgac gtcactttag gcgctggtct
     1381 tttttcaaaa gtatcttctt ccaaaacatg gctaatcgaa tgcggagtgc gttttgtccc
     1441 ttgtcgattt tttttcgatt ttacggggga catcctctcg tactcttctt ctatactacc
     1501 ggtgatgatt tcagtgacga tgtcgcgcaa atgatcggcc tgtgcggtga gttcctcaga
     1561 ggtcgacgcc aattcttctg acgacgaagc attttcttga atcaccgaac cgaactcttc
     1621 taccgccgac gagatttggt tcgcgccgtt ggtttgctct gcactcgccg aattgatttc
     1681 agagacgagc tccgccgttt tttgaatgtc ggggattaat tttgtaatca aatcgcccgc
     1741 cgttttggca acagaaacgc tcgactcgga aagcgtactg atttccgctg ctgccgcttg
     1801 actctt
//
LOCUS       DAOMPD010000543         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24873_length_1801_cov_2.022337, whole genome shotgun sequence.
ACCESSION   DAOMPD010000543 DAOMPD010000000
VERSION     DAOMPD010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24873_length_1801_cov_2.022337"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLY93_13945"
     CDS             <1..406
                     /locus_tag="PLY93_13945"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HRP70627.1"
                     /translation="LIVLVHKVGLKQYAESGVQINGLVSKEFLVSIFYGKNPLHDGAV
                     ILEGQNVVAAACYLPMSTSRDVKRAHGARHRAALGLSEDSDALVIVISETSGKVTLAF
                     LGEMKENIDPARLRKILIAFNQNNLVDEWRSL"
     gene            403..>897
                     /locus_tag="PLY93_13950"
     CDS             403..>897
                     /locus_tag="PLY93_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70628.1"
                     /translation="MIRRAFYYIFVEDLWAKLICLTIGIGIWAYVEFARVSQTTINVA
                     VEYIKKPVNLYLKSGQQRFVKITIRGRDEFLKFAPAALKAEVNLENAKSGEAKYPLIF
                     DARQLPERVDVLQKPDTLAVALERSAVKKVAIRAVTVGTVDTAWRLQKAIATPREIEI
                     EGPEA"
     assembly_gap    898..937
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <938..1423
                     /locus_tag="PLY93_13955"
     CDS             <938..1423
                     /locus_tag="PLY93_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70629.1"
                     /translation="IEGAARGFVRRVSLRLPDGISVDKSKGVAVRVDLIPKTYSEEKK
                     FEHIPIKVQNLDTALVATLSDTSVEVLVQGESNAIKSLGADEIYAYVNAEDTRYNPRT
                     GSILPYTNETNIPIRTRLLTSGKKIQIVAVTPENISIRYSIKPELTKKTQTPSTEKEN
                     K"
     gene            1432..>1801
                     /locus_tag="PLY93_13960"
     CDS             1432..>1801
                     /locus_tag="PLY93_13960"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRP70630.1"
                     /translation="MQPQIHLGINIDHVATLRNARGENDPSLVELALLAQNSGADSIT
                     IHLREDRRHVRDADIFELKKHLKIPINFEMALSEEILSIAQELKPMTVCLVPERREEV
                     TTEGGLDAVRYEERIQQFTAA"
BASE COUNT          509 a          397 c          438 g          417 t
ORIGIN      
        1 gctcattgta cttgtccaca aagtgggctt gaagcagtac gctgagtcgg gggtgcagat
       61 taacggcctc gtctcgaaag aatttttggt ttctatcttt tatggtaaaa accccttaca
      121 cgacggtgcg gtcatcctcg aaggccaaaa cgtcgttgct gctgcgtgtt atctccccat
      181 gagcacgtcg cgcgatgtga aacgcgcgca cggggctcgc caccgcgcgg cgttggggct
      241 ctccgaagat tcggatgcgc ttgtcattgt aatttcggaa acgagcggca aggtgacgct
      301 tgctttcttg ggagaaatga aagagaatat cgaccctgcg cggttacgta aaattttgat
      361 tgcgttcaat caaaataatc ttgtcgacga atggagatct ctgtgatacg ccgcgctttc
      421 tattatatat ttgtcgaaga tctttgggcg aaattaattt gcttaacgat tggtataggt
      481 atatgggcgt acgtcgagtt tgcgcgtgtc tcacagacaa ctatcaatgt cgccgtcgag
      541 tatatcaaga agccggtaaa tttgtatctg aaaagtgggc agcaacgctt cgtaaaaatt
      601 actattcgag ggcgggatga atttcttaaa tttgcgcccg ccgctttgaa agccgaagtg
      661 aatttagaaa atgcgaaaag cggcgaagcg aaatatccgt taatcttcga tgcgcgtcaa
      721 ctccccgaac gcgtcgatgt gttacaaaag cccgatactc ttgcggtggc attggagcgt
      781 tcagcggtaa aaaaagtggc tatccgtgca gttacggtag ggacggtcga tacggcatgg
      841 cgactccaga aagcaatagc gacaccgcgt gaaattgaaa ttgaagggcc cgaggccnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt gaaggtgcgg ctcgaggctt
      961 tgtgcggcgg gtgagtctgc gtttacccga tggaatatct gtcgataaaa gtaaaggcgt
     1021 tgcggtgcgc gtagatttaa tacccaaaac atacagcgaa gagaaaaaat ttgaacacat
     1081 acctattaaa gttcaaaatt tagatactgc actcgtagcc acattatcgg acactagtgt
     1141 tgaagtattg gtgcaagggg aatctaacgc cataaagtct ttaggcgcag atgagattta
     1201 cgcgtacgta aatgccgaag acacgcggta taacccacgc accggcagta ttttgccgta
     1261 taccaatgaa acaaatatcc ctatacgtac ccgcctttta accagcggta aaaaaatcca
     1321 aatcgtggca gtgactcccg aaaacattag tattcgctat tctataaaac ctgaactcac
     1381 taaaaaaaca caaaccccat ccaccgaaaa agaaaataaa tagagaaaaa tatgcaacca
     1441 caaattcatt taggcatcaa tatcgatcac gttgccacgt tacgtaacgc gcgtggagaa
     1501 aacgatccat ccttggtcga gttagcgctt ttagcgcaaa actccggtgc ggactcaatc
     1561 accatccact tgcgcgaaga ccgccggcac gtccgcgacg ccgacatttt tgaactcaaa
     1621 aaacacctca agatacccat caattttgag atggcgttaa gcgaggagat tttatcgata
     1681 gcgcaagagt taaaacccat gacggtgtgt ctggtgccag agcggcgcga agaggttacg
     1741 accgaaggcg gattggatgc ggtaagatac gaagagcgta tccaacaatt caccgcagcg
     1801 c
//
LOCUS       DAOMPD010000544         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_24977_length_1795_cov_3.245402, whole genome shotgun sequence.
ACCESSION   DAOMPD010000544 DAOMPD010000000
VERSION     DAOMPD010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24977_length_1795_cov_3.245402"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="PLY93_13965"
     CDS             <1..111
                     /locus_tag="PLY93_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70631.1"
                     /translation="CFWYYQSFPELVTGGLLADAVANLSSLNVIAGELDC"
     gene            complement(127..1668)
                     /locus_tag="PLY93_13970"
     CDS             complement(127..1668)
                     /locus_tag="PLY93_13970"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRP70632.1"
                     /translation="MKAVLNDFTRPIWEFLAWQTEFTADRVDIKDVTQDSREVKRGSA
                     FFAYDGLKFKGKDFVWEACTKSAAAIFVDFIYREEILNDERYKKEMPIFFAHDFLRSA
                     GRTISFLFGEPTRYMTTIAVTGTNGKTTVAHSLYQALNHLKKPAMYVGTLGMEVSRRK
                     ITTGMTTPDTVTLQSMLSQGQKQKALYACIETSSHGLEQGRLEGMNFQVGIFTNLTQD
                     HLDYHGTMESYYRAKRRLFEYMLFASQKNSDAVKGAIICIDDDYGKRLYAWLKSQNPS
                     FPVISLSLKEKSADALITSINADFQGYDATIVFAQKSYFIETKLLGRFNLMNLGCAFL
                     SLLCLGFSTQDALKAVEQVDPVPGRFEVLRGKEHRTVIIDYAHSPDALVKVLETAQEL
                     TPSRVIGLFGCGGDRDKEKRPLMAEVASLQADICILTNDNPRSEDPKRIIEDMRKGLK
                     TSRYLVITDREKAIRAGLQILGPRQVLVIAGKGHEDYQIIGNRTHHFSDREVASRLMS
                     TLKLK"
BASE COUNT          450 a          410 c          425 g          510 t
ORIGIN      
        1 tgcttttggt actaccaatc gttccccgag ctcgtcaccg gtgggctctt ggcggatgcg
       61 gtcgcgaact taagctcgct caatgtgatt gctggcgagt tggattgtta gcgtctcata
      121 agagccttat tttaatttaa gcgtactcat gagccgtgac gcgacttcac ggtctgaaaa
      181 gtggtgtgtg cggttgccga taatttgata atcctcgtgg cctttgcccg caatgactaa
      241 cacttgtcgt ggtcctaaaa tttgcaagcc tgcacgaatc gctttttcac gatcggtgat
      301 caccaggtag cgcgaagttt tcaagccctt gcgcatatct tcaatgatgc gtttagggtc
      361 ttcgctgcgt ggattgtcgt tggtcaaaat acatatatcc gcttgaaggc tagcgacctc
      421 cgccatgagt gggcgtttct ctttgtcgcg gtcgccgccg cagccgaaaa gaccaatcac
      481 ccgcgacgga gtcaattctt gtgcggtttc taaaactttg acgagcgcat cgggggagtg
      541 cgcgtagtcg ataatcaccg tgcggtgttc cttgccacgc aagacttcaa agcggccggg
      601 cactgggtcg acttgctcca ctgcttttag cgcgtcttgc gtggaaaatc ccaaacaaag
      661 cagagaaagg aatgcacaac ctaagttcat aagattgaac cgccccaaaa gttttgtttc
      721 aataaagtaa cttttttgag caaaaacaat cgttgcatca tagccttgga aatcggcgtt
      781 gatactggtg atgagcgcgt cggcagattt ctctttgagg ctcaaactaa tcaccgggaa
      841 agaagggttt tggcttttca gccaagcgta gagtctttta ccgtaatcat cgtcgatgca
      901 aatgatcgcg cctttgacag cgtcgctatt tttttgtgag gcaaagagca tgtattcaaa
      961 aagacggcgt tttgctcgat agtaagattc catcgtgccg tggtaatcga gatggtcttg
     1021 agttaaattg gtgaaaatac ccacttgaaa attcatgcct tcgagacggc cttgttctag
     1081 cccatgcgaa ctggtttcaa tacacgcgta aagagctttt tgtttttgtc cttggctgag
     1141 catactctgt agagtcaccg tgtcaggggt cgtcatgcct gttgttatct tgcgtcgtga
     1201 aacttccatc cctaatgtgc caacgtacat cgctggtttc ttcaaatgat tcaacgcttg
     1261 gtagagcgaa tgcgcaaccg ttgttttacc gttggtgcca gtgacagcga tcgtcgtcat
     1321 gtaacgcgtg ggttcaccaa aaagaaaaga aatagttcta cctgccgagc gcaaaaaatc
     1381 gtgtgcaaaa aaaatcggca tttctttttt atagcgttcg tcgtttagta tttcttcgcg
     1441 ataaataaaa tcaacaaaaa tagcggctgc actttttgtg caggcctccc acacaaaatc
     1501 tttgcctttg aacttgagac catcgtacgc aaaaaacgcc gaaccgcgtt ttacttcacg
     1561 gctgtcttgt gttacgtctt tgatgtcgac acgatcggcg gtgaactcag tttgccaagc
     1621 gaggaattcc catatcgggc gcgtaaaatc gtttaggact gctttcattt tatgctatct
     1681 cacaagttct taagatagag atgcgttgga aatactttaa cacgaagaac taataaacat
     1741 agcgttaggt aacaaaagtt aagaactcat ctaaaaattc acgccgctct tcata
//
LOCUS       DAOMPD010000545         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25002_length_1794_cov_2.138585, whole genome shotgun sequence.
ACCESSION   DAOMPD010000545 DAOMPD010000000
VERSION     DAOMPD010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25002_length_1794_cov_2.138585"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLY93_13975"
     CDS             complement(<1..283)
                     /locus_tag="PLY93_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70633.1"
                     /translation="MQKIYLIYLFCASGIIQFFFLAVHLFNLRSAQAKPLFIESLPIL
                     YLAATYALWLLVATFISWRTKKYRVPLLMHIPHLFSFLPLIRHIMLAADK"
     gene            complement(287..844)
                     /locus_tag="PLY93_13980"
     CDS             complement(287..844)
                     /locus_tag="PLY93_13980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRP70634.1"
                     /translation="MTVRELFTYRILTISNVLSISRILGIPAMWYILAHPHGIESIRL
                     YTGLTLLFMSATDFFDGYLARKLGQETPLGQYLDPIADKIVIIAGLFLLVAYRQFPLW
                     VAILVTLREIAGTIGGGFLLIKRNVLGKPNYWGKFGVGMISLSGLFYLFNWPYREYTI
                     PPLLFVFGGGIFAYAKTYGRTIFKG"
     gene            complement(849..>1005)
                     /locus_tag="PLY93_13985"
     CDS             complement(849..>1005)
                     /locus_tag="PLY93_13985"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRP70635.1"
                     /translation="PGWQEPTSHITKLKNLPKNARKYIDALAKYCGVPIQWISVGPGR
                     ENTIAIK"
     assembly_gap    1006..1015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1016..>1258)
                     /locus_tag="PLY93_13990"
     CDS             complement(<1016..>1258)
                     /locus_tag="PLY93_13990"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRP70636.1"
                     /translation="KAGNEFGSVTGRPRRCGWFDVEVVRHSARLNGLSALALTKIDVL
                     DNLKTIKVATGYELNGKRIDYFPSCAVNDIKIIYEE"
     assembly_gap    1259..1268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1269..1780)
                     /locus_tag="PLY93_13995"
     CDS             complement(<1269..1780)
                     /locus_tag="PLY93_13995"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRP70637.1"
                     /translation="MSEEASGSHKIGTTKRGIGACYSDKVNRQGIRIGMLYTDVFFNE
                     TLPRLVEQKNNYLTKVYNAPPTAFEPLKEKLLALREKIRDMVVNSPYYLNMELASGKR
                     ILLEGAQGTMLDLDFGTYPYVTSSNPTTGGALVGSGISFRYLKDVIGICKAYSTRVGE
                     GPYPTEILGE"
BASE COUNT          508 a          414 c          426 g          426 t
ORIGIN      
        1 gcttatccgc cgcgagcatg atgtgccgga tgagcggcaa aaaactaaag aggtgtggta
       61 tgtgcattaa aagcggcaca cggtattttt ttgtccgcca tgaaataaaa gtcgcgacta
      121 atagccacaa ggcatacgtg gcggctaaat aaagaatcgg tagcgactct ataaaaagcg
      181 gtttcgcttg cgcgctacgc aaattgaaaa gatgcactgc caaaaaaaag aattggatta
      241 tccccgatgc gcaaaaaaga tagatgagat aaattttctg catgcgctac cctttaaata
      301 tcgtacgccc ataggttttt gcatacgcaa aaatgccacc gccaaaaacg aaaagtaaag
      361 gtgggatagt gtattcacgg tagggccaat taaataagta aaaaaggccg gagagtgaaa
      421 tcattcccac gccaaattta ccccagtaat taggctttcc caaaacattg cgcttgatga
      481 gtaaaaaacc gccaccaatc gtccctgcaa tttctcgcag cgtcactaaa atcgccaccc
      541 aaagcgggaa ctgacggtag gcaacgagta aaaaaagacc ggcgatgatg acaattttat
      601 cggcaatggg atcgaggtat tgccccagcg gtgtttcttg tcccaattta cgcgctaaat
      661 aaccgtcaaa aaaatcggta gcgctcataa aaagtaacgt gagccctgtg tagagacgga
      721 tactctcaat gccgtgcgga tgcgccagga tataccacat cgctgggatt cccaaaatgc
      781 gcgaaataga aagaacgttg gatatggtga gaatgcggta ggtaaaaagt tccctgaccg
      841 tcatcgtatt attttatcgc aatagtattc tcacgccctg gcccgaccga aatccactgg
      901 ataggtacgc cacaatactt ggcaagcgcg tcgatatact tgcgtgcatt cttgggtaaa
      961 tttttaagtt tagtgatatg gcttgtaggc tcttgccatc ccgggnnnnn nnnnnattct
     1021 tcgtagataa ttttaatgtc attcaccgcg caagagggga aataatcaat ccgcttaccg
     1081 ttgagctcgt agccggtggc aactttaatc gttttgaggt tatcgagcac gtcaattttt
     1141 gtgagagcaa gcgcagataa gccattgagg cgcgcagaat ggcgcaccac ttccacatcg
     1201 aaccaaccgc aacgacgggg gcgtccggtg acgctaccaa attcgttacc ggctttacnn
     1261 nnnnnnnntc ttcgcctaaa atctcagtgg ggtaagggcc ttcgcccaca cgcgtcgaat
     1321 acgctttaca gatgccgatc acgtctttta aataacgaaa agagataccg ctgcccacaa
     1381 gcgcaccacc ggtggtgggg ttcgagctgg tgacgtaagg gtaggtgcca aaatccaagt
     1441 cgagcatcgt tccctgcgcg ccttcgagga ggatgcgctt ccccgacgcc aattccatgt
     1501 tgagataata gggagagttc accaccatat cgcggatttt ttcgcgcaac gccaacagtt
     1561 tttctttcaa aggttcaaat gcagtgggtg gagcattata aactttggtg agatagttgt
     1621 ttttttgttc aacgagacgg ggaagagtct cattaaaaaa aacatcggtg tagagcatac
     1681 caatgcggat gccttgccga ttcactttgt cggaataaca cgccccaatg ccacgcttgg
     1741 tggtgccgat tttatgcgac cccgatgcct cttcactcaa aacgtcgatt tgtt
//
LOCUS       DAOMPD010000546         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25157_length_1784_cov_3.259109, whole genome shotgun sequence.
ACCESSION   DAOMPD010000546 DAOMPD010000000
VERSION     DAOMPD010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25157_length_1784_cov_3.259109"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..416)
                     /locus_tag="PLY93_14000"
     CDS             complement(57..416)
                     /locus_tag="PLY93_14000"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP70638.1"
                     /translation="MATRDPKRRQDATVFAQGSAFKGTLEFAKPLRILGGYEGEIKGS
                     EYLEIGPEAKVNAHIDAAYVVIYGQVTGNVVATEKVELKEGAKLVGNIRSPKLEVDDG
                     VIFEGLCEMKSATEAVV"
     gene            complement(416..637)
                     /locus_tag="PLY93_14005"
     CDS             complement(416..637)
                     /locus_tag="PLY93_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70639.1"
                     /translation="MLSDQMQQVLQIYNEGLQLYKKREFKMAGEMFKKALGIKKDDGP
                     SQVYLERCEHFIAEPPPPDWDGVYVMKTK"
     gene            complement(1009..1372)
                     /gene="ssrA"
                     /locus_tag="PLY93_14010"
     tmRNA           complement(1009..1372)
                     /gene="ssrA"
                     /locus_tag="PLY93_14010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1564..1636)
                     /locus_tag="PLY93_14015"
     tRNA            complement(1564..1636)
                     /locus_tag="PLY93_14015"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Phe,seq:gaa)
BASE COUNT          457 a          414 c          376 g          537 t
ORIGIN      
        1 ttgttttttg agtattgaag ctggtttgta aaatccatca tcacaacaag tgggatttac
       61 accacagctt ctgtcgccga cttcatttca cacaaccctt caaaaataac cccgtcgtcg
      121 acttctaatt taggggaacg aatattcccg acaagctttg ctccttcttt gagttctact
      181 ttctcagtag cgaccacatt acctgtcact tggccgtaga tgacaacata cgcggcatcg
      241 atatgcgcgt taactttcgc ttcgggaccg atttctaaat actcgcttcc cttgatttca
      301 ccttcataac cgcctaaaat gcgcagtggc ttggcaaatt caagcgtgcc tttaaaagca
      361 gaaccttgtg caaaaacggt ggcgtcttgc cgacgctttg gatctcgagt cgccattatt
      421 ttgtcttcat tacgtaaacg ccatcccaat cgggcggggg tggttcagcg atgaagtgtt
      481 cgcaccgctc taaatacact tgcgatgggc catcgtcttt tttaatgccg agtgctttct
      541 taaacatttc acccgccatt ttgaactctc gctttttata gagttggagc ccttcgttgt
      601 aaatttgcaa cacttgctgc atttggtctg aaagcatggg catttgtatg gataaagcgg
      661 gcattgtaaa gattttttat tgttttgttc aaaataaatt tttactatgt agtcagacag
      721 aactcgctga tgcagaaagc gctatgaaaa tcttcgataa cctaaacata ctgttctcag
      781 agcggcaatg aatacccgcc ctccatcctc tgtttaacgc aagcttgcat gggggggtac
      841 tttttgtggc agcaattttt cgtttgtgtc ttttgtgcct ggtgggagta gttgaaggat
      901 ttttgtccct ctatgaaata gaggtaaaga aatagttcaa ttttctcctg cttatctata
      961 tttttttagg gtcgaaccct gcacgtaatt taaatacctc gtagaggaat ttggagacgg
     1021 cgggggtcga acccgcgtct tatcgttcta tcattcgctt tctacatgct tagtcgataa
     1081 ttgtttttca taaatggagt gcccatcgac aggcaatccc acttcttagt ggcttaactt
     1141 cggcaatgca tacgccacac aggcatcacc tattctgctg taaagttgag aaaatcgctt
     1201 ccgcagactc agacgatttt ccgtggctcg caaaagcgaa ccggctcagc cgcgattaag
     1261 cagcgagagc gtaagtttca gtttctgcag ttatacagat tgccttttta ttggaggcta
     1321 ccaagcatgc ttttcaaaca acagcgacga caatcgaagc caatgcgtcc cctaaagaca
     1381 ttccgcccac ggcgaaatgc ccgtggccgc tcaacgcgat ttttgcaacc cttttaggat
     1441 tagcgctgca gcggctttcg aattctatcg agcaatctgg aagagttata gcccaacgag
     1501 actactctat accaatagat acataatttg ccgctcagcg gcaaattatg tatcactttt
     1561 gtatggccag agagagaatc gaactcccga cacgaggatt ttcagtcctc tgctctaccg
     1621 actgagctat ctggcctaaa gaaaaaattc aaccagaagc aagagttaat tgcttcgcag
     1681 gcttattgac aatcttttga catgtcgtaa agcaaatttg ttttatttgg tatgcaaccc
     1741 acagagataa ctgacacttt tggggtcaag ttgagtgcgt ttta
//
LOCUS       DAOMPD010000547         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25460_length_1767_cov_3.469626, whole genome shotgun sequence.
ACCESSION   DAOMPD010000547 DAOMPD010000000
VERSION     DAOMPD010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25460_length_1767_cov_3.469626"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..617)
                     /gene="ribH"
                     /locus_tag="PLY93_14020"
     CDS             complement(144..617)
                     /gene="ribH"
                     /locus_tag="PLY93_14020"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR00114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRP70640.1"
                     /translation="MQVSDRFYSTPLKDKTYRIAVVTALFNHTITEPLAQGALDALFK
                     LGVDAAHIVQLTVPGAWELPLAAKKIFIDEKADAVIACGCVIQGDTGHYDIVANESAR
                     GLMQVALEFTRPVMNSVLTVKNYKQAKKRSQNDATNKGAEAARSLVELLNNFSQV"
     gene            complement(679..751)
                     /locus_tag="PLY93_14025"
     tRNA            complement(679..751)
                     /locus_tag="PLY93_14025"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Ala,seq:ggc)
     gene            complement(898..>1767)
                     /locus_tag="PLY93_14030"
     CDS             complement(898..>1767)
                     /locus_tag="PLY93_14030"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802980.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRP70641.1"
                     /translation="STREEREKALAKLLPFQRADFKGIFEAMEGLAVTVRLLDPPLHE
                     FLPNEEEQIEELAKTFGKSVHYVHKKIEDLKEFNPMLGHRGCRLGITYPEITAMQTRA
                     IIEAAELVKDGKKVFPEIMVPLVGHYKELANQAKVIREVAEAVQKEKGVKVDFLLGTM
                     IEVPRAAVTADEIASEAQFFSFGTNDLTQMTSGFSRDDSEHFLPYYIQKGIYPQNPFQ
                     VLDAGGVGKLIEIAIEKGRKTNPKIKLGVCGEHGGDPESIRFCHTVGLDYVSCSPYRV
                     PIARLAAAQAVLS"
BASE COUNT          418 a          425 c          430 g          494 t
ORIGIN      
        1 cggcaggcac agggtaatct acccaacgga atgcccccag ctcatcgacg gttgttgttt
       61 caaaaccgcg tacgtctgcc tgaaaaatcg cttgcatcgc caagatgcgt gctttgtgcc
      121 gatggcgtgt gctttttgcc atgttagact tgagaaaaat tgttaagcag ttcaacgagc
      181 gaacgcgcag cctcggcgcc tttgttcgtt gcatcgttct gagagcgctt tttagcctgt
      241 ttgtaattct tcactgtcaa tacggagttc atcacgggtc gcgtgaactc cagcgcaact
      301 tgcatgaggc cgcgcgcgct ttcatttgcg acaatgtcgt aatggccagt gtcgccttga
      361 atcacgcagc cgcaagcaat aaccgcatcg gctttttcat cgataaaaat ttttttagcg
      421 gcaaggggga gttcccacgc accaggtacg gtgagttgca caatatgcgc tgcatcgaca
      481 ccgagcttaa agagcgcatc gagtgctcct tgggctaagg gttctgtgat ggtgtggtta
      541 aaaagtgcgg tgacaaccgc aatacggtac gttttatctt taagcggtgt tgaataaaat
      601 ctatcggata cttgcatttt gtattctcag tattcttaat tctcaaaaaa attgaagtct
      661 cttaaagaaa tcaaagtttg gaggttaggg gagttgaacc cctgaccttc acaatgccat
      721 tgtgacgctc tcccaactga gctaaacccc cgtttgtaaa cggtggttac taagttttac
      781 atacacggtc aatcgcattc gaggttcatt acatctggaa aactttccgc ttggcgttcg
      841 gaagctatga ggagcatatc gattcactcc gcgcaaacgt gtgcggcgaa taaagaatta
      901 actcaacacg gcttgcgctg cggcaagccg tgcaatcggc acgcggtacg gcgagcacga
      961 cacataatct aagccgacgg tgtggcaaaa gcgtatcgat tctgggtcgc cgccgtgttc
     1021 gccgcaaaca cccaacttga ttttcggatt tgtcttgcgg cctttttcaa tcgcaatctc
     1081 aatgagttta cccacaccgc ccgcgtccag tacctggaat ggattttgtg gatagatacc
     1141 tttttggatg tagtaaggta aaaagtgttc gctgtcgtcg cgcgaaaagc cagaagtcat
     1201 ttgtgttaaa tcgtttgtac caaagctaaa gaactgcgcc tcgcttgcaa tttcatcggc
     1261 ggtaactgca gcacggggga cttcaatcat cgtgcccaaa agaaaatcga ctttgacgcc
     1321 tttttctttt tgtaccgcct cggcaacttc acggataact ttcgcttggt ttgcaagttc
     1381 tttgtagtgt cccacgagag gcaccataat ttcagggaaa acttttttgc cgtcttttac
     1441 taactctgcc gcttcgatga ttgcgcgtgt ctgcattgcg gtaatctcag gatatgtgat
     1501 gcctaagcga caaccacggt gtcccagcat gggattgaac tctttgaggt cttcgatttt
     1561 tttatgcaca taatgcaccg acttaccaaa cgtcttggcc aactcttcga tttgttcttc
     1621 ttcgttgggt aaaaattcgt ggagcggtgg gtctagtagg cgtacggtga ctgccaagcc
     1681 ttccatcgct tcaaaaatac ctttgaaatc ggcgcgttgg aacggtaaaa gtttcgcgag
     1741 cgctttttca cgctcttcgc gcgtgga
//
LOCUS       DAOMPD010000548         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25825_length_1744_cov_2.002368, whole genome shotgun sequence.
ACCESSION   DAOMPD010000548 DAOMPD010000000
VERSION     DAOMPD010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25825_length_1744_cov_2.002368"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..>746)
                     /locus_tag="PLY93_14035"
     CDS             complement(276..>746)
                     /locus_tag="PLY93_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017855632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRP70642.1"
                     /translation="VTTTGNKDIITIEHIKKMKDQAIVCNIGHFDNEIQVTALENLTG
                     VKKLNIKPQVDKYTLPNGNSIYLLAEGRCATGHPSFVMSNSFTNQTLAQLDLWQNRDT
                     YKIGVYRLPKHLDEEVARLHLERIGVKLTTLSKEQADYIGVKVEGPYKPEHYRY"
     assembly_gap    747..782
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<783..>1522)
                     /locus_tag="PLY93_14040"
     CDS             complement(<783..>1522)
                     /locus_tag="PLY93_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRP70643.1"
                     /translation="IQTAVLIETLKELGADVRWASCNIFSTQDHAAAAIAKTGTPVFA
                     WKGETLEEYWWCTEEALTWPDGSGPDLIVDDGGDATLLIHLGTQAEKDSKILEQKTDN
                     KEFQIILNLLKKSLATTPDKWTKFGETMRGVSEETTTGVHRLYQMLEKGELLFPAINV
                     NDSVTKSKFDNLYGCRESLADGIKRATDVMLAGKVAVIAGYGDVGKGSAQSLRNFGCR
                     VLVTEIDPICALQAAMEGYQVTTMEDALT"
     assembly_gap    1523..1535
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1536..1722)
                     /locus_tag="PLY93_14045"
                     /pseudo
     CDS             complement(1536..1722)
                     /locus_tag="PLY93_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476655.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
BASE COUNT          410 a          371 c          389 g          525 t
ORIGIN      
        1 gtaaactaat cgatgtcttt aacatatttg cctctatata ttaaatacca gaaaacggtc
       61 aaattttctc attttgagaa aaaaaagtac aatttttgga aaaaattgta cttttttttt
      121 ggcagaaatc attcgtgtgg atagttatcc cctaatgtga cataatccaa atttaaggta
      181 gcccaagcat atccagattt tgaaaaactt ataatttttg atatttttac gcactgtgtg
      241 cataaaaata tcaaaaaatt cagtattttg aggggttaat accgatagtg ctcgggcttg
      301 tatggccctt cgaccttgac tccgatgtag tcagcttgtt ctttcgataa tgtcgttaat
      361 ttgacaccaa tacgctctaa atgcaaacgc gctacctctt cgtctaagtg tttcggcaag
      421 cgatacaccc ctattttgta ggtgtcgcgg ttttgccata gatccaattg cgcgagagtt
      481 tgattggtaa acgagttgct catcacaaaa gagggatgac cggtggcgca acggccttcg
      541 gccaaaagat aaatcgaatt accgttaggg agcgtatatt tatccacttg cggcttgatg
      601 ttgagctttt tgacgcctgt taaattttca agcgcggtta cctgaatttc gttgtcgaag
      661 tggccgatgt tacacacaat cgcttggtct ttcatttttt tgatgtgttc aatggtgatg
      721 atgtccttat taccggtggt ggtcacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nngtgagagc gtcttccatc gtggtgactt gatagccttc cattgccgct tggagtgcac
      841 agataggatc gatttcggtg acgagtacgc ggcaaccaaa attccggagc gattgcgccg
      901 aacccttacc gacatcgcca taccccgcaa tcactgcaac ttttcctgca agcatcacgt
      961 cggtggcgcg tttgatacca tcggcgagcg attcacggca accgtaaagg ttatcaaatt
     1021 ttgatttcgt gaccgagtcg ttcacgttga ttgcgggaaa aagaagctcg cctttttcga
     1081 gcatttgata gaggcggtga actcccgtag tggtctcttc ggaaacacca cgcattgtct
     1141 caccaaattt tgtccactta tcgggagttg tggcgagtga ttttttgagt aagttcaaaa
     1201 taatttggaa ttctttattg tcggtttttt gttccaggat tttggagtct ttctctgcct
     1261 gtgtgcctaa atggatcaac agcgttgcgt cgcccccgtc gtcgacgatg aggtctgggc
     1321 ctgaaccgtc cggccatgtg agcgcttcct cggtacacca ccagtattct tcgagggttt
     1381 cgcctttcca cgcaaaaacg ggtgtgccgg tttttgcaat ggcggcagcg gcgtggtctt
     1441 gtgtagaaaa aatattgcag ctcgcccagc gcacgtctgc gcccaattct tttagggttt
     1501 caatcaagac ggcggtttgt atnnnnnnnn nnnnnagccc ataatctttg cgcctttaag
     1561 aggtttcgat gcgccatatt tttttctgag cgacatgagc cctggcattt catgctcggc
     1621 aatatcgatc tctttgcggc caaaatcggc taagttgatg tcggcaactt tatacgggag
     1681 ttgaccgata gcaaccccag aggtatgttc tacatttttc atatcgatac atttacatac
     1741 atcg
//
LOCUS       DAOMPD010000549         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25861_length_1742_cov_3.330765, whole genome shotgun sequence.
ACCESSION   DAOMPD010000549 DAOMPD010000000
VERSION     DAOMPD010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25861_length_1742_cov_3.330765"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLY93_14050"
     CDS             complement(<1..336)
                     /locus_tag="PLY93_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70644.1"
                     /translation="MTKIKVSAFSVTLDGYSAGPKQSLENPLGERGGEMMNWVFPTRF
                     FQKMHGAVDAKTDAPSGVDSEASLPSSDNNGTTGIDNDFAMRSFENVGAWILGRNMFG
                     PVRGPWPDEE"
     gene            complement(510..1172)
                     /locus_tag="PLY93_14055"
     CDS             complement(510..1172)
                     /locus_tag="PLY93_14055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRP70645.1"
                     /translation="MAVWENTASLFDLPHASFDAVIGVDEVGRGALFGPVTVGAVYLT
                     RTLCTQLSSQDWLHKVNDSKLLNAAQRTKVAPLLANFFSHAIQHTAVSYLDRYNISRA
                     VDRGIYKSVQKILEKVEIPLERVYVLFDGKIIPHYPQLLLQKPMPFLKCEVKADRHFF
                     PVAAASILAKVARDTLIARAAERFPQYGLEKHAGYGTQRHRQAIIEYGLTKFHRRSFR
                     VS"
     gene            complement(1187..1636)
                     /gene="rplS"
                     /locus_tag="PLY93_14060"
     CDS             complement(1187..1636)
                     /gene="rplS"
                     /locus_tag="PLY93_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRP70646.1"
                     /translation="MLNQILEKLHYDVVSKPVDFSVGDTVKVHYKIKEGQKERIQIYE
                     GLVIAIQNQGAGKTFTVRRVSFDVGVERIFPLYAPTVTRVERIRSSKVRRAKLYYLRE
                     KSGKESRLKELPRKPIKDPLYEKAQAYKASKPVATAATPAAEPAAAE"
BASE COUNT          431 a          400 c          428 g          483 t
ORIGIN      
        1 ttcctcgtcg ggccaaggcc cgcgcaccgg gccgaacatg ttgcgtccta aaatccatgc
       61 gcccacattt tcaaacgacc gcatggcgaa atcgttgtct attccggtcg taccgttgtt
      121 gtcgctcgaa ggcaaggatg cctcagagtc gacgcccgat ggagcgtcgg tcttcgcgtc
      181 gaccgcaccg tgcatctttt gaaaaaatcg cgtcgggaaa acccagttca tcatctcgcc
      241 cccgcgctcc cccaagggat tttcgaggct ctgcttgggc ccggcggagt agccatcgag
      301 agtcacagaa aatgccgata ctttgatttt tgtcattatt tcacctatat gttaattaac
      361 tgataggtaa aataataaat ttcattaagt gcgcctaaaa aattgtataa ttaccgttaa
      421 gaaatgggct ccgtaaggcg tggcatatta accgtcgacc ttgagatcga atggttcgtc
      481 aagttctacc actgtcaccg catccctttt taagaaacac gaaacgaacg ccgatgaaac
      541 tttgttaatc cgtactcgat gattgcttgc cgatgccgtt gggtaccata tcccgcatgc
      601 ttttccaggc cgtactgagg aaaccgctcc gccgcgcggg cgatcagcgt gtcacgggca
      661 accttggcga gtatcgacgc tgccgccacg ggaaaaaaat gccggtcggc ttttacctcg
      721 cattttaaga atggcatggg cttttgtaat aacaattgtg ggtagtgtgg tattattttt
      781 ccgtcaaata agacgtaaac acgctccagt gggatttcaa ctttttctaa aattttctgc
      841 actgacttgt agattcctct atcgacggca cggctaatgt tgtagcgatc gagatacgac
      901 acagccgtgt gttggattgc atgcgaaaaa aagttagcaa ggagtggagc aacttttgta
      961 cgttgcgcgg catttaaaag ttttgaatcg ttgactttgt ggagccagtc ttggcttgaa
     1021 agttgagtac agagggtgcg ggtgagataa accgcaccaa cggtgacagg gccgaaaaga
     1081 gcgcctctac ccacttcgtc gacaccgatg acagcatcga acgacgcatg gggtaaatca
     1141 aatagcgatg cggtgttctc ccacaccgcc attctttgat ttaattttat tctgcggcgg
     1201 caggttcagc cgccggggta gctgcagttg ccacaggttt agacgctttg tatgcttgtg
     1261 ctttttcgta aagaggatct tttattggtt tacgtggcag ttcttttaag cggctttctt
     1321 tacccgattt ttcgcgtaaa taatagagct tcgcacggcg tactttactt gaacgaatgc
     1381 gctcgacacg tgtaacagtg ggtgcataaa gaggaaaaat acgttcaacg ccgacatcga
     1441 agctaacacg acggacggta aaggtcttac cggcgccttg attttgaatc gcaatgacaa
     1501 gaccttcgta aatttggata cgttcttttt ggccttcttt gattttgtaa tgtactttaa
     1561 cagtgtcgcc tacggaaaag tctacgggtt tagagactac gtcgtagtgc aatttttcta
     1621 aaatttgatt aagcattctc cttgatagct atttgagaca ggccgtcaag gagtctgtaa
     1681 ggtggtacta tgcaacccac agagataact gacacttttg gggtcaattt gagtgcgttt
     1741 ta
//
LOCUS       DAOMPD010000550         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25883_length_1741_cov_3.019573, whole genome shotgun sequence.
ACCESSION   DAOMPD010000550 DAOMPD010000000
VERSION     DAOMPD010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25883_length_1741_cov_3.019573"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PLY93_14065"
     CDS             <1..1587
                     /locus_tag="PLY93_14065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HRP70647.1"
                     /translation="RTAQTRRSGHPLRKLQRIMAFENDTVFLIHFNDVYRKKIQEDTL
                     SPLLALFAGKLNAFNAPVYLCTPDGGAADKIATHLPGAEKLNLQSEIRTVRHLFSTTK
                     ATHIGIFNGAYPLFDKSLTQKIFQLHSEYRADISYGENLPAGMAPYFVSRDLLESLDI
                     IEAKDEEWQENGIRAFVEKNINQFHAEVHYEEPDLRLLRLDFSLSTKRSVQKAKAFLE
                     KILDKENPYHELQHLIDGAPQLLATFPSYIELEFNSQSEHLSFFSPLKYIEQEKHLLS
                     RENFARVKNYIATGLGDTSVCASGLGEPLEHPDALYFIEELLGDNNIPYVFIETNGVR
                     LDALFSLAAHPHADKLRAIVFLNSLEKYAEYSGAPANTLEKVKNNVRAFVIKLREAKR
                     NPQEILYIQALKVEENETEIDALYALTEELGATFLFQKYNRYAGLMPERRVSDMTPLE
                     RYSCWHLRRDIFIRANGDVAYCKQTIDQKKNTARGNLANDDLIDLWNAQRDDFEKNYK
                     GDYPPHLPCATCDEYFTFNF"
BASE COUNT          501 a          407 c          386 g          447 t
ORIGIN      
        1 agaacagcgc aaacgcgtcg cagtggacat ccgcttagaa aactacaaag aataatggcg
       61 tttgagaacg acacagtttt tctgattcat ttcaacgatg tgtatagaaa aaaaattcaa
      121 gaggatacac tctcccccct cctcgctctt tttgccggta agctaaacgc gttcaacgcg
      181 ccggtgtatc tttgtacgcc cgacggcggc gccgcagata aaattgccac gcatttacct
      241 ggtgccgaaa aattaaattt gcaaagtgaa attagaacgg tgcggcattt gttttcaacg
      301 acgaaggcga cgcacatcgg catcttcaac ggcgcttatc ctctttttga taaaagttta
      361 actcaaaaaa tttttcaact gcatagcgag taccgcgcgg atattagtta cggtgaaaat
      421 ttaccggcgg ggatggcgcc atattttgtg agccgcgatt tattggagtc gctcgacatt
      481 atcgaagcta aagacgaaga gtggcaagag aatggcatcc gtgcgtttgt tgaaaagaac
      541 atcaaccagt tccatgcaga ggtgcattac gaagagccgg atttacgtct gttgcgctta
      601 gatttttcgt tgtcgacaaa acgctccgta caaaaagcaa aagcatttct tgaaaaaatt
      661 ctagacaaag aaaacccata tcacgaattg caacacctta tcgacggagc gccgcaactc
      721 ctcgccactt tcccgtcgta tatcgagtta gagttcaact cgcaaagcga gcacctaagt
      781 tttttttcac cgcttaaata catcgaacaa gaaaaacatt tgctttcgcg tgaaaacttt
      841 gcgcgtgtaa aaaattatat cgccacgggg ttgggcgaca cctcggtgtg tgcatcgggg
      901 ttgggcgagc ctttagagca ccccgacgcg ctttatttta tagaagagtt gctcggcgac
      961 aacaacatcc cttacgtatt tatcgaaacg aatggtgtcc gcttagacgc tttgttctcc
     1021 ctcgccgcgc accctcatgc ggataagcta cgtgcgattg tttttttaaa ctcgctggag
     1081 aaatacgcag agtattctgg cgcgcctgcg aacactttag aaaaagtgaa aaacaatgtg
     1141 cgcgcctttg taataaaatt gagagaagcc aaaagaaatc cgcaagaaat tctttatatc
     1201 caagcgctca aagtggaaga gaacgaaacc gaaatcgacg cgctctatgc actcaccgaa
     1261 gagttgggtg cgacttttct ttttcaaaaa tacaaccgtt acgctggcct catgcccgag
     1321 cggcgcgtct ctgacatgac gcctttagag cgttattcgt gctggcattt gcgccgtgat
     1381 attttcatcc gcgcaaacgg agatgtagcg tattgcaagc aaaccattga tcagaaaaag
     1441 aacacggcgc gaggcaatct tgctaacgac gacctcattg atttatggaa tgcacaacgc
     1501 gacgattttg aaaaaaatta caaaggtgat tacccacctc acttgccgtg cgcaacgtgc
     1561 gacgagtatt ttacctttaa tttttgattt aacgactctt acttgaccgg cgtgctctca
     1621 aaaaaaataa tgagtcatgc gcaaaatctg cttttttctc tctctgctct ttggatgtgc
     1681 cgttattatc accgcctgca aagatacgaa taaagtcacc gaagccgaga ctgctgtagt
     1741 a
//
LOCUS       DAOMPD010000551         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_25889_length_1741_cov_2.626928, whole genome shotgun sequence.
ACCESSION   DAOMPD010000551 DAOMPD010000000
VERSION     DAOMPD010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25889_length_1741_cov_2.626928"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLY93_14070"
     CDS             <1..674
                     /locus_tag="PLY93_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70648.1"
                     /translation="KGAYTLNVTFTAVTPATPGSLLVGGGTSGGADGIVTITNDDTPP
                     LVKATGKKLHPTRQLTLDIARTYVGRISDLGGTGGNKLGWELLKDIYDTSYHTNVFSW
                     GQTAIDGIKLPGKKINSWCGIFAVAMVRKAGVDAANWQTGIGPKGIASSCKANDGNFT
                     PGDILVMKGSLVHHNLFVSQKTDGSMVTIDGNQVYQTVKLRTNLNASDVQCYYTPLTE
                     KYVDE"
     gene            complement(1042..>1741)
                     /locus_tag="PLY93_14075"
     CDS             complement(1042..>1741)
                     /locus_tag="PLY93_14075"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRP70649.1"
                     /translation="SRIFQFILAFGAQTTGQKGVLWWAGHHRNHHKYSDTEKDVHSLK
                     IRGFYTSHIGWIFDKNNKRVPIEEIPDFAKYPEIRFISKHDWICPWTLGFFSFLFAGW
                     PGLWIGFFLSTLLTYHSTFTINSLTHKWGKKRYETGEESKNHWLLAITTMGEGWHNNH
                     HYYQGSAKMGFFWWEIDMTYYLLRFLSLFGLVWNLKGVPKAVRAKTIQHQAKLKAEAE
                     RTPTPLKQLVPEET"
BASE COUNT          447 a          381 c          431 g          482 t
ORIGIN      
        1 gaaaaggcgc gtacacgctt aacgttacat ttaccgcagt gacacctgcg acacccggca
       61 gcttgcttgt gggtggcggc acatcgggcg gcgccgatgg tattgttacc atcacgaacg
      121 acgacacacc gccgttggtg aaagcgacgg ggaaaaaact ccatccaacc cggcagttga
      181 cgctggatat tgcacgcact tacgtgggac gcatcagcga cttgggtggt acgggtggta
      241 ataagctggg ttgggagctc ttgaaagata tttatgatac ctcgtaccat acgaatgttt
      301 ttagctgggg gcaaacagcc atcgatggta tcaagctccc tggtaaaaaa ataaattcgt
      361 ggtgtggtat ttttgccgtc gcgatggtac gcaaagcggg cgtcgatgcg gccaactggc
      421 aaaccggcat cggcccgaaa ggcatcgctt cttcttgcaa agccaacgac ggcaatttta
      481 ctccgggtga tattttggtg atgaaggggt ctttagtaca ccacaatctc tttgtctccc
      541 aaaagaccga cggatcgatg gtgaccatcg acgggaatca ggtttaccaa acggtcaagt
      601 tacgcactaa tctgaatgcg tcggacgtgc agtgctatta tacaccgctt actgaaaagt
      661 atgtggacga gtgatttttg ttttacagga gagacaacag ttatttataa actagaccta
      721 ttgcatcggt gctctaaaca aaatctttgt tttttcttca ttatttatta tttacgataa
      781 agagaatcca ccgttcgtaa cacgaagaca ataggcgttt tctctccctt caagtttttt
      841 gatatccggc ttttccaagg attttacttt tttgctatcg tagatagtga gcctgtgcgc
      901 caactcatgc agtgtgtaca aaaaaattgc ctcagcaagt tggcgcataa gctatagtat
      961 gaattgcgcg catatagatt tattaaaact gctctttcgc agttaaagta taaacaaata
     1021 tgcgcggatg agtaatttgt attaagtctc ctccggcacc aactgcttca acggcgtcgg
     1081 tgtgcgctcc gcttctgctt ttaattttgc ttggtgctga attgtttttg cacgcactgc
     1141 tttaggaact cctttaagat tccagacgag accaaagaga ctcaaaaagc gcaagaggta
     1201 gtaggtcatg tcaatttccc accagaaaaa tcccattttg gccgagcctt ggtagtagtg
     1261 gtggttattg tgccaacctt cgcccatggt ggtgatcgcc aagagccaat ggttttttga
     1321 ttcttcgccg gtttcgtaac gctttttacc ccacttgtgc gtgagtgagt tgatggtgaa
     1381 cgtcgagtgg taggtgagta gggtgctcaa gaaaaaacca atccacaatc ccggccagcc
     1441 cgcaaacaag aacgagaaaa atcctaatgt ccacggacaa atccaatcgt gtttagagat
     1501 gaatcgaatt tctggatatt ttgcaaaatc ggggatttct tcaatgggta cgcgcttatt
     1561 gtttttgtca aaaatccaac cgatgtgcga tgtataaaaa ccgcgtattt tgagcgagtg
     1621 aacgtctttt tctgtgtcgc tgtatttgtg gtggttgcgg tggtgaccag cccaccagag
     1681 cacgcctttt tggccggtcg tttgcgcgcc gaaagcgagg atgaactgga atatgcgact
     1741 g
//
LOCUS       DAOMPD010000552         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26176_length_1726_cov_2.785159, whole genome shotgun sequence.
ACCESSION   DAOMPD010000552 DAOMPD010000000
VERSION     DAOMPD010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26176_length_1726_cov_2.785159"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLY93_14080"
     CDS             complement(<1..197)
                     /locus_tag="PLY93_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRP70650.1"
                     /translation="MIYSLGTQIPQIDPMAWIASSATLLGDVHIGAESSLWYGSVVRG
                     DVHSIRIGSQTNIQDLSLIHVT"
     gene            complement(237..449)
                     /locus_tag="PLY93_14085"
     CDS             complement(237..449)
                     /locus_tag="PLY93_14085"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRP70651.1"
                     /translation="MIICSCARVNDKQIRHALAHGSRTIADLQNKLGVSMHCGKCREA
                     VQEEITKFYGASEPPHYFPSQLAPTN"
     gene            complement(636..1040)
                     /locus_tag="PLY93_14090"
     CDS             complement(636..1040)
                     /locus_tag="PLY93_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70652.1"
                     /translation="MSEGKRPTVATVFGVLNIVFGAFGVFGIFGALAWFTLGAKILGV
                     LSIGSTLGSLLLLFSGILLVANKDNALKITNLAIIVSLALTAASIIYGVAAGYQTMGA
                     VITTIILSLVYPALIFFLLLKNAEVQSFYGSR"
     gene            1225..>1726
                     /locus_tag="PLY93_14095"
     CDS             1225..>1726
                     /locus_tag="PLY93_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRP70653.1"
                     /translation="MPDFKKLRSEFSLDKNLLWLNNCGISVPPQICATTTAAYLDAYA
                     REGVRQTFRPHGVTKRNIQNYLVKLFGGTAHEYALLNNTAEGMTFIAQSLKIKAGDKI
                     LLVAREYPSNVYPFMQLKHQGVELEFIETANDNATFLKNIEKALTPRVKAMSLSAVDW
                     LTGLRFD"
BASE COUNT          540 a          394 c          350 g          442 t
ORIGIN      
        1 gtgacgtgga tgagactcaa atcttggatg ttcgtttgac tgccgatgcg tatggagtgg
       61 acatctccgc gcaccacgct gccataccag agtgagcttt cggcaccgat gtgcacgtca
      121 cctaaaagcg ttgccgagga ggcaatccag gccattggat cgatttgagg aatttgggta
      181 ccaagcgaat agatcatttg tggtaaaatt tcatttccac aagcgaacgc acacccttaa
      241 ttggtcggcg ctaattgtga aggaaagtaa tggggtggct cgctggcgcc gtagaatttc
      301 gtgatttctt cttgaacagc ttcacggcat ttaccgcaat gcatggaaac accgagtttg
      361 ttttgtaaat cagcaatggt gcgtgaacca tgggcaagag catggcggat ttgtttatca
      421 ttcacgcggg cgcaagaaca gataatcata cgctactgag aataagtctc atttgccgta
      481 aagaataatt tactttttcg attctttaat taaaattgaa ttatctaaaa aatcaaattt
      541 ttaaactgtc tcataggtgc ttacgtacct atgagacagt cgcaattaaa aaggctgaat
      601 ttcgggatta aacaactccc attccagtga tacgcttagc gtgaaccgta gaacgattgt
      661 acctcagcat tcttaagcaa caagaaaaaa atgagcgctg gataaacaag actcagaatg
      721 attgttgtaa ttactgcacc cattgtctga taaccggcgg caacaccgta aataattgaa
      781 gccgcagtta acgcgagcga cacaataatg gccaaattcg taatttttaa cgcattatcc
      841 ttattggcaa cgagcaatat acccgagaaa agtaaaagta gcgaacccaa tgtgctacca
      901 atgctcaata ctcctaaaat ctttgctccg agtgtaaacc aagcgagtgc accgaagatt
      961 ccaaaaaccc caaatgctcc aaacacaatg tttaatacac caaaaacagt cgcaaccgtt
     1021 ggtctttttc cttctgacat aaattcctcc aaaaaatgtt ttaataccaa aacgaaagag
     1081 atgttcaaaa aatcctaaat tttgatacgg acaaacacaa aacgatgggc aagtaatttg
     1141 tcaatgagtt attcatataa aaatataatc caatgactaa gatgtatagc cacagtgttt
     1201 aatttttctg tattaactac aattatgccc gactttaaaa aattacgcag cgaattttct
     1261 ctggataaaa atctactctg gctcaataat tgcggcatca gcgtcccgcc ccaaatttgc
     1321 gccaccacaa ccgccgcata cctcgacgct tacgcgcgcg aaggtgtccg tcaaacattc
     1381 cgcccacacg gcgtcactaa gcgcaatatc caaaattatt tagttaagct ctttggcggc
     1441 actgcgcacg aatacgcact cctcaacaac accgccgagg gcatgacgtt tatcgcacaa
     1501 agcttgaaaa taaaagcggg cgataaaatt cttctcgtgg cgcgtgaata ccccagcaat
     1561 gtttatcctt ttatgcagct taaacaccaa ggggtggagc tggaatttat cgaaacggca
     1621 aacgataacg ccacgttcct aaaaaatatt gaaaaagcgc tgactccccg cgtgaaggcg
     1681 atgtcgcttt cggcagtcga ttggctcact ggcctccgct ttgatt
//
LOCUS       DAOMPD010000553         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26220_length_1724_cov_2.092271, whole genome shotgun sequence.
ACCESSION   DAOMPD010000553 DAOMPD010000000
VERSION     DAOMPD010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26220_length_1724_cov_2.092271"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PLY93_14100"
     CDS             <1..1233
                     /locus_tag="PLY93_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70654.1"
                     /translation="PQMFQNYFDATAAAANEPNVNLKKNIMRTRLNFNRNKHPSKLRY
                     DASSYDGRVEYNFVVADKTTCWVSTGGANAATFSTGLSIVFVFHSFDICNDFYNEAQQ
                     GAYGGLFGDEGEPSFGKFRFNKTITDPNTRFRLGDLIFNLYFAPQEYPQTAVVTELLR
                     AEKSIQFAARAMTQDIITDVSDHTANRSHLLNAFQYKSTIPKLYGNNFSIRGIFGTEI
                     DAAPTPLATPWTAVTQPYNALVSESCPTLNTTAAAALTLPFYDGSSISSPGNTYCHAG
                     GTSNLAGPSCSGSSTLVNLTSIHCDMANLMTSVGDASVVSVKKLSSPIPYNVFLTDYG
                     ARRPRLIVMSSDLRKRYYYDKGGSQDVEPKRTRDDFFLITDAVVMVIEPAGSQTNTKI
                     FDDFKALIDKLMQAGGAL"
     gene            1272..>1724
                     /locus_tag="PLY93_14105"
     CDS             1272..>1724
                     /locus_tag="PLY93_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRP70655.1"
                     /translation="MACSRVTVSEAEPNNRLLEIYTNYPGKAPNSHRPPEFDIRLANL
                     IDNAKKEVYWAMYGFYRASIKDSVLRAVRRGLDVQLAGDAGTYSYGEIGYVQFEDLLR
                     RYPNAKLIAGNSQSIQHNKFCIIDRRYVMTGTGNITDSEIDRNYNVSTI"
BASE COUNT          436 a          443 c          415 g          430 t
ORIGIN      
        1 ccacaaatgt ttcaaaatta tttcgacgcc accgccgctg ccgccaatga gccaaatgtg
       61 aatctcaaaa aaaacattat gcgcacgcgg cttaatttta ataggaataa gcaccccagc
      121 aaactacgtt acgatgcgag ctcgtacgat ggccgtgtcg aatataattt tgtcgtggcg
      181 gataaaacca cctgttgggt atcaaccggt ggcgcgaacg ccgcaacatt ttcgaccggt
      241 ctttcgattg tttttgtctt ccactcgttc gatatctgca acgactttta caacgaagcg
      301 cagcaaggcg cgtacggcgg gcttttcggc gacgaaggcg aaccgtcgtt tggtaagttt
      361 cgttttaata agacaatcac cgaccccaac acgcgttttc ggctggggga tttgattttt
      421 aatttgtact ttgctccgca agaatacccg caaaccgccg ttgtcaccga gcttttgcgt
      481 gcggaaaaat cgatccaatt tgcggcacgc gcaatgacac aagacattat caccgacgtg
      541 agcgatcaca ccgctaaccg tagtcatctt ttgaacgcat ttcaatataa atccacaata
      601 ccaaaactct atgggaataa ttttagcatc cgtggtattt ttggcacaga aatcgacgcc
      661 gcaccgacac ccttggcaac accgtggacg gcggtgacgc aaccgtataa cgcattggtg
      721 tcggaaagtt gccccaccct caacaccaca gcggcggcgg cgctcacatt acctttttat
      781 gacggcagct ctatttcaag cccgggaaat acctattgcc acgcaggcgg gacttccaat
      841 ttggcaggcc ccagctgttc gggctcgtcg acgcttgtaa atctaacgtc gatccattgc
      901 gatatggcga atcttatgac ctccgttggc gatgcgtcgg tggtgtcggt aaagaaatta
      961 tcgtcaccga ttccttacaa tgtctttttg accgattacg gtgcacgccg tcctcgcctc
     1021 attgtgatgt cttccgattt gcgtaaaaga tattattacg acaaaggcgg ctcgcaagac
     1081 gtggagccca aacgtacgcg cgacgatttc tttttaatta ccgacgcggt ggtgatggta
     1141 atagagccag cggggtcgca aacgaacaca aaaatctttg atgactttaa ggcgcttatc
     1201 gataaactca tgcaagcggg tggcgcttta tgaaacgatt gattttgtgt gtatgtatat
     1261 tcattcccct tgtggcgtgt tcgcgcgtga ccgtgagcga ggcagagccc aacaaccgcc
     1321 ttttagaaat ttacaccaac taccctggga aagcgcccaa cagccaccgc cctcccgagt
     1381 ttgacatccg tttagcgaac cttatcgaca atgccaaaaa ggaagtctat tgggcgatgt
     1441 acggttttta ccgtgcatcg attaaagatt cggtgctgcg tgcggtgcgc cgtggcctcg
     1501 acgtgcagtt ggcgggcgac gcgggaacgt actcgtatgg cgaaatcggt tatgtgcagt
     1561 ttgaagattt actgcgccgc tatcccaacg ccaaactcat tgcaggaaat tcgcagtcga
     1621 tccaacacaa taagttttgt atcatcgacc gccgctatgt gatgacggga acaggaaata
     1681 tcaccgattc ggaaattgat cgcaactaca acgtgtcgac gatt
//
LOCUS       DAOMPD010000554         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26346_length_1716_cov_3.287176, whole genome shotgun sequence.
ACCESSION   DAOMPD010000554 DAOMPD010000000
VERSION     DAOMPD010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26346_length_1716_cov_3.287176"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1716
                     /locus_tag="PLY93_14110"
     CDS             111..>1716
                     /locus_tag="PLY93_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRP70656.1"
                     /translation="MAFNTINEIFYYAGDVYGAREMFFAKNEKKDFIGMTFGEVLKRA
                     ENTAIALMDMGVERGEKIGYLADNRTEWIIGDIASLLTGGVTVPRGTDVTIDEIKYIM
                     AHAECKFCFVENEATLKKLQSVIKDTKVEKIIVLDPKFKGTNENIFTLQQLVDAGAKV
                     RKEKLPALKKRVESIQPDDLFTIIYTSGTTGLPKGVMLSHGNMVYNINEVPKAIGLRP
                     DDSMLSLLPVWHIFERAVDYGAISKGIPIYYTNVRDVREDFTKVKPTFMPSAPRLWES
                     LYQGIEQRIQKSEPARKMLFEAAYAINKAYKKATDYLAGNELMMKPETDLERLTRTAQ
                     ALFVVANLHFPARLADKVVFGKIREAMGGRFRGSISGGGALPAHVDEFFNVIGVPVYE
                     GYGMTECAPIISVRIEGRIIQGSVGFVPNGTEVAILNDKGERVKTGEKGVIHVRGPQV
                     MKGYYKNPEATEKTISKDGWLNTGDIGFFSFNNTLSIRGRAKETIVLLGGENVEPVPI
                     ENKLLEDAQINQVIVVGQDKKSLTALI"
BASE COUNT          489 a          384 c          430 g          413 t
ORIGIN      
        1 ccacctacct taaacacttt ataaaaagtg taacatatgt cttgagggtt tagactccta
       61 agtcgtacaa attgttgacc gtgctgccca agatgacgat taatccaccc atggcattta
      121 atacaatcaa tgaaattttt tattacgcgg gggatgtgta tggcgcccgt gaaatgtttt
      181 ttgctaaaaa cgaaaagaaa gactttattg gtatgacttt tggcgaagtc ttaaaacgcg
      241 cagaaaacac cgcgattgct ctcatggaca tgggggttga acggggtgaa aaaataggat
      301 atttggccga caaccgcact gagtggatca tcggtgacat cgcctcgctt ttgacgggag
      361 gagtcaccgt gccgcgcggc accgatgtga cgatcgatga aattaagtac atcatggcgc
      421 atgccgagtg caaattttgc tttgttgaaa acgaagcgac tctcaaaaaa ttgcaatcgg
      481 tcattaagga taccaaagta gagaaaatta ttgtattaga tccaaaattc aaaggcacga
      541 acgagaacat ctttacgtta caacagctcg tcgacgcggg tgcgaaagtc cgcaaagaaa
      601 aattgcccgc gcttaaaaag cgtgttgaat ccatccagcc cgacgacctt ttcaccatta
      661 tctatacctc gggcacaacg gggcttccca aaggcgtgat gctctcgcac gggaacatgg
      721 tatacaatat caacgaagtg cccaaagcga ttggccttag acccgacgat agcatgcttt
      781 ctttattacc agtatggcat atttttgagc gtgccgtgga ttatggggct atctcaaaag
      841 gtattcctat ctattataca aatgtacgcg atgtgcgtga ggattttact aaagtaaagc
      901 cgacatttat gccgtctgcc ccccgtctgt gggagagtct ctatcaaggg attgaacaac
      961 ggatccaaaa atccgaaccc gcacgcaaga tgctctttga ggcggcgtac gctatcaaca
     1021 aagcgtataa aaaagcgacc gattaccttg cagggaacga gctcatgatg aagcccgaaa
     1081 ccgatttaga acgcctcacg cgcaccgcgc aggcgctttt cgttgtagca aacctgcact
     1141 ttcctgcacg ccttgccgac aaagtggttt ttggtaaaat ccgtgaagcg atggggggcc
     1201 gtttccgtgg cagtatttcc ggaggcggtg cgttgcccgc gcatgtggat gaatttttca
     1261 acgtcatcgg cgtaccggta tacgaaggct atggaatgac cgaatgtgca cccattattt
     1321 cggtacgtat cgaagggcgt attattcaag gcagcgtggg ctttgtgccc aatggcaccg
     1381 aggtcgctat cttaaacgac aaaggcgaac gcgtgaaaac gggagagaaa ggggttattc
     1441 atgtgcgagg gccgcaagtg atgaaaggct attataaaaa tcccgaagcg actgagaaga
     1501 caatcagtaa agacggatgg ctcaacacgg gtgacatcgg cttctttagt tttaataaca
     1561 ccctctcgat ccgtggtcgt gcaaaggaga ccatcgtcct cttgggtggt gagaacgtcg
     1621 aaccagtgcc gattgaaaat aaacttttag aagacgctca aattaaccag gtgattgtgg
     1681 tggggcaaga caagaaatca ctcactgcgc tcatct
//
LOCUS       DAOMPD010000555         1715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26375_length_1715_cov_2.354819, whole genome shotgun sequence.
ACCESSION   DAOMPD010000555 DAOMPD010000000
VERSION     DAOMPD010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26375_length_1715_cov_2.354819"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1228)
                     /locus_tag="PLY93_14115"
     CDS             complement(38..1228)
                     /locus_tag="PLY93_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70657.1"
                     /translation="MPVEELNTNYQDRMPSIAADGKFLFFSSDRPGGQGGDDIWVSQY
                     DDTNKKWGAPQNLKGINTAASEITPSIHSDGITLYYSSNKKGGVGGYDIYFTQSKARL
                     KNPDTEELFSGGWSPSLNLGKPFNSEFDDEYPTVTATGKRVYFTSNRPEGHGSFDVYW
                     ANVPDFARPLVRTRLKGSVFAGTSDRRVQAQLVLTLGERRIESTTDAEGNFDIPILNY
                     NETQIQAAAAHFRAYSYTLNTRNLKGEKTLDHTIRLVRDIQLPAKIQVHVQFVNAKNR
                     RISPRVKHRFLPHESVFNPDKVKKGSVVIPLLDIEKFNSEADAIRALEGTILEITAQL
                     KGEEPLTAKIKLQDLLDTWQNTIPKTIPLTLTILDTSATLSVTQKKLKRLPKRTKKVR
                     PQRR"
     gene            1209..1574
                     /locus_tag="PLY93_14120"
     CDS             1209..1574
                     /locus_tag="PLY93_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRP70658.1"
                     /translation="MSSSTGTGVNAVDLHGKTPQNIPQYAGSLWTTYQIQSGLFKGLG
                     FGGGPTYKSAVYVDIKNLMQVPGYVVADAVLFYKKEKWVMQVNFRNITNEVYYTSPSF
                     AGAMPGEPFNVIISAKASL"
BASE COUNT          399 a          355 c          454 g          507 t
ORIGIN      
        1 tttaacgtca acgctgtatt tagttttgta tttctactca ccgtcgttgt gggcgtactt
       61 ttttagtccg cttcggcaat cgttttagtt ttttctgagt tactgaaagc gttgcgcttg
      121 tatcgagtat tgtcagcgtt aaagggattg tttttggtat cgtgttttgc catgtatcga
      181 gaaggtcttg cagtttaatt tttgccgtga gaggctcttc gcctttgagt tgggcggtta
      241 tttcgagaat agtcccctcg agcgcacgaa tggcgtcggc ttcactgttg aatttttcta
      301 tatccagaag agggattact acgctccctt tttttacttt gtcggggtta aaaacacttt
      361 catgcggtaa aaaccggtgt ttgacgcgcg gagaaatccg gcggttcttg gcgttgacaa
      421 actgaacatg tacttgtatt ttagcgggaa gttggatgtc gcgtaccaaa cgaatggtat
      481 ggtcgagtgt tttttctcct tttagattgc gagtgttgag agtgtagcta taagcgcgga
      541 aatgcgctgc ggctgcttgg atttgtgttt cgttgtagtt taagatgggt atatcaaaat
      601 taccctcggc atcggttgtg gattcgatac gtcgctcgcc cagcgtcaaa acgagttgtg
      661 cttgcacgcg tctatcgctt gtgcccgcaa aaacactacc tttgaggcgc gtgcgtacga
      721 gggggcgcgc aaagtcgggt acgttcgccc aataaacatc gaatgagccg tgcccttcgg
      781 ggcggttgga ggtaaagtaa acccgcttac cggtggcggt gacggttgga tattcatcgt
      841 caaattcgga gttaaagggt ttgcctaagt tgagagaggg agaccaacct ccagaaaaaa
      901 gttcttcggt atcgggattt ttaagacgtg ctttcgattg agtgaaataa atgtcatagc
      961 cacccacgcc tccttttttg ttggatgaat aatagagagt aattccatcg ctatggatgg
     1021 agggagtgat ttcagaggcg gcggtgttga tacccttgag gttttgaggt gcgccccatt
     1081 ttttatttgt gtcgtcgtat tgacttaccc aaatatcatc accgccttga ccacctgggc
     1141 gatcggatga aaagaaaaga aacttaccgt cggcggcaat ggaaggcata cggtcttggt
     1201 aattggtatt gagttcttca acgggcacgg gagtgaacgc tgtcgacctt cacggcaaaa
     1261 caccgcaaaa cattccacag tacgcgggct cgctttggac gacgtaccaa atccagagtg
     1321 gtcttttcaa aggtttaggt tttggcggag gccccacgta taaatcggcg gtgtacgtcg
     1381 atattaaaaa cctgatgcaa gtaccaggtt acgttgtcgc cgacgcagtg cttttctata
     1441 aaaaagaaaa gtgggttatg caagtcaact tccgcaacat caccaacgaa gtgtattaca
     1501 cctcgccgtc ttttgcgggg gcaatgcccg gcgaaccctt taacgtgatt atttcggcga
     1561 aggcgtcgct atgacattaa atcgtatgag gctatacgcc acccgcgcta catacacttt
     1621 tgtcgtagaa tgaaaataac gaagtggcac cgttacgttg gtgttttttc tgccatcttt
     1681 ttggcagtaa gtgcgttaac aggaatttta tgggc
//
LOCUS       DAOMPD010000556         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26484_length_1709_cov_3.070738, whole genome shotgun sequence.
ACCESSION   DAOMPD010000556 DAOMPD010000000
VERSION     DAOMPD010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26484_length_1709_cov_3.070738"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..961
                     /locus_tag="PLY93_14125"
     CDS             209..961
                     /locus_tag="PLY93_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP70659.1"
                     /translation="MFKDKVAVITGSASPQGLGCATAEALAEKGCNVVIADINQENCD
                     KTAQMIAEKYGVKAIGVAANVTVKADNTALATAAVEKLGRLDIWVNNAGVLRDDLFMR
                     MSEEAWDLVQNVCLKGVFFGIQAATKVMMKAKYGKVINIASVAGLVGSPGQANYASAK
                     AGVIALTKTAAREYASRNICVNAIAPGYIHTGMTGVLPDKIKEELAKMAPLPHQGTPT
                     DIARAVRFLASPEADFITGVVLRVDGGAAIGL"
     gene            complement(1021..>1709)
                     /locus_tag="PLY93_14130"
     CDS             complement(1021..>1709)
                     /locus_tag="PLY93_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70660.1"
                     /translation="QNIKNRILLMGLSWRGETFMEMNPLDADATTQLLQVNLPLRLAI
                     LDFFSPHTFTLGATYIHDDALKVSLDTEFQWWSRFDVNVARKEYMNNNGISFKKMQNI
                     LLVRLGAETKPGKYIPKLREIPLLVRGGFSYTPAFTPDQTSYSNFLDNDKIAYSLGAS
                     YFLNSNSIVKAPVEIIFGFQHQIWLSRTSDKKGEVLTTTLPSSDPVNQPSYSYSAHVI
                     VVSLGALMKF"
BASE COUNT          492 a          392 c          369 g          456 t
ORIGIN      
        1 cccaatcacc ccttggccga aggacaaact cgttattttc atggatatat aactcctcgc
       61 tccccaccca caacttcatc gcccaccttc ccaactcgta caccctatct gcgcaacatg
      121 cataactctt tagactcctc ctttgtagcg tttgctacac agtcatgtat aaacttgacg
      181 agatgtttca ctattgtaaa tttaaccaat gttcaaagat aaagtagccg ttattacggg
      241 gtcggcctct cctcaaggct taggttgtgc gaccgcagaa gcgctcgccg aaaaaggatg
      301 caatgttgtt atcgccgaca ttaatcaaga aaactgcgat aagacagcgc aaatgattgc
      361 tgagaaatat ggcgtaaaag ctatcggtgt tgcggccaac gtcaccgtca aagcagacaa
      421 cactgcgctg gcaacagcgg cagttgaaaa attaggccgc cttgatattt gggtcaacaa
      481 cgctggcgta ttgcgcgacg acttgttcat gcgtatgtct gaagaagcgt gggatctcgt
      541 tcaaaatgtg tgccttaaag gggtcttttt tggtatccaa gctgcaacaa aagtaatgat
      601 gaaagccaag tatggtaaag tcattaatat tgcgtcagtt gcaggcctcg ttggtagccc
      661 tggtcaagcg aactacgctt cggctaaagc gggtgttatt gcgctcacta aaactgcagc
      721 acgcgaatac gcgtcacgca atatatgtgt caatgcaatc gctcctgggt atatccatac
      781 aggcatgact ggcgtattgc ccgacaaaat taaagaagag ttagcaaaaa tggctccact
      841 acctcaccaa ggcacaccta ccgacatcgc tcgtgcggta cgctttttag cttcccccga
      901 agcagacttt attaccggtg ttgtgctccg tgtagacggc ggtgcggcta tcggtttata
      961 ataacacgta acgtggctgg ctaatcagtc gcttgcgcgt tgattagaca gccaaaaaag
     1021 ttaaaatttc attaacgcgc ccaacgagac cacaattacg tgtgcactat acgaataact
     1081 gggttggttt accggatctg aggaaggtaa cgtcgtagta aggacttcac cctttttgtc
     1141 agaggtacga gacaaccaaa tttggtgctg aaagccaaag ataatttcaa caggtgcttt
     1201 tacaatcgaa tttgaattta agaagtacga cgctcccaac gaatacgcaa ttttatcatt
     1261 atctaaaaag tttgaatagc tcgtttgatc gggggtgaac gcaggggtgt atgaaaatcc
     1321 tccacggact aaaagaggaa tttctcttag tttcggaata tatttccccg gttttgtttc
     1381 tgctcccagg cgcaccaaaa gaatgttttg cattttctta aaggagatac cattgttatt
     1441 catatattct ttacgcgcaa cattgacatc aaagcggctc caccattgaa attcagtgtc
     1501 gagcgaaacc ttcaatgcat cgtcatggat ataagttgca ccaagagtga aagtatgcgg
     1561 tgaaaagaaa tctaaaatcg ctagccttaa aggtaaattc acctgtaata attgcgttgt
     1621 cgcatcggca tccaacggat tcatctccat aaatgtttcg ccacgccaac ttaatcccat
     1681 aagtaaaata cggtttttaa tgttttgac
//
LOCUS       DAOMPD010000557         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26542_length_1706_cov_2.281647, whole genome shotgun sequence.
ACCESSION   DAOMPD010000557 DAOMPD010000000
VERSION     DAOMPD010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26542_length_1706_cov_2.281647"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..661
                     /locus_tag="PLY93_14135"
     CDS             5..661
                     /locus_tag="PLY93_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70661.1"
                     /translation="MAFLLLIPTSISATLRLDYYYDQSGMFTKFSDWVPKRLHQWQPQ
                     FVEDFFLLYSLKQFSQQEELRRNIFFLKIALEKKFRHPREALCLIHNPQEYHKYRLLM
                     HMHINLQIMRSYLRLGSLFDKRHLYFYNLDFAPELKNSFAVAAGYYRESMPYWKKARA
                     LAIEASSYNFEIDMPGLESIRADILAGDIDYTVFTGNHLLRLKQKQQEVDAWLAQKKN
                     "
     gene            714..1292
                     /locus_tag="PLY93_14140"
     CDS             714..1292
                     /locus_tag="PLY93_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRP70662.1"
                     /translation="MRYFFVAIFIGLSILIPLGAQSVHSRLTDPQLLKRFDTLSHKLM
                     CTCGCNMPLHNCNHTGHCNAWPARDALDKLLLEGKTDAEILAGFRNGFGAIAETNEAF
                     AMARQNDYSYMLDKFRDGFGSQIMTRPETNFLAVFSILGGILCIGVVLLFLRSRTRKK
                     QATAVSTHPPIDDTRKNELLKRLSKDDYDDAL"
     gene            1580..1693
                     /locus_tag="PLY93_14145"
     CDS             1580..1693
                     /locus_tag="PLY93_14145"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRP70663.1"
                     /translation="MNIEALEKLSQIRKNGIINEAEFQAEKRKLYATHRNH"
BASE COUNT          474 a          382 c          349 g          501 t
ORIGIN      
        1 atggattgcg tttttgcttt taattccgac aagtatttcc gctaccctcc gtttggacta
       61 ttattatgat cagtctggaa tgtttactaa attttccgac tgggttccca aacggttaca
      121 tcaatggcag cctcaattcg tagaagattt ttttcttctt tatagtttaa agcaattttc
      181 gcaacaagag gagttacgcc gaaacatttt tttcttaaaa attgcgttag aaaaaaaatt
      241 ccgtcatccg cgcgaggcgc tctgtctcat ccacaacccg caagaatacc acaaatatcg
      301 actgctgatg cacatgcata tcaacttaca aattatgcgc tcatatttac gcttgggctc
      361 gctcttcgat aaacggcatc tttattttta caatttagat tttgcgcccg agttaaaaaa
      421 ttcgttcgcc gtcgctgcag gttattaccg tgagtcgatg ccttattgga aaaaagcgcg
      481 tgctttagcg atagaagcat cgtcgtataa tttcgagatt gacatgcctg gtcttgaaag
      541 cattcgcgcc gacattcttg ctggtgatat cgactacact gtttttaccg gtaaccatct
      601 tttgcgtctt aaacaaaagc aacaagaggt cgatgcatgg ttggcgcaga aaaaaaatta
      661 acaaagaaat aaaataaggt ttatcgcgtg tttctctttt tgtaaaagac ggtatgcgtt
      721 atttctttgt cgcaatcttc attggtcttt ctatcttaat tccccttggt gcacaaagtg
      781 tacacagcag attgaccgac ccccaacttc ttaaaaggtt cgataccctt tctcacaaac
      841 tcatgtgtac gtgcggttgt aatatgccgc tgcacaattg taaccacaca ggccattgca
      901 atgcttggcc tgcacgcgac gcgctcgaca aacttttatt ggaaggcaaa accgacgcag
      961 agattctggc gggcttccgt aatggttttg gtgcgattgc cgaaacaaac gaagcctttg
     1021 cgatggcacg ccaaaacgat tattcctata tgttggataa atttcgcgac ggctttggct
     1081 cacaaattat gacacggcct gaaaccaatt ttttggctgt tttttcaatt ttgggtggca
     1141 ttttgtgtat tggtgttgtt cttctttttc tccgcagccg cacacgtaaa aaacaagcca
     1201 cagcggtatc tacacaccct cccatcgacg atacgcgcaa gaatgaactt ctgaaacgac
     1261 tctccaaaga cgactacgac gacgcattgt agaggagcca aaagcagtcc agccttaagg
     1321 atagttttct tcgagcttca cgctggcttt tgagcgcccc aacacgtaca ccgatggtgg
     1381 agcaaacttt tttgcctctt tgtttatgat tttaactctg tgtctcggtg cttgcaaaga
     1441 aaagcagatg tatttgcaca tgcccgatgg ccaagttaag acggttaaaa cctcagaatg
     1501 cgaaacatat gtaaagaatg ccgcagctat gggcgttact gctatttgca aggctcagcc
     1561 attctaatcg tagggtcgca tgaatattga agcattagaa aagctgtctc aaatccgcaa
     1621 gaatggtatc atcaatgaag ccgaatttca agctgaaaaa agaaaattat atgcaaccca
     1681 cagaaatcac tgacactttt ggggtc
//
LOCUS       DAOMPD010000558         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26616_length_1703_cov_2.305218, whole genome shotgun sequence.
ACCESSION   DAOMPD010000558 DAOMPD010000000
VERSION     DAOMPD010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26616_length_1703_cov_2.305218"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..569)
                     /locus_tag="PLY93_14150"
     CDS             complement(33..569)
                     /locus_tag="PLY93_14150"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRP70664.1"
                     /translation="MTEIAKKIDACVRNVPDFPKPGVMFKDITPILQNAQVFKETIQT
                     MAAQLKNENVRYVAGIESRGFVFASALALELGIGLVLLRKPGKLPWRTMKATYQLEYG
                     TDAIEVHEDAVEKGDVITIVDDLLATGGTAAAAASLLREAGADVKKILFVIELDFLKG
                     RAKLNDAGFAHVESLVHY"
     gene            complement(572..1561)
                     /gene="rfaD"
                     /locus_tag="PLY93_14155"
     CDS             complement(572..1561)
                     /gene="rfaD"
                     /locus_tag="PLY93_14155"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRP70665.1"
                     /translation="MIIVTGGAGFIGSAVVAKLNAMGEQNIVIVDNLTDSEKWKNLVA
                     LQYSHYIHKSHFLAALQKGEIKNVKTIIHMGACSATTETDGDYLMANNYAYTRFLAEY
                     ALENGMRFIYASSAATYGGIESNFNDDDATSLTLRPINRYGYSKQIFDAIAIKNGWTS
                     RIVGLKFFNVYGPNEYHKADMSSVIYKAFHQINENGSLKLFKSYRNEFGDGEQKRDFV
                     YIKDCVAVIAELVQKPHIHGIYNLGTGKARSWNDLAAAVFNAMGREKKIEYIEMPTKL
                     QGSYQYFTEANMDRLNASGIPLLRTSLEEGVKDYVQNYLIPGSLKLQDQPLEG"
BASE COUNT          427 a          414 c          382 g          480 t
ORIGIN      
        1 cgtacacccc ggtgagggta ttttgagcgg catcaatagt ggacaagact ttcgacgtga
       61 gcgaagcccg catcgttgag tttggcgcgg cctttcaaga aatcaagttc aatgacaaaa
      121 agaatttttt tcacgtcagc gcccgcctcg cgcaacaagc tcgccgctgc cgccgccgta
      181 ccgccggtcg ccaaaagatc gtcgacgatc gtgatgacgt cccctttttc aaccgcgtct
      241 tcgtgtactt cgattgcgtc tgtgccatat tccaactggt acgttgcttt cattgtacgc
      301 caaggcaatt taccgggttt acgcaaaagc accaagccga tacccaactc caacgccaat
      361 gcgctcgcaa acacaaaacc acgcgattca attcccgcga catagcgtac gttttcattt
      421 tttaattgcg ccgccatcgt ttggatggtt tctttgaaaa cttgtgcgtt ttgtaaaatg
      481 ggcgtaatgt ccttaaacat aaccccgggt ttagggaagt cggggacgtt acgcacgcat
      541 gcgtcgattt tttttgcaat ttcagtcata gttatccttc aagtggttga tcttgcagtt
      601 tgagactgcc ggggattaaa taattttgca cataatcttt cacgccttct tcaagcgatg
      661 tgcgcagaag aggaatgccg ctggcgttta agcgatccat attggcttcg gtgaagtatt
      721 ggtagctgcc ttgtaacttc gttggcattt ctatatattc aatttttttc tcacgcccca
      781 tcgcgttaaa aacagcggcg gctaagtcgt tccaactgcg cgctttgcca gtgcccaagt
      841 tgtaaatccc gtggatgtgt ggcttttgaa cgagctcggc aatcaccgcc acacaatctt
      901 tgatatacac aaaatcgcgt ttttgttcgc catcgccaaa ttcgttgcgg tatgatttaa
      961 aaagcttcaa cgaaccattt tcgttaatct gatggaacgc cttgtagatg acgcttgaca
     1021 tatccgcttt gtggtattcg ttggggccgt atacgttaaa aaatttgagt cccacgatac
     1081 gcgatgtcca tccatttttg atagcaattg cgtcaaaaat ttgttttgaa taaccatagc
     1141 ggttaatcgg ccttaatgtc aaactcgtgg cgtcgtcgtc gttaaagttc gactcgatgc
     1201 cgccgtatgt ggctgccgag gaagcataaa taaaacgcat accgttttcg agcgcatact
     1261 ccgccaaaaa gcgtgtgtac gcatagttat tcgccattaa ataatcgccg tcggtttcgg
     1321 tcgtcgccga acacgcgccc atgtggataa tcgtttttac gtttttaatc tcaccctttt
     1381 ggagcgccgc caaaaagtgg gatttatgaa tgtaatggct atattggagt gcaactaaat
     1441 ttttccactt ttccgaatcg gttaagttat cgacaatcac aatgttttgc tcgcccattg
     1501 cgttcaattt tgccacaacg gcgctaccga tgaaccctgc cccacctgtg acaataatca
     1561 tggggaactt ttagaagcag agaacagcat ggaaagcaca aacagagatg ggaacaaaaa
     1621 tctgcaaaaa aattgtctca gcgaattggc gcaggttcac aggctcacag atggaaagac
     1681 tgacttttca ttgccgcgct ctc
//
LOCUS       DAOMPD010000559         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26703_length_1699_cov_2.521898, whole genome shotgun sequence.
ACCESSION   DAOMPD010000559 DAOMPD010000000
VERSION     DAOMPD010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26703_length_1699_cov_2.521898"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLY93_14160"
     CDS             complement(<1..441)
                     /locus_tag="PLY93_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRP70666.1"
                     /translation="MKIHEYQGKEIFRKYKIAVPNGIVTTKADEAAAIFTKLGKPVSV
                     VKAQIHAGGRGKGGGVKLAKSAEEAKAHASAILGMMLKTAQTGPEGQKVNTILIEEGS
                     DIIKEYYVGITLDRRSSKPVMMVSTEGGMEIEEVAHKTPEKILKE"
     gene            743..>1699
                     /locus_tag="PLY93_14165"
     CDS             743..>1699
                     /locus_tag="PLY93_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRP70667.1"
                     /translation="MVNVHIQIDNDEGDFSLDTTFAEVIEKLAPKKRKAVLGVEVDKK
                     SYDLSRSLSTIKSLSAVETSTPPTLPIKLLTFDDEAGKEIFWHSSAHALAQALKRLYP
                     KIQLEDGPVVKNGPGYFFYDVLLDEKINEDAFEKIENEIQKVIRENHPIHRTVMERKD
                     AVSKFKAMGEDLKVGIIEGLPTDAEISIYEQGEFADLCRGPHVPSTGKLGVFKLTTVA
                     GAYIGGKAENQMLQRIYAVSFPDKQMLKEHFRLLEEAKKRDHRRLGQELELFTTSEDI
                     GPGLILWLPRGNIIKEELEAWAKDTEAAHGYERVTTPIVTKEK"
BASE COUNT          456 a          408 c          402 g          433 t
ORIGIN      
        1 ttctttgagg attttttctg gtgtcttgtg tgccacctct tcaatttcca tgccgccctc
       61 ggttgaaacc atcatcaccg gttttgaact gcggcggtcg agggtgattc ccacgtagta
      121 ttctttgata atatccgatc cttcttcgat gaggattgta ttgacttttt gcccttcggg
      181 gccggtttgt gcggttttga gcatcatgcc caaaatggca ctggcatgcg ctttggcttc
      241 ctcggccgat ttagcgagtt tcactccgcc gcctttaccg cgccccccag cgtgaatttg
      301 tgctttaacg actgagacgg gtttacctaa cttggtaaag atagcagcgg cttcgtctgc
      361 ttttgtcgtt acaatcccgt tgggtacggc aattttatat ttacgaaaaa tttctttgcc
      421 ttgatattca tgaatcttca tggtggattg agactaagcg gaaaataggg ggtcaagaag
      481 aaggtgataa tttcagagcc tgtgcataca tttcgcatta tgaaaactca attcaaccta
      541 ctgttttgga tggcactcgt ggctgcaaac catagaaatg tccattccaa tgttttttag
      601 cggggcgcac gtagatacca taataacgga tagatttctc atgtcggtca aggaagaaaa
      661 agagtatgcg cgtcataggc ttttaagtgc aaaccttgct attcttactt tatcgcctgt
      721 ttcaaccctt agttttgcac acatggtaaa cgtacacatc caaatcgaca acgacgaggg
      781 ggatttctct ctcgatacca cgtttgcaga agtgattgaa aaactcgcac ccaaaaaacg
      841 caaagcggtg ttgggcgttg aagtcgataa aaaaagctac gacttatcgc gctctctttc
      901 aaccataaaa tcgctgagcg cagtcgaaac gagtacgcca ccaacactac ccattaaatt
      961 gctcactttc gacgatgagg cgggcaaaga aattttttgg cattcgagcg cacacgctct
     1021 tgcacaggca ctcaagagat tataccctaa aatccaactc gaagacgggc cggtcgtcaa
     1081 aaatggccct gggtattttt tttacgatgt tctcctcgac gaaaaaatta acgaagacgc
     1141 gttcgagaaa attgaaaacg aaattcaaaa agtcattcgc gagaaccatc ccatccaccg
     1201 cacggtgatg gagcgtaaag acgcggtttc aaaattcaag gcgatgggcg aagacttgaa
     1261 agtcggcatt attgagggcc tccccaccga cgctgaaatc agcatctatg aacaaggcga
     1321 gtttgccgat ctttgtcgtg ggccacacgt cccttccacc ggtaagctcg gtgtctttaa
     1381 gctcaccact gtggcgggcg cgtacatcgg cggcaaggca gaaaaccaaa tgctacagcg
     1441 catctatgcg gtgagctttc ccgataagca aatgctcaaa gagcatttta gactcctcga
     1501 agaggccaaa aagcgcgacc accgccgctt gggtcaagag ttggaacttt tcaccacgag
     1561 cgaagatatt gggccggggt taatcttatg gctgccgcgc ggcaacatta ttaaagaaga
     1621 actcgaagcg tgggcgaaag ataccgaagc ggcacacggg tatgaacgcg tcacgacgcc
     1681 catcgtcacc aaagaaaaa
//
LOCUS       DAOMPD010000560         1692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_26837_length_1692_cov_2.648748, whole genome shotgun sequence.
ACCESSION   DAOMPD010000560 DAOMPD010000000
VERSION     DAOMPD010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26837_length_1692_cov_2.648748"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1692
                     /locus_tag="PLY93_14170"
     CDS             <1..>1692
                     /locus_tag="PLY93_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRP70668.1"
                     /translation="RAVFFGESDDSRLVGDFPLNPADSVRMWALTRVVDLHGNFYEVR
                     YRQDSGEIYPAKIIYTQNTNGGIARFRTVEFEYDENAYPNAAYSYASSSPTRTRWRLR
                     DIVVQVNVVTFLGITIPFTGDLVRRYHLEYETSAASGLSRLISIQEFGSDNSTALSPH
                     LFEWHTSPQDFTQTFTTPADNSGWSKNYGDTIVGDFNGDGKSDIVLQGQYDTYTNLHL
                     SSGDTFKDTAIPMDTFTNGWRLFDFSGEESLKVTALGDIDGDGRIDIVSSFAAQNTTT
                     NIIDRYVFSPDTTDNIGTRLIHLPKNNTSMYDATDRNIHVADFDGDGRSDILLQANTT
                     AQASYIIRSTGIATNLTFQRIELDIAISGTDTLLIADFNGDARNDILQCTKNEALGHS
                     TVKLHLSSYDATIAPVGAPIRFKTQNLPMLLPLDCRATLGDFNGDGKLDVISSTGGHK
                     LYLGNGFTFADAVVIDTQSIALTGVHTAAESTLLAGDFNGDGRDDLFVYSTSSLAGAH
                     IAYSYGENFSGHGGLTSFPWQSTDAKWRTALNDVTLADINGDGRIDIVVSEKRPQG"
BASE COUNT          420 a          411 c          424 g          437 t
ORIGIN      
        1 cgtgcagttt ttttcggaga gagcgacgac tcgcgtcttg tcggcgactt tcccttgaac
       61 cccgccgatt cggtgcgcat gtgggcattg acgcgtgttg tcgatttgca cggcaatttt
      121 tatgaagtgc gctatcgtca agatagtggt gagatatacc ctgcaaaaat tatctatacg
      181 caaaacacaa acggcggcat cgcgcgtttt cgcacggttg aatttgagta cgatgaaaat
      241 gcatacccca acgccgcgta cagttatgcg tcaagttcgc ctacgcgtac tcggtggcgg
      301 ttacgtgata ttgtcgttca agtaaacgtc gttacatttt tgggcattac gattcctttc
      361 accggcgatt tagtgcggcg gtatcattta gaatatgaaa ccagcgcggc aagcgggctt
      421 agccgcttga tttcaattca agaatttggg agcgataatt cgactgcact atcgccgcac
      481 ttatttgaat ggcatacatc gccgcaagac tttacacaga cttttacaac gcctgcggat
      541 aatagcggtt ggagtaaaaa ttacggcgac acaattgtcg gcgatttcaa tggcgacggc
      601 aagagtgata ttgttttgca ggggcaatac gatacgtata caaatttgca tctttcaagc
      661 ggtgatactt ttaaggatac cgctataccg atggatacct tcaccaacgg ctggaggctt
      721 tttgattttt caggcgaaga gtcgcttaaa gtaaccgcat tgggcgatat tgacggcgac
      781 gggcgtatag atattgtgag tagctttgcg gcacaaaaca ctacgacgaa tataattgac
      841 cggtacgtat tttcacccga cacaaccgat aacattggta cgcgcttgat acatttaccc
      901 aagaataata cgagtatgta cgacgccacc gaccgcaaca tacatgtcgc cgattttgac
      961 ggcgacggta ggagcgatat tttgctgcaa gcaaacacga cggcgcaagc atcgtatatt
     1021 atacgctcca ccggtatcgc cacaaacctc acctttcaac gtattgaact cgacatcgcg
     1081 atttctggta cagacacttt gttgattgcc gattttaacg gggatgcacg aaacgacatt
     1141 ctacagtgta cgaaaaacga ggcgttgggt cactccaccg tgaagttgca tttatcttcg
     1201 tacgatgcga ctattgcgcc tgtgggtgcg ccgattcgtt tcaaaaccca aaacctgcca
     1261 atgcttttgc ctctcgattg ccgtgcaacg ttaggcgact tcaacggcga cggcaagctc
     1321 gacgtcattt catcgacggg cgggcacaag ttgtatctcg gcaacggctt tacctttgcc
     1381 gacgccgtcg tcatagatac gcaatcgatt gcgctaacag gcgtgcatac cgccgccgag
     1441 tcgactctgc ttgccggtga ttttaacggc gacgggcgcg acgatctctt tgtgtatagc
     1501 acttcgtcgc ttgcgggtgc gcacatcgcg tattcgtacg gcgaaaattt ttcgggtcac
     1561 ggcggattga cttcttttcc ttggcaatcg accgatgcaa aatggcgtac tgcactcaac
     1621 gacgtgactc ttgccgatat aaacggcgac gggcgtatcg atattgtcgt aagtgaaaaa
     1681 agaccgcaag gc
//
LOCUS       DAOMPD010000561         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27144_length_1676_cov_2.185688, whole genome shotgun sequence.
ACCESSION   DAOMPD010000561 DAOMPD010000000
VERSION     DAOMPD010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27144_length_1676_cov_2.185688"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..802)
                     /locus_tag="PLY93_14175"
     CDS             complement(161..802)
                     /locus_tag="PLY93_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70669.1"
                     /translation="MLSSSKTITFFLLFFAFGCASQWNTVRTNHDEKANQTIITLESN
                     LYVNVDGNRMREMLKGERRLTISFKRTQNAQQKSPVALKLQLYPTDAKPHLQFWIGCI
                     NPKTKFPLVLIKSEPLEETVTVLDVDDRDNPNLSPDTLSQTSDINFGKPVTASAVNRR
                     WVLYTLNMSDELALLKAVDGCSKAQIEFQLGDKRAVAEAGEKEIAAWKAYKAT"
     gene            complement(786..1016)
                     /locus_tag="PLY93_14180"
     CDS             complement(786..1016)
                     /locus_tag="PLY93_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HRP70670.1"
                     /translation="MQLIVHAKAGNKNPGIERISGTEWIVRVRERALDGKANAALKAA
                     IAHELGIAQSCVQLVSGEKSKLKRFKIDAIVV"
     gene            complement(1022..>1676)
                     /locus_tag="PLY93_14185"
     CDS             complement(1022..>1676)
                     /locus_tag="PLY93_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP70671.1"
                     /translation="TVKEFSFKVGDILFLASDGRDDIVIKEGDSNTTRVINEDEKAFL
                     NVILRGEGDLQKTVNNLRQTGGLSDDLSIIRIEILGFNSEAIRKEKKSIKETVRERLV
                     EIKDLIRKKNYAGAAHALQHVSTELSIENVPEYYILLSRIQLNDGQLQMARQSLEKAL
                     QLGHEVAPAYKSLGNIFYKLKMLPEAIDAWEHALKLNPDDKALSHLLRDIEAQKTTT"
BASE COUNT          376 a          410 c          394 g          496 t
ORIGIN      
        1 gcgctgatgt tgagtcccga cgctccggcg attaaaagtt tgtgagcggc gccggtgttc
       61 ttttcaaaaa gtgaaacgcg caccccccgc tgtgcaagca cgctcgccgc catgagacct
      121 gcggggcctg tgcccacaat cgcaacgcgc ataaattatt ttacgtggct ttgtacgctt
      181 tccacgcggc gatttctttt tctcccgctt ctgccacggc gcgcttgtcg cccaattgaa
      241 attcaatctg cgctttggag caaccgtcaa ccgctttgag cagcgccagt tcatcgctca
      301 tgttcaaagt atataaaacc caacggcgat tgaccgcgct ggcggtgacg ggtttaccga
      361 aattgatgtc gctcgtttgg gatagggtat cgggggataa attggggtta tcgcggtcgt
      421 cgacatcgag cacggtgacc gtttcctcca acggttcgct ttttataagt accaaaggaa
      481 attttgtttt aggatttatg cacccaatcc aaaattgcaa gtgcggtttt gcatcggtgg
      541 gataaagttg aagctttaat gccacaggag atttttgttg cgcgttttgg gttcgcttaa
      601 aagaaatggt tagtcgccgc tcgcctttca gcatttcgcg catacgattg ccgtcgacgt
      661 tcacgtagag attgctttca agcgtgatga tggtttggtt tgccttttcg tcatggtttg
      721 tacgcactgt attccactgc gatgcacaac cgaatgcaaa aaaaagaaga aaaaaagtta
      781 ttgttttaga cgacgatagc atcgatttta aacctcttga gcttactttt ctcccccgag
      841 acaagctgca cacacgattg tgcaataccc aactcgtgag caattgctgc ttttaatgcg
      901 gcatttgctt taccatcgag cgctcgctcg cgcacacgga ctatccactc tgtgccgctg
      961 atgcgttcaa tgccagggtt tttgtttccg gcctttgcat gcacgatgag ctgcacaaaa
     1021 attacgttgt cgttttttgc gcttcgatgt cgcgcagcaa atgactcaac gctttgtcgt
     1081 cggggttgag cttgagcgcg tgctcccacg cgtcgatagc ttcgggcaac atttttagtt
     1141 tatagaaaat gttgccgaga gacttgtacg cgggcgcgac ttcatgcccc aactgtaatg
     1201 ctttttctaa actttgccgc gccatttgca actgaccatc gttcagttga atgcggctca
     1261 aaaggatata atactcgggt acgttttcga tactcaactc ggttgaaacg tgttgcaatg
     1321 catgggcggc gcccgcgtaa ttttttttgc ggattaaatc ttttatctcc acgagacgtt
     1381 cacgcacggt ttcttttatc gatttttttt ctttgcgtat cgcttcgcta ttaaaaccta
     1441 aaatctcgat acgaataatc gacaagtcgt cggacaaccc cccggtttgg cgtagattgt
     1501 ttaccgtctt ttgtaaatcg ccttcaccac ggagaataac attcaagaaa gctttttcgt
     1561 cttcgttgat cacgcgcgtt gtgttgctat cgccttcttt gatgacaatg tcgtcgcgtc
     1621 cgtctgaggc gagaaataaa atatccccca ccttaaatga aaattcttta accgtg
//
LOCUS       DAOMPD010000562         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27149_length_1676_cov_2.031462, whole genome shotgun sequence.
ACCESSION   DAOMPD010000562 DAOMPD010000000
VERSION     DAOMPD010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27149_length_1676_cov_2.031462"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>620
                     /locus_tag="PLY93_14190"
     CDS             <1..>620
                     /locus_tag="PLY93_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRP70672.1"
                     /translation="ALAELVDLPHHPQVLLKVAQGMKSEKRIVYVKRGTTQKEYLKNV
                     EGAENKRPQMRGAYQRTTSALFEISPRVVKENRKGDLERLRLRYMLPGDFEKEIIPLK
                     KVLDKRVKEEIKNGKRRKVEGRYIFTIDGADAKDFDDAIAVVETQEGFELDVHIADVG
                     FFVPPESLLFSEALRRGNSYYLSGSVIPMLPEILSNEFCSLKPRTTR"
     assembly_gap    621..630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            641..>1676
                     /locus_tag="PLY93_14195"
     CDS             641..>1676
                     /locus_tag="PLY93_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRP70673.1"
                     /translation="MRFDKKGKMIGYEFFKSTIYIDKRYTYDEAHENLKKNKSPLKSA
                     MRLAKILIEARDKAGRVELNFNEDKILYDKNGRFVGITKSERLDSHRLVEECMLSANQ
                     AVAAYAIKFNLPILHRNHESMPQEKLERLNRYLEKYVPRLKLKSTEQNELNRVLNDAA
                     VQKVKDVFQFLLLRSFMQANYAPEPKGHWGLAFTHYAHFTSPIRRVADLVTHIQLDAH
                     LARKKFPFKNDLLDHYGREASRLERIAFEAEKSDKKLLAIRALQNRVGETFTGWLSSF
                     SSDRLFITLNEFPAEGEIAASAVDKRGEIRVVDDFSIFSGRLQKTLSLGDTLKVRLVK
                     ADALPIALKFE"
BASE COUNT          490 a          381 c          395 g          400 t
ORIGIN      
        1 gctttggccg agctcgtcga tctaccccat caccctcaag tgttgcttaa agttgcacag
       61 gggatgaaaa gtgaaaaacg tatcgtttat gtaaagcgtg gcacgactca aaaagaatac
      121 ttaaaaaacg tagaaggcgc agaaaataaa aggccgcaga tgcgcggtgc ataccagcgc
      181 accacttcag ccctttttga aatttcgccg cgcgtggtga aggaaaatcg caaaggcgat
      241 ttagaaaggc tgcggctgcg ctatatgttg cctggcgatt ttgagaaaga aattataccg
      301 ctgaaaaaag ttttggataa gcgtgtcaaa gaagaaataa aaaacggaaa gcggcgcaaa
      361 gtcgaggggc gttatatatt tactatcgat ggcgccgatg ccaaagattt cgacgatgcc
      421 attgccgtcg ttgaaactca agaaggcttt gaactcgatg tgcatatcgc cgatgtggga
      481 ttttttgtac cgcccgagag tttacttttt agcgaagcgt tgcggcgtgg gaatagttac
      541 tatctttccg gttcggtaat ccccatgctg ccagaaattc tctcgaatga attttgcagt
      601 ctaaaaccta gaaccacgcg nnnnnnnnnn tacgtcgcga atgcgctttg ataaaaaagg
      661 caagatgatc ggctatgaat tttttaagtc gacgatttat atcgacaaac gctatacata
      721 cgacgaagcg catgaaaatt tgaaaaaaaa caaatctccc ttgaaaagcg caatgcgcct
      781 tgccaaaatt ttaatcgaag cacgcgacaa agccggacgg gtggagctca actttaacga
      841 ggataaaatt ttatacgaca aaaacggccg ttttgttggg ataacaaaat cagaacgcct
      901 cgattcacac cgcctcgtcg aagagtgcat gctgagtgca aaccaagcgg ttgccgcgta
      961 cgcgattaaa tttaatttac caattttaca ccgcaaccac gagagcatgc cgcaagaaaa
     1021 actcgaacgc ctgaaccgat atttagaaaa atatgtccca cgcctcaaac tcaagagcac
     1081 cgaacaaaat gagttgaacc gtgtgctaaa tgacgctgcg gtacaaaaag tgaaagatgt
     1141 ttttcaattt cttttattgc gttcttttat gcaggcaaac tatgctcccg aacccaaagg
     1201 ccactggggg ttagctttca cgcactacgc acacttcact tcgcccatcc gccgcgttgc
     1261 cgaccttgtg acgcacatcc agttggatgc acatttggcg cgcaaaaaat ttccgttcaa
     1321 aaacgaccta ctcgaccact atggccgtga agcgtcacgc ttggagcgca tcgcttttga
     1381 agcggagaaa tcggataaga aactgttggc catccgtgca ttacaaaata gagtcggcga
     1441 gacgttcacc ggttggctga gtagtttttc cagcgatcgg ctttttatca ctctcaatga
     1501 atttcccgcc gagggggaaa tcgccgccag cgcagtggat aaacgcggtg aaatccgcgt
     1561 cgtcgacgac ttcagtattt tttcggggcg cttacagaaa acattgagtt tgggcgatac
     1621 attaaaagta cgcttagtga aggccgacgc gcttcctatt gcgctcaagt ttgagt
//
LOCUS       DAOMPD010000563         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27171_length_1675_cov_1.993210, whole genome shotgun sequence.
ACCESSION   DAOMPD010000563 DAOMPD010000000
VERSION     DAOMPD010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27171_length_1675_cov_1.993210"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>502
                     /gene="secA"
                     /locus_tag="PLY93_14200"
     CDS             <1..>502
                     /gene="secA"
                     /locus_tag="PLY93_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758870.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRP70674.1"
                     /translation="MRPLYNFHGLSTGVIKANMGHAQRKEAYAADITYGTNNEYGFDY
                     LRDNMVDHKAYRVQRDHYFAIVDEVDSILIDEARTPLIISGPADQDAGEYVRVNKIIP
                     SLQDKVDYDVDEKARTVLLTELGVHKVEKLLNLDNLYAPQNVELVHHVHQCLKAHKLF
                     QRDVDYV"
     assembly_gap    503..531
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <532..>1675
                     /gene="secA"
                     /locus_tag="PLY93_14205"
     CDS             <532..>1675
                     /gene="secA"
                     /locus_tag="PLY93_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804165.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRP70675.1"
                     /translation="IIIVDEFTGRLMEGRRYSDGLHQAIEAKERVEVKQENQTLATIT
                     FQNFFRMYEKLAGMTGTADTEAEEFRKIYKLDVTVIPPNKKLIRKDAADKVYRTQREK
                     FEAIAEEVKERHLHGQPVLLGTVSIENSEKLSKLLNVKGVPHSVLNAKHHDQEAQIIK
                     DAGQRGHITIATNMAGRGTDIILGEGVRELGGLHIIGSERHESRRIDNQLRGRSGRQG
                     DPGSSRFYLSLEDELMRIFGSDRLGPIMQRFGMQEGEALEHRMVTNAIERAQKRVESH
                     HFEMRKHLIDYDDVMNMQRKYIYMVRNEILDNADIKSLIREFVDDAISGRVEQMLPGR
                     KVSEYNIDALKEYLSTVYQIQYENLEDVVRDSSKDEFIDDVRKKAFA"
BASE COUNT          470 a          437 c          406 g          333 t
ORIGIN      
        1 ggatgcgacc gctctataat ttccacggcc tttccaccgg cgtcatcaaa gccaacatgg
       61 ggcacgcaca gcgcaaagaa gcgtacgccg ccgacatcac ttacggcacc aacaacgaat
      121 acggtttcga ttatctgcgc gacaacatgg tcgatcacaa agcgtaccga gtgcagcgcg
      181 accactactt cgcgattgtg gacgaagtgg actcgattct catcgacgaa gccagaacac
      241 ctctcatcat ctcaggcccc gccgatcaag acgcgggcga atacgtgcgc gtgaataaaa
      301 ttattccttc actacaagac aaagtcgatt acgacgtcga tgaaaaagca cgcactgtgc
      361 ttttaaccga attgggagtg cataaagttg aaaaattatt gaacctcgac aatttatacg
      421 ctccccaaaa cgtagagctt gttcaccatg tgcatcaatg cctcaaagcg cataaacttt
      481 ttcagcgcga cgtggattac gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattattatc
      541 gtcgacgagt tcacagggcg cctcatggaa ggccgccgct atagcgacgg gcttcaccaa
      601 gcgatcgagg ccaaagagcg tgtcgaagtg aagcaagaaa accaaactct cgccacgatt
      661 acctttcaga atttttttcg tatgtacgaa aaactcgcgg gtatgacggg tactgccgac
      721 accgaagccg aagagtttcg taaaatatat aaactcgacg tgacggtgat tcctcccaac
      781 aaaaaactca tccgcaaaga cgccgccgac aaagtgtacc gcacgcagcg tgaaaaattc
      841 gaggcgattg ccgaagaagt gaaagaacgc cacttacacg gccagccggt tcttttgggc
      901 actgtgtcga ttgagaactc agaaaaactc tccaaacttt tgaacgtaaa aggcgtgccg
      961 catagcgttc tcaacgcaaa acaccacgat caagaagcgc aaatcatcaa agacgcagga
     1021 caacgcggac atatcaccat cgccaccaac atggcagggc gcggtaccga catcatcctc
     1081 ggtgaaggcg tgcgcgagtt gggtggtctt cacatcattg gttctgagcg ccacgagtcg
     1141 cggcgtatcg acaaccagtt acgcgggcgt tcggggcgcc agggcgaccc gggtagttcg
     1201 cgtttttact tgtcgctcga agacgagctc atgcgcattt tcggttccga ccgtttgggc
     1261 cccatcatgc aacgcttcgg catgcaagaa ggcgaagcgt tggagcaccg catggtgacg
     1321 aacgccatcg agcgcgcgca aaaacgggtc gagtcacacc actttgaaat gcgtaagcac
     1381 ctcatcgact acgacgacgt gatgaacatg cagcgcaagt acatctatat ggtgcgcaat
     1441 gaaattttag acaatgccga cattaaaagt ctcatccgtg agtttgttga cgacgccatc
     1501 agtgggcgcg tcgagcagat gcttccaggc cgcaaagttt cagagtacaa catcgacgca
     1561 cttaaagaat acttgagcac tgtctatcaa attcaatatg agaatttaga agacgtggtg
     1621 cgcgattcaa gcaaagacga gttcatcgac gacgtacgca aaaaagcatt cgccg
//
LOCUS       DAOMPD010000564         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27424_length_1662_cov_2.476665, whole genome shotgun sequence.
ACCESSION   DAOMPD010000564 DAOMPD010000000
VERSION     DAOMPD010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27424_length_1662_cov_2.476665"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLY93_14210"
     CDS             complement(<1..570)
                     /locus_tag="PLY93_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP70676.1"
                     /translation="MSAAALATEQDPLLCIKDLHLTFHVGKKDAHTLRGIDLILKRGE
                     ILALVGESGSGKSITGLSILKLHSGNTTMTGSIFFKKEKISLLNDEDMRAYRGKKIAM
                     IFQEPMTALNPVFTIGYQLGEVLALHKNLKGSAIDDAAVQLLEEVGIPEPRLRLKSYP
                     FQLSGGMRQRVLIAMALAACGSGSDSTPES"
     gene            849..1526
                     /locus_tag="PLY93_14215"
     CDS             849..1526
                     /locus_tag="PLY93_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRP70677.1"
                     /translation="MRRSNAVMKAIKKNRIFHVALTRCCFLPFFYPMDSPQSRDVSTD
                     ALTIVALGDSITWGYPNGHSWTEMVQEDTGIRIINRGVNGATLHNMLNRMPTDVLPVK
                     PSICIIMGGTNDANQGRTTKDMMADIQKMANELMAHDIMPVVALPIPIVWQKPEEKLQ
                     DLRERIISSAFLTLDFALDFDLPRAEFKQLLPDGLHPTAEGKKMMAERLKKEMPRLLA
                     AYAKFRK"
BASE COUNT          428 a          382 c          400 g          452 t
ORIGIN      
        1 ggattccggt gtcgaatccg agcctgatcc gcacgctgcc agtgccattg cgatgagtac
       61 acgttgccgc atccctcccg aaagttgaaa cgggtaactc ttgaggcgca gccgcggttc
      121 ggggatgccc acttcttcga gtagttgtac cgctgcatcg tcgatagcac tcccttttag
      181 atttttatgt aatgcaagca cttcgcccag ttggtagccg atggtaaaaa cggggttgag
      241 tgcggtcatc ggttcttgaa aaatcattgc aatttttttg ccacggtacg cacgcatgtc
      301 ttcgtcgttt aagagtgaaa ttttttcttt cttaaaaaaa atactccccg tcattgtagt
      361 attgccactg tgcaacttta agatactcaa accggtgatc gacttccccg aacccgattc
      421 accgacgagg gctaagattt caccgcgttt tagtattaaa tcaatcccac gcaacgtgtg
      481 cgcgtctttt tttcctacat ggaacgtgag gtgcaaatct ttaatgcaaa gcagggggtc
      541 ttgttctgtt gccagtgcgg cggcgctcat ttattctacg atttgttttt ggtttaacgc
      601 gtaaagcgaa ggagtaagga gtctgtgcgc caactcgcgc agagcctgtg tttcccaggc
      661 gacgttaaat ttatataaac ttcaacccaa cgcttgccga cctttttaaa gaggaaccga
      721 agagtctgtg cgacaactca tgcagtgtgt acaaaaattt gcaaaaaaat tgcctcagcg
      781 agttggtgca caagctccta agaacgagtt ctccttgacg cgccgtgcta aatactacaa
      841 atggcagaat gcgccgcagt aatgcagtta tgaaggcgat aaaaaagaat cgaatttttc
      901 atgtagcgct cacacggtgc tgttttcttc cttttttcta tccgatggac agtccacaaa
      961 gtcgtgacgt gtcgaccgat gcgctcacca ttgtggcgtt gggcgattct atcacatggg
     1021 gttatccgaa cggacactct tggacagaga tggtgcaaga agatacgggt atccgcatta
     1081 tcaaccgtgg cgtgaacggt gcaacattgc acaatatgct caaccgcatg ccgaccgatg
     1141 tattgccggt gaagccgtcc atttgcatca ttatgggagg tacaaacgac gccaaccaag
     1201 gccgtaccac caaagacatg atggcggata ttcaaaaaat ggcgaacgaa ctcatggcgc
     1261 atgacattat gccggtggtt gcgctgccga taccgatagt ttggcaaaaa ccggaagaga
     1321 agttacagga cttacgcgaa cgtattattt cctctgcatt tttaacgctt gactttgctt
     1381 tagatttcga tttaccgcgt gctgagttta aacaactttt gcccgatggg ttgcacccca
     1441 cagccgaagg taaaaaaatg atggcggagc gcctcaagaa agaaatgccg cgtcttttgg
     1501 cggcatacgc taagttcaga aaataaataa gccgttgacg ctctgtttcg tttatgtaga
     1561 ttgacaggtt agaccgacag gtcttttttt attggcttat tctttattgt ggtttatcca
     1621 tcgcgattta tccaacgaag gtatactagt aaaaacggga gg
//
LOCUS       DAOMPD010000565         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27520_length_1657_cov_2.874532, whole genome shotgun sequence.
ACCESSION   DAOMPD010000565 DAOMPD010000000
VERSION     DAOMPD010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27520_length_1657_cov_2.874532"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLY93_14220"
     CDS             <1..184
                     /locus_tag="PLY93_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70678.1"
                     /translation="IAAAMGKTSLALIPNHRKSRWQVYGEAQNAYFLYAEASPESLAV
                     DNIEPNEITEMIRGIV"
     gene            complement(199..708)
                     /locus_tag="PLY93_14225"
     CDS             complement(199..708)
                     /locus_tag="PLY93_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70679.1"
                     /translation="MAKRSVKITNIIEVHLPQQQWERIITLAVARKKTFSTMTRYCVL
                     RLVRKVNLRWTPVLIKAHGKAKREMQMGDSVHRHVMCLYGEDEMLIRLAVMRLSITMS
                     AFIRLALELFLPRLAMEKHGYSFITDEQLKWQAIRFVEKFTLTTQNIDSRPYTHRFNW
                     LAFALSTYW"
     gene            989..>1262
                     /locus_tag="PLY93_14230"
     CDS             989..>1262
                     /locus_tag="PLY93_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRP70680.1"
                     /translation="MELKNGPLTGVKVLDLSLLLPGPLCSQHLADMGAEVIKVENPRM
                     GDMTRYMGSKVTKDEYSETGFFLQLNRNKKSITVNIKRDGGRAVILR"
     assembly_gap    1263..1272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1273..>1657
                     /locus_tag="PLY93_14235"
     CDS             <1273..>1657
                     /locus_tag="PLY93_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRP70681.1"
                     /translation="DILLEGFRPDTLNDLGIGYEQLKEKFPRLIYCAISGYGATGVYK
                     EHAGHDANYIARSGLLALNGVKDGAPVVPGFQVADIGGGTLTALSSILAALYAREKTG
                     RGQFIDISMQDGAAQFLSLYMGEFVA"
BASE COUNT          444 a          392 c          354 g          457 t
ORIGIN      
        1 cattgccgcc gctatgggca aaacgagcct tgcgctcatc cccaaccacc gcaaaagccg
       61 ctggcaagtt tacggtgaag cgcagaacgc atacttcttg tacgcagagg cgtcacccga
      121 atcgctcgcg gtcgataata tcgagccgaa tgaaatcacc gaaatgatcc gagggattgt
      181 gtaagtttta gagatttatt accagtacgt gcttagcgca aaggcgagcc agttgaaacg
      241 gtgggtgtac gggcgagaat ctatgttttg agtagtaaga gtaaattttt caacaaagcg
      301 tatagcttgc catttgagtt gttcgtcagt tataaagcta tagccatgtt tttccatagc
      361 taatcgtggc aaaaacaact ctaaagcaag ccgaatgaac gctgacattg taatactcaa
      421 ccgcatcacc gccaatcgaa tgagcatctc atcttctccg tataaacaca tcacatgtcg
      481 atggacacta tcccccattt gcatttccct tttcgccttt ccatgcgcct ttataagcac
      541 tggcgtccac cgcaaattca ccttccgtac caaacgcagt acgcaatacc gtgtcatcgt
      601 tgaaaaagtc tttttacgcg ccacggccag cgtaataatc cgctcccatt gttgctgcgg
      661 caggtgtacc tctataatat tggtaatttt tactgaccgt tttgccaaaa agtctggttg
      721 atttctgcat ttcatcgtaa ttcctccatt ttagtgggat ttatcccttt cagtcctcaa
      781 tcctctactt attaaatacc aaaaaacact cgattttttt catttttaga aaaaaaaata
      841 tgagtttttg aaaaaaagag agatttcgtt aacgccacac agaaattcca tgctaatact
      901 gccttatagc tatggaattc cggtactata acattattta atacccttaa agagcttttc
      961 acacactctc aaaaataaac aatactgtat ggaactcaaa aacggcccat taacaggcgt
     1021 taaagtgctc gatttgtccc tcttgctgcc tggtccctta tgttcgcagc atttagccga
     1081 catgggcgct gaagtcatca aggtagaaaa cccacgcatg ggtgatatga cccgttatat
     1141 gggctcaaaa gtcacgaaag atgaatattc tgagacgggt ttttttttac agcttaatcg
     1201 caataaaaaa tcaatcaccg tcaacatcaa gcgcgacggt gggcgtgcgg tgattttgcg
     1261 ccnnnnnnnn nntgatattt tgctcgaagg cttccgtcct gatactctca atgacttggg
     1321 tattggttac gaacagctta aagaaaaatt tccacgatta atttattgtg cgatttcggg
     1381 ctatggagcc actggtgttt ataaagaaca cgcgggacac gatgccaact acatcgcgcg
     1441 ttcggggctt ttagcgctca atggcgttaa agacggtgcg ccggttgttc cggggttcca
     1501 agtcgccgat attggcggtg gtacgttgac cgcgctttcg tccattctag ccgcactcta
     1561 tgcgcgtgaa aaaacggggc gtggccagtt catcgacatt tcgatgcaag acggcgcggc
     1621 acaattcttg tcgctctaca tgggggagtt cgtcgcc
//
LOCUS       DAOMPD010000566         1655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27564_length_1655_cov_2.261875, whole genome shotgun sequence.
ACCESSION   DAOMPD010000566 DAOMPD010000000
VERSION     DAOMPD010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27564_length_1655_cov_2.261875"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLY93_14240"
     CDS             <1..822
                     /locus_tag="PLY93_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRP70682.1"
                     /translation="ETKVLAETLERLAATTPKELTRFKSIDENRRDILLPGAALLLTL
                     LKQAQINRFVVSPNGLRDGAVAEYIFNNLNKNVYVSSRTEFRETGLNAIIEKYKMDRA
                     HSHRVAALAVQLFDAFQELHLLPTEARDLLVAAAMLHDIGKFIDYSQHHKHTLYILSN
                     IKLHGYDDAEKQIIAHTARYHRKSSPKPAHLEYEALSNQKKAWILKLSAILRLADAFD
                     RASANSIDTVSAQKINSQKWNLSLGGSNILALEEWALENKKEVFEKIFSIKLVIV"
     gene            819..1655
                     /locus_tag="PLY93_14245"
                     /pseudo
     CDS             819..1655
                     /locus_tag="PLY93_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
BASE COUNT          430 a          430 c          396 g          399 t
ORIGIN      
        1 gaaactaaag ttttagcgga gacgctggag cgtttggcgg cgacaacgcc caaagaactc
       61 actcgtttta agagtatcga cgaaaaccgg cgcgatattc ttttgcctgg tgcggcgctg
      121 ctccttacgc tccttaagca agcccaaatt aaccgatttg tcgtttcacc caacggctta
      181 cgcgacggag cggttgccga atatattttt aataacctaa ataaaaatgt ttatgtcagc
      241 agtcgaactg agttccgaga aacggggtta aacgcgatta tcgaaaaata taaaatggat
      301 cgtgcacact cccaccgtgt cgccgctctc gctgttcaac tcttcgacgc attccaagag
      361 cttcacctac tccccaccga agcgcgcgat ttattagtcg ccgcagcgat gctacacgac
      421 atcgggaaat ttatcgacta ttcgcagcac cacaagcata ctctttacat cctcagcaat
      481 ataaagctgc acggttacga cgacgctgaa aaacaaatca tcgcccacac cgcacgctat
      541 caccgtaaat cttcacccaa accagcgcac cttgagtatg aagcgctctc caaccaaaaa
      601 aaggcatgga tactcaaact ctcggcgata ctgcgtttag ccgatgcatt cgaccgtgcc
      661 tcggcgaaca gcatcgacac agtgagcgct caaaaaataa attcacagaa gtggaattta
      721 agtttaggcg gctctaatat acttgctctc gaagagtggg cgctcgaaaa taaaaaagaa
      781 gtgtttgaaa aaattttttc gataaagttg gttattgtat gagtctggca ttggcgtttg
      841 aatcgagctg cgatgaaacc ggcgtggcat tggtggaaga tggcgtgcgt gtacttgccg
      901 caccgctttt tagccaaatc gccacgcacc gtccttacgg tggcatcgtg cccgagtacg
      961 ccagccgcgc acatttagaa aaattttcgt tactcatgcg tgagcttttg acgaaacacc
     1021 acttaaagcc gggagacatc gactacgttg ctgtcactgt acgcccgggg cttatcggtt
     1081 cgttgttggt gggctatcaa tcggcgcaag cggcagcgcg gtattttaaa gcgcccatca
     1141 ttggcgtcca ccacctcgaa gcgcatttgg ccgccattcg tttaagcggc cacgctctcc
     1201 tctacccttc gttgggactt ttactttcgg gtggcaattc ggcgctttac aaactcgacg
     1261 gtccaggacg catcgcggtt atcggcgaca cgcgcgacgg cgcagatctt gggctttggt
     1321 tatccaggtg gcccgctcat cgaaaaggca gcaaacgcgt acatggcatc cagaggcatt
     1381 tccttaggcg acgaattatc tatcgaacac gaaaatatgt ttcctaaaat tctaaaagac
     1441 ttaccgcgca caagcattgg ttttagtttc agtggaatta agactgcgct catgcgctac
     1501 catgcagaac atccagagaa gagcgttgaa gaacttgcat ttttttatca agagcgtatc
     1561 gtggagcata ttctacgcaa tctgacgcat gcgctcagca ccgtggggta tctgcccatc
     1621 gtcgcggcgg ggggcgggct tgcaaataaa cgctt
//
LOCUS       DAOMPD010000567         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27734_length_1647_cov_1.849874, whole genome shotgun sequence.
ACCESSION   DAOMPD010000567 DAOMPD010000000
VERSION     DAOMPD010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27734_length_1647_cov_1.849874"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLY93_14250"
     CDS             <1..359
                     /locus_tag="PLY93_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRP70683.1"
                     /translation="VFNFQLNKESVQTGVMLGNSIESFKALLAHTAKSGIPQALDFNL
                     TEWSRDLPIVIIEESIVLLETADPKLTEALLGQIRGKKIVKKEISDTALVIFKSKVQE
                     VMDAAEKLEMIVKLIR"
     gene            complement(387..>786)
                     /locus_tag="PLY93_14255"
     CDS             complement(387..>786)
                     /locus_tag="PLY93_14255"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRP70684.1"
                     /translation="HSDSVFRAIYESHPIDRLKLMARLLPKVEILPNNVVFLAVRRVD
                     IDGLSLGDSGTEGVVNYLLAHTGVKAAAVVKESEEGFLKASLRSLGDVDVATLAKKFG
                     GGGHKNAAGLKVKEPFAASMTKVRAELTKL"
     assembly_gap    787..796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<797..1471)
                     /locus_tag="PLY93_14260"
     CDS             complement(<797..1471)
                     /locus_tag="PLY93_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRP70685.1"
                     /translation="MKAEKETQNISANLAAALLAKYKPSSKVLPKIVAFIKKHDKFLL
                     TTHISADADGIGSQVGFYYLLKALKKDVKILNNEPVPQFLQGFMPTRLVGYIEDPSST
                     KKITGALSFAQAEKLARGRFVFILDSSEPKRSERVGELFMNLNLPWATIDHHVLANQK
                     EFCVDPTYAATAEMVWELYKYLKIKIPKEAALPLYTGLVADSGNFRYPKTSLRTHLAA
                     GELIGYG"
BASE COUNT          422 a          398 c          379 g          438 t
ORIGIN      
        1 aagtatttaa tttccagctt aacaaagagt cggtacaaac cggcgtcatg cttggtaatt
       61 ccatcgaaag ctttaaagcg ctgctcgcgc ataccgccaa aagcggcatc ccgcaggcgc
      121 tcgactttaa cctcaccgaa tggagccggg atctccccat cgtcattatc gaagagagta
      181 tagttctttt agagaccgcc gatcctaaac tcaccgaggc gctcttgggt caaatccgtg
      241 gcaaaaagat tgtcaaaaaa gaaatctccg ataccgcact cgtcattttc aaaagcaaag
      301 tacaagaagt gatggacgcc gctgaaaaac ttgagatgat tgtgaagttg attcgctaaa
      361 agtgcggcaa ctcgctggag taaattttac agttttgtca attcggccct tacttttgtc
      421 atggacgctg caaacggctc tttcacttta agaccagcgg catttttgtg accacccccg
      481 ccgaattttt tggcgagcgt ggcgacatcg acgtcaccca acgagcgtag cgacgctttc
      541 aaaaatccct cttcgctttc tttaacgacc gccgccgctt tgacgccagt gtgcgccaac
      601 agataattta cgaccccttc ggtaccgctg tcgcccaaag aaaggccatc gatgtcgacg
      661 cgcctcacgg ccaaaaaaac aacgttgttg ggtaaaattt cgactttggg taaaaggcgc
      721 gccatgagct ttaagcgatc gatgggatgg ctttcataga tggcgcgaaa tacagaatcg
      781 gaatggnnnn nnnnnnacca tagccgatga gctcacctgc agcgaggtgc gtgcgcagcg
      841 aagtttttgg gtatcggaaa tttcccgagt cggcgacaag gccggtgtaa agcgggagcg
      901 ccgcttcttt cggaattttt attttaagat acttatagag ttcccacacc atttctgcag
      961 tcgctgcgta cgtagggtcg acacaaaatt ctttttgatt tgccagtaca tggtgatcga
     1021 tcgtcgccca aggaagattg aggttcataa aaagctcacc cacccgctcg ctgcgcttgg
     1081 gctcggatga atcgagaata aagacaaacc gcccgcgtgc gagcttttcg gcttgtgcga
     1141 aggataacgc accggttatt ttttttgtac tcgatgggtc ttcgatatac cccacaagac
     1201 gtgtgggcat aaaaccttgt aaaaattgcg gcaccggttc attgtttaaa atttttacat
     1261 cttttttaag cgctttcaag agataataga aacccacttg actaccaatc ccgtcggcgt
     1321 cggcggatat atgcgtcgtc aataaaaact tgtcgtgctt tttgatgaat gctacaatct
     1381 taggaaggac tttcgacgac ggtttgtatt ttgcaagtaa tgccgccgcc aaatttgcac
     1441 tgatgttttg ggtttccttt tctgctttca tataaagtac attaatcctg cataaaattg
     1501 ctacgcaatt tcatgcagaa ttatttttga ggtgcctgcg cggcacgttt tttatctgcc
     1561 catgcgccca accacgaaag accatattct ttcgagtcga taaatgcggt ggtatagaaa
     1621 tcccgcacgt cgaccttgtc gcgcttt
//
LOCUS       DAOMPD010000568         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27826_length_1642_cov_2.585381, whole genome shotgun sequence.
ACCESSION   DAOMPD010000568 DAOMPD010000000
VERSION     DAOMPD010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27826_length_1642_cov_2.585381"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLY93_14265"
     CDS             complement(<1..550)
                     /locus_tag="PLY93_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRP70686.1"
                     /translation="MSTVIEKYDTIFIGSGIGTILAASLLARYRNEKILILERHFKLG
                     GFTHVFKRKNKFYWDVGIHYIGNLEEGSILSKLFDLLSGGTLKWQKMPDIFEKFVYPD
                     FTFAVSSDSERYKADLKQMFPQEAANIDAYFDDIKKVNTWFGRHIAIRGRAPFVDHLE
                     ADFLKKEGFNPRASTYDYLAHRF"
     gene            671..1495
                     /gene="speD"
                     /locus_tag="PLY93_14270"
     CDS             671..1495
                     /gene="speD"
                     /locus_tag="PLY93_14270"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRP70687.1"
                     /translation="MKIKPLKKKLKLYGFNNLTKTLSFNMYDICYAASVKNQQAYIEY
                     IDEEYNAQRLTEILTNVAKIIGANILNIASQDYDPQGASVTMLVSENHVEVEKRNLLD
                     PSREMPGPESEAVVAHLDKSHITVHTYPERHPDQGISTFRADIDVSTCGQISPLKALN
                     YLLHSFSPDIVMSDYRVRGFTRDVDGKKYFIDHKINSIQNFISEDLRDMYQMVDINEY
                     QENIFHTKMILKEFNLDNYLFGSAKKDLPPGDKKKIKQRLKREMNEIFYGRNISKM"
BASE COUNT          494 a          400 c          338 g          410 t
ORIGIN      
        1 gaaaacggtg cgctaaatag tcatacgtcg atgcacgggg attaaatcct tcttttttaa
       61 gaaaatccgc ttcgaggtga tcgacaaacg gcgcgcgccc acggatggca atgtgacgac
      121 caaaccatgt atttactttt ttgatgtcgt cgaaatacgc gtcgatgttc gccgcttctt
      181 gggggaacat ttgcttgagg tcggccttgt agcgctcact gtcgctcgag acggcaaacg
      241 tgaaatcggg gtacacaaat ttttcaaaaa tgtcaggcat cttttgccac ttgagcgtac
      301 cgccggagag taaatcaaaa agcttactga ggattgaacc ttcttctaaa ttaccgatgt
      361 aatggatgcc gacatcccaa taaaatttat ttttacgctt aaagacgtgc gtgaaaccgc
      421 caagtttgaa gtgcctctct aaaattaata ttttctcatt acggtatcgc gctaaaagcg
      481 aggcagcaag aatggtgcca atccccgaac cgatgaaaat agtatcgtat ttttcaataa
      541 ctgttgacat actttaaggt tacgcacaag atagacaaag agccattgtg tcaacgtgaa
      601 agttcttcac ttaacgattg acccgctgtt ttttgtactc attgcgtctt aactcacgca
      661 agataataca atgaaaatta aaccactcaa gaaaaaactc aagctctatg gctttaacaa
      721 cctcaccaaa acgctgagtt tcaatatgta cgacatttgt tacgcggcga gcgtaaaaaa
      781 tcaacaggcg tacatcgaat acatcgacga agaatacaac gcgcaacgcc tcaccgaaat
      841 tttaaccaac gttgccaaaa ttattggtgc gaatattttg aacatcgcct cacaagatta
      901 cgacccgcaa ggcgcatcgg tgacgatgct ggtctcagaa aatcacgtcg aagtggagaa
      961 acggaacctg ctcgacccct cgcgcgaaat gcccgggcct gaatccgagg cggtggtcgc
     1021 gcacctcgac aagagccata tcaccgtaca tacctacccc gaaagacacc ccgaccaagg
     1081 tatctcaact ttccgcgccg acatcgacgt ctctacgtgc ggccaaattt caccgctcaa
     1141 agcgctcaac tatcttttac actcgtttag ccccgacatt gtgatgagcg actaccgtgt
     1201 gcgcggattc acacgcgatg tggacggcaa aaaatatttt atcgaccaca aaattaactc
     1261 gatccaaaat tttatttcgg aagacttgcg cgacatgtat cagatggtcg atatcaacga
     1321 gtatcaagaa aatatctttc acaccaaaat gattctcaaa gagttcaatt tagacaacta
     1381 tctttttggt tctgcgaaaa aagaccttcc gccgggtgac aaaaagaaaa ttaaacaacg
     1441 cttgaagcgc gagatgaacg aaattttcta cgggcgcaac atatctaaaa tgtaaagtgc
     1501 tctctttgcg cgaaccgctc tttttctcct cccgctataa cgacattttt ttcgacggag
     1561 aaagtcccac gttggaaaaa aattttgttt tcctgaaagc aaaccgcgtg tacgagcggc
     1621 tcggtgcgct ctctaccaac gc
//
LOCUS       DAOMPD010000569         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_27888_length_1639_cov_2.203283, whole genome shotgun sequence.
ACCESSION   DAOMPD010000569 DAOMPD010000000
VERSION     DAOMPD010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27888_length_1639_cov_2.203283"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLY93_14275"
     CDS             complement(<1..916)
                     /locus_tag="PLY93_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70688.1"
                     /translation="MSAIGLRRVNPSRWLVAAALAVIVQLVCTKTPPSEFLSFDDSVE
                     YPPPDAQGNPEMFSSLPLAPEAETQLQTHTDTHYRTLPIPPTSHEGDEDIDAAIQHLS
                     VVVHEQPEIEEINSALLEAATGCTMPRVSMANPVLTDGAHWKVFAKCVLQTTPQGQLL
                     YGFVPELQQYGSAAGLMWSMNQLALLKHKMWQRPNAEKLNYLERLVVATRFRLPTLAE
                     KNAAAAAIEENGIVNASFIYLMNWYYSLYYQKHFDYAKRPSPRYSSYIVSGRFVNAQN
                     QNLQGVKYSVPIEFLAGCVLKGITGANGA"
     gene            complement(971..>1639)
                     /locus_tag="PLY93_14280"
     CDS             complement(971..>1639)
                     /locus_tag="PLY93_14280"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRP70689.1"
                     /translation="DSHAMQYGTALEKVAQMHNWRLLVLTKPACPFGEFPVYYEGWER
                     AECTFWKKEVLKIITAQKPDLLIIGTARAGLFGQIAGSEKQIAGYKSYWQKYLDAGIR
                     VAVIKDNPLMRGGKHRYMTPAGCALTNAKNVAVCESPRQDALESLKDYLVVVQEEMKD
                     RVSLIDLSDIFCKKETCAAVIGNILVYRDRHHITDVFGKTLAPYFDKIIKDLLKKPAP
                     QPKS"
BASE COUNT          383 a          405 c          407 g          444 t
ORIGIN      
        1 ttgcaccgtt tgcaccggtt atacctttca aaacacaacc cgccaaaaat tcgataggta
       61 cgctatactt gacgccttgc agattttgat tctgtgcgtt gacgaagcgc cccgaaacga
      121 tgtacgaact ataacgaggg ctcggcctct tggcgtagtc aaaatgcttt tgataatata
      181 gtgagtaata ccagttcatc aaataaataa acgaagcgtt tacaatgccg ttctcttcga
      241 ttgccgccgc tgccgcattc ttttctgcga gtgtcggcaa acgaaagcgc gtcgcaacaa
      301 ccaggcgctc cagataattg agtttctctg cgtttggtct ttgccacatt ttgtgtttca
      361 aaagcgcgag ttggttcatg ctccacataa ggcctgcagc gctaccgtat tgttgtagtt
      421 cgggaacaaa accgtaaaga agctgccctt gcggcgtcgt ctgtaacacg cacttggcga
      481 aaactttcca atgtgcaccg tctgttaaaa cagggtttgc catggataca cgcggcattg
      541 tgcagccggt tgccgcttca agtaatgcag aattgatttc ttcgatttcg ggttgttcat
      601 gcaccaccac cgacaagtgt tgaattgccg catcgatgtc ttcgtcgcct tcgtgagagg
      661 tgggcggtat aggcagcgtt ctatagtgcg tatcggtatg tgtttgtagt tgcgtctctg
      721 cctcgggtgc aagcggcagc gaagaaaaca tttcaggatt accctgtgca tccggaggtg
      781 gatattcgac gctatcgtcg aaagacaaaa actcactcgg cggcgttttt gtgcaaacaa
      841 gctgcacaat aactgccagc gccgcagcaa caagccagcg actcggattt actcgacgta
      901 acccgatagc agacatatcg atatattggt ctgcctctgg aatggatgcc agaaatataa
      961 aaagtcgctt ttaactctta ggctgtggcg ctggtttttt caagagatct tttataattt
     1021 tatcaaagta cggagcgagc gttttgccga atacgtcggt gatgtggtgg cggtcgcggt
     1081 acaccaaaat gttgccgatg accgctgcgc aggtttcttt cttacagaaa atatcgctta
     1141 aatcgatgag cgagactcgg tctttcattt cttcttgcac caccaccaaa taatctttga
     1201 gcgattcgag agcgtcttga cgcgggcttt cgcacaccgc gacgttttta gcgttggtga
     1261 gcgcacaacc tgccggcgtc atgtagcgat gcttaccacc gcgcatcaag gggttgtctt
     1321 tgatgacggc gacgcggata cccgcgtcga ggtatttttg ccagtacgat ttataccctg
     1381 caatttgttt ttcactccct gcaatttggc cgaaaagacc cgcgcgggcg gtgccgatga
     1441 tgaggagatc aggcttttgc gcggtgataa ttttcaacac ttctttcttc cagaacgtac
     1501 actccgctct ttcccaacct tcgtaataca ccggaaattc accaaaggga cacgcgggct
     1561 tggtgagcac aaggagtcgc cagttgtgca tttgcgccac tttttcaagc gccgtgccat
     1621 attgcattgc gtgcgagtc
//
LOCUS       DAOMPD010000570         1629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28079_length_1629_cov_1.901525, whole genome shotgun sequence.
ACCESSION   DAOMPD010000570 DAOMPD010000000
VERSION     DAOMPD010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28079_length_1629_cov_1.901525"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLY93_14285"
     CDS             <1..513
                     /locus_tag="PLY93_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70690.1"
                     /translation="ALAAAPNRSNCTAFETDAVSCRTLPGASAYTLVAGKPMGSYQID
                     QAVFEMRSVYKGFYFKQETHAKRIIDRAGILPEAQLWGALLQAGVFPHTVLSFIPPQL
                     ETAARFSFIDTNSKNVGVYQEEYSGVLNWFERGHLNKISLEVTHHRLKNLTGGYATEE
                     RYRAQWDISF"
     gene            555..1076
                     /locus_tag="PLY93_14290"
     CDS             555..1076
                     /locus_tag="PLY93_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70691.1"
                     /translation="MKARNVLVVLGLIAAVAATRFFSHPMNFSPVMSVALFGAAVFFN
                     RYLGIAVAILAMAVSDIFLGLHSTLPFVYASMALAGVLGFFLREKRSPLKIAAMAISG
                     SVLFFIITNFGVFLMTGMYPYTLEGLLTCYTMALPFFKNSLAGDLVFSGSLFTLYYFF
                     VKRRFTASTANAT"
     gene            1077..>1629
                     /locus_tag="PLY93_14295"
     CDS             1077..>1629
                     /locus_tag="PLY93_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP70692.1"
                     /translation="MSDDPLPLLSFTGVTLARAGKELLGEVTLSVFEGDRVHLEAENG
                     AGKSSLLLLMAGRLHPHNNTGTRAYAWDAPPAVDFRQARKNIALISRDEDERLKKIHH
                     KSTVREFLLGHLDGQDFLYREVEAFDVPKVRAALSIFSLEYLENRLVRTLSLGEMRLC
                     TLARMALHERRLYLFDEVLNSLAP"
BASE COUNT          367 a          388 c          407 g          467 t
ORIGIN      
        1 gcactcgctg cagcgcccaa ccgcagcaac tgcactgcgt ttgaaacaga cgcggtaagc
       61 tgccgtactt tacccggggc cagtgcatat acgcttgtag cgggaaagcc tatgggaagc
      121 tatcaaatag accaagcggt tttcgagatg cgctcggtgt ataaaggttt ttatttcaag
      181 caagagacgc atgcaaaacg tatcatagac agagcgggta ttcttccaga agcgcaactg
      241 tggggtgcgc ttctgcaagc aggtgtcttt ccgcataccg tgctgagttt tattcctcca
      301 caattggaaa ccgccgcacg attttcgttt atcgatacca actcaaagaa tgtgggtgtg
      361 tatcaagaag agtactcggg ggtattgaat tggtttgagc gaggccattt gaataaaatt
      421 tcacttgagg tgacgcacca ccgcttaaaa aaccttacag gaggatacgc gaccgaagaa
      481 cgttaccgcg cgcagtggga tatttcgttt tagtattcac cttgacggcc tctttctttc
      541 cttaaagctg aagcatgaaa gcacgaaatg ttctcgttgt tcttggtctt atcgctgccg
      601 tcgcagcgac acgctttttt tcgcatccga tgaatttttc accggtgatg agcgttgccc
      661 tttttggcgc agcggtgttc tttaaccgtt acctcggtat cgctgttgca attttagcga
      721 tggcagtgtc cgatattttt ttgggtctcc attcaaccct cccttttgtc tatgcttcga
      781 tggcattggc aggtgttttg ggctttttct tgcgtgaaaa acgctcaccg cttaaaattg
      841 cagcgatggc aataagcggt agcgttctct tctttattat taccaacttc ggtgtctttt
      901 taatgaccgg tatgtatccc tatactcttg aagggcttct cacctgttat actatggcgc
      961 tcccgttttt caaaaattct ctggcggggg atttagtttt tagtggttca ctttttacac
     1021 tgtattattt tttcgtaaag cggagattta cagcgtcgac ggcgaatgca acgtagttga
     1081 gcgacgatcc cctccctctc ctttcgttca ccggtgtcac cctcgcacgt gccggaaaag
     1141 agcttctggg tgaggtaacg ctttccgttt ttgaaggcga tcgcgtccat ttagaagcag
     1201 aaaacggcgc tggcaaaagc tcgttgctgt tgctcatggc ggggcgtctc catccgcaca
     1261 acaacaccgg cacacgcgcg tatgcgtggg atgctccacc ggcagtcgat ttccggcaag
     1321 cgcgtaaaaa cattgcactt atcagccgcg acgaagacga gcggcttaaa aaaatccatc
     1381 acaagagcac ggtgcgtgaa tttttgctag gccatctgga tggccaggat ttcctctatc
     1441 gggaggtaga agcgttcgac gtcccaaaag ttagagcggc actgtcaatt ttttctctgg
     1501 agtatttaga gaatcgtttg gtgcgtacgc tttctttagg tgagatgcgg ctttgcacac
     1561 tcgcacggat ggcgctccac gagaggcggc tatacctttt tgatgaagta ttaaattcat
     1621 tagctcccg
//
LOCUS       DAOMPD010000571         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28118_length_1627_cov_2.224555, whole genome shotgun sequence.
ACCESSION   DAOMPD010000571 DAOMPD010000000
VERSION     DAOMPD010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28118_length_1627_cov_2.224555"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..529
                     /locus_tag="PLY93_14300"
     CDS             107..529
                     /locus_tag="PLY93_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70693.1"
                     /translation="MRKRFTLLLFAITLALHAEIHPKYYREMQQNAPEDLRLKIIDVS
                     RDWRFWSDTRHVKVKAVVVEVKKSQVGLTPNTHVFFEYDVFTPSGGWVGPRPMPLLEE
                     NSTTDFFGERIDVDKKRGVILLPTARGYSFDRIHDGDN"
     gene            513..920
                     /locus_tag="PLY93_14305"
     CDS             513..920
                     /locus_tag="PLY93_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HRP70694.1"
                     /translation="MTATTNSNTNPIVFFDGVCNLCNASVDFLIQRDKRHLLRFAPLS
                     GKTAAALLAQKTIPDSIVLYIDGQIFTRSTAALKLVRFLPWPWQILRVGWLLPSFLRN
                     ALYDFIARHRYRWFGKKETCRLPTPAEKALFLE"
     gene            1053..>1627
                     /locus_tag="PLY93_14310"
     CDS             1053..>1627
                     /locus_tag="PLY93_14310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRP70695.1"
                     /translation="MANIDQKIKVRKINFGHETEENRHWFRDNQIMTHFMNSLHSVFP
                     DGERLFIRSVNYFAKEIKNTELKERVKAFIGQEVQHGSQHERFLQALDKMGLEGTKFE
                     EWYAKNAYVSKEDKLSAERILIGTVRAILGDKAGDRMSLSATAALEHYTATLGEVILK
                     NEEFYTKGMPENLKQLFLWHAAEEVEHKSVA"
BASE COUNT          469 a          380 c          363 g          415 t
ORIGIN      
        1 aatacccccc aattgcggcg tagcatttgt caggtatgtc cgtgggttag acacaaaaaa
       61 caagaaaagc gcgtgacgcc aataacggca tcgcgattga tgcgctatgc gtaagcggtt
      121 tactcttctt ctttttgcta taaccctcgc tctccatgca gaaatacatc ctaaatacta
      181 tcgagaaatg cagcaaaatg cccccgagga tttgcgtctt aaaattatcg atgtgtcgcg
      241 cgattggcgt ttttggagcg atacccgtca cgtcaaagtc aaagcggttg tagttgaggt
      301 aaaaaaaagc caagtgggat taaccccgaa cacacatgtt ttttttgaat acgatgtttt
      361 caccccttca ggcggatggg ttgggccgcg ccccatgccg cttttagaag aaaactcgac
      421 aaccgatttt tttggcgagc gtatcgatgt cgataaaaag cgcggggtga ttttactccc
      481 tactgcacgc gggtactcgt ttgatcgcat ccatgacggc gacaactaat tctaacacaa
      541 atcccatcgt tttttttgac ggggtatgca atctctgtaa cgccagcgtt gattttttaa
      601 tccaacgcga caaacgccac cttctacggt ttgcaccgct ctcggggaaa acggcggcgg
      661 cgttgttagc acaaaagaca atccccgact cgattgtgct ttatatcgat ggccaaattt
      721 ttacccgtag cacggccgcg ctcaaacttg tgcgatttct tccatggcca tggcaaattc
      781 tgcgtgtggg gtggctccta ccttcttttc tacgtaacgc gctttacgat tttattgcac
      841 gccaccgcta tcgttggttt ggtaaaaaag agacgtgccg gttacccact cctgctgaaa
      901 aagcgctgtt tttagagtaa caataataag tttgagaact ccccaacaca agaaaccaaa
      961 aaaaattgac actaagttca ccttttgaga tatatataaa caatggttta cgatgcgctg
     1021 caaaggcgca gtgcgcaaac tttggaggca aaatggcaaa tatagatcaa aaaatcaaag
     1081 tgcgtaaaat taattttggg cacgaaacag aggagaatcg tcattggttt cgcgacaacc
     1141 aaatcatgac acacttcatg aattcgttgc attcggtatt ccccgacggc gaacgtcttt
     1201 ttatccgtag cgtcaattat ttcgcgaaag aaataaaaaa cactgaactc aaagagcgtg
     1261 taaaagcgtt catcggccaa gaagtacaac acggttcgca acacgaacgc tttttgcaag
     1321 cactcgataa aatggggctt gaaggcacga aatttgaaga atggtatgca aaaaacgcat
     1381 acgtctctaa agaagataaa ctttctgccg agcgtatcct catcggtacc gttcgcgcta
     1441 ttttgggcga caaagcaggc gatcgcatgt ctctttccgc cacagcagcg ttggagcact
     1501 acaccgcaac attgggcgaa gtcatcttga aaaacgaaga gttttatacc aaaggcatgc
     1561 ctgaaaactt gaagcaactt ttcctttggc atgcagccga ggaggtcgaa cataaatcgg
     1621 tagcatt
//
LOCUS       DAOMPD010000572         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28238_length_1621_cov_2.485313, whole genome shotgun sequence.
ACCESSION   DAOMPD010000572 DAOMPD010000000
VERSION     DAOMPD010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28238_length_1621_cov_2.485313"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            615..>1621
                     /locus_tag="PLY93_14315"
     CDS             615..>1621
                     /locus_tag="PLY93_14315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRP70696.1"
                     /translation="MRINLGPSHPSTHGILENHLVLDGETIESADVVIGYVHRCFEKL
                     GEHYGYNQFLVCTDRMNYVSTPMNNIGWILAVESLMGITAPPKALVVRMIIAELSRII
                     DHIICLGILGVDLGAFASFLYMFHQREDVYQILEKLTGARINNTFCRIGGLERDIYPE
                     FQADVKVFLKNFPKVLKETDALLTKSRIFIDRTKGVGKISAERALEYGFTGPNLRAAG
                     VDFDVRVSNPYMYYDQVDFDIPVGEDGGVYDRFLVRLEEMRQSLRIIEQLNNNIPEGA
                     YHADIPHAYLPSKQKVYSSMEDLIYHFKIIQHGITVPTGEHYFSTEAANGELGFTIVS
                     RG"
BASE COUNT          448 a          402 c          358 g          413 t
ORIGIN      
        1 tctcatcgtt tcaaatggtt tacgtttgca ctaacatcct tttggtaaca taaaatttca
       61 tacgttctca aacaccaaag aacttagaag ctgacggata ctcgtctacc gcagttaaga
      121 aatttgctgt aacaatatct tgaattatac ttcaactata tctcgcactc tcatgcgtct
      181 gacgatacgt gaattagcgc tacggcaggg tgttgaatga tggggattta tccattgcgg
      241 ttgactaccg caggatttga accgaacgag ccgcagtagt cgcgaaaaaa atacggaagt
      301 aaaacgcggt gaaaattcca tatacggaac gttactttat aaaacgagaa aatccggctt
      361 attaactcag cacacggcaa ctcttcacac ccttcagctt accggtagga ttgatttgcc
      421 acatcgcatt aaagcataga taattatgaa atcagtttac accgtagccg aatctcccgt
      481 acgtattcta tatcatgaag ggctctgtcg acgcggataa tcgcaatttt ctccatgtac
      541 cacctcccac agaaacacag tggcggcctg ttgcacaagc gggagtaaaa attgctgacc
      601 tacccgacgg tcgtatgcgc atcaacttgg ggccatcgca cccgtcaaca cacggcattc
      661 tggaaaatca tttggtgttg gatggcgaaa cgattgaatc agcagatgtc gtcattggtt
      721 atgtacaccg ctgctttgaa aaattaggcg agcactacgg ttacaaccaa ttcttagtat
      781 gtaccgaccg catgaactat gtctcgacac ccatgaataa catcggttgg attctcgctg
      841 tcgagagcct catgggaatc actgcgccac ccaaagcgct tgttgtgcgc atgattatcg
      901 ctgagctttc gcgcatcatc gaccatatta tttgcctcgg cattttaggt gtcgacttgg
      961 gagcattcgc atcgtttctt tatatgttcc accaacgcga agacgtgtac caaattttag
     1021 aaaagctcac cggcgcgcgc atcaacaata ctttctgtcg catcggtgga cttgagcggg
     1081 acatctaccc cgagtttcag gcagacgtca aagtgttttt aaaaaatttt ccgaaagtac
     1141 taaaagagac cgatgcgcta cttaccaagt cgcggatttt tatcgaccgc acgaaaggcg
     1201 taggtaaaat ttcagccgag cgggcgctcg aatatggttt caccggcccc aacctacgcg
     1261 cagcgggcgt cgacttcgac gtacgcgttt caaacccgta catgtactac gaccaagtcg
     1321 attttgatat tccggtgggg gaagacggcg gcgtgtacga ccgctttttg gtgcgtttgg
     1381 aagagatgcg tcaatcgcta cgcattattg agcaactcaa caacaacatt cccgaaggcg
     1441 cgtaccacgc cgatataccg cacgcgtatt tgccttcaaa gcagaaagtg tatagctcaa
     1501 tggaagacct catctatcac ttcaaaatta tccaacacgg gataactgtc cccaccggtg
     1561 agcactattt ttcaaccgaa gcagcaaacg gcgaattagg attcaccatt gtttcacgcg
     1621 g
//
LOCUS       DAOMPD010000573         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28369_length_1615_cov_2.349359, whole genome shotgun sequence.
ACCESSION   DAOMPD010000573 DAOMPD010000000
VERSION     DAOMPD010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28369_length_1615_cov_2.349359"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..593)
                     /locus_tag="PLY93_14320"
     CDS             complement(141..593)
                     /locus_tag="PLY93_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70697.1"
                     /translation="MKVKRLIYFIPLAFVSCMQNEKITLEYINAPVDLIVTSMDASTY
                     ELSFYSDNREGNFSGYGVFVGVSAAAVNTDTDSLPIDTSAATAFCATSSQIIYASRVK
                     IRVGATSGSDTLCNLTALSLSSGQFVGLRARVEREEKAWSHAAIARAP"
     gene            838..>1615
                     /locus_tag="PLY93_14325"
     CDS             838..>1615
                     /locus_tag="PLY93_14325"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRP70698.1"
                     /translation="MRILILGAGGREHALYHKIKKSPHAERVFVWPGNGLIDTHDRVL
                     QSAIEADFEPLKDFILTQKIDFTVVGNEAPLVHGVRDYLKKEIPQHRVFGPDKLGATL
                     EGSKAFADAFMVEAKIPHGKSHIAKNLADARAALQKQTLPLVIKADGLAAGKGVSIHH
                     DFDSAEKKIFEIFENKIFGASGSQVLLQQFLYGTEASLFALCNGNEAFLLPVARDYKR
                     ALTGDNGENTGGMGAYVPGTHLTDGQKLFTLERIINPVLKK"
BASE COUNT          443 a          398 c          357 g          417 t
ORIGIN      
        1 tggttagagc gctaccttga catggtagag gtcactggtt cgaatccagt atttcgcaca
       61 tttttttctc agcgagttgg cgcacaggct cctttgtcgt ataattatct aaaagtctac
      121 cgactcttcg aggctctccc ttaaggcgct cgtgcaatcg ccgcgtgtga ccacgctttt
      181 tcttcacgct caacccgcgc acgcaatccg acaaattggc ctgagctcag tgaaagtgca
      241 gttaagttac aaagcgtatc cgaaccactc gttgcgccga cgcgaatctt aacgcggctt
      301 gcatagataa tctgtgaact cgtcgcacaa aatgcagtcg ctgcggacgt gtctattggc
      361 aaagaatcgg tgtcagtatt caccgctgct gcgctcaccc ccacaaaaac accatagccg
      421 gaaaaattcc cttcgcggtt gtcggaataa aaactcaatt catacgtgct cgcatccata
      481 gacgtgacga ttaaatccac cggtgcgttg atatactcca aggtaatttt ttcattttgc
      541 atacaagata caaacgctaa aggaataaaa taaataaggc gcttcacttt catgtaaaac
      601 tctactttta tgctcgtgcg tcaatcgtaa acgagcgctg gtgggagcct atgagcctgt
      661 gcgtcaactc gcaaagcata gaaaaggaga aagatttttg cacattttga tcctccaacg
      721 ctttgagaat atagttcccc tcaacgacaa atttagtatt ttttgacgcc ttttctcaga
      781 cttgcacgag atcataaaat cgatttacgc gcactcttta aggcgcactt cagtggcgtg
      841 cgtattttaa tcttaggcgc gggggggcgc gagcatgcgc tttaccataa aatcaaaaaa
      901 tcccctcacg ccgaacgtgt ttttgtttgg ccggggaatg gccttatcga tacccatgac
      961 agagtcttac agagcgctat agaggccgat tttgagccac tcaaagattt tattttaact
     1021 caaaaaattg attttaccgt agtgggtaac gaagcgccgc ttgtccacgg tgtacgcgat
     1081 tacttaaaaa aggaaatccc acagcatcgt gtctttggac ccgataagtt gggagcaaca
     1141 ctcgagggct ctaaagcatt cgccgacgcg tttatggtcg aggcaaaaat tccgcatggc
     1201 aaaagtcaca tcgcgaaaaa tttagcagac gcgcgcgccg cactccaaaa gcaaactttg
     1261 ccgttggtta tcaaagctga tggcctcgcc gcagggaaag gcgtcagtat ccatcacgat
     1321 tttgactcgg cagaaaaaaa gatttttgaa atttttgaaa ataaaatttt cggtgcgtcg
     1381 ggttcgcaag tacttctgca acaattttta tacggcaccg aagcatcgct tttcgctctc
     1441 tgcaatggca acgaagcgtt tcttctgccc gttgcgcgtg actataaacg cgctctcacc
     1501 ggagacaacg gcgagaacac cggcggcatg ggagcttatg taccaggcac gcacttaacc
     1561 gatggccaaa aacttttcac ccttgaaaga attattaacc ctgtactcaa aaaat
//
LOCUS       DAOMPD010000574         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28428_length_1612_cov_2.444444, whole genome shotgun sequence.
ACCESSION   DAOMPD010000574 DAOMPD010000000
VERSION     DAOMPD010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28428_length_1612_cov_2.444444"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLY93_14330"
     CDS             complement(<1..706)
                     /locus_tag="PLY93_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70699.1"
                     /translation="MKLYINSLYLLFLFMGFSFLQAETIITTQGIVKGKIIDAQTEFV
                     AVQEDASMPRVIPRVEILRIFDDTQTLVWQNPTIVHEDKTTVKPQEETPWRRNFDIGV
                     KGGLHLLWPNGTLSAMPLGISPGYQLAFDGAAVAAWYFADDAALTAGLGYARRPVALQ
                     GILEGGQVHKGVWSIDDGILQLGYRMHSGIFFLEGGLDLAFPLANAALQLTPSATSVK
                     TPYTTPFSLSFYIAVGA"
     gene            complement(703..1503)
                     /locus_tag="PLY93_14335"
     CDS             complement(703..1503)
                     /locus_tag="PLY93_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRP70700.1"
                     /translation="MRRWRKKLHEIIYEADTTAGKIFDITLIIIILASVLVIMLDSVQ
                     TLQASFHSAFSVLEWFFTVVFTIEYVLRLWSVERPLKYALSFYGVVDILSIIPTWAGL
                     FFPQAHYLLSVRVLRVLRIFRVLKLANYLTQVEVLKQALVESRRKITVFLITVFTIAI
                     IVGSLIYIIEGGENGFSSIPRGIYWAIVTLTTVGYGDIAPKTNFGQALATIVMVMGYA
                     IIAVPTGIVTAELTQLRIKKVSTQACSSCASEGHDSNAKFCKFCGAKL"
BASE COUNT          463 a          371 c          372 g          406 t
ORIGIN      
        1 gcgcccctac tgcgatgtag aacgaaagag aaaaaggagt cgtgtagggt gtctttacag
       61 aagtcgccga tggcgttaat tgaagcgccg cattcgcaag aggaaaggca agatctaacc
      121 ctccctcaag aaaaaatata cccgaatgca tgcggtatcc caactgcaaa attccatcgt
      181 cgatactcca aacacctttg tgcacttgcc caccttcgag aataccttgc agggcaacgg
      241 gacggcgggc ataccccaag cccgcagtga gtgcagcgtc gtcggcaaag taccatgcgg
      301 caaccgcagc gccgtcgaat gcgagttggt atccggggga aatccccagc ggcattgcac
      361 tgagtgtacc gttgggccac aagaggtgca atccgccttt cacgccgata tcaaaattgc
      421 gccgccatgg tgtttcctct tgcggtttta ccgttgtttt atcttcatgg acgattgttg
      481 ggttttgcca cacgagtgtt tgtgtatcgt cgaagatacg caaaatttca acacggggta
      541 ttactcgcgg cattgacgcg tcttcttgaa cagcgacaaa ctccgtttgt gcatcgataa
      601 ttttaccttt aacaatgcct tgggtagtga tgatagtttc cgcttgcagg aaagaaaaac
      661 ccataaaaag gaataataga tacaatgagt tgatataaag tttcatagtt ttgctccaca
      721 aaacttacag aactttgcat tggaatcatg tccctcggat gcacacgatg aacaagcttg
      781 ggttgaaact ttttttatcc gcagttgagt taactctgca gtgacaatcc ccgttggcac
      841 ggcaataatt gcatacccca taaccattac aattgtggct aacgcttgtc caaaatttgt
      901 tttaggagca atgtcgccgt acccaacggt cgtgagcgtg acaatcgccc aatagatacc
      961 gcgtggaata ctactgaaac cgttttcacc gccttcgata atatagataa gagatcccac
     1021 aataatggca atcgtaaaaa cagtaatcag aaaaacggtg attttgcgac ggctttcaac
     1081 gagcgcttgt ttcaaaactt cgacctgcgt taaataatta gcaagtttta gtacgcgaaa
     1141 aattcgcagt acgcgcagta cgcgtaccga gagaagataa tgcgcttggg gaaaaaagag
     1201 gccagcccat gttggtataa tcgatagaat gtcgacgaca ccataaaagc ttagcgcgta
     1261 cttgagaggg cgttcaacag accataagcg aagtacgtat tcgattgtaa agactaccgt
     1321 aaaaaaccac tctaagacag aaaaggccga atggaacgac gcttgtagcg tctgtacgct
     1381 atcgagcatg ataaccagta cgctggctaa aattataata ataagggtga tatcgaaaat
     1441 tttacctgcg gtggtatccg cttcataaat gatttcgtgt aatttttttc gccaccggcg
     1501 cataaagaat tgtcagaact tattttaatt cgtcggcggt tttgcgtgtg gcagcgatac
     1561 cattttcgta ccagtccgca cgactgtccc aatgcacgtt accgtcgact tt
//
LOCUS       DAOMPD010000575         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28481_length_1610_cov_1.978135, whole genome shotgun sequence.
ACCESSION   DAOMPD010000575 DAOMPD010000000
VERSION     DAOMPD010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28481_length_1610_cov_1.978135"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..642
                     /locus_tag="PLY93_14340"
     CDS             145..642
                     /locus_tag="PLY93_14340"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HRP70701.1"
                     /translation="MSQSFTLTQKFAASREHVLAARERRFEDLSKHDGVKKQEILSET
                     HEGAVKITKRAFSLADKVPDMVKSMMPAGFLNLTETARFDTETKTNRFDVTYEQNPDR
                     MQIKGETKYIDEGENSSRREYNITVKVNIPLVGSLLEIQIASSFKKGIEKDYQNIQDI
                     LAAEK"
     gene            639..>839
                     /locus_tag="PLY93_14345"
     CDS             639..>839
                     /locus_tag="PLY93_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70702.1"
                     /translation="MKQNIIDHPPLGIITNPYAGKNAAISHRSKELQRIIGKHGIVKE
                     SRNLDQLKSILSEFAAHRVPYIV"
     assembly_gap    840..849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <850..>1610
                     /locus_tag="PLY93_14350"
     CDS             <850..>1610
                     /locus_tag="PLY93_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70703.1"
                     /translation="CDITYDPGQYAVCDGGDGTIHWIVNAYLEVLQEKNVAALEAAFP
                     ILIPTNGGTIDFLARKVGAKGGTYKILRKLRKTLDKGPLPQPVTLRTFMMEGTYTKEH
                     LGNEYQKLAFAGALAGFTQKFFNKYYESGKPSLQTIVEVITKTFTSTAFKGSWFENYV
                     PKDVINYSNEIFDPFAAEVKVDGKPIPYSRLNTLNVGSIAIEIKNLIKLFANADRDQK
                     LHMHVGYLEPFEVFSNLGNLFAGTDYSGKELVQTTA"
BASE COUNT          482 a          400 c          341 g          377 t
ORIGIN      
        1 aaagctatcc ttcaggcggg caggtcaccg tttcttcacc ccactgagac catagcacca
       61 cccagtgatt gaatttgtac gttacgccaa ggttaatttt aggattgccg caccctctac
      121 caacgctttc ctggcctatt cgatatgtca cagtcgttta cactcactca aaaatttgca
      181 gcctcgcgcg aacatgtctt agcggcacgt gaacgccgct ttgaagatct ttccaagcac
      241 gatggcgtca agaaacaaga aatcttgagc gaaacgcacg agggcgcggt gaaaattact
      301 aagcgtgctt tttcactcgc cgataaagtc ccggatatgg ttaaatcgat gatgcccgca
      361 ggcttcttga atctaaccga aaccgcgcgc ttcgacactg aaaccaaaac caaccgtttc
      421 gacgtgacat acgaacaaaa ccccgatcgg atgcagatta aaggcgaaac caagtatatc
      481 gacgaagggg aaaatagttc gcgccgtgag tataacatca ccgtcaaagt gaatatccct
      541 ctggtgggtt cccttttaga gatccaaatt gccagttctt tcaagaaagg cattgagaaa
      601 gattaccaaa acatccaaga tattttagct gctgaaaaat gaaacaaaac ataatagacc
      661 atcccccttt gggaattatt accaatccat acgcgggtaa aaacgccgcg attagccatc
      721 gcagcaaaga gttgcagcgt attatcggta aacacggtat tgtcaaagaa agccgcaact
      781 tagaccagct caaatcgata ctgtcagagt tcgcggcgca ccgtgtgccg tacattgttn
      841 nnnnnnnnnt gcgacatcac ttatgacccc ggccaatatg cggtttgcga tggcggcgac
      901 ggcacaatcc attggattgt caacgcctac ttagaagtgt tacaggagaa aaacgtggct
      961 gctctggagg ccgcgttccc catcctcatc cccaccaacg gcggcaccat cgattttctc
     1021 gcgcgcaaag tgggggcaaa gggaggcact tataaaattt tgcgcaagct gaggaaaacc
     1081 ttagacaaag gtcccctacc ccaaccggta acgctgcgca catttatgat ggaaggcact
     1141 tacaccaaag aacatttagg aaacgaatac caaaaattag cgttcgccgg agcgctcgcg
     1201 gggtttacac aaaaattttt taataaatac tatgaaagcg gcaaaccttc gttacaaaca
     1261 atagttgaag ttatcaccaa gacattcacc tcgacggcgt ttaaggggag ttggtttgaa
     1321 aattatgtac ccaaagatgt tataaattac tccaatgaaa tttttgatcc ctttgctgca
     1381 gaggtaaaag ttgacggtaa accgataccg tactcacgcc tcaacacatt gaacgttggc
     1441 tcaatcgcca tcgaaatcaa aaatttaatt aagcttttcg ccaacgccga ccgcgatcaa
     1501 aaactacata tgcatgtcgg ttatcttgag ccgtttgaag tcttctctaa cctcggtaat
     1561 ctttttgcag gcaccgatta ctcaggtaaa gagttagtcc aaaccaccgc
//
LOCUS       DAOMPD010000576         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_28769_length_1597_cov_2.861868, whole genome shotgun sequence.
ACCESSION   DAOMPD010000576 DAOMPD010000000
VERSION     DAOMPD010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28769_length_1597_cov_2.861868"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLY93_14355"
     CDS             <1..550
                     /locus_tag="PLY93_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRP70704.1"
                     /translation="IINPVPKKFQYRGLLYVGLMIASDKEDDVAVVEFNCRWGDPETQ
                     SILPLLEGDFLEYLLWTDGGNVEFPLLAYHAAKLIPYRRGAVVNVVIAAKGYPAAFLK
                     EIPLVFPKSKNNDLHILGAGVREENGVYFSTGGRIANVVAYAPTKEKARAAVYEYLRE
                     FEIANAAQREHLYWREDIAAGV"
     gene            550..1371
                     /locus_tag="PLY93_14360"
     CDS             550..1371
                     /locus_tag="PLY93_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70705.1"
                     /translation="MQTLGEILKHDDPAYLRYLSRKTQYLITREKSEYIHQFWRHLIV
                     EYHPQIVLPALSTADKKTFETLLKNFGHDENPTRRAKYQKLEKKIPWVFEHPNGGHFI
                     PYEIIKTLMPQQNLISDGYLFQLLYAMPKAEQNSLRALMAESQKARDTLTSEKHPLDR
                     ALALYIYSADQKHFRKPKRTPHARTLWQYLGYEFPKLENEIREWQYVMQNSQKGFYRS
                     LALVRSPHIHLLKSYTTSLWVVPVTTRRNRVFPAQNLRFVIPLEFLSAKNARASR"
     gene            complement(1322..>1597)
                     /locus_tag="PLY93_14365"
     CDS             complement(1322..>1597)
                     /locus_tag="PLY93_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP70706.1"
                     /translation="EKVTHENFDLIFMDIVMPELNGLEATAEIRKKEKEKGRPQVPIV
                     ALSGNAMSEDIQASKTVGCNAHLAKPVRKEELIEMLSRFLPTETPAV"
BASE COUNT          443 a          412 c          341 g          401 t
ORIGIN      
        1 aattattaac cctgtaccca aaaaattcca gtatcgggga ttgctttacg ttggcctcat
       61 gattgcctct gataaagaag atgatgtcgc cgtggtggaa tttaattgcc gctggggcga
      121 tcccgaaacg caatcgatac tcccccttct cgaaggcgat tttttagaat atcttttatg
      181 gacagacggt ggtaacgtcg aatttcctct acttgcctac catgccgcaa aactcattcc
      241 ctaccgacgt ggcgcagtgg tgaacgtcgt tatcgccgct aaaggatacc ctgcggcttt
      301 tctaaaagaa atcccacttg tttttcctaa aagcaaaaac aatgatttac acatcttagg
      361 tgcaggtgta cgcgaagaga acggtgtata cttttccact ggcggccgga ttgccaacgt
      421 cgtcgcgtat gcgccgacca aagaaaaagc gcgcgctgcc gtctacgaat acctccgcga
      481 gtttgagatc gccaacgcgg cgcagcgaga gcatttgtac tggcgtgaag acattgctgc
      541 aggcgtttaa tgcaaacgtt aggtgaaatt ttaaagcacg acgatcccgc ttatttacgt
      601 tatctctcac gaaaaacgca atacctcatc acgcgtgaaa aatcggaata catacaccaa
      661 ttctggcggc atctgatcgt cgaataccat ccacaaattg tgttgccagc gttaagcacg
      721 gcggataaaa aaacttttga aacgctgtta aaaaattttg gtcacgatga aaatcctaca
      781 agacgggcta aataccaaaa actcgaaaaa aaaatccctt gggttttcga gcaccccaac
      841 ggcggccatt ttatcccgta tgaaattatt aagactctca tgccgcaaca aaatctcatt
      901 agcgacggct atctttttca gctcctctac gcgatgccga aagcggaaca aaactcgtta
      961 cgcgcactca tggccgagtc gcaaaaagcg cgcgacacac taacgagcga gaaacaccca
     1021 ctcgaccgcg cgctcgcgct ttatatttat agcgccgatc aaaagcattt tcgaaaacca
     1081 aagcgcacac cacacgcccg cacgctttgg caatacctcg ggtatgaatt cccaaaatta
     1141 gaaaatgaaa ttcgggaatg gcaatacgtc atgcaaaata gccaaaaagg attttaccgt
     1201 tcgcttgcac tcgtgcgcag cccgcacata catctactca agtcgtacac gacttctctt
     1261 tgggtggtgc cggtgacgac acggcgcaat cgagtttttc cagcgcaaaa tttacgattc
     1321 gttataccgc tggagtttct gtcggcaaaa aacgcgagag catctcgata agttcttctt
     1381 tacgcaccgg ttttgcgagg tgcgcattgc aacccaccgt tttcgacgct tgaatatctt
     1441 cgctcattgc gttacccgaa agagcgacaa tcggcacttg cgggcgccct ttctcttttt
     1501 ctttcttacg gatttccgcc gtcgcttcta aaccattgag ttcgggcatg acgatatcca
     1561 taaaaataag atcaaaattt tcatgggtta ctttttc
//
LOCUS       DAOMPD010000577         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29296_length_1574_cov_2.416722, whole genome shotgun sequence.
ACCESSION   DAOMPD010000577 DAOMPD010000000
VERSION     DAOMPD010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29296_length_1574_cov_2.416722"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLY93_14370"
     CDS             <1..1246
                     /locus_tag="PLY93_14370"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70707.1"
                     /translation="WGLHEAAQKGVAIDQEILNKGVTYLSKELLSQELRKAADSYAQH
                     RLIFAQKVALALVANSKVKARIESDWKWYLEGGTREPAALALILEGSAKSSWKAISQS
                     ALEKLKALEIKSNVGSYFELTKKDNYYYWYNDREETTAQVLNALLTVDSKDNHALVER
                     AVAYLIAQKKQQRWRSTKLSALIMRAFSTYAQQSGEKLLPVKVTATVNGVEHTREYNP
                     KKSLAKDLLFTFNASGLNALINVSRSGRGFFLARVEWQHYLNAPLIKPKEGNFQLSRK
                     YFSVNKDGNRYSKGKMQYNFKAGDLVMTEVNLNAAKGNEYLLLEDMIPAGFEPLTTTE
                     LLDLGDLRYDRYANSPSATERLDDRVGLAKTYLYESSFTPRAFYRAVFPGKYHAMPAQ
                     GGLMYYPETYAYSTVDTITIED"
     gene            1381..>1574
                     /locus_tag="PLY93_14375"
     CDS             1381..>1574
                     /locus_tag="PLY93_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70708.1"
                     /translation="MKAYFLWISTRDGTLSQKAYHFLERKIPEAELFITSLKERYARF
                     PELPLAVCKKAEYSLFTPND"
BASE COUNT          465 a          343 c          358 g          408 t
ORIGIN      
        1 ttggggactt catgaagccg cacaaaaagg cgtagcgatt gaccaagaga ttttgaataa
       61 aggcgtcacg tacctttcta aggaattatt gtcgcaagaa ctcagaaaag cagcggatag
      121 ttatgcacaa caccgactga tttttgcgca aaaagttgca ctggctttag tggctaactc
      181 taaagtcaaa gcgcggattg agtccgattg gaaatggtat ttagaagggg gcacgcgcga
      241 acccgctgcg cttgcgctta ttttagaagg ctccgcaaag tcctcgtgga aagcgataag
      301 ccaaagcgca cttgaaaaat tgaaagccct tgaaataaaa tcgaatgtcg gttcgtattt
      361 tgagcttaca aaaaaggata actattatta ttggtacaac gaccgtgaag aaacaacggc
      421 gcaagtgttg aacgcacttt taacggtgga ttcaaaagac aaccatgctt tagtcgaacg
      481 cgccgtggcg tatctcattg cgcaaaaaaa acaacaacgt tggcgtagta caaaactttc
      541 agcgcttatc atgcgcgcat tttccactta tgcacagcaa agcggcgaaa aattattgcc
      601 ggtaaaagtg actgcaactg ttaacggtgt tgagcacacg cgcgagtaca accccaagaa
      661 atcactcgcc aaagatttgc tgtttacgtt taatgcgagc ggcttaaacg cattgatcaa
      721 tgtgtcgcgt agcggtagag gtttcttcct tgcgcgtgtc gagtggcaac attacttgaa
      781 tgctcctctc attaaaccca aagagggaaa ttttcagttg agtcgcaaat actttagtgt
      841 gaataaagat ggcaaccgtt actccaaagg aaaaatgcaa tacaatttca aagcgggcga
      901 tttggtgatg accgaggtga acttgaacgc ggcgaaaggg aacgaatacc ttcttctcga
      961 agacatgatt cctgccggct ttgaaccgct caccacgact gaacttttag atttgggaga
     1021 tctccgctac gaccgctacg ccaatagccc ttcggcgaca gaacgtctcg acgaccgcgt
     1081 tggccttgcg aaaacctatc tttacgaatc gtcgtttacg ccgcgcgctt tttatcgtgc
     1141 ggtttttccg ggtaagtacc acgcgatgcc tgcacaaggc ggtctcatgt actatcctga
     1201 aacctatgcg tattcgactg tagacacgat taccatcgaa gattaaaagg tgaattaaag
     1261 ccgaaaaaga aaaacatagt gagcatgatt tcttacattt tcataaggcg tgtatgttta
     1321 ttgtgcttgt tgcttttctt tgcgcacacc tctctctata ccaaacctaa ggaaataaaa
     1381 atgaaagctt attttttgtg gatctcaaca cgcgatggga cgctttcaca aaaagcatat
     1441 cattttttag aaagaaaaat tccagaggcg gagcttttta tcacttcatt gaaggagcgg
     1501 tatgcgcgct ttccggagct tccgctcgct gtatgtaaaa aagcagagta ttcactcttt
     1561 accccaaatg attt
//
LOCUS       DAOMPD010000578         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29328_length_1573_cov_2.667984, whole genome shotgun sequence.
ACCESSION   DAOMPD010000578 DAOMPD010000000
VERSION     DAOMPD010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29328_length_1573_cov_2.667984"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLY93_14380"
     CDS             complement(<1..244)
                     /locus_tag="PLY93_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70709.1"
                     /translation="MRGIISLLVLTFIACKKPPVVVGPCDEDLGFATKHEHLANVQFL
                     TETKQVDWFEIYDNLTLRHKNIAPKKCIPTEQNKSIE"
     gene            447..791
                     /locus_tag="PLY93_14385"
     CDS             447..791
                     /locus_tag="PLY93_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70710.1"
                     /translation="MRQHKLFAVAALVAVAVFSTACGSAKIPDDSPDIFPSSVEKSQA
                     KAAIDDAQNAYNALPDDGSGEKRDAKSFLDKAKEYLSDGEPEAAWSAATKSKSFSSLA
                     AEARKVRAAGLQ"
     gene            803..1501
                     /locus_tag="PLY93_14390"
     CDS             803..1501
                     /locus_tag="PLY93_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70711.1"
                     /translation="MKKIFSTILIAAFLSPVFAGVIKESQAKREVDDAIEALTAMDAR
                     TDLATFLPQREAYAARTYHNKARNFLADGDYDKASFYAILSTNFSKISVYRGLLAKAD
                     RDKLERAAQGAVVPSLKTAGLKGKGKSDIFAGSYTLKTVYNVRSVWRVDKIPALHEGM
                     IEKLTTIASVMKQQGDVKMNIETKARNEEISEKYAKDIKDFMIEKGIDTSRIEYVNKK
                     GRDSIEITLSGVKH"
BASE COUNT          493 a          313 c          350 g          417 t
ORIGIN      
        1 gttcaatcga tttattttgc tcggtgggga tacacttctt cggtgcgatg tttttatggc
       61 gtaacgttag attgtcgtaa atttcaaacc aatcgacttg tttggtttcg gttaaaaatt
      121 gcacattggc taagtgttca tgttttgtcg caaagcccaa atcttcgtcg caggggccaa
      181 ctaccacggg aggtttctta caagcgataa aagtaagcac taatagagaa atgattccac
      241 gcatagtata caagtatcga caaagtgcgc tttattgtca attgcttttg ccttgacgta
      301 cgtaatctta ccttgttttt ggaaagtaag tacgcatatt tttgcgaacc aaaagtcgta
      361 atgtaaggtc tactaaatgg ggacaaatgg ttacatatat cccgcgttag atgttacaaa
      421 gacttaagaa caattaggag atttacatga gacaacacaa actatttgct gttgccgcac
      481 tcgtggcagt tgctgttttt agtaccgcct gtggtagcgc aaaaattcca gacgattcac
      541 ccgatatttt cccatcgagt gtagagaaaa gccaggcgaa ggcagcaatc gacgatgcgc
      601 agaatgcata taatgcattg cccgacgacg gtagcggcga aaaacgcgac gctaaatcgt
      661 ttttagataa agcgaaagaa tatttaagcg acggcgaacc cgaagcggca tggtctgcgg
      721 cgactaaatc aaaatctttt tcatctctgg ctgcagaagc gcgtaaagtt cgggcagcag
      781 ggcttcaata aggagtagta caatgaagaa aatattttca acaattctta tagcggcatt
      841 tttgtcgcct gtatttgctg gcgtcattaa agaaagccaa gcaaaacgcg aagtcgacga
      901 cgctattgaa gcattgactg cgatggacgc acgcaccgat cttgcaacat ttttaccgca
      961 acgtgaagcg tatgcagcgc gcacatacca caataaagcg cgtaattttt tagcagatgg
     1021 cgattacgac aaagcatcgt tttatgcgat tctttcgacg aatttttcaa aaattagcgt
     1081 ttaccgaggt ttacttgcta aagcagaccg cgataaactc gaacgtgcag cgcaaggcgc
     1141 agttgtgccc agccttaaaa ccgctggtct taaaggaaaa ggcaagagcg atatttttgc
     1201 cggttcatac acattaaaaa cggtctataa cgtccgcagc gtttggcgtg tagataaaat
     1261 tccagcgctc catgagggga tgattgaaaa actcaccacc attgctagcg tcatgaagca
     1321 acaaggcgat gttaaaatga atatcgaaac caaagcgcgc aatgaagaaa tttcagagaa
     1381 atacgcaaaa gatatcaaag actttatgat agaaaagggc attgatactt ctcgtatcga
     1441 atacgtgaat aaaaaaggcc gtgatagtat agaaattact ctctctggcg ttaaacatta
     1501 aaacatataa taggggatgt ctaattggct gctggtacag ccattagaca atccttattt
     1561 tttcaaccaa ata
//
LOCUS       DAOMPD010000579         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29506_length_1565_cov_2.379470, whole genome shotgun sequence.
ACCESSION   DAOMPD010000579 DAOMPD010000000
VERSION     DAOMPD010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29506_length_1565_cov_2.379470"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLY93_14395"
     CDS             complement(<1..965)
                     /locus_tag="PLY93_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRP70712.1"
                     /translation="MENTKKNSADLTFYIETLGCPKNRADSRRMRTSFLSLGYKEAEK
                     PEMADFFLINSCSFIREAQEETISTVFDALKIKENKSPNMKVGLIGCFVERFSDAVKS
                     DIPELDFTIGTQRYHEVGGLISEKFEITTTGHAALENQKISSNADSQKPFAWLRIAQG
                     CNRHCAFCIIPTIRGPLKPYELTTVEKQLDDEKALRGSEAIPIREVILVSQDTISQGV
                     DAIERIVANLSEKEEVAWIRLQYLFPDRNILRLLKLWEKYPKLTPYLDIPFQHTSPTI
                     LKAMKRPSDVGLFTEIIERAHAIHPDFEIRTSFIVGYPGEGVEEFS"
     gene            complement(987..>1565)
                     /locus_tag="PLY93_14400"
     CDS             complement(987..>1565)
                     /locus_tag="PLY93_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70713.1"
                     /translation="ISFSIQNTEVFIVLRDISSDEKTGAYKANVELFPGKKKYILKEE
                     VPVYMEDSAIPRKFKVTLSGITATNAKIVIDLGEEIQATENETPSDVRVSNPDNFLIR
                     LEVLLTGDTYIEYFVDGKPGKRGLLKKDTQLLLEANDSIQIKIGDAGDVKLMLNGKSE
                     VLGQKGRVANKIIRKVRDPVEQSKFKILIKDA"
BASE COUNT          373 a          322 c          392 g          478 t
ORIGIN      
        1 gaaaattcct ccacgccttc gcctgggtag cctacaataa atgaagtacg gatttcaaaa
       61 tcaggatgga tggcgtgcgc acgctcgata atttctgtaa aaagaccaac gtccgaagga
      121 cgtttcatcg ctttcaggat tgtgggggag gtgtgttgaa aagggatgtc gaggtagggg
      181 gtcagttttg gatatttttc ccaaagcttt aaaaggcgca aaatatttct gtcgggaaaa
      241 agatattgta agcgtatcca cgccacctct tctttttcag aaagatttgc cactatgcgt
      301 tcgatggcgt cgaccccttg gctaatggtg tcttggctga ctaaaataac ttcgcggatg
      361 ggaatggctt cggaaccgcg cagggctttt tcgtcgtcca gttgtttttc gacggttgtg
      421 agttcatagg gttttaatgg gccacggatg gtggggatga tacagaacgc acaatgacgg
      481 ttgcagcctt gggcaatgcg cagccacgca aacggttttt gcgaatcggc gttggaggaa
      541 attttttgat tctctaaagc tgcgtgaccg gtggtggtga tctcaaactt ttcgctgatg
      601 agaccaccta cctcgtggta acgttgcgta ccgatggtga aatcgagttc ggggatgtcg
      661 cttttgacgg cgtcggagaa gcgttcgaca aagcagccga taagacccac tttcatgttg
      721 ggtgatttat tttctttaat ttttaacgca tcgaagacgg tggaaatcgt ttcctcttgt
      781 gcttcacgga taaaagagca actattgatg agaaaaaaat ctgccatttc aggcttttct
      841 gcttctttat atcccaagga gagaaatgac gtgcgcatcc gccgcgaatc ggcgcggttt
      901 ttagggcagc ccaaagtttc gatatagaac gttaaatctg ctgagttttt ttttgtattt
      961 tccacttttt taattaaagc gtacctttaa gcgtctttaa tgagaatctt aaattttgat
     1021 tgctcgacgg gatcgcgtac tttacggata attttattgg cgacgcggcc tttctggcct
     1081 aaaacttcgc ttttcccgtt gagcataagt ttgacatccc ccgcatcgcc aattttaatt
     1141 tggatactgt cgttcgcttc gaggagtaat tgcgtgtctt ttttaagcaa gccacgttta
     1201 ccgggtttgc cgtcgacaaa atactcgata tacgtgtcgc cagtgagcag cacttcgagg
     1261 cggattaaaa agttatcggg gttggagacg cgaacgtcgc tgggagtttc gttttcggta
     1321 gcttggattt cttcgcccaa gtcgatcaca attttagcgt tagtcgcggt gatgcccgat
     1381 aaagtaactt tgaacttacg cggtattgcc gaatcttcca tatatacagg aacttcttct
     1441 ttgaggatgt atttcttttt ccccggaaag agctctacgt tggctttata cgcgcctgtt
     1501 ttctcgtcgg aactgatgtc gcgcagtaca ataaaaacct ctgtgttttg gattgaaaaa
     1561 ctaat
//
LOCUS       DAOMPD010000580         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29678_length_1557_cov_2.605193, whole genome shotgun sequence.
ACCESSION   DAOMPD010000580 DAOMPD010000000
VERSION     DAOMPD010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29678_length_1557_cov_2.605193"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLY93_14405"
     CDS             <1..690
                     /locus_tag="PLY93_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70714.1"
                     /translation="VLALMTPAVYADSLKAEKPFVPTSHYVKQTMEGWSVRVNKDLLN
                     GIDKQTGTRALALLKNKLSEIRRIVPRQALAKLMQVSIWLGVDDYAIPNATYHPSREW
                     LQENGWNSDKARGVEISNAKIFLEWSQDQPMLVLHELSHAYHDQVLGYDNAELERCYA
                     RAKKSGKYNRVKNISGAVVRAYALNDAHEFFAEASEAYFGKNDFQPFTRSELKKFDLD
                     TYRVIEKMWNR"
     gene            complement(887..1249)
                     /locus_tag="PLY93_14410"
     CDS             complement(887..1249)
                     /locus_tag="PLY93_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HRP70715.1"
                     /translation="MKKIILILAVSIFAATSLSAKGECRSDREKLCSSVKKGDHKAMW
                     QCMKEHETELSDACKNKISQVREKNREVKKACKADYKQFCKDKKAGKGHIIHCLKKNE
                     AQLSESCKNALSQPVKVD"
BASE COUNT          428 a          374 c          332 g          423 t
ORIGIN      
        1 gtccttgcgc tgatgacgcc cgcagtgtac gcagattcgc tgaaggcgga aaaaccgttc
       61 gtgccaacat cgcattacgt caaacaaaca atggaaggct ggtcggtgcg ggtaaacaaa
      121 gatttgttga acggaataga caagcaaacc ggaacacgtg cgctcgctct cctaaaaaac
      181 aaactttccg aaatacggcg catcgtacca cggcaggcgc tcgctaaatt aatgcaggtc
      241 tcgatttggc tgggtgtcga cgattatgcc atacccaacg caacctacca ccccagccga
      301 gaatggctcc aagagaacgg ctggaacagc gacaaagcgc gcggagttga gatctccaac
      361 gcaaagattt ttctcgaatg gagtcaagac caacccatgc tcgttttgca cgaactctcg
      421 catgcatacc acgaccaggt tttgggctac gacaatgccg agctcgaacg ctgctatgcg
      481 cgtgcaaaaa agagcggcaa atataaccga gtcaagaaca tatcgggtgc agtcgtgcgt
      541 gcgtatgcgc tgaacgatgc gcatgagttt tttgccgagg catcggaagc ttattttggt
      601 aaaaatgatt ttcagccatt cacgcgcagc gagctcaaga aattcgacct tgatacatac
      661 cgcgtcatcg aaaaaatgtg gaatagataa agcacctcga aaaaggtcgc aaagaaatca
      721 gaaacaccgc agtttgaaga caaactgctt gcaaagcggc ttaatcaacc tgtttccaat
      781 tttctgtgcc gccatgcgga cggtattcac ggatttttcg aggttactat aagtatattc
      841 agtacggagt aggtagtccc ccactccgcg tgattagcga gaaaaactaa tcaactttta
      901 caggttgtga caaagcgttc ttacaacttt cgctgagttg tgcttcattc tttttcaaac
      961 aatgaatgat gtgtccttta cccgcttttt tgtctttaca gaattgctta taatctgcct
     1021 tacaagcttt ttttacttca cggttttttt cgcggacttg agaaatttta tttttacacg
     1081 catccgaaag ttcagtttcg tgttccttca tacactgcca cattgcttta tgatcgcctt
     1141 ttttaacact cgagcaaagt ttttctcgat cacttcggca ctcacctttg gctgaaagcg
     1201 atgtagcggc aaaaatcgat actgctaaaa taagaatgat tttcttcatg tttactcctt
     1261 aatgtcgagg tatatcccct ttgataggac gtatctacaa aagattacgg acacaaaatc
     1321 caccataagc tcgaagagtt tatgcactaa gagcctgact tcgtgtcgat atctgctatt
     1381 tcaaaatttt tgcatcgtcg gtattttatc gctgtggtgg ttcaacgacc cccgcacttt
     1441 ttttacccat tcgtaatatt tcaagtccgg tggcgcacgg tgtaccgaat ctacaaacgt
     1501 ataattttct tcttttttga gtagcagatg ccccagctcg tgcaaccaca cccacgc
//
LOCUS       DAOMPD010000581         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29686_length_1557_cov_2.084554, whole genome shotgun sequence.
ACCESSION   DAOMPD010000581 DAOMPD010000000
VERSION     DAOMPD010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29686_length_1557_cov_2.084554"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLY93_14415"
     CDS             <1..209
                     /locus_tag="PLY93_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRP70716.1"
                     /translation="NDVAKVDYCVHTLKGSAMSLGFAAMSAILIDLNNRTKVKNLDGA
                     SADLDKLEALLAEVKVYKEANFPG"
     gene            complement(206..580)
                     /locus_tag="PLY93_14420"
     CDS             complement(206..580)
                     /locus_tag="PLY93_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRP70717.1"
                     /translation="METNKKAMIVDDSRLTQRQLAQILTDCGIDVTDIAANGLEAVDK
                     VKTNPNAFDLITLDITMPQLDGVSALKEIMQVNPNARVLMVSAIGKENVIKACMASGA
                     RNFIVKPFQAEKVKEIVKATLA"
     gene            complement(602..>1557)
                     /gene="cheB"
                     /locus_tag="PLY93_14425"
     CDS             complement(602..>1557)
                     /gene="cheB"
                     /locus_tag="PLY93_14425"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HRP70718.1"
                     /translation="LPDVLIVDAEMPRMGGLHFLRIIMAQKPLPIIMCATHSTHGKAL
                     LREALARGAANTIEKPKLALRRFFDKYADEIIQRVRDASTQKTALRRRRILVPKGEYV
                     SPPPRYTADAVLSIEKGGSLTTTDTIIAVGASTGGIEALKVFIEALPLETPGVVVVQH
                     MPEKFTGSFSARLDDISTVNVKEAENGDKLERGKVLIAPGNKHLLVKRRGHHYYVEIV
                     SGDLVNRHRPSVDVLFRSVANVAGKNAIGVIMTGMGDDGARGMREMKNNGAYNIAQDE
                     KTCVVYGMPKEAVEHGAVDIILPLESIAKAVLKRAALMEKK"
BASE COUNT          347 a          402 c          392 g          416 t
ORIGIN      
        1 caaacgacgt ggcaaaagtc gactattgcg tgcacacgct caaaggtagc gcaatgagtt
       61 tgggctttgc cgccatgagc gcaattctca tcgacttaaa caaccgcacc aaagtaaaaa
      121 atttagacgg cgccagcgcc gatttagaca agctcgaagc acttttagct gaagtgaagg
      181 tctataaaga ggcgaatttt cctggttaag ccaacgtcgc cttcacaatc tccttcactt
      241 tttctgcttg gaatggcttc acaataaagt tacgcgcacc cgaggccata caggctttaa
      301 tcacattctc tttaccgatg gcgctcacca tgagtacgcg tgcgttgggg ttgacttgca
      361 taatctcttt gagcgcagag acgccgtcga gctgcggcat ggtgatgtcg agagtgatga
      421 gatcgaaagc gttggggttg gttttcactt tgtcgaccgc ttccaagcca tttgcggcaa
      481 tgtcggtaac atcgataccg caatcggtta aaatttgtgc cagttgccgc tgcgttaacc
      541 gcgagtcgtc gacaatcatt gcttttttgt tggtttccat agtttcttcc ttttagttat
      601 gctatttttt ttccataaga gcggctcttt tgagcactgc ttttgcaatg ctttcgagcg
      661 gcaggataat atcgacggca ccgtgttcca ccgcttcctt gggcattccg tacaccacgc
      721 acgttttttc gtcttgcgca atgttatacg cgccgttgtt tttcatttcg cgcatgccac
      781 gcgcgccgtc gtcgcccata cctgtcataa taacgccaat ggcattttta ccagccacgt
      841 tggcgaccga acgaaaaagc acatcgaccg aagggcggtg gcgattcact aaatcgccgc
      901 ttacaatttc gacatagtag tggtggccgc gccgtttcac cagcaagtgc ttattcccag
      961 gggcaatgag tactttccct cgttcaagtt tatcgccgtt ttcagcttct ttcacattga
     1021 ccgtgctgat gtcgtcaaga cgcgcagaaa acgagccggt gaatttttcg ggcatgtgtt
     1081 gcactacaac cacgccaggc gtctccagcg gcaacgcctc tataaacact ttcagtgctt
     1141 cgataccgcc ggtcgacgca ccgacggcga taatggtgtc ggtggtggtg agcgatcccc
     1201 ctttttctat agaaagcact gcgtcggcag tataacgcgg gggcgggctt acatactcgc
     1261 ctttaggtac aagtattcta cggcggcgca gcgctgtttt ttgggttgaa gcgtctcgta
     1321 cacgttggat aatttcatcg gcgtatttgt caaagaagcg gcggagcgca agtttaggtt
     1381 tttcaatcgt gttggcagcg ccgcgtgcca acgcttctcg aagtaacgcc ttcccgtgcg
     1441 tggaatgggt cgcgcacatg ataattggca ggggcttttg cgccataata atacgcaaaa
     1501 agtgcaatcc ccccatacgc ggcatttcag cgtcgacaat gaggacatcg ggcaagc
//
LOCUS       DAOMPD010000582         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29691_length_1557_cov_1.921438, whole genome shotgun sequence.
ACCESSION   DAOMPD010000582 DAOMPD010000000
VERSION     DAOMPD010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29691_length_1557_cov_1.921438"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>425
                     /locus_tag="PLY93_14430"
     CDS             <1..>425
                     /locus_tag="PLY93_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRP70719.1"
                     /translation="FLRAIGADVRLVAPAPFSNPNNYYHIARKTAETIPGAFWANQFE
                     NTSNADAHYLGTGPELWRDTDGKMDIFVTSAGTGGTIGGITKFLAEKNPALESYLVDP
                     TGSGLYTYINTGDFKAEGGSITEGIGINRLTQNFAPAKL"
     assembly_gap    426..435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <436..695
                     /locus_tag="PLY93_14435"
     CDS             <436..695
                     /locus_tag="PLY93_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70720.1"
                     /translation="AYDQEVIDLCHHLTKHEGLFVGSSAALNVAGALRLAKNAKVGST
                     LVTILCDGGGRYQSRLYNDAWLKEKNFIPRTVDAAWLSSLT"
     gene            complement(746..>1314)
                     /locus_tag="PLY93_14440"
     CDS             complement(746..>1314)
                     /locus_tag="PLY93_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRP70721.1"
                     /translation="IKFWDVFGNFAPFGGVNIAYIIRARVIWLRDLGFAMSPFNAWLF
                     VQGLETLALRQRQHSANALAVAQHLKKHPKVSWVNYPGLTDSPNHALAKQYLGEHFGA
                     LVGFGVKGGLEAGKKFINSVKLLSHLANIGDAKTLVIHPASTTHSQLTEAEQIETGVS
                     PDYVRLSVGLETVSDITADIDQALAQVE"
     assembly_gap    1315..1324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1325..>1557)
                     /locus_tag="PLY93_14445"
     CDS             complement(<1325..>1557)
                     /locus_tag="PLY93_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRP70722.1"
                     /translation="KAISKIAHDAGVPLIVDNTVGVGLVRPIEHGADIITMAATKFVG
                     GHGTSIGGVIVDSGKFAWNNGKYPDYTEPDAGY"
BASE COUNT          352 a          383 c          416 g          386 t
ORIGIN      
        1 tttttacgcg cgattggcgc cgatgtgcgc ctcgttgccc ctgcgccttt ttcaaatccg
       61 aataactact accacattgc gcgcaaaacc gccgaaacaa taccgggagc gttttgggca
      121 aaccaatttg aaaatacatc gaacgcggat gcgcactact tgggtaccgg gcccgagttg
      181 tggcgtgaca ccgatggtaa gatggacatc tttgttacct ccgcgggcac gggcgggacc
      241 attggtggga ttaccaaatt tcttgctgaa aaaaatccag cgcttgagtc ataccttgtc
      301 gatcccaccg gttcgggcct ctatacgtat atcaacaccg gtgatttcaa agccgagggc
      361 ggttctatca ccgaagggat tggtatcaac cgtttgactc aaaattttgc tccggcgaaa
      421 cttaannnnn nnnnngcgcg tacgatcaag aggtgattga tctttgccac catcttacaa
      481 agcacgaagg tctttttgtc ggcagctctg ccgcgttgaa tgtggcggga gcgttgcgct
      541 tggccaaaaa tgccaaagtg ggatcgactc ttgtcacgat tttatgcgac ggcggtggaa
      601 gataccagag tcgtctttac aacgacgctt ggctcaaaga aaaaaatttt attccccgca
      661 ctgtcgacgc cgcatggttg tcgtctttaa cataaaaaaa tgccgcgcaa tgcgcggcat
      721 ttcgctttgt agcggcttta tttctttact caacttgagc tagtgcttgg tcgatatcgg
      781 cagtgatgtc ggataccgtt tcaagaccga cagacaagcg tacgtaatcg gggcttactc
      841 cggtttctat ttgctctgct tcggtgagtt gcgagtgtgt ggtcgatgca gggtggatga
      901 ccaacgtctt tgcatcgccg atgttcgcta ggtgggacaa cagctttacc gagttgataa
      961 actttttacc tgcttcaaga ccgcctttga caccaaagcc gacaagcgcg ccaaagtgct
     1021 cgcccaagta ctgctttgcg agcgcgtggt tagggctatc tgtaagaccc gggtagttga
     1081 cccacgatac ctttgggtgt ttcttcaagt gttgtgcgac ggcgagtgcg ttggcgctgt
     1141 gttggcgttg gcgcagagcg agcgtttcaa gaccttgtac aaaaagccat gcgttaaaag
     1201 ggctcattgc aaaacctaag tcgcgcagcc aaattacgcg cgcccgaata atgtacgcga
     1261 tattcacgcc gccaaaaggc gcgaagttac cgaagacatc ccaaaattta atgcnnnnnn
     1321 nnnnatagcc tgcgtcgggt tcggtgtagt cggggtattt gccgttgttc cacgcaaatt
     1381 tacccgagtc gacaatcacg ccgccgatgg atgtaccgtg gccgccgacg aattttgtgg
     1441 cagccatggt gataatgtcg gcgccgtgtt caatggggcg cacgaggcct acgcccacgg
     1501 tattgtcgac gatgagaggc acgccggcgt cgtgtgcaat tttagaaatc gctttga
//
LOCUS       DAOMPD010000583         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29700_length_1556_cov_2.646902, whole genome shotgun sequence.
ACCESSION   DAOMPD010000583 DAOMPD010000000
VERSION     DAOMPD010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29700_length_1556_cov_2.646902"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /gene="yihA"
                     /locus_tag="PLY93_14450"
     CDS             <1..595
                     /gene="yihA"
                     /locus_tag="PLY93_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRP70723.1"
                     /translation="SKTIFNDTYRLRAEYLGKFNDVKLMPPTIAEIAFIGRSNSGKSS
                     LLRAFLNSDKMPKVSSKPGSTRLIHLYRIGEEGVTLADFPGYGYAQSAKAYRARFIAM
                     LNNYLKSPRAIKALCLLMDARRTLGEDEELLVQIAIERKIPIILCLNKSDQLNQKEVA
                     QLKKKFAVTKPFFEVLLVSAEKRVNLDYLRSFILSLS"
     gene            complement(645..>1556)
                     /locus_tag="PLY93_14455"
     CDS             complement(645..>1556)
                     /locus_tag="PLY93_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRP70724.1"
                     /translation="RHRKNKYTLQLKTEYTTLVREVAAYRFDIAIFSYAEKHSARLIQ
                     DAGIPYRLGPLRRSFFTRFNLWYYINRKKSNQAEYQLNLKMLRSLRLQNTFQFPRVNI
                     PSLSAPSIKDRYIVIHPYKRSGTALVWPIENFQNLARYFTQQKIRVVILGDQSDSEVL
                     HSHFEDISLVEIRTDLSLVQSAALIRGALHFFGNSSGPLHLAALVGTPHTGFYPQNRA
                     SSARRWRTLPLASAPFASQYLLNTNFPMDVQKCIGKKCKFYPCTVNISLEAAIQSTVA
                     WKSFNKKSSKKKIQKTTKKKVQRKNAS"
BASE COUNT          413 a          332 c          378 g          433 t
ORIGIN      
        1 ttcaaaaact attttcaacg acacttatag gttgcgtgcc gagtatctgg gtaaattcaa
       61 cgatgtaaaa cttatgccgc ccaccatagc tgagattgca ttcattggtc gctcgaacag
      121 cggcaaatcg tcgcttttac gcgcatttct taacagcgat aaaatgccga aagtttcctc
      181 gaagccggga agcacccgtc tcatccatct ttaccgcatt ggcgaagaag gagttacctt
      241 ggcggatttt cccgggtatg ggtatgcgca gagcgctaaa gcgtatcgag cgcgctttat
      301 cgcgatgctc aacaactatc ttaaatcacc acgcgctata aaggcgctct gcctgctgat
      361 ggacgcgcga cgcaccttgg gtgaagacga agagttattg gtacaaatcg caatcgaaag
      421 gaaaattccc ataattttat gcctcaataa atcagatcag ctcaatcaaa aagaagtggc
      481 gcagctcaaa aaaaaatttg cagtgacaaa accgttcttt gaagtgttgc tcgtgtcggc
      541 agagaagcgc gtgaatttag attatttacg gagctttatt ttatctttaa gctaagattg
      601 gcaacttact gagatttgat ttaaagtact cgttgctaaa ctccctaaga agcatttttc
      661 cgttggactt tttttttcgt tgtcttttgt atttttttct tggaggattt tttattgaag
      721 ctcttccacg ccaccgtcga ttgaatggcc gcctcaagag aaatatttac ggtacaggga
      781 taaaatttac atttttttcc gatacacttt tgcacgtcca tgggaaagtt ggtgttgagt
      841 aaatattgcg aagcaaaagg cgcgctggct aaagggaggg tgcgccaccg ccgtgccgac
      901 gatgcgcggt tttgtggata aaaacctgta tgtggtgtgc cgacgagcgc cgctaaatgg
      961 agtggcccgg acgagttgcc aaaaaaatgg agcgcgcctc ggatgagcgc agcgctttgc
     1021 actaaactta aatcggtacg gatttccact aaagatatat cctcaaaatg cgaatgcaac
     1081 acttcgctat cgctttgatc gccgagaatc acgacgcgaa ttttttgctg cgtgaaatac
     1141 cgcgctaagt tttgaaaatt ttctatcggc cacacgagcg cagtccccga gcgtttgtat
     1201 ggatggatga cgatgtagcg gtcttttatc gaaggagcgc tcaaagaagg aatgttgact
     1261 cgcgggaatt ggaatgtgtt ttgcaaacgc aacgagcgca gcattttcaa attgagttga
     1321 tattccgctt gattactttt ttttcggttg atataatacc ataaattaaa gcgcgtaaaa
     1381 aaggaacgcc gcaaaggtcc caagcggtac ggtatacccg cgtcttgaat gaggcgtgcg
     1441 ctgtgctttt cggcgtacga aaaaatagcg atgtcgaatc ggtatgccgc cacttcgcgc
     1501 accagcgtgg tgtattcggt cttcagttgt aacgtgtatt tatttttacg gtggcg
//
LOCUS       DAOMPD010000584         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29744_length_1554_cov_2.929953, whole genome shotgun sequence.
ACCESSION   DAOMPD010000584 DAOMPD010000000
VERSION     DAOMPD010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29744_length_1554_cov_2.929953"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..417
                     /gene="rplU"
                     /locus_tag="PLY93_14460"
     CDS             106..417
                     /gene="rplU"
                     /locus_tag="PLY93_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRP70725.1"
                     /translation="MQAVIQVGSKQYKVAPDTVFFAELTGVEPGKDFNTNQVLSVNNN
                     GQVKIGQPFVSGASVTAKVLEEVKGPKIIGVFYRTKKHNHRRWGHRQKYHKLQVTSIQ
                     A"
     gene            complement(421..1215)
                     /locus_tag="PLY93_14465"
     CDS             complement(421..1215)
                     /locus_tag="PLY93_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70726.1"
                     /translation="MLRKYLPQLFILSTAALLAIVGYVLVDLNKNSLGVVSITTYIGI
                     VVMAVAMKIAGYLLGDKVPHANGKKYGDVMDWAMLLFVWPSYVTPLVVVPHLVGHTLP
                     IFALFIVGIGLFATLSLVGLQISANRINGKRRYATWLYFIPAFLYIIPALIIGYFIVG
                     KFSTSLTVMRFANIGGLLLGAFFSFYCVKIANSDLFDYDNRRYRFFDLAPLFFVVIAV
                     AIIAEFALPLFQGRAHEMYLLYTMELYTLIVAQVLGVLSVTRRAKF"
     gene            complement(1215..>1554)
                     /locus_tag="PLY93_14470"
     CDS             complement(1215..>1554)
                     /locus_tag="PLY93_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70727.1"
                     /translation="IMKMNGNNIQGIELKYEETALRRQFSHKRSIKNDDISKTSYGHI
                     SLYYHAEGDTPKVETLANMSTDDRAKLLAIYIDGLLRGIRDLDNRVLAAKKFAGKKMS
                     DALNMGVRVR"
BASE COUNT          461 a          350 c          350 g          393 t
ORIGIN      
        1 aatacccccc aattgcggcg tagcatttgt caggtatgtc cgtgggttag acacacaagt
       61 tcgcaataac cgttgaccac ctgtctctat cctccatttt gacgaatgca ggctgttatt
      121 caagtgggta gtaaacaata taaagtcgcc cccgacaccg ttttttttgc tgagttgaca
      181 ggagttgaac caggtaaaga ttttaataca aatcaagtcc tttctgtcaa taataacggc
      241 caagtaaaaa ttggtcaacc ctttgtatct ggcgcgtctg tcaccgccaa ggttttagaa
      301 gaagttaaag gccctaaaat tattggtgta ttttaccgta ccaaaaagca caaccaccgt
      361 cgttggggtc atcgccaaaa gtaccataaa ctacaagtaa cgtccatcca agcttaaccg
      421 ctaaaattta gcgcgccgag ttaccgagag aacccccaac acttgcgcca cgataagggt
      481 gtatagttcc attgtataaa gaagatacat ttcgtgcgct ctcccttgaa aaagagggag
      541 tgcaaactct gcgataatag ccacggcaat cactacaaaa aagaggggcg ctaaatcgaa
      601 aaaccgatag cggcggttgt cataatcgaa taaatcagag ttagcaatct ttacgcaata
      661 aaagctaaaa aaagctccca aaagaaggcc gccgatattt gcaaaacgca tgacggttag
      721 gcttgtcgaa aatttaccaa cgatgaagta accaataatg agagccggta ttatataaag
      781 aaaggctggt ataaaataga gccaagttgc gtaacggcgt ttgccgttga tgcggtttgc
      841 gcttatttgt aaacccacca aagaaagagt cgcgaataaa ccaatcccca caataaagag
      901 agcaaaaata ggtaatgtgt gtccaacgag atgcggaacg acaacgagcg gggttacata
      961 gctcggccat acgaaaagga gcattgccca atccataaca tcgccgtatt tttttccgtt
     1021 ggcgtgcggt actttatcgc ctaaaagata gcctgcaatt ttcatcgcca ccgccatcac
     1081 aacgatgccg atgtatgtgg tgatcgacac tacccccaat gaatttttat ttaagtcaac
     1141 gaggacatag ccaacgatgg cgagcaacgc ggcagtgctt aagataaaaa gttgaggtag
     1201 gtatttgcgt agcattatcg aacacgaacc cccatgttca gagcgtcgct catttttttg
     1261 cctgcaaatt ttttagccgc caacacacgg ttatccaagt cgcggatgcc gcgcaaaagg
     1321 ccgtcgatat agatggccag caatttagct ctgtcgtcgg tgctcatgtt agcgagcgtt
     1381 tccactttag gggtgtctcc ctcggcgtga tagtaaaggc taatatggcc atagctggtt
     1441 ttactgatgt cgtcgttctt gatggaacgt ttgtgcgaga attgacggcg cagagcggtc
     1501 tcttcgtact tgagctcaat tccttgaatg ttgttgccat tcattttcat gatg
//
LOCUS       DAOMPD010000585         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_29923_length_1546_cov_3.197854, whole genome shotgun sequence.
ACCESSION   DAOMPD010000585 DAOMPD010000000
VERSION     DAOMPD010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29923_length_1546_cov_3.197854"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLY93_14475"
     CDS             <1..851
                     /locus_tag="PLY93_14475"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRP70728.1"
                     /translation="LVSATLPEASKRYQWPLKNFLHRVYAPMRQIFAINEEHAERLRR
                     ISPLNVSIAGDTRFDAIRLRLAEGKEKQKQIEPIRRLFVGRKIIVGGSTYAASEKIIL
                     EYLHPKPTVQKKPVVAILAPHHVHEARISSIEALCHNFSLTALRLSQVGQSSIRKKID
                     VLIVDSLGVLPHLYPLADVAYVGGGFEGSVHSVIEAALAGVAVVTGPFIQNSAEAMDL
                     QRECLLVAMEKQSCTLFAQIVENLCINKIKNARKAAAFAKERVGASQKIMHTVMDDIS
                     VNSMLA"
     gene            873..1484
                     /locus_tag="PLY93_14480"
     CDS             873..1484
                     /locus_tag="PLY93_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRP70729.1"
                     /translation="MAYAPIVIEQTNRGERHYDIYSRLLKDRIVFMGFPLDDAYANVV
                     IAQLLFLESEDPEKDIYLYINSPGGYVSSGLAIYDTMQLIRPDVRTVCTGQASNIAAL
                     ILAAGTKGKRSALPHSRILLHQPVGGASGQAADIEIQAKEVLRIRDTLNSLYQKHTGL
                     TLEKIEEETARDLYMDGAEAKKWGLIDYVITARDDSASKELAG"
BASE COUNT          416 a          351 c          403 g          376 t
ORIGIN      
        1 acttggtgag cgcaacgcta cctgaggcat cgaagcgata ccaatggccg cttaagaatt
       61 ttttgcaccg tgtgtacgca ccgatgcggc aaatttttgc catcaacgaa gagcacgcgg
      121 agcgcttgcg acgtatttcc cccctcaacg tgagcattgc aggcgatacg cgttttgatg
      181 caattcgatt gcgtcttgcc gaaggtaagg aaaagcaaaa acaaatagag cctatccgcc
      241 gcctttttgt ggggcgtaaa attattgtgg gcggttcgac gtacgcagct tccgagaaaa
      301 taattctcga atatttgcat ccaaagccca cagtgcaaaa aaaaccggtg gtggcaatcc
      361 tcgcgccgca ccacgtccac gaagcacgga tttcgtcgat tgaggcgctt tgccataact
      421 tttctctcac tgcgctccgc ttgagccaag tgggtcaatc ttccataaga aaaaaaatag
      481 acgtgctcat tgtcgatagt ttgggtgtgt tgccgcatct ttaccccctt gccgacgtgg
      541 cgtacgtggg tggaggtttt gaagggtcgg tgcattcggt gattgaggcg gcgctcgcag
      601 gtgtggcggt ggtgacaggg ccctttatcc aaaattcggc agaagcgatg gatttacaac
      661 gcgagtgcct tttggtagct atggaaaaac agagctgtac gctttttgcg caaattgtgg
      721 aaaatttgtg tataaacaag ataaaaaatg cccgaaaggc cgcagcattc gcgaaagaaa
      781 gagtgggcgc gtctcaaaaa attatgcata ctgtgatgga cgacatatcc gtcaattcta
      841 tgctcgcatg agtaaacaat taaggagttc acatggcgta tgcacccatt gtaatcgaac
      901 aaactaaccg aggcgaaaga cactacgata tttattcgcg gcttttaaaa gaccgcattg
      961 tgtttatggg atttccttta gacgacgcgt atgccaacgt ggtaattgca cagttgttat
     1021 ttttagaatc cgaggatccc gaaaaagata tttatcttta tatcaactcc cctggtggct
     1081 atgtttcgtc gggattggcg atttacgaca ccatgcaact catccgcccc gatgtgcgca
     1141 cggtctgcac cggccaagcg tcaaacatcg ctgcgcttat tttagcggcg ggtacaaaag
     1201 gtaagcgttc ggcgttgccg cattcgcgca tcctgttgca ccaacccgtg ggtggggcgt
     1261 cggggcaggc agccgatatt gaaatccaag cgaaagaggt gttgcgcatt cgcgatacat
     1321 tgaatagttt atatcaaaag cataccggtc tcaccctcga aaaaatcgag gaagaaaccg
     1381 cacgcgatct ttatatggat ggcgcagagg caaaaaaatg gggactcatc gactatgtaa
     1441 tcactgcacg cgacgacagc gcatcgaaag aactggccgg ttgagcgatt tttgacgata
     1501 attaaagaga actgaagagg tagtatggca gaaaagcgtt cttatg
//
LOCUS       DAOMPD010000586         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30010_length_1543_cov_2.610215, whole genome shotgun sequence.
ACCESSION   DAOMPD010000586 DAOMPD010000000
VERSION     DAOMPD010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30010_length_1543_cov_2.610215"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1063
                     /locus_tag="PLY93_14485"
     CDS             206..1063
                     /locus_tag="PLY93_14485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP70730.1"
                     /translation="MNFFPPCLNSLFYPVLCPLCGATPKAFSNPFFAVCENDKLVIHT
                     AFKETNYEENYFEKEYVAQYGKSYIDDKPAISARAKWRYERVQHFLKPTTHPRLLEVG
                     SAAGFFLEAMQAQGFQTEGWEVSESMTEYANAHSITTYTHDFFGGAMEHLRQQKPRFD
                     AVALFYVLEHFADQKKAWELLSSLVREGGFLLLSLPSVDGPTFHYAREKWYSTHPQDH
                     AVDYSPASLERIGMRFGFKCKAVFSEGIHPQRFPWGEFPPIAYFYRKMLEKLPISDTI
                     FAILERTHP"
     gene            1060..>1543
                     /locus_tag="PLY93_14490"
     CDS             1060..>1543
                     /locus_tag="PLY93_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70731.1"
                     /translation="MTKKNNTFFDLLTQVSLPAQNQTLLNLEKFWFQDGISNTFAEEL
                     KRETIPLKLSVSKAEIAAVQKCAVQAGLAAQHAHETLEVLLDVCGRQPTKIIFSLKVF
                     EHITPRIHIEENTLSLGNDALLEDALAAATLLARFPQLVYTAPPTIETENALKLPQAF
                     R"
BASE COUNT          421 a          388 c          318 g          416 t
ORIGIN      
        1 atgtttgact tttccacggc tgatggtttt tgtttttttt tgcggcatac aatgtcgggc
       61 aaaataacaa tacaaacatt agaaaaggcc catataattt gcaaacaata tgctttctca
      121 ttttattcct ataccgagcg gcaacataga ataatctata gaaaagtcaa tatttatggg
      181 ctcttcagag acctactctt agtgaatgaa tttctttccc ccttgtctca actctctttt
      241 ttaccctgtg ctttgtcctc tttgtggcgc aactcccaag gcattctcca accctttttt
      301 tgccgtatgc gaaaacgata agcttgtgat tcataccgct tttaaggaaa caaactacga
      361 agagaattat tttgaaaaag agtacgtagc ccagtatggg aagtcgtaca tcgacgacaa
      421 accggcgatt tctgcacggg ctaaatggcg gtacgaacgc gttcaacatt ttttgaagcc
      481 gacaactcat ccccgtcttt tggaagtggg gtcggctgcc ggtttctttc ttgaagcgat
      541 gcaagcacag ggttttcaaa cagagggatg ggaagtttca gagagtatga cagagtacgc
      601 caacgcgcac tcaattacca cttacacgca cgattttttt ggcggcgcaa tggaacatct
      661 aagacaacaa aagcctcgct tcgatgccgt cgctcttttt tatgtactcg aacactttgc
      721 cgatcaaaaa aaagcatggg agcttttgtc ctcgctcgta cgcgaaggag gatttcttct
      781 tctatcgctc ccctctgttg atggacccac attccactac gcgcgtgaaa aatggtatag
      841 cacccacccc caagaccacg cggtggatta ttcccccgca tcgcttgagc gtattgggat
      901 gcgttttggt tttaagtgca aggccgtttt ttcggagggg atacatcccc aaagatttcc
      961 atggggagag tttcctccta tagcgtattt ttaccgtaaa atgcttgaaa aattaccgat
     1021 cagcgacact atctttgcca ttcttgagcg gacgcaccca tgacaaaaaa aaacaatacc
     1081 ttttttgatt tacttacgca ggtctcactt ccggcgcaaa accagactct ccttaactta
     1141 gaaaagtttt ggttccaaga cggcatctct aacacgtttg ccgaagagtt gaaacgggag
     1201 acaatccccc tcaagctttc cgtatcgaaa gcggaaatag ccgccgtcca aaaatgcgcg
     1261 gtacaggcag ggcttgcggc acaacacgcg catgaaactt tagaggtttt actcgatgtg
     1321 tgtggacgac aacccacaaa aattatcttt tcgcttaaag tctttgaaca cataacgcct
     1381 cgaatccaca tcgaagaaaa tacgctttca ctcggcaacg acgcactttt agaagatgct
     1441 ctcgccgcag ctacgctatt ggcgcgcttc ccgcaactgg tatacaccgc accaccgact
     1501 atcgaaaccg aaaatgcact caagctaccg caagcattcc gcc
//
LOCUS       DAOMPD010000587         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30017_length_1543_cov_2.243952, whole genome shotgun sequence.
ACCESSION   DAOMPD010000587 DAOMPD010000000
VERSION     DAOMPD010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30017_length_1543_cov_2.243952"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLY93_14495"
     CDS             <1..330
                     /locus_tag="PLY93_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70732.1"
                     /translation="TGVAHILSLVASSAKPLSAWVETLPQYFMVKRVYRNVTPDALTK
                     AASSAARFIPNAEMDKRDGIHVSGNIDGRPLWVHLRSSNTEPVVRLIAEASSPEDLKR
                     ILDAVKL"
     gene            327..827
                     /locus_tag="PLY93_14500"
     CDS             327..827
                     /locus_tag="PLY93_14500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRP70733.1"
                     /translation="MKVLYRDKEIAFTLVKTRFGEKPLVEYPAAAGILAVEPGRLKKK
                     FLWLIEQYRLGSGAKSWEIPAGKKKKQETILQCAKRELAEEAGLKARFWKKGIHYHPA
                     IGISTEILHLFIARQLSPQEIPHDEDEIFSNKKAFSLAELKRMIKSHKITDGKTLLAY
                     CLFEKI"
     gene            870..>1543
                     /locus_tag="PLY93_14505"
     CDS             870..>1543
                     /locus_tag="PLY93_14505"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRP70734.1"
                     /translation="MRISVIGAGSFGTALAKLVCENGHDTLLWSHSQDTATAVNSKHE
                     NSIYLKEIPLPTALRATVDFAEASEYAELIISATPTQAIREVYTQDKRFKNINVPVVV
                     ASKGIEKKSHMLVCDVFVDIFGESKKKNLFFLSGPSFAREIGLKMPTAITIAGYDAVE
                     LPEIQKVFHNEYFRAYATGDVIGVELGGALKNVMAIATGIADGLKLGSNTRAAIISRG
                     LAEIAR"
BASE COUNT          407 a          354 c          409 g          373 t
ORIGIN      
        1 accggtgttg cgcatattct ttccttggtc gcgtcgagcg caaagccgct ttcggcgtgg
       61 gtggagacat tgccccagta ttttatggtg aagcgagtgt accgcaatgt aacgcccgat
      121 gcgttaacaa aagcggcaag tagcgctgca cgcttcatcc ccaacgcaga gatggataaa
      181 cgcgacggca tacacgtttc tggtaatata gatggtcgcc cattgtgggt gcacttgcgc
      241 tcctcgaata ccgagcccgt ggtgcgcctc atcgccgagg catcttctcc agaagactta
      301 aagcggatac tggatgcagt aaagttatga aggttcttta tcgcgacaaa gaaattgctt
      361 ttacgttggt gaagacgcgc tttggcgaaa aaccgttggt ggagtatcca gcggcggcgg
      421 gaattttagc ggtggagcct ggtcgtctca agaaaaaatt tttatggctc atcgagcagt
      481 accgcttggg ctcgggtgca aagtcatggg agatacctgc ggggaaaaag aaaaaacaag
      541 aaaccatttt acagtgcgct aagcgcgaac tcgccgagga ggcgggactt aaagcgcgct
      601 tctggaaaaa gggcatccac taccaccctg ccatcggcat ttctacagaa attttgcatc
      661 tatttattgc acgccagtta tccccccaag aaatccccca cgacgaggac gagatttttt
      721 ctaataaaaa agcttttagt ttggcagagc tgaagcgaat gataaaaagt cataaaatca
      781 ccgacggtaa gacacttttg gcgtattgcc tttttgaaaa aatataaaga atgagctgac
      841 agcgcgttgc ggcgcggata ggttgcatta tgcgtatatc ggtgattggt gcggggtctt
      901 ttggtactgc gcttgctaaa cttgtttgtg aaaacgggca cgacacttta ctctggagcc
      961 actcgcaaga cacggcgacg gcggtgaata gcaaacacga aaattctatt tacctgaaag
     1021 aaatccctct acctacagcg ttacgcgcga ccgtcgattt tgccgaggct tcagagtacg
     1081 ccgaactcat cattagcgca acccccacgc aggccatccg cgaagtgtat actcaagata
     1141 agcgtttcaa aaacatcaac gtgccggtgg tggtggcctc aaaaggcatt gaaaaaaaat
     1201 cgcacatgtt ggtgtgcgat gtttttgttg atatttttgg tgaaagtaaa aagaaaaatc
     1261 ttttcttttt aagcggtccg tcgtttgcgc gggaaattgg gctcaagatg cccaccgcca
     1321 tcacgattgc cggttacgac gcggtagaac tacccgaaat tcaaaaagtt tttcacaacg
     1381 aatacttccg tgcgtatgcc accggggatg tcattggagt ggagttggga ggcgcgctca
     1441 aaaacgtgat ggcgattgcc accggtattg ccgatggctt aaagctgggg agtaataccc
     1501 gtgcggcgat tatcagccga ggccttgctg aaattgcgcg ctt
//
LOCUS       DAOMPD010000588         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30095_length_1540_cov_2.075421, whole genome shotgun sequence.
ACCESSION   DAOMPD010000588 DAOMPD010000000
VERSION     DAOMPD010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30095_length_1540_cov_2.075421"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLY93_14510"
     CDS             complement(<1..501)
                     /locus_tag="PLY93_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRP70735.1"
                     /translation="MRNYIKEEIVKQNIKPGLAVILVGDDVASQVYVNGKEKAALELG
                     FTSRVMQLPSTTTQEEVVSHIHAFNNDGQIHGILVQLPLPKHLNENQILQTIRADKDA
                     DGFHYLNQGKLLAGESTTLACTPAGIILMLQSLNLNLSGKHAVVVGRSNIVGKPMAQL
                     LLSVMNM"
     gene            complement(630..>1540)
                     /locus_tag="PLY93_14515"
     CDS             complement(630..>1540)
                     /locus_tag="PLY93_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70736.1"
                     /translation="KKSTHFERANTCRAYAVDFAVKLGSLDNNEPFKDALENELSLHF
                     PETILLFLTREDFFLRRHTAIGLTGEILAHLEVNLVDSVIEKVKQGATTLTPQDFEAS
                     EPLAFLAKYRQVIAHKILHRQEFIALAFIIESDNEKVGALKQMMHTYALQNHISRLRD
                     HATSVLEHADNPIMAIRNFIAACEQNLAKAQEPYAIIVTNIANYTRLHNLYGDALAAE
                     IRDFTRKTLREIMEAQDFATEAYHGHFVSILRQKEAGDAWRLSRVLQKQAGKAYADED
                     RRPLFQHKIYARPHLSVIPFEMLFKS"
BASE COUNT          370 a          336 c          390 g          444 t
ORIGIN      
        1 catgttcatc accgaaagta aaagctgcgc catgggtttt cccacaatgt tggaacgacc
       61 caccaccacg gcgtgtttgc cggagagatt taagtttaac gattgcagca ttaaaataat
      121 ccccgctggt gtacaggcaa gtgtcgtgct ttcgcccgct aaaagtttac cttggtttag
      181 ataatgaaaa ccgtccgcgt ctttgtcggc gcggatggtt tgcaaaattt gattttcgtt
      241 taagtgctta ggtaatggga gctgcactaa aatgccgtgt atctgaccgt cgttgttgaa
      301 tgcatggatg tgcgacacca cttcttcttg cgtggtggtg gaaggtagct gcatcacgcg
      361 ggatgtaaat cccagttcaa gcgctgcttt ctctttgccg ttgacataca cttgcgaagc
      421 gacgtcgtcg cccaccagaa taacggcaag gcctggcttt atgttctgtt ttacaatttc
      481 ttcttttatg taattacgca agcttttttc ccaagagttt tttgccctca acccattcgt
      541 ggggagaaaa tatcgtttgc ggcagtgaat cgatgagggg cataccggtt aatgccaaag
      601 gaagaaacag tctttcaagt acttagcgtt taggacttaa acaacatttc aaaaggaatg
      661 accgataaat gagggcgggc ataaatttta tgctggaata aaggacgtct gtcttcgtcg
      721 gcgtacgctt tgcccgcttg cttttgcagc acgcgcgaga gccgccacgc atcgcccgct
      781 tctttttggc gcaaaatact cacaaagtgc ccatggtaag cttcggtcgc aaaatcttgc
      841 gcttccataa tttcacgcaa tgtcttgcgc gtaaaatctc taatttcagc agcgagagcg
      901 tcgccgtata aattatgtag tcttgtgtag ttggcgatat tggtaacgat aatggcgtac
      961 ggctcttgcg ctttagctaa gttttgttcg cacgcggcga taaaattgcg tatcgccatt
     1021 atagggttgt cggcatgttc gagtaccgat gtggcatggt cgcgcaagcg ggagatatgg
     1081 ttttgtagcg cgtacgtgtg catcatttgt ttcaacgcgc ctactttttc attgtcgctc
     1141 tcgatgataa aagccaacgc aataaattct tggcggtgta gaattttgtg cgcgatcacc
     1201 tgacggtatt tagctaaaaa cgccaatggc tcggacgcct caaaatcttg tggagtgagc
     1261 gtggttgctc cttgctttac tttttcaata acgctatcga ctaaattaac ctcaagatgc
     1321 gctaatattt cgccggttaa accaatggca gtgtggcggc ggagaaaaaa atcctcgcgc
     1381 gttagaaaaa gaagtatcgt ctcggggaaa tggagggaga gttcgttttc aagcgcgtcc
     1441 ttgaatggct cgttgttgtc gagactaccg agttttacgg caaaatcgac cgcataagcg
     1501 cggcaggtat tggcgcgttc gaagtgggtc gatttttttt
//
LOCUS       DAOMPD010000589         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30262_length_1533_cov_2.520974, whole genome shotgun sequence.
ACCESSION   DAOMPD010000589 DAOMPD010000000
VERSION     DAOMPD010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30262_length_1533_cov_2.520974"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1533
                     /locus_tag="PLY93_14520"
     CDS             109..>1533
                     /locus_tag="PLY93_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70737.1"
                     /translation="MQYSYDSRGRLISVAAPQGTTEITYMGAASLQRSIRDPHAGKTE
                     YEYYLTPGEASFGKLKTEIRGGYKTTFEYNAGFGRLSKKTVETENGASLHETTIYAYD
                     ETDTPFGNGKLTTLTYTSTPLGAGSQDGFTIQERYAYDARGEVVQTIRRISHETETLC
                     ADADAMPCLQIFGKQKDNIGRVTEMLYPDGTKTSVEYVDDYFSHVKTIQHPSTSGISV
                     TYSDYTYDVAPHVGRVTYGNGVEHRYIYDQTTGLLNTMKIGTQGTQNFASLLDLQYEY
                     DNSYNIKRITDNVVPDLSAVFDYDANKRIRSAALDSGVVRTYQFDHGSGAGSMGNIVQ
                     KDNRRLTYAAGKTVPASDELFDDTTQQWQPNETFTWSEDGNLLTKGPFIYTYDAGSMM
                     SSAVEDLGGGQVATTRFYYDHTGQRFLKTYTRNAVTIKTWYLGDGIELREKYNPSTGS
                     GQAQFEAYQATKYIYGIDDKKVASI"
BASE COUNT          429 a          374 c          393 g          337 t
ORIGIN      
        1 tgcactattg caaccgccat tgacggcaag caacagagag cctgcgaaaa tatttatgcc
       61 aagactgccc taatcacaga aagctttggc acacccaccg agacatcaat gcagtacagc
      121 tatgattcgc gtggtcgctt gatttcagtc gccgccccgc aaggcacgac tgaaattacc
      181 tacatgggcg cagcgtcttt gcaacgttcg attcgcgatc cgcatgcggg aaaaaccgag
      241 tacgagtatt atctgacacc aggtgaggcg agtttcggga aattaaaaac cgaaatacgc
      301 ggcggttaca aaacaacgtt tgaatacaat gccggttttg gtaggctgtc gaaaaagacc
      361 gtagagacag agaacggtgc gtctctacat gaaacgacaa tctacgccta cgacgagacc
      421 gatacgccgt tcggcaacgg taagctgacg acgctgacat acacctcgac tccgctcggt
      481 gcaggctccc aggatggatt tacgattcaa gaacgctacg catacgatgc gcgcggtgaa
      541 gtggtgcaaa cgatacggcg gatttctcac gaaacagaaa cgttgtgcgc cgatgcggat
      601 gccatgccct gtttacaaat ttttggtaag caaaaagaca atatcggtag agtcacagag
      661 atgctctacc ctgacggaac aaagacgagc gtcgaatatg tagacgatta tttttcacat
      721 gtgaagacaa tccaacaccc ctcgacttct ggcatcagtg ttacctatag cgactacacc
      781 tacgacgttg cgccccatgt tggacgcgtg acttacggca atggcgttga gcatcgttac
      841 atatacgacc agacgactgg gcttttgaat actatgaaaa tcggaacgca agggacgcaa
      901 aattttgcgt ccctactgga tctgcaatac gaatacgata acagttacaa tatcaaacgc
      961 atcaccgaca atgttgtgcc tgatttatcg gcggtgttcg attacgacgc gaataagcgc
     1021 attcgctcgg cggcgctcga ttcgggtgtg gtgcgcacgt accagttcga tcacggcagc
     1081 ggagcgggca gcatgggtaa tattgttcag aaagataacc gccgcctcac ctatgctgcg
     1141 ggtaaaaccg tgcctgcaag cgacgagctt tttgacgaca ccacgcagca atggcagccg
     1201 aacgagactt ttacttggtc ggaagatgga aatcttttga cgaaggggcc gtttatttac
     1261 acttacgatg caggtagtat gatgtcttct gccgtagaag atttgggcgg tggacaggtc
     1321 gcgacaacac ggttttatta cgaccacacc ggtcagcggt ttttgaaaac ttatactcgc
     1381 aacgccgtca cgattaagac atggtatttg ggcgacggga ttgagttacg ggaaaaatat
     1441 aacccttcga caggctcagg gcaggctcaa tttgaagcgt accaagccac aaaatacatc
     1501 tatggcatcg acgacaagaa agtggcgtcg att
//
LOCUS       DAOMPD010000590         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30372_length_1529_cov_1.615332, whole genome shotgun sequence.
ACCESSION   DAOMPD010000590 DAOMPD010000000
VERSION     DAOMPD010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30372_length_1529_cov_1.615332"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLY93_14525"
     CDS             complement(<1..700)
                     /locus_tag="PLY93_14525"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70738.1"
                     /translation="MDIQKAIEKIAGLARASQCSMFDIVAYDTFSESVEVFEKKITNT
                     EIQESKALGIRVFKNSCPGIAFTEKLTEDALKTCLADALSHTAITSPISFQLPKPTAL
                     IAKDFNRLSPDFNQYSFSDLIHFATKLEDNVRSADARIENVPYTGAGRSSVTVHFLNS
                     EHVRHVHTDQDFSAYSAAVLSFEAQKKMGFYSNSRLSFSDLKNLPIAALTVERTLQQL
                     GAKPVKAGKYRVLFS"
     gene            complement(700..>1529)
                     /locus_tag="PLY93_14530"
     CDS             complement(700..>1529)
                     /locus_tag="PLY93_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRP70739.1"
                     /translation="VASESPGALSGFEFFENPDIEKYTRVAAERAVRMLSAGYIEGKK
                     MPIVLGNGFGGVIFHEACGHPLESEALPKKATPFMGKHGEKIAHEKLTAIDDGTIPGS
                     WGSLAVDDEGTPTERTVLIENGILKNYIADKVGALECGVRRTGSARRESYKYAPVARM
                     RNTFIAAGSDSFDAMISSIDDGLYAPKLGGGSVNPATGEFNFAVEEGYRIQHGKIESA
                     VRGATLIGKGHEILPLISMVGSDLELTAGMCGATSGSVPVTVGQPTLKVDSMLVGGR"
BASE COUNT          352 a          405 c          368 g          404 t
ORIGIN      
        1 tggaaaaaag cacgcggtat ttacccgctt tgaccggctt tgcacccaat tgttggagag
       61 tgcgctctac tgttaaagcg gcgataggta aattttttaa atcggaaaaa gaaagccggc
      121 tattgctata aaaccccatt ttcttttgcg cttcaaaaga cagaacggcg gcgctatacg
      181 cgctaaaatc ttgatcggta tgtacatggc ggacgtgctc tgaatttaaa aaatgtaccg
      241 tcacgctcga acgccctgcg cccgtgtaag gcacgttctc aatacgcgcg tctgcgctac
      301 gcacgttgtc ttcgagtttt gtcgcaaaat gaatcaaatc gctaaaactg tattggttga
      361 aatcgggtga aagtcgatta aaatcttttg ctataagcgc agtgggtttg gggagttgaa
      421 acgaaatcgg cgatgtaatc gctgtgtgcg agagtgcatc agctaaacat gtcttgagcg
      481 cgtcttctgt caatttttcg gtgaaggcaa tccccgggca tgagttctta aaaacgcgga
      541 tgcccagcgc tttcgattct tggatttcgg tgttagtaat ttttttctca aagacttcga
      601 cgctttcgct aaacgtgtcg taggcaacaa tatcaaacat gctgcactgt gaagcacgcg
      661 ctaaaccggc aattttctct atcgcctttt ggatatccat tatcttcccc ccacgagcat
      721 actatcgact tttagcgtgg gttgtcccac tgttacgggt acactccccg acgtagcgcc
      781 gcacatgcct gcggtgagtt caagatcgct tcctaccatc gagatgagcg gcaggatttc
      841 atgtcctttc ccgatgagcg ttgcgccacg cacggcgctt tcaattttgc cgtgttggat
      901 acggtagcct tcttcgacgg caaaattaaa ttctcccgtc gcggggttta ccgaaccacc
      961 gccgagtttg ggcgcataaa ggccgtcgtc gatgctggaa atcatagcgt caaaactgtc
     1021 gcttcccgca gcgatgaacg tgttgcgcat ccgcgcaacc ggagcatact tgtacgactc
     1081 gcgccgcgcc gaccctgtgc gacgcacacc gcattcgagc gcccccactt tgtcggcgat
     1141 atagtttttc aaaatgccgt tttcaattaa tacggttctc tcagtgggag ttccttcgtc
     1201 gtccaccgcc aacgaacccc aactgcctgg gatggtgccg tcgtcgattg cggtgagttt
     1261 ttcgtgcgca attttttcac catgcttgcc catgaacggg gttgcttttt tcggcagcgc
     1321 ttcgctctcc aaaggatgcc cgcacgcctc gtggaagatc acgccaccaa aaccattccc
     1381 caacacaatc ggcatttttt tcccttcgat gtaaccggca gaaagcatac gcactgcacg
     1441 ctctgcggca acgcgtgtgt atttttcaat gtcagggttt tcaaaaaatt caaatccaga
     1501 aagcgcgccc ggagattcgc tcgcgacaa
//
LOCUS       DAOMPD010000591         1521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30550_length_1521_cov_2.723738, whole genome shotgun sequence.
ACCESSION   DAOMPD010000591 DAOMPD010000000
VERSION     DAOMPD010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30550_length_1521_cov_2.723738"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="PLY93_14535"
     CDS             complement(<1..1429)
                     /locus_tag="PLY93_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRP70740.1"
                     /translation="MTTIRFRFLLILVLVLVNFFWDTANAVDIQKQASLSGINTDAQE
                     FAPTFSADGKTMIFSSTRGGAYSHLYVAHLIDGKWSSPTPLHSVNSQFNDETPFLSHD
                     AKILLFASDRDGSVEIPRDYNGQIRVSFDIYLAKWDGKTWSNPKPIHEINTQWNEKSP
                     SISRDGRYLFFSTWPFGEMKRSRIRSLDLHANGNVIEDLPPQINSGNQETAFVPGEDN
                     TKYYFSSRRPGGKGGWDIYQTRFENGVWSNPEPVPGNVNTEGNEAFLAFSNGNYYISS
                     TFNTQKHDYDLNIEPAPPTQKTVEPKEEIQIAKEETQKISTLPLEWKVQLIDAQTKEK
                     IPGFVQLSILNPNTENTASYTIKQNGGKEHPILLAAPKKINPKEVVRLTGKSSGYLPT
                     TEDAEIKQLNDGTYLLALKKVKKNASVSIRSIYFDYDSARIKKISEPALQMVLEFLQQ
                     TPKAEFEIIGHTDLNGSEDYNQELSE"
BASE COUNT          348 a          295 c          395 g          483 t
ORIGIN      
        1 tttcagaaag ttcttggtta tagtcctcgc tgccgtttaa gtcggtgtgg ccgataattt
       61 caaattcggc ttttggagtt tgttgtaaaa actcaagcac catttgtaga gcgggttctg
      121 aaattttctt aatacgcgcc gaatcgtaat caaagtaaat cgagcggatg gagacactgg
      181 cgtttttctt aacctttttg agagcgagta agtacgtacc atcgttgagt tgtttgattt
      241 cggcgtcttc ggttgtaggt aagtaaccgc tggatttacc cgtgagacga accacttctt
      301 tagggtttat ttttttcggc gccgcaagga gtatcgggtg ttctttaccg ccgttttgtt
      361 taatggtata gctggctgta ttttctgtgt tgggattaag gatgcttaat tggacaaatc
      421 ctggaatttt ttctttggtt tgtgcgtcga tgagttgtac tttccattcg agagggagcg
      481 tggatatttt ttgcgtttct tcttttgcaa tttggatttc ttcttttggt tcgacagttt
      541 tctgtgtcgg tggcgcaggc tcgatgttca aatcgtaatc gtgtttttgg gtgttaaacg
      601 tgctggagat ataataattc ccattagaga atgcaaggaa agcttcgttg ccttcggtgt
      661 tcacattccc cggcacaggt tcgggattgc tccacacacc gttttcaaaa cgggtctggt
      721 aaatatccca cccgcctttg ccgcccggac ggcgcgatga gaaataatac ttggtgttgt
      781 cttcgcctgg aacaaatgcc gtctcttggt tgcccgaatt gatttgcggc ggtaaatctt
      841 cgatgacgtt accgttggcg tgcaagtcga ggctacgaat acgcgagcgt ttcatttcac
      901 caaaaggcca cgttgagaaa aaaagatagc gaccatcgcg gctaatcgag ggtgatttct
      961 cgttccattg ggtgttgatt tcatggatgg gtttgggatt cgaccaggtt ttgccgtccc
     1021 acttggctaa ataaatatcg aaactcaccc gaatttgtcc attgtaatcg cgggggattt
     1081 caacgctgcc gtcgcggtct gaggcgaaga gtaaaatttt ggcgtcgtgc gaaagaaacg
     1141 gagtttcgtc gttgaattgt gaattcactg aatggagtgg ggtggggctt gaccatttgc
     1201 cgtcgatgag gtgtgcaacg taaaggtgtg aataggcgcc gccgcgcgtt gaagagaaaa
     1261 tcatcgtttt accatcggcg gaaaatgtcg gtgcaaattc ttgcgcgtcg gtgttgatac
     1321 cactgagtga tgcctgcttt tggatgtcta ctgcgtttgc agtgtcccag aaaaaattaa
     1381 caaggacgag aacgagaatc aacaaaaaac gaaaacggat cgtggtcata atttgcctca
     1441 tagcttattc tcggtttttt gtaaaattgc tttagccttt tcctttacgt tttgatgaac
     1501 actttgagaa cataatatgc c
//
LOCUS       DAOMPD010000592         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30608_length_1519_cov_2.339481, whole genome shotgun sequence.
ACCESSION   DAOMPD010000592 DAOMPD010000000
VERSION     DAOMPD010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30608_length_1519_cov_2.339481"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLY93_14540"
     CDS             complement(<1..448)
                     /locus_tag="PLY93_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRP70741.1"
                     /translation="MFTGLIEEVGEITQIIPEGKGTRFTIYCKEILNDMRRGDSIAVS
                     GACQTVEAFDTNYFQIFSIPETLLVTNFKNFRQGSFVNLERALRLGDRLGGHLVQGHV
                     EGVAQVLERNDGDAVNIILKYPSPYIIEKGSVCVDGMSMGRNLYANE"
     gene            complement(464..534)
                     /locus_tag="PLY93_14545"
     tRNA            complement(464..534)
                     /locus_tag="PLY93_14545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(500..502),aa:Gly,seq:tcc)
     gene            complement(629..>1519)
                     /locus_tag="PLY93_14550"
     CDS             complement(629..>1519)
                     /locus_tag="PLY93_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70742.1"
                     /translation="YEWFDDAIVAKDEILQQKKTYLNKYFFIVRLIIYFTVWAYMGYA
                     IRKNSNLQDEKKDAAIKLKLTKLSAAYTLIFAYSFLVATIDIVMSLQPHWFTTMFPVY
                     TFANGWFGAIAAIIIILVTVQKNNALKDVSTEHLHDLGKWLLMSVVFWAYIAFSMHML
                     TWYANMPEETYFLEVRLQGNWKYFTVALWMLHFVIPFLILLSRDIKREGKRIVKVAWY
                     SLFVTFLDIVWIVNGSVLGHEGGHTKVDDLPVSLVDLGLFIGVIGLGGIMFFKAFAKV
                     NEAPTGDIDYETSRHFHQTF"
BASE COUNT          450 a          341 c          328 g          400 t
ORIGIN      
        1 tctcatttgc gtatagattc cggcccatcg acatcccgtc gacgcacacc gaaccttttt
       61 cgatgatata cggtgacgga tactttaaga tgatattaac agcgtcgccg tcgttgcgct
      121 ccaagacttg cgcgactcct tcaacgtgac cttgcacaag atggccgccc aaacggtcgc
      181 ctaaacgcaa cgcccgctct aaattcacaa acgagccttg cctaaaattc ttaaaatttg
      241 tgactaacag cgtttcaggg atagaaaaga tttgaaagta gttcgtatca aacgcttcga
      301 cggtttggca tgcaccggaa acagcaatgc tatctcctct acgcatgtcg tttaaaattt
      361 ctttacaata aatggtaaag cgtgtccctt ttccttcggg gataatttgg gtgatttcac
      421 ccacttcttc aatgagtcca gtaaacatcg tcgattaaaa atgagcgggc aaagggactc
      481 gaaccctcac taaaagcttg gaaggctttt gtgctaccgt tacaccatgc ccgcgcgaag
      541 acaaaataaa aaaaagaaac agcgcggcga gttaaagata ataactcgcc gtaacgtttt
      601 caactattgc agttgcctta cagacgcctt agaacgtctg atgaaagtgg cgcgatgttt
      661 cgtaatcgat atcacccgtg ggagcttcat taactttggc aaaggctttg aagaacataa
      721 tgccgcccaa gccgatgacg ccgataaaaa gaccgagatc aactaagcta acgggtaaat
      781 catcgacttt tgtgtgcccg ccttcgtggc ctaaaactga gccgttgaca atccatacaa
      841 tatctaagaa cgtaacaaag agtgaatacc atgcgacttt cacaatccgt ttaccttcgc
      901 gttttatatc gcgtgagagc aaaataagga atggaatgac aaagtgcaac atccacaatg
      961 caactgtaaa atatttccag tttccttgca agcgcacctc gagaaagtag gtctcttctg
     1021 gcatgtttgc ataccaagtg agcatgtgca tactgaatgc aatatacgcc caaaacacaa
     1081 cggacatgag gagccatttt cctaaatcgt gcaggtgttc agtgcttacg tctttgagtg
     1141 cgttattttt ttgaacggtc actaaaataa taataatcgc agcgatagcc ccgaaccagc
     1201 cgtttgcaaa ggtgtacaca gggaacatcg ttgtaaacca gtgtggttgt agcgacatga
     1261 cgatgtcaat cgttgcgacg agaaaactgt aagcgaagat gagggtatag gcagccgaaa
     1321 gctttgtgag tttgagtttt atagcagcgt cttttttctc atcctgcaag ttggagtttt
     1381 tgcggatggc gtaacccatg tatgcccaaa cggtgaagta gataattaaa cgtacaataa
     1441 agaagtattt attcaaatac gttttctttt gctggagaat ttcatctttt gcaacgatgg
     1501 catcgtcaaa ccattcgta
//
LOCUS       DAOMPD010000593         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30684_length_1516_cov_2.278576, whole genome shotgun sequence.
ACCESSION   DAOMPD010000593 DAOMPD010000000
VERSION     DAOMPD010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30684_length_1516_cov_2.278576"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLY93_14555"
     CDS             <1..337
                     /locus_tag="PLY93_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRP70743.1"
                     /translation="VDDLNTPRALAVVHEYMRDANSYIDEQKEIIAERDAQVIRDTLA
                     YFDRVLGLIDLMPQSGVDADLQKKLDERNAARVAKDFKRADALRDELLKAGYKILDTK
                     TGSHLEKIG"
     gene            372..1103
                     /locus_tag="PLY93_14560"
     CDS             372..1103
                     /locus_tag="PLY93_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRP70744.1"
                     /translation="MILFGFHSVLDTLEKTGFLDGDKLIIARQGADQRLQRVLSKAER
                     ARVHIIRKNYGDLNAIAGSKNHQGIILERSAELPLKTFTKQDFVAAQGGIYLALDGIE
                     DPQNLGAIVRSAVGLNAAGIFLPQKHSAPTGATAMKASAGTLIEMPFCFTGSIAQLMQ
                     FIHDKNAEIPMIALDKTGELFSEADLDFCKGENPTLLVIGAESGISRLALERATHRRR
                     LRMSEALESYNVSVATALALYSLTT"
     gene            complement(1262..>1516)
                     /locus_tag="PLY93_14565"
     CDS             complement(1262..>1516)
                     /locus_tag="PLY93_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRP70745.1"
                     /translation="NPEQAEILIEGAEYTRCEIEEAAQREMVTKLEDFLRRRSKIALV
                     MRKEEIRTAKGIHETCKILFGARSKKAFDEYFVKSASKKR"
BASE COUNT          389 a          375 c          371 g          381 t
ORIGIN      
        1 ggtagacgac ctcaacacac cgcgcgcgtt ggcggttgta cacgagtata tgcgcgacgc
       61 taatagttac atcgacgaac aaaaagaaat tatcgccgaa agagatgcgc aagtgatccg
      121 cgacacgctt gcctacttcg accgcgttct tggacttatc gacctcatgc cgcaaagcgg
      181 tgttgatgcc gatttgcaaa aaaagctgga tgaacgtaac gccgcgcgcg ttgctaaaga
      241 ttttaagcgt gccgatgcgc tgcgcgacga acttttgaaa gcgggctata aaattttaga
      301 tacgaagacc ggttcgcatt tagaaaagat tggctaagaa tgtatcttaa accgacaaaa
      361 ggtggggctt tgtgattctt ttcggttttc actcggttct cgacacgctc gaaaaaacag
      421 gctttctcga cggcgacaaa ctcatcatcg cgcggcaagg cgcagaccaa aggcttcaaa
      481 gagtactctc gaaagccgag cgcgcgcgcg tgcacattat ccgcaaaaac tacggcgatc
      541 ttaacgctat cgccggcagt aaaaaccatc aaggcattat cttagaacgc agcgccgaac
      601 tgccgctgaa aacctttacc aaacaagact ttgttgcagc gcagggcggc atctatctcg
      661 cgctcgacgg tatcgaagac ccgcaaaact tgggcgcgat cgtgcgcagc gccgttggcc
      721 ttaacgccgc cggaattttt ctgccacaaa agcacagtgc gccgacgggc gctactgcta
      781 tgaaagcatc tgcgggtacg ctcattgaaa tgccgttttg ctttaccggc agtatcgctc
      841 agctcatgca gtttatccac gataaaaacg ccgagattcc aatgattgcc ctcgataaaa
      901 cgggggagtt attttctgaa gcggatcttg atttttgtaa aggggaaaat cctactttgt
      961 tggtgattgg tgctgaaagt gggattagcc gcctagcgtt agagcgcgct acacaccgcc
     1021 gccgtttgcg catgagcgaa gctctcgaaa gctacaacgt ttcagtggca acggcactcg
     1081 cgctctattc tttgacgact tgaaaaacac gtattgtgtc ccacgatttc tgattatgtc
     1141 acattgggga taactatctt cgttggatta tgtcgcttta aggggtaact atcctcccaa
     1201 agtgctggaa tcgtcaaaaa tgaggataaa atttgaggaa atactctctt ttagacgatg
     1261 gttaccgctt tttcgacgcc gactttacaa aatactcgtc aaacgctttt ttcgatcgag
     1321 cgccaaaaag aattttgcac gtttcgtgaa taccttttgc cgtgcggatt tcctctttac
     1381 gcatcacgag tgcaatcttg gagcggcggc gcaaaaaatc ttcgagtttg gtaaccattt
     1441 cgcgttgtgc ggcctcttcg atttcacagc gcgtgtactc agcaccttcg atcaaaattt
     1501 ccgcttgttc ggggtt
//
LOCUS       DAOMPD010000594         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30688_length_1516_cov_2.055441, whole genome shotgun sequence.
ACCESSION   DAOMPD010000594 DAOMPD010000000
VERSION     DAOMPD010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30688_length_1516_cov_2.055441"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PLY93_14570"
     CDS             complement(<1..806)
                     /locus_tag="PLY93_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRP70746.1"
                     /translation="MSRKVELKQLRNIGIMAHIDAGKTTTTERILFYTGRSHKIGEVH
                     EGAATMDWMEQERERGITITSAATTCFWKDAAGEKIQINVIDTPGHVDFTVEVERSLR
                     VLDGAVTAYDGVAGVEPQTETVWRQADRYHVPRLCYVNKIDRMGADFYRAVDMMRDRL
                     GANAVPIQLPIGVESSFVGVIDLVTMEAVVWSGEELGAKYERKAIPDDMKEKAQQYHE
                     ALVSAVAEVNDTLTEKYLESGTLSKEDLIAGLREGTIAMKIFPVLCGSSF"
     gene            complement(866..1339)
                     /gene="rpsG"
                     /locus_tag="PLY93_14575"
     CDS             complement(866..1339)
                     /gene="rpsG"
                     /locus_tag="PLY93_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRP70747.1"
                     /translation="MARRRTKSLTRNIGGDAVHGSVLVQKFINAMMYDGKISTAAQLV
                     YDALTRVEEKTGVSGFEQFMKAIENIKPQVEVKSRRVGGVTYQVPIEVYPARKQSLAI
                     RWLVNAARDRSGKTQSDKLANEIMDAINNNGGAIKKKEEVKRMAEANRAFSHYAW"
     gene            complement(1391..>1516)
                     /locus_tag="PLY93_14580"
     CDS             complement(1391..>1516)
                     /locus_tag="PLY93_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRP70748.1"
                     /translation="GGRVKDLPGVRYHIVRGSLDTLGVDKRRKSRSKYGTKRPKA"
BASE COUNT          342 a          407 c          335 g          432 t
ORIGIN      
        1 ttaaatgatg agccgcagag tacgggaaaa attttcatgg caatggtgcc ttcgcgtaag
       61 cctgcgatta aatcttcttt agatagagta cccgactcca aatacttttc agtcagggta
      121 tcgtttacct cagccaccgc tgaaaccaac gcttcgtggt actgttgcgc tttttctttc
      181 atgtcgtcgg gaatcgcttt gcgctcgtat ttggcgccta actcttcacc cgaccaaacg
      241 accgcttcca tggtaactaa gtcgataaca cccacgaatg aagattccac accaatcggt
      301 aattgaatcg gcacggcgtt cgcgcctaaa cgatcacgca tcatgtcgac ggcacggtag
      361 aaatccgctc ccatgcggtc gattttattg acgtagcata agcggggtac atggtagcgg
      421 tcggcttggc gccacaccgt ttcggtttgc ggttcaacgc ccgcgacacc gtcgtaagca
      481 gttacggcac cgtccaaaac gcgtaatgaa cgttcgactt caacggtgaa gtcaacgtgg
      541 cctggagtat cgataacgtt aatttggatt ttttcacccg cagcatcttt ccagaaacag
      601 gtggtcgccg ctgaagtgat ggtgataccg cgttcgcgct cttgctccat ccagtccatg
      661 gtcgccgcgc cttcgtgtac ttcgccaatt ttgtgcgagc ggccggtata aaaaagaatg
      721 cgttctgtgg tggtggtttt tcctgcatcg atatgcgcca tgataccgat atttctgagc
      781 tgtttgagtt ctactttccg tgacataatt tttccttaaa atataatttg cctaaaagcc
      841 acttacggcc attaggcaaa tccgtttacc aagcgtagtg agagaaagcg cggttcgctt
      901 cggccatgcg cttcacttct tctttctttt taattgcgcc gccgttgtta ttgatcgcgt
      961 ccataatttc attggcgagt ttatccgatt gggtcttgcc cgagcggtcg cgtgctgcat
     1021 tgacgagcca acgaatcgct aaagactgtt tccgcgcagg gtacacttcg ataggaacct
     1081 ggtacgtcac cccacccaca cgacgcgact tcacttcgac ttgtggttta atgttttcaa
     1141 tcgctttcat gaattgctcg aaacccgaca cgccggtttt ttcttcgacg cgcgttaaag
     1201 cgtcatagac gagttgtgcc gccgttgata ttttaccgtc gtacatcatt gcgttgatga
     1261 acttctgtac aagcacactc ccgtgtaccg catcaccacc aatgtttctt gttaaactct
     1321 tcgttctacg tcttgccata attcctcttt aaactaatcc ctaaactaat ccaggctact
     1381 caatttatcg ttacgccttc ggacgcttcg ttccatattt tgatcgcgac ttgcggcgtt
     1441 tatccacacc gagagtatct aatgaaccac gcacaatgtg ataacgcaca ccaggtaaat
     1501 ctttcaccct gccgcc
//
LOCUS       DAOMPD010000595         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30704_length_1515_cov_3.223973, whole genome shotgun sequence.
ACCESSION   DAOMPD010000595 DAOMPD010000000
VERSION     DAOMPD010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30704_length_1515_cov_3.223973"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1114
                     /locus_tag="PLY93_14585"
     CDS             269..1114
                     /locus_tag="PLY93_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRP70749.1"
                     /translation="MEKPVPVLDENKIHALALHNASQFLSLKNRRILEHAQNFTHFAE
                     IVLGAQYKKFYREAELFLKESDLQLLLSFEDEDYPPLLKESDYAPFFLYLKGERALIK
                     NSKISIVGSRGPSPMGRKAAASVARYFVAKGCTVVSGIARGVDSIAHHTALDAHGQTI
                     AILPNGFNHLYPLENRDLYLRAAEDKNLLFISEYAPTQKAAKHHFVRRNRIIAALSML
                     TVFVEGGIKSGGLITVRHALAEGREVAALTHPLLLNNAGGEKLIADGALDLSALIVES
                     NAQTY"
     gene            1098..1376
                     /locus_tag="PLY93_14590"
     CDS             1098..1376
                     /locus_tag="PLY93_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRP70750.1"
                     /translation="MPKLTEIYIRQMHFEDAKHKLLSEIEHLFFEGVCEVEIIHGIGN
                     YILRKMVIAELSKLDYVHLDENHFNPNPGSLRATLLVPDENLLERYKG"
BASE COUNT          423 a          308 c          351 g          433 t
ORIGIN      
        1 ccccccaatt gcggcgtagc atttgtcagg tatgtccgtg ggttagacaa aagttgtgat
       61 tatgggataa ctatgccgat ttttatcgat tatgtcacat tatgggataa ctatgccgat
      121 ttttattgat tatgtcgcat taggggataa ctatccttac agaaaatttt ggcataaaaa
      181 gtcatctttt tttaaaaaat ttgtactttt tgtcaaaaaa gtgagaaaaa ttgagcattt
      241 tttggtattt aatatataga ggacaaactt ggaaaagcct gtgccggtgt tagacgaaaa
      301 taaaattcat gcattagcgc tccacaatgc gtcgcaattt ttgtcgctga agaatcggcg
      361 tatccttgaa cacgcgcaaa actttacgca ttttgcagaa attgtgctcg gtgcgcagta
      421 taaaaaattt tatcgcgagg ccgagctttt tttgaaagaa agtgacttac agttgttgct
      481 ctcctttgaa gacgaggact atcctccgtt attaaaagaa agcgactacg cgccattttt
      541 tctttactta aagggcgagc gcgcgttgat taaaaattcc aagatttcga ttgtgggcag
      601 ccgcgggccg tcacccatgg gacgcaaggc tgcagcgagc gttgcacgct attttgttgc
      661 gaaagggtgc actgttgtct cggggattgc acgcggtgtc gatagcattg cacaccacac
      721 cgcgttagac gcacacggtc aaacgattgc gatcttgccc aacggattta atcacctcta
      781 tcctttagaa aaccgcgatt tatatttgcg cgcggcagaa gataaaaatt tactttttat
      841 ttcggagtat gcaccaacac aaaaagcggc gaagcatcat tttgtccgcc gcaatcgtat
      901 tattgccgca ctttcaatgc ttaccgtttt tgtagagggg ggaataaaga gtgggggact
      961 catcacggta cgccacgctc tcgccgaggg gagagaagtg gccgcgttga cgcacccttt
     1021 acttttgaac aacgcgggtg gtgaaaaatt gattgccgat ggagcgttgg atttgagtgc
     1081 gttaatcgta gagtcaaatg cccaaactta ctgaaattta tatccgacaa atgcattttg
     1141 aagatgcaaa gcacaaactg ctttcagaaa ttgaacatct tttctttgaa ggcgtgtgcg
     1201 aagtagaaat catccacggc atcggcaact atattttacg caaaatggtc atcgccgaat
     1261 tatccaagct cgattacgtg cacttagatg aaaatcattt taatcctaac ccgggtagct
     1321 taagagcgac attacttgtc ccagacgaaa atcttttaga gaggtacaag ggttaatccg
     1381 acgattgcgc ttgacgcact tggtcgagcg atttgatgat ataatcgact tcgatattca
     1441 cagaagtgcc gacattccac gtaccgatgt tggttttgcg gagcgtctcg ggtataatct
     1501 gtactcgaaa acgct
//
LOCUS       DAOMPD010000596         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30799_length_1511_cov_3.557005, whole genome shotgun sequence.
ACCESSION   DAOMPD010000596 DAOMPD010000000
VERSION     DAOMPD010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30799_length_1511_cov_3.557005"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLY93_14595"
     CDS             complement(<1..538)
                     /locus_tag="PLY93_14595"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HRP70751.1"
                     /translation="MKHIKKKVFWVFFALLAVAIGCGDPIPMEEMGIAKVAIARAETV
                     KASQYSPQNYDAAQKALLAAHTFVEKDSMGDAKEKALEAKKLADAAYDEAAPKLAQDT
                     RGEAEKAIRAAEEANAEQFAAEDLAAAKASLVQGDTYYETKDFLSSYHLFEEAREKGK
                     RAQTTAEAQVEVMHRELNE"
     gene            complement(667..1002)
                     /locus_tag="PLY93_14600"
     CDS             complement(667..1002)
                     /locus_tag="PLY93_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP70752.1"
                     /translation="MEISQREKDGITILDIQGEIDLYNAPEIKDIIQKLIESQKYNVI
                     INLEKVSYIDSSGIGALISSLSNLKKYQGGLKIINVYASVKKVFELTKLTSFFEIYES
                     EAEALAKFK"
     gene            complement(1123..>1511)
                     /locus_tag="PLY93_14605"
     CDS             complement(1123..>1511)
                     /locus_tag="PLY93_14605"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRP70753.1"
                     /translation="NVISMMYGTSMALTIQKYYTPSGVSIHKKGIEPDIKVAALDFNK
                     DDRRHYKEIKEKKILKDFVKEHKHYNADTVKKFQKMLEARGLTLSDFAAKRTLKDELM
                     KDEPKQLIDLEFDVQLKRAIQELSKT"
BASE COUNT          367 a          338 c          266 g          540 t
ORIGIN      
        1 cctcgttgag ttcgcgatgc atcacttcaa cttgtgcctc agcagttgtt tgtgcacgct
       61 ttcctttttc gcgcgcttct tcaaaaagat ggtaagagct taaaaaatct ttggtttcgt
      121 agtaagtgtc gccttgtact aaactcgctt tggcagcggc taaatcttca gcggcaaact
      181 gctcagcgtt tgcttcttct gccgcacgga ttgctttttc cgcttcgcca cgagtgtctt
      241 gtgcgagctt aggcgccgct tcatcgtaag ctgcgtcagc gagtttttta gcctccaggg
      301 ctttctcttt tgcatcgccc atgctgtctt tttcaacaaa cgtgtgcgcg gccaagagag
      361 ctttttgggc agcgtcgtag ttttgcggag aatactgcga ggcctttacc gtctcagcgc
      421 gtgcaattgc gactttagca atccccattt cttccatcgg tataggatca ccgcagccta
      481 tagctacggc aagcaatgca aaaaataccc aaaaaacttt ctttttaatg tgtttcatat
      541 atcctcgtta aactctcttc aaatctttgt cctaaaacct ttttttcaaa agttttcaaa
      601 atttctaact ttcttcaatt acttttatca attcttccat atttccttca aatagaagag
      661 gaatacttac ttaaacttag ccaacgcttc agcttctgat tcataaattt caaagaaact
      721 tgtcaacttc gtgagctcaa acactttctt gacggaagcg tacacattga taatcttcaa
      781 gccgccttgg tacttcttta agttcgacaa actggaaatc aatgcgccga tgccggatga
      841 atcgatatac gaaacttttt ctaagttgat aatgacatta tatttttgcg attcaatgag
      901 tttttgaata atgtctttta tctctggcgc attataaagg tcaatctccc cctgaatgtc
      961 caaaattgtg attccatctt tttctcgttg gctaatttcc atctttctac tccgctggta
     1021 tttttttaac ctataaattt attagtaaat atgctgtcta cctatcctta aggctttctt
     1081 taacgtcaat caaaaaacag attttttgct ccatagtaaa cattatgttt tgctcaactc
     1141 ttgaatcgcc cgttttagtt gaacatcaaa ctctaaatca ataagctgct taggttcatc
     1201 tttcatgagt tcgtctttta gggtgcgttt ggcagcaaaa tcagaaagcg ttaatcctcg
     1261 cgcttcgagc attttttgga atttttttac tgtgtcagcg ttgtaatgtt tatgctcttt
     1321 gacaaaatct ttcaatattt tcttttcttt gatttctttg tagtggcgtc tatcgtcctt
     1381 gttgaaatca agcgcagcga ctttgatatc cggttcaatc ccctttttgt ggatcgacac
     1441 tcccgacggt gtatagtatt tttggatagt caatgccatc gacgtgccat acatcatgct
     1501 aatgacattt t
//
LOCUS       DAOMPD010000597         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_30818_length_1511_cov_1.833104, whole genome shotgun sequence.
ACCESSION   DAOMPD010000597 DAOMPD010000000
VERSION     DAOMPD010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30818_length_1511_cov_1.833104"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLY93_14610"
     CDS             <1..389
                     /locus_tag="PLY93_14610"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRP70754.1"
                     /translation="GATHTTFVELAQERLNHIQRALTHLNISKESYTLVRTRAAKVLQ
                     EAFEETLPCVVWADPPYTYNSTPSNDPANLLTLYREARMAHTHPPLLIIQAHEKNGAL
                     ADEYLAQHNDVKVYRYGSNCLLVLEK"
     gene            386..>1511
                     /locus_tag="PLY93_14615"
     CDS             386..>1511
                     /locus_tag="PLY93_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRP70755.1"
                     /translation="MKTPTKSVTIKNAEVVLPDAVHTVDVHLKDGKIFAIGSHSDFNA
                     EEVVDATGLTLMAGVIDPQVHLRDPGMTHKEDLATGSRAAAAGGVTTFFEMPNTRPAT
                     TTRALMEEKKTIAREKCVVNYNFFIGATKDNFSELDSVPNVPGIKVFMGSSTGDLLVD
                     KHNDIENIFAHTRRRIVVHAEDEEILNAQKQYIVPGKNFAQHPDIRPASAALKATQFA
                     VAMAEKYNHPLHVLHLTTKDEVDFLAPKIGKLITTETSPQHLWFSSPEIYDRLGSFAQ
                     MNPPIRTADHRDALRRGIKSGIIDIIATDHAPHTREEKAQDYPKAPSGMPGLETSLAA
                     ILTLRDTLGISLVQVSQIMAANAARIYGIKNRGSIAVGHYA"
BASE COUNT          417 a          416 c          349 g          329 t
ORIGIN      
        1 taggcgccac gcataccacc tttgtcgaac tcgcgcaaga gcgtctcaac catatccagc
       61 gcgcattaac acatttgaat atctccaaag aaagttatac cctcgtgcgc acccgtgccg
      121 ccaaagtttt gcaagaagcg tttgaagaaa cgcttccatg cgtggtgtgg gcagaccccc
      181 cttacactta caacagcaca ccgtcaaacg acccggcaaa cttactcaca ctctaccgcg
      241 aagcacgtat ggcgcacacc catcctcctt tacttatcat ccaagcgcat gaaaaaaatg
      301 gcgcgctcgc cgacgagtat ttggcgcaac acaacgatgt taaagtgtac cgctacggtt
      361 caaactgttt attggtactt gaaaaatgaa aacacctaca aaaagcgtca cgattaaaaa
      421 tgcagaggtc gtccttccag acgccgtgca tacggtggat gtccatctta aagacggtaa
      481 aatttttgcg ataggaagtc attcggactt taacgccgaa gaggtcgtcg acgcaacggg
      541 gttgacactc atggcaggcg ttatcgatcc acaagtgcat ttacgcgacc cgggtatgac
      601 ccacaaagaa gatttagcta ccggttcgcg cgcggcagca gcgggcggtg tgaccacgtt
      661 ttttgaaatg cccaataccc gccctgccac tacgacgcgc gcgctcatgg aagagaaaaa
      721 aactatcgcc cgcgagaagt gcgtggtgaa ctataatttt tttatcggtg ctactaaaga
      781 taattttagc gaactcgaca gcgtgcccaa cgtaccgggt atcaaagtat ttatgggttc
      841 gtccacgggg gacttactcg tcgacaaaca taacgatatt gaaaatattt ttgcgcacac
      901 ccgccgccgt attgtggtgc acgctgaaga cgaagaaatt ttgaacgcac aaaaacaata
      961 cattgtccct ggaaaaaatt ttgcgcaaca ccccgatatc cgtcccgcat cggcagcgtt
     1021 aaaagcgacg caatttgcag tggcgatggc cgagaaatat aatcaccccc tgcacgtcct
     1081 ccatcttaca accaaagacg aagtggactt tcttgcgccg aaaattggta aactcatcac
     1141 caccgaaacg agtccacagc atttgtggtt ttcctcccca gaaatatacg accgcttggg
     1201 ttcttttgcg caaatgaatc ctcccatacg cactgccgat caccgcgatg cattgcggcg
     1261 cgggatcaaa agcggtatca tcgacatcat cgctaccgac cacgcaccgc acacgcgcga
     1321 agaaaaagca caagactacc ccaaggcgcc ttcgggaatg ccgggcttgg aaacgtcgct
     1381 cgcggcaatt ctcaccttgc gcgacactct tggtatttcg ttggtgcaag tgtctcaaat
     1441 tatggcggca aatgcggcgc gcatttacgg catcaaaaat cgtggcagta ttgcggtggg
     1501 tcactatgcc g
//
LOCUS       DAOMPD010000598         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31090_length_1501_cov_2.120332, whole genome shotgun sequence.
ACCESSION   DAOMPD010000598 DAOMPD010000000
VERSION     DAOMPD010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31090_length_1501_cov_2.120332"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1501
                     /locus_tag="PLY93_14620"
     CDS             19..>1501
                     /locus_tag="PLY93_14620"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRP70756.1"
                     /translation="MRSLATRTENAALSPTLKDIVNHVRSEAFDLVFIESAGVGQSDT
                     SITELVNFSIYVMTPEYGAATQLEKIAMLDFADMVIINKFDRMRAEDALQQVKKQYLR
                     NHAPGDTRSAVAPVYPTIANRFGDKLVDDAFAALIIHLKAKQLLPDSLPSPQKTESIS
                     LVDDFIPAKRSRYLSDIADAVRGYHTDADTQAELAQRAQALFTSMRLLGYSKSRLGEI
                     AEANDAEPLNSLVSKYNETLAKVHPTLLASLAAIETTRTNYAQEKFSYKVRGKDVTVR
                     AGVRSLSNTLTAKVSPAPYKDWGELSTFLLKENIPGEFPFTAGVFPFKREGEDPTRMF
                     AGEGSPERTNRRFHLIAGGPEAKPSSTRDASVARLSTAFDSITLYGENPDERPDIFGK
                     IGNAGVSICTVDDIKKLYSGFDLSAPTTSVSMTINGPSPTILAFFMNAAIDQKIEQYL
                     HATGNLETTRAHIADNYRGKNLPVPVYGDALPETHNGLGLSLLG"
BASE COUNT          373 a          440 c          378 g          310 t
ORIGIN      
        1 aacgacggca aaatctatat gcgctcgctc gcgacgcgca ccgagaacgc agcgctctcg
       61 ccaacgctca aagatattgt aaaccatgtg cgttcagaag cctttgattt agttttcatc
      121 gaatcggcgg gcgtggggca gagcgacacg tcgatcactg aactcgtgaa tttttccatc
      181 tacgtgatga cacccgagta cggcgcagcg acgcagctcg aaaaaatcgc catgctcgat
      241 tttgccgaca tggttattat caacaagttc gaccgcatgc gcgccgaaga cgcattgcag
      301 caagtgaaga agcagtattt gcgtaaccat gcgcccggcg acacccgctc ggcggtagca
      361 cccgtctacc ccaccatcgc aaaccgtttc ggcgataaac tcgtcgacga cgcattcgct
      421 gcgcttatta tacatttgaa ggcgaaacaa ctcttacccg atagtctgcc ttcaccgcaa
      481 aaaaccgagt cgatttcact cgtcgacgat tttattccgg caaaacgatc gcgctatcta
      541 tccgatattg ccgacgcggt gcgtggctac cacacagacg ccgacaccca agccgaactc
      601 gcgcaaagag cacaggcgct tttcacttcg atgcgcctct taggttatag caaaagtcgg
      661 ctcggcgaaa tcgcggaggc aaacgatgct gaaccactca acagcctcgt gtccaagtat
      721 aatgaaaccc tcgccaaggt acatcccact ttgctggcgt cgctggcggc catcgagacg
      781 acacgcacaa actatgcgca agagaaattc tcgtataaag ttcgcggtaa agacgtgacg
      841 gtgcgcgcgg gtgtgcgctc gctgtccaat acgctcaccg caaaagtctc gcccgcgccg
      901 tacaaggact ggggtgaact ttcaaccttt ctactgaaag aaaatatacc gggtgaattt
      961 ccatttaccg ccggcgtctt tccctttaag cgcgaaggcg aagacccgac gcgcatgttc
     1021 gcgggagaag gttcacccga gcgcacgaac cgacgcttcc atttgattgc aggtgggcca
     1081 gaagcgaagc cttcgtcgac acgggatgcc tccgtggcgc gactttcaac cgcgttcgac
     1141 tctatcacgc tctacggcga gaaccccgac gagcgtccgg atattttcgg aaaaatcgga
     1201 aacgcgggcg tgtcaatctg caccgtcgac gacatcaaaa aactctattc ggggttcgat
     1261 ctttcagcgc cgacgacgag cgtttcgatg acgattaacg gcccttcgcc cacgattttg
     1321 gcgttcttta tgaacgcggc gatcgaccag aagattgaac aatacttgca cgctaccggt
     1381 aatcttgaaa cgacaagagc gcacattgcg gataactacc gcggcaaaaa cttgcccgtg
     1441 ccggtctacg gcgacgcgct gcccgaaacc cataatggtc taggactatc tcttttgggc
     1501 g
//
LOCUS       DAOMPD010000599         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31106_length_1500_cov_2.956401, whole genome shotgun sequence.
ACCESSION   DAOMPD010000599 DAOMPD010000000
VERSION     DAOMPD010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31106_length_1500_cov_2.956401"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLY93_14625"
     CDS             <1..313
                     /locus_tag="PLY93_14625"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803974.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRP70757.1"
                     /translation="TTVYHSRTQGLNDFVSSADVLVSATGVRGVVDTACIKPGAVVID
                     VGMHRLDGRLTGDLDLAPAAEKAAYYTPVPGGVGPMTIAMLLFNTYRNARALTNPTLL
                     A"
     gene            complement(324..509)
                     /locus_tag="PLY93_14630"
     CDS             complement(324..509)
                     /locus_tag="PLY93_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70758.1"
                     /translation="MAVLIVLGGTLGGALLGFVIPFSLAQFMPSYGPALSFVPMITVP
                     LGFLLGLIGGIFWAVYR"
     gene            complement(547..1368)
                     /locus_tag="PLY93_14635"
     CDS             complement(547..1368)
                     /locus_tag="PLY93_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70759.1"
                     /translation="MQNPFKRIFKRNENITDSIAPQTAVESASQSAHTVLDKRKNNVQ
                     RKKRIYKSSSPYIFDKALAARVEKQLVDELQKNVAQYRTDKWSLLEIADQILKDKIGI
                     QRPPQLRAHRIFDAFVRIKKSRSTLVKGDCHEWRGTVKNSEPVITTLNSQSNKRIVVD
                     TRKYILENPRQGKRRNFRSKPFNVCGNTLCVNPQHIEMRGKERAEKAGENHPRAKYPD
                     SFFRKIAKEYNAGATAKELGEKYNIGYTYIEAVMRKEKRIDATEGMTIRGRFTTY"
BASE COUNT          334 a          375 c          361 g          430 t
ORIGIN      
        1 gacgaccgtt taccactcgc gcacacaggg gttaaatgat tttgtttcct cggcggatgt
       61 cctggtgagc gcaacgggcg tgcgtggtgt tgtcgacaca gcgtgcatta aaccaggggc
      121 ggttgtcatc gacgtgggga tgcaccgctt ggacggccgc ctcaccggcg acttagattt
      181 agcgccggcc gctgaaaagg cggcgtatta tacgccggtg ccgggcggtg ttggtcccat
      241 gacgattgcc atgctacttt ttaatacgta ccgtaatgcg cgggcgttga caaaccctac
      301 tcttttagct taacctatcg cttttaacga tacaccgccc aaaagattcc accaatcagc
      361 cctagcaaaa agccaagggg tacagtgatc atcggcacaa agctgagtgc aggcccatac
      421 gaaggcataa actgcgcaag agaaaaggga atcacaaacc ccaaaagtgc accgcctagt
      481 gtacccccca aaacaatcaa taccgccata accactcctt actcccgcaa gttgtcgtat
      541 ccgcttttaa tacgttgtga aacgcccacg gatggtcatg ccttctgtgg cgtcaatacg
      601 cttttctttg cgcatcaccg cttcgatata cgtataacca atgttgtatt tttcacccaa
      661 ctcttttgca gttgcgcccg cattgtactc ttttgcaatt ttacgaaaaa acgaatcggg
      721 gtatttcgcg cgtgggtggt tttcacccgc tttttctgcg cgttctttgc cgcgcatttc
      781 aatgtgctgc ggattcacgc atagcgtatt accgcacacg ttaaatggtt tcgagcggaa
      841 attacgccgc ttcccttgac gcggattttc taaaatatac tttcgtgtat cgacgacaat
      901 acgcttgttg gattgcgagt tgagcgtagt gatgaccggt tcgctatttt taaccgtgcc
      961 ccgccattcg tggcaatcgc ctttcactag ggtgctgcgg ctttttttta tgcgcacgaa
     1021 agcgtcaaaa attctgtggg cacgcaactg gggtgggcgc tggatgccga ttttatcttt
     1081 gagaatctga tcggcgattt ctaaaagcga ccatttatcg gtgcggtatt gcgccacatt
     1141 tttttgtaac tcgtcgacca gttgtttttc aacacgcgcc gccaatgctt tatcgaaaat
     1201 ataaggagag ctacttttat agattctttt tttacgctga acgttatttt ttcgcttatc
     1261 gagtactgta tgtgcgcttt gggacgccga ctcgaccgcc gtttgtgggg cgatagagtc
     1321 ggttatattt tcattgcgct taaaaatgcg cttgaaaggg ttttgcattt tttagtacat
     1381 actctcgatc agcttcgcgt acttatcggc aatcacgttt cgcttcattt tgaagaggtt
     1441 ggtgatttca tcgcccactt ccatcggttt gggtaagaag cggaaatccg acacgcgctc
//
LOCUS       DAOMPD010000600         1499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31139_length_1499_cov_1.484072, whole genome shotgun sequence.
ACCESSION   DAOMPD010000600 DAOMPD010000000
VERSION     DAOMPD010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31139_length_1499_cov_1.484072"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>1499
                     /locus_tag="PLY93_14640"
     CDS             130..>1499
                     /locus_tag="PLY93_14640"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450242.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRP70760.1"
                     /translation="MKIKRHFTTSGKDIYSDIIFEKRSSRITNTDGSVAFEANEVEVP
                     SFWSNVATDILAQKYFRKAGVPRALKSVPEEGIPEWLYRQVPDEEQLGKFPKEERYTR
                     ETSSKQVMHRLAGAWTYWGYKHNYFSSEEDARAFYEELSYMLMNQMAAPNSPQWFNTG
                     LHWAYGVDGPSQGHYYVDPTTGKLVKSKSSYEHPQPHACFIQAVDDDLVNAGGIMDLW
                     VREARLFKYGSGTGSNFSALRGENERLSGGGKSSGLMSFLKIGDTAAGAIKSGGTTRR
                     AAKMVCLDIDHPDVEAFIDWKVTEEAKVASLVSGSKAIRRVINEVFSAVAGCVKQPAL
                     LDPKQNHILALALKKARASSVPDSYVQRIIHLARQGFTSIEFEEYDTDWTSKAYLTVA
                     GQNSNNSLRLSNAFLKAVESNGDWELIRRIDGKVHKTLSARDLFDKLSFAAWSCADPG
                     LQFDTT"
BASE COUNT          393 a          385 c          372 g          349 t
ORIGIN      
        1 aagaagaaat ttgtaaaaaa ttgtctcagc gagttgatgc actgcaaaat ttttttttcg
       61 ccgcgagaaa tccaattcga ttgacgcaat gtgacataat ctaatgctca ccctctacga
      121 ggattatcta tgaaaattaa acgacatttt acaacaagcg gcaaagacat ctatagcgac
      181 attatttttg aaaaacgttc atcgcgcatc accaacaccg acggctctgt ggcgtttgaa
      241 gccaacgaag tggaagtccc ctcgttttgg tccaacgtgg cgaccgacat cctggcgcaa
      301 aagtattttc gtaaagcggg cgttccccgt gcattaaaaa gcgttcccga agaaggtatc
      361 cctgaatggc tctaccgcca agtacccgat gaagagcagt tgggtaaatt ccccaaagaa
      421 gaacgttata cccgcgaaac ctcttctaaa caagtgatgc accgcctcgc cggtgcatgg
      481 acgtattggg ggtataagca taactatttt tccagcgaag aagacgcccg tgcattttac
      541 gaagaacttt cgtatatgct gatgaaccaa atggcggcgc cgaattcacc ccagtggttc
      601 aacacaggcc tacattgggc atacggggtg gatggtccct cgcaaggaca ttattacgtc
      661 gatccgacaa ccggcaaact ggttaaatct aaatcgtcgt atgaacaccc gcagccacat
      721 gcatgcttta tccaagcagt cgacgacgat ctcgtcaacg ccggtggtat tatggatctt
      781 tgggtgcgcg aggcgcggct ttttaagtat ggttcgggca caggctctaa cttctctgca
      841 ctcaggggag aaaacgaacg cctctcgggt gggggaaaga gctcaggcct catgagtttt
      901 ttgaaaatcg gcgacaccgc tgccggtgcg attaaatcgg ggggcactac ccgccgcgca
      961 gccaagatgg tctgcctcga catcgatcac cccgacgtgg aagcgtttat cgactggaaa
     1021 gtcaccgaag aagccaaagt cgcgagcttg gtttcaggtt ctaaagccat ccgccgcgtg
     1081 ataaatgagg ttttcagtgc agtggcaggt tgcgtgaaac aacctgcgct cctcgacccc
     1141 aaacaaaacc atatacttgc cctcgcgctt aaaaaggcac gcgcttcaag cgtccccgat
     1201 agctatgtac agcgtatcat ccatttagcg cggcaaggtt ttacttcgat tgagtttgaa
     1261 gagtacgaca ccgattggac atcgaaagcg tacctcaccg tggccgggca aaactcgaac
     1321 aactcgctgc ggctttctaa cgcatttttg aaggcagtgg agtcgaatgg cgattgggag
     1381 ctcatccgcc gcatcgatgg taaagtacac aagacgcttt ccgcacgcga ccttttcgat
     1441 aagctttcct ttgccgcgtg gtcgtgtgcc gatccgggtt tgcagttcga cactaccat
//
LOCUS       DAOMPD010000601         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31152_length_1498_cov_2.343728, whole genome shotgun sequence.
ACCESSION   DAOMPD010000601 DAOMPD010000000
VERSION     DAOMPD010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31152_length_1498_cov_2.343728"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1498)
                     /locus_tag="PLY93_14645"
     CDS             complement(<1..>1498)
                     /locus_tag="PLY93_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70761.1"
                     /translation="AGMALRTSTTWSWEVDFPGTYTVRVNDGSVDCSAGTVIASGPVV
                     MNTTVTTTIQDTDLLVQLNSIKVCFVPQGGGSTVVSSTKSVWRIEPQNVNTTYAKVNF
                     NRGEIPTLALADIFKPTTAIKEIRFICPAGSTGVDTPACTQPVPSGTNLQPQGAGSVE
                     VNTAYTLPTSSRVSTVAYDVRITLCDTSPTCAASAPPVFGTETAAPYAGSGGATIKVY
                     FLQDKTAGNSIFVATTGNDTYPGTNRLQPKKTLSAAVAVASGGKAIYVVGGNYCSVGD
                     IASCLPTAGALSLPAGTSIYGGFTSGWYRPDVATNRAVITAGSNASAHSIGVSVGAVN
                     TPVFVEGLDVTARRATADLTGGYNTIGLRAVSGTSTLTLYKNKITAEGDIALSPGPNP
                     GGSYGAVFNNFTAGGTVILDTNTIIAGQGWRGLSGGGGGATGAGGGTGGNGQGGIEGC
                     VACCNDSTWHAPGGAGGTGGAGTSNRGGNGGSGGYQDDGGSGCGARGGC"
BASE COUNT          341 a          442 c          372 g          343 t
ORIGIN      
        1 cgcaaccacc acgagcgccg caacccgaac caccatcgtc ttggtatcca ccgctaccac
       61 cattaccgcc acgattagag gttcctgcgc caccggttcc tcctgcacct cccggtgcat
      121 gccacgttga atcattgcaa caagcgacac agccttcaat acccccttgg ccatttcctc
      181 ctgtaccgcc accagcacct gttgcacccc caccgccacc gcttaatccg cgccagcctt
      241 gacctgctat gatcgtgttc gtgtcgagga tcacagtgcc ccctgctgta aaattattaa
      301 acactgcgcc gtacgaaccg ccgggattgg gtcccggaga aagagcgata tccccctctg
      361 cggtaatctt gttcttgtag agtgtcagcg tcgacgtgcc cgatactgcg cgtaggccaa
      421 tagtattgta ccctcccgtt aaatctgccg ttgcacgccg tgcggttacg tcaagcccct
      481 cgacaaaaac aggcgtgttg accgcgccga cgctcacacc gatgctgtgt gcagaggcat
      541 tactgcctgc ggtaatcacc gcgcggttgg tcgccacgtc gggacggtac cagccgctcg
      601 tgaaaccgcc ataaatactc gtcccagcag ggagtgaaag tgcgcctgca gtgggtaaac
      661 aagaagcaat atcacccaca gaacaataat ttccccccac cacgtaaatc gccttaccac
      721 cagaggcaac ggcaaccgca gcgctcaatg ttttcttggg ttgtaaacga tttgtgcctg
      781 ggtacgtatc gtttccggtt gtagcgacga aaattgagtt ccctgctgtt ttgtcttgca
      841 agaaatagac tttaatggtt gcaccacccg accccgcgta aggggctgcg gtttctgtac
      901 caaaaacagg aggggcgctt gctgcacagg tgggtgaggt gtcacacagg gtgatacgaa
      961 catcatacgc cactgtggag acgcgcgacg aagtgggcag ggtgtacgct gtgttcactt
     1021 ccaccgagcc agcgccttgt ggttgtaaat ttgtacctga cggtacaggc tgtgtgcatg
     1081 caggggtatc cacaccggtg gagcctgcgg gacagataaa tcgaatttct ttaatcgccg
     1141 ttgtcggttt gaaaatatcg gcgagcgcca acgtgggtat ttcgcctcgg ttaaaattga
     1201 ctttagcata agtggtgttc acgttttgcg gttcaatgcg ccatacagac ttcgtcgacg
     1261 aaaccacggt actaccgcca ccttgtggca caaaacagac tttaatcgaa tttaactgca
     1321 ctaacaaatc tgtgtcttgt atagtcgtcg taactgtcgt gttcataacc actggcccgg
     1381 aagcaatcac tgtgccagcg ctgcaatcga cgcttccgtc gttcacgcgt acagtatacg
     1441 ttcccggaaa atccacttcc catgaccagg ttgtcgaagt gcgcaaagcc attcctgc
//
LOCUS       DAOMPD010000602         1496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31206_length_1496_cov_2.079112, whole genome shotgun sequence.
ACCESSION   DAOMPD010000602 DAOMPD010000000
VERSION     DAOMPD010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31206_length_1496_cov_2.079112"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLY93_14650"
     CDS             <1..236
                     /locus_tag="PLY93_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP70762.1"
                     /translation="LLLSATEEFAGKERAGSATGLLMLTGNAGGVVVGVTMDLVKSNP
                     KDWTNAIYLLLAAVAASLFLAFFVEESFHAKES"
     gene            220..873
                     /locus_tag="PLY93_14655"
     CDS             220..873
                     /locus_tag="PLY93_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRP70763.1"
                     /translation="MQKKAEIIYVYDAYCGWCWGISNTIVRLKNDFQEKFIFSAHPGG
                     LMTGKRIGPIGDFGAYIEKAMPRVTEVTGAIFSEAQKTLIRNRSTVMDSRVPAAAALY
                     IQEVDADVDTIDLAHTLLALNFQTGVDISKPENYVQIFQKFNVDAAAFFSKYNDGFFE
                     EKVGDEFDFVRSTFGAESFPTLVYGQNGSYFPLCQGYQSYENLAPALETLYREPPPL"
     gene            910..>1496
                     /locus_tag="PLY93_14660"
     CDS             910..>1496
                     /locus_tag="PLY93_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70764.1"
                     /translation="MTPPNQRNKAIRAARDFFHKRQLIEVMTPKAVLSPALEPYMDAV
                     WLYSQHNTKQLFLATSPEMALKKRFQSEMKVNPTAQGIYEIAPVFRDDRKSKNHNIEF
                     TMVEWYEKDSSLNKILSEAAALIQNLGEYLTHKKIFPAYEIFTVEELFRQAGHSFTLT
                     SEEAVVEKYQSLYATLPQHFNAMDKATICFNLLFDA"
BASE COUNT          420 a          352 c          330 g          394 t
ORIGIN      
        1 cgcttttact ttcagccacc gaagaatttg ccggtaaaga gagggcgggt tctgccacgg
       61 gactccttat gctcaccggc aatgcgggtg gcgtcgttgt cggcgttaca atggatttag
      121 tcaagtcgaa ccccaaggat tggacaaacg ccatctatct actcttagcg gcggtagcag
      181 cctcgctttt tttagcattt tttgtagagg agtcgttcca tgcaaaagaa agctgagatc
      241 atctacgtgt acgacgcgta ctgcggttgg tgttggggca tctcgaatac catcgtgcgc
      301 ctcaagaatg atttccaaga aaaatttatt ttttccgcgc atccaggtgg acttatgacg
      361 ggaaaacgca ttggtcccat tggtgatttt ggtgcgtata tcgaaaaagc aatgccacgt
      421 gtcacagaag taaccggcgc tattttttct gaagcgcaaa aaacactcat ccgcaaccgt
      481 tcaacagtaa tggattcgcg cgtaccggcg gcggcggcac tctatattca agaagtcgat
      541 gcagatgtag atacgataga tttagcgcac acgctcttgg cgctcaactt tcaaaccggt
      601 gtcgatattt caaagccgga aaactacgta caaatttttc aaaagtttaa tgtcgacgcg
      661 gctgcatttt tttcaaaata caacgacggc ttttttgaag aaaaagtagg ggatgagttt
      721 gatttcgtgc gttccacatt cggcgccgag tctttcccca cactggtata cgggcagaat
      781 ggctcgtatt ttccactctg ccaaggttac caaagttatg aaaatttggc tccagcgctt
      841 gaaacgctat accgcgaacc cccaccgctt taatgttgtg gtacaaatca aatacattcc
      901 ttccttgtga tgaccccacc caaccagcgc aataaagcca tccgtgcagc gcgtgatttt
      961 tttcataaac ggcaactcat cgaggtcatg acccccaaag cagttttatc ccccgcgctg
     1021 gagccgtata tggacgccgt ttggttgtat tctcagcaca acacaaaaca attattttta
     1081 gcgacatcgc ccgagatggc gcttaaaaaa agatttcaaa gcgagatgaa ggtaaatccc
     1141 actgcgcaag ggatttacga aattgcgcct gttttccgcg acgacagaaa aagtaaaaat
     1201 cacaacattg aatttacgat ggtcgagtgg tatgaaaagg actcctcgct taataaaatt
     1261 ttatcagagg cagccgcact catccaaaat ttaggcgaat atcttaccca taaaaaaata
     1321 ttcccagcgt atgaaatctt tacggtcgaa gaacttttta ggcaagcagg gcattcattt
     1381 actttaacaa gcgaagaagc cgttgttgaa aaataccaaa gcctctacgc cactttgccg
     1441 cagcatttca acgccatgga taaagcgacg atttgtttca atttactttt tgacgc
//
LOCUS       DAOMPD010000603         1494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31245_length_1494_cov_2.340514, whole genome shotgun sequence.
ACCESSION   DAOMPD010000603 DAOMPD010000000
VERSION     DAOMPD010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31245_length_1494_cov_2.340514"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..633
                     /locus_tag="PLY93_14665"
     CDS             145..633
                     /locus_tag="PLY93_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70765.1"
                     /translation="MNWYLTALVVHITCAAIWIGGNVFFVSLIRLLGKKSEFRELRGE
                     IIYAYVMAYRLATYILFSLILLSGLLLIYERGLLNSLLWKSSLGLMALMKLSVFTALM
                     GLQMSHDFFFGPKAFTNKDGHVALEERHRRANRIVGQVVFLVSFFMLIVGIFLSRGFS
                     FF"
     gene            649..1368
                     /locus_tag="PLY93_14670"
     CDS             649..1368
                     /locus_tag="PLY93_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRP70766.1"
                     /translation="MKIPELLLPAGSMHKLKVAIEYGADAVYCGVPRYSLRARENEFR
                     LQNLGEAVEFVRARGKKIYFTVNGIPRNSKIPGYSRYLKAMAELKPDGLIMADQGLIM
                     ITRDECPDLPVHISVQTNTINYASVEFWRRIGAKRVILSREVTLTEVREIREKCPAIE
                     LEVFVHGSICIAHSGRCFMSNYFGNRDANQGACNNACRDSYQIYLKNPSSCRTLRMAI
                     KIPKKKTRRWNSSHPKTARSS"
     gene            1365..>1494
                     /locus_tag="PLY93_14675"
     CDS             1365..>1494
                     /locus_tag="PLY93_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRP70767.1"
                     /translation="MNSKDLRAIEFVEELADIGIDSIKVEGRTKNDYYAAMIARSYR"
BASE COUNT          374 a          321 c          367 g          432 t
ORIGIN      
        1 aaaattaaaa aaaagggtag gtataataaa taacatttct tgacccatca agttttcctt
       61 aagcgacttt cgacaaatta gagacataaa aatcgatttt tctgtaatgt ctctcgattt
      121 atttcgctat gccgcaaggc atttatgaat tggtatttaa cggcactcgt tgtgcatatt
      181 acatgcgctg caatatggat tggtggcaat gttttttttg tgagtctcat tcgcctctta
      241 ggtaaaaaat cggagttccg cgaactaaga ggggagatta tttatgctta tgtgatggcg
      301 tatcgtttag caacgtatat tctttttagt ctgatattgc tttcgggact tctgctgatt
      361 tacgaacgcg gtttactcaa ttcgttgttg tggaaatctt ccttggggct tatggctctc
      421 atgaagttaa gcgttttcac tgcactcatg ggcttacaaa tgtcgcatga tttctttttt
      481 gggccgaaag ccttcacaaa taaagacggg catgttgctc tggaagaaag gcaccgtcgc
      541 gcgaaccgca ttgtagggca agtcgtcttt ttggtgtcgt tctttatgct catcgtcggt
      601 atttttcttt cacgaggatt ttcttttttt taggttgatt atattcccat gaaaattcca
      661 gaacttctgt tgcctgcggg cagtatgcac aaattaaaag tcgcgattga gtacggcgcc
      721 gatgcggtgt actgcggtgt gccgcgctat tcgttgcgtg cgcgcgagaa cgagttccgt
      781 ttgcaaaatt taggcgaggc agtcgagttt gtgcgtgcac gcggcaagaa aatttatttc
      841 acagtgaacg gtatcccgcg caattcaaaa attcccggtt actcgcgtta cttaaaagcg
      901 atggcggagc tcaaacccga tggcctcatc atggcagacc aaggcctcat tatgattacg
      961 cgcgacgagt gtcccgactt gccggtgcat atttcggtgc agacgaacac cattaactat
     1021 gcgtcggtgg agttttggcg gcgcattggc gcgaagcgcg tcattttgtc gcgcgaggtt
     1081 actctcaccg aagtgcgtga aatccgcgag aagtgcccag cgattgaatt ggaagttttt
     1141 gttcacggtt caatttgtat tgcgcacagt ggtcgttgtt ttatgagtaa ttatttcggc
     1201 aaccgcgacg ccaaccaagg cgcgtgcaac aacgcgtgcc gcgactcgta ccaaatttat
     1261 ttgaaaaatc cgagcagttg caggacactc cggatggcca tcaaaatccc gaaaaagaag
     1321 acgcgcagat ggaactcatc acatccgaag acggcacgtt cctcatgaac tcaaaagatt
     1381 tgcgcgcgat cgagtttgtc gaagaacttg ccgacatcgg cattgattca atcaaagtcg
     1441 agggccgcac caaaaacgat tattacgcag cgatgattgc gcggagttat cgca
//
LOCUS       DAOMPD010000604         1491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31328_length_1491_cov_1.835655, whole genome shotgun sequence.
ACCESSION   DAOMPD010000604 DAOMPD010000000
VERSION     DAOMPD010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31328_length_1491_cov_1.835655"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1491)
                     /locus_tag="PLY93_14680"
     CDS             complement(<1..>1491)
                     /locus_tag="PLY93_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRP70768.1"
                     /translation="ALQAHSDVSDASTIQTLIADMNAGNVDCLVIDRANPVYEMPASS
                     GFTEALKKVKTVIYIGDHLDDTAVLATHFLPVSHSLESWSDALSHGSYSIVQPVIRQL
                     FDTLPVLEIYGRLTGNTTSAYEQVKKSHTANAKGASWDSTLSKGYTIVEAAGIGAARS
                     FKSAALKDVKPSLEPKAGYTLNLIESVGLGDGVGGNISFRHELPDVVTKITWDNVLLM
                     SPADAKTLGIKTQDLIKVGNGKTSFEIPAFVQPGMRRGSLAVAIGFGQKNIGKVGANV
                     GQNAYALQGFHNGQVSSSGLAVTIEKVGKYDMLASTQRHYEFAVIRGLARQVTLPTFE
                     KAVKEKNYHLLHEHEEKPFGGKAKGLYTPHNHSEGYRWNLNIDLNKCTGCSACVMACY
                     SENNIPAVGKAEVNRGREMSWLRIDRYYIYENDAAEKADADMVAPDVFFQPVMCQHCE
                     NAPCENVCPVGATGHTQEGLNYMAYNRCIGTRYCSNNCPYKVRRFN"
BASE COUNT          339 a          355 c          393 g          404 t
ORIGIN      
        1 cagttaaaac gacgcacttt gtaagggcag ttgttcgagc aataacgagt accaatacaa
       61 cggttgtacg ccatgtagtt taagccttct tgtgtgtggc cggttgcacc gacggggcac
      121 acgttttcac acggtgcgtt ctcgcagtgt tggcacatga cgggctggaa gaaaacatcc
      181 ggtgccacca tgtcggcgtc ggccttttcc gcagcgtcgt tttcatagat atagtaacgg
      241 tcgatccgca accagctcat ttcgcggcca cggttgacct ctgctttacc gacggcgggg
      301 atgttatttt ctgaataaca cgccatcacg cacgcagagc aaccggtaca cttattgagg
      361 tcgatgttga gattccaacg gtagccttcg ctgtggttgt gcggagtgta aagacccttg
      421 gctttgccac caaaaggttt ttcctcatgc tcgtgtaaga gatgatagtt tttttctttg
      481 accgcctttt caaaagttgg taaagtgact tggcgcgcca aaccacggat gaccgcaaat
      541 tcgtagtggc gttgtgtcga cgccagcatg tcgtatttgc ccactttttc aatcgtcacg
      601 gcaagtcccg atgaagaaac ttggccgttg tggaagcctt ggagtgcgta agcgttttgg
      661 ccaacatttg cacccacttt accgatgttc ttttgaccaa agccaatcgc cactgctagc
      721 gaaccacggc gcatgccggg ttgtacaaat gcggggattt caaacgaggt tttgccatta
      781 ccaactttaa ttaagtcttg ggtcttgatg cccaacgtct ttgcatcggc aggactcatg
      841 agaaggacgt tgtcccaagt gattttagtg accacatcgg gtagttcatg gcggaacgaa
      901 atgttgccac ccacaccgtc acccaagccc acgctttcaa taaggttgag agtgtaacct
      961 gctttgggtt ccaacgacgg ttttacgtct ttgagtgcgg cagatttgaa tgagcgcgct
     1021 gcaccaatgc ccgccgcttc aacgattgta tatcctttag aaagagtcga atcccatgaa
     1081 gcacctttgg cgtttgccgt gtggcttttc ttgacttgtt cgtacgccga ggtggtgttt
     1141 ccggttaaac gaccgtagat ttctaatacg gggagtgtat caaaaagctg acggatgacg
     1201 ggttgcacaa tgctgtacga accgtgtgag agagcgtctg accaactttc gagtgaatgg
     1261 cttacgggta aaaagtgtgt cgctaaaacc gcagtatcgt cgaggtgatc gccgatatag
     1321 atgactgttt ttactttttt gagcgcttcg gtaaaaccag aacttgcagg catttcataa
     1381 acagggttgg ctctgtcgat gacgagacaa tccacgttgc cggcattcat gtcggcgatg
     1441 agcgtttgaa ttgtcgaagc gtcgctcaca tcgctgtgtg cttggagtgc c
//
LOCUS       DAOMPD010000605         1484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31501_length_1484_cov_2.517145, whole genome shotgun sequence.
ACCESSION   DAOMPD010000605 DAOMPD010000000
VERSION     DAOMPD010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31501_length_1484_cov_2.517145"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1484)
                     /locus_tag="PLY93_14685"
     CDS             complement(<1..>1484)
                     /locus_tag="PLY93_14685"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRP70769.1"
                     /translation="SYDENAARHKWSYATLYKKVAAFVETLKSKGIKPRDTLAAFMPN
                     TEETLVTLLATVSCGATFSSTSPDFGVGAASDRFTQAHPRFLTASAHYYFKGKKIDVV
                     DKLIEIVRKATSIETLFLFRATEADLARLANELPQLKIVPVSTENEVAHSITFEPYSF
                     QDALYIMYSSGTTGLPKCIVQGHGVILNQMKEHLLHCGSTEDDIIFYYTTCGWMMYNW
                     LVSALTFGATLVLFDGNPFHHGSDLMFKLALETQMTVFGTSAKYLTTLMQHNFDAQEL
                     TQNSKIRLLLSTGSVLPPEGFHYVYEHIHPTVQLASISGGTDLNGCWALGNPDLPVRV
                     GELQSRALGLSVEISNDVGESVVGEMGELVCTKPFPAMPLGFLNDADGARYKASYFER
                     FEGQDVWAHGDFAEITPEGGVIIGGRSDATLNPGGVRIGTAEIYRVLELMPFIEDSLV
                     IGRPVDASEEVILFLKLREGTLTHDQIAEVKKVIREKVSPRHVP"
BASE COUNT          387 a          377 c          364 g          356 t
ORIGIN      
        1 cggggacgtg acgcggcgag accttctcgc ggatgacttt ctttacttct gcaatttggt
       61 cgtgcgtaag agtcccttcg cgcagtttga ggaaaagaat cacttcttca gaggcatcca
      121 ccggacgccc aatcacaagt gaatcttcaa taaatggcat gagctccaac acacgataaa
      181 tctctgccgt gccaatgcgt actccccccg ggttcaaggt ggcatcgctg cgccccccga
      241 tgatgactcc accttcggga gtaatctcag caaagtcgcc gtgcgcccac acatcttgac
      301 cctcaaaacg ttcaaaatac gacgccttgt agcgtgcgcc gtcggcatcg ttcaaaaagc
      361 ccaacggcat cgcggggaaa ggttttgtac aaacgagctc gcccatctca cccacaacac
      421 tttcgcctac gtcgttagaa atctccactg aaagccccaa cgcgcgcgat tgcagttcgc
      481 ccacgcgcac cggtaaatca ggatttccca acgcccaaca accattgaga tcggtgccgc
      541 cagaaatcga cgccaactgc acagtgggat ggatgtgctc atacacatag tgaaaccctt
      601 cgggaggaag caccgaccct gtcgaaagca aaagtcgtat tttagaattt tgagtgagct
      661 cttgcgcatc aaaattgtgt tgcatgagcg tggttaaata ttttgcgctc gtgccgaaga
      721 ctgtcatttg ggtttcaagt gcgagcttaa acatcaagtc ggagccgtgg tggaatggat
      781 tgccgtcaaa aagaacaagc gtggccccaa aggtgagagc gctcaccaac cagttataca
      841 tcatccaacc gcaagtggta tagtaaaaaa taatatcgtc ttcggtagaa ccgcaatgca
      901 acagatgctc tttcatctgg ttcaagataa cgccgtggcc ttgcacgatg cacttgggta
      961 gtcccgttgt gcccgacgaa tacataatat atagtgcatc ttggaaagag tacggttcaa
     1021 aagtaattga atgcgcaact tcattttctg ttgaaacagg aacaatcttg agctgcggta
     1081 attcatttgc gagacgggct aaatccgcct cggtggcgcg gaaaaggaag agtgtttcaa
     1141 tcgacgtagc tttgcgtaca atttcaatga gcttatcgac cacatcgatt ttttttcctt
     1201 tgaagtaata atgtgcgctc gcggtcaaaa aacgtgggtg tgcctgagta aatcggtcgg
     1261 acgcggcgcc gacaccaaaa tcgggggaag tggagctaaa cgtggcgcca cagctcaccg
     1321 tcgctaaaag tgtaacgagc gtttcttcgg tgttgggcat aaacgcagca agagtatcgc
     1381 gcggctttat acctttgctt ttcaatgttt ctacaaacgc tgctaccttt ttgtaaagtg
     1441 tagcataaga ccatttgtgg cgtgcggcat tttcgtcgta gctc
//
LOCUS       DAOMPD010000606         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31612_length_1480_cov_2.239298, whole genome shotgun sequence.
ACCESSION   DAOMPD010000606 DAOMPD010000000
VERSION     DAOMPD010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31612_length_1480_cov_2.239298"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..992
                     /locus_tag="PLY93_14690"
                     /pseudo
     CDS             18..992
                     /locus_tag="PLY93_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1083..>1480
                     /locus_tag="PLY93_14695"
     CDS             1083..>1480
                     /locus_tag="PLY93_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRP70770.1"
                     /translation="MQKQVITIDGPAGSGKSTVAYEVAEALGFNQLDSGALYRAFTYL
                     GYRKFEQLAMENQGNGGGDNYPIAMENQRWADAIQQNTALVQYLKEVPLRLEFSRNGK
                     QHVFHGEQELAEELRSSAVASRIKPIADTL"
BASE COUNT          362 a          349 c          420 g          349 t
ORIGIN      
        1 gtttacgcac ccgccccatg cggcgcgtaa cccaaccgct ggctgaattg ggtgcacagt
       61 ttgaatactt aggaaagccg gattgcctac ctatacgagt aacaggtgtt cgtctcggcg
      121 caaaatacgc cgcgcatagc ggagaggcaa gcgccgatct cccgcttcgg gaacgtcctg
      181 cggtgagcgc agtcgaaccg agtcggggca tgatgtttac agaaacctta ggttcggcgc
      241 aggtgaaatc tgccgtcatc ttggctgcgt tcgcatcggg agtcgcactc gaacttattg
      301 aagaaatccc ttcgcgcgac cacaccgaga atatgctgcg ctttgctggt gtggacatag
      361 ttaaagagac aatagacacg gatgaacacg gacgaacacg gattccgggt gagacatacc
      421 gtattcgttt agcggcagag cagcgtgttt tgccgcgcga atataaaatt tggggagaca
      481 tttcgtcggt ggcgtttttt gctgtaggcg ccagtctttt acccggaagc gagttgttgc
      541 ttcgtggcgt gcttttaaat acttatcgtg accgttatct ttacacgctc aaagaaatgg
      601 gcgccgcaat taccattttg ccgcaacgcg aggaatgtgg cgagaagggc ggcgatattc
      661 tggtgcggca tgcgccactc aaagggataa ctatccctaa aaaagacatc cccgcgctca
      721 tcgacgaatt gccgatattg actgtcgccg gcgcttttgc cgagggggat ttcacatacc
      781 gcgacgccaa agagttgcgc gtgaaggaat ccgaccgcat cggcattatg gccgccaatt
      841 tgcgtggcgt gggtatcgag gtcgaggagt ttgacgatgg ccttcgtgtt gtgggcatgc
      901 cacagcagtc gcttgttggc agcattgtaa caaaaatgga ccatcggatt gtgatgtcgt
      961 ttgagattgc gaaccttgtt caccaaagcc agttgactat cgaaggcaag gagtgggtcg
     1021 aaacgagttt tccggcgttt tatagtaaat tagcatcggt tctggagtat aagccgatac
     1081 cagtgcaaaa acaggtaatt accatcgatg gtccggcggg ttcggggaaa tcaaccgtcg
     1141 cgtatgaggt cgccgaggcg ctggggttta atcagctcga ttcgggagcg ctctaccgcg
     1201 cgtttacgta cttgggatat agaaaatttg aacagttagc tatggaaaat cagggcaatg
     1261 gtggggggga taactatccc atagctatgg aaaaccagcg ctgggcagac gccatccaac
     1321 aaaacacagc tcttgttcag tacttaaagg aagtcccact ccgtttagaa tttagccgaa
     1381 acggtaaaca gcacgtgttc catggtgagc aagagttggc tgaagaactt cgttcgagtg
     1441 ccgttgcatc acgtatcaaa ccaatcgccg atactctttt
//
LOCUS       DAOMPD010000607         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31672_length_1478_cov_2.670415, whole genome shotgun sequence.
ACCESSION   DAOMPD010000607 DAOMPD010000000
VERSION     DAOMPD010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31672_length_1478_cov_2.670415"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLY93_14700"
     CDS             complement(<1..794)
                     /locus_tag="PLY93_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70771.1"
                     /translation="MATQRTIPLILSCVILVFIATRSTLYAQAEFPKIPHIADDADGK
                     NEKKEKTKLKPSTALTDEKGMIAIPPRDQSTENPLSAGGLDRTGEWVPEFERQFAIRK
                     DKPDFEFLWGFNMLQEFHGYNNADLRKLNSTNDYQIRTTDDAQGLALTRAKFDTSFMF
                     PKQRVGLEMSFGFDGVWGSFQLQGNGNPGTRIARANVFWEFFNLDGFSIDTVIGRQFY
                     SIGGIQNDYMLRDILDAIVFNVHWKNMLDVKILGLDIYSGANSYGAG"
     gene            1046..>1478
                     /locus_tag="PLY93_14705"
     CDS             1046..>1478
                     /locus_tag="PLY93_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRP70772.1"
                     /translation="MLLNTADAKKEKVQNYVAQMCTIFESGKYPLVCVTRPWSKYNPE
                     ISGEFSRPHALKAYLTREFQSLLDLDATIKISASRTKRDLYDPALFAKLSEDDWDLRQ
                     KKLFLFTPERIDISLNRLEHYTGTRADDFQKYILFTNYRMHI"
BASE COUNT          405 a          339 c          309 g          425 t
ORIGIN      
        1 cccgcgccat aactgttcgc tcctgaatag atgtcgagtc ccaaaatttt gacgtccaac
       61 atatttttcc agtgcacatt gaatacaatt gcgtctaaaa tatcgcgtag catgtaatcg
      121 ttttggatgc caccgatgct ataaaactgc cgaccgatca cggtatcgat agaaaaacca
      181 tctaaattaa aaaattccca aaaaacgttt gcgcgtgcga tacgcgtacc cgggttgcca
      241 ttgccttgga gttgaaagct accccacacg ccatcaaaac caaaagacat ctcaagcccc
      301 acacgctgtt taggaaacat aaacgatgta tcaaatttcg cgcgtgtgag cgcaagtccc
      361 tgtgcatcgt cggtggtgcg gatttgataa tcgttcgtgc tgttgagctt gcgcaaatcg
      421 gcgttgttat acccgtggaa ctcttgcagc atgttaaagc cccacaaaaa ttcaaagtcg
      481 ggtttgtctt ttctaatggc aaactgtcgt tcaaactcgg gtacccattc accggtgcgg
      541 tcaaggccac cggcggaaag tgggttttca gttgattggt ctctgggtgg aatggcaatc
      601 attcctttct cgtcggtgag tgcagtactc ggttttaatt ttgtcttttc ttttttctca
      661 ttttttccat cagcgtcgtc agcgatatgg gggattttag ggaactctgc ctgcgcgtag
      721 agcgtcgatc gagtagcgat aaaaaccaaa ataacgcaag aaaggatgag ggggattgtt
      781 cgttgtgttg ccataatgtt tactatttgt aagagtaaat cctatttatt aaagtgagtg
      841 tatgcgatga agcaaaaatc tttaatccga ctcgttttac actctcgcat aagagcttgt
      901 gcgccaattt actgaggcat tttttttata aattttggta cacactgtat gagttgccgt
      961 acaaactcat ggatatatga atgctgcggt gaagataatg ccaaaattgt cttgccgccg
     1021 cttgaaatta ctcttaagat accgtatgct gctcaacacc gccgatgcca agaaagaaaa
     1081 agtccaaaat tatgttgcac aaatgtgtac aatttttgaa agtggcaaat accctttggt
     1141 ttgcgttaca cgcccgtggt cgaagtataa tcctgaaata tcaggcgaat tttcgcgccc
     1201 ccacgcgctc aaagcgtact taacgcgtga atttcaatcg ctactcgact tagacgccac
     1261 gataaaaatt tcggcatcgc gcaccaagcg cgatctttat gaccctgccc tttttgctaa
     1321 actttctgaa gacgattggg atttacgcca aaaaaaactt tttttattca ctcccgaacg
     1381 catcgatatt tcgctcaatc gcctcgaaca ctataccggc acacgcgctg acgattttca
     1441 aaaatatatt ctttttacaa actaccgcat gcacattg
//
LOCUS       DAOMPD010000608         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31705_length_1477_cov_3.095640, whole genome shotgun sequence.
ACCESSION   DAOMPD010000608 DAOMPD010000000
VERSION     DAOMPD010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31705_length_1477_cov_3.095640"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLY93_14710"
     CDS             complement(<1..567)
                     /locus_tag="PLY93_14710"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HRP70773.1"
                     /translation="MDKRTKLVLIISVVLLVLLWFFWPSSSGTKGDKALKAQTREAAL
                     SANPQSGEYRQFVEQNRNLFTEANMYSFDQLMEMARSGRLSLVAELWKMRTKCEGGPI
                     TTDENAVPKRTMSADECNIRIENFLREQYPPPANEKMIALFRTYLRYEDAMRTLHLTE
                     SMPLSERLEIMKKKRREFFSESDARLVFG"
     gene            complement(597..>1477)
                     /locus_tag="PLY93_14715"
     CDS             complement(597..>1477)
                     /locus_tag="PLY93_14715"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP70774.1"
                     /translation="AMLWAAVIGIIDEQDGLLYYINAEHPWVAVYRGGSAFFIENELS
                     LHKLGMPENKETFRVKTFKLESGDKVFSGSDGRDDIALGKDQHGNTSINTDEKLFLRV
                     IEKAHGELGGIVDELKSLGSITDDLSLVRIEFDPKDFKRLDKSERKRIQEKILVNNKN
                     QDIEKVKKGYEENPHRLDLLYELAFLSAHRQDWQIAAEFAEKFVALDASDKAIVYLAA
                     QALKLSAKGDVGILKRAADYAERLLLRVPNDVNVILNLADIERYLGRHEKMQNLLLAA
                     KAIDPTNKTLVKFRID"
BASE COUNT          372 a          370 c          296 g          439 t
ORIGIN      
        1 accaaaaacg agccgcgcat cgctttcgga gaaaaactca cgacgttttt ttttcatgat
       61 ttcgagacgt tcagaaagag gcatgctctc tgtgagatgg agtgtacgca tagcatcttc
      121 gtagcgcaga taggtacgga acagtgcgat cattttctcg ttagctggcg gaggatattg
      181 ttcgcgtaaa aaattttcaa tacgtatatt gcattcatct gcgctcatcg tgcgcttagg
      241 cactgcattc tcgtctgtcg taatcggccc tccttcgcac ttcgtgcgca tcttccaaag
      301 ctcggccacg agtgaaaggc gccctgagcg tgccatctcc ataagttggt cgaaagaata
      361 catattggcc tcagtgaaaa gattacgatt ttgttctaca aactgccgat attctcctga
      421 ttgtgggttt gccgacaacg ccgcttcacg cgtctgcgct ttgagcgcct tatctccttt
      481 tgtgccgctt gagcttggcc aaaaaaacca caagagcaca aggagaacca cagaaattat
      541 taataccagc ttagtgcgtt tgtccatgcg tgcttgtttt aacgccacgc gccgcgtcaa
      601 tcgattcgga attttacaag tgttttattt gtggggtcga ttgcttttgc tgcgagcaaa
      661 aggttctgca ttttttcatg ccgccctaaa tatctttcaa tgtcggccaa attgagaatt
      721 acattgacat cgttgggtac gcgcaaaagc aaccgctccg cataatctgc agcgcgcttc
      781 aaaataccaa catctccttt cgcgctcaat tttagcgctt gtgcggcaag ataaacaatc
      841 gctttgtcgg aagcgtcaag cgcgacaaat ttctcggcaa attccgcagc gatttgccaa
      901 tcctgtcgat gcgccgatag aaaagcgagc tcataaagta aatcgaggcg gtgcggattt
      961 tcttcatacc cttttttaac tttttcaata tcttgatttt tgttatttac taaaattttt
     1021 tcttgaattc tttttctctc gcttttgtct aaacgcttaa agtcttttgg atcaaattcg
     1081 atacgtacca acgagaggtc gtcggtaatg cttcccaacg attttaattc atctacaata
     1141 ccgcccaact caccgtgcgc tttctcaata acccgcaaaa ataatttttc atcggtgttg
     1201 atactggtat tgccgtgctg gtctttgcct aatgcaatat cgtcgcgtcc atccgagcca
     1261 gaaaaaactt tatctccgct ttccaactta aaagtcttca ctctaaaagt ttctttattt
     1321 tcgggcatcc ctaatttatg caaactcaat tcattctcga taaaaaacgc cgagccacca
     1381 cgatacaccg ccacccatgg atgctcggcg ttgatataat aaagtaatcc atcttgctcg
     1441 tcgataatgc cgatgaccgc cgcccacagc atcgccc
//
LOCUS       DAOMPD010000609         1477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31709_length_1477_cov_2.272855, whole genome shotgun sequence.
ACCESSION   DAOMPD010000609 DAOMPD010000000
VERSION     DAOMPD010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31709_length_1477_cov_2.272855"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLY93_14720"
     CDS             <1..473
                     /locus_tag="PLY93_14720"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP70775.1"
                     /translation="TGSLMMGTVGSQDRLDTTVIGDTVNLASRVESLTKIYGVRILMT
                     DAMLRSIENPQKYNTREIDSVIVKGKTKPVVLYEVFNAEPEEIRTAKNQILTDFAQGL
                     ALYKSAAFAQAKNAFETCLKTCPWDKPSQVYWQRCKKLIETPPGAKWTGVVKLK"
     gene            512..>1477
                     /locus_tag="PLY93_14725"
     CDS             512..>1477
                     /locus_tag="PLY93_14725"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRP70776.1"
                     /translation="MRFTRLYRLFLILLSPLLILNCGQLLDIMNELDKQEATAPNTNL
                     GEPGKWTTLVYLDADNNLEGAGVADVQEMISRAPQYSGNKVYVLMDRIGGYSSASMPD
                     GFGNFTGARLFEVTSTGTLTPVSPGVFLSLGSNDGTTEINMGDKDTLENFIKWGLAQA
                     DADGSKYVFLDIWDHGAGWGGNAYGGNAVAWDDTNSHDALSITEIRTAITNAHAAGTS
                     NGKRVTILGFDACYMGTAENAFAFKDLVSIMVGSEETEPGNGWDYSKWLPSGDTSPAD
                     LGSQLVTSYGSYYNGSGNNITLSAYNLSQIPALEASINKFVDSLVG"
BASE COUNT          393 a          385 c          371 g          328 t
ORIGIN      
        1 acacaggctc tctcatgatg ggcacggtag gttcgcaaga ccgcctcgac accacggtga
       61 tcggcgatac ggtcaacttg gcatcgcggg ttgagtcgct cacaaaaata tacggcgtgc
      121 gtattttgat gaccgatgca atgctccgct cgattgaaaa tccacagaag tacaacacgc
      181 gcgagatcga ttctgttatt gtgaaaggga aaacgaaacc cgtcgtttta tacgaagttt
      241 ttaacgccga accggaagaa atccgtaccg ccaaaaatca aattttgacc gactttgcgc
      301 aagggctggc gctctataaa tcggcggcat ttgcacaggc gaaaaatgcg tttgaaactt
      361 gcctcaaaac ttgtccatgg gataagccct cgcaagtgta ttggcaacgc tgtaaaaaat
      421 taatcgaaac cccgcccggc gcgaaatgga cgggagtggt aaaactcaaa taaaccattg
      481 acgtttgcaa cggcatgccg tagatgccgt tatgcgtttt actcgactgt atcgtctctt
      541 tctcatttta ctctcaccgc ttttaatcct aaactgcggc caactgctcg acatcatgaa
      601 cgaactcgat aagcaagagg ccaccgcgcc caacaccaac ttgggcgagc ccggcaagtg
      661 gacgacgctg gtgtacctcg acgccgacaa caatttagaa ggcgcgggtg tcgccgacgt
      721 acaagagatg attagccgtg cgccgcaata cagcggtaat aaagtctatg tgctgatgga
      781 taggattggt gggtatagca gcgcgtcgat gcctgatgga ttcggtaatt tcacgggtgc
      841 acgcctcttt gaagtaacca gcacagggac actgactccc gtatccccag gagtattctt
      901 gtctctcggt tccaacgacg gtaccactga aatcaacatg ggcgataaag acacccttga
      961 aaatttcatc aaatgggggc ttgcacaagc cgacgccgac ggtagtaaat acgtctttct
     1021 cgatatttgg gaccacggcg ccggttgggg cggcaatgca tacggcggca acgccgtcgc
     1081 gtgggacgac accaacagcc acgatgcgct ctccatcact gaaattcgta ctgcaattac
     1141 caacgcacac gccgccggca cctcgaacgg caaacgggtg acgattttag gttttgatgc
     1201 gtgctacatg ggcaccgccg aaaacgcatt tgcattcaaa gacttggtga gcatcatggt
     1261 gggctccgaa gaaaccgagc ccggcaacgg ttgggactat tcaaaatggt tgccttcggg
     1321 cgacaccagc cctgccgatt taggaagcca gcttgtcaca tcttacggca gttactacaa
     1381 tggtagcgga aataatatta ctctttccgc gtacaatctt tcacaaatac cggcattaga
     1441 agcatccatc aataaattcg tcgatagtct cgtcggc
//
LOCUS       DAOMPD010000610         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_31849_length_1472_cov_1.971771, whole genome shotgun sequence.
ACCESSION   DAOMPD010000610 DAOMPD010000000
VERSION     DAOMPD010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31849_length_1472_cov_1.971771"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>281)
                     /locus_tag="PLY93_14730"
     CDS             complement(<1..>281)
                     /locus_tag="PLY93_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70777.1"
                     /translation="SAPVNTSHSAEDDAATLARDSEEDAADKKDDKEEKKSGFKIPKP
                     FFFNMYMRASAQDDFGYNSPTLGWSPFYGRLMNETPWMMADFGYHVVRP"
     assembly_gap    282..291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(503..>1472)
                     /locus_tag="PLY93_14735"
     CDS             complement(503..>1472)
                     /locus_tag="PLY93_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70778.1"
                     /translation="RFGHHPKAYCYGADGDTFVAYETGCLALDTTYSTGFGYFEWSDL
                     FKRVSRRIVNDDTYLLNGDSPIDFGGALTGSAAKFNETWDGRQPYHAVNKITAHDGFT
                     MYDLLSYNGKRNGLGPLNPLGADPYSGDDGSNYSRDWGFNENLKRQNFRNLIALLMIS
                     NGSPMLLGGDEWMRTQFGNNNAYTAGADNQYNWFRWGVWLADPNAYRMFDFTRKMIQL
                     RKDNAWAFEKETYTDIRNDLSWHDVGASPNNCDANYASGAAWSGSDKVISLCYKNPPI
                     GKKEIFIAFNLGASATHTINLPTGTWNVVVNTETYFEGFHAQLLRE"
BASE COUNT          332 a          369 c          387 g          374 t
ORIGIN      
        1 ggggcgcacg acgtggtatc caaagtcggc catcatccac ggcgtttcgt tcatgaggcg
       61 accatagaac ggcgaccatc ccaaagtggg tgaattatat ccgaagtcgt cttgcgcact
      121 ggcgcgcatg tacatattaa agaagaacgg tttgggtatt ttgaacccgc tttttttctc
      181 ttctttatca tcttttttgt cggcggcatc ttcttcgcta tcgcgggcaa gcgtggctgc
      241 atcatcctct gcgctgtggg atgtattaac gggcgcgctc gnnnnnnnnn ntgcggcctg
      301 gttaaacgaa tacgcgtgtc gctttcaggg gcggcggtaa attgtgtggt gagcgtacac
      361 acaaagaaga cgacgaatac attgcggtac atagaattac tgcttctgta aaattaaaat
      421 tgtgcgcgcc ggtaatccat acgtgcctga cactgtggtt gcgttacact taggcgccga
      481 tggattaaat gtcgcttcac tgctattcgc gcaatagttg cgcgtggaag ccctcaaaat
      541 aagtttcggt attcacgacg acattccacg taccggtggg caggttgatg gtatgcgtcg
      601 ccgatgcgcc caaattaaac gcgataaaaa tttctttttt accaatgggg ggatttttat
      661 agcagaggga aatgactttg tcgctacccg accacgcagc gcccgaagcg tagtttgcgt
      721 cgcagttgtt gggcgatgca cccacgtcgt gccagcttaa gtcgttgcgg atatcggtgt
      781 aggtttcctt ttcaaacgcc cacgcgttgt ctttgcgtaa ctggatcatt ttgcgcgtga
      841 aatcgaacat gcggtacgca ttcggatcgg cgagccacac accccaacgg aaccagttgt
      901 actggttgtc ggcaccggcg gtatacgcgt tgttgttgcc gaattgggtg cgcatccact
      961 catccccacc caatagcatc ggcgaaccat tggaaatcat cagcagcgcg atgaggttac
     1021 gaaaattttg ccgtttcaaa ttttcgttaa agccccagtc gcgcgaatag ttactcccat
     1081 cgtcgcccga atacggatcg gcgccgagtg ggtttaatgg ccccaaccca ttgcgcttgc
     1141 cgttgtaact caatagatcg tacattgtga agccgtcgtg cgctgtgatt ttattcaccg
     1201 cgtggtatgg ttggcggcca tcccacgttt cgttgaattt tgccgcgctt cctgtgagag
     1261 ctccgccaaa atcaatcggt gagtcgccat tcaaaagata ggtgtcgtcg ttcacgatgc
     1321 gacgggagac gcgcttgaat aaatccgacc actcaaaata gccaaaaccg gtggaatacg
     1381 tcgtgtctaa agccaaacag cccgtttcat acgcaacaaa cgtgtcaccg tcggcgccat
     1441 agcaatacgc tttggggtga tggccaaagc gg
//
LOCUS       DAOMPD010000611         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32192_length_1459_cov_3.087607, whole genome shotgun sequence.
ACCESSION   DAOMPD010000611 DAOMPD010000000
VERSION     DAOMPD010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32192_length_1459_cov_3.087607"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..627
                     /locus_tag="PLY93_14740"
     CDS             235..627
                     /locus_tag="PLY93_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70779.1"
                     /translation="MKTGFVLIFLFLSLSFSLSAIPVAHPVVVKAKPLKGNPTTQAEL
                     KKVIILDFKNIDQNKNYNYLESSITEAIRNELKAKFDFQELNPRVWRMAAQKNYYQWP
                     SENYTKGFALHLGCRWVLPSTSHTKGSG"
     gene            563..1411
                     /locus_tag="PLY93_14745"
     CDS             563..1411
                     /locus_tag="PLY93_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70780.1"
                     /translation="MHCTWVVGGYYQALLTQKAAGESAYVLQAHVFVMDVGKRKVVGE
                     FTMAFDADATLFTRIEEMGTRVTQVAKAVLPNKGATGLTPVEFDEVGRDELGLLVGTG
                     VVSVPAAFSGSYDSDKPIFIKDFSTPVSAAIFYTRHDFLLPQFLLNTMVGVNFGSGNL
                     TIANDTKKISANALDIYVAAHLGYEFAFWRFSAAPLLGGGFSFASINLDYNTLSTLPL
                     TPSGEKRSSAKINASAPFAEGGVKLAFQVTKNIYLHAYALYRQSFYMSTSVGELFTLG
                     GLSMRF"
BASE COUNT          391 a          312 c          356 g          400 t
ORIGIN      
        1 ccaaggtgct ggagtggtcg aaaaagaggg taaattttga ggaaaaactc acttttagga
       61 gggctggaca ccgattcttt gtacccattg gtacgacata atcataaaaa attaaacacc
      121 tcttggacgg tgcacggatt ttttctatcc ccaaaaaaaa aagtattgac gcggccaaat
      181 aggcgtgttc ggtgtgttta tgcgcagagc aagtagggca taataactat gtcaatgaaa
      241 acaggattcg ttttaatttt tctttttctc tccctatctt tttctttatc cgcaatccca
      301 gttgcacatc ctgttgtagt aaaagcgaag ccattgaaag gcaatcccac cacgcaagcg
      361 gaactcaaga aagttattat tctcgacttc aaaaatatcg accagaacaa aaattataat
      421 tacttagaat cgtcgatcac agaggcaatc cgcaacgagc tcaaggcaaa atttgatttt
      481 caagaattga acccccgcgt gtggcgaatg gctgcgcaaa aaaattatta ccagtggcca
      541 agcgaaaact acaccaaagg gtttgcactg cacttgggtt gtcggtgggt attaccaagc
      601 acttctcaca caaaaggcag cgggtgaaag tgcgtatgtg ctgcaagcgc atgtttttgt
      661 gatggatgtc ggtaagcgga aggtcgtcgg cgaatttacg atggcattcg acgccgatgc
      721 gacactcttt acgcgcatcg aagagatggg cacgcgcgtg acgcaagtgg cgaaagcagt
      781 gctgccgaat aaaggcgcaa ccggccttac tcccgtcgag ttcgacgagg tggggcgcga
      841 tgaattgggg cttcttgttg gcacgggagt ggtatctgtg cccgctgctt tttcgggtag
      901 ttacgatagc gataagccca tctttattaa agatttttca acgcccgtga gtgccgcaat
      961 tttttatacg cgtcatgatt ttttactgcc ccaatttttg ctcaacacaa tggtgggcgt
     1021 caatttcggt agcggtaatt taaccattgc taatgatacc aaaaaaatta gtgccaatgc
     1081 actggatatt tatgttgcgg cacatttggg ttatgaattt gctttttggc gttttagtgc
     1141 cgcgcctctt ttgggcggtg gtttttcgtt tgcgagcatc aatctcgact acaacacgct
     1201 ttctacatta ccgcttacgc catcgggaga aaaacgcagc agcgctaaaa tcaacgccag
     1261 tgcgcctttc gccgaaggcg gcgtgaagct cgcgttccaa gttactaaaa atatttatct
     1321 acacgcgtac gcgctttacc ggcaaagttt ttatatgtcg acttcggtcg gcgaattatt
     1381 cacgttaggt ggattgagca tgcgtttcta aagcgtaagg agggtatatg aaaagtagga
     1441 tttacactgc gggcgtttt
//
LOCUS       DAOMPD010000612         1457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32261_length_1457_cov_2.631954, whole genome shotgun sequence.
ACCESSION   DAOMPD010000612 DAOMPD010000000
VERSION     DAOMPD010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32261_length_1457_cov_2.631954"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..>1457
                     /locus_tag="PLY93_14750"
     CDS             228..>1457
                     /locus_tag="PLY93_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRP70781.1"
                     /translation="MKTLLKIYLLFFLFLFVSHCVFHIAYLDSPASFMAYLVALRYDA
                     ATLSFLLGLPALLYLLAWRWVLLRKAVLWVIAFLSHYTFVVLFSDLFYYGLAGKHGTV
                     ELQIYFSNYIDTNLMAIREYPLMALGYVLSIVIFFLLWKRRIAVEGITIPHNFARQNL
                     LRKFSIVLLFIFVHIVAFRGGGQGRPLRPANAFEALTQAEGDFALNGVYTTSYALFHR
                     TSFPVRGSPESIQNARKFIQAKGDVFIEDSYPFLRRVIHPKPAQKKNVVFIILESWSG
                     KRVGIFGDTTNATPFFDTLARKGWLFTNAYATGRRSIASLPAAISSIPSLYGTLYITS
                     PFEQNFQRGMGAIFREAGYATHFTYAAKAGSMGFNTFARLAGFETILTRESFPADAPT
                     DGVWGVYDHVMFQRVLSD"
BASE COUNT          313 a          355 c          345 g          444 t
ORIGIN      
        1 attaaaaacc tcctctattt gtgcacgaaa attgatagtt ggcgcacagg ctcaacagag
       61 tctgtgcgcc aactcgcgca gcgtgcaaaa aaattatctc agtgagttgg cgctcaggct
      121 cctcattgaa atctatgcgt catgaagttc taaaaaagtg gtcagcctgt acgtgatttc
      181 cacacggagc ggagtaaatc tgtatccggg aaatttcact acctttcatg aaaacattgc
      241 tcaaaattta tcttcttttt tttctattcc tttttgtctc tcattgcgta ttccatattg
      301 cctatcttga ttcaccagcg tcgtttatgg cgtatctcgt ggcgttgcgc tacgacgcgg
      361 cgacgctttc ttttctactg ggactccctg cgctccttta cctattggcg tggcgctggg
      421 tgctgttgcg caaagccgtg ctgtgggtaa tcgcttttct ttcgcattat acgtttgtag
      481 tgcttttttc agaccttttt tactatggcc ttgcgggaaa gcacggcacg gtggagttgc
      541 agatttattt tagcaattac atcgacacta atttgatggc aatccgcgag tatccactca
      601 tggcgctggg atatgtcctt tcgattgtta tttttttcct actctggaaa cggcggattg
      661 cggtagaagg gataactatc cctcacaatt ttgcgaggca aaatttattg aggaaattta
      721 gcatcgttct cctctttatt tttgtgcata tcgttgcctt ccgagggggg gggcagggac
      781 ggccgttacg ccccgccaat gcctttgaag cgcttaccca agctgaaggg gattttgcct
      841 taaacggtgt ttataccact tcgtatgcac ttttccaccg tacctcattt cctgttcgag
      901 gttctccaga aagcatccaa aatgcccgta agtttattca agccaaggga gatgttttta
      961 tagaggatag ttatcccttc ttgcgtcggg ttatccatcc caagccagcg cagaaaaaaa
     1021 acgtcgtttt tattatttta gagtcgtggt cgggtaagcg ggtgggaatt ttcggcgata
     1081 caacgaatgc gactccgttt ttcgacactc tcgcacgcaa aggctggctt tttacgaatg
     1141 cgtacgctac cggccgccgc tcgattgcat cgttaccggc agccatcagc agcatccctt
     1201 cgctttatgg cacgctttac atcacttcgc cgtttgagca aaatttccag cgcggcatgg
     1261 gcgccatctt tcgtgaagca gggtatgcga cacacttcac ttacgctgcc aaagcgggct
     1321 cgatgggctt taatacgttt gcgcgtttgg cgggttttga aaccatttta acgcgcgaga
     1381 gtttccccgc cgatgcaccc accgacggtg tatggggcgt ttacgatcac gtcatgttcc
     1441 aacgagtgtt gtccgat
//
LOCUS       DAOMPD010000613         1454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32337_length_1454_cov_2.057898, whole genome shotgun sequence.
ACCESSION   DAOMPD010000613 DAOMPD010000000
VERSION     DAOMPD010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32337_length_1454_cov_2.057898"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1454
                     /locus_tag="PLY93_14755"
     CDS             <1..>1454
                     /locus_tag="PLY93_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HRP70782.1"
                     /translation="LENEDDLKLEAKNRPPEQVIVVADSVKQALRLASVEMQVDPLTL
                     DYKILNRGGKQLLGLRRIPYRIHVFKSKEDNRFSDLADMDISLSSAMAQAAFSEDTPI
                     AINAEGKILVRIYRTGVYITVRPPVGDGAPVSLQQALEKLRLAGVTAFDKGRLESELK
                     NKSGKQIKIAEWTPRPEADSQLSIEISADHLRATMMMTRPRPGGRHLQVSDVMQALKA
                     NGVQFGFKESTIEKALDEEMYSTPIVVAEGKTPQHGRDGYVDYKVRTERKIEFKEDES
                     GRIDFLDRDLVENVIQGQVLAVLVPAQRGQEGRDLFNKITPAKNGNPVTLKPGPGTML
                     SDDGKRLLAEKNGQVLYRGGILSVHDMFTVNGDVGISTGNITFLGSIVVTGNIDDNMH
                     VKAAGSIEVAGTIQKAFVEAEGDIIVRQGIIGRDNAQIESTAGSVYGKFLQNSTCMAE
                     LDIVMAENIMHSRVYAGRNVICNGKRAQLVGGEV"
BASE COUNT          417 a          328 c          386 g          323 t
ORIGIN      
        1 ttagagaacg aagacgatct gaagttagag gcaaaaaatc gccctcccga acaggtgatt
       61 gtcgttgccg attcggtgaa gcaagcgctc cgcttagcat cggtcgagat gcaagtcgac
      121 cccctgacgc tcgactataa aattctcaac cgaggcggga aacaactttt gggactacgt
      181 agaataccat accgaataca cgtctttaaa tcgaaagaag ataaccgctt tagcgatctc
      241 gcagatatgg atatttcact ctcaagcgcg atggcacaag ctgccttttc ggaagatacc
      301 cctattgcaa ttaacgccga gggaaaaatt ttggtgcgca tttaccgcac aggcgtttat
      361 ataacggtgc gtccaccggt gggcgacggt gcaccagttt cgctacaaca agcgttggag
      421 aaattacgtt tagccggagt gactgcgttt gataaaggcc gccttgaaag tgaacttaaa
      481 aacaagagcg gcaaacaaat taaaatcgcc gaatggacgc cgcgtcccga agcggattcg
      541 caactctcga ttgaaatttc agccgaccac ttgcgtgcaa cgatgatgat gacgcgcccc
      601 cgcccgggag gaaggcattt gcaagtgtcc gacgtgatgc aggcgcttaa agcaaatggt
      661 gtacagtttg gttttaaaga aagtacgatt gagaaagcgt tggacgaaga aatgtattcg
      721 acgcccatcg tggttgccga gggtaaaact ccgcaacacg gtcgcgacgg ctacgtcgat
      781 tataaggtgc gcaccgaaag aaaaattgaa ttcaaagaag acgagtcggg tcgtattgac
      841 tttttagacc gtgacttagt tgaaaacgta attcaaggac aagtgcttgc agtcctggtg
      901 cctgcacagc gcggtcaaga agggcgcgat ttatttaata aaatcactcc cgcaaaaaac
      961 ggaaacccgg taacgctaaa accaggacca ggcaccatgc tttccgacga cggcaagcga
     1021 ctcttagcag aaaaaaatgg tcaagtgttg tatcgtggcg gtattttgag cgtacacgat
     1081 atgtttaccg tgaacggcga tgtaggtatc agcaccggta acattacctt cttaggttct
     1141 atcgttgtga ccggcaacat cgacgataat atgcacgtca aagcggcggg aagcatcgaa
     1201 gttgccggta cgattcaaaa ggccttcgtc gaagccgaag gagacatcat cgtgcggcaa
     1261 ggtatcatcg gtcgcgacaa tgcacaaatc gaatcgacgg cgggctcggt gtacggtaag
     1321 tttttacaaa actcaacgtg tatggccgag ttggacattg tcatggccga gaatattatg
     1381 cactcgcgtg tttacgcagg acgcaatgtt atctgtaacg gtaaaagggc gcagttggtc
     1441 ggtggcgaag ttat
//
LOCUS       DAOMPD010000614         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32403_length_1452_cov_2.189692, whole genome shotgun sequence.
ACCESSION   DAOMPD010000614 DAOMPD010000000
VERSION     DAOMPD010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32403_length_1452_cov_2.189692"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLY93_14760"
     CDS             complement(<1..366)
                     /locus_tag="PLY93_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRP70783.1"
                     /translation="MEKRSLKTLNTFGIDVLANCIEFFQSEEELTTLLQQENIRKQFM
                     ILGGGSNILFCHDFDGAILKNEIKTLNFTDKGAITTATVGGGFTWHSFVETCVQKSLY
                     GVENLALIPGTVGASPIQNI"
     assembly_gap    742..758
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <759..>1452
                     /locus_tag="PLY93_14765"
     CDS             <759..>1452
                     /locus_tag="PLY93_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRP70784.1"
                     /translation="VAAGLFPSAVAGFVLNQGEKETLRVVSGHAVHYDWTYANAGNPE
                     FKSLYRRAYRGQWNAEDLPWQTDVDPFNPNIAIIPERLVPGFGEKFYPTDLRTRQQVQ
                     HSAISFLLSQFLHSEQGALFIAGETVEASPWFDAKLYGSTQVVDEGRHVEVFSRYLQE
                     KLEKLYHVNDNLFVILRAITADSDWDLKFLGMQIMIEGLALGAFSTLYKFTEEPLLKQ
                     LLKQVISDEARHV"
BASE COUNT          359 a          298 c          343 g          435 t
ORIGIN      
        1 aatgttttgg atgggcgaag ctcccactgt gccggggatg agcgctaagt tctccacgcc
       61 gtagagtgat ttttgtacgc aggtttcgac aaagctgtgc caagtaaatc ctccacctac
      121 cgtagcggtt gttattgcac ctttatccgt aaaattgagc gttttaattt cgttcttgag
      181 aattgcgccg tcaaaatcgt ggcagaatag tatattggaa cctcctccta agatcataaa
      241 ttgtttgcgg atgttttctt gttggaggag tgtggtgagt tcttcttcgc tttgaaaaaa
      301 ttcaatacag tttgcgagta catctatacc aaaagtattg agtgtcttca acgaccgctt
      361 ttccataggg ggtctggtct tattttattt tttagacgtt gcggcgaaag gaatacggag
      421 ttaatgaatt taattttcgg tagtgtcccg tcaattcggt gtacacttcc ttcttgagtt
      481 ttagaaggtc gtgtaattcg taaatcaaat tagtgaattt ttgttcaaga ttatgattat
      541 ccttaatgtg acataattca ggatagttac cccttattgt gacataatgc atagaagtct
      601 atagccagac aaaaactatt tacagagcga tagttttttt gtgtatggtt gtttattggc
      661 ttggcgctca cgctgaggca atattattta aaggagtaat ataatactat ggaaatttta
      721 gaagatgttt caactttagg cnnnnnnnnn nnnnnnnngt tgcagcagga cttttccctt
      781 cggctgtggc gggttttgtc ttgaaccaag gcgagaagga aacgctgcgt gtggtctcgg
      841 gtcatgcggt gcattatgac tggacgtacg cgaatgcggg caatcccgaa tttaagagcc
      901 tttatcgccg cgcgtaccga ggccagtgga acgccgaaga tttaccgtgg caaaccgacg
      961 tagacccttt taatcccaat atcgccatca tccccgaacg tttggtgccg ggctttggcg
     1021 aaaaatttta tcccaccgat ttacgtaccc gtcaacaagt ccagcattcc gctattagct
     1081 ttttactttc gcaatttttg cacagcgagc agggcgcact ttttatcgct ggtgaaaccg
     1141 ttgaggcaag cccgtggttt gacgccaaac tctacggctc gacacaggtg gtggatgaag
     1201 gccgccacgt ggaagttttt tcgcggtatt tacaagaaaa gctcgaaaag ttgtaccacg
     1261 tcaacgataa cctttttgtg attttacgcg cgattaccgc agactccgac tgggacctca
     1321 aattcttggg gatgcagatt atgattgagg gcttggcgtt gggagcgttt agcactctct
     1381 acaagtttac cgaagaaccg ttgctcaaac aactcttaaa acaagtgatt tccgacgagg
     1441 cacgccacgt cc
//
LOCUS       DAOMPD010000615         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32432_length_1451_cov_2.948424, whole genome shotgun sequence.
ACCESSION   DAOMPD010000615 DAOMPD010000000
VERSION     DAOMPD010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32432_length_1451_cov_2.948424"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLY93_14770"
     CDS             complement(<1..223)
                     /locus_tag="PLY93_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRP70785.1"
                     /translation="MNGQTDATDTKNIFENVWDSLKAGFTALTLKLPDLYMDSIFLTR
                     IRAMLGGQLRGTISGGGALPLHIDEFYNAI"
     gene            complement(239..1156)
                     /locus_tag="PLY93_14775"
     CDS             complement(239..1156)
                     /locus_tag="PLY93_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP70786.1"
                     /translation="MRHLPDVFHFAATNFPNQGFYSKDTHKFFKSQPFRALHERASQF
                     AIHLIHEGLKPKENVALFGDNSAEWLIVDMAIQMANAVVVPRGSDSTPQELEFILEHS
                     EAKIVFIEHLRLYKKIAPLLKEKKLCVYVIDAAFPQKIADEDKITILPQFFKNLKPLS
                     AAEIKQLDTLRAKLTSSDLFTIIYTSGTTGEPKGVMLTHANMLYQIEVVPTALSMSPK
                     DRILSILPVWHIFERFMLYSVISSGAHYYYSNKKDLMEDFIRIKPTLMASAPRLWEQI
                     YQKLREQIDKTEAFNRELFDLSYGIKQRL"
BASE COUNT          424 a          282 c          323 g          422 t
ORIGIN      
        1 caatcgcgtt gtaaaattcg tcgatgtgaa gcggtaacgc accgccaccc gaaatggtac
       61 cgcgcaactg tccccctaac atcgcgcgga tgcgggtaag aaaaattgaa tccatgtata
      121 aatcgggaag ctttaatgtc aacgcagtaa atcccgcttt gagagaatcc catacattct
      181 caaaaatatt tttagtgtcc gttgcatcgg tttggccatt cactaaattc agcgcgcgct
      241 aaaggcgttg tttgatgcca tacgacaaat caaaaagttc gcggttaaac gcctcagtct
      301 tgtcgatttg ttcgcgcagc ttttggtaaa tttgctccca caaacgcggc gccgacgcca
      361 tgagagtggg tttaatacgg ataaaatctt ccattaaatc ttttttattc gagtagtaat
      421 aatgcgcacc ggaggaaatc acactataaa gcatgaaacg ttcaaaaata tgccacaccg
      481 gtaaaataga aagtatgcgg tctttgggcg acatcgaaag cgcggtcggt acaacctcga
      541 tttggtagag catgttggcg tgcgtgagca tcaccccttt gggttcgcca gttgttcccg
      601 aggtataaat aatggtgaat aagtcggatg acgtaagctt tgcgcgtaaa gtatcgagtt
      661 gttttatttc cgctgcgctg agtggcttta agtttttaaa aaattgaggt aagattgtaa
      721 tcttgtcctc atcggcaatt ttttgtggga acgctgcatc tatcacatag acgcatagtt
      781 ttttttcttt gaggagcggt gcaatttttt tatagagacg taaatgttcg ataaaaacaa
      841 ttttggcttc agagtgttct aaaataaatt cgagttcctg cggcgttgaa tccgatccgc
      901 gcggcaccac gacggcgttc gccatttgga tagccatatc cacgatgagc cattcggcag
      961 agttgtcgcc aaagagcgcg acattttctt tcggttttaa accctcgtgg attaaatgga
     1021 tggcaaactg cgacgcccgc tcgtgcaacg cacgaaaagg ttgtgactta aaaaacttat
     1081 gcgtgtcttt ggaataaaaa ccctggttgg ggaaattcgt cgcagcaaaa tggaatacat
     1141 cgggcaaatg gcgcattatt ctaaaagaat ttcaacgata gagcgtcgat tgaaaagatg
     1201 aacgtactgt caaaaaaagt gtatagagtg tttactcaaa tttgtgtact ataatctatt
     1261 gtgtaaggat agaaaggcta ttatcaacag cgttgagttt agattatgtc acatcgggga
     1321 taactatctc catctcatta tgtcacatta gaggataact atctaaaccg ataatttttt
     1381 ctctaaaaat tacacttttt tttcagaaaa atgagaaaat ttgactgttt tctggtattt
     1441 ataatataga a
//
LOCUS       DAOMPD010000616         1446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32572_length_1446_cov_2.450755, whole genome shotgun sequence.
ACCESSION   DAOMPD010000616 DAOMPD010000000
VERSION     DAOMPD010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32572_length_1446_cov_2.450755"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..592
                     /gene="coaD"
                     /locus_tag="PLY93_14780"
     CDS             98..592
                     /gene="coaD"
                     /locus_tag="PLY93_14780"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRP70787.1"
                     /translation="MRHAVYPGSFDPFTNGHLDIVQRSIKIVDTLTIAVLRNTSKKSM
                     FTPDERVEIIRAVVKEYRQVKVETFEGLLVDFCRTHGIRTVIRGLRAVSDFDYEHAIF
                     LMNRKLLPDLETMFFMAGSENSFISSTIVKEVAHFGGNIADQVPPLVLERMLKKKNSG
                     AENV"
     gene            585..>1001
                     /locus_tag="PLY93_14785"
     CDS             585..>1001
                     /locus_tag="PLY93_14785"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70788.1"
                     /translation="MFSFRKAIGLVALSAILIALSFVPYNLYLLGFIGFLPLFFVFEN
                     FTPNKKQIFFLWLVWVLILNFIAYHWIMHTIAVYGMMPTAVAFPLFLLYTLGTSGKML
                     LFFIFLNHIQKKSPLFSGTYTTLFAIVVAFGLAELVG"
     assembly_gap    1002..1017
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1018..>1446
                     /locus_tag="PLY93_14790"
     CDS             <1018..>1446
                     /locus_tag="PLY93_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70789.1"
                     /translation="PWYGGNIVSGDLYFAQAADIIGTRGLSLVWTLLQYLFYLTLKEA
                     IRLKKEKAWKAIGLFRPLWISIAILALTHSYGAIRYHQAAAKENSATKVRVGVPQGNV
                     PLITYRHERPYIISRMLAQTENLVQEAAAAQKPLDLVVWP"
BASE COUNT          352 a          358 c          321 g          399 t
ORIGIN      
        1 aatacccccc aattgcggcg tagcatttgt caggtatgtc cgtgggttag acattttgaa
       61 ctactttaca caatcacacc aaaatcaaaa ctaccgtgtg cggcatgcag tttatccggg
      121 aagtttcgac cccttcacca atggccatct cgatattgtg caacgttcga taaaaattgt
      181 cgacacgctc accatcgccg tcctcagaaa tacttccaaa aaaagtatgt tcacccccga
      241 cgagcgcgtt gagatcatcc gcgcagtggt gaaagaatac cggcaagtca aagtagaaac
      301 gtttgaaggg cttttagtcg atttttgccg cacgcatggc attcggacag ttatccgtgg
      361 cttgcgtgcg gtgtccgatt ttgattatga acacgccatt tttttgatga accgtaaact
      421 attgcccgat ttagaaacca tgtttttcat ggcgggctct gaaaactcgt ttatttcgag
      481 cactatcgtc aaagaggtgg cacacttcgg cgggaacatc gccgatcaag tgccgccgtt
      541 ggtgctcgaa cgtatgctca agaaaaaaaa tagcggcgcc gagaatgttt agttttagga
      601 aagcgatagg cctcgttgcg ctctccgcca ttctcattgc gctctcgttt gtgccgtaca
      661 atctttacct attgggattt ataggttttt tgcctttgtt ttttgtcttt gaaaatttta
      721 ctcccaacaa aaaacaaatt tttttcttgt ggctcgtgtg ggtgctcatt ctcaacttta
      781 ttgcatacca ctggataatg cacaccatcg ccgtctatgg catgatgccc actgcggtgg
      841 cgttcccact ttttttatta tataccctcg gcaccagtgg caagatgctc ttgtttttca
      901 tcttcctcaa ccatatccaa aaaaaatctc ctttgtttag tgggacttac actactctct
      961 ttgccatcgt cgtagcgttt ggattagccg agcttgtagg cnnnnnnnnn nnnnnnntcc
     1021 gtggtatggc ggcaatatcg tttcggggga tttatacttt gcgcaagcgg cagacatcat
     1081 cggcacgcgt ggcctatcgc tcgtgtggac gctcttacaa tacctctttt atttaacact
     1141 caaagaagca atccggctta aaaaagaaaa agcgtggaag gcgataggcc tttttcgccc
     1201 gctttggatt tccatagcta tcctcgcgct aacgcacagc tacggcgcaa tacgctacca
     1261 ccaagccgcc gcgaaagaaa atagtgcgac taaagtgcgt gtaggtgtgc cgcaggggaa
     1321 tgtaccgctg atcacttacc gccacgaacg cccctatatt atctcacgca tgctcgcgca
     1381 gacagaaaat ttagtgcaag aagctgcggc ggcacaaaaa ccgctcgatt tggtcgtctg
     1441 gccaga
//
LOCUS       DAOMPD010000617         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32600_length_1445_cov_2.277698, whole genome shotgun sequence.
ACCESSION   DAOMPD010000617 DAOMPD010000000
VERSION     DAOMPD010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32600_length_1445_cov_2.277698"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1022)
                     /locus_tag="PLY93_14795"
     CDS             complement(84..1022)
                     /locus_tag="PLY93_14795"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HRP70790.1"
                     /translation="MKIQQIALVFMATLIAHCAQINKEKIAGETPATTSPNSGDGGTS
                     PFNAQLATGVVDAPCNGHATDGCQPSFSDPIKATNGVKGAGWGSGSFDVYSMSESTAD
                     GGKYATLTLDWQGATVKNVQGVDFVVYENAFEDLGTYGTRFMDLIVVSVSQNNTDYCG
                     WPVEYKGKKGNDSLADFQNGSLTEDKTLYSKWPEAWVHFAGKSPTAWNQDTAPMSYDD
                     LFTTKTTEYFNGQTLMYSRTDLKGGGDGFDLDALVTNASCSAAVRDAIQADGFKYIKL
                     QAAHSLINPDTGSLYPHEAISNGPDVDGILARHVVP"
     regulatory      complement(1095..1266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          316 a          388 c          352 g          389 t
ORIGIN      
        1 gattttcctc agcgtggatc acgttccata gtcccaacac taccgcaacg caacatttcg
       61 cgttacgctg gctaaaaaaa tttttatgga acaacgtgcc gtgccaaaat accgtcgaca
      121 tcggggccgt tcgatatcgc ctcatgtgga tagagagaac cggtatcagg gttgataaga
      181 ctatgcgctg cttggagctt tatatactta aaaccatctg cctggatggc gtcgcgcacc
      241 gcagcgctac aagaagcgtt tgtgaccaaa gcatctaaat cgaacccgtc accgccgcct
      301 ttaagatcgg tacgtgaata catgagcgtc tgcccattaa agtactcggt ggttttggtc
      361 gtgaatagat cgtcgtagct catgggggcg gtgtcttgat tccacgccgt cggggatttt
      421 cccgcaaagt gtacccacgc ttctggccac ttggaatata aagttttatc ttccgttaaa
      481 ctcccgtttt ggaaatcggc caaactatcg tttccttttt tgcctttata ttccacaggc
      541 catccgcaat agtcggtgtt gttttgactc accgacacga cgattaaatc cataaaccgt
      601 gtaccgtatg tacccaaatc ctcaaaggca ttttcataca cgacgaaatc caccccttga
      661 acatttttca ccgtcgcgcc ttgccagtcg agagtgagcg ttgcgtattt accaccgtcg
      721 gcggtgcttt cagacattga gtacacgtcg aatgaacccg agccccagcc tgcgcctttg
      781 acgccgtttg tcgctttgat tggatcgcta aacgatggtt ggcagccgtc ggtagcgtgg
      841 ccgttacacg gagcgtcgac gacccccgtt gcaagttgtg cgttaaaggg agatgttcca
      901 ccatccccgc tgtttgggct ggttgtggca ggtgtttcac ctgcaatttt ttctttattg
      961 atttgcgcac aatgcgcgat gagcgttgcc ataaagacaa gcgcaatttg ttgtatcttc
     1021 atacagcccc cttaggagcc cgcttgccgc cgaaatggaa aatatttttc gagcagcgtt
     1081 tcgccatggc acccgtgtcc tcggagtgct tgagcaagag ttccagactt agattcgggt
     1141 gaacataggg gacgcccttg cgtttcctat gttcacgctt tcattactgt tgcgggtcag
     1201 cgccggaatt gcaccggact tcctctttgt gagtggcttg agccaaccca cactacaaac
     1261 tatcgtccag agacggtgcg tcaactggaa gagctctcct caaacccgct cgaaaattgg
     1321 cgtcagctta tcttcgtatg ccttgaagcg caccttgtcg ccgatttttg ccgtgttggc
     1381 gtcgggcatt tcgacaatcg ccgtcatgcg catcttgtcg tcggtctcga caaccgcgag
     1441 gcaat
//
LOCUS       DAOMPD010000618         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32695_length_1442_cov_1.927181, whole genome shotgun sequence.
ACCESSION   DAOMPD010000618 DAOMPD010000000
VERSION     DAOMPD010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32695_length_1442_cov_1.927181"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLY93_14800"
     CDS             <1..823
                     /locus_tag="PLY93_14800"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRP70791.1"
                     /translation="GARVHTARSGFTIDVAFSFLSNLTKSAKVLMAGGQQVGYVQSIF
                     QKDYQTYVRLYLDNRLKNKIPNTKETQIAIFSNNLMGQKYINLQFEPLKPGDAILHAG
                     EIIRGISPPSFEQMMLSFSSWFEGKSAGEVAEQIFAKAALLQRDIDSVFNENREDLEA
                     TVNGAKNYFTNFSSQFDAFKTNITSIAHNSEDILTAQQQSLTELIQNTSTIAKNLERL
                     ELALRSNKGTLGKFSNESKALRENLRLTSEYSRSFLKCIQERPWVIIYKESCRKR"
     gene            complement(824..>1442)
                     /locus_tag="PLY93_14805"
     CDS             complement(824..>1442)
                     /locus_tag="PLY93_14805"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRP70792.1"
                     /translation="LTQIAWQHDVQTMIEGPGHVPMHLIQENMDLQLKICHEAPFYTL
                     GPLVTDIAPGYDHITSAIGAAMIGSMGTSMLCYVTPKEHLGLPDKDDVKTGVITYKIA
                     AHAADLAKNHPGARDRDNALSKARFEFRWEDQFNLSLDPETAQKYHDATLPQSGMKKA
                     HFCSMCGPQFCAMKLTHEVREIADATKGMEDKAKEFKAKGGEIYV"
BASE COUNT          382 a          366 c          330 g          364 t
ORIGIN      
        1 cggtgcacgc gtccacaccg cccgctcggg ttttaccatc gatgtggcat tttctttttt
       61 aagcaacctc accaaaagcg caaaagtact catggcgggt ggccaacaag tgggttacgt
      121 gcagagtatt tttcaaaaag actatcaaac atacgtgcgt ctttatttag acaaccgcct
      181 caaaaataaa atccccaata caaaagaaac tcaaatcgcc atttttagca acaacctcat
      241 ggggcaaaag tatatcaatt tacaatttga accgttaaag cctggagacg cgatcctaca
      301 cgcaggtgaa atcattcgtg ggatatctcc cccttcgttc gaacaaatga tgctttcttt
      361 ttcgagttgg ttcgaaggaa aaagcgcagg cgaagttgcc gaacaaattt ttgccaaagc
      421 cgccctctta caacgcgaca tagactctgt ctttaacgaa aaccgcgaag atttagaagc
      481 caccgtgaac ggagctaaaa attattttac gaatttttca tcgcaattcg atgcgttcaa
      541 aacgaacatc acctcgattg cccacaacag cgaagatatt ttaaccgcac aacaacaaag
      601 cctgaccgag ctcatacaaa acacatcgac gattgcaaaa aatttagaac gccttgaact
      661 ggcgttgcgt tccaacaaag gcacgcttgg caaattcagc aacgagagca aagccctccg
      721 cgagaatcta cgtctaacat cggaatatag tcgctctttt ttgaagtgca tccaagaacg
      781 gccttgggtt attatttaca aagagtcctg ccgcaagcgg taactacacg taaatttcgc
      841 cacctttcgc cttaaactct ttcgctttgt cttccatgcc tttggtggcg tcggcaatct
      901 cgcgcacttc gtgcgttaat ttcatcgcac aaaattgggg cccgcacatc gaacaaaagt
      961 gcgctttttt catccctgac tgtggcagag tcgcgtcgtg gtatttttgc gcggtctcgg
     1021 gatcgagcga aagattgaat tggtcctccc aacggaactc gaagcgtgct ttagaaagtg
     1081 cattgtcgcg atcgcgcgca cccgggtggt ttttcgccaa gtcggcggca tgcgcggcga
     1141 ttttatacgt gatgactccg gttttcacgt cgtctttgtc gggaaggccc aagtgttctt
     1201 tgggcgttac ataacagagc atcgacgtgc ccatcgaacc aatcatcgcc gcgccgatgg
     1261 cggaggtaat gtggtcgtat cccggggcaa tgtcggtgac gagaggcccc agtgtataaa
     1321 acggcgcttc gtggcaaatt ttaagttgca aatccatatt ttcttggatg aggtgcatcg
     1381 gcacatgccc cggaccttcg atcatcgttt gcacgtcgtg ctgccacgca atttgggtga
     1441 gc
//
LOCUS       DAOMPD010000619         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_32805_length_1438_cov_2.339841, whole genome shotgun sequence.
ACCESSION   DAOMPD010000619 DAOMPD010000000
VERSION     DAOMPD010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32805_length_1438_cov_2.339841"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PLY93_14810"
     CDS             <1..989
                     /locus_tag="PLY93_14810"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HRP70793.1"
                     /translation="YFTTGQSALAALESNTYDLAFLDIYLPDISGLAVLDAMRLSKKH
                     SGIPILAMTADVTEEMSVTALMKEADEVLLKPLRHTEIVIKAKLWLERQAKRIKLHET
                     NIRLQAQSKIMASYFPDDVIQKLTDTGAMMVAGVIPTTVMFFDLHNFTALSEKIEPAQ
                     VAELLNSIFTDLMDLIFAHHGSVNKFIGDAIMATFGAPLSQGNDALNAVRCAFTIKES
                     MALFNQVRPPYLTQELNFGIGIATGSVFAGTVGSSRRLEYTILGDTVDVASRLESLNK
                     ISKTKILIDGATYEKTKHYLNVRRLCFQKKIRGKSHEFEVFSPHEILNEPSP"
     gene            1004..>1438
                     /locus_tag="PLY93_14815"
     CDS             1004..>1438
                     /locus_tag="PLY93_14815"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRP70794.1"
                     /translation="MKKKLIRIDSFSALSPSEKHALFTRKTLDLTQVKNDVIFPMAEA
                     FRKNPMSALKEATLKYDRFCPEPLILTEKKIKAAYDRTAKIDPTTLEAFRKAAANITA
                     FHEKQIQTGFETQIAGNTLGMRFIPFDRAALYVPGGKALYPST"
BASE COUNT          427 a          377 c          288 g          346 t
ORIGIN      
        1 attattttac caccggtcaa agtgcccttg ccgcactcga aagcaatact tatgacttag
       61 cctttttaga catctatttg cccgacattt ctggcctcgc tgtgctcgac gcaatgcgtt
      121 taagcaagaa gcactccggc atccccattt tggcaatgac cgccgacgtc accgaagaga
      181 tgtcggtgac tgcactcatg aaagaagccg atgaagtatt gctaaaaccc ttgcgccata
      241 cagaaatcgt tatcaaagcc aagctctggt tagagagaca agccaagcgc ataaaattac
      301 acgaaaccaa catccgatta caggcgcaat cgaagattat ggcaagctat ttcccggacg
      361 acgtcattca aaaactgacg gacactggcg ccatgatggt ggcgggggtt atacccacca
      421 cagtgatgtt ttttgattta cacaatttta ccgcactttc agaaaaaatt gaaccagctc
      481 aagtcgccga actcctcaac agtattttta ccgacctcat ggatttaatc ttcgcacacc
      541 atggctccgt aaataaattt atcggcgatg cgattatggc cacctttggc gctccacttt
      601 cgcagggaaa cgatgcgtta aacgccgtgc gctgtgcatt taccatcaaa gaatcgatgg
      661 cgcttttcaa ccaagtgcgc cccccctact taacccaaga actcaatttt ggaatcggta
      721 tcgccactgg ttcagtattt gcaggcactg ttgggtcgtc tcgtcgatta gaatacacca
      781 tcttgggcga cactgtcgac gtcgcaagcc gtttagagtc gctcaataaa atatcgaaaa
      841 caaaaattct catcgatgga gcgacatacg aaaagacaaa acactatctc aacgtgcgcc
      901 gtttatgttt tcagaaaaaa attcgaggca aatcgcatga atttgaagtt ttttcacccc
      961 atgaaatttt aaacgagcca tccccataat tgtagcccta agcatgaaaa agaaacttat
     1021 tcgtatcgat tccttctctg cgttaagccc ctctgaaaag cacgcgctct ttacacgaaa
     1081 gacactcgac ttaacccaag taaaaaacga tgttattttc cccatggctg aagcgttccg
     1141 caaaaatccg atgtccgcgc ttaaagaagc cacactcaaa tatgatcgct tttgtcccga
     1201 accgttgata cttacagaaa aaaaaattaa ggcggcgtac gatcgaaccg caaaaatcga
     1261 ccccaccacc ctcgaagcgt tccgtaaagc ggcggcgaac atcactgcgt ttcatgaaaa
     1321 acaaatccaa acggggtttg aaacccaaat cgcaggtaac acactaggaa tgcgctttat
     1381 tcctttcgat agagccgcac tctacgtgcc agggggcaag gcgctctatc catcgacg
//
LOCUS       DAOMPD010000620         1425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33172_length_1425_cov_3.757664, whole genome shotgun sequence.
ACCESSION   DAOMPD010000620 DAOMPD010000000
VERSION     DAOMPD010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33172_length_1425_cov_3.757664"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..>1425)
                     /locus_tag="PLY93_14820"
     CDS             complement(163..>1425)
                     /locus_tag="PLY93_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70795.1"
                     /translation="IAYTIFDPTTGAEKYPSLALEEFKASLKADDFQEEKMFTSTILD
                     FSSNTAPIASDADLTTRRLVMENQKNPQPLSEVLLPELAPISEKQNFTFDDITKYVLN
                     PFEYFYKPLTHQSENLLEFNYNTPRLAESSSSRYRFIERYIYDALMNPQEELSLEPLQ
                     KLAGEERCAKLPPEGFDFMRRFLEKKDEASSQTYNAEIFTRAKLARQFYQIHFFIFDE
                     RITRPLRIPETERFIRIYLPAPRVGRVIITGQSFPLLYNTSDNEYFYLSPYEERSTSA
                     VKLYLLLALIKQTKLEAFTLDSIKKREYEFKEKDNSISEKIQFTLTLNKLQQIVERHL
                     EEILTQLQNQNATCFSIDYLGGNNIERYNDKDDHGIETLFQTEYEKVNSIAHFFQKKP
                     SSASVDFYKTIIRPMAECILGNKMKKKN"
BASE COUNT          361 a          246 c          319 g          499 t
ORIGIN      
        1 gttgtttttc ccgagcctgc cgacgcctca ataaaaagat gggtctcttt ttggataagc
       61 tccctcactt tttctttttc aacggttgcg gatggttcaa taatggtctt ctttgaaggc
      121 ttgttgtctt ttggggtggg agtaggagtt ttcttttgca tatcagtttt ttttcttcat
      181 tttattgcct aaaatacatt cggccatggg gcgaatgatg gttttgtaaa aatcaacgct
      241 tgccgagctg ggcttctttt gaaaaaaatg ggcaatcgaa tttactttct catattcggt
      301 ttgaaacaat gtttcaatac catggtcgtc tttatcatta tatctctcaa tattatttcc
      361 cccaaggtaa tcgatgctaa agcaggtggc gttttggttt tgtaattgcg tgagaatttc
      421 ttccaggtgg cgttcgacta tttgttggag cttattgagc gtcagcgtga attgtatttt
      481 ttcagaaata ctattatctt tttctttaaa ttcatattct ctttttttga tagaatccaa
      541 tgtgaatgct tcaagtttgg tttgttttat taacgccaga agaagataga gctttacagc
      601 gctcgtggag cgttcttcgt aaggcgataa gtaaaaatat tcattatcac ttgtattgta
      661 gagtaaggga aagctctggc cggtgatgat cactcttcca acgcgtggcg cgggtaaata
      721 aatacggata aacctctctg tctccggaat gcgcaggggg cgggtgatac gttcgtcgaa
      781 aataaaaaaa tgtatttggt agaattgtct cgcaagtttt gcacgggtga agatttctgc
      841 gttataagtt tggctggatg cctcgtcttt tttctctaaa aaccgccgca tgaaatcaaa
      901 gccttcggga ggaagttttg cacacctttc ttcccctgct aatttttgga gcggttctaa
      961 agataattct tcttgtggat tcatcaacgc gtcataaata tatctttcaa taaaacgata
     1021 ccgcgaactg ctcgattccg ctaagcgagg tgtgttgtaa ttaaattcca ataaattttc
     1081 gctttggtgc gtgagtggtt tatagaaata ctcaaaagga tttaagacat attttgtaat
     1141 atcatcgaaa gtaaaatttt gtttttctga aataggcgca agttcgggta ataaaacttc
     1201 agaaagaggt tgtggattct tttgattctc cataactaat cgccgggtgg ttaaatctgc
     1261 gtcggacgcg attggcgctg tgttggacga gaaatctaaa attgtcgatg tgaacatttt
     1321 ttcttcttga aaatcgtctg ctttgagcga cgccttaaat tcttcgaggg caaggcttgg
     1381 gtatttttca gcgccggtgg taggatcaaa aattgtgtat gcgat
//
LOCUS       DAOMPD010000621         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33245_length_1423_cov_2.472222, whole genome shotgun sequence.
ACCESSION   DAOMPD010000621 DAOMPD010000000
VERSION     DAOMPD010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33245_length_1423_cov_2.472222"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1423
                     /locus_tag="PLY93_14825"
     CDS             <1..>1423
                     /locus_tag="PLY93_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70796.1"
                     /translation="VTYSDGPPTIQSALALDTNYNGKIDTYQVTFNKNVNDSTFPGYI
                     ANAVGGAQTQWLVSGYTNVRLLHGTAVTFTADTANDAVLYIRFNENVLLCDTTDTVGC
                     DTDVKPDLTTTATPGVKDMGGQTLAQVGVATVTEADGAAPVLVRAFSLLPTSVDTIFS
                     EPMTAMQVNVWSNYTITGGSGLAVSAAAIDGINKRIVHLTTSAQTGGTVYQLTASTVL
                     RDLTGINLITNTNPTTTLPANQADFTGVEKPVVVSIATKSSTTLLLTINESIVESSAI
                     CSNLVACSSVYENLSLPVLSAQAADASGIPTAGDSKYFLLKVNPMIEGQAYTTKVLQN
                     TMTSTATGLKMGSANNSATFNGDGNPAATIDVSTATVCPAAGADQYKKVIVQYDQAVG
                     ASALTTSNYKITGCLSGTCTSGHTGANTSGAAAVAFLGGNKYEIKFTNQILPTTANFQ
                     LTISGVQDTNGNTIAAPGNSSFQ"
BASE COUNT          385 a          365 c          358 g          315 t
ORIGIN      
        1 gcgtaacgta tagcgatggt ccgcccacaa tacaaagcgc tcttgcactc gacacaaact
       61 acaacggcaa aattgatacg taccaagtga cgttcaacaa gaatgtgaac gatagtactt
      121 tcccaggtta catagccaac gcggtggggg gcgcgcaaac tcaatggctt gtgtcggggt
      181 acacgaatgt gcgtcttctc cacggcacag cggttacttt taccgccgac accgcaaacg
      241 atgcggtcct ttatatccgc tttaatgaaa acgttttgct ttgcgatacc acggacaccg
      301 tcggttgcga caccgatgtg aaacccgatt taaccacaac ggcaacgccg ggggtcaaag
      361 atatgggagg ccagacactg gcacaagtgg gcgttgccac tgtcaccgaa gccgacggcg
      421 cagcgccggt gttggtgcgg gcattttcac tcctcccaac gtcggtggat accattttta
      481 gcgaacccat gactgcaatg caagtgaatg tatggagtaa ctacaccatc actggtggca
      541 gcggactcgc cgtgagcgct gcagcgattg acggcattaa taagcgcatt gtgcatttga
      601 cgacaagcgc acaaactggc ggtacggtat accaactcac agcatcgact gtgttgcgtg
      661 accttacagg gataaatctc attacaaata cgaaccccac aacgacgctg cctgcaaacc
      721 aagcggactt caccggtgta gaaaaaccgg tggtggtgag tattgccacc aagtcttcga
      781 caacgctctt actcacgatt aacgaatcga ttgttgaatc gagcgctatt tgttcaaatt
      841 tagtggcatg ttcttccgtt tatgaaaact tgtcgcttcc agtgttgagt gcccaagcgg
      901 cggatgcatc ggggattcct accgcagggg attcaaaata ctttcttttg aaagtcaacc
      961 cgatgatcga agggcaagcg tacacgacaa aggttttgca aaacaccatg acctcgacgg
     1021 ccaccggatt aaaaatggga agcgcgaata attcggccac gtttaatggc gatggtaatc
     1081 ccgcagcgac aatcgatgtt tccacggcga cggtttgccc tgctgcggga gccgaccaat
     1141 ataaaaaagt tatcgtgcag tacgatcaag cagtgggagc aagtgcgctc actacatcga
     1201 attataaaat caccggttgc ctttcgggta cttgcacgag cggacacacc ggcgcaaata
     1261 cgagcggtgc agcggcggtt gcttttttag gagggaataa atacgaaatt aaatttacca
     1321 atcaaattct ccctacgacc gcgaatttcc aactcacgat tagcggcgtg caagatacaa
     1381 acggcaacac cattgctgct ccagggaatt cgtcgttcca gtg
//
LOCUS       DAOMPD010000622         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33261_length_1423_cov_1.835526, whole genome shotgun sequence.
ACCESSION   DAOMPD010000622 DAOMPD010000000
VERSION     DAOMPD010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33261_length_1423_cov_1.835526"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1423
                     /gene="topA"
                     /locus_tag="PLY93_14830"
     CDS             114..>1423
                     /gene="topA"
                     /locus_tag="PLY93_14830"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRP70797.1"
                     /translation="MNHKIHRIEFNEITKDAVIHAVEHPRDIDMERVNSQQARRILDR
                     LVGYEISPVLQKKFGSRRFSAGRVQSAALKILCDREKEIEAFVPQEYWEADGFFATPD
                     AKAKDKETAFRLAQIDGKKTEIYNRTEAETAEKKIRSAKYTLLTRKTSERRIKPLAPY
                     ITSRLQQDASTRLGFRAQKTMSIAQNLYEGVDLGKEGTQGLITYMRTDSTRISQTGIE
                     QARAFISANFHAEHLPESANQYSKKNENTQDAHEAIRPTDVNRTHDSLEKYLDRDQFR
                     LYALIWRRFVASQMTPGVDELVTLEAEGKTGDAAETPQQFLFRYSSSRQLFDGFRAVY
                     PLGDEKAGYVPHFNEGDALTLIDFQKQQKFTQPPPRYTEASLIKSMEESGIGRPATYV
                     PTMATLDKRAYIERKGKQLIPTKLGRVVNDIMAAHFPDIVNLQFT"
BASE COUNT          402 a          406 c          342 g          273 t
ORIGIN      
        1 cattgggaga gctgcgcaag gcggctaaaa attcatccga ggtattactc gccagcgacg
       61 ccgaccgtga agggtaagcg attagttggc acttaggccg cgcgctcgcc gaagtgaatc
      121 acaaaatcca ccgtattgaa tttaatgaaa tcaccaaaga tgcggttatt catgctgtgg
      181 agcatccacg ggacatcgac atggagcgtg taaactcgca acaagcgcgc cgcattttag
      241 accgtcttgt ggggtatgaa atttctcccg tactccaaaa aaaattcggt tcgcgccgtt
      301 tctcggctgg ccgagtgcaa tcggcggcgc ttaaaatttt atgcgaccgc gaaaaggaaa
      361 tcgaagcgtt tgtcccgcaa gaatactggg aagccgacgg tttttttgct acccccgatg
      421 cgaaagccaa agataaagaa acggcgttcc gcctggcgca aatcgacggc aagaaaacag
      481 aaatctacaa ccgcaccgaa gccgaaaccg cagaaaaaaa aatccgcagc gcaaagtata
      541 cgctcctcac acgcaagaca tccgaacgcc gcatcaaacc cctcgcgcct tacatcacca
      601 gccgcttgca acaagatgcc tcgactcggt taggattccg cgcacaaaaa acaatgtcga
      661 tagcccaaaa tttatacgaa ggcgtcgact taggcaaaga aggcacgcaa ggcctcatca
      721 cgtacatgcg taccgattca acgcgtatct cgcaaacggg aatcgagcaa gcgcgcgctt
      781 ttattagcgc aaactttcac gcagagcatt tgcctgagag cgcgaaccaa tacagcaaga
      841 aaaacgaaaa cacacaggat gcgcacgaag ccatccgccc caccgacgtg aatcgcactc
      901 atgatagcct tgagaaatat ctcgaccgcg accagtttcg tctctacgcg ctcatctggc
      961 ggcgctttgt tgcttcgcaa atgacaccgg gtgtggacga attggtgacg ctcgaagcgg
     1021 aaggtaaaac cggagacgcc gccgagacac cacagcaatt tcttttccgc tactccagct
     1081 cgcgtcaact tttcgacggc tttcgcgcag tgtacccttt gggcgacgag aaagcaggtt
     1141 acgtacctca ctttaacgag ggcgatgcgc tcaccctcat cgattttcaa aagcaacaaa
     1201 aattcacaca accaccaccc cgctacaccg aagcgtcgct catcaagtcg atggaggaat
     1261 cggggattgg ccgtcctgca acgtacgtgc ccacgatggc cactctcgac aaacgcgcgt
     1321 atatcgaacg caagggcaaa cagcttatcc ctaccaaatt agggcgcgtg gtaaacgaca
     1381 ttatggcggc gcacttcccc gacattgtca atttgcagtt cac
//
LOCUS       DAOMPD010000623         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33263_length_1423_cov_1.788012, whole genome shotgun sequence.
ACCESSION   DAOMPD010000623 DAOMPD010000000
VERSION     DAOMPD010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33263_length_1423_cov_1.788012"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..318
                     /locus_tag="PLY93_14835"
     CDS             130..318
                     /locus_tag="PLY93_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70798.1"
                     /translation="MDTGDKTHLKAKRRAAGNGTVVAVLAGEGSALAAGLALAAQRPW
                     IAKSVANSRIVIFFMLPL"
     gene            complement(334..684)
                     /locus_tag="PLY93_14840"
     CDS             complement(334..684)
                     /locus_tag="PLY93_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70799.1"
                     /translation="MMRHFYSYIVSAVIVVTGLTLGCSCNSKAEENIPKADAQPALPL
                     ETITGTVQVYDHNGNKQVVLELKRDKRSKITYEITGKNTDAVSHQKGKKLTVTGFVRN
                     LSPFHKLIDVQEIQ"
     gene            complement(687..>1423)
                     /locus_tag="PLY93_14845"
     CDS             complement(687..>1423)
                     /locus_tag="PLY93_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70800.1"
                     /translation="GTGVKNSFGMTIYFPSSAADYDTSYNSTILAQNTRWDEFIKGEI
                     NTYQENTTEPADATCGAEPNNTSTNAKDIAAKVGTSPTPCTGYIYTAGDVDFYKFGLS
                     GLPTTSGADVTKTIKVLLSNIPAGANFDVLVYAPNFSPSSPIAVGAAASSNGDEEFSI
                     ELGTGKVTYTKPNLSSYNGGVCDPSDSNNTLSNYGYCYGQGTVAASGTFKFYVVVVGK
                     NNSYSQLGKYTLTVTTGSGLTVTAAP"
BASE COUNT          309 a          318 c          404 g          392 t
ORIGIN      
        1 ggtaaccgcc gaggtagttg tcgctgcggc ggggggagaa gaaagtgaac cggcattggc
       61 ggcgttcatg gctactgtca tggaatcgac gtctttttgg atgagtgcgt ttgatccaat
      121 ggggcctgct tggataccgg tgacaaaacc cacttgaaag cgaaacggcg agcggcgggg
      181 aatgggactg tcgttgccgt gctggcaggg gaaggctctg ctttagcggc aggcttggct
      241 ttagccgccc aaagaccgtg gattgccaag agtgttgcta atagtcgcat cgttattttc
      301 ttcatgttac ctctttaatt ttattggagt gctttattgt atttcctgaa catcgatgag
      361 tttatggaat gggcttaaat tccgcacgaa accggtgaca gtgagcttct tgcctttttg
      421 gtgagaaacc gcgtcggtgt tcttgccggt gatttcgtac gtaattttag agcgcttgtc
      481 gcgtttgagt tcaagcacca cttgtttatt gccgttgtgg tcgtacactt ggactgtgcc
      541 cgtgatggtc tcaagtggca atgccggttg cgcatcggct ttggggatat tttcctcggc
      601 tttgctattg caagagcaac cgagggtgag ccctgttacc acaatgactg ccgaaactat
      661 ataagagtaa aaatggcgca tcatgattac ggagccgcag taacagtcag gccgctaccg
      721 gttgtgaccg tcaacgtata cttacccaac tggctgtacg aattgttttt acccacgacc
      781 acgacgtaga acttaaaagt accgctggct gcgacagtgc cttggccata gcaataaccg
      841 taattgctga gggtgttgtt actatcactg ggatcgcata ccccgccatt atacgaactt
      901 aaattgggtt tggtgtaggt gactttccca gtgccgagtt caatcgaaaa ctcttcgtcg
      961 ccgttactcg atgcggcggc accgaccgcg atgggtgacg acggactaaa attgggtgcg
     1021 tataccagca catcgaagtt ggcgccggcg gggatgttgg agaggagtac tttgatcgtt
     1081 ttggttacgt ctgcacccga agtcgtgggc agccccgata agccaaattt gtaaaaatcg
     1141 acatcgcctg cggtgtagat ataaccggtg caaggagtag gggatgtgcc cactttggca
     1201 gcaatgtctt tggcattcgt tgaggtgttg ttcggctctg cgccgcacgt tgcgtcggcg
     1261 ggctcggtgg tgttttcttg gtacgtgtta atctcacctt taataaattc atcccaccga
     1321 gtgttttgtg ctaaaattgt gctgttatag ctggtgtcgt agtcagccgc cgacgaagga
     1381 aaataaatcg tcataccgaa agaattttta acgcccgtgc ctt
//
LOCUS       DAOMPD010000624         1415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33479_length_1415_cov_2.256618, whole genome shotgun sequence.
ACCESSION   DAOMPD010000624 DAOMPD010000000
VERSION     DAOMPD010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33479_length_1415_cov_2.256618"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLY93_14850"
     CDS             complement(<1..264)
                     /locus_tag="PLY93_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70801.1"
                     /translation="MEASYAQSIIDELISGNFSRAAQLAAHELKNMPAYTGLTTTPER
                     SIAEEAYKITRYLLHRSERLANMPVGMDKGRAYQEILVDLSQVP"
     gene            659..>1415
                     /locus_tag="PLY93_14855"
     CDS             659..>1415
                     /locus_tag="PLY93_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HRP70802.1"
                     /translation="MNKLAFLTIAIFTLFVDTLAATVNPDGSFSTSIPIETPAGRNGI
                     QPKLALTYNSNAGNASASLSADSIVGIGWSVQGLPVITRINFSRGINYDGQDIYAGPE
                     GRLIDVSTSAGAATGSIYHAENQNWTKYEPLGSDGQTLTQSNRCGDSACSWRATDRNG
                     VVYFYGAAGSSRVTAVDAQGNAIHGGAVRVWALSRVTDLHGNYYEVEYYQNSGQFYPK
                     KILYTGNVAAGLPPRYSVRFSYDETGRPDKEISY"
BASE COUNT          336 a          347 c          379 g          353 t
ORIGIN      
        1 gggtacttgg ctgaggtcga ccaaaatttc ttggtacgca cggcctttat ccatacccac
       61 cggcatattc gcgaggcgtt cactgcggtg caatagatac cgtgtgattt tgtacgcttc
      121 ttcggcaata ctccgttcgg gggtcgtggt aagtcccgtg tacgccggca tgtttttgag
      181 ctcatgcgcg gctaactgcg cggcgcgcga aaaattaccc gagatgagct cgtcgataat
      241 cgactgcgcg taagaggctt ccaccgtggg gcagggtgta ttgcaaagaa gcggcgtaaa
      301 tcacttagag gcgggtgcat catggagcct gtgcgccaac tcgttgatgt aatttctttg
      361 tgaattttcg tacgctattt tacacaaatt cccccgcgaa ttatgccaat tcgctctgcg
      421 agctattgga atttttgcaa aatttcgcat ttttgagcga accgcgtaca aaaatttgca
      481 aaaaaattgc ctctccataa acgctgcgcg agttggcgca caggctcata atatgtatta
      541 cacgaaatgt gcagcgcaca acaaataggt tgacgcacca tctcaaacct tgtcccaatc
      601 ctttcattct cacactctaa tggggtgtgg gcaaaataac gcgtgacgga gcttgccgat
      661 gaacaagctt gcttttttaa ccatcgctat ttttactctc tttgtcgata cgctggcggc
      721 gactgtgaat cctgatggga gtttttcaac cagtataccg attgaaactc cggcaggccg
      781 taatggcatt caacccaaac ttgccttaac atataattca aatgccggta acgcttcggc
      841 ttcgctcagc gcagattcta tcgtcggcat agggtggagc gtgcaaggct tacccgtcat
      901 tacgcggatt aatttcagcc gtgggattaa ctatgacggt caggacatct acgcaggccc
      961 cgaaggtcgg ctcatcgacg taagcacgtc ggcaggggca gccacaggct cgatttatca
     1021 cgcggaaaat caaaactgga caaaatacga accgctgggc agtgacggcc aaacgctcac
     1081 gcagagtaac cgttgtggag attctgcttg ctcgtggcgt gccaccgacc gtaacggtgt
     1141 cgtgtatttt tacggcgcgg cgggtagttc gcgcgttact gcggtcgatg cgcaagggaa
     1201 tgccatccat ggcggagcgg tgcgtgtgtg ggcgctctcg cgcgtgaccg atttgcatgg
     1261 aaactattac gaagtcgaat attaccagaa ttcggggcag ttttatccga aaaagatttt
     1321 gtacacgggc aatgtagcgg cggggttgcc gccgcggtac agcgttcgtt ttagctacga
     1381 tgaaacgggt aggcccgaca aagaaatcag ctatg
//
LOCUS       DAOMPD010000625         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33676_length_1408_cov_2.374723, whole genome shotgun sequence.
ACCESSION   DAOMPD010000625 DAOMPD010000000
VERSION     DAOMPD010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33676_length_1408_cov_2.374723"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="PLY93_14860"
     CDS             <1..1361
                     /locus_tag="PLY93_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRP70803.1"
                     /translation="TEDTLETSSAGGPKPSGSKSTNSDGDNKSNAAHDIGAGKKAFAE
                     KDFAGAEAHFKRALSVEPENADVRFFLARSLEAQGKNEATEAEYRRVLEKNPNHSKAN
                     YYLGRRLYQAKKFSESERHFARSAAADEKFAPAQYSLGLAREKQGKNDAALQAYKKAS
                     AINPSLTQAHFNSAILLKRQHKYNEALEELQKAGNGQDVHYQKGEILLKTKRYNEAKT
                     EFTQVLAVKPNHYEAAFNLALTYHKLKDPSGADRALANIMHDDSPADLHYTYGKLLEE
                     SGELKSAEKQYRLSVNKDPQYFNGWLNLGKVLVRTQNFSQAEEAYRKALGIDPSSYEA
                     NVNLANTLYRQKKYQNAIEYFEAAKQKEKSREIILPLAASYEETTQNEKAAKVYTDFL
                     AGHPQDSTALEHLAYLYYRKIKNNDKALETFNQLLKYHPNSKKANEYRAMVKLIEKKQ
                     AE"
BASE COUNT          467 a          324 c          325 g          292 t
ORIGIN      
        1 gtactgaaga tacgttagag acgagtagtg cgggtggacc aaagccttcg ggaagcaaaa
       61 gcacaaattc cgatggcgac aacaaatcaa acgctgcgca cgacatcggc gcaggcaaaa
      121 aagcgttcgc cgaaaaagat tttgctggcg ccgaggcgca ttttaagcgg gcgttgtcgg
      181 tcgagcctga aaatgccgat gttcgttttt ttctcgcgcg cagtctcgaa gcgcaaggca
      241 agaacgaagc taccgaggcg gaataccgcc gcgtgttgga aaaaaacccc aatcactcca
      301 aagcaaatta ttatttagga cgtcgccttt atcaagcgaa gaaattttca gaatcggaac
      361 ggcactttgc ccgttcggcg gctgccgacg aaaaatttgc tccggcgcag tattcgctcg
      421 gccttgcgcg tgagaagcag ggcaaaaacg acgctgctct ccaagcgtat aaaaaagcga
      481 gcgcgatcaa tccctctttg acgcaagcgc atttcaacag cgcaatttta ttgaagagac
      541 aacataagta taacgaagcg ttggaggaac tacaaaaggc cggtaatggt caagacgtgc
      601 attaccaaaa aggggagatt ctccttaaaa caaaacgtta caacgaagcg aaaacagaat
      661 tcacccaagt gcttgccgtg aaacccaacc actatgaagc ggcgttcaac ttagcgctaa
      721 cgtaccacaa actgaaagac ccgtcgggag cagatcgcgc tttagcaaat atcatgcacg
      781 acgattcccc tgccgattta cattacacgt atggtaaact tttggaggag tcgggagaac
      841 ttaaaagcgc cgaaaagcaa taccgattga gcgtcaataa agacccgcaa tactttaacg
      901 gttggctcaa cttgggtaaa gtattggtac gcactcaaaa tttttctcaa gccgaggagg
      961 cataccgtaa agcattgggc atcgatccct cttcttacga agcgaatgtg aatttagcca
     1021 acacacttta tcggcaaaaa aaataccaaa acgcaatcga gtattttgag gcggcaaaac
     1081 aaaaagaaaa gtcgcgcgag attattttgc cgttagcagc gagctacgag gaaacaacac
     1141 aaaatgaaaa agcggcgaag gtctatacag attttttagc aggccatcca caagactcga
     1201 cagcattaga gcatttggcc tatctctatt atcgaaaaat aaaaaataac gacaaagcgc
     1261 ttgaaacatt taatcagttg ttgaaatatc accctaactc aaagaaggca aacgaatacc
     1321 gagctatggt taagctcatc gaaaaaaaac aggctgagtg atatgtccgc atcgaatcaa
     1381 ctacggcaaa tccttctggc tttagaga
//
LOCUS       DAOMPD010000626         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_33699_length_1407_cov_3.380917, whole genome shotgun sequence.
ACCESSION   DAOMPD010000626 DAOMPD010000000
VERSION     DAOMPD010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33699_length_1407_cov_3.380917"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..509
                     /locus_tag="PLY93_14865"
     CDS             39..509
                     /locus_tag="PLY93_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70804.1"
                     /translation="MRRSFHPSFCRNVVSLLLVFVFTNALRAQEFHPTISLTATIPFA
                     KPYLGLTESSSLSLANAETGESTLRRGEIIFFISYPFTFLASFATYSLFGYSLSTLDN
                     HTAFTPDASFYILSALTAAFLSFGIALNDYNAIKAETQYRNGSMTGTMFFSHRF"
BASE COUNT          380 a          312 c          321 g          394 t
ORIGIN      
        1 gaccccgtgg gcacaagccc actatttgat acatgaacat gcgccgctct tttcacccaa
       61 gtttttgccg caacgttgtt tctcttttgt tggtgtttgt ttttacaaac gcactgcgcg
      121 cacaagaatt tcatcctaca atatctttga ctgcgactat tccttttgca aaaccgtatt
      181 taggattgac tgaatccagc tcgcttagct tagccaatgc cgaaacgggg gagtcgacac
      241 tgagacgtgg cgaaattatt ttttttattt cctacccgtt tacttttttg gcgagttttg
      301 caacgtatag cctctttggt tattcgcttt ccacactcga taaccacact gcttttacac
      361 ccgacgcttc tttttatatt ctgtcggcac tcaccgctgc gtttttaagt tttggtatcg
      421 ccctcaacga ttacaacgcc atcaaagcgg aaacgcaata ccgtaatggt agcatgacgg
      481 gcacaatgtt cttttcgcac cggttttgag tgagacaggc ggggattagc tttatcgaaa
      541 agcgggataa ctatcaggct gttaaaaaac agtccatata gcagggtgtt gagccgagca
      601 cagccgcaga caggccattt atggccgttt tgcgaggggt tgctaagggc aaccccggcg
      661 tctgcggcgc gcacggcatt tttcaacacc ctgctagtcc tgcttgtctc gagtatggat
      721 tatgtcacat tagggataac tatttttaag cattatgtcg cattcaggga taactatctc
      781 aaccaaaaaa tttcttaaaa aaaattagaa tttttcccaa aaatttgcac ttttttttcc
      841 aaaagtgaga aaatttgacc gttttttggt atttaatata tatagagagg caaatatgtt
      901 aaagacatcc atcagtttac caacaaaatc tatccctact tggtgtacaa accgctgggc
      961 aatcatgcga atgtatgcac ggttcttgag aattgtcgag cggaaaagag agtttcggcg
     1021 gaaagtaaca cgacaataca acagaggtaa tgggcattgc gggattgtga aggtacgatt
     1081 ttcggcgaaa gagtatgatt cgctgcatac tgtggctgcg ggaatacggg tgagtgtatc
     1141 gtatctcatc tattacctca tccaattttg ggaaaaagcg tccgaaaaaa gagtcaagtt
     1201 cgtagttggg ggcaactata cgcctaaggt gctggagtgg tcgaaaaaga gggtaaattt
     1261 cgaggaaaaa ctatccttca ggcgagcagg acatcgcttc tttgcacgac gaagtccgta
     1321 gcacctcaca ccacctattc gggttgtgag cggcgggcga gcaaaaagaa gaatgcggca
     1381 ctcaacgcgc tcaccacacc caatgct
//
LOCUS       DAOMPD010000627         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34043_length_1396_cov_2.639075, whole genome shotgun sequence.
ACCESSION   DAOMPD010000627 DAOMPD010000000
VERSION     DAOMPD010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34043_length_1396_cov_2.639075"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..942)
                     /locus_tag="PLY93_14870"
     CDS             complement(103..942)
                     /locus_tag="PLY93_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP70805.1"
                     /translation="MPYFNDNDQSMYYRASGKGKDALLFIHGWYQNGAQAWSQQMGRF
                     EKKYQVFIPDLLGHGMSALENPEAFSIATNRRILLAFIDYIKKKYKVRRVVLLGHSYG
                     AFAALDLANEHTREIHGVVAMAAVDDYAPYVGELKKILRIPFFFGFLYYRIQALVGLF
                     PYGDRPLLYNKNTPSLYPSRLAYAKIKNKTLSLKNSRAYMSAFVSAKIKWSEKKIPLP
                     LFLIYGNRDALTPVKHAARIRPHFNNASLLLVEDAGHNVQISGAEKIEKPLLKFFEKC
                     FRT"
     gene            complement(944..>1396)
                     /locus_tag="PLY93_14875"
     CDS             complement(944..>1396)
                     /locus_tag="PLY93_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70806.1"
                     /translation="IAVAASGSFPGGGKNSEENSIEGFGALGAFFPFGVGGVFEVYGG
                     AGGGRYHINGIYSTSQRYYGQFNLGYTSDFFDIAFITRMAYTHLNSYQDWVAVSGFSF
                     EPGLTLRLGIKNVKLESQLMVPFSRNAYNDLKIVPLMFSLGAQTAFNF"
BASE COUNT          385 a          326 c          311 g          374 t
ORIGIN      
        1 atagtggcaa tcgatagtga ctattttttg catgcgccat ttagatagtt ggaacgtgtg
       61 cgtcaactta ttgggttgtg tgtttaatag agactcaaaa agctatgtgc gaaaacattt
      121 ttcaaaaaat ttcaaaagag gcttttcgat tttctcagcc cccgaaattt gcacgttgtg
      181 tccggcgtcc tcgacaagca atagcgacgc gttattaaag tgcggacgaa tgcgcgcggc
      241 gtgtttcaca ggggtgagcg cgtcacgatt gccataaatc aaaaaaagag gcagtgggat
      301 ttttttctca ctccatttta tttttgcaga tacaaaagca ctcatatacg cacgggaatt
      361 tttgagcgaa agggttttat tttttatttt cgcatacgct aagcgactcg ggtaaagcga
      421 tggtgtgttt ttgttataca agagcggtct atcgccgtac gggaataatc ccactaacgc
      481 ttggatgcgg tagtataaga aaccgaaaaa aaacggtata cgtagaattt ttttaagctc
      541 acccacgtac ggcgcatagt cgtcgacggc agccatcgcc accacaccgt gtatttctcg
      601 cgtgtgttcg tttgcgaggt cgagcgcggc aaacgcaccg tacgagtgac cgagcaaaac
      661 cacacggcgc actttatatt tttttttgat gtagtcgata aatgcgagga gtattctacg
      721 gtttgttgca atcgaaaatg cttctggatt ttccagtgcg ctcatcccat gccctaaaag
      781 atcgggtata aacacttggt attttttttc aaagcgcccc atttgttgcg accacgcctg
      841 cgcaccgttt tggtaccaac cgtggataaa aagaagcgcg tctttgccct ttcccgacgc
      901 acgatagtac atcgattggt cgttgtcgtt aaagtaaggc acgttaaaaa ttaaacgcgg
      961 tttgcgctcc cagtgaaaac atgagcggta caatttttaa atcgttgtac gcattgcgcg
     1021 agaaaggcac catcaactgc gactccagct tgacgttttt tatccccagg cgtaacgtca
     1081 atccgggctc aaaagagaat ccggacaccg ctacccaatc ttggtaggaa ttcaggtgtg
     1141 tatacgccat gcgtgttatg aacgcaatat caaaaaaatc gctcgtgtaa cctaaattaa
     1201 attgaccata ataccgttgc gacgtactgt aaatgccgtt tatatgataa cgccctccgc
     1261 cagcaccgcc gtacacttca aaaacaccgc caacgccaaa gggaaaaaaa gcgcccaacg
     1321 caccaaaacc ttcgatagaa ttttcttcgc tgttttttcc acctcccgga aaagatcccg
     1381 acgctgccac agcaat
//
LOCUS       DAOMPD010000628         1394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34115_length_1394_cov_2.627334, whole genome shotgun sequence.
ACCESSION   DAOMPD010000628 DAOMPD010000000
VERSION     DAOMPD010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34115_length_1394_cov_2.627334"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLY93_14880"
     CDS             complement(<1..887)
                     /locus_tag="PLY93_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70807.1"
                     /translation="MRGVRNIFLLALPWLFACGQKLDLFLNEDKEIVLTIESETTMSP
                     TISIVEGKFHFNVTFHLDRKGNYYFYHGGSCNVDNNFLITSGTLIKDTAVSVKVGITF
                     SNITAHGKTVTLCVEDTAAYKKKSHSFDFSGVLTSIAQFINEGIGSYSLSNSSSTTTE
                     FVLFQNDWTGVNPHRSTGSVVQNGVQLPRYHTAFIDPNDGYRLKYAVVDTANHRVLLF
                     NKPPTAGFDSANVVVGQTSFIAGNTNAGGVVSCSGFDTPTHVSVSASGVLFVTDASNN
                     RVLGFGQAPTTNGASANFV"
     gene            complement(897..>1394)
                     /locus_tag="PLY93_14885"
     CDS             complement(897..>1394)
                     /locus_tag="PLY93_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRP70808.1"
                     /translation="DLAQIIAAQKSRWFIATVPGAIAFLIFLVAMFAETNRLPFDLAE
                     AESELVTGFHTEYGAFKFALFFIAEYMNMITLSLFAALLFFGGYNIPAFFPEAVKVAW
                     YAPIIGSLLFFLKAFLFVFLFVWVRWSLPRFRYDQLMSLGWRRLVPLAMANLLIAGFW
                     VYFFP"
BASE COUNT          419 a          328 c          325 g          322 t
ORIGIN      
        1 aaaacaaaat tagcgcttgc gccattggtt gtcggcgctt gaccaaagcc caacacgcgg
       61 ttgttacttg catctgttac aaaaagaact cctgaagcag aaacagaaac gtgcgtagga
      121 gtgtcgaacc cactacatga gactaccccg cccgcgtttg tattacccgc gataaagctg
      181 gtttgcccca caaccacgtt ggcgctatca aatcccgccg tcggcggttt gttgaaaagg
      241 agcacacggt ggtttgcggt atccacgact gcgtatttca aacgatagcc atcgttgggg
      301 tcgataaacg cagtgtggta gcgtggcaac tgcaccccgt tttgtacgac actgccagtg
      361 ctccggtggg ggttaacgcc tgtccagtcg ttttgaaaga gcacaaattc cgtcgttgtt
      421 gaagaactat tcgacaagga ataactaccg atgccttcat tgataaattg agcgatggaa
      481 gtcagtacgc cactaaagtc aaaactgtgg gattttttct tgtacgcggc agtgtcctcg
      541 acgcagagcg taacggtttt accgtgcgcg gtgatgttgg aaaaagttat tcccacttta
      601 acagacacag cagtatcttt aattaaggtt ccagaggtaa tgagaaaatt gttatctaca
      661 ttgcacgatc cgccgtggta aaaataataa tttccttttc gatcgagatg gaacgttaca
      721 ttaaaatgaa atttcccctc aacgatagaa attgtggggc tcatcgtcgt ctccgattca
      781 atcgtgagta caatttcttt atcttcattc agaaaaagat cgagcttttg tccgcacgca
      841 aagagccatg gtagcgccag taagaatatg ttacggacgc cacgcatatt gactcattac
      901 ggaaaaaaat acacccagaa tcctgcaatc agtaaattcg ccatcgctaa aggcaccaaa
      961 cgccgccatc ccaaactcat gagttgatcg taacgaaaac gcggtagcga ccagcgtacc
     1021 cacacaaaaa gaaacacaaa gaggaacgct ttaagaaaaa agagaagcga acctataatg
     1081 ggtgcatacc acgccacttt gaccgcttcg ggaaaaaacg cagggatatt gtatccgcca
     1141 aaaaacaaga gcgcagcgaa caaggaaagc gtaatcatgt tcatatattc ggcgataaaa
     1201 aaaagagcaa acttgaatgc accgtattcg gtgtggaacc ccgtcacgag ttcgctttcc
     1261 gcctcggcca agtcgaaggg gagtcggtta gtttcagcga acattgcgac gagaaaaatc
     1321 aaaaatgcaa tcgctcccgg cactgtggcg ataaaccagc gcgatttttg tgccgcgata
     1381 atttgagcaa ggtc
//
LOCUS       DAOMPD010000629         1392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34179_length_1392_cov_2.513837, whole genome shotgun sequence.
ACCESSION   DAOMPD010000629 DAOMPD010000000
VERSION     DAOMPD010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34179_length_1392_cov_2.513837"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLY93_14890"
     CDS             <1..517
                     /locus_tag="PLY93_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRP70809.1"
                     /translation="KQLVVACASAVASLKGPLHGGANEAAMQMFLEVQKTGGPSTAES
                     YIMGKLQRKDKIMGFGHRVYMKKYDPRAFLMKDYIPKLVDQRPDGKDLYSIYQTIEKT
                     MAREKGLYPNADYPIALLYYLINVPIDLYTPIFLCSRLAGLVSHHIEQHENNRLFRPR
                     VIYEGARDLHP"
     gene            complement(997..>1392)
                     /locus_tag="PLY93_14895"
     CDS             complement(997..>1392)
                     /locus_tag="PLY93_14895"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRP70810.1"
                     /translation="IERNGRILLVETLEEAIEISNRYAPEHLEIQTEQNETLLRQIKA
                     AGSVFVGDYAPVAAGDYYSGTNHILPTAGASRFASGVSVFSFYRRITYQKLTHAGLFA
                     GKDAIAQMSKAEGLFAEHGYSVLTRFENS"
BASE COUNT          390 a          330 c          306 g          366 t
ORIGIN      
        1 gaaacaattg gtggtcgcgt gcgcgagcgc ggtggcatct ctcaaaggac cactccacgg
       61 cggcgcaaac gaagcggcga tgcagatgtt cctcgaagtg caaaaaactg gcggtccatc
      121 aaccgctgaa agctatatca tgggaaaact ccaacgtaaa gacaaaatca tgggatttgg
      181 ccaccgtgtg tatatgaaaa aatacgatcc gcgggcattc ctcatgaaag actacattcc
      241 taaacttgtc gaccaacgcc ccgacggtaa agacctttac tcgatttacc aaacgattga
      301 gaaaactatg gcgcgtgaga aggggcttta ccctaacgca gactatccta tcgcgcttct
      361 ttactacctc atcaacgtgc cgatcgacct ctatacaccg attttcttgt gctcgcgttt
      421 agcgggtctc gtgtcgcacc acatcgagca gcatgagaac aaccgccttt ttcgcccgcg
      481 tgtgatttac gaaggtgcac gggacttaca tccgtagtcg gatgttccat ggatgcaaaa
      541 ttcgggcatt tcgagaactg agatgaccga attttgctaa atttcttgat ttttaatggt
      601 ctttgcgacg tgagaagcaa gtctctgttt gcaaaggttc tcgaagtgac cggagcgtct
      661 acataaaaag aaactgcaca tttttccctg attttgatca ttccagcgcg tgcagtatat
      721 aattaccccc taatgtgaca taatttaaaa aatcgacata gttaccccct aatgtgacat
      781 aatcttaaaa attggcatag ttacccctta atgcgacata attctgtgga taaaatttcg
      841 aaatctaaac taaagtataa acccaggaat tcgtatgaaa tggatgcccc caaaaaaggg
      901 acgtcctaat acgtattccg aaccaacgca aggagtgaac attctctttt tatcgttgta
      961 aaatagttat ccccagcctt tcgttataca aaaaatctat gaattttcaa aacgggttaa
     1021 gaccgaatag ccgtgttcgg cgaacaatcc ctcggctttt gacatttgag caatcgcatc
     1081 cttgcctgca aaaagccctg cgtgcgtgag cttttggtat gtgatgcgtc gataaaaaga
     1141 gaagacgctc actcccgagg caaagcgtga tgcgcccgca gtgggtaaaa tatgattcgt
     1201 accggagtaa tagtcacccg ccgccactgg cgcatagtca ccgacaaaaa cactccccgc
     1261 tgctttaatt tgtcgcaaga gtgtttcgtt ttgttccgtt tggatttcta aatgttcggg
     1321 ggcatagcgg ttcgatattt ctatcgcttc ttctaaagtc tcgacgagga gaatacggcc
     1381 gttgcgttct at
//
LOCUS       DAOMPD010000630         1391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34215_length_1391_cov_2.461826, whole genome shotgun sequence.
ACCESSION   DAOMPD010000630 DAOMPD010000000
VERSION     DAOMPD010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34215_length_1391_cov_2.461826"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PLY93_14900"
     CDS             complement(<1..>1391)
                     /locus_tag="PLY93_14900"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRP70811.1"
                     /translation="YCFRYDLISGKWIEETACRDQTNSGGGLIGTYNYPAGQNGFLEP
                     RNEEYGPTIYGAASSIINGRRFRKDQNPLNLPPTGCTGGGNDACYPYTLDSKTNLIWR
                     TCSQGQYYNSVSGGGTLCGPTSGADYTWGDAVNACAALNNAANGTGYAGRRDWRLPTI
                     EELENLVDFGARITKSTTMPEPNPGYPEVPALDTYQETVWPLNSEGAFPNTPINKGYW
                     TATGVTMLFSGQVLYNQAYTVEFKKGSTGPGPGSNPLLNNKRDPSDFANRNKVRCVAG
                     PLVAPTPPTLTPSTVAASAVLVGNAATFTAPDGGASFNVKSVKPVYNIENNKSTVIVE
                     YDKVPDSTQASTAANYCIAPANAVACGPSSPLVTTIAQVTGNTSAFELTLQTYMGSNT
                     AYKIFVSNVQTKSNQWQTGVNYGVNQFVTDSGNVYRAANAHLSTTIAADVGAGDLVLL
                     NNLVTGTTYSTVG"
BASE COUNT          312 a          343 c          367 g          369 t
ORIGIN      
        1 ccccaccgtt gaatacgtgg ttcccgtcac gagattgttt agtaagacta aatcgccagc
       61 acccacatct gccgcaatcg ttgttgaaag gtgtgcgttc gcagcgcgat agacattccc
      121 tgaatcggtg acaaattggt tgacgccata gttgacaccc gtttgccact ggttgctctt
      181 cgtctgcaca ttcgagacaa aaattttata cgcagtgttg ctgcccatgt acgtttgcag
      241 cgttaactca aacgccgaag tattgcccgt gacttgggca atggttgtga caagggggga
      301 tgagggaccg caggcgacgg cgttggcggg ggcgatgcaa taatttgcag cagtgctcgc
      361 ttgtgttgag tcgggcacct tgtcgtactc cacgataact gtactcttgt tattctcaat
      421 gttataaaca ggtttaacgc tctttacatt gaaagacgcc ccaccatcag gggcggtaaa
      481 agtggccgca ttccccacca acactgcgct tgccgccaca gtggatggag tcagcgttgg
      541 gggagtgggg gcaaccaagg gaccggcgac gcagcgtact ttattgcggt ttgcaaaatc
      601 gctggggtct cgtttattat tgaggagagg gtttgaacct gggcctgggc cagtggagcc
      661 cttcttgaat tcgacggtgt acgcttggtt gtaaagaact tgaccactga aaagcatcgt
      721 cacacccgta gccgtccaat agcctttgtt gatgggggtg ttcggaaatg ccccttctga
      781 attaagcggc cataccgttt cttggtaagt atcgagagcg ggaacttcgg ggtagccagg
      841 gtttggttct ggcattgtgg tacttttagt gatacgtgct ccaaagtcca ccaagttttc
      901 cagctcttcg attgtgggta accgccaatc ccgccgcccc gcataacccg ttccgttcgc
      961 tgcgttattg agtgcagcac aagcgttgac ggcatccccc catgtataat cggcccctga
     1021 cgttggtccg cagagtgtac caccgccaga gacactgttg taatactgtc cctggctaca
     1081 agtgcgccat atgagattcg tttttgaatc cagagtataa ggatagcaag cgtcgtttcc
     1141 tcccccagta catcctgtag gtggtaaatt tagaggattc tggtctttcc taaagcgtcg
     1201 accgttaata atactcgatg cagcgccgta tattgtgggg ccatattctt cgttgcgagg
     1261 ttcgagaaaa ccgttttgcc ccgcaggata attgtatgta ccaataagac ctccaccact
     1321 gttggtttga tcgcggcagg cagtttcttc aatccatttg cctgatatta gatcgtaacg
     1381 aaaacaataa t
//
LOCUS       DAOMPD010000631         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34307_length_1388_cov_2.473368, whole genome shotgun sequence.
ACCESSION   DAOMPD010000631 DAOMPD010000000
VERSION     DAOMPD010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34307_length_1388_cov_2.473368"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>953
                     /locus_tag="PLY93_14905"
     CDS             <1..>953
                     /locus_tag="PLY93_14905"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HRP70812.1"
                     /translation="TCLGPSHGFDPYENTSGFIIWLNHSGIMVDPPVNTTEWLEKSNV
                     NPKFIDSIILTHCHADHDAGTFQKILEEGKVTIYTTETVISSFLKKYSAFANIDESYL
                     RRLFAFKPVKVGQPFFIHGGRFDVFYTLHSIPTVGFQLKFQDQTFVYSSDHNNQPEIH
                     DKLKELGVVSESRYNALRNFPWNSDVIYHESGIAPLHTPVAYLDSLPDDVRKRIVVYH
                     ISAKDFPKETLLTLAKFGIENTLYFNATEPENVKASQILGTLKQLDFFGDMPISRSQE
                     FIDIVQVEKYNKGDVIIRQGTQGDRFYIIYFGSVSVRTDDL"
     assembly_gap    954..976
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <977..>1388
                     /locus_tag="PLY93_14910"
     CDS             <977..>1388
                     /locus_tag="PLY93_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRP70813.1"
                     /translation="KIYGAYDYFGEVALVTNQMRTADVIAETEVTLFSIEKYKFLNFI
                     RGSDFEKILHRLARVRDAETWNVISQSRHLDFLTSTQKTWLESMLVPIEIKDASDIYT
                     ENAVPQYFYIIRAGSIIVTKDDKPLTTLTRGDVVI"
BASE COUNT          363 a          319 c          311 g          372 t
ORIGIN      
        1 tacttgcttg gggccgagcc atgggtttga cccttatgaa aatacatcgg gttttatcat
       61 ttggcttaac cattcgggca tcatggtcga tccccccgtc aacacgaccg aatggttgga
      121 gaaatcgaat gttaatccca agtttatcga tagcattatt ttaacgcact gccatgccga
      181 ccacgacgcg ggtacgtttc aaaaaatttt ggaagagggt aaggttacca tttacacaac
      241 ggagacggta atcagtagct tcttgaaaaa atactccgcg tttgccaaca tcgatgaaag
      301 ttatttgcgc cggctttttg cgtttaagcc ggtgaaagtc ggccaaccgt tttttatcca
      361 tggaggtcgg tttgatgttt tctatacgct gcattcgata ccgacggtgg gatttcaact
      421 taaattccaa gaccaaacgt ttgtctattc ctccgaccac aataaccaac ctgagattca
      481 cgataaactc aaagaattgg gtgtggtgag cgaatcgcgt tacaacgccc tgcgcaattt
      541 cccgtggaac agcgatgtga tttaccacga gtctggtatt gcgccgttgc acacgccggt
      601 ggcgtatttg gattctctcc ctgacgacgt gcgcaagcgt attgttgtgt atcacatcag
      661 cgcaaaagat ttccccaaag agacactgtt gacgctcgct aagtttggta tcgagaacac
      721 gctttacttc aacgccaccg agccagaaaa cgtaaaagcg tctcaaatct tagggacgct
      781 taaacagttg gatttcttcg gcgatatgcc tatttctcgc tcgcaagagt ttatcgatat
      841 cgtacaagtt gaaaagtata ataaaggcga tgtgataatc cgccaaggta cacaaggcga
      901 tcggttctat attatttatt ttggctcggt gtcggtgcgc accgacgatt tgcnnnnnnn
      961 nnnnnnnnnn nnnnnnaaaa tttatggcgc gtacgattat tttggcgaag ttgcactcgt
     1021 cacaaaccaa atgcgcaccg ccgatgttat cgccgagacc gaggtgacgc ttttttccat
     1081 cgagaaatat aaatttttga atttcatccg tggatccgat tttgaaaaaa tactgcaccg
     1141 cttggcgcgt gtgcgtgacg cggaaacgtg gaacgttatt tcgcaaagcc gccatttaga
     1201 tttcttgact tcaacgcaaa aaacatggct cgaatcaatg ctcgttccga ttgaaattaa
     1261 agatgccagt gacatctata ccgagaatgc cgtgccacag tacttttata tcattcgagc
     1321 cggttcaatc atcgtcacca aggacgataa accgcttacc acactcacgc gcggcgacgt
     1381 ggtgattc
//
LOCUS       DAOMPD010000632         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34396_length_1385_cov_2.188722, whole genome shotgun sequence.
ACCESSION   DAOMPD010000632 DAOMPD010000000
VERSION     DAOMPD010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34396_length_1385_cov_2.188722"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /gene="recR"
                     /locus_tag="PLY93_14915"
     CDS             <1..510
                     /gene="recR"
                     /locus_tag="PLY93_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRP70814.1"
                     /translation="FVKKGPVRVSNFTHTLEKLASGVATCAECGAFCSAVEKNCTFCV
                     SSKRNFDTLCVVANASDIYAVENTGEHHGLYHVLGGVLSPLDGVGPEELNIPKLFERV
                     KSLHIKEVIVATNPSVEGNATGHYIADAVKPLGVRVTRIATGLALGQLLDYAETQAIS
                     QSLRQRVEL"
     gene            544..>1385
                     /locus_tag="PLY93_14920"
     CDS             544..>1385
                     /locus_tag="PLY93_14920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRP70815.1"
                     /translation="MTIFTFTIRAEGVVYEWPAEKDKKNQISSTFGESRLDHFHNGID
                     LPGKGAKILAPRDGRVLYRVNADFTPGEMPFGGGNTLILEHGTSASTHWTGYMHLTTF
                     ADSVLEDNPISTGDKIGVTGDTGHSGGPHLHFFIYAPHERTMLNPLLFMNDLYYDDTL
                     APEIKEWGVLLNDKFSTVNPKKPFRLSQDYPVYIFLQDHGRGKERWGVFDYRVVLDNK
                     EVLKTTFDKIFFKDGEWVLSSGHKFEDIFYRNFYSLTRQLRRSKEVFVEAKDIKGNTF
                     SQKYT"
BASE COUNT          363 a          337 c          328 g          357 t
ORIGIN      
        1 tttgtcaaaa aagggcctgt gcgcgtcagc aattttacgc acacgttgga aaaactcgcc
       61 agcggtgtcg ccacgtgcgc cgagtgtggt gcgttttgta gcgctgtgga aaaaaactgt
      121 actttttgtg tatcttccaa gcgcaatttc gatactctct gcgtcgttgc aaatgcctct
      181 gacatttacg ccgtcgaaaa taccggcgag caccacggtc tttaccatgt tttgggtgga
      241 gtactttcgc cgctggacgg tgtcggcccc gaagaactca atatccccaa actctttgaa
      301 cgggtgaaat cactccatat taaggaagtg attgttgcta ccaaccccag cgtcgaaggg
      361 aatgccaccg gtcactatat tgccgatgcg gtaaaaccat tgggggtgcg cgtcacgcgc
      421 attgccaccg ggcttgcgct gggacaactt ttggactatg cagagacgca agcgatttca
      481 caatcgctac gccaacgggt ggagctataa cggatgcgac aaagtttttt aaaatttact
      541 tttttgacta ttttcacttt taccatcaga gcagaaggtg tcgtctacga atggccggcg
      601 gaaaaagata aaaaaaacca aattagttcc accttcggcg agtcgcgcct cgaccacttc
      661 cacaacggta tcgatctccc gggtaaagga gccaaaattc ttgcaccgcg cgatggccgt
      721 gttctttacc gggtgaacgc cgatttcact cctggagaaa tgccgttcgg gggggggaat
      781 actcttatct tggaacacgg gacgtccgca tctacgcact ggacggggta tatgcacttg
      841 acgacctttg ccgatagtgt ccttgaggac aaccccattt ccaccggcga caaaatcggc
      901 gttaccggcg acaccggcca ctcgggtgga ccacacttac acttttttat ctatgccccc
      961 cacgaacgca cgatgctcaa tcccctactc tttatgaacg acctctatta tgacgacaca
     1021 cttgcgcccg agataaagga atggggagta ctcttaaacg ataaattttc gacggtcaat
     1081 cccaagaagc ctttccgctt gagtcaagat tatcccgtgt atatttttct gcaagaccac
     1141 ggccgaggta aggagcgttg gggtgttttt gattaccgtg tggtattaga caataaagaa
     1201 gtattgaaaa caacgttcga taaaattttt ttcaaggacg gcgagtgggt tttaagctca
     1261 ggtcacaaat ttgaagatat tttttatcgc aatttttatt cactcaccag gcaattacgc
     1321 cgcagcaaag aagtgtttgt cgaagcaaaa gatattaaag gaaatacttt ttctcaaaaa
     1381 tatac
//
LOCUS       DAOMPD010000633         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34420_length_1384_cov_2.787810, whole genome shotgun sequence.
ACCESSION   DAOMPD010000633 DAOMPD010000000
VERSION     DAOMPD010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34420_length_1384_cov_2.787810"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1384)
                     /locus_tag="PLY93_14925"
     CDS             complement(<1..>1384)
                     /locus_tag="PLY93_14925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP70816.1"
                     /translation="NFYTKFERFVADKAVDNNNISTDKMDADQVANYDLAWIAAEIFA
                     IDEMLEYPTKVGKEGDTIETGLARFFIADALTDILARLRLAAHKMGVLPVDFENEIHS
                     AEIQTFAMQYASEAEAKKITDLLAANKDFGAYGLNEDQAMMADTFRKFAEGQVKPIAE
                     KVHRQDLTIPDEIIDGLAEMGCFGLSIADTYGGFQSEENPDHTSMAVVTEELSRGSVG
                     VAGSLITRPEIVAKAISAGGTEEQKQKWLPLLASGEKKCAVAVTEPDYGSDVAGMKVA
                     AVKVDGGWKINGVKTWCTFAGMADVMLVLARTNPDMKLKHKGLSILLAEKPTTRDHEF
                     DYTQPTGGRCHGKAISTIGYRGMHSYEVVFEDYFVPDENLIGGEAGLGKGFYLQMAGF
                     AGGRLQTAARATGVMQAAIEKALQYALDRKIFGKPIIAYGINHFKVAKMAAITQAVRQ
                     MTYKSARLMDK"
BASE COUNT          315 a          362 c          332 g          375 t
ORIGIN      
        1 gcttgtccat gaggcgcgcc gatttatacg tcatttggcg cactgcttgc gtaatcgccg
       61 ccatttttgc gactttgaaa tgattgatgc cgtacgcaat gatgggctta ccaaaaattt
      121 ttcggtcgag cgcgtattgc agcgcttttt caatcgcggc ttgcatcaca ccagtggcgc
      181 gtgcagcggt ttgcaaacgc cccccggcaa aaccggccat ctgtaaataa aagcctttgc
      241 ctaagcccgc ttctccgcca atgagatttt catcggggac aaaataatct tcaaaaacga
      301 cttcatacga gtgcatgcca cgatagccga tagtcgaaat tgctttgccg tggcagcgac
      361 cacctgtggg ttgcgtataa tcgaattcgt gatcgcgagt cgtaggtttt tccgctaaga
      421 ggatagataa gcccttgtgc ttgagtttca tatcggggtt ggtgcgcgcc aaaacgagca
      481 tcacgtcggc catgccggcg aaggtacacc aggttttcac accgttgatt ttccagcctc
      541 cgtcgacttt cactgcggca actttcatac ccgccacgtc tgaaccatag tcgggttcgg
      601 tgacggcgac tgcacatttc ttttcacccg acgctaaaag cgggagccac ttttgctttt
      661 gctcttcggt accacccgct gaaattgcct tcgccacaat ttcgggacga gtaatgaggc
      721 ttcccgccac gccgacgctg ccgcgcgaca actcttcggt gacgacggcc atcgatgtgt
      781 ggtcgggatt ttcttccgac tgaaatcctc cgtaagtgtc ggcaatcgac agaccaaaac
      841 aacccatttc agcaaggccg tcgataattt catcgggaat ggttaaatct tggcggtgta
      901 ctttctcggc gatgggcttt acttggcctt cggcgaattt acggaatgta tcggccatca
      961 ttgcttggtc ttcattcaaa ccatacgcgc caaaatcttt attggcggcg agtaagtcgg
     1021 tgattttttt cgcttctgct tcagaggcgt attgcatcgc aaacgtctga atttccgccg
     1081 aatggatttc attttcaaaa tccaccggta agacacccat tttatgcgcg gcaaggcgca
     1141 accgtgcgag aatatcagtg agagcgtcgg cgataaaaaa acgggctaaa ccggtttcaa
     1201 tcgtatcgcc ttctttccct actttagtag ggtattcgag catttcgtcg atagcaaaaa
     1261 tctcggcggc aatccaagcg aggtcataat tagccacctg gtctgcgtcc attttgtcgg
     1321 tcgagatatt gttattatct accgctttat ctgccacaaa tctctcaaac ttggtgtaga
     1381 aatt
//
LOCUS       DAOMPD010000634         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34502_length_1382_cov_1.691786, whole genome shotgun sequence.
ACCESSION   DAOMPD010000634 DAOMPD010000000
VERSION     DAOMPD010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34502_length_1382_cov_1.691786"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>555)
                     /locus_tag="PLY93_14930"
     CDS             complement(<1..>555)
                     /locus_tag="PLY93_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70817.1"
                     /translation="AELELWNGRSEFPTNIESMIEHGVNANMDRKGFLAFMGASISMA
                     TLAACREPVEKIVPYIDRPMEYIPGEARYFATTRATARGVTPILVKTREGKPIKIEGL
                     AGHPVYKGGTTADTFAAIWDLYDPDRVHNPLKKANGVFAVSKWDIVIPEVASALKGKS
                     RVLSRTTFSPAENTARAKLGKVVV"
     assembly_gap    556..565
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(697..>867)
                     /locus_tag="PLY93_14935"
     CDS             complement(697..>867)
                     /locus_tag="PLY93_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRP70818.1"
                     /translation="TKTACMQCHGDVSSMKVVEQKESLNMGFCVNCHKDYRNDVEYKN
                     HGVSTTCNTCHY"
     assembly_gap    868..883
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<884..1285)
                     /locus_tag="PLY93_14940"
     CDS             complement(<884..1285)
                     /locus_tag="PLY93_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRP70819.1"
                     /translation="MDSKIFKAIKYGTPVLFLAAAAWIIWAPPARIGYAPEQPIEYRH
                     DIHAGVAGQKTFDGKEQYGIDCQYCHTGVTTSKKAGVPSMNTCMNCHILVGGSLPGVK
                     KLKKFYEDKKPIEWIRIHNMPDHVRFAHAPHI"
BASE COUNT          301 a          321 c          335 g          399 t
ORIGIN      
        1 aaaccacgac tttacccaat ttggcccgcg ccgtattttc agcgggtgaa aatgtggtgc
       61 gcgaaagtac acgagatttc cctttaagcg cagatgcaac ttcggggata acgatgtccc
      121 acttgctgac agcgaaaaca ccgtttgctt tttttaatgg gttgtgtacg cggtctggat
      181 cgtaaagatc ccaaatcgcc gcgaaggtat cggcggtggt tcccccttta taaacagggt
      241 gaccagccaa gccttcaatt ttgatgggtt tgccttcgcg agtttttacc aaaatgggag
      301 tgaccccacg cgcagtggca cgggttgtgg cgaaataacg cgcctcaccc ggaatatatt
      361 ccatagggcg atcgatgtaa gggacgattt tctcaacggg ttcgcggcag gcagcgagcg
      421 tcgccattga aattgaggca cccataaaag ccaagaaccc tttacggtcc atgttggcat
      481 tcacgccgtg ctcaatcatc gattcgatat tggttggaaa ttcgctacga ccgttccaaa
      541 gttccagctc ggcttnnnnn nnnnnggtat cggatcccgg catttgcgcc tcttcgggcg
      601 tttgcgcggc tgtctcttca aaagagcgga agaacttaac aggttgaacc ttttttgttt
      661 cggtggatgt ttctttcacg tttcttcctc tttatgttaa taatggcaag tattgcacgt
      721 tgtgctaacg ccgtggtttt tatattccac gtcgtttcga tagtctttat ggcagtttac
      781 acagaacccc atgttgagcg attctttttg ctcgacaact ttcatgctcg agacatcgcc
      841 gtggcattgc atacaggcag tcttggtnnn nnnnnnnnnn nnnaatgtgg ggagcgtggg
      901 caaagcgtac atgatcgggc atgttgtgga tacgtatcca ttcaatgggc tttttatctt
      961 cataaaattt cttgagtttt ttaacgcctg gcaagctgcc accgactaaa atatggcagt
     1021 tcatacaggt attcatgctt ggcacaccgg cttttttgct ggtggttacg cctgtgtgac
     1081 agtattggca atcgataccg tattgctctt taccgtcaaa tgtcttttga cccgcaacgc
     1141 cagcgtggat gtcgtggcgg tattcaatcg gttgctcggg agcataaccg atacgagcag
     1201 gcggagccca gatgatccag gccgccgccg ctaaaaataa tacgggagtt ccgtacttta
     1261 tcgctttaaa aatttttgaa tccattcgga actccttttc tctcttaggt cttaccgccg
     1321 agttttaata cgtattcggc gaccgcttta atttcttcag ctgtcatgct atctttgtag
     1381 gg
//
LOCUS       DAOMPD010000635         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34529_length_1381_cov_1.843891, whole genome shotgun sequence.
ACCESSION   DAOMPD010000635 DAOMPD010000000
VERSION     DAOMPD010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34529_length_1381_cov_1.843891"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLY93_14945"
     CDS             complement(<1..412)
                     /locus_tag="PLY93_14945"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRP70820.1"
                     /translation="MPAKLIVGTQWGDEGKAKVIDYLAKETEIVVRYQGGANAGHTVK
                     VGDDKYIFRLIPSGILYPRTICVLGGGMVIDPDAMFDEIHELEAKGIQVTGRLRIADN
                     AHLLMPYHKQIDVLSEEPSGSHKIGTTKRGIGACY"
     gene            complement(419..946)
                     /gene="lspA"
                     /locus_tag="PLY93_14950"
     CDS             complement(419..946)
                     /gene="lspA"
                     /locus_tag="PLY93_14950"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRP70821.1"
                     /translation="MSQWRTKFTLPFFVVLVVVYVTDLVSKLWILKHFQVQGQSEAII
                     NNFLYLTFTRNEGGVFGMLQGYAWMFQILTGFAILFLIYYYYRSPESSKIFTVAIAAI
                     LGGALGNFTDRFYGETAGVIDFIDFRFGSFHWFIFNVADMFICIGAGLLAISFYQFEK
                     AEKLAVQQTKVAEGE"
     gene            1199..>1381
                     /locus_tag="PLY93_14955"
     CDS             1199..>1381
                     /locus_tag="PLY93_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRP70822.1"
                     /translation="MSKKVHVVKEDCTSCNLCSDTMPHYFRMDNDDLSETHNNGENIN
                     DAAVPETDYAKVQETID"
BASE COUNT          398 a          339 c          302 g          342 t
ORIGIN      
        1 aataacacgc cccaatgcca cgcttggtgg tgccgatttt atgcgacccc gatggctctt
       61 cactcaaaac gtcgatttgt ttgtggtatg gcataaggag gtgtgcattg tctgcaatcc
      121 gtaagcgacc ggtgacttga atacccttgg cttccaactc atggatttcg tcgaacatcg
      181 cgtcggggtc gataaccatt ccaccaccaa gcacgcatat tgtacggggg tataaaatac
      241 cgctggggat aagtcggaaa atgtatttat cgtcccccac tttaacagtg tggcctgcat
      301 tggcgccgcc ttggtagcgc acgacgattt cggtttcttt ggctaaataa tcgatgactt
      361 tggctttgcc ttcatcaccc cattgtgtac cgacgatgag ttttgcaggc atacggattt
      421 actctccttc ggcgactttg gtctgctgta cggctaattt ctccgctttt tcaaattgat
      481 aaaacgaaat agcaagaagc cccgccccaa tacagatgaa catatcggcg acgttaaaaa
      541 taaaccagtg gaatgaacca aaacggaaat caataaagtc aatcacccct gcggtttcgc
      601 cgtaaaagcg gtcggtaaaa ttccccaacg ccccccccaa aattgctgca atcgccacag
      661 tgaaaatttt ggaggattct gggctacggt aataataata aataagaaag agaattgcaa
      721 aaccggttag gatttgaaac atccacgcat atccttgcag cataccaaat acgccgcctt
      781 cgttacgagt gaatgtgagg tagaggaaat tgttgataat cgcttcgctt tgaccctgca
      841 cttgaaagtg cttcaaaatc caaagctttg aaacgagatc ggttacgtac accacaacca
      901 aaaccacaaa aaagggaagg gtgaactttg tgcgccattg agacatttcg gcatctttgg
      961 cctttgaaac accgctacaa gctaaatgct agggcgcgcg ctatatcagg tggggtcaat
     1021 agttttttct aatacactct gatgggtgtt aatggcaaaa gcctcgcaat ttgaagacaa
     1081 attgctgcta aagcggtgaa gacacctgct tttgccatca acacccccac ccgatatagc
     1141 gcgcgcccta aatgttatat aaattgacgc cttgtttcaa tttacatcct cagcggcgat
     1201 gagtaagaaa gtccacgtcg tcaaagaaga ctgtaccagt tgcaatttgt gctcggatac
     1261 aatgccacac tacttccgca tggataacga cgacctttcc gaaacgcaca acaacggtga
     1321 aaacatcaac gacgctgctg tgcccgaaac tgattacgct aaagtgcaag aaaccatcga
     1381 c
//
LOCUS       DAOMPD010000636         1379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34572_length_1379_cov_3.229607, whole genome shotgun sequence.
ACCESSION   DAOMPD010000636 DAOMPD010000000
VERSION     DAOMPD010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34572_length_1379_cov_3.229607"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLY93_14960"
     CDS             complement(<1..386)
                     /locus_tag="PLY93_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP70823.1"
                     /translation="MARRKKITGTIRKVFKKKQKSLRERLQQFFKKNRISARNEQMQM
                     VLEILELNEKRAVDILTAEVNLSSLPLNASLKEIEALFKKTGHSRLPVYEDKEGMRHY
                     VGVFFVKDLVAIKATERKNFSLASHLR"
     gene            complement(386..895)
                     /gene="ybeY"
                     /locus_tag="PLY93_14965"
     CDS             complement(386..895)
                     /gene="ybeY"
                     /locus_tag="PLY93_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRP70824.1"
                     /translation="MIQLTFVDAAKKSQSKNQTLLKYKEWLLALERALPSFRFKKNKK
                     IRKNQFEEILVQFVDDKTIRKINTQYRKIQKTTDVLSFSYSDAANAHFPHEPRGEIFI
                     STDTAEKQAAFFKIPLHEELMVLTIHGLLHILGYDHETSEKDKEEMRKQETKLLKKIG
                     ITSEALTER"
     gene            complement(892..1260)
                     /locus_tag="PLY93_14970"
     CDS             complement(892..1260)
                     /locus_tag="PLY93_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP70825.1"
                     /translation="MSLKNLLELRTETEKVGSQDVNFLTFSGKITSDNIYDLNSKVKT
                     IFENGVYNCVLDISELEYINSTGIALLLTITRTVEQNHGMIFLTKPTSFVQELFEMTD
                     LAGRFTIVDSVNAAREHFKN"
BASE COUNT          331 a          257 c          305 g          486 t
ORIGIN      
        1 cgtaagtgcg acgcgagaga gaaattttta cgctcggtcg ctttgatagc gacaagatct
       61 ttcacaaaaa aaacgcccac gtaatggcgc atgccttctt tgtcttcgta aaccggcaag
      121 cgagaatgcc ctgtcttttt gaaaagcgct tcgatttctt tgagcgaggc atttagtggc
      181 aaactggata gatttacctc ggctgtcaat atatcgaccg ctcgcttctc gttgagttct
      241 aaaatttcaa gaaccatttg catttgttcg ttgcgtgcgg agattcgatt ctttttaaaa
      301 aattgttgta gtctttcgcg caacgatttt tgtttcttct taaagacctt gcggattgtg
      361 ccggtgattt ttttacgtct tgccattatc tctctgtcaa tgcctcgctt gtgataccta
      421 tttttttaag tagttttgtt tcttgttttc gcatttcttc tttgtctttc tctgaggttt
      481 cgtgatcgta acctaaaata tgtaaaagac cgtgtattgt taacaccatg agctcttcgt
      541 gtaatggaat cttgaaaaac gccgcttgtt tttctgcggt gtccgttgaa ataaaaattt
      601 cccctctcgg ttcgtgtggg aaatgtgcgt ttgcggcgtc gctataactg aacgagagaa
      661 catccgttgt cttttgaatt tttcgatatt gagtatttat ttttcttata gttttgtcat
      721 cgacgaattg cacaagtatt tcttcgaatt gattttttct tattttctta ttcttcttaa
      781 aacggaaaga ggggagtgcg cgttcaagcg caagcaacca ttctttatat ttgaggagcg
      841 tttgattttt actttgcgac ttttttgccg catcgacgaa agttagttgg atcaattctt
      901 aaagtgttcc cgcgcagcgt tgacggaatc gacgatggta aagcgtcctg caagatctgt
      961 catttcaaag agttcttgta cgaaagaagt gggcttggtt aggaagatca tgccgtggtt
     1021 ctgttcaact gtgcgtgtaa ttgttaaaag aagggcgatg ccagtgctat tgatgtactc
     1081 gagttctgaa atgtctagta cgcaattata gacgccgttt tcaaaaattg tttttacttt
     1141 gctatttaga tcgtagatgt tgtcagaggt aattttgccg ctaaaggtta aaaaatttac
     1201 atcttgcgac cctactttct cggtttcagt gcgcaattca agtagattct taagactcac
     1261 tgacttattt ttacacggcg catgtgaaag caagcatttt gtaagatttg gttgggaagg
     1321 agcctgtgag cctggagcct gtgcgccaac tatcaatttt cgtgcacaaa tagaggagg
//
LOCUS       DAOMPD010000637         1376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34673_length_1376_cov_2.049205, whole genome shotgun sequence.
ACCESSION   DAOMPD010000637 DAOMPD010000000
VERSION     DAOMPD010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34673_length_1376_cov_2.049205"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1376
                     /locus_tag="PLY93_14975"
     CDS             13..>1376
                     /locus_tag="PLY93_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRP70826.1"
                     /translation="MSAVAVTDHGNMFGSIEFYEAAKAEGIKPIIGYEAYVAPGSRFE
                     KRQQDDLVDGRAYHLILLAKNQTGYKNLIKLASKAFTEGLYYKPRIDYELLSEYSEGL
                     VGTTACLAGEVNRLLYRQKTDKAKELAGRLNEIFGKGNFFLEIQNHGIPEQLEVAKGA
                     LELSKQLDIPLVLTNDSHFLNREDQKAQEVMLRIQMNKKIDEPLEFGFNDEFYVKSPE
                     EMFRLFPELPQAFHQTNEIAEMCNFEFEFGAPLLPDFEIPSAHGGAEAAVAKDGGETA
                     KKFFAEKNAIDNAIASAKNSESHSTVAKTSMLVGPKLEKSTNPLRDYFFAEAQAGLER
                     RFNGNVSEEHRKRLAYELGVIEGMGFEGYFLIVADFINYAKRNNIPVGPGRGSAVGSL
                     VAYSLGITDLDPLKYDLLFERFLNPSRKEIPDIDVDFCRDRREEVIKYVVQKYGEDHV
                     SQIIT"
BASE COUNT          386 a          331 c          336 g          323 t
ORIGIN      
        1 aaagaattgg gcatgagcgc tgttgcggtc accgaccacg gcaacatgtt tggatcgatt
       61 gaattttatg aggctgccaa agccgaaggg attaaaccca tcatcggtta cgaggcgtat
      121 gtcgctccgg ggagtcgttt tgaaaaacgc caacaagacg acttggtcga tgggcgtgcg
      181 taccacctta ttcttttagc aaaaaatcaa acgggttaca agaatctcat caagcttgcc
      241 tcgaaagcgt ttaccgaggg gctttactat aaaccacgca tcgattacga gttactctcc
      301 gagtatagcg aaggtcttgt gggcaccaca gcgtgtttag caggggaagt caatcgtctc
      361 ctttaccggc aaaagacaga caaggcgaaa gaactcgccg gacgtctcaa tgaaattttt
      421 ggcaagggta atttctttct cgaaatacaa aatcatggca tccccgagca gctcgaggtg
      481 gccaagggcg cgcttgaact ttccaaacag ttggatatac cacttgtact gacaaacgat
      541 tcgcatttct tgaaccgcga agaccagaag gcgcaagaag tgatgctgcg catccagatg
      601 aataaaaaaa tcgacgaacc attggagttt ggtttcaacg acgagttcta tgtaaaatca
      661 cccgaggaga tgtttcgtct tttccccgaa ctaccgcaag cgtttcatca aacgaatgaa
      721 atcgccgaaa tgtgtaattt cgagttcgag tttggcgcac cattattgcc tgactttgaa
      781 attccttcgg cgcatggagg cgccgaagct gcagtagcca aggatggcgg tgagacagcc
      841 aaaaaattct tcgccgaaaa aaatgccatc gataacgcta ttgcatccgc gaaaaatagc
      901 gaaagccatt caaccgtggc gaagacttca atgctggtcg gacccaaact tgaaaaaagt
      961 acaaatccat tgcgcgatta tttttttgcc gaggcgcagg cagggctcga acgtcgcttc
     1021 aacggcaatg tttccgaaga acaccgcaag cgtttagcgt acgaactcgg tgtcattgaa
     1081 ggcatgggct tcgaaggcta ctttctcatc gtcgccgatt ttattaacta cgcaaaacga
     1141 aacaacatcc ccgtgggtcc aggacgcggt tctgcggtgg gctcgcttgt cgcatattca
     1201 cttggaatca ccgacctcga ccctttgaag tacgacttac tctttgaacg ctttttgaat
     1261 ccgtcgcgga aggaaatccc cgatattgat gtcgacttct gccgcgatcg acgcgaagaa
     1321 gtgattaagt acgtggtaca aaaatacggc gaggaccacg tctcgcaaat catcac
//
LOCUS       DAOMPD010000638         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34754_length_1373_cov_2.308042, whole genome shotgun sequence.
ACCESSION   DAOMPD010000638 DAOMPD010000000
VERSION     DAOMPD010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34754_length_1373_cov_2.308042"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLY93_14980"
     CDS             <1..623
                     /locus_tag="PLY93_14980"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HRP70827.1"
                     /translation="SLLQVACADTLLTAEGFAGKPSGGATIAGTVSISLGGGLYETKL
                     DATSDSAWVYFDFENPSGITVANPSTNNDWDIAFKRFMIKVNGGASGNANVQVVALNA
                     DNFASRTQAPSPFTPATNDVVDAGDGDDACRPTPAGVLFAFLNSTATPNACWFSYSIG
                     HLTVRDMTYILRTYANKYYKIQIKAYYSETGTAAKYRFQWAEVTSP"
     gene            722..>1373
                     /locus_tag="PLY93_14985"
     CDS             722..>1373
                     /locus_tag="PLY93_14985"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRP70828.1"
                     /translation="MILNIYYALYAEKTSDLEKNTHANDSHKTTTSGTHNTTVDSATQ
                     EKEYIEKLNRRHKVVLSAQRSESRLADSVVQVEVIDKEQIREKGARTVTEALNNETGF
                     FIQRGIFGDNVQLQGLDSQYLLIMKDGERLNGRINGQYDTSRLRVDNVEQIEIVRGSS
                     SAIYGSDAIAGVVNIITARPKKPLIDAKLQGGNLGAADLSGSAVIPLGDFLVRGSGV"
BASE COUNT          383 a          354 c          317 g          319 t
ORIGIN      
        1 taagcttact acaagttgcc tgcgccgaca cccttcttac tgccgaaggt tttgcgggca
       61 aaccatcggg tggcgccacg attgccggta ccgtctccat aagtctgggg ggaggtcttt
      121 atgaaaccaa acttgacgcc acctctgaca gcgcttgggt atattttgat tttgaaaatc
      181 ccagcggtat aactgtcgcc aatccttcga cgaacaacga ttgggatatc gcattcaagc
      241 gctttatgat taaagtcaac ggcggcgcat cggggaatgc aaacgttcaa gtggtcgcgt
      301 tgaatgccga caactttgcc tcacgcaccc aagccccttc tccttttacg cctgcgacca
      361 acgatgttgt cgacgccggc gatggcgacg acgcttgccg tccaacaccc gcgggagtac
      421 tttttgcttt cttaaattct accgctacgc ccaatgcatg ttggttttcg tattcgatcg
      481 gccacttaac tgtgcgtgac atgacgtata ttttgaggac gtatgcaaat aagtattaca
      541 aaatacaaat taaagcgtac tacagcgaaa ccggcaccgc tgccaaatac cgcttccaat
      601 gggcggaggt gacttctcca tagcaaacta cacaggtatt taatccatta ttctttaaaa
      661 ctaaactaaa atgccaatat tacgacacct aaaggagctg ctcgagatag ttatcctgat
      721 aatgattctc aatatatatt acgctctcta cgcagaaaaa acaagcgatt tagagaaaaa
      781 cacacatgcg aatgacagcc ataaaactac aaccagtggt actcacaaca caacagtaga
      841 tagcgcaacg caagaaaaag aatatatcga aaaactcaac cgtcgccaca aagtggtcct
      901 ctccgcgcag cgtagcgaaa gccgcctcgc cgactcggtg gtgcaagtcg aggttattga
      961 caaagagcaa atccgtgaga aaggcgcgcg caccgtcacc gaggcactca acaatgaaac
     1021 cggttttttt atccaacgcg gtattttcgg cgataacgtg caactacaag gtctcgattc
     1081 gcaatacttg ttgattatga aagacggcga acgtctgaat gggcgtatca acgggcaata
     1141 cgacaccagc cgcttgcgtg tcgacaacgt cgaacaaatt gaaattgtgc gcggctcttc
     1201 gtcggcaatt tacggatccg acgctatcgc tggagtagtg aatatcatca ccgcccgccc
     1261 gaaaaaacca ctcatcgatg ctaaactaca gggaggcaat ttaggcgcag ccgatctttc
     1321 gggttcggcg gtaatcccac tgggggattt tctcgtgcgc ggctcgggag tgt
//
LOCUS       DAOMPD010000639         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_34872_length_1369_cov_2.277778, whole genome shotgun sequence.
ACCESSION   DAOMPD010000639 DAOMPD010000000
VERSION     DAOMPD010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34872_length_1369_cov_2.277778"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..433
                     /gene="acpP"
                     /locus_tag="PLY93_14990"
     CDS             191..433
                     /gene="acpP"
                     /locus_tag="PLY93_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRP70829.1"
                     /translation="MASTEEIMGKVKKIISEQLGVDEGEVTNESHFIDDLGADSLDTV
                     ELVMALEEEFGIEIPDEDAEKIQTVGDVQKYIEEHS"
     gene            complement(435..1091)
                     /locus_tag="PLY93_14995"
     CDS             complement(435..1091)
                     /locus_tag="PLY93_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRP70830.1"
                     /translation="MSNDYLTDRRRARIANVLENRTRRVTVLLEDIYDPLNANAVLRT
                     CDGLGIQDIHVAENRHKFTVHSKVSGHTAHWLTLHRYRTHSLPAKTDFRIKPEGVQPS
                     TEKAYKHLKDAGYIILATSPRGTPTPLDNLDTTKKYVVALGSEHWGLSDYAIENGDKT
                     IALPMLGFTESYNISVTAAMILFYLTKRLTGERGLTEHEKNQLWQQWGARGAKPLEPT
                     "
BASE COUNT          365 a          310 c          337 g          357 t
ORIGIN      
        1 gcagccatag tttcgagatt cgcataaatg gttttttgat agtatgaaaa ttatggcaaa
       61 aaaataaact gggcaatacc tcgcacggga tttttagggg gacaaggcgc accgcgccgc
      121 gcgcccttaa agaaacattt gcccctttgg ggtaaaacgt gtagcggttt tagccccaaa
      181 ggagacccaa atggcttcaa cagaagaaat catgggcaaa gtaaaaaaaa tcatctctga
      241 gcaactcgga gttgacgaag gcgaagttac taacgagagc cacttcatcg acgatctcgg
      301 tgcagactca ctcgacactg tcgaactcgt tatggcactc gaagaggaat tcggtatcga
      361 aatccccgac gaagacgctg aaaaaatcca gactgtcggc gacgtacaaa agtacatcga
      421 agaacattct tgatttatgt gggttcgagg ggcttcgccc ctcgagcgcc ccattgctgc
      481 cataattgat tcttttcgtg ttctgttaaa ccgcgctcgc cggttaatct ctttgttaaa
      541 taaaataaaa tcatcgccgc cgtcactgat atattatagc tctctgtaaa tcccaacatc
      601 ggtagcgcaa tcgttttgtc tccattttca atcgcatagt cggatagccc ccaatgctcg
      661 ctccccagcg ccactacgta tttttttgtc gtgtcaaggt tgtcgagagg agtgggggtg
      721 cctcgtggcg aggttgcaag aattatataa ccggcgtctt tcaagtgctt gtatgctttt
      781 tccgtgctcg gctgcacccc ttcaggcttt atgcggaaat cagtttttgc cggtaatgag
      841 tgggtgcggt aacggtggag agtcagccaa tgcgcggtgt gtcccgacac ttttgagtgg
      901 accgtgaatt tgtggcgatt ttcagcgacg tgaatatctt gaataccgag gccgtcgcaa
      961 gtgcgcagta ccgcgttggc gttaaggggg tcgtaaatat cttcgaggag cacggtgacg
     1021 cgccgcgtgc ggttttcgag tacatttgcg atgcgcgctc tgcggcggtc ggtcagataa
     1081 tcgttcgaca tggagagata cgaatatata aaaaaacagt ttcaacgaca ataacaaaat
     1141 taagggggct gtgcgccaac tcgtgtagcg ctcatcaaga agcaattttt tacaaaaaaa
     1201 ttgccatttt atatacaccg cgcgagttgg cgtacaagct cctcattaat tcctttgcgc
     1261 cttgtttcac attgtgtctg tgccgtatgc catggatgtt agaaaagcgt gagtttgatg
     1321 caccacaacg attaaaagca cttctcaaag aaaataaaat catacaaat
//
LOCUS       DAOMPD010000640         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35005_length_1365_cov_2.130534, whole genome shotgun sequence.
ACCESSION   DAOMPD010000640 DAOMPD010000000
VERSION     DAOMPD010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35005_length_1365_cov_2.130534"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLY93_15000"
     CDS             complement(<1..1158)
                     /locus_tag="PLY93_15000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRP70831.1"
                     /translation="MSNSTLSYRNDLQGLRALAVIAVLFFHIWPDSYRGGFIGVDVFF
                     VISGFLITSLLILEAEKKQRIDLANFWSRRMRRLLPAATLVLLISLFLAFFYMPESDW
                     LNTAKQVFASSLYFQNWMLVAQSVDYMAREASATVVQHYWSLSIEEQFYIAWPLFIAL
                     AAAISRSTKRRVRNVALVFSIVLLISSFCISAFVYLGAPHGYFTSTTRVWELSLGATL
                     ALLWPYIQWKNVTRVILSWMGFILVVLSIFVIKQDQNFPGYIALLPTVGAILLILGNQ
                     VRGAAGTLLGYQPLRYIGDISYAVYLWHWPLVVLAKQAPEIQQLPSWQQSGGIIVATL
                     TLSVLTKYLVEDPFRHGFLAFGLRSQKRTLFVTSSIVSAVVLVATSSSIALF"
     gene            1256..1328
                     /locus_tag="PLY93_15005"
     tRNA            1256..1328
                     /locus_tag="PLY93_15005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1289..1291,aa:Met,seq:cat)
BASE COUNT          421 a          322 c          349 g          273 t
ORIGIN      
        1 gaaaagtgca atgcttgaac tggtcgcgac caacacaacg gcgctcacga tggagcttgt
       61 aacaaagagg gttcgctttt gggaacgcag cccaaacgcc aaaaaaccat gcctaaacgg
      121 atcttcgaca aggtatttcg tcaacaccga gagggtcagc gttgccacaa tgatgccacc
      181 gctctgctgc catgaaggaa gctgttggat ctcgggcgct tgtttggcaa gaactaccaa
      241 cggccagtgc catagataca ctgcgtacga aatatcaccg atgtaacgca atggttgata
      301 acccaagaga gtacccgccg cgccacgcac ttggttacct aaaatgagga ggatagcgcc
      361 gaccgttggc aaaagagcga tatacccggg aaagttttgg tcttgcttga ttacgaaaat
      421 tgaaagcaca acaagaataa atcccatcca gcttaatatg acacgcgtta catttttcca
      481 ttgtatataa ggccacaaaa gcgcaagcgt tgcccccaac gaaagctccc acacacgggt
      541 ggtgctggta aagtagccat gtggtgcgcc caaatacaca aacgcggaaa tacaaaaaga
      601 agagatgagc agtactatcg agaaaacgag agcgacattg cgcacacgcc gctttgtgct
      661 acgactgata gcggcggcaa gtgcgataaa aagtggccat gcaatataga actgctcttc
      721 aatcgaaagc gaccaatagt gctgtaccac ggttgcgctt gcctcgcgtg ccatgtagtc
      781 gaccgactgc gcgacaagca tccagttttg aaagtagaga gaggaagcaa aaacttgctt
      841 tgcggtgttg agccaatcgc tttcgggcat ataaaagaac gctaaaaaaa gagaaatgag
      901 gagcacgagt gtcgccgccg gaagaaggcg tcgcatacga cgcgaccaaa aatttgctaa
      961 gtcgatgcgt tgtttttttt ccgcttcgag aattaaaagg ctggtgatga gaaaacctga
     1021 aatgacgaaa aacacatcga caccgataaa tccaccgcgg taactatccg gccaaatatg
     1081 aaaaaaaagc acagcgataa ccgcaagtgc gcgcaaacct tgtaggtcgt tacggtacga
     1141 taaagtggag ttgctcatgt aaaattaacc gtgaagggct atttaagaga atgcgttgac
     1201 aagtgaagct aaaaagagtt gtcacgctgt ctcaagaagc aattttgcct tacaccgcgc
     1261 ggtagagcag ttggtagctc gttgggctca taacccaaag gtcgcaggtt caagtcctgc
     1321 ccgcgctaca cgaaaggggt gccccccaca ctcaagtgtg ggggg
//
LOCUS       DAOMPD010000641         1364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35032_length_1364_cov_2.653170, whole genome shotgun sequence.
ACCESSION   DAOMPD010000641 DAOMPD010000000
VERSION     DAOMPD010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35032_length_1364_cov_2.653170"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLY93_15010"
     CDS             <1..187
                     /locus_tag="PLY93_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70832.1"
                     /translation="PQRYYRRKPVYRIHGMEVTWWNFSTTTSRSINLFSDGTISENYL
                     LTETPLSVFSLRRAPNS"
     gene            249..1343
                     /gene="obgE"
                     /locus_tag="PLY93_15015"
     CDS             249..1343
                     /gene="obgE"
                     /locus_tag="PLY93_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRP70833.1"
                     /translation="MHQFVDEMIIRVQAGKGGAGEISFLREKFMEFGGPDGGDGGDGG
                     DVIFTTSEQLLALSHLRPAHLYKARNGFPGEGSNCNGKKGADLIIRVPYGTQVIDPTT
                     HALLHDFTEEGTYTAAQGGRGGKGNAFFKTARRQAPRLAQPGEETVEKEFLLSLKMIA
                     DVGFVGFPNAGKSTLLKALTKANPKIANYPFTTLSPNIGVIDDLAPRPLIVADIPGIL
                     EGASKGYGLGLSFLKHIERVRLIVFVIDLENAFVEDELKILKNEIESYSPELLLKPSI
                     LVLNKIDRFEDKKFLKAFIASYKKQGLNPIPISAHTGEGMDKLRSELVKLLKLRAQKK
                     AVKKKSQKKKAIKTIKKTAKKKSSVKKVPK"
BASE COUNT          396 a          318 c          316 g          334 t
ORIGIN      
        1 cccacagcgc tactaccgta gaaagccggt ttataggatt catggtatgg aagtgacgtg
       61 gtggaacttt tccacaacaa caagccggag tatcaacctt ttttccgacg gcacgattag
      121 tgaaaattat cttttgacag aaacgccatt atccgttttt agtttacgcc gcgcaccaaa
      181 ttcatagtct atcagccctg taacgtacac agatttcaaa atctgctgca cgtaccagtg
      241 aggttccttt gcatcaattt gtcgatgaaa tgattatccg tgttcaagcg ggcaaagggg
      301 gcgcgggtga aatttctttc ttacgcgaga aatttatgga gtttggaggc cccgacggtg
      361 gagacggcgg cgacggtggc gatgtaattt ttaccacctc cgaacagtta ctcgcgcttt
      421 cgcatttgcg ccctgcgcat ctttacaaag cgcgcaacgg ctttccgggc gaaggttcaa
      481 actgcaacgg gaagaaaggg gcggatctca taatccgtgt gccctatggc acccaagtga
      541 tcgatcccac tacacacgcc ctcttgcacg acttcaccga agaaggaacg tacaccgctg
      601 cgcaaggcgg acgcggcggc aaagggaacg cttttttcaa aactgcgcgt cggcaagcgc
      661 cacgcctcgc acagccgggc gaagaaacag ttgaaaaaga atttttactt tcactgaaaa
      721 tgattgccga cgtgggcttt gtaggctttc ctaatgcggg taaatcgacg ctgttgaaag
      781 cgctcaccaa agccaaccca aaaattgcaa attatccttt cacaacgctt tctcccaaca
      841 tcggcgtcat cgacgattta gctccgcgcc cgcttatcgt ggctgacatc ccggggattc
      901 tcgaaggggc atccaaaggg tatggcttgg gactctcctt tttgaaacat atcgagcgcg
      961 tccgattgat tgtcttcgtg attgatttag aaaatgcgtt tgtcgaagac gagctcaaaa
     1021 ttctcaaaaa tgaaattgaa tcgtatagtc ccgagctgct cttgaaacca tcgattttag
     1081 tcctcaataa aattgaccgc tttgaggata agaaattttt aaaagcgttt atcgcatcgt
     1141 ataaaaaaca aggccttaac cccataccca tttcagcgca caccggcgag ggcatggata
     1201 aattgcgtag cgagttggtg aaattgttaa aattgagagc gcaaaagaaa gcggtaaaga
     1261 aaaaatccca gaaaaaaaag gcaataaaga caataaagaa gactgcaaaa aagaaatcat
     1321 cggtgaaaaa agttccgaaa taaatccagt tatttctttt tttt
//
LOCUS       DAOMPD010000642         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35170_length_1360_cov_2.278927, whole genome shotgun sequence.
ACCESSION   DAOMPD010000642 DAOMPD010000000
VERSION     DAOMPD010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35170_length_1360_cov_2.278927"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>218)
                     /locus_tag="PLY93_15020"
     CDS             complement(<1..>218)
                     /locus_tag="PLY93_15020"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70834.1"
                     /translation="NVTHPVCALSTPSGRSAIAVIRATGTGVFNALEHFFHPKAKRGV
                     HALDARTTYYGTLREGGEFIDDVLLIKF"
     assembly_gap    219..244
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<245..992)
                     /gene="jag"
                     /locus_tag="PLY93_15025"
     CDS             complement(<245..992)
                     /gene="jag"
                     /locus_tag="PLY93_15025"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRP70835.1"
                     /translation="MQILEIEALDEKEALKKGIEYLGVDAESIELSVLKKGSSGFLGL
                     GQKTAGLYKISAIRGKTPLAVIIRGVISTILGHIGYTVTVKDYTKVEEGKLMVTLERE
                     YAGYIIGKHGRTLEALQFMTNLIVEKITGEQPKILLDIENYRERRAQHLMEIARKTGE
                     YVLRTGKSRLLDPLNPYERRLVHMALQDNEHIKTESEGNGVYKRVRIFRVSSGAPAET
                     SNELVTEENIGNIQFEAGEDPEGAEDIEFKD"
     gene            complement(1025..>1360)
                     /locus_tag="PLY93_15030"
     CDS             complement(1025..>1360)
                     /locus_tag="PLY93_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRP70836.1"
                     /translation="WKSPFIPGWIPDLSQPDYFFSLPATWPYIGGFAIHLLPIIMTFS
                     QYFQTKLTPTSGDANQQKIMQFMPFMMLFLFYKMPAGVVLYWTVQNALSIVQQVYTNR
                     KTKANAASA"
BASE COUNT          321 a          317 c          327 g          369 t
ORIGIN      
        1 gaatttaata agaagtacat cgtcgatgaa ctcgccaccc tcgcgtaaag tgccgtagta
       61 ggtagtgcgc gcatcgagtg cgtgcacgcc gcgtttggct ttggggtgaa aaaaatgttc
      121 cagtgcgtta aagacacccg tccccgtcgc gcgaataacg gcaatcgccg accgaccgga
      181 aggggtggaa agcgcacaga cggggtgcgt cacattctnn nnnnnnnnnn nnnnnnnnnn
      241 nnnncgtctt tgaactcgat atcctctgcc ccctcgggat cttcacccgc ttcaaattgg
      301 atgttaccga tattttcttc ggtgacaagt tcgttcgaag tttcagcagg tgcaccgctg
      361 gaaacacgga aaatacgcac acgcttataa acgccgttcc cctcggactc ggttttgatg
      421 tgttcgttgt cttggagggc catgtggact aaacgccgct cgtacgggtt caaaggatct
      481 aagaggcggc ttttgccggt acgtaagaca tactcgcctg ttttacgcgc aatttccatg
      541 aggtgttgcg cgcggcgctc gcggtagttc tcaatgtcga gtaagatttt gggttgctcg
      601 cccgtgatct tctcgacgat gaggtttgtc ataaactgca acgcttcaag ggtgcgacca
      661 tgcttaccaa taatatagcc cgcgtattcg cgttctaaag tcaccatgag tttaccttct
      721 tcgaccttgg tgtaatcttt tacggttacg gtgtaaccga tatggcctaa aattgtcgaa
      781 atgacaccac ggatgattac cgccagcggt gttttaccac ggatagcgct aattttgtag
      841 agccccgccg ttttttgccc taagcccaaa aaacccgaac tccctttttt taatacactt
      901 agttcgatcg actccgcgtc gacccccaaa tactctatac ctttttttaa cgcttctttt
      961 tcgtctaagg cttcaatttc taaaatttgc atatatcctc gctttatttc ctaatatgag
     1021 ttcgttatgc gctcgcagca tttgctttgg tctttcgatt ggtatagacc tgttgcacaa
     1081 tggaaagtgc gttttggaca gtccagtaaa gcaccacacc ggcaggcatt ttgtaaaaca
     1141 agaagagcat cataaacggc ataaactgca taattttctg ctggttcgca tcgcccgagg
     1201 tgggtgtgag tttagtttga aaatactgcg agaatgtcat gataatgggt aataggtgga
     1261 tggcgaaacc gccaatataa ggccatgttg cgggtagtga aaagaagtaa tcgggttgcg
     1321 acaagtcggg tatccagccg ggtataaaag gcgatttcca
//
LOCUS       DAOMPD010000643         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35361_length_1354_cov_2.077752, whole genome shotgun sequence.
ACCESSION   DAOMPD010000643 DAOMPD010000000
VERSION     DAOMPD010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35361_length_1354_cov_2.077752"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLY93_15035"
     CDS             <1..564
                     /locus_tag="PLY93_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70837.1"
                     /translation="KASTDLAVNAFTALLGNIGGAALSFLIAFFLVSSVSAEVMIGPR
                     VYYAMAEQGLLFKKLAQVQPRLGTPAFAILLQSALSVVYILTFGFGGTLKLLIFMGFA
                     LSFFPWLTVFGLLYAHFFKRIAVIANRPYRSPLFPLFPVFYLLASGFMMVSSLIYSAR
                     ESLWAIGITLTGAVCFFVWQWYMKRQS"
     gene            691..>1354
                     /locus_tag="PLY93_15040"
     CDS             691..>1354
                     /locus_tag="PLY93_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRP70838.1"
                     /translation="MSDAPHPPVRPPYSRFKGFLFLTVFIFAIIAGIVYIDSRKAMES
                     APLAKVATEKATATKNASVVQGEIPLDWRNDLGVPVLCYHQIVTEAQYREKPTPFAVT
                     AKEFAAQMDWLYEKKFYTILPDDLVAYTRGEKNLDFNNGKRPIVLTFDDGNNDFIEHA
                     LQPMNKYGYKGVLFIYPTYIMAKPHKKRALTWEQIRNLGKAGHAIESHTMWHPNLSTM
                     SDD"
BASE COUNT          307 a          321 c          317 g          409 t
ORIGIN      
        1 aaagccagca ccgatttagc ggtgaacgca ttcactgcgc tcttggggaa tatcggcggt
       61 gccgcgcttt catttttgat tgctttcttt ttagtgtcgt cggtttccgc cgaggtaatg
      121 attggcccgc gcgtgtatta tgcaatggcc gagcaggggc ttcttttcaa aaaacttgca
      181 caggtgcaac cgcgcttggg tacccccgca tttgcaatcc ttcttcaatc ggcgttgagt
      241 gtcgtgtata tcctcacgtt tggttttggc ggcacgttga aacttttaat tttcatgggg
      301 tttgcgctga gttttttccc ttggcttacc gtctttggtt tactctacgc acatttcttt
      361 aagcgcatcg ccgtcattgc aaaccgacct taccgctcac cgctttttcc gttgtttccc
      421 gttttttatt tattggcatc ggggttcatg atggtgtcgt cgcttattta tagcgctcgc
      481 gaatctttat gggcgattgg gattactctc actggagcgg tttgtttctt tgtatggcaa
      541 tggtatatga aacggcagtc ctgaaagcct gtgcgttaac tcgctgagac aatttttttg
      601 taaatttttg tgcacgctgc gcgagttggc tcacaggctt tttcgtacac aaactcctgt
      661 gcgcgctgtc tcaccatttc tttatgtcac atgtctgatg ctccgcaccc accagtgcgc
      721 ccaccgtatt cgcgtttcaa gggatttctt tttttaacag tttttatttt tgctattatt
      781 gcaggtatcg tttacatcga tagccgtaaa gcgatggaaa gcgctcccct tgcaaaagtt
      841 gctactgaaa aagcaactgc gacaaagaat gcctccgtcg ttcaagggga aatccccttg
      901 gactggcgca acgacttggg ggtgcccgtt ttatgctacc accaaattgt caccgaagcg
      961 caataccgcg aaaaacccac accgtttgct gtaacagcaa aagagtttgc cgcgcaaatg
     1021 gattggctct acgagaagaa attttatact attttaccag acgaccttgt tgcgtacacg
     1081 cgcggtgaga agaatctaga ttttaataat ggtaaacggc cgattgtgct cacattcgac
     1141 gatggcaaca acgattttat cgaacacgcc ttacaaccga tgaataagta tggctataaa
     1201 ggtgtgcttt ttatttatcc cacatacatt atggcgaagc cgcataaaaa gcgcgcttta
     1261 acatgggagc aaatccgtaa cttgggcaag gctggccatg cgatcgaaag ccacacgatg
     1321 tggcatccca atttgagcac gatgagcgac gatg
//
LOCUS       DAOMPD010000644         1349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35501_length_1349_cov_3.376352, whole genome shotgun sequence.
ACCESSION   DAOMPD010000644 DAOMPD010000000
VERSION     DAOMPD010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35501_length_1349_cov_3.376352"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLY93_15045"
     CDS             complement(<1..1241)
                     /locus_tag="PLY93_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRP70839.1"
                     /translation="MLQWTQSSIFIRLRLAAALVLVLYTATHFLNHALGLFSLDALEE
                     GRKYFLLFWRNSVLQFVFPTALLLHVSSVLLRLMFHQNWNFTTREKVKIFSGLLIPFF
                     LLLHIASTRLEWWIYGYNDTYSYYLYYNFRGLGIYLLLAMTLLIWTHAYLGLTDILKL
                     KKWYEKYRTTFGIAFVVTPLLGLSGLIAAAGEVRRLAVDNAWLAKTALAHGNPANAAR
                     TQYQLYAILIGCLVLLLICLYLIRVFVVLRLVRQATSSVTYADGRQVRVAPTTTLLDA
                     SLINHIEHAHVCGGNGRCSTCRVRVLEGAENLSLADARETALLKRVGAESDVRLACQA
                     KVSGNVKIALLIPPHKATPAGAAALLKPHTKSDGMEKEVVVFFSDLKGFTSFSEHKLP
                     YDVVFVLNQYFRSMGKIIEDNG"
BASE COUNT          379 a          318 c          350 g          302 t
ORIGIN      
        1 ccgttatctt cgataatttt gcccatgctg cggaaatatt ggttgagtac aaaaaccacg
       61 tcgtaaggca acttatgctc ggaaaacgaa gtgaatcctt ttaaatcgga aaaaaatacg
      121 acgacttctt tttccatacc atcgctttta gtatgtggct tcaggagcgc agcggcgccg
      181 gcaggcgtgg ctttatgcgg tggaataagg agcgcaattt taacattccc cgaaactttg
      241 gcttggcacg cgaggcgcac atcgctttcg gcgccgacgc gcttcaagag cgctgtttca
      301 cgcgcatcgg caagcgataa gttttctgca ccttcgagta cgcgcactcg gcatgtcgaa
      361 cagcggccat tgccgccgca aacatgcgcg tgctcgatgt ggttgatgag gcttgcatcg
      421 aggagcgttg tcgtcggcgc aacccgaact tgccttccgt cggcgtaggt gacgctgctc
      481 gtcgcttgac gcaccaaacg cagcaccaca aacacccgaa tcagataaag gcaaatcagc
      541 aataaaacca gacagccaat gagaattgca tagagttggt attgagtgcg tgccgcattt
      601 gcggggttgc cgtgcgcgag tgcagtcttt gcaagccatg cgttatcgac cgccaaacgc
      661 ctcacttcac cagcagcggc gattaatccc gaaagaccta aaaggggggt cactacaaaa
      721 gcgataccaa acgttgtgcg gtatttctcg taccattttt tgagttttag tatatcggtg
      781 agtcctaagt acgcgtgcgt ccatattaac agcgtcatcg ctaagagaag gtaaataccc
      841 aaaccacgga agttgtagta taaatagtaa gaataggtat cattataacc gtatatccac
      901 cattcgagac gggtgctcgc gatatggagc aataaaaaaa acggaattaa taagccgcta
      961 aaaattttaa ctttctcgcg cgtggtgaaa ttccagtttt ggtggaacat gaggcgcaag
     1021 agcacgctcg acacatgcaa gagcaatgca gtggggaata caaactgcag aacgctattg
     1081 cgccaaaaca ggagaaagta ttttcggcct tcttcaaggg cgtcgagaga gaaaaggccg
     1141 agcgcgtggt tcaaaaaatg cgttgcggtg tagagaacca gcaccagcgc agcggcaagg
     1201 cgcagccgaa taaagataga cgattgagtc cactgaagca ttgcgcaaga tttacgaaac
     1261 gagtctgtgc gccaactgca aattgacgga acgatatgca acccacagag ataactgaca
     1321 cttttggggt caatttgagt gcgttttag
//
LOCUS       DAOMPD010000645         1347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35569_length_1347_cov_2.765480, whole genome shotgun sequence.
ACCESSION   DAOMPD010000645 DAOMPD010000000
VERSION     DAOMPD010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35569_length_1347_cov_2.765480"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLY93_15050"
     CDS             <1..207
                     /locus_tag="PLY93_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70840.1"
                     /translation="LFMIVAGNDFLADIKVTEALAHQIDSSLLRYDFYPENYHENFNE
                     LNRNKIFKDIEKWVTTQLKKNLKS"
     gene            complement(231..1049)
                     /gene="lpxI"
                     /locus_tag="PLY93_15055"
     CDS             complement(231..1049)
                     /gene="lpxI"
                     /locus_tag="PLY93_15055"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRP70841.1"
                     /translation="MILNNLALFAGAGKLPAIAVKNARQKGISPIVYHIAETTLDPTL
                     KNDAMLKVRSVSIGEMVKTFQFLQEDKITHIVLLGKFEKQRLLQDVERDATAERLYAA
                     APDRRDDTLFHEFARLVAQAGIQIYEQKLFLDGCFLPQGVHTRLKPELPTLLADIEFG
                     YSLSQRVGELDIGQTAIVYEKMILAVEAIEGTDAAISRAGAIVRKRGGVVCKSAKASQ
                     DARFDLPAVGIRTLETMAESGMQALVIEAEQTLVIDPKEFIAKADALGLIVVAQ"
     gene            complement(1057..>1347)
                     /locus_tag="PLY93_15060"
     CDS             complement(1057..>1347)
                     /locus_tag="PLY93_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRP70842.1"
                     /translation="KAAVNATMPVTLVMLECIRDYYDDTGVKIGMKPAGGIRTSKEAL
                     HYLVMVKETLGDAWLSPDWFRFGASTLLNDLLMQYAKQVKGGYQSLDYFSLS"
BASE COUNT          350 a          317 c          330 g          350 t
ORIGIN      
        1 ctttttatga ttgttgccgg caacgacttt ttggcggata taaaagtaac agaggctttg
       61 gcgcatcaaa tcgattcatc gcttctacgc tacgattttt atcctgaaaa ttaccacgaa
      121 aatttcaacg agctcaatcg aaacaaaatt tttaaagata ttgaaaaatg ggttacgaca
      181 caactgaaaa aaaatctcaa aagttaaggt agccatcctt cgctgtatag ttactgcgct
      241 acgacgataa gccctaatgc atcggccttg gcaataaact ctttcggatc gataacaagg
      301 gtttgttcgg cttcgatgac gagtgcttgc atgccggatt cggccatggt ttccagagtg
      361 cgtattccta ccgcgggtaa gtcgaaacgc gcatcttgcg aagcttttgc acttttgcaa
      421 acgacaccgc cacgcttgcg cacaatcgca cccgcacggc taattgcggc atcggtgcct
      481 tcgattgctt cgacggctaa aatcattttt tcataaacga tagcggtttg gccaatgtcg
      541 agttcgccca cgcgctgact caagctataa ccaaactcga tgtcggctaa gagagtgggc
      601 aactcgggct tgagccgtgt atgcacacct tggggcaaaa agcagccgtc taaaaaaagt
      661 ttttgttcat aaatttggat gcccgcttgc gcgacaaggc gtgcgaattc gtgaaagagc
      721 gtatcgtcgc ggcgatcggg cgcagcggcg tagaggcgtt cggcggtggc gtcacgttcg
      781 acatcttgca agaggcgctg tttttcaaac ttgcccaaaa gcacgatgtg cgtaatttta
      841 tcttcttgta aaaactggaa tgttttcacc atctctccaa tggaaacaga ccgcactttg
      901 agcatagcgt cgtttttcaa tgtaggatcg agtgttgtct cggcaatatg gtacacgata
      961 ggggaaatcc ccttttggcg tgcattcttg actgcaatcg caggcaattt acctgcgccc
     1021 gcgaagagag caagattatt gagtatcact gaaaaattaa ctcaatgaaa aataatcgag
     1081 tgattggtag ccacccttta cttgcttggc gtactgcatg agcaaatcgt tgagtagcgt
     1141 cgatgcgccg aaacgaaacc aatcgggaga aagccacgcg tcgcccaacg tttctttgac
     1201 catcaccaga taatggagcg cttctttcga ggtgcggatg ccaccggcgg gtttcatgcc
     1261 aattttgacg cctgtgtcgt cgtaataatc gcggatgcat tcaagcatga caagagtgac
     1321 tggcatggtg gcgttcaccg ccgcttt
//
LOCUS       DAOMPD010000646         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35608_length_1346_cov_2.367157, whole genome shotgun sequence.
ACCESSION   DAOMPD010000646 DAOMPD010000000
VERSION     DAOMPD010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35608_length_1346_cov_2.367157"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..689
                     /locus_tag="PLY93_15065"
     CDS             246..689
                     /locus_tag="PLY93_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70843.1"
                     /translation="MKYGFLFLFFALSPLLAEEIYLTCTVTQIAASQKEGQIDTKLKK
                     MEKFIQKDDSLSQFSSFKYLGKKTLNATKEKLASMKLKNGQALKMKPVSVLRAHRKNT
                     ITLDLRIGNEELKKRIIDRNSLFVRAGSLPKSAELVLAFSCPVFP"
     gene            693..>1346
                     /gene="guaA"
                     /locus_tag="PLY93_15070"
     CDS             693..>1346
                     /gene="guaA"
                     /locus_tag="PLY93_15070"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRP70844.1"
                     /translation="MKKKSPPTQKQTQKAAPPAQKIIVLDFGSQYTQLIARRLRELRF
                     YTEIHPYHIGIEAINAQKPAGIILSGGPSSVYDKDAPMVDKKVFEMGIPILGICYGLQ
                     LICHLMGGKVAPSTEREYGRASLRAKSKSPLLQGIKLGRRKEGTPARANSVTSVSPEQ
                     VWMSHGDRIHKLPKNFSVIGVTENSPMAAFADEKNKIYGVQFHPEVHHSTSGKTILKN
                     "
BASE COUNT          378 a          313 c          301 g          354 t
ORIGIN      
        1 agatagcgta cgagggttgg tcgtatggtc cttcgatggg gcttatgtcg aatctctttg
       61 gccgcgtcta ttggggggtt tcagtggggt attatacttt tgtcagcggt ggctacaaag
      121 cgagcgagtg gagttggtca cccgcaattt ttatccctct ttgatttttg cttattttga
      181 tataggccaa accgcatagt tctctttctg ctttatcgcc tatttcatgg attgaattat
      241 gtcgcatgaa gtatggattt ctttttctat tctttgcgct ttcccctctt ttggccgagg
      301 agatttattt aacgtgtact gtcacacaaa ttgctgcgtc tcaaaaagaa ggtcaaatcg
      361 atacaaaatt aaaaaaaatg gaaaagttta tccagaaaga cgatagcctc tcccagtttt
      421 cgtcgtttaa gtatctcggt aaaaaaacat taaacgcaac gaaggaaaaa ttggcctcga
      481 tgaaactaaa aaacgggcaa gcattaaaaa tgaaacccgt ttcagtatta cgcgcacacc
      541 gaaaaaatac aatcaccctt gaccttcgca tcggaaatga agaactgaaa aaaagaatta
      601 tcgaccgcaa ctcccttttt gtacgcgcag gttctttgcc taaatcggca gagctcgtgc
      661 tggcgtttag ttgtcccgtg tttccgtaaa gtatgaaaaa gaaatccccc cccactcaaa
      721 aacaaacgca gaaagcggcg cctcccgcac aaaaaattat tgtgctcgac tttggatcgc
      781 agtacacaca gctcatcgca cgccgtctac gcgagctgcg cttttatacc gaaattcacc
      841 cgtatcatat cggcatcgaa gcaatcaatg cacaaaaacc ggcggggatt attctgtccg
      901 gtgggccttc ctcggtgtac gataaggatg cacccatggt ggataaaaaa gtttttgaga
      961 tgggaattcc aattttggga atttgttatg gtctccaact catttgccat ctcatgggag
     1021 ggaaagtcgc tccctctacc gagagagaat atggccgcgc gtcactacgc gcaaaaagta
     1081 agtcgccgct tcttcaggga attaagctgg gtcgtcgcaa agagggcact cctgcgcgcg
     1141 ctaactcggt aacttcggtg tcaccggaac aagtgtggat gtcgcacggc gatcgcatcc
     1201 acaagctgcc aaagaatttt tctgtaattg gcgtgaccga aaactcgccc atggcggcgt
     1261 ttgccgacga aaaaaataaa atctacggcg tgcaattcca ccccgaagta caccactcga
     1321 catcaggaaa aactattctt aaaaat
//
LOCUS       DAOMPD010000647         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35696_length_1343_cov_2.827640, whole genome shotgun sequence.
ACCESSION   DAOMPD010000647 DAOMPD010000000
VERSION     DAOMPD010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35696_length_1343_cov_2.827640"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1036)
                     /locus_tag="PLY93_15075"
     CDS             complement(293..1036)
                     /locus_tag="PLY93_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRP70845.1"
                     /translation="MVLTTFESFIYRITLNRPEKHNAFDADMIEAVTSAVQATPSEAR
                     VIFFTGNGKSFCAGADINYMRAQALFSEARNRDDAKKLSQMFEAIYEQTLPTVVYAHG
                     NIFGGGLGILAACDIAFAADDAKFCFSEVKLGIIPAVISPFVLRKTGVAFARRYFQSA
                     EVFNSEVAQKVGLISEQLPSALAEEKTAQLLSQLVAAGSDAQRSVKKLLHAYETFEPL
                     SRDQLIFELVKLRSSNDAQERMQRFLEKK"
     gene            complement(1038..>1343)
                     /locus_tag="PLY93_15080"
     CDS             complement(1038..>1343)
                     /locus_tag="PLY93_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRP70846.1"
                     /translation="SVMGGEQAAGVLNIVREQAAAAKAKKTGVPVDKEKLAMENVETK
                     ARLTAQFEKQSDAFYSSARLWDDGVIHPVETRSALILGLLAAASRPLSRTRQPVYRM"
BASE COUNT          359 a          328 c          292 g          364 t
ORIGIN      
        1 tcaaaaaaat tagatttttt gaggaaaatt atcagttagg atagttatcc ctaaatgtga
       61 cataagccga aaaacaggaa tagttacccc ctgatgcgac ataagcctaa aaccggtata
      121 gttaccccct aatgtgacat aagcctaaaa taatgatacg tttatacgaa ctcatcgtgc
      181 attgaataag ggagcgcaga taaatttctt tctatttata tttaccgatg gcgctaacat
      241 cgaagagcgt tggttccctt gcccaaccaa cgtccgtcac aaacacatca aattatttct
      301 tttctaaaaa acgctgcatc ctctcttgcg catcgttgct ggagcgtagc ttcaccaatt
      361 caaaaataag ttgatcacgg cttaagggtt caaaagtttc gtaggcatgt agaagttttt
      421 ttacgctacg ctgtgcgtcg ctgcctgctg cgactaactg cgacaaaagc tgcgctgttt
      481 tctcttcagc taaagcggat ggtaactgtt ccgaaatcaa tcccactttt tgagcgactt
      541 cagagttaaa aacttctgcg ctttgaaaat agcggcgcgc aaacgccaca ccggtttttc
      601 tgagcacaaa cggtgagatc accgccggta taatgccaag cttcacctct gagaaacaaa
      661 atttagcgtc atcggcggca aaggcgatat cgcacgccgc taaaatcccc aaaccaccgc
      721 caaaaatatt tccatgcgca tagacaactg tgggtaatgt ttgttcgtaa atcgcctcga
      781 acatttgcga gagttttttg gcgtcgtcgc ggttgcgcgc ctctgaaaaa agagcctgtg
      841 cgcgcatgta attgatgtcc gcacccgcgc aaaaagattt accattacca gtgaaaaaaa
      901 tgacgcgtgc ttcgctggga gtcgcctgta ctgcgctcgt caccgcttcg atcatatcgg
      961 cgtcgaatgc gttgtgcttt tcgggacgat ttaaggtaat acgataaata aaactctcaa
     1021 atgttgttaa gaccatttta catcctataa acgggttgtc ttgttcgtga aagggggcgc
     1081 gacgctgccg ccaaaagccc cagaattagc gcactgcgtg tttctacagg atgaatcaca
     1141 ccgtcatccc atagccgtgc ggaggaataa aacgcatcgc tttgcttttc aaattgcgcg
     1201 gtgaggcgtg ctttggtttc aacattttcc atggcaagct tttctttatc cactggcacg
     1261 cctgtctttt ttgctttcgc tgcggcggct tgctcgcgga cgatgttcaa tacacccgct
     1321 gcttgttcac cgcccatcac gga
//
LOCUS       DAOMPD010000648         1342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35730_length_1342_cov_2.761461, whole genome shotgun sequence.
ACCESSION   DAOMPD010000648 DAOMPD010000000
VERSION     DAOMPD010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35730_length_1342_cov_2.761461"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLY93_15085"
     CDS             complement(<1..336)
                     /locus_tag="PLY93_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70847.1"
                     /translation="MTIAVHTQPTFWGVAIQINAETKFPSIAIREIYRRLASAEDQFM
                     LESITPDIFPGDNLSEETIDTQFKYLIRLTCAGDKASVKQTLAKILKGASGIAKIGLG
                     LIENKTTESQ"
     gene            complement(333..887)
                     /locus_tag="PLY93_15090"
     CDS             complement(333..887)
                     /locus_tag="PLY93_15090"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRP70848.1"
                     /translation="MEAALQPFVSYIAARRSGISGVEVADGAAEDNDEISIILFEIGQ
                     TQFALPTMRVIEVVRVEEIVAIPRAPSFIEGVIEVRDAVLPVIDLRKKLGARAERHAL
                     STIIVAEVQRLKTGFIVDSAKKVITLAGDEMQNLGTAVTGQEARYIYRTINRDKTPIV
                     ILNLDTLMNSHELESLAELEGGKA"
     gene            complement(908..>1342)
                     /locus_tag="PLY93_15095"
     CDS             complement(908..>1342)
                     /locus_tag="PLY93_15095"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRP70849.1"
                     /translation="HLFSMKEKQRLVLGEFLNSEVMNFNEPAEGVVRYNMTYALDLLG
                     SLKGRVLLNLDSSLAHAVAAILFASETGVSDEDMHVAMQELLNIFGGHLVTAFNELGH
                     DLDIATPERSDEDFMTAQATRRILFRFSCLGRIIQFVVLLKN"
BASE COUNT          327 a          350 c          322 g          343 t
ORIGIN      
        1 ctgtgactct gtggttttgt tttcaatgag acccaaccca attttggcga tccccgatgc
       61 gcctttcaaa attttcgcaa gcgtttgctt caccgacgcc ttgtcgcccg cgcacgttaa
      121 acggattaaa tacttgaatt gtgtgtcgat ggtctcttcg gaaagattgt cgcctggaaa
      181 aatatcgggc gtgatcgact ctaacatgaa ttggtcttct gccgacgcca agcggcggta
      241 aatttcacgg atcgcgatgc tggggaattt ggtttcggcg ttgatttgaa tcgccacgcc
      301 ccagaatgtt ggttgagtgt gaacggcaat cgtcatgctt tacccccttc aagctctgcg
      361 agcgactcga gttcgtgcga gttcatgagc gtgtctaaat tcaaaatgac aatcggcgtt
      421 ttgtcgcggt tgatggtgcg gtaaatgtag cgcgcctctt gtccggtgac ggcggtacct
      481 aaattttgca tttcgtcgcc ggcgagggta atcacttttt ttgcactatc gacgataaaa
      541 ccggttttga gacgttggac ttcggcgacg ataatcgtcg agagagcatg ccgctctgcc
      601 cttgcaccga gttttttacg caagtcgatc actggcaaca ccgcgtcgcg cacttcaatg
      661 acaccctcaa taaaagaagg cgcgcgcggg atggcgacaa tctcttcgac gcgcaccact
      721 tcaatcacgc gcattgtcgg cagagcgaat tgagtttgac cgatttcaaa aagaataatc
      781 gagatctcat cgttatcttc ggctgccccg tcggcaacct cgacgccgct tatacccgaa
      841 cgccgcgcag caatgtaaga gacaaacggt tgtaaagcgg cttccatgat aaatccctct
      901 ttgttcttta attttttaac aacacaacaa attgaataat gcgccccaaa cacgaaaaac
      961 gaaacaggat acggcgcgtc gcttgggcag tcataaaatc ttcatcgctt ctttcgggag
     1021 tcgcaatgtc taagtcgtgc cccaactcat tgaacgcggt gacaaggtgc ccgccaaaaa
     1081 tattaaggag ctcttgcatc gcgacgtgca tgtcttcgtc ggagacgccc gtttcactcg
     1141 cgaataaaat cgccgccacc gcgtgcgcaa gactcgaatc taagtttaat aaaacgcgcc
     1201 ccttgagcga acccaacaag tcgagtgcgt atgtcatgtt gtagcgcacc acaccttcgg
     1261 caggctcgtt gaagttcatc acctctgaat tcaaaaattc accgagcacc aggcgctgtt
     1321 tttctttcat cgagaataaa tg
//
LOCUS       DAOMPD010000649         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_35818_length_1340_cov_2.710506, whole genome shotgun sequence.
ACCESSION   DAOMPD010000649 DAOMPD010000000
VERSION     DAOMPD010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35818_length_1340_cov_2.710506"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..979)
                     /locus_tag="PLY93_15100"
     CDS             complement(146..979)
                     /locus_tag="PLY93_15100"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRP70850.1"
                     /translation="MTLLLYEKVRARSEGFGASLPSKKQAGEFIAELFTFLFAHSYHA
                     RQLTRVEWEKHVFILQENLSRLLTAFVVKEKAAEISKIFFYEELPRLYEILWQDAEAG
                     YTGDPAAESVQEVILAYPGFYAVTVYRIARYIHSFKVPTLPRLFSEHAHEVTGIDIHP
                     GAQIGRSFFMDHGTGIVIGGTSIIADNVKIYQGVTLGALSVEKNLASTKRHPTIEEGV
                     VIYAGATILGGDTVIGRHATIGGNTWLVESVPPYSVVYHKSEVRVRPSKELDGVIDFI
                     V"
     gene            complement(976..1161)
                     /locus_tag="PLY93_15105"
     CDS             complement(976..1161)
                     /locus_tag="PLY93_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70851.1"
                     /translation="MARRLANKGRRNLPKTTEIGRKAFSSTQARITEYKDRIRDRNYL
                     DFAIDRIAIELTHFLTK"
BASE COUNT          363 a          312 c          289 g          376 t
ORIGIN      
        1 gacaaaggag atgcgcttca ccgccgaaaa gtcctctgtc gtgtaaaaac ggccccccat
       61 gtggacaaaa gcttgcggtt tgcaagtgat gtattttcat cgggcaattt tagctaagta
      121 ttttgcgcgg caagcaataa gaaggttata caatgaaatc aataacaccg tctaactctt
      181 tcgatggtct cacgcgcact tcgcttttat ggtacacgac agaatatggt ggcacacttt
      241 cgacgagcca tgtgttacct ccaatcgttg catgccgccc aatcacggtg tcaccgccga
      301 ggattgttgc gccagcataa ataaccaccc cttcttcgat ggtgggatgt cttttggtac
      361 tcgctaaatt tttctctacg gaaagtgctc ccaacgtcac accttgataa atttttacat
      421 tatcggcgat aatgctggtg ccaccaatca caattcccgt accgtggtcc ataaaaaatg
      481 agcgaccaat ttgcgcgccc ggatggatat cgataccggt cacttcgtgc gcatgttcgc
      541 tgaaaaggcg cggcagtgtc ggtactttga aagaatgaat atagcgcgca atgcggtaga
      601 ctgtcaccgc ataaaaacct ggataggcaa gaatcacttc ttgtacgctt tcagcagccg
      661 gatcgcccgt gtaccctgct tcggcgtctt gccaaagtat ttcatagagg cgaggcagct
      721 cttcataaaa gaaaatttta cttatttcgg cggctttttc tttgacgaca aacgcggtca
      781 aaagacgaga taagttttct tgcagtataa atacgtgttt ttcccactcg acccgagtta
      841 attgccgcgc gtgataacta tgcgcaaaaa ggaatgtaaa taattcggca ataaactccc
      901 ctgcttgctt tttactcggt aacgatgcgc caaacccttc gctccgtgcg cgaacttttt
      961 catagagaag taatgtcact tagttaaaaa atgcgtgagc tcaatcgcga tacgatcaat
     1021 ggcgaagtcg agataatttc tatcacgaat gcgatcctta tattctgtaa tccgcgcctg
     1081 tgtcgacgag aatgctttac ggccaatttc ggtcgttttg ggtaaattac gcctaccctt
     1141 attggcaagt ctacgagcca tagttacttc cttgtaacgc ggggtaaaac cccaaaatta
     1201 ggccatcctt ggcctaagat atacttcgac tcttatgcaa gtttgctata gtttttttta
     1261 ttcctaaaaa gtataatttc ttactctcgg cggggaggtc ggacaaattt ttagaaaaat
     1321 tagaaaaatt ataatgtgac
//
LOCUS       DAOMPD010000650         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36136_length_1330_cov_3.210196, whole genome shotgun sequence.
ACCESSION   DAOMPD010000650 DAOMPD010000000
VERSION     DAOMPD010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36136_length_1330_cov_3.210196"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLY93_15110"
     CDS             complement(<1..431)
                     /locus_tag="PLY93_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRP70852.1"
                     /translation="MSTQMSERLENIYQDIIKRNSGEIEFHQAVREVLESLGPVLVKY
                     PELAEQKIIERIAEPERQIIFRVPWQDDKGEIHINRGFRVEFNSSLGPYKGGLRFHPS
                     VYLGIVKFLGFEQIFKNALTGLPIGGAKGGSDFDPKGKSDN"
     gene            823..>1330
                     /locus_tag="PLY93_15115"
     CDS             823..>1330
                     /locus_tag="PLY93_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70853.1"
                     /translation="MRLWKGINKEGFLRYLPLVVYVTLTAFFLAVVYRVFLVYELKEI
                     YTDMHLAPWALVRQTALEDFLLAFLIALPAVLLLFPESRARKRFAAALTVIFIFFYGT
                     LLSASLLFVTIFEAPFQSTFFGQSLGTFQNAMFTSTLSELSLIPFRKAAPVFILMFAT
                     IVGFLFSRF"
BASE COUNT          327 a          290 c          261 g          452 t
ORIGIN      
        1 tcattgtcgc ttttaccttt gggatcaaaa tccgaaccgc ccttggcgcc gccaatgggt
       61 aggcctgtca gggcattttt aaaaatttgt tcaaacccta aaaattttac aatccccaga
      121 tacacagaag gatggaaacg taatccgcct ttatatggcc ccagcgatga attgaattca
      181 acgcgaaagc cacggttgat gtgaatttct cctttgtcgt cttgccaagg gacgcgaaaa
      241 ataatctggc gctcgggttc ggcaatacgc tcgataattt tttgctcggc taattcggga
      301 tatttaacca acactgggcc gagagattct aaaacctcac gcacagcttg gtggaattcg
      361 atttcaccag aatttctttt aataatatcc tggtaaatat tttctaatct ttcactcatt
      421 tgggtactca ttttctactc ctataagatt cccaatacaa aatctttgtt atttttaatt
      481 ttatcagttc aaagatttta tccctgtcgt ctttctgtcg cttgacggcc agatttccat
      541 cttcttctat tggaccctaa atctggtaaa atcgattttt tgtcgggagt atctccatga
      601 cgcgtcaatt aaattttaaa acgatgtctt gaaagcctgt gcgccaacga gcatagacat
      661 cctttttttt acaaaatttc gcgcttttga gcgaatcgcg tataaaaatt tgtaaaaaaa
      721 ttgcctcttt gtacacgcga gttggcgcac aggctcttaa taaatatcac cgccaaaatt
      781 cgatttagct tttcagcaag tgcgctttct ctcgtctaac aaatgcgatt atggaaaggc
      841 ataaataaag aaggcttttt aaggtatcta ccgttggtcg tgtacgtgac attaacggca
      901 ttttttttag cggttgtgta tcgtgtattt ttggtttatg aactcaaaga aatctatacc
      961 gatatgcatt tggctccttg ggcgttggtg cggcaaactg cactggaaga ttttctactg
     1021 gcgtttctca ttgcgttgcc tgcggtactt ttacttttcc cagaaagccg agcacgaaaa
     1081 cggttcgctg cggcgctgac ggtcattttt atattctttt atggcacatt gctttcagca
     1141 tcgctcctct ttgtcaccat ttttgaagcg ccgttccaaa gcacattctt tggtcaatcg
     1201 cttggtactt ttcaaaacgc aatgtttacc tcgacgctct cggagttgtc gttgattcct
     1261 ttccgtaaag cggcgcccgt ttttatttta atgttcgcga cgattgtggg ttttttgttt
     1321 tcacgatttc
//
LOCUS       DAOMPD010000651         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36329_length_1325_cov_2.650394, whole genome shotgun sequence.
ACCESSION   DAOMPD010000651 DAOMPD010000000
VERSION     DAOMPD010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36329_length_1325_cov_2.650394"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLY93_15120"
     CDS             complement(<1..838)
                     /locus_tag="PLY93_15120"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRP70854.1"
                     /translation="MEEENAFKLEKDIEFLHSRFEKILIESEGESFYHSVKETLKLSR
                     AYREKHSEQEFQELIRLIEKTPLVDAEKLTRALTVYLTLVNIAEEHHKVRLTRQENEG
                     AMAKTFAALLNADIPQDKLYDTVCNLRIELVLTAHPTEIMRRSLLQKYTNISLALQIR
                     DYADVPLFEREQNDATISREIYGIWQTDAIRKKQPTPTDEAYGGLLIFEQTLWNEMPR
                     YLRELSLALKEATGRTLPLDAAPMRFASWMGGDRDGNPNVTEAVMRRAMARQSKAALD
                     YYL"
     gene            complement(853..>1325)
                     /locus_tag="PLY93_15125"
     CDS             complement(853..>1325)
                     /locus_tag="PLY93_15125"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HRP70855.1"
                     /translation="PGEVQAFDGYNIHVEKYEEKMMPEGARVPLYGTRLVTVAVYKNN
                     KLVGRDTTEIRTYPMFSFRDAAFSDSQNTSEPAIVSTLFRDIYVQLGGDEKGRLVMQV
                     WINPLVRWVWAGFAFYVIMGLLLLLPIGEKKVFRLGKLSFSITPQPKAVTARNA"
BASE COUNT          291 a          328 c          361 g          345 t
ORIGIN      
        1 ccagatagta gtccagcgcc gctttcgact ggcgcgccat cgcccgccgc atcaccgcct
       61 cggtgacgtt ggggttgccg tcgcggtcgc cgcccatcca tgaggcaaag cgcatgggcg
      121 cagcgtctaa agggagggtg cggccggtgg cttctttaag cgcaagcgac aattcgcgta
      181 agtaacgcgg catttcattc cagagagttt gctcaaaaat caataatcca ccgtacgctt
      241 cgtcggtggg ggtgggttgt tttttgcgga tggcgtcggt ttgccatatc ccgtaaatct
      301 cgcggctaat cgttgcgtcg ttttgttcac gttcaaataa aggaacatcc gcatagtcgc
      361 ggatttgtag cgcaagagaa atgttggtgt atttttgtaa caaactccgc cgcataattt
      421 cggtgggatg tgcggtgagc acaagctcga tgcgcaagtt acacaccgtg tcgtaaagct
      481 tgtcttgtgg aatgtcggcg ttcaaaagcg cggcaaaagt tttagccatc gcgccttcat
      541 tttcttgccg agtcaatcgc actttgtggt gctcctcggc gatattcacg agcgtcaaat
      601 acacggtgag cgcgcgggtg agtttctctg cgtcgactaa aggagttttc tcaataagcc
      661 ggatcagctc ttgaaattct tgctcgctgt gcttttcgcg gtacgcgcgc gagagcttta
      721 gcgtctcttt aacgctatga taaaacgact cgccttcgct ctcgataagg attttctcga
      781 agcgggaatg caaaaactca atgtcttttt cgagtttaaa tgcattctct tcttccattg
      841 tttggcgtaa tgctacgcgt tgcgagcggt gacagctttg ggttgtggtg ttatagagaa
      901 gctcaactta cccaaacgaa aaactttttt ctctccgatg ggtaaaagaa gaagaagccc
      961 cataattacg tagaacgcaa agcccgccca aacccaacgc acgagtgggt taatccacac
     1021 ttgcatcacg aggcggcctt tctcgtcgcc gcccaactgc acatagatat cgcgaaagag
     1081 cgtcgatacg atggcgggct ccgacgtgtt ttgtgaatcg gagaatgccg cgtcgcggaa
     1141 gctaaacatg gggtaggtac ggatttcagt ggtgtcacgg cccacgagct tgttgttttt
     1201 gtagactgcg acggtgacaa gacgtgtgcc atagagcggc acgcgcgcgc cttcgggcat
     1261 cattttttct tcgtattttt caacgtggat gttatagccg tcgaaagcct gcacttcgcc
     1321 aggag
//
LOCUS       DAOMPD010000652         1322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36449_length_1322_cov_2.071823, whole genome shotgun sequence.
ACCESSION   DAOMPD010000652 DAOMPD010000000
VERSION     DAOMPD010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36449_length_1322_cov_2.071823"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..736
                     /locus_tag="PLY93_15130"
     CDS             146..736
                     /locus_tag="PLY93_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRP70856.1"
                     /translation="MDKIGEKTAWQIYPWRDSKERILIGATVAFSRKGFYGTSVREIS
                     HEAGLEQPSIYHHFGSKENLYWTCLRATHLFMIRTLRHRLTKQVSLEEEIRSMFHAIS
                     WFHQKYPEFFKLMFALIYSSPPGIGDRYTTLYGGDIFDFVERAFSRHPVETGRLEKYS
                     LTVHTFYSYILAYSGRLPDNLRVSYFQALKKLFKAF"
     gene            complement(867..>1322)
                     /locus_tag="PLY93_15135"
     CDS             complement(867..>1322)
                     /locus_tag="PLY93_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HRP70857.1"
                     /translation="HDTVEKASTEIADQTHLVRIPLDRNCPQCGNQLMQKLGKNGYFI
                     GCSNFQGGCRFSESIPLGVCPLCNGNVVKKKGKKGRGFFGCANYSTTGCEFTMLDTPA
                     KRTCPQCGSIMGQKVKKSGITLTCQNPECKHVIEETTDDSGTEPTAQTA"
BASE COUNT          330 a          292 c          318 g          382 t
ORIGIN      
        1 agagtttgtg cgtcaattta ctgaggtaat tttttaaatt tttgtacacg ctgcgcgagt
       61 tggcgcacac gccctatagc atcaaacttt agagttggcg catagactcg aaattttaga
      121 tacgcgtaga ttctcctcaa ctttcatgga caaaataggc gaaaaaacgg cgtggcaaat
      181 ttacccgtgg cgcgactcta aagaacgtat tttaatcggg gcgacggtag cattttcacg
      241 caagggattt tacggcacat cggtgcgcga gataagccac gaggcggggt tggagcaacc
      301 gagtatctac catcattttg gaagcaaaga aaatttatat tggacatgcc tgcgggcgac
      361 gcatcttttt atgatacgca cgctgcgcca ccgtctcacg aagcaagttt cgttagaaga
      421 ggaaatccgc tcgatgttcc acgccatcag ttggttccat caaaaatacc ccgaattttt
      481 taagttgatg tttgcgctta tttattcgtc gccgcccgga attggcgacc gttacacaac
      541 gctttacggt ggcgatattt ttgattttgt cgaacgcgcg tttagtaggc atccggttga
      601 aacggggcgg ttagaaaaat attcgctcac ggtgcatacg ttttatagct acatccttgc
      661 gtattcgggt aggctaccgg ataatttacg cgtgtcttat tttcaggccc tcaaaaaatt
      721 atttaaagca ttctaaaaaa atctaattgc tgagcgggtg gatttactaa aagatattct
      781 tgtaactctg aaatcttctt acattctgaa cgctttgtac tctgctatcc gcgcatacta
      841 caatttgtgt agaaaatcaa gctgtgtcaa gcggtttgcg ctgtgggctc tgtacccgaa
      901 tcatccgtgg tttcttcgat gacatgtttg cattcgggat tttggcaggt gagggtgatg
      961 ccgctctttt taactttttg tcccatgatc gaaccacact gcggacaagt gcgcttggcg
     1021 ggagtgtcga gcatggtgaa ctcgcaaccg gtggtggagt aattcgcgca gccaaaaaat
     1081 ccacgccctt ttttgccttt ctttttgaca acgttgccgt tgcacaaagg acacacgccc
     1141 aaaggaatgc tctctgaaaa acgacagccg ccttgaaagt tgctacaacc gataaagtag
     1201 ccgtttttac ccagcttttg catgagttgg ttgccgcatt gcggacaatt acggtcgagc
     1261 ggtatgcgaa ccaagtgtgt ttgatcggca atttcagtgg aagctttttc caccgtatcg
     1321 tg
//
LOCUS       DAOMPD010000653         1318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36561_length_1318_cov_2.489311, whole genome shotgun sequence.
ACCESSION   DAOMPD010000653 DAOMPD010000000
VERSION     DAOMPD010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36561_length_1318_cov_2.489311"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>815)
                     /locus_tag="PLY93_15140"
     CDS             complement(85..>815)
                     /locus_tag="PLY93_15140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRP70858.1"
                     /translation="QILTGTADYHLYFLVILAQCYLLFPFLLKFRHSYGKTYLWSTWT
                     IFAAIVFLLYKGTSEWVLPRLGLTHPAWHASFVVYWLPYFMLGILHGTMPMEAKPYRR
                     SLFVLAFLLCAAALALVFVEYDSYSRLGTPVDYYNHFSRPTVVLYTLAIIGLLFALPR
                     SVETKGGHGERLAALTFAVYLIHPQVLRIVTPSMLFLPSLVAIATFVLVYIFTALIKK
                     ASNANNPALLRKTAGLLQRCLGLR"
     assembly_gap    816..825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<826..1211)
                     /locus_tag="PLY93_15145"
     CDS             complement(<826..1211)
                     /locus_tag="PLY93_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRP70859.1"
                     /translation="MLRTTTDWAKVIAALAVVGIHSTSSAETNFALQHNYASLDFLGV
                     LVNQWARFSVPLFIYLSAYGLSRSTKIGESGFGGNYAAFVKKRLPTILVPYFFFSGIA
                     IAMEFHSWMSATGQSSFSPQLWKNIAS"
BASE COUNT          386 a          304 c          317 g          301 t
ORIGIN      
        1 gtgggttgca tagtaatgtc ggcctcgtat gcaacccaca gagataactg acaacatcgc
       61 catccaaggg ctggcattca aacctcatct taaccctaaa caacgctgca atagccccgc
      121 tgtttttctc agtaaagccg gattatttgc gttacttgct ttttttatga gcgcggtgaa
      181 gatatacacc aacacgaatg tagcaattgc tacaagactg ggaagaaaaa gcatcgaggg
      241 ggtaactatc cgcagcactt gtgggtggat tagatacacg gcaaaagtaa gcgcggcgag
      301 cctttcccca tgcccgcctt tggtctccac cgaccgcggg agcgcaaaaa gtagaccgat
      361 gatcgccagg gtgtagagca caaccgtggg ccgggagaag tggttatagt aatcgacggg
      421 agtgccgagg cgggaataac tatcgtactc aacgaaaaca agcgccagcg cggcggcgca
      481 aagaagaaac gcgagaacaa aaagactgcg ccgatagggc ttggcctcca tcggcatcgt
      541 accgtgcaaa atgcccagca tgaaatatgg caaccaatac accacgaacg acgcatgcca
      601 cgcagggtga gtcagaccta aacggggcaa tacccattcg gatgttcctt tataaagaag
      661 gaaaactatt gcagcgaaga tagtccacgt actccacaga tatgtttttc catagctatg
      721 ccggaatttt aagaggaacg ggaagagtag atagcattgc gccaggatta ctaaaaagta
      781 cagatggtag tccgctgtac cggttaggat ttgcgnnnnn nnnnngacgc tatgtttttc
      841 catagctgtg gggaaaaact gctttgtcct gtagcgctca tccagctatg gaattccata
      901 gctatggcaa ttcccgaaaa gaaaaaatat ggtaccagaa tcgtcggtaa acgctttttc
      961 acaaacgcgg catagttacc cccaaaacca ctttccccga ttttagtaga gcggctcagt
     1021 ccatacgctg aaagatagat aaacaaaggc acactaaacc gcgcccattg gttgacgaga
     1081 acacccaaga agtcgagcga agcatagtta tgctgcaacg caaagtttgt ctcggcgctg
     1141 ctcgttgaat ggatcccgac aacggcaagc gctgcaatga ctttagccca gtcggtagtt
     1201 gtgcgcaaca ttatgtcgca ttatttttaa agcttttttt gtcaattttt taataaaata
     1261 tttgtctaaa ccctcaagac atatgttaca ctttttataa agtgtttaag gtaggtgg
//
LOCUS       DAOMPD010000654         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36604_length_1317_cov_2.315372, whole genome shotgun sequence.
ACCESSION   DAOMPD010000654 DAOMPD010000000
VERSION     DAOMPD010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36604_length_1317_cov_2.315372"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLY93_15150"
     CDS             <1..395
                     /locus_tag="PLY93_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70860.1"
                     /translation="LQILRIGMFYSNSVRGIALLAQRRFMREKNFHSEWHDEWKASGK
                     KFSDFFYDKNYRSEEETYREHANGVPFRPTEPGDIADAFCIVFQGEKSPIINVLGAWR
                     WFENTMPHLPEAITSRMHLIDPYIYELV"
     gene            481..>1035
                     /locus_tag="PLY93_15155"
     CDS             481..>1035
                     /locus_tag="PLY93_15155"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HRP70861.1"
                     /translation="MNLVTDTTSLQAGAVPLVDVQSSRDNRHIAIDKVGIKGIRYPIK
                     ISDKTGEVQNTVAELTMAVFLPQQFKGTHMSRFVEILEARHKSLDVQTFKELMPEMLH
                     RLESQAGEIQMRFTYFIMKKAPISGVESYMDYDVTFVGKSEKGKHGLQIKVVVPVTSL
                     CPCSKEISKYGAHNQRSHITITAKL"
     assembly_gap    1036..1064
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1065..>1317
                     /locus_tag="PLY93_15160"
     CDS             <1065..>1317
                     /locus_tag="PLY93_15160"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HRP70862.1"
                     /translation="TLWIEDLIRIAEESASCELYGLLKRPDEKFVTEHAYDNPKFVED
                     IVRDVAHKLNEDNRISHYIAEAENFESIHNHSAYALIEKD"
BASE COUNT          372 a          292 c          300 g          324 t
ORIGIN      
        1 gtttgcaaat tttacgtatc ggtatgttct attccaattc ggtgcggggg attgccctct
       61 tagcgcagcg gcgtttcatg cgcgaaaaaa atttccactc cgagtggcac gacgagtgga
      121 aagcttcggg gaaaaaattc agcgattttt tttacgacaa aaattatcgt agcgaagaag
      181 agacgtaccg cgagcacgca aacggcgtgc cattccgccc caccgaaccg ggcgacatcg
      241 ccgatgcgtt ttgtattgta ttccaaggag agaagtctcc gattatcaac gtgctcggtg
      301 cgtggcgttg gtttgaaaac acgatgccgc atttgcccga agcgattact tcacggatgc
      361 accttattga tccttatatt tatgaacttg tatagttcat agtttttgga caccatcaaa
      421 actccttaaa tattcgcttt aattattgca gttttagact gattttagta taataaaagt
      481 atgaatctgg ttactgatac gacctcgtta caagcggggg cggtgccgct ggtcgatgtg
      541 caatcgagcc gcgacaaccg gcacatcgct atcgataaag ttggtatcaa aggcattcgt
      601 tatcccatca aaattagcga taaaacggga gaagtccaaa ataccgtcgc cgaattaacg
      661 atggcggtgt ttttgccgca gcaatttaaa ggcacacata tgtcgcgttt tgtggaaatt
      721 ctcgaagcgc ggcacaagtc tttggatgtg cagacgttca aagaactcat gcctgagatg
      781 ttgcaccggc tcgaatctca agcgggtgaa atccaaatgc gtttcaccta cttcattatg
      841 aaaaaagcgc ccatttcagg cgttgaatcg tatatggatt acgacgtgac ctttgtcggc
      901 aaaagcgaaa aaggaaaaca cggtttacaa attaaagttg tggtaccggt gacgagtctt
      961 tgcccgtgtt caaaagaaat ttcaaagtac ggagcgcata accaacgcag ccacatcacc
     1021 atcaccgcaa aacttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgctg tggattgaag
     1081 atttaatccg catcgccgag gaaagcgcgt cgtgcgaact ctacgggctt ttgaaacgcc
     1141 ccgatgaaaa atttgtcacc gagcacgcat acgacaatcc caaatttgtc gaagacattg
     1201 tacgcgacgt ggcacacaaa ctcaacgaag ataatcgcat tagtcactac attgccgaag
     1261 cggaaaattt tgagtcaatc cacaaccact cggcgtacgc gttgattgaa aaagata
//
LOCUS       DAOMPD010000655         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36606_length_1317_cov_2.229002, whole genome shotgun sequence.
ACCESSION   DAOMPD010000655 DAOMPD010000000
VERSION     DAOMPD010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36606_length_1317_cov_2.229002"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLY93_15165"
     CDS             complement(<1..423)
                     /locus_tag="PLY93_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70863.1"
                     /translation="MRFAVLFLFLTATLTYAKDEDVAFSRFSLSTYFSSRSMQFAPLD
                     DGKEIRPATLETYEVSSGTYSSDIKYRVAGERDAHSLAELGLRARAYSGNVEKATMMT
                     NFEFDILFDSKFEYFQSRLMGDMGIMFNPLPSLLIGPAV"
     gene            557..>1317
                     /locus_tag="PLY93_15170"
     CDS             557..>1317
                     /locus_tag="PLY93_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70864.1"
                     /translation="MLNKTVFRFLSLLLVTTPFTYLAADIQPLSDATLCNADSSPSGG
                     GCYLNDMNAVNQPADTQTQSLGVPNRLIENDMNMFVNPGQIMNYGTAYLEGRSSANLV
                     WGGATVPLPANQKLAVFVRRPMNANSLLGSVVGLLTKYSPALNVSNPLNGVTGVQMGI
                     LDTTAKGFGNLDAMYGLSMGDMNFGFRLSYANMKNNYDKTQTTGDISKYKISAHNIGV
                     GLGLQWKNLGPGYLDLSLSSDIAFFNASSNYTVTGV"
BASE COUNT          359 a          288 c          321 g          349 t
ORIGIN      
        1 gacggcgggg cctataagta aagaaggcaa tgggttaaac atgataccca tgtcacccat
       61 gagtcggctt tggaagtatt caaactttga gtcgaataaa atgtcgaatt caaaattggt
      121 catcatcgtc gctttctcga cgttgcccga atacgcacgc gcacgcaaac ccagttcggc
      181 gagcgaatgc gcatcgcgtt cgcccgcgac tcggtactta atatcagatg agtacgtacc
      241 agaagatact tcgtatgttt ctaaagtagc aggacggatt tctttgccgt cgtccaaagg
      301 tgcaaactgc atgctgcggc ttgaaaagta ggtcgataac gaaaatcttg aaaacgccac
      361 gtcttcgtct tttgcatacg tgagcgtggc ggttaagaat aaaaacaaaa cggcaaaacg
      421 catggcatgg atgctctttt aagaaaagat atggtgtaac gaagatatag gattcagcga
      481 gttggcgcac aggctcctta taatcaagag gaataaaatt atttgacata cgcagtcaga
      541 aatggtgcgt atcccgatgc tgaataaaac cgtatttcgt tttttatctt tacttttagt
      601 aactacgccg tttacctatc ttgcggcgga tattcaacca ctttccgatg caacgctctg
      661 taatgccgac tcctcgccaa gcggcggggg ttgttattta aacgacatga atgcagtcaa
      721 tcagcccgcc gatacgcaga cccagagttt aggtgtgccg aatcggctca tcgaaaacga
      781 tatgaatatg ttcgtcaacc cggggcaaat catgaattac ggcaccgcgt acctggaagg
      841 tcgaagctcc gctaacctcg tttggggggg ggcaaccgtg cctttgccag cgaaccaaaa
      901 attggcggtt tttgtacgcc gtcctatgaa tgcgaacagt cttttaggct ctgttgtggg
      961 tcttttgact aagtattctc ctgcgctcaa tgtgagtaat ccgttgaatg gtgtgaccgg
     1021 tgtacagatg gggatactcg atactacggc taaaggcttt ggtaatctcg acgcgatgta
     1081 cggtctcagc atgggcgata tgaatttcgg ttttcgccta agctatgcaa atatgaaaaa
     1141 taattatgac aagactcaaa ccaccggcga tatttcaaaa tacaaaatta gcgcgcacaa
     1201 catcggtgtc ggcttaggtt tgcagtggaa aaacttgggg ccgggctatc tcgacctttc
     1261 gttatcttcg gatattgcat tctttaatgc aagttctaat tatacggtca caggtgt
//
LOCUS       DAOMPD010000656         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36615_length_1317_cov_2.015055, whole genome shotgun sequence.
ACCESSION   DAOMPD010000656 DAOMPD010000000
VERSION     DAOMPD010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36615_length_1317_cov_2.015055"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLY93_15175"
     CDS             complement(<1..345)
                     /locus_tag="PLY93_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70865.1"
                     /translation="MKFIETLRLENGKIPLVELHQARVDLTLLAHYGSAVKITLLPAL
                     TSLHKPRMGLFKIRITYSSYLESIVIEPYTRRRVTAITLVPAEGLDYRYKYADRRALN
                     NLRQNVPEGVEPI"
     gene            complement(342..>1317)
                     /locus_tag="PLY93_15180"
     CDS             complement(342..>1317)
                     /locus_tag="PLY93_15180"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRP70866.1"
                     /translation="AMEKHSLQSCCDAQIPFFAVIDFEANTLVHALDVSSLKKENNSR
                     SGSIRLNGQNLHFRFANPQPLRQRFALQRVNPPDKAHTLLTIQQLQKHLHEGYSYLVN
                     YCTQTGVKLPLSPMELFLQSTAPFTIYYENEFVAFSPEAFVSVEGDTICATPMKGTGY
                     IKEELLASTKEQAEHATVVDLLRNDLARVAKEVRIENYRYITEIPQADGRTLYQTSTA
                     ICGTMPPDWRRSIPIWLPHLLPAGSVTGAPKRETVSLIRRYETEPRGFYTGIAALFDG
                     ENFYSCVLIRYIDLRTQPYQFRSGVGITIYSDAEAEYEEILQKVYIPL"
BASE COUNT          325 a          300 c          340 g          352 t
ORIGIN      
        1 aatcggctcc actccttcag ggacattttg ccgcaagtta ttgagcgcgc ggcggtcggc
       61 gtatttatag cggtaatcga gtccttcggc aggtacgaga gtaatcgccg tcactctacg
      121 gcgggtgtag ggttcgatga caatactttc aagatagctg gaataagtga tgcgtatttt
      181 gaaaagtccc atacgcggct tgtggagaga ggtgagtgcg ggcaaaagag taatttttac
      241 cgcgctgcca tagtgcgcca agagcgttaa atccacccgc gcttggtgta actcgacaag
      301 agggattttt ccattttcta aacgcaacgt ttcgataaat ttcatagggg aatatacact
      361 ttctgtaaaa tttcttcgta ctctgcttcg gcatcgctat agatagttat ccccacaccg
      421 ctacggaatt ggtatggctg cgtacgcaag tcgatatagc ggatgagcac acaactgtaa
      481 aagttttcac cgtcgaaaag cgcagcgatg ccggtataga aaccacgcgg ctcggtttcg
      541 tagcggcgga tgagcgacac cgtttctcgt ttcggcgccc ccgtcaccga ccctgcaggt
      601 aaaagatgtg gtaaccatat tgggatgctg cgccgccaat caggtggcat cgttccacaa
      661 attgcagtgc tggtttggta gagggtacgc ccgtcagctt gcggtatttc ggtaatatag
      721 cggtagtttt caatgcggac ttccttggcg acacgcgcta aatcgttgcg caataaatcc
      781 accaccgtag cgtgctctgc ctgctctttt gtgctggcga gcaactcttc tttgatgtag
      841 cccgtgcctt tcatgggagt ggcgcagatg gtatcccctt caacgctgac aaacgcctcg
      901 ggagaaaatg cgacaaactc gttttcgtaa taaatagtaa aaggggctgt cgattgtaaa
      961 aacaactcca tgggggaaag cggcaactta accccagttt gggtacaata attcacaagg
     1021 tacgaatagc cttcgtggag atgcttttgc aactgttgta tagttaataa cgtgtgtgcc
     1081 ttatccggtg gatttaccct ttggagtgcg aaacgctgcc gtagtggttg ggggttagca
     1141 aaccgaaaat gcaaattttg gccgtttagg cggatactcc cacttcttga attattttcc
     1201 ttctttaatg agctaacatc cagagcatgg acgagggtat ttgcctcaaa atcgatcacc
     1261 gcaaaaaaag gtatttgtgc atcgcaacag gattgtaagc tgtgtttttc catagct
//
LOCUS       DAOMPD010000657         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36710_length_1314_cov_2.480540, whole genome shotgun sequence.
ACCESSION   DAOMPD010000657 DAOMPD010000000
VERSION     DAOMPD010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36710_length_1314_cov_2.480540"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..477
                     /locus_tag="PLY93_15185"
     CDS             70..477
                     /locus_tag="PLY93_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRP70867.1"
                     /translation="MENQTPTKGAFWGGWVLSGLAIAFLTFDGVLKFFLHKMPPEAMA
                     DSAKLAYPLHIMPYLGATLLVCVLLYAIPHTAVLGAILLTGYLGGAVSAHVRILNPWP
                     SHILFPVYFGVFIWLGLYLRMPALRAFVPVRKG"
     gene            629..1195
                     /locus_tag="PLY93_15190"
     CDS             629..1195
                     /locus_tag="PLY93_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRP70868.1"
                     /translation="MKSTSVKKSVSSDTTLIDTLKSRFEKNMHRHKGMLWSKVLARIE
                     KNKYAFDALGLMEDTGGEVDVISSDKKSDAIIFCDCSPESPAGRRSLCYDREALNSRK
                     EHKPKNCAVDTARAMGIELLDEEWYRVLQTTGPFDTKTSSWLMTPSEVRKLGGALFGD
                     FRYGRVFIYHNGAESYYAARGFRGMLKI"
BASE COUNT          340 a          313 c          326 g          335 t
ORIGIN      
        1 gcgaccagca aaattggctg catagctgtc accgatgtct cggggttcta caaaattaag
       61 aggacaaaca tggaaaatca aacaccaaca aaaggcgcct tctggggcgg atgggtttta
      121 agcggccttg cgatcgcgtt tctcacgttc gacggcgtgc tgaagttttt cttacacaaa
      181 atgccgcccg aggcgatggc cgattcggcg aagctcgcct accctctcca tatcatgccg
      241 tatctcggcg cgactctgct cgtttgcgtt ttactctacg cgattccgca cacggcagtg
      301 ttgggagcga ttcttttgac tggatattta ggcggggcgg tttcggcgca tgtgcgtatt
      361 ctaaacccgt ggccgagtca tattctgttt cctgtttatt tcggcgtttt catttggttg
      421 gggttatacc tgcgtatgcc cgcgttgcgc gcattcgtac ccgtgaggaa aggatgaggc
      481 tcaacagtat ttacctaaac tttatagaca agaccttgtt tgccgacttc aaagagccga
      541 ttcgactcgg acaagcggcg ggcgtgtgaa ctaaagtcgc tttacaacgg tagcggaata
      601 ttcgcttgaa acaaatttca aacaatctat gaaatccacc tctgtaaaaa aatccgtatc
      661 tagcgacacc acgctcatcg acacgctgaa atcccgcttt gaaaaaaaca tgcaccgaca
      721 taagggtatg ctttggtcca aggtacttgc gcgcatcgaa aaaaataagt acgccttcga
      781 tgcacttggg ctcatggaag atacgggtgg cgaggtcgat gtcattagca gcgacaaaaa
      841 atcagacgcg attatctttt gcgattgctc acccgaatcg cctgcggggc ggcgtagcct
      901 ctgctacgac cgtgaggcac tgaattcgcg caaagagcat aagccgaaaa actgcgccgt
      961 cgacacagca agagcgatgg ggattgaact tttagacgaa gagtggtacc gtgtcttaca
     1021 gaccactggt cccttcgata ccaaaacgtc gagttggctg atgacaccct ccgaggtgcg
     1081 caagctcggc ggggcgcttt tcggcgactt tcgctatggt cgggtattca tctaccacaa
     1141 tggcgctgag tcgtactacg cggcgcgcgg ctttcgggga atgctgaaaa tataaaggat
     1201 aactatccaa actagtaatt gttttcaaaa aaatcacaat ttttctctaa aaattacact
     1261 ttttttcaga aaaatgagaa aatttgactg ttttctggta tttataatat agaa
//
LOCUS       DAOMPD010000658         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36712_length_1314_cov_2.363781, whole genome shotgun sequence.
ACCESSION   DAOMPD010000658 DAOMPD010000000
VERSION     DAOMPD010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36712_length_1314_cov_2.363781"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PLY93_15195"
     CDS             <1..1010
                     /locus_tag="PLY93_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRP70869.1"
                     /translation="PGEFTRRAYLNGKMDLDAAQAIQEIIDAKSATTLHAARRLQSGV
                     FRTALLQLRSALMNLLADLNAELDFIDEDISFATLDNKLQILRSAIDSATKLIHDAER
                     FNAYKDGVHIALVGKPNAGKSSLMNRLVGEERSIVSEIAGPTRDYIEGELTLAGFNVR
                     LFDTAGLNEESSDPIEKLGIERTHRLIERAHILILLIDGTDNAFPKNIPTVAAGAYTI
                     YAINKIDILPDIPREVDKIYISAKTGEGMPELTAAMEKHIYASTPQDAIPLHEWQQEI
                     LRRIVGHLTQAESALKANELAEVVSHLVQNAVTSIAELTGAIESEDILGRIFSRFCIG
                     K"
     gene            complement(1014..>1314)
                     /locus_tag="PLY93_15200"
     CDS             complement(1014..>1314)
                     /locus_tag="PLY93_15200"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRP70870.1"
                     /translation="MRFNAQGGMVGVFENQNFSERAILLSSGDRVVFLTDGILEHENE
                     TGEMYGTHRLSELFSKTTTIRAEQVCDALIEDMRLFRPSQSMLDDDATVIVLDID"
BASE COUNT          356 a          352 c          307 g          299 t
ORIGIN      
        1 aacccggtga attcacacgc cgcgcttatc tcaacggcaa aatggatttg gatgcagcgc
       61 aagccatcca ggaaattatc gacgccaaaa gcgccaccac ccttcacgcg gcgcggcgct
      121 tacaatcggg ggtgttccgt actgcgctcc tccagttacg ttcggcgctc atgaaccttt
      181 tggccgacct taacgcggaa ctcgatttta tcgacgaaga tatttctttc gcaactctcg
      241 acaataaact ccaaattcta cgcagcgcta tcgatagcgc cacaaagtta atccacgacg
      301 ccgagcggtt taatgcgtac aaagacggcg tgcatatcgc gcttgtggga aaacccaacg
      361 cgggaaaatc ctcgctcatg aaccgcctcg tcggcgaaga acgttcgatt gtgagcgaga
      421 ttgccggccc gacgcgcgac tacattgaag gcgagcttac ccttgcgggg tttaacgtgc
      481 gtctttttga taccgcggga ttaaacgaag aaagtagcga tcccattgaa aaactgggga
      541 ttgagcgtac acaccgcctc attgagcggg cgcatatcct cattctcctc atcgacggca
      601 ccgacaacgc ctttcccaaa aatattccca ccgttgcagc aggcgcgtat acgatttatg
      661 cgatcaacaa aatagatata ctaccagata taccgcgaga ggtggataaa atttatatct
      721 ccgctaaaac cggcgaggga atgcccgagc ttaccgcagc tatggaaaaa cacatctacg
      781 caagcactcc gcaagacgcg atccccttac acgaatggca gcaagaaatt ttacgccgca
      841 tcgtgggaca tctcacacag gcagaaagcg cgcttaaagc aaacgagctt gccgaggtgg
      901 tttcgcactt ggtgcaaaac gccgtgacga gtattgccga actcacaggc gctatcgaaa
      961 gcgaagatat tttagggcgt atctttagcc gattttgtat cggaaaataa atttcaatct
     1021 atgtctaaaa cgatgacggt cgcgtcgtcg tcgagcatgg actgcgatgg gcggaataga
     1081 cgcatgtctt caattaaagc atcgcagact tgctctgccc taatagttgt ggttttggag
     1141 aacaactcag agagtcgatg cgtcccatac atttctcctg tttcattttc atgctctaaa
     1201 ataccgtcgg tgagaaatac caccctgtcg cctgagctca gtagaattgc acgctctgaa
     1261 aaattttgat tttcaaagac ccccaccata ccgccttgtg cattgaaacg catc
//
LOCUS       DAOMPD010000659         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_36886_length_1309_cov_3.240032, whole genome shotgun sequence.
ACCESSION   DAOMPD010000659 DAOMPD010000000
VERSION     DAOMPD010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36886_length_1309_cov_3.240032"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLY93_15205"
     CDS             complement(<1..315)
                     /locus_tag="PLY93_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRP70871.1"
                     /translation="MEQTEEKKLNSLHTRLSKIRQTRVGDIMKKDVITLDASDLLATA
                     ARTLIENSIHGVIVMKDGKPWSVLSAFDLLHKSYIESFSDKMDYLRSSLDSLIEKPIL
                     YSL"
     gene            complement(369..1139)
                     /locus_tag="PLY93_15210"
     CDS             complement(369..1139)
                     /locus_tag="PLY93_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRP70872.1"
                     /translation="MGKYVIPDKATDEVARFLARSIKFLPEGFFDILAAYFRLEIEGE
                     ENLPNNGRGRMLIVPNHSNALGYDAFMIIMALKKITRRIPRTMGHTFWFSNGFLAAFS
                     RAMGTFPADLKEGLNALRDDNMVVIFPEGAEGNFKVSSTMYKMVDFNPGFVPLAIMQQ
                     APVVPAAIIGAEESTINLAKIDWFKDIIGASIPVPLNLIPFPAKWKIKFLPAIDFSKY
                     NKKDIKDARFVKEINQNIRYRIQNELNKEVSKRNIAFK"
BASE COUNT          335 a          271 c          330 g          373 t
ORIGIN      
        1 gagcgagtag agaatgggtt tttcgatgag gctatcgaga gacgagcgga gatagtccat
       61 tttgtctgag aaactttcaa tataactttt atgcaaaaga tcaaacgcgc ttaaaaccga
      121 ccacggcttg ccgtctttca tgacaatcac gccgtggatg ctgttttcga tgagagtgcg
      181 tgcggcggtg gccaggagat ctgaggcgtc gagggtgatc acgtcttttt tcatgatgtc
      241 accgacgcgg gtttgacgaa ttttagaaag acgggtgtgc agcgagttga gttttttttc
      301 ttcggtttgt tccataaatc cttcttacat atttatatag ttgtacgata tttaatccgt
      361 ccacagtgtt acttgaatgc gatattgcgt ttagagactt ctttgttgag ttcgttttgt
      421 atccggtagc ggatgttttg attaatttct ttgacgaaac gcgcgtcttt aatgtctttt
      481 ttattgtatt tgctgaaatc aatcgcgggc aaaaatttta ttttccattt tgcagggaaa
      541 ggaatgaggt tgagcggcac ggggatcgat gcgccgataa tgtctttgaa ccaatcgatt
      601 ttagcgaggt tgatggtgct ttcttcggcg ccgatgattg cggcgggtac aaccggcgct
      661 tgttgcatga tggcgagcgg tacaaagccg ggattaaaat cgaccatttt atacatagtc
      721 gacgagacct taaaattccc ttctgcgcct tcgggaaaaa taacgaccat gttgtcgtcg
      781 cgcagcgcgt tgagaccttc ttttaagtcg gcggggaaag tccccatggc acggctaaac
      841 gcggctaaaa atccattcga gaaccaaaaa gtatgtccca tcgtgcgggg tatacggcgt
      901 gtaatttttt taagcgccat aataatcata aacgcgtcgt aacccaacgc attcgagtgg
      961 ttggggacaa tgagcatgcg ccctctgccg ttgttgggta aattttcttc accttcgatt
     1021 tctaaacgaa aatatgccgc taaaatatca aagaaacctt cgggtaagaa ttttatcgag
     1081 cgcgccagaa agcgcgcgac ttcgtcggtt gctttatcgg gtatgacata ttttcccaca
     1141 gcccacaaat gccgatacga tgcaatgtgc gtcaagcaga aagagctttg cccgtatgca
     1201 acccacagag ataactgaca cttttggggt caagttgagt gcgttttaga aacactccac
     1261 cctaggttga gctacccagc aagaccaagg gggagcaatg cgaaaatta
//
LOCUS       DAOMPD010000660         1302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_37145_length_1302_cov_2.562149, whole genome shotgun sequence.
ACCESSION   DAOMPD010000660 DAOMPD010000000
VERSION     DAOMPD010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37145_length_1302_cov_2.562149"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..>1302
                     /locus_tag="PLY93_15215"
     CDS             252..>1302
                     /locus_tag="PLY93_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRP70873.1"
                     /translation="MSTQANEATAPEVRPVENRVFPPVARGMYDPQFEKDSCGVGFIA
                     NLKGGKTRTIIDKALAMLLNLEHRGAAGADPQTGDGAGILMQMPHDFMCIAAAEAKIQ
                     LPNEGDYGVAFVFMPQERPIRKQVENIFEKIVVDEDLEFLGWRKVPVDDNVPGYGAKL
                     TLPYIKQAFIGRGQFKGSADDFERRLYLVRRVIDQRIRVELKLNRAQYYVPSFSSRTI
                     IYKGMLLAAQVPAFFKDLRHKEMKSAMALIHMRFSTNTFPTWDLAHPFRLIAHNGEIN
                     TLRGNINWMKTREAVMESPHYGPDLKRMLPIVMEGQSDSATFDTVLELLVMSGRSLPH
                     AMMMMIPEAWSKNDLM"
BASE COUNT          353 a          303 c          326 g          320 t
ORIGIN      
        1 aaaacgcact caacttgacc ccaaaagtgt cagttatctc tgtgggttgc atattaaatt
       61 ttgctttatt cttgacgcgg caaccgctaa aaaatgtagc ggatcaatga gatgcactac
      121 ttgtaaaatt aggttttagc gagtggtttg aagagcgtcc catcgtttgt gcgcggtagt
      181 agcagaaaat cgcacacgaa agatgagttt gaaaaattaa gatttttttg ggatcttata
      241 ggagtagaaa aatgagcacc caagcaaatg aagcaacagc gccagaggtg cgtccagtgg
      301 aaaaccgcgt ttttccaccg gtcgcccgtg gtatgtacga cccgcagttt gaaaaagatt
      361 cgtgcggagt gggttttatc gctaacttga aaggcggtaa aactcggacg attatcgaca
      421 aagctttagc catgctcttg aacttagagc atcgcggcgc agcaggcgca gatccacaaa
      481 ccggcgacgg agcggggatt ctcatgcaaa tgccacatga cttcatgtgt attgccgccg
      541 cggaagcaaa aatccagtta cctaacgaag gcgattatgg cgtcgcgttt gttttcatgc
      601 cgcaagagcg gcccatccgt aaacaagtcg aaaacatttt tgaaaaaatt gttgtcgacg
      661 aagatttaga atttttaggc tggcgcaaag ttcccgtcga cgacaatgta cctggctatg
      721 gcgcaaaact aaccctcccg tatatcaagc aggcgttcat cggcagaggc caattcaaag
      781 gcagtgccga cgattttgag cgccgcttgt atttagtgcg gcgggttatc gatcaacgta
      841 tccgcgttga gcttaaactc aaccgtgcgc aatactacgt tccttctttc tcgagtcgca
      901 ccatcatcta caaagggatg ctcctcgcgg cacaagtgcc ggcgtttttc aaagatttgc
      961 gtcacaaaga aatgaaatcg gcgatggcgt taatccacat gcgcttttcg accaatactt
     1021 tcccgacgtg ggatctcgcg catccgtttc gtctcattgc acacaacggt gaaatcaaca
     1081 ctttgcgcgg taacatcaac tggatgaaaa cgcgagaagc agtgatggaa tcgccgcact
     1141 acggccccga cttaaagcgg atgctaccta ttgtgatgga aggacaatcc gactcggcga
     1201 cttttgatac cgtgcttgaa cttttggtga tgagcggtcg ctcgttgccg cacgcgatga
     1261 tgatgatgat ccccgaggcg tggtctaaaa acgatttaat ga
//
LOCUS       DAOMPD010000661         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_37713_length_1286_cov_2.625508, whole genome shotgun sequence.
ACCESSION   DAOMPD010000661 DAOMPD010000000
VERSION     DAOMPD010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37713_length_1286_cov_2.625508"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLY93_15220"
     CDS             complement(<1..416)
                     /locus_tag="PLY93_15220"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRP70874.1"
                     /translation="MRTKLTIFFVLLASALVVLAVTSSTSAAQVHLQLNEFQKELTHD
                     QNSSLLKRNITLYGKVKEGSIEREGVKARFRIIDGKNEMVVEHSGKTLLPDTFQDGAE
                     AGVEGYFDKNRSIFVSHKVMAKCASRYDAASTQLKT"
     gene            581..654
                     /locus_tag="PLY93_15225"
     tRNA            581..654
                     /locus_tag="PLY93_15225"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Asp,seq:gtc)
     gene            858..>1286
                     /locus_tag="PLY93_15230"
     CDS             858..>1286
                     /locus_tag="PLY93_15230"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRP70875.1"
                     /translation="MENKALRIPLLIVILGALIGLFFSAVLIYEHNGAATSVGSAVCD
                     AANASSCEKAATSTMGKILGFPLALYGFLFYGGMVALAVFLFISLHDTVMRLLYWGAV
                     AALAFDIFLFLYSITILGAVCRLCAVTYAATSIMVIGAFLL"
BASE COUNT          293 a          290 c          305 g          398 t
ORIGIN      
        1 tgtgttttta gttgagtgct cgcggcatcg tagcgagagg cgcatttggc catcacttta
       61 tgcgaaacaa aaatgctgcg gtttttatcg aaataccctt caacccctgc ctctgcccca
      121 tcttggaacg tgtcgggtaa cagagttttc cccgagtgct cgacaaccat ttcatttttg
      181 ccgtcgatga tacggaagcg cgctttcacg ccctcgcgct cgatgcttcc ttcttttact
      241 ttgccgtaaa gcgttatatt ccgtttcaag agcgatgagt tttgatcgtg tgttaattct
      301 ttttggaatt cattgagttg aaggtgcact tgcgccgccg atgtactcga tgtgaccgcc
      361 aacactacca atgcagacgc caagagaaca aaaaaaatgg tgagctttgt acgcatagtt
      421 gaaggtacaa aacagagccc cattcggcta agatatttgt atttttttcg agattctcat
      481 aaatttaatg atagtcgtag acagcatgtt tcataattaa attctgccag tctcataaaa
      541 gccgttgtgt gtgagtcact atacgcaacg agtcatgttt ggggttgtag ctcagttggt
      601 tagagcatcc gcctgtcacg cggaaggtca cgggttcgag tcccgccaac cccgaagttt
      661 ctctaatagt aaatttagtt aagggatgcc aactcggagc ctgtgtgaag cttgtgcgcc
      721 aactcgcgca gcgcgtacaa aaatttgcaa aaaaattgtt tcagcgagtt agcgcataga
      781 ttcctcgtag agtttgtgac cgtaatctct taaaatgctt gtccgcgtat gcggcgaaag
      841 agagtattca atatctcatg gaaaataaag cgttacgtat ccccctcctt attgttatcc
      901 ttggtgcttt gataggtctc tttttttcgg ctgtactcat ttatgagcat aatggcgcag
      961 caaccagcgt cggctcggcg gtttgcgatg cggccaacgc cagctcatgc gaaaaagcag
     1021 caacctcgac gatgggtaaa attttgggat tcccccttgc gctctacgga tttctttttt
     1081 acggcggcat ggtggcactc gcggtttttc tctttatttc actccacgat accgtgatgc
     1141 gtcttttata ctggggtgcg gtggcggctt tggcgttcga tatatttctt tttctttatt
     1201 caataactat cttaggcgcg gtgtgccggt tgtgcgctgt gacttatgca gcaacgtcga
     1261 ttatggtcat cggtgcattt ttatta
//
LOCUS       DAOMPD010000662         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_37792_length_1284_cov_2.150529, whole genome shotgun sequence.
ACCESSION   DAOMPD010000662 DAOMPD010000000
VERSION     DAOMPD010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37792_length_1284_cov_2.150529"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..678)
                     /locus_tag="PLY93_15235"
     CDS             complement(13..678)
                     /locus_tag="PLY93_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70876.1"
                     /translation="MIMTALSLHRNSLRRMVIVAVKRFAFVALLSLYAGALFAHVEEG
                     HLGAEFGYGIQDTSGSGPANIRMPTVALWYHLTDWVALNVRGGLSIVSYDSDYNNSEK
                     YSTTVYGASIEMPFYIAKLNAVRLYLAPNVGYTFTKDESFSVSGITGSSQWKTDSLYK
                     YVSFLTVVGLQVAILEQLHVVGRTTFGYVWQNVTYPTSNTYSHSSFFGFQSWSLGAIV
                     YFN"
     gene            complement(675..>1284)
                     /locus_tag="PLY93_15240"
     CDS             complement(675..>1284)
                     /locus_tag="PLY93_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70877.1"
                     /translation="KRSILIALLCLHTGVLSATVRGGRFGVEFGYGDTVDIRQAFPQT
                     VYVGTPGASLWYHFTDWIAITVRMGFRTNSANGSIQNLDGVLAHDQGPGLFWGTSVEV
                     PFYIAEFHSIHLYVAPGAGYTLQQSKYYLSSRQGEFFRYFATTGIQIPVIDQLHLVGR
                     ITFGYIHGSITQNTQDNSESVDLYPSYMGLQSWSLGAIIYLN"
BASE COUNT          383 a          308 c          284 g          309 t
ORIGIN      
        1 aaacggccat aatcaattaa aataaacgat cgcccccaac gaccagcttt gaaaaccgaa
       61 aaaagaacta tggctatatg tgttgctcgt tgggtacgtt acattctgcc agacataacc
      121 aaaagtggtt cttccgacaa catgcagttg ttcgagaatt gcaacctgca acccgactac
      181 ggtcaagaaa ctcacgtatt tatataaaga atcggtcttc cattgcgaag agccggttat
      241 gccgctcacg gaaaaacttt catccttagt gaaggtgtat cctacattcg gtgcaagata
      301 gaggcgaacg gcattgagct tcgcgatata aaacggcatt tcgatagaag ctccgtaaac
      361 cgttgttgaa tatttttctg agttgttgta atcgctatcg tacgagacta tagataagcc
      421 gccgcgcaca tttaaggcaa cccaatcggt caagtgatac cacaacgcaa cagtcggcat
      481 gcggatatta gcggggccgc tccccgatgt gtcttgaata ccgtagccaa actcggcgcc
      541 caaatgccct tcttcgacat gagcaaacag ggcgcctgcg tatagagaga gcagagcgac
      601 gaatgcaaat ctttttaccg ccacgataac catgcgtctt aacgaatttc tatgtaatga
      661 taaagcggtc ataatcaatt taagtaaata atcgccccca acgaccagct ttgtagcccc
      721 atatacgaag gataaagatc tacagactca gaattgtcct gcgtattctg cgttatacta
      781 ccgtggatat aaccaaatgt aattctaccg acgagatgca gttgatcgat aacgggaatc
      841 tgtatgccgg ttgttgcaaa atatctaaag aattctcctt ggcggctgga caaataatat
      901 ttgctttgtt gcaaggtata acccgccccg ggagcaacat acaaatggat gctatgaaat
      961 tcggcgatat aaaacggtac ttctaccgag gttccccaaa aaaggccggg cccttggtcg
     1021 tgcgctaata cgccgtcaag gttttgtata gagccgtttg cagaatttgt acgaaacccc
     1081 atacgtacgg ttatagcaat ccagtcggtg aaatggtacc acagagaagc accgggtgtg
     1141 ccgacataca ccgtctgtgg aaaagcctgt cgaatgtcga ccgtatcacc ataaccaaac
     1201 tcgacaccga accgcccacc tcttacggta gcagacagca caccggtgtg taggcatagc
     1261 aaagcgataa gtatgcttct tttt
//
LOCUS       DAOMPD010000663         1281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_37907_length_1281_cov_2.776509, whole genome shotgun sequence.
ACCESSION   DAOMPD010000663 DAOMPD010000000
VERSION     DAOMPD010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37907_length_1281_cov_2.776509"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLY93_15245"
     CDS             <1..569
                     /locus_tag="PLY93_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70878.1"
                     /translation="ERYADVDGMDDTLGAAPSVDDSSANDTESTLPLEEAPAHEELPA
                     LEETPALAAEETHAPAAIDEEDDGPIALSLDELEGIAADAHEVTPEAPMVPLAPMDTG
                     MNEPSLAENTDKPSTAFEEDLESATLVTEGEAVAKENEATPDSDELKSVMGYLDNLLG
                     ELPDDVIEKFAQSEYFKLYQKIMDKLGL"
     gene            573..>1281
                     /locus_tag="PLY93_15250"
     CDS             573..>1281
                     /locus_tag="PLY93_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRP70879.1"
                     /translation="MGLRERASDYSKTVGSQSGIDLGLELKPDTAEKRVAQEKKPLSL
                     SPSTHENNAPPTTPPLSSADFPENMGHKSRLDAVLNLVEIYKEFGQVRSAKDLWEIVA
                     YSLMAQLGTKYVAIFMEDEMRMELKHALGFTLASDYSFAANSTLAQALAREKKVLYTD
                     DFIASFSEREEKFITQLNARYAVPVFRYEELRGVILVNPPQGRNTFDSDDLFYLKLCG
                     ELLGAMEAQLRLIAGAEK"
BASE COUNT          351 a          348 c          312 g          270 t
ORIGIN      
        1 acgagcgtta cgccgatgtc gacggtatgg acgatacttt aggcgctgcg ccctctgtcg
       61 acgactcttc cgctaacgat acagaaagca ctctgcctct cgaagaagcg cctgcccatg
      121 aagagttacc cgcgctcgag gagacgccag cgcttgccgc cgaagagaca cacgcaccgg
      181 cggcaataga cgaagaagac gacggcccca ttgcgctttc actcgacgag ctcgaaggca
      241 tcgccgccga cgcgcacgaa gtcacccccg aagcgccaat ggtaccactc gcgccgatgg
      301 acaccggcat gaatgaaccc agtcttgccg aaaataccga taaaccctcg acagcttttg
      361 aagaggatct cgaatcggcg actctcgtta ccgagggtga agcagttgcc aaagaaaacg
      421 aggcaacgcc cgacagcgac gaacttaaat cggtgatggg ttacttagac aacctcttgg
      481 gcgaattacc cgacgacgtg attgaaaaat ttgcgcaatc ggaatacttt aagctttacc
      541 aaaagattat ggataaactt ggtctctaag ttatgggctt acgcgaacga gcgagcgatt
      601 attcaaaaac tgtcggctcg caaagcggca tcgatttagg gctcgaactt aaaccagaca
      661 ctgcggaaaa aagagtagcg caggaaaaaa aaccgctttc cctctctcca tcaacgcacg
      721 aaaacaacgc tccccctaca acgccaccgc tttcatcggc ggatttcccc gaaaacatgg
      781 ggcataaatc gcggctcgat gccgtattga acctcgttga aatttacaaa gaatttggcc
      841 aagtccgctc tgcaaaagat ttgtgggaaa tcgtcgctta ttcgctcatg gcgcagcttg
      901 gcacaaagta cgtcgccatt ttcatggaag acgaaatgcg tatggaactt aaacacgcct
      961 tgggatttac cctcgcgtcg gattattcat ttgcggccaa cagcactctt gcgcaagcgt
     1021 tggcacgcga aaaaaaagta ttgtataccg acgacttcat cgcctctttt tccgagcggg
     1081 aagaaaaatt cattacacag ctcaacgcac gctatgcggt gcccgttttt cgttacgaag
     1141 aattacgcgg cgttatcttg gtgaatcccc cccaaggtag gaacactttt gacagcgacg
     1201 atttattcta tttgaaactt tgtggcgagc tcttgggtgc aatggaagcg cagttacgcc
     1261 tcatcgccgg cgccgaaaaa a
//
LOCUS       DAOMPD010000664         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38065_length_1277_cov_2.587561, whole genome shotgun sequence.
ACCESSION   DAOMPD010000664 DAOMPD010000000
VERSION     DAOMPD010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38065_length_1277_cov_2.587561"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1186)
                     /locus_tag="PLY93_15255"
     CDS             complement(167..1186)
                     /locus_tag="PLY93_15255"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRP70880.1"
                     /translation="MALFDDCFNSEANTTLAPPDLDKFWKAQIARLKKIPIEMEAKKK
                     VSRKILTEQNLLIDFQSIDKYHCQAQLFVPRKMSGKPPLVLIFPDYGKEAVVYKGLMN
                     VGIAQMIVRMRGHEAPRPVPGDKQTGRKAEMQNDREKQKTEKQSAAYGYFGERLLEPT
                     EYYAAKIFLDAFRALEVARLRKEVDTSRIGIIGQGVGAAQALFATAFMERGDALYLEN
                     PAFLNLDDTQNISDADYAREINSNARGSKKVKQQIKENLRYFDAVYFADKIKTDCAFS
                     INLHNKEAVPHGAFALFHLLQCEKDMYLFTEGDPVALADEKRKTVLNAVKYFREKLLR
                     EKVED"
BASE COUNT          329 a          306 c          268 g          374 t
ORIGIN      
        1 agtggccgcc cgacactatg aactcatcga gaacatggcg ctttgtgtgg ccaaagacct
       61 caaaaaaatt tgcaacgccg cgcgcgttca agtgaaaatt gagaaaccac ttgcggcgga
      121 gaaaaacagc gcacaaaata tttttgtaga aattgaaatt tagagactaa tcctctactt
      181 tttctctcaa gagtttttcg cggaaatact tcactgcgtt caaaaccgtt ttgcgctttt
      241 catcggcaag tgcaacgggg tcgccttcgg tgaaaagata catatctttt tcacattgta
      301 gtaaatgaaa tagcgcaaac gccccgtgcg gcaccgcttc tttgttgtgc aaattgatcg
      361 aaaacgcaca atcggttttt attttgtcgg caaaatacac cgcatcaaaa tagcgtaaat
      421 tttctttaat ttgttgcttt acctttttac tgccacgggc attgctattg atctcccgtg
      481 cataatcggc gtcggatata ttttgagtgt cgtctaaatt caagaacgct ggattttcca
      541 aatagagcgc atcgccccgt tccataaacg cagtggcaaa aagcgcttgt gccgcaccca
      601 ccccttggcc gatgatgccg atgcgagagg tgtcgacttc tttacgcaaa cgcgccactt
      661 ccaacgcgcg gaatgcatcg agaaaaattt ttgccgcata gtattcagtc ggttcaagca
      721 agcgttcgcc aaaataaccg tatgcggcgg attgcttttc agttttttgc ttttctctat
      781 cgttctgcat ttccgctttt ctacccgttt gcttatctcc tggcacaggg cgcggcgcct
      841 cgtgcccccg catacgcaca atcatttgcg caatccctac gttcatcagt cccttataca
      901 ccaccgcctc tttgccgtaa tcgggaaaaa tcaaaacgag cggcggtttt cctgacattt
      961 tacgcggtac aaaaagttgc gcttggcaat ggtatttatc gatactctga aaatcgatga
     1021 gcaaattttg ttctgtcaaa attttacgtg aaaccttttt cttagcttcc atctcaatcg
     1081 gaattttttt taagcgagcg atttgcgctt tccagaattt atctaaatcg ggaggagcaa
     1141 gcgttgtatt tgcttcgcta ttaaagcaat cgtcgaataa ggccattggg agacatttat
     1201 atttttaagt atcgggcaaa gctcttttgt ctaacccacg gacatacctg acaaatgcta
     1261 cgccgcaatt ggggggt
//
LOCUS       DAOMPD010000665         1267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38469_length_1267_cov_1.811056, whole genome shotgun sequence.
ACCESSION   DAOMPD010000665 DAOMPD010000000
VERSION     DAOMPD010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38469_length_1267_cov_1.811056"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLY93_15260"
     CDS             complement(<1..1097)
                     /locus_tag="PLY93_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRP70881.1"
                     /translation="MTNNDNHSKASAAGLIIALGIIYGDLGTSPLYVFSAITASRLIS
                     EELILGGLSCIFWTLTLITSVKYVLVTLRADNHGEGGIFSLYTLVRRRGRWLIIPTVI
                     GGCALLADGMITPSITVSSAIEGLEALVPGIPTVKIVIVILSALFLFQQFGTNIVGRW
                     FGPVMLVWFLMIATLGVVHLVQNPAVLKALNPWYAYNLLVNYPKGFWILGAVFLCTTG
                     AEALYTDLGHCGLKNIRISWMFVKITLLLNYFGQASYLLTLSGQTFDANGRTALGLTP
                     FYSLMPEWFLPVGIFIATCAAIIASQAIISGAFTLIAEAIRLNLWPKVRIKYPSVLKG
                     QIYVPSANRILWIGCCAVTLYFQQASRMEAA"
BASE COUNT          371 a          316 c          304 g          276 t
ORIGIN      
        1 tacgccgctt ccatacgcga agcctgttga aaataaagcg ttaccgcgca acaaccaatc
       61 cacaaaatac gattggcgct gggaacataa atttgtcctt taagcaccga cgggtacttg
      121 atgcgtactt tcggccaaag gttgaggcgg atggcttctg caatgagagt gaatgcaccg
      181 ctgatgattg cctgcgaggc gataatcgca gcgcacgtcg ctataaaaat ccccacgggt
      241 aaaaaccatt caggcatcag tgaatagaat ggtgttaatc ccaacgcggt acgcccattc
      301 gcgtcgaatg tttggccact caacgtcaac aaatacgatg cttgaccaaa ataattgaga
      361 agcagggtta tcttgacgaa catccaactg atgcgaatgt ttttcaaacc acaatgcccc
      421 aagtcggtat agagcgcctc cgcgccggtt gtgcaaagaa acacggcgcc taaaatccaa
      481 aatcctttgg ggtagttgac gagtaaatta tacgcatacc aaggattgag cgctttaagt
      541 acggctggat tttgcaccag atgaacgacg cctaaagttg caatcatcaa aaaccatact
      601 aacatcacag gaccaaacca gcgccccacg atgttggtgc cgaattgttg aaaaagaaaa
      661 agcgcagaaa gaataacgat gacaattttt actgtgggaa ttccaggcac caatgcctcg
      721 agtccttcta tcgccgacga caccgtgatc gacggcgtga tcatcccatc ggcgagtagc
      781 gcacagccgc caatcacagt gggaataatg agccaacgcc cacgccgtct caccaaagta
      841 taaagagaaa aaataccgcc ttcaccgtgg ttatcggcac gcagcgtcac gaggacgtac
      901 ttcaccgagg tgataagcgt gagcgtccaa aaaatgcaag aaagcccacc caagataagc
      961 tcttcgctga tgaggcgcga tgcggtgatc gccgaaaaaa catataaggg ggatgttccc
     1021 aagtcgccgt agataatccc taaggcaata atgagccctg cggcagacgc tttggaatgg
     1081 ttgtcgttat ttgtcattgt attcccgtac gaacgttata gaggcgaatg cagtacatct
     1141 tccaagtttt tgagcgtcac ttcactgatg agtttccgct ttagctgcca cccgttcgcg
     1201 tggcggataa aaacaaaggt ttcgggaaaa cctgtaacgc gccatgcctt taagtcggtt
     1261 gcgcttg
//
LOCUS       DAOMPD010000666         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38495_length_1266_cov_2.094137, whole genome shotgun sequence.
ACCESSION   DAOMPD010000666 DAOMPD010000000
VERSION     DAOMPD010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38495_length_1266_cov_2.094137"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLY93_15265"
     CDS             <1..343
                     /locus_tag="PLY93_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRP70882.1"
                     /translation="HEDPQVPKFGRSKDGMKMRAGMVIAIEPMINAGTYDVFTDSDKW
                     TVRTKDGKLSAHFEHTVAVTSSGPVICTLPKGASINVFEMMGVENGYAEHADTAAEKQ
                     NHEARKAGVKV"
     gene            340..456
                     /gene="rpmJ"
                     /locus_tag="PLY93_15270"
     CDS             340..456
                     /gene="rpmJ"
                     /locus_tag="PLY93_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRP70883.1"
                     /translation="MKVRASVKKICPQCKVIRRAGVIRVICKANPRHKQRQR"
     gene            459..842
                     /gene="rpsM"
                     /locus_tag="PLY93_15275"
     CDS             459..842
                     /gene="rpsM"
                     /locus_tag="PLY93_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRP70884.1"
                     /translation="MARIEGIDLPNQKRVVIGLTYIYGIGPKVASEIMTKTGIPLTRR
                     VHELTEDEIAKIKKAIDGSFLVEGALRSKVQLDMKRLVDTGTYRGNRHRKGLPARGQR
                     TKTNARTRKGRRLTVAGKKSAPSKG"
     gene            852..>1266
                     /gene="rpsK"
                     /locus_tag="PLY93_15280"
     CDS             852..>1266
                     /gene="rpsK"
                     /locus_tag="PLY93_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRP70885.1"
                     /translation="MSEEVKDQAVAEEPKVNKKLKDKGRVPRGRVYINATFNNTIITI
                     TDMNGNSIAWASGGSLEFKGSRKATPYAAQLAARSALDKARERGLHEVEIYVKGPGVG
                     RDQAIRTLATSGLRVNLIKDITPVPHNGCRPRKKRR"
BASE COUNT          376 a          272 c          358 g          260 t
ORIGIN      
        1 gcatgaagac ccacaggtgc ctaagtttgg ccgtagcaaa gacggtatga aaatgcgtgc
       61 agggatggtc attgcgattg agccgatgat caacgctggc acgtatgatg tttttacaga
      121 ttcggataaa tggactgtgc gtacaaaaga cggcaagctg agcgctcatt ttgagcacac
      181 cgtcgcagtt acaagttcag gcccggtgat ttgcactttg cccaagggtg caagtatcaa
      241 tgtatttgaa atgatgggcg tcgagaatgg ttatgccgag catgccgata cggcggcgga
      301 aaagcaaaat catgaagcgc gtaaagcggg agtaaaagta tgaaggtgag agcatcggta
      361 aaaaagatat gtccgcaatg caaagtcatt cgtcgggcag gggtaatccg agtgatttgc
      421 aaagcaaatc cacggcataa acagaggcag aggtaattat ggcgcgtatt gaaggtattg
      481 acttacccaa ccaaaaacga gttgttattg gtttaacata tatttacggc attggtccaa
      541 aagtagcgag cgaaattatg accaagacgg gcattccgct gacgcggcgt gtacacgagt
      601 tgaccgaaga tgaaattgca aaaattaaaa aagcaatcga cggtagcttt ctcgtcgaag
      661 gtgcgctgcg cagtaaagtg cagttagata tgaagcgttt ggtcgacacg ggcacttacc
      721 gtggcaatcg gcaccgcaag ggtttacctg cgcgtggcca gcgtacaaaa acaaatgcaa
      781 gaacgcgcaa aggccgtcgt ttgacggttg cgggtaaaaa atcggcaccc agcaagggct
      841 aacggagtac tatgagcgaa gaagttaaag accaagcggt tgcggaagaa cccaaagtaa
      901 ataaaaaact caaagataaa ggccgcgttc ctcgtgggcg cgtctatatc aacgcgacgt
      961 tcaacaacac gatcatcacg attaccgata tgaatggaaa ttctatcgcg tgggcatcgg
     1021 gtggttcgtt ggagttcaaa ggctcgcgca aagcgacacc gtatgcagcg cagttggcgg
     1081 cgcgttccgc gttggataaa gcgcgcgagc gcggtcttca cgaagtagaa atttacgtaa
     1141 aaggccccgg cgtcggtcgc gaccaagcga tccgtacact ggcaacctct ggtctgcgcg
     1201 ttaatcttat taaagatatt acaccggtcc cgcacaacgg ctgcagaccg cgtaaaaaga
     1261 gaaggg
//
LOCUS       DAOMPD010000667         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38501_length_1266_cov_1.787779, whole genome shotgun sequence.
ACCESSION   DAOMPD010000667 DAOMPD010000000
VERSION     DAOMPD010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38501_length_1266_cov_1.787779"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLY93_15285"
     CDS             complement(<1..1033)
                     /locus_tag="PLY93_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRP70886.1"
                     /translation="MPSDRLLTAFEQLALIVGTPRALYPNDARFGDYLKDETYLTGAA
                     ELVLQPQSQKEVVAILQEVKALREKIPHEKKHLSLTLRGGGSGLSGAAVPSSGIVLDL
                     SHLKKIFEIDEKNLIIHAECGIILAELNQALADTSFYYAVDPSSSALCTLGGSIATNA
                     AGPSSLKYGTTRQQLASLHFLHTTGAIVQAGSIPNKTSMGFSLPDILCGSEGRLGIIL
                     DATVRLTPRAETTTLILAPFTSESDAIDFVFTLKQSGIRAKCIEFIDAYSLAVADFHS
                     QSGALLMIELDGFEKQVALEVERLIELAPTLHFVAAQSEKDRARLWTKRKSITGALKQ
                     RFAFKLGEDI"
     gene            1078..>1266
                     /locus_tag="PLY93_15290"
     CDS             1078..>1266
                     /locus_tag="PLY93_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70887.1"
                     /translation="MNLRRWVWIIFFSPLTLSHCLAFDRKLSPETCAPQRHKTDEIVV
                     SYSLPQEAHRILGEITVQY"
BASE COUNT          299 a          284 c          356 g          327 t
ORIGIN      
        1 cgatgtcttc tcccagcttg aacgcgaagc gctgtttgag cgcgccggtg attgacttgc
       61 gttttgtcca aaggcgagca cgatcttttt cgctctgcgc ggcgacaaaa tgaagagtgg
      121 gggctaactc tatcaggcgc tctacctcta acgccacttg tttttcaaag ccatcgagtt
      181 caatcatgag gagtgcgccg ctttgtgagt ggaagtcggc aacggcgagg ctgtacgcgt
      241 cgataaactc gatacacttg gcgcggatgc cactctgctt gagcgtaaag acaaaatcga
      301 tggcgtcgct ttcgcttgta aagggggcaa gaattaacgt cgttgtctct gcgcgtgggg
      361 tgaggcgtac ggtggcgtcg agaataatcc cgaggcggcc ttcgctaccg cagagtatat
      421 cggggaggga gaatcccatc gaagttttat tggggatgct tcctgcttga acaatggcgc
      481 ccgttgtgtg taaaaaatgc agcgaagcga gttgttgccg cgtggtcccg tatttaagcg
      541 acgagggacc tgcggcgttg gtggcaatcg aaccacccaa cgtgcaaagc gccgacgacg
      601 atggatcgac ggcataataa aaagaagtat ccgcgagagc ttggttgagt tcagctaaaa
      661 taatgccaca ttcggcgtgt ataattaaat ttttttcgtc gatttcaaag atttttttaa
      721 gatgcgataa gtcaagcaca atcccacttg aaggaaccgc tgcaccggaa agtccgctgc
      781 caccgccgcg cagtgtgagt gagagatgtt ttttttcgtg tggtattttt tcgcgtaatg
      841 ctttgacctc ttgcaaaatg gcgacaactt ctttttgcga ttgcggttgc agaacaagct
      901 ctgccgcacc ggtgaggtat gtttcatctt ttaaataatc accaaagcgt gcatcgttgg
      961 ggtatagggc gcggggggtg cccacaatga gggcaagttg ttcgaaggcg gttaaaaggc
     1021 gatcgctcgg catttggttg tcatggtgtg tcgacgagca agtacgcaaa gcgaaagatg
     1081 aatttacgtc ggtgggtttg gataattttt ttctctcctt taactttgag tcattgcctt
     1141 gcgttcgata gaaaattatc ccccgaaacc tgcgcccctc agcggcacaa aacggatgaa
     1201 attgtggtga gttattcgct gccgcaagaa gcgcaccgca ttttaggaga gatcaccgtt
     1261 caatac
//
LOCUS       DAOMPD010000668         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38836_length_1257_cov_2.531614, whole genome shotgun sequence.
ACCESSION   DAOMPD010000668 DAOMPD010000000
VERSION     DAOMPD010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38836_length_1257_cov_2.531614"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1257
                     /gene="lepA"
                     /locus_tag="PLY93_15295"
     CDS             <1..>1257
                     /gene="lepA"
                     /locus_tag="PLY93_15295"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRP70888.1"
                     /translation="YILPVINKIDLPSADVEKTKKQIAESLGLNPDDAVAVSGKSGIN
                     VEALMQAIIDKIPAPELKVQDALGHAAPRMASGDLPARALVFDAYFDAYRGVVEKIRV
                     FDGEIKKGDQIYFMRKGVTHPVNEIGISQLKLVPTPSLKAGEVGYIVTGIKNVADVML
                     GDTITLAAKRATEPLFKYKEVKPMVFSGLFPVNGDDYPLLKESMDKLALNDPALIYEP
                     ESSEALGFGFRVGYLGLLHMEIVQERLEREFDLALLTTAPSVTYHIYFQHKPMMTLNN
                     PAEFPDPGEIDRIEEPYVKVSIISPSEYMGAILQLLADKRGENVSLNYLDPTTVQIIY
                     LVPLAEMIFEFYDRLKSVSRGYASLDYEFSEYRKSALVKLDILVHANRVDALSMIVHK
                     NAAELRGRQVIEQLKEHIAKHQFQIA"
BASE COUNT          331 a          307 c          318 g          301 t
ORIGIN      
        1 ctatattcta cccgtcatca ataaaatcga tttaccctcg gcggatgttg aaaaaacaaa
       61 aaaacaaatc gccgagtcgt tgggattaaa ccccgacgat gcggtggcgg tttcggggaa
      121 atcggggatt aatgtcgaag cgttgatgca ggcgattatt gataaaattc ctgctccaga
      181 gttaaaagta caggatgcac tcggccacgc ggcgccacgg atggcaagtg gcgatctccc
      241 cgcacgcgcg ttagttttcg acgcgtactt cgatgcgtac cgtggcgtgg tcgaaaagat
      301 ccgcgtgttc gacggcgaga tcaaaaaagg cgatcaaatt tattttatgc gtaaaggggt
      361 gacgcaccct gtcaatgaaa ttggcatttc tcaactgaag ctcgtaccca caccgtcgtt
      421 gaaagcaggt gaggtggggt atatcgtcac tggcattaaa aacgttgcgg atgtaatgct
      481 cggcgacaca attaccctgg ctgcgaaacg cgccaccgag ccgctcttta aatataaaga
      541 agtaaagccg atggtgttct ccggcctttt tccggtgaac ggcgacgatt acccgctcct
      601 caaagaatcg atggataagc tcgcgctcaa cgaccccgcg ctcatctacg aaccggagag
      661 ttcagaagcg ttaggctttg gtttccgggt gggctacttg ggacttttac acatggaaat
      721 tgtgcaagag cgtttagagc gcgaatttga tttagccctc ctgacgactg cgccatcggt
      781 gacgtaccat atctatttcc agcacaagcc gatgatgacg ttgaataatc cggcagagtt
      841 ccccgatcca ggggaaattg atcgtataga agaaccatac gtcaaagtgt cgatcatttc
      901 accttcggaa tacatgggag caattttgca actgttggcc gacaagcgcg gtgaaaatgt
      961 ttcgctcaac tatcttgacc ccaccaccgt acaaattatt tatttggtgc cgctcgccga
     1021 aatgattttc gagttttacg atcgcttgaa aagcgtctcg cgcggttacg cgagcctgga
     1081 ttacgaattt tccgagtacc gcaagagcgc tcttgtaaag ctcgacattc tcgtacacgc
     1141 aaaccgcgtc gatgcgcttt caatgattgt gcataaaaat gcggcagagt tgcgcggtcg
     1201 gcaagtgatc gagcagctca aagaacacat cgctaaacac caattccaga ttgccat
//
LOCUS       DAOMPD010000669         1256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38876_length_1256_cov_2.393838, whole genome shotgun sequence.
ACCESSION   DAOMPD010000669 DAOMPD010000000
VERSION     DAOMPD010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38876_length_1256_cov_2.393838"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLY93_15300"
     CDS             complement(<1..389)
                     /locus_tag="PLY93_15300"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70889.1"
                     /translation="MVVQKLNTSEKKFHRLTRDNYPRIVALLKKLGAARRFILLKRAL
                     HALHPTSLALLWDSFDETQREIILSQLASEYAAEFLTELDSDERTAIFRSKDTAWIFE
                     RLEELVTDDTANILRELNVRDTNFILRR"
     gene            complement(371..814)
                     /locus_tag="PLY93_15305"
     CDS             complement(371..814)
                     /locus_tag="PLY93_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRP70890.1"
                     /translation="MAQQSADNAPLKTPKVDESLNQDSRLASASWVRLFFVLAGSLSL
                     ALGIIGIVTPILPTTPFILLSGFCFARGSTRFHKWILNHKYFGPMIHAFYHEKRIPLF
                     AKITATMMIASTMTATAFIVRKPIPIIGMATVGCGVLIYIWSFKN"
     gene            complement(818..>1084)
                     /locus_tag="PLY93_15310"
     CDS             complement(818..>1084)
                     /locus_tag="PLY93_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRP70891.1"
                     /translation="DMSISRKFREEVAKFLEKKPSVVVFDMNETEYLDSSALGALVTI
                     LRDVKNYNGEIRLANLNQTLKTLFKLSKLESMFKIFETVEDATK"
     assembly_gap    1085..1094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          365 a          247 c          283 g          351 t
ORIGIN      
        1 cgccgcagaa tgaaattggt gtcacggacg ttgagttcgc gtaaaatatt agcagtatcg
       61 tcggttacga gttcttccaa gcgctcgaaa atccacgcgg tatctttgct gcgaaaaatg
      121 gcagtccgtt cgtctgagtc aagctcggtc aagaactcgg cggcgtattc tgaggcaagc
      181 tggcttagaa taatttcacg ttgggtttcg tcgaaagaat cccataaaag cgcaagcgag
      241 gtgggatgca aagcgtgcaa ggcgcgcttg agaagaataa agcggcgcgc tgcgcccaat
      301 ttctttagca gcgcgactat tcgtggatag ttatccctcg tcaatcgatg gaattttttt
      361 tcactcgtgt ttagtttttg aacgaccata tataaataag cacaccgcaa cccacggtgg
      421 ccatgccgat gataggaatt ggtttacgca cgatgaaagc tgtcgctgtc atcgtgctcg
      481 caatcatcat cgtggcggta atttttgcaa aaaggggaat acgcttttca tggtagaaag
      541 cgtggatcat gggaccaaag tatttatgat ttaaaatcca tttatgaaaa cgtgtcgatc
      601 cccgcgcaaa acaaaaaccg ctcaaaagaa taaacggagt tgtgggtagt ataggagtaa
      661 ctataccgat gataccaagc gccaaagaaa gggatcctgc taagacaaaa aatagacgta
      721 cccatgaagc gcttgctaga cgagaatctt ggtttagtga ttcatccact ttgggtgttt
      781 taaggggcgc gttatcggcg ctctgttgcg ccatggtcta cttggttgcg tcttctacag
      841 tttcaaatat cttaaacata gactctaact ttgaaagttt gaaaagtgtc tttagggttt
      901 gatttagatt agcaagacgg atttcaccat tgtaattttt aacatcgcgc aaaattgtca
      961 cgagtgcacc taatgccgac gagtcaagat actcggtttc attcatatca aaaacgacga
     1021 cgctgggttt tttttcgaga aatttcgcga cttcttcacg aaacttgcga gaaatagaca
     1081 tatcnnnnnn nnnnttgacg aataggatta cacctttctc aaaaggttta tcgacaataa
     1141 gcatactgtc aagcttacta actgacggta gcgtcaatta aaaaaccttt ttttgtaaaa
     1201 actgcacttt tttttcaaaa agtgagaaaa attaagcgtt ttctggtatt tataat
//
LOCUS       DAOMPD010000670         1253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_38997_length_1253_cov_2.246244, whole genome shotgun sequence.
ACCESSION   DAOMPD010000670 DAOMPD010000000
VERSION     DAOMPD010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38997_length_1253_cov_2.246244"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLY93_15315"
     CDS             complement(<1..273)
                     /locus_tag="PLY93_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70892.1"
                     /translation="MRDQVKLLLPKVVREKIVIEERATKPPTRLDHFVREVERGGWGQ
                     TSKIEMIQLLDTLGHAYEVDGDWYFSVRADARFGSLSQLPEDEMLRL"
     gene            178..657
                     /locus_tag="PLY93_15320"
     CDS             178..657
                     /locus_tag="PLY93_15320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRP70893.1"
                     /translation="MVEAGRGLGRPLLDDNFLTHNFGEQQFDLITHWATLEHLPDAKK
                     FLQKMASLLAPEGKIYLSTCNTGYFARRYANAWRYLNVPEHVFYFNKKSLTHLAHSVS
                     LKINRAFSYGSGFTAIENSNLYYRLRKRFFDQMARFLLSGDMIVIELTHGASAPFAQ"
     gene            complement(676..>1253)
                     /locus_tag="PLY93_15325"
     CDS             complement(676..>1253)
                     /locus_tag="PLY93_15325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRP70894.1"
                     /translation="ALTGTARTYAADLSDPKIADGVLTRAIQENGPVDILINNAGVQH
                     IDHFTKITNETAQQLLNLNYSTPAQLMRQVLPSMVERDTGIVINTASLGAITPTPYMA
                     EYCASKAALAALATALSGEYKDSKIHFITVYPGRLKRPWRIMPMSVMKKARWITSPLA
                     QRKGWLKKFAMRLKKRNTKLCIQHFTKAQNL"
BASE COUNT          292 a          331 c          304 g          326 t
ORIGIN      
        1 caggcggagc atctcgtctt ctggcagctg cgagagcgag ccgaaccggg cgtcggcgcg
       61 gaccgagaag taccagtcgc cgtccacctc gtaggcgtgg ccgagcgtgt ccagcagctg
      121 gatcatctcg atcttgctgg tttggcccca tccgccgcgc tccacctcac gcacgaaatg
      181 gtcgaggcgg gtagggggct tggtcgccct ctcctcgatg acaattttct cacgcacaac
      241 tttggggagc agcaatttga cctgatcacg cattgggcaa cgcttgaaca tttacccgat
      301 gcaaaaaaat tcctacaaaa aatggcgtcg ctccttgcgc cagaaggcaa aatttattta
      361 agcacttgca ataccggtta tttcgcccga cgctatgcca acgcttggcg ttacctcaac
      421 gtgccggagc atgttttcta tttcaataaa aaatcactca ctcatttagc gcacagtgtc
      481 tcgctaaaaa taaaccgtgc attttcttat ggctcgggtt ttacagcgat tgagaacagt
      541 aatctatatt accgcctgcg taaaagattt ttcgaccaga tggcgcgttt tcttttaagc
      601 ggcgatatga ttgttatcga gcttacccac ggggcttcgg cgccatttgc gcagtaatcc
      661 aagtggctaa accgttcaca agttctgcgc tttggtaaaa tgctggatac acaactttgt
      721 gtttcttttt ttcaaccgca tagcgaattt ctttagccaa cccttccgct gtgccaaagg
      781 ggatgtaatc catcgcgcct ttttcataac gctcatcggc ataatccgcc atgggcgttt
      841 taatcggccc ggataaacgg taataaaatg aatttttgaa tctttatatt cgcccgataa
      901 agcagtggcg agcgccgcca gcgccgcttt cgatgcacag tattccgcca tataaggcgt
      961 gggggtaatc gcacccaaac ttgccgtgtt gataacaatc cccgtgtcgc gctcgaccat
     1021 cgacggcaat acttggcgca tcaactgcgc tggggtcgag taattgagat tcaacaactg
     1081 ttgcgccgtt tcgtttgtaa ttttagtaaa gtggtcgatg tgctgcacac cggcattatt
     1141 gatgaggata tcgacaggcc cattttcttg aatcgcccga gttaaaaccc cgtcggcaat
     1201 tttgggatca gataagtcgg cagcatacgt acgcgccgtg cctgtcaacg ccg
//
LOCUS       DAOMPD010000671         1250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_39104_length_1250_cov_3.071967, whole genome shotgun sequence.
ACCESSION   DAOMPD010000671 DAOMPD010000000
VERSION     DAOMPD010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39104_length_1250_cov_3.071967"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1250
                     /locus_tag="PLY93_15330"
     CDS             <1..>1250
                     /locus_tag="PLY93_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRP70895.1"
                     /translation="ILGYLFFQFSLNGLAFQYLWPQFPTWANLAIPFSILSGGALANQ
                     FTRSFLQLEKNAKRLNRIFIALIFLGGLTAITTLFLSYKIGILTAILYIFVSAIFMVT
                     AGITTLLQKFRPARFYVYSWAAFLAGIIFYVLKTVNILPAFFLTEYAIQIGSAIQVVL
                     LALGLADYINKLQNERKDLILDKLESRLEMMESVSRFVPERFLDFLGKKDISDISIGE
                     ATQRDMTLMFVDIRKFTTLSEHMTVEENFHFLNAYLQRMGPSIHRHRGIIDKFIGDAI
                     MALFPDDPEDALKAAIGMMQELKIYNDHRAQSHYDALQIGIGLHYGSLMLGTVGSSDR
                     MDTTVIGDTVNLAARIESVTKIFKAPIILSDFVYKKLADPSKYHLREIDSVRVRGKEH
                     PVVLYEAMDYIHPEILARRIESFPS"
BASE COUNT          311 a          295 c          270 g          374 t
ORIGIN      
        1 attttaggct atttgttttt tcagtttagc ctcaacggtt tggcgtttca gtatctgtgg
       61 cctcaattcc ctacatgggc aaatctcgct ataccgtttt ctatactttc ggggggcgct
      121 ctcgccaacc aattcacgcg ctctttttta cagcttgaaa aaaatgcaaa acggttaaac
      181 cgtattttta tcgcgcttat ttttttggga ggattgaccg ccataacgac gctttttctc
      241 tcttacaaaa ttgggattct caccgcaatt ctttatattt ttgtcagtgc gattttcatg
      301 gtgacggctg ggattactac cttgctgcaa aagtttagac ccgcgcgttt ttatgtctat
      361 tcatgggcag catttttagc tggcattatt ttttatgtgc taaagacggt gaacatactg
      421 cccgcttttt ttctcaccga gtacgccata caaatcggtt cggcgattca ggttgtactt
      481 ttggcgttgg gtcttgccga ctatatcaac aagttacaga acgaacgcaa ggatttaatc
      541 ctcgataaat tggaatcacg gctggaaatg atggaatcag tgtcgcgctt tgttcccgaa
      601 cgctttcttg attttttagg caaaaaagat atttccgata tttccatcgg cgaagccaca
      661 caacgcgaca tgacactgat gttcgtcgac atccgaaaat tcacaacgct ctccgaacac
      721 atgaccgtcg aggaaaattt tcattttttg aatgcatatt tgcagcgcat gggaccgtcg
      781 atacaccgtc atcgcggcat catcgacaaa ttcatcggcg atgcaatcat ggcacttttt
      841 cccgacgatc ccgaagatgc actcaaggcc gctatcggta tgatgcaaga gctcaaaatc
      901 tataacgatc accgcgccca aagccactac gatgcgttgc aaattggtat cggcttgcat
      961 tacggttctc ttatgctggg tacggtcggc tcatccgacc gcatggacac gacagttatc
     1021 ggcgacacgg taaacttggc agcgcggatt gagtcggtga caaaaatttt taaagcaccg
     1081 attattcttt ctgactttgt ttacaaaaaa ctcgccgacc ccagtaaata ccatttgcgc
     1141 gaaattgatt cggtgcgggt gcgcgggaaa gaacaccccg tggtgcttta cgaagcgatg
     1201 gattatatcc accctgaaat tttggcgaga cgcattgaat catttccttc
//
LOCUS       DAOMPD010000672         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_39199_length_1248_cov_2.697402, whole genome shotgun sequence.
ACCESSION   DAOMPD010000672 DAOMPD010000000
VERSION     DAOMPD010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39199_length_1248_cov_2.697402"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..741)
                     /gene="rho"
                     /locus_tag="PLY93_15335"
                     /pseudo
     CDS             complement(1..741)
                     /gene="rho"
                     /locus_tag="PLY93_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
BASE COUNT          296 a          305 c          291 g          356 t
ORIGIN      
        1 cgctttttca atcaccattt ctgtcacttg tacgtggcgg tttgcgggct catcaaacgt
       61 cgacgcaatc acttcaccgc ggacgctgcg cgccatatcg gtgacttcct cggggcgctc
      121 gtctatcaaa agcaccataa gcgtcacctc tggatggttt gtcgtaattg cgttggcgat
      181 gtcttgcatg agcatcgttt ttcccgtacg cggaggtgca acgatgagac cgcgctggcc
      241 tttaccaatc ggtgaaacca aatcgacgac gcgcatcgag agtttttcag cagtcgtttc
      301 aagacgaatt tgttcgtcgg gataaagcgg cgtcaagttg tcaaagtgaa cgcggcgcat
      361 tgcctcaccc gcaggcacgc cgttgatggt gtcgactttg aggatagcaa aaaaacgttc
      421 gttgtcgcgt ggcatacgaa tttggccgta caccgtgtcg cctttacgca aagcaaacgt
      481 gcggatttgc gccggggaaa cgtagatatc gtctgggcca gctaaataat tatagctcgc
      541 cgaacgcaaa aaaccatatc catcgggcat cacttcgagc acaccctcgg caaaaacctg
      601 gcctgagtta tcggattgct tttgtaggat tgaaaaaatg agcgccggtt tcttgagatc
      661 tgcaggattt tcaaccccgg cagccaacgc catttcaaca agcgcgttca tcgatttatt
      721 tttaaactcg ctaatctcca tttttttacc attgccccca ccattgccgc cattgctcgg
      781 caaagccgtc tgtgagcgcc cttttgtgtg cgtgtgcgct ggagccgttt cgtcttggag
      841 gagcgtttca cgaggttcgt gcgcatactc tttcacagcg ccttcatcct ttttggattt
      901 aagtcttaat ttttgcttat cgtgcgtctc ttcaacaata ttcgttgttt cttcgtctgt
      961 ggattttttc cgagccatat atcctcttca tgtgcatcaa aataaattgt gatttattca
     1021 aataaaaagt agtcgttagt gagtagagtt tatcggaggg gactgtctaa aaagtcacca
     1081 gcgatgcaaa aataatacgt tatttgctta tattgagaac gagaaggcgg cgtaaagcaa
     1141 atactaaata aactctgcca acgctttgga gcctatgtgc caactcgctg aggcaatttt
     1201 tttgcaaatt tttgtacgct attttgcaca aattcctccg ccaaagcg
//
LOCUS       DAOMPD010000673         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_39635_length_1239_cov_2.206926, whole genome shotgun sequence.
ACCESSION   DAOMPD010000673 DAOMPD010000000
VERSION     DAOMPD010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39635_length_1239_cov_2.206926"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..587
                     /locus_tag="PLY93_15340"
     CDS             96..587
                     /locus_tag="PLY93_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70896.1"
                     /translation="MKRNKRSLMDAFAFALTAIFLASCTNGIDNKNNSQKFGVSFTTA
                     TFKAKNIFFQLYSTDGIDVNKNPTGTVVGWGVVSIDSANGTGTGSLRSTDGFNKLVPP
                     GTYYLYCLADLDNGIDYQTTAWTNPIPRTSGDQYTDMLNVVVGNSETTKQVVQTDFKY
                     AVP"
     gene            complement(590..>1239)
                     /locus_tag="PLY93_15345"
     CDS             complement(590..>1239)
                     /locus_tag="PLY93_15345"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRP70897.1"
                     /translation="KIIFSPRRLAKGVFAEPELTLALPARVTAATGIDAMTRLMEAYL
                     AKGWHPMADGIALEGLRMVARALPTATQNGSDLKARGEMLMASLMGAVAFQKGLGVVH
                     SCAHALSAVVDMHHGLANGVMLDHALAFNFAKVPERFEALSHAVGLSTAADFLPYIKE
                     LKTCLSLPANLAQAGVSVDKVNALVKVALADGCHLSNPAPVGEGDFKAIFLNALK"
BASE COUNT          329 a          334 c          272 g          304 t
ORIGIN      
        1 caaaaactat ggtttggcca aagattgtcc gacctccccg ccgagggtaa gttttttaca
       61 cttgacaaat cctctctttt tatttgaaaa taactatgaa acgtaataaa cgctccctca
      121 tggatgcttt tgctttcgca ctcaccgcaa tctttttggc ttcttgtaca aacggcatcg
      181 acaacaaaaa taattctcag aaatttgggg tttcatttac gacggctacc ttcaaagcaa
      241 agaatatttt ctttcaactc tactcaacag acggtatcga tgttaataaa aatccaacag
      301 gcacggttgt tggttggggt gtagtgagta tcgactctgc caatggcacg ggtacaggct
      361 ctctacgctc caccgatggc tttaataaac tcgtacctcc agggacgtac tatctctact
      421 gcctcgccga tttagataac ggtattgatt accaaacaac cgcgtggaca aatcccatcc
      481 ctcgtacatc gggcgatcag tacaccgata tgctcaacgt tgttgtgggc aacagcgaaa
      541 cgacaaaaca ggtcgttcag acagatttta agtacgccgt accttaaact tattttaacg
      601 cgttcaaaaa aattgcctta aaatcaccct cgcccaccgg cgccgggtta gacaagtggc
      661 agccatccgc aagtgcaact ttaactaacg cattgacctt atctacgctg acgcctgctt
      721 gagcaaggtt cgccggcaac gacaagcatg ttttgagttc ttttatatac ggtaaaaaat
      781 ccgccgctgt ggataatccc actgcatgcg atagcgcttc aaaccgctct ggtacttttg
      841 caaaattaaa cgcaagcgca tggtcaagca tcacgccatt cgcaagacca tggtgcatat
      901 cgaccaccgc cgaaagtgcg tgcgcgcacg aatgcacaac gccgagtcct ttttgaaacg
      961 ccactgcgcc catgagagac gccatgagca tctcgccgcg tgctttgaga tcgcttccgt
     1021 tttgggtggc cgtggggagt gcacgcgcca ccatacgcaa cccttcgagc gcaatgccat
     1081 cggccatggg atgccaacct ttcgccaaat atgcttccat aaggcgcgtc atcgcatcga
     1141 taccggtggc agcggtaaca cgcgctggca atgcaagcgt caactcgggc tcggcgaaaa
     1201 cccctttggc caaacggcgt ggcgaaaaga taatttttt
//
LOCUS       DAOMPD010000674         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_39781_length_1235_cov_2.278814, whole genome shotgun sequence.
ACCESSION   DAOMPD010000674 DAOMPD010000000
VERSION     DAOMPD010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39781_length_1235_cov_2.278814"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>212)
                     /locus_tag="PLY93_15350"
     CDS             complement(<1..>212)
                     /locus_tag="PLY93_15350"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP70898.1"
                     /translation="GRCGSEGPHTGRGKQWSVDDYLLCDLPTACEYIINETGAPKLHW
                     VGHSMGGILGFFYQIRHEASNLQSFT"
     assembly_gap    213..233
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<234..549)
                     /locus_tag="PLY93_15355"
     CDS             complement(<234..549)
                     /locus_tag="PLY93_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70899.1"
                     /translation="MTIERKKICIQTPDHWEIALYRYASTALPKKRPEDGRGTDSSTR
                     FGSAVGEITELHHGTLPKRKYPVLLLHGIASNHKVWDFGVPEFSFARHLASQGYIVYS
                     MDL"
     gene            complement(552..>1235)
                     /locus_tag="PLY93_15360"
     CDS             complement(552..>1235)
                     /locus_tag="PLY93_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRP70900.1"
                     /translation="IAMRAQGKVVIGTVGEVPARTQVEIRNDDGTKVLPKGEAGVIYV
                     KGPGVMRGYYKNDIASAKVLKDGWMNTGDIGVFTKSGQLAIRGRAKDTIVLRSGENLE
                     PVPIETLLAQHPLIEQAVVVGQDQKNLGVLIWPEYDKLVDAGYEVREFDLKNDLNQIS
                     ELIDIFKEITASLINEEHGFKNFERISHVRFLPQKLLVGEELTALIKVKRNVIHKKYK
                     DLIENMFKE"
BASE COUNT          288 a          324 c          272 g          330 t
ORIGIN      
        1 ggtgaaactt tgcaggttcg atgcttcatg gcggatttga taaaaaaatc ccaagatgcc
       61 ccccatgcta tgccccaccc aatggagttt aggcgcacca gtttcattga tgatatactc
      121 gcacgccgta ggtaaatcgc acaagagata gtcatccacc gaccactgtt taccgcgccc
      181 cgtgtgcgga ccctcgctac cgcagcgacc acnnnnnnnn nnnnnnnnnn nnngcaaatc
      241 catcgaatag acgatataac cttggcttgc caaatggcgt gcgaatgaaa actcgggtac
      301 gccaaaatcc cacaccttgt ggttcgaggc gataccgtgg agtaacaaca cgggatactt
      361 tcgtttgggt agcgttccgt ggtgtagttc ggtaatttca ccaactgcgg agccgaaccg
      421 agtcgaagag tctgtaccac gaccgtcttc gggtcttttt ttcgggagcg cagtcgaagc
      481 gtagcgatac aaagcaattt cccaatgatc gggggtttgg atacaaattt ttttgcgttc
      541 gatagtcata actattcctt gaacatattc tcaatcaaat ctttatattt tttgtgtata
      601 acgttccgct ttactttgat gagcgcggtg agttcttcac ccaccaataa tttttgcggg
      661 aggaaacgaa cgtgcgatat ccgctcgaaa tttttgaagc cgtgctcctc gttgataagc
      721 gatgcggtaa tttctttgaa gatgtcgata agctcagaaa tttgattcaa atcgttttta
      781 agatcgaatt cacgcacctc gtaccccgca tcgacgagct tgtcgtactc cggccaaatg
      841 agaactccca agtttttttg gtcttgtcca accaccaccg cttgttcaat caacgggtgc
      901 tgcgccagca atgtctcgat aggtaccggt tctaaatttt cgcccgaccg gaggacaatg
      961 gtgtccttcg cacggccgcg gattgcaagc tgaccgcttt tagtaaagac accaatatca
     1021 ccggtgttca tccaaccgtc ttttaagact tttgcgctgg caatatcgtt tttgtaatag
     1081 ccacgcatca cccccggccc tttcacatag atgacacccg cttcaccttt gggtaaaacc
     1141 tttgtgccat cgtcgttgcg gatctccact tgggtacgtg cgggtacctc tcccacagtc
     1201 cctatcacca ccttgccttg cgcccgcatt gcaat
//
LOCUS       DAOMPD010000675         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_40204_length_1224_cov_3.039350, whole genome shotgun sequence.
ACCESSION   DAOMPD010000675 DAOMPD010000000
VERSION     DAOMPD010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40204_length_1224_cov_3.039350"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..624)
                     /locus_tag="PLY93_15365"
     CDS             complement(145..624)
                     /locus_tag="PLY93_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70901.1"
                     /translation="MLSKIFRVYFVIIFPMPLSALAALSDRLRCEAHFFEAHFNLPKD
                     YDKSKDVWYMTPEMHRLFKEPPLASYSYFKKLESAIWNLQILKPVREKFDFLPYYELT
                     FLEARATSLGEVYYRFEQKIAEQSSVQFSVDTQKPDIRFMEHRGEFDIFLYLTCNPQ"
     gene            complement(614..1078)
                     /locus_tag="PLY93_15370"
     CDS             complement(614..1078)
                     /locus_tag="PLY93_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRP70902.1"
                     /translation="MNYFIDYFYWLIAGSALVLLEFFIPGLVVIFLGLGALATSGMLY
                     LGYIRDPYLTVVFFTLTSIFMLATIRRVVKKFYPSDSEKVESDEDKILIGQEAITIST
                     ISPDNFEGRVKYSGTTWPAKSKEGDIAEATLVEIVGRENINFIVRRKENRAL"
BASE COUNT          387 a          278 c          239 g          320 t
ORIGIN      
        1 cccgggtttt aagaccccct taaatgcccc cagtcgctcc aatgtcgcca aagtgcgcat
       61 cggtataacg cggaacatac gcatcagtag aataaacacc accaacgctg caattgtaaa
      121 taaaccttcc ataaggttaa ctatctattg aggattgcat gtcaaatata aaaagatatc
      181 aaattcgcca cggtgctcca taaaccggat atccggcttt tgagtatcga cagaaaactg
      241 cacgctgctt tgctctgcaa ttttttgttc aaaccgataa taaacctcac ccaagctggt
      301 cgctctggct tctaaaaaag tcaattcata atacggcaaa aaatcaaatt tttcacgcac
      361 cggcttcaaa atttgtagat tccagattgc actctcgagt ttcttaaaat aagagtagga
      421 cgctaacgga ggttctttga agaggcggtg catctcgggc gtcatgtacc acacatcttt
      481 gcttttatca taatctttag ggagattaaa atgcgcttca aaaaaatgtg cttcgcagcg
      541 cagacgatcg gagagcgccg caagcgccga caacggcatg gggaatataa tgacaaaata
      601 aactctaaaa attttagaga gcacgatttt ctttacgacg gacaatgaaa ttgatattct
      661 cgcggcctac aatttcgaca agagttgcct cagcaatatc tccttcttta ctcttcgccg
      721 gccacgttgt gcctgaatat ttaacgcgcc cttcaaagtt gtcgggagaa atcgtgctta
      781 tggttatcgc ctcttggccg atgaggattt tatcttcatc cgattccact ttttccgaat
      841 ccgaaggata gaattttttg actacacgcc ggatggttgc cagcataaaa attgaagtga
      901 gcgtaaaaaa tacaacagtc aaataaggat cacggatata gcccaaataa agcatccccg
      961 atgtcgccaa agcccccaga cctaaaaaaa tcacgacaag gccggggata aaaaattcta
     1021 aaaggactaa tgcactacct gctataagcc aatagaaata atcaataaaa taattcatgt
     1081 ttctagtatc agcgcagctg cgcctaaaat acctgcgttt tcgtctcccc gtgtaaagac
     1141 gaattttgct acactatgga aaaccgaaag agtgtgttgt atataatttt cacgaatttg
     1201 tggaagtaag agatcctcgg atgc
//
LOCUS       DAOMPD010000676         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_40300_length_1222_cov_2.209083, whole genome shotgun sequence.
ACCESSION   DAOMPD010000676 DAOMPD010000000
VERSION     DAOMPD010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40300_length_1222_cov_2.209083"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1222)
                     /gene="mgtE"
                     /locus_tag="PLY93_15375"
     CDS             complement(71..>1222)
                     /gene="mgtE"
                     /locus_tag="PLY93_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRP70903.1"
                     /translation="IANILRELNVRDTNFILRRFDKNYTGKIKQVLKYKEGTAGALMK
                     SDFLAVSRHATVKSIVSQFQKMAKQGSGKNRKSSTVKEEAQTSSTNPGHGKLEDMQFT
                     FVVDRDNKFLGYIPIRKLILEKPSARAAEIMLPAQVVVAPHEDQESVAQVFKNYNLLS
                     LPVVDSRGILLGQITVDDVVDVLEEEATEDILRLGGVTQDAPQSQSLMRMARGRFPWL
                     AINLGMQVLSAYIVTFFNETLAQVIILAPLMTVISGQGGNATMQSITVVVRSLALGQI
                     NARNIAKIISKEVAVSAMNGLLCGSLTAGVVYAYTGRPLVAFCIVFAMFCDMLFAGLV
                     GSTLPLILKAARIDPALASGPLGTTMADICGFSVFLGTATILLQRFGAL"
BASE COUNT          319 a          289 c          289 g          325 t
ORIGIN      
        1 ggctttcagc aataattttt tctaataccg atgatgaatt gtgtagcatg agaaatccta
       61 cttaaaaaaa ttataaagct ccaaagcgtt gtagtagtat tgtggccgta cctaagaaaa
      121 cagaaaaacc gcaaatgtct gccatcgttg tacccaaagg ccccgaggca agcgcggggt
      181 cgatgcgtgc ggcttttaag atgagtggta atgttgaacc gacaagcccc gcaaagagca
      241 tgtcgcagaa catcgcgaac acaatgcaaa aagcgacaag tgggcggcct gtgtatgcgt
      301 acacaacacc agcagtgagc gagccacaaa gaagcccgtt cattgccgaa acagccactt
      361 ctttagagat gattttagcg atattgcgtg cgttgatttg ccccaatgcg agcgagcgca
      421 ccacgaccgt aatcgactgc atagtggcgt tgccgccttg gccgctaatc accgtcatga
      481 gtggcgccaa aataattact tgtgcaagcg tttcgttaaa aaaggtaaca atgtacgccg
      541 ataaaacttg catccccaaa tttatcgcga gccatggaaa acgtccgcgc gccattcgca
      601 tgagcgattg cgattgtggc gcgtcttgtg ttaccccccc taaacgcaaa atatcttcgg
      661 tcgcttcttc ttcaagcacg tcgacaacgt cgtcgacggt gatttgccct aaaagtatgc
      721 cacgcgaatc gacgacgggt agactcaaaa ggttatagtt tttgaatact tgcgcaacgc
      781 tctcttggtc ttcatgcgga gcgaccacca cttgagcggg gagcataatt tctgccgctc
      841 ttgccgatgg tttctctaag atcaatttgc gaatagggat gtagcccaaa aatttattat
      901 cgcggtcgac cacaaaagta aactgcatat cttcgagttt gccatggcct ggatttgtcg
      961 aagaagtctg tgcttcttct tttaccgtgg atgattttct gtttttgccg cttccttgtt
     1021 ttgccatttt ttggaattgg ctgacaatcg acttcacggt ggcgtgtcga ctcaccgcca
     1081 agaaatctga cttcatgagc gcgccggctg tgccctcttt gtatttcaaa acttgcttaa
     1141 ttttgccggt gtaattttta tcgaaacgcc gcagaatgaa attggtgtca cggacgttga
     1201 gttcgcgtaa aatattagca at
//
LOCUS       DAOMPD010000677         1213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_40680_length_1213_cov_2.534542, whole genome shotgun sequence.
ACCESSION   DAOMPD010000677 DAOMPD010000000
VERSION     DAOMPD010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40680_length_1213_cov_2.534542"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1213
                     /locus_tag="PLY93_15380"
     CDS             <1..>1213
                     /locus_tag="PLY93_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRP70904.1"
                     /translation="GLSVSEEFGGSGASCLMTTVAHEAFAEGSLDGGLTLALGAHAII
                     GTMPIALLGTPEQKKKYLPKLASGEWTAGLGLTEPSSGSDAAGSMRTVAVRKGDRYII
                     NGSKMFITNGPIGDVFVCMAITDKKKGAFGVSVFIVEKDFKGFSVGKKLNKMGMKTST
                     TSELIFEDMEVPVENRISEENSGFLRVGRATLEWERTVLVAAGLGSMNGLIRQGTRYA
                     KQREQFGEPIIRFQAIQDKIARSRYKLDASRLLIYQSAIKKDMGLPAPIESSIGKLYT
                     TESSVEVTYDMGQIFGGYCFIHEYPVERAYRDVRLSTLGAGTSEVMRSIIAANMKELW
                     FDTDLNEEQKELFHAFRNFCQKEIAPHTAAVDKQGEIPRSHFEKLYQVGYNGLAHEEA
                     YGGSNSTYFMAT"
BASE COUNT          315 a          279 c          331 g          288 t
ORIGIN      
        1 gggtttatcg gtgtctgaag agtttggcgg tagtggtgcg tcgtgcctta tgaccaccgt
       61 tgcgcacgaa gcgtttgccg aaggttcgct cgatggtggg ctcacgctcg cgttgggtgc
      121 ccatgcaatt attggaacga tgccgattgc gcttttggga actcccgaac aaaagaaaaa
      181 gtacctaccc aaactcgcct cgggagagtg gacagcgggc ttggggctca ccgaacccag
      241 ctcgggctca gatgcggcgg gcagtatgcg cacagtggcg gtacgaaaag gcgaccggta
      301 tatcatcaac ggcagcaaaa tgtttatcac caacggcccc attggagatg tctttgtgtg
      361 tatggcaatt accgacaaaa agaaaggcgc atttggtgtt tctgttttta ttgtcgagaa
      421 agacttcaaa ggtttttctg taggtaaaaa actcaataaa atgggcatga agacttccac
      481 tacctctgaa cttatttttg aagacatgga agtccccgta gaaaatcgaa ttagcgaaga
      541 gaattcgggt ttcttgcgcg tgggtcgcgc cactctcgag tgggagcgca ctgtattggt
      601 ggcggcaggc ttgggatcga tgaacggcct catccgccaa ggcacgcgct atgcaaaaca
      661 gcgcgagcaa ttcggcgaac cgattatacg ctttcaagca attcaagata aaattgcacg
      721 cagccgttac aagctcgacg cgtcgcgact tctcatttac caaagtgcga ttaaaaaaga
      781 tatgggactc cccgcaccga ttgaatcgtc gatcggtaag ctctacacca ctgaaagcag
      841 cgtcgaagtg acgtacgaca tgggccagat ttttggcggt tattgtttta ttcacgagta
      901 tccggtggag cgcgcgtacc gcgatgtccg tttgtccacg ttgggcgctg gaacctcaga
      961 ggtgatgcgt tcgattatcg ccgctaacat gaaagagctt tggttcgata ccgacttaaa
     1021 cgaagaacaa aaagaacttt tccatgcgtt tagaaatttt tgtcaaaaag aaattgcacc
     1081 gcacactgcc gctgtggata agcagggaga aatcccccgt tcacattttg aaaagttgta
     1141 ccaagtgggc tataacggtt tagcgcatga agaagcgtac ggcggctcga acagcacgta
     1201 tttcatggcg acg
//
LOCUS       DAOMPD010000678         1199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_41277_length_1199_cov_2.868881, whole genome shotgun sequence.
ACCESSION   DAOMPD010000678 DAOMPD010000000
VERSION     DAOMPD010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41277_length_1199_cov_2.868881"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLY93_15385"
     CDS             <1..456
                     /locus_tag="PLY93_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70905.1"
                     /translation="ILIFRGEEGLGVRVGAQALKYLNLMVIAATLAFAANCKKPAAET
                     LAVPGDAKSAMTAGLKLMADSVPLLEKASDGASAAAIVNKIVAFSAAAEAKFPELKEL
                     DKHPELKAESEKYGEDAMKFLMAVATVSEKFKDSAELKAAGDKLVQVEK"
     gene            561..>1199
                     /locus_tag="PLY93_15390"
     CDS             561..>1199
                     /locus_tag="PLY93_15390"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRP70906.1"
                     /translation="MLRYKADILSVFYMIVTTALLIVNWNLPEFNGWLFLGSILMATS
                     CFAMAHNHNHIPMWRIDFLNKLTDYWLTLFYGFPVYAWIPTHNLNHHQYNNREGDYTI
                     TWRLSEKNNLLTMITFPFISAYYQQTPTTNFLKHYWRTNKTKFAYYISQYVVWGLWII
                     GAFYLDSRKALLYIAIPMIVSTQFVLIINYIQHVHTDEESQYNHSRNFTGFYN"
BASE COUNT          328 a          284 c          264 g          323 t
ORIGIN      
        1 attttgattt ttagggggga agaagggctt ggtgtgcgag ttggcgcaca ggctcttaag
       61 tacttgaatc taatggtaat cgcagcgaca cttgcttttg ccgctaattg caagaagccc
      121 gctgctgaga cattagcagt gccaggcgac gcgaagagcg ctatgaccgc aggattaaaa
      181 ctcatggcgg actctgttcc tctcttagaa aaagcttcag acggtgcatc cgctgcggca
      241 attgtgaaca aaattgttgc attttcagca gcagccgaag caaaatttcc agagctcaaa
      301 gaattggaca aacatcccga gttaaaagca gagagcgaga agtatggcga agacgcaatg
      361 aagtttctca tggcggtagc gacagtttct gaaaaattca aagacagtgc cgaactcaag
      421 gctgctggtg ataagctagt acaagtcgag aaataagccg atacatattg agtcgagggg
      481 cctgatttct cctcgattct ttatcccttg cgtatatcct cgatggtttg acgagctgtc
      541 tcatttttgc acactgcacc atgcttcgat acaaagcgga tatattgtcg gtcttctata
      601 tgattgtgac gacggcactt ctcatcgtca actggaacct tcctgaattt aatggatggc
      661 tcttcttggg atccatcctc atggcgacaa gctgcttcgc gatggcgcac aaccacaacc
      721 acataccaat gtggcggatt gactttttaa ataaactcac cgactattgg ctcacgcttt
      781 tttatggatt ccctgtgtat gcgtggattc ctacgcacaa cttgaaccac caccagtaca
      841 acaaccgcga gggggattac accattacat ggcgtctttc cgaaaaaaat aatcttttga
      901 cgatgatcac atttccattc atcagcgcgt actaccaaca aacgccgacg acgaattttt
      961 tgaagcacta ttggcgtacc aacaaaacca agtttgcgta ttatatttca cagtatgtgg
     1021 tgtggggtct gtggattatc ggcgcgtttt atttagattc acgcaaagct cttctttata
     1081 tagcaatccc catgattgtc tcaacccagt ttgtgctcat cattaactac atccaacacg
     1141 tccacaccga cgaggaatcg caatacaatc actcgcgtaa cttcaccggt ttttacaac
//
LOCUS       DAOMPD010000679         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_41502_length_1194_cov_2.000878, whole genome shotgun sequence.
ACCESSION   DAOMPD010000679 DAOMPD010000000
VERSION     DAOMPD010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41502_length_1194_cov_2.000878"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..996
                     /locus_tag="PLY93_15395"
     CDS             52..996
                     /locus_tag="PLY93_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70907.1"
                     /translation="MKPRALFAKLWDDQFALLPQGSHATISGGGISGLHLAEALTLKG
                     VRVTLYDLKKTGGVRIPLMRACQSEKPHSALWQRAAAFTREKFHTLPQAAFGIDVRNN
                     WFVINTRAYLRALRTRLQEQGVVFVHAEAPSSQSDLHFNASGAGGYMSPVTRVAAGYE
                     SYWGRLKSHEETALKEKAIPHYLRSATRAALVHTEIKPIGELCQDAHRVHPHARHAIF
                     YGERLTCRDRLPLVGFDTHTAALSYDVLREKFIREPFDVYARAQEFPFLFTAMGYHAF
                     TYAPYLASVVAKHLTGHARDNENLVCTLTPTRFLSRKQ"
     gene            993..>1194
                     /locus_tag="PLY93_15400"
     CDS             993..>1194
                     /locus_tag="PLY93_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70908.1"
                     /translation="MNQESASKITLFHTREADFRAVFVSASDAINAAQKQIGLHEHAL
                     YHFSEMVVCANMLSGLFQNTNDI"
BASE COUNT          283 a          324 c          301 g          286 t
ORIGIN      
        1 aggctttggc caaaagcgcg acatgctcgt aggtaccagg cttcagtaaa tatgaaaccg
       61 cgcgctctct ttgcaaaact ttgggatgac cagtttgcgc tgttgccaca aggctcacac
      121 gccaccattt ccggtggtgg aatttctggt ttgcatttag ccgaggcgtt aacgctcaaa
      181 ggtgtgcgcg tgacgcttta cgatttaaaa aaaacaggcg gcgtgcgtat cccacttatg
      241 cgtgcatgcc aaagtgagaa accgcacagc gcactatggc aacgcgcggc ggcgttcacg
      301 cgagaaaaat ttcatacgtt accgcaagca gcgtttggca ttgatgtgcg taataattgg
      361 tttgtgatta acacacgcgc gtatttgcgc gcgttgcgta ctcgccttca agagcagggt
      421 gttgtgttcg tgcacgcgga agctccttcg tcgcagtcgg atttacactt caacgcgtcc
      481 ggtgccggtg ggtatatgtc gcctgtaacg cgcgttgccg caggctatga atcgtactgg
      541 ggtcgactga aatcccacga agaaacggcg ctcaaggaaa aagcgatacc gcattattta
      601 cgctccgcta cgcgcgctgc gttggtgcat accgaaataa aacccatagg agagctatgt
      661 caagatgcgc acagagtcca tccgcacgca cgccatgcca ttttttatgg agagcgcctc
      721 acttgccgcg accgcttacc gctcgttggc tttgacaccc acaccgccgc gttgagttac
      781 gatgtcctgc gtgaaaaatt tatacgcgaa cctttcgatg tgtacgcgcg cgcgcaagag
      841 tttccttttt tgttcaccgc gatgggctac cacgcgttca cgtatgctcc ttatttagcc
      901 agtgtcgtcg ccaaacacct cacaggtcat gcgcgcgata atgaaaattt ggtatgcaca
      961 ctgacgccta cccgttttct ttcgcgcaag caatgaatca agaaagcgca tcgaaaataa
     1021 ccctcttcca cacgcgcgaa gccgattttc gggcggtttt cgtaagcgca agcgacgcca
     1081 tcaacgctgc acaaaaacaa attggcctgc acgaacatgc gctctatcat tttagcgaga
     1141 tggtcgtttg tgcgaatatg ctctccggcc tttttcaaaa caccaacgac atcg
//
LOCUS       DAOMPD010000680         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_41588_length_1192_cov_2.455585, whole genome shotgun sequence.
ACCESSION   DAOMPD010000680 DAOMPD010000000
VERSION     DAOMPD010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41588_length_1192_cov_2.455585"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1192)
                     /gene="aspS"
                     /locus_tag="PLY93_15405"
     CDS             complement(<1..>1192)
                     /gene="aspS"
                     /locus_tag="PLY93_15405"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRP70909.1"
                     /translation="TLLSKAKPSPIPLDELDAEASEELRLEYRFLDLRRKKMQEALRL
                     RHEFTRAVRNYLDTEGFWEIETPILNKSTPEGARDFLVPSRLNAGEFYALPQSPQIFK
                     QLLMVSGAEKYYQIARCFRDEDLRRDRQPEFTQIDIEMSFVDKAMVMRLTQNMIQHAF
                     KSAFGVELDSEPIHISYHDAMELYGNDKPDIRFGMTLTELGDWAASTEFKVFRSAAES
                     GRLKALCVPGGGSLSRKDIDELTEWVKQDFKAKGLAWVKVSENGLDSLITKFLPEGAQ
                     QELLKRTQAKVGDIIFFAGDRADIVFATLSALRLRLAEKMKLIPEDVFKPLWVVDFPL
                     FDWDYETNSVISVHHPFTAPVPEDAHLVLEASHAAALGSADQKKLLGVRSNAYDFVLN
                     GVDIG"
BASE COUNT          298 a          297 c          305 g          292 t
ORIGIN      
        1 gccaatgtca acgccattca agacaaagtc gtatgcatta gaacgcacac ccaacagctt
       61 tttctggtcg gcgctaccca aggccgctgc gtgcgatgct tctaaaacaa ggtgtgcatc
      121 ctcgggcacg ggggcagtaa aaggatgatg cacgctgatg acagaattcg tttcgtagtc
      181 ccaatcgaag agaggaaaat caaccaccca caagggctta aaaacatcct cggggatgag
      241 tttcattttt tcagccaacc gtaacctaag cgccgagagg gtcgcaaaga caatgtcggc
      301 gcggtcgccg gcgaagaaaa taatatcgcc gactttcgct tgtgtgcgtt taagaagttc
      361 ttgttgtgcg ccttcgggta aaaatttggt gatgagtgaa tctaaaccgt tttcagaaac
      421 tttgacccac gcaagccctt tcgctttgaa atcttgcttg acccattctg tgagttcgtc
      481 gatgtctttg cgtgaaagcg agcctccgcc aggcacgcaa agcgctttta aacggcccga
      541 ttcggcagcc gaacgaaaca ctttgaactc tgtcgacgcc gcccaatcgc ccaattcggt
      601 gagagtcata ccgaatcgga tatcgggttt atcgttacca taaagctcca tcgcatcgtg
      661 gtaagaaata tggatgggct cggaatccaa ctcaaccccg aaagcgctct tgaacgcgtg
      721 ctggatcata ttttgcgtaa ggcgcatcac catcgcttta tcgacaaagc tcatttcaat
      781 atcgatttga gtgaactcgg gttggcggtc gcggcgtaag tcttcgtcgc ggaagcaacg
      841 cgcaatttgg taatactttt ctgctccaga aaccatgagc aactgtttga agatttgcgg
      901 cgattgcggc aacgcataga actcacccgc gttgagacgg ctggggacta aaaaatcgcg
      961 cgcgccttcg ggcgtcgatt tattcaagat gggggtctca atttcccaaa agccttccgt
     1021 gtctaaatag ttgcgtactg cgcgggtaaa ttcgtgccgg agccgcaacg cttcttgcat
     1081 ttttttgcgg cgcaagtcga gaaagcggta ttccaaacgc aactcttcgg atgcctcggc
     1141 gtcaagttcg tccaaaggga tgggggatgg tttggcttta ctcaataatg ta
//
LOCUS       DAOMPD010000681         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_41719_length_1189_cov_2.087302, whole genome shotgun sequence.
ACCESSION   DAOMPD010000681 DAOMPD010000000
VERSION     DAOMPD010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41719_length_1189_cov_2.087302"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLY93_15410"
     CDS             <1..258
                     /locus_tag="PLY93_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRP70910.1"
                     /translation="MNHSCDPNTWFEKDGSMSARRDIEAEEEITYDYATSENRSNFSL
                     ICRCGSPECRGTVRGNDFATNDKLQKRYGDHIMPHVRRETL"
     gene            559..>1189
                     /locus_tag="PLY93_15415"
     CDS             559..>1189
                     /locus_tag="PLY93_15415"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRP70911.1"
                     /translation="MDANIAAKVIDAALQKGTSFVDLYEEETRNAVVSLKEKKIENAT
                     AGIEYGIGLRLLFGTEVVYGFTGNGNEESLMRLLHALFMARGKTTSSVEMPFKMSLPT
                     ITHAPRMQEVHILPSEKGQAAKLTYLQYADQLAYRFSPHIQQVAVSMADSTKKICIIN
                     SEGLFVEDERTRARFSVSVTAANTEGERFVASESPGALSGFEFFENLDIE"
BASE COUNT          337 a          267 c          290 g          295 t
ORIGIN      
        1 atgaaccaca gttgcgatcc caatacgtgg ttcgagaaag acggatccat gagcgcgcgg
       61 cgcgacatcg aagcagaaga agaaattaca tacgactacg ccaccagcga aaaccgtagc
      121 aatttctcgc ttatatgtcg ttgcggaagc ccggagtgcc gagggactgt gcgcgggaat
      181 gattttgcaa ccaacgacaa gttacaaaaa cggtatgggg accacatcat gcctcacgta
      241 cggcgagaaa ctttgtagag ttttatcgtt gctttcgtga gcatccacga aaaattttta
      301 ttgtgtgaaa aaattccggc gtgtgtatcg cgtgcgagtt gcgcgatatg cgtatcgact
      361 tcttgcgctt caaacaagag tgtgtgggat aaatgcgcgg taacacggcg ggtttgaacg
      421 cgtcttgcgc gagtgttgac attttgtaca cacgctgctc tgagcaaccg tatgatgcaa
      481 catcaaaaga aataaatttt gtgaatatcc tcaaatttta aggttggcgt aaggccatct
      541 tcggtgtata ttacttgcat ggacgcaaac attgcggcaa aggttatcga cgctgctctc
      601 caaaaaggta caagctttgt cgatttatac gaagaagaaa cccgtaacgc cgttgtttca
      661 cttaaagaga agaaaattga gaacgccact gcggggattg aatacgggat tgggttaaga
      721 ttactctttg gcactgaagt ggtctatggt tttactggca acggcaacga agaaagtctc
      781 atgcgccttt tacatgcgct ttttatggca cgaggaaaaa caacatcgtc ggtagagatg
      841 cctttcaaga tgtctctgcc gacaataacg cacgcaccac ggatgcaaga agtacacatt
      901 cttccttccg aaaaaggaca agcggcaaag ctcacttatt tacaatacgc cgatcaactc
      961 gcgtacagat tttctcccca catccaacaa gtcgctgttt cgatggccga ctcgacaaaa
     1021 aaaatttgta tcattaatag tgagggactc tttgtcgagg acgaacgcac ccgcgcccgc
     1081 ttttcagttt ctgtaacagc ggcaaacacc gaaggcgagc gctttgtcgc gagcgaatct
     1141 ccgggcgcgc tttctggatt tgaatttttt gaaaaccttg acattgaaa
//
LOCUS       DAOMPD010000682         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_42192_length_1179_cov_2.418149, whole genome shotgun sequence.
ACCESSION   DAOMPD010000682 DAOMPD010000000
VERSION     DAOMPD010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42192_length_1179_cov_2.418149"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1059
                     /locus_tag="PLY93_15420"
     CDS             136..1059
                     /locus_tag="PLY93_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRP70912.1"
                     /translation="MKLKFTSLFIALSMVTGIFAGAGSSQTSLKGSYPIVLSHGMFGW
                     GANDTNSIIGIMQYWGGLDDYLFSQGAVVYAPGKSALQSNAYRANELKTKINLFMASG
                     GYSKVHIIGHSQGGLDSRYMVANLGMKSKVSTLTTLSTPHYGSPVADIVLTVVPSWLM
                     PYVSTALNALVNVIWGGNKNNLKASLESLTTANAAAFNASTPNQSGVKYYSYGSKVTV
                     PDLIQHPMMGLLVPVTGIGGVVKGQGASNDGLVPLTSAKWGTWKGSPNYGFFTTGLDH
                     LEVSNTLHMGETWFDVKGFWMAMALNAKNNQ"
BASE COUNT          327 a          238 c          285 g          329 t
ORIGIN      
        1 tttgcaaaaa aattgcctca gcgagtgggc gcacgggctc taaattaaac tacgtgttgc
       61 atcgcgcaat tttttggcac tttttcctct tgaaacgaca taatatcata ataagacgtg
      121 aaggagaaat tgtctatgaa gttaaaattt acttcattat ttatagcgct ctcgatggtg
      181 accggtattt ttgcgggcgc gggaagctca caaacttcgt taaaagggtc ataccccatc
      241 gtattgtcgc acggtatgtt tggctggggt gcaaacgata ccaactcgat tattggcatt
      301 atgcagtact ggggtggttt ggacgactat ctattttcgc aaggtgcagt ggtttacgct
      361 ccgggtaaat ccgcgttgca atccaatgca taccgagcta atgagctaaa aacaaaaatt
      421 aatttgttta tggcttctgg tggttatagc aaagtgcata ttatcggcca ctcccaaggg
      481 ggactcgata gccgctatat ggtagccaat ttgggcatga aatcaaaagt ttctacactc
      541 acaacgttga gcacaccgca ttacggtagc ccggttgcag atattgtttt aactgtggtg
      601 ccatcgtggc tcatgcctta tgtttccaca gcgctcaatg ctcttgtgaa tgtcatttgg
      661 ggcgggaaca aaaacaatct gaaagcatct ttagaatcgc tcacgacagc taacgctgcg
      721 gcgtttaacg cttcgacccc caatcaatcg ggagtgaaat actattcgta tggttcaaaa
      781 gtaaccgtgc ccgatttaat ccaacacccg atgatgggcc tcttggttcc agtgacgggg
      841 attggtggtg tcgtaaaagg gcagggggct tcaaacgatg gtttggttcc attaacttca
      901 gcaaagtggg gaacctggaa aggaagtcca aactacggtt tctttacaac gggcttagac
      961 caccttgaag tgagcaatac attacacatg ggagaaactt ggtttgatgt gaaaggcttc
     1021 tggatggcca tggcattaaa cgcgaaaaat aaccagtagt aagttataag ttagtagtaa
     1081 attaatgcat taaaagtatc gtagcgcgtg gcgctacgat acttttgtta gttgttgccc
     1141 tgtcgggcaa attttcaata aaacacaggt atcacagtg
//
LOCUS       DAOMPD010000683         1159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_43135_length_1159_cov_1.686594, whole genome shotgun sequence.
ACCESSION   DAOMPD010000683 DAOMPD010000000
VERSION     DAOMPD010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43135_length_1159_cov_1.686594"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..710)
                     /locus_tag="PLY93_15425"
     CDS             complement(42..710)
                     /locus_tag="PLY93_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRP70913.1"
                     /translation="MPGFGESEKPARVTYTLQQFSEWITRFLDKMHCDHFILAGHTLG
                     GAIAAELALQIPSRVHRLILISAAGLVPTNDGDNIYERILNGQNPFEVNSYDDFRAFF
                     RLIFSNPNRAPLPLKNYLFMKFAANRTWYHKIFLDIMGDISNEDLIDKRKAEQVKKFE
                     SIRTPTLIVWGDRDGFFPESIGCWMHRLVKDSRYVVLPGAAHMPPMEQANKLSKLTLS
                     FLNA"
     gene            complement(622..1026)
                     /locus_tag="PLY93_15430"
     CDS             complement(622..1026)
                     /locus_tag="PLY93_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70914.1"
                     /translation="MTSTAPAMENQSHLLPHIDLEFWLNAAYMRFIPQSIRWNLGESL
                     YNVFLGARRKATGARLGTAFLGDITVKYLEWGRPQLPKLVILHGFSDSKPAFSPLPRH
                     RPRHPWVWRERKTSARHVHSPAIFGVDYPLSG"
BASE COUNT          291 a          259 c          321 g          288 t
ORIGIN      
        1 gtatgattgg tccccaaatt ggctcgaagc cgcgtgaaat tttaggcgtt caagaaactg
       61 agcgtaagct ttgacagttt atttgcctgt tccatcggtg gcatgtgcgc tgcgcccggc
      121 agcacaacgt aacgcgaatc tttaacgagg cggtgcatcc aacagcctat cgattcggga
      181 aaaaaaccat cgcggtcgcc ccacacaatg agagtggggg tgcgtatcga ttcaaatttt
      241 ttcacttgtt cggctttgcg cttgtcgatt aaatcttcgt tggagatatc gcccatgata
      301 tccaagaaaa ttttatggta ccatgttcga ttggcggcaa atttcataaa aagataattt
      361 tttaggggta aaggcgccct gttaggatta gagaaaatca ggcggaagaa cgcacggaag
      421 tcgtcgtaag aattcacctc aaacggattt tggccgttca agatacgttc gtagatgtta
      481 tcgccgtcgt tggtcggcac aagacctgct gcagaaatga ggattaagcg gtgtacgcgg
      541 ctgggtattt ggagcgctaa ctcggcggca atcgcaccgc ccaacgtgtg gcctgcgagt
      601 ataaaatgat cgcagtgcat tttatccaga aagcgggtaa tccactccga aaattgctgg
      661 agagtgtacg tgacgcgcgc tggtttttcg ctctcgccaa acccagggat gtcgggggcg
      721 atgacgtggt agcggcgaga aagcaggttt gctgtcagaa aaaccgtgta ggataactag
      781 tttcggcagt tgcgggcgcc cccactcgag gtatttgacg gtgatgtcgc ccaaaaaagc
      841 agtgcctaac cgtgcgccag ttgccttacg gcgggctcct aaaaagacat tgtagaggct
      901 ttcgcccaaa ttccagcgga tgctttgggg gataaaccgc atatacgcgg cgttcagcca
      961 gaactcaagg tcgatatgag gcaataaatg gctctggttt tccatagcgg gtgcggttga
     1021 agtcatccac cagccttttg acgggtgtca atccgtatag caaaatgaac gtttgttcat
     1081 ttggagaatg tgctgcggta tgtatatgtc taacccacgg acatacctga caaatgctac
     1141 gccgcaattg gggggtatt
//
LOCUS       DAOMPD010000684         1156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_43295_length_1156_cov_1.725704, whole genome shotgun sequence.
ACCESSION   DAOMPD010000684 DAOMPD010000000
VERSION     DAOMPD010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43295_length_1156_cov_1.725704"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..872)
                     /locus_tag="PLY93_15435"
     CDS             complement(60..872)
                     /locus_tag="PLY93_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRP70915.1"
                     /translation="MTMGYQFFLIEKKAQVATLWLNRPEKRNFMNWHFWEELPEAIAE
                     INNDDSIKVFIVAAKGASFSIGLDLPDFVERFYWLGDADGSAENNLRVEELVLTMQKG
                     MRALADSPKPSIAAVQRHCIGGGLDLVAACDIRYASSDVIFSLREVKVAIVADMGSLQ
                     RLPHIIGEGATRELALTGRDVGADEALRLGLVTRVLPDKEALYAAALKTAEEMASNPA
                     VVMRGVKYVLDKQRGMDIGRSLDFVALYNGSYLQSKEFVALVKGFAERKKKS"
     gene            complement(898..>1156)
                     /locus_tag="PLY93_15440"
     CDS             complement(898..>1156)
                     /locus_tag="PLY93_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70916.1"
                     /translation="RTPMNRAAFRATLTAVWLILNAALFVVYILNGTFTMHEGLTFLY
                     LLPTIPLALKVGGCLHTRVSEWWFRISLQSLLLLSAAALLF"
BASE COUNT          318 a          316 c          258 g          264 t
ORIGIN      
        1 aaaacgcact caacttgacc ccaaaagtgt cagttatctc tgtgggttgc atagacgcgt
       61 caacttttct tcttgcgttc agcaaatcct ttgacaaggg cgacaaattc tttcgactgc
      121 aaatagctac cgttatacaa cgcgacaaaa tccagagagc ggccgatatc cataccacgc
      181 tgtttatcca agacatattt aactccgcgc atcacgaccg ctgggtttga tgccatttcc
      241 tctgcagttt taagagccgc agcgtagaga gcttctttgt cgggcaatac tcgagtgaca
      301 agccccaagc gcaacgcttc gtccgcaccc acatcgcgtc ctgtgagcgc cagttcacgc
      361 gttgcgcctt cgccgatgat gtgcggcagg cgttgtagcg accccatatc ggcaacaatg
      421 gccactttga cttcgcgcaa tgaaaaaata acgtcgctgc tcgcgtaacg gatatcgcac
      481 gccgcaacta aatcaagtcc accgccgatg caatggcgtt gaaccgctgc aatcgacggc
      541 ttgggagaat cggcgagcgc acgcattccc ttttgcatcg tcagtaccaa ctcttccaca
      601 cgcaaattat tttcagcaga accatccgcg tcgccaagcc aataaaaccg ttcgacaaaa
      661 tcaggtagat ccaaaccaat cgaaaaactt gcccctttcg cggcgacgat aaaaactttt
      721 atgctatcgt cgttgttgat ttcagcgatg gcttcgggca attcttccca aaaatgccaa
      781 ttcataaaat tgcgtttttc gggacggttg agccagagcg tcgccacctg cgctttcttc
      841 tcgattagga aaaactgata ccccatagtg attatccctg cttaaagagt ctatgcgtca
      901 aaacaataac gcagcggcag aaagcagtaa gaggctttgt agactaatgc gaaaccacca
      961 ttcggagacc cgtgtatgca agcatccccc cactttaaga gctaaaggga ttgtggggag
     1021 taaatataaa aacgtgagcc cctcgtgcat agtaaacgta ccgttcaaaa tataaaccac
     1081 aaagagcgcg gcgtttaaga taagccacac cgccgtgaga gttgccctaa acgcagcacg
     1141 attcatgggc gtgcgg
//
LOCUS       DAOMPD010000685         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_43343_length_1155_cov_1.646364, whole genome shotgun sequence.
ACCESSION   DAOMPD010000685 DAOMPD010000000
VERSION     DAOMPD010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43343_length_1155_cov_1.646364"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLY93_15445"
     CDS             <1..1153
                     /locus_tag="PLY93_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70917.1"
                     /translation="GKKELDQTEVYTRALGVETRRYRELLGPRKVRGFDIGGGTPSFV
                     SAALLENYLATVNEDFSFTEDAEISIETTPRIASLEREKIFAYSAMGISRISMGVQVT
                     QPDLLKVLGRETNGVEHHFRARDHIRQAGFTKFNIDLMYGFAGQSDASWQATLEHAIR
                     LEPEYITLYRMRYKLTRISEQAQQVGIAQVRAQAKLAKDLLSQAGYTANPGKNTYSRV
                     ARDTGTSAYLTRRVVEGAPYLGLGLGAQTFTDTTISYNSGAAGKNLTPYLRDIDKGVL
                     PIQDLYYLPREHMMAKMVAVSFYFGEVNLAAFREKFGITLEEAYPNAVDYTLAEGFME
                     YTNSENGTELTIDHRSLSLTELGARHFNGVIALFFAPSVKQYLLNSRHG"
BASE COUNT          291 a          281 c          304 g          279 t
ORIGIN      
        1 cgggaaaaag gaactcgacc aaaccgaagt ctatacgcgt gcgctgggcg tagaaacacg
       61 ccgttaccgt gaacttttag ggccacggaa agtgcgtggg tttgacatag ggggaggcac
      121 tccgtcgttt gtttcggcgg cgttattgga aaattatctc gcaaccgtta acgaagattt
      181 ttcttttacc gaagatgctg agatctctat tgaaacgacg ccacgcatcg cttcactcga
      241 gcgcgaaaaa atttttgcgt atagcgcaat gggtatctca cgcatcagta tgggtgtgca
      301 agtgacgcaa cccgatttac ttaaagtttt agggcgcgaa acaaacggcg tcgaacacca
      361 cttccgtgcg cgcgaccaca ttcgtcaggc aggatttaca aagtttaata tcgacctcat
      421 gtatggtttt gccggccaat cggatgcatc gtggcaggca acgctcgaac atgcgattcg
      481 gctcgaaccg gaatatatta ctctctaccg catgcgctat aagttgacgc gtatttccga
      541 acaagcgcag caggtaggta ttgctcaggt gcgtgcgcag gcaaaacttg caaaagattt
      601 actgtcgcaa gcaggctaca ctgcgaaccc aggcaagaac acgtatagtc gtgtggcgcg
      661 cgacaccggc acgagtgcat acctcacacg gcgtgtcgtc gaaggcgctc catacttggg
      721 gttgggtttg ggcgcgcaga cttttaccga cacgacaatt agttacaata gcggcgcagc
      781 gggcaagaac ttaactccat acttgcgcga catcgacaag ggcgtgttac cgattcaaga
      841 cctctactat ctaccgcgtg aacacatgat ggcgaagatg gttgcagtga gtttttattt
      901 tggcgaagtg aacctcgctg cgtttcgtga aaaatttggt atcacgctcg aagaagctta
      961 ccctaacgcc gttgactaca ctcttgccga agggtttatg gaatacacca attctgagaa
     1021 cggcacagag ctgacaatcg accaccgttc gttgtcgctg acagaattgg gggcgcggca
     1081 tttcaacggc gttattgcac ttttttttgc gccatcggtg aagcagtatt tactcaactc
     1141 gcgtcatggt tgatt
//
LOCUS       DAOMPD010000686         1149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_43639_length_1149_cov_1.627971, whole genome shotgun sequence.
ACCESSION   DAOMPD010000686 DAOMPD010000000
VERSION     DAOMPD010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43639_length_1149_cov_1.627971"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLY93_15450"
     CDS             complement(<1..312)
                     /locus_tag="PLY93_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70918.1"
                     /translation="MLKLSRLRSTILIIAVSVLLLANCIGRATPPEQVKLTQQQNEVL
                     GKFARQVGADELNILLHDPQAEGIPFHVHTAPPAAPTHAAHLPGIPAGDLLYILANVN
                     ED"
     gene            complement(312..>528)
                     /locus_tag="PLY93_15455"
     CDS             complement(312..>528)
                     /locus_tag="PLY93_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70919.1"
                     /translation="SGGNYLDLRIGYQYQEYNTTLNRRGSLTETQTDASGNFKVTWSE
                     DFLKKTESTKLSQSGIVILLGYTAGLF"
     assembly_gap    529..538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<539..>1149)
                     /locus_tag="PLY93_15460"
     CDS             complement(<539..>1149)
                     /locus_tag="PLY93_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70920.1"
                     /translation="ADWGTSRQISIEQDMLRQASQSNSAKNISVPWVQNSAINYMVGI
                     EGLLGVNLEQLPGLKDVKALHGKRFLRVGFHAYMAPYYKTTTQTVSGPFSYYDALALT
                     PTRTYNGKIQVDENLLHVDPGISIFYFYEQGFSKRRFMPYAGVEVGLSLLYGKRKYTM
                     QSSPYNAVVNDGFNPSYNATYRVEANLQESIINDFGFRVMPAI"
BASE COUNT          278 a          285 c          272 g          304 t
ORIGIN      
        1 gtcttcgttg acgttcgcta aaatatagag tagatcaccg gcaggaattc ccggtaagtg
       61 cgccgcgtgg gtgggtgctg ccggcggcgc ggtgtgtacg tggaagggga tgccttctgc
      121 ttgggggtcg tggagtaaaa tattgagttc gtccgccccc acctgccgcg caaacttacc
      181 caatacctca ttttgttgct gcgtgagttt tacttgttcg ggaggagtcg cacgaccaat
      241 acaattcgcc aacagtaaaa ccgataccgc tattattaaa attgttgagc gcagtcgaga
      301 caattttaac atcagaacaa ccccgctgta tatcccagaa gaatcacaat accgctttgg
      361 gataattttg tactttcagt tttctttaag aaatcctcac tccatgtcac tttaaaatta
      421 ccgctggcgt cggtttgggt ttcagtgagc gaaccgcgcc gattaagcgt tgtgttatac
      481 tcttggtatt gatagccaat gcgtaagtcg agatagttcc caccggaann nnnnnnnnca
      541 atcgctggca tcactcgaaa accaaagtcg ttgataatac tttcttgcaa gttcgcctca
      601 acacggtacg ttgcattata agacggatta aacccgtcgt tcacgactgc attataagga
      661 gaggattgca tcgtgtattt acgtttcccg tacaaaagcg acaaccccac ttccacgccc
      721 gcgtacggca taaagcggcg tttggagaaa ccctgttcat aaaaataaaa gatgctgatg
      781 cccgggtcga cgtgcagcag attttcatcg acctgaattt tcccgttgta cgtgcgcgtg
      841 ggtgtaagcg ccaacgcgtc gtaataagag aaaggtcccg ataccgtttg tgtcgtcgtc
      901 ttgtaatacg gcgccatgta cgcgtggaac cccacacgca agaaacgctt cccatgcaac
      961 gctttcacat ctttgagtcc agggagttgc tctaaattca cgcccaaaag tccttcgatg
     1021 cccaccatgt agttgatcgc actgttttgt acccaaggca cgcttatatt ttttgcgctg
     1081 ttcgattggc tcgcttgacg caacatgtct tgctcaatgg aaatttgccg tgacgtaccc
     1141 cagtcggcg
//
LOCUS       DAOMPD010000687         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_43754_length_1146_cov_2.793767, whole genome shotgun sequence.
ACCESSION   DAOMPD010000687 DAOMPD010000000
VERSION     DAOMPD010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43754_length_1146_cov_2.793767"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..495
                     /locus_tag="PLY93_15465"
     CDS             151..495
                     /locus_tag="PLY93_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70921.1"
                     /translation="MLSLNPILIVLTTLSIIALAAFLYFSVLLHRSKDRTLRWRGIVK
                     TLSLNGAPNSLAAIYSRWFIAFVAVYILACVSVGYAVWHTDHSFLIVIFPLLFYYFFA
                     RYFLWEKADLID"
     gene            488..>1146
                     /locus_tag="PLY93_15470"
     CDS             488..>1146
                     /locus_tag="PLY93_15470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRP70922.1"
                     /translation="MTKNSNKNRFVVIDHTPSTNTWIKEHPEFHRPYFSLRAREQTQG
                     RGQYSRSWFSGGRDENLAFSIVLPIQRADLSCAPLILALVLHYFLSQYIETKIKWPND
                     ILCHGKKIAGILCETIPHNHSLILAGIGVNVNTKKFPTELTNTTSLALEGATPPLEQL
                     WRQCYRSIVRAWHKANFPLSVTLIRDYNDAAYTYVKRPEISSSPLTFQTLLPNGSALF
                     QS"
BASE COUNT          293 a          271 c          241 g          341 t
ORIGIN      
        1 tggtcgaaaa gaagagtaaa ttttgaggaa aagctctctt tcaggcgggc gggacatcgc
       61 ttttttacgc gacttaggcc atagagcctt ggagctatac aaacaagtaa actagttttc
      121 gcagcgtcaa aagctatcga aatggtattc atgctctcgt tgaatcctat tttaatcgta
      181 ttgactacgc tttcaattat cgcattggcg gcatttcttt atttttcagt tcttttacat
      241 cgatcaaaag accgcacgtt gcgttggcgg ggtattgtga aaactctttc tctcaacggc
      301 gcccccaact cgcttgcagc aatatactct cgctggttta ttgcatttgt tgcagtttat
      361 attttggcat gcgtcagcgt cggctatgcg gtttggcaca cagaccattc gtttcttatt
      421 gtgatttttc cacttctttt ttattatttt ttcgctcgtt attttctttg ggaaaaagcg
      481 gatcttattg actaaaaata gcaataaaaa tcgattcgtc gttatcgatc acacaccatc
      541 gacgaataca tggattaagg aacacccaga gttccatcga ccttattttt ctctgcgcgc
      601 acgcgaacaa acacagggaa gggggcaata ctcacgttcg tggttttccg gtgggcgcga
      661 cgaaaattta gcattttcaa ttgtgcttcc gatacaacgc gccgatcttt cgtgtgcgcc
      721 gctcattctg gcgctcgtat tacactattt tctatcgcag tacattgaaa ctaaaattaa
      781 atggccgaat gatattcttt gtcatggaaa aaaaatcgcg ggaattttat gcgagacaat
      841 accgcataac cattcgctca tcctcgcggg tatcggtgtg aatgtgaata caaaaaaatt
      901 tccgacagag cttacaaaca ccacttcgct tgctttggag ggcgccacgc cgcctttaga
      961 gcaactctgg cgccaatgct accgcagcat tgtgcgtgcg tggcacaagg caaactttcc
     1021 tttgagcgtg acgctcatcc gcgactacaa cgacgctgcg tacacgtacg tgaaacggcc
     1081 agaaataagt tcttctccgc taacattcca aactctcttg cccaatggaa gcgcgctatt
     1141 tcaatc
//
LOCUS       DAOMPD010000688         1144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_43864_length_1144_cov_2.508724, whole genome shotgun sequence.
ACCESSION   DAOMPD010000688 DAOMPD010000000
VERSION     DAOMPD010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43864_length_1144_cov_2.508724"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..>1144)
                     /locus_tag="PLY93_15475"
     CDS             complement(439..>1144)
                     /locus_tag="PLY93_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRP70923.1"
                     /translation="TLSVTPLDLYSQHSKSYPQKALILFLEILFLAASYFLMFTDAFV
                     PYFGSSFSGSVPGIDRRAAIFVFSTVVFLRMNFMIFFLLKRRIPWGEAFEVPLAFAVY
                     YLGFAFLVEAQPMAWRFLDLLGITLFAVGSYLNSASEWQRHAWKAEPENRGKLYTRGL
                     FSLSMHVNYFGDVLWVSGYAVLTLNIYSAAIPAMLFTFFAFYNIPKLDTHLAEKYGEA
                     FVAFAATTRKFVPWVY"
BASE COUNT          327 a          275 c          290 g          252 t
ORIGIN      
        1 attgaaggcg taaaacaaag taaatttgca atttatgcaa cccccagaga taactgacac
       61 ttttgcgtca atttgagggg ttctaacaac gctgggcgag ttggcgcaca ggctccttac
      121 cgctcgccgt ctatcgtcgt ttatttacgg catatcttga gtcctaaaat tcgtgtggcc
      181 acactcattg ctgaagtatt tgaccaggca atgtaccgcc cgctgatgca gtcgcagagg
      241 tttgtgatgc cgcttcaggg ctttggccac aaaaggcggg gtagtccggc gcggtcgaag
      301 aaaggattgc gcgcgcaggg ccattcgcat tttcattatt gaattttaat cggaattttt
      361 gtctggtgcc gttgcataga gtccatggat ctcacgatgc cgtgatggac ggcctcacgg
      421 cgatgtgtac gttcgttgtt aatataccca cggtacaaat ttgcgtgtcg tcgcggcaaa
      481 tgccacaaac gcctcgccgt atttttctgc gagatgtgta tccaactttg ggatattata
      541 gaaagcgaag aatgtaaaaa gcatggcggg aatcgccgcg gaatatatat tcaacgtcaa
      601 cacggcgtag ccgctcaccc aaagtacgtc gccgaaataa ttcacatgca tcgagagcga
      661 gaacaacccc cgcgtataaa gtttgccgcg attttcaggt tcagccttcc acgcatgccg
      721 ctgccattcg ctcgcgctat tgagatacga accgacggca aagagcgtaa ttcctaataa
      781 atcgagaaag cgccaagcca tgggctgcgc ttcgactaaa aaagcgaaac cgagataata
      841 aacggcaaac gccaatggca cctcgaacgc ttctccccac ggtatgcggc gcttcaggag
      901 aaaaaaaatc atgaagttca tgcgcaagaa gacgacagtt gaaaaaacga aaatggcggc
      961 acggcggtcg atacccggaa cgctgccact aaaagacgaa ccgaaataag gcacgaacgc
     1021 gtctgtgaac atcaagaagt acgacgccgc gagaaaaaga atctcaagaa ataagatcaa
     1081 agccttttgg ggataacttt tactatgctg cgagtagagg tcgagcggag tcacggacaa
     1141 agtc
//
LOCUS       DAOMPD010000689         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44193_length_1137_cov_3.134935, whole genome shotgun sequence.
ACCESSION   DAOMPD010000689 DAOMPD010000000
VERSION     DAOMPD010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44193_length_1137_cov_3.134935"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLY93_15480"
     CDS             <1..820
                     /locus_tag="PLY93_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRP70924.1"
                     /translation="AGPGVFRISYLIRNIQRSTAYEAVVDKSEKTLTLKAYTMIQNAS
                     GENFTKAEMEGTLGNPFEGALMQGESKKILSLQASDVQFTKKYFFDNALSPEVSLLYE
                     VLNEKSAGLPEIQFPYGKARVYQEDSQGSEAFLGEDWAQETGAGQKLSLKLGQAKEVR
                     VKRSVVKRKEEIVNLPIKNYRETIKFQIENFKDSAVPLTLVEHPGGEWIVDGIEVKEE
                     SGERDTLRENTVSVDVKDERIDTNNLRLHVKVPANGHERKKLNVYVKLVLKNRW"
     gene            872..>1137
                     /locus_tag="PLY93_15485"
     CDS             872..>1137
                     /locus_tag="PLY93_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRP70925.1"
                     /translation="MRSSRATTSFIFVTLLLDIIGFGLVIPVMPKMVARFTTNDIETN
                     NWYMVMTASYGIMQFFFSPILGALSDKFGRRPILLLSIVGLGCN"
BASE COUNT          332 a          248 c          283 g          274 t
ORIGIN      
        1 agcaggtccg ggcgtttttc ggatttcgta cttaatccgc aacatccagc gcagtactgc
       61 gtacgaagca gtggtggaca aaagcgaaaa gacactcacg cttaaagcgt atacgatgat
      121 acaaaatgcg tcgggtgaaa atttcaccaa agccgaaatg gagggcacgt tgggaaaccc
      181 gtttgaaggc gctttgatgc aaggtgagtc aaaaaaaatt ctctcgcttc aagcgtcgga
      241 tgtccaattc actaaaaaat acttttttga taatgcactc tcgcccgagg tatcgctttt
      301 atacgaagtg ctgaatgaaa aaagtgcagg ccttcccgag atacaattcc cgtatggaaa
      361 agcgcgcgtg tatcaagaag actcgcaagg ctctgaagct tttttaggcg aagactgggc
      421 acaggaaacc ggagcgggac aaaaattatc tctcaaactg ggacaagcaa aagaagtgcg
      481 tgtgaagcgc agcgttgtta aacgcaaaga agaaatcgtc aatttgccga ttaaaaatta
      541 ccgcgagacg ataaaatttc agattgaaaa tttcaaagac agcgcggtgc ctctcacttt
      601 ggtcgaacac ccaggcggtg aatggattgt cgacggcatc gaagtaaaag aagaaagcgg
      661 cgagcgcgat accctgcgcg aaaacacggt gagcgttgat gtaaaagatg aacgtatcga
      721 cacgaataac ttgaggctgc atgtgaaggt acccgcgaac ggacacgaga ggaaaaaatt
      781 gaacgtgtac gtaaagctgg tacttaaaaa ccgttggtga ataattccta aataatattt
      841 tccgcgccgt cgcgacgatc gactttgccg gatgcggtcg tcgcgcgcca ctacctcttt
      901 catcttcgtt acacttctcc tcgacatcat tggcttcggt ttggtgattc cagtgatgcc
      961 aaaaatggtg gcacgcttca ccacgaacga cattgaaacc aataactggt atatggtgat
     1021 gaccgcaagt tacggcatca tgcagttttt tttctcgcca attttgggcg cgctctccga
     1081 taaatttggt cgccgcccaa ttttgctttt atcgattgtg ggtttgggct gtaattt
//
LOCUS       DAOMPD010000690         1133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44404_length_1133_cov_2.423006, whole genome shotgun sequence.
ACCESSION   DAOMPD010000690 DAOMPD010000000
VERSION     DAOMPD010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44404_length_1133_cov_2.423006"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLY93_15490"
     CDS             <1..486
                     /locus_tag="PLY93_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70926.1"
                     /translation="AASLECYSHYLNFEKETGSDPEAVSLAIDMAYRLEKFSLVRDYL
                     FSLTPKARENLATPTLILAIHSFIYLGQATEAEKIASFLRHRANAPQLPSFDAMVNEK
                     FGSTTKAKEFIQQHLNALPPKEFSADIQRSISIALAYMVENKHSEAEKVLLACKARHA
                     A"
     gene            complement(483..>1133)
                     /locus_tag="PLY93_15495"
     CDS             complement(483..>1133)
                     /locus_tag="PLY93_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70927.1"
                     /translation="SFAYIPWLKHHQRRLDEKALTEPSEKLKIYLGAREYFDSIGYDQ
                     IGMDHFGLKDDSLTKAFREKTLHRNFMGYTTQKTLDMLAFGTSAIGFVSGVYMQNHVK
                     LSGYRNAVETQSAWFEKGYAMSHEDRIRHEVITALMSNFSLNFENIEATFGIRFNEFF
                     SRELARLQEFSDDGHGHLTSSAFETTESGSFIIRHIASVFDAYRKQGEKKAFSKAI"
BASE COUNT          299 a          291 c          263 g          280 t
ORIGIN      
        1 gcagcctcgc tcgagtgcta ctcgcactac cttaacttcg agaaagaaac gggtagcgat
       61 cccgaagcgg tttctctcgc catcgacatg gcgtaccggc ttgaaaaatt ttcgttagtg
      121 agggactatc tttttagttt aacgccgaaa gcgcgtgaaa atttggccac gcccacactt
      181 atcctcgcca tccacagttt tatttattta ggtcaagcta ccgaggcgga aaaaattgcc
      241 agcttcctac gccaccgcgc caacgcaccg caactaccca gcttcgatgc gatggtgaat
      301 gaaaaattcg gctctacaac aaaagcgaaa gaatttatcc agcagcacct aaacgcgctt
      361 ccccccaaag agttctcggc ggatattcag cgctccatat caattgctct ggcgtatatg
      421 gttgagaaca aacacagtga agcggagaaa gtactgttgg cttgtaaagc gcggcacgcc
      481 gcttaaatcg ctttagaaaa cgccttcttc tccccttgtt ttcgatacgc atcgaatacc
      541 gacgcgatgt ggcgaatgat gaagctgcca ctctcggttg tttcaaacgc ggaagaagtg
      601 agatgaccgt gaccatcgtc ggaaaattct tgaaggcgtg ccaactcacg actaaaaaat
      661 tcgttaaagc ggatgccaaa cgttgcttca atattttcaa agttgagtga aaaatttgac
      721 atcagcgccg taatcacttc gtgacggatg cggtcttcgt gcgacatggc atagcctttc
      781 tcgaaccacg cgctttgggt ttcaaccgca ttgcggtagc ccgaaagttt gacgtggttt
      841 tgcatataga cccccgacac aaaaccaatc gcgctcgtgc caaaagcgag catgtcgaga
      901 gttttctgcg tcgtgtatcc catgaaattg cggtggagag ttttctcgcg gaacgcttta
      961 gtgagcgaat cgtctttaag cccaaagtga tccataccaa tttgatcgta tccaatcgag
     1021 tcaaagtatt cgcgagcacc caaataaatt tttaacttct cggacggctc tgtcaacgcc
     1081 ttttcatcca accggcgctg gtggtgcttg agccacggga tataggcgaa act
//
LOCUS       DAOMPD010000691         1132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44454_length_1132_cov_2.133705, whole genome shotgun sequence.
ACCESSION   DAOMPD010000691 DAOMPD010000000
VERSION     DAOMPD010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44454_length_1132_cov_2.133705"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLY93_15500"
     CDS             complement(<1..517)
                     /locus_tag="PLY93_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP70928.1"
                     /translation="MKEEDAQDEIHPGRIPPWCDIQKLTDIQGEAVEVVLESGTLSEA
                     TLNAICTQAFEKIGSALDHYQMREAQVQMAQQILRSFYNERSVVIEAATGVGKSFAYT
                     VAALAYNYLTGERVLLATETKALQWQLFEKDLPFIRSVLDTNLRFELALGSSNYFCRL
                     RYEDMLREGTHR"
     gene            complement(514..978)
                     /locus_tag="PLY93_15505"
     CDS             complement(514..978)
                     /locus_tag="PLY93_15505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRP70929.1"
                     /translation="MALHSNMSAERYILFCDGASRGNPGNASFGVACFKGSTSPTLSE
                     FKQNETSAVFAVGFPLGMATNNEAEYRAILYALEECKKRGISSPTLYSDSELVVKQLN
                     GLYKVKDAKMRSLFTQAMPLIRAIHPTIVHVRREKNQIADFLANRALDEALL"
BASE COUNT          251 a          292 c          299 g          290 t
ORIGIN      
        1 tgcggtgtgt gccttcgcgc aacatatctt cgtagcgcaa gcggcaaaaa taattagacg
       61 agcccaacgc gagctcgaag cgtaaattgg tgtcgagcac cgaacggatg aacggcaaat
      121 ctttttcaaa aagctgccac tgcaatgcct tggtttcggt ggcaagaagc acccgctcac
      181 ccgttaaata attgtacgcg agagcagcca ccgtgtacgc aaatgattta cccacgccgg
      241 tggccgcttc gatgaccacg ctgcgctcgt tgtaaaaaga gcgtaaaatt tgttgcgcca
      301 tttgtacttg cgcttcgcgc atttggtagt ggtcgagtgc gctaccaatt ttttcaaacg
      361 cttgcgtgca gatggcgttg agagttgcct ccgaaagcgt tccactttcc aacacgactt
      421 cgaccgcttc gccctggatg tcagtgagtt tttggatatc gcaccacggc ggtatgcgcc
      481 cggggtgtat ttcgtcttgt gcgtcttctt ctttcacagt aaagcctcat cgagcgcgcg
      541 gttggctaaa aaatcggcga tttggttttt ttcacggcgc acgtgcacaa ttgtgggatg
      601 gattgcgcgg atgagcggca ttgcctgtgt aaaaagagaa cgcattttcg cgtctttgac
      661 tttgtaaagg ccgttgagtt gtttcaccac cagctcgcta tccgagtaaa gcgtggggga
      721 tgaaataccg cgcttcttac attcctctaa tgcatagagg atcgcgcggt attcggcctc
      781 gttgttggtc gccatcccca agggaaatcc tactgcaaaa acagcgctcg tttcattttg
      841 tttaaattcg ctaagcgtgg gagaagtact ccctttgaaa cacgcaacac caaacgatgc
      901 attccccgga ttgccgcgcg atgcaccgtc gcagaaaaga atataccgct ctgcgctcat
      961 gttcgaatgc aacgccacat ccgtgcgtct tgagtttgca cggggatttt ttctttgcac
     1021 cgttcctcgg cgtacgagca gcgcggtcta aacgcacacc cttcgatggc ttcggttaaa
     1081 gcagggggag agccagggat ggcgaaaagt tttttcccgc gtgtgcgctc cg
//
LOCUS       DAOMPD010000692         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44546_length_1130_cov_3.276279, whole genome shotgun sequence.
ACCESSION   DAOMPD010000692 DAOMPD010000000
VERSION     DAOMPD010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44546_length_1130_cov_3.276279"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1095
                     /locus_tag="PLY93_15510"
     CDS             82..1095
                     /locus_tag="PLY93_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70930.1"
                     /translation="MVQIRNLWHLCTYSVTAWFVRRRSRQNAKSIRFENIARALVMVG
                     AITTSAYPAPNESKIYKVALPEVWTTNESSADAYESSFHAGFTRSRRVQLDTSNTSEM
                     LKAQFSKSVQKEETTQLTGDDCPSCLSDIAKAMSRNYDYVVSLSVKELKNATQISVQF
                     AVVRQGLPPIQGYTVQAYKTDSDVLELIEAIAEYISYSEFTLRAKVLHIDTEEEKVSL
                     DVGRNYKIEKGNVFIILSEAIQKQEEEDIDIDAYSIRELGRVKVTEVSGKSSTAELSK
                     GKLFNFKTEGGKIIAELEQTEKLEQSKKKYYELLKKNKEKIKIADSKDQNIADKKKEA
                     EEV"
BASE COUNT          389 a          222 c          252 g          267 t
ORIGIN      
        1 cattgatcca tagcatcagc gtttgcttgg atcgttcaat ttttttgcaa taaaaatcgc
       61 ccttgcgtac atgggaaagg tatggtacaa attagaaatt tatggcattt atgcacatac
      121 tcggtaacag catggttcgt gcgaaggcgc agtcgtcaaa atgcgaaatc cattcgtttt
      181 gagaacatcg cacgcgcgct cgttatggtc ggcgctataa ccacaagcgc atatccagca
      241 ccgaacgaat cgaagatata taaagttgct ctacccgagg tttggacgac caacgagtct
      301 tctgcagatg cctacgaaag tagctttcac gcgggtttta cccgttcaag gcgtgtgcaa
      361 ctcgacacca gcaataccag tgaaatgctg aaagcccaat ttagtaaaag cgtacagaaa
      421 gaagaaacaa cgcagctcac gggggacgat tgccccagtt gcttaagcga tattgcaaaa
      481 gcgatgagcc gaaattatga ttatgtggtt tcattgtcgg tgaaagagct taaaaatgca
      541 acacaaattt cagtgcagtt tgctgtggtg cgtcaaggac tgccaccgat tcaagggtac
      601 accgttcagg catataaaac cgactctgac gttttagagc tcattgaagc gattgcggag
      661 tacatttcat acagcgagtt cactctacgc gctaaagttt tgcatatcga tacagaagaa
      721 gaaaaagttt cactggatgt gggtcgcaac tataaaattg aaaagggtaa cgtcttcatt
      781 attctcagcg aggcgataca aaaacaagag gaagaggata tcgacatcga tgcatattct
      841 ataagagaac taggtcgcgt taaggtgaca gaggtttctg gaaagagtag caccgccgaa
      901 ttatccaaag gtaaattatt caattttaag acagaaggtg ggaaaattat cgcagaactc
      961 gagcaaaccg aaaagcttga acaatccaaa aaaaaatact atgagctatt aaagaaaaat
     1021 aaagaaaaaa ttaagatcgc cgacagcaaa gatcaaaata tcgccgacaa gaaaaaagaa
     1081 gcagaagaag tctaaaccct caagacatat gttacacttt ttataaagtg
//
LOCUS       DAOMPD010000693         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44556_length_1130_cov_2.038140, whole genome shotgun sequence.
ACCESSION   DAOMPD010000693 DAOMPD010000000
VERSION     DAOMPD010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44556_length_1130_cov_2.038140"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..349)
                     /gene="dgt"
                     /locus_tag="PLY93_15515"
                     /pseudo
     CDS             complement(1..349)
                     /gene="dgt"
                     /locus_tag="PLY93_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001971457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
     gene            555..>1130
                     /locus_tag="PLY93_15520"
     CDS             555..>1130
                     /locus_tag="PLY93_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70931.1"
                     /translation="MKSYMTSITGLVLLLGAVAFHIEAQIQTSVPVTPDNQAQLVNVA
                     GINTPYSEYTPFITADEHFLYFQSNRPGGVASSGDFDLWFSENMATNGTPEFKMPVNV
                     DLPVNSAYFDGHPSLRQLPSGEYEMYFCSFAIDDREGPEQTNIYYTIQKNGKWTIPAP
                     VIEVNTEFHDRMPSISQDGRYLYFSSDRPGGF"
BASE COUNT          269 a          304 c          263 g          294 t
ORIGIN      
        1 agttgaggcc gttgaattcg ggataacgct cttcgagctc ggtgacgatg cgtagcgtgt
       61 gttcgttgtg atcgaacccg ccgtactgtt tcattttttc gtgcaatgcc cgctcacccg
      121 catgcccaaa cggcggatga cccaagtcgt gcgcgagcgc caccgtttcg gttaaatctt
      181 cgttgagttt taacgctcgc gcaacggtgc gcgcaatttg cgccacctca atcgaatgcg
      241 ttaagcgcgt gcggaagtta tcccccaaat ggtttacaaa gacctgggtt ttatattcta
      301 aacggcggaa gtatttcgag tgtacaacgc ggtcgcggtc gcgttggaac tcggtgcgcg
      361 aaagcggcgg cgcctcggcg aacgcacgct tttcaaactc aatcccttgt gctgctaaaa
      421 aaatgtgcat acgttaattg gtgcggcaac gcgcggaagt ctcgactttt acggatgcca
      481 tacctgtctc tatttaccta tcgacaccaa tatatttgtt cttcaccgtg tatgtataac
      541 tatcaagttt acccatgaag tcttatatga ccagtataac tggccttgtg cttttattag
      601 gcgccgttgc tttccacatc gaagcccaaa tccaaacgag cgttcccgta acgcccgaca
      661 accaagcaca gcttgtcaac gttgcaggta tcaacacacc gtacagcgag tacaccccat
      721 ttattaccgc tgatgagcat tttctctatt ttcaatccaa ccgcccggga ggtgtcgcca
      781 gttcgggcga ttttgatctt tggttttctg aaaatatggc caccaacggc acacccgagt
      841 ttaagatgcc cgtgaatgtc gacctgccgg tgaactcggc atactttgac ggccacccgt
      901 cgttgcgaca attaccctcg ggggaatacg aaatgtattt ttgctcgttt gccatcgacg
      961 accgtgaggg cccagaacaa accaacattt attacactat tcaaaagaac ggcaagtgga
     1021 caatccccgc accagtcatc gaagtgaata ccgaattcca cgaccgtatg ccatcgattt
     1081 cacaagatgg ccgctatctt tattttagct ccgacagacc aggaggtttt
//
LOCUS       DAOMPD010000694         1128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44657_length_1128_cov_2.085741, whole genome shotgun sequence.
ACCESSION   DAOMPD010000694 DAOMPD010000000
VERSION     DAOMPD010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44657_length_1128_cov_2.085741"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..590
                     /gene="leuD"
                     /locus_tag="PLY93_15525"
     CDS             33..590
                     /gene="leuD"
                     /locus_tag="PLY93_15525"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRP70932.1"
                     /translation="MRNIDTDQIIPKNFLKRVERTGFGAFLFNDWRFNDDGSENKDFV
                     LNKPEHKGAHILLARDNFGCGSSREHAPWALLDYGFKVIIAPSFADIFYNNCFQNGIL
                     PIALESQKMDELFNQVKANAATEFSIDLEKKEITYNNKTIHFELREPMRQKLLQGLDD
                     IGWTLQFENEITQHEKKFVAGIPAV"
     gene            complement(587..>1128)
                     /locus_tag="PLY93_15530"
     CDS             complement(587..>1128)
                     /locus_tag="PLY93_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70933.1"
                     /translation="EKAKQLAAAQEKVQAKVELVKSDGISDKKFTLSLGLGVNHGLLL
                     VGEHLGLITTLNLFASMQMMRYLEPHIRFEGYYGLAKENVSNLVGGSILSGLGITAHF
                     GRFALMPYIDGGLFMSRIDAKAGIFNTLLPSVGAGFSITFMPTPTWGLALQTHAQYVL
                     DMDMPALFIGASLASVLRF"
BASE COUNT          334 a          279 c          234 g          281 t
ORIGIN      
        1 aaacaatcgt atcaaaagct tgccccctcg acatgcgcaa catcgatacc gaccaaatca
       61 tccccaagaa ttttttgaag cgcgttgagc gcaccggttt tggcgcattc ctctttaacg
      121 actggcgctt caacgacgac ggcagcgaga ataaagactt tgtgttgaat aaaccagaac
      181 acaaaggagc gcacatcctt ttggcgcgcg ataatttcgg ttgtggttct tcacgcgaac
      241 acgcgccttg ggcactttta gactatggtt tcaaagtcat catcgcgccc tcgtttgccg
      301 atattttcta caataattgt tttcaaaacg gtattttacc gattgctctc gaatctcaaa
      361 agatggatga actcttcaac caggtgaaag ccaatgcggc gacagagttc tctatcgatc
      421 ttgaaaaaaa ggaaattact tacaacaata agactatcca ctttgagcta agagagccga
      481 tgcgccaaaa acttttgcaa ggcttagacg acatcggttg gacgctgcaa tttgaaaacg
      541 aaatcactca gcacgaaaaa aaatttgtag cgggaatacc cgccgtttaa aagcgcagta
      601 ctgacgcgag cgatgcgccg ataaacaacg ccggcatatc catatccaac acgtattgcg
      661 catgcgtttg caacgcgagt ccccaagtgg gtgtcggcat aaatgtaatg ctaaacccag
      721 cacctaccga aggtaacagt gtattaaaaa tgcctgcttt tgcgtcgata cggctcataa
      781 ataatccacc gtcgatatac ggcatcaaag cgaagcggcc aaagtgagcg gtaatcccca
      841 gccccgacaa aatactgcca ccgacaagat tcgacacatt ctctttcgct aaaccataat
      901 agccttcaaa gcggatgtgt ggttccaaat aacgcatcat ttgcattgag gcaaaaagat
      961 tgagagtagt aatcagccct aagtgttctc ctactaaaag gagtccatgg tttacgccca
     1021 atcccaaaga caaagtaaat ttcttatcgc ttataccgtc gctcttaaca agttcgactt
     1081 tggcttgtac tttttcttgc gcagcggcaa gctgtttggc tttttcct
//
LOCUS       DAOMPD010000695         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44712_length_1127_cov_2.105410, whole genome shotgun sequence.
ACCESSION   DAOMPD010000695 DAOMPD010000000
VERSION     DAOMPD010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44712_length_1127_cov_2.105410"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLY93_15535"
     CDS             complement(<1..734)
                     /locus_tag="PLY93_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRP70934.1"
                     /translation="MKIKYYGVRGSLPVSGAGFDRFGGNTTCCYIQHEDTKIVIDAGT
                     GIRILGLDLMRTDLPQTGGHFSMLFTHTHWDHIQGFPFFIPAFLPNVTLDVYGETKEV
                     PGLDDTRETWNIERILKMQQHFIYFPVGTKYMSSNKKFHEIKAGSTFTIGKIKISTFA
                     LHHPNSTLAYRFDVDGKSFVFSTDVEHNDEMLVKLGEFARNPDVLAYDCQYTTAEYIA
                     GKIGWGHSTYESGIQICKNGDVKNLH"
     gene            764..>1127
                     /locus_tag="PLY93_15540"
     CDS             764..>1127
                     /locus_tag="PLY93_15540"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70935.1"
                     /translation="MRLVDNFFTPTHTNTNSLGRNRNTIVDVKLFTTRFNFPAIKDLG
                     ANEILGFGADLEPDTLLYAYTHGFFPWYNEPPITWQAPLVRMVLRKNEIHVSRSLKKF
                     LRRSTHTVRKDTAFREVIH"
BASE COUNT          307 a          260 c          231 g          329 t
ORIGIN      
        1 atgtgtagat ttttaacgtc gccgttttta caaatttgga tgccgctttc gtacgttgaa
       61 tgtccccacc caatttttcc agcgatgtac tcggcagtgg tgtattgaca atcgtacgcc
      121 aacacgtccg ggttcctcgc gaactctcct agcttcacca gcatctcatc gttgtgctcc
      181 acgtcggtgg aaaacacaaa cgatttaccg tccacatcga atcgatacgc gagtgtactg
      241 ttgggatggt ggagagcaaa tgtagatatt ttaattttac cgatggtgaa cgtacttcct
      301 gctttaattt cgtgaaattt tttattcgac gacatatatt ttgtacccac cggaaagtaa
      361 ataaagtgct gctgcatttt taagatacgc tcgatattcc atgtttctcg cgtatcgtcg
      421 agcccaggca cttctttcgt ttcgccatag acatcgagag tcacgttggg taaaaatgca
      481 ggtataaaaa aaggaaaacc ttggatgtga tcccaatgcg tgtgcgtaaa aagcatcgaa
      541 aagtgcccac ccgtttgggg taaatcggtg cgcatcaaat cgagacctaa aatacgtatt
      601 cctgtgccag cgtcgatgac gattttggtg tcttcatgtt gaatataaca acacgttgtg
      661 ttgccaccaa aacggtcaaa acccgctcca ctaactggca acgagccgcg tactccgtaa
      721 tatttaattt tcaatggcac ccttatttta tatcgtgttt ttaatgagac ttgtcgataa
      781 tttttttact ccaacacaca cgaatacaaa ttcattaggg cgcaatagaa acacgattgt
      841 tgatgtaaaa cttttcacaa cccgtttcaa ttttcctgca atcaaagatt taggcgcaaa
      901 tgaaattctg ggcttcggtg cagatttaga acccgatacg ttactttacg cctacacgca
      961 tggatttttt ccctggtaca atgaaccgcc gattacatgg caagcgccgc tcgtgcggat
     1021 ggttctcaga aaaaatgaaa tccatgtttc gcgttcgctt aaaaaatttt taagacgctc
     1081 gacgcatacc gtacgcaagg acaccgcgtt tcgtgaagtc atccacc
//
LOCUS       DAOMPD010000696         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44782_length_1125_cov_3.428037, whole genome shotgun sequence.
ACCESSION   DAOMPD010000696 DAOMPD010000000
VERSION     DAOMPD010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44782_length_1125_cov_3.428037"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLY93_15545"
     CDS             <1..375
                     /locus_tag="PLY93_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRP70936.1"
                     /translation="VSGAKAKRAEFRFPDSTANPYLCFASMMMAGLKGIQEKIHPGEP
                     MEEDLFELTLDEIREKGIQQMPHTLREAVETMLVHRAIFEKGDVFTKEFIDTYKELKF
                     ETEIWPWEARPHPFEFLSTYSC"
     gene            439..>1125
                     /locus_tag="PLY93_15550"
     CDS             439..>1125
                     /locus_tag="PLY93_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRP70937.1"
                     /translation="MDLATDSNATIGLSTQIVSAHRAKFGFNEIPEKRASNILRVAQK
                     FWNLSAWMLEAIALLSWFLGRTTDFIVALSLLIVNALISVVQERRAEGVVESLRARLR
                     VEARVLRDGVWQVLPARELVPGDVVRLRMGDFVPADSRILTGALLVDQSALTGESHAI
                     EATTDAVVYSGSMVRRGEATCRVNATGRRTYFGKTIELVDIAKPRLHIESIVARLVRW
                     LFLITGILALL"
BASE COUNT          258 a          283 c          311 g          273 t
ORIGIN      
        1 gtatcgggag ccaaagccaa gcgcgccgaa ttccgtttcc ccgattcaac ggcaaacccc
       61 tatctctgct tcgcgtcgat gatgatggct ggccttaaag gtatccaaga aaaaatccac
      121 ccaggcgaac cgatggaaga agatttgttc gagcttaccc tggacgagat tcgtgaaaaa
      181 ggaatccagc aaatgccgca cacgttacgt gaagcggtgg agacgatgct cgtccaccgt
      241 gcgatttttg agaagggcga tgtttttact aaagagttta tcgacactta caaagaactc
      301 aaatttgaga cagaaatttg gccatgggaa gcgcgccccc atccgtttga atttttatcg
      361 acgtacagtt gctagggttg cgagcctgtg cgccataaaa attttgtaga gagcctctta
      421 tagacacaag cgttagaaat ggatttagcc acagactcta acgctaccat cggtctctcc
      481 acacagattg tctccgcgca tcgcgcaaaa ttcggtttca atgaaattcc ggaaaaacgc
      541 gcgtcgaaca ttctgcgcgt ggcgcaaaaa ttttggaatc tttcggcgtg gatgctcgag
      601 gccatcgcgc tgctttcatg gtttttagga cgcaccaccg attttatcgt ggcgctctct
      661 ctcctcattg tcaacgcgtt gattagcgtt gtacaagaga ggcgcgccga aggcgtcgtt
      721 gaaagtttgc gggcacgact ccgcgttgag gcgcgcgtgc tgcgtgacgg cgtgtggcaa
      781 gtattgcccg cgcgcgaatt ggttccggga gatgtggtgc gtttacgcat gggagatttt
      841 gtgccggcgg attcacgcat tttaacaggc gctcttcttg tggatcagtc tgcgctcacc
      901 ggcgaatcgc atgcgataga agccacgacg gatgcagtgg tttattcggg ctcgatggtg
      961 cgccgtggcg aagcgacttg ccgcgtcaat gccaccggca ggcgcacgta tttcggcaag
     1021 accatcgaac tggtggatat cgcaaagcca cgcttacaca tcgaaagcat tgtcgcacgg
     1081 ttggtacggt ggctgttctt gatcaccggt atcctcgcgc tgctt
//
LOCUS       DAOMPD010000697         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_44799_length_1125_cov_2.108411, whole genome shotgun sequence.
ACCESSION   DAOMPD010000697 DAOMPD010000000
VERSION     DAOMPD010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44799_length_1125_cov_2.108411"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /gene="queD"
                     /locus_tag="PLY93_15555"
     CDS             <1..391
                     /gene="queD"
                     /locus_tag="PLY93_15555"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRP70938.1"
                     /translation="IGLNIWSSSKGMIIEKEFVFDAAHFLPYVHEKHKCRRLHGHTYT
                     VTIRVEGDLNAIGWVVDFADITKVVQPILHQLDHTLLNAVHGLENPTAEIIAMWIYER
                     VKENIKGVRRVTVQEGFAARAIYPHNA"
     gene            400..1116
                     /gene="queC"
                     /locus_tag="PLY93_15560"
     CDS             400..1116
                     /gene="queC"
                     /locus_tag="PLY93_15560"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRP70939.1"
                     /translation="MEKPIAIVLLSGGLDSATCLALALEQGCIPIALSFDYGQRHKNE
                     LIASKEITAHFSVSHVILPVGLDTIGGSALTANVDVPKGGKDLLNENKIPVTYVPVRN
                     LIFLSLAYALAEVRNAQEIFIGVNSIDYSGYPDCRPDFIAAFAHAATLASKVGRDGNA
                     PKIITPLAALSKSDIAQEAVRLKVPVQSTWSCYDPQIRNGKYYPCENCDACILRRNGF
                     TTARLALSKEEIFGSPQELP"
BASE COUNT          299 a          257 c          266 g          303 t
ORIGIN      
        1 aattggatta aacatttggt cttcctcaaa aggaatgatt attgaaaagg aatttgtttt
       61 tgatgcagca cattttttac cctacgtcca cgagaaacac aaatgccgcc gtctccatgg
      121 tcatacgtac acggtgacga ttcgcgttga gggcgatctc aacgcaattg gatgggttgt
      181 ggatttcgcc gacattacaa aggtggtgca acccatttta caccaactcg accacacact
      241 cttgaacgcc gtccatgggt tggaaaatcc aactgccgaa attattgcga tgtggattta
      301 cgaacgggtc aaagaaaata ttaaaggggt gcgccgtgtc accgtacaag aaggctttgc
      361 agcgcgcgcc atttatcctc acaacgcata aaataagcta tggaaaaacc aattgctatt
      421 gtacttttat cgggcgggct tgattcggca acctgcttgg cgctcgcgct ggaacaaggg
      481 tgtatcccca ttgcgctttc gtttgattac ggccaacggc acaaaaacga actgattgcc
      541 tcaaaagaaa ttactgcaca ttttagtgtt tctcatgtga tcttaccggt tgggctcgac
      601 accattggcg gttcggcttt gactgcgaat gtggatgtcc ccaaaggggg caaagatttg
      661 ttgaatgaaa ataaaattcc tgtgacgtat gttccggtgc gcaatcttat ttttttatcg
      721 ttggcatacg cgctcgccga ggtgcgtaac gcacaagaaa tttttattgg cgttaattca
      781 atcgattatt ccggctatcc cgattgcaga ccggatttta tcgcggcgtt tgcacatgcg
      841 gccacgctgg cgtcaaaagt ggggcgtgat gggaatgctc cgaaaatcat aacaccgctg
      901 gctgcgctct ccaaaagcga tattgcccaa gaagcagtcc gcctcaaagt tcctgtacaa
      961 tcgacctggt cttgctatga cccacaaatt agaaatggta aatactatcc atgtgaaaat
     1021 tgcgacgctt gcatcttgcg tcgcaacggt tttacaacag ctcggttagc gttatctaaa
     1081 gaagaaattt ttggttctcc acaagagttg ccataaagcg gctgg
//
LOCUS       DAOMPD010000698         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_45147_length_1118_cov_2.542803, whole genome shotgun sequence.
ACCESSION   DAOMPD010000698 DAOMPD010000000
VERSION     DAOMPD010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45147_length_1118_cov_2.542803"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>1118
                     /locus_tag="PLY93_15565"
     CDS             274..>1118
                     /locus_tag="PLY93_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70940.1"
                     /translation="MSQTIRFSANKTWMNWQSLARAHELDMLMHTGFKKWPAAFSTDY
                     MKVDELAPIEKWAAELRQIDAQSLIVIGIGGSSLGGIALKALTDPHSDRLVFWEGPHP
                     IHMQHIAQVVENRQVALLFISKSGTTLESRANLALFRQFFPGVPEYFVSSEPEKIQDL
                     GSTAQNTFLIPKNLGGRFSIVSPVGMMPALFVDYNVKIFLTSFAEANAEWEITIPVAE
                     NVAKQTAIHFYNLFAARFTGVAFWVYASELVSWGHWLVQLWAESLGKKPHITALPMVV
                     KGPED"
BASE COUNT          288 a          264 c          265 g          301 t
ORIGIN      
        1 gggtgagtgt gtcgtatctt atttatgacc tcattcaatt ttgggaaaaa gcgtcaaaaa
       61 aatgtaccaa attcgtcgtt gggggtaact atgtgcccaa agttttggag tggtcgaaaa
      121 gaagagtaaa ttttgaggaa aagctctctt tcaggcgggc gggaccccac tttttcacct
      181 gtcgaacccc ctaattattt cgcagcggtt atttaagaac atgtatccat gcaataattc
      241 tttacaacca aaaccgcgcg ttgaaataca cacatgtctc aaacaatccg cttctctgcc
      301 aacaaaactt ggatgaattg gcaatcgctc gcgcgcgcac atgaactcga tatgctcatg
      361 cacacgggtt tcaaaaaatg gccggcggca ttttctaccg attatatgaa agtcgacgaa
      421 cttgctccca tcgaaaagtg ggcggcagag ctgagacaaa ttgacgcgca gagtctcatc
      481 gtcatcggca ttggtggttc gtcgttggga ggcattgcgc tcaaagcgct tactgatccc
      541 cactccgatc gtctcgtttt ttgggaaggt cctcatccga tacacatgca gcacatcgcc
      601 caagttgtgg aaaataggca agtcgcactt ttatttattt ctaaatcggg aacgactctc
      661 gaatcgcgcg ccaatcttgc gctcttccgt cagtttttcc ccggcgtacc cgaatatttt
      721 gtgagcagcg aaccggaaaa aatccaagat ttgggttcca cagcgcagaa tactttttta
      781 attccaaaaa atttaggagg ccgctttagt attgtttctc ctgtgggtat gatgcctgca
      841 cttttcgttg attacaacgt gaaaatattt ttaactagtt ttgccgaagc caacgccgag
      901 tgggaaatca ccattcccgt cgccgaaaac gtggcgaaac aaacggcaat acacttttac
      961 aatttattcg ccgcacgctt taccggtgtc gcgttttggg tgtacgcatc tgagctcgtc
     1021 agttgggggc attggttggt gcagctttgg gcagaatcgt tgggtaaaaa gccacacatc
     1081 accgcgctgc cgatggtcgt caaggggccg gaggatca
//
LOCUS       DAOMPD010000699         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_45412_length_1113_cov_2.320416, whole genome shotgun sequence.
ACCESSION   DAOMPD010000699 DAOMPD010000000
VERSION     DAOMPD010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45412_length_1113_cov_2.320416"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLY93_15570"
     CDS             complement(<1..775)
                     /locus_tag="PLY93_15570"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRP70941.1"
                     /translation="MYHQKNYLPFESDASDRPWLFPFIRLRGDTPGPKITLIAAIHGD
                     ELNGIRMLHRLESLFIEKEIPLKGSICLVPVANLMGYTNHSRYLPDRRDLNCIFPGLR
                     EGSEGARLAYFIWSHFVSDADFVIDVHSGSYNRWNFPHVRGDMKNEKMRAYAAQLTGH
                     PILHSAGVVGSLRKEASKLGKPVFLVEAGETGRFEKRVVEEGLTLLLKILAVLGIVNP
                     ADVQEFLPNAISTHPHGYYRKAVWQRATHAGLFIPQVEPG"
BASE COUNT          292 a          288 c          259 g          274 t
ORIGIN      
        1 cgcccggctc aacttgagga ataaaaagcc ccgcatgcgt cgcgcgttgc cacaccgctt
       61 tacggtaata cccatgcggg tgtgtgctga ttgcattcgg tagaaactct tgcacgtccg
      121 cagggttgac aatgcctaac acagctaaaa ttttaaggag cagtgttaat ccctcttcga
      181 cgacacgttt ttcaaaacgg ccagtctcac ccgcctcgac caaaaaaaca ggtttgccca
      241 atttgctcgc ttctttacgc agactaccca caacacctgc gctatgaagg atgggatgcc
      301 ccgtgagctg cgctgcgtac gcccgcattt tttcgttttt catatcgcca cgcacatgcg
      361 gaaaattcca acggttgtaa ctccctgaat gcacgtcgat gacaaagtcg gcgtcggaga
      421 caaaatgcga ccaaatgaaa tacgccaaac gcgccccctc gctcccttcc cttaggcccg
      481 ggaagataca gttcaagtct cgcctgtcgg ggaggtagcg cgaatggttg gtgtacccca
      541 tgagattggc aaccggaacc aaacagatgg aaccctttag tgggatttct ttctctataa
      601 aaagcgactc gagtcggtgc agcatacgga tgccattcaa ctcgtcgcca tgaatagcgg
      661 caatcaatgt aattttgggt ccgggcgtat cgccacgcag gcgtataaaa ggaaaaagcc
      721 acggcctatc cgacgcgtct gactcaaaag gaaggtaatt tttttgatgg tacacttaaa
      781 ttaaggtaac tatctaatgc acagtgaatt tgacgattat tttctctgta aaacccccaa
      841 tacgaaaccc atatttgagt tgaccggctt ggtggggtat agacgtatta acttatatgt
      901 agacgatgcc gttccacgac aacgtctatt tttttacacg gaggaatatg aaaaaaatta
      961 aactcacact cgcctttttg gcaatggggc tttttacagc acccgctttc tcgatatttc
     1021 aaataggggt acagggcaat taccagtcgc tctcgtacga accgtcttcg ggcggtacgt
     1081 caagcaagta ctcaggcacc ggccttggcg gtt
//
LOCUS       DAOMPD010000700         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_45728_length_1107_cov_2.470532, whole genome shotgun sequence.
ACCESSION   DAOMPD010000700 DAOMPD010000000
VERSION     DAOMPD010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45728_length_1107_cov_2.470532"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLY93_15575"
     CDS             complement(<1..535)
                     /locus_tag="PLY93_15575"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRP70942.1"
                     /translation="MKVSFLAFLVFAGSLFAVEGRVTYLTGKATIKSGKKSQALKLGG
                     TIREGDVISTAKDSRLGVQMSDGGSFLINPMTTLEVRPKSTYYQAQGAISMNFRKGGK
                     DATSKWAIKTPVVTAGVRGTSFTVDASKKSTRVVMFQGKVIMTDFVRETGLQSDANLM
                     MQDFLNDIELHAGTALRY"
     gene            complement(588..>1107)
                     /locus_tag="PLY93_15580"
     CDS             complement(588..>1107)
                     /locus_tag="PLY93_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70943.1"
                     /translation="AVNVDQYKMPLEVRATDSGVASSVDTSASAFNLALDAAYHQMLQ
                     DGKLRVIYSAGVGSTNMTYLNKASASESSFESSHFYIPLGVAVEHQTFETFKTRIGVR
                     KNIFSNKSLKDATPTQTTTQATSFYLDDELLLATGFGWVPAEKVQIDFALNATVLSST
                     MLFSSVSARYHY"
BASE COUNT          271 a          271 c          268 g          297 t
ORIGIN      
        1 cgtaacgcaa cgcggtaccg gcgtggagtt caatgtcgtt caaaaaatct tgcatcatga
       61 ggttggcatc gctttgtaag cccgtctcgc gcacaaagtc ggtcatgatg actttgcctt
      121 ggaacatgac aacgcgagtc gattttttac ttgcatcgac ggtaaaagag gtgccgcgta
      181 cgcccgccgt gactacgggg gttttaattg cccacttact ggtagcatct ttaccgcctt
      241 tacgaaagtt catgctaatt gcaccctgcg cttgataata cgttgattta gggcgtactt
      301 ccagcgttgt cattgggttg attaaaaaag atccgccgtc ggacatctgc acgcctagtc
      361 tgctgtcttt agcggttgaa atcacgtcgc cttcgcgtat ggtgccgccg agcttgagtg
      421 cttggctttt cttgccgctt ttgattgtgg ccttgccggt taaataagtc actcgacctt
      481 caacggcaaa gagactaccc gcaaacacga gaaatgctaa aaacgatact ttcataaagg
      541 ctccttttat acttattaga cgcataccac ccttaaaaag gtcgttttta atagtggtag
      601 cgcgccgaaa ccgagctaaa aagcatggtt gaacttaaaa cagtggcgtt caacgcaaag
      661 tcaatttgca ctttttccgc cggtacccag ccaaagccgg ttgccaacag gagctcgtcg
      721 tccagataga aagaagtcgc ttgtgttgtg gtttgagtcg gtgttgcatc tttaagcgat
      781 ttattggaga aaatgttctt acgcacaccg atacgcgttt tgaatgtttc aaacgtttgg
      841 tgctcaaccg caacgcctaa ggggatataa aagtgcgaac tttcaaagct actttcagaa
      901 gcactcgctt tattcaaata cgtcatgttg gtcgatccga cacccgccga gtaaatgacg
      961 cgcagtttac cgtcttgcag catttggtgg tacgctgcat cgagtgccaa gttaaacgcc
     1021 gacgccgagg tatcgactga agacgccaca cccgagtcgg tcgcgcgcac ttcgagcggc
     1081 attttatatt ggtcgacgtt cacagcc
//
LOCUS       DAOMPD010000701         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_45830_length_1105_cov_3.809524, whole genome shotgun sequence.
ACCESSION   DAOMPD010000701 DAOMPD010000000
VERSION     DAOMPD010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45830_length_1105_cov_3.809524"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="PLY93_15585"
     CDS             complement(<1..>1105)
                     /locus_tag="PLY93_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRP70944.1"
                     /translation="EEKIEWHNPVYHWLEARYKTLIEFLIARYKKIVYVAFSIVTVLI
                     IFGGIKIGSEFLPELDEGNLTLRGYFPVGGSLQAANKSVPKILDLLAKNKMIANAVAQ
                     MGRNDSGTDPLPPNRLEITMALHDYKKWDEKITKENLLIRIIKDLEHEFPAIRWSFSQ
                     PIMDNMSEAIMGTIADMAVFVYGPDLKIARQKAIEILEIVKTIPGASEYGLEQEADSP
                     QLTIQIKRDVAARYGINVNTIQSLIEKGVGAQRVSTLYESSRRFGIVVRFPYEYRKSV
                     KALRALPVFAPTGERIALSTLADIDIIDGPTMIFRQEGKRAITVRSNIRGRDQGSFVA
                     ELQKKVNAQVKLPEGYSLQYGGNYENLARVGKQL"
BASE COUNT          278 a          259 c          246 g          322 t
ORIGIN      
        1 gagctgtttt cctacgcgtg cgaggttctc gtagtttccc ccgtattgga gcgaataccc
       61 ctctggcagt ttaacctgtg catttacttt tttttgtagc tcggcgacaa aagatccttg
      121 gtcgcggcca cggatatttg agcggacggt aattgcgcgt tttccctctt ggcgaaaaat
      181 catcgtgggg ccatcaataa tgtcgatgtc ggcaagggtt gaaagcgcaa tcctttctcc
      241 tgtcggagca aaaacaggta aagcgcgtaa cgctttgacg cttttacggt actcgtacgg
      301 aaaacgtaca acaatcccaa aacggcgtga gctttcgtaa agggtcgata cccgctgtgc
      361 cccaacgcct ttttcgataa ggctttgtat tgtattgacg ttgatgccat accgcgcagc
      421 gacatcgcgt ttaatttgga ttgtcaactg tggcgaatcg gcttcttgtt ctaaaccata
      481 ttccgacgca cccgggattg ttttcacaat ttctaaaatt tcaatggctt tttgtcgtgc
      541 gattttcaaa tcggggccgt acacaaaaac cgccatgtcg gcaatcgtcc ccatgattgc
      601 ctccgacata ttatccataa tgggctggga aaaactccaa cggattgcag gaaactcatg
      661 ctcaagatct ttaataatac gaatgagtaa attttccttg gttatttttt catcccattt
      721 tttataatcg tgcaacgcca tggtaatttc aagacggttg ggcggcaagg ggtcggtgcc
      781 cgaatcgtta cgccccattt gtgctacggc attggctatc atcttgtttt tggcgagcaa
      841 gtctaaaatt ttaggcaccg atttattcgc cgcttgaagc gagccgccca cgggaaaata
      901 accgcgcagt gtcaagtttc cttcgtcgag ctctggcaaa aactcagaac caattttaat
      961 cccaccaaaa ataataagaa ctgtgacgat actaaatgca acgtagacta tttttttgta
     1021 acgtgcaatc aaaaactcaa tgagtgtttt atagcgcgct tccagccaat gataaacagg
     1081 gttgtgccat tcgatttttt cttcg
//
LOCUS       DAOMPD010000702         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_45966_length_1103_cov_2.355916, whole genome shotgun sequence.
ACCESSION   DAOMPD010000702 DAOMPD010000000
VERSION     DAOMPD010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45966_length_1103_cov_2.355916"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1103
                     /locus_tag="PLY93_15590"
     CDS             <1..>1103
                     /locus_tag="PLY93_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRP70945.1"
                     /translation="KIMADIFKDAPDRSAPVTLNGKTETVSNGSVVIAAITSCTNTSN
                     PSVLLAAGLVAQKAAKAGLKIPATVKTSLAPGSRVVTKYLEAAGLQKALDAIHFNTVG
                     YGCTTCIGNSGPLDPALSEVINKNNLAVSAVLSGNRNFEGRIHADVKANYLMSPPLVV
                     AYALAGTLDFDFENEKIQGKVYLKDLWPSQKEVTDALASAVTADLFKKEYAHVFTGNP
                     MWNDIKVSEGKTYKWDTNSTYIAKPDYFENFSLQEPGIPVLKDMRCLAVFGDSVTTDH
                     ISPAGNIKKDSPAGKYLLARGVEQKDFNTYGARRGNHEVMVRGTFANTRIKNLMTAPD
                     AVSGNSVDWTKVPEGGNSVHIPSGEKMSIFDTA"
BASE COUNT          313 a          283 c          267 g          240 t
ORIGIN      
        1 ataaaatcat ggccgatatt tttaaggacg cccccgaccg cagcgcacca gtgacgttaa
       61 acggcaagac agaaactgtc agcaatggtt cggttgtaat tgcggcgatc acctcgtgca
      121 caaatacgtc aaatccatcg gtactcttgg ccgcaggtct tgtcgcacaa aaggcagcca
      181 aagcgggtct taaaatccct gcaaccgtaa agacatcact cgcaccgggt tcgcgcgtgg
      241 taactaaata cctcgaagcg gcgggcttac aaaaagcgct tgacgcaatc catttcaaca
      301 ccgtgggtta tggttgcaca acatgtatcg gtaactctgg accactcgat cctgcacttt
      361 cagaggtcat caataaaaac aatttagcgg tgagcgcagt gctttcgggt aaccgtaact
      421 ttgaaggccg catccacgcc gatgtcaaag cgaactactt gatgtcaccg cctttggttg
      481 tggcgtatgc gctcgcaggc actctcgatt ttgattttga aaatgaaaag atccaaggta
      541 aagtatatct caaagacttg tggccttcgc aaaaggaagt taccgatgct ctcgcatccg
      601 cagtgaccgc cgacttattc aaaaaggaat acgcgcacgt tttcaccggg aaccccatgt
      661 ggaatgatat caaagtgagc gaaggcaaaa cgtataagtg ggataccaac tcgacctaca
      721 ttgccaaacc cgattatttc gagaatttct cgttgcaaga accgggcatc cccgtgctaa
      781 aagacatgcg ttgcttggcg gtctttggtg actcggtcac taccgaccac atttcaccag
      841 cggggaatat caaaaaagac agccctgcgg gtaaatactt gctggcgcgt ggcgtcgaac
      901 aaaaagattt caatacgtat ggcgcacgcc gaggcaacca cgaagtgatg gtgcgcggta
      961 catttgcaaa cacccgcatt aaaaatttga tgaccgcacc cgatgccgta agcggcaaca
     1021 gcgtcgattg gacaaaagtt cccgagggcg gcaattcagt acacattcct tcgggcgaaa
     1081 aaatgagcat cttcgacaca gcg
//
LOCUS       DAOMPD010000703         1102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46029_length_1102_cov_2.145177, whole genome shotgun sequence.
ACCESSION   DAOMPD010000703 DAOMPD010000000
VERSION     DAOMPD010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46029_length_1102_cov_2.145177"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLY93_15595"
     CDS             complement(<1..954)
                     /locus_tag="PLY93_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70946.1"
                     /translation="MNDASERNLDTPMMRQYLEIKKQYPDDILFFRMGDFYEMFLEDA
                     VYASRVLDIALTRRQDKVPMCGVPHHSMSQYVHPILEQGRRIAICDQLENPAEAQGRI
                     VRRGVTRILTPGSLFEESLIDAKDSRRLCGMSVGKEYIHLAIVEVATGQIWFERLSLR
                     LRTEGGVDSETVNKIVERNRNDFIHIAAAFRIVEWVVSDKDTAQIADESTLNVAQIRR
                     YPIDDVAFQERVLKESLQSHNLAVWELDAAEKRALTLTLAYLREISPLLKLNWASPVR
                     EYRHKIMTLDEATLRTLEILQSTDGKTKPSLIGLFTPLATVA"
BASE COUNT          260 a          291 c          268 g          283 t
ORIGIN      
        1 tgccaccgtc gcaagcggtg taaaaaggcc aatgaggctc ggctttgttt taccgtcggt
       61 actttgtaaa atttccagtg tacgtagcgt cgcttcatct aacgtcataa ttttgtgacg
      121 gtactctcgc acaggcgatg cccagttaag tttcaaaagc ggcgaaattt cacgtaagta
      181 cgcgagcgtg agcgtaagcg cacgtttctc ggcggcatcg agttcccaca cggcgaggtt
      241 gtggctttgt agagattctt ttaatacacg ctcctgaaaa gcaacatcat ctatggggta
      301 gcggcgtatc tgcgcaacat tcaaggtaga ttcatcggca atctgcgcag tatctttatc
      361 gctcacgacc cactcgacaa tgcgaaatgc agcagcgatg tgaataaaat catttcggtt
      421 gcgctcaacg attttattca cagtttcgct gtcgacgcct ccctctgtgc gcagtcgaag
      481 ggagagacgc tcaaaccaaa tttggccggt ggcgacttcg acaatcgcca gatggatgta
      541 ctctttaccc acgctcatac cgcaaagccg ccgtgaatct tttgcgtcga tgaggctctc
      601 ttcaaaaagc gaacccgggg tgaggatgcg cgtcactcca cggcggacaa tacgcccttg
      661 cgcttcggca gggttttcca actgatcgca gatggcgatc cgccgccctt gctctaaaat
      721 gggatggaca tactggctca tcgaatggtg cggcacgcca cacatcggca ctttatcttg
      781 ccgtcttgtg agcgcgatgt cgagtactcg cgacgcgtac accgcatctt ctaaaaacat
      841 ttcatagaaa tcgcccatgc gaaagaagag aatatcgtcg gggtactgtt tttttatttc
      901 gagatactgc cgcatcatcg gcgtgtctaa attcctttcg gaggcatcgt tcacgcgcca
      961 cctcctggaa atctttccac gacggtcgct gcgcgactgt cttgaaaaga tgtagtatgt
     1021 tgtgcaattt ttataaactg tgaaactttg tcatgactct ttatgcccga cgacgactca
     1081 acaccgctcg cgacatctac ac
//
LOCUS       DAOMPD010000704         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46183_length_1099_cov_2.174330, whole genome shotgun sequence.
ACCESSION   DAOMPD010000704 DAOMPD010000000
VERSION     DAOMPD010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46183_length_1099_cov_2.174330"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLY93_15600"
     CDS             complement(<1..215)
                     /locus_tag="PLY93_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004502099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRP70947.1"
                     /translation="MSKKIGVLGSGGVAQTLAAGFVKHGYAVKMGTRDAGKLADFAKK
                     NTAIAIGSFEEAAAFGEIIVLAVKGKVV"
     gene            complement(228..587)
                     /locus_tag="PLY93_15605"
     CDS             complement(228..587)
                     /locus_tag="PLY93_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRP70948.1"
                     /translation="MAESAFATTGLSPAHAFILMSVNRQPGIQPTELAAAMQLERSTV
                     TRFVDSLEAKHYLKRESAGRRMHLYPEKSARALDKKLRAAWSSLFERYASALGIDNAR
                     KLTRETYSAYKKIKIDK"
     assembly_gap    943..952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          274 a          309 c          246 g          260 t
ORIGIN      
        1 accaccttgc ctttgactgc cagcacgata atttcaccga aggcagcggc ttcttcgaat
       61 gaaccaatcg cgatcgccgt attcttcttt gcaaaatccg ccaatttacc cgcatcgcgc
      121 gtccccattt ttaccgcgta gccatgtttc acaaaaccag ccgccagcgt ctgtgcaacg
      181 cctccagacc ctaggactcc aatttttttt gacattttca aatcccccta tttatcaatt
      241 tttatttttt tgtaagccga ataggtctcg cgcgtcagtt tacgcgcatt gtctatcccg
      301 agcgcgctgg cgtatcgttc aaacaaggaa gaccacgctg cgcgcagctt cttatccaag
      361 gctctcgcac tcttttctgg ataaaggtgc atgcgacgcc ccgcggattc cctctttaga
      421 taatgctttg cttccagcga atcgacgaaa cgggtcactg tggaacgctc aagctgcatc
      481 gcagcggcga gctcagtcgg ctgtataccc ggctgtcggt taaccgacat aagaataaat
      541 gcgtgcgccg gcgataaccc ggtcgtcgca aatgcgctct cagccatgcg cgtcatagac
      601 ctacccagcg cattagcggt aaagtagagg caattgcaga aaggcgaatc attttctccc
      661 gacaccggtc agtctttata atttagttgt acatacaatt atataaatat ttcagaacgc
      721 acccctggat gcgggagcgt cgacgtagag tcactgttta tctcgcaggc gagaactttc
      781 attgcgcgac ttactcacga aagacagaga cctgtgcaat cgcagcgcga tttctcggtc
      841 tcccggaccc cgaagaacca tgcaaatcgc catagtgatt tgccaactaa acgcattcgc
      901 gatgtagttc actgcgtgaa cctgacaggt cggcgaagcc gannnnnnnn nntaacccaa
      961 gcgttttgac ccgtcaaagc gccatgggtg ttcgggatgg gaacggatgc aaaccttgct
     1021 cgaatgcaca taaatgacct acaaaactaa aaaggctccc gaaggagccc tataaagcat
     1081 gacagcccaa acctgtgcg
//
LOCUS       DAOMPD010000705         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46324_length_1096_cov_3.181556, whole genome shotgun sequence.
ACCESSION   DAOMPD010000705 DAOMPD010000000
VERSION     DAOMPD010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46324_length_1096_cov_3.181556"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..992)
                     /locus_tag="PLY93_15610"
     CDS             complement(36..992)
                     /locus_tag="PLY93_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRP70949.1"
                     /translation="MLPNRTTTFVRIEGRYFFFLFWAASLLSLFSIVRTHKNIDTFPY
                     HADYEIHNSDPGIDEGLWREKAAQYIFLVVVDNLTESDIAAMPRFTELRKKAALFSLK
                     APFPSLSLPAYTTLLTGAPPTITGISSDDNETIDARIDSLPRRLKAAGWKTAVVGSTV
                     WLNYFENDFDLGAVDSFYNRKSKFFKERNSTNAGESKGRSVLWHGVYQPTPDGWRRFI
                     QRYNIAYNFGDDVATAEVEDTSRTRKAIALWKKEGVDFVLLHLQTPPHSALAELDLNF
                     QRVADAVKLQTSILAVVPAHGAKKMLWGTYHGGDEKTVRRVP"
BASE COUNT          292 a          262 c          266 g          276 t
ORIGIN      
        1 tagacaccag gttttattgc aggccccatg agagtctaag gaacgcgccg tactgtcttt
       61 tcatcgccgc catggtacgt gccccaaagc attttttttg caccgtgcgc aggcacaacc
      121 gccaaaatac tcgtttgtag ttttaccgca tcggcgacgc gctggaaatt taagtcgagc
      181 tcggctaatg ccgagtgtgg cggcgtttgc aaatgcagca acacaaaatc cacgccttct
      241 tttttccata acgcgatggc cttgcgagtg cgcgacgtat cttctacttc ggcggtggcc
      301 acgtcgtcgc caaagttgta cgcgatgttg taacgttgaa tgaatcgccg ccaaccatcc
      361 ggagtgggtt gatacacacc atgccagagc acgctgcggc ctttgctctc tcccgcgtta
      421 gtggaatttc tttccttaaa gaattttgat tttctattgt aaaacgaatc gacggcgcct
      481 aaatcaaaat cgttctcaaa gtaattaagc cacaccgtcg aaccaacaac ggcggttttc
      541 caaccggccg ctttaagacg gcgtggcagc gaatcgatgc gtgcgtcgat agtttcgtta
      601 tcgtccgagc ttatgcccgt aatggtaggc ggcgcgccgg tgaggagtgt tgtgtaagca
      661 ggtaaagaca acgatgggaa aggagctttt aaagaaaaga gcgccgcctt ttttcgtagt
      721 tcggtaaagc gtggcatcgc tgctatatcg ctctccgtga gattatccac tacaactaaa
      781 aaaatatatt gtgccgcctt ttcacgccat agcccttcgt cgattcccgg atctgaatta
      841 tggatttcat aatcggcgtg ataagggaac gtatcgatgt ttttatgcgt gcgtacaatg
      901 ctgaataaag agagtaaaga tgccgcccaa aaaagaaaaa agaaatatcg gccttctatt
      961 ctaacaaacg ttgtcgtgcg gtttggtagc acaaagacac actacgtaca gcgacacggg
     1021 gaaaaccaaa aaacaagaaa atatgcaacc cacagagata actgacactt ttggggtcaa
     1081 gttgagtgcg ttttaa
//
LOCUS       DAOMPD010000706         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46483_length_1093_cov_2.419075, whole genome shotgun sequence.
ACCESSION   DAOMPD010000706 DAOMPD010000000
VERSION     DAOMPD010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46483_length_1093_cov_2.419075"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLY93_15615"
     CDS             <1..270
                     /locus_tag="PLY93_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70950.1"
                     /translation="IMLGSTDAKPAGAAATPILAAKPLSVTKPTRTTSFLVSGHEPVK
                     NIKVAPKQTSVAEATKSESNATGGFEYEMAGSEDELDKQFEEIRP"
     gene            267..>1093
                     /locus_tag="PLY93_15620"
     CDS             267..>1093
                     /locus_tag="PLY93_15620"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRP70951.1"
                     /translation="MSALGLFSQIPELTDVVWQRLSAFIEKELGIRMPIGKKSFLQNR
                     LWRRIKHYGFKTFDEYCDFLFSDKGKVMERTSFFNEITTNKTFFFREYRHFEFLSDRI
                     LPEYVANFTHAHTRLRVWSAACSTGEEIYSIACIIEEYMRAENVVIPYEIVGSDISTR
                     VLWHAQCGVYEEEQIRMISPDILRRYFYAGKGDKAHLRRVKPEIRSRIKLRKINLMND
                     AYPHTNQFDVIFCRNALIYFSPEDISQIVTRATHYLKPGGYFIIGHADAIYDASTKLR
                     "
BASE COUNT          295 a          261 c          269 g          268 t
ORIGIN      
        1 attatgttgg ggagcacaga cgcgaaacct gccggtgcgg cggcaacccc catactcgcc
       61 gcgaaacccc tgagcgtgac gaaacccact cgaaccacgt cgtttttagt gtcggggcac
      121 gagccggtga aaaacataaa agtagcgccg aagcaaacga gcgtggccga agccacaaaa
      181 agtgaatcaa atgccaccgg aggctttgag tatgaaatgg cgggctcgga ggatgaactc
      241 gataaacagt ttgaagaaat aagaccatga gcgcgttagg gcttttctcg caaatacccg
      301 aactcaccga tgtggtgtgg caacgcttga gtgcttttat tgaaaaagaa cttggcattc
      361 gcatgcccat cggcaaaaaa agttttttgc agaatcgctt atggcgccgg atcaaacact
      421 atggctttaa aacttttgac gaatactgcg attttttgtt tagcgacaaa ggtaaagtga
      481 tggagcgcac gtcatttttt aacgagatca ccacaaataa aactttcttt tttcgtgagt
      541 accggcactt tgagtttttg agcgaccgga ttttacccga gtacgtcgcg aactttaccc
      601 acgcgcatac gcggttacgt gtttggagcg cggcgtgctc gaccggcgag gaaatttatt
      661 cgatcgcgtg cattatcgaa gaatacatgc gcgctgaaaa cgtcgtgatt ccttatgaaa
      721 tcgtgggatc ggatatttca acgcgggtgc tgtggcacgc gcagtgcggc gtgtacgaag
      781 aagaacaaat ccgtatgatt tcccctgata ttctgcgccg ctatttttac gccggcaaag
      841 gcgataaagc gcacttgcgg cgtgtcaaac cggaaattcg ttcgcgtata aaattgcgca
      901 aaattaattt gatgaacgac gcatacccac acaccaacca gttcgatgtt attttttgcc
      961 gtaatgcgct catctatttt tctccagagg atatttctca aattgtgacg cgcgccacac
     1021 actacctaaa gcctggcggt tattttatta tcggccacgc cgacgccatt tacgacgcct
     1081 caacaaaatt gcg
//
LOCUS       DAOMPD010000707         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46607_length_1091_cov_2.144788, whole genome shotgun sequence.
ACCESSION   DAOMPD010000707 DAOMPD010000000
VERSION     DAOMPD010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46607_length_1091_cov_2.144788"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLY93_15625"
     CDS             complement(<1..633)
                     /locus_tag="PLY93_15625"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRP70952.1"
                     /translation="MKNPRNNNGISPKKNAGVLHGKTLLIAAGGTGGHISPGVSVAEA
                     WLAESGSVLFATLQKNIDYPDIVRLARNEKAAIVAYDTPRLTKNPLQILNFFKRFFAA
                     YRLLKRAARAERVDAVLGMGGYSSFPAVCFALFNRKPLFLCEQNARWGVVTRLGRFFA
                     RNIFLSFETQKPLSAKYRVTGNPLRAMFKGLVLSPIKKTRPQKKPLLFFLG"
     gene            complement(630..>1091)
                     /locus_tag="PLY93_15630"
     CDS             complement(630..>1091)
                     /locus_tag="PLY93_15630"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70953.1"
                     /translation="MVVPIMKRLAVPFAVGQKVKFLVRNHMFHYTDEWTDKAVRRFVK
                     KIPLEELQDLIALRLADRKGSGKKSAFPRALQNLIQHIDEIIAQEKKLKIKDLAIDGH
                     TLMELGMNPSRAMGDMLKYLFEEVKAERLPNERNLLIEAVKARLATTSTNV"
BASE COUNT          254 a          265 c          279 g          293 t
ORIGIN      
        1 ccctaagaaa aaaaggaggg gctttttttg cggtcttgtt ttcttgattg gcgagagcac
       61 aagcccctta aacatcgcac gcagcggatt accggtgacg cggtattttg cgctcaaggg
      121 cttttgtgtt tcaaaggaaa gaaatatatt acgcgcaaaa aaacgcccca gccgtgtcac
      181 tactccccaa cgtgcatttt gttcgcagag aaaaagtggc ttgcggttga agagcgcaaa
      241 acacaccgca gggaaagaac tatatccacc catgccaagc acagcatcga cgcgctcggc
      301 gcgcgccgcg cgttttaaga gtctatacgc cgcaaagaat cgtttaaaaa agtttaaaat
      361 ttggagtgga tttttagtca ggcgcggcgt gtcgtacgcg acgatagcgg ccttttcgtt
      421 cctcgctaaa cgcacaatgt cggggtaatc tatatttttt tgtagcgttg cgaaaagaac
      481 gctgccgctc tcggcgagcc acgcctcggc aacgctgacc ccggggctaa tgtgaccgcc
      541 ggtgccgcct gcggcgatga gcaatgtttt accgtggagt acaccggcat tttttttagg
      601 ggagattcca ttgttgtttc ttgggttttt catacgtttg tggatgttgt ggcgagacgc
      661 gctttgaccg cctcaatgag aagattgcgt tcgttgggca agcgttcggc cttgacctct
      721 tcaaaaagat acttgagcat atcgcccatt gcccgcgagg gattcatccc caattccatg
      781 agagtgtggc cgtcgatggc gaggtcttta attttcaatt tcttttcttg ggcgatgatt
      841 tcatcgatgt gttgaatgag attttgtagc gcgcgcggga atgcactttt tttccccgag
      901 cccttgcggt ctgcaaggcg tagcgcaatc aaatcttgca actcttccag aggaattttt
      961 tttacaaaac gccgcaccgc cttgtctgtc cattcgtcgg tataatggaa catgtggtta
     1021 cgcactaaaa attttacttt ttgaccaacc gcaaacggca cagccaggcg cttcatgatg
     1081 ggaaccacca t
//
LOCUS       DAOMPD010000708         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46632_length_1091_cov_1.374517, whole genome shotgun sequence.
ACCESSION   DAOMPD010000708 DAOMPD010000000
VERSION     DAOMPD010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46632_length_1091_cov_1.374517"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLY93_15635"
     CDS             complement(<1..236)
                     /locus_tag="PLY93_15635"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRP70954.1"
                     /translation="MSFVERIRSVFRFNHPLWAGAFRPLFFLSAVFGMSTLLLWPFMY
                     FKGLGSPGQVNTMSLVESHGYQMLVGFILAALAG"
     gene            complement(233..985)
                     /locus_tag="PLY93_15640"
     CDS             complement(233..985)
                     /locus_tag="PLY93_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRP70955.1"
                     /translation="MQSLPIKLDVRNVSHRFARQNILESISFTLHTGKSTVFLGPSGV
                     GKTTLFRICADLIEAEKGKLTRYYTNLGYMFQSPRLLPWQTAFENMALALKARHTRSH
                     EIRERVIHTAKMLGLTADDLTKYPYQLSGGMQSRVAMARALIIQPDVLSLDEPFTGLD
                     IGLKSELMQVLKAELQKGTAILLITHELADAVELADQILLLSGEPGKIIESITIDTPV
                     HTRDPAVLNHELQRLMTNPKVAEAFALKKGFL"
BASE COUNT          276 a          255 c          283 g          277 t
ORIGIN      
        1 aaacccgcca atgcagcgag gataaacccc acaagcattt ggtaaccgtg cgattcaacc
       61 aagctcatcg tattgacttg gccggggctt cccaagcctt tgaagtacat aaacggccac
      121 aggaggagag tcgacatccc aaaaactgcc gagagaaaaa agagaggacg gaaggcgcct
      181 gcccataaag gatggttgaa ccggaaaaca ctgcggatgc gttcgacaaa actcataaaa
      241 atcctttttt gagcgcgaac gcttcggcga ctttagggtt cgtcatcagg cgttgcaact
      301 cgtggttcaa aaccgccgga tcgcgcgtat gcaccggtgt gtcgatcgta atgctctcga
      361 taatttttcc aggctcgccg cttaacagta aaatttggtc ggcgagttcg actgcatcgg
      421 caagttcgtg cgtgatcaaa agaatcgccg tccctttttg tagttcggct ttgagcactt
      481 gcatgagttc actttttaag ccgatgtcga gtccggtaaa aggttcgtcc aaagataaaa
      541 catcgggctg aatgatgagc gcacgcgcca tcgcgacacg gctttgcatg ccccccgaaa
      601 gttggtaagg gtacttggtt aagtcgtctg cggtgaggcc gagcatcttt gcagtatgga
      661 taacacgttc gcggatttca tgggagcggg tgtggcgtgc tttgagcgcc aacgccatat
      721 tttcaaacgc ggtttgccat ggcagtaacc ggggggattg aaacatatac cccaaattgg
      781 tgtagtagcg tgtgagcttc cctttttcgg cttcgatgag gtcggcacaa attctaaaaa
      841 gtgttgtttt tcccaccccc gatggtccca aaaacacggt ggatttaccg gtgtgcagcg
      901 taaagctaat cgattctaga atattttgcc gtgcaaaccg gtgcgataca tttctcacgt
      961 cgagcttaat cggtaaactt tgcatagagt tattaattat taaaatgccc tttgcgtgtt
     1021 ggtgtcgcgc catctttctg tatagcgttt aatgcgttct aaaatcaagt gttcaacggc
     1081 gagcagaaga c
//
LOCUS       DAOMPD010000709         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46746_length_1088_cov_2.411423, whole genome shotgun sequence.
ACCESSION   DAOMPD010000709 DAOMPD010000000
VERSION     DAOMPD010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46746_length_1088_cov_2.411423"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLY93_15645"
     CDS             complement(<1..591)
                     /locus_tag="PLY93_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRP70956.1"
                     /translation="MLIRLVLFFSIIPTIPVFAYRVVIDPGHGGRDLIPHTLYGDKFD
                     RRQGVYLDKFREGARARGVWEFEVMYDIATKVKVLLDKTTTSEGKRDFKKILQKYGRL
                     KANEVNPIEAYISREGGYIENYFENREDPNARFRLYDYPNYKTGEIEPGTISRINALT
                     PELVITLHLTHTEAPVQGGMATVITPGYQTYKTALAY"
     gene            complement(912..1073)
                     /locus_tag="PLY93_15650"
     CDS             complement(912..1073)
                     /locus_tag="PLY93_15650"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70957.1"
                     /translation="MKFEARAKLKKIRPRTIGQASRISGVDPSDIDILLITLENLRRK
                     KETIQRAIV"
BASE COUNT          256 a          225 c          275 g          332 t
ORIGIN      
        1 atacgcgagc gcagttttat acgtttgata accaggggta atcaccgttg ccatgcctcc
       61 ctgcactggc gcctcggtgt gggttaagtg cagcgtgatg acaagctcgg gggtgagcgc
      121 gttgatgcgg ctaatagtgc ccggttcaat ttctcctgtc ttgtaattgg ggtaatcgta
      181 aaggcgaaaa cgcgcattgg gatcttcacg gttttcaaaa taattttcga tatacccacc
      241 ttcgcgcgaa atgtacgcct caatgggatt tacctcattt gcttttaggc gtccatattt
      301 ttgcagaatt tttttgaagt cgcgtttccc ttcggaggtt gtggttttgt cgaggagtac
      361 tttcactttc gtagcaatat catacatcac ctcgaactcc cacacgccac gcgctcgcgc
      421 gccttcgcgg aatttatcga gatacacgcc ttgtcgtcgg tcgaatttat cgccgtagag
      481 agtatgcggt attaggtcgc gcccgccatg gcctgggtcg atcaccacgc ggtacgcaaa
      541 aacgggtatt gtggggataa tagaaaagaa aaggacaagt cgaatgagca cgggtaaaag
      601 ttgttttgaa ggactcggtg acaaatatct tcagtatgta agaattgtaa ctatttgtac
      661 gtatgggggt ttgttgtact cagacaaact tcgggtcctg tattttggtt tatattttga
      721 agtcttggtc gtagaattat gtcacattag gggataacta taccgatttt ttgagattat
      781 gtcgcattag ggtataacta taccgatttt ttaaaattat gtcacattgg gaggtaacta
      841 tgcaccgcga gtgctggatt actcagtatt ttgaacttac tatagtgtct aatagacacg
      901 ccagagtctt gttagacaat cgcgcgttgt atagtctctt tcttccgccg caaattttct
      961 aacgtgatga gtaaaatgtc gatgtccgat gggtcgacac cgctgatgcg cgacgcttgg
     1021 ccgatggtgc gcggacggat ttttttaagt ttagcgcgcg cctcaaactt gatgctgtct
     1081 aagttgtc
//
LOCUS       DAOMPD010000710         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46752_length_1088_cov_2.287512, whole genome shotgun sequence.
ACCESSION   DAOMPD010000710 DAOMPD010000000
VERSION     DAOMPD010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46752_length_1088_cov_2.287512"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1088)
                     /locus_tag="PLY93_15655"
     CDS             complement(71..>1088)
                     /locus_tag="PLY93_15655"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRP70958.1"
                     /translation="SAALEQRELILQYKGDGIDWDFLLQQFSVTDWLHFIERLGFQTY
                     KGTSRRYFIREMKAGRLLKVWLSHLKNLGCEFFYRHTCTAFAQTHESIALQFSNGHIQ
                     SFSAVIFALGGASALPTPAAWPQLFEAHGVHIVPFAAANVGYNIAWKKDFIAEAANTP
                     LKNIEFISPLGRKKGDIMITEHGIEGTPVYFYGTTGACHLDLKIDLTAKEIEAKLSAV
                     AENLSPLRRAQKILRLNKATQALLFHEAPREALNSISAISQVLKKFPLRLENPRPLEE
                     AISSTGGIALDAIDSMFMLKKTQGVFVVGEMLDWSAPTGGFLIQACVAQGHAAAQGVI
                     NLKK"
BASE COUNT          274 a          247 c          272 g          295 t
ORIGIN      
        1 tatgtcacat cacgattttc ctaaaaatct gtccgacctc cccgccgagg gtaaggctta
       61 gattcttgga ttattttttt agattgatga ccccctgtgc ggcagcgtgt ccctgcgcaa
      121 cgcacgcttg aattaaaaag ccacctgtcg gtgcgctcca gtcgagcatc tcgccgacga
      181 caaaaacacc ctgtgttttt tttaacataa acatggaatc gatggcgtcg agagcgatgc
      241 cgcccgttga tgaaatggct tcttccaatg gacggggatt ttctaatcgt aaaggaaatt
      301 tcttgagtac ttgcgaaatc gcactaatgc tgtttaatgc ttctcggggg gcttcatgaa
      361 acaagagagc ttgcgtggct ttgttgaggc gtaatatttt ttgcgcacgg cgtaacggcg
      421 ataaattttc agcgacggcg ctcaacttcg cttcgatttc ttttgcggtt aaatcaattt
      481 ttaagtcgag atgacaagcg cccgtcgtgc cataaaaata aacgggggta ccttcgatgc
      541 cgtgttcggt gatcatgatg tcgccttttt tgcgtccaag cggcgagatg aattcaatat
      601 ttttgagcgg tgtgtttgcg gcttcggcaa taaaatcctt tttccatgcg atattgtaac
      661 cgacgttcgc ggcggcaaag ggaacgatat gaacgccgtg cgcttcaaaa agctgtggcc
      721 aagccgcagg cgtgggtagc gcacttgcgc cacccaaagc aaaaatgacc gcgctgaaag
      781 actgtatgtg tccgttagaa aattggagtg caatggattc gtgtgtttgt gcaaaagcag
      841 tgcatgtgtg acggtagaaa aattcacacc ctaaattttt caaatgcgaa agccaaacct
      901 tcaataagcg ccccgctttc atttcacgga taaaatagcg gcggcttgtg cctttatacg
      961 tttgaaagcc caagcgttcg ataaagtgca accaatcggt aacggaaaat tgttgcagta
     1021 gaaaatccca gtcgatgccg tcgcctttgt attggagtat caactcgcgt tgttcgagcg
     1081 cagcgctg
//
LOCUS       DAOMPD010000711         1085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_46872_length_1085_cov_2.618447, whole genome shotgun sequence.
ACCESSION   DAOMPD010000711 DAOMPD010000000
VERSION     DAOMPD010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46872_length_1085_cov_2.618447"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLY93_15660"
     CDS             <1..602
                     /locus_tag="PLY93_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRP70959.1"
                     /translation="LANLLSHGAGLGLAVAGTAFLIAHAVQSGDPFRVVSYSIFGAAM
                     VFLYSASSLYHSLWHPKIKKILRRMDHSAIFVAIAGTYTPILLVTLRGPLGWSFFGII
                     WGLALIGISFKIIFGHRYEAVSLTAYIVMGWLVVFMVKPVYLALSGPGLWLLLSGGLS
                     YTVGTIFYAMPRLPYHHMIWHLFVLGGSVCHYLCIYLYV"
     gene            complement(668..>1085)
                     /locus_tag="PLY93_15665"
     CDS             complement(668..>1085)
                     /locus_tag="PLY93_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70960.1"
                     /translation="FHNVRRFFFDATKISDGAHSLATREVHNSGGFHRILVDAPCSAS
                     GTLRRNPDVRFRILPHDIEKFAMTQLQILTNVHPYLKVGGKLIYTTCSVFREENESVV
                     EKFLSAHTDFKRLEQKVVGSPHLDADTMFYAVLEKK"
BASE COUNT          239 a          245 c          280 g          321 t
ORIGIN      
        1 agctcgcaaa tcttttaagt catggcgcgg gcttggggct tgcggtggct ggtacggcat
       61 ttctcatcgc gcatgcagtg cagagcggcg acccattccg agtggttagt tatagtatct
      121 ttggcgcggc gatggtattt ctttattctg cgtcgagcct ttaccattcg ttatggcatc
      181 ctaaaataaa aaaaattctg cgccgtatgg atcacagcgc catctttgtt gccatcgctg
      241 gcacttacac gccgattctc cttgtcacgc tgcgagggcc gttagggtgg agcttcttcg
      301 gaataatatg gggactggca ttgataggga tttcattcaa aattattttt ggccaccgct
      361 acgaagccgt ctcgctcact gcttatatcg tgatgggatg gcttgtagtc tttatggtaa
      421 aaccagtgta tctcgctctc tcggggcctg ggctatggct gcttctcagc ggtgggctct
      481 cgtacactgt gggcactatt ttttatgcga tgccacgatt gccttaccac cacatgattt
      541 ggcatttgtt tgtcttgggc ggtagtgtat gccactactt gtgcatttat ttatacgtat
      601 aagaaatatt atttggcttt agcgtacaaa aattaaaaaa atgtctcagc gagttgacgc
      661 acaggcttta ttttttttcc agtactgcat aaaacatcgt gtcggcgtcg aggtgtggcg
      721 accccacaac cttttgttcc agtcgtttga aatcggtatg tgctgataaa aatttttcaa
      781 cgacgctttc gttctcttcg cggaacacgc tgcacgttgt ataaatgagt ttgccaccta
      841 ctttcaggta gggatggaca ttcgttaaaa tctgtagttg agtcatcgcg aatttttcga
      901 tgtcgtgcgg taggatacga aagcgtacgt cggggttgcg gcgcagtgtg cccgacgccg
      961 aacacggcgc atcgaccaaa atgcggtgga atccgcccga gttgtggacc tcgcgcgtag
     1021 caagggagtg cgcaccgtca ctaattttag tcgcatcgaa aaagaaacgg cgcacattat
     1081 gaaat
//
LOCUS       DAOMPD010000712         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_47102_length_1081_cov_3.128655, whole genome shotgun sequence.
ACCESSION   DAOMPD010000712 DAOMPD010000000
VERSION     DAOMPD010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47102_length_1081_cov_3.128655"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLY93_15670"
     CDS             <1..186
                     /locus_tag="PLY93_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70961.1"
                     /translation="EPKQIICETLSRALAYPVYALFDSFFESPAKIFTASASIVSGLN
                     AAFAADGISGPNILSEN"
     gene            244..642
                     /locus_tag="PLY93_15675"
     CDS             244..642
                     /locus_tag="PLY93_15675"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70962.1"
                     /translation="MRWYSQSLSIKKYPGCAYIGGPVEACRKIFGVMQERRLHSGGIE
                     NVEVECNMLSKKMNDLAKPYLKGAQSSHTTLNFSLPVNCAAMLIDGGIVPATFTEDSL
                     VRKELWALANCVRVSHDIEATRITMQYRRC"
     gene            639..1070
                     /locus_tag="PLY93_15680"
     CDS             639..1070
                     /locus_tag="PLY93_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70963.1"
                     /translation="MKDSSTLVLKNLPRLTPYAHPMGGFFDAFALPFEWGLSFFKKNY
                     YPTENLVRPDEDFSRYHFLNPATVTVKLHSGEKLSASSMGPPGFAGTPEIEMEEMVFA
                     KLASTGIGSAAQKRICDFALHLRRLDADAMKNFVEWFMCQL"
BASE COUNT          276 a          245 c          256 g          304 t
ORIGIN      
        1 gaaccaaaac aaataatttg tgaaacgctt tcacgcgcac ttgcgtatcc cgtatatgcg
       61 cttttcgaca gtttttttga gtcgccagca aaaattttta ccgcatctgc ctcgattgtc
      121 tcggggctta acgccgcatt cgctgccgac ggaatttcag gtcccaacat tcttagcgag
      181 aattaagatt tctacaccac gttcgcgtac gcgccacttt ttgaggtttg gaacgacctt
      241 ggagtgcgtt ggtattccca atcgctttcg ataaaaaaat atccggggtg tgcgtatatc
      301 ggggggccag ttgaagcatg ccgtaaaatt tttggagtaa tgcaagaacg ccgtctccat
      361 tcgggtggta ttgagaatgt cgaagtcgag tgcaatatgc tttcgaagaa aatgaacgat
      421 cttgcaaaac cttatctcaa gggggcacaa agttcccata ctactcttaa tttttctctg
      481 cccgtaaact gtgcggcgat gctcatcgat ggcggtattg tccctgcaac attcactgag
      541 gattctctcg tacgcaaaga gctttgggcg cttgccaatt gcgtacgtgt ctcgcacgat
      601 atcgaagcga cgcgcatcac aatgcagtat cgtcgttgtt gaaggattct tctactttgg
      661 tgcttaaaaa tttgccgcgc cttacccctt atgcgcaccc catgggtgga ttttttgacg
      721 cattcgcatt accgtttgaa tggggactta gcttttttaa aaaaaattat tacccgacag
      781 aaaatttggt gcgccccgac gaagatttta gccgttacca ttttttaaac cctgcgacag
      841 taacagtaaa actccatagc ggtgaaaagt tgagcgcgtc gagtatgggt ccaccgggtt
      901 ttgccggaac gcccgaaatt gaaatggagg aaatggtgtt cgctaaatta gcttcgacag
      961 gaattggcag tgcggcgcag aaaagaatct gcgactttgc actacactta agaagactcg
     1021 atgcggacgc tatgaaaaat tttgttgagt ggtttatgtg ccagctataa ccactccgta
     1081 t
//
LOCUS       DAOMPD010000713         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_47121_length_1081_cov_2.013645, whole genome shotgun sequence.
ACCESSION   DAOMPD010000713 DAOMPD010000000
VERSION     DAOMPD010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47121_length_1081_cov_2.013645"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLY93_15685"
     CDS             <1..563
                     /locus_tag="PLY93_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRP70964.1"
                     /translation="MIENPIPTRAEVTDVANAAYEEVDSVMLSGETAAGKFPIKAVET
                     LDKILKRIEASGGIGWSREFKSDTVKGAFAGSAVELADELKAEAIAVFTRRGLTADFV
                     TAYHPQYPTVFAFTNMSQVRRKLLLNRACYPFRIDFSNDPERTIKTAFALLLKKKLLA
                     PGSRIVIVSDILAGSDRIEAIQVRTL"
     gene            570..>1081
                     /locus_tag="PLY93_15690"
     CDS             570..>1081
                     /locus_tag="PLY93_15690"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRP70965.1"
                     /translation="MKIYTKTGDKGETSLFGGGRVKKDHPHVEAYGTVDELNSHVGLL
                     IAHLRTLDFTKEPNFKENILPFLKVMQNRLFDLGAELAAGNEKFREKIQRRVAESDIT
                     PMEEAIDLMQEKLTLLKHFILPTGTIAASQAHICRTVARRAERAMVAMGDAPPVLIAF
                     INRVSDYFFVL"
BASE COUNT          272 a          289 c          267 g          253 t
ORIGIN      
        1 cgatgattga aaacccaata cccactcgcg ccgaggttac agacgtcgcc aacgccgctt
       61 acgaagaagt cgactcggtg atgctctcgg gtgaaactgc ggcaggcaag tttccgataa
      121 aggcggttga aacgctcgat aaaattctaa aacgtatcga agcgtcgggc gggattggtt
      181 ggtcacgcga gtttaagtct gatactgtta aaggcgcgtt tgcgggctcg gccgtcgaac
      241 tcgccgacga acttaaagcc gaagcgattg ccgtctttac acgccgcggc ctcaccgccg
      301 atttcgtgac ggcgtaccac ccgcaatacc ctacagtttt tgctttcaca aatatgtcgc
      361 aagtgcgccg caagctactc ctcaaccgcg cgtgctatcc tttccgtatc gatttttcca
      421 acgacccaga acgcacgatt aaaactgcgt ttgcgcttct actcaagaag aaactgttgg
      481 cacccggcag tcgcatcgtt atcgtgtcgg acattctggc gggttccgac cgcatcgaag
      541 cgattcaagt gcgcacactg tgaggcgtta tgaaaattta tacaaaaacg ggcgataaag
      601 gcgaaacttc cctcttcggt ggtggccgtg tcaaaaaaga ccatccgcat gtcgaggcgt
      661 acggcactgt cgatgaactc aatagccacg tgggactact cattgcgcat ttgcgtactc
      721 tcgactttac taaagagccg aacttcaaag aaaacatctt accgtttttg aaagtgatgc
      781 aaaaccgact tttcgattta ggcgcagagc ttgcggcggg gaacgaaaag tttagagaaa
      841 aaatccaacg ccgcgtcgca gaaagcgaca tcactcccat ggaagaagcg attgatttaa
      901 tgcaagaaaa attaactcta ctcaagcatt ttattttgcc caccggcact atcgctgcat
      961 cgcaggcgca tatatgccgc accgtcgccc gccgcgccga acgtgcgatg gtcgcgatgg
     1021 gtgatgcgcc gcccgttctc attgcgttta taaaccgcgt ttccgattat ttttttgtgc
     1081 t
//
LOCUS       DAOMPD010000714         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_47515_length_1074_cov_1.942100, whole genome shotgun sequence.
ACCESSION   DAOMPD010000714 DAOMPD010000000
VERSION     DAOMPD010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47515_length_1074_cov_1.942100"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLY93_15695"
     CDS             complement(<1..727)
                     /locus_tag="PLY93_15695"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRP70966.1"
                     /translation="MEVLFTIGGGILFAVWHSFCDALAWRIYHAYYSPERKKVENKVS
                     FIFYSSSRCEACRAHVPWYGLIPIAGFFLVQRKCRTCTRPLSLRFVVFETLAFLYGFF
                     LLHAQVPPLEFVVILVAYLCVWIVMTIDYRVLLIPTEAIFTLLVLGFIYLVVIRHPIW
                     MVLKNGFAPDIDLMLDLAVAFLWYAIFHLLRILSGYKMGAADVPLVLALGFLLGHPWA
                     IFLPGLAAFLAIGFYLLRRRSILI"
     gene            complement(728..>1074)
                     /locus_tag="PLY93_15700"
     CDS             complement(728..>1074)
                     /locus_tag="PLY93_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020773424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70967.1"
                     /translation="KEANTVQEIRGVLELKTPDQEDTPFLTLTYDFTKIPHAYRLAKD
                     NGIFEYAYYKYKPMLVKAHQFIKRKQITRALNYYRVIREQQIPSEFRHMVDRNIKDIT
                     EYLQKYLVTRAN"
BASE COUNT          346 a          242 c          237 g          249 t
ORIGIN      
        1 aaatgagtat cgagcgacga cgcagtaaat aaaagccaat cgctaaaaat gccgcaagtc
       61 ctggtaaaaa aatcgcccat ggatgcccca aaagaaatcc caacgcgagc accagcggta
      121 cgtcggctgc gcccatttta tacccagata aaatccgcaa gagatgaaat atcgcatacc
      181 acaagaaagc aaccgcgagg tcgagcatga gatctatatc tggcgcaaaa ccattcttga
      241 gaaccatcca aatcgggtgg cggataacta ccagataaat aaaacctaaa accaggagtg
      301 taaaaattgc ttcggtgggt atgagcaaca ctcggtaatc gatagtcatg acaatccata
      361 cgcataaata agcgacaaga ataacgacaa attcaagagg aggcacttgt gcgtggagca
      421 aaaaaaatcc gtataaaaaa gcaagcgttt caaacacaac aaaccgcaac gaaagaggtc
      481 gagtgcaggt gcggcatttt ctttgcacca aaaaaaaacc cgcaatcggt atcaatccat
      541 accaaggcac atgtgcccta cacgcctcgc agcgcgagga cgaataaaaa atgaaactta
      601 ctttattttc gacttttttg cgttcgggag aatagtacgc gtgataaatg cgccatgcca
      661 aagcgtcgca aaacgaatgc cacaccgcga agagaatacc gccgccaatc gtgaacagaa
      721 cttccattta gttcgcgcgc gtcaccagat atttttgtag atactcggtg atgtctttaa
      781 tgttgcggtc gaccatgtgt cgaaactcac tggggatttg ttgctcgcgg ataacacggt
      841 aatagttcaa cgcgcgcgta atttgcttgc gtttaataaa ctggtgcgct ttgacgagca
      901 tcggtttata tttatagtac gcgtattcaa aaataccgtt gtcttttgct aaacggtacg
      961 catgcggtat tttagtaaaa tcgtaagtca gcgttaaaaa cggcgtgtct tcttgatcag
     1021 gagttttaag ctcaagcacc cctcgaattt cttgaacggt gttggcttct ttat
//
LOCUS       DAOMPD010000715         1073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_47550_length_1073_cov_2.504912, whole genome shotgun sequence.
ACCESSION   DAOMPD010000715 DAOMPD010000000
VERSION     DAOMPD010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47550_length_1073_cov_2.504912"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLY93_15705"
     CDS             complement(<1..264)
                     /locus_tag="PLY93_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70968.1"
                     /translation="MLNNKIEVRGSDIEGHGLFAKAKICKGEFIWEKDADEKYFTQAE
                     IDALSPTEQKIFYNYAYQVGPNQFYGTLDGRAGAPQPRRALHQR"
     gene            complement(275..946)
                     /locus_tag="PLY93_15710"
     CDS             complement(275..946)
                     /locus_tag="PLY93_15710"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRP70969.1"
                     /translation="MTTIHHSSVETPEADEPPQLITPEVKCKISSHHAATTHGLEIRK
                     TKFGKGVFAKKEYRCGEIILRFKGRLYSKQAYLSKVKPEKCYYMQIDDEIFLGPTSSA
                     DNFVNHCCEPNAGLKKDKLKCFLVATQTIHIGQEITFDYSTSMAEDHWEMDCACGAPS
                     CRGRIRDFKYLPRALQYKYIELGIAPDFVMRSAGLFDPHDLFHANPVNPTNEMELVPF
                     HIAPA"
BASE COUNT          250 a          230 c          261 g          332 t
ORIGIN      
        1 tcgctggtgt agtgcgcgcc gaggttgcgg cgcgcctgcg cgtccatcga gcgtgccgta
       61 aaattggttg gggccgactt gatacgcgta gttatagaat attttttgct cggtggggga
      121 aagtgcgtcg atttcagctt gggtaaaata tttttcatcg gcatcttttt cccatataaa
      181 ttcgcctttg caaatttttg cttttgcaaa gagtccatgc ccttcgatgt cgctcccgcg
      241 tacttcaatt ttgttattta acatagacgc agtgttatgc aggggcgata tgaaaaggga
      301 caagctccat ttcatttgtg ggatttaccg ggtttgcatg gaatagatcg tgtggatcga
      361 aaagacccgc agagcgcatc acaaaatctg gcgcaatccc aagctcaatg tatttatatt
      421 gtaacgcacg gggtaaatat ttgaagtcac ggatacgacc acggcacgag ggagctccgc
      481 aagcgcagtc catttcccag tggtcttcag ccatgctcgt cgagtagtcg aatgtaattt
      541 cttggccaat gtgtatcgtt tgggttgcga caagaaagca cttaagttta tcctttttga
      601 gacctgcgtt gggttcacag caatgattca caaaattgtc ggcgctactg gttggaccga
      661 gaaatatttc atcgtcgatt tgcatgtagt agcatttctc tggtttcact ttgctgagat
      721 acgcctgttt agaataaagc ctacctttga agcgcagaat aatttcaccg catcggtatt
      781 cttttttggc aaagacgcct ttcccaaact ttgtttttct aatttctaag ccgtgcgtcg
      841 tcgctgcgtg gtgagagctt attttacatt taacctcggg agtgattaac tgtggaggct
      901 cgtcggcttc tggtgtttct accgacgaat ggtgtatcgt tgtcatggtc tcctcgacgc
      961 gtatcgcgtc gagtcgccct tcataaatct atcaatactg caagcataaa aatataattt
     1021 ttatgcaacc cacagagata actgacactt ttggggtcaa gttgagtgcg ttt
//
LOCUS       DAOMPD010000716         1067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_47943_length_1067_cov_1.906126, whole genome shotgun sequence.
ACCESSION   DAOMPD010000716 DAOMPD010000000
VERSION     DAOMPD010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47943_length_1067_cov_1.906126"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1067)
                     /gene="serC"
                     /locus_tag="PLY93_15715"
     CDS             complement(124..>1067)
                     /gene="serC"
                     /locus_tag="PLY93_15715"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRP70970.1"
                     /translation="VDGIKTTLGVPDEYAVLFIQGGASLQFSMVPMNLNVPGKKIDII
                     NTGSWTSKSIKEAKKVGEVNVAATTESENFMRLPTQAELKLTPDAAYVHMCANNTIFG
                     TEYTTLPDTKGVPLVSDMSSNIASREIDVSKHALIFAGAQKNLGPSGVTLVIIRKDIA
                     ERADKNLPTMLQYRTYIENNSLYNTPPTFGIYMLALTMEHIQDIGGLKAVETKNIAKA
                     KTLYDAIDGSNGFYRSPVNKTDRSRMNVNYRIYKGDTAHEDLEKQFIKEAEAAGLTGL
                     KGHRSVGGLRASIYNAVEQNSINELVKFMQDFARKNG"
BASE COUNT          226 a          241 c          281 g          319 t
ORIGIN      
        1 acgtgtttat ccttgaggaa atttttgaga aaagcacgca cctcttttat tgttggtttt
       61 gcaggcgagc caagattaac aacgagaagg ggcaacttct ttgggagagt cccccattta
      121 cttttagccg tttttgcgcg caaagtcttg catgaacttt acaagctcgt tgatcgagtt
      181 ttgctcaacc gcgttgtaaa tcgatgccct caaaccgccg acagaacggt ggcctttaag
      241 acccgtaagc cctgccgctt cggcttcttt aatgaattgt ttttccaaat cttcatgagc
      301 ggtatcgcct ttgtagatgc ggtagttgac gttcatgcgt gaacggtcgg ttttattcac
      361 tggggaacga taaaagccgt ttgagccgtc gatcgcgtcg tagagagttt tggctttcgc
      421 gatattcttg gtttccactg cttttaatcc gcctatgtct tggatgtgtt ccatggtgag
      481 tgcgagcata tagatgccga acgtgggtgg ggtgttgtag agcgagttat tttcaatgta
      541 cgtgcggtat tgcagcatgg taggtaaatt tttatccgcg cgctcagcga tgtctttgcg
      601 gatgatgaca agtgtcaccc ccgatggtcc caggtttttc tgcgcgccgg caaaaataag
      661 cgcgtgcttg gaaacgtcga tttcccgcga ggcgatattc gacgacatat cactcacgag
      721 gggaacgcct ttggtgtcgg gtagggtggt atactcggtg ccaaaaatag tgttgttcgc
      781 gcacatgtgt acgtacgcgg catcgggggt gagcttcagt tccgcttgcg taggcaggcg
      841 cataaaattt tcagattctg tggtggccgc cacgttgacc tcgcccactt tttttgcttc
      901 tttgatagac ttgctcgtcc atgagccggt gttgataata tcaatttttt tacccggaac
      961 attgaggttc atcggcacca tgctaaactg caaacttgca ccgccttgga taaagagcac
     1021 tgcgtattcg tcgggcacac ctaacgttgt tttaatgcca tctacgg
//
LOCUS       DAOMPD010000717         1067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_47948_length_1067_cov_1.770751, whole genome shotgun sequence.
ACCESSION   DAOMPD010000717 DAOMPD010000000
VERSION     DAOMPD010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47948_length_1067_cov_1.770751"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLY93_15720"
     CDS             <1..293
                     /locus_tag="PLY93_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRP70971.1"
                     /translation="GRDMGTEVFPEAHFKFFLTATSRVRAQRRLAEFRAKNKDITLDE
                     VERQIITRDNDDAQRTVGALKSASTAIFIDTSSQTQKEVVSTMLAYIRYAAI"
     assembly_gap    413..422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <423..>1067
                     /locus_tag="PLY93_15725"
     CDS             <423..>1067
                     /locus_tag="PLY93_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRP70972.1"
                     /translation="EFAIIDMGGKSEGRIGLREFDTAPTVGDSIEALVKSYDRETGLV
                     QLSKRELESRRGWEIVKEAYEKNVPVNGLVKRSMRDGYMLNVEGVFMFMPHTQVGLLG
                     PRERGAKRINIIGQKYLVKITSLNERRKTGEVSRKIFQDEQNATVWKEVGAKIRIGDI
                     VNGIITKHTNSGVFVNVEGIEGFLHQSNLTWERKTDSAKEKLEVGKEVSVRILE"
BASE COUNT          311 a          231 c          276 g          239 t
ORIGIN      
        1 acgggcgcga catgggtacc gaggttttcc ccgaggcgca ttttaagttt tttttgacgg
       61 cgacgagccg tgtacgggca caacggcgtt tggccgagtt tagagcgaaa aataaggata
      121 ttactttaga tgaagtcgag cggcagatta tcacacgaga taacgacgat gcgcagcgta
      181 cagtgggtgc attaaaatcg gcctcaacag ccattttcat tgacacctcg tctcaaactc
      241 aaaaagaagt agtaagtaca atgcttgcat atattcggta tgcggcaatt taggggtttc
      301 catggcaacg cgcaaagttc tctttcaaga tagcaacgtc tcaatggcag atgttttcaa
      361 aaccgatact gaagttgccg atatacgccc cggcggtgaa gttaccggta aannnnnnnn
      421 nncgaattcg ccattatcga catgggaggg aagtcggaag gccgcatcgg cttacgtgag
      481 tttgatacgg cgcccacggt cggcgactct atcgaagcat tagtcaaatc gtacgaccgt
      541 gaaacaggct tggtgcaact ttctaagcgc gagcttgagt cgcgccgtgg ttgggaaatc
      601 gtcaaagagg catacgagaa aaacgtaccg gtgaatggac ttgtcaaacg ctcgatgcgc
      661 gacggctata tgctcaacgt cgaaggcgtt ttcatgttca tgccgcacac tcaagtgggt
      721 cttttaggtc cacgcgaacg cggtgcaaaa aggattaaca tcatcggtca aaaatactta
      781 gttaagatca catcgctgaa cgaacgccgc aagaccggcg aagtctcacg aaaaattttc
      841 caagacgaac aaaacgcaac cgtttggaaa gaagtgggcg ctaaaattcg catcggcgac
      901 atagtgaatg gtatcatcac gaagcacact aactcaggcg tgtttgtaaa tgttgaaggt
      961 attgagggct ttttacacca aagcaattta acatgggagc gcaaaaccga cagcgccaaa
     1021 gaaaaactcg aagtgggtaa agaagtttct gtgcgcatat tggaaat
//
LOCUS       DAOMPD010000718         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_48043_length_1065_cov_2.438614, whole genome shotgun sequence.
ACCESSION   DAOMPD010000718 DAOMPD010000000
VERSION     DAOMPD010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48043_length_1065_cov_2.438614"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1065)
                     /locus_tag="PLY93_15730"
     CDS             complement(<1..>1065)
                     /locus_tag="PLY93_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRP70973.1"
                     /translation="SGKTLCYLLPILEAKLKNPKGKHLFMFPTKALAQDQLATYRAYS
                     KSLKAQWNINTFDGDTPPEERRQVRKAGDFILTNPDMLHSGIMPHHSVWKSFFENLET
                     IVIDEMHTYIGVFGSHVANVLRRLNRLCAHYGSHPRYIFCSATIANPAELASSLAERE
                     FTAITQSGAPEGKKYFLFYSPRYFENLQLVESPYKAAARLGAGLIQNSIATIFFARSR
                     NRVELLTQFIRARLPEHLRKNVRGYRGGFLPLERRQVEKSLRDGDVLGVVSTNALELG
                     IDIGSLNAVVAVGYPGRLSSLYQQFGRAGRQKETSLAILVASPSALDQYLLRNPDFIF
                     QSKGEAAIVNPDNLLIYQDQI"
BASE COUNT          266 a          250 c          300 g          249 t
ORIGIN      
        1 aatttggtct tggtagatga ggaggttgtc ggggttcaca atcgccgcct cgcctttgct
       61 ttgaaaaata aaatcgggat tgcgcaacag gtattggtcg agcgccgaag gcgacgctac
      121 cagaatggcg agcgacgttt ctttttgtct gcccgcacga ccaaactgtt gatagagcga
      181 gcttaaacgc ccagggtaac ctaccgcgac cactgcattg agcgagccga tgtcgatacc
      241 caactcgagt gcattcgtgg agacaacacc cagcacgtcg ccgtcgcgca acgatttttc
      301 aacctggcgg cgttcgagcg gcaagaaacc accacggtag ccgcgcacgt ttttgcgtaa
      361 atgctcgggt aagcgagcac ggatgaattg cgtcaagagt tcaacacggt tgcgcgagcg
      421 tgcaaaaaaa attgtggcaa tggaattttg gatgagccct gctcccaaac gcgcggcggc
      481 tttgtaggga gattcgacga gttgtaaatt ttcaaaataa cgcgggctat aaaaaagaaa
      541 atatttttta ccctcgggtg cacccgattg ggttatggcg gtaaactcgc gctcggcgag
      601 cgaagaggca agctctgcgg gattggcaat cgtcgccgag caaaaaatat aacgcgggtg
      661 cgaaccgtag tgcgcgcaaa gacggttgag ccgccgtaac acgttggcga cgtgtgaacc
      721 aaaaacaccg atgtacgtgt gcatttcatc gatgacgatg gtttccaaat tttcaaaaaa
      781 agacttccag acgctgtggt gtggcataat ccccgaatgc agcatatcgg ggttggttaa
      841 aataaaatcg cccgctttgc gcacttgtcg ccgctcttcc gggggggtgt cgccgtcgaa
      901 tgtattgata ttccattgtg cttttagtga ctttgaatac gcacggtacg tggcgagttg
      961 gtcctgcgcc aacgctttgg tggggaacat aaaaaggtgc ttacccttgg gattcttgag
     1021 ttttgcttct aaaatgggca ggagataaca aagcgttttc cccga
//
LOCUS       DAOMPD010000719         1062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_48193_length_1062_cov_2.394240, whole genome shotgun sequence.
ACCESSION   DAOMPD010000719 DAOMPD010000000
VERSION     DAOMPD010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48193_length_1062_cov_2.394240"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1062
                     /gene="miaB"
                     /locus_tag="PLY93_15735"
     CDS             <1..>1062
                     /gene="miaB"
                     /locus_tag="PLY93_15735"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRP70974.1"
                     /translation="QMNEYDSLLASKILESEATSTKDAAEADIILINTCAVRENAHQK
                     VYNRLREFGHLHKRGAKIGVLGCMAQNLREDLLYENLPIDFIMGPDGLRNLSTAVQHA
                     ESGEKKESFLNLSRTETYDDIVPSFEHHMRDRDNKTTAFVTIQRGCNNFCAFCVVPYT
                     RGRERSRPMGSIVDEINKLVEHNVKSVVLLGQNVNSYAYEKARFIDLIETLLVKTTIE
                     RIYFTSPHPKDFPIDLIELMAHEPRFCNQVHLPLQAGSDATLKRMKRNYTRSDFLKIV
                     ENFRTRVEDVAISTDVIVGFPGESAGDFEETLEVMQLADFDSAFMFAYSERKGTIAKR
                     LYPDDVPEEEKKSRIARLI"
BASE COUNT          283 a          245 c          268 g          266 t
ORIGIN      
        1 gccaaatgaa cgaatacgat tcgttgttgg cgagtaaaat tttagaaagt gaagcgacgt
       61 cgaccaaaga tgccgccgag gccgacatca tccttatcaa cacatgcgcg gtacgcgaga
      121 acgcgcacca aaaggtctat aaccggttgc gtgagtttgg gcatttgcat aagcgtggcg
      181 ccaaaattgg cgttcttggc tgtatggctc aaaatttgcg cgaagattta ctctatgaaa
      241 atttaccgat tgattttatc atgggccccg atgggctgcg taatctttcg acggcagtcc
      301 agcacgccga aagcggcgaa aaaaaagaaa gtttcttgaa tctttcacgc acagaaactt
      361 atgacgacat tgtgccgtcg ttcgagcacc acatgcgcga ccgcgataac aaaacaaccg
      421 cgtttgtgac aatccagcgc gggtgcaata atttttgtgc tttttgtgtg gtaccttaca
      481 cccgtggacg tgagcgttcg cgccccatgg gaagtattgt ggatgaaatc aataagctcg
      541 ttgagcataa cgtgaagtcg gtggtgcttt tggggcagaa cgtgaattcg tatgcgtatg
      601 aaaaggcgcg ctttatcgac ctcatcgaaa cgctattggt taaaacaacc atcgagcgga
      661 tttattttac ctcgccgcac cccaaagatt ttccgatcga tctcatcgag cttatggcgc
      721 acgaaccgcg cttttgcaac caagtgcact tgccgctgca ggcaggctcg gatgcaacgc
      781 tcaaacgaat gaagcgtaat tacacgcgct ctgatttctt aaagattgtt gaaaatttcc
      841 gtacacgcgt cgaagatgtt gcgatttcaa ccgatgtcat tgtaggattc cccggcgaaa
      901 gtgcgggtga ttttgaagag acgctcgaag ttatgcagct tgccgatttt gattcggcgt
      961 ttatgtttgc atactctgaa aggaaaggca cgattgctaa acgcctttat cccgacgacg
     1021 tgcccgagga agaaaaaaaa tcacgtatag cgcgcctcat tg
//
LOCUS       DAOMPD010000720         1061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_48257_length_1061_cov_2.312127, whole genome shotgun sequence.
ACCESSION   DAOMPD010000720 DAOMPD010000000
VERSION     DAOMPD010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48257_length_1061_cov_2.312127"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLY93_15740"
     CDS             complement(<1..443)
                     /locus_tag="PLY93_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRP70975.1"
                     /translation="MQHNFQVHVESVEVNGQKYELNTGKWARQADGSVLLQWGDMLLM
                     ANATGAKKAGEGLDFFPMTVEYREKYYAAGKFPGGFIKRENRPSDKEILISRVIDRPI
                     RPLFPDNFTNEVQIFITLLSAQIGYSADVHAVTAASAALSISGLP"
     gene            complement(623..892)
                     /gene="rpsO"
                     /locus_tag="PLY93_15745"
     CDS             complement(623..892)
                     /gene="rpsO"
                     /locus_tag="PLY93_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRP70976.1"
                     /translation="MMLATEEKKAVIASHQKSSKDTGSVEVQVALLTEKINRLTGHFQ
                     THVKDHHSRVGLLKMVGKRRRLLDYLRRNSVDRYKALIGKLNLRK"
BASE COUNT          262 a          249 c          263 g          287 t
ORIGIN      
        1 aacggcaaac cagaaatact gagtgcagcc gacgcagcgg tgacagcgtg gacatcggcg
       61 ctatagccta tttgcgcgct caagagtgtg ataaaaattt gcacttcgtt ggtgaagtta
      121 tcggggaaaa gtgggcggat aggacgatcg ataacgcgtg aaatgagaat ttctttgtcg
      181 gaaggacggt tttcacgctt gataaaacca ccgggaaact ttcctgcggc gtagtacttc
      241 tcgcgatact ctaccgtcat ggggaagaaa tctaaccctt caccggcttt cttggcgccg
      301 gtggcgttcg ccatgaggag catgtcgccc cattggagca gtaccgagcc gtcggcttga
      361 cgtgcccact tacctgtatt taattcatat ttttgaccgt tgacttcgac gctctcaacg
      421 tgtacctgaa aattgtgttg cattgtatat cctttgtgtt tggtggaatg caattaacaa
      481 agaaaacgca acatccggct atggatttac ccaagagaaa gtttcatcac tgcctacgca
      541 gttcattaac taacccccgt aatatccgac gcttcgggtg gagcgaatca aagtgaccgc
      601 aatcgcacga caggggaagg tattatttac ggagatttag cttgccgatg agcgctttat
      661 agcgatccac gctgttgcgg cgcaagtaat ccagtagacg gcgacgtttg cccaccatct
      721 ttaaaagacc tacacgcgaa tggtgatctt tgacgtgtgt ttgaaaatga ccagttagac
      781 ggttaatctt ttcagtcaaa agtgcaacct gtacttcgac actgccggtg tctttagagc
      841 ttttctggtg tgacgcaata actgctttct tttcttcggt tgcgagcata atttccttta
      901 ctttaacttt cccttttgtt tgcggcagat tgagcaagtc gaacaggctg acaagcgttt
      961 ctatagaatt ggaagcgcac aagagcctgt acgccaactt gctgcccgag ttccggccta
     1021 tccgctataa cacacaggga gcatcggtgt ggctcatgct t
//
LOCUS       DAOMPD010000721         1057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_48477_length_1057_cov_4.223553, whole genome shotgun sequence.
ACCESSION   DAOMPD010000721 DAOMPD010000000
VERSION     DAOMPD010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48477_length_1057_cov_4.223553"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLY93_15750"
     CDS             <1..444
                     /locus_tag="PLY93_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRP70977.1"
                     /translation="VDFALSPEMISEAAYVNRYLIEDQIVPVVSKDFPAASISDWQNL
                     KRCDWILFHSGSAIRKISNQIFRDTERNFVPRVVMEFRSVPSLVRLLEEGVGVGFISK
                     LSLSNKLMVIPLTHLHRKRRFFISYKKKHEQLAGVIETVLRCGGG"
     gene            complement(441..1004)
                     /locus_tag="PLY93_15755"
     CDS             complement(441..1004)
                     /locus_tag="PLY93_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70978.1"
                     /translation="MRFFDMTPRRRTSLSIRSASRLRVKAAVILLNARGYEFSEQSLY
                     RECLTLVLQFWKGRGGIAQRNTKYNPRAGEYEVVPFYTTEHLRRTAHARGHHAGISIS
                     RMVDFGIAVYLERIIERVLSRMHHAEFAHEVSFWKARYEKRHNRGAMIINYSHWTAKP
                     QNSSLKFGEEVPITPWPKDKLVIFMES"
BASE COUNT          275 a          247 c          243 g          292 t
ORIGIN      
        1 gtggattttg cgttaagccc tgaaatgatt agcgaagcgg cgtatgtgaa ccgttacctt
       61 atcgaagatc aaattgtgcc ggttgtctct aaagattttc ctgcagcatc cattagtgat
      121 tggcaaaatc taaaacgatg cgattggatt ttattccaca gtggctcggc gattcgtaaa
      181 attagcaatc aaatttttcg ggacacggag agaaattttg ttcctcgcgt ggtaatggaa
      241 tttcgtagcg tacctagtct cgtgcgcctt ttggaagagg gagtgggagt gggatttata
      301 tcgaagcttt cactctccaa caagctcatg gtgatacccc tcacacatct tcaccgcaaa
      361 agacgcttct ttatatcgta caagaaaaag catgagcagc tcgcaggggt gattgagact
      421 gtgctgcggt gtggtggtgg ttaggattcc atgaagatca cgagtttgtc cttcggccac
      481 ggggtgattg ggacttcttc gccaaatttt aggctggaat tttgtggttt tgctgtccaa
      541 tgtgagtagt taataatcat cgcaccccga ttgtggcgtt tctcgtatct tgctttccaa
      601 aaagaaactt cgtgtgcaaa ttctgcgtgg tgcatccgac tcaacacacg ctcaataatc
      661 ctctctaaat acactgcaat cccaaaatcc accatccgcg aaatcgaaat ccccgcatgg
      721 tgtccccgcg cgtgggcggt ccgccgcaaa tgttccgtcg tataaaacgg cacaacctcg
      781 tactcacccg cgcgtggatt atattttgta ttgcgctgcg caataccacc cctccctttc
      841 caaaattgca gcaccaacgt aagacattca cggtagagcg attgctctga aaattcataa
      901 ccgcgtgcgt tgaggagtat cactgccgct ttcactctaa ggcggctggc agatcgaata
      961 ctcagcgaag tccgacggcg gggggtcata tcaaagaatc tcatatatat tataaatacc
     1021 agaaaacgct taatttttct cactttttga aaaaaaa
//
LOCUS       DAOMPD010000722         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_48828_length_1051_cov_2.952811, whole genome shotgun sequence.
ACCESSION   DAOMPD010000722 DAOMPD010000000
VERSION     DAOMPD010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48828_length_1051_cov_2.952811"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLY93_15760"
     CDS             complement(<1..188)
                     /locus_tag="PLY93_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HRP70979.1"
                     /translation="MPIFISKVSVQSAVFKENQAAYTIHEKRLEEMRAWVAPGGRAAA
                     LERHTSRGKLFARDRIQTL"
     gene            complement(189..>1051)
                     /locus_tag="PLY93_15765"
     CDS             complement(189..>1051)
                     /locus_tag="PLY93_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRP70980.1"
                     /translation="GLAITFDMMMTTTLLTVYLATRSTHGFRAVAAFVGFISIELAFL
                     VANLEKFPHGGWVTFLIGVILFVVMWSWYSARKIRNRLVEFVPLADYFDTLHELSHDT
                     SVSKYATNLVYLTSANNPSEIEWKVIYSLFYKQPKRADVYWLVHIDVTDEPYTMSYKV
                     DVLVPNDVIRIEFKLGFRVEPKVNQLFRKVVSVLVEEGIVDITSRYASLHQKNILGDF
                     RFIVFDRILSYDNELNVYERVVTRVYKILKWACPTEEKSFGLDTSIVEIERVPLIVST
                     PREFQLERLT"
BASE COUNT          287 a          268 c          231 g          265 t
ORIGIN      
        1 agtgtttgga tacggtcgcg cgcaaaaagt ttacctctac tcgtgtggcg ttcgagggct
       61 gctgcgcgcc ctccgggggc cacccatgcg cgcatttctt ctaaccgttt ttcgtggatt
      121 gtgtacgccg cttgattttc tttgaagaca gcgctttgta cgctcacttt tgatataaaa
      181 ataggcatct atgtcaacct ttctaattgg aattcccgtg gcgtcgacac aatgagcggc
      241 acgcgctcaa tttccacaat cgaggtgtct aaaccaaacg atttctcctc ggtggggcat
      301 gcccatttca aaattttata cacccgtgtc acaacgcgct cataaacatt gagttcgtta
      361 tcgtaactta aaattcggtc gaaaacaata aagcgaaaat ctcctaaaat atttttttgg
      421 tggagcgatg cataccgact ggttatatcg acaatccctt cttcaacgag caccgagacc
      481 actttcctaa aaagctggtt cactttgggc tccacacgaa agcccaactt aaactcgatg
      541 cgtataacgt cgttgggcac caatacatcg actttatacg acatggtgta aggctcgtcg
      601 gtcacgtcga tgtgtaccaa ccagtataca tcggcgcgct tgggttgttt gtaaaaaagc
      661 gaataaataa ctttccattc aatttcgctg gggttgtttg cgctcgttaa gtacacaagg
      721 ttagtggcat acttcgacac cgaggtgtcg tgactcaatt catgcaatgt atcgaaataa
      781 tcggcaagcg gtacaaactc aaccaagcgg ttccggattt tgcgcgcaga gtaccacgac
      841 cacatcacta cgaacagaat aacaccgatg agaaaagtca cccacccacc gtggggaaat
      901 ttctcgaggt tcgcaacaag aaatgctaac tcgatcgaaa taaaaccgac gaacgccgcc
      961 accgcccgaa aaccatgcgt cgagcgcgtc gctaaataca ccgtcaaaag cgtcgttgtc
     1021 atcatcatat cgaacgtgat ggcaaggccg t
//
LOCUS       DAOMPD010000723         1047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_49089_length_1047_cov_2.168347, whole genome shotgun sequence.
ACCESSION   DAOMPD010000723 DAOMPD010000000
VERSION     DAOMPD010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49089_length_1047_cov_2.168347"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>372
                     /locus_tag="PLY93_15770"
     CDS             <1..>372
                     /locus_tag="PLY93_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin-NADP reductase"
                     /protein_id="HRP70981.1"
                     /translation="CLVVKRFVAPHKDTGKTFRGVASNYLCDLKVGDEVLLTGPAGRK
                     FALPTPEEIHRPYIFIATGTGIAPFRGFLQRLYKHTPHFNSPVYLLFGVKTEKELLYH
                     DEFTEYKNPNFHYLKALSREM"
     assembly_gap    373..382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <383..594
                     /locus_tag="PLY93_15775"
     CDS             <383..594
                     /locus_tag="PLY93_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70982.1"
                     /translation="ARYYVGDLLHDHADELWPIISDERTIIYMCGLKGMEEGVSRAIN
                     AMAQKHGKGENYYATLDHRMEDEVY"
     gene            596..>1047
                     /locus_tag="PLY93_15780"
     CDS             596..>1047
                     /locus_tag="PLY93_15780"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HRP70983.1"
                     /translation="MKYLNKVFMRHSGIYPVALLITVALFLSACSIKKMVVRQATDFF
                     GESRKVFEQETDLELAETSIGSNLKLLEAMQVHDPENESLNLLIAEIYSVYTLSFVED
                     KVDAAEALHNDKEAARERVRAKMFYLRARDAAGRVLLPKLEIDNLSFLT"
BASE COUNT          279 a          251 c          248 g          259 t
ORIGIN      
        1 atgcctcgtg gtgaaacgct ttgtggcgcc gcataaagac accggcaaaa cattccgtgg
       61 tgtggcgtcg aattatctct gcgacttaaa agtgggcgat gaggtactcc tcacaggtcc
      121 tgccgggcgc aagtttgccc taccaacgcc ggaggaaatc catcgtccgt atatttttat
      181 cgccaccggt accgggattg cgccgttccg tggctttttg caacgactgt ataagcacac
      241 tccccatttt aactcaccgg tttatttgct ctttggcgtt aaaacagaaa aagaactcct
      301 ttaccacgac gagtttaccg agtacaaaaa tcccaacttc cactacttaa aagccttgag
      361 tcgtgaaatg aannnnnnnn nngcgctcgc tattatgtag gggatttact ccacgaccac
      421 gccgacgaac tctggcccat tatcagcgat gaacgcacca tcatttatat gtgcggtctc
      481 aaaggcatgg aagagggcgt gtcgagagcg atcaacgcca tggcgcaaaa gcacggcaaa
      541 ggcgaaaact actacgcgac gttagaccac cgcatggaag acgaagtgta ctaaaatgaa
      601 atatttaaat aaagtattca tgcgtcacag tggtatttac cccgtggcac ttttaatcac
      661 cgttgcactt tttttatcgg cgtgctccat caaaaaaatg gtcgtgcggc aagcgaccga
      721 ttttttcggt gaaagccgca aagtctttga gcaagagacc gatttggaat tggctgagac
      781 gtcgattggc tccaatctta aacttttaga agcgatgcag gtacacgatc ccgaaaacga
      841 aagtttgaat ttgcttatcg ctgaaatcta ttcggtgtat acgctctcgt tcgttgaaga
      901 taaggtcgac gccgccgaag ctctacacaa cgataaagaa gcggcgcgtg aacgggtgcg
      961 tgccaaaatg ttttatctac gcgcgcgcga tgcagcaggg cgggtactcc tgccgaaact
     1021 tgaaattgat aatctttctt ttttgac
//
LOCUS       DAOMPD010000724         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_49516_length_1040_cov_2.161421, whole genome shotgun sequence.
ACCESSION   DAOMPD010000724 DAOMPD010000000
VERSION     DAOMPD010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49516_length_1040_cov_2.161421"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLY93_15785"
     CDS             complement(<1..329)
                     /locus_tag="PLY93_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70984.1"
                     /translation="MKILRDKKCLITGAASGIGRATAIATAKEKAALFLTDINPKGLE
                     ETAEACRKIGATVIHHALDISDYKAVKAWAEEIHNKHGSLDVLMNIAGISIWGSVENL
                     THEDWRR"
     gene            complement(332..778)
                     /locus_tag="PLY93_15790"
     CDS             complement(332..778)
                     /locus_tag="PLY93_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70985.1"
                     /translation="MYRILLSIVAVLFLHCGTTQSIVLPEGFARYEKPKKDVKAITAD
                     GVRLFTARFENRPKGNLALWVESLSEQLTQTGYRVVDKKSIKAGSLEGVAIESQASYA
                     GQDFSYLVAIFVKDDNVFTVESAGERKAFDKIKPALLQSLTTFKGE"
     gene            complement(784..>1040)
                     /locus_tag="PLY93_15795"
     CDS             complement(784..>1040)
                     /locus_tag="PLY93_15795"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRP70986.1"
                     /translation="LSDVNKVLADADLEETLEVEKELDDIRGNIDALRGELQFLRERS
                     SLKKIIFYFESTESAPQPRDYESFSWLDKMNIPSLIERFQ"
BASE COUNT          237 a          248 c          240 g          315 t
ORIGIN      
        1 caccgccgcc aatcttcgtg cgttaaattt tcaacgctac cccaaatcga aatgcctgca
       61 atgttcatca acacgtcgag cgagccgtgc ttgttgtgga tttcctctgc ccacgctttg
      121 accgctttgt aatcggaaat atcgagtgcg tggtggataa cggttgcgcc tatcttgcgg
      181 cacgcctcgg cggtttcttc caagcctttt gggttgatgt cggttaaaaa aagcgccgct
      241 ttttctttgg cggtcgcaat cgccgtggct ctaccgatgc cgctggcagc tccggtgatg
      301 agacattttt tgtcgcgtaa tattttcatg gttattcccc cttaaatgtt gtgagtgatt
      361 gaagaagagc aggttttatt ttgtcgaacg ctttgcgctc gcctgcgctt tcgacggtga
      421 agacattgtc gtcttttaca aaaattgcga caagatacga aaagtcttgc cctgcatacg
      481 acgcttggct ttcgatggca acgccttcga gcgaacctgc tttgatggat tttttatcca
      541 ccactcggta accggtttgc gtcagttgtt ctgaaagcga ttccacccaa agcgctaaat
      601 ttcctttagg tctattttca aagcgcgcag tgaaaagtcg cacgccgtca gcggtaattg
      661 ctttcacatc ttttttgggt ttctcgtaac gcgcaaaacc ttcgggcaaa acaatcgact
      721 gtgtggtgcc gcagtgcaaa aaaagtacag cgacaatact taaaagaatg cggtacatag
      781 ttttcactgg aacctctcta ttaggctggg tatgttcatt ttgtccaacc acgaaaaact
      841 ttcgtagtcg cgcggttgtg gagcgctttc tgtactttca aaatagaaaa ttattttttt
      901 aagagaagaa cgttcgcgta aaaattgcag ttcaccgcgc aatgcgtcga tgtttccacg
      961 gatgtcgtcg agttcttttt cgacctcgag cgtttcttct aaatcggcgt cggctaaaac
     1021 tttattcaca tcgctcagtt
//
LOCUS       DAOMPD010000725         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_49520_length_1040_cov_1.974619, whole genome shotgun sequence.
ACCESSION   DAOMPD010000725 DAOMPD010000000
VERSION     DAOMPD010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49520_length_1040_cov_1.974619"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLY93_15800"
     CDS             <1..199
                     /locus_tag="PLY93_15800"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRP70987.1"
                     /translation="HVKTNVEADSVLVSGRVDGNIYALDFVHLMKNARVMGDIVAANL
                     LVDEGVIFEGRAKINHLNVGG"
     gene            275..745
                     /locus_tag="PLY93_15805"
     CDS             275..745
                     /locus_tag="PLY93_15805"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HRP70988.1"
                     /translation="MGQIAEARLSSFNNVGTEKNFSRVPTSVAQGASPASVAFSSLVA
                     QVLQRKESAQAKSFDELLADLTEEENDFTENPSREKFESYRKAVKALMDMLGKRSYRL
                     HNWEDKRRRRYEIVKVIDAHLAALYSSLMRRNQDVVITLHLMGQIRGLIFDLQA"
     gene            complement(749..>1040)
                     /locus_tag="PLY93_15810"
     CDS             complement(749..>1040)
                     /locus_tag="PLY93_15810"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70989.1"
                     /translation="AASAGYLAHADSRALGNLMVELGAGRKKSTDAIDDRVTLEMLKA
                     PGTEIKKGDAMARIYKPRLSPHEKKQIDHAAKMAFSITKGKPKPIRNILKTI"
BASE COUNT          249 a          240 c          273 g          278 t
ORIGIN      
        1 gcatgtcaaa acaaacgtgg aagccgacag tgttttagtt tcaggacggg tggatggcaa
       61 catctacgcg ttagattttg tgcatttgat gaaaaatgcg cgcgttatgg gagacattgt
      121 cgctgcaaac ttgcttgtcg acgaaggcgt gatatttgag gggcgtgcaa aaattaacca
      181 cctcaatgtg ggaggttaaa caagttagat gacctcttgt gtctatatag gcgcataact
      241 ctcatttttt tggggaatat gccgatagtc gactatgggg caaattgccg aggcacggct
      301 ttcttctttt aataacgttg gcacagagaa aaatttttcc cgcgtcccca caagcgtcgc
      361 gcagggggca tcccctgcgt cggttgcttt ttcatcgctg gtggcgcagg ttttgcaacg
      421 caaagagagc gcacaggcca aaagcttcga cgagctcctc gccgacttaa cagaggaaga
      481 aaatgatttc accgaaaacc cttcgcgcga aaaatttgaa tcgtaccgca aagcggtcaa
      541 agcattgatg gacatgctcg ggaagcgcag ttatcgttta cataactggg aagataaacg
      601 ccgccgtcgc tatgaaatcg taaaggtcat cgatgcgcat ttagcggcgc tgtattcctc
      661 gctcatgcgg cgcaaccaag acgtggtgat tacactacac ctcatggggc agatacgcgg
      721 tttgattttt gatctacaag cgtaagcgct aaatagtttt taagatatta cgtatgggtt
      781 ttggtttgcc ttttgtaatg gagaatgcca tttttgccgc gtggtcgatc tgtttttttt
      841 catggggact aagtcgtggt ttgtaaatcc gcgccatcgc atcgcctttt ttgatctctg
      901 tcccgggcgc tttcaacatt tcgagcgtga cacggtcgtc aatagcgtcg gtggatttct
      961 tacgccctgc gcccaactcg accatcaggt tgcccagcgc acgcgagtca gcgtgcgcta
     1021 aatagcccgc gcttgcagcg
//
LOCUS       DAOMPD010000726         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_49576_length_1039_cov_2.634146, whole genome shotgun sequence.
ACCESSION   DAOMPD010000726 DAOMPD010000000
VERSION     DAOMPD010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49576_length_1039_cov_2.634146"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..876
                     /locus_tag="PLY93_15815"
                     /pseudo
     CDS             181..876
                     /locus_tag="PLY93_15815"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
     gene            complement(826..>1039)
                     /locus_tag="PLY93_15820"
     CDS             complement(826..>1039)
                     /locus_tag="PLY93_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRP70990.1"
                     /translation="TAYKSGVFDEGANSGGVNHAVNIVGWDNSKRAWLIRNSWGTNWG
                     DQGYAWVEWGSNLIGYGTMWVEPANL"
BASE COUNT          279 a          247 c          216 g          297 t
ORIGIN      
        1 gaaattatcg aacgcgactt ggatttcaca cgcttattgc accggaaact cttgccgtct
       61 gcactccctc gcaacgagtt cttagattgt agcatttttt atcaaagcgt cgacaaagtg
      121 ggcggtgatt tttatgactt tataaaaaca cagaactcgt ggcgcatcat tctcggcgac
      181 gtgagtggcc atggtgtttc cgccggcttt ttttcagcga tggcatcgct cattttgcgc
      241 gatttttgga aagatttcaa gcacagttta aacgacgcag cgcaatactt aaacgctgaa
      301 attttacgtt attccgcaaa tggtattttt atcagcgcta tttttgtgga aataaatttt
      361 ttaaccggtg aaacgacact catcaacgca gggcatcccc ccatttttca tttaaaaaag
      421 aactgtgtca attcgttaat gatacccgga cgactcttag gagcctgtgc gccaactcat
      481 tttttgccat tttttaacgc caaaaaggag cttaacttcg tataacttag cgtagcgtaa
      541 gttaaatttt tgattttaga gttgtcgcac aggctcttag gcgtcgtcgc cgacagtatt
      601 tttaatccga agacaatcac actagagcat ggcgatacgc taatcctcta taccgacggc
      661 cttctggaaa caacgaacga gaacggcgaa gagtttcaag aaaataagtt aaaagaagtt
      721 ttgaacgctt cgcttgtgct atctttagac caacgcgaga aacatcttct aacagaactg
      781 cacgcttttc gcgacaaaat tccgttagaa gatgatttaa ctttattaca aattcgccgg
      841 ttcaacccac atcgtgccat agccaatcag gtttgagccc cactcgaccc atgcatagcc
      901 ttggtcaccc cagtttgtgc cccatgaatt acggatgagc cacgcacgtt tggagttgtc
      961 ccaaccaacg atattgacag cgtggttgac gccccctgag tttgcccctt cgtcgaagac
     1021 gccgctttta taagcggtg
//
LOCUS       DAOMPD010000727         1038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_49628_length_1038_cov_3.400814, whole genome shotgun sequence.
ACCESSION   DAOMPD010000727 DAOMPD010000000
VERSION     DAOMPD010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49628_length_1038_cov_3.400814"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLY93_15825"
     CDS             <1..794
                     /locus_tag="PLY93_15825"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRP70991.1"
                     /translation="HVVVAMLYFSRKICIIPGFVFFTSVNPAVLFAQSLEVEGEEQTS
                     PETDIPEKSTLDGRIAAGYSYSYFNRVSALRQQNSVLWRKGKNSLRGFNIISGGMGSM
                     QGKFNAFFSGSADHYFTNWIEGFLFSTYDANAVTNMQGKLIVGGGLKTVIIRSKLFLF
                     DFSLAPTYSQTKYSNLPTVSNPTLSVRTRIRLQLTQNLSFALPYFVIYNSAGANDQWH
                     SLDPSFNFKMNSTSDLSCGYWYRYDSFSNQYSGTFYATAGVIFNN"
BASE COUNT          293 a          224 c          215 g          306 t
ORIGIN      
        1 aacatgtcgt tgtggcaatg ctctattttt caagaaaaat ttgcattatt ccaggctttg
       61 ttttctttac atctgttaat cccgctgtgc ttttcgcaca aagtctggaa gttgaaggcg
      121 aagagcagac atctccagaa accgacattc cggaaaaaag cactttggat gggcgtattg
      181 cggcaggcta ctcctattca tattttaacc gcgtgtcggc gctgcggcag caaaattcag
      241 tactgtggcg caaaggcaaa aattcattga gaggatttaa tataatctca ggcggtatgg
      301 ggtcgatgca aggcaaattc aacgcatttt ttagcggtag cgcagatcac tattttacga
      361 actggataga ggggtttcta ttttcaacgt acgacgcgaa tgccgtcacg aatatgcagg
      421 gcaaattaat tgtgggtggt ggacttaaaa ccgttatcat acgttcaaaa ttatttcttt
      481 tcgatttttc gctcgcacca acgtattcac aaacaaaata tagcaattta ccgacagtgt
      541 caaacccaac cctctcggtg cgcacgcgta ttcgtttaca gctaacgcaa aatttaagct
      601 tcgctcttcc ctactttgtt atctataact cagccggagc taacgaccaa tggcattcgc
      661 ttgacccttc tttcaatttc aaaatgaata gcacttccga tttaagctgt ggttattggt
      721 accggtacga ttcattttca aatcaatact caggtacatt ttatgccact gcaggtgtta
      781 tatttaataa ctaatcttct gagtaattgt agaactatcg agcctgtgca ccagctcgcg
      841 caacgtgtac aagaagagaa ttttttttgt aaatttttgt atgcggttcg ctcaaaacac
      901 aaaattttgc acaaattcgg gcgccttcgc cccgtgcaga gcaaattgga acaaattgcc
      961 gtcggcctca cggcattgca cgccgctttg gcggaggaat ttgtgcaaaa tagcgtacaa
     1021 aaatttgcaa aaaaattg
//
LOCUS       DAOMPD010000728         1034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_49931_length_1034_cov_1.715015, whole genome shotgun sequence.
ACCESSION   DAOMPD010000728 DAOMPD010000000
VERSION     DAOMPD010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49931_length_1034_cov_1.715015"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /gene="hslU"
                     /locus_tag="PLY93_15830"
     CDS             complement(<1..>1034)
                     /gene="hslU"
                     /locus_tag="PLY93_15830"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRP70992.1"
                     /translation="LKPVEIVRHLDEFIIGQKDAKKAVAIALRNRERRLALGEHPLRE
                     EIHPKNIIMIGPTGMGKTEIARRLARLANAPFIKVEATKYTEVGYVGRDVESMIRDLV
                     MIAIKIVRSEFEEVSKAEAERRTEERLLDLLLSGKTDKPENNSTALASTDDEDEKIAR
                     DIFRKKLKAGQLEDREVELKMPQQAGPQVAVQMMAIPGMEELENQMQNVLGDIFPKKK
                     ERKKLTIVEARKALYQEELDALIDFDKIKEEAIKRTENTGIVFIDEIDKIASRGGKGS
                     PDISREGVQRDILPLVEGSTVNTRHGQVKTDHILFIAAGAFHIASVTDLIPELQGRFP
                     IRVELKPLLR"
BASE COUNT          219 a          262 c          243 g          310 t
ORIGIN      
        1 cccgcaaaag cggtttgagc tccactcgaa tcggaaaacg gccttgtagt tcggggataa
       61 gatccgtcac cgatgcaatg tggaacgcac ctgcggcgat aaaaagaatg tgatcggttt
      121 ttacttggcc gtggcgggtg ttcactgttg aaccttcgac aagcggtaaa atatcgcgct
      181 gcacgccttc gcgggaaatg tcgggcgagc ccttgccacc tcgactggca attttatcga
      241 tttcgtcgat aaacacaatg ccagtgtttt cggtacgttt gatcgcttct tctttaattt
      301 tatcaaagtc aataagcgcg tcgagttctt cttggtagag agctttgcgc gcctcgacga
      361 tggtgagttt tttacgttct tttttcttcg ggaaaatatc gcccaaaaca ttttgcattt
      421 ggttttcgag ctcttccatg cctgggattg ccatcatttg cacagcgact tgtggccctg
      481 cttgctgtgg cattttcaac tcaacttcac ggtcttcgag ttggcctgct ttaagtttct
      541 tgcgaaaaat atcacgcgca attttttcgt cttcatcgtc agtactcgcg agcgcagtcg
      601 aattgttttc gggtttgtcg gttttccccg agagcagtaa atccaacagc cgttcctctg
      661 tgcgccgttc ggcttctgct tttgatacct cttcaaattc agagcgcaca attttaatcg
      721 caatcatcac caaatcgcgg atcatacttt ccacgtcgcg ccccacatac cccacttcgg
      781 tgtacttggt cgcttcgact ttgataaaag gcgcgttggc aaggcgcgcc aaacggcgtg
      841 caatctcggt tttacccata ccggtggggc cgatcataat gatattttta ggatgaattt
      901 cttcgcgtaa cgggtgctcg cccaacgcta aacgacgctc tcggttacgc aaagcaattg
      961 ccaccgcttt ctttgcgtct ttttgaccga tgatgaactc atcgaggtgg cgtacaattt
     1021 ctacgggttt aagt
//
LOCUS       DAOMPD010000729         1030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_50207_length_1030_cov_1.587692, whole genome shotgun sequence.
ACCESSION   DAOMPD010000729 DAOMPD010000000
VERSION     DAOMPD010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50207_length_1030_cov_1.587692"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1030
                     /locus_tag="PLY93_15835"
     CDS             33..>1030
                     /locus_tag="PLY93_15835"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70993.1"
                     /translation="MPDFVLTYAGKAGSRVIVNQVEQALPTKIHSIRVASGVTMSIDY
                     KPATQTPNITCPAAGTCTGPQGKTLDTTHGIANPTPRYLVTRVTTTSDRDLDGDGVPD
                     TFAQAYEYYNGRVSTGYVHERANLGFEKIKTTDVNSGNYLITTYRQDKPFHGQAVVSR
                     SYLANDELISEEISATPQQWLCDELGCVHDQTNNPNPTQPRQLRFSGPAETLTYENGI
                     LIGRKTEQVINYDNYGNPVATKSGVEANGSQHNVYKFTTYLNETSATRAIGLPVSEIL
                     CYSSSECIVGDNNFISAGRAYYDNGGLGTVGVRHLATRKEKYVIADAGAGAWVAEE"
BASE COUNT          281 a          242 c          264 g          243 t
ORIGIN      
        1 ggtctaattt tttggatatg aacggcgacg gcttgcccga ctttgtactg acttacgcag
       61 gcaaagcggg gtcgcgtgtt atcgtaaatc aagtcgaaca agctttgccg accaaaattc
      121 acagtatacg agttgcttcc ggtgtcacta tgagcatcga ctacaaacct gctactcaaa
      181 cccccaacat tacttgcccc gctgctggca cttgcaccgg cccacaaggc aaaacgctcg
      241 acaccaccca cggcattgcc aaccccaccc cgcgctatct tgtgactcgt gttaccacaa
      301 cgagcgatcg tgatttggac ggcgatggtg tacccgatac gtttgcacaa gcttacgaat
      361 actacaacgg tagggtcagc acggggtatg tgcatgagag ggcgaatctc ggatttgaga
      421 agataaaaac cacggatgtc aattcgggga attatttgat caccacgtat cgccaagata
      481 agccgtttca cgggcaagcg gtcgttagca gaagttattt agccaatgat gagttgatta
      541 gcgaagaaat ttctgcgaca ccgcagcagt ggctttgcga tgagttgggt tgtgtacacg
      601 accagacaaa caatccgaat ccaacgcagc cgagacaact tcgcttttca ggcccggctg
      661 agactttaac ttatgagaat ggaatcttga tcggtcgcaa gaccgagcag gtaataaatt
      721 acgacaatta cggtaatcct gttgccacta aaagcggtgt agaggcaaac gggagtcagc
      781 acaacgtcta taaattcacc acttatttga atgagacaag cgccacacgg gcaatcggct
      841 taccggtttc tgaaatacta tgttactcat cgagcgagtg tattgtaggt gataataatt
      901 ttatttcagc cgggcgtgct tattacgata atggcggcct gggtaccgta ggtgtacgtc
      961 atttggcgac gagaaaagaa aaatacgtaa tcgcagatgc tggggcgggc gcttgggttg
     1021 cggaagagtt
//
LOCUS       DAOMPD010000730         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_50243_length_1029_cov_2.386037, whole genome shotgun sequence.
ACCESSION   DAOMPD010000730 DAOMPD010000000
VERSION     DAOMPD010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50243_length_1029_cov_2.386037"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLY93_15840"
     CDS             <1..712
                     /locus_tag="PLY93_15840"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRP70994.1"
                     /translation="YPNCIKPQRDSVQMPAYHHKVENQNGITLVNIGVGRANAKTITD
                     HIAVLRPDLMVMVGHCGGLRNHQNIGDYVLASAYMRADGVMHDVLPANVPVLPNFLIN
                     LALRHELENKKIPYRMGTVFTTNNRNWEFNQKATIDAMKLSRSIAVDMESATIAANGY
                     RYRIPHATLLCVSDKPLHGKPKLSNEAQSFYQDSKEKHLEIAIHTVEILRQRFPEGLP
                     NDDMRSFDEPLFGSAGKD"
     gene            complement(723..>1029)
                     /locus_tag="PLY93_15845"
     CDS             complement(723..>1029)
                     /locus_tag="PLY93_15845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRP70995.1"
                     /translation="KETATRIRAALRENEIFGRIGGEEFAIFIPQVTRENAVLLAERV
                     RVVVSGLPYKLESASIPVTLSVGVCHATGRTPLEKFLERADKALYKSKRTGRNKVTI"
BASE COUNT          273 a          271 c          232 g          253 t
ORIGIN      
        1 ttatcccaac tgcataaagc cacaacgcga cagtgtgcaa atgccagcat accatcataa
       61 agtagaaaac caaaatggga ttaccctcgt taatatcggt gttggccgag cgaacgcaaa
      121 aactattacc gaccacatcg cggtgttacg ccccgatctc atggttatgg ttggccactg
      181 cggcggttta cggaaccatc aaaatattgg agattatgta ctcgcctcgg cgtacatgcg
      241 cgccgacggt gtgatgcacg atgtgttgcc tgcaaatgta ccagtgcttc ccaatttcct
      301 catcaatttg gcgctgcggc atgaactgga aaataaaaaa attccttacc gcatggggac
      361 agtctttacg actaacaatc ggaattggga atttaatcaa aaagcaacca tcgatgcgat
      421 gaaactctct cgttcgattg ccgtcgatat ggaatccgcc acgattgccg ccaacggtta
      481 ccgctaccgc atcccccacg cgacgctgtt gtgcgtgagc gataaaccat tacacggtaa
      541 gcccaaactt tcaaacgagg cgcaatcgtt ctatcaagac agtaaagaaa aacaccttga
      601 gattgccatc cacaccgttg aaattttacg gcagcgtttt ccggagggac tccccaacga
      661 cgatatgcgc tcgtttgacg aaccgctttt tggtagtgct ggcaaggatt aaagtagaag
      721 ttctatatcg tcactttatt gcggccggta cgtttacttt tatagagggc tttatcggca
      781 cgctccaaaa atttttcgag tggtgtgcgt ccggtggcat ggcatacccc cacactcagc
      841 gttacgggaa tcgatgcact ctccagttta tacggtaaac cgcttacgac aacgcgtacg
      901 cgttctgcca acaacactgc attttcacgc gttacttgcg gaataaaaat agcaaactct
      961 tcgccgccga tacgcccaaa aatttcattc tcgcgcagcg cggcacggat gcgcgtcgct
     1021 gtttctttt
//
LOCUS       DAOMPD010000731         1026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_50424_length_1026_cov_3.184346, whole genome shotgun sequence.
ACCESSION   DAOMPD010000731 DAOMPD010000000
VERSION     DAOMPD010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50424_length_1026_cov_3.184346"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..671
                     /locus_tag="PLY93_15850"
     CDS             153..671
                     /locus_tag="PLY93_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70996.1"
                     /translation="MQCTLSKSLLSLIFLTTLYAETKATNAPQNEMDAYRAILTRPTA
                     TRQDLYDLVAMQDGVFEKYPQAAERNRYLSQKGITIKEETNTEVSRGEVAKVTLQHFN
                     LEKGILFWLTGWEFYALRDIQQLDIMQQKFTVHQALSGKQLIGIWSTAVATAERRDNY
                     SQNSGAQNEETQ"
     gene            701..>1026
                     /locus_tag="PLY93_15855"
     CDS             701..>1026
                     /locus_tag="PLY93_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP70997.1"
                     /translation="MKHTIKILLITLILVGASAWAVPVGAFMHIKGNVQVKPENGKWT
                     KATVGMKFDAKTEIQTALTGSAVVQFVNGTVMNIASGTSVGIARMVSGSYGTATDVQL
                     SLGRIKT"
BASE COUNT          323 a          217 c          242 g          244 t
ORIGIN      
        1 ccatcgatgc gctctataca ctagaagcct cacagattgc ccaccttttg ccgcgcgtgc
       61 ctgcttcaaa attgaaagag aatattcaag gtacgacgag tatcttttaa aaaatacttt
      121 acagcatgaa tagtgcatcc ctgccattgg atatgcagtg tacactgtct aaaagcctat
      181 tgagtttaat ttttctgaca acactctatg cagagacaaa agcaacaaat gcgccacaaa
      241 acgagatgga cgcatatcgt gccattttaa cgcgccccac agcaacgcgc caagatttat
      301 acgatttagt cgccatgcaa gacggcgtgt ttgaaaaata cccacaagcg gctgagcgga
      361 accggtatct atcccaaaaa ggaataacga ttaaagaaga aacaaatacc gaagtctcac
      421 ggggagaagt ggcgaaagtt acgctacagc attttaattt agagaaggga atactcttct
      481 ggttaaccgg ttgggaattt tatgcgttga gagatattca acaattagac atcatgcagc
      541 agaagtttac ggtacaccag gcactttcgg gaaagcagct tatcggcatt tggagcacgg
      601 cggtcgccac cgctgaacgt agagataatt attcgcaaaa tagcggtgca caaaacgaag
      661 agacgcaata aattattgat tacttgctaa agggggattt atgaaacata caattaaaat
      721 tttgctgatt acattgattt tggtgggtgc atctgcgtgg gcggtgccag tgggtgcatt
      781 catgcatatt aaaggcaatg tgcaagtaaa acccgaaaac ggtaaatgga caaaagccac
      841 ggtgggtatg aaattcgacg ctaaaaccga aattcaaacc gcactaacag ggagtgctgt
      901 cgtccaattt gtaaacggta ccgtcatgaa tatcgcctcg ggcaccagcg ttggcattgc
      961 gcgcatggtg tcgggttcgt atggcaccgc gaccgatgta caactgagtt tagggcgtat
     1021 caaaac
//
LOCUS       DAOMPD010000732         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_50627_length_1023_cov_2.247934, whole genome shotgun sequence.
ACCESSION   DAOMPD010000732 DAOMPD010000000
VERSION     DAOMPD010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50627_length_1023_cov_2.247934"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLY93_15860"
     CDS             complement(<1..1004)
                     /locus_tag="PLY93_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRP70998.1"
                     /translation="MDAKGQFKAPAGAYNLAGDGVMDLDAIGERLHKPVRYWSPLFLK
                     ILFAILKPLRLSRYGSEQVRFLEYRPVLDNTRLKKVFGFKPQKTSREAFEFYRIKHMR
                     SSLAESVFVLTGVAGGLGESLALALGARGATIIGVDSNKEALPRVSTALSSQKIKHQL
                     SALDITDKRAVEKFCKPFQEKRIDGLILNAGITRIFFFDELSDAEFEKVFAVNFFAAV
                     SLCRHLLKPLRRSSGVLVGISSVSGFAPLMKRTAYSASKHALAGFLETLRSEETAIDV
                     LVVYPSFIETQLRQNSEAGTAAAGKPKGISAEAAAQKICTAVVDRKKRLYLPFLAKLS
                     R"
BASE COUNT          271 a          268 c          233 g          251 t
ORIGIN      
        1 cgtgaaagct ttgcgaggaa aggtaagtac aaccttttct tgcggtcgac aactgcagta
       61 caaattttct gagccgccgc ctctgccgaa atgcctttcg gttttcccgc cgctgctgtg
      121 cccgcttctg aattttgccg taattgcgtt tcgataaacg acggataaac caccaacacg
      181 tcgattgccg tttcttcgct gcgcagcgtt tctaaaaaac ccgccaaagc gtgcttcgag
      241 gccgagtacg cggtacgttt catgagcggc gcaaaaccgc tcacgctcga tatacccact
      301 aacacaccgc tggagcggcg cagcggttta aggagatgac ggcagagcga caccgcagca
      361 aagaaattca ccgcaaaaac tttttcaaat tcggcatccg ataattcgtc gaagaaaaaa
      421 atccgagtaa tacctgcatt gaggattagc ccgtcgatgc gtttttcttg gaacggttta
      481 caaaacttct cgaccgctct cttgtcggtg atgtcgagtg cagagagttg atgtttaatt
      541 ttttgtgacg agagtgcggt actaacgcgc ggcaacgctt ctttattact gtcaacgccg
      601 atgattgtcg ccccccgtgc acccaatgcc aacgccaacg attctcccaa accaccggca
      661 acaccggtaa gtacaaaaac agattctgca agagatgacc gcatatgttt tatacgatag
      721 aactcgaacg cttcacgact tgttttttgg ggcttaaagc caaatacttt tttaagacgc
      781 gtattatcca aaacaggccg gtactctaaa aagcgcactt gttcgctgcc ataacgtgaa
      841 agacgcagcg gtttcaaaat tgcaaataaa attttaagaa acaagggtga ccaatagcgg
      901 acaggtttat gtagacgctc gccgattgcg tctaaatcca tgacaccgtc gccggctaaa
      961 ttatacgccc ccgccggcgc tttgaattga cctttcgcat ccatagccgc cgcgtgtgtt
     1021 aga
//
LOCUS       DAOMPD010000733         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_50815_length_1020_cov_1.848705, whole genome shotgun sequence.
ACCESSION   DAOMPD010000733 DAOMPD010000000
VERSION     DAOMPD010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50815_length_1020_cov_1.848705"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="PLY93_15865"
     CDS             <1..>1020
                     /locus_tag="PLY93_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRP70999.1"
                     /translation="QWIDTPYEIPSLRSYAKSYLDGRGWDGSLLDDLSNFNLQPFESV
                     HIPESCLTAPCNSIALPTDSIQYGYPGLTLSNKSASGSAWVDAQVEHCAANPTISFPQ
                     SRLNAVQATDLRASTDFALQKNMSVGCDRRDCRRVCFIFCGTICLSYPGICTLEFSAT
                     GHVDVGKNMPLAKAAANDWRTVAENLANVNYSFTWYAKLVGGVNQSQGNSYFNQTVEA
                     LRNQLVTTGREIGRGMGESFRSAVNNAGIHDPVFNEDNCFVTTNPQDTVVFQPANGTG
                     GIPLISTISLTFSEAILPHSLQVSTTHDCTGNVLLSSDNFATCHPFSTTAAQMSNENK
                     TATVTP"
BASE COUNT          256 a          260 c          252 g          252 t
ORIGIN      
        1 gcagtggatt gacacgccgt atgaaatacc gtcgttgcgt agctatgcaa aaagttattt
       61 agatgggcgc ggttgggacg gctctttact cgacgatctt tctaacttta atttacaacc
      121 ttttgaaagc gttcatatac ccgagtcgtg cctcactgcg ccctgcaaca gcattgcgtt
      181 gcccaccgat tcgattcaat acggttatcc gggtttaacg ttaagcaata aatcggcttc
      241 gggcagtgcg tgggtcgacg cgcaagtaga gcattgcgcg gcaaacccga cgatttcgtt
      301 tccacaaagc cgcttgaatg cagtgcaggc aaccgacttg cgtgccagta ccgattttgc
      361 attacagaaa aatatgtccg ttggctgcga taggcgcgat tgtagaagag tatgttttat
      421 cttttgtgga actatctgtt taagctaccc gggaatatgt accttagaat tctcggcgac
      481 agggcacgtc gatgtcggta aaaatatgcc gcttgccaaa gccgccgcca acgattggcg
      541 caccgtggct gaaaacttag cgaatgtaaa ctattcgttt acttggtatg caaagctggt
      601 gggcggcgtc aaccaaagcc agggcaactc gtatttcaac caaaccgtcg aagccttgcg
      661 caaccaactg gtcacaaccg gtcgtgaaat cggccggggt atgggtgaaa gctttcgctc
      721 ggcggtaaat aacgcgggca ttcacgaccc cgtgttcaac gaagataatt gctttgttac
      781 cactaacccg caagataccg tcgtctttca acccgccaac ggtaccggcg gcataccgct
      841 tatatcgacg attagtctca cgttctccga agcgatttta ccgcattcgt tgcaagtttc
      901 tacgacacac gattgcacgg gtaatgtatt gttatcgagc gacaatttcg ccacctgcca
      961 cccgtttagc acgacggctg cgcagatgtc aaacgaaaat aaaacggcga cagttacgcc
//
LOCUS       DAOMPD010000734         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_51528_length_1009_cov_3.088050, whole genome shotgun sequence.
ACCESSION   DAOMPD010000734 DAOMPD010000000
VERSION     DAOMPD010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51528_length_1009_cov_3.088050"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLY93_15870"
     CDS             complement(<1..368)
                     /locus_tag="PLY93_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRP71000.1"
                     /translation="MQAIDPFFNVPRESVKTSRGDVELPILYYELEILIALFIIDSKP
                     AKDMLASVGLKPVELPLGKTVGGLAFYRYDNTTVGIYNEVGVGIAAVPVEQKIPLLSI
                     TDFLKPTLKRRLGFYIADLPV"
     gene            complement(385..>1009)
                     /locus_tag="PLY93_15875"
     CDS             complement(385..>1009)
                     /locus_tag="PLY93_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRP71001.1"
                     /translation="EKEDIDSWTCFLVQKEVKGFSAGRNELKMGQRASSATELIFEDV
                     FVPSSHVIGGLRNGWSLSRTVLNYSRIPVAAIALGIARGAFEASIDFATTHALASRKL
                     IEYQEVQLSLARMFTLTNAMRSMVWSNAAHWTARQAYASATKVFCSDTAMQVADLAME
                     LMGNHGLEQRTYAEKALRDARLTQIYEGTNQINLLGIIEDQYTEFFK"
BASE COUNT          252 a          258 c          240 g          259 t
ORIGIN      
        1 actggtagat ctgcgatgta aaaacccaag cgccgcttaa gggttggctt taaaaaatct
       61 gttatcgaca acagtgggat tttctgttct accggcaccg cagcaatacc tacccccact
      121 tcattatata tacccacggt ggtgttatca tagcgataaa acgccagacc acccaccgtc
      181 ttgccgagag gcaactccac cggttttaat cccactgaag cgagcatatc ttttgccggt
      241 ttactatcga tgataaaaag agcgatgagg atttctaact cgtaatagag gatggggagt
      301 tcgacatccc ctcgactggt tttgacactt tcgcgcggaa cgttgaaaaa agggtcgatt
      361 gcttgcatag ttatctcctt aattttactt aaaaaattct gtgtattggt cttcgataat
      421 accgagtaaa ttgatttggt tggtgccttc ataaatttga gtgaggcgtg catcgcgcag
      481 cgctttctct gcgtaggtac gttgctccag accatgattc cccatgagct ccatcgcaag
      541 gtctgccact tgcattgcgg tatcggagca aaaaactttg gtcgccgatg cgtacgcttg
      601 ccgtgccgtc caatgcgcag cgttactcca caccatcgag cgcatcgcat tggtgagggt
      661 gaacatccgc gcgagagaaa gctgcacttc ttggtattca atgagtttgc gcgatgcgag
      721 ggcatgcgtc gttgcaaagt cgatggacgc ctcaaatgca ccgcgggcaa tccccaacgc
      781 aatcgccgcg accggtatgc gcgaataatt gagcaccgta cggctcaacg accaaccgtt
      841 ccgcaaacca ccaatgacat gcgaagaggg gacgaacaca tcttcaaaaa taagttctgt
      901 ggctgaggac gcccgctgtc ccattttaag ttcgttgcgt ccagcgctaa aacctttcac
      961 ttctttttgg acgagaaagc acgtccatga atcgatgtct tctttttcg
//
LOCUS       DAOMPD010000735         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_51659_length_1007_cov_2.877101, whole genome shotgun sequence.
ACCESSION   DAOMPD010000735 DAOMPD010000000
VERSION     DAOMPD010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51659_length_1007_cov_2.877101"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLY93_15880"
     CDS             complement(<1..218)
                     /locus_tag="PLY93_15880"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRP71002.1"
                     /translation="MPQQYSVAEVCQIIDKKIKASPELQEVWVRGEITNYQRHASSGH
                     IYITLSDVSQKTTNQRSTLRCTYFKFAQT"
     gene            320..>1007
                     /locus_tag="PLY93_15885"
     CDS             320..>1007
                     /locus_tag="PLY93_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyltransferase"
                     /protein_id="HRP71003.1"
                     /translation="MQPILNAPLVVRLKGTQKEMGGQYGEILAAQGGYQDLLEFYPHM
                     AENLLVSGLPRRARTKTARFIASQLIQTAARSLSKYRNDEYAARQSALLKAAGITEAL
                     AQQQLVMDVFQNSIGVLGRFGILHANERAYLNAIPACTSAVVWDSLSRDGELMHARNF
                     DFPGVGVWDKIPTIVYSTPDKGIPYGYIGARGADVPGITAFNSEGLSVAFHTRFHRDV
                     DFNALGVIDLG"
BASE COUNT          246 a          253 c          250 g          258 t
ORIGIN      
        1 gtttgtgcaa acttaaaata cgtacaacgc aacgtagagc gttggttggt tgttttctgg
       61 cttacatccg aaagtgtgat gtagatatgc ccagaagaag cgtgtctttg gtaattggtg
      121 atttcaccgc gcacccacac ttcttgcagt tcgggtgagg ctttaatttt tttatcgata
      181 atttgacaaa cttcggcgac gctgtactgc tgtggcattt ttaccttaat gaaaaatcgt
      241 tatgcgaagt aaatacaatg gagcctgcgc tatcggtttt ttattgacac ttgcatcgtt
      301 cgataagtaa tttaattata tgcaaccgat tttaaacgcc ccactcgttg tgcggctcaa
      361 gggcacgcaa aaggaaatgg gcggtcaata cggcgaaatc ctcgccgcgc agggaggcta
      421 ccaagacctg ctcgagtttt atcctcacat ggctgaaaat cttctcgtta gcggtttacc
      481 acgccgcgcc cgcaccaaga cggcgcgctt tatcgcatcg cagctcatac aaacagcggc
      541 gcgctctctc tcaaaatacc ggaacgacga atacgctgca aggcaaagtg cgctcttaaa
      601 agcggcggga attacagagg cactggcaca gcaacaactc gttatggatg tttttcaaaa
      661 cagtattgga gtcttaggcc gcttcggcat actccacgcc aacgaaagag catacctcaa
      721 cgcaatcccc gcatgtacct cggcggtcgt atgggattca ctttcgcgcg atggagaatt
      781 gatgcatgcg cgcaatttcg acttccccgg tgtgggggtg tgggataaaa tccccacaat
      841 cgtctattcg acaccggaca agggaattcc ttatggctac attggcgcac gaggggcgga
      901 cgtgccaggt atcacggcgt ttaactctga gggtctgagc gtcgcttttc acacccgttt
      961 ccaccgcgac gttgatttca acgctctcgg cgtcatcgat ttaggcc
//
LOCUS       DAOMPD010000736         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Turneriella sp. isolate AMR_MDS_5869
            NODE_51684_length_1007_cov_1.922269, whole genome shotgun sequence.
ACCESSION   DAOMPD010000736 DAOMPD010000000
VERSION     DAOMPD010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868090
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Turneriella sp. (wastewater metagenome)
  ORGANISM  Turneriella sp.
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae; Turneriella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.270
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:02:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,179
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,103
            CDSs (with protein)               :: 3,103
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 24 of 51
            Pseudo Genes (internal stop)      :: 9 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Turneriella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51684_length_1007_cov_1.922269"
                     /isolate="AMR_MDS_5869"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2717305"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLY93_15890"
     CDS             <1..608
                     /locus_tag="PLY93_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HRP71004.1"
                     /translation="AVGSLKEEIVPGHFVLPSQIIDRTRHRRDTYFDEGIVVHVAFGD
                     PMDAKLAGILKRALERLQLTVHANETLVCMEGPQFSTRAESKLYKSWGAGIINMSALP
                     EAKLARELELPYQMICMATDYDAWRDHDHAVTVEDVMRVVKTNSANAQKVLKEALGEL
                     AKEKGKLNSLVGSVKFGIMTQAEKRDKALCEKYNAVLPGYF"
     gene            611..>1007
                     /locus_tag="PLY93_15895"
     CDS             611..>1007
                     /locus_tag="PLY93_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRP71005.1"
                     /translation="MNKKEKPHLIIIGGGFGGLTLARSLKHAPIQITLIDKANHHLFQ
                     PLLYQVATAALSPGHIAMPLRAIFRSQKNVRVLMNRVAEILPKEKKIRLASGDEFTYD
                     RLVIAAGARHSYFGHDEWEKNAPGLKSVAD"
BASE COUNT          266 a          244 c          259 g          238 t
ORIGIN      
        1 ctgcggtggg ctctcttaaa gaagaaatcg tccctgggca ttttgtcttg ccgtctcaaa
       61 ttatcgatcg cacccgccac cgccgcgaca cgtattttga cgaggggatt gtcgtgcacg
      121 ttgcttttgg cgacccgatg gacgcaaaac ttgcgggtat tttgaagcgc gcattggagc
      181 gtcttcagtt gaccgtgcac gcgaatgaaa cgttggtgtg tatggaaggt ccacagtttt
      241 cgacgcgcgc cgagtcgaaa ctctataaat cttggggggc gggcatcatc aacatgagtg
      301 cgctccccga agcaaaactg gcgcgcgagt tagaattgcc ataccaaatg atttgcatgg
      361 caaccgacta cgacgcgtgg cgcgaccacg accatgcggt taccgtcgaa gacgtgatgc
      421 gcgtggtgaa aaccaattcc gccaatgcgc aaaaagtact taaagaagcg ctcggcgaat
      481 tggccaaaga aaaaggtaag ctcaactcgc ttgtgggctc ggttaaattt ggtatcatga
      541 cgcaagcaga aaagcgcgac aaagcattgt gtgaaaaata taacgctgtt ttgccgggat
      601 atttttgatt atgaataaaa aagaaaaacc acatttaatt atcatcggtg gtggtttcgg
      661 cggattgacc ctagcgcgtt cgttgaaaca tgcaccaatt caaatcactc tcatcgataa
      721 agccaatcat catctttttc aaccgctcct ctaccaagtt gccacggctg cactttcgcc
      781 tgggcacatc gccatgcctt tacgcgccat ttttcgttcg caaaaaaacg tgcgggtatt
      841 gatgaaccgt gtcgctgaaa ttttacccaa agagaaaaaa atccgcctgg cgtcgggcga
      901 tgaattcacg tacgatcgac tcgtcattgc agcgggagcg cggcattcgt attttgggca
      961 cgatgaatgg gaaaaaaatg ccccgggact taaaagcgtc gccgatg
//
